LOCUS       DAYPHN010000001        92187 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000001 DAYPHN010000000
VERSION     DAYPHN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 92187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.001"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            271..1839
                     /gene="cydA"
                     /locus_tag="ACG0BW_000001"
     CDS             271..1839
                     /gene="cydA"
                     /locus_tag="ACG0BW_000001"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFK7257030.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1855..2994
                     /gene="cydB"
                     /locus_tag="ACG0BW_000002"
     CDS             1855..2994
                     /gene="cydB"
                     /locus_tag="ACG0BW_000002"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFK7257031.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3009..3122
                     /gene="cydX"
                     /locus_tag="ACG0BW_000003"
     CDS             3009..3122
                     /gene="cydX"
                     /locus_tag="ACG0BW_000003"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFK7257032.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3122..3415
                     /gene="ybgE"
                     /locus_tag="ACG0BW_000004"
     CDS             3122..3415
                     /gene="ybgE"
                     /locus_tag="ACG0BW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFK7257033.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3565..3969
                     /gene="ybgC"
                     /locus_tag="ACG0BW_000005"
     CDS             3565..3969
                     /gene="ybgC"
                     /locus_tag="ACG0BW_000005"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFK7257034.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3966..4658
                     /gene="tolQ"
                     /locus_tag="ACG0BW_000006"
     CDS             3966..4658
                     /gene="tolQ"
                     /locus_tag="ACG0BW_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFK7257035.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4662..5090
                     /gene="tolR"
                     /locus_tag="ACG0BW_000007"
     CDS             4662..5090
                     /gene="tolR"
                     /locus_tag="ACG0BW_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFK7257036.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5155..6420
                     /gene="tolA"
                     /locus_tag="ACG0BW_000008"
     CDS             5155..6420
                     /gene="tolA"
                     /locus_tag="ACG0BW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFK7257037.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAVEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6553..7845
                     /gene="tolB"
                     /locus_tag="ACG0BW_000009"
     CDS             6553..7845
                     /gene="tolB"
                     /locus_tag="ACG0BW_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFK7257038.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7880..8401
                     /gene="pal"
                     /locus_tag="ACG0BW_000010"
     CDS             7880..8401
                     /gene="pal"
                     /locus_tag="ACG0BW_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFK7257039.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8411..9202
                     /gene="cpoB"
                     /locus_tag="ACG0BW_000011"
     CDS             8411..9202
                     /gene="cpoB"
                     /locus_tag="ACG0BW_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFK7257040.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAYAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9367..9442
                     /locus_tag="ACG0BW_000012"
     tRNA            9367..9442
                     /locus_tag="ACG0BW_000012"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9400..9402,aa:Lys,seq:ttt)
     gene            9578..9653
                     /locus_tag="ACG0BW_000013"
     tRNA            9578..9653
                     /locus_tag="ACG0BW_000013"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9611..9613,aa:Val,seq:tac)
     gene            9656..9731
                     /locus_tag="ACG0BW_000014"
     tRNA            9656..9731
                     /locus_tag="ACG0BW_000014"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9689..9691,aa:Lys,seq:ttt)
     gene            9783..9858
                     /locus_tag="ACG0BW_000015"
     tRNA            9783..9858
                     /locus_tag="ACG0BW_000015"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9816..9818,aa:Val,seq:tac)
     gene            9862..9937
                     /locus_tag="ACG0BW_000016"
     tRNA            9862..9937
                     /locus_tag="ACG0BW_000016"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9895..9897,aa:Lys,seq:ttt)
     gene            10084..10159
                     /locus_tag="ACG0BW_000017"
     tRNA            10084..10159
                     /locus_tag="ACG0BW_000017"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10117..10119,aa:Lys,seq:ttt)
     gene            10193..10268
                     /locus_tag="ACG0BW_000018"
     tRNA            10193..10268
                     /locus_tag="ACG0BW_000018"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10226..10228,aa:Lys,seq:ttt)
     gene            10299..10424
                     /locus_tag="ACG0BW_000019"
     ncRNA           10299..10424
                     /ncRNA_class="other"
                     /locus_tag="ACG0BW_000019"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10455..10580
                     /locus_tag="ACG0BW_000020"
     ncRNA           10455..10580
                     /ncRNA_class="other"
                     /locus_tag="ACG0BW_000020"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10858..11901
                     /gene="nadA"
                     /locus_tag="ACG0BW_000021"
     CDS             10858..11901
                     /gene="nadA"
                     /locus_tag="ACG0BW_000021"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFK7257041.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSSEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSTAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVEERLRERALVP
                     LNRMLDFAATLRG"
     gene            11939..12658
                     /gene="pnuC"
                     /locus_tag="ACG0BW_000022"
     CDS             11939..12658
                     /gene="pnuC"
                     /locus_tag="ACG0BW_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFK7257042.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQILLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12655..13596)
                     /gene="zitB"
                     /locus_tag="ACG0BW_000023"
     CDS             complement(12655..13596)
                     /gene="zitB"
                     /locus_tag="ACG0BW_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFK7257043.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13710..14090)
                     /gene="ybgS"
                     /locus_tag="ACG0BW_000024"
     CDS             complement(13710..14090)
                     /gene="ybgS"
                     /locus_tag="ACG0BW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFK7257044.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14406..15458
                     /gene="aroG"
                     /locus_tag="ACG0BW_000025"
     CDS             14406..15458
                     /gene="aroG"
                     /locus_tag="ACG0BW_000025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFK7257045.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRHLANAVKARRG"
     gene            complement(15624..16376)
                     /gene="gpmA"
                     /locus_tag="ACG0BW_000026"
     CDS             complement(15624..16376)
                     /gene="gpmA"
                     /locus_tag="ACG0BW_000026"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFK7257046.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16579..17619)
                     /gene="galM"
                     /locus_tag="ACG0BW_000027"
     CDS             complement(16579..17619)
                     /gene="galM"
                     /locus_tag="ACG0BW_000027"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFK7257047.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPLDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17613..18761)
                     /gene="galK"
                     /locus_tag="ACG0BW_000028"
     CDS             complement(17613..18761)
                     /gene="galK"
                     /locus_tag="ACG0BW_000028"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFK7257048.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDHKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18765..19811)
                     /gene="galT"
                     /locus_tag="ACG0BW_000029"
     CDS             complement(18765..19811)
                     /gene="galT"
                     /locus_tag="ACG0BW_000029"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFK7257049.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19821..20837)
                     /gene="galE"
                     /locus_tag="ACG0BW_000030"
     CDS             complement(19821..20837)
                     /gene="galE"
                     /locus_tag="ACG0BW_000030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFK7257050.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21100..22572)
                     /gene="modF"
                     /locus_tag="ACG0BW_000031"
     CDS             complement(21100..22572)
                     /gene="modF"
                     /locus_tag="ACG0BW_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFK7257051.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETSA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            complement(22640..23428)
                     /gene="modE"
                     /locus_tag="ACG0BW_000032"
     CDS             complement(22640..23428)
                     /gene="modE"
                     /locus_tag="ACG0BW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFK7257052.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23557..23706
                     /gene="acrZ"
                     /locus_tag="ACG0BW_000033"
     CDS             23557..23706
                     /gene="acrZ"
                     /locus_tag="ACG0BW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFK7257053.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23873..24646
                     /gene="modA"
                     /locus_tag="ACG0BW_000034"
     CDS             23873..24646
                     /gene="modA"
                     /locus_tag="ACG0BW_000034"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7257054.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24646..25335
                     /gene="modB"
                     /locus_tag="ACG0BW_000035"
     CDS             24646..25335
                     /gene="modB"
                     /locus_tag="ACG0BW_000035"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFK7257055.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25338..26396
                     /gene="modC"
                     /locus_tag="ACG0BW_000036"
     CDS             25338..26396
                     /gene="modC"
                     /locus_tag="ACG0BW_000036"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFK7257056.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGVLEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26397..27215)
                     /gene="ybhA"
                     /locus_tag="ACG0BW_000037"
     CDS             complement(26397..27215)
                     /gene="ybhA"
                     /locus_tag="ACG0BW_000037"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFK7257057.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARACEAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27370..28365
                     /gene="pgl"
                     /locus_tag="ACG0BW_000038"
     CDS             27370..28365
                     /gene="pgl"
                     /locus_tag="ACG0BW_000038"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFK7257058.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28406..29359)
                     /gene="ybhD"
                     /locus_tag="ACG0BW_000039"
     CDS             complement(28406..29359)
                     /gene="ybhD"
                     /locus_tag="ACG0BW_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7257059.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29543..30595
                     /gene="ybhH"
                     /locus_tag="ACG0BW_000040"
     CDS             29543..30595
                     /gene="ybhH"
                     /locus_tag="ACG0BW_000040"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFK7257060.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVFLP"
     gene            30671..32104
                     /gene="ybhI"
                     /locus_tag="ACG0BW_000041"
     CDS             30671..32104
                     /gene="ybhI"
                     /locus_tag="ACG0BW_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFK7257061.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32287..34548
                     /gene="ybhJ"
                     /locus_tag="ACG0BW_000042"
     CDS             32287..34548
                     /gene="ybhJ"
                     /locus_tag="ACG0BW_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFK7257062.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34689..35972)
                     /gene="ybhC"
                     /locus_tag="ACG0BW_000043"
     CDS             complement(34689..35972)
                     /gene="ybhC"
                     /locus_tag="ACG0BW_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFK7257063.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36124..36600)
                     /gene="ybhB"
                     /locus_tag="ACG0BW_000044"
     CDS             complement(36124..36600)
                     /gene="ybhB"
                     /locus_tag="ACG0BW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFK7257064.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNVSPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(36659..37948)
                     /gene="bioA"
                     /locus_tag="ACG0BW_000045"
     CDS             complement(36659..37948)
                     /gene="bioA"
                     /locus_tag="ACG0BW_000045"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFK7257065.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVVAIEVQLREQLAPARDAKMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            38035..39075
                     /gene="bioB"
                     /locus_tag="ACG0BW_000046"
     CDS             38035..39075
                     /gene="bioB"
                     /locus_tag="ACG0BW_000046"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFK7257066.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39072..40226
                     /gene="bioF"
                     /locus_tag="ACG0BW_000047"
     CDS             39072..40226
                     /gene="bioF"
                     /locus_tag="ACG0BW_000047"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFK7257067.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SCNDYLGLSHHPDIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMTAKEDRIVADRLSHASLLEAASLSPAQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVNPELLVVTFGKGFGVSGAAVLCSDAVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            40213..40968
                     /gene="bioC"
                     /locus_tag="ACG0BW_000048"
     CDS             40213..40968
                     /gene="bioC"
                     /locus_tag="ACG0BW_000048"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFK7257068.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQVVDERP
                     HANRFLPPDKIEQSLNGLHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            40961..41638
                     /gene="bioD"
                     /locus_tag="ACG0BW_000049"
     CDS             40961..41638
                     /gene="bioD"
                     /locus_tag="ACG0BW_000049"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFK7257069.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRGLEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            42217..44238
                     /gene="uvrB"
                     /locus_tag="ACG0BW_000050"
     CDS             42217..44238
                     /gene="uvrB"
                     /locus_tag="ACG0BW_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFK7257070.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(44385..45293)
                     /gene="yvcK"
                     /locus_tag="ACG0BW_000051"
     CDS             complement(44385..45293)
                     /gene="yvcK"
                     /locus_tag="ACG0BW_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFK7257071.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDASAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      45558..45702
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            45690..46679
                     /gene="moaA"
                     /locus_tag="ACG0BW_000052"
     CDS             45690..46679
                     /gene="moaA"
                     /locus_tag="ACG0BW_000052"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFK7257072.1"
                     /translation="MASQLTDAFARKYYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            46701..47213
                     /gene="moaB"
                     /locus_tag="ACG0BW_000053"
     CDS             46701..47213
                     /gene="moaB"
                     /locus_tag="ACG0BW_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFK7257073.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            47216..47701
                     /gene="moaC"
                     /locus_tag="ACG0BW_000054"
     CDS             47216..47701
                     /gene="moaC"
                     /locus_tag="ACG0BW_000054"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFK7257074.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            47694..47939
                     /gene="moaD"
                     /locus_tag="ACG0BW_000055"
     CDS             47694..47939
                     /gene="moaD"
                     /locus_tag="ACG0BW_000055"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFK7257075.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            47941..48393
                     /gene="moaE"
                     /locus_tag="ACG0BW_000056"
     CDS             47941..48393
                     /gene="moaE"
                     /locus_tag="ACG0BW_000056"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFK7257076.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRITVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            48530..49234
                     /gene="ybhL"
                     /locus_tag="ACG0BW_000057"
     CDS             48530..49234
                     /gene="ybhL"
                     /locus_tag="ACG0BW_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFK7257077.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            49439..49828
                     /locus_tag="ACG0BW_000058"
     CDS             49439..49828
                     /locus_tag="ACG0BW_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257078.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGMVATAGLALVIIMN
                     AALMSERPVWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(49864..50820)
                     /gene="ybhN"
                     /locus_tag="ACG0BW_000059"
     CDS             complement(49864..50820)
                     /gene="ybhN"
                     /locus_tag="ACG0BW_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFK7257079.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(50820..52061)
                     /gene="clsB"
                     /locus_tag="ACG0BW_000060"
     CDS             complement(50820..52061)
                     /gene="clsB"
                     /locus_tag="ACG0BW_000060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFK7257080.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(52058..52819)
                     /gene="ybhP"
                     /locus_tag="ACG0BW_000061"
     CDS             complement(52058..52819)
                     /gene="ybhP"
                     /locus_tag="ACG0BW_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFK7257081.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            52952..53362
                     /gene="ybhQ"
                     /locus_tag="ACG0BW_000062"
     CDS             52952..53362
                     /gene="ybhQ"
                     /locus_tag="ACG0BW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFK7257082.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(53324..54430)
                     /gene="ybhR"
                     /locus_tag="ACG0BW_000063"
     CDS             complement(53324..54430)
                     /gene="ybhR"
                     /locus_tag="ACG0BW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7257083.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(54441..55574)
                     /gene="ybhS"
                     /locus_tag="ACG0BW_000064"
     CDS             complement(54441..55574)
                     /gene="ybhS"
                     /locus_tag="ACG0BW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7257084.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(55567..57303)
                     /gene="ybhF"
                     /locus_tag="ACG0BW_000065"
     CDS             complement(55567..57303)
                     /gene="ybhF"
                     /locus_tag="ACG0BW_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFK7257085.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(57296..58291)
                     /gene="hlyD"
                     /locus_tag="ACG0BW_000066"
     CDS             complement(57296..58291)
                     /gene="hlyD"
                     /locus_tag="ACG0BW_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFK7257086.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(58294..58965)
                     /gene="cecR"
                     /locus_tag="ACG0BW_000067"
     CDS             complement(58294..58965)
                     /gene="cecR"
                     /locus_tag="ACG0BW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFK7257087.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            59194..60558
                     /gene="rhlE"
                     /locus_tag="ACG0BW_000068"
     CDS             59194..60558
                     /gene="rhlE"
                     /locus_tag="ACG0BW_000068"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFK7257088.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVMEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPGEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(60794..61276)
                     /gene="ybiA"
                     /locus_tag="ACG0BW_000069"
     CDS             complement(60794..61276)
                     /gene="ybiA"
                     /locus_tag="ACG0BW_000069"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFK7257089.1"
                     /translation="MPVRAQRIQHVMQDTTINFYSTGDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATASAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            61396..63546
                     /gene="dinG"
                     /locus_tag="ACG0BW_000070"
     CDS             61396..63546
                     /gene="dinG"
                     /locus_tag="ACG0BW_000070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFK7257090.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGVLPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIITSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            63574..64536
                     /gene="ybiB"
                     /locus_tag="ACG0BW_000071"
     CDS             63574..64536
                     /gene="ybiB"
                     /locus_tag="ACG0BW_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFK7257091.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDHEGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            64677..65762
                     /gene="hcxB"
                     /locus_tag="ACG0BW_000072"
     CDS             64677..65762
                     /gene="hcxB"
                     /locus_tag="ACG0BW_000072"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFK7257092.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLRINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGIPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRLERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            65829..65888
                     /gene="ybiE"
                     /locus_tag="ACG0BW_000073"
     CDS             65829..65888
                     /gene="ybiE"
                     /locus_tag="ACG0BW_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFK7257093.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(65991..66251)
                     /gene="ybiJ"
                     /locus_tag="ACG0BW_000074"
     CDS             complement(65991..66251)
                     /gene="ybiJ"
                     /locus_tag="ACG0BW_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFK7257094.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNTNKLSGTAVIYK"
     gene            complement(66516..66782)
                     /gene="ybiI"
                     /locus_tag="ACG0BW_000075"
     CDS             complement(66516..66782)
                     /gene="ybiI"
                     /locus_tag="ACG0BW_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFK7257095.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPDYTGYNRRGSKDSQLR"
     gene            complement(66856..67533)
                     /gene="ybiX"
                     /locus_tag="ACG0BW_000076"
     CDS             complement(66856..67533)
                     /gene="ybiX"
                     /locus_tag="ACG0BW_000076"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFK7257096.1"
                     /translation="MMYHIPGVLSPKDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPHTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFDLDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(67575..69857)
                     /gene="fiu"
                     /locus_tag="ACG0BW_000077"
     CDS             complement(67575..69857)
                     /gene="fiu"
                     /locus_tag="ACG0BW_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFK7257097.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKIKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGMAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(70122..70382)
                     /gene="mcbA"
                     /locus_tag="ACG0BW_000078"
     CDS             complement(70122..70382)
                     /gene="mcbA"
                     /locus_tag="ACG0BW_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFK7257098.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            70658..71584
                     /gene="rlmF"
                     /locus_tag="ACG0BW_000079"
     CDS             70658..71584
                     /gene="rlmF"
                     /locus_tag="ACG0BW_000079"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFK7257099.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEATGSIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDVLNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(71581..73806)
                     /gene="ybiO"
                     /locus_tag="ACG0BW_000080"
     CDS             complement(71581..73806)
                     /gene="ybiO"
                     /locus_tag="ACG0BW_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFK7257100.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(74067..74789)
                     /gene="glnQ"
                     /locus_tag="ACG0BW_000081"
     CDS             complement(74067..74789)
                     /gene="glnQ"
                     /locus_tag="ACG0BW_000081"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFK7257101.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(74786..75445)
                     /gene="glnP"
                     /locus_tag="ACG0BW_000082"
     CDS             complement(74786..75445)
                     /gene="glnP"
                     /locus_tag="ACG0BW_000082"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFK7257102.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(75584..76330)
                     /gene="glnH"
                     /locus_tag="ACG0BW_000083"
     CDS             complement(75584..76330)
                     /gene="glnH"
                     /locus_tag="ACG0BW_000083"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFK7257103.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(76734..77237)
                     /gene="dps"
                     /locus_tag="ACG0BW_000084"
                     /gene_synonym="pexB"
     CDS             complement(76734..77237)
                     /gene="dps"
                     /locus_tag="ACG0BW_000084"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFK7257104.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(77536..78423)
                     /gene="rhtA"
                     /locus_tag="ACG0BW_000085"
     CDS             complement(77536..78423)
                     /gene="rhtA"
                     /locus_tag="ACG0BW_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFK7257105.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLLLIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            78658..78723
                     /locus_tag="ACG0BW_000086"
     CDS             78658..78723
                     /locus_tag="ACG0BW_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257106.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            78776..79291
                     /gene="ompX"
                     /locus_tag="ACG0BW_000087"
     CDS             78776..79291
                     /gene="ompX"
                     /locus_tag="ACG0BW_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFK7257107.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(79340..80923)
                     /gene="opgE"
                     /locus_tag="ACG0BW_000088"
     CDS             complement(79340..80923)
                     /gene="opgE"
                     /locus_tag="ACG0BW_000088"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFK7257108.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPGVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPMQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWNNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(81195..81323)
                     /gene="mntS"
                     /locus_tag="ACG0BW_000089"
     CDS             complement(81195..81323)
                     /gene="mntS"
                     /locus_tag="ACG0BW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFK7257109.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            81509..81976
                     /gene="mntR"
                     /locus_tag="ACG0BW_000090"
     CDS             81509..81976
                     /gene="mntR"
                     /locus_tag="ACG0BW_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFK7257110.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            81973..83091
                     /gene="ybiR"
                     /locus_tag="ACG0BW_000091"
     CDS             81973..83091
                     /gene="ybiR"
                     /locus_tag="ACG0BW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFK7257111.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGVGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLFVMLPAW"
     gene            complement(83149..84069)
                     /gene="ldtB"
                     /locus_tag="ACG0BW_000092"
     CDS             complement(83149..84069)
                     /gene="ldtB"
                     /locus_tag="ACG0BW_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFK7257112.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            84288..85883
                     /gene="ybiT"
                     /locus_tag="ACG0BW_000093"
     CDS             84288..85883
                     /gene="ybiT"
                     /locus_tag="ACG0BW_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFK7257113.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIDG"
     gene            complement(86063..88426)
                     /locus_tag="ACG0BW_000094"
     CDS             complement(86063..88426)
                     /locus_tag="ACG0BW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFK7257114.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYEKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPVEEKRTLLVNGVEGSEWMR"
     gene            complement(88441..89742)
                     /locus_tag="ACG0BW_000095"
     CDS             complement(88441..89742)
                     /locus_tag="ACG0BW_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7257115.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSNLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHNPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            89980..91140
                     /locus_tag="ACG0BW_000096"
     CDS             89980..91140
                     /locus_tag="ACG0BW_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFK7257116.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAQQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            complement(91107..>92187)
                     /locus_tag="ACG0BW_000097"
     CDS             complement(91107..>92187)
                     /locus_tag="ACG0BW_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFK7257117.1"
                     /translation="VLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGWDQSM
                     LDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANVQSFLNR
                     LWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQHVFA
                     NVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLLHVVP
                     IPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAGDSVW
                     WHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFPREDY
                     ETNWEGRFTLADLNIHGKRALGIDS"
BASE COUNT        22037 a        23273 c        24677 g        22200 t
ORIGIN      
        1 acaatttcgc tataaattga tcactgtcga aaaatgcaaa tttgctcaat aaaaacctgt
       61 ttattgtaag gattttgcgg cgtaatataa ttacgggatc aatttggttt attcattaac
      121 atatttgtaa cttttttatc attgtctttt cctcttttga tgaaatggca acgatagagg
      181 cgagcaaata attttgtatt ggggcatgcg tgtgaccctt tctaacgggg ttcactctcg
      241 gagtcttcat gcgatgagca aggagtcatg atgttagata tagtcgaact gtcgcgctta
      301 cagtttgcct tgaccgcgat gtaccacttc ctttttgtgc cactgacgct cggtatggcg
      361 ttcctgctgg ccattatgga aacggtctac gtcctttccg gcaaacagat ttataaagat
      421 atgaccaagt tctggggcaa gttgtttggt atcaacttcg ctctgggtgt ggctaccggt
      481 ctgaccatgg agttccagtt cgggactaac tggtcttact attcccacta tgtaggggat
      541 atcttcggtg cgccgctggc aatcgaaggt ctaatggcct tcttcctcga atccaccttt
      601 gtaggtctgt tcttcttcgg ttgggatcgt ctgggtaaag ttcagcatat gtgtgtcacc
      661 tggctggtgg cgctcggttc aaacctgtcc gcactgtgga ttctggttgc gaacggctgg
      721 atgcaaaacc caatcgcgtc cgatttcaac tttgaaacta tgcgtatgga gatggtgagc
      781 ttctccgagc tggtgcttaa cccggttgct caggtgaaat tcgttcacac tgtagcgtct
      841 ggttatgtga ctggcgcgat gttcatcctc ggtatcagcg catggtatat gctgaaaggt
      901 cgtgacttcg ccttcgctaa acgctccttt gctatcgctg ccagcttcgg tatggctgcc
      961 gttctgtctg ttattgttct gggtgatgaa tctggttacg aaatgggcga cgtgcagaaa
     1021 accaaactgg ctgctattga agccgagtgg gaaacgcaac ctgcgcctgc tgcctttact
     1081 ctgttcggca ttcctgatca ggaagaggag acgaacaaat ttgcgatcca gatcccttac
     1141 gcgctgggca tcattgcaac gcgttccgtg gatactccag ttatcggcct gaaagaactg
     1201 atggtgcagc atgaagaacg cattcgtaac gggatgaagg cgtactctct gctcgagcaa
     1261 ctgcgttctg gttctaccga ccaggcggtt cgtgaccagt tcaatagcat gaagaaagac
     1321 ctcggttacg gtctgctgct gaaacgctat acgccaaacg tggctgatgc gactgaagcg
     1381 cagattcaac aggcaaccaa agactccatt ccgcgtgtag cgccgctgta cttcgcgttc
     1441 cgtatcatgg tggcgtgtgg cttcctgctg ctggcaatca tcgcgctctc tttctggagt
     1501 gtcatccgca accgcattgg cgagaaaaaa tggctgctgc gcgccgcgct gtacggtatt
     1561 ccgctgccgt ggattgctgt agaagcgggc tggttcgtgg ctgaatatgg ccgccaaccg
     1621 tgggctatcg gtgaagtgct gccgacagct gtggcgaact cgtcactgac cgcaggcgat
     1681 ctcatcttct caatggtgct gatttgcggc ctgtataccc tgttcctggt ggcagaattg
     1741 ttcttaatgt tcaagtttgc acgcctcggc ccaagcagcc tgaaaaccgg tcgctatcac
     1801 tttgagcagt cttccacgac tactcagccg gcacgctaag acaggagtcg tcaaatgatc
     1861 gattatgaag tattgcgttt tatctggtgg ctgctggttg gcgttctgct gattggtttt
     1921 gcagtcactg acggtttcga catgggggtg ggcatgctca cccgtttcct cggtcgtaac
     1981 gacaccgagc gtcgaattat gattaactcc attgcaccac actgggacgg taaccaggtt
     2041 tggcttatca ccgcgggcgg cgctctgttt gctgcctggc cgatggtcta tgccgctgcg
     2101 ttctccggat tctatgtggc gatgatcctc gtgctggcgt ctttgttctt ccgtccggtc
     2161 ggttttgact accgctccaa gattgaagaa acccgctggc gtaacatgtg ggactggggc
     2221 atcttcattg gtagcttcgt tccgccgctg gtgattggtg tggcgttcgg taacctgttg
     2281 cagggcgtac cgttcaacgt tgatgaatat ctgcgtctgt actacaccgg taacttcttc
     2341 cagctgctta acccgttcgg cctgctggca ggcgtggtga gcgtagggat gatcattact
     2401 cagggcgcaa cctatctgca aatgcgtacc gtgggcgaac tgcacctgcg tacccgtgca
     2461 acggctcagg tggcggcact ggtgacactg gtctgtttcg cactggctgg cgtatgggtg
     2521 atgtacggta tcgatggtta tgtcgtgaaa tcgacaatgg accattacgc agcctctaac
     2581 ccactgaata aagaagtggt tcgtgaagct ggcgcatggc tggtaaactt caacaacacg
     2641 ccaattctgt gggctattcc ggcactgggt gtggttctgc cgctgctgac catcctgact
     2701 gcacgtatgg ataaagccgc gtgggcgttt gtgttctcct ccctgacgct ggcctgtatc
     2761 atcctgactg ccggtatcgc aatgttcccg tttgtgatgc cgtccagcac catgatgaac
     2821 gcaagtctga caatgtggga tgcaacatcc agccagctga cgcttaacgt catgacctgg
     2881 gttgcggtgg ttctggtacc gatcattctg ctctacaccg cctggtgtta ctggaaaatg
     2941 ttcggtcgta tcaccaaaga agatattgaa cgtaacaccc actctctgta ctaagtaagg
     3001 agctaaaaat gtggtatttc gcatggattc tgggaacgct tcttgcctgt tcgtttgggg
     3061 taatcaccgc gctggcgctt gagcacgtcg aatcaggcaa agccggtcaa gaagacatct
     3121 gatgagtaag attatcgcaa ccttgtatgc ggtaatggac aagcgccccc tgcgggcgct
     3181 ttccttcgtg atggcgcttc tgttagcagg atgtatgttt tgggacccat cacgtttcgc
     3241 cgcgaagacc agtgaactgg aaatctggca tggtttattg ctgatgtggg ccgtctgtgc
     3301 tggtgtgatt cacggcgtgg gctttcgtcc gcagaaggtt ctttggcaag ggattttttg
     3361 cccattgctt gccgatattg ttctcattgt cgggctgatt ttcttcttct tttaaatcag
     3421 aattctcttt aaaaaattat gggccgctcc aggcccataa atttttacgc tcccttaact
     3481 tgccctcatt cccaaacctc aatcgcgcgc gtatagtagc agcgtttaaa agctctaact
     3541 tttgttgcat taccgggatg taaagtgaat acaacgctgt ttcgatggcc ggttcgcgtc
     3601 tactatgaag ataccgatgc cggtggtgtg gtgtaccacg ccagttacgt cgctttttat
     3661 gaaagagcac gcacagagat gctgcgtcat catcacttca gtcaacaggc gctgatggct
     3721 gaacgcgttg cctttgtggt acgtaaaatg acggtggaat attacgcacc tgcgcggctc
     3781 gacgatatgc tcgaaataca gactgaaata acatcaatgc gtggcacctc tttggttttc
     3841 acgcaacgta ttgtcaacgc cgagaatact ttgctgaatg aagcagaggt tctggttgtt
     3901 tgcgttgacc cactcaaaat gaagcctcgt gcgcttccca agtctattgt cgcggagttt
     3961 aagcagtgac tgacatgaat atccttgatt tgttcctgaa ggctagcctt ctggttaaac
     4021 ttatcatgtt gattttgatt ggtttttcaa tcgcatcctg ggccattatt atccagcgga
     4081 cccgtattct taacgcagcg gcgcgtgaag ccgaagcgtt tgaagataaa ttctggtctg
     4141 gaatcgaact ctctcgcctc tatcaagaga gccaggggaa acgggataat ctgactggtt
     4201 cggaacaaat cttttacagc gggttcaaag agtttgtgcg cctgcatcgt gccaatagcc
     4261 atgcgccgga agccgtagtg gaaggggcgt cgcgtgctat gcgtatctcc atgaaccgtg
     4321 aacttgaaaa tctggaaaca cacattccgt tcctcggtac ggttggctcc atcagcccgt
     4381 atattggtct gtttggtacg gtctggggga tcatgcacgc ctttatcgcc ctcggggcgg
     4441 taaaacaagc aacactgcaa atggttgcgc ccggtatcgc agaagcgttg attgcgacgg
     4501 caattggtct gttcgccgca attccggcgg taatggctta taaccgtctc aaccagcgcg
     4561 taaacaaact ggaactgaat tacgacaact ttatggaaga gtttaccgcg attctgcacc
     4621 gccaggcgtt taccgttagc gagagcaaca aggggtaagc catggccaga gcgcgtggac
     4681 gaggtcgtcg cgatctcaag tccgaaatca acattgtacc gttgctggac gtactgctgg
     4741 tgctgttgct gatctttatg gcgacagcgc ccatcatcac ccagagcgtg gaggtcgatc
     4801 tgccagacgc tactgaatca caggcggtga gcagtaacga taatccgcca gtgattgttg
     4861 aagtgtctgg tattggtcag tacaccgtgg tggttgagaa agatcgcctg gagcgtttgc
     4921 caccagagca ggtggtggcg gaagtgtcca gccgtttcaa ggccaacccg aaaacggtct
     4981 ttctgatcgg tggcgcaaaa gatgtgcctt acgatgaaat aattaaagca ctgaacttgt
     5041 tacatagtgc gggtgtgaaa tcggttggtt taatgacgca gcctatctaa acatctgcgt
     5101 ttcccttgct tgaaagagag cgggtaacag gcgaacagtt tttggaaacc gagagtgtca
     5161 aaggcaaccg aacaaaacga caagctcaag cgggcgataa ttatttcagc agtgctgcat
     5221 gtcatcttat ttgcggcgct gatctggagt tcgttcgatg agaatataga agcttcagcc
     5281 ggaggcggcg gtggttcatc catcgacgct gtcatggttg attcaggtgc ggtagttgag
     5341 cagtacaaac gcatgcaaag ccaggaatca agcgcgaagc gttctgatga gcagcgcaag
     5401 atgaaggaac agcaggctgc tgaagaactg cgtgagaaac aagcggctga acaggaacgc
     5461 ctgaagcaac ttgagaaaga gcggttagct gctcaggaac agaaaaagca ggctgaagaa
     5521 gccgcaaaac aggccgagtt aaagcagaag caagcggaag aagcggcagc gaaagcggcg
     5581 gcagatgcta aagcgaaggc cgaagcggat gctaaagctg cggaagaagc tgcgaagaaa
     5641 gcggctgcag acgcgaagaa aaaagcagaa gcagaagccg ccaaagccgc agccgaagcg
     5701 cagaaaaaag ccgaggcagc cgcggcggca ctgaagaaga aagcggaagc ggtagaagca
     5761 gctgcagctg aagcaagaaa gaaagcggca actgaagctg ctgaaaaagc caaagcagaa
     5821 gctgagaaga aagcggctgc tgaaaaggct gcagctgata agaaagcggc agcagagaaa
     5881 gctgcagccg acaaaaaagc agcagaaaaa gcggctgctg aaaaggcagc agctgataag
     5941 aaagcagcgg cagaaaaagc cgccgcagac aaaaaagcgg cagctgcaaa agcagcagct
     6001 gaaaaagccg ctgcagcaaa agctgccgca gaggcagatg atattttcgg tgagctaagc
     6061 tctggtaaga atgcaccgaa aacgggggga ggggcgaaag ggaacaatgc ttcgccagcc
     6121 gggagtggta atactaaaaa caatggcgca tcaggggccg atatcaataa ctatgccggg
     6181 cagattaaat ctgctatcga aagtaagttc tatgacgcat cgtcctatgc aggcaaaacc
     6241 tgtacgctgc gcataaaact ggcacccgat ggtatgttac tggatatcaa acctgaaggt
     6301 ggcgatcccg cactttgtca ggctgcgttg gcagcagcta aacttgcgaa gatcccgaaa
     6361 ccaccaagcc aggcagtata tgaagtgttc aaaaacgcgc cattggactt caaaccgtaa
     6421 tcgcgatgct gactgttccg acggtcaaca tcaggcaccg gttgccacgg ggttctggta
     6481 gttttgtgta ttttagtttg ttaacattct gctaaattat cgtgggccat cggtccagat
     6541 aagggagata tgatgaagca ggcattacga gtagcatttg gttttctcat actgtgggca
     6601 tcagttctgc atgctgaagt ccgcattgtg atcgacagcg gtgtagattc cggtcgtcct
     6661 attggcgttg ttcctttcca gtgggcgggt cctggtgcag cacctgaaga tattggcggc
     6721 atcgttgctg ccgacttgcg taacagcggt aaatttaatc cgttagatcg tgctcgtctg
     6781 ccacagcagc cgggtagtgc gcaggaagta caaccagctg catggtctgc gctggggatc
     6841 gacgcagtgg ttgtcggcca ggtcactccg aatccggacg gctcttacaa tgttgcttat
     6901 caacttgttg acactggcgg cgcaccgggt actgtacttg ctcagaactc gtacaaagtg
     6961 aacaagcagt ggctgcgtta tgctggtcat accgccagtg acgaagtatt tgaaaaactg
     7021 accggcatta aaggtgcttt ccgtacccgt attgcctacg ttgttcagac caacggcggt
     7081 cagtttccgt atgaactgcg cgtatctgac tatgacggtt acaaccaatt tgtcgttcac
     7141 cgttcaccgc agccgctgat gtctccggcg tggtcaccag acggttctaa actggcttat
     7201 gtgaccttcg aaagcggtcg ttccgcgctt gtcatccaaa cgctggcaaa tggcgctgta
     7261 cgtcaggtgg cttcattccc gcgtcacaac ggtgcacctg cattctcgcc agacggcagc
     7321 aaactggcat tcgccttgtc gaaaaccggt agcctgaacc tgtacgtaat ggatttggct
     7381 tcgggtcaga tccgccaggt gacggatggt cgcagtaaca ataccgaacc gacctggttc
     7441 ccggacagcc agaatctggc atttacttct gaccaggccg gtcgtccgca ggtttataaa
     7501 gtgaatatca acggcggtgc gccacaacgt attacctggg aaggttcgca gaaccaggat
     7561 gcggatgtca gcagcgacgg taaatttatg gtaatggtca gctccaatgg tgggcagcag
     7621 cacattgcca aacaagatct ggcaacggga ggcgtacaag ttctgtcgtc cacgttcctg
     7681 gatgaaacgc caagtctggc acctaacggc actatggtaa tctacagctc ttctcagggg
     7741 atgggatccg tgctgaattt ggtttctaca gatgggcgtt tcaaagcgcg tcttccggca
     7801 actgatggac aggtcaaatt ccctgcctgg tcgccgtatc tgtgataata attaattgaa
     7861 tagtaaagga atcattgaaa tgcaactgaa caaagtgctg aaagggctga tgattgctct
     7921 gcctgttatg gcaattgcgg catgttcttc caacaagaac gccagcaatg acggcagcga
     7981 aggcatgctg ggtgccggca ctggtatgga tgcgaacggc ggcaacggca acatgtcttc
     8041 cgaagagcag gctcgtctgc aaatgcaaca gctgcagcag aacaacatcg tttacttcga
     8101 tctggacaag tacgatatcc gttctgactt cgctcaaatg ctggatgcac acgcaaactt
     8161 cctgcgtagc aacccgtctt acaaagtcac cgtagaaggt cacgcggacg aacgtggtac
     8221 tccggaatac aacatctccc tgggtgaacg tcgtgcgaac gccgttaaga tgtacctgca
     8281 gggtaaaggc gtttctgcag accagatctc catcgtttct tacggtaaag aaaaacctgc
     8341 agtactgggt catgacgaag cggcatactc caaaaaccgt cgtgcggtac tggtttacta
     8401 agagaattgc atgagcagta acttcagaca tcaactattg agtctgtcgt tactggttgg
     8461 tatagcggcc ccctgggccg cttatgctca ggcaccaatc agtagtgtcg gctcaggctc
     8521 ggtcgaagac cgcgtcactc aacttgagcg tatttcaaat gctcacagcc agcttttaac
     8581 ccaactccag caacaactct ctgataatca atccgatatt gattccctgc gtggtcagat
     8641 tcaggaaaat cagtatcaac tgaatcaggt cgtggagcgg caaaagcaga tccttttgca
     8701 gatcgatagc ctcagcagcg gtggtgcagc ggcgcaatca accagcggcg atcaaagcgg
     8761 tgcggcggca tcaacgacgc cgacagctga tgctggtact gcgaatgctg gcgcgccggt
     8821 gaaaagcggt gatgcaaaca cggattacaa tgcagctatt gcgctggtgc aggataaatc
     8881 ccgccaggat gacgcaatgg tggcatttca gaatttcatc aaaaattacc ctgattcaac
     8941 ttacctgcca aacgccaatt attggctggg tcagttaaac tacaacaagg gtaaaaaaga
     9001 tgatgcggcg tattattttg cttcggtagt gaaaaactat ccgaagtcac cgaaggctgc
     9061 agatgcgatg tttaaagtcg gcgtcatcat gcaggacaaa ggtgacaccg caaaagcgaa
     9121 agccgtatac cagcaggtta tcagtaaata ccctggtact gatggcgcta aacaggcgca
     9181 aaaacgtctg aacgcgatgt aatgcataac acacgaccag aagtcgcatt atttctggtc
     9241 gtgtcgtgcg aatcataagc agttgagtga tctgcatcga aatttttgtt gcgctcaagt
     9301 ctgaaatcag taatatatgc cgccgttgcc acgggatatc aaacaaaccg aaagcaacga
     9361 aaaagtgggt cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt
     9421 tcgaatcctg cacgacccac caatcgctaa ggtggaagcg gtagtaaaac gtgaaggata
     9481 acgttgcgtc agcaacggcc cgaagggcga gacgaagtcg agtcatcctg cacgacccac
     9541 cactaacata gttagttgta gtatccagcg cagtatcggg tgattagctc agctgggaga
     9601 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccaccgggtc
     9661 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
     9721 acgacccacc actaattacg gtgggttcgg tggaagtagt ttgtagtatc cagcgcagta
     9781 tcgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
     9841 tcccgtcatc acccaccact cgggtcgtta gctcagttgg tagagcagtt gacttttaat
     9901 caattggtcg caggttcgaa tcctgcacga cccaccaatt ttaacatcaa actcagatgt
     9961 taagcgtgaa ggataacgtt gcgccagcaa cggcccgaag ggcgagcgaa gcgagtcatc
    10021 ctgcacgacc caccaatttt aaagattggt tccgagtaga aatctttcag gtaacacccg
    10081 tatgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
    10141 aatcctgcac gacccaccat cctgaatgat taaagcagca taatcccgca aggggtcgtt
    10201 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    10261 acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctatctgcga tactcaaaga
    10321 ttcgaacctg cagcaggttc gagttgagcg cagcgaaaca acggagccgc tcgcggcgac
    10381 ggcccgaagg gcgagcgaag cgagtcatcc tgcacgaccc accaatgtaa aaaagcgccc
    10441 taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt
    10501 tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag
    10561 tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctattcag
    10621 gcatctctca atttcaattc ttaaccctga tgacatactc cgcgcttgct gcgtaaacaa
    10681 ctctacgtcg gaggaataag acgcaaagtg tggcattcgt catctcatac gtaattcgag
    10741 tgacttttcc ccaccattcg actatcttgt ttagcatata aaacaaatta cgccgataat
    10801 agcgaatatc acgcttaagt cggttttaac gttaagcctg tgaaacgaga tggtaagatg
    10861 agcgtgatgt ttgatccaga cacggcgatt tatcctttcc ccccgaagcc gacgccgtta
    10921 agcattgatg aaaaagcgta ttaccgcgag aagataaaac gtctgctaaa agaacgtaat
    10981 gcggtgatgg ttgcccatta ctataccgat cccgaaattc aacaactggc agaagaaacc
    11041 ggtggctgta tttctgattc tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct
    11101 acgttgttag tcgctggggt gagatttatg ggagaaaccg ccaaaattct cagttcggaa
    11161 aaaacaattc tgatgccgac acttcaggct gaatgttcac tggatctcgg ctgccctgtt
    11221 gaagaattta acgcgttttg cgatgcccat cccgatcata ctgtcgtcgt ctacgccaac
    11281 acttctactg cggtaaaagc gcgggcagat tgggtggtaa cttcaagcat tgccgtcgaa
    11341 cttattgatc atctggatag tttgggtgaa aaaatcatct gggcacccga caaacatctg
    11401 gggcgttacg tgcaaaaaca gacgggcgca gacattttat gctggcaggg tgcctgcatt
    11461 gtgcatgatg aatttaagac tcaggcgtta acccgcttgc aagaagaata cccggatgct
    11521 gccatactgg tgcatccaga atcaccgcaa gctattgtag agatggcgga tgcggtcggt
    11581 tccaccagtc aactgatcgc tgctgcgaaa acattgccac atcagcgact tatcgtggca
    11641 accgatcggg gtattttcta caaaatgcag caggcggtac cagataaaga gttactggaa
    11701 gcaccaaccg cgggtgaggg cgcaacctgc cgcagctgcg cgcattgtcc gtggatggcc
    11761 atgaatggcc ttcaggccat cgcagaggca ttagaacagg aaggaagcaa tcacgaggtt
    11821 catgttgagg aaaggctgcg agagagggcg ctggtgccgc tcaatcgtat gctggatttt
    11881 gcggctacac tacgtggata acgaataata aggcgtaacg ttacgctttg ggggaaagat
    11941 ggattttttt agtgtgcaga atatcctggt acatatacca ataggagcag gcggttatga
    12001 tctctcatgg atcgaagcgg taggcacgat cgccgggttg ctgtgtattg gccttgccag
    12061 tctggagaag atcagcaact acttctttgg cctgatcaac gtcacattgt ttggcattat
    12121 tttctttcag attctgctgt atgccagcct gctattacag gtgtttttct ttgccgcgaa
    12181 tatttacggc tggtatgcgt ggtcgcgaca aaccagtcag aacgaggcgg agttgaaaat
    12241 tcgctggttg ccattgccga aggcgctaag ctggttggcg gtttgcgttg tttcgattgg
    12301 tctgatgacg gtatttatca atccggtgtt tgcatttttg acccgcgtgg cagtcatgat
    12361 catgcaagca ttaggattac aggttgcgat gcctgaacta cagccggacg ctttcccgtt
    12421 ctgggattcc tgcatgatgg tgttatctat cgtggcaatg attctgatga cgcgtaagta
    12481 tgtggaaaac tggctgttgt gggtgattat taacgtgatt agcgtcgtta tttttgcact
    12541 tcagggcgtt tacgccatgt ctctggagta catcatcctg acctttattg ccctcaacgg
    12601 cagccggatg tggatcaaca gcgcacgcga aagaggttca cgcgcgctgt cccattaatg
    12661 gtgatgatgt gaatggccag acaccccttc attcaggtgg cagtctggcc catgacaagg
    12721 ttgatattcc atctgaatag tggcgtgttc aatctgatag tgatccatca ggtagtgttg
    12781 gatctgatct aacaaggcat cgtgatcgtg tggcgggatc acctgcacat gcagcgtcat
    12841 caccggcttc tcgcctacca tccatacatg tacatggtgc acattgcgga cttccgggat
    12901 ttcccggcac atacgacgct tcagttcggc gatatccagc gataccggtg caccttcaag
    12961 taattcattc acgctatctt tcaacagtcg ccacgcgcta cgcagaacca gaagcgacac
    13021 cagtattgag agaatggggt cagcaggtgt ccagccggtc cagataataa tcaacgcggc
    13081 aataatagcc ccaaccgaac ccagcaggtc cccgagcaca tgcagtgccg ctgctcgcac
    13141 gttgaggttt ttctcttcgc tgccgtgatg aagtaaccag aaagaaagta tatttgccag
    13201 cagcccagcc acggcaattg ccatcatcat gccgccctcg accggacgcg gagtgcggaa
    13261 gcgttctatc gcctcccaga caatcaaaat agtaatcacc accagggcga tggcattcac
    13321 aaacgccgcg agcgtggtca atcttagcca accgaaagtg tggcgaatgg tgggaggacg
    13381 acgggaaaat tgcactgcga gtagggcgaa aagcagggcg gcagtatcgg tcaacatatg
    13441 acccgcatcg gccagcaatg ccagagaacc ggaaagaaaa ccaccaatga cttctacgag
    13501 cataaacccg gcagtcacgc cgaaagcata caacaagcga cgagcattat tatcttctgg
    13561 caggtgtgag gaagtgtgtg agtgtgagtg cgccatgacg agtcatccct taattattct
    13621 cacttaactt tatgacatca tactgctttt agaagggaaa aattaaaagg gagagactcc
    13681 gctctcccat tattggctat ttcgcagggt tactgcgtgg taccgtcggt tttggtatcg
    13741 acatcattgt tgatgccatc accagtttgt acctttttat tgatatccgg acagcgacca
    13801 tctttgcaca tggtgttctt gtgctcttca tctttggtca tgccgtcatt gttcattgaa
    13861 gaaccatccg gatgcagcat tgtgccgcca gaaccggtat ttaccccgtt attgtcgacg
    13921 ttatttggcg cgacattttc acgggcatca ggggctacct ggcccgcatc agctgcggcg
    13981 tttgcctggc cgttattgct ttgcgctccg ctatcggcag ccagtgcggc accgctggca
    14041 aggcttagag tggcagtcaa aaatagtgtg gccagttttg tcattttcat aggatgctcc
    14101 tgttatggtc gttatgtcgg ataacctctt ccaacagtgc atttgcaggt gaatataagg
    14161 caatggttta agatttcagc caggctaaga tacccagcag agaatcttga aataattaac
    14221 aaacaaagga gttacagtta gaaattgtag gagagatctc gtttttcgcg acaatctggc
    14281 gtttttcttg ctaattccag gattaatccg ttcatagtgt aaaaccccgt ttacacattc
    14341 tgacggaaga tatagattgg aagtattgca ttcactaaga taagtatggc aacactggaa
    14401 cagacatgaa ttatcagaac gacgatttac gcatcaaaga aatcaaagag ttacttcctc
    14461 ctgtcgcatt gctggaaaaa ttccccgcta ctgaaaatgc cgcgaatacg gtcgcccatg
    14521 cccgaaaagc gatccataaa atcctgaaag gtaatgatga tcgcctgttg gtggtgattg
    14581 gtccgtgctc aattcatgat cctgtcgcgg ctaaagagta tgccgctcgc ttgctgacgc
    14641 tgcgtgaaga gctgcaagat gagctggaaa tcgtgatgcg cgtctatttt gaaaagccgc
    14701 gtactacagt gggctggaaa gggctgatta acgatccgca tatggataac agcttccaga
    14761 tcaacgacgg tctgcgtatt gcccgcaaat tgctgctcga tattaacgac agcggtctgc
    14821 cagcggcggg tgaattcctg gatatgatca ccccacaata tctcgctgac ctgatgagct
    14881 ggggcgcaat tggcgcacgt actacggaat cgcaggtgca ccgcgaactg gcatcagggc
    14941 tttcttgccc ggtaggcttc aaaaatggca ctgatggtac gattaaagtg gctatcgatg
    15001 ccattaatgc cgccggtgcg ccgcactgct tcctgtccgt aacgaaatgg gggcattcgg
    15061 cgattgtgaa taccagcggt aacggcgatt gccatatcat tctgcgcggc ggtaaagagc
    15121 ctaactacag tgcgaagcac gttgctgaag tgaaagaagg gctgaacaaa gcaggcctgc
    15181 cagcacaggt gatgatcgat ttcagccatg ctaattcgtc caaacaattc aaaaagcaga
    15241 tggatgtttg tgctgacgtt tgccagcaga ttgccggtgg cgaaaaggcc attattggcg
    15301 tgatggtgga aagccatctg gtggaaggca atcagagcct cgagagcggg gagccgctgg
    15361 cctacggaaa gagcatcact gatgcctgca tcggctggga agataccgat gctctattac
    15421 gtcacctggc gaatgcagta aaagcgcgtc gcgggtaagg tttaattgtc ggatgcgccg
    15481 tcagagtggc gtatccgatg aatcaccgta gccctgacaa gacgcgtagc gtcgcatcag
    15541 gcaatgtgct ccattgttag caacgacaaa caaaaaagcc gactcacttg cagtcggctt
    15601 tctcattttt aagcgaatga cgtttacttc gctttaccct ggttcgcaac ggccgctgct
    15661 ttcgccgcga tctcgtcagc attacccaga taatagcgtt tcaacggttt gaaattctcg
    15721 tcgaactcat acaccagtgg cacaccagtc gggatattca gctcaagaat ctcttcttcg
    15781 ctcatgttat cgagatattt caccagcgca cgtaacgagt taccgtgtgc ggcgatgatt
    15841 acgcgctcac cgctcttcat acgcggcaga atggtttcat tccagtaagg gatcacgcgg
    15901 tcaatggtca gcgccaggct ttccgtcagc ggcagttctt tctcgctcag tttcgcgtaa
    15961 cgcggatcgt gacccggata acgctcatca tctttagtca gttccggcgg agtcactgca
    16021 aaaccacgac gccactgttt cacctgctcg tcgccatact tttcagcagt ttccgctttg
    16081 ttcagaccct gcaacgcacc gtagtgacgt tcgttcagtt tccaggattt ctcaacgggc
    16141 agccatgcct gatccagttc gtccagcaca ttccacaggg tatgaatagc gcgtttcagc
    16201 acagaggtgt aagcaaagtc aaagctgtaa ccttcctctt tcagcagctt acctgctgct
    16261 tttgcttcgc ttacgccttt ctcagacaga tccacgtcgt accaaccggt gaaacggttt
    16321 tctttgttcc actgactttc gccatgacga accagaacca gcttagttac agccatatac
    16381 ttactcctca aatcatcttt taatgataat aattctcatt atattgccgt gacgaagcaa
    16441 cagcaatgct tacgcataac catagcgaaa atagtgtcgc agtgtaaggt tgttgtgaat
    16501 attgagttgc aaatatgtcg gtgtttgctg gtgatttgaa caatatgagg ataaagccct
    16561 cagtacgagg gcttaacatt actcagcaat aaactgatat tccgtcaggc tggaatactc
    16621 ttcgccagga cgcaggaagc agtccggttg cggccattca gggtggttcg ggctgtccgg
    16681 cagaaactcg ctttccagag ctagcccttg ccagtcggcg taaggttcgg ttccccgcga
    16741 cggtgtgccg ccgaggtagt tgccggagta gaattgcaga gccggagcgg tggtgtagac
    16801 cttcagctgc aatttttcat ctgctgacca gacatgcgcc gccactttct tgccatcgcc
    16861 tttggcctgt aacaagaatg cgtgatcgta acctttcact ttgcgctgat cgtcgtcggc
    16921 aagaaactca ctggcgatga ttttggcgct gcggaaatca aaagacgttc cggcgacaga
    16981 tttcaggccg tcgagcggaa tgccgccttc atcaaccggc agatattcgt ccgccagaat
    17041 ctgcaacttg tgattgcgca cgtcagactg ctcgccgtca agattgaaat agacgtgatt
    17101 agtcatattc accgggcaag gtttatcaac tgtggcgcga taagtaatgg agatacggtt
    17161 atcgtcggtc agacgatatt gcaccgtcgc accgagatta cccgggaagc cctgatcgcc
    17221 atcatctgaa ctcagggcaa acagtacctg acgatcgttc tggttcacaa tctgccagcg
    17281 acgtttgtcg aacccttccg ggccgccgtg cagctggtta acgccctgac ttggcgaaag
    17341 cgtcacggtt tcaccgtcaa aggtataacg gctattggcg atacggttgg cataacgacc
    17401 aatagaggcc cccagaaacg cggcctgatc ctgatagcat tccgggctgg cacagccgag
    17461 cagcgcctcg cggacgctgc catcggaaag cggaatacgg gctgaaagta aagtcgcacc
    17521 ccagtccatc agcgtgacta ccatccctgc gttgttacgc aaagttaaca gtcggtacgg
    17581 ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt
    17641 ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacagct
    17701 tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg
    17761 ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga
    17821 gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc
    17881 atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg
    17941 cgggcgtttt cagtcaggat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc
    18001 gcaacagcgt tgaactcttc aatggtgaca tcgcgcaggg ctggctgctg gaagaaacgc
    18061 gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg
    18121 aagttactgt taatgatgac gacagccaca cctttcggca tggaaactgc tttagtcccc
    18181 agtgagcggc agtcaatcag caaggcatga tctttcttgc cgagtgcgga aattagctga
    18241 tccatgatcc cgcagttaca gccaacaaac tggttttctg cttcttgacc gttaagcgcg
    18301 atttgtgcgc catccagcgg caaatgataa agctgctgca atacagttcc gaccgcgact
    18361 tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgct gatcaccatg
    18421 tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag
    18481 ttagcccatt gatagttttc atgcgcgaca atgggcgcat cgagggaaaa ctcgtcgagc
    18541 tgattttcat aatcggctgc cattacgcga actttatggt catcgcgtgg cgcacagctg
    18601 atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt
    18661 tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat
    18721 gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg
    18781 cgaaaatgga tatcgctgac tgcgcgcaaa cgctctgctg cctgttctgc ggtcaggtct
    18841 cgctgggtct ctgccagcat ttcataacca accataaatt tacgtacggt ggcggagcgc
    18901 aacagaggcg gataaaagtg cgcgtgcagc tgccagtgtt gattctcttc gccattaaat
    18961 ggcgcgccgt gccagcccat agagtagggg aaggagcact ggaagaggtt gtcataacga
    19021 ctggtcagct ttttcaacgc cagtgccaaa tcgctgcgct gggcgtcggt caaatcggtg
    19081 atccgtaaaa cgtgggcttt gggcagcagt agcgtttcga acggccaggc agcccagtaa
    19141 ggcacgacgg ctaaccagtg ttcggtttcg acaacggtac ggctaccgtc tgccagctcg
    19201 cgctgaacat aatccaccag cattggcgat ttctgctcgg caaaatattc tttttgcagg
    19261 cggtcttcgc gctcagcttc gttaggcagg aagctatttg cccaaacctg tccgtgcgga
    19321 tgcgggttag agcagcccat cgccgcacct ttgttttcaa agacctgcac ccacgggtac
    19381 gtttttccca gctctgcggt ttgctcctgc caggttttga cgatttccgt caatgccgca
    19441 acgctcagtt ctggcaacgt tttactgtga tccggtgaaa agcagatcac ccggctggta
    19501 ccgcgcgcgc tctggcaacg catcagcgga tcgttgcttt ctggcgcatc tggcgtgtca
    19561 gacatcaagg ccgcaaaatc attagtgaaa acgtaagtcc cggtgtaatc ggggttttta
    19621 tcgccagtca cccgcacatt acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac
    19681 acctgtttgg ctggcgtttc ctgcgccccc tgccaggggc gcttagcgcg gtgcggtgaa
    19741 accagaatcc attgcccggt gagcgggttg tagcggcgat gtggatgatc aacgggatta
    19801 aattgcgtca tggtcgttcc ttaatcggga tatccctgtg gatggcgtga ctgccagtgc
    19861 caggtgtcct gcgccatttc atcgagtgtg cgcgtcacgc gccagttcag ttcacggtcg
    19921 gctttgctgg cgtccgccca gtaggccgga aggtcgccct cgcgacgcgg tgcaaaatga
    19981 tagttaactg gtttgccgca ggctttgctg aaggcattaa ccacgtccag cacgctgctg
    20041 cctacgccag cgccgaggtt gtagatgtgt acgcctggct tgttcgccag tttctccatc
    20101 gccacgacgt gaccgtccgc cagatccatt acgtggatgt aatcgcgtac gccagtaccg
    20161 tcttcggtcg gataatcgtt accaaaaatc gccagcgagt cgcgacggcc tacagcaacc
    20221 tgggcgatgt atggcatcag gttattcgga atgccttgcg gatcttcgcc catatcgccc
    20281 gacggatgtg cgccaaccgg gttgaagtag cgcagcaggg caatgctcca gtccggctgg
    20341 gctttttgca gatcggtgag gatctgttca accatcagtt tgcttttgcc gtaagggctt
    20401 tgcggtgtgc cggtcgggaa gctttcaacg tatggaattt tgggctgatc gccataaacg
    20461 gtggcggagg agctaaagat aaagtttttg acgttcgcgg cgcgcatggc gctaatcagg
    20521 cgcagggtgc cgttaacatt gttgtcgtaa tattccagcg gtttttgtac cgattcgcca
    20581 acggctttca gcccggcgaa gtggatcacg gtgtcgatag cgtgatcgtg caggatctcg
    20641 gtcatcaacg cttcgttacg gatatcgcct tcaacaaacg ttggatgttt gccgcctaaa
    20701 cgctcgataa cgggcagtac gctgcgctta ctgttacaga ggttatcaag aatgatgaca
    20761 tcatgaccgt tttgcagtaa ttgcacacag gtatgacttc caatgtaacc gctaccaccg
    20821 gtaaccagaa ctctcataat tcgctccatt aggcttatgg tatgaaataa ccatagcata
    20881 acaaagatgc gaaaagtgtg acatggaata aattagtgga atcgtttaca caagaattta
    20941 gccgtttttt atgcgcgatt aagtgattat aaaacagagg gtttatgaat gattgcgctt
    21001 tttatctgaa aaaaaagacg cggtttcatg cctggatgcg tcgaaccgtt ggccggagag
    21061 ggcgcttagg ccgcctacgg cacggtcagc actaccgact caatttattt ttgtcagcac
    21121 atagcgatag agtccgccgt ctggcacgaa ctcaagacga tgggtaatac aggcaggcgc
    21181 atcttcagcg tggtgcgaaa caaacaacaa ttgcgtttca ccttcgctaa tcagcacatc
    21241 aacaaaccgg cggataagct ggcgattgag cggatcaagc ccctgtagtg gttcatcgag
    21301 aataagcaac gtcggatgtt tcaccagtgc acggacgatc agcgccaggc gttgttgccc
    21361 ccaggaaaga ctatggaacg gagcgtcagc cgtgcgttta tcaatgccga gaatatccag
    21421 ccactgctgc accagttttt gctggcgatc cgaaacggcc tgataaatgc caatcgaatc
    21481 aaaatagcca gaaaggatca cattacgcac ggtagtgctg acccggtaat ccagatgcaa
    21541 actactgctg acgtaaccga tatgcttttt gatatcccag atggtttcgc cgctgccgcg
    21601 acgtcgtccg aaaagcgtca aatcgttgct gtaaccttgc ggatgatcgc cagtaatcag
    21661 gcttaataac gtcgattttc ccgcgccatt tggcccgaca atttgccagt gctcgcctgg
    21721 attcacctgc cagctaaggt tattaagaat ggggcgatcg ttataagaaa ccacgccgtt
    21781 gttcagcaca atgcgcggtt cgttggcggg taaggcgtga cgtgctgaag gttcatccgg
    21841 ctccggcagt tgtacacctt caagctgttc actatgcgcc agttgcgcga cgagtgcttg
    21901 ttggagcagt tcctctttag cgctagtttc cgctaacgtg caatccgcca gcacgccagc
    21961 aaactggaca aactccggga tctcatcgaa gcgattgagc accagtacca gagtaatacc
    22021 ggactgatgt aacgaggcga gtagctcagc cagctgctga cgtgaggcaa catccaggcc
    22081 atcgaacggt tcatcaagga tcaacaagtc aggctccgac atcagcgcct ggcacagcag
    22141 ggtttttcgc gtctcgccag tggaaaggta tttaaagcgt cggtcgagga gggcggtaat
    22201 accgaactgc tgcgccagtt gcatgcaacg cggagtatcc tttacttcat cctgaatgat
    22261 ctcagccgtg gtacgcccgg tgtcatcttc gccaggacta agcatatcgg tgttattccg
    22321 ctgccattcg tcgctgacga gcttttgcaa ttgctcgaag gagagacgag tgatgtggga
    22381 aaactggctt tgccgttcac ctttcaaaag cggaagttcc cccgccagcg cacgggccag
    22441 ggccgatttc ccgcttccat tcgaaccgac aaacgcccaa ctatcacccg cgtttaacgt
    22501 tagctgaggc aattgcagcg tttttgtgtc gctaagacga aacgtgcctt gcaaaatttg
    22561 caacgatgac attttatatc ccattttgtg cagcgattac tgacagggat acgtgtttca
    22621 taacaaattg tcaacacgct tagcacagcg tggcgataat cacgctgtcg gcattaaagt
    22681 aggccgtgac attctgtcct tgctgaagag aagtcgcttc atttaccggc actgtggcgc
    22741 acagtgtttg cccgtcgggt agcgccatta atacttcgca ctgctctgcg ccgcgctcaa
    22801 tatgactaat aatacccggt aattggttgt cggcgttttg tgcgaccgct tcgtcctgag
    22861 taatgcctac ccacggcgct tttagtaata tcaacacttc tttgccttca tccagcccca
    22921 gacgcgcgcc gctttgtgcg gtaattgcga ctttcaggcg tgtttttccg tcagccagta
    22981 agacatcaac atgctgttga acgtcatcat gatcgcgggc ggtgatggtg ccaaaccatt
    23041 ggttacgggc gctggtttgc agtgaaaaac gtgagatcgc ggccagcagg ctgttcagcg
    23101 gcagggcgtc atcgtcactt aacacatcaa aggctttttg ctggatttgc gccagtaagt
    23161 catagagctg aatcagtcgc tgaccatagc gggtcagtac tgcgccgcct ccacctttac
    23221 cgcctgttgc gcgctcaacc agaatatgct cacttaactg attcatctcg ttaatggcat
    23281 cccaggcgct tttatagctg ataccggcat ctttcgctcc ctggctaatg gaaccggaaa
    23341 gcgcaatgtg ttttagtagc gaaatgcggc gcgggtcggc gaataatttt tgttggagct
    23401 taagggtgag aaggatttcg gcctgcataa caatgtcctg gcaaaagtct tattgtgacg
    23461 gaaaacgaac gccacgcaaa gctgaccgca caaaagggga gtgcttttct gtgcttagcg
    23521 gttagaatag tctcatgact atatctggag ttgaccatgt tagagttatt aaaaagtctg
    23581 gtattcgccg taatcatggt acctgtcgtg atggccatca tcctgggtct gatttacggt
    23641 cttggtgaag tattcaacat cttttctggt gttggtaaaa aagaccagcc cggacaaaat
    23701 cattgattcc ctgaatgccc gcttagtcgg gcattttctt tttctcaact tcctgctttt
    23761 cctgccgata ttttttctca tctacctcac aaaggttagc aataactgct gggaaaattc
    23821 cgaacttgcc gttatattga cgcctacata acgttacatt aaggggttac caatggctcg
    23881 taaatggttg aacttgtttg ccggggcggc actctctttc gctgttgctg gcaatgcact
    23941 ggcagatgaa gggaaaatca cggtgttcgc cgccgcatca ctgactaacg caatgcagga
    24001 cattgctacg cagtataaaa aagagaaagg cgtggatgtg gtttcttctt tcgcttcgtc
    24061 atctactctc gcccgtcaga ttgaagcggg agcccctgcg gatctgttta tttctgccga
    24121 tcagaaatgg atggattatg cggttgataa aaaagcgatc gatacagcta cgcgtcagac
    24181 actgctcggc aatagcctgg tcgttgtagc accgaaagcc agcgagcaga aagatttcac
    24241 catcgacagc aaaaccaact ggacttcact gctgaatggc ggtcgcctgg cggttggcga
    24301 tccggaacat gttcccgctg gcatttatgc aaaagaagca ctgcaaaaac tgggcgcatg
    24361 ggatacgctc tccccgaaac tggctccggc ggaagatgtt cgtggcgcgc tggcgttggt
    24421 cgaacgcaat gaagcgccgc tgggtattgt ttacggttct gacgcagttg ccagcaaagg
    24481 ggtaaaagtg gttgccacct tcccggaaga ttcacataaa aaagtggaat acccggttgc
    24541 tgttgtggaa gggcataaca atgcgacagt gaaagcattt tatgattatc tgaaggggcc
    24601 gcaggctgcg gaaatcttta aacgttacgg atttacaacc aagtaatgat actgaccgat
    24661 ccagaatggc aggcggtttt actaagcctg aaagtttctt ccctggctgt gctgtttagc
    24721 ctgccgtttg ggatcttttt tgcctggtta ctggtgcgtt gcacgtttcc gggcaaagct
    24781 ctgctcgata gcgtactgca tctaccgctg gtgttaccgc ccgtggtcgt cggttactta
    24841 ttattagttt cgatgggacg gcgcggattt atcggtgaac gtctgtatga ctggtttggc
    24901 attaccttcg cctttagctg gcgcggcgcg gttctcgctg ccgccgtcat gtcgtttccg
    24961 ctgatggtgc gggcaattcg tctggcgctg gaaggggttg atgtcaaact ggaacaggcc
    25021 gcaagaacac tgggtgccgg gcgctggcgc gttttcttta ctatcacgtt accgctgacc
    25081 ttaccgggca ttattgttgg tacggtactg gcttttgctc gttctctcgg tgagtttggt
    25141 gcaaccatca cctttgtgtc gaacattcct ggtgaaacgc gaaccattcc ttctgccatg
    25201 tataccctga tccagacccc cggcggggaa agtggcgcgg cgagactgtg cattatttct
    25261 attgcactgg cgatgatctc cctgttgatt tcagaatggc tggccagaat cagccgtgaa
    25321 cgggcggggc gctaatcatg ctggaactga atttttccca gacgttgggc aaccattgcc
    25381 tgaccattaa tgaaacgctg cccgccaatg gtatcactgc tatctttggc gtctccggtg
    25441 ccggaaaaac ttcgctgatt aacgccatca gtggactgac gcgcccgcaa aaagggcgga
    25501 ttgtcctcaa tgggcgggta ctaaatgatg ccgaaaaagg tatctgcctg acgccggaaa
    25561 agcgtcgcgt tggctatgtt tttcaggatg cgcggctgtt cccgcattac aaagtgcgtg
    25621 gcaatctgcg ctacggcatg tcgaaaagta tggtcgatca gttcgataag ctggtggcgc
    25681 ttttaggcat tgaaccgttg cttgaccgtt taccaggcag cctgtccgga ggcgaaaaac
    25741 agcgcgtggc gattggtcgg gctttgctga cagcaccgga attgctgttg ctggatgaac
    25801 cgctggcgtc actggatatt ccgcgtaaac gcgaactgtt gccttatctg caacggctga
    25861 cacgggaaat caacattccg atgttgtatg tcagccattc gctggatgag atcctccatc
    25921 tggcagacag agtgatggta ctggaaaacg gtcaggtgaa agcctttggc gtgctggagg
    25981 aagtgtgggg cagtagcgtg atgaatccgt ggctgccgaa agagcaacaa agtagcattc
    26041 tgaaagtgac ggtgcttgag catcatccgc attacgcgat gaccgcgctg gcgctgggcg
    26101 atcagcattt gtgggtcaat aagctggacg aaccgctgca agctgcgcta cgcatccgca
    26161 ttcaggcttc agatgtttct ctggttttac aaccgccgca gcaaaccagc attcgtaacg
    26221 tattgcgggc aaaagttgtt aatagttatg acgacaacgg ccaggtggaa gtggagctgg
    26281 aagtcggcgg taaaacgctg tgggcgcgta tcagcccgtg ggccagggat gaactggcga
    26341 tcaaaccagg cctgtggctg tacgcgcaaa ttaaaagtgt gtcgataacc gcctgattaa
    26401 atcaggtggc tataaatgaa ctgggcaatg ctgtcggtgg tgttatcacc aatcacaatg
    26461 ttggcgcgcg cttttaccgc gtcatcggcg ttgcccatcg ccacgcctgt accagcggct
    26521 tccagcatac tgatatcatt aaagttatcg ccgaatgcca cgacgttttc catcgaccaa
    26581 ccttgtgcct caacccattt cgtcaaacgt ttacctttgc tgttgccgcc gcgtgcaata
    26641 tcaacctgat cgtgccagga ccattcacac tcaagtccca gttcatgttc gacatgctta
    26701 ccaaaatgct gcaattgcgg caggtcatcg tgcgtcaggg cgaacttcca tacggcgtta
    26761 acttgttgcg ccgtttcagc cagagaagcg acttgtgtga aagtcggacg ctgttccggt
    26821 ggcagggttt gcgcccagtt agacgtgcga atgacatgcc cggtcgggtg ctcatagacc
    26881 attgcatcat cgacatacat cagaccgtga atgtggtgtt cattcagcat ctcaatgagt
    26941 tgcagggctt tattaacggg cattgggtcc gcttccagca ctgtttttgc atgataatca
    27001 tacaaatagg tgccattaca gcaaatagca ggtgtatcca gcgccagcgc ctgataaaaa
    27061 ggatgaatag cgacgtgatg gcgacctgtg acgatgatta attgatagcc tgcttcgcaa
    27121 gcgcgggcca gggcttctat cgatgatgga agcaaggtct ttttcggggt caataaggtg
    27181 ccgtctaagt cgagagcaat cacgcgtgtg gtcatggcgt attccagatt aaggttaaga
    27241 attttcgtct gcgcgaatgg tacaccgata ccactatcgg acaaaattct gcgttttaat
    27301 tcagcattca ccgccaaaag cgactaattt tagctgttac agtcagttgc taaatgcaaa
    27361 ggagcattca tgaagcaaac agtttatatc gccagccctg agagccagca aattcacgtc
    27421 tggaacctga atcatgaagg cgcactgacg ctgacacagg ttgtcgatgt gccggggcag
    27481 gtgcagccga tggtggtcag cccggacaaa cgttatctct atgttggtgt gcgccctgag
    27541 tttcgcgtcc tggcgtatcg tatcgccccg gacgatggcg cactgacctt tgccgcagag
    27601 tctgcgctgc cgggtagtcc gacgcatatt tccaccgatc accaggggca gtttgtcttt
    27661 gtaggttctt acaatgcggg taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg
    27721 ggcgtcgtcg atgtggtcga ggggctggac ggttgccatt ccgccaatat ctcaccggac
    27781 aaccgtacgc tgtgggttcc ggcattaaag caggatcgca tttgtctgtt tacggtcagc
    27841 gatgatggtc atctggtggc acaggaccct gcggaagtga ctaccgttga aggggccggc
    27901 ccgcgtcata tggtattcca tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac
    27961 agctcagtgg atgtctggga actgaaagat ccgcatggca atatcgaatg tgtccagacg
    28021 ctggatatga tgccggaaaa cttctccgac acccgttggg cggctgatat tcatatcacc
    28081 ccggatggtc gtcatttata cgcctgcgac cgtaccgcca gcctgattac cgttttcagc
    28141 gtttcggaag atggcagcgt gttgagtaaa gaaggcttcc agccaacgga aacccagccg
    28201 cgcggcttca atgttgatca cagcggcaag tatctgattg ccgccgggca aaaatctcac
    28261 cacatctcgg tatacgaaat tgttggcgag caggggctac tgcatgaaaa aggccgctat
    28321 gcggtcgggc agggaccaat gtgggtggtg gttaacgcac actaaccgct gatttacccg
    28381 gcgcagtctc ttctgcgccg gtgtattaac ctatctcctg taacgcatgt ctctggcgtt
    28441 cgacgatatt ggtccacaaa ttgtctttat cgtcagtcca caaattaatc agcaaggcaa
    28501 aaaagcgttc agctgccgga gaaagcacgg catctttgcg gcgaataatc cccaatgtcc
    28561 gacgtataac cggttccacc agcgggatac caataatcga agaatagggt gcatggggca
    28621 ttgccagacc aggaagcgcc gaaatcccca gtcccgcctc caccagtcct aatgacgtcg
    28681 aaagatgacg cacttcgtaa aaccagtcca gcttccaggg cttgtcggcc agctgttgtt
    28741 ctatcaacag tcggttgccg ctggaggagc gtacgccaat cattttgtaa cccactaact
    28801 cctgccattc aacaagctgc tttttggcca atggatggtc acgccgacag gccagcacga
    28861 acggctcgtt gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta
    28921 tgccaaagtc tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca
    28981 gaatccgcac tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa
    29041 aataaaatac tgcggtcgga atacacgcta atgtcaccat accacgatga taagcattca
    29101 tatcacgaat attaaaaagc gtttcatcga atttctttat taattcccgc gcctccggga
    29161 gcaacctttt tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgga
    29221 tatgtaaatc ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga
    29281 gtaatttagc ggcattgtta aatgaactgg actctgccag tatgacaaat gccttcatac
    29341 ttgataattc atgcttcatt attactccgg aaaatggaag cgtcgatttt gggtggctgg
    29401 ccgttaaaaa ttttaactgc atttagccaa cttaaattaa tgaaaaaatg ttattaatcg
    29461 ttgagctaaa gtcattagag atgctttgcc cttaatgtaa ccatatcgca ataagttatg
    29521 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
    29581 ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc agcgcgataa
    29641 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
    29701 taatccgctg accagtaaag tcgccattat tagccgttcc agcgatccgc gtgctgatgt
    29761 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
    29821 cggcaatatg ctgtctggcg ttggggcatt cgccattgaa aatggtttga ttgcagcgac
    29881 ttcgccagtt acccgcgtac gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga
    29941 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
    30001 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gccggaacca aaaccggaaa
    30061 agtcttcccg acggataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
    30121 tatggcgatg ccagttgtca ttattcctgc tgaatatctg ggaaaaacag gctatgaatt
    30181 accggcggag ctggatgccg acaaagcatt attagcccgc attgaatcta tccgtctaca
    30241 agcgggtaaa gcaatgggct taggcgatgt cagtaatatg gttatcccta aacctgtgct
    30301 tatttctcca gcgcagaaag gcggagcaat taatgtgcgt tattttatgc cgcattcttg
    30361 ccatcgcgcg ctggcgataa ccggtgctat tgccatttcc agtagttgtg cattggaagg
    30421 caccgtcacc cgacaaatcg tcccttctgt aggatacggc aatatcaata ttgaacaccc
    30481 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
    30541 atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttttcttc cctgaaaaaa
    30601 ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata cagaaagaac
    30661 aggattttaa atgaataaaa aatcgttatg gaagctaatt ctgatattag cgatcccatg
    30721 tattattggc tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
    30781 tatttacctg gcggccattg tggggctggt tatcaagcct ttcccggaac ctgtcgtact
    30841 gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg gtgcgtttaa
    30901 gaccaccgcc gtattaagcg gttactcttc aggtaccacc tggctggtgt tctcggcgtt
    30961 taccttaagc gccgcatttg tgaccaccgg cttaggtaaa cgtattgcct atctgctgat
    31021 tggtaaaatc ggtaacacca cgctgggtct gggttacgtt acggtattcc tcgatctggt
    31081 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt taccgatcat
    31141 caacagcgtg gcggtggctt tggggtcaga accggaaaaa agtccgcgtc gtgttggaca
    31201 ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt tctttaccgc
    31261 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
    31321 ctggggtgga tgggcgctgg ccgccggatt gccgggcatc attatgctgc tggtcacccc
    31381 gctggtgatt tacaccatgt atccgccaga aattaagaag gtggataata aaaccatcgc
    31441 caaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
    31501 ctttgtgctg gcgctgctgg gctggatttt cagtaagtcc ctgggggttg atgaatccac
    31561 cgtggcaatc gttgttatgg cgaccatgct gctgctgggt atcgttacct gggaagacgt
    31621 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
    31681 ctccttatta tcgaaagtta aattcttcga atggttagct gaagtcttta aaaataacct
    31741 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
    31801 tcgttatttc ttcgcttccg gtagtgccta tatcgttgct atgttaccgg tatttgccat
    31861 gctggcgaac gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctccaa
    31921 ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca tctttggcgt
    31981 gggttataac gatattaaat cctggtggtt ggtcggtgcg gtactgacga tattaacctt
    32041 cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct
    32101 gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt
    32161 cacttactta tgtaagctcc tggggattca ctcgcttgtc gccttcctgc aactcgaatt
    32221 atttagagta tatccattta ttatctttct gcgcacttct cggtgcgcag atatctggag
    32281 catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagtaataa cgaaataatt
    32341 gccgaagaac atttcaccgg cgaaattaaa aaagaagaag caaaaaaagg cactattgcc
    32401 tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa aattaagttt
    32461 gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc taaagcgtcc
    32521 ggtatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc actttgcgcc
    32581 gtcggtggca ccattaacgg tgatgaccat gtttttggtt tatcggcggc tcagcgttat
    32641 ggcggtattt ttgtgcctcc gcatattgcg gtcatccatc aatatatgcg tgagatgatg
    32701 gcaggcggcg gcaaaatgat cctcgggtca gacagccaca cccgttacgg tgcattaggg
    32761 acaatggcag tcggtgaggg cggcggcgag ttggtaaaac aattgcttaa tgacacctgg
    32821 gatatcgact atccgggagt tgttgcggtg catctgaccg gaaaaccagc gccgtatgtg
    32881 gggccacagg atgtggcgct ggctatcatc ggtgccgtgt tcaaaaacgg ctacgtcaaa
    32941 aacaaagtga tggaattcgt aggtcccggt gttgctgcgc tctctaccga tttccgtaac
    33001 agcgttgacg ttatgaccac ggaaacgacc tgtttaagtt ctgtctggca aaccgatgaa
    33061 gaagtccata actggctggc gctgcacggt cgcggccagg attactgcca gcttaaccct
    33121 caaccgatgg cgtactacga tggctgcatc agcgttgatt taagcgccat caaaccaatg
    33181 attgcgctgc cgtttcaccc gagcaacgtg tatgaaatcg acacgctgaa ccagaacctg
    33241 accgatattc tgcgtgagat tgaaattgag tccgagcgcg tggcgcacgg taaagccaaa
    33301 ctctcgctgc tggataaagt cgaaaacggt cgcctgaaag tgcagcaggg gattatcgcg
    33361 ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcact gcgcggtcaa
    33421 tcctgtggca atgacacctt ctcgttagcg gtttacccgt catcacagcc ggtgtttatg
    33481 gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat catcagaacc
    33541 gcgttctgcg gcccatgctt tggcgcgggc gatacgccaa tcaacaacgg tttgagtatt
    33601 cgccacacca cgcgtaactt cccgaaccgc gaaggctcta agccagccaa tgggcagatg
    33661 tcagcggtgg cgttgatgga cgctcgttct atcgctgcga ctgcggcaaa cggtggctat
    33721 ttaacctctg ccagcgaact tgattgctgg gacaacgtac cggagtacgc cttcgatgta
    33781 acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag gggcgaccca gcaaccgttg
    33841 atttacggac cgaacataaa agactggcca gaattggggg cgctaactga caatatcgtc
    33901 ctcaaagtgt gctcgaagat cctcgacgaa gtgaccacca ccgacgaact gatcccttcc
    33961 ggtgaaacct cttcttatcg ttcaaatccg attggtctgg cggagtttac cctgtcacgc
    34021 cgcgatccag gttatgttgg cagaagtaaa gcgactgctg agctggaaaa tcagcgtctg
    34081 gcggggaatg tcagcgagct gacagaggtg tttgcgcgca ttaagcagat tgctggtcag
    34141 gagcatattg atccgctgca aactgaaatt ggcagcatgg tctatgcggt gaaaccaggc
    34201 gatggttctg cgcgtgagca ggcggcgagc tgccagcgtg tgattggcgg tctggcgaat
    34261 attgccgagg agtacgcgac taaacgctac cgttctaacg tcatcaactg ggggatgtta
    34321 ccgctgcaga tggcggaagc accaacgttt gaagtggggg attacattta catccctggc
    34381 attaaagcag cgctggataa tccgggtacg acgtttaaag gttatgtgat ccatgaagat
    34441 gcgccggtaa cggaaattac gctttacatg gaaagcctga cggccgaaga gcgcgagatt
    34501 atcaaggcgg gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa agcgccatgt
    34561 gaatgtaggt cgcattcggc acttattgtc ggatgcgatg ctggcgcatc ttatccgacc
    34621 tacaaatcgc atcgaaccgt aggccggata aggcgtttac gccgcatccg gcaaatagtt
    34681 aatagctctt acttcttcgc ctctgcaacc actttgctac ccacgccgcg gttattgtat
    34741 tcccacatgc ggttgtagtt agtgtcattc agattgcgtt gtatttcgtc gttatcatca
    34801 acgctgccgg tgttacccgc aaacggacga ttagagatca ccgcatcagc ccagggtttg
    34861 gctgtgttaa aaccttcgtt gatggcgcta tcacggatca ccacctgacc gttggtattg
    34921 gcatcaacat ccagcgagcg acccagttgc gccacgccat caccggaagc attgaaacgg
    34981 ctgtttacgg cgaggaaacc gtaatagatg ttagacagcg tagccggtgc aaacacatac
    35041 gcttcttgct gagtacgtga gttcaccacg cggaattcgg tgttatcgaa caccactgcg
    35101 ccgcgaccag aaacgatatc cacatcccct tcaatgtaac tgttggtcac cagagtacgc
    35161 ggctgacggt tggtttccag acggttttgc acaccactgt tggtgacaaa gaaggtattc
    35221 tgacgaccga gaatgttgac gttattgatc tgcactttgt caccatcagt acgcagtgcc
    35281 accgccggat ggttacccgc atctacgcta tcgcccagcg tgttttcgat ggtcagattt
    35341 tgcagttgca ggccattgtt ttgtgaccag aagaccgcag agcagagaac accgatactg
    35401 tcgctgcgtt tgctctggca gctatcgtac atataccacg ctggtttacc tggcatatat
    35461 ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc tcatgccccc atcaagggaa
    35521 agcccaatct tcacatcaat cggtttttca cctgtaccgt acagagtaat tccacccgga
    35581 gcggcaggga catacaccgt tccctgatac tcaccaggca tcacggcaat atactggcgc
    35641 ttgttggtgc gcttgataat tgccgcatct accgccgcct gaatcgtggt atgcgttaca
    35701 ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg caggcagggt aatcggggaa
    35761 ggattccacg ctgccgcacc tggtgtcagg gatgcaaaat agtgttgagc atcgaaattc
    35821 tgcgcttctt ttgccgacag aatcgggcga gaagaggtac caggcgcggt ttgatcagaa
    35881 ggacgttgat cgggcggtgt tgagctacag gcggtcagcg tcacgccaaa agccaatgcc
    35941 agcgccagac gggaaactga aaatgtgttc acaggttgct ccgggctatg aaatagaaaa
    36001 atgaatccgt tgaagcctgc ttttttatac taacttgagc gaaacgggaa ggtaaaaaga
    36061 caaaaagttg tttttaatac ctttaagtga taccagatgg catagcgcca tctggcagag
    36121 tgattaacta aacatcgcag taatcgaggc gcttgccaga gaatggaaat ggacgttaaa
    36181 cccgaccatc gcgccgctgg caccttcatc gacatcaata cgttctacat ccagcgcgtg
    36241 aacggtaaaa atgtagcgat gggtttcgcc tttcggcggc gctgcaccat cgtacccggt
    36301 tttaccaaag tcggtacgcg tctgcaaaac gccgtctggc attgctacca gaccagagcc
    36361 aaacccttgc ggtaatacgc gggtatcagc gggtaaatta acaactaccc agtgccacca
    36421 gccggagccg gttggcgcat ccgggtcgta gcaggtgaca acaaaacttt tcgttcccgc
    36481 aggaacatca tcccacgcca gatgcggtga aacattatcg ccatcgtaac ccatgccgtt
    36541 aaagacatga cgatgtggca acttatcgcc atcgcgcaga tcattactga tgagtttcat
    36601 gaaccctcct ttctagtttg cagaaagtgt agccagaaac cctcacgctg acttcccgtt
    36661 attggcaaaa aaatgtttca tcctgcaccg cacgattaac cgctgcggtc agacgctgca
    36721 actgttgcgg gagaataata tagggcggca tcaggtaaat cagtttgcca aaaggccgga
    36781 tccagacacc ctgttcgaca aagaattttt gcagcgctgc catattcacc ggacgagtgg
    36841 tttcgaccac gccaatggcc cctagcacgc gcacatcggc aaccattttg gcatcacggg
    36901 ctggtgccag ttgctcgcgc agttgtactt caatagccac cacctgctgc tgccagtcgc
    36961 cagattcgag aatcgccagg ctggcgtttg ctgccgcgca ggctagcgga ttgcccataa
    37021 aagttggccc atgcataaag caaccggctt cgccgttact gatggtttcc gcaacctcgc
    37081 gcgtggtgag tgtggcggaa agggtcattg taccgccggt taaggcttta ccgaggcaca
    37141 aaatgtccgg cgcgatatct gcatgttcac aggcaaacag tttgccagta cgaccaaatc
    37201 cggtggcgat ctcgtcggca atcagcaaga taccttcgcg atcgcacatt ttgcggattc
    37261 gttttaacca ttccggatgg tagatgcgca tcccgcctgc gccctggaca atcggttcaa
    37321 tgatcaccgc cgcgatttca tgacgatgcg ccgccatcag gcgggcaaag cccaccatat
    37381 cgcgctcatc ccattcacca tccatgcggc tttgcggggc gggagcaaac aagttttctg
    37441 gcagatagcc tttccacaga ctgtgcattg agttatccgg atcgcacacc gacatcgcgc
    37501 caaaggtatc gccatgataa ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt
    37561 tggcttgcca gtactgcaac gccattttca tcgccacttc caccgctacg gaaccggagt
    37621 ccgcgagaaa aacgcattcc agcggttgcg gcgtcattgc caccagtttg cggcacagct
    37681 caatggctgg cgcatgggtg ataccgccaa acatcacatg cgacatggca tcaatttgcg
    37741 acttcatcgc cgcattaagc tgcgggtgat tgtaaccgtg gatcgccgcc caccaggacg
    37801 acataccgtc aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgctcg
    37861 ccaccggata aaccggtagc ggggaggtca tggatgtgta tgggtgccag atatggcgtt
    37921 ggtcaaaggc aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta
    37981 ggtttacgag tctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct
    38041 caccgcccac gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat
    38101 ctgctgtttg aagcgcagca ggtgcatcgt cagcatttcg atccacgtca ggtgcaggtc
    38161 agcactttgc tgtcaattaa gaccggagct tgtccggaag attgcaaata ctgcccgcaa
    38221 agctcgcgct acaaaaccgg gctggaagcc gagcggttga tggaagttga acaggtgctg
    38281 gagtcggcgc gcaaagcgaa agcggcagga tcgacgcgct tctgcatggg cgcggcgtgg
    38341 aagaatcccc acgaacgcga tatgccctac ctggaacaaa tggtgcaggg agtaaaagcg
    38401 atggggctgg aggcgtgtat gacgctgggt acgttgagtg aatctcaggc gcagcgcctt
    38461 gcgaatgccg ggctggatta ctacaaccac aacctcgaca cctcgccgga gttttacggc
    38521 aatatcatca ccacacgcac ttatcaggaa cgcctcgata cgctggaaaa agtgcgcgat
    38581 gccgggatca aagtctgctc aggtggcatt gtgggtttag gcgaaacggt aaaagatcgc
    38641 gccggattat tactgcaact ggcaaacctg ccgacgccgc cggaaagcgt gccaatcaac
    38701 atgctcgtga aggtgaaagg tactccactg gcagataacg atgatgtcga tgcctttgat
    38761 tttattcgca ccattgccgt agcgcggatc atgatgccga cctcttacgt gcgcctttct
    38821 gccggacgcg agcagatgaa cgaacagact caggcgatgt gctttatggc cggggctaac
    38881 tcgattttct acggttgcaa actgctaacc acgccgaatc cggaagaaga taaagacctg
    38941 caactgttcc gcaaactggg gctaaatcca cagcaaactg ccgtgctggc aggggataac
    39001 gaacaacagc agcgtctgga gcaggcactg atgaccccgg acactgacga atattacaac
    39061 gcggcagcac tatgagctgg caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg
    39121 ccgatgccct gcgtcgccgt tatccggtgg cgcaaggggc cggacgctgg ctggtggcgg
    39181 atgatcgcca gtatctgaac ttttcctgca atgattacct cggcttaagc catcatccgg
    39241 acatcattcg tgcatggaag cagggagcgg agcaatttgg cgtcggtagc ggcggttccg
    39301 gtcacgtcag cggttatagc gtggcgcatc aggcgctgga agaagaactg gccgagtggc
    39361 tgggctattc gcgggcactg ctgtttatct ctggttttgc cgctaaccag gcagttatta
    39421 ccgcgatgac ggcgaaagag gaccgtattg ttgccgatcg gctgagtcac gcctcattgc
    39481 tggaagctgc cagtttaagc ccggcgcagc ttcgccgttt tacccataac gatgtcgctc
    39541 atctggcgcg actgcttgct tccccctgtc cggggcagca actggtggtg acagaaggcg
    39601 tgttcagcat ggacggcgat agtgcgccac tggcggaaat ccagcaggta acgcaacagc
    39661 acaatggctg gttgatggtc gatgatgccc acggcacggg cgttatcggg gagcaggggc
    39721 gcggcagctg ctggttgcaa aaggtaaatc cagaattgct ggtggtgact tttggcaaag
    39781 gatttggcgt cagcggggca gcggtgcttt gctccgatgc ggtggcggat tatctactgc
    39841 aatttgcccg ccatcttatt tacagcacca gtatgccgcc cgctcaggcg caggcattac
    39901 gtgcgtcgct ggcggtcatt cgcagtgatg agggtgatgc acggcgcgaa aaactggcgg
    39961 cactcattac gcgttttcgt gccggagtac aggatttgcc gtttacgctt gctgattcat
    40021 gcagcgccat ccagccattg atcgtcggtg ataacagccg tgcgttacaa ctggcagaaa
    40081 aactgcgcca gcaaggctgc tgggtcacgg cgattcgccc accaaccgta cctgctggta
    40141 ctgcgcgact gcgcttaaca ctaaccgccg cgcatgaaat gcaggatatc gaccgtctgc
    40201 tggaggtgct gcatggcaac ggttaataaa caagccattg cagcggcatt tggtcgggca
    40261 gccgcgcact atgagcaaca tgcggatcta cagcgccaga gtgctgacgt cttactggca
    40321 atgcttccac agcgtaaata cacccacgta ctggacgcgg gttgtggacc tggctggatg
    40381 agccgccact ggcgggaacg tcacgcgcag gtgacggcgt tagatctctc gccgccaatg
    40441 cttgttcagg cacgccagaa ggatgccgca gaccattatc tggcgggaga tatcgaatcc
    40501 ctgccgttag cgactgcaac cttcgatctt gcatggagca atctcgcggt gcagtggtgc
    40561 ggtaatttat ccacggcact ccgcgagcta tacagggtgg tgcgccccgg aggcgtggtc
    40621 gcgtttacca cgctggtgca gggatcgctc cctgaattac atcaggcgtg gcaggtggtg
    40681 gacgagcgcc cgcatgctaa tcgcttttta ccgccagata aaatcgaaca gtcgctgaac
    40741 ggcctgcatt atcaacatca tattcagccc atcacgctgt ggtttgatga tgcgctcagt
    40801 gccatgcgtt cgctgaaagg catcggtgcc acgcatcttc atgaagggcg cgacccgcga
    40861 atattaacgc gttcgcagtt gcagcgatta caactggcct ggccgcaaca acaggggcga
    40921 tatcctctga cgtatcatct ttttttggga gtaattgctc gtgagtaaac gttattttgt
    40981 caccggaaca gataccgaag tggggaaaac tgtcgccagt tgtgcacttt tacaagccgc
    41041 gaaggcagca ggttatcgga cggcaggtta taaaccggtc gcctctggca gcgaaaagac
    41101 cccggaaggc ttacgtaata gtgacgcgct ggcgttacag cacaacagca gcctgcagct
    41161 ggattacgca acggtaaatc cttacacctt cgcagaaccc acttcgccgc acatcatcag
    41221 cgcacaagag ggcaggccga tagaatcatt ggtaatgagc gccggattac gcgggcttga
    41281 acaacaggct gactgggtgt tagtggaagg tgctggcggc tggtttacgc cgctttctga
    41341 cactttcact tttgcggatt gggtaacaca ggaacaactg ccggtgatac tggtagttgg
    41401 tgtgaaactc ggctgtatta atcacgcgat gttgacggca caggcaatac aacacgccgg
    41461 gctgactctg gcgggttggg tggcgaatga tgtcacgcct ccgggaaaac gtcacgctga
    41521 atatatgacc acgctcaccc gcatgattcc cgcgccgctg ctgggagaga tcccctggct
    41581 tgcagaaaat ccagaaaatg cggcaaccgg aaagtacata aaccttgcct tgttgtagcc
    41641 atcctgtatt tggttaaatt gcgagcgaga tcgcgtcttc gattgactgc aatttaacca
    41701 attaaattct aaaataatca cgaaaaaaat tttacttccg catcatgcga tgtatgtggg
    41761 aattgcccag gcggcggggg ataggggctg cagacagtta tccactattc ctgtggataa
    41821 ccatgtgtat tagagttaga aaacacgagg caagcgagag aatacgcggc ttgcacgcga
    41881 attggcgtta aagacggctc aaagaaatat cttttatttt ttaactggtt agataattgc
    41941 aatggcagtc actgaagagg catctcttgc cataaaactg tcatcactca tcttgacaaa
    42001 tgttaaaaaa gccgttgctt tggggataac ccggtaaggc cggagtttta tctcgccaca
    42061 gagtaaattt tgctcatgtt tgacagcgga gtttacgctg taacagaaat attatggtga
    42121 gaaactgttt ttttatccag tataatttgt tggcataatt aagtacgacg agtaaaatta
    42181 catacctgcc cgcccaactc cttcaggtag cgactcatga gtaaaccgtt caaactgaat
    42241 tccgctttta aaccttctgg cgatcagcca gaggcgattc gacgtctcga agaggggctg
    42301 gaagatggcc tggcgcacca gacgttactt ggcgtgactg gctccgggaa aaccttcacc
    42361 attgccaatg tcattgctga tcttcagcgc cctaccatgg tacttgcgcc caacaaaacg
    42421 ctggcggccc agttgtatgg cgaaatgaaa gagttcttcc cggaaaacgc ggtggaatat
    42481 ttcgtctcct actacgacta ctatcagccg gaagcctatg taccgagttc cgacaccttc
    42541 attgagaaag atgcctcggt taacgaacat attgaacaga tgcgtttgtc cgccaccaaa
    42601 gcgatgctgg agcggcgtga tgtggttgtg gtggcgtctg tttccgcgat ttatggtctg
    42661 ggcgatcctg atttatatct caagatgatg ctccatctca cggtcggcat gattatcgat
    42721 cagcgcgcga tcctgcgccg actggcggag ctgcaatacg ctcgtaatga tcaggcattc
    42781 cagcgtggta ctttccgcgt tcgtggtgag gtgattgata tcttcccagc agaatcggat
    42841 gacattgcac ttcgcgtgga gctgtttgac gaggaagtgg aacgattgtc gttatttgac
    42901 ccgctcaccg ggcagattgt ttccactatt ccacgtttta ccatctaccc gaaaacgcac
    42961 tacgtcacgc cgcgcgagcg catcgtacag gcgatggaag agatcaaaga agaactggcc
    43021 gccagacgca aagtgctgtt ggagaataac aaactgctgg aagagcagcg gctgacccag
    43081 cgtacccagt ttgatctgga gatgatgaac gagctgggct actgttccgg gattgagaac
    43141 tactcgcgct tcctctccgg tcgtggaccg ggtgagccac cgccgacgct gtttgattac
    43201 ctgcctgccg atgggctgct ggtggtcgat gaatcacacg tcaccattcc gcaaattggc
    43261 ggcatgtatc gcggtgaccg ggcgcgtaaa gagacgctgg tggagtacgg cttccgcctg
    43321 ccatcagcgc tggataaccg tccgctgaaa tttgaagagt tcgaagcatt agcgccgcaa
    43381 accatctatg tttcggcgac gccgggtaat tacgagctgg aaaaatcggg cggcgatgtg
    43441 gtggatcagg tggtgcgtcc aaccggattg ctggatccga ttatcgaagt gcggccagtg
    43501 gcgacacagg tcgatgatct tctatcggag attcgtcagc gagcggcaat taacgaacgc
    43561 gtactggtta caactctgac caagcgaatg gcggaagatc tcacggaata tctcgaagaa
    43621 cacggcgagc gcgtgcgtta tcttcactcc gatatcgaca ccgtcgagcg tatggagatt
    43681 atccgcgact tgcgtctggg cgagttcgac gtgctggtgg ggatcaactt actgcgcgaa
    43741 ggtctggata tgccggaggt ttcgctggtg gcgatcctcg acgccgacaa agaaggcttc
    43801 ctgcgttctg aacgttcgtt aatccagact attggtcgtg cggcgcgtaa cgttaacggt
    43861 aaagcgattc tctacggcga taagatcacc ccgtcaatgg cgaaagcgat tggcgaaacc
    43921 gaacgtcgtc gcgagaaaca gcagaagtac aacgaggaac acggtattac gccgcagggc
    43981 ttgaacaaga aagtggtcga tatcctggcg ctggggcaga acattgctaa aaccaaagcg
    44041 aagggcagag gaaaatcgcg accgattgtt gagccggata atgtgccgat ggatatgtcg
    44101 cctaaagcgt tgcagcagaa gatccatgaa ctggaagggt tgatgatgca acacgcgcag
    44161 aatctggagt tcgaagaagc ggcgcaaatt cgtgaccagt tgcatcagct gcgtgaattg
    44221 ttcattgccg cgtcgtgaca agatgaaaaa gccggaatgt tcttccggtt cagattaacg
    44281 atcaagtcaa aaaagtgctc gaacggtccc ctcgtccctc cggggagagg gtgaggggaa
    44341 caggtcgcat tgcaccagaa gcctggtcag caatctcggc cacattaacc cagtgcttgt
    44401 aacgcctttt ccagtgcgtt atgtaacaac tggcggtcat gacgatacgg aatatcgctg
    44461 gcctccagta cctcctgaat cacaatccgc tctttcaccg ccgaggcatc cacttttggc
    44521 ccgacgatga ccgcatcaat gaccttttta ccgacatact gctccataat tgccagcttg
    44581 ctttccagct tcaaattagc cgcaggtaaa ctcaactcac gccccagatt gccgatatag
    44641 accatcggcg ctggcgtgcg gcgtaatgcc tgtgcgattt ccttcagcag cagaattggc
    44701 atcaggcttg tataaaaact gccaggccca ataatgatga gatccgcttc attgatagcg
    44761 tgaacagcct cacgcgttgc gggtacattc ggcgttaaca gtaactcctg aatcggcgta
    44821 gttaactggt cgatattgac ctcgccgtaa acttcatgcc cctgatcgtc aatcgccatc
    44881 agatccaccg gatgctccga catcggaatc aaatgtgcgt ccactttcag cagattgcga
    44941 atcagattga tcgcttcaag aggtcgcacg ctaaggtgat ccagtgcctt cagcatcagg
    45001 tttccaaggt tgtgtccgga aagttcgcca ttaccgccga aacgatattc aaacattgcg
    45061 gaagcgacgc tgggttcagt tatcagctgg ttgaggcagt tacgcatatc gccccaggca
    45121 ataccgcctt ctgagcggcg aatacgcccc gtcgatccgc cattatccgt ggtggtgacg
    45181 atacccgtta aacgagaacc caaagacgaa agtgatgaga gaacgcgtcc cagtccatgc
    45241 cctccgccga gagcaacgac acgatcaaga tcagccagcg tacgattgcg catatagttt
    45301 cctggagtca gattatccgc gctacagtag cgcaaattgc ggggaaacag caattaacct
    45361 gccaatattg aggatacaaa ctattttctt ctacctctaa aggatgaagc acgctatgcc
    45421 tctctgatga tgtatatcaa agtaaaaccg ccattttccc ttattctgta gcgaaatagc
    45481 acgatcatga cgctatatac atgattacat agcgaaagtg tggatggtaa aaatctcatt
    45541 tacacgctag tatcggctta accactaaac actctagcct ctgcacctgg gtcaactgat
    45601 acggtgcttt ggccgtgaca atgctcgtaa agattgccac cagggcgaag aaagaaatga
    45661 cttcgcctcc cgtatctgga aaggtgtaca tggcttcaca actgactgat gcatttgcgc
    45721 gtaagtacta ctacttgcgc ctgtcgatta ctgatgtgtg taactttcgt tgcacctact
    45781 gcctgccgga tggctacaaa ccgagcggcg tcaccaataa aggctttctt accgtcgatg
    45841 aaattcgccg ggttacgcgc gcctttgcca gtctgggcac cgaaaaagtg cgcctgacag
    45901 gcggtgagcc gtctttacgc cgcgacttta ccgatatcat cgccgctgtg cgggaaaacg
    45961 acgctatccg ccagattgcg gtcaccacca atggttaccg tctggaacgc gatgtggcga
    46021 actggcgcga tgcgggactt actggcatca acgtcagcgt tgatagcctt gacgctcgcc
    46081 agtttcatgc cattaccggg caggataaat tcaaccaggt catggcaggg atcgatgctg
    46141 catttgaggc cggttttgag aaggtcaaag tcaataccgt gctgatgcgt gatgttaatc
    46201 atcatcagct cgacaccttt ctgaactgga tccagcatcg ccctatccag ctgcgtttca
    46261 tcgaattgat ggaaacgggc gagggcagcg agctcttccg taagcatcac atctctggtc
    46321 aggttctgcg tgacgagcta ctgcgtcgcg gctggatcca ccaattacgt caacgcagtg
    46381 acggtcccgc gcaagtcttt tgtcatccgg attacgccgg agagattggc cttatcatgc
    46441 cgtatgaaaa agacttctgc gccacttgca accgcctgcg cgtttcctcc attggtaaac
    46501 tccatctctg cctgtttggt gaaggcggcg ttaacctgcg cgatctgctg gaagacgata
    46561 cccagcaaca ggcgctggaa gcgcgtattt cagcggcgct gcgggagaag aaacagaccc
    46621 atttcctgca tcaaaacaac accggtatta cgcaaaactt atcgtacatt ggcggctaaa
    46681 acgtcaaaag gagagatcag atgagtcagg taagcactga atttatcccg acccgtattg
    46741 ctattcttac ggtttctaat cgtcgcggtg aagaagacga tacctccggt cactatctgc
    46801 gcgattcggc gcaggaagcg ggccatcacg ttgtcgataa agccattgtg aaagaaaacc
    46861 gctacgctat tcgcgctcag gtatctgcgt ggatcgccag cgacgatgta caagtggtat
    46921 tgattacggg ggggactggc ctgacggaag gagatcaggc tcccgaagcg ctgctgccgt
    46981 tgttcgaccg tgaagttgaa ggttttggtg aagtgttccg gatgttgtcg ttcgaagaga
    47041 ttggcacttc cacgttgcaa tctcgtgcgg tagcgggcgt cgctaacaaa acgctgattt
    47101 tcgccatgcc gggttcgacc aaagcgtgcc gcaccgcgtg ggaaaatatc atcgcgccgc
    47161 agctcgatgc ccgtacacgt ccatgcaatt tccatccaca tttgaagaaa taagtatgtc
    47221 gcaactgacc catatcaacg ccgctggcga agcgcacatg gtggatgtct ccgccaaagc
    47281 ggaaaccgtg cgtgaagcac gggcggaagc ctttgtcacc atgcgcagcg agacgctggc
    47341 gatgattatt gatggtcgcc accataaagg cgacgtattt gccactgcgc gtattgccgg
    47401 tattcaggcg gcaaaacgca cctgggatct gatcccgctg tgtcatccgc tgatgctcag
    47461 caaagttgaa gtcaatttac aggctgagcc ggagcacaat cgggtgcgta tagaaacctt
    47521 atgccgcctg accgggaaaa ccggtgtcga aatggaagcg ttaaccgcgg cctccgtggc
    47581 ggcgctgacc atttatgaca tgtgcaaagc ggtgcaaaaa gatatggtaa ttggtccggt
    47641 acgtttgctg gcgaagagtg gcggcaagtc gggtgacttt aaggtggaag cggatgatta
    47701 aagttctttt tttcgcccag gtgcgcgagt tggtgggaac agatgcaacc gaagtggctg
    47761 cggatttccc aaccgttgaa gcgttacgcc aacatctggc tgcgcagagc gatcgctggg
    47821 cgctggcgct ggaagatggc aaattgctgg ctgccgtcaa ccagacgctg gtgagttttg
    47881 accatccgct gactgacggc gacgaagtgg ctttcttccc gccggtaacc ggaggttaag
    47941 atggcagaaa ccaaaattgt tgtcggcccg cagccattca gcgtaggaga agagtacccg
    48001 tggctggctg agcgtgatga agacggtgcg gtagtcacct ttaccggtaa ggtgcgcaac
    48061 cataaccttg gcgacagcgt caaagcatta accctcgaac actatccggg gatgactgaa
    48121 aaagcactgg cagaaattgt cgatgaagcg cgtaaccgct ggccgctggg gcgcatcacg
    48181 gtgattcacc gcatcgggga attatggccg ggagatgaaa tcgtttttgt cggtgtcacc
    48241 agtgcgcatc gcagcagtgc gtttgaagcc gggcagttta ttatggatta tctcaaaacc
    48301 cgcgcaccgt tctggaagcg tgaagccacg ccggaaggcg accgctgggt tgaagctcgg
    48361 gagagcgatc agcaggcggc aaaacgctgg tagttttttg ttagccggat aaggcaccgc
    48421 tgcgtccggc aatttcaccg ctcagtacat gcgtttacgc tgcgtcgatg cacagcctca
    48481 tcattttgca gtatccttaa gatattcctt atatcttcag gagatcgtca tggacagatt
    48541 cccacgttct gattcaattg tacaaccccg ggctggcttg caaacttata tggcgcaagt
    48601 ctatggctgg atgaccgttg gcttgttgct gaccgcattt gttgcctggt atgcggctaa
    48661 ttccgcggcc gtgatggagc tgttgttcac taaccgtgtc tttttaatcg gtctgatcat
    48721 cgcgcaatta gcattggtta ttgtgttatc agcgatgatt caaaagctga gcgcaggtgt
    48781 aacgacgatg ctctttatgc tttattcggc gctgacgggt cttacgcttt ccagtatttt
    48841 cattgtctat accgctgctt ctatcgccag tactttcgtc gttactgccg ggatgtttgg
    48901 cgcaatgagc ctgtacggtt acaccacgaa gcgcgatcta agcggctttg gcaatatgct
    48961 gtttatggcg ttaatcggca ttgtgctggc atcgctggtc aacttctggc tgaaaagcga
    49021 agcattgatg tgggcagtta cctacatcgg cgtgattgtc tttgtcggat tgacggcgta
    49081 tgacactcag aaattgaaaa atatgggtga gcagattgat acccgcgaca cgtcgaacct
    49141 gcgcaaatat tccattcttg gcgcgttaac cttgtatctg gacttcatca acttgttcct
    49201 gatgttgttg cggatcttcg gcaaccgccg ttaattcttt ttcaccatca atatcccggc
    49261 catgtgtcgg gatttttttc ttactaaaaa cgacacttaa caagtaccag gtataaaata
    49321 tgccttatgg ccgagcggaa gtgttttccg tatccataaa tacaaaagta ttttgtatgc
    49381 gtggttttaa atttattgat ggtgaatatt aatattttat ctaaggataa ctaaaagaat
    49441 ggaatcatac tcgcaaaaca gcaataaatt agattttcag cacgaagcca ggatattaaa
    49501 cggcatatgg ctcattaccg ctttgggtat ggtggcaacc gcagggctgg ccctggtgat
    49561 catcatgaat gccgcattaa tgagtgaacg acccgtttgg gtaataagtt gcttaatgat
    49621 tgtgttatgg tcaggcatta tctcgcatgg acgaaataag ctccttgaac tggcggggaa
    49681 atgccatagt gaagagttgt ggagtccggt tcgttgcgct tttacaggtg cattaactct
    49741 ctattactat tttatcggct tctttgggat acttgcagca atagcgataa cgcttgtctg
    49801 gcaaaggcat acgcgttttt ttcattagtc gtaaatgcac atactaaaga acttaactaa
    49861 acttcacatc gccgcttcat ttttcgcccg cagcttcttc gcctggcttt ccagtagcag
    49921 gtaacagatc agcgccagca gcagtgggat aaagtaatac agcacacggt aagcgagtag
    49981 ggcggcgata attgtacctt tggaggtatg ctccccagcc agtagcgcga taaataccgc
    50041 ttccagcaca ccaattcccg ccggaatatg cacgatgacg ccagcaatac tactaaccag
    50101 taacacgccc agtacaaaga aatagttcac gctttgacca agtaacagcc agataatcgc
    50161 ccccattacc atccagttaa cgctggaaat cagcatctga gcgagggcga atttccatga
    50221 aggcagcacc agcttttgcc ctttgatggt catatggcgg tgcttcgcga aagcgcaaaa
    50281 ccacaaataa accgcgataa tcatcagtaa gccaatgccg agaatacgca gcgtagtttg
    50341 atcgacatac cagtgatccg gcagctccac cacgcccgcg gtaaagataa tccctgccag
    50401 caaaatatag cccagccagt tggtggtaat actgagcgag aaaatccgcg taatagtgct
    50461 gcccggtaac cctagccggg agtacaaacg atagcgcata ccaatgccgc cgacccaggt
    50521 actgagcgtc aggttgaagg cgtagcagat aaacgacacc agcatcacct ggcgcttcgc
    50581 cagtttgtga ccgcagtaaa agcgggcgag cagatcatag cagccgtaaa tcagataact
    50641 gacgaccacc agcccgaccg cactgagcaa cgcaacgcga ttgtagtcgc ggatgacctt
    50701 ccagacctct tcccagtcca cctttttggc gtagaccacc agcaacacaa tcaccgcgat
    50761 aaaaaacagc caggtgagga tcttctttgc taagcgccag cgcgggtgtg atttactcat
    50821 cagggtttta cccccgtgtt ttcagtttct acccgatcct gcgtttccat tgtcggctga
    50881 gcgggcgggt caacctgcgc cagacgtggt gtgtgtgccg gaagccagcc gaccagcgcc
    50941 gggaagtggc gtaaaaagtg gaaggccagc acgcttttgg tcagattcca ccaggtgcgt
    51001 ttcggcagca tggtttcatc cacctgctgg cagtctgcgg cgataatgcc gttcagatta
    51061 tcgcgcagcg tctggttaaa atgacgatcg tggatgatga catttgcttc gagattcagt
    51121 gacaaactga gcggatcgag attactggac cctaccgtcg cccagtgatc gtccatcaat
    51181 gccactttgc catgtagcgg acggcggcgg tactcaaaca cctgaacgcc gcctttaacc
    51241 agatagttat acagcaagcg tgcaccgact ctgacaatcg gcatatccgg ttcgccctga
    51301 atgatcagtt tgatccgcac cccgcgccgt gccgctttac gcaaggcgtg taaaaatcga
    51361 tagccgggga agaagtaggc gttggcgata atgacttctc gccgcgcctg agtgagcatt
    51421 ttcagatagt gacgttcaat atcatcgcga tgttcttcgt tatcgcgcca gaccagcaat
    51481 acctgcgctt ctcccggctg gcggttctct tccgctttgt gatgacgtcg ccaccagcgt
    51541 cgtgccgcgc tctgtcctgg caggttttcc agttcaaatt gcagaatatc ttcgacaatc
    51601 ggcccttcaa ggcgcaccgc gtaatcctgt ttagcctccg gaccgtagct ggacatatgc
    51661 tcggcggagt agttcagccc gccaataaag gcaatacgcg cgtcgatcac cacaattttg
    51721 cgatgcatcc ggcgaaacac attggtgcgc ataccaaaaa ggcgagggcg gggatcgtag
    51781 tagcgaaata ccacgccagc tgccgtcaat tcattgacaa actcatcgct gagatccggc
    51841 gaaccgtagc catccagcaa gacttccgct ttcaccccgc gttgcgctgc tgccagtagc
    51901 gccgcatgca gctgtttgcc gacgtcatct tcaaaccaga taaacgtttc aagaatgatg
    51961 cgttcttgcg cctcgccaat cgccttaaac accgcgggat aatattgctc gccgttttcc
    52021 agcaactgga tcttattgcc ttcgcgccag ctacatttca taaatgaatc tccgcactta
    52081 aaggggcatg atcagaaagg tgtcgccatg tccgcagcgg caacgcggtt ggcgcgctgg
    52141 cgctggcatt tttgacatag atcctgtcca gacgtagtag aggaaattgc accggaaacg
    52201 tgcgcgccgg gcgtccgtgg gcgcgggtaa atatctcatc cagcccggcc tgcactttta
    52261 acggatgatt agctttttgc cgccagtcgt tgaaatcacc cgccaccaat accggttcgc
    52321 cgtccggcag ctcattcacc cattcggcga gcatcgcaag ctgcgcctga cggtgcgcct
    52381 cacgcaggcc cagatgtacg cacatcacat gaatcgcttt cccggtcatc ggcggcacaa
    52441 tgcggcagta gagcacgccg cgcttttccg caccatcgac cgaaacatca cgattctcat
    52501 aatgttcaat gggataacgt gaaagaacgg cgttaccgtg atgtccttcc gggtatacgg
    52561 cattgcgacc gtaggcaaaa tcgctccaca tagtgtcggc gagaaactcg tagtgcgagg
    52621 tatcgggcca gttttccaca tgcagcggat gaacttcgtg cgcgcccatc acttcctgca
    52681 ggcaaacaat atcggcgctg acggtacgca cggcgtcgcg aagttccggc aaaatgaagc
    52741 gtcggttaaa cgcggtaaag cctttgtgaa tattgatggt gagcaccttg aacgaaaatt
    52801 gttgtgtttg atcgggcata attttcctgt ctttgcctct tatctcattg aaatagtgta
    52861 gtcggcgtca caaaaaggtg cggtcttacg gaattttccg taaagttcgg tactctgagt
    52921 aagtagagat aaattcttca ggagagaagc catgaagtgg caacaacgtg ttcgtgtcgc
    52981 aacgggtcta agttgctggc agattatgtt gcatttactg gtagtggcgc tgctggtggt
    53041 gggctggatg agtaagactc tggttcacgt cggcgtggga ttatgcgcac tgtattgtgt
    53101 cacggtagtg atgatgctgg tgtttcagcg ccaccccgag caacgctggc gtgaggtggc
    53161 agacgtgctg gaagagctga ccacgacctg gtattttggc gcagcgctga ttgtgctgtg
    53221 gctgttgtcc cgcgtactgg aaaacaactt tttgctggcg attgcagggc tggcaatcct
    53281 tgccggcccg gcggtggtgt ctttgctggc gaaagataag aagttacatc accttacgtc
    53341 taaacatcgc gtacgccgct gaccctgtcg tggccgttat caccagtagc ggccacaaac
    53401 tattccacac aatatccaga ctcgcatcct tcaaataaat ctgcttggta atgtccgtaa
    53461 agtggcgaat agggttaatc cacgtcaggt tttgcagcca tactggcatg ttttccaccg
    53521 gagaaacgta accggaaagg agaatggcgg gcatcataaa aacaaacacg ccgataaacg
    53581 cctgctgttg tgtcgagcag agtgatgaaa tcaacagacc gaatcccacc agcgataaac
    53641 cgtaaatcac catcgtaaag tagaacagcg ccagcgatcc ggcgaagggg atttgatacg
    53701 cccagatacc aatcgccagc acaatggtgg cctggaaggt ggcgacaatt aatgccggta
    53761 cggccttgcc gatgaagatc tgccaggtgg tgagcggcga aaccagtagc tgatcgagcg
    53821 taccttgttc acgttcgcgg gcaacagaaa gtgaagtgac gatcattacg ccgatagtgg
    53881 tgatcatggc gatcagcgac ggcaccacaa accatttgta gtcgagattc gggttatacc
    53941 agttgcgtac caccagctcg ctgttgttag gtttcggttt tccttccagc agctcctgct
    54001 gataattttt gacgatctgt tgcaggtagt tggcggcaat ttgcgcactg ttggagttac
    54061 gcccgtcgag gatcaactgc aacggcgcgg tctggaaggt atccagtttg cgcgagaagt
    54121 cagccgggaa acgcaccagt agtaacgcct tttgtgtgtc gatggttggg cggatctcct
    54181 gtgggctttt cagcagcagc acatgagtaa aggcgctggc gcgggcaaaa cgttgggtca
    54241 gctccaccga atgctcgccg ttatcttcat cgtagatggc gatggtggcg ttagtcactt
    54301 ccagcgtggc ggcgaacggg aacaggatca cctgaatcag cacgggtaaa ataagaatcg
    54361 cacgggtttg cggttcgcgc agcagcgact gcaactcttt gcggattaac gtccataagc
    54421 gatgaaacat gctcttctcc ctaatccagc cgacgtttgg ttttcagcca cgtcaggccg
    54481 ataaacatca ccgccgaagc gatcaaaaac agtacgttca ccaccagcac caccggaata
    54541 ttcccggcga gaaacaggct ttgcagggtg ctgacgaaat aacgtgcggg aataatgtac
    54601 gtcaccgcgc ggatcaccgc gggcatactg tcgatctgaa aaataaagcc ggaaagcata
    54661 atcgacggca gaaaagccgc gttcagggcg acctgagcgg cattgaactg gttgcgggta
    54721 atcgtggaaa taagcagccc catccccagg gtactgagta aaaacaggct ggagataaaa
    54781 aacagaatca gcagcgaccc gcgatacggc acgccgagaa taaacaccga caccagcata
    54841 cacagcaaca tcgccagcat cccgaggaaa tagtagggta tgagcttaca cagcagcagt
    54901 tccgtgcggg taatctccgt agagagcaga gcctccatgg tgccgcgttc ccattctcgc
    54961 gccaccacca gcgaggtgag aatcgcgccg atgaccgtca tgatgatggt caccgcaccg
    55021 gggataatga agtgctggct aatcgccgcc gggttaaacc agtagcgggt ttgtacatca
    55081 ataagcggtt caaaagtctg cccgttgtcc tccgctcgct gcatttgcca gatctgccag
    55141 atcccttcga catacccctg cacaaagtta gcggtattcg gctcactgcc gtcggtgatc
    55201 acctgaatcg gtgcggtggc gttggcgcgc tccatctgtt cggcgaaatc caccggaata
    55261 accaccagac cgcgaatttt ccccgcctgc attttggcga tcagttcctg acggttatcg
    55321 ctgatggtgg cgtcgatgta gggcgaacct gtcatggtgt gggtgaaatc cagcgccgct
    55381 tcgctacgct gttccagcaa tatcccgacc cgcagcttgc tggagtcgag gttaatgccg
    55441 tagccaaaaa taaacagcag cagcagaggg atcactaccg caatcagcca gctactcgga
    55501 tcgcgaacga tctgccgtgt ctctttgacg cacagcgccc gcacgcgacg ccaggacagg
    55561 atcggattac tcattgctat gctccttatc ccagtcgtgg atcaactgaa taaaggcttg
    55621 ctccatagtg ggatcgggtt gctcatcgtt ggccgactgc gctttcaaat cgtccggtgt
    55681 gccgctggcg attaatttcc cgcggtacac caggccgatg cggtcgcaat attccgcttc
    55741 atccataaag tgggtggtga ccatcaccgt gacgcctttc tctaccatac tgttgatatg
    55801 tagccaaaat tcacggcggg tgatgggatc aacgccggac gtcggttcgt cgagaaacag
    55861 aatgtccggt tcatgcatca gcgagcaggc cagtgccagc cgctgtttaa aacctaatgg
    55921 cagttcatcg gtcgcgtggg aggcgatact tttcaggccg aacgcctcgc tcatacggga
    55981 gattttttcg ttctgcgccc gaccgcgtaa gccatacaca ccagagaaaa agcgtaaatt
    56041 ctgttcgacc gtcaggttgc cgtagagcga aaatttttgc gccatatagc cgagatgctg
    56101 gcgcgcttta ccggaactct ctttcagatc catgcccagc accagcgcct ggccagaggt
    56161 cggtaccagc aaaccgcaca tcatcttaaa ggtggtcgat ttacccgcgc cgtttggtcc
    56221 cagcaaacca aaaatctccc cgcgtttaac ggcaaagttg acgtgatcgg tggcggcaaa
    56281 atcaccaaat ttcttggtta gttccttcgc ttcgatcacc gtctcgccgg gtgtgccttc
    56341 taccgtatgt aatattgcgc ccagcggcga ttccgaggtt ccggcaccgc ccagcaaatc
    56401 aataaacgca tcttcaaaac gtggcgtagt ttcgttgatg ttgatttccg gcatcccgtc
    56461 ggcatggcga atatcgtctg gtgtggtctc ttttttgagg atcagtcgta ccgatttccc
    56521 ctgaatcatg ccgtcgctga cctgcggcag tttcaaggcg cgttgcaaca gtttgcggtt
    56581 gccctcgtgc gggctggtca tcagaaagct gcgtccggcc atggtttgtg tcagggccgt
    56641 cggttctccc tggtacaaca attcaccttc gttcatcagt aacacgtcac ggcactgctc
    56701 ggcttcgtcg agatacgagg tactccagag gattaacatc ccttcgcctg ccagctcatg
    56761 caccatctgc cacagttcgc gccgtgagat agggtcaacg ccgacgccgg gttcatcgag
    56821 cagcaacact ttcggttcgc ccaccagggt acaggccagg ccaagttttt gtttcatccc
    56881 accggagagc ttgcccgcca ggcgtccggt aaacgggcca agagatgtaa actccagcag
    56941 gcgagcaaaa gtttgcttcc gtgcctcgcc ggtgacgctg cgcaaatccg cgtacagatt
    57001 gaggttctcc atcaccgtca gatcttcata cagaccgaat ttctgcggca tatagccgag
    57061 cacggcgtgc agcgcgccgt cgtttttgat tggatcaaag ccaatcactg tggcactgcc
    57121 gctgtcgggt ttcagtaatc ccgccagcat ccgcatcagc gtggttttgc ccgcaccgtc
    57181 tggccctacc agtcccgtca cataaccggc atgaatggtg caatcgagcg gcgcgacggc
    57241 gggcttgtcc atgcccggaa agcgtttttc caggccgttc agcgtgataa cggcatcatt
    57301 catgtcctgc ctcgtcaccg aattgtaccg tcactggcat gccctggcgt aacgcatcat
    57361 cggcgtcggt caccacaata cgcaggcgat agacgaggtc ggtacgcaga tccggcgttt
    57421 cgacggtttt cggggtaaat tcagcagtcg gtgaaacgaa accaatctgc ccgtgatacg
    57481 gcttgtccgg gcgaccatcg gtataaagca acactttgcg ccccggctgg gcctggtcaa
    57541 gattacgttc atcaacatag gcgcgtaccc acaccggacg cgtcagcgag acggtaaaca
    57601 ccgtgccacc ttcattgaga accgtgcctg gctccaccgc gcgcgttaac agcgtgccat
    57661 cagacggggc tatcaacgtt gagtcctgta aattcagctc cgcctgcgcc agttgcgcct
    57721 gcgcctgttc gaggctggct tttgcctggg cgatatcctg ttcgcggtta ccggaacggt
    57781 actgacgcaa tttatcctgt gccgatttca gcgttgcctg cgcctggtcg cgcgaggagc
    57841 gggcattttc caggtcattt gccgaaatgg tgcggctttt ccacagtcct tgctggcggt
    57901 tgtagaagtt ctgcgcatag tcataggcag cttgcgcctg tttcaccgct gcggcggcct
    57961 gagcgatttc ttcatcgcga tacccggcaa gcatcaggtc atactgcgcc tgcgccaccg
    58021 aaacgcccgc tttcgcctgc atcagggcaa tctcatacgg cttgtgatcc agttcgccca
    58081 gcacctggcc cgctttgata gcatcacctt cgtccaccgc cagcgattca acgcgccccc
    58141 caacacggaa actaagattc accgtacgaa tatccacgtt gccatacagc gtcaggccgt
    58201 tatcctggcg gctttgatac caccagtagc ctccggcaac cacggcggca agtactacta
    58261 ccgccagtcc gatcacgaca ggttttttca tcactacaaa ctcctttgcg ataatccttg
    58321 caaaatcagg tcgatatgac aggtcaccgt ctggttgatc agttcggtct tttcttcatc
    58381 gaacgcggtc cagccggtac gtaacagaat tgtttctttg ccaagacgaa acgccagaat
    58441 ctcaccaatc aacgcatggg tatgaaggat catgcgggtg tcattggcgt cgcagccggt
    58501 ccaggcggca atcaggcgtg tcaggtggct gtgtagcgga ctaatcacct gttcgtgcac
    58561 caggtggtag gctgccgtgg gagagagctg ctcacgggag ataaacttgc tgaggttgac
    58621 agtgtcatcc tgggtgagca gcttaatcat gttcctgcag gcgcgaagga tcagttcacg
    58681 gatggcagcc cgatctggct gtggttgtgc gaacaagcgt tcggcctctt cggcgtgcgg
    58741 acggaactgc tcgccaataa aatcggcaat ccactgggcg caggcgaggt acaaatcctc
    58801 tttcgaaccg aagtagtagg tgatggcagc gatattctgc ccggcctggg cggctatctc
    58861 gcgggtggtg gcgttcattc catattcacc aaactgcgcc agtgcggcag caatcagctg
    58921 ttttttcgcc tgttcaccct tgattgtcat ggcaggatta ttcatcgcac agtccattct
    58981 taatcaaatg attgattaag attatgactc catatgagag ttgtccagta tggctaagaa
    59041 ttttagcaac gccagtcaca gggataattt atgcgctgcg tcacaaaaac tgctacactc
    59101 cgctccctca tgacattgtg gtttttgtca ttttcctttt cagtatctcc ctgaaaacta
    59161 caccggtaac ggtcggggcg gttcggagta gttatgtctt tcgattcttt gggtttaagc
    59221 cctgatatcc tgcgcgccgt tgccgagcag ggttaccgtg aacccacccc tattcagcag
    59281 caggcgatcc ctgcggtgat ggaaggccgc gacctgatgg ccagtgccca gaccggcacc
    59341 ggcaaaacag cgggctttac gctgccgctg ttgcaacacc tgattactcg ccagccgcac
    59401 gccaaagggc gtcgtccggt acgtgcgctc attcttaccc cgacccgtga actggcggcg
    59461 cagattggcg aaaacgtccg tgattacagc aaatacctga acattcgttc gctggtggtg
    59521 tttggcggtg tcagcattaa cccgcagatg atgaagttgc gcggcggcgt tgatgtgctg
    59581 gtggcaacgc cgggacgtct gctggacctg gaacatcaga atgcggtgaa gctggatcag
    59641 gttgaaatcc tcgtcctcga tgaagctgac cgcatgctcg acatgggctt tatccacgat
    59701 atccgtcgcg tgttaacaaa actacctgcg aagcgccaga acctgttgtt ctccgcgacc
    59761 ttctctgacg atattaaagc tctggcggaa aaactgttgc acaacccgct ggaaatcgaa
    59821 gtggcacgcc gcaataccgc gtctgatcag gtgacccagc atgttcactt tgttgataag
    59881 aaacgcaaac gcgaattgtt gtcgcacatg attgggaaag ggaactggca gcaggtgctg
    59941 gtgtttaccc gtaccaaaca tggcgctaac catctggctg aacagctcaa taaagatggc
    60001 atccgcagtg cggcgatcca cggcaataaa tcgcaaggtg cgcgtacccg tgcgctggct
    60061 gattttaaat cgggcgatat tcgtgtactg gtggcaactg acatcgctgc gcgcggcctg
    60121 gatattgaag agctgccgca cgtggtcaac tatgaactgc caaacgtacc ggaagattat
    60181 gtccaccgta tcgggcgtac cggtcgtgcg gctgctaccg gtgaagcgtt gtcgctggtg
    60241 tgtgttgatg aacacaaact gctgcgtgat atcgaaaaac tgctgaaaaa agagatcccg
    60301 cgcattgcga ttccgggcta tgagccggac ccgtcaatca aagccgaacc aatccagaac
    60361 ggtcgccagc aacgtggcgg cggcggtcgt gggcaaggtg gtggtcgcgg tcaacagcaa
    60421 ccacgccgtg gagaaggtgg cgcaaaatct gcaagcgcta aacctggaga aaaaccgtct
    60481 cgtcgcctcg gcgatgccaa accggcaggc gaacaacaac gtcgccgccg tccgcgtaaa
    60541 cctgccgctg cgcagtaatc ttttgatgcc gggctatgcc cggcaactga gcctgatggc
    60601 aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta catgaactct gcaatatatt
    60661 gaatttgcat gcttttgtag gccggataag gcgtttgcgc cgcatccggc atttcacaac
    60721 aagcacttgt cagcaatttg agaacgcggc aaaaagattt ctctgtttta tcgcccataa
    60781 gtcacccgag atgttacttc tctatagcca attgttcacg caactccatt aaaaggtagc
    60841 ccagtcgatt cttgccctta ccattaccac cgtctcccca gtaagcatca ttttccgtat
    60901 gctcaaccag ttttgcggac gcggttgcca gcaagagcgc acgcagttct gcatgttgtt
    60961 cgaatttagc gcgaagcgct tttcgcatca cctgttcttt gaccgactcc cagtttttac
    61021 gaagtggctt ggaacgatca cgccccatgc gagcggcgac cataggggaa gaaacccgac
    61081 ggatctcttc gcggtatttt tcatcaagga atttttgtgc ctggaaatag tgttctgagg
    61141 tgggccaggt tttcccgtca accttaatgg gccaggcggc aaagttagaa aaatcaccgt
    61201 agtcatcgcc ggtgctgtag aaatttatgg tggtgtcttg catgacgtgt tggatccttt
    61261 gtgctcgaac gggcattaaa ccgcattatg ttggtggtta ttgcgagtcg ctttccagaa
    61321 acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg tttatacagt
    61381 atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat
    61441 aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg gcagatgatt
    61501 gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat tgaagctccc
    61561 accggcgttg ggaaaacgct ctcctatttg atccccggca tcgccattgc ccgcgaagag
    61621 caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat ttacagcaaa
    61681 gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc ttttgggcgt
    61741 gggcgctacg tttgcccgcg taatctgacg gcgcttgcca gtactgaacc cacgcaacag
    61801 gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga gcaaaaacgt
    61861 tgtgcgaaac ttaagggcga tctcgatact tataaatggg atggtctgcg cgatcatacg
    61921 gatattgcta tagatgacga tctctggcgt cgtttaagta ccgacaaagc cagctgcctc
    61981 aaccgcaact gttattacta tcgcgaatgc ccgttttttg tcgctcgtcg ggagattcag
    62041 gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcc
    62101 gtattgcctg acccgaaaaa tttactgctg gtgctggacg aaggtcatca cctgccggat
    62161 gtggcgcggg atgcgctgga gatgagcgcc gaaatcaccg cgccctggta tcggctgcaa
    62221 ctggacttgt tcacgaaact ggtcgctacc tgtatggagc agtttcgccc gaagaccatc
    62281 ccaccgctgg cgatccccga gcgtttgaat gcgcattgtg aagagttgta tgagcttatc
    62341 gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc agaacaccgt
    62401 tttgcgatgg gcgtactacc tgatgaagtg ctggagatct gccagcggct ggcaaaactc
    62461 accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga gaaaaccggc
    62521 agccatgaca ttgtacgtct gcatcggttg attttgcaga tgaaccgcgc gctggggatg
    62581 ttcgaggcgc aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc cggtgcgccg
    62641 gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacatctctg gtttcactgt
    62701 gtgggtattc gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat tccgcacatt
    62761 attatcactt ccgcgacctt acgttcgctg aacagttttt cgcgtttgca ggagatgagc
    62821 gggctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt taaccactgc
    62881 gaacagggca aaattgttat tccccggatg cgcgttgagc cttccatcga caacgaagag
    62941 cagcacattg ctgaaatggc agcctttttc cgtgagcagg tggagagcaa aaaacatctc
    63001 ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc ggtttctcga ctatgtgacg
    63061 gatttacgcc tgatgttgct ggtgcagggc gatcagccgc gttatcgctt agttgaactg
    63121 caccgcaaac gcgttgccaa cggtgagcgt agtgtgctgg tgggcttaca gtcctttgcc
    63181 gaaggacttg atttgaaagg tgatctgctc agccaggtgc atatccacaa aatcgctttt
    63241 ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag cctcaaccgc
    63301 tatccgtttg aggtgcaaag cctgccgagt gcctcgttta acctgattca gcaggttggg
    63361 cgactgattc gaagccacgg ttgctggggc gaagtggtga tttacgataa acgcttgctc
    63421 accaaaaact acggcaagcg gctactggat gcattaccgg tatttccgat agagcaaccg
    63481 gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg
    63541 cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat cattaaagag
    63601 atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg cggcctgtat
    63661 gcccatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt gattgcgctg
    63721 cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat gcaaaatcac
    63781 accatcaagc tgacgccgcc tgcagacaag ccgatgccga ttgtcatccc cagttacaac
    63841 ggcgcgcgta aacaggccaa cctgacgccg ttgttggcga ttctcctgca caaactcggt
    63901 tttcctgtgg tggttcacgg ggttagcgaa gatccaaccc gcgtgctgac agaaaccatt
    63961 ttcgaactga tgggtattac gccaacgttg cacggcgggc aggcgcaggc gaagctcgac
    64021 gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga aaaacaactg
    64081 gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa actggcgacg
    64141 ccatttgccg aaggtgaggc actgcgtctt tccagcgttt cgcatccgga atacattgga
    64201 cgcgtcgcga agttctttag cgatatcggc gggcgggcgt tactaatgca tggtactgaa
    64261 ggtgaagtgt atgctaatcc gcaacgctgc ccgcagatca atttgatcga ccatgaaggg
    64321 atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttactgcc gcaagcaaaa
    64381 gatccggaaa ccacggcgca gtggattgag cgttgcctgg ctggcagcga accgattccc
    64441 gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggggaagc ggcaactatc
    64501 agcgatggcc tggcgcgcgt taatcaggca ttttaatttt tatctcagcc ggatgagcca
    64561 cgtcatccgg cttttacccc accgtaatat gaaattcctg catctttatt gaccttcccg
    64621 cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag ttaaccatgg
    64681 aaagtggtca tcgctttgat gctcagacgt tgcacagttt tattcaggct gtatttcgtc
    64741 agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaata gcggcaaacc
    64801 tggcagggca tgattcacat ggtattggca tgatcccaag ctatgtgcgt tcctggagtc
    64861 aggggcatct gcgaattaac caccatgcca aaatcgttaa agaggcgggg gcggcggtca
    64921 cgctcgatgg cgatcgcgca tttggtcagg tcgcagcaca cgaagcgatg gcgctgggga
    64981 ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg catcatatcg
    65041 gccgtatcgg ttactgggcg gagcagtgtg cagcagcggg gtttgtctct atccactttg
    65101 ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc cgctttggca
    65161 ccaatccgtt ctgtgtggtt ttccctcgta aagataattt cccgctgttg cttgattacg
    65221 ccaccagcgc catcgcattt ggcaaaaccc gcgtcgcctg gcataaaggc atccccgtgc
    65281 cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg gtaatgcagg
    65341 agtcgccgtt gggttcgctg ttgacctttg ccgaacataa aggctacgcc cttgcagcaa
    65401 tgtgtgaaat tcttggtggg gcgctttctg gcggtaaaac gacgcatcag gaaacgttgc
    65461 aaaccagccc cgatgccatt cttaactgca tgaccaccat catcatcaat ccggagctgt
    65521 tcggcgcgcc ggattgtagc gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc
    65581 cgcatgatga cgataagccg attttgctac cgggcgagtg ggaagttaac acgcgtctcg
    65641 aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt tgtgatgcgg
    65701 cgcggcagat tggtatgccg gaagagacgt tgcaggcttt ctgtcagcag ttagccagct
    65761 aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat caatgagggt
    65821 tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga cgtagcgtaa
    65881 acgcctgatc cagccaacgg ttcggatttt gtaggtctga taagacgcgg tagcgtcgca
    65941 tcaggcatct gttgccggat gcggcgtaaa cgccttatcc gtcctacgaa ttacttataa
    66001 attaccgcag taccacttaa tttattggtg ttggtggcag aagtgatgct gtatccacta
    66061 gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg
    66121 ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc cgttaccggt
    66181 tccgcggcaa acacgccaaa tgacaaagtt gacagagcca tagcagcaac aacagtattg
    66241 atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt gtttcgtcga
    66301 tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga ccgccttgtt
    66361 caaataaatt gattgatatc taacatactg ataacccatt tatttgttga acttatggac
    66421 ttgttttatg cagcgtggca gtcacgacag cgttaaaggc acggaatgcc acgcaaaata
    66481 caaattatac tctctataca tcccgccagt tgtagttaac gtaactggct gtctttcgaa
    66541 cctctgcgat tatatcctgt ataatctggt ttttgtaaat ctttctcctg ctgacaatga
    66601 atgcataagc gcacaccagg aatggcttcc cgacgggctt gcgggatggg ggctccgcac
    66661 tcttcacatt catacaggct ttcaccacgc ggaatttcac cccgagcgcg ggcaatcgca
    66721 tcttcaattg tactgttgat ctgttcgttg acggcgtcat cgttagccca accggatgcc
    66781 atatcgtcct ccccatatca atacttgtac agttaagtgt agctaatata gggacgaact
    66841 cgggcagttc aagcgtcaga tctccgacca ttcccgcagc agattatgat aaagattgag
    66901 cagcgacagg atctcttcac tttcgccgta gcggcttttc agcgactgaa tattgttgtc
    66961 cagatcgaac agcatggcgc gctttttatc atcgcggatc atcgactgga tccacataaa
    67021 tgatgccact cgtacgccgc gggttacggg cgtcacgcaa tgcaggctgc tggagggata
    67081 caacaccaga tcgcctgccg gaagttttac ccgatgttgc ccgaaggtgt cattaacgac
    67141 cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag aaaggtcagt
    67201 gcgcatccag ccgttttgtg ggtgactgcg tactgcgcca tccacatgaa aaccataagt
    67261 ttcattgttc tgatagcgat taaacagcgg cgtggaaagg gtatgcggca aggcagcggc
    67321 aaagaacaat gcatgttggt taaccgcgtt cagcacctca ttttgcagtg cggcgtataa
    67381 cgcgctgcgg gtgtctacct gttgattgtt cttaacttgc gcaccttgtg cgccggtggt
    67441 gacgcgtcca tccacccatt cggcttgttc cagttgttcg cgaaaacggg cgacgtcctt
    67501 tggcgatagc acgccgggaa tgtggtacat catacaagtt ctccaaaaag tggggcctgc
    67561 gccccacatc tgaatcagaa atgcatattg gccgtgagca agaaggttct tggctcgccc
    67621 gggtgataac ggtagccgct cttgttgatt gaggcgacgt aatcggtatc aaacaggttg
    67681 tagacgttta gctggaagtc gagattgcga ttaacccgat accccagttt agcgtcggcg
    67741 acccaataac cttcggtaaa cgctggcgtt cccaccgcgc cgtcagaacc tttatgcata
    67801 ctgccgatat agcgtgcgcc cgcaccaaca gagatatcat cggttgcctg atactggctc
    67861 cataaggtaa aggcgtgctc cggggtatac ggcagcgatg aggaaccatc ctgggcaaca
    67921 tctttgccgt ttttgatggt tgctttttgc tgggtatagc cgccaatcac ctgccacgcg
    67981 ggagtgatat tcccggccac ggatatctca tagccttcga cgcgtttctt accgtattgc
    68041 gagtaagttc cgtcatcatt ttgctcaact tcattttcga tatcagtacg gaacagcgcg
    68101 gcggtgagca acaggcgttt atccagaacc tgccatttgg tgccaatctc gctggtgttg
    68161 gctttttgtg gtttaaaatc ggtgcggttg gcactgttac cgctgccaga ctgcgcaagg
    68221 gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat agttaatata gacattgccg
    68281 ttttccgtca ggtgatacag cgccccggct ttccagttca ccagattgcc cgacttagcg
    68341 gtatcgacgg tggtgaccgg agaacctttt gccatgccag cagggcaggt gatggcaccg
    68401 cgtccgctgc cgccgcaggc ggtggcactg tcatattcag tatgataatt atccagacgg
    68461 atcccgccgt tcagctcaaa atcacgggtg atttgcagcg tatcgaaggc gtaaattgcg
    68521 aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg tcaggccgcc gggatgaatg
    68581 ctgctgtcag gatgataaat atttaccgca ggtaacgtca ccggattaac gccgtagtta
    68641 gtctgcgttt cacgggtaaa ttccacgccg gtactgacat catgaccgat agaaccggta
    68701 tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt tattactcac atctttagta
    68761 ttcgccgtgc gtgaccaggt ccagctattc acatcgctgg tgggctgggt aatattcgac
    68821 gccccgccca taatcgccgt catcaggtaa tcctgcttca cgcgcgacca acgggtagta
    68881 ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac gcatagtagc ggtgtcggtg
    68941 gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat gagtatcaac ttttccggaa
    69001 tgattcagag ccgctgttcc cgcagatggg gcagaataac ccggtaaacc gatggtcgga
    69061 atgccgccgt ctggcgtgtt gtgctgggtg acatgcagat aattaagata caaacgattc
    69121 gctgtaccaa ggccaaaagc gacagaaggg gcgacgccgt aacgctcatt tttgattttg
    69181 tcgcgtccgg catcgtgcgt tttttcgccc atcacgttca ggcgcaccgc agtggtatca
    69241 ccaatgacct gattgacgtc cagcgtgccg cggcggaacc aggcgctgcc aatactcgcg
    69301 gaggcgtcaa tgcccgaatc attgcgcggc tgcttgctga tcatattgat cgagcccgtc
    69361 ggtgcgctgc gcccgtagtc ggtgccggac ggccctttaa tcacttcgac ctgctcggta
    69421 ttgaaggtgt cgcgcgagac gctgccgata tcgcgaatgc catcgatata aatactgtta
    69481 gaggtatcgg caccgcgcat ataaatggcg tcgccagtgg tggagttacc gttctcaccc
    69541 gcaaaaaacg cacccacgcc ggggacgttt ttgagcgcgt cggtaaggtt ggttgcgccc
    69601 tgatctttaa tcacttgttc agaaatcacc gtcatcgtgc gggtagtatc cgctaccgga
    69661 cgcgagaatt tcggatcggc agattgttgt ggcgcataaa gcgaaggcgt tgatgcttcg
    69721 acaaccagcg tgtcatccgc gtttgcttgc ccttcggcgg cgagtgcctg agccacaggc
    69781 gtgatgccaa tacaaagacc ggcaaagaac gtgagcgaat gaaattgtct ggcagggaaa
    69841 ttgcgattgt tttccatttt gcaggtgact ttttcttata tgttgaaatt ttcactgcca
    69901 cgagaaagcc tctctgcgga tgcgatgcca ctgttaatct cgtgatagcg ttcgtgcaag
    69961 tgataataat tttgataagc attatcactt ggcgcgattt tctatcaccc attgcgcgaa
    70021 aaagaaatac atttatttac attgcgttca tatttgatgt tttttgatta caaaacaggc
    70081 gaaaactgca tccagaggcg aatgcagtta cgttggtggg gttatttgta gatggttgca
    70141 gtaccgaaca tctggtcatt tccgcccgcg gaattaatga cataaccttt cgcgccttgc
    70201 tcgcgcgctt tttctgccag tttatcctcc agatcgctta ggtttgatgc gccagttgcc
    70261 gaaacggtac cggcgggccg tagctgaccg ctgtccaggt tactcatcgg ttgcgcggca
    70321 taagccgtga gagagattcc gctcaggacg gtggcaatca gtagtgtgag gcactttttc
    70381 ataatgacat cctcataaat acaacgggta gtgcctgata agtgtaggtc gcctgggcga
    70441 tgaaaatgac gcaaaaaatt gacagagatg cgcttgtttt ttgaacgata tatttttaca
    70501 aaacaagaca aatcaatgac atgcataatg atgattttta tgatgaattg ccataaacca
    70561 tagccatgtg aaggtttata gaaatgataa acgcagccag atatttaccc tgtcgcgtta
    70621 tgcgggtatc ttacgccgct gcttaaagga gaatgctatg tccgcccaga aaccggggtt
    70681 gcatccgcgc aaccgtcatc acagccgcta cgatctcgcc acgctttgtc aggtcaatcc
    70741 tgaactcagg caattcctca cgcttacacc cgccggggag caaagcgtag actttgccaa
    70801 tccgctggcg gtgaaggcgc tcaataaggc gttgctggcc catttttacg ccgtagcgaa
    70861 ctgggatatc cccgacggtt ttctctgccc accagtaccg ggccgggcgg attatattca
    70921 tcaccttgcc gatttactgg cagaagcaac aggatcaatt ccagcgaatg ccagcattct
    70981 ggatatcggt gttggtgcga actgtattta cccgctgatt ggcgtacatg aatatggctg
    71041 gcgttttacc ggtagtgaaa ccagcagcca ggcgttaagc agtgcgcagg cgatcatcag
    71101 tgctaatccg gggcttaacc gcgccattcg tctgcgtcgg caaaaagaga gtggggcgat
    71161 ttttaacggc atcatccata aaaacgagca atacgacgcg accttgtgta acccgccatt
    71221 ccacgattcc gccgctgcgg cacgggcagg tagtgaacgt aaacgccgta acttggggct
    71281 gaacaaagac gatgtactga actttggtgg tcagcaacag gagttgtggt gtgaaggcgg
    71341 tgaagtcgct tttatcaaaa agatgattga agagagcaaa ggcttcgcga agcaggtgat
    71401 gtggtttacc tcactggtat ctcgtggtga aaacttaccg ccattgtatc gtgccctgac
    71461 ggacgttggc gcggtgaagg tggttaaaaa agagatggcc caggggcaaa agcagagtcg
    71521 ctttattgcc tggaccttta tgaacgacga gcagcgccgc cgttttgtca atcgccaacg
    71581 ttaaagcgtt ggctcccccg gcggcagcgg ttcagccggg gccgcgcccg gagcaggcag
    71641 cacctgataa gtctgtaccg gcgcgcgaac acccgccagg tcgaaatgtt ttttcacctg
    71701 gctgtcgagg gcaaagcgga ccgtccactg tttgagtggc agcgtggtga acgaaacacg
    71761 cagtgtaaat gcggtattgc ttaagccgac aatcccggca aagttcggtt caccgataat
    71821 caacccgcga atttcttcgt tttccatcaa ttcagctacc gcatctttca gtgcctgatt
    71881 ggctttatca gcatcttcat ggcgatcaac atcgtaattt gccaccaccg aaccaatgcc
    71941 gcggacgaag ttagcaaagg tggttatcga agaccacgga atgatgtgat acgccccggt
    72001 atcctgacgc acgcccacgg agcgaatcga catccgttcc actgtgccgg tcaacggccc
    72061 gatagtcacc aaatctccag tgttcatgcc gttttcaaac tgaataaata cgccggtgat
    72121 aatatctttc accagcgttt gcgaaccaaa agagatagcc agccccaatg caccggcacc
    72181 tgccagcaat ggtgcgatat tgacgccgat ttccgacaac acaatcatga tggtgatggt
    72241 actgataatc accgccagcg cgttacggaa cagcgtcagc agggtacgcg tacgggcgct
    72301 gggtagcggg cggccatgaa tatccgaagc cagccggttt tcgatcaaac tggcgagcac
    72361 cgtccagcca accgccgaga agaaaagaat gagtgcgata cggatcagga tatctacggt
    72421 tttctgcccc gcaccgtttt gcagccagtt ccagaaatcg aacaatcccc atgcgctcaa
    72481 cagcaacatt accgccacgc agacggtcag aatacgcgcc gttttcagcg ccgccgacag
    72541 ccagccattc aaccgttttt gcagctccgg atagttacgc tgagtatgtg gcgagagagt
    72601 gatggttttc gccagccagc gggaaaacat accggaaaca aatgctgcga taccaataat
    72661 cgccaggctg cgcaccgttg cacccatcat aaatttcagg ctgttgcccg gatcgaacaa
    72721 cgaaaagaaa aacagcacga taaaataggc gcttgccagc cagtgccaca ccagcgcaaa
    72781 ggcgcggata aacaggctga aaaaggccag cgaatgctcc gcgaagttga gcaaatgctg
    72841 ggtaatctct tttttattac gaaagatcag gtacaacgcc cagaccgtca tgcacagcat
    72901 aatgatgacg ttcgccagcg cacctatctg tacattcacc tgattagaga taatcggcac
    72961 ggccacaatc aggccatagc caatcaggct gcttaaccag ctcaggcgac gactccagta
    73021 acgggcgctc tcgtcctgaa tcgtgaacgg gcgcagctcc gccacgtttg ggcaaaaaat
    73081 caggcgtagt acagctttga aaaattcaat cagggcaaag gcgttgagaa acaaactttg
    73141 ttggaaagcg atggtgcgac tgcccgcatt caggttgtcg cttaaaacct gaccgacaaa
    73201 taatgtcagt gccagtaaca gcaggtcgat aataaacgcc ccgataatca tcgccggaag
    73261 ctgcaaccag ttactgcgct cacgattttt ttgccgcgcc cactggccca ttttgcgata
    73321 cagcggaagt gcgcacaggc gaatcagcca gtaaaaacca aacactaata ccgctaacat
    73381 tgaaaaatgg gtcagcgcat tgctgaaggt ttgtggatta aacggcttat gcggggagcc
    73441 ggtgatattg cgataaagtt gcccgaagcg ggcggaaagg gcttcaccat aatggcggct
    73501 gacttcggtg accttttgca gcacggtttg ctcttcaacc agcgtcggcg gcacgatctt
    73561 tggtaccggt tccgcagggg gcgtagcggc gacggtgcgc aactggtcga tcaattcttt
    73621 acgcgaggtg tcattatcca gcacatccgc cagtgcgcca taggccgctt ttttttgttc
    73681 gatatccggt tccggggccg gttcagtcgt tgagtccgtt gtcgttgtgg ttgtaacgcc
    73741 gggtatggat accgcgtggg caggtgcgcc cagcaggcag aagaggatga acaggatcca
    73801 ccgcatgact cctccagtga gaaaacagag caaaaagata agtatagatg ctgggggagg
    73861 ggcgatttta aatgagagga atctggtgtg cctccctttc gggggaaagg gaggaaggga
    73921 tattaagcag gttgctgaca acgtgcgggc ttttttgccg gatgcggcgt gaacgcctta
    73981 tccgtcctac aagttcatgc aattttaata agttgccaga tatcctgtag gcctgataag
    74041 cgtagcgcat caggcaatgt gtcttattaa gagacgtgct gcaaaaattc ctgcaagcgc
    74101 tggctcggcg ggttcttgat caacacctgc ggatcgccat cttccgcaat ccggcctttg
    74161 tcgataaaga taaggcgcga agctactttc tcggcaaaac cgatttcgtg ggtcacgatc
    74221 accatcgtca tcccttcttc agccagatcc tgcataacct tcagcacttc atggcgcagt
    74281 tccgggtcaa gagcggaagt cggttcatca aacagcatca ttttcggctt caccgccagc
    74341 gcgcgggcga tggccacacg ctgctgttga ccaccagaaa gttcggaagg gtagtgatgt
    74401 gcacgttctg ccagaccgac tttcgccagc agctcacgcg ccagtttttc cgcctcttct
    74461 ttgttcgcgc cacgcacgcg tagcggacca aacatcacgt tttccagcgc cgtcagatgc
    74521 gggaagaggt aaaactgctg gaacaccata cccgcttcct ggcgaatcag gcgctcgtca
    74581 actttcggat cgttaacctt caggccatcg acaatcagat cgccggaggt gatttcttcc
    74641 agtttgttga tgcagcgcag cagggtcgat ttaccggaac cggacggccc gataatcacc
    74701 acgacttcgc cctgagcaat gttcaaatcg atattgtgca gcacctgggt tggaccaaaa
    74761 tgcttggaga cgtttttaaa ttcaatcaca ggattttcat ccttctttcc agacgacgca
    74821 gaataaagct cagcaccagg gtaataatca gatagaacac cgccacggcg ctccagatct
    74881 caagggcgcg gaagttaccg gcaataattt cttgcccctg acgggtcagt tccgccacgc
    74941 cgatcacaat aaacagcgag gtgtctttaa tgctgatgat ccactggtta cccagcggcg
    75001 gcagcatacg acgcagtgcc agcggtaaaa tgacgtagcg aatggtttcc caacgtgaaa
    75061 gaccgagcgc cagtcctgct tcacgaaaac ctttgtgaat agacagcacc gcaccacgcg
    75121 tgatttccgc aatgtacgcg ccggagttga tcatgatggt gaccaccgcc gcagtaaacg
    75181 ggtcgatgcg taagtcgtta aacgccatcg gcagggcgaa atagataaac atcacctgga
    75241 cgacgatagg tgtgccgcgg atcacttcaa taaagaccag cgcgacgtgg ttggctatcc
    75301 aaccaccgaa ggtgcgtgca aaacctgcca gcaatccgat caccaggccg ccagccagac
    75361 cgaggaccga gatccacagg gtcattttgg caccttcaat cagaagcgga atggcaggcc
    75421 agatggcact ccagtcaaac tgcatatgtt gttcctgtta ccgtggtgta aataatcaaa
    75481 ttcagggtaa ttaatggccc cggagcgggt ttgcgcccct caccctaatc ctctccccag
    75541 aggggagagg gaactgccag tgcgccttac aggcgtagcg ttattatttc ggttcagtac
    75601 cgaaccattt tttgtagatt tcgttgtaag ttccgttctc gcgcagggtt ttcaacgcgc
    75661 cgttgacttt gtcacgcagc tcgtcgctac ctttcgggaa cgcaataccg tattgctgtg
    75721 cttccagaga gtcacctacc gctttgaact gaccgttacc ggcggttttg atgaagtaca
    75781 gaatgtttgg cgtatcgtgc agaacggcat ctgcgcggtt ggtgcccagt tccatatagg
    75841 cgttatcaat gttcgggaac tgacgcagat ctttagtttt gatgtttgct ttcgcgtaat
    75901 caacggagcc agtgccgctc ttcaccgcaa ccactttccc gtcgagatct ttcacgcttt
    75961 tcacatcgtt attgttagct ttcaccatca ctaacaggcc gcttttgtag tagccgtcag
    76021 agaaatcgat cgctttttta cgctcgtcgg taatggtgat gcccgccagc gccagatcga
    76081 cgtttttggt ttgcagtgcc ggaatgatcc cactgaaatc catcggcttc agttcgtaat
    76141 ccagcttcag ctctttagcg atggcagccc acagatcaac gtcaaagccc acatatttat
    76201 cgccctgttt aaattcaaac ggaacgaagg cggtatccgt cgcgacaact aattttttat
    76261 ccgcggcatg agaagaaacc gcaaaagcca gggtcagtgc agccagtgaa acttttaata
    76321 cagacttcat agcatttcct tttttctatc cacgggacga tcccctgcgt gacattcaca
    76381 tatatgaaaa aatcgtgcca gttttgcaac tcattgattt atcgagatgc ggtaaaaacg
    76441 tgatgcacga tttatagggc aataccctga agatgcacca ttctggggcg ccaaactggt
    76501 gcgctaaaat tgtgcactca acacagtgat tatttagcgt aaaacctgat gaaaaaacaa
    76561 tctttatgta acgattgtgt gatgatgtgg atacaaaaaa tttacttaat cagctggaga
    76621 tagcagatgg atgcactaaa taagtgcgtt gaggcgggct gcaaaacaaa acggcctcct
    76681 gtcaggaagc cgcttttatc gggtactaaa gttctgcacc atcagcgatg gatttattcg
    76741 atgttagact cgataaacca caggaattta tccaggtcgc gagacgcggc ggtcaggata
    76801 tctgcggtgt cgtcatcttt cgcttcgcca atcgctttgc gtacgtcatt agcgacgatt
    76861 gcgtaacggt cagccagctc tttcaggtga tcctgaacgt tgtggatgtc cagcgggtaa
    76921 cttttcagcg gagttttgct gttgataact tgagtggtcc ccagagctac accgcccagc
    76981 tgcactgcac gttctgccat ggtatccaga tgatcgatca gtgcggtgcg gaagccatcc
    77041 agcatttcat gtacggcaat gaagttagcg ccgcgcatgt tccagtgcgc ttgtttggta
    77101 atcaaagaaa gatcaataaa ctggataacc tggcgattca gcaactctac tgttgctttt
    77161 ttctcgctgt cggagacatc gttgcgggta taaagcagat tggtcgcttt tgatttaact
    77221 aatttagcgg tactcataat ttcatatcct cttgatgtta tgtcccagta attaacgaga
    77281 ctaagtatag caccggctat gtgttccgct attctggctg ttcctatcac actaatagtg
    77341 gtaacaagcg tgaaaaacaa aactaataaa aattcaatgg gttagttata ttgataagaa
    77401 caattcttat ttattggctc gccaggaatt ttcggaataa atatgctttt gcataattcg
    77461 atatataaag acggtgtaga ggaaaagtag cgagaaattc tgcatggtta tgcataacca
    77521 tgcagaaatg taaatttaat taatgtctaa ttcttttatt ttgctctctt tgcgtactgt
    77581 cagcgtagac cccattgaag cggcgatgat agcgccgagc gccagcagct gtatgggtgt
    77641 cagtgtttct ccgaggaaaa tcatcccgga aacggcagcc agcgccggtt ccatgctcat
    77701 cagcgtacca aatgtccgtg ttggcaaacg ggtgagggca atcatttcca gcgaataagg
    77761 cagagcggtc gagagaatag cgacagccag acccaatgga ataaccgacc agtgccagag
    77821 tgcttcacca gcctgaagcg ctccaattgg cacgaaaatt aacgctgcaa tcaacgaacc
    77881 aattgccacc gtcgcagggc catgttccgc tcctgcgcgt tgcccactta aaatgtaaat
    77941 agcccaacaa gccccggccc ccagagccag cgcacagccg gttaaatcga catgggaaac
    78001 gtcttgcccc agcggtagca ggaaccacag accaagaacc gccagaacaa cccagacgaa
    78061 atctaccggg cgacgagaag agaacagcgc caccgccagt ggtccggtga actccagcgc
    78121 caccgcaata cccagcggta ccgtctgaat agaaagataa aaaagataat tcatcccccc
    78181 cagcgaaacg ccgtaaaaca acagcggtaa ccgctgctct ttggcaaagc gcagtcgcca
    78241 tggcttaaag aacgcgatga ggagcagcgt tcctaatgcc agacgcagcg cagtgacacc
    78301 cggtgcgccc accagaggaa aaagtgactt agctaacgag gctccaccct gaatagacgc
    78361 catggcaacg agcaatatga ctattggtaa ccagaccggc attttacgta atgaaccagg
    78421 catcctttct cccacaaata tctagactta agtaaagcgt ggagtgtact ggatataccc
    78481 aatgctggtt gagcatttgt tgaaaaaatt ttcccccgtt ttgactaaaa tgcgccagga
    78541 ttgatggaat cattagtctg gtgattagga ataatctgga tgaatgacag ggaaaacatg
    78601 cgtaatactt acgcagttct ctgaaaaagt gatttaaatt tagatggata gcggtgtatg
    78661 gaaacgttct gttacatgaa atggcccgtt agacatcaca aatcgcgaag agtttcccat
    78721 taatttttga tatatttaaa acttaggact tatttgaatc acatttgagg tggttatgaa
    78781 aaaaattgca tgtctttcag cactggccgc agttctggct ttcaccgcag gtacttccgt
    78841 agctgcaact tctaccgtaa ctggcggtta cgcacagagc gacgctcagg gccaaatgaa
    78901 caaaatgggc ggtttcaacc tgaaataccg ctatgaagaa gacaacagcc cgctgggtgt
    78961 gatcggttct ttcacttaca ccgagaaaag ccgtactgca agctctggtg actacaacaa
    79021 aaaccagtac tacggcatca ctgctggtcc ggcttaccgc attaacgact gggcaagcat
    79081 ctacggtgta gtgggtgtgg gttatggtaa attccagacc actgaatacc cgacttacaa
    79141 acacgacacc agcgactacg gtttctccta cggtgctggt ctgcagttca acccgatgga
    79201 aaacgttgct ctggacttct cttacgagca gagccgtatt cgtagcgttg acgtaggcac
    79261 ctggattgcc ggtgttggtt accgcttcta atcacttcgg tgatataaaa aatccgcctc
    79321 tcggggcgga tttttgtttt taaggtttcg ggtcgaaaat atcggttccg agatggttgt
    79381 aatccacctt ctgtaactgg aagttggtga tgtatatcgg cccggctttc ttctcagata
    79441 taaacgggta tttgatgtta atttccttcg ctttaattcc cgtccactgc gagaaaaagc
    79501 ctaagaagtc attggctgag cggcgggctt taatcacccg atgcgcttta tcgtcgctgg
    79561 aaatgaccat aaaaggcacc tggaaatttt gctgatactt atcatcatgg gcaaggtatt
    79621 gcacgtcttt gccgcgctct ttaaaagcca gaccgtggtc agaaaagtaa accagcgaga
    79681 agctgctgcc gctgttgcgt aactgatcgt acagcttgcg cagtaaatcg tctgtttgcg
    79741 tcatggtata gagatagcac gacgtttctt tcgattgcac aaaggtttcg tatttgcctt
    79801 gtgtccggtc gcaggcctgc ggatgtgagc ccatcagatg taaaacaatc agctgcggtt
    79861 gcgagtgctc ttgcgccagc acttgagcgg tcatatccag taacgcttcg tctttcgtgt
    79921 ttttatctgc ttcaaaatta ccttctttca ggaagtacac ttcatctgca cgtttggcga
    79981 tgctggcgat ggcggtatcg tattcgccga tttggccctg gttggaaaac caccaggtct
    80041 ggaagcccgc gcgatttgcc agggtgacaa agttatcctg aaactgtggt ttgccatcga
    80101 ctacgcgatt gagcgttaag ccaagcgatt tctgcgtgga gccactggcg gcaatgtagt
    80161 cagcaaatat caaaccgtta acactgctgg caaacggggt attgttccag tgaccgccaa
    80221 aggcaccgag ggcatcgcga cgcgcgcttt caccgatcac caccacatag gtctgatatt
    80281 tcggcttaac ggcagtgacc gtccaggtat ctttcattcc ggaaagtttt gccatccgtt
    80341 cttgctcttc aatcacctcg ttattattga cgatgacatc cttagcaaaa cgaaataccg
    80401 gatagccagt atctttcagt ttaaatacgc cgccccaggc caggttttgc atgggagcaa
    80461 caaaaaatgt cgccacactg aataccagac acaagctgtc gaaggtattc cagcgcgctt
    80521 tttcactctc tttttttcgc ctgattgcta ttacgccgag cgcaaaaata aataagccga
    80581 ccaggtagct gtaccacgga aaaatcgtca ggatttcggt cgactcttcc atattggtgg
    80641 agtgcaatgc cagcaatgta ttgaaattag gcgcgccgta ggcctgagca aaagggaaat
    80701 aacaagccgc caccagcgaa ctgacaccga ccagaacttt ttgtacgcca ggcaatgttc
    80761 gccatagcaa aagcaaaata gccgtaaatg cggcggtgta gagcaaactg aaggggtaac
    80821 ctaagccgag gttaattaat agcgactgta aaaagtagaa cgcagtccac gggctaaata
    80881 cccggctacg ggtaacaagc gattctttga gggttaaatt catatgccac tatcgtaaca
    80941 aacgccatgt gcttaccctg gcgtcaaggg tcaatacctg caagagtgcg tgaagagggg
    81001 aaaggggtcc gcatccgcga gccgcaatat acgcagggct gcaagaagat agagcgagct
    81061 gccgttcagg tcaactactg acgaacaaat cttttgcgaa ggggattgca aatccgacaa
    81121 aaagtggggt ttttcagggc aaaacgcgtt gtgcctgcat aaaagcagga gtgaagcggc
    81181 gtaccgcgac gccgctattt atcggaaggt ttatcttgct gcggtttgtt gttgaccata
    81241 tcgcacaaca tagagagcag cattaaccgt actttaaagg gagaatgact aaacacgcgc
    81301 atacacctct tgaactcatt cataagacct cctgacttgc taatcccgtc gatccttgag
    81361 ggatgattgc attacataca gatatagcac aggctatatt atatagctat tgctaaaacg
    81421 ttaatttttt gtaccttcgc aactctggtt tacaatgtgc gcacgaaatg agaacgctag
    81481 gtatgcgtca ccataatgag gaagcacaat gagtcgtcgc gcaggtacgc caacagcaaa
    81541 aaaagtgacg cagttagtga acgtggaaga gcacgttgaa gggttccgcc aggtcagaga
    81601 ggcgcatcgg cgcgagctta ttgatgatta cgttgagctg atttctgact tgatcaggga
    81661 agtgggggaa gctcgtcagg tagacatggc tgctcgtttg ggagtttcgc aaccgacggt
    81721 ggctaaaatg cttaagcgac tggcaaccat ggggctgatt gaaatgatcc cctggcgtgg
    81781 cgtgttttta acggcagaag gagagaagct ggcgcaggaa agccgcgagc gacatcagat
    81841 agtcgaaaat ttcttgttgg tgttgggcgt cagtccggaa attgcccgtc gcgacgcgga
    81901 aggcatggag caccatgtta gtgaagagac gctcgacgct tttcgtttgt ttacccagaa
    81961 acacggtgcc aaatgagcct gcctttttta cgcacgctgc aaggcgatcg tttttttcag
    82021 ttattaattc ttgttggcgt cggattaagt ttttttgttc cctttgcacc gaaatcctgg
    82081 cctgctgcta tcgactggca caccatcatc accttaagcg gcctgatgct gctgactaaa
    82141 ggtgtggagc taagcggtta ttttgatgtg ctggggcgca aaatggtgcg ccgctttgct
    82201 acggagcgtc ggctggcgat gtttatggtg ctggcggcgg cgctgctttc tacctttctg
    82261 accaacgatg tcgcgctgtt tattgttgtt ccgctgacta tcacgctaaa aagactgtgt
    82321 gagatcccgg ttaatcggct gattattttt gaagcgctgg cagtcaacgc tggttcgcta
    82381 ctgacgccaa ttggcaaccc gcaaaatatt cttatctggg gacgttctgg tctttcgttt
    82441 gccggattta ttgcccaaat ggcaccactg gctggcgcaa tgatgctgac gctcctgctc
    82501 ctgtgctggt gttgtttccc tggaaaggca ctgcaatacc atacgggggt gcaaactccg
    82561 gagtggaaac cgcggctggt gtggagttgt ctggggctgt atatcgtctt tctgacggcg
    82621 ctggagttaa aacaagagct gtggggactg gtgattgtgg cggcgggctt tgcgctgctg
    82681 gcacgtcgcg tggtgttgag tgtggactgg acgctgctgc tggtgtttat ggcgatgttt
    82741 atcgacgtcc atttactaac ccaactgcca gctttgcagg gggtgttaag taacgtgagt
    82801 catttatccg aacccggatt atggttaacg gcaatcggtt tatcgcaggt gatcagtaac
    82861 gtgccgagta ccatattgtt gctgaactat gtgccgccgt cgttgctgct ggcatgggcg
    82921 gtaaacgtgg ggggctttgg tttattaccc ggatcgctgg caaatttgat tgcgttacgg
    82981 atggcgaacg atcgccgtat ctggtggcgt ttccatctct attcaatacc gatgctgttg
    83041 tgggcggcgc tggtgggata cgttttattc gttatgctcc cggcctggta gggtctggca
    83101 aataaaaaag gcggatgatg agtccgcctc tgttttacgt tttgacgatt aattcagacg
    83161 aaccggcatc ccggaacgat ttttgatcgc ttcatccaga acaacctggt caacatctgg
    83221 ctgaccggtc actgtctgca cgctcttcgt cagggtaatt ggcacaattt cctgaccttc
    83281 aaactgggct tcggtggtag acagcgggtt atggacttca atataacggc tgccgtctgg
    83341 ctcggtggtc gcttttaccg gctcatcaat aaactgtacg cgagtaccga ccggcacttt
    83401 ctcgaacagg aatttgatgt cttcgttacg cagacgcaca caaccatgac ttacgcgcag
    83461 gccgataccg aagttggcgt tggtgccatg gattgcgtac aggcgaccga tgtagagtgc
    83521 atacagaccc atcgggttat ccggacctgc cggaacgaca gccggaagcg gttcgcccgc
    83581 agcgcggtac tctgcgtgca ttttagcggt tggcgtccag gtcgggcctg ctttcttacg
    83641 ctcaactttg gtggtccagt tgataggcgt gtctttgcct aactgaccaa tgccgatcgg
    83701 cagcacgata acggtgttgg tccctttcgg atagtaatac agacgcatct ctgcactgtt
    83761 aatgacgatg ccttcatgaa cggtatccgg caggatcagc tgctgcggaa tgttcagtac
    83821 agtaccgcct ttcggcagga aggtatccac accggggttc gcttccatca tattggaaag
    83881 acccatctgg tactcggcgg caaaatactc cagaggctga gtgttacctt caggaatggt
    83941 gatcacctga ttctgaccaa ccaggcgact cccgtcggtt ggcagaggat aagttaccgc
    84001 agaggcggta ctgcaaaagc cgacaacagc gaaggccgct gcgaataatg ttttcaattt
    84061 catattcatg ttaagcgaga ttttgtgcct ggcaggccat tgggttgaga atattagagt
    84121 attggaagcg cattataagt tcattcctgc tcacagtgaa atcagatgtg tacgaaatca
    84181 cattttttgc ctttggcttg agtgtagacc ttaagcgagg agcaggatct tctttcagac
    84241 ttatggcata atgcgcggtt tgtcatatct cttttcagga tacgcctgtg ttagtttcca
    84301 gtaacgtcac catgcagttc ggcagtaagc cgttgtttga aaacatttcc gtcaaatttg
    84361 gcggcggcaa ccgttacggc ctgattggcg cgaacggtag tggtaaatcc acctttatga
    84421 agatcctcgg cggcgactta gagccgacgt tgggtaacgt ttccctcgat cccaacgagc
    84481 gcattggtaa gctgcgtcag gatcagtttg cctttgaaga gttcactgtg ctggatacgg
    84541 tgatcatggg gcataaagag ttgtgggaag tgaagcagga gcgtgaccgc atctatgctt
    84601 tgccggaaat gagtgaagaa gacggctata aagtggccga tctggaagtg aaatacggcg
    84661 aaatggacgg ttactctgcg gaagctcgcg ccggtgaact gttgcttggc gtgggaattc
    84721 cagtggaaca gcactacggc ccgatgagtg aagttgctcc tggctggaag ctgcgtgtgc
    84781 tgctggcgca ggcgctgttt gctgatccgg acattctcct gctcgacgaa ccgaccaaca
    84841 acctcgacat cgacaccatt cgttggctgg aacaggtgct gaacgagcgt gacagcacca
    84901 tgatcatcat ctcgcacgac cgtcacttcc ttaacatggt ttgtacccac atggctgatc
    84961 tggattacgg cgagctgcgc gtttatccag gtaactacga tgagtacatg acggcggcga
    85021 cccaggcgcg tgagcgtctg ctggctgata acgccaagaa gaaagcgcag attgctgagc
    85081 tgcaatcttt cgttagccgc tttagtgcca acgcctcgaa atctcgccag gcaacttcgc
    85141 gcgcgcgcca gattgataaa atcaaactgg aagaggtgaa agcctccagc cgtcagaacc
    85201 cgttcatccg ttttgaacag gataagaaac tgttccgtaa cgcgctggaa gtggaaggtc
    85261 tgaccaaagg gtttgataac ggtccgctgt ttaaaaatct caacctgctg ctggaggtcg
    85321 gtgaaaaact ggcggtactg ggtaccaacg gcgtcggtaa atcaacgctg ctgaaaacgc
    85381 tggtgggcga tctgcaaccg gacagcggca ccgtaaaatg gtctgaaaac gcacgcattg
    85441 gttactacgc ccaggaccat gaatatgagt tcgaaaatga tctgaccgtg ttcgaatgga
    85501 tgagccagtg gaagcaggaa ggcgatgacg agcaggcggt acgcagcatt ctcggtcgtt
    85561 tgctgtttag ccaggacgac atcaaaaagc cagcgaaagt gctttccggt ggagaaaaag
    85621 ggcggatgct gtttggtaaa ttaatgatgc agaagccgaa cattctgatc atggacgaac
    85681 cgaccaacca cctggatatg gaatctattg agtcgctgaa catggcgctg gaactgtatc
    85741 agggcacgct gatctttgtt tcacacgacc gtgagttcgt aagctccctg gcgacccgta
    85801 ttctggaaat caccccggaa cgcgtgatcg actttagcgg taattacgaa gattatctgc
    85861 gtagtaaagg gatcgacggt taatttcccg ccgtctttca tgcggcagat gcattggctt
    85921 cacctgttca ccccggtcac ttacttattt aagctcccgg ggtttacagg tttgctgcct
    85981 tcctgcagcg tgaaatactg aggtaaagga ttcgatggct ggaccgggta aggcgcagtc
    86041 gcctcccggc cccggtaatg atttaacgca tccattcact cccttcaaca ccattgacca
    86101 gcagcgtgcg tttttcttcc actggtaatg acactttcag tgcactccat gcaggtcgat
    86161 aatccccgcg cgcgttgact ttcaggttga tggtcgcccc atcgcaaacc atttcccatt
    86221 ccacccaaag cgcattacca gtttggtagc cccagctttc gccatcgtct tcaaacagca
    86281 ggccggacgt tgtgcccaca cctttcagcg ggaacagttt cagttcgcgg gtatcgtctt
    86341 gttctgcgct cacataggtg atgcgtttgc tgagcggtag accagccccg gcacgcacca
    86401 tcagcggcag tttctccagc ggtgcgtcga gggtgatcca ctgaccgcca ctgaaccatt
    86461 cgccgttgta gaaatcgtac cagccagttt cgttatctgg cagccataca cggcgctggc
    86521 gctcgccttg ctctacgacg ctggcaacca aaatatcgcg accaagcagg aagtcatcac
    86581 actcttcaaa agtctgaacg tcgtgctcgt ggtcaaggaa agtcggacgt agcattggtt
    86641 catcatcggc gtatgcctgc cacatcaggg tataaaagta gggcagcagg cgatagcgca
    86701 actcaatagc gctgcggatc gccggagtga cgcccggata catccacggt tcgtttacgg
    86761 tatggtcatc gttccatgag tgaatcgtaa agcgcggatg catcacgccg ttttgtaccc
    86821 agcgtacaaa cagttccggg tccggcttat caccggagaa accgccgacg tcgtgaccga
    86881 ggttatacag cccggagagg ctcatcccca gacccatgcg ggtgttgtag cgcagagtgt
    86941 cccagctagt gcggttatcg ccactccagg tctgcacgta gcgctgcatt cccgcgcagc
    87001 cggagcggga gatcaagtac ggacgttttt ccggagcgaa acgctgctgg gcttccatcg
    87061 aggctcgcat catcagcagt ggcatcactg ggcgaatatg tttgatcgcg atcgtctgac
    87121 caaagccgtg acagcgtgct tcaccgtccc atacttcgaa ttcgttgttg tcgttccagg
    87181 tggagtcgat acccatctcc agaagctgtg tggtcacgcc ttcctgccac cactggatgg
    87241 tctgcggatt agtaaagtcg aggttagaac cttcgtcatc ccagaagctg gaacgttctg
    87301 gttcatctgt ttctgaatca cggatgaata gtcctctttc tgccacttcg ttatagcgtg
    87361 ggtgatcctg caacaggcac ggcttgatat tggcggccag gtgcaaacca gcattgtgaa
    87421 atgcctggct catcactttc ggctgcggca ctttttcgta gttccagttg aacacatagc
    87481 gtttgccgtt aattgaggta taaccggatg agagctggaa cgaatcgcag ggaattgcat
    87541 gttcttcaca caggcggata aagttcatca actgattttg tgcgtccggc gcatcggtgt
    87601 agtgcatagt tgaaccgctg tagcccaggc tccatttcgg tccgaacagg gttttgccgg
    87661 tcaggcgtac gaaggctttg gtgatatcaa gtacgcgttc gccggtaaac aggtagtagt
    87721 cgatatcacc cgcttccgcc tgccagcggc gataggcggt gtggtagttg tcgatctcgt
    87781 tgcccagatc cagccagcag ctgctgaggt tgtcataaaa caggccgaag ctaatgtcat
    87841 cgcggcgggt gatggtgaat ggaatgtgtt tgtacaacgg gtcagtactc acggcgttgt
    87901 agcccatcgc gtcgaggtta cgcatctcgt aacgtttacc gttgcgttgc aggtcgccgg
    87961 ctttatctcc gaggccataa aagcgttcat ctttacgacg gctctgatag tgcgccacgc
    88021 cgtcgccgtg ggcattcagc agataagcgc tggtcgggcg gtcattcacc aacggcagcc
    88081 attcgccttc gttgttacga tagtgccact ccagccacag cggctggtga acggtggcac
    88141 gcagtgactc ggtggcgata accacggtgt cgtcgtgctg gatcaatgtc cacgcgggca
    88201 ggctaaagcc gctgagatct tcgcggctgc gcccttccca tggcacatcc tgcgccggag
    88261 caatgctcca ggtgcgatcc agcgccagct cgcctttgcg ttttaacagc acgcggaaca
    88321 gattttcttc cagcacgtac aggcacagga tatgctggcc atcgaccagc agttcaacgt
    88381 ggtgggcaga ctgtttgtgc agggtccagt ttttcagcgt tttcatgttt acatccattc
    88441 tcaggcgcgt tttgcgcgac gttcagcaat aaaggtgacc aggaagaaag caccaatcag
    88501 gtcaaagaag cccatggcga taaacagggg gttaaagccg attttgtcag cggtaacccc
    88561 gataatcagg gagaacagga aacttgcgat ccacgcggca gaaccgcgca tgccgttaac
    88621 agtggccatc tgacctttgt cgaaggattc caccaccagt gcgctcagca tgcaggagat
    88681 gatctggtga ccgaagccgc cgatggaaat aaggacgatg gcgatatacg ggttattggt
    88741 aaaggccatc accgccagcg aaaccatcag gaatgcgcca ctgaccgatg atgcgacaac
    88801 ggagttcaca cgactcagcc caaacaggcg ggtgtaaagt ttggtcagat agccgctggc
    88861 aatactgcca agatcagcgg cgaggaacgg caaccaggcg aataatgcga tctgcttaag
    88921 gtccatgcca taggttttgg acaggtatag cggaacccag aaactcatca ccgcccacgc
    88981 cggttctgcc ataaaggccg ggatagcgat accgtaaaag cgtttatttt tgcctacggt
    89041 tttcagtgcg gtcagaaagg gtaacttgac agggggcgct tcgttatcct ggcggataaa
    89101 gtccagctcg gctttgctga ggttaggatg cttatccggg ttgtgataga acgcccacca
    89161 cagcaccacc cacagcatcg ccaggatgcc agtgaacata aatgcaccct gccagccgaa
    89221 tgaagcgtgg gcaaaataga taatcggtgg ggccagcatg gcaccgatgg aaaaaccgac
    89281 gcctgcccag cctgcggcga tcgggcgttc tgattttgga aaccattcac cgatggtttt
    89341 ggcgtttgct ggggttgccg cggcttccgc accgcccata aagaagcgca acagcgccag
    89401 atgtacccag tttcccgccc ccgcatgcat cagacaggcc agcgcccaca gtcccgcgca
    89461 gatcataaag cccagtttga ggccaatgac gtcaatcagc catccacaca acggttggaa
    89521 tagggtatag gcgagctgga aagcacctac aatccaggaa tattgttcgg tggtaatccc
    89581 caggttgctt ttcagttcgg gggcgagaat acccagcgag ttacgcgtga tgtagttgac
    89641 agtaacaccg agcaaaaaaa ggaccagtat ccaccaacgc agatttttga cgatatggcg
    89701 cggtttactg gcagtcatat cgttattgat accctgactc atatgcactc cacagaatgg
    89761 tatagggttt gtgtggcaac gtcagaatgg agttgtcaca ccacttttaa ctttttgata
    89821 aataaagtct ttattgttcg tttttccatt atttttgtat ggaagttgtt ctaccaattc
    89881 acagtaagag accctaaaag cgaaagaaag cgggttttgt gcacgttttt ccatgagtga
    89941 ctgatataca ttactgaaat ggatttaatg ccagacagaa tgggcttttt tgctcatgaa
    90001 aattctgtca tttccgaatt agaggcagat cacaaaatgg acaagaagct gaaaatcgcc
    90061 gatatcgccg cacgtaccgg gctatcgccg agtacggttt cgcgggtgct ggctggcaaa
    90121 gcgaatacca gtgcacgcgc tcgcgaacag gtgctggcct gcgcgcgtga gttgggcgtg
    90181 atggatggca tggcagcagg tcgtatgttg ctgaacaacc tgattatttt cgcgcagcaa
    90241 cgcgcttttg accaacgatc tgatatcttt tattaccgtg tgatccagag catcagcaaa
    90301 gcgttatcgc cgcatgaagt gcggctgcgt tactgcgcgc tggaggagtt tgacagcgat
    90361 cccgcgctat ttctctcgcg gatgaatgaa gcagatacgc aggcggcgat cctgctgggg
    90421 attgacgatc cgcacatcca tgatctggcc gccgatctcg ctaagccttg tgtgctgatc
    90481 aactgccgtg atgaacgaat gcgcctcccg gcgattgcgc cggaccatcg gttaattggc
    90541 gcgttcgccg cccgctatct gtttgaaatg ggtcaccggg aggtgatgaa tgtgatgtgc
    90601 ctgcgccgct acactatgga gcagcggttt gcagggatta aagaatcatg gcgtcagcat
    90661 aatctggcct ttcaccatga ccgtcatttg ctgaatatca gcagttttag cgctaaagaa
    90721 gcggaagaaa aggtcggagc gtggctggat gccgccaatg gcaagacgtt gccgacggcg
    90781 tttctggtcg gcggggattt tatggcggcg ggaacggtaa atgcgctaca tcgtcgtggt
    90841 ctgcgcgtac cacaggatgt ctcggtgatg agtatcgacg gctttaacct tgcggcaatc
    90901 gaggatgttc ccctgacggc tgtgcatgtg ccacgcgatg agctaggcaa tgaagcggtg
    90961 caaatgttgc aacagcggct gattcgcccg gatgctccgg tgggctcgct gctgctgcac
    91021 ggacgattgg cggtgcggga gtcagtgcgg cgggttcgcc caggaaaagg gaacaccgcc
    91081 gtggaacggg aagggctgta cgacactcag ctgtcgattc ccaacgctcg cttaccgtga
    91141 atgttgaggt cggcaagcgt aaagcgtcct tcccagtttg tttcatagtc ctcgcgcggg
    91201 aagtcgcccg gcgatgcgcc tttttccagt gcggccttca ccttgtgcgc gtaggcaaga
    91261 tttttctcgc acatcggtgc cgcaggaatg tacatcacgt tgccccaacc ctgttgattt
    91321 tcaacggggg caacggaatg gatgacgtcg cagtgccacc agacggagtc tccggcctcg
    91381 agttttggaa tgctggttaa cgcctcaatt aacagtggat gccattgctc tgataccggc
    91441 aacactcttc cgggcgctac gccgcacagt tcatcctccg gcacatcatc aagcagcgga
    91501 cgtaacagta cgtacgccat ggcttcagga atgggcacga cgtgcagcaa cccctgacca
    91561 ggcagcatat cagagagcgc tgtccagccc tggaatgtcc gaaacacgga acatttggtg
    91621 gtgttgtcca ccgtgtactc ttcaacttcc gtacgatgtg ccgcatgcca gggatcgtat
    91681 ttcgccagat tgccattaaa gacgttggcg aaaacgtgct gatacgctgg aagcagccag
    91741 cgttccagcg ccccggagtc ggtatgcgct ccaagacctt tggaggtcgt tccgggcgga
    91801 cggcggcgga tacggtcagg gtagatgacg ctcacatccg ggttaaacca ttgctttcca
    91861 tcactttcaa atgtccacag acgattgaga aacgactgca cattcgccat ttcttcactc
    91921 tggcgggcct gcatttgcgc ctgcgaccag tagatggggt aaatctcggg acgtgaagcg
    91981 ctgagcgtcc cgaagaaatt atcgccgggg cctttgtaga cctcgtcaaa gcggttgcgg
    92041 tccagatagt ccagcatcga ctgatcccag cctagcgctt gttcgcgggg gaaatggcct
    92101 tttatcaccg cacaaccgcg acgtttaatc tgttcgcgct gctctgcagt aacatgacct
    92161 gctttgatat cggcataaga cagcacc
//
LOCUS       DAYPHN010000002        83358 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000002 DAYPHN010000000
VERSION     DAYPHN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.002"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..551
                     /locus_tag="ACG0BW_000098"
     CDS             <1..551
                     /locus_tag="ACG0BW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFK7257118.1"
                     /translation="YNDIQTYDEQVNASWSEVLNQYQRRADLIPNLVASIKGYSSHER
                     DVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISERYPELK
                     SQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTPKKNYL
                     PDDVAAVSKAPTIDFSQNANAH"
     gene            565..1236
                     /locus_tag="ACG0BW_000099"
                     /pseudo
     CDS             565..1236
                     /locus_tag="ACG0BW_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1473..2621
                     /locus_tag="ACG0BW_000100"
     CDS             1473..2621
                     /locus_tag="ACG0BW_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFK7257119.1"
                     /translation="MRIFLLLLMLCGFQVFAEQLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQASQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPGRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            2618..3532
                     /locus_tag="ACG0BW_000101"
     CDS             2618..3532
                     /locus_tag="ACG0BW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFK7257120.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYAARVFDSWKLGDKQRNDGILLLVA
                     WEEHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELSEDRGDYYSINPFIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLLIFWLIVNKKLRAKYKKLGKERASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3592..4890)
                     /gene="eno"
                     /locus_tag="ACG0BW_000102"
     CDS             complement(3592..4890)
                     /gene="eno"
                     /locus_tag="ACG0BW_000102"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFK7257121.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4977..6614)
                     /gene="pyrG"
                     /locus_tag="ACG0BW_000103"
     CDS             complement(4977..6614)
                     /gene="pyrG"
                     /locus_tag="ACG0BW_000103"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFK7257122.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6842..7633)
                     /gene="mazG"
                     /locus_tag="ACG0BW_000104"
     CDS             complement(6842..7633)
                     /gene="mazG"
                     /locus_tag="ACG0BW_000104"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFK7257123.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7729..9963)
                     /gene="relA"
                     /locus_tag="ACG0BW_000105"
     CDS             complement(7729..9963)
                     /gene="relA"
                     /locus_tag="ACG0BW_000105"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFK7257124.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10011..11312)
                     /gene="rlmD"
                     /locus_tag="ACG0BW_000106"
     CDS             complement(10011..11312)
                     /gene="rlmD"
                     /locus_tag="ACG0BW_000106"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFK7257125.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASEDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLH
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11369..14125
                     /gene="barA"
                     /locus_tag="ACG0BW_000107"
     CDS             11369..14125
                     /gene="barA"
                     /locus_tag="ACG0BW_000107"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFK7257126.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14356..15696)
                     /gene="gudD"
                     /locus_tag="ACG0BW_000108"
     CDS             complement(14356..15696)
                     /gene="gudD"
                     /locus_tag="ACG0BW_000108"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFK7257127.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(15717..17057)
                     /gene="gudX"
                     /locus_tag="ACG0BW_000109"
     CDS             complement(15717..17057)
                     /gene="gudX"
                     /locus_tag="ACG0BW_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFK7257128.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17059..18411)
                     /gene="gudP"
                     /locus_tag="ACG0BW_000110"
     CDS             complement(17059..18411)
                     /gene="gudP"
                     /locus_tag="ACG0BW_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFK7257129.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(18886..19335)
                     /gene="yqcA"
                     /locus_tag="ACG0BW_000111"
     CDS             complement(18886..19335)
                     /gene="yqcA"
                     /locus_tag="ACG0BW_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFK7257130.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            complement(19353..20135)
                     /gene="truC"
                     /locus_tag="ACG0BW_000112"
     CDS             complement(19353..20135)
                     /gene="truC"
                     /locus_tag="ACG0BW_000112"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFK7257131.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20135..20464)
                     /gene="yqcC"
                     /locus_tag="ACG0BW_000113"
     CDS             complement(20135..20464)
                     /gene="yqcC"
                     /locus_tag="ACG0BW_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFK7257132.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLENNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21086..21631)
                     /gene="syd"
                     /locus_tag="ACG0BW_000114"
     CDS             complement(21086..21631)
                     /gene="syd"
                     /locus_tag="ACG0BW_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFK7257133.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRAHLASNLAEFLNQLKPLL"
     gene            21699..22547
                     /gene="queF"
                     /locus_tag="ACG0BW_000115"
     CDS             21699..22547
                     /gene="queF"
                     /locus_tag="ACG0BW_000115"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFK7257134.1"
                     /translation="MSSYANHQVLAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22659..24023
                     /gene="ppnN"
                     /locus_tag="ACG0BW_000116"
     CDS             22659..24023
                     /gene="ppnN"
                     /locus_tag="ACG0BW_000116"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFK7257135.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            24580..25869
                     /gene="sdaC"
                     /locus_tag="ACG0BW_000117"
     CDS             24580..25869
                     /gene="sdaC"
                     /locus_tag="ACG0BW_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFK7257136.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            25927..27294
                     /gene="sdaB"
                     /locus_tag="ACG0BW_000118"
     CDS             25927..27294
                     /gene="sdaB"
                     /locus_tag="ACG0BW_000118"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFK7257137.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            27405..28160
                     /gene="xni"
                     /locus_tag="ACG0BW_000119"
     CDS             27405..28160
                     /gene="xni"
                     /locus_tag="ACG0BW_000119"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFK7257138.1"
                     /translation="MAVHLLIVDALNLIRRVHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRTAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(28265..29413)
                     /gene="fucO"
                     /locus_tag="ACG0BW_000120"
     CDS             complement(28265..29413)
                     /gene="fucO"
                     /locus_tag="ACG0BW_000120"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFK7257139.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(29441..30088)
                     /gene="fucA"
                     /locus_tag="ACG0BW_000121"
     CDS             complement(29441..30088)
                     /gene="fucA"
                     /locus_tag="ACG0BW_000121"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFK7257140.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            30635..31951
                     /gene="fucP"
                     /locus_tag="ACG0BW_000122"
     CDS             30635..31951
                     /gene="fucP"
                     /locus_tag="ACG0BW_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFK7257141.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            31984..33759
                     /gene="fucI"
                     /locus_tag="ACG0BW_000123"
     CDS             31984..33759
                     /gene="fucI"
                     /locus_tag="ACG0BW_000123"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFK7257142.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLTDIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            33868..35286
                     /gene="fucK"
                     /locus_tag="ACG0BW_000124"
     CDS             33868..35286
                     /gene="fucK"
                     /locus_tag="ACG0BW_000124"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFK7257143.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            35288..35710
                     /gene="fucU"
                     /locus_tag="ACG0BW_000125"
     CDS             35288..35710
                     /gene="fucU"
                     /locus_tag="ACG0BW_000125"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFK7257144.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            35768..36499
                     /gene="fucR"
                     /locus_tag="ACG0BW_000126"
     CDS             35768..36499
                     /gene="fucR"
                     /locus_tag="ACG0BW_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFK7257145.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(36543..37643)
                     /gene="rlmM"
                     /locus_tag="ACG0BW_000127"
     CDS             complement(36543..37643)
                     /gene="rlmM"
                     /locus_tag="ACG0BW_000127"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFK7257146.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(37636..38031)
                     /gene="ygdD"
                     /locus_tag="ACG0BW_000128"
     CDS             complement(37636..38031)
                     /gene="ygdD"
                     /locus_tag="ACG0BW_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFK7257147.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(38050..38967)
                     /gene="gcvA"
                     /locus_tag="ACG0BW_000129"
     CDS             complement(38050..38967)
                     /gene="gcvA"
                     /locus_tag="ACG0BW_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFK7257148.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(39319..39546)
                     /gene="ygdI"
                     /locus_tag="ACG0BW_000130"
     CDS             complement(39319..39546)
                     /gene="ygdI"
                     /locus_tag="ACG0BW_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFK7257149.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            39738..40943
                     /gene="csdA"
                     /locus_tag="ACG0BW_000131"
     CDS             39738..40943
                     /gene="csdA"
                     /locus_tag="ACG0BW_000131"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFK7257150.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQCLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIASVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            40943..41386
                     /gene="csdE"
                     /locus_tag="ACG0BW_000132"
     CDS             40943..41386
                     /gene="csdE"
                     /locus_tag="ACG0BW_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFK7257151.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAVAKQV"
     gene            complement(41437..42243)
                     /gene="tcdA"
                     /locus_tag="ACG0BW_000133"
     CDS             complement(41437..42243)
                     /gene="tcdA"
                     /locus_tag="ACG0BW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFK7257152.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(42482..43579)
                     /gene="mltA"
                     /locus_tag="ACG0BW_000134"
     CDS             complement(42482..43579)
                     /gene="mltA"
                     /locus_tag="ACG0BW_000134"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFK7257153.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            43788..43864
                     /locus_tag="ACG0BW_000135"
     tRNA            43788..43864
                     /locus_tag="ACG0BW_000135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43822..43824,aa:Met,seq:cat)
     gene            complement(43939..45192)
                     /gene="amiC"
                     /locus_tag="ACG0BW_000136"
     CDS             complement(43939..45192)
                     /gene="amiC"
                     /locus_tag="ACG0BW_000136"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFK7257154.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            45424..46755
                     /gene="argA"
                     /locus_tag="ACG0BW_000137"
     CDS             45424..46755
                     /gene="argA"
                     /locus_tag="ACG0BW_000137"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFK7257155.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(46817..48643)
                     /gene="recD"
                     /locus_tag="ACG0BW_000138"
     CDS             complement(46817..48643)
                     /gene="recD"
                     /locus_tag="ACG0BW_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFK7257156.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFSD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(48643..52185)
                     /gene="recB"
                     /locus_tag="ACG0BW_000139"
     CDS             complement(48643..52185)
                     /gene="recB"
                     /locus_tag="ACG0BW_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFK7257157.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(52178..55066)
                     /gene="ptrA"
                     /locus_tag="ACG0BW_000140"
     CDS             complement(52178..55066)
                     /gene="ptrA"
                     /locus_tag="ACG0BW_000140"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFK7257158.1"
                     /translation="MPSSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVLNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(55242..58610)
                     /gene="recC"
                     /locus_tag="ACG0BW_000141"
     CDS             complement(55242..58610)
                     /gene="recC"
                     /locus_tag="ACG0BW_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFK7257159.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEAGNLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLEGSQELDAFVDVTPDNL
                     LHNIQSDILELENCAVAGVNIEEFSRSDNKRLLDPLDNSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPHGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(58623..58946)
                     /gene="ppdC"
                     /locus_tag="ACG0BW_000142"
     CDS             complement(58623..58946)
                     /gene="ppdC"
                     /locus_tag="ACG0BW_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFK7257160.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(58931..59338)
                     /gene="ygdB"
                     /locus_tag="ACG0BW_000143"
     CDS             complement(58931..59338)
                     /gene="ygdB"
                     /locus_tag="ACG0BW_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFK7257161.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(59335..59898)
                     /gene="ppdB"
                     /locus_tag="ACG0BW_000144"
     CDS             complement(59335..59898)
                     /gene="ppdB"
                     /locus_tag="ACG0BW_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFK7257162.1"
                     /translation="MPVKELGFSLLEVLIAMAISSVLLLGAARFLPALQRDSLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVTRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(59889..60359)
                     /gene="ppdA"
                     /locus_tag="ACG0BW_000145"
     CDS             complement(59889..60359)
                     /gene="ppdA"
                     /locus_tag="ACG0BW_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFK7257163.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGALWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(60544..61338)
                     /gene="thyA"
                     /locus_tag="ACG0BW_000146"
     CDS             complement(60544..61338)
                     /gene="thyA"
                     /locus_tag="ACG0BW_000146"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFK7257164.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(61345..62220)
                     /gene="lgt"
                     /locus_tag="ACG0BW_000147"
     CDS             complement(61345..62220)
                     /gene="lgt"
                     /locus_tag="ACG0BW_000147"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFK7257165.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(62371..64617)
                     /gene="ptsP"
                     /locus_tag="ACG0BW_000148"
     CDS             complement(62371..64617)
                     /gene="ptsP"
                     /locus_tag="ACG0BW_000148"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFK7257166.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEG
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(64630..65160)
                     /gene="rppH"
                     /locus_tag="ACG0BW_000149"
     CDS             complement(64630..65160)
                     /gene="rppH"
                     /locus_tag="ACG0BW_000149"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFK7257167.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(65513..65659)
                     /gene="ygdT"
                     /locus_tag="ACG0BW_000150"
     CDS             complement(65513..65659)
                     /gene="ygdT"
                     /locus_tag="ACG0BW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFK7257168.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YRIG"
     gene            65845..66534
                     /gene="mutH"
                     /locus_tag="ACG0BW_000151"
     CDS             65845..66534
                     /gene="mutH"
                     /locus_tag="ACG0BW_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFK7257169.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            66603..67316
                     /gene="ygdQ"
                     /locus_tag="ACG0BW_000152"
     CDS             66603..67316
                     /gene="ygdQ"
                     /locus_tag="ACG0BW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFK7257170.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            67454..67672
                     /gene="ygdR"
                     /locus_tag="ACG0BW_000153"
     CDS             67454..67672
                     /gene="ygdR"
                     /locus_tag="ACG0BW_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFK7257171.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            67780..68820
                     /gene="tas"
                     /locus_tag="ACG0BW_000154"
     CDS             67780..68820
                     /gene="tas"
                     /locus_tag="ACG0BW_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFK7257172.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(68852..70045)
                     /gene="lplT"
                     /locus_tag="ACG0BW_000155"
     CDS             complement(68852..70045)
                     /gene="lplT"
                     /locus_tag="ACG0BW_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFK7257173.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAIAALWI
                     WQRRH"
     gene            complement(70038..72197)
                     /gene="aas"
                     /locus_tag="ACG0BW_000156"
     CDS             complement(70038..72197)
                     /gene="aas"
                     /locus_tag="ACG0BW_000156"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFK7257174.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSIEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFSHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            72783..73814
                     /gene="galR"
                     /locus_tag="ACG0BW_000157"
     CDS             72783..73814
                     /gene="galR"
                     /locus_tag="ACG0BW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFK7257175.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(73821..75083)
                     /gene="lysA"
                     /locus_tag="ACG0BW_000158"
     CDS             complement(73821..75083)
                     /gene="lysA"
                     /locus_tag="ACG0BW_000158"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFK7257176.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            75205..76140
                     /gene="lysR"
                     /locus_tag="ACG0BW_000159"
     CDS             75205..76140
                     /gene="lysR"
                     /locus_tag="ACG0BW_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFK7257177.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAAKEILTPEDFHSENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(76127..76819)
                     /gene="ygeA"
                     /locus_tag="ACG0BW_000160"
     CDS             complement(76127..76819)
                     /gene="ygeA"
                     /locus_tag="ACG0BW_000160"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFK7257178.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(76948..78366)
                     /gene="araE"
                     /locus_tag="ACG0BW_000161"
     CDS             complement(76948..78366)
                     /gene="araE"
                     /locus_tag="ACG0BW_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFK7257179.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(78681..79442)
                     /gene="kduD"
                     /locus_tag="ACG0BW_000162"
     CDS             complement(78681..79442)
                     /gene="kduD"
                     /locus_tag="ACG0BW_000162"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFK7257180.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(79472..80308)
                     /gene="kduI"
                     /locus_tag="ACG0BW_000163"
     CDS             complement(79472..80308)
                     /gene="kduI"
                     /locus_tag="ACG0BW_000163"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFK7257181.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(80596..81777)
                     /gene="yqeF"
                     /locus_tag="ACG0BW_000164"
     CDS             complement(80596..81777)
                     /gene="yqeF"
                     /locus_tag="ACG0BW_000164"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFK7257182.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVS
                     EYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            82033..83262
                     /gene="yqeG"
                     /locus_tag="ACG0BW_000165"
     CDS             82033..83262
                     /gene="yqeG"
                     /locus_tag="ACG0BW_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFK7257183.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQIEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        20472 a        22289 c        20342 g        20255 t
ORIGIN      
        1 gatataacga tattcaaacc tatgatgagc aggttaacgc ctcctggtct gaggtattga
       61 atcaatatca acgtcgcgcc gatcttattc ctaatcttgt cgccagcatt aaaggttatt
      121 ccagccatga acgagatgta ctggaagccg taacattggc tcgtagccag gctaatcgcg
      181 ccagtagcga tcttcagcaa actccgggcg atgaacaaaa attacaagcc tggcaacagg
      241 ctcaagcgca ggtgactcgc actctcggtc agttaacgat aattagtgag cgttaccctg
      301 agttaaaatc tcaggaactc tatcaaaatt taatggtaca gcttgaagga agtgaaaatc
      361 ggattgccgt tgcgcgagga agatatatta aagccattga acaatataat gtcactatcc
      421 gcaaattccc cgctgtgtta accgcaaagg tcatggatta cacaccaaag aaaaattatt
      481 tgccggatga tgtcgccgca gtaagtaaag ccccaacaat agattttagc cagaatgcta
      541 atgctcatta agcggggaag ttctatgcga aattttatct tccttatggc tttcttctgt
      601 tcatctgtgt ttgccacaca aattccagta cctgaatcac ccaagtatgt gaatgactta
      661 accggcacat taacaaacag cgaagtaaac accctgacca accagattaa agccctgacg
      721 caaaaaaact acgcacagtt ggtcgtgtta gtggttgaaa caactggcga tgaaaccatc
      781 gagcagtacg caacgcgggt attcgatagc tggaaaccgg gtgataaaga ccacgatgat
      841 ggcgttctac tgttagtcgc ctggcaggat cataccgtgc gtattgaaat cggttacgga
      901 ctggaaggta ttattacgga tgcacagtcc gggaaaatta ttcgcaacag tattattccg
      961 gcatttaaaa aaggggacct ggctggtggt ttgcaaaagg gaatcagcga tattgaaagt
     1021 cgtctgacgg ggaataattt agcaacgata acaccgaccg atcacccttt gccttttagc
     1081 ggttggtggg ctttgttagt ctgggcgata gtactgacgt ttatttctgc cagaggatat
     1141 atcaaaacgc tgggagtaat ttgcttcgca gctattgtac tggcctttgt tttacctata
     1201 gcaggcttca gcggaagctg gggagtactc gcaacattac tctgcttcgc cacaccgttc
     1261 ctggctgtcg ccatcgcttt tacccccttc ggcaaaaagg ttcgggattc gatgcgtgat
     1321 gccaaccagc catcacgcca tacaagaagc aattcttcct cccactattc atcatcgtca
     1381 tcctcttcat tcaacaatga caatttcagc ggtggtggcg gttcatctgg tggcggtggc
     1441 gcttccgggc gctggtaaca aaaggaattg atatgcgtat ttttctctta cttctgatgc
     1501 tatgcggttt tcaggtattc gctgaacaat tgccttcagt aactgactta accgggacat
     1561 taacgacaga agaacaaact gctttaactc aacaattaca gactctcgag cagcaaaacc
     1621 acttccaggt ggccgtgttg gtcacgccga ccacaggggg caaaaacatt aaacttgcag
     1681 cctcgcagag atacggggta tatcactgga aacccgttgg agagaagcga tatggcgaag
     1741 gtatcttaat tctcgtcgta tggccggaag ggctggcgtc aatgaagatt ggacatggac
     1801 tggaacagat gttgccgccg gaacaagctt cgcaaattgt tcgttaccat atgcagccag
     1861 aattcgaaaa aaacaatctc tttgctggat taacgggcgg aattgaaagc atcgcacagt
     1921 ttacccatat caaagcgaac cttagccctt tggatgcttt agccaatcat ctgtttgcta
     1981 atccgcaacg ttcgctgcct tgtcttgcct ggacactgct gatgatcgtt gccatcgttg
     2041 tcctctggcg atttgccagc cgcccagggc gtggcatatg gatgatatcg atgattaccc
     2101 ctatggtctg gtttttctcc tttcaggacg atgtcatcat tcggcgggta tcggtcgttt
     2161 gttttagttt attgttagca acaacctgca agcagcgtct cgcggtagtt tttaatatgt
     2221 gccgcgtatt tcctatgatg ctgaccccaa aaaataaaaa cgctaaagtg aaaaaacaaa
     2281 agaagactcc gcaggttcgt gaatcaggac gaaattcatt gtttgtcgtg atgctgataa
     2341 ttattgtggg atggtgcctg gtgttcgcca gtaataaaga tattgcattc atctcaacaa
     2401 ttattgggtt aattgatcat tttatcggcc caataaccat tgctggcatc gttctgctga
     2461 tcattgtggc caaatttact ggcaatctta agctgggatc aggagaaaaa agcaacagga
     2521 aaacggggaa tcgtaatgct cattctcgct cttcgtcgag aaattctttc cgcggtggtg
     2581 gcggttcttc aggaggcggc ggttcatccg gccgttggtg atcgtaatgc gcttaatctt
     2641 tattctactt gctttgtgga gccttcccgg gcttgcccag caaattgctg tgccggagct
     2701 gcgccaacaa gtgactgata ttactggtac gttaagcacg tctgaacagc aatcgctaac
     2761 gcagcagctg caggatatta cacataaaac acgggcgcag gtcgctgtat tagtagtgcc
     2821 gtccaccgga gacgattcca ttgaacaata tgcagcacgc gtttttgata gctggaaact
     2881 gggagataag cagagaaacg atggtatttt acttctggtt gcctgggagg agcatgctgt
     2941 tcgtattgag gttggctacg ggctggaagg ggtggttacc gacctgcaag cggcgaagat
     3001 tatcagagat attttgatcc ctgccttcaa aagtgatgac ctgatgggtg gattaacact
     3061 ggcaagcgaa aatatcggcg cgcttctgtt gaatggtgaa ttatcggaag acagggggga
     3121 ttattacagt atcaatccct ttattccatt atcgcttgct gtaattatat tgctggcggt
     3181 actttcatat tttattgttt ttaccgatcc gtcgaattta ccctggatca ctctcactgg
     3241 cgccatttac gggatggtat tcctctatgt tgccgagcca ggaccatgga ccaatttaat
     3301 cgttgcctgc ggtatgctca cgccttttgc catcgtacca ctgcttattt tttggcttat
     3361 cgttaataaa aagctacgcg ctaaatacaa gaagctcggt aaagaaagag cttcaagaaa
     3421 aggttcttct tcatcctctt ccggtggcgg ctcaagtggc ggaggctcag gcggtggctt
     3481 tagcggcggg ggcggttctt ctggcggtgg cggcgcgtcc ggtcgctggt aactcaacca
     3541 taaaaaatgc cagcccgaag gctggcattt ttaaatcaga taaagtcaga cttatgcctg
     3601 gcctttgatc tctttacgac cgttgtacgg tgctttttcg cccagagctt cttcgatacg
     3661 aatcagctgg ttgtatttag caacacggtc agaacggctc atagaaccag ttttgatctg
     3721 gcctgcagca gtaccaacag ccaggtcagc gatggtagca tcttcagttt cgccagaacg
     3781 gtgagagatt actgcagtgt agccagcgtc tttcgccatc ttgattgcag ccagagtttc
     3841 ggtcagagaa ccgatctggt tgaatttgat caggatggag ttagcgatac ctttttcgat
     3901 accttctttc aggatcttgg tgttggttac gaacaggtcg tcaccaacca gctggatttt
     3961 gtcgcccaga actttggtct ggtatgcgaa accgtcccag tcagattcgt ccagaccgtc
     4021 ttcgatagaa acgatcgggt actgtttggt cagttcttcc aggaagtgag tgaattcttc
     4081 agaggtgaac gctttgttgc cttcgccagc cagaacgtat ttaccatctt tgtagaattc
     4141 agaagctgcg cagtccatcg ccagagtgat gtctttgccc agttcataac cagcagcttt
     4201 aacagcttca gcgataacag ccagagcttc agcgttggaa cccaggttcg gcgcatagcc
     4261 accttcgtca ccaacagcag tgttcatgcc tttcgctttc agaacttttg ccaggtgatg
     4321 gaaaacttca gaacccatgc gaatggcttc tttcacagtt ttcgcgccaa ccggctgaat
     4381 catgaattcc tggatatcaa cgttgttgtc agcgtgctca ccaccgttga tgatgttcat
     4441 catcggaacc ggcatggagt atttgcccgg agtgccgttc agttcagcga tatgctcata
     4501 cagcggcatg cctttagcgg cagcagcagc tttggcgtta gccagagata cagccaggat
     4561 tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc aggtcgatca tgatcttgtc
     4621 aatgccagcc tgatctttgg catctttacc aatcagtgcc tgagcgatcg ggccgtttac
     4681 cgcagcaaca gctttggtta cgcctttacc caggaaacgg gatttgtcgc catcgcgcag
     4741 ttccagagct tcacgggaac cagtagaagc acctgacgga gcagctgcca taccgacgaa
     4801 accaccctcc agatgtactt cggcttcaac agtcgggtta ccacgggagt cgatgatttc
     4861 acgaccgatg atttttacga ttttggacat taggttttcc tcaagtcact agttaaactg
     4921 aaactccaga caaacaacgc gtacccaggg tacgcgttgc cgttctaact tttttcttac
     4981 ttcgcctgac gcttctggaa ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga
     5041 ccatcacgtg gagtagaagt aaactccgga tggaactggc aagccacgaa ccacgggtga
     5101 ttcggaactt cgatgatctc gaccaactga tcatccccgg aacggcccgc aacgcgcaga
     5161 cctgcatctt caatctgttt caacagcatg ttgttgactt cgtaacggtg acgatgacgc
     5221 tcaacaattg tcggcgcatt gtacagctgg cgaaccaagc tatcgtcaac caactggcac
     5281 tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct tctcgctacg aacttcaacg
     5341 ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa ccgggtactt acagtctggc
     5401 acaaattccg tagagttggc gttctccatg ttggcaacat ggcgagcgta atcaattaac
     5461 gccacctgca tacccaggca aatgcccaga taaggaatat tgttctcacg cgcaaaacgc
     5521 gcggtcgtaa tcatgccttc tacgccacga tagccgaaac cgccaggtac gaggattgcg
     5581 tccagacctt taaggatttc aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg
     5641 atgttgacgc tgacacgatt cttcagccca ccgtgtttca gtgcttcgat cacggatttg
     5701 taagcgtccg gcagttcaat gtacttgccg accataccaa tggtgacttc acttaccgga
     5761 ttcgcttctt cgaagataac ctgttcccat tcggacagat tcgcttccgg gcagtttaag
     5821 ctgaatcgtt tacaaatata atcgtccagc ccctgcgatt tcaacaggcc cggaatttta
     5881 tagatggaat cgacgtcttt cagagaaata accgcttttt ccggaacatt acagaacaat
     5941 gcaatcttcg cacgttcgtt cgccggaacg gcgcgatctg aacgacaaat caggatgtca
     6001 ggctggatac cgatggagag cagctctttt acagagtgct gagtcggttt ggttttgact
     6061 tcaccagacg ctgccatgta cggcaccagc gtcaggtgca taaacagggt gtgctcacgg
     6121 ccaatttcaa cagccatctg gcgaatcgct tcgaggaacg gcagggattc gatatcacct
     6181 actgtaccgc cgatttcgac cagtaccacg tcatgacctt cgccgccttc cagcacgcgc
     6241 tctttgatag cgttagtgat gtgcggaata acctgtacgg ttgcgccgag gtagtcaccg
     6301 cggcgttctt tacgcagaac gtcagagtag atacgacccg tggtgaagtt gttgcggcgg
     6361 ctcattttgg tacgaatgaa acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg
     6421 tcttcggtaa cgaacacttc cccgtgttgg attgggctca tagtacctgg atcgacgttg
     6481 atgtacggat ccagtttcat gatggtcaca ttgaggccac gggcttcaag aatggctgcg
     6541 agggaggctg cggcaatacc tttacccaga gaggatacga ccccgccggt cacaaaaata
     6601 tagttcgttg tcatgctgaa cctgagaagt taggttgaaa agacgatgga ataaccagga
     6661 cgggaaagca gtatacccga acatgacctg tgccacaaac tttcattatc cctcctcttc
     6721 gccagcgcac tattgaaatc aggagtgaga aaatagcccc tttggggtaa atgtttttga
     6781 cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatc gcgcttgacc acgtaattcc
     6841 cttagagatc aatttcctgc cgttttacct gttgccagac ttcttccatt gtttcgaggt
     6901 caacacctgt catttccagt ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac
     6961 gctcgaattt ttcgttcgct ttttgcaatg cgatttccgc ttttgtccct aaatgacgag
     7021 ccagattgac cgtggcaaac agcaggtcgc ccatttcctc ctccagttta gcctggtcga
     7081 taacagcctg ccgtgcctcg tacatcacct cgtcgatctc ttcgtagact ttatcgacta
     7141 ccggaccaag cgtcgtccag tcaaaaccaa cgttggcgca acgtttctgg attttttgcg
     7201 cacgcattaa agccggtaaa ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct
     7261 gcgcgcgctc ttcggttttg atttgctccc aacgggcaag cacttcacta ctattttcgg
     7321 cagaactgtc agcaaaaaca tgcggatggc gacgttctaa tttatcgctg atcgcggcgc
     7381 atatatcatt aaagtcaaag cgcccttctt cctgagccat ttgtgcgtaa aacaccacct
     7441 ggaacagcag atcgcccagt tcaccgcgaa gatcgtcaaa atcttcacgg gcgatggcgt
     7501 ccagcacctc gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt
     7561 tatcccacgg gcagccgttt tccggatcgc gcaggcgctg cataatagtg agcaaacggt
     7621 cgatttgatt cattagaatg tcctgaaaat tgcgggtctg tcaggtgtaa taccttcgag
     7681 taaaagatat cgctggatac ggctgacgcc atatccagct atgtaagact aactcccgtg
     7741 caaccgacgc gcgtcgataa catccggcac ctggttgagt ttacccagca cgcggcccag
     7801 cacttgcagg ttgtaaatct caatggtcat gtcgatggtc gccagttgct gtttggtgtc
     7861 gctacggctg gcaacgccaa gtacgttcac cttctcgttg gcgagaatgg tcgtgatatc
     7921 acgtaacaac ccactacgat cattagccac cacgcggacc accagcgaat atccggcgga
     7981 gtagctctcg ccccataccg catcaacaat acgttctggc gcatgggagc gcagttccgc
     8041 cagttgttcg caatcggcgc ggtgtactga aataccgcgt ccctgggtaa tgaagccgac
     8101 aatctcatct ccaggaatcg gctggcagca gcgcgcgatg tggtgcatca ggttaccgac
     8161 accttcaacc accacacgac cgttatcttt actgcggttt tgcggcgtat agcttttttg
     8221 ctgaagttgc ttcagcgcgg cggcatcctg ctcttcggca ctcggcttat taaattgcga
     8281 ttgcaggaag ttcaccatct gattgagacg gatatccccg ccaccaatcg ccgccagcaa
     8341 ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct
     8401 gatccccaga tgttccagct cgtcgtcaag gatttgccgc ccagccagaa tgtttttgtc
     8461 acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta
     8521 accgaggttt gggtttaacc agtcacggct ggggttcggc tgtttctggg tgataatttc
     8581 aatctggtcg cccatctgca gctggtaggt gaacggcaca atgcgcccgc caatttttgc
     8641 cccgatgcag cggtgcccga catcactgtg gatgtggtaa gcgaagtcca gcggcgttga
     8701 tcccgcaggc aaatcaacga cgtcaccttt cggcgtaaag acgtacaccc ggtcgtcaaa
     8761 gacctggctg cgtacttcgt cgagcatttc gccggaatca gccatctctt cctgccacgc
     8821 aatcagttta cgcagccagg caattcggtc ttcatgtccc gaacgtgcgc cgccagctgc
     8881 cgcgccttct ttatatttcc agtgcgcagc aacccccaac tctgcatctt catgcatctg
     8941 tttggtgcgg atttggatct caacggtttt tccacccggc cccagaacca cggtatgaat
     9001 agactgataa ccgtttggtt tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg
     9061 gcgatagtga gtgtgcacta tccccagtgc ggcataacaa tcctgtaaac gctcggcgac
     9121 aatacgtacc gcacgcacat caaacagctc atcgaaggcg aggttctttt tctgcatttt
     9181 gcgccagatg ctgtagatgt gtttcggtcg accatacact tcagctttaa cgccttcagc
     9241 tttcatctca gcgcgcagat ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg
     9301 gcgttcatgc agcagttttg cgatgcgttt gtattcggtc gggtggagat aacggaagca
     9361 gtaatcttcc agctcccatt tcagttgtcc aatcccaaga cggttcgcca acggcgcgta
     9421 gatattggtg cactcttttg ccgccagtac gcgttcatct tccggcgcgt cttttacttc
     9481 gcgcagatga gcaatacgct ctgccagttt gatgaccacg cagcgaaaat catcgaccat
     9541 cgccaataac atccggcgaa cgttatcgac ctgctcggag gaaacagaat cggtatgcgt
     9601 cgctttcagc tggcggatcg ccgccatatc acgcacgccg tgaataaggt taacgaccga
     9661 cttaccgacg ctctcacgca gcacatcttc gctgactacg ttggcatccg ccagagggaa
     9721 cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat gtcgagagga tctccaccat
     9781 ctcaacacca cgccacaata acagactggc atccggatgc ccctgcgtct gttgcagaca
     9841 atacgcccag gtttcggcta agcactcaca cgacttctgg ctggtaatac ccagacttgc
     9901 gatccatttt tccggatcaa attcaccagc cttattgata tgtgcacttc ttaccgcaac
     9961 catcgtcctc tcctttaggg accagacctg ccgaaatcgg caaatcgtaa ctatttaacg
    10021 cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc
    10081 agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc tatcccgagc cagcgttgca
    10141 gggttacagg atacataaac tatacgaata ggttccagtt ttataatttg ctgcataact
    10201 cctgcggcac ctgctcgcgc cgggtccagc aacactttat cgaagccgtt tttcgcccac
    10261 ggctgctttg tgacatcttc ttcaagattt tcgtgataaa acgtcacatt gtgtaagcca
    10321 ttaagacgcg cattctgctg gcctttttcc accagcgccg gaacaccttc tacacccacc
    10381 acactggcag cttgtgtcgc caatggcagt gtaaagttgc ccataccgca gaacagatcc
    10441 agtacgcgat cttcaggttg cacatccagc cattccaacg cacgcgctac cattttttgg
    10501 ttcacacccg cattgacctg aataaaatcg cgcgggctaa aagttaagcg caacccgttt
    10561 gagtcatacc agggcatctc accagagacg gtttcgagta tctcactatc gggggcgaga
    10621 tacagatcca ggccttcaga atgcgaaaag cgttccagtt tttcgcgatc tgccgaactt
    10681 agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg ttgcctgtac cagttcaaca
    10741 tgaccaagat ggcgcatagc ttgtaagctg cccaggcatg ccctgacttt gggcagcaat
    10801 gcttcaagtt ggggcactaa aatgggacat tgtttaacgt cgacaatgtc actggagccc
    10861 gctttgcgaa accccatctg aagttgctgt gttttcggta agtagttcaa acttaaacgc
    10921 gcgcgacggc gatagcccca gggaacatcg gcgatcactt cagagacctc gtgtttcatt
    10981 aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat cctcgctggc gtgttgttgc
    11041 tgacagccgc cgcatacgcc aaaatgaggg cagcgtggcg tttcgcgttc cgggctatcg
    11101 cttaaccggc gtacgacttt agcgcgggcg tattgttttt tatcttcagt aacagtaact
    11161 tccgcgtttt cctgtggcag taatccagga ataaataacg ctttgccgtt atgtcgtgcc
    11221 acgccctgac caaaagagtc gaggtcgttg actgaaacag ttatgatctg acgcgtcgtc
    11281 gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga ctttctcaat ttaacagtgt
    11341 gaccttaatt gtcccataac ggaactccat gaccaactac agcctgcgcg cacgcatgat
    11401 gattctgatc ctggcaccga ccgtccttat tggtttattg ctgagtatct ttttcgttgt
    11461 gcatcgctat aacgacttgc agcgtcaact ggaagatgcc ggagccagca ttattgagcc
    11521 gcttgcagtt tctactgaat atggcatgag cctgcaaaat cgcgaatcta tcggtcagtt
    11581 aataagcgta ctgcatcgtc gccattccga tattgttcgc gcgatttcgg tttatgatga
    11641 aaataaccga ctctttgtca cttccaattt tcatcttgat ccctcatcaa tgcagctcgg
    11701 cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt gacggcgata ttatgatcct
    11761 ccgcacgccg attatttctg agagttactc ccccgacgaa tcgcccagta gcgatgccaa
    11821 aaatagtcaa aatatgctgg gatatattgc gctggagctg gatcttaaat cggttcgctt
    11881 gcagcaatat aaagagatct ttatttccag cgtgatgatg ctgttttgta tcggtattgc
    11941 gcttattttt ggctggcgct taatgcgcga tgtaaccggt ccgattcgca acatggtgaa
    12001 taccgtcgac cgcatccgtc gcgggcaact cgacagccgg gtggaaggat ttatgcttgg
    12061 tgagctggat atgctgaaaa acggtatcaa ctcgatggca atgtcgctgg ctgcttatca
    12121 cgaagagatg cagcacaata tcgaccaggc gacgtccgat ctgcgtgaaa cgctggagca
    12181 gatggaaatt cagaacgttg agttggatct ggcgaaaaag cgcgcccagg aagcggcgcg
    12241 tattaaatcc gagtttctgg caaatatgtc acacgaactg cgtacaccac tgaatggtgt
    12301 tattggcttt acccgcctga cgctgaaaac agaattaaca ccaacgcagc gcgatcacct
    12361 gaatacgatt gaacgttcgg caaataattt gctggcaatt attaatgatg ttctcgactt
    12421 ctcgaaactg gaagcaggta agctgattct ggaaagtatt ccattcccac tacgcagcac
    12481 gctggatgaa gtcgttaccc tgctggcaca ttcttctcac gataaagggc tggaactgac
    12541 gctcaatatt aaaagcgacg tgcctgataa cgttatcggc gacccactac gattacagca
    12601 aatcatcact aacctggtag ggaatgcaat taaattcacc gagaatggca acattgatat
    12661 tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag attgaagtgc agattcggga
    12721 taccggcatt ggtattccgg aacgcgatca atcgcgctta ttccaggcct tccgacaggc
    12781 tgatgccagt atttcccgcc gtcatggtgg caccgggctg gggctggtga ttacacaaaa
    12841 actggttaat gaaatgggcg gcgatatttc gttccatagc cagccgaatc gcggttcaac
    12901 tttctggttc cacattaatc tcgatctgaa tccgaacatt attatcgaag ggccatccac
    12961 ccaatgcctc gccggcaagc gcctggccta tgtcgaacca aactccgcag cagcgcaatg
    13021 cacgctggat attttaagtg aaacgccgct ggaagtggtt tatagcccaa cgttctccgc
    13081 gctgcctccc gcgcattacg acatgatgtt attaggcatt gcggtgacct tccgcgagcc
    13141 gctaacaatg caacatgagc gattagcgaa agcggtatcg atgaccgatt ttctgatgct
    13201 ggcacttcct tgccatgcac aagtcaatgc tgaaaaactt aagcaagatg gtatcggcgc
    13261 gtgtctgctg aaaccattaa cacctacgcg cctgttacct gccctgacgg aattttgtca
    13321 tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat
    13381 ggcggttgat gacaaccccg ctaacctgaa acttatcggc gcattgctgg aagatatggt
    13441 gcaacatgtg gaactttgcg atagcgggca tcaggcggtt gaacgggcga aacagatgcc
    13501 gttcgatttg atcttaatgg atattcaaat gccggacatg gacggcattc gggcctgcga
    13561 actcatccac cagctccccc atcagcaaca aacgccagtt atcgcggtaa cggcgcatgc
    13621 aatggccggg caaaaagaga agctgcttgg cgcagggatg agcgattatc tggcgaaacc
    13681 gattgaagaa gaacgattgc ataatttgct gttgcgctac aagcctggca gcggtatttc
    13741 ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg aacccgaatg cgaccctcga
    13801 ctggcaactg gcactacgcc aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca
    13861 aatgttactc gatttcctgc ctgaagttcg caacaaagtt gaggaacagc tggttggaga
    13921 aaacccggaa ggcctggttg atttgattca taaactgcat ggcagttgcg gctatagcgg
    13981 tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa cagctacgta gcggtactaa
    14041 agaagaagat ttggagccgg agctgctgga actgttggac gagatggata atgtagcgcg
    14101 cgaagccagc aaaattctcg ggtaatggac gtcttgtcga gtagcaatcg gctttttatg
    14161 acgctggttt cagattggtg acaaggtgcc tgtgtttatg ccggatgcgg cgttaacgcc
    14221 ttatccggcc tacaaaacca attaaattca atgaattaaa aagtatgttg gcctgatacg
    14281 tagcgcaaca ggcaattttg cgtttggcat cagtctcaaa ccggctcctg aaagagccgg
    14341 ttttggtttt ctgtcttaac gcaccatgca cgggcgcttg ttatcaaacg tccagccagg
    14401 aatcaggtac tgcattccca tcgcatcgtc acgcgcacca agcccatgtt tctgatacag
    14461 ctcgtgggct ttcatcacct gatccatatc gatttctacg cccaatcctg gtttttgcgg
    14521 cacctgtacc agcccgcctt tgatctcaaa cggttctttg gtcagacgct gattgccttc
    14581 ctgccagatc cagtgcgtat caatagcagt aattttaccc ggtgcagcag cggcaacgtg
    14641 ggtaaacatc gccagggaaa tatcgaaatg attgttagag tgtgaacccc aggtcaggcc
    14701 aaattcatgg cacatttgcg ccacacgtac cgaaccttgc atcgtccaga aatgcggatc
    14761 cgccagcggg atatcaacgg attgcaggga gagcgtatgg cccatctgcc gccagtcggt
    14821 ggcgatcata ttggttgcag tcggcagacc tgtcgcgcga cggaactctg ccatcacttc
    14881 acgcccggag aaaccttgct ccgcaccaca cggatcttct gcataagcca gcgaaccttt
    14941 caggtattta ccaattttaa tcgcctcgtt cagcgaccag gcaccgttag gatcgagggt
    15001 aatacgcgcc tgtgggaagc gttgcgccag tgccacaata gattctgctt cttcttcccc
    15061 ggccagtaca ccgcccttca gtttgaaatc gttgaagcca tatttttcat atgccgcttc
    15121 cgccaggcgc accaccgcat ccggcgtcat cgcttcttcg tgacgcaggc gataccagtc
    15181 acatttatca tccggctggc tttgatacgg cagcggtgtg gctttgcgat taccgacgaa
    15241 gaacagataa ccgagcattt cgacttcgct acgctgttga ccatcgccca gcagcgatgc
    15301 cacgttaacc cccagatgct gccccagcag atccagcatt gccgcttcta tcccggtaac
    15361 cacatgaata gtggtccgca ggtcaaatgt ctgcaaaccg cgcccgccgg catcacgatc
    15421 ggcaaatgta ttacgcacca gcgtcagaac gtttttgtat tcacccagcg ttttacccac
    15481 caccagcgga atcgcatctt ccagcgtttt acggattttc tcgccgccgg gaatttcccc
    15541 tacgccagtg tgaccagaat tatctttgat aatcacaata ttacgcgtga agaacggtgc
    15601 gtgtgcacca ctcagattca tcagcatact gtcatggccc gccaccggga tgacctgcat
    15661 ttcagtaaca acaggcgtcg taaattgaga actcatattt atgtcctttt tcaaaatcaa
    15721 tgacggccga aaacgggacg tttacggtca aaggtccagc cggggatcag gtactgcatc
    15781 gggcccgcgt cgttacgcgc accgccaggc agacgtttat aggcctcatg cgccttttgt
    15841 acctgctccc agtccagttc cacgcccaga ccgggcgcat caggaacggc aatttttccg
    15901 tttttaatct ccagcggatt tttggtcagg cgacaatcgc cctcctgcca aatccagtgg
    15961 gtatcgatag cggtaggatt acccggtgcc gccgcaccca catgggtaaa catcgccaga
    16021 gagatatcaa agtggttatt ggaatggcag ccccaggtca gcccccagtc gtcgcaaagc
    16081 tgcgctacgc ggactgcacc ggaaagcgtc cagaaatgcg gatcggcaag tggaatatcc
    16141 accgcattaa gcattaccgc atggcccatt tcgcgccagt tggtggcaat catgttggtc
    16201 gcgacgggca ggccggtcgc ccgtcgaaac tcagccatca cttcacgacc ggagaaaccc
    16261 tgctcagcgc cgcatggatc ttcagcatag gtgagaacat cattcagccc tttgcacaga
    16321 gaaattgctt catcaagcag ccatgcaccg ttgggatcaa cggtaattcg cgcatccggg
    16381 aagcgtttct tcaatgcacg aacagtgtcg atttcttgct cgccaggtaa caccccgccc
    16441 ttaagtttga aatctttaaa gccatagcga tcctgtgaag cttccgccag acgcacaacg
    16501 gcttcgctgt tcatcgcttt ctgatgccgc aattgatacc actcatggtt gcccggcgta
    16561 ttttccagat aaggaagatc ggttttggtc cgatcaccga tataaaacag ataaccgagg
    16621 acggtaatag cctcgcgttg cttgcctggc cccaacagtt cgcagaccgg aacattcagc
    16681 gccttaccta gcaggtcaag caaggcggct tccagcgccg ccacggcatt aacgcgcaat
    16741 tcaaaagtcc aggcaccttt gccgaaggta tcaaaatcgg ctgcctgatt acctttatgc
    16801 acctgctgaa ccactttatt caggcgcgca acttcctggc ccagcaccat cggaatagca
    16861 tcgaccaatg tctgataaat cacctctccg cctggcgctt caccaatgcc ggtatgcccg
    16921 gcgttatcgg tgagtaccac aatattgcga gtgaaatatg cgttatgcgc cccaccaata
    16981 ttaagcaaca tgctatcgtg cccggcgacc ggaatgactt tcatatcagt aataacagga
    17041 ctggattgtg tcgtcatctt attgccccgc gacaggtttc agctcgatac gcttgatatc
    17101 gcccaccagc accaggtagc tcagtaccgc gattaaggca tgaacaccaa cataaatcag
    17161 cgccccatta aaggagccag tcgtgccaac gatataacca attgcgattg gcgtgacgat
    17221 gccagaaatg ttaccgaaca tgttgaacag gccaccggaa agaccgctga tctcttttgg
    17281 cgcggtatct gccattactg cccaacccag cgccccgatg cccttaccga agaaggccag
    17341 cgccataaag ccgatgatca tccactcaac gttgacgtag ttgcagaaca ccatcaccat
    17401 cgacagcaac atgcccatta cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg
    17461 gcgcatcagc cagtcggaaa taatcccacc cagcacaccg ccgataaaac cgcaaaccgc
    17521 cggaacggaa gccacaaagc ccgctttcag aatcgacatc ccacgcgcct gcaccagata
    17581 aaccgggaac caggtaataa agaagtaagt cagggcgttg atacagtact gaccgatata
    17641 aacgccgatc atcatccgtg acccgagcag ttgtttgatc tgcccccact tcacgctgaa
    17701 cggcacttta actttggtgt tttgctgatc catattgatc agcgcgccac ccgcggcgat
    17761 gtactccagc tctttctgat ttacccccgg atgttgattt ggctcgtgga tgactttcaa
    17821 ccagataaag ctgatgacaa tccccagacc gcccataaag aagaagacgt gtgaccagcc
    17881 cacttcatgc gtcagccagc ccataatcgg cgcgaagatc accgttgcga agtattgagc
    17941 ggagttaaaa atcgacaccg ccgttcccct ttcctgcgcc ggaaaccagg ccgcaacaat
    18001 gcgactgttg ccggggaaag atggcgcttc agcaagcccg accaggaagc gcagcgtaaa
    18061 cagggcaacg ataatgccga atccactaaa gatatcgacg aagccttgca gcaaggtaaa
    18121 catcgaccag ataaagatcg accagaagta gacgcgtttt gaaccaaaac gatccagcaa
    18181 ccaaccacca gggatctgcc cgataacata agcccatgag aaggcagaga acacatagcc
    18241 cattcccacg ggatcaaggc cgatatcttt ggccatttcc gaaccggcaa tagaaagcgt
    18301 agcgcggtcg ccgtagttga aggatgtgac gataaacaac atcaccacta tccagtaacg
    18361 agcattcgtg cgtttttcca cactgctcgc agcctgactt aaagaactca ttgttgcact
    18421 cctgaaaatt cgcgttagcc acgctcactc tggactgcga catcgccagg aaatcagagg
    18481 tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat
    18541 atctggcaat agcagtataa aaagcgcgcc atagcggctc accgtgcaac aacacaacat
    18601 taatgcgttc aatgaggccc aattttggca ttagccctgg acggtggaat ccacttcacg
    18661 gaaatgaaaa caagaacaag aaaggaaggg ttaaaacgaa gaaataaaga agagtatgaa
    18721 atggatcgct tgactccagg caaacgccag taaaaatccg cgctatgaag cagtttttac
    18781 tggcatttgc ctgaaaagat tcgattcagc accgctaaaa cggcatttac accgctaaaa
    18841 cggcatttac accgctaaaa cggcatttac cgctcgctga acgtatcagg acaacagcgt
    18901 gccccaatgt tcgacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    18961 aatcaacagc atttctccca ctcgctgagc gctctgttcc tgcaacaagg catcgaactg
    19021 tttgccgcca ttgcagaaat tcacataact actgtcgccg agcgcaatca cgccataacg
    19081 cagattcggc tggaagccca gggtatcttt gattccctga aagagcggca caatgctatc
    19141 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    19201 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gtgcggtcag
    19261 aattgcttct gcttcttccg ccactaacag cgaatttccg tacatggtgc cgacaaaaat
    19321 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    19381 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccgcgcca gccaaattgt
    19441 gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta
    19501 aaaggatgcg tcaatgacaa ctgactggca tgcaacatta accgctgaag gccaaaatgt
    19561 tcagcaccgc tgcgattctg gcgtaaatca ccatgtttgc tgtcgccaat aatcgggtgg
    19621 cgcaaatggg caagatggcg acggagctgg tgcttgcgtc cggttttcgg ctccagttcc
    19681 accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc
    19741 agaccgcgat aatgcgtcac tgctggctgt gggcctttat cttcgcgggc aaatttatca
    19801 gcgattttgt ccagttcttc caccagtgga taatccagaa ctgcttcttc catcaaccag
    19861 ccgcgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    19921 agccgtccgg cctcgctgga taatcccatc aacaacacac cagaagttgg tcggtccaga
    19981 cgatgagcag taaacacatg ctggcctatc tggtcacgca cggtttgcat gaccactact
    20041 ttctcgtcgc gatccagcca gctgcggtga accagccagc cggagggttt atttaccgca
    20101 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    20161 catccagttt ttccagctca gccagaataa gcgcgcgttg cgggtggtcc gtcgccagcg
    20221 ccatttcata atagggagca acggcaaaag cgcccggtaa cggctggttg ttttctaaca
    20281 aatcgtgcat tcgcgggatc agcacccact gcaaccactc cagtggttcc atggtgtcca
    20341 taaagaacgg ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct
    20401 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    20461 tcataaagca acctcaataa gaaaaactgc cgcgaagaat agcaggaata aaaaaaggga
    20521 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc
    20581 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    20641 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg
    20701 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    20761 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    20821 ccttcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    20881 tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    20941 ttcatatgaa agttaaggat taacaagcaa tatattgatt ttaaatgaaa ataacgattc
    21001 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    21061 ctcacaacca aatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    21121 caagatttga ggctaaatgc gcccgtttac gtgttcccag cgtctcttta cacacttcac
    21181 cggagagatt acacaccgag atcacctcta actcttcttc cagcgtcgcg ataaatagcg
    21241 tgggcggcaa ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    21301 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttaatatcg ccaaactgcg
    21361 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa
    21421 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    21481 agtatacggc gtcttcggtg gttgaaataa tgcacggtga aggaacgccg tacagttcct
    21541 cactcaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    21601 ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    21661 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    21721 aggtacttgc gggcctgact cttggaaaat caaccgatta ccgggatacc tatgacgcca
    21781 gcctactgca aggcgttccg cgtagtctga atcgcgatcc gctgggtctg aaagcggata
    21841 acctgccttt tcacggtacg gatatctgga cgctatatga actttcctgg ctgaatgcga
    21901 aaggtttgcc gcaggtcgcc gtcggtcatg ttgaacttga ttacaccagc gcaaatctga
    21961 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    22021 gggatgaggt gcgccagacg ctggagcgcg acttaagcac ttgcgctcag ggtaaggtta
    22081 gcgtggcgtt atatcgtctt gatgaactgg aaggccaacc gataggtcat ttcaatggca
    22141 cttgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    22201 agaatgccac cagtggagaa aaagtagtag aagagacgct cgtcagccac ctgctgaaat
    22261 caaattgcct gatcacccat caaccagatt ggggatcgat ccaaattcag tatcgtggac
    22321 gccaaattga cagagaaaaa ctgctgcgtt acctggtttc tttccgtcat cacaacgagt
    22381 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    22441 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    22501 gtaatagcga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    22561 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc
    22621 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    22681 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    22741 gcgacctcta tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    22801 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc
    22861 gtgaacgcgg cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc
    22921 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgtgacatt ctcttcgttt
    22981 acgggcaaat ccataacacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    23041 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtt ggtgaagcgc
    23101 caaatatggt ggtctgctgg ggcgggcact caattaacga aaacgagtat ttgtatgccc
    23161 gtcgcgtagg gaatcagctg ggcctgcgtg agctgaatat ctgcaccggc tgtggtccgg
    23221 gagcgatgga agcgccgatg aaaggcgctg cggtcggaca cgcacagcag cgttacaaag
    23281 acagtcgttt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    23341 tggtcaacga attgatcatc atgccggata tcgaaaaacg tctggaagcg tttgtccgta
    23401 tcgctcacgg tatcattatc ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt
    23461 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    23521 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgtg catacgttgg
    23581 gtgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    23641 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg cgacacaggc gatgcctaca
    23701 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    23761 agaatatggc taatctgaag ctttacccgg atcaacccgt tgaagtgctg gctgccgacc
    23821 tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca
    23881 ttgaagagtt tggtccttat aaaatcaacg gcgataaaga gattatgcgt cgtatggatg
    23941 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    24001 cttgctacga aatctgcacg taatctcccc tcccccggtg acgtttgttg ccgggttttc
    24061 tcgtttttgg tcacttactc atcaactcat ttcatttgtt atatgaatgt ttcttaccac
    24121 cctcacgcaa caaatatcat cacagttaat atgtcataca atttatattg caacgcaaac
    24181 gtttccctat tttcataaac cacattttat cgctatgaaa aagaaattat cgccatgatt
    24241 aactaaaagt attgattttt tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc
    24301 agatgtcgtt taatgggcaa atattgccct taaattctct tttacttttg atttacagag
    24361 taaagcgttg ggataatcta tcttccaagt agattattgt atttgggatc aagatcactg
    24421 atagatacat aacttgtgtg tatctttccg ccctcaaatt attacggcgg taaatgatta
    24481 agccatcgcc gatagacaga tttcattttt acggtcaggc accttcccgg gctgaactgg
    24541 ctaaaagctg aattatttgc attcctccag gagaaataga tggaaacgac tcaaaccagc
    24601 acgattgcgt cgaaagactc tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg
    24661 ggcctttacg gcacggcaat cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt
    24721 ggcggtatga tcccgctgat catcatggct atccttgcgt tcccgatgac attttttgct
    24781 caccgcggcc tgactcgctt cgtactgtct ggtaaaaacc caggcgaaga catcaccgag
    24841 gttgtagaag aacactttgg tattggcgca ggtaaactga ttaccctgct ctacttcttc
    24901 gctatctacc cgatcctgct ggtttatagc gtggcaatca ccaatacagt tgaaagcttc
    24961 atgtctcacc agctgggtat gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc
    25021 gtgggtatga tgaccatcgt tcgcttcggt gagcagatga tcgttaaagc gatgagtatt
    25081 ctggtattcc cgtttgttgg cgtactgatg ctgctggctc tgtacctgat cccgcagtgg
    25141 aacggcgcag cactggaaac gctgtctctg gacactgcat cggcaaccgg aaacggtctg
    25201 tggatgaccc tgtggctggc aattccagta atggtgttct cgttcaacca ctctccgatc
    25261 atctcttctt tcgctgttgc gaagcgtgaa gagtacggcg atatggcaga acagaaatgc
    25321 tcgaagatcc tggcattcgc acacatcatg atggtgctga ccgtaatgtt cttcgtattc
    25381 agctgcgtac tgagcctgac tccggcagac ctggctgcgg ctaaagagca gaacatctcg
    25441 attctgtctt acctggctaa ccactttaac gcaccggtta tcgcgtggat ggctccgatt
    25501 atcgcgatta tcgctatcac caaatccttc ctcggccact acctgggcgc acgtgaaggc
    25561 ttcaacggta tggtgattaa atctctgcgt ggtaaaggta agtctatcga aatcaacaag
    25621 ctgaaccgta tcactgcgct gttcatgctg gtaacgacct ggattgttgc caccctgaac
    25681 ccgagcatcc tgggtatgat tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc
    25741 ctgatgccga tgtacgcaat tcagaaagta ccggcaatgc gtaagtacag cggtcacatc
    25801 agcaacgtat tcgttgtcgt gatgggtctg attgcaatct ccgcaatctt ctactctctg
    25861 ttcagctaag tcctttcgcg ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta
    25921 tttcctatga ttagcgtatt cgatattttc aaaatcggca ttggcccttc cagttctcat
    25981 accgttggac caatgaaagc gggtaaacaa tttaccgacg atctgattgc ccgtaacctg
    26041 cttaaagacg tgacccgcgt ggtggttgac gtgtacggct cgctctctct gactggtaaa
    26101 ggccaccaca ctgatatcgc cattattatg ggcctggcgg gtaacctgcc ggataccgtg
    26161 gatatcgatt ccatccctgg ttttattcag gatgtgaata ctcatggtcg cctgatgctg
    26221 gcaaacggtc agcatgaagt ggagttcccg gttgatcagt gcatgaactt ccatgccgac
    26281 aacctttctc tgcatgaaaa cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt
    26341 tacagccaga cttactactc tatcggcggt ggctttatcg ttgatgaaga acattttggc
    26401 cagcaggata gcgcaccggt tgaagttcct tatccgtata gttcagcagc cgatctgcaa
    26461 aaacattgtc aggaaaccgg gctgtcactc tctggcctga tgatgaaaaa cgagctggcg
    26521 ctgcacagca aagaagagct ggaacagcac ctggcgaacg tatgggaagt catgcgcggc
    26581 ggtattgagc gcggtatttc taccgaaggc gtgttgcctg gcaaactgcg cgtaccacgc
    26641 cgtgctgcgg cactacgccg gatgctggtc agccaggata aaaccaccac tgacccgatg
    26701 gcggttgttg actggatcaa catgtttgca ctggcagtga acgaagagaa cgctgctggc
    26761 ggtcgcgtgg tgactgcgcc gactaacggt gcgtgcggga ttatcccggc tgtgctggcg
    26821 tactacgata agtttatccg cgaagtgaac gctaactcac tggctcgtta cctgctggta
    26881 gccagcgcca ttggttctct ttataagatg aacgcgtcga tttctggtgc cgaagtgggt
    26941 tgccagggtg aagttggcgt ggcgtgctct atggcggcag ctggtctggc agaactgtta
    27001 ggcgcaagcc cggcgcaggt gtgcatcgcg gcggaaatcg ccatggagca taacctcggt
    27061 ctgacgtgtg acccggttgc tggacaggtg caggtaccgt gcatcgagcg taacgccatt
    27121 gcggcagtaa aagcggtgaa cgccgcgcgt atggcgctgc gccgtaccag cgagccgcgc
    27181 gtctgcctcg ataaagttat cgagaccatg tacgaaacag gtaaagatat gaacgccaag
    27241 taccgcgaaa cctctcgcgg tggcctggca atgaagatcg ttgcctgcga ttaatctctc
    27301 ccggaggcct cgttttgcga ggcctcttcc cgttttctca accagccata gcctgttccg
    27361 gcatcgaatg ttacccttat cgcctgatct ttaagggggt tatcgtggct gttcatttgc
    27421 ttattgtcga tgcactgaat cttattcgtc gtgttcatgc cgttcagggt tcgccctgtg
    27481 tcgaaacctg ccagcacgcg ctcgatcagc tcattatgca cagccagcca acccacgcgg
    27541 tcgccgtttt tgatgatgaa aaccgcagta gcggctggcg tcatcagcgt ttaccagatt
    27601 acaaagcggg tcgaccgcca atgccggaag agttgcacga cgagatgcct gcactacgca
    27661 ccgcctttga gcaacgcggc gtcccgtgct ggtcagccag cggtaacgaa gccgatgact
    27721 tagccgccac gctggcggtc aaagtgacac aggccgggca tcaggcaacg attgtttcga
    27781 cagataaagg ctactgtcag ttactttcac cgacattacg tattcgtgat tacttccaga
    27841 aacgttggct ggatgcgcca tttatcgata aagaatttgg cgttcaaccg cagcagttgc
    27901 ccgattactg ggggcttgcg gggatcagca gttcaaaggt accgggtgtt gcggggatcg
    27961 gacccaaaag cgccacgcag ctgctggtcg agtttcagag tctggaaggg atatatgaga
    28021 atctggatgc ggttgccgaa aagtggcgca aaaaattaga aacccataaa gagatggcgt
    28081 ttctgtgccg cgatattgcc cgcttacaaa ccgatttgca tatcgacggc aatttacagc
    28141 aattgcggtt ggtacggtaa cggcgtgccg gatgcaaacg attatcaaca cattgaattt
    28201 aatgaactta tacgccggat aagacgcagc tagcgtcgca tccggcatta tcacatcagc
    28261 gcatttacca ggcggtatgg taaagctcta caatatcctc aagcgttgct tcgcgcgggt
    28321 tgccaccggt acaaacgtca tcaagcgccg cctgcgccag tgccggaatg tcttccttgc
    28381 gtacaccaac gtcacgcaaa tgtggcggaa taccgacatc acggttgaga gcaaacaccg
    28441 cttcaacagc ggcattacgc gcctcttcca ggctcatacc ttccactttc acgcccataa
    28501 cgcgcgcgat atcgcggtac ttctcaccgg taaagtcagc gttatagcgc atgacatgcg
    28561 gtaacaggat ggcgttcgca acaccgtgtg gagtgttata aaacgcgccc agtggatgcg
    28621 ccataccatg caccaaccct aacccaacat tcgagaagcc catacccgca acatactgcc
    28681 cgagcgccat ttcttctccg gcatccttat caccagcaac cgatcctcgc agcgccccag
    28741 caatgatttc aatcgcttta atgtgcagtg catcggttag cgcccacgcg ccacgggtaa
    28801 tatacccctc aatagcatga gtgagcgcat cgacacccgt cgcagctttc agcgctggag
    28861 gcataccatc catcatgtca gcgtcaataa acgccacctg cgggatatca tgcggatcaa
    28921 cgcaaacaaa cttgcgccgt ttttcttcgt cagtgatcac gtagttaatg gtcacttctg
    28981 ccgcagtgcc tgctgtggtg gggattgcca gaatcggtac actaggttta ttggtcgggg
    29041 aaagtccttc caggctacgc acatcggcaa actccgggtt gttgctgata atgccaatcg
    29101 ctttacaagt atcctgtgga gaaccaccac caatagcgat caggtaatcc gcgccgctat
    29161 tctggaatac accgagccct tctttgacga cagtaattgt tgggttgggc actacgccgt
    29221 cgtaaatcgc ccatgccagc cctgcagcat ccatcttatc ggtcactttc gccaccacgc
    29281 cgcattgcac cagcgtttta tcggtgacga tcagcgcctt ctgataacca cggcgtttca
    29341 cctcatcggt taaagcccca acagcacccc gaccaaacca tgccgtttcg ttcagaatca
    29401 ttctgttagc catcatcctt ctccttgttg ctttacgaaa ttactcttca atccgtaacc
    29461 cataggtttt gaatttctcc agcactacgg caatctcttc atcgctcagc actggcaccg
    29521 ggtccgtaat cgccagggtc gtcaggtaaa gttgcgccag cacttcaact tcatgcgcca
    29581 gccataacgc tttttccaga ttcacctcac aagcgataag cccatgatgt tgtaacaaag
    29641 ttgccttacg atttttgaga gccagcgcaa catgttcaga aagttcgcgt gttccaaagg
    29701 tcgcataagg cgcgcaagga atagaattac cgccagccgc cgcaatcatg tagtgaatag
    29761 cggggatcgg tcggttaaga atggaaactg ctgtgcaatg aacggcatga ttgtgaacaa
    29821 ccgcgttggc atccggtctg ctttgatagg ccgccatatg gaaacgccat tcgcttgagg
    29881 ggagctttcc ttcctcatgt ttaccgttgc catcaataaa gacaatatgc gactccgtca
    29941 gtttttcata tggaatacct gtaggcgtaa tcagcatccc atcctgataa cgtacactga
    30001 cgttacccgc tgtcccctgg ttcagtccca ggcgggtcat ttccaggcaa gtgtcaataa
    30061 tctgacgagc aagtttattt cgttccatta gctacctctc tctgattcaa aacagggcaa
    30121 taatgttgtt cctttcacac tattgaatta gccgtttaat tacccaccat cttcttcctg
    30181 attaaccaga aagaaattca caagcttata ttttgtgacc tggttcaact aatcatagta
    30241 aataactgca agttctcttt ttataaccct attaaaaatg accgctctta aaaatattta
    30301 tcaaaacggt catttttcca ttccccctcg ccagaaatgc ccgttttcgg cacaaacaat
    30361 taatacggtc atcgggtttg tgttttttat gatttatttt ctgaaacggg catgaaattt
    30421 cgattattaa agtgatggta gtcacataaa gtcacctccc agctaataag tgtgaccgcc
    30481 gtcatattac agagcgtttt ttatttgaaa atgaatccat gagttcattt ctgacaggca
    30541 aatattcact gatatgaagc ccgaactcgc tggttttgca cttttgaaaa cataaccgat
    30601 tacgtgctta agcttctgaa cctaagagga tgctatggga aacacatcaa tacaaacgca
    30661 gagttaccgt gcggtagata aagatgcagg gcaaagcaga agttacatta ttccattcgc
    30721 gctgctgtgc tcactgtttt ttctttgggc ggtagccaat aaccttaacg acattttatt
    30781 acctcaattc cagcaggctt ttacgctgac aaatttccag gctggcctga tccaatcggc
    30841 cttttacttt ggttatttca ttatcccaat ccctgctggg atattgatga aaaaactcag
    30901 ttataaagca gggattatta ccggattatt tttatatgcc ttgggcgctg cattattctg
    30961 gcccgctgca gaaataatga actacacctt atttttagtt ggcctattta ttattgcagc
    31021 cggattaggt tgtctggaaa ctgccgcaaa cccttttgtt acggtattag ggccggaaag
    31081 cagtggtcac ttccgcttaa atcttgcgca aacatttaac tcatttggcg caattatcgc
    31141 ggttgtcttt gggcaaagtc ttattttgtc taacgtgcca catcaatcgc aagacgttct
    31201 cgataaaatg tctccagagc aattgagtgc gtataaacac agcctggtat tatcggtaca
    31261 gacaccttat atgatcatcg tggctatcgt gttactggtc gccctgctga tcatgctgac
    31321 gaaattcccg gcattgcaga gtgataatca cagtgacgcc aaacaaggat cgttctccgc
    31381 atcgctttct cgtctggcgc gtattcgtca ctggcgctgg gcggtattag cgcaattctg
    31441 ctatgtcggc gcacaaacgg cctgctggag ctatttgatt cgctacgctg tagaagaaat
    31501 tccaggtatg actgcaggct ttgccgctaa ctatttaacc ggaaccatgg tgtgcttctt
    31561 tattggtcgt ttcaccggta cctggctcat cagtcgcttc gcaccacaca aagtcctggc
    31621 cgcctacgca ttaatcgcta tggcactgtg cctgatctca gccttcgctg gcggtcatgt
    31681 gggcttaata gccctgactt tatgcagtgc ctttatgtcg attcagtacc caacaatctt
    31741 ctcgctgggc attaaaaatc tcggccagga caccaaatac ggttcgtcct tcatcgttat
    31801 gaccattatt ggcggcggta ttgtcactcc ggtcatgggg tttgtcagtg acgcagcggg
    31861 caacatcccc actgccgaac tgatcccggc actctgcttc gcagtcatct ttatctttgc
    31921 ccgtttccgt tctcaaacgg caactaactg aacatatttt ccgaataaag tgaggaatca
    31981 gtaatgaaaa aaatcagctt accgaaaatt ggtatccgcc cggttattga cggtcgtcgc
    32041 atgggtgttc gtgagtcgct tgaagaacaa acaatgaata tggcgaaagc cacggccgca
    32101 ctgctgaccg agaaactgcg ccatgcctgc ggagctgccg tcgagtgtgt catttccgat
    32161 acctgtatcg cgggtatggc tgaagccgct gcttgcgaag aaaaattcag cagtcagaat
    32221 gtaggcctca ccattacggt aacgccttgc tggtgctatg gcagtgaaac catcgacatg
    32281 gatccaaccc gcccgaaagc catttggggc tttaacggca ctgaacgtcc cggcgctgtt
    32341 tacctggcag cggctctggc agctcacagc cagaaaggca tcccggcatt ctccatttac
    32401 ggtcatgacg ttcaggatgc cgatgacaca tcgattcctg ctgatgttga agaaaaactg
    32461 ctgcgctttg cccgcgccgg tttggccgtc gccagcatga aaggtaaaag ctatctgtcg
    32521 ctgggcggcg tttcgatggg tatcgccggt tccattgttg atcacaactt ctttgaatcc
    32581 tggctgggaa tgaaagtcca ggcggtggat atgaccgaac tgcgtcgccg tatcgatcag
    32641 aagatttacg acgaagccga actggaaatg gcgctggcct gggctgataa aaacttccgc
    32701 tatggcgaag atgaaaataa caaacagtat caacgcaatg ccgagcaaag ccgcgcagtt
    32761 ctgcgcgaaa gtttactgat ggcgatgtgt atccgcgaca tgatgcaagg caacagcaaa
    32821 ctgaccgata ttggtcgcgt ggaagaatca cttggctaca acgccatcgc tgcgggcttc
    32881 cagggacaac gtcactggac cgatcaatat cccaatggcg acaccgccga agcgatcctt
    32941 aacagttcat ttgactggaa tggcgtgcgc gaaccctttg tcgtggcgac cgaaaacgac
    33001 agtcttaacg gcgtggcaat gctaatgggc caccagctca ctggtaccgc tcaggtattt
    33061 gccgatgtgc gtacctactg gtcaccagaa gcaattgagc gtgtaacggg gcataaactg
    33121 gacggactgg cagaacacgg catcatccat ttgatcaact ccggttctgc tgcgctggac
    33181 ggttcctgta aacaacgcga cagcgaaggt aacccgacga tgaagccgca ctgggaaatc
    33241 tctcagcaag aggctgacgc ttgcctcgcc gctaccgaat ggtgcccggc gatccacgaa
    33301 tacttccgtg gcggcggtta ctcttctcgc ttccttaccg aaggcggcgt cccgttcact
    33361 atgactcgag tcaacatcat caaaggcctg ggaccggtac tgcaaatcgc ggaaggctgg
    33421 agcgtggaat tgccgaagga tgtgcatgac atcctcaaca aacgcaccaa ctcaacctgg
    33481 ccaaccacct ggtttgcacc gcgcctcacc ggtaaagggc cgtttacgga tgtgtactcg
    33541 gtaatggcga actggggtgc taaccacggg gttctgacca tcggccacgt tggcgcagac
    33601 tttatcactc ttgcctccat gctgcgtatc ccggtatgta tgcacaacgt tgaagagacc
    33661 aaagtgtatc gtccttctgc ctgggccgcg cacggcatgg atattgaagg ccaggattac
    33721 cgcgcttgcc agaactacgg cccgttgtac aagcgttaat acattactcc ctgatgtgat
    33781 gcccggtcgc tccggctacc gggcctgaac aagcaagagt ggttagccgg ataaacaatg
    33841 ttatccggct atattgcagg agcgattatg aaacaagaag ttatcctggt actcgactgt
    33901 ggcgcgacca atgtcagggc catcgcggtt aatcggcagg gaaaaattgt tgcccgcgcc
    33961 tcaacgccta atgccagcga tatcgcgatg gaaaacaaca cctggcacca gtggtcttta
    34021 gacgccattt tgcaacgctt tgctgattgc tgtcggcaaa tcaatagtga actgactgat
    34081 tgccacatcc gcggtatcgc cgtcaccacc tttggtgtgg atggcgctct ggtagataag
    34141 caaggcaatc tgctctatcc gatcattagc tggaaatgtc cgcgaacagc agcggtaatg
    34201 gacaatattg aacggttgat ctccgcacag cagttgcagg ctatttctgg cgtcggagcc
    34261 tttagtttca atacgttata taagttggtg tggttgaaag aaaatcatcc acaactgctg
    34321 gaacgcgcgc acgcctggct ctttatttcg tcgctgatta accaccgttt aaccggcgaa
    34381 ttcactactg atatcacgat ggccggaacc agccagatgc tggatatcca gcaacgcgat
    34441 ttcagtccgc aaattttaca agccaccggt attccacgcc gactcttccc tcgtctggtg
    34501 gaagcgggtg aacagattgg tacgttacag aacagcgccg cagcaatgct cggcttaccc
    34561 gttggcatac cggtgatttc cgcaggtcac gatacccagt tcgccctttt tggcgctggt
    34621 gccgaccaaa atgaacccgt gctctcttcc ggtacatggg aaattttaat ggtccgtagc
    34681 gcccaggttg atacttcgct gttaagtcag tatgccggtt ccacctgcga actggatagt
    34741 caggcagggt tgtataaccc aggtatgcaa tggctggcat ccggcgtgct ggaatgggtg
    34801 agaaaactgt tctggacggc tgaaactccc tggcaaatgt tgattgaaga ggctcgtctg
    34861 atcgcacctg gcgcggatgg cgtgaaaatg cagtgtgatt tattgtcgtg tcagaacgct
    34921 ggctggcaag gagtgacgct taataccacg cgggggcatt tctaccgcgc ggcgctggaa
    34981 gggttaaccg cgcaattaca gcgcaatcta cagatgctgg aaaaaatcgg gcactttaaa
    35041 gcctctgaat tattgttagt cggcggagga agtcgcaaca cattgtggaa tcagattaaa
    35101 gccaatatgc ttgatattcc ggtaaaagtt ctcgacgacg cagaaacgac cgtcgcagga
    35161 gctgcgctgt tcggttggta tggcgtaggg gaatttaaca gcccggaaga agcccgcgca
    35221 cagattcatt atcagtaccg ttatttctac ccgcaaactg aacctgaatt tatagaggaa
    35281 gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc gaactattga aagtgctggc
    35341 agagatggga catggagatg aaattatttt ttccgatgct cactttcccg cccattcgat
    35401 gggaccgcag gtgatccgcg ctgatggcct gttggtgagc gacttgctcc aggcgattat
    35461 cccgttattt gaactggaca gttatgcccc gccgctggtg atgatggcgg cggtagaagg
    35521 tgacactctc gatcctgaag tagaacgacg ttatcgtaat gcgctttcac tacaagcccc
    35581 ctgtcctgac atcatccgca tcaatcgttt tgcgttttat gaacgggcgc aaaaagcctt
    35641 tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat attcttttaa aaaaaggggt
    35701 aacaccgtaa tctcataccg gtacgcccgc atgacgcggg cggttatcga atgatggggt
    35761 gaaaattatg aaagcggcac gccagcaagc gatagtcgac ctgctgctga accataccag
    35821 cctgaccacg gaagctctct ctgaacagct aaaggtcagt aaagaaacca ttcgtcgcga
    35881 tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat catgggcgcg ctaaatatat
    35941 ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc cggctgaaaa gccattatgc
    36001 gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt gaagaaggga tggtgatagc
    36061 cttagacgcc agttcaactt gctggtatct ggcacgccag ttgcctgaca tcaacattca
    36121 ggtcttcacc aatagccatc cgatttgcca tgaactcggt aaacgcgaac gcattcaact
    36181 gatcagttcc ggtggcacac ttgagcgcaa atatggctgt tacgtcaatc cctcgctgat
    36241 ttcccaactt aaatcgctgg aaatcgatct gtttattttt tcttgtgaag ggatcgatag
    36301 cagcggcgca ctgtgggact ccaatgccat caacgctgat tacaaatcga tgctattaaa
    36361 acgtgccgcg caatcgttgt tattgattga taaaagtaaa tttaatcgtt caggggaagc
    36421 ccgcatcggg catctggatg aggtaacgca cattatttct gatgagcgcc aggttgcaac
    36481 ttctttggta acagcctgac ggaaagggta gcaggccgga gacgacgccc cggccttgcc
    36541 tgttatcgct cgtcgcgacg accacccacc gcagcccaga tgcggcggac gtgcaccgtc
    36601 acttcttcgc gatcgtgata caactgccgc gcctgaatct gagcatttat gccatgttca
    36661 tcaagctgtg cctgaatata cgccagattg tgtgacactt cttcgtagcg ttttttcatc
    36721 ggcagtttga ggttgaaaat ggtttcacgg caccagccat taaccagcca ctgcgccatc
    36781 aatgccgcaa ctttcgccgg tttttcaacc atatcgcata ccatccagga gatattgctg
    36841 cgtgtcggac ggaatttgaa accgtcttcc cgcagccacg tcacctgtcc ggtatccatc
    36901 agactttgcg ccatcgggcc gttgtcgacg gaataaaccc acatgttgcg cttcaccagt
    36961 tggtaggtcc agccgcctgg gcaagcgcct aaatccaccg cccacatccc gttcgccagg
    37021 cgttcatccc actcatccgc aggaataaac acatgaaatg cctcttccag tttgagcgtg
    37081 gaacgactcg gcgcatctgc cggaaatttc aggcgcggaa tgcccatata gaacggagaa
    37141 ttattgttgc tgtatgagta accggtatag cagcagcctg gtgcaataaa gaatacatgc
    37201 acaaccggac gcttcggcgt ttcatagttc gccagcacac ccgcatcgcg caaggcagcg
    37261 cgtagcggca cggtgaattt acggcagaat ttcagcaact ctttgctttc gttggtatcg
    37321 gcaacttcaa cacgcagttc accgcccttc tctactacac cctgtaacat gccgacaatg
    37381 ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt cccccaccac aaaccactgg
    37441 cgggcaaaaa ttaatgaact gaacggcagc tcacggatta acttatcgcc atcatcaggt
    37501 tgataacatt cataaatgac ataacccgca ttctctttca cgcgggcaaa accgaaaatt
    37561 tcccgctggc cggctttatc ggtaatttct gcggcgcact ctttttcaaa gcccggacgg
    37621 cacagcaata caaccttatt catgacttac gcccttgcgc tttaaacgga tagcaccaac
    37681 taacattaac gcccagcccg cgaggaagct cacgccgcca accggtgtga caaatgccca
    37741 caaacgcaga tgggacagcg ccaggcaata aaggctgccg ctgaacagca ccgtgcctaa
    37801 cgcgaggaaa acgctactcc agtaaaacca gatactgatg cgacgctgca ttgccaccgc
    37861 cagacctaag atcgccagcg tgtggaacgc ctggtattcg agaccggtct ggatccagcc
    37921 catctcaacg gcccccatgg ttttacttaa cacatgcgcg ccaaaagcgc ccagagccac
    37981 aaaaatgaag ccgctaatgg cggcaaaaat cagcataaaa cggctggtca tggtcgtacc
    38041 ctacgtaaat tattgttcat aacgaaagcg gaatttttct tgttcagcag cggctttcgc
    38101 caggatccat tggcgaaagg cggctatttt acccagttct gcctggctgt catgacaaac
    38161 cagataaaaa gcgtttttac tgaccagaac atcattaaac gggcaaacaa gacgtccggc
    38221 ctcaatttca gattgcgcca tcacgttatt tgccagcgcc actccctgcc cgtggatagc
    38281 cgcttgcagc accatggcgc tatggctaaa aattggccct tgctgaacgt tgatatgatt
    38341 taaccccaac tgtcgggtat atgtctgcca gtcacggcgt gacgcatcat gtaataacgt
    38401 atgtttagcc agatcttccg gggtcttcaa gggtttttcg ccagtcagca gtagcggcga
    38461 acacaccggc aataaatatt cggcgtaaag tttttccacc cgtagccccg gccagttgcc
    38521 ccgaccataa aatatcgcca catcaacatc atccgccagc ttatcttcct gacgatcaac
    38581 cgcctggatt cgaacgtcaa ttcccggata agctgaatta aagctggaaa gtcgcggaac
    38641 caaccaatga atggcgaaac tggggagtaa actgaccgtc aacgccccct tggcgctacg
    38701 ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat atctctttga tatcgaggaa
    38761 atagctttgc ccttcctcgg tcagcaggag tgaacgatta cggcggcgga acagttttag
    38821 ccccaaaaaa tcctcaagag acttgatttg atgacttact gcggcctggg tcacaaaaag
    38881 ctcttctgct gcgcgagtga aacttaaatg gcgtgctgcg gcatcaaaaa ctcgtaaggc
    38941 atttagcggt ggtaatcgtt tagacatggc ttttaaactt tgatgttaaa tgaatttaac
    39001 aattagatca cgctgtgtaa cctattagtt tttttaatct gagccattat aaattgtccg
    39061 ttgagcttct accagcaaat acctatagtg gcggcacttc ctgagccgga acgaaaagtt
    39121 ttatcggaat gcgtgttctg gtgaactttt ggcttacggt tgtgatgttg tgttgttgtg
    39181 tttgcaattg gtctgcgatt cagaccatgg tagcaaagct accttttttc acttcctgta
    39241 catttaccct gtctgtccat agtgattaat gtagcaccgc ctaattgcgg tgcttttttt
    39301 ttaccttgcg atcgcgaatt actgatccag ttcgaccatc tctttcacgt cggtacggtt
    39361 gatctgctgt ttgttgccat tagcgtcttt atacgaaatc ataccggtat cgttatcagt
    39421 ctgtggcttg ccgtcagaga cgatggtacg tccgtcattg gtgtgcatca catagttcga
    39481 accggaacag gcgctcaggg caaaagtcag catacaggca gaaataattg cggcagtctt
    39541 tttcatcatc ttctccttaa agcgattaat aaatcttaat agtattgatt aacaggctaa
    39601 aattaacgcc taacactatt cagcatatgt tactgacgcg gcttcgccag gatatccaga
    39661 taattctgat ggttagcact ctccttgtat caaagtgaat tttgcgtcac gatcggtgca
    39721 tcaagccgag gagtaccatg aacgttttta atcccgcgca gtttcgcgcc cagtttcccg
    39781 cactacagga tgcgggcgtc tatctcgaca gcgccgcgac cgcgcttaaa cctgaagccg
    39841 tggttgaagc cactcgacag ttttacagcc tgagcgccgg aaacgtccat cgcagccagt
    39901 ttgccgaagc ccaatgcctg accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat
    39961 tactgaatgc accggatgat aaaactatcg tctggacgcg cggcaccact gaatccatca
    40021 acatggtggc acaatgctat gcgcgtccgc gtctgcaacc gggcgatgag attattgcca
    40081 gcgtggcaga acaccacgcc aacctcgtcc cctggctgat ggtcgcccaa caaactggag
    40141 ccaaagtggt gaaattgccg cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag
    40201 aactgatcac acctcgtagt cgaattcttg ctctgggaca aatgtcgaac gtcactggcg
    40261 gttgcccgga tctggcgcga gcgattacct ttgctcattc agccgggatg gtggtgatgg
    40321 ttgatggtgc tcagggggca gtgcatttcc ccgcggatgt tcagcaactg gatattgatt
    40381 tctatgcttt ttcaggtcac aaactgtatg gcccgacagg tatcggcgtg ctgtatggca
    40441 aatcagaact gctggaagcg atgtcgccct ggctgggcgg cggcaaaatg gttcacgaag
    40501 tgagttttga cggcttcacg actcaatctg cgccgtggaa actggaagct ggaacgccaa
    40561 atgtcgctgg tgtcatagga ttaagcgcgg cgctggaatg gctggcagat tacgatatca
    40621 accaggccga aagctggagc cgtagcttag caacgctggc ggaagatgcg ctggcaaaac
    40681 gtcctggctt ccgctcattt cgctgccagg attccagcct gctggccttt gattttgctg
    40741 gcgttcatca cagcgatatg gtgacgctgc tggcggagta cggtattgcc ctgcgcgccg
    40801 ggcaacattg cgctcagccg ctactggcag aattaggcgt aaccggcaca ctgcgcgcct
    40861 cttttgcgcc atataataca aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg
    40921 cgctggaatt attggtggat taatgacgaa cccgcaattc gccggacatc cgttcggcac
    40981 aaccgtaacc gcagaaacgt tacgcaatac cttcgcaccg ttgacgcaat gggaagataa
    41041 atatcgccag ttgatcatgc tggggaaaca gcttccggct ttgccagacg agttaaaagc
    41101 gcaggctaaa gagattgccg gatgcgaaaa ccgcgtctgg ctgggatata ccgtggctga
    41161 aaacggtaaa atgcacttct ttggcgacag cgaagggcgc attgtgcgcg gcctgctggc
    41221 ggtgttgttg actgccgttg aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct
    41281 ggcattgttt gatgagctgg gattgcgtgc gcagcttagc gcctcacgca gccaagggtt
    41341 gaatgcgtta agcgaggcga ttatcgctgt ggcgaagcag gtttaagcac agaaccaggt
    41401 cggataagac gttcgcgtcg catccgacct gattgtttac ccctgacggg ccgctttcgc
    41461 catcatcttc ttcagcgcat gagaaaccgc aacaaaacca aaggtggcgg tcaccatcgt
    41521 cgccgcgcca aatccagatg cacaatccat ccgcttcggc ccttccgccg tggctttcat
    41581 agcacacacc gtaccgtctg actgcgggta caccaatgct tcggtagaga acacgcaatc
    41641 cacgccgagc ttacctttac tgtttttcac tatgccaaaa tcgcttttca ggcgctcacg
    41701 caacttcgcc gccagcgggt cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt
    41761 cgggtcaatc tgcccacccg cgccaccagt cgtgaccagc gggattttat tgcgccgaca
    41821 ataagcaatc agcgccgctt tgggccgtac actatcaatg gcatcaatca cgtacgaata
    41881 acccacgctc atatactgcg ctacgttatc cggcgtcacg aaatcatcca ccaccgttac
    41941 acggcactcc gggttaatct ggcgaatacg ctccgccata acttccgctt ttgccagccc
    42001 aacgttatcg cgcagggcat gaatttgccg attggtattg gtgacgcaca catcatccat
    42061 atcgataagc gtgattgcgc caatccctgt gcgcgccagc gcttccgccg cccaggaacc
    42121 gacaccgcca atacccacca cacaaatatg tgcatcggca aacagttgca gcgctttttc
    42181 accatacaga cgcgcagtgc cgccaaaacg ctgacgccat gcatcactaa ttaccacaga
    42241 catagcacct cagaattaaa aagggcgagg ttatccctca ccctaaacgc ataaatgcct
    42301 gatgcgctac gcttatcagg cctacataac tcctgcaata ttttggattt gcatgatttg
    42361 taggccggat aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt agtcaataat
    42421 caggatgtga ggttacccct caccctgtca tatccgtaaa aacggcatac agaatatcac
    42481 atcagccgct aaagacgtta cctgcgcccg gagccgtttt cagcacccag acacgtccat
    42541 agtggttgta ccaacctgcg cggtgcccgg cttccggccc gatcccttga tagatatcga
    42601 agtgttggcc tttgattgcg ccaccgacat ccagcgccac catcagacgc aattcgtact
    42661 gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag gtagtacctg
    42721 gcggaataat tgaacgatcg gaggcaacag aagcacggcc aaccagcggc accgcacttg
    42781 cccctttcac cggtgcaaaa gattgcggtt taaagaagac gaaagacggg ttctgttcca
    42841 gcagctcgcg aacctcggct tcactgtgtg tttcgcccca gtgacgaatc gcctgcatcg
    42901 acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg ctgcgatagg
    42961 catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca ccaaagtcga
    43021 tatacccact accctgcaca tccataatga agttatccat cagggagtta ctgtaagcga
    43081 gaatatattt atcactcaat gcccccgcgt agatctccgc acgagacggc agacgaccac
    43141 gttttggcgg catacggtaa atcggatact ggaactcgcc ctggcgggta tggcgcgcct
    43201 gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg gcaccttcca
    43261 tctgccaggc atcaatgccg aactggcgca tattgcgggt atcaccgcct gcgcgcagcc
    43321 actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt gacgaattac
    43381 ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca acggcatctg
    43441 gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc tgtccgcgat
    43501 cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata agaaggtact
    43561 ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg caacgaagat
    43621 aacaaaccgc cgcgtttaat gaaatgagca gcctctccct gacgcaaatt ttgcacaaaa
    43681 aataggcttt agtgatttgt ttttgttcaa aatcatgccg aatccgtgat cggggtaaaa
    43741 aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc ggggtggagc
    43801 agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca
    43861 accaattatt gaacacccta acgggtgttt ttttgtttct gctctcccat aaaaaaagcg
    43921 ccattcagcg cctttttatc atccccttct cgccagcgtc gccccatcgg caaaatacgc
    43981 tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaggtcgccg ttttcagttt
    44041 acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg gaatatcagg
    44101 cgctttcagc acggcaaacc cggcctgttc aacctgattt ttatgcagct tgttgatttt
    44161 accgagctta ttcagcaccg ctttaccaaa cttaaggctg tcggcaatgg tcagcgactg
    44221 caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca cgccaccaat
    44281 caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg ttgcaccttt
    44341 ggttgagagc gcaaacacag aggaaccgct cggctggcga ctggtaaagg cgtcggcatg
    44401 gatagagaca aacaggtcgg cacgctgttt ctgggctttt gctacgcgca cttgcaacgg
    44461 aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt tctcgatcag
    44521 agagcgcaaa cggcgagcta tttgcaatac cacgtctttt tcgcgcgttt tgtatttccc
    44581 caccgcaccg gagtcttcgc cgccgtggcc agggtcaagc ataataacaa tcggacgatc
    44641 gcgccctgct ttacccggtt gtggaccact ttgtgctggc ggcacctgct tttcgaggtc
    44701 gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct gtgcattagc
    44761 cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa gggcaaacag
    44821 ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg ggtcaaattg
    44881 cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg ccatcccctt
    44941 gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttccg ggttactcaa
    45001 cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg tgtagctgga
    45061 cgcaggccag acgcgcaccg ccacgacctg gctgaccgca gccagactga cctgacttac
    45121 gctcaatagc cacatggcac ccgcgccttg cagtaaacga cgacggctga ttgcagtgtt
    45181 ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa gtaaacgact
    45241 aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa cggtaaacat
    45301 tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa tttacacttg
    45361 caccctgcga aaaaacagaa taaaaataca ataatttcga ataatcatgc aaagaggtgt
    45421 gccgtggtaa aggaacgtaa aaccgagttg gtcgagggat tccgccattc ggttccctat
    45481 atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc cattgagcat
    45541 gagaatttct ccagtatcgt taatgatatc gggttgctgc acagcctcgg catccgtctg
    45601 gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca tcaccacgaa
    45661 ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact ggtgaagcag
    45721 gctgcgggaa cattgcaact ggatattact gctcgcctgt cgatgagtct caataacacg
    45781 ccgctacagg gcgcgcatat caacgtcgtc agtggcaatt ttattatcgc ccagccgctg
    45841 ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat tgatgaagac
    45901 gcgatccatc gtcaactgga cagcggtgca atagtgctaa tggggccggt cgctgtttca
    45961 gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact ggccatcaaa
    46021 ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgtcactaa tgacgacggt
    46081 gatattgtct ccgaactttt ccctaacgaa gcgcaagcgc gggtagaagc ccaggaagag
    46141 aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa agcctgccgc
    46201 agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag atggcgcgct attgcaagag
    46261 ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga gcagattcgt
    46321 cgcgcaacaa tcaatgatat tggcggtatt ctggagttga ttcgcccgct ggagcagcaa
    46381 ggtattctgg tacgccgttc tcgcgagcag ctggagatgg aaatcgacaa attcaccatt
    46441 attcagcgcg ataacacgac tattgcctgc gccgcgctct atcctttccc ggaagagaag
    46501 attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc acggggcgag
    46561 gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa attatttgtg
    46621 ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccagt ggatattgat
    46681 ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa agtgttgatg
    46741 gcggatttgg ggtaagcgat gaaaatcgcc ggatgcgaca tgcgtaacac tcgtgcgtcg
    46801 catccggcaa ttacgtttat ccccgtgaac taaacaacgc cgccagaccg ctgcgccgct
    46861 cggtgcgcgt ggcaattgcc gcacttaaaa tgcgctcatc ggcatacagc gacagacggc
    46921 gacgcgcgcg ggtcaccgcg gtgtaaacca gctctcgtgt tactaccggc gtgcgttggc
    46981 tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt accgtcatcg
    47041 cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata ttgccgtccg
    47101 gcatcgcaaa ccagacgcgc gtcccctgcc cacgatccag agcgataccg atatcgccat
    47161 taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct tcgtaccaac
    47221 gagagtgcgg attacgatga attttgcgct tctgttgcat aaactgctca attcgctcat
    47281 tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg tactcattga
    47341 acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga taacgtccgt
    47401 aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt aaaagccgtt
    47461 tttcgatatc agaaaaatcc tgctgaaaaa cggttttcac tgccgtttta tcaccacggt
    47521 tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag cttttttgca
    47581 gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc agttcctgcc ggaacgtgag
    47641 ttcccgtcag gcggctcaac tgcccggcac gctcggcggt aaagccggca ttggcataag
    47701 cgcagatatc gcccagcact gccccagcct caaccgaagc cagttgatca cgatcgccga
    47761 gaaagatcac tcgcgcatga tcgggcaagg cgtcgatcag tctcgacatc ataggcagat
    47821 cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta ccggcatgat
    47881 gacgtaaacg ctggctaccc ggctgcgcac ccagcaatcg gtgcaaagtg ctggcatctt
    47941 ccggaatgcg tttcttttgt tcatcggtca gcggtaactg gcgcaaagcc ttgccgagag
    48001 attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata cggcagcgtt
    48061 cgccgtctgc catttgaatt aacgctgcca gcaacttcgc tacggtggtc gttttaccgg
    48121 tgccagggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc gccgcaactt
    48181 tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc gccagtagag
    48241 cttcatcaac ctcaatggca tgattcactt cgttgaaaaa gcgtgccact gtgcgctcgt
    48301 tacaccacat gcgattcaaa taaagacgat caccacagag gatcatcggc gttggttcat
    48361 ctccccggct gaccgcttgt gaagccagca agcattcttc ccaattttgt aactcaccga
    48421 tttcactgac acaggttgct aacagcggat gtgactcctc gttattttcc agtcgtgaga
    48481 gcggtaaaca gacatgccct tccccggcgt cgtggcttaa cagggctgcc gcgagggtga
    48541 cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc gggcgtagct
    48601 gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct cctccagggt
    48661 cataccggca aacatctcat ccatcaggtc aatcaacccg gcgttgggtc gggttgtgta
    48721 aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga taacgccgcc
    48781 aaagtgacga tcatagtcgt aatcagcaat gcgatggcgc agataacggt gcagcgccag
    48841 ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgccgctg ccatagcctg
    48901 ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga gcaggtaata
    48961 acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc gtacctgcat
    49021 gaactccagc ggcgggcagc cagcggatag cggatcaaac tggcgaatta gtgcatcaag
    49081 ctggctggcg atgagcggtt cactaatcgg caggtaaaac tccatctcca cctgtttatc
    49141 gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct ggaggacagc
    49201 cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga gttccagttt
    49261 ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa acaaactgtg
    49321 caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg tcggttcttc
    49381 aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat cctgagcgat
    49441 accgtgacca cgctgctgca aaccggagta gctggtgaca cgccagttat cgccgggcaa
    49501 tcgttgtagt gtcctcgcat tcagttctgc agttatagca tcattaacct gccagggctg
    49561 gttatcaccc gtttgtgccg tttgccaggc aatatcatca tcgcataacg cttcaataca
    49621 ggtgcgaagt cctgccgcat cttgcggttc ccctttttgc agcaaacgcc cgagcgcact
    49681 ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg gtgcaacgcc
    49741 gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca gatcttccgc
    49801 cagacgttcg gcctccgcga gatcgacact ttctggcgca gcgttaagat ccagaacagc
    49861 ctcaaacgag tggcgatcgt gatagaacgc ctgatcctgg acgcggaaat tggtaataaa
    49921 tggcaaccag accagcggat attccagccc ttttgattta tggatcgtga caatctgcac
    49981 cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt ctggctcgag
    50041 gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg ttccggcttc
    50101 ttgtagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc ctgccgttgc
    50161 cagcaagttt tcagcaatgt tacgcgccga catcagcgcc cgcagcatcg gcataacgcc
    50221 acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat cccacgcatg
    50281 ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg acgttgccag
    50341 cgcactgcgc agggtgttct cacgttcggg agtcatcacc gcctgcaaca gccaaagcat
    50401 ctcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa cggaagggat
    50461 ttccagcagc gttaaggcat cgcgcacctg agcggcctcc tggcggctgc gcaccagcac
    50521 actgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca acgcttcgcc
    50581 ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg ccatggtact
    50641 ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca tcgcaggctg
    50701 cgtttcacct ttaaacacaa aacgtaacgc ctgatttttc ccggctgatt tcactggaat
    50761 aaaaggaatt tcgcgaaaca tgaacgcgtc atcagtctga ctgaaaagct tattcacgct
    50821 gttcaccatt cctggtgcgg agcgccagtt ggtatctaaa gtgtagtggg cgtgaacttc
    50881 gctacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata tggcctgttt
    50941 cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac ggcgaaaaat
    51001 tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg ggaatcgcgt
    51061 acggatcgcc gctgccaaca cctctccgct ttcgctacgc agcgcggaat cgagccgact
    51121 taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta ctgtttcgcg
    51181 gatctcagcc aatgcgcggg tgatcaccag atcgcggatc gacaatggtt ctgcaagcag
    51241 ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggctt tcgtgcgatc
    51301 ttctaagaaa cgctgagaga atttttccag cgactccggt aactgataac tgttggtctc
    51361 ttcttctgcc caggcgctga tcttgtcgat ccatttagcc tgattgctac ggttaaactt
    51421 gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccaccg cgtcgcgcca
    51481 ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag ccagcgtttc
    51541 atcatcgggc ggcggcgctt tgataaccgg cgcttcgcct tgcagataac gattaatatc
    51601 gcgcagcaac gcctgcggtc ctttccaggt ttcaaagacg acctgggcaa tttcacgcgg
    51661 cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta gcagagactc
    51721 atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca ggttgagcat
    51781 gcgctggcaa aaaccgtgaa tagtaaagac tgccgcttca tccatctgcc gttcggctaa
    51841 caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc gttcgtacag
    51901 tggattgtcg gtggtttcac gcaaacaggc gatgcgcaac tcgtggatat tgctacggat
    51961 acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca gttcttcaac
    52021 ggtcagcggg cggggaaagg cggcagaacc gcctagtcca agtaacaggc gcaaatagag
    52081 cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc gctcgccctg
    52141 taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt tcactcatca
    52201 ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct tcaggatgca
    52261 catattcggc tttcccgttc tggctaccgg aaatctgcga cagaatagcc atgccttgcg
    52321 gctcgaccac cgcctgatgg aagaaatcgg ccagtttttg cggcgtcagc agttttatct
    52381 gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa tctttactta
    52441 acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta attaccgcct
    52501 gctggatttg cgcaaactct tctggcttca tcgtccgcaa ttttgcctct gcggttggga
    52561 aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt tgcaacagga
    52621 agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca tagcccaact
    52681 gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc aacagagagc
    52741 tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct gccagtgcgg
    52801 agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg accacgacat
    52861 ctttgttacg acaccactcc gaaccatcag cgcccaactg tttttgcaca tcgcgtgcca
    52921 gcgttgttgc ctgggcttcg gtcatgttgc caataaccat aaactctggt cgagcccctg
    52981 attttaaggc gtcgcgatag gccagcacct ctttcaaagt gattgaaggc aaaattttgc
    53041 gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc ataatcgcct
    53101 gctcgaacgc tttgcccttt tctgcagaat ccatcatctg gttataccag gacttcgcct
    53161 gctcaagctg atcttccgta gcggtatagc taaagtagcc ctccagcaga gcctggaaca
    53221 gctgcggcag gcgctgggtg taaccattgg cattaaccat aaggccgttg ttagcgttgg
    53281 tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc gccagtccgg
    53341 caagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg gctttcgggt
    53401 tacgcaaaat caggctgaca tcagctttgg gttcgctgga aaaataacgg cttggcgcat
    53461 acaccacacg cagattcgac tcatcaacaa tcagctctgg atggtcatat ttcttctctg
    53521 acttaatcag cgagaaatcg tcaggaatat aggggttaag ctctggcaaa gagagcgcaa
    53581 tgtcggctgc ttttttctgc cagtcagcga aagtttgtgc gctaatttta tcgacctgat
    53641 acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg atataccaga
    53701 tacgcgcatt ctgcggcgtc atcatcgcca ggcgttcttt tactgcttta gcgtcgtacc
    53761 gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc atggtatctg
    53821 ccagccattc gacgtaatcc atatcacggg tgattgacgg ataacggaag tcgatatcca
    53881 gcacattcgc cagttcatcg aagtattgtt tatcaatccc tttttcgcgt aacagattga
    53941 ggtagctaaa aatggccgcc acaacctgat cgcgattcgc cagaccttta tcggttaaag
    54001 acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggaa ttggcgctaa
    54061 tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg ctgcgattac
    54121 cgatcagata ggtaatcaat tcatccgttt tactacggaa cttcgctgaa ttgttatcga
    54181 tgcgaaactc aacgcgcaga actttacgcg gcagcgccgg gacgtaatga atgataatgc
    54241 ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc tctttgttca
    54301 gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt ttattgctgt
    54361 aaataaccgc cttcatcaga ttggcggaat agtacttctc gtggaaatct ttcagcgcct
    54421 gctgtaccgg atttccgggt ttgtcgctta aagtttcgag gttaccacca gaaaactttg
    54481 aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc atcccgtcac
    54541 gcgtacgcgc catggttaat tcggcattca ctgcattacg ttcacgttcg gcgtatttct
    54601 tgtcgagcaa gggttctgca atagcgtccg ccaggcggtc taccgcaccg ggcaaggcgt
    54661 cgttctcaac ttccagatag aaagccgtgc gatacggtgc cgtgctggca ttgtgactgc
    54721 cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt ttcgacccca
    54781 tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga tcttccagcg
    54841 acccaacggg cactaccagc gccgagagcg atttaaccgc ctgcggatca gaaaccagca
    54901 agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct ttatcacttt
    54961 tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaag ggtgcccaaa
    55021 gggcaacaaa caacaataat gctttgaacc aggtgctgct gggcattcac ggacctcata
    55081 atcaacttta ttttctggcc agattcaaca ggttaacgca aatcatctgc cagaatttaa
    55141 tcttgtgctg cacgagtcag cctatgtcta tatatccatc agtccgtgac tggtgcgcat
    55201 cataaagcaa gcagatatat tgcgcaattt ttatacagca ctcatgactg attaaagcga
    55261 aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt ctctggtgtt
    55321 aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac catcatgttg
    55381 ccttcgtaag cctgaaggaa tttcgtacgg gctttttgta acgtggaatc gtcatccagc
    55441 atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact ttcaggtaac
    55501 accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga gaggtaatgc
    55561 aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc cgtctttgcg tagaaaaagg
    55621 cgactttcac cattaccacc gctggcacag tagacaaggt gttccagcca aagttgcatt
    55681 ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg ctgcacctgc
    55741 ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat actctgcccc
    55801 ggctggcgac aggcaataac tctgtcggca agctgctgca tctcctggca ctgtgtttcc
    55861 cagaaaattt caccaaaagc gccatgcggt aaatcccctg ccgcccggaa gcggcggaac
    55921 aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt gatttgataa
    55981 cggctaagcc cttccagaat aaatggctcg gtgtcgggga tttcgctgtc ttcagtacgg
    56041 aagttcacct gcaaacgcat ctggaagaac gcccgtaccg gatgtgccca gaatcgttgt
    56101 agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac aaattcagaa
    56161 tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct ttgtcgttcg
    56221 cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt aagatgcgct
    56281 tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag ataatggctt
    56341 tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc actgttatcc
    56401 tgaatagaac gcccgatata gctgatatag agtttttgct gcgcggaaat taacgcttcc
    56461 aggaacagat agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg tttctggctc
    56521 atcaggtcaa agcccaatgg cgcaagctgg cgtggataaa cgccgtcgtt cattcccagc
    56581 aggcaaacca ctttgaatgg gatggaacgc attggcatca gagtacaaat gttaaccggt
    56641 ccggcgagaa aacgctggct gatacgttcc tgatccaggc gctgtgccag ttcatcacgc
    56701 aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc gataatcgcc
    56761 tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg caggaagaag
    56821 gcgttgagca tatcgcgaca aaccggcaac cactcttcca gcggacgctc ctgcgccagc
    56881 ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac tagttctgca
    56941 attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg cgcgctctcc
    57001 atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg tccggtggcg
    57061 gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc agactcgttg
    57121 acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc cgccagcacc
    57181 ggcacatcca gtaatgccag aacgtcctcc gacacaaagc gactgtcagg cagtgataac
    57241 aggctgataa acgcttcaag tacaggatgc gactgccgcg cccgacggtc ggaaatggcg
    57301 taaggtaggt aacgatccgc aggtgcgcta ccaaacacag cctgaataaa cggactgtag
    57361 ctgtcgatgt cggccaccat cacgatgatg tcgcgcggag tgagtgtcgg gtcttcctcc
    57421 agcatcgcca gcaggcgatc gtgtaaaact tcaacttcgc gctgcgggct atggcaaacg
    57481 tggaaggtga tactgttatc cagtggatca agcaggcgtt tgttatcgct acgggaaaac
    57541 tcttcgatat tcacacccgc aacagcgcag ttttcaagtt ccagaatgtc agactgaatg
    57601 ttatgcagca ggttatctgg cgtgacatcg acaaaggcgt ccagctcctg gctgccctcc
    57661 agatcagaaa ggagataaat gtagtcgcgc ccaagcttgc cccatgaggc cagcagcggg
    57721 ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc gctgtcgcga
    57781 aatagcggta attcgcgatc ttcaaaactg tgtcggcgct ggcgagtcag cagtttcgcc
    57841 agataagcag gatctttaat gtcgccccag taataacggc aggggttggt aaacaggaga
    57901 tggatttcaa tatgtttacc cagcgcctgt agcgcctgga gataaacagg cggtaacgcg
    57961 gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt cgcggactcc
    58021 agcgtttcga taaagcgctg ataaagattg gcgcggtgcc agcgcggttg cccgagttcg
    58081 tcggtatatt ccaccaacgc cttccacaac ggcgcttgcc aggcctgcgc ctctccaagt
    58141 ccttctacca gattcccggc ttcccactgt gccagccagt ccgggcgata gaccagatac
    58201 tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt gtcgctatcg
    58261 tcagtcagat aatgccgcaa cagggtaaag tcttcgcgat ccagcaattg cggcaacaga
    58321 gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg gatttccggt
    58381 aacacccgga cgaacatatc ccagataaag ctcgcaggca gcggaaaatc aatgtttgcc
    58441 gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc ggtactttgc
    58501 accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac aataaactcc
    58561 atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat agcggctcct
    58621 gactactgac gattcgggca atgcaggcgg gtcatctcgc cttctctgcc cccgggtgaa
    58681 actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg gttgacctgc
    58741 cagttggcag gtggcgaaat cgcacgcagt tgcgtttgct gccagccatg ccgccagagc
    58801 tgttggtact ggtttctgct ggcaagactg ttcattaatg ttcgctggaa acccgataac
    58861 gcagtgacaa tcatcaccat caacaccatc gccaacatta cctccggcag gctaaagcct
    58921 tgttgattct tcagggaagc tgacataacg ccctctcttt cagcggacaa aaatcgctcc
    58981 agccgcgtgg cgaaaaaaca atgtttccat cgatgacttc acctgttcgc cacaacgaaa
    59041 cgccttcata accggcaatc aataaggcct cattatctgc caataaacgc agacaaaccc
    59101 gggcaccggt ggcagcatac agtaagcact gaactgcggg ctgcgtctgc caggagtgca
    59161 tttttcccca ctccagcgcc gactgaacga tggcctggcg gcgcaatgac tgactttcca
    59221 tgctcacgcg agaggcaaaa ctgcgatcct gttggctcat tccttgtaat agcaagctac
    59281 ccaaaatcag cagcatcagg accagagcca gtgacgaaac acccttttcg cggttcacag
    59341 gttggatcct gtcacgctat agctggcatc caccacggtt tgcggatcag ccttgctggc
    59401 agcgtgcata ttaaccgtca acaccggcga gaaaccgctg acatcctgtc gggtaacctg
    59461 aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct taccttcaca
    59521 ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc caatctggtc
    59581 ggactctttt accggttcgc gatcccagat accgttactg ttcgcatccc actgcacaat
    59641 gacacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc catgacaata
    59701 acccgccctc tggagatgct tcgcaacggt aaatacccgc agccagattt catcttccag
    59761 cgccagctta cgggtgctcg ttaaactgtc acgctgtaac gcaggcagaa agcgtgctgc
    59821 ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca gagaaaaacc
    59881 tagctctttt acaggcatcc ttctgtttct ccttgctgac aaagccggag tcttccccac
    59941 ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg cccggcccat
    60001 gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg
    60061 ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc cccagcagcg
    60121 gaactcacaa ggcaccataa cgccccctcc ctgataacgc tgatactgtg gtcgcggtta
    60181 tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctggct ggcggtttgc
    60241 catagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc gcttagcatg
    60301 accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg tgttttcatg
    60361 ccggcagtat ggggcgagga gaaaaaaaga cgagggccag tttctatttc ttcggcgcat
    60421 cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga
    60481 atttaacgga gggtaaaaaa aaccgacgca cactggcgtc ggctctggca ggatgtttcg
    60541 taattagata gccaccggcg ctttaatgcc cggatgtgga tcgtatcctt caatctcaaa
    60601 gtcttcgaaa cggtagtcga agatggattc gggtttacgt ttgataatca acttcggcag
    60661 cggacgcggt tcgcggctta attgcagatg agtttgatcc atatggttgc tgtacaggtg
    60721 cgtgtcgcca ccggtccaga caaaatcacc cacttccaga tcgcactgct gcgccatcat
    60781 atgcaccagt aacgcgtagc tggcaatgtt gaacggcagc ccgaggaaaa cgtcacagga
    60841 gcgctgataa agctggcaag agagtttgcc gtctgccaca tagaactgga agaatgcatg
    60901 gcacggtgcc agcgccattt tatccagttc gcctacgttc cacgctgaaa caataatgcg
    60961 gcgggaatcc gggtcgtttt tcagctggtt caataccgta gtaatctggt caatatgacg
    61021 accatctggc gttggccagg cgcgccactg tttaccatac actggcccga ggtcgccatt
    61081 ttcatcggcc cattcgtccc agatggtgac attgttttcg tgtagataag caatgttagt
    61141 atcgccctgc aggaaccaca gcagttcgtg gatgatggaa cgcaggtggc aacgtttggt
    61201 tgtcaccagc gggaatccat cctgcaggtt aaaacgcatc tgatgaccaa aaatggaaag
    61261 cgttccggtt ccggtacggt cgtttttctg tgtgccttcg tcgagcactt tttgcatcag
    61321 ttctaaatac tgtttcatgg ttcctcagga aacgtgttgc tgtgggctgc gacgatatgc
    61381 ccagaccatc atgatcacac ccgcgacaat catcgggatg gaaagaattt gtcccatgct
    61441 gatgtactgc acccaggcac cagtaaactg cgcgtcgggc tggcggaaaa actcaacaat
    61501 gatgcgaaac gcgccgtaac caatcaggaa caaacctgag acagctccca ttgggcgcgg
    61561 tttacgaata tacaggttga ggataataaa cagcactaca ccttccagca acagctcgta
    61621 aagctgtgat gggtggcgcg gcagcacacc gtaagtatcg aaaatggatt gccactgcgg
    61681 gttggtttgc agcagcaaaa tatcttctgt acgggagcca gggaacagca tggcaaacgg
    61741 gaagttcggg tcaacgcggc cccacaattc accgttaata aagttgccca gacgcccggc
    61801 accaagacca aacggaatga gtggtgcgat aaaatcagag acctggaaga aggaacgttt
    61861 agtacggcgg gcgaagataa tcatcaccac gataacgcca atcaggccgc cgtggaagga
    61921 catgccgccg tcccagacac ggaacagata cagcggatcg gccataaact gcgggaagtt
    61981 gtagaacaga acataaccaa tacgtccacc gaggaagacg ccgaggaagc ccgcatagag
    62041 taagttttca acttcatttt tggtccagcc gctgcccgga cgattcgccc gacgtgttgc
    62101 cagccacatt gcaaaaatga aacccaccag atacatcagg ccgtaccagt gaagcgctac
    62161 gggtcctatt gagaaaatga ccggatcaaa ctccggaaaa tgcagatagc tactggtcat
    62221 ctgtcaccac aagttcttgt tatttcgctg aaagagaaca gcgattgaaa tgcgcgccgc
    62281 aggtttcagg cgctccaaag gtgcgaataa tagcacaagg ggacctggct ggttgccgga
    62341 taccgttaaa agatatgtat atgatccgcg ctataaccct ccgcgaatca acccgcccat
    62401 gccgcgacgc tccataaagg ctgcaacctg atggcgaact tcggtcgcca gttgcgcttc
    62461 cagactacgc tgcgcaagat tttctgcttc ggcataatca atgcgccgca gcaggtattt
    62521 tacgcgcgct acagaacgtc cgttcataga cagatgacga tacccaagcc caatgaggat
    62581 tgccacgcac atgggatcgc ccgccatttc accgcacaaa cggagatcga taccatgtat
    62641 ttccgcttcc cgagcgatca tcgccagagc tcgtaacatt gcaggatgaa gactgtcata
    62701 aatgttcgcc acccgggtat tgttgcgatc aacggccagg atgtattgag tcagatcgtt
    62761 ggtgccaaca gagataaaat cgacccgctt tgccagatgc ggcagcataa ataccattga
    62821 cggcacttca agcatgatgc cgatacgtgg tttgggaatt tcgtaaccga tcatctcctc
    62881 gacttcacgt ccggcacgtt caatcaggcg gcgtgcttcg tcaacttcat cgaggctggt
    62941 gaccatcggc aacagaatat tcaggttgcc cgtagcggca ttagcacgca gcatcgcccg
    63001 cacctggatc aagaagatct ccggctgatc gagcgtaatg cgaatcccac gccaacccag
    63061 gcatggattc tcttcgctga tcggcatgta aggcagctgc ttatctgctc cgacatccag
    63121 cgtacgcaag gtgacgggtt tatcattgaa catttgcagc atcccctgat actgcgccac
    63181 ctgttcttct tccgacggaa aaccactttg cagcatgaat gggatttcag tgcgataaag
    63241 accgatgcca tcaatacggc tgcccagttt ttcttcatgt tccgggctta aaccagcatt
    63301 gagcatgact tttatacgct caccgctttt taactgggcg ggtaaattga cgtcatcttc
    63361 cgccagacgg ctaagctcaa tctcttcact aattagccgc tgatattctt gcagcagtac
    63421 cggctccgga tcgaccagca attcaccgcg atagccatca acgatcagcg tcctacgatg
    63481 cagcaccgaa ggctgaatat ccgcgcccat cacggtaggg atccccagcg cacgtaccat
    63541 gatcgcagca tgggagttgg ctgcgccatc tcgcacgaca acaccgacta agcgatcctg
    63601 gggcagctca gcaagcgttg tcgctgacag ttcatctgcc accagaatga aacgttccgg
    63661 ccaggcattc ggcccttgat tagcgtcatc aagatgaaac agcaatcgct gacccagcgc
    63721 acgtaaatcg ccagcccgct ctttgagata gttatcgctt agcgcggcaa actgttcggc
    63781 aaatttttca atgaccgttt ttaccgccca ctctgccacc gagcctttat caacctcggc
    63841 aaacaattcg cgacgcagcc gggtgtccga aagcaggtgc gagtaaagat cgaaaatagc
    63901 cgccgtttct ttttgtgcac cggcggcaaa gcgtttgctg tagcggcgaa actcgtttgc
    63961 cgctccttcc agcgccccgg tcagtcgttc gcgttccaga gccggatcca gcgttgatgc
    64021 ctgatacacc tgttccatta aaggtaacgt ggcatcctgc caaccttcgg caatcgccac
    64081 accaggtgct gccggtaatg cacggattcg cgtctggcga tattgcccaa acaaggcagt
    64141 caactgcgac tgagaaagaa tagctgccat ctgggtggca agcgtcacca ggaaggattc
    64201 ttcactttcg tcatactggc gcaactctcg ttgctgtacc accagtacac caagcaactg
    64261 gcgacgttga ataattggta cgcctaaaaa cgcgcggaaa cgttcttctt ttacggaggg
    64321 gatgtatttg aagctggggt gcttttgcgc atctgcaagg tttatcggtt ccgccagcct
    64381 gccaaccagg ccgacgatcc cttcatcaaa cgcgagcgtt acagtgcgac cgcgtggttt
    64441 tttcagcccc cgggtcgcca tcaggtagta acaacgtcga tcatgatcgg caaggtaaac
    64501 cgaacagacc tcggtatcca tcgcaagaca gatgtcggta accagaatat ttaacgcctc
    64561 attcaggcgt ggtgcgctgg ctaccttttc gactatttcg cgcaggcgag tgagcataat
    64621 tggcgtgact taacctcttt tacgtcgata agcagatgcg ttttgtggtt ttggcgtatt
    64681 ttcctgcagt gacatcacca cactcgcgaa ctctttcatt accctacggt agacatcacg
    64741 tttaaatgac accacctgtc tgaccggata ccagtaactt acccatcgcc agccgtcaaa
    64801 ctctggtgta ctgctggttt gcatattgat ttctgcatcg ccgctcacca gctgcaagag
    64861 aaaccatttt tgtttttggc cgatacaaac cggcttcgtg tcccaacgca ccaaacgttt
    64921 cggtaatttg tagcgcaacc agttacgcgt tgaagcaagg attcgaacgt ctttgcggct
    64981 taatcctact tcttcaaaca attcacggta catcgcctgc tctgcggatt ctccggggtt
    65041 gattccgcct tgcggaaatt gccaggagtg ctgaccaaat cgccgggccc acattacctg
    65101 cccctggcga ttacaaatca cgatacctac gtttgggcgg tagccatcgt catcaatcac
    65161 cggactacct caaaataaag ctttatatac gaatgattgt ttcatactcc aggaagacgg
    65221 taaaccactc tctgcagggc attacacact aataacaatt gaataactca cagttatgtg
    65281 cagagttata aacagaggaa ggggtggata gccgttttta ttcacctttt ctgtggatag
    65341 agttgtgaag aactacggaa ttactacggg aaaacccgga gcattctgaa taagcccgat
    65401 atagacattt taaaatattc attatcagtc aatgcattac atgtaatttt agtaataaaa
    65461 aagagtaatt cgtgacccag gtcacacctc tcacttacgg gttggcgaaa gatcaaccaa
    65521 tgcggtattt atccacagaa tgtgccacta agttaagcac tgaaccacta aaaactggag
    65581 tttcgtcgca cgtcaaggct gtaaatggaa acagtagtgg aggtttttca cagttatccc
    65641 agctttctgt ggataacatg gtgtaagatc ctgtttattt tcagtgacca gatttggaaa
    65701 acccattgca gtgttgcgca actcgattac aggaagccta aaaagcaata taaatcagag
    65761 aattgaacaa cgcatgtgga aaaagttaca ctgcgaatat tcggcacata attgctgttt
    65821 gttttttaac caaggtatcg tgacatgtcc caacctcgcc cactgctctc tcctcccgaa
    65881 actgaagaac agttgttagc gcaagcacag caactttctg gttatacatt gggagaactg
    65941 gcggcacttg ccgggctggt tacaccagag aatttaaaac gcgataaggg ctggattggc
    66001 gtgttactgg agatctggct aggtgccagc gcaggaagta aacctgagca agattttgct
    66061 gccctgggcg tggaacttaa aactatccct gtggatagtc ttggtcgtcc gctggaaaca
    66121 acattcgttt gtgttgcccc gttaacgggt aatagcgggg tgacctggga aaccagccac
    66181 gtacgccaca agctcaaacg cgtactgtgg ataccggttg aaggcgagcg cagcatcccg
    66241 ctggcgcagc gtcgcgtagg atcaccgtta ctgtggagcc cgaatgaaga ggaagaccga
    66301 cagctacgcg aagactggga agaattaatg gatatgattg ttctcggtca ggttgagcgg
    66361 attaccgctc gtcacgggga atatttacag atacgaccga aagcagcgaa tgcgaaagca
    66421 cttaccgaag ccattggtgc ccggggcgaa cggattctga cgctgccacg cggcttttat
    66481 ttgaagaaga atttcaccag tgcactactg gcccgtcatt ttctgatcca gtagccatcg
    66541 ctttgacctg ccgctttccg ggcatataat taccgcttca tttttttggc agggcttttt
    66601 agatgttatt tgcatggata accgatccta acgcctggct tgcgctcggt acgctgacgc
    66661 tgctggagat cgttcttggg atcgacaata ttattttcct ttctctggtg gtggcaaagc
    66721 ttcccacagc acaacgtgct catgcgcgcc gtctggggtt ggcgggagcc atggttatgc
    66781 gtctggcgct gctggcatcc atcgcctggg ttacgcgcct gacgaatccg ctttttacaa
    66841 tattcagtca ggaaatttcc gcccgtgatt tgattctgct tctgggtggc ttgttcctta
    66901 tctggaaagc cagcaaggaa atccacgaat ccatcgaagg tgaagaagaa gggctgaaaa
    66961 cacgcgtttc atcattcctc ggcgctatcg tacagattat gctgctggat attatcttca
    67021 gcctcgattc agtgattacc gctgtgggtc tgtcagatca cctgtttatt atgatggcag
    67081 ccgtggtaat tgccgtaggc gtgatgatgt tcgctgcgcg ttcgattggt gattttgtcg
    67141 agcgccatcc ttcggtaaaa atgctggcgc tctctttcct gattctggtg ggctttaccc
    67201 tgattctgga aagtttcgac atccacgtac cgaaaggtta catctacttc gcgatgttct
    67261 tctctattgc ggttgaaagc ctcaacctta ttcgcaacaa aaagaacccg ctctgatact
    67321 ccgtacgctc tcctgcggga gggcgtactc actcctgttc ccctcacagt ttaagatttt
    67381 cctgctttca agatatataa cgtcggttta taaacagact attatcatag gtgggccaaa
    67441 tgaggataac cggatgaaaa aatgggcagt aataatttct gcagtcggac tggcgtttgc
    67501 tgtttccggg tgttccagtg attacgtcat ggcgaccaaa gatggccgta tgattttgac
    67561 cgatggaaaa cctgaaattg atgatgatac cgggctggtg agttatcacg atcagcaagg
    67621 taacgcgatg caaattaacc gtgatgatgt ttcgcaaatt attgaacgtt aactaataag
    67681 gtcagcatcc ggctggcctt aagatttttc tctttccctt ttcccttccc tctgccattt
    67741 ttatattcct tatgtcgtga ttataaaaag gaaacggcta tgcaatatca ccgtataccc
    67801 cacagttcgc tggaagtcag cacgctgggg cttggcacga tgacgtttgg tgaacagaac
    67861 agcgaagccg acgcccacgc acaactcgac tatgccgtcg ctcagggcat taaccttatc
    67921 gacgttgccg aaatgtaccc agtacctccg cgccccgaaa cgcaagggtt gactgaaacc
    67981 tacgtcggca actggctggc gaaacatggc agccgcgaaa agttaattat cgcctccaaa
    68041 gtgagcggac cgtcgcgcaa taatgacaag ggcatccgcc cggatcaggc gctggatcgg
    68101 aagaatatcc gcgaagcgct gcatgacagc ctcaagcgtc tgcagactga ttacctcgat
    68161 ctctatcagg tgcactggcc gcagcgccca accaactgct tcggcaaact cggttatagc
    68221 tggacggatt ctgcgcctgc ggtttcgctg ctggatacgc tggacgcact ggcagagtac
    68281 caacgcgcgg gaaaaattcg ttatatcggc gtgtcgaacg aaactgcatt tggcgtaatg
    68341 cgctacctgc atctggcgga caaacacgat ctgccgcgta ttgtcaccat tcagaacccc
    68401 tacagcctgt taaaccgcag ttttgaagta ggtctggcag aagtcagcca gtatgaaggg
    68461 gtcgaactgc tggcctattc gtgcctgggt ttcggcacac tgaccgggaa atatctcaat
    68521 ggtgcaaaac ccgctggcgc acgtaatacg ctctttagtc gcttcacccg ctatagcggt
    68581 gagcaaacgc aaaaagccgt cgcggcgtat gttgatatcg ccagacgtca tggcctggat
    68641 cctgctcaga tggcgctcgc ttttgtacgc cgtcaaccgt ttgttgccag cactctgctg
    68701 ggcgcaacca cgatggagca gctgaaaact aacgtcgaaa gtttgcatct ggagttaagc
    68761 gaagacgtat tagctgaaat tgaagcggta catcaggttt atacttatcc ggcaccataa
    68821 gattaacgcc ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg
    68881 ccgctattgc cagcgcaaac agcgcaccga agccaatgcc aatgggcacg accgggatgc
    68941 ctaccattac cgccagcgag taaatgccca gcatcaacaa catagcgctg ttttcgccaa
    69001 ggttttgtac tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca
    69061 acgcattgag cggaacgaca aaaaagcccc ccagcacgcc aatcagcatc aacaaggcat
    69121 aggctggcag cagctcatgt tgcagggaga aaatcagtac caccacgcca atcaaaatcc
    69181 cggctggcat acagcgtgat acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc
    69241 caaccacgat accaatcgct accatcgcgt tgagataggt cggcgtagcg ttatcggtaa
    69301 tgcccagcgc caccggtacc cacagcacca acaggaaacg cagcgtgaca cctgctcccc
    69361 agaataaact ggtgcccacc agcgaaaaac gcgtttcacc attgcgccat agcgaggtgc
    69421 aggcattcag gaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg
    69481 ccgccagttt gggaatgtag atattggcaa cgaccgcacc accgtaggcc agtgcgcatg
    69541 cggccagggc gaccaggaca tgccagtcag ccagcacacc accggctacg gaaccgagca
    69601 aaatcgccgc tatggtagaa gcttccatta aaccgttagc tttcactaac ttactacccg
    69661 tggttaattc gccgagaata ccgtatttcg ccggtgaata ggctgcagca ccaacaccca
    69721 ccagcgtata gccgagaaac ggattgatac caaagcagat actggctgcg cccagcagct
    69781 tcaggccgtt ggcaaacatc atcactcggc ctttggcgaa gctatccgcc acctgtccga
    69841 caaacggcgc aaaaagaatg taagcaccta caaacaccat ttgcaggatg ggctggctcc
    69901 actccggata gaactgcgct ttcagtaacg ccagagtggc aaacagcagc gcattatcgc
    69961 caaacgcaga gagaaactgc gccacgataa ccgctttcat ccccttcgac cacaacgaag
    70021 tgttagtgtg cactgactca ctcatcgtgt tgttccgctt cgtctaccca gcttttcaac
    70081 gtgacaaagt caggtttgcc gcttccaagt aatggcatct gtttcagata gcgaatatcg
    70141 cgcggtacag caagctccgg cacgccgtgc tcgcgggcat actgttgtaa cttatcgcgc
    70201 gtcagttcgt tatctgtggt gaaaagcacc agtgcctcgc ctttgctggc atcgctctta
    70261 atcgcagtgg catggacttt atctggcgaa acaccaagtg ccagttgttc caccatttcc
    70321 agcgacacca tttcgcctgc aattttggca aagcgttttg cgcggccctg aatctgcaca
    70381 aagccctgct cgtcaaaacg cacaatatcg ccagtgtcat accagccgcg ctccatttcg
    70441 ccgcgaacat tctcggcggt gggcacttcc agtacacctg gcttctccac ccgcagatag
    70501 ccgttcatta tgttcggccc tttcagttgc aggcgtccgc cctcttcgat accaggaacc
    70561 gacaacaggc gcgcgtccat acctggtaga atacgcccta ccgtaccggg tttcgccgcc
    70621 atcggtacgt tgatagaaac gacaggcgcg cactcggtca caccgtagcc ttcaaggatg
    70681 cgcaggccaa atttatcctg ccaaagctgt ttggtacttt cttgtagttt ttctgcgcct
    70741 gccaccacat agcgtagacg atagaagtca tacgggttgg cgaaacgcgc gtagtgaccg
    70801 aggaaagtcg aggtaccgaa caacacggtg caactgcggt catacaccaa ctccggcaca
    70861 atgcggtaat gcagcgggct tggataaagg aacacttctg cacctgtgag cagtggcgta
    70921 aacaggccta ccgtcagccc aaaggagtga aacagcggta acgccgacat aaagcgatcg
    70981 ttggtggtga agtcggcaat cgttttaatc tgctcgacat tcgccagaat gcttttatgg
    71041 ctatggacga cgcctttcgg atggccttca gaaccggagg taaaaaggat cagcgcctct
    71101 tcttccggct gctgttttac ctgtgccaga cgcggcatca gcaaatgaga gaagatccac
    71161 actttgtcgg cagtggtgac atctgctttt aaatcttcca gatagaccca gcgcacctga
    71221 gtaagttgct ccggcagatg ccagagtttg cctttatcga gaaactggcg ggaagtgaag
    71281 atggttttga tttcagctgc cgtaatggcg ctggtcagcc cttttacccc agcggtgtag
    71341 ttcatcattg caggaatgcg acgacgggcg atggccccaa aaatcactgc cgcactgatg
    71401 cctgcattgg gcagcattaa gccgatgcgt tcgccttcaa tactgtattt ttcaaggatg
    71461 cgtccaacaa acagcgtttt agtaagcaat ttgcgatagg agtctggggt aaagttgacg
    71521 tcttcaacac atttcttccc ggctccgaag cggtacattg cgctcagtaa agattcgtac
    71581 agcgtttcac gcgggcgcac cgccatacgc gcttccatca ttatttgatg cagcatttcg
    71641 ccagcgattt tgcgacggtc acgggcacgc ggcgcatccg gcatctccac ctgcgttggt
    71701 ggcaaaatat gcagagtaat ttgcgggaac aagcgacgtt taaccagacc tttcaggcgg
    71761 ctgaagtgcg taagttccgc cccttcaata cgcacaggaa taaccgttgc accagacttc
    71821 gccgcgacaa aacccgcacc atcgtagatt ttcatcagcg agcctgtggt ggtgattcgt
    71881 ccttcaggga aaatcaccac tggtcggccc tgttccacca gacgtaccag atgtttaatc
    71941 gccataggtt gcgtcgggtc gagaggaaca aagtcgataa atgatttcag ccaacgcata
    72001 taccactgtt ggcttattga ggtgtaaacg gcaaataccg gacgtacggg taaaaacaat
    72061 gccagcagta tgccatcaat aaaagagacg tgattaggcg tgattagaac gcgctcgccc
    72121 ttcagtgcct gggtgtcacc cgtaacgcga acgcgataca aaacacggca caaatttcga
    72181 aaaaagctaa aaagcattcc aactcccttt gctctgattc agtaaaagcg aatggaggga
    72241 gattacacga gataaagaac gcgagcgaca gtaaattagg tgcgaaaaaa aacctgcgca
    72301 tccgcgcagg ttggtgcaag agacagggta cgaagagcgt accgaataat ctcaccaatc
    72361 aatacctctg ggatcttgat tgtggtctgc acgacgactc ttcgccagtg agaaaacgca
    72421 aaggaatgaa gggaaatgca acgaggtgtg taaattgtcg gttactgtta cagattgatg
    72481 accggcaaaa aaaaacctgc gcatctgcgc aggctggtgt aattcatgtg ctcaacccga
    72541 agttgacttc acctatcaat acctctggga tcaccacttt agcaacctga agccaaacgc
    72601 caccagcggt caatcgcaac agcgtttcgc aaagtgtaac caaaggtttg caatctcttt
    72661 ttttgtctta cttatttgcg tttggctcac attcccacga tgaaaacacg ccgccccttg
    72721 aaccaacggg cgttttccgt aacactgaaa caatgtaagc gtttacccac taaggtattt
    72781 tcatggcgac cataaaggat gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc
    72841 gcgtcattaa taattcaccc aaagccagcg aagcttcccg gctggctgtg catagcgcaa
    72901 tggagtctct tagctatcac ccgaacgcca acgcccgtgc gctggcgcag cagaccactg
    72961 aaacggtcgg tctggtcgtt ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag
    73021 cggtcgaaca ggtggcttat cacactggta attttttatt gattggcaac ggttaccaca
    73081 acgaacaaaa agagcgtcag gccattgagc aactgatccg ccatcgctgt gctgcgctgg
    73141 ttgtccatgc caaaatgatc ccggatgccg atttagcctc attaatgaaa caaatgcccg
    73201 gtatggtgct gatcaaccgt atcctgcctg gctttgaaaa ccgttgtatt gctctggacg
    73261 atcgttacgg tgcctggctg gcaacgcgtc atttaattca gcaaggtcat acccgcattg
    73321 gttatctgtg ctctaaccac tctatttctg acgccgaaga tcgtctgcaa gggtattacg
    73381 atgcccttgc tgaaagtggt attgcggcca atgaccggct ggtgacattt ggcgaaccag
    73441 acgaaagcgg cggcgaacag gcaatgaccg agcttttggg acgaggaagg aatttcactg
    73501 cggtagcctg ttataacgat tcaatggcgg cgggtgcgat gggcgttctc aatgataatg
    73561 gtattgatgt accgggtgag atttcgttaa ttggctttga tgatgtgctg gtgtcacgct
    73621 atgtgcgccc gcgtctgacc accgtgcgtt acccaatcgt gacgatggcg acccaggctg
    73681 ccgaactggc tttggcgctg gcggataatc gccctctccc ggaaatcact aatgtcttta
    73741 gtccgacgct ggtacgtcgt cattcagtgt caactccgtc gctggaggca agtcatcatg
    73801 caaccagcga ctaaccgcag ttaaagcaat tccagcgcca gtaattcttc gatggtctgg
    73861 cgacggcgaa tcaaccgcgc ctgaccatta tcaaacagaa cttctggtaa cagcggacgg
    73921 ctattgtagt tggatgacat tgacgcgcca tatgcccccg tatcatgcag taccagataa
    73981 tcccctgcct tcacttccgg caaggcgcgg gtttcaacat ttcccccttc ctgctgggta
    74041 aagacatcgc ccgattcaca taatggcccg gcgacgacgg tttccaccgt tggtgcgtgt
    74101 tccagagaac gaccatcagc tgccagggca ctgatatggt ggtaactacc gtacattgcc
    74161 gggcgcatca gatcgttgaa cccggcatca accagcacaa agtggcggct acccatttgt
    74221 ttgacgctcc gcacttgagt aattaatacg ccagcctgcg ctaccagaaa gcgacccggt
    74281 tcaatttcca gtttcacagg atggcccaaa tggcgggcga tttgctcacg cgcggcattc
    74341 cacagaccat aataatgttc ggtatcaacc gcctcttcac cctgttgata aggaatagaa
    74401 agcccaccgc ctgcagaaat agcctgtaaa tcctgaccga attcgaggac ctgacgcacc
    74461 atagcaccac atacctgttc cagatgggca taatcaacgc cagaaccaat gtgcatgtga
    74521 atgccgacca gctgtagatg atgacgttgt atcacgtcca gtgcggcggg cagatcggtg
    74581 taccagatac cgtgcttgct gttttcgcca ccagtattgg ttttttggct atgcccgtga
    74641 ccaaaccccg gattaacacg cagccatacc cgatgccctg gcgaaacctg acccagttgg
    74701 tcgagcatat caacagaacc cgcattcacc ggaatttgca attcactgac gcgttcaagc
    74761 gtcgcctgat cgataacatc tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg
    74821 taacccgccg ccaacgcacg ctctatttcg cctaacgaga cggaatccac tttcacgccc
    74881 tgctcacgca ttaagcgcaa aatatgaata ttggaacagg ctttctgtgc gaagcgcacc
    74941 acatcaaact gtttcagcgc tgcaatctgc cgacgaataa tttgcgcatc gtagacccac
    75001 accgggcagc caaattctgc gggcaaacgc agcagatttt cggcggtgag atcggtatca
    75061 gtgctgaaca gtgaatgtgg cataacaaac tccagataag tgctttttta tgattacgcc
    75121 acatcataaa aagaataaaa aatatcgatt tatgttgagt ctatgcaaaa atgatatgga
    75181 ttaccggatt gcgagagagc gctaatggcc gccgttaact tacgtcatat tgaaattttt
    75241 catgcggtaa tgaccgccgg aagcctgact gaggcggcac acctgctaca cacctcacag
    75301 ccaactgtca gccgcgaact ggcgcgcttt gagaaggtga tcgggctgaa attgtttgag
    75361 cgcgtacgtg gacgattgca tcctaccgtg caaggactgc gtctgtttga agaagtgcaa
    75421 cgatcctggt acggactgga tcgcattgtc agcgccgcag aaagtctgcg cgagtttcgc
    75481 cagggagaac tgtctattgc ctgcctgccg gtcttttcgc aatctttttt accgcagctc
    75541 ctgcaaccct ttctggcacg ttatcccgat gtcagcttaa atatcgtgcc ccaggaatca
    75601 ccgctacttg aagagtggct ctcggcccag cgtcatgatt taggactcac tgagacgctc
    75661 catacgcctg cgggaacaga acgtaccgaa ttactctctt tagatgaagt gtgtgtgtta
    75721 cctccgggtc atccgctggc ggcaaaagag atattaacgc cagaagattt tcacagtgag
    75781 aactacatca gcctttcccg tactgacagc tatcgccagt tgctggatca attgtttact
    75841 gaacatcagg ttaaacgacg catgatcgta gaaacccaca gcgccgcgtc agtctgcgca
    75901 atggtacggg cgggggtagg cgtttcggta gttaacccac tcaccgcact ggattatgcg
    75961 gcaagcggtt tagtggtgcg gcggttcagc attgcggttc cattcaccgt cagcctgatc
    76021 cgccccctgc accgcccgtc atcagcgctg gtgcaggcgt ttagtgagca tttacaagcg
    76081 ggtttaccga aactggtcac ttctcttgac gcgattttgt cgtcagctac gacagcataa
    76141 aagcgacagc atcctcggca tggatcgccg cggtatcaaa cacaggcaaa acactgcgct
    76201 cttctggcac cagtaaacca atttctgtgc agccaaaaat gacgccctgt gcgccctgtt
    76261 ctgcaaggcg agcaatcact tgcgcataat aagcgcgtga cgcttcggta aattgcccca
    76321 gacacagttc ttcaaaaata atctgattaa ttttcgcccg ttcatccgct tcaggaataa
    76381 gacagttgat ggaaaattgt tccgtcagcc gcccgcgata aaaatcctgt tccatggtgt
    76441 aacgtgtacc cagcagcgcc acacgagtca ttcctgcccc ggtaattgca cgtccagtgg
    76501 catccgctat gtgtaagaaa ggcagagagc aacgagactc aatggcatcc gccactttgt
    76561 gcatcgtatt ggtacataac acaatacctt ctgcgcccgc ccgctgtaag ccaagcgccg
    76621 cctcagccag aatatccccg gttttgtccc actcaccgcg acgctggcac tcttctattt
    76681 catgaaaatc gacgctatgt agcagcactt gcgcagagtg aagcccacca agccgctgtt
    76741 taatgccttc atttatcaga cgatagtaag gaatggtgga ttcccagctc attcctccca
    76801 gcaaaccaat tgttttcatt cctctctcct gatgtgtgtt accgcaacaa ggctgaataa
    76861 ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa aaaagggccg
    76921 gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact tctcgcctgc
    76981 catcagtttg cgttcgatat gttccagggt gacatttttg gtttccggaa tgagccagaa
    77041 agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac cggcagcgcc
    77101 aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca cccagttcgt
    77161 ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt cagagcacaa
    77221 gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca tcgtcatgcc
    77281 aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca tcaggcaata
    77341 gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag ccggtttacg
    77401 ccctgcttta tctaccgtaa aaaccgcaat gaaggtggcg aacataaagg tcagccccac
    77461 gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt tgaagatacg
    77521 cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct gcaacaacat
    77581 accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc aaccgccctg
    77641 ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt ccgacgtatc
    77701 gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg ccagccagcg
    77761 cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta aagcaagaac
    77821 ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata aaaacgccag
    77881 cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc gaacgttttc
    77941 acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga cagcaatgcc
    78001 cagcaccaca cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg accctatcga
    78061 gccgagcaca aacaggatgg cccccgccat caggctgtat ttacgcccca ggcggaacga
    78121 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac tgaccaccca
    78181 ttcctgcaaa cggctggtca gcacaaagtg atcggtaatg aacggcaagg ctccggcgat
    78241 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    78301 attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    78361 aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg aacaattcac
    78421 acaggtgtca ggtcggaaat agcataaata tggattaaat tgctgcgaca tgtcgttatg
    78481 tgatggatat tccaattttc aaattaagtt gaattattga gattattatt aaccacctaa
    78541 ttttaaagca gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    78601 aaccatgata tggattatga tgatctacag gtataaaaaa ccctgccatg cggcagggtc
    78661 ataaaagtaa gaagaatgga ttaacgagcc agccaaccgc catccacggc aatggtataa
    78721 ccattcacat aatctgaagc actggaggca agaaacacta ccggccccat caggtcactc
    78781 ggcagtcccc aacggccagc tggaatacgg tcgagaattt ccgcgctacg ttgttcatct
    78841 gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    78901 atgttgtgtt tagcccattc attcgccatc aatcgcgtca cgcccatcac gccgcttttt
    78961 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    79021 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgcagcctg agacatgaag
    79081 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    79141 gcatcttcgc gccgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaactcc
    79201 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    79261 cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg ttcgacgatg
    79321 ttaatgccca caatgtcaca gcccgcttgc gccagcccca acgccatccc ctgacccagt
    79381 ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc acttaaaatc
    79441 ataacaatac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    79501 ccatatcatc aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    79561 ttccgacacc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    79621 caatatgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    79681 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    79741 acaagttacc cggagccagc tccgtcagcc ccatactcaa ttggcaggtt tccagtacat
    79801 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    79861 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcaggtgcgc
    79921 aattgtaata aaacttcgcc ggagtagcgg tatcaatact ggcaaagaca acttcttttg
    79981 cgcctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacgg
    80041 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttcc aggaaatagc
    80101 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt accggcataa
    80161 tgccgccaac aattatacgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    80221 ataccttttc aaccaaaaat tcattgcgca gcccctgggt atccagcgtt tttgcatgcg
    80281 cactgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    80341 tagaaaacga tagtgaaacg ctgtttcgtt ttctctataa ggggatgata tgcccagggg
    80401 atagggtttt caattaaaaa taaaacaatg tttcattttt taagttaagg actgaaaaaa
    80461 gtgcagtgtc gatcacgaat atgtcatgaa aatggaaaaa tcagggagcc ctgaaaatgt
    80521 atgtcgactc gccagccacc attattcaac agattgatga cggctgatat ttatcggtaa
    80581 ggaggaaatg cagagctatt cgtcacgttc aatggtcaat gccacgccct ggcccccgcc
    80641 gatacaaagc gtagccagtc ctttgcgggc attacgtttc accatttcat gaaccagaga
    80701 aacgaggatt cggcaaccgg aagcgcctat tgggtgaccg agtgcgatcg cgccaccatt
    80761 gacattgacc cgacgctcat cccattcaag catcttgcca accgaaagcg cctgtgcagc
    80821 aaacgcttca ttagcctcga taagatcgac atcagccaac tgccagccaa cacgctccag
    80881 gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat gccggatcta caccaacgct
    80941 ggcaaatgcg cggatgcggg ccagcacggg taaattcaac gctcgtgctt tggcttcgct
    81001 catcatcatt actgcagctg cgccatcgtt tatggatgat gcattacccg ctgtcacaga
    81061 gccgagacta tcaaatgaag gatttaaacg ggctaagcct tctgcactgg cgtcagtgcg
    81121 tggctgttca tcggtatcta caaccaacgt ctgaccgtta ctttgggtca ttaccgggac
    81181 gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag
    81241 tgcgtaagca tcctgcaact gacggctgat gccatattcg cgagccagat tttcggcggt
    81301 gacaccaata tgataatcat tgaaggcatc ccacaaccca tcgtgcacaa gactatcaac
    81361 caactggcta ttgccaagct gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg
    81421 actcatgttt tcctgaccac cggcgatgac aatatcagcc tcgccacact gtatcgcctg
    81481 agtagccaga tgcagtgctt taagcccgga accgcaaacg tcattaatcg tgattgcaga
    81541 aacgctgtta ggaagaccac ctttaatagc cgattgcctt gccggattct gccctgcccc
    81601 tgcagtcaac acctgaccaa gaatcacttc atccaccgca tattcagaaa caccggtacg
    81661 ttctattaat gctttcacga ccaggctgcc aagttccacg gcggaatgac ccgctaacgc
    81721 accacgaaag cagccgatag gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac
    81781 aacctcgcac caattaacag tgcaatagta aatgattgtt aattacagtt atcttaattg
    81841 tttaaaaaaa agtgattttt atcacaaagg gaatacgcct gaccagcagt cagagacata
    81901 actggcacgt aaggtttgca accactaacc caccaataga gaggtagaaa gggcgttaat
    81961 ctcccatact taacctggtt tatggtaaat tgccctccaa tttgtttaat ttgtagatga
    82021 tacgttcaga taatgtctaa tatttggtca aaagaagaaa ctctgtggag tttcgcgctc
    82081 tacggcacag ccgttggtgc aggcacgctc ttccttccta ttcagttagg ttcggcaggg
    82141 gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatattg gccacataaa
    82201 gccttatgcc agttcatcct ctcatcaaaa acatcagcag gtgaagggat aacgggcgcg
    82261 gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta cttcatcgcc
    82321 ttttttgtcg tcgtgttgat atatgccgtg gcaattacca actcacttac ggaacagctg
    82381 gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta
    82441 atcctgaatc tcatttttct gatgggacgc catgccacta ttcgggtaat gggatttttg
    82501 gtattcccat tgattgccta tttcttattt ctttctattt acctggtcgg tagttggcaa
    82561 cctgatctat taactaccca gatagagttc aatcagaata cccttcacca gatatggata
    82621 tcgattcccg tgatggtttt cgcctttagc catacgccca ttatttctac gtttgccata
    82681 gacagacgtg aaaaatatgg cgaacatgct atggataaat gcaaaaaaat tatgaaagtc
    82741 gcttatctca tcatctgcat aagtgtactg ttctttgtct ttagctgcct gctttctatt
    82801 ccaccttcgt atattgaagc ggctaaagaa gaaggggtta ccattttatc ggcgctttct
    82861 atgctgccga acgccccagc atggttgtca atttccggga ttattgtcgc agtagttgcg
    82921 atgtcgaaat cattcctggg tacgtacttt ggcgttattg aaggtgccac agaggtcgtc
    82981 aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg catttaaccg cgcactatca
    83041 attatgttgg tatcgctgat taccttcatt gtttgttgca ttaacccgaa cgcgatttcg
    83101 atgatttacg cgatcagcgg cccgctcatt gccatgatac ttttcatcat gcctacgctg
    83161 tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca tcggaaatct gattacgctg
    83221 atcgtgggta tcttgtgcgt atcggtaatg ttctttagct aatatccatt gtgaatggtt
    83281 caaaattgct acaattttga accattccag ttttcataat gttcatttgt tataattccg
    83341 ctctatcact tctctcgt
//
LOCUS       DAYPHN010000003        76505 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000003 DAYPHN010000000
VERSION     DAYPHN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.003"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..230
                     /gene="zapD"
                     /locus_tag="ACG0BW_000166"
     CDS             <1..230
                     /gene="zapD"
                     /locus_tag="ACG0BW_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFK7257184.1"
                     /translation="DLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQIS
                     GHKSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            240..437
                     /gene="yacG"
                     /locus_tag="ACG0BW_000167"
     CDS             240..437
                     /gene="yacG"
                     /locus_tag="ACG0BW_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFK7257185.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(525..923)
                     /gene="mutT"
                     /locus_tag="ACG0BW_000168"
     CDS             complement(525..923)
                     /gene="mutT"
                     /locus_tag="ACG0BW_000168"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFK7257186.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMSE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLIADDFPPANEPVIAKLKRVYVG"
     gene            complement(983..3688)
                     /gene="secA"
                     /locus_tag="ACG0BW_000169"
     CDS             complement(983..3688)
                     /gene="secA"
                     /locus_tag="ACG0BW_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFK7257187.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3750..4262)
                     /gene="secM"
                     /locus_tag="ACG0BW_000170"
     CDS             complement(3750..4262)
                     /gene="secM"
                     /locus_tag="ACG0BW_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFK7257188.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(4493..5410)
                     /gene="lpxC"
                     /locus_tag="ACG0BW_000171"
     CDS             complement(4493..5410)
                     /gene="lpxC"
                     /locus_tag="ACG0BW_000171"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFK7257189.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5511..6662)
                     /gene="ftsZ"
                     /locus_tag="ACG0BW_000172"
     CDS             complement(5511..6662)
                     /gene="ftsZ"
                     /locus_tag="ACG0BW_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFK7257190.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6723..7985)
                     /gene="ftsA"
                     /locus_tag="ACG0BW_000173"
     CDS             complement(6723..7985)
                     /gene="ftsA"
                     /locus_tag="ACG0BW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFK7257191.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7982..8812)
                     /gene="ftsQ"
                     /locus_tag="ACG0BW_000174"
     CDS             complement(7982..8812)
                     /gene="ftsQ"
                     /locus_tag="ACG0BW_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFK7257192.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(8814..9734)
                     /gene="ddlB"
                     /locus_tag="ACG0BW_000175"
     CDS             complement(8814..9734)
                     /gene="ddlB"
                     /locus_tag="ACG0BW_000175"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFK7257193.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9727..11202)
                     /gene="murC"
                     /locus_tag="ACG0BW_000176"
     CDS             complement(9727..11202)
                     /gene="murC"
                     /locus_tag="ACG0BW_000176"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFK7257194.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEAQHD"
     gene            complement(11256..12323)
                     /gene="murG"
                     /locus_tag="ACG0BW_000177"
     CDS             complement(11256..12323)
                     /gene="murG"
                     /locus_tag="ACG0BW_000177"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFK7257195.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(12320..13564)
                     /gene="ftsW"
                     /locus_tag="ACG0BW_000178"
     CDS             complement(12320..13564)
                     /gene="ftsW"
                     /locus_tag="ACG0BW_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFK7257196.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13564..14880)
                     /gene="murD"
                     /locus_tag="ACG0BW_000179"
     CDS             complement(13564..14880)
                     /gene="murD"
                     /locus_tag="ACG0BW_000179"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFK7257197.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14883..15965)
                     /gene="mraY"
                     /locus_tag="ACG0BW_000180"
     CDS             complement(14883..15965)
                     /gene="mraY"
                     /locus_tag="ACG0BW_000180"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFK7257198.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15959..17317)
                     /gene="murF"
                     /locus_tag="ACG0BW_000181"
     CDS             complement(15959..17317)
                     /gene="murF"
                     /locus_tag="ACG0BW_000181"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFK7257199.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDFAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALIARIKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(17314..18801)
                     /gene="murE"
                     /locus_tag="ACG0BW_000182"
     CDS             complement(17314..18801)
                     /gene="murE"
                     /locus_tag="ACG0BW_000182"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFK7257200.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLSDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLSDLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18788..20554)
                     /gene="ftsI"
                     /locus_tag="ACG0BW_000183"
     CDS             complement(18788..20554)
                     /gene="ftsI"
                     /locus_tag="ACG0BW_000183"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFK7257201.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20570..20935)
                     /gene="ftsL"
                     /locus_tag="ACG0BW_000184"
     CDS             complement(20570..20935)
                     /gene="ftsL"
                     /locus_tag="ACG0BW_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFK7257202.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20932..21873)
                     /gene="rsmH"
                     /locus_tag="ACG0BW_000185"
     CDS             complement(20932..21873)
                     /gene="rsmH"
                     /locus_tag="ACG0BW_000185"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFK7257203.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21875..22333)
                     /gene="mraZ"
                     /locus_tag="ACG0BW_000186"
     CDS             complement(21875..22333)
                     /gene="mraZ"
                     /locus_tag="ACG0BW_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFK7257204.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22935..23939)
                     /gene="cra"
                     /locus_tag="ACG0BW_000187"
     CDS             complement(22935..23939)
                     /gene="cra"
                     /locus_tag="ACG0BW_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFK7257205.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(24021..24107)
                     /locus_tag="ACG0BW_000188"
     CDS             complement(24021..24107)
                     /locus_tag="ACG0BW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFK7257206.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(24119..24610)
                     /gene="ilvN"
                     /locus_tag="ACG0BW_000189"
     CDS             complement(24119..24610)
                     /gene="ilvN"
                     /locus_tag="ACG0BW_000189"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFK7257207.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24613..26337)
                     /gene="ilvI"
                     /locus_tag="ACG0BW_000190"
     CDS             complement(24613..26337)
                     /gene="ilvI"
                     /locus_tag="ACG0BW_000190"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFK7257208.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYIWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQIAHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            26456..26500
                     /locus_tag="ACG0BW_000191"
     CDS             26456..26500
                     /locus_tag="ACG0BW_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257209.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(26655..27599)
                     /gene="leuO"
                     /locus_tag="ACG0BW_000192"
     CDS             complement(26655..27599)
                     /gene="leuO"
                     /locus_tag="ACG0BW_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFK7257210.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            28258..28344
                     /gene="leuL"
                     /locus_tag="ACG0BW_000193"
     CDS             28258..28344
                     /gene="leuL"
                     /locus_tag="ACG0BW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFK7257211.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            28437..30008
                     /gene="leuA"
                     /locus_tag="ACG0BW_000194"
     CDS             28437..30008
                     /gene="leuA"
                     /locus_tag="ACG0BW_000194"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFK7257212.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            30008..31099
                     /gene="leuB"
                     /locus_tag="ACG0BW_000195"
     CDS             30008..31099
                     /gene="leuB"
                     /locus_tag="ACG0BW_000195"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFK7257213.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVAEGV"
     gene            31102..32502
                     /gene="leuC"
                     /locus_tag="ACG0BW_000196"
     CDS             31102..32502
                     /gene="leuC"
                     /locus_tag="ACG0BW_000196"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFK7257214.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLRAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            32513..33118
                     /gene="leuD"
                     /locus_tag="ACG0BW_000197"
     CDS             32513..33118
                     /gene="leuD"
                     /locus_tag="ACG0BW_000197"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFK7257215.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(33226..33357)
                     /gene="sgrT"
                     /locus_tag="ACG0BW_000198"
     CDS             complement(33226..33357)
                     /gene="sgrT"
                     /locus_tag="ACG0BW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFK7257216.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            33446..35104
                     /gene="sgrR"
                     /locus_tag="ACG0BW_000199"
     CDS             33446..35104
                     /gene="sgrR"
                     /locus_tag="ACG0BW_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFK7257217.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(35601..35894)
                     /locus_tag="ACG0BW_000200"
     CDS             complement(35601..35894)
                     /locus_tag="ACG0BW_000200"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7257218.1"
                     /translation="MDKISLCSSNLLGADLHGAKMNNAKVISSDLSDTNLSDTDMSDT
                     DLDDTLLRNAKMDNTILNNAYMEDTNVRGINLSKADLSGQDSAKLRSRGAIID"
     gene            complement(35874..36437)
                     /locus_tag="ACG0BW_000201"
                     /pseudo
     CDS             complement(35874..36437)
                     /locus_tag="ACG0BW_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     regulatory      36678..36777
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            36808..37791
                     /gene="thiB"
                     /locus_tag="ACG0BW_000202"
     CDS             36808..37791
                     /gene="thiB"
                     /locus_tag="ACG0BW_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFK7257219.1"
                     /translation="MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYATANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            37767..39377
                     /gene="thiP"
                     /locus_tag="ACG0BW_000203"
     CDS             37767..39377
                     /gene="thiP"
                     /locus_tag="ACG0BW_000203"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFK7257220.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPTRAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALVAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            39361..40059
                     /gene="thiQ"
                     /locus_tag="ACG0BW_000204"
     CDS             39361..40059
                     /gene="thiQ"
                     /locus_tag="ACG0BW_000204"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFK7257221.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTSTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASALLGITG"
     gene            complement(40173..40937)
                     /gene="yabI"
                     /locus_tag="ACG0BW_000205"
     CDS             complement(40173..40937)
                     /gene="yabI"
                     /locus_tag="ACG0BW_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFK7257222.1"
                     /translation="MQALLEHFITQSTVYSLIAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAVGVVALVVLIRHPLMPMYIDILRKVVGG"
     gene            complement(41023..41901)
                     /gene="araC"
                     /locus_tag="ACG0BW_000206"
     CDS             complement(41023..41901)
                     /gene="araC"
                     /locus_tag="ACG0BW_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFK7257223.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            42240..43940
                     /gene="araB"
                     /locus_tag="ACG0BW_000207"
     CDS             42240..43940
                     /gene="araB"
                     /locus_tag="ACG0BW_000207"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFK7257224.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            43951..45453
                     /gene="araA"
                     /locus_tag="ACG0BW_000208"
     CDS             43951..45453
                     /gene="araA"
                     /locus_tag="ACG0BW_000208"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFK7257225.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSINTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            45738..46433
                     /gene="araD"
                     /locus_tag="ACG0BW_000209"
     CDS             45738..46433
                     /gene="araD"
                     /locus_tag="ACG0BW_000209"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFK7257226.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            46508..48859
                     /gene="polB"
                     /locus_tag="ACG0BW_000210"
     CDS             46508..48859
                     /gene="polB"
                     /locus_tag="ACG0BW_000210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFK7257227.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADHVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLINYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDTSALDFKLEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERSRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            49023..51929
                     /gene="rapA"
                     /locus_tag="ACG0BW_000211"
     CDS             49023..51929
                     /gene="rapA"
                     /locus_tag="ACG0BW_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFK7257228.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            51941..52600
                     /gene="rluA"
                     /locus_tag="ACG0BW_000212"
     CDS             51941..52600
                     /gene="rluA"
                     /locus_tag="ACG0BW_000212"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFK7257229.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            52813..52991
                     /locus_tag="ACG0BW_000213"
                     /pseudo
     CDS             52813..52991
                     /locus_tag="ACG0BW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(53008..53739)
                     /locus_tag="ACG0BW_000214"
     CDS             complement(53008..53739)
                     /locus_tag="ACG0BW_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7257230.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKIDVKMNIFQRLWETLR
                     HLFWSDKQPEAYTLLFNFVNNKVGNINASEYFTGAIDENEREKFINSLELFNKLKTCA
                     KNPDEMVAKANMCWDTQIYGDIELSVTFLIEKNEICTQTLKLCKGLGSLGVDLRKAYL
                     PGVDMRNCYLGPNIMKGNDKSLYLEPGWNANLDGANLEGAILDGTDAIIINKITTKKI
                     ATEEITTKQSTAEKLLPTQLLNDPE"
     gene            complement(54034..54849)
                     /gene="djlA"
                     /locus_tag="ACG0BW_000215"
     CDS             complement(54034..54849)
                     /gene="djlA"
                     /locus_tag="ACG0BW_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFK7257231.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            55104..57458
                     /gene="lptD"
                     /locus_tag="ACG0BW_000216"
     CDS             55104..57458
                     /gene="lptD"
                     /locus_tag="ACG0BW_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFK7257232.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            57511..58797
                     /gene="surA"
                     /locus_tag="ACG0BW_000217"
     CDS             57511..58797
                     /gene="surA"
                     /locus_tag="ACG0BW_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFK7257233.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            58797..59786
                     /gene="pdxA"
                     /locus_tag="ACG0BW_000218"
     CDS             58797..59786
                     /gene="pdxA"
                     /locus_tag="ACG0BW_000218"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFK7257234.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     NRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            59783..60604
                     /gene="rsmA"
                     /locus_tag="ACG0BW_000219"
     CDS             59783..60604
                     /gene="rsmA"
                     /locus_tag="ACG0BW_000219"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFK7257235.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            60607..60984
                     /gene="apaG"
                     /locus_tag="ACG0BW_000220"
     CDS             60607..60984
                     /gene="apaG"
                     /locus_tag="ACG0BW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFK7257236.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            60991..61833
                     /gene="apaH"
                     /locus_tag="ACG0BW_000221"
     CDS             60991..61833
                     /gene="apaH"
                     /locus_tag="ACG0BW_000221"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFK7257237.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLKTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(61911..62390)
                     /gene="folA"
                     /locus_tag="ACG0BW_000222"
     CDS             complement(61911..62390)
                     /gene="folA"
                     /locus_tag="ACG0BW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFK7257238.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(62582..64444)
                     /gene="kefC"
                     /locus_tag="ACG0BW_000223"
     CDS             complement(62582..64444)
                     /gene="kefC"
                     /locus_tag="ACG0BW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFK7257239.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMMTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(64437..64967)
                     /gene="kefF"
                     /locus_tag="ACG0BW_000224"
     CDS             complement(64437..64967)
                     /gene="kefF"
                     /locus_tag="ACG0BW_000224"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFK7257240.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(65075..66406)
                     /gene="yaaU"
                     /locus_tag="ACG0BW_000225"
     CDS             complement(65075..66406)
                     /gene="yaaU"
                     /locus_tag="ACG0BW_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7257241.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(66465..66752)
                     /gene="fixX"
                     /locus_tag="ACG0BW_000226"
     CDS             complement(66465..66752)
                     /gene="fixX"
                     /locus_tag="ACG0BW_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFK7257242.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(66749..68035)
                     /gene="fixC"
                     /locus_tag="ACG0BW_000227"
     CDS             complement(66749..68035)
                     /gene="fixC"
                     /locus_tag="ACG0BW_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFK7257243.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSVPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(68086..69027)
                     /gene="fixB"
                     /locus_tag="ACG0BW_000228"
     CDS             complement(68086..69027)
                     /gene="fixB"
                     /locus_tag="ACG0BW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFK7257244.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKDNIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(69042..69812)
                     /gene="fixA"
                     /locus_tag="ACG0BW_000229"
     CDS             complement(69042..69812)
                     /gene="fixA"
                     /locus_tag="ACG0BW_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFK7257245.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFERALPQQTAT
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            70285..71799
                     /gene="caiT"
                     /locus_tag="ACG0BW_000230"
     CDS             70285..71799
                     /gene="caiT"
                     /locus_tag="ACG0BW_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFK7257246.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            71830..72972
                     /gene="caiA"
                     /locus_tag="ACG0BW_000231"
     CDS             71830..72972
                     /gene="caiA"
                     /locus_tag="ACG0BW_000231"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFK7257247.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            73101..74318
                     /gene="caiB"
                     /locus_tag="ACG0BW_000232"
     CDS             73101..74318
                     /gene="caiB"
                     /locus_tag="ACG0BW_000232"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFK7257248.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNVMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            74392..75945
                     /gene="caiC"
                     /locus_tag="ACG0BW_000233"
     CDS             74392..75945
                     /gene="caiC"
                     /locus_tag="ACG0BW_000233"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFK7257249.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLIDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            76054..>76505
                     /locus_tag="ACG0BW_000234"
     CDS             76054..>76505
                     /locus_tag="ACG0BW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFK7257250.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILP"
BASE COUNT        18412 a        20935 c        19328 g        17830 t
ORIGIN      
        1 tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat ggtttttatc
       61 aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat tcacagcttt
      121 atccgcaaat ttccggtcat aagagccgtt ttgccattcg ttttatgccg ctggacagtg
      181 aaaacggaca ggtaccggaa cgtctggatt tcgaattggc ctgttgctaa ggagtaaaaa
      241 tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggta tggggtgaaa
      301 tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc ggagaatggg
      361 ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat gactggagcg
      421 aagagccaaa gcagtgacat tttgtctgat gcgacgctgg cctgataaga cgcgccagcg
      481 tcgcatcggg catcttgtgc aaatgttgga tgcggcataa acacctatcc gacatacaca
      541 cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc aatcagaccg
      601 accagcgaca tccactcacc gggttgccct tctttacccc acggctcccc ttcccagctt
      661 tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatattc cagtttttca
      721 aatagcgaaa aatgttgtgg ggtaatcccg acctcttcct gaagttcacg caccaccgcc
      781 tgttccggcg tttcgctcat ttcaatttta ccgccgggaa actccagttt attcgccatg
      841 tgcgcatctg ctgcgcgacg ggttataaag atttcattgt tctcgttgcg aataatacct
      901 accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag aatcctgcgc
      961 cttttacttc aactgttagc ttttattgca ggcgaccatg gcactgcttg tattttttac
     1021 cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ttcaccggtt tgcgccgcca
     1081 gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt tgcgctaaac
     1141 gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc atacgtacct
     1201 gaactttgct cagcgtgctg ataacttcat atttcaacga ctccagcatc gctgcaaaca
     1261 tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag ccacgcaggt
     1321 ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacaga gagtcgagag
     1381 tttgcagcat gacgcctttt tcgaagtgac gcatcatctc agcaccaacc acttcttctt
     1441 tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc tcttcatgca
     1501 gttctggttc tttatccagc cactcggcaa ttggcaaatc gaggtcgaaa tcgttcttca
     1561 gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc ggaatgtagg
     1621 catcaatggt cgctttgaac acatcttcac gaatgctgtt gatggtttcg ctcacatcgc
     1681 tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg ttagccacgt
     1741 catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact ttacgctggg
     1801 cattggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc ttcataccca
     1861 gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc gcatcttcca
     1921 tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga ccgcgcaact
     1981 ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg ccacctgctg
     2041 ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt tgctctgcgg
     2101 tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca atatctgtac
     2161 cacgacccgc catgttggtc gcgatagtca ccgcagccgg ataacctgcc tgagcaacaa
     2221 tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt gtgcttaata ccggctttgg
     2281 tcagttcgtt tgataccagc tccgattttt cgatggagat agtacccacc agcaccggct
     2341 ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt tccgcttcag
     2401 tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc ggaacaacga
     2461 cggtatccag cttgtagatg gagctgaatt cgaaagcttc ggtatcagca gtaccggtca
     2521 tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgac gccagcgtct
     2581 ggttttcgtt ctggatctgt acaccttctt tcgcttccac tgcctgatgc aggccatcgg
     2641 accaacgacg tccctgcatg gtacgaccgg tgtgttcgtc aacgatgata acttcaccat
     2701 ctttaacgat gtagtcgacg tctcgggtaa acagcgcatg agcgcgcagc gccgccgtta
     2761 cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca tccatgatgc
     2821 cttctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg ttcacctggc
     2881 gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag tcttcttttt
     2941 cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc gagctgtctt
     3001 ctgccgggcc ggaaatgatc agcggtgtac gagcttcatc gatcaggatg gagtccactt
     3061 cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg aacgccatgt
     3121 tatcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg tcagctgcgt
     3181 aagcttcacg cttcgccggt gctggcatgc ccggcaggtt gataccgaca gtcaggccaa
     3241 ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag tcgttgacgg
     3301 taacaacgtg tacgccctta ccggttaatg cgttcaggta agcaggcagc gttgcggtca
     3361 gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga accataccgc
     3421 cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc tcacgcacca
     3481 cggcgaacgc ttccgggatc agattttcca gcacttcgcc tttttccaga cgcgcacgaa
     3541 actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc ggttccatgg
     3601 cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta cgactaccga
     3661 aaactttagt taataatttg attagcataa taaaatctca aacgccccgc gttgcggagt
     3721 caataaaatg aagtaaaggt ttattgttgt tagctgaggc gttgagggcc agcacggatg
     3781 ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgcggggt aaaatgcgca
     3841 taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcaacag cgcgctgagc
     3901 gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc aacgggcagc
     3961 gtttgcggtg ccattgcgaa ggaaagatga cggattaccg tgcgaatggc atgttgatgc
     4021 cagtaatcaa cggaatagtt cgaattcggg cggcgcgtgt tcgcttccag caaggccaat
     4081 tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt tgcgggcgcg
     4141 tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac catccctaat
     4201 aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag tattccactc
     4261 acgttattgc catcccgatc gtattcaaac gtatttagcg cacaaatctt atcattaaag
     4321 cctgaagtcc acagcacgaa tgaagataag ttgtgcgtaa aagtgcttaa gaaaacagcg
     4381 ttcgcaccgt tttttatcgt tcttataaaa ttcaaaaaaa aacgactggt tcacctggcc
     4441 ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt cgttatgcca
     4501 gtacggctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg aaggtcacat
     4561 attcccaggc ttcctgtttt gcaaggacag cctgcagcag tttgttattc agtgcatgac
     4621 cggatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac aagtcaccga
     4681 tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg tcttcgttca
     4741 gtacgcgata atcgtcaaca acgatggcac aatcgaagct accgcccagg cacaaaccac
     4801 gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg ctgatctggc
     4861 gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa tcaatagccg
     4921 gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta aattcagccc
     4981 acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttc ttggcgcagt
     5041 tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg ctgccgtcca
     5101 tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc aggcccgcga
     5161 gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg accagacacg
     5221 tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc ggtggattca
     5281 agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac gtcagggtga
     5341 ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta agtgtccttt
     5401 gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac tatacattcg
     5461 gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc ttaatcagct
     5521 tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt ttgcggtgca
     5581 ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc catcccgtgc
     5641 tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag agtgatttca
     5701 ggacgtttgt ccatgccgat acctgtcgca acaacagtta cgcgcagctc gtcattcata
     5761 tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa tgcacggatg
     5821 gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt gatgttaacc
     5881 agcacgccgc gtgcgccaga caggtcgata tcttccagca gcggagaaga gatagccatt
     5941 tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat cattgcgtag
     6001 cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa acccggacga
     6061 gtaatcagtt cggcgatacc ttgcacagcg cctttcagta catcgttcgc tgcgccaaac
     6121 gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt cgggatagtg
     6181 atcagagagt ccacatgctt ggacagttca gtgatcccct gctcggcgaa tgccatacgc
     6241 ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat acccaaatct
     6301 tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccccc catacccgca
     6361 gcaataaaga ccatgtctgc accttccagc gccgcacgca gtgcatcgcg atcctcatca
     6421 gccgcattgc ggccaacttc tggattagcg cctgcgccca gtcctttggt gataccgcta
     6481 ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc ggtatttacc
     6541 gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc attaccgccg
     6601 ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat tggttcaaac
     6661 atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg gcctcataaa
     6721 aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc aactgatgct
     6781 gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actcttttcc atagtgaagc
     6841 aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc ggtaatgttc
     6901 agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc tgcaagacct
     6961 tcgatctgcg ctgcgccacc agttaataca atgcctgccg ccaggtgatg tttaacccct
     7021 tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt gagcagctcg
     7081 gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg cggcggacga
     7141 ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc cagcgcacaa
     7201 ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc gtaagcgata
     7261 tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa tgccccaccg
     7321 gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac acccagttca
     7381 cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat cagttggtca
     7441 actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc gttgtgacat
     7501 gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg attcttgatc
     7561 ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg atgctcatcg
     7621 cgcacacgca ccgatttcgc ggtatggacg acattttcca catcttcttg cgtcacttct
     7681 tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt accagaaagc
     7741 gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc aatggcgcgt
     7801 tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat accacgcgac
     7861 gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc ccctactaaa
     7921 gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc cgtcgccttg
     7981 atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct ggcggcaagg
     8041 gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta atccgtttgc
     8101 catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga gccaaacgtt
     8161 tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc aactgccagg
     8221 aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc agcatctgcc
     8281 ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca tacagcattg
     8341 gaagcacctg cttgctggtg cgatctggcg gcacgctgaa agtgtttcct tccgcgtcta
     8401 ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga atcttcaatt
     8461 catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt tgttctattt
     8521 gcgtctggat gatgttgaca tcctgggtca taaaggtacc tggctcaccc aatgccagga
     8581 tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac accagctttg
     8641 agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg ctcaccaaca
     8701 ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca ttattgcggc
     8761 gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac atattagtcc
     8821 gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg acgtgccgcc
     8881 atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag cagataaaac
     8941 tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc taacgtcgtc
     9001 catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag acctgcgggg
     9061 cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa ggttccggac
     9121 ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt gaactccggc
     9181 ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa tcttaatgca
     9241 tcttgtagag cattttctgc tactactttt gacattccca cactggaacc ttcgcggctc
     9301 ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt atcactcagg
     9361 cctttttcaa actctgcgcg ggttaacgct acccacggag cgaccggtaa accggcacct
     9421 tgccacagaa gtttgctgcg tagtttatcc attgaaagcg cagatgccat cactccgctg
     9481 ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtgccatc ttcaccgccg
     9541 cgaccgtgta gcgcgataaa cactttctga aagcccatcg acttcagttg cgtcacgtcg
     9601 acttctttcg ggtcgacagg atacgcgtca ataccgcctt cacgcagccc ggctaacacc
     9661 gctgcgccag aattcagaga aacttcccgc tcagcggagg tcccacccaa caggaccgcg
     9721 attttatcag tcatgttgtg cttcctccgg agtttgcggc ttcagtttga tttcagctaa
     9781 agaacgggca atttttccaa tattaccagc cccctgaacg agaatcaggt cgttaccggt
     9841 taataccggt gccagcatct cggctacctg cgccgggtca ggcaccagaa tgggatcaat
     9901 tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg gaattggcgc
     9961 ttcgccagcc ggatacactt ccagcattaa cagggtatca acctgcgtca gcacattggc
    10021 gaaatcatca tacaggtcgc gtgtacgggt aaaacggtgc ggctgaaaca gcattaccag
    10081 gtttttatcc ggccagcctg cacgcgccgc tttaatagtg gcgtccactt ccgtcgggtg
    10141 gtggccgtag tcatcgacca acatcgccgt accgctttta ccattcactg gctccagcgg
    10201 gaattcaccg aggaaatcaa aacggcgacc agtcccctgg aagctctcca gcgcacgcaa
    10261 aatagcctcg tcgtcaatgc cctcttccgt agcaaccgca accgcagctg cggcgttcag
    10321 cgcgttatga cgacctggcg cattcagggt gacgcgcatc ggctctttgt cctggcgcag
    10381 cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca cgtcggcatc
    10441 ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaacaatt cgcggatcac
    10501 cggatcatca acacacatca ctgcacgacc gtaaaacggc aggttgtgca gaaaattaat
    10561 aaaggtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt cggcttcgat
    10621 attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac tctcatctgc
    10681 ttcggcaatc aggtaccgac catgccccaa acgcgcatga acgcccgccg cttttaccag
    10741 cccaccgtta acgaaggttg ggtcgagtcc cgcttctgcg tagatgctgg aaaccatcgc
    10801 agtggttgtc gttttgccgt gcgttccggc aatggcgatg ccatgacgaa aacgcattaa
    10861 ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag ctgcgacaat
    10921 ttccgggtta tcggcagaaa tcgcgctgga aacaacgacc acgctggcat cacgtacgtt
    10981 ttccgggcga tggttgaaat aaatcgtcgc cccaagattc attaactgct gcgtgaccgg
    11041 atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa cttcggcaat
    11101 accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc gacgcatttc
    11161 gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg ccattaactt
    11221 cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc ccggctcact
    11281 tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg ttctgccatg
    11341 gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc cacgctaagc
    11401 tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagagc attccagtat
    11461 tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc cgcagcgatt
    11521 tcactcaccg ttaacgcacc ggagcggcaa acgacgacat ccgcccacgc atacgccgcc
    11581 gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc ggcatacgcc
    11641 tgttcaacgg attgttgcga acctttaccg ctctgatgcc agatagtgac tgaatcaccc
    11701 agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg ggaaccaccc
    11761 actaccaaca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg caacggcagc
    11821 gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa agcgcctgga
    11881 aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa gcccgcaata
    11941 ccgttttgtt catgaagcac aaccggaatg cctaacgacc acgcggccag accacctgga
    12001 cctgatacgt agccgcccat tccgagcacc acgtcaggtt tgtacgcttt cataatcgcc
    12061 cgcgcctgac gccaggcgtt gaagattcgc agtggcgcag ctatcagtgc ttttatacct
    12121 tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt tggcactaag
    12181 tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg agccattaga
    12241 tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc catcaccatt
    12301 aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgtgct ttctccagac
    12361 gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc agtaagctcg
    12421 aaccaccgta actgatcaac ggcaatgtca gacctttggt cggtaacatc cccgcggccg
    12481 cgcctacgtt cactagcgcc tggaagctaa accagatgcc aatggaacag gcgagaaaac
    12541 cggaaaaacg atggtcaatt tctaatgctt tacggccaat cgacatcgca cgaaaagcga
    12601 cgaagaatac cattaaaagt gctagtacca caccgacata acctaattct tcgccgataa
    12661 tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt acggagttac
    12721 ctaagccttg cccccacagt tcgccgcgac caaacgccat cagcgattgc gttaactgat
    12781 agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg cggatacggt
    12841 acggttcggc gaggatcagc aacacaaccg ctgaaatgcc cataccgata atggcaatga
    12901 actgccacaa tttcgctccc gccaggaata acatcgccag cgtagtcaca aacaacacca
    12961 ccaccgtacc aaggtctggc tgtgccagca gtaatactgc taacaccaga atcacgccca
    13021 tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc accagatagt
    13081 tggcgatata gcaaaacaac gacagttttg tcagctccgc aggctggata cgcagcaagc
    13141 cgagatcgat ccaacgcgat gcccctttaa cagagctacc cactaccaga acgatcatca
    13201 acaggatgat tgatccgagt agcatcgtag cgctgtaacg ttgccagaac tccatcggca
    13261 gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca tcacgcttcg
    13321 cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc gaggtcacca
    13381 tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga tcgtacatga
    13441 tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc gccgtggaga
    13501 tccagaccag aatactgaat cctggcaggc gcggcatttt caggcgaggg agagataaac
    13561 gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt caaagttctt
    13621 gaactgatca aggctggcac atgccgggga gagcagaacc atatcgcccg gctgaacacg
    13681 cggagccagc aagcgcatcg cctgttccat ggtttcggtt tgttctgcca cttccgggcg
    13741 tagcgccgcc agctgcgcgc cgtcccgacc gaaacaatac agacgtacgt tatcgccatt
    13801 caggtaacgc gccagtgggc taaaatctgc cgatttacca tcgccgccca gcaacaaatg
    13861 cagcgtgccg tctacgtgca ggccattcag tgccgcttcc gtactgccga cgttggtcgc
    13921 ttttgaatcg ttaatccagc gcacaccgtt atgctccagc acaacttcaa agcgatgcgg
    13981 cagaccagtg aatgtggtta acgctttcag gctgctggcg cgcggcaaac cagcggcatc
    14041 tgccagtgcc agagctgcca gtgcattggt gtagttatgc tgcccggaaa gtttcatttc
    14101 tttcacgttc agcactttct cgcccttcac ccgcagccag gtttcaccct gctgatgatt
    14161 caggtgatag tcacccatgt tgacgccaaa gctgacacag cgttcatccg caccgcgaat
    14221 cggcattgtt aaggcatcat cagcattaac cacgcaaatt tttgcgtttt cgtaaatgcg
    14281 cagttttgct gcacgatact gttgtaaacc aaacggatag cgatccatat gatcttcagt
    14341 tacgttcaga atggtcgctg ctaccgcctg taaactggag gtggtttcaa gctggaagct
    14401 cgacagctct agcacgtaca gttcgcactc atcatccagc agcatcaacg caggcaggcc
    14461 aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac ccactagagt
    14521 ggtgaccgtg cttttgccgt tagaaccggt gatcgccaca atcggtgctt gtgcttcacg
    14581 acagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag cgcttaagga
    14641 tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca tcagccattc
    14701 atcattcaga ccgcccgtgt ggcgttctac ggcttcgggt aatttatcca ggccaggcgg
    14761 tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa agtccacgca
    14821 agaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctggt aatcagccat
    14881 gattaacgta ccttcagcgt tgccagacca atcaggacca gcatcagcga aataatccag
    14941 aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg atgaatcggt
    15001 gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag gatgacggaa
    15061 agtgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg acgcagcagt
    15121 acggcgataa tgcctaacgc accacctaac gccagcgaac cgacatcgcc cataaagacc
    15181 tgcgccggat aggtgttaaa ccacaggaag cccagccctg ccccgactat cgcggtacag
    15241 acaatgacca gttccccggc gtgtcgtaga tacggtatgt gtaaatagct ggcaaagttc
    15301 atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac ggtcggcata
    15361 attgccagac cgtcgagacc atcagtcagg tttaccgcgt tgccagtacc cacaatgacg
    15421 aagtaagcca gcagaatgta gaacagcccc agttgcggca tcacatcttt aaagaacgga
    15481 accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagcgcgaa ggcaacaccc
    15541 agcgcgatca ccgacatcca gaaatacttc caacgagcga tcaacccttt ggtgtcttta
    15601 cgcaccactt tgcgatagtc atcaacaaag ccaatgatgc cgtaacctac cagcaccacc
    15661 agtacgcacc agacgtacgg attggatggg taagcccaca gcagtacgga tatcacaatc
    15721 gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa gtgtgattca
    15781 ggaccgtcgt tacgcactac ctgaccaaag gaaagttttt gcaaatgagc aatcatacgc
    15841 gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc gcgaaacgtc
    15901 agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc cagccaaact
    15961 aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca ctacgtgaac
    16021 ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaata cgcgcgataa
    16081 gcgctgtttt atctgcaaaa tgttcgccaa cgccgctggc ggtgctgata gcatggcttt
    16141 gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg cccacctgta
    16201 catggcaggc ttcgctttca gcgcccaatt ccgccatatc gcccaccacc agcacgcggt
    16261 agcccggcat ttcagccagt acctggactg ctgcggtcat tgaaccgaca ttggcgttgt
    16321 aggagtcgtc gagcagcaac tggttttccg ccagttggat ggggaacaga cggcctggaa
    16381 cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc acggacatgg
    16441 agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccctggaagc ggcagcagaa
    16501 catcgacgct acctgttggg gtttggaggg taaattccgt accgtgcgaa gtcacatgga
    16561 tgttggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac actttgcgtg
    16621 agccaattac gctctgccag ttcagccagt cgttgttgtc agcgttcatg atggcgatac
    16681 cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc gcaagcgagc
    16741 caaaaccttc cagatgtgcc gctgccaggt tgttgaccag cgccgcttcc gggcgagtca
    16801 gactcacagt ccaggcaatt tcgccctgat ggttcgcgcc aagttcaata actgcgaaat
    16861 cgtattccgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgttg ttgagattgc
    16921 ctgccgtata aagtgtgttg ccgcactggc ttaaaattgc cgccgtcatc tctttaacag
    16981 aggttttgcc ggaggagccc gtcagagcaa ccacgcgcgc cggaacttgc tggcgaaccc
    17041 atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc ggcagatcga
    17101 tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc agctttcgcc tggtcggcaa
    17161 aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa caggcagccc ggcgtcagtt
    17221 ttcgcgtgtc agtggttaca gcatcaaggg taatatctgc accttgcagt tcaccgttga
    17281 gaatatcggt aagttggcta agggttacgc taatcatgca atcaccccca gcagacgcgc
    17341 caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt aatcctcatg
    17401 gcctttgccc gcgaccagta ccacatcatt ctctttagcc tgcataacgg cgcaagtcac
    17461 cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc ccgccagaat
    17521 atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtta ccaccgccac
    17581 gtcagcaaac tcttcagcaa ttgcgcccat cagtggacgt ttgcctttat cacgatcgcc
    17641 accacagcca aagacgcacc acagcttgcc cgcacagtgc aggcgcgccg cctgtaaggc
    17701 tttttccagt gcatccggcg tatgcgcgta atccaccact accgtcggtt tgcctggcgc
    17761 agtgaacact tccatacgtc cacaaaccgg ctgcaggcgt gcggcagttt tcagcaggtc
    17821 agacaacgga tagcccagcg ccagcagcgt cgccagcgcc agcagcaagt tgctgacgtt
    17881 aaaagcgccc atcagatggc tttcaatttc gccatcgccc caacttgaac taaagcgaat
    17941 cgtcgcaccg ctgtcgtgat agttcacgtc ggtcgctttc aaccagcgtc cgtgacagtt
    18001 cggattgata tgatcttcca ttgataccgc aaccgcgtcc gacagttttg ccagccagcg
    18061 gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgct cagaataaag
    18121 cagccatttc gcggcttcgt agtgttccat atcaccatga taatcaaggt gatcgcggct
    18181 taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct gtaccagtcc
    18241 gtgggaggaa acttccattg cgcaaaaagt cgcgccctga tccaccagcc ccgccagctc
    18301 atgctgaaca tcgactgccg aaccggtggt attttctgtc gggatcactt tccccagcag
    18361 accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc tccactgcgc
    18421 cagcagctgg gtcgtcgtgg ttttgccgtt ggtacccgtt acgcctacga gacgtaaatt
    18481 gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcat tgagctggct
    18541 gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg cctcatcttt
    18601 cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat atcgacgccc
    18661 gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca cacggctgtc
    18721 gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag caagaaggtc
    18781 gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat tcatttttat
    18841 cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg cccatgatgg
    18901 caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc ggatcgttga
    18961 taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg gtataagcaa
    19021 tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg gttttaatgg
    19081 cgatacgata gcccttaatc gccgccttca cgccgccacc acccggtagc gccacgcttt
    19141 ccatcatatg caccacggtg cgaacaatgg attccgggaa gactcgttca ccgggaaccg
    19201 gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg atagttgcgt
    19261 agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag aaggtggccc
    19321 tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct ccgaccaacc
    19381 ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac gctgaggacg
    19441 gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat accccggtca
    19501 gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt cgataaggaa
    19561 tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc atcaccacca
    19621 tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta cgcatcgcct
    19681 ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg actgttagcc atcgccagca
    19741 cttcaccggt gttgacatcc accagcacgg cgctaccaga ttcagccttg ttaaaggcca
    19801 ccgcgttgtt cagttcgcga taaaccagcg cctgcaagcg ttcatcaata ctcagcgcca
    19861 ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga ccatagcggt
    19921 ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa ctcttctcga
    19981 cgccctcaat cccctgacta tcgacgttag taaagccgat gaggtgagca gtcacttcgc
    20041 cggatggata ataacggcga gattcttcac gcagatgaat ccccggcagt ttcagttttt
    20101 tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag cgccctttcg
    20161 ggttggcgtt aatgcgggct gaaagctgat ccagcggaat attgagcgcg ttagccagcg
    20221 ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc gggtcagccc
    20281 aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca gtaatcatgc
    20341 cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct ttcaccagca
    20401 tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc agcgccagga
    20461 gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt tcttcctgac
    20521 gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct tatttttgca
    20581 ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc gtggcgatcc
    20641 tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg cgccattcaa
    20701 tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt aaacgggtat
    20761 ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac aggcagagtg
    20821 gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca tggcgctcgt
    20881 ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg atcatgcgtt
    20941 cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag ccacctcttc
    21001 ttcgcccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt ttttgagctg
    21061 ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt cacgcataaa
    21121 acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc gcccacccgg
    21181 ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt cactgtttac
    21241 ccaaatgcgc accgcctgga aggtccgggt cgcgggatgt ttaaacttat ctttcaccgg
    21301 cgtagcggca gccacgactt ccgccagttc tttggtgcgg gtcatcggct gttcgcggtt
    21361 acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat aggttttcaa
    21421 tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg actgcccacg
    21481 ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc cgcgttcagc
    21541 atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga tcttgccgat
    21601 aagatcgcgc tcggcaacat attcgcccag cgcggagaaa ggtccgtgga tgatggagaa
    21661 gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat cgatcgccag
    21721 caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac cgcgaccaaa
    21781 agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg cttcatccag
    21841 cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc tgcagtcgct
    21901 ctgataagtc tccagtagcc aactgctctg cgtcgatatc ttccttgacc tgttgatgcc
    21961 aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc acttcttttg
    22021 tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg ccatccatct
    22081 gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc gggttcatgc
    22141 tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg taaagcagca
    22201 ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc tcaagcagct
    22261 gctcccgata acgggtaggc actgataagc gccctttgct gtcgagattg actaacgttg
    22321 ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat ttcccactga
    22381 aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat accgaggtaa
    22441 aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac gtgcgaagca
    22501 aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcaccg ttttaaaatg
    22561 actaactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg gcattttagg
    22621 acatatcttc cccggttaac agtccctgtt gcgtctgtgt ggcgcttgat gccagcgggt
    22681 ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatctctgc atgtaacaaa
    22741 ataatgcaaa aacgcgctat cgatatcact taattattgt gaatttcatc tggtgaggta
    22801 attaacgggc aggtttataa gcatttatca ataatagcca ggaatagtct tatttacttt
    22861 aaggatattg atgtccattc ccgtactcaa cgcgccagag agaaattcac ctgacgcgta
    22921 tttttgttcg cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg
    22981 cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc
    23041 acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca ctgtaagaag
    23101 tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtca
    23161 cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt gaacagtgcc
    23221 tgcggcatcg gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca
    23281 tagctgttgg cataaaggaa atgcacttcg cgcggatcat ctttccaggc agtacggaaa
    23341 ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata aagtaccgtc
    23401 tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca
    23461 accacgctgg tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg
    23521 tcgttagccc agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg
    23581 gcatcaacct gacgctgcaa aagatgctcg atacaacgca tttcgttgtc gggctgatct
    23641 tccgagcagg caatcagcag ttgataaccc ctttgccgcg cctggcgttc aagatagtta
    23701 gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg
    23761 cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca
    23821 gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata
    23881 acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca
    23941 attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat
    24001 taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat
    24061 ttcgcaaaaa aagcccaacg tgacatgttg ggctgcaaat tgcgcattga gatcattctc
    24121 aacgcattat tttatcgccg cgcgaaagtc cgactacacc agaacgagca acttccacaa
    24181 ttttcgccac atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta
    24241 attgaacggt ataaagcgag ggtgtgacat caataatttg cccacggaat atttccgtat
    24301 tacgtttaac ttcgtcacgc ccgtagccgc tggcctgaat tttcaccagc atgatttccc
    24361 gctcaacatg cgcgccctgc cctaactcgc tcacgcgtaa tacatcgact agcttgtgta
    24421 attgcttttc gatctgctca agcacttttt catcgcccac ggtctggatg gtcatacgcg
    24481 ataatgtcgg atcgtcagtt ggtgcaacgg tcaggctttc aatgttgtag cctcgctggg
    24541 aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata
    24601 tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc
    24661 gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtgacatcaa caaacaccag
    24721 gcgattatta cgcacctgtt ccagcgcctc gctaagtttg ctttccagtt cttgcggatg
    24781 agcaatctgg atcccgacgt gaccataggc ttccgccaga cgaacgaaat cgggtagaga
    24841 ttgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat
    24901 ccccagatag cggttattga gattcactac cagtaccggc aactcgtatt gcaacgcggt
    24961 agacagttcc tggatgttca tctgaatact gccgtcgccc gtgacgcaaa ccacggtttc
    25021 ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag
    25081 gccgccagag ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat
    25141 ctggtgctgc ccgacatcgg acgtcacgta agcgtctccc ttcgtcaacc gccaaagagt
    25201 ctcgatcacc gcctgcggtt taatcttttc actgtgagag tcatatttca ggcactgacg
    25261 agcgcgccac tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatggac
    25321 ggattcttgc gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat
    25381 cgggatatca gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac
    25441 cgtggcattt gggcagtact tcgccagatt gttcgtcgtt cggtcatcaa aacgtacccc
    25501 gacggcaaaa atcacatccg cgttatgcat cgtcatatta gcttcgtagg tgccgtgcat
    25561 tcccagcatg cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccattaatga
    25621 ggaaacaacg ggcagattca acgcctccac cgtttctttt agctgctgat gacagcccgc
    25681 cgtgattgcc ccaccgccta catagacaac cggttttttt gccgccacca gcgtttgcag
    25741 agcgcgctta atttgccctt tatgcccgga ggtcgtggga ttgtaagatc gcatactgac
    25801 cgactccggc cagatgtagg gtaatttgtt cgccggatta agaatatctt tgggtaaatc
    25861 aacgaccacg ggcccaggac gaccacttgc cgccagccag aaagcctttt tcagcacctg
    25921 cggaatgtct tccgtttgct taaccagaaa actgtgttta accaccgggc gagaaatccc
    25981 caccatgtcg cactcctgaa aggcatcgta gcctatcaac gaggtcgcta cctgcccgga
    26041 aaggacaact aagggaatgg aatccatata agcggtggca atgccagtaa tcgcattggt
    26101 cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc gcgccagacc
    26161 atcggccata tgcaccgccg cctgctcatg acgaactaaa acatgatcga taccacccac
    26221 ggtatgcaat gcatcgtaaa tatcaaggac cgcgcctccg ggataaccga atacttgttt
    26281 aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc
    26341 ctgcctcact gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa
    26401 gcaattggca gcctgagaca taaacacggg gtaataagaa taaacaggag gaaaggtgaa
    26461 aagaaacgat aaaatcctcc attacagagg attaaattga taataaatcg gctgaatccc
    26521 acaacttaca gcaatttctc tattttttta attaatccta aaatagcgaa atcacacatt
    26581 cagaataaaa ttctacattc aataaaaaat gcagaacaaa ccagacatta atgtctgacc
    26641 tattctgcat tcagttagcg tttgcaaatt gagactaatt gctcttccat ccactgatgg
    26701 cctttatcgc gcccggcagc ttcatgccag gagagataac aggttctgct gttttgtttt
    26761 aacggcagcg gtaatacctg taattctaag gattcagcga actcttccgc cagccaacgc
    26821 ggcgcaatag cgaccagatg cgtttgcgac accacgctaa gtacgctcat cattgccatg
    26881 ccctgatacg cgatactggc ttgcttatct accgtgtcat accacggttg actaaatgac
    26941 gcgaaacgat cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac
    27001 gggcctttaa ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat
    27061 ggcacactgg taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc
    27121 tgataacgca gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata
    27181 tttggcgcaa tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg
    27241 ctgcagacac aaagatgaaa tacacgttca ctactggcag gctcaaagcc tgaacctggt
    27301 aattcatttt gtactaattg caatgcctga cgaactgaac caaaaagttg aaatgcgcga
    27361 gcagtcggtt gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc
    27421 aggcgtgcaa cagcgttact gaccgcaggt tgcgacattc ccagaacatg agcggcacgg
    27481 gtaatgtttt gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg
    27541 cgtagctgtg gcttgcttaa ctccgccgtc tctggatgat ctgtttgtac ctctggcata
    27601 cttaactcca ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct
    27661 cgagaatatg atttatcatg catataaaat aagaaaaagg aaaatgagta aaattaggaa
    27721 aacataaaga tacgggttct tataaaaaca gaatccattg ccgtggaaag cgtcatttac
    27781 cacagcataa taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga
    27841 atactcaatc atccacaaaa tggattaaat gtgctttttt aataattaat ttatatgttt
    27901 aatgcattaa tccttaacat taattgatca ataatattca ccaaatcaat atcaaaaaat
    27961 tcgcaaaaca tataattcaa tacaaatcat caggataggt tttgtaacgc gtgcatttta
    28021 tccccctttc cctcgtcgat tagatgcaaa aatttattct gaaatatgtc aaccgatgaa
    28081 aagcggcggt ggttaagcag aaattaatat cgcttgtttt aaccaccgca gcacaattag
    28141 ctaatttgac ggatgcagaa ctcatgctgg cgggacgttt ttattacgtc agggttgaca
    28201 tccgtttttg tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg
    28261 actcacatcg ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga
    28321 cgagtgagcg gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg
    28381 cgcgggtttt tttatgcccg aagcgaggcg ctcaaaaaga gacaaggacc caaaccatga
    28441 gccagcaagt cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa
    28501 gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg
    28561 tgatggaagt cgggttcccc gtctcttcgc cgggtgattt tgaatcagtg caaaccatcg
    28621 ctcgccaggt taaaaacagc cgcgtatgcg cgttagctcg ctgcgtggaa aaggatatcg
    28681 acgtggcggc cgaatctctg aaagtcgccg aagccttccg gattcatacc tttattgcca
    28741 cttcgccaat gcacattgcc accaagctgc gtagcacgct ggacgaggtg atcgaacgcg
    28801 ctatctatat ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag
    28861 atgccggacg caccccaatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg
    28921 gtgccaccac catcaacatc ccggacaccg tgggctacac catgccattt gagttcgccg
    28981 gaatcatcag cgggctgtat gaacgcgtac ccaacatcga caaagccatt atttccgtac
    29041 atacccacga cgatttgggc ctggcggtcg gaaactcact ggcggcggta catgccggtg
    29101 cacgccaggt ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttccctgg
    29161 aagaagtgat catggcgatc aaagttcgta aggatattct caacgttcat accgccatta
    29221 atcaccagga aatatggcgc accagccaat tagttagcca gatttgtaat atgccgatcc
    29281 cggcaaacaa agccattgtt ggcagcggcg cattcgcaca ctcctccggt attcatcagg
    29341 atggtgtgct gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtttga
    29401 accaaatcca gctgaatctg acctctcgtt cgggacgtgc ggcagtaaaa caccgcatgg
    29461 atgagatggg gtataaagaa agtgaatata atttagacaa tttgtacgat gccttcctga
    29521 agctggcgga caaaaaaggt caggtgtttg attacgatct ggaggcactg gccttcatcg
    29581 gtaagcagca agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct
    29641 ctaacgatat tgccactgcc gccgtcaaac tggcctgcgg cgaagaagtc aaagcagaag
    29701 ccgccaacgg taacggtccg gtcgatgccg tctatcaggc gataaaccgc atcactgact
    29761 ataacgtcga actggtgaag tacagcctga ccgccaaagg ccacggtaaa gatgcgctgg
    29821 gtcaggtgga tattgtcgcc aactacaatg gtcgccgctt ccacggcgtc ggcctggcca
    29881 ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt acttaacaat atctggcgtg
    29941 ccacagaagt cgaaaaagag ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa
    30001 ccgtgtgatg tcgaagaatt accatattgc cgttttgccc ggagatggga tcgggccgga
    30061 agtgatgacc caggcgctga aagtgctgga tgccgtgcgc aaccgctttg cgatgcgcat
    30121 caccaccagc cattacgatg taggcggcgc agccattgat aaccacgggc agccgctgcc
    30181 gcctgcgacg gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggtgg
    30241 tccgaagtgg gaacatttgc cgccagacca gcaaccagaa cgcggcgcac tgttgccttt
    30301 gcgtaaacac ttcaaattat tcagcaatct gcgtccggca aaactgtatc aggggctgga
    30361 agcattctgt ccgctgcgtg ctgacattgc cgctaacggc ttcgacatcc tgtgtgtgcg
    30421 cgaactgacc ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gtggacaata
    30481 tgaaaaagcg tttgataccg aggtgtatca ccgttttgag attgaacgta tcgcccgcat
    30541 cgcgtttgaa tctgctcgta aacgtcgcca taaagtgacg tctatcgaca aagccaacgt
    30601 gctgcaatcc tctattttat ggcgggagat cgttaacgag atcgccacgg aatacccgga
    30661 tgtcgaactg gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc
    30721 acagtttgac gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc
    30781 aatgatcact ggctcaatgg ggatgttgcc ttccgccagc ctgaacgaac aaggttttgg
    30841 tctgtatgaa ccggcaggcg gctccgcacc agatatcgca ggcaaaaaca tcgccaaccc
    30901 gattgcacaa atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc
    30961 ggcttccgcc attgaacgcg ccattaaccg cgcattagaa gaaggcattc gcaccggaga
    31021 tttagcccgt ggcgctgccg ccgttagtac caatgaaatg ggcgatatca ttgcccgcta
    31081 cgtggcagaa ggggtgtaat catggctaag acgttatacg aaaaattgtt cgacgctcac
    31141 gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat
    31201 gaagtgacct caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag
    31261 ccggggaaaa ccttcgccac catggatcac aacgtctcta ctcagaccaa agacattaat
    31321 gcctgcggtg aaatggcgcg catccagatg caggagctga tcaaaaactg caaagagttt
    31381 ggcgtcgagc tgtatgacct gaatcacccg tatcagggga tcgtccatgt aatggggccg
    31441 gaacagggcg tcacattgcc ggggatgact attgtctgcg gcgactcgca taccgccacc
    31501 cacggcgcgt ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg
    31561 gcaacgcaaa ccctgaaaca gggtcgtgcg aagaccatga aaattgaagt ccagggcaaa
    31621 gccgcgccgg gcattaccgc gaaagatatc gtgctggcga ttatcggtaa aactggcagc
    31681 gcaggcggca ccgggcatgt ggtggagttt tgcggcgaag caatccgtga tttaagcatg
    31741 gaaggtcgta tgaccctgtg caatatggca atcgaaatgg gcgcgaaagc cggtctggtt
    31801 gcaccggacg aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa
    31861 gatttcgacg acgccgttgc ctactggaaa actctgcaaa ccgacgaagg cgcaactttc
    31921 gataccgttg tgactttgcg ggcagaagag atttcaccgc aggtcacctg gggcaccaat
    31981 ccaggtcagg tgatttccgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg
    32041 gttgaacgcg cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg
    32101 ctgacagaag tggctatcga caaagtgttt atcggttcct gcactaactc gcgtattgaa
    32161 gatttacgcg cggcggctga aatcgccaaa ggacggaaag tcgcgccagg cgtgcaggca
    32221 ctggtggttc ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggacaaa
    32281 atctttattg aagccggttt tgaatggcgc ttgccaggtt gctcaatgtg tctggcgatg
    32341 aacaacgatc gcctgaatcc gggtgagcgt tgtgcctcca ccagcaaccg taactttgaa
    32401 ggccgccagg ggcgtggagg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct
    32461 gcggtgaccg gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga
    32521 gaaatttatc aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga
    32581 tgcaatcatc ccgaaacagt ttttgcagaa agtgacccgt actggttttg gcgcgcatct
    32641 gtttaacgac tggcgttttc tggatgaaaa aggccaacag ccaaacccgg acttcgtgct
    32701 gaacttcccg cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgcgg
    32761 ttcctcgcgt gagcacgcgc cgtgggcatt gaccgattac ggttttaaag tggtgattgc
    32821 cccgagtttt gctgacatct tctacggcaa cagctttaac aaccagctgc tgccggtgaa
    32881 attaagcgat gccgaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggattca
    32941 ttttgatgta gacctggaag cgcaagaggt gaaagcggga gagaaaacgt atcgctttac
    33001 catcgatgcc ttccgccgcc actgcatgat gaacggtctg gacagcatcg gcctgacatt
    33061 gcagcacgac gacgccattg cgtcttatga agagaagcaa ccggcattta tgcgttagtt
    33121 tgtaaaccgt ccggcactca atcgccaaaa agaaaaaagc cagcagataa tcactgctgg
    33181 cgtggcgatt aacaccaata ctcagtcaca catgatgtcc gcaaatcagt agtcagaatg
    33241 actcccctcc cactgcatca gttcttcaag catagttaat cgttgtggga cgcttaacca
    33301 acgcaaccag cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact gacgcatggg
    33361 gcacctcctt gcttcatcgt taggaattat tgacttaata tagggaaaat aaaattgctg
    33421 tcttttgcgc aggagttccc cttttatgcc atctgctcgt ctgcaacaac agttcatccg
    33481 cctgtggcaa tgctgcgagg gtaaatcgca ggacacaacg ctcaacgaac tggcagcgtt
    33541 attgagctgc tcgcgtcgtc atatgcgtac cctgctcaac accatgcagg atcgcggctg
    33601 gctgacgtgg gaagcggaag tcgggcgcgg taaacgctcg cgtctgacat tcctctatac
    33661 cgggctggcg cttcagcaac agcgggcgga agacctgctg gagcaggatc gtatcgacca
    33721 actagtgcag ttggttggcg acaaagcgac tgtgcggcaa atgctggttt ctcatctggg
    33781 ccgcagcttc cgccaggggc ggcacatcct gcgcgtgctc tactatcgtc cgttgcgtaa
    33841 tctgctacct ggcagcgcat tgcgccgttc cgaaacccat attgcccggc aaatcttcag
    33901 ttcgctaacg cgcataaatg aggaaaatgg ggaactggaa gcagacatcg cccaccactg
    33961 gcagcaaatt tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc attttcatca
    34021 tggtcgtgaa ctggaaatgg acgacgtgat cgcctcttta aaacgaatca atacgctgcc
    34081 gctctattcg catattgctg acattgtgtc gccgacgccc tggacgctgg atatccatct
    34141 cacgcaaccg gaccgctggt taccgttact gctggggcaa gttccggcga tgatcctgcc
    34201 gcgcgaatgg gaaaccctca gcaactttgc cagccatccc atcggaacgg gtccttatgc
    34261 ggtgatccgc aacagcagca atcaactgaa aattcaggca ttcgacgact tctttggtta
    34321 tcgggcatta atcgacgaag ttaacgtctg ggtcttgccg gaaattgctg atgaccccgc
    34381 gggaggcctg atgctgaaag gaccgcaaga cgaggaaaaa gagattgaaa gccgccttga
    34441 agaaggttgt tactatttac tgttcgacag ccgtacccat cgcggggcga atcagcaagt
    34501 cagagactgg ataagctatg tgctttcgcc gaccaatctg gtttatttcg ccgaggaaca
    34561 gtaccagcag ctgtggtttc cggcctatgg gctgcttccc cgttggcacc atacccgcac
    34621 cataaagagc gaaaaaccgg ctggtctgga aagcctcacc ctgacctttt atcaggatca
    34681 cagtgagcat agggtgattg ccgggatcat gcagcagatt ctggcaagtc accaggtcac
    34741 gctggaaatc aaagagatca gctacgatca gtggcatgaa ggagagatcg agagcgatat
    34801 ctggcttaac agcgccaact ttacgctgcc gctggatttt tcgctattcg cgcacctgtg
    34861 cgaggtgccg ctgctccaac actgtattcc gatcgactgg caagccgacg ccgcccgctg
    34921 gcgcaatggc gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcca gcaaagcaat
    34981 ggtgccgctt atccaccact ggctgattat tcagggacaa cgcagtatgc gcggcctgcg
    35041 catgaacacc ctcggctggt tcgattttaa atcagcgtgg tttgcgccgc cggatccaga
    35101 gtagcttgcg ccttttggcg gtcatgagcg cattttttat atttagacgg aatgaataaa
    35161 atacattact tttatatttt ctctgtttta tgcatcgtct gattagagtc gcaaataaga
    35221 ttatctcgaa tcgtgtgcga ttcattgtct gaaaaatgac gactccgcac tccctgaaaa
    35281 caaggcagag tctggattcg actccagact ccaactcaaa tttcttttgc cagaataaaa
    35341 atacacgctc ataatttcag taacacgctg ttgtttgagg ctaatcagac gagattttat
    35401 cttgcgtggg tgtgggatgt tgatgttaag tgacaggcat atgaaaatta gcataccggg
    35461 tagaattcag tatttctgcg caatattgtt ttatataaaa aataagtcag tgtgtttttt
    35521 accttatttt atatatagtg gtcagcacca acttaaaaaa gaaagggcgt tcctgcccct
    35581 tccctattta ttattctggc tcaatcaata attgcgccac gactgcgcaa tttagcgcta
    35641 tcctggccgg aaaggtcagc cttgctcagg ttaatcccgc gcacgttagt atcttccata
    35701 taagcattgt tcaggatggt attgtccatt tttgcattgc gcaggagtgt atcgtccagg
    35761 tcagtatcgg acatgtcagt atcggacagg ttagtatcgg acaggtcaga gctaataact
    35821 ttggcattgt tcattttagc accgtgcagg tcagcaccga gtaggttaga actgcacaga
    35881 ctgatcttgt ccatttcagc attgctcagg tcagccttgc gaaggcaagc accattaagg
    35941 gaaatcccgg aaaggtaagc attactcaga tcaatgcccc cggtactggg gcttattccc
    36001 aattgcaact tctgagtata tatctctata tttccaatat aaaaactgac acataacata
    36061 ttatctggcg agattgagcc cctcgcctgc atcatttccg gatcttttgc gtgttctttg
    36121 agttcattaa atttttttag ccggtgggta aacctatcac gcgccttctc gtcgacagcc
    36181 ccggtaaagt attttgagta gccatcacaa ttctggaaat aaaatgcatc cttcagaatt
    36241 tgccttgccg cagtctggga atgtttatct gaccggaaga gattgcgtaa cgtctcccat
    36301 agctcttcaa agatattcat attctcggtc tttttgacca ggttaaccat cctgataatc
    36361 tgagagtgag taaggcctgg atctttagtg gtattacttg cagggatact aatctgttct
    36421 gggtttctta ctttcatgct gatagctcca ctatgagtta aaaactcaga ataagcgtcc
    36481 tgaataagaa tactgtcaaa aatcgctcat tcacgcaata agccgttgca ttaccgattt
    36541 tgactgtgct aagggattct ttcggttcaa agcttcccag ctatgcaaat acgtccttga
    36601 tagctgaacg aatattggct tttttaatac gccttctcta tggattgctg gttgataaca
    36661 aaatcactac actaacgccg ttctcaacgg ggtgccacgc agacgcgtgc gctgagaaaa
    36721 tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc ttctcaagtc
    36781 ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat atcttcccct gctgttgctg
    36841 tgcacagcgc ccgttttcgc gaaacctgtt ctgactgttt atacctacga ttccttcgcc
    36901 gctgactggg ggccaggccc agtggtaaaa aaagcctttg aagccgactg taattgcgaa
    36961 ctgaaactgg tagcgctgga agatggcgtt tcgcttctca accgtctgcg gatggaaggc
    37021 aaaaacagta aagctgatgt ggtgctgggg ctggataaca atctgttaga cgccgccagc
    37081 aaaaccggac tgtttgccaa aagcggtgtg gcagcagatg cggtaaatgt acccggcggc
    37141 tggaataatg acactttcgt accgtttgat tacggctatt tcgccttcgt ctatgacaag
    37201 aacaaactga aaaatccgcc gcaaagcctg aaagaattgg ttgagagcga tcaaaactgg
    37261 cgggtgattt atcaggatcc gcgcaccagt acgccggggc tgggcctgct gttgtggatg
    37321 caaaaagtct atggcgatga cgccccacaa gcctggcaga aactggcgaa gaaaacggtc
    37381 acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggcga aagcgatctg
    37441 gtactgagtt acaccacctc tccggcttat cacattctcg aagagaagaa agataactac
    37501 gccaccgcga acttcagcga aggtcactat ttgcaagtgg aagtcgccgc ccgcaccgct
    37561 gccagcaagc agccagagct ggcgcaaaaa ttcctccagt ttatggtttc tccggctttc
    37621 cagaatgcga tcccaaccgg caactggatg tatccggtgg caaatgtcac gctgcctgcc
    37681 gggtttgaac aattgaccaa accggcaacc acgctggagt tcacgccagc cgaagtggcg
    37741 gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta attcccggct
    37801 ggttaattcc aggtgtaagc gccgctacgc ttgtggtggc ggttgcgctg gcggcgtttc
    37861 tcgccctgtg gtggaacgcg ccgcagggtg actgggtggc aatctggcag gacagctacc
    37921 tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta ctctctgtcg
    37981 tacccgcgat attcctcgcc cgcgcgctct atcgcaggcg ctttccgggt cggctggcgc
    38041 tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct ggttgctgtt ttcggcattc
    38101 ttagcgtcta tggtcgccag ggctggctgg catcgctctg ccaatcgctc ggtctggagt
    38161 ggaccttttc gccctacggc ctgcaaggca ttttgctggc ccatgtgttt tttaatctgc
    38221 cgatggcgag ccgtttatta cttcaggcac tggaaaacat tcccggcgaa caacgtcaac
    38281 ttgccgccca gcttgggatg cgtggctggc acttctttcg tttcgtcgaa tggccgtggt
    38341 tacggcgaca aatcccacca gttgctgcgc ttatctttat gctctgtttc gccagcttcg
    38401 ccaccgtgtt gtcgctgggg ggcggtccgc aggcgaccac tatcgaactg gctatttatc
    38461 aggcgctgag ttacgactac gatcccaccc gcgcggcgat gctggcgctg atccagatgg
    38521 tgtgttgcct tgggctggtg ctgttgagtc agcgattgag taaggccatt gcgcccggca
    38581 ccacgctgct gcaaggctgg cgcgacccgg acgatcgtct gcatagccgc atttgcgaca
    38641 cggtgttaat tgtgctggcg ctgttgctgt tgctgccacc gttactggcg gtgctcgtcg
    38701 atgggataaa tcgccagttg ccggaagtgc tggcgcagcc ggtgctgtgg caggcgctgt
    38761 ggacctcgtt gcgtattgcg ctggtggcag gcgtattatg cgtagtgctg accatgatgc
    38821 tgctatggag cagtcgcgaa ctgcgggcgc ggcaaaaaat gctggcgggt caggcgctgg
    38881 agatgagcgg tatgttgatc ctcgccatgc cggggattgt gctggcaacg ggcttctttt
    38941 tgctgctcaa caacaccatt ggcctgccac aatctgctga cggcattgtg attttcacca
    39001 atgcgttaat ggcgatccct tatgcgctga aggtgctgga aaacccgatg cgcgatatca
    39061 ccgcgcgtta cagtatgttg tgtcagtcgc tgggcattga aggctggtca cgcttaaaag
    39121 tggtggagct gcgcgccctg aaacgcccac ttgcgcaggc actggccttt gcttgtgtgc
    39181 tatcgattgg tgattttggc gtggtggcgt tgttcggtaa cgatgatttc cgcaccctgc
    39241 cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg gtcaccgcgt
    39301 taattctgct gctactctgt tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa
    39361 atgttaaaac tgactgatat cacctggctt taccaccatt tgccgatgcg ttttagctta
    39421 acggtggaac ggggcgagca ggtggcgatc ctcgggccaa gcggcgcggg taaaagtacc
    39481 ctgctgaatt tgatcgccgg ttttctgacg ccagccagcg gttcgctgac tatcgacggc
    39541 gttgatcaca cctctacgcc gccgtcacgc cgtccggtgt cgatgctgtt tcaggagaac
    39601 aacctgttca gtcacctgac ggtcgcgcag aacatcgggc tggggcttaa tccaggattg
    39661 aagctgaacg cggcacaaca ggagaaaatg cacgctatcg cccgccagat ggggattgat
    39721 aatttaatgg cgcgattacc gggcgagctt tctggcggtc aacgacagcg cgtggcgtta
    39781 gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt ctctgcgctc
    39841 gatccggcgt tacgtcaaga gatgttgacg ctggtgagca cgagctgcca gcagcaaaaa
    39901 atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg
    39961 gtggtggtcg ccgacgggcg catcgcctgg cagggtatga ccgatgagtt gttgagcggt
    40021 aaggcgagtg cttcagcact attggggatt acgggttagt atgcactgtc ggatgcggcg
    40081 tgaacgcctt atccgaccta cggacagtgc gttttgtagg cctgataaga cgcggcaagc
    40141 gtcgcatcag gcaacccgca caaggctatc ccctaacccc caaccacttt acgcaaaata
    40201 tcgatataca tcggcatcaa cgggtggcga attaacacca ccagcgccac tacaccgacg
    40261 gcagaaatca acggcgtcag ccacaacaaa cgaccgcgcg acaaataatg acttaaacga
    40321 tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaat
    40381 accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc tggaatatcg
    40441 atcgccgcac ccgccagaat ccccggcagg aagtaaaacg gtggccacag caggcagccg
    40501 ataatattcg gcgtaataaa ttttgccacc gggagatcca gcattcccgc caccattggc
    40561 accagcggac gcgtcgggcc aacaaaacga ccgaccagaa tggtgaacat gctgtgttga
    40621 tgcaacgcat gttcagtttt atcaagtagt gctttgtttt tcttcagaaa tgaccagcga
    40681 tgcaatggct ttttaaaacg ccagcccagc cagaaagaga tccagtcgcc cagcaggcaa
    40741 ccgacgatcc ctgccagcca ggcgtgccag aaacttaact cgccactgcc aatcagcgcc
    40801 cccagtcccg ccattagcac cgtaccgggt aaaatcaaac cgaccagcgc cagcgactcc
    40861 agaaaggcca ccaacaccac cgctatcaat gaatacacgg tggattgggt aataaagtgt
    40921 tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
    40981 atgctactcc gtcaagtcgt caattgtctg attcgttacc aattatgaca acttgacggc
    41041 tacatcattc actttttctt cacaaccggc acggaactcg ctcggactgg ccccggtgca
    41101 ttttttaaat actcgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
    41161 agcaataggc atccgggtgg tgctcaaaag cagcttcgcc tgactaatgc gttggtcctc
    41221 gcgccagctt aagacgctaa ttcctaactg ctggcggaac agatgcgaca gacgcgacgg
    41281 cgacaggcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
    41341 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
    41401 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
    41461 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
    41521 ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg gccagttaag
    41581 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
    41641 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
    41701 tcggcagaca aattctcgtc cctgattttt caccaccccc tggccgcgta tggtgagatt
    41761 gagaatataa cccttcattc ccagtggtcg gtcgataaaa aaatcgagat aaccgttggc
    41821 ctcaatcggc gttaaacccg ccaccagatg ggcgttaaac gagtatcccg gcagcagggg
    41881 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
    41941 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaatcca
    42001 accggtaacc ccgcttatta aaagcattct gtaacaaagt gggaccaaag ccatgacaaa
    42061 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
    42121 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
    42181 ctttttttcg caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga
    42241 tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact
    42301 gcgccaccgg tgaagagatc gccaccagcg tagagtggta tccccgttgg cagaaagggc
    42361 aattttgtga tgccccaaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa
    42421 tggaagcggc gctgaaaacc gtgcttgcag agcttagcgc cgagcagcgc gcagctgtgg
    42481 tcgggattgg cgttgacaca accggctcga cgcccgcacc gattgacgcg gacggtaacg
    42541 tcctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag
    42601 accacaccgc ggttgaagaa gcggaagaga ttacccgctt atgtcacacg ccaggcaacg
    42661 ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatac
    42721 tgcatgtaac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt
    42781 gcgactgggt gccagctctg ctttccggta ccacgcgccc gcaggatatt cgtcgcggac
    42841 gttgcagcgc cgggcataag tcgctatggc acgaaagctg gggcggcctg ccgccagcca
    42901 gtttctttga cgaactggac ccgatcctca atcgccattt gccttccccg ctgttcactg
    42961 atacctggac tgccgatatt ccggtgggca ctttatgccc ggaatgggcg cagcgtctcg
    43021 gcctgcctga aagcgtggtg atttccggcg gcgcgtttga ctgccatatg ggcgcagttg
    43081 gcgcaggcgc acagcctaac gctctggtaa aagttatcgg tacttccacc tgcgacattc
    43141 tgattgccga caaacagagc gttggcgagc gggcagtgaa aggtatttgc ggtcaggttg
    43201 atggcagcgt ggtgcctgga tttatcggtc tggaagcagg ccaatcggcg tttggcgata
    43261 tctacgcctg gttcggtcgc gtactcggct ggccgctgga acagcttgcc gcccagcatc
    43321 cggaactgaa agagcaaatc aacgccagcc agaaacaact gcttccggcg ctgaccgaag
    43381 catgggccaa aaatccgtct ctggatcacc tgccggtggt gctcgactgg tttaacggcc
    43441 gccgcacacc gaacgctaac caacgcctga aaggggtgat taccgatctt aacctcgcta
    43501 ccgacgctcc gctgctgttc ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa
    43561 tcatggagtg ctttaccgat caggggatcg ccgttaataa cgtgatggcg ctgggcggca
    43621 tcgcgcggaa aaaccaggtc attatgcagg cctgctgcga cgtgctgaat cgcccgctgc
    43681 aaattgttgc ctctgaccag tgctgtgcgc tcggtgcggc gattttcgct gccgtcgccg
    43741 cgaaagtgca cgcagacatc ccatcagccc agcaaaaaat ggccagtgcg gtcgagaaaa
    43801 ccctgcaacc gcgcagcgaa caggcacaac gctttgaaca gctttatcgc cgctatcagc
    43861 aatgggcgat gagcgccgaa caacattatc ttccaacttc cgccccggca caggctgccc
    43921 aggccgttcc gactctataa ggacacgaca atgacgattt ttgataatta tgaagtgtgg
    43981 tttgtaattg gcagccagca tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat
    44041 gccgagcacg ttgttaatgc gctgaatacg gaagcgaagc tgccctgcaa actggtgttg
    44101 aaaccgctgg gcaccacgcc ggatgaaatc accgctattt gccgtgacgc gaattacgac
    44161 gatcgttgcg ccggcctggt agtgtggctg cacacctttt ccccggccaa aatgtggatc
    44221 aacggcctga ccatgctcaa caaaccgttg ctgcaattcc acacccagtt taacgcggcg
    44281 ctgccgtggg atagcatcga tatggacttt atgaacctga accagactgc acatggcggt
    44341 cgcgagttcg gcttcattgg cgcgcgtatg cgtcagcaac atgccgtcgt taccggtcac
    44401 tggcaggata aacaagcaca tgagcgtatc ggctcctgga tgcgtcaggc ggtatctaaa
    44461 caggataccc gtcatctgaa agtctgccgt tttggcgata acatgcgtga agtggcggtc
    44521 accgatggcg ataaagttgc cgcacagatc aagttcggtt tctccatcaa tacctgggcg
    44581 gttggcgatc tggtgcaggt agtgaactcc atcagcgatg gcgatgttaa cgcgctggtc
    44641 gatgagtacg aaagctgcta caccatgacg cctgccacac aaatccacgg cgaaaaacgc
    44701 cagaacgtgc tggaagcggc gcgtattgag ctggggatga agcgtttcct ggaacaaggt
    44761 ggcttccacg cgttcaccac cacctttgaa gatttgcacg gtctgaaaca gcttcctggt
    44821 ctggccgtac agcgtctgat gcagcagggt tacggctttg cgggcgaagg cgactggaaa
    44881 actgccgccc tgcttcgcat catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc
    44941 tttatggagg actacaccta tcacttcgag aaaggcaatg acctggtgct cggctcccac
    45001 atgctggaag tctgcccgtc gatcgccgca gaagagaaac cgatcctcga cgttcagcat
    45061 ctcggtattg gtggtaagga cgatcctgcc cgcctgatct tcaataccca aaccggccca
    45121 gccattgtcg ccagcttgat tgatctcggc gatcgttacc gtctgctggt taactgcatc
    45181 gacacagtga aaacaccgca ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa
    45241 gcgcaaccgg atctgccaac tgcttccgaa gcgtggatcc ttgctggtgg cgcgcaccat
    45301 accgttttca gccatgcgct gaacctcaac gatatgcgcc agttcgccga gatgcacgac
    45361 attgaaatca cggtgattga taacgacacc cgcctgccag cgtttaaaga cgcgctgcgc
    45421 tggaacgaag tgtattacgg atttcgtcgc taagtagctg catcaggtat gtaacgcctg
    45481 atgcgacgct ggcgcgtctt atcaggccta cacgctgcga ttttgtaggc cggataagca
    45541 aagcgcatcc ggcattcaac gcctgatgcg acgctggcgc gtcttatcag gcctacacgc
    45601 tgcgattttg taggccggat aagcaaagcg catccggcat tcaacgcctg atgcgatgct
    45661 ggcgcgtctt atcaggccta cgcgctgcga ttttgtaggc cgggtaagca aagcgcatcc
    45721 ggcacgaagg agtcaacatg ttagaagatc tcaaacgcca ggtattagaa gccaatttgg
    45781 cgctgccaaa acataacctg gtcacgctca catggggcaa cgtcagcgcc gttgatcgcg
    45841 agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta caacgtcatg accgctgacg
    45901 atatggtcgt ggtcagcatc gaaaccggtg aagtggttga aggaacgaaa aagccctcct
    45961 ccgacacgcc aactcaccgg ctgctctatc aggcattccc gtccattggc ggcattgtgc
    46021 atacgcactc gcgccacgcc accatctggg cgcaggcggg ccagtcaatt ccggcaaccg
    46081 gtaccaccca cgccgactat ttctacggca ccattccctg cacccgcaaa atgaccgacg
    46141 cagaaatcaa cggtgaatat gagtgggaaa ccggtaacgt catcgtagaa accttcgaaa
    46201 aacagggtat cgacgcagca caaatgcccg gcgttctggt ccattctcac ggcccgtttg
    46261 catggggcaa aaatgccgaa gatgcggtgc acaacgccat cgtgctggaa gaagtcgctt
    46321 atatggggat tttctgccgc cagcttgctc cgcagttacc ggatatgcag caaacgttgc
    46381 tggataagca ctatctgcgt aagcatggcg caaaagccta ttacgggcag taatgactgt
    46441 ataaaaccac agccaatcaa acgaaaccag gctatactca agcctggttt tttattggat
    46501 tttcagcgtg gcgcaggcag gttttatctt aacccgacac tggcgggata ccccgcaagg
    46561 gacagaggtc tccttctggc tggcaacgga caacgggccg ttgcaggtta cgcttgcacc
    46621 gcaagagtcc gtggcattta ttcccgccga tcatgttccc cgcgctcagc atattttgca
    46681 gggtgaacaa ggctttcgcc tgacgccgct ggcgttaaaa gattttcacc gccagccggt
    46741 gtatggcctt tactgtcgcg cccatcgcca gttgataaat tacgaaaagc gcctgcgtga
    46801 aggtggcgtt accgtctacg aggccgatgt gcgcccgcca gaacgctatc tgatggagcg
    46861 gtttatcacc tctccggtgt gggtcgaggg tgatatgcgc aatggcgcta tcgttaatgc
    46921 ccgtctgaaa ccgcatcccg actaccgtcc gccgctcaag tgggtttcta ttgatattga
    46981 aaccacccgc cacggtgagc tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt
    47041 ttatatgctg gggccagaga atggcgacac ctccgcgctc gattttaaac tggaatatgt
    47101 cgccagtcgc ccgcagctac tggaaaaact caacgcctgg tttgcgactc acgatcccga
    47161 tgtgatcatc ggctggaacg tggtgcaatt cgatctacga atgctgcaaa aacatgccga
    47221 gcgttctcgt attccgctgc ggctggggcg cgataacagt gagctggagt ggcgcgagca
    47281 cggctttaaa aacggcgtct tttttgccca ggccaaaggg cgactgatta tcgacggtat
    47341 cgaggcgctg aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ccgtcgctca
    47401 ggagttatta ggcgaaggaa aatctatcga taacccgtgg gatcggatgg acgaaattga
    47461 ccgccgtttc gccgaagata aacctgcgct ggcaacttat aacctgaaag attgcgagct
    47521 ggtgacgcag atcttccaca aaactgaaat catgccattt ttactcgaac gagcgacggt
    47581 gaacggcctg ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc atctctattt
    47641 tccgcgaatg catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc cgccacacgc
    47701 cagccctggc ggctatgtga tggattcgcg gccagggctt tatgattcgg tactggtgct
    47761 ggactacaaa agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct
    47821 ggtggaaggc atggcgcagc ctgatccaga gcacagcacc gaaggttttc tcgatgcctg
    47881 gttctcgcgg gagaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga
    47941 tgaagccaaa cgtcagggta ataagccgct ttcgcaggcg ctgaagatca ttatgaatgc
    48001 cttttatggc gtgcttggca ccaccgcctg ccgcttcttc gatccgcgcc tggcgtcgtc
    48061 gatcaccatg cgtggtcatc agatcatgcg gcaaaccaaa gcgttgattg aagcgcaggg
    48121 ctacgacgtg atctacggcg ataccgactc aacgtttgtc tggttgaaag gcgcacattc
    48181 ggaagaagaa gcggcgaaaa ttggtcgtgc actggtgcag cacgttaacg cctggtgggc
    48241 agaaacgctg caaaaacaac ggctgaccag cgcattagaa ctggagtatg aaacccattt
    48301 ctgccgtttt ctgatgccaa ccattcgcgg agccgatacc ggtagtaaaa agcgttatgc
    48361 cgggctgatt caggagggcg ataaacagcg gatggtgttt aaagggctgg aaaccgtgcg
    48421 caccgactgg acgccgctgg cccagcagtt tcagcaggag ctgtacctgc gtatcttccg
    48481 caacgagcca tatcaggagt acgtccgcga aaccatcgac aaactgatgg cgggtgaact
    48541 ggatgcgcgg ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa
    48601 tgttccgcct catgtacgcg ctgcacgcct tgccgatgaa gaaaaccaaa agcgcggtcg
    48661 ccccttgcaa tatcagaacc gcggcaccat taagtacgta tggaccacca acggcccgga
    48721 gccgctggac taccaacgtt caccactgga ttacgaacac tatctgaccc gccagctaca
    48781 acccgtggcg gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg
    48841 gcaacttggg ctattttgag caaaaaaaga gttcgccaga taccattttg atgcgtgacg
    48901 aatgctttgc catccagtac catagcgccc tttccattcc tggacctgaa taacaccact
    48961 acctcataag cacggtagcg ggtggttatt gcctgcaatt aaagatatag agccgaacac
    49021 atatgccttt tacacttggt caacgctgga tcagcgatac agaaagcgaa ttgggacttg
    49081 gaaccgttgt cgcggtggat gcgcgaactg tcactttact tttcccatct actggtgaaa
    49141 accgtctgta cgcacgcagt gattcccccg tgacccgcgt gatgttcaac cctggtgata
    49201 ccattaccag ccatgacggc tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct
    49261 tgctgactta tatcggtact cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag
    49321 ttttccttga tagcaaactg gtgttcagca aaccgcagga ccgtctgttt gccgggcaga
    49381 ttgaccgaat ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt
    49441 tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatccct catcagctca
    49501 acatcgctca tgatgttggt cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg
    49561 gtttagggaa aaccattgaa gccgggatga tcctgcatca gcaactgctc tctggcgctg
    49621 ctgaacgtgt gctgattatc gtcccggaaa ccttacagca tcagtggctg gtagaaatgc
    49681 tgcgccgttt caacctgcgc tttgctctgt ttgatgatga gcgttatgcc gaagctcagc
    49741 acgatgctta caacccgttc gataccgaac aactggtgat ttgctcgctg gattttgccc
    49801 gtcgtagcaa acagcgtctg gaacatctct gtgaagccga gtgggacctg ctggtggtcg
    49861 atgaagccca tcatctggtg tggagcgaag atgcgccgag ccgcgaatat caggccattg
    49921 aacaactggc agagcacgtg ccgggcgttc tgctgctgac cgcaaccccg gaacagctgg
    49981 ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc cacgatttcg
    50041 cgcagttcgt tgaagagcag aaaaattatc gtccggttgc ggacgccgtt gccatgctgc
    50101 tggcaggtaa caagctgagc aatgacgaac tgaacatgct tggcgaaatg atcggcgagc
    50161 aggatatcga gccgctgtta caagcagcaa acagcgacag cgaagatgcc cagagcgccc
    50221 gtcaggagct ggtttcgatg ctgatggatc gccacggcac cagccgcgtg ctgttccgta
    50281 acacccgtaa cggtgtgaaa ggcttcccga aacgcgagct gcacaccatt aagctgccgc
    50341 tgccgacgca gtatcagacg gctattaaag tctccggcat tatgggcgca cgcaaaagtg
    50401 cggaagatcg cgctcgcgat atgctctacc cggagcgtat ttatcaggaa tttgaaggtg
    50461 ataacgccac ctggtggaac ttcgatccgc gcgttgagtg gctgatgggc tacctgacca
    50521 gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc cgctaccgcg ctgcaactgg
    50581 agcaggtact gcgcgagcgt gaaggtattc gcgccgcggt gttccacgaa ggtatgtcga
    50641 ttattgaacg tgaccgcgct gccgcctggt ttgccgaaga agacaccggc gcgcaggtac
    50701 tgctgtgctc ggaaatcggt tctgaaggac gtaacttcca gttcgccagc cacatggtaa
    50761 tgtttgacct gccattcaac ccggatctgc tggagcagcg tattggtcgt ctggatcgta
    50821 tcggtcaggc gcacgatatt cagatccatg tgccttatct ggagaaaacc gctcagtcgg
    50881 tgctggtgcg ctggtatcac gaaggtctgg atgcattcga gcacacctgc ccgaccggac
    50941 gcactattta cgatagcgta tacaacgatc tgattaacta tctggcttca ccggatcaaa
    51001 ccgaaggctt tgacgatctg atcaaaaact gccgcgagca acatgaagcg ctgaaagcac
    51061 aactggaaca gggtcgtgac cgcctgctgg aaatccactc caacggtggc gaaaaagccc
    51121 aggcgctggc agaaagcatt gaagagcagg atgacgatac caacctgatc gccttcgcca
    51181 tgaacctgtt cgatattatc ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc
    51241 tgacgccgtc cgatcatatg ctggtgccgg acttccctgg cctgtcggaa gatggcatca
    51301 ccatcacctt tgatcgtgaa gtggcgctgg cgcgtgaaga tgcgcagttt attacctggg
    51361 agcatccgct gatccgcaac ggtctggatc tgatcctttc tggcgatacc ggtagcagca
    51421 cgatttcact gttgaaaaac aaagcgttgc cggtaggtac gctgttggtg gaactgattt
    51481 acgtggtcga agcccaggct ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc
    51541 cggtacgtat gctgctggat aaaaacggca acaacctggc ggcgcaggtg gagtttgaaa
    51601 cctttaaccg tcagcttaac gcggttaacc gtcacaccgg cagcaaactg gttaacgccg
    51661 tgcagcagga tgttcacgct atccttcagt tgggtgaagc gcagatcgag aaatctgccc
    51721 gtgcactgat tgatgccgca cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc
    51781 gtctggaagc tctgcgtgca gtgaacccga acattcgtga cgacgaactg accgccattg
    51841 agagcaaccg tcagcaggta atggaaagcc tggatcaggc aggctggcgt ctggatgccc
    51901 tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa atggggatgg aaaactacaa
    51961 tccaccgcag gaaccctggt tggttatcct gtatcaggat gaccatatta tggtggtcaa
    52021 caagccgagc ggtttgttgt ctgtgccggg tcgtctggaa gagcacaaag acagcgtgat
    52081 gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac
    52141 cagtggcgtg attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt
    52201 ccgcgagcgc gagccaaaaa agcagtatgt ggcccgcgtc tgggggcatc catcccctgc
    52261 ggaaggtctg gtggatttgc cgctgatttg cgactggcca aaccgcccga aacagaaagt
    52321 ctgttacgaa acgggtaaac ctgcgcagac ggagtatgaa gtggtggagt atgcggcgga
    52381 taacacggca agagtggtgt taaaaccgat taccgggcgt tcgcatcaat tgcgtgtgca
    52441 tatgctggcg ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag
    52501 agcgatggca ccacgtttat tgctacatgc agagatgctg acgattaccc atccggcgta
    52561 tggcaatagc atgacgttta aagcgccagc ggatttttaa cctttcctgt acgtttatac
    52621 cctcatccta agggctgcct cttgtacata aatccccgag tccggggatt ttttgtttct
    52681 gccaaactgc tttcagttgt tgcgtgtggc tacgctttcc attcggtaat tcccttcctg
    52741 ctccacagcg aatttttgaa ctttttcttc acccgttgat cgtctctatt atttcatttt
    52801 atcgggtctg ttatggtcct ttctgattgt tattcatggg ctaattagca gtctggttgg
    52861 cgatgcccat ctcggtaatc cgcgtcgtgc acgacgcctt gcatcaccag cctcttctct
    52921 agctcatcat gcaggtcttt ctaattgtga aatcatccca cgctactgct aaggttgaag
    52981 gggcgttcca gccccttcaa ccttagctta ttctggatca ttcaataatt gcgttggcaa
    53041 taatttttca gcagtacttt gtttagtggt aatttcttca gtagcaattt ttttggtggt
    53101 gattttatta ataataattg cgtcagtacc gtccaggata gcaccttcca ggttagcacc
    53161 gtccaggtta gcattccacc caggttccaa gtaaagggat ttatcgttac ctttcattat
    53221 gttgggacca aggtaacaat tcctcatgtc aacgccggga aggtaagcct ttctaagatc
    53281 aacgcccaag ctaccgaggc ccttgcacag cttcaacgtc tgagtacata tctcattttt
    53341 ttcaattaaa aaagtgacac ttaactcaat atcaccgtag atctgagtgt cccagcacat
    53401 attagccttt gcgaccatct catccggatt ttttgcgcat gttttgagtt tattgaataa
    53461 ttccaggcta ttgataaact tttctctctc attctcgtcg atagccccag taaagtattc
    53521 tgaggcgttg atgttgccaa ccttgttatt cacgaaattg aacagaagtg tataagcctc
    53581 aggctgttta tctgaccaga acagatgacg taacgtctcc cacaagcgtt gaaagatatt
    53641 catcttcacg tctatcttgt ccccgctcac catcttataa atatcgttat tgctcatatt
    53701 taaaagtgta gccggcatgc ctggaactga tactttcatg gtaatagcct cgcattgagt
    53761 taaaaactca tcatgcgcac tctgaataaa tatactttca aaaatcgctc attcagatat
    53821 gtgtttttta tttttaagac gtaaaaatat aattccgctc gtcgtaaagc tctcaacctt
    53881 aagcagggtc aacgcgataa aaccaacaga acccctcccg taaaccgtgc attgagcctg
    53941 tccaccattc tgagtaaaca ccttttctca aagaagtgac cgccagggcg gccaccttta
    54001 atcgtgggca gttactcagc atcatttaca gggtcattta aaccctttct gctgcttaat
    54061 cagctcatag gcctgctgaa tttcctgcgc tttctgcttc gccatctcca tcatctcagg
    54121 cggcaaacct ttcgccacca acttatcggg atggtgttca ctcatcagct tacggtaggc
    54181 acgtttgatg gtggtcgcat catccgtcgg cttcacgccc agcacattac aggcatcttc
    54241 cagcgtcggg ccacgctgcg cttgctgcca gttaccaccg ccagattgct gctgataacc
    54301 gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact gggcgcggga
    54361 gatccctaat tcttcagcaa tgacatacag caccgcccgt tcattcgggt gcagtgaacc
    54421 atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt caaaacgacc
    54481 aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattgt ctgattttcc
    54541 cacccggaac gcgttttgcg ccgcagtacg ggaagcgcca tgcaggttca ttcggtccat
    54601 caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt tggttaaatg
    54661 ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa
    54721 ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca ggcctaacac
    54781 tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga tttttcccca
    54841 atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc acaatataaa
    54901 ggtgctttta ccgttttccg gctgcggtca attgtgggac atcgcctata atttgcatta
    54961 tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac aggattaaca
    55021 ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca acgttaccga
    55081 tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg gccaccatga
    55141 ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca cagtgcatgt
    55201 tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac ttacccgtga
    55261 ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt actggcagcg
    55321 tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc catcaaaaag
    55381 aggcaccagg acaaccggag ccggtacgta ccgttgatgc gctcggtaat gtccattacg
    55441 acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac accaaagata
    55501 ccaatgtctg ggaaggtgat taccagatgg tgggtcgtca gggccgcggt aaagcggacc
    55561 tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc tttacctcct
    55621 gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat gaccgcgaag
    55681 aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccggtaccg atcttttata
    55741 gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg
    55801 ctaagtacac cactaccaac tactttgagt tctaccttcc atattactgg aacatcgcgc
    55861 caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaat atcatgtggg
    55921 agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg gactatctgc
    55981 cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt cgttggttat
    56041 tctactggaa ccactccggg gtcatggatc aggtgtggcg tttcaacgtc gactacacca
    56101 aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc agtactgacg
    56161 gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat gccaccgttt
    56221 caaccaagca gttccaggtc ttcagcgagc agaacaccag tagctactcg gcagagccgc
    56281 agttagacgt taactactac cagaatgatg ttggtccgtt tgatacgcgt atttacggcc
    56341 aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt gttcacctgg
    56401 aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc gaagcgaagt
    56461 tgctggcaac ccattatcag caaactaatc ttgactggta taactccaga aacacaacca
    56521 agctggacga atccgttaac cgcgtaatgc cgcaatttaa agttgacggc aaaatggtct
    56581 tcgaacgtga tatggaaatg ctggctccgg gttataccca aacgctggaa ccgcgcgcgc
    56641 agtatttata cgtgccgtat cgcgatcaga gcgacatcta taactacgac tcgtctctgc
    56701 tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt gaccgtattg
    56761 cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat gctgccgttg
    56821 aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc actggcgatg
    56881 acaacataac atgggagaat gacgacaaaa cgggctcact ggtgtgggca ggcgatactt
    56941 actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat acacgtctgg
    57001 ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac cgtctggtac
    57061 agctgaacta ccgttacgcc agcccggaat atattcaggc tacgctgcct aagtactatt
    57121 ccacggctga gcaatataag aatggtattt cgcaggtagg tgccgtcgcc agctggccaa
    57181 ttgccgatcg ttggtcaatt gttggggcct actactacga caccaatgcg aacaagcaag
    57241 ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc gtcggttacg
    57301 agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac aacgcaatcg
    57361 gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg caagagatgc
    57421 tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc
    57481 cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct gcttctcggt
    57541 atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa agtcgcagcc
    57601 gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca gtcggtaaaa
    57661 ctgaacgctg ctcaggcaag gcagcaactt cctgatgacg cgacgctgcg ccaccaaatc
    57721 atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa
    57781 atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa caacatgacg
    57841 ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac ctatcgtaac
    57901 cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc
    57961 accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc
    58021 agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc gacttctgat
    58081 caagtgaacg aagcggaaag ccaggcgcgt gccattgtcg atcaggcgcg taacggcgct
    58141 gatttcggta agctggcgat tgctcattct gccgaccagc aggcactgaa cggcggccag
    58201 atgggctggg gccgtattca ggagttgccg gggatcttcg cccaggcatt aagcaccgcg
    58261 aagaaaggcg acattgttgg cccaattcgt tccggcgttg gcttccatat tctgaaagtt
    58321 aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc tcgccatatt
    58381 ctgctgaaac cgtcgccgat catgactgac gaacaggccc gcgtgaaact ggaacagatt
    58441 gctgccgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga gttctctcag
    58501 gatccaggct ctgctaacca gggcggtgat ctcggctggg ctacaccaga tattttcgat
    58561 ccggccttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc accggttcac
    58621 tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga taaaaccgac
    58681 gcggcacaga aagatcgtgc gtaccgcatg ctgatgaacc gtaagttctc ggaagaagca
    58741 gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag caactaatgg
    58801 ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc ccggacttag
    58861 ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc gatgccactc
    58921 tccttaccaa ccgggcagcg atgctcggtt tgccgctcac cctccgccct tattccccca
    58981 actcccctgc acaaccgcaa actgcgggca cattaacgct acttcctgtc gcgctacgtg
    59041 aatctgtcac tgcggggcag ttagcggttg aaaatgggca ttatgtggtg gaaacgctgg
    59101 cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc
    59161 ataaaggcgt cattaacgac gcaggcattc cgtttaccgg tcataccgag tttttcgaag
    59221 agcgttcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc
    59281 tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct gcgcttttgc
    59341 acgaagtgat tgctattttg catcacgatt tgcggaccaa atttggtatt gccgaaccgc
    59401 gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg ggtacggaag
    59461 agatagacac cattattccg gtgctcgacg agctgcgggc gcaggggatg aaactcaacg
    59521 ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc
    59581 tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc gggcgcggtg
    59641 tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc accgcgcttg
    59701 aactggcggg acgtggcaaa gccgatgtcg gcagttttat tacggcgctt aatctcgcca
    59761 tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact tagcccgtaa
    59821 acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg tttctgccat
    59881 taacccgcag aagggccagg cgatggtcga aatcggcccc ggtctggcgg cattgaccga
    59941 accggtcggc gaacgtctgg ataagctcac ggtcatcgaa cttgaccgcg atctggcggc
    60001 acgtctgcaa acgcatccat tcttaggccc gaaactgact atttatcagc aggatgcgat
    60061 gacctttaac tttggtgaac tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa
    60121 cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata ctgatgccat
    60181 tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa
    60241 cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg tgatcccagt
    60301 gctggaagta ccgccgtcag cctttacacc accacccaaa gtggattccg ccgtcgtgcg
    60361 cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgcgtgt tgagccgcat
    60421 caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt
    60481 tagcgtcgag gtgttaaccg gaatggggat cgacccggcg atgcgagcgg aaaatatctc
    60541 tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt tgcaggagag
    60601 ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta cattgaggca
    60661 caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat acgcaatctg
    60721 gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg caatggccgt
    60781 gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa
    60841 gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac
    60901 tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt attccgactc
    60961 gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg cgacgttcat
    61021 ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc tgggaaagat
    61081 accctctggc tgacgggcga tctggtcgcg cgcggcccgg gttcgctgga tgttctgcgc
    61141 tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg
    61201 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg
    61261 gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccagcctct gctgcaaatc
    61321 gacgaagaga aaaagctggt gatggcccac gccgggatca cgccacagtg ggatctgaag
    61381 accgccaaag agtgcgcgcg cgatgtagaa gcggtgctgt cgagtgactc ctatcccttc
    61441 tttcttgatg ccatgtacgg cgatatgcca aataactggt caccggaatt gcgggggctg
    61501 ggaagactgc ggtttatcac caacgccttt acccgtatgc gtttttgctt cccgaacggt
    61561 caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact gaaaccgtgg
    61621 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg
    61681 ctggaaggca aaggtacgcc ggaaggtatt tacgcgctgg atactggctg ctgctggggt
    61741 ggtacattaa cctgcctgcg ctgggaagat aaacactatt ttgtccagcc gtcgaaccgg
    61801 cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatacagg aaggccggat
    61861 aagacgcgac cggcgtcgca tccggcacta gccgtaaatt ctatacaaac ttaccgccgc
    61921 tccagaatct caaagcaata gctgtgagag ttctgcgcat cagcgtcgtg gaattcgctg
    61981 aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct
    62041 gcgtcgatat gcgtcagata cagtttttgc gcttttggca ggaactgctc ataaacgcga
    62101 ccaccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcatccacc
    62161 gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat aatatttttg
    62221 cgtcctggca gcggacgacc gatggattcc caggtatggc ggcccataat tacgggttta
    62281 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg catggcgttt
    62341 tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat tgagatttcc
    62401 cgataaaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac tggtgcgagg
    62461 atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctccca ctggactcgc
    62521 cgcattacaa tgaaataaaa acaattagtt agctgtaaag tgtgatttac gtcactcttt
    62581 attaggatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt ttcccttctt
    62641 cggttccctg ccagccatgt cgttgaatta atgacagatg ttcgcggtct tcggtaatga
    62701 tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg gtgtcgttct
    62761 caaccattgc catctcttct accatctgaa tattaaagcg gcggaacaca tcggcacgtt
    62821 ctcgcgcttc atacggcccc agacctaaac tttccagtgc cagacgcccg gttttcagcg
    62881 caccttcgaa ggtttcacgc tccggctttt caacgcctgc ctgacgcaaa cgaatgtagt
    62941 ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaaatgttct ttcaccatct
    63001 ccgtcagttg caggttggtt tgcggatcgt cgatggcgtt aatcagcact tccgctttcg
    63061 ccgctccggc agattccagt aaatccatcc gcgtggcatc gccataaaac actttcatac
    63121 caaatttacg caaggtttcg atatggtccg gatcgtgatc gagcaccacc attttcaccc
    63181 cgctcgagag cagtaaacgt ccggtgatct gcccaaaacg accgaatccg gcaatgatta
    63241 cccgcggctg ttcttcgtcg atctcatcgg cttcacgcgc ctcttcacta gaagattgct
    63301 caaggcgatt gaggatcacc agcagaatcg gcgttgccgc catcgacagc gccaccgcca
    63361 gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc gcaccaaata
    63421 ccacaaaggc aaactcacta ccctgcccta acaacaccgc aaaccagcga cgctgtttat
    63481 ttggcacctg caacggtcgg gcaatcagcc acagcatggc gattttgatg atgaggaaac
    63541 cgagcagcag aatgacaata cgcaatgggt tttcaatcag cgtgccaaag tctatcgaca
    63601 tgccaacgcc aatgaaaaac agccccagca gcaaaccttt aaagggttcg atatcgcttt
    63661 ccagcgcatg acgatattcc gagcttgcca gcagtacgcc cgccagaaac gcgcccatcg
    63721 ccatcgacaa gccaacctct tccagcagca aaccaaagcc aaacacgagg aataacgcca
    63781 cggcactaaa cacttcccgc aagccagagc gggcgacaaa gcgcagcgcc ggacgcgtga
    63841 catagcgccc cagcagtacc accagcacca gcgcacccgc cacttttaac gccgagagag
    63901 caaatgcgcc catcgtcgtc gaggcactgc tcgccgccag tagcgggatc atcgccacca
    63961 gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcga cccatttgcg
    64021 tcatcatcag attgcgttca ttcatcgcct gcatggcaat cgccgtagag gagagcgcca
    64081 gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc aagcaacata cagaacagcc
    64141 ccagcaggcc gccgcaaatc accatctgca atgcgccacc accgaacacc gccgcacgca
    64201 gcttccacag cctttgtgga tcgagttcga ggccgataat aaacagcatc agcaccacgc
    64261 caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc ccccacgggc
    64321 caataatgca gccagcgatc aggtagccaa gtaccgatcc cagaccaaga cgtaccgcaa
    64381 tgggtacaat cagcgccgcc gaaccgagat aaatcagcgc ctgaatcagc gtatggctat
    64441 ccatgatgtg cctcctgcca ttccagcaga cgttgcttat agtgccgcgc ctgcccttcg
    64501 agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag ccagttcagc
    64561 ccgcagtaga ttgccgtcgc ctgtagcggc tgtgacagca catcaaagcc cggatgcgca
    64621 ccaatctcaa aatggctttc tccgccgccg gtcgtcaccg cccacagcaa atgtttgcca
    64681 tgcagcgccg taccgccgtg accgtaagcc cagccgtgcg agaaaacttt atcgatccaa
    64741 agtttgagga gcggtggaat gctgtaccac tgcatcgggt gctgccagac gatcagatcg
    64801 gcgcgagaca gcgcctcctg ctcggcggca atatcgatat tgaagtcagg atagagttga
    64861 taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat ccgtttattc
    64921 gcatgggaat gatgcggata cggatgcgca taaattataa gaatcatagg tagcccgcgt
    64981 gattacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata
    65041 atattgattc agaaaagaga gaaatctgaa caaattatcc cattctctgc ccgcggatcg
    65101 tcatattgct ggtctgcgcc agtgacatcc ctcgagtctc cggggcaaac gctacggaaa
    65161 tcaacaagcc aaacagcgag atacccgccc ccattaacat cgtgttactg ataccgtaat
    65221 tattgataaa gatcggcagt gcccaggtcg aaacaatagt gccaatacgg cttaaggaca
    65281 taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagctcatta ggatagagcc
    65341 actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaatgcc atcactacca
    65401 gccagatccc catatccggg atcagcccca aaaccgccag cgccagcgtc atcatggcaa
    65461 agctgccaat caacaatgga cgccgtccgg cagtgtttaa ccacagcatc ggtggaatac
    65521 agccgagcat aaagaacagg ctaatcacca cgttccccag tgccgcgttt ttgccaacac
    65581 ccaatcccaa caaaccaacg atttgcgggc caaaggtgta gatggcgaac atcgggatca
    65641 cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca
    65701 gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt tcgccaaaca
    65761 gtttgatcat catctcttcg cactctttta ctcgcccttt gcgtaacaac cagcggggag
    65821 attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgct gcgctaccca
    65881 gcatccagcg ccaaccacct tccacatcat aaagccagta accgaccaga tcggcgcagg
    65941 tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc tgacgggtac
    66001 tggagaactc agtgatcatc gaagtggcga tgggataatc tgcaccgatg acaatgccga
    66061 tgagtacccg catcactaac agttcgacgg gggatgagac aaacatcgtc gccaccgata
    66121 tcacgccgat ggcgatgata tcgatgagga acattttgcg ccgtccgact ttatcggaga
    66181 tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc
    66241 caatccagtc agcgtccagt ttcagcgccg gcgtcagttg ctccagcgcc acgccaatca
    66301 ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa atgcggcggt
    66361 gaatagaaga gaatttaaga tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt
    66421 tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc gtaacggaac
    66481 tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc cagccccaga
    66541 atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc
    66601 tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcgc ctgtttatcg
    66661 gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt attgacgccc
    66721 agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct
    66781 tgatcagatt gatgaagccc actttcttac cgtggcggag gattttcttg cgcatcagtt
    66841 ccggcacgct gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc agttccgggt
    66901 agccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac atacgcatat
    66961 cgcgcagcgg gccactctca agatgctgac gatattccgc cagtttttgc ttactgaaat
    67021 cgtcgctttt catcgctgaa agcacggttt ttgctgcggc ttccccggcg gcaatcgcca
    67081 gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca tcaccggcaa
    67141 tcagtacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc ggcactacgt
    67201 gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc ggatgttgtt
    67261 tgaaatcttc cagcatttgc ggcaccgatt tttttgcgtc atgcagatga tggagaccac
    67321 aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc
    67381 catcggtggg ggaacccgca aacaggcaag ccgcaccctg attaccctgc aactgaaaac
    67441 ggtcttcgat aaccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg
    67501 tcggtttgac gcgttttgcc atccccaact tttcggcaag gatggagttc actccatcag
    67561 caaggatcac cgttttcgct tcaatcacat caccatcggc ttctacgccg acaactttgc
    67621 catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg cccgcttctt
    67681 cggcctgctc catcagccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg
    67741 atggcgaggt ttcgtcgcca ttgcagtagt ccatagtcat cgctgacttt tccgtcataa
    67801 acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggattcggcg aaacctggaa
    67861 taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg gcacctgcgg
    67921 aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc agtgcggcaa
    67981 ccgaaccggc aagcccagca ccgacgatga tggcgtcaaa gatatcttcg gacataagag
    68041 ctccctgtca gcggcagggc cgtaatgcag ccctgccaga gtggatcaac gcgctaaagc
    68101 tgcggtcagt gccggaagga tcttcacggc gtcgccaaca ataccgtaat ccgcgtactg
    68161 gaagatcggc gcatttttat ctttgttgat ggcgaaaatg gtttgcgacg cattagcacc
    68221 aaccatgtgc tggatctgcc cggagatccc caccgccagg tacagttcag gtttcagcat
    68281 caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt ccgccaccgg
    68341 acgagaacag gccaactccg cacctatcgc cttacaaagc tgttctgcca gcgcaatgtt
    68401 atctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac
    68461 gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca
    68521 ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat caaacgtgcc
    68581 gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc
    68641 gccgtaaacc atgtgtttca ctgtcgcttt accgtcctgt acgctgacgg tgctggcatc
    68701 gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg
    68761 agtattcggc aacagcacca gaccgtctgc tccgtgctgg cgaatagtgt cagccatgac
    68821 accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt
    68881 agcgcctagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg cattgatttg
    68941 attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaagggg tatcgctgaa
    69001 tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga
    69061 ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgc
    69121 tcgcgctgtt tcggcgcggc aacctgttgt tctgaccagg ctgcctctgc gttaaaaccg
    69181 atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat ggctttcatc
    69241 gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg cagcggaatg
    69301 cttaaggttt ctgtttcatc ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg
    69361 gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc taccagcaaa
    69421 ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcaa atcaaagcct
    69481 gctttctggg cggcagcagc cagtgcggtc gctgtttgtt gcggcagtgc ccgctcgaac
    69541 tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc tttacgccct
    69601 ttggcgttgg tcagggcttt accgcccaca cttagggccg tcacctgcgc ctctgctgcc
    69661 tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct tattttggca
    69721 tcggctttac tgaagtctaa cgaaccatca gcattattga ccgcaatatc ctgttcatca
    69781 ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat gaaggaaacg
    69841 tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga aataaaactt
    69901 taatcaccaa tattgaaaat gtcacacgca gataaatcca actttcaata ttgttaagtc
    69961 cctcaccaat attgaaaaca tggcgtagca aaaagaaatt ttcaatattg ctttatggat
    70021 caccaatatt gaaagtcaga tgctattttt ttactcacaa tcaaactcat cacattgata
    70081 aacaatgaat atttacacaa agacgccctg tttttacccg ataaaaaact gtgactaaca
    70141 tcacagaacg cagccaattg aatacccatt atgagttagc cattaacgcg tccacgaggt
    70201 taataataat tatattaaat gttaacaaaa ataaaacaaa cgggaacgca aaataaatat
    70261 tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg aatagaaccg
    70321 aaggttttct ttccgccgtt aataatcgtc ggcatacttt gttggcttac agtcagagat
    70381 ctggatgcgg cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa tgtatgggga
    70441 tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct ggtgtttggc
    70501 ccgtatgcca aaaagcgttt aggtaacgaa ccaccagaat ttagtaccgc cagttggatc
    70561 tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc gattgagatc
    70621 tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg ggcgaaagag
    70681 ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac ttatagcttc
    70741 ctttctgtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat tcgccccagc
    70801 tccaccctgg tgccgctggt gggtgaaaaa cacgccaaag ggttgttcgg taccatcgtc
    70861 gacaacttct atctcgtcgc cttgattttc gcgatgggta ccagtctggg ccttgccacg
    70921 ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct ccaactggac
    70981 gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcctg cggtctgcaa
    71041 aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat gctgggttgg
    71101 gtgttcattg tcagcggtgc cagcttcatc atgaactact tcaccgattc ggtggggatg
    71161 ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa aggcggcttc
    71221 ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgctat ccagatgagt
    71281 atcttcctcg cccgcatctc ccgtggtcgt accgtgcgtg aactgtgctt cggcatggtg
    71341 ctggggctga cagcgtcaac ctggatcctg tggactgtac tcggtagtaa cactctgctg
    71401 ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg tgtggcgcgc
    71461 gccatcattg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg gggcttcttc
    71521 atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac cctggcgatg
    71581 tccacttgcc gcgaagtacg cgatggtgaa gaaccaccgc tactggtgcg tatcggctgg
    71641 tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct gaaaccgatt
    71701 caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat ggtgacgctc
    71761 tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa aatatcaaga
    71821 ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc cggtatccgc
    71881 gaacttatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg tgacagcgtc
    71941 tacccggaac gttttgtcaa agcactggcg gatatgggga tcgacagtct gctgatccct
    72001 gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg gatggagctg
    72061 ggacgtctgg gggcaccaac ctatgtactg taccagttgc cgggcgggtt caacaccttc
    72121 ctgcgcgaag gcacacaaga gcagatcgac aagattatgg ctttccgcgg caccggtaag
    72181 cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg tagcctgaaa
    72241 acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg ttttattacc
    72301 agcagcgcct acaccccgta catcgttgtg atggcgcgcg acggggcttc tccggacaaa
    72361 cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt gaccaaactt
    72421 gaaaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga cgtggaactg
    72481 gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa agaagagttc
    72541 gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg cgcctttgaa
    72601 gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg tcgtttccag
    72661 ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa aaacatgctg
    72721 tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc agcgatgtgc
    72781 aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca ggtgctgggc
    72841 ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgatct gcgtgtagac
    72901 cgcgtctccg ggggatctga cgaaatgcag atcctgacgc tgggtcgcgc ggtgctgaag
    72961 caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat cagacctacg
    73021 ggagcacgaa tgtaggccgg ataaagcgtt tacgccgcat ccggcagtca tgcgctacat
    73081 aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcgga ccgctggccg
    73141 gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccggg caaatgttcg
    73201 cagaatgggg tgcggaagtt atctggatcg agaacgtcgc ctgggccgac accattcgcg
    73261 ttcaaccgaa ctacccgcag ctctcccgcc gcaatttgca cgcgctgtcg ttaaatattt
    73321 tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat atcttcatcg
    73381 aagccagcaa aggtccggcc tttgcccgtc gtggcattac cgatgaagtc ctgtggcagc
    73441 acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc accgaggagt
    73501 acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac ctgattcaga
    73561 acggtgatgt tgaccagcca atgcctgctt tcccgtatac cgccgattac ttttctggcc
    73621 tgaccgccac cacggcggcg ctggcagcac tgcataaagc gcgtgaaacc ggtaaaggcg
    73681 aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac ttcatgatgg
    73741 attacttcaa tggcggcgaa atgtgcccgc gcatgaccaa aggtaaagat ccctactacg
    73801 ccggttgcgg tctgtacaaa tgcgccgacg gctacatcgt gatggaactg gtgggcatta
    73861 ctcaaattga agagtgcttt aaagatattg gcctcgcgca cctgcttggt acgccggaaa
    73921 tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca ctggtagaag
    73981 agaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa cgctttgccg
    74041 aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc aatccacaat
    74101 atgtggctcg cgaatcaatc actcagtggc aaacgatgga tggtcgcacc tgcaaagggc
    74161 cgaacgtgat gcctaaattc aaaaataacc ccggacaaat ctggcgcgga atgccctcac
    74221 atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac gacattcagg
    74281 agttggtcag taaaggtctg gccaaagttg aggactaaac gcgtggcgtc agtcggcttg
    74341 cctgactagc gcccgcccaa taacaaacga acaacaatgg atagaggtgc aatggatatc
    74401 attggcggac aacatctacg tcaaatgtgg gacgatctgg cggacgttta cggtcataaa
    74461 acggcgctga tttgtgaatc cagcagcgga gtcgttaacc ggtatagtta tcttgagtta
    74521 aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg caaaggcgac
    74581 aaggttgctc tacatctcga caactgcccg gaatttatct tttgctggtt cgggctggca
    74641 aaaattggcg cgattatggt gccgattaac gcccgcctgt tacgcgagga aagcgcgtgg
    74701 atcctgcaaa atagccaggc gtgcctgctg gtgaccagtg cgcaatttta tcctatgtat
    74761 caacagattc agcaggaaga tgcctctcag ttgcggcaca tttgcctgat agatatggca
    74821 cttcccgctg atgatggcgt gagttcgttt actcaactga aaaatcaaca acctgccacc
    74881 ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt cacctccggc
    74941 accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt cgctggatat
    75001 tactccgcct ggcaatgtgc actgcgtgac gatgacgtct acctgacggt aatgcctgcg
    75061 tttcatattg actgccagtg tactgcggcg atggcggcgt tttctgccgg ggccaccttt
    75121 gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggtgcagaa gtaccgcgcc
    75181 accattaccg aatgtattcc gatgatgatt cgtacgttga tggtccagcc gccttcagcc
    75241 aacgatcggc aacactgcct gcgggaagtg atgttttatc tcaacctgtc ggagcaggaa
    75301 aaagacgcgt tttgtgaacg cttcagcgtt cgcttgctga cgtcttatgg gatgacggaa
    75361 accattgtgg gcattatcgg cgatcgccct ggcgataaac gacgctggcc gtcgattggt
    75421 cgggcgggct tttgctacga agcggagatc cgcgacgatc acaatcgtcc cctcccggct
    75481 ggtgagctcg gtgaaatctg tattaaaggc gtaccaggga aaaccatctt caaagagtat
    75541 tttctcaacc cgaaagccac tgcgaaagtg ctggaagccg atggctggct acataccggc
    75601 gataccggat accgcgacga agagggcttt ttttatttcg tcgatcgccg ctgcaatatg
    75661 atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg aaaatattat cgccgcgcac
    75721 ccgaaaattc aggacatcgt ggttgtgggt attaaagatt cgatacgcga tgaagccatc
    75781 aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt tttccgcttc
    75841 tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag aaaagatctg
    75901 ccacgtaatt gctcggggaa aataattaga aagaatctta aataaatgaa acggcaggga
    75961 actaccctgc cagctaataa tcacgctcta aaataatatg cattttttgc agagatgttg
    76021 tcgtccctga aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca tctgacccgc
    76081 aatggatcaa ttctggaaat tacccttgat cgcccaaaag cgaatgctat tgatgcaaaa
    76141 accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca attacgtgtc
    76201 gccattatta ccggtgccgg agagaaattc ttttccgcgg gctgggattt aaaagcggca
    76261 gcagaaggcg aagcaccgga tgctgacttt ggcccgggtg gttttgcggg attaaccgaa
    76321 attttcaatc tcgacaaacc agttatcgcc gctgtgaacg gctatgcctt tggcggcggc
    76381 tttgaactgg cactggcggc agattttatt gtctgtgccg ataacgccag cttcgccctg
    76441 ccggaagcca aactgggcat cgttcctgac agcggcggcg tgctgcgtct gccgaagatc
    76501 ctgcc
//
LOCUS       DAYPHN010000004        75472 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000004 DAYPHN010000000
VERSION     DAYPHN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.004"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1086)
                     /gene="icd"
                     /locus_tag="ACG0BW_000235"
     CDS             complement(<1..1086)
                     /gene="icd"
                     /locus_tag="ACG0BW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFK7257251.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEM"
     gene            1258..1911
                     /gene="rluE"
                     /locus_tag="ACG0BW_000236"
     CDS             1258..1911
                     /gene="rluE"
                     /locus_tag="ACG0BW_000236"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFK7257252.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKLENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            1921..2382
                     /gene="nudJ"
                     /locus_tag="ACG0BW_000237"
     CDS             1921..2382
                     /gene="nudJ"
                     /locus_tag="ACG0BW_000237"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFK7257253.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEANETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSRQRYPLEMIGDFNWPFTKGVI"
     gene            2436..3542
                     /gene="mnmA"
                     /locus_tag="ACG0BW_000238"
                     /gene_synonym="trmU"
     CDS             2436..3542
                     /gene="mnmA"
                     /locus_tag="ACG0BW_000238"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFK7257254.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3578..4219
                     /gene="hflD"
                     /locus_tag="ACG0BW_000239"
     CDS             3578..4219
                     /gene="hflD"
                     /locus_tag="ACG0BW_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFK7257255.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4223..5593
                     /gene="purB"
                     /locus_tag="ACG0BW_000240"
     CDS             4223..5593
                     /gene="purB"
                     /locus_tag="ACG0BW_000240"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFK7257256.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5762..6433
                     /gene="phoP"
                     /locus_tag="ACG0BW_000241"
     CDS             5762..6433
                     /gene="phoP"
                     /locus_tag="ACG0BW_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFK7257257.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6433..7893
                     /gene="phoQ"
                     /locus_tag="ACG0BW_000242"
     CDS             6433..7893
                     /gene="phoQ"
                     /locus_tag="ACG0BW_000242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFK7257258.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHTIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            7969..9090
                     /gene="roxA"
                     /locus_tag="ACG0BW_000243"
                     /gene_synonym="ycfD"
     CDS             7969..9090
                     /gene="roxA"
                     /locus_tag="ACG0BW_000243"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFK7257259.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVSPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9139..10365)
                     /gene="pepT"
                     /locus_tag="ACG0BW_000244"
     CDS             complement(9139..10365)
                     /gene="pepT"
                     /locus_tag="ACG0BW_000244"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFK7257260.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10615..11751
                     /gene="potA"
                     /locus_tag="ACG0BW_000245"
     CDS             10615..11751
                     /gene="potA"
                     /locus_tag="ACG0BW_000245"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFK7257261.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11735..12592
                     /gene="potB"
                     /locus_tag="ACG0BW_000246"
     CDS             11735..12592
                     /gene="potB"
                     /locus_tag="ACG0BW_000246"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFK7257262.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12589..13383
                     /gene="potC"
                     /locus_tag="ACG0BW_000247"
     CDS             12589..13383
                     /gene="potC"
                     /locus_tag="ACG0BW_000247"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFK7257263.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13380..14426
                     /gene="potD"
                     /locus_tag="ACG0BW_000248"
     CDS             13380..14426
                     /gene="potD"
                     /locus_tag="ACG0BW_000248"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFK7257264.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKQGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14484..14945
                     /gene="ymfA"
                     /locus_tag="ACG0BW_000249"
     CDS             14484..14945
                     /gene="ymfA"
                     /locus_tag="ACG0BW_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFK7257265.1"
                     /translation="MSQDSKVFFRIFLGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKSRRKRKSRP"
     gene            14942..15727
                     /locus_tag="ACG0BW_000250"
     CDS             14942..15727
                     /locus_tag="ACG0BW_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFK7257266.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPDVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNDQVESGLDDKHLGLLVENHTHAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTILGLFAWAVAFIVLTTFFNYTTRLNYALKFAVAMTVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(15865..16686)
                     /gene="cobB"
                     /locus_tag="ACG0BW_000251"
     CDS             complement(15865..16686)
                     /gene="cobB"
                     /locus_tag="ACG0BW_000251"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFK7257267.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRVFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16702..17613)
                     /gene="nagK"
                     /locus_tag="ACG0BW_000252"
     CDS             complement(16702..17613)
                     /gene="nagK"
                     /locus_tag="ACG0BW_000252"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFK7257268.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17642..18886)
                     /gene="lolE"
                     /locus_tag="ACG0BW_000253"
     CDS             complement(17642..18886)
                     /gene="lolE"
                     /locus_tag="ACG0BW_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFK7257269.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18886..19587)
                     /gene="lolD"
                     /locus_tag="ACG0BW_000254"
     CDS             complement(18886..19587)
                     /gene="lolD"
                     /locus_tag="ACG0BW_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFK7257270.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19580..20779)
                     /gene="lolC"
                     /locus_tag="ACG0BW_000255"
     CDS             complement(19580..20779)
                     /gene="lolC"
                     /locus_tag="ACG0BW_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFK7257271.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21040..22113
                     /gene="ycfT"
                     /locus_tag="ACG0BW_000256"
     CDS             21040..22113
                     /gene="ycfT"
                     /locus_tag="ACG0BW_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFK7257272.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLRGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22241..25687
                     /gene="mfd"
                     /locus_tag="ACG0BW_000257"
     CDS             22241..25687
                     /gene="mfd"
                     /locus_tag="ACG0BW_000257"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFK7257273.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGYAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQAYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25832..26794
                     /gene="ldtC"
                     /locus_tag="ACG0BW_000258"
     CDS             25832..26794
                     /gene="ldtC"
                     /locus_tag="ACG0BW_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFK7257274.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26877..27134)
                     /gene="bhsA"
                     /locus_tag="ACG0BW_000259"
                     /gene_synonym="ycfR"
     CDS             complement(26877..27134)
                     /gene="bhsA"
                     /locus_tag="ACG0BW_000259"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFK7257275.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27375..28007
                     /gene="comR"
                     /locus_tag="ACG0BW_000260"
     CDS             27375..28007
                     /gene="comR"
                     /locus_tag="ACG0BW_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFK7257276.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIGNTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIVRTTLRLWPELVK"
     gene            complement(28069..28608)
                     /gene="ycfJ"
                     /locus_tag="ACG0BW_000261"
     CDS             complement(28069..28608)
                     /gene="ycfJ"
                     /locus_tag="ACG0BW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFK7257277.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRQPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(28835..30139)
                     /gene="ndh"
                     /locus_tag="ACG0BW_000262"
     CDS             complement(28835..30139)
                     /gene="ndh"
                     /locus_tag="ACG0BW_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFK7257278.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(30539..31081)
                     /gene="ycfP"
                     /locus_tag="ACG0BW_000263"
     CDS             complement(30539..31081)
                     /gene="ycfP"
                     /locus_tag="ACG0BW_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFK7257279.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(31104..32129)
                     /gene="nagZ"
                     /locus_tag="ACG0BW_000264"
     CDS             complement(31104..32129)
                     /gene="nagZ"
                     /locus_tag="ACG0BW_000264"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFK7257280.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(32140..32964)
                     /gene="thiK"
                     /locus_tag="ACG0BW_000265"
     CDS             complement(32140..32964)
                     /gene="thiK"
                     /locus_tag="ACG0BW_000265"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFK7257281.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(32945..33586)
                     /gene="lpoB"
                     /locus_tag="ACG0BW_000266"
     CDS             complement(32945..33586)
                     /gene="lpoB"
                     /locus_tag="ACG0BW_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFK7257282.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(33600..33977)
                     /gene="ycfL"
                     /locus_tag="ACG0BW_000267"
     CDS             complement(33600..33977)
                     /gene="ycfL"
                     /locus_tag="ACG0BW_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFK7257283.1"
                     /translation="MRKGCYGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(33980..34339)
                     /gene="hinT"
                     /locus_tag="ACG0BW_000268"
     CDS             complement(33980..34339)
                     /gene="hinT"
                     /locus_tag="ACG0BW_000268"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFK7257284.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34673..36862
                     /gene="fhuE"
                     /locus_tag="ACG0BW_000269"
     CDS             34673..36862
                     /gene="fhuE"
                     /locus_tag="ACG0BW_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFK7257285.1"
                     /translation="MLSTQFNRDNQHQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(36922..38355)
                     /gene="ptsG"
                     /locus_tag="ACG0BW_000270"
     CDS             complement(36922..38355)
                     /gene="ptsG"
                     /locus_tag="ACG0BW_000270"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFK7257286.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38650..39447)
                     /gene="ycfH"
                     /locus_tag="ACG0BW_000271"
     CDS             complement(38650..39447)
                     /gene="ycfH"
                     /locus_tag="ACG0BW_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFK7257287.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(39458..40462)
                     /gene="holB"
                     /locus_tag="ACG0BW_000272"
     CDS             complement(39458..40462)
                     /gene="holB"
                     /locus_tag="ACG0BW_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFK7257288.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(40459..41100)
                     /gene="tmk"
                     /locus_tag="ACG0BW_000273"
     CDS             complement(40459..41100)
                     /gene="tmk"
                     /locus_tag="ACG0BW_000273"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFK7257289.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(41090..42112)
                     /gene="yceG"
                     /locus_tag="ACG0BW_000274"
     CDS             complement(41090..42112)
                     /gene="yceG"
                     /locus_tag="ACG0BW_000274"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFK7257290.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(42115..42924)
                     /gene="pabC"
                     /locus_tag="ACG0BW_000275"
     CDS             complement(42115..42924)
                     /gene="pabC"
                     /locus_tag="ACG0BW_000275"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFK7257291.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(43044..44285)
                     /gene="fabF"
                     /locus_tag="ACG0BW_000276"
     CDS             complement(43044..44285)
                     /gene="fabF"
                     /locus_tag="ACG0BW_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFK7257292.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(44373..44609)
                     /gene="acpP"
                     /locus_tag="ACG0BW_000277"
     CDS             complement(44373..44609)
                     /gene="acpP"
                     /locus_tag="ACG0BW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFK7257293.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(44820..45554)
                     /gene="fabG"
                     /locus_tag="ACG0BW_000278"
     CDS             complement(44820..45554)
                     /gene="fabG"
                     /locus_tag="ACG0BW_000278"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFK7257294.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(45567..46496)
                     /gene="fabD"
                     /locus_tag="ACG0BW_000279"
     CDS             complement(45567..46496)
                     /gene="fabD"
                     /locus_tag="ACG0BW_000279"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFK7257295.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(46512..47465)
                     /gene="fabH"
                     /locus_tag="ACG0BW_000280"
     CDS             complement(46512..47465)
                     /gene="fabH"
                     /locus_tag="ACG0BW_000280"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFK7257296.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(47533..48603)
                     /gene="plsX"
                     /locus_tag="ACG0BW_000281"
     CDS             complement(47533..48603)
                     /gene="plsX"
                     /locus_tag="ACG0BW_000281"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFK7257297.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48684..48857)
                     /gene="rpmF"
                     /locus_tag="ACG0BW_000282"
     CDS             complement(48684..48857)
                     /gene="rpmF"
                     /locus_tag="ACG0BW_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFK7257298.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(48909..49430)
                     /gene="yceD"
                     /locus_tag="ACG0BW_000283"
     CDS             complement(48909..49430)
                     /gene="yceD"
                     /locus_tag="ACG0BW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFK7257299.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            49629..50213
                     /gene="yceF"
                     /locus_tag="ACG0BW_000284"
     CDS             49629..50213
                     /gene="yceF"
                     /locus_tag="ACG0BW_000284"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFK7257300.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(50325..51284)
                     /gene="rluC"
                     /locus_tag="ACG0BW_000285"
     CDS             complement(50325..51284)
                     /gene="rluC"
                     /locus_tag="ACG0BW_000285"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFK7257301.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            51857..55042
                     /gene="rne"
                     /locus_tag="ACG0BW_000286"
     CDS             51857..55042
                     /gene="rne"
                     /locus_tag="ACG0BW_000286"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFK7257302.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPTAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQAAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVKPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            55175..55468
                     /locus_tag="ACG0BW_000287"
     CDS             55175..55468
                     /locus_tag="ACG0BW_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFK7257303.1"
                     /translation="MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            55465..55959
                     /locus_tag="ACG0BW_000288"
     CDS             55465..55959
                     /locus_tag="ACG0BW_000288"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7257304.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(56054..57007)
                     /gene="flgL"
                     /locus_tag="ACG0BW_000289"
     CDS             complement(56054..57007)
                     /gene="flgL"
                     /locus_tag="ACG0BW_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFK7257305.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(57019..58662)
                     /gene="flgK"
                     /locus_tag="ACG0BW_000290"
     CDS             complement(57019..58662)
                     /gene="flgK"
                     /locus_tag="ACG0BW_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFK7257306.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMTQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTLTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(58728..59669)
                     /gene="flgJ"
                     /locus_tag="ACG0BW_000291"
     CDS             complement(58728..59669)
                     /gene="flgJ"
                     /locus_tag="ACG0BW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFK7257307.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASDNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(59669..60766)
                     /gene="flgI"
                     /locus_tag="ACG0BW_000292"
     CDS             complement(59669..60766)
                     /gene="flgI"
                     /locus_tag="ACG0BW_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFK7257308.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQMQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(60778..61476)
                     /gene="flgH"
                     /locus_tag="ACG0BW_000293"
     CDS             complement(60778..61476)
                     /gene="flgH"
                     /locus_tag="ACG0BW_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFK7257309.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(61529..62311)
                     /gene="flgG"
                     /locus_tag="ACG0BW_000294"
     CDS             complement(61529..62311)
                     /gene="flgG"
                     /locus_tag="ACG0BW_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFK7257310.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(62448..63203)
                     /gene="flgF"
                     /locus_tag="ACG0BW_000295"
     CDS             complement(62448..63203)
                     /gene="flgF"
                     /locus_tag="ACG0BW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFK7257311.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(63223..64428)
                     /gene="flgE"
                     /locus_tag="ACG0BW_000296"
     CDS             complement(63223..64428)
                     /gene="flgE"
                     /locus_tag="ACG0BW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFK7257312.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTADHAMTLVFNANGVLTSNPTANITTGAINGADPATFSLSFLNSMQQNTGANNIVV
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(64453..65148)
                     /gene="flgD"
                     /locus_tag="ACG0BW_000297"
     CDS             complement(64453..65148)
                     /gene="flgD"
                     /locus_tag="ACG0BW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFK7257313.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(65160..65564)
                     /gene="flgC"
                     /locus_tag="ACG0BW_000298"
     CDS             complement(65160..65564)
                     /gene="flgC"
                     /locus_tag="ACG0BW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFK7257314.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(65568..65984)
                     /gene="flgB"
                     /locus_tag="ACG0BW_000299"
     CDS             complement(65568..65984)
                     /gene="flgB"
                     /locus_tag="ACG0BW_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFK7257315.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            66139..66798
                     /gene="flgA"
                     /locus_tag="ACG0BW_000300"
     CDS             66139..66798
                     /gene="flgA"
                     /locus_tag="ACG0BW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFK7257316.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLASQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            66874..67167
                     /gene="flgM"
                     /locus_tag="ACG0BW_000301"
     CDS             66874..67167
                     /gene="flgM"
                     /locus_tag="ACG0BW_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFK7257317.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            67172..67588
                     /gene="flgN"
                     /locus_tag="ACG0BW_000302"
     CDS             67172..67588
                     /gene="flgN"
                     /locus_tag="ACG0BW_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFK7257318.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNSANSVDISQRWQAITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(67628..69163)
                     /gene="murJ"
                     /locus_tag="ACG0BW_000303"
     CDS             complement(67628..69163)
                     /gene="murJ"
                     /locus_tag="ACG0BW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFK7257319.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(69273..70196)
                     /gene="yceM"
                     /locus_tag="ACG0BW_000304"
     CDS             complement(69273..70196)
                     /gene="yceM"
                     /locus_tag="ACG0BW_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFK7257320.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(70198..70845)
                     /gene="yceH"
                     /locus_tag="ACG0BW_000305"
     CDS             complement(70198..70845)
                     /gene="yceH"
                     /locus_tag="ACG0BW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFK7257321.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(70856..71440)
                     /gene="rimJ"
                     /locus_tag="ACG0BW_000306"
     CDS             complement(70856..71440)
                     /gene="rimJ"
                     /locus_tag="ACG0BW_000306"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFK7257322.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            71676..72884
                     /gene="mdtH"
                     /locus_tag="ACG0BW_000307"
     CDS             71676..72884
                     /gene="mdtH"
                     /locus_tag="ACG0BW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFK7257323.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            72948..73595
                     /gene="grxB"
                     /locus_tag="ACG0BW_000308"
     CDS             72948..73595
                     /gene="grxB"
                     /locus_tag="ACG0BW_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFK7257324.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            73729..74289
                     /gene="yceB"
                     /locus_tag="ACG0BW_000309"
     CDS             73729..74289
                     /gene="yceB"
                     /locus_tag="ACG0BW_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFK7257325.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLHEDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            74395..75441
                     /gene="pyrC"
                     /locus_tag="ACG0BW_000310"
     CDS             74395..75441
                     /gene="pyrC"
                     /locus_tag="ACG0BW_000310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFK7257326.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFSPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRNGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        18255 a        20080 c        18955 g        18182 t
ORIGIN      
        1 catctcagcg gagagaataa tagagcctgg gtttactttg tcctgaccgg catatttcgg
       61 cgcagtaccg tgggtggctt caaacagggc gcattcgtca ccgatgtttg cgcctggggc
      121 gataccgata ccgccgacct gtgcagccag agcgtcagaa atgtagtcac cgttcaggtt
      181 catacaggcg ataacatcat attcagccgg acgcagcagg atctgttgca ggaatgcatc
      241 agcaatcaca tctttaatga cgatctcttt gccggtattc gggtttttaa ctttcagcca
      301 cgggccgccg tcgatcagtt caccgccaaa ctcttcacgc gccagctggt agccccagtc
      361 tttaaacgcg ccttcggtga acttcatgat gttgcctttg tgcaccaggg tcacagagtc
      421 acgatcgtta gcaattgcgt attcgatcgc tgcacgaacc agacgtttgg taccttcttc
      481 agaacacggc ttaataccga tgccgcaatg ttccgggaag cgaattttct tcacgcccat
      541 ctcttcacgc aggaatttaa tcactttctc agcgtcggca gagtctgctt tccattcgat
      601 acccgcataa atgtcttccg agttttcacg gaagataacc atatcggtca gttcagggtg
      661 tttaaccggg cttggggtgc cctgatagta acgtaccgga cgcaggcaga tgtagagatc
      721 cagttcctgg cgcagggcaa cgttcagaga acgaataccg ccaccaaccg gagtggtcag
      781 agggccttta atggcaacgc gatattcacg aatcagatca agggtttcag caggcagcca
      841 gacatcctga ccgtaaacct gtgtggattt ttcaccggtg taaatttcca tccaggagat
      901 tttacgctcg cctttatagg ctttctcgac tgcagcgtcg accactttca gcatggctgg
      961 ggttacatct acaccgattc catcaccttc aatgtaaggg ataatcggat tttcaggaac
     1021 gttgagtttg ccgttttgca gggtgatctt cttgccttgt gccggaacaa ctactttact
     1081 ttccattcac ctctccttcg agcgctactg gtttgctcgt ctgccaccac gttgcatatg
     1141 cgtttgcgtc ctgcgatacg gatgcattag agcaattttt tgttaatgat ttgtaattgg
     1201 cttgtcaata ctaccctatt gtttgccgtc atgaaagatg cgcgttatta ggctataatg
     1261 cggcaattca taatctctga aaataccatg caaaaaactt cttttagaaa tcaccaggtt
     1321 aagcgattca gctcgcaacg ttctaccagg cgtaaacttg aaaaccagcc cacgcgcgtg
     1381 atcctgttca ataaacccta cgatgttctt ccgcagttca ccgatgaagc cggacgcaaa
     1441 acattaaaag aattcatccc ggttcagggc gtttatgcag caggtcgtct tgaccgcgat
     1501 agcgaagggt tactggtgct gaccaataac ggcgcgttgc aggcgcgttt aacccagccg
     1561 ggtaaacgca ccggaaaaat ctattatgtg caggtggaag gtattcccac acaagacgca
     1621 cttgaagcct tgcgcaatgg cgtaacctta aatgatggcc ctaccctgcc cgcaggcgta
     1681 gagctggttg acgaacccgc gtggttatgg ccgcggaatc caccaattcg tgaacgcaaa
     1741 agtattccca ccagctggct gaagatcacc ttatatgaag gacgtaatcg ccaggtgcgc
     1801 cgcatgaccg cccatgttgg tttccccacg ctgcgactga ttcgctatgc gatgggtgat
     1861 tactctttgg ataatcttgc caacggcgaa tggcgagaag cgacagatta aggacgcaaa
     1921 atgtttaaac cgcacgttac cgttgcttgc gtggtgcacg cagaaggcaa atttttagtc
     1981 gttgaagaga cgattaatgg taaagcgtta tggaaccaac ctgccgggca tctggaagcc
     2041 aatgaaacct tagtggaagc cgccgcccgt gagctgtggg aagaaaccgg catcagcgcg
     2101 cagccgcaac actttattcg tatgcatcag tggattgcgc cagataaaac gccgtttttg
     2161 cgtttcctgt ttgctattga gcttgagcaa atatgcccga cccagcctca tgacagcgat
     2221 atcgactgct gccgttgggt tagcgccgaa gaaattttac aggcgtcaaa tctgcgttcg
     2281 ccgctggtgg cggaaagtat tcgttgttat caaagcaggc aacgctatcc gctagagatg
     2341 attggcgatt ttaactggcc ttttacaaag ggtgtcatct aaaaggctgc gtgatagaat
     2401 acgccgcctt gaagttcaat gtcgtgagtg atccaatgtc tgaaaccgca aaaaaagtaa
     2461 tcgtcggcat gtccggcggt gtcgattcct ccgtttctgc ctggctgttg caacaacagg
     2521 gatatcaggt cgaaggcctg tttatgaaga actgggaaga agacgacggt gaggaatatt
     2581 gcacagcggc agcggatctg gctgatgccc aggctgtctg cgacaagctc ggcattgaac
     2641 tgcacaccgt taactttgct gccgagtact gggacaacgt cttcgaactg ttccttgccg
     2701 aatataaagc cggtcgcacg ccgaatccgg atattctgtg caacaaagag atcaaattta
     2761 aagccttcct cgaatttgcc gctgaagatt taggtgccga ttatatcgct accggtcatt
     2821 acgtacgtcg ggcagatgtc gatggcaaga gccgcctgct gcgtggtctg gacagcaata
     2881 aagaccagag ctacttcctt tatacgctca gccatgagca gattgcgcaa agcctgttcc
     2941 cggtcggcga actggaaaaa ccgcaggtgc gtaagattgc tgaagatctc ggtctggtca
     3001 ccgcgaagaa aaaagactct accggcattt gcttcattgg cgagcgtaaa ttccgcgagt
     3061 tcctgggccg ttatctcccg gcgcaaccgg gcaaaatcat taccgtcgat ggcgatgaaa
     3121 ttggtgagca ccaggggctg atgtatcaca ccctcggtca gcgtaaaggt ctgggtatcg
     3181 gtggcaccaa agacggcacc gaagaaccgt ggtatgtggt cgataaagac gtcgaaaaca
     3241 acattctgat tgttgcgcag ggccatgaac acccgcgtct gatgtctgtc ggtctgattg
     3301 cccagcagct gcactgggtc gatcgcgagc cgttcaccgg tactatgcgt tgcacagtaa
     3361 aaacccgcta tcgccagacc gacatcccgt gcaccgtcaa ggcgctggac gatgatcgca
     3421 ttgaagtgat tttcgatgag ccggttgccg ccgtgacgcc gggccagtct gccgttttct
     3481 ataacggtga agtgtgcctc ggtggcggta ttattgagca gcgtctgccg ctgccggtct
     3541 gattattatc tttacttaaa acaaggaagc agtgaacgtg gcaaagaatt actatgacat
     3601 caccctcgcc ctggccggta tttgtcagtc ggcacgcctg gtgcaacaac tcgctcacca
     3661 ggggcattgt gatgccgatg cgctacacgt ctcactcaac agtattattg atatgaaccc
     3721 cagctcgacg ctggcggttt ttggcggtag cgaagccaac ctgcgcgtcg ggctggaaac
     3781 cttgctcggc gtgctcaatg ccagcagtcg ccagggctta aacgctgagt taacccgcta
     3841 cacactcagc ctgatggtgc ttgagcgcaa actctcctca gcgaaaggtg cgctcgacac
     3901 tctgggcaac cggatcaacg gcctgcaacg ccagctcgaa cacttcgatt tacagtccga
     3961 aacgctgatg agcgcgatgg ctgctatcta tgttgatgtg attagcccgc ttggcccgcg
     4021 cattcaggta accggttccc ctgctgtact gcaaagccca caagtgcagg cgaaagttcg
     4081 cgcaaccctg ctggcaggca ttcgcgccgc cgtgctctgg caccaggttg gcggcggacg
     4141 tctgcaactg atgttttctc gtaatcgcct gaccactcag gcaaaacaaa ttcttgctca
     4201 tttaaccccg gagttgtgat ctatggaatt atcctcactg accgccgttt cccctgtcga
     4261 tggacgctac ggcgataaag tcagcgcgct gcgcgggatt ttcagcgaat atggtttgct
     4321 gaaattccgt gtacaagttg aagtacgttg gctgcaaaaa ctggccgcgc acgcagcgat
     4381 caaggaagtt cctgcttttg ctgccgacgc aatcggttac cttgatgcaa ttgtcgccag
     4441 tttcagcgaa gaagatgcag cacgcatcaa aaccatcgag cgtaccacta accacgacgt
     4501 taaagcggtt gagtatttcc tgaaagaaaa agtggcggag atcccggaac tgcacgcggt
     4561 ttctgaattc atccactttg cctgtacttc ggaagatatc aataacctct cccacgcgtt
     4621 gatgctgaaa accgcgcgtg atgaagtgat cctgccgtac tggcgtcaac tgattgatgg
     4681 cattaaagat ctcgccgttc agtaccgcga tatcccgctg ctgtctcgta cccacggtca
     4741 gcccgccacg ccgtcaacca tcggtaaaga gatggcaaac gtcgcctacc gtatggagcg
     4801 ccagtaccgc cagcttaatc aggtggagat cctcggcaaa atcaacggcg cggtcggtaa
     4861 ctataacgcc cacatcgccg cttacccgga agttgactgg catcagttca gcgaagagtt
     4921 cgtcacctcg ctgggtattc agtggaaccc gtacactacc cagatcgaac cgcacgacta
     4981 cattgccgaa ctgtttgatt gcgttgcgcg cttcaacacc attctgatcg actttgaccg
     5041 tgacgtctgg ggttatatcg cccttaacca cttcaaacag aaaaccattg ctggtgagat
     5101 tggttcttcc accatgccgc ataaagttaa cccgatcgac ttcgaaaact ccgaagggaa
     5161 tctgggcctt tccaacgcgg tattgcagca tctggcaagc aaactgccgg tttcccgctg
     5221 gcagcgtgac ctgaccgact ccaccgtgct gcgtaacctc ggcgtgggta tcggttatgc
     5281 gctgattgcg tatcaatcca ccctgaaagg cgtgagcaaa ctggaagtga accgtgacca
     5341 tctgctggat gagctggatc acaactggga agtgctggct gagccaatcc agacagttat
     5401 gcgtcgctat ggcatcgaaa aaccgtacga gaagttgaaa gagcttactc gcggcaagcg
     5461 cgttgacgcc gaaggcatga agcagtttat cgacggtctg gcgttgccag aagaagagaa
     5521 agcccgcctg aaagcgatga cgccggctaa ctacattggt cgcgccatca ccatggttga
     5581 tgaactgaaa taaactcgta tcagtgccgg atagcgatgc tgtccggcct gcttattaag
     5641 attatccgct ttttattttt tcactttacc tcccctcccc cgctggttta tttaatgttt
     5701 acccccataa ccacataatc gcgttacact attttaataa ttaagacagg gagaaataaa
     5761 aatgcgcgta ctggttgttg aagacaatgc gttgttacgt caccacctta aagttcagat
     5821 tcaggatgct ggtcatcagg tcgatgacgc agaagatgcc aaagaagccg attattatct
     5881 caatgaacat ttaccggata ttgcgattgt cgatctggga ttgccagacg aggacggtct
     5941 gtcactgatt cgccgctggc gtagcaatga tgtttcactg ccgattctgg tattaaccgc
     6001 ccgtgaaagc tggcaggaca aagtcgaagt attaagtgct ggcgctgatg attacgtgac
     6061 taaaccattt catattgaag aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag
     6121 cggtctggct tcacaggtca tttcgctacc cccgtttcag gttgatctct ctcgccgtga
     6181 attatctatt aatgacgaag tgatcaaact gaccgcgttc gaatacacca ttatggaaac
     6241 gttgatacgc aataatggca aagtggtgag caaagattcg ttaatgctcc aactctatcc
     6301 ggatgcggag ctgcgggaaa gccataccat tgatgtactg atgggacgtc tgcgcaaaaa
     6361 aattcaggca caatatccgc aagaagtgat taccaccgtt cgcggccagg gctatctgtt
     6421 cgaattgcgc tgatgaaaaa attactacgt ctttttttcc cgctctcgct gcgggtacgt
     6481 tttctgttgg caacggcagc ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg
     6541 ctgatcggtt atagcgtcag tttcgataaa actacgtttc ggctgttacg tggcgagagc
     6601 aatctgttct atacccttgc gaagtgggaa aacaataagt tgcatgtcga gttacccgaa
     6661 aatatcgaca agcaaagccc caccatgacg ctaatttatg atgagaacgg gcagctttta
     6721 tgggcgcaac gtgacgtgcc ctggctgatg aagatgatcc agcctgactg gctgaaatcg
     6781 aatggttttc atgaaattga agcggatgtt aacgatacca gcctcttgct gagtggagat
     6841 catacgatac agcaacagtt gcaggaagtg cgggaagatg atgacgacgc ggagatgacc
     6901 cactcggtgg cggtaaacgt ctacccggca acatcgcgga tgccaaagtt aaccattgtg
     6961 gtggtggata ccattccggt ggagctaaaa agttcctata tggtctggag ctggtttatc
     7021 tatgtgctct cagccaatct gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg
     7081 agtttacgcc ccatcgaagc cctggcaaaa gaagtccgcg aactggaaga acataaccgc
     7141 gaattgctca atccagccac aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga
     7201 ttgttaaaaa gtgaacgcga acgttacgac aaatatcgta caacgctcac cgacctgacc
     7261 catagtctga aaacgccact ggcggtgctg caaagtacgc tgcgttctct gcgtagtgag
     7321 aagatgagcg tcagtgatgc tgaaccggta atgctggagc aaatcagccg catttcacag
     7381 caaattggct actacctgca tcgtgccagt atgcgcggcg ggacattgct cagccgcgag
     7441 ctgcatccgg tcgccccact gctggacaat ctcacctcgg cgctgaacaa agtgtatcaa
     7501 cgcaaagggg tcaatatctc tctcgatatt tcgccagaga tcagctttgt cggtgagcag
     7561 aacgattttg tcgaggtgat gggcaacgtg ctggataatg cctgtaaata ttgcctcgag
     7621 tttgtcgaaa tatctgcaag gcaaacagac gagcatctct atattgtggt cgaggatgat
     7681 ggccccggta ttccattaag caagcgagag gtcattttcg accgtggtca acgggttgat
     7741 actttacgcc ctgggcaagg tgtagggctg gcggtagccc gcgaaatcac cgagcaatat
     7801 gagggtaaaa tcgtcgccgg agagagcatg cttggcggtg cgcggatgga ggtgattttt
     7861 ggtcgccagc attctacgcc gaaagatgaa taaatatgtc catacttcac gcattacgtt
     7921 aagcatccgt tataatcggt tgcagatacc agcctgtgga tgcttaacat ggaataccaa
     7981 ctcactctta actggcccga ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta
     8041 aaacgcggct ttaataattt tattgacccg atctctccag acgagttggc gggtctggcg
     8101 atggaaagcg aagtcgacag tcgactggtc agtcaccagg atggcaaatg gcaggtcagc
     8161 cacggtccgt tcgaaagcta cgatcatctc ggtgaaacta actggtcatt gttagtgcaa
     8221 gcagtgaatc actggcatga gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg
     8281 gactggcgta ttgatgatct gatgatctct ttttctgtac ctggcggcgg cgtcggcccg
     8341 catctcgatc agtacgacgt gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg
     8401 ggcgaaaagc tgcaaatgaa acagcactgc ccacatccgg atctgttaca ggtcgatccg
     8461 ttcgaagcca tcattgatga agaactggag ccaggcgata ttctttatat tccgccagga
     8521 ttcccccatg aaggctacgc gctggaaaat gcgatgaact attccgtcgg ttttcgcgca
     8581 ccaaatacgc gggaactgat tagcggattt gccgattatg tgctgcaacg tgaactgggc
     8641 ggcaactact acagcgatcc tgatgtttca cctcgcgctc atcctgcgga tgttctgccg
     8701 caagagatgg ataaactgcg tgagatgatg ctcgaattga tcaaccagcc ggaacacttt
     8761 aagcaatggt ttggcgagtt tatatcccag tcacgccatg aactggatat cgcgccgcca
     8821 gagccgcctt atcagccgga tgaaatctac gatgcgctga aacaaggtga agtgctggtg
     8881 cgtctgggtg gtctgcgcgt attgcgcatt ggcgacgacg tgtataccaa tggtgagaag
     8941 atcgattccc cgcaccgtcc ggcactggat gcactcgcca gcaacattgc gctgactgcg
     9001 gagaattttg gcgatgcgct ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc
     9061 aatagcgggt attggttctt cgaagggtaa gtttggattt aagccggatg cggcatcaca
     9121 tgccgcatcc cttttcgctt acttccgttg cgccgttaac tcggcaatac ggacgatcac
     9181 ctgcaccgct ttttccatac cttccagagt cacaaactca tgcttaccat gatagttgta
     9241 accgccagtg aacaggttcg ggcacggtaa tcccataaac gacaactgcg cgccgtcggt
     9301 accgccgcgg atcggtttca gttccggttc aatatcgcag tcacgcatcg cctgctgggc
     9361 gatatcgaga atatgcggat gctcaaccac tttctcgcgc atattgtagt aactgtcttc
     9421 aatcaccaat tcaatgtagc aatcaggatg taaccctttg cccacttttt tggcgatctc
     9481 catcatttta cgtttacgcg cttcaaactg tttacggtcg aaatcacgga tgatgtagtg
     9541 catatcggcc cgttcaacgg tgcctttcat gctcgccagg tgatagaaac cttcatagcc
     9601 ttctgtcatt tccgggcttt catccgccgg aacttccgca tgaatacgtg ccgccagcga
     9661 cagcgcattc accattacac ctttcgctgt gcctgggtga acgttattgc ctacaatttt
     9721 gatattgacc gacgcagcgt tgaagttttc aaactccagt tcgcctacgc caccaccatc
     9781 aacggtgtaa gcccagcggg catcgaaggc gtcaacatca aaatgtttcg cccctttacc
     9841 cacttcttca tccggggtaa aggcgacgcg aatatcacca tgcggaatgt ttttttgttg
     9901 taataccgcc agcgcggtca tgatttctgc aatacctgct ttgtcatcgg cacctaacaa
     9961 ggttttacca tcggtggtaa tcagcgtctg acccagtagc tgatgcagca ccgggaacat
    10021 aaccggtgat aaaacttcat cgccgatacc cagcgcaata tcgccaccgc gatagttttc
    10081 aacaatttgc ggattcacat ttttgccgct gcaatccggt gaggtatcca catgagaaat
    10141 aaagccaatc gccgggatat cgccagggac gttagccggt aacgtcgcca tcaaagtgcc
    10201 cttctcactt aaggtcacat tgataagccc catctcttcg agctgctctt tcagcagatg
    10261 caataacttc cattggcctt ccgtgctggg aacctgtctc acccctgcct ttgattgggt
    10321 atccagagac acgtagttca aaaatcgctc aagtagttta tccatgtagt caccctcact
    10381 ttttgtgaca acattattaa gaagcaagaa aagacaaata ttgcgtcagg tcacttttac
    10441 ccctgcaagc gggaatattt atcagcatta ccttaatgaa tataaagcta agccagtaat
    10501 tcacaacaaa gattggcgat tcaagcggtt tgccgtagaa ttaccgccct cattaagagt
    10561 caccaaggtg gttaaccaca aaccccgcat cggtaagcca tccgttgcgt ttacatggga
    10621 cagagtaaaa aattgaataa acaaccgagt tcgctttcac cgctggtgca attggcggga
    10681 attcgcaaat gctttgatgg taaagaggtc attccccagc tggatctgac tatcaacaat
    10741 ggcgagttcc tcacgctgct tggcccttct ggctgcggta aaacaaccgt tcttcgcctg
    10801 attgcaggtc tggaaactgt tgattccgga cgcatcatgc tggataacga ggacatcacc
    10861 cacgttccgg cggaaaaccg ctatgtgaac actgttttcc aaagctacgc acttttcccc
    10921 cacatgaccg tgttcgaaaa tgtggccttt gggttacgca tgcaaaaaac ccccgctgct
    10981 gaaattacgc cccgcgtgat ggaagccctg cggatggtgc agctggaaac cttcgctcaa
    11041 cgcaaaccgc atcagctctc tggtggtcaa cagcaacgcg tcgccattgc ccgcgcggtg
    11101 gttaacaagc ctcgtctgtt gttgctggat gagtcgctct cagcgctgga ttacaaactg
    11161 cgcaagcaaa tgcagaacga gctgaaagcg ttacagcgta agcttggcat tacattcgtc
    11221 ttcgtgactc acgaccagga agaagcactc accatgtcgg acaggattgt ggtgatgcgc
    11281 gatggtcgca ttgagcaaga cggcacgccg cgtgaaatct atgaagagcc gaaaaacctg
    11341 tttgttgctg ggtttattgg cgaaatcaat atgtttaacg ccactgtcat cgaacgtctc
    11401 gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat gtaatatcta cgttaacttc
    11461 gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa
    11521 gagattaacg acgacaacca cgctgaaggg ctgattggtt acgttcgcga gcgtaactac
    11581 aaaggcatga cgctggagtc ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc
    11641 gaattcttca atgaagacga tcctgacttt gaccactctc tcgaccaaaa aatggccatt
    11701 aattgggtag aaagctggga ggtcgtactg gctgatgaag aacacaagta agttccagaa
    11761 tgtagtgatt gtcactattg tcggttggct tgtgttgttt gtctttctgc ccaacctgat
    11821 gatcattggc accagctttt tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac
    11881 gctggataac tacacgcgtc tgctcgatcc gctctatttt gaagtgctat tgcactcgct
    11941 gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg ggctatcctt ttgcctggtt
    12001 tctggcgaag ctgccacaca aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt
    12061 ctggaccaac tcattgattc gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta
    12121 tctcaacgag tttttgctct ggctgggcgt tatcgataca ccaattcgca tcatgttcac
    12181 acccagtgcg gtgattatcg gtctggttta cattctgctg ccgtttatgg tgatgccgct
    12241 gtactcgagt atcgaaaaac tggataagcc gttgctggag gcggcgcgcg atctcggtgc
    12301 cagcaagttg cagaccttta tccgtatcat tatcccactg acgatgccgg ggattattgc
    12361 cggatgcctg ttggtgatgc tgccagcgat gggcctgttc tatgtgtccg acctgatggg
    12421 cggtgcgaaa aacctgctga tcggtaacgt catcaaagta cagttcctca atattcgtga
    12481 ctggccgttt ggtgcagcca ccagcattac gctgactatc gtaatgggcc tgatgttgct
    12541 ggtttactgg cgcgcttctc gtttgctgaa taagaaggtg gaactcgaat gatcggtcga
    12601 ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc tgtatatccc aatcattatt
    12661 ttgattgtga actcctttaa cagctcgcgc tttggcatca actggcaggg ttttaccact
    12721 aaatggtata gcctgctgat gaacaacgac agtctgttgc aggcagctca gcattcattg
    12781 acgatggcgg tgttttcagc gacgtttgct acgcttatcg gttcactgac tgcagttgcg
    12841 ctgtaccgtt atcgctttcg cggtaagcca ttcgttagcg gaatgctgtt tgtggtgatg
    12901 atgtcgccag atattgtgat ggcgatttct ctgctggtgc tgtttatgct gctgggtatt
    12961 cagcttggct tctggtcgct gctgttctcg catatcacct tctgcctgcc atttgtggtg
    13021 gtgacagtgt attcgcgcct gaaaggtttt gacgtgcgaa tgctggaagc ggcgaaagat
    13081 ctcggtgcca gcgaatttac cattctgcga aaaatcattc tgccactggc aatgccagcg
    13141 gtagcggcgg gctgggtgtt aagctttacc ctgtcgatgg acgatgtagt agtttcttcg
    13201 ttcgtcaccg gaccaagcta tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc
    13261 ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc gctggtgatg
    13321 gtaattgcca gccagcttat tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa
    13381 tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg
    13441 ctcacgccga tgacaacaac acgctgtatt tttacaactg gaccgagtac gtgccaccag
    13501 gactgcttga acagttcacc aaagaaaccg gtattaaggt tatctattcg acttacgagt
    13561 cgaacgaaac catgtatgcc aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg
    13621 ttccttcaac ctattacgtc gataaaatgc gtaaacaagg gatgatccag aagatcgaca
    13681 agtcgaagtt aagcaatttc agcaacctcg atccagacat gctcaataag ccgtttgacc
    13741 cgaataacga ctactccatt ccgtatatct ggggtgcgac ggcgattggt gttaatggtg
    13801 atgcggtgga tcccaaatcc gttaccagct gggccgatct gtggaagcct gagtacaaag
    13861 gcagcctgct gctgaccgac gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg
    13921 gctactccgg taacaccacc gatccgaaag agattgaagc tgcatacaac gagctgaaaa
    13981 aactgatgcc aaacgtcgcg gcgtttaact ccgataaccc ggcgaacccg tatatggaag
    14041 gcgaagttaa cctcggcatg atctggaacg gttctgcttt cgttgcacgc caggcgggta
    14101 cgccaattga tgtggtgtgg ccgaaagaag gcggcatttt ctggatggac agcctggcga
    14161 tcccagcaaa tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc
    14221 cggatgtggc aaaacaggtt gctgaaacta tcggttatcc aacgccaaac cttgcggcgc
    14281 gtaagctgtt aagtccggaa gtggcgaacg ataaaacgct ctacccggat gctgaaacca
    14341 ttaaaaatgg tgaatggcag aatgacgttg gcgcagccag cagcatttat gaagagtatt
    14401 atcagaagct gaaagcagga cgttaattca gcaagcgggc ggtggtatta ccgcccgttt
    14461 tttatttagc aaggatgtgg ctaatgagtc aggacagtaa agttttcttt cgtatattcc
    14521 tcggtatcgg tctggtactg atcctcattt ctgttgttat cttttataac cagttcacct
    14581 atagcaaaga cgcgattcat actgaaggcg tcattgttga taccgtctgg cactccagtc
    14641 attctcatcg cacgggtaaa aatggttcgt ggtatcccgt cgttgccttt cgaccgacgc
    14701 ctgattacac gctaattttc aattcgagta tcggcagcga tttttatgaa gacagcgaag
    14761 gcgataaagt taatgtttat tattctccag ggcatcctga aaaggctgag ataaataacc
    14821 cgtgggttaa tttttttaaa tgggggttta tcggcattat gggcgttatt tttatcgccg
    14881 tcggtttatt aatcagtatg ccctcctcta aaaagtcacg caggaaacgg aagtcccgtc
    14941 catgaaaaaa atcataatac tgctcagttt actgatactg ctgcctctga cagcaaccag
    15001 taagccctta atcccaataa tgaagacact ttttacggat gttacaggta ctgtaccgga
    15061 tgctgaggag attgcgcgga aagcggagct ttttcgtcaa caaaccggga ttgcgccgtt
    15121 tatcgtggtt gtacctgacg ttaatgaagc aagcttcagg caaaatggca aagcgatgct
    15181 ggcgcatgcg tcatcttcat tgagtgatgt aaaaggcagc gtcctgttac tttttaccac
    15241 ccgcgagcca cggttaatta tgataaccaa cgaccaggtt gaaagcggtc tggacgataa
    15301 acatctcggc cttctggtag aaaatcacac tcatgcttat ttacatgcgg acctctggta
    15361 tcagggaatc aataatgcat tggctgtttt acaagcacag atattaaaac aaccgacgcc
    15421 gccactgaca tattatccgc atcccggaca acagcaggag aatgatcctc ccggcagtac
    15481 cacgatatta ggtctttttg cctgggcagt tgcattcatc gtcttgacta cattttttaa
    15541 ctacacaacg cgccttaatt atgcattaaa gtttgcagtg gcgatgactg ttgccaatat
    15601 gggttatcag gctttatgtt tgtacatcga taatagcttt gcgattacca gaatatcgcc
    15661 gctctgggca gggctgattg gtgtctgtac gtttattgcc gcgttgttat tgacgagtaa
    15721 acggtaaaag aggcagtaaa gtgtgcgttt attatgccgg atgcggcgcg aacgccttat
    15781 ctggcccaca aaacccgcaa attcaattaa ttgcgtcccc ttgtaggcct gataagcgta
    15841 gcacatcagg caatgcttcc tgctttataa gcccttcagc aacttttcaa caaactctgg
    15901 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctggcttgg
    15961 ttcaagattc agctccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    16021 tgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcggcca tcgacaacgc
    16081 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    16141 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    16201 gagaacctga ccactttgcg aacagcgaac tttcagcagt tccccatgca tatgaatgac
    16261 attggtatta cctgcgcgtt catgcaggtt gtcgatattc tgcgtcacca gcaaaaagcg
    16321 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    16381 tggctgctgt agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    16441 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    16501 ggcgcgaaag gtacgaatac ctgattccgc agaaatacct gcccctgtca gtacgagtac
    16561 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaacac gctgacgcaa
    16621 acgctcgcgc agacggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    16681 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    16741 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    16801 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    16861 aatgacgacc aggtcagggt caacaatggt caggatattt cccagacaaa ccgctaataa
    16921 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    16981 aatttcggga gcctgcaacg gttgatgata atagtgttga tacagccacg caaaaccgcg
    17041 accagacaga taattttcaa tgcagccatg ctgaccacag ccgcagcggc gtaacgggaa
    17101 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    17161 aatatagctt ttaccggtaa ttggtttacc gttgaaaatc agcccgccgc caacgccggt
    17221 gccgagaatc aaccccatca ccagtggata ttgagtaaat tcatcatccc aggcttctga
    17281 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    17341 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    17401 ttctgtttcc ggcattcccg gaataccgat gccgacagag cctttacagc caaaacgttg
    17461 gtcagcttca gctaccagct cacacactgc atctaaaaat gcgtcatagc tgtcacgcgg
    17521 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    17581 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    17641 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgtcgc gccggatacc
    17701 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    17761 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    17821 tcaacttttc aatccactca ataatcgggg taagttgcag tgaaacaact acgccgataa
    17881 tcacaccaca caggctaccg aatagccctg ccagcaatcc ataccagaca aagatggcgc
    17941 gaattaaacc atctttcgcc cccagcgttc gtaatactgc gatatcgcca ctcttgtctt
    18001 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    18061 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    18121 caatccagct tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    18181 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    18241 caagatattg ctgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    18301 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    18361 cgggattcga gttggggatc ataatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    18421 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    18481 ccccctgaac aaacgagggc aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    18541 tcacctggat tgcgcgcaga ttcgcgccgc tctccaccag cccggtgaaa ttgatatacg
    18601 gcgcagcggc ggcaatgccc ggcactttct gcacgttatc cagggcttcc tgccagttag
    18661 tccacggctg atctaccgcc tcaatttcac catgcggcac caccgccaga atacggttgt
    18721 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    18781 gggcaatgcc aatggtagaa atgactgaga tcagcgacac catgccgccg cgtcgccgtc
    18841 cgcggctaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    18901 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    18961 cgccagttgc aggtcgtgag tgaccaccaa gaaggcggtg ccctgcaagc gattcaactc
    19021 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatca aggttaccgg taggttcatc
    19081 cgccagtacc agacgagggt tattgaccag cgcacgggca atcgccactc gctggcgttc
    19141 gccaccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    19201 catttccaac gctcgactgt tgatttcagc gggctttttc ttgccaatca gcagcggcat
    19261 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    19321 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggctgacc
    19381 gttaaagatc acatcgccgg aggttggcgt atccagcccg cccagcaggt gcagcaaggt
    19441 gcttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    19501 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    19561 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttgt gtggcggcag
    19621 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    19681 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    19741 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccaagga
    19801 tcgcaccgat aataccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    19861 gagttaagcc ttgcgtttgc aggatcgcta cttcgccctg cttctccatc accatcagcc
    19921 ctagcgaggt aataatgtta aacgccgcta cggcgacaat caggctcagc agcaagccca
    19981 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    20041 gccatttgct gccttcaggc agtttttgtt gacttaatga gtcgactttc agcggctcat
    20101 ccagccacaa tcgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    20161 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcagcg aaagtgccaa
    20221 tcacattgaa cagacgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    20281 gcaccatcac gcggatttga tcaccgcgat tgacgcccaa ctgtgaggca agctgttcac
    20341 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    20401 gcgttagtgg atctttttgc gccgggtcga tacccagcat caccccgacc gccacgctgc
    20461 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    20521 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    20581 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    20641 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    20701 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    20761 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    20821 gcgaaagcgg gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    20881 tagcagcaaa tcaatcgact aaagggcctc attttctcat tggccttgtg gcctgatctt
    20941 tatctgaaaa ataaccaaat acagacagat acgcattaca ccacgcgact ggcccctcag
    21001 gatcaacagc atgttaaata aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    21061 attaaccaga tcaaagggtt atgtatttgt ctggtagtga tttatcactc ggtgattacc
    21121 ttttatccgc atctgagcac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    21181 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatatccggc
    21241 tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga taaacgcatc
    21301 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    21361 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcgtatgcc
    21421 gattccaccg gtgagttcct gcgcgggatg atcaccgcca gcaccagctt gtggtatctg
    21481 tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc gctgccacta
    21541 ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc gtggtggggc
    21601 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    21661 ttaatgacct acgttaaaga ggtgccgttg cgccgccatc tgctgatggc ttctttgctg
    21721 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    21781 attgtggtga tcatgaagct gttttatcaa tacgagcaac gtttcggtat gcgctccacc
    21841 agcctgctga atgtgattgg caccaacacc attgctatct acaccaccca tcgcattctg
    21901 gtagagatat tcagcttaac tctgcttgcg ccaatgaacg cggcacgctg gtcgccgcaa
    21961 gtcgaactga cactcctgat ggtttacccc tttgtcagtt tgttcatctg tactgttgcg
    22021 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    22081 tcactgcccg ctgccgtcag ttactcccgc taagccatcg ccccagccgg ggcgattcaa
    22141 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    22201 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    22261 tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgctgcctg
    22321 cgcgacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac tcattgcacc
    22381 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagtttaccg atcaaatggt
    22441 gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc atcaggacat
    22501 tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg gcgtactgat
    22561 tgttccggtg aatacgctta tgcagcgcgt ttgcccgcac agttttctcc acggtcatgc
    22621 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    22681 cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca cgcgcggcgc
    22741 gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt tctttgatga
    22801 tgaaatcgac agcctgcggg tgtttgacgt cgatagccag cgcacgctgg aggaagtaga
    22861 agagatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    22921 ccgcagccag tggcgtgata ccttcgaagt gaagcgcgac ccggaacata tttatcagca
    22981 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt tcttcagcga
    23041 accactgccg ccgctgttca gttatttccc tgccaatacc ttgctggtga atactggcga
    23101 tctggaaaac agtgccgaac gtttccaggc tgacacgctg gcgcgttttg agaatcgcgg
    23161 cgtcgatccg atgcgtccac tgttgccacc acaatcgctc tggctgcggg tagacgagct
    23221 cttttcagag ctgaaaaact ggccgcgtgt gcagctaaaa actgaacatt taccgacaaa
    23281 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    23341 aaaagcgccg ctggatgcgc tacgtaagtt cctcgagact ttcgacggtc cggtggtgtt
    23401 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac gaattaaaat
    23461 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atctgatgat
    23521 tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaac ctggcgctga tttgcgaaag
    23581 cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca ccatcaaccc
    23641 cgatacactg atccgcaacc tcgcggagct gcatattggc cagccggtgg tccatctgga
    23701 gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gcgggcggca ttactggcga
    23761 gtatttgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    23821 tctgattagc cgttacgccg gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    23881 cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcggcaga
    23941 attgctggat atctacgcgc aacgcgccgc caaagagggc tacgcgttta aacacgatcg
    24001 tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcaca
    24061 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    24121 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    24181 taaccacaag caggtggcgg tgctggtgcc taccaccctt ctcgcgcagc agcattacga
    24241 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    24301 cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa tcgatattct
    24361 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    24421 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    24481 gaacgtggat attctgacgc ttaccgctac gccgatccca cgtacgctga atatggcgat
    24541 gagcggaatg cgcgatctgt cgattattgc cacgccgccc gcccgtcgtc tggcggtcaa
    24601 aacgtttgtc cgcgagtatg acagtctggt ggtcagggag gcgatcctgc gtgaaatttt
    24661 gcgcgggggg caggcttatt atctttacaa tgatgtggaa aacatccaga aagccgccga
    24721 acggctggcg gaactggtgc cagaagcgcg gattgccatc ggtcacgggc agatgcgtga
    24781 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    24841 taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta tcattgaacg
    24901 cgcggatcac ttcggtctgg cgcagctgca ccagttgcgc ggtcgcgtcg gacgttcgca
    24961 tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta ccgatgcgca
    25021 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    25081 gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa gtggctcaat
    25141 ggaaactatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg atgcgctgaa
    25201 agccggacgc gagccgtcgc tggaagatct caccagtcag caaactgaag tcgagctgcg
    25261 gatgccgtcg ctgttgccgg atgatttcat tcctgacgtg aatacgcgtt tgtcgttcta
    25321 taaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag ttgagcttat
    25381 cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg ctggatattg cccgtctgcg
    25441 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggcgtgat
    25501 cgaatttgcc gagaagaatc acgttaatcc ggcatggctg attggtttgc tgcaaaaaca
    25561 gccgcagcat taccgccttg atggtccgac gcgcctgaag tttattcagg atttgagtga
    25621 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgccat
    25681 cgcttgatgg ttagattggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    25741 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    25801 acaataacct tatatttatt atggattttt ggtgatgatc aaaacgcgtt tttctcgctg
    25861 gctaacgttt tttacgttcg ccgctgccgt ggcgctggcg ctaccggcaa aagccaacac
    25921 ctggccgctg ccgccagcgg gcagtcgtct ggttggcgaa aacaaatttc atgtggtgga
    25981 aaatgacggt ggttctctgg aagccatcgc caaaaaatat aacgtcggct ttctcgctct
    26041 gttacaggct aaccccggcg ttgatcctta cgtaccgcgc gcgggcagcg tgttaacgat
    26101 cccgttgcaa accctacttc cagatgcgcc gcgcgaaggc attgtgatca atattgcgga
    26161 gctgcgtctc tattactacc cgccgggtaa aaattcggta accgtgtatc caataggtat
    26221 tggtcagttg ggtggtgaca cgctgacacc gacaatggtg accaccgttt cagacaaacg
    26281 agcaaaccca acctggacgc caacggcaaa catccgcgcc cgttataaag cacagggaat
    26341 tgagttgcct gcggtagtgc cggctggacc ggataatcca atgggccatc atgcgattcg
    26401 tctggcggcc tatggcggcg tttatttgct tcatggtacg aacgccgatt tcggcattgg
    26461 catgcgggta agttctggct gtattcgtct gcgtgatgac gatatcaaaa cactctttag
    26521 ccaggtcacc ccaggcacca aagtgaatat catcaacact ccgataaaag tctctgccga
    26581 accaaacggt gcgcgtctgg ttgaagtaca tcagccgctg tcagagaaga ttgatgacga
    26641 tccgcagctg ctgccaatta cgctgaatag cgcaatgcaa tcatttaaag atgcagcaca
    26701 aactgacgct gaagtgatgc aacatgtgat ggatgtccgt tccgggatgc cggtggatgt
    26761 ccgccgtcat caagtgagcc cacaaactct gtaaatcctg gataaaaaaa atccccgcga
    26821 cacggcatgt cgcggggaaa aaatcagcag tagcaggcat taatgagggt taatgcttat
    26881 ttataaatta ctgctgttcc atggagggta tttggaccgg ttacagaagt aatacggaat
    26941 gattttgcgc ccatctcatc cgctttttgc gccagctgct cttccagcga tcccagattt
    27001 gtccccgcgt tagcactgat agtaccgact ttttgttggc cttctggcgt tgactgaact
    27061 tcgacagcag caaagctggc aaatgacatg gagcttaaaa tcgccgcagc gatgagggtt
    27121 tttacgtttt tcataatagt ggccttatgc agatgaatga cgtcgcaagc aattacttaa
    27181 cgatcgataa ctaaatgata gatgtgatct ggatcacata caagatattt tttataacaa
    27241 tcattaatta atttaaaaat ctttaaattt cagttaaata gaaagcaatt atttttatgg
    27301 gatctggact ggtgaacgga ggtgattatt tttataataa cctttcattc acagtcacca
    27361 ggtacaacgg catcatggca actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc
    27421 caaaagtgtt cgacagggat gcagcgcttg ataaggccat gaaattgttc tggcaacacg
    27481 gttatgaagc gacttctctt gcggacctcg tcgaagcgac cggagccaaa gcgcccacgc
    27541 tatacgcgga atttaccaac aaagaggggt tatttcgcgc cgttctcgac cgctatatcg
    27601 atcgttttgc cgctaagcat gaagcacagc tgttttgtga agagaaaagc gtggagtctg
    27661 cgctggctga ctattttgct gccatcgcca actgctttac cagcaaagac accccggcag
    27721 gctgcttcat gatcaacaac tgcaccaccc tctccccaga ttccggagat atcggcaata
    27781 cgctgaaatc acgccatgcg atgcaagagc gcactttgca gcagttttta tgtcaacgac
    27841 aagcgcgcgg tgaaatcccg acccactgtg acgtgacgca tctggctgaa ttccttaatt
    27901 gtattattca ggggatgtcg atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc
    27961 agattgtccg aacgactttg cgtttatggc ccgaactggt gaaataaggc gcaaaaaaag
    28021 cccctcagcc tgagcgaatg aggggctaaa ttgcagagta cagccttgtt atactttgtt
    28081 attcaaaatc agttgcccgt tgctatctag cgggatctgc gtacccggat cgcggtccat
    28141 gcggattttg ccctgctgat cgccaatctt ataggtcaca tcataaccga gcattttttc
    28201 tgacttgtca tacaccgttt tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg
    28261 gagagagccc tggatctggt taccggcata gccacctccc agcgccccca caacagtggc
    28321 gacatcttta ccgcgaccac cgccaaactg atgccctatc acgccgccag caacagcgcc
    28381 gagcaccgac ccggtaatgc gattttcatc ctgcaccggt tgacgatggg tcactgtgac
    28441 gttgcgacac tcctgacgcg gtgttttaac cgtttccttg attggggttg cagaaacaac
    28501 ctgagcgtat tgcgggcccc gttcaaacac gttcagactg gccactgccg ctacgcccag
    28561 cgcagctgcg acaccaatcc cgatacccgc caacattgat ttattcacgg gaacacctcc
    28621 ttctttgcag ttatgccaat catgcaatcg aaggagaaaa gcagctatca gacagaagat
    28681 gaaaagcgcg ggaatggctg gaaatactta aggattcgga ggagtctgaa ggaatgcctg
    28741 atgcgacgct taacgcgtct tatcaggcct accgctcgta ccatttgtcc agggcggata
    28801 aggcgtttac gccgcatccg ccagtacaag ccgattaatg caacttcaaa cgtggacgga
    28861 taacgcggtt aatactcccc accagcatca ttaatccggt tttaaagtaa ccatgcagcg
    28921 caatctgatg cattcggtat agcgagatgt atacaaagcg cgcaattcgt ccttcaatca
    28981 tcattgagcc gcgcgtcagg ttacccatca ggctaccgac ggtggagaag ttcgacagcg
    29041 ataccagaga accgtgatct ttatactgat aatttttcag cggcttacca ttcatctgcg
    29101 ccagaatgtt gttcattgcg caagttgcca tctggtgtgc agcctgagcg cgcggcggaa
    29161 caaagccccc ttccggacgc gggcatgacg cgcaatcgcc aatagcgtaa atgtctggat
    29221 cgcgagtggt ttgcagcgtc ggttccacca ccagctggtt gatacggttg gtttcaagac
    29281 caccgatatc tttcaggaag tctggcgctt tgatcccggc tgcccacacc atcagatcag
    29341 cctcaatata ttcgccatct ttagtatgca ggccgccttc atcagcactg gtgaccatgg
    29401 tttgcgtcag cacgcgaacg ccaagtttcg ttaactcatt atgggccgca gcagagatac
    29461 gtggcggtaa tgcaggcaaa atacgttcgc ccgcttctac cagcgttacg ttcagggctt
    29521 cgttggtcag acctttatag ccgtagctgt gcagttgctt gaccgcgttg tgcaattcag
    29581 cggagagttc tacacccgtc gcgccgccac cgacaatcgc aatgttcact ttgccattcg
    29641 cgcccaggtt ggcggagtat ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg
    29701 cctggtgcgg gttatcgagg aaaatgcagt tctctttgac acctggcgta ttgaaatcgt
    29761 tagaggtgct acccagcgcc attaccaggg tgtcataggc gattttacgt tccggaacca
    29821 gcagttcacc tttctcatcg cgcagttctg caatagtgat tgttttcgct tcacgatcaa
    29881 tatcaatgac ggaacccagc tggaactgga aaccatgatt gcgcgcatgg gccagatagc
    29941 tcaacgcatc gacgccttca tcaagcgagc cagtcgccac ttcgtgcagc agcggtttcc
    30001 acaggtggct gtggttacga tcgaccagcg taattttggc ttttttcttg cgtcccagct
    30061 tatgccccag ctgtgttgcc atttccagcc caccagcacc gccgccgaca atcacaattt
    30121 ttttcaatgg cgtagtcaac gtgaccccct taaatttatt aaccaattgt taattaatag
    30181 ttattaatat agctcttaat taacaacagg ttacagcaat gaaaatgttc ttcgctattg
    30241 agaataacat gaatggtgca ttggtcatac caaaattgat gttaatcaag ttttgttgct
    30301 gaaaagatag gcgactttac cgggtgagat caaaaaacag agtttgttgt ttattatttg
    30361 agcgaatttg tgagagtgat atagagtgag gtgtgtatgg atggcgttcg gtaggcctga
    30421 taaaaggcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtgaacg ccttatccga
    30481 cccacagaat ccggagcgca tcaggtaccg gcaaggttac ctgatgcgac gagggcattt
    30541 acccgagggt tttgaacgct ttaatgcgct gcaaatgcgg ggagatattc ttgaatttgt
    30601 gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca gatgtccgtt
    30661 ggctgttaag cgcttcatca ttacgcgaca aaatcaccag gcaacgatcg cgattctttt
    30721 cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg cgatcaatct
    30781 tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg cggatatcgc
    30841 aaagaaaacc aatccgttcc gcccagtatc cgcccagacc aacgccgcaa attagcggac
    30901 gctcgtcaac gttcagttgc aacattttgt ccacttcttt gagcagatgc tgcatatcat
    30961 gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata aactgcaatt
    31021 gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa tagataatca
    31081 tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca gcgttcatgt
    31141 aactgattca gacgggcgct gatcgctttc cagcgagccg agtccatcag ttcctgtcgc
    31201 gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg tgacagatta
    31261 tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc gcaacccgcg
    31321 tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggctcc ttccatcgat
    31381 aaatcgtcag agaaaatcac gccatcaaaa cccagttcct gacgcaaaac ggttttcagc
    31441 caatagggag aaccgctcgc cggacgcggg tcgacatcac tgtagatcac atgcgcaggc
    31501 ataatggcgt cgagtttatt ttcgcggatt aaggaactga agaccgacat atctttagcg
    31561 cgaatctccg cttgcggacg tgggtcacac ggtgtttctt tgtgtgagtc tgccgttact
    31621 gcaccgtgtc ctgggaagtg tttcccggta gttttcattc cggcttcatg cataccatca
    31681 ataaaccggc tggcaattgc cagggctttt tcgggatcgg catgataaga acgctcgcca
    31741 atcgccgcgc tgatatgccc gacgtccagc actggcgcaa agctgatatc aatatccata
    31801 gcaatcattt cgctggccat caaccaacct gcctcttgcg ccagttttcc gccttcttcc
    31861 attcccaaca gcgcagcaaa tgattgtgcc gctggcaagc gggtaaaacc ttcacgaaaa
    31921 cgctgcacgc gtccaccttc ctgatcaacc gccaccacca gatgattgcg cgaagctgcg
    31981 cggatctggc gcaccagttc acgtaactgg gcaggatcat gatagttacg cgtaaagaga
    32041 atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc cagttcgtaa
    32101 ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta ttaatagctg
    32161 ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccggtttgtc gccagcggta
    32221 ctcaaaccac cctgctttga gcatcagcag ccagggaaac catcgcctga cctgacgcca
    32281 taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg
    32341 gtcagtattt tccacccaca ccgccgccag ttccagcgcg atatcaccat ctccggcata
    32401 ctcccagtcg atgagtttta accctgacgc gctatgcact aaatttccgg cgtggacatc
    32461 catatgcaat ggacttaagc gtaaaggccg tggttcccgc gctttgcgca gacgttttaa
    32521 ccttcgcagc caacccactg tccgccgcgc cggatcgctt tgctgccagt acagttccag
    32581 taacggcaac agcgttattc gccagccaaa acgcggttgt tgatgtagat aatacagcaa
    32641 gcctgccagt tcgttggtat ccggcaaata cgtttttacc tcgccgggca gatagtcgac
    32701 caccatccag tcacggagat ataaatgcgg cttcggtgca atgcatgcgg gtagttgtga
    32761 taaagcccga tactggcgca agaacgcgaa ccgcggcgca tcaggatcgt gcggctgacg
    32821 cacaacaaaa cgctggccct gatgttcaat gagaaaactc ccgccactaa gcccactatt
    32881 aaacgtcgtg acgggatgaa attgcgggaa aaagcgcgac agcaattcgt cgcgcgtgat
    32941 gggattattg ctgcgaaacg gcacctttac ctgaccagat aatttcaccc gtctgcacca
    33001 gcatcagctg catttgtagg gtcggagcgt taacgttgcc agaggcgctg gagtacagca
    33061 cgtaatgagc gccgacattg cgggcaatac ctatggcttt actacgggtg cctaaactgt
    33121 cctgcggcga caaacctaac tgttgctttg ccatcgacag ctgctgggcg gaaaccaggg
    33181 taaatttacc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct gcggcattca
    33241 gcgaaccgtt agtacggttg ttaacgctat caaccagaag gacgctaccc gcagtcaccc
    33301 cgtcagcccc aagcatctta ctgaccatcg gctgcattgc gccattccag tcataatggc
    33361 gaatatgcgg cgcaggcggt gcagtttgat cttcgtgctc aattgggcct ggctgctgcg
    33421 ggattgtcgg caccgagggc actgtgggga caggctgttg cggctcggct ggttgttccg
    33481 gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac ccggcgagaa
    33541 acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag attcacccct
    33601 tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct gccgtacagc
    33661 gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg atgcatctcc
    33721 agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc ttgcctttcg
    33781 ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac gggcttttct
    33841 gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc atcattcacc
    33901 ggaatttccg gatgtgaacg acagcccacc agcagtaaca acgccagaga caccagccca
    33961 tagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc agcggacggc
    34021 cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccgtggcgg ttggtgttca
    34081 tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt gccgctacgg
    34141 tgatcatgcg tcccagcgcc tgctcatgct cagcagacac gtcgttcaca gttggaatga
    34201 ggatatttgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg cgaaacgccg
    34261 ttaccagatc atcctggtaa acgatatcgg aggggatctc acgacgaata attttgctga
    34321 atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt gcgccatgct
    34381 acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct ttcaacctta
    34441 actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca ttattcccat
    34501 ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgtc aaatatgaat
    34561 acttatattt ctcatttgca tttacatgca aaattattcg cacactaaat ataaccgctc
    34621 atcaccctgc tccggcatga gcctgacgac aacataaacc aagagatttc agatgctttc
    34681 aacacaattt aacagggata atcaacatca aaccacctcc aaaccgtcac tgcttgccgc
    34741 ctgcatagca ctggcactct taccttccgc cgcttttgct gcaccagcca ctgaagaaac
    34801 ggttattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt acagcgtaac
    34861 gtctacctct gcgggtacca aaatgcagat gactcaacgt gatattcctc agtcggtcac
    34921 tattgttagc cagcagcgga tggaagatca gcagttacaa acgctgggcg aagtgatgga
    34981 aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt attattcccg
    35041 cggattccag attgataact atatggttga tggtatcccc acctattttg aatcgcgctg
    35101 gaatctgggc gacgcacttt ctgatatggc actgtttgaa cgcgtagaag tagtgcgtgg
    35161 cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata tggttcgaaa
    35221 acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta gctggaacaa
    35281 agaacggtat gtggcggatt tacaaagtcc actcaccgaa gatggtaaaa tccgcgcgcg
    35341 aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaaca gtgaaaagac
    35401 cttcttctcc ggcattgttg atgctgattt aggcgatctt acgacgcttt cagccggtta
    35461 cgaatatcag cgcattgatg ttaatagccc aacgtggggc ggtttaccgc gctggaatac
    35521 tgatggcagc agcaacagtt acgatcgcgc acgcagtacc gcacctgact gggcgtacaa
    35581 cgataaagag atcaacaagg tctttatgac cctgaagcag cggtttgctg atacctggca
    35641 agcgacactg aatgccaccc actccgaagt cgaatttgac agcaaaatga tgtatgtcga
    35701 tgcctatgta aacaaagcgg atggtatgct ggttgggcca tacagtaatt atggacctgg
    35761 ctttgattat gtcggcggta ccggttggaa cagcggcaaa cgtaaagttg atgcgctgga
    35821 tttgtttgct gacggtagtt atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg
    35881 cagttacagc aaacaaaaca atcgttactt cagttcatgg gccaacatct tcccggatga
    35941 aattggcagt ttctacaact ttaatggcaa tttcccacaa accgactggt caccacagag
    36001 cctggcgcag gacgatacca cacatatgaa atcgttatat gctgccactc gtgtcaccct
    36061 tgccgatccg ctgcatctga tcctcggcgc acgttatacc aactggcggg ttgatacgct
    36121 gacttacagc atggagaaaa accacaccac gccttacgct ggtctggtgt ttgacatcaa
    36181 tgacaactgg tcgacctacg ccagctatac ctctattttc cagccgcaaa atgatcgtga
    36241 cagttcaggc aaatatctgg ctccaatcac cggtaacaac tacgagctgg gtctgaaatc
    36301 ggactggatg aatagccgtc tgaccaccac gttagccatc ttccgtattg agcaggataa
    36361 tgtcgctcag tccaccggta cacctatccc cggcagcaac ggcgaaaccg cctataaagc
    36421 ggtggatggg acagtcagta aaggtgtgga atttgaactc aacggcgcaa ttaccgacaa
    36481 ctggcagctg acgtttggcg caacgcgcta tattgcagag gataacgaag gaaacgccgt
    36541 taatcctaat ctgccacgca ccacggttaa aatgttcacc agctaccggt tgcctgtcat
    36601 gccagagttg acggtcggcg gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt
    36661 gacaccgtat ggcaccttcc gcgccgagca aggtagctac gcgctggtgg atctcttcac
    36721 ccgctaccag gtgacgaaaa acttctcgtt acaggggaac gtcaataacc tgttcgacaa
    36781 aacctacgat accaacgtgg aaggttctat cgtctacggc gcaccgcgta atttcagcat
    36841 taccggcacg tatcaattct gaataacacc tgtaaaaaag gcagccatcc ggctgcctta
    36901 gtctccccaa cgtcttacgg attagtggtt acggatgtac tcatccatct cggttttcag
    36961 gttatcggat ttagtaccga aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc
    37021 tgcgcccagt ttcttcaggc cagcctgatc cactttagac acatcagcaa cgctgacgcg
    37081 cagacgggta atacatgcgt cgaggttagt aatgttttct ttaccaccaa atgcagcaac
    37141 cagagccggt gccatttcgc tggtacctgt cgcttttgca tcttcagtcg catcttcacg
    37201 acccggcgtt ttcagatcca gtgctttaat cagcacgcgg aagatggtgt agtaaacaat
    37261 cgcataaccg ataccgacga tcgggaacag ccacagtttg ctgctgttac cagacagaac
    37321 gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca cgcatcccca aaagaataca
    37381 gattgggaat gccaggcctg ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa
    37441 catgaaggag aactcgatcg gctcggtgat accggtcagg aacgaggtca gcgccgcgga
    37501 gatcataata ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc agatagcaat
    37561 tgcggcagct ggcagaccgt acattttgaa caggaagcca ccagacagtt tacccgcagt
    37621 cgggtcaccc gccatataac gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt
    37681 gtattcacca atttgcatct ggaaaggtac gttccagatg tggtgcagac caaacggtac
    37741 caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact accgggttct ggtaagcagc
    37801 ccattgagag aaggtctgga ttgcagaacc aatcggcggc caaatgaagg acagcacaac
    37861 gccagtaaag atggcagcca ggccagaaat gatcggcaca aagcgtttac cggcaaagaa
    37921 gccaagatac tcaggcagct taatacggta gaaacggtta aacatatacg ctgcaatcgc
    37981 accggagata atccctccga gtacgccagt atccgccagg tgtttagagg cgatttcttc
    38041 agcaggtaaa tgcagtacca gtggcgcaac cacggccatg gttttaacca tgatgccata
    38101 ggcaacaact gcggccagcg cggatacgcc atcgttattg gtaaagccga gggcgacacc
    38161 gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg cctgcttctg ccataacatg
    38221 cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg acgcccagca gaatacctgc
    38281 gataggcagt acggataccg gcagcatcag cgatttaccg accttttgca ggttagcaaa
    38341 tgcattctta aacataattg agagtgctcc tgagtatggg tgcttttttt acgttctcac
    38401 gcgtggcaag gggggagagc ctcgccgtgt acagggcatc taagcgccct ttatttatta
    38461 cacagagtaa aataattcag tgccaatttg tttgacggct atcacgtttc aacttcagat
    38521 cattggagaa gttgcacatt ttgacaaaac atatgtcaca atatttcaaa cgtggttatt
    38581 caccgcccaa ttttcggcgg tgaactttac tcgttttagc cattaattac gagctttaaa
    38641 aaaactcatt caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa
    38701 gttatcggtg gttacctgcg ccagttcttc aacggcaaca cctttcaaca cagccatgta
    38761 ttctgcaacg tcacgaacca tcgcaggttg attctctttt cctcgatgcg gaaccggtgc
    38821 aaggtaaggt gagtcagttt ctaccagtaa ccgatccagg gggacataac gcgcagcatc
    38881 gcgcagttgc tccgcattac ggaaggtcac aatgccggaa aaggagatgt aaaatccgag
    38941 atccagtaat ttacccgccg tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca
    39001 atccgtcact ttttcttcgc gcagaattgc cagcgtatcg gcgcgggcgt cacgggtatg
    39061 gacgataacc ggcttgttca gttcacgacc aatctggata tgatggatga aggactcttg
    39121 ctgccgtact ttagtttccg gcgtgtaata ataatccagc ccggtttcac ccagcgcaac
    39181 aacgccctct tctgccgcca gacggcgtaa atcttctaca tcgtagggat cattctggtt
    39241 gagtggatgc acgccacaag aaaatacgac gttgtcacgt tcgcctacca gatcccgcat
    39301 gtgtaaataa cccggtaatg ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc
    39361 ggctttcgcc agaacgtcat ccacatcctt atgcaaagat tcataatcca gaccatcgag
    39421 atggcagtgt gagtcgacta aaaacatgat gtctctctta aagatgagga accggtagca
    39481 caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg agaagctcgc
    39541 ggttaatacc tgtgacagac attaactgtt cacgaatgtg gcaaacatcc cccagtatag
    39601 cctgcaggcg cgagggagaa agatggtttg ccagttcgac gaccaggccc ggcacatcaa
    39661 cattggtcac ctgcgcagcg ccatgatggc gttttagcgc atccatcagc aacgttgcca
    39721 gccagtgtaa acgcgccgga gcttgttcat gattaagggc cgctagcagc gaataccagt
    39781 cgcccgattg cacgctatat gccagcgcct gacacaatgt ttcacgagcc tgccagttat
    39841 ctccctgaaa caacgccagt gccgccccag gcgaaccggc gcttaagcgc aatgcggcaa
    39901 gtagtgcatc ctgtgacatt gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg
    39961 gcggcggcgc aaggtaatgt aaccgacaac gactacgtaa tgttgccagt aaacgttcag
    40021 gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg ctcttcaagc gttttcagca
    40081 atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt tacccagaca actttcgcac
    40141 cacctaagcg tgcgtgctca tttaactttt cggtgacttc acgtaccgca tcaatgccca
    40201 gcgcattttt ccctttttcg ggagccaggg tgtaataatc gggatgcgtg ccagcctgca
    40261 tcaactgaca tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt tggcagagta
    40321 aataacggct cagggcgtag attaaagcat catcgcccat gcccggtaac gcctgaatga
    40381 gtagcgcatg gtgacctctt ccggcctgat agctggctac cagtttttcg aaatcaggtc
    40441 gtaaccatgg ataccatctc atgcgtccaa ctccttcacc cagttggtca cggtagtgcg
    40501 gattgcatcc atcacggcct ccagcggctg ggtggcatca atggtatgaa tgcttttatc
    40561 ttgtgctgcc agttccagat agcgggcgcg ggtgcgatta aagaaatcga aagactcttg
    40621 ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt aagccaacct ccggggtaac
    40681 atcgagatag agcgttaagt cggggcgaaa atccccgaga acagcatcac gcagtgttgc
    40741 cagcatatgt tggtcaatac cacgtcctcc accctgatag gcttgagtgg agagatcgtg
    40801 gcgatcgcca atcacccagg tgccgttagc cagcgcaggt ttgatgaccg tttctaccag
    40861 ttgaacgcgc gcggcataaa acatcagaac ttcggcttta tcggtaatga cttcatcgcc
    40921 taccgatttg atatccagca ccaggcttct taacttttcg gcaagttgcg taccgccagg
    40981 ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg agcgtctcaa ccaccacgtt
    41041 acgcgcggta gttttgcctg cgccttccag cccctcaatg acgatatact tactgcgcat
    41101 ttttttcctt aagcactttc agataatcct gcacagactt gttatgactg gcaagattgg
    41161 tattaaacgt gtgaccacct ttaccatcgg ccacaaaata gagatacggc gtttttgccg
    41221 gatgcgcagc agccttcagc gaatccgccc ccggcgtcgc tatcgcacct ggcggcagac
    41281 cggtaatggt ataggtgtta tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc
    41341 cattataacg ctctcccatc ccgtaaatca cggtcgggtc ggtctgcagg cgcataccaa
    41401 tgcgtaaacg gttgataaat actgaggcaa cctgatcgcg ttcactggca acggcggttt
    41461 ctttttcgat aattgatgcc atcgtcacca actggttttt atctttataa ggcagaccgt
    41521 ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga
    41581 gtaacgcgac atcggtggta ttggcggtat acatccaggt gtctggccag aaccaacctt
    41641 caatccactc cgggttttcc agttcaagtg cctgcgctac ggtggcgtac ttatcgtcgc
    41701 tcagcgtgtg cttgatatac ggagcctcac gcaattgctt gaggtagtcg ctcagacgca
    41761 tcccttctac cagtcgcagc gggaactgtg cttctttacc gctttccaac aatttcagca
    41821 tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat
    41881 ccggttcgat acgcagcaac cattgaaaaa cccgtgggcg attgatgatc ttatcggcat
    41941 aaagctgttc accgagcgcc agacgtccgg tccctggctt cagagtaaat atcgtctctt
    42001 ctttgataag caatttgctg tcggcaagat ggcgaacctt ccagacgccc acaccagcgg
    42061 cgatacccag taccaccagc aataacaaga ttatcaataa cacttttttc atgactaatt
    42121 cgggcgctca caaagtgggg ctaaatattc atataacgtt gctgacgaaa aggagacatc
    42181 gccacaggca cgtacgggca tcactggcat taacgcatta caaataacca tctcatctgc
    42241 ctgcaacgcc tcttccagag aggcttgcac ttcgacaagc tgataagagg attgtgccag
    42301 caaacggata cagaattgtc gcataatgcc gttaacacct gcctgatcca gtcgcggcgt
    42361 ataaactacg ttgcccttcc gccagaacaa attagccgca cagcattccg taacccaccc
    42421 ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc tcaagatgag agcggatcaa
    42481 tacttgctca agccgattaa gatgtttaat acctgcaaga tgaggattgc gccccagccg
    42541 caccgggctt agcgccaacg tcatcccttc gttacgcaaa cggtcgtaat gtgcaggata
    42601 agccgtaacg gagagaatcc gcgttgccgg tccactgttc aatgtgctgt accctcgccc
    42661 gccactaccg cgactgatca cgactttcag tacaccattt tgctgttctg ctgccagcgt
    42721 tttcatctct tgttcaagct gaggccagaa gtcacaggaa atcatcaacc gctgacaagc
    42781 atcctgtagt cgctggatat gcgccgataa caaactgact ttaccgtcga taactctggc
    42841 ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg cttactgcca gcgattcctg
    42901 cttatgaccg ttaattaaga acataccggc tccttatcag agaattgtta gtgtggcagc
    42961 atgttcacta cggaacaagt cggaataaaa actaagaaaa aaggcccgca agcggacctt
    43021 ttataagggt ggaaaatgac aatttagatc tttttaaaga tcaaagaacc attagtgcca
    43081 ccgaagccga aggagttaca cagagtgtat tccattccgc taacctgacg cgcttcgtgc
    43141 ggtacgaaat ccagatcgca accttcatcc gggttatcca ggttgatggt tggcggaaca
    43201 gcctgatcgc gcagcgccag gatggagtag atagattcta ctgcacccgc cgcacctaac
    43261 aggtgaccgg tcatagattt cgtggagctt accaacacac ggcttgcagc ttcaccgaag
    43321 atggttttca ccgcctgcgc ttcagcttta tcgccagccg gcgtagaagt accgtgcgcg
    43381 ttaacgtagc caatctgact cgcttcaatg cctgcatcac gcaaagcatt tgccattgcc
    43441 agagctgcgc ctgcgccatt ttctggcggt gacgtcatat gataagcatc gctgctcata
    43501 ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct
    43561 tcaagtacca gcataccggc accatcgccc agtacgaaac catcacgctc tttatcccac
    43621 gggcggctcg ccgcttgcgg gttatcattg cgggtagata atgcacgtgc cgcgccaaaa
    43681 ccaccaacgc ccagcggcgt actggctttc tctgcgccac ctgcaaccat cacgtcagca
    43741 tcgccatacg cgataatacg cgcagcatgg ccaatgttgt gcacgccgga agtacaggcg
    43801 gtcgcgatag agatgctcgg gccacgcagg ccatacatga tagtcagatg acctgccacc
    43861 atgttcacaa tcgttgacgg aacgaagaat gggctgatct tacgtggacc accgttcatc
    43921 agagatgtgt ggttttcttc gatcagtccg aggccgccaa tcccggagcc aattgcggca
    43981 ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag aatcctgcat ggcctgaacg
    44041 ccagcgacaa ttccatattg aatgaaggca tccatcttgc gctgttcttt gcgcgagata
    44101 atgtcctcac agttaaaatc ctttactaag ccagcaaatt tcgttgcata ggcgctagta
    44161 tcgaaatggt cgattaggct gatgccactc tgaccggcaa gcagagcttt ccaggtagac
    44221 tctacggtat tgccgacagg agacaacatg cccagtccgg tcacaactac acgacgctta
    44281 gacacgtttg tcctccaggg agggaaaaaa tgattctagt gggacaaaaa gataaaactc
    44341 aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt gatgtaatca
    44401 atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc agtatcaaac
    44461 tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc caggtcttca
    44521 acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc gccgataatt
    44581 ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa actcgctttc
    44641 gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc cggcaggtct
    44701 taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc attttgcact
    44761 aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc aaatcgcggt
    44821 cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta agctgcttcg
    44881 tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag gcgacccgca
    44941 ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt
    45001 tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc
    45061 agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc ctgaccgcca
    45121 tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg ctttttcatc
    45181 atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttggt ttcgataata
    45241 tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat accggcatta
    45301 ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac agattcgata
    45361 gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa ataatcactg
    45421 atcgcctgag cgccattttc actggtcgca gtgccgataa ctttcgcgcc acgggctgcg
    45481 agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag tgcgattttt
    45541 ccttcaaaat tcatgatttt cctcttttaa agctcgagcg ccgctgccat cgctgaaggt
    45601 tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc agtaagcact
    45661 ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta ttcaacagac
    45721 ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat ggcatcacca
    45781 ttggtttcgc atttcacatc aacgttattc acaacaggaa ctgttggtgc gttaaaggtg
    45841 attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc acagtgagac
    45901 ggtacgctca ctggtaacgg cagagcgcgt tttgcgcccg cagctttaca ggcagcgcca
    45961 gcacgctcaa ctgcttcttt atgaccggca ataaccacct gtcccggaga gttaaagttt
    46021 accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat agacgcatca
    46081 tccagaccga tgattgccgc catagcgccc gttccttccg gtacggcttc ttgcataaac
    46141 ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacacc agcgcaaacc
    46201 agcgcggagt attcccccag gctgtgacca gccatcattg ccggtgcttt accgccctgc
    46261 tgctgccata cgcgatacag cgcaacagat gcagtcaaca gcgctggctg ggtttgccag
    46321 gttttattca gttcttcagc tggcccctgc tgggtcagcg cccacaggtc gtagcccagc
    46381 gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc catatcagcc
    46441 agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg cgtcatgttt
    46501 taatccttat cctagaaacg aaccagcgcg gagccccagg tgaatccacc gccaaaggct
    46561 tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc atccagagcg
    46621 cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac gacattgtcc
    46681 atcgacatgc cgagtttttt cgccgttgca ctgataatac gcaggttagc ctgatgcgga
    46741 accagccagt ccagttgaga acggtcaaga ttattcgccg tcagcgtctc atcaacgatg
    46801 tgcgccagtt ctgttaccgc aaccttgaag acttcgttgc ccgccatcgt cagatgaatt
    46861 gaattctctg gattcacacg gtcagcatta ggcagcgtca gcaactcacc atagctaccg
    46921 tcggcatgca gatgggtgga gatgattccc ggctcttcag aggcagccag caccgcagcg
    46981 cccgcgccat cgccaaaaat aataatagtc ccacgatctg ttggatcgca ggtgcgcgcc
    47041 agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac gtattgatcg
    47101 gctacgctta atgcataggt gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa
    47161 cctttaatgc ccagcatgct ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca
    47221 gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc catctcaatt
    47281 gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg
    47341 cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac cattttttcc
    47401 aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat aatcttcgta
    47461 tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg cgagttcgct
    47521 ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct caaaaccagc
    47581 tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct gcaccgcctg
    47641 cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat gactttttat
    47701 caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt tgaggtgact
    47761 gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc accacgaccg
    47821 ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa caccttccat
    47881 cgtctttaat gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt
    47941 taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg ttttaagcac
    48001 cgctgaggca tcccgaatac tgtcgagacc ctttacttct tcttcaccaa tattgagcaa
    48061 cgccacgcga ggattgggaa tttccaccac ctcttcagcc aggactgagc ccataatggc
    48121 aaattgcacc aacattgtgc tgtcacaatc gacgttggcc cctaaatcga ggaccaccgt
    48181 tttgcccttt tgctgatgcg gtaataccgt caccagcgcc ggacgctcaa tcccctccag
    48241 gggcttgagt aataattttg ccagccccat cagcgccccg gtattaccgg cactgacaca
    48301 ggcttgcgct cgaccttctt tcaccagctc cagggccacg cgcattgaac tcccacgact
    48361 ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg caggaataat
    48421 ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga tggcgtcggg
    48481 attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca atgctgcagg
    48541 cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg ttagacgtgt
    48601 caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa atcctcacta
    48661 agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg gtagtaaccg
    48721 tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac agacaggctg
    48781 gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg ggttggttta
    48841 ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga tccaacgggg
    48901 agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg cttttgcgct
    48961 tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg ttcagaatca
    49021 tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc aagcagatcg
    49081 atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt
    49141 tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt aaacggcttc
    49201 ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa cactgcgaga
    49261 cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac actgactacg
    49321 gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc aaggcgtttt
    49381 tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat agggcgcgca
    49441 tattaacttt gtaacgtcat agagtcaaag aaaaaggcag catgtggttg ccttttgcca
    49501 ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat aacgaacatc
    49561 aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta tatctgatgg
    49621 aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc gctcttctgg
    49681 aaaaactgca aatctctttt gaatgtgcag caccagaggt cgacgaaacc ccacgcagcg
    49741 acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt
    49801 cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt gatggcgaaa
    49861 tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgtaaa gccagcggca
    49921 atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg catctgcaaa
    49981 cagaagtgga gccttttgac gtccatttcc gtcacctgag cgaggcggag attgataatt
    50041 acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa ggatttggca
    50101 ttacgctgtt tgagcgttta gaggggcgtg atcctaacac gctggttggt ttaccgctaa
    50161 tcgcgctgtg tcagatgtta cgtcgtgaag ggaaaaaccc gctgatggga tagttgtttg
    50221 cgccggatgc gtttacgtcg catccggcaa tcggtgctgg cagcctgatg cggcgtaaac
    50281 gccttatcag gccaaccgcc cattatcatg cttatatgct cgtattagcg cgcgttacgc
    50341 agtttttgca aacaacgctt caaaccatca tccatcggcg cttcgatacg catcacctca
    50401 ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg atttaatccc
    50461 gtgccggctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca
    50521 atcggatgac ccgcatactg tgtatgaaca cggatctgat gagtacgccc tgttaccggg
    50581 ctacaacgca ccagggtggc aaaagcatag cgttcttcca ctttaaagcg tgtttccgac
    50641 ggtttgcctt cctgactcac acgcacgata cgttcgccgc tttgcagaat atttttcagc
    50701 aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag cgccaggtaa
    50761 tctttttgca tccctttttc acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt
    50821 ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac
    50881 cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacac cgaagcttaa accactgccg
    50941 ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatggtcatc ttcatataag
    51001 atgacgtccg ccagcgctgc caccttttgc agatgtggcg aaaccgcctc ttcttcccgc
    51061 tcagcaacgc gaaccggtgg gatacgtact tcatcacccg cttcgagttt atattcaggc
    51121 ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta aatcatactt
    51181 tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca
    51241 gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggtggg cgattctaat
    51301 tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt gcaccttatt
    51361 acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca agaagtgaaa
    51421 aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca aggcttgcag
    51481 tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt tacggaataa
    51541 cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg atctttcagg
    51601 cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc tggagagtaa
    51661 tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg ggcaaccgac
    51721 actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact agacacgagg
    51781 ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa
    51841 taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg aagagttgcg
    51901 cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc cagggcacga
    51961 gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga gtctggaagc
    52021 tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag aaattgcccg
    52081 cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag atgtgttgcg
    52141 tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca aaggcgcggc
    52201 attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccga acaacccgcg
    52261 cgcgggtggc atttctcgtc gtatcgaagg cgacgaccgt accgaattaa aagaagcact
    52321 ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa
    52381 atctgctgag gcgctgcaat gggatttaag cttccgtctg aaacactggg aagccatcaa
    52441 aaaagccgct gaaagccgcc cggctccgtt cctgattcat caggagagca acgtaatcgt
    52501 tcgcgcattc cgcgattact tacgtcagga catcggcgaa atccttatcg ataacccgaa
    52561 agtgctcgaa ctggcacgcc agcatatcgc tgcattaggt cgcccggatt tcagcagcaa
    52621 aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg agtcacagat
    52681 tgagtccgcc ttccagcgtg aagttcgtct gccatccggt ggttccattg ttatcgacag
    52741 caccgaagcg ttaaccgcca tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat
    52801 cgaagaaacc gcgtttaaca ccaacctcga agctgccgat gagattgctc gtcagctgcg
    52861 cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc cagtacgcca
    52921 ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca
    52981 aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgtcagcgcc tgagcccatc
    53041 attgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta ccgtgcgtga
    53101 caacgaatcg ctgtcgctct ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa
    53161 cactcaggaa gttcacgcca ttgttcctgt gccaatcgcg tcttacctgc tgaatgaaaa
    53221 acgttctgca gtgaatgcga ttgaaactcg tcaggacggc gtgcgctgtg tgattgtgcc
    53281 aaacgatcag atggaaaccc cgcactacca tgtgctgcgc gtgcgtaaag gggaagaaac
    53341 tccaacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga
    53401 agaagagttc gctgaacgta agcgtcctga acaacctgcg ctggcaacct ttgccatgcc
    53461 ggatgtgccg ccagcgccaa ccccagctga acctaccgcg cctgttgtag ccccagcacc
    53521 taaagctgca acggcaacac cagcatctcc tgcacagcca gggttgttga gccgcttctt
    53581 cggcgcactg aaagcgctgt tcagcggtgg tgaagaaacc aaaccggccg agcaaccagc
    53641 accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa
    53701 ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa
    53761 tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg agacgcgtga
    53821 gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc agcaagcgcc
    53881 gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac aagaagcgaa
    53941 ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagagc gtgtacgtcc
    54001 ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt
    54061 agccgaagaa gcggtagtcg caccggtggt tgaagaaact gtcgctgccg aaccaattgt
    54121 tcaggaagcg ccagctccac gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca
    54181 agctgcacca gaacagcaag aagagaacaa tgccgataac cgtgacaacg gtggcatgcc
    54241 gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta
    54301 tcgtgacgag cgttatccaa cccaatcgcc aatgccgttg accgtagcgt gcgcatctcc
    54361 ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc aagatgtaca
    54421 ggtcgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga ctgaggtccc
    54481 tgtcgccgcc gctgtcgaac ctgttgttag cgcaccggtt gttgaagaag tggctgaagt
    54541 tgtagaagcc cccgttcagg ttgccgaacc gcaaccggaa gtggttgaaa cgacccatcc
    54601 tgaagtaatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt ctgatgttgc
    54661 cgtagcccag gaagttgcag aacacgcaga accggtggtt gaaccgcagg aagagacggc
    54721 agatattgaa gaagttgccg aaactgctga ggttgtggtt gctgaacctg aagttgttgc
    54781 tcaacctgcc gcgccagtcg tcgccgaagt cgcagcagaa gttgaaacgg tagccgcggt
    54841 taaacctgag gtcaccgttg agcataacca cgctaccgcg ccaatgacgc gcgctccggc
    54901 accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta cttttgcctt
    54961 cgaaggtaaa ggtgctgcag gtggtcatac ggcaacgcat catgcctctg ccgctcctgc
    55021 gcgtccgcaa cctgttgagt aataattagc tcaagtaatc aagccctggt aactgccagg
    55081 gcttttttat ttcagggtag catctttgtt gtaatgacat tagcgttaca tgcgctgtat
    55141 aattatcatc catgcaaagc cgaaggatat atttatgcca gcagccaata acattccaac
    55201 caggcgcgaa tctttaaatt tgcgcataaa acccgcagaa cgcgatctta ttgaccgggc
    55261 cgctaaagcc cgaggaaaaa atcgcaccga atttgtcctt gaggccgcca gagccgcagc
    55321 tgaagaggcg ctcattgagc agagaatttt caggatcgat tctgatgatt accagctatt
    55381 tttgaatcgt ctggatcagc cccccgcgcc caatgctgca ttacgcaaaa ccatgcaaac
    55441 acccgcgccg tgggagcaaa aaaaatgatt caggcccccg agccactcat ggcaaggcat
    55501 caattcacct ctttttgctc gggagtagaa acgatggata actggctaaa gcagagagcc
    55561 ctaaaaaatc agcttgccgg cgcatcacgt acatttgtta gctgtgatac gtatagcaac
    55621 gttttggcat attattcgct tgcttccagc gccgttgaaa cgtatgtcgc gacaggacga
    55681 tttcgccgaa atatgccaga accgatccct gttgtagtac ttggtcgtct ggcaatagat
    55741 aaatcattac agggtcaggg catcggtcgt gcgatggtca gagatgccgg tctacgagta
    55801 ctacaggcgg ctgaagtcat tggtattcgc ggaatgttgg tccatgcgtt atcagaccag
    55861 gcccgtgagt tttatctgcg agtggggttt gaaccatcgc ctgtcgattc tatgatactg
    55921 atggcaacat tggctgattt gcaggaatgt cttaaatagt tcgttatgtc tcatgcaaaa
    55981 aaatgcccgc cccggcgatc ccagagcagg cagagaaaac atacccagtt tcatgatatg
    56041 ttttaaagcg agcttatttg ttgagctgga acagcgacaa tccctgcata tcggtaaatg
    56101 ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc
    56161 agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta
    56221 atgaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga acagtcagca
    56281 cattgttcag cgagtttttc agtccgcggt tggttttatc cagtgccgca gcggcggttt
    56341 ctttatccgc ttcgctatcc gcgaccggcg tttttagggc tgcgatggcg ctatccagca
    56401 tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg ttgctggtaa
    56461 tactgtcgaa aattttgtca cccgtgtgcc ctatcaccat cgaacgcgaa gcatcgacct
    56521 gttgtttaat actttctgct ccgccttcgt atttgccatc cgcctcacta aacggcgcag
    56581 tttctgtttt ataaccggca aaaatgtagc gcccgttacc gtcagtggtg ttcgccagat
    56641 tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcacgg tcatcgtcac
    56701 tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata gcggtggtga
    56761 cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta cgcgccagcg
    56821 tgtactggct gttttgcgcc tgtgcctggg agagaacaac agcttgtgat gcagcaatgg
    56881 gatcatctga agggttaacg actcgcttac ccgtcgacat ctgttcgccg tacttcatcc
    56941 attctgcctg agaattggtg atgccgcgca tgttttgctg gtacatcatc tgggtactga
    57001 aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt
    57061 ctgcagaacc tgcgcattcg cgaggtaata ctgctgaaaa cgttgcagat ttccgtactc
    57121 ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt
    57181 accttgagtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa
    57241 cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactgt tgctttgcag
    57301 atcgagcagg gcctgaccgt tgcggttgtc gctatcaccc gcatcttctg cgctcgccat
    57361 ggcgattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttaccgg
    57421 tttcagtgtg aagctgtcgt taacggcagg cgttcctgtt aacgtcaact ccagaccatc
    57481 aaatgccact ttaccgttgg catccggcgt caccgtaaaa gtggtattac tggcaaggcg
    57541 ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccgcgga
    57601 ggcatcagtt accgttgcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac
    57661 cgcgggctta ccgatagcaa agaaatcttc accggcatcg ccgttagcat caaatccggc
    57721 tttgtgttgg gtgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg
    57781 cgtctggtca agatcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag
    57841 taatttttca gggatctcaa tattgcctgc cgtcccatca acataagcga cagtcgtacg
    57901 agaagggtcg gcgctggaag gaactgccgc aagttgccgc gccgtacttc cctgaaccag
    57961 tgagtagcca ttggccatcg tgatgttata agtgccacca tcctgaacgc tgacttctac
    58021 gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg
    58081 tgacgcccct gcccccacgc ctgtcaggcg ggagatttga tcgttcaggc tggcgatttg
    58141 tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc
    58201 ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc
    58261 aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt gcaggctggt
    58321 gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat
    58381 tttcgacatc tgctcatagc gggcagtcag gccgctactt tgcgtctgcg ccgcacgtaa
    58441 ctggttggta ataaacgcat catactcacg ctgcacacca gaaacgtaga cgccattgcc
    58501 aacccagccg ccagcgccca acgtgctatt ggcctgcgtc ataatagtgg tttggcgggt
    58561 atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc
    58621 cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc cttataagcc
    58681 ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta
    58741 tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta tcgatttcat ctgctgaatc
    58801 atgttggtga gtttgcgggc atagtgagga tcggtggcat agcccgcgtc ctgtagggcc
    58861 tgcgccccct gctccgcact cgcggcggtc gtcacggcgg cgtagcgcgg gttacgcgtt
    58921 aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg aaacttcgct
    58981 tttactttct tcgcttcgcc attttcatat tcagtcgtgg tgatttcagt gactggccct
    59041 ttccagttgt cagaggcttt gacgccaaac aggttatagc tcggctcgcc gttttcacgg
    59101 cggatttgcc gttgtcccca gccagactcc agcgccgcct gagcgaggat caaatgatgt
    59161 ggcacaccgc tttgctggct tgccagttgg gcgggcaacg agagttgcgc gaggaatgct
    59221 ttactgtcac ccggcagcga atcatcgtag ttacgtggta cggccttttg caccagctgc
    59281 gaaagcgtct gattttgata acgcaccacg gtttcgagcg ggaatttcat cggtgctgct
    59341 ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgcc
    59401 agccccagac ctttgcccgc cgtcatctgt tgggcaatct gctggtcata catactggta
    59461 tacaggcgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt
    59521 ttcaacatca tctgcacgaa catcccttcc acctggcggg ccaccggacg gatatttgcc
    59581 gccggatctt cgcccgcttt cgccttaagt tcgttgagcg attgtgcatc ccaggccgca
    59641 cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc
    59701 ccgcactttg cattgattgc agtatggaca tcagatccat cggcgtagcg cccagcgcat
    59761 tgagcgcacg caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc
    59821 cgctctggcg taaatcgatc tgcatttgtg gagtaaccac agtctgccca ccaccaaacg
    59881 gtgtatctgg ctggctgaca ttggcctggc gattaactgt tactgagaga ttcccctgcg
    59941 ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaacca gtgcgcgagt
    60001 taatcactac tttagcgtcc tgcggggtaa cattaacctg catattctgg atatcggcaa
    60061 ggaaacggac ctgggaactg ttgccactcg gtacgcgcac ctgaatagtc cgcgcatcta
    60121 acgcggtggc gctgccatat ccacgcacac ggttgatggt gtcagcgatt tgctgcgcca
    60181 tactgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg
    60241 gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttcacct
    60301 gaacactgct accgccagcg gaggcccctg cgccgccaac cagaatattg ccctgcgcca
    60361 gcgcatacac ctgactgtca acgcccttaa gcggtgtcat caataacgta ccgccacgca
    60421 gacttttggc atttcccatg gaagaaacta ccacgtcaat ggtttgcccc tgacgtccaa
    60481 acggtggaag tgacgctgtc accataaccg cagcgacgtt ttttagctgc atattagtgc
    60541 ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg
    60601 gcgtctgagt tgtctggtca cctgtaccat caagccccac caccaggcca tagccaatca
    60661 gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg
    60721 ccgtggtaac cagtaaaaga attaatgcag agagaaattt aatcaccaca gcctcactta
    60781 cattggtgac aggttgagga agaaacgctg caaccagccc atattttgcg cttcgttaat
    60841 gtagccattg cctacgtatt caatacgtgc atccgccacc tgagtagacg gtacggtatt
    60901 gctgccgctg atagtgcgtg gattaactac accagagaag cgaataaatt cggtaccctg
    60961 attaatggcg atctgttttt cacccaccac atgcagattg ccgttgacca gtacctggtc
    61021 aaccgtcacc gtcaacgtgc cgctaaaggt attactggca ttggccccgc cttttccgtt
    61081 gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa
    61141 atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga
    61201 ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt
    61261 gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg
    61321 gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc
    61381 ctgtaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca ccaacaagct
    61441 ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta
    61501 aaccggtgaa tctgccaccg gttatgcctt agagttgcgt cagtttttgc agcatctgat
    61561 cggtggtgga taccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga
    61621 ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg
    61681 cgccgttcag gcccggcgtg ctttcgttcg gtgcaccgga ggattgcgtt tcggtgtaga
    61741 ggttttcgcc aatactctcc agtccggtat cattcataaa ggtggtgaga ttgagctgcc
    61801 caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga
    61861 tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag
    61921 ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    61981 agccatctgg caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    62041 tattggtttg cgacaggttg ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    62101 ccgtaccgat ttgtaatccg gatggtaagg tggtttgttc ggaagactgt gcccccggct
    62161 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttgg
    62221 tactaacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcatccaggc
    62281 ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaag
    62341 cagagcagaa gcacgcgacg gtgcgaattt cccctcaccc taacctctcc caaaatgggc
    62401 gagagaatag tccgcgcgct tttattgcaa tgtcatcaat cgtttaatta actcatcgac
    62461 aacagttggt tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt
    62521 tcaaaacgcc gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg
    62581 ccttccagaa ctcccgacat cacacgcaag gttggatctg cctgcagtac cggcccacgc
    62641 gtggcctggc tttccgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg
    62701 cccgtggctt tcaccagttt cagacgcccc actggcgcaa ccgtatttgc cggatcgccc
    62761 ggattcagcg ccgagattgt gccatcggca gcaatagtga tttccgcccc ttccggcaca
    62821 gcaattggcc ccgcctcgcc tatcaccgga tgcccctgaa ttgtcagttg cccggtggga
    62881 tcaacctgaa tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc
    62941 agccagccat cctgctgcaa cgcaacgtcc agcgggcgcg aggtgtaatc catcttgccg
    63001 ggcgtcatgt ctgcacccgg cgttgacgcc gttaccagcg tgcgcgtggg cagtgaaagc
    63061 ccttccaccg gcactgcgcg caaggcgttt aactgcgcgc gaaaaccggg cgtagaggca
    63121 ttggccagat tactggcggt taccgcctgt tgattcaacg tctggctggc tgctcccatc
    63181 gcggtataaa ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag
    63241 cgtgttgagg atctggtcct gggttttgat ggtctgggcg ttagactgat agttacgctg
    63301 ggcaacgatc atattgacca gttctttact gagatcgacg ttggacgctt ccagcgcacc
    63361 gttggtcagg gtgccaaagt tgcccgtccc ggccgtcccc aacagcgcca cgccagaaga
    63421 ttgcgtcgca gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt
    63481 cgccagtaca atctgcccca gcagttgggt ttgttcgttg gaatagttgc cgacaaccgt
    63541 accgtcatca ttgatttgat aactcaccag atcgcccggt ttgtagccgt tctgggtggt
    63601 taccacaatg ttgttagcac cggtattttg ctgcatggag ttgaggaagc tcaggctaaa
    63661 cgtggcggga tctgcgccgt taattgcgcc agtggtaata ttcgctgttg gatttgaggt
    63721 cagaacacca ttggcattaa acaccagcgt cattgcatgg tcagctgtgc ctgttggatc
    63781 actactatcc tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct
    63841 catgtcatga gcattaccct gactatcgaa aacagtcacc gaaccttttt tgttatagct
    63901 atccgcattg ctggcatcaa atgcgttaac agagggaagc ggatcactgg aattcaggtt
    63961 gatctgcatc gacgccgtgg tggtagtttt cgctgccatc agggtattcg gaatcgaaat
    64021 attggtcgga ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt
    64081 cagctgtaaa ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt
    64141 acggctgtag aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat
    64201 agcaacgtcc agacctcgcc cggtgttggt agtcgtgcca tcggtaaagt cctgggtgat
    64261 accggcaact tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc
    64321 cgtgcctgat ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag
    64381 gttggtggcg gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg
    64441 aaatgtgaag gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc
    64501 gagatccagc gtattaccgt tgttgccgcg gatcacaccc tgcaccagag caaactgcag
    64561 cggctgggca accagttgtg taccaccgtt actggcgcta atcgctacgt tgtaagaacc
    64621 gttcggcgca gtggtgccgt cagtcaacgt accgtcccag gtgaaactgt ggactccggc
    64681 ggtcagttca ccaatatcaa tggtacggac aaccgcgcca tttttatcgg tgatggtggc
    64741 cgtaactttg tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc
    64801 cccttcttca ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc
    64861 gatcaggtta ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag
    64921 cgtggtattg agtttttcaa tcccgctgac cgtgctgatt tgagccaatt gcgacgtcag
    64981 ctcgttgttt tccattggat tggtcgggtc ctggtttttc agctgcgcca ccagcaaagt
    65041 cagaaaactg ctttgtaaat ctgcggcgtt gctgcccgtg agcgaactac tactggtggt
    65101 gctgacgccg gtatttgtcg gatcggtggt ggttaccgca atggacatag ttttctcctt
    65161 tattgaccga gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca
    65221 ttggcctgat agctgcgtga cgccgacatg gtgttaacca tctctccgac gacatcaacg
    65281 tttggcattt tcacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt
    65341 ttgtccgggg cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct
    65401 ggtgcagcgt taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg
    65461 gtcacgctat cagcattcgc cagattactg gccgccacgt tcaggcgctg ggactgggca
    65521 gttaacgccg acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc
    65581 tgtaaaacgt tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat
    65641 tgcaggctgt tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca
    65701 agcgaaggct ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc
    65761 gccggaatgt gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca
    65821 cgttgcatga cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg
    65881 gtatcggcat tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc
    65941 gcctcttgtt gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta
    66001 tcaaaattct gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca
    66061 aaatgggtcg ctatttatgc cgttgatggt cattgcggac aggtacaatt cacgttgtag
    66121 aaatggcagg aggcgaaaat gctggcaata aaacgtagcg tggcgatcat cgcgatactg
    66181 ttcagtccgt taagtgcggc gagcaatctc gcatcgcaat tgcacacctt ttttagcgcc
    66241 cagctcgcgg gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta
    66301 ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg
    66361 aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt gcaggccaca
    66421 ggaaattatg tggttgccgc aatgcccatt gtgcggggag gaaagctgga agctggcaat
    66481 gtcaaactga aacgcggacg gctggacacc ttgccaccgc gtacggtgct ggatatcaat
    66541 caacttgttg atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc
    66601 cagtttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagtggt
    66661 gatgggttta gcgccaacgc agaaggtcag gcgctgaaca atgctgccgt cgcacagaat
    66721 gcgcgggtgc gcatggtatc gggacaggtg gtcagcggcg ttgttgatgc agatgggaat
    66781 attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca
    66841 catgataaaa gcgccctcaa tgaggaataa acaatgagta ttgatcgcac ttcgcctctg
    66901 aagcccgtaa gcaccgttca accgcgcgaa accactgacg cgccggtaac gaacacccgg
    66961 gcggcaaaaa caaccgcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa
    67021 ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg
    67081 attcgtaacg gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa
    67141 gcgcagcaag acttgcagag taactgatcg tatgacgcgt cttgcagaga tcctcgacca
    67201 gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc aacatctctc
    67261 tatggggcag atcaacggca gtcagttgca atggattaca gaacaaaaaa gctctctgct
    67321 ggcgacgctg gattacctcg aacagttacg caggaaagaa cccaattcag caaatagcgt
    67381 tgatattagt caacgctggc aggcaattac tgtgaaaacg cagcaactac gccaaatgaa
    67441 tcaacataac ggctggttac tggaaggaca gattgagcgc aaccaacaag cactggaaat
    67501 gttgaaaccg catcaggaac cgacgctata tggggcaaac ggtcagacct caacaaccca
    67561 tcgcggcagt aaaaagattt cgatctgaag attatctccg gcctgcactg caggccggaa
    67621 tgcattgtta caccgtccgg cgggcaaact ctttaacttt gaagcccagt accgccagtg
    67681 cagcaaagta cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg
    67741 gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc acgcccgaca
    67801 tcaccagtac cgccactacc agacgcaaca gaaacgccat ccagccaggt tgcggggtaa
    67861 agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac
    67921 caatagaaag tgacagcccg gcatgtttca acggaccgat aaacgccagg ttcatcaatt
    67981 gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat
    68041 aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa
    68101 ttaacgcccg ctgggtcatc agcgcatcaa aggcggtaaa tttaccgtac tggaacagcg
    68161 aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagc gccaacagga
    68221 aacaaagacg cagcccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa
    68281 aacttttcga cagcgacggc agcaaaatgg tgccaagcgc cacgcccagt acaccggacg
    68341 gaaactccat taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg
    68401 aggcaaaaat ggtgttgatg attaaggaga tctgactcac agagacgcca aggatcgccg
    68461 gtcccatctg tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga
    68521 ccagcatgcc gatcttcttc agatgcggta gctgatacac cagctgcagg atgccgccca
    68581 ccgtaacagc ccacgccagc gccagcaccg gtggattaaa gtacggtgcg gcaaacagcg
    68641 caaagccaat catgctgata ttgagcaatg tcggggcaaa cgccggaatc gagaagcggt
    68701 tccacgtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa
    68761 aggtaatctt tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg
    68821 gcgcggtcac catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca
    68881 gcgcaagtgt cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct
    68941 gcttactttt atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa
    69001 agatacggcg taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc
    69061 ctgcgccaaa gattctggcg acaattgcgt cacgtgcgaa gccaagcaca cgcgaaaaca
    69121 tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga
    69181 cccacgggca aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta
    69241 tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc
    69301 ttgtcaacga tacgttgcgc cagcactgcc tgttcgccgg cggtttgcgg aactgtctga
    69361 ttttgcacac attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagcgta
    69421 ctctgccaac caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg
    69481 cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctgg
    69541 cttccggcac ggcgatgcat acaggtggtg atctgcaaag gaccggccga aaaatggtgc
    69601 tcggcaaaca gcatttcgcc agcgtcatta gtcagtagcg taccgccatc cagagaggct
    69661 ttaccgcccg acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg
    69721 aaataaagat cgtgtggccc gacgctattg ctacgatgtt tatccattct tagcgaggct
    69781 gcggtggcga gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg
    69841 accatcaacg tcagtttttt ccgtgccgca agttccacca gccgttcagc atcgcgcaga
    69901 ttttctgcca gcggtttatc gacacagaca tgtacccccg cattgagtaa cgtactgacc
    69961 acgtcaaagt ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg
    70021 ctggataacg aatcggcata aggaatgcgc cagctgtcac aaattggcaa ggctttcgcg
    70081 cgcgtaggcg accacgcacc ttgtaacgtc cagtcagacg ctgccgccag caccggtaac
    70141 cacgcctttt gcgcgatgcc gcctaacccc actacgccga tacgtaattt tttcacttta
    70201 atctcccaga tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc
    70261 cagggcttca acgcgtgcct gtaaatcacc gtcaaccgcg ttcgacatat ccatcaccgc
    70321 cggctgatct tcaacctcac cactgaaaag atgcatgtag cggctttcac gtttacccgg
    70381 ttcgcgggcc agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt
    70441 cgactccact tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc
    70501 acccggcgtc tgggcaccac gcaataacaa cgtggtgatt aacgccactt ctgcggcgct
    70561 cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg tgacccgatt
    70621 accaaaaccg ctcactgtgc gtagataatg acgtttgacc agattatcca gctgttcctg
    70681 cacttcggat tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt
    70741 gactacacca ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa
    70801 gcaaccaatc acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc
    70861 ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat
    70921 caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat
    70981 caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc
    71041 gctgcatata acgaatggct gcgctcaggg cttcaaacat gagtcctttg ccctgccatt
    71101 tttgcccaat cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa
    71161 aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg
    71221 aaccttgttt atgaaattcg ttaatcatcc ctagcctggc ctgccagcct gatggataac
    71281 agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat
    71341 aatccgccag acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg
    71401 ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg
    71461 taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt
    71521 ccagaatgcc attttctgca ttattcacat atttgataaa aactctctat caaagcgacg
    71581 ttttgataaa aaaatattgt ccccgaaatg atgggaaaag tcttagcggc aatgcagaat
    71641 agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtgtcg caggccagga
    71701 acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg
    71761 tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg
    71821 gtattgccct cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa
    71881 ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgtgccgccg
    71941 gattcgccac aatgggtatc gcccacgaac cgtggctact gtggttttca tgcctgctct
    72001 cgggactcgg cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc
    72061 gtccacaaca gcgtggtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg
    72121 tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca
    72181 caggggcagt tttgtttgtg ctgtgtgcag cgttcaatgc gtggttgtta ccggcatgga
    72241 aactctccac tgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc
    72301 gttttgtcac ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc
    72361 tgatgctgcc aattatggtc aacgacgtgg cgggcgcgcc ctctgccgtt aaatggatgt
    72421 atgccattga agcgtgtctg tcgttaacgt tgctctatcc tatcgcccgc tggagtgaaa
    72481 agcattttcg tctggaacat cggttgatgg ctgggctgtt gataatgtca ttaagcatga
    72541 tgccagtggg catggtcagc ggcctgcaac aacttttcac cctgatttgt ctgttttata
    72601 tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcagacgcaa
    72661 gagctcgcgg cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg
    72721 gttatatcgg tggcggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc
    72781 cgtggatgat gctgggcatt attggcatct tcactttcct tgcgctgggt tggcagttta
    72841 gccagaaacg cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct
    72901 gactcctcca tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca
    72961 tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc
    73021 ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc
    73081 aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggaca
    73141 tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg
    73201 ccattgaaga gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt
    73261 ttgccaaatc ggcatttgat gagttttcta ctcctgccgc gcgcaaatat ttcgtcgaca
    73321 agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta
    73381 agaatatcag cgatgattta cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga
    73441 atggcgaact ttcggaagat gatattcagc tatttccgct cctgcgtaat ctgacgctgg
    73501 tagccggaat caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga
    73561 cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta
    73621 ttcgcgggtt gccgccgttt tcagtttttt ctgctggcgc gtttatcgct ggtgacacat
    73681 gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt
    73741 ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa
    73801 tacaccatca ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa
    73861 gatatcggtt tacccggcgt ggctgacgcc catattgttc tgacaaacct gaccagccaa
    73921 attggtcgcg aagagccgaa taaggttacc ttaaccggag acgccaatct ggacatgaac
    73981 tccctgttcg gtagccagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt
    74041 gataaagaaa aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa
    74101 ccggaaaaaa tgcaaacggt gatgcaaacg ttgctcccct atttgaacca ggcattacgc
    74161 aattacttta accagcaacc agcttacgtc ctgcacgaag atggcagcca gggcgaagca
    74221 atggcgaaga aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt
    74281 acggattaat cacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc
    74341 cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact
    74401 gcaccatccc aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat
    74461 ggcgacatgt taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta
    74521 atgcccaatc tggctccgcc cgtgaccacc gttgaggctg ccgtggctta tcgccagcgt
    74581 attcttgacg ccgtacctgc cgggcacgat ttcagcccgc tgatgacctg ttatttgaca
    74641 gattcactag accctaatga gcttgagcgt ggatttaacg aaggcgtgtt caccgctgcc
    74701 aaactttacc cggcaaacgc aaccactaac tccagtcacg gcgtgacgtc agttgacgca
    74761 atcatgccgg tacttgagcg catggaaaaa atcggtatgc cgttactggt gcatggtgaa
    74821 gtgacacatg cagatatcga catttttgat cgtgaagcgc gctttattga aagcgtgatg
    74881 gaaccgctgc gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa
    74941 gacgctgccg actatgtccg taacggaaat gaacggctgg ctgccaccat cactccgcag
    75001 catctgatgt ttaaccgcaa ccatatgctg gttggtggcg tgcgtccaca cctgtattgt
    75061 ctccccatcc tcaaacgcaa tattcaccaa caggcactgc gtgaactggt cgccagcggt
    75121 tttaatcgcg tattcctcgg tacggattct gcgccgcatg cacgtcatcg caaagagagc
    75181 agctgcggct gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc
    75241 tttgaagaga tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccacag
    75301 ttctatggct tgccggtcaa cgacacattc atcgaactgg tccgcgaaga gcaacaggtt
    75361 gctgaaagca tcgcattgac tgatgacaca ctggtgccat tcctcgccgg ggaaacggta
    75421 cgctggtccg ttaaacaata aaaatcctag cttcctgttg tcattaggtt at
//
LOCUS       DAYPHN010000005        73035 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000005 DAYPHN010000000
VERSION     DAYPHN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.005"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(13..510)
                     /gene="rplJ"
                     /locus_tag="ACG0BW_000311"
     CDS             complement(13..510)
                     /gene="rplJ"
                     /locus_tag="ACG0BW_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFK7257327.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(802..1506)
                     /gene="rplA"
                     /locus_tag="ACG0BW_000312"
     CDS             complement(802..1506)
                     /gene="rplA"
                     /locus_tag="ACG0BW_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFK7257328.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(1510..1938)
                     /gene="rplK"
                     /locus_tag="ACG0BW_000313"
     CDS             complement(1510..1938)
                     /gene="rplK"
                     /locus_tag="ACG0BW_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFK7257329.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(2097..2642)
                     /gene="nusG"
                     /locus_tag="ACG0BW_000314"
     CDS             complement(2097..2642)
                     /gene="nusG"
                     /locus_tag="ACG0BW_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFK7257330.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(2644..3027)
                     /gene="secE"
                     /locus_tag="ACG0BW_000315"
     CDS             complement(2644..3027)
                     /gene="secE"
                     /locus_tag="ACG0BW_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFK7257331.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(3257..4441)
                     /gene="tuf"
                     /locus_tag="ACG0BW_000316"
     CDS             complement(3257..4441)
                     /gene="tuf"
                     /locus_tag="ACG0BW_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFK7257332.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            complement(4512..6626)
                     /gene="fusA"
                     /locus_tag="ACG0BW_000317"
     CDS             complement(4512..6626)
                     /gene="fusA"
                     /locus_tag="ACG0BW_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFK7257333.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(6723..7193)
                     /gene="rpsG"
                     /locus_tag="ACG0BW_000318"
     CDS             complement(6723..7193)
                     /gene="rpsG"
                     /locus_tag="ACG0BW_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFK7257334.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(7290..7664)
                     /gene="rpsL"
                     /locus_tag="ACG0BW_000319"
     CDS             complement(7290..7664)
                     /gene="rpsL"
                     /locus_tag="ACG0BW_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFK7257335.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(7790..8077)
                     /gene="tusB"
                     /locus_tag="ACG0BW_000320"
     CDS             complement(7790..8077)
                     /gene="tusB"
                     /locus_tag="ACG0BW_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFK7257336.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(8085..8444)
                     /gene="tusC"
                     /locus_tag="ACG0BW_000321"
     CDS             complement(8085..8444)
                     /gene="tusC"
                     /locus_tag="ACG0BW_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFK7257337.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLQGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(8444..8830)
                     /gene="tusD"
                     /locus_tag="ACG0BW_000322"
     CDS             complement(8444..8830)
                     /gene="tusD"
                     /locus_tag="ACG0BW_000322"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFK7257338.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQSGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(8830..9552)
                     /gene="yheO"
                     /locus_tag="ACG0BW_000323"
     CDS             complement(8830..9552)
                     /gene="yheO"
                     /locus_tag="ACG0BW_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7257339.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(9719..10531)
                     /gene="fkpA"
                     /locus_tag="ACG0BW_000324"
     CDS             complement(9719..10531)
                     /gene="fkpA"
                     /locus_tag="ACG0BW_000324"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFK7257340.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            10752..10970
                     /gene="slyX"
                     /locus_tag="ACG0BW_000325"
     CDS             10752..10970
                     /gene="slyX"
                     /locus_tag="ACG0BW_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFK7257341.1"
                     /translation="MQDLSFEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(11019..11609)
                     /gene="slyD"
                     /locus_tag="ACG0BW_000326"
     CDS             complement(11019..11609)
                     /gene="slyD"
                     /locus_tag="ACG0BW_000326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFK7257342.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(11704..11904)
                     /gene="yheV"
                     /locus_tag="ACG0BW_000327"
     CDS             complement(11704..11904)
                     /gene="yheV"
                     /locus_tag="ACG0BW_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFK7257343.1"
                     /translation="MAIRKRFIASAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(11914..13719)
                     /gene="kefB"
                     /locus_tag="ACG0BW_000328"
     CDS             complement(11914..13719)
                     /gene="kefB"
                     /locus_tag="ACG0BW_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFK7257344.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMRDKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVIAVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(13719..14270)
                     /gene="kefG"
                     /locus_tag="ACG0BW_000329"
     CDS             complement(13719..14270)
                     /gene="kefG"
                     /locus_tag="ACG0BW_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFK7257345.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            14401..16314
                     /gene="yheS"
                     /locus_tag="ACG0BW_000330"
     CDS             14401..16314
                     /gene="yheS"
                     /locus_tag="ACG0BW_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7257346.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNELSADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLRDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            16314..17336
                     /gene="yheT"
                     /locus_tag="ACG0BW_000331"
     CDS             16314..17336
                     /gene="yheT"
                     /locus_tag="ACG0BW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFK7257347.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            17330..17548
                     /gene="yheU"
                     /locus_tag="ACG0BW_000332"
     CDS             17330..17548
                     /gene="yheU"
                     /locus_tag="ACG0BW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFK7257348.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            17602..18471
                     /gene="prkB"
                     /locus_tag="ACG0BW_000333"
     CDS             17602..18471
                     /gene="prkB"
                     /locus_tag="ACG0BW_000333"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFK7257349.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(18526..18930)
                     /gene="yhfA"
                     /locus_tag="ACG0BW_000334"
     CDS             complement(18526..18930)
                     /gene="yhfA"
                     /locus_tag="ACG0BW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFK7257350.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            19232..19864
                     /gene="crp"
                     /locus_tag="ACG0BW_000335"
     CDS             19232..19864
                     /gene="crp"
                     /locus_tag="ACG0BW_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFK7257351.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            19915..22005
                     /gene="yhfK"
                     /locus_tag="ACG0BW_000336"
     CDS             19915..22005
                     /gene="yhfK"
                     /locus_tag="ACG0BW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFK7257352.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNNDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(22072..23292)
                     /gene="argD"
                     /locus_tag="ACG0BW_000337"
                     /gene_synonym="dapC"
     CDS             complement(22072..23292)
                     /gene="argD"
                     /locus_tag="ACG0BW_000337"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFK7257353.1"
                     /translation="MATEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDIHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRTRDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(23378..23941)
                     /gene="pabA"
                     /locus_tag="ACG0BW_000338"
     CDS             complement(23378..23941)
                     /gene="pabA"
                     /locus_tag="ACG0BW_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFK7257354.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRNYAGCLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(23973..24575)
                     /gene="fic"
                     /locus_tag="ACG0BW_000339"
     CDS             complement(23973..24575)
                     /gene="fic"
                     /locus_tag="ACG0BW_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFK7257355.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEDGYLVGLDKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(24565..24732)
                     /gene="yhfG"
                     /locus_tag="ACG0BW_000340"
     CDS             complement(24565..24732)
                     /gene="yhfG"
                     /locus_tag="ACG0BW_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFK7257356.1"
                     /translation="MKKLTDKQKSRLWEFQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(24837..25409)
                     /gene="ppiA"
                     /locus_tag="ACG0BW_000341"
                     /gene_synonym="rotA"
     CDS             complement(24837..25409)
                     /gene="ppiA"
                     /locus_tag="ACG0BW_000341"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFK7257357.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDIAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            25680..26861
                     /gene="tsgA"
                     /locus_tag="ACG0BW_000342"
     CDS             25680..26861
                     /gene="tsgA"
                     /locus_tag="ACG0BW_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFK7257358.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSIAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            27123..29666
                     /gene="nirB"
                     /locus_tag="ACG0BW_000343"
     CDS             27123..29666
                     /gene="nirB"
                     /locus_tag="ACG0BW_000343"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFK7257359.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVHVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            29663..29989
                     /gene="nirD"
                     /locus_tag="ACG0BW_000344"
     CDS             29663..29989
                     /gene="nirD"
                     /locus_tag="ACG0BW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFK7257360.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            30115..30921
                     /gene="nirC"
                     /locus_tag="ACG0BW_000345"
     CDS             30115..30921
                     /gene="nirC"
                     /locus_tag="ACG0BW_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFK7257361.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            30940..32313
                     /gene="cysG"
                     /locus_tag="ACG0BW_000346"
     CDS             30940..32313
                     /gene="cysG"
                     /locus_tag="ACG0BW_000346"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFK7257362.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEAHRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            32565..32732
                     /gene="yhfL"
                     /locus_tag="ACG0BW_000347"
     CDS             32565..32732
                     /gene="yhfL"
                     /locus_tag="ACG0BW_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFK7257363.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(32839..33924)
                     /gene="yhfS"
                     /locus_tag="ACG0BW_000348"
     CDS             complement(32839..33924)
                     /gene="yhfS"
                     /locus_tag="ACG0BW_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFK7257364.1"
                     /translation="MKTYPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTRGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQVNPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(33936..35240)
                     /gene="yhfT"
                     /locus_tag="ACG0BW_000349"
     CDS             complement(33936..35240)
                     /gene="yhfT"
                     /locus_tag="ACG0BW_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFK7257365.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNLMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFVPA"
     gene            complement(35252..35605)
                     /gene="yhfU"
                     /locus_tag="ACG0BW_000350"
     CDS             complement(35252..35605)
                     /gene="yhfU"
                     /locus_tag="ACG0BW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFK7257366.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(35616..36494)
                     /gene="php"
                     /locus_tag="ACG0BW_000351"
     CDS             complement(35616..36494)
                     /gene="php"
                     /locus_tag="ACG0BW_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFK7257367.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMHATGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAFVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(36491..37717)
                     /gene="yhfW"
                     /locus_tag="ACG0BW_000352"
     CDS             complement(36491..37717)
                     /gene="yhfW"
                     /locus_tag="ACG0BW_000352"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFK7257368.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERGGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLLDSQRILDAAESKEERFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(37717..38880)
                     /gene="yhfX"
                     /locus_tag="ACG0BW_000353"
     CDS             complement(37717..38880)
                     /gene="yhfX"
                     /locus_tag="ACG0BW_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFK7257369.1"
                     /translation="MFVEALKRQNPALISAALSLWQKGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVVVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAWEVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKALPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(38964..39326)
                     /gene="yhfY"
                     /locus_tag="ACG0BW_000354"
     CDS             complement(38964..39326)
                     /gene="yhfY"
                     /locus_tag="ACG0BW_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFK7257370.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNLLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDAELLAELAQSSHWQAVVQLHQVLLKEFTLEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(39343..40248)
                     /gene="yhfZ"
                     /locus_tag="ACG0BW_000355"
     CDS             complement(39343..40248)
                     /gene="yhfZ"
                     /locus_tag="ACG0BW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFK7257371.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(40408..41412)
                     /gene="trpS"
                     /locus_tag="ACG0BW_000356"
     CDS             complement(40408..41412)
                     /gene="trpS"
                     /locus_tag="ACG0BW_000356"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFK7257372.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(41405..42163)
                     /gene="gph"
                     /locus_tag="ACG0BW_000357"
     CDS             complement(41405..42163)
                     /gene="gph"
                     /locus_tag="ACG0BW_000357"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFK7257373.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(42156..42833)
                     /gene="rpe"
                     /locus_tag="ACG0BW_000358"
     CDS             complement(42156..42833)
                     /gene="rpe"
                     /locus_tag="ACG0BW_000358"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFK7257374.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(42851..43687)
                     /gene="dam"
                     /locus_tag="ACG0BW_000359"
     CDS             complement(42851..43687)
                     /gene="dam"
                     /locus_tag="ACG0BW_000359"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFK7257375.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFIPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(43794..45080)
                     /gene="damX"
                     /locus_tag="ACG0BW_000360"
     CDS             complement(43794..45080)
                     /gene="damX"
                     /locus_tag="ACG0BW_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFK7257376.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(45172..46260)
                     /gene="aroB"
                     /locus_tag="ACG0BW_000361"
     CDS             complement(45172..46260)
                     /gene="aroB"
                     /locus_tag="ACG0BW_000361"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFK7257377.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(46317..46838)
                     /gene="aroK"
                     /locus_tag="ACG0BW_000362"
     CDS             complement(46317..46838)
                     /gene="aroK"
                     /locus_tag="ACG0BW_000362"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFK7257378.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(47239..48477)
                     /gene="hofQ"
                     /locus_tag="ACG0BW_000363"
     CDS             complement(47239..48477)
                     /gene="hofQ"
                     /locus_tag="ACG0BW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFK7257379.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVRGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(48389..48793)
                     /gene="hofP"
                     /locus_tag="ACG0BW_000364"
     CDS             complement(48389..48793)
                     /gene="hofP"
                     /locus_tag="ACG0BW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFK7257380.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGIV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDTDGG"
     gene            complement(48783..49223)
                     /gene="hofO"
                     /locus_tag="ACG0BW_000365"
     CDS             complement(48783..49223)
                     /gene="hofO"
                     /locus_tag="ACG0BW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFK7257381.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPDEG"
     gene            complement(49207..49746)
                     /gene="hofN"
                     /locus_tag="ACG0BW_000366"
     CDS             complement(49207..49746)
                     /gene="hofN"
                     /locus_tag="ACG0BW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFK7257382.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(49746..50525)
                     /gene="hofM"
                     /locus_tag="ACG0BW_000367"
     CDS             complement(49746..50525)
                     /gene="hofM"
                     /locus_tag="ACG0BW_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFK7257383.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGTKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGITSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            50645..53197
                     /gene="mrcA"
                     /locus_tag="ACG0BW_000368"
     CDS             50645..53197
                     /gene="mrcA"
                     /locus_tag="ACG0BW_000368"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFK7257384.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(53365..53925)
                     /gene="nudE"
                     /locus_tag="ACG0BW_000369"
     CDS             complement(53365..53925)
                     /gene="nudE"
                     /locus_tag="ACG0BW_000369"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFK7257385.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            54245..56380
                     /gene="igaA"
                     /locus_tag="ACG0BW_000370"
     CDS             54245..56380
                     /gene="igaA"
                     /locus_tag="ACG0BW_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFK7257386.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEEHSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            56445..57113
                     /gene="yrfG"
                     /locus_tag="ACG0BW_000371"
     CDS             56445..57113
                     /gene="yrfG"
                     /locus_tag="ACG0BW_000371"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFK7257387.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAREYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            57124..57525
                     /gene="hslR"
                     /locus_tag="ACG0BW_000372"
     CDS             57124..57525
                     /gene="hslR"
                     /locus_tag="ACG0BW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFK7257388.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            57550..58428
                     /gene="hslO"
                     /locus_tag="ACG0BW_000373"
     CDS             57550..58428
                     /gene="hslO"
                     /locus_tag="ACG0BW_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFK7257389.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(58491..60215)
                     /gene="yhgE"
                     /locus_tag="ACG0BW_000374"
     CDS             complement(58491..60215)
                     /gene="yhgE"
                     /locus_tag="ACG0BW_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFK7257390.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYGYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLLTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKSSL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            60594..62216
                     /gene="pckA"
                     /locus_tag="ACG0BW_000375"
     CDS             60594..62216
                     /gene="pckA"
                     /locus_tag="ACG0BW_000375"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFK7257391.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(62292..63644)
                     /gene="envZ"
                     /locus_tag="ACG0BW_000376"
     CDS             complement(62292..63644)
                     /gene="envZ"
                     /locus_tag="ACG0BW_000376"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFK7257392.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(63641..64360)
                     /gene="ompR"
                     /locus_tag="ACG0BW_000377"
     CDS             complement(63641..64360)
                     /gene="ompR"
                     /locus_tag="ACG0BW_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFK7257393.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            64588..65064
                     /gene="greB"
                     /locus_tag="ACG0BW_000378"
     CDS             64588..65064
                     /gene="greB"
                     /locus_tag="ACG0BW_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFK7257394.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            65161..67482
                     /gene="yhgF"
                     /locus_tag="ACG0BW_000379"
     CDS             65161..67482
                     /gene="yhgF"
                     /locus_tag="ACG0BW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFK7257395.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSSPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            67939..68166
                     /gene="feoA"
                     /locus_tag="ACG0BW_000380"
     CDS             67939..68166
                     /gene="feoA"
                     /locus_tag="ACG0BW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFK7257396.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            68183..70504
                     /gene="feoB"
                     /locus_tag="ACG0BW_000381"
     CDS             68183..70504
                     /gene="feoB"
                     /locus_tag="ACG0BW_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFK7257397.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVNIELLATRKSVSSCCAASTTGDCH"
     gene            70504..70740
                     /gene="feoC"
                     /locus_tag="ACG0BW_000382"
     CDS             70504..70740
                     /gene="feoC"
                     /locus_tag="ACG0BW_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFK7257398.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQALNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            70942..71835
                     /gene="rpnA"
                     /locus_tag="ACG0BW_000383"
     CDS             70942..71835
                     /gene="rpnA"
                     /locus_tag="ACG0BW_000383"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFK7257399.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVTERSPKHKESLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            71913..72026
                     /locus_tag="ACG0BW_000384"
                     /pseudo
     CDS             71913..72026
                     /locus_tag="ACG0BW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(72055..72825)
                     /gene="bioH"
                     /locus_tag="ACG0BW_000385"
     CDS             complement(72055..72825)
                     /gene="bioH"
                     /locus_tag="ACG0BW_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFK7257400.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            72863..>73035
                     /locus_tag="ACG0BW_000386"
     CDS             72863..>73035
                     /locus_tag="ACG0BW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="required for utilization of DNA as the sole source
                     of carbon and energy; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFK7257401.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKP"
BASE COUNT        17685 a        19876 c        18332 g        17142 t
ORIGIN      
        1 agataactgc gattaagcag cttctttcgc atcgcgtaca gcagccagag tacgaaccag
       61 tttgccagcc gaagcttctt tcatggttgc catcaggcgt gcaattgctt cttcgtaggt
      121 cggcagagtt gccaggcggt cgatctgaga cgccgggatc agctcacctt caaaggcagc
      181 ggctttgacc tcaaattttg cattcgcttt cgcgaactct ttgaacagac gagcagcagc
      241 gcccgggtgt tccatagagt atgcaatcag ggtcggacca acaaacgcgt ctttcaggca
      301 ctcgaacgga gtaccttcaa cagcacggcg cagcagggtg ttacgaacaa cacgcatgta
      361 tacgccagct tcgcgacctg ctttacgcag ttcagtcatt ttatctacag ttacgccacg
      421 ggaatccgca actactgcag acagcgcgcc tttggctact tcgctgactt cagcaacaat
      481 cgcttgtttg tcttgaagat ttaaagccat tagctttgct cctggatgtt tgccagagaa
      541 aatctctgga actcacttca ctcctccaaa cggagagagc gtcttaatta cggtgagcag
      601 aaacaagcca gaatataaaa gaataatctt agcgttctgt caccgtctac gcaggggatt
      661 aagtttcttg cgaaactcct gcggtcttcg acggaggcct ggataggcca ggctccaacg
      721 aacaaatctt tttagctgct aactttcgtt agcatacgtg ggggtaagat tgtagacaaa
      781 atcaccgccc acgtaaaggc attagtttac agaagcgctc aggccagcct ggtcaactgc
      841 aacacctgca cccatggtgg tggagatgct aactttcttg atgtacacgc ctttcgcctg
      901 agtcggtttt gcttttttca gcgcaaccag cagagcttcc aggttttctt tcagtttgtc
      961 agcgtcaaag tccactttac cgatggtggt gtggatgatg ccgtttttgt cgttacggta
     1021 acgaacctgg ccagctttag cgtttttaac cgcttcagca acgttcggcg ttacagtacc
     1081 cactttcggg tttggcatca ggccgcgcgg ccccagaacc tggcccagct ggccaacaac
     1141 gcgcattgcg tccggagaag caataacaac gtcaaagttc atttcgcctt tcttgatctg
     1201 gtcagccaga tcttccatac ctaccagttc agcacctgca gctttagcag cttcagcgtt
     1261 tgcaccctgg gtaaatacag ctacgcgaac ggaacggcca gtaccgtgcg gcagtacagt
     1321 tgcaccacgt acgttctggt cagatttacg agcgtcgatg ccgaggttaa cagctacgtc
     1381 cacgctttct acgaatttag cagtcgccag ctctttcagc agagcgatag cttcgttgat
     1441 gtcgtactgt ttggttgcat caactttctc gcggataaca cgcatgcgct tggtcagttt
     1501 agccatttct tagtcctcca ctaccaggcc catggaacgt gcagtacctt cgatggagcg
     1561 agtcatcgct tcaatgtcgg caccagtcat gtcggcagct ttggtctgcg cgatttcctg
     1621 cagctgagcg cgggaaattt tacccacttt gtctttgttc ggcttaccgg aaccagactt
     1681 gataccagcc gcttttttca gcagaactgc tgccggcgga gtcttggtaa cgaaagtgaa
     1741 agaacggtca gcgtaaacgg tgattactac cggaatcggc agaccttttt cgatggaatc
     1801 agtttttgcg ttgaacgctt tgcagaattc catgatgttt acgccctgct gacccagagc
     1861 cggacctact ggcggactcg ggttagccat accagctgca acctgcagct tgacataggc
     1921 ttgtactttc ttagccatta taaattcctc aagttgggta ataacgcctc tgagaggctc
     1981 cccgtgatat aaaaaatagt tttacgggca aggcccataa aaacaaaagg cgcgaaattg
     2041 tattccaatc tcgcgccttg tgcaacgatt aaatcgccgc ttttttgatc gctgggttag
     2101 gctttttcaa cctggctgaa gtccagctct actggggtcg cacgaccgaa gatagaaaca
     2161 gacactttca gacgagattt ctcgtaatcc acttcttcaa caacaccgtt gaagtcagcg
     2221 aacggaccat cattaacacg gaccatttca cccggttcaa acaacgtttt cggacgcggc
     2281 ttatcaccaa cctgctgcag gcggttcata atcgcatcga cttctttatc gctgattggc
     2341 gcaggacgat cggaagtacc gccgatgaag cccatcacac gcggtacgct gcgcaccagg
     2401 tgccagctcg cgtcgttcat caccatctga acgaggacgt agccagggaa gaatttacgt
     2461 tcgcttttgc gacgctgacc gccacggatt tcaaccactt cttcggttgg taccatgact
     2521 tcaccaaaca aatcttccat gttgtgtaat ttgatatgct cacgtagcga cgttgctacg
     2581 cggccttcaa aaccggaaaa cgcctgaacg acgtaccagc gctttttagg agcttcagac
     2641 atctcagaac ctcaggccag tgataaagga taccaggcga accagaatac catccagtcc
     2701 ccacaggatc agtgacatta ctgcggtaac cgcagccaca atcagcgtgg tgtgcaatgt
     2761 ttcctggcga gtcggccaaa tgaccttacg gacttcggta cgcgcttcac gggcaaaagc
     2821 aacggtagct ttaccttttg tcgttaacag cgcgacacca cccgctgcag caatcagaat
     2881 tactacggcc agcgcacgca gcggcagcat aatgtcgcga taaaggtagt tgccgacaat
     2941 cgccaccagg agcaatgcca ccacaacgac ccacttcatc gcttccaggc cgcgcccgct
     3001 tccttgagct tcggtattcg cactcataaa ccaacctgtc agaagtattc tacaaacatt
     3061 ttcaccccgc gatcgcgagg caaaccaaat cgaaacgcgt attagctttt cggattatac
     3121 gccctcaaca gagcctgtct cagcaatgat tatgacaaag aaaatcactg atgagccagg
     3181 ttctggtacg aaagcgtgca aaaagggcat caaatgatgc ccttttagtg cgcattgcgt
     3241 caaatgttat cggcaattag ctcagaactt ttgctacaac gcccgcgcca acggtacggc
     3301 cgccttcacg gattgcgaaa cgcagaccgt cgtccatcgc gatcgggtgg atcagggtaa
     3361 caaccatttt gatgttgtcg cccggcatta ccatctctac gccttccggc agttcgatgg
     3421 taccagtcac gtcagtagta cggaagtaga actgcggacg gtagcctttg aagaacggag
     3481 tatgacggcc gccttcatct ttggacagaa tgtacacttc agattcgaac ttggtgtgcg
     3541 gcttgatggt gcccggctta gccagtacct gaccacgttc gatttcttca cgtttgatac
     3601 cacgcagcag aacacctacg ttctcaccag cacggccttc gtccagcagt ttgcggaaca
     3661 tttcaacgcc agtacaggta gacttctgag tctctttgat accaacgatt tcaacttctt
     3721 caccaacttt gatgataccg cgttctacac gaccagtaac aacggtacca cgaccggaga
     3781 tggagaatac gtcttcgatc ggcagcagga acggcttgtc aatcgcacgc tctggttccg
     3841 gaatgtaaga atccaagaag ccagccagtt ccaggatttt cgcttcccac tctgcgtcgc
     3901 cttccagcgc tttcagagca gaaccacgaa cgatcggagt gtcgtcgccc gggaagtcgt
     3961 actgagacag aagttcacga acttccattt caaccagttc cagcagctct tcgtcatcaa
     4021 ccatgtcgca tttgttcagg aacacgatga tgtacggaac gcctacctga cgacccagca
     4081 ggatgtgctc acgagtctgc ggcatcgggc cgtcagtcgc agcaactacc aggatcgcgc
     4141 cgtccatctg agcagcaccg gtgatcatgt ttttaacata gtcggcgtgc cccgggcagt
     4201 ctacgtgtgc gtagtgacgg gtcggggtgt catattcaac gtgagaagtg ttgatggtga
     4261 taccacgagc tttttcttcc ggcgcgttat cgatctggtc gaatgcacga gcagcaccgc
     4321 cgtaggtttt agccagtacg gtggtgattg cagcggtcag agtagtttta ccgtggtcaa
     4381 cgtggccgat agtaccaacg ttaacgtgcg gttttgtacg ttcaaatttt tctttagaca
     4441 cggctatatt ccttactata gtgctctccc cttcaggaga gagcacggga ctttggtatt
     4501 aacccttagg cttatttacc acgggcttca attacggcct gagcaacgtt actcggcgct
     4561 tcatcatact tcaggaattc catggtgtat gatgcacgac ctttggtcag agaacgcagc
     4621 tgagttgcat atccgaacat ttcagacagc ggtacttcag cgtggatctt aacgccagta
     4681 acttcagatt cctgaccttt gagcatacca cgacgacggc tcaagtcacc gataacgtca
     4741 ccggtgttct cttccggagt ttctacttca accttcatga tcggctcaag cagaactggt
     4801 ttcgctttct taaagccttc tttaaaggcg atagaagcag ccagtttaaa cgccagttca
     4861 gaggagtcaa cgtcatggta agaaccgaag tgcagacgaa tacccatgtc tactaccggg
     4921 tagcctgcca gcggacctgc tttcagctgt tcctggatac ctttatcaac ggccgggatg
     4981 tattcgccag ggattacacc acctttaatg tcgttgatga actcgtagcc tttcgggttt
     5041 gaacccggct ccagcgggta catgtcgata acaacatgac catactgacc acgaccacca
     5101 gactgtttcg cgtgtttacc ttcaacatcg gtaactttct ggcggatagt ttcacggtaa
     5161 gcaacctgcg gtttacctac gttcgcttca acgttgaatt cacgcttcat acggtcaacg
     5221 atgatgtcga ggtgcagttc gcccataccc gcgatgatgg tctggttaga ttcttcgtca
     5281 gtccatacac ggaaagacgg gtcttcttta gccagacggc ccagagccag acccattttt
     5341 tcctggtcag ctttggtttt cggttcaact gcgatggaga ttaccggctc agggaattcc
     5401 atacgttcca gaatgatcgg cgcatccggg tcacacaggg tgtcaccagt ggttacgtct
     5461 ttcagaccga tagcagcagc gatgtcgccc gcgcgaactt ctttgatctc ttcacgtttg
     5521 ttagcgtgca tctgaacgat acgaccgaaa cgctcacgtg cagctttcac ggagttcagt
     5581 acggtatcac cagagttaac cacaccggag taaacacgga agaaggtcag gttaccaaca
     5641 aacgggtcgg tagcgatttt gaacgccagt gcagagaacg gctcgtcatc acttgcgtga
     5701 cgttcagccg gagtgtcttt accgtcgtcc aggataccgt tgatcgcagg tacgtcaacc
     5761 ggggatggca ggtaatcgat taccgcatcc agcatcgcct gaacaccttt gttcttgaac
     5821 gcagaaccac aggttaccag gatgatttcg ttgttcagaa cgcgctgacg cagagcacct
     5881 ttaatctctt cttcagtcag ttcttcacca cccaggtatt tttccatcag ctcttcagaa
     5941 gcttcagctg cggattcgat caggttctgg tgccattcgt tagccagttc aaccatgtct
     6001 gccgggatat cttcgtattc gaaggttacg ccctggtcag cgtcgttcca gttgatagct
     6061 ttcattttca ccaggtcaac aacaccggtg aaatgttctt cagcaccaat cgccagctgc
     6121 agcggaaccg ggttcgcgcc cagacgggtt ttgatctggt taacaacttt caggaagttc
     6181 gcacccatgc ggtccatttt gttaacgaac gcaatgcgcg gaactttata tttgtttgcc
     6241 tgacgccata cggtttcaga ctgcggctga acaccaccaa ctgcgcagta aaccattacc
     6301 gcaccatcga gaacacgcat ggaacgttct acttcgattg tgaagtcaac gtgccccggg
     6361 gtgtcgatga tgttgatgcg atgcggctca tactgcttag ccataccaga ccagaatgca
     6421 gtagtcgcag cggaagtgat ggtaatacca cgttcctgct cctgctccat ccagtccatg
     6481 gttgcagcgc cgtcatgaac ttcaccgatt ttatggttta caccggtgta gaacagaata
     6541 cgttcggtag tagtggtttt accggcgtcg atgtgcgcac tgataccgat gttacggtag
     6601 cgtgcgatgg gtgttgtacg agccatttgt ttcctcgttt atcttttagg cgttcaattt
     6661 aagtagccca aagcgggctg cttactggaa gcgcccgcct ggtgactaaa actccgaagg
     6721 gattaccaac ggtagtgtgc gaacgccttg ttggcttcgg ccatacggtg aacgtcttca
     6781 cgtttcttaa ctgcagtacc tttgttttct gcagcatcag aaagttcgtt cgccaggcgc
     6841 agagccatgg atttatcacc gcgtttacga gcagcttcaa cgatccaacg cattgccaga
     6901 gcattacgac gaaccggacg gacttcaact ggtacctgat aagtagaacc accaacgcgg
     6961 cgagacttaa cttctacagt cgggcgcacg ttttcgagag ctacttcgaa tgcttccagt
     7021 tcagatttac cagagcgctg agccagggtc tccagcgcgc tgtatacgat agattcggca
     7081 gtagattttt taccatctac catcaggata tttacaaatt tagccagcag ttctgatccg
     7141 aacttcggat ccggcagaat tttacgctga ccaatgacgc gacgacgtgg catggaaata
     7201 ctccgttgtt aattcaggat tgtccaaaac tctacgagtt tagtttgaca tttaagttaa
     7261 aacgtttggc cttacttaac ggagaaccat taagccttag gacgcttcac gccatacttg
     7321 gaacgagcct gcttacggtc tttaacgccg gagcagtcaa gcgcaccacg tacggtgtgg
     7381 taacgaacac ccgggaggtc tttaacacga ccgccacgga tcaggatcac ggagtgctcc
     7441 tgcaggttgt gaccttcacc accgatgtag gaagtcactt cgaaaccgtt agtcagacga
     7501 acacggcata ctttacgcag cgcggagttc ggttttttag gagtggtagt atatacacga
     7561 gtacatacgc cacgtttttg cgggcatgct tccagcgcag gcacgttgct tttcgcaact
     7621 ttgcgagcac gtggtttgcg taccagctgg ttaactgttg ccattaaata gctcctggtt
     7681 ttagcttttg cttcgtaaac acgtaataaa acgtcctcac acaatatgag gacgccgaat
     7741 tttagggcga tgccgaaaag gtgtcaagaa atatacaacg atcccgccat caccaggcca
     7801 tctggctgga gtgcttaacc gtaagtctga cgaaatcagt atagtcaatg ggaatgatgt
     7861 cgttcgaaat ttgaccagtc aaaccgcggg caataaggtc ttcgttcagg gcatagacct
     7921 taatgggggc attacgcaga ctttcaaggt agcggttacc gtcaactgcg gcagttacac
     7981 catcttgcaa taatagcagt tcgtcgcctt cactgagtag acgcaacagc gcagcaaaat
     8041 ccgtcagcca gggtgaacga tgtaatgtgt gcagcataaa cgcctcaaaa cctcaaaata
     8101 acatcgtagt tggcgagttc gcggcgtaag gcatctgctt cgagcggcgt ggcttcgaca
     8161 acaaagggtg tctgcggatc taacccgcgt tcgcgcagtg aagccgcaca aacccagcac
     8221 tgttcaatgt cgtacagacc caacaattta aaagtggcaa tgtaatcacg cgccagcact
     8281 gcatcgggct tttgtccttg cagcagctga aaaacgccat cagcaataaa gaagacggcc
     8341 agatcgtcag ttaatgcgga agttgccagt aaagcatcta aaccttcccg accagcagct
     8401 gtaccatgag gtgcagtaga aaaaacaaac gcaattcgtt tcatcagaac tgtaccaccc
     8461 tgtcacaggt cagcgaggct tccgccagcg cgccaagtcc gcttaaggta aatcccgact
     8521 gaagatttga cgaagccagc cccagccttc ctgcttcagt ttcatcgacg ataccgcggc
     8581 gtaacgctgc cgctacgcag atattcagcg ccacaccatg ttgcgcattc aattgttgcc
     8641 agccgcgcac aaggtcaaat tcatcgcttg ccggtgaggt caattggtta gcgttataga
     8701 ccccttcccg ataaaaaaag acgctgctta actcatggcc ttctgcaatc agcgcctgcg
     8761 caaactgaaa agcactactc gcctgttgcg taccgtatgc tggcccggtc accacgatgg
     8821 caaaacgcat tacttatctt gcccctggaa atcaccgctc ttgaactggc ggatgtagag
     8881 atagacagtg tgtttggaga tgttcaggcg gtcagcaacc tggttgatcg cgtctttaat
     8941 atcgaagatc cctttctcgt agagattcag cacgatctga cgatttttgg cgttattaga
     9001 aacattgcga tcggcattca cttcttcgat ggtgaactcc agcgtttggg taaccagatc
     9061 ttcaacagaa gaggcaaagt tgacgcttga accgacatcc ggggtttctg gcggcacaaa
     9121 agtgctcata atctgcgaga agggaacatc aagattcata ttgatgcaca gcagaccaat
     9181 tacacgctgt tcgcggttac gaatcgcgat agtcagggac ttcattaata cgccgctttt
     9241 ggcgcgagta aagtagcatt tagaaacgct gctatccgct cccgtcatat cgtgcagcat
     9301 acgtagcgcc aggtcggtaa ttggcgaacc aatcttccgg cctgtatgtt caccgttagc
     9361 aatgcgaatg gctgaacatt ttagatcctg caaagagtgc aaaacgattt cacagtggga
     9421 gccaataagc atcgctaacc cgtccaccac cgcttcgtag gatttcagaa tatcaaaatc
     9481 ggtctggtcg aaaggacgtt gatccagtaa atccaactca ctggtttcgt tggttaaaag
     9541 cgacctggac atgaaaaaaa acactccttt tcaggagcct gtcgttaact tttcagggca
     9601 ggctcattaa tgatgcgggt aactaaatta atacagcgga ggttccgctt tccagcacta
     9661 attatatccg gcctgtaata aaaaaaccgc cgcctggtca ggcggcggtt cttaatgctt
     9721 attttttagc agaatctgcg gctttcgcat cagcttccgg ctttgcatca gccttcggcg
     9781 ctggtttcac atccagcagc tctacgtcaa acaccagggt agaattcggt gggatccccg
     9841 gaacacccgc tttgccgtaa gccagttctg gtggaataac cagtttgatc ttaccgcctt
     9901 tcttgatgtt cttcagacct tctgtccaac ccggaataac accgtccaga cggaaagaga
     9961 gcggttcacc acgggtgtaa gagttgtcga actctttacc gtcgatcagc gtacctttgt
    10021 agttcacgac aacagtatcg ctgtctttcg gtgcttcgcc tttaccggct tcgactacct
    10081 gataaaccag accagttgaa gaggttttca cacctttctc tttggcaaat ttctcgcggt
    10141 actctttacc ttttgcttcg ttatcagccg cgtctttttc catcttcgcc tgagcagaag
    10201 acttcacgcg agcttcgaat gcttgcagtg tctgttcgat ctcttggtca gagagtttgc
    10261 tcttatcagc aaatgcatcc tgaacaccag cgatcagctg atctttatcc agtttgatgc
    10321 ccagtttttc ttgttcttta agagagtttt ccatgtaacg acccagcgaa gcacccagtg
    10381 cataagctga tttctgatcg tcatttttga acgctgcttt gctgtcagca gttgtagcag
    10441 gttttgcagc ttcagcagca aaagtgatcg gtgcatgcag ggcaacggcc attgtggtcg
    10501 ccagcagcgt tactttaaac agtgatttca tccatatctc caggatcggg gcatctcacc
    10561 ccagggttaa ctattatcag aagggtacta taaagcgttg tcgaacaaat ctacatacag
    10621 acacgcccta ttatcatcta ttttcagact ctttttgttt aaattagttt cgatgaccgc
    10681 gaaatgagtg ctgtctcggg cagcaaagtt aagtagaatc cgcggcgacc attcgacaaa
    10741 agaggtgaat catgcaggat ttatcatttg aagcacgcct ggcagagctg gagagccgac
    10801 tggcttttca ggaaatcacc attgaagaac tgaacgtcac ggtgaccgct catgaaatgg
    10861 agatggcgaa actgcgcgat catctgcgtc tgctgaccga gaagctaaaa gccagccagc
    10921 cgtcgaacat cgcgtcgcag gctgaagaaa cgccaccgcc acattattga ggcgtaaaaa
    10981 aagcggggat tccccgcttt tttgtcactt tttcggtatt agtggcaacc gcaaccgccg
    11041 ttacctttac cgccgcagca gccttcgcca ccgtgttcat gaccgtgatc gtggccatga
    11101 ccaccgcagc aaccgtcgtg gtcgtgatcg tggtgatgat cgtgcgcacc gtgaacgtgg
    11161 ccatgagcca gttcttcttc agtcgcttcg cgaatcgcca caacttcaac gttgaatttc
    11221 aggttctgac cggccagcat gtggttacca tcaaccacga cgtgatcgtc ttcaaccgca
    11281 gtgatttcaa ccggtaccgg accctggtca gtttcagcca ggaaacgcat acctacctgc
    11341 agttcatcaa cgcccataaa tacgtcttta ggaacgcgtt gcaccaggtt ttcgtcgtac
    11401 tgaccgtaag cgtcgttcgc gccaacagcg acatcaaatt tgtcgccaac ttcgtgacct
    11461 tccagcgccg tttccaggcc agagatcagg gaaccgtgac catgcaggta gtccagcggc
    11521 gcactcaccg gagactcatc aaccaacaca ccgtcttctg tacgtacctg ataggccagg
    11581 ctgaccacca ggtcttttgc tactttcatg atatctcctg agcatgggaa gaatagtggc
    11641 gcagattgta gcggaattct gcagccgtgt actcactagc ttaaaaaaac tcggcgcata
    11701 tcgctagtcc ggatgaaaaa tcccgatcac ttgctcatct ttgcgaacgt gatcgcgggc
    11761 ttctttgtct gcttctcgca tctgatgtcc gcacttaaca cattcaacaa tatcaatatt
    11821 attttcgcgc cacatcgcca ttgaatcctg cgcctgacag gccgggcatt ttgcgctcgc
    11881 aataaaacgt tttcggattg ccatctttac cctctactca aattcatccc agccgtccag
    11941 ctggcgtcgt tcttgttgca tttcacgctg gaaaatctct tccagttcgc gtcgggcttc
    12001 cctggcgcga gaaatttgta cggtatcggc atgcattggg atgagctctc gcaacatccg
    12061 catatccagg cgacgaaaat gcagctgcgc ccgctgtgcc tgatgcggat gcatgccaag
    12121 cgtgaccagc gtcttgcgtc ccagctctaa cgcactggag aatgtctcac gagaaaactg
    12181 cgtcacccct gcctgtaata actcatgcgc ctccacacgt ccgcgcgctc gcgcaagaat
    12241 atgtaaatgc ggaaagtgct gctggcatat ttccaccagt ttcatggtgt cttccggctc
    12301 gttacaggta atgacgatag actcagcggc ctctgcaccc gcagaacgta acagatcgac
    12361 ctgcgtggcg tcgccgtaat aaactttgta gccatattta cgcatcaggt taacggcact
    12421 gatatcccgc tccagcacgg taatgcgcat tttatttgcc atcagcaagc gaccaatcac
    12481 ctgaccaaaa cgcccaaagc ccacgacaat gacctggggt ttatcatcgt tgacccacgg
    12541 tttttcgtct tcttcttccg gtccgttaaa ctggcgggat agccatttat ccaccagctt
    12601 catcagcaac ggtgtggtca tcatggaaag cgtcaccgtc accagcaaca gcgccatctg
    12661 gtcgccctgg aataagcgtt gcgaagaagc ggtagaaaag aggacaaagg caaactcgcc
    12721 gccctgactc aacacgccag caaactgcat ccgctctgag ctacgcacgc catacaatcg
    12781 cgccagcaga tacagcacga gaattttcac cgccaccagc acaaccacag ctatcactac
    12841 ccacaacaga tgggtataaa gcaccccgag gttgagtgac atgccgacag agataaagaa
    12901 caaaccgagc agcaagcctt tgaagggatc gatagccgtt tccagttcat ggcgatattc
    12961 actttccgcc agcagcacgc ccgcaataaa cgtaccgagc gccatcgaca gccccagcgc
    13021 atccataaat aacgcggaac ccaacaccag cagcagagtc gcggcggtga acacttcccg
    13081 cacgccagaa gctgcaataa aacggaatac cggacgcagt aaataacgcc cgccaatcag
    13141 catgccgaca aacgccagca ccttcatgcc gatcttcatc cagtcgaaat gttcgtctgc
    13201 cgaccccgcc aacaacggca ctaacgccag tgccgggatc accgccagat cctgaaacag
    13261 cagtaccgag aaacctaact ggccggattc gctgcgattc atccctttat cacgcatcag
    13321 ttgcaacgcc attgcagttg aagacatcgc aaggccaatg ccaccgacca ccgccgcctg
    13381 ccaggcgaaa tccgtcagca tcaataatcc cgccagcaac gccgcgctta acagcacctg
    13441 tgccgcacct acgccaaaaa tcgaacgccg caattgccaa agcttggagg gattcaactc
    13501 caggccgatg ataaacatca ggaacaccac gccgagttcc gaaaagtgga ggatctcatc
    13561 gacgtcgcta ataaaaccca gcccccacgg gccaattgca atccctgcca gcaaatatcc
    13621 caacacagcg ccaataccca gccgcgatgc cagcggcacc gcagccaccg ccgcgaagag
    13681 aaacaacact cctgcgagta aaaaatcgga accttccatc agcggcctcc tggagacagc
    13741 ggatttgcca gccagtcacc gtaggctctg gcgtgactcg ccagctcctg tgcgctttgc
    13801 cgtctcgccc agtaaataat gatgggactc aaccaatgca tccggcacat gcccgccgcc
    13861 agttcaaagg ggcgcagaac atcgctcatc gggtagcgat tcaaggcgtc ataacggtaa
    13921 gcactttccg gctcgccggt ggtaatcacg ctacgccagt actttcccgc cagttggttt
    13981 cctcccggcc cgctggcaaa accacggctt aatacccggt ccagccactc tttcagtagc
    14041 gccgggcagc tataggtata aagaggatgc tgaaagacaa tcacctcgtg ctcgcgcagt
    14101 aatgcctgct cacgggggat atcaataaaa aaatcgggat agtgcgcgta aaggtcgtgc
    14161 acggtaacat tgctgagctg cgtggccggt ttaagcagta cccggtttgc caccgagtcc
    14221 tgagattccg gatgggcata cagcagcaaa actttcgctg gctgagacat catccccctc
    14281 ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc attgcgcccg gtgcggcagc
    14341 tcgcccatac attacattat cataatgata agttaacata gtctgaacat acggcacctt
    14401 atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc gcgtcctgct ggataatgcc
    14461 accgccacca tcaaccccgg gcagaaagtc ggcctggtgg gtaaaaacgg ctgtggtaaa
    14521 tctaccctgc tggcattgct gaaaaatgaa ctcagcgccg acggcggcag ctacaccttt
    14581 ccgggaagct ggcaactggc gtgggtgaat caggaaacgc cggcgttacc gcaagcagcg
    14641 ctggaatatg tcattgacgg cgaccgtgaa tatcgccaac tggaagcgca gctacgcgac
    14701 gccaacgaac gtaacgacgg gcacgccatt gcgaccattc atggcaagct ggatgctatt
    14761 gacgcatgga gtattcgctc ccgcgccgca agtctgttgc acggcctcgg tttcagcaat
    14821 gaacaactcg agcgtccggt aagtgatttc tccggtggct ggcgtatgcg tcttaacctt
    14881 gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg acgaaccgac taaccacctc
    14941 gatctcgatg ccgttatctg gctggaaaaa tggctgaaga gctatcaggg cacgctgatc
    15001 ctgatctctc acgaccgcga tttcctcgat ccgatcgtcg ataaaattat tcatatcgaa
    15061 caacaaagca tgttcgagta caccggcaac tacagttcgt ttgaagttca gcgcgccacc
    15121 cgtctggcgc agcaacaagc gatgtacgaa agccagcagg aacgcgtggc gcatctgcaa
    15181 agttatatcg accgtttccg tgccaaagcc accaaagcga agcaggccca gagccgcatt
    15241 aagatgcttg agcgtatgga gctgattgcc cccgcgcacg tcgacaaccc gttccgcttt
    15301 agcttccgcg cgccggaaag cctgccaaat ccgttactga agatggaaaa ggtcagcgca
    15361 ggctatggtg atcgcattat tctcgactcg attaaactga atctggtccc cggctcgcgc
    15421 attggtctgc taggccgcaa cggcgcgggt aaatcgacat taatcaaact gttagccggt
    15481 gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag ggatcaagct cggctacttc
    15541 gcccagcatc aacttgaata cctgcgcgcc gacgaatcgc ctattcaaca tctggcacgt
    15601 ttagcgccgc aggaactgga gcaaaaactg cgtgactacc tcggcggctt tggtttccag
    15661 ggcgataaag tgacggaaga aacgcgccgc ttctccggtg gggaaaaagc ccgcctggtg
    15721 ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc tcgacgaacc gactaaccac
    15781 cttgacctcg acatgcgtca ggcactgact gaagcattaa tcgagttcga aggcgcgctg
    15841 gttgtcgtct cgcacgaccg tcatttgctg cgttccacca ctgacgatct ctacctggtt
    15901 cacgatcgta aagtcgaacc gttcgacggc gatctggaag attatcaaca gtggttgagc
    15961 gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa aagagaacgc aaacagcgcc
    16021 caggcacgta aagatcagaa gcgtcgggaa gcggagctgc gtgcgcaaac ccaaccgctg
    16081 cgtaaagaga tcgcccgtct ggaaaaagag atggaaaagc tgaacgcaca gctggcgcag
    16141 gcggaagaga aactcggcga cagcgaactg tacgatcaaa gccgtaaagc ggagttgacc
    16201 gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg aagagtgcga aatggcgtgg
    16261 ctggaagccc aggagcagct tgagcagatg ctgctggaag gccaaagcaa ctgatggcgc
    16321 agataacgac gaccgatgcc aatgaattca gcagcagtgc tgaattcacc cccatgcgcg
    16381 gctttagcaa ttgtcatcta caaaccatgc tgccgcgtct gtttcgtcgt caggtgaaat
    16441 tcaccccaca ctggcagcga ctggagttgc ccgacggcga ttttgtcgat ctcgcgtgga
    16501 gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt ggtgtttcac gggctggaag
    16561 gcagtctcaa tagcccttac gcccacggtc tggttgaggc ggcacaaaag cgcggctggc
    16621 tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc aaaccgtatg caccgcattt
    16681 accattcggg cgaaaccgaa gacgccagct ggtttttacg ctggctgcaa cgcgaatttg
    16741 gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg taatatgctg gcctgtttgc
    16801 tggcaaaaga aggtaataac ctcccgattg atgccgcggt aattgtctcc gcgccattta
    16861 tgctggaggc ctgtagttac catatggaaa agggcttttc ccgcgtttat cagcgttact
    16921 tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc agcctacccc ggaacactgc
    16981 cgattaatct cgcgcagtta aaatcggtac gtcgcatccg tgaatttgac gatctcatca
    17041 ccgccagaat tcacggctac gctgacgcta tcgactatta tcgtcagtgt agcgccatgc
    17101 cgatgctcaa tcggatagcc aaaccgacgc tgattattca cgccaaagac gatccgttta
    17161 tggatcatca ggtgatcccg aaaccggaaa gtctcccccc gcaggtggag tatcagctga
    17221 ctgaacatgg cggtcatgtt ggctttattg gcggtacatt acttcatccg caaatgtggc
    17281 tggagtcacg tattcctgac tggttaacaa cgtatctgga ggcgaaatca tgttgattcc
    17341 gtggcaagac ctctcccccg aaacgctgga aaatttaatt gaaagctttg tgttacgtga
    17401 aggcaccgat tatggtgaac atgagcgtac acttgaacag aaagtcgccg acgtcaaacg
    17461 ccagctacag tgcggagaag cggtgctggt atggtcggag ctgcacgaaa cggtcaatat
    17521 catgccgcgc agtcagtttc gcgaataacc tgcaaccgtc ggcggacgag ataataatga
    17581 tagctaagtc atggagttac tatgtctgcc aaacatccgg tcattgcggt aacaggatcc
    17641 agcggcgcgg ggaccaccac caccagcctc gcgtttcgta aaatattcgc gcagttaaat
    17701 ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt acacccgccc ggaaatggac
    17761 atggcgatcc gcaaagcgcg cgacgccggg cggcatatca gctacttcgg ccccgaagcg
    17821 aacgactttg gcctgctgga acaaaccttc attgaatatg gtcagagcgg caaagggaaa
    17881 tctcgcaaat atctgcatac ctacgacgaa gccgtaccgt ggaatcaggt accggggaca
    17941 ttcaccccat ggcaaccttt accggaaccc actgatgtac tgttttatga aggtttacac
    18001 ggcggcgtcg tcacgccaca gcataacgtt gcgcagcatg tggacttact ggtcggcgtg
    18061 gtgcctatcg ttaaccttga gtggattcaa aaactgatcc gcgacaccag cgagcgcggg
    18121 cactcacgag aagccgtgat ggactcggta gtgcgctcaa tggaagacta tatcaactac
    18181 atcacaccgc agttttcccg cacccatctt aacttccagc gcgttcccac cgtcgacact
    18241 tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg aaagctttgt ggtgatccat
    18301 tttcgtaatc tggaagggat cgatttcccc tggctgctgg cgatgttgca gggctcattc
    18361 atttcccaca tcaatacttt agtggtgcct ggcggcaaaa tgggtctggc aatggaatta
    18421 attatgctgc cgctggtgca acgattgatg gaaggaaaga aaatcgagta actttgctat
    18481 tacgccggat aaaatactat ccggcttcac aatgggatag ttaaatcacg cggcaaccac
    18541 ttcatacgag tgagtaatat tcaccgcttt ttccagcatc aacgctactg aacaatattt
    18601 ctcggcagag agatctaccg cacgcgcaac cgctgcgtct ttcaggtcgc gaccggtgac
    18661 gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc tcttcgcggc gttcagaggt
    18721 caattttact tcacaatcga ccacatcctg acgccctttt tgcagaatag aaaccacatc
    18781 gatggcactg caaccccccg ccgccatcaa caccatttcc atcggacttg gtgctttatc
    18841 gcctgagttg ccgtccatta aaatctggtg accagaggcg gattcgccca gaaaagttaa
    18901 cccttcgacc cacttcactc gcgcttgcat ttttgctact ccactgcgtc aattttcctg
    18961 acagagtacg cgtactaacc aaatcgcgca acggaaggcg acctgggtca tgctgaagcg
    19021 agacaccagg agacacaaag cgaaagctat gctaaaacag tcaggatgct acagtaatac
    19081 attgatgtac tgcatgtatg caaaggacgt cacattaccg tgcagtacag ttgatagccc
    19141 cttcccaggt agcgggaagc atatttcggc aatccagaga cagcggcgtt atctggctct
    19201 ggagaaagct tataacagag gataaccgcg catggtgctt ggcaaaccgc aaacagaccc
    19261 gactctcgaa tggttcttgt ctcattgcca cattcataag tacccatcca agagcacgct
    19321 tattcaccag ggtgaaaaag cggaaacgct gtactacatc gttaaaggct ctgtggcagt
    19381 gctgatcaaa gacgaggagg gtaaagaaat gatcctctcc tatctgaatc agggtgattt
    19441 tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt agcgcatggg tacgtgcgaa
    19501 aaccgcctgt gaagtggctg aaatttcgta caaaaaattt cgccaattga ttcaggtaaa
    19561 cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt cgtctgcaag tcacttcaga
    19621 gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc attgcacaga ctctgctgaa
    19681 tctggcaaaa caaccagatg ctatgactca cccggacggt atgcaaatca aaattacccg
    19741 tcaggaaatc ggtcagattg tcggctgttc tcgtgaaact gtgggacgca ttctgaagat
    19801 gctggaagat cagaacctga tctccgcaca cggtaaaacc atcgtcgttt acggcactcg
    19861 ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca ttttgtttcc cccgatgtgg
    19921 cgcagactga tttatcaccc cgatatcaac tatgcactgc gacaaacgct ggtgctatgt
    19981 ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat tcggtctgct cttctccctc
    20041 gttcctgcct gttgcaatat tgcgggcctt gatacgcctc ataaacgttt tttcaaacgc
    20101 ttaatcattg gtgcgtcgct gtttgccacc tgtagcttgc tgacacagct actactggca
    20161 aaagatgttc ccctgccctt tttgctgacc ggattaacgc tggtacttgg cgtcactgct
    20221 gagctggggc cattgcacgc aaaattgctt cccgcatcgc tgctcgccgc catttttacc
    20281 ctcagtctgg cgggatacat gccggtctgg gaaccgttgc tcatctatgc gttgggcact
    20341 ctctggtacg gattgtttaa ctggttttgg ttctggatct ggcgcgaaca accgctgcgc
    20401 gagtcactaa gtctgctgta ccgtgaactg gcagattatt gtgaagccaa atacagcctg
    20461 cttacccagc acaccgaccc tgaaaaagcg ctgccgccgc tgctggtgcg ccagcaaaaa
    20521 gcggtcgatt taattaccca gtgctatcag caaatgcata tgctttccgc gcaaaataat
    20581 aacgattaca agcggatgct gcgtattttc caggaggcgc tggatttgca ggaacatatt
    20641 tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg tcgagcgtag ccatgcggag
    20701 gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc tgcgcgtgct ggctgatgac
    20761 attctttacc atcgcctgcc gacgcgtttt acgatggaaa agcaaattgg cgcactggaa
    20821 aaaatcgccc gccagcatcc ggataatccg gttgggcaat tctgctattg gcacttcagc
    20881 cgcattgccc gcgtgctgcg cacccaaaaa ccgctctatg cgcgtgactt actggccgat
    20941 aaacagcggc gaatgccgtt acttccggcg ctgaaaagtt atctgtcact aaagtctccg
    21001 gcgctacgca atgccggacg actcagtgtg atgttaagcg ttgccagcct gatgggcacc
    21061 gcgctacatc tgccgaagtc gtactggatc ctgatgacgg tattgctggt gacgcaaaat
    21121 ggctatggcg caacccgtct gaggattgtg aatcgctccg tgggaaccgt ggttgggtta
    21181 atcattgcgg gcgttgccct gcactttaaa attcccgaag gttacactct gaccttgatg
    21241 ctgattacca ccctcgccag ctacctgata ttgcgcaaaa actacggctg ggcgacggtc
    21301 ggctttacca ttaccgcagt gtataccctg caactgttgt ggctgaacgg tgagcagtac
    21361 atccttccgc gtcttatcga taccattatt ggttgtttaa ttgctttcgg cggtactgtc
    21421 tggctgtggc cgcagtggca gagcgggtta ttgcgtaaga acgcccatga cgctttagaa
    21481 gcctatcagg acgcgattcg cttgattctg agcgaggacc cgcaacctac gccactggcc
    21541 tggcagcgaa tgcgggtaaa tcaggcgcat aacactctgt ataactcatt gaatcaggcg
    21601 atgcaggaac cggcgtttaa cagccattat ctggcagata tgaaactgtg ggtaacgcac
    21661 agccagttta ttgttgagca tattaatgcc atgaccacgc tggcgcggga acaccgggca
    21721 ttgccaccgg aactggcaca agagtattta cagtcttgtg aaatcgccat tcagcgttgt
    21781 cagcagcggc tggagtatga cgaaccgggt agttccggcg atgccaatat catggatgcg
    21841 ccggagatgc agccgcacga aggcgcggca ggtacgctgg agcagcattt acagcgggtt
    21901 ataggtcatc tgaacaccat gcacaccatt tcgtcgatgg catggcgtca gcgaccacat
    21961 catgggattt ggctgagtcg caagttgcgg gattcgaagg cgtaatacag gctaaaccat
    22021 cggataaggc attcacgccg catccgacat cttttgcctg atgcgtctta tttacgcccc
    22081 aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct tcatcgatat ccgcatcttc
    22141 caccaccagc gacggtgcaa aacgcatcac atccggtccg gcattcagca ccattacgcc
    22201 agcctctgcg cccgcataca ggaaatcacg cgtccgacct ttgtactgtg gtttcagctc
    22261 cgcgccaatt aacagcccca taccgcgaat atcgctaaac acatcgtact gctgatcgat
    22321 cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga atgccttcca gcacttcagg
    22381 ggtattgatg atatcaaacg ccgcgcccgc tactgcacag gccagaggat taccgccgta
    22441 ggtggaaccg tgagaaccag gatgaaatgc agaagcaatt tccgccgtgg tcagcatggc
    22501 gctaatcggg aagccgccgc ctaacgcttt cgcagaggtc agaatatccg gcgtcacgcc
    22561 gtagtgcatg taagcaaaca gatcgccggt ccgccccatc ccgcactgca cttcatcaaa
    22621 caccaataat gcctgatgtt gatcgcacag ctcgcgcaag ccctgcaaaa actctggcgt
    22681 cgctgccgtc acgccgccct cgccctggat cggctcaacc accaccgcac aggtgtgatc
    22741 atccatcacc gctttcactg cgtggatatc gttaaaaggc acgtggatga tgtctgccgg
    22801 ttttggccca aagccgtcgg aatattttgg ctgcccaccc accgaaacgg taaacagcga
    22861 gcgaccatga aaagcgttat ggaaggcaat aattttggtt ttgaacgggc tatgacgcac
    22921 acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta gcttccgtgc cggagttcat
    22981 aaacaccacg cgttcggcaa acgttgcctc aatcagttta cgaccaagac gcagcgccgg
    23041 ttcattggtg aaaacgttac tgatatgcca cagagtttcg ccctgggttt ttaacgcgtt
    23101 caccagcgca ggatggcaat ggcccaacgc cgtaactgca atgccacccg cgaaatcgac
    23161 atactccttg ccttgctgat cccagattcg gctcccctga ccttttaccg gaataaactc
    23221 tgccggagca taaatcggca ggatcacttc atcgaaagtc gcgcgtgtaa ttgctgtttg
    23281 ttcagttgcc atctcatgat caccctgtta cacataaata aatgtgaaat tataatcaca
    23341 aaatatgcat aaaaaatcac ttaatagcaa tcagaaatca gcgatgcagg aaattagcca
    23401 gcagttgatg tccttgttcg ctgagaatac tttctggatg gaactgcacg ccttccagat
    23461 cccactggcg atggcgaatc cccataatct cgcgggtttc gctccaggcc gtcacttcaa
    23521 agcacgctgg taatgagtca ggttccacca ccagcgaatg gtagcgtgtc acggtaagtg
    23581 gatttgccag cccccgaaat acgccctcac cgttatgtgt aatcggcgag gttttgccgt
    23641 gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc ctgcgccatt gcctgatgac
    23701 cgaggcagac gccaagaatc ggcaagcacc cggcatagtt gcgaataacg tcaagggaga
    23761 tcccggcttc atctggcgta cagggtccgg gtgagatgac aattttttgt ggtttaaggg
    23821 catcgatatc cgccagcgtc aacgcatcgt tgcgcttaac cagcacatcc gcccccagtt
    23881 cacaaaagta ctggtagagg ttccaggtaa aagaatcgta gttatcaata agcaggatca
    23941 tggcggctcc ggtacgaaag aaccgcgcta ttttactcag attccccggc ttcgcttacc
    24001 actttgctaa atatcatctg cagcgcggtg agatccccca ttgccccact ctgatttgcc
    24061 tgattccagg cctctttttc gataccctgc cagctcagtt ggtagcccgc atgaatcgcc
    24121 agctgctcga agaagatccg ctgtgccagt ccgcttccca cacggaaggg atgcagcacg
    24181 ttgatttcac aatagtaatg cgccagccgc tcgacgaact tcgctttatc caggccaacc
    24241 agataaccgt cttcctccag atcctgcatc agggcattgc cctctttttc gatataagca
    24301 aagtggcaga acggcgtatc accctgataa atatcaactt cacgcagttg ccccgcccag
    24361 tcgaaaatat cctgatacag ctggcgatgg atagtgcgca aatgcggtaa accgcgcacc
    24421 agcggaccaa gctcaatggt cgcagcacgt agcgccgtca tttcgtaagc ggcctgttcc
    24481 agacgttgtt gctggcggat gttcagccgg ttacgcatga tatcaaggcc aggataaaga
    24541 tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc ctcctcagct cttcaagtcg
    24601 cgctaaagcc tctgcggcag taagagtgac taaaggcatc tcgacgcctt caagacggcg
    24661 actggcctgg aaattacgat tacgctgaaa ctcccagaga cgggactttt gcttatcggt
    24721 gagtttcttc acttgatgcc tccctgaacg tgtccatttg ccacaagtat aagcggcaaa
    24781 tccgggttac gccgggagag cagaagcgcg ggcaagattg cccgcgcgag aaatcattac
    24841 ggcaggactt tagcggaaag gataactacc ggttttgacg gcacattctg gtacggacca
    24901 acatcatgag tcggcacctg ggaaatctta tcggcaatgt ccatgccttt caccacttta
    24961 ccaaataccg cgtaaccgaa atcacgctga ccgtggtcaa ggaaggcgtt atcggcaacg
    25021 ttgataaaga actggctggt ggcgctgtct ttgtcagcgg tacgcgccat cgcgatggtg
    25081 ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga ttggcgggtt tggttttttc
    25141 tgctgcatct gctcggtgaa accgccgccc tgaatcataa agccaggaat gacgcggtga
    25201 aaggtagtgt tgttataaaa accgctgttc acataatcga caaagttttg cacagacact
    25261 ggcgcttttt gtttatccag ttccagttcg atgttaccag ctgaggttgt caacaatacg
    25321 tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag cagaaagagc gaaaacagca
    25381 gccatcgccg ccagggtcga tttgaacatg agatttcctt aacaaacaga gcagaaaaaa
    25441 agcaagtgaa gagattctaa atagccttca agaacaaggc catcccttta cctaatttta
    25501 cgtatctgaa atatatgtaa cctgacgatc actttttatt ctgtgataaa aatcacctct
    25561 taaaatgcaa tttagcaacc gattgcaata aaacatttaa tcagatcaca aaatcaccta
    25621 aaatcgcccg ctcgcggcat agcccgctat gccattttat ttttgaacgc aggccaatca
    25681 tgactaacag caatcgcatc aagctcacat ggattagctt tctctcctac gcactgaccg
    25741 gtgcgttggt tattgtcacc gggatggtga tgggaaatat cgccgattat ttcaatctgc
    25801 ctgtttccag tatgagtaat accttcacct tcctcaacgc cggcatttta atctctatct
    25861 tcctcaacgc ctggctgatg gaaatcgtcc cgttgaaaac gcagttacgt tttggctttc
    25921 tcctgatggt gctggcggtt gccggtttga tgttcagcca cagcctggcg ctgttctcgg
    25981 cggcgatgtt cattctcggg gtggtcagcg gcatcaccat gtcgattggt acattcctga
    26041 taacacaaat gtatgaaggg cgtcagcgcg gttcccgcct gttatttacc gactccttct
    26101 tcagtatggc tgggatgatt ttcccaatga tcgccgcgtt tctactggcg cgcagcattg
    26161 agtggtactg ggtttatgcc tgcatcgggc tggtgtatgt cgctattttt attctgacct
    26221 tcggctgtga gttcccggca ctgggcaaac atgcaccaaa aacggatgct ccggtagaga
    26281 aagaaaagtg ggggatcggc gtgctgtttc tctccattgc ggcactgtgc tacatcctcg
    26341 gtcagttagg ttttatctcc tgggtgcctg agtatgccaa aggcctgggc atgagcctga
    26401 atgatgctgg tacgctggtg agtaacttct ggatgtcata catggtcggc atgtgggcgt
    26461 tcagctttat tcttcgcttc tttgatttgc aacgtatcct gaccgtactg gcgggtctgg
    26521 ccgcgattct gatgtacgtc tttaacaccg gaacaccggc acatatggcg tggtcaattc
    26581 tcgccctggg cttcttctcc agcgcgatct ataccaccat catcaccctg ggttcacagc
    26641 agaccaaagt accgtcgcca aaactggtta actttgtcct gacctgcggg accatcggta
    26701 ctatgttgac ctttgtggtt accgggccga ttgttgaaca tagcggtccg caggcggcac
    26761 tgcttacagc aaacggtctg tacgctgtcg tctttgtgat gtgcttcctg ttaggtttcg
    26821 tcagccgtca ccgtcagcat aacaccctga cctctcatta attgctcatg ccggacggca
    26881 ctatcgtcgt ccggcctttt cctctctaac cccgctacgt gcatctattt ctataaaccc
    26941 gttcaatttg tctgtttttt gcacaaacat gaaatgtcag acaattccgt gacttaagaa
    27001 aatttataca aatcagcaat atacccctta aggagtatat aaaggtgaat ttgatttaca
    27061 tcaataagcg gggttgctga atcgttaagg taggcggtaa tagaaaagaa atcgaggcaa
    27121 aaatgagcaa agtcagactc gcaattatcg gtaacggtat ggtcggccat cgctttatcg
    27181 aagatcttct tgataaatct gatgcggcca actttgatat taccgttttc tgtgaagaac
    27241 cgcgcatcgc ttatgaccgc gtacacctct cgtcttactt ctctcaccac accgccgaag
    27301 agctgtcgct ggtgcgcgaa ggcttctacg agaaacacgg catcaaagtt ctggtcggcg
    27361 aacgcgctat caccatcaac cgtcaggaga aggtgattca ctccagcgcc gggcgcaccg
    27421 ttttttatga caagctgatc atggcgaccg gttcctaccc gtggatccca ccaatcaaag
    27481 gttctgatac tcaggactgc tttgtctatc gcaccattga agacctcaac gccattgaat
    27541 cctgtgcccg tcgcagcaaa cgcggtgccg ttgttggtgg cggcctgtta ggtctggaag
    27601 ccgctggcgc gctgaaaaac ttaggtattg aaacccacgt tatcgaattt gcccctatgc
    27661 tgatggcgga acagcttgat cagatgggtg gcgagcagct gcgtcgcaaa atcgaaagta
    27721 tgggcgtgca cgttcacacc agcaaaaaca cccttgagat tgtgcaggaa ggtgtcgaag
    27781 cgcgtaaaac catgcgtttt gccgacggca gcgagctgga agtcgacttt atcgtcttct
    27841 caaccggtat ccgtccgcgc gataagctgg caacccagtg tggtctggac gttgctccgc
    27901 gtgggggtat tgtcattaat gattcctgcc agacttccga tccggatatc tacgccatcg
    27961 gtgaatgcgc aagctggaac aaccgtgtat ttggtctggt agcaccaggc tacaaaatgg
    28021 cgcaggtcgc cgttgaccat attctcggta gcgaaaatgc ctttgaaggt gctgacctta
    28081 gcgccaagct gaaactgctg ggcgtagacg taggcggtat tggtgatgcg cacggtcgca
    28141 cgcctggcgc acgtagctac gtttacctcg acgaaagcaa agagatctac aaacgcctta
    28201 ttgtcagcga agacaacaaa accctgctcg gtgcggtact ggtgggcgat accagcgact
    28261 acggcaacct gctgcaactg gtgctgaacg ctatcgagct gccggaaaac ccggattctc
    28321 tgattctgcc ggcgcactcg ggtagcggta agccgtctat cggtgttgat aaactgccgg
    28381 acagcgcgca aatctgctcc tgcttcgacg tcaccaaagg tgatctgatt gctgccatca
    28441 acaaaggctg ccacaccgtt gcggcgctga aagctgaaac caaagcgggt actggctgcg
    28501 gtggctgtat cccgctggtc actcaggtac tgaacgcgga actggcgaaa cagggcatcg
    28561 aagttaacaa caacctgtgc gaacactttg cttattcgcg tcaggaactg ttccatttaa
    28621 tccgcgttga aggcattaaa accttcgaag aactgctggc gaaacacggc aaaggctacg
    28681 gttgtgaagt ttgtaaaccg accgtcggtt cgctgctggc ctcctgctgg aacgaataca
    28741 ttctgaaacc ggaacatact ccgctgcagg attcaaacga taacttcctc gctaacatcc
    28801 agaaagacgg cacctactcg gtgatcccgc gttctccggg cggtgaaatc accccggaag
    28861 ggctgatggc agtaggtcgt atcgcgcgtg aatttaatct ctacaccaaa atcaccggct
    28921 cccagcgcct ggcgatgttt ggcgcacaga aagacgatct gccggagatc tggcgtcagc
    28981 tgattgaagc cggcttcgaa accggtcatg cctatgcgaa agcactgcgt atggcgaaaa
    29041 cctgcgtggg tagcacctgg tgccgctacg gcgttggcga cagcgtcggc ctcggcgtgg
    29101 aactggaaaa ccgctacaaa ggcatccgta cgccgcacaa aatgaagttc ggtgtctccg
    29161 gctgtacccg tgaatgttca gaagctcagg gtaaagacgt gggtattatc gccactgaaa
    29221 aaggctggaa cctgtatgtt tgcggtaacg gcggcatgaa accgcgtcat gcggatctgc
    29281 tggcagcgga tatcgatcgc gaaacgctga tcaaatatct cgaccgcttc atgatgttct
    29341 acatccgtac cgccgacaaa ctgacgcgta ccgcgccgtg gttagaaaac cttgaaggcg
    29401 gcatcgatta cctgaaagcg gtgatcattg acgacaaact ggggctgaac gcacatctgg
    29461 aagaagagat ggcgcgcctg cgtgaagcgg tagtatgtga gtggactgaa acggtcaata
    29521 caccgtccgc gcagactcgc ttcaaacact tcatcaacag cgacaagcgt gatccgaacg
    29581 tgcagatggt gccagagcgc gaacagcacc gtccggcaac gccgtatgaa cgtatcccgg
    29641 taactctggt ggaggacaac gcatgagcca gtggaaagac atctgcaaaa tcgatgacat
    29701 cctgcctgaa accggcgtct gcgcgctgtt aggtgacgag caggtagcaa ttttccgccc
    29761 gtatcacagc gaccaggtgt ttgcgatcag caacatcgac ccgttcttcg agtccagcgt
    29821 gctgtcacgc gggctgattg cggaacacca gggcgagctg tgggtcgcca gcccgctgaa
    29881 aaaacagcgt tttcgcttaa gcgacggctt gtgcatggaa gacgaacagt tttccgtcaa
    29941 gcattacgac gcgcgagtga aagacggcgt ggtgcagcta cgcggttaat gttttaacgg
    30001 gaggcgcaat acctcccctt tttgcatggt cctgtaataa ccttcgatat attgcaggac
    30061 attttttaaa ctttttgttt tattttttgt ttttattttt taaaggataa tcaaatgttt
    30121 acagacacta ttaataagtg tgcggctaac gctgcgcgca ttgcacgcct gtcggcaaat
    30181 aacccgctcg gcttctgggt cagctccgcc atggcgggcg cgtatgtggg tcttgggatc
    30241 atcctgatct tcacgctcgg taatttgctc gacccgtccg tacgtcctct ggtgatgggc
    30301 gcgacctttg gtatcgcctt aacgttggtc attattgctg gttctgaact gttcaccgga
    30361 cacaccatgt tcctcacctt tggggtgaaa gcgggcacta tcagccacgg gcaaatgtgg
    30421 gcaatcctgc cgcaaacctg gctgggtaac ctggtcggtt ccgtctttgt cgccatgctc
    30481 tatagctggg gtggcggtag cctgctgccg gtagatacca gcatcgttca ctccgtcgcg
    30541 ctggcaaaaa ccactgcacc ggcaatggta ctgttcttca aaggtgcatt gtgtaactgg
    30601 ctggtttgcc tggcaatctg gatggcgctg cgcactgaag gggcggcgaa atttatcgct
    30661 atctggtggt gtctgctggc atttatcgcg tccggctacg agcactccat cgctaacatg
    30721 acgctgttcg ccctctcctg gtttggcaac cacagcgaag cctacacgct ggcgggtatt
    30781 ggtcataacc tgctgtgggt gacgctgggt aatactttat caggtgccgt attcatggga
    30841 ttgggttatt ggtatgctac gccgaaagcg aatcgtccgg ttgcggacaa atttaatcaa
    30901 actgaaacag ctgccggtta attactaagg ggtttttacg tggatcattt gcctatattt
    30961 tgccaattac gcgatcgcga ctgtctgatt gtcggcggtg gtgatgtcgc ggaacgcaaa
    31021 gcaaggttgc tgttagacgc aggcgctcgc ttaacggtga atgcattagc gtttattcca
    31081 cagttcaccg catgggcaga tgcaggcatg ttaaccctcg tcgaagggcc atttgatgaa
    31141 agccttctcg acacctgctg gctggcaatt gctgcgacgg atgatgacgc gcttaaccag
    31201 cgcgtcagtg aagccgctga agctcatcgc atcttctgta acgtggtcga tgcgccgaaa
    31261 gccgccagct ttattatgcc gtcgattatt gaccgctcac cgctaatggt cgcggtctcc
    31321 tctggcggca cctctccggt tctggcgcgc ctgttacgcg aaaaacttga atcactgctg
    31381 ccgttgcatc tgggccaggt ggcgaaatac gccgggcaat tacgcgggcg agtgaaacaa
    31441 cagttcgcca cgatgggtga gcgtcgccgt ttctgggaaa aactgttcgt taatgaccgc
    31501 ctggcgcagt cgctggcaaa caacgatcag aaagccatta ctgaaacgac cgaacagtta
    31561 atcaacgaac cgctcgacca tcgcggtgaa gtggtgctgg ttggcgcagg tccgggcgat
    31621 gccgggctgc tgacactaaa aggactgcaa caaattcagc aggcagatgt ggtggtctac
    31681 gaccgtctgg tttctgacga tattatgaat ctggtacgcc gcgatgcgga tcgcgttttc
    31741 gtcggcaaac gcgcgggata ccactgcgta ccgcaggaag agataaacca gatcctgctg
    31801 cgggaagcgc aaaaaggcaa acgcgtggtg cggctgaaag gtggcgatcc gtttattttt
    31861 ggccgtggtg gcgaagagct ggaaacactg tgcaacgcgg gtattccgtt ctcggtggtt
    31921 ccgggtatta ccgcagcttc tggttgctct gcctattcgg gtattccgct cacgcatcgc
    31981 gattatgccc agagcgtacg cttaattacc ggacacttaa aaaccggtgg cgaactggac
    32041 tgggaaaacc tggcggcaga aaaacagacg ctggtgtttt atatggggct gaatcaggct
    32101 gcgactattc agcaaaagct gattgaacac ggtatgcctg gcgaaatgcc ggtggcaatt
    32161 gtcgaaaacg gaacggcagt cacgcagcgc gtgattgacg gtacgctcac gcagctgggc
    32221 gaactggcgc agcaaatgaa cagtccatcg ctaattatta ttggtcgggt tgttggcctg
    32281 cgcgataaat tgaactggtt ctctaaccat taatttaacc agggccagag aagtctggcc
    32341 tttttcgttt acaactctct ttatatattc agcagataaa aagatacccc cttaataaat
    32401 attgtctgct aatgggttat attcaccgtt acagcgactt acaaaattaa atcaatcgct
    32461 cactgacgct aattgaatat tcctgtatcg ttcttaatga gcagtctgct tatattcata
    32521 taatcaatga atattaatta atatataata catagggaat gtaaatgaac aaatttatta
    32581 aagttgcact ggtaggcgca gtactggcta cgttaactgc atgtactggt catattgaaa
    32641 accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa
    32701 tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg
    32761 tttaattgcc tgacgccctc ggggttaccc gagggcgtat ttatttctgc attcttttcg
    32821 aaaacatcat gcacaagatt aaatactggc aatactctca cgcaaaatcc gcagcaccgt
    32881 ctcttcaccg ctgcgatttg ggttaatacg aatcgcataa tgttcgagtt gcggattcac
    32941 ctggcgaaac gttccggaaa gtcgataaaa gagcggtgga atttcatatt tcgactctgc
    33001 accaaccggg taaggcaagg caccgcgctt ttgcgcctct tccagcactc tggcggcaat
    33061 cggctgatga aactcgacaa tcaacacctt cgactgtgca ttggcaatga cggcgctttt
    33121 cacttccgga actgcaccac cgttgagcaa ggccagcaac cgttcagata cccccgcctg
    33181 caccgcgtgc atcactggcg caaacaccag cccacgcaag acttccaacg cctgagcgcc
    33241 ctgaatctgg ctgccgccgg agtagagcgt ggcgcgaata cggttaataa catcagcatc
    33301 gcctaccacc gcgccaacac cctccggccc aaacagcttg aagcaggaaa aagtcgagac
    33361 atttgccccg cattcgcagc caattcgcgc taccttcatc accgcatagt tgtcatcggt
    33421 taatgcagga acgccagccg agcgcaacgt tgccagcaca tctgccagaa tgtacccgtc
    33481 ctgcggctgc tggcgcgtat gctgcaccag cgcggcatct ggttgttgct cgtcgacaac
    33541 ctgcttcagt gccaacaggt cattgaaatc agcagtgata agcgtcagcc ccatctgctc
    33601 aataatgacc tgagtcgtcg ggtaaaccgg cgcgtcatgc accagaagac gctgccccgg
    33661 tttgagcaaa gccgccaacg ccgcacggat cgcgccagta cccgcgccct gtaccagcgc
    33721 cgcagcctgt gcgtgaaatg catcagccag cacctgttcc actcgctggg taatgcgcgg
    33781 ttgattcagc cccggcgtta agcccaaatc cccgcgggta agaaactcgc tgccgggaaa
    33841 atggcggcaa atcgtgtcca ccagcgcaaa ctgcttttgc tgcgcctcaa taagcgtcag
    33901 gctttgcaaa ggatacgtct tcataggtgc cttccttaag ccggaacgaa caggccaaac
    33961 cagtaaagaa cattcagcag aataccggtg atcattactg ccacgaccgg tgccgccatt
    34021 ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaactgc gatagaaaat
    34081 ccggtataac ccgccatttt aattgctgcg aaaatcgaac cgaccagcaa cgccacttcc
    34141 atcagcatat tcatggcgtt acggatgtta tctgacgcat tacgcaccga cggatagcgc
    34201 cccagccatt tgccgatcga acgaagcagc aggacttccg ccgaaataac cactgcgcct
    34261 aataccgctg caaccatcag attcggcgag agatagccca ccgcataaac aaaggtaaag
    34321 cccgcaactg catacacacc agtggctaac gcggtggtgg caatcaacgg tacaaaaccc
    34381 agcccgcgca taaattccgc cagagctgcc tggttaatca gcgtttgtga ttgttccggc
    34441 gttacgcctg cggaatatgc tttctccagt gtgaagatcg acacttcact gccagcaaaa
    34501 atcttcatgc tggcaacggc ggcaatcagc gccccgacaa tagcgatata cggtaagttt
    34561 ttgataatcc gcgacgtgcg ttcttcaaac accgaaagcc cgctggcatc aatatcattt
    34621 tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gcagcatcac catgccaata
    34681 aagatttcga tggattccgg attgagatgc gggaaatagc gcactaccac cacacgggtc
    34741 atcagtacca ccacggcggc gatcagactt tgcttccagc caaactggta gaaaatcgcc
    34801 accaacggga acagtgcaaa agctgaaacc accggtgagc ttaattcccc caggctacct
    34861 aatacatcca ccggtagcgc ggtcagcagc tggtttaccg gcaacaggca agtaaggatc
    34921 aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc cagtacgccg
    34981 aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt cagggtaaac
    35041 gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact ccccgcctcg
    35101 cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatacc gtcatgaaaa
    35161 acggccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc caccacgata
    35221 atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc agcatcggaa
    35281 tcgcgtgttc gacatgctca acggaaagcc caaacgccac tttcccttcg gcgatcattt
    35341 tggcgatatg ttcgtctttc gctttaatgc ctggtttggc aatggtgcaa cttttgttat
    35401 agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta cacgcgccga
    35461 tgtagtaatc cagttgcccg gatttcactt tcattgccgc ttccatgtcg ttgtgaatga
    35521 aaacttcaaa acagccagga gccgtcgctt caatggtttt tttaatctgc tcacgctgta
    35581 agcctgcaac accaatcttt ttcatgagtc tgtccttatt ggaaaaattg agagggagtt
    35641 tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa ttgcggaata
    35701 aaggtggtca ataaatagtc atagccataa ccaccgttgg cttttaaatg ggagcggcgc
    35761 gtaatatcca tcgacagcat gacgcggttc agcaaaccac ggtcacgtag tgcgtggagc
    35821 atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttgc cgatggtgtc gaactgcaca
    35881 aacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tgtctttcag atcgcagtga
    35941 ccaacggtga cgcgcgaaag atcaactccg tgggcttgca gtagcgccag ttgttccagc
    36001 cccatggtgc taaaagaggt atgcgtggag atcggacgtc cggtctggtt atgtgccagc
    36061 gcggcggcaa taaacacctt ctcttccagc ggcgtaatct tcccttcgct ggagccgatc
    36121 tccgcgatga tcccggcttt cagctccgtg ccatcgatac cctgttcaat ttcatcgacc
    36181 atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa aaacgcgtcc
    36241 tggtaataac cggtgcaggc caccacgttg atccccgtcg cgtgcattac atcgagcata
    36301 aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg cacgccccgg
    36361 gccatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg gcagtccacg
    36421 ttgtttttaa agccggagag atcaatatgc agatgctcat gggcgagggt gtagcccgtc
    36481 ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa gagcgaccat
    36541 tttgtggcgg tggcgcacgg aaaaattcac atacggtagc ccccacatca gaaagcgtgg
    36601 tgcgcacacc aagctgcgta gcgaccatcc cttgctgata aaccagcact ggcaccactt
    36661 cgcgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc atcacgacca
    36721 ggcaatcatc tggctgcatc gcctcaataa gccgggcaag gttacggtca acgacctgca
    36781 aacgttccgc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt tcctggatat
    36841 tggtacaaat aaacgccgtc ggatgggtgt taaattcgtt gagggtgata tccataatcc
    36901 gctggctatc caccaggttt tgccagctca cgccataagg attactggca atatctgcca
    36961 ctttccccac cagcacggtt ggtacgccca cttcatacag tttttgcggc acctgcactt
    37021 tttcatcaac accgtagccc atatgcacga cctggaagcc gttgtcataa gcgcctgaac
    37081 gcggcgcatt gatgccaata aagcgctctt ctttactttc tgcggcatcg agaatgcgtt
    37141 gactgtcgag taacaggccg ccaaatgtaa tgacccgacc gacctgtacc tgctcacgca
    37201 caatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg gcggtgatgt
    37261 tatagacctg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc tgattgaccc
    37321 acagaaattg cagatcatcg ccaccgcgct ccacctgcca gcctgcggaa actaatgcct
    37381 gctcaacacg gtcaatcaca tcgcgaaagg gcatccgcag cggcggtaac gggcgcgtac
    37441 ctaaaatctc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc tctgccacac
    37501 cccaggttgc ggagtctgac ggctgcatat cgccaggcgc ataacccaat gcgttgatta
    37561 gccccagctt ctccagcgct ggtagctgca aatgcggcaa ctggctcagg atgtgaccac
    37621 aggtattcgc cccggcatcc tgcggacgca ccaacgtgac atctttcatt gcccctacgc
    37681 caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag agtttccaag
    37741 actgtcataa cgaccgacga tttctggttc gccacggtga ataccggaca gcaacaccac
    37801 atcgctacgg gtgacaaaaa tctgcgtgcg aaaacagagc actactgcac tgcttaccgg
    37861 aaactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag ttttgagatt
    37921 ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg catgaccacg
    37981 acggtagtaa ccgccgccgt agcagtagct gtcgccacgg aaatgatggg agatttcact
    38041 taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgcag gaatagtgcc
    38101 cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tccgccagca atggcagcga
    38161 agtgcagctg gtcgctgaag gcgcgttcag ttgctcaatc gcaataccag atttcgccag
    38221 ttgatcccgt gcttgtacca gcgtgtgaag attcggtgtc ggcaaagctt tcccggcagc
    38281 ctcatcccaa agcaggcaag ggaaatgggt taatccggct aaatgcagcc ccggcagttt
    38341 cttaatttca gcaaccacct cgtgcagcga atgctgtaca aaaccgctct cctgccccgg
    38401 ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca gactgcacac gtccggcctt
    38461 caccgctgcc gcagaaactt cccacgcttt gtcgagagta aacacggtga taacgtcggt
    38521 gccctgttca acggcgtcag caacctgatg acaagggatt tgtaccagat gcccctgatg
    38581 cgccaccggc aaaccggcgc ggcgcatgac tcgcgcctct ttgtaatcca ccaccacaat
    38641 gccgctgtag cccaatgcca gcagtttttc cgccagccac ggattacgac cgaattgttt
    38701 ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc tcaatcagcc gcttaccgtt
    38761 ttccagtatc tgatccacgt cgatcaccca gctgtccggg gcgatcttgc ccttttgcca
    38821 caagcttagt gcggcggaaa ttagcgccgg gttctggcgt ttcaatgctt ctacaaacat
    38881 cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttttgaata tttaggttgt
    38941 gcgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca taatccataa
    39001 aggttggcca gcaaatagcc ttcttcacac ggattaactt ccagtgtaaa ttccttcaac
    39061 aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag ttctgccagc
    39121 aattcggcgt ccagcggctc tatttcttca ccacgacgac tgcgcatcag tgcgctcgcc
    39181 atatgtgtca tcgccatcat tccttgctcg ctgcgcaccg gtagatgaca ctctgtttcc
    39241 agtaagttga cgacctgcat catgcctttg cagatgtcct tatcaataac gcctgcatcg
    39301 caaagcaggt tgagtctggt ttccatttca gatgcctttc gtttaataac tgggttcctg
    39361 ttccccactg accactcgtt gttgatgggc aagcagggcc tgtttatcaa caactgcacg
    39421 cagcaattgt tgcatcgggt aatcatcgac tcgcgtcaaa accacggctt cggtggcctg
    39481 taaaaatcgc ggatcgtctg tgagcggcgt cgcctctaat cccagcatgg tcagttcgtt
    39541 ttccgccacc acgttccaga tcaccgcatc gacatcgcct ttaacaatgc gttgtaaact
    39601 ctcgtgataa gagagatcga cacgttccac atcactatcg ccaaaaaaga catcggtcat
    39661 gattttctga tccgccgaac ggttatccag ccctacgcgc ttcacgtttg cggactcgcc
    39721 tttacggcaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact ccagcgcgag
    39781 gcataagcct ttttgcgtga gataactttc cgccgccagt cgggaaacaa ccgccatgtc
    39841 atacacgccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatat gcgcatagta
    39901 aaaaggaata ccatcaaact gagctttcaa tccgctcgcc aggccttcgt acaaacgggt
    39961 atagggcaag ggcatcgcgc acaccacatt gttgatatcc acatgactca gcaatgcttt
    40021 gttatccatt tccaccagat aactgccatt acgcccacgg cgttctatcc gtatcgctcc
    40081 gcttgattcc agcgttttca acgcggcctg cgtcaggcca acggatgaac ggcattcagt
    40141 tgccagttca tctatggttt tcagtcgatt accgcacttt tcacccaaca aataacgggc
    40201 cagtgtcgtg atgacgacgc cttctttttt gataaacgtt cgacgcatag taaattttca
    40261 ataaagtgaa tatttatatc ttcataaaaa tgaagataag tggcaactgc gagatgtgga
    40321 aacggcgaga cgcttcacat tttttcggat tattgcaaat gaggataaaa aaaccgggtt
    40381 tccccggttt ttggcattaa tgttaaatta cggcttcgcc acaaaaccaa tcgcttcgta
    40441 caccgctttt agcgtacggg aagcgtgtgc gctggctttt tccgcgccat ctttcatcac
    40501 ctgttgcagg aaggcttcgt cattgcggaa acggtgatag cgttcctgca attcagtcag
    40561 cataccggaa acggcatcag ccacttcgcc tttcagatga ccatacatct tgccttcgaa
    40621 ctgtttttcc agttccggga tgctctggcc cgttaccgca gaaaggatat ccagcaggtt
    40681 ggaaacgccc gctttattct gcacatcgta gcgaactacc ggcggctcgt cggagtcagt
    40741 gaccgcgcgt ttgattttct tcactaccga tttcggatct tccagcaggc cgataacgtt
    40801 attgcgatta tcgtcagact tggacatctt cttggtcggc tccagcagcg acattacgcg
    40861 cgcgccagat ttcggaataa acggctccgg caccttaaag atctcgccat acagcgcgtt
    40921 gaaacgctgg gcaatatcgc ggctcagttc gaggtgctgt ttctggtctt cacccaccgg
    40981 taccagatta gtttgataca gcaggatgtc cgcagccatc agcaccggat agtcaaacag
    41041 accagcgttg atgttctcgg cataacgcgc agatttatct ttaaactgcg tcatgcggct
    41101 cagttcgccg aagtaggtat agcagttcag tgcccagcct aactgcgcat gttctggtac
    41161 gtgggactga acaaaaatgg tgcttttctc aggatcgata ccacaagcca gatacaaggc
    41221 cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg
    41281 ttggtcaacg atacagtaaa tgcaatggta gtcatcctgc atgtttaccc actgacgcag
    41341 cgcacccatg tagttaccaa tggtcaattc acctgagggc tgtgcgccac taaaaacgat
    41401 gggcttagtc atttttcgat tcctgatttt cgctatgcgg aagccctaat gcgggcagaa
    41461 ggtcatttat agactgataa attacatcag gctggctgag atcgatagcc tcgccgtagt
    41521 tatatccgta ggttaagcca actgatgggc aacctgccgc ttttgctgcc tgaatatcat
    41581 tgcgtgagtc gccaacaaac agcatctgtt gtggggcaat tcccatccgc tcagccacca
    41641 gtaacagcgg gtccggatgc ggttttttgt tttgcacatc atcaccacca atcaccacgc
    41701 tgaagtattt agcgatatct aaggcttcca gcagcggcgc gacgaacggc gtcggtttgt
    41761 tggtgaccag gcccaacggc aggcctttag cctgcaatgc gcccagcgta tcggcaacgt
    41821 gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata gtagcgatcg aacagtttac
    41881 gcagaatacg tacttgttct tctgccggaa tgtcgtcatc aacgggcggt ttacccattg
    41941 ttttacgcag agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc atcagaacat
    42001 ctgcgccgtt accaatccag gtaataacgc gttcttcacc tgcaacaggc aactccagcg
    42061 catacagcgc catatctacc gcagcagcaa gaccaggagc actgtccacc agcgtaccat
    42121 cgagatcaaa agcgacgccg cgaatatctt caaacttatt catgacttac ctttgccagt
    42181 tcactgcgca tttcatcaat gacttttttg tagtctggct ggtcgaagat tgccgaaccg
    42241 gcaacgaaca tatccgcacc cgccgcagcg atttcgccaa tgttgttcac cttcacgcca
    42301 ccgtcaactt ccagtcgaat gtcaaagcca gactcgtcga tacgacgacg tacttcgcgc
    42361 agtttatcca gtgtttgcgg aatgaaagac tgaccgccga aacctgggtt gacggacatc
    42421 agcaggatca catccaactt atccatcacg taatccagat agctcagcgg tgtcgccgga
    42481 ttaaatacca gacccgcttt acagccattt tctttaatca gttgcagcgt gcgatcaaca
    42541 tgctcggagg cttctggatg aaaggtaatg atgctggcac cagcggcagc gaaatcaggc
    42601 acaatgcgat cgacgggttt caccatcagg tgtacgtcga taggggcggt aatgccgtag
    42661 ttacgcaagg atttcagcac cattggccca atagtcagat tgggaacata gtggttatcc
    42721 atgacgtcaa aatgcacgac atcagcgcca gctgccaggg cttttgcggt atcttcaccc
    42781 aggcgggcaa aatcagccga cagaattgag ggggcaatca aatactgttt catccgcttc
    42841 tccttgagaa ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt
    42901 cgtccacctt tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact ttgacgacat
    42961 gcaattttgc gcgctgatac cactcacgcg ttaacatcgt atcgtgattg gagatcagca
    43021 ctggaatatg gcgatcaacc agaccttcgg cgatctccgc cagatgcgct tgttgttcaa
    43081 gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt cgcagacagc ggcgcataag
    43141 gcggatcgca atagacgacg gatgcatcat ctgcgcgcgc catgctatcg gcgtaagact
    43201 cacagtagaa aaaggcattc tgcgcttttt cagcgaagtg atacaactct gcttccggga
    43261 aatagggttt tttgtagcgg ccaaacggca cgttaaactc accgcgcaga ttgtaacgac
    43321 acaggccgtt gtaaccgtag cggttcaaat ataaaaacag taccgcccga cggaacggat
    43381 cctggctttt gttgaactct tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg
    43441 gaataaacag ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc acaatgttat
    43501 agagactgat caggtcgcta ttgatatcgg caaggatata acgagaaaag tcggtgttga
    43561 gaaacaccga cccggcacct acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc
    43621 gtttaatatc atcaagcagg ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt
    43681 tcttcatgct gactaactaa ttacaccttc tccggctgtg gagaaagctc cgacagcatc
    43741 cagcgcttca ggcaactccc gcaacgataa accattgcgg gagataacct tgattacttc
    43801 agatcggcct gtacctgacg cagcggtttc gcccacgggt ttttggcctg aacatctgct
    43861 ggcaatgtag ataccgcttt tttcgcctct tctttcgaag cgtacacgcc agaaaccagg
    43921 acataccacg gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt cagattctct
    43981 ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa
    44041 tggctggacg gtgccgattt caacgaacca acattacctg cggtcttccc tccagcggct
    44101 ggtgttgccg tggtttgcgc cggggatgcc gtctgtactg gagccgtagt tgcggtctct
    44161 tttggtgctg gcgtagaggt cgcagccggt gccttcgtgc ttgccacagg agcagctggt
    44221 tcagtacgct tcggcgtctg tgctaccggc ttcggctccg tttttacggt tgcttgcggt
    44281 tttttcggtt caatcaccgc ctgctggcga gctggacgcg tagtggccgg acgttcagcg
    44341 gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc gaacaggcgc gaccgtcgcg
    44401 ggttcagtcg gcaacgtgga attgaccgcc acattgttca actgttgctg attttgtggc
    44461 tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc
    44521 gccggggttt gcccttgagt cggcgtagaa gagatcggcg gcagagatac atcctgctga
    44581 gtattttccg cagacgtggt tcccggcgct ggctgcacgc cattcgcctg atcggtcgca
    44641 ttgccagcaa gatcaatact cttctcgcca gacgcagttt gatcgctgga agaggtcgag
    44701 ggggctttta gcgcagaacc gataccgata atcaacagca ggagaaccag aatgccgacg
    44761 cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgcgga
    44821 cgacgctcta cgcgctcttc atccacggtt tcatcttcgg attcgtcaat ttcttcttcg
    44881 agttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca
    44941 tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt
    45001 tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct
    45061 tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa
    45121 ttaacctgaa gctaatagac cgcttgataa gcggcctgac ctttcttgtt gttacgctga
    45181 ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact
    45241 cttaccaatt gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg
    45301 cagcatgtgc ggtaaatacg cctgcgcaga catttcgcgc ggcccattga ccggtaaccc
    45361 agcccgcgtg agcagggtta taatacgctg cgtttcggca gaactaaact gcccgagacg
    45421 ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcttcaccgt gtaaccaatt
    45481 gccatacccc atttcagctt caatggcatg accaaaggta tgtcccagat tcagtaaagc
    45541 acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg
    45601 gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc
    45661 cagccagttg aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc
    45721 cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc
    45781 aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc
    45841 gccaacggag gaatcgacct gcgacagcaa cgtcgtcggg acttgaatga aacgtacacc
    45901 gcgctgataa ctcgccgccg caaagccggt cagatcgccc actacgccgc cgccaagcgc
    45961 caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag
    46021 tacagccagg cttttatact gctcgccgtc agggaggata acgctatcga cgttaacacc
    46081 cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg tttcgttggt
    46141 gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa
    46201 accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat
    46261 aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag
    46321 ttgctttcca gcatgtgaat aatctggttt gcgaccactt tagcgctttg atcatcagta
    46381 cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc
    46441 agaacttcac gcggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg
    46501 cgtgcaagtt gcttttcgat ggtcgtttca agataaacga caacgccacg tgcggaaaga
    46561 cggttacgcg tttcgcggga tttcacagag ccgccgccag tagccagcac aataccctgt
    46621 ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct
    46681 aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg
    46741 tagaattcca tattgagttg ttgagctaac tggcgcccaa tagtgctttt tccggcaccc
    46801 ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta
    46861 ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac tgaaacctca
    46921 taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg
    46981 aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc
    47041 gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga
    47101 atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca aatcggtact
    47161 ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc gcaaaaacat
    47221 taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa
    47281 ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc
    47341 gccaagcaac ggtacgctat cctgacccga tttattttta cgggtaaaaa tgccacccag
    47401 cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc
    47461 cagcacttcg ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa
    47521 tttcagccgg atgcgacctt tttgtaacac cgtgggcgta acttccatcc ccaggacggc
    47581 ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaaacctgat atggaatttc
    47641 gctcccctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactggcgat
    47701 aatatccagc tgctgttttt gttcgagcgc ggaaagctca agatccagta aacgtccgtt
    47761 gatgcgtcca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctgcc
    47821 aagcgtggtg acttgcccaa cgccaccagc ttgttgcgca tcagccaacg tccatttcac
    47881 gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg
    47941 cccgaccggc agatccattt gcgctaccca ctgttcaagc gcgcttaacg ccgctttgtt
    48001 atctcgcagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag
    48061 tagcttctcc cccgctttcg ccagctctcc ggcgtcggcg tattgcagag taatattgcg
    48121 attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcctcctggc gggcgatatt
    48181 gtcattctgc caggcaacgg aatgcaccga gagaatattg ccttcctgcc gcgttatcag
    48241 tccggcgctt ttcactacag tttgtagtgc ttgcttccag ggaacatctg ttagatgtaa
    48301 cgacaccgta cctctgacgt ctggcgacac caccaggttc aacttctcct gttcagccag
    48361 cgcctgcaac acctgagcta ccggaacgtc atccaccatc agtgtcactt tttgcggctt
    48421 tgccgcctgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt gcttcatttg
    48481 tatctccttg ccgttgccac aaccagtgtg gcggttcgca gtttgttccg gttcccagcg
    48541 ttagtgcgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca tcgttttgct
    48601 gcacccgtcg ccatttcttt tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc
    48661 ctactatccc ctgatagcgc cactggctaa gctcgctaat ccggcataga tcttccggcg
    48721 gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc
    48781 gtttaaccct catccggtgt ctccagttgt agcgtgaaca aaaggtcatc accttccacg
    48841 cttaacgaaa aacggctcac gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc
    48901 actgcttccc acagcgtttt caacgccaac tcacctccct gcgcggatgg atgccaggaa
    48961 accagttgcg cgccggataa ctgaaaatcc agtggcgaaa agggcagcgt tttttcttcg
    49021 ctgaagggag tggtgtctac caggcgatac agtgcggccc actgctggtg atgactcgcc
    49081 cgtagtcgaa ttaatgcgtc gctctcttcc tggtgtgtcg atgacagaaa aatgagcgta
    49141 actaacatca gcaaccagaa tgcccagcaa agctggcgga ggcggggtga tgtggcgaac
    49201 caccagtcaa agagcatgtc catcgctaac cttccttgtt aactgatact caaattgcca
    49261 gcgtccctgc gcatcctgct gcgtggcccc ccgctgattg agatgaaaag atgcatcctg
    49321 gcggagtgac gtttccagcg cgtttaacgc ggtaatgctt gttgtcagcc ccttgatctc
    49381 cagcgttccc tgctgccagc ttatcgttgt cagccaggca tgttcgggta aaagcgccgc
    49441 cagtgcttcc agcgcagatt gccagtcacg ggtaaattgt cgctggagct gcctttgcaa
    49501 acgctgttcg cgtaactgct ggcgttccag caaacgtggt ttcgttatct gtaagctgcg
    49561 ggcgagttgt tgttccgcct gaagcaaaac ggtgtctacg cgtgcttcgg cgttgcttgt
    49621 cagacgcagt attagcgtta tcccgacagc cagcagcaga ggcgcaacga acatcagcaa
    49681 ccagaaacga agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg
    49741 gttcatcagt acaccttccc aagcgccagc cccagcgcga tggcaaagtc tccacctggc
    49801 ggcggtagcg gcggctgacg aacagaaacc gcctcccagg ggtcaaatcc gccttcgcca
    49861 catatcgcga cgctttcggg atcaacggat aacgctgccg ccagctcctt cgcgctagtt
    49921 atccccaccg ccagtttgcg cccccagcta tagcgcgtcg cccacagcca ctgttcgtta
    49981 tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaatcg ctgtaatgca
    50041 ctggcatccg gggtgatcgc gctaacatga acacgcaacc tttctgccag agtaagcagc
    50101 gttgccagct ctttgctttg cgcggcagtc acgttatagg cgggactgag tgagtcttcg
    50161 ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgggccat cgtcccggac
    50221 agccaggccg tttgctcccg ctcaccaagg gacatcgacg ggcgtggaaa tgaccgctgt
    50281 aatgtgcgac tggcgggaaa tgccaacata atgtgatgac gctgcggcag ttcgcgactc
    50341 cacggtaaca acgttttagc cagccgctgt gcatcgacaa tccgcccatc tttgattatg
    50401 tcgttctcca gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgt gccccgcacg
    50461 atagcaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca gatcttaaat
    50521 gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat gcgtctggct
    50581 tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa atgggaaatt
    50641 tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc tgctgggagc
    50701 aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg tggcgacatt
    50761 aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg agctgattgc
    50821 tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccgc cggagatggt
    50881 gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg ttgacccggt
    50941 ggggatcttc cgtgcggcaa gcgtggcgct gttctccggt cacgcgtcac aaggggcgag
    51001 taccattacc cagcagctgg cgagaaactt cttcctcagt ccagaacgca cgctgatgcg
    51061 taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga aagacgagat
    51121 cctcgagctt tatctgaaca agatttacct cggttaccgc gcctatggtg tcggtgctgc
    51181 ggcacaagtc tatttcggta aaacggtcga ccaactgacg ctgaacgaaa tggcggtgat
    51241 agccgggctg ccgaaagcgc cttccacctt caacccgctc tactcaatgg atcgtgccgt
    51301 cgcgcggcgt aacgtcgtgc tgtcgcggat gctggatgaa gggtatatca cccaacaaca
    51361 gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg agattgcttt
    51421 ctctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt atggcgaaag
    51481 tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtac agcaggccgc
    51541 acagcaggca gtacgtaata acgtgctgga ctacgacatg cgccacggct atcgcggccc
    51601 ggcaaatgtg ctgtggaaag tgggcgaatc ggcgtgggac aacaacaaaa ttaccgatac
    51661 gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca gcgccaatcc
    51721 tcaggaagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta tggaaggcgt
    51781 tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc gtaaagtgac
    51841 cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg catggtggct
    51901 ggcacaagtg ccggaagtga actcggcatt ggtgtcgatc aatccgcaaa acggtgccgt
    51961 tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg ccacccaggc
    52021 actgcgtcag gtaggttcca acatcaaacc gttcctctac accgcggcga tggataaagg
    52081 tctgacgctg gcaagtatgt tgaacgatgt gccaatttcc cgctgggatg caggtgccgg
    52141 ttctgactgg cagccgaaga actcaccacc gcagtatgcc ggcccaattc gcttacgtca
    52201 gggactgggc cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga tgggcgtcga
    52261 ctacgctgca gaatatctgc aacgcttcgg cttcccggca caaaacattg tccacaccga
    52321 atcgctggcg ctgggttcag cgtccttcac cccaatgcag gtggcgcgcg gctacgcggt
    52381 catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg aaaacgatca
    52441 gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgcg atattccggt
    52501 gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg aagatgtcgc
    52561 tatctcccgc gagcagcaga atgtttctgt gccaatgccg cagctggagc aggcaaatca
    52621 ggcgttagtg gcgaagactg gcgcacagga gtacgcgccg cacgtcatca acactccgct
    52681 ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgagccag gctggcaggg
    52741 tactggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga aaaccggtac
    52801 cactaacagt tcgaaagatg cgtggttttc gggttacggt ccgggcgttg tgacctcggt
    52861 ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt ccggagcgat
    52921 taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccagcctg catgggacgc
    52981 ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc cgccgggtat
    53041 tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca acagccgcga
    53101 agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg tgggaacgac
    53161 cattatcgat aatggcgagg cacaggaatt gttctgatta aaaaggcgct tcagcgcctt
    53221 tttcagtttg ctgacaaagt gcactttgtt tataccggat acggcgtgaa cactttaccc
    53281 gtctagcaac atctagaaaa atcaatatat tgcaggagcc gcacaggcct gataagcatt
    53341 gtgcatcagg caaacctcac gcatttacac tcgcccctgc cccttcaacc attcgcgcac
    53401 gaggaacagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca gcaaatccat
    53461 catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatctc cttccagcga
    53521 ttccgggtag agatcttgcg ctaccacgat attcattttg ctggaaaagt aagacggtgc
    53581 catactgagc ttcttcaaaa aagtcagatc gttcgcacca aatcccacct cttcttttag
    53641 ctcgcggttc gcggcttcga agacgctttc acccggatca attaatcctt tcgaaaaacc
    53701 taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt gatcgtccac
    53761 aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat aaacacgccg
    53821 cacgccattg ctgaactcca gatccacgct ttcgacggta aacagtcggg aacgggctac
    53881 agtttcaaca ttcagaatgg tgggtttttg taatgatttg ctcatcgtgg gatctatgct
    53941 gtgaaatcag cggttattgt gcgatatagg acacgctttc ggcaatgtga attgcatgtt
    54001 atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa gtcaataggt
    54061 tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta tgatcagcgg
    54121 ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat agccaaccgc
    54181 agaatcatgt attgtgtccg gtgcgactga ccacgcctga cagactaagt aagatgggga
    54241 aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac tacttgcggg
    54301 atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg ccttcgcgga
    54361 tgcgcaaacg cgtaaactca cacctgaaga acatagcgcc gttgaaaatt atcttgagag
    54421 cctgacgcag gtattacagg tgcctggccc aacgggagcc agcgcggcac cgatctctct
    54481 ggcgctgaat gccgaaagca acaacgtcat gatgctgaca cacgctatca cgcgttacgg
    54541 catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag aagtccacct
    54601 gccccctttc tgggagcagt acatcaacga tgagaatacc gttgaactga ttcataccga
    54661 ttcgctgccg ctggttattt cactcaacgg tcatacgctg caggagtaca tgcaggaaac
    54721 tcgcggctat gccttgcaac ctgttccgtc aacacaggcg tcgattcgcg gggaagagag
    54781 tgagcaaatc gagctactca atattcgcaa agaaacgcac gaagagtatg cgctaagtcg
    54841 tccgcgcggg ctgcgcgaag cgttgctgat tgtcgcctcc ttcctgatgt tctttttctg
    54901 cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcgttac tgctgctggg
    54961 cgcaggactg tgggggctat tcgcgccccc ggcaaaatcc tccctgcggg aaattcattg
    55021 tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg aacagatcaa
    55081 caatatttca ctcggtatta tcgacctggt ctaccccgca cactggcagc cctacattgc
    55141 tcaggatctc ggtcaacaaa ccgatatcga tatctatctc gaccgccacg tagtgcgtca
    55201 ggggcgatat ctttcgctcc atgatgaagt aaaaaacttt ccgttacagc actggctgcg
    55261 cagtacgatt atcgctgcgg gctcgctact ggtgctgttt atgctgttat tctggatccc
    55321 gctggatatg ccgctgaaat tcaccctctc gtggatgaaa ggcgcgcaga ccattgaagc
    55381 caccagcgtg aaacaactgg ctgacgctgg cgtgcgggtt ggcgatacat tgcgtattag
    55441 cggtacggga atgtgtaata ttcgcacttc cggtacctgg agcgcgaaaa ccaactcacc
    55501 ttttttaccg tttgactgct cgcagatcat ctggaatgac gcccgctcat tgccattacc
    55561 agaatctgaa ttggtcaaca aagcgacggc attgaccgaa gcagttaatc gccagctgca
    55621 ccctaaaccg gaagatgaat ctcgcgtcag cgcctcatta cgttcagcaa ttcaaaaatc
    55681 cggcatggta ttgcttgatg attttggcga catagtactg aagacagcgg atctgtgttc
    55741 tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggta acagtaaaga
    55801 ctgggacgcg ctggtaaaac gcgccaacgc cgggaaactc gatggcgtga atgtgttatt
    55861 acgcccggtg agtgcggaat cgctggataa cctggtggcc acctctaccg cgccgtttat
    55921 cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg gcgggttcct
    55981 gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg catcacttta
    56041 cgactatccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa tgctgatgca
    56101 tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc actgacgcca atggtacgca
    56161 gcatattggc cttcatccga tcccggatcg ttccggcctg tggcgctatc tcagcaccac
    56221 gctgctgcta ctgacgatgc tgggtagcgc catttataat ggcgtacagg cctggcgtcg
    56281 ttaccagcgt catcgtactc gcatgatgaa gattcaggcc tattatgaaa gctgcctgaa
    56341 cccacaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg cggcaggtta
    56401 tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat atcaacattg
    56461 cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctc gacctcgcct
    56521 tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa aacggggtta
    56581 cgccacagga agcgagggaa tatatgcgcc agcaatatca cgacgtacag catacgctaa
    56641 actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt gcgatgacca
    56701 ccgagatggg accgcgcgcc gtactgcgtg aagataccat tccgtttctt gaggcactga
    56761 aagccagcgg taagcagcga attttgctca ccaatgctca tccgcacaac ctggcggtaa
    56821 aacttgagca taccggtctg gacgcacacc ttgatttatt actttccacc cacacatttg
    56881 gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg ggactgaaag
    56941 ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc gcgcaatttg
    57001 gtattcgtta ctgcctcggc gtgactaatc ctgactccgg gattgccgag aaacagtatc
    57061 aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg tgaaggagac
    57121 gccatgaaag aaaaacctgc tgttgaggtt cgactggata aatggctatg ggctgcccgt
    57181 ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag gcggtaaggt gcattacaac
    57241 gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac tctgcgccag
    57301 ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg ccccgccagc
    57361 gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga aaaaatggcg
    57421 ctggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc ggacaaaaaa
    57481 gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc acctgcaaga
    57541 gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa ctttgccgtg
    57601 cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa tcacgattat
    57661 ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct gttaaccgct
    57721 acgctgaagt ttgatggtga tatcaccgta cagctgcaag gcgacggtcc gatgaatctg
    57781 gcggttatta acggtaacaa caaccagcag atgcgcggtg tagcgcgcgt gcagggagaa
    57841 attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt gatcaccatc
    57901 accccgagcg aaggtgaacg ctatcagggc gtggtgggtc tggaaggtga tactctggcg
    57961 gcctgcctgg aagattactt tatgcgttct gaacagctgc cgacgcgcct ttttattcgc
    58021 accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt aatgcctgcg
    58081 caaaatgccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac catcaaaacc
    58141 gaagaactgt tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca cgaagaagag
    58201 gtgacggttt acgatccgca ggatgtggag ttcaaatgca cctgctcgcg tgaacgttgc
    58261 gccgacgcgc taaaaacgct gccggatgaa gaagtcgata gcatcctgac ggaagatggc
    58321 gaaattgaca tgcattgcga ttactgcggt aaccactatc tgttcaatgc gatggatatt
    58381 gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg agtatgtccg
    58441 gcagagaggg ctctgccgga ttttacgact aatacacctg atgcgctttg tcactcattc
    58501 agattcacat ccagcctttc accatccact acaagatcgc gccacgcttt aggcttcgtt
    58561 cccagttttc catccttcgc agctgtgagt aatttttctt tcgttatatc acgcggaagt
    58621 aaactcatcg taagctcttg ccattctttc ttgtcaggat caaagccata gacaatgtat
    58681 ctttcatcat caaaggcaaa gaggatccgc tctgccctgc catcactatt cagatcttgc
    58741 tcgaccagta cgcaggcgtc tttttcaata caggtcatca cgttatagcg atcctggatt
    58801 aaggccgacc agaatgtcgc atccggttta ccagaaccag gggcaattaa cacattttcg
    58861 gctaatactt tttccgatat ctgttgctga agatgttgct ctccatctaa cgccattaac
    58921 agatcccgcg cgcgtttcgg gtctttcata aacccggcat cgcttttcag cgactcaagc
    58981 gccgcacgtc catagcgacc gctctgttcg agcatataga gacttacctg gtctggcgtg
    59041 tttttgccgc tctgataacg cgccatatga ctgttcacgc taatgcgcat actgtccagc
    59101 accggagaat taagaagcac cagtattacc aacaccaata acgaaaccgc aaggttcact
    59161 ttgccctgaa gaacaagggg gttttgccct ttacgccaga cgatactgac aaaatacccc
    59221 agcgaccata ccagtaacac cagcaccgcc agcgcaccct gcaagcggtc gacagtccag
    59281 ccgtactgag cgacccgcag ccataacgcc caggcggcga cgaacacata cagcggagca
    59341 accagcagag cggttttaat caggcaacgc aacggccccg tccagggaag tgacgatttt
    59401 tgcggatcgc ggacaatagc cattaagatc aattgcaaaa aggccagcgt caacagcagc
    59461 ccggcggcgg agatgtggcg agaaatcgcg ctcaggcccg taaacggcag ggtgatgata
    59521 aacatcaggg ttagcaatga tactaacggc agcaaccccg tggcgattaa cgttaacaac
    59581 ttttgaatag agtcgattaa acgtgactgt gttcgcgcga ggatcaccgc cagcgcggta
    59641 accagaccta acgtgagata aataaaccag tcggttgcaa aaaagagcgt tttgaaaaac
    59701 gtgatgccaa caagtttaaa caactcgccc cacagtaaaa gcaccagcca cgtcaggcca
    59761 ttggcgagaa aaatcaccag taatataagt acgttatgcc atactgactg gtaaaaatag
    59821 ccatagcggc tactgtcatt gcggatgcga aggctttgtt gtatccacgg tagcaacaac
    59881 attgccatca gcagcagata gcagccaaaa tcccaaagcg ccttttctgc tctccagggg
    59941 ttcatgccat caatttgcca ctttagccaa ccgctcatcc ctagcgtggc aataaacacc
    60001 agcgccagcc atccccaaag gcgcttctgt ttaaaggaga tcacggagaa gagtaaaacg
    60061 gatgaaaatg cgacggtcgc aggcacgcca taaacaatcc aactgagatt ttttccggca
    60121 agccaggcga tcagtaggta gcaaaccaga ccctgaagta atccggtagc aatcatcccc
    60181 cagcgtgttg cgggtgaaag ttcaacgtta tccacgttag ttatccagaa tcaaaaagtg
    60241 ggttaattat cgcatccggg ccgtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    60301 ttagcgtggt gaatcgatac tttaccgatt gaatttgcat caatttcatt caggaatgcg
    60361 attccactca caatattccc gccacataaa ccaagattta accttttgag aacattttcc
    60421 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    60481 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    60541 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    60601 ttaacaatgg tttgaccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    60661 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    60721 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    60781 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    60841 ggtgggcaga caaaggcaaa ggtaagaacg ataacaaacc tctctctccg gaaacctggc
    60901 agcatctgaa gggcctggtg accaaacagc tttccggcaa acgtctgttc gttgtcgacg
    60961 ccttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    61021 ggcaggcgca tttcgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    61081 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    61141 agggtctgaa ttccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    61201 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcaatgatg aactacctgc
    61261 taccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    61321 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    61381 gtcgcctgat tggcgatgac gagcacggct gggacgatga cggcgtattt aacttcgaag
    61441 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    61501 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    61561 ttgatgatgg ttcaaaaacc gagaacactc gcgtttctta tccgatctat cacatcgaaa
    61621 acatcgttaa gccggtgtcc aaagcgggcc acgcaactaa agttatcttc ctgaccgcag
    61681 acgcttttgg tgtgctgccg ccggtttctc gtctgactgc cgatcaaacc cagtatcact
    61741 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    61801 ccaccttctc cgcttgcttc ggcgcggcat tcctgtcgct gcacccgact cagtacgcag
    61861 aagtgctggt gaaacgtatg caggcggcag gtgcgcaggc gtatctggtt aacaccggct
    61921 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    61981 tcaacggttc gctggataac gcagaaacct tcactctgcc gatgtttaac ctggcgattc
    62041 cgaccgagct gccgggtgtt gacacgaaga tcctcgatcc gcgtaatacc tacgcttctc
    62101 cggaacagtg gcaggaaaaa gctgaaaccc tggcgaaact gtttatcgac aacttcgata
    62161 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaag ctgtaatgat
    62221 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    62281 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cacaggcagc
    62341 caggcgcgaa tggaaagccc gccccgttcg ctggtgccaa gctccagcat cccgttatgg
    62401 ttatccacga tacgctgcac aatcgccagc cctaatcccg tgccgctaat ggtgcgcgca
    62461 ctgtcgccgc gaacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    62521 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    62581 cagccattgc cgtaacgggc ggcgttgacc accatattcg ccaccgcacg tttgatcgac
    62641 agcgggtgca ttttcacctc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    62701 tagccacttt cggcggcaat cacctcaccg agcactgcgt tgagatccgc catttccatc
    62761 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    62821 tcgatatctt tattgatcga ttctgccagg tagccatcct gctcgctcat catctcggtt
    62881 gccaggcgaa tgcgcgtcag cggcgtgcgc agatcatgac ttacccccgc catcagcagc
    62941 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc gcgggtaacc
    63001 gaacgcacct cggacgcgcc atattcacgc agcggcggcg gaataattcc tttaccaacc
    63061 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacaaccac
    63121 gccccgccta tcgccaatag cataatcgcc aacgtatagc ggaacagcgg agaaaaatcg
    63181 ccctgatgaa tttcggtcag cggcacgcgc acccagatat tgggcgacag ccaggttttc
    63241 agccagacga caggcgaact tttgttgacc tcaacgcgta cttccgtcgg gccgcccagt
    63301 tgctgcgcca tctgatggct taagaattca tagtgttgtg cccaacgcag acctgcctct
    63361 tcagcagctt cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    63421 gcgggaggca caaccaactg cgtaccgtcc tccagttgca gtttgtcggt catcaacata
    63481 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    63541 agcaccacca gataagtcgt caccaggctg acgaacagca aggtgacgat gagcaataac
    63601 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gaaccgtccg
    63661 gtacaaagac gtagcccaga ccccagacgg tctgaatgta acgcggatgc gccggatctt
    63721 cttccaccat gcggcgtaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggagt
    63781 attcacggcc acgggcaagg ttcataagct tatcgcggga gagcggctca cgcggatggc
    63841 tgaccaatgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    63901 ggaacatttc acgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    63961 cctgtgacgg cgcgcccggc agttcgttgg cctgacgacg cagcaccgca cgaatgcggg
    64021 ccagcaattc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    64081 caacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    64141 tctggctacg aagacgtcgg caaatcgaca agccatcttc accaggtaac atcaaatcca
    64201 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    64261 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgcg cgcaggcgca
    64321 tatcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    64381 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    64441 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    64501 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    64561 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    64621 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    64681 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    64741 aaaaagcgcc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg catggaaaat
    64801 ctcaaaatcg tcgattactc cccgcagcag gaaggcaaag tcttttttgg tgcgtgggtg
    64861 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    64921 tttggtcgta aagattacat ctctatcgat tccccgatgg ctcgcgcatt gttgaaaaaa
    64981 gaggtgggcg atttggcggt ggtgaatacc cctgccgggg aagcgagctg gtatgttaat
    65041 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    65101 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    65161 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    65221 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    65281 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    65341 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    65401 ggcaaactca ctgacgagct ggcgaatgcc atcaacgcca cactaagcaa aaccgaactc
    65461 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    65521 gcagggcttg agccgttggc tgacctgctg tggagctctc catcacacac gccagaagtc
    65581 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggac
    65641 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg
    65701 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagtgg taaagaagag
    65761 gaaggggcga aattccgcga ctatttcgat catcacgaac cgctgtcaac ggtgccttct
    65821 caccgcgcgc tggcgatgtt ccgtggtcgt aacgaaggcg tacttcagct ttcgctgaat
    65881 gccgatccgc agttcgatga gccgccaaaa gagagctatt gcgagcaaat cattatggat
    65941 catctcggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaagg cgtggtgagc
    66001 tggacgtggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg caccgtgcgc
    66061 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    66121 gcggccccag ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    66181 aaagtagcgg tggtcgatgc aactggcaaa ctggtggcga ccgataccat ttacccgcac
    66241 accggacaag ccgcaaaagc agcgatgacc gttgctgccc tgtgtgaaaa gcataatgtt
    66301 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgagcgttt ctatctcgac
    66361 gtgcagaagc agttcccgaa agtgaccgcg cagaaagtga tcgtcagcga agcaggcgcg
    66421 tcggtttact cggcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    66481 cgtggcgcgg tatctatcgc ccgtcgtttg caagatccgc tggcggagct ggtgaaaatc
    66541 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcagctggcc
    66601 cgcaaactgg atgcagtagt agaagactgc gtaaacgctg ttggcgtcga tctcaacacc
    66661 gcttctgttc cgctgttaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    66721 gtggcctggc gcgatgagaa cggtcagttc cagaaccgtc agcaactgct gaaagtgagc
    66781 cgtctggggc cgaaagcctt cgagcagtgt gcgggcttcc tgcgtattaa ccacggcgat
    66841 aacccgctgg acgcctctac cgttcacccg gaagcctatc cggtggtgga acgcattctg
    66901 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgagct gcgtaacctg
    66961 aaagcgtctg actttactga tgagaaattc ggtgtgccga cagtaactga catcatcaaa
    67021 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgctgat
    67081 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc ggtgaccaac
    67141 gtcaccaact ttggcgcgtt tgtcgatatc ggcgtgcatc aggacggcct ggttcacatc
    67201 tcttcattgt cgaataagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    67261 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gaccatgcgt
    67321 ctggacgagc agcctggcga aaccaacgcc cgtcgcggcg gcggtaatga acgcccgcaa
    67381 aacaaccgcc cggcagccaa accgcgtggg cgtgaagcgc agcctgccgg taacagcgcg
    67441 atgatggatg cgctggcggc ggcaatgggc aaaaaacgtt aaacgccctg gctggcctac
    67501 ggttcaaatt tgcacgaaat cgtagggcag ataaagcgtt cacgccgcat ctggcaacga
    67561 acgccttgcc tgacataaaa gtgccggaga atatctccgg cctttttatt ccacagccaa
    67621 actcacaata tatttcgtta atatttatca cttcattaac aactgaaacc ttaattaaac
    67681 attaaccagt cctggtaatt cgctattcga attatatttt cgctgcgata taaccttgag
    67741 ccacatcaac attgagtcag attattattc aaaccaacat tcgcacacat tttaagtatt
    67801 gctgatagaa accattctca ttatcattgt gttgttgatt atttaatctc tcctttgttg
    67861 gcaaatcatc tggtctcatg tcgctgtcaa acgccccatg aggtagttat ccagttaatg
    67921 agaaacaagt aggcacctat gcaatacact ccagatactg cgtggaaaat cactggcttt
    67981 tcccgtgaaa tcagcccggc atatcgccaa aaactgcttt ctcttggcat gttacctggc
    68041 tcctctttta atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt
    68101 cgtgtgagcc tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc
    68161 tgttaatacg gtgataacaa caatgaaaaa attaaccatt ggcttaattg gtaatccaaa
    68221 ttctggcaag acaaccttat ttaaccagct cactggcgca cgtcagcgtg taggtaactg
    68281 ggctggcgtt accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac
    68341 gctggtggac ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga
    68401 tgagcaaatc gcctgtcact acatcttgag tggcgacgcc gacctgctga ttaacgtggt
    68461 ggatgcgtct aaccttgagc gtaacctgta cctgacgcta caactgctgg aactcggcat
    68521 tccctgcatc gtggcgctga acatgctcga cattgccgag aagcaaaata ttcgcattga
    68581 aattgatgct ctgtcggcgc gtcttggctg cccggtaatc ccgctggttt caacccgtgg
    68641 tcgcggtatt gaagcgctca agctggcaat tgatcgctat aaagctaacg agaatgtgga
    68701 actggtgcat tacgcacagc cgctgctcaa cgaagcagat tcactggcaa aagtgatgcc
    68761 ttccgacatc ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat
    68821 ctacagccgc gcctacgccg gtgaagcgtc gcagcatctt gatgccgccc tcgcccgtct
    68881 gcgtaatgag atggacgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc
    68941 tgccatctgt gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc
    69001 ggtagataaa atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta
    69061 cctgatgttc ctgctggcta tcaacatcgg cggggcgtta cagccgctgt ttgacgtcgg
    69121 ctccgtggcg ctatttgtgc atggtattca gtggattggc tacacgctcc acttcccgga
    69181 ctggctgact atcttcctcg cccagggcct gggtggcggt attaacaccg tgctgccact
    69241 ggtgccgcag atcggcatga tgtacctgtt cctctccttc ctcgaggact ccggctatat
    69301 ggcgcgtgcg gcgtttgtga tggaccgtct gatgcaggcg ctgggcttgc cggggaaatc
    69361 ctttgtaccg ctaatcgtgg gtttcggttg taacgtaccg tcggtaatgg gcgcacgtac
    69421 gcttgatgca ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg
    69481 cgcgcgtctg gctatcttcg cagtattcgc ggctgctttc ttcgggcaga acggtgcgct
    69541 ggcggtcttc tcgctgtata tgctcggtat tgtgatggcg gtgctgactg gcctgatgct
    69601 caagtacacc atcatgcgcg gtgaagcgac gccgtttgtc atggagctgc cggtctacca
    69661 tgtaccacac gttaaaagcc tgattatcca gacctggcag cgtctgaaag gcttcgttct
    69721 gcgtgctggt aaagtgatta tcatcgtcag cattttcctg agtgctttca acagcttctc
    69781 tctgagcggg aaaatcgtcg ataacatcaa cgactcggcg ctggcttccg tcagccgggt
    69841 gatcacccca gtcttcaagc cgattggcgt gcatgaagat aactggcagg caacggttgg
    69901 tctgtttaca ggcgcaatgg cgaaagaagt ggtggtgggt acgctcaaca ccctctacac
    69961 cgcagaaaat attcaggacg aagagttcaa tccggcggaa tttaacctcg gtgaagagct
    70021 tttcagcgcg gtagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact
    70081 gatgaacccg attgaagcga gcaaaggcga cggcgaaatg ggtaccgggg cgatgggtgt
    70141 gatggatcag aaattcggta gcgcagctgc cgcatacagc tacctgattt tcgtcctgct
    70201 gtacgtaccg tgcatctcgg tgatgggtgc tatcgcccgt gaatcaagcc gtggctggat
    70261 gggcttctcc atcttgtggg ggctaaatat cgcttactcg ctggcaacat tgttctatca
    70321 agtcgccagc tacagccagc atccaactta cagcctggtg tgcattctgg cggttatcct
    70381 gtttaacatc gtggttatcg gtctgctgcg ccgcgcgcgt agccgggtga atatcgaact
    70441 gctggcaacg cgcaaatcgg taagcagttg ctgcgcagcc agcaccaccg gtgattgcca
    70501 ttaatggctt cacttattca ggtacgtgat ttgctggcgt tacggggccg tatggaagcg
    70561 gcccagataa gccaggcatt gaatacgccg cagccaatga ttaacgccat gctgcaacag
    70621 ctggaaagca tgggcaaagc cgtgcggatt caggaagaac ctgacggctg cctctctggc
    70681 agttgtaaaa gctgcccgga aggcaaagcc tgcctgcgcg agtggtgggc gctgcgttaa
    70741 ccttactcca tcgcctgttt tgaaaagccc ggtatgcgtt tgcatccggg ctttttgcgt
    70801 gcggctttcc ataaaaatgc aacccttgca acgcgacgta aattcctctg aaagcgtctc
    70861 gcagggatga aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca
    70921 aacaacaggt gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg
    70981 ttcaaactct ttttacgcca accggagacg gcgcgcgatt ttcttgcgtt ccatttaccg
    71041 gcacctattc acgcgctctg tgatatgaaa accctcaagc tggagtcgag tagctttatt
    71101 gatgacgatc tgcgtgaaag ctattccgac gtgctgtggt cggtgaaaac ggaacaagga
    71161 ccaggataca tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt
    71221 cgcatgatgc gctacgccat tgccgcaatg caaaatcacc tggatgccgg atacaaaaca
    71281 ttgccgatgg tggtgccgtt gttgttttac cacggtattg aaagccccta tccctattcg
    71341 ctgtgttggc ttgattgttt cgccgatccc aacctggcaa ggcagcttta tgcctctgca
    71401 tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg
    71461 gcgctgctgg agttaattca aaaacatatt cgtcaacgcg acctgatggg gctggtagag
    71521 caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa agggctgttt
    71581 aattacatac tgcaaactgg cgatgcggta cgttttaacg attttatcga cggtgtgacc
    71641 gaacgttcac cgaaacacaa ggagagttta atgactattg cggaaagatt gcggcaggaa
    71701 gggtttcaga aaggtatacg taccaacgcc ctaaatattg ccaaaaccat gctggatgcc
    71761 ggagtctctc ttgaggacgt tctgcgattt accagcctga cggttgaaga ccttgctgaa
    71821 ataaatcatc aataacgtaa aggatggttt taatgacaga tgcagaaaga gaatatctta
    71881 ttagttacct gattggttat ttattggatc gacgcctgga agccctggat atcgccaaaa
    71941 gaatgctgta atccggagtc cctcttgcag acatcatgcg ttttaccggg gtgtcagaag
    72001 aagagttggc tgcggcgagt cagtaaagtt ctgtctcgcc atttcaaaag ccacctacac
    72061 cctctgcttc aacgccacca gcatgcgaca aaactcggcc ggatgcgaaa taaacggcgc
    72121 atgggccgct ttggcgaaga tgtatgattc gctgtgaggc caaagtttat ccagcatcgg
    72181 caccactttg cgcggcacca gaccatcgag atagccatac aatcgcaaaa acggcatgga
    72241 cacgttttgc agcggctgac ggagatcgac cgttttcagg atttccagcc cgccattaag
    72301 cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc gcgcatcctg
    72361 gcgcgccgtt tcagtaccca tggtttgtaa cgcaaggaac cgttccactg tacgctgaaa
    72421 atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc ccggccactc
    72481 gtcacgagca ctaaaacaag gcgacgacgc cacggtgacc agtgcctgaa cacgctcggg
    72541 atgggttaac gcaatctggc ttgccaccag cccacccaga ctccagccta accaaatggc
    72601 tttatcaggt gcctgtcgca gcacggcttc ggccatatca gcaagcgaca gcgcaccaaa
    72661 tccccggctg cgcccgaaac cgggcagatc aacaaggtgc agcgtaaaat gcgagctaag
    72721 ttcctcgtca atgcaacgcc acacttcggc attcagtccc catccgtgca acagcacaag
    72781 atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct attgttctct
    72841 tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc tatgccgaat
    72901 gccgctggcg ttaggtcatt gggggatttg ttcggtctgt tcacgcgccg cccgtacaga
    72961 taaaacgtta tgcccacaat gtggattacc cgccacacac tcacatcttc cctgcggtcg
    73021 ctgcctgcaa aaacc
//
LOCUS       DAYPHN010000006        67570 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000006 DAYPHN010000000
VERSION     DAYPHN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.006"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1727
                     /locus_tag="ACG0BW_000387"
     CDS             <1..1727
                     /locus_tag="ACG0BW_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFK7257402.1"
                     /translation="VMAAGFKPAPPAGQLGAVIVDPYGNAPLTALVDLDSHVISDVKV
                     TVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQKFANKVTVEWKENGKVMKDDYVVH
                     TSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLVNTHTFTAQGSDLHWHGEKDKNA
                     GILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQDTFYDGRDRDINKRGYLMGIRET
                     PRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETPNGTVLLRVGKSNYRR
                     DDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGALDAGAVCVNVDLAHAG
                     QQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSRHQGVVKIGRDKQVKW
                     ILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSDFDFTYTQHTAWISSKGTLT
                     IFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGKERGYDFYSPITSIIE
                     YQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVKVEIDVLSDKPNQTHYRALL
                     VRPQQMFK"
     gene            1745..2413
                     /locus_tag="ACG0BW_000388"
     CDS             1745..2413
                     /locus_tag="ACG0BW_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFK7257403.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYEVAKIQGVPAYVVNGKYLIYTKSIKSIDSMADLIRE
                     LASK"
     gene            2428..3099
                     /gene="dsbI"
                     /locus_tag="ACG0BW_000389"
     CDS             2428..3099
                     /gene="dsbI"
                     /locus_tag="ACG0BW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFK7257404.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRFLWLLMAIAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3145..3933)
                     /gene="ygiD"
                     /locus_tag="ACG0BW_000390"
     CDS             complement(3145..3933)
                     /gene="ygiD"
                     /locus_tag="ACG0BW_000390"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFK7257405.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPQADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4076..4849
                     /gene="zupT"
                     /locus_tag="ACG0BW_000391"
     CDS             4076..4849
                     /gene="zupT"
                     /locus_tag="ACG0BW_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFK7257406.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4907..5077)
                     /gene="yqiD"
                     /locus_tag="ACG0BW_000392"
     CDS             complement(4907..5077)
                     /gene="yqiD"
                     /locus_tag="ACG0BW_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFK7257407.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5339..5992)
                     /gene="ribB"
                     /locus_tag="ACG0BW_000393"
     CDS             complement(5339..5992)
                     /gene="ribB"
                     /locus_tag="ACG0BW_000393"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFK7257408.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADDAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6096..6244)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6366..6665
                     /gene="ubiK"
                     /locus_tag="ACG0BW_000394"
     CDS             6366..6665
                     /gene="ubiK"
                     /locus_tag="ACG0BW_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFK7257409.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6700..6900)
                     /gene="glgS"
                     /locus_tag="ACG0BW_000395"
     CDS             complement(6700..6900)
                     /gene="glgS"
                     /locus_tag="ACG0BW_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFK7257410.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7169..7798
                     /gene="yqiJ"
                     /locus_tag="ACG0BW_000396"
     CDS             7169..7798
                     /gene="yqiJ"
                     /locus_tag="ACG0BW_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFK7257411.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7825..9486
                     /gene="yqiK"
                     /locus_tag="ACG0BW_000397"
     CDS             7825..9486
                     /gene="yqiK"
                     /locus_tag="ACG0BW_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFK7257412.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQSLPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGNAASGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVEEKTE"
     gene            9726..9785
                     /locus_tag="ACG0BW_000398"
     CDS             9726..9785
                     /locus_tag="ACG0BW_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFK7257413.1"
                     /translation="MMKLVIILIVLLLVSFQAY"
     gene            10103..10162
                     /locus_tag="ACG0BW_000399"
     CDS             10103..10162
                     /locus_tag="ACG0BW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFK7257414.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(10282..11715)
                     /gene="hldE"
                     /locus_tag="ACG0BW_000400"
     CDS             complement(10282..11715)
                     /gene="hldE"
                     /locus_tag="ACG0BW_000400"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFK7257415.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKTPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11763..14603)
                     /gene="glnE"
                     /locus_tag="ACG0BW_000401"
     CDS             complement(11763..14603)
                     /gene="glnE"
                     /locus_tag="ACG0BW_000401"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFK7257416.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELQSQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTVHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVVRYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14626..15927)
                     /gene="ygiF"
                     /locus_tag="ACG0BW_000402"
     CDS             complement(14626..15927)
                     /gene="ygiF"
                     /locus_tag="ACG0BW_000402"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFK7257417.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVSLSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPLVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            16169..16789
                     /gene="ygiM"
                     /locus_tag="ACG0BW_000403"
     CDS             16169..16789
                     /gene="ygiM"
                     /locus_tag="ACG0BW_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFK7257418.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            16853..18091
                     /gene="cca"
                     /locus_tag="ACG0BW_000404"
     CDS             16853..18091
                     /gene="cca"
                     /locus_tag="ACG0BW_000404"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFK7257419.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(18273..19094)
                     /gene="bacA"
                     /locus_tag="ACG0BW_000405"
     CDS             complement(18273..19094)
                     /gene="bacA"
                     /locus_tag="ACG0BW_000405"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFK7257420.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(19185..19553)
                     /gene="folB"
                     /locus_tag="ACG0BW_000406"
     CDS             complement(19185..19553)
                     /gene="folB"
                     /locus_tag="ACG0BW_000406"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFK7257421.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19658..20275
                     /gene="plsY"
                     /locus_tag="ACG0BW_000407"
     CDS             19658..20275
                     /gene="plsY"
                     /locus_tag="ACG0BW_000407"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFK7257422.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(20288..21220)
                     /gene="ttdR"
                     /locus_tag="ACG0BW_000408"
                     /gene_synonym="dan"
     CDS             complement(20288..21220)
                     /gene="ttdR"
                     /locus_tag="ACG0BW_000408"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFK7257423.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            21427..22338
                     /gene="ttdA"
                     /locus_tag="ACG0BW_000409"
     CDS             21427..22338
                     /gene="ttdA"
                     /locus_tag="ACG0BW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFK7257424.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAMLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            22335..22940
                     /gene="ttdB"
                     /locus_tag="ACG0BW_000410"
     CDS             22335..22940
                     /gene="ttdB"
                     /locus_tag="ACG0BW_000410"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFK7257425.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22989..24452
                     /gene="ttdT"
                     /locus_tag="ACG0BW_000411"
     CDS             22989..24452
                     /gene="ttdT"
                     /locus_tag="ACG0BW_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFK7257426.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPLLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(24495..25508)
                     /gene="tsaD"
                     /locus_tag="ACG0BW_000412"
     CDS             complement(24495..25508)
                     /gene="tsaD"
                     /locus_tag="ACG0BW_000412"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFK7257427.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25746..25961
                     /gene="rpsU"
                     /locus_tag="ACG0BW_000413"
     CDS             25746..25961
                     /gene="rpsU"
                     /locus_tag="ACG0BW_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFK7257428.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            26072..27817
                     /gene="dnaG"
                     /locus_tag="ACG0BW_000414"
     CDS             26072..27817
                     /gene="dnaG"
                     /locus_tag="ACG0BW_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFK7257429.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            28012..29853
                     /gene="rpoD"
                     /locus_tag="ACG0BW_000415"
     CDS             28012..29853
                     /gene="rpoD"
                     /locus_tag="ACG0BW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFK7257430.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(29932..30438)
                     /gene="mug"
                     /locus_tag="ACG0BW_000416"
     CDS             complement(29932..30438)
                     /gene="mug"
                     /locus_tag="ACG0BW_000416"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFK7257431.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            30563..30638
                     /locus_tag="ACG0BW_000417"
     tRNA            30563..30638
                     /locus_tag="ACG0BW_000417"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30596..30598,aa:Ile,seq:cat)
     gene            complement(30692..31456)
                     /gene="nfeF"
                     /locus_tag="ACG0BW_000418"
                     /gene_synonym="yqjH"
     CDS             complement(30692..31456)
                     /gene="nfeF"
                     /locus_tag="ACG0BW_000418"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFK7257432.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRAERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVMPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDHACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            31733..32356
                     /gene="nfeR"
                     /locus_tag="ACG0BW_000419"
                     /gene_synonym="yqjI"
     CDS             31733..32356
                     /gene="nfeR"
                     /locus_tag="ACG0BW_000419"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFK7257433.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(32461..33981)
                     /gene="aer"
                     /locus_tag="ACG0BW_000420"
     CDS             complement(32461..33981)
                     /gene="aer"
                     /locus_tag="ACG0BW_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFK7257434.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSASAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            34399..35778
                     /gene="ygjG"
                     /locus_tag="ACG0BW_000421"
     CDS             34399..35778
                     /gene="ygjG"
                     /locus_tag="ACG0BW_000421"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFK7257435.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALSECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLEGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(35820..36152)
                     /gene="ygjH"
                     /locus_tag="ACG0BW_000422"
     CDS             complement(35820..36152)
                     /gene="ygjH"
                     /locus_tag="ACG0BW_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFK7257436.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMEKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            36371..37354
                     /gene="ebgR"
                     /locus_tag="ACG0BW_000423"
     CDS             36371..37354
                     /gene="ebgR"
                     /locus_tag="ACG0BW_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFK7257437.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLLYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            37538..40630
                     /gene="ebgA"
                     /locus_tag="ACG0BW_000424"
     CDS             37538..40630
                     /gene="ebgA"
                     /locus_tag="ACG0BW_000424"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFK7257438.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVATTLEYSLFDGERVVHSSSIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNILEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            40627..41076
                     /gene="ebgC"
                     /locus_tag="ACG0BW_000425"
     CDS             40627..41076
                     /gene="ebgC"
                     /locus_tag="ACG0BW_000425"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFK7257439.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            41139..42572
                     /gene="ygjI"
                     /locus_tag="ACG0BW_000426"
     CDS             41139..42572
                     /gene="ygjI"
                     /locus_tag="ACG0BW_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFK7257440.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            42706..43776
                     /gene="ygjJ"
                     /locus_tag="ACG0BW_000427"
     CDS             42706..43776
                     /gene="ygjJ"
                     /locus_tag="ACG0BW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFK7257441.1"
                     /translation="MKLITAPCRALLALPFCYAFSAVAEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            43793..46144
                     /gene="ygjK"
                     /locus_tag="ACG0BW_000428"
     CDS             43793..46144
                     /gene="ygjK"
                     /locus_tag="ACG0BW_000428"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFK7257442.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLPNGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            46315..48333
                     /gene="fadH"
                     /locus_tag="ACG0BW_000429"
     CDS             46315..48333
                     /gene="fadH"
                     /locus_tag="ACG0BW_000429"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFK7257443.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDGGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(48378..48794)
                     /gene="higA"
                     /locus_tag="ACG0BW_000430"
     CDS             complement(48378..48794)
                     /gene="higA"
                     /locus_tag="ACG0BW_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFK7257444.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(48791..49105)
                     /gene="higB"
                     /locus_tag="ACG0BW_000431"
     CDS             complement(48791..49105)
                     /gene="higB"
                     /locus_tag="ACG0BW_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFK7257445.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(49390..50526)
                     /gene="rlmG"
                     /locus_tag="ACG0BW_000432"
     CDS             complement(49390..50526)
                     /gene="rlmG"
                     /locus_tag="ACG0BW_000432"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFK7257446.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGTKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            50611..51114
                     /gene="ygjP"
                     /locus_tag="ACG0BW_000433"
     CDS             50611..51114
                     /gene="ygjP"
                     /locus_tag="ACG0BW_000433"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFK7257447.1"
                     /translation="MSNLTYLQGYPEHLLSQVRTLINEQRLGDVLAKRYPGTHDYSTD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            51191..51874
                     /gene="ygjQ"
                     /locus_tag="ACG0BW_000434"
     CDS             51191..51874
                     /gene="ygjQ"
                     /locus_tag="ACG0BW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFK7257448.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            51953..52939
                     /gene="ygjR"
                     /locus_tag="ACG0BW_000435"
     CDS             51953..52939
                     /gene="ygjR"
                     /locus_tag="ACG0BW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFK7257449.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            53222..54187
                     /gene="alx"
                     /locus_tag="ACG0BW_000436"
     CDS             53222..54187
                     /gene="alx"
                     /locus_tag="ACG0BW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFK7257450.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHGMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            54803..56047
                     /gene="sstT"
                     /locus_tag="ACG0BW_000437"
     CDS             54803..56047
                     /gene="sstT"
                     /locus_tag="ACG0BW_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFK7257451.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(56052..56603)
                     /gene="ygjV"
                     /locus_tag="ACG0BW_000438"
     CDS             complement(56052..56603)
                     /gene="ygjV"
                     /locus_tag="ACG0BW_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFK7257452.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(56686..58173)
                     /gene="uxaA"
                     /locus_tag="ACG0BW_000439"
     CDS             complement(56686..58173)
                     /gene="uxaA"
                     /locus_tag="ACG0BW_000439"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFK7257453.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVTAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(58188..59600)
                     /gene="uxaC"
                     /locus_tag="ACG0BW_000440"
     CDS             complement(58188..59600)
                     /gene="uxaC"
                     /locus_tag="ACG0BW_000440"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFK7257454.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDRILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            60083..61381
                     /gene="exuT"
                     /locus_tag="ACG0BW_000441"
     CDS             60083..61381
                     /gene="exuT"
                     /locus_tag="ACG0BW_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFK7257455.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            61511..62287
                     /gene="exuR"
                     /locus_tag="ACG0BW_000442"
     CDS             61511..62287
                     /gene="exuR"
                     /locus_tag="ACG0BW_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFK7257456.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            62527..62610
                     /gene="yqiM"
                     /locus_tag="ACG0BW_000443"
     CDS             62527..62610
                     /gene="yqiM"
                     /locus_tag="ACG0BW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFK7257457.1"
                     /translation="MLMYQTRKTYQNSNNIAVVHLLKPAWR"
     gene            62632..63294
                     /gene="yqjA"
                     /locus_tag="ACG0BW_000444"
     CDS             62632..63294
                     /gene="yqjA"
                     /locus_tag="ACG0BW_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFK7257458.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            63298..63681
                     /gene="mzrA"
                     /locus_tag="ACG0BW_000445"
     CDS             63298..63681
                     /gene="mzrA"
                     /locus_tag="ACG0BW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFK7257459.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            63828..64196
                     /gene="yqjC"
                     /locus_tag="ACG0BW_000446"
     CDS             63828..64196
                     /gene="yqjC"
                     /locus_tag="ACG0BW_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFK7257460.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLTEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            64234..64539
                     /gene="yqjD"
                     /locus_tag="ACG0BW_000447"
     CDS             64234..64539
                     /gene="yqjD"
                     /locus_tag="ACG0BW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFK7257461.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            64542..64946
                     /gene="yqjE"
                     /locus_tag="ACG0BW_000448"
     CDS             64542..64946
                     /gene="yqjE"
                     /locus_tag="ACG0BW_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFK7257462.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            64936..65235
                     /gene="yqjK"
                     /locus_tag="ACG0BW_000449"
     CDS             64936..65235
                     /gene="yqjK"
                     /locus_tag="ACG0BW_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFK7257463.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGLWSAWRLVKTTLKQQQLRG"
     gene            65422..65814
                     /gene="yqjF"
                     /locus_tag="ACG0BW_000450"
     CDS             65422..65814
                     /gene="yqjF"
                     /locus_tag="ACG0BW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFK7257464.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            65884..66870
                     /gene="yqjG"
                     /locus_tag="ACG0BW_000451"
     CDS             65884..66870
                     /gene="yqjG"
                     /locus_tag="ACG0BW_000451"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFK7257465.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPSALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVVKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            67162..67527
                     /gene="yhaH"
                     /locus_tag="ACG0BW_000452"
     CDS             67162..67527
                     /gene="yhaH"
                     /locus_tag="ACG0BW_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFK7257466.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT        16260 a        17798 c        17316 g        16196 t
ORIGIN      
        1 gggtgatggc agcaggtttt aaacccgcac cacctgccgg gcaactgggt gcagtcattg
       61 tcgatcccta cggcaatgca ccactgaccg ctttggttga cttagatagc catgtcattt
      121 ctgatgtcaa agttaccgtc catgggaagg gcgaaaaagg cgtagaaatc agctatcccg
      181 tgggtcagga atcactaaaa acttatgatg gtgtaccgat ttttggtctt tatcagaaat
      241 ttgctaacaa agtgaccgtt gagtggaaag aaaacggcaa ggtcatgaaa gatgattatg
      301 tggtgcacac ttcggccatc gtcaataatt acatggataa ccgctctatc tccgatttac
      361 aacagaccaa agttattaaa gtcgcaccgg gttttgaaga tcgcctctat ctggttaata
      421 cccacacctt taccgcccaa ggttccgatc tccactggca tggtgagaaa gataaaaatg
      481 ccggtatcct tgatgcgggt ccggcaaccg gcgcactacc ttttgatatc gcgcccttta
      541 ctttcattgt cgataccgaa ggtgaatacc gctggtggct ggatcaagac actttctacg
      601 atggtcgtga ccgcgatatc aacaagcgtg gttatctgat gggtatccgc gaaacaccac
      661 gcggcacctt taccgctgta caaggtcagc actggtacga gttcgacatg atggggcagg
      721 tgctcgaaga tcacaaacta ccgcgcggat ttgctgacgc cactcatgag tccattgaga
      781 cgcctaatgg cacagtattg ttgcgcgtag gtaagagcaa ttaccgtcgc gatgacggcg
      841 tacacgtcac caccattcgt gaccatatcc tcgaagtcga taaatctggt cgcgttgtcg
      901 atgtatggga tctgaccaag atcctcgatc cgaaacgcga tgcactgctc ggcgcgctgg
      961 atgcaggtgc agtttgcgtt aacgttgacc ttgcccatgc aggacaacag gcaaaactgg
     1021 aaccggatac accgttcggc gacgcgctgg gtgtagggcc aggtcgtaac tgggcgcacg
     1081 ttaactcaat cgcctatgac gcaaaagatg actcaatcat cctctcctct cgccaccagg
     1141 gtgttgtgaa aattggtcgt gataagcaag tgaaatggat ccttgcgccc tctaaaggtt
     1201 gggaaaaacc gctggccagc aagctgctga aaccggttga tgctaacggt aagccaatta
     1261 cctgtaacga aaatggcctg tgcgaaaact cagacttcga ctttacctac acccagcata
     1321 ccgcctggat ttccagcaaa ggaacgctca ccatttttga taatggcgat gggcgtcatc
     1381 tggaacaacc tgccttacca accatgaaat actcccgctt tgtggaatat aagattgatg
     1441 agaagaaagg caccgttcag caagtgtggg aatacggtaa agaacgtggc tacgatttct
     1501 atagcccgat tacctccatc attgaatatc aagccgaccg taacaccatg tttggcttcg
     1561 gtggttctat tcatttgttc gatgtcgggc agccaaccgt cggtaagttg aacgaaatcg
     1621 attacaaaac caaagaagtg aaagtggaaa tcgacgtgct gtcagataaa cccaatcaga
     1681 ctcactatcg tgcactgtta gtccgtccac aacagatgtt caaataatta agaagggaat
     1741 aaaaatgtcg aaattaggga tctcatcact atttaaaacc atactgctta ccgcagcttt
     1801 ggctgtttca tttaccgctt ctgcatttac cgaaggcacg gattacatgg ttctggaaaa
     1861 acctattccg aatgccgaca aaacgctgat taaggtattt agctacgcct gcccgttctg
     1921 ttacaagtac gacaaagcgg ttaccggtcc ggtatcggaa aaagtgaaag atatcgttgc
     1981 ttttactcca ttccatctgg aaacaaaagg tgaatacggc aaacaagcaa gcgaagtctt
     2041 tgcggtcttg atcaataaag ataaagcagc aggtatttca ttatttgatg ctaactctca
     2101 gtttaagaaa gccaagtttg cttattacgc ggcttatcat gacaaaaaag aacgctggtc
     2161 tgatggtaaa gatccggctg cctttatcaa aaccggactg gacgccgcag gtatgagtca
     2221 ggccgatttt gaagccgcgc tgaaagagcc tgcagttcag gaaacgctgg aaaaatggaa
     2281 agcctcttat gaagttgcaa aaatccaggg cgttccggcg tatgtcgtca atggtaaata
     2341 cctgatctac accaagagta ttaaatctat tgactcaatg gcagacctta tccgcgagtt
     2401 agcaagcaaa taacagggaa acaggctatg ggtattaagg gaatgtggaa agaccttcgc
     2461 acttcaccgg tggacacgct ggtaagatgg caagaacaac gttttttgtg gttgttaatg
     2521 gccattgcga tgggcgcgtt aattatcctg gcgcactctt tcttccagat ctatctctac
     2581 atggcaccct gtgagcaatg tgtatacatt cgctacgcca tgtttgtgat ggttattgga
     2641 ggattggttg cagcgatcaa cccaaagaac atcatcctaa agctaattgg ctgcgtgatg
     2701 gcattttacg gcagcatttt gggactgaag ttttcgctca aactgaacga tatccaccat
     2761 gcagtacata atccggatcc cgattcactg tttggcgtac agggttgctc taccgatcca
     2821 acttttccct ttaacctgcc gctggcacag tgggcaccaa attggttcaa gcctaccggg
     2881 gattgtggct atgacgcacc aatcgttcct gatggcgtca cactgagcag cacgcaacag
     2941 tggtttgtgg aaatgtatca gcaatctgaa ggctggtatc tgctaccacc atggcatttt
     3001 atgaatatgg ctcaggcttg tatgctggct tttggtatgt gtctggtgct gctggtcatt
     3061 atgagtggcg catgggcgct taaaatcatc agaggctaac tcagcaacac ccgaagccag
     3121 tcgttctggc ttcgggttct tttattaacc tatctgcacc gacagcatac ttaaactgcc
     3181 catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccacg cacctaagac
     3241 atacaacaac ggcagatagt gctctggcgt tggattcgat aacgcgccac cttcatggtc
     3301 gagataattc accagaggat gctgctctac cggcccttgc cacgtcagat tcgctttcac
     3361 atactcatta aacgacatcg cccacggata cggtgaacta tcaccgtgcc acttcaccgt
     3421 acgcaggtta tgcaccacgt taccgctggc gaccaacatt attccttcat cccgcagcgc
     3481 tgccagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa
     3541 ctgcaccatc gggatatccg cttgcggata catcttaatc agcacgcccc acgagccgtg
     3601 gtcaaagccc caggcttctt tatccagcgt caccgggacc ggcgctaaca gctcaaccag
     3661 atgctgtgcc agcgcaggcg aacccggagc aggataatgc gtgtcgtaca gcgcctgcgg
     3721 gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg
     3781 ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa
     3841 cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc
     3901 taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat
     3961 tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg
     4021 atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc
     4081 agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg
     4141 cgttctcggg caaaaaccct cgaaccgctt actggcgttt tcgctaggat ttgcggcggg
     4201 gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat
     4261 gtcgcctgtg ttgggttatg gaatgtttat cttcggtctg cttggctatt ttggcctgga
     4321 ccgcatgttg ccacatgcgc atccgcagga tttaatgcaa aaatcggtgc agccgttgcc
     4381 aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc
     4441 ggaagggatt gccacctttg tcactgcgag cagcaacctg gagctgggct ttggcatcgc
     4501 actggccgtt gcgttacaca atattcctga aggtctggcg gttgcaggcc cggtttacgc
     4561 ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg atttctggac tggcagaaat
     4621 tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc
     4681 ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc
     4741 gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc
     4801 agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca
     4861 gaagttgtcc agcgcatgga caacttctgc cagcttcacg aaagccttat tctacctttt
     4921 cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc
     4981 tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca
     5041 gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta atatttaaca
     5101 atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt catatggtcg
     5161 cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc tgatattaat
     5221 gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg aaaaataaaa
     5281 accgaagcct gctacaactt cggtttcttg attaaggcag taaattaagc agcggttttc
     5341 agctggcttt acgctcatgt gcctgacgat aagccaccag gtcttcaata gtcacgagcg
     5401 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
     5461 cattagtcag ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcagatcaa
     5521 tagtcgcttc agtatggccg ccacgcgtca gtacaccacc agcctgagcg cgaagtggga
     5581 aaacgtggcc cgggcgattc agatcagaag gttttgcgtc atcggcaatc gctgcgcgaa
     5641 cggtcgtaat acggtcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
     5701 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa
     5761 gttgtttacg gcgatcttca gtaatgcaca ggcaaacaat accgctaccg tggcgaatgg
     5821 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
     5881 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
     5941 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
     6001 aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc
     6061 caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct
     6121 aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct
     6181 cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat
     6241 aagcggattc actataacgc taatgattag cgccagcaac gcatagcttc acataattct
     6301 ggtttatgac ttacccttat cgcactacaa tggcactcaa cacctatcag tacagggaaa
     6361 ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc
     6421 ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag
     6481 cgcaactgac gcgtctcgat ctggtgagcc gcgaagaatt cgacgtacag acgcaagtcc
     6541 tgttacggac tcgtgagaaa ctggcattac tggagcagcg catcagtgaa ctggaagcgc
     6601 gcaataattc ggtagcagat cttcaatctc ctccggctat cccgcctatt gataaagcag
     6661 aataaagcaa cacaaaaacg ggccagacgg cccgtaatat cagtgttcta attcaagttc
     6721 tctggtttgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
     6781 ttcttcatcc atgttaccag taacggccag aatatcgacc gggcgacctt ctgcgtgcat
     6841 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
     6901 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
     6961 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
     7021 actttttagt gaaaaataaa aaaccatatt aaaagacttt ctcttccaga acttctctaa
     7081 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcctaaa gtaaatattg
     7141 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata acacccctta
     7201 tcttttcgct atttcctttg tactgttgat cggcctttta gaaatttttg cgcttatctg
     7261 cggtcatatg ctctccggtg ctcttgatgc acatcttgac cattatgatt ccataaccac
     7321 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
     7381 tctctgctta ctggctggtt tttttggtct tattggcatc ctgttgcagc atacctgcat
     7441 catggtctgg caatcaccac tctcgaacct gttcgtggtt cctgtcagtt tactgtttac
     7501 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
     7561 ttcagctatt acagaagaag aatatattgg tagcatggcg ttaattaccg gacatcaagc
     7621 gacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     7681 actactggag cctgaagaag gaaaaatttt cactaaagga gacaaagtgt taattatttg
     7741 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccccaaa tattgtaatt
     7801 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     7861 tttaccgcaa ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     7921 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     7981 gtaatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     8041 aataccctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     8101 atgcgcgtcg atgtcgtggt cgctttcttt gtgcgggtaa aaccttcagt tgaagggatt
     8161 gccaccgccg cccagacgct gggacaacgc accctgtcgc ctgaagactt acgtatgttg
     8221 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     8281 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa ataccgtagc tgaagatctg
     8341 tcgaaaaacg gcctggaact ggaaagcgtt tcacttacca actttaacca gacgtcgaaa
     8401 gaacatttca atcctaacaa cgcctttgac gccgaaggtt taaccaagct gacgcaggag
     8461 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     8521 gagaaaaacc gtgatgcgct ttcgcgcaag ctggagattg aacaacaaga agcgtttatg
     8581 acgcttgagc aggagcagca ggttaaaacc cgtaccgctg agcagaatgc gaaaattgcg
     8641 gcttttgagg ccgagcgtcg tcgggaagca gagcagacgc gaattctggc tgaacgacag
     8701 attcaggaaa cggaaatcga ccgcgaacag gccgtccgtt ccagaaaagt tgaagctgaa
     8761 cgtgaagttc gcattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     8821 aaatcgatcg ctattgccgc caaatcggaa cagcagtcac aagcggaagc gcgtgccaac
     8881 cttgcacttg cagaagcggt aagcgcccag caaaacgttg aaaccactcg ccagaccgca
     8941 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggacgc ggaaaccaaa
     9001 gcagtcgaac tgaccgtccg ggcgaaagct gaaaaagaag ccgcagagat gcaggcggcg
     9061 gcaatcgttg agttagccga agctacgcgt aaaaagggtc tggcggaagc agaagcgcaa
     9121 cgtgcgctga acgacgctat caacgtactt tctgacgagc aaaccagcct taaattcaaa
     9181 ctggcgctat tacaatcatt acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     9241 attgacggca tcaagattat tcaggtcgat ggattaaacc gaggtggcgc tgcggggaat
     9301 gcggcatcag gcagcgttag tggcggaaac ctggcagaac aggcattgtc tgccgccctt
     9361 tcttaccgca cacaggcacc actgattgac tccttgctca atgaaatcgg tgtttctggc
     9421 ggctcgctgg cggcattgac ttcaccctta acatcaacaa ctcccgtcga agaaaaaaca
     9481 gagtaaaccc tcttctatga ataatccctg gcccaaaaag ccggggattt tttatatctg
     9541 cgttccgcta aaaggtgcaa atgatcaggc tgttgcagca ttttgcgtga ccgctcgggg
     9601 aagggaaaac tgcctctggg aaagcgttgc gcggggtctg gcgctcatca acaatcgggg
     9661 agtcagcagg gggctgaaac gggaaagccc ctcccgaaga aggggcctta tagaaggaaa
     9721 gggttatgat gaagctcgtc atcatactga ttgtgttgtt actagtaagt ttccaggctt
     9781 attaacaacc aattcagagg ggggagaaat cctcccttac ccttgttcct ttactttagg
     9841 ttgaaaaaaa atcagcgtca agaaggtttc tctatcccac atccctggcc caaaaagccg
     9901 gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg atcaggctgt tgcagcattt
     9961 tgcgtgacgg ctcggggaag gcaaaactgc atcagggaaa gcattgcgcg gggtctggcg
    10021 ctcatcaaca atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaagaagg
    10081 ggccttgtat aaggaaaggg ttatgatgaa gctggtcatc atactgattg tgttgttact
    10141 cgtaagtttc gcagcttatt aacagccaat cagagggggg agaaatcctc cctcaccctt
    10201 gttcctttac tttaggttga aaaaaaaaca gcgtcaatag gcctgccagg ttcgaagcga
    10261 gatctgtgaa ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt
    10321 ggtcgtcgag caaccgtctt caaagttgag caccaacact tcgccaccgt tggcccagac
    10381 ttctttactc ccggcaatct cttctggttt atagtcgccg cctttcacca gcaggtctgg
    10441 caagatcccg gcgatcaggc gctgcggcgt gtcttcttca aacgacacca cccagtcgac
    10501 cgcttccagt gcacccagca caatcatacg ctgctcgaga gggttaacgg ggcgggaatc
    10561 ccctttcagc cgtttggtgg aagcatcgct gttgacggcg acaatcaaac ggtcacccag
    10621 tttacgggca tttgccagat aagagacgtg cccggcgtgc aggatgtcaa agacgccatt
    10681 ggtcattacc accttttcgc cgcgtttacg cgccgccgcc acagcaagct tcagttcctc
    10741 ttcggtcatc acaccaaagc cggtatctgc acgtccacgt actgcgtttt ccagctcgat
    10801 cggcgaaacc gtggatgtcc ccagtttgcc gaccactacg cctgccgccg cattagcaaa
    10861 gaagcaggct tcttccagcg aattacccgc cgccagcgtt gccgccagga caccaatcac
    10921 cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc tgagttggca tatgcagcgg
    10981 cgttttgccc ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga
    11041 gagttcgtaa tcggcaatca gtttcatgcc gcgttcaaca atctcttctt cggtcttaca
    11101 tttaccgaca acagcttcaa attccgagag attcggcgtt aacagcgtag cgccgcggta
    11161 gcgctcaaaa tcggtgcctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag
    11221 ttggatcatc tgctgcacgc ttgccagcgc acctttcgcg tagtcagaga gcaccagcgc
    11281 gccaatcgaa ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caaccccttc
    11341 gaaaccttct tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt
    11401 ggtgatggtc ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag
    11461 agatttactc agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt
    11521 agcaccgaga gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat
    11581 ggtattcact ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta
    11641 ccagtaacga tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg
    11701 cagcgttact ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata
    11761 cttcattctt ccaccagcca cttctgccag cttgcccgca ccagttcacg ctctgcggtg
    11821 aagcaatcct ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg
    11881 cgcagcgtcg tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt
    11941 tgcgccagta gttccagaat gcgcacgttg tctgaccagc gcgttaactt cggtttttca
    12001 tgagcgtagc gcaacaccag atattgagta ataaattcga tatcggtaat tccgccttca
    12061 tcggctttga tatcaaagcg atcgcgatgt ttattgccga gatgggcacg cattttctcg
    12121 cgcatttccc gcacttctgt ttgcagggtt ttgccttcgc gcggcagcgt cataatctcc
    12181 cggcgtactg cgtcaaagtg cgcggtgagc tgcggatcgc cgtacactac acgcgcacgc
    12241 accagcgcct gatgttccca cgtccaggcc tcgtttttct gataatcggc aaacgcttct
    12301 gcggaggtaa ccagcattcc cgccgccccg gacggacgca gtctagcatc cacttcatac
    12361 aaaatgccgg aagaggttcg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga
    12421 taaaactgtc gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca
    12481 tggaggaaga tcaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg
    12541 tagccgacca ccgcaaaacc acgcccttcg cgttcgttca ggtgatttgg cttaccgtag
    12601 cggacaacca tttgaaccca cgcctgctga acgacggcat ctatcatagc ctccgccagc
    12661 caggttaagt gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca
    12721 atacgtaaca gttgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca
    12781 tcttccggca cgcgcagcaa atactggcgc agttcatcac ggtaggcatc cgtcgccgtc
    12841 ggctggtaga gagtgtttgg atcgagcagt tcgtccagca ataatggata acgcgccagt
    12901 tggctggcaa tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcgggg
    12961 aattcactga gcaattctaa ataggtggtg cgggtaacaa tccccaccag caaggcggta
    13021 atgcgcgaca gcgtgacagc agcgtcttca cgcgcacata catcacttag cagatgcggc
    13081 atcagatggt cgagcacctg acgcccgcgc ggcccaatag tgcgcttatc cagctctttg
    13141 cggaaatcag caatcagcgt cagcacctgt ttgcgatcat cttcgctaag atgcgccagc
    13201 actggcgtag tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac
    13261 agcgactctt cctgagtttc gctttcgtcg tcgccaatca attcattaaa cacccggcgc
    13321 acattggtca tatgtacggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc
    13381 gcccacgcca gccgcgcacg gttaagctca tcagaaggaa gcgtctgggt ttgttcgtcg
    13441 ttaatacttt gcagcaggtt ttccagacgc cgcaggaaca gatacgccac tcgcaattgt
    13501 tcagcatcgt tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa
    13561 gagcgcgatt gcagcgacgg ttcgcgcccg ccgcgaatga gctggaatac ctggacgata
    13621 aattcaattt cgcgaatgcc acccgcgcca agtttgatgt tgtcggtcaa accacgtcga
    13681 cgcacttcgc gggcaatcat ccctttcata ttgcgcagcg actggatgac gctgaaatcg
    13741 atgtaacggc ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct
    13801 tcgctatcgc ccataatccg cgccttgacc atcgcgtaac gctcccagtc gcgcccctgc
    13861 tcctggtaat aatcttccag cgccgcaaag ctcagcacca gcgggccact ttcgccaaac
    13921 ggacgcagcc gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact
    13981 ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg
    14041 ccctgcgtac aaccatgttc tggccaggca aaaatcagat cgatatctga ggagaaattc
    14101 agttccccac cgcccagctt acccatgcct aaaatcagca gcggttgcgc ttcgccctgc
    14161 gcattgcacg gcgttcccca ctcgcggcag caagcgtcat acagccagtc acgcgccgca
    14221 acaatcagcg tctccgccag atggctgagc tgctgcaata tgctctcttc agtaaccagt
    14281 gccagcgttt gcgcccaggc gatgcgcacc ataatgcgcc gccggaatag ccgcagttca
    14341 cgcattaacc cggcttcgtc actcacatta ctgagcgcct cctgcaacca ggcagcgtaa
    14401 tgctgccatt cgtcggcctg cggcggctgg ctttgcagtt ccgtcagcca ctctggatgc
    14461 gcaattacgc tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcgcta
    14521 agtgattcct cggctaaagg ctctggcagc cgctcaacaa cggtctgcca gtactgctgt
    14581 aacggtgaag agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct
    14641 gtgcaaccag aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg
    14701 ttgcccggtc gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac
    14761 gagaggatcg tagtaacccg ccagcaacag tattgagtca atatcacgcg tcaggcgtgg
    14821 caactggtca cggtagcgat cccccaacgg ctggcagaaa acgcttttca gttcagcggc
    14881 atggcgggaa agatggatat cggcaaagcg tttgaaggaa tcgctcattt tactctgcgc
    14941 tttggcatct aaaaacggct gccaggcttt gcttaccagc cattcggtca atgccagctt
    15001 cgccattgcc gtttcggtag agtagaccgc cgtcacggca gaaaccgctg aagcgatggt
    15061 cgcttcgcat tgagtcaata aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc
    15121 accgaacaac atcagcgtat gacggaccag gccaatggct gccagcacct gctctttcgc
    15181 cgcatcattg ccgcgtaccc atagttcttc atgatactgc cattgcgcta acgccagctc
    15241 gagcgccgct tccagcccct gctcgacatc cgcttttgcc gcaacatgta aaatggtggt
    15301 cggtttaatt tcacgcgcgg gattaccctg cgccaaatga tagccacgcg ccgctttgct
    15361 caggctgccc tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc
    15421 gcgcgtgtcg ccgctaagca gttccagttc cagctcgcag ataggttcag caaattcacc
    15481 cgctttcact tccccctggt cgagggcgat ttcaatttgg ctaccatcaa cctctaccag
    15541 ccacttttcg cgataaaaat cggtgctgaa cagcggttgt acgcgggaag cgagatcggc
    15601 aggcaattcg ccgttcggcc agacttccgt cggtaactgc gccaggtcga gcgtcggttc
    15661 gctcaacgac acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac
    15721 tttcatggtc atctcatagc gaccgttttc gccacgaata cgcaagccca tgtcgtgccc
    15781 acgcagccag ttatccggcg tttcgtagta aatattcaac aactgcacgg ggtcatggtg
    15841 ctcgccgccc agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat
    15901 aaactttaat tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga
    15961 cgatgaacgg cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt
    16021 gccatgcaac gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca
    16081 gaggattccg attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc
    16141 cactttccat gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt
    16201 actcgcactt agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttccgacga
    16261 actgaatacc tgggtccgta gcggtccggg agaccattat cgcctcgtgg gcacggttaa
    16321 cgccggcgag gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa
    16381 agacagctct ggccgtaccg cctggatccc gttgaaacaa cttagcacag agccaagcct
    16441 gcgctcccgt gtgccagatc tggaaaatca ggtcaaaacc ctgactgata agctcaccaa
    16501 tatcgataac acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga
    16561 cagcgtgatc aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc
    16621 gcagaaaaag gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat
    16681 gcagtggttt atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact
    16741 gccgcacctg atcccaacgc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt
    16801 cgttgcactg tcttattatt aggaattgtg tatttgacga agggaaaatg gcgtgaagat
    16861 ttatctggtc ggtggtgctg ttcgggatgc attgttaggg ctaccggtca aagacagaga
    16921 ttgggtggtg gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg
    16981 ccgcgatttt cctgtttttc tgcatccgca aacccatgaa gagtatgcgc tggcacgaac
    17041 cgaacggaaa tccggttccg gttacaccgg ttttacctgc tatgccgcac cggatgtcac
    17101 gctggaagat gatcttaagc ggcgcgatct gaccattaat gcgctggccc aggacgataa
    17161 cggcgagatt atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca
    17221 tgtttccccc gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc
    17281 gcgttatgcc cacctcggtt ttcgtattgc cgatgaaact ctggcgctga tgcgcgagat
    17341 gacccacgcg ggtgaactgg aacacctgac gcctgaacgg gtgtggaaag agacggaaag
    17401 cgccctcact acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact
    17461 gcgcgtttta ttcccggaaa ttgacgccct gtttggcgtt ccggcccctg ccaagtggca
    17521 tccggaaatc gacaccggta ttcacacctt aatgacgctc tcaatggcgg cgatgctgag
    17581 tccgcaggtc gatgtccgtt tcgcaacgct atgccacgat ctcggtaaag gactgacgcc
    17641 accagaactt tggccgcgtc atcatggtca tggcccggcg ggtgttaagt tagtggaaca
    17701 actctgtcag cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga
    17761 gtttcacgat ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattgtt
    17821 tgattccatt gacgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagtga
    17881 ggctgatgtg cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgctggtt
    17941 gcgcgaagcc tgggaagtgg cacagtcagt gccgacaaaa gccgtcgttg aagcgggatt
    18001 taaaggtgtg gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg
    18061 gaaggaacag cgttgcccaa aacctgagtg agtgttggat attttgccgg atgcggcgta
    18121 aacgccttat ccggctacat gtcaacgaca gttgtaggcc tgataagacg cgcgagcgtc
    18181 gcatcaggcg ttgattgccg gatgcggcgt gaacgcctta tccggcctac gcaatcgttg
    18241 acaattccac gcatccgaca ctgttcttta cctcaaaaga acacgacata caccgcagcc
    18301 gccacaataa agcgataaat ggcgaacggt ataaacgaaa tgcgcttaat caattgcagg
    18361 aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc
    18421 gggatatcgc cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc
    18481 atcatcatcg gcaccgccag caggaacgaa aactcggaag cagcgtaacg gctcaccccc
    18541 atcagcatcc cacctgaaat ggtcgccccg gagcgagaga aacccggcca cagcgccaga
    18601 cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcgag acccggcgca
    18661 cgcggctctt tcggcttcag gcattcagcg gcaatcagca acagaccgcc aacgaccagc
    18721 gcatacatca catttattgg attaaacaac gacttaatcg tgtcgtggaa caacagcccc
    18781 aatactaccg ctgggatcat gccaagcaga atgtggatca gcgttaaacg acctttgctt
    18841 tctccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc
    18901 cagaacatca ctactaccgc cagaattgat cctaactgga tcacaacctc aaaggttttc
    18961 gccgtatcgc cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat
    19021 accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag
    19081 tgcatatcgc tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact
    19141 gcagtatgac caggttttaa ccgtttggtt taacagctgt aaaattaatt attttctttc
    19201 agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc
    19261 ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc
    19321 tcttccgcca cacgttccac cagcgcaaaa cgcgccccct cgacgtggct gactaccgtt
    19381 tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg
    19441 ttatcccacg ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg
    19501 taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc
    19561 tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat
    19621 tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat
    19681 gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg
    19741 tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg
    19801 tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt
    19861 gcctgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat
    19921 cgccgcctgc cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt
    19981 tgctaccgct tttggtgcca tcgcaccaat tggctgggat ctcaccggag taatggcggg
    20041 aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc
    20101 actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat
    20161 gctctcttgc ctgatcctgc tgcgtcacca tgacaacatc cagcgtctgt ggcgtcgtca
    20221 ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct
    20281 ggtggatcta cataacctga tagccttcgt cgggcttgcc cagccgttgc tggcaccatg
    20341 ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa
    20401 cagcccagat attggcgctt tgtgcatact ccggcaatac tcgcaccagt ttgccactct
    20461 ccagaaacgg caacacatcc cactcggaac gcaacataat ccctttgccc tccagcgccc
    20521 attgcaacac tatctcgccg ctattggagg aaagatgccc gcttaccttc accgattttt
    20581 tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca
    20641 gacaatcatg acgacttaat tcctgcaagg attgtggctg cggatatttt tgcagatact
    20701 caggtgcagc acataatatt cgtttatttt ttgtcaaaag atgcgcaata taataatcag
    20761 gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa tcaatttgcc
    20821 gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcatcagt tcggtaatag
    20881 ctggcgcaat atggctgcgc ccaaaaccaa aactacagcc aatacgaatc atcccctccg
    20941 ggcgcgtttt gatttgcgta acgtcatcga ccagtcgctg atattgggtg aggatttcca
    21001 gcgcatgctc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga gcggagcggt
    21061 tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg acaaatgcag
    21121 gcgtttgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg atttctacca
    21181 gtacctgcaa atctttggct aaaggccagc tattcagcat cgttaattat ccgcagttgt
    21241 gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca atattcattc
    21301 ttattttcgc gttgattcac gaactgtgaa ttacatattc acggcggcaa aaaatataaa
    21361 accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac ataaactgga
    21421 gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac ggagattgtc
    21481 gctaacttta ccgccatgat ttctacccga atgcccgatg acgtggtgga caaactaaaa
    21541 cagctaaagg atgccgaaac gtcgtcgatg gggaaaatca tctaccacac gatgttcgac
    21601 aacatgcaaa aagcgatcga cctgaatcgc ccagcctgtc aggacaccgg cgaaatcatg
    21661 tttttcgtta aagtcggttc ccgtttcccg ctgcttggcg agctgcaaag cattcttaag
    21721 caagccgtgg aagaagcaac cgtcaaagcg ccactgcgcc acaacgcggt ggaaattttt
    21781 gacgaagtga acaccggcaa aaataccggc agcggcgtac cgtgggtcac ctgggatatc
    21841 gtccccgacg gtgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgttg
    21901 cctggtcgtt cgaaagtgtt aatgccgtcg gaaggctacg aaggcgtggt gaaattcgtc
    21961 ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt aggcgtgggc
    22021 atcgccacct cggtggaaac cgccgctatg ctctcacgta aagccatttt gcgcccaatt
    22081 ggcagccgcc accccaatcc gaaagcggca gagctggagc tacgcttgga agaaggactc
    22141 aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat gggcgtgcat
    22201 atcgaatctg ccgctcgcca tccgtcaacc atcggcgttg ctgtttctac aggctgctgg
    22261 gcgcatcgtc gcggcacact gctggttcat gccgatctct cctttgaaaa tctgtctcac
    22321 acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag
    22381 atattcgcgt cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgtgacgttt
    22441 gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga
    22501 ttttccacgc tggccccatc gtgcgcaaaa acggcgacaa atgggagatg gtatccgtcg
    22561 gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgaa cagaccggcg
    22621 tgaaactggt ggtcggcaaa ggcggaatgg ggccgctgac cgaagaaggc tgccagaaat
    22681 tcaaggcgct acatgtgatt ttcccggcag gctgcgcagt actggcggca acccaggtgg
    22741 aagagattga agaagtgcac tggacagaac tcgggatgcc ggagtcactg tgggtctgcc
    22801 gggtcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac aacctgatag
    22861 ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atttgcgagc
    22921 acgtccacta catcaaataa ccctcccgga gaggctcacc ccctctcctt tttcgcaggc
    22981 ataacacgat gaaaccttcc actgaatggt ggcgatacct tgcgccgctg gcggtcatcg
    23041 ccattattgc tctacttccg cttcccgcag ggctggagag tcatacctgg ctctactttg
    23101 ctgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt gccgtggtgg
    23161 cgatggtggg tatctccatt atcgccattc tctctccctt gctgctgttc agcccggagc
    23221 agctcgccca gccaggtttt aagttcaccg ccaaatccct ctcctgggcg gtttctggtt
    23281 tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggtacg ggctatgaaa
    23341 aaaccgggct tggacgtcgc atcgcgctga ttctggtgaa aaagatgggg catcgtacgc
    23401 tgtttctcgg ctatgcagtg atgttctccg agctgatact tgcgcctgta acaccgtcca
    23461 actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgcca ccgctctatc
    23521 aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg tggatgggga
    23581 ttgttgccga ctgcgtgacc agcgccattt tcctgacggc gatggcacct aacttgctgt
    23641 taattggcct gatgaaaagc gcatctcacg ccacgctgag ctggggcgac tggttcctcg
    23701 ggatgttgcc gctcagcatt ttactggttc tgctggttcc ctggctggct tacgtgctgt
    23761 acccgccggt actgaagtct ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg
    23821 caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg
    23881 tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac agcgtggtgg
    23941 cgctgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat aaagccgcgt
    24001 ggaacgtttt cttctggctg gcctcgctta tcaccctcgc taccggactc aacaacaccg
    24061 gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat tcgccgacga
    24121 tggtgatggt ggcgttgatt gtggtctttt atctgctgcg ctacttcttt gccagcgcca
    24181 cggcgtatac ctccgctctc gcgccgatga tgattgccgc cgcgctggcg atgccggaaa
    24241 tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg agcattctta
    24301 cgccatacgc caccggaccc agtccgattt actacggtag tggttatctg ccaacggtgg
    24361 attactggcg actgggggca atttttgggc tgatattcct cgtgttgctg gtgattaccg
    24421 gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa gtgactctcc
    24481 ggcacctaag agttttacgc ggccggtaac tctgccagcg gccagcgcgg acgcacgcta
    24541 acgccaagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata ggcgatcatc
    24601 gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccacg gcgttttttc
    24661 atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccacc tgccattacc
    24721 agtcgcttaa agcccgtctg atccagcgct cgcttacact taatcatcaa cgtatcgacc
    24781 accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt gccgttatca
    24841 cgaatggtat ttgccgcgaa ggttttcaga ccagaaaagc tgaaatccag ccccggacgg
    24901 tcggtcatcg ggcgcgggaa gacaaagcgc ccggcagtac cctgagccgc cattttcgac
    24961 agtaatggcc cgccaggata atccagcccc agcagcttcg cggttttgtc aaacgcttcc
    25021 ccggcggcat catcaataga ctcgccgagc agctcatact gaccaatgcc agtgacgctg
    25081 attaactgcg tatgaccgcc ggaaaccagt agcgccacaa acggaaattc cggtgggtta
    25141 tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat cgccggaacg
    25201 tcccaggcaa acgccagaga acgccccacg gtcgcgccaa ccagcagcgc gccgactaat
    25261 ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc agactccttt
    25321 agcgccgcct ggatcaacgg tacggtttta cgcacgtgat cgcgggaggc cagttcaggc
    25381 acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt ggctaacaaa
    25441 cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc aatacccagt
    25501 acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca atgcgggttg
    25561 atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc cttataaaag
    25621 tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt aaaattccgc
    25681 accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga
    25741 ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct
    25801 tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg
    25861 aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga
    25921 aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag agcgttgctc
    25981 tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt
    26041 ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac gcgtattcat
    26101 taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg taaagctgaa
    26161 aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa ccccgtcatt
    26221 caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc acggcaacgc
    26281 gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg aagagctggc
    26341 agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc agatcgagcg
    26401 ccatcagcgg caaacgcttt atcagttgat ggacggtctg aatacgtttt accaacaatc
    26461 tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca
    26521 cgaggttatc gcccgctttg cgattggttt tgcgcccccc ggctgggaca acgtcctgaa
    26581 gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggga tgttggtcac
    26641 taacgatcag gggcgcagtt acgaccgttt ccgcgagcgg gtgatgttcc ccattcgtga
    26701 taaacgcggt cgggtgattg gttttggcgg acgcgtgctg ggcaacgata cccccaaata
    26761 cctgaactcg ccggaaacgg acattttcca taaaggccgc cagctttacg gtctttatga
    26821 agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct atatggacgt
    26881 ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta cgtcaaccac
    26941 cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct gttatgacgg
    27001 cgaccgtgca ggccgcgatg ccgcctggcg agcgctggaa acggcgctgc cttacatgac
    27061 agacggccgt cagctacgct ttatgttttt gcctgatggc gaagaccctg acacgctggt
    27121 acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc cactctccgc
    27181 atttctgttt aacagtctga tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg
    27241 tttgagtacg ctggcactgc cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata
    27301 tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc ttgaacgatt
    27361 aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac
    27421 catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc
    27481 gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag aactggtcaa
    27541 cacttgtctc tctcagccag gtctgaccac cgggcaactt ttagagcact atcgtggtac
    27601 aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag ataagaatat
    27661 tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc ttgaactgcg
    27721 ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag aacgcctgga
    27781 gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa gtgccgaaga
    27841 gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat aagtacgccc
    27901 tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc tgaaggacat
    27961 cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct tatggagcaa
    28021 aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg ctatctgacc
    28081 tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca gatcgaagac
    28141 atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc ggatgccgat
    28201 gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg
    28261 ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat gtacatgcgt
    28321 gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt
    28381 gaagacggga tcaaccaggt tcaatgctcc gttgctgaat atccggaagc gatcacctat
    28441 ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga tctgatcacc
    28501 ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca cgtcggttct
    28561 gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga tggcgatgac
    28621 gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgtgaaaa atttgcggaa
    28681 ctgcgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct
    28741 gccgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg cctggtgccg
    28801 aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa
    28861 gaacgtctga tcatgaagct ctgtgttgag cagtgcaaaa tgccgaagaa aaacttcatt
    28921 accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat tgcgatgaac
    28981 aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc cctgcagaaa
    29041 ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga tatcaaccgt
    29101 cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac
    29161 ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca gttccttgac
    29221 ctgattcagg aaggtaacat cggcctgatg aaagcggttg ataaattcga ataccgccgt
    29281 ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac ccgttctatc
    29341 gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat caacaaactc
    29401 aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc ggaagaactg
    29461 gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat cgccaaagag
    29521 ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg ggatttcatc
    29581 gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct gcgtgcggca
    29641 acgcacgacg tgctggctgg cctgaccgcg cgtgaagcga aagttctgcg tatgcgcttc
    29701 ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc
    29761 cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt
    29821 tctgaagtgc tgcgtagctt cctggacgat taatcggtag gccggatcag gcgttacgcc
    29881 gcacccggca ctaggccctc tgcacaaacg ccaccttttc ggtggcgttt tttatcgccc
    29941 acgcactacc agcgcctggt ccagctcgcg atacgcttca accagtttct ccagtgaaac
    30001 gcgacttaaa ccactgggat ttggcagcac ccaaatctgc gtcgaaccaa tggtgagcgt
    30061 ttgtttcccc cactgtgcac cgcgctggct gaatccctgt tcatatgctt gtttgcccag
    30121 aatcgccaac gcctgtggct gataatcttc tattttttca attagcttac gcccgcctgc
    30181 gtgcagctcc tgctttgaaa cttcattggc ttgcaccgtt ggacggtcta ccagtttggt
    30241 gacgccacaa cgataatcca gcagatgctg tgcctcctgc ggcttcaact gacggtcggt
    30301 aaacccggcc tgatatatca ccttccagaa gcgatttgcc ggatgagcaa agggaaaacc
    30361 agtcccggcg gatgaaagcc cagggttgat accgcaaaac acgacccgta accctggagc
    30421 caaaatatcc tcaaccatat ttactccttt ctaacatcac caggaaaagt ataacggatt
    30481 gaaaatacat tgtttataaa aacagcaggc gcgcggtaat ggctggattg cgacacggag
    30541 ttactttata atccgctacc atggcccctt agctcagtgg ttagagcagg cgactcataa
    30601 tcgcttggtc gctggttcaa gtccagcagg ggccaccaga tatagcaaag gctgacgaga
    30661 aatcgtcagc ctttttcttt ttatatatca gttactttgc gtgccagtaa gccgctgcac
    30721 gtacccgctg tggatcatac tgttccgctt caaagcggcg gcttaaattc ttaacgactt
    30781 tgccttcgcc ggttatccag atgaagtaat catcggcagg gatttgcatc tgcgccagac
    30841 gcgcatctac cgcctgctca tcatgtgcca gccattcgat attaaaacca tcaaggtgcg
    30901 cgagataatc ctgacaggcg tgatcccgca cgctaaccag cgcgctaact tgcggtttaa
    30961 cagcaagttt gctcaacgtt tccaggcggc ggcgcagtgc gggcattccg gattcatcgc
    31021 agacatacag ctgatacgcg taatcctctg gcatcaccag cgaaccgcgc ggacctgcca
    31081 ccgtgagttt atcgcccggt tgcgcctgca ttgcccagct gctggcgacg ccaccttcgt
    31141 gaataaagaa atcaatcgcc agttcatggc gtagttcgtc atacagcggc gtatagtcac
    31201 gcgacggtgg gcgtggtcct tccggccaga cgatgccctc ttccgttacc gttggcggca
    31261 caaagtgagc atcagattga ggaaagaaga gtttgctgtg atcgtcaaag ccgcgcgatg
    31321 taaaaccgtc cagcgcctcg ccgccaagga caatgcgctg aaaaccggcg ctaatacgct
    31381 cagcgcgtag cacagtcagt tcacggaagc gcagatcatt gcgaacgcgc tgcgggtagc
    31441 ggggggagtt attcattgtt atcgccttcg tgatagtaat cagatatatc taaataaatt
    31501 tcgcaaatga taatgattgt taatcatgat aaatgcaagc gatttgtaga actgatatat
    31561 ctgaagtatg agaagacatt cagcgtcgta gcaaggctgt tggatgcggc gtgaacgaat
    31621 tatccggcct ggatgtgagg tgtattcacg tttaaaaatc atttttatac ttgcaaaagc
    31681 gctcatatca gatataaatt agatatatct aattaagcaa aaggaggctg atatgagcca
    31741 tcatcacgaa gggtgttgta aacatgaagg ccagccacgc catgaaggct gctgcaaagg
    31801 tgagaagtca gaacatgagc actgcggaca cggtcaccag catgaacacg gtcaatgctg
    31861 cggtggtcgc cacggtcgcg gcggcggtcg tcggcagcgt ttctttggtc acggtgaatt
    31921 gcgtctggtg attctggata ttctctcgcg cgatgacagc cacggttacg aattgattaa
    31981 agcgattgag aatctaaccc aggggaatta caccccaagc ccgggtgtca tctacccgac
    32041 gctggatttt ctgcaggagc agtcgctgat taccatccgc gaagaggaag gtggtaagaa
    32101 gcagattgcg ttgaccgaac agggcgcgca gtggctggaa gaaaaccgcg aacaggtgga
    32161 gatgatcgaa gaacgcatca aagcgcgttg cgttggtgct gcactccgcc agaacccgca
    32221 aatgaagcgg gcgctggata attttaaagc ggtgctggat ttacgcgtca accagagcga
    32281 tatcactgat gcacaaataa aaaagatcat tgcggtgatc gaccgcgccg cttttgatat
    32341 tacgcaactg gattaatcac cgattgccgg atgtggcgtg ccctaccccc ccgtaggcct
    32401 gataagatgc gccgtatcgc atcaggcatt gtgcgccaac cgccggatgc ggcatatcga
    32461 ttaatgcagc accgtcaccg cgtcttccag tcggctggcg cggtgtttta ccatcgccga
    32521 cacctgcgca ctctcttcca ccagctcggc atttttctgg gtgatgaggt taagctcatc
    32581 cactgcacgg gtcaggctgg aaagcccatc ggcctgttcc agcgttgaat ggctaatctg
    32641 ggcgatcaac tgggtgacgt ttttcacctg tgccacaata tcttccatcg tccgtccggc
    32701 tgcgtgtacc tgctgcgaac cggattgcac cttatcagca ctggcatcga tcagcttgcg
    32761 aatatcgttg gcggcactgg cgctgcggct ggctaaatga cgcacttccc ctgccaccac
    32821 tgcaaaacct ttgccttgtt cgccggctcg tgccgcttcc accgccgcat tcagggccag
    32881 aatattggtc tgaaacgcaa tatcgttaat cagcgaagta atggtgccaa tgcgctgggt
    32941 actgtcggcg atatcgtcca ttgtcttgat caccgtggtc atcgcctccc cgccctgcac
    33001 tgccgcatta ctggcggtga tagaaagttt atcggcagcc gacgccgtgg cagagttctg
    33061 tttcaccgac gccgccattt ggttcatggt cgccaccgtt tgctgaacgt tatcaactgt
    33121 ctgctgggta tgttcgttca gttcatcggt acctttcgcc agcgtctcac tgccgtttct
    33181 gacactggac acctggcttg agacatcgtt aattagccaa cggcacatca ggccaagctg
    33241 ccctaccgcg cgtaatgtca gccccagctc atcgctgcga ttcagatgct caacactatt
    33301 acgctctccg gtcgccacct tcagtgcctg acgggcgaca ttttctatcg ggcggacaat
    33361 ctgccattca aaacaggcgc ttgccaacaa taccactaac gcacagagga tatacgtcac
    33421 caccggggca gcaacaaacc aaagcatgac cgccagcaag ataaacatca gggtcatcac
    33481 gccacgcgcc cgccagcgaa gcggtaatga aggcagtttc cccagccagc ctttacgcac
    33541 caccaggcct ttatgaatac gcttactggt acgtccggcg ttcaacgctt tgtacagcgg
    33601 ctccaccgcc gcgatctctt catccgtcgc ccgggtacga atcgacatat agccactgat
    33661 ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc caataatggt cgccattttt
    33721 gcggcgattt ttcacgatgc cgctccaggg ctcccctttt ttcagggtga accacatatc
    33781 cgcaaacgcc gcttttggca tatccgggtg acgcaccatg ttgtgcggct gcccttgtaa
    33841 ctcttgcaag gtatagccgc tcacctgcac aaaagtgtca ttagcatgag tgatatagct
    33901 ttgcagatcg gtagtggaca tcagagtggt atcgtccgcc agcggggtat tttgctgggt
    33961 gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt tatatggttg ttaacttctt
    34021 gtcagagttt atgtcggccc cgctgcggtt atctttaact gattaatttg atttagatcg
    34081 caatttgcga ttaaagcata aatctaatcc cttgatttaa aatactttca ctctgttact
    34141 atacgaaaac gttaattatc ttgcccaaaa atcaggcagt tgttgccctg aaagtgtgca
    34201 tttgcgcagc aatcgccaaa tccatacccg acaaaaaccg tgcaaaataa caacagatgt
    34261 taacaaatag cattaaatat tgcgcaaatg ataaccacat ttgtgtttat cccgattttc
    34321 gcgatcgcag ccggagtggc gcaatccctg caatacttaa atcggtatca tgtgatacgc
    34381 gagcctccgg agcatatttt gaacaggtta ccttcgagcg catcggcttt agcgtgcagc
    34441 gcccacgccc tgaatctcat tgagaagcga acgctggatc atgaggagat gaaagcactt
    34501 aaccgagagg tgattgaata cttcaaagag catgtcaatc cggggttttt agagtatcgc
    34561 aaatctgtta ccgccggcgg ggattacgga gccgtagagt ggcaagcggg aggtttaaat
    34621 acgcttgtcg acacccaggg acaggagttt atcgactgcc ttggaggttt tggaattttc
    34681 aacgtggggc accgtaatcc agttgtggtt tccgccgtac agaatcaact tgcgaaacaa
    34741 ccgctgcaca gccaggagct gctcgatccg ttacgggcga tgctggcaaa aacacttgct
    34801 gcactgacgc ccggtaaact gaaatacagc ttcttttgta atagcggcac cgagtccgtt
    34861 gaagcagcgc tgaagctggc gaaggcttat cagtcaccgc gcggcaagtt tacttttatt
    34921 gccaccagcg gcgcgttcca cggtaaatcc cttggcgcgc tgtcggccac ggcgaaatcg
    34981 accttccgca aaccatttat gccgctgctg ccagggttcc gtcatgtgcc gtttggcaat
    35041 atcgaagcca tgcgcacggc ccttagcgag tgcaaaaaaa ccggtgatga tgttgctgca
    35101 gtgatccttg agccgattca gggcgaaggt ggcgtcattc tgccgccgcc gggatatctc
    35161 accgccgtgc gtaagctgtg cgatgagttc ggcgcactga tgatcctcga tgaagtgcaa
    35221 accggtatgg ggcgcacggg caagatgttc gcctgcgagc atgagaacgt acagccggac
    35281 atcctgtgtc tggcaaaagc cctcggcggc ggcgtgatgc cgattggcgc aaccatcgcc
    35341 actgaagagg tgttttccgt tctgttcgac aacccgttcc tgcataccac tacctttggc
    35401 ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca atgtgttgct ggagcagaac
    35461 ttaccggctc aggctgaaca aaaaggcgat atgttgctgg aaggtttccg tcaattggcg
    35521 cgggaatatc ccgatctggt acaggaagcg cgtggtaaag ggatgttgat ggcgattgag
    35581 tttgttgata acgaaatcgg ctataacttt gccagcgaga tgttccgcca gcgcgtcctg
    35641 gtggccggaa cactcaacaa cgctaaaacg atccgcattg aaccaccact gacactgacc
    35701 attgaacagt gtgaactggt gatcaaagcg gcgcgtaagg cgctggcggc catgcgagta
    35761 agtgtcgaag aagcgtaata tcaagtcgaa tggcgatacg acgtcgccat tcgatctttt
    35821 tatacaatac gtacgcccgc aggcatcatc cgctccggcg ttaacaacac gctttcactg
    35881 ccgtcatcgg tttccgcaca caacagcatg cattccgacg tttcaccgcg cattttcgct
    35941 ttttgcagat tacataagac caccaccgtt ttttccatca gctcttcttc gctgtagtac
    36001 ggcaccaggc tggtcacggt ttgcagtgtt ttttccccca catcaacctg tacgatgtac
    36061 agcttgtcgg cgttttcatg gcgtttcact tccacaatct ttccgacgcg catttccaga
    36121 cgtgcaaaat cagcgtaagc cacggtttcc attgtttcct cccttcgggt aaaattttac
    36181 taaactatag caaaagtttt tctcaatcct gtaggctaaa aatggagaat gcaggcgtga
    36241 tcacattcgt gagccgctgt gttaccgtta cagcgtcaaa gaaacgcgct ttatttactg
    36301 aaaacaggtg acccgataag cacttcctct acaatggggg cgcacatcag ggaaagtaaa
    36361 aaaggtaaac atggcaacac taaaagacat cgcaatcgaa gctggcgtat ccctggcgac
    36421 agtatccagg gtcttaaatg acgatccgac attgaatgtg aaagaagaga cgaaacatcg
    36481 cattctcgag atcgccgaaa agctggagta caagaccagt agtgcccgta aactccagac
    36541 aggtgcagtc aaccaacacc atattctggc tatctacagc taccaacagg agctggagat
    36601 caacgatcct tactatctgg cgatccgcca cggcattgaa actcagtgcg aaaagctggc
    36661 gatcgaactg accaactgtt atgaacacag cggattacca gacattaaaa acgtcaccgg
    36721 tattttaatt gtcggcaaac ccacgcccgc cctgcgcgcc gccgcctgtg cgttgaccga
    36781 caatatctgt tttatcgact ttcacgaacc cggcagcggt tacgatgcgg tggatatcga
    36841 tctggcacgc atcagtaaag aaatcatcga cttctatatc aaccagggcg ttaatcgcat
    36901 tggttttatt ggcggtgaag atgagcctgg caaggcggat attcgtgagg tcgcctttgc
    36961 ggaatatggc cgactgaaac aagtggtacg cgaagaggat atctggcgcg gaggcttttc
    37021 cagttcgtcg ggctatgaac tggcaaaaca aatgttggcg cgggaagact acccgaaggc
    37081 gttgtttgtc gcttctgatt ccattgctat cggcgtactg cgggcaattc atgaacgagg
    37141 cctgaacatc ccacaggata tttcgcttat cagcgtgaac gacatcccca ccgcgcgatt
    37201 tacctttccg ccgctctcaa ccgtgcgcat ccattccgag atgatgggaa gtcagggcgt
    37261 taacctgttg tatgaaaagg ctcgcgatgg tcgcgcgctg ccgctgttag tcttcgttcc
    37321 cagcaaatta aaactgcgcg gcacgacccg ctaaatcccc ttacacactg tccggcaatc
    37381 gtttttgccg gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa atgcatttac
    37441 ccgctacttt ttagtaaaaa ttttactaaa ctccccagca attacacaaa ctaccatcac
    37501 catgaatggt tccgatttct ctctaccggg aggccctatg aatcgctggg aaaacattca
    37561 gctcacccac gaaaaccgac ttgcgccgcg tgcgtacttt ttttcatatg attctgttgc
    37621 gcaagcgcgt acctttgccc gcgaaaccag cagcctgttt ctgcccttaa gcggtcagtg
    37681 gaatttccac ttttttgacc acccgctgca agtgccagaa gccttcacct ctgagttaat
    37741 ggctgactgg gggcatatta ccgtccccgc catgtggcaa atggaaggtc acggcaaact
    37801 gcaatatacc gacgaaggtt ttccgttccc tatcgatgtg ccgtttgtcc ccagcgataa
    37861 cccaaccggt gcctatcaac gtattttcac cctcagcgac ggctggcagg gtaaacagac
    37921 gctgattaaa tttgacggcg tcgaaaccta ttttgaagtc tacgttaacg gtcagtatgt
    37981 gggtttcagc aagggcagtc gcctgaccgc agagtttgac atcagcgcga tggttaaaac
    38041 cggcgacaac ctgttgtgtg tgcgcgtgat gcaatgggcg gactctacct acgtggaaga
    38101 ccaggatatg tggtggtcag cggggatctt ccgcgatgtt tatctgatcg gaaaacaact
    38161 aacacatatt aacgatttca ccgttcgtac cgactttgac gaagcctatt gcgatgccac
    38221 gctttcctgc gaagtggtgc tggaaaatct cgccgcctcc cctgtcgcaa cgacgctgga
    38281 atatagcctg ttcgacggcg agcgtgtggt gcacagcagc tccattgatc atctggcaat
    38341 tgaaaaactg accagcgcca gctttgcgtt tactgtcgaa cagccgcagc aatggtcggc
    38401 agagtctcct tatctttacc atctggtcat gacgctgaaa gacgccgacg gcaacattct
    38461 ggaagtggtg ccgcaacgcg ttggctttcg tgatatcaaa gtgcgcgacg gtctgttctg
    38521 gatcaataac cgttatgtga tgctgcacgg cgtcaaccgc cacgacaacg atcatcgcaa
    38581 aggccgcgcc gttgggatgg atcgcgtcga gaaagatctc cagttgatga agcagcacaa
    38641 catcaactcc gtacgtaccg ctcactaccc gaacgatccg cgtttttacg aactgtgtga
    38701 tatctacggc ctgtttgtga tggcggaaac cgacgtcgaa tcgcacggct ttgccaatgt
    38761 cggcgatatc agccgtatta ccgacgatcc gcagtgggaa aacgtctacg tcgagcgcat
    38821 cgttcgccat attcacgcgc agaaaaacca tccgtcgatc atcatctggt cgctgggcaa
    38881 tgaatccggc tatggctgta acatccgcgc gatgtaccac gcggcgaagg cgctggatga
    38941 cacgcgactg gtgcactacg aagaagatcg cgatgctgaa gtggtcgata ttatttccac
    39001 catgtacacc cgcgtgccgc tgatgaatga gtttggtgaa tacccgcatc cgaagccgcg
    39061 catcatctgt gaatatgctc atgcgatggg aaacggaccg ggcgggctga cggagtacca
    39121 gaacgtcttc tataagcacg attgcattca gggacattat gtctgggaat ggtgcgacca
    39181 cgggatccag gcgcaggatg acaacggcaa tgtctggtat aaattcggcg gcgactacgg
    39241 cgactatccc aacaactata acttctgtct tgatggtttg atctattccg atcagacgcc
    39301 tgggcctggc ctgaaagagt acaaacaggt tatcgcgccg gtaaaaatcc acgcgttgga
    39361 tctgactcgc ggcgagttga aagtcgaaaa taaactgtgg tttaccacgc ttgatgacta
    39421 caccctgcac gcagaggtgc gcgccgaagg tgaaacgctc gcgacgcagc agattaaact
    39481 gcgcgacgtt gcgccgaaca gcgaagcccc cttgcagatc acgctgccac agctggacgc
    39541 ccgcgaagcg ttcctcaaca ttacggtgac caaagattcc cgcacccgct acagcgaagc
    39601 cggacaccct atagccactt atcagttccc gctgaaggaa aataccgcgc agcctgtacc
    39661 tttcgcgcca aataatgcgc gtccgctgac gctggaagac gatcgtttga gctgcaccgt
    39721 tcgcggctac aacttcgcga tcaccttctc aaaaatgagt ggcaaaccga catcctggca
    39781 ggtgaatggc gagtcgctgc tgacccgcga gccaaaaatc aacttcttca agccaatgat
    39841 cgacaaccac aagcaggagt acgaagggct gtggcagccg aatcatttgc agatcatgca
    39901 ggaacatctg cgcgactttg ccgtagaaca gagcgatggt gaagtgttga tcatcagccg
    39961 cacggttatt gccccaccgg tgtttgactt cgggatgcgc tgcacctaca tctggcgcat
    40021 cgctgccgat ggccaggtta atgtggcgct ttccggcgag cgttacggcg actatccgca
    40081 catcattccg tgcatcggtt tcaccatggg gattaacggc gaatacgatc aggtggcgta
    40141 ttacggtcgt ggaccgggcg aaaactacgc cgacagccag caggctaaca tcatcgatat
    40201 ctggcgcagc accgtcgatg ccatgttcga gaactatccc ttcccacaga acaacggcaa
    40261 ccgtcagcat gtccgctgga cggcactcac taaccgccac ggcaacggtc tgctggtggt
    40321 tccgcagcgc ccaattaact tcagcgcctg gcactatacc caggaaaaca tccacgctgc
    40381 ccagcactgt aacgagctgc agcgcagtga tgatatcacc ctgaatctcg accaccagct
    40441 gcttggcctc ggctctaact cctggggcag cgaggtgctg gactcctggc gcgtctggtt
    40501 ccgtgacttc agctacggct ttacgttgct gccggtttct ggcggagaag ctaccgcgca
    40561 aagcctggcg tcgtatgagt tcggcgcagg gttcttttcc acgaatttgc acagcgagaa
    40621 taagcaatga ggatcatcga taacttagaa cagttccgcc agatttacgc ctctggcaag
    40681 aagtggcaac gctgcgttga agcgattgaa aatatcgaca acattcagcc tggcgtcgcc
    40741 cactccatcg gtgactcatt gacttaccgc gtggagacag actccgcgac cgatgcgcta
    40801 tttactgggc atcgacgcta ttttgaagtg cattactacc tgcaagggca gcaaaaaatt
    40861 gaatatgcgc cgaaagagac attacaggtc gtggaatatt atcgtgatga aactgaccgt
    40921 gaatatttaa aaggctgcgg agaaaccgtt gaggtccacg aagggcaaat cgttatttgc
    40981 gatatccatg aagcatatcg gtttatctgc aataacgcgg tcaaaaaagt ggttctcaaa
    41041 gtcaccatcg aagatggtta tttccataac aaataacaac tacggcggca aaaggagttt
    41101 gccgccgccg ctaccctact cattttcgga gatgtgttat gtctgatacc aaacgtaata
    41161 caatcggcaa attcggcttg ctctcgctga cttttgccgc cgtttacagc tttaacaacg
    41221 ttatcaataa taatattgag cttggactgg cctcggcacc gatgtttttc ctcgcgacga
    41281 ttttttattt tattcccttc tgtctgatca tcgcagaatt tgtttcgtta aataaaaact
    41341 cagaagctgg tgtctacgcg tgggtaaaaa gttcgctggg cgggcgttgg gcatttatta
    41401 ctgcctatac ctactggttc gtaaacctgt tctttttcac ctcgctgttg ccgcgcgtta
    41461 ttgcttatgc ttcgtatgcc ttcctcggct acgaatatat tatgacgccg gttgccacca
    41521 ccattatcag tatggtgctg ttcgccttct ccacctgggt ttccaccaac ggggcgaaaa
    41581 tgctggggcc aattacctcc gtcacttcaa cgctgatgct gctgttaacg ctctcctaca
    41641 ttttactggc aggtacggca ctggttggcg gcgtacagcc tgccgatccg atcaccgttg
    41701 acgcgatgat cccgaacttc aactgggcgt tcctcggcgt taccacctgg atctttatgg
    41761 ccgcaggtgg cgcggagtcc gtcgcggtgt acgttaacga cgtcaaaggc ggttcgaaat
    41821 cgttcgttaa agtgatcatc ctcgccggga tttttatcgg tgtgctgtat tccgtctcct
    41881 cggtgctgat taacgtcttc gtcagcagca aagagttgaa atttactggc ggctcggtgc
    41941 aggtattcca cggcatggcg gcgtattttg gtctgccgga agcgttgatg aatcgctttg
    42001 tcggtctggt gtcctttacc gcgatgttcg gttccctgct gatgtggacc gcaacgccgg
    42061 tgaaaatttt cttctccgaa atcccggaag gcatctttgg taagaaaacc gtcgaactta
    42121 acgaaaacgg cgttccggcg cgtgcagcgt ggatccagtt cctgatcgtt atcccgctga
    42181 tgattatccc gatgctcggt tccaatactg tgcaggatct gatgaatact attattaata
    42241 tgaccgccgc agcgtccatg cttccgccgt tattcatcat gctggcttac ctgaatttac
    42301 gcgccaaatt agatcacctg ccacgtgatt tccgtatggg ctcccgccgc accggtatta
    42361 tcgttgtttc aatgctgatt gcgatatttg ccgtagggtt tgtcgcttcg acattcccga
    42421 ctggcgcgaa tattctgacc atcatttttt ataacgtcgg cggtattgtt atcttcctcg
    42481 gctttgcgtg gtggaaatac agtaaatata taaagggatt aacggctgaa gagcgccata
    42541 ttgaagcgac gccagccagc aatgttgatt aagcagatta ataaaaaatg agcaggcatt
    42601 ttccctcttc ctggatgggg agagggaaaa tagtttctgc ctttatattt ttataattac
    42661 aacgataaaa ggctgtactt tttctttagc tcatggatta acacaatgaa attaatcact
    42721 gcaccatgca gagcattact tgctctgccg ttttgctacg cgttttctgc ggtcgctgaa
    42781 gaagctcgtc cggcagaaca tgacgacaca aaaacacccg ccattacctc gacatcttct
    42841 ccttcatttc gtttttacgg cgaattaggg gttggtggat atatggattt agagggtgag
    42901 aataaacata aatacagcga cggcacctat attgaaggtg gcctggagat gaagtacggc
    42961 tcctggttcg gcctgattta cggcgaaggc tggaccgtac aggccgacca cgacggtaat
    43021 gcctgggtgc ctgaccatag ctgggggggg ttcgaaggtg ggattaaccg tttctatggc
    43081 ggttatcgaa ccaatgatgg caccgaaatc atgctcagtc tgcgtcagga ttcctcgctg
    43141 gatgacctgc aatggtgggg cgatttcacg cccgatctgg gctacgtcat tcccaatacc
    43201 cgcgacatta tgaccgcgct gaaggtgcag aacttaagcg gcaactttcg ttatagcgtt
    43261 accgccacac ctgccggaca tcatgacgaa agcaaagcct ggctacattt tggcaagtac
    43321 gatcgctacg acgacaaata cacctatccg gcaatgatga acggttacat ccagtatgac
    43381 cttgccgaag gcatcacctg gatgaacggt ctggaaatca ccgacggcac agggcaactc
    43441 tatctgacgg gactgcttac ccctaacttt gccgctcgcg cctggcacca taccggacgc
    43501 gccgacgggc tggatgtacc gggaagtgaa agcgggatga tggtgagcgc catgtatgaa
    43561 gcgttaaagg gcgtttatct ctccaccgct tacacctacg ccaaacatcg ccctgaccac
    43621 gctgacgatg aaaccacctc tttcatgcag tttggtatct ggtacgaata cggcggcgga
    43681 cgtttcgcaa cggcttttga tagccgcttc tacatgaaaa atgcctctaa cgatcccagc
    43741 gaccaaatct tcctgatgca atatttttac tggtaataag gacagtaatc tcatgaaaat
    43801 aaaaactatt ttaacgccag tagcctgtgc tctgctgatg agcttttccg cccatgccgc
    43861 taatgctgac aattataaaa acgtgattaa ccgtactggc gcaccgcagt acatgaagga
    43921 ttacgattac gacgatcacc agcgttttaa tccgtttttc gatctcggag cctggcatgg
    43981 tcatctgttg ccagacggcc cgaataccat gggcggtttt ccgggcgttg cgctgctgac
    44041 ggaagagtac atcaacttta tggccagcaa tttcgaccgc ctgaccgtct ggcaggacgg
    44101 taagaaagtc gacttcacgc tggaggcata cagtattccc ggcgcgctgg tgcaaaaact
    44161 gacagcaaaa gatgtacagg ttgaaatgac tcttcgcttc gccacgccac gcacgtcact
    44221 actggaaact aaaatcatca gcaataaacc gctggatctg gtgtgggacg gcgaactgct
    44281 ggaaaaactg gaagcgaaag aagggaaacc gctttccgat aaaaccattg ctggcgaata
    44341 ccctgactac cagcgcaaaa tcagcgccac ccgtgatggc ctgaaagtca cctttggcaa
    44401 agtgcgcgcc acctgggatc tgctgacctc cggcgaatcg gaatatcagg tgcataaatc
    44461 cctgccggtg cagactgaaa tcaacggcaa tcgctttacc agtaaggcgc atatcaacgg
    44521 ttcgaccacg ctctatacca cctactccca cctgctgacc gctcaggaag ttagcaaaga
    44581 gcaaatgcag atccgcgata ttctggcgcg tccggcgttt tatctcaccg cctcgcagca
    44641 acgctgggaa gagtatctga agaaagggtt aaccaatccg gatgcgacgc cggaacagac
    44701 gcgcgtcgcg gtgaaagcga tcgaaacgct caacggtaac tggcgctcgc cgggcggggc
    44761 ggtgaaatat aacaccgtca cgccgtcggt gaccggacgc tggttctccg gcaaccagac
    44821 ctggccgtgg gatacctgga agcaggcgtt tgcgatggcg catttcaatc cggacatcgc
    44881 caaagagaat atccgcgcgg tcttctcctg gcagatccag cctggcgaca gcgtgcgtcc
    44941 gcaggatgtg ggctttgtcc ccgacctgat agcgtggaac cttagccccg agcgtggcgg
    45001 cgatggcggc aactggaacg aacgtaatac caaacccagc cttgccgcct ggtcagtaat
    45061 ggaagtgtat aacgtcaccc aggataaagc ctggttggca gagatgtacc cgaagctggt
    45121 ggcctatcac gactggtggt tacgtaatcg cgatcacaac ggcaacggcg tgccggaata
    45181 tggtgcgacc cgcgacaaag cccacaacac cgagagcggc gagatgctgt ttacggtgaa
    45241 aaaaggcgac aaagaagaga cgcagtctgg gctgaacaac tacgcccgtg tggtggagaa
    45301 aggccagtac gacagtctgg aaattccggc acaggttgct gcgtcgtggg aatcaggtcg
    45361 tgatgacgcc gccgtctttg ggtttatcga caaagaacag ctggataaat atgtcgctaa
    45421 tggcggcaaa cgtagcgact ggacggtgaa attcgccgaa aaccgcagtc aggacggaac
    45481 gttgctgggg tactcgctat tgcaggagtc ggtggatcag gccagctata tgtacagcga
    45541 taaccattat ctggcggaga tggcaaccat actcggtaag ccggaagagg ccaaacgcta
    45601 tcgccagttg gcacagcagc tcgcggacta catcaacacc tgtatgttcg acccgactac
    45661 ccagttctac tatgacgtgc gcattgaaga taaaccgctg ccgaacggct gcgcgggcaa
    45721 accgattgtc gagcgcggta aagggccaga aggctggtca ccgctgttta acggtgcggc
    45781 aacgcaggcc aacgccgacg cggtggtgaa ggtgatgctc gatcctaaag agttcaacac
    45841 ctttgttccg ctgggaacgg cggcgttaac caatccggct tttggcgctg atatctactg
    45901 gcgcgggcgc gtatgggtgg atcagttctg gtttggtctg aaagggatgg agcgttacgg
    45961 ttatcgtgat gatgccctga aactggcgga tacgttcttc cggcacgcca aagggttaac
    46021 cgccgacggc ccgattcagg aaaattacaa cccgctcact ggcgcgcagc aaggcgcacc
    46081 aaatttctcc tggagcgccg cgcatttgta tatgttgtat aacgattttt tccgtaagca
    46141 gtaatttttc ccgatgccgg acgactgtat taccgccgtc cggcatgacg aacaggcgta
    46201 ttctttttga atcccatcac aaaccccaca ctcccctttt cccttttctc cggcgacggc
    46261 taaattagaa ctcatccgac cacataacaa ttattttaca tactggacaa ttttatgagc
    46321 tacccgtcgc tgttcgcccc gctggattta ggcttcacca cgttaaaaaa ccgagtactg
    46381 atgggttcaa tgcacacggg actggaggaa tacccggacg gcgctgaacg gctggcagcg
    46441 ttttatgctg aacgcgcccg tcacggcgtg gcgttgattg tcagcggcgg tattgcacca
    46501 gatttaacag gcgttggcat ggaaggcggc gcaatgctca atgacgcaag ccagatcccg
    46561 caccatcgca ccataaccga agccgtacat caggaaggcg gcaaaatcgc cctgcaaatt
    46621 ttgcataccg ggcgctacag ctaccaaccg catctggtcg ctccttccgc attgcaggct
    46681 cccatcaacc gtttcgttcc ccatgagtta agccatgaag agatcctgca actgatcgac
    46741 aatttcgccc gctgcgcgca actggcgcgg gaggccggat acgacggcgt agaagtgatg
    46801 ggttccgaag ggtatttgat caacgagttt ctgaccctgc gcaccaacca gcgtagcgac
    46861 cagtggggcg gcgattaccg caaccggatg cgctttgcag ttgaagtggt gcgagcagtg
    46921 cgcgaacgcg tcggcaatga cttcattatt atctaccgtc tgtcgatgct cgacctggta
    46981 gaaggcggcg gtacttttgc agaaacggta gggctggcgc aggccattga agcggcgggc
    47041 gcgaccatta tcaacaccgg cattggctgg catgaagcac gtattccgac cattgccacg
    47101 cccgtgccgc gcggtgcctt tagctgggtc acgcgcaaac tgaaaagcca cgtctcgctg
    47161 ccgctggtga ccaccaaccg gattaacgat ccgcaggttg ccgacgacat tctctcgcgc
    47221 ggtgatgccg atatggtatc gatggcgcga ccgtttcttg ctgatgcgga actgctgtca
    47281 aaagcgcaat cggggcgagc cgatgagatc aacacctgta ttggctgcaa tcaagcctgc
    47341 ctcgatcaga tcttcgttgg caaagtcacc tcgtgcctgg tgaatcctcg cgcctgccac
    47401 gaaaccaaaa tgccgatcct tcccgccgtg cagaaaaaaa atctggcggt ggtcggcgct
    47461 ggtccggctg ggctggcgtt tgccattaac gcggcggcac gtggtcatca ggtaacgttg
    47521 tttgatgctc acagtgagat cggcggtcag tttaatatcg ccaaacagat ccctggcaaa
    47581 gaggagtttt acgaaacgct gcgctattac cgccggatga tcgaagtaac aggcgtgacg
    47641 ctgaaactca atcacaccgt gacggcggat cagttacagt cgttcgatga aacgatcctc
    47701 gctagcggga tcgtaccgcg cattccacct atcgacggaa tcgatcatcc gaaggtgttg
    47761 agttatctcg acgtactgcg cgacaaagcg cctgtgggca aaaaggtagc aatcatcggt
    47821 tgtggcggga ttggttttga tacggcgatg tttttaagtc agccgggtga gtccaccagc
    47881 cagaacattg ccgagttctg taatgagtgg gggatcgaca gcagcctgca acaggctggt
    47941 ggcttaagcc cgcagggaat gcagatcccg cgtagcccac ggcagattgt gatgctccag
    48001 cgcaaagcca gcaaaccggg acaggggtta ggcaaaacca ccggctggat ccatcgcacc
    48061 accctgctct cgcggggtgt gaaaatgatc ccaggcgtaa gttatcagaa gattgacgat
    48121 gacgggctgc atgtggtgat caacggcgaa ccgcaggtat tagcagtgga caatgtggtg
    48181 atctgcgcag ggcaagagcc aaaccgcgcg ctggcgcaac cgctgattga tggcgggaaa
    48241 actgtgcatt taattggcgg ctgcgatgta gctatggagc tggacgcacg acgggcaatt
    48301 gcccagggaa cacggctggc gctggagatt taaatcgtta tttgaagcgc cggatgcgac
    48361 gcatccggca cgtcgcgtta atcaataaac aaggcgggag aaatgccgaa tcgcgttgcc
    48421 agttttttag cgtgttccag cgttaatttc ctcttcccgc tcaaaacgcg tgacaccatg
    48481 gatttactgc caatttccgg aagatcggaa agggttaaac catattgatc cataagggta
    48541 cgaatcacgg ctataccgcc aggcatggct tgagccatgg cattaaattc agcaaattcg
    48601 ggcgctgatt cttcccacgc ggttattttg gcacacacca gatccagcaa gggattttca
    48661 ggatcgttga gcagcagatg atcgaccagt tccagcgcct gggcgtattg ttcctcgttc
    48721 tgaatacccg ccagaaaagg tgccacagca gttagctttt ctcctgcttg caagatgtcg
    48781 gcaatcgcaa tcattttttc cccttagtac gatgaacggc ggtaaagaaa tcgtattctt
    48841 tatgcgtcat aacttcacgt atgtagcact tttgcgattc aaaaaagacc attgctacaa
    48901 cacgtaattc attgcccccg acattgaaaa cataatgctt atccagatat ttgaagttat
    48961 ccagagatgg gaatactgct tttaatgatt caggtttttt gaaatatccc ttagcaatcg
    49021 tgttccccag agccaccaac tccgttttat gttgcgggta tttttccgca gcatctttca
    49081 atgctttttg agttatcagg tgcattcttc atcacgtccg ttgacaaatt ggcaatatga
    49141 taacatccgt tgacagattg gcaacgaatg aatttatctg tgtgacggaa gaataaacgt
    49201 agaaaaatgc taaacaagag aatgtagagg atggtgacag ggattttttg ttttatggaa
    49261 acgcgattcc agaactgggc agacattgcc ggatgcgacg cttgccgcgt cttatccgac
    49321 ctgggggtaa tgtgctgaac gtatgccaga taaggcgttt acgccttatc tggcaggtag
    49381 ctgccaatct taccgacgac gccccagctt cactgctttc agcaccacaa atttattatt
    49441 cgtggcgatg gtggtgcagt tgccgaaaat cttcttcagt ttatggaagt aatccaggtg
    49501 acggttggca acgatataca gctcgccgtt gattttcaga cagcggcggg cgtggtggaa
    49561 catctcccag gcgacgttat cggtcagcgc atgttgctga tgaaacggcg ggttgcagag
    49621 cacagcatta aagcggaaag gctccacgcc ggagagcgcg ttgttgatca taaactcgca
    49681 gcgatccaac gcctctggca tgttggtttc aacgttcaaa cggctggaag caaccgccat
    49741 cggcgattca tcgacaaaca ccactttcgc ctgcgggttt ttatcaagca gcgtcagacc
    49801 aataacgcca ttaccacaac cgagatcgac aatctcacct tcgagattct ctggcagatg
    49861 ctgcataaag aagcgcgcgc caatatccag cccggtgcgg gagaagacat tcgcatggtt
    49921 gtggatagtc cagtcagtac cttccagttt ccagctaacg gtctgcggcg catcggccag
    49981 cggcggctcg ttgaaagtac agttaattaa gcgtgccttc ttccatgcca gcgtggtggt
    50041 ggtcgggccg agcacttttt cgaacagttc cagggtggaa gtgtgaatgt cacgggcttt
    50101 ggtaccggca ataatgcgcg tatctggcgt gaccactttg cgcagcgcgc gcagttgctg
    50161 ttccaacaat gccagtgttt tcggtacttt gatcagcacc actcccggct gttgcgggta
    50221 gtcagcggtg ctgtcgagaa acttcacgct cgactcatca atcccgttga ggcgtaaatt
    50281 ctcgcgcgtc gccagttcgc tgatgtatga gtcgccaatg ctgtatggct tatgttccgc
    50341 cagcgcacaa cttaacgcac caaaggcatc attcaggatc aacaccgggc cgcggatttc
    50401 tgtgtcgtcc aactgttgca gcaaatattc atccgccgct tcccacgcct gtagtgggtt
    50461 aacgtcatcc gtcgccggaa aacgttgtag tgtcagtgaa cggaaaccgt tgtctaagtg
    50521 gctcatcggc cctcctgaat gataaaattt cgacgttgcc cctgaaaagg gtgcgtgagt
    50581 ataccctttt tctctttttc gtgggcggtt atgagcaatc ttacttatct tcagggttat
    50641 cccgagcacc tactttccca ggtgcgaacg ctgattaacg aacagcgtct gggcgatgtg
    50701 ctggcaaaac gctatccggg aacacacgac tactccaccg ataaagccct ctggcaatat
    50761 actcaggatc ttaaaaatca gtttctgcgc aatgccccgc cgatcactaa agtgatgtat
    50821 gacaacaaga tccatgtgct gaaaaacgcg ctcgggttac ataccgctgt ttcgcgtgtg
    50881 cagggcggca agctgaaagc aaaggcggag atccgcgtcg ctaccgtgtt tcgcaacgcg
    50941 ccggaaccgt ttttgcgcat gatcgtggtg cacgagttgg cgcacctgaa ggagaaagag
    51001 cataacaaag cgttctatca attgtgctgc catatggagc cgcagtacca ccagcttgag
    51061 ttcgacaccc ggctgtggct aacgcagttg tcgcttgggc aggacaaaat ctgaaaatga
    51121 tgtcaggcgt tatcttttct tcctatagcg cctaaaatcg acctcccccc tttcgttaag
    51181 gataacgacc atgcagcccc gccttcttct ccgtatttgt ttttctcgcc ggactcttaa
    51241 gattggctgt ttattgttgc ttatagcagg agcgaccatt tttattgccg atcgcgttat
    51301 ggtcaacacc agcaagcagc tgacctggag cgatgtcaac gcagttccgg cgcgtaacgt
    51361 gggtttattg ctgggggcga ggccgggtaa tcgctacttt acccgacgta ttgataccgc
    51421 agcagcgttg taccacgccg gaaaagtgaa atggctactg gtaagtggcg ataacggacg
    51481 taaaaattac gatgaagcct caggtatgca gcaggctttg atcgctaaag gcgtaccggc
    51541 gaaagtgatc ttctgcgact atgccggatt ctcaacgctt gattccgtgg tgcgtgccaa
    51601 aaaggtgttt ggtgaaaacc atatcaccat tatttcgcaa gaatttcata accaacgcgc
    51661 catctggctg gcaaaacagt acggcatcga cgctatcggc tttaacgcgc cagacctcaa
    51721 tatgaagcac ggtttttata ctcagttacg ggagaaactc gccagagtca gcgcggtgat
    51781 tgacgccaaa atcctccatc gccagccgaa atatctcggg ccatcggtca tgattgggcc
    51841 gtttagtgag catggctgtc cggcgcagaa ataatgcgta tctgcgcacg tcgaagatga
    51901 aaaaggcgtg ctacattgac ggcagaatcc ctttatggag tatccacgcg ttatgatacg
    51961 tttcgctgtg attggtacga actggatcac tcgccagttc gtcgaggccg cccatgagag
    52021 cggtaaatac aagttaaccg ccgtatattc ccgcagcctt gaacaggccc agcactttgc
    52081 caatgatttt tctgtcgagc atctgtttac gtcgctggaa gcgatggcag aaagcgatgc
    52141 cattgacgcg gtgtatattg ccagcccgaa tgccctgcac ttttcccaga cacaactttt
    52201 ccttagccac aaaattcatg tgatttgcga gaagccgctg gcatcgaatc tggcggaagt
    52261 ggatgccgcc attgcctgtg cgcgggaaaa tcaggtggtg ctgtttgagg catttaaaac
    52321 cgccagcctg ccgaactttc atctgttacg ccaggcactg ccgaaagtcg gcaaactgcg
    52381 taaagtcttt ttcaactatt gccagtattc ctcgcggtat caacgttacc tggatggtga
    52441 gaatcccaac acctttaatc cggcattctc taacggttca attatggata tcggctttta
    52501 ctgcctggcg tcggcggtgg cattattcgg tgaaccgaaa agcgtgcagg caaccgccag
    52561 tttgctggca agcggtgtgg atgcccacgg cgtggtggtg atggattacg gtgatttcag
    52621 cgttacgttg cagcactcaa aagtgagcga ttctgtcctg gcaagcgaga ttcagggcga
    52681 agcaggatcg ctggtgattg aaaaactgtc tgaatgccag aaagtttgct ttgttccgcg
    52741 tggcagccag atgcaggatc tcacccaacc gcagcatatt aataccatgc tctacgaagc
    52801 agagctgttt gctgaactgg tggatgaaca tctggtggat catccagggc tggcggtcag
    52861 tcgcatcacc gccaaactgc tgaccgagat ccgccgccag actggggtga tttttccggc
    52921 agatagcgta aaactataat tgccaaagta aagcagtgta aaaggcatgt aacagaccat
    52981 tgactgactg aatggtctgt catactttgt tacctgcaaa ggggagtaac ttcattgccg
    53041 gtcgatcgtc attacgatgt gtgaaaaacc acatccggtc accgggcaac ccgaaaggaa
    53101 tacgcagacg tattcctttt ttgttgtaag tgagaccttg ccggaaggcg aggtctatgc
    53161 ataaaaagca gcggctgacg tcttccgacg ttggccgttt ttttatgtgt aaggaacttc
    53221 tatgaatact gtcggcacgc cgttgctatg gggcggattc gctgttgttg tcgccattat
    53281 gctggctatc gacctgttgt tgcaggggcg tcgtggggca catggcatga ccatgaaaca
    53341 ggctgcggcc tggtcgctgg tctgggtgac gctgtcgtta ctgtttaacg ccgctttctg
    53401 gtggtatctg gtgcaaaccg aaggtcgcgc cgttgccgat ccacaggcac tggcctttct
    53461 cacgggttat ctgattgaga aatcgctggc ggtcgataac gtctttgtct ggctgatgtt
    53521 gttcagctat ttctctgttc cggcggcgtt acaacggcgc gtgctggtgt atggcgtgct
    53581 cggggcgatt gttctgcgta ccatcatgat cttcactggc agctggctga tttcgcagtt
    53641 cgactggatc ctgtatatct tcggtgcctt cctgctgttt accggcgtga agatggcgct
    53701 ggcccatgaa gatgaatcag gcattggcga caagccgctg gtgcgctggc tacgcggtca
    53761 tttgcgcatg accgacacca tcgacaacga gcatttcttt gtgcgtaaga atggcttgct
    53821 gtatgccacg ccgctgatgc tggtgctgat tctggtggaa ttgagcgacg tgattttcgc
    53881 cgtggatagc attccggcta tcttcgccgt gaccactgac ccgttcattg tgctgacctc
    53941 aaacctgttt gcgatcctcg gcctgcgtgc gatgtatttc ctgctggcgg gcgtagcaga
    54001 acgtttctcg atgctcaaat atggcctggc ggtgattctg gtgtttatcg gtatcaagat
    54061 gctgattgtc gacttctacc atattccaat cgccgtctcg ctgggcgtgg tgtttggcat
    54121 tctggtgatg acgtttatta tcaacgcctg ggtgaattat cggcatgata agcagcgggg
    54181 agaatgattc gctgtatatt aagccattac tagagataac tcattccaga caaaggtgag
    54241 ctattaatta tgtaatcggc ttatagctct ttacaaaaag accgcacggg tgttggtggc
    54301 gtcaaaatcg ccgaagcgtt ccctggaggc cggggcgacg cgcatggatg cgcggcgagg
    54361 gcgcatctta cagggacgtt cccttgcgcc cgacccggta gccgcaaggg ataagctgag
    54421 ggaaccgcgc agcggcgatt ttgttcgcca gagcctgggg gtgcaggggg cggcggcgat
    54481 tggccgcccc ctgcgcgctc cctgcggcat tgactttata tgacatagca cattaaagga
    54541 gcgcaaccgt tctcatattc caaatagttt tgcccatatg ttatctaatc agccttagta
    54601 gccagtcctt ccctctgttt atgtcaaata aatgtaacca tgaagttgca aaatatgacc
    54661 tctctttaaa atccagcatt tttcgcttcc cgaagctgta actttcctta tactcgacct
    54721 tgcaaacact ttgttacatc ctgaaagatg cgtcgacaga acgcaccagg gatgtgcgac
    54781 aacacaatga aaggatcgaa aaatgactac gcaacgttca ccggggctat tccggcgtct
    54841 ggctcatggc agcctggtaa aacaaatcct ggtcggcctt gttctgggga ttcttctggc
    54901 atggatctca aaacccgcgg cggaagctgt tggtctgtta ggtactttgt tcgtcggcgc
    54961 actgaaagcc gttgccccca tcctggtgtt gatgctggta atggcgtcta ttgctaacca
    55021 ccagcacggg cagaaaacca atatccgtcc tattttgttc ctctatctac tgggcacctt
    55081 ctctgctgct ctggccgcag tagtcttcag ctttgccttc ccttctaccc tgcacttgtc
    55141 cagtagcgcg ggtgatattt cgccgccgtc aggcattgtc gaagtgatgc gcgggctggt
    55201 aatgagcatg gtttccaacc ccatcgacgc gctgctgaaa ggtaactaca tcgggattct
    55261 ggtgtgggca attggcctcg gtttcgcact gcgtcacggt aacgagacca ccaaaaacct
    55321 ggtcaacgat atgtcgaatg ccgtcacctt tatggtgaaa ctggtcattc gcttcgcacc
    55381 gatcggtatt tttggtctgg tttcttctac cctggcaacc accggtttct ccacactgtg
    55441 gggctacgcg caactgctgg tcgtgctggt tggctgtatg ctgctggtgg cgctggtggt
    55501 taaccccttg ctggtgtggt ggaaaattcg tcgtaacccg ttcccgctgg tgctgctgtg
    55561 cctgcgcgaa agcggcgtgt acgccttctt cacccgcagc tctgcggcga acattccggt
    55621 gaatatggcg ctgtgtgaaa agctgaatct ggatcgcgat acctattccg tttctattcc
    55681 gctgggtgcc accatcaata tggcgggcgc agcaatcacc attaccgtgt tgacgctggc
    55741 tgcggttaat acgctgggta ttccggtcga tctgcccaca gcgctgctgt tgagcgtagt
    55801 ggcttctctg tgtgcctgtg gcgcatccgg cgtggcgggg ggttctctgc tgctgatccc
    55861 actggcctgt aatatgttcg gtatttcgaa cgatatcgcc atgcaggtgg ttgccgtcgg
    55921 ctttatcatc ggcgtattgc aggactcttg cgaaaccgcg ctgaactctt caactgacgt
    55981 gctgttcact gcggcagctt gccaggcaga agacgatcgt ctggcaaata gcgccctgcg
    56041 taattaattg tttaacccct ttcgtctacg gcggaagggg ttttctctac tttaaacgga
    56101 tcaattcccc ttttctgcat ccgccagaaa cgaatgatat tcagaccatt catcagcaga
    56161 aaactcccct caatcatcgt gccgcctatc gaccccgccc agaagttgtg aatcacccag
    56221 caacacgttg aaaaccacat tacgcagcgc atggtcagcc ctttacagcg gaacagcgcc
    56281 caggtactga caatcgtgcc gattaccggc agtagttcga caggatgatg gaacttcgcg
    56341 aggccaattc cgccagtcag cacaataaaa atcgccatta cccataagct gcgcgtgcgt
    56401 aaagtaatca gcgtacgaat ggcattaagg atggcactgg caccagcggg ataggtgccc
    56461 agaagaaaaa aatgtacgcc aataacggcg ctatagaccg aaagctgctt tttgaagcga
    56521 cgttcgtcac gattgaaaaa tgttgtgata ccgatcagaa aggcgatgac acccacgccc
    56581 tgggccagcc aatacgcggt catgataaat ccttagcagg tatggaaaag caaacggcgc
    56641 ttcacattat gaaacgccgt tttttattaa caactcattt cgactttata gcgttacgcc
    56701 gcttttgaag atcgccagtt cgcggaagtc gttacgctcg ttgcaggttt gtttaccatt
    56761 ggcaaactca acgatggtat cgataaattc ttccagcaac tgcggcatcg ctttaccgtg
    56821 gatcagctga cccgcgtcaa agtcgatcca gtgttttttc ttcgccgcca gttcactgtt
    56881 ggtggcgatt ttcaccgtcg gcacaaatcc accatacggc gtgccacgac cagtactgaa
    56941 cagcaccata tggcagcccg cacccgccag ggcgctggtc gctacggcat cgttacccgg
    57001 cgcacttaac aagttcagcc ccggtgtttt cagacgctca ccgtaacgca gcacgtcaac
    57061 cacgacgctg gaacccgctt tctgggtaca gccaagtgat ttgtcttcca gcgtggtgat
    57121 accgcccgct ttgttccccg gcgatgggtt ttcatagatc ggctggtcat gggcaataaa
    57181 gtactgcttg aagtcattga ccatggtgac cagtttttca aacgttgctt cgtcgcggca
    57241 atggtccatc agcaactgct ctgcgccaaa catctccggc acttcggtca gtacggtagt
    57301 accgccgtta gcaatcacgt agtcagagaa acgccccagc atcgggttag cagtaatacc
    57361 agaaagaccg tcagaaccac cgcactccag accaaacttc agttcgctga gtttgcctgg
    57421 ctcgcgcttg tcgttgcgca tcacgttata cagctgatgc agatgctcga tcccggcttc
    57481 gatctcatca tcctgctgtt ggcagatcat gaaatgaacg cgttcaggat cgatatcgcc
    57541 cagcgtttca cggaatgcgg taacctggtt gttttcacag cccagaccaa tcaccagcac
    57601 tgcgcctgcg ttcgggtggc gcaccatgtt ttgcagcatg gtgcgggtat taatgtgatc
    57661 gtcgcccagc tgtgagcagc cgtaggtgtg gctgaagagg aacacgccgt cggtaccttc
    57721 agcgttgttg gtctctttca gaaaacggtt ctggatctgc cgcgcgatgc cgttaacaca
    57781 gcccacggtt ggcaggatcc acagctcatt gcgcaccccg acatcgccgt tagcgcgacg
    57841 atagatctgc acttcacgat ctgccgcttg cgcaggcaga tcctgaaaat caggttgata
    57901 gcgatactga tccagatcgc tcagattcgt gcgcgtattg tgggcgtgaa cgtgttcccc
    57961 cgccgcaata tccgccaatg cataaccaat cggcaggcca tatttgatga cgttggcccc
    58021 ttttgcgata tccgttaacg caaatttatg tccacgagca acatcctggc gcagcgtaac
    58081 agtctggttg tcgacactga cttctgtgcc ttccgccaaa tctgctaaag cgaccgcgac
    58141 gttatccagc gcatggatct tgatgtattg catatcaacc ccagacctta gttcagttca
    58201 atggcgaagt agtcacgcgc attgttaaag caaatatttt tcaccatctc gcccagcagg
    58261 ttgatatccg ccggcgcttc gcccgcttcc acccagcgac caatcatctg gcacagaatg
    58321 cggcggaagt attcgtgacg ggtgtatgac aggaagctac ggctgtcagt cagcataccg
    58381 acaaagcggc tcagcagacc gagctgcgcc agttgggtca tctgacgttc cataccgtct
    58441 ttctgatcgt taaaccacca gccggaaccg aactgcattt tgcccggcat accttcgccc
    58501 tggaagttac cgatcatggt gcccagcact tcgttatcgc gcgggttcag gcagtagaga
    58561 atggttttcg gcagcaggtt ttcttcgttc tgcttgctca gcaacttaga cagctcttct
    58621 gccatcggac ggtcgttgat ggaatcaaaa cctacatccg gccccagcag tttgaactga
    58681 cgcaggttgt tattacgcag tgcgccaatg tggtactgct gtacccagcc gcgacgtgcg
    58741 tattcggcac caaggaacac cagcaccgca gttttgaact gtgccacttc gtgctcgctc
    58801 agggtttcgc ctgccagacg gcgcgccaga atgcggtcca gttccgcttc gttcgcttca
    58861 gcaaacatca ccacatccag cgcgtggtca gacactttac agccgtgagc ggcgaagtga
    58921 tccagacgtt tggtcagggc agtttgcaga tcagcaaagc ggcggatgtc ggtatcagag
    58981 acttcaccca gcttcgccat gtagtcgtta aaggtcgcct gttcgatgtt aaaggctttg
    59041 tccggacgcc agctcggcag cactttgatg gtgaaagagc cgtctttggc gatctctgcg
    59101 tgatgctcca gagaatcgat cgggtcatca gtggtgccga ccattttcac gttcatctgc
    59161 tgcatgatgc cgcgcgcgga gaagttatcc tgcgccagca gttcgttaca ttcgttccag
    59221 atttcatcgg cagttgacgg agaaagcaat ttgccagtga taccgaacgg acgacgcagt
    59281 tcgaggtgcg tccagtggta taacgggttg ccgatagtgt gcggaacagt cgccgcccag
    59341 gcgtcaaatt tttcacggtc agacgcatca ccagtacaca gacgctcagc cacaccgttg
    59401 gtacgcatag cgcgccattt gtagtgatcg cccttcaacc agatgtcata cagattttta
    59461 aaacgatagt cttccgcaat ctgctgcggc ggcaaatggc aatggtaatc gaaaatcggc
    59521 tggtcttttg cgtagtcgtg atacagacgg cgggcaaatt cggtatctaa caggaaatct
    59581 tcagtcataa acggagtcat tttcgtcttc ctctcaacga gtgagctagc ttgcttatgg
    59641 tgcgatgctg acaaagttat cacaccaatt tccagagtcc gaagatattt tcgtgagata
    59701 gatcaataaa cgtagttaaa aaaattactc tcaaagtggt aaatctcgct gcaggccgcg
    59761 ccagtactgg cctcgctgtc gtcaggtaat gtccctacaa atattcccac atttgtgatg
    59821 gctctcacct tttaaagttg tatgacaagt tatctttctg ccgtcgcaaa tcataagtcg
    59881 acggaatgca aattgccgat tcattcattt gttagatgaa tcgggttaac cggtacggaa
    59941 gccgaattag cacgaaactt tcatggcaac gttcggggcg tgccggtttt ttttcggtta
    60001 cccggtcgta actaacatct tcagcctctg gcgggatgat ggccgcgctt cctgcggata
    60061 taacaaaacg atgaggtttt acatgcgtaa aattaaaggg ttacgttggt atatgatcgc
    60121 actggtgacg ctcggcaccg tgcttggtta cctgacgcgt aacactgtgg cggcagctgc
    60181 gccaactctg atggaagagt taaacatctc cacccaacag tattcctata tcatcgcagc
    60241 ctattctgct gcttatacgg tcatgcaacc ggtagcgggt tatgtactgg atgtgctggg
    60301 tacgaaaatc ggttacgcaa tgtttgctgt actgtgggcc gtattctgtg gtgcaaccgc
    60361 gctggcaggt agctggggtg gcctggcgat tgctcgtggt gcggtcggtg ccgcggaagc
    60421 agcgatgatc ccggcgggtc tgaaagccag ctccgaatgg ttcccggcga aagagcgttc
    60481 catcgcagta ggctacttta acgtaggttc ttctattggt gcgatgattg cgccgccgct
    60541 ggttgtatgg gcaatcgtga tgcacagctg gcagatggca tttatcatct ccggtgcatt
    60601 gagctttatc tgggcaatgg catggctgat tttctataaa catccgcgcg accagaagca
    60661 tctgaccgat gaagaacgcg actacattat taatggtcag gaagcccagc accaggtgag
    60721 cacggcgaag aaaatgtccg ttggtcagat cctgcgtaac cgtcagttct ggggtattgc
    60781 actgccgcgt ttcctggcag aaccggcctg gggtactttt aacgcgtgga tcccgctgtt
    60841 catgtttaaa gtttacggct ttaacctgaa agagatcgcg atgtttgcct ggatgccgat
    60901 gctgtttgct gacctcggtt gtatcctcgg tggttacctg ccgccgctgt tccagcgttg
    60961 gtttggtgtt aacctgatcg tttctcgtaa gatggtcgta acgctgggtg ctgtgctgat
    61021 gattggcccg ggtatgatcg gtctgttcac caacccgtat gtcgcaatta tgctgctgtg
    61081 tatcggtggt tttgctcacc aggcactgtc tggcgcgctg attacgctct cttctgacgt
    61141 gttcggtcgt aacgaagtgg caacggcaaa cggtttaacc gggatgtccg catggctggc
    61201 aagtacgctg tttgcgctgg ttgtcggtgc actggctgac accatcggct tcagcccgct
    61261 gttcgcagtt ctggcagtat ttgacctgct gggtgcgctg gttatctgga cggtgttgca
    61321 gaacaaaccg gcgattgagg tggcgcagga aacacataac gatcctgcac cgcaacatta
    61381 aggcttcgtg taagttttag tttcccttca aagccgcctt ctcaggcggc tttttcatca
    61441 ctgtgagtag agctaaactc gcaaaagtgg tataacaaat atagtctgcc gtatcatgcc
    61501 tggagcgcat atggaaatca ctgaaccacg ccgtttgtat caacaacttg ccgctgatct
    61561 gaaagagcgc atcgaacagg gcgtctatct ggtgggtgat aaactgcctg ctgagcgctt
    61621 tattgccgat gaaaagaacg tcagccgtac ggtagttcgt gaagccatca tcatgctgga
    61681 agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt cacgtggttt ccaaccagcc
    61741 gcgtcatcag caggcggcgg acaataatat ggaattcgcc aattacggtc cgtttgagtt
    61801 gctccaggct cgccagctca tcgaaagtaa tattgccgag ttcgcggcaa ctcaggtaac
    61861 gaaacaggac atcatgaaac tgatggccat ccaggagcag gcgcgcggcg aacaatgctt
    61921 ccgtgattcc gagtgggatt tgcagttcca cattcaggta gccctggcga cgcagaactc
    61981 cgccctggcg gctatcgttg aaaaaatgtg gacccaacgt agccataacc cgtactggaa
    62041 aaaactacac gaacacattg atgcccgtac tgtcgataac tggtgcgatg accacgatca
    62101 gatcctcaag gcgttaattc gcaaagatcc tcatgctgcc aagctggcaa tgtggcagca
    62161 tctggaaaac accaaaatca tgttatttaa cgaaaccagc gacgatttcg agttcaatgc
    62221 cgaccgctat ctgttcgcag aaaacccggt agtgcatctc gatacggcca ccagcggcag
    62281 caaatgaaat ttccatccga caggcgctgc aatgcgcctg tttgcttcgt cggtaagcga
    62341 aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc ctcccctgca atcagcaaaa
    62401 tcagactcca cggacatgta attttgataa cgaacaacgt tgacctttgt tacaattaga
    62461 ttcaatttga atttatgctt ttgaatgctt tcttatctca cgatttaaca gggaatagtt
    62521 caggctgtgt tgatgtatca aacccgcaaa acataccaaa acagcaataa cattgcggta
    62581 gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc aggaataatg aatggaactt
    62641 ttgacccaat tgctgcaagc cctgtgggcg caggattttg aaaccctggc caatccgtcg
    62701 atgattggca tgttgtattt tgtcttgttt gtaattttgt tccttgaaaa cggcttgctt
    62761 ccggcggcct ttttaccggg cgacagttta ctggtgttgg tcggcgtatt gattgcgaaa
    62821 ggcgcgatgg gctatccgca aacgattctg ctgctgaccg ttgccgccag cctcggttgc
    62881 tgggtcagct atattcaggg gcgatggttg ggcaataccc gcactgtaca aaactggcta
    62941 tctcatttac ccgcgcatta tcatcaacgc gcacaccatc tttttcataa acacggttta
    63001 tcggcgctgt taattggtcg ctttattgca tttgtcagaa cactgctgcc gacgattgcc
    63061 ggattatcag ggctgaataa cgcgcgcttt cagttcttca actggatgag cggtctgctg
    63121 tgggtattga tcctgacaac tctgggttac atgctcggca aaacgccggt atttttaaag
    63181 tacgaggacc agctgatgtc atgcctgatg ctgctcccgg tggtgctgct ggtgtttggc
    63241 ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg gaaatcgggg gtaagggatg
    63301 caaatacctc gcatgtcgct tcgccagcta gcctggtccg gcgctgtttt acttctggtc
    63361 ggcacactgt tgctggcctg gtccgcggtt cgccagcaag agtctacgct ggcgattcgt
    63421 gccgttcatc aagggacaac gatgccagac ggtttttcaa tctggcatca ccttgacgct
    63481 cacggcattc ctttcaaaag tatcaccccc aaaaatgaca ctctgttaat tacctttgac
    63541 tccagcgacc agagtgccgc cgcaaaagcg gtcctcgaca gaacattgcc ccatggctac
    63601 atcatcgcgc agcaggacaa taatagtcag gctatgcagt ggctgacccg gttgcgggat
    63661 aactctcatc gcttcggata acttcctgtt atccgaaaca tatcactgac tttggtgatt
    63721 tcaccgtaac tgtctatgat taatgaggcg gtggttaaca ctaccgcttc gttgtctgga
    63781 caatcgttcc tttgtaatag gtccgaatca caatggaagg ttcaagaatg aaataccgca
    63841 tcgctttagc tgtttctctc tttgctctta gtgccggtag ttatgccact accctgtgtc
    63901 aggaaaagga gcaaaatatc cttaaggaga tcagctatgc cgaaaaacac caaaaccaga
    63961 atcgtattga cggtctgaat aaagccctga gtgaagtccg ggccaactgt tcagatagcc
    64021 agctgcgtgc cgatcatcag aagaaaatcg caaagcagaa agatgaggtg gcggaacgcc
    64081 agcaagattt aaccgaggcg aagcaaaaag gcgatgccga taagattgcc aaacgcgaac
    64141 ggaaactggc agaagcgcag gaagagctga aaaagctgga agcgcgcgac tactaactca
    64201 caatagtcac tacttactca cctggagaaa actatgtcga aagaacacac tacggaacat
    64261 ctgcgtgctg agttgaaatc cctttccgat acgctggaag aggtgcttag ctcatctggc
    64321 gagaagtcga aagaagagtt gagtaagatt cgtagcaaag cggagcaggc actgaaacag
    64381 agccgttatc gcctgggtga aaccggtgat gccatcgcca aacaaacccg tgtcgcggcg
    64441 gcgcgtgccg atgagtatgt gcgtgaaaat ccatggacgg gcgtgggcat tggcgctgca
    64501 atcggtgtag tgctcggcgt tctgctatcg cgtcgttaat tatggcggac actcatcacg
    64561 cgcaagggcc cggtaaaagc gttctgggca tcgggcagcg aattgtttcc atcatggttg
    64621 aaatggtgga aacacgtctg cggctggcgg tggtggagct ggaagaggaa aaagcgaatc
    64681 tctttcaact tttactgatg ctgggcctga cgatgctttt cgctgcattt ggtcttatga
    64741 gcctgatggt gctgattatt tgggcggttg atccgcaata tcgcctgaat gcgatgattg
    64801 ccaccaccgt ggtgttgctg ctactggcac tgattggcgg tatctggacg ttacgtaaat
    64861 cgcgtaaatc caccttgctg cgccatacac gccatgagct ggcaaacgat cggcagctgc
    64921 tcgaggagga gtcccgtgag cagtaaagtc gaacgtgaac gacgtaaggc gcaactgctt
    64981 agccagatcc agcaacaacg gctggatctt tctgccagtc gtcgtgaatg gctggaggca
    65041 acaggcgctt acgatcgtcg ctggaatatg ctgctaagtc tgcgctcctg ggcgctggtt
    65101 ggcagtagcg tgatggcgat ctggacgatt cgccatccta atatgctggt ccgctgggcc
    65161 agacgcggtt ttggcttatg gagcgcctgg cgtctggtta aaacgaccct caagcagcaa
    65221 cagcttcgcg gttaataacc tctctggccg gagccattcc ggccttatcc ctcaaatttt
    65281 ttgaagattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
    65341 tccatcgaca gcaacgacgc taataccgcg cccttgcaca aaaaaacaat cagcagcctg
    65401 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgc
    65461 attttaatgc cgattctgtt tattaccgca ggctggggaa aaattactgg ctacgcgggt
    65521 acccaacaat atatggaagc gatgggtgtc ccaggcttta tgctgccgct ggtgattctg
    65581 cttgagtttg gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cacagccctg
    65641 tttactgcgg gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc
    65701 gtcaactcgc tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca
    65761 attaccggtc cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc
    65821 gctatactta acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga
    65881 aatatgggtc aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc
    65941 ggcggtaaat ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgcg
    66001 ccaggcccca ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatgtt
    66061 tcactcgcct gcccgtgggc gcaccgcacg ctgatcatgc gtaaactcaa aggactggaa
    66121 ccgtttattt ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat
    66181 gacagttttc cgggtgcaac cggcgataca ctctatcaac atgaattttt gtatcagctt
    66241 tatctccacg ccgatccaca ctacagcgga cgagttactg ttcccgtgct gtgggacaaa
    66301 aagaaccaca ccatcgtcag caacgaatca gcagaaatca tccgcatgtt caataccgcg
    66361 tttgatgcgc tgggcgcgaa agctggtgat tactacccat cagccctgca aacgaaaatt
    66421 gacgaactta acggctggat ttatgacacc gttaacaatg gcgtgtataa agccggtttt
    66481 gccaccagcc agcaagccta cgacgaggcg gtagtgaaag tgtttgaatc gctggcgcga
    66541 ctggagcaga ttttaggcca gcatcgttac ctgaccggca accagctaac cgaagccgat
    66601 attcgcctgt ggaccacgct ggtgcgtttt gatccggtgt atgtcaccca cttcaagtgt
    66661 gataagcacc gcatcagcga ttacctgaat ctgtatggtt tcctgcgcga tatctaccag
    66721 atgccgggga ttgccgaaac tgtcaatttc gatcacatcc gtaatcacta cttccgcagc
    66781 cataagacca tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa
    66841 ccgcatggac gagatgttcg cttcggttaa aaaaaggcac cttacgaggt gcctctgatt
    66901 attagctaag tgcgtgttat ttatgtcgga tgcggcgtga acgttttctc caacttacaa
    66961 aacgtttata ttcaatatgt tacaatgccg cgtaggccag ataagcgtag cgcatctggc
    67021 gattttgcgc ttgtcatcag tcttagcgcg ccttcacggc actttattaa ttccttgaac
    67081 gaatatttac tgccatttca ttcacgtcta ttcttaattt gctgctttaa aaacaagtga
    67141 ttgagcaaaa ttgaggcaaa aatggactgg tatctgaaag tactaaaaaa ttatgtcggt
    67201 ttccggggtc gtgcgcggcg caaagagtac tggatgttta ttctggtcaa catcatcttt
    67261 acgttcgtgc tggggctgct ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc
    67321 atcctgacaa caatctacgg tattctggtg tttttaccgt ggtgggcggt tcagttccgc
    67381 cgcctgcacg acaccgaccg ctcggcgtgg tgggcactgc tgttcttaat cccgtttatc
    67441 ggctggctta ttatcatcgt ctttaactgc caggcgggta cgccaggcga aaaccgcttt
    67501 ggacctgatc caaaactgga accttaaaac aaatctgctc gccggatgtc tgactcatcc
    67561 ggcttttatc
//
LOCUS       DAYPHN010000007        65420 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000007 DAYPHN010000000
VERSION     DAYPHN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.007"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(187..666)
                     /gene="folK"
                     /locus_tag="ACG0BW_000453"
     CDS             complement(187..666)
                     /gene="folK"
                     /locus_tag="ACG0BW_000453"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFK7257467.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTVLGNLLKINKFHELNKW"
     gene            complement(663..2060)
                     /gene="pcnB"
                     /locus_tag="ACG0BW_000454"
     CDS             complement(663..2060)
                     /gene="pcnB"
                     /locus_tag="ACG0BW_000454"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFK7257468.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYNLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     SRRPRKRAPRREGTA"
     gene            complement(2120..3016)
                     /gene="gluQRS"
                     /locus_tag="ACG0BW_000455"
     CDS             complement(2120..3016)
                     /gene="gluQRS"
                     /locus_tag="ACG0BW_000455"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFK7257469.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAADTILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3083..3538)
                     /gene="dksA"
                     /locus_tag="ACG0BW_000456"
     CDS             complement(3083..3538)
                     /gene="dksA"
                     /locus_tag="ACG0BW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFK7257470.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3716..4420)
                     /gene="sfsA"
                     /locus_tag="ACG0BW_000457"
     CDS             complement(3716..4420)
                     /gene="sfsA"
                     /locus_tag="ACG0BW_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFK7257471.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4435..4965)
                     /gene="thpR"
                     /locus_tag="ACG0BW_000458"
     CDS             complement(4435..4965)
                     /gene="thpR"
                     /locus_tag="ACG0BW_000458"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFK7257472.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAAHSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5039..7468
                     /gene="hrpB"
                     /locus_tag="ACG0BW_000459"
     CDS             5039..7468
                     /gene="hrpB"
                     /locus_tag="ACG0BW_000459"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFK7257473.1"
                     /translation="MSSLPVAAVLPELLAALDGAPQVLLTAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVIISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLQAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7562..10096
                     /gene="mrcB"
                     /locus_tag="ACG0BW_000460"
     CDS             7562..10096
                     /gene="mrcB"
                     /locus_tag="ACG0BW_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFK7257474.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10316..12574
                     /gene="fhuA"
                     /locus_tag="ACG0BW_000461"
     CDS             10316..12574
                     /gene="fhuA"
                     /locus_tag="ACG0BW_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFK7257475.1"
                     /translation="MARSKTAQPKHPLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEIQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGIARSANAQQKGAEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDDDALKASPCLSIPQSEWNHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVEHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPDPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWAEQSSFNRDYGNKSD
                     RDDKQFTWRGGVNYLFDNGVTPYFSYSESFEPASLTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVLTGALYQLTKTNNLMADPNNPNFSIEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12625..13422
                     /gene="fhuC"
                     /locus_tag="ACG0BW_000462"
     CDS             12625..13422
                     /gene="fhuC"
                     /locus_tag="ACG0BW_000462"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFK7257476.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13422..14312
                     /gene="fhuD"
                     /locus_tag="ACG0BW_000463"
     CDS             13422..14312
                     /gene="fhuD"
                     /locus_tag="ACG0BW_000463"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFK7257477.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQRAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14309..16291
                     /gene="fhuB"
                     /locus_tag="ACG0BW_000464"
     CDS             14309..16291
                     /gene="fhuB"
                     /locus_tag="ACG0BW_000464"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFK7257478.1"
                     /translation="MSKRIALFPALLLALLVIVAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALTLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLAGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMTLTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16326..17606)
                     /gene="hemL"
                     /locus_tag="ACG0BW_000465"
     CDS             complement(16326..17606)
                     /gene="hemL"
                     /locus_tag="ACG0BW_000465"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFK7257479.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17772..17819
                     /gene="yadX"
                     /locus_tag="ACG0BW_000466"
     CDS             17772..17819
                     /gene="yadX"
                     /locus_tag="ACG0BW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFK7257480.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17831..19252
                     /gene="clcA"
                     /locus_tag="ACG0BW_000467"
     CDS             17831..19252
                     /gene="clcA"
                     /locus_tag="ACG0BW_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFK7257481.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19276..19341
                     /gene="yadW"
                     /locus_tag="ACG0BW_000468"
     CDS             19276..19341
                     /gene="yadW"
                     /locus_tag="ACG0BW_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFK7257482.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19334..19678
                     /gene="erpA"
                     /locus_tag="ACG0BW_000469"
     CDS             19334..19678
                     /gene="erpA"
                     /locus_tag="ACG0BW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFK7257483.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19725..20348)
                     /gene="yadS"
                     /locus_tag="ACG0BW_000470"
     CDS             complement(19725..20348)
                     /gene="yadS"
                     /locus_tag="ACG0BW_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFK7257484.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20386..21186)
                     /gene="btuF"
                     /locus_tag="ACG0BW_000471"
     CDS             complement(20386..21186)
                     /gene="btuF"
                     /locus_tag="ACG0BW_000471"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFK7257485.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21179..21877)
                     /gene="mtnN"
                     /locus_tag="ACG0BW_000472"
     CDS             complement(21179..21877)
                     /gene="mtnN"
                     /locus_tag="ACG0BW_000472"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFK7257486.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21961..23478
                     /gene="dgt"
                     /locus_tag="ACG0BW_000473"
     CDS             21961..23478
                     /gene="dgt"
                     /locus_tag="ACG0BW_000473"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFK7257487.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23608..25032
                     /gene="degP"
                     /locus_tag="ACG0BW_000474"
     CDS             23608..25032
                     /gene="degP"
                     /locus_tag="ACG0BW_000474"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFK7257488.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25187..26344
                     /gene="cdaR"
                     /locus_tag="ACG0BW_000475"
     CDS             25187..26344
                     /gene="cdaR"
                     /locus_tag="ACG0BW_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFK7257489.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26398..26784)
                     /gene="yaeH"
                     /locus_tag="ACG0BW_000476"
     CDS             complement(26398..26784)
                     /gene="yaeH"
                     /locus_tag="ACG0BW_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFK7257490.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27097..27921)
                     /gene="dapD"
                     /locus_tag="ACG0BW_000477"
     CDS             complement(27097..27921)
                     /gene="dapD"
                     /locus_tag="ACG0BW_000477"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFK7257491.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27952..30624)
                     /gene="glnD"
                     /locus_tag="ACG0BW_000478"
     CDS             complement(27952..30624)
                     /gene="glnD"
                     /locus_tag="ACG0BW_000478"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFK7257492.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30686..31480)
                     /gene="map"
                     /locus_tag="ACG0BW_000479"
     CDS             complement(30686..31480)
                     /gene="map"
                     /locus_tag="ACG0BW_000479"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFK7257493.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31848..32573
                     /gene="rpsB"
                     /locus_tag="ACG0BW_000480"
     CDS             31848..32573
                     /gene="rpsB"
                     /locus_tag="ACG0BW_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFK7257494.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32831..33682
                     /gene="tsf"
                     /locus_tag="ACG0BW_000481"
     CDS             32831..33682
                     /gene="tsf"
                     /locus_tag="ACG0BW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFK7257495.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33829..34554
                     /gene="pyrH"
                     /locus_tag="ACG0BW_000482"
     CDS             33829..34554
                     /gene="pyrH"
                     /locus_tag="ACG0BW_000482"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFK7257496.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34846..35403
                     /gene="frr"
                     /locus_tag="ACG0BW_000483"
     CDS             34846..35403
                     /gene="frr"
                     /locus_tag="ACG0BW_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFK7257497.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35495..36691
                     /gene="ispC"
                     /locus_tag="ACG0BW_000484"
     CDS             35495..36691
                     /gene="ispC"
                     /locus_tag="ACG0BW_000484"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFK7257498.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36880..37638
                     /gene="ispU"
                     /locus_tag="ACG0BW_000485"
                     /gene_synonym="uppS"
     CDS             36880..37638
                     /gene="ispU"
                     /locus_tag="ACG0BW_000485"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFK7257499.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37651..38508
                     /gene="cdsA"
                     /locus_tag="ACG0BW_000486"
     CDS             37651..38508
                     /gene="cdsA"
                     /locus_tag="ACG0BW_000486"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFK7257500.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38520..39872
                     /gene="rseP"
                     /locus_tag="ACG0BW_000487"
     CDS             38520..39872
                     /gene="rseP"
                     /locus_tag="ACG0BW_000487"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFK7257501.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVLPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRQDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39902..42334
                     /gene="bamA"
                     /locus_tag="ACG0BW_000488"
     CDS             39902..42334
                     /gene="bamA"
                     /locus_tag="ACG0BW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFK7257502.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42456..42941
                     /gene="skp"
                     /locus_tag="ACG0BW_000489"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42456..42941
                     /gene="skp"
                     /locus_tag="ACG0BW_000489"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFK7257503.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42945..43970
                     /gene="lpxD"
                     /locus_tag="ACG0BW_000490"
     CDS             42945..43970
                     /gene="lpxD"
                     /locus_tag="ACG0BW_000490"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFK7257504.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44075..44530
                     /gene="fabZ"
                     /locus_tag="ACG0BW_000491"
     CDS             44075..44530
                     /gene="fabZ"
                     /locus_tag="ACG0BW_000491"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFK7257505.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44534..45322
                     /gene="lpxA"
                     /locus_tag="ACG0BW_000492"
     CDS             44534..45322
                     /gene="lpxA"
                     /locus_tag="ACG0BW_000492"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFK7257506.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45322..46470
                     /gene="lpxB"
                     /locus_tag="ACG0BW_000493"
     CDS             45322..46470
                     /gene="lpxB"
                     /locus_tag="ACG0BW_000493"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFK7257507.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGLGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46467..47063
                     /gene="rnhB"
                     /locus_tag="ACG0BW_000494"
     CDS             46467..47063
                     /gene="rnhB"
                     /locus_tag="ACG0BW_000494"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFK7257508.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47100..50582
                     /gene="dnaE"
                     /locus_tag="ACG0BW_000495"
     CDS             47100..50582
                     /gene="dnaE"
                     /locus_tag="ACG0BW_000495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFK7257509.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVMRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50595..51554
                     /gene="accA"
                     /locus_tag="ACG0BW_000496"
     CDS             50595..51554
                     /gene="accA"
                     /locus_tag="ACG0BW_000496"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFK7257510.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51653..53794
                     /gene="ldcC"
                     /locus_tag="ACG0BW_000497"
     CDS             51653..53794
                     /gene="ldcC"
                     /locus_tag="ACG0BW_000497"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFK7257511.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFDAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFSRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53851..54240
                     /gene="yaeR"
                     /locus_tag="ACG0BW_000498"
     CDS             53851..54240
                     /gene="yaeR"
                     /locus_tag="ACG0BW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFK7257512.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54305..55600
                     /gene="tilS"
                     /locus_tag="ACG0BW_000499"
     CDS             54305..55600
                     /gene="tilS"
                     /locus_tag="ACG0BW_000499"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFK7257513.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRAENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFMTQEGMAESENGVSFVWQRNG"
     gene            complement(55649..55909)
                     /gene="rof"
                     /locus_tag="ACG0BW_000500"
     CDS             complement(55649..55909)
                     /gene="rof"
                     /locus_tag="ACG0BW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFK7257514.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55896..56096)
                     /gene="yaeP"
                     /locus_tag="ACG0BW_000501"
     CDS             complement(55896..56096)
                     /gene="yaeP"
                     /locus_tag="ACG0BW_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFK7257515.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56262..56807
                     /gene="yaeQ"
                     /locus_tag="ACG0BW_000502"
     CDS             56262..56807
                     /gene="yaeQ"
                     /locus_tag="ACG0BW_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFK7257516.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWRRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56804..57226
                     /gene="arfB"
                     /locus_tag="ACG0BW_000503"
     CDS             56804..57226
                     /gene="arfB"
                     /locus_tag="ACG0BW_000503"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFK7257517.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57240..57950
                     /gene="nlpE"
                     /locus_tag="ACG0BW_000504"
                     /gene_synonym="cutF"
     CDS             57240..57950
                     /gene="nlpE"
                     /locus_tag="ACG0BW_000504"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFK7257518.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFYYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPMLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57980..58804)
                     /gene="yaeF"
                     /locus_tag="ACG0BW_000505"
     CDS             complement(57980..58804)
                     /gene="yaeF"
                     /locus_tag="ACG0BW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFK7257519.1"
                     /translation="MDKPKAHCCLLLPCFLFLSACTVDISQPDSSATAVDAGAKTWAV
                     QFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58857..60575)
                     /gene="proS"
                     /locus_tag="ACG0BW_000506"
     CDS             complement(58857..60575)
                     /gene="proS"
                     /locus_tag="ACG0BW_000506"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFK7257520.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60686..61393)
                     /gene="tsaA"
                     /locus_tag="ACG0BW_000507"
     CDS             complement(60686..61393)
                     /gene="tsaA"
                     /locus_tag="ACG0BW_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFK7257521.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNTRMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61390..61794)
                     /gene="rcsF"
                     /locus_tag="ACG0BW_000508"
     CDS             complement(61390..61794)
                     /gene="rcsF"
                     /locus_tag="ACG0BW_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFK7257522.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61912..62727)
                     /gene="metQ"
                     /locus_tag="ACG0BW_000509"
     CDS             complement(61912..62727)
                     /gene="metQ"
                     /locus_tag="ACG0BW_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFK7257523.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62767..63420)
                     /gene="metI"
                     /locus_tag="ACG0BW_000510"
     CDS             complement(62767..63420)
                     /gene="metI"
                     /locus_tag="ACG0BW_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFK7257524.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPIQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63413..64444)
                     /gene="metN"
                     /locus_tag="ACG0BW_000511"
     CDS             complement(63413..64444)
                     /gene="metN"
                     /locus_tag="ACG0BW_000511"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFK7257525.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64632..65204
                     /gene="gmhB"
                     /locus_tag="ACG0BW_000512"
     CDS             64632..65204
                     /gene="gmhB"
                     /locus_tag="ACG0BW_000512"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFK7257526.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15430 a        16273 c        18058 g        15659 t
ORIGIN      
        1 ttatatatcc acttaattca tctttaagta aaacttaatg tacaaagcgg gaaaaatatt
       61 gtatgtgagg cggaaggtgt taacggggta tataatatta attatggttg tagcttaggg
      121 aatatattaa ttgcaatggg gtaaggctat ttttcggaat ttatttatat tcatgaaatc
      181 attaaatcac catttgttta attcatgaaa tttatttatt tttaataaat ttcctaatac
      241 tgttccatca ggaaacacca gctccggcgc aatttcaaac agcggccaca gcataaatcc
      301 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatta cttcattacc
      361 aaatagcatg atgtcgagat ccagtgtgcg cggcccccag cgttccgctt tacggacgcg
      421 accttgttgt aactcaatac gttgtgtgtg gttgagtaac tcttcaggtg caagagaggt
      481 ttccagcgcc acggctgcgt ttaagtaatc gggttgatct tgcggcccca gcggtggagt
      541 gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg atatcgccta atgctttcag
      601 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      661 tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgaga acggcgacgc
      721 ggtgacggct cttcatccag ctcgttgagc atcccttttt gatctggtgg cgcggaaacc
      781 tggaactccc cccaccattt cacaagacgc tgcagttcag cgttacgttc aacttcagct
      841 cgcaaggcca acaggtcata agcagcacgg aacttaggat gctccagcag tttccaggcg
      901 cgtttaccct gacgtcggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      961 gtcagacgtt tcgggatcgc cagagaacgg caggcttcgt ccagcacgtc gttcatcgcc
     1021 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1081 agcagtgggt accagaacat ggcggcaaac aggaacgccg ggttcacgcg catatcgtta
     1141 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1201 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag gtgatattca
     1261 cacagcagat tataggtttc gtaaccgtag cccgcttgca gcagtttcaa agattcttca
     1321 aacagacgtg ccggtggaat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1381 gtttcaggac tgatacgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatccgt
     1441 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtctttc
     1501 agatccttca tgccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1561 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1621 cgcagcaaca taccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1681 tgatgtccac ggaaggtcgc aacttcgata atctccgggc caaacatcac atgagccaga
     1741 cggaaacggc gacccaccag gcggcagtta cggaacagtt tacgtacctg ttcaggcgtg
     1801 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1861 ccgccaacca gccaggcttc gtatcccgct ttattgagcc tgtacattac cttcagggca
     1921 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt
     1981 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     2041 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2101 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2161 actcaggcac tgcggtgagc cgccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2221 tgaaatcctg ccagtgtgtt tctacctgct gacccagaaa atgaagtgcc gcgattaaca
     2281 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2341 cttgtggatt aagcgccagc ggtaaatgga tgtaatctgg cactttccag ccaaaaagct
     2401 ggtacagcga gatttgccgc actgtcggtt cgatcaaatc tgccccccgg acaatttcgc
     2461 tcacgccctg gaagtgatca tccaccacca ccgccaggtt ataggcaaac aaaccatcac
     2521 ggcgatgaat aataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2581 gcaggtcagt aaactgggtg accggatgct gctggcggat acgtactgcg gcgttgtctg
     2641 gtccatgatg caattcccgg caatggccgt cgtaaatacc gccaatgctt tgaatacgcg
     2701 cacgcgtgca ggtgcagtaa taacttaatc cttgctcatg taaccaggcg agtgcttcgc
     2761 gataggcgtg gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc aggccgtaat
     2821 gttccagctg gcgcaggata gtatctgcgg caccgggaac ttcacgaggc gggtcgatat
     2881 cttcaatgcg taccagccag cgaccgtgcc gggcgcgagc ctgtaaatag ctgccaagcg
     2941 cggcaatcag agagccaaaa tgaagctcgc cggaaggaga gggggcaaag cggccaatat
     3001 agtgtgtgtc tgtcatctcg ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3061 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3121 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3181 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgttttct
     3241 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3301 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3361 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3421 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3481 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3541 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3601 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3661 gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta ctctactaca
     3721 atgtaaccgg tagtgatttt ttaagagcca tgccttcggc agaaagttcc gctttgtaag
     3781 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3841 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcaaaa aagataaccg
     3901 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3961 tgaccgcatc gggaaaatat ccctgttcgt tttccgctaa cgtaaccgat ttcacttcaa
     4021 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttctgcgc
     4081 cgtatttaac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4141 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata attgcgccgc
     4201 tctggctttg agtcaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4261 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4321 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4381 gaattagcgt cgcgcgctgt agagggggag aaaattccat tacgacaatc cttgttattg
     4441 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgcgcaa acgaggaggc
     4501 gtaaagggtg aactccgtca ccgtatacga ccagttaaaa cctggcggcg ggattgtcac
     4561 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4621 acaaccgctg tgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4681 cggctgacgc atccctaacc acaccacacg cgaacgcagc cattgaccgg cgtcatcaag
     4741 cgtgagtgtg aaacccggtt gacgaatccg cccggctaaa agagaaagcg ccttctcttt
     4801 ctcggcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4861 acgtcccgcg tcaggtggga agtgtgtggc gcgccagtgg ataatctgtt cgcggatttc
     4921 tgcaggtaag tcgatagcaa agaacagccg ttgcggttca gacatgtgag gcactcggtt
     4981 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     5041 gtcgtcgttg cccgttgctg ccgtcttacc tgagttactt gctgccctcg atggtgcacc
     5101 gcaggtgtta ttaactgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5161 gctggcgcat cccggcatta acgggaaaat tatcctcctg gagccgcgtc gtttggcggc
     5221 gcgtaacgtt gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5281 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5341 aggcgtgctg acgcgcatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5401 cctcgatgag tttcatgagc gcagcttgca ggcggatttg gcgttggcgc tgttactcga
     5461 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5521 cgaccgcttg cagcaaatgc tgccagaagc gcccgtcatc atctcagaag ggcgctcgtt
     5581 tccggttgaa cgccgttatt taccactgcc cacgcatcag cgttttgacg aagccgttgc
     5641 ggtagccacc gccgaaatgc tgcgtcagga aagcggatca ttactgttat ttttacctgg
     5701 cgtcggagaa attcagcgtg tgcaggaaca actggcttcg cgcatcggca gtgatgtgtt
     5761 gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga tccttccggc
     5821 accgcaagga atgcgcaaag tggtgctggc gaccaatatt gctgaaacaa gtttaaccat
     5881 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg
     5941 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     6001 cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat ttaatcgcca aagaacaagc
     6061 agaacgcgct gccgcgcaaa gtgaaccgga gatattacaa agcgatcttt ccggtttgct
     6121 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6181 gccagcagtg aatttacagg ccgcgaaacg cctgttacgg atgctggggg cgctggatgg
     6241 cgaacggcta agcgcgcaag ggcaaaaaat ggcggcgctg ggtaacgatc cgcgtttagc
     6301 ggcaatgctg gttagcgcga agagcgacga cgaagctgct accgcggcaa aaattgccgc
     6361 tattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6421 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgtttaaacg tacgcggcgg
     6481 tgaggcagac agttctctta tcgcgccgct acttgccggg gcgtatgccg atcgcattgc
     6541 tcgtcgccgt gggcaagatg gacgctatca actggcaaac ggcatggggg cgatgctcga
     6601 tgccgacgat gcgctaagcc gccacgaatg gttgatcgca ccgttattat tgcagggcag
     6661 cgcctcgccg gatgcgcgga ttttactggc gctgccggtc gatattgatg agttagtaca
     6721 acgctgcccg cagctggtac agcagtctga caccgtggaa tgggatgacg cgcaaggtac
     6781 gctgaaagcc tggcgtcggc tgcaaatcgg tcagttgacg gtgaaagtgc agccactggc
     6841 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaac ggcattcgtg ataaaggttt
     6901 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6961 aaagtggttg ccggaatatg actggccagc ggttgatgat gagagtttgt tggcgacgct
     7021 ggaaacgtgg ctactgccgc atatgagcgg cgtgcattca ctacgcggcc tgaaatcact
     7081 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7141 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7201 agataatccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccaccaatcc
     7261 gacgatcgcc caggggcgcg tgccgctggt gctggagttg ctttcccctg cccaaaggcc
     7321 gctgcaaatc acgcgtgatt tgagcgcctt ctggaaagga gcgtaccgtg aggtgcaaaa
     7381 agagatgaaa gggcgttatc ccaaacatgt ctggccggac gacccggcaa acacagcacc
     7441 aacgcgacga acgaaaaagt attcgtgaag atcaaagtaa atttgagaga tatcttcttc
     7501 tgtcttgcaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag
     7561 catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa
     7621 acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta
     7681 tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg
     7741 tggcaaacgc ggctggctat ggctgctgct aaaactggct atcgtttttg ccgtgctgat
     7801 cgccatttac ggcgtttatc tcgatcaaaa aattcgcagc cgtattgatg gcaaggtctg
     7861 gcaattgccc gcggcagttt atggccggat ggtcaacctt gagccagaca tgaccatcag
     7921 caagaacgag atggtgaagc tgctggaggc gacccagtat cgtcaggtgt cgaaaatgac
     7981 ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga
     8041 tttcccggac agcaaagaag ggcaggtgcg cgcgcgtctg acctttgatg gcgatcatct
     8101 ggcaacgatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg
     8161 tctgatcacc atgatctctt cgccaaacgg tgagcagcgt ctgtttgtgc cgcgcagtgg
     8221 tttccctgat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tctacgagca
     8281 tgatgggatc agtctctact ccatcggacg tgcggtgctg gcaaacctga cagccggacg
     8341 cacggtgcag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag
     8401 cgagcgctct tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg
     8461 ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg
     8521 cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga
     8581 agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta
     8641 caacccgtgg cgtaacccga aactggcgct ggagcgacgt aatctggtac tgcgtctgct
     8701 gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgcac gtccgctggg
     8761 cgttcagccg cgcggtgggg taatctctcc acagccagcc tttatgcagc tggtgcgtca
     8821 ggagctgcag gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac
     8881 tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc
     8941 actgaagaaa cagcgtaagt tgagcgatct tgaaaccgcg attgtggttg ttgaccgctt
     9001 tagcggtgaa gttcgtgcga tggtcggtgg ttctgagccg cagtttgcgg gctacaaccg
     9061 tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc
     9121 cttaagccag ccgaaaatct atcgtttgaa tacgtggatt gccgatgcgc caatcgcgct
     9181 gcgtcagccg aatggccagg tctggtcacc gcagaatgat gaccgtcgtt atagcgaaag
     9241 tggcagagtg atgctggtgg atgcgttgac ccgttcgatg aacgtgccga cggtgaatct
     9301 ggggatggcg ctggggctgc ctgcggttac ggagacctgg attaaactgg gcgtaccgaa
     9361 agatcagctg catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga
     9421 agtggcgcag gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact
     9481 gcgttcggta atcgcggaag atggcaaggt gctgtatcag agcttcccgc aggcggaacg
     9541 cgcggttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg
     9601 tggtacgggg cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg
     9661 gactaccaac aataacgtag atacctggtt tgcgggcatt gacggcagca cggtgaccat
     9721 cacctgggta ggccgtgata acaaccagcc gaccaagctg tatggtgcca gcggggcaat
     9781 gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc
     9841 agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat
     9901 gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gtgagatgca
     9961 gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca
    10021 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat
    10081 gtttggtagt aattaacgtc taagcgtgac ataccggatg gcgagtagcc atccggtaaa
    10141 ataacatccc acccaaagta ttaacccttt cttttcatct ggttgtttat taacccttca
    10201 ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct
    10261 ttataataat cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc
    10321 gcgttccaaa actgctcagc caaaacaccc actgcgtaaa atcgcagttg tagtagccac
    10381 agcggttagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
    10441 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggccagctg caactattgc
    10501 ggcgcgacag tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc
    10561 tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc
    10621 tcttagctac actcctggcg ttgccgtggg aactcgtggc gcatccaaca cttacgatta
    10681 cctgatcatc cgcggattcg ccgctgacgg ccaaagccag aacaactatc tgaatggcct
    10741 gaagatgcag ggcaacttct ataacgatgc agttatcgat ccgtatatgc tggagcgcgc
    10801 cgaaatcatg cgtggtccgg tatccgtgct gtacgggaaa agcagccctg gcggcctgtt
    10861 gaacatggtc agcaagcgcc cgactacaga accgctgaaa gaaattcagt ttaaagccgg
    10921 tactgacagc ctgttccaga ctggttttga tttcagtgat gcgctggatg atgacggcgt
    10981 ttactcttat cgtctgactg gtattgcgcg ttcagccaat gctcagcaga agggggcaga
    11041 agagcagcgt tatgctattg ctccagcgtt cacctggcgc ccggatgata aaaccaattt
    11101 cactttcctt tcttacttcc agaacgaacc ggaaaccggt tattacggct ggttgccgaa
    11161 agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg
    11221 ggcgaagaac aacacctatt cacgtaatga gaagatggtg ggctacagct tcgatcacga
    11281 atttaacgac acctttactg tgcgtcagaa cctgcgcttt gcgcaaaata aagtctcgca
    11341 aaagagcgta tatggctacg gcatgtgctc ggatccgctg tataccaaag acgacgatgc
    11401 actcaaggcg agtccatgtt taagtatccc gcagtcagaa tggaatcata cactgactcg
    11461 tcagtacgtt atcgataatg agaaattaga aaacttctct gttgatactc agttgcaaag
    11521 taaattcgca accggctcgg ttgaacacac tctgctgacc ggcgttgact ttatgcgtat
    11581 gcgtaatgat attgactcct ggttcggtta cgccggttcc gttgcaccgt ctgatatcta
    11641 taatttagac cgtagtgact ttgattttgg tgctcacccg gatccgtcgg gcccataccg
    11701 cgttttgctt aaacagaaac aaaccggtct gtatgttcag gatcaggcgc agtgggataa
    11761 ggtgctggtg actctgggtg gtcgctatga ctgggcggaa cagtcatctt tcaaccgtga
    11821 ttacggtaat aaatccgatc gtgatgacaa acagttcacc tggcgtggtg gcgtaaacta
    11881 cctgttcgac aacggggtaa cgccttactt cagctacagt gagtcgtttg agccagcatc
    11941 tttgacagat gcaaacggtg atctgtttgc accttcgaaa ggcaaacagt atgaagttgg
    12001 tgtgaaatat gtgccggaag atcgcccaat tgtgctgacg ggcgcactgt atcagcttac
    12061 caaaaccaac aacctgatgg cggatccgaa taatcccaat ttctcgattg aaggcggtga
    12121 gattcgcgct cgtggtgtag aactggaagc aaaagcggcg ctgtcggcga gtgttaacgt
    12181 ggttggttct tatacctata ccgatgcgga atacactacc gatactacct acaaaggcaa
    12241 tacgcctgca caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga
    12301 cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg
    12361 tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta
    12421 tgatctggca cgagtcggca tggctggctc caacgtggcg ctgcatgtca acaacctgtt
    12481 cgatcgtgaa tacgtcgcca gctgctttaa cacctatggc tgcttctggg gcgcagaacg
    12541 tcaggtcgtt gcaaccgcaa ccttccgttt ctaatttctc ttttggggca cggatttccg
    12601 tgcccgtttc acaagttggc tgttatgcag gaatacacga atcattccga taccactttt
    12661 gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta
    12721 acctttcctg ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact
    12781 ctgctcaaaa tgcttggtcg tcaccagcca ccgtcggaag gggagattct tcttgatgcc
    12841 caaccgctgg aaagctggag cagcaaagcg tttgcccgca aagtggctta tttgccgcag
    12901 cagcttcctc cggcagaagg gatgacggtg cgtgaactgg tggcgattgg tcgttacccg
    12961 tggcatggcg cactagggcg ctttggtgcg gcagatcgcg aaaaggtcga ggaagctatc
    13021 tcgctggttg gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa
    13081 cgtcagcggg cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac
    13141 gaaccgacct cggcgctgga tatcgcccac caggttgacg tgctggcgct ggtgcatcgt
    13201 ttaagtcagg aacgtggtct gacggtcatt gccgtgctgc acgatattaa tatggcggcg
    13261 cgctactgtg attatctggt cgccctgcgc ggcggtgaaa tgattgctca gggaacgcct
    13321 gcggaaatta tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattctg
    13381 ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta
    13441 tttcgcgccg tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata
    13501 ctgcccacgc ggcggctatt gatcccaatc gtattgtggc gctggagtgg ttgccggtgg
    13561 aattactgct ggcgctcggc atcgtgcctt acggcgtggc ggataccatc aactatcgcc
    13621 tgtgggtcag cgaaccacca ttgccggact cagtgatcga cgtcggtttg cgcacagaac
    13681 ctaaccttga actgctgacc gaaatgaaac catcgtttat ggtctggtcg gcaggatatg
    13741 gcccttcacc agaaatgctg gcacgtattg cgccgggtcg cggatttaac ttcagtgacg
    13801 gcaaacagcc gctggcgatg gcgcgtaaat cgctgacgga aatggcagat ttacttaacc
    13861 tgcaaagggc agcggaaaca catttagcgc aatatgaaga ctttatccgc agcatgaaac
    13921 cccgctttgt gaagcgtgga gcgcgcccgt tattgctgac gacgcttatc gatccgcgcc
    13981 atatgctggt cttcggtcca aacagcttgt tccaggaaat tcttgatgag tacggcatcc
    14041 caaatgcctg gcaaggggaa accaacttct ggggcagtac cgccgtcagt attgatcgtc
    14101 tggcagcgta taaagacgtt gatgtgctct gctttgatca cgacaacagc aaagacatgg
    14161 acgccctaat ggcgacgccg ctgtggcagg ctatgccgtt tgtccgcgcc ggacgctttc
    14221 agcgcgtacc tgcagtctgg ttttatggtg cgacgctctc ggcaatgcac tttgtgcgta
    14281 ttctggataa cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt tcccggcgtt
    14341 attgctggcg ctgctggtga ttgtcgcagc ggcgctcacc tggatgaact tttcgcaagc
    14401 gctgccgcgc agccagtggg cgcaggccgc ctggtcgccg gatattgacg tcatcgagca
    14461 gatgattttt cactacagct tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg
    14521 cctggggctg gtgggcgtgc tgtttcagca agtgctgcgt aacccgctgg cggaaccgac
    14581 gacgttgggc gttgcaacag gcgcgcaact ggggattacc gtcactacgc tctgggcgat
    14641 cccaggtgct atggcgagcc agtttgctgc gctggcaggg gcttgcgttg ttggcttaat
    14701 tgtctttggc gtcgcgtggg ggaaacggct gtcgccggta acgctgatcc tcgcggggct
    14761 ggtagtgagc ctttattgtg gggcaatcaa tcagttactg gttatcttcc atcatgacca
    14821 gttgcaaagc atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggcgt
    14881 tgagcgttta tggccgcagt tgctgggcgg cgtgatgctg acgttactgc tacttcgtcc
    14941 gttaaccctg atggggcttg atgatggcgt ggcgcgcaac ctcgggctgg ccttgtcgct
    15001 cgcgcgtctg gcggcgctga cgctggcgat agtcatcagt gcgctgctgg tgaatgcggt
    15061 ggggattatc ggctttatcg ggttgttcgc gccgctgctg gcaaaaatgc tgggggcgcg
    15121 gcgtttgttg ccacgattga tgctggcgtc gctgattggg gcgttaattc tgtggctttc
    15181 tgatcaaatc atcctctggc tgactcgcgt gtggatggaa gtttctaccg gttcggtcac
    15241 tgcgttgata ggtgcgccgc tgctactttg gctgctgccg cgtttacgca gcattagcgc
    15301 gccggatatg aaggtcaacg atcgtgtcgc tgctgaacgc caacatgtgc tggcgtttgc
    15361 cctcgcgggc ggcgtgctgc tgttgatggc agtggtggtg gcgctgtcgt ttggccgtga
    15421 tgcgcacggc tggacgtggg cgagcggggc gttgctcgag gatttgatgc cctggcgctg
    15481 gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat
    15541 tcagcggctg gccggcaacc cgatggcaag cccggaagtg ctggggatta gctccggcgc
    15601 ggcgtttggc gtggtgttga tgctgtttct ggtgccgggt aatgcctttg gctggctgtt
    15661 acctgcaggc agtctcggcg cggcggtgac gctgttgatc attatgatcg ccgccggccg
    15721 cggtggattt tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcgttcac
    15781 catgcttttg atgatgttgc aggcaagtgg tgacccgcga atggcgcaag tgctgacctg
    15841 gatttccggt tcgacctaca acgcgaccga tgcgcaggtc tggcgtaccg gaattgtgat
    15901 ggtgattttg ctggcgatta ccccgctgtg ccgccgctgg ctgaccattt taccgctggg
    15961 aggtgatacc gcacgagccg tggggatgac gctgacgccg acgcgaattg cgctgctgct
    16021 gttagcggct tgcctgacag cgactgcgac aatgaccatt ggaccgttga gttttgttgg
    16081 tttaatggca ccgcatattg cgcggatgat gggctttcga cggacgatgc cacacatcgt
    16141 aatttcggcg ctggtgggag gattactgct ggtgttcgct gactggtgtg ggcggatggt
    16201 gctgtttcca ttccagatcc cagcggggct gctgtcgacc tttatcggcg cgccatattt
    16261 tatctatttg ttgagaaagc agagccgtta attgaatgcc tcgcccggtg ggcgaggcaa
    16321 aatcatcaca acttcgcaaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc
    16381 atgctgtgcg ccacagacat aaagcccgct tcaaacgctg acggtgccag ataaacacct
    16441 tcgtccagca tcatatggaa gaaacgctta aagcgttcca cgtcacaggc catcacatcc
    16501 tgatagcacg tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg
    16561 tttaccacca gcggaattcc ggcttcttct gccgcttcca gcagaccttc tgccagacgt
    16621 gttgtcagct catccagcgt ttcgtgaacg cccggctgcg cgacttcatt cagacaggcg
    16681 aaaccagcag ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggaccg
    16741 gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca
    16801 ccgccgatga ttttgccgag gcaggtgaga tccggttcca caccgtaata atcctgtgcg
    16861 ccagctagcg ctacgcggaa accggtcatc acttcatcga tgatcagcaa cgcgccaaat
    16921 tcgtcgcaca gcgcgcgcag acctggcagg aactctggca gcggtggaac gcagttcata
    16981 ttgcctgcca ccggctcgac gataatacag gcaatctctt gcgggtattg ctcaaacgcg
    17041 gcgcgtacag aggccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc
    17101 ggaacgcccg gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc
    17161 aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa taattttatc gcgaccggta
    17221 aaaccacggg ccaggcggat ggcgctcatt gtcgcttcag tgccggagtt caccatgcgc
    17281 accatatcca tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg
    17341 gttggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggatcgcc
    17401 ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg
    17461 ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc
    17521 acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg
    17581 ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc
    17641 tgctattcta tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact
    17701 ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc
    17761 aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag
    17821 tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg
    17881 acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt
    17941 tatggcggca gtcgtcggca cgcttgtcgg gctggctgcg gttgcttttg acaaaggtgt
    18001 cgcctggttg cagaaccagc gtatgggggc gctggtacat actgctgata attatccgct
    18061 tctgttaact gtcgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt
    18121 gcgcaaatac gcgccggaag caggtggttc ggggatcccg gaaattgaag gggcgctgga
    18181 agatcaacgt cccgttcgct ggtggcgtgt gttgccggtg aaattctttg gcgggctggg
    18241 aacactcggc ggaggcatgg tgctggggcg cgaagggcca accgtacaga tcggcggcaa
    18301 tatcggtcgc atggtgcttg atattttccg cctgaaaggt gatgaagcgc gccatacgct
    18361 gctggcaacc ggtgctgcgg cggggctggc tgcggccttt aacgccccgc tggcgggtat
    18421 tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc
    18481 ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc
    18541 gttgattgac gtcggtaaac tttctgatgc gccgcttaat acgctgtggc tttatctgat
    18601 cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca
    18661 ggatttgctg caccgtgtgc acggtggcaa tattaccaaa tgggtactga tgggcggtgc
    18721 gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt
    18781 taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt
    18841 cgtcgctcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt
    18901 tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga
    18961 gctgtttccg caataccacc ttgaggcggg gacgtttgct attgccggaa tgggggcatt
    19021 actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga
    19081 taactaccag ctcattttgc ctatgattat taccggtctt ggcgcaacac tattagcgca
    19141 atttaccggc gggaaaccgc tatactcggc gattcttgcg cgcacgctgg caaaacagga
    19201 agctgagcaa ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata
    19261 ccagggtatt agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg
    19321 tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc
    19381 caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta
    19441 tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa
    19501 cgaaggcgat atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct
    19561 gcaatatctg gtcggcggtt ccgttgacta taccgaaggt ctggaaggtt ctcgtttcat
    19621 cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc
    19681 tgttgttcat gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca
    19741 tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc
    19801 gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc
    19861 gtagcgtgga caataccgcc gataatacag gctgttgcgt agatttctgt acgtaaaatc
    19921 atggggattt cgcgggccag tacatcacga atgatcccgc cgccaacgcc agtaatgacg
    19981 cccatacaaa ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg
    20041 ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt
    20101 cttggctggc gcaccagcac gatggtcagc atgctggtta ccattgcaac gaccagatcg
    20161 gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgttccg
    20221 ccgcctactg cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt
    20281 ccggctaaca aaacgccaga gatagcaaat accgctgtgc cgactatatc cagccaatag
    20341 acgagcattg ttcaatcccc actgagcacc tgaaaaggtc aggcgctaat ctacctgtga
    20401 aagcgcatta cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca
    20461 gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt
    20521 aggaatttgg tccggtccgc ctgtaatgac tatcgcctgt ggcgcgcgtg ctaacacctg
    20581 ttcgcggcta acttgcggcc agggaacccg gctatcttta aagatgtttt ctccgccaca
    20641 aacttcgagt acctggttct gaatcgactc ttttccactg gtaaatggcg gattaatgcc
    20701 gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta
    20761 ctggtccagc agggattgcg cggcttgttc ggccttgtct ggttgcggac tccagggggc
    20821 cagttgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt
    20881 tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat
    20941 caccagatcg ggtttcagcg ccacaatgcg ttccagattc atcccctgcc aggtggaaac
    21001 ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt
    21061 gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc
    21121 ggcgttgagc cacagtggcg caagaaaaga cagggcgacc agcgccctga acagtgactt
    21181 agccatgtgc cagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa
    21241 cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc
    21301 gtactacgac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca
    21361 tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt
    21421 tgatgaatgc gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa
    21481 tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg
    21541 gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgtgcttcgt
    21601 ccgagacaac gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt
    21661 taataatcac atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga
    21721 ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga
    21781 tttcgcaacc gccgagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa
    21841 cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga
    21901 tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac
    21961 atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccacag
    22021 ggcgttaaaa ccgaacatga gatcctgcga atcttcgaga gcgatcgcgg gcgtatcatc
    22081 aactctccgg caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat
    22141 gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc
    22201 gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata cggtctggat
    22261 gaactgaccg gaccttttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc
    22321 aatccgccgt ttggtcattt tggcgaagca gcgataaatg actggtttcg ccaacgttta
    22381 cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcagcacta
    22441 cgtttacggg acggggaaga accgcttaac gagctgcggc gcaaaatacg tcaggactta
    22501 tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat
    22561 ctcacctggg cacaggttgg cggtatttta aaatatactc gtccggcgtg gtggcgtggt
    22621 gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaggaa
    22681 gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta
    22741 acgtggatta tggaagctgc cgacgacatc tcctattgtg tggcagatct tgaagatgcg
    22801 gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga ggcgtggggc
    22861 cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc
    22921 tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc
    22981 ctaaataaac tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc
    23041 gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttctaaag
    23101 ttatataaaa atgtcgctgt aaaacatgtg tttagccatc cagatgtcga gcagcttgaa
    23161 ttgcagggct atcgggtcat tagcggatta ttagagattt atcgcccttt attaagcctg
    23221 tcgttatcag actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgaa
    23281 tcgcgcttat tcaacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt
    23341 aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg
    23401 ctgcaggatt atatcagcgg tatgaccgac ctctatgcct gggatgaata ccgacgtctg
    23461 atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc
    23521 agaaacttta gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc
    23581 gttatctgtt aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg
    23641 gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca
    23701 gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca
    23761 gtggtcagca ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat
    23821 ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc
    23881 tctccgttct gccagggggg ccagggcggt aatggcggcg gccagcaaca gaaattcatg
    23941 gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac
    24001 gttgttgata acgcgacggt gattaaagtc cagctgagcg atggtcgtaa gttcgacgcg
    24061 aagatggttg gcaaagatcc gcgctctgat atcgcgctga tccagatcca gaacccgaaa
    24121 aatctgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta
    24181 gcaattggta acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg
    24241 gggcgtagcg gcctgaatgc tgaaaactac gaaaacttca tccagaccga tgcagcgatc
    24301 aaccgtggta actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac
    24361 accgcgatcc tcgcaccgga cggcggcaac atcggtatcg gttttgctat cccgagtaac
    24421 atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg
    24481 ggtattatgg ggactgagct gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag
    24541 cgcggtgctt tcgtaagcca ggttctgcct aactcctccg ctgcaaaagc gggcattaaa
    24601 gcgggtgatg tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt
    24661 gctcaggtgg gtactatgcc ggtgggcagc aaactgaccc tgggcttact gcgcgacggt
    24721 aagcaggtta acgtgaacct ggaactgcag cagagcagcc agaatcaggt tgattccagc
    24781 tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg
    24841 gtagtgaaca acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa aaaaggtgat
    24901 gtgattattg gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc
    24961 gacagcaaac cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg
    25021 ttaatgcagt aatctccctc aaccccttcc tgaatacggg aaggggttct ccttacaatc
    25081 tgtgaacttc gccacaactc catacatctt catcatcctt tgggcatttg cacaatgccg
    25141 tacgttacgt acttccttat gctaagccgt gcataatgga ggacttatgg ctggctggca
    25201 tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgatactaa
    25261 tatcaacgta atggatgccc gcgggcgaat tatcggcagc ggcgatcgtg agcgtattgg
    25321 tgaattgcac gaaggagcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga
    25381 cgcggtggca cgtcatctgc acggtgtgcg gcaggggatt aatctgccgt tacggctgga
    25441 aggtgaaatt gtcggcgtta ttggcctgac tggtgaacca gagaacctgc gtaaatatgg
    25501 cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacctgtt
    25561 ggcgcaggat agccgtttgc gggaagaact ggtgatgaac ctgattcagg ctgaggagaa
    25621 tactcccgca cttacggaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt
    25681 ggtggctatt gttgaggtcg acagcggtca gcttggcgtg gacagcgcaa tggcggagtt
    25741 acagcaactg caaaacgcgc tgactacgcc tgagcgtaat aatctggtgg caattgtttc
    25801 gctgaccgaa atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgcaga
    25861 agatcatcgt aagcgagttg aacaactgat tacccgcatg aaagagtacg gccagctgcg
    25921 ttttcgcgtt tcgctgggta actattttac cggaccgggc agtattgccc gctcttatcg
    25981 tacggcgaaa acgacgatgg tggtgggtaa acagcgaatg ccagaaagtc gctgctattt
    26041 ttatcaggat ctgatgttgc ctgtgttact cgacagtttg cgtggcgact ggcaggccaa
    26101 cgaactggcg cgaccgctgg cgcggctgaa agcgatggac aataacggct tgctgcgacg
    26161 aacgctggcg gcgtggtttc gccacaatgt gcaaccgctg gctacgtcaa aggcgctgtt
    26221 tattcatcgt aatactctgg agtatcggct taatcgtata tcggaactga ccgggctgga
    26281 tttgggcaat tttgatgaca ggttgctgct gtatgtcgcg ttgcaactgg atgaagagcg
    26341 gtaggtcgat tacaaaatta cagccgggtg agactttacc cggcccacag aatggcatta
    26401 tttacgcgtt agtttttcca gatcggcttc aatctcgctg atcttattgg ttacaactga
    26461 ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg
    26521 ctggcagatt tgatctaact catcaatgat atagcgaaga ttagggctaa tctcctgtac
    26581 ttctttataa ccctgaccca caccatcagc gaccaccgtt ttacgctgac gcggatattt
    26641 aaacttcacg ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat
    26701 atcgttgttg gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc
    26761 cagacttttc agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta
    26821 ttttagcatt tggcctggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc
    26881 agacttctat catggcgtaa aaatcaaaaa attacctgct ttattgtggc gatattccat
    26941 gggatggatt agcacattta tctttgtttt gcgaattaca tgacagtccg aaacaagctg
    27001 aaaaagtatt taaaaaaggg gaagaagaat gagtaggacg aattattctg aaccagaaag
    27061 caatcgggca gcgtcgtgct gcccgtgtgc atacttttag tcgatggtac gcagcagttc
    27121 gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa ccgcacagta
    27181 aagactgtat ttgccatctt tcgacggcag attgcctgaa acaaccacag atcccgccgg
    27241 aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc tctgaccaat
    27301 gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa ccacttcaga
    27361 gcgcgcgccg atgaagcagt tatcttcaat aatggtcggg ttagcctgca gcggctccag
    27421 cacgccgccg atacccacgc caccggaaag gtggacgttt ttaccaatct gcgcacagga
    27481 accgacggtc gcccaggtat caaccatcgt gccttcatca acatatgcgc cgatgttgac
    27541 gtaagacggc atcagcacgg tgttacgggc aatgaacgcg ccctgacgca ctgccgctgg
    27601 tggcacgacg cggaagcctt ctttctggaa acgtgcttca tcgtagttag cgaatttcat
    27661 cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat cattaatacg
    27721 gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac cgtcaatttt
    27781 ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta ccgcttcgcg
    27841 ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag cggtttcaat
    27901 aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac atcacccttt
    27961 atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca actcgttatt
    28021 aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct cgccaatggt
    28081 tgtaattcgg gcgccatgaa gcgaaattcc cagatcggca aaaattttcc cgactctcgc
    28141 cagcagtcct ggctggtcga gcgcgataag ctcaaggaac gatttgcggt cggtatgggt
    28201 tggcaaaaac gtcacttcgg tttcaacagt aaagtgacgt agtttagccg gttgacgacg
    28261 cggctgtggt ggctgccagc tactttgtgt cagtacttgc tccagaccaa accgaataac
    28321 ctcatgacga tctgcggata gcgggctgcc atcgggttcc agcacgatga aggtatccat
    28381 cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat tgcggcggtc
    28441 taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga taaaaatctc
    28501 ggtgcctcca cgcgtagcct gcggactaag caataccagc ggtttgctta aatcatgctg
    28561 taataaatgg cgcgcatgcc aggccagttg atttgggcta tggcggacaa aatagttagc
    28621 acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca tgcgcagtag
    28681 tgccagcgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg tgttttgcat
    28741 tccgcgtcgt agctgctttt cggtggcgaa atagagttcg cgcaacagac tttgcttcca
    28801 gctattccac agcgtttcgt tggtggcgca tatatcggcc actgtcaggc ataccagata
    28861 acgaagtcgg ttttctgttt gcacttcttc ggcaaactgc ttgatcactt ccgggtcctg
    28921 aatatcgcgg cgttgggcgg tcaccgacat caacaggtgc tggcgaacca gccacgccac
    28981 cagctgtgtt tcgcgcgagt taagcccgtg gagttcggca aaatgcacca cgtcttgtgc
    29041 accgagaatg gagtggtcgc cgccacgccc tttggcgata tcgtgaaaca gcgcggcgat
    29101 gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg gatggcgctg
    29161 gcgtgtttct tcactggcaa aactctccag tttcagcatc acgcgaatag tatgttcatc
    29221 caccgtgtag gcgtggaaca ggtcaaactg catctgcccg acgatatgcg accattgggg
    29281 catatacgcg ccgagcacgc tatgacgatg catcggtaat agtccgcgac gcaccgcacc
    29341 agggtgacgc agaatgctca aaaacagttt tcgcgcttcc ggaatattac acagcggttg
    29401 ttgcaaatgg cgacgggcgt ggcgtaactg gcgcagcgtg gtagagtaaa tgccagtgat
    29461 cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct ggcgcataaa
    29521 tagtgtttca tcacgcaggt cgatcagcgt accgcgtagc tgaaactcat cgtcgattgg
    29581 acgtggtttt tcgtcggcgg gcagggcgag gatggcttca tcgaacagtt gcagcagcat
    29641 ctggttgagt tcgctgacgc ggcgtgtaac gcggaagtaa tccttcatca tccgctcgac
    29701 cggttcgtta ccttcgccac tgtaattcaa acgctgggcg acgctaagct ggcgatcgaa
    29761 taacaggcga ttatcgtaac ggctgacgac aagatgcagg gcaaagcgaa tacgccacaa
    29821 tatatgcaga cattcgttta attccgctcg ctccgctgag gttaagaagc caaacccgac
    29881 catttcatcc aacgatgttg cgccaaaatg acggcgggcc acccattgca gcgtgtggat
    29941 atcgcgcaag ccgccggggc tacttttgat gtctggctca aggttgtagc tggtgccgtg
    30001 gtaacgctga tggcgttggt tctgttcttc aactttcgct gcgtagaact tgtcggaagg
    30061 ccagaatcct tcgctgaaaa tatgtttttg cagttcgagg aacagcgcaa catcgccaat
    30121 taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc cttccagcat
    30181 gcactcttca agcgtgcgca cgctatgacc gacttccagc ttcacatccc agagcagcgt
    30241 taacagctcg cccacttttt gcgcctgatc atccgggagc tttttacggc ttaaaatcag
    30301 taaatcgatg tcggaaagtg gatgcagctc gccacgacca taaccaccga cggcaaccaa
    30361 tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca ggagctggtc
    30421 tatgaactcg gtacgtgcct caatcaactg ttctgcggag atcccattgt caaaggcatc
    30481 acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg ttaattcatc
    30541 acggggccag gcgcatggat tttgcggttg accgggcagg gtggggagag cggtgtttgc
    30601 gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta tcgcattaaa
    30661 aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc gggatggtgt
    30721 catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata gtatgctcgt
    30781 attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc atggtgcgga
    30841 tctctttttt acccgcgttg accattggct cgatggtgaa ggtcatcccc ggtttcagca
    30901 cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg aagccgcgac
    30961 caataccgtg tccacaatat tcacgaacga cggagaagcc ttctgcttcg acaaacttct
    31021 gaattgccgc accgatttcg cgcagattaa tgccaggttt taccatgcgc agcgccaggt
    31081 acaggctttc ttgcgtgatg cggcacagac gttcgcccat gatggtcggc ttaccgacga
    31141 taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca tcaatgttaa
    31201 cgatatcgcc atctttcagc agcttggcgt cgtccgggat accgtggcac accacttcat
    31261 taatagaaat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca gaaaccgcgt
    31321 gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg ctgacgcccg
    31381 gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca gcgacgcgca
    31441 ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc catcagcgtc
    31501 ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg gcaaattgag
    31561 aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga aaattcaacg
    31621 tctgatgctg tacacagcgc caacaattat tggtgtccgc gacgtatttg tggtataaag
    31681 cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac aatctcactt
    31741 tgtgtaacaa cacacacgta tcgacacata ttccggggtg ccctttgggg tcggtaatat
    31801 gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg gcaactgttt
    31861 ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt tactggaacc
    31921 cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc aaccttgaga
    31981 aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgct tctcgcaaag
    32041 gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa gacgctgctc
    32101 tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg actaactgga
    32161 aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct caggacggta
    32221 ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg gagaaactgg
    32281 aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg tttgtaatcg
    32341 atgctgacca cgaacacatt gctatcaaag aagcaaacaa cctgggtatt ccggtatttg
    32401 ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg ggtaacgacg
    32461 acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta cgtgaaggcc
    32521 gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag taataaggct
    32581 tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag aattcccggg
    32641 agcttacatc agtaagtgac cgggatgagc gagcgaagat aacgcatctg cggcgcgaaa
    32701 tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg ggcctgcata
    32761 tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc acatctccga
    32821 ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg
    32881 cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca tcgagctggc
    32941 aatcgaaaac atgcgtaagt ctggtgctat taaagcagcg aaaaaagcag gcaacgttgc
    33001 tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc tggaagttaa
    33061 ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag acaaagttct
    33121 ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga
    33181 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc
    33241 gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggtg ttctggttgc
    33301 tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg ctgcaagcaa
    33361 gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag aataccaggt
    33421 acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga aaatggttga
    33481 aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga
    33541 accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat
    33601 ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc
    33661 tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc ctgagggcgg cttctttttg
    33721 tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg
    33781 tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat ggctaccaat
    33841 gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct gcagggcact
    33901 gaaggcttcg gtattgatgc aagcatactg gatcgcatgg ctcaggaaat caaagaactg
    33961 gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct
    34021 ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat gctggcgacc
    34081 gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg
    34141 atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga agctatcagc
    34201 ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc gttctttacc
    34261 accgactcag cagcgtgcct gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca
    34321 accaaagttg acggtgtgtt tactgctgat ccggcgaaag atccaactgc aaccatgtac
    34381 gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga cctggcggcc
    34441 ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaacatgaa caaaccgggt
    34501 gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga ataattcccg
    34561 tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag tctggttata
    34621 aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg agcgtacaca
    34681 agtacgtgag gatggcgagc actgcccggg gccaaaatgg caaataaaat agcctaataa
    34741 tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact tccactgtgt
    34801 gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat tagcgatatc
    34861 agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac ccaaatcagc
    34921 aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt ggaatattac
    34981 ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc ccgtactctg
    35041 aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat tatggcgtcc
    35101 gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct gccgccgctg
    35161 acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga acaagcgcgt
    35221 gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag tgaaagcact gttgaaagat
    35281 aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa actgactgat
    35341 gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact gatgcagttc
    35401 tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc tggcggcgtt
    35461 ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca ttctgggctc
    35521 gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg aacacttccg
    35581 cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt gcctggaatt
    35641 ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta aaacgatgct
    35701 acagcaacag ggtagccgca ccgaagtctt aagtgggcaa caagccgctt gcgatatggc
    35761 agcgcttgag gatgttgatc aggtgatggc agccattgtt ggcgctgctg ggctgttacc
    35821 tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag aatcactggt
    35881 tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat tgttaccggt
    35941 cgatagcgaa cataacgcca tttttcagag tttaccgcaa cctatccagc ataatctggg
    36001 atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt ctggtggccc
    36061 tttccgtgag acgccattgc gcgatttggc aacaatgacg ccggatcaag cctgccgtca
    36121 tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg gctaccatga tgaacaaagg
    36181 tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga tggaagtgct
    36241 gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca gtgttctggc
    36301 gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat ggccgaatcg
    36361 cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga catttgccgc
    36421 accggattat gatcgttatc catgcctgaa actggcgatg gaggcgttcg aacaaggcca
    36481 ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt ttcttgcgca
    36541 acaaatccgc tttacggata tcgccgcgtt gaatttatcc gtactggaaa aaatggatat
    36601 gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcgc gtgaagtcgc
    36661 cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag agtcgcgcta
    36721 tttgttagcg tagggcttca gtgatatagt ctgcgccatc tgatcgtaag tagttggctt
    36781 tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct tcagtattcc
    36841 tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc tactcaacca
    36901 cttagcgaaa aattgccagc gcatggctgt cgtcatgttg cgatcattat ggacggcaat
    36961 ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc cggggcaaaa
    37021 tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaggcgtt aactctgtat
    37081 gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg
    37141 tttgtgtggg cgcttgatag cgaagtaaag agtctgcacc gacataacgt gcgtctgcgt
    37201 attattggcg ataccagtcg ctttaactcg cgtttgcaag aacgtattcg taaatctgaa
    37261 gcgctaacag ccgggaatac cggtctgacg ctgaatattg cggcgaacta cggtggacgt
    37321 tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa
    37381 ccagatcaga tagatgaaga gatgctaaac cagcatgtct gtatgcatga actggcccct
    37441 gtagatttag taattaggac tgggggggag catcgcatta gtaacttttt gctttggcaa
    37501 attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga tgaacaagac
    37561 tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg taccgagccc
    37621 ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat atctgctttt
    37681 gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg gttcgccatt
    37741 gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag cggttttacc
    37801 actcgctcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc gctgatgctt
    37861 tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat ctcactttgg
    37921 gcttcgctgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc aggttccgca
    37981 gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac cattgttccc
    38041 ttcttctggg gcatgctggc gttacgggcc tggcactacg acgagaatca ttacagtggc
    38101 gcaatatggc tgctctatgt catgatcctg gtatggggcg ctgactctgg cgcatatatg
    38161 tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg taaaacctgg
    38221 caaggcttta taggtggact cgcgactgca gcggtaatct catggggtta tggcatgtgg
    38281 gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc agcgttagcc
    38341 tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat taaggacagc
    38401 ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct gacggctgcg
    38461 gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg gaaggtaata
    38521 tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta cttatcaccg
    38581 tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct
    38641 caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc gaatatgtta
    38701 tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca gaaccggtcc
    38761 ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga gcggcgatta
    38821 ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg ctggttttta
    38881 ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat tcgatagctg
    38941 cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc gaaacgcctg
    39001 attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc accaccatta
    39061 cggtagcgcc atttggcagc gaccaacggc aagatgtaaa gctcgattta cgtcactggg
    39121 cgtttgagcc tgataaagaa gatccggtaa cttcgctggg gattcgtcct cgtgggccgc
    39181 aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag gcaggtttgc
    39241 aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg
    39301 tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa aggcagggga
    39361 gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa gcgattggtt
    39421 ttgtcggtat tgagccgaaa gtcattcctt tgcctgatga gtataaagtt gtacgccagt
    39481 atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagttg atgaagctga
    39541 cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc
    39601 cgatctctat cgccaagggg gctgggatga cagcggaact cggggttgtt tattacctgc
    39661 cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg ttgcccgtac
    39721 ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga ccggtatccg
    39781 agcgggttca agacttttgt tatcgcattg gctcgatttt gctggtgctg ttaatggggc
    39841 ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac gcataataac
    39901 gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg
    39961 tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg tcgccgttgg
    40021 tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg gttaatgatg aagatatcag
    40081 taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg tccttcgtga
    40141 tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca ttactttctc
    40201 cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac ctcgaggctt ctggcgtgcg
    40261 tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc tggaagactt
    40321 ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc cgctgccgcg
    40381 caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg tcagctgaaa tccagcaaat
    40441 taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt tccaactgcg
    40501 tgacgaagtg ccgtggtgga acgtggtcgg cgatcgtaaa taccagaaac agaaactggc
    40561 gggcgacctt gaaaccctgc gcagttacta tctggatcgc ggttatgccc gtttcaacat
    40621 cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca cggtgaacat
    40681 caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc ttgccgggca
    40741 ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata acggcaccaa
    40801 agtgaccaaa atggaagatg acattaaaaa gcttcttggt cgctatggtt atgcctatcc
    40861 gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat tacgcgtgaa
    40921 cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta acgatacctc
    40981 gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggc tggggagcga
    41041 tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac
    41101 cgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca aggtgaaaga
    41161 acgtaacacc ggtagcttca acttcgggat tggttacggt actgaaagtg gcgtgagctt
    41221 ccaggctggt gtgcaacagg ataactggtt aggtacgggt tatgctgttg gtatcaacgg
    41281 gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt acttcaccgt
    41341 agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag atgacgccga
    41401 cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct tcccgatcaa
    41461 cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt ccaacatgca
    41521 gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga gcacctctga
    41581 tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga cctacaacaa
    41641 gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac
    41701 cattccggga tcggataacg aatactacaa agtgacgtta gacacggcga cttatgtgcc
    41761 gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt atggtgatgg
    41821 tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt ccagcaccgt
    41881 gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc atcaggctag
    41941 taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga aagacctgtg
    42001 taaatcggat gatgctgtag gcggtaacgc tatggcggtt gccagcctcg agttcatcac
    42061 cccgacgccg ttcattagcg acaagtatgc taactcggtt cgtacttcct tcttctggga
    42121 tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat atccggacta
    42181 tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga tgtccccatt
    42241 ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag acaaggcaga
    42301 acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg
    42361 tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca aagaactgca
    42421 ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta ttagctgcag
    42481 gtctcggttt agcactggca acttctgctc aggcggctga caaaattgca atcgtcaata
    42541 tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg
    42601 agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag gctaaaatga
    42661 aaaagctgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa gacgtgatgg
    42721 ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt
    42781 ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa tccgttgcca
    42841 acagccagga tatcgatctg gttgttgatg caaacgccgt tgcttacaac agcagcgatg
    42901 taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct tcaattcgac
    42961 tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat atcgtcatca
    43021 ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat cacgttcatg gttaacccaa
    43081 aataccgtga gcatttaggc ttgtgccagg cgtccgcggt tgtcatgacc caggacgatc
    43141 ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact tacgcgcgca
    43201 tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga
    43261 tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgct gtgattgagt
    43321 ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca
    43381 gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat gagatccaga
    43441 tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg tgcagacggc tttggttatg
    43501 ccaacgatcg tggtaactgg gtgaagatcc cgcagatagg tcgcgtaatt attggcgatc
    43561 gcgtggagat cggcgcatgt acgaccatcg atcgcggcgc gctggatgac actgttattg
    43621 gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg attggcgaca
    43681 atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt cgttactgca
    43741 tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa gtgacggtta
    43801 cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc tcaggcattc
    43861 cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca
    43921 tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa cgttccatct
    43981 tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc cgtgttatta
    44041 ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc atactctgca
    44101 gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt
    44161 gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc
    44221 attcttccag ggccatttcc ctggaaaacc gattttcccg ggggtgctga ttctggaagc
    44281 aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga
    44341 gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga
    44401 tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg
    44461 ggttgctctg gtcgacggta aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg
    44521 ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga
    44581 gggcgcgtcg attggcgcga acgctcacat tggtcctttt tgtatcgttg gaccccatgt
    44641 cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc atactaaaat
    44701 tggccgcgat aatgagattt atcagttcgc ctccatcggc gaagttaacc aggatctgaa
    44761 atatgctggc gaaccgaccc gcgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt
    44821 caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca gcgacaactt
    44881 actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc
    44941 caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat
    45001 gaccgcagtc catcagttct gcatcattgg tgcgcacgtg atggttggcg gctgctccgg
    45061 tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa cgccgttcgg
    45121 tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta ccgctatccg
    45181 caatgcgtat aagctgattt atcgtagcgg taaaacgctc gatgaagtga aaccggaaat
    45241 tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct ttgcacgctc
    45301 aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat taacgattgc cctggtcgcc
    45361 ggagaaacct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg
    45421 cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg ctgcgaagcc
    45481 tggtacgaaa tggaagagct ggcggtgatg ggcattgttg aagtgctcgg gcgtctgcgt
    45541 cgcttactgc atattcgtgc cgatctgaca acgcgttttg gcgaactgaa gccagatgtt
    45601 tttgtcggta ttgatgcgcc tgacttcaat attacccttg aaggtaacct caaaaagcag
    45661 ggtatcaaaa ccattcatta cgtcagtccg tccgtctggg cgtggcgaca gaaacgcgtt
    45721 ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt
    45781 tatgacaaat acaacgtacc gtgccgcttt atcggtcata ccatggctga tgccatgccg
    45841 ttagatccag ataaaaatgg tgcccgtgat gtactgggga tccctcacga tgcccactgt
    45901 ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc cgatttcctg
    45961 aaaacggccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt gccgctggtg
    46021 aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagttgcgcc agacctgtcg
    46081 gttcatatgc tggatgggtt gggccgtgag gcgatggtcg ccagcgatgc ggcactactg
    46141 gcgtcgggga cggcagcact ggagtgcatg ctggcgaaat gcccgatggt agtgggatat
    46201 cgcatgaagc cttttacctt ctggctggcg aagcgactgg tgaaaactga ttacgtctct
    46261 ctacccaatt tgctggcggg cagagagtta gtcaaagagt tattgcagga agagtgtgag
    46321 ccgcaaaaac tggctgcggc gctgttaccg ctgctggcga acgggaaaac cagccacgcg
    46381 atgcacgaca ccttccgtga actgcatcag cagatccgct gcaatgccga tgagcaggcg
    46441 gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac acgcagctgg
    46501 ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg
    46561 tgatccttga cccggcgcgc ccaattgccg ggctgaatga ttccaaaaag ctgagcgaaa
    46621 aacgccgtct ggcgctctgt gaagagatca aagagaaagc gttgagctgg agtctgggcc
    46681 gcgcggaacc tcacgaaatc gacgaactga atattttaca tgcgaccatg ctggcgatgc
    46741 agcgtgccgt cgctggactg catattgcgc cggaatatgt gttgattgat ggtaaccgct
    46801 gcccgaaatt accgatgcct tcgatggctg tggtgaaagg cgatagccgc gtaccggaaa
    46861 tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata
    46921 ttgttttccc gcaatatggt tttgcccaac acaaagggta tccaaccgct tttcatctgg
    46981 aaaaactggc tgaacacggc gcgaccgaac accatcggcg cagctttggg cctgtcaaac
    47041 gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg aatctgaaga
    47101 tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg atcgatggcc
    47161 tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga
    47221 tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga catggcgcag
    47281 ggattaagcc catcgtcggg gcggatttta acgtccagtg cgacctgctg ggtgatgagt
    47341 taacccacct gacggtgctg gcggcgaaca ataccggcta tcagaatctg acgttgctga
    47401 tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat cgcgactggc
    47461 ttatcgaatt aaatgaaggg ttgatccttc tttccggcgg gcgcatgggc gacgttggac
    47521 gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt tatgaagaac
    47581 actttccgga tcgctatttt ctcgagctga tccgcaccgg caggccggac gaagaaagct
    47641 atctgcacgc tgcggtggaa ctggcggaat cacgcggttt gcccgttgtg gcgaccaacg
    47701 acgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc gcgatccacg
    47761 acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc
    47821 gtagcgaaga ggagatgtgc gagctgtttg ccgacatccc agaagccctt gccaacaccg
    47881 ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc ctgccgcagt
    47941 tcccgaccgg ggacatgagc accgaagatt atctggtcat gcgtgcaaaa gagggcctgg
    48001 aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaagcgt cgtccggagt
    48061 atgacgaacg tctggatact gaacttcagg ttatcaacca gatgggcttc ccgggttact
    48121 tcctcatcgt tatggaattt atccagtggt cgaaagataa cggtgtaccg gtagggccag
    48181 gccgtggttc tggtgcaggg tcactggtgg cctacgcgtt aaaaatcacc gatctcgatc
    48241 cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg
    48301 acttcgacgt tgacttctgt atggagaaac gtgaccaggt tatcgaacat gtggcggaca
    48361 tgtacggtcg cgatgcggta tcgcagatta tcaccttcgg tacgatggcg gcgaaagcgg
    48421 tgatccgcga cgtaggccgt gtactggggc atccgtacgg atttgtcgat cgtatctcga
    48481 aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc
    48541 tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg gcgcgcaaac
    48601 tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc gcaccgacca
    48661 aaattaccga ttttgcgccg ctttactgcg atgaagaggg caaacatccg gtcacccagt
    48721 ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt ggtttgcgta
    48781 cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg
    48841 agccgccgct ggatatcgcc gcgatcccgc tggatgataa gaaaagcttc gacatgctgc
    48901 aacgctcgga aaccactgcg gtattccagc ttgaatcgcg cggcatgaag gacctgatca
    48961 agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg ttccgccccg
    49021 gtccgttgca atcagggatg gtggataact ttatcgaccg taaacatggt cgcgaagaga
    49081 tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa accggtactg gagccaacct
    49141 acggcatcat cctgtatcag gaacaggtca tgcagattgc ccaggtgctt tctggttata
    49201 ccctcggtgg cgcggatatg ctgcgtcgtg cgatgggtaa gaaaaagccg gaagagatgg
    49261 ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac gccgaactgg
    49321 cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac aaatcgcact
    49381 ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac tatccggcgg
    49441 agtttatggc ggcggtaatg accgccgata tggacaatac cgagaaggtg gtgggcctgg
    49501 tggatgagtg ctggcggatg gggctgaaaa tcctgccgcc ggatataaac tccggtcttt
    49561 accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcg atcaaagggg
    49621 tcggtgaagg tccgattgag gcgatcatcg aagcccgtaa taaaggcggc tacttccgcg
    49681 aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg gtactggaaa
    49741 aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg ctgatgaact
    49801 cgctgggcga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct atcggtcagg
    49861 ccgatatgtt cggtgtgctg gccgaagagc cggaacaaat tgaacaatcc tacgccagct
    49921 gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta ggcctgtacc
    49981 tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc ggaggcgtaa
    50041 ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg gggctcgttg
    50101 ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc acgctggatg
    50161 accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa taccagcaat
    50221 tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat gacttcagcg
    50281 gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg gaaaaatatg
    50341 ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt ttaaaccgac
    50401 tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc tactatcaga
    50461 gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc
    50521 gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg gagtttgact
    50581 aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt gcagagctgg
    50641 aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg gatattaaca
    50701 tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt aaaatcttcg
    50761 ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccacagcgt ccttataccc
    50821 tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac cgcgcgtatg
    50881 cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg gtgatgatca
    50941 ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag
    51001 cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc tttaaaatgc
    51061 caatcatcac ctttatcgac accccggggg cttatccggg cgtgggcgca gaagagcgcg
    51121 gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttagc gtaccgacta
    51181 tttgtaccgt tatcggtgaa ggtggttctg gcggcgcgct ggcgattggc gtgggcgata
    51241 aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt tgtgcgtcca
    51301 ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt atcatcgctc
    51361 cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg ggcggcgctc
    51421 atcgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg gatctggccg
    51481 atctcgacgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct
    51541 acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc ccttttttat
    51601 cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac acatgaacat
    51661 cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca aagaactgga
    51721 gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg ttgatttgct
    51781 gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg atgagtacag
    51841 tctcgattta tgtagcgata tcaatcagct taatgaatat ctcccgcttt atgccttcat
    51901 caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc tctggttttt
    51961 tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt acaccgacga
    52021 atatcttgat aacattacac cgccgttcac gaaagccttg tttacctacg tcaaagagcg
    52081 gaagtacacc ttttgtacgc cgggtcatat gggcggcacc gcatatcaaa aaagcccggt
    52141 tggctgtctg ttttatgatt ttttcggcgg gaatactctt aaggctgatg tctctatttc
    52201 ggttaccgag cttggttcgt tgctcgacca caccgggcca cacctggaag cagaagagta
    52261 catcgcgcgg acttttgacg cggaacagag ttatatcgtt accaacggaa catcgacgtc
    52321 taacaaaatt gtgggtatgt acgccgcgcc gtccggcagt acgctgttaa ttgaccgcaa
    52381 ttgtcataaa tcgctggcgc atctgttgat gatgaacgat gtagtgccag tctggctgaa
    52441 accgacgcgt aatgcgttgg ggattcttgg tgggatcccg cgccgtgaat tttctcgcga
    52501 cagtatcgaa gagaaagtcg ctgccaccac gcaagcacaa tggccggttc atgcggtgat
    52561 caccaactca acctatgatg gcttgctcta caacaccgac tggatcaaac agacgctgga
    52621 tgtcccctcg atccacttcg attccgcctg ggtgccgtac acccattttc atccgatcta
    52681 ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagtgatct tcgaaacgca
    52741 atcgacccac aaaatgctgg cggcgttatc gcaggcttcg ctgatccaca ttaaaggcga
    52801 gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct cgcccagtta
    52861 tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc cgggcaaacg
    52921 gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc agcggctgcg
    52981 ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga
    53041 atgctggccc gttgcgcctg gcgaacagtg gcacggcttt aacgatgcgg atgccgatca
    53101 tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg agcagggcaa
    53161 tatgagcgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg aacgagggat
    53221 cgtagtagag aaaaccggtc cttataacct gctgtttctc tttagtattg gaatcgataa
    53281 aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt acgatctcaa
    53341 cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt tctaccgcaa
    53401 tatgcgcatt caggatctgg cgcaagggat ccataagctg attcgtaaac acgatcttcc
    53461 cggtttgatg ttgcgggcat ttgatacctt gccggagatg atcatgacgc cacatcaggc
    53521 atggcagaga cagattaaag gcgaagtgga aaccattgcg ctggaacaac tggtcggtag
    53581 agtatcggca aatatgatcc tgccttatcc acctggcgta ccgctgctga tgccgggaga
    53641 aatgctgacc aaagagagcc gcacagtact cgattttcta ctgatgcttt gttccgtagg
    53701 gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg aagacggcgt
    53761 ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt ccgggcgagt
    53821 aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt tcaccatatt
    53881 gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat tcttggtttc
    53941 acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt
    54001 aatgggcaat atgtgattga gcttttctca tttccgttcc cgccggagcg ccccagccga
    54061 ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgatatcga tgcggcagtg
    54121 gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa
    54181 aaacgcttca cgttctttaa cgatccggac gggctgccgt tggaactgta tgagcagtaa
    54241 ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt aaaatcgcac
    54301 catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc tggtggcctt
    54361 tagcggcggg cttgactcca ccgttttgct gcatcagttg gtgcagtggc gggcggaaaa
    54421 tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca atgccgacgc
    54481 ctgggttagg cattgcgaga acatctgcca acaatggcag gtgccgctgg tggtcgagcg
    54541 cgtgcaactt gcgcaagagg ggctaggtat tgaggcccag gcgcggcagg cacgttatca
    54601 ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc accgcgcaac atctcgacga
    54661 tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg ggctttcggc
    54721 tatgggggaa gtttcggagt ttgccggaac acggcttatc cgcccgttgc tcgcccgcac
    54781 gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg aagacgaaag
    54841 taatcaggat gatagctacg atcgtaactt cctgcgcctg cgcgtggtgc cgttattgca
    54901 gcagcgttgg ccgcattttg ccgaagcaac ggcccgcagc gctgcgcttt gcgctgaaca
    54961 agagagcctg ttagatgaac tgctggcgga tgatatagcg cactgtcaaa cgtcgcaggg
    55021 gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgccgcgcgg cgattatccg
    55081 ccgctggctg gcagggaaga atgcaccgat gccttcccgc gacgcgttgg tgaggatctg
    55141 gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgcctgg gcgcgtttga
    55201 aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcactgggc aaagcgaaac
    55261 cattgtgccg tggcagacgt ggctacaacc gctggaatta ccggcgggac tgggaagtgt
    55321 acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg tcagcgtacg
    55381 tttcaaagcg ccaggattgc tgcatattat cgggcgcaac ggcggacgta agctgaagaa
    55441 aatctggcag gagctgggcg ttccgccgtg gctacgtgac accacgccac tgctgtttta
    55501 tggcgaaacg ctgattgcgg cggcaggagt atttatgacg caagaaggta tggctgaaag
    55561 tgagaatgga gtaagttttg tctggcagag aaatggttag tcagtgaagg ccggatacgc
    55621 atcaaacgtc gcatccggca aaagtcaatc aggactcgct caccaccacc gtaccgattt
    55681 ccgggtggct aaagctggta attttatcca gacgcagctc gcgggttgta ccagcggcct
    55741 cgacgaccag atactccaca tttttgcggg agactaaatc gctggccttc gcctgcaatt
    55801 tttcgccatc tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat
    55861 tatcgtaatc atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc
    55921 agtaagtttg ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg
    55981 gcagccattt cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag
    56041 tctccgctgg cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc
    56101 cctcctgtca gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc
    56161 tgtttcgcaa ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt
    56221 attccgttac aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa
    56281 tttataaagc gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc
    56341 tgacgctggc gcgccatcct tcagaaaccc aggagcgtat gatgctgcgc ctgctggcgt
    56401 ggctaaaata tgccgatgaa cgtctgcaat ttacccgtgg cttgtgtgcc gatgatgagc
    56461 cggaagcgtg gcggcgtaac gatcatttgg gcattgattt gtggattgag ctggggctgc
    56521 cggatgagcg gcggattaag aaagcctgca cccaggccgc agaagtagcg ctgtttgcct
    56581 ataatagtcg ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gcgcagtttg
    56641 ccaatctttc cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg
    56701 atcgtaccat gacgctgcag gcaacgattc aggatggcgt gatctggtta tcggatgata
    56761 agaataatct ggaagtgaac ttaaccgtct ggcaacaacc ttcatgattg tgatttcccg
    56821 acatgttgct atccccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg
    56881 cgggcagcat gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc
    56941 cagcctgcca gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag
    57001 tgatggcgtg attgtcatta aggcacagga ataccgcaat caggagctca accgcgaagc
    57061 ggcgctggcc cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgacg
    57121 acccacgcgg cccacccgcg catcaaaaga gcgcaggctg gcatcgaaag cacaaaaatc
    57181 aagcgtgaag gcgatgcgcg gcaaagtgcg cagcggtcgg gaataaaaag aaggaatgga
    57241 tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat
    57301 gtaataatcg ggccgaagtc gacacgcttt ctccggcgca ggctgccgaa ctgaaaccga
    57361 tgccgcaaag ttggcgcggc gtgctgccgt gtgccgattg cgaagggatc gaaacctctc
    57421 tgttcctcga aaaagatgga acctgggtga tgaatgaacg ctatcttggt gcgcgtgaag
    57481 agccttcctc tttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa
    57541 ccgacagcaa aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg
    57601 atcgtgaagg taatccgatt gaatcgcagt tctactatac gctggaaccg gcgcaatcca
    57661 gtttacccat gacgccgatg accctgcggg gcatgtattt ttatatggct gatgcggcga
    57721 ccttcactga ttgcgcgacc ggaaaacgtt tcatggtagc gaataatgca gagctggagc
    57781 gtggctacct ggcggcgcgc ggtcatagtg aaaaaccgat gttactgtca gtagaaggtc
    57841 actttacgct tgaggctaat ccggataccg gtgcgccaac caaagtatta gcgcccgata
    57901 cggcaggtaa attttacccg aaccaggatt gcagtagttt ggggcagtaa ttgaaagccg
    57961 gagcattctc cggctttcat cactgagtca gcccaacaaa gcgacccgcc ttgatataaa
    58021 tccctggctt caggtgccca acatactgta actgcgtttc tggcttaaac gcagagacat
    58081 caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc gccagggtaa
    58141 gcggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac cacgactttt
    58201 tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg cactgttgac
    58261 gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata aagggaataa
    58321 attcgacaat gccgcgatag ttgtaaccgc tatctttaat tttgttggcg aacgcggtga
    58381 tttctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc ttatcgctat
    58441 gcttcatcgc ttttttgagg gaaacaatct ggacgccagc gcctgtcgct tctgcaacgt
    58501 tattttcacc cagatagatt gcaacgtgac tcacagagga agtgctgaag acgcggattc
    58561 caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg agatcaggtt
    58621 ccgttatttc ttttattgat tgttcggtaa aagagctttg atgctggaat tggactgccc
    58681 atgttttcgc cccggcatct accgctgtgg ctgatgagtc tggctggctg atatctacgg
    58741 tacaggccga aaggaaaaga aagcagggga gaagcaggca gcaatgcgcc tttggtttat
    58801 ccattttata caagccatgt aaaaaagggc cctgaaattc aggacccttt ctggcatcag
    58861 cctttaatct gtttcaccag ataatcgacg atgtcaccag tcttaattaa ctgtttctcg
    58921 ccgttacgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc gcccagcaca
    58981 atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg
    59041 cggtcatcca gcagcacttc gataccctgc gcgcgcagtt cgctgtacag tttctcagca
    59101 agctcctgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac ctggaacggt
    59161 gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat cgccgcagct
    59221 actacacgcg ttactccgat accgtagcaa cccatcgtca ggatttgatt acggccatct
    59281 tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg gaagatgtga
    59341 ccaacttcga taccacgttt gatcagcagt gtaccctggc catccgggct tggatcgccc
    59401 gccaccacgt tacggatatc agcaatttct ggggtagcga catcgcgatc ccagttgatg
    59461 ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat cgctgcaacg
    59521 gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct
    59581 ttaaccacgg cacgaatttc ttcttcggtc gcaaaagtca gcgggcttgc aacctgcggc
    59641 agtttttctg ctttaacttc gttcagctcg tggtcaccac gcaccagcag cgcaaccagc
    59701 gggaagctac tgccttcaac cgctttaacc agcagagtct tcaccgtttt ctcaatcggc
    59761 agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc aaccagcgtc
    59821 atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagctcaatg
    59881 ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct ctgcgccagc
    59941 acctggaatt cgtgagaggc gctaccgccg atagaaccgg tgtcggcctg tacggcgcgg
    60001 aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt
    60061 tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg
    60121 gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag
    60181 aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc tgtaataact
    60241 tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa acgcagcagt
    60301 tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg ctgaaccacc
    60361 ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact
    60421 tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg
    60481 atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc
    60541 ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt
    60601 agaacaggct caaggtgagc ctgggacaaa aaaagtgatt tagtttacca gtgcaaaaga
    60661 aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact tcaaaacctg
    60721 cgtcggtgac gcgccagcga acgttaaaat catgcagcca gacggcatag gttttgcccg
    60781 tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg cggataaaca
    60841 gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc tctgcggtaa
    60901 aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg gcatcgggaa
    60961 gcgattcggc aaaggggaga tacggtttga tatccaccac cggcgtccca tctaccagat
    61021 ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct ttcagctcta
    61081 ccagcgacat accaatcggg ttagggcgga aagtggagcg cgtagcgaaa acacccattc
    61141 tggtattacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct tccatcgttt
    61201 gatgaaagac gaaaaggatc cataaatggc tgaacgcttc caggccgcga acggcgtcgg
    61261 cctggttgta gggagcaatg agatgcagtt cgccgttggc gctttttacc agacctggct
    61321 ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt tgctcgaatt
    61381 ggaaactgct catttcgccg tgatgttaag cgcagaaccg atacatacag cctgacgata
    61441 gcaacctggc gtaccgctgg tgacttcgca gctatgcagt aataccgcat tggctttcat
    61501 tttagaggcg ttgatttgca tccgcttacg tgcggttgga atgctcggcg gagagtcctg
    61561 attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg gtttgccgac
    61621 taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt tcggttttgc
    61681 aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg atctgcttaa
    61741 catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac gcatagtatt
    61801 tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa tgacaagacg
    61861 ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa attaccagcc
    61921 tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcgt cagactgata
    61981 agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg tcacgatcag
    62041 gtttacgtac ggggagtctt tatcttcgac aaagataccg tctttcgccg gagtcaggcc
    62101 aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt cgtccagaga
    62161 gcgaggcagc tgcggtgctt ccagctcaac aattttcaga ttttttgggt tctcaacaac
    62221 atcaagaacg gtcggcagca ggccaacgcc atctttcagt ttgatcaagc ccactttttg
    62281 cagcagcagc agtgaacgac caaggttagt tgggtcgttt ggcacggcaa cctgcgaacc
    62341 atcctgcagt tcatccagtg atttgatttt cttggagtaa cctgcaatcg gataaacaaa
    62401 agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat caaggtacgg
    62461 tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt ttggcagaac
    62521 atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg caactttctg
    62581 cgcgacttct gcaacctgct gttcggcacc aacaatcacg ccgactttaa tgtggtttgg
    62641 atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg ctcccactgc
    62701 cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt tatgttgtgt
    62761 tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact gaattaaata
    62821 aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt agccgatgta
    62881 gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac ccatcgcgga
    62941 ataaccgacc agggtaatca gggtgatagt cgcagcattc accagacccg gcaatgcttc
    63001 cggtaacagc actttacgga cgatctgtat cggcgtggca cccattgcgc gggaagcttc
    63061 aattagcccg gttgggatct ccagcagagc gttctcgacc atacgggcaa taaacggtgc
    63121 tgcaccaacg gttaacggaa caatcgctgc ctgcaaacca atcgatgtac cgacaataac
    63181 gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatagaac ggaaaatgtt
    63241 cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc ccgggcgcgt
    63301 aacataaagc aggacgccga ccggcagacc aatcacaaac ccaaaaaatc cggatacgaa
    63361 ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg gctcagacat
    63421 aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg gcttgcgtat
    63481 cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa cttaacgcca ccggcgtaat
    63541 ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt tcagaaagca
    63601 gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg cagtcagtga
    63661 atggctccgc ttgcagacgc tcctgataat cttccgggat atccagatgc agggtcgact
    63721 gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt accgtgtcct
    63781 gctcgatcag ttcgccatta ctgatgaccg ccacgcaatc acaaatgcgc ttcacaacgt
    63841 ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg tctttcagca
    63901 gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca tcacacagca
    63961 atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc tgcccaccgg
    64021 aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac agcaattccg
    64081 tcacgcgacg tttgatctcg tctttcggtg tattgtccag ctccagcggc agagccacgt
    64141 tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata ccaatctggc
    64201 ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag ttcctgtcca tcgaccagca
    64261 cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta ctcttacccg
    64321 cgcctgaggc accgataacg ccataaattt gcccagctgg cacatgcagg ctgacgttgt
    64381 tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc gaaagtttta
    64441 tcattgatta tttattatcg tcattaagtt agtcgtggca tttcgaatgc ctgaaacggg
    64501 caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat caggtttatg
    64561 cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcacatgc cttattaagg
    64621 agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat ggcaccatta
    64681 atgtcgatca cggctatgtc catgagatcg ataactttga atttatcgac ggtgttattg
    64741 acgccatgcg cgagctaaaa aaaatgggct tcgcgctggt ggtagtcacc aaccagtctg
    64801 gcattgctcg cggtaaattt actgaagcac agtttgaaac gctgaccgag tggatggact
    64861 ggtcgctggc ggaccgcgat gtcgatctgg atggtatcta ttattgcccg catcatccgc
    64921 agggtagtgt tgaagagttt cgccaggtct gcgactgccg caaaccacat ccggggatgc
    64981 ttttgtcggc acgcgattat ttgcatattg atatggctgc ttcttatatg gtgggcgata
    65041 aattagaaga tatgcaggca gctgctgcgg cgaacgtggg aacaaaagtg ctggtgcgta
    65101 cgggtaaacc tgttacgcct gaagcagaaa acgcggcgga ttgggtgtta aatagcctgg
    65161 cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gtaatgatta aaagatgagc
    65221 ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct ataatgcgcc
    65281 tccatcgaca cggcggatgt gaatcacttc acacaaacag tcggttcggt tgaagagaaa
    65341 aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc acctcgcgac
    65401 agtgagctga aagccgcgtc
//
LOCUS       DAYPHN010000008        64725 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000008 DAYPHN010000000
VERSION     DAYPHN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.008"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..352
                     /locus_tag="ACG0BW_000513"
     CDS             <1..352
                     /locus_tag="ACG0BW_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFK7257527.1"
                     /translation="AMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQN
                     MDQFWSPSEQYGVQQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLH
                     SFELAMDVKEELLG"
     gene            complement(330..1049)
                     /locus_tag="ACG0BW_000514"
     CDS             complement(330..1049)
                     /locus_tag="ACG0BW_000514"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HFK7257528.1"
                     /translation="MGRKGLLAIVLLSLFIAMIFQFFWPAPHDEHVFLPVEKPVAPSL
                     KIIHPGDQLFIRILKAEDKLELWASTNNKPYELYKTRTICAWSGGLGPKHKQGDGKSP
                     EGFYATNKELLNPNSRYHLAFNIGYPNAYDRANGYTGDFIMVHGNCVSAGCYAMTDAG
                     IEEIYQLVAQALNSGQKSVPVHIFPFAMDEENMRQAQVWPEYNFWRMLKPGYDYFEKN
                     RRLPTITVENRRYKISPTTLP"
     gene            1207..1737
                     /locus_tag="ACG0BW_000515"
     CDS             1207..1737
                     /locus_tag="ACG0BW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFK7257529.1"
                     /translation="MSEPASFFLHAHITESNLEKFFHSPATNIKDHDDWLPWFIEKQR
                     LYGDPAKMLNNLAACNSGESEKNIYAEHINFNKETQIVTMDHIFLSESYEIFMPLMAC
                     VRGIEKFITPGENNFALIYYYWWGSETAIALEFDANGSKITANPKAENLTIADAFFDE
                     HGEALAEELYNKQGFI"
     gene            complement(1798..3042)
                     /gene="mtr"
                     /locus_tag="ACG0BW_000516"
     CDS             complement(1798..3042)
                     /gene="mtr"
                     /locus_tag="ACG0BW_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFK7257530.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MTALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(3196..5097)
                     /gene="deaD"
                     /locus_tag="ACG0BW_000517"
     CDS             complement(3196..5097)
                     /gene="deaD"
                     /locus_tag="ACG0BW_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFK7257531.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDST
                     GRRRFGGDA"
     gene            complement(5090..5170)
                     /gene="yrbN"
                     /locus_tag="ACG0BW_000518"
     CDS             complement(5090..5170)
                     /gene="yrbN"
                     /locus_tag="ACG0BW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFK7257532.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(5277..6161)
                     /gene="nlpI"
                     /locus_tag="ACG0BW_000519"
     CDS             complement(5277..6161)
                     /gene="nlpI"
                     /locus_tag="ACG0BW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFK7257533.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLRQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(6270..8405)
                     /gene="pnp"
                     /locus_tag="ACG0BW_000520"
     CDS             complement(6270..8405)
                     /gene="pnp"
                     /locus_tag="ACG0BW_000520"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFK7257534.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(8652..8921)
                     /gene="rpsO"
                     /locus_tag="ACG0BW_000521"
     CDS             complement(8652..8921)
                     /gene="rpsO"
                     /locus_tag="ACG0BW_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFK7257535.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(9070..10014)
                     /gene="truB"
                     /locus_tag="ACG0BW_000522"
     CDS             complement(9070..10014)
                     /gene="truB"
                     /locus_tag="ACG0BW_000522"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFK7257536.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(10014..10415)
                     /gene="rbfA"
                     /locus_tag="ACG0BW_000523"
     CDS             complement(10014..10415)
                     /gene="rbfA"
                     /locus_tag="ACG0BW_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFK7257537.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(10574..13246)
                     /gene="infB"
                     /locus_tag="ACG0BW_000524"
     CDS             complement(10574..13246)
                     /gene="infB"
                     /locus_tag="ACG0BW_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFK7257538.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(13271..14758)
                     /gene="nusA"
                     /locus_tag="ACG0BW_000525"
     CDS             complement(13271..14758)
                     /gene="nusA"
                     /locus_tag="ACG0BW_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFK7257539.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(14786..15238)
                     /gene="rimP"
                     /locus_tag="ACG0BW_000526"
     CDS             complement(14786..15238)
                     /gene="rimP"
                     /locus_tag="ACG0BW_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFK7257540.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(15445..15521)
                     /locus_tag="ACG0BW_000527"
     tRNA            complement(15445..15521)
                     /locus_tag="ACG0BW_000527"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15485..15487),aa:Met,seq:cat)
     gene            15869..17212
                     /gene="argG"
                     /locus_tag="ACG0BW_000528"
     CDS             15869..17212
                     /gene="argG"
                     /locus_tag="ACG0BW_000528"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFK7257541.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(17220..18845)
                     /gene="yhbX"
                     /locus_tag="ACG0BW_000529"
     CDS             complement(17220..18845)
                     /gene="yhbX"
                     /locus_tag="ACG0BW_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFK7257542.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVILFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDFVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(19304..19390)
                     /locus_tag="ACG0BW_000530"
     tRNA            complement(19304..19390)
                     /locus_tag="ACG0BW_000530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19354..19356),aa:Leu,seq:gag)
     gene            complement(19405..19737)
                     /gene="secG"
                     /locus_tag="ACG0BW_000531"
     CDS             complement(19405..19737)
                     /gene="secG"
                     /locus_tag="ACG0BW_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFK7257543.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(19965..21302)
                     /gene="glmM"
                     /locus_tag="ACG0BW_000532"
     CDS             complement(19965..21302)
                     /gene="glmM"
                     /locus_tag="ACG0BW_000532"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFK7257544.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(21295..22143)
                     /gene="folP"
                     /locus_tag="ACG0BW_000533"
     CDS             complement(21295..22143)
                     /gene="folP"
                     /locus_tag="ACG0BW_000533"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFK7257545.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(22233..24167)
                     /gene="ftsH"
                     /locus_tag="ACG0BW_000534"
     CDS             complement(22233..24167)
                     /gene="ftsH"
                     /locus_tag="ACG0BW_000534"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFK7257546.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(24267..24896)
                     /gene="rlmE"
                     /locus_tag="ACG0BW_000535"
     CDS             complement(24267..24896)
                     /gene="rlmE"
                     /locus_tag="ACG0BW_000535"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFK7257547.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            25022..25315
                     /gene="yhbY"
                     /locus_tag="ACG0BW_000536"
     CDS             25022..25315
                     /gene="yhbY"
                     /locus_tag="ACG0BW_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFK7257548.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(25471..25947)
                     /gene="greA"
                     /locus_tag="ACG0BW_000537"
     CDS             complement(25471..25947)
                     /gene="greA"
                     /locus_tag="ACG0BW_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFK7257549.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            26195..27628
                     /gene="dacB"
                     /locus_tag="ACG0BW_000538"
     CDS             26195..27628
                     /gene="dacB"
                     /locus_tag="ACG0BW_000538"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFK7257550.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNKIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(27668..28840)
                     /gene="cgtA"
                     /locus_tag="ACG0BW_000539"
     CDS             complement(27668..28840)
                     /gene="cgtA"
                     /locus_tag="ACG0BW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFK7257551.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(28856..29821)
                     /gene="yhbE"
                     /locus_tag="ACG0BW_000540"
     CDS             complement(28856..29821)
                     /gene="yhbE"
                     /locus_tag="ACG0BW_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFK7257552.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(29948..30205)
                     /gene="rpmA"
                     /locus_tag="ACG0BW_000541"
     CDS             complement(29948..30205)
                     /gene="rpmA"
                     /locus_tag="ACG0BW_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFK7257553.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(30226..30537)
                     /gene="rplU"
                     /locus_tag="ACG0BW_000542"
     CDS             complement(30226..30537)
                     /gene="rplU"
                     /locus_tag="ACG0BW_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFK7257554.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            30796..31767
                     /gene="ispB"
                     /locus_tag="ACG0BW_000543"
     CDS             30796..31767
                     /gene="ispB"
                     /locus_tag="ACG0BW_000543"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFK7257555.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            31994..32272
                     /gene="sfsB"
                     /locus_tag="ACG0BW_000544"
     CDS             31994..32272
                     /gene="sfsB"
                     /locus_tag="ACG0BW_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFK7257556.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPLTHEFIDRTQLIRSYTKPKK"
     gene            complement(32320..33579)
                     /gene="murA"
                     /locus_tag="ACG0BW_000545"
     CDS             complement(32320..33579)
                     /gene="murA"
                     /locus_tag="ACG0BW_000545"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFK7257557.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(33634..33888)
                     /gene="ibaG"
                     /locus_tag="ACG0BW_000546"
     CDS             complement(33634..33888)
                     /gene="ibaG"
                     /locus_tag="ACG0BW_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFK7257558.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(34047..34340)
                     /gene="mlaB"
                     /locus_tag="ACG0BW_000547"
     CDS             complement(34047..34340)
                     /gene="mlaB"
                     /locus_tag="ACG0BW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFK7257559.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(34340..34975)
                     /gene="mlaC"
                     /locus_tag="ACG0BW_000548"
     CDS             complement(34340..34975)
                     /gene="mlaC"
                     /locus_tag="ACG0BW_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFK7257560.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(34994..35545)
                     /gene="mlaD"
                     /locus_tag="ACG0BW_000549"
     CDS             complement(34994..35545)
                     /gene="mlaD"
                     /locus_tag="ACG0BW_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFK7257561.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(35550..36332)
                     /gene="mlaE"
                     /locus_tag="ACG0BW_000550"
     CDS             complement(35550..36332)
                     /gene="mlaE"
                     /locus_tag="ACG0BW_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFK7257562.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(36340..37149)
                     /gene="mlaF"
                     /locus_tag="ACG0BW_000551"
     CDS             complement(36340..37149)
                     /gene="mlaF"
                     /locus_tag="ACG0BW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFK7257563.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            37359..38336
                     /gene="yrbG"
                     /locus_tag="ACG0BW_000552"
     CDS             37359..38336
                     /gene="yrbG"
                     /locus_tag="ACG0BW_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFK7257564.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQSGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            38350..39336
                     /gene="kdsD"
                     /locus_tag="ACG0BW_000553"
     CDS             38350..39336
                     /gene="kdsD"
                     /locus_tag="ACG0BW_000553"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFK7257565.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSISDV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            39357..39923
                     /gene="kdsC"
                     /locus_tag="ACG0BW_000554"
     CDS             39357..39923
                     /gene="kdsC"
                     /locus_tag="ACG0BW_000554"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFK7257566.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLLPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            39920..40495
                     /gene="lptC"
                     /locus_tag="ACG0BW_000555"
     CDS             39920..40495
                     /gene="lptC"
                     /locus_tag="ACG0BW_000555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFK7257567.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            40464..41021
                     /gene="lptA"
                     /locus_tag="ACG0BW_000556"
     CDS             40464..41021
                     /gene="lptA"
                     /locus_tag="ACG0BW_000556"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFK7257568.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            41028..41753
                     /gene="lptB"
                     /locus_tag="ACG0BW_000557"
     CDS             41028..41753
                     /gene="lptB"
                     /locus_tag="ACG0BW_000557"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFK7257569.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            41801..43234
                     /gene="rpoN"
                     /locus_tag="ACG0BW_000558"
     CDS             41801..43234
                     /gene="rpoN"
                     /locus_tag="ACG0BW_000558"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFK7257570.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            43257..43544
                     /gene="hpf"
                     /locus_tag="ACG0BW_000559"
     CDS             43257..43544
                     /gene="hpf"
                     /locus_tag="ACG0BW_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFK7257571.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            43662..44153
                     /gene="ptsN"
                     /locus_tag="ACG0BW_000560"
     CDS             43662..44153
                     /gene="ptsN"
                     /locus_tag="ACG0BW_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFK7257572.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            44199..45053
                     /gene="rapZ"
                     /locus_tag="ACG0BW_000561"
     CDS             44199..45053
                     /gene="rapZ"
                     /locus_tag="ACG0BW_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFK7257573.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            45050..45322
                     /gene="npr"
                     /locus_tag="ACG0BW_000562"
     CDS             45050..45322
                     /gene="npr"
                     /locus_tag="ACG0BW_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFK7257574.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            45535..46167
                     /gene="yrbL"
                     /locus_tag="ACG0BW_000563"
     CDS             45535..46167
                     /gene="yrbL"
                     /locus_tag="ACG0BW_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFK7257575.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQILKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(46164..46892)
                     /gene="mtgA"
                     /locus_tag="ACG0BW_000564"
     CDS             complement(46164..46892)
                     /gene="mtgA"
                     /locus_tag="ACG0BW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFK7257576.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MAERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRG
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(46889..47542)
                     /gene="elbB"
                     /locus_tag="ACG0BW_000565"
     CDS             complement(46889..47542)
                     /gene="elbB"
                     /locus_tag="ACG0BW_000565"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFK7257577.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(47772..50108)
                     /gene="arcB"
                     /locus_tag="ACG0BW_000566"
     CDS             complement(47772..50108)
                     /gene="arcB"
                     /locus_tag="ACG0BW_000566"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFK7257578.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(50204..51133)
                     /gene="yhcC"
                     /locus_tag="ACG0BW_000567"
     CDS             complement(50204..51133)
                     /gene="yhcC"
                     /locus_tag="ACG0BW_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFK7257579.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            51808..56268
                     /gene="gltB"
                     /locus_tag="ACG0BW_000568"
     CDS             51808..56268
                     /gene="gltB"
                     /locus_tag="ACG0BW_000568"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFK7257580.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     YTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            56281..57699
                     /gene="gltD"
                     /locus_tag="ACG0BW_000569"
     CDS             56281..57699
                     /gene="gltD"
                     /locus_tag="ACG0BW_000569"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFK7257581.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            57883..59010
                     /gene="yhcG"
                     /locus_tag="ACG0BW_000570"
     CDS             57883..59010
                     /gene="yhcG"
                     /locus_tag="ACG0BW_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFK7257582.1"
                     /translation="MESLSERTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGMPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLILCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(59070..59534)
                     /gene="nanQ"
                     /locus_tag="ACG0BW_000571"
     CDS             complement(59070..59534)
                     /gene="nanQ"
                     /locus_tag="ACG0BW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFK7257583.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEVFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPSEIKKVVVKVKADLMA"
     gene            complement(59531..60406)
                     /gene="nanK"
                     /locus_tag="ACG0BW_000572"
     CDS             complement(59531..60406)
                     /gene="nanK"
                     /locus_tag="ACG0BW_000572"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFK7257584.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLMALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQWLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(60403..61092)
                     /gene="nanE"
                     /locus_tag="ACG0BW_000573"
     CDS             complement(60403..61092)
                     /gene="nanE"
                     /locus_tag="ACG0BW_000573"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFK7257585.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(61140..62630)
                     /gene="nanT"
                     /locus_tag="ACG0BW_000574"
     CDS             complement(61140..62630)
                     /gene="nanT"
                     /locus_tag="ACG0BW_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFK7257586.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(62739..63632)
                     /gene="nanA"
                     /locus_tag="ACG0BW_000575"
     CDS             complement(62739..63632)
                     /gene="nanA"
                     /locus_tag="ACG0BW_000575"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFK7257587.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(63755..64546)
                     /gene="nanR"
                     /locus_tag="ACG0BW_000576"
     CDS             complement(63755..64546)
                     /gene="nanR"
                     /locus_tag="ACG0BW_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFK7257588.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQSTNKKK"
BASE COUNT        15898 a        17514 c        15947 g        15366 t
ORIGIN      
        1 tgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt
       61 tacctcgatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc
      121 gccgattcaa aatatggatc agttctggtc accgtccgag cagtatggcg tgcagcaggc
      181 gctgagtatg tcgctggtgg gcgataaagc gaaagtgcgt catggtttgc agtcgatcct
      241 gcgcgaaacc gacgccgatg agattatggt taacgggcag attttcgatc accaggcgcg
      301 cctgcattcg tttgaactgg cgatggatgt taaggaagag ttgttgggct gattttataa
      361 cgccggtttt caacggtgat ggttggcaat cggcgatttt tttcaaagta atcatagccg
      421 ggcttcaaca tacgccagaa attatattcc ggccatacct gcgcctggcg catattttct
      481 tcgtccatcg caaacgggaa gatatgcacc ggcacgctct tctgcccgct attcagcgcc
      541 tgcgccacca gttgataaat ctcttcaata ccagcatcgg tcatggcata gcagcccgcc
      601 gaaacgcagt ttccgtgaac cataatgaaa tcaccggtgt agccgtttgc ccgatcgtaa
      661 gcattcgggt aaccaatatt aaatgccaga tgatagcggc tgtttgggtt aagcaactct
      721 ttgttggtgg cataaaaacc ttccgggctt ttaccatccc cctgtttgtg ttttggccct
      781 aagccaccag accacgcgca tatagtcctg gttttataga gttcgtaagg cttgttgttg
      841 gtgcttgccc acagttccag cttatcttcc gctttcagaa tgcggataaa taactggtca
      901 ccaggatgaa tgatttttaa agacggtgcg acaggttttt caactggcag gaaaacgtgt
      961 tcatcgtgtg gggcaggcca gaaaaattga aaaatcatgg cgataaacag gctcaacaat
     1021 acaatagcaa gtagtccttt acgccccatt tcgtattccg cttattctga gccagttcaa
     1081 gtatatattt tcttatgata tgaaatagat gaaaggtcca gttctgtact tttataaaat
     1141 agtcatcgtt ttatcatcgc caaacagaga aatgaacaaa tcgacttatt accagagaaa
     1201 atcattatga gtgaaccggc ttcctttttt cttcatgcac atattaccga gagtaacctc
     1261 gagaagtttt tccattcacc agcaacaaat atcaaagacc atgacgactg gttgccatgg
     1321 tttatcgaga agcagcggtt gtacggcgat cctgcaaaaa tgcttaacaa tcttgcagct
     1381 tgtaattctg gtgaaagtga aaagaatatt tatgccgaac atatcaactt taataaagaa
     1441 acacagatag tcacgatgga tcacatattt ctctcagaga gctatgaaat cttcatgcca
     1501 ctcatggcct gtgtgcgggg aatcgagaag tttattacgc caggggaaaa taattttgcg
     1561 ctaatttatt attactggtg gggttcggag accgctatcg cgctcgaatt tgatgccaat
     1621 ggtagcaaaa taacggcaaa tcctaaggca gaaaatttaa ctatcgccga tgcttttttc
     1681 gatgagcacg gggaagctct cgctgaagaa ctttataata aacaggggtt tatttgatac
     1741 catctcgtgt acttgtgaat tattgccgga tgaaaactgc tcatccggct cgttcaatta
     1801 ctgatacacc ggcagcaaat taaagctcga taaaatatgc accagtgcgt taccgacgcc
     1861 aaacaccaga atcagcgcaa tcatcggctt gccgccccag acgcggaatt tcgggctacc
     1921 aaagcgttta cgcgatgcgc gggccagcag cgccggaacg attgccgccc agatggtagc
     1981 cgctaagcca gcataaccaa tggcgtacag gaatccgttc gggaacagca aaccacccac
     2041 aacaggcggt gcaaaggtca acaatgctgt tttcaagcgg cccatagccg agtcatcgaa
     2101 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaacttgc
     2161 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
     2221 gcttaatgcc tgtaccagca catcaatgtt accgcccttc tcggcgatgc cgataaactc
     2281 cggacgaggg atgttgccca tcgtcgccag caaccagatg gtatacagcg ccagcgccat
     2341 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
     2401 caggctcggc acgttaccgt gataaccaaa cgatgccaga cagaatggca gtgtcatcag
     2461 cagatacggc gcataagacg cattgctttc ggcgacgttg aacaatgtcg caggctgtac
     2521 atgccccagc aggctgccga aggtgaggaa gaaagtaatg actttcgccc ccagcacaat
     2581 tgctgtcatc cggctgacag ctttcgtact caaccacacc acgaacgcta ccagcagtgc
     2641 aaaaccaaat ccagctgccc gtgcagggac gttcagcgac atctccgcga aggtgtgatg
     2701 cagaatcgaa ccgctggcag aaatataggc gtaagtcagg atatagagca caaaggcaat
     2761 ggaaatgccg ttgactacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
     2821 gctagaaccg attcgataat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
     2881 acagaaccag gtaaagatca gcgccgtcat tgaccagaag aaccacgccc cggacatgac
     2941 cacaggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
     3001 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
     3061 aaaaatagtg cgactgcgtt gttatgtatt gcactgtacc agtacacgag tacaaaagac
     3121 agaaaaaaag ccccgatggt aaaaatcggg gctgtatata ttattttaca gattgtgttc
     3181 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
     3241 acgcggagcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
     3301 ttcacggcgt tcaccgctga agttacgacc gccttcacga ccaccttcac gacgttcgcc
     3361 accgaaacca cgaccaccgc cacgacgctc accgccagta tgcggctgtg catcgcccag
     3421 taactgcatg ttcatcggct tgttgagaat gcgagtacgc gtaaagtgtt gcagcacttc
     3481 acccggcata cctttcggca gttcgatggt ggagtgagaa gcaaacagct tgatgttacc
     3541 gatgtaacgg ctgctgatgt cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc
     3601 aacaccatca tcgcggccca cttcaatgcg gtacagctgc atatcgccaa catcacgacg
     3661 ttcacgacgc ggacgatctt cacggtcacc acgcgggcca cggtcgttac gatcgcgcgg
     3721 accacggtca tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggaac
     3781 aatcagagta cgttcaccct gtgccatttt cagcagtgcg gcggccagag tttcgagatc
     3841 cagctcttcg ccttcggcag tcggctgaat tttgctcagc agtgcacggt attgatccag
     3901 atcgctgctt tccagctgct gctgtacttt agcggcgaat ttttccagac gacgtttgcc
     3961 cagcagttct gcgttcggca gttcaacttc cggaatggtc agcttcatgg tgcgttcaat
     4021 gttacgcagc agacgacgct cacgattctc aacgaacagg agcgcacgac cagcacgacc
     4081 cgcacgaccg gtacgaccga tacggtgaac gtaagactca gaatccatcg ggatatcgta
     4141 gttaactacc aggctgatac gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat
     4201 caggatgtcc agacgaccat ctttcagtcg ttccagtgtc tgctcacgca gcgcctggtt
     4261 catgtcaccg ttcagcgcgg cgctattgta gccgttacgc tcaagagctt ctgccacttc
     4321 cagagtcgcg tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag
     4381 gaaacgtacc agtgcttcgt ttttacgcat accccagaca gtccagtagc tctggctgat
     4441 gtcaggacgg gtagtcacgc tggactgaat gcgcacttcc tgcggctctt tcataaagcg
     4501 gcgggtaata cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc
     4561 ttccgggatc tgcgccataa tagtttcaac gtcttcgatg aagcccatgc gcagcatttc
     4621 gtcagcttca tccagaacca gaccgctcag tttagaaagg tccagagtgc cacgtttcag
     4681 gtgatccagc agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg
     4741 taattgcacg tcataacgct ggccgccgta cagggcaacc acgtttacgc cgcgcatgtg
     4801 tttagagaaa tccgtcattg cctcagcaac ctgtaccgcc agttcgcggg tcggtgccaa
     4861 caccagaatc tgtggtgctt tcagctcagg atcaagattc tgcagcagag gtaaagagaa
     4921 tgctgcagtt tttccgctcc ccgtctgggc catacccagg acgtcgcggc cattcagcag
     4981 atgtggaata cactctgcct gaattggaga tggtttttcg taacccagat cgttaagggc
     5041 ttcaaggata ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta
     5101 gtacgtgtgc ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag
     5161 caattttcat tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc
     5221 ctcacccgtt aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat
     5281 tgctggtccg attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat
     5341 gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc
     5401 gtggcgctgt ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca
     5461 ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct ttccattaac
     5521 gtttgttcgc taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta
     5581 tccgattttt cgaagtgctg tctcaacact tctttagcct gcttctcatc gagcttctgc
     5641 tcggcgagat aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc
     5701 agcagatcat cttgcgctaa cttgtcacga ccgccgtaat ataatgcgat cccgcgattc
     5761 aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca
     5821 gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata
     5881 tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga
     5941 ctatcataca acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc
     6001 cgactggcaa ggatttgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc
     6061 aatggtaccg cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta
     6121 agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca
     6181 cggttgaatg aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc
     6241 cccgccgcag cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc
     6301 ggtgctgcag caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc
     6361 tggcgatcaa cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc
     6421 actttctcaa cgcgtttgtc agcgatctga gagatgtgta ccagaccttc tttaccgccg
     6481 ccgatggcaa caaatgcgcc aaagtcaacg atacgggtca ctttaccatt gtagacgcgg
     6541 cccacttcga tttctgcagt gatctcttcg atacgacgaa tagcatgttt cgctttctcg
     6601 ccgtcggtcg ctgcgatttt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt
     6661 tcttcggtca gagcacggat tacagaaccg cctttaccga taacgtcttt gatcttgtcc
     6721 gggttgatct tgatggtatg gatacgcgga gcgaactcag agatatcgcc acgcggcgcg
     6781 ttgatcgcct gttccattac gcccaggata tgcagacgcg cacctttagc ctggttcagc
     6841 gcaacctgca tgatctcttt ggtgatacct tcaattttga tatccatctg caatgcagag
     6901 ataccgtcgc gggaacccgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc
     6961 aaaatgtcag acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc
     7021 gcaacagcag ctttgattgg cacacctgcg tccatcagcg ccagagacgc gccgcacacg
     7081 gaagccatag aagaggaacc gttggattca gtgatttcag acacaacacg tacggtgtac
     7141 gggaatttgt ccatatccgg catgactgcc agcacaccgc gcttcgccag acgaccgtga
     7201 ccaatttcac gacgcttcgg agaaccgacc atgccggttt cgcctactga gtacggaggg
     7261 aagttgtagt ggaacaggaa ggtgtcagta cgttcgccca ttagttcatc aagaacttgc
     7321 gcgtcacgtg cagtacccag cgttgcggta accagcgcct gagtttcacc acgggtgaac
     7381 agcgcagaac cgtgagtacg cggcagcacg ccagtacgca catccagacc acgaatcata
     7441 tctttttcac gaccgtcaat acgcggttcg cctgccagta cgcggctacg aacaacgttt
     7501 ttctcgatag cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc atcttcagca
     7561 agcagcgtcg cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg
     7621 tcggtgatgc ggtaagcatc gctcagacga gcttcagcca gtgcagcaac gcgcgcgttc
     7681 agcgcttcgt ttaccggctc cggctgccag tcccaacgcg gtttaccggc ttctttcacc
     7741 agttcattga tgttctgaat gacaacctgc tgttgttcat gaccgaacac tactgcgccc
     7801 aacatctggt cttcgctcag cagttcagct tcagattcaa ccatcagtac agcggcttca
     7861 gtaccggcaa caaccagatc cagtttgctc tctttcagct cgtcctgagt cgggttcagt
     7921 acgtactggt cattgatgta acctacgcgg gcagcaccaa tcggaccatt gaacggaata
     7981 ccagacagag acagtgctgc ggaagcgcca atcatcgcga cgatatccgg gttaacctgc
     8041 gggttaacag aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac
     8101 agcgggcgaa tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttggacga
     8161 ccttcacgac ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag
     8221 ttaacggtca gtgggaagaa gtcctgacct ggtttggctt ttttctggcc aacaacggta
     8281 acgaataccg cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc
     8341 atgccggttt ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta
     8401 agcaatgtaa tatcctttct ctttcttaga cagtacctta cggcactggt gttaataccc
     8461 gatcttctgc gcatcctcgc gactaatgac aaccctaacc cagcccaatg tgggtaaagc
     8521 ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt
     8581 ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa
     8641 actcgcaaga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
     8701 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
     8761 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
     8821 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
     8881 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
     8941 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
     9001 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga
     9061 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
     9121 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
     9181 ttccagcggc gcaccggatg tacgaaccgg gttaccattt ttgaagtaaa cagaagacgt
     9241 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
     9301 atcaagtaac tccgcggctg gaatatcctg ctgttcggct tgttcaacca gttcacgcag
     9361 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
     9421 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
     9481 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
     9541 caattcataa acggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
     9601 ttcgtacagt tttttgccct ggtatttcag tgctgaatac atcgaaggga tctgttcgat
     9661 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcactaaagg ttaccggacg
     9721 ttcttcaacg atttgtccgt cggcgtcaga cgtatcggta cgctgtccaa gacgcgcaat
     9781 caccctatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag
     9841 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cagcacggtt
     9901 ggcgttataa atccgtttca ctttttgcag cgcatcgttg ctggacatac cctgcggttt
     9961 atccagcaac aacacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    10021 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    10081 aggtttgaca tgcgcatccc ttcaaccagc gagttgtcgt agaagaaggt cagttccggc
    10141 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcctgc
    10201 agcgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgttacatat
    10261 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    10321 agacgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    10381 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    10441 gccatagccc cttttaaaat taatctcagg tggaaagggc tgtgtaggcc tgataagacg
    10501 cgcgagcgtc gcatcaggca acggctgccg gatgcggcgt aaaagcctta tccggcctac
    10561 aacccagaca accttaagcg atggtacgtt ggatttcgat gatttcgaat acttcgatca
    10621 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
    10681 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
    10741 ccacgttgtc gcgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
    10801 tacaacctgc gatggcacca aatttcggtg atttgaatac gtcacgaact tccgccagac
    10861 cgataatctg ttgtttcagt tccggagaca gcataccgct cattgccgct ttcacttcgt
    10921 cgatcaggtt atagatgacg gagtagtaac gcagatccaa gctttccgct tcaatcactt
    10981 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
    11041 ccagggtagc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
    11101 cttcgtcagt agacagtttc agcaaggagt cggagatagc ttccacagaa ccctgtacgt
    11161 ctgccttaag gacgatattc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
    11221 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
    11281 agagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
    11341 ccggaacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
    11401 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
    11461 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
    11521 tatcgaggaa ggattcgata accgcaccgc tcgccatgcc tttacgtacc gctttcagtt
    11581 ccagaacttc cgcctgcagc aggatagcgt ccagcagctc atcgatacca gtacccgctt
    11641 tcgcagacac atgtacgaac tggctttcac cgccccactc ttccggcaag atgccgtact
    11701 gggagagttc gttcttaacg cgatccggat cggcttctgg tttatcgatc ttgttcactg
    11761 caacgaccac cggaacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    11821 ttacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgag
    11881 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    11941 cagtttcaac gtggtatgca ccaatgtgct gggtaatacc gcccgcttcg ccagaggcca
    12001 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    12061 tggtcacaac cggcgcgcgc ggttcagccg ctgcacccgt gtcacggtcg ctcattaccg
    12121 cttcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    12181 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    12241 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    12301 tggtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg aagccttgct
    12361 gcagcgaaga acctttacgt tttccgccct taccgccacg tactgctgcg cgtgcttctt
    12421 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgca gctttcgcgt
    12481 tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    12541 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    12601 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    12661 cgagtttacg acgcgcttct tcttcggctt tacgcttgag ctctgcagct tcctgctcac
    12721 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    12781 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    12841 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    12901 cttgctcttc cgcttcacgc tgcgcttgct cttccgcagc aaggcgttca gcctcttgcg
    12961 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    13021 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    13081 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcggacacag
    13141 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    13201 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    13261 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    13321 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    13381 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    13441 tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    13501 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    13561 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    13621 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    13681 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    13741 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccattac
    13801 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    13861 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcacg aactgcgccg
    13921 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    13981 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    14041 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    14101 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    14161 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    14221 gaacacggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    14281 cttcagcgtt gttgccaaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    14341 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    14401 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    14461 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    14521 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    14581 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    14641 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    14701 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    14761 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    14821 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    14881 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatgcgg agaaccagag
    14941 tcacctcttc tccgacgaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    15001 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    15061 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    15121 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    15181 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    15241 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    15301 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    15361 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    15421 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    15481 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    15541 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    15601 aaaatctgca ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcac
    15661 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    15721 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    15781 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    15841 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    15901 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    15961 gcgacaaaag ggagctgttc cttatgcata tactgcaaat ctgggccagc cagacgaaga
    16021 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    16081 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    16141 taacaccact ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    16201 caccatgctg gttgctgcta tgaaagaaga tggcgtgaat atctggggtg acggcagcac
    16261 ctataaagga aacgatatcg aacgtttcta tcgttacggt ctgctgacca atgctgaact
    16321 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gtcgtcatga
    16381 aatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    16441 ctccacggac tccaatatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    16501 ctccagcgtc aaaatcgtca acccgattat gggcgtgaag ttctgggatg agagcgtgaa
    16561 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    16621 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    16681 cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa gccgtggtat
    16741 ttacgaggct ccgggaatgg cgctgctgca cattgcgtat gaacgtctgc ttactggtat
    16801 tcacaacgaa gacaccattg agcagtatca tgcacatggt cgtcagttgg gccgtctgct
    16861 gtaccagggg cgttggtttg attcccaggc gctgatgctg cgtgactctc tgcaacgctg
    16921 ggttgccagc cagatcactg gtgaggttac cctggagctg cgccgtggga acgattattc
    16981 gatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    17041 aggtgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaacctgga
    17101 tatcaccgat acccgcgaga aacttttcgg ttatgccaaa actggcctgc tctcctcctc
    17161 tgccgcttcg ggcgtgccgc aggtggagaa tctggaaaat aaaggccagt aattcgacat
    17221 caaccctgcc cttcaggggt ggggttacct tgcttatcct cagtaaactc ctttcttagc
    17281 atcacaaaat cgaaaacatc atggtttgca tctacaaccc gtgagtctcc tttatagtgt
    17341 gcaatcactt cctccagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    17401 ataaggtaat tattgtacgc agtggaaaag atatcgtttt ccgttgtacg atcaacgcca
    17461 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctggcc
    17521 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cgcgttccag gccgtgatcg
    17581 gcaaaataca tgaccgaggc gcgacgatct tttaataatt caaaaacctg acctagcaaa
    17641 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    17701 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    17761 acaatcagtt tcttttgctg cgtcttttgc tgtaacgctt gcgataaatg cggcaacaac
    17821 aattcatcaa atcctctgac atagactgtt tccatggcgc gcatggcgat actggtaact
    17881 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    17941 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacggaa
    18001 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcaccgct tattgcttga
    18061 ttaaacagtt tgatctgttt tctttgtgct tcgacttgcg gtgtcgtaga gcgtgtatat
    18121 ccatacaaag acatattgtc gacgcgtacg gactccccca caatcaacac gtaggtatca
    18181 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    18241 ctttgatgct ctttcgctgc taaagcaaaa taattgagat tgaaaaacgg tgtataggta
    18301 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    18361 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    18421 acttttttcg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    18481 aaaaaggcaa aagcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    18541 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    18601 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    18661 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    18721 agcaatcgct gaaccatatg cgctccggaa gaagcgacta agttcgtaat accaaaaaga
    18781 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    18841 gtcatccaga aatccaagtt cacaaccaat ataaacaatc atcaacaatg ttcaatttta
    18901 tatcagacat gtttttcaac tctgatccat atcagaacga aacactgtta gttttttctt
    18961 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    19021 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    19081 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    19141 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaagtaccag
    19201 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    19261 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    19321 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    19381 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    19441 agctggctga gtttgttcgg ttttcgccgg cgcactcagg ttttcccatt cgctaccttt
    19501 attggttttg ttgctattga tgttacccaa caccagactg atgatgaaga ataacgttgc
    19561 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    19621 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    19681 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    19741 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    19801 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    19861 aattttatcg cattgtatca actgcggaaa aaaacagcaa aactgatgtg ttcgctgaga
    19921 aaaaaggcga caaccgccgc ctttttagcc agttatctca cgctttaaac ggcttttact
    19981 gcatcggcga tgcggtgtgc aaattcagtc acctgtgctt cgtcttcgcc ttccaccatc
    20041 acgcgaatta acggttcggt gccggattta cgcaacagca cacgtccacg gctacccagc
    20101 gccgcttcaa cttctgcggt cacagctttg actgattcat gctccagcgg atcgccgcta
    20161 cctgcggtgt aacgcacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaaga
    20221 tcgtgcaggc tcatatggtt acgcgccatc gccgccagca cctgcaagcc agcaacgata
    20281 ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggagttctc tgcaccgata
    20341 cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
    20401 aatggaatac ctaactgttt cagcgccagt tccagcccca tgttgctcat caatgtgccc
    20461 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    20521 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg
    20581 tcgaaggcaa taccgagatc cgctttttca gccagcactc gcgcctgaag cgcgcgaacg
    20641 tcggtagccc ccacttcggc attgatgttc acaccgttcg gctcacaacc gatagcgata
    20701 acattcgccc ctagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accatttgca
    20761 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    20821 aactcgatat agcgacccgc agcatcaacg atacggctgg ctttacccag ttctgccgaa
    20881 tcaacacagc tgatctcttt ttccatttcc gcttcgatgg cctcttctac tgcatccggt
    20941 agtttggtgc cgtcgataga gaaaaattta atgccgttat cgtagaacgg gttatgcgat
    21001 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    21061 gttggcatcg ggccagtgaa gagcgcggaa aggcccgcag ccgccagacc cgcttccagt
    21121 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataatgat cttacgggag
    21181 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggggtg
    21241 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    21301 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    21361 ttctttgacg tcatgaacac gaatgatatg cgcgccttgc atagcggcaa tgaccgcaca
    21421 agccagacta ccgctcagac gctcagatgg ccccacgttc agcaattgcc caatcatcga
    21481 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    21541 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    21601 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa aatagcgatt
    21661 cacttccgca aagacatcgt catacttcgg ggcttcctgc atggtctttg gatttccctg
    21721 catatgcatc aggcaaaccg gcaaaccggt ttctgcagca gcctccagag cgcctggttc
    21781 ggaaagggag cgaatatcgt taataatgtg agcgccaact ttcgctgact cacggatgac
    21841 ttctggtttg gatgtatcta ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    21901 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ccgggcgtgt
    21961 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcgttg atcatcagat tcgcatgttt
    22021 caccgcatct atcagcgagt tatgcgtgcc gccatccgaa aaggaatcag gcgtgacgtt
    22081 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtgccctggg caaagagttt
    22141 catggtgtta tccctggtat aaattgataa gaaaaaaccc cggagcacgc cccggggttt
    22201 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    22261 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gtggagcctt tggactacca
    22321 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    22381 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    22441 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    22501 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    22561 tgtttcgctt tcgctacgct acggccgagg aacacttcac cttcttcttc cgcgtacagc
    22621 agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc
    22681 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta aatgatctct
    22741 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    22801 ctgattgcgt caccctcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    22861 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcgtgaccc
    22921 gcttcgtggt aagccgtcga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    22981 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    23041 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    23101 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga
    23161 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaaacc aaccacaacc
    23221 tgacggtcga aacggccagg acgcagcagc gccgggtcga gaacgtccgg acggttagtc
    23281 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    23341 ttcagagtct gttcacgttc atcgtgacca ccgcccaggc ctgcgccacg ctggcggcct
    23401 acggcgtcga tttcatcgat aaagatgata cacggtgccg ctttcttcgc ctgttcgaac
    23461 atgtcacgaa cacgggatgc acccacaccg acgaacattt cgacgaagtc agaaccggag
    23521 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcag cgttttaccg
    23581 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    23641 cggctcggct cgcgcagata ctcaaccagt tcagcaactt cttcttttgc ttcgtcgcag
    23701 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    23761 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    23821 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    23881 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    23941 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    24001 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    24061 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    24121 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    24181 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    24241 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    24301 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    24361 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    24421 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    24481 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    24541 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    24601 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    24661 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    24721 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    24781 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    24841 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    24901 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    24961 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    25021 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    25081 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    25141 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    25201 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    25261 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    25321 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    25381 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    25441 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    25501 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    25561 gccacgagca ataggtgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    25621 gccaacgatg cgataagtct gttcttcgtc agaatcgaga ttcagcaccg ttacggtagc
    25681 accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    25741 cagcttggct tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc
    25801 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    25861 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    25921 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    25981 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    26041 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    26101 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    26161 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    26221 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    26281 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    26341 tattgattac cacagtcagc agatggcgct gcccgccagt acccagaaag tgattactgc
    26401 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    26461 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    26521 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    26581 caaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    26641 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ccgccatagt
    26701 tgaccgcaac tgtttctccg tctcgctcta cagtgcccaa aaacccggtg atatggcttt
    26761 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    26821 tgggtctgcc gaagcgcaat attgcgagct ggatgtggtg ccaggcgacc tgaaccgctt
    26881 tacgctgacc ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    26941 ggatggtgcc agctatgctg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    27001 ctggagcgga acactgctgc gtcagacgca ggttaacgaa cctggaacgg tagttgccag
    27061 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cagacaacat
    27121 gatcgccgat acggttttcc gcatgatagg ccatgcacgc ttcaatgtgc ctggaacatg
    27181 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagctggtg tcgatattgg
    27241 taacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    27301 catgatgcag gtgctgcaat acatagccca acacgacaat gaacttaact ttatctctat
    27361 gctgccgctg gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    27421 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    27481 attcattacc acagcgagcg ggcaacgaat ggcgtttgtg caatatcttt ctggctatgc
    27541 agtagaaccg gcggatcagc gtaatcgccg tattccgttg gtacgttttg aaagccgttt
    27601 gtataaagat atttatcaga acaattagtc aataagaaac cccggcacat ggccggggtt
    27661 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcgt
    27721 catcccagtc ttcatcatcc tcttcagcaa tctcttcaag ctgctgacgg tgataatcat
    27781 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    27841 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtccg ctcgccgcag
    27901 agatcagata atatttatct tcccaaccca gcgcctcagc gatcgctttc gctttctctt
    27961 cggcttctgc cttatccagc aggtcgatct tgttgaagac taaccaacgc ggtttcgctg
    28021 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    28081 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    28141 gcttcaggaa gcgaatgccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    28201 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    28261 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    28321 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    28381 gcagcagctc acgcttatcg cccggcgtac cgttagtttt ctgccgtggt gtacggttaa
    28441 ctgacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    28501 gctgaccgtg tttggtcata tcgcccatgg tttcaccagt accctggtcg attacacgcg
    28561 tacctaccgg cactttaatc gtgacgtctt taccgcgctt accggtacag tcgcggcttg
    28621 cgccgttctg accacgctct gcacggaaag atttttcaaa acgataatct ataagcgtgt
    28681 tcaggttctc gtcggcttcc atccatacgt caccaccatc accgccatcg ccgccatccg
    28741 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    28801 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    28861 cgcctgcgcg cggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    28921 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    28981 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    29041 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    29101 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    29161 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    29221 caattgtaca taaagtgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    29281 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    29341 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    29401 tcaccagacc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    29461 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    29521 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    29581 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    29641 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    29701 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    29761 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    29821 taccgtattc cctgcaaagc gcatttgcga attaacgtcg caccatgcgc atttttaccg
    29881 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    29941 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    30001 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    30061 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    30121 cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg
    30181 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    30241 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    30301 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    30361 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    30421 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    30481 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    30541 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    30601 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    30661 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcaccc
    30721 tatattccta accataaacc ctaacttgcc tttgttcaca gtaaggtaat cggggcgaaa
    30781 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    30841 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccaactgat caatcagtta
    30901 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgcc cgatgattgc tgtactggct
    30961 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    31021 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    31081 aaagctaccg ccaacgccgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    31141 tatacccgcg cgttccagat gatgaccagc ctcggctcgc tcaaagtgct ggaagtcatg
    31201 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    31261 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    31321 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggagga ggagaaaggc
    31381 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    31441 tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg acgatctgaa cgaaggtaaa
    31501 ccgacgctgc cgctgctgca tgcaatgcat catggcacac cagaacaggc acagatgatt
    31561 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    31621 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    31681 atcgcagcgt tacaggtgct cccggacacc ccgtggcgag aagcactcat cggcctcgcg
    31741 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcgggat
    31801 gattccagta aattccataa aaacacttat tcagctctat aacacctgaa aatagggctg
    31861 tacatcttta tatattctga atattcacac tctttacagg aactttttag agcaataggc
    31921 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    31981 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    32041 tttgcgcaag aagggaacgt caatggcggc ggaatctcgc agaaatggtt tgagttcctc
    32101 aacgctggcg aacgcattat cgcgcccctg gccgaaagga gagatgatta ttgcgaaagc
    32161 cctgggaact gacccctgga ttatctggcc atctcgctac catgatccgc tgacccatga
    32221 gtttatcgac agaacgcagt tgatacgtag ctacactaaa ccgaaaaaat gagtggtctg
    32281 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    32341 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    32401 ataaatacga tcaactaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    32461 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    32521 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctcagct ctggcacatg
    32581 cataaagcgg ttttcaaaga ccgtttcggt gatgaagccg gtcccttctg ccaccaggtt
    32641 caacagcgtg aactgggcct gcatatcagt cgggaatgcc ggatgcggcg cggtacgtac
    32701 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    32761 gatgtctgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    32821 gttacggcag ataattttgc cgcgagagat cgccgccgcc accaggaaag taccggtttc
    32881 gatacgatcc ggcagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    32941 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    33001 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    33061 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    33121 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    33181 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgtgcaccga tcgtacaacc
    33241 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gcgcccagat
    33301 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    33361 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    33421 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    33481 tagtgcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    33541 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    33601 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    33661 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    33721 atattccatc agcggcccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    33781 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    33841 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    33901 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    33961 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgt
    34021 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    34081 aatttcgcca gggtatacac tttatcgttt accccctgaa gcgtcacgtt gttgccctgc
    34141 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    34201 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    34261 ggtagcagaa cgtcctgatc cagctctccg gatagcgcca gcgtgtcacc cgtctgcatc
    34321 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    34381 atttcagttg tgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    34441 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagttgc
    34501 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcgggcgg ccattcgggt
    34561 caataatggt aacgcgaata ggcacaatgg ttttatcgcc tagcggctgt tctggcgcaa
    34621 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    34681 cacggaaagc ggcaaagtaa gcatcacgtt gagcaggggt cgcactcttg taatactggc
    34741 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcgacaa
    34801 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    34861 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    34921 ccgcactcag cggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    34981 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    35041 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    35101 gaccaataag atcttccagc accatcgcag acttggtgtc ctgaattgta tcgccatcct
    35161 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    35221 cccccagcag gccggaagta cgaatgctca gcgaactggt atcaggaatg tggttataac
    35281 gttgttcaat ttccagcgtt acgcgcggca gataggtttt cgggtccagc gtaatatccg
    35341 ccacccggcc cacaacaaca ccaccaatgc tgaccggaga gcgcgctttc aggccgccaa
    35401 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    35461 ccgccttcag gcaaacaaac agcgccgcca gtagtgctgc taataaaaag atacccaccc
    35521 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    35581 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    35641 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    35701 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    35761 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    35821 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    35881 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    35941 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    36001 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    36061 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    36121 cccaacacca ttccgatgaa cacgccagaa actacaataa tcagcatcga caggacgccg
    36181 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    36241 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    36301 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    36361 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    36421 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    36481 aatttttttg tccgccagga tccaggcgtg atcggcaata cttaacactt ccggcacatc
    36541 gtgagaaacc accacacaag tcacacccag cgcgctgttc agctcagaaa tcagcttcac
    36601 cagtacgccc atggtgatgg gatcttgccc aacaaagggc tcatcaaaca tgatgagatc
    36661 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cggaaagttc
    36721 agaaggcatt agtttagccg ctccacgcag ccccacggcc tccagcttca tcatcaccgt
    36781 actatgcaac aatggcgcgg gaagttgagt atgttcgcgc agtggatagg cgacgttgtc
    36841 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    36901 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaagaatctc
    36961 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    37021 cgatggcccc atgatcgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    37081 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    37141 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    37201 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    37261 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    37321 cagcggaccg gcgagtatac ctgaagaaag gacgttagat gcttttagct acggcactgt
    37381 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    37441 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    37501 gtacatcgtt accagaaatc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    37561 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    37621 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    37681 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg
    37741 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    37801 gtcaggctga acgtcagggg actgacagtc tgaccagaga gcagcttgca gagctgccgc
    37861 gtgaaggtgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    37921 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    37981 agttgacgat ggggctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    38041 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaacatc attggcgcaa
    38101 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    38161 atccactggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    38221 tgctgtgctg gcggcgctcc ccgcaatcgg gccgtggtgt aggggtatta ttaactggcg
    38281 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    38341 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    38401 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    38461 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    38521 gggaaatcgg ggcatattgg gcgcaaaatg gcggcaacgt ttgccagcac cggtacacct
    38581 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    38641 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    38701 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    38761 gcgcgcgccg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    38821 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    38881 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacatcc aggcggcgca
    38941 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    39001 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    39061 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    39121 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtatttc cgatgttatg
    39181 acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    39241 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    39301 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    39361 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatagcaa
    39421 aagcagagaa cattcgtctg ctgatcctcg atgtcgatgg cgtactgtca gatggcctga
    39481 tttatatggg caataatggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    39541 ttcgttgtgc gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    39601 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    39661 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    39721 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    39781 tggccgatgc gcatccactg ttgctcccgc gcgccgatta cgtgacgcgc attgctggcg
    39841 gtcgtggtgc agtgcgtgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    39901 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    39961 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    40021 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    40081 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    40141 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    40201 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    40261 cgttgaagtc aacgcacttg tgccggactc tcaacttcgc agaatcacga cggataacgc
    40321 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    40381 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    40441 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    40501 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    40561 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    40621 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    40681 tcgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    40741 caacgttcta ccagatgcag gacaatggta aacccgttga aggtcacgct tcccagatgc
    40801 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggtcg
    40861 atagcaacat taagggcgat aagatcactt acctggtgaa agagcagaaa atgcaggctt
    40921 tcagcgacaa aggtaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggacaaaa
    40981 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    41041 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    41101 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    41161 ctttctacat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    41221 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    41281 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    41341 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    41401 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    41461 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    41521 ttgccggggt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    41581 acagcggcct gggcgtactg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    41641 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    41701 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    41761 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    41821 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    41881 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    41941 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacact
    42001 ggacaccgcc gacgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    42061 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    42121 cgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    42181 ggtcgagctg acaccgtttt ccgatactga ccgcgccatt gctacctcta tcgtcgatgc
    42241 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    42301 tgaagagatc gacatcgacg aagttgaagc cgtccttaag cggatccaac ggtttgatcc
    42361 ggtcggtgtg gcggcaaaag atctgcgtga ctgcctgctg atccaactct cccaattcga
    42421 taaaaccacg ccgtggctgg aagaggccag actgattatt agcgatcatc tcgatctgtt
    42481 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    42541 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagacggg
    42601 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacggt
    42661 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    42721 caataacgcg cgcaacgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    42781 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    42841 cgttgaacag cagcaagcct tctttgagca aggcgaagaa tatatgaaac cgatggtact
    42901 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    42961 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    43021 caataccgag ggcggcggcg aagcctcctc cacggcgatt cgtgcgctgg tgaagaaatt
    43081 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    43141 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    43201 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    43261 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    43321 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    43381 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    43441 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    43501 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    43561 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    43621 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    43681 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    43741 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agtctgccgc
    43801 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    43861 atggtattgc cattccgcat ggcaaactgg aagaagatac tcttcgcgcc gttggcgttt
    43921 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggatttac
    43981 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcactgg
    44041 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    44101 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    44161 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    44221 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    44281 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg
    44341 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    44401 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actcctgttc ctggatgccg
    44461 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    44521 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    44581 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gcagaaatgc
    44641 tgcgtacccg tctgctgggt aaacgtgaac gtgaactgac catggtcttt gagtctttcg
    44701 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    44761 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataagccc gtcgccgcgt
    44821 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgagc
    44881 tatggttacc tatgctggaa accaacaacc gtagctacct gacggtcgcc attggttgta
    44941 ccggcgggaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    45001 gcggtaaaaa tgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    45061 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    45121 tgaattaatg caaggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    45181 tgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga
    45241 agttgaagcg accggtccac aggaagagga agcactggcc gccgttatcg ccctctttaa
    45301 ctctggtttt gatgaagatt aatcttcatc actttgacat tcaaatccct tcaagctccc
    45361 ccggcaataa gaaaagcgcg tactgaaatc gttctcaatc aacgtcaatt gtacatttcg
    45421 tgcgcttttt actttcagaa gaaccttaag aaaaccttaa gaagcattgt ttaggtttcg
    45481 tttaagttaa tcgaccatac tggagatcgt cagaaaatat ttccaggaga tggcatgatt
    45541 cgcttatctg aacaaagtcc cctgggcacc ggaagacatc ggaaatgtta tgcgcatccg
    45601 gaagatgccc aacgctgtat caagattgtc taccatcgtg gcgatggtgg cgataaagag
    45661 atccgccgtg agttaaagta ctacgcgcat cttggtcgcc ggttaaaaga ctggagtgga
    45721 ataccgcgct atcacggtac cgttgagacg gattgcggaa ccggttatgt ctacgatgtg
    45781 attgcagatt ttgacggcaa accctccatt acgctgaccg aatttgctga acaatgccgt
    45841 tacgaagaag acattgcaca actgcgccag atactgaaac agctgaagcg ttatttgcag
    45901 gataaccgta tcgtgacgat gtcgctgaag ccgcagaata tcctctgtca ccgcatcagt
    45961 gagtcagagg ttatccccgt cgtctgcgac aacattggtg aaagcacatt gattccactg
    46021 gcgacctggt cgaagtggtg ttgtctgcgt aagcaagagc ggttatggaa acgatttatc
    46081 gcccaaccgg cgctggctat tgccctgcag aaagatctgc aacctcgtga gagcaaaacg
    46141 ctggccctca cttcgcgcga agcttaatca agctggtgct gttgcataaa cgactcaccg
    46201 cctaactgat acatctgccg taaaatccac gcctgacggc cacgcacgta gcccgatggc
    46261 gcggagactt tgaaacgaag tggattaggt aatacagctg ccagtaatgc agcttccgag
    46321 cgggtaagtt tgctcgcggg tttgtggaaa taacgttgtg ccgcagcttc gacgccaaac
    46381 acaccgtcgc caaattcggc gatattcagg taaaccgtca ggatacgctt tttgctccag
    46441 acagtttcta tccccagcgt taatccagct tccagccctt ttcgaaccca gctacgccca
    46501 tcccataaaa agagattttt ggctgtctgc tgagaaatcg ttgacgcacc gcgaatacgg
    46561 ttttcattgc gctcgttgtg ggccagggct tgctcaatgg aggcgacatc aaagccccag
    46621 tgctcaggaa acttctgatc ttctgcggca atcaccgcca gtcccatcca cggcgaaatt
    46681 tgatccatac tgacccagtc agaatgtgcc acgtaacgaa aattgccatg cagccaggcg
    46741 ctgacttgtc gctcggccat taccgctgag aaaggaacag gcgcaacgct aaacaacgcg
    46801 atgcccccgc cccagaaaac ggcgaggaca accattaacc gcagaagaaa acggcgaacg
    46861 aaactaaaca ccgttaagcg gcttttactc attcagccag aaccagcacg cgggaaacca
    46921 gcttatcaat gccgctcgcc gcttctgcaa tgttctgcgc cagcatatat gccggggtgg
    46981 tgacaatctt attgtcttca tcaaccacga tatcatcgac aggacacggc acatgctccg
    47041 cgcccatctc ttccagcact tctgcggtat cgatatcagt accgatggtc aaacgcagcg
    47101 ggaaatcgaa aattttcggc agcatcgccg gggcaataca cataaaacca agcggttttc
    47161 cggcctgatg catcgcttgt gccagcgcct ttaattcacg gtcaacggtg cattcgctac
    47221 caagactggc aaaattgctt aaattcttcg ccgcgccaaa accccccggc acaatcaacg
    47281 catccagttc agcggcatcg gcctgggcca gaggacggat ttcaccgcgc gttattcgtg
    47341 ccgcttcaat cagcacattg cgcgtttccg tcatcgcttc gcccgttaaa tggttgataa
    47401 catcaacctg ctgcttatcc ggtgcaaagc agaccgcctg cgcaccgctg cgtgaaatag
    47461 ctaacaacgt caacaccgct tcatgaattt cagaaccgtc atagacgccg catccgctca
    47521 gaattacgcc aattttcttc attgtgatca tcctttcgca attgactgaa acacattaat
    47581 ttttttaata aaaatgctac gcatcacaca tttaactgat tcatgtaaca aatcatttaa
    47641 gttttgctat cttaactgcg tgcagcctga aaaacagtgc tgtgcccttg taactcatca
    47701 taataattta cggcgcagcc aagatttccc tggtgttggc gcagtattcg cgcaccccgg
    47761 tctagccggg gtcatttttt agtggctttt gccacccacg ctttcagtac ttctacgtcg
    47821 tgacgccact cttctttcat ctcttcaatc cattcaccga cgttatcttc ccaggcggga
    47881 aggtcaggag actgaatttg ctgacccagc tgttgcagat ggcgtaaccc cactgacccc
    47941 gccgcacctt taattttatg tccttcctca acaatgcctt ttttatcctg cgccgtcaga
    48001 ttcgactcca gcacgctgac atagcccggc atcatcttct caaacaccgc taacccgtcg
    48061 gtgatcagct tcggtcctac aagttcgaga tactgttcca gcatgggaat atcgagcaat
    48121 gcttctgatt tactgttctc ttctgtcgtc accgtacttt cctcatcatc ctgggtatcc
    48181 cagaatttct tgatcatcgc ggttagcgcc ggaacagaaa gcggcttact cagcacatca
    48241 tccattccag cattgaggta ctcttgcttg tctttcagca cgttagcggt taaggccacc
    48301 agcggcggta aatcctcgcg cggataacgc ttcgtcagtt cacgagagat atccagcccg
    48361 gtcatatctg gcaactgaat atccagcaac accaggtcgt attcgcccgg tttaaacatt
    48421 tccagcgccg ccttgccggt catggcgaca tcaacgctgt tacctaattt ttccagcaca
    48481 gagcgtgcga caatcacgtt cagttcaata tcttccacca gcagcacatt cagcgccggt
    48541 aaaggcatat cgtcttcatc aaacgcatca tcgacctctt cagccaccga cggtgcgtgg
    48601 atcgtcaacg taaaggttga acctttgccc tgttcgctgg taaccgtaat atcgccgccc
    48661 atatttttcg ccagacgacg agaaacggcc agaccaatac cggtgccggt ggcaggttta
    48721 ccgccatgac tgtctttcac ctggtaatac atggcaaaga ttttatccag ctcatcctgc
    48781 ggaatgccga taccagagtc ttccacttca aaatgcagca tatcgccttc atcgtagcgc
    48841 acgcgcacgg taacctggcc ttgctgggtg aatttgacgg cgttactgat gagattccac
    48901 aggatctgcc gtaaacgcgt cccgtcggta atgacctgat gcggtaatgg cagcgtaggc
    48961 tccagattaa agcgcaatcc tttttgttgc gcctgcaatg cggagagatt ttccagatcg
    49021 gcaaggaagc tggtgaaatc aaccggctga ttatcaagct ggaccttacg ccgttccatc
    49081 ttatccatgt cgataatatc gttgaagata ttcccgagcg tgacggccga aacatggatg
    49141 gttttgagat atttttcctg ctcggcggtg agttcggtat ccagcagaat gcggctcagg
    49201 ccgacgatac cattcagcgg tgtacgcaat tcgtgactga tggtggaaat aaacgtcgtt
    49261 ttgtcgcggc tggcgcgttc aagcgcatcc tgataccgct tacgctcggt aatgtcgcga
    49321 ccaaaaccca tcaaaccgtg acgtttaccc acgcggtcgt agtacggcac tttacggatt
    49381 tcaaaacagg ctttgcgccc gtccgggtaa tccagccact gttcataggt cagtgacaca
    49441 ttatgacgga acactttttc atcggtttca atgactttcg cagcggcttc cggtgagtaa
    49501 acatcagcag gtttcaggtg aaccagttgt ttttcgcttt ttccggtcag cagctccatc
    49561 gcgcggttac agccggaaaa ctctttatct tcgttacgat aaaaaaccag gtcgggtgaa
    49621 gcgtcaagga aggaacgtaa gaatgaggat tgctgctcga gctgaatttg tgtctcttcg
    49681 cgctctttga tttcaatttt cagttggccg aaggtttcct gcagttctgc ttccgctttt
    49741 tcacgaacgg cgatttcctg atttagctgg gcaatattat cttttaactg aacgttgagg
    49801 ctcaaatcgc gctcgcgcat ctcctccagt ttttgcacca gtcgtgacag acgttgtcgt
    49861 gactcctcca gttgctcgac gaccaccgat agaaagtaga ccgcccacgg cgtaatcagc
    49921 aaaccaaaga agatagaacg aataacatca atgctttcga cctgaccatg cagcaccatg
    49981 gttaccgcca tttgtaccac aatggcaaga acgacgaggg ccagcgccag caacattgag
    50041 aagcgcacca gacctaactt catcatcagg tcaacataat actgcgccag cagacgaatt
    50101 tgcttcatta gggaattcct tcacgacaac ctgccccaat aatacccaat tctgacagtt
    50161 acgttataaa ttgtgtgaga aatgcggaat tcccctcaat ggcctactcc gttggaggta
    50221 gccacggacg tcccagcgcc gatccctgta cgccatgttc attcagatag cgatccagct
    50281 cgaccattcc cgtccatcga ttttcgcacc acagaggagc aagcagcgtc gggcgacggg
    50341 cgctggcgga aatgcggtgg tagatcactt ccggtggcgt atggcgaatc atttctccgg
    50401 cagtgagcgt gtaatcttcc agttcaatac cgttcaaacg ccccgcttcc caggctttcg
    50461 ccataatgct gcctttcaca atatgcagcg gatgcagctt tatgccgtcc acgccagttt
    50521 caaccactcg ctccagcgtt tgcaaacatt cagcctgccc ttcgccaggc aggccgacaa
    50581 ttaagtggga acacaccttc agcccgcgct gacgtgccag ctgggttgta cgctgataac
    50641 aggcaaaatc atggccgcgg ttgatgcgat gtagcgtttt gtcgtgggcg gtttgtagcc
    50701 ccagctccag ccacacttcg tagccctggt ctttatattc acaaagcaga tccagcactg
    50761 catccggcac gcagtccggg cgggtaccaa cacacaaacc gacaatattg gcctggctca
    50821 ccgcctgctg atacatagaa cgcagcacct gaacttccgc aaaggtgctg gtatacgcct
    50881 gaaagtaggc cagatagcgt ttagcgcggt taactaaatt cgcctggtgc gccagttgtt
    50941 cggcaataga acgatgttgc tgcgcttcat cggcaaacga ggcaacatta cagaatgtgc
    51001 agccgccacg cccgatggta ccgtcacggt tagggcagct aaaaccgccg tgcagcgtca
    51061 gcttatgcac cttttgccca taacgacggg tgagatcacc accaaacata ttgactaatt
    51121 tctgtaactg cataatctga tagaccgcgc cttgaaaaga ggccaaagcc tgccattttt
    51181 agctcaattc ggcgatgacc tggatcaatc gtcccggcct gcttttatca actgcataat
    51241 caatcaaaat taccgaaatt tcatgcatga tcacataaat catttttgct tatcttgtgt
    51301 cagatttttt tatctcctta tagattttag gcaaaaacag tagcataaaa tgccattacc
    51361 aattaaggca gtataaaatg ctggttttct cgtcagttca aggcaggata agggttaaca
    51421 cgcctttatg acagtcagga attggctgtt tctctaccga cgcccctttt agccttaaat
    51481 agaaaatcca ctttaatttc agcaaattaa taaagaattt tgcgctaaag cacatttctg
    51541 taccaataag cttgccattt gacctgtatc atctttcccg ataagttgga aatccgctgg
    51601 aagctttctg gatgagcagc ctgctcatca tatttatgca gtaattgaga tcccctcttc
    51661 accgtattaa ccgatgcgaa aaggacaaca agggggcgaa tgcgaggcgc gcgtatgaca
    51721 cgcaaacccc gtcgccacgc tctttctgtg cccgtgcgca gcggttcgga agtggggttc
    51781 ccgcagagcc tgggggaggt tcacgatatg ttgtacgata aatcccttga gagggataac
    51841 tgtggtttcg gcctgatcgc ccatatagaa ggcgaaccta gccacaaggt agtgcgtact
    51901 gcaatacacg cactggcccg catgcagcac cgtggcgcga ttctcgccga tggtaaaacc
    51961 ggtgacggtt gcggcttgct gttacaaaaa ccggatcgct tttttcgcat cgttgcgcag
    52021 gagcgcggtt ggcgtttagc gaaaaactac gccgtcggga tgctcttcct gaataaagat
    52081 cctgaactcg ccgctgccgc acgccgcatc gttgaagaag agctgcaacg cgaaacctta
    52141 tcgattgtgg gctggcgtga tgtccccacc aacgaaggcg tgctgggtga aatcgccctc
    52201 tcctctctgc cacgcattga gcaaattttt gtgaacgccc cggcaggctg gcgtccgcgc
    52261 gatatggagc gccgtctgtt tatcgcccgc cgccgcattg aaaagcgtct cgaagccgac
    52321 aaagacttct acgtctgtag cctgtcgaat ctggtgaaca tctataaagg tctgtgtatg
    52381 ccggcggatc tgccgcgctt ttatctggat cttgcggacc tgcgtctgga atcggccatt
    52441 tgcctgttcc accagcgctt ctccactaac accgtaccgc gctggccgct ggcgcagccg
    52501 ttccgctacc tggcgcataa cggtgaaatc aacaccatca ccggtaaccg ccaatgggcg
    52561 cgtgcgcgta cctataaatt ccagacgccg ctgatccctg acctgcacga cgccgcgccg
    52621 ttcgtcaacg aaaccggttc tgactccagt tccatggata acatgctgga actgctgctg
    52681 gcaggcggga tggatatcat ccgcgccatg cgtctgttag taccacccgc ctggcagaac
    52741 aacccggata tggacccgga actgcgtgcc ttctttgact ttaactccat gcatatggag
    52801 ccgtgggatg gcccggcggg catcgtgatg tctgacggtc gttttgccgc ctgtaacctc
    52861 gaccgtaacg gtctgcgtcc ggcgcgctac gtcatcacca aagataagct catcacctgc
    52921 gcctctgaag tcggtatctg ggattaccag cctgacgaag tggtcgaaaa aggccgcgtc
    52981 gggccaggcg aactgatggt tatcgacacc cgcagtgggc gtattctgca ctcggcagaa
    53041 accgatgacg atctgaaaag ccgccaccct tataaagagt ggatggagaa aaacgtccgc
    53101 cgtctggtac cgtttgaaga tctgcccgat gaagaagtgg gtagccgcga actggacgac
    53161 gacacgcttg ccagctatca gaaacagttt aactacagcg cggaagagct ggactccgta
    53221 attcgcgtac tgggcgaaaa cggtcaggaa gcggtcggtt cgatgggtga cgacacccca
    53281 ttcgccgtgc tctccagtca gccgcgcatt atttacgact acttccgcca gcagtttgcc
    53341 caggtgacta acccgccaat cgacccgctg cgtgaagcgc atgttatgtc gctcgccacc
    53401 agtatcggtc gtgaaatgaa cgtcttttgc gaagcagagg gccaggcgca ccgtttaagc
    53461 tttaaatcgc cgattctgct ctactccgat ttcaaacagc tcacgacgat gaaagaggag
    53521 cactaccgcg cagatacact ggatatcacc tttgacgtca ctaaaaccac gctcgaagcg
    53581 acggtcaaag agctgtgcga caaagccgaa aaaatggtac gtagcggcac tgtgctgctg
    53641 gtgctctccg accggaatat cgctaaagat cgcctgccgg ttccggctcc gatggcggtt
    53701 ggcgcgatcc agacccgtct ggtcgatcaa agcttgcgtt gcgatgccaa catcatcgtc
    53761 gaaaccgcca gcgcccgcga tccgcaccat ttcgccgtgt tgctgggctt cggtgcgacg
    53821 gctatttatc cgtaccttgc ctatgaaacg ctgggccgcc tggtagacac ccacgcgatt
    53881 gccaaagatt atcgtaccgt gatgctcaac taccgtaacg gcatcaacaa aggcttgtac
    53941 aaaatcatgt ccaaaatggg catctccacc atcgcctctt accgctgctc gaaactgttt
    54001 gaagcggtcg gtctgcacga tgatgtcgta ggcctgtgct tccagggcgc tgttagccgc
    54061 attggtgggg caagctttga agacttccag caggatctgc tgaacctgtc gaaacgtgcc
    54121 tggctggcgc gtaagcccat cagccagggc ggcctgctga aatacgtgca cggtggcgaa
    54181 taccacgcct acaacccgga cgtggtgcgc acactgcaac aggcggtaca aagcggcgag
    54241 tacagcgact atcaggaata cgcgaagctg gttaatgagc gtccggcaac cacgctgcgc
    54301 gatctgctgg caattacgcc gggtgaaaac gcggtcaaca ttgctgatgt tgaaccggca
    54361 agcgaactgt ttaaacgctt cgataccgct gcgatgtcta tcggcgcgtt aagcccggaa
    54421 gcccacgagg cgctggctga agcgatgaac agcatcggcg gcaactctaa ctccggtgaa
    54481 ggcggcgaag atccggcacg ctacggcacc aacaaagtgt cgcgcatcaa gcaggtggct
    54541 tccggtcgct ttggggttac tccggcgtat ctggtcaatg ccgacgtcat tcagattaaa
    54601 gtcgcccagg gcgcgaagcc gggcgaaggc ggtcagttac cgggcgataa agtcacgcct
    54661 tacatcgcca aactgcgcta ttcggtgccc ggagtgacgc tgatctcccc gccgccgcac
    54721 catgatatct actctatcga ggacttagcg cagcttattt tcgacctcaa gcaggttaac
    54781 ccgaaagcga tgatctccgt gaagctggtt tccgaaccgg gagtaggcac catcgcgact
    54841 ggcgtggcga aagcttatgc cgatttaatc accatcgcag gctatgatgg cggcaccggc
    54901 gcaagtccgc tttcatcggt gaaatacgca ggctgtccgt gggaactcgg cctggtggaa
    54961 acccaacagg cgctggttgc caacggttta cgtcataaaa ttcgtttgca ggttgatggc
    55021 ggcctgaaaa cgggcgttga tatcatcaaa gcggcgattc tcggcgcaga aagcttcggc
    55081 ttcggcactg gcccaatggt cgcgctcggt tgtaaatatc tgcgaatttg ccacctgaac
    55141 aactgcgcca cgggtgtagc aactcaggat gacaaactgc gtaagaatca ctatcacggc
    55201 ctgccgttca aagtgacgaa ttactttgag tttatcgccc gtgaaacccg cgagctgatg
    55261 gcacagctgg gggtaacgcg gctggtggat ctgattggtc gcaccgacct gctgaaagag
    55321 ctggacggtt tcaccgccaa acagcagaaa ctggcgctgt cgaaactgct ggagaccgcc
    55381 gagccgcatc ccggtaaggc gctctactgc accgaaaaca acccgccgtt tgataacggc
    55441 ctgctgaacg cgcagttgct gcaacaggcg aaaccgtttg tcgatgagcg ccagagcaaa
    55501 accttctggt tcgatattcg caacaccgac cgttctgtcg gcgcgtcgct ttcaggctat
    55561 atcgcccaga cgcacggcga tcagggtctg gcagccgatc cgatcaaagc gtacttcaac
    55621 ggcaccgcag gccagagctt cggtgtgtgg aacgcgggcg gcgtggaact gtacctgacc
    55681 ggtgatgcca acgactatgt cggtaaaggc atggcgggcg gcttaatcgc cattcgtcct
    55741 ccggttggtt cagccttccg cagccacgaa gcaagcatta tcggcaacac ctgcctgtat
    55801 ggcgcgaccg gtggtcgtct gtatgccgca ggccgcgcgg gtgaacgttt cggcgtgcgt
    55861 aactccggtg ctatcaccgt ggtagaaggc attggcgaca acggctgtga atatatgacg
    55921 ggcggtattg tctgtattct gggtaaaacc ggcgttaact tcggcgctgg catgactggc
    55981 ggcttcgctt acgttctcga tgaaagcggc gatttccgca aacgcgttaa cccggaactg
    56041 gtcgaggtct taagcgttga cgatttggcg atccatgaag agcatctgcg tggacttatc
    56101 accgagcatg ttcagtatac tggctctcag cgcggtgaag agattctggc gaactggtca
    56161 accttcgcca ctaaatttgc gctggttaaa ccgaagtcca gtgatgtaaa agcactgctg
    56221 ggtcaccgta gtcgtagcgc agcagagttg cgcgtgcagg cgcagtaagg ggtagcaaca
    56281 atgagtcaga atgtttatca atttatcgac ctgcagcgcg ttgatccgcc aaagaaaccg
    56341 ctgaagatcc gcaaaattga gtttgttgaa atttacgagc cgttttccga aggccaggcc
    56401 aaagcgcagg ctgaccgctg cctgtcgtgc ggcaacccat actgcgagtg gaaatgcccg
    56461 gtacacaact acatcccgaa ctggctgaag ctcgccaacg aggggcgtat ttttgaagcg
    56521 gcggaactgt cgcatcagac caacactctg ccggaagtat gcggacgtgt ctgcccgcaa
    56581 gaccgtctgt gcgaaggttc ctgcaccctg aacgatgagt tcggcgcagt gaccatcggc
    56641 aacattgagc gctatatcaa cgataaagcg ttcgagatgg gctggcgtcc ggatatgtcc
    56701 ggcgtgaaac agaccggtaa aaaagtggcg attatcggcg caggcccggc gggtctggcg
    56761 tgcgcggatg tcctgacgcg caacggcgta aaagcggtcg tcttcgaccg tcacccggaa
    56821 attggcggat tgctgacctt cggtattccg gctttcaagc tggaaaaaga ggtaatgacg
    56881 cgccgccgtg aaatcttcac cggcatgggt attgaattca aactcaatac cgaagtgggc
    56941 cgcgacgtac agctggacga tctgctgagt gattacgatg ccgtgttcct tggcgtcggg
    57001 acttatcagt caatgcgcgg cgggctggaa aacgaagacg ccgatggcgt atacgcagcg
    57061 ctgccgttcc ttatcgccaa caccaaacag ttaatgggct ttggcgaaac ccgcgaagaa
    57121 ccgttcgtca gcatggaagg caaacgcgtg gtggtacttg gcggtggcga cactgcgatg
    57181 gactgcgtgc gtacgtctgt acgccaggga gcgaagcacg ttacctgtgc ctatcgtcgt
    57241 gatgaagaga acatgccagg ttcccgccgc gaagtgaaaa acgcgcgaga agaaggtgta
    57301 gagttcaaat tcaatgtcca gccactgggg attgaagtga acggtaacgg caaagtcagc
    57361 ggcgtaaaaa tggtacgtac cgaaatgggc gaaccggatg ccaaaggccg tcgccgcgcg
    57421 gagatcgtgg caggttccga acatatcgtt ccggcagatg cggtgatcat ggcgtttggt
    57481 ttccgtccac acaacatgga atggctggca aaacacagcg tcgagctgga ttcacaaggc
    57541 cgcatcatcg ccccggaagg cagcgacaac gccttccaga ccagcaaccc gaaaatcttt
    57601 gctggcggcg atatcgtccg tggttccgat ctggtcgtta ccgccatcgc cgaaggtcgt
    57661 aaggcggcag acggcattat gaactggctg gaagtttaag ctctgtccca gcgccgcctc
    57721 ctcagaaaag agtcggcgct gaaattctcc tttctttctg gaaagcgcct cgcataatct
    57781 gaagaaaacc aatgtaatac gctcatctgg attgttaccg gcagatgccc ggcgtatagc
    57841 tgattccatt attcaacaca gcgccataaa aagggacaga taatggaatc gctctcagag
    57901 agaaccacag caggctacca gcaaatccac gacggcatta ttcatctggt cgatagcgcc
    57961 cgaacggaaa cggtacgtag cgttaacgcg ttaatgaccg cgacgtactg ggaaattggc
    58021 cgacgaattg tcgaatttga acaaggcggt gaggccaggg ctgcgtatgg tgcgcagcta
    58081 atcaagcgac tatcaaagga tttaagtcta aggtataagc gtgggttctc tgcaaaaaac
    58141 ttacgccaaa tgaggctttt ttacctcttt tttcaacatg ttgaaattcg ccagacagtg
    58201 tctggcgaat taacaccatt gggaatgccg cagacaccgt ctgcagaatt cccatcggca
    58261 aagatttggc agacgctgtc tgccaaatct ttccccctgc cctggtccac ctacgtccgt
    58321 ctactttccg ttaaaaacgc tgacgcccgc agcttttatg aaaaagagac gctccgctgt
    58381 ggttggtctg tccgccagct agagcggcaa attgcgaccc aattttatga gcgaacacta
    58441 ctgtcacatg ataaatcagc catgctgcaa caacacgctc ctgccgagat gcatattctt
    58501 ccgcagcagg cgatacgcga tccctttgtg ctcgaatttc tggaattgaa agatgaatac
    58561 tcagaatccg attttgagga ggcgctgatc aaccacctga tggatttcat gctggaactt
    58621 ggggatgatt ttgcctttgt tggtcggcag cgaaggttac gcattgatga caactggttt
    58681 cgggtcgatc tgctgttttt ccaccgccgt ttacgctgcc tgctaatcgt cgatctaaaa
    58741 gtgggcaaat ttagctatag cgatgccgga cagatgaata tgtatctcaa ctacgccaaa
    58801 gagcactgga cgctaccgga tgaaaatccg cccatcggtc tgattctctg tgcagagaaa
    58861 ggagtcggag aagcgcatta tgcactggct ggtttgccta acaccgttct ggcgagtgaa
    58921 tataagatgc aactacctga tgaaaaacga ctcgcggatg aactcgttcg aacacaggcg
    58981 gtgctagagg aaggctatag acgccgataa tttcagcaat ttcgaattct gcagacagtg
    59041 tctgcagaat ctttaaaacg aactaagttt taagccatta aatcagcctt aaccttcact
    59101 accacctttt taatctcact aggttcgccg acaacgcatc ccggcttatg cggttctcca
    59161 ggcataaaca cggcgaacat tcccggcttc aggatgatgg tttgctcatt ctcaatggcg
    59221 ctgcaaagct ggtaatcatc ttcatggtgg aacacttcac actgacgcgc agtgcctgcc
    59281 atgccaaaca ggatccgttc ctcaccgttt aataacagct ggatatcaat gtactgctcg
    59341 tgcaattccg cttttttctc gacgggcgat tgagtgttaa acgtcatgac attcataaaa
    59401 atattgtcgc cctgtaattc gtaacgaccc ggcgcttttt cttgcggtct ggcagctaat
    59461 gccagcgtta acgcgtcctg taacgcagga tgtaacccag cagacggtaa tgactgtact
    59521 tcacccatca tcataatttt tctccctggg ccaacagcgc agccccaagt aaacctgcat
    59581 catggcggta atgcgccgcc agtaaatcaa catgaaatgc cgctggctcc tgcgccagat
    59641 acgtttccac cagcgccaga tacccttctg ccagcccaac gctgccaccg accaccacgc
    59701 actggcaatc agttgtggct ttaatatcag cgatcagcct tgcaagcgta cgtgcggagc
    59761 gatgaatcag ccattgcgct tgttcgtcgc cctgcccggc gcgtgtaaaa atagttctcg
    59821 catccgcgcc cgccagttca ccctgcgctg ccgctgcaat accgcgacca gaagcaatcg
    59881 cttccacgca acctgtgcgt ccacagccgc agactggacc atgcggatcg gcaagcgtat
    59941 gcccgatgtg ccccgccaga cctccagggc cggtaagcag tttgccgccg ctcactacac
    60001 cgccgccgac gccagtggaa acggtgataa acaccatctc ggttatatcg ccttccagcg
    60061 cctgatactc cgcccatgct gcggcctgcg cgtcgttaat ggcaatggtc ggcaaatcgg
    60121 taagctgccc cagtgtttta actaacggaa agtgtagtaa tccaccgaga ttatgcggat
    60181 ttagcgccat taagctgccg tcgcgaataa ttccggttga agcgatagca acccgctgcg
    60241 catgaacttg caacggagag actaatgcgg ataaggcatc acgcaaggct tctggtgtct
    60301 ggctggctgg cgtaggaagt tcacgacgat cgcggatctg cccgtcagcg ccaatcagcg
    60361 cggcggcaag tttagtaccg ccgatatcaa tcgccagtgt ggtcatagca ccgccttttt
    60421 catcgctgtg ttgtaccact gacaaatgtg ttcaagacgc gtaatggcag aaccgaccgt
    60481 caccgcccac gcgccgtggc gcatcgcatc tgccgcctga gcaggcgtgt tgtaacgccc
    60541 ttcggcaatc acccgacatc cggcgtcgct caacgttttc accagcgcca gatccggctc
    60601 ttctggcgtt tcaggcgtgg tatagccaga aagcgtagtg ccaataattt cggctcccag
    60661 cttttggcat gccaggccgt cttccggcgt tgagcagtcg gtcatcgcca gtaaaccgtg
    60721 atggtgaata cgtgccagca acgtttcaac aggcaccgga cgcgggcggt cggtgccgtc
    60781 aatggcgata atgtccgcgc ccgcctgcgc cagcgcatca acatcttcaa tataggccgt
    60841 gatgcgtacc ggagaatcct ccagatcgcg tttcacaatc ccgataattg gcacgctcac
    60901 caccgcacgc gtggcttgca gatttgccac accttcaatg cgaatggcaa ccgcgcccgc
    60961 ctgctctgcc gctaatgcca tggcggcgac gatttcgggt ttatcgagcg ggctgtccgg
    61021 aaccggctgg caggagacaa tcaggccacc gttagcagcg attttttgat ccagttgtgc
    61081 aagtaacgac atacatcttc ccttagcgaa aggcccggta cagagaccgg gcaacaggat
    61141 taacttttgg ttttgactaa atcgtttttg gcgcggccaa atggcacggc accgctaaat
    61201 ggtttgccgt cgatagcgtc atgagtacgc aatgcttccg ggcgcaacca acgctgaacg
    61261 cgggaaggca tatccagacc aatcagcagg atcaccacaa atgtcagact gaacgagagc
    61321 gatgccagcg cagtacccag atccagacgt tgagcgatca acgcgccgat gattggggcc
    61381 agtgcaccgc ccaatgcgcc gacgttgtag gtaaagccca ggcccgctgc acgctggtcg
    61441 gtatcgaaat aaccgccaat cagtttcggt aagatcccgg cgatcccttg tccaagcatt
    61501 tgctggaaga acagtaacag accgagcacc cagacgtttg cgccgccaat cgcaaatacc
    61561 ggaataatca gcagctgcga ggccagcagg ctacaaacgt acgctttgcg ggttcccagc
    61621 cagtcaccga ggaagccacc tacgcagcat cccaccgccg cgccaaagcc actaaagaac
    61681 agcacattgg cgacagtatg cgggttataa gccagatcgg ttttcagata cgttggcagc
    61741 agcgcctgaa tcggccatga gtagaggaaa gcaaacaaca cgaccaccat cagcattacg
    61801 cccgttggcc agcgtttgcc cgtactttgc accataaagc tgataaagat tgcggcgcat
    61861 aacagcccaa gaacagcgac gatcgcggca ttttgcaggt taccggcgaa gcaaaaccac
    61921 agcgcagtag ccgccgccag tgtcattacg atattggcaa tgcgatgttc accacggtag
    61981 agaatatcta ccatcgtgcg tactggtgct ttaccagcat gtttctcttt ccagtcttcc
    62041 gcttccggga tgtttttacg cagccagaga gcaaagatga ttggcaaaat gccgataaag
    62101 aacagcgcac gccagcccca gaccggaacc accaggctat agacctgagc ggcaacgacg
    62161 gcccccacag agaagccgga aatcagaaaa ccactggctt tattacgcag atgttttggc
    62221 cagctttcaa tgacataggt ggcgctggaa ccgtattcac ccgccatccc catgccgatg
    62281 accagacgag cgataaacat ggtgatgtag cctggcgcaa agccgcaggc cagcgtcccg
    62341 gccgagaaga gaacgatgct ggtgaccatt gccagacgac gcccgtagcg gtcacccata
    62401 gcgccgagca tcaggccgcc gaaccagcga gagataaagg ctgcggagat cagacttgcc
    62461 gcctgcaccg tcgtcagccc gaattcacct tgtacttcgg tgagtaccag ggcgattaaa
    62521 acgaaatcaa aaccgtcaag cagatatccc aaccaggcag cggaaaatgc ccgccattgt
    62581 gcacgattga gatggcgata ccacgggata ttctgggttg tagtactcat tgtgagtctc
    62641 ccgcggtggg cgatgcccac acgctttggt atgaaaattg tagggtacag atgcgtttat
    62701 ttcccctcac ccggtagggg cgagcgaggg gaaacaactc acccgcgctc ttgcatcaac
    62761 tgctgggcca gcgccttcag ttctggcaga tatttttcat ctaccggtcc aaacggtttg
    62821 cggcacagcg gcacagaaac gacatccata taatgcagga ccgttttcag gccgcggaat
    62881 acgcccgttt tgatcagtaa atcaatgact ttattacatt cggtttgcag cttctgcgcg
    62941 gtctggatat cgccttcttt cagcgccttc acaatcccct gataacgcca gcccatgatg
    63001 ttgtaggtac taccgatacc accatcagcg cccgccagaa gaccagaggc gaagatttcg
    63061 tcgtaaccgt tatagagcac cagatcagga tgttcacgac ggatctgttc catctgatag
    63121 agatcgccag aggtctgttt cagcgcgccg acaccaggca gcgtaaccag ggtgttgatc
    63181 tgatccaggg ttagttttac cccgctcagg gcaggaatgt tgtacaccac catcggcaaa
    63241 ccatccgccg aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga
    63301 tagtagaacg gcgtgacggc ggagacggca tcgaagccat aacgtttagc cgatgccgca
    63361 agttgttggc tttcagcagt gctgacgcaa ccgacgtgag cgatgagttt aatcttgcct
    63421 ttcgcctctt cggcgacgat ttccagtacc tgttcacgct cggaaaggct ttgtacaaag
    63481 gcttcgccgg tagaaccacc cacgtataaa ccgtcgatgc cctgctgaat attgaactga
    63541 accaggcgac gcagactcgc tttatccagt gcttgttgtt gatcaaaagg agtcaggagt
    63601 gcggccatta cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga
    63661 taaaacgata tacctttata cctgttatac cagatcaatt aagcaacacc ccatacagaa
    63721 agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg gtgctctgac
    63781 cgaaggcatg ccaggtagca gagacgctgt tgagatgcga ttgcaacgcg cgatcggctt
    63841 catcgggatc atggcggcgg atcgcgtcaa cgatcgcaat atgctgttga tagctaacgt
    63901 tgttatgctc gtgcagagcc tgatcagcca cggttggacg tgcggcgatc agccagtcga
    63961 gcaaggcaac gtggatcgcc atgaagattg ggttaccggg gatctccgcc agcacgcggt
    64021 ggaaatcaac gtctgaacga atgaatgccg cgttgttatc cagcgactga ctgttgattt
    64081 ccagtgcttt tgccagcaaa tcgatttgct catcggtggc gtgttcagcc gcatagcgca
    64141 ccaggctgga ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca ccaggatgag
    64201 aaaggaaatc tttcgccatg ccggaaagct cgccgatgat ggtgtccgca gaaggacgtg
    64261 agacgcgagc gcgttcgccg ttgtttattt gcaccagacc tttacgtttt aacgctgcca
    64321 gcgcttcacg caccgaagga cgcccgacat taaagaacgc catcagttcg cgttcagacg
    64381 gtaattgttc accttcgcca aattcacgac ggcggatcat ctgttccagc tcttcttcca
    64441 ccatttcgga gagtttttta cgcgccagcg ggcggctacg caagttgcga ccaattgcag
    64501 gtgaagaatc ttcggtttgc gaatcaaatg cgttcataag gcccattctg taaggtcagt
    64561 gtgattaaca tcatcagtga catcctatca caggattgaa agtaggggaa aatggcaggg
    64621 ttttctcttt gtgcctcatc attaccataa ttaacggaat aattaactat tgcgaaaaat
    64681 taatgtaacg cagataaaaa catcccgatt gaattattta taaga
//
LOCUS       DAYPHN010000009        54787 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000009 DAYPHN010000000
VERSION     DAYPHN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.009"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(173..940)
                     /gene="ssuB"
                     /locus_tag="ACG0BW_000577"
     CDS             complement(173..940)
                     /gene="ssuB"
                     /locus_tag="ACG0BW_000577"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFK7257589.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGSTPLAQIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLQGQWRDAARRALAAVGLENRAREWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(937..1728)
                     /gene="ssuC"
                     /locus_tag="ACG0BW_000578"
     CDS             complement(937..1728)
                     /gene="ssuC"
                     /locus_tag="ACG0BW_000578"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFK7257590.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(1740..2885)
                     /gene="ssuD"
                     /locus_tag="ACG0BW_000579"
     CDS             complement(1740..2885)
                     /gene="ssuD"
                     /locus_tag="ACG0BW_000579"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFK7257591.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHRERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDFYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETSDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANEFIPRKVAQS"
     gene            complement(2882..3841)
                     /gene="ssuA"
                     /locus_tag="ACG0BW_000580"
     CDS             complement(2882..3841)
                     /gene="ssuA"
                     /locus_tag="ACG0BW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFK7257592.1"
                     /translation="MRNIIQLALAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVPENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPTPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLMPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(3834..4409)
                     /gene="ssuE"
                     /locus_tag="ACG0BW_000581"
     CDS             complement(3834..4409)
                     /gene="ssuE"
                     /locus_tag="ACG0BW_000581"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFK7257593.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDIQVPDLLSLRGNAHA"
     gene            4646..5656
                     /gene="pyrD"
                     /locus_tag="ACG0BW_000582"
     CDS             4646..5656
                     /gene="pyrD"
                     /locus_tag="ACG0BW_000582"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFK7257594.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            5830..6372
                     /gene="zapC"
                     /locus_tag="ACG0BW_000583"
     CDS             5830..6372
                     /gene="zapC"
                     /locus_tag="ACG0BW_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFK7257595.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(6369..7478)
                     /gene="ycbX"
                     /locus_tag="ACG0BW_000584"
     CDS             complement(6369..7478)
                     /gene="ycbX"
                     /locus_tag="ACG0BW_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFK7257596.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            7722..9830
                     /gene="rlmKL"
                     /locus_tag="ACG0BW_000585"
     CDS             7722..9830
                     /gene="rlmKL"
                     /locus_tag="ACG0BW_000585"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFK7257597.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWAHQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            9842..11749
                     /gene="uup"
                     /locus_tag="ACG0BW_000586"
     CDS             9842..11749
                     /gene="uup"
                     /locus_tag="ACG0BW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7257598.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLKDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            11879..13132
                     /gene="pqiA"
                     /locus_tag="ACG0BW_000587"
     CDS             11879..13132
                     /gene="pqiA"
                     /locus_tag="ACG0BW_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFK7257599.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPALRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13137..14777
                     /gene="pqiB"
                     /locus_tag="ACG0BW_000588"
     CDS             13137..14777
                     /gene="pqiB"
                     /locus_tag="ACG0BW_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFK7257600.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            14774..15337
                     /gene="pqiC"
                     /locus_tag="ACG0BW_000589"
     CDS             14774..15337
                     /gene="pqiC"
                     /locus_tag="ACG0BW_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFK7257601.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            15593..15760
                     /gene="rmf"
                     /locus_tag="ACG0BW_000590"
     CDS             15593..15760
                     /gene="rmf"
                     /locus_tag="ACG0BW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFK7257602.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(15830..16348)
                     /gene="fabA"
                     /locus_tag="ACG0BW_000591"
     CDS             complement(15830..16348)
                     /gene="fabA"
                     /locus_tag="ACG0BW_000591"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFK7257603.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(16417..18177)
                     /gene="ycbZ"
                     /locus_tag="ACG0BW_000592"
     CDS             complement(16417..18177)
                     /gene="ycbZ"
                     /locus_tag="ACG0BW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFK7257604.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NTVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRGRFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18363..18815
                     /gene="matP"
                     /locus_tag="ACG0BW_000593"
     CDS             18363..18815
                     /gene="matP"
                     /locus_tag="ACG0BW_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFK7257605.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(18891..19931)
                     /gene="ompA"
                     /locus_tag="ACG0BW_000594"
     CDS             complement(18891..19931)
                     /gene="ompA"
                     /locus_tag="ACG0BW_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFK7257606.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(20287..20796)
                     /gene="sulA"
                     /locus_tag="ACG0BW_000595"
     CDS             complement(20287..20796)
                     /gene="sulA"
                     /locus_tag="ACG0BW_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFK7257607.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            21015..21644
                     /gene="sxy"
                     /locus_tag="ACG0BW_000596"
     CDS             21015..21644
                     /gene="sxy"
                     /locus_tag="ACG0BW_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFK7257608.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(21607..23769)
                     /gene="yccS"
                     /locus_tag="ACG0BW_000597"
     CDS             complement(21607..23769)
                     /gene="yccS"
                     /locus_tag="ACG0BW_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFK7257609.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(23779..24225)
                     /gene="yccF"
                     /locus_tag="ACG0BW_000598"
     CDS             complement(23779..24225)
                     /gene="yccF"
                     /locus_tag="ACG0BW_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFK7257610.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            24348..26402
                     /gene="helD"
                     /locus_tag="ACG0BW_000599"
     CDS             24348..26402
                     /gene="helD"
                     /locus_tag="ACG0BW_000599"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFK7257611.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LLQQLDLIATRTGENKWLTREQTSGIQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(26434..26892)
                     /gene="mgsA"
                     /locus_tag="ACG0BW_000600"
     CDS             complement(26434..26892)
                     /gene="mgsA"
                     /locus_tag="ACG0BW_000600"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFK7257612.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATSMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(26988..27650)
                     /gene="yccT"
                     /locus_tag="ACG0BW_000601"
     CDS             complement(26988..27650)
                     /gene="yccT"
                     /locus_tag="ACG0BW_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFK7257613.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            27823..28236
                     /gene="yccU"
                     /locus_tag="ACG0BW_000602"
     CDS             27823..28236
                     /gene="yccU"
                     /locus_tag="ACG0BW_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFK7257614.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(28281..28598)
                     /gene="hspQ"
                     /locus_tag="ACG0BW_000603"
     CDS             complement(28281..28598)
                     /gene="hspQ"
                     /locus_tag="ACG0BW_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFK7257615.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(28656..29846)
                     /gene="rlmI"
                     /locus_tag="ACG0BW_000604"
     CDS             complement(28656..29846)
                     /gene="rlmI"
                     /locus_tag="ACG0BW_000604"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFK7257616.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWIFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLMRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            29941..30219
                     /gene="yccX"
                     /locus_tag="ACG0BW_000605"
     CDS             29941..30219
                     /gene="yccX"
                     /locus_tag="ACG0BW_000605"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFK7257617.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(30216..30545)
                     /gene="tusE"
                     /locus_tag="ACG0BW_000606"
     CDS             complement(30216..30545)
                     /gene="tusE"
                     /locus_tag="ACG0BW_000606"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFK7257618.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(30636..31295)
                     /gene="yccA"
                     /locus_tag="ACG0BW_000607"
     CDS             complement(30636..31295)
                     /gene="yccA"
                     /locus_tag="ACG0BW_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFK7257619.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(31502..31589)
                     /locus_tag="ACG0BW_000608"
     tRNA            complement(31502..31589)
                     /locus_tag="ACG0BW_000608"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31553..31555),aa:Ser,seq:tga)
     gene            32016..33134
                     /gene="hyaA"
                     /locus_tag="ACG0BW_000609"
     CDS             32016..33134
                     /gene="hyaA"
                     /locus_tag="ACG0BW_000609"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFK7257620.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            33131..34924
                     /gene="hyaB"
                     /locus_tag="ACG0BW_000610"
     CDS             33131..34924
                     /gene="hyaB"
                     /locus_tag="ACG0BW_000610"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFK7257621.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            34943..35650
                     /gene="hyaC"
                     /locus_tag="ACG0BW_000611"
     CDS             34943..35650
                     /gene="hyaC"
                     /locus_tag="ACG0BW_000611"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFK7257622.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            35647..36234
                     /gene="hyaD"
                     /locus_tag="ACG0BW_000612"
     CDS             35647..36234
                     /gene="hyaD"
                     /locus_tag="ACG0BW_000612"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFK7257623.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARERLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGIRLRQYRMTLEEQR"
     gene            36231..36629
                     /gene="hyaE"
                     /locus_tag="ACG0BW_000613"
     CDS             36231..36629
                     /gene="hyaE"
                     /locus_tag="ACG0BW_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFK7257624.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            36626..37483
                     /gene="hyaF"
                     /locus_tag="ACG0BW_000614"
     CDS             36626..37483
                     /gene="hyaF"
                     /locus_tag="ACG0BW_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFK7257625.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRCGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRFFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            37617..39161
                     /gene="appC"
                     /locus_tag="ACG0BW_000615"
     CDS             37617..39161
                     /gene="appC"
                     /locus_tag="ACG0BW_000615"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFK7257626.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALSSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            39173..40309
                     /gene="appB"
                     /locus_tag="ACG0BW_000616"
     CDS             39173..40309
                     /gene="appB"
                     /locus_tag="ACG0BW_000616"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFK7257627.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGAIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            40322..40414
                     /gene="cbdX"
                     /locus_tag="ACG0BW_000617"
     CDS             40322..40414
                     /gene="cbdX"
                     /locus_tag="ACG0BW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFK7257628.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            40494..41804
                     /gene="appA"
                     /locus_tag="ACG0BW_000618"
     CDS             40494..41804
                     /gene="appA"
                     /locus_tag="ACG0BW_000618"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFK7257629.1"
                     /translation="MKAILIPFLSLLIPFTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDKSCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELLFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(41792..42004)
                     /gene="cspH"
                     /locus_tag="ACG0BW_000619"
     CDS             complement(41792..42004)
                     /gene="cspH"
                     /locus_tag="ACG0BW_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFK7257630.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            42290..42502
                     /gene="cspG"
                     /locus_tag="ACG0BW_000620"
     CDS             42290..42502
                     /gene="cspG"
                     /locus_tag="ACG0BW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFK7257631.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            42513..42701
                     /gene="ymcF"
                     /locus_tag="ACG0BW_000621"
     CDS             42513..42701
                     /gene="ymcF"
                     /locus_tag="ACG0BW_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFK7257632.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            42676..42906
                     /gene="ymcE"
                     /locus_tag="ACG0BW_000622"
     CDS             42676..42906
                     /gene="ymcE"
                     /locus_tag="ACG0BW_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFK7257633.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            42896..43069
                     /gene="gnsA"
                     /locus_tag="ACG0BW_000623"
     CDS             42896..43069
                     /gene="gnsA"
                     /locus_tag="ACG0BW_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFK7257634.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(43118..44191)
                     /gene="yccM"
                     /locus_tag="ACG0BW_000624"
     CDS             complement(43118..44191)
                     /gene="yccM"
                     /locus_tag="ACG0BW_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFK7257635.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIVSLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(44263..47006)
                     /gene="torS"
                     /locus_tag="ACG0BW_000625"
                     /pseudo
     CDS             complement(44263..47006)
                     /gene="torS"
                     /locus_tag="ACG0BW_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            47089..48117
                     /gene="torT"
                     /locus_tag="ACG0BW_000626"
     CDS             47089..48117
                     /gene="torT"
                     /locus_tag="ACG0BW_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFK7257636.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIHRSLSPGGFRPVYYY
                     QHTSAAKK"
     gene            complement(48090..48782)
                     /gene="torR"
                     /locus_tag="ACG0BW_000627"
     CDS             complement(48090..48782)
                     /gene="torR"
                     /locus_tag="ACG0BW_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFK7257637.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            48912..50084
                     /gene="torC"
                     /locus_tag="ACG0BW_000628"
     CDS             48912..50084
                     /gene="torC"
                     /locus_tag="ACG0BW_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFK7257638.1"
                     /translation="MRKFWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            50084..52630
                     /gene="torA"
                     /locus_tag="ACG0BW_000629"
     CDS             50084..52630
                     /gene="torA"
                     /locus_tag="ACG0BW_000629"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFK7257639.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRSATAAQAAT
                     EAVTSKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            52627..53226
                     /gene="torD"
                     /locus_tag="ACG0BW_000630"
     CDS             52627..53226
                     /gene="torD"
                     /locus_tag="ACG0BW_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFK7257640.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(53319..53624)
                     /gene="cbpM"
                     /locus_tag="ACG0BW_000631"
     CDS             complement(53319..53624)
                     /gene="cbpM"
                     /locus_tag="ACG0BW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFK7257641.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(53624..54544)
                     /gene="cbpA"
                     /locus_tag="ACG0BW_000632"
     CDS             complement(53624..54544)
                     /gene="cbpA"
                     /locus_tag="ACG0BW_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFK7257642.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        13376 a        13847 c        14581 g        12983 t
ORIGIN      
        1 gacgcgtctt atcaggccta caagtccgtt gaaaatgctg gttttgtagg ctggataagg
       61 cgttcacgcc gcatccggca gccgtgccca aatgcctgat gcgccgcttg acgcgtctta
      121 tcaggcctac aatccgttga aactgctggt tttgtgcgcc acatccggca tattaaccat
      181 gacgccgaat cggttgttca gagtgcccac gctgcatcac ccgttgcaac acttccgctt
      241 ccagctccgc taatcgcacc gaccctaacc ggcgcggacg ggggatatcc accgtcaaat
      301 ccagaccaat ttttccctct tcaattaata acacccggtc cgccattgct accgcttcgc
      361 tcacatcatg cgtcaccaac agtacggtaa agccgtgctc ctgccaaagt gacacaatca
      421 aatcctgcat ctcgagccga gtcagggcgt ccagcgcccc gagcggttca tcaagcaata
      481 ataaacctgg tcgatgaatc aacgctcttg ccagcgccac acgctgtttc tgcccgccag
      541 aaagtgcggc aggccattcc cgcgcgcgat tctccagccc taccgcagcc agcgcccgac
      601 gcgcggcatc gcgccactga ccttgaaggc ctagcccaac gttatcaatc accgatttcc
      661 acggcagcag acgcgcatcc tgaaacatca ttcgcgtgtc ttcctgaatc tgagccagcg
      721 gtgtggaacc cgctaacaca tcgccagcgg ttggcgtttc cagacctgcc agcaggcgca
      781 gcaaggtact tttgccacca ccgctgcggc ccaccaccgc cacaaactga cctgccggaa
      841 tatgtaaatc cagttggttc aggacgatat tttctgcgta atgtttgctt actgcattga
      901 gcaacaatgg tgtgccctgg ttcagacgag cagtattcat accgtggcct ccttcaaatg
      961 ataagccggg ttccagcgca accagaggcg ctctaacagc tgcgcgctga cgtcagcaag
     1021 tttgccgagc agggcgtaaa gaataatagc gacaaccacc acgtccgttt gcagaaactc
     1081 ccgcgcattc atcgccagat aaccaatgcc ggaattggca gaaatagttt cggcaacgat
     1141 cagtgtcagc cacatcaggc ccaacgcaaa acgcacgccg accataattg agggcagggc
     1201 gccaggcagg atcacatgga taaacagcgg tatgccggat aatccatagc tacgcgccat
     1261 ctccaccagc ccgcgatcga tattacggat gccatgccag gtgttgatat aaatggggaa
     1321 tagcgtgcca agcgccacca gaaagatttt tgcggattca tcaatgccaa accacaaaat
     1381 caccagcggg atcagcgcca gatgcggcac gttgcgcaac atctgaattg aggtatccag
     1441 cagccgttct ccccagcgcg acagcccgct aatcagtccc agaatcaagc ccagcgatcc
     1501 gccaattgaa aagccaatga gcgcccgcca ggagctgatc gccagatgct gccacaattc
     1561 gccgctggcg gagagcgtcc agaacgccat cactacccct tccggtgagg gcaaaatacg
     1621 cgtcgacagc cagccaactg aggaggccag ttgccacacc gccacgatgc ccaccggtaa
     1681 aaaccagggg gcaacgcgca ataaccactt tttcactggc gttgccatca ttttgttcct
     1741 tagctttgcg cgactttacg ggggataaat tcatttgcca ccgcttcgcc ttgcggattc
     1801 agcggctgcg gctggggaat ttccgggatg gcgacatcca gatgcgggaa cagcaactcg
     1861 ccaacccgat acgcttcttc cagatgcggg tagcccgaaa gcacaaaact gtcgatgcca
     1921 agtgcagcgt attcgttgat tcgcgcggcg accgtcggac catcgcccac cagcgccgtc
     1981 ccggcaccgc cgcggactaa gccaacgccc gcccataaat tggggctgat ctccagattg
     2041 tcgcgcttgc cgttatgtaa cgccgccatc cgctgttgtc ctacggaatc cgtccgggcg
     2101 aatgcggcct gtgctttggc gatagtctca tcatcaagat gcgagattaa ccgctcggcg
     2161 gcctgccacg cttcgtcgct ggtttcacga acaatcacat gcagacgaat accgaaacga
     2221 attttgcgtc catgcgcggc agctttcgcc cgcacttgtt cgattttctc tttaaccagt
     2281 tccggcggtt cgccccaggt aaggtagaaa tctacttgtt ctgccgccag ctcctgcgcg
     2341 acatctgacg atccgccaaa gtaaagcgga ggatacggct gttgaatcgg cgggaagagt
     2401 agtttggctc cgcgcacatg aatatgttta ccgttgaaat tgacggtttc ccccagcaat
     2461 aagcgccgcc agacctgggt aaattccgcc gaggcttcgt agcgctcgcg atgatcaagg
     2521 aacacgccgt cgcctgccag ctcttgtgga tcgctgcctg tgaccagatt aaacaacgca
     2581 cgaccattcg agagacggtc aagcgtggcg gcctggcggg cggcaacggt aggagaggtt
     2641 acgctgggac gcagggcgac aagaaacttc agccgttgcg tcaccgggat catcgacgcg
     2701 gcaaccagcc acgcatcttc gcaggagcgc cccgttggga tcagcacgcc ggtatagccc
     2761 agacgatccg ctgcttgcgc aatttgttgc agataaccgt gatcaaccgg gcgtgaacct
     2821 tcttccgttc ccagataatg cccgtcaccg tgggttggta aaaaccagaa catattcaga
     2881 ctcataattg ttttccttcc agttgagtgg gctgccagat acgctggcga atatcgactt
     2941 ttttcggcat cagacggttt tcataaaaca gatctgccgt ttgctgctgt aaggcggcaa
     3001 cctcggcgtt aaccggtttg atggtggtag gggggcgatg atctaaataa gaggcaatca
     3061 ccggcgttgg taagcccatc gtttttgcca gtaaagcgat gctttgctcg cgctggctgc
     3121 gggttaacgc atcggcctca ctaaaggttg ccagtacgcc ctgaataaaa gcgccgtttt
     3181 tttctgcata cggacgggca gccagataaa acgatccggt ttgattgaga tcggtaccgt
     3241 cttttagcac ccgcacgccg ccctgtaata atgcagcgga gtagtaggga tcccagatag
     3301 cccatgcgtc gacgttacct tgctggaacg cggcgcgggc atcggcaggc gtcaggtaag
     3361 tgggctggat atccgtgaat ttaagcccgg cctggcgtag tgcgcgtagt aaaagattgt
     3421 gcgagctgga acctttctga aaggcaactt tgtgaccttt aagatcggct acggttttga
     3481 tcgggctgtt ttctggcacc agaatcactt cggctttggg cttcggcggc tcgacgccca
     3541 cgtacaccaa atcagccccg gcagcctggg caaagattgg cggaatatct ccggtactgc
     3601 cgagatcaat actgccaacg tttaacgcct ccagcatctg tgggccagcg gggaactcca
     3661 cccaggagat tttggtttgc ggatagcgtt tttccagtaa ctggtggctt tttgccagca
     3721 ccataccaat actgcctttc tgatagccta tacgtaacgc ttcaggagag gattctgcag
     3781 caaccgcaat cgtagagacg ctaagtaatc ccgccagcgc cagttgaatg atgttacgca
     3841 tgggcattac ctcgcagaga cagaagatcg ggaacctgaa tatcgcggcg gtgcagcgcc
     3901 tgccagaaag tttccagcgc ggtatcaaga cgggtttgca gatttggcgt gaactgcggc
     3961 ttgtggtggt aatcaatcac ctgtgagtca tcggcaaaca cgccgtgcag gatctcctgg
     4021 gctttcagcg cacttaaaac tggcttaagg gcataatcga ccgccagcag atgggccacc
     4081 gtaccgcccg tcgccagcgg tagcaccact ttgccttgca acgcacgttc tggcagtagg
     4141 tcgagcaggg ttttcagcgc accggaatag gcggctttat acacaggcgt ggcgacaatc
     4201 agcccatcgg cctgttgcag ctgttcggtg aaggttttga gtgccggact atcgaaacga
     4261 gcataaagta gatcttccgg ggcgaagttt tgcagattcc agtgataaac ctctacatcc
     4321 aggccattta gtttttcccg cgcatattcc agcaaggagc tggaacgaga aggaaagcga
     4381 ggactacccg ccagggtgat gacacgcata ctctctcctt ataaccaatt gttcttgttt
     4441 tgttaacatt gataacaatt cggtcagtct gacagagggc gtccgcgagt cgaaatgatt
     4501 tatctgagga agaaaagcca aaaaaagcat aaaaaaagcg caataagaaa acgtttgcta
     4561 tttattgcca caggtcaatt cccttttggt ccgaactcgc acataatacg cccccggttt
     4621 gcacaccggg aatccaggag agttcatgta ctaccccttc gttcgtaaag cccttttcca
     4681 gctcgatcca gagcgcgctc atgagtttac ttttcagcaa ttacgccgta ttacaggaac
     4741 gccgtttgaa gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact gcatgggcct
     4801 gacgtttaaa aatccgcttg gtctggcagc cggtcttgat aaagacgggg agtgcattga
     4861 cgcgttaggc gcgatgggat ttggatcgat cgagatcggt accgtcacgc cacgtccaca
     4921 gccaggtaat gacaagccgc gtctctttcg tctggtagat gccgaaggtt tgatcaaccg
     4981 tatgggcttt aataatcttg gcgttgataa cctcgtagag aacgtaaaaa aagcccatta
     5041 tgatggcgtc ctgggtatta acatcggcaa aaataaagat acgccggtgg agcagggtaa
     5101 agatgactat ctgatttgta tggaaaaaat ctatgcttat gcgggatata tcgccatcaa
     5161 tatttcatcg ccgaatacac cgggattacg tactctgcaa tatggcgaag cgctggatga
     5221 tctcttaacc gcgattaaaa ataagcaaaa tgatttgcaa gcgatgcacc ataaatatgt
     5281 gccgatcgca gtgaagatcg cgccggatct ttctgaagaa gaattgatcc aggttgccga
     5341 tagtttagtt cgccataata ttgatggcgt tattgcaacc aataccacgc tcgatcgttc
     5401 tctggttcag ggaatgaaaa attgtgatca aaccggtggc ttaagtggtc gtccgcttca
     5461 gttaaaaagc accgaaatta ttcgccgttt atcacaggaa ttaaatggtc gcttaccgat
     5521 catcggtgtt ggcggcatcg actcggttat cgctgcgcgt gaaaagattg ctgcgggggc
     5581 ctcactggtg caaatttatt ctggttttat ttttaaaggt ccgccgctga ttaaagaaat
     5641 cgttacccat atctaattat ttcttcgaat tcctctttat aagataatca gggctttatt
     5701 ttaagccctg gttgttttat attcatcgct gttgcttatt tagacatgtt gtacttttat
     5761 tgttgaggtt attaagcgaa gcgacaatgg attgtgttgc tgcggtttta tagggtaggg
     5821 gagaggcaaa tgcgaattaa accagatgat aactggcgtt ggtattacga tgaagagcac
     5881 gatcgaatga tgctcgattt agccaatggt atgctatttc gctcacgttt tgcgcgcaaa
     5941 atgttgactc cagatgcctt ttctcccgca ggcttttgcg ttgacgacgc cgcgctctat
     6001 ttctcatttg aagaaaaatg ccgcgatttt aatttatcta aagaacaaaa agccgaactg
     6061 gtgcttaatg cgctggtagc aattcgttat ctcaaaccgc aaatgccgaa aagctggcat
     6121 tttgtttccc atggtgaaat gtgggtgccc atgccgggcg atgcggcctg cgtttggttg
     6181 agcgacactc acgagcaagt caatttgctg gtcgttgaat ctggcgaaaa tgccgcacta
     6241 tgcctgctgg cacaaccctg cgttgttatt gcgggtcgcg cgatgcagct gggcgacgcc
     6301 atcaaaatca tgaacgacag gctgaaaccg caggttaatg ttgacagctt cagcctcgaa
     6361 caggcagtct aacgcgtcag cttaagcgca gtcttcggta cacagctaca gcaaagaatg
     6421 gtgccatcat cgcccattgc tgattttttc agcggcgtga cttcgccttc taaaagctga
     6481 acacggcaac ttccacaaat gcccgcgcgg caagaataag ggatacgaat tccctgattt
     6541 tctaattgct ccagcaacac ctgctggtta tttccacgaa atgcctgtcc ctgccagtca
     6601 atatctacat ttgcgtccgg ttgttgcgtg atgttgacgg tatcatcagc ggcggctgcg
     6661 ccgtaaattt tagccggagc cgttgccaga atttccacct catcgccaac gcgaattacg
     6721 ccgctattac gggcaattaa attctggcca aaatcgacat cgccgttatc ctgggcagtg
     6781 cggaaagatt gcaatgtttt taatggttcg cctgccggat gtttttgccc tttttctggg
     6841 ctgacggtgg tgaaaataca gcggctacaa ggtttaacca catcaaacac cacatcacca
     6901 atacgaatca ctttccaact atcttcttcc caggctgacg cgccggaaac caccagattg
     6961 gggcggaatt gctccatttt tacactggcc ggacaacgtt gttggagatc acgtaacgag
     7021 gcttcgttag caagaaggta aggatagcca tcagcaaatg acaggggtac agtgttgtgg
     7081 cgtttcacgc gccgcgtcat ttgtggcccc acccagcgca actgcacttc gcgggagaaa
     7141 aatccactta gccatttgtt gatcgcgtct ggcgcaattc gcgcggtaaa atgtgtgccc
     7201 caaacttcgg ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg
     7261 ccatctggtg cggtgagatg caagccatca tgcacgggtg aaggagtaaa ccgtaccatt
     7321 tgcggaaact ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat aaagatgcga
     7381 tcgaaggcca gaccactgac atctgccagg gcatgtgtaa gaccaatgcc gcgcatcgat
     7441 ttaacaggat gaataaaaag ccgggttaat gtcgccacgg tgcggtcctc aaatgaaaat
     7501 aagccctcaa ctttatgaca tgacgcgctt attagctata atgcgcaaca attttcttag
     7561 cgcctgtccc attaggctat tttatttgtc agtttggccc tgggttgtgc tcgaaatcct
     7621 cacgtacttg tgtacgctcc ggtttctccg cgctgtccat gtccagactg actgcaacaa
     7681 ttacgcctac tgcggtaggc tcttagagta aaagtgacga tatgaattct ctgtttgcca
     7741 gtacggcccg tgggctggaa gagctgttaa aaactgaact ggaaaacctg ggggccgttg
     7801 aatgccaggt ggttcagggt ggggtccatt tcaagggcga cacacggctt gtttaccaga
     7861 gcctgatgtg gagccgcctg gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt
     7921 acagcgattt agacctctat ctcggtgttc aggcgatcaa ctggacagag atgtttaatc
     7981 ctggtgcgac cttcgctgtc cacttcagtg gcttgaatga caccatccgc aacagtcagt
     8041 acggtgcgat gaaagtgaaa gacgcgatcg tcgatgcttt cacgcggaaa aatctgccgc
     8101 gtccaaatgt tgatcgcgat gcgccggata tccgcgttaa cgtctggctg cataaagaaa
     8161 ctgccagtat cgcccttgat ctcagtggtg atggtttaca tctgcgtggc tatcgcgatc
     8221 gtgctggtat tgcgccgatc aaagaaaccc tggcagccgc gattgtgatg cgatccggct
     8281 ggcagccagg aacaccgctg ctcgatccaa tgtgtggttc cggtacgttg ctgattgaag
     8341 cagcgatgct ggcgaccgat cgcgcacctg gcttgcaccg tgggcgttgg ggctttagcg
     8401 gctgggcaca gcatgatgaa gctatctggc aggaagtgaa agcggaagcg caaactcgcg
     8461 cccgtaaagg cctggctgag tatagctctc atttttacgg ttcggacagc gacgcacggg
     8521 tgattcaacg tgcgcgcact aacgcccgtc ttgcggggat tggtgaactg atcacctttg
     8581 aggtgaaaga tgtcgcgcaa ctgaccaatc cgctgccgaa agggccgtac ggtacagtgt
     8641 tgagcaaccc gccatacggt gaacgtctgg acagcgaacc ggcgctgatt gcgctacata
     8701 gtctgctggg ccgaatcatg aaaaaccagt tcggtggctg gaatctctct ttgtttagtg
     8761 cctcgccgga tctgctcagc tgcctgcagc tgcgtgcaga caaacagtac aaggcgaaaa
     8821 acggcccgct ggactgcgta cagaaaaatt accacgttgc cgaaagcact ccagacagca
     8881 aaccggcgat ggtagcggaa gactacgcta accgcctgcg taagaacctc aaaaaattcg
     8941 agaagtgggc tcaccaggaa gggattgaat gttaccgcct gtatgacgcc gatctgccgg
     9001 aatataacgt tgccgttgat cgttatgccg actgggtggt ggtgcaggag tatgcgccgc
     9061 caaaaactat tgatgcgcac aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca
     9121 tttcggtact ggggattgcg ccaaacaaac tggtgctgaa aacccgtgaa cgccagaagg
     9181 gcaaaaatca gtaccagaaa ctgggcgaga agggcgagtt tctcgaagtc accgaatata
     9241 acgctcactt gtgggtgaac ctgacggatt acctcgatac cggtctgttc ctcgatcacc
     9301 gtatcgcccg tcgtatgctc ggtcagatga gcaaaggcaa agatttcctc aacctgttct
     9361 cttataccgg cagcgccacc gtgcacgcgg gattaggcgg tgcacgcagc actaccaccg
     9421 tggatatgtc gcgtacttat ctggagtggg cagaacgcaa cctgcgtctg aatggcctaa
     9481 ccgggcgtgc gcatcgcctg attcaggccg attgcctggc gtggctgcgt gaggcaaatg
     9541 aacagttcga tctgatcttt atcgatccgc caaccttctc taactcaaaa cgaatggaag
     9601 atgcgtttga tgttcagcgc gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc
     9661 gtgctggtgg gacgatcatg ttctcaaaca acaaacgtgg cttccgtatg gatcttgacg
     9721 gactggcaaa actgggactg aaagcacaag aaattacgca aaaaacgctc tcccaggatt
     9781 tcgcccgtaa ccgccagatc cacaactgct ggctgattac cgcagcctga aaggaatagt
     9841 aatgtcatta atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga
     9901 taacgcagaa ctgcatatcg aagataacga acgtgtttgt ctggtaggcc gtaacggcgc
     9961 aggcaaatcg acgttaatga aaatcctcaa ccgtgaacag gggctggatg acggtcgcat
    10021 tatttacgag caagatttga ttgtcgctcg tctgcaacag gatccgccgc gtaacgttga
    10081 gggtagcgtt tatgatttcg ttgccgaagg cattgaagaa caagcggaat acctgaagcg
    10141 gtatcacgat atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact
    10201 ggcaaaagtt caggaacagt tggatcacca caacctgtgg cagctggaaa accgcatcaa
    10261 cgaagtgctg gcgcaactgg ggttagatcc taacgttgcg ctgtcgtcgc tttccggcgg
    10321 ctggttgcgt aaagcggcat taggacgcgc gctggtgagc aatccgcgcg tattgctgct
    10381 cgacgaaccg actaaccacc tggatatcga aaccatcgac tggctggaag gattccttaa
    10441 aaccttcaat gggacgataa ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc
    10501 gacgcgcatt gttgatctcg atcgcggcaa acttgtgacc tatccaggga attacgacca
    10561 gtacctgctg gaaaaagaag aagctctgcg tgtggaagaa ctacaaaatg ccgagttcga
    10621 tcgcaaactg gcgcaggaag aggtgtggat ccgccagggg atcaaagcac gccgtacccg
    10681 taatgaaggc cgcgtacgcg ccctgaaagc aatgcgtcgc gagcgtggcg aacgtcgcga
    10741 agtgatgggt accgcaaaga tgcaggtgga agaggccagc cgctccggta agatcgtttt
    10801 cgaaatggaa gacgtttgct accaggtcga cggtaagcaa ctggtgaaag atttttctgc
    10861 ccaggttcta cgtggcgaca aaattgccct gattggtccg aatggatgcg gcaaaaccac
    10921 gctgctgaaa ctgatgctcg gtcagcttca ggcggacagc gggcgtattc acgttggcac
    10981 caaactggaa gtggcttatt tcgatcagca ccgtgcggaa ctggatcccg ataaaacggt
    11041 gatggataac cttgctgaag gtaagcaaga ggtgatggtt aacggcaagc cacgccatgt
    11101 tttgggctat ttgcaggact tcctgttcca tccgaaacgg gcgatgacgc cggtacgtgc
    11161 gctttctggc ggtgagcgta accgcttgtt gttggcgcgt ttgttcctca aaccaagcaa
    11221 cttattgatt cttgacgaac cgaccaacga tcttgatgtt gaaacgctgg aactgctgga
    11281 agaactgatc gacagctacc agggcacggt attgctggtg agccacgatc gtcagtttgt
    11341 cgataacacc gttacagaat gctggatctt cgaaggcggc ggtaaaattg gtcgttatgt
    11401 tggcggttat catgatgccc gtggtcagca agaacagtat gtggcgctca aacagcctgc
    11461 ggtgaaaaaa accgaagaag ccgccgcgcc aaaagcagaa actgtaaaac gcagcagtag
    11521 caaactaagc tataaattgc agcgtgaact ggagcagcta ccgcaattgc tcaaagatct
    11581 ggaggcgaag ctggaagccc tacagacgca ggtggcggat gcttccttct tcagtcagcc
    11641 gcatgagcag acgcaaaaag tgctcgctga tatggctgct gcagagcagg agctggagca
    11701 agcctttgaa cgctgggagt atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag
    11761 tcgatttaaa cagagacagc tgacaacggt aaaattgcct gatgaactgg gttaatcagg
    11821 catgaaaacc aacactttgt tagtcatctg taaaatagcg catcattaag gagtaccaat
    11881 gtgcgaacat catcatgccg cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc
    11941 gttaccgcgc cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca caacgttaac
    12001 cgtggcgtgg gatgcccctc ggcagcgtcc caccgcctat gcgttggctg cactgttcat
    12061 gctgttgctg tccaacttgt ttccttttgt gaatatgaac gttgcgggag tcaccagtga
    12121 aattacatta ctggaaattc ccggcgtgct tttttctgag gactacgcca gcctcggcac
    12181 ctttttcctg ttgtttgtgc aactggttcc cgcgttttgt ctgataacca ttctgttact
    12241 ggtgaatcgc gcggaattac cggtccgttt aaaagagcaa ctggcacgag tgctttttca
    12301 actcaaaacc tggggaatgg cggagatttt cctcgcgggt gtgctggtca gtttcgttaa
    12361 actgatggcc tacggcagca ttggtgtcgg cagtagcttt ctcccctggt gtttattttg
    12421 tgtcctgcaa ctgcgcgcct ttcagtgcgt tgatcgtcgc tggttgtggg acgatatcgc
    12481 cccgatgcca gcactgcgcc agccgctaaa acctggcgtc acggggatac gtcaggggtt
    12541 acgttcgtgc tcctgttgta cggcaatcct tcctgctgat gaacccgtat gcccgcgctg
    12601 tggtactaaa gggtacgttc ggcgtagaaa cagcctgcaa tggacactcg cgctgcttgt
    12661 aacgtccatc atgctgtatc ttccggctaa tattttgccc atcatggtga cggatttatt
    12721 aggttcgaag atgccatcga cgattctcgc tggggtcatc ctgttatgga gcgaaggatc
    12781 ttatcccgtc gctgcggtta tctttctggc cagtattatg gtgccaacgt taaagatgat
    12841 cgccatagcg tggctgtgct gggatgccaa agggcatggc aagcgcgaca gtgaaagaat
    12901 gcatttgatt tatgaagttg ttgagtttgt aggccgctgg tcgatgattg acgttttcgt
    12961 tatcgcggtg ctctcggcgc tggtgcgtat gggaggttta atgagtattt atccggcaat
    13021 gggtgcatta atgtttgctt tagtcgtcat aatgacaatg ttttctgcta tgacgtttga
    13081 cccgcgtttg tcgtgggatc gtcaacctga atcagagcat gaggagtcct gacagtatgg
    13141 aatctaataa tggggaagcc aaaatccaga aagtgaagaa ctggtctccc gtgtggatat
    13201 ttcctatcgt cacggcgctc attggggcct gggttctttt ttatcattac agccatcagg
    13261 gaccggaagt gaccctgatc accgcgaatg cggaaggaat tgaaggtggc aaaaccacca
    13321 ttaaaagccg tagcgttgac gtcggcgtgg ttgaaagcgc cacactggct gatgatttga
    13381 cgcacgttga aatcaaagcg cggctgaatt ccggtatgga aaaattgctg cataaagata
    13441 ccgtcttttg ggtggtgaaa ccgcagattg gtcgagaagg gattagcggc ctgggaacgc
    13501 tgctgtctgg ggtttatatc gaactgcagc caggcgcgaa aggcagcaaa atggataaat
    13561 acgatttgct ggactcgcca ccgttggctc cgcctgatgc gaaaggtatc cgtgtgattc
    13621 tcgatagcaa aaaagccggg cagctctcgc caggagatcc cgtgctgttc cgtggctatc
    13681 gggtaggttc ggttgaaacc agcaccttcg atacgcaaaa acgcaatatc agctatcaac
    13741 tgttcatcaa tgcaccttat gaccgactgg tgaccagcaa tgttcgtttc tggaaagata
    13801 gtggcattgc ggttgatttg acgtcagcgg gaatgcgtgt ggagatgggc tcattgacaa
    13861 cgctgctgag tggcggtgtc agctttgatg tgccggaagg tctggattta gggcagccag
    13921 tggcaccgaa aacagctttc gttttgtatg atgatcagaa gagcattcag gattcgttgt
    13981 acaccgatca cattgattat ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg
    14041 gagctccggt agaattccgg ggtattcgcc tgggtaccgt aagcaaagtg ccattctttg
    14101 cgccgaatat gcgtcagaca tttaacgatg attaccgtat tccggtactg attcgtatcg
    14161 agccagagcg gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag caccttggcg
    14221 aattgttgaa acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc actggcgcac
    14281 tgtatgttga tctcgatttc tatccaaata cgcctgcaat aaccggtatt cgtgaattta
    14341 atggttatca gattatcccg accgttagcg gcggtctggc gcaaatccag caacgactga
    14401 tggaagcgtt ggataagatc aacaaactgc cattgaatcc gatgattgaa caggcaacca
    14461 gtacgctttc tgaaagtcag cgcacaatga aaaacctgca aacgacgctg gatagcatga
    14521 acaagatcct cgccagccag tcgatgcagc agttaccgac ggatatgcag tcaacgttgc
    14581 gtgaattgaa tcgcagtatg cagggcttcc agcctggctc cgcagcctac aacaagatgg
    14641 tggcggatat gcagcgactt gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc
    14701 tcaacgagaa gagtaacgcg ctggtatttg aagcgaagga caaaaaagat ccagagccga
    14761 agagggcgaa acaatgaaaa agtggctagt gacgattgca gcactgtggc tggccggatg
    14821 cagctccggc gaaattaata aaaactatta ccagttacct gtggtgcaga gcggtacaca
    14881 aagtaccgcc agccagggta atcgcctgtt atgggtagag caggtcgctg ttcctgacta
    14941 tctggcgggg aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa
    15001 caacttgtgg gccagcccgt tggatcaaca gttgcgcaac actctggttg ccaacctgag
    15061 cacgcaactg cccggctggg tggttgcctc ccagcctctg ggaagcgccc aggatacgct
    15121 caatgttacc gtaacggagt ttaacgggcg atatgatggc aaggtcattg tcagtggtga
    15181 gtggctgttg aaccaccagg gacaactgat caaacgtccg ttccgtctgg aaggggtgca
    15241 aactcaggat ggttacgatg aaatggttaa agtgctggcc agtgtctgga gtcaggaagc
    15301 cgcttctatt gcgcaagaga taaagcgtct accgtaatca taaagatttg taaatataac
    15361 cgtctccggt atgttgcctg aggcggtttt tttgtttcta aggtgcggaa aaagttgttc
    15421 ctcttcacat tttttgtaca actgacatgc ccgtgtagct cacaaatatg acagtggcgt
    15481 gaattttgcg cattgacggc agttatgatt cgcggtattg cttaactgtg attgcacatt
    15541 tagtaatcac tgttttcttt tccaccagaa accagtatga gggaaacgag gcatgaagag
    15601 acaaaaacga gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg
    15661 acgctcaaaa gaaatgtgtc cctatcagac gctgaatcaa aggtcacaat ggctgggagg
    15721 ctggcgagaa gccatggcgg acagggtagt aatggcctga ttctgtctct ttaaaaagaa
    15781 acctccgcat tgcggaggtt tcgccttttg atactctgtc tgattataat cagaaggcag
    15841 acgtatcctg gaacagaccg actttcaggt cgctggcggt atagatcaga cgaccatcaa
    15901 ccagcacttc gccatccgcc aggcccataa tcagacgacg gttaacaatg cgtttaaagt
    15961 gaatacggta ggtcactttt ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc
    16021 caacgcccag cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag aaccctacca
    16081 gctgccacat tgcgtccagg cccagacatc ctggcataac cggatcacca atgaagtggc
    16141 atccgaagaa ccacagatcc ggattgatat ccagttctgc ttcaacatac cctttgtcga
    16201 agttaccacc cgtttcggtc attttgacca cacggtccat catcagcatg ttcggtgctg
    16261 gcaattgcgg acctttagcg ccaaacagtt caccgcgacc agaggcaaga aggtcttctt
    16321 ttgtatagga ttcgcgttta tctaccatgt tctctgtaag ccttatttta ttgaagcacg
    16381 caggatagct aacacgtgta cgctgaacaa gtccgatcag ttcggaataa accagttcag
    16441 ccaacgtaat ggccatggaa aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg
    16501 ttcctggatg gtttgcatca gcgtcgtttg gccttcgcca tcccacacca gatttaataa
    16561 taacggcagt gcgtcagtca catcgtctac tgcccagatg gtgaatttgc cttcttctac
    16621 cgctttcacc agttcactgt gaagacttaa atggcgaatg ttagcagtgg ggataatgac
    16681 accttgtttc ccagttaact cacgttgctg gcaaatagca aagaagcctt cgattttctc
    16741 atttaaacca ccgaccggct gggcacgacc gaactgatcg actgaacctg tgatagcgat
    16801 actctgattc accggcacat cggcgagggc gcttatcagg gcgcagagtt cagccatcga
    16861 ggcactatcg ccatccactt cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg
    16921 gatctgttgc tcaagctgta gttccgacat caggaacgcc tgcatgatca tcatcccttt
    16981 cgcatggata ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc
    17041 aatatgcaca acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa
    17101 ttcaatgacc gaaagggcgt taatttgccc gatgcgttcg ccttcggttt caatcaggat
    17161 ttgctcctga agaatctcat cctgcatgcg ttcagcgagg aaaccttcgc gccattcacg
    17221 ctgctgtagc attaagttta gctgctcgcc ggagaaggtg tcgccatcac acagggaggc
    17281 gacctcttta cactggcgga ggatccactg cgggctaagc ggaagtgttt cttgttcacc
    17341 ggtgtagcgt gctgcttcgc ggataagcac cggccaggca tccgctcccg gtgcaggtaa
    17401 gtgattatgt ctggcggtaa atgtcaccca gcgacaccac tggcttactg actccgcatc
    17461 gacaatctgc agagtatctt caaattcgct ataaatagcc tgctctgaaa gctctggctc
    17521 catctcctgg aaatcagcca atgattcgcg ttcgcctacc agaatgactt tcagcttcaa
    17581 tggcatcgaa ggcacagaga cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa
    17641 acgcccgcgg ttaacgatat ttttcagccg catccacagc agaggttgcg ccagcagtgt
    17701 acgcaaagag atgatgagaa taccgccatt tgcctgatgc accagacctg gctgcagggt
    17761 aatgtcgcca ttaaactggc gcaggcagcc aaagagttgc tccgcttcta cccagtcggc
    17821 agcgacaact tgcgttaaag tcgcaaaatt atcatctgca ctcactgtgt tacgtaagcg
    17881 gatggtgtgc ccggaaacct catagtgacc gcccaccagt tggcctgcat cgctttgtag
    17941 cgcacgcgcg gcatcggcaa taagattcag atactcagac tcttccgggg ccttcgccag
    18001 cataaaagag gaggatgctc gcgtatgcag caactgctcc agcgcaaatt gcagtcgcgg
    18061 ttgagtatca ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag caaatatttc
    18121 ctgatagcta tcggtatcag gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt
    18181 tgatgttttt tagtctgttg tcaaagccgc gattataccg taaccggcac tgcagcacac
    18241 gtagaaagca ccgacaatac tcctggcatg ggcgttaaag ctcacaggat ggagattctt
    18301 ttcttcactg gcctaaaaag ctgatattct gtaaagagtt acacggtaac attgagatcg
    18361 ctatgaaata tcaacaactt gaaaatcttg aaagcggttg gaaatggaag tatctggtga
    18421 agaagcatcg cgaaggggag ttaatcaccc gttacataga agccagtgcc gcccaggaag
    18481 ccgtcgatgt gttgctctcg ctggaaaatg aacccgtgct ggtaaatggc tggattgaca
    18541 agcatatgaa tccggaactg gtcaatcgca tgaagcagac cattcgggcc agacgaaagc
    18601 gccattttaa tgcggaacac cagcatacgc gcaaaaaatc gatcgatctg gaatttattg
    18661 tctggcaacg tctggctggt cttgcgcagc gtcgcggtaa aacgctttct gaaacgattg
    18721 ttcagctgat tgaagatgcg gaaaacaaag agaaatacgc gaataaaatg tcttctttga
    18781 agcaggatct gcaggcattg ctgggtaagg aataactgac gaaagtcagt tcaattttct
    18841 aaagacgaaa aaaaaccccg cagcagcggg gttttttacc agacgagaac ttaagcctgc
    18901 ggctgagtta caacgtcttt gatgccttta acttcgatct ctacgcgacg atccggagcc
    18961 aggcagtcga tcagtgcagc acgctgtttc acgttgtcac aggtgttgcc agtaaccggg
    19021 ttggattcgc ccataccacg tgcggagatc ttgtctgccg ggataccttt ggagatcagg
    19081 taatcaacaa cagactgagc acggcgctcg gacagaccct ggttgtaagc gtcagaaccg
    19141 atgcggtcag tgtaacccag aacaactacg gaaccgtctt tcggatccag gttgctcagc
    19201 tggctgtaca gctgatccag agcagcctga ccttccggtt tcagggttgc tttgttgaag
    19261 gtgaacagaa cgtcagactt cagagtgaag tgcttggtct gtacttccgg tgccggagct
    19321 ggagccggag caactactgg agctgcttcg ccctgaccga aacggtagga aacacccagg
    19381 ctcagcatgc cgttgtccgg acgagtaccg atggtgtgtg cgtcaccgat gttgttggtc
    19441 cactggtatt ccagacgggt agcgatttca ggagtgatcg catactcaac accgccagcg
    19501 aagaccggag aaacgccggt gtcatggttt ttaccataaa cgttggattt agtgtctgca
    19561 cgccatacca taccacccag acgagtgtat acgtccagat cgtcagtgat tgggtaaccc
    19621 agtttagcgg tcagctgaac gccctgagct ttatatgcac cgttttcaac gctgcctttg
    19681 tatggcatac gacctaacca gtcataaccc atttcaaagc caacatacgg gttaacctgg
    19741 taaccaccaa aagcaccagc gcccagttgg ttttcatggg tcggaccatt gttgttgatg
    19801 aaaccagtgt catggtactg ggaccagccc agtttagcac cagtgtacca ggtgttatct
    19861 ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct
    19921 gtctttttca ttttttgcgc ctcgttatca tccaaaatac gccatgaata tctccaacga
    19981 gataacacgg ttaaatcctt caccggggat ctgctcaata ttaactctac cgatatcttc
    20041 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    20101 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtacgttta atcttttttg
    20161 tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    20221 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    20281 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg
    20341 agaggatgca cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    20401 aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacgct
    20461 gtaattgccc gtgcgtaaag cgcgaaccat tgactccacg gtgtggcaag gggagagctg
    20521 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cgcgactcag
    20581 tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa
    20641 tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat
    20701 aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa
    20761 cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta
    20821 ctgtatggat gtacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc
    20881 ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa
    20941 gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt
    21001 ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc
    21061 tggcaacgtt gggcacaatt gaataccgat cattgtttgg tagttacagc ctgaccgttg
    21121 acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa
    21181 gtgcacagta ctgtgtaaaa catccgcctg tttggctgac atataaaaag tgtggccgat
    21241 ccgttaccct caattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg
    21301 tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc
    21361 gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagg
    21421 tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt tggttgcgac
    21481 tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa ggggccatta
    21541 tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca gaatgggctg
    21601 actctcttac gccgaaacag gagtttcctg cggaacttga gtaatctggc gttgcagacg
    21661 accaatctct ggcagtaatg caatcaataa cccaacttgt tgtacgacca gaggttcttt
    21721 gctgtctgcc cgaggttcaa gttgctgcat ccgctgtttc aggccagcta atgcctgatt
    21781 gacgcgttct tcatcagcag gttgatgatg tagcgcgtca tcaacatagc aaacggcgtc
    21841 atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga
    21901 gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt cgcgaatttg
    21961 cggggtaacg ttcggttcgc tggacatatt tgataccacc gacgccagtt cagcatcacg
    22021 gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta
    22081 ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt cgagcatgcg
    22141 cggcagattg cgaaacttcc agtcaggcca gatgtagctc accgccgccc acgcaatggc
    22201 acaaccaatc agcgtatcga ttacgcgagg taacgccact tcaaaacctt cacccaacaa
    22261 gttaaaacaa agtagcacca aaagtgtgat gaacatcgtt gcatgggcgt attgcacgtt
    22321 acggaaggca aaaaagagca cgccggtaat aaccagtagc accagttgcc cttccagtga
    22381 cggtacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac caataatcct
    22441 taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag
    22501 caagatccaa tacccgtgat gcattccggt tatctgaatg atggcgtagc cgaagcacaa
    22561 caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct
    22621 aagacgcagc cagatatcac tcaatccgtg cgggctgtca tcagcgagct cattttcgtc
    22681 attattatgg ggcagtgcct gggcctgttc tgattcaatt gttgccagtt gggcatcaat
    22741 ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcag gtgcgccgtt
    22801 atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcga aatgcggatc
    22861 atgttgataa ggctgacgca gcaaaataca gcgtgacagt tgctggcacg cctggccctg
    22921 catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa
    22981 tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgcg cgacaaagta
    23041 ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag
    23101 cgagagtttc gtctgattca atgtcgccat cagctgaccg ttggcgagag ccaaatcgta
    23161 cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgtgact tgagctcaag
    23221 gtaacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa
    23281 cagtagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat
    23341 cggctgctga taccagtgct catacagtga cgtccccaac atagtgtaaa tggcgatcag
    23401 taacgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc
    23461 gctggtagag agcgttaagc caattgcaaa tagccagggc cagggaaaca gcaattctac
    23521 tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc
    23581 cagccggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtca gcggaatcgt
    23641 cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat
    23701 acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag gacttagcat
    23761 aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg
    23821 cgctgtttct accgatacca cgcgacgacc gaccggccat agtgcgatag cggcaatttt
    23881 aaagttcgca atgccgacag gaatgccaat gatggaaata cattgtgcga tgcccgttgc
    23941 aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt
    24001 accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga catgaatagc
    24061 ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg
    24121 taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca gagtggtggc
    24181 aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac
    24241 tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc
    24301 tgacggttac actgataaga aatcatttcg tgtggatcta cagagtcatg gaactgaaag
    24361 cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg
    24421 ccgggattaa agtctccggt gatcgccacg aataccttat tcctttcaat caattactgg
    24481 cgattcactg taagcgcggt ctggtatggg gcgagctgga atttgtactg ccggacgaaa
    24541 aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac catcatcttg
    24601 atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttactcc
    24661 agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa
    24721 cctctggcat tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccggttaacc
    24781 gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag
    24841 atattgaaag cgctcggttg cagcataacc aggcgtatac agaagccatg cttaccgagt
    24901 atgcagattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag gcccgggcag
    24961 tcgttaatgg cgagcattct ctgttagtgc tggctggtgc aggaagcgga aaaacgtcgg
    25021 tgctggtggc ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct gagcaaattt
    25081 tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcgtatt cgcgaacggc
    25141 tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat attattcagc
    25201 agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct gcccgtcatg
    25261 aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct
    25321 ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg
    25381 atgaaaaatt acagcgtcgt cttgcctcac gcctcgatcg ttgggtaagt ctgatgcgga
    25441 tgcacggtgg cgcacaggca gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt
    25501 tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt gcgctgaagg
    25561 cagaaaacgc tgtcgatttt tcgggcctta ttcaccaggc gattgtgatt ctggagaaag
    25621 gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat atctcgccgc
    25681 agcgcgcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg ttgttcgccg
    25741 ttggtgatga ctggcaggcg atttatcgct tcagcggtgc gcaaatgtcg ctcaccaccg
    25801 ctttccatga aaactttggt gaaggcgatc gctgcgattt agacacgact taccgtttta
    25861 acagtcgtat cggtgaggtg gcaaaccggt ttattcagca gaacccaggc cagctgaaaa
    25921 agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg gatgagagcc
    25981 atcttgacgc tttgctggat aagctctctg gttatgccaa accggaagag cgcattctga
    26041 tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca acacgctggc
    26101 cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag gcggattacg
    26161 tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg cgggagtcga
    26221 ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa gaacggcggt
    26281 taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt aacaaagaga
    26341 atccctctcc ctttgtggaa atactgaaaa atctggatgt gccggtggcg agaaaaccgt
    26401 aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg cgagataacg
    26461 ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact ggataatgaa
    26521 gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca gacgcagcaa
    26581 ggctttcacg tcaggatcgt gcggaacggc atttagtggg tcccagaaga aaatcaatac
    26641 atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca ttgggccact
    26701 caacatcgcg ttgacgttca tgctggttgc acgggaaatc aagttaccgg tagtgcctgt
    26761 tgcatacagt acgtgttgtt ccagtaacgg ttgatgtcgt tccacccagc tcatcagcat
    26821 ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta aagtgcgtgt
    26881 cgtcagttcc ataatgtaca tccgtagtta actttcctac agattactgt aagcacttat
    26941 cgctgcaaga gaaagaccga aaaagcctgc gcacaggcac aaaaatctca ggaagatggt
    27001 tgtttttccg cccattgcag gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa
    27061 tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc cagctcggaa
    27121 acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg
    27181 cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt
    27241 gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc atccaacaat
    27301 tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag acgaggcagg
    27361 cgaaaattga cctggttgat gagctgggta ttaaaactga ccaccagcgg tggggagata
    27421 tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa cactaactga
    27481 tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct ggaaaccttt
    27541 ttaccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg
    27601 gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat aggtagctcc
    27661 tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat acttactaac
    27721 atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat ggttggcatg
    27781 tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga aaccgatatt
    27841 gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga caaacccgat
    27901 cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt cattccggtt
    27961 tcaccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac gctggcagaa
    28021 gttccggaaa aagtcgatat ggtcgatgtt ttccggaatt cagaagcggc gtggggcgtg
    28081 gcgcaggaag cgatcgccat tggcgcgaaa accctctgga tgcaactggg ggtgattaat
    28141 gagcaggcgg cagtactggc acgggatgcc gggttaaatg tggtgatgga tcgctgcccg
    28201 gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag gccaaaacct
    28261 tccggggatt tgttcaggga ttagttacgc agacgcgggg cctgaagttg tttgcggatg
    28321 gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa ttcgctactt
    28381 agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg
    28441 tgataccacg gagcggcgcg aagctcgtca ttcaccgcca gctcatcagg cgacggttca
    28501 gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa cagggaatgg
    28561 cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg ggaaatcttt
    28621 acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca ggcaaaccct
    28681 ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc ctgacggaac
    28741 tgctctataa attgtacatc acgaccggca tcaattgcgg catccgcgat gattttctga
    28801 aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaactcc accttcattc
    28861 agcaactgaa tcgccagcat gttgatgtct ttatagccac gacatgcgcc catcaactgg
    28921 cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga
    28981 tcgcgataag tacgcatcaa tttaaagaca tcatcacgga caaactcagc cttgctcaga
    29041 tccagtttgt tcagctcaac gttctgccgt gcaatatcca gggcttcctg ggaggtatca
    29101 acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc accggtatag
    29161 gagaaacagt tcagtacgcg tttattttca acgtagcggc gggtagccag gcggctatca
    29221 cgctgatcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag cagcttcatg
    29281 ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacggg gccctgggtc
    29341 agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat cgcacattcc
    29401 gggtacagcg tttgcagggc gctaattaat gccgcgcgct gatattctgc gccagcactc
    29461 agtagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg caggccatca
    29521 gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag ccagtcacgc
    29581 catttttgcg cttgttgcaa acggcgggta aaaaaagcaa tgtcgataga ttcattcggg
    29641 tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc gcgtgctaac
    29701 cattttccct gatgatcaac aatatcgatg gtttccccga ggctggcttt accttccatg
    29761 cgggcaacgg ccccggaaaa gatccacgga tggcgacgaa gtaatgattt ttcgcgccct
    29821 ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa gaaatgggcg
    29881 tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag gagagcagca
    29941 atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt tggatttcgc
    30001 tataccacgc agtacgaagc gaaaaaacta ggattagccg ggtacgccaa aaatcttgac
    30061 gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtagaaaa attaatgcag
    30121 tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat
    30181 catccctcgg gggaattaac cgattttcgt atccattaaa tacattttac cggttttggc
    30241 aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg gtataaatag
    30301 cggctgttac ctttttcttc accaaattta ttcgccatcg cttttaccag catacgaatc
    30361 gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt
    30421 tctggcgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg ctcactccac
    30481 tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc ttcgaagatc
    30541 agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac aaagccccgc
    30601 ataaagcgag gctcggtaag taaccctggg cgagattaat cgcggctggc gaagcccaga
    30661 atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac tgtggcacga
    30721 atatagttcg tttcaccgcc atgaatgatg ttgctggttt caaacaaaat agcgccagag
    30781 gagatcagaa tgaagaccgc gctgatcgcc agatgcagag caggcagttg caggaagata
    30841 ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc gccgaggaac
    30901 gacatatctt tgcgggtggt cagcacatac gcagagcagc agaagaacac caacgccgtt
    30961 ccgcccagtg ccatagcgat tacgtcaccc attccggcgg acaggtaggt gttcagaatt
    31021 ggtccgagga tatatcccag aaaaccggta aaggcgaatg cggagataat cccggtcggc
    31081 ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag cgtcagaatc
    31141 agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc
    31201 agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct aagcagcgat
    31261 gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat gacagatgta
    31321 attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac ccatctttac
    31381 gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa atataacgcc
    31441 ctgagaattt cgacaggcaa aagaaaaagg ggttagcagt tagctaaccc cttatcttat
    31501 ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc
    31561 ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac atcccgatgc
    31621 ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc
    31681 gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt tactcgttta
    31741 gcaaccggct aaacatcccc actgcccggc aaaaagaaaa ataggtccat ttttatcgct
    31801 aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa
    31861 cattactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt cagcatctca
    31921 cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa gcgcccggtg
    31981 tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa acattttacc
    32041 aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt agtctggctg
    32101 ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg ctggagaaca
    32161 aaccgcgcat tccggtggta tggatccacg gtctggaatg cacctgctgt accgaatctt
    32221 ttatccgctc cgctcacccg ctggcaaagg acgtcatcct ttccctgatt tccctcgatt
    32281 acgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttt gaagacatca
    32341 tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg ctgggcgagc
    32401 aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa cgtgccgctg
    32461 ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc gtgcaggccg
    32521 cgcgacccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac aaacctatta
    32581 tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt acttacatgg
    32641 tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgttgatg ttctatggtc
    32701 agcgaatcca cgataaatgc tatcgccgcg ctcacttcga cgccggagag ttcgtccaga
    32761 gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc
    32821 ctaccaccta taacgcctgt tcctccacac gctggaacga tggcgtttcc ttcccgatcc
    32881 agtcaggtca cggctgcctg ggttgtgcgg aaaatggttt ctgggatcgc ggttcgttct
    32941 acagccgcgt ggtagatatt ccacaaatgg gtactcattc caccgccgac accgtcggtt
    33001 taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg
    33061 accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca ggcaatgagg
    33121 ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata atgccggacg
    33181 ccgcctggtg gtcgacccga ttacgcgcat tgaaggccac atgcgctgcg aagtgaatat
    33241 taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc gcgggctgga
    33301 gatcatcctg caagggcgtg acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg
    33361 cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg ccatcggtat
    33421 taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc tctggtgcca
    33481 cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg tgttagatgc
    33541 gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct cttggccgaa
    33601 atcatcccct ggctatttct tcgacgtaca aaatcgcctg aagaaattcg ttgaaggcgg
    33661 ccagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac tgccgccaga
    33721 agccaacctg atgggctttg cccactatct cgaagctctc gatttccagc gtgaaattgt
    33781 caaaatccac gctgtctttg gcggtaaaaa cccgcatcca aactggattg tcggcgggat
    33841 gccttgcgcc attaacattg acgaaagcgg cgcggtcggg gcggtcaata tggaacgcct
    33901 gaacctggtg cagtcgatta tcacccgcac ggcggatttc attaacaacg tgatgatccc
    33961 cgacgcctta gccattggtc agttcaacaa accgtggagc gaaatcggca cgggtctttc
    34021 tgataaatgc gtcctcagct atggcgcatt cccggatatt gccaacgact ttggtgagaa
    34081 aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg tgctgccagt
    34141 ggatttggtt gatccgcagc aggtgcagga gtttgtcgat cacgcctggt atcgttatcc
    34201 caacgatcag gttgggcgtc atccgttcga tggcatcacc gacccgtggt acaaccccgg
    34261 cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac gctactcgtg
    34321 gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg cacgcacgtt
    34381 aatcgcttat cacaaaggcg atgcggcgac cgttgagtcg gtcgatcgca tgatgtcagc
    34441 gttgaacctg ccgctttccg gcatccagtc aacgttgggc cgcattttgt gtcgcgcgca
    34501 cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gataagctga tgaccaatct
    34561 gaaaaacggc aatctcgcca ctgcttccac tgaaaaatgg gaacctacaa cctggccgac
    34621 agagtgccgt ggcgtcggct ttaccgaagc gccgcgcggg gcgttaggcc actgggctgc
    34681 cattcgcgat ggcaagattg atctctatca gtgcgtggtg ccgaccacct ggaacgccag
    34741 cccgcgcgat cctaaagggc aaattggcgc ttatgaagcg gcgctgatga acaccaaaat
    34801 ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg atccgtgcct
    34861 cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctccg tgcaggtgcg
    34921 ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca gtcactatgt
    34981 ctttgaagca cccgtgcgga tatggcactg gctgacggtg ttatgcatgg cggtgttgat
    35041 ggtcaccggt tactttatcg gcaagccgct accttccgtc agcggcgagg cgacgtatct
    35101 gttctatatg ggctacatca ggttaattca cttcagcgcc gggatgattt ttaccgtggt
    35161 cttgctgatg cggatctact gggcttttgt tggcaatcgt tactcccgcg agctgtttat
    35221 cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc gctggtatct
    35281 gtttctggca aaacgtccga gtgccgatat aggccataac cccatcgccc aggcggcgat
    35341 gttcggctat ttcctgatgt cagtttttat gatcatcacc ggctttgcgc tgtacagcga
    35401 acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt tctactggac
    35461 gggcggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc tgattggcgc
    35521 gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg acgacacagt
    35581 gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa gtaacaagga
    35641 gcgttcatga gcgagcaacg cgtggtggta atggggctgg gcaatctgct gtgggccgat
    35701 gaaggcttcg gcgtgcgggt ggcggaacgg ttgtatgccc attaccactg gcccgatgaa
    35761 gtcgagattg ttgatggcgg cactcaggga ctgaacttgc tggggtatgt cgaaagcgcc
    35821 agccacttgc tgattctcga cgccattgat tacgggttgg aacccggaac gctgcgaaca
    35881 tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct gcaccagaac
    35941 agcttctccg aagtgctggc gctggcggat atacgtggtc atttgccagc acatattgcc
    36001 ctcgtcggtc tgcaacccgc aatgctcgac gactacggcg gtagcctgag cgaactggca
    36061 cgggagcgac tgcccgcagc ggaacaggcg gcgctggcgc agctggctgc atggggaatt
    36121 gtgccgcaac cggctaatga atcgcgctgt ctcaattatg actgtctgtc gatggaaaat
    36181 tatgaaggca ttcgcttgcg ccagtaccgg atgacgctgg aggagcagag atgagcaacg
    36241 acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg ccggtcagtg
    36301 aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta ttaagcagtg
    36361 acccgaaacg cacgccagaa gtcagcgata acccggtcat gattggcgaa ttactgcgcg
    36421 agtttcccga ctatacatgg caggtagcga ttgctgatct tgagcagagc gaagccatcg
    36481 gcgatcgctt tggcgtcttt cgctttcccg ccacattagt gtttaccggc ggaaactatc
    36541 gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg cgcgggcttg
    36601 tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc tggggccagg
    36661 aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct gtcaggtgaa
    36721 tggtgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg tgattgcgct
    36781 gttaaacgag ttacaacatc aactaagcga acgccagccg ccgctgggtg aggtactggc
    36841 agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc ttctcgggga
    36901 aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt gaaagtgaaa tacaggaggc
    36961 gatcttctgc ggattatggc gggtgcgcag acgttgcggc gaacagttgc tggaggacaa
    37021 actggaggct ggctgcgcgc cgctggcatt gtggcaggcg gcaacgcaaa acgtcttgcc
    37081 gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac cgttggctca
    37141 tgaattactg gcacatgtac gtaatcctga cgcgcagccg cacagcatta atctgacgca
    37201 attacctatc agcgaggctg atcggttttt tctctcacgt ctctgtgggc cgggaaatat
    37261 tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt tacgccatgt
    37321 ctggcattta cgctgtacgg acaccttaaa aggcccttta ctggaaagtt atgaaatctg
    37381 cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg cgcagcggct
    37441 tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg gtaagtatcg
    37501 ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt ttcaggataa
    37561 agagggagat ctaccattat cgggttattt ttctctcttc gcctacagga gtgcgcatgt
    37621 gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat cactttttat
    37681 ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc atttatgtgg
    37741 tcaccggcaa aacgatctac cgcgatatga cgcgcttctg gggtaagctc ttcggtatca
    37801 actttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt actaactggt
    37861 cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg gaagcattaa
    37921 tggccttctt cctcgaatcc acctttgtcg ggctgttctt cttcggctgg caacggctga
    37981 ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat ctctctgcgt
    38041 tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat tttgatatcg
    38101 acacccttcg tatggaaatg accagcttca gcgagctggt ctttaacccg gtcagccagg
    38161 tgaaatttgt gcacaccgta atggcgggct acgtgaccgg ggccatgttt attatggcga
    38221 tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc tcgtttgcca
    38281 tcggttccgt cttcggtact ctggcgatta tcggtacgct gcaactcgga gatagttcgg
    38341 cgtatgaagt cgcgcaagta caaccggtaa aactggcggc aatggaaggg gagtggcaaa
    38401 cggaacctgc acctgcaccg ttccatgtgg ttgcctggcc ggaacaggat caagagcgta
    38461 acgcctttgc catcaaaatt cctgcgctgc tagggatcct cgccactcac tcattagata
    38521 aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg caacgcggac
    38581 gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc
    38641 aggcattccg ggagctggaa ggcgaccttg gttacggcat gttactctcc cgctatgcgc
    38701 cggatatgaa tcatgtcaca gctgcacagt accaggcggc gatgcgtggc gcgatacctc
    38761 aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttct ctgctgctac
    38821 tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aattgaccag catcgctggg
    38881 tgctgaaaat ggcgctctcg agtttgccgc tgccgtggat tgcgattgaa gccgggtggt
    38941 ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg acatactccg
    39001 cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc gtagggcttt
    39061 acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt ctggggccga
    39121 gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa tcatgtttga
    39181 ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg tggtctttat
    39241 gatctccgat ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg cgcgtaatga
    39301 tgatgaacgc cggatagtga taaacagcgt tggcgcgcac tgggaaggca atcaggtctg
    39361 gttaatcctc gcaggtgggg cattatttgc cgcctggccc agagtgtatg cagcggcgtt
    39421 ttccggcttt tatgtggcga tgattctggt gctgtgctcg ctgttcttcc gcccgctggc
    39481 cttcgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg atgccggact
    39541 ggtcatcggc agtctggtac cgccggtagt cttcggtatt gccttcggca acttgctgct
    39601 cggcgtgccg tttgccttca caccgcaatt acgcgtggaa tatctcggca gcttttggca
    39661 actgctgacg ccattccctt tattgtgcgg attgctcagc cttgggatgg tgattttgca
    39721 aggtggcgtc tggttacaac taaaaactgt tggtgcgatt catctgcgtt cacagctggc
    39781 gaccaaacgc gccgcactgc tggtgatgct ctgctttttg ctggcgggtt actggctgtg
    39841 ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt ccaatccgtt
    39901 aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg aatcgcccgt
    39961 tctgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga tggcgattta
    40021 tcgtggtcgc ccgggttggg gatttttgat ggcgtcattg atgcaatttg gcgtgatttt
    40081 cacggcaggc atcacgctgt tcccctttgt catgccgtca agcgtgagtc cgatctccag
    40141 cctgacgttg tgggacagta cttccagtca gctgacgctg agcattatgt tggtaatcgt
    40201 gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca aaatgtgggg
    40261 gcgcatgacg acagaaactc tccgccgtaa cgaaaacgag ctgtactaag gagcaggaac
    40321 aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt
    40381 gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taagcctgat gaggcgcatt
    40441 agcgtcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata tcgatgaaag
    40501 cgatcttgat cccattctta tctcttctga ttccgttcac cccgcaatct gcattcgctc
    40561 agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg
    40621 ccccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg ccaacatggc
    40681 cggtaaaact gggatggctg acaccgcgcg gtggtgagct aatcgcctat ctcggacatt
    40741 accaacgcca gcgtctggta gccgatggat tgctggcgaa aaagggctgc ccgcagtctg
    40801 gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc gaagccttcg
    40861 ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc
    40921 ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataac gcgaacgtga
    40981 ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg catcggcaaa
    41041 cggcgtttcg cgaactggaa cgggtgctta atttcccgca atcaaacttg tgccttaacc
    41101 gtgagaaaca ggacaaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga
    41161 gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggaaatat
    41221 ttctcctgca acaagcacag ggaatgccgg agccggggtg gggaaggatc accgattcac
    41281 accagtggaa cacgttgcta agtttgcata acgcgcagtt ttatttgcta caacgcacgc
    41341 cagaggttgc ccgcagccgc gccaccccgt tgttggattt gatcatgaca gcgttgacgc
    41401 cccatccacc gcaaaaacag gcgtatggtg taacattacc cacttcagtg ctgtttatcg
    41461 ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc
    41521 caggtcagcc ggataacacg ccgccagggg gggaactgct gtttgaacgc tggcgtcggc
    41581 taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta cagcagatgc
    41641 gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg accctggcag
    41701 gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg caaatcgtga
    41761 atgaagcacg cataccggcg tgcgctctcc atcaagacaa ataaacattt gccgctgttg
    41821 gtcctcgcag gccatttacg cggcaaaatt ccacacgtaa tcctggtata agcacttctg
    41881 cgtcgcgggg agtgaatgcg gaaatatgga cctggacttc tttacgaccg tcggagggga
    41941 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
    42001 acaaacaaat tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc
    42061 cagcgatatt taagaccgcc ggacggctaa aataaaattt gattaatctc aattatcatg
    42121 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
    42181 tctcattatc aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc
    42241 tgtattaaag ccctgtttgc caaaaggccc aaaatgaagg aagtaaaata tgtctaataa
    42301 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
    42361 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
    42421 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gtcccgcggc
    42481 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
    42541 catgttgtca tggttcacag taccgcacat cagcattcga tgtgacggag cgaaatcctt
    42601 taggggcaaa gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc
    42661 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
    42721 gcctttttta tatcagcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
    42781 ttgattattg aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg
    42841 ggaaccaaca ccttactggc gtgttgctgt cttttaagac cagaagaggt taacagtgaa
    42901 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgct gactttatcg cgcaaaaaat
    42961 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga
    43021 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
    43081 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgcccaatc attggcgctt
    43141 ggggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
    43201 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
    43261 acaacgcaaa ggctttttta tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg
    43321 ctaccggaca tgactctacg caagtcatac aaccggtaca ttccacggtt cgtacctgaa
    43381 ttaatttatc gaccgggatc cgcgatgggc aattttttgc gcatttgcca caatcgatac
    43441 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
    43501 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacagactg acaatcagca
    43561 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
    43621 cgtccatcac cacgctgtag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
    43681 ataacgcgat atagagaaaa aaactcaaca gcaaatactt cacgccgcgc agaggaatat
    43741 ccagccagcg gggaaggaca aattgccgac caaacagttt gttaccgaga tcgccgatta
    43801 attcagaaag cgtaccaacc gggcataacc atgaacaaaa ggcctttttt agtaatagac
    43861 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactggc
    43921 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatgc
    43981 ctcctggcct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
    44041 agaacgtgat ggcaatataa atattaattg ccaacagtaa taattgcgtc gctttacgcc
    44101 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
    44161 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
    44221 aaatgccact attctagttg ttcttaactg gctgatattg attcaaatcg tgttcaggtc
    44281 tttcttatta accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg
    44341 ggggcagata atggctgctg ctccagttgt gcgcatagct ggctggcgct acgcattccc
    44401 agactcgagc aactgctttt tagctgatgc gcggcacgct ttattttctc gctatcctca
    44461 ctggcgcggg caatgtcgat gtcatcgaga agcggcaggg catgttgtgt aaataatacc
    44521 agccattcgt ggatcttctc cgtccccatt aactgagcat cttcattgag ttgcgataca
    44581 tccagcggtt gttcgttatt agctggcagt tggagatagt gcgccagtaa ctgaccgagc
    44641 acttcacgcg gcaccggttt agggataatc ccgcgaaata gcgaactggt acgctggcgc
    44701 aacgtttcgt cgatgacatg ggcgctaaag ccaatcaaaa ccagcgacgg atattgctgt
    44761 gccagttgtc gggcaagcgt aatgccgtcg atatccggta gatcaaaatc caccagtgcg
    44821 gcagcaaacg gttcgctatt ttgcagtgcc tctaaagcct gcgcggcatt gccaacagca
    44881 acgacctgcg caccactgtt gttcagcatc tcaacggtaa ttcgctgggt tagcgggtta
    44941 tcttcaatta acagcaaacg taaaccgtgc agacacaccg cctgattgac tgtttttggc
    45001 acgggggcag tggcaacacg taacggcaag cgtaaacaaa aacagcttcc aacctccggt
    45061 gtgctggtgg cgctcagttc gccgcccatc gcctgggcca gacggctact gatagtcagc
    45121 cccagcccgg tgccgccgcg tttgccgcta acctgaacaa atggctggaa gatttcagcc
    45181 agtttcgtgg gatcaatacc gcagccgctg tcttccactt cgaccagcca ttgttcgcca
    45241 tcagtgcgac tacgcaggat aatgtgccct tcgtcggtga aacgcagagc gttgctcaac
    45301 aggttggtaa tgacctgacg aatacgccgc ggatcgccca ttaacgcagt cggtacatcg
    45361 tcggcaattt ctgttgccag gtgaatcggg cgacctttca cccgtccgct cattaattgc
    45421 agggtacttt ccagcagcgg gcgcggttca aagggttcat cgctgaccga aacattcttg
    45481 ccgcctgctt cgatagccga ataatcgaga atatcgttga ggatggtcag caacgattca
    45541 ccagagtcag taattgcgcg caaatcatcg cgctgggcgt taagtgcggg gttatctgcc
    45601 agcagctgtg cggtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc
    45661 gccagaaacg ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt
    45721 tctatcacca gttcctgcaa ttcagctgta cgcgctttga cctgtgccgc cagctgttcg
    45781 cggtggcgat tcagtgcatg aacactgctg cgaaatgcat ccatcagccg cccgatggtg
    45841 tccagctccc gcacgccagc ggtttccgga aaaggggagt cgatatcacc gtccagtaag
    45901 cgttgcagcg cctgcgtttg ttcggcaagt ggacgcgtga ctgagcgata aaccacgcgc
    45961 cagaggatca gaatcagtgc gcaaagtgaa accatcccca gcaataacag gctgtactgc
    46021 ccgcgtgcac tggctttttc cagatgcgcc agaccgtgct gattacgcag ctcaatggtg
    46081 tcgaccagtt gactgacttc gctactaaac tgcgcgaact gggcgatgtt attttgcgtg
    46141 agagtttgca ggcgatgact gatttcactg tcctgctgat acagcgccag caaatcgcta
    46201 tattggctaa cggaagttaa cgttgttgca acctgagcac gaactcccgg atcttcaatg
    46261 cgtatttgcc gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggc
    46321 gcatttttct ggatctgttc cagccccaga ttcatcacca tttgctgcac ccgcagggcg
    46381 ctaaggcgca gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg
    46441 agtgcacttt ctgcggcctg acgctgatgt tgttcgatca aatcgtaaat cccggcctgg
    46501 gtcgctccgg cggaggttgc cgcattattc gcctgacctt gcgccaggcg tgcgatctca
    46561 tcggcggcgg cgactatctg ctgactgagt cgctgttgtt gctggcgcag ttgcaaacgc
    46621 tgccccacca gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca
    46681 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
    46741 ctttgtgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
    46801 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
    46861 atggtaggaa tcaatgcctg agtgttgtct ttttccacct ggctgataaa gcgcaggttg
    46921 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
    46981 agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg
    47041 caaagtggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
    47101 ctatttttac ttctttccct tttcatgttg tcggcatttt cggctgataa cctgttgcgc
    47161 tggcatgatg cgcagcatta cacggtacaa gcctctacgc cgcttaaagc caaacgcgca
    47221 tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat
    47281 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
    47341 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
    47401 gccattttgc tcggtagtag cacgacgtca tttcccgacc tgcaaaagca ggtagcaagt
    47461 ctgccggtga tcgaactggt aaatgctatt gatgctcccc aggtgaaaag ccgcgttggt
    47521 gtaccctggt ttcagatggg ctatcaacct gggcgatatc tggtgcaatg ggcgcacggt
    47581 aaaccactga acgtgctgtt gatgcccgga cccgataacg ccgggggcag taaggagatg
    47641 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
    47701 ggtgataacg atattgaaat ccagcgtaac ctgttgcagg agatgctgga gcgccatcca
    47761 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg ggaagggcgt
    47821 aacctgaaaa cgccgcttac cgtggtatcg ttttatcttt cacatcaggt gtatcgcggg
    47881 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
    47941 gcggttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
    48001 ccgattttag ttctgacgcc gaaaaatgcc gaccgcgaac atatccaccg ctcgctgtca
    48061 ccggggggat ttcgtccggt ctattattat cagcacacat cagcggctaa gaaataacct
    48121 tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat ggcgtaaacg acgaattaac
    48181 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgcagcaga
    48241 cgttcacggc tgagaatttc gcccggattc gtcacaaatg ccaccaacat ttcatactct
    48301 gcgcgggtca gtttaattgg ctcgccatct cgctccagcg tatggcgcga cacattcagg
    48361 caataacccg caaagcgata gcagttgtcc tgagtgagtg gttgcgcctg tcgcgcgagg
    48421 tcgattcgcc agagcagatt tttcactcgt accaccagtt cgcgcagttc cagcggtttg
    48481 gtgacataat cgtctgcacc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
    48541 ccggtaacca gaataatccc taccgttgag cgttctcgca gggcgcgagt taacatcagg
    48601 ccattttcat cgggcaaatt gatatccagc agaattaaat ccaccggctg attctgcata
    48661 atttcccgca gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
    48721 taggattgta atcgcgcctg ggtaaccggt tcatcttcaa caataacaat gtgatgtggc
    48781 atcagagagt tttactcatt ctgttcatat ctgttcatat tctgccgtaa gccgttcatc
    48841 ctgaccagtg ccgctgttca tatttactca ttaagatcgc ttcactaaac cataatttta
    48901 caggggctat tatgcggaaa ttctggaacg cgctacgccg acccagtgct cgttggtcgg
    48961 tactggcgct ggtcgctatt gggattgtga ttggcattgc gctgattgta ttgccacacg
    49021 ttgggatcaa agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg
    49081 tgtatgaaga atataaacag tcggtgcatt tccagaacgc ctctggcgtg cgagctgaat
    49141 gccatgactg tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga
    49201 gcaacgatat ctaccagacc tttattgccc actctattga tacacctgaa aaattcgaag
    49261 ccaaacgcgc ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa
    49321 cctgccgctc ctgccataac tacgacgcga tggatcatgc gaagcagcat cctgaagcgg
    49381 cgcgtcagat gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta
    49441 ttgcccacca gttgccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg
    49501 ccagtgctaa tgacagtggc gacacactgt actccattga tattaagccg atttatgcgg
    49561 cgaaaggcga taaagaagcc tctggttctc tgctgcctgc ctcggaagtg aaagtcctta
    49621 aacgtgacgg cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc
    49681 agcgtgttct cacccagttc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg
    49741 tgcagcagca ggtgaaaacg ctggagaaaa ctaccgttgc cgacaccaat actgagtgga
    49801 gcaaattgca ggccactgcg tggatgaaga aaggtgacat ggtgaacgat atcaaaccga
    49861 tctgggctta tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg
    49921 aaatctctca ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta
    49981 ccagcctcga taaacgtgaa gaacgcacct tgttgaaata tctccaaatg aatgcgtctg
    50041 acaccgcagg taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct
    50101 ctttcaggca tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat
    50161 gctggggccg tcattgttaa cgccgcgcag tgcgactgcg gcgcaagcgg cgactgaggc
    50221 tgtcacctcg aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt
    50281 gaaggagggt cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat
    50341 gatcgccgga ttgccggatc acgtacacaa cgcggcgcgt attcgttacc cgatggtacg
    50401 tgtggactgg ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt
    50461 tgtgcgcgtg agttgggatg aagccctcga catgttctat gaagaactgg aacgtgtgca
    50521 gaaaacgcac gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt
    50581 ccataacgct tcggggatgc tggcgaaagc gattgccttg catggtaata gcgttggtac
    50641 gggcggagat tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcgat
    50701 ggaagtgtat gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt
    50761 gctgtggggc tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga
    50821 tgtttatgaa tattacgagc agctaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat
    50881 cagcatcgat ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca
    50941 cattgcggtt aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcgc atacgctgta
    51001 cagtgaaaac ctgtacgaca aaaacttcct cgctaactac tgtgtaggtt ttgagcagtt
    51061 cctgccgtat ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa
    51121 actgaccggc attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag
    51181 aacgcaaatt attgctggct ggtgcgttca gcgtatgcag cacggtgaac agtgggcgtg
    51241 gatgattgtc gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggctttgg
    51301 ttttggctgg cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttctgagtgg
    51361 tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca aaggttacag
    51421 cagcaccatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa
    51481 ctggaacggt aaatcggtaa aactaccgcc gctgaaaatg tgtatttttg ccggaactaa
    51541 cccgttccat cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac
    51601 ggttatcgcc atagataacc aatggacctc aacctgccgc tttgccgata tcgtgctgcc
    51661 tgcgaccacg cagtttgagc gtaacgatct cgaccagtat ggcaaccact ccaaccgtgg
    51721 cattatcgcc atgaaacagg tcgtgccgcc gcagttcgag gcgcgcaacg actttgatat
    51781 tttccgcgag ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga
    51841 aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag gacgcggcgt
    51901 tcatctgcca gcgtttgatg acttctggaa taacaaggag tacgtcgagt ttgaccatcc
    51961 gcagatgttt gttcgtcacc aggcattccg cgaagatccg gatctcgaac cgctgggcac
    52021 gccgagtggc ctgattgaga tctactcgaa aactatcgcc gatatgaact acgacgattg
    52081 tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ctggctcgca
    52141 gaagtatccg ttgcatctgc aatctgtgca tcctgatttc cgacttcact cgcagttatg
    52201 tgagtcggaa acgctgcgtc agcaatatac ggtagcgggc aaagagccag tgttcattaa
    52261 cccgcaggat gccagcgcac gcggtattcg taacggtgat gtggtacgcg tctttaacgc
    52321 tcgcggtcag gtgttggcag gggcagtggt ttctgaccgt tatgcacccg gcgtggcgcg
    52381 aattcatgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg cgctgtgcaa
    52441 atacggtaac cccaacgtgt tgaccatcga catcggtact tctcagctgg cgcaggcgac
    52501 cagtgcgcac actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtaac
    52561 ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg cgtcgcaggt
    52621 gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg gctggcgcag
    52681 ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct
    52741 gaatggtttt cgttgctgaa aagtgaaccg ccgctcactg cggcggtgaa tgagctggaa
    52801 aaccgtattg cagccctgac cgtacgtgac gatgcccgtc tggaactggc cgctgacttt
    52861 tgcggcctgt ttctgatgac cgacaaacaa gctgcgctgc cgtatgcatc ggcctacaaa
    52921 caggacgagc aagagattaa acgcttgtta gttgaggcag ggatggaaac cagcggcaat
    52981 ttcaacgaac cggcagatca tctggcgatc tatctcgagt tgctcagcca tctgcatttt
    53041 tcgctgggag aggggaccgt tcctgcgcga agaatcgaca gtttgcggca aaaaacgctg
    53101 acggcgctgt ggcaatggtt accagagttt gctgcgcgtt gccatcagta tgacagcttt
    53161 ggtttttacg cggcattaag ccagttattg ctggtgttag tggagtgcga ccaccaaaac
    53221 agataacgtc gtttgttggc ctgataagac gcgtttagcg tcgcatcagg cattagggcg
    53281 cagttgccgg atgcgtcatc acatccggca aacacacatc acggatgagc tacaaaccgg
    53341 gaaagccgct ggcgcagcag gcggttttcc tgcttcaggt gcgcaatatc atccattaac
    53401 gtcagcgcca ccgcgatccc cggccagtcc agagcgagtt catgacgcag gcgtaccgca
    53461 cgttgcacca caatggcggc atggtcgtca aatacccagg ttgtttcctg gatctcacgc
    53521 ggttcaacca cccccaaacc gacaatttca ttcaactcct cttcagagat gccggtatgc
    53581 aggcaaaatt cggtaatagt aaaagtcacc gtaacattag ccattatgct ttcccccaat
    53641 ctttacgtgg atcaaaagac gactgggcgt ctgccagttg ctgccatagc gcggcagtgt
    53701 tttcatccgg tttcggcggc atcacgattt tcagtaccgc atacagatcg ccggtctgtt
    53761 ttttgctcac cagacctttg cctttaacgc gcaatcgttg cccggcctga ctgccaggtg
    53821 ggatagtcag caaaatgctt tctttcagtg ttggaacggt gactttagca cccagcgccg
    53881 cttcccacgg gctaaccggc accacaattt ccagatcctg gccgacaata tcgaacagcg
    53941 gatgtggcgc aatatgaatc accagccaca aatcgccatt tggaccgccg ttttcgcccg
    54001 gcgtcccctg gcctttcagg cggatgcgtt gaccattgcc gacgcccgcc gggatcttca
    54061 cattcagcgt tttcgggatt tcctgttcga tcatgccaaa ggcgttataa accggcaggt
    54121 tatagctgat ggtacgctta tgctcagtaa gcgtttcttc gaggaatacc gccacttcga
    54181 tttcaatatc gtggccgcgt gtggcggggc gttgacggct ctggcgggca tgctgaccga
    54241 aaattgacga gaagatatcg tcaaaatctt cggcgttaaa actctgaccg tcgccatgct
    54301 ggaactgacg gctaaattgc ggatcgttgc gatgttgcca catctgatca tactcagcgc
    54361 gacgttgttc atcacttaac acttcccagg cttcagcgac ctctttgaag cgggcttcgg
    54421 catccggttc tttgctgaca tcaggatggt atttacgggc cagtcgacga taggcggtct
    54481 tgattgtctt gagatcgtcc gtcggtttca cgcccatgat ggcgtaataa tcctttaatt
    54541 ccatagcgtt atctcgcgta aatcaactca aattgaagga acccctgtaa ggtaactcct
    54601 ataagtgtag ggtaatcctc aaaatttcat atgccaacac agaatatgtt attgaaatca
    54661 tcgcggagag gaggtcgcct caaggtgggg tgttgaacat attttaaaca ggtgaaaaag
    54721 ggtgagcgat ttttgatagt tgaaacaggc actttaagtt taactggggc gtcattattt
    54781 attaaat
//
LOCUS       DAYPHN010000010        51655 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000010 DAYPHN010000000
VERSION     DAYPHN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.010"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            116..1780
                     /gene="tsr"
                     /locus_tag="ACG0BW_000633"
     CDS             116..1780
                     /gene="tsr"
                     /locus_tag="ACG0BW_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFK7257643.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFLIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            complement(1823..3094)
                     /locus_tag="ACG0BW_000634"
     CDS             complement(1823..3094)
                     /locus_tag="ACG0BW_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFK7257644.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEMVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3091..3564)
                     /locus_tag="ACG0BW_000635"
     CDS             complement(3091..3564)
                     /locus_tag="ACG0BW_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFK7257645.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            complement(3628..4599)
                     /locus_tag="ACG0BW_000636"
     CDS             complement(3628..4599)
                     /locus_tag="ACG0BW_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFK7257646.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            5290..6942
                     /locus_tag="ACG0BW_000637"
     CDS             5290..6942
                     /locus_tag="ACG0BW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFK7257647.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            complement(7113..8018)
                     /locus_tag="ACG0BW_000638"
     CDS             complement(7113..8018)
                     /locus_tag="ACG0BW_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFK7257648.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            8157..9179
                     /gene="lgoD"
                     /locus_tag="ACG0BW_000639"
     CDS             8157..9179
                     /gene="lgoD"
                     /locus_tag="ACG0BW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFK7257649.1"
                     /translation="MSTMKVLICQEIKNLVWEVWERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            complement(9318..11609)
                     /gene="opgB"
                     /locus_tag="ACG0BW_000640"
     CDS             complement(9318..11609)
                     /gene="opgB"
                     /locus_tag="ACG0BW_000640"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFK7257650.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDIMKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWALELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMAHYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(11863..12357)
                     /gene="yjjA"
                     /locus_tag="ACG0BW_000641"
     CDS             complement(11863..12357)
                     /gene="yjjA"
                     /locus_tag="ACG0BW_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFK7257651.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSNGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(12406..13143)
                     /gene="dnaC"
                     /locus_tag="ACG0BW_000642"
     CDS             complement(12406..13143)
                     /gene="dnaC"
                     /locus_tag="ACG0BW_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFK7257652.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(13146..13685)
                     /gene="dnaT"
                     /locus_tag="ACG0BW_000643"
     CDS             complement(13146..13685)
                     /gene="dnaT"
                     /locus_tag="ACG0BW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFK7257653.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     ITPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALRESVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(13793..14266)
                     /gene="yjjB"
                     /locus_tag="ACG0BW_000644"
     CDS             complement(13793..14266)
                     /gene="yjjB"
                     /locus_tag="ACG0BW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFK7257654.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(14257..15027)
                     /gene="yjjP"
                     /locus_tag="ACG0BW_000645"
     CDS             complement(14257..15027)
                     /gene="yjjP"
                     /locus_tag="ACG0BW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFK7257655.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            15647..16372
                     /gene="yjjQ"
                     /locus_tag="ACG0BW_000646"
     CDS             15647..16372
                     /gene="yjjQ"
                     /locus_tag="ACG0BW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7257656.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            16330..17007
                     /gene="bglJ"
                     /locus_tag="ACG0BW_000647"
     CDS             16330..17007
                     /gene="bglJ"
                     /locus_tag="ACG0BW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFK7257657.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(17045..17833)
                     /gene="fhuF"
                     /locus_tag="ACG0BW_000648"
     CDS             complement(17045..17833)
                     /gene="fhuF"
                     /locus_tag="ACG0BW_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFK7257658.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLSTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            17974..18210
                     /gene="yjjZ"
                     /locus_tag="ACG0BW_000649"
     CDS             17974..18210
                     /gene="yjjZ"
                     /locus_tag="ACG0BW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFK7257659.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(18249..18335)
                     /locus_tag="ACG0BW_000650"
     tRNA            complement(18249..18335)
                     /locus_tag="ACG0BW_000650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18299..18301),aa:Leu,seq:cag)
     gene            complement(18370..18456)
                     /locus_tag="ACG0BW_000651"
     tRNA            complement(18370..18456)
                     /locus_tag="ACG0BW_000651"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18420..18422),aa:Leu,seq:cag)
     gene            complement(18485..18571)
                     /locus_tag="ACG0BW_000652"
     tRNA            complement(18485..18571)
                     /locus_tag="ACG0BW_000652"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18535..18537),aa:Leu,seq:cag)
     gene            complement(18839..19870)
                     /gene="rsmC"
                     /locus_tag="ACG0BW_000653"
     CDS             complement(18839..19870)
                     /gene="rsmC"
                     /locus_tag="ACG0BW_000653"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFK7257660.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            19973..20386
                     /gene="holD"
                     /locus_tag="ACG0BW_000654"
     CDS             19973..20386
                     /gene="holD"
                     /locus_tag="ACG0BW_000654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFK7257661.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRNG"
     gene            20355..20801
                     /gene="rimI"
                     /locus_tag="ACG0BW_000655"
     CDS             20355..20801
                     /gene="rimI"
                     /locus_tag="ACG0BW_000655"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFK7257662.1"
                     /translation="MNTISSLETADLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            20816..21493
                     /gene="yjjG"
                     /locus_tag="ACG0BW_000656"
     CDS             20816..21493
                     /gene="yjjG"
                     /locus_tag="ACG0BW_000656"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFK7257663.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            21584..23173
                     /gene="prfC"
                     /locus_tag="ACG0BW_000657"
     CDS             21584..23173
                     /gene="prfC"
                     /locus_tag="ACG0BW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFK7257664.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            23566..24171
                     /gene="osmY"
                     /locus_tag="ACG0BW_000658"
     CDS             23566..24171
                     /gene="osmY"
                     /locus_tag="ACG0BW_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFK7257665.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            24298..24459
                     /gene="ytjA"
                     /locus_tag="ACG0BW_000659"
     CDS             24298..24459
                     /gene="ytjA"
                     /locus_tag="ACG0BW_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFK7257666.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            24581..25654
                     /gene="yjjU"
                     /locus_tag="ACG0BW_000660"
     CDS             24581..25654
                     /gene="yjjU"
                     /locus_tag="ACG0BW_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFK7257667.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            25651..26433
                     /gene="yjjV"
                     /locus_tag="ACG0BW_000661"
     CDS             25651..26433
                     /gene="yjjV"
                     /locus_tag="ACG0BW_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFK7257668.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYTALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(26849..27712)
                     /gene="yjjW"
                     /locus_tag="ACG0BW_000662"
     CDS             complement(26849..27712)
                     /gene="yjjW"
                     /locus_tag="ACG0BW_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFK7257669.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(27684..29234)
                     /gene="yjjI"
                     /locus_tag="ACG0BW_000663"
     CDS             complement(27684..29234)
                     /gene="yjjI"
                     /locus_tag="ACG0BW_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFK7257670.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANKVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            29492..30271
                     /gene="deoC"
                     /locus_tag="ACG0BW_000664"
     CDS             29492..30271
                     /gene="deoC"
                     /locus_tag="ACG0BW_000664"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFK7257671.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30398..31720
                     /gene="deoA"
                     /locus_tag="ACG0BW_000665"
     CDS             30398..31720
                     /gene="deoA"
                     /locus_tag="ACG0BW_000665"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFK7257672.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            31772..32995
                     /gene="deoB"
                     /locus_tag="ACG0BW_000666"
     CDS             31772..32995
                     /gene="deoB"
                     /locus_tag="ACG0BW_000666"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFK7257673.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            33052..33771
                     /gene="deoD"
                     /locus_tag="ACG0BW_000667"
     CDS             33052..33771
                     /gene="deoD"
                     /locus_tag="ACG0BW_000667"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFK7257674.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(33920..34182)
                     /locus_tag="ACG0BW_000668"
                     /pseudo
     CDS             complement(33920..34182)
                     /locus_tag="ACG0BW_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34214..35230)
                     /gene="lplA"
                     /locus_tag="ACG0BW_000669"
     CDS             complement(34214..35230)
                     /gene="lplA"
                     /locus_tag="ACG0BW_000669"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFK7257675.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGEHVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWIA
                     GAVR"
     gene            complement(35258..35902)
                     /gene="ytjB"
                     /locus_tag="ACG0BW_000670"
     CDS             complement(35258..35902)
                     /gene="ytjB"
                     /locus_tag="ACG0BW_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFK7257676.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36008..36976
                     /gene="serB"
                     /locus_tag="ACG0BW_000671"
     CDS             36008..36976
                     /gene="serB"
                     /locus_tag="ACG0BW_000671"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFK7257677.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            37025..38407
                     /gene="radA"
                     /locus_tag="ACG0BW_000672"
     CDS             37025..38407
                     /gene="radA"
                     /locus_tag="ACG0BW_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFK7257678.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            38428..39660
                     /gene="nadR"
                     /locus_tag="ACG0BW_000673"
     CDS             38428..39660
                     /gene="nadR"
                     /locus_tag="ACG0BW_000673"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFK7257679.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            39694..39807
                     /gene="nadS"
                     /locus_tag="ACG0BW_000674"
                     /pseudo
     CDS             39694..39807
                     /gene="nadS"
                     /locus_tag="ACG0BW_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(39968..41635)
                     /gene="ettA"
                     /locus_tag="ACG0BW_000675"
     CDS             complement(39968..41635)
                     /gene="ettA"
                     /locus_tag="ACG0BW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFK7257680.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            41846..43783
                     /gene="sltY"
                     /locus_tag="ACG0BW_000676"
     CDS             41846..43783
                     /gene="sltY"
                     /locus_tag="ACG0BW_000676"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFK7257681.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            43873..44199
                     /gene="trpR"
                     /locus_tag="ACG0BW_000677"
     CDS             43873..44199
                     /gene="trpR"
                     /locus_tag="ACG0BW_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFK7257682.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(44242..44754)
                     /gene="yjjX"
                     /locus_tag="ACG0BW_000678"
     CDS             complement(44242..44754)
                     /gene="yjjX"
                     /locus_tag="ACG0BW_000678"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFK7257683.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            44806..45453
                     /gene="gpmB"
                     /locus_tag="ACG0BW_000679"
     CDS             44806..45453
                     /gene="gpmB"
                     /locus_tag="ACG0BW_000679"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFK7257684.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(45450..46319)
                     /gene="robA"
                     /locus_tag="ACG0BW_000680"
     CDS             complement(45450..46319)
                     /gene="robA"
                     /locus_tag="ACG0BW_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFK7257685.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            46530..47003
                     /gene="creA"
                     /locus_tag="ACG0BW_000681"
     CDS             46530..47003
                     /gene="creA"
                     /locus_tag="ACG0BW_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFK7257686.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            47016..47705
                     /gene="creB"
                     /locus_tag="ACG0BW_000682"
     CDS             47016..47705
                     /gene="creB"
                     /locus_tag="ACG0BW_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFK7257687.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            47705..49129
                     /gene="creC"
                     /locus_tag="ACG0BW_000683"
     CDS             47705..49129
                     /gene="creC"
                     /locus_tag="ACG0BW_000683"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFK7257688.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGACMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENCQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCIMLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            49187..50539
                     /gene="creD"
                     /locus_tag="ACG0BW_000684"
     CDS             49187..50539
                     /gene="creD"
                     /locus_tag="ACG0BW_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFK7257689.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(50599..51315)
                     /gene="arcA"
                     /locus_tag="ACG0BW_000685"
     CDS             complement(50599..51315)
                     /gene="arcA"
                     /locus_tag="ACG0BW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFK7257690.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            51411..51551
                     /gene="yjjY"
                     /locus_tag="ACG0BW_000686"
     CDS             51411..51551
                     /gene="yjjY"
                     /locus_tag="ACG0BW_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFK7257691.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
BASE COUNT        12476 a        12770 c        13799 g        12610 t
ORIGIN      
        1 gctttttaaa ttcagggggt gaataaaatt actcggcgtc atctccgcgg gattttcata
       61 aagtttttcc tttccaggcc gaaaatcttg catcggtcca caggaaagag aaaccatgtt
      121 aaaacgtatc aaaattgtga ccagcctact gctggttttg gccgtttttg gccttttaca
      181 actgacttca ggcggtctgt tctttaatgc cttaaagaac gacaaagaaa atttcactgt
      241 tttacaaacc attcgccagc agcaatccac gctgaatggc agctgggtcg ctttgttgca
      301 gacgcgtaac accctcaacc gcgcgggtat ccgctacatg atggatcaga acaatattgg
      361 tagcggttca accgttgctg agctgatgca gagtgccagt atttcgctga aacaggcgga
      421 aaaaaactgg gcggattacg aagcgttgcc gcgtgacccg cgtcagagca ccgccgcagc
      481 ggcagagatc aaacgtaatt acgatattta tcacaatgcg ctggcggagc tgatacaact
      541 gttaggtgca ggcaaaatca acgagttctt tgatcagccg acccagggat atcaggacgg
      601 tttcgagaag cagtatgtgg cttacatgga gcaaaacgat cggctctatg atatcgccgt
      661 cagcgataac aatgcctcct acagccaggc gatgtggatt ctggtaggcg tgatgatcgt
      721 cgtactggcg gtcatcctcg ccgtctggtt cggtattaaa gcctcgctgg tagcgccaat
      781 gaatcgcctg attgacagca ttcgtcatat tgcaggcggc gatctagtga aaccgattga
      841 ggtggatggc tctaatgaga tggggcaact ggcagagagt ttgcgccata tgcagggaga
      901 gctgatgcgt accgtcggtg atgtgcgcaa cggggccaat gccatctata gcggtgccag
      961 cgaaatcgcc accggcaata acgatctctc ttcgcgcacc gagcaacagg ccgcttcgct
     1021 ggaagagacg gcagccagca tggagcaact gaccgcaacg gtgaaacaga atgccgaaaa
     1081 tgcgcgccag gccagccatc tggcgttaag tgcttccgaa acggcgcaac gcggcggcaa
     1141 agtggtggat aacgtggtgc agaccatgcg cgatatctcc accagttcgc agaaaatcgc
     1201 cgatattatc agcgtaattg acggcattgc cttccagacc aatattctgg ctttgaacgc
     1261 ggcggttgag gctgcgcgtg cgggtgagca aggacgcggt tttgcggtgg tcgcgggaga
     1321 agtgcgtaat ctggcccagc gtagcgctca ggctgctcgt gaaattaaaa gcctgattga
     1381 agactcggtg gggaaagtgg atgttggctc tacgctggtc gaaagcgccg gggaaacaat
     1441 ggcggagatt gtcagtgccg tgacccgcgt gacggacatt atgggcgaaa ttgcttctgc
     1501 ttctgatgag cagagccgtg gtatcgatca ggttggctta gcggttgctg agatggaccg
     1561 ggtaactcaa cagaacgccg cgctggtgga agaatctgcc gccgccgccg ccgcgctgga
     1621 agaacaggcc agtcgcctga ccgaagcagt ggcagtgttc ctgattcagc aacagcagca
     1681 acagcagcgt gaagcatcgg ctgtggtaaa aaccgtgacg ccagctacgc cgcgcaaaat
     1741 ggccgtggca gatagcgagg agaactggga aacgttttaa cgtaatgatg ccggatgagt
     1801 atttcatccg gcgtttttat tattaaacca ggaacagtga aatcgccggg atatatgtca
     1861 ccagcattag cattaacagt agagccgcca gtaggggcag ggattgttta aagaaatttc
     1921 ccaccgaaac ttgcgtgata ttacatacca gcagcatcac ggtacctacc ggtggcgtga
     1981 atgcgccaat cgacaggttg aagatcatga tgatgccaaa atgcaccgga tcgatgccaa
     2041 agtgttcgac caccggtttt aatagtggta ccaggatcag caaaatggcg tttccttcca
     2101 gaaacatccc cagcgtcagc agcaatatgt tgataatcaa caggaacatg tatttgtttt
     2161 cggtcagcga agtcacaaaa ttcgccactg cgacaccagc atgttccatt gagaaaatcc
     2221 atcccaatgc ggcgctggcc atgattacca gcatcaccga tgcggttgcg cgtgccgttt
     2281 cgcccaatgc tttgacgaca tgagaaatcc gcatttcacg atagataaaa aagccaatcg
     2341 ccagcacgat tagcaccgct actgcacctg cttctgttgg ggtgaaaatg ttagcgcgaa
     2401 taccacccac cacagcaaaa atgaggaaca gcgcaggcca ggcctgtccc agcgagaaca
     2461 ccatctcttt gcccgtaggc cagctttcac gcgagggttt agcgttagtt ttgcgggcga
     2521 caaggtagac ggtaaacatc agtacgactg cgcatagcaa accaggtacc agtccggcga
     2581 taaacatttt tccgatagag acatcggcaa ccagaccgta aataatcagg gctattccag
     2641 gcggtatgat cggggtgact aacgagccac cagcggtaat ggcggcggca aacgcgcgat
     2701 tgtagccttt acgttccatc tccggcacca tcatgcgcga cagcatcgcc gcgtcagcaa
     2761 ggttagaggc gctgacgccg cccatcatgg tgctgacgag aatgtttgcc acgcccagtc
     2821 cgccgcgcat tttccccacc agaacgttgg caatgtgcag gatgcgttcg gcaatgccgg
     2881 tgtagctcat tagtgtgccg agcaagataa aaaacggaat tgccagcaat gatgggcttt
     2941 gcgttggcgc gattagtctt tgcacggcaa tttcaatcgg tacgctggag aagaataaaa
     3001 agtaagcgat gatgccaata aacatcgcca gatagacccg aatgttgaga aacaaacaga
     3061 cgatcattag cggaataatc aataaccaac tcattgcatt ttgtctccgc tatcggattg
     3121 ttttgtatcg tttattaacc agtagagggt ataaatagcc gttgccagtg cgccgacagg
     3181 gaccgccaga tcgatccaga accaggaaac tttcagaatt tgtgtcactt taaactgcgc
     3241 catttcggct agtcggtagc ccagccaggc catataaagc agcagtgcga tggagagaag
     3301 ggcgaccagt actgcaataa cacgcctgag tttgcctgga agcatctcca tcaggaaggg
     3361 aatggtcagg tgcgccctgt cacgttccgc caccactcca cctaacatca ccacccaaat
     3421 catcagcatt cccgacattt cttctgtcca ttgaagcggt tggccgatga aataacgcat
     3481 aaataccgct ccaattgtca ccacaaccag aacgaatagc gcaaaggcag cactaaattc
     3541 tgccactttg ccaaaaagac gcatgaaacc tcctgttagt gcgtgtcctg cctaagagag
     3601 atgaggcagg acgctgagca ttaatgctta ttgcagttgc tgttgaatgg tttcgtacag
     3661 acctggtgtc cattccggga attgagagta aacttcgcgt gctttcttct ggaatggtcc
     3721 agtatcggga tagatcactt cgacgccggc tgcctgcatg gttttcaaca tttcggcatc
     3781 acgctcaaga gtgagtttct ggctatagac acccgcttca tagccggtct gatggatcat
     3841 ctccagttgt tctggtgaaa gggtgctaaa gaaggcttca ccacctatcc acactgaggt
     3901 atttgtcagg tagttaacca ttgtcagata tcttgcctgc tcatacagtt tttgcccttg
     3961 cagcactgaa attgggtttt ctacgccgtc aataacgccc tgggttaatg cgggataaac
     4021 ttcgcctaat ggcatgggag ttggtgttgc ccccatcgcc tgaatggctt ttatttgcat
     4081 gacgttatta ggaacgcgaa tttttaaacc tgcgagatcg tcaacggtgc gaatgggttt
     4141 tttcgaaata atctgtcgag tgccatagag ataattattc attaccacat gaattccctt
     4201 tttcttcaga ctttcatttt tttgtttaaa ccagtcgctt tcatatattt tgaacagttt
     4261 ttgcggatcg tcagtcagat acggtccaaa caaaatcccc aaatcgggtt cataatcggc
     4321 aagaaaagcc acatcactca aggttatgac attcatgccc atcatggctt gctcggtgac
     4381 atcctgtttt gatcccaatt gggagctggg atagagcgca agtgttattt cgccattact
     4441 ttttttattt aataggtctg cccagtaacg catgaccaca tccaggggtt ctccagggtt
     4501 attttcatag gcaactttta ttgaaatagg ttttgcctgg attattgacg gtaaaaaaag
     4561 ggtactgaca gtaattagca tcgctattat tttgctcatc tttacttact ccatgctaag
     4621 gaaataaaac tccaacattg ttgtcgggag tgaaacctta tgaaaaagca gtcattactg
     4681 aaaagtagtc agcgaatagc agaatggctg cagatgtatc ataaattcgt gcaccgggta
     4741 atactttgct gcttttaatc ggtttttaat gcattttttg attgtatatc ttaaagttat
     4801 gtaatgcatg acgttacaag gaacaccctc tgatgagttt agttaccgct gataagatct
     4861 cttattctgt tgatgattta ttaaaaataa aatcattaat ttggctgcgg ccattttaaa
     4921 ttgtcaatac agataaaaac taagtgttga cgcagaatgg tggtatccat taaatttatt
     4981 tagtgctgat ttaatacatt tgctgaacgt ataattcatg ctgatattaa aagaaaaccg
     5041 tcattcacta aataacttgt tcggcaagga aatcagagag taacggatga ttcgttacaa
     5101 cggggaaagg cagtaaatat tcacgtggtg aatttgttat caacttgaaa actcatttta
     5161 ctgggttcct tctgatcatg tgagtgaacg cagtgattaa tgattaacgt taagtgctaa
     5221 ccagagcgct ttttatgaag ttaaattaat aatatccatt atttatgaat gaggaagcct
     5281 gtttattata tggaaatact tttaacaatt ttaattctta cgttggtagt atcattaacc
     5341 agcgtgctta cacgtctgtc tcctgtccag ataccacttc ctttaatcca gattgccgct
     5401 ggcgcagtac tggcccagcc tatatttggt ctgcatgtgg aattcaatcc tgaacttttt
     5461 ctcttattgt ttataccacc cctgttgttt gctgaaagtt caaaaataca acctaaagaa
     5521 ttgatcaagc acagccggga aattatcagc ctggcacttg tgttggtact aatcactatt
     5581 tttggtgtcg gctatgtgat tcatttactc ttaccgaatg tgccgctcat cgctgctttc
     5641 gcattagccg ccgtgttgtc gcctaccgat gccgtggcgt tgctggggat tgtcgggaaa
     5701 gggcgtatca gcaaaaacat ccaggaagtg ctcgaaggcg aagcgttgat gaatgatgct
     5761 tccggcctgg tggcgttgaa atttgctgtt gcagtgacga tgggaacgat ggaattcagc
     5821 gttcatggcg caaccattgc gttttttgtc gttgcgttgg gcggtattgc cgtggggatc
     5881 gctgttacct ggttatatgg taaagggctg ctgttgatca gccgatatgc tcacgatgaa
     5941 ccttccattc agattgtatt gatgcttctg ttgccgttta ttgtttatct ggttgcggaa
     6001 cattttggct tatctggcat tcttgccgct gtcgccgcgg ggttaacaac cagtcgcatt
     6061 ggtgtagtcc accacgcgcc tattcgcctg agaattaaag ctaaaagtag ctggtcgatg
     6121 cttgaatatg tctttaacgg catggtgttt ctgattttag gcttacaact gccgggcatt
     6181 atttcggaat cactgatccg tgcagaaaac gatccactgg tggacaccgg atcgctgctc
     6241 cttgatgtca tttggatcta cctggcgtta atggttgtgc gcctgggctg gctttggctg
     6301 atgcgtctgt atagccgtaa acgacccggc aaacatccga tgctgtttgc tggatataaa
     6361 acgcgtcata tgctgctggc gacctttgcg ggggtgcgcg gagctatcac gcttgcgggg
     6421 gtgctttcta tcccgttatt gctgccggat ggcacacctt ttcccgggcg ttacttgctg
     6481 gtatttctcg ccacaggtgt gatcctcttt tcgctggttt gcggcattat tattctgccg
     6541 ttattactgg ggaaaaatgt ggggattttc agcgataacc gacaaaaaga agaggaagct
     6601 gccgatctgt ttatggctaa aatggccata ctgagtgtgc aaaagaccga gcaacgtttg
     6661 ctgcgcgcca gaacagaaaa cctcgataac gagttgatta tggaaacggg ggccagagtt
     6721 atcggttata tgcgacatag tgaagaggat atgtcgccta atgataattc cctgcatatg
     6781 cagcgtgcga agctggaaag aacatttaat ctggcggcgt taaatgccaa acgcgcggct
     6841 atttaccaga tgcgcgcaag gcaagaagtc agtgatgaaa cactggcaaa aatagtcagt
     6901 gaattagatg tgctggaaac gttaatcatg gatagccgat aattttatcg ctgccagtaa
     6961 tagggaatta aatgatggct gagagtaata cttccagcat cgtttattcc ctatgtattt
     7021 ccttatgggc agaacaatcg cctggattaa tactgcatta atacaacata gtgatataaa
     7081 ccaagaatca atgtttttaa tctgtactta atttatcggt gatgttgcct gatggaacga
     7141 atcattgatt gtttggcggt atccagatgg tttcgcaacg ccgttattgc atctaaatcg
     7201 ctacggcaaa tcagggcgct gagaatggtc atatgttcat caacagcaat gatattgcgc
     7261 tgtttgagat cgctttcatc ccattgatag tgaaaatgaa agatcaccga tataatttca
     7321 agtgattgat taaaaaagat gttgtcagca gcggataaca gcatggcatg aaaatcacgg
     7381 tctaattgag aaaacattcg aaagttgctg ccaatgctgt cgcgcaatgt cctgtgacgt
     7441 tcgagcatgg ttttcgcctg caaccagcga gggtcatcat cgggaaggtt gagaaagtgt
     7501 tgcagggaat gtgtttcgag catttcgcgt agctcaaaca gctgctcggc gtaggattga
     7561 tcgaactgct tcatgctcca ttggccgcgc ttttcgcttt gaataagatt ataacgaccg
     7621 aatttcaaaa gatattctcg taccactacc ggactgacgc ttgccgctcg cgccagctga
     7681 agttcggaaa agctttctcc ggcgcgtaac tggcgcaggt taatcatggt gaaaaatgcc
     7741 tgctcgaaaa gacggttttg ctctttcagc gaggccgtga tgcaatcaaa tccatcgttc
     7801 tgttcgggtt tgcgggcgat aacataattt ttccccacca gggtgagcac gccacatgcg
     7861 cttaaatgct ggagtatgtg gcgtaccgta gtgcgactga tgttatacat ttctgccagt
     7921 ccgctttgtg aaggcagcgg agaaggaata ttgcccctcg ccatatcttc aatcacctga
     7981 tttatgacgc tatgacgtaa attctgcgaa cgactcattc ttccctccag tccggcatta
     8041 aaagccgatt taaaacattt ttatgcgcga tcatccgcat attttatggg taaattcagg
     8101 cgctcttata aattcgacga tatctgaaag tcataaaata aaaggagcgg tgaatcatgt
     8161 ccacaatgaa agtattaatt tgtcaggaaa taaagaattt agtatgggaa gtctgggaaa
     8221 gaccggtgcc aggcgataat gaagcattaa ttaaaattaa aactgtcggg atttgcggta
     8281 cagatattca tgcctgggcg ggcaatcagc cattttttag ttatccacgc gttttaggcc
     8341 atgaaatatg tggggagatt attgaatcag gaaaaaacat tcacaatttt aatatcggac
     8401 agcaagttgc ggtaattccg tatatttctt gtcagcaatg cccggcgtgt aaaagcggtc
     8461 gtaccaattg ctgtgaaaaa atttcagtca ttggcgtgca tcaggatggc ggttttagtg
     8521 agtatttgag cgtgccggtg gcgaacattt tgcccgcaga cggtattgac ccgcaggcgg
     8581 cagcattgat tgaacctttc gctattagcg ctcatgcggt gcgtcgtgcc gccattgcac
     8641 ccagcgagca ggtgctggtg gtcggggcgg ggccaatcgg tctgggcgcg gcggcaatcg
     8701 ctaaagccga tggcgcgcag gtggtggtgg cggataccag tccggcgcgt cgtgaacatg
     8761 tggcaacgcg tctggaatta cctgttctgg acccgtcagc cgaagatttt gacgcgcagc
     8821 tgcgggcgca gtttggtggt tcgctggcgc agaaagtgat tgacgcgaca ggtaatcaac
     8881 atgcgatgaa taacaccgta aatctgattc gtcacggcgg cacggtggta tttgtcggcc
     8941 tgtttaaagg tgagttgcag ttctccgatc cggaattcca taaaaaagaa acgacgatga
     9001 tgggcagccg caacgccacg ccggaagatt tcgctaaagt cggtcgattg atggcggaag
     9061 ggaaaatcac tgccgacatg atgttaaccc atcgctatcc gttcgccacg ctggcagaaa
     9121 cctacgagcg cgatgtgatt aacaatcgtg agttgattaa gggtgtaatt actttctgat
     9181 aaggcgagat tattaaagtt gccatgcagc gtccggggaa gtgttgggcg ctgttttttt
     9241 ctgtttctca atgatgtttt gtaggccgga gaaggtgtgt aggtcgcacc ttatgcgacc
     9301 tacacatcag tcaggaatta cccttcacgt tccacaactt taatttccac catgccgatc
     9361 ccgagcttac gcggcgagtg tccgaggata ttcccttcgt tggttgagac aggttccggc
     9421 ggcacaatta ccagtgtgtc ggcatcggtt gggttatcga aatgcagtgt ggtggtggtt
     9481 acttcattgc ccagcacaag agtttgttct tcattgccta cgcgtaccgg aataggacgg
     9541 ctggcgttat tgccgtatgc tttggcggta atcaccaggt caaatttctt cggcagcgga
     9601 tgcttgtact cgatttttac ttcatcgccc agctgagcgt tggaccagcg gccccacgac
     9661 tccggacggg aaatcccgct aaactgtttc acctcttctg gcgcaccggc gacgttgaag
     9721 atgaaactgt cggctttgta gcgaatatcg ttatcaacaa ttttcagcgt atcgacattg
     9781 cttttgtaat gcgccatgtc gatgaccgta tctttaaatg ccgttttgcc cttccacatt
     9841 gttttatcaa catgctggac aatttgctga ccgccaagct gcccttgcga gacacaccag
     9901 tcggtggaga gtgccagttc cagagcccag agttgtgcca tcttgtagca acggtcaacc
     9961 cagacgaaat tgtcgcgtgg agcgaaatct gccagctgga aacgcagcgg tgctgagtat
    10021 tcgctttccg gcagcggttc cacgcgtttg tctgaaaccc gcaacagcag cggcaaacgg
    10081 aaatggctac ctgagaaggc aatcatgttt ttctgctggt cgatggtgaa ctctttcatc
    10141 tctttaggga atttccacag gcggatgata tccggcttcc acgccaatgt tttctctttg
    10201 atattgagga agatttccga catcgactgc ccggataaac tgctacggcc aagtccgaga
    10261 tagttatcgc caccgagaat gtccagcacc gtcgcgccgt tatccatcgt attacgcttc
    10321 actgccagcg tctcttgctg cggcttgtcg ccacggatga caaaaaacag gttattgcga
    10381 tcctgcttat taaggtattt ccacgccgta ttgttcatcg ctaaatggtc agaagagacg
    10441 acgatgacgg tatctttaaa ccacggtgac gctttgattt tgttgataaa cgtcgcaata
    10501 ttctcctggc tgcaacttac cgcgctgaat gactgattcg gcttaccgtc aaaatcatat
    10561 tttttgcggt tacaggtgcg cgagataaaa ccatccgggt gatgggtatc gactgtcagg
    10621 gtaaacagtg agaatcgctg acctgagcgg gaaagctctt caaatttttt ccacgcttca
    10681 tcgagaacgg tatcgtcgta gaatccccag tcgttgcgat agtgcgggtc ggccaccacg
    10741 cttttcagct cttctgagcc gtataagtgg tcgaagccgt gcgatttcag gaacacatct
    10801 ttaccggcaa aacgcagatt cgcgccctgc acgaaatagt tctgataacc cgagtttttc
    10861 ataatatcgc ccagacagat gttctgcggg aagaagctgg aaacagaagc ggaggcgttg
    10921 ccttcaaatg gggcaaacag cggtatgccg cactgagaag ccaccatgcc cgcaatcgtg
    10981 taatccgttc ctggcagctg ctgcgtgtgg ctgaaatcca ggccttcatt tttcaacgcg
    11041 cccagttcag gcgtgagatc cgggaaagcc tcgttatcaa aataggtccg ctcgagactt
    11101 tcgccgtaga tataaaccag gttgagtttc gggtcaggga tagttttcga cggctcttta
    11161 taataagccg caaagtccgg gtcgccgtcg cgtgactggg atttcaccag ttccgttatc
    11221 tgacgaaatg ccgggctggc atccactgaa cccagcgcca gtaagagtgc cagcaggctg
    11281 taaccaaaat ggtgcggatg atgacgacga cggcgcagga tccagcccag cgcaccgaac
    11341 accgccgcca gccccagcac aatgccgata ccaggcagga tgtatttgct gacaccagca
    11401 ccggtcaggc tgttggttaa ggtatagaga accgcgtcgt taataccatc gccagtaaaa
    11461 tagtcgctgg caaacagggt gatatttaaa acgacaaata gccccagtac cgttaatgtg
    11521 gctgcaaacc accaggtgtt acgtcccgct ttccatgcgt aaatcagcac agaggcgaga
    11581 aaaagggcga aagagagtag ttctgacaac gggcgatcct cactaaaccg ggccgtttag
    11641 ccaacgttta agctttttga gaagcacaat gtaatggcgt cgccatattg ctgcaattct
    11701 agtgtcaaaa aaatgcgatg ttgttaggaa ttagtttata aacagacttt tttgatcgcg
    11761 gtcgcttccc gtgtaacagg ggtaaaaccg tccaggagca gggaaaaggg caggagagag
    11821 ccgcgtcagc cgggtaacag ctaaaacgcg gcgtttcgcg gttcaggaaa tgaagttcag
    11881 cccctgcttt aacaccagat cgcaggcttt ggttttcacc ttttctgcca gcggagtcgt
    11941 tccaatatta tccagattga gttgctgacc atctttggtt ttcagcaaac cctgaatgcc
    12001 atccagatag ttggtgtctt ctttttgctc ttcactgttc aggcccagtt tttccagtac
    12061 ctgattcttg atgttttcgg catcggttac cgaagccagc ttttgcttcg cgcagtattg
    12121 cagaatgcct gcggcgttgt tcatgttatc tgcgcttaag gcctggttcc cgttgctaag
    12181 caagttagtt aatgacgcga gcgaccaacc gccttcctgt gtcgtgttgt tttggttgcc
    12241 aagttcgctg gcggcgctgg agagcgcatc tttccaggac gcagcatgca ccccggtgga
    12301 aattaatgcg ctggcggcaa tggcacagca cagaagatgt ttaatagttt tcatcatcat
    12361 tactcaaggt tgaattgtgt cgcagtatac cgccataaac ggggattaat actctttacc
    12421 tgttacacgg ctacggtagc tatcccagtt gaagatcacc cacaaactgt tacccaggcg
    12481 catacggtcc atcacgcgtt cgcccagcaa cttggtcatc tcttccatat tactgttggt
    12541 cagcatcccg gttgggcgtt tggaagaaga gcggcgatcg acgatctggt tgatgatcac
    12601 tttttcgtat ttcgattcgg tctgcacgcc gatctcatcg atcaccagca gatcaacgtt
    12661 actcagatcg ttaagcagtt gttcttcgct ggtaccgcta ttcctgaagg tatctttcat
    12721 cgccgacata atatcggcca cggtgatgat caatacggat ttaccgcgta gcagcagctc
    12781 gttgcagatt gccgccgcca gatggttttt gccggttcct ggcttgccag aaaagatgaa
    12841 gctggcgatg ttgccgtcaa actcttcgac atactggcgc gctttgctta acgcattcat
    12901 ttgcccttca cactcaacgc gatagttctc aaaggagcag ttctgatgca gtggacgaat
    12961 accggagcgg ttaaaggtgc gctgcatttt catcgcccga ttttcacgtt cgagagcggc
    13021 ggagcggatc gccccttgtt ctttctgcca ggccagtaat tcttcacccg ttttgaaagc
    13081 aggtttgata tgggcaggca tcattttttg caggcgttgc atcaggtcgc caacgttttt
    13141 catcgttacc ctctgaatcc tggtggaatt tggctgtcag gttcactgac cgtattcaca
    13201 tctcgtttcg gcagtccgcc gttgctggca cgaccgattt gcaggctgcg cgccagtttt
    13261 tgttgccact gcacatggtg aaagactttg ccttccgcct gccagtaggc aatgaatgac
    13321 gccagttctt cggcggtcac cgactctctt agtgccacgc cccatagcgc cgccaggcgg
    13381 ataaagtcgg catcgggttg ccagtctggg tacatggcaa atttccccat gggtacggca
    13441 acgggagcgg tttgcggttc ctgatagagt tgatcgtcca gagcgacatc gcttcccgga
    13501 cgcgccagct tttcttccag cgccagcagt tcagccaggc gtgcaggagt gatggcataa
    13561 aacgccgggg cattgttagc aaatacggca accacaccgc cttcagcttt tgccagaacg
    13621 gtttggtgat cgtgtaccag ggcgtcaata ccaacgacgt ccggggtcaa aactctggaa
    13681 gacatactgt ttctcaaata tggaacgatg aacgagaagg gagataactt ttatagtaac
    13741 acacggagga gaggaggctg tctctcatcc gcgtgcttta agtgaggcaa ttttatacgc
    13801 gagggcgctt gcggtacaac cataatccag gaatggaaag accgatggat aacgcaccaa
    13861 caatcgatga agctgtaaga aagttggtta acagggtaat catcaacggt tcgctgtaac
    13921 ctagctggct gatttttacc gccgaaatca ttgcggtata agctgatatg ccagggaaca
    13981 tagggataac agccgccacg gtaaacactt tcggatgcgc cagataccag cgcgaccatt
    14041 gaataccaat ggtgccgacc agcatagagg ccataaaggt tgaccattca atattcagcc
    14101 cgctggtcat taagatcatt cgggaaccat gacctatcgc accaagcagc gcacaccagc
    14161 gtaacgctcg aacgggaacg ttgaacacca tcgcaaagcc gaccgcagga atggcggcga
    14221 ggatcatatc ctgcgccaac gctaacagaa attcgatcac acccatccgc gtagccccca
    14281 aatcgtcagt gccattacta cgccgacaca ggtagccagt gtcagcagac tggcgattgc
    14341 ccagcgtgcc agtccggtat tgatgtggcc tttaaacata tcggcgacgg catttatcaa
    14401 cggaaagccc ggcaccagca gcagaacgct ggcggccatc gcaatggtgg gggtattgct
    14461 gaaagtggga agttgcaaaa gcaatccgga aatggtggtg gcggcgaaag cggtgaggca
    14521 aaagttgatc tgtggatgaa gatgacgttg tgccagcagc tggcggatat acatcgcggc
    14581 cgtactggcc aggaaggtga tgacggcacc atcccagcca cctttattga gtttacagaa
    14641 acaggcgcag gaaaggccaa ccattaaggc taccagccat cttgggtaac gtaatggctg
    14701 aatttggcta aatcgtttct ctacgccttt gtaatccagc agatgatgct ccgccagaat
    14761 tacaatgtgc tggacttcag tcaccacatg catattaatg ccgcgatcgt gatttttacg
    14821 tgtcgatgtc aggcattgcc catctttaat ggttgtcagc actatggcgt tcgaagagat
    14881 agaactttcg acgctgtcca ttcccaacgc ccgacccagt cgtgaggaaa gctcatcaac
    14941 caacgcgctt tccgcaccat gttgcaaaag aaataatcca cactggatac aaagccgtgt
    15001 tacggctcgc tgttgctcag tttgcataac tcgccctttg tcatagacgt gctgcgtatt
    15061 gttcagataa tgagaatcca tttgtatcat gaagcacggt tgttttgcgg taggttaaga
    15121 tcaaaattgc gctgattaat cctggttatc gggagatgct cggtgataaa aagcgaatta
    15181 tctctcgccg taaacaaact atcgcatgaa tattatcact ataatgaatt tgttatttgg
    15241 cctttacgag tcaggataat agatagctgg gcgcgttttt ttatgttaac aagttaaaac
    15301 catcagattt tattgaataa atattgattg tgtgattccc gcactctgcg ggaagggata
    15361 tcatttaatt ataaattaaa tgaatgtgat ttctgttttt cattaagtat tcctaacaac
    15421 ttaatgtaga tattttaaat gtctccaggc tatttcacca ggttatcatt tgatgctaac
    15481 cttctgacac atgcagtgga gttgttgtgc agcaggagta tgctgatatg aaagtgaatg
    15541 ctaaggataa tttattcgct taatctatta atttgctgga aacatttaag gaatgaatat
    15601 cctgaataaa ccatcatgat ttttagacag tggatgtgga ggaaatatgt tgccaggatg
    15661 ctgcaaaaat ggaattgtta tcagtaaaat acctgttatg caagcagggt taaaagaggt
    15721 catgaggact cacttccctg aatatgaaat aatatccagc gcctctgcgg aggaccttac
    15781 cttattacaa ttacgtcgtt ccggattagt cattgctgat ttagccggtg aaagtgaaga
    15841 tccacgttct gtttgtgaac attattattc tttaatctca caatatcggg aaattcactg
    15901 ggttttcatg gtgtcacgct cctggtattc ccaggcagta gaattgctca tgtgccctac
    15961 ggcgacgtta ttgtctgatg ttgaacccat tgagaatctg gtcaagaccg tacgttccgg
    16021 caatacgcac gcagagcgta tcagcgccat gctgacctct ccggcaatga ctgaaactca
    16081 tgattttagc tatcgctccg tcattctcac tctttcagag cgcaaggtac tgcggctatt
    16141 aggtaaagga tggggcatca accagatagc ttcgttgctt aagaaaagta ataaaactat
    16201 cagcgcccaa aaaaacagtg cgatgcgtcg actggcaatt cacagcaacg ctgaaatgta
    16261 tgcatggata aatagcgcgc agggtgcaag agaacttaac ttgccttctg tttatggaga
    16321 tgccgcagaa tggaacacag ccgaattaag aagagaaatg tcgcactcat agaaaaatgc
    16381 gtcatgagca gtatcggtat tgagagttta ttcagaaagt ttgcgggtaa cccttataag
    16441 ctccatacct ataccagtca ggagtcattt caggatgcca tgtcgcggat ctcatttgcg
    16501 gcggtcattt tttctttttc tgccatgaga agtgagcgca gagagggatt atcttgcctg
    16561 actgaactgg cgattaagtt tccgcgtacc cggcgtttag ttattgcgga tgatgatatt
    16621 gaagctcggc tgattggttc attgtcgcca tcaccgctgg acggtgtatt aagtaaagcg
    16681 tcaacgctgg agatttttca tcaggaactc tttttgtcat taaatggtgt acgtcaggcg
    16741 accgaccgac tgaacaatca gtggtacatt aaccaaagcc ggacgttaag cccgacggag
    16801 agagaaatat tgcgctttat gtcgcgtggc tactcaatga cacaaattgc cgagcagctt
    16861 aaacgcaata tcaaaacgat ccgtgcacat aaatttaatg tgatgtcgaa actgggcgtc
    16921 agttctgatg cagggttgtt ggaggccgca gatattctgt tatgtatgcg gcattgcgaa
    16981 gcaagtaatg tgttgcatcc ctattaatcc gcattatgcc gggtttactt ccccggcagt
    17041 gctttcattt cagcgtacaa tcgccacatt gctgcacatc cggtaagcga taacgctggc
    17101 agcaagtgcg gcgcaccagc aggccgtcgc gcagtaccac ggtacgccag agtggattat
    17161 cttcaccggt agaaagcgtt ttctcaaaaa agagggcatg gcgcagcgat tcaaccgtcg
    17221 cctcgccgag cagttgcttc atctcagtga gataccagtt gatcaaataa ccggtattac
    17281 tccagataag tttgccgttg atctctccgg tcgcttctag tgcctgcaca accggaacca
    17341 gcgcctggct gattaacgtt tccattcgct gctgcggcga atgtggtgtt gcgtttttat
    17401 cttcacacac atcgacccag aaacaggcgg cacggccggt ttcgtgaaac tcagcatgga
    17461 aatgttccgg cgacacatct aatgcctttt cctgcgtcag tagcgccagc attaatggtg
    17521 gcaccatcag gccgatatac cattgtgccc atagcgagat cagcggtttg ttctcgcgga
    17581 tcatcgttgg ttggttgcga tagatatgat cggaatagac cgcgagtaga gaacttagcg
    17641 cattcggtga tgaccattgc gccagcgtca tggcgttaag cggagcgggt tcatccaggc
    17701 ggataaactc cagcaaatgt tcacgatgtt cggcgatcgt cgcacgtacg gcttgtgcaa
    17761 gcccagcatc ctgtggctgg agatgcgttc gccagataac atcttcatag agcggtgcgg
    17821 aacgataggc cataatcggg atagtaatct aaatgataat gattgctaat cctagcgata
    17881 ggtttatccg atagcaaggg atttctctgg cttgcaaatg ataaaaatta tcatatgata
    17941 ttggttatca ttatcaatga aagagatgaa accatgttgc aacgtacgct gggcagtggc
    18001 tggggagtgt tgctgccagg attgctgatt gcagggctga tgtatgcgga tttatcaccg
    18061 gatcagtggc ggattgtcat tctgatggga ttagtattga cgtcggtgat gctgtatcac
    18121 aaacagttac ggcattacgt tttgctacca tcgtgcctgg cgcttattgc tgccatcatg
    18181 ctgatgataa tgaatttgaa tcagggatga aaaatcaagg aagaaacaag aaaggaagta
    18241 aagataattg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
    18301 gaagctagcg cgtctaccaa ttccgccacc ttcgcacagt catcttactt tttttgatat
    18361 cgcctcgttt ggtgcgaggg gggggacttg aacccccacg tccgtaagaa cactaacacc
    18421 tgaagctagc gcgtctacca attccgccac cttcgcccag tgcgagcaat atcaacgtgg
    18481 tttttggtgc gagggggggg acttgaaccc ccacgtccct aaggacacta acacctgaag
    18541 ctagcgcgtc taccaattcc gccaccttcg cataccatca attcttaaaa agaattgcta
    18601 ccacggaggc gcattctagt ggttttcagc ttttcgtcaa tagttaatta tcgccggagg
    18661 tgtaattgct ggaaaaatgt ccatcaggaa actagcgtgc aggtttggta ggcatgcggg
    18721 ggcagatgcc agatgcgacg ctggcgcgtc ttatctggcc tacgaagggc taacgtgcag
    18781 gttttgtagg tcggataagg cgttcacgcc gcatccgaca cggtattcgg cgagataatt
    18841 aacctttctt cgcctggcgg gtcataatgg cgcgatacac cttgaaacgc ccggtttgcg
    18901 caatcacttc atggaagcca aatgtctcat ccagcacgtc cgggtaaggc aggaaggcgt
    18961 tcgctacaat tcgcagctcg ccgccgctat taagatgacg caccgcgccg cgaatcagcg
    19021 tttgcgccgc atccaggctg gtttgcatcc catcgtggaa cggcgggttg gagatgatca
    19081 tatcaaaacg acctttcacc tcggaaaaga cgttgctggc aaagacttca ccttcaacac
    19141 agttggccgc aagtgttgcg cggctggctt ctaccgctgg cgcagagaca tcgcacaagg
    19201 tgagacgaat tttcggcgaa tggcgcgcaa aggcaactga aagcaccccc gcgccacagc
    19261 cgacatccag cactttacct ttcgtgtgcg gggttaacgt cgagagtaac aactggctac
    19321 cgacatccag accgtcgcgg ctaaacacgc caggcagcgt tttgaccgtc aggccatcga
    19381 cgctgtattc gccccagaat ttatccgcat caaatactgg ctgtttttcc agacgaccaa
    19441 aatagaggcc acagcgacga gcgctgtcga ctttattcaa cggcgcataa tctgccagca
    19501 tctgctcggc gctgcgcacg ccgctgcggt tctcgccaac gacaaaaata tccgtcccca
    19561 ccggcagcag agaaagtaaa ttcatcaact ggaactgggc ttccggtttg ttcttcggcc
    19621 agtagtaaat cagcgtatcg caatctgcga cgtcatccgc cgtggcgacc agactgaaac
    19681 gggcgttatc ccccatctgg cggcttaata cctgccagtg gtggaattgc tgggtatgag
    19741 cacggctggc cgcggtatct aaacgcgcgg gcaggtcatc ctgtaagtct ccggcaaaca
    19801 gaatacggct ttgttcgaaa tcatcactgt gacgcagcaa gacttcactt gccggggtaa
    19861 atgcagacat ggaatgctcc tcaattgata ctggcggcga ttatagccat atgttggcgc
    19921 ggtatcgacg aatttgctat atttgcgccc ctgacaacag gagcgattcg ctatgacatc
    19981 ccgacgagac tggcagttac agcaactggg cattacccag tggtcgctgc gtcgccctgg
    20041 cgcgttgcag ggggagattg ccattgcgat cccggcacac gtccgtctgg tgatggtggc
    20101 aaacgatctt cccgccctga ctgatccttt agtgagcgat gttctgcgcg cattaaccgt
    20161 cagccccgat caagtgctgc aactgacgcc agaaaaaatc gcgatgctgc cgcaaggcag
    20221 tcgctgcaac agctggcggt tgggtactga cgaaccgcta tcattggaag gcgctcaggt
    20281 ggcatcaccg gcgctcaccg aattacgggc aaacccaacg gcacgcgccg cgttatggca
    20341 acaaatttgc acatatgaac acgatttctt ccctcgaaac ggctgattta ccagcggctt
    20401 accatattga acaacgcgcc cacgcctttc cgtggagtga aaaaaccttt gccagcaacc
    20461 agggcgagcg ttatctcaac tttcagttaa cgcaaaacgg caaaatggcg gcgtttgcga
    20521 ttacgcaagt ggtgctggat gaagctacat tgttcaatat tgcggtcgat cctgactatc
    20581 agcgtcaggg attgggaagg gcgctgctgg aacatctgat cgacgaactg gaaaaacgcg
    20641 gcgtggcgac actatggctg gaagtccgtg cttcaaacgc tgccgccatt gccctgtacg
    20701 aaagtttagg ctttaacgag gcgacgattc gccgcaatta ctaccccacc acggacggtc
    20761 gcgaagacgc catcatcatg gcgttgccaa tcagtatgta agacaaggtg gaataatgaa
    20821 gtgggactgg attttctttg atgccgatga aacgctgttt acctttgact cattcaccgg
    20881 cctgcagcgg atgtttcttg attacagcgt cacctttacc gctgaagatt ttcaggacta
    20941 tcaggccgtt aacaaaccac tgtgggtgga ttatcaaaac ggcgcgatca cttcattaca
    21001 gcttcagcac gggcgtttcg agagctgggc cgaacggctg aaagttgaag caggcttgct
    21061 taacgatgcc tttattaatg cgatggcgga aatctgcacg ccgctgccgg gcgcggtttc
    21121 tctgcttaac gccattcgtg gcaacgccaa aatcggcatc atcaccaacg gctttagcgc
    21181 cttgcagcaa gtgcgtctgg aacgcacggg cctgcgtgat tacttcgatt tgctggtgat
    21241 ttccgaagaa gttggcgttg ccaaaccgaa taagaaaatt ttcgattatg cgctggaaca
    21301 ggcgggcaat cctgaccgtt cacgcgtgct gatggttggc gacactgccg agtccgatat
    21361 tctcggtggc atcaacgccg ggcttgcgac ctgctggctg aatgcgcacc atcgcgagca
    21421 accagaaggc atcgcgccca cctggaccgt ttcttcgttg cacgaactgg agcagctcct
    21481 gtgtaaacac tgattgcctc ccccccgttg atgggtaaaa tagccgcaat ttttcgtttt
    21541 caacaagcgc ggcgcgatgc cgcttactca agaagaaaga attatgacgt tgtctcctta
    21601 tttgcaagag gtggcgaagc gccgcacttt tgccattatt tctcacccgg atgccggtaa
    21661 gactaccatc accgagaagg tgctgctgtt cggacaggcc attcagaccg ccggtacagt
    21721 aaaaggccgt ggttccaacc agcacgctaa gtcggactgg atggagatgg aaaagcagcg
    21781 tgggatctcc attactacgt ctgtgatgca gtttccgtat cacgattgcc tggttaacct
    21841 gctcgacacc ccggggcacg aagacttctc ggaagatacc tatcgtaccc tgacagcggt
    21901 ggactgctgc ctgatggtta tcgacgctgc aaaaggtgtt gaagatcgta cccgtaagct
    21961 gatggaagtt acccgtctgc gcgacacgcc gatcctcacc tttatgaaca aacttgaccg
    22021 tgatatccgc gacccgatgg aactgctcga tgaagttgag aacgagctga aaatcggctg
    22081 cgcaccgatc acctggccga ttggctgcgg caagctgttt aaaggcgttt accaccttta
    22141 taaagacgaa acctatcttt atcagagcgg taaaggccac acgattcagg aagtccgtat
    22201 tgttaaaggg ctgaataacc cggatctcga cgctgcggtt ggtgaagatc tggcacagca
    22261 gctacgtgac gaactggaac tggtgaaagg cgcgtctaac gagttcgaca aagaactgtt
    22321 ccttgcgggc gaaatcactc cggtattctt cggtactgcg ctgggtaact tcggtgttga
    22381 tcatatgctg gatggcctgg tggagtgggc tcctgcgccg atgccgcgtc agactgatac
    22441 ccgtaccgta gaggcgagcg aagacaaatt taccggcttc gtatttaaaa ttcaggccaa
    22501 catggacccg aaacaccgcg accgcgtggc gtttatgcgc gtagtatccg gtaaatatga
    22561 aaaaggcatg aagctgcgcc aggtgcgtac tgcgaaagat gtggttatct ccgacgcgct
    22621 gacctttatg gcgggcgacc gttcgcacgt tgaagaagcg tatccgggcg atatcctcgg
    22681 cctgcacaac cacggcacta ttcagatcgg cgacaccttt acccagggtg agatgatgaa
    22741 gttcaccggt attccgaact tcgcaccaga actgttccgt cgtatccgcc tgaaagatcc
    22801 gctgaagcaa aaacagctgc tcaaagggct ggtacagctt tccgaagagg gcgcggtgca
    22861 ggtgttccgt ccgatctcca acaacgattt gatcgttggt gctgttggtg tgctgcagtt
    22921 tgatgtggtg gtatcgcgcc tgaagagcga atacaacgtt gaagcagtat atgaatcagt
    22981 caacgttgcc actgcccgct gggtagaatg tgcggacgcg aagaaattcg aagagttcaa
    23041 gcgtaagaac gaaagccaac tggcgcttga tggcggcgat aacctcgctt acatcgctac
    23101 cagcatggtc aacctgcgcc tggcgcagga acgttatccg gacgttcagt tccaccagac
    23161 ccgcgagcat taattcttgc ctccagggcg cggtagccgc tgcgccctgt caatttccct
    23221 tccttattag ccgcttacgg aatgttctta aaacattcac ttttgcttat gttttcgctg
    23281 atatcccgag cggtttcaaa attgtgatct atatttaaca aagtaatgac atttctgacg
    23341 gcgttaaata ccgttcaatg cgtagatatc agtatctaaa gccgtcgatt gtcattctac
    23401 cgatattaat aactgattca gaggctgtaa tggtcgttat tcatcaccca tcgcttttgt
    23461 gatggcgacc attgacttct gtagagggtg aagtctctcc ctattcagca atgcaacctc
    23521 gtgttgccag gctcaaatta cgagcaaaca tacaggaata aatcgatgac tatgacaaga
    23581 ctgaagattt cgaaaactct gctggctgta atgttgacct ctgccgtcgc gaccggctct
    23641 gcctacgcgg aaaacaacgc acagactacc aatgaaagcg cagggcaaaa agtcgatagc
    23701 tctatgaata aagtcggtaa tttcatggat gacagcgcca tcaccgcgaa agtgaaggcg
    23761 gccctggtgg atcatgacaa catcaagagc accgatatct ctgtaaaaac cgatcaaaaa
    23821 gtcgtgaccc tgagcggttt cgttgaaagc caggcccagg ccgaagaggc agtgaaagtg
    23881 gcgaaaggcg ttgaaggggt gacctctgtc agcgacaaac tgcacgttcg cgacgctaaa
    23941 gaaggctctg tgaagggcta cgcgggtgac accgccacca ccagtgaaat caaagccaaa
    24001 ctgctggcgg acgatatcgt cccttcccgt catgtgaaag ttgaaaccac cgacggcgtg
    24061 gttcagctct ccggtaccgt cgattcccag gcacagagcg accgtgctga aagtatcgcc
    24121 aaagcggtag atggtgtgaa aagcgttaaa aatgatctga aaactaagta attcgtcgta
    24181 attcgtcctc ccgaactttg tcgggaggcg cgatgtgcac cacactaaaa atatcgcatt
    24241 gagtgatttt cacgctcata tcaagcggtg acattaacta tggtaaagga gacgcttatg
    24301 tttcgttggg gcatcatatt tctggttatc gcgttaatcg ccgccgcact tgggtttggt
    24361 ggtctggccg gtaccgctgc aggcgcagct aaaattgtct ttgtcgtcgg gattattctg
    24421 ttcctggtga gtttgttcat gggccgaaaa cgaccctaga tttcagacca ttaagaaaat
    24481 tttatccaaa gccagtccag cggactggct tttgcggttt tagcgaataa taaattgcgt
    24541 tactttgtca ttctttcaca acaaaaacag gaaggcagag gtggggcagc gaatacctgt
    24601 aacgcttggt aatattgcgc cgttgtcgct aaggccgttc cagcctggac gaatagctct
    24661 ggtgtgcgaa ggcggcggac agcgtggaat tttcacggct ggcgtgctgg atgagtttat
    24721 gcgcgcgcag tttaatcctt tcgatcttta tctcggcaca tctgccgggg cgcagaacct
    24781 ctcggcgttt atctgcaatc agcccggtta cgcgcgcaaa gtcatcatgc gctataccac
    24841 aaaacgcgaa tttttcgatc cattgcgctt tgtccgtgga ggaaatctta tcgatctcga
    24901 ctggctggtg gaggccaccg caagccagat gccgttgcaa atggataccg ccgcgcggtt
    24961 gtttgacagc ggcaaatcgt tttatatgtg cgcctgtcgt caggatgact acgcgcctaa
    25021 ttacttttta ccaaccaaac aaaactggct ggatgtgatt cgcgcctcca gtgcgatacc
    25081 tggcttttat cgtagcggag tgtcgctgga aggcattaac tacttggatg gcgggatcag
    25141 tgatgcgatt ccggttaaag aggcggcaag gcagggcgct aaaacgttgg tcgtcatccg
    25201 cactgtgccg tcacaaatgt actacacgcc gcagtggttc aaacgcatgg aacgctggct
    25261 gggtgacagt agcctgcagc cgctggtcaa tctggtgcag catcatgaaa ccagctatcg
    25321 tgacattcag caatttattg agaaaccacc gggcaagctg cggatattcg aaatttatcc
    25381 accgaaacca ttacatagta tcgcgcttgg cagtcggatt ccggcgctgc gtgaagacta
    25441 taaacttggg cgtttatgcg gtcgttattt cctcgccacg gttggcaagc tattaactga
    25501 aaaagcgccg cttacccgcc atctggtgcc agtggtgacg ccggaatcga tcgttattcc
    25561 gcctgcgcca gtcgccaacg atacgctggt tgccgaagtg agcgatgctc cgcaggcgaa
    25621 cgacccgaca tttaacaatg aggatctggc ttgatttgcc gttttatcga tacccactgc
    25681 cattttgatt tcccgccgtt tagtggcgat gaagaggcca gcctgcaacg cgcggcacaa
    25741 gcgggcgtag gcaagatcat tgttccggcg acagaggcgg aaaattttgc ccgcgtgcag
    25801 gcgttagcgg aaaaatatca accgctgtat accgcattgg ggttgcatcc ggggatgttg
    25861 gaaaaacata gcgatgtgtc tcttgatcag ctacagcagg cgctggaaag gcgtccggcg
    25921 aaggtggtgg cggtggggga gatcggtctg gatctcttcg gcgacgatcc gcaatttgag
    25981 aggcagcagt ggttactcga cgaacagctg aaactggcga aacgctacga tctgccggtg
    26041 atccttcatt cccggcgtac acacgacaaa ctggcgatgc atcttaaacg tcacgattta
    26101 ccgtgcactg gcgtggttca cggtttttcc ggcagcctgc aacaggccga gcggtttgta
    26161 cagctgggct acaaaatcgg cgtaggcggt actatcacct atccacgcgc cagtaaaacc
    26221 cgcgatgtca tcgcaaaatt accgctggca tcgttgttgc tggaaaccga cgcgccggat
    26281 atgccgctca acggctttca ggggcagcct aaccgaccgg aacaggctgt gcgggtgttt
    26341 gacgtgcttt gcgagttgcg cccggaacca gaagatgaga ttgccgaagt gttgcttaat
    26401 aatacgtatg cgttgtttag cgttagtggg tagtttaccc ttcgtaggca gcatccggca
    26461 gtcggcgcat aatgcctgat gcgacgctta acgcgtctta tcatgcctac aggtttgtgc
    26521 cgaaccgtag gccggataag gcgttcacgc cgcatccggc agttggcgca caatgcctga
    26581 tgcgacgctt gacgcgtctt atcatgccta caggtttgtg ccgaaccgta ggccggataa
    26641 ggcgttcacg ccgcatccgg cggtcggtgc acaatgccag atgcgacgct ggacgcgtct
    26701 tatcatgcct acaagtctgt gccgaaccgt aggccggata aggcgttcac gccgcatccg
    26761 gcagtcggcg cataatgcct gatgcgacgc ttgtcgcgtc ttatcatgcc tacaagtctg
    26821 tgccgaaccg taggccgtat ccggcatgtc acaaatagag cgccggaaat atcaaccggc
    26881 tcaccccgcg cacctttaac gcatcagcca acggctcaac gtcttccggc gtggcgctcg
    26941 cccagctttg cgcctcgcca tacacgccgt gggcatgaaa cgcattcagg cgaaccggaa
    27001 catcgccaag tctcttgata aacgtcgcca gctcatcgat gtgatgcaaa taatccacct
    27061 ggtctggaat taccagcaaa cgcagttccg ccagcttgcc gcgctctgca agcaaacaga
    27121 tgctgcgctt aatctgctga ttatcgcgtc cggttaagtg ttgatgacat tcgctccccc
    27181 acgctttgag atcgagcatt gcgccgtcac acaccgggag taatttttcc cagccggttt
    27241 cgctcaacat gccgttactg tccaccaggc aggtgaggtg gcgcagttgc ggatcgtttt
    27301 tgatagcagt aaacagcgcc actacaaacg gcagttgggt cgtggcttcg ccgccgctta
    27361 ccgttatccc ttcaataaac agcaccgctt tgcggacatg gctaagcact tcgtccacgc
    27421 tcatggattg cgccattggc gtggcatgtt gcggacacat cttcaggcag gtatcacact
    27481 gctcgcagac cacagcgttc cacagcactt tgccgtcaac aatctgcaac gcctgatgcg
    27541 gacactgtgg cacgcactct ccgcaatcat tgcagcgtcc catcgtccac ggattgtgac
    27601 agtttttgca gcgcagattg cagccctgca aaaacagagc cagacgactg cctggcccgt
    27661 caacgcagga gaaggggata atcttactga ctaaagcgca tctgctgttc atggcttatc
    27721 acgcgcggct ggcgttccag aatacgagtg ttgcgtgcgg cttcttcgcc cagccaggtg
    27781 gtgttggtgc gtgaaccttc ggcgcgatat ttttctaagt ccgacaaacg taccatataa
    27841 ccggtaacgc gaaccagatc gttaccgctg acattggcgg taaattcacg cattccggct
    27901 ttaaaggcac cgaggcaaag ctgtaccagt gcctgcgggt tacgtttgat ggtttcgtcg
    27961 agcgtcagaa tgtcgctgat gccggaataa taataagcat gatgcggggc gacagtttgc
    28021 agatgggtga tcggatctgg ctcatcgcca tacggcaaac gcgcgcccgg cgtggtgcca
    28081 atatcggaac tgatccccga ctgtgcgtgt aacatggcgc gtttttgcca tccatatttc
    28141 acgggggtat tggcgacaaa ctccgccagt tgcgcgctga tgcgataacc cactttattt
    28201 gcggcggctt ctttaccgta acgtgcggca atcccttctt tctcacacag caagttaacc
    28261 gcttccgcca gcccatacat gccaaacatt ggcacaaaac gttcagggtt aatcagccct
    28321 tctttcacca ggaagctatt ctcaaagaag tgtgattgtt gatagaggaa ttcacaccgc
    28381 gcatcgatga tggcgatctg ctgctggcag tagtgcggta gagtgcgcgt aaagaagtca
    28441 tccagcgatt cgctgcgctc ggcaatggct ttcaggttaa ggcgtaccag cgtgctgcca
    28501 ccacccgcca gcggcagtga gttgtaacag ctcacaatcc cgtagccccc ttttgtgaaa
    28561 attttatcat gcaccggacc gttggcgatg tgcggtttgc tacattcaca gatgttcttc
    28621 gccacttcca gcagcaggtc atcaggggtg atttcaggat cgtagataaa ggtcaggttc
    28681 ggtgaaacct gcttcaactc tgcgtctgca cgtaagattg cacgggtaat gggcgaatca
    28741 gacgggccga tattggcgtg cataaaggcg tctggcaggg ttctgtcgag gtaacgccag
    28801 aaacgtttta ttcgaacatc gatctcgtct tgtgttagaa ttctaacata cggttgcaac
    28861 aacgcatcca gttgccccag gtagaccggc atcgatgtga ccgacggtac gtggtggtaa
    28921 agaatggtca gcagagagag tgcgtcatca agatctttcg cgccttccag ctccagccat
    28981 tcggaaccgt tcgccagaaa acgggcgtaa tcgggtaaga catagcgcgg tttgtacggc
    29041 gcatgacctt caaacatatc gcagattaca ccttcatcca gcgcgcggcg ggcttcggca
    29101 ggcagctgtg ggtaaggcag attgttttct gcttccagtg ccagaaaatg gcgcttctgc
    29161 tccgggctaa gcactgggct ggtgacaatt tgctggcaac gttgttgcag tgcattttca
    29221 tgagaagtgg gcatcttctt ttccttttat gccgaaggtg atgcgccatt gtaagaagtt
    29281 tcgtgatgtt cactttgatc ctgatgcgtt tgccaccact gacgcattca tttgaaagtg
    29341 aattatttga accagatcgc attacagtga tgcaaacttg taagtagatt tccttaattg
    29401 tgatgtatat cgaagtgtgt tgcggagtag atgttagaat actaacaaac tcgcaaggtg
    29461 aattttattg gcgacaagcc aggagaatga aatgactgat ctgaaagcaa gcagcctgcg
    29521 tgcactgaaa ttgatggacc tgaccaccct gaatgacgac gacaccgacg agaaagtaat
    29581 tgctctgtgt catcaggcca aaaccccggt cggcaatacc gccgctatct gtatctatcc
    29641 tcgctttatc ccgattgctc gcaaaacact gaaagagcag ggcaccccgg aaatccgtat
    29701 tgctacggta accaacttcc cacacggtaa cgatgacatc gaaatcgcgc tggcagaaac
    29761 ccgtgcggca atcgcctacg gtgccgatga agttgacgtg gtgttcccgt accgcgcgct
    29821 gatggcgggt aacgagcagg ttggttttga cctggtgaaa gcctgtaaag aggcttgcgc
    29881 ggcagcgaat gtactgctga aagtgatcat cgaaaccggc gaactgaaag acgaagcgct
    29941 gatccgtaaa gcgtctgaaa tctccatcaa agcgggtgcg gacttcatca aaacctctac
    30001 cggtaaagtg gctgtgaacg cgacgccgga aagcgcgcgc atcatgatgg aagtgatccg
    30061 tgatatgggc gtagaaaaaa ccgttggttt caaaccagcg ggcggcgtgc gtactgcgga
    30121 agatgcgcag aaatatctcg ccatcgcaga tgaactgttc ggtgctgact gggcagatgc
    30181 gcgtcactac cgctttggtg cttccagcct gctggcaagc ctgttgaaag cgctgggcca
    30241 cggtgacggt aagagcgcca gcagctacta agtaagatgc tttacgcctg atgcgctgcg
    30301 cttatcaggc ctacgagacg tatctacccg taggccggat aaggcgtaga cgcatccggc
    30361 aaaagccgcc tcatactctt ttcctcggga ggttaccttg tttctcgcac aagaaattat
    30421 tcgtaaaaaa cgtgatggtc atgcactgag cgatgaagaa attcgtttct ttatcaacgg
    30481 cattcgcgac aacactatct ccgaagggca gattgccgcc ctcgcgatga ccattttctt
    30541 ccacgatatg acaatgcctg agcgtgtctc gctgaccatg gcgatgcgag attcaggaac
    30601 cgttctcgac tggaaaagcc tgcatctgaa tggcccaatt gttgataaac actccaccgg
    30661 tggcgtcggc gatgtgactt cgctgatgtt ggggccgatg gtcgcagcct gcggcggcta
    30721 tattccgatg atctccggtc gcggcctcgg tcatactggc ggtacgctcg acaaactgga
    30781 atccattcct ggcttcgaca ttttcccgga tgacaaccgt ttccgcgaaa ttattaaaga
    30841 cgtcggcgtg gcgattatcg gtcagaccag ctcactggct ccggcggata aacgtttcta
    30901 cgcgacccgt gatattaccg caaccgtgga ctccatcccg ctgatcactg cctcgattct
    30961 ggcgaagaaa ctggcggaag gtctggacgc gctggtgatg gacgtgaaag tgggtagcgg
    31021 cgcgtttatg ccgacctacg aactctctga agcccttgcc gaagcgattg ttggcgtggc
    31081 taacggcgct ggcgtgcgca ccaccgcgct gctcaccgac atgaatcagg tactggcctc
    31141 cagtgcaggt aacgcggttg aggttcgtga agcagtgcag ttcctgacgg gagaatatcg
    31201 taacccgcgt ctgtttgatg tcacgatggc gctgtgcgtg gagatgctga tctccggcaa
    31261 actggcgaaa gatgacgccg aagcacgcgc gaaattgcag gcggtgctgg acaacggtaa
    31321 agcggcagaa gtctttggtc gtatggtagc ggcacaaaaa ggcccaaccg acttcgttga
    31381 gaactacgcg aagtatctgc cgacagcgat gctgacgaaa gcagtctatg ctgataccga
    31441 aggttttgtc agtgaaatgg atacccgcgc gctggggatg gcagtggttg caatgggcgg
    31501 cggacgccgt caggcatctg acaccatcga ttacagcgtc ggctttactg atatggcgcg
    31561 tctgggcgac caggtagacg gtcagcgtcc gctggcggtt atccacgcga aagacgaaaa
    31621 cagctggcag gaagcggcga aagcggtgaa agcggcaatt aaacttgccg ataaagcacc
    31681 ggaaagcaca ccaactgtct atcgccgtat cagcgaataa cggtatactg atctgatcat
    31741 ttaaatttga agcactgagt acggagaaca tatgaaacgt gcatttatta tggtgctgga
    31801 ctcattcggc atcggcgcta cagaagatgc agaacgcttt ggtgacgtcg gggctgacac
    31861 cctgggtcat atcgcagaag cttgtgccaa aggcgaagct gataacggtc gtaaaggccc
    31921 gctcaatctg ccaaatctga cccgtctggg gctggcgaaa gcacacgaag gttctaccgg
    31981 tttcattccg gcgggaatgg acggcaacgc tgaagttatc ggcgcgtacg catgggcgca
    32041 cgaaatgtca tccggtaaag ataccccgtc tggtcactgg gaaatcgccg gtgtcccggt
    32101 tctgtttgag tggggatact tctccgatca cgaaaacagc ttcccgcaag agctgctgga
    32161 taaactggtc gaacgcgcta atctgccggg ttacctcggt aactgccact cttccggtac
    32221 ggtcattctg gatcagctgg gcgaagagca catgaaaacc ggcaagccga ttttctatac
    32281 ctccgctgac tccgtgttcc agattgcctg ccatgaagaa actttcggtc tggataaact
    32341 ctacgaactg tgcgaaatcg cccgtgaaga gctgaccaac ggcggctaca acatcggtcg
    32401 tgttatcgct cgtccgttta tcggcgacaa agccggtaac ttccagcgta ccggtaaccg
    32461 tcacgacctg gccgttgaac cgccagcacc gaccgtgctg cagaaactgg ttgatgaaaa
    32521 acacggccag gtggtttctg tcggtaaaat tgcagacatc tacgccaact gcggtatcac
    32581 caaaaaagtg aaagcgactg gcctggacgc gctgtttgac gccaccatca aagagatgaa
    32641 agaagcgggt gataacacca tcgtcttcac caacttcgtt gacttcgact cttcctgggg
    32701 ccaccgtcgc gacgtcgccg gttatgctgc gggtctggaa ctgttcgacc gccgtctgcc
    32761 ggagctgatg tctctgctgc gcgatgacga catcctgatc ctcaccgctg accacggctg
    32821 tgatccgacc tggaccggta ctgaccacac gcgtgaacac attccggtac tggtttacgg
    32881 cccgaaagta aaaccgggct cactgggtca ccgtgaaacc ttcgcggata tcggccagac
    32941 tctggcaaaa tattttggta cttctgatat ggaatatggc aaagccatgt tctgatggat
    33001 ttgggcggag cgttgactcc gcctttgtta tgtcacaaaa aggataaaac aatggctacc
    33061 ccacacatta atgcagaaat gggcgatttc gctgacgtag ttttgatgcc aggcgacccg
    33121 ctgcgtgcga agtatattgc tgaaactttc cttgaagatg cccgtgaagt gaacaacgtt
    33181 cgcggtatgc tgggcttcac cggtacttac aaaggccgca aaatttccgt aatgggtcac
    33241 ggtatgggta tcccgtcctg ctccatctac accaaagaac tgatcaccga tttcggcgtg
    33301 aagaaaatta tccgcgtagg ctcctgtggc gctgttctgc cacacgtaaa actgcgcgac
    33361 gtcgttatcg gtatgggtgc ctgcaccgat tccaaagtta accgcatccg ttttaaagac
    33421 catgactttg ccgctatcgc tgactttgac atggtgcgta acgcagtaga tgcagctaaa
    33481 gcactgggca ttgatgctcg cgttggtaac ctgttctccg ctgacctgtt ctactctccg
    33541 gacggcgaaa tgttcgacgt gatggaaaaa tacggcatcc tcggcgtgga aatggaagcg
    33601 gctggtatct acggcgtcgc tgcagaattt ggcgcgaaag ccctgaccat ctgcaccgtg
    33661 tctgaccaca tccgtactca cgagcagacc actgccgctg agcgtcagac caccttcaac
    33721 gacatgatca aaatcgcact ggaatccgtt ctgctgggcg ataaagagta attgtgtttc
    33781 gctgaaaggc gattgccttg tgaagccgga acgggagact gctccggctt tttagtatct
    33841 attcatcaat agcgactgat ttatagattt actattattt ttcatatgga tagaaaataa
    33901 aatccattca tgaatctatt catttatgtt cagccgtcat ccagtaaggg tatatcagca
    33961 actctggtgg acaaccataa attcctgcca ttttcttttg atgtttgagc taagccgttc
    34021 cgtcttttcg atccgtttta gagaattaat cgagatacct aacattttac ttacttcagc
    34081 aagcgataat ttatgcctga tacgcacggc ggctagccag gatataccct ggctgaacat
    34141 taaattgata acctcccgtt cctggttttc cagatcgatc atatttcctt ccctggatat
    34201 tttaatgagc gagttaccgt accgcccccg ctatccatgc cgataactcc cgtagctctt
    34261 tttcctgttc cgggaagtca accaacagcg cttcacactc ctgttgcagc atgtcagcgc
    34321 ggtacaggca gccttgcagc cgtcctgcga gggcttccag tggtgctggg ttaaggctgt
    34381 cggtaaatac ctgggcgcgg gtgatatggc ctttttcaac gtcgaaatgc agttccacgc
    34441 cgccccaggt aaagcgttca tccagcagat gcgagaatgc cggagcctga ccgaagttcc
    34501 actcccagct actctgacgg gcaaaggttt cggcgaagtt cggcaagtct ggcgttttgt
    34561 ccggggagat gatttccgct tccacgtgct cgccataatg ggcgaaaaag gcctcggtta
    34621 tggcctcgca aacctgctca tgggtgatcc ccggcagcaa ttcggtcaga ttggtcacgc
    34681 gggaacgtac cgacgtaatg cctttcgccg ccagtttctt tttatccgga ttgagatagt
    34741 ttgccaggcg gctaaggtcg gcattgagca gcaaggtgcc gtggtggaag ccgcgatctt
    34801 tggtttcgcg ataggccgag cctgagactt tgcggtcgcc ttcggcggtt ttcaccacca
    34861 gatcgttacg tccggacgct tcggcgctga cgccgagcgc gttcagcgca ttgagcacaa
    34921 tcgacgtgga gatggtttta tcgtactccg gcttgccagc cataaaggta aagcaggtat
    34981 tgccgagatc gtggaacact gcgccgccac cgctgctgcg ccgcgccagg cggacgttat
    35041 cttcttccat ccgccgggta ttacactctt tccacgggtt ctgtgcgcga ccaatcacta
    35101 ccgtgtcggc attgcgccag agaaacagaa cgcgctgcgt ggcgggcatc tggcgaaaaa
    35161 tacactcttc caccgccagg ttaaaccacg ggtcgtaaga gtcagagatg agcaggcgta
    35221 atgtggacat aacgatttcc tttcttgtag taatgggtca ctcttttttc tcgctttctt
    35281 cctcttccgg caccggtttg ctggcggtta acaggaaggg cgattgctgc cagcgggtgc
    35341 gtttaccctg tagcagcgtg cgggtcagca ctacgccgat tgccagcgag agcagcaaca
    35401 tcaggcgtaa aatgttagtg gtgttatcca cctgttgggc ttcggtggcg agcgtatggg
    35461 tgtcgagcgt caggcgcaga tagccgagcg gtccgttttt acccgcaatg ggttcgacaa
    35521 tctgctggtt aaaatagccg cccgcttttt tgccgtcgag cgccagccgg tcgcgcacgt
    35581 cgacgctttc accagaacgg gcgataagat cgccttgttc atcatatacg cctgcgtcga
    35641 gaatgcggct ttcatccgtt aactgatcga ggatcgcctg aatacgtttt tcatccggtg
    35701 agtcggtacg catcagcggt gcaacgttca gcgttacctg acgcgccagg gtgcgggcta
    35761 gttcttccag ctgtggatta cgctgtcgct ggtggttttg actaaaccat gacgccccct
    35821 gcatcagcgc cactaacaag gcaagacaga acaggacaat cactgcccga tgcagccgga
    35881 atttcagttt tgtgcgagcc atcttccacc ctttgaaaat ttgagactta atgttgccag
    35941 aagcaatgga tacaaggtag cctcatgcgt tattttccct gcttcgaacg attttacagg
    36001 agccttaatg cctaacatta cctggtgcga cctgcctgaa gatgtctctt tatggccggg
    36061 tctgcctctt tcattaagtg gtgatgaagt gatgccactg gattaccacg caggtcgtag
    36121 cggctggctg ctgtatggtc gtgggctgga taaacaacgt ctgacccaat accagagcaa
    36181 actgggcgcg gcgatggtga ttgttgccgc ctggtgcgtg gaagattatc aggtgattcg
    36241 tctggcaggt tcactcaccg cgcgggctac gcgcctggcc catgaagcgc aactggatgt
    36301 cgcgccgctg gggaaaatcc cacacctgcg cacgccgggt ttgctggtga tggacatgga
    36361 ttccaccgcc atccagattg aatgtattga tgaaattgcc aaactggccg gaacgggcga
    36421 gatggtggcg gaggtaaccg aacgggcgat gcgcggcgaa ctcgatttta ccgccagcct
    36481 gcgcagccgc gtggcgacgc tgaaaggcgc tgacgccaat attctgcaac aggtgcgtga
    36541 aaatctgccg ctgatgccag gcttaacgca gctggtactc aagctggaaa cgctgggctg
    36601 gaaagtggcg attgcctccg gtggctttac tttctttgct gaatacctgc gcgacaagct
    36661 gcgcctgaca gccgtggtag ccaatgaact ggagatcatg gacggtaaat ttaccggcaa
    36721 tgtgatcggc gacatcgttg acgcgcaata taaagcgaaa accctgaccc gcctcgcgca
    36781 ggagtatgaa atcccgctgg cgcagaccgt ggcgattggc gatggagcca atgacctgcc
    36841 gatgatcaaa gcggcagggc tggggattgc ctaccatgcc aagccaaaag tgaatgaaaa
    36901 gacggaagtc accatccgtc acgctgacct gatgggggta ttctgcatcc tctccggcag
    36961 cctgaatcag aagtaattgc ccgcccgtca tcctgcgggc ggcacagcat taacgaggta
    37021 catcgtggca aaagctccaa aacgcgcctt tgtttgtaat gaatgcgggg ccgattatcc
    37081 gcgctggcag gggcagtgca gtgcctgtca tgcctggaac accatcaccg aggtgcgtct
    37141 tgcagcgtcg ccaacggtgg cgcgtaacga gcgtctcagc ggctatgccg gtagcgccgg
    37201 ggtggcaaaa gtccagaaac tttccgacat cagccttgaa gagctgccgc gtttttccac
    37261 cggatttaaa gagttcgacc gcgtattagg cggcggcgtg gtgccgggaa gtgccattct
    37321 gattggcggt aaccctggtg cggggaaatc cacgttgcta ctgcaaacgc tgtgcaaact
    37381 ggcgcagcag atgaaaacgc tgtatgtcac cggcgaagag tcgctgcaac aggtggcaat
    37441 gcgcgctcat cgccttggcc tgccgactga caatcttaat atgctgtcgg aaaccagcat
    37501 cgagcagatc tgcctgattg ccgaagaaga gcaaccgaag ctgatggtaa ttgactcgat
    37561 ccaggtgatg catatggcgg atgtacaatc atcgcctggt agcgtggcgc aggtgcgtga
    37621 aacggcggct tatctgacgc gcttcgccaa aacgcgcggc gtggcgattg tcatggtcgg
    37681 gcacgtaacc aaagatggct cgctggctgg ccctaaagtg ctggaacact gtatcgactg
    37741 ttcggtgctt ttggatggcg atgccgactc ccgttttcgc accttgcgca gccataaaaa
    37801 ccgcttcggc gcggtgaatg agctgggcgt cttcgcgatg accgaacagg ggctgcgtga
    37861 agttagcaac ccttccgcca tctttttaag ccgcggtgat gaagtgacct ccggtagctc
    37921 agtgatggtg gtatgggaag gaacgcgtcc actgctggtg gagatccagg cgctggtcga
    37981 tcactcgatg atggcgaatc cacgccgcgt cgcagtaggg ctggagcaaa accgtctggc
    38041 aatcctgctg gctgtcttgc accgtcacgg tggtctgcaa atggccgatc aggatgtgtt
    38101 tgtgaacgtg gtcggcggcg tgaaggtaac cgaaaccagc gccgacttag cgttactgct
    38161 ggcgatggtt tccagcctgc gtgacagacc gctgccacag gatctggtgg tgtttggtga
    38221 agtcgggctg gcaggggaga tccgcccggt gcccagcggc caggaacgaa tctctgaagc
    38281 ggcgaaacac ggttttcggc gggcgattgt tccggcggct aatgtgccga aaaaagcgcc
    38341 ggaagggatg cagatttttg gcgttaaaaa actctccgac gcgcttagcg tgttcgacga
    38401 cttataatga gagatacgga gggagatatg tcgtcatttg attacctgaa aactgccatc
    38461 aagcaacagg gctgcacgct acagcaggta gctgatgcca gcggtatgac caaagggtat
    38521 ttaagccagt tactgaatgc caaaattaaa agccccagcg cgcaaaaact ggaggcattg
    38581 caccgttttt tggggcttga gtttccccgc caaaagaaaa ccattggcgt ggtattcggt
    38641 aagttctacc cgttgcatac cggacatatc taccttatcc agcgcgcctg tagccaggtt
    38701 gacgagttgc atatcattat gggctttgac gatacccgtg accgtgcgtt gtttgaagac
    38761 agcgccatgt cgcagcagcc cacggtgccg gatcgtctgc gctggttatt gcaaaccttt
    38821 aaatatcaga aaaatatccg tattcatgct ttcaatgaag agggcatgga gccatatccg
    38881 cacggctggg atgtatggag caacggcatc aaaaagttta tggctgaaaa ggggatccag
    38941 ccggacctga tctacacctc ggaagaagcc gatgcgccac agtatatgga acatctgggg
    39001 atcgaaacgg tgctggtcga tccgaaacgc acctttatga gtatcagcgg tgcgcagatc
    39061 cgcgaaaacc cgttccgcta ctgggaatat attcctacgg aagtgaagcc gttctttgta
    39121 cgtaccgtgg cgatccttgg tggcgagtcg agcggtaaat ccaccctggt aaacaaactt
    39181 gccaatatct tcaataccac cagtgcgtgg gaatatggtc gcgattatgt cttttcacac
    39241 cttggcggtg atgagatcgc attgcagtat tccgactacg ataaaatcgc gctaggacac
    39301 gcgcaatata ttgattttgc agtgaaatac gccaataaag tggcgtttat cgacactgat
    39361 tttgtcacta cccaggcgtt ctgcaaaaag tacgaagggc gtgagcatcc gttcgtgcag
    39421 gcgctgattg atgaataccg ttttgatctg gttatcttgc tggagaacaa cacgccgtgg
    39481 gtggcggatg gtttacgcag cctcggtagt tcggtggatc gcaaagagtt ccagaacttg
    39541 ctggtggaga tgctggaaga gaacaatatc gaatttgtgc gtgttgaaga ggacgattac
    39601 gacagccgtt tcctgcgctg tgtggagctg gtgcgggaga tgatgggcga gcagagataa
    39661 ccgtgacgaa acggctcaaa ggcgaggtat aaattgagtt tttttgatga gttgaaaacc
    39721 tctctggaag aggctgtcga gattaaacaa ggtttaaaag aatcagcacg ggtgacccgc
    39781 tacgaatttg cggatactaa ggctgttgta ggccggaaaa ggcgttcacg ccgtatccgg
    39841 cattcggtgc tcaatgcctg atgcgacgct taccgcgtct tatcaggcct ggagattcac
    39901 aacgtattga aatttcttgg tttgtaggcc ggataaggcg ttcacgccgc atccggcatt
    39961 ttacgcatta cttcgcaata cgcttgtact tgatacgctt cggctccagt gcgtctgcgc
    40021 ccagcgtgcg tttcttgtac tcttcgtact cagtaaagtt accttcgaag aactcaactt
    40081 taccttcatc ctggtagtcc aggatgtgcg tggcgatacg gtcgaggaac caacggtcgt
    40141 gcgagataac catcgcacag cccgggaact ccagcagggc gttttccagc gcgcgcaggg
    40201 tttcgatatc caggtcgttg gttggttcgt cgagcagcag catgttgccg ccaacctgca
    40261 gcagcttcgc cagatgcaga cgaccgcgct caccaccgga aagttcacca acgcgtttac
    40321 cctgatcaac ccctttaaag ttaaagcggc caacgtaggc gcggcttggc atctcggtgt
    40381 tgccgatctt cataatatcc agcccgccgg aaacttcttc ccaaacggtt ttgctgttat
    40441 ccattgagtc acggaactga tcaaccgatg ccagtttcac cgtttcacct aaagtgatgg
    40501 tgccgctgtc cggctgttcc tgaccagaga tcatacggaa cagggtcgat ttacccgcgc
    40561 cgttcggacc gatgatcccg acgattgccc ctttcgggat cgagaagctc aggtcatcaa
    40621 tcagcaggcg atcaccgtag gatttacgca ggttgctgac ttccagcact ttatcgccca
    40681 gacgcggtcc aggtggaata aacagttcgt tggtttcgtt acgtttctga tattcggtgc
    40741 tgttcagctc ttcaaagcgt gccagacgtg ctttaccttt cgactggcgg cctttagttc
    40801 cctgacgtac ccactccagc tctttctcga tcgacttacg acgcgccgct tcttgtgaag
    40861 cttcctgcgc caggcgctga tctttctgct ccagccagga ggagtagttg ccttcccacg
    40921 gaataccttc accgcggtca agttcgagga tccagcctgc aacgttatcg aggaagtaac
    40981 ggtcgtgagt aatcgccaca acggtgcctt cgaagtcgtg caggaagcgt tccagccagg
    41041 ccacggattc ggcatccagg tggttggtcg gttcgtcgag cagcagcatg tctggttttt
    41101 ccagaagcag gcggcacaac gccacgcgac gacgctcacc accggagagg ttagcgattt
    41161 tcgcgtccca atctggcaga cgcagcgcat ccgccgcacg ctccagctgt acgttcaggt
    41221 tatgaccgtc gtgagcctga atgatctctt ccagacggcc ttgttcagcg gccagcttgt
    41281 caaaatcggc atccggatcg gcgtacagcg catacacttc atccaggcgt ttcagggcgt
    41341 taaccacttc agaaaccgct tcttcaatgg actcacgcac ggtgtgttcc gggttcagct
    41401 gcggttcctg cggcaggtaa ccaatcttga tgtctggctg cggacgcgct tcaccttcga
    41461 tgtctttatc aatgcccgcc ataatgcgca gcagggtaga cttacccgcg ccgttcagac
    41521 ccaggacacc aatttttgcc ccagggaaga aactcagaga gatgtttttc aaaatatgac
    41581 gtttcggcgg aacaactttg ccgacacgat gcatggtata aacgaattga gccacgttgg
    41641 acttcgcctc tatgtttatc gtgatgatga gttttcaaag gcgaagtgta gcctttttcc
    41701 ccgcctaatc ccagttggtc gatcaccttc gcagtaaaag taaaaaagtg tccgtaacgt
    41761 ggcgtaaacg gcaatgactg gttagcataa atctattacg cggcatgacg ctgcattgat
    41821 gtatttacac ttagaggatg cgcttgtgga aaaagccaaa caagttacct ggcggctgtt
    41881 ggctgccggt gtctgtctgc tgacggtcag cagcgtggcg cgagccgact cactggatga
    41941 gcagcgtagc cgttacgcgc aaatcaagca ggcctgggat aatcgacaaa tggatgtggt
    42001 cgaacaaatg atgcctggac tgaaggatta tccgctttat ccctacctgg aataccgcca
    42061 gatcaccgat gatctgatga atcaaccggc ggtgacggtc actaactttg ttcgcgctaa
    42121 ccccacgctt cctcccgctc gcacgctgca atctcgtttc gtcaatgaac tggcgcggcg
    42181 tgaagactgg cgtggcttgt tagcctttag cccggaaaag cccggaacta ccgaagcgca
    42241 atgtaattac tactatgcga aatggaacac cgggcagagt gaagaagcct ggcaaggggc
    42301 gaaagagctg tggctaaccg gcaagagcca gcctaacgcc tgtgacaagt tatttagcgt
    42361 ctggcgtgcg tcaggtaaac aagatccgct ggcgtattta gagcgtatcc gtctggcgat
    42421 gaaagcgggt aacactggcc tggtaacagt gctggcaggg cagatgcctg ccgattacca
    42481 gactatcgcc tcggcaatca tttcactggc gaacaaccct aatacggtac tgaccttcgc
    42541 gcgtacaacc ggagcgaccg attttacccg tcaaatggca gcggtggcgt ttgccagtgt
    42601 ggcgcggcag gatgctgaaa atgcacggct gatgatcccg tcgcttgccc aggcgcagca
    42661 gcttaatgaa gatcagattc aggagctgcg cgatatcgtc gcctggcgtt tgatgggcaa
    42721 cgatgtcacc gacgagcagg cgaaatggcg cgatgacgcc attatgcgct cgcaatccac
    42781 gtcgttgatt gaacgccgtg tgcgaatggc acttggcacc ggcgatcgtc gcggcctgaa
    42841 tacctggctg gcgcgtctgc cgatggaagc aaaagagaaa gatgaatggc gttactggca
    42901 ggcggattta ttgctggaac gcggacgtga agctgaagcg aaagagattt tgcatcaact
    42961 catgcaacag cgtggtttct acccgatggt tgcagcacaa cgcatcggag aagagtatga
    43021 gctgaagatt gataaagcgc cgcagaatgt tgacagcgct ctgactcagg ggccggagat
    43081 ggcgcgcgtg cgcgagttga tgtactggaa tctcgataac accgcgcgta gcgagtgggc
    43141 caatctggtg aagagcaagt caaaaacaga gcaggctcaa ctggctcggt atgctttcaa
    43201 taaccaatgg tgggatctta gcgttcaggc aacgatcgcc gggaagcttt gggatcatct
    43261 ggaagagcga ttcccgctgg cttacaacga tcttttcaaa cgttacacca gtgggaaaga
    43321 gatcccgcaa agctatgcga tggcgattgc ccgtcaggag agcgcctgga atccgaaagt
    43381 gaaatcaccg gtaggggcca gcggcctgat gcagattatg cctggtacag cgacccatac
    43441 ggtgaagatg ttctctattc ctggttatag cagccctggg caattgctgg atccggaaac
    43501 gaatatcaac attggcacca gttacctgca atatgtttat cagcagtttg gcaataaccg
    43561 tattttctcc tcagcagctt ataacgccgg acctggacgg gtgcgaacct ggcttggcaa
    43621 cagcgccggg cgtatcgacg ctgtggcatt tgttgagagt attccgttct ccgagacgcg
    43681 cggttatgtg aagaacgtgc tggcttatga cgcttactac cgctatttca tgggggataa
    43741 accgacgttg atgagcgcca cggaatgggg acgtcgttac tgatccgcac gtttatgata
    43801 tgctatcgta ctctttagcg agtacaaccg ggggaggcat tttgcttccc ccgctaacaa
    43861 tggcgacata ttatggccca acaatcaccc tattcagcag cgatggcaga acagcgtcac
    43921 caggagtggt tacgttttgt cgacctgctt aagaatgcct accaaaacga tctccattta
    43981 ccgttgttaa acctgatgct gacgccagat gagcgcgaag cgttggggac tcgcgtgcgt
    44041 attgtcgaag agctgttgcg cggcgaaatg agccagcgtg agttaaaaaa tgaactcggc
    44101 gcgggcatcg cgacgattac gcgtggatct aacagcctga aagccgcgcc cgttgagctg
    44161 cgccagtggc tggaagaggt gttgctgaaa agcgattgat tttgtaggcc tgataagacg
    44221 cggcagcgtc gcatcaggcg cttaatacac ggcattatga aacggactca gcgccaggat
    44281 caccgcctgg tgatagacgc tggcgcgggt aagtttcccg gcggtaaaca cgccgatcgc
    44341 cccttcctta cgaccaatct catcaatacc ggtataacgc gacatcacgg gaccgagcgc
    44401 ctcgccttca cgcactttct gcaaaatcac cgcaggtaac ggcaaagtag ccgaacgcgc
    44461 ctcgccgcgc tggctggtgt tttcaatcac cacccaactg aaagtgctgt cgccatcaat
    44521 gccagcttca atcgccaccc aaaaatcagc ctctggatgt aaacggcggg catttgctac
    44581 ccgatttcgt gcgccagcgc gcgtttcctc actgccaaag ggctgttccg gtacaccgct
    44641 ctcgacggca acggatgcaa tatggcagga tccttcgccg aagatctcgt gaaatgcctg
    44701 cagaatggcc tgaattttag cgggattggt ggtcgcacag acaacttggt gcataatcag
    44761 cattactcag aaaattaacg ttacagcagt atacggaaaa aaagcatgtt acaggtatac
    44821 ctagtccgcc acggtgaaac gcagtggaac gccgagcgac gtattcaggg ccagtctgac
    44881 agcccgctga ccgccaaagg tgagcaacag gcgatgcagg tggcaacccg tgccaaagag
    44941 cttggcatta cgcatatcat cagtagcgat ttagggcgca cccggcgtac ggcggaaatc
    45001 atcgcccagg cctgcggctg tgacatcatc tttgattctc gcctgcgtga attaaacatg
    45061 ggtgtgctgg aaaaaagaca tatcgattct ctgaccgaag aagaagagaa ctggcgtcgg
    45121 cagctggtca atggcaccgt tgacgggcgt attcctgaag gcgagtcaat gcaggagctc
    45181 agcgatcgcg tcaatgcggc actggaatct tgccgggact taccgcaggg aagccgaccg
    45241 ctgctggtga gccacggtat tgcactggga tgcctggtga gtacgattct cggattacca
    45301 gcatgggcag agcgtcgctt acgtctgcgt aactgttcta tttcgcgcgt ggattatcag
    45361 gaaagcctgt ggctggcgtc tggctgggtc gttgaaaccg caggggacat ctcgcatctg
    45421 gacgcccctg cattagatga gctgcagcgt taacgacgga tcggaatcag tagttcacag
    45481 cgcagattaa ttgggcgatc tcccgctttg gcatcttctg ccgggtagta tcgctcaata
    45541 tcctgacctt tacggcgcgt caggttgagc attggcatgc acgtcccgta taccgtcagg
    45601 ataaactcct gcacgccggt tcccagacct tcataggtaa acatcacata ttcgccgccc
    45661 tgcagcatca ccggatgccc cgtcagtaca tagccatctg cctgatcctg ggctaacgcg
    45721 gtggtataga atacttcttg ttcgtcgtct ttatcctgac tcggacgcgt ttcattcagg
    45781 ccgtagagca ccggcggaat ggtcggcgcg ttgccgagaa aatcgtgcca gaactgataa
    45841 cgcatttcat ggcggaaatc agagatttgc tccagcgaac aggagtagct ctgggtaaca
    45901 ccaatcagcg gcgtatcttc cagggtgaca aatttgtgct ctggcatagt gaattcaccc
    45961 agacgtagcg gcgggcgaat accaaaggcg ctccattcag gagaacggcg gtaaagtgca
    46021 ggagtctggg caaactgctt cttgaatgcg cgggtaaatg tctgttgaga gtcgaagcgg
    46081 tattgcagcg cgatgtccag aatcggacgc gcagtcaggc gtagtgcgac cgccgatttc
    46141 gacaaacgac gagcacgaat atacgcgcca atagcatggc cagtgacatc tttaaacatt
    46201 ctctgtaagt gccacttgga ataacctgct ttcgccgcta cattgtcgag cgacaggggc
    46261 tgatccagat gaccttccag ccagattaaa aggtcgcgaa taatgccggc ctgatccata
    46321 aaatatcctc atcctttcaa caacgagcac ctgacatcag gtaattggat aatagcattt
    46381 tttgctgttt tagcattcag tgtttttttc ttagtagagt atgttttagg gcttgacgga
    46441 aataaaagta ttgagatttt gttcttaatc aatatgttat ttaccgtaac gaactaattg
    46501 ctcgtgtaat agataaaaat ggtaacaata tgaaatacaa gcatttgatc ctgtctttaa
    46561 gcctgataat gctggggcca ttggctcatg cagaagagat tggttcggtc gacaccgtat
    46621 ttaaaatgat cggcccggat cacaaaattg ttgtggaagc ctttgacgat cccgatgtga
    46681 aaaatgtcac ctgttatgtg agccgggcga aaaccggtgg tattaaaggg ggattgggcc
    46741 tggcagaaga tacctccgat gcggccattt cttgtcagca agtcgggccg attgaactgt
    46801 cggatcgtat taaaaacggc aaagctcagg gcgaggtagt attcaaaaaa cgcacgtccc
    46861 tggtctttaa gtcgttacag gtcgtgcgct tttatgacgc caaacgaaac gcgctcgctt
    46921 atctggctta ctccgacaaa gttgtagaag gttcgccgaa aaacgcgatt agcgcggttc
    46981 ctgtcatgcc gtggcggcaa taacagaggc gatttatgca acgggaaacg gtctggttag
    47041 tggaagatga gcaagggata gccgacacgc tggtctacat gttgcagcag gaaggttttg
    47101 ccgtcgaggt ctttgagcga ggcttgccgg tgctggataa agctcgccag caggtacccg
    47161 acgtcatgat tctcgatgtt ggtctgccgg atattagcgg ctttgaattg tgccgccagt
    47221 tactggcgct ccatccggcg ttacctgtac tgttcctgac ggcccgaagt gaagaggtcg
    47281 atcgcctgct tgggctggaa ataggcgctg acgactacgt ggctaaaccg ttttcacccc
    47341 gcgaagtgtg cgccagggtg cgcaccttac tgcgtcgggt gaagaagttc tcgacgccgt
    47401 ctcccgtcat ccgtattggt cattttgaat tgaatgaacc cgcggcgcag atcagctggt
    47461 ttgacacgcc attaacgctg acgcggtatg agtttttatt gctgaagacg ttactcaagt
    47521 cgccaggccg cgtctggtcc cgccagcaac tgatggatag cgtgtgggaa gatgcgcagg
    47581 acacctacga tcgcaccgtc gatacccaca ttaaaacgct gcgtgccaag ctgcgcgcca
    47641 tcaaccccga tctttcaccg attaatactc atcgcggcat gggatatagc ctgaggggcc
    47701 tgtaatgcgt atcggcatgc ggttgttgct gggctatttt ttactggtgg cggtggcggc
    47761 ctggttcgta ctggctattt ttgtcaaaga agttaaaccg ggcgtgcgaa gagcaacgga
    47821 ggggacgtta atcgacaccg caacgttgct ggcggagctg gcgcgtcccg atttgctctc
    47881 tggggaccca acgcatgggc aactggcgca ggcgtttaat cagctacaac atcgcccgtt
    47941 tcgcgccaat atcggtggca ttaacaaagt gcgcaatgaa tatcatgtct atatgaccga
    48001 tgcgcagggc aaagtattgt tcgattcggc aaataaagcc gttggacagg attattcgcg
    48061 ctggaatgac gtctggctaa cgttgcgtgg tcagtatggt gcgcgcagca cgttgcaaaa
    48121 tcctgccgat cccgaaagtt ctgtgatgta tgttgccgcg ccgattatgg acggctcgcg
    48181 gcttattggc gttttgagcg taggcaaacc gaacgcggcg atggctccgg tcattaagcg
    48241 cagcgagcgg cgaattttat gggccagcgc cattttgttg gggattgcac tggtgattgg
    48301 cgcatgcatg gtttggtgga tcaaccgctc cattgcccgg ctcactcgct atgctgattc
    48361 cgtcactgac aataagcccg ttcctcttcc cgatctcggt agtagcgagc tgcgtaaact
    48421 cgcgcaggcg ctggaaagta tgcgcgtgaa gctggaaggg aaaaactata ttgagcagta
    48481 tgtttacgcg ttaactcatg agctaaaaag cccactggcg gcgattcgtg gagcggcgga
    48541 aattttacgc gaaggtccgc cgccggaagt ggtggctcgt tttactgaca acattctgac
    48601 gcaaaatgcg cgtatgcagg cgctggtgga aacgttacta cgccaggcaa gactggagaa
    48661 ttgtcaggaa gtcgttctga ctgctgttga tgtggcggca ttattccgcc gcgtcagcga
    48721 agcgcgcacc gtgcagttgg cagaaaaaaa aatcactctg catgttacgc ccaccgaggt
    48781 taacgttgct gctgaaccgg cgttactgga gcaggcgctg ggaaatttac tggataacgc
    48841 catcgatttt acccccgaga gcggttgcat catgctaagc gccgaagtgg agcaggaaca
    48901 cgtcacgctt aaggtgctgg ataccggtag tggtattcct gactacgcgc tttcacgtat
    48961 ttttgaacgc ttttactctt tgccgcgtgc aaatgggcaa aaaagcagcg gtctggggtt
    49021 ggcgttcgtc agtgaggtcg cccgtttgtt taacggcgaa gtcacgctgc gcaacgtgca
    49081 agaaggtggc gtgctggctt cgcttcgact tcaccgtcac ttcacatagc ttcaaattct
    49141 tcccacatag ccttcgtatc ctgctgccat tgcaaaggag aagactatgt tgaaatcccc
    49201 cctgttctgg aaaatgacca ccctgtttgg tgcagtattg ctgttgttga ttccgataat
    49261 gctgattcgg caggtgattg tcgaacgtgc tgattaccgt agcgatgtgg aagatgcgat
    49321 tcgccaaagt accagcgggc cgcaaaaact cgttgggccg ctcatcgcta ttcctgtgac
    49381 cgagctttat acggtgcagg aagaggataa aaccgtggag cggaaacgaa gttttatcca
    49441 tttttggtta cctgagtcat tgatggttga tggcaatcag aacgtggaag aacgcaagat
    49501 agggatttat accggtcagg tctggcacag tgatttaacg ctaaaagccg atttcgatgt
    49561 ttcgcgtctt agcgaactca acgcgccaaa tatcacctta ggcaagccct ttattgtgat
    49621 tagcgtcggg gatgcgcgtg gtattggtgt ggtgaaagcg cctgaagtta acggaacggc
    49681 gctgaccatt gaacctggca ccggcttaga gcaaggcggg cagggcgtgc atatcccttt
    49741 acccgaaggg gactggcgga agcagaacct gaagctgaat atggcactga atttaagcgg
    49801 taccggcgat ctttctgtgg tgcctgccgg gcgtaatagc gaaatgacct taaccagcaa
    49861 ctggccgcat cccagttttt taggtgattt tctaccagcc aaacgggaag ttagcgagtc
    49921 aggttttcag gcgcaatggc aaagcagctg gtttgctaat aatctcggtg agcgttttgc
    49981 ttcaggcaat gataccggct gggaaaactt ccccgcgttt agcgtcgcag tgacgacgcc
    50041 agccgatcaa taccaattaa cggaccgggc gactaagtac gccattctgc tgattgcact
    50101 gacttttatg gcgttctttg tttttgaaac gctcaccgcg caacgtttac acccaatgca
    50161 atatttgctg gtggggcttt cattggtgat gttttatttg ctcttgctgg cgctttctga
    50221 acatatcggt tttaccgtgg catggataat cgccagtctg attggggcgt taatgaacgg
    50281 tatttatttg caggcggtat tgaaaggctg gcgcaatagc atgttgttta ccctcgcgct
    50341 gttgttgctg gatggtgtga tgtggggact gctcaactct gccgatagcg cgctgttgtt
    50401 gggaaccagt gtgctggtgg tggcgctggc cggcatgatg tttgtgaccc gtaatatcga
    50461 ctggtatgcg ttttcactgc cgaaaatgaa agccagtaaa gaagttacaa cggacgatga
    50521 attacgtatc tggaaataag gttgaaaaat aaaaacggcg ctaaaaagcg ccgttttttt
    50581 tgacggtggt aaagccgatt aatcttccag atcaccacag aagcgataac cttcaccgtg
    50641 aatggtggcg atgatttccg gcgtatccgg cgtagattcg aaatgtttac gaatacggcg
    50701 gatcgtcacg tctacagtac ggtcgtgcgg tttcagctca cggccggtca ttttcttcag
    50761 cagttcagca cgggactgaa ttttgcctgg gttttcacag aagtgaagca tggcgcggaa
    50821 ctcgctgcgc ggcagcttgt actgctcgcc atcagggccg atcaacgaac ggctgttgat
    50881 gtccagttcc caaccattga acttgtagct ttcaacgcta cgacgttctt cgctgacagt
    50941 acccagattc atggtacggg acagcaggtt gcgtgcacga atagtcagtt cacgtgggtt
    51001 gaacggtttg gtgatgtagt catctgcacc gatttcgagg ccgagaattt tatcgacttc
    51061 gttgtcacgg ccagtcagga acatcaacgc aacattcgcc tgctcgcgca gttcacgcgc
    51121 taacagaaga ccgttcttac ccggcagatt gatatccatg atcaccaggt tgatgtcata
    51181 ttcagagagg atctgatgca tttccgcgcc atctgtcgct tcgaaaacat catagccttc
    51241 cgcttcgaaa atacttttca acgtgttgcg tgttaccaac tcgtcttcaa cgataagaat
    51301 gtgcggggtc tgcatgtttg ctacctaaat tgccaactaa atcgaaacag gaagtacaaa
    51361 agtccctgac ctgcctgatg catgctgcaa attaacatga tcggcgtaac atgactaaag
    51421 tacgtaattg cgttcttgat gcactttcca tcaacgtcaa caacatcatt agcttggtcg
    51481 tgggtacttt ccctcaggac ccgacagtgt caaaaacggc tgtcatccta accattttaa
    51541 cagcaacata acaggctaag aggggccgga cacccaataa aactacgctt cgttgacata
    51601 tatcaagttc aattgtagca cgttaacagt ttgatgaaat catcgtatct aaatg
//
LOCUS       DAYPHN010000011        50849 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000011 DAYPHN010000000
VERSION     DAYPHN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.011"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..899)
                     /locus_tag="ACG0BW_000687"
     CDS             complement(<1..899)
                     /locus_tag="ACG0BW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFK7257692.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGL"
     gene            1235..2437
                     /gene="nupC"
                     /locus_tag="ACG0BW_000688"
     CDS             1235..2437
                     /gene="nupC"
                     /locus_tag="ACG0BW_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFK7257693.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2487..4676)
                     /gene="pdeA"
                     /locus_tag="ACG0BW_000689"
     CDS             complement(2487..4676)
                     /gene="pdeA"
                     /locus_tag="ACG0BW_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFK7257694.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(4884..4959)
                     /locus_tag="ACG0BW_000690"
     tRNA            complement(4884..4959)
                     /locus_tag="ACG0BW_000690"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4924..4926),aa:Ala,seq:ggc)
     gene            complement(4999..5074)
                     /locus_tag="ACG0BW_000691"
     tRNA            complement(4999..5074)
                     /locus_tag="ACG0BW_000691"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5039..5041),aa:Ala,seq:ggc)
     gene            5295..5654
                     /gene="yfeC"
                     /locus_tag="ACG0BW_000692"
     CDS             5295..5654
                     /gene="yfeC"
                     /locus_tag="ACG0BW_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFK7257695.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5656..6048
                     /gene="yfeD"
                     /locus_tag="ACG0BW_000693"
     CDS             5656..6048
                     /gene="yfeD"
                     /locus_tag="ACG0BW_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFK7257696.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(6099..7514)
                     /gene="gltX"
                     /locus_tag="ACG0BW_000694"
     CDS             complement(6099..7514)
                     /gene="gltX"
                     /locus_tag="ACG0BW_000694"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFK7257697.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLNLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            7773..7848
                     /locus_tag="ACG0BW_000695"
     tRNA            7773..7848
                     /locus_tag="ACG0BW_000695"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7806..7808,aa:Val,seq:tac)
     gene            7893..7968
                     /locus_tag="ACG0BW_000696"
     tRNA            7893..7968
                     /locus_tag="ACG0BW_000696"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7926..7928,aa:Val,seq:tac)
     gene            8015..8090
                     /locus_tag="ACG0BW_000697"
     tRNA            8015..8090
                     /locus_tag="ACG0BW_000697"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8048..8050,aa:Val,seq:tac)
     gene            8095..8170
                     /locus_tag="ACG0BW_000698"
     tRNA            8095..8170
                     /locus_tag="ACG0BW_000698"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8128..8130,aa:Lys,seq:ttt)
     gene            8290..8631
                     /locus_tag="ACG0BW_000699"
     CDS             8290..8631
                     /locus_tag="ACG0BW_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFK7257698.1"
                     /translation="MATISPTSIPSIQTQSSNRASEGSDVASQIARISQQIIKLTQQI
                     KEIADTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNASLPNP
                     VEKTTQIDIYI"
     gene            complement(8622..9548)
                     /gene="yfeR"
                     /locus_tag="ACG0BW_000700"
     CDS             complement(8622..9548)
                     /gene="yfeR"
                     /locus_tag="ACG0BW_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7257699.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRGQGRALMAGREGDPLYQI"
     gene            9638..10636
                     /gene="yfeH"
                     /locus_tag="ACG0BW_000701"
     CDS             9638..10636
                     /gene="yfeH"
                     /locus_tag="ACG0BW_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFK7257700.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKHQTEQLQAQQESRANKA"
     gene            complement(10633..10851)
                     /gene="ypeB"
                     /locus_tag="ACG0BW_000702"
     CDS             complement(10633..10851)
                     /gene="ypeB"
                     /locus_tag="ACG0BW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFK7257701.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(10853..12868)
                     /gene="ligA"
                     /locus_tag="ACG0BW_000703"
     CDS             complement(10853..12868)
                     /gene="ligA"
                     /locus_tag="ACG0BW_000703"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFK7257702.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDVEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQERLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(12939..13937)
                     /gene="zipA"
                     /locus_tag="ACG0BW_000704"
     CDS             complement(12939..13937)
                     /gene="zipA"
                     /locus_tag="ACG0BW_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFK7257703.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQPPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPKVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            14167..14928
                     /gene="cysZ"
                     /locus_tag="ACG0BW_000705"
     CDS             14167..14928
                     /gene="cysZ"
                     /locus_tag="ACG0BW_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFK7257704.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            15113..16084
                     /gene="cysK"
                     /locus_tag="ACG0BW_000706"
     CDS             15113..16084
                     /gene="cysK"
                     /locus_tag="ACG0BW_000706"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFK7257705.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            16468..16725
                     /gene="ptsH"
                     /locus_tag="ACG0BW_000707"
     CDS             16468..16725
                     /gene="ptsH"
                     /locus_tag="ACG0BW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFK7257706.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            16770..18497
                     /gene="ptsI"
                     /locus_tag="ACG0BW_000708"
     CDS             16770..18497
                     /gene="ptsI"
                     /locus_tag="ACG0BW_000708"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFK7257707.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            18538..19047
                     /gene="crr"
                     /locus_tag="ACG0BW_000709"
     CDS             18538..19047
                     /gene="crr"
                     /locus_tag="ACG0BW_000709"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFK7257708.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(19089..19940)
                     /gene="pdxK"
                     /locus_tag="ACG0BW_000710"
                     /gene_synonym="thiJ"
     CDS             complement(19089..19940)
                     /gene="pdxK"
                     /locus_tag="ACG0BW_000710"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFK7257709.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            20045..20413
                     /gene="yfeK"
                     /locus_tag="ACG0BW_000711"
     CDS             20045..20413
                     /gene="yfeK"
                     /locus_tag="ACG0BW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFK7257710.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(20416..21327)
                     /gene="cysM"
                     /locus_tag="ACG0BW_000712"
     CDS             complement(20416..21327)
                     /gene="cysM"
                     /locus_tag="ACG0BW_000712"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFK7257711.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQREAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(21461..22558)
                     /gene="cysA"
                     /locus_tag="ACG0BW_000713"
     CDS             complement(21461..22558)
                     /gene="cysA"
                     /locus_tag="ACG0BW_000713"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFK7257712.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(22548..23423)
                     /gene="cysW"
                     /locus_tag="ACG0BW_000714"
     CDS             complement(22548..23423)
                     /gene="cysW"
                     /locus_tag="ACG0BW_000714"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFK7257713.1"
                     /translation="MTEVTQLKRYGARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(23423..24256)
                     /gene="cysT"
                     /locus_tag="ACG0BW_000715"
     CDS             complement(23423..24256)
                     /gene="cysT"
                     /locus_tag="ACG0BW_000715"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFK7257714.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(24256..25272)
                     /gene="cysP"
                     /locus_tag="ACG0BW_000716"
     CDS             complement(24256..25272)
                     /gene="cysP"
                     /locus_tag="ACG0BW_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFK7257715.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(25443..26234)
                     /gene="ucpA"
                     /locus_tag="ACG0BW_000717"
     CDS             complement(25443..26234)
                     /gene="ucpA"
                     /locus_tag="ACG0BW_000717"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFK7257716.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYARSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(26363..27220)
                     /gene="murR"
                     /locus_tag="ACG0BW_000718"
     CDS             complement(26363..27220)
                     /gene="murR"
                     /locus_tag="ACG0BW_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFK7257717.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKLL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEAISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            27384..28280
                     /gene="murQ"
                     /locus_tag="ACG0BW_000719"
     CDS             27384..28280
                     /gene="murQ"
                     /locus_tag="ACG0BW_000719"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFK7257718.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALVACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            28284..29708
                     /gene="murP"
                     /locus_tag="ACG0BW_000720"
     CDS             28284..29708
                     /gene="murP"
                     /locus_tag="ACG0BW_000720"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFK7257719.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            29713..31014
                     /gene="pbp4b"
                     /locus_tag="ACG0BW_000721"
     CDS             29713..31014
                     /gene="pbp4b"
                     /locus_tag="ACG0BW_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFK7257720.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYTVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(31072..31971)
                     /gene="yfeX"
                     /locus_tag="ACG0BW_000722"
     CDS             complement(31072..31971)
                     /gene="yfeX"
                     /locus_tag="ACG0BW_000722"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFK7257721.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDANLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(32067..32642)
                     /gene="yfeY"
                     /locus_tag="ACG0BW_000723"
     CDS             complement(32067..32642)
                     /gene="yfeY"
                     /locus_tag="ACG0BW_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFK7257722.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQPIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     MISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(32703..33152)
                     /gene="yfeZ"
                     /locus_tag="ACG0BW_000724"
     CDS             complement(32703..33152)
                     /gene="yfeZ"
                     /locus_tag="ACG0BW_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFK7257723.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(33139..33564)
                     /gene="ypeA"
                     /locus_tag="ACG0BW_000725"
     CDS             complement(33139..33564)
                     /gene="ypeA"
                     /locus_tag="ACG0BW_000725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFK7257724.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            33778..34647
                     /gene="amiA"
                     /locus_tag="ACG0BW_000726"
     CDS             33778..34647
                     /gene="amiA"
                     /locus_tag="ACG0BW_000726"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFK7257725.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            34651..35550
                     /gene="hemF"
                     /locus_tag="ACG0BW_000727"
     CDS             34651..35550
                     /gene="hemF"
                     /locus_tag="ACG0BW_000727"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFK7257726.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGISLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYHPEDGSPEAALSEFIKVRDWV"
     gene            complement(35556..36608)
                     /gene="eutR"
                     /locus_tag="ACG0BW_000728"
     CDS             complement(35556..36608)
                     /gene="eutR"
                     /locus_tag="ACG0BW_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFK7257727.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(36654..37154)
                     /gene="eutK"
                     /locus_tag="ACG0BW_000729"
     CDS             complement(36654..37154)
                     /gene="eutK"
                     /locus_tag="ACG0BW_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFK7257728.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(37167..37826)
                     /gene="eutL"
                     /locus_tag="ACG0BW_000730"
     CDS             complement(37167..37826)
                     /gene="eutL"
                     /locus_tag="ACG0BW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFK7257729.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(37836..38723)
                     /gene="eutC"
                     /locus_tag="ACG0BW_000731"
     CDS             complement(37836..38723)
                     /gene="eutC"
                     /locus_tag="ACG0BW_000731"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFK7257730.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(38744..40105)
                     /gene="eutB"
                     /locus_tag="ACG0BW_000732"
     CDS             complement(38744..40105)
                     /gene="eutB"
                     /locus_tag="ACG0BW_000732"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFK7257731.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(40117..41520)
                     /gene="eutA"
                     /locus_tag="ACG0BW_000733"
     CDS             complement(40117..41520)
                     /gene="eutA"
                     /locus_tag="ACG0BW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFK7257732.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLIFEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPNSDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(41517..42743)
                     /gene="eutH"
                     /locus_tag="ACG0BW_000734"
     CDS             complement(41517..42743)
                     /gene="eutH"
                     /locus_tag="ACG0BW_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFK7257733.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(43259..44446)
                     /gene="eutG"
                     /locus_tag="ACG0BW_000735"
     CDS             complement(43259..44446)
                     /gene="eutG"
                     /locus_tag="ACG0BW_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFK7257734.1"
                     /translation="MQNELQAALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCEGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSERKQVLVHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(44436..45272)
                     /gene="eutJ"
                     /locus_tag="ACG0BW_000736"
     CDS             complement(44436..45272)
                     /gene="eutJ"
                     /locus_tag="ACG0BW_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFK7257735.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(45283..46686)
                     /gene="eutE"
                     /locus_tag="ACG0BW_000737"
     CDS             complement(45283..46686)
                     /gene="eutE"
                     /locus_tag="ACG0BW_000737"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFK7257736.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDEHGKGTVSRDWVGRDAAKIA
                     AAIGLTVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(46698..46985)
                     /gene="eutN"
                     /locus_tag="ACG0BW_000738"
     CDS             complement(46698..46985)
                     /gene="eutN"
                     /locus_tag="ACG0BW_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFK7257737.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(47092..47385)
                     /gene="eutM"
                     /locus_tag="ACG0BW_000739"
     CDS             complement(47092..47385)
                     /gene="eutM"
                     /locus_tag="ACG0BW_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFK7257738.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(47424..48440)
                     /gene="pta"
                     /locus_tag="ACG0BW_000740"
     CDS             complement(47424..48440)
                     /gene="pta"
                     /locus_tag="ACG0BW_000740"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFK7257739.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPQLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(48437..49240)
                     /gene="eutT"
                     /locus_tag="ACG0BW_000741"
     CDS             complement(48437..49240)
                     /gene="eutT"
                     /locus_tag="ACG0BW_000741"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFK7257740.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLAC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(49237..49938)
                     /gene="eutQ"
                     /locus_tag="ACG0BW_000742"
     CDS             complement(49237..49938)
                     /gene="eutQ"
                     /locus_tag="ACG0BW_000742"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFK7257741.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVAELLGFT
                     ITECDESTPVTASVPACVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(49913..50392)
                     /gene="eutP"
                     /locus_tag="ACG0BW_000743"
     CDS             complement(49913..50392)
                     /gene="eutP"
                     /locus_tag="ACG0BW_000743"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFK7257742.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGDYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVTATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(50405..50740)
                     /gene="eutS"
                     /locus_tag="ACG0BW_000744"
     CDS             complement(50405..50740)
                     /gene="eutS"
                     /locus_tag="ACG0BW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFK7257743.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKC"
BASE COUNT        11943 a        14121 c        12939 g        11846 t
ORIGIN      
        1 agtccggcag caaccaggct taatccaaag accgttgccg cagcatggct taacaacggt
       61 tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgacctga aaagtggaac
      121 gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatagt catggcgata
      181 gccacatccc atttggtggc ggaataacgt tgttgacgag aaccgccatg taaatgttga
      241 gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa cacgcctgct
      301 gccaggaaga ccgcttccga agtaggtaaa ctcgggatca ccattccttt acccagctgc
      361 gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac aaacaacagt
      421 aacccaccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat taaaatcagg
      481 aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag aatgagttta
      541 aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat aatttctgcc
      601 tgaacccaat agaaccacac tacgggacgc ggatagtgat cacgaatctg ctccgccaga
      661 tttttaccgg tggcaatccc cagtttggca gagaggatct gaatcagcat cgccatcagg
      721 ttggcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc ctgaatattg
      781 gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg tcccattaat
      841 gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata gttcgtcatc
      901 ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga gaatgattat
      961 caaattcatt taaatgggtt gtggtgattt ctctgataga ccaggattat gactacgaaa
     1021 agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca taactttcag
     1081 cttccataca caacatagca gaaatgtatg acagctcact atttttgaag cttgtcacag
     1141 gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat agaatgtgca
     1201 cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc attttgtact
     1261 ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca aaaaaattcg
     1321 tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa
     1381 ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgtttgaaa aactgctcgg
     1441 atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag gcctggcatt
     1501 cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg gtattctcca
     1561 gcacattcgc gtgttgccgg tgattatccg cgcaattggt ttcctgctct ccaaagtcaa
     1621 cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg gtcagtctga
     1681 aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta tgtacaccat
     1741 ggcagcaacg gcgatgtcca ccgtgtcgat gtccatcgtt ggtgcataca tgaccatgct
     1801 ggagccgaaa tacgtcgttg cggcgctggt actgaacatg ttcagcacct ttatcgtgct
     1861 gtcgctgatc aacccttacc gtgttgatgc cagtgaagaa aacattcaga tgtccaacct
     1921 acacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag gtttcaaagt
     1981 cgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg cgctgaacgc
     2041 actgtttgcc accgtgactg gctggtttgg ctacagcatc tccttccagg gcatccttgg
     2101 ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag cactgcaagt
     2161 gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga tggatctgca
     2221 gaaaattgcc tctacactct ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc
     2281 cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga
     2341 gcaaggtaac gtggtttctc gcttcggtct gaagctggtt tacggctcta ccctggtgag
     2401 tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac catacataaa aaagccgggg
     2461 ataattcccc ggctttttta cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa
     2521 tacccttgca gatattgcac cccgagctta tgcaatagcg cctgctgctg ttgcgtctcg
     2581 acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat tgatctcaca
     2641 atcatcgcat ccagcgtatt cgtgacgata tctttcacaa agacgccatc aattttgatg
     2701 atatcagcct gcaaacgctt taaccgttcg tagttggcat aaccggtgcc aaagtcatca
     2761 atcgcaatcc ggaaaccaaa cttatgcagt tgctcgatgt tgtacatgct gctttctgcg
     2821 ttagaaaacg cctgctcctc ggtgatctca agaatgaccg cctgcgggga gatgtgataa
     2881 cgcttaaaca gacgaataat ccgcccggca atattctttt gcagcagcgt gagcggcatt
     2941 aaattgactg aaaagcgcgg gccttttttg tcgcaaggat gtgttgccag ccacttcaac
     3001 agggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc aataaggggc
     3061 agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag gatctcatca
     3121 taaccttcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc accctgatct
     3181 aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc ggaaaccatc
     3241 tcctctctgc tatccagcgc cagcacatgg tgatgtgcgc aggattgctc cgccagccag
     3301 cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg cccccaggca
     3361 gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact attcaggatg
     3421 ttaaccatat gctggagtcg cccttccgtt tccggtccac tcagcactaa cagcagttca
     3481 ctacccggca attgatacaa cttttcgttt tcctgcatca acggcagcag cgtacggtaa
     3541 attgagcgga tacagtgaac gcgcatcatt aagccgtaat gacgactcat aaactcaaga
     3601 ttatcaatgc gcaggcagca aaaactcttg cccgcctctt gctccggcgc ttgctccaac
     3661 gcacgaaagt tgggtaggag cgttaacgga tcggtcagcg cctgcaaatg ccagcggcga
     3721 ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac gctaaaggaa
     3781 atcagcaccg cgagaataaa tgccagcgaa tattcggtta gtaccccttg caaaaagttc
     3841 tggttgtaat tcagcaggca aagcgttgaa acagcccagg tgagatttaa aaacggatag
     3901 cgaagcttac cgacaccgag ggtaaagatg ataaagaaaa caggtaccag gtaaccggca
     3961 ataaagtcat tctcatacgg tgtgcacatc agaagtagca gcacgctaag agctgccagc
     4021 cagcttaagg taaatgcgcg tttctctttg ctcaacgacg gagcgatatc cctgcgccac
     4081 aatatctgcg caaagtgggg acttacaatc atgcgagtga gatagtagaa aagcatgttg
     4141 taaatcagca cagcggtgaa aaggcttagt aaatcaacga ctgtgaaaat ggcatccgca
     4201 tcgccgaaaa aggtggatat ctttagcggg aaatcaaaaa agctccccac aagatacatg
     4261 ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga aaagacgttg ccagacatat
     4321 cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca
     4381 cacaacacaa caaacgtctg gctgccgagc atgaccgcgg tctgtaaaaa agagagatga
     4441 aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc gcgccagcca
     4501 aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa gatataactg
     4561 gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac cacggtgagc
     4621 gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac aaacatgagt
     4681 acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat tcgcatcgcg
     4741 aagcaaacag gggccagcct gttgccgatt tttcatccaa tgatgctttc accaggcggc
     4801 gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg
     4861 ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
     4921 catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt acgtaccaaa
     4981 tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc
     5041 catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt cgagttgacg
     5101 ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcaacg tgtagtttca
     5161 atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt
     5221 catgtcacgg tttcctgtaa agtggtgtta taaaatgaac tactaataga cccacataca
     5281 ttcagggaat tgttatgttc aaggagagga tgacgccaga tgaacttgcc agactgaccg
     5341 gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggaca acgtcaccaa
     5401 aacctggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat
     5461 atattcgcaa tgctgaacgt ccagaaggtc agggagaagc gcctgccctg tctggtgatg
     5521 cgccgcttga agtgttgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac
     5581 agtttacatc cttgcttctg cgggaaggga ttatcggatt attacaacgc ttagggattc
     5641 gcgatagcaa ataatatgaa aagattacgc aataaaatga ccaccgaaga actggctgaa
     5701 tgcctcggtg tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg
     5761 gaaaaatttc ctggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt
     5821 tgcgagttta ttcagaacac gccagccttc cataacacgc cgatgcttct ggaagccgaa
     5881 gaaccccttg ccgaatacgc accggggatt cgcactcctg cttatcggca aatcatcagc
     5941 gcaatagaca acatgactca cagtgagcag gaaaaagttg cgcaattttt gtcacgcgaa
     6001 ggaattcgta atttccttgc tcgtctcgac atagatgaat cagaataaat aaaaaacggc
     6061 aggggtatct ctcctgccgt ttatctttta tgcgctaatt actgctgatt ttcgcgttca
     6121 gcaataaaag ccagcgcttt gttgatacgc tcgatactgc gggtcttacc gattgcgtga
     6181 acggtaacat ccagcgccgg agactgcccc gcaccggtaa cggcgacacg cagcggcata
     6241 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
     6301 ttttcagctg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
     6361 cgcgctaccg gacgcagatg ttttttcgcg gcgtcggcat cgaactcagc aaaatcttcg
     6421 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc acccagtagt
     6481 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
     6541 cactgtaagt gagtagcaac atactccggc ggtagcgcgt taatgtagtg atggttcagc
     6601 catagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt cagggtgaag
     6661 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
     6721 accagatagt tcagcagtgc ttctggcaga taaccatcat cacgatactg cattacgctg
     6781 accgccccgt gacgtttgga cagtttttta ccgtcatcgc cgttgatcat agaaacgtgc
     6841 gcgtaaaccg gtaccggcgc tttcagtgct ttaagaatgt tgatctggcg tggcgtgttg
     6901 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
     6961 acacagaagt tataggttgg agaaccatcg gtacgacgga taatcagatc atccagctcc
     7021 tggttgctga attcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
     7081 gggttagcaa aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg
     7141 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
     7201 aggcgctctt tagagcaata gcatttataa gctgtgccct cttccagcat ctgatcgatc
     7261 accgcgttgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
     7321 ttcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
     7381 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
     7441 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
     7501 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
     7561 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
     7621 gcaaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
     7681 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
     7741 aactctccct ataatgcgac tccacacagc gggggtgatt agctcagctg ggagagcacc
     7801 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
     7861 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
     7921 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact ttctcgccag
     7981 ctaaatttct tgtaaaaatg tgaagtaccg aagtgggtga ttagctcagc tgggagagca
     8041 cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg
     8101 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
     8161 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatatgc ggtttgtaag
     8221 attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta cagtgacgag
     8281 gactgaacaa tggccactat ctctccaact tcaattcctt ccatacaaac acagagtagt
     8341 aacagggcat cagaaggtag cgatgttgct tcacaaattg cgcggataag tcagcaaatc
     8401 atcaaactca ctcaacaaat taaagagatt gcagacacta gcggcagtgc tgaagacaag
     8461 caaaaacagg ccgaattgat tcagcagcaa atcaccctac tggaaacaca actggcgcag
     8521 ttgcaaagac agcaggcaga aaaagcgcag gagaaagagc agcgtttgtc tctgaatgca
     8581 tcccttccaa atcctgttga aaaaacgact cagatcgata tctatatctg atacagcgga
     8641 tccccttccc gcccagccat tagagcacgg ccctggcctc gtaccacgtc ccataacgct
     8701 tgtgcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttccacc
     8761 accggcgtaa tgcgctttac caccagcggg ctaccttcag gtaacggcag cgccagtgcg
     8821 ggaagaatac taatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
     8881 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
     8941 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
     9001 acataatctt ccgcggccaa agcgctatcg cgatggcaaa gcaagaagaa aggctcggaa
     9061 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaaatcg
     9121 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggataaac
     9181 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     9241 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     9301 tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc
     9361 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     9421 ttttccagct ccttcacact gtgacttact gccgactgac tcaggccgat acgctctcct
     9481 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
     9541 taattcatct gttttattca tgaatggatg caataaatca attttatttc tcaaactgga
     9601 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
     9661 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
     9721 cgatttcgtc cccttctttg aaaatctgac cactgcagct attgccctgc tgttctttat
     9781 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtgggcact ggcgactgca
     9841 tttgtgggta atgtgcagca ccttcgtatt gtttccgatt ctgggcgtgc tgtttgcctg
     9901 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
     9961 ccccgctacc gtgcagtctg caatcgcctt tacttcaatg gcgggcggta acgtcgcggc
    10021 agcggtttgt tctgcgtcag catccagcct gttggggatt ttcctttcac cattgctggt
    10081 tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat
    10141 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
    10201 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
    10261 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
    10321 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgatcg tagttaacgt
    10381 ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg
    10441 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attcttttcc ccacatcggt
    10501 gatcggtatg atggtgctgc ctctgatgat tttccatcag atccaattga tggtctgtgc
    10561 ggtgctggcg cgtcgataca aacaccagac cgaacagtta caggcgcagc aggaaagccg
    10621 cgccaataaa gcttaaagag gacgcttcag gggctggacc agttgcgtca gcccctcagt
    10681 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt
    10741 gcgggcaaac cacagaagat actcctccgg caggtcgatt aagcgacgcc ctttgtattt
    10801 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
    10861 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg
    10921 ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc
    10981 ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc
    11041 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
    11101 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
    11161 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
    11221 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
    11281 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
    11341 gggcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccgatttcg
    11401 gccccatgcg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tccgccggag
    11461 tatggacata ttctttttca accagctgat cgatgatttt gtcgcccata ccgtcaacgt
    11521 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca
    11581 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
    11641 ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga
    11701 cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
    11761 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
    11821 cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
    11881 gaaactcgac gtcacgcaca aaggtcatct gttcctgcgc cgggaattta aacgctaccg
    11941 cccagcgcgg ggcacgcgcg acaaagccaa gccgctcctg ctgtgccagt gagttgacct
    12001 taatcaccac gccgtcgata tcaaaaccca gcgtcgggcg gtcttcttct actttgtggt
    12061 agaacgccag cacttcttcc gccgattcac aaagcgttac ccggtcgctg accggcaaac
    12121 cccactgttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
    12181 gaacaccgac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa
    12241 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaacactttc ccgcccgtac
    12301 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgtgg caggaacact tcaccacgta
    12361 cttccagacg cgccgggata ttctctccgt gtagcttcag cggaatggcg cgaatagtac
    12421 gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgagtcgcg gcactgacta
    12481 aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc
    12541 aggtgacttt ctcgttgctt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc
    12601 tttcttcatc aaaaacgtta tccagcgaca gcattggcac ttcatggcgg atctggctga
    12661 aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg
    12721 gatgtttggt ttccagctca cgcagttcgc gcatcagcct gtcgtattca acgtcgggaa
    12781 tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt
    12841 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
    12901 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact
    12961 tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg
    13021 tcaagaacaa caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
    13081 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta
    13141 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat
    13201 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
    13261 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
    13321 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
    13381 gccggttccg ctacaggctc aggctgtggt gccgctacag gctctgcagg ctggaaagcc
    13441 tgttgtgccg gttgcggtgc tgaatgcact ggctgcggcg ctgatgcgac ctttggcgaa
    13501 actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg cacgggttgc
    13561 tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc aggcggcggc
    13621 tggaccggtt gacgcggctg cgcagacgca taaggcggtt ggtactggtg ttgcggcgac
    13681 ggacgagcag cctcatgctc ctgggcgtta gccggggcat gattcacgcg gtgaacacga
    13741 acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt
    13801 gacttcattc gttttagtgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc
    13861 cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga
    13921 cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat
    13981 ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag
    14041 cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat
    14101 tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa ggagctcatc
    14161 ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa
    14221 ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc
    14281 aatattttgt tgatgggggg cgcattctgg tggctcttta cgcaactcga tgtctggatc
    14341 ccgactctca tgagttatgt tccggactgg ttacaatggc tgagctatct gttgtggcct
    14401 ctggcagtca tctccgtgct gttagtgttt ggctatttct tctcaacgat tgctaactgg
    14461 attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt gactggcgct
    14521 acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc
    14581 gaatggcaaa aatttgcctg gtatctgccg cgcgcaattg tattactaat tctttacttc
    14641 atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag cgcctggatg
    14701 ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg
    14761 cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagcctg
    14821 tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg tggtgcgacg
    14881 gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca atctaccggt
    14941 tattttgtaa accgcttgtg tgaaacaggg gtggcttatg ccgcccctta ttccatcttg
    15001 catgtcatta tttcccttct gcatatagat atgctaaatc cttacttccg catattctct
    15061 gagcgggtat gctagctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa
    15121 gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc tgaatcgcat
    15181 cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg
    15241 ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt
    15301 tgaactggtt gaaccaacca gcggtaatac cgggattgca ctggcctatg tcgctgccgc
    15361 tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct
    15421 gctgaaagcg ttaggtgcaa acctggtgct gaccgaaggt gctaaaggca tgaaaggcgc
    15481 aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca
    15541 attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg agatctggga
    15601 agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg gtacgctgac
    15661 cggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct ctgtcgccgt
    15721 tgagccaacc gattctccag ttatcgccca ggcgctggca ggtgaagaga ttaaacctgg
    15781 cccgcataaa attcagggta ttggcgctgg ttttatcccg gcgaacctcg atctcaagct
    15841 ggtcgataaa gtcattggca tcaccaatga agaagccatt tctaccgcgc gtcgtctgat
    15901 ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgctg cggcgttgaa
    15961 actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac cgtcatcggg
    16021 tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag aattgcaaca
    16081 gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt tcaggtgctt ttttgtggcc
    16141 tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca
    16201 gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt
    16261 ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta cgaaacgtca
    16321 gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa
    16381 aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac
    16441 actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg
    16501 aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct
    16561 gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag
    16621 actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga agacgagcag
    16681 aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt
    16741 taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta
    16801 tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa
    16861 tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat
    16921 cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag
    16981 ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag
    17041 ccctgattaa agataagcac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc
    17101 aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac
    17161 gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg
    17221 ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc
    17281 tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct
    17341 ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc
    17401 aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc
    17461 caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag
    17521 cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac caggtagaag
    17581 tgtgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag
    17641 gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg cccactgaag
    17701 aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag gcggttatcg
    17761 ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc ccgaaagaag
    17821 aaaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa gagatcctgc
    17881 gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc attatgttcc
    17941 cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa atctacaaac
    18001 aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc gtaatggtgg
    18061 aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat ttctttagta
    18121 tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat gatatgattt
    18181 cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa gttattgatg
    18241 cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc gatgaacgtg
    18301 ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc atttctatcc
    18361 cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg ttagcagagc
    18421 aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag ttcattgaag
    18481 aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg
    18541 ggtttgttcg ataaactgaa atctctggtt tccgacgaca agaaggatac cggaactatt
    18601 gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt
    18661 tttgcggaaa aaatcgttgg tgatggcatt gctatcaaac caactggaaa caaaatggtc
    18721 gcgcctgtag acggcaccat tggtaaaatc tttgaaacca accacgcgtt ctctatcgaa
    18781 tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc
    18841 gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt
    18901 gaattcgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc ggttgttatc
    18961 tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac cgtgggtgaa
    19021 accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc gcccatcggc
    19081 gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc atgctgctgg
    19141 gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc gtttaacgcc
    19201 ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt ccctttcagg
    19261 tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcagtgac caccaccacc
    19321 tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca ttttaatgtg
    19381 tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca atttttaccg
    19441 gtcaggattt ccagctcaaa aatattgggg gtaattccct gcgccagcgg cagtaaatat
    19501 tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat atcgccaatc
    19561 actggatcga ccatgatcaa taggtcagga tgatctttgc gtagcgcagt cagccactcg
    19621 gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc acgaagttgg
    19681 cgcaacgcat cacgctcctg aagcgcacgc aaatagccgc taaaccattc gtccggaatc
    19741 gcaccaccgt agaaagtgtc ataatgcggt gtattgctca gcaatacagt cggcacggca
    19801 aagacattca agccgttctg tttgatagca ggcacggcaa tgctgttgcc tacgctgccg
    19861 tacactacct gcgactgcac ggcgacgata tccgcctgca atgccctgct cttatcgtta
    19921 aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca tcgtattcca
    19981 accgaaactt tacctgattc tggcagtcaa atcggctatc acaaaacaag gataaggtaa
    20041 ttcaatgaag aaaatcattt gtctggtcat tacgctgcta atgacactcc ccgtttacgc
    20101 gaagttaact gcccatgaag aagcccgtat caacgccatg ctggagggat tagcacagaa
    20161 aaaggatttg atatttgtgc gtaacggcga tgaacacacc tgtgatgaag cggtttccca
    20221 cctgcgtctg aagctcggca acacgcgcaa ccgcattgac accgccgagc agtttattga
    20281 taaggtggcc tcgtcgtcat cgattaccgg gaagccgtat atcgtgaaga tgcccggtaa
    20341 aagcgatgag aacgcacaac cttttttaca tgcgctaatt gcgcagacgg ataaaacggt
    20401 gcctgcgcaa taatcttaaa tccccgcccc ctgactaaaa tgctcttccc caaacacccc
    20461 ggtggagagg tagcgatcgc cgcgatcgca gatgatcgcc acaaccaccg cgccggggtt
    20521 agcttttgcc acgcgcagtg ctccggcaac cgcgccgccg gagctgacgc cacagaatat
    20581 tccttcccgc actgccagtt cgcgcatggt gttttccgct tctcgctgat gaatatccag
    20641 cacctcatcc accagcgaag cgttgaaaat ccccggcaga tattccgcag gccagcggcg
    20701 aatgccggga atgctactgc cctcttccgg ctgcaggcca acaatggtca ccggtttgga
    20761 ttgttcgcgc ataaagcgtg aaacgccggt gatagtgccg gttgttccca tgctggagac
    20821 aaaatgggta atgcgcccgc cagtttgctg ccagatttcc ggcccggtgg tggtgtaatg
    20881 cgcataaggg ttatcgggat tattgaactg atcgagcagc tttccttcgc cacgattcgc
    20941 catctccagc gccagatcgc gcgcaccttc catgccctgc tctttggtga caagaatcag
    21001 ttccgcacca taagcacgca tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag
    21061 caatttcatg cgatagcctt taagcgcggc aatcattgcc agcgcaattc cggtgttacc
    21121 actggtggct tcgatcagca cgtcacccgg tttaatttcc ccgcgctttt ccgcctcgac
    21181 gatcatcgaa agtgccgcac gatcttttac cgaacctgcc gggttattgc cttccagttt
    21241 caaccagact tcactgccgt tattcggccc cattcgctgc aacttcacca gaggcgtatt
    21301 gccaattgtt tgttctaatg tactcacgat ctctgtccat acgtggtgtt gcctgatgcg
    21361 acgcttgcgc gtcttatcag gcctacaggt tacaaacctt gccattaaaa agcccggatc
    21421 gcgggaagcg cctccgggcg tttaacattc actcaaccta tcaggcgctt tgtgcgagag
    21481 caagttcctc atcgcgggtt tcaatacgct cgtcgccgtt atacagccgc gcatgttgca
    21541 gaccaacgaa taaacgctcg ccacgctgcg gggcatcgtc gccatgcatc acaaccgtca
    21601 gcggttcgtt gtaccacccc agcggctgca caactaattg ggtgtagtgg cctttcgggc
    21661 tggcttccag tacctgtacc ggcagcggcg aatcgaggct ggtacggcgg ctgatatcca
    21721 cttcccacgg acgaaggaag agatccaccg gcccctgata cgcaggtgta tagcccagcg
    21781 gccagcgatg tgcgccaaca tggaactgcc cgccgcgaat ggttccctgc aggcggttca
    21841 cttcgcccat aaattcgagt acaaaacggg tcgctggttc gcgccatacc tggtccggtg
    21901 catcagcctg ctcgatattg ccctggctca tcaccactac acgatcggct acttcggtcg
    21961 cttcttcctg gtcatgggtc acaaacacgc tggtgaattt cagctcttca tgtaactgac
    22021 gcagccagcg acgcagctct ttacgcacct gcgcatccag cgcgccaaac ggttcatcaa
    22081 gcagcagaat ttgcggttcc acagcgagcg cacgcgccag cgccacgcgc tgtttctgcc
    22141 cgccggaaag ctgtgccgga taacgatccg ccagatgcgc aagctgcacc atctccagca
    22201 attttgtcac tttcgctttg atcgccgccg cattcgggcg ttcacgccgc ggcaacaccg
    22261 tcaggccaaa agcgatattg tcgaacaccg tcatatgacg gaacagcgca taatgctgga
    22321 acacgaaacc gactttacgg tcccgcgcgt gcagacggct cacgtcggta ccgtggaagc
    22381 gaatatgccc gctggtttga tgctccagcc cggcgataat ccgcagcagc gtggttttcc
    22441 cggaaccgga cggccccagc aacgcgacca tctgaccaga aggaatatcc agcgagatat
    22501 cgttcagcac ctgggtgcga ccaaaagact tcttaatatt ggcaatctca atgctcatga
    22561 tgttcctcct gctgcgcgcg tttttcctga ttctccaggc gccactgcaa catactcttt
    22621 aaaaacaggg tgataatcgc catcagcgtc aacagcgcag cggcggtaaa ggagccgacg
    22681 gtgttgtagt cctgctccag caattcaatc tgtagcggca gcgaaagtgt ttcgccgcga
    22741 atcgagccgg aaaccaccga caccgcacca aactcgccaa tcgcgcgggc gttggtcagc
    22801 accacgccat aaagcagcgc ccagcggatg ttcggcagcg tgacgcgacg gaacatctgc
    22861 cagccagatg cgcccagcaa aatcgccgct tcgtcttcct ggctgccctg gcttaacatc
    22921 accggcacca gctcacgcac cacaaacgga cacgtcacga agatggtgac cagcaccatt
    22981 cccggccagg agaacataat ttgcagatta tgttcatcca gccagccacc gagcgggccg
    23041 ttagagccgt aaaacagcag atacaccaga ccggcaacca ccggcgatac ggcaaacgga
    23101 atgtccagta gcgtcagcag taactggcgt ccggggaagt taaagcgcgt caccagccac
    23161 gccagcagaa tgccgaacac caggtttacc ggtacggcaa tcaacgcaat cattaccgtc
    23221 agccagatgg cgtgcagcat gtccggatcg gccagattct gtaaaactgg catcagccct
    23281 ttgctgaacg cctgcacgaa gatgtaaatc atcggcacca gcaggatgaa cgccgaaacc
    23341 agcatcccga taccaatcag aaaccacttg ccccagttaa tcgggcgcgc gccataacgc
    23401 ttcaattggg taacttccgt cattaatgac ctaccacacg ccgaccaaag cgactttgca
    23461 gagtgttaat tgagaacaac agcagcaggg atgccgcgag gatcaccgaa gcaatcgcgc
    23521 tcgccgccgg gtaatcaaac tcctgtaagc gaacaaaaat catcagcgac gtcacttccg
    23581 tcttccaggc aatgttcccg gcgatgaaaa tcaccgcgcc aaattcacca agactacggg
    23641 taaacgacag cgccacgccc gccaccagcg ccggagaaag ttctggcagc accactttgc
    23701 agaaactctg ccagcgcgtt gcgccgagcg tttctgccgc ttcttcatat tccgggccta
    23761 actcttccag caccggttgc acggtacgca ccacaaacgg aatgctggta aaggccatcg
    23821 ccaccgcaat ccccagccag gtataggtaa ctttgatatc aaacttcgcc agccattcac
    23881 cgtaaaaacc gttcactgaa aagagcgagg ccagcgtcaa accggcgact gccgttggca
    23941 gcgcaaaggg taaatccatc agcgcatcaa gcagcgtgcg gcccggaaag cgatagcggg
    24001 ttaaaatcca cgccattagc aaaccgaaaa cgccgttaaa aattgatgcc acaaacgctg
    24061 acagcagcgt cactttgtag gccgcgacca cttgtgggtt ggtgatcacc tcccagtact
    24121 gcgcccagct catctgagcc agctgcatca ccagcgcgga gagcggcagc agcaaaataa
    24181 ggcacacaaa cagcagactg gtgccgaggc ttaaggtaaa gcccggcagc acgcgtcttg
    24241 atgaaactgc aaacattaat tacgccccgc cgctaacagc ttgtctaact cgccgccgct
    24301 ggtgaagtgg gttttcatca cttccggcca ggagccaaat ttgtcttcca cgcggaacag
    24361 ttcggtctgc gggaatttgt ctttcagttt gtccatgact tccgggttat ttacgcggta
    24421 gtaatagtcg gtgatgatgg tttgcgcctg cgggctgtag agccagttca ggtaggcttt
    24481 tgctgctttt tccgtaccgt tggcctgcac gtttttatcg acccacgcca ccgggaattc
    24541 cgccagaatg ttggttttcg gaatcaccac ttcaaagccc tgcgcttcat actgtttgcg
    24601 gatgttgttc acttccgact cgaagctgat cagcacatcg cccaggccgc gctcggcaaa
    24661 agtggtggtc gcgccacgac ctccagtatc gaacacttca acgtttttca ggaactgggt
    24721 cataaactgt tcggttttgg ctttgtcgcc accgtcagct ttatccgctg cgccccatgc
    24781 cgccagatag gtgtaacgtg cgttacccga cgttttcggg ttcgggaaaa tcagcttcac
    24841 gtcggagcgc accagatcgt tccagtcgtg gatatttttc gggttgccct tacgcaccag
    24901 gaaacccatg gtggagtaga acggcgagct gttattcggc aggcgcgttt gccagtcggc
    24961 cgggatcagc ttgcctttgt cgtgcaggat ttgtacgtca gtcacctggt tataagtgac
    25021 aacgtcggct tttaagccct gcaaaatcgc caacgcctgt tttgatgacc cggcatgaga
    25081 ttgttttatc gtcagtttgt cgccgccgtt atctttggcc cattgttgct caaacggcgg
    25141 gttcagggcg gcaaacagct cgcgggagac gtcataagaa ctgttcagca gttccgttgc
    25201 ctgtacatgg cccgccagca gcagagaagc gaccagcgcg agtgagttct ttttcagtaa
    25261 gttaacggcc attgcgcacc cttataaatt taatgacttt ctaatagcca tcatatttat
    25321 aacggacgtg aaaggagtaa cggttttata taccgtttgg tgatttggaa gttgaaaagg
    25381 gaataagaac cgggttcaag acgaaatcct ccccacaaat gcagggagga aacagaggtg
    25441 aatcagatgc cgacgctaac cgtctccggc agtgtgctgc cgccatcaat cacattctgt
    25501 gtaccggtta aataacttga ttcatccgat gcgaggaagg ctgccagttc gccgacttcc
    25561 agcggatcgg cgaggcgacg catcgggatt gcttttgcca tttcagtcag caccgattct
    25621 ggatcttccg ggttcgactg gcgggcaatg ctttccgcca ttggcgtgcg tacgtatccc
    25681 gggcagatgg cgttaacgcg aataccagac cgcgcgtact ccaccgccag cgatttagtc
    25741 aggccaacaa tcgccgcttt cgttaaggca tacgccgttt cacccggatc ggccaccata
    25801 tcgccagtga ctgaagacat catcacaatg cgaccatctt tgcgggcaat catctccggc
    25861 agcaccgcct tcgtgacgtt ccatacacct ttaatattga tatcaatatg gaaatcgcga
    25921 tcttcatcgc tcatatcgag gaaactgccc agacgacaaa cgcctgcgtt attcaccagg
    25981 atatcaatgc gcccttcttt ttccttcgcg cgcttgatag ctgcggcgac cgacgccggg
    26041 tcacgcacat cggcgacaac cgccgtacag cgatgaccac gaccacacag ttcgtccgcc
    26101 agcttttcga tctcagggga gatatccagc aagattaggt ttgcgccatg acgcgcaaaa
    26161 gttctggcaa ttccttcgcc aattccctgc aatgcgcccg taatcagtgc tgtcttgccc
    26221 gtgagtttac ccattttaat gctctccttg ttaatacaga gcgttacact ccattccctc
    26281 tgaaaataca ggataaacaa aatattgctg cggatcagct cactaattca ggaaaatctc
    26341 aattcagtcg ctgctttatg catcatttca ggcgttgcgt tagctcactg ctgcgctcaa
    26401 tcattttcaa tgactccaca tcattgagtt gcacaaggcc aacaaacagt aaatcggtaa
    26461 cagaattttg cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa
    26521 cggtatcgag cgtgaaatgc gccagccgcc gcagcggaga atcggttaac gaggtaatgg
    26581 caatcaccgt tgccccctgc tttcgtgctg cctcggcgca caagacaatc tcttttttac
    26641 tgccgctgta agagatagcg atttgtacat cgcctttttt tagcgcctga gaaacggtgg
    26701 cttgtacgtg cgtgtcggct tcacaggcca cgcgatagcc aattttcatc agtttgaacg
    26761 acagatcgcg ccccaccagc gccgacccgc ccaggcccgt tatctggata aacggtgctt
    26821 tactgatggc ctcaatgatt ttttgcagcc gcgcgtagtc gaacaacgcg caggtttgct
    26881 ccagcgccag ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac
    26941 tggtaatcga actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt
    27001 attcgccaat taacgccata cgcaattcag taaatccttg cgcgccgagc ttctgggcaa
    27061 acttcacaat actcgactgg ctgatgccga gcagtttcgc catcttgcgc gaagagacag
    27121 atttcagctc actcacgtta gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa
    27181 attcactttc cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg
    27241 cagcaaaaat tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa
    27301 tgattccttt aagtctgtca attcgggata tatgattcca tacatctgca attatttgat
    27361 cgtaaatagt aaggtcacca ccgatgcaac ttgaaaagat gattaccgaa ggctcgaacg
    27421 ccgcctcggc tgaaattgac cgcgtatcaa cgctggaaat gtgccggatt atcaacgatg
    27481 aagataaaac cgtgccgctt gccgttgagc gcgtactgcc ggatatcgcc gcggcgatcg
    27541 atgttatcca cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat
    27601 ccggtcgtct ggggattctg gatgccagcg aatgtccgcc cacctacggc gttaaaccgg
    27661 gcctggtagt tggtttgatt gctggcggcg aatatgccat tcagcacgcg gtggaaggcg
    27721 cggaagatag ccgggaaggc ggtgttaatg atctgaaaaa tatcggctta accgcgcagg
    27781 atgtggtggt tggtattgcg gccagcggac gcacgccgta tgtgattgcc ggactggaat
    27841 acgcacgcca gctcggctgc cgcacagtgg gaatttcctg taatccgggg agcgccgttt
    27901 caaccaccgc agagtttgcc attacgccag ttgttggggc cgaagtggtc actggttctt
    27961 cgcgaatgaa agcaggcacg gcgcagaaac tggtgctcaa tatgctttcc accgggctga
    28021 tgattaaatc cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaatgaaa
    28081 aactgcatgt acgccaggtc aatatcgtca aaaacgctac cggatgtagc gcagagcaag
    28141 cggaagcggc attggttgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga
    28201 aaaatctcga tgccgcagaa gctaaaaaac gccttgatca acacggcggc tttattcgtc
    28261 aggttttaga caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca
    28321 ttcttacccg tgtcggtgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc
    28381 tgcgtctggg tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg
    28441 tgaagggcgt cattttgacc agcgaccagg tgcaggtcgt ttttgggcca ggtaaagcgc
    28501 atcgcgccgc aaaagcgatg agcgagctgc tgggagaagc accggtacag gatgccgcag
    28561 aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat
    28621 ttctcgccaa attcgccacc atcttcacgc cgctgatccc cggttttatt gctgcgggtc
    28681 tgttgctggg gatagcgacg ttaatcgcca cggtgatgca cgttccggca gatgctcagg
    28741 gaacgctgcc cgacgcgctg aattttatga aggtgttcag caaaggcttg ttcaccttcc
    28801 tggtaattct ggtgggctat aacgcggccc aggcgttcgg cggcacgggc gtcaatggcg
    28861 caattatcgc cgcgctcttt ttgctcggtt ataaccctgc tgccaccact ggttactacg
    28921 ccggatttca cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgt
    28981 tgattgccgc gtgggcctgc gcccgcatcg aaggcatggt gcgccgcttt atgccggacg
    29041 atctcgacat gctgttgacc tcgttaatca ccctgctgat caccgctacg ctggcctacc
    29101 tgatcattat gccgctgggc ggctggctgt ttgaaggcat gtcgtggctg tttatgcacc
    29161 tgaacagtaa tccgctcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtgt
    29221 ttggcgtgca tcagggcttt attcctgttt acctcgcgtt aatggacagt cagggattca
    29281 acagcttatt tcccatcctt tcgatggcag gcgcgggcca ggtgggcgcg gcgctggcac
    29341 tctactggcg ggcgcaaccg cacagtgcat tacgcagtca ggtacgcggg gcgattattc
    29401 ccggcctgct gggcgttggc gaaccgttga tttatggcgt caccctgccc cgcatgaaac
    29461 cgtttattac cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct
    29521 ggtggggtct gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc
    29581 cgctgatgac ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc
    29641 tggtggcatg ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc
    29701 tggactgata tcatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc
    29761 gtcagcgccg caaaatacac tgttctgaca gaaagttcgc cagagaaagc agggttcaac
    29821 gtcgaacggc ttaaccagat ggatcgctgg attagccagc aagtggacgc cggttatccc
    29881 ggcgtaaacc tgctgattat aaaagataat cagattgtgt atcgcaaagc ctggggagcg
    29941 gcgaaaaagt acgatggcag cgtattgatg gaacagcctg tcaaagccac caccgggacg
    30001 ctgtatgacc tggcttccaa taccaagatg tacgccacta acttcgccct gcaaaagctg
    30061 atgagcgaag gcaaactgca tcctgacgat cggattgcga aatatattcc agggtttgcc
    30121 gacagcccga acgatgcgat caaaggcaaa aacacgctgc ggatttctga cctgctgcat
    30181 cacagcggcg gtttcccggc agatccgcaa tacccgaata aagcggtcgc gggcgcgtta
    30241 tattcccagg ataaaggcca gacgctggag atgatcaagc gcacgccgct ggagtatcag
    30301 cccggcagca agcatatcta cagcgatgtc gattatatgc tgcttggatt tatcgtcgag
    30361 tcggttaccg gtcagccact tgaccgctat gttgaagagt cgatttatcg cccgctcggc
    30421 ctgacgcata cggtgtttaa tccgctactg aaaggcttta aaccgcaaca aattgccgcc
    30481 acggaactga acggcaatac ccgcgatggc gtgattcact tcccgaatat ccgcacctcc
    30541 actctctggg gtcaggtgca cgatgaaaaa gcattctatt cgatgggcgg cgtttccggg
    30601 cacgcaggtt tgttttccaa taccggcgat attgcggtgt taatgcaaac gatgctgaac
    30661 ggcggcggct atggcgatgt gcagctgttc agtgcggaaa cggtgaagat gttcaccacc
    30721 agctctaagg aagatgccac ttttggcctc ggctggcgcg tgaatggtaa tgccactatg
    30781 acgccgacgt ttggcacgct ggcaagcccg caaacctacg gtcacactgg ctggaccggg
    30841 acggtgaccg ttatcgatcc ggtgaatcat atggcgattg tgatgttaag caacaagcca
    30901 cattcgccag ttgccgatcc gcaaaagaat cccaatatgt ttgaaagcgg tcagttgccg
    30961 attgcaactt atggttgggt ggttgatcag gtgtatgcgg cgttaaagca gtaataaaaa
    31021 ggccagtcag ttgtgatact ggctggcctt taatcatcga atcagaaacg attacaacgc
    31081 catcaacttg tccagcgacg gcgcgaagta atagccgcca gtgaccggtt tggtgaaacg
    31141 caacatcgca tcacgtttgc catcggtatc gccaaacatg ctcagcagtt gctgctcaat
    31201 gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac tggcggtgcc
    31261 gtacggcagg ctctggcgaa caatcttcag ccctttgcca tcttctttca gatcaacgcg
    31321 ggtcaggtga gaggtttccg gacgttcgtc gccgtcaatc tcttcgttgg cctctttagt
    31381 gcgcccgatc atcatctctt gatcgtgaac gctcatacgg ttgagttgct tcaggttgtg
    31441 ctcccaacgc tgcacaaaca catagctgcc gcccgcatcc acgccgtctt tgataaccgc
    31501 cacttcgcgg cgcgtctctt cacccgccgg gttttctgta ccgtcaacga agccgctcag
    31561 gtcacgctct tcaacccaac ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc
    31621 aaaggcttcc atcgccgcct gagcgacaga gaagtttacg tcgtgacgca gagaaagaat
    31681 gtggatcaac acatcgaact gggtcgtcgg cgcaaggcct ttaccgtagc ccggaaaatc
    31741 tttcagctct tcggccccaa cgccgccgct cagagcgcgc caggtgttgt taccaaaggc
    31801 aaccaccgca ccaagattcg catccgggaa tttcgcttca aaagttgcca gtttgtcggc
    31861 aaatgtttta ctggccgcac gcagggcgtc aacttcccct ttcacgttgg cttcgatcca
    31921 aatcgccgcg cggcaatgtt ctggcaaaat gccactctga acctgagaca ttgttcctcc
    31981 tgaaaataag aatgccacgc aatccgtggc attgttgggc gctattttac gtgttttttc
    32041 gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga taattttact gactttccag
    32101 tttttcaggg tatcgtccga aggcattaac ccttcaggac cactccattc cccgctgaac
    32161 tggtagctaa tatgttgact gccttcggct ttacattcga cggcacgatt atcatcacca
    32221 tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt
    32281 ttaacaccgg tgtcagcagg aatatcgcta tccagcacat caatgcggct gatcatgcct
    32341 tgatcaccgt taatcaccat cgccacgttg tcgcctttca tcacttcaaa aaagcgcacc
    32401 acgttgccgt tcgcggtttt cattccgctg cgcaggcgat aatcgccatc aagcgcatcg
    32461 gcaatgggtt gttcttgcag tggtgtggac gccgttaatt cacccacgcc ctgctcgctc
    32521 actttggtgg acgacccaaa ccagttccac gggttagcgg cagaccagtt aactgacgac
    32581 atcgtggaac agccggtcag catcaacggc atagcgcata acattaaacg cagcgatttc
    32641 atgtcacttc ctttggttat tcaataacgt tgcttggagt gcaaattcac caaaaagtgc
    32701 cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc gattggtcag cagccagatt
    32761 aacgccacaa tatccgccac taccagcgcc agaccgatac cgctaacgga ttcaccgttc
    32821 agccacagcc acggttgcca gcaaagtaaa accacttgcg ccaacagcaa cagaaaatag
    32881 agcacatgcc agatacgggg aaatgaagcc cgccgaccac tcagcaaaaa tgccagcacc
    32941 gccggaatac cgggaatcaa ccccagccag aaattatcgt gatcgggata aaacagattt
    33001 agcagcgcag taccctgctc gcgcgacgca ccggcaatga caaacagcac ccaggttcgc
    33061 gcctgaagca atagcacaag ccagaagagc aagggtaaac gcaggcgacc gtgcgcatca
    33121 taatggacag gatgaaactc agtactcttc atcttcaatc aaacgcttac ccagactcag
    33181 cacgtcggcg tgttcatatc ccaggcgttc atacattccg agcaccatgt cgttatcttc
    33241 cggcacgttg atctgaattt tcggacagcc gcgagcaatc agctttttct ccagccgatt
    33301 aagcaacgca ttagcaatcc cacgcccacg aaactctgga tgcacgccaa gataataagc
    33361 agacccgcga tgcccgtcat aaccgcccat caccgttccg accacttcac cgttcacctc
    33421 agcgaccaga aacaaactga cgtcatggtt catcttacgc tcgatgtcca tttccggatc
    33481 gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc
    33541 ctggcgaaat acgcgtatct ccatggtatt cgttaccttt ttgcgggtta aaaggctgat
    33601 tatggcgtga acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa aagtggcata
    33661 atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat tctcatcagc aaacggtcgt
    33721 aacggattac gcgatacgat ataacatctg gaactttatt attacaactc aggccgtatg
    33781 agcactttta aaccactaaa aacactcact tcgcgccgcc aggtgctgaa agcaggactt
    33841 gcagcgctga cgttgtcagg aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc
    33901 agcaacggac acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc
    33961 gatccaggcc acggcgggat tgataccgga gcgatcgggc gcaacggttc gaaagaaaaa
    34021 catgtggtgc tggcgattgc taaaaacgtc cgttccattt tgcgtaatca tggcattgat
    34081 gcgcgtttaa cgcgttctgg cgatacgttt atcccactat acgatcgcgt tgaaatcgcc
    34141 cataaacatg gcgcagatct gtttatgtca attcatgccg acggctttac caacccgaaa
    34201 gctgccggtg cttcggtatt tgccctctct aaccgtgggg caagtagcgc aatggcgaaa
    34261 tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag
    34321 gatcacctat tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat taaaaacagc
    34381 ctgacgctcg gctcgcatat tctgaaaaag attaagccgg tgcataaact gcacagccgc
    34441 aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg
    34501 gaaacctcgt ttatcaccaa cccagaagaa gagcggctgt taggcactgc ggcgtttcgc
    34561 cagaaaatcg ccacagcgat tgccgaaggc gtgatcagtt atttccactg gtttgacaac
    34621 cagaaagcac actcgagaaa gcggtaagtg atgaaacccg acgcacacca ggttaaacag
    34681 ttcctgctca accttcagga tacaatttgt cagcagctga gcgccgtcga tggcgcagag
    34741 tttgtcgaag atagctggca gcgcgaagct ggcggcggcg ggcgtagtcg ggttttgcgt
    34801 aatggtggtg ttttcgaaca ggcgggtgtc aacttttcac atgtccacgg tgaggcgatg
    34861 cctgcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc
    34921 atttcactgg tagtgcatcc gcataatccg tatgttccca ccagccacgc gaatgtgcgc
    34981 ttttttattg ccgaaaaacc gggtgccgaa cccgtctggt ggtttggtgg cggcttcgat
    35041 ttaacccctt tctatggatt tgaagaagac gccattcact ggcaccgcac cgcccgtgac
    35101 ctgtgccagc catttggtga agacgtttat ccccgttaca aaaagtggtg cgacgaatac
    35161 ttctacctca aacatcgcaa cgaacagcgc ggcattggcg ggctgttctt tgatgatctg
    35221 aacaccccag atttcgacca ctgttttgcc tttatgcagg cggtaggcaa aggctacacc
    35281 aacgcttatt taccaattgt agagcggcgt aaagcgatgg cctacggcga gcgcgagcgc
    35341 aatttccagc tttatcggcg cggtcgctat gtcgaattca acctggtatg ggatcgcggc
    35401 acgctgtttg gcctgcaaac cggcgggcgc accgagtcga tcctgatgtc aatgccgccg
    35461 ctggtacgct gggagtacga ttatcatcca gaagatggca gtccggaagc ggcgttaagt
    35521 gagtttatta aggtcaggga ttgggtgtaa ctccctcacc cccattcccg catccgctga
    35581 tgcaacgtca acgacggctt ctcggcaaac agctgctggt aatccgtggc aaattgcccc
    35641 agatgccaga atccccactg catggcggcg tcttttaccg ttgtgctttg cgaccaaggg
    35701 cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt tcaaccacgc atttgggcca
    35761 atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc ggctgacatg cagttgatta
    35821 cacaagtcca gcaccgtcac cggctcagac atattttcca gcacatattc acgggcgcgg
    35881 gaaagtaatc tacggtaact ctgatgactg atgctttccg ccgtcaccat tggttgcgct
    35941 tcttccagca tcgcccccat cgccattagc aaattatccc ccagcacttt tcgcactgca
    36001 ggctgatgga gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc
    36061 cacagcgcgg ctttatgctg ttctttcact tccagcgccg actggctacg cagcatatgt
    36121 aatacccgat ccggattatg caaaaagtta gcctgacggg tgatgacatc ttcagaaagc
    36181 actacgccca ggatcgtgta atcgtccggc gtacttaatt caaactcggt tccaccaggg
    36241 cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga taaatccctg ctcaccgcgc
    36301 gtcgccggaa tgccaaacca gaacgagttc ggccagacca ggcacgactg acgcagcgcc
    36361 agaccggtgt attcacgaaa aacctgaata tcatcgagta gaatttccgt aaattcacca
    36421 tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat cgtcaaagca
    36481 tgttcataga catccgttgt ctgtcgttga tgaacattat ccacttcgac cttcggcgtg
    36541 agcttcaggt tttcgggtaa gggttcatga taaagatggt gcaaattggc tgtacgggtc
    36601 tttttcatga tgttaatgcc gggtgttgta ggacacccgg cacctccgac aggttaatgg
    36661 ggcttgagac gataacgact actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg
    36721 agcgcatttc tggctttttc gagcggccag ccaaagtggg ctgccacttc tcctgccgtc
    36781 attccctgac gtactgatgt taacagcgcc aacaactcgt cagcagattc cgcgacgata
    36841 actggcgcgt ctggttcctt tacgggttgc ttcggctggc ggttaaagcc agtaaccagc
    36901 cactgggtgt catcgtctgg ccgaccgatc tccttacggc tgatgacacg cccggtacgc
    36961 atcgcggcag cacaacctgc gtccagcgcc gcacgacacg ccgccagatc gccttccacc
    37021 accagcgtta ggcgaccagg gtcaagcact tcatgactga gcagacgaac gttagctgct
    37081 ttgagcatgg catccgctgc atctatcgcg gcgaccatgc cgtccacttc cagcaatccc
    37141 agtgcattga tcatcggcaa cctccgttac gcacgctgga ttggattacg cgcgatttcc
    37201 agcactgcat cggtaaaggc gttacaggct gctttacacg cggcctggct accggttaaa
    37261 aatgctgccg agtagttggt ttcagacggc ggcgggacat aggtcaccag ctgtacgtcg
    37321 gcagatttca gggctgcatc aatgccgtag gtcgcttcca gcggcggtgc caccagatac
    37381 gccatcggat cgccaagcgt gatcccggcg gttgatgaga gataagaacc ggtacgcgaa
    37441 actacatgtg ccaggaatgc cgtatcttgc gcatcattag cccactggaa agccgcgcca
    37501 ttttcaatat gcgcaaccat cgcatccaga ccagcacgca cttccgccgg gtttggccca
    37561 ccgagcataa tcagcacttc accggcagtc ggtgacggtc cgtgtgcagc tcccgcatac
    37621 agcgagcggc catacaccac ttcgaccatc gcctgcttgg tcgcttcgtc agccgcaatg
    37681 taggttacgt catcggagtc ggcagaaatc agcccaaggc tacgaatatg cggcggcaat
    37741 ttcagttcac gcgcaaattc ggcgttaacg gaggcaatca cccgcatggc ggttaccgac
    37801 ggtcgaatca aatctaaagc tggcatgatg cctccttatc gggtcatgtt gatgccggat
    37861 gctttctgct ccagcatacg tttcgccaaa tccacaatga cggctgcggc ttcaaccggc
    37921 ggcgtgccgc cctggtgaat gttagagata caggtgcgat cggcttcaac ggtggttgcc
    37981 atacgcggcg agtaaacggc gtagcaggag agactttctg actgccctaa tcccgggcgt
    38041 tcccccacca gcaggatcac cactttcgcg ccgaggatct caccgatctg atcttcaatc
    38101 ttcacgcgac cataacgaac aaagaatggc gtaccgactt tcagcccggc ctgtttcagg
    38161 cccgccatca gcggtggcag gatctcttcg tagttcacgg tgatcgcatc agttgacagg
    38221 ccatcagaaa taacgacctg cacgtccgga ttagcaacac actgtgcttt cagggcttca
    38281 acagcttctg cacacaggcg gcggcccata tccggacgag tcaggtagag atttttgtcg
    38341 ctaatttcag agcggacttc cagcaagccc tgcgctttca cccactcttc cggcacttct
    38401 ttcagtacgg tatctttcga acgagagtga tcggccagga aacgcagcaa cgcctgggta
    38461 cgcggacgcg gaccggcacg accggtacaa acgcgggcca cggtgctgcg acgcagttct
    38521 gttaatacgt ctgcgcgatg cgggttttca acaccaatcc acgcttttgc ttccgcagag
    38581 cctaagtcca gcgcgcagct ttccgaggtc accggtgccg cgcaggtggt ggtggcgcac
    38641 tttgcttctg acggcgcggg ggccgcttgt cccattgacg ccatcacgct gcgtacaatt
    38701 tcttcaatct gtttttgatc catgatgtgt tatctccgcg tcatcagaag aacagtgacg
    38761 gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat gcccatgctt tccagccagc
    38821 gttcaaactc cggtgacggg cgcaggttga gtaactgacg cacagtggca gtgtcgtgga
    38881 atgcggtggt ctgatagttg agcatgatgt catcgcccag cggcatcccc atgatgtagt
    38941 tgcagcctgc ggtggcgagc aggatcatga ggttttcgtt gaggttctgg tcagcgtcag
    39001 cgtggttggt gtagcagcag tcacagccca tagagatgcc gctcagctta cccataaagt
    39061 gatcttctaa gcccgcacgg ataatctggc ggtcgttgta gagatactcc ggcccaataa
    39121 agccgaccac ggtgttgacg ataaacggat cgtaatgacg cgccagccca tagttacgcg
    39181 cttccatcgt cacctggtct gcgccgaagt tcgcgccagc ggatagcgcc gagccttgcc
    39241 cggtttcgaa gtagagacag ttttccccgg cgatacggtt gaattccgcg cccactgcgc
    39301 gtgcttcatc gagcatcgcc agctccacgc caaattcttt cagccctttt tcgctgccgc
    39361 aaatactctg gaaaatcagc ccgcccggtg cgccgcgacg aatcgcttcg atctgggtgg
    39421 tgacgtgcgc cagcacgcag ccctgggtcg gaatgttgaa tttgtcgatc acgccgtaga
    39481 tggtgtccag cacgcggctt aagttttcca cgtcgtccgt caccgggttg acgccgatca
    39541 ccgcatcgcc caccccgaag gaaagccctt cgtagatttg cgcagcgata ctttgcacgt
    39601 cgtcacgggt gtcgtttggc tgcaagcggg cgctgaaggt gcccggaata ccgatggtgg
    39661 tattggcctt tttgatcacc ggcattttct tcgcgccgta gatcaggtcc gcgttggagc
    39721 aaatcttcgc taccgccgcg accacttccg aggtcagccc tttgcgggta aaggcaatgt
    39781 cgtccacgct ggtttcatcg ctcagcacat actcacgcag ttcgctgatg ctccagtttt
    39841 taatctggtt gtaggccgtt tcgttaacgt cgtcctgaat cagccgcgtc acgcagtcat
    39901 cttcataggc aatcaccgga ttattacgga tgtccgctac cgtcatttcc gacaacacct
    39961 gctttgccgc cacgcgctcc tgtgagcttg ccgctgcaac gcccgccagc acatcccccg
    40021 aacgcagttc gttggcttta gccagcacct cttttacatc cttaaactga tatacattgc
    40081 cgaacaatgt ggtctttagt ttcataagtc gttccctcag gaaggaaatg cgagtgattt
    40141 caccgtcacc ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga tatagtcccc
    40201 cgcgcggaca atcacttcgt caatgactgc caacgggagt tgttgtagct gtgggcgcaa
    40261 caacatgccc agagctttac caaagtcctg cccggccacc accagcaggg gatgcggatt
    40321 cggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc gtcagtaccg cggcgtaacg
    40381 cacaggcagc gaggcgggaa gcgccagcac gtacgcgtca gaattggggc aaagatccag
    40441 ctgaatcagc gcctgttgcc aggcactcac cagatccgtt tcatcaattg ggatcgccac
    40501 cggcaaattg cgcagcggca gttgtacgcc ctccagccag attgtgctac cagagagcga
    40561 aagggtatgt gcacccgcgc caatcaccgt ggcgcgtacg gtttgcgccg gaaactgcac
    40621 gttcatctca cgcaggcgcg gatggtcatg cagcgccgtt gccagaagcg ggccgatatc
    40681 ggcaaaacag aacgggtcgg cgggctggtg gcgataacat tcgcccacgc cgccagaaag
    40741 cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc
    40801 gagcggcgag agcgttccgt caatcacttc gacaatcagc tctgccatcc gccgggtaac
    40861 ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc
    40921 cacaatcatc tgccccggtt tatgagcgta aaccacgcgc ccctggctgt cggtttccag
    40981 caggcgaccg ccgacgttga ggcaggcggt gccgctgatt tttccggcat cgaacagggc
    41041 gtagttcgca gtgccaccgc cgatatcgat attcagtacc cgacacagcc gttgttcaga
    41101 aagggtttgc gccccggcac cgtgaccggc gatcacggat tcgagatgcg gcccggcgct
    41161 ggcgacgaca aaatcgccca gcgactggga gagcgtcatc accgccgggc gagcgttgcg
    41221 ggttttcgcg ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat
    41281 gcccgcagcc tgatattgct caaaaatcag cgatttcagt tccgcttctt ttaaaccacc
    41341 ctgtttatcg acaggggtaa agaacaccgg gctttgccag ctaatttcgc gtttgatgaa
    41401 ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc agctccaggt gggagaagat
    41461 cacctgggtg gtggtggtgc cgatatcgat accgacgctc agtagctggc gagtgttcac
    41521 gattgcgcct ccgcttcggt tttagccgtg gtcgcgtctt cttttggcac cagcatcatc
    41581 gccacgccaa ttgccgttac gccgccgatc aatttgccga caatcatcgg gaagatcatg
    41641 gcgttcatgt tggcagcggc gaagcctaag tggtcaccca gggcgaaagc agcggaaacg
    41701 gcgaaggcgc agttgatgac tttgccgcgg gtatccatct gcttcatcat gccaaacatt
    41761 gggatgttgt tggcaagcgt tgccaccatg ccagctgccg cgatattgtt catattcagc
    41821 actttaccga cgctcatcag cggtttttca aaccagcggg tcagcagcaa taccatcggg
    41881 tacgccccta acagaacaca ggagatagaa ccgataactt caatggcgcg catcacttca
    41941 ccgggtttat cgccaggggc cataaagata ggatccagac cggggatcag ttcccagcca
    42001 aggaggaatt tcactaccgc ggcagcaaga ccgagggtga tcaatgcaac gaggaatttg
    42061 gcgaagatct ggaagccgtt gatcattttt tccgggatga atttcagccc cagcgccacc
    42121 agcaccgcaa cgataagtac cgggatcatg ttcatcagga tcagcgcaaa ggtgaattcc
    42181 accggctgac cgttgatctg cacaccggag tacatagcaa ccagaccacc agcaatacag
    42241 ccaatcggaa tggtcacaat gcccgccagc acgccgagcg ccagataacg acggtcagaa
    42301 ggttcgataa tgccgagcgc caccggaatg gaaaacacaa tcgttggccc catcatcgac
    42361 ccgagaatta atccagagta tagccacgct gctacgtcgc cgcccgccag ctctttggcg
    42421 aggaagaagc cgcccatatc gcacgccagc agtgttccgg cgaacattga cgggtttgcg
    42481 ccgagcattt cgtaaaccgg gataatcacc ggcccgagta cgtgggccag taccggtgcc
    42541 agcgcggtca taccgaccat agccaggccc aaagcgccca ttgccataaa gccttcttcg
    42601 aactgaccgc cggatccttc gatactttta ccgaacttac cgaggaaacg agcagaaccg
    42661 ccgaactgcg acaggatcct gtctacggca gctatcagca taaagaacat catgatgtac
    42721 atgatgattt cgttaattcc catcgccttt actccctgtt agttgttatt tattggcgga
    42781 tgcggcgtaa acgccttatc cgccctacat gtgcaatcct gtaggccgga taagacgcag
    42841 caagcgtcgc atccagcaat tgcaccgcgc cactggcgga tgcggcgtaa acgccttatc
    42901 cgccctacat gtgcaatcct gtaggccgga taagagcagc aagcgttgca tccggcaatt
    42961 gcaccgcgcc actggcggat gcggcgtgaa cgccttatcc gccctacatg tgcaatcctg
    43021 taggccggat cagacgcggc aagcgtcgca tccggcaatt gcaccgcgcc actggcggat
    43081 gcggcgtgaa cgccttatcc gccctacatg tgcaatcctg taggccggat aagacgcggc
    43141 aagcgtcgca tccggcaatt gcaccgcgcc actggcggat gcggcgtgaa cgccttatcc
    43201 gccctacatg tgcactcctg taggccggat aagacgcagc aagcgtcgca tccggcattt
    43261 attgtgccgc tgcgtacagg ccgacaatct gctccaggct ggcggtacgc gggttgctgc
    43321 gcagacaaat atcttccagc gcggcctgcg cccatgcgcc gtaatgtccg gtggttgcgc
    43381 ccacgtctgc cagccgcttg ttgatcccga cttccgcaat cagctcactt accgcgttaa
    43441 tagcgtcacg atcgtcggat tttttagttc gcagcgcccg accaatctga ctaaagcgtt
    43501 cacgacaaac catccggtta aactccatca ccgttggcag tagcatggcg ttcgccagac
    43561 cgtgcggaat atgcagcgcc gcccccggct gatgcgccat cgcgtggcat aacccaagcc
    43621 ccgcactgga aaacgccatt cccgccatac atgaagccag caacatgctc tcgcgcgcgg
    43681 caaggtcgtg accgtagccc accgctttcg gcagcgattt gccaatcatc gcaatagcgc
    43741 caatcgccag gctgtcggta aacggtgtgg cgttcagcgc gctgtacgct tcaatggcat
    43801 gagttaacgc gtcgataccg gtcatcgccg tgacatgcga cggcacacct tcggtcaatg
    43861 cggcgtcaag aatcgccaca tccggcatca gcgaggcatg gactaacacc tgcttgcgct
    43921 cgctcaccgc gtcgataatc accgttacgt tggtggtttc agagccggtt ccggcggtcg
    43981 ttggaatggc aatcaacggc aggcgcggtt gcagaacgct ggtttctgac atctctgcca
    44041 gcgtgctatc cgggttcgtc accagcaacg ccacggcttt tgctgcatcc agcaccgagc
    44101 cgccgccaaa cgcaatcacc ccctcacagc ctgattcacg caactgcgcc acggctgcac
    44161 acacgtcggt gatgcatggt tcgcccaccg gacatggcca gagcgtcatg gcgatacctt
    44221 taacggccag gctacgcgtc agcccggcgg tcatccctgc ctgatgcaaa aagctgtctg
    44281 ccatcacgaa cagatgtttc agcccacgcg tttgcgcttg ctgcccacaa ctgctcaccg
    44341 cgcccggacc gcaaagcgtc accggtggaa cgctaaatgt ttttacccgt tgcagattca
    44401 gggtatcgaa cgcctgaaag agcgcggcct gcaattcatt ttgcatagat cccctccgct
    44461 ttctctcgcc cgctactggc gatcgccagc ggggtcataa acaaactgtg ctgcggtaga
    44521 tgcacctgta acgccgggaa ttgtttgcaa aacagttccg ccacgcccgg ttgcatacag
    44581 gagccgcccg ccagccataa atcagtaatg ccctgccctt caatatgccg ggcgacaatg
    44641 tccgccattt tttcgtacac cggtttcacc gcaggccaaa tctcgtcacc gtgaccgcgt
    44701 ttgtactgct ctgcctcttc cagcgaaata cggcgatttc cggcgagagt cagagagatg
    44761 tgatgtccgc cggtggcttc atccgccgag tacgtcactt tgcccttttt cacgatggca
    44821 atgccggtag tgccgccgcc gatatccact acaccggcgt tgtccagttg cagcagatcc
    44881 gcaactgcgg ttggctcatc cagcacatgg ctcacttcca gtccggcaga ttccagcacg
    44941 ttgatggaaa tacgcgggtc cgtacctgga ggaaaagagg tcgccgcatg gctaaaacgg
    45001 cgaccgaatt gctgctcgag ggtgtcgaga tggcgacgaa caatggtgac cgcaccgaag
    45061 aaatcccaga cgatgccgtc gcggaccaca tcagcccagt cgaggcatac cgccaccggc
    45121 tgaccgtcgc ggtcgacaac catcgacacc acatcgcagg tgcccagatc cacccccagc
    45181 cacagcggag attccgtcgc ggcaggcgtc tggttacaca gcgttgccgc cgtttgcaga
    45241 cgtggggtca gccattgttc gtcgtgcgcc atctgttact ccttaaacaa tgcgaaacgc
    45301 atcgactaat acacagcgac gcagacggac aaacgtccgc gcgctggtta ccccttcacc
    45361 ggttggcgtg gtgatggtca tggtggtcca gccttccccg cccagcccca gcccggcaat
    45421 gcacggtccg ttcttaacga aaatgctggt atcaatggca ttcgccatct ggttcatgtt
    45481 ctcgatattg cgcgagtgca ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc
    45541 cagcgcaatg gcatccgcca cgttggcgac gcgcacgacg ggcagcaccg gcatcatcag
    45601 ttcggtcacg gcaaacggat gttctgcggt ggtttctaca aacagcagtc gcgtttgttc
    45661 cggaactgta aggccgattg ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg
    45721 gctgacggtg ccttttccgt gctcgtcgat atttttcagc aacaccggtt gtagctgctg
    45781 cgcctgctct gcggtcagtt tcaccgcgtg ctggccttcc atcagacgca tcagttcatc
    45841 ggctacgcta tccacaacaa tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa
    45901 agacgcgcct ttgacgatgg actgagctgc acgggcgagg tcagcggttt catccaccac
    45961 taccggcggg ttgccagcac ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc
    46021 ttctactacc gcttcgccgc cggtcaccac cagcaggccg atgcccggaa acttgaacaa
    46081 acgttgagcg gtttcgatat ccggatttgc cacagtaacc agtaagtttt ccggcccgcc
    46141 tgcggcaaca atcgcctggt tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc
    46201 cggatgcggg gcaaaaatga cgctgttgcc cgcggcaatc aggctgatgg cgttgttaat
    46261 tacggttgcc gccgggttag tggaaggcgt caccgaagcc accacgcccc agggtgcgtt
    46321 ttcaatcagg gtcaggccgt tgtcaccggt cagcacctgt ggtgagaggc actcaacgcc
    46381 tggtgtgccg cgcgcctgag cgacgttttt ggcaaattta tcttcgacgc ggcccatgcc
    46441 ggtttcactg acggcaagtt ccgctaaatc tctggcgtgt ttttcgcctg cttcacgaat
    46501 ggcagcaatg gctaactggc gcattgccac gctttttaac ccttgctggg cgactttggc
    46561 tgccgcaacg gcgtcatcca gggacgcgaa aacgcccatc tcatgaacgg cggcgggcgg
    46621 cgtgtcactg ctttgcattt tcagcagtac cgctttcacc acctgttcaa tatcctgttg
    46681 attcatgatg ttctgcctta tttgtggaaa attacctgac cgccagacac cacctcatcg
    46741 acgatgccaa tcacgcacag atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg
    46801 gaactgccac tcaccagcaa cacccactcc ccggttcccg cgccaatatt gtcgatggcg
    46861 acggcgcatt gcccgtcagg attaccttgt ggatcaatca tttccaccat cagcaatttg
    46921 tcatgcgcca gtccgtgatg gcgtacggta caaacaattt gtccagtgac gactgccagt
    46981 ttcatacccg cctccgtggc gtatttcagg taaaagctgc ccctaccctc cgcggaaggt
    47041 aaactgaaaa aggagagagc gtgacgcccg aatcgacgtc acacagggtg attacaggtt
    47101 gctgctatcg cctttcaggc cgatcgggaa gacttcttcc aggtcgccgt gcggacgcgg
    47161 gataacgtgt acagatacca gctcgccgat acgctgtgcc gctgcagcac ctgcgtcggt
    47221 cgcggcttta caggctgcaa catcaccacg aaccatggca gtacacaggc cgccgccaat
    47281 ctgcttaaca ccaaccagct tgacgcgcgc agctttcacc atcgcgtcag aagcctcaat
    47341 cagtgcaacc aggccccggg tttcgatcat tcctaatgct tccattgtgc tttcctcttt
    47401 atcagggtcc agaacgggac cgttcattca accagtgttt gtaaactgct ttcgcggttc
    47461 acttctgtct gacgcggcac agctgccacc agcgccagct cgataatttc ctgcacgcta
    47521 caaccacgag agagatcgtg catcggcgcg gcaagtcctt gtatcaatgg cccgacggca
    47581 cgatatccgc cgagtcgttg tgcgattttg tagccaatat ttccggcttc cagcgacgga
    47641 aaaaccatca cattggcctt gccttgtagc gggctggcag gcgctttttg cgccgccact
    47701 tccggcacga aggcggcgtc aaactgcaac tcgccatcga ctaccaactg cggcgcgcgc
    47761 tcacggacga tttctgtcgc ctgctggacg ttggcaacgc aggggtgacg ggcgctgccg
    47821 ttactggaaa acgacaacat cgccacgcgc ggctcttctc cggtgatggc gcgccaggtt
    47881 tcggcgctgg caagcgcgat atccgccagt tgtgccgccg tcggctgtgg caccacgctg
    47941 caatcggcaa aacccaacgc cggaccgctg tactgtggca gcatcaggaa aatggaggag
    48001 agcgttttac agcctggctg taagccaata atgcgtaatc cggcacgcag cacattcgcc
    48061 gtggaagaga gattgcccgc gatacagaca tccgctgtcc cggcgctgac cattgcggca
    48121 gcgaacatca gcggatcggt gagtttttcc aacgcatccg gcggcgtttt ttcgcccgca
    48181 cgggccagcc agcgatgggc aaattcttcc cgcattgcga ggttgccatg cggatctatc
    48241 acctgtagcc cgtccattgc cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga
    48301 ttggcgacca gaatgggcgt tgccagacct tgttgatgta aatattgcgc ggctttcagc
    48361 acacgttgat ctaacgcatc cggaaaaacc actctggcgg gcgctcgcaa cgccagttca
    48421 cgacaacgtt caataatcat tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta
    48481 gaggctgctt cgttacgctc aggatcatca tcacgtagac cgtgctcgaa aggcggttca
    48541 gcgcctgcaa gatgtccggg cgtaatactt caaaactgcg ggttataaac acctgcgccg
    48601 ctacggtttc ggtttcccgc actttggtgc gtagcagatt gagcaacgca gcatcgcgcc
    48661 cgtggctggc ttccggcacc agatgatcgt gaccaagata gcgcagcggc tggtgagaaa
    48721 gccgatgcag atcttcgtca gaaagcccga caatcgcctg gcaggccagc ggttccccca
    48781 gcgcatcggc acgcataatg ttgccaagac gcgaacggat atccgccagc cacggctgcc
    48841 acggttccgc cagttcaatt tgcagccaca ctgccagcgc aatggtgctg tcgagaacag
    48901 cgcgaaaacc gaggcgcgga tcgcttttgg cgaccatttt ttccgccgac aggtgagtca
    48961 gagtgtccgg cttttttgcc accggctggc ggcacagttc gcagcacgcc tgcggatgtt
    49021 catcgctact ggtcagcccg tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca
    49081 ggcggccctg ctcgtcaata aacttgatgc gcagatggcg gctttccagt agctcccggg
    49141 cagagggcgt caggcgactg tccgcaggga gatggatctc tgcgccttcg ctgagcgtat
    49201 ggttcgctct tagccatgct tcggtgatga aatctttcat agggattgcc agttagccgg
    49261 ccaggcgaca tacaggaatt tcacgctgga tgtcgtacca aattcgatgc tggagccttt
    49321 cgggataaac atcacgtcgc ctgctttggc gatcatggtt tcgccttcgt ggcgaacgtg
    49381 cagttcccct tccagtacca tgtcgatttc gtcatagttc agggtccacg ggaagaaggc
    49441 gttctcccac tgcataaaac cagcggccat gctgctgccg tcgtctccgg tgaccagatc
    49501 cgttaatccg acgcagtgtg gctgtgcgcc gtcaaaacga ccaaacttga cgctgctgcc
    49561 atcaatcact ttgatgccgc ctttgccggt aaccgatttg aaactcggct gcattgcacc
    49621 ctgctccagt gactgttttt ccttcatcac tttttccatc agctgcgcga ccaggctttc
    49681 ggtaaactgg ccttccggta gctgggcgat gatggtttcg cggatacgct ggctttcggt
    49741 tttatcggct ggcacgcagg cgggaacgga cgccgttacc ggggtcgatt catcacattc
    49801 agtgatggta aaacccagta gttctgccac ttcacgggct tccggggtaa taatgctggc
    49861 gcgcagaacc acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc
    49921 tgtgatgagt tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca
    49981 gttgctgcac gctttgcggg tcgtggctgt ttagctcgaa aatcggctct tcgaagcccg
    50041 tctcaagcaa taactttcgc gttgcggtga cgtcagcatc tggcatgtcc gttttactga
    50101 tgacggcgat ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc aaccgacttt
    50161 ccgggtcgtt cgcgccgtgg acatagatca gcatgtccac atcctgtagc gtggtaatta
    50221 aggcgtgata ccagcgggga tggctaaaat attcacccgg cgtatcaata tcacccttat
    50281 cattaaattc tacggcctgc gtttttctgg cgagggtata atctccctgc aacgcattaa
    50341 ataacgttgt ttttcctgcc ccgacggtac ccacaaacgc aattcgtttc atgaatgcct
    50401 cgtattaaca tttggtcatt tcacaaagcg tataatttaa taaacgccct aaaccgctga
    50461 cggtttgtga taacgcttct tctactgcac ccaccgagcc ataaatcacc aacgcgccgc
    50521 taaaacggtc gagaaaaccg atatgcacat cggcggcttt tagtgctaaa tcaccggcga
    50581 tcatcgccgt ttcgccggga gttagcgtca taatgccgat tgcacccgca tcaggaacgc
    50641 cgattttctt cgccagttcc tcgccgggat gcgcgatgag atgcgccagt gtgacctgtt
    50701 taccgggcac aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa
    50761 ctttcgtgta tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcgcgttt
    50821 aaaaggtgag gtagatcact aagaatgga
//
LOCUS       DAYPHN010000012        49665 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000012 DAYPHN010000000
VERSION     DAYPHN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.012"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..590)
                     /locus_tag="ACG0BW_000745"
     CDS             complement(<1..590)
                     /locus_tag="ACG0BW_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HFK7257744.1"
                     /translation="MDTFHNILLEHIDSLLKERLDLSGDADIEKEINNLIPKIAIVVK
                     KSLIGSANAMLREHRSLCDEFVERNISRWAEAFDLLETLIVICTESGEEFNRSYRPQA
                     ASEEDVVFDLVVRHHARACHIANEILCLLKNGFADAAQARWRALHEVAATAMFIAKHG
                     KECAERFYYHEVVDSYTGMLEHKKYEHRLEAKGPTI"
     gene            complement(818..1243)
                     /locus_tag="ACG0BW_000746"
                     /pseudo
     CDS             complement(818..1243)
                     /locus_tag="ACG0BW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
     gene            complement(1284..2354)
                     /locus_tag="ACG0BW_000747"
     CDS             complement(1284..2354)
                     /locus_tag="ACG0BW_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFK7257745.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTPRPEQSHQL
                     QQPEADSAIQREADRVVPENTGLSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            complement(2426..2851)
                     /locus_tag="ACG0BW_000748"
     CDS             complement(2426..2851)
                     /locus_tag="ACG0BW_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFK7257746.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELYD
                     DSHPEALARNTQKIYRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(2835..3116)
                     /locus_tag="ACG0BW_000749"
     CDS             complement(2835..3116)
                     /locus_tag="ACG0BW_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFK7257747.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            3217..3636
                     /locus_tag="ACG0BW_000750"
     CDS             3217..3636
                     /locus_tag="ACG0BW_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257748.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            3902..4057
                     /gene="ydfA"
                     /locus_tag="ACG0BW_000751"
     CDS             3902..4057
                     /gene="ydfA"
                     /locus_tag="ACG0BW_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFK7257749.1"
                     /translation="MDTIDLGNNEALVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4059..4160
                     /gene="ydfB"
                     /locus_tag="ACG0BW_000752"
                     /pseudo
     CDS             4059..4160
                     /gene="ydfB"
                     /locus_tag="ACG0BW_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            5122..5310
                     /gene="dicB"
                     /locus_tag="ACG0BW_000753"
     CDS             5122..5310
                     /gene="dicB"
                     /locus_tag="ACG0BW_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFK7257750.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            5307..5498
                     /gene="ydfD"
                     /locus_tag="ACG0BW_000754"
     CDS             5307..5498
                     /gene="ydfD"
                     /locus_tag="ACG0BW_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFK7257751.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            5591..8062
                     /locus_tag="ACG0BW_000755"
     CDS             5591..8062
                     /locus_tag="ACG0BW_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFK7257752.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDNLAPEVQNAVMVKFHTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPSAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARA
                     RANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGHSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGETSDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADS"
     gene            8135..8386
                     /locus_tag="ACG0BW_000756"
     CDS             8135..8386
                     /locus_tag="ACG0BW_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFK7257753.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8421..9701
                     /locus_tag="ACG0BW_000757"
     CDS             8421..9701
                     /locus_tag="ACG0BW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7257754.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLVDNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(9889..10908)
                     /gene="rspB"
                     /locus_tag="ACG0BW_000758"
     CDS             complement(9889..10908)
                     /gene="rspB"
                     /locus_tag="ACG0BW_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFK7257755.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(10920..12134)
                     /gene="rspA"
                     /locus_tag="ACG0BW_000759"
     CDS             complement(10920..12134)
                     /gene="rspA"
                     /locus_tag="ACG0BW_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFK7257756.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(12340..12666)
                     /gene="ynfA"
                     /locus_tag="ACG0BW_000760"
     CDS             complement(12340..12666)
                     /gene="ynfA"
                     /locus_tag="ACG0BW_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFK7257757.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRA"
     gene            12801..13142
                     /gene="ynfB"
                     /locus_tag="ACG0BW_000761"
     CDS             12801..13142
                     /gene="ynfB"
                     /locus_tag="ACG0BW_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFK7257758.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            13177..13737
                     /gene="speG"
                     /locus_tag="ACG0BW_000762"
     CDS             13177..13737
                     /gene="speG"
                     /locus_tag="ACG0BW_000762"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFK7257759.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13740..14450)
                     /gene="ynfC"
                     /locus_tag="ACG0BW_000763"
     CDS             complement(13740..14450)
                     /gene="ynfC"
                     /locus_tag="ACG0BW_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFK7257760.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     HDKTLSVSATPSTDPIKKLDYTAVTILNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAIERVYTIKNTIDYY"
     gene            14558..14863
                     /gene="ynfD"
                     /locus_tag="ACG0BW_000764"
     CDS             14558..14863
                     /gene="ynfD"
                     /locus_tag="ACG0BW_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFK7257761.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            15062..17488
                     /gene="ynfE"
                     /locus_tag="ACG0BW_000765"
     CDS             15062..17488
                     /gene="ynfE"
                     /locus_tag="ACG0BW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFK7257762.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPEVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            17549..19972
                     /gene="ynfF"
                     /locus_tag="ACG0BW_000766"
     CDS             17549..19972
                     /gene="ynfF"
                     /locus_tag="ACG0BW_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFK7257763.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEVPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIHLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            19983..20600
                     /gene="ynfG"
                     /locus_tag="ACG0BW_000767"
     CDS             19983..20600
                     /gene="ynfG"
                     /locus_tag="ACG0BW_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFK7257764.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            20602..21456
                     /gene="ynfH"
                     /locus_tag="ACG0BW_000768"
     CDS             20602..21456
                     /gene="ynfH"
                     /locus_tag="ACG0BW_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFK7257765.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARITFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            21499..22113
                     /gene="dmsD"
                     /locus_tag="ACG0BW_000769"
     CDS             21499..22113
                     /gene="dmsD"
                     /locus_tag="ACG0BW_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFK7257766.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQAQSEETRAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            22271..23563
                     /gene="clcB"
                     /locus_tag="ACG0BW_000770"
     CDS             22271..23563
                     /gene="clcB"
                     /locus_tag="ACG0BW_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFK7257767.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHREFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(23516..24211)
                     /gene="bioD"
                     /locus_tag="ACG0BW_000771"
     CDS             complement(23516..24211)
                     /gene="bioD"
                     /locus_tag="ACG0BW_000771"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFK7257768.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(24336..25556)
                     /gene="mlc"
                     /locus_tag="ACG0BW_000772"
                     /gene_synonym="dgsA"
     CDS             complement(24336..25556)
                     /gene="mlc"
                     /locus_tag="ACG0BW_000772"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFK7257769.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEEAQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(25691..26584)
                     /gene="ynfL"
                     /locus_tag="ACG0BW_000773"
     CDS             complement(25691..26584)
                     /gene="ynfL"
                     /locus_tag="ACG0BW_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7257770.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            26691..27944
                     /gene="ynfM"
                     /locus_tag="ACG0BW_000774"
     CDS             26691..27944
                     /gene="ynfM"
                     /locus_tag="ACG0BW_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7257771.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     FFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(27993..28112)
                     /locus_tag="ACG0BW_000775"
     CDS             complement(27993..28112)
                     /locus_tag="ACG0BW_000775"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFK7257772.1"
                     /translation="MTHSKMNPFIWVLIVLMTLDALRELAGASSILGWLLTLV"
     gene            28368..28676
                     /gene="asr"
                     /locus_tag="ACG0BW_000776"
     CDS             28368..28676
                     /gene="asr"
                     /locus_tag="ACG0BW_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFK7257773.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKTEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            28769..28852
                     /locus_tag="ACG0BW_000777"
     CDS             28769..28852
                     /locus_tag="ACG0BW_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257774.1"
                     /translation="MVGRYRFEFILIILILCALIATRFYLS"
     gene            28952..29773
                     /gene="ydgD"
                     /locus_tag="ACG0BW_000778"
     CDS             28952..29773
                     /gene="ydgD"
                     /locus_tag="ACG0BW_000778"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFK7257775.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDRLDQLSQK"
     gene            complement(29812..30141)
                     /gene="mdtI"
                     /locus_tag="ACG0BW_000779"
     CDS             complement(29812..30141)
                     /gene="mdtI"
                     /locus_tag="ACG0BW_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFK7257776.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(30128..30493)
                     /gene="mdtJ"
                     /locus_tag="ACG0BW_000780"
     CDS             complement(30128..30493)
                     /gene="mdtJ"
                     /locus_tag="ACG0BW_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFK7257777.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            30905..31939
                     /gene="tqsA"
                     /locus_tag="ACG0BW_000781"
     CDS             30905..31939
                     /gene="tqsA"
                     /locus_tag="ACG0BW_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFK7257778.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALFVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(31964..33352)
                     /gene="pntB"
                     /locus_tag="ACG0BW_000782"
     CDS             complement(31964..33352)
                     /gene="pntB"
                     /locus_tag="ACG0BW_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFK7257779.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(33363..34895)
                     /gene="pntA"
                     /locus_tag="ACG0BW_000783"
     CDS             complement(33363..34895)
                     /gene="pntA"
                     /locus_tag="ACG0BW_000783"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFK7257780.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNLGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            35419..36363
                     /gene="ydgH"
                     /locus_tag="ACG0BW_000784"
     CDS             35419..36363
                     /gene="ydgH"
                     /locus_tag="ACG0BW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFK7257781.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSYYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            36549..37931
                     /gene="ydgI"
                     /locus_tag="ACG0BW_000785"
     CDS             36549..37931
                     /gene="ydgI"
                     /locus_tag="ACG0BW_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFK7257782.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            37968..38690
                     /gene="folM"
                     /locus_tag="ACG0BW_000786"
     CDS             37968..38690
                     /gene="folM"
                     /locus_tag="ACG0BW_000786"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFK7257783.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLSDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHASSDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(38687..39022)
                     /gene="ydgC"
                     /locus_tag="ACG0BW_000787"
     CDS             complement(38687..39022)
                     /gene="ydgC"
                     /locus_tag="ACG0BW_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFK7257784.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            39151..39870
                     /gene="rstA"
                     /locus_tag="ACG0BW_000788"
     CDS             39151..39870
                     /gene="rstA"
                     /locus_tag="ACG0BW_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFK7257785.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            39874..41175
                     /gene="rstB"
                     /locus_tag="ACG0BW_000789"
     CDS             39874..41175
                     /gene="rstB"
                     /locus_tag="ACG0BW_000789"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFK7257786.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            41251..42180
                     /gene="tus"
                     /locus_tag="ACG0BW_000790"
     CDS             41251..42180
                     /gene="tus"
                     /locus_tag="ACG0BW_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFK7257787.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(42177..43580)
                     /gene="fumC"
                     /locus_tag="ACG0BW_000791"
     CDS             complement(42177..43580)
                     /gene="fumC"
                     /locus_tag="ACG0BW_000791"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFK7257788.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELAMGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(43723..45369)
                     /gene="fumB"
                     /locus_tag="ACG0BW_000792"
     CDS             complement(43723..45369)
                     /gene="fumB"
                     /locus_tag="ACG0BW_000792"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFK7257789.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            45568..46743
                     /gene="manA"
                     /locus_tag="ACG0BW_000793"
     CDS             45568..46743
                     /gene="manA"
                     /locus_tag="ACG0BW_000793"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFK7257790.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            46844..48352
                     /gene="ydgA"
                     /locus_tag="ACG0BW_000794"
     CDS             46844..48352
                     /gene="ydgA"
                     /locus_tag="ACG0BW_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFK7257791.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLSSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPALPQQ"
     gene            complement(48397..49662)
                     /gene="uidC"
                     /locus_tag="ACG0BW_000795"
     CDS             complement(48397..49662)
                     /gene="uidC"
                     /locus_tag="ACG0BW_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFK7257792.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT        12788 a        12594 c        11845 g        12438 t
ORIGIN      
        1 tcaatagttg gaccttttgc ttctaaccgg tgctcgtact ttttgtgttc caacattcca
       61 gtatatgaat caaccacttc atggtagtaa aagcgctcag cacactcttt tccatgcttt
      121 gctataaaca tagctgtggc cgctacctca tgaagtgctc gccatctcgc ttgggcagca
      181 tctgcaaatc cattctttaa gagacacaaa atttcgttgg cgatgtggca agctcttgcg
      241 tgatgccgaa cgactaaatc aaaaaccaca tcctcttcag aggcagcttg tggtctataa
      301 gaacgattga actcttcacc ggactccgtg catatcacta taagtgtttc gagcaaatca
      361 aaagcctctg cccatcttga aatatttctc tcaacaaatt catcacaaag agaacgatgt
      421 tctctaagca ttgcattggc cgagccaata agagattttt taacgacaat ggctattttg
      481 ggaattaggt tattaatttc tttttcgata tcagcgtcac cagataaatc aagtctctct
      541 tttaaaaggc tgtctatgtg ctcaagcaaa atattatgaa aagtatccat gtcatatctc
      601 aataagtttt aaatccatgt ggcacaccca taatataaaa ataaacagca tgccagtcta
      661 agtctggcta tttgtatatc atatcacctg aaacattgac agatgtaacc caccaaaata
      721 ttatcaatga ccactcttgg cacaaagcga acaaccacgc tagctctacc ttgtgccatg
      781 aaaatgtcaa tgcatatctg aactaatgcc ctttaatcta gtaacgtcta aaatacctaa
      841 catttccctg ataaaatgcc agaatgcgct gcataacttc gctcttccgg cactcgcgac
      901 agattatgtt tagacaacta tcgtagcgac gtatttctcc gtcaggtaat gaccagataa
      961 ggtccggatc aaccgcagat ggtttcttca gctttgccct tgagagcttt atacgggcat
     1021 tttgtcagtc cttacgcgcc tgttcagacg ggaataaccc gtaaccagag ttgtatacat
     1081 cgccactggc aaccagctct ctggccagaa cactcatcag atatcttgtt gccccagttt
     1141 tagtttccag ttgtcgtaac gtctcgcgcc cactctggcg tacgagttca acaacctgcc
     1201 ctttaatttt ttcccgctct tcttgtgtaa aaacttttgc cacaagtcct cctgaaaatt
     1261 acctcatgac cagaaattaa cacttacccc ctgaagcccg gcggaatttc gttatccggt
     1321 tcagaaatat gattcacaca acgctggttg ttcgtgccgc ttaccgggag caaccagggg
     1381 ttctcaaaat tccggtccgg tccaaaaaac gtcgtcgctc gctgaacaaa ttccgttccc
     1441 gttttcccgg tagccgccaa gtatcttgcg taacgcctca cgccatccag catggcctct
     1501 ggtggcaccc cctcgcgtaa tctggccttc caggcactga aagcggattt cttcgggttt
     1561 gctccggcac gcaacgggta ctcccgccag acctgttcga atacatccgg ataatccact
     1621 cgtcccacag acagcccggt gttttccggg actacccgat cggcttcccg ctgaatggcg
     1681 gaatcggctt caggctgctg cagttggtgt gattgctccg gccttggggt catcacctgc
     1741 tgcacagcgc ccgaatcggc tttcagcgca tacgctgaat cggcttccgg tgtcgtgcct
     1801 gctggctgac caggattgac ggtctgaaca tcccctgcct ggttcgtggc gttttttacg
     1861 ccatggacca tagtgttttg atcttcttga tctgtatctt tatctgtatc tttatctgtc
     1921 gtgactcgtc gtgacatgtg cgtgacattt cgtgacgcgc cgtgacaatc gccattttgt
     1981 tcccgctttc tttccctctc tcgctgcgcc ctcttgcgct ctgccggaga ttttgcggtt
     2041 tgcgaaatat tgccgttgtc ctctttaagc acctggcgtt tttcccatcc agtgattaaa
     2101 tcaccatcaa gtacccgccc ctgcatcgtc tgcaaaattg aatcaattac ctcttctgtc
     2161 acgtcgagcg cacttgctaa atcttctgtc gtgacatcaa tgtgacctcg cgtgacattt
     2221 cgtgacgcgc tcaccaggag gtggatatac actgccatca ctgttgcaat tggctgccct
     2281 gacaccctgg caattgttcg ccacttaggg tcatttggca tgtcatgcca taatctgagc
     2341 caagcgttag ccatactcac ctcttctgat accgaatctt tttactcacg agttgccgga
     2401 agcgattcga tatggctatt gtcagtcaat gtactgccac agcatttcct gccgggccac
     2461 cacggttcat ctgattgaaa ccggcgattg ccactgcgac aaaatcatca gcgtctctca
     2521 ccagtcgctc ccgcgtctcc accagctccc gaaaataagc tgaactgtgg ctgcgcattc
     2581 tggccaccag caaaggtggc attgcctttt cgatcgctgg taacaacgcc tgaatttttt
     2641 caactgcatc aggggtgtct ttctctaccc agcggtaaat tttctgggta ttgcgagcca
     2701 gggcttccgg atggctgtcg tcatacagtt ctgggaacgt catacccagt tcaaaataag
     2761 ccctggttat ttcagctgcc ggaacttttt caccgtccgg atgcgcccag gcattcatcg
     2821 ccatgcggat gtgttcatgc ttgattttca tgaatcaagc tcctagaaag tggttgtgtt
     2881 aacgttttgg tatcttccag ctcgggccaa atattcatcc aatcaaaagg ccttagttgc
     2941 tgacgtgtaa cttcaccatt actggctcgc tcaataagga cacataacga tgcccctaac
     3001 acttgacctt tactcaatgc ctttcttaga taaccgatgc tggtaccaca ctcgcatgca
     3061 aacatacgct gttcatctga cgaaagagaa ttgagaaata ttcttaattc ttccatagct
     3121 actccttagt aaacacagca aagaatacct acaggtaaac aaaagtcaat acccacaggt
     3181 tgtttacctt gcagtaatcg catctattat ttacctatgg acaaatatga atttagacga
     3241 cagcaactca tcaaaattcg tgatgagaaa tgcgatggta aagcggttaa cgtggccaga
     3301 aagatcgggc gcgagccttc ttatgtatca agaatgttgt acccagaggg gaaaaaggga
     3361 aaaaaacgga tcgctgatga tatggtggag attatcgaag agtcctttgg gttaccccgg
     3421 ggatggatgg atggtatcgt ttcatcatca acgaacacag cctccagtta tgaaacaagg
     3481 gttctaacgc cacgacaacg tattttttta gatctcttag acgaactgcc agaaagtgaa
     3541 gcggataact tattaaaaac tcttgaagag aaaaaacagt attacaatat gatctacgaa
     3601 gaaatccgta aaaagaaagc acaaaacgca tcatagctca ccaaacaact agtcaccagt
     3661 taagacaccg caaaaattta cccatgggta tttacttttt aaatacctat gggtatcctt
     3721 cttttcatac caactcaccc cgctccacag aatgcagggc aatacttcga gttaccaggc
     3781 agtggtcagg ggttaagtag ccagcccgag gcgtaagaac atgacggcag ggttcaactt
     3841 taataactat gcagcaggtt tttgttccgc taccccggcg ttaaggggaa atgaggtcag
     3901 catggatact atcgatcttg gtaacaacga agctctggtg tacggcgtgt ttccaaacca
     3961 ggacggcacg ttcaccgcaa tgacgtatac caaaagcaaa acgtttaaaa ccgaaaatgg
     4021 tgcccgtcgc tggctggaaa gaaactcagg tgagtgatat ggatttcgac acaatcatgg
     4081 aaaaggctta cgaagaatac ttcgaaggcc ttgccgaagg cgaagaagct ctcagcttca
     4141 gtgagtttaa acaggcactc agaataagaa tgtgctctca caatgacgcg gagcacaaat
     4201 atgagaagca aaatcagacc gcagaaaatt ttgttctgga acccggagaa acgcttttca
     4261 aaattcccgt tacgtgcccc atttgcggtt ttacatcaga agaacttgac gactcctgta
     4321 acaatcagga aacaaccaag tatgtcgaag atgataccga gtgcgcacga agaacgatta
     4381 tatccacgag tccaaactcc aggaccaata aatctcactt tgagagggtg attaatccac
     4441 tcccccaaac caataaaaaa gatgccggag gacaaaaaac ccaatggaac aacgggatat
     4501 ctgtcaaaaa aagacgttcc attaaagaca aacaacgcag cgccaacaac tgtaagcgct
     4561 ttataccagt aatcaatttt catgttctta agcggattta ttggtggttg cgacattgcg
     4621 taatgaatcc ttaaaactgt ggtgatttta aggataccac ctcgcctgac gtggttaaaa
     4681 gcaggcacac aacacgaaag cgcacggcga agttcgtctc actgtacggt gtcgttaaat
     4741 ttaattcgac cgtgcgcttc cggttgtggc aacccgcgaa atggcgcggc ggtaagtatg
     4801 gcggggttat tccttccccc gttgaggaca ccgggttgtc aggttgacca tacgcttaag
     4861 tgacaacccc gctgcaacgc cctctgttat caattttctg gtgacgtttg gcggtatcag
     4921 ttttactccg tgactgctct gccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
     4981 ggctgagcgc acgcggaaca gttaaaacca aaaacagtgt tatgggtgga ttctctgtat
     5041 ccggcgttaa ttgttaactg gttaacgtca cctggaagca ccaggcaccg catcacaaaa
     5101 ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac gtctgaaggt
     5161 tgttttgaaa ttggtgtcac tatcagtaac cctgtattta ctgaagatgc cattaacaag
     5221 agaaaacacg aacgggagct attaaataaa atatgcattc tttcaatgtt ggcccgttta
     5281 cgtccgatac aaaaaggatg ctggcaatga atacagcatt tgcacttgtt ctgacagttt
     5341 ttcttgtttc cggagagcca gttgatattg cagtcagtgt tcacaggaca atgcaggagt
     5401 gtgtgactgc agcaaccgaa cagaaaattc ccggtaactg ttacccggtc gataaagtta
     5461 ttcaccagga taataacgaa atcccggcag gtctttaaaa cagttccgta ataaacatcc
     5521 gatttcattc ttatatgcca gcaatggcag ggatttgttc acccttaaat ctgtaatgag
     5581 gtaaaacaaa atgagtaaag tctttatttg cgccgccatt ccggacgaac aggcaataaa
     5641 ggaagaaggt gccgtcgctg tagccactgc cattgaagcc ggtgatgaac gtcgcgcccg
     5701 cgcaaaattt cactggcaat tcctggaaca ttatccggct gctcaggact gcgcttataa
     5761 atttcttgtt tgcgaggata aacccggtat accccgccct gccctcgatt cctgggatgc
     5821 tgaatatatg caggaaaacc gctgggatga ggcgtctgct tcctttgtcc cggtcgagac
     5881 tgaatccgat ccgatgaacg tcacttttga caacctagcc cctgaagtac agaacgctgt
     5941 catggttaag ttccacacat gtgaaaacat cactgttgat atggtgatta gcgcacagga
     6001 attgttgcag gaagacatgg caacattcga cggccatatc gttgaagcgt tgatgaaaat
     6061 gccagaagtt aacgctatgt atccggagct taagctgcat gccatcgggt gggttaagca
     6121 taaatgtaag ccaagtgcca aatggcccga aattcaggca gagatgcgca tctggaaaaa
     6181 acgtcgcgaa ggtgaacgca aggaagccgg aaaatacacg tctgttgttg atctcgcccg
     6241 cgccagagcc aatcaacagc acactgaaaa ttcaacagga aaaatcaacc cggtcattgc
     6301 tgccattcat cgcgaataca agcagacatg gaaaacactg gatgacgaac tggcctacgc
     6361 tctctggcct ggtgatgtag atgccggaaa cattgacggc agcatccatc gctgggcaaa
     6421 aaatgaagtt atcgacaacg accgcgaaga ctggaagcgt atctcggcat caatgcgcaa
     6481 acagcctgat gcccttcgct acgaccgcca gactattttt ggccttgtcc gtgaacgtcc
     6541 gatcgacatt cacaaagacc ctgtggcact gaacaaatac attactgaat acctgactac
     6601 aaagggcgtg tttgaagatg aaggaacaaa tcagagcgca actgatactc tctcgtcgcc
     6661 agtaccagaa actgatgcag tggaaacggc aattccagac aacgaaaaaa ccgaatgcaa
     6721 agtggaagtc gaaccatctg tagaacgtga ggggccgttc tacttcctct tcaccgacaa
     6781 ggatggcgaa aaatacggtc gcgcaaacaa actttctggt ctggataagg cgctgtctgc
     6841 tggggctact gaaatcacga aagaagaata tttcgcccgc aaaaacggta catactcagg
     6901 ttcacaacaa aatactggtg catctgacac gaccgcacaa ccagggtcag taaaagttac
     6961 cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg acgccgatga
     7021 actgcttgca gtatcacgtg gtgaatttgt tgaagggatt agcgacccga atgatccgaa
     7081 atgggttaag gggatccaga cccgcgattc tgtgaaccag aaccagcaag aaacggaaca
     7141 gaacgaccag aaagcggaac aaaacagccc aaatacgcaa caaaacgagc cagaaacgaa
     7201 acaacctgaa ccagtagtgc aacaggaacc ggaaaagatc tgcaccgcct gcggtcatag
     7261 cggtggcggc aactgccctg attgtggtgc ggtgatgggt gacgcaacat accaggaaat
     7321 attcgatgga gagaatcagc ctgaagttca ggaaaatgat ccggaggaaa tggaaggcac
     7381 tgcgcatcag cacaaggaga acactggcgg caatcagcat catgccagcg atagtgaaac
     7441 tggcgagacg tcagatccct taattaaggc gaacggtcat cataatctca catccaccag
     7501 cagagcgggg attcatctga tgatcgacct tgaaaccatg ggaaaaaatc ccgatgcccc
     7561 gattatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg gaccggaatt
     7621 tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcgggaca ccattaaatg
     7681 gtggcttaaa caatcacgcg aagcgcaatc tgccattatg accgatgaaa tcccgttaga
     7741 tgatgcactg ttacaattgc gggaatttat cgacgaaaac tccggtgaat tttttgttca
     7801 tgtctgggga aatggagcca acttcgacaa cacgattttg cgccgttcat acgaacggca
     7861 gggaatcccc tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa tcgttgagct
     7921 ggggaaagcc atagacttcg atgccagaac ggctattcca ttcgaaggtg agcgccataa
     7981 tgcacttgat gacgcccgtt accaggcaaa atacgtttca gctatctggc aaaaactgat
     8041 cccgagtcag gctgattctt aatgttcaac tgtcgccggt tgtgactggt attctgcaac
     8101 cggcgctcgt ctgatgtaag agataaagaa atcgatgagc gaagtaatca tgattgtctc
     8161 tcccggcaaa tgggtatccg aagagcagtt aattgcgctg aaaggaataa aaaaaggtac
     8221 gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca
     8281 tgacggtatg ccatgggata acagtccatg cttttacaac ctggaagaaa ttgatcgctg
     8341 gattgagcgc caggcatcag caagaccaag acgtcatctt acttgactaa aagccacact
     8401 aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat
     8461 tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaacctgggg gttcctgaca
     8521 cagcaaaaaa caggcgcgtt gcaggtgagc tacgcgcctc tgtttgttac gcaataaaaa
     8581 ctggtgtttt cgactatgca aaacagtttc cctcctcacg caatctggaa aaatttggtg
     8641 aggcccgaca agatttaacc ataaaagaac tggctgaaaa atttctggca ctgaaagaaa
     8701 ctgaagtcgc aaaaacatca ctcaacacat accgtgccgt catcaaaaat atcctgagca
     8761 taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg gaggttcgta
     8821 aagagctact gactggatac cagatcccca aaagtaacta tattgttaca caaccaggga
     8881 gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc cagtttggtg
     8941 ttgataacgg ttacctggta gataacccgt ttaaggggat ctcgccatta aaggaatcaa
     9001 gaaccattcc ggatcctctt tcgcgggagg aattcatccg tcttatcgat gcgtgcagaa
     9061 atcagcaagc caaaaattta tggtgtgttt ctgtttatac tggcgttcgc cctggtgagc
     9121 tgtgtgcact tggatgggag gacatagatc tgaaaaatgg aacaatgatg atcaggagaa
     9181 atttagcaaa agaccgtttc acggtaccaa aaacacaggc gggaaccaat cgggtcattc
     9241 atcttattaa gccagcaatc gacgctctcc ggagtcagat gacattaacg agactgagca
     9301 aagagcatat cattgatgtt cacctcagag agtatggcag aacagaaaaa caaaaatgca
     9361 cctttgtttt tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac cattttaccg
     9421 ttgactcaat aaggcagatg tgggacgcag cgataaaacg tgccggactc cgccatcgaa
     9481 aatcatatca gtcgagacat acttatgcct gctggtcgct gacagctggt gctaacccgg
     9541 catttatagc aaaccagatg ggccatgcag atgcgcaaat ggtatttcag gtatacggaa
     9601 aatggatgtc tgaaaacaat aatgcacagg tagctttgtt aaatacacag ttaagcgagt
     9661 ttgccccaac catgccccat aacgaagcaa tgaaaaatta atttaatatt tatcaaatag
     9721 ttaacactca tgactcttga aatccataaa ttcaagcgca gtgcccagcc atcccgatac
     9781 tgctgctttc accaaatcct tagtgcttct ttcgtgtttt tctattgtca taatggttat
     9841 ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa
     9901 agtgagtaag actttgcagc aatgcttttg atcctgttca aataaactaa tggcatcagc
     9961 aacatgctgg aaatcaaacg tatgggtaat tagtttttct ggtttaatta accctttact
    10021 taaccagtcg ataacgaccg ggaatttatt tgcatttaag cgtgaagaga aaatagagag
    10081 ttcttttccg gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa
    10141 tacaatacgt gccgctggag aagccagcgt tacggcttct ttcaggattg aaggatgaca
    10201 agccgcatcg ataattaatg tcggctttat gcctttttca gcgaaaatct cgccaagcgg
    10261 tgtctggctg ttattaatcg cccagtctgc cccgctctct ttcgcttttt ccagtcgttc
    10321 atcaatgcga tcggcaacaa tcacattctt aacgttatag acgcctttta atacctgaac
    10381 gatcgtcagg ccgattggac cggcaccgta aaccagaacg gtatcatttt cagtcggttg
    10441 accatgaccg gtaacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc
    10501 ggccactgct tcaggaattt tccacgcatt ttttgccggc accacggcat attcactgaa
    10561 accaccgtca gcgtgcacac ctaatacagc cagtgtcgta caaacgttcg gcttacctat
    10621 agagcacgga tagcaatgcc cacagctgac caccggatcg acagcaacgc gttcaccgac
    10681 tctggcgctt tccacgcctt cacccactgc atcaatgacg ccaaagaatt catgaccaat
    10741 gacgcgcgga tatttcgcaa aaggattatg cccacgataa atatggctat ctgaaccaca
    10801 aattccggca agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcgcg
    10861 ttcgataatt gccagttgat tcggtttttc aattaaaatg cttttcatta tcttactcct
    10921 taccagttcc acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag
    10981 ggatatttcg ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga
    11041 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcaagcat ttgttcggaa
    11101 taacccatgt attcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
    11161 gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacaac
    11221 gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg
    11281 cggatataat cgataagttg ctcttcaatc agttgcttgc agtcccagat gctgttgaag
    11341 acttcaccca ccgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcctgg
    11401 ttttccgcag gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
    11461 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
    11521 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
    11581 gtcgaccaca actgctcttc cggccactgt cctttggttg cgggttcata agccagacct
    11641 ttgcctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg
    11701 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
    11761 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
    11821 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
    11881 gccgacatcg tcaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
    11941 tcttcgatac ggtgtgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa
    12001 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
    12061 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
    12121 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
    12181 ctgagctact accatacaag tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg
    12241 cgtatatttg cgccgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
    12301 cacttccttg tatcttattg ataaaatgga tttatgtcct tacgcgcgcc cccagcccgc
    12361 aacaatgatc aacatgccgc aaagcgcaat caacgccccc gtccagtcat aaagactcag
    12421 tttcacgcca tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata
    12481 agccgcgtaa acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagtgc
    12541 cagagaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
    12601 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
    12661 aatcatcttt gtcacttatt gacatcatgt atagttatag gacgacataa tatcatcaat
    12721 ataaacatcc tcctggtacg ttttgcgttc gcagtggatg gtgtagaatc acctttaatc
    12781 attcatacag ggaatgaatt atgaaaatta ctctcagcaa acgaattggc ctgctcgctt
    12841 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
    12901 tgattgagtc tggcgacagt gcgcaaagcc gccagcacgc cgctatggaa aaagagcaat
    12961 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
    13021 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
    13081 actgggagcc aaatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
    13141 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag
    13201 ctacgcccgc tggagcgtga agatttacgc tatgtacatc aactcgacaa taacgccagt
    13261 gtgatgcgtt actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtac
    13321 gataagcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
    13381 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata
    13441 attatctccc cggaatatca ggggaaaggt ctggcaacgc gtgccgcaaa attagcaatg
    13501 gactatggct tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat
    13561 gaaaaagcga tacacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat
    13621 gagttcttta ttaatggtca atatcggaat gccattcgca tgtgcatatt ccagcatcag
    13681 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
    13741 taataataat cgatagtatt tttgatggtg taaacccgtt cgatggcggg ttttactcct
    13801 tcatcaacaa tgattagctg acagtccacc ggattggcgt gactgtcata ttcacagctc
    13861 tgttttacat taccaactct ttgattattc agtatagtaa ccgctgtgta atctaatttt
    13921 ttgatcgggt ccgttgatgg cgtggcgcta acagataatg ttttgtcgtg acttttcgtg
    13981 gttttaccca gcggataccc ctgatcatca tagcgatatt ccatctgcat ctgtttgctg
    14041 ctggctttaa tcacaaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat
    14101 tctgctagct ggcattttcc ctgcaaacga actcttttct ccagtgtctg ggcatcacgg
    14161 taataattgg cgtccagtac cagggcaacc acggtattat tttccagatc cagtaattcg
    14221 agtgaatcaa aacagccttc ttccgacaaa gtccccgaaa cacgtttcgt cacttcacct
    14281 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
    14341 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgttcggtca
    14401 catcctgtga ggaatatcgc gagtaagcat ggcagtaatt tgtatttcac aacagtcacc
    14461 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
    14521 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
    14581 cgcgcttctg ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cctgtgagcg
    14641 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
    14701 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg
    14761 cgctaatgat acgcataaaa ttctctacac ccgcaccacc agcggtaacg tctctgctcc
    14821 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taacacatta tcgactgaac
    14881 gccggatacg acacatccgg cgatttgaac atacaacata atcccacctt attactcata
    14941 cccttctatt gatatggatt aataattctt aacccaaaat gggtagactc cctctattgt
    15001 tagcgcgcta aatattcaat atataaactt ttatataacg ataaagatca gggagtgagt
    15061 tatgtccaaa aatgaacaaa tggtgggcat cagccgcaga acactcgtta aatctaccgc
    15121 gataggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc
    15181 agcggcagcg gtacagcagg ccagcgagaa agtggtctgg ggtgcctgtt ccgtcaactg
    15241 tggtagccgc tgtgcacttc gtctgcatgt taaagataat gaagtgacct gggtggaaac
    15301 tgacaacacc ggcaacgacg agtatggcaa tcatcaagta cgtgcctgtt tgcgcggtcg
    15361 ctccatccgt cggcgtatta atcatcccga tcgcttgaat tacccaatga aacgcgtggg
    15421 caaacgcggc gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc
    15481 cagtagcctg aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc
    15541 ttcagggatc gttggcggca atatgacccg ctcttcgcca tccgcctcgg cggtcaaacg
    15601 cctgatgaac tgctacggcg gttcactcaa ccagtatggc tcctacagca ccgcgcaaat
    15661 ttcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca cggatattga
    15721 aaacagcaag ctggtcgtga tgtttggtaa caacccggca gaaacccgca tgagcggcgg
    15781 tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg aacgcaaaaa tgattgttat
    15841 cgatccgcga tataccgata cagctgctgg tcgtgaagac gaatggctac ctattcgccc
    15901 gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg aaaatctcgt
    15961 tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct tacccgcaga
    16021 tgcaccaaaa aatggtcact ataaagccta tattcttggt gaaggagacg acaaaacagc
    16081 gaaaacgccg cagtgggctt cgcaaattac cggtatcccg gtggaccgta tcatcaaact
    16141 ggcgcgtgaa attggcacag caaaacccgc ttatatctgc cagggctggg gaccgcaacg
    16201 ccaggcaaac ggcgagctga ctgcacgcgc tattgcgatg ttgcctattc tgacgggcaa
    16261 tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg acctatacca ttaccataga
    16321 acgcctaccg gtgctggata atccggtcaa aacgtcaatc tcctgcttca gctggacaga
    16381 tgctatcgat catggcccgc aaatgacggc aatccgcgac ggtgtccggg gcaaagataa
    16441 actggatgtg cccattaagt tcatctggaa ctacgcggga aataccctcg ttaatcagca
    16501 ttctgacatc aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg aaatgattgt
    16561 ggtcatcgaa aactttatga cctcatcggc aaaatatgcc gacattctgc tgccagacct
    16621 gatgaccgtt gagcaggaag atattattcc taacgactac gccggtaaca tgggatatct
    16681 cattttcctc cagcctgtca ccagcgaaaa attcgaacgc aaaccgattt actggatcct
    16741 gagtgaagtc gcgaaacgtc ttggacctga agtctatcaa aagttcactg aaggtcgcac
    16801 tcaggaacaa tggttacaac atctgtacgc caaaatgctt gccaaagatc cggcgttacc
    16861 gtcttacgac gaattaaaaa aaatgggtat ctataagcgt aaagatccca atggtcattt
    16921 tgtcgcctac aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa cgccttccgg
    16981 taagattgaa atttactcca gcaagctggc ggaaattgcc cgtacctggg aactggaaaa
    17041 agatgaagtg ataagcccac tgccggttta tgcctcaacc tttgaaggct gggactcccc
    17101 tgaacgtagt acctttccac tgcaactgtt tggtttccat tacaaatccc gtactcactc
    17161 gacctacggc aacattgatg tcctgaaggc agcctgccgt caggaagtgt ggatcaaccc
    17221 tatagatgca cagaaacgtg ggattgccaa cggcgatatg gtgcgggtgt ttaaccatcg
    17281 tggcgaagtt cggctaccag ccaaagtgac accacgcatt ctccctgggg ttagcgctat
    17341 gggccaggga gcctggcacg aggccaatat gtctggcgat aaaatcgacc atggcggctg
    17401 tgtgaatacg ctaaccactc tgcgcccttc accactggcg aagggaaacc cgcagcacac
    17461 taatctggtc gagatcgaaa aaatataacc cactacaacc ataaattctg acatgacatt
    17521 ttgtttgaaa agcaataagt gagtaatgat gaaaatccat accacagagg cgctcatgaa
    17581 ggctgaaatc agccgtagaa gtctgatgaa aacctccgca cttggcagtc tcgcgctggc
    17641 aagcagtgct ttcactctgc cattttccca aatggtccga gcggcagagg taccggtaga
    17701 agagaaagcg gtctggagtt cctgcaccgt taactgcggg agccgctgtc tgttacgttt
    17761 gcatgtgaaa gatgacaccg tgtactgggt ggagtctgat acgacaggtg acgacgtcta
    17821 cggtaatcat caggttcggg cgtgtttacg cggacgctct atccgtcgac ggatgaatca
    17881 tcctgatagg ttgaaatatc ccatgaagcg tgtcggcaag cgcggtgaag gtaaatttga
    17941 acggataagt tgggacgaag ccctggatac catcagtgat aatcttcggc ggatcctaaa
    18001 agattacggc aatgaggctg tacatgttct ctatggaaca ggcgtagatg gcggaaacat
    18061 caccaactca aacgtcccgt accgtctgat gaactcttgc ggtggttttc tcagtcgcta
    18121 tggcagctac agtaccgcac agatcagtgc cgcaatgagt tatatgttcg gtgccaatga
    18181 tggcaacagc ccggatgata tcgccaatac gaaactggtc gttatgttcg gaaataaccc
    18241 ggcagaaact cggatgagcg gcggtggtgt cacttactac gtcgagcaag cccgagaacg
    18301 ttcaaatgca cgcatgatcg tcatcgatcc acgttataac gacacagctg ccggacgtga
    18361 agatgaatgg ctgcccattc gccctggcac cgatggagca ctggcctgtg cgattgcctg
    18421 ggtactgatt actgaaaaca tggtcgatca gccatttctc gacaaatatt gtgttggtta
    18481 cgatgaaaaa acgctgcccg ccaacgcacc acgtaacgcg cattataaag cctatattct
    18541 gggcgaaggg cctgacggca tagctaaaac gccggaatgg gcagcaaaaa tcaccagcat
    18601 accggcagaa aaaattatcc atttggcacg agagatcggt tcagcaaaac ctgcttatat
    18661 ttgtcagggt tgggggccac aacgacactc caacggcgaa caaacgtccc gcgctattgc
    18721 catgctttcc gttctcaccg gtaacgtcgg cataaacggc ggcaactcag gtgtacgcga
    18781 aggtagctgg gatctggggg tagaatggtt cccgatgctt gagaatcctg ttaaaacgca
    18841 gatttccgtc tttacatgga cagatgccat cgaccacggt acggaaatga ccgcgacccg
    18901 tgatggtgtt cgtggaaaag aaaaactgga tgtccccatc aagtttttat ggtgctacgc
    18961 cagtaacaca ttgatcaatc aacatggcga catcaatcac acccatgagg tgcttcagga
    19021 tgacagcaag tgcgagatga ttgttggcat tgaccacttc atgactgcct cggctaagta
    19081 ttgcgatatc ctgttgcccg acctgatgcc aacagagcaa gaagacctta tctctcacga
    19141 atctgccggg aatatgggct atgtgatcct cgcccaaccc gcaacctcag caaaatttga
    19201 acgcaaaccc atctactgga tgctgagtga agttgccaaa cgcttaggac cagacgttta
    19261 tcaaaccttt actgaaggtc gcagtcagca tgaatggatc aaatatctcc atgcgaaaac
    19321 gaaggaacgt aaccctgaga tgcccgacta cgaggagatg aaaacgaccg ggatctttaa
    19381 gaaaaaatgc ccggaagaac actacgtcgc tttccgcgca ttccgtgaag atccacaggc
    19441 aaacccgttg aaaacacctt cggggaaaat cgaaatttat tctgaacgac tggcgacgat
    19501 tgcggatacc tgggaattga aaaaagatga aattattcat ccccttcctg cgtatacccc
    19561 aggttttgat ggctgggacg atcccctgcg gaaaacctat ccactgcagt taacgggctt
    19621 ccactacaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac agcaggcctg
    19681 cccacaagag gtgtggatca accctattga tgctcaggca cgcggtatcc gtcatggcga
    19741 taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag tgactccgcg
    19801 tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg atatgtttgg
    19861 tgaccgggtc gatcacggcg gcagtatcaa tattctgacc tctcaccgcc cttcaccgct
    19921 ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt aaggagtagc
    19981 ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc tgtaaaacct
    20041 gcgaactggc gtgcaaagat tttaaagatc ttggcccgga agtcagtttc cgccgtattt
    20101 atgaatatgc tggcggcgac tggcaggaag ataatggcgt ctggcaccag aacgtgttcg
    20161 cttattatct ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca aaagtctgcc
    20221 ccagcggcgc aatgcataag cgtgaagatg gctttgtggt ggttgatgaa gatgtttgta
    20281 tcggctgccg ctactgccac atggcttgtc cgtacggcgc gccacagtac aatgctgaaa
    20341 aaggacacat gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg aaacaaccca
    20401 tatgtgtcga atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa gagctgcgcc
    20461 agaagcacgg cactctggct gccgtcgcgc cactaccgcg cgcgcatttc acaaaaccca
    20521 atatcgttat caaacctaac gccaacagcc gcccgaccgg cgataccact ggttatctgg
    20581 ctaatccgga ggaagtgtaa aatgggaaat ggatggcatg aatggccact ggttatcttt
    20641 accgtattgg gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg ctggttcgcc
    20701 gccaataatg acgcagaccg tcagcgtatt gtccgtggca tgtttttcct ctggctgttg
    20761 atgggcatcg ggtttattgc ctctgtcatg cacctgggat cgccactgcg agcgtttaac
    20821 tcgctgaatc gaatcggagc ttctggtctg agtaacgaga ttgctgccgg ttccatattt
    20881 tttgccgttg gcggattatg gtggctggtg gcagtcatcg gtaagatgcc gcaagcgttg
    20941 gggaaactct ggttacttgt cagcatggcg ctcggcgtca ttttcgtctg gatgatgacc
    21001 tgcgtgtatc aaatcgacac agtgccaacc tggcataacg gttacactac gctggctttc
    21061 ttcctgacag tattgttgag cggtccaata cttgctgccg caattctgcg ggcagcccgc
    21121 attacattta ataccacacc ctttgccatc attagcgttc tggcgctgat cgcttgtgcc
    21181 ggggtgattg tgctgcaagg tttgtctctg gcgtctattc actcatccgt acaacaagcc
    21241 agcgcactgg taccagatta cgcctcctta caggtctggc gtgtggtatt gttatgcgca
    21301 ggtctaggat gctggctttg tccgctcatt cgtcgccgtg aacctcacgt tgccggactt
    21361 gttctggggc taatcctcat acttggcggt gagatgatcg ggcgtgtgct cttttatggc
    21421 ctgcacatga cggtaggaat ggctattgca ggttaacaaa ttggtgcggg gcaacccgca
    21481 catttcagga tgttaggaat gacccatttt tcacagcaag ataatttttc tgtcgcggcg
    21541 cgcgtgttgg gcgcgctgtt ttattacgct ccagagagtg cggaagccgc acctctggtt
    21601 gcggtactca cccgtgacgg ctgggaaact cagtggcctc taccagaagc gtcattagcg
    21661 ccgctggtga cggcttttca ggcacagagt gaagagactc gcgcccaggc ctggcagcgt
    21721 ttgtttgtcg gtccgtgggc tctgccatca ccgccgtggg ggtctgtctg gctggatcgc
    21781 gaatctgtgc tgtttggcga ttcaacattg gcacttcgtc agtggatgcg cgagaaaggc
    21841 attcagtttg aaatgaagca aaacgaaccg gaagatcatt ttggttcact gctgttgatg
    21901 gctgcgtggt tggcggagaa tggtcgccag acagaatgcg aagaattgct ggcatggcac
    21961 ctttttccgt ggtcaacacg ttttcttgac gtttttatcg aaaaagcaga acaccctttc
    22021 taccgtgcac tgggtgaact ggctcgctta acgctggcgc aatggcaatc acaactgtta
    22081 attcctgtcg cggttaaacc actctttcga taggatcacc gtaatattgc cggatggtgt
    22141 cgattaggcg caattcggca ataataggtt acagtgtcac gttttttatc tcttaaagca
    22201 cgcactgctt ttgcgtctgg cttcttttgc cgcaaaatag tcgcccgtat ttcattgccc
    22261 atttctgctc atgcatcatc tacacatcta tccggatctg cgcactatgt tccgccgtct
    22321 gcttatcgca acagtcgtcg gtattcttgc ggcctttgcc gttgccgggt ttcgtcatgc
    22381 aatgctgcta ctggagtggt tgttcctcaa taatgactcg ggcagtctgg tcaatgcagc
    22441 gacaaatctt tccccctggc gacggctgct aactccggcg ctcggcggac tggcggcggg
    22501 tttgttgctg atgggctggc agaaatttac ccagcaacgc cctcatgcgc cgaccgatta
    22561 catggaagcg ttgcaaaccg atgggcagtt tgattacgca gcaagcctgg ttaaatccct
    22621 tgcctctctg ctggtagtaa ccagcggcag tgcaattggt cgcgaaggtg cgatgattct
    22681 tttagctgcc cttgccgcct cctgttttgc ccaacgtttt acgccacgcc aggagtggaa
    22741 attatggatc gcctgtggag ctgcggcggg aatggctgcg gcctatcgcg ccccgcttgc
    22801 tggcagttta tttatagccg aagtgctgtt tggcactatg atgttggcct ctctcggccc
    22861 ggtgattatt tccgccgtcg tggcattgct ggttagcaat ctgattaatc atagcgacgc
    22921 gttactctac agtgtacaac tctcagtgac ggttcaggct cgtgactatg cgctgattat
    22981 cagtacaggt gtgctggcag gtctgtgcgg accactgttg ttaacgttga tgaacgcctg
    23041 tcatcgtgaa tttgtaagtc tcaagcttgc gccgccctgg caactggcac tgggcgggtt
    23101 gatcgtgggt ctgctttccc tgttcacacc tgcagtgtgg ggcaacggct atagcaccgt
    23161 acaatccttt ttaaccgctc cgccactgtt aatgatcatt gccgggatct tcctctgtaa
    23221 actgtttgcc gtgctggcga gtagtggttc tggcgcgccc ggtggtgtct ttacaccgac
    23281 gctatttatc ggtcttgcca ttggcatgtt gtatggtcgt agcctgggat tatggttccc
    23341 tgatggcgaa gaaattacgc ttttactcgg attgaccggg atggcgacac tgttggcggc
    23401 aaccacgcac gcgccgatta tgtcgacgtt gatgatatgt gaaatgaccg gggagtatca
    23461 gctactcccc ggtttattga ttgcctgcgt aattgcgtcg gtaatttcgc ggacgttaca
    23521 ccgtgactct atctaccgcc agcacactgc gaagcatagc taagcggatg tattgcccca
    23581 gttcacgctg ttcggcgcgc ggcagataag gcagttcacc aatgagcggt gccggaagtt
    23641 ttttaccgag cacatcaatg atttccgcat aatgcgccag tcctggattg attcggttag
    23701 ccacccagcc aatgagcggt agcccgtcgt tggcgatcgc ctgagctgtt agcagtgcat
    23761 ggttaatgca accttcctga ataccgacaa ccatcaacac cggcagttgt tcctgcacca
    23821 cccattcaga gagcggacgc aaatcattca tcagactgcg ccagccgcca gtcccttcta
    23881 ccacgacatg atcgacttta tcggtcaggt ttgccaggcc gtttgaaatg agggtgtaat
    23941 tgattgggca actgtgcgcc acgctacttt cttcttcgct taacgcgata ggattaactg
    24001 cttcataagg cagttcgatg gttgaaacac tctgcaacac cagggcatct ttattacgca
    24061 gcccttcggg tgtctctttg ctccccttcg ctacgggttt atatcccgca accgtttttc
    24121 cctgggaggc taacgcttgt agcaatgcgc gggaaaccac cgttttccct acagaagtgt
    24181 ctgtaccggt aataaagaaa cgcttcagca tcactaactc caccgttatg catcacaaat
    24241 ataaaccagg aaaataatta accttgaaag tctaagttat gctttcctgg cccaaattga
    24301 gatagcgcaa attttggtag aacagttaaa aaatgttaac cctgcaacag acgaatcaac
    24361 aaagaaccgt tatacatcgc gtcttttacc agcgcagccc ctgccatcgt gccctggtta
    24421 gaaaactgag tactctcaac gctaatgtgc tgactatacg caggaagggc ctgctgacgg
    24481 atgctatctg agatgaccgg gaagaggata tctgccgctt tacttaacgg tgagccaatc
    24541 agtatttttt gtgggttaaa taaattcacc atgatggcaa gaatgcgccc tacatgcgcg
    24601 cccaccccgg taatgatgtc ttttgccagc agatcgccgc gcaatgccgc ctgacacaat
    24661 gagtccacgg ttaacggttg tccatgtaac atcgagctca tggattgatt gagacgcagc
    24721 tgtgccagct caagaatact gtccacactg gcgatggttt cgaggcagcc gtgattcccg
    24781 caataacagc gtttcccata cgggtcgacc tgcgtgtgac ctatttccac aaggctactg
    24841 ctgcctgcgt gtagcagatg accatcggta atgacgcccg cccccacgtt gtgatcgata
    24901 accacctgaa tcacatcgcg cgccccgcgt gaggcaccaa acaaggcctc agccatcgtc
    24961 catgcgctga tatcatgctg aatataaacc ggtacgccgg tatgctgctc cagcgcctcg
    25021 ccgagcggca tctcttttac atcctcgtag aacggcatgc gatgtacaat accattttcc
    25081 gtatcaataa ttcccggcaa ggtaatggca atcgaagtca gacgctcaag ttttttctgg
    25141 tggcggataa aaaactgatc gatatgggaa atgatacgat ccagcaatgg tgagtcatct
    25201 tttaacgcca gttcctgcgc ctcctccacc accagtttgc tgctcagatc gcgcagagca
    25261 aggaaaatct ccccgcgact aatgcgcaga gaaagatagt gccaggcttc agtttcaacc
    25321 accagcccca ctgccggacg gccacggttc cccgcttctt tgatttccag ctcttgcacc
    25381 aggtgtgctt cgagcatctc acggacaatt ttagtgatac tggcaggagc cagctgcgcc
    25441 agacgggaaa gatcgatacg cgagactgga ccaagctgat caatcaggcg ataaaccgcg
    25501 cccgcgttgg tctgctttat ttgatcaatg tgcccaggct gattttcagc aaccaccgca
    25561 tactccctat attttcgcgc tccgaaataa tctgtaggct atggtgaagc acttcaatac
    25621 gtgtcgtcaa atttttactt aggcatgtga ttaacagcac atttttcggg ctttttcgct
    25681 gaaatttccc tcacctgaga gcattcagca gatgaatacg aaagttacgc gcagccggac
    25741 tttgttcatg gtgtttcggc cagaccaacc acatttcaga aaccgcatcc tcttcagcaa
    25801 tcggcaccca gcgcatttcg ttgagctgaa cacgtttaaa tgacgcaggc aaaattgaaa
    25861 cacccagacc agcggaaacc agaccgatga tggtcattgc ctcacccacc tcctgagtga
    25921 tgacgggcgt caaattgtaa cgtcgcatca gcccgagaat atcgtcatac agccctgtcc
    25981 ctacgtgcgg atcaaaaaag acaaagggtt ctttcgccag ttcagccagc gttacactcg
    26041 ggttatttgc caggggatga tcgtgcggga tcatcgccat aagcggttca tggacgatga
    26101 ctgcgtgttc aagcgtctcc ggtaacgctg tgttacgcag caatcccata tccagcgttc
    26161 cttcaatgag cggagcgatt tgctcgcgag tgttcatttc gcgggtttgt aaatggacat
    26221 cgggataatc acggcgaaac agcgataacg tatcggacac cgcccgaata aaaggagcgg
    26281 acgaagtaaa accaatgcgc aactcccccg cttcgccctg atgcagcctt tcagcgcgag
    26341 cggcagcatc atccaccata gacaagattt gccgactatc tacaagaaac tgttttcctg
    26401 ctgccgtcag caatacactg cgattggtcc gtgccagcag tcgggcacca atttgttgct
    26461 ccagcgcctg aatctgttga cttagcggtg gttgcgaaat attcaggcgg gcagcggcgc
    26521 gcccgaaatg cagctcttct gcaaccgcaa caaagtaacg cagatgacga agttcaatat
    26581 tcatatttaa aacatcttat ttgagattat taatatatta gacagaacaa ttcgattttc
    26641 ctaatctatt cataagcctg atccacaggc atatttagca aggatttcaa gtgagccgta
    26701 ctacaactgt tgatggagct ccggcaagcg acactgacaa gcaaagcatt tctcagccaa
    26761 atcaatttat taaacgcggt acgccgcaat ttatgcgtgt caccctggcg cttttctctg
    26821 ccggactggc aaccttcgca cttctctatt gtgtgcaacc tatccttccg gtgctttcgc
    26881 aggagtttgg cttaacgcct gctaacagta gtatttcact gtccatttca acggcgatgt
    26941 tggctatcgg tttgctgttt actggcccgc tatccgatgc cattggtcgc aaaccagtga
    27001 tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg atgaccagct
    27061 ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc gtggcagctg
    27121 ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt tcgatggggt
    27181 tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagc ggtgtcttca
    27241 cggacttttt caactggcga attgcgctgg cggcaatcgg ttgtttcgcg ctggcctcgg
    27301 cgttaatgtt ctggaaaatc ctccctgaat cacgccattt tcgcccgact tcgctgcgac
    27361 ctaagacgtt gtttatcaac tttcgtctgc actggcgtga ccggggatta ccgttattgt
    27421 tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac atcggctatc
    27481 ggttgatgct ctccccctgg catgtcagcc aggccgtggt tggcttatta tcgctggctt
    27541 atttgaccgg tacatggagc tcacccaaag ccggaacaat gaccacccgc tatgggcgcg
    27601 gtccggtgat gttgttttcg acgggggtta tgctgtttgg tttactgatg accttattta
    27661 gctcgctgtg gctgatcttt gccggaatgt tattcttctc agccggattc ttcgcagccc
    27721 actccgttgc cagcagctgg atcggccccc gcgcaaaacg cgctaaaggc caggcctcct
    27781 cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg ggtggtgttt
    27841 tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg ctggtcattg
    27901 ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc ctgaaaaata agtccggact
    27961 gcggtaaata cccatccgga cttattgcct gctcaaacca acgttaatag ccatcctaaa
    28021 atagacgaag cgccagccaa ttcccgcagc gcatctagcg tcatcaggac tataagtacc
    28081 caaataaacg gattcatttt gctgtgtgtc atttattact gatgcgcagt tattctaccg
    28141 ctttgtaagt agtaaaatag ttaacctgat caagactact attattggta gctaaatttc
    28201 ccttaagtca caatacgtta ttatcaacgc tgtaatttat tcagcgtttg tacatatcgt
    28261 tacacgctga aaccaaccac tcacggaagt ctgccattcc cagggatata gttatttcaa
    28321 cggccccgca gtggggttaa atgaaaaaac aaattgaggg tatgacaatg aaaaaagtat
    28381 tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct gcagagactg
    28441 cgaccacacc tgctccgact gcgacgacca ccaaagcagc gcctgcgaaa accacacatc
    28501 ataaaaaaca gcataaagca gcacctgccc agaaagcgca ggcggctaaa aagcatcata
    28561 aaaatgcgaa aactgaacag aaagcccctg aacaaaaagc gcaggcagcg aagaaacacg
    28621 ccaagaaaca cagccatcag caaccggcaa aacctgctgc acaacccgca gcgtaagttt
    28681 tcaacagtaa tactggcgcg ccccctcgcg cctgaaaatt acggtgctaa gcgggtaacg
    28741 tttagcaccg cctttagcgg agggtagtat ggtgggccgt tatcgctttg agttcattct
    28801 tatcatcctt attttatgcg cactgattgc cacccgtttt tatctttcct gatcgtagtt
    28861 atctgatttt actcccactt tcatcccgtc ccgtctatag tatttatgag ggtttgcttt
    28921 taataatcat aattacccac cagagtgtga tatgcgtaca accattgctg tagtgttggg
    28981 tgcaattagt ttgacgtctg cttttgtgtt tgcagataaa ccagacgttg ccaaatcggc
    29041 aaacgatgag gtcagcaccc tgttttttgg tcatgatgat cgtgtgccag tgaatgacac
    29101 gacccaatca ccgtgggatg cggttggcca actggaaacg gccagcggca atttatgtac
    29161 ggcgacgctg attgcaccca atctggcatt aacggcagga cactgtttat tgacgccgcc
    29221 aaagggtaaa gccgataaag cggtagcgct gcgtttcgtg tcaaataaag gtctttggcg
    29281 ctatgagatc cacgacatag aaggccgcgt tgatccgaca ctgggaaagc ggttaaaagc
    29341 agatggggat ggttggattg ttcctcccgc agctgcgccg tgggacttcg gtttgattgt
    29401 gctacgtaat cccccttctg gcattacacc gttgccgtta tttgagggag ataaagccgc
    29461 gcttaccgcc gcattaaaat cggctggccg taaagtgact caggcaggat accctgaaga
    29521 tcatctcgat acgttgtaca gtcatcaaaa ctgtgaagtg actggctggg cgcaaacgtc
    29581 ggtgatgtcg catcagtgcg ataccttgcc gggtgacagc ggttcgcctc tgatgttgca
    29641 taccgatgac ggctggcaat taattggggt gcaaagttcg gctcctgccg cgaaagatcg
    29701 ctggcgcgcc gataaccggg ccatttctgt taccggtttt cgcgacaggc tggatcaact
    29761 gtcgcaaaaa taatgttcaa acgctgcccg acagcgcggg cagcgtgttc atcaggcaag
    29821 tttcaccatg atcattccag ccaacagcaa gaccaggcca atccagcctt tacgatttaa
    29881 ccgttgacca aacaagatcc aacctgcggc taacgtggcg gcaataccaa acccgcccca
    29941 caatgcataa gcgacagaca agtcgatccc tttaacggcc tgagaaagcg cactaaaggc
    30001 agccagcacc gccgccaggg agagcaagcc aaatattttg cgacgaaagc cgtcagaaaa
    30061 tttcaaaaag acgttagcaa cgatttccag cacgattgcc aatgccagcc aggcggcgtg
    30121 aacccattca aactgcgcca tggttcacct ccagttcagg tttacgcgct ttacgggtac
    30181 ctgatttgat caatacaatc ccggcgacca gggtggttaa cccggcaatt ttcatcagcg
    30241 ataaactttc gtcgaataac aaaacactaa acaaggtaat aaataaaata ccgatacctt
    30301 cccacagcgc ataagccacg cctaaggcga tctttttaac ggcgaaagag agaaatatat
    30361 acgacagaga aatcatcacc agcattaaaa taaagccgcc atttccctca ctgacgctcg
    30421 cccatttcat agacagcgta ccggtaattt ctgtagcaat agccagacct aataaaatcc
    30481 aataaatata cattgtcctt ctcctgcaag agaattattt taattttcgc ttaattcagc
    30541 gaaaccaaag taaagtggtc aagccccgcg catagcgcaa gctttggcag aagaaaggac
    30601 taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga ggtacgaaga
    30661 tgggtaaatg tagaaaataa cgtcctgaac aaattgtcca taatattaca attatccgca
    30721 gtgttgcttc tcgtcatcgc ggatgataat tgtcctcggt agttgaacac gcctgatttg
    30781 tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc ggtttgattt
    30841 tcgttatgat gacggagcga aatagacatt attattagca aaggaagaaa aaacggggac
    30901 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
    30961 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
    31021 cgcattattt attgctgtta ttcttaaccc gctggtgcaa cacatggtcc gctggcgagt
    31081 gccgcgtgta ctggcggtgt cgattttgat gaccatcatc gtgatggcga tggtgttgct
    31141 gttagcttat ctgggttccg cactcaacga gttgacgcgg acgttaccgc aatatcgcaa
    31201 ctctattatg acgccgctgc aagcgcttga accgttgttg caacgcgtag ggattgacgt
    31261 ctcagttgac cagctggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
    31321 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
    31381 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
    31441 ggttgaaggg atggcggcga ttcagcgtgc gattgacagt gtgtctcatt atctggtgct
    31501 gaaaacagcc atcagcatca tcaccggcct ggtcgcctgg gcgatgctcg ccgcactcga
    31561 tgttcgcttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tcccgaatat
    31621 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
    31681 cgaagcgttg ttcgtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
    31741 ggagccacgc atcatggggc gtgggctggg gctttccaca ttggtggtat ttttgtcgtt
    31801 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
    31861 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
    31921 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
    31981 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
    32041 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
    32101 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
    32161 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
    32221 catcattgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
    32281 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacgttga
    32341 taccacgagc gcgcagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
    32401 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
    32461 cttctgcggt gatttcgcgg tgctcgccca cttcctgatc atcgccagta gaagagccgt
    32521 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgct ttacacataa
    32581 tgtaagaaag gatagcgccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
    32641 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
    32701 ccaccactgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
    32761 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
    32821 atacaatcag cagcaggaag gaaacgacca gagccgccag gttcattttg tgacggtttg
    32881 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgaac
    32941 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
    33001 ccagaatcgg tgccattccc gcgtcatgat gcagatagct gttaaagcca accagcactg
    33061 ccgccagacc cacgaagcta tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
    33121 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
    33181 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
    33241 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagcccggcc agactgaaga
    33301 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
    33361 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
    33421 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc gccctggcca
    33481 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtgac cgacatcaac
    33541 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
    33601 gcgaacacag tgaagtgccc aaggaattct ttcggcgcaa cgcttgccat ccagccaaaa
    33661 agaatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggt acatttttcc
    33721 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
    33781 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
    33841 tcatcaaaat caacagtgat gttgccatct ttctctttgc acaacagttt cagcagatta
    33901 acgaggtttg tgccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
    33961 taaccaatca ctttgacacc attttccgta gtgaagattt cacccggcac ggtgtattca
    34021 cagttaccgc cgttctgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
    34081 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
    34141 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
    34201 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctctttaaa atccagctcg
    34261 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
    34321 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccagacctgc aacacccgca
    34381 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
    34441 aagcgcccaa attcatgtgc tgcttcaaca atggcgcgat aaccggcgat gttcgccatc
    34501 gagcttagtg cgtccagcga ttgtgcgcgt gatatacgcg gcacagagtc catcgccatc
    34561 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
    34621 aaactcacca gcgttgtccc aagattcagt aacgcaattt catcatctaa cggcgcattg
    34681 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
    34741 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
    34801 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
    34861 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
    34921 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
    34981 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
    35041 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
    35101 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcaggt
    35161 aaaacgagac atcgccccag tacgaatcac tacttaacat taaattaact tatacaattc
    35221 agttgcttca gtagtattga tgctgatacg gctgtttttt aagcatagac ggtcatttga
    35281 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
    35341 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
    35401 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
    35461 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
    35521 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
    35581 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
    35641 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
    35701 aaagcagaag aaacaagtaa tcgcgtaatt aacggagttg tcgaactgcc gaaagatcag
    35761 gccgttctga ttgaaccgtt tgacacggtc accgtccagg gcttttatcg tagccagcca
    35821 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc ttactacatc
    35881 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
    35941 aaagatgcga agaaacgtat cgtccagagc ccggacgtaa tcccggcaga ttccgaagca
    36001 ggacgtgcag ctctggctgc cggtggcgaa gctgcgaaga aagttgagat cccgggtgtt
    36061 gcgactaccg catcaccaag ctctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
    36121 ggcgggcgtt acactgtcac gcttcctgac ggcactaaag tcgaagaact gaacaaagcg
    36181 accgcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
    36241 actgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
    36301 atcacccgcc agtggcagga acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
    36361 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
    36421 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
    36481 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
    36541 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
    36601 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
    36661 cactgctcat cggctggggt attactggcg caggcatttt attgctggcc tttgccatgc
    36721 tgatcctcac gcgcattcgt cccgaacttg acggcggtat ctttacctat gcccgcgaag
    36781 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
    36841 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
    36901 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat cgtcggtgca tcggctttat
    36961 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
    37021 tggcaacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
    37081 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cccgtttggg
    37141 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
    37201 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
    37261 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggc gtggtggcac
    37321 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
    37381 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
    37441 taagctggac catcatggcg gcagaagttc cgttcctggc agccactcat aaagcattcc
    37501 cgcgcatttt tgcgcgccag aacgcacaag ctgcgccatc cgcttcactg tggctgacca
    37561 atatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacat
    37621 tgctgaccat cgcctcagag atgattctgg tgccctattt cctggtcggc gcgttcttgc
    37681 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
    37741 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcgc
    37801 cggggctgct ggttttcctg tatgcgcgta aaacgcatac acatgataat gtactgaacc
    37861 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
    37921 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
    37981 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgca ctcgcctggc
    38041 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacgcactat ccagccattg
    38101 atggactaat taaggcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    38161 tgatggcgtt tgccgatgaa gtactaaaaa gtacccatgg tctgcgtgct attttgcata
    38221 acgccagcgc gtggatggcg gaaaaaccgg gtgcgccact gtccgacgta ctggcttgca
    38281 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctagaa agattactgc
    38341 gtgggcacgg acacgcctcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    38401 gtagcgacaa acatattgcc tatgctgcaa gcaaagcggc actggataat atgactcgct
    38461 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    38521 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    38581 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagctgct
    38641 ttgtcaccgg acgcagtttc cctcttgatg gcggtcgtca tctgcgttaa tgcagcttta
    38701 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    38761 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga cgccaggtaa acaaaatagg
    38821 gaataatcga ccacatgcta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    38881 tggcaacaat ataatgcgcg atgagcgcaa aggtcgggaa aagtgggatc agcccggcga
    38941 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    39001 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    39061 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    39121 tgtgacgttt ttatatctac cgtgaataat atgaacacta tcgtatttgt ggaagatgat
    39181 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    39241 gagccgcgcg gcgaccaggc cgaagaaact attctgcgag aaaatccgga tttggtgtta
    39301 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    39361 tggtccggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    39421 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgttagcg
    39481 cgtttacgtt tgcatttgcg tcagaatgag caagccacgc tgaccaaagg tcttcaggaa
    39541 acgtcactga ctccctacaa agccctgcat ttcggcacgt tgaccatcga tcctatcaac
    39601 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    39661 tgggaattag ctacccatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    39721 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    39781 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    39841 tatctttttg cgcctcatgc atgggaataa gcgatgaaaa aactgtttat ccagttttac
    39901 ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt gtacaaattt
    39961 accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc gctgtatctg
    40021 atgcgcagcg aattgcgtga gatcccccca cacgattggg gtaagacgct gaaggagatg
    40081 gatttaaatc tctctttcga tctgcgtgtc gagccgctga gtaaatatca tcttgatgat
    40141 atttccatgc accggctgcg tggcggcgaa attgtcgccc tggacgatca gtacacgttt
    40201 ttgcaacgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc ttatctttat
    40261 tacctccatc agatgcgatt actggatatc gccctgatcg cttttattgc catttccctc
    40321 gcctttccgg tgtttatctg gatgcgtccg cactggcagg atatgttaaa actggaagca
    40381 gcggcgcaac gatttggcga tgggcatctc aatgagcgta tccactttga tgagggttcg
    40441 agctttgaac gacttggcgt cgcatttaac cagatggcgg acaatatcaa cgccttgatt
    40501 gccagcaaaa aacagcttat tgacggtatc gctcatgaac tgcgaacacc gttagtgcgc
    40561 ctgcgttatc gactggagat gagcgataac ctgagcgccg ccgaatccca ggcgttgaat
    40621 cgcgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc ccgactcgat
    40681 cgcccacaaa acgagcttca tcttagcgaa ccagacctgc cgttgtggct gtcaacgcat
    40741 ctggcagaca ttcaggcagt aacgcccgat aaaacggtac ggattaaaac gctcgcgcaa
    40801 ggccattatg cggcgttgga tatgcgctta atggagcgcg tgctggataa tttgctcaat
    40861 aacgccctgc gctactgtca ctcaacggtt gaaaccagcc tgctactgtc ggggaataga
    40921 gcgacattaa ttgttgagga tgatggccca gggattgccc cagaaaaccg cgaacatatc
    40981 tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg ctgcgggctg
    41041 gggttggcga ttgtccactc tatagcacta gcaatgggcg gtacggttaa ttgtgacacc
    41101 agcgaactgg gtggtgcccg cttctcgttt agttggccgt tatggcataa catcccgcaa
    41161 tttacctctg cttgacatta cgcgcacgat ggtcaagtca ccaccattgt gctataaatt
    41221 aaggatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt agaccgactc
    41281 aacactacct ttcgccagat ggaacaagag ctggctgcat ttgccgctca tcttgagcaa
    41341 cacaagctat tggttgcccg cgtgttctct ttgccggagg taaaaaaaga ggatgagcat
    41401 aatccgctta atcgtattga ggtaaagcaa catctcggca acgacgcgca gtcgctggcg
    41461 ttgcgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag cagcaaggcc
    41521 gctgttcgtc tacctggcgt gttgtgttac caggtcgata acctttcgca agcagcgttg
    41581 gtcagtcata ttcagcacat caatcaactc aagactacgt tcgagcatat cgtcacggtt
    41641 gagtcagaac tccccacagc ggcgcgtttt gaatgggtgc atcgtcattt gccggggctg
    41701 atcaccctta atgcttaccg aacgctcact gttctgcacg accccgccac tttacgtttt
    41761 gggtgggcca ataaacatat cattaagaat ttgcatcgtg atgaagtcct ggcacagctg
    41821 gaaaaaagcc tgaaatcacc gcgcagtgtc gcaccgtgga cgcgcgagga gtggcaaaga
    41881 aaactggaga gagagtatca ggatattgct accctgccac aggacgcgaa gttaaaaatc
    41941 aaacgtccgg tgaaggtgca gccgattgcc cgcgtctggt acaagggaga tcaaaaacaa
    42001 gtacaacacg cttgccctac accgcttatt gcgctgatta atcgggataa tggcgcgggc
    42061 gtgccggacg ttggggagtt gttaaattac gatgccgaca atgtgcaaca ccgttataaa
    42121 ccacaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt tgcagattaa
    42181 cgcccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc aaactcggct
    42241 tcgctaagat accccagcgc aagggctgca gcttttaagg tcagcccttc tttatgcgct
    42301 tttttggcga tctcggcggc tttgtcataa cctatgtggg tgttaagcgc agtcaccagc
    42361 atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat acccactgcg
    42421 cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg caggaaattg
    42481 tggatcacca tcggacggaa aacgttcagt tcaaagttac cggaagcgcc acccatgttg
    42541 atcgccacgt cgttccccat cacctgacag caaagcatgg ttaatgcctc acactgtgtt
    42601 gggttcactt ttcccggcat gattgagctg cccggctcat tttccgggat tgagatttca
    42661 ccaattccac agcgcgggcc agaggccagc cagcggacat cattggcaat tttcatcagt
    42721 gacgcagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca ggtcgccagc
    42781 gcttcaaatt tgttcggcgc ggtaacaaac ggtgcgcagg taatgactgc cagttcatct
    42841 gccacgcgac gcgcatactc cggatgggta tttagtccag tacccacagc tgtaccgccc
    42901 atagccagtt ccgctacgtg aggcaggctg tattcaatat gtttgagatt atgctcgagc
    42961 atcgctaccc agccggaaat ctcctgcccc agtgttagcg gcgtggcgtc ctgcaagtgg
    43021 gttcgaccga tttttacgat atcggcaaat gcacgcgatt tttcactcag cgtctgtgtc
    43081 agggttttaa gctgcggaat gagttgcttg cgcagcgcca gcagcgccgc aacgtgcatc
    43141 gccgttggaa agacatcgtt agaactttgg cttttgttca cgtcgtcgtt agggtgaact
    43201 ttacgctcca tcccacgcac gccgccgagt aattcactgg cccggttagc cagcacttcg
    43261 ttcatgttca tgttactttg cgtgccggag ccagtctgcc agatagccaa tgggaattcg
    43321 tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct cgctttctct
    43381 tcagacaaca agcctaaatc ttcattaact tttgccgctg cgcgcttggt tagcgccagc
    43441 gcatgaatca gtgaggtggg cattttctcc gtcgaaatgc ggaaatgctc cagcgagcgt
    43501 tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgcccccat cgaatctttt
    43561 tcgctgcgta ctgtattcat ggcctgctcc tcacctgatt aattttttct ttctgttttg
    43621 ctttcgttaa gcaactttta gctcactcat tatttactat ttgataacaa atgtttggtc
    43681 tttcgtgcca tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgggt
    43741 gcactgtgtg agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa
    43801 cgccgggaaa tcttccactt caattttcca gatggcttcc attcccagtt ccggatattc
    43861 aacacattcc aggctcttaa tactaccctg cgccaataca gcggccggac caccgatact
    43921 gccaaggtag aagccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc
    43981 tttcgccagc atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat
    44041 ccggccggcg gtcgttgggc caagagaacc ggaagcataa ccttccggcg ttttggccgg
    44101 acccgcgtag taaatcggat gatctttaat gtactgcggc agcccttcac cgttatccat
    44161 ccgctctttc agtttggcgt gagcaatatc acgaccaacg ataatcgtgc cattcagcga
    44221 taagcgtgta gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt
    44281 aaggtcaacg cgcaccgctt cgccttctcc cgctttgcgc agctcttccg ggatatattt
    44341 gcctggatta tgttccagtt tttcgatcca aatcccctga cggttgatct ttgctttgat
    44401 attacggtca gcagaacaag agacgcccat accgaccggg caggatgcgc cgtgacgtgg
    44461 caggcgaatc acgcggatgt cgtgagcgaa gtatttaccg ccaaactgcg cacccagacc
    44521 aagattttgc gcttcgatca gcaattcttt ttccagttcc acatcgcgga acgcctgtcc
    44581 gtgctcattc ccttccgttg gcagttcatc atagtattta gcggaagcca gtttcaccgt
    44641 tttaaggttc gtttctgcag aagttccacc aataacgaac gcaatatgat acggaggaca
    44701 ggccgccgta cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt
    44761 tagtaacgct ttggtttcct gatagagata cgtcttgttt gccgaaccgc cacctttggc
    44821 gatacagagg aatttgtact cgtcgccatc aacggcataa agatcgatct gcgctggtag
    44881 attggtgccg gtattcacct ctttatacat atccagcggc gcgttttgcg agtagcgcag
    44941 attatcttcg atataagtgt tatagacacc gcgtgccagc gccgcttcat caccgccacc
    45001 ggtccataca cgctgccctt ttttaccaac aataatcgcg gtgccggtat cctgacaggt
    45061 tggcagaacg cctttcgccg cgatgtcgga gttgcgcagg aattgcagcg ccacatattt
    45121 atcattttcg ctggcttccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg
    45181 acgcagcata aatgacgcat catgaaacgc ctgacgcgcc aacaaagtta acgcttcggg
    45241 tgcgactttc aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cgctggttag
    45301 caggtaatac tcagtatcat cttttttgag tggaaaagga gcctgataat gaaagggttt
    45361 gtttgacatt gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt
    45421 tgccctgtta aaagcgagta acaatatcct acacactttt ttaacaaaaa ctgagactag
    45481 tacgactttt tgcggctcca ggttacttcc cgtaggattc ttgctttaat agcgggatta
    45541 atttccacat ataaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat
    45601 gcctggggca gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag
    45661 ccgatggccg agctgtggat gggcgcacat ccaaaaagca gttcacgagt gcagaatgcc
    45721 gccggagata tcgtttcact gcgtgatgtg attgagagta ataaatcgac tctgctcgga
    45781 gaggccgttg ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca
    45841 cagccgctct ccattcaggt tcatccaaac aaacgcaatt ctgaaatcgg ttttgccaaa
    45901 gaaaatgccg caggtattcc gatggatgcc gccgagcgta actataaaga tcctaaccac
    45961 aagccggagc tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgaattt
    46021 tccgagattg tctccctact ccagccagtc gcaggtgcac atccggcgat tgctcacttt
    46081 ttacaacagc ctgatgccga acgtttaagc gaactgttcg ccagcctgct gaatatgcag
    46141 ggggaagaaa aatcccgcgc gctggcgatt ttaaaatcgg cccttgacag tcagcagggt
    46201 gaaccgtggc aaacgattcg tttaatttct gaattttacc cggatgacag cggtctgttc
    46261 tccccgctgt tgctgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct
    46321 gaaacgccgc acgcttacct gcagggcgtg gcgctggaag tgatggcgaa ctccgataac
    46381 gtgctgcgtg cgggtctgac gcctaaatac attgatattc cggaactggt tgccaatgtg
    46441 aagttcgaag ccaaaccggc taaccagttg ttgacccagc cggtgaaaca aggtgcagaa
    46501 ctggacttcc cgattcctgt ggatgatttt gccttctcgc tgcatgacct tagtgataaa
    46561 gaaaccacca tcagccagca gagtgccgcc attttgttct gcgtcgaagg cgatgcaacg
    46621 ctgtggaaag gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc
    46681 aacgaatcac cggtgactgt caaaggccac ggccgtttag cgcgtgttta caacaagctg
    46741 taagagctta ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct
    46801 tcaggcggcc agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt
    46861 agcggtaggc gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg
    46921 caagaagatt gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact
    46981 gaccgctcct gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg gcgtattcag
    47041 cagtcagctg caactgttgg tgaaacccat tgccgggaaa gaaaatccgt ggattaaaag
    47101 cggtcagagc gtcatcttca acgaatcggt tgatcatggt cccttccccc ttgcccagct
    47161 taaaaaactg aacctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataacga
    47221 agttagcaag ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcccg
    47281 cattggttac agcggagatt ccagttccga tatttcgctc aagccactga attacgagca
    47341 aaaggatgaa aaagtcgcct ttagcggcgg cgagttccag ttaaatgcag acagagacgg
    47401 caaagctatc tccctttccg gggaggcgca aagtggtcgg atagacgcgg ttaacgaata
    47461 taaccagaaa gtacagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc
    47521 aagttttggt gagcgcgtag gaaatcaaaa actgtcactg gaaaaaatga ccatttcagt
    47581 ggaaggcaaa gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt
    47641 caatgacggt aaaacgatca atagccaact ggattactcg ctaagcagcc tgaaggtaca
    47701 gaatcaggat ctgggcagcg gcaagctgac tttaaaagtc ggccagattg atggtgaagc
    47761 ctggcatcag tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga
    47821 aattgccaac aatcccgaac tttatcagga gaaagtgacg gaagccttct ttagcgccct
    47881 gccgctgatg ttgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag
    47941 tcagggtgaa agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga
    48001 agcgccgcaa acgctggcgc aggaagtaga tcgttcggtt aaatctctgg atgcgaaact
    48061 gaccattccg gtggatatgg caactgagtt tatgactcaa gtagcgaagc tggaaggtta
    48121 tcaggaagat caagcaaaaa aactggcgaa acagcaagtt gaaggtgcgt cagcaatggg
    48181 gcagatgttc cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatgc
    48241 taacggtcag atcacgttaa acgggcagaa aatgccactg gaagatttcg ttggtatgtt
    48301 tgcaatgccg gcattaaacg ttccggtcgt acccgctctt ccgcagcagt aattcacagc
    48361 caaaacgccg gaggtttttc tctccggcgt tatttcttac ttcagcataa agtcatactg
    48421 aatactcatt ttcacatccc agtaattcag gcgatcgccc agcgcactgc catcgcgctt
    48481 ttcccgccct ttatcggcag caagaaagaa attcatccct tgtaacggcc cttcgctgac
    48541 ccgatagttc ataccaccta agatacccgc gccttcaatc cgcacatctc gtttactgga
    48601 ttcatagccg cgagtcacaa tcggtgcaaa tgtcagcgta aattgcggcg ttaaacgata
    48661 gttgacaccc aattgccagg actgcattcc ttcacggtta cgatcaagac ttaatgaaaa
    48721 gggatagcca atgtcggtat ccaccacggc accattcatt gcatggcgca aataaccacc
    48781 actgccaaat acgcttcccc acggcgcatt ccaggtcaat tgtccactaa ccaacgccgt
    48841 ttcattgggc tggctggtat tacgacttaa accttcgagc tgcgcgtaaa agccgagcaa
    48901 ctcccctttc aacgtataat tcgggtcaag cggtatgctt ttctcgagaa tactttgtat
    48961 ctgattggat acatctttct gtaccgatgc ccccagagaa tagcgactgg tatcatcatg
    49021 ccaactggcg gctaaaaaag aacgtgcgcg gtgtttatcg tattttcccg tctggctgtt
    49081 atacacccct tcatcacgaa taccggttgt tgaatcggtc cataaaaata ctcgtgaacg
    49141 ccacattccc cacagatcaa tatttggcgt taaagcccaa taacctaatg ctccttcaga
    49201 aactgtcggt aatgtacgtt gcgaactgcc agccattaac ggaatacgat aaggtaagct
    49261 accataacta taatccgtcc cgaagcgacc taattttagc aggctatttt cactgggttt
    49321 tatttttact gcccccaggg caaagccaaa acttttatca ccatcaagat accaccgggt
    49381 actcatatca gcacgagcac ctaatttata aacataatac gccccgcctt caacgccaat
    49441 aatattggaa taataaccac tattgaaatc gagcaatcct ccctgtaccc atgcgtaaat
    49501 atctcttccc gcgccagcac tgggcttatc agccctgcgc aattcgtttt tcagtctgat
    49561 gcgtagtccc gcttcatcgt cagccagttg cgccgcgtaa gcagaggtaa ggccagaggc
    49621 agccgtcagg caaataacgg ccatggccac tatttttctc atagt
//
LOCUS       DAYPHN010000013        49586 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000013 DAYPHN010000000
VERSION     DAYPHN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.013"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACG0BW_000796"
     CDS             complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACG0BW_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFK7257793.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLKVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1572..2795)
                     /gene="hemX"
                     /locus_tag="ACG0BW_000797"
     CDS             complement(1572..2795)
                     /gene="hemX"
                     /locus_tag="ACG0BW_000797"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFK7257794.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQADTPAAAPQGE"
     gene            complement(2817..3557)
                     /gene="hemD"
                     /locus_tag="ACG0BW_000798"
     CDS             complement(2817..3557)
                     /gene="hemD"
                     /locus_tag="ACG0BW_000798"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFK7257795.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGERDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3554..4495)
                     /gene="hemC"
                     /locus_tag="ACG0BW_000799"
     CDS             complement(3554..4495)
                     /gene="hemC"
                     /locus_tag="ACG0BW_000799"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFK7257796.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4882..7428
                     /gene="cyaA"
                     /locus_tag="ACG0BW_000800"
     CDS             4882..7428
                     /gene="cyaA"
                     /locus_tag="ACG0BW_000800"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFK7257797.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7468..7788)
                     /gene="cyaY"
                     /locus_tag="ACG0BW_000801"
     CDS             complement(7468..7788)
                     /gene="cyaY"
                     /locus_tag="ACG0BW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFK7257798.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7911..8065)
                     /locus_tag="ACG0BW_000802"
                     /pseudo
     CDS             complement(7911..8065)
                     /locus_tag="ACG0BW_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8254..8457
                     /gene="yifL"
                     /locus_tag="ACG0BW_000803"
     CDS             8254..8457
                     /gene="yifL"
                     /locus_tag="ACG0BW_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFK7257799.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8494..9318
                     /gene="dapF"
                     /locus_tag="ACG0BW_000804"
     CDS             8494..9318
                     /gene="dapF"
                     /locus_tag="ACG0BW_000804"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFK7257800.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9315..10022
                     /gene="yigA"
                     /locus_tag="ACG0BW_000805"
     CDS             9315..10022
                     /gene="yigA"
                     /locus_tag="ACG0BW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFK7257801.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHIHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10019..10915
                     /gene="xerC"
                     /locus_tag="ACG0BW_000806"
     CDS             10019..10915
                     /gene="xerC"
                     /locus_tag="ACG0BW_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFK7257802.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10915..11631
                     /gene="yigB"
                     /locus_tag="ACG0BW_000807"
     CDS             10915..11631
                     /gene="yigB"
                     /locus_tag="ACG0BW_000807"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFK7257803.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11715..13877
                     /gene="uvrD"
                     /locus_tag="ACG0BW_000808"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11715..13877
                     /gene="uvrD"
                     /locus_tag="ACG0BW_000808"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFK7257804.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPYILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDKGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13922..14824)
                     /locus_tag="ACG0BW_000809"
                     /pseudo
     CDS             complement(13922..14824)
                     /locus_tag="ACG0BW_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
     gene            complement(14907..15671)
                     /gene="yigE"
                     /locus_tag="ACG0BW_000810"
     CDS             complement(14907..15671)
                     /gene="yigE"
                     /locus_tag="ACG0BW_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFK7257805.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLVLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHIYMKGGAIPWQRYPFVTMISVEWKG"
     gene            15833..15889
                     /gene="ysgD"
                     /locus_tag="ACG0BW_000811"
     CDS             15833..15889
                     /gene="ysgD"
                     /locus_tag="ACG0BW_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFK7257806.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16041..16991
                     /gene="corA"
                     /locus_tag="ACG0BW_000812"
     CDS             16041..16991
                     /gene="corA"
                     /locus_tag="ACG0BW_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFK7257807.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17034..17414)
                     /gene="yigF"
                     /locus_tag="ACG0BW_000813"
     CDS             complement(17034..17414)
                     /gene="yigF"
                     /locus_tag="ACG0BW_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFK7257808.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKTL
                     KVRQRLTIKMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(17428..17808)
                     /gene="yigG"
                     /locus_tag="ACG0BW_000814"
     CDS             complement(17428..17808)
                     /gene="yigG"
                     /locus_tag="ACG0BW_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFK7257809.1"
                     /translation="MLRIFIPTSNGKISRRRYIFSFILINFIFAFLIIFFNDGDAGFL
                     VIVSTIALHYLVININCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEHFDCSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(17903..18793)
                     /gene="rarD"
                     /locus_tag="ACG0BW_000815"
     CDS             complement(17903..18793)
                     /gene="rarD"
                     /locus_tag="ACG0BW_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFK7257810.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSATSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(18845..19312)
                     /gene="yigI"
                     /locus_tag="ACG0BW_000816"
     CDS             complement(18845..19312)
                     /gene="yigI"
                     /locus_tag="ACG0BW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFK7257811.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            19477..20346
                     /gene="pldA"
                     /locus_tag="ACG0BW_000817"
     CDS             19477..20346
                     /gene="pldA"
                     /locus_tag="ACG0BW_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFK7257812.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            20479..22308
                     /gene="recQ"
                     /locus_tag="ACG0BW_000818"
     CDS             20479..22308
                     /gene="recQ"
                     /locus_tag="ACG0BW_000818"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFK7257813.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRSESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            22372..22992
                     /gene="rhtC"
                     /locus_tag="ACG0BW_000819"
     CDS             22372..22992
                     /gene="rhtC"
                     /locus_tag="ACG0BW_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFK7257814.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(23054..23674)
                     /gene="rhtB"
                     /locus_tag="ACG0BW_000820"
     CDS             complement(23054..23674)
                     /gene="rhtB"
                     /locus_tag="ACG0BW_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFK7257815.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            23785..24807
                     /gene="pldB"
                     /locus_tag="ACG0BW_000821"
     CDS             23785..24807
                     /gene="pldB"
                     /locus_tag="ACG0BW_000821"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFK7257816.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRFILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            24815..25615
                     /gene="yigL"
                     /locus_tag="ACG0BW_000822"
     CDS             24815..25615
                     /gene="yigL"
                     /locus_tag="ACG0BW_000822"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFK7257817.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            25691..26590
                     /gene="bioP"
                     /locus_tag="ACG0BW_000823"
     CDS             25691..26590
                     /gene="bioP"
                     /locus_tag="ACG0BW_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFK7257818.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSQRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(26478..27431)
                     /gene="metR"
                     /locus_tag="ACG0BW_000824"
     CDS             complement(26478..27431)
                     /gene="metR"
                     /locus_tag="ACG0BW_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFK7257819.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGISPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            27667..29928
                     /gene="metE"
                     /locus_tag="ACG0BW_000825"
     CDS             27667..29928
                     /gene="metE"
                     /locus_tag="ACG0BW_000825"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFK7257820.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEAQALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELSLLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(29967..30782)
                     /gene="ysgA"
                     /locus_tag="ACG0BW_000826"
     CDS             complement(29967..30782)
                     /gene="ysgA"
                     /locus_tag="ACG0BW_000826"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFK7257821.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKRYGGKKSL"
     gene            31044..31805
                     /gene="udp"
                     /locus_tag="ACG0BW_000827"
     CDS             31044..31805
                     /gene="udp"
                     /locus_tag="ACG0BW_000827"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFK7257822.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            31946..33373
                     /gene="rmuC"
                     /locus_tag="ACG0BW_000828"
     CDS             31946..33373
                     /gene="rmuC"
                     /locus_tag="ACG0BW_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFK7257823.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            33468..34223
                     /gene="ubiE"
                     /locus_tag="ACG0BW_000829"
     CDS             33468..34223
                     /gene="ubiE"
                     /locus_tag="ACG0BW_000829"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFK7257824.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            34237..34842
                     /gene="ubiJ"
                     /locus_tag="ACG0BW_000830"
     CDS             34237..34842
                     /gene="ubiJ"
                     /locus_tag="ACG0BW_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFK7257825.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            34839..36479
                     /gene="ubiB"
                     /locus_tag="ACG0BW_000831"
     CDS             34839..36479
                     /gene="ubiB"
                     /locus_tag="ACG0BW_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFK7257826.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            36558..36827
                     /gene="tatA"
                     /locus_tag="ACG0BW_000832"
     CDS             36558..36827
                     /gene="tatA"
                     /locus_tag="ACG0BW_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFK7257827.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            36831..37346
                     /gene="tatB"
                     /locus_tag="ACG0BW_000833"
     CDS             36831..37346
                     /gene="tatB"
                     /locus_tag="ACG0BW_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFK7257828.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            37349..38125
                     /gene="tatC"
                     /locus_tag="ACG0BW_000834"
     CDS             37349..38125
                     /gene="tatC"
                     /locus_tag="ACG0BW_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFK7257829.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTKE"
     gene            38167..38949
                     /gene="tatD"
                     /locus_tag="ACG0BW_000835"
     CDS             38167..38949
                     /gene="tatD"
                     /locus_tag="ACG0BW_000835"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFK7257830.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLAHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(38946..39434)
                     /gene="rfaH"
                     /locus_tag="ACG0BW_000836"
     CDS             complement(38946..39434)
                     /gene="rfaH"
                     /locus_tag="ACG0BW_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFK7257831.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            39601..41094
                     /gene="ubiD"
                     /locus_tag="ACG0BW_000837"
     CDS             39601..41094
                     /gene="ubiD"
                     /locus_tag="ACG0BW_000837"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFK7257832.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            41140..41841
                     /gene="fre"
                     /locus_tag="ACG0BW_000838"
     CDS             41140..41841
                     /gene="fre"
                     /locus_tag="ACG0BW_000838"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFK7257833.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(42032..43195)
                     /gene="fadA"
                     /locus_tag="ACG0BW_000839"
     CDS             complement(42032..43195)
                     /gene="fadA"
                     /locus_tag="ACG0BW_000839"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFK7257834.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(43205..45394)
                     /gene="fadB"
                     /locus_tag="ACG0BW_000840"
     CDS             complement(43205..45394)
                     /gene="fadB"
                     /locus_tag="ACG0BW_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFK7257835.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            45584..46915
                     /gene="pepQ"
                     /locus_tag="ACG0BW_000841"
     CDS             45584..46915
                     /gene="pepQ"
                     /locus_tag="ACG0BW_000841"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFK7257836.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            46915..47529
                     /gene="yigZ"
                     /locus_tag="ACG0BW_000842"
     CDS             46915..47529
                     /gene="yigZ"
                     /locus_tag="ACG0BW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFK7257837.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            47568..49019
                     /gene="trkH"
                     /locus_tag="ACG0BW_000843"
     CDS             47568..49019
                     /gene="trkH"
                     /locus_tag="ACG0BW_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFK7257838.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSILWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            49031..49576
                     /gene="hemG"
                     /locus_tag="ACG0BW_000844"
     CDS             49031..49576
                     /gene="hemG"
                     /locus_tag="ACG0BW_000844"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFK7257839.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        11548 a        12705 c        13367 g        11966 t
ORIGIN      
        1 cgcaggagaa atattcactc aggaagttat tactcaggaa gcaaagagga ttacagaatt
       61 atctcataac aagtgttaag ggatgttatt tcccggttct ctgtggcata ataaacgagt
      121 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      181 agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat cagacaccat
      241 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      301 ccgtaataag agcaggcgtt tttttatgta tcagaaaggt cccggaggtg cttgcctccg
      361 ggtgagaaag aactactgtg gcgggttgtt ctgcaacgtt aacatcaaac cgtcgcggcg
      421 catcgctgcg gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      481 atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag gcgagcgatg cttcctgcca
      541 ttcgccgtgc ttcatcagcg actgacccag tgtgctccac aacaacgggc gatcgcccac
      601 gtttttgatc tgttggcgca gcactttttc cagctgttcc gggttattgg ttttcagacg
      661 cgggatcggc agcagcaggc gatcgtcata ctggcgtttc aggccgtcga tgataatttg
      721 ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca cctgcaacgc
      781 cacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcgctacc
      841 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
      901 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagcgaact
      961 ccatgcacct gtgcggatat acgcctgttc cgccagacgt aatacttccg gatggcgtgg
     1021 cgtaactttc agcagcttat ccacgccgtg gcgtgcagca tggttttcat tacgggccag
     1081 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctccgctgc
     1141 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggcagc
     1201 cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca tcagcttttc
     1261 aacttgctga taatcgcctt ccgctagttt cagcagcgcc tgttcggtct gcttacgggc
     1321 acggcgacgc ttacgtccga caaaccaccc acgggtgtgt gcgcctgtgc ggaagatccg
     1381 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa taatcgccag
     1441 gccagtgacg ctggtttcaa tattgtagtt gtcggtctga atcaatacgt aaccctgatg
     1501 accggcaatc atcgggccaa ccactatccc cgcaatcagc aaaacaaaga gcaataacac
     1561 ttttagcatg gttattctcc ttgcggcgcg gctgccggag tatcagcttg cggtgcaggt
     1621 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggttt agcttccgtt
     1681 gtccccgctg ccggttgtgc caacaggtta cgcacgcgag tctgcatcag tttttccagc
     1741 atcgcctggc tttgcagggt ttccgggaga tccatcgaga tattttgctg gcttaactgg
     1801 tccacctcgt cgaggaacgc tttggtggtg gcatcatcag tatcgtagta agcacgtacc
     1861 caggtggaga cgttctccag cgcctggcga taagtctctt cctgatgacg cggtacggct
     1921 tgtgccgcga ccagcaggcg agagcgaata ttttcgcgca gatagatatc ctgatttggc
     1981 gctaacagcg gtacggcggt gtcatcacga cggcgaatcg taatgaagtt gtccataaag
     2041 ttctgccagc ttttttgtag attgatacgc cattcgctga tggaactgga aagctcttca
     2101 ccgtcggaat ccatcggcga accatcgctg tcattatcgg ccagacgcag gttatctacc
     2161 tgatttgaaa gctgattaag cttaaggatg atgccgtcat aatccacctg cgataccgca
     2221 gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg taatcagact cgggtcattc
     2281 atatccgcca ggctggcatc tgcacttttc agcaacgctg cagcggtcgt gacgtcttga
     2341 tcgctccaca gcttccgtcc tgcgagtttc accagaaaat cggcctgagc cagcagccag
     2401 gttttagcat cgctgccgga aatggtggcg accttttgtt ggacttcatc caactgtttt
     2461 gccagcgttt cttgctgacg attcgcctgt tcaagctgcg tagcctgttg cttgataatg
     2521 ccttccagct cggctttttg gctctcctgg gctttttgca atgccgtcag ttggttagcc
     2581 agggcatcgc tggtggcggt ctgattgacg gcctgttgtt taccccagcc atacaaaccg
     2641 acgcccgccg ccagagcaat agcaatagcc accgcgctga gaatcaatgc ggtattgttc
     2701 ttactctttt tttctgttgc gacaggttgt gacgtggtgt ccacggcctc cctggtctct
     2761 tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg cttcctgtta tgagagttat
     2821 tgtaatgccc gtaaaagcgc atcgttgtca gcgttatcgg cgaccttaat gtcttgccag
     2881 cccagttccc gggcgagttt cgccaaacgc tcactgacga ccaatagtcg acagtgtagt
     2941 aaccagtgct cacgatacca ttgtgggatc agcgaccaga gttgctgcaa catttcaccg
     3001 ctggtaacaa cgaccgtcgt cacctcgcgg gattgccagc gcatcgcttc ttctgcacca
     3061 tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag tgacctcagc accgcgcgcc
     3121 gtcagggtat ccccaattag ctcacgaccg ccattgccac gtaatatcag cgcacgtttg
     3181 cccgcaatat tttgtaattc aggtaattgt agcaagactt cgctgatttc ccgatcctgc
     3241 gggtagagaa tcttctgtcc gcttacggta tgtagcgcca gtgcggtggt gcgtccaatg
     3301 gcgaaataat caggtagtcg gggccattta cgatcttgct gatgcagctg tgattgggca
     3361 aaagcaaccg cgtgttgcga gagggcaaac aacagatccc tctcccccag cgctgcaagt
     3421 tgatcagcaa gttgcggtaa ttgtcgaccc ggagaaaact caatcagcgg aaaatgccag
     3481 gccacctgcc ccagtgtgcg cagacggctc actaactctt ctccagcggg agacgggcgg
     3541 gtgaccagga tactcatgcc ggggcgtctc cgttatagac ttcagcaagg atctcgcgcg
     3601 cgccgttatt cagtagctct tctgccagcg aaatccccat ttgttcggca tcttgcggcg
     3661 caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg cgcgccgacc agcgcacgca
     3721 gccagatttc gccatcaata agctcggcgt agctaccaat tggcacctga catccgcctt
     3781 cgagacgggt attcatggcg cgttctgcgg taacgcgtag tgcagtttcg tggtgattca
     3841 gcgcggcaag cagctcgcga gtgcgtgtat catcaaggcg gcattcaata ccgaccgcac
     3901 cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc ggcgcgaata cgtgactcca
     3961 gacctaaacg ttttagtccg gctacggcaa gaatgatggc atcgtattcg ccgttatcca
     4021 gtttgctcag gcgagtgccg acgttgccgc gcagggagcg gataatcaga tccgggcggc
     4081 gttcagccag ttggcactgg cgacgtaaac tggacgtccc gacgatactg cctgccggta
     4141 acgcatccag actgtcatag ttattggaca caaaggcatc gcgaggatct tcacgctcac
     4201 aaatagtgac cagtcccaga ccttgcggga attcaaccgg cacatctttc attgaatgta
     4261 cggcgatatc ggcgcgattt tcgaggagcg cgacttccag ctcttttaca aataagcctt
     4321 ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc gccgcgcgtc accatcggta
     4381 ccagttcaac gaccaggccc ggatggctcg ccatcaactt gtctttgaca tagtgtgcct
     4441 gccagagtgc aagtgggctt tggcgtgtgg caattcttaa aacattgtct aacatgcttg
     4501 ttaccgtcat tatcatccgt ggtccatcct aacatccttg ccagagcgat gtcagtgttg
     4561 tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt ctggcagtgg atcctgacag
     4621 gcatttcacg ccgtcgtaat aaggaattta cagagaataa acggtgctac acttgtatgt
     4681 agcgcatctt tctttacggt caatcagcaa ggtgttaaat tgatcacgtt ttagaccatt
     4741 ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg taacggttac ctttgacata
     4801 cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc ggaatcacag tcatgacggg
     4861 tagcaaatca ggcgatacgt cttgtacctc tatattgaga ctctgaaaca gagactggat
     4921 gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta tggggcctgc attccaacag
     4981 gtctacagtc tactgccgac attgttgcac tatcaccatc cgctaatgcc gggttacctt
     5041 gatggtaacg ttcccaaagg catttgcctt tacacgcctg atgaaactca acgccactac
     5101 ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg atccgccgaa aggtgagctt
     5161 ccaattactg gtgtatacac catgggcagc acctcgtccg tagggcaaag ttgttcctct
     5221 gacctggata tctgggtctg tcatcaatcc tggcttgata gcgaagagcg ccagttacta
     5281 caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc tgggcgtgga agttagcttc
     5341 ttcctgattg atgaaaatcg cttccgtcat aatgaaagcg gcagcctggg gggcgaagat
     5401 tgtggctcca cccagcatat actgctgctt gacgaatttt accgtaccgc cgtgcgtctc
     5461 gctggtaagc gtattctgtg gaatatggtg ccgtgcgacg aagaagagca ttacgacgac
     5521 tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa atgaatggct ggatctcggt
     5581 ggtttaagct cactttctgc tgaagagtac tttggtgcca gcctttggca gctctacaag
     5641 agtattgatt ccccatacaa agcggtgctg aaaacactgc tgctggaagc ctattcctgg
     5701 gaatacccga acccacgtct gctggcgaaa gatatcaaac agcgtttgca cgacggcgag
     5761 attgtatcgt ttggcctcga cccgtactgc atgatgctgg agcgtgttac tgagtacctg
     5821 acggcgattg aagatttcac ccgtctggat ttagtgcgtc gctgcttcta tttaaaagtg
     5881 tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc gtcgtgaagt gctaacccag
     5941 ttagtgaaag agtgggaatg ggacgatgcg cgtctggcaa tgctcgataa ccgcgctaac
     6001 tggaagattg atcaggtgcg tgaggcgcac aacgagctgc tcgatgcaat gatgcagagc
     6061 taccgtaatc tgatccgctt tgcgcgtcgc aataacctga gcgtctccgc cagtccgcag
     6121 gatatcggcg tgctgacgcg taaactgtac gccgcgtttg aagcattgcc aggtaaagtg
     6181 acgctggtaa acccgcagat ttcgcccgat ctctcggaac cgaatctgac ctttatttat
     6241 gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt ataaccgcgc gccaaatatt
     6301 gagtcgatca tcagccatca gccgctggaa tataaccgtt acctgaataa actggtggcg
     6361 tgggcatggt ttaacggcct gctgacctcg cgtacccgct tgtatattaa aggcaacggc
     6421 attgtcgatt tgcctaagtt gcaggagatg gtcgccgatg tgtcacacca tttcccgctg
     6481 cgcctgcctg caccgacgcc aaaagcgctc tacagcccgt gtgaaattcg ccatctggcg
     6541 attatcgtta acctggaata tgacccgaca gctgcgttcc gcaatcaggt ggtgcatttc
     6601 gatttccgta agctggatgt cttcagcttt ggcgagaatc aaaattgcct ggtaggtagc
     6661 gtggacctgc tgtatcgcaa ctcgtggaac gaagtgcgta cgctgcactt caacggcgag
     6721 cagtcgatga tcgaagccct gaaaactatt ctcggcaaaa tgcatcagga cgccgcaccg
     6781 ccagatagcg tggaagtctt ctgctacagc cagcatctgc gcggattaat ccgtactcgc
     6841 gtgcagcaac tggtttctga gtgcattgaa ctgcgtcttt ccagcacccg ccaggaaacc
     6901 gggcgcttca aggcgctgcg cgtttctggt caaacctggg gcttattctt cgagcgcctg
     6961 aatgtatcgg tacagaaact agaaaatgcc atcgagtttt atggcgctat ttcgcataac
     7021 aaactgcacg gcctgtcagt gcaggttgaa accaatcacg tcaaattacc ggcggtggtg
     7081 gacggctttg ccagcgaagg gatcatccag ttcttcttcg aagaaacgca agacgaaaat
     7141 ggctttaata tctacattct cgacgaaagc aaccgggttg aggtgtatca ccactgcgaa
     7201 ggcagcaaag aggagctggt gcgtgacgtc agtcgcttct actcgtcatc gcacgaccgt
     7261 tttacctacg gctcaagctt catcaacttc aacctgccgc agttctatca gattgtgaag
     7321 gttgatggtc gtgaacaggt gattccgttc cgcaccaaat cgattggcaa catgccgcct
     7381 gccaatcagg atcacgacac gccgctatta cagcagtatt tttcgtgatg agcgtgccgg
     7441 aaagcgaggc ttatccggca tgcaatctta gcggaaactg actgtttcac ccgcttgctg
     7501 cgttgccgcc tgttccagca aatcccagaa ggtttcgccg ctacgatcgc aaatccactc
     7561 atcgcctttc agatcaaaat ggtagccgcc ctgtttggtt gccagccata cctggtgcag
     7621 cggttcctgg cggttgataa tgattttgct gccgttctca aaggtaatgg ttagtacacc
     7681 gccgttgatt tcacagtcga tatcgctgtc gccatcccag tcgtccagac gctcttcaat
     7741 ggtcagccac agttgatcag ccaggcgatg aaattcactg tcgttcattg ttgtatcctg
     7801 tttttaagtg atggcggcag tatagcggca tggggtcagg gcttcaaagt ttgctcgtct
     7861 gctgcgttct ggcatgattc atccgtcaac ggaataataa tgtctctata tagcgaaaga
     7921 ttggtctttt cataagggcg caaatacacc acgccttttc cctgtttctg gtaaatatta
     7981 tgttccgctc atcaagccga catccccatg ccgacgataa aaacaacgaa caagccaaca
     8041 aacaaaccac attgcgatag tgcataaagc catcctggcg cgaggtgccg atcacgaaac
     8101 taccagcaaa acataaatcg ccacgagtaa gcgttatact cgcagcattt cctcactttt
     8161 cagacttcat aaagagtcgc taaacgcttg cttttacgcc ttctcctgcg atgatagaaa
     8221 gcagaaagcg atgaacttta caggcaatcc ataatgaaaa acgtgtttaa ggcactcact
     8281 gtattactta ctctcttcag cctgacgggc tgcggtctga aaggtccgct ctatttcccg
     8341 cctgcagata aaaacgcacc gccgccgacc aaaccggtag agacgcaaac gcaatccacg
     8401 gtgccggata aaaacgaccg cgccactggc gatggtccat cccaggtgaa ttactaaaag
     8461 tcagtttctg tacccgcgtg attggagtaa atgatgcagt tctcgaaaat gcatggcctt
     8521 ggcaacgatt ttatggtcgt cgacgcggta acgcagaatg tctttttttc accggagctg
     8581 attcgtcgcc tggcggatcg gcacctgggg gtagggtttg accagctgct ggtggttgag
     8641 ccgccgtatg atcctgaact ggattttcac tatcgcattt tcaatgctga tggcagtgaa
     8701 gtggcgcagt gcggcaacgg cgcgcgctgc tttgcccgtt ttgtgcgtct gaaagggctg
     8761 accaataagc gcgatatccg cgtcagcacc gccaacgggc ggatggttct gaccgtcacc
     8821 gatgatgatc tggttcgcgt aaacatgggc gagcccaact tcgaaccttc cgctgtgccg
     8881 tttcgcgcta acaaagcgga aaagacctat attatgcgcg ccgccgagca gacaatctta
     8941 tgtggcgtgg tgtcgatggg aaatccgcac tgcgtgattc aggtcgatga tgtcgatact
     9001 gcggcggtag aaacgcttgg tcctgttctg gaaagccatg agcgttttcc ggagcgcgcc
     9061 aatatcggtt ttatgcaagt ggttaagcgc gagcatattc gtttacgcgt ttatgagcgt
     9121 ggggcaggag aaacccaggc ctgcggcagc ggcgcatgtg cggcggtggc ggtagggatc
     9181 cagcaaggtt tgctggccga agaagtacgc gtggaactcc ccggcggtcg tcttgatatc
     9241 gcctggaaag gtccgggtca cccgttatat atgactggcc cggcggtaca tgtctacgac
     9301 ggatttattc atctatgaag caaccagggg aagaactgca ggaaacactc acggagcttg
     9361 atgaccgggc ggttgtcgat tatctgatta aaaatcctga gttttttatc cgcaatgcgc
     9421 gcgcagttga agcgatacgt gtgccgcatc cggtacgcgg caccgtttcg ttggtcgaat
     9481 ggcacatggc ccgcgcacgt aatcatattc atgttctgga agagaacatg gcgctgttga
     9541 tggaacaggc tattgccaac gaaggcctgt tttatcgctt actctacttg cagcgtagtc
     9601 tcaccgccgc cagcagtctc gatgatatgc tgatgcgctt tcaccgctgg gcgcgcgatc
     9661 tcggcctggc aggggcgagt ttgcgcctgt ttccagatcg ctggcgctta ggtgcgccgt
     9721 cgaaccacat tcatctggca ttaagccgtc agtctttcga accgctgcgt attcagcgtc
     9781 tggggcagga acagcactat ctggggccgc ttaacggacc ggagcttctg gtggtgctac
     9841 cggaagcgaa agcggtggga tcggtggcga tgtcgatgct gggaagcgat gctgatttgg
     9901 gtgttgtgct gtttaccagt cgcgatgcca gtcactatca acaagggcag ggaacgcagt
     9961 tacttcatga aattgcgctg atgttgccgg agcttctgga gcgttggatt gaacgcgtat
    10021 gaccgattta cacaccgatg tagaacgcta cctacgttat ctgagcgtgg agcgccagct
    10081 aagcccgata accctgctta actaccagcg tcagcttgag gcgatcatca attttgccag
    10141 cgaaaacggt ctgcaaagct ggcagcaatg tgatgtgacg atggtgcgca atttcgctgt
    10201 acgcagtcgc cgtaaagggc tgggagctgc aagtctggcg ttacggcttt ctgcgctacg
    10261 tagctttttt gactggctgg tcagccagaa cgagctcaaa gccaacccgg cgaaaggtgt
    10321 ttcggcaccg aaagcgccgc gtcatctgcc gaaaaacatc gacgtcgatg atatgaatcg
    10381 gctgctggat attgatatca acgatcccct cgccgtgcgc gatcgggcaa tgctggaagt
    10441 gatgtacggc gcgggtctgc gtctttccga actggtgggg ctcgatatca aacacctcga
    10501 cctggagtct ggtgaagtgt gggtgatggg gaaaggcagc aaagagcgcc gcctgccgat
    10561 tggtcgcaac gctgtggcgt ggattgagca ctggcttgat ttgcgcgacc tgtttggtag
    10621 cgaagacgac gcgctttttc tgtcgaaact gggcaagcgt atctccgcgc gtaatgtgca
    10681 gaaacgcttt gccgaatggg gcataaaaca agggctgaat aatcacgttc acccgcataa
    10741 attacgtcac tcgttcgcta cgcatatgct ggagtcgagc ggcgatcttc gtggtgtgca
    10801 ggagctgctg ggtcatgcca acctctccac cacgcaaatc tatactcatc ttgattttca
    10861 acaccttgcc tcggtgtacg atgcggcgca tccacgcgcc aaacggggga aataatgcgt
    10921 ttttaccggc ctttggggcg catctcggcg ctcacctttg acctggatga taccctttac
    10981 gataaccgtc cggtgatttt gcgcaccgag cgagaggcgc ttacctttgt gcaaaattat
    11041 catccggcgc tgcgcagctt ccagaatgaa gatctgcaac gcctgcgcca ggcggtacgg
    11101 gaagcggaac ccgagattta tcacgacgtg acgcgctggc gttttcgttc gattgaacaa
    11161 gccatgctcg acgccgggct gagtgccgaa gaagccagtg caggcgcaca cgcggcaatg
    11221 atcaactttg ccaaatggcg cagccgaatc gatgtcccgc agcaaaccca cgacacctta
    11281 aaacagctgg cgaagaaatg gccgctggtg gcgatcacca acggtaacgc ccagccggaa
    11341 ctgtttggtt tgggggatta ttttgagttt gtgctgcgcg ctggcccgca cgggcgctca
    11401 aaaccgttca gcgatatgta ctttctggct gcggaaaaac tcaacgtgcc gatcggcgag
    11461 atcttacatg ttggggacga tctcaccact gacgtgggtg gggcaattcg tagcggaatg
    11521 caggcttgtt ggatcagacc ggaaaatggc gatctgatgc aaacctggga cagccgttta
    11581 ctcccgcatc tggaaatttc ccggttggca tctctgacct cgctgatata atcagcaaat
    11641 ctgtatatat acccagcttt ttggcggagg gcgttgcgct tctccgccca acctattttt
    11701 acgcggcggt gccaatggac gtttcttacc tgctcgacag ccttaatgac aaacagcgcg
    11761 aagcggtggc cgcgccacgc agcaaccttc tggtgctggc gggcgcgggc agtggtaaga
    11821 cgcgcgtact ggtgcatcgt atcgcctggc tgatgagcgt ggaaaactgc tcgccatatt
    11881 cgattatggc ggtaacgttt accaacaaag cggcggcgga gatgcgtcat cgtatcgggc
    11941 agctgatggg caccagccag ggcggcatgt gggtcggcac cttccacggg ctggcgcacc
    12001 gtctgctgcg tgcgcaccat atggacgcca atctgccgca ggattttcag atcctcgaca
    12061 gcgaggacca gctacgcctg ctcaaacgcc taatcaaggc gatgaacctc gacgagaagc
    12121 agtggccgcc gcgccaggca atgtggtaca tcaacagcca gaaagatgaa ggcctgcgtc
    12181 cacatcatat tcaaagctac ggtaatccgg tggagcagac ctggcagaag gtgtatcagg
    12241 cgtatcagga agcgtgtgac cgcgcgggcc tggtggactt cgccgagctg ctgctacgtg
    12301 ctcacgagtt gtggcttaac aagccgtata tccttcagca ctatcgcgaa cggtttacca
    12361 atatcctggt ggacgaattc caggatacca acaacattca gtatgcgtgg atccgcctgc
    12421 tagcgggcga taccggcaaa gtgatgatcg ttggcgatga cgatcagtca atctacggct
    12481 ggcgcggagc gcaggtggag aatattcagc gtttcctcaa tgatttcccc ggtgctgaaa
    12541 ctatccgtct ggagcagaac taccgctcta ccagcaatat tctgagcgcc gcgaacgccc
    12601 tgattgaaaa caacaacggg cgtctgggta aaaaactgtg gaccgatggc gcggacggtg
    12661 agccgatttc cctctattgc gcttttaacg aactcgatga agcgcgtttt gtggttaacc
    12721 gtatcaaaac ctggcaggac aaaggcgggg cgcttgccga gtgcgccatt ctctaccgca
    12781 gcaacgccca gtcgcgtgtg ctggaagaag cactgttaca ggcaagtatg ccgtaccgta
    12841 tttacggcgg gatgcgcttc ttcgaacgcc aggaaatcaa agacgcgctc tcgtatctgc
    12901 gcctgattgc caaccgtaat gacgacgcgg cctttgagcg cgtggtgaat acaccaacgc
    12961 ggggtattgg tgaccggacg ctggacgtgg tacgtcagac atcgcgcgat cgccagttaa
    13021 cgctgtggca ggcatgtcgt gaactgttgc aggaaaaagc cctcgccgga cgtgctgcca
    13081 gtgccttaca gcggtttatg gaactgatcg acgccttagc gcaggaaact gccgatatgc
    13141 cgctgcatgt acagactgac cgggtaatta aagactccgg cctgcgcacc atgtacgagc
    13201 aggagaaagg cgaaaaaggc cagacgcgta tcgaaaactt agaggaactg gtaacggcga
    13261 cgcgccagtt tagctacaac gaagaagacg aagatttaat gccgctgcag gcattcctct
    13321 cccatgcggc actggaagcg ggcgaggggc aggcggatac ctggcaggat gcggtgcagt
    13381 tgatgacgct acactcggcg aaagggctgg agtttccgca ggtgtttatc gttggtatgg
    13441 aagaaggcat gttcccaagc cagatgtcgc tggatgaagg cggacgtctg gaagaagaac
    13501 gccgtctggc ctacgttggc gtaacccgcg cgatgcagaa actgacgctg acctacgcgg
    13561 aaacccgccg tctgtatggc aaagaggttt accatcgccc gtcgcgcttt atcggcgagc
    13621 tgccggaaga gtgtgtggaa gaggtgcgcc tgcgcgccac ggtaagccgc ccggtcagcc
    13681 atcaacggat gggtacgccg atggtcgaga acgacagcgg ctacaagctc ggccagcgcg
    13741 tacgccacgc taagtttggt gaaggcacca tcgtcaatat ggaaggcagc ggtgagcata
    13801 gccgtttgca ggtggcattt cagggccagg gtattaaatg gctggtggcg gcttatgccc
    13861 ggttggagac ggtgtgacgt tgcgggatgc ggtgcgccac accgcatccc gcatcagcac
    13921 cttacttagc gcacttcttc acatcaatat tccagtcagc accagacgta acctgatgtt
    13981 tctgcgcgaa attatccata ttcagcgcca cggaaacatt caacaagctg ttcagataca
    14041 ccatcggctg gccttccggc acatcgccga agcttgcgac atacggcatt ttccctgcgt
    14101 atttttgctg ggaaccttcg gaaatggtca cacacaacgt gtcgttgagt ttgattcctg
    14161 cctgattcag caactcgtcg ctgatattgc tccagacgtt gccgtactga atatcgagaa
    14221 tcgggatatt gcccttcact tcaccttttg ttgctttggc tttctgataa gaaagttcga
    14281 caacttttgc aggaagctct ggccccacct gttcgaacgt aatcgccccg gaagccagac
    14341 gcgcaccggt gtaggcatac acatcacgac cgtggaaggt atatgacttt taagaacctt
    14401 tcaggcggtt agctttttcg tcgatttccc ggacgctatc aatacccaga gtttgtgcca
    14461 ccaaagtgag cgtaccgtta tctggtgaaa cgaagtattg accgtttttg gttttcagca
    14521 ctaccgattt acggtcagtg cctacgcccg gatcgaccac gctaacaaac accgaacctt
    14581 gcggccagta actggcagtc tgatacagac gataagcgcc ttcccagatg ttatacggtg
    14641 gaatttcgtg tgttaaatca aagattttaa gattatggtc aacgccgaat gcgacccctt
    14701 tcatcgccga gacggcacca tctttcaggc taaaatcagt ttgtaaaatc agagcgttat
    14761 ccgcataggc ggatgatgat cccagacacg ttaataaaaa taacgttgcg agcgtttttt
    14821 tcatttttgt ttcccttcat ttatctgatg attttccagc tgtgtagcca gtcccagttt
    14881 gtctggcctc ctcgagtgta caatgcttaa cctttccact cgacggaaat catggtgaca
    14941 aaaggatagc gttgccacgg gattgccccg cctttcatat aaatatgtga aattgtgcca
    15001 tcaagataaa gcaactgctc aacgttcagt ttcgctttgg cataacaggc aaaatcataa
    15061 aaatttgtcg cctgctggct caacaaaaac acggcgttcc cttgtttatt aatcccaaca
    15121 ccgttacgaa ttttgcgtga ggcgacgttg ggatgaatac gcggattaat tacaccgttt
    15181 tccatcaaca ttggccctga ctgcaccgca aactgaatat ctttactggc tttaaaggca
    15241 tccagacgaa cgatgccgac tttatctccc gcgacataaa acacgccgcc aggacggata
    15301 aaaaaattcc cttcacctga agcgagattt aacgccacct tctgctgacc gttttcgatg
    15361 tacaaaccga gcggcgcata gctttcatca tagatgccgc cgttcatcgc catctgcacc
    15421 tgaccctgac tattcatatc cgccagcaga gcatgtaacg ttccccacgc ttcgccattg
    15481 gctttttgcc agtacatttt cacccgctct gtttgaggat taacggtata cgcctgtacg
    15541 gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa cagcaaacaa gggaagtaag
    15601 gtgaggacga gaaaaatccg tttgagattc aaggtgatca ttcctttacc aatgagtagc
    15661 cgatgcgcca ttataggtcc tggatgtggg atttttttat cctgttagcg accttgacga
    15721 gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt tctgttgcgt gttgacggca
    15781 aaattttgct ggcgtaacat gcgcgcacga tcactctaag aggacattcg ccttggacac
    15841 acccagtaga tactggctca ctatcctgtc atccaggatc aactcctaag gctatccctt
    15901 tttgctgata gccttagcgg ttgtcagcga cctcaatttt tcccgtcgcg ctgagtcagg
    15961 ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc accgaactgt ccgatatttt
    16021 aagcattggg agtcccggtc atgctgagcg catttcaact ggaaaataac cgactgaccc
    16081 ggctggaagt cgaagagtca caaccccttg taaatgcagt atggattgat cttgtcgaac
    16141 cggacgacga cgagcgactg cgcgtacaat ctgaacttgg ccagagcctg gcaacccgcc
    16201 cggaactgga agacatcgaa gcatcggcac gtttctttga agacgatgac ggcctgcata
    16261 ttcactcctt cttcttcttt gaagatgcgg aagatcacgc cggtaactcc actgtggcat
    16321 ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg tgaactgcct gcattccgtc
    16381 tgtatcgtat gcgtgcccgt agccagtcaa tggtggacgg taacgcctac gagctgctgc
    16441 tggatctgtt cgaaaccaaa atcgaacagt tggcagatga aattgaaaat atctacagcg
    16501 acctggagca gttgagccgg gtaattatgg aagggcatca gggcgatgag tacgacgagg
    16561 cgctctccac gctggcggaa ctggaagata tcggctggaa agttcgcctg tgtctgatgg
    16621 atacccagcg cgcgctcaac ttcctggtac gcaaggcgcg tttaccgggt ggtcaactgg
    16681 agcaggcgcg tgaaatcctg cgagatatcg aatccctgct gccgcataac gaatctctgt
    16741 tccagaaggt gaacttcctg atgcaggcgg cgatgggctt tatcaatatc gagcagaacc
    16801 gcatcatcaa aatcttctcg gtggtatccg tcgtgttcct gccgccaaca ctggttgctt
    16861 ccagctacgg gatgaacttt gagtttatgc cggaactgaa gtggagtttc ggctaccctg
    16921 gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta tttgtacttt aagcggaaga
    16981 actggttgta aaaaacgaga gcggtggctt agcctggcta agccacctgt tattcaaagg
    17041 ctccaggtat ttaacccttt tacctctttc tcataaaacc atttgttcgt gttaacagca
    17101 acataggctg ctacggcaat tcccagaatg ttaacgccaa ttagtgcacc aacaaataga
    17161 gatagaatgc caagtaatag aactataatt gcttttttcc ataatcccag aacaaataaa
    17221 tagatccagg aacagaagaa agcaatgaaa ttcattttaa tagttaatcg ttgtcttact
    17281 tttaatgttt taaatgctgc tttatattct ggtgttgccc cccagaatcc aggaaagcca
    17341 tgttgatcat aaaagttaaa tcggtatttc catttttcac ttaatgaacc atcgttcata
    17401 tactccttac tcataaatac tccataacta ttgttttgat gaatcagtag gtgcaagcat
    17461 tagcatactg aaagtggaaa aataacaaat caaaaaaatc atcgaaccat tgcctgaaca
    17521 atcaaaatgt tcggctatca ttgtgatgat agagatgata tatactgcta atgtaccaaa
    17581 aacataagtt tttatatata tgaaaccact atcacggagt cgttggcaat ttatgttgat
    17641 gacgagataa tggagtgcga tggtagagac aataacaaga aaacctgcgt ccccatcgtt
    17701 aaaaaagata ataagaaagg caaaaatgaa atttattaaa ataaatgaaa atatataacg
    17761 acgtctggaa atcttaccgt tagatgttgg gataaatata cgcaacataa attttacatc
    17821 cttgtgtgag tctcctgtca gcatggcaat atgcccactc tcatgcaaga gtcggcatat
    17881 tttttcagaa tatatttatt ttttatttgg acgttctacg ctgcgtataa atcgcatcca
    17941 tcacaaaaat tgccagcgcc acccaaataa aggcgaaggt caccatctta tcggcacccg
    18001 gtttttcgcc ataaaacgtc acagccagca ggaacatcag cgtcgggcca atgtactgaa
    18061 aaaagcctaa cgttgagaga cgcaggcgtg tcgcagccgc agtaaaacac aacagcggta
    18121 cggtagtgac aataccggcg gcgatcagca gtaaattcag cgacatcggg ttttgcccca
    18181 tatggctggt tgcgctgtcg gcaatagcaa acaggtaaat cgccgccacg ggcagcaacc
    18241 acatagtttc gattaacatg ccggtttgcg cttcaacagc aatcttcttg cgtaccaggc
    18301 cgtagaaggc aaaactaaac gccagcccca gcgcgataat cggcagtgaa ccaaaagtcc
    18361 acaactgaac caacacgcca catatcgcca gaatcaccgc cagccattgc atccggcgga
    18421 atcgctcgcc gaggaaaatc atccccagca caatgttcac cagcgggtta ataaagtaac
    18481 caaggctcgc ttccagcata tggtgattgt tcaccgccca gataaacagt agccagttgc
    18541 caccaatcag cacggcagag actgccagca taaaaatttt ctgtggcgtc tgaatcagcg
    18601 tttttaaata ggaccactgg cggcaaatgc tcatcagcac caccataaag aaaaacgacc
    18661 agatcacgcg atgcgtcagg atctcatcgg cgggcacgta gtaaatcagc ttgaagtacg
    18721 ctggcgcaat accccaaata aaataagcgg caagagcgag taatacgccc tgccgcgttt
    18781 gttttgcatc catcgggaat actcattttt aattggtaac agcagtttac ctgcttttac
    18841 gttatcaacc taccatatag gtggctgtag cactggcaat ataaagctgt tcttcattgt
    18901 gtaattcaac gcgggcgacg gcgactttat tgcctgcacg taacaggcta ctggtagcag
    18961 taaaacgctc gcctctgcct gggcgcagat aatcgacgcg aagatcaatg gtccccatcc
    19021 gcgatagccg ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt aaggtacttc
    19081 ccacgcacac cagacctgcg gcgacatcca gcgccgacgc aatgaccccg ccgtgcaaaa
    19141 tgctttgcgc ccagttaccc accatcattg gctgattttt aaaggccagc tgtgcgaact
    19201 ctttttcgta acgctccagt tccatcccca atgcgcggtt aaatggcatg tgataaacaa
    19261 acatctcacc cactaatttc agggcttgtt cagcggtcag tacggcagac atatcatcct
    19321 tacacttcat tagttaatga aatgttgatt ttatgcttct ttgttgttgg tttctacttt
    19381 aggaagggat aactaacggc tatggagtta agtatgtaaa atagcccgca gaaaaatatt
    19441 caccttatca ataattcgtt acggagaaca cgagcgatgc ggactctgca gggctggttg
    19501 ttgccggtgt ttatgttgcc tatggcagta tatgcacaag aggcaacggt gaaagaggtg
    19561 catgacgcgc cagcggtgcg tggcagtatt atcgccaata tgctgcagga gcatgacaat
    19621 ccgttcaccc tttatcctta tgacaccaac tacatcatct atacccaaac cagcgatctg
    19681 aataaagaag cgattgccag ttacgactgg gcggaaaatg cgcgtaagga tgaagtaaag
    19741 tttcagttga gcctggcgtt tccgctgtgg cgtgggattt taggcccgaa ctcggtattg
    19801 ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca atagcgaaga gtcttcaccg
    19861 tttcgtgaaa ccaactacga accgcaattg ttcctcggtt ttgccaccga ttaccgtttt
    19921 gcaggttgga cgctgcgcga tgtggagatg gggtataacc acgactctaa cgggcgttca
    19981 gacccgactt cccgtagctg gaaccgcctt tatacccgcc tgatggcaga aaacggtaac
    20041 tggctggtag aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa cccggatatc
    20101 accaaatata tgggttacta ccagcttaaa atcggctatc acctcggtga tgcggtgttg
    20161 agtgcgaaag gacagtacaa ctggaacacg ggctacggcg gcgcggagtt aggcttaagt
    20221 tacccgatca ccaaacatgt gcgcctttat actcaggttt acagcggtta cggcgaatcg
    20281 ctcatcgact ataacttcaa ccagacccgc gttggtgtag gggttatgct aaacgacttg
    20341 ttttgatgaa cggttgagtg gttggcaaat ctggaatcca gtatccagga ttaccctctc
    20401 agagactaaa agcattgcag tttctcgcgc aggcgctgaa aatagcgcct gtttttattt
    20461 caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc cggagctaaa
    20521 caggttttac aagaaacctt tggctaccaa cagtttcgcc ccggccagga agaaattatc
    20581 gacactgtgc tttccggccg cgattgcctc gtcgtcatgc ccactggtgg cggaaaatcc
    20641 ctttgctatc aaatccctgc cttattgcta aacggcctta ccgtggttgt ttcaccgctg
    20701 atttcgttga tgaaagatca ggtggatcaa ctgcaagcca acggcgtggc ggcggcgtgc
    20761 cttaactcga cgcaaacccg cgagcagcaa cttgaagtta tgacaggctg tcgcaccggg
    20821 caaatccgcc tgctttatat cgcgccggaa cgtctgatgc tggataactt tcttgagcat
    20881 ctggcgcact ggaatccggt gttattagcc gttgatgaag cacactgtat ctcccaatgg
    20941 ggtcacgatt tccgcccgga atacgctgcc ctcggtcagc tgcgtcagcg ttttccgacg
    21001 ctgccgttta tggcgctgac tgccacagcc gatgacacca cgcgccagga tatcgtgcgc
    21061 ctgctggggc tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc gaatattcgc
    21121 tacatgctga tggagaaatt caaaccgctc gatcagttga tgcgttatgt acaggaacaa
    21181 cgcggtaagt cggggattat ttactgtaat agtcgtgcga aagtagaaga caccgctgcg
    21241 cgcctgcaaa gcaagggaat tagcgcggcg gcctatcatg ccgggctgga aaataatgtt
    21301 cgcgctgatg tgcaggagaa attccagcgc gatgacctgc aaattgtggt ggcgacggtg
    21361 gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg tggtgcattt tgatattccg
    21421 cgcaacattg aatcctatta tcaggaaacc ggacgcgccg ggcgtgatgg cctgcccgcg
    21481 gaagcgatgc tgttttacga tccggcggat atggcgtggc tgcgtcgttg tctggaagag
    21541 aaaccgcagg ggcagttaca ggatatcgaa cgtcataaac tcaatgcgat gggcgcgttt
    21601 gccgaagcgc aaacttgccg tcgtctggtg ctgctgaact actttggcga agggcggcag
    21661 gagccgtgcg ggaactgcga tatctgcctc gatccgccga aacaatacga tggttcaacc
    21721 gatgctcaga ttgccctttc caccattggt cgtgtgaatc agcggtttgg gatgggttat
    21781 gtggtggaag tgattcgtgg cgctaataac cagcgtatcc gcgactatgg tcatgacaaa
    21841 ctgaaagtct atggcatggg ccgtgataaa agccatgaac attgggtgag cgtgatccgc
    21901 cagctgattc acctcggcct ggtgacgcaa aatattgccc agcattctgc cctacaactg
    21961 acagaggctg cgcgtccggt gctgcgcagc gaatcctctt tgcaacttgc cgtgccgcgt
    22021 atcgtggcgc tcaaaccgaa agcgatgcag aaatccttcg gcggcaacta cgatcgcaaa
    22081 ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg atgaaagcaa tgtcccgccg
    22141 tacgtggtgt ttaacgacgc gaccttgatt gagatggctg aacagatgcc gatcactgcc
    22201 agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt tggcaaaccg
    22261 ttcatggcgc tgattcgtgc gcatgttgat ggcgatgacg aagagtagtc agcaggataa
    22321 aaaagtgcca ggatgaagac tccgtaaacg ttttccccgc gagtctaatg tatgttgatg
    22381 ttattcctca ccgtcgccat ggtgcacatt gtggcgctta tgagccccgg tcccgatttc
    22441 ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag aagcgatgat gggcgtgctg
    22501 ggcattacct gcggcgtaat ggtttgggct gggattgcgt tgcttggcct gcatttgatt
    22561 atcgaaaaaa tggcctggct gcatacgctg attatggtgg gcggtggcct gtatctctgc
    22621 tggatgggtt accagatgct acgtggtgca ctgaaaaaag aggctgtatc tgcgcctgca
    22681 ccacaggttg agctggcgaa aagcgggcgc agtttcctga aaggcttact gaccaatctc
    22741 gccaatccga aagcgattat ctactttggt tcggtattct cgctgtttgt cggcgataac
    22801 gtcggcacca ctgagcgctg gggcattttc gcgttgatta ttatcgaaac actggcgtgg
    22861 tttaccgtcg ttgccagcct gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg
    22921 gcgaagtgga ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt
    22981 atttcgcgtt gatgccagac gcgtcatcag agtaagtcgg ataaggcgtt tacgccgcat
    23041 ccgacattat ttttcacgca tgcctcgccg atgctaacag cgctcccacc agcataaaca
    23101 acgagccgaa aattttattc agcgccttca tctgctttgg tcctttaatc catagagcaa
    23161 tccgttgggc aagggtggcg taaccgatca tcacaataat atcgaccaca atagtcgtga
    23221 cgccgagcac gatatactgc atcagctgcg gctgttgcgg catgatgaat tgcggaaata
    23281 gcgccgccag aaacacaata cttttgggat tggtgagatt cacaaaaact gcgcgctgga
    23341 acaaatgtcg acgcgattga gtagaggcca gcgatttaag atcaattgcg ccagcggcgc
    23401 gccactgctg aatcccaagc caaatcagat aagctgcgcc cgcccacttc aacacttcaa
    23461 acgcaatcac tgagcgggaa aatagcgtcc ccaaacccac gccaaccagc acaatatgaa
    23521 tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc gccgcgataa ccatggttga
    23581 gcgaggtggt catggtgttg attgcgccag agcctggcga caggcttaaa atgatcgatg
    23641 tcagcaggta ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt gtctattttt
    23701 gtgccacaat acgctactgt cgcagcgttg tgtcaggcac gcttaaaaaa acgattttac
    23761 gtggtttaag aggcagatta cccgatgttt cagcagcaaa aagactggga aacaagagaa
    23821 aacgcgtttg ctgcttttac catgggaccg ctgactgatt tctggcgtca gcgagatgaa
    23881 gcagagttta ctggtgtgga tgacattccg gtgcgctttg tccgttttcg cgcacagcac
    23941 catgaccggg tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa
    24001 ctggcctacg acctgttcca tttggggttt gatgtcttaa tcatcgacca tcgcgggcag
    24061 ggacgttccg gtcgcctgtt agccgatccg catctcgggc atgttaatcg ctttaatgat
    24121 tatgttgatg atctggcggc attctggcaa caggaggttc agcccggtcc gtggcgtaaa
    24181 cgctttatac tggcgcactc gatgggcggt gcgatctcca cattatttct gcaacgccat
    24241 ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg
    24301 ccgtcattta tggcacggca gatcctcaac tgggccgaag cgcatccacg tttccgcgat
    24361 ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc
    24421 cacagcagac agcgatatcg acgtaactta cgcttttatg ctgatgaccc aacgattcgc
    24481 gtcggtgggc cgacctacca ttgggtacgc gaaagtattc tggctggcga acaggtgtta
    24541 gccggtgcgg gtgatgacgc cacgccaacg cttctcttac aagctgaaga ggaacgcgtg
    24601 gtggataacc gcatgcatga ccgtttttgt gaactccgca ccgctgcggg ccatcctgtc
    24661 gaaggaggac ggccgttggt aattaaaggt gcgtaccatg agatcctttt tgaaaaggac
    24721 gcaatgcgct cagtcgcgct ccacgctatc gttgattttt tcaacaggca taactcaccc
    24781 agcggaaacc gctctacaga ggtttaaatt tcttatgtac caggttgttg cgtctgattt
    24841 agatggcacg ttactttctc ccgaccatac gttatcccct tacgccaaag aaactctgaa
    24901 gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt
    24961 ggggcaaatt cgcgataatt tggagattaa gtcttacatg attacctcca atggcgcgcg
    25021 cgttcacgat ctggatggca atctgatttt tgctcataac ctggatcgcg acattgccag
    25081 cgatctgttt ggcgtagtca acgacaatcc ggacatcatt actaacgttt atcgcgacga
    25141 cgaatggttt atgaatcgcc atcgcccgga agagatgcgc ttttttaaag aagcggtgtt
    25201 caaatatgcg ctgtatgagc ctggattact ggagccggaa ggcgtcagca aagtgttctt
    25261 cacctgcgat tcccatgaac aactgctgcc gctggagcag gcgattaacg ctcgctgggg
    25321 cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg gaagtgatgg cgggcggcgt
    25381 gtcgaaaggc catgcgttgg aagcagtggc gaagaaactg ggctacagcc tgaaggattg
    25441 tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg tcgatggcgg ggaaaggctg
    25501 cattatgggc agtgcgcacc aacggctgaa agatctgcat ccggagctgg aagtgattgg
    25561 tactaacgcc gaggacgcag tgccgcatta tctgcgtaag ctctatttat cgtaatcgtt
    25621 ctttatttgg tcagttgtca acctgatact tcgctacaat ggatactcgt taatcaaaga
    25681 gttttccatt gtggcgctac ttatcatcac cacgattctg tgggccttct cctttagctt
    25741 ttatggcgag taccttgcag ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg
    25801 cctggcggca ctcgtttttc tgccgtttct gcgtacccgt ggcaatagcc tgaaaacggt
    25861 cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc tgagttttcg
    25921 cgcctatctc tacctgacgg tctccgagct gctgctgttc accgtactga cgccgctcta
    25981 catcacgttg atttatgaca tcatgagtca gcgccgtctg cgctggggct atgcttttag
    26041 cgccttgttg gcggtgattg gtgccgggat tattcgctat gatcaggtca ccgaccactt
    26101 ctggactggc ctgctgctgg tgcagctctc caatatcact tttgccatcg gcatggtggg
    26161 ttacaaacgt ctgatggaaa ctcgcccgat gccgcagcat aacgcctttg cgtggttcta
    26221 tcttggcgcg ttcctggtgg cggtgattgc atggttcttg ctgggaaatg cgcagaaaat
    26281 gccacaaacc acgctgcaat ggggcattct ggtgtttctt ggcgtggtgg cttccgggat
    26341 tggctacttt atgtggaact acggcgcaac ccaagtagac gccggaacgc tgggcattat
    26401 gaataatatg cacgttccgg cagggctgct ggtaaacctg gctatctggc accaacagcc
    26461 gcactggcca acgtttatta caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg
    26521 taagtgggtc gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct
    26581 gagcgaataa acgcttctgt aatcggctga cgctgctcgc catcacgcac ggcggcgtac
    26641 agtcggctcc acaagccttc gcccagggtt ttggtcacca ccagaccctg gcgttcaaaa
    26701 ctctccacca cccaatgcgg tagtgcggca atacccatcc gtgcggcaac catctgaatc
    26761 aacaataagg tgttatcgac gcttttcagt gacgggctga tgcctgctgg ctgaagaaaa
    26821 tgccgccaga catccagtcg actacgctgc accggataaa tcaacagcgt ctcgctggcg
    26881 agatcttccg gtgtaatacg cgttttcgcc gccagtggat ggtcaggtgc caataccaga
    26941 cgcacttcat aatcgaacat cggcgaataa tgcaggccac tgcgcggcag aatatcggac
    27001 gtcattacca gatccagctc tccctgttgc aaggcgggct gcgggtcaaa tgtcacgccc
    27061 gatttaaagt ccatctcgac ctgcggccag ttcttatgga aattttctaa cgcgggcgtc
    27121 agccactgaa tacagctatg gcactcaatg gcgatgcgca gacgcgtctg ctgcggttca
    27181 ttgcaggctt gcagtgcctg gctaatttgt ggcagcacct ggtttgccag ttgcaacagg
    27241 atttctccct gcggtgtaaa gcgaagcggc tggctcttac gcacaaatag ccggaagcca
    27301 aggcgttgtt ccagatcgct aaactggtga gatagggcgg attgcgtctg atgcaacgtc
    27361 gccgcagcgg ctgcgagaga gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt
    27421 acttcgatca tgaaagtcct tcacttcggc atgaataatt tgcgcttgag gaatatacag
    27481 taaccgccaa ttatggatgt gtaaacatct ggacggctaa aatccttcgt cttttaaatt
    27541 tatggtgcgt tggctgcgtt tcttcacccc ggtcacttac ttcagtaagc tcccggggat
    27601 gaataaactt gccgccttcc ctaaattcaa aatccatagg atttacatat aattagagga
    27661 agagaaatga caatattgaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag
    27721 ctgaaaaaag cgcaagagag ttattgggcg gggaactcca cgcgtgaaga actgctggca
    27781 gtagggcgtg aactgcgtgc ccgtcactgg gatcaacaaa agcaggcggg tatcgacctg
    27841 ctgccggtgg gcgattttgc ctggtacgat catgtactga ccaccagtct gctgctgggt
    27901 aatgttccgc cacgtcatca gaacaaagat ggttcggtag atatcgacac cctgttccgt
    27961 attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg
    28021 tttaacacca actatcacta catggtgccg gagttcgtta aaggccagca gttcaaactg
    28081 acctggacgc agctgctgga ggaagtggac gaggcgcagg cgctgggcca caaggtgaaa
    28141 cctgtgctgc tggggccggt tacctaccta tggcttggga aagtgaaagg tgaacagttt
    28201 gaccgcctga gcctgctaaa cgacattctg ccggtttatc agcaagtact ggcagaactg
    28261 gcaaaacgcg gcattgagtg ggtacagatc gacgaacccg cactggtact ggaactaccg
    28321 caggcgtggc tggacgcata caaacccgct tacgacgcgc ttcagggcca ggtgaaactg
    28381 ctgctgacta cctattttga aggcgtaacg ccaaatctcg acacgattac tgcgctgcct
    28441 gttcaggggc tgcatgtgga ccttgtacat ggtaaagatg acgttgctga actgcacaag
    28501 cgtctgcctt cagactggct actgtctgcg ggtcttatca atggtcgtaa cgtctggcgc
    28561 gccgatctta ccgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg
    28621 gtggcatctt cctgctcgtt gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt
    28681 gatgcagaag tgaaaagctg gtttgccttc gccctacaaa aatgccatga actgtcactg
    28741 ctgcgcgatg cgctgaacag tggtgatacg gcagctctgg cagagtggag cgccccgatt
    28801 caggcgcgtc gtcactcaac ccgcgtacat aatccggcag tagaaaagcg tctggcggcg
    28861 atcaccgctc aggacagtca gcgtgcgaat gtctatgaag tgcgtgctga agcccagcgt
    28921 gcgcgtttta aactcccagc atggccgacc accacgattg gttctttccc gcaaactacg
    28981 gaaattcgta ccctgcgtct ggatttcaaa aagggtaatc tcgacgctaa caactaccgc
    29041 acgggcattg cggaacatat caagcaggcc attgttgagc aggaacgttt gggactggat
    29101 gtgctggtac atggcgaggc tgagcgtaat gacatggtgg aatactttgg cgagcacctc
    29161 gacggttttg tctttacgca aaacggttgg gtacagagct acggttcccg ctgcgtgaag
    29221 ccaccgattg ttattggtga cgttagccgc ccggcaccga ttaccgtgga gtgggcaaaa
    29281 tatgcgcaat ccctgactga taaaccggtg aaagggatgt tgaccggccc agtgaccatt
    29341 ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa ccatcgccaa acaaattgcg
    29401 ctggcgctgc gtgatgaagt cgcggacctg gaagccgctg gaattggcat cattcagatt
    29461 gatgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta gcgactggga tgcatatctc
    29521 cagtggggcg tcgaggcttt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc
    29581 cacactcaca tgtgttactg cgagttcaac gacatcatgg attcgattgc ggcgctggac
    29641 gcagacgtca tcaccatcga aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa
    29701 gagtttgatt atccaaatga aatcggtcct ggcgtctatg acattcactc gccaaacgta
    29761 ccgagcgtag aatggattga agccttgctg aagaaagcgg caaaacgcat tccggcagag
    29821 cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg
    29881 gcactggcga acatggtgca ggcggcgcag aatttgcgtc gggggtaaat ccaaaccggg
    29941 tggtaatacc acccggtctt ttctcattac agcgacttct tcccaccata ccgcttaaac
    30001 cactccagca tacgctgcca gccgtctttc gcagattcgg catgatagct cgggcgataa
    30061 tcagcgttga atgcatgtcc ggcatccggg tacacgataa tctctgcttt cgcattagca
    30121 gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg gaatgctgtt atcctgacca
    30181 ccatataagc cgagaaccgg cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt
    30241 gaattcagcg acttatcgcc cgtcagtttg ccgtaccatg ccaccgccgc tttcagttgc
    30301 gggttatgcg cagcatacag ccaggtgata cgtccacccc agcagaatcc ggtgatcatt
    30361 aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg cgacatgatc gagatcggcc
    30421 agcacctgcg agtcaggcac ttttgctacc agaccgctaa gtaacgtggg gatatcggca
    30481 aaatcattcg gatcgccttc gcggaagtaa agttcaggag cgatagccag atacccctcc
    30541 agcgccagac ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat ttcctgcact
    30601 acaatgacca ctggcagtgg gccatcgctt tgctttggtc tggcatggta agcaggcatg
    30661 ttatcccctt gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc cggggtctga
    30721 acgatggtcg aagctagagg cgatgcagca ggtgcaaatc cagattgttg tgttgttgcc
    30781 atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa
    30841 caaatcacat tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt
    30901 tatgtgattt gcatcacttt tggcgggtaa atttatgcaa cgcattttcg tcatggtgat
    30961 gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa
    31021 accgattcac agaggagttg tatatgtcca agtctgatgt ttttcatctc ggcctcacta
    31081 aaaacgattt acaaggggct acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa
    31141 agatcgccgc gctgatggat aagccggtta agctggcatc tcaccgcgaa ttcaccacct
    31201 ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc taccggtatc ggcggcccgt
    31261 ctacctctat tgctgttgaa gagctggcac agctgggcat tcgcaccttc ctgcgtatcg
    31321 gtacaacggg cgctattcag ccgcatatta atgttggcga tgttctggtt accacggcgt
    31381 ctgttcgtct ggatggcgcg agcctgcact tcgcaccgct ggaattcccg gctgtcgctg
    31441 atttcgaatg tacgactgcg ctggttgagg ctgcgaaatc catcggcgcg acgactcacg
    31501 ttggcgtgac ggcttcttct gataccttct acccaggtca ggaacgttac gatacttact
    31561 ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg atgggcgtaa
    31621 tgaactatga aatggaatct gcaaccctgc tgaccatgtg tgcaagtcag ggcctacgtg
    31681 ccggtatggt agcgggtgtt atcgttaacc gcacccagca agagatcccg aatgctgaga
    31741 cgatgaaaca aactgaaagc caagcggtga aaatcgtggt ggaagcggcg cgtcgtctgc
    31801 tgtaatcctc ttctcctgtc tgaaggccga cgcgttcggc cttttgtatt tttgcgtagc
    31861 gcctcgcagg aaatgccttt ccaactggac gtttgtacag cacaattcta ttttgtgcgg
    31921 gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc atggtttacg cagttattgc
    31981 gttggtgggt gtggcaattg gctggctgtt tgccagttat caacatgcgc agcaaaaagc
    32041 cgagcaatta gctgaacgtg aagagatggt cgcggagtta agcgcggcaa aacaacaaat
    32101 tactcaaagc gagcactggc gcgctgagtg cgagttactc aataacgaag tgcgcagcct
    32161 gcaaagtatt aacacctctc tggaggccga tctgcgtgag gtgaccacgc ggatggaagc
    32221 cgcacagcaa cacgctgacg ataaaattcg ccagatgatt aacagcgagc agcgcctcag
    32281 tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac agcaatcgcc gggttgatga
    32341 gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta cgtgaacaac tggacggttt
    32401 ccgccgtcag gttcaggaca gctttggtaa agaagcacaa gaacgccata ccctgaccca
    32461 cgaaattcgc aatctccagc agctcaacgc gcaaatggcc caggaagcga tcaacctgac
    32521 gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg ggcgaggtag tattgacgcg
    32581 ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat gaaacccagg tcagcatcga
    32641 aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg cggttgccgc agggaaaaga
    32701 tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa cgctatttca acgccgaaga
    32761 tgactacacc cgcgaaagcg cgctacagga acatatcgcg tcggtgcgta accatatccg
    32821 tttgctggga cgcaaagatt atcaacagct gccggggctg cgaaccctgg attacgtgct
    32881 gatgtttatt cccgttgaac ccgctttttt actggcgctt gaccgccagc cggagctgat
    32941 caccgaagcg ttgaaaaaca atatcatgct ggttagcccg actacgctgc tggtggcgct
    33001 gcgcactatc gccaacctgt ggcgttatga gcatcaaagc cgcaacgccc agcaaatcgc
    33061 cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc atcgatgaca tgtccgcgat
    33121 tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag gcaatgaaaa aactctcttc
    33181 aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc ggtttagggg tagaaattaa
    33241 acgcgagatt aatccggatt tggctgaaca ggcggtgagc caggatgaag agtatcgact
    33301 gcggtcggtt ccggagcagc cgaatgatga agcttatcaa cgcgatgatg aatataatca
    33361 gcagtcgcgc tagcccattg ggggtagtta agccgggtag aaatctaggg catcgacgcc
    33421 caatctgtta cacttctgga acaatttttt gatgagcagg cattgagatg gtggataagt
    33481 cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa ggaacaaaaa gcggatatgg
    33541 tcgctcacgt tttccattcc gtggcatcaa aatacgatgt catgaatgat ttgatgtcat
    33601 tcggtattca tcgtttgtgg aagcgattca cgattgattg cagcggcgta cgccgcgggc
    33661 agaccgtgct ggatctggct ggtggcaccg gcgacctgac agcgaaattc tcccgcctgg
    33721 tcggagaaac tggcaaagtg gtccttgctg atatcaatga atccatgctc aaaatgggac
    33781 gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga gtatgttcaa gcgaacgctg
    33841 aggcgctgcc gttcccggat aacacctttg attgcatcac catttcgttt ggtctgcgta
    33901 acgtcaccga caaagataaa gcactgcgtt caatgtatcg cgtgctgaaa cccggcggcc
    33961 gcctgctggt gcttgagttc tcgaagccga ttatcgagcc gctgagcaaa gcctatgatg
    34021 catactcctt ccatgtgctg ccgcgtattg gctcactggt cgcgaacgac gccgacagct
    34081 atcgttatct ggcagaatcc atccgtatgc atcccgatca ggataccctg aaagccatga
    34141 tgcaggatgc cggattcgaa agtgtcgact actacaatct gacggcaggg gttgtggcgt
    34201 tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc cttttaaacc tttagtgacg
    34261 gcaggaattg aaagtctgct caacaccttc ctgtatcgct cacccgcgct gaaaacggcc
    34321 cgctcgcgtc tgttgggcaa agtgctgcgc gtggaggtaa agggcttttc gacgtcattg
    34381 attctggtgt tcagcgaacg ccaggttgat gtactgggcg aatgggcagg cgatgctgac
    34441 tgcaccgtta tcgcttacgc cagtgtgttg ccgaaacttc gcgatcgcca gcagcttgcc
    34501 gccctgattc gcagtggtga gctggaagtg cagggcgata ttcaggtggt gcaaaacttc
    34561 gttgcgctgg cagatctggc agagttcgac cctgcggaac tgctggcccc ttataccggt
    34621 gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag gcgcaaagtt cctgcatcac
    34681 ggcattaagc gccagcaacg ttatgtggca gaagccatta ctgaagagtg gcgtatggca
    34741 cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg ctgccgtcga gcgtgctgtt
    34801 gatgccctga ccaaacggct ggaaaaactg gaggctaaat gacgccaggt gaagtacggc
    34861 gcctatattt catcattcgc acttttttaa gctacggact tgatgaactg atccccaaaa
    34921 tgcgtatcac cctgccgcta cggctatggc gatactcatt attctggatg ccaaatcggc
    34981 ataaagacaa acctttaggt gagcggctac gactggccct gcaagaactg gggccggtat
    35041 ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct ttttccgccg catattgccg
    35101 atcagctggc gttattgcag gacaaagtcg ctccgtttga tggcaagctg gcgaagcagc
    35161 agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg gtttgacgat tttgaaatca
    35221 agccgctggc ttctgcttct atcgcccagg ttcataccgc gcgattgaaa tcgaatggta
    35281 aagaggtggt gattaaagtc atccgcccgg atattttgcc ggttattaaa gcggatctga
    35341 aacttatcta ccgtctggct cgctgggtgc cgcgtttgct gccggatggt cgccgtctgc
    35401 gcccaaccga agtggtgcgc gagtacgaaa agacattgat tgatgaactg aatttgctgc
    35461 gggaatctgc caacgccatt cagcttcggc gcaattttga agacagcccg atgctctaca
    35521 tcccggaagt ttaccctgac tattgtagtg aagggatgat ggtgatggag cgcatttacg
    35581 gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg cactaacatg aaattgctgg
    35641 cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg cgacagcttt ttccatgccg
    35701 atatgcaccc tggcaacatc ttcgtaagct atgaacaccc ggaaaacccg aaatatatcg
    35761 gcattgattg cgggattgtt ggctcgctaa acaaagaaga taaacgctat ctggcagaaa
    35821 actttatcgc cttctttaat cgcgactatc gcaaagtggc agagctacac gtcgattctg
    35881 gctgggtgcc accagatacc aacgttgaag agttcgaatt tgccattcgt acggtctgtg
    35941 aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg acatgtactg ttaaatctgt
    36001 ttaatacggc gcgtcgcttc aatatggaag tgcagccgca actggtgtta ctccagaaaa
    36061 ccctgctcta cgtcgaaggg gtaggacgcc agctttatcc gcaactcgat ttatggaaaa
    36121 cggcgaagcc tttcctggag tcgtggatta aagatcaggt cggtattcct gcgctggtga
    36181 gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat gccagaactg cctgaactgg
    36241 tttacgacag tttgcgccag ggcaagtatt tacagcacag tgttgataag attgcccgcg
    36301 agcttcagtc aaatcatgta cgtcagggac aatcgcgtta ttttctcgga attggcgcta
    36361 cgttagtatt aagtggcaca ttcttgttgg tcagccgacc tgaatggggg ctgatgcccg
    36421 gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg ttggcgcaaa acacgctgat
    36481 tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg gccttgttta atcatcatct
    36541 accacagagg aacatgtatg ggtggtatca gtatttggca gttattgatt attgccgtca
    36601 tcgttgtact gctttttggc accaaaaagc tcggctccat cggttccgat cttggtgcgt
    36661 cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc aaagcaggat aaaaccagtc
    36721 aggatgctga ttttactgcg aaaactatcg ccgataagca ggcggatacg aatcaggaac
    36781 aggctaaaac agaagacgcg aagcgccacg ataaagagca ggtgtaatcc gtgtttgata
    36841 tcggttttag cgaactgcta ttggtattca tcatcggcct cgtcgttctg gggccgcaac
    36901 gactgcctgt ggcggtaaaa acggtcgcgg gctggattcg cgcgttgcgt tcactggcga
    36961 caacggtgca gaacgaactg acccaggagt taaaactcca ggagtttcag gacagtctga
    37021 aaaaggttga aaaggcgagc ctcactaacc tgacgcccga actgaaagcg tcgatggatg
    37081 aactacgcca ggccgcggag tcgatgaagc gttcctacgt tgcaaacgat cctgaaaagg
    37141 cgagcgatga agcgcacacc atccataacc cggtggtgaa agacaatgaa gctgcgcatg
    37201 aaggcgtaac gcctgccgct gcacaaacgc aggccagttc gccggaacag aagccagaaa
    37261 ccacgccaga gccggtggta aaacctgctg cggacgctga accgaaaacc gctgcacctt
    37321 ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa gatactcaac cgcttatcac
    37381 gcatctgatt gagctgcgta agcgtctgct gaactgcatt atcgcggtga tcgtgatatt
    37441 cctgtgtctg gtctatttcg ccaatgacat ctatcacctg gtatccgcgc cattgatcaa
    37501 gcagttgccg caaggttcaa cgatgatcgc caccgacgtg gcctcaccgt tctttacgcc
    37561 gatcaagctg acctttatgg tgtcgctgat tctgtcagcg ccggtgattc tctatcaggt
    37621 gtgggcgttt atcgccccag cgctgtataa gcatgaacgt cgcctggtgg tgccgctgct
    37681 ggtttccagc tctctgttgt tttatatcgg catggcgttc gcctactttg tggtctttcc
    37741 gctggcattt ggcttccttg ccaataccgc gccggaaggg gtacaggtat ccaccgacat
    37801 cgccagctat ttaagcttcg ttatggcgct gtttatggcg tttggtgtct cctttgaagt
    37861 gccggtggca atagtgctgc tgtgctggat ggggattacc tcgccagaag acttacgcaa
    37921 aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg atgctgctga caccgccgga
    37981 tgtcttctcg caaacgctgt tggcgatccc gatgtactgc ctgtttgaaa tcggtgtctt
    38041 cttctcacgc ttttacgttg gtaagggtcg aaaccgagaa gaagaaaacg acgctgaagc
    38101 agaaagcgaa aaaactaaag aataaactca accgcccgtc agggcggttg tcatatggag
    38161 ttcaggatgt ttgatatcgg cgttaatttg accagttcgc aatttgcgaa agaccgtgat
    38221 gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc tactcatcac cggcactaac
    38281 ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt attcgtcctg ttggtcaacg
    38341 gcgggcgtac atcctcacga cagcagccag tggcaagctg cgactgaaga agcgattatt
    38401 gagctggccg cgcagccaga agtggtggcg attggtgaat gtggcctcga ctttaaccgc
    38461 aacttttcga cgccggaaga gcaggaacgc gcttttgttg cccagctacg cattgccgca
    38521 gaattaaaca tgccggtatt tatgcactgt cgcgatgccc acgagcggtt tatgacattg
    38581 ctggagccgt ggctggataa agtgcctggt gcggttcttc attgctttac cggcacacgc
    38641 gaagagatgc aggcgtgcgt ggcgcatgga atttatatcg gcattaccgg ttgggtttgc
    38701 gatgaacgac gcggactgga gctgcgggaa ctgttgccgt tgattccggc ggagaaattg
    38761 ctgatcgaaa ctgatgcgcc gtatctgctc ccgcgcgatc tcacgccgaa accatcatcc
    38821 cggcgcaacg agccagccca tctggcccat attttgcaac gtattgcgca ctggcgtgga
    38881 gaagatgccg catggctggc tgccaccacg gatgccaatg tcaaaacact gtttgggatt
    38941 gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt gcttaatctc tttattgata
    39001 agattaagta acagcatgga gcgagcctcg ccgtcgggtt cggtgaaaat ggcctgaaaa
    39061 ccttcgaacg cgccttcggt aataatcacc ttatcacccg gataaggggt tgccggatcg
    39121 acaatgtctt tcggtttata taccgatagc tgatgaatca ccgccgatgg gactatcgct
    39181 ggcgatgcgc caaagcgcac gaagtggctg acaccgcggg tcgcgttgat agtcgtggta
    39241 tgaatcactt ctggatcaaa ttccacaaac aggtagttgg ggaacaatgg ctcactgact
    39301 gcagtacgtt tgccacgcac gattttttcc agggtgatca tcggtgccag gcaattcaca
    39361 gcctgtcttt cgaggtgttc ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa
    39421 taccaggatt gcataatgac tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt
    39481 tacgctaagt taattatatt ccccagtttg cgttataccg tcagagttca cgctaattta
    39541 acaaatttac agcatcgcaa agatgaacgc cgtataatgg gcgcagatta agaggctaca
    39601 atggacgcca tgaaatataa cgatttacgc gacttcttga cgctgcttga acagcagggt
    39661 gagctaaaac gtatcacgct cccggtggat ccgcatttgg aaatcactga aattgctgac
    39721 cgtactttgc gtgccggtgg gcctgcgctg ttgttcgaaa accctaaagg ctactcgatg
    39781 ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg cgatgggcat ggggcaggaa
    39841 gatgtttctg cgctgcgtga agtcggtaaa ttattagcct ttctcaagga acctgaaccg
    39901 ccaaaaggtt ttcgcgacct gtttgataaa ctgccgcagt ttaagcaggt attgaacatg
    39961 ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa tcgtctctgg cgatgacgtc
    40021 gatctcaatc gcattcccat tatgacctgc tggccggaag acgccgcgcc gctgattacc
    40081 tgggggttga ccgtgacgcg tggcccgcat aaagagcggc agaatctggg tatttatcgt
    40141 caacagctaa ttggtaaaaa caaactgatt atgcgctggt tgtcgcatcg cggcggcgcg
    40201 ctggatttcc aggagtggtg tgcggcgcat ccgggcgaac gtttcccggt ttctgtggcg
    40261 ctgggtgctg atcccgccac aattctcggt gcagtcaccc ccgttccaga tacgctttca
    40321 gagtatgcgt ttgccggatt gctgcgcggc accaagaccg aagtggtgaa gtgtatctcc
    40381 aatgaccttg aagtgcccgc cagtgcggag attgtgctgg aagggtatat cgaacaaggc
    40441 gaaactgcgc cggaagggcc gtatggcgac cacaccggtt actataacga agtcgatagt
    40501 ttcccggtat ttactgtgac gcatattacc cagcgtgaag atgcgattta tcattccacc
    40561 tataccgggc gtccgccgga tgaacctgcg gtactgggcg tggcgttgaa cgaagtgttt
    40621 gtgccgattc tgcaaaaaca gttcccggaa attgtcgatt tttacctgcc gccggaaggc
    40681 tgctcttatc gcctggcggt agtgacgatc aaaaaacagt acgccggaca cgcgaagcgc
    40741 gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt acactaaatt tgtgatcgtt
    40801 tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga tttgggcgat tactacccgt
    40861 atggacccgg cgcgggatac tgttctggta gaaaatacgc ctattgatta tctggatttt
    40921 gcctcgcctg tgtccgggct gggttcaaaa atggggctgg atgccacgaa taaatggcca
    40981 ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag atccggatgt tgtcgcgcat
    41041 attgacgcca tctgggatga actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg
    41101 tttgttttgc cctatttatc gatccgacag agaaagcgca tgacaacctt aagctgtaaa
    41161 gtgacctcgg tagaagctat cacggatacc gtataccgtg tccgaatcgt accagacgcg
    41221 gccttttctt ttcgtgctgg tcagtatttg atggtagtga tggatgagcg tgacaaacgt
    41281 ccgttctcaa tggcttcgac gccggatgaa aaagggttta tcgagctgca tattggcgct
    41341 tctgaaatca acctttacgc gaaagcagtc atggaccgca tcctcaaaga tcatcaaatc
    41401 gtggtcgata ttccccacgg agaagcgtgg ctgcgcgatg atgaagaacg tccaatgatt
    41461 ctcatcgcgg gcggcaccgg gttctcttat gcgcgctcga ttttgctgac ggcgctggcg
    41521 cgtaacccaa accgtgatat caccatttac tggggcgggc gtgaagagca gcatctttat
    41581 gatctctccg agcttgaggc gctttcgctt aagcatcctg gtctgcaagt ggtgccggtg
    41641 gttgaacaac cggaagcggg ctggcgtggg cgtactggca ctgtgttaac ggcggtattg
    41701 caggatcacg gtacgctggc agagcatgat atctatattg ccggacgttt tgagatggcg
    41761 aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc gggaagatcg cctgtttggc
    41821 gatgcgtttg catttatctg agatataaaa aacccgcccc tgacaggcgg gaagaacggc
    41881 aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatt ggtgcacgat
    41941 gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgccggatc ggtagaccgg
    42001 ataaggcgtt cacgccgcat tcggtaagtg gttaaatccg ctcaaacacc gtcgcaatac
    42061 cctgacccag accgatacac atcgtcgcca gaccaaactg ggcatctttg cgctccatca
    42121 gattcagcag cgtggtgctg atacgcgcac cggaacaacc cagcggatga cccagcgcga
    42181 tcgcgccacc gttgaggttg atcttctcgt caatctgctc aatcagtccc agatctttaa
    42241 tacatggcag gatctgcgcg gcaaaggctt cattcatttc aaacacgccg atatcactga
    42301 cagaaagccc cgcttttttc agtgccagct tcgaggccgg aaccgggccg taacccataa
    42361 tcgatgggtc acaaccaacg accgccatcg aacgcacgcg ggcgcgcggc ttaagaccta
    42421 attcatgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg gaaagcgcag
    42481 aagatgtacc cgccgttacc gtaccgctga cgggatcaaa cgccggacgc agcgtggcga
    42541 gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc ttcaggacgc
    42601 cgtcggcatc gtgaccaccg gtcgggataa tttcattttt aaatgcaccc gactgcgtgg
    42661 cagcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca cggctgatac
    42721 cgtgcatacg cgccagcatt tctgccgtta agcccatcat gcccgccgct ttggcgacat
    42781 tacggctaag gccggggtga aaatcgacgc cgtgactcat tggcacatgg cccatatgct
    42841 ccacgccgcc aaccagacat gcctgcgcat cgccggtcat gatcattcgt gctgcgtcat
    42901 gcagtgcctg catggatgaa ccacacaagc gattgacagt aaccgccggg acagagtgcg
    42961 gcacttctgc caacagcgcc gcgttacggg cgatattaaa accttgctcc agcgtctgct
    43021 gtacacagcc ccagtaaata tcgtcgaggg cagcagcttc cagcgccggg ttacgcgcca
    43081 gcaggctacg cattaaatga gcggagagat cttccgcacg cacgtgacga aaagcaccgc
    43141 ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt tccattgtga
    43201 ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccggg ggatagtacg
    43261 gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcgtacagt gggccgaggt
    43321 gctgatattg ctgtgccata tcgaggtatt ttgcgctacc gagggtgtcc agccagcgga
    43381 acgcgccacc gtggaacgga gggaaaccca ggccgtagac cagcgccata tccgcttctg
    43441 ccggagtggc gataatgcct tcttccagac agcgcaccac ttcgttgacc atcgggatca
    43501 tcatgcgggc gataatctct tcttcgctga aatcgcgctt cggctggctc acttctgcca
    43561 gcaggtcgtc aacagcggcg tcttcttctt tcttcggctt acctttgctg tcttctttat
    43621 aacgccagaa accgaggccg ttcttctgac caaagcggtt ggcatcaaac agcgcgtcga
    43681 tggcatcgcg ataatctttc tgcatccgct gcgggaagcc tgctgccatg acagcctggg
    43741 catgatgcgc ggtgtcaatg cccacaacgt ccagcagata tgccggaccc atcggccagc
    43801 caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg cgcagcaact
    43861 ggctgaatcc ggcgaagtag gggaacagta cacggttaac gaagaagccg gggcagtcgt
    43921 tgaccacaat cggcgtcttt cccatcttgc tcgcccatgc gacgactttc gcgatggttt
    43981 cgtcggagct tttctcgccg cgaataattt ctaccaacgg catccggtgc accgggttaa
    44041 agaagtgcat cccgcagaag ttttccgggc gttccagcgc gttggccagt tcgctgatag
    44101 gaatggttga agtgttagac gccagcacgg tatccgggcg tactttttgt tcggtttctg
    44161 ccagtacggc ttttttcact ttcgggtttt cgacaaccgc ttctaccaca atatccacgc
    44221 ggtcaaaccc ggcgtagtcg agcgttgggt ggattgtaga gatcacgcca gccagtttca
    44281 gaccatcgat cttgccgcgc tcaagctgct tgttcagcag tttcgcggct tcggtcatgc
    44341 cgagggttaa cgacttgtcg ttgatatctt tcatgacaac cggcacgcct ttccacgcag
    44401 actggtaagc gatgccgccg cccataatgc ctgcgcccag cacagcggcc tgttttgggg
    44461 tttcaacgtc tttggtgagt ttcttcgctt tgccttttac atattgatcg ttaaggaaaa
    44521 tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg gacaaaactt ttgttttcca
    44581 gatttaaggc ttcttcacga ccaaaacggg ccgccgcttc aatggttttt actgcggtga
    44641 tgggggccgg ataatgtttc cccgccgttt gtgcgaccat ccctttagcg atggtgaaac
    44701 tcatggtggc ttcaatcttg ctcagtttta gcggttccag cttcggctga cgtttcgctt
    44761 tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc catcgcccct tcaaccagtt
    44821 tttctgcttt gactacacca tccaccagac cgattttcag cgcctgatcc gcgccgacat
    44881 ctttaccggc ggcaatgatt tccagcgcac tgtcagcgcc cagcatacgt ggcatacgta
    44941 cagaaccgcc aaagcccggc atgatgccca gtttggtttc cggcagaccg atgcgcagat
    45001 ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc gcagccaccg cccagcgcat
    45061 agccgttgac ggcagcaatg gtcggcaccg gcagatcttc caggcgatta aacacgctat
    45121 tggcaaagtg cagccactga cttaactgtt cttcaggaac gaggaacaga gacaaaaatt
    45181 cggtgatatc agcaccgacg ataaaggctg ctttgttcga acgcagcagc agccctttta
    45241 gatctgattg ctgttccagc acgccgatgg cctcgccgag gctggcgacg gtggcagtgt
    45301 cgagtttatt aactgaacct ggggcatcaa ataccagttc ggcaatgcca tcttccagcc
    45361 agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc cgcaaggtga
    45421 tctggtcgta ccagatgcat cgaagtgtgt attttgtgtt aaaaatatgc aaataaaata
    45481 ttaaagaaat gccgatctga tcacgctcgg cagaaatcac gctctggatg aacgatgtgc
    45541 taagatgcgg agacttaagg tcaaaaaaac agaagggtaa aaaatggaat cactggcctc
    45601 actctataaa aatcatatag ctaccttaca agaacggact cgcgatgcgc tggcgcgctt
    45661 caagctggat gcgttactta ttcactccgg cgaactgttc aacgtttttc tcgacgatca
    45721 tccctatccg tttaaagtga atccgcaatt caaagcgtgg gtgccggtaa ctcaggtgcc
    45781 aaactgctgg ctgttggtgg atggcgtgaa caagccgaaa ctgtggtttt atctgccggt
    45841 tgattactgg cacaacgtcg aaccgctgcc gacctccttc tggactgaag atgtagaagt
    45901 gatcgcgctg ccgaaagccg atggcattgg tagcctgctg cctgctgcgc gcgggaatat
    45961 cggttatatc ggtccggtgc cggagcgtgc gctgcaactg ggtattgagg ccagcaatat
    46021 caacccgaaa ggggtgatcg actacctgca ttactaccgc tccttcaaaa ccgagtacga
    46081 actggcctgt atgcgtgaag cgcagaaaat ggcggtcaac ggtcatcgcg cggcagaaga
    46141 agcgttccgt tctggcatga gcgagttcga tatcaatatt gcctatctga ccgcgaccgg
    46201 tcatcgtgat accgacgtac cttacagcaa cattgtggcg cttaacgaac acgctgcggt
    46261 gttgcattac accaaactgg accaccaggc accggaagag atgcgcagct tcctgctgga
    46321 tgccggggcc gaatataacg gctatgcggc tgacctgacc cgtacctggt cggcaaaaag
    46381 cgataacgac tacgcacagc tggtgaaaga cgtaaatgat gaacaactgg cgctgatcgc
    46441 caccatgaaa gcaggcgtca gctatgtgga ttaccacatc cagttccatc agcgcatcgc
    46501 caaattgctg cgtaaacatc aaatcatcac cgatatgagt gaagaagcga tggtcgaaaa
    46561 cgatctcacc ggaccgttta tgccgcacgg tatcggtcat ccgctgggcc tgcaggtgca
    46621 tgacgtagct ggctttatgc aagatgatag cggtacgcac ctcgcggcac cggcaaaata
    46681 tccgtacctg cgctgcaccc gtattctcca gccgggcatg gtgttaacca tcgaaccggg
    46741 tatttacttc attgaatcgc tgctggcacc gtggcgtgaa gggcagttca gcaagcactt
    46801 caactggcag aaaattgaag cactgaaacc gttcggcggc attcgtatcg aagacaacgt
    46861 ggtgatccac gaaaacaacg tggaaaacat gacccgggat ctgaaactgg cgtgatggaa
    46921 agctggttaa ttcctgcggc accagtcacg gtcgttgaag agatcaaaaa gagccgcttc
    46981 ataacgatgt tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt tgaatcggtg
    47041 cgggcagaac accccgatgc ccgccaccat tgcgtggcgt gggtggcggg tgcgccggat
    47101 gattctcaac agctcggttt ctctgacgac ggggaaccgg cgggaacggc cggtaaaccg
    47161 atgctcgccc aactaatggg cagcggcgtc ggggaaatta ccgctgtggt ggtgcgctac
    47221 tacggcggca tattgcttgg taccggtggg ttagtgaaag cgtatggcgg cggcgtgaat
    47281 caggcgctgc gccagctaac gacccaacgc aagacgccat taaccgaata tactttgcaa
    47341 tgtgaatata gtcagttaac cggcattgaa gcgttgctgg ggcagtgtga cggcaaaatt
    47401 atcaacagtg attatcaggc attcgttctg ctgcgggtgg cgcttccggc ggcgaaagtg
    47461 gctgaatttt ccgcaaagct ggcggatttt agccgtggtt cattgcaatt gttagcgatt
    47521 gaagaataat ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg cattttcgcg
    47581 ccattacccg aatcgttgga ctactggtca tcttattttc agggaccatg attatccctg
    47641 ggctggtagc actcatctac cgggatggag cgggccgcgc ttttacccag accttttttg
    47701 tcgccctcgc cattggctct atactgtggt ggccgaaccg caaagagaag ggcgaactga
    47761 aatcccgtga ggggtttctg atagtggtgc tgttctggac cgtgctgggt agcgtcggtg
    47821 cgctcccttt tatcttctcg gaaagcccga acctcacgat taccgatgcg ttttttgaat
    47881 ctttctctgg cctgaccacc acgggggcca ctacgctggt ggggctggat tcgctccctc
    47941 acgccatcct cttttatcgc cagatgctgc aatggtttgg cgggatgggg atcatcgtgt
    48001 tggcggttgc gatactgcct atcctcggcg tgggtgggat gcagctctat cgcgcagaaa
    48061 tgcccggccc actgaaagat aacaaaatgc gcccgcgaat tgcggaaacg gcgaaaaccc
    48121 tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg tttgccggaa
    48181 tggatgcctt tgatgccatc ggccatagct ttgcgactat cgctattggc ggcttctcga
    48241 cacatgatgc cagtatcggt tatttcgaca gcccgactat taacactatc attgctatct
    48301 tcctgctgat ctccggctgt aactacggtc tgcacttttc actgttaagt gggcgtagtc
    48361 tgaaggttta ttggcgcgat ccggaatttc gcatgtttat cggcgtacag tttacgctgg
    48421 tggttatttg tacactcgta ctgtggtttc ataatgtcta cagttcggcg ctgatgacaa
    48481 ttaaccaggc gtttttccag gtggtatcga tggcgacaac cgccgggttt acgactgaca
    48541 gcattgcccg ctggccgctc tttttgccgg tactgctttt atgttcagct tttatcggcg
    48601 gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg catcctgctg ctgtttaagc
    48661 aggggaaccg tgagctgaaa cgactggtgc atccgaacgc cgtctatagc attaagctgg
    48721 ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg gggatttttc tccgcctatg
    48781 cattggtgtt tattgtcagt atgctggcga ttatcgccac gggcgtggat gacttttctg
    48841 cctttgcctc ggttgttgcg acattgaata acctggggcc agggcttggc gtggttgctg
    48901 ataactttac cagtatgaac ccggtggcta aatggatcct gattgccaac atgctgtttg
    48961 gtcgtctcga ggtctttaca ttgctggtgc tctttacccc gactttctgg cgtgaatgat
    49021 ggagtaatac gtgaaaacat taattctttt ctcaacaagg gacggacaaa cgcgcgagat
    49081 tgcctcctac ctggcttcgg aactgaaaga actggggatc caggcggatg tcgccaatgt
    49141 gcaccgcatt gaagaaccac agtgggaaaa ctacgaccgt gtggtcattg gtgcttctat
    49201 tcgctatggt cactaccact ctgcgttcca ggagtttgtc aaaaagcatg cgacgcgact
    49261 gaattcgatg ccgagcgcct tttactccgt aaacctggtg gcgcgtaaac ctgagaagcg
    49321 tactccacag accaacagct acgcgcggaa gtttctgatg agttcacaat ggcgtcccga
    49381 tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt acgaccgttt
    49441 tatgatcaag ctaattatga agatgtcagg cggtgaaacg gatacgcgca aagaagttgt
    49501 ctataccgat tgggagcagg tggcgaattt cgcccgagaa atcgcccatt taaccgacaa
    49561 accgacgctg aaataagcat aaagaa
//
LOCUS       DAYPHN010000014        48848 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000014 DAYPHN010000000
VERSION     DAYPHN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.014"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..639)
                     /locus_tag="ACG0BW_000845"
     CDS             complement(<1..639)
                     /locus_tag="ACG0BW_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257840.1"
                     /translation="MASPINSGTIMSNPCPSTFLDKNRIIAAELNITNNEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFKVIQFDAGEKNTKKIVKETSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KLVNSIVMMNTDCSYEMPADIVSNTYDLLNLRIQKDEGHLPVQEKIDITEDYL"
     gene            complement(904..1194)
                     /gene="ilvN"
                     /locus_tag="ACG0BW_000846"
     CDS             complement(904..1194)
                     /gene="ilvN"
                     /locus_tag="ACG0BW_000846"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFK7257841.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1198..2886)
                     /gene="ilvB"
                     /locus_tag="ACG0BW_000847"
     CDS             complement(1198..2886)
                     /gene="ilvB"
                     /locus_tag="ACG0BW_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFK7257842.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPTAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(2992..3090)
                     /gene="ivbL"
                     /locus_tag="ACG0BW_000848"
     CDS             complement(2992..3090)
                     /gene="ivbL"
                     /locus_tag="ACG0BW_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFK7257843.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3655..3744
                     /gene="tisB"
                     /locus_tag="ACG0BW_000849"
     CDS             3655..3744
                     /gene="tisB"
                     /locus_tag="ACG0BW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFK7257844.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(3868..3942)
                     /gene="ysdE"
                     /locus_tag="ACG0BW_000850"
     CDS             complement(3868..3942)
                     /gene="ysdE"
                     /locus_tag="ACG0BW_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFK7257845.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            4024..5208
                     /gene="emrD"
                     /locus_tag="ACG0BW_000851"
     CDS             4024..5208
                     /gene="emrD"
                     /locus_tag="ACG0BW_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFK7257846.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(5216..5713)
                     /gene="yidF"
                     /locus_tag="ACG0BW_000852"
     CDS             complement(5216..5713)
                     /gene="yidF"
                     /locus_tag="ACG0BW_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFK7257847.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5710..6072)
                     /gene="yidG"
                     /locus_tag="ACG0BW_000853"
     CDS             complement(5710..6072)
                     /gene="yidG"
                     /locus_tag="ACG0BW_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFK7257848.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(6062..6409)
                     /gene="yidH"
                     /locus_tag="ACG0BW_000854"
     CDS             complement(6062..6409)
                     /gene="yidH"
                     /locus_tag="ACG0BW_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFK7257849.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            6517..6966
                     /gene="yidI"
                     /locus_tag="ACG0BW_000855"
     CDS             6517..6966
                     /gene="yidI"
                     /locus_tag="ACG0BW_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFK7257850.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILNQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(7013..8506)
                     /gene="yidJ"
                     /locus_tag="ACG0BW_000856"
     CDS             complement(7013..8506)
                     /gene="yidJ"
                     /locus_tag="ACG0BW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFK7257851.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDGTF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDGGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(8503..10218)
                     /gene="yidK"
                     /locus_tag="ACG0BW_000857"
     CDS             complement(8503..10218)
                     /gene="yidK"
                     /locus_tag="ACG0BW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFK7257852.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIHNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            10385..11278
                     /gene="yidL"
                     /locus_tag="ACG0BW_000858"
     CDS             10385..11278
                     /gene="yidL"
                     /locus_tag="ACG0BW_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFK7257853.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKIILKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYSDGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLNARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(11275..12597)
                     /locus_tag="ACG0BW_000859"
     CDS             complement(11275..12597)
                     /locus_tag="ACG0BW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFK7257854.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAENSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSAAVAKAILDELLEANKAYWPELR"
     gene            complement(12597..14213)
                     /locus_tag="ACG0BW_000860"
     CDS             complement(12597..14213)
                     /locus_tag="ACG0BW_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFK7257855.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            14509..15225
                     /gene="yidP"
                     /locus_tag="ACG0BW_000861"
     CDS             14509..15225
                     /gene="yidP"
                     /locus_tag="ACG0BW_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFK7257856.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(15222..16883)
                     /gene="yidE"
                     /locus_tag="ACG0BW_000862"
     CDS             complement(15222..16883)
                     /gene="yidE"
                     /locus_tag="ACG0BW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFK7257857.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(17079..17507)
                     /gene="ibpB"
                     /locus_tag="ACG0BW_000863"
     CDS             complement(17079..17507)
                     /gene="ibpB"
                     /locus_tag="ACG0BW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFK7257858.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(17619..18032)
                     /gene="ibpA"
                     /locus_tag="ACG0BW_000864"
     CDS             complement(17619..18032)
                     /gene="ibpA"
                     /locus_tag="ACG0BW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFK7257859.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(18026..18098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            18338..18670
                     /gene="yidQ"
                     /locus_tag="ACG0BW_000865"
     CDS             18338..18670
                     /gene="yidQ"
                     /locus_tag="ACG0BW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFK7257860.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(18672..19886)
                     /gene="yidR"
                     /locus_tag="ACG0BW_000866"
     CDS             complement(18672..19886)
                     /gene="yidR"
                     /locus_tag="ACG0BW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFK7257861.1"
                     /translation="MKQITFAPRNHLLTNTNIWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGARVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GIAAPFGPVNIQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETER"
     gene            19987..21087
                     /gene="cbrA"
                     /locus_tag="ACG0BW_000867"
     CDS             19987..21087
                     /gene="cbrA"
                     /locus_tag="ACG0BW_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFK7257862.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAALLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHISARYLVGADGANSM
                     VRRHLYPNHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTALKEKMSAFQFQFGEAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(21048..22385)
                     /gene="dgoT"
                     /locus_tag="ACG0BW_000868"
     CDS             complement(21048..22385)
                     /gene="dgoT"
                     /locus_tag="ACG0BW_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7257863.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(22460..23608)
                     /gene="dgoD"
                     /locus_tag="ACG0BW_000869"
     CDS             complement(22460..23608)
                     /gene="dgoD"
                     /locus_tag="ACG0BW_000869"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFK7257864.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(23605..24222)
                     /gene="dgoA"
                     /locus_tag="ACG0BW_000870"
     CDS             complement(23605..24222)
                     /gene="dgoA"
                     /locus_tag="ACG0BW_000870"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFK7257865.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSIERTAQQAAAFVKAYREAVQ"
     gene            complement(24206..25084)
                     /gene="dgoK"
                     /locus_tag="ACG0BW_000871"
     CDS             complement(24206..25084)
                     /gene="dgoK"
                     /locus_tag="ACG0BW_000871"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFK7257866.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWHEENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVATVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(25081..25770)
                     /gene="dgoR"
                     /locus_tag="ACG0BW_000872"
     CDS             complement(25081..25770)
                     /gene="dgoR"
                     /locus_tag="ACG0BW_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFK7257867.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            26048..26704
                     /locus_tag="ACG0BW_000873"
     CDS             26048..26704
                     /locus_tag="ACG0BW_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257868.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGGEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKKFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(26748..27560)
                     /gene="yidA"
                     /locus_tag="ACG0BW_000874"
     CDS             complement(26748..27560)
                     /gene="yidA"
                     /locus_tag="ACG0BW_000874"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFK7257869.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(27675..28073)
                     /gene="yidB"
                     /locus_tag="ACG0BW_000875"
     CDS             complement(27675..28073)
                     /gene="yidB"
                     /locus_tag="ACG0BW_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFK7257870.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGSNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(28313..30727)
                     /gene="gyrB"
                     /locus_tag="ACG0BW_000876"
     CDS             complement(28313..30727)
                     /gene="gyrB"
                     /locus_tag="ACG0BW_000876"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFK7257871.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEDDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(30756..31829)
                     /gene="recF"
                     /locus_tag="ACG0BW_000877"
     CDS             complement(30756..31829)
                     /gene="recF"
                     /locus_tag="ACG0BW_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFK7257872.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(31829..32929)
                     /gene="dnaN"
                     /locus_tag="ACG0BW_000878"
     CDS             complement(31829..32929)
                     /gene="dnaN"
                     /locus_tag="ACG0BW_000878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFK7257873.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(32934..34337)
                     /gene="dnaA"
                     /locus_tag="ACG0BW_000879"
     CDS             complement(32934..34337)
                     /gene="dnaA"
                     /locus_tag="ACG0BW_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFK7257874.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            34634..34714
                     /locus_tag="ACG0BW_000880"
     CDS             34634..34714
                     /locus_tag="ACG0BW_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257875.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            34944..35084
                     /gene="rpmH"
                     /locus_tag="ACG0BW_000881"
     CDS             34944..35084
                     /gene="rpmH"
                     /locus_tag="ACG0BW_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFK7257876.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            35101..35460
                     /gene="rnpA"
                     /locus_tag="ACG0BW_000882"
     CDS             35101..35460
                     /gene="rnpA"
                     /locus_tag="ACG0BW_000882"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFK7257877.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            35424..35681
                     /gene="yidD"
                     /locus_tag="ACG0BW_000883"
     CDS             35424..35681
                     /gene="yidD"
                     /locus_tag="ACG0BW_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFK7257878.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            35684..37330
                     /gene="yidC"
                     /locus_tag="ACG0BW_000884"
     CDS             35684..37330
                     /gene="yidC"
                     /locus_tag="ACG0BW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFK7257879.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            37436..38800
                     /gene="mnmE"
                     /locus_tag="ACG0BW_000885"
     CDS             37436..38800
                     /gene="mnmE"
                     /locus_tag="ACG0BW_000885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFK7257880.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPD
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            39042..39116
                     /gene="tnaC"
                     /locus_tag="ACG0BW_000886"
     CDS             39042..39116
                     /gene="tnaC"
                     /locus_tag="ACG0BW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFK7257881.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            39337..40752
                     /gene="tnaA"
                     /locus_tag="ACG0BW_000887"
     CDS             39337..40752
                     /gene="tnaA"
                     /locus_tag="ACG0BW_000887"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFK7257882.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            40843..42090
                     /gene="tnaB"
                     /locus_tag="ACG0BW_000888"
     CDS             40843..42090
                     /gene="tnaB"
                     /locus_tag="ACG0BW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFK7257883.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYSPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            42223..43398
                     /gene="mdtL"
                     /locus_tag="ACG0BW_000889"
     CDS             42223..43398
                     /gene="mdtL"
                     /locus_tag="ACG0BW_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFK7257884.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTNSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            43373..44332
                     /gene="yidZ"
                     /locus_tag="ACG0BW_000890"
     CDS             43373..44332
                     /gene="yidZ"
                     /locus_tag="ACG0BW_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFK7257885.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKHLYASMA"
     gene            44489..45238
                     /gene="yieE"
                     /locus_tag="ACG0BW_000891"
     CDS             44489..45238
                     /gene="yieE"
                     /locus_tag="ACG0BW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFK7257886.1"
                     /translation="MATHFARGILTEGHLISVRLPSHCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            45260..45826
                     /gene="yieF"
                     /locus_tag="ACG0BW_000892"
                     /gene_synonym="chrR"
     CDS             45260..45826
                     /gene="yieF"
                     /locus_tag="ACG0BW_000892"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFK7257887.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(45880..47217)
                     /gene="adeP"
                     /locus_tag="ACG0BW_000893"
     CDS             complement(45880..47217)
                     /gene="adeP"
                     /locus_tag="ACG0BW_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFK7257888.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            47384..48049
                     /gene="yieH"
                     /locus_tag="ACG0BW_000894"
     CDS             47384..48049
                     /gene="yieH"
                     /locus_tag="ACG0BW_000894"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFK7257889.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVELEEIFT
                     RFKGVKLYEIIDIISKEQGVTMIQADAEHVYRAEVARLFDTELEVIAGANELLASINV
                     PMCVVSNGPVKKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWK
                     ITR"
     gene            complement(48186..>48848)
                     /locus_tag="ACG0BW_000895"
     CDS             complement(48186..>48848)
                     /locus_tag="ACG0BW_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFK7257890.1"
                     /translation="RIKFVESIINKMIPPPDGTILTENPKEYVQKQIKESHKESATNV
                     TFDYKELAPIFEGVQKKQIEALSNQLEHIKSFKTDYDSRLNKFARDFQYLSSAFSINR
                     QDLLKNYQEIRASITDYDDLLREIKELTISRDKSVDDKRTLFDKKRDNWNSQEVQDEV
                     KALNEDISDSDNKIRSKLNIVQNHRRENQYKANKNISNAMLNILDWFERYPDIVKNIN
                     QA"
BASE COUNT        11761 a        12811 c        12340 g        11936 t
ORIGIN      
        1 taaataatcc tcagtaatat caattttttc ctgaacaggt aaatggccct catctttttg
       61 aattcgcagg tttaataaat catatgtatt actcacaatg tctgcaggca tttcgtaact
      121 acaatccgta ttcatcataa caatactatt aactaatttt ttcctcaaaa ccgtgtcagc
      181 agaatgtgtt tctaacgcag cagttttttc agacagcgat ttgataatag tcgccacagc
      241 cactccagaa tatgatgttt cttttactat tttttttgta tttttttcac ctgcatcaaa
      301 ctgtataact ttaaaggatt tgccacctgt ttgttcgatt acggtatatt cgttctctcc
      361 agttgccgcc atccaggtac gattaccatt attttcagtg cctggcatag ggtgaaaata
      421 gttaaggatt tcggtattaa cactgcgaga accggacata atatttgcta ttaagcgttt
      481 tatttcctgt aacatccatt ttgcgaaatt actgccaggt gaatattttt tctcgttatt
      541 tgtgatatta agctcagcag caataattct atttttatcc aaaaaggtgg aggggcaggg
      601 atttgacata attgttccgc tgtttatagg gcttgccata atatacctca cgatgaacca
      661 ttaattaata gaatgctggt catgaccact ggtatagaca gcaatcagaa tttaacacca
      721 tcgctggaaa tttttagccc taactgtcaa ccccctggtt atcggtgcag cgacttaaat
      781 taacgtcctg ggctaataaa ttgtgcctga gatggtctct ggcacaattt caccctatat
      841 atctgaacgg atctatcaaa aatcgctcat cagtaacccc cccgagcggt ttaatattgc
      901 tccttactga aaaaacaccg caatcttgtt aaacattgtc ggatcggact gattacgctg
      961 cactttcacc acatcttcca gcttatcgat ttggcttatc atctgctcca gacgctggtc
     1021 gtcattgacc agtagccaga tatggctttt gtcgctgtcc tgaatcggca ggcagagaat
     1081 gccttcgacg ttaaaagcgc gacgggcaaa aaggccacaa acgtgggtca ttacgcccgg
     1141 atggttgcga acggtgagtt cgagaatcac gttgtcatga gttgtgtttt gcatggctta
     1201 ttcccccacc atttcagtat tcgccgcacc tggcggcacc atcgggtaca ctttttcttc
     1261 ggcatcaatg cgcacatgga tcagtgccgg accagggcga ttgatgattt cctgcaatgc
     1321 agcctgcgga tcggcttcgt tattcaaatc acaggtttcg aggccgaatc cggcggcaat
     1381 ttgcataaag ttgattttgc ccggataggt ggcagcaaaa acgccctgct tgtagaacag
     1441 actctgttgc tgatgcacca gccccagcgc ttcgttgttc atcagaatga ttttgacatc
     1501 cagctggttt tcgctggcag tcgccatctc ctgaatattc atcatcaggc tgccgtcgcc
     1561 ggagaagcac aacactttgc gatccgggtt cgccagcgcc gcgccaatcg ccgtaggcag
     1621 gccaaaaccc atcgtgccca gcccaccgga ggtcagccac tggcgtgggc gattgagcgg
     1681 ataagcttgc gcggtccaca tctgatgctg acccacgtcg gtggtgataa tcgcgctgtc
     1741 atcgacacag gcggcaacgg cgttgatcag gccgtaatgg cttaacggat cgcacgcttt
     1801 cgggattgga cacgggaatt cgcgctgcaa atccgctacc aactggtgcc actctgcacg
     1861 cggttgcgct tccaccagcg ggatcaactg cgccagcacg tcatcaacat ccgcctggat
     1921 cgccacatgt ggctgcttga ttttacccag ctctgcgcgg tcgatatcga catgaataat
     1981 tttggcattc gggcagaact gctcggtttt gccaatcgcc cggtcatcaa aacgcgcgcc
     2041 gagaacaatc agcagatccg cctcctgcaa gatatagttg gtgctgcgca cgccgtgcat
     2101 tcccagcata cccagcgaca acggatgcgc ttttggcaac atgcccagcg ccattaaagt
     2161 catggtggta ggcagctgcg ctttctccgc cagttcacgc actcgcgcag gcgcattgat
     2221 cacaccaccg cccagataaa gcaccgggcg tttggcagca ttaatcattg ccgctgcgtc
     2281 acgaatgctt tcttcgctaa aggcgggcgc gacggctttt tctgccaccg cgggctgtgc
     2341 ttcaatctca aaaactgccg tttgcacatc cttaggaatg tctatccaca ccgggccagg
     2401 gcggcctgat tgcgcaatgc ggaaggcatc gctcatgacc tgcgggagtt cttcgatatg
     2461 tctgaccaga tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt ccacttcctg
     2521 gaaggcgtcg gtgccaatca tcgaggcagg aacctgaccg gtgatgcaaa tcagcgggat
     2581 ggaatccagc cgcgcatcgg caatggcggt caccaggtta gtcgcacccg gtccgctaca
     2641 ggccatacag accgccggtt taccgtcggt gcgcgccatt ccctgagcga taaaccccgc
     2701 gccctgttca tggcgagcca gaatatggcg gatttgcgtg ctttggctta aggcatcgta
     2761 aacaggcagg atagaaccgc ccggaatgcc cgtcacaatc ttaatgccct gctgttccag
     2821 gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc gtcgatgttg tgcccgaact
     2881 tgccatgctc cagtcctttt cttctgggcc gactttccgg gaggtgctta aacgaaaaac
     2941 cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt tgttccagtc cctacggcgc
     3001 attgccgacg accaccacca cacgcacgac gaccactgcg gcggatggcg cagttggtag
     3061 tagttttgcg ttgaacatgg aagtagtcat tggggacctt gttggttttg tgtttaacaa
     3121 tatttataca agcacagctt tacaggggag acaatggaaa atttttcagc aagggaaaat
     3181 tgaggggttg atcacgtttt gtactgaatt gcaggtaaca aaaaaccccg ccggagcgag
     3241 gtttcgtcag tcgcctgcgg ctggtaaccg caaagcacac tgtattatgt caacactgaa
     3301 agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga attttgacgt atttagtgca
     3361 tagttgagta tcgatcacag tttgcgtttt gtccaaatat tactgtttat ttatacagta
     3421 aacttctata atatcactgt acgcaatgtg ttatgtgggg gccgcatcgt tacccggcgc
     3481 actaagtcct ggctgaaacg ggtggtgccg tcagcgcctt aaccccgcgt gagcacactg
     3541 tgttatgtca acgagcacaa cgtttctcct tgagataccg cgtgcacaac agctggcaac
     3601 aggcagcgga aaggtacgtc agctggcagt gctcctgaac cacaggagac gcgtatgagc
     3661 ctggtggata tcgccattct tatcctcaaa ctcattgttg cagcactgca actgcttgat
     3721 gctgttctga aatacctgaa gtaattcaga ttcaagtcgc actaaagggg agcgggtaac
     3781 cgctcccctt ttatatttag cgtgcgggtt ggtgtcggat gcgatgctga tgcatcttat
     3841 ccgccctacc atccctcccg gcaacattta ttgccgcttt tgtttacata ttctgccgct
     3901 aaacaattcc ccattcctgg cgtatatctg gctaacattt atcaatgtga tagattcctc
     3961 tcccgcattt atgggaatgc gtagtgactt attctaatta tttttataaa agcatccgtg
     4021 ataatgaaaa ggcatagaaa cgtcaatttg ttattgatgt tggtattact cgtggccgtc
     4081 ggtcagatgg cgcaaaccat ttatattcca gctattgccg atatggcgcg cgatctcaac
     4141 gtccgtgaag gggcggtgca gagcgtaatg ggcgcttatc tgctgactta cggtgtctca
     4201 cagctgtttt atggcccaat ttccgaccgt gtgggtcgcc gaccggtgat cctcgtcgga
     4261 atgtccattt ttatgctggc aacgctggtc gcggtcacga cctccagttt gacagtattg
     4321 attgccgcca gcgcgatgca ggggatgggc accggcgttg gcggcgtaat ggcgcgtact
     4381 ttgccgcgtg atttatatga acggacacag ttgcgccatg ctaatagcct gttaaacatg
     4441 ggaattcttg tcagtccgtt gctcgcaccg ctaatcggcg gtctgctgga tacgatgtgg
     4501 aactggcgcg cctgttatct ctttttgttg gtactttgtg ccggtgtgac cttcagtatg
     4561 gcccgctgga tgccagaaac gcgtccggtc gacgcaccgc gcacacgcct gcttaccagt
     4621 tataaaacgc ttttcggtaa cagcggtttt aactgttatt tgctgatgct gattggcggt
     4681 ctggccggga ttgccgcctt tgaagcctgc tccggcgtgc tgatgggcgc ggtgttaggg
     4741 ctgagcagta tgacggtcag tattttgttt attctgccga ttccggcggc attttttggg
     4801 gcatggtttg ccggacgtcc taataaacgc ttctcaacgt taatgtggca gtcggttatc
     4861 tgctgcctgc tggctggctt gctgatgtgg atccccgact ggtttggcgt gatgaatgtc
     4921 tggacgctgc tcgttcccgc cgcgctgttc tttttcggtg ccgggatgct gtttccgctg
     4981 gcgaccagcg gcgcgatgga accgttcccc ttcctggcgg gcacggctgg cgcgctggtc
     5041 ggcggtctgc aaaacattgg ttccggcgtg ctggcgtcgc tctctgcgat gttgccgcaa
     5101 accggtcagg gcagcctggg gttgttgatg accttaatgg gattgttgat cgtgctgtgc
     5161 tggctaccgc tggcgacgcg gatgtcgcat caggggcagc ccgtttaagc tcacgtcacc
     5221 gcagcatcgt catcagctcc atgggagaac gatgctgctt tatcagatca cgcatcaccc
     5281 gcatatgcgg tgcggagtaa gaataaaacg cctgatagcc cgcacatagc acgctgtctg
     5341 gcgtctcctc ccgagcgtgc gggcacagcc gatgccagtc gcacgcctgg cagccacggc
     5401 gcggttgcgg gttttcgctg ccacaccatt tttgtaatgt ggcgtcaaaa agctcaatcg
     5461 agatacgact gatatctgcc cgcacccaac actcaaacag ggcaattaaa aatttccccc
     5521 acagctctgc tgtcaccgaa gcggccgtca gatcgccccg gtcatcaaat tcaagatggg
     5581 gaatgagctg tacggagcgg ataccccgtt gtttgaaatt gtgataaaaa tcggcaggtt
     5641 gcagagcatc cttgtactct acaaccagtt tatgcctgtc ctcttcagca tcaatgacct
     5701 gacttcctgt catgcgactc tctcaataaa tacgataagt tgatgtatat gcgttacagc
     5761 aaacagtatt gcgagagata acaccgcgag ggagatcaaa aatttgtcac ggactacgtg
     5821 aaattgggaa aaatcgctat tcgtgacatc cattaaattg cgattacggg tgtagtgcca
     5881 gaggatcagc gccacgatgg cgagaatgcc aatcgaaatc cagaataaca tgcccgcctg
     5941 gtgccagttg tgtttgatag ccaacgccat caacgcgccg tagcccagca tggtacgaaa
     6001 ccacgccagt gatgtgcgct ccggctgcag tcctgggtcg gcaatgcgtc tggctttgcg
     6061 gctatccggc atacaacacc agtcccataa cgatcaccgc cacgaccatc aatattaagc
     6121 taatgattaa taagctgttg gtgtagggca ggtcctcttt cagacgcatc gctttttcat
     6181 tgcgtaacca gcgcaagtag ccatacatcg ctaacccgcc ggaaaacaga cacaacaaca
     6241 acgccagcag ttcgcgaatg acgggcgtgg cgaaatctgg cgcaagttga tcaagcccaa
     6301 cccccgccgc cagaaatcct agtgcagtac ggatccacgc cagaaaagta cgctcattag
     6361 ccagcgagaa gcggtaatca ggcgcttctc cgaggcggga aattttcatt gttgctcctt
     6421 tgcaatttca ctaacgctct acctttagcg gtttttttaa gaatttcgtc cgcccgtttc
     6481 ctgatatgct tttgccatcc gctacaagga ggtcagatgg gtatcatagc gcaaaataaa
     6541 atcagttcat tgggcatgct gttcggtgcc atcgctttaa tgatggggat tatccacttt
     6601 tcattcggcc ctttctccgc accaccgcca acgtttgaaa gcatcgtggc ggacaaaacg
     6661 gctgaaatta aaagaggtct tctcgccgga ataaaaggcg aaaaaattac caccgtggag
     6721 aaaaaagagg acgtagatgt cgataaaatc ctcaatcaaa gcggtatcgc actggccatt
     6781 gctgcgctac tttgcgcatt tattggcggt atgcgtaagg aaaatcgctg gggcattcgc
     6841 ggcgcgctgg tttttggcgg cgggacgctg gctttccata ccttgctttt tggtatcggc
     6901 atcgtgtgca gcattttgct gatattcctt atcttttcct tcctgaccgg cggttcactg
     6961 gtttaagcag cctgaataat gcccgccggg tgatcatcag cgggcattat cgtcagaact
     7021 tctgtttttt ctcctccacc ttcacccctt gtgtcggtag gccggtgtca tagtcgcgta
     7081 caactggcga atagccatct tgtggacgtg gacgaaacgc ccccatccag cgcggccgtg
     7141 catctttacg ccacggacgc agactccatt ggtaactgcg gaacggatcg cgaattttgt
     7201 ccatgtaatc caataaggcg tcatgcattt tgctgcgaac gtctgcaaaa cggatatcat
     7261 cgatcaggtt atgcatttca tttgggtcat tacggcgatc gtaaagttca tcactggtga
     7321 agaggttgag taccagttta aagtcatccg tcacccagca acgcaccgga ataaaaccgc
     7381 caaagctgtc atgctcaatc tcgtagcggt taaattccac catcacgccg cgtggctctt
     7441 tcacggcaag gatattttcc cccggcagaa tctctggttt ttcaatatct gccagcgcca
     7501 tcattgtcgg cagtaaatcg atatgactga ctggcgtatc gacctgtcgc cgctcccctt
     7561 gcggcgaacg gatgatcagc ggaatgcggg tgatgtcgtc atacatcgcc gctcctttac
     7621 tgatcagctt atgtgcgccc atcatttcgc cgtgatcgga tgtataaata acccacgtat
     7681 tttcacgttg ctctggcgtt aaggcattga tgacccgtcc gatttggtca tcaacaaagt
     7741 cattgcaggc aaaatagagc ggatggtgat aaagcccgcc atcaccgact ggcgatggca
     7801 tcgcctgcgc ccataagcgg tgatgttccg gtttgttagc caggtcatcc tgtgctttct
     7861 cgcccagctc gtagtaaaaa tcagcgtatt tctctaaata ctccaccgga caggtgaacg
     7921 ggtgatgcgg ctcatcatac gaaaccacca tcaggaaggg ttcctcggcg cgcgcgggct
     7981 gttgcagaaa atctaccgcc cgattgctga tgcggtgcgc ccaggtgaag gttccgtcga
     8041 tatggttcgc ctgtaaatcc tcaacgctgt ttaggccatt gcgccacagg ctgatctctt
     8101 tttccgtcag ttcgctaagg tagttcgccc catcgaacca gtaatcagcg tcccactccg
     8161 gcggacactc gccagtgccg aaatagtcat gaccgtcgag atgccatttg ccgatgtaac
     8221 aggtgtgata gccggcatcc ttaaagtagc gccccatagt ggagatgttt ttgcctggcg
     8281 cgacgttgtt ggtccacggg ccggactggt tagcgtagat accggtaaat agtccggcgc
     8341 gtgcaggcgt acaaaccggt gaacaggtgt aggcggaatt aaagcgaata ccttccgccg
     8401 ccagactatc aatattttgc gtattcagcg gtttaccgct atagcaaccg accatattgg
     8461 tggcctgggt atcggtcatg atgaacagaa aattggggcg tttcataggc tatccttcgc
     8521 gtcgggagta aaggttacgg ctgggtcgtg acgatgccgc cagctgtcaa aaatcaggta
     8581 gacaatcact acggcggcaa tgaaataact gatcatcgct aaccagcgcg taccgtagcc
     8641 gccgaattca gccagcccgg catagacgcc aatcatcgcg aacaggatgc caattgacgc
     8701 gatcttgacg tttctccacg gtttcatgtc caccgcaaac gcatctttga aggtgaacgg
     8761 cgtggcgcgc ggtttgataa aaccgatcac cagcatcacg accacgttga tgcagaacgt
     8821 acaggccagc acatagagga aatggaagtc gaacttcacc agatagttga tggtgatgta
     8881 gctgattatg ccaatcccca tcgccacttt tgccgccagc gccgggatgc gcgggaagaa
     8941 aaagcccata atgatgatgg taaccagcgg cacgttgtga atgccgttga gctgtttcat
     9001 ccagctatac agcccctgcg gcgcgttggc gatccacggc gcgaccagca ccgaaacgat
     9061 agcgataaag aaaccaaatt tgcgcccgac ggtgaccagc tgctgcggct cggcattctg
     9121 gttaatgatg cgacggtaaa tacccatact gaataacgta ctggcgctat tcagaaagcc
     9181 gttgaaggta ctgatcaccg caccaaataa caccgcgccg aagaaaccca ccattggcac
     9241 tggcagaacg ttattgacca gcgtcgggta ggccatgtcg gctttcggta aatcctgata
     9301 cagatgaaac gcaatcaacc ctggcagcac cagtaccagc gggtcgagca ttttcagcac
     9361 cgccgttaac agcgccccct tttgcccttc cgccaggctt tttgacgcca gcgtgcgttg
     9421 cacgatgccc tgatttgtac accagtaaaa ggtgttcacc agaatcaaac cggtaaatgc
     9481 cgcgccaatc ggcaagggat cggtcgggcc accgattgag tttaatttct cggcgtgaac
     9541 ggtggtgagt tgctcaatgc cctgcataaa gctgcccttg cccatcgcga tcaggccaaa
     9601 taccggcacc atcaacccgc cgataaccag cccaataccg ttgatggagt ccgccactgc
     9661 cattgcgcgc agtccgccga tcaccgcata caaaattccc gccagaccaa gcaaaattac
     9721 cagtagccag atagccgcac cgtgagaaat ctgtagcgat tccccgacgt gaaacaggct
     9781 gttgagcgcc aacgcgccgg agtagagaac aatcggcaga aagcagacgc cggtggcaat
     9841 gaggaagcag aagtcgatga taatacgcgt cgttttatca taacgttcct ccagaaaatc
     9901 ggggatggtg gcaatgccgc gcttcagata gcgcggtaaa aagattagcg cgaggaagat
     9961 cagcgtcacc gctgaagtca cttcccagcc catcaccgac atgccgcttt tgtaggcctg
    10021 cccggaaagg ccgaccagtt gttccgtgga aaggttggtt agcattaacg aagcggcaat
    10081 aaccggcgct tttagtgaac gtccggcaag aaaatagcct tgttgcgatc cggtatctgt
    10141 tttgcggacc ttccaccagg tgatcaccgc caccagcagc gtaaaaccga caaaactcaa
    10201 gatttgtaac gaattcatct catagccctt atttatgttt atgtcccgga cgcatgaata
    10261 tagggagagg taaaatgcgt cgggtcaggc agtcagattc ttaaaataca aacatcttat
    10321 ccctgaacgg attgttttct gttaagttca ggttgtgagc ataattcgcc gaagggatat
    10381 taaaatgaat ggaaaattgc aaagttcgga tgtaaaaaac gaaactccgt acaatattcc
    10441 cttattgatt aacgaaaatg tgatctccag cggaatttct ctgatctcgc tctggcatac
    10501 ctacgccgac gagcattacc gggtgatctg gccgcgggac aagaagaaac cgcttatcgc
    10561 caactcatgg gttgcggtgt ataccgtaca aggatgtggg aaaattattt taaagaatgg
    10621 tgaacaaata acactgcatg gcaactgtat tatattttta aagccaatgg atattcactc
    10681 ttatcactgt gaaggtttag tctgggaaca gtactggatg gaatttaccc ccaccagtat
    10741 gatggatatt cccgttggtc agcaaagcgt tatttataat ggcgaagttt ataatcagga
    10801 actcaccgaa gttgctgaat taattacttc accagaagca ataaaaaata atctggcagt
    10861 cgcttttctg acgaaaatta tttatcagtg gatttgtctt atgtactcag acggtaaaaa
    10921 agatccacaa cggcggcaaa ttgaaaaact aattgccact ttacatgcca gtctgcaaca
    10981 acgctggagc gtagctgata tggctgccac gatcccctgt agcgaagcct ggttgcgtcg
    11041 tctgttttta cgctataccg gcaagacgcc gaaagaatat tacctcaatg cacgtctgga
    11101 tctggcgcta tcgctattaa agcaacaagg gaactcggtt ggcgaagtcg ctgatacgct
    11161 caacttcttc gactcctttc atttcagcaa agcctttaaa cataaatttg gttatgcgcc
    11221 gtcagctgtg ctgaagaata cggaccagca cccaacggat gccagtccac acaattaacg
    11281 taactctggc cagtacgctt tgttggcctc cagcaactca tccagaatag ctttggcgac
    11341 cgcggcgctc ggtaccgttt tcgacaacgt aatcgcctgc cacaggtgct gatatgaacg
    11401 ctgctcccag gcgtccacga ccagtttttc caccgccact tgctgactca ttaacccttt
    11461 ctgaaactgc gggatatcgc cgaccaccag tggttctggt ccgttgtgcc cgaccagaca
    11521 cgggatctcg accatcgctt catcatcaaa gttatggatt gccccgttgt taggcacaat
    11581 cagcaacatc cgctcctgag tgttgaaggc aattgccgcc gccagatcga cgatatatga
    11641 cgcatgttcg tcaatttcca gcttgcccgc cgctgaattt tctgccgccg taatggcgcg
    11701 gcaagcatcg aaaacctgtt tctcgcgatg ttccatcacc tcattcgcgc gggtatgttc
    11761 agggttggaa tgctgaacga cataatccgg gaagagataa tatttcagat aggtgtttgg
    11821 taatgtatcc gggtccagtg cctggacatc ccgcgctttt gcataggtgt cgttccagct
    11881 cgcttcagta tgtgtatctt gctgcggtgg aacataaccg tgcttagaga catattgccg
    11941 cagctggggc atcaggtcgt tgccctgcaa atcttcaatt gatgtccacc agccaaagtg
    12001 gttcaggccg tagtagcgca cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc
    12061 catccggctt tcaataccga ttggcatgtc acagatgttg aggattttcg cattcgggcg
    12121 cagacgacgc gtggcttctg cgacaatagc tgccgggttg gagtagttga gcatccaggc
    12181 atttggtgaa tatttttcca tataatccac cagttccagg acgccgccaa tggaacgcat
    12241 gccgtacgct attccgccgg gtccgcaagt ttcctgacca acaacgccgt ggcgcagcgg
    12301 gattttttca tccagttcgc gcatcgggta tttgccgacg cggatgtgcg ccatgacaaa
    12361 atcaacgtcg ctgaatgcca cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc
    12421 tttttctttt aggatgactt tacaggcttc ggcaatcact tcctgccgcg cgccatcgtt
    12481 atcataaaat ttcagtgcac gaagcgggaa acggtcctga ttcgccagga gcatcaacac
    12541 gatgcctggc gtaaaggtgc ttccaccgcc tgcgacaacc actgagaatt tggtcattat
    12601 actgcctccg taatggcaac attttctgct gattgatgag aattaattaa gctatcgagc
    12661 tgttcacgca gctgggatac atgcagacca atgatcacct gaatggcatc gccactacgg
    12721 aagacgccgt gcgctcccag ctttttaaag acttcgtcat ccagcgtttg tgacatgtca
    12781 tgcagtgcaa tccgtagacg cgtcgcgcaa ttgttgatgc tggagatatt gccgaccccg
    12841 cccagggctt gcaggatacc ggcagcctga tccagctctt tttttggctc tgccgcggtg
    12901 gtttggcctc gcgaggcttt gtattcggct tttgagtaga gtttcacttc cgcatcttca
    12961 cgtcccggcg tgcacatgtt gaattgcaga atcagggtgc ggaaaaccac gaagtacagc
    13021 agggtaaagc acaacccaat ggcgatttgg gtcagcatca tatccgcgtg gttgctgaac
    13081 atcgggatcc agttttgcgg taaaacctgg tcaatcaaac caccgcccat gttgcccacc
    13141 acaccaaaga gatacattac ggtcgacatt gaggccgcca gcacggcgtg taccgcaaac
    13201 agcaacggtg aaatgaacag gaaggtaaat tccagcggtt cggtaattcc caccagcatg
    13261 gcggttaagg ttgcggggat cagcaagccc gccactttta cccgattttc cggtgcggca
    13321 gtgaagtaca tcgctaaaga aatgcccacg gcaccaaaga tttttgagtt accgtgcagg
    13381 gcaaaaccgc cttccgggaa caacgatttc agcggctcgg cgctcaaact gaactcttgc
    13441 agatgctgcg cccagtacat ctgaatgccg ccttcaacag ctgccggacc aaagataaac
    13501 ggtccgtaga tgaagtggtg taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa
    13561 acccacaccc caagggcacc cgccgaacgc aggaacgctt gcagagattc aatccccatt
    13621 tgtacttttg gccagccgag caacgtcagc caggcgcagg ggatcatcac caggaaggcg
    13681 ataatcacca cataagatgt cccctggaaa atgccgagaa acaccggcag ttttttatcg
    13741 aacagacggt tatgcaacgc cgtcacaatg ccggaaatga taattgcgcc gataatgctg
    13801 gtatcgaggg ttttaattcc cgccatcatt gtcagcccgc tacctgccac cgcgtcctga
    13861 gtgaaatcga cgccgaagta gcttccccag gtcattccca tcgcgttgat gaaatagttc
    13921 caggtcagga aactcaccat caccgccaga caagcacgcc cctgcgcttg cttcgcaagg
    13981 ccaatgggta aaccgacagc aaaaatcagc ggcatattac ggaataccgt ccaaccgccc
    14041 tcttcaataa tatgtacgat ttgcgcgaat aaactgttcg gatcggtcag tgattccccg
    14101 acaaacatcg ggttttgcag caagatggca agacccacca caatcccggc gaagggaaac
    14161 agcagcactg gcgtgaacat cgcgccgcca aagcgttgaa tttgactgag catttccaca
    14221 tcctcttttc tcaattctgt aagtaggaag cctgattggt tatgcggcag gcctggagcg
    14281 aggataggaa gatgcgatcg gcagaacatg acgacccagt gggattcgtg atcgctttca
    14341 tgcttttcgt tgggcggtag agatctacgt tttcgcaaaa aagtagacat ctgtgagcac
    14401 aggtacacag catcgaacac atctttaaaa aaaagatttt ttttcaatcg attaagcaga
    14461 acttgtgggc gcattacccg ggcttgcagg caaaaaagag atctagagat gatctacaaa
    14521 agcattgcgg agcgattaag aattcgactt aattccgcag atttcacgct aaacagcctt
    14581 cttcccggtg aaaaaaagct ggcggaagaa tttgcggtat cgcggatgac catccgtaaa
    14641 gccattgacc ttctggtggc gtgggggctg gtggtccgcc gccacggcag cggtacttac
    14701 ctggtgcgta aagatgtatt gcatcaaacc gccagcctga ccgggctggt ggaggtgtta
    14761 aaacgccagg gaaaaacggt caccagccag gtgctgattt ttgaaatcat gcctgcgcct
    14821 ccggccattg ccagccagtt acggattcaa atcaacgagc agatctactt ctcccgtcgc
    14881 gttcgttttg tggaagggaa accgctgatg ctggaagaca gctatatgcc ggtaaaactg
    14941 ttccgcaacc tatcgctgca acatctggaa gggtcgaagt ttgaatatat tgaacaagag
    15001 tgcgggattc tgattggcgg taattatgaa agcctgacgc cggtgctcgc cgatagactg
    15061 ctggcgcggc aaatgaaggt agcggaacac acgccactgc tgcggatcac ctcgttgtca
    15121 tatagcgaga gcggggagtt tttgaattat tcagtgatgt tcagaaatgc cagcgaatac
    15181 caggtggagt accatttacg gcgactccac ccggagaaga gttaaccgat actccagaag
    15241 agcaccgcca gtaattgggg ggtgataatg cgcaggaaca tcaccaacgg atagacagtg
    15301 gcgtaagaga gcgccgccgc accgctcgtt ggatgaagat tattagcaaa cgccagcgcc
    15361 ggtggatcgg tcatggagcc agccagcatc ccgcacatgg tcaggtaatt cattttggct
    15421 aacatccgcg ccagaatacc aacagtaatc agcggaacgg cggtgatcag cgcaccataa
    15481 ccaatccagc ttagcccttc gccattgacc agggtattca caaaatcccc accagatttc
    15541 agccccacta ccgagaggaa cagtacgatc cccagctccc gcagcgcgag gttggcgctt
    15601 ggcggcataa accagtacag cttaccgata ctgccgatac gcccgaggat caacgccata
    15661 atcagcggac cgcccgccag ccccagtttc aacgcggccg ggaatcccgg cacaaagacg
    15721 ggaatagagc ctaacaatac gccaagcccg ataccaataa acaccggcaa catctgaacc
    15781 tgttgcagtt tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga
    15841 cgccccacca ggttgagaat atcgccgaac tgcaggctga tatcgccact ggcaaccagt
    15901 tcgaccccgg cacggttcag gcgcgagata acaacgtcat agcgttcttt aaagtgcagg
    15961 tcgcgaatac gcttaccaag cacgttttca ttggtgacca ccacacgctc gacgcgtaaa
    16021 tcagtgcctt tcgtggacag cgaggtatcg acctcctgac caatcaccag ttgcgcatta
    16081 tgtaaatccg ctggctgccc caccagatgc agcaaatcgc ccagttggat aatggtatct
    16141 ggcgaaggta cttttagggt ttcttcgcgt ttcagacgcg agcagataat tttgtcgccg
    16201 ttgagaatcg gtacatcttt aatggctaaa tcatgcaggt tagggttctc aacgcgaata
    16261 ttgatagtcc tgatcagcgc gccgccattg gtgcgtgaag actcgtgctg ctgggcttcg
    16321 gtctcgacat tgacgcggaa aataacccgc aacatccaca tggtgaacaa aatcccgcaa
    16381 atgccgaatg gatacgccat cgcgtaactc atccccatct gatcgaccat ttccattggt
    16441 gtacccaggt cgcgcaagat ctgctgccct gcccccagcg ctggcgtatt ggttaccgca
    16501 ccggagaaaa tccccagcac taccggcagt ggaatatcaa acagtttatg caggatggcg
    16561 gtaaccagac caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag
    16621 acgcgcaatg aggcaaagaa gcccggaccc acctgaatcc cgatggtata aacgaacagg
    16681 atcaggccaa attcctgaat aacatgcagc atatcgctac ttaatgtcat ccccgcctga
    16741 gaaacaaaat ggccgacgat gatcccacca aacagcacgc cgccaatacc taatcctatg
    16801 ccgcgaaatt tgacgttgcc gataaacaaa ccgacgactg ccaccaaagc cagaatactg
    16861 accgttaatg ctatatcact catcgtccat ttctcttgag ataacatttt aattgctacg
    16921 gattatggct gaatcggtga agcagatttg ccttatagcg cacattatca cgggaaagaa
    16981 cgtgccgaaa tatcttaaac aggcgaaaat taacgcattg ttgaagaaga aagacaaaaa
    17041 aagccccacc agaatggcgg ggcaaagaga atagctagtt agctatttaa cgcgggacgt
    17101 tcgctgatag cgatacgctg cgctgcgatg ggttcaggct cattacgaat taaatcaata
    17161 tgcagtaaac cgtttacgaa ggttgcacca gagacttcca tattttcagc cagcgtaaag
    17221 ctcaggctaa atggctgatt cataagccct tgatgcagcc attttttctc ttcttttggc
    17281 tgctccggcg tgccttttac gctcaggcgc gttccttcca gttgaatctc taaatcttcc
    17341 tgacgaaaac ctgccagcgc aagggtaatg cggtagtggt tatcgtcgct tttctcaatg
    17401 ttgtacggcg ggaagctctg gctttcaccg gcgttttgca gtgcgttggc cagtttgtca
    17461 aaaccgatcc attgacgcat cagtggggat aaatcgaagt tacgcatagt catttctcct
    17521 tctaagaagc gagtaagtac ctgcaaatcc gaagattcgc atatgctccc tgacggcgag
    17581 catggagatg tcaggccgcg ccaggcggcc ttaaggaatt agttgatttc gatacggcgc
    17641 ggttttttcg cttccggaat cacgcgttcg agatcgatat acagcagacc attcaccagg
    17701 ttagcaccac gaacatgaat gttctcagct aactggaatt tgcgttcaaa gttgcgttca
    17761 gcgatgccct ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc acctttcacc
    17821 accagcagat tatcctgggc ggtaatttcc agttcgctct cagcaaaacc agccacagcg
    17881 atagcaatgc ggtaatggtt ttcgtctacc agttcaacgt tatacggagg gtagccgcca
    17941 ttactctggc tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc aatagcagaa
    18001 cggtaaagcg gggataaatc aaagttacgc ataatcaata gctcctgaaa tcagcgagaa
    18061 tgtaagacct tccacaatgg acaggtcagg tagccagaac acccatcagg cgcgtcctca
    18121 tcggctacga tgtaaaaatg ggtctggaaa tgaacttttc aagccttatc agcggacttt
    18181 tttttgcagt ttatggtcta ttgcatagac tgagggggca gcacgaaacg ttaaagtgcg
    18241 atagccgcca cagtgcgacg ttatgatgat gttattttca gcaagggatc gctataactc
    18301 atcaggccaa cacccacgat caacagatga atttattatg ataagaaatg tgttgttagc
    18361 gttcatgata tgcagcggaa tgacattact cggaggatgc tccagcgtga tgtcccatac
    18421 cggggggaaa gaaggaacgt atcccggcac ccgcgccagc gccaccatga ttggcgacga
    18481 tgaaaccaac tggggcacta agtcgctggc gattctcgat atgccgttta ccgctgtgat
    18541 ggacacgctt ttactgccgt gggatgtgtt ccgcaaagac agctcggtgc gctcgcgcgt
    18601 tgaaaaaagc gaagccaacg cgcaagccac caatgcggtt attccacccg ccagaatgcc
    18661 tgataactga tttagcgctc agtttcggtg atccacagct ggccaccttc catccacgcc
    18721 agccactgac catccggcga gaagaccacg gcgtccgcag aaggtggatt ggcgtggttt
    18781 tcggttaaat actcaacctc gccactttgc gcatgggcac aagcaattcg attatccagc
    18841 acaaagccca accattctcc tgacggatgc cagttaaatg cagactgaat atccgttttg
    18901 ttatgggtta actggcgcgg ctcgccgccc tgtggcgaga taagccacag ttgcacaatg
    18961 ccgttatcat cacgcattaa aaacgcgatt tgcgtaccct gcggattaca gcgcacccag
    19021 tggcgcggga cgttgactaa ccccggataa gcccgatggt gggtaaaggt taaacgtcgc
    19081 tgcacaacgc cacgcggtgg cgcgggcagg gttgtttccg ttccgcttaa cggcgcatca
    19141 cctgctactt tccagccagc ttcatcttgc ggtaactcaa cgataaacag ctccggcact
    19201 ttctcgccct ttggcgaaag tgtatcgcca ataaacgcca gcgcgtgatt tcctacccat
    19261 ccttcttcat aagcacgatt gatttcatta ctgccaggct gcggcgtggg cgtggtttta
    19321 cttaccagca cgcaccagtg gctgccgctg tattcacgcg gatgctgctt ttgtatgttg
    19381 accgggccaa acggtgcagc aataccgacg tttcgcaaat ccagcgccgg atcgagttca
    19441 tgcattacat ggtcgttata ggtaaagctc accctttcac cgttcgggct aaagacatgt
    19501 acatgactgc cgccgcgcag cgctcctgga gtgtacggtg cggtaatatc cattgcatcg
    19561 agattgctca ccttgccgct ttcagcaatc acgccgcgac gatgatggaa atcgtaatgc
    19621 catgtttcat caggattttc aggtccgtgg atgaagacat atttttctga cttcggatga
    19681 acggtcacca cgccgacgcg tgcgccctgt gacgcgcgat agataacctc gacctcgccg
    19741 gtatggatat tcacacgctc aatggtctcg ccggtaaacg acgcgccaga aggacgcacg
    19801 tcaaatacca gccactggct gtcgggcgtc cagatattgg tattggtgag caggtgatta
    19861 cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggg tcccgccatc
    19921 atacttcaca aggacttcac tttcagacaa tagtctttcg ccatcgtgat gaatgtgagt
    19981 gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg gtcggcattg
    20041 gcgcgaaagt tagccggcaa aatgcaggtg atcgcgctgg ataaaaagca ccagcatggt
    20101 actgaaggtt tcagcaaacc ttgtggcggt ctgctggcac cggacgcgca gcgatctttt
    20161 attcgcgatg gactgacgct tcctgtcgat gtgatcgcca acccgcagat tttcagcgtc
    20221 aaaaccgtcg acgtcgccgc attgctcacg cgtaactacc agcgaagcta tatcaatatt
    20281 aatcgccatg ctttcgactt gtggatgaaa tctctgatcc ccgccagcgt tgaggtttat
    20341 cacgacagcc tgtgtcgaaa aatctggcgt gaggatgata aatggcatgt catttttcgt
    20401 gcagacggtt gggagcagca tatttctgcc cgctatctgg tcggtgccga tggtgccaac
    20461 tcgatggtgc ggcgacatct ctacccgaac catcaaattc gtaaatatgt cgctatccag
    20521 cagtggttcg cagagaagca tccggtgccg ttctactcct gcatctttga taatgcgata
    20581 actgactgtt actcatggag tatcagcaaa gacggttatt ttatctttgg cggtgcctat
    20641 cccatgaaag acggtcagac gcgtttcaca gcactgaaag agaaaatgag cgcctttcag
    20701 ttccagtttg gcgaagcggt gaaaagtgag aaatgtaccg tgttgtttcc ctcacgctgg
    20761 caggattttg tctgcggtaa ggacaatgcc tttctgattg gtgaagcggc gggatttatc
    20821 agcgccagct cgctggaggg gattagctat gcgctggata gcgcagagat tctgcgttcg
    20881 gtgttactga agcagccgga gaagagtaac gccgcctact ggcgcgccac ccgcaaactg
    20941 cgtttaaaac tcttcggtaa gatagtaaaa agccgatgcc tgaccgcacc ggctttaaga
    21001 aagtggatta tgcgcagtgg tgtggcgcat attccacagt tgaaagatta tccaacgcgt
    21061 ttcacatcgc ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac ggcggagata
    21121 taaaccagtg ccggggcgaa accgtaaccc tgcgccaggt accccaccac cagcggaacg
    21181 gtgatgccgc ccagaccacc ggcaaagtta aacacgccgc cggttaaacc aatcaggcgc
    21241 atcggtgcca gagaagagac cagcgaccag gtaatcgaag caaaaccgtt gccgaagaat
    21301 gccagcgcca tcaggcacat aatcatcatc gggtcattgg tgtagttagc gcccataatg
    21361 caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc cagtgaaaag
    21421 cccttacgta ccagcagatc cgcgacccag ccagagagca ggacgccgac aaacgccgcg
    21481 aggaatggca cagtggtcat aaagcctgct ttcagcgccg tgattccttt ttcctgggtt
    21541 aaatagttcg ggaaccaggt taagaaaaac cacagtgtag aagccaccgc aaattgccca
    21601 agataaacgc cgatcagttt acgatggaac accagcttcc agtctttggc tgttaacggc
    21661 tgacgtgcct cttttttcac cggcgcatca ccatccacca gaccgccgcc atcacgaatg
    21721 taatccagtt cagctttgct gataccttta gtcaggcgcg gcggctgata aaccttaaac
    21781 caaatcagcg accagataat gccgatacca ccggtgacaa tgaacaccca gtgccagctc
    21841 aacatttcct gaatccagat cagcagcgga gtcagaaacg ccagaccgac aaactgacca
    21901 gacgtataaa aaccaacggc ggaagcgcgt tcatgttccg ggaaccagct ggtcaccatc
    21961 cggttattgg tcgggaaagc gggcgcttcg aaaataccgg ttatcgcgcg cagaccaatt
    22021 aatgacatta atcccgtggc aaagccctgg aataaagtcg ccactgacca gccaaatatc
    22081 gcaataaaat aagtcacgcg tgaacctacg cgatctaaga accaaccgcc ggggatctgg
    22141 cataacgtat aaagccatgc gaaggccgaa aatacatagc ccatttccgc tttcgtaatg
    22201 ccgaactctt cctgaatatg agcggaagcc acggccaggt tagcgcggtc aacataacaa
    22261 atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc cggctttgct
    22321 gcattaacgg gaatatccat agcgagcttt ctccagattt tgggcatagc gaagccgctc
    22381 accatgccct gtaatttaca gagggttata tttttgtatt actgttttag tgcccgatga
    22441 ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga cgccagagcg
    22501 gattacgcca gtccggggca tttttactga actcaataac tttagcttcg tcgatttcca
    22561 cgcctaagcc cggtttcgtt agcggtttaa agaagccgcc aaccatgctg aagtcttctt
    22621 tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc atactttgtt
    22681 cctgaagtac ggcgttatag gaaacaaagt cgatatgcag acaagccgcc agtgcaatcg
    22741 gtccgagcgg acagtgcggc gcaagtgtca cgtcataggc ttctgccatt ccggcaattt
    22801 tgtagcattc ggtaataccg cctgcatgag agagatccgg ttgcagaatc gaaataccac
    22861 ccgcctccag cacacgttta aaatcgaaac gtgagaacat gcgctcaccc gccgccagtg
    22921 gaatatgcgt ttgtgccgcc agtttcgggt agtattcggc ttgttccgcc agcaccggct
    22981 cttcaataaa cagcgggcga tacggctcca gttctttaat cagcactttc gccatcggcg
    23041 cgctgacgcg accgtggaaa tcaagaccaa actcaatctg attgccaaaa gcttcacgaa
    23101 tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt agccccagct
    23161 cttcacaacc gttcagtttg aaggtatcga agccgatttc gcgcagggtt tttatgccgt
    23221 cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt ttgtcgcgaa
    23281 ccaggccgcc catcagttgc cacaccggcg cattcagcac tttgcctttg atatcccata
    23341 acgcctggtc gatcccggcg atggcgctca tcaggattgg accgccacga taaaatccgg
    23401 cgcgatacat cacttgccat aagtcattga tgcgcgatgg atcctgacca atcaaatagt
    23461 cacccagctc gtgaactgcc gcttccaccg tacgggcgcg gccttcaatc acgggctcgc
    23521 cccagccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg ggaggtaaac
    23581 gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct taacaaatgc
    23641 tgctgcctgc tgcgcggtgc gttctatgga ctgcccggcg cgatagagat cgctgcctaa
    23701 gcctgcccct gcacaacctg cgtctatcca ctgcgccagg ttttctggcg tcacgccgcc
    23761 aacggcaaag actgcgatgt ccggtggtaa taccgctttt aacgctttga tgtattgcgg
    23821 accaaaagcc gatgacggaa atattttcag cgcctgagcg cccgcgtcga gcgcggtaaa
    23881 ggcttcggtc gccgtcgcgc agccggggca aacggtcatg ccgtagccca ccgcacggcg
    23941 gatcacttca ctatggatat tgggcgtaac gatgagctgg cagcccatcc tggcgagcac
    24001 atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt cgccatacgc
    24061 atcaacgatg gcgggaatgc tctgctccca ttgtggggaa ttcagcggga tttcaaccgc
    24121 gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg gcgtaatgcc
    24181 gcgcaaaatg gcgatcagcg ggagtttagt ttgccactgc atgagcgatg ctccttatac
    24241 cagcctgaaa tgccgtgtcg cccgccaccg tcgccacgtc gcaacccatc gcctgaaagg
    24301 cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg tgttgatggg
    24361 tcacatagtc gcgcatactg gcaacctctg cgccaatcaa caaaccagag agaaattcac
    24421 tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca aaaagctgcg
    24481 gcaatatggc gggagcatta aggccacgct caaggccagc cgcgaaggca tcggcagagt
    24541 tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa tgatgtaatt
    24601 caccggtcat cacggtgcga aaatcgttga tttgctggct atcggcctgc acccatttgc
    24661 aatgggttcc gggcataaca taaagagagg aaggagccaa agtgcgcgcg ccgatcaatt
    24721 gtgtttcttc gccgcgcatc acattatggt tatcgtcatg agagacgcat aatccgggaa
    24781 taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa aaacgggcag
    24841 gaacggataa atacggtgca actttccagc cgacattgct gccgaccatt cctgccatca
    24901 ccactggcgt attctcttca tgccagtcgg tcgtcacttc tgctaacacc gcagccggag
    24961 attttccgtt caggcgcgtg acgcctgctt ctgattgcct gctgtccagg cagtggtcgc
    25021 cctgataaag ccaggcgcgc agattggtcg atccccagtc aattgcgatg tagcgagctg
    25081 tcatgtgatt tcctttaacc ttcgtgtcga gctggcgatc atggtaagcg ccgcctgctc
    25141 tgccgcatcg ccgtcctgat gccgtattgc atcgaacagc gccttatgtt cctggagcgt
    25201 ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc gctgcagcga
    25261 actgatcgca atgctaagtt gctgtaacac cgggttatgc accgactgca gcaccgcctc
    25321 gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg caatcatctc
    25381 gttcagcgcc gattcaatct gcgccagatc gctggaagtc gcgcgttctg ccgcccaacg
    25441 ggcaatcgcc ggttccacca gatttcgcac ttcgctcatg gcactgataa gccgtgggtc
    25501 gtagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc actggttacg
    25561 cggtgccaca aacgcgccgc gataacgttt catttcaatc agccgcttcg ccatcagcga
    25621 gcggaacacc tcacggatga tgttgcgcga ggttgcaaac tcctcacaga gttccgcctc
    25681 agccggaagc ggcgagcctg gcacgtattt gccgtgaacg atctgtttgc ccagcgtaat
    25741 gacgatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt atgttcttct
    25801 ctactttacc ccgatcgatg cataacgcgg caactttgta gtaccagcgt gatgacgttc
    25861 gcgtttgctg tgtgtgtaat gtagtacaaa cttatattgt tgtactacaa tttagatcac
    25921 aaaaagaaca atgcataaaa aaatgacatg cgtcgggcag aaatctgaaa agggatgtca
    25981 ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagctact cgtttaaagg
    26041 attaatcatg aagttgaatt ttaagggatt ttttaaggct gccggtttgt gttctttggc
    26101 atttgcgttg accggatgta tcacctgggg attggtttcc aatactgcaa gccgtccaca
    26161 tgccgagcaa tggcggtcag acacgattaa gggtttatcg ctggctgaag acagtaacgg
    26221 tacgaaaggc tatgtttttg ttggtgaatc gttggactat ctgttgacca ccggcggcgg
    26281 tgaggtggtt aaaatgctga acgatccggc aattcatggt gaaagaatca ccgtttctga
    26341 taacgcgaaa tttattttaa gcagcagtaa taagaaattc tctggtgcta ttacgctcta
    26401 ttacgactgg aacaacgaag aagataaggc actggcaaca caatatggtt ttatttgtga
    26461 tacccggcgt tgtacatgga tgctggacgg tttgaaaggc tcaatccacc agaaaaataa
    26521 aaaagcggat tactcaaacg tgatggtttt ccatcaaccc tttacggtgg gattttatga
    26581 atacaaagca acagatggcg tcccgcgcgg cctcgttaat gcgctgctgc cagtcactct
    26641 tacgctggat atagtaacgt caccactcca gttcctgatt ttatgcacga ccagaaattg
    26701 ttaatcgctg cgggcaaacg cattcatgcc gtttgcccgt tcactgatta attcagcaca
    26761 tacttctcaa tagcaaatgc cacgccatct tcaaggttgg atttggtgac aaagttcgcc
    26821 acttctttca ccgaaggaat agcgttatcc atcgccacac cgacgcctgc atattcaatc
    26881 attgcgatgt cgttttcctg atcgccaatc gccatgattt cttccggttt aatacctaac
    26941 acgtcggcca gtgatttcac acccgtacct ttgttaacgc gtttatcgag gatttcgagg
    27001 aagtacggcg cacttttcag cacggtgtat ttttctttca cttcctgcgg aatacgcgca
    27061 atggcctggt cgaggatggc gggttcatca atcatcatca ctttcaggaa ctgggtattg
    27121 gggtccattt tctccgcttc gcagaacacc agtggaatgg tggcaacgaa ggattcatgc
    27181 accgtgtagt agctgatatc tcggttggcg gtgtacagcg tggtgcggtc cagggcgtgg
    27241 aaatgagaac cgacttcgcg ggagagtttt tccaggaaac ggtagtcgtc atagctgaga
    27301 gcggtttgcg ccacagtgct accatcggcg gccttctgta ccagcgcgcc gttataagta
    27361 atgcagtagt cgcccggctg ttccatatgc agctctttca ggtagttgtg aacgcctgca
    27421 taagggcgac ccgtcgttag cacgacattc acgccacggg cgcgagctgc ggcaatcgca
    27481 tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa gggtgccatc catatcgata
    27541 gcaatgagtt tgatagccat gagttcccca ggtagattgg ttcctgaccc atgctaacgc
    27601 gattccgctc aaaaatcagt acaacacccg aaggaaaagg gggatgcaac gcgcgttgcg
    27661 tgctcccctt ttgcttagcg gaagagtttc cctttcagca gttccatacc tgcggaaagc
    27721 agatcgttat tggcttgtgg tgacacttca ccttgcggtg agagtgcatc aataatcttc
    27781 ggcaattgtt ctgccagtaa actggaagct gtactggtat ccacgccaag tttttgtcca
    27841 agatcggata ccgcgtttga gccgagtgct gattccagtt gctcgccgct aactggttgg
    27901 ttgccctgtt gattactcaa ccaggttgag agaatggcac ctaagccgcc actttgcagt
    27961 ttttccagca gcacctgaat gccgccctgc tcctcaaccc aacttaaaat agcctgatat
    28021 ttccccgcat cgcctttcag aaaggcaccg acaacttcat caaaaagccc catgataatc
    28081 acctgtaaag cgttacgtgt tgactcaaaa agtatagatc tgcgggatga taattgcgga
    28141 ttgcagaaat aaaaagggcg aagatgatct ccgccctttt cttatagctt cttgccggat
    28201 gcggcgttaa cgtctgatcc ggcctacaaa atcatgcaaa ttcaatatat tgcaggagtc
    28261 tcgtaggcct gataagcgta gcgcatcagg cacgctcgca ttgttaacgt cattaaatat
    28321 cgatattcgc cgctttcaga gcgttctctt caataaacgc acggcgcggt tcaacggcgt
    28381 cgcccatcag cgtggtgaac aactggtcgg cagcaatcgc atctttaacg gtaacgcgca
    28441 acatacggcg gctttccggg tccatagtgg tttcccacag ctgttccggg ttcatctcgc
    28501 ccagaccttt ataacgctgg atggagaggc cgcgacggga ctctttcacc agccagtcca
    28561 gcgcctgctc gaagctggct accggctgac gacgctcgcc acgttcgata aacgcatcgt
    28621 cttccagcaa gccgcgcagt ttctcgccca gcgtgcagat acgacgatat tcgccaccgg
    28681 tgataaactc gtgatccagc ggatagtcag tatccacacc gtgggtacgc acgcgaacaa
    28741 tcggttcgaa caggttttgt tcggcattgg tgtggacatc aaacttccac tggctgccgt
    28801 gctgttcttt gtcgttcagt tcgctgacca gtgcgttcac ccagcgggta acggtctgtt
    28861 catcagagag gtcagcttcc gtcagcgtcg gctgatagat aagctctttc agcatagctt
    28921 tcggataacg acgctccatg cggttgatca ttttctgcgt cgcgttgtac tcagacacca
    28981 gtttctctaa cgcttcgcca gccaatgccg gtgcgctggc gttggtgtgc agcgttgcgc
    29041 cgtccagcgc gatagagatc tggtactgat ccatcgcttc gtcgtcttta atgtactgtt
    29101 cctgcttgcc tttcttcact ttgtacagtg gcggctgagc gatatagacg tgaccgcgtt
    29161 caacgatttc cggcatctga cgatagaaga aggtcaacag cagcgtacga atgtgcgagc
    29221 cgtcaacgtc cgcatcggtc atgatgatga tgctgtgata acgcagtttg tccgggttgt
    29281 actcgtcacg accgataccg cagccaagcg cggtgataag cgtcgccact tcctgggaag
    29341 agagcatctt atcgaagcgc gctttctcga cgttgaggat tttacccttc agcggcagaa
    29401 tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc gcccgcggag tccccttcca
    29461 caaggtacag ttcggaaagc gccggatcgc gttcctggca gtctgccaat ttgcccggca
    29521 ggcccgctaa gtcgagcgca cctttacggc gggtcatttc acgcgcgcga cgcgccgctt
    29581 cacgggcacg ggcagcatcg ataattttgc caaccacgat tttcgcgtca gttgggtttt
    29641 ccagcaggta ttccgccagc agttcgttca tctgctgttc aaccgccgat ttcacctcag
    29701 aagaaaccag tttgtctttg gtctgggagg agaatttcgg gtccggcact ttcacggaaa
    29761 cgaccgcaat caggccttca cgcgcatcgt caccggtggc gctgactttg gcttttttgc
    29821 tgtagccttc tttgtccatg taggcgttca gggtacgggt cattgccgca cggaagcctg
    29881 ccaggtgagt accgccgtca cgctgtggaa tgttgttggt aaagcagtag atgttttcct
    29941 ggaagccatc gttccactgc aacgccactt cgacgccgat accatctttt tcggtggaga
    30001 agtagaagat attcgggtgg atcggcgttt tgttcttgtt cagatattca acgaacgcct
    30061 tgatgccgcc ttcatagtgg aagtggtctt ctttaccgtc gcgcttgtcg cgcagacgaa
    30121 tggaaacgcc ggagttgagg aacgacaact cacgcagacg tttcgccaga atttcatatt
    30181 cgaactcggt cacattggtg aaggtttcga ggcttggcca gaaacgcacc atggtgccgg
    30241 ttttttcagt ctcgccggta accgccagcg gagcctgcgg tacaccgtgt tcgtagatct
    30301 gacggtgaat tttaccctcg cgctggataa ccagctccag tttttgcgac agggcgttta
    30361 ctaccgaaac accaacgccg tgcagaccgc cggacacttt ataggagtta tcgtcaaatt
    30421 taccgcctgc gtgcagaacg gtcatgatca cttccgccgc cgatacgccc tcttccgggt
    30481 gaataccggt cggaatgccg cgcccgtcat cctgtacaga gacagagttg tcggcgtgaa
    30541 tggtgacgat aatttcttta cagtgacccg cgagcgcttc gtcgatagcg ttatctacca
    30601 cctcgaatac catgtggtgc agaccggtgc cgtcatccgt gtcgccgata tacatacccg
    30661 ggcgcttacg caccgcatcc agccctttca ggactttgat actggaggag tcataagaat
    30721 tcgacatcaa cgtttctcgc tcatttatac ttgggttaat ccgttatttt acccttttcc
    30781 acggtaaaca tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc actgatcgcg
    30841 ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg caagcaaccc gcgacgctca
    30901 tcatcaagct cagaggcaaa atcatctatc aggtagagac accgccgccc gctttcacgg
    30961 gtgaggaact ctccttgcgc cagacgtaag gcgcacatca acagcttaag ctgcccacgc
    31021 gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg
    31081 ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat tacgttccag cacctcagca
    31141 tattctgtct ctttctccca gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga
    31201 aattgcttac aggtatcagc catatcggcc gcgataccgg cgctatactc cgcgcgccag
    31261 gtactgattt gctccgccag cgggatcagt tctttatccc acgggcgtag ctgttcgtaa
    31321 cgtgtcacct ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag attgctccag
    31381 gcggtgaaaa atccgggttc gttgtgaaag catccccagt cgaggaatgc tcttctgtat
    31441 ttggggccgc cgttgagtaa agtaaaccct tcaggcgtta tcagttgcat cggcatcagg
    31501 tgcgccagct ccgcgacttt atgcccgtca gtaccgtcga tgcggacttt gctgtcgccc
    31561 tgtttgtctt tggttaagcc aatcgccgtc tcgcgctctt cgccctgtaa tcgcccgtgg
    31621 agaacaaacg cctcctgctc gtggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc
    31681 cgaccatggc cgagcgtata gatggcttcc agcacgctgg ttttgccact accgttggca
    31741 cctaccagaa agttaaagcc gggagataaa gcgagatccg cggtttcgat attgcggaaa
    31801 tcgcggatca acaagcgggt gagggacatt acagtctcat tggcatgaca acataggcag
    31861 ccgactgtga tgcggcatct tcaatctgca cgctggaaac cgaatcggtc agcatcatgc
    31921 gcacgttttc acatttcagc gcgttcagaa catccagcac atagctgacg ttgaagccga
    31981 tttccatctc cgcaccgcta taggtaacgt cgaggatctc ttccgcttct tcctgttccg
    32041 ggttgttggc ggtgattttc agctggtttt cgctgacata cagacgtacg ccgcggaatt
    32101 tctcgttaga gagaattgcc gcgcgagcaa acgcctgctt gaggagatcg caacctgctt
    32161 ccagatgttt gtccgggttt ttcggcagaa cgcgacggta atccgggaag cgaccatcca
    32221 ccagtttcga ggtgaagata aagtcgccaa cgtgggcgcg aatgttgttg ctgccaatct
    32281 gcacgcgcag cggattgtcg ccgccgtcga gcatacgcat cagttcaatc acgcctttac
    32341 gcggcacgat caccgaatgg cttggcaaag attgaccaat tggcattgaa cagactgcca
    32401 gacggtggcc gtcggtcgcc acggtgcgca gttcttcacc ttcggtttca aacagcatac
    32461 cgtttaagta atagcgaacg tcctgatgcg ccatagaaaa ctgggtcgct tcaatcagac
    32521 gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct ctgccagtca tcgagattcg
    32581 ggaaatccgc cgctggcagg gtagacagcg aaaaacggct acgcccggag cgcaccagca
    32641 tccgctcacc ttccagctgc acggcaattt ccgcgccttc aggcagacca cggcagatat
    32701 caaagaattt gcgcgctgga acggtcgtcg cccctggttc gtgcggctga accagcgcaa
    32761 cgcgtgccac catttccatc tcgagatcag taccggtcag cgacaacgta ccgtcagcaa
    32821 cctgtaacag cagattaccg agaatcggta gcgtaggacg accacctaac ggaccgctca
    32881 cctgttgtag cggttttaat aaatgctcac gttctacggt aaatttcata ggtttacgat
    32941 gacaatgttc tgattaaatt ggaaaaatct tctttgatat cgtggctctc ttcacgcagc
    33001 tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt cacgaccacc aaacgcatcg
    33061 ccaatctccg gcagactgtg gttagtcagc tctttcgcca gcgccatcgc catctggcgc
    33121 ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt gatcttgtag
    33181 tactccgcca ccgtcttctg aatattgtcg atggtgacca gtttttcctg caatgccagc
    33241 aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt aaagttggcg
    33301 ttagcaataa cgcggttcag cgcgccttcc agctcacgta cgttagatcg tagacgcttg
    33361 gcgataaaga acgccacttc gcccggcaaa cgaatgtcgt tttcgtcggc ctttttcatc
    33421 aggatcgcca cgcgggtttc cagctctggc ggttcgatcg ccacagtcag cccccagccg
    33481 aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg atccgaggtg
    33541 agaatgatct gttgattacc ttccagcagg gcgttgaagg tgtggaaaaa ctcttcctga
    33601 gatcgttctt tattagcaaa aaactgaata tcgtcgatca gcagtgcatc tacggaacgg
    33661 tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca
    33721 aagcgctcgg agtgcatata aaccactttg gcattcggct tgcgcgccat aatgccgtta
    33781 cccaccgcat gcagcaggtg agttttaccc agacccgtgc cgccataaag gaacaacggg
    33841 ttataggcac cgccagggtt atctgccacc tggcgagccg ccgcgcgcgc cagttggtta
    33901 gatttacctt caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga acgataggtc
    33961 ggttctgccg gggccgggac gttatcccaa cctgagcgcg tagaaggcgc agcacgttgc
    34021 ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc
    34081 gtttgcgtca ccggtttggt gccgacttca aaacgcagct gtggggcatc cgctccgcag
    34141 aaactggtta gcagtccatt gatattatta aggtacttgt cccgtaccca atcgaggaca
    34201 aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca gttccgcctg caatgggcgt
    34261 atccacatac tgaattctgt ggctggtaac tcatcctgca atcgggcaag acactgctgc
    34321 caaagcgaaa gtgacacggc ggactccact cgaacaaaag tcgataatga ctaaggctga
    34381 aacattcatg attgttgacg tacgtcgaaa agaccctgca tgagggtgac gcacgaaccg
    34441 ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg cggatcatag
    34501 cctaaactgc gcaagagatc ttctgtttct cacagatttt cccgatttat ccacaggact
    34561 ttccagaact cgctaagtgt aaacgatcct gccgcagggc gggcacgatt tacgccgcat
    34621 attggaaaat ttaatgacca tagacaaaaa ttggcttaat cgatctaata aagatccagg
    34681 acgatccttg cgctttaccc gtcagcccgt ataatcctcc acccggcgcg ccatgctggt
    34741 tttcactggt gtgaggccgt acgttttccc tgcgaaaagg tgcggaaaag cgcggtaaat
    34801 aaggaaagag aattgactcc ggagtgtaca attattacaa tccggcctct ttaatcaccc
    34861 atggcttcgg tgtccatcgt ttcatttttc ggcggatatc caataaagcc attgaattta
    34921 ttcaagttta ggtagaaatc gccatgaaac gcacttttca accgtctgta ctgaagcgca
    34981 accgttctca cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac
    35041 gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa gtaataaagc taacccctga
    35101 gtggttaagc tcgcatttcc cagggagtta cgcttgttaa ctcccagtca attcacattc
    35161 gtcttccagc agccacaacg ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat
    35221 tcgctggggc atccccgtat cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat
    35281 gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc gtctgcgcca acatgaactc
    35341 ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga taaccgtgct
    35401 ctctcggaag cgttggaaaa attatggcgc cgccactgtc gcctggctcg cgggtcctga
    35461 tagccctcat tcgggtctat caacgcctga ttagtccgct actcgggccg cattgtcgtt
    35521 tcactccaac ctgttcaagc tacggaattg aggcattgcg caggtttgga gtgataaaag
    35581 gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc tttacaccct ggtggtgacg
    35641 atcccgtccc gcccggacca tttgatacca gagaacacta acgatggatt cgcaacgcaa
    35701 tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct gggagcagga
    35761 taaaaacccg caacctcagg cccaacagac cacgcagaca acgaccaccg cagcgggtag
    35821 cgccgccgac cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga
    35881 cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt gagcaagctc tgctgcctgc
    35941 ttacccgaaa gagctgaact ctacccagcc gttccagctg ctggaaactt caccgcagtt
    36001 tatttatcag gcacagagcg gtctgaccgg tcgtgatggc ccggataacc cggctaacgg
    36061 cccgcgtccg ctgtataacg ttgaaaagga cgcttatgtg ctggctgaag gtcaaaacga
    36121 actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac acgtttacca aaacgtttgt
    36181 cctgaaacgt ggtgattacg ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa
    36241 accgctggag atctccacct ttggtcagtt gaagcaatcc atcactctgc caccgcatct
    36301 cgataccgga agcagcaact tcgcactgca caccttccgc ggcgcggcgt attccacgcc
    36361 tgacgagaag tacgagaaat acaagttcga taccattgcc gataacgaaa acctgaacat
    36421 ctcttcgaaa ggtggttggg tggcaatgct gcaacagtat ttcgcgacgg cgtggatccc
    36481 gcataacgac ggtaccaata acttctatac cgctaatctg ggtaacggca tcgccgctat
    36541 cggctacaag tctcagccgg tactggttca gcctggtcag actggcgcga tgaacagcac
    36601 cctgtgggtt ggcccggaaa tccaggacaa aatggcagct gttgctccgc acctggatct
    36661 gaccgttgat tacggttggt tgtggttcat ctctcagccg ctgttcaaac tgctgaaatg
    36721 gatccatagc tttgtgggta actggggctt ctccattatc atcatcacct ttatcgttcg
    36781 tggcatcatg tacccgctga ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct
    36841 gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat gacaaacagc gtatcagtca
    36901 ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg ctgggcggct gcttcccact
    36961 gctgatccag atgccaatct tcctggcgtt gtactacatg ctgatgggtt ccgttgaact
    37021 gcgtcaggca ccgtttgcac tgtggatcca cgacctgtct gcacaggacc cgtactacat
    37081 cctgccgatc ctgatgggcg taacgatgtt cttcattcag aagatgtcgc cgaccaccgt
    37141 gaccgacccg atgcagcaga agatcatgac ctttatgccg gtcatcttca ccgtgttctt
    37201 cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc agcaacctgg taaccattat
    37261 tcagcagcag ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa
    37321 aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc gccttttttc
    37381 ttttcgtagg gcggataagc accgcgcatc cgccacacaa agcaacagga acatcatgag
    37441 cgataatgac actatcgtag cccaggccac gcctccggga cgtggcggcg ttggcatcct
    37501 gcgcatctcc ggcctcaaag cccgtgaagt tgccgaaacc gtgctgggta aactgcctaa
    37561 gccgcgctac gccgattatc ttccgtttaa agacgccgac ggcagcgtgc tcgatcaggg
    37621 gattgcgcta tggttccctg gcccgaactc gttcaccggc gaagatgtgc ttgaactgca
    37681 gggtcatggc ggtccggtga tcctcgacct gctgttaaaa cgcattctga ccattcccga
    37741 cctgcggatt gcccgccctg gtgagttttc cgaacgcgcg tttcttaacg ataaactcga
    37801 cttagcccag gccgaggcga ttgcggatct tatcgacgcc agttcggaac aggcggcccg
    37861 ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg gtcaaccatc tggtggaagc
    37921 cctcacccac ttgcgcattt acgtcgaagc ggcaattgat ttccctgatg aagagatcga
    37981 tttcctctcc gacggcaaaa ttgaagccca gctcaatgac gtgattgccg atcttgatgc
    38041 agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa gggatgaagg tggtgattgc
    38101 cggacgtcct aacgccggta aatcgagcct gttaaacgcg ctggcgggcc gtgaagcggc
    38161 aatcgtaacc gatatcgccg gaaccacgcg tgacgtgctg cgtgagcata tccacattga
    38221 cggaatgccg ctgcatatca tcgataccgc cgggctacgt gaagccagtg acgaagtgga
    38281 acgtattggt atcgagcgcg cgtggcagga aattgaacag gccgaccgcg tgctgtttat
    38341 ggtcgatggc accacgacag acgccgttga tccggcagag atctggccgg aatttatcgc
    38401 ccgtctgccc gccaaactgc cgatcaccgt ggtacgcaat aaagccgata taaccggcga
    38461 aacgctggga atgagtgaag tgaacggcca tgcgttgatt cgtctctcgg caaggactgg
    38521 cgaaggcgtg gacgtgctgc gtaaccatct caaacagagc atgggctttg acaccaatat
    38581 ggaaggcggc ttcctggcgc gccgtcgcca cttacaggcg ctggaacaag cggcggaaca
    38641 tctacaacag ggcaaagcgc aactgttggg agcatgggcg ggcgaactgc tggcggaaga
    38701 gttgcgcctg gcgcagcaga acttaagcga aatcaccggg gaatttactt cagacgacct
    38761 gcttgggcgg attttctcca gcttctgtat tggtaagtaa ctgcgcttac gaagccgtat
    38821 tctgactgtc agatgcggct tcgcttcatt gttagcactc ctgttatgcc tcaccccttt
    38881 tttaaacatt aaaattctta cgtaatttat aatcttaaaa aaaaacattt aatattgctc
    38941 cccgaacgat tgtgattcga ttcacattta aacaatttca gaatagacaa aaattctgag
    39001 tgtaataatg tagcctcgtg tctttcgagg ataagtgcat tatgaatatc ttacatatat
    39061 gtgtgacctc aaaatggttc aatattgaca ataagattgt cgatcatcgc ccttgatttg
    39121 cccttctgta gccatcacca gagccaaacc gattagattc aatgtgatct atttgtttgc
    39181 tatatcttaa ttttgccttt tgcaaaggcc atctctcgtt tatttacttg ttttagtaaa
    39241 tgatggtact tacatatata tctggcaaat taatcggtat agcagatgta atattcacag
    39301 ggatcactgt aattaaaata aatgaaggat tatgtaatgg aaaactttaa acatctccct
    39361 gaaccgttcc ggattcgtgt tattgagcca gtaaaacgta ccactcgcgc ttatcgtgaa
    39421 gaggcaatta ttaaatccgg tatgaacccg ttcctgctgg atagcgaaga tgtgtttatc
    39481 gatttactga ccgacagcgg caccggggcg gtgacgcaga gcatgcaggc cgcgatgatg
    39541 cgcggcgacg aagcttacag cggcagccgc agctactatg cgttagccga gtcagtaaaa
    39601 aatatctttg gttatcaata cactattccg actcaccagg gccgtggtgc agaacaaatc
    39661 tatattccgg tactgattaa aaaacgcgag caggaaaaag gcctggatcg cagcaaaatg
    39721 gtggcgttct caaactattt ctttgatacc acgcagggcc atagccagat taacggctgt
    39781 accgtgcgta acgtctatat caaagaagcc ttcgatacgg gcgtgcgtta cgactttaaa
    39841 ggcaactttg acctcgaagg attagagcgc ggcattgaag aagttggccc gaataacgtg
    39901 ccgtatatcg ttgcaaccat caccagtaac tccgcaggcg gtcagccggt ttcactggca
    39961 aacttaaaag cgatgtacag catcgcgaag aaatacgata ttccggtggt aatggactcc
    40021 gcacgctttg ctgaaaacgc ctatttcatc aagcagcgtg aagcagaata taaagactgg
    40081 accatcgagc agatcacccg cgaaacctac aaatatgccg atatgctggc gatgtccgcc
    40141 aagaaagatg cgatggtgcc gatgggcggc ttgctgtgca tgaaagacga cagcttcttt
    40201 gatgtgtaca ccgaatgcag aaccctttgt gtggtgcagg aaggcttccc gacatatggt
    40261 ggtctggaag gcggcgcgat ggagcgtctg gcggtaggtc tgtatgacgg catgaatctg
    40321 gactggctgg cttatcgtat cgcgcaggtg cagtatctgg tcgatggtct ggaagagatt
    40381 ggcgttgtct gccagcaggc gggcggtcac gcggcattcg ttgatgccgg taaactgctg
    40441 ccgcatatcc cggcagacca gttcccggca caggcgctgg cctgcgagct gtataaagtc
    40501 gccggtatcc gtgcggtaga aattggctct ttcctgttag gccgcgatcc gaaaaccggt
    40561 aaacaactgc catgcccggc tgaactgctg cgtttaacca ttccgcgtgc aacatatact
    40621 caaacacata tggacttcat tattgaagcc tttaaacatg tgaaagagaa cgcggcgaat
    40681 attaaagggt taacctttac ctacgaaccg aaagtattgc gtcacttcac cgcaaaactg
    40741 aaagaagttt aattaatact acagagtggc tataaggatg ttagccactc tcttacccta
    40801 catcctcaat aacaaaaata gccttcctct aaaggtggca tcatgactga tcaagctgaa
    40861 aaaaaacact ctgcattttg gggtgttatg gttatagcag gtacagtaat tggtggaggt
    40921 atgtttgctt tacctgttga tcttgccggt gcctggtttt tctggggtgc ctttatcctt
    40981 atcattgcct ggttttcaat gcttcattcc gggttgttgt tattagaagc aaatttaaat
    41041 tatcccgtcg gctccagttt taacaccatc accaaagatt taatcggtaa cacctggaac
    41101 attatcagcg gtattaccgt tgccttcgtt ctctatatcc tcacttatgc ctatatctct
    41161 gctaatggtg cgatcattag tgaaacgata tcaatgaatt tgggttatca cgctaatcca
    41221 cgtattgtcg ggatctgcac agccattttc gttgccagcg tattgtggat aagctcgtta
    41281 gccgccagtc gtattacctc gttgttcctc gggttgaaga ttatctcctt tgtgatcgtg
    41341 tttggttctt tcttcttcca ggtcgattat tccattctgc gcgatgccac cagcaccact
    41401 gcgggaacgt cttactcccc gtatatcttt atggctttgc cggtgtgtct ggcgtcattt
    41461 ggtttccacg gcaatattcc cagcctgatt atttgctatg gcaaacgcaa agataagtta
    41521 atcaaaagcg tggtatttgg ttcgctgctg gcgctggtga tttatctctt ctggctctat
    41581 tgcacgatgg ggaatatccc gcgagaaagc tttaaggcga taatctcctc aggcggcaac
    41641 gttgattcgc tggtcaaatc gttcctcggc accaaacagc acggcattat cgagttttgc
    41701 ctgctggtgt tctccaactt agctgttgcc agttccttct ttggtgtcac gctggggttg
    41761 ttcgattatc tggcggatct gtttaagatt gataactccc acggcgggcg tttcaaaacc
    41821 gtgctgttaa ccttcctgcc acctgcgttg ttgtatctga tcttcccgaa cggcttcatt
    41881 tacgggatcg gcggtgccgg actgtgtgcc accatctggg cggtcattat ccccgcggtg
    41941 ctggcaatca aagctcgcaa gaagtttccc aatcagatgt tcacggtctg gggcggcaat
    42001 cttattccgg cgattgtcat tctctttggt ataaccgtaa ttttatgctg gttcggtaac
    42061 gtctttaacg tgttacctaa atttggctga tcccttcaag aagccagcca tttcgctggc
    42121 ttattgcctg tcaggaaatc acttatgtcc aaatggcaac tcgcctgatt ctccttcacc
    42181 acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttgatt
    42241 tgtagttttg ccctggtttt actttatccc gccgggattg atatgtacct cgttggttta
    42301 ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tgcatattgc gttctccgtt
    42361 tatctggcgg gcatggcagc tgcgatgtta tttgccggta aagtggccga tcgttcaggg
    42421 agaaagcctg tcgccatacc cggcgcggcg ctatttatta tcgcctcggt gttctgttca
    42481 ctggctgaaa ccagcacgtt atttcttgca gggcgctttc tacaggggct gggcgcgggc
    42541 tgttgttacg tggttgcgtt cgccatttta cgcgacacac tggatgatcg acgtcgggct
    42601 aaagtgctgt cgttactcaa cggcattacc tgcatcattc cggtgttagc gccagtgctc
    42661 ggacacctga ttatgcttaa attcccgtgg cagagtctgt tctggacgat ggcaacgatg
    42721 ggcatcgcgg tactgatgtt gtctttgttt attttaaaag aaacgcgccc tgcggccccc
    42781 acaaattcgg acaaaccacg agaaaatagc gagtcgctgc ttaaccggtt tttcctcagc
    42841 cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ccttcgtcaa cacgtcgccg
    42901 gtattgctga tggaaatcat gggttttgag cgcggagaat acgccaccat tatggcgttg
    42961 accgctggcg tcagcatgac cgtttcattc tccacgccat ttgcgctggg aatttttaag
    43021 ccacgtacgt tgatgatcac ctcgcaggta ttattcctgg cggcgggcat cacccttgcc
    43081 gtttcacctt cccatgcggt ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg
    43141 gtaggttttg gcgtagcgat gagtcaggcg ttagggccgt tttcattacg cgcgggcgta
    43201 gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat ttggctggca
    43261 gcggtggttg gtatcggcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata
    43321 gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc cggttgccgc tcatgaagaa
    43381 atccatcacc acgcttgatc tcaatctgct gctctgtctg caactgctga tgcaggagcg
    43441 cagcgtgacc aaagcggcga agcggatgaa cgtgacccct tcggcggtga gtaagtcgct
    43501 ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg aactcaccgc tggggctgtc
    43561 gcccacaccg ctaatggtca gcatggagca aaatctggcg gagtggatgc aaatgagcaa
    43621 cctgctgctg gataaaccgc atcaccaaac accgcgcggc ctgaagtttg agctggcggc
    43681 ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg aaacggatct accaacgtta
    43741 cccgcaggcg accatcaaat tacgtaactg ggattacgat tccttagatg ccattactcg
    43801 tggtgaagtg gatattggtt tttccggtcg cgaaagtcat ccccgttcgc gggagctgtt
    43861 aagctcgcta ccgttagcca ttgattatga agtgctgttt agtgatgtgc cctgcgtctg
    43921 gttacgcaaa gatcatccgg cactgcatga aacgtggaat ctggacacct ttttacgtta
    43981 tccgcatatc agcatttgct gggaacagag cgatacctgg gcgctggaca atgtgttaca
    44041 ggagctgggg cgcgaacgta cgattgcaat gagcctgccg gaattcgagc agtcactgtt
    44101 tatggcagca cagcccgaca atctgctact ggcgaccgca ccgcgctact gtcagtacta
    44161 caatcaactc caccaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
    44221 aaaaaagctg gaagtccctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
    44281 gatcgtctgg ttacgggaaa ccattaaaca tctttacgcg tcgatggcat aaccgaatcg
    44341 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
    44401 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
    44461 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
    44521 ggaaggacat ctgatttctg ttcgtctccc ctcccattgt catcaagaag cccgaaacat
    44581 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
    44641 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
    44701 tttcagcgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
    44761 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
    44821 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
    44881 cagtaaacaa aatgatccaa acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt
    44941 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
    45001 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
    45061 cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa aagctcagtg tctgggagtt
    45121 agatggcaaa cacggctgga aaagcctgcc cgatattcac agccgcgcca acaatcctac
    45181 cagccggatg atgcgttttg cccagctctc taccgtgaag gctttttcgc caaattgata
    45241 gacaaccaca ggagtcatca tgtctgaaaa attgcaggtg gttacgttac tggggagcct
    45301 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
    45361 catggaagtc aatgcgttac cttccattgc cgacattccc ttgtatgacg ctgacgtaca
    45421 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
    45481 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
    45541 catcgactgg ctttcccgcc tgccggatca acctctggcc ggtaaaccgg tgttgattca
    45601 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
    45661 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
    45721 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
    45781 gcaattaacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
    45841 agcaatcatg catggcgggt ttttaacgcg ctatcgattt tagtgagcgt cgataaacac
    45901 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggcttagat cacgcagacg
    45961 tccggtaccg attttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
    46021 cgagaagctg aacggcatca tcacagcggt aataaacgcc ggaacagatt cagtaagatc
    46081 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaaa tcagcgcgcc
    46141 agctgcggca tatcccggca ccattcccgc cagcggcgac aggaagataa ccagcaggaa
    46201 cagcagaccg acaaccactg ccgtcagacc ggtacgaccg ccaacggaaa cgccggaaga
    46261 ggactcaata tacgccgtaa cagaagaagt cccgataaac gaaccggtca ccgaagagat
    46321 actgtcgaca tacagcgcct gcttcatgcg cgggaattta cctttctcat ccgccagacc
    46381 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaggttga ccagcatgaa
    46441 agagaaaatc acacctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
    46501 gactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtggacat cacccaacat
    46561 ccagcccagc agcgtcgtca ccacgatgga aaccagcacc gccgcgtgaa tgttgcgcga
    46621 ggccagaatg gcgatgatga agaagccgag gatccccagc agcacgctgt gagaagtcag
    46681 attaccgatg ctcaccagcg tttccgggtt agcgacaatc acacctgcgt ttttcagccc
    46741 catcatgcca ataaacagac cgataccact ggtgatgccc acacgtagac ttaccggaat
    46801 gttggctatc atccagtagc gaacgcggaa tatcgtcagt aacagcagac caatcgcacc
    46861 ccagaagatt gcgcccatcc cgacttgcca cggcaagccc atcgcctgta caacgacaaa
    46921 agcgaagaac gcattcaggc ccattgccgg tgccagcgca actggcagat tagcaaacag
    46981 ccccatcata atactgccga atgcagcaat cagacaggta gtaacgaaga cggcgctggt
    47041 atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag
    47101 gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt
    47161 aaacacgcgt tccagcatcc cctggccaga agcctgggtt gtgtgttgtt gactcattat
    47221 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatga gacaatatgg
    47281 acggctgaag ataacttttt ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg
    47341 atttggcatg acgaaaacgt taaactaacg ataaaggaaa gacatgtccc agatagaagc
    47401 ggtatttttc gactgcgacg gtacgctggt cgatagcgag gtcatttgct ctcgcgccta
    47461 tgtcacgatg ttcagggaat ttggcattca cgttgagctg gaagagattt ttacccgttt
    47521 taaaggcgta aaactctacg aaatcatcga catcatcagt aaagaacagg gcgtgacgat
    47581 gatccaggcc gatgccgagc acgtttaccg cgcagaagta gctcgtctgt ttgataccga
    47641 acttgaggtc atcgcaggag cgaacgaatt gctggcgagc attaacgtgc cgatgtgcgt
    47701 tgtctctaat ggcccggtga aaaaaatgca gcattcgctc ggcaaactca acatgctgca
    47761 ctacttcccg gaaaaactgt tcagcggtta cgacattcag cgttggaagc ccgatccggc
    47821 attaatgttc catgcggcaa aagcgatgaa tgtgaacgtt gaaaactgca ttctggtcga
    47881 tgactccagc gccggcgcgc aatcagggat agatgccgga atggaagtgt tttacttctg
    47941 cgctgacccg cacaataaac cgatcgttca cccgaaagtc accaccttta ccgagctggc
    48001 gcagctgcct gcactctgga aggcacgcgg ctggaagata acgcgataat cgcctcctta
    48061 cttcaacgct tacacatcag tcacgctggt ttgagataaa aaataccagg tgtctgatga
    48121 gtaagcgtct attccctata aataatttat gtcgttaatc gtccagtcgc tccccctctg
    48181 tatggttaag cctggtttat atttttcaca atatcgggat accgttcgaa ccaatccaga
    48241 atattaagca tcgcgttact gatattttta ttagccttat attgattctc gcgtctatga
    48301 ttttgaacaa tattaagctt agaacgtatc ttattgtcag agtcagaaat atcctcattt
    48361 aatgccttta cttcatcctg tacttcttga ctattccagt tgtccctttt tttatcaaat
    48421 aaagtccttt tatcatctac tgatttatcc cgacttatgg tcaactcttt aatttctcgc
    48481 aataagtcat cgtaatctgt aatggaagct cttatttcct gatagttttt taataaatcc
    48541 tgcctgttaa ttgaaaatgc actggataaa tattgaaaat cgcgagcaaa cttattgagc
    48601 cttgaatcgt aatctgtctt aaaagatttt atatgttcca gttggtttga tagcgcttca
    48661 atttgttttt tttgaacacc ttcaaaaatg ggtgcaagtt ccttataatc aaatgttaca
    48721 tttgtagcag attctttatg agactccttt atttgctttt ggacatattc ttttggattt
    48781 tcagtaagta ttgttccatc tggcggcgga atcattttat ttattataga ttcaacaaac
    48841 ttaattcg
//
LOCUS       DAYPHN010000015        46203 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000015 DAYPHN010000000
VERSION     DAYPHN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.015"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            339..464
                     /gene="timP"
                     /locus_tag="ACG0BW_000896"
     CDS             339..464
                     /gene="timP"
                     /locus_tag="ACG0BW_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFK7257891.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGNNLLVVNKLQSSK"
     gene            complement(496..1272)
                     /gene="sseB"
                     /locus_tag="ACG0BW_000897"
     CDS             complement(496..1272)
                     /gene="sseB"
                     /locus_tag="ACG0BW_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFK7257892.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQVVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQHRII"
     gene            complement(1414..2697)
                     /gene="pepB"
                     /locus_tag="ACG0BW_000898"
     CDS             complement(1414..2697)
                     /gene="pepB"
                     /locus_tag="ACG0BW_000898"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFK7257893.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2942..3142)
                     /gene="iscX"
                     /locus_tag="ACG0BW_000899"
     CDS             complement(2942..3142)
                     /gene="iscX"
                     /locus_tag="ACG0BW_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFK7257894.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3154..3489)
                     /gene="fdx"
                     /locus_tag="ACG0BW_000900"
     CDS             complement(3154..3489)
                     /gene="fdx"
                     /locus_tag="ACG0BW_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFK7257895.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3491..5341)
                     /gene="hscA"
                     /locus_tag="ACG0BW_000901"
     CDS             complement(3491..5341)
                     /gene="hscA"
                     /locus_tag="ACG0BW_000901"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFK7257896.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRVLKDAGVEADEVMEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5358..5873)
                     /gene="hscB"
                     /locus_tag="ACG0BW_000902"
     CDS             complement(5358..5873)
                     /gene="hscB"
                     /locus_tag="ACG0BW_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFK7257897.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5969..6292)
                     /gene="iscA"
                     /locus_tag="ACG0BW_000903"
     CDS             complement(5969..6292)
                     /gene="iscA"
                     /locus_tag="ACG0BW_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFK7257898.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6309..6695)
                     /gene="iscU"
                     /locus_tag="ACG0BW_000904"
     CDS             complement(6309..6695)
                     /gene="iscU"
                     /locus_tag="ACG0BW_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFK7257899.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6723..7937)
                     /gene="iscS"
                     /locus_tag="ACG0BW_000905"
     CDS             complement(6723..7937)
                     /gene="iscS"
                     /locus_tag="ACG0BW_000905"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFK7257900.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8049..8537)
                     /gene="iscR"
                     /locus_tag="ACG0BW_000906"
     CDS             complement(8049..8537)
                     /gene="iscR"
                     /locus_tag="ACG0BW_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFK7257901.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8807..9547)
                     /gene="trmJ"
                     /locus_tag="ACG0BW_000907"
     CDS             complement(8807..9547)
                     /gene="trmJ"
                     /locus_tag="ACG0BW_000907"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFK7257902.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9666..10469
                     /gene="suhB"
                     /locus_tag="ACG0BW_000908"
     CDS             9666..10469
                     /gene="suhB"
                     /locus_tag="ACG0BW_000908"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFK7257903.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10614..11468
                     /locus_tag="ACG0BW_000909"
     CDS             10614..11468
                     /locus_tag="ACG0BW_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFK7257904.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAENAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11659..12939
                     /gene="csiE"
                     /locus_tag="ACG0BW_000910"
     CDS             11659..12939
                     /gene="csiE"
                     /locus_tag="ACG0BW_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFK7257905.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALTGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKVFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKILESA"
     gene            complement(12931..14070)
                     /locus_tag="ACG0BW_000911"
     CDS             complement(12931..14070)
                     /locus_tag="ACG0BW_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFK7257906.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14230..15120)
                     /gene="hcaR"
                     /locus_tag="ACG0BW_000912"
     CDS             complement(14230..15120)
                     /gene="hcaR"
                     /locus_tag="ACG0BW_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFK7257907.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSITLLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15256..16617
                     /gene="hcaE"
                     /locus_tag="ACG0BW_000913"
     CDS             15256..16617
                     /gene="hcaE"
                     /locus_tag="ACG0BW_000913"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFK7257908.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16614..17132
                     /gene="hcaF"
                     /locus_tag="ACG0BW_000914"
     CDS             16614..17132
                     /gene="hcaF"
                     /locus_tag="ACG0BW_000914"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFK7257909.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGIQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVNETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17132..17452
                     /locus_tag="ACG0BW_000915"
     CDS             17132..17452
                     /locus_tag="ACG0BW_000915"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFK7257910.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17449..18261
                     /gene="hcaB"
                     /locus_tag="ACG0BW_000916"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17449..18261
                     /gene="hcaB"
                     /locus_tag="ACG0BW_000916"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFK7257911.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18271..19473
                     /gene="hcaD"
                     /locus_tag="ACG0BW_000917"
     CDS             18271..19473
                     /gene="hcaD"
                     /locus_tag="ACG0BW_000917"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFK7257912.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKSLGRDTRELVLTNGESWQWD
                     QLFMATGAAARPLPLLDALGERCFTLRHADDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFNAKLLTD
                     EDIALKSL"
     gene            19570..19992
                     /locus_tag="ACG0BW_000918"
     CDS             19570..19992
                     /locus_tag="ACG0BW_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFK7257913.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20039..20911)
                     /locus_tag="ACG0BW_000919"
     CDS             complement(20039..20911)
                     /locus_tag="ACG0BW_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFK7257914.1"
                     /translation="MTIYTLSHGPLKLDVSDRGGVIEGFWRDATPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQSRIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(20923..22017)
                     /locus_tag="ACG0BW_000920"
     CDS             complement(20923..22017)
                     /locus_tag="ACG0BW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFK7257915.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(22050..23048)
                     /locus_tag="ACG0BW_000921"
     CDS             complement(22050..23048)
                     /locus_tag="ACG0BW_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7257916.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23073..24584)
                     /locus_tag="ACG0BW_000922"
     CDS             complement(23073..24584)
                     /locus_tag="ACG0BW_000922"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFK7257917.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24607..25590)
                     /locus_tag="ACG0BW_000923"
     CDS             complement(24607..25590)
                     /locus_tag="ACG0BW_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFK7257918.1"
                     /translation="MPKKMTTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25687..28968)
                     /locus_tag="ACG0BW_000924"
     CDS             complement(25687..28968)
                     /locus_tag="ACG0BW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFK7257919.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSSGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPDRLTIELSDADGNIILSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQGNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESAFAQWRGLCQGRDVNALVTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDQTEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEAAHYESARAELTAINPAWPKAALFTTVMPFI
                     FNRVH"
     gene            29086..30279
                     /locus_tag="ACG0BW_000925"
     CDS             29086..30279
                     /locus_tag="ACG0BW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFK7257920.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQGRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGLLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30477..31730)
                     /gene="glyA"
                     /locus_tag="ACG0BW_000926"
     CDS             complement(30477..31730)
                     /gene="glyA"
                     /locus_tag="ACG0BW_000926"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFK7257921.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32059..33249
                     /gene="hmpA"
                     /locus_tag="ACG0BW_000927"
     CDS             32059..33249
                     /gene="hmpA"
                     /locus_tag="ACG0BW_000927"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFK7257922.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWKGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQALPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33294..33632)
                     /gene="glnB"
                     /locus_tag="ACG0BW_000928"
     CDS             complement(33294..33632)
                     /gene="glnB"
                     /locus_tag="ACG0BW_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFK7257923.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33693..35027)
                     /gene="glrR"
                     /locus_tag="ACG0BW_000929"
     CDS             complement(33693..35027)
                     /gene="glrR"
                     /locus_tag="ACG0BW_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFK7257924.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35017..35730)
                     /gene="qseG"
                     /locus_tag="ACG0BW_000930"
     CDS             complement(35017..35730)
                     /gene="qseG"
                     /locus_tag="ACG0BW_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFK7257925.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAINTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYNDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(35895..37322)
                     /gene="qseE"
                     /locus_tag="ACG0BW_000931"
     CDS             complement(35895..37322)
                     /gene="qseE"
                     /locus_tag="ACG0BW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFK7257926.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLQATACLAEPMLLMSVLDNLYSNAVHYGTESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37898..41785)
                     /gene="purL"
                     /locus_tag="ACG0BW_000932"
                     /gene_synonym="purI"
     CDS             complement(37898..41785)
                     /gene="purL"
                     /locus_tag="ACG0BW_000932"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFK7257927.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAVQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADRKAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42043..43599
                     /gene="mltF"
                     /locus_tag="ACG0BW_000933"
     CDS             42043..43599
                     /gene="mltF"
                     /locus_tag="ACG0BW_000933"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFK7257928.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43596..44099)
                     /gene="tadA"
                     /locus_tag="ACG0BW_000934"
     CDS             complement(43596..44099)
                     /gene="tadA"
                     /locus_tag="ACG0BW_000934"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFK7257929.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KARSSTD"
     gene            complement(44157..44792)
                     /gene="yfhb"
                     /locus_tag="ACG0BW_000935"
     CDS             complement(44157..44792)
                     /gene="yfhb"
                     /locus_tag="ACG0BW_000935"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFK7257930.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45001..45849
                     /locus_tag="ACG0BW_000936"
     CDS             45001..45849
                     /locus_tag="ACG0BW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFK7257931.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            45905..46165
                     /gene="yfhL"
                     /locus_tag="ACG0BW_000937"
     CDS             45905..46165
                     /gene="yfhL"
                     /locus_tag="ACG0BW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFK7257932.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        10939 a        12743 c        11713 g        10808 t
ORIGIN      
        1 tcactttctt taaacaacaa aaaaccgcca aaatcaggcg gttttttgtt gctggtccgg
       61 ttcgcggcct ttccagcagg ttgtattacc gtagtaatgc aagcgcgtct cagcggagac
      121 aatactcgcc agtaaccctc tttttgtcaa gcaaaagaga gttattattg ttctgttagt
      181 gtattatcca ctgcggccct ttccgccgtc tcgcaagcgg gcgctggctt aaggaaagga
      241 tgttccgcga ccgttagagc aagcgcgtct cagcgcttgc tatcgcggca atatcgccag
      301 tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat gaagatacgg tgcttttgta
      361 tcgtacttat tgtttctggt gcgctgttaa ccgaggtaaa taataaccgg agtctctccg
      421 gcaacaattt actggtggtt aacaaacttc agagcagcaa gtaagcccga atgccgccct
      481 ttggggcggc atattttaga ttatccgatg ctgtttaaag tcacgcaaaa aaccacccca
      541 gcgacgttca tagaatggcg caatatgttc ggtaataaag tggctaattc ctttttcccc
      601 ttttttcacc tgacaaatat cgatgggttc atcgccaggt aatgtatcgg tcgctacact
      661 tcccgccgcc tgaataattt cttcgatatc accatcggct tcaatgccaa taagtaaatt
      721 aggctgtgcc tcttcgttct ctttaattga acaaataaaa gcacgcttca ccggcttaat
      781 agttttaaat aaggtggtga gtgaatcaat catttgtgct ggtggctctg cgacttccga
      841 taatatcagc gattcaccgc cctccagtac ttcctggctg ctcagcggat ttccttcttc
      901 accaatcaac aaactgattt cacgcggcat aaattcttta ccggttggca gtttggcatt
      961 aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg cgaacgggca ttacgacaaa
     1021 tgcctgttcg tcttcaacca cctgttgaag tgcttctaac gaggtgaaaa aaggaatgac
     1081 gctggtgccg tcttcttttt cccagtgctg taaatcaagc gcgctatctt caaccacagc
     1141 ctcgccctgc gccgccgtac caggcaccca gacggtggat tccagtagag tacggaaaaa
     1201 ggccgggcgg tgcgccggtt cggttgctgc tttttccagc aggtcttcaa gttcattttt
     1261 tgtttcggac ataagaacca caattcattc aacgttcggg gcacaagtac ctgatacgcc
     1321 acgcttatca ggcctacaag ggagtcgcaa tctattggat tttcgcaatt tgtaggccgg
     1381 ataaggcgtt tacgccgcat ccggcatatt agtttacgcc gttaacagat tagcaatcgt
     1441 gcgcacacca agtcccgtag cacccgcaga ccactgttca accggcgctt tacggtaagt
     1501 cgccgagcag tcgatatgca gccagccttg ctgatagttc tcaacaaagt gcgacaggaa
     1561 gcccgccgcc gtgctcgcgc ctgccgggta cgccgcgctt ccggtattgt tcagttcggc
     1621 aaagttagac ggcagctggc tgcggtggaa ctccgccagc ggcagacgcc agaacggttc
     1681 attctcctgc gcggcactcg ccagcaagcg accggcaagc gcatcgtcaa aactgaacag
     1741 cgcgtgataa tcattaccca gcgcagtttt cgccgccccg gtgagggtcg ccgcatcaat
     1801 gatcagttcc ggtttctgcg cactggcatc aatcaggcca tcggccagca ccagacgccc
     1861 ttcggcatca gtgttcatca cttcaacttt tttaccgttg cgataggtaa taatatcgcc
     1921 cagtttgaac gcgttgccgc tgatcaggtt atccgcacag cagaggaaca gcttcacgcg
     1981 cttgttcagt ccgcgcgtaa tggcaaacgc cagcgccccg gtaaccgttg ccgcgccgcc
     2041 catgtccgac ttcatcgagt ccataaatgc ggtctgtttg atactgtagc cgccggaatc
     2101 aaaggtgatg cctttaccaa ccaggcacgc gtacactggc gcttctttat cgccagtcgg
     2161 gttgtaatcc agcgccagca ataccggaga acgttctgaa ccgcgtccga ccgtgtgcag
     2221 ccccatataa ccttgatcac gcagatcttc gcctttggtg atccggtaag tcacacgatc
     2281 gcccgcgaca ttgctgatca gatcaacagc acgctgtgcc agttgcgatg gccccagttc
     2341 ttccgccggt gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc ggttatccag
     2401 ttcctgccgt tgggcatcgt ccagatccgg ccactccact ttacgtgtgc ctttcggggc
     2461 tttgtaacct tgccagaatg cccagcagcg atccgcatcc cagtcttcac cgcttaactg
     2521 aacatgcttg atgcccagac cgtcaatctt gcgcgccgca cgctggatca gccccagatc
     2581 gtctgccccg ttcaaatgca gggtaatgcc atcattatta atgctgtaag tcgctttttc
     2641 tccccagcgc gcgtcggcag gttgggtaga gagggtaatc ttcatcgctt ctgtcatttt
     2701 agttatcctt ctttgtaaaa gggccgccag ttggcagccc caggattttt ccgctaaatg
     2761 attcgcgttg caggacaaaa cgcattgcgt tttgaacagc gcttgcgctg gccccggagg
     2821 ggtgaggcca aaaggccgaa tcacgcggca agtgagtgaa gccccaggag catagataac
     2881 tatgtgactg gggtgaacga gcgcagccaa cgcatctgcg gcgtgaagca tgacgcggaa
     2941 atcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttt tcgttggatg
     3001 cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata tcggtgaatc
     3061 gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca atttcgcgac
     3121 tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat ggttgatagt
     3181 gtaacgcggg atttcgacca ctaaatcttc gtcggtaacg cgcgcctggc agcttaaacg
     3241 gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct ctgagctttc
     3301 cggcagcgag tcaaaacctt cgcgaacgat gcagtggcag gtggtgcaag cacaggattt
     3361 ttcacaggcg tgttcaatct cgataccgtt acgcagcgca acgtcgagaa tggtttcacc
     3421 actattcgct tccagaacag cgccatcagg gcagagatcc tgatgaggca aaataacaat
     3481 ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga accgactggt
     3541 ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc gcttgttcga
     3601 tagcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca tcgtcaatga
     3661 cctgacgctc tgcggtgctt aacagcgcgg catcagcagc cagcgcgccg tgcaaacttt
     3721 ccagcacacg cgccgcttct actttttgtt ctgccagcat acgggctttt acgtcctgct
     3781 cggcatagct cattgagtct ttaatcatcg aggcgatttc gctgtcggtc agaccgtagg
     3841 acggtttgac ctgaatagat gcctcaacgc cggtggattt ctccatcgcc gtcacgctca
     3901 aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcgccgcca gccggtagcg
     3961 ccggaatacc acgcagcgca aaacgcgcca gcgagcggca gtcctgcacc agttcgcgct
     4021 caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg gtgaaatcct
     4081 gagcgcgcgc caccggaata gtggtattac gcggaatcac tttctccacc aggccgccca
     4141 tcgtttcgag gcccagcgac agcgggatca catccagcaa cagcatttcg ctgtccggct
     4201 tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact ttatccgggt
     4261 cgatagaagt cagcggtgga cgaccgaaaa attcgcctac tcgttcacgc accagcggca
     4321 cgcgagtaga accgccaacc atcaccactt ccatcacttc atcagcctca acgccagcgt
     4381 ctttcagcac acgacgacaa gccagtaagg ttcgttttac cagtggcgcg atcagttcgt
     4441 tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc acggagtccg
     4501 catcgctcag cgcgattttg gccgcaatgg cagcatccag cagttcacgc tgaacgcggt
     4561 tatcgctacg atcaggaatg cccgcctgct cgcgaatgta atccgccagc agatggtcga
     4621 aatcatcgcc gccgagcgcg gaatcaccgc cggtcgccag cacttcgaac acgccgcgac
     4681 ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaacg gcgatcacgc
     4741 cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg ttaagtaagc
     4801 gcaggacgtg aaggcccgcc agacgcgccg cgtctttggt gccctgacgc tgggcatcgt
     4861 caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg gcttcagttg
     4921 cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg caccgggttc agcagccccg
     4981 ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa ggcagatgcg
     5041 gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca gaactaatgg
     5101 tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa tgcccttgct
     5161 gttgatagtg aacaacagac ggcagcaggt gacggccttc atggtcggct aacgtttcgg
     5221 cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca ataccggccg
     5281 ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt aataaggcca
     5341 tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc ggcactgctt
     5401 cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc ccacgtctcg
     5461 ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt cacacgtttg
     5521 ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag ttcttcgcgc
     5581 agttccagct gttccatcag gaacgcggtg tcgcgcacag tatgctgctc gctggcgaga
     5641 tcaaaaccgt gcaaagaaag caaatattcc gcgcgcatta acggatgacg cagcgtttgc
     5701 caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc ctggcttccg
     5761 ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag gctcagcgcc
     5821 tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc cataacattc
     5881 tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc acgcgcaggc
     5941 gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg caaccacact
     6001 catctttgac gttcgggttg gtgaatttaa acccttcgtt caggccttct tttacgaagt
     6061 ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact ttcacgcctt
     6121 tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt acataagcca
     6181 tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg ccgcggttag
     6241 ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac ataaccaaac
     6301 ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc tttgatcgcg
     6361 tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc ttcagcaata
     6421 tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca ttcggtgacc
     6481 agggagctgg aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc gtcttcaatg
     6541 ataccttcat cgttgacttt aatctgcaac ttcatcacgt cgccacaggc cggtgcaccc
     6601 accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt acgcggattc
     6661 tcgtaatggt cgataacttt ttcgctgtaa gccattataa attctcctga ttccgatacc
     6721 gattaatgat gtgcccattc gatgctgttc agatccacgc cctgcttgta catttcccac
     6781 agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat ggtgtagtcg
     6841 atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg tgccagctcg
     6901 tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga cgtacaggcg
     6961 gaacctgaag aaaccgcgag gtctttcagc gccataatca gcgactcacc ttcaacgtag
     7021 ttgaagctga cgttgagaat gttcggcgcg ccgtgttcca ggtcaccgtt caggtaaact
     7081 tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag acgttccatc
     7141 tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccataccaac gatctggtga
     7201 acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat ttgcgcttcg
     7261 atgcgtacgc gcggtttacg acgcacatac agcgcaccga tacctttcgg gccatagatt
     7321 ttgtgaccgg agaatgacat caggtcaact ttcaactggc tcaggtcgat aggcagtttg
     7381 cccacgctct gggttgcatc aacgtgatag ataatgccgc gagcacggca catttcgccg
     7441 atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat ggaaacgagg
     7501 atggtgtcgt cacgcatcgc tgcttccagt tctttcaggt cgataatgcc attacgctgc
     7561 ggtgccaggt aggtgacttc gaaaccttcg cgctccagct gacggcaggt atccagtacc
     7621 gctttgtgtt cggttttact ggtgatgatg tgcttgcctt ttttctgata aaagttggct
     7681 gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac gatttcacgc
     7741 gggtcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc ttcttcagcc
     7801 tgccagccga aacggtgaga acgggaggcc gggttaccaa aggttccgtc catcgtcata
     7861 aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga gtagtcgaga
     7921 taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa ggaaccaggc
     7981 taccggctgg atgtacgacc gtgtttacgg agcgcgatac gccccggcct gattctgaat
     8041 tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt gcgtggcgcg
     8101 tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac cagttcgcct
     8161 aaagtaatgt tgttgagaaa accggtgaga cggtcgctca aatcacgcca tagcgcgtgg
     8221 gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt ggcatctaca
     8281 gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc tttgcctaac
     8341 agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg cagacgggaa
     8401 aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc agccaacggt
     8461 accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc atagcgccct
     8521 ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg ggttttttat
     8581 tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt aaattggtca
     8641 actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt tttatcgtgg
     8701 cgtttgtacc tgttgtcgga tgcgacgtga acgccttatc cgacctgagg ctctgtgcac
     8761 tgtaggcctg atacgacgcg ttaagcgtcg catcaggtaa ccactattat tcggccttat
     8821 tacctttatt ctgctgctct atagaagcca gaatcccgcg caggatattc aattcctggc
     8881 tttccgggcg cgcacgggta aacaaacgac gcaatttatt catcacctgc cccggatggt
     8941 tttcacgaat aaaaccggtt gccagcagcg tttgttcgag atgaccataa aaacgctcca
     9001 gatcatcgac cagcggatac ggcgtctctt catgctcaac ctgctcgcca ttttcctgag
     9061 ttgccagcca ggccatgcgc acttcatagg caataacctg aaccgccatc gccaggttca
     9121 gcgaactgta ttccgggtta gccgcaatgg cgacatgata atggcatttc tgcaactctt
     9181 cattggtcaa gccgacgcgc tcgcgaccaa aaaccagcgc caccggggta tttgccgctt
     9241 cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc agcgtgcggg
     9301 agcgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg agcgtatcga
     9361 cgatatgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc gcctgggagt
     9421 caggtttcac cagtggatta accaaccaca gattggttaa tcccattgtt ttcatggcac
     9481 gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt cgaatatttt
     9541 gcagcattgt ctttcttcgt ctaaagatta ttcgcgcatc ttatcataaa acgaagacag
     9601 atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga
     9661 gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa
     9721 ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc agtaacgact
     9781 tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt cgtaaatctt
     9841 acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact gatcaggatg
     9901 ttcaatgggt tatcgatcca ctggatggca ctaccaactt tatcaaacgt ctgccgcact
     9961 tcgcggtatc tatcgccgtt cgtatcaaag gccgcactga agttgctgtg gtttacgatc
    10021 ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg aacggctacc
    10081 gtctgcgcgg cagcaccgct cgcgatctcg acggtactat tctggcgacc ggcttcccgt
    10141 tcaaagcaaa acagtacgcc accacctaca tcaacatcgt cggcaaactg ttcaacgaat
    10201 gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtt gctgcgggtc
    10261 gcgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgccgca ggcgagctgc
    10321 tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac tacatgctga
    10381 ccggtaacat cgttgctggt aacccgcgcg ttgttaaagc catgctggcg aacatgcgtg
    10441 acgagttaag cgacgccctg aagcgttaat gactctggcg ggtgatgtca ctcacccgcc
    10501 ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct ttcgccactc
    10561 tcccaccatc tttccccgat taatggataa taaagaagtc gcaaggaaag gttatggcgc
    10621 tgccagtgaa caaacgcgtt ctcaaaattc tgtttattct ctttgttgtt gccttctgca
    10681 tttatttaat accgcgcatc gccatcaatt ttttctatta ccctgacgac aaaatctacg
    10741 gccccgatcc ctggtcagcc gaatcagtag aattcaccgc taaagatggc actcgcctac
    10801 atggttggtt tatcccttct gcaactggcc ccgctgaaaa tgccattgcc accattattc
    10861 atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt tggttacccg
    10921 agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca aaaggcacgc
    10981 cgtcccaggc cggattgctg gacgatacgc aaagtgccat caatgtggtg cgccatcgca
    11041 gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg gcgaatattc
    11101 tggatgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc gactccacat
    11161 tcgcctctta tgcgaccatc gccaaccaga tgctccccgg cagtggctac ttacttgatg
    11221 agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt ttactgattc
    11281 acggtaaagc tgatcacgtt atcccatggc agcacagcga aaagttgtat agcctggcaa
    11341 aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct ttttccgatc
    11401 gtcacggcga tgtttatcgc gaacagatgg tggattttat ccttagcgcg ttgaatccgc
    11461 agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta agacatgcta
    11521 atccactggt cagaacagtt taagatgaga aaaattctgt gacgcttgcc aacatttctg
    11581 atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt atcaattatg
    11641 cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct ttcggctccc
    11701 cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc caccacggca
    11761 actttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat cagcgaaaca
    11821 ggacgggaga tcctgcgcta tcatcaactc acactgacta ctggttatga cggtagctac
    11881 cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct acgacgtggt
    11941 ttccgtctgt gtccgtcatt tattaccagc catttcaccc ccgccctgaa gagtgaactg
    12001 aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc gttagtgaat
    12061 ctctgctccc gacggctgca aaaacacttt gaaacgcgcg atattcattt cctgtgtctg
    12121 tatctgcaat attgtttgct gcaacaccac gctggaatta cgcctcagtt taatccgctc
    12181 caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat tggacgccac
    12241 tggcagcgtc gagcgctcca gcctgtaccg cctgatgagc cactgttcat ggcattactt
    12301 ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga cagacaactg
    12361 cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat
    12421 gatgaacagg ggttgtgcga tcagctttat acccacctcg cccaggcgtt aaatcgcagt
    12481 ttgtttgcca tcggtattga taacaccctg ccggaagagt tcgcccgcct gtatccacgc
    12541 ctggtgcgca ccacccgcgc ggcgctgacc ggatttgaaa gtgaatacgg cgtccatctt
    12601 tctgatgagg aaagcggtct ggtcgcggtg atttttggtg cctggctaat gcaggaaaac
    12661 gacctgcatg agaaacagat tattctactg accgggaatg atagtgagcg agaagcgcag
    12721 attgagcagc agctacgcga actaacgtta ctgccgctca atattaaaca tatgtcggta
    12781 aaggtatttt tgcagacagg cgctccgcgc ggcgcggcac tgattattgc tccttatacc
    12841 atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgct gacaacacat
    12901 caacaggagc agatccgcaa aatactcgaa tcagcatgag ggaacaactt tcgggcgcaa
    12961 aaacattgcc ggaagcgcca ccagcgccat cacccagaac acgccgtggc ccagatattg
    13021 atacaggaaa ccggcgaaaa cggtcatgat ggcgatactg ccgcccatcg cgacggcaga
    13081 gtaaaccgcc tgtaaacgga tgacttcgct accctggcga gcggcaatat agcgcatggc
    13141 ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta tcaaccacgg
    13201 caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg ccgagatcaa
    13261 cagcatatcg cgtgcactac aacggcggaa aagtttatta ctcagcgcaa agataatgac
    13321 ttccgccacc acgcccagcg accacaaata gcccaccgcc gaggccgagt agccagcggc
    13381 ctgccagtaa atggcgctaa aaccgtaata ggccgcatgc gccccctgca ataaacaaac
    13441 gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc aaccggtgct
    13501 ctcctgctgg cggcttgccc cttgtggctg aatcgttgga cggatgagaa agccgagcag
    13561 catggatgcc acgcccaacg tcaacagcgc gaggatcacc cgataatcaa acatagtgac
    13621 cagtttaccc gtcaacgccg agccaatgac aaacgccacc gagccccaca atcgcacttt
    13681 gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg tcaacggtac
    13741 cagcggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg ctacgtgcgc
    13801 ccccgcccag aaggcgactg caaagagaag tgtcagcagt gccagcacgc gcaaggcgga
    13861 aatcaggcga gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga gaaaacgggc
    13921 aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa tccctttaag
    13981 ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa aagtatgtga aatagccgag
    14041 cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg cgttagtctg
    14101 gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg cttcccgatg
    14161 tctgtaacgc cagttcacaa gcactcaaaa actgcgacct gcgacgcatg ttgcaaaaat
    14221 aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga tgaaatcgcg
    14281 taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagtaatgg aaggcacatt
    14341 tccggcaatg ggtcgaaata ccacctggcc ggtattaaaa ttattcatat aaccgggtat
    14401 caaagtgacg cccagcccca tccccaccag attcatggtc accagaatat tcgttgccac
    14461 ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga tcggcgcaag
    14521 cgaaccggaa tacgctggat cagtactgac gaaattcacg ccatccagtt gcgcggcagt
    14581 gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta atggttcgtc
    14641 aaaaagctcc agataatcaa tctccgggct ataaacagga tggcgcatta agccgacgtc
    14701 gagttcacca cggcgaattt tttcttcctg ttccgtggtg attaaactca ccagctcaat
    14761 taaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcagta aattcacttc
    14821 cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt tccgcgcccg
    14881 taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa aacattctcc
    14941 cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac cgacacagtt
    15001 ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt tttccgccgc
    15061 acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc gtagttccat
    15121 caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta tttcacatat
    15181 tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac atcttatctt
    15241 tcaggattaa aaaatatgac caccccctca gatttgaaca tttaccaact gattgacacc
    15301 caaaacggtc gggtcactcc gcgtatttat accgatccgg atatttacca actggagctt
    15361 gagcgcattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat cccaaaaccc
    15421 ggtgatttct ttaacaccta catgggagaa gatgcggttg tcgtggtgcg ccagaaagac
    15481 ggcagcatca aggcgtttct caaccaatgc cgccatcggg ccatgcgtgt gagttatgca
    15541 gattgcggca acactcgcgc ctttacctgc ccgtatcacg gctggtctta cggtattaac
    15601 ggcgaattga tcgacgtacc gctggaacct cgcgcctacc cacaagggtt gtgtaaatcc
    15661 cactgggggc taaacgaagt tccttgtgtg gagagttata aagggctgat ttttggcaac
    15721 tgggatacca gcgcaccggg tctgcgtgat taccttggtg acattgcctg gtatctggat
    15781 ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca aaagtgggtg
    15841 atcaactgca actggaaatt cccggcagag cagttcgcca gtgaccagta tcatgctctg
    15901 ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag cgataagcgt
    15961 ctcggtgatg gacaaactgc ccgcccggtg tgggaaaccg ccaaagatgc gctgcagttt
    16021 ggtcaggacg gtcacggcag cggttttttc tttactgaaa aaccggatgc taatgtctgg
    16081 gtcgatggcg cggtttccag ctattaccgc gaaacctatg ccgaagcaga acaacgttta
    16141 ggtgaagttc gcgccctgcg cctggcgggt cataacaata ttttccccac gctttcctgg
    16201 ctcaacggca ctgccacgct ccgcgtctgg catccgcgcg gccctgatca agttgaagtg
    16261 tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc ttttgaaaac
    16321 agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc ggagaactgg
    16381 tgtgaaatcc agaaattgct gaaaggtcac cgcgcccgca acagcaaact gtgtctggaa
    16441 atggggcttg gtcaggaaaa gcgtcgcgac gacggcattc caggcattac taactatatc
    16501 ttctcagaaa cggccgctcg tggaatgtac caacgctggg ccgatctcct gagtagcgaa
    16561 agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat gaaatgagtg
    16621 cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac gaagccagtc
    16681 tgctggatga ctggaaattt cgtgactggc tggcacagct cgacgaagag attcgttaca
    16741 ccatgcgcac cacagttaac gcgcaaacgc gcgaccgccg caaaggcatt cagccgccga
    16801 caacctggat ttttaatgac accaaagacc agctggaacg gcgaatcgcc cgtctggaaa
    16861 cgggtatggc ctgggcagaa gaaccgccgt cacgcacccg tcacttaatc agcaactgcc
    16921 aggtaaacga aaccgacata ccaaacgtat ttgctgtgcg ggtaaattat ctgctttatc
    16981 gggcacaaaa agagcgcgat gaaacattct atgtcgggac tcgttttgac aaagttcgcc
    17041 gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctggat caagcggtga
    17101 tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt gtcccgttgc
    17161 ggatgtgccg gagggtgagg ctctccggat cgatacctcg cccgtcatcg ccttgttcaa
    17221 cgtgggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg cgtcgatgtc
    17281 agaagggtat ctggaagatg acgccacggt ggagtgcccg ctgcacgccg ccagtttttg
    17341 cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctaacca cttatccagt
    17401 acacgttgaa ggtggtgaca tcttcatcga cttaccggag gcgcagccat gagcgatctg
    17461 cataacgagt ccatctttat taccggcggc ggatcgggat taggcctggc gctggtcgag
    17521 cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc aaaagtcgcc
    17581 agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt gacctgctat
    17641 gccgattatc aacgcgcggt taatcagatc ctgactcgtt ctggcaagct ggattgtttt
    17701 atcggcaatg cgggcatctg ggatcacaat gcctcactgg ttaacactcc cgcagagacg
    17761 ctcgaaaccg gcttccacga gctgtttaat gtcaacgtac tcggttacct gctgggcgca
    17821 aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac actgtcaaac
    17881 gccgcctggt atcccggcgg cggtggcccg ctgtacaccg ccagtaaaca tgccgcaacc
    17941 ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa tggcgtcggc
    18001 ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgggcaaag tgaaacctcg
    18061 ataatgcagt ctctgacgcc ggagaaaatt gccgccatat taccgctgca atttttcccg
    18121 caaccggcgg attttacggg tccgtatgtg atgttggcat cgcggcgcaa taatcgcgca
    18181 ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat tcgccacgta
    18241 gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaacgatcat tattgtcggt
    18301 ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt caccggtgag
    18361 ctgcatctgt tttccgatga gcgacatcta ccttatgaac gccccccgct ctcgaaatcc
    18421 atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg gtggcaggaa
    18481 aacaatgttc atctgcattc cggtgtaacc atcaaatcgc tgggccgcga cacaagagag
    18541 ttagtgttaa ccaacggcga aagttggcag tgggatcagc tgtttatggc aaccggcgcg
    18601 gcagcccgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac cctgcgccat
    18661 gccgacgatg ccgccagact gagagaagtt ctgcagcccg aacggtcagt cgtgattgtc
    18721 ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgtgg atgtaaggtg
    18781 acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc cgtgcaacgc
    18841 tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa tgccattgaa
    18901 catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggcgaaac gcttcaggcc
    18961 gatgtggtga tttacggtat tggtatcagc gccaacgacc aactggctcg cgaggccaac
    19021 cttgatactg ccaatggcat tgtcattgat gaggcttgcc gcacctgcga tcccgcgatc
    19081 tttgccggtg gcgatgtggc aatcacccgt cttgataatg gtgcactaca ccgctgcgaa
    19141 agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt ggggctaccg
    19201 cttccgcgac tgccgccgcc gtggttctgg agcgatcagt acagtgataa cttacagttt
    19261 attggcgata tgcatggcga tgactggatt tgtcgtggca acccggaaac tcagaaggcg
    19321 atttggttta atctgcaaaa cggcgtgctt atcggtgcag tcacgctgaa tcagggccgt
    19381 gagattcgcc caatccgcaa atggatccag agcggcaaaa cgtttaatgc gaaactgctg
    19441 acagatgagg acatcgcgct taaatcactg taaccaggat aattagcgaa tatctcaacg
    19501 cctggggcgt ggcgaggtac cagagtgtgt attacgttta aatcacatta tcttgcaaag
    19561 ggattggtta tgaactcact acgttatttt gattttggtg ctgcacgccc cgttttgtta
    19621 ttaattgccc gtatcgccgt ggtcttgatt ttcattattt ttggttttcc caaaatgatg
    19681 ggctttgacg gtacggtcca atatatggcc tcgctgggcg cgccaatgcc aatgctggca
    19741 gcgattattg cggtagttat ggaagtgccc gccgcaatat taatcgtgct tggctttttc
    19801 acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacggcggt aattggtcac
    19861 cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt ctggaagaat
    19921 gtcagtatcg ctggcgcatt cctgctgttg gcaattaccg ggccgggggc aatttctctc
    19981 gatcggcgtt aaaaacatgc cggatgcggc gtgaacgcct tatccggcat tttataaatt
    20041 acagtaacgc aacccgcaac gacatctctg aagttgttga ttcccctggt gcaagcgcaa
    20101 tgagatcacc cccttccggg cgatgatggt catctggcgc atggctcatc ggttccagac
    20161 agaagaaatc aaacgcatat cctttatcaa acgccgggtc ggaaacaaag atgaaataac
    20221 atggtgcagg tggcgtcgtt tccatgatga tggcgtaccc ctcctgcggc tgctcgatgc
    20281 gggactgacc attccatccg gcaaagccat tgtttaccca ctggcgcggc aacggcgcga
    20341 gctggttgaa atccagctcc tgcggtagct gctcgcaaaa ctcacccgcc agccactgct
    20401 cccgctccag ccagtaaccg ctcgcctgcg cctgaatccg cgtttgtggt gacaacggaa
    20461 aataaggatg ccagccggta ccaaatggca gcgtctctgc cccttgattg gtgacagaga
    20521 gcgtcaccgt cagcgtatct gccgttaaat gaaacgcctg acttacccga tagtgataaa
    20581 caccgctgcg atgttcatac accagacaca agctatcttc gctgcgcgag acacattgcc
    20641 actgccccag ccagccgtcg ccgtgcagat agtgcgcatc ccactcaaca ttcggttgca
    20701 gttgatactc acgcccctgc cagacaaaac ggttgccact cacccggttt gcaaacggca
    20761 ccagagggaa acaagaggca tcggttgcca caccgctttt tttaccggga cgtaagagcg
    20821 gcgtcgcgtc acgccagaag ccttcgatga cgccgccccg gtcagaaacg tccagcttta
    20881 gcggcccgtg agataacgta tagatggtca tcagctgact ccttaatccg ggaagttaat
    20941 cacaactttc ccgcatttac cgctcgccat cagcgcatag gcatctcctg cctgttccag
    21001 cgtgaagcga tgggtaatgg cgttacgcgg ccacagtttc cagtccgtta aatcgtgggc
    21061 gcatttttcc atatggaaca gactggtcac ccaggagcca ataatccgcc gttgatgatg
    21121 catcagatca gcgctaacct cgaactccac ttttccggtt tctccgatgt aaaccacccg
    21181 tccccagtca gcagtggatt gcaacgccag taagcgacct gcggcattac cggaacaatc
    21241 gagcgcaaca tccgcgccac cgtgggtaag ttcggcgata atctgcggca gaccttcggt
    21301 cgttgctaaa tagccatgat ccatcacccc taactgtttc gccatcgcca gacgttccgg
    21361 cagcatatca acgccgatga tcctttttgc accgcgacct ttcgccagca tcatcgccat
    21421 catgccgacc ggccccagac cgaccaccag cacgttatca ctgccggaaa cttcgccgcg
    21481 caaaatccct tcatacgctg taccaacgcc gcaactgata aacgcaccat cttcatagct
    21541 cagcgcgtcc gggagcagga tcagatcttt ttcttccgcc agcaggtatt cagcatggcc
    21601 gccgtcacgc tgccagccgt aagccgcttt tccttcgcca gtacaagaga taggaaaacc
    21661 acgacggcag ttcgggcaaa aaccacagcc agaaatatga tacaccagca cacggtcgcc
    21721 ctctttaaaa tggcggcagc cttgccccat cgccacaatt tgcccgcacg gttcatgacc
    21781 gttgataaat ccctggtata acggtttatc gggtgccgcc gccgtagcgc ggtgttggtg
    21841 gtagatatag tggacatcgc ttccgcaaat tccggaggat ttcattttga tcagtacctg
    21901 gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg acggtcgaat ttcctggtaa
    21961 ataagctgcc agcatcgttt tcataaatcc ctcattaaca atacgattaa ttttcatccc
    22021 tgccgcaccc gcgccaggga cgtttaggtt tagcgtttcg ctttactgct tcgctgagtc
    22081 agcaagatat tcgccagcac cgccaccacg atgatgacgc cgcgcaccac ctgctggaaa
    22141 aaggagttaa tcccgagcag caccagaccg ttaccgatta gcgtaatcac caacacacca
    22201 agcaatgtac cgaacaggga accgcgacca ccggaaagcg ccgtaccgcc aaccacgacc
    22261 gcggcgatga cgtcaaactc cagaccgttt gcggcacctg cgttaccgga accgaggcgc
    22321 gccgccaaca aaatgccggt caccgctgct aataatcccg aaagggtaaa aataagaatg
    22381 cgtacccgac gaacgttaat accgcataac tgcgccgccg tggcattacc gccaacggca
    22441 aaaaccgagc gcccgaaggc ggttttgcgg ctaatgaaca caaacagcgc aaacagcacc
    22501 atcatgatca gcgcggatac cggcacaccg agaaattgtc cgcccagcca gtccagcact
    22561 tcgttttcgt caatcggcac tggcaaggcg ttcgtcataa atagccccat tccgcgcagg
    22621 gcgctccata aacccagcgt ggcaacaaaa cttggcacgt taaacacgcc gcgcagcacc
    22681 ccggcgagcg ttcccatcag cgcgcctaac agcaacacca gcagacaagc aatcgccagc
    22741 ggaacctcaa attgcagcaa aaatgccagg cacaccgaga caaaagccac catcggccca
    22801 acgctgacat caatttcccc ggagataata atcagcgtca tcgcccaggc ggcgatccca
    22861 atggtggcgg catcgcgcag cacgttcatc tggttattca atgagataaa gccaggcgcg
    22921 ttcagagaga agaccagata aagaatggcg atcaccacca gcaaaccgat ctcattaata
    22981 tggcgactga caaattgttt gagcgagacg ctcttgccct gcggcaatgg taatgacgaa
    23041 gcagacatct tcaggttcct cggtaaaatt catcagtgca cagacagaat ggcggacatc
    23101 agctcatcca cattgaccgg cgagtgaaac tcctgcgaga acgtaccgtg ctgtaataac
    23161 aggatgcggt cacacaccag cggcaactcc tccacttcac tggagataaa caccacgctt
    23221 tttccttcgg cagccagctc gcggacaata cggtaaatct gctgtttggc ttcgatatcg
    23281 acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg cagcatagac ccaacgaccg
    23341 atcaccactt tttgctgatt gccaccagaa agcgtgccga tgggcgtttc gctactggcg
    23401 gccttgaccg tcatccgctg catcacctct tcggtcaggc ggcggatggt ggaccattgc
    23461 agcacaccgt tggcgctgat tttttgccga ttggtcagca ctgtattttc gtcaacgccc
    23521 agccagggaa tgatccccgc ttctttgcgg ttttctggcg tatagccaat gccgcgtttc
    23581 agcatgtcgc cgtaatcggg gcgagtgatt ttctcgccgt tgataacaat ttcgccctgt
    23641 tcatacgtct ccagcccaac tatggctttc aacaattcac tgcgccctgc ccccagcagg
    23701 ccagcaatgc cgagtacttc gccacgacgg agcgtaaagc tgatatcctc cagcttgggc
    23761 ttatggcgta acgcacggac ttccagcacg gcctgatcga caatttcctg aggggaaacc
    23821 ggcgcaatat caacgtgatc gcgcccgagc attagcgaca caatatgatg cgtggaggtg
    23881 ttttcgagca tcacatcgcc cgccacctga ccatcgcgca taacggtggc acaggaggca
    23941 atgcggcgga tttcttccat ccggtggctg acataaatca ccgccacgcc cagtgccgac
    24001 atctttttaa ccgcgctgat caccagttca acttccgcac tggcaagcga actggtaggt
    24061 tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc gcgcaatttc caccagttgc
    24121 ttttgcgccg ggcttagcgt tgaaacaagt tgttcagggc taacgtcaac gcccagcgcc
    24181 tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat caatcatgcc gttgcggcgg
    24241 ggccactgac cgaggcagag gttttccgcc accgtcagtc cctccaccag acttaactcc
    24301 tgataaaccg cacgaacccc cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct
    24361 tccagtcgcg tctcgccaat ccagatatca ccgctatccg gacgttcact gccggtaagc
    24421 attcgaatga gggtcgattt gcccgcgccg tttttgccta acagcgcacg aacttcgcct
    24481 ttattgagcg tgaagttaac gttatccaac gcaacgacgc cgggataacg cttatttcct
    24541 gccaccactt ttgctaccgg gactgcctct gttgccgtga acatagggac ctctgcgaat
    24601 cagcgattag ggcagaccat caacgtgcgt tgccagccac tgtttaccgt cttccgtttt
    24661 ggtatagaga tcgataggca cctgaatcac tttttcaccg tcggcttgtt tgttgataac
    24721 cttcaatgtt tgcgcgaaga cagcattgcc cattttctta ccggaaatat ccaccaccgc
    24781 tttcaacacc tgattgtttt ccagctcctg agcaatttca gtggtcatat ccgaaccgaa
    24841 aacagcaatt tttccggcct gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc
    24901 accacccgat tctcccataa tggcgttgag atccggcgtg gagataatta gtttttcacc
    24961 aacggaaatc gctttatcta aaacagtccc ttcctgatta gcgacaattt gcgcgccggg
    25021 aacgcgggat tttaatactt cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc
    25081 ttcgcaattg atgacggcaa ttttcggctg gtcaatttta ttggcaataa aataatcggc
    25141 ggcagcgtta cccagttttt taccaaattc cagcggatcg ccgaccagat acgccgagac
    25201 atatttatcg acgccttttt gattaataca ggtgttgtag caaatcaccg gaatacccgc
    25261 ttcactggcg cgacgaacgg tacggctact gccattttca gacactgccg ataaaataat
    25321 ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta ctttctttcg aaatatcacc
    25381 ctgtgcgtta gtttcaatta actgtacctg tactgaagaa tcttttgccg catcctgaac
    25441 gccctgacgc acgccagcgt aatatccctg agtatcaagg tatattgcgc caatggtcat
    25501 ttctttatcc gcagctctgg caaatagtgc gctacctaat agcgtagcca ttaataatag
    25561 attacgggtt gttgtcattt ttttaggcat agtgcctcct gtagggtttt tatttacaac
    25621 ggcttattct aattattttg tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa
    25681 tagggtttag tgaacgcggt taaaaataaa aggcatcaca gtggtgaata atgccgcttt
    25741 cggccaggcc ggattaatcg ccgtcaattc agcgcgagca gattcatagt gcgcagcctc
    25801 cccgagtccc gcggacgcca gcatcgccac catcaggcat ttttctttat gctgctgttg
    25861 taaatcgcca tacagcgaca acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat
    25921 actggttttc gccatctctt gcgcccactg tttcatttca ctaaacagtt gctgtgcggt
    25981 ttgctgttca cccagcagtc gcagcgccat tccttgccag aagagataat caaccggctg
    26041 atcgttgtaa taactgtgaa tgttaatggt gcgatcgcca gtcgccgcca gacgtaaaca
    26101 acgcatcgct tcagtttgat cgccctgcgc gttggcgcat atcgcctgcc agaaccagat
    26161 gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc
    26221 ggcatgaagc agttcgctgg cctgctgcgg ctctctggca tcaagatgct gccaggcgcg
    26281 taataactgg ttgaggataa actgactggt gatcttccct tccccgcctt cccacgggtg
    26341 gaatttgcgc gtggcgagaa tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa
    26401 attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca atttccagat tattttccag
    26461 acgcgccagt cgtttctccg gtgtggttcc acttaactta tccagcaaat cccgttcgaa
    26521 aagcagacgt gcatcctgcg gcgcaagctg ataagcatta tcaagataac gcgcggccag
    26581 ctcataatcg tgttgcttat tccacgcatg gatcgccaac ccgcgccagc catcggcaaa
    26641 ctctggcgac atctctacgc aacgttgcca taaagtaatg gctttgttgt agctgcgctt
    26701 gttgtagtag aagcaggcca gtaaatgacg ggcaaaccag cactcttcaa tactctccag
    26761 cgccgccact tcttccagcg tattcgggaa acggacaaac tgtgggaaga catcaatggc
    26821 ttttgcgacc agttcgccgc gttcagcttt cggcagcaag ctggcttgca ggtaaagcgg
    26881 cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc gccagggtgg gcattcccca
    26941 gttaatcagt tgcccggcgg tcaccagggc gttaacgtcg cgtccctgac acagaccgcg
    27001 ccactgcgcg aaggctgatt cgctacgacc atcgaaccag ttcagccacc acagagtggc
    27061 gttcagcgga taatcacgca gcagtttctc acgctgcaaa cgcgcgttgc cctgacgacc
    27121 acttaacacc agcagcagat tatgcaggca aagcacttcc tgattggttg ggcaggcgcg
    27181 aagactttgt tggcaaaaat ccagaccagc gtcgaagtta ccattacgcg ccgccagccg
    27241 tgccagacca taatagccac ctgctttact gttgccgctc cagaccgcac gccagaaatc
    27301 ctcttcggct tgttgatatt gcccctgacg ttcgtaagca ctggcgcgaa tcaaactcgc
    27361 ctgtccgcac tgcggatttt tgttcagcgc atgtgcgcgt ttcagcgcct gagtggcata
    27421 cgccaccgct tgcgggaaat ctgcgcggtt atattccagc atcgccagcg ccaggttaca
    27481 gcgataatcc agcggatcca gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct
    27541 ggcgtgatga tattgctcca gatgctgacc gataaaccag gcttcatctg tactggtaat
    27601 gtcttgtgct gccagtggcg ctttggcgac gtccggcaac ggcaactctt gcggctgatg
    27661 ttcctgataa ctcagtatga tattgccgtc ggcatcggag agctcaatgg tcagtctgtc
    27721 aggattgata ccgtgcaaca cgccctggat ggcggtcgct ggcatcagtg ccacggcatc
    27781 atcaagtaac gcgttgcatt tgccgatttc gcggatcgcc aggcggtatc cgttcaacgg
    27841 agagatggca tacagccccc actcaatccc ccgctcacta cgctggagtt ttatcaccgc
    27901 atcgcgggag gcattttgca ccatgcccag agaatgataa ggcaggaaat actgctcgaa
    27961 acgcttctct tcgtacgcat caagccaggt aaaatcaggc tggttatcgg caaaaatacc
    28021 ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga ctcttatccc acgcctggcc
    28081 aaactcactg tgtccccaac tccactgttt tttacctggc gcaatatggt ggttggcaac
    28141 gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg acaaaatcgt actgcgattt
    28201 ttcagccata tatgaggttg gaacgggcac atttttatag cgagaaatgt ccactccaga
    28261 ggagtagtcc actttgtagt aagtgccggt ggcgatgggg aaagcggaga cggcccgttt
    28321 gccgtgatca aagaccgccg ttacatccgg cgggaagacg ctctgatgcc cttcaccccc
    28381 tttcactgcc gggttggccc accacaagaa atgacgcgga gtggcgttgc cgttatagac
    28441 gcggctggcg atttccagag ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg
    28501 taaaccgtgc atcggctccg tttcgccgac ccacacggtt tgtgcgccgt cttcatgggc
    28561 ttcgagggtg aaatcgacgg gcataaaggt ggtcgggcga tggtgttgcg gccagttaaa
    28621 ctcaatcccg ccggagatcc acggtcccag cagccccacc agcgcaggtt taatgacttc
    28681 attgtgatag acaaaatcgc gctgtttcac tttatcccat gcgcgatgca cccgaccgcc
    28741 cagttccggc aggatcatca ctttaatgta gtcgttttcc agccacaccg cctgccagga
    28801 tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga
    28861 cgatccctga taaacgcgat tttccaggaa catgggatgt atatcctgcg gcccggtttc
    28921 ataggtcggg atctcaacgc gctcttgcca cacttttact ggagtcatgg tgccctcaat
    28981 attaacaaga catactgaat taaaagattt gtggcagtgt attgaacaat ctggcaatgt
    29041 tttcgcggaa taatcacgca attaactaaa caaggtttag taaagatgag agcctgcatt
    29101 aacaatcaac agattcgcca ccataacaaa tgcgtgattc tggaactgct gtaccggcaa
    29161 aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa tttcgattcc ggcggtcagt
    29221 aatattttgc aggaactgga aagcgaaaaa cgggtggtga atatcgacga tgaaagccag
    29281 acgcgcgggc atagtagcgg tacatggctg attgcgccgg aaggtgactg gacgctgtgc
    29341 ctgaacgtga cgcccaccag tattgagtgt caggttgcta atgcttgttt aagtccgaaa
    29401 ggtgaatttg agtatttaca gattgatgca ccgacgccgc aggcgctgct gtccgaaatc
    29461 gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc gtaccatcaa cctggcgctg
    29521 gcaatccacg gtcaggttga tccggtgact ggcgtgtcgc aaaccatgcc gcaagcgccg
    29581 tggacaacgc cggttgaggt aaaatatctg ctggaagaga agctcggcat tcgggtgatg
    29641 gtcgataatg actgcgtgat gctggcgctg gcggagaaat ggcaaaataa ttcgcagggc
    29701 cgggatttct gcgtgatcaa cgttgattac ggcattggct cgtcgttcgt gattaacgag
    29761 caaatttatc gcggcagttt gtatggtagc ggacaaattg gtcacaccat cgttaatccg
    29821 gatggcgtcg tctgcgactg cggacgttat ggctgcctgg aaaccgtcgc ctcgttaagc
    29881 gcgttaaaaa aacaggcgcg ggtatggcta aaatcacaac cggttaatac tcagcttgat
    29941 cctgaaaaac tgactaccgc gcagttaatc gctgcctggc agagtggaga accgtggatc
    30001 accagttggg ttgatcgctc tgccaacgcc attggtttga gtctgtataa cttcctcaac
    30061 atcctcaata ttaatcagat ttggttgtac ggtcgcagtt gtgcctttgg tgagcactgg
    30121 cttaatacca ttattcgcca gacgggattt aacccgttcg accgcgatga aggaccgagc
    30181 gtgaaagcaa cgcaaattgg ctttgggcta ttaagccgcg cacaacaggt gctggggatt
    30241 ggctatttgt atgttgaggc acagttacga cagatttgat ggcgcgataa cgtagaaagg
    30301 cttcccgaag gaagccttga tgatcacaaa cctaagattg cctgatgcac tacgcttatc
    30361 aggcctacac ggatattgca atatattgaa tttgcaaagt tttgtaggcc ggattaggcg
    30421 ttcatgccgc ttccggcatg aacaacgagc acattgtcag caaatcaccg tttcgcttat
    30481 gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac ctttgatgcg ctcaataacc
    30541 gcttcatcat tgatgctgtc cagcacgtca cacatccagc cagccagttc tttcgcttcg
    30601 gcttctttaa agccacgacg ggtaatcgcc ggagtaccca cgcgaatacc ggaggtcaca
    30661 aacgggctct tcggatcgtt cggtacgctg tttttgttaa cggtgatgtt agcacggccc
    30721 agagcggcgt ccgcttcttt accggtcagg tttttatcaa ccagatcaac caggaacagg
    30781 tggttatcag tgccgccgga aaccactttg tagccgcgtt ccaggaacac ttctaccatc
    30841 gctttagcgt ttttagcgac ctgctgctgg taagttttga actcaggctc catcgcttct
    30901 ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg gaccgccctg accaccaggg
    30961 aaaacggcag agttcagttt tttgtacagc tcttcgctac cacctttcgc caggatcagg
    31021 ccgccgcgcg gacccgccag ggttttgtga gtggtggtag taacaacgtg agcatgagga
    31081 accgggttcg ggtagacgcc agctgcaacc aggcccgcaa cgtgcgccat atcaacgaac
    31141 aggtaagcac cgatgctgtc agcgatttca cgcattttcg cccagtccac cacgccggaa
    31201 tatgcagaga agccaccgat aatcattttc ggtttgtgtt ctttggcttg tttttccaga
    31261 tcggcgtagt cgatatgacc gctagcatcg ataccgtaag gaacgatgtt gtacagttta
    31321 ccggagaagt taaccggaga accgtgagtc aggtgaccgc catgcgccag gttcataccc
    31381 agaacggtat cacctggttc cagcagcgcg gtgtacaccg caaagttagc ctgggagccg
    31441 gagtgcggct ggacgttagc gtagtcagca ccgaacagtt ctttcgcacg atcgatcgcc
    31501 agttgttcaa cgatatcaac atactcgcaa ccgccgtagt agcgtttgcc cggataacct
    31561 tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag
    31621 ttttcggagg cgatcagttc gatgtgctct tcctgacgta ctttttcctg ctccatagcc
    31681 tgccacagtt cggcatcata atcggcaatg ttcatttcac gctttaacat ccgcatctcc
    31741 tgactcagct aacaataaaa tttttggcct ttataggcgg tcctgttgga caacggcgaa
    31801 cagtataacc gaatcattgt gcgataacag gtcttgacaa aggaatttac gcaaacgatt
    31861 accttcaggc tgcgcaaggc tttggagaat aaagagcttg caaccggaaa cggatttctt
    31921 ttcaggtttg tgatgcaaat ttttcacttc atcacattct ttctgaaaaa caccaaagaa
    31981 ccatttacat tgcagggcta ttttttataa gatgcatttg agatacatca attaagatgc
    32041 aaaaaaaagg aagaccatat gcttgacgct caaaccatcg ctacagtaaa agccaccatc
    32101 cctttactgg tggaaacggg gccaaagtta accgcccatt tctacgaccg tatgtttact
    32161 cataacccag aactcaaaga aatttttaac atgagtaacc agcgtaatgg cgatcaacgt
    32221 gaagccctgt ttaacgctat tgccgcctac gccagtaata ttgaaaacct gcctgcgctg
    32281 ctgccagcgg tagaaaaaat cgcgcagaag cacaccagct tccagatcaa accggaacag
    32341 tacaacattg tcggtgaaca cctgttggcg acgctggacg aaatgttcag cccgggccag
    32401 gaagtgctgg acgcgtgggg taaagcctat ggcgtactgg ctaatgtatt tatcaatcgc
    32461 gaggcggaaa tctataacga aaacgccagc aaagccggtg gctggaaagg tactcgcgat
    32521 ttccgcattg tggctaaaac accgcgcagc gcgcttatca ccagcttcga actggagccg
    32581 gtcgacggtg gcgcagtggc agaataccgt ccggggcaat atctcggcgt ctggctgaag
    32641 ccggaaggtt tcccgcatca ggaaattcgt cagtactctt tgactcgcaa accggatggc
    32701 aaaggctatc gtattgcggt gaaacgcgaa gagggtgggc aggtatccaa ctggctgcac
    32761 aatcacgcca atgttggcga tgtcgtgaaa ctggtcgctc cagcaggtga tttctttatg
    32821 gctgtcgcag atgacacacc agtgacgtta atctctgccg gtgtcggtca aacgccaatg
    32881 ctggcaatgc tcgacacgct ggcaaaagct ggccacacag cacaagtgaa ctggttccat
    32941 gcggcagaaa atggcgatgt tcacgccttt gccgatgaag ttaaggaact ggggcaagca
    33001 ctgccgcgct ttaccgcgca cacctggtat cgtcagccga gcgaagccga tcgcgctaaa
    33061 ggtcagtttg atagcgaagg tctgatggat ttgagcaaac tggaaggtgc gttcagcgat
    33121 ccgacaatgc agttctatct ctgcggcccg gttggcttca tgcagtttgc cgcgaaacag
    33181 ttagtggatc tgggcgtgaa gcaggaaaac attcattacg aatgctttgg cccgcataag
    33241 gtgctgtgat ttgatgttgc cggatggaaa catccggcaa cccttgacgc ggtttaaatt
    33301 gccgcgtcgt cttcttcacc ggtacggata cgaataaccc gtgccacgtc gaagacgaag
    33361 attttaccgt caccgatttt gccggtttgc gccgtgcgaa taatggtatc gacacaggta
    33421 tcgacaatgt cgtccggtac gacaatctca attttcacct tcggcagaaa atccaccata
    33481 tactccgcgc cgcggtacag ctcggtatgg cctttctggc ggccaaaacc tttcacctca
    33541 gtgaccgtca tgccggtaat gccgacttcc gccagtgctt cgcgaacatc gtccagcttg
    33601 aagggtttaa taatcgcatc aatctttttc atgctattcc ttgaaaaggt cgcctgtctt
    33661 ttgatctgct aaacgtaaca cataacgcca attcattcct tgaaatcgtt tgcatccagc
    33721 tcgtgtcggg aaagcagttt ataaaattct gtccggttgc gccccgccat tctcgccgcg
    33781 tgggtgacgt tgcctttggt gatttgcagc agcttacgca aatagttgag ttcaaactga
    33841 ttacgtgcct caacaaaggt tggcagcgcc gtgttttcgc cctccagcgc ctgctctact
    33901 aatgcatcgc taatcaccgg agatgaggtc agcgccacgc actgttcaat cacgttgacc
    33961 aactggcgta cattacccgg ccagctagcg gtcatcaggc gtttcattgc atcggtggag
    34021 aacgcgcgga caaacggttt atgtcgctct gccgcctggc gcaacaggtg atttgccagt
    34081 agcggaatgt cttctgtgcg ctctgccaga gccggaattt tcaggctgac aacgttgagg
    34141 cggtaataga gatcttcgcg gaattccccg cgcgccatcg cttttggcag gtcgcggtga
    34201 gtggcggaaa taatccgcac gtcgatatca atatcgcggt tactgcccag cgggcgcact
    34261 ttacgctcct gcaacacgcg cagcagtttg acctgcaacg gtgcgggcat atcgccaatc
    34321 tcatcgagaa atagcgtacc gccttccgcc gcctggaata aaccttcgcg attgctgaca
    34381 gcgccagtaa acgcgccacg cgcatgacca aacagttccg actccagcaa ttgctcgggc
    34441 aatgcaccac agttaatagc aataaacggc ttgctgttac gcgggctggc gttgtggata
    34501 gcctgggcga aaatctcttt cccggtgccg ctctggccgt taatcaaaac gctaacgtct
    34561 gattgcgcca ccagccgcgc ctgttccagc aaacgcagca tcagcgggct gcgggtgaca
    34621 atcgcctcgc gccaacgttc gtcggtggct ggcgcggatt gttccagcgc atcgtcaatt
    34681 gcctgatata gcgcgtcttt atcgacaggc ttggtgagga aactaaaaac gccctgctgt
    34741 gttgcagcaa cggcatcggg aatagaacca tgcgcggtaa gaataattac tggcattccc
    34801 ggctgcactt tctggatttc agcaaacagc tgcataccgt ccatttcatc catccgcagg
    34861 tcgctgatga ctaaatctac tttttcgcga ttcagtaccc gtaatccttc agcaccactt
    34921 tccgccgtga ccacgctgta gccttcgctg gtcaggcgca ggccaagcag tttcagcaat
    34981 cccggatcgt catcgaccaa taataaatgc gcaggtttat ggctcatcag gagtgacctc
    35041 atgggtggat ggcgcgggct tttcaccctc gtgcggcgta tccggcgaga aatttccagc
    35101 cggtttgcgg gtcgagagct ggcgttcaat atcggtcagg ttttccagct tgcgggtagt
    35161 gagttccagt tgctgttgta gaacgtgatg ttgctggcgc aaagtatcca gctcgctgtc
    35221 gctggactgc tggagtttgc tgtagcgttg gcgctcttcc gccagttgca gttgcagcgc
    35281 ctgaccgtcg cgccaaagtt gatacagagg gcgaacctgt gccgggattt cggtacttag
    35341 cgcatcaatg cgcgcgacca gctggcggcg ctcatacggc gtaattttgg catcggcgag
    35401 caaaatccct tgtttaaagg tattttgcca gctgccgtcg ttatattggc gagcttgctg
    35461 acgcgactgc gcaggcatta aacgatcagc acaatccatc gcccgcagcc agtaaagcgg
    35521 attggtttcg gttgatttac cttgcagcgc ccagatgtcg ctacattcag tagaaagata
    35581 gtcagccagt tgataaaccg gaattttttc ttctgctggc gtattaatgg ctggcttatt
    35641 gtgactctgc acgcaaccca gcaatgccag acatggcagc cctgccagcc acagccgtcg
    35701 gggcaataat cgttgaaaaa tgtgtcgcat attcaccaga cttagcgcct ttcccagtgg
    35761 gcgtaattgt tacagacagt ctggacatgg acagcgcgga gaaaccggag cgtacatgtc
    35821 gtacgtgagg atttcgagca ctgcccaggg ccaaactggc aaataaaata gcctgatggg
    35881 ataggctgtt agcattattt cgtgtttttc gacgacggca attcaatgcg gaagcaaacg
    35941 tcttgcccgc tctcgtcgac cagatacagt tccccttgca tacggcgaat acaatccctg
    36001 gcaatgctta atcccagacc gctgcccttc accgcccctt ttcgctggtg gcttccctga
    36061 aaaaagggtt caaagatcat ggcgcgttcc tcttgcggaa tgggtgtgcc tgtattgatg
    36121 acatcaatat aaacccgcgc gccatgtaaa ctgctgcgaa ggcaaatgtt accggattca
    36181 gtcccgtagt gcacagcatt ggagtaaaga ttatccagta cgctcatcag cagcattggc
    36241 tccgccaggc aagctgttgc ttggagatcg acgtcggtat gcatcatttt agcccgtgcg
    36301 ggcaggctat gagcagaaac tactgtctcc accagcggtg ctaattcaac attctccagt
    36361 tccaccgcac tgtccgcctg tttacggttg taatcaagca gttgttcgat cagtttttgc
    36421 aaattgcggc tgctgctatc gaggatgctc accacctctt tttgctctgg cgtaagcggc
    36481 ccgacaacct ggtcagccag taattcagtg ccctcgcgca tacttgccag cggcgttttt
    36541 aattcatgag ataaatgtct taagaattga tgacgttggg attccagcca tgacaggcgc
    36601 tcacttaacc aaagaatacg ttgcccaacc gagcgtaact cgctcggtcc actgaacgag
    36661 acactattgc ccagagaacg cccttccccc agccggttga tcatgcgctc gatatttttc
    36721 accggaccga taatcatcct cgtgaaaagt agcaccatca ccagactcac cagaaataac
    36781 accagcgatt gccaaccaaa atattgccca cgttcggcga tttcacgctg aagttgctgc
    36841 ccacgagaga acaccactgt gcgcgtggcc tgcaccattt cggtattggc actggcaaag
    36901 gcttcaagac gcgcggcggc ggcggcatcg ggaccactgt tgttacactg aagttgggcc
    36961 agattgttca agtcctgacg caatgcctgg taaagtttat cgtctggcag tacgcctgcg
    37021 tgggcatcga gcatttcgct gtaacgcttg cgctggcttt gataaacctt cgcaagcgta
    37081 gggtcgtcca gcacgcaata ctgacggtag ctacgctcca tctccagtgc cgcgttggtc
    37141 atcgcttcac tacgccgggc atcaataagc gtagtgcggt taaccagcgc cgcctgatcg
    37201 ctaagtgcat tcaggctttg ccaggcttgc catgccagca ccaacagggg tagcagaatc
    37261 agcaaaaatg ccagcattac cagttgtcgt aatgagcggg gaaaaacggg ccagcgtttc
    37321 aaggtgttac tctcgtcaga cgcgaatagc ctgatgctaa ccgaggggaa gttcagatac
    37381 aacaaagccg ggaattaccc ggctttgtta tggaataagg cggtgcctaa ctcgacgttt
    37441 cgcccgatgg ttgatatagc tacgctgata tcagaagttg gacggcaggc accttgttgt
    37501 gcgtcattcg aattttatgt agcacgtccc gaaggggctg acataagtcg gtgaatgagc
    37561 cactggttac tattatgcag taactgtgcc aataaagaaa atagtttggt aacgtactga
    37621 ttatacgccg ctttgaggga tttatggctt cttcgctatt tgaatgtaaa tcagccactg
    37681 tgtcgctaaa aagagacaac ttaagataaa cttattagat aatattaaaa tcaataagtt
    37741 aaatgtcgcc agaaagcgac acggcacacc acccattgtc gtgatttaca gacacaaaaa
    37801 agctcccgaa gttgggagct tataatagcg gttggtgctt aatgccggat gcggcgtgaa
    37861 tgcctcatcc gggctgcaaa accaatgggc tgacgactta ccccaactgc ttacgcgcat
    37921 tgcggaaaat gcgcatccat gggccatcct cgccccagtt ttccggatgc caggagttgc
    37981 tgacagtacg gaaaacacgt tccgggtgcg gcatcataat ggtgactcga ccactttcag
    38041 tcgtgactgc cgtaatacca ttcggagaac cgttcgggtt agccgggtag gtttcagtga
    38101 ctttgccgaa gttatcgaca tagcgcagtg ccaccagccc tttgctttcc agtgccgcca
    38161 gatgcgccgc atcacgcact tccacgcgcc cttcaccatg agatacagca atcggcattt
    38221 gcgagcccac catcccctgc aacagcagag acgggctttg ggttacttca accaggctga
    38281 aacgcgcttc aaagcgatcg gaggtattgc gcacaaaacg tggccacaac tcactacctg
    38341 ggatcagttc gcgcagatta gacatcatct ggcaaccgtt acataccccc agcgccagcg
    38401 tttgcggacg gtggaagaag gttgcaaact catcgcgtac acggtcattg aacaggatag
    38461 acttcgccca accttcaccg gcacccagca catcaccgta ggagaaacca ccgcacgcga
    38521 ccagggcgtg gaaatcctcc aggcccgtgc gtccagccag caggtcactc atatgcacat
    38581 cgattgcatc gaagcctgca cggtggaaag cagccgccat ttcaacatgc gagttcacgc
    38641 cctgctcacg cagtacggca actttcggac gtgcgccagt agcaatatac ggtgctgcca
    38701 catcttcgtt gatatcgaac gacagtttca cattcaggcc cggatcggcg tcgttggatt
    38761 tcgcctgatg ttcctgatcg gcacattcag ggttgtcacg caggcgctgc atctgccagg
    38821 tagtttctgc ccaccagaca cgcaacgtgg tgcggctttc gctgaataca gtctgcccgt
    38881 tggcggtaat cacaaaacgg tcaccggaaa ccgcctggcc aacataatgg acgcaatcag
    38941 caaggccatg ctgtgccagt accgcttcaa ctgctttacg gtcagcggct cgaacctgaa
    39001 tcaccgcacc cagttcttcg ttaaacaacg ccgccaggcg atcgtcaccc agagtggcga
    39061 tatccgcgtc aatgccgcaa tgaccagcaa aggccatttc cgccagcgtt accagcaggc
    39121 cgccatcaga gcggtcgtgg tacgcgagca gcttacgctg tgctaccagc gcctgaaccg
    39181 cgtcatagaa acctttcagt tgcgcgacgt cgcgtacatc tgccggtttg tcgccaagct
    39241 gacgataaac ctgcgccagc gccgttgcgc ccagcgcgtt attgcctttg cccaaatcaa
    39301 tcagcagcag tgcgttatct tcggtagaaa gctgtggcgt gatggtgtga cgtacatctt
    39361 ccacgcgggc aaatgcagaa ataaccagcg acagtggcga cgtcatttcg cgctcttcgt
    39421 taccttcctg ccagcgggtt ttcatcgaca tagagtcttt acccaccggg atcgtcaggc
    39481 ccagagccgg acaaagttct tcgcccacag ctttaacggc ttcatacagg cccgcatctt
    39541 cgcctggatg acctgccgcc gccatccagt tggcggaaag tttaatgcgt ttgatatcgc
    39601 caatctgtgt tgcggcaata ttggttaacg cttcaccgac cgccagacgg gcagaggcgg
    39661 cgaaatccag cagcgcaacc ggcgcacgct cgccaatcgc catcgcttcg ccgtagtagc
    39721 tatcgaggct ggcggtagtg accgcgcagt tagcgaccgg cacctgccac ggacccacca
    39781 tctgatcgcg cgctaccatg ccggttacgc tgcggtcacc aatggtcacc aggaaagttt
    39841 tctccgccac ggtcggcagg tgcagcacgc gtttcaccgc gtcagcaatg gtaatccctt
    39901 cacgaaccag cgcatcaccc ttcgctttca gcgtttgtac ttcgcgggtc atcttcggcg
    39961 ttttaccaag caggacgtcc agcggcagat cgatcggctg attatcaaaa tgacgatcgt
    40021 gcaaagaaag atgcagttct tcggtcgctt caccaatcac cgcgtatggc gcgcgctcac
    40081 gcttacacag ttcgtcaaac agcggtaact gatcggcagc aaccgccagc acgtagcgtt
    40141 cctgggattc gttacaccag atttccagcg ggctcatgcc cggttcgtcg ctcaggatat
    40201 cgcgcagttc gaatttaccg ccgcgcccgc cgtcgctcac cagttccggc atggcgttag
    40261 aaagaccgcc tgcgccaacg tcgtggataa acaggattgg gttggcatca ccaagctgcc
    40321 agcaacggtc gatcacttcc tggcagcgac gctccatctc cgggttgtcg cgctgtacgg
    40381 aagcgaagtc gaggtcggca tcagactgac cagacgccat ggaagacgcc gcgccgccgc
    40441 caagaccgat gttcattgcc gggccgccga gaacgaccag cttcgcacca acgttgatct
    40501 cgcctttttg tacgtgatcg gcacgaatgt tgccgatccc gcccgccagc atgatcggtt
    40561 tgtgataacc gcgcagctct tcgccattgt ggctgttcac tttttcttca taagtacgga
    40621 agtagccgtt cagtgccgga cgaccaaatt cgttgttaaa tgccgcgccg cccagcgggc
    40681 cttcggtcat gatgtccaac gcggtgacaa tgcgctcagg cttaccgaaa tcttcttccc
    40741 acggctgttc gaagcctgga attcgcaggt tggaaacgga gaaacctacc agaccagctt
    40801 tcggctttgc gccacgcccg gtggcacctt catcgcggat ttcaccgccg gaaccggtcg
    40861 ccgcgcccgg ccacggagaa atcgccgtcg ggtggttgtg agtttcgact ttcatcagaa
    40921 tatgcgccgg ttcctgatgg aaatcgtagc ggcccgtttc gtggtcagca aagtagcggc
    40981 ccacttcaga cccttccatg acggcggcgt tatctttata agcagagaga acgtgatccg
    41041 gcgtagtttc gaaggtattt ttgatcatct tgaacagcga tttcggctgc tgttcaccgt
    41101 cgataatcca gtcggcgtta aaaattttgt ggcggcagtg ctcagagttc gcctgagcga
    41161 acatatacag ctcgatgtcg ttcgggttac gaccaagctt tgtgaaagca tcctgcagat
    41221 agtcgatttc atcttccgcc agtgccaggc caagacgcag gttagcgtcg attagcgcct
    41281 gacggccctg tcccagcaaa tcaacgctgg tgaccggagt cggttgatgg tgtgcaaaca
    41341 gctgctctgc atcatctaaa gcaaaaaaga ccgtttccat catgcggtcg tgcagttcag
    41401 cggtaacctg ctgccattgt tcattggtca gcgtaccggc ttctatatag taagccacgc
    41461 cgcgctcaag acggtttacc tgttgcagcc cgcagttatg ggcaatatcg gtcgctttcg
    41521 aagaccaggg agagatggtg ccaggacgcg gggtcaccag caggagtttg ccttgcgggg
    41581 catggctggc gagtgccggg ccatatttca gcaggcgttc aagttgtgcg tgctcatcat
    41641 cgtttaacgg cgcattgagg tcagcaaaat ggacatactc ggcgtaaata gtgtgaaccg
    41701 ggagcctggc agcctgaaaa cgtgccagca gtttgttgat tcggaatgcc gacaatgcag
    41761 gcgaaccacg cagaatttcc atcataagtc tctcgtcttc taagctttcg gtgtacccaa
    41821 gggggggaaa cgggcgtcat tataaagaat ctgatgcgct gacgaaaccg tttgcgtgga
    41881 aataaaatca ccatcgcgaa ttagcaacgc gtgccgccaa tggctgtaat aagttgccaa
    41941 ctggcgcagg tttacgcaaa atgccgctca tttatgagta aacctttcac tattattacg
    42001 ttttttcaag ctgggacgcg cacgacacag agaattaact aattgaaaaa attaaagatt
    42061 aattatctgt tcatcggcat tctggcactg ctactcgcgg tcgctctctg gccatccatt
    42121 ccctggtttg gtaaagccga caaccgtatc gccgccattc aagcgcgggg agagttgcgt
    42181 gtgagcacca ttcatactcc cctgacttat aacgaaatca acgggaaacc ttttggcctg
    42241 gattacgaac tggcgaaaca gtttgccgat tacctcggcg taaaactgaa agtgaccgtg
    42301 cggcagaata tcagccagct gtttgacgac ctcgataatg gtaacgccga cctgctggcg
    42361 gcaggactgg tctataacag cgaaagggtt aaaaattatc agccaggccc gacctactac
    42421 tccgtttcac aacaactggt ttataaagtg ggtcagtatc gcccacgtac gctgggcaac
    42481 ctgacggcgg agcaactcac cgttgcaccg ggtcatgtgg tggttaacga tctccagacc
    42541 ctgaaagaaa caaaattccc ggaattaagc tggaaggttg acgacaaaaa aggctctgcg
    42601 gaattaatgg aagatgtcat cgaaggaaaa ctcgattaca ccattgctga ttctgttgcc
    42661 attagcctgt ttcagcgcgt tcatccggaa ctcgccgtag cgctcgacat taccgatgaa
    42721 caaccggtga cctggtttag cccgttagat ggcgataata ccctttccgc cgctctgctc
    42781 gatttcttca acgaaatgaa tgaagacggt acgctggcac gcattgaaga gaaatacctg
    42841 gggcatggcg atgattttga ttacgtcgat acgcgcacat ttttacgcgc cgtcgatgcg
    42901 gtactgccgc agttaaagcc cctgtttgag aaatacgccg aagaaattga ctggcgtttg
    42961 ctggccgcta ttgcttatca ggaatcgcac tgggatgcgc aggccacttc accgacgggg
    43021 gtgcgcggca tgatgatgtt aaccaaaaat accgcgcaaa gcctcggcat tacggatcgt
    43081 accgatgccg aacagagcat cagcggcggc gtgcgttatt tgcaggatat gatgagtaaa
    43141 gtgccggaaa gtgtgcctga gaacgaacgg atctggtttg ccctcgccgc gtacaatatg
    43201 ggctatgcgc atatgctgga tgcccgcgcc ctgacggcaa aaactaaagg taatcctgac
    43261 agttgggctg acgtaaagca gcgtctgcct ttacttagcc agaaacccta ttacagcaag
    43321 ctgacttacg gctacgcgcg tgggcatgaa gcctacgctt atgtcgaaaa tattcgtaag
    43381 tatcagatta gcctggtggg ttatctgcaa gagaaagaga agcaggctac agaagcggcg
    43441 atgcaactgg cgcaggatta tccggcggta tcgcctacgg agttgggcaa agagaaattt
    43501 ccttttctct cgtttctttc ccagtcgtca tcaaactatt tgacccactc tccctctctg
    43561 ctgttttcca ggaaagggag tgaagagaaa caaaattaat ccgtcgagga tcgcgctttt
    43621 ttctgcgctt taatttcctg gcggcgcatg cgaaagaagt cactgagcaa cgccgcgcac
    43681 tcatccgcca gtattccttc cgtaatttcc actcggtgat tcatacccgg atgatgcagc
    43741 acatccatta aagatcccgc agcgccagtt ttcgcgtcac gcgcaccaaa gaccacgcga
    43801 ccaatgcgac tgtggatcat tgctccggca cacattacac agggttcaag cgtgacatac
    43861 aacgtggcgt cgatcagacg ataattttgc atcaccagac cgccctgccg cagagccatg
    43921 atttctgcat gtgcggtggg atcatgtcga ccaatcggac ggttccaacc ttcgccgatt
    43981 acccgattgt tatgcactaa taccgcgccg accggcactt cccgctcatc ccaggcacgc
    44041 ttcgccagcg tcattgcatg acgcatccag tattcgtggc taaattcgac ttcagacaaa
    44101 aagaaaactc cggttataaa agcgcggcgc attatacacg gacgctatgc tttactctat
    44161 tccagttgct ggagttcacc gcgcggggtt acccgccaac gatgctggca gaaataaagc
    44221 agcgggttgt cctgattact gtcgctatag ccactgtaca gccgcagcgg cgtgccgatt
    44281 ttgcgctcca gttgtgcgac cttttcatgt cccagacaac gcatcgtcaa tacccaacca
    44341 ccatagccac gctgaatttg gctggcgata agattaaccc gcggcagcca gggcgtatcg
    44401 aaataaaccg cttcaaccag cggctgcgga gagccggtaa tcaaccagat atcagcatcg
    44461 gaacttaaca ggtaggtggt taatcgctcc tgaaccagcg gaaaggcggt aacattgtca
    44521 cgaaaccaac gtacgaaatc ggcctgcaac gcctgcaaac gcgcttcatt gtgaccaaaa
    44581 gtgcatcccc acagaagcag actcatcggc cagcgtgccg cacgaccttt tatcaataac
    44641 gcaatggcta tgatcggcaa caacggtagg acaagcaacg cattcagcgg ttggcgacgc
    44701 agtaaataac gcaaaaaact gccgaacata tcctgctgat gcaatgttcc atctaagtca
    44761 aaaaacacca cacgacgctc gtgagttgcc aaaccatact cctctggact gaaacatcct
    44821 gattaatttt cgctactaca tagcctaaca gatagatcat cacttttccg gcaacaatcg
    44881 accgcatcat ggctaactgg aatttttaat tcatggcaat tagcggcaat ggaatataaa
    44941 attcactcgc gtttgtctca tattgcctgt tgtcgccact atttcatcgc gaggaaagat
    45001 atgaacggct tacttcgtat ccgtcagcgt taccaggggc ttgcccagag tgataaaaaa
    45061 ctggcggatt atctgctgct acaacctgat acggcgcgcc atttaagctc tcagcaactg
    45121 gccaacgaag ccggagtcag tcagtccagc gtcgtgaagt tcgcgcaaaa actcggctat
    45181 aaaggttttc cggcgcttaa gttggcgttg agtgaagcgc tggcaagcca gccggaatcg
    45241 ccctccgtgc ccattcataa ccaaatccgc ggtgatgatc cgttacggct ggtcggcgaa
    45301 aaactgatta aagaaaatac tgccgctatg tacgcaacgc taaacgttaa tagtgaagag
    45361 aaactgcatg aatgcgtaac aatgttgcgc tctgcgcggc ggataattct gaccggtatt
    45421 ggcgcttcgg gtctggtggc gcaaaacttt gcctggaagc tgatgaagat tggcttcaat
    45481 gctgccgcag tgcgcgatat gcatgcgcta ctcgctaccg tacaggcgtc gtcccctgac
    45541 gatctgttat tagccatttc ttacaccggt gtacgacgcg agttaaacct ggcggcagat
    45601 gagatgctgc gagtgggcgg aaaagtgctg gcgattaccg gctttactcc caatgccctg
    45661 caacagcgcg cttctcattg cctgtatacc attgccgaag aacaagcgac aaacagtgct
    45721 tcaatctctg cttgccacgc gcagggaatg ttaacggatt tgctgttcat tgcgctgatt
    45781 cagcaggatc tggaactggc accagaacgt attcgtcata gtgaagcgct ggtgaaaaaa
    45841 ctggtctgag taaagaatgc gcgtataatg cccgcccggt ttgtgttgtt ttgagagttt
    45901 ccttatggcg ctgttaatca ctaaaaaatg catcaattgt gatatgtgtg aacccgaatg
    45961 cccgaatgag gcgatttcaa tgggcgatca tatctatgag attaacagcg ataagtgtac
    46021 cgaatgcgta gggcactacg agacaccaac ctgccagaag gtgtgcccga tccccaatac
    46081 tattgtgaag gatccgcaac atgtcgagac ggaagaacag ttgtgggata aatttgtgct
    46141 gatgcatcac gcggataaaa tttaatattc tgctcctgga gtagactatt gttgacttta
    46201 tag
//
LOCUS       DAYPHN010000016        45919 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000016 DAYPHN010000000
VERSION     DAYPHN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.016"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(52..333)
                     /gene="yehE"
                     /locus_tag="ACG0BW_000938"
     CDS             complement(52..333)
                     /gene="yehE"
                     /locus_tag="ACG0BW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFK7257933.1"
                     /translation="MNKYWLSGIIFLAYGMASPAFSSQTATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYDVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(596..1705)
                     /gene="apbC"
                     /locus_tag="ACG0BW_000939"
     CDS             complement(596..1705)
                     /gene="apbC"
                     /locus_tag="ACG0BW_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFK7257934.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1837..3870
                     /gene="metG"
                     /locus_tag="ACG0BW_000940"
     CDS             1837..3870
                     /gene="metG"
                     /locus_tag="ACG0BW_000940"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFK7257935.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWHGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAATPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4011..7808
                     /locus_tag="ACG0BW_000941"
     CDS             4011..7808
                     /locus_tag="ACG0BW_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFK7257936.1"
                     /translation="MRHFIFQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAAKAELKLIAEKTKKGYVENASANVHIPPITKATPEVETSLESKNKRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLYKDGQIWKRIANNTRAYDPDNNYRSYPENWQQAF
                     AELQIRVKGNQPTGSAQSDAALLWGFWNSYSQDELVDDLVIRCGLESAVEIALLALQL
                     KYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTTAEKYDFPPLYHD
                     FRAYLAMLLANNGVRGVSRILLEFTEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAALAAYATLLAIHEDKEWRKALVKLITATPEL
                     VTDVIPRVNAKAAGILSECRTQLVAEECEYATADMLPELFVAPPWVINKKTNVIPVFD
                     LPVLPIPAVTDITPGITELISHTDISRFSEIAQFQSSQQTLFTDLPLIEKESWETSFI
                     PFTPEQQILWQLGFNEWLHCEDDLHEKKYIPQSAVDALLRFDFPALKAEFAKYHNNAN
                     KSWNLSALCYLPGQQAISFLNQIIIEERYSGEKEILAVFGSTAIPAFMTCLQRDHQRL
                     WIFTLFIGASELALPMAQRLQKKMAYKDAVNWLANNPRHATAGLLPLALGKPCQNREY
                     ARQALRLLVKLNQRETIEEIARRYNQPDVLAALATLFDSDPLEEYPAKIAPPPGFYQF
                     TLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITAYAGLATIKETFTRESLADFGWDL
                     YTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVSGLDVLADI
                     GSDVALMLLNGIAKKIKFVALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDSSGS
                     LTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKQNQSDDKTLATDAVNLFKQLKKD
                     VRAIASQQVDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIAC
                     FRVAEDSTYSDAQDELFTLPAGNIGIPHVLEIPPESAAAFRQIYVDYELLPPFQQLER
                     GSYHLADNERNVHELSRWDGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGALE
                     LETEHFSLIYGETGYSDLLPVESVKITASYDRYGKQSSPTFSVLDNITASELINDIES
                     LFD"
     gene            7821..11582
                     /locus_tag="ACG0BW_000942"
     CDS             7821..11582
                     /locus_tag="ACG0BW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFK7257937.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHISWGKVSTNGQSQVKSFAD
                     AAAAEKAELKLIAEKTKKGYVEEVSVITPTSVPVQVIECPEIAPLPQDKPVFVGDNLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDPVPQENELTLLRSVAANTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTTLPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQFIRFDWDYDAHLITFTPDDRQPGYLLRFASVEMRLRKHFSLANDDVW
                     QRCADKLIAALGNMPAWHQPLVALLLPEKPEVAHEIARHFCGQKGLYALEWLKLTVTD
                     AQVLADLEKYYPGQPGQVFDDYYGGNIWCATALQEQGATALARFAHYATGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKAFVRFPHAALAALAELLAQKDEKRWRMMLMT
                     MLISQPILAEQVIPWLSTPAVAVLKSCQQQLKQPSNHASADMLPAILVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARSWLLKYPEHAITGLLPAALGKAGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDALDNHPTKIPTLPAFYQPSLWTRPVLKANAQSLPDSALLR
                     LGEMLRFPQEEALYPGLLQVKAACTADSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAISSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFM
                     RDASGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSTENELLACFRVAEDNSYSTADDDLFTLPEG
                     DISVGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTETERNASELIRWAG
                     RKCPSGRVMGLANKGWIKGTPLDAGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSHSTQEAQFSVLDDITASELINDIEALFE"
     gene            11592..15224
                     /gene="yehI"
                     /locus_tag="ACG0BW_000943"
     CDS             11592..15224
                     /gene="yehI"
                     /locus_tag="ACG0BW_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFK7257938.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRSWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLLVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPWTHFHSKEWLKVVANDPRAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRILLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMALQPQLAEE
                     VMPWLSTQARAVLNSFLSAPSKTVIDSTDNSQMPEILVSPPWRSKKKMTVPRLDLAPF
                     ELTPQVYWQPGERERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAACNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRNGHVEVLINIWEND
                     SYSRYRREKSIWNLYLLAQLPREIALTFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQSPIEIYPT
                     RIPKAPDFWQPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYCGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTLILAWPQEGKSAR
                     AVSGLNILTQMNNDMALIQLHHISQRAKSRPLRDNAAEFLHVVAENRGLSQEQLADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLHVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15285..15590
                     /locus_tag="ACG0BW_000944"
     CDS             15285..15590
                     /locus_tag="ACG0BW_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7257939.1"
                     /translation="MSVQKELVAIYNYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FQLHPTFNDADPLINDALIYIRDEFIDERKLRGESPETVIAIFNRELQNIFNQEIEK"
     gene            16774..17862
                     /gene="yehL"
                     /locus_tag="ACG0BW_000945"
     CDS             16774..17862
                     /gene="yehL"
                     /locus_tag="ACG0BW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFK7257940.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLMAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17873..20152
                     /gene="yehM"
                     /locus_tag="ACG0BW_000946"
     CDS             17873..20152
                     /gene="yehM"
                     /locus_tag="ACG0BW_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFK7257941.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELLLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEEIQTLLLRATRMDNSDTLWDHLFEDESQQTILPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWREYTQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQHGSTLTLAGNGEEQWKLTQPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPEEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERCSQAVHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALGSLQHWGVFHV"
     gene            20145..21281
                     /gene="yehP"
                     /locus_tag="ACG0BW_000947"
     CDS             20145..21281
                     /gene="yehP"
                     /locus_tag="ACG0BW_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFK7257942.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDGNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSMLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMALTLVNVGAQIAAMTPGELATWLAENLQS"
     gene            21278..23278
                     /gene="yehQ"
                     /locus_tag="ACG0BW_000948"
     CDS             21278..23278
                     /gene="yehQ"
                     /locus_tag="ACG0BW_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFK7257943.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTPSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQTAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENAPAAKRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLAQFRNTESEARVEAMNNGVLICEQLFPMLQQQG"
     gene            23403..23864
                     /gene="yehR"
                     /locus_tag="ACG0BW_000949"
     CDS             23403..23864
                     /gene="yehR"
                     /locus_tag="ACG0BW_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFK7257944.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23906..24376)
                     /gene="yehS"
                     /locus_tag="ACG0BW_000950"
     CDS             complement(23906..24376)
                     /gene="yehS"
                     /locus_tag="ACG0BW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFK7257945.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(24423..25142)
                     /gene="btsR"
                     /locus_tag="ACG0BW_000951"
                     /gene_synonym="yehT"
     CDS             complement(24423..25142)
                     /gene="btsR"
                     /locus_tag="ACG0BW_000951"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFK7257946.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(25139..26824)
                     /gene="btsS"
                     /locus_tag="ACG0BW_000952"
     CDS             complement(25139..26824)
                     /gene="btsS"
                     /locus_tag="ACG0BW_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFK7257947.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            27046..27777
                     /gene="mlrA"
                     /locus_tag="ACG0BW_000953"
     CDS             27046..27777
                     /gene="mlrA"
                     /locus_tag="ACG0BW_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFK7257948.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27837..27944
                     /gene="yohO"
                     /locus_tag="ACG0BW_000954"
     CDS             27837..27944
                     /gene="yohO"
                     /locus_tag="ACG0BW_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFK7257949.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27925..28656)
                     /gene="yehW"
                     /locus_tag="ACG0BW_000955"
     CDS             complement(27925..28656)
                     /gene="yehW"
                     /locus_tag="ACG0BW_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFK7257950.1"
                     /translation="MKMLRDPLFWLIALFVALLFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDVSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(28661..29587)
                     /gene="yehX"
                     /locus_tag="ACG0BW_000956"
     CDS             complement(28661..29587)
                     /gene="yehX"
                     /locus_tag="ACG0BW_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFK7257951.1"
                     /translation="MIEFSHVSKLFGIQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDNGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(29580..30737)
                     /gene="yehY"
                     /locus_tag="ACG0BW_000957"
     CDS             complement(29580..30737)
                     /gene="yehY"
                     /locus_tag="ACG0BW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFK7257952.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWMTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGALNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(30744..31661)
                     /gene="osmF"
                     /locus_tag="ACG0BW_000958"
     CDS             complement(30744..31661)
                     /gene="osmF"
                     /locus_tag="ACG0BW_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFK7257953.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31810..34107)
                     /gene="bglX"
                     /locus_tag="ACG0BW_000959"
     CDS             complement(31810..34107)
                     /gene="bglX"
                     /locus_tag="ACG0BW_000959"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFK7257954.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDATRHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            34303..36018
                     /gene="dld"
                     /locus_tag="ACG0BW_000960"
     CDS             34303..36018
                     /gene="dld"
                     /locus_tag="ACG0BW_000960"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFK7257955.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGS
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(36056..36988)
                     /gene="pbpG"
                     /locus_tag="ACG0BW_000961"
     CDS             complement(36056..36988)
                     /gene="pbpG"
                     /locus_tag="ACG0BW_000961"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFK7257956.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(37162..37749)
                     /gene="yohC"
                     /locus_tag="ACG0BW_000962"
     CDS             complement(37162..37749)
                     /gene="yohC"
                     /locus_tag="ACG0BW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFK7257957.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37919..38497
                     /gene="yohD"
                     /locus_tag="ACG0BW_000963"
     CDS             37919..38497
                     /gene="yohD"
                     /locus_tag="ACG0BW_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFK7257958.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38616..39377)
                     /gene="yohF"
                     /locus_tag="ACG0BW_000964"
     CDS             complement(38616..39377)
                     /gene="yohF"
                     /locus_tag="ACG0BW_000964"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFK7257959.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPWAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(39430..40866)
                     /gene="mdtQ"
                     /locus_tag="ACG0BW_000965"
     CDS             complement(39430..40866)
                     /gene="mdtQ"
                     /locus_tag="ACG0BW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFK7257960.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPASQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            41090..41173
                     /gene="yohP"
                     /locus_tag="ACG0BW_000966"
     CDS             41090..41173
                     /gene="yohP"
                     /locus_tag="ACG0BW_000966"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFK7257961.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(41546..42493)
                     /gene="dusC"
                     /locus_tag="ACG0BW_000967"
     CDS             complement(41546..42493)
                     /gene="dusC"
                     /locus_tag="ACG0BW_000967"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFK7257962.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPIK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREVVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            42732..43130
                     /gene="yohJ"
                     /locus_tag="ACG0BW_000968"
     CDS             42732..43130
                     /gene="yohJ"
                     /locus_tag="ACG0BW_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFK7257963.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            43127..43822
                     /gene="yohK"
                     /locus_tag="ACG0BW_000969"
     CDS             43127..43822
                     /gene="yohK"
                     /locus_tag="ACG0BW_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFK7257964.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43952..44836
                     /gene="cdd"
                     /locus_tag="ACG0BW_000970"
     CDS             43952..44836
                     /gene="cdd"
                     /locus_tag="ACG0BW_000970"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFK7257965.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44986..45705
                     /gene="sanA"
                     /locus_tag="ACG0BW_000971"
     CDS             44986..45705
                     /gene="sanA"
                     /locus_tag="ACG0BW_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFK7257966.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45708..>45919
                     /locus_tag="ACG0BW_000972"
     CDS             45708..>45919
                     /locus_tag="ACG0BW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFK7257967.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIG"
BASE COUNT        11293 a        12216 c        11521 g        10889 t
ORIGIN      
        1 acacgaatga tttcagcttt ctaaatccga ttaaaaaacc agggtggcaa attaatcgta
       61 gcgatttaac acgattcgac gtttgctatc gctacctgca acaaccactt ctgtcgttac
      121 acctttgact ggcgaagaaa cctgatgacg ggtatcaaca tcatacgtta actgaccaca
      181 atgctgttga ggttgaccat taaccatagc catactgcat gttgggggac tgatcctgcc
      241 attaatcgtt aatgtggcag tctgtgaaga gaacgcggga gaagccattc cataggcaag
      301 aaaaataatt cctgacagcc agtacttatt cattgaacgt tatccctgta gtaaaggtta
      361 tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca cagccacgaa
      421 ggatgataat gaagcattgc ctgtacgatc aatcgacttt atagagtttc ggacgaaggt
      481 ccgcagaata ttcgcagcat taaataagtg ttcattaatg cggcaaaaac aaatacaccg
      541 catcaatgta acatctctgc ggtgtatgaa aagcaaccaa aaaggttaat acgcgttaga
      601 ccgcgcggaa ggaaatctcg ccaggaatga cttctccctg ccaataaagc tgggctgcaa
      661 cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctccggacgg ctgataaccg
      721 ttggcgtgcc tttatcgaga tcttcacgta aggagatgtg taacggcatc tgccccagca
      781 actgcgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa atcggttcat
      841 ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg agcaccggaa
      901 cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgca atgtcctgcg
      961 gggtggtgac cactaccgcg ccagttactg gaatgttttg tgccagtgtc aattgaatgt
     1021 caccggtgcc tggcggcata tcaagcacca gatagtcgag atccggccac aaggtttcct
     1081 gcaacatctg cattaacgcc ttgctcgcca tcggaccacg ccacaccatc gcattgtcgt
     1141 cagtgaccag ataaccaatt gaattggttg ccagtccatg agacataatc ggtgccatat
     1201 gggtaccgtc cggcgaagtt ggacgctgat tttctgcgcc cagcatggtg ggtattgatg
     1261 ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc agtgccagcg
     1321 ccaggtttac cgccgtggag gatttaccca cgccgccttt accggagctg accgcaatga
     1381 tgttcttcac gccgttaatt cccggctgat tcttcacgcg ttttaaggtg gcgatattgt
     1441 gcgacagttt ccagtcgata gctttcgccc cggtgatgcg cagcagttcg gcgctacatt
     1501 gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt tccacatgca
     1561 acgtgtcgtc catccaggcg acatggtgca acgctttcag cgtggtgagg ttatgcttca
     1621 gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg
     1681 atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa accatcagct
     1741 gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat ttggtactat
     1801 ctaacccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc gaagaaaatt
     1861 ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca tatgctggag
     1921 cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga ggtcaacttc
     1981 atctgcgccg acgatgccca cggcacaccg atcatgctga aagctcagca gcttggtatc
     2041 actccggagc agatgattgg cgaaatgagt caggagcatc agactgattt cgcaggcttt
     2101 aacatcagct acgacaacta tcactcgacg cacagcgaag agaaccgcca gttgtcagaa
     2161 cttatttatt ctcgcctgaa agagaacggt tttattaaaa accgcaccat ctctcagctt
     2221 tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggcac ctgcccgaaa
     2281 tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac ctacagcccg
     2341 actgaactga tcgagccgaa atcggtggtt tctggcgcta cgccggtaat gcgtgattcc
     2401 gaacacttct tctttgatct gccctctttc agcgaaatgt tgcaggcatg gacccgcagc
     2461 ggtgcgttgc aggagcaggt ggcaaacaaa atgcaggagt ggtttgaatc cggcctgcaa
     2521 cagtgggata tctcccgcga tgcgccgtac ttcggttttg aaattccgaa cgcaccgggc
     2581 aaatatttct acgtctggct ggacgcgccg attggctaca tgggttcctt caagaatctg
     2641 tgcgacaaac gcggcgacac caccagcttc gatgaatact ggaagaaaga ttccaccgcc
     2701 gagctgtacc acttcatcgg taaagatatc gtttacttcc acagcctgtt ctggcctgcc
     2761 atgctggaag gcagcaactt ccgcaagccg actaacctgt ttgttcatgg ctatgtgacg
     2821 gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag cacctggctg
     2881 aatcattttg acgccgacag cctgcgttac tactacactg cgaaactttc ttcgcgcatt
     2941 gatgatatcg atctcaacct ggaagatttc gttcagcgtg tgaatgccga tatcgtgaat
     3001 aaagtggtta acctggcctc ccgtaacgcg ggctttatca acaagcgttt tgacggtgtg
     3061 ctggcaagcg aactggctga cccgcaactg tacaaaacct tcacggatgc cgctgaagtg
     3121 attggtgaag cgtgggaaag ccgcgaattt ggtaaagcga tccgcgaaat catggcgctg
     3181 gcagatctgg ctaaccgtta tgtcgatgaa caggctccgt gggtggtggc gaaacaggaa
     3241 ggacgcgatg ccgacctgca ggcaatttgc tcgatgggca taaacctgtt ccgcgtgctg
     3301 atgacttacc tgaagccggt actgccgaaa ctgaccgagc gtgcagaagc attcctcaat
     3361 acagaactga cctggcatgg tatccagcaa ccgctgctgg gccacaaagt gaatccgttc
     3421 aaggcgctgt ataaccgtat tgacatgaag caggttgaag cactggtgga agcgtctaaa
     3481 gaagaagtga aagcagctgc cacgccagta actggcccac tggcagatga tccgattcag
     3541 gaaaccatca cctttgacga cttcgccaaa gttgacctgc gcgtggcgtt gatcgaaaac
     3601 gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cgctggatct cggcggtgaa
     3661 aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaggc actgattggt
     3721 cgtcatacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg tatctccgaa
     3781 ggcatggtga tggctgccgg tcctggcggg aaagatattt tcctgctaag cccggatgct
     3841 ggtgctaaac cgggtcatca ggtgaaataa tcccccttca aggcgctgca tcgacagcgc
     3901 cttttcttta taaattccta aagttgtttt cttgcgattt tgtctctctc taacccgcat
     3961 aaatactggt agcatcagca ttcaactgga taaaattaca gggatgcaga atgagacact
     4021 ttatctttca ggacgaaaaa tcacataaat tctgggcggt ggagcaacag ggaaacgagc
     4081 tgcatattaa ctggggtaaa gtcggcacta acgggcaaag tcaggtaaaa agctttgcag
     4141 atgctgcggc ggcggcaaaa gcggagctta agctgatcgc agagaaaacg aaaaaaggct
     4201 atgtggaaaa tgcttcagca aacgtgcata tcccccccat caccaaagcc actcctgaag
     4261 ttgaaacctc ccttgagagt aaaaacaaac gcccatggct ggctgatgat gcagtcattc
     4321 cggtcactga cgatattaat cgatttgctt ttcctcatcg ctctcgcccc agagagatca
     4381 attatctgta caaagatggc caaatatgga agcgcatcgc gaataacacc agggcatatg
     4441 atcccgacaa caattaccgc tcttatccag aaaactggca gcaggctttt gctgagttac
     4501 aaattcgagt taagggtaat caaccgacag ggagtgcgca atctgatgca gcattgctct
     4561 ggggcttttg gaactcttac tctcaagatg aactggtcga tgacttagtc attcgctgtg
     4621 gtctggaaag tgccgttgaa atcgcccttc tcgctcttca actcaaatat aaaccagtaa
     4681 aaggcgcagt aaccaccatc attccaccta atcacgaagc agaatcgcta cctagttggc
     4741 atcaacgtct atgtcatcat ctttcactcg cttcagaaga tgagtggcaa cgctgtgtag
     4801 ataaagtact cgctgctatt ccctcgctat ccccagcacg acaacctttt gcggcgttac
     4861 tgctccctga acgcccggat attgccaatg ctatggcgct acgttatgca gatcaaaacg
     4921 ttccggcgat gacctggtta agactggtgg taaccgatga cgccgctctg actacagcag
     4981 aaaaatacga cttcccaccg ttgtatcatg attttcgcgc ttatctggca atgttgctgg
     5041 caaataatgg ggtgcgcggt gtaagccgga tacttcttga gtttacggaa gagcattctg
     5101 acaatcccac ttatcttttc gagcgtatca gcgagactga agatcttgtc aaatggctat
     5161 ggaaaacgaa tcacccggat gcgattcaaa ttctgatcct cggtgtgatt ggcaagaaaa
     5221 agcacctgga atacttaagc aaagcctgtc aaaaacatcc cgctgcggct cttgccgctt
     5281 atgctacttt gctggcaata catgaagaca aagagtggcg taaagcgctt gtcaaactga
     5341 ttaccgccac gccagagtta gtcactgacg tcattcccag ggttaatgct aaagcggcag
     5401 gaattttatc tgaatgtcgt actcagttag tcgctgaaga atgtgaatac gccactgcgg
     5461 atatgttgcc ggagctgttt gtcgctccac cgtgggtaat aaataagaaa acgaatgtaa
     5521 tacccgtatt tgatttaccg gtgcttccca tccctgccgt cactgacatc acgccaggaa
     5581 ttacagagtt aatcagtcat actgacatat ccagattcag tgaaatcgcg caatttcagt
     5641 catcacagca aaccttattt accgacctgc cactaattga aaaagaaagc tgggagacat
     5701 ctttcattcc ttttacacca gaacaacaaa tcctttggca attaggtttt aatgaatggc
     5761 tgcattgcga agatgactta catgaaaaaa aatatatacc gcaaagcgct gttgatgcgt
     5821 tgttgcgctt tgattttcca gcactaaaag ctgagtttgc gaaatatcac aacaatgcca
     5881 acaaaagctg gaatctatct gcgctttgtt acttaccggg acaacaggcg atttcatttc
     5941 tcaaccaaat aatcattgag gagcgatatt caggtgagaa agagattctg gcggtttttg
     6001 gtagtacagc cattcctgcg tttatgacct gcttgcaacg agatcatcaa cgactgtgga
     6061 tattcaccct ctttattggg gccagtgaac tagccttacc aatggcgcag cggttacaga
     6121 aaaaaatggc ttacaaggat gccgttaatt ggctggcaaa taacccgcgc catgccaccg
     6181 ccgggctact gcctttggcg ctgggcaaac cgtgccaaaa tcgggaatat gcccgccagg
     6241 cactccgcct gctagtgaaa ttaaaccagc gagaaacgat tgaagaaatc gcccggagat
     6301 acaaccaacc tgatgtttta gctgcgctgg caacattgtt cgatagcgat ccgctggaag
     6361 aatatcccgc taaaatcgcc ccaccgcccg gcttttatca gttcacctta tggcgcagac
     6421 cacggcttaa aagtaacaac ctgcctctgc cagatgacgc tatgcgccac ctaggcacaa
     6481 tgttgagctt tccacgcgac attaccgcct acgctgggct ggcgaccatc aaagagacct
     6541 tcacccgcga atcactggct gactttggct gggatctgta taccgcatgg acagaagctg
     6601 gcgcacccgc aaaagaaaac tgggcattta cttcgctggg gattttaggc aatgacgaca
     6661 ccgcgcgtaa gttaaccccg cttatccgcg cctggcctgg ggaatcccag cataaacggg
     6721 cggtgtccgg gctggatgta ttagctgata ttggcagcga tgttgcgcta atgctactta
     6781 atggcattgc gaaaaagatt aaattcgttg cattacagga acatgcccgc gagaaaatca
     6841 acatagttgc tgaaaaccgt gggctgacta tggctgaact ggaagaccgc ctggctccag
     6901 atttagggct tgatagcagt ggctcgctga cacttgattt cggcccacgc caattcaccg
     6961 ttggttttga cgaaacctta aaacctgtag tgcgcgatgc aaacggaaag gtcctgaaag
     7021 atttacccaa acaaaaccag agcgatgata aaacactggc aactgacgcc gttaacctct
     7081 ttaaacaatt gaagaaagat gtgcgcgcca ttgccagcca gcaggttgat cgtctggagc
     7141 aggctatgtg ccagcgcaga cgctggacgg cagaacagtt ccgcctgttc ctggtggagc
     7201 atccgctggt acgtcactta acccgacggc tgttatgggg ggtatatact gaggaaaaca
     7261 cgctgattgc ctgcttccgg gtcgcagaag acagcactta cagcgatgcg caggatgagt
     7321 tattcacgct gccagcagga aacatcggta taccgcatgt gctggaaatc ccccctgaat
     7381 ccgccgctgc attcaggcaa atttacgttg attacgaact gcttccccct ttccaacagc
     7441 tcgaacgtgg tagttaccac cttgctgata acgaacgtaa tgtccatgaa ttgtcacgct
     7501 gggacggacg gctctgccag gctggtcgca ttgtcgggct ggaacgcaga ggctggcaac
     7561 gtctggaaga aagcggcagc gtctatgcga tgcgcaaatc taccccttat ggcgctctcg
     7621 aacttgaaac agaacatttt tcattaattt atggcgaaac ggggtatagc gacctgctgc
     7681 ctgtcgaaag cgtaaaaatc actgcctctt atgatcgcta cggtaaacaa tcctccccca
     7741 ctttctccgt gctcgacaac atcaccgcta gcgagctgat taacgatatt gaatcactgt
     7801 ttgattaaaa ggatttagca atgaaaacct atatttatca ggacgaaaaa tcacataaat
     7861 tctgggcggt ggagcagcag ggaaacgagc tgcatattag ctggggtaaa gtcagcacca
     7921 acgggcaaag tcaggtaaaa agctttgctg atgctgcggc ggcggaaaaa gcggaactga
     7981 agttgattgc ggagaaaaca aaaaagggct acgtagaaga agtatccgtg ataacaccga
     8041 catctgtccc cgtccaggtt atcgaatgcc ccgaaatagc ccctttgcca caagacaaac
     8101 cagtatttgt tggagacaac ctcccctggc tggcagacga cgcccaaatc attttaccga
     8161 cagaggttgc gccaaccaca ctaagtcatc gacgctggcc tggcgatcct gtgccacagg
     8221 agaatgaatt aactctgcta cgctccgtcg ccgcgaatac tcaccgacgt tttaaaaagg
     8281 tgatcacctt cgattacagc acctgttcac tggactggca gcaggcgata acacaagcag
     8341 tgggccttat cgactcgccg attagcacca cgctaccacc gatggttctg gcggtactgg
     8401 ttgcgctgga acaaggcttc aatcgtaacg atcacgaaga gttaatggat caaattgtcc
     8461 aggaaggtgg tctggaatat gccactgagg ttgtcattgc actgcaattc atccgcttcg
     8521 actgggatta tgatgcccat cttattacgt tcaccccgga tgacaggcag cctggctatc
     8581 tgttgcgctt tgcctcggtt gaaatgcgcc tgcgtaagca tttctcactt gcaaatgacg
     8641 atgtatggca gcgctgcgcc gacaaactga ttgccgctct cggaaacatg cccgcctggc
     8701 accagccgct ggttgcacta ttgttgccgg aaaaaccaga agtagctcat gagattgcca
     8761 gacatttttg cgggcaaaaa gggttatatg cgctggaatg gctaaaactg acggtcaccg
     8821 atgctcaggt attagcagac ctggaaaaat actatccagg acagcctggg caggtatttg
     8881 atgattacta tggcggtaac atctggtgtg cgacagcact ccaggagcaa ggagccactg
     8941 cgctggcccg ttttgcccat tacgctactg gtgacacatg tggtgaagtg ttgatgcata
     9001 tcaaccaccc gcaggcactt acactactta tccatgcaag cgagcaagga aagcgttgcc
     9061 acgatcgcat gacaaaggct ttcgttcgct tcccacacgc agcgctggcg gcattagcag
     9121 aacttctggc acaaaaagat gaaaagcgct ggcgcatgat gctaatgacg atgcttatct
     9181 cgcaaccgat actagcagaa caggtcattc cctggctctc gacacccgca gttgccgtac
     9241 tgaaatcatg ccagcaacaa ctgaaacagc cctcaaacca tgccagcgcc gatatgctgc
     9301 cagccatatt agtctcaccg ccctggcttt cgaaaaagaa aaaatctgta atgccggtat
     9361 tggatttaac gccattaccg ctggagtcat gctgcacatt gaccgagacg gcagaaaaag
     9421 agattcatgc aaggcatcgc tggcacgcac atcaaataga catcggccaa aaagaggata
     9481 ttcagaacta cctgacacgt ctgggtttca atcgctggaa taatggccag tatatgaaag
     9541 cgtctgatgc tgttgtagaa ctctggcaga ggggagatta ttctgcgcta atcagcgaat
     9601 ttaaaacctt ctggcactcc tatcaacggg aatggcaact ttacatgtta gccgccctgc
     9661 ctatcgaaaa aacagcgcag gcgtggaatg tcctcagtaa agagccacac gtcggtgtcg
     9721 agtttgtcat gacgcacctg caactcgctg ggttacaggg ttttattcac tccttttcac
     9781 gctatccaca agaagctctg cctgtagcac aatattttgc ggctatcgaa ttagcgccac
     9841 tgatcgcccg cgccttcaac aaactcaaaa ccctacgaca agacgcccgt agctggctgc
     9901 tgaaataccc ggaacacgcc attaccggcc tgctgcccgc ggcgctcggc aaagccggtg
     9961 aagcccagga taacgcccgc gctgccttgc gtatgcttac cgaaaacggt catcagccat
    10021 tactgcaaga aatcgcccgg cgttataacc agccggaagt aaccgatgcg gtgaacgctc
    10081 tgcttgcgct cgatgcctta gataatcacc cgacaaaaat ccccactctt ccggcctttt
    10141 atcagccatc gctctggacc cgcccggtat taaaagcaaa tgcccaatca ctgccagata
    10201 gcgccctcct acgcctcggt gaaatgctcc gcttccctca ggaagaggca ctgtatccgg
    10261 gattattgca ggtgaaagcc gcctgtaccg ccgactcact ggctgaattt gcctgggatc
    10321 tgtttaccgc ctggcagacc gctggcgcac cgtctaaaga aagctgggca ttcactgcgt
    10381 taggtgttct cggcaacgat gacaccgccc gcaaactgac gcctttgatt cgcgcctggc
    10441 ctggcgaatc ccagcataaa cgcgccaccg tcgggcttga tatcctcgcc gctatcagca
    10501 gcgatatcgc cctgatgcag cttaacggca tcgcccagaa actgaaattc aaagccttac
    10561 aggagcgggc gaaagagaaa attgccgaca ttgccgagag ccgcgaactc acggtggcgg
    10621 agcttgaaga tcgcctggcc ccggatctgg gactggatga taacggttcg ctactgctgg
    10681 atttcgggcc acgtcagttc accgtcagct ttgatgaaac cttaaaaccg tttatgcgcg
    10741 atgcttccgg cagccgcctg aaagatttac ccaaaccgaa caaaagcgat gatgaatcgc
    10801 ggtcgaacga tgcggttaac cgctacaaat tgctgaaaaa agatgcgcgt accgtcgccg
    10861 cccagcaggt agcaaggctg gaatccgcca tgtgcctgcg ccgccgctgg tcgccggaaa
    10921 actttcagct cttcctggtt gagcatccgc tggttcgtca cttaacccgc cgtctgattt
    10981 ggggcgttta tagcaccgaa aacgagctac tggcttgctt tcgcgtggcg gaagataaca
    11041 gctacagcac cgcagacgat gatcttttca ccctgccgga aggcgatatc tctgttggta
    11101 ttcctcacgt tctggaaata tcacccacgg atgctgccgc ctttggtcag ctttttgccg
    11161 actacgaact gctaccgccg tttcgccagc tcgaccgtaa cagttacgcc ctgacagaaa
    11221 ccgagcgcaa tgccagcgaa ttgatccgct gggcaggcag aaaatgcccg agcggtcggg
    11281 tcatgggact ggcgaataaa ggttggataa agggcacccc gctggatgca ggctggatcg
    11341 gctggatgat caaacctctg ggccgctggt cgttaatcat ggaaatcgat gaaggttttg
    11401 cggtcggcat gtcgccagcc gaactcagtg ccgagcaact cttaagcaag ctgtggctat
    11461 gggaaggcaa agcggaaagc tatggctggg ggagtcattc aacacaggaa gcgcagttct
    11521 ccgtgctcga cgacatcacc gccagcgaac tcattaacga tattgaagcc ctgtttgaat
    11581 aaggaaagag catggacaag gaattaccgt ggctggcgga taacgcccaa gtggaactga
    11641 aatataaaaa aggcaaaacg ccgctcagcc atcgcagttg gccgggcgaa ccagtgcctg
    11701 ttatcactga aagcctcatc cagacactgg gtgatgaatt gctacaaaaa gctgagaaga
    11761 aaaaaaacat tgtctggcgt tatgaaaatt tttcactgga gtggcagtcg gccattacgc
    11821 aggccatcaa cttgatcgga gaacacaaac cctcagttcc ggcccggaca atggcggcgc
    11881 tagcctgtat cgcgcaaaat gatagccaac agttgctcga cgaaatcgtc caacaagagg
    11941 gactggaata tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaaa
    12001 gtgatcctct gctagtgaca ttgcagtatc agaacgagga ttatggctat ggttatcgat
    12061 cagaaaccta taacgaattc gatctccgac tgcgtaagca tctctctctg gcagaggaaa
    12121 gcagctggca gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc
    12181 gccgcccttt tattgctctg atcctcccgg aaaaaccaga aatcgccaat gagttggtaa
    12241 gccttgaatg cccgtggact cattttcatt ctaaggagtg gttaaaagtt gttgctaatg
    12301 accccagagc ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcggg
    12361 aagccagcta catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc
    12421 gcgaacaagg actggcagcc gttccacgcc tggcaatgta tgcccataaa gaagattgcg
    12481 gcagtctgct ggtacaaatt aaccatccgc aggtcatccg catcttgctg ttagttgctg
    12541 ataaaaacaa acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga
    12601 cgcttgccgc actggcagag cttctggcgt taaaagaacc gcctgcacgc cctggttatc
    12661 caatcatcga agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta
    12721 cgctgttaca aacgctgatg gcattgcagc cacaactggc agaagaggtg atgccgtggt
    12781 taagtactca agcccgggca gtgctgaata gttttttatc ggcaccgtcc aaaacggtta
    12841 ttgatagcac cgataacagc caaatgcctg aaatcctcgt ttcaccaccg tggcgtagta
    12901 agaaaaaaat gacagttcca cgtcttgatt tggcaccgtt cgaattaacg ccgcaagttt
    12961 actggcaacc aggcgaacga gagaggcttg ccgccactga gtctgcccgt tatttcagca
    13021 cggaatctct tgcggaacgc atggaacaaa aaagtggacg agttgtatta caggagctgg
    13081 gttttggaga tgatgtatgg ctgtttttga attatatact ccccggaaaa ctggatgctg
    13141 catgcaattc actcattgtt cagtggcatt actacccagg gcgggttgaa gagatcatga
    13201 atggctggag ctccccggaa gctcaattag cagaacaggc gcttcgcaac ggtcacgtag
    13261 aagtgttaat taacatatgg gaaaatgaca gctactcacg ctatcgtcgg gaaaagagta
    13321 tctggaacct gtatttattg gcgcagttgc cgcgtgagat agctttgact ttctggctac
    13381 gtatcaatga gaaaaagcat ctgtctgcgg gtgaggacta ttttctcagt atcttcggat
    13441 tggatgctct accaggtctg ctgttggctt tttcacatcg tccaaaagaa acatttccat
    13501 taattttaaa tttcggcgca accgaactgg ccctgcccgt tgcccgcgtc tggcgacgtt
    13561 tcgcggcgca gcgtgatctg gctcgccagt ggattttaca ttggccggaa catacagcta
    13621 ctgcacttat ccctcttgtc ttcaccaaat ccagcgataa tagcgaagct gcattacttg
    13681 ccctgcgttt actttacgaa caggggcatg gcgagttact gcaaaccgtg gcaaaccgct
    13741 ggcagcgtac agatgtatgg cctgccctgg agcatttact taaacagagt ccaatagaga
    13801 tttacccgac acgcattcca aaagcccctg atttctggca gccggcaatg tggtccaggc
    13861 cgcgccttat cacgaataat cagcctgtta ctgatgacgc tctggaaatt atcggcgaaa
    13921 tgctgcgctt tacccaaggc ggacgttttt attgcggact ggaacagctg aaaacgtttt
    13981 gccagccgca aacgttggca gcctttgcct gggatctttt caccgcgtgg caacaagctg
    14041 gtgctcccgc aaaagacaac tgggcatttc tggcgttaag tctctttggc gacgaaagca
    14101 cggcacggga tctgacgaca ctgattctcg cctggccaca agagggcaaa tctgcccgtg
    14161 ccgtcagtgg tctgaatata cttactcaga tgaataatga tatggcactg atacagctgc
    14221 atcatatatc gcaacgggcg aaatcccgcc ccttacgtga taacgcggcg gaatttcttc
    14281 atgtggtcgc agaaaatcgc ggtctaagcc aggaacagtt agcggacaga ttagtcccaa
    14341 cgctgggtct tgatgatccg caggcgttga tttttgattt tggtccacgg caatttaccg
    14401 ttcgcttcga tgaaaacctt aacccggtta tctttgatca gcaaaacgtt cgccagaaaa
    14461 gcgttccccg gttgcgcgcc gatgacgatc aactgaaagc acccgaggca ctggcccgac
    14521 taaaagggct aaaaaaagat gctactcagg tgagcaaaaa cctgctcccg cgtcttgaaa
    14581 ctgccctgcg caccacccga cgctggtcgc tggcagattt tcattctctg tttgttaatc
    14641 atccctttac ccgcctggtt actcagcgat tgatatgggg tgtttatccg gcaaatgaac
    14701 cgcgttgttt actcaacgcc tttcgtgtgg ccgctgaggg ggagttctgc aatgagcaag
    14761 atgagccaat tgacctgcct gccgatgctc tgattggcat tgcccacccg ttagaaatga
    14821 cagcagaaat gcgcagtgaa tttgcacagc tttttgccga ttacgaaatt atgccgcctt
    14881 ttcgccagtt ggcacggcgt acggtgctgc tcacacctga cgagtcagcc agtaatagtt
    14941 tgaatcgctg ggaaggtaaa tccgctaccg ttgggcaact gatgggaatg cgatacaaag
    15001 gctgggagtc aggctatgag gacgcatttg tctatgacct gggtgcgtac cggctggtcc
    15061 ttaagttttc acccggtttt aaccactaca atgttgatag caaagcgcta atgagcttcc
    15121 gttctcttca tgtataccgt gacaataaat ctgtcacttt tgccgaactt gatgtgtttg
    15181 atttgagtga ggcgttaagc gcacccgacg tcattttcca ttaaaacaca acgggcagtg
    15241 atgcgactgc ccgttatcaa cacgacttac catcgggaaa agcgatgagc gtgcaaaaag
    15301 agctggtcgc tatttacaat tatgaggtcc ctgtacctga agatccgttt tccttcagac
    15361 ttgagatcca taaatgctct gaattattta caggttccgt ctaccgactg gaacggttcc
    15421 agctacatcc aacatttaat gatgccgacc cgctaataaa tgatgcgttg atttatataa
    15481 gagatgagtt tattgatgag cggaaattac gaggtgaatc acctgaaacg gtaatagcaa
    15541 tttttaatcg cgaactacag aatatattca accaagaaat agaaaaatag ctttattaaa
    15601 gggggaaacg gtattccccc ttagatgcgt ttcaatttgc ccgttacttc aacaaatcaa
    15661 tcaaccgtaa cagagccact aataactcca gtacggcaat gagcacggtt aacttttgtg
    15721 tcagcgtgtc catccgctct ccttattaag gagcgcaaca ccagcacctt acccttacgc
    15781 tgcctgtcga tactggttta cgtatgttga gtcgccatgt tgcactccgg atcagagcac
    15841 tacctgcggc accacccgtc cggtagccag gacttgaaat gggttcgatc cggcgcaact
    15901 tcccaacagt caggattata aatctcatta tcttaattgc aacactgtcc agaggtggtt
    15961 ataattcgtt cgttgagatg ccatgttgca ctatcagtaa cttaacatca aagtagtaaa
    16021 taaaagaacc tgcctcacca gcaggttttt ttattttctg cgatctactt tccagatatt
    16081 ttttgccaat agcccaccat aaatattcac atcccggtta aacggtacgt tgaatataaa
    16141 atcgatgaat ttataaataa aaatgagtta tatgatgaat taataaaaac agtaatcatt
    16201 ttattaaagg gggaagcggt tttccccctt agatgcgttt caatttgccc gttacttcaa
    16261 caaatcaatc aaccgtaaca gagccactaa tagctccagt acagcaatga gcacggttaa
    16321 cttttgtgtc agcgtgtcca ttcgctctcc ttattaagga gcgcaacacc agcaccttac
    16381 ccttacactg cctgtcgata ccggtttacg tatgttggac cacgatgttg cactccggat
    16441 cagggcacca cctgcagcac cacccgtccg gtagccagga cttaaaattg gtccgatccg
    16501 gcgcaacgtc ccaaccgtct ggattataaa tctcattatc ttaattgcaa cggggtccac
    16561 cgatggttat aattcgttcg ttggaacacg atgttgcaca ggctgcggtg ttgggcctga
    16621 aaatagtaaa taaaagaacc tgcctcacca gcaggttttt ttatttactg tgatctgctt
    16681 tccagatatt tttcgctcaa acaactaatg cgccaaacat ttattgcgcg taaaatatcg
    16741 tttatttcat taatacattt cagggatgaa tatatgtcac cgcagaataa tcatctgcag
    16801 cgtccgcctg ctgctgtgtt atacgccgat gaactggcaa aattaaaaca aaatgataac
    16861 gctccttgcc cgcccggttg gcagttaagt ttacctgcgg cccgtgcttt tatccttggc
    16921 gatagcgcgc aaaatattag ccgtaaagtg gtgattagtc cctccgctgt cgaacgtatg
    16981 ttagtcactc ttgccaccgg gcgcggtttg atgttggtgg gggaacctgg caccgcaaaa
    17041 tctctcctgt ctgaattact ggcaaccgct attagcggcg atgccgggtt aaccatccag
    17101 ggcggcgcat ctactactga agatcaaatc aagtacggct ggaactacgc cctgcttatc
    17161 aaccatggtc cgtcaacgga agcactggtc cctgccccgc tttaccaggg aatgcgcgac
    17221 ggcaaaatcg tccgttttga agagataacg cgtacaccgc tggaagtaca agactgtctg
    17281 ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga agccagtcaa
    17341 ctctatgcgc gagaaggctt taacattatt gccactgcca atacccgcga tcgcggcgtc
    17401 aatgaaatga gtgctgcgct aaagcgtcgt ttcgattttg aaactgtctt tccgattatg
    17461 gatttcgctc aggagctgga gcttgtcgcc agtgcttcgg cgcgtttgat ggcccatagc
    17521 ggtattccgc ataaagtgcc tgacgcggta ctggagttac tggtccgcac cttccgcgat
    17581 ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac tgacagcgat tatgtccacc
    17641 gctgaagccg tgaacgtggc ccacgcagtg ggcgtccgcg cctggttttt agcgaatcgc
    17701 gcgggggagc ctgccgacct ggtggactgt attgccggaa ccatcgtcaa agataacgaa
    17761 gaagatcgcg cgcgtctgcg ccgttacttc gaacagcgcg tcgccacaca taaagaagct
    17821 cactggcagg cttattatca agctcgccac cgcttgccgt gaggaaagat gcatgagcga
    17881 gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg tgaaatcgtt
    17941 aatcgaaagc cagcggccac gatacgtgtt gattgaaggc ccggctgatt ttaatgaccg
    18001 ggtagacgaa ctgttgttag cccaccagct tccggtagct atttacagtt attgccagta
    18061 tcaggacggt gcggcccccg ggcgtggtgc ctggacgcca tttgctgaat tttcgccgga
    18121 gtggcaggcg ctacaagccg cacgtcgtat tcaggcacaa acttacttca tcgatttgcc
    18181 ttgctgggca cagagtgaag aagaggacga ttcgcctgat acgcaagagg aaatccagac
    18241 cttactgctg cgtgccaccc gcatggataa cagcgatacc ctgtgggatc acttattcga
    18301 agatgaaagc cagcaaacta tattaccctc tgcactggcg cactattttg cacaactgcg
    18361 gggcgattcc cccggagatg ctctcaatcg tcagcgcgaa gcctttatgg cccgctggat
    18421 tggatgggcg atgcagcaaa ataatgggga cgtgttagtc gtctgcggcg gctggcatgc
    18481 tccggcactg gcaaagatgt ggcgcgaata cacgcaggaa attaacacgc cggaattgcc
    18541 ctcgctggca gatgccgtta caggttgtta tctcaccccc tacagtgaaa agcgccttga
    18601 tgtgctggca ggataccttt ccggtatgcc tgccccggtc tggcaaaact ggtgctggcg
    18661 gtggggcttg cagcaggcag gtgaacaact gctgaaaacg gttcttaccc gtttgcgcca
    18721 gcacaaattg cccgcttcta ccgctgatat ggctgccgct catctgcatg cgatggcgct
    18781 ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg ccatagcagg
    18841 ctcgctgatt aaagaagcct tgaacgcgcc gttgccgtgg agctatcgcg gagttattca
    18901 tcccgatacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg acggattcgg
    18961 taaacttgcc ccctctacgc cacagccgcc tctgccaaaa gatgtcacct gcgaactgga
    19021 acgtacagga atctcttttc cggcggagct taccttaaat cgctttaccc ccgatggact
    19081 ggcgcaaagc caggtgctgc atcggttggc aatactggag attcccggca ttgtgcgcca
    19141 gcacggaagt acactgacgc ttgcgggtaa cggtgaagaa cagtggaaat taacccagcc
    19201 gcttagccag catgcggcat taattgaggc cgcatgcttt ggcgccacac tccaggaagc
    19261 cgcacgtaat aaattagaag ccgatatgct ggacgcgggt ggaatcggca gtatcaccac
    19321 atgtcttagc caggcggcgt tagcgggtct ggcgtccttc agtcaacaat tactggagca
    19381 acttacatta ttaatcgccc aggaaaatca gtttgccgaa atgggccagg cgctggaagt
    19441 gctatatgcc ttatggcggt tggatgaaat tagcggtatg caaggcgcgc agatattaca
    19501 aacgacatta tgcgcggcca tcgatcgcac actctggctg tgtgaatcca acggcagacc
    19561 ggaggaaaag gagtttcacg ctcacctgca tagctggcag gcgctttgcc atatcctgcg
    19621 cgatctacat agcggcgtta atttacccgg cgtttcgctt tctgcggcgg tggccttact
    19681 ggagcgctgc agtcaggcgg ttcatgcccc ggcactggat cgcggcgcgg ctcttggcgc
    19741 actgatgcgt ctggaacatc ccaacgccag tgccgaagcg gcgctgacga tgctggcgca
    19801 attatccccg gcacaatccg gcgaggcgct gcacggtttg ctggcgctgg cccgccatca
    19861 actggcctgt cagccggcat ttatcgccgg tttcagtagt catttaaatc aactgagtga
    19921 tgccgatttt attaacgccc tgcccgattt acgcgcggcg atggcctggc taccgccacg
    19981 agaacgcggg acgctggcgc atcaggtgct tgagcattac cagctcgcac agcttcccgt
    20041 ttcagcgctg caaatgccgt tgcattgtcc accgcaagcc attgcacatc atcaacaact
    20101 cgaacagcag gcactgggat cgctgcaaca ctggggagtt ttccatgtct gaactgaacg
    20161 atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa
    20221 cgacgctttg tgggctggat ggcaatgccc ggcaaataga tcacgcgctg gagtggctgt
    20281 atgggcgcga tcctgaacgg ctccagcgtg gtgaacgctc cggtggatta ggtggctcaa
    20341 atctcactac ccctgagtgg atcaacagta ttcacacgct gtttccgcaa caggtgattg
    20401 agcggctgga aagcgatgcc gtactgcgct acggcattga agatgtggtg acgaatctcg
    20461 acgtgctgga acgtatgcag ccttctgaaa gcctgctacg cgccgttttg cacaccaaac
    20521 atctgatgaa tcccgaagtg ctggctgccg cccgccggat agtgcgccag gttgttgaag
    20581 aaattatggc gcgactggca aaggaagttc gccaggcttt ttctggtgtc cgcgatcgcc
    20641 gccgtcgctc atttattcca ctggcgcgta atttcgattt caaaagtacg ctgcgtgcca
    20701 acctgcaaca ctggcatccg caacacggca agttgtatat cgaatccccc cgctttaaca
    20761 gccgtattaa gcgccatagc gatcaatggc aactggtctt actggttgat caaagcggat
    20821 cgatggttga ctcggtgatc cactcagcgg tgatggcggc ctgtttatgg cagttacccg
    20881 gcattcgtac ccatctggtg gcgtttgaca caagcgtcgt tgatctcacg gcagacgttg
    20941 ctgatccggt agagttatta atgaaagtac aactgggcgg cgggaccaat atcgccagtg
    21001 ccgtggagta tggtcggcaa cttattgaac aaccagcgaa aagtgtcatt atcctcgtga
    21061 gcgattttta tgaagggggt tcatcatcca tgctgacgca tcaggtgaaa aagtgtgtcc
    21121 agagcggcat caaagtgctg ggactggcgg cgctcgatag caccgcaacg ccttgctatg
    21181 accgcgatat ggccctgacg ctggttaatg tcggcgcaca aatagccgcg atgacgccgg
    21241 gcgaactcgc tacctggctt gcggagaatt tgcagtcatg aattcactac gtccggaatt
    21301 attagaactc acgccgcagg ccctgacggc gttaagcaat gccggttttg ttaagcgcag
    21361 tcttaaggaa ctggaaaatg gcaacgtccc ggagatcagc catgagaacg gcgctttaat
    21421 cgccaccttc agtgacggtg tccgcaccca gctggcgaac ggtcaggcac tgaaagaggc
    21481 tcagtgcagc tgcggggcca gcggcatgtg ccgccatcgc gtgatgctgg ttttaagtta
    21541 tcagcgactt tgcgccacca cccagcctac ggaaaaagaa gaagagtggg atccggcaat
    21601 ctggctggaa gaactggcta cccttcccga tgccacccgc aaacgcgcac aggcgctggt
    21661 cgctaaaggc atcaccattg agttgttctg tacgccgggc gaaattccct ctgcccgctt
    21721 accgatgagc gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc gttgtgattg
    21781 tattgacggc acactttgcg aacatgtcgt actggctgtg caggccttcg tcgaggccaa
    21841 aactcagcaa gcggaattta ctcatttaat ctggcagatg cgcagcgaac acgtcacacc
    21901 atctgacgat ccgtttgcca gcgaagaggg caacgcgtgt cgtcaatatg ttcagcaatt
    21961 aagtcaggca ttatggctgg gcggcatcag ccagccgcca atccattacg aggccgcttt
    22021 cagccgcgca cagcagacgg cggaacgctg caactggcga tgggtgagtg aatcgctacg
    22081 gcaactgcgc gccagcgttg atgccttcca cgcccgcgcc agccactatc atgccggaga
    22141 atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag agatggcccg
    22201 gcgcgacagt atcggtgaag ttcctcctgt accgtggcgc acggtcgttg gcgctggcat
    22261 tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc gttgctggca
    22321 ggatattgag cagtatggtt tacgcatctg gttcaccgat cccgacaccg gcagtatttt
    22381 gcatctttcg cgtagttggc cgagaagcga acaagaaaac gctcccgctg caaaacgtcg
    22441 gctgtttagt tttcaggctg gcgcactggc aggtgggcaa attgtttcac aagcagcaaa
    22501 acgcagtgcc gatggcgaac tgctattagc tacccgcaac cgcttgagta gtgttgttcc
    22561 actgtcgcca gacgcctggc aaatgttgag tgcgccgctg cgccagccag gcattgtggc
    22621 tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cgaccactca accaggtcga
    22681 taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata gcagccgcca
    22741 gacactggat gcgcaggtca ttagcggcga aggggaagat aatgtgctga cgttatccct
    22801 tccggcatct gcctgttctc cttttgccgt tgaacgcatg gcggcgcttt tgcaacaaac
    22861 agacgacccc gtgagtctgg tttctggctt tgtcagcttt gtagaggggc aattgacact
    22921 ggaaccacgg gtgatgatga caaaaacccg cgcctgggcg ctggacgcag aaactgcgcc
    22981 tgtggcaccg ctaccttctg ccagcgtttt gcctgtgccg tctaccgctc atcagttgct
    23041 gatgcgctgc caggcgttac ttattcaact gctccataac ggctggcgct atcaggaaca
    23101 gagtgctatt ggtcaggcag agttgctggc gaatgacctc accgcggtcg gtttttatcg
    23161 gctggcacat gtgttggcac aatttcgtaa tacagaaagc gaggcacggg tagaagcaat
    23221 gaataacggt gttttgattt gcgaacaatt attccccatg cttcagcaac aaggatgaaa
    23281 tagtgctttt tactaagagt tccactccag ttccggactg ctcacgccac ggtattaggc
    23341 atatcctata tagcccctgg tgagagtcac cagtttcttg attaaataaa atggagtttt
    23401 acatgaaggc tttcaataag ctgttttccc tcgttgttgc atctgttctg gttttctctc
    23461 ttgctggctg cggtgacaaa gaagaatcga aaaaattcag cgccaatctg aacggtactg
    23521 aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct tctgaaacca
    23581 aaattcaatt tgcctccatt ggtgcaacca ccaaagaaga tgctgccaag acacttgagc
    23641 cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc tataccgata
    23701 cttacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa gccctgcagg
    23761 gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact atggcgcaaa
    23821 tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatctgtc ggcggccatg
    23881 tttttgcatg gccgccatac ccgtcttagc ttttcttcac atgctggcgc gctgccagtc
    23941 cacgcagaaa ataacgtaaa aattgatcgc cgcattcgcg gaagttttta tgatccggtg
    24001 cacgcatcat cgccgtaatt tccggcatcg aaacgcggaa ctgctgttcg gtaaggatcg
    24061 ccagaatgtc atcggttttc agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt
    24121 tattaatgcg acgttccggc tccagtgccg gagcagactc atccttgccg cgtttttcat
    24181 aaatcaggcc attgaggaat gacgacaaaa caatgtccgg acaacgctga aaaccctctt
    24241 cgtcttcttt acgtagccag acggcgatct gttccgcggt ggcttcgaca ttacccagcg
    24301 ccagaatacg caccaggtca ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa
    24361 tatcgttact tagcatgagg ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc
    24421 ttttaaaggc caatcgcctc ttttaagctt ttcagataac ggcggctgac cggcacggtt
    24481 aagccattac gcaaaatcaa ctcggcctgg ccgttatctt ccagacgaat ctcctgtaaa
    24541 tgcgcgaggt taaccagata ctgacgatgg cagcgcagta gtggtgtacg actttccagg
    24601 gtacgtaatg tcaattcggt aaagccctct ttcccttcgt ggctggtaac gtagacaccg
    24661 ctcatccgac tgctgacaaa tgccacatct ttcatttgca gcaaataaat ccgactatgc
    24721 cccgtacaag ggataaactt cagcgcctgt tgattttccg gtaacagcga aacatcctgc
    24781 ttgctgcgct cctgacgcaa tcgcgccagc gttttttcca gtcgcgcttc atcaattggc
    24841 ttcagcagat aatcaaaggc atgttcttca aaggctttaa tagcgtattc gtcaaacgcg
    24901 gtgagaaaaa cgatatacgg tcgatgttcg gggtcgagca tccccaccat ttccagacca
    24961 ctgatgcgcg gcatctggat atcgagaaac agcacatccg ggcgcagttt atgcaccgcg
    25021 ccgatccctt ctacggcgtt tgaacactct ccaacgattt caatatcgct ctgctcctgc
    25081 aaaaatacgc gcaggttctc ccgtgcaagc ggttcatcat cgacaattaa gactttaatc
    25141 atgcctcgtc cctccatggt agtcgtaacg ttattcgggt gtaacaatca ggctcacagg
    25201 cgacgcttat cccatagtca tcgccaaacc gttcacgtaa acgcttatcc accagattca
    25261 tccccagccc actggcattg gttaccggtt gatacaaacc ggcattgtct tcgatctcca
    25321 gcatcaaatg ttgcccctca cgtcgggcgc tgatcgccac tcgccctgta tccagcagtt
    25381 gtgatgtccc atgtttaatg gcgttttcca ctatcggttg cagggtaaac gcgggcaatt
    25441 gctgctggga taattcttgc ggaatagcaa tattgacctg caaccgcgac tggaagcgcg
    25501 ccttttcaat ttgcagataa gcgttcacat gttcaatttc gtcggcgaga gtaacaaact
    25561 ccgaaggccg ctttaagttt ttgcggaaaa aagtggaaag atactgcacc agctggctgg
    25621 cctgttcgct gtcgcggcgg atcaccgctt taatggtgtt aagcgcatta aacaaaaaat
    25681 gggggttcac ctgggcgtga agcagtttga tctctgactg ggtgagcatc gctttttgcc
    25741 gctcatattg cccggcgagg atctgcgccg aaagcagttg cgcaatcccc tcacccagcg
    25801 tgcgattgat tgaactgaat aaacggtttt tggcttcata caatttgatg gtacccatca
    25861 cccgctgatt ttcaccacgc aacggaatta ccagcgtcga ccccagtttg cattgcggat
    25921 gcaaagagca acgataaggt acttcgttgc catcagcgta gaccacttca ccggtttcaa
    25981 tcgcttttaa ggtgtaagtc gaagaaatcg gtttgccggg taaatggtga tcgtcaccaa
    26041 ttccggtaaa ggccagcaat ttctctcgat cggtaatcgc gactgcacca atatccagct
    26101 cctgatacag cacctgtgcc actttcatgc tgttcacttc gttaaacccc tggcgcaaaa
    26161 tgccttccgt cgaggcagcc acttttagcg cagtggcaga aaaagccgaa gtgtattttt
    26221 caaacatcgc gcgtttatcg agcaatatac gcataaacag cgccgcgccg acggtattgg
    26281 tgaccatcat tggcgcagca atattactca ccagacgcac cgcatcttca taaggtcggg
    26341 cgattgccag gatgatcaac atttgcacca tttcagcgac gaacgtgacg gcaccggcgg
    26401 taatggggtt aaagacttta tcagtgcgcc cgcgacggat caggatgctg tgtaccaggc
    26461 cgccgagcaa cccttcaacg atggtcgaga tcatgcaact tagcgcggtc atgcccccca
    26521 tcgaatatcg atgtaacccg ccggtcagac caaccagccc accgacgacc ggaccgccga
    26581 gtaagccgcc cattaccgcg cctatcgcac gggtattggc aatagaatcg tcaatgtgca
    26641 acccaaacca ggtgcccatg atgcagaaga tggaaaagac gatgtagcag agaaatttat
    26701 gcggcagacg aaccgtgacc tgcattaacg gtatgaataa tggcgtttta ctcattaacc
    26761 acgcaatgac taaaaaaacg cacatctgct gaagcagcag caacaccaga ttaaaatcgt
    26821 acatacccgc aaaccacact tccctttaaa acgcgtaaca tacattgcct gcgtttaact
    26881 ttctttgaac tcttgcagaa aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg
    26941 caaaaataaa atcaccctat agatgcacaa aaaacgggca aaactacctg gttcgcaaaa
    27001 ctgcgtctaa agttaaaccg ggacctcgcg agcaagggtg agacgatggc gctttacaca
    27061 attggtgaag tggcgttgct ttgtgatatt aaccctgtca cgttacgcgc gtggcagagg
    27121 cgttacggat tgctgaaacc gcaacggaca gacggcggcc atcggctgtt caacgatgcc
    27181 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
    27241 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
    27301 gaaacattac tgacttacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
    27361 gagcgcggtc aggattaccc cgcccagacg ctcaccacac atctgtttat tcctctgcgc
    27421 cgacgacttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
    27481 ctgatcaact acatcgccat ttgcctggct tcggcacgta aaaaacaggg taaagatgcg
    27541 ctggtggttg gctggaatat tcacgatacc acccgtctgt ggctggaggg ctggattgcc
    27601 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
    27661 ctgttcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
    27721 cagcaactca ccagttggca ggaacaaggc catgatattt tcccactcgg catttaatca
    27781 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcatcatgc
    27841 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
    27901 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
    27961 ttcaaacagg cggtctgcaa tgatcgccgc cagtgccacc agtaacgccc cctggatcac
    28021 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
    28081 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
    28141 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacggctcat
    28201 tcccatacct ttcgcaactt ctgtcacgct gacatcaatc gctcccagcc cggcaagtgt
    28261 cgcctgcagg acgggcagca cgccgtaaag gatcaaggcg ataatcgcgg gttgcagacc
    28321 aaagccgatc accggaacgg caatcgccag caccgcaacg ggcggaaaag tctgcccaac
    28381 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag taacaataat
    28441 tccggcacca gtaccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
    28501 agccagtgcc agagctgcaa agctttcctg ctgataaacg ggtcgtggca gttgtgggaa
    28561 caaggcagca aacagcggct ggctgtaagg cagccagaaa agcagtgcca caaacaaagc
    28621 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc accagcagat
    28681 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
    28741 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
    28801 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
    28861 ggacacccag ttcactatgc ccaaaaaact ggcggacaaa atcattcgcc ggacgagtca
    28921 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaac accagatgtt
    28981 ctgccagtcg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
    29041 aacggtgtat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagcgcgc
    29101 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
    29161 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgcaaa tttggctcca
    29221 accccagtag cgccattaat tcgtcgatac gatcgtcgat ccgcgcccgt gaccattttt
    29281 gtaattgcgg cacggtggca atattttgcg ccacgctcca gtggggaaac aggccaatag
    29341 attgaatggc atagcccatc cggcggcgca actccagcac tggcagcgag cgaatttctt
    29401 ctccggcaaa gcggatcacg ccattgtcat gctccaccag gcggttaatc attttcagag
    29461 tggtggattt accggagcct gatgtaccaa tcagcaccga aaaactcccc tcctgaaaat
    29521 tgagattgag atcgttaacg gctttttgta tgccgaacag tttgctgaca tggctaaatt
    29581 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc atcgatcagc
    29641 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc actgcttagc
    29701 agcccctgga aaaccagcgc accaaaaccg cctgcaccga ttaacgccgc aatcaccgcc
    29761 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagc
    29821 ggtaactgaa catgcaggaa acgctgcgcc ccgctcatcc ccatcgctct ggcgctctcc
    29881 agcacatcgc gcgggatctg gttcaagccg actaccacgc cgcgcaccag cggcagcaag
    29941 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagctta
    30001 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
    30061 gccaccgaag gcacggtctg aatgacattg agtagcgaaa aaatcgcccc ctgccgcgca
    30121 gtggaaaagt agcaccagac gcccaacggc acaccaatca ctaacgcagg cagcaccgca
    30181 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
    30241 tattctttca ttagcgagag atcgttaagc gcgccggaat acagcaacca cagcggaatg
    30301 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
    30361 gcatcgctac aggccagcaa tgccagcgcc gcagccagcc agaaaccact gccgaggctg
    30421 gtacgcgcca gcgcactgcc attttgtgcc agttgtgtcg ccgcctttcc tgctccccac
    30481 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
    30541 ggaataaaac aggctgtcat ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
    30601 ggccacagct gccagagatg acgcccctca cccgaaacta aacgattagg cgcgtaactg
    30661 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
    30721 atacggaaat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
    30781 gccacttttt tggcatccag tccttccaca gcaatgctgg cattcagttg ctgcaatgtt
    30841 ttttcatcga ggctggcgaa gactggctgt agccactgtg ccatttgtgg atactctttc
    30901 aacaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc ttgcggatcg
    30961 cttaaggttt gcagccccag cgccgcgacc gggccgtcag tgccgtaagc catcgccgca
    31021 ttaacgccag aggtttgctg ggcagcggct ttgatcgtca ccgccgtgtc gccaccagcc
    31081 agtgacagca actgatcctg accgagctta aagccatagg ctttttcaaa cgcgggtaac
    31141 gcatcggcgc gttcaataaa ttctgccgag gccgccagtt tgaaggtgcc gccctctttc
    31201 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
    31261 acggcgatag tccaggtgtt atttgcaggc gcgggcgtca gccagattaa cttgttttgc
    31321 tctgcatcga gttttttgac tttctcgtag ccttgcccgg catttttcca tgccgcatcg
    31381 ttttcatctt taaagaaaaa cgcgccattg ccggtatatt ccggatagat atccagttca
    31441 ccggaagtaa tcgccccgcg caccacggga gtcgttccca gttgcacttt atttaccgta
    31501 ggaacaccgt gactttccag cacctgcaaa atgatattgc cgagcagcgc gccttcggta
    31561 tcgatttttg aaccgacctt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
    31621 aacaaaacca atgaacctgc ccagagcttt gagagtggca tgatgctttc ctcattcttt
    31681 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacagg gttagcctgt
    31741 ctgggcagat tcagttgcag aatcagataa atacttaaag ggcgcaaacg tcgcgccctt
    31801 actaaagcat tacagcaact caaactcgcc tttcttaacg cgtgcggaat cagtgccgat
    31861 gaagacattg aacttgccag gctcggcgtc atatttcatc tgttgattcc agaacttcag
    31921 cgcctcgata tcaatcggga agctgacggt ctgagtttcg cccggtttca gggtgatttt
    31981 ctcaaagcct ttcagctgct tcaccggacg actcatggaa gccgtcacat cctgcaagta
    32041 catctgcact accgtcgccc cttcgcgctt accggtgttc gtcacctgca cactggcggt
    32101 cactttgccg tcacgcttca tggtcggcgc agaaagtttc acatcagaga cggtgaaagt
    32161 ggtgtagctc agaccataac cgaacggata cagcgccccg ttagcttcat caaaataacg
    32221 cgaagtgtat ttgttcggct tgtcggcatt atacggacgc ccggtattca gatggctgta
    32281 gtacacaggg atctgcccga cagaacgcgg gaaggacatc ggtagcttac cggacgggtt
    32341 gtaatcgcca aacaatacat cggcaatcgc attaccccct tccgtacccg caaaccaggt
    32401 ttccagaatc gcatcagcct gctgatcttc tttcaccagc gccagcggac gcccattcat
    32461 cagcaccagc accagcggtt taccagtggc tttcaacgcc gcaatcaagt cacgttggct
    32521 ttgcggaata gtaatatcgg tacggctgga ggcctcgtgc gccatcccct gagcttcacc
    32581 gaccacagcc accaccacat cagattgttt cgcggtttgc accgcttcat caatcatctc
    32641 ttgcggcgaa cgcggatcga ctttgaccgc ctcttcatac tgattcagga aatcgataat
    32701 gcctttgtca ctggtgacgt tcgccccttt ggcgtacagc actttaccgt tttcaccgac
    32761 ggcattttta atcccggtca gtacggtcac ggattgatcg gcaacaccgg ctgcggacca
    32821 gctgcccatc acgtcacgtt tactgtccgc cagtggccca accaccgcaa tggtggccga
    32881 ttttttcagc ggtaacgttt cgagacggtt tttcagcaac accaggcttt cacgcgccac
    32941 ttcacgcgct tctttgcggt gcaggcggct ttcggcattg gtatccaccg ggtcagactc
    33001 tttcggcccc aaatggctgt atgggtcgtt aaacaacccc atatcatatt taacgttcag
    33061 tacatgacgg gtagcgtcat ccagctcttc catcgtcact ttgccggatt tgatcaaccc
    33121 aggcagatac ttcgagtagt actcgtcgct catactcatg ttgatgccgg atttcagcgc
    33181 cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt ttaatcagct ctttgattgc
    33241 gccgtgatcg gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa catctttcag
    33301 cagccatgaa tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca ccatcaccgc
    33361 gccgctgcct gcgtccagcc ccgctttgta cggcggcata taatcattaa acaggcgctg
    33421 cggactcata tcgacggtgt tgtactcttt accgccttct accgcaccgt atgcggcaaa
    33481 gtgtttgacg ctggtcatca ccgagtagcg atctgccgga cttttaccct gcatcgcttc
    33541 caccatggtt ttacccattg ttgaagtgag atacgtatct tcgccaaaac cttcggaagc
    33601 acgtccccag cgcggatcgc gcgagacatc gaccatcggt gcccaggtca tattcaggcc
    33661 atcatctgcc gcttcataag cagagacacg cccgactgtt ttcaccgcat cgaggttaaa
    33721 agacgaggcc aggccgaggc taatcgggaa caccgtgcgc tgaccgtgca gcacgtcgta
    33781 agcaaagaaa agaggaattt tcaggcggct taattccatc acctgatcct gcatggcgcg
    33841 gatatcctga cgggttacgg tgttgaaaat cgccccaacc tgaccgtctt tgatcatctc
    33901 gcggatcgcc tctttcggat tatccgggcc gacgctgatt aagcgcagct gaccaatttt
    33961 ctcatcaact gtcattttct taagcagttc ggtgacgaac gcatcccgcg cttcgggcgt
    34021 taatggatgg ttgccgaata aatcatctgc cagtgctggc tgcagggcca gactcaccgc
    34081 gattcctact gaacatagcc atttcatatg gatttttctt cctctgttgc ccgacgaggc
    34141 agcgaaaata gtgcaaaaac cgtagtttgc cataagcatg atagagagag aaaaagaatg
    34201 ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg aattgtcata caaggcgcta
    34261 ttctagtttg tgatattttt tcgccaccac aaggagtgga aaatgtcttc catgacaaca
    34321 actgataata aagccttttt gaatgaactt gcccgactgg tgggttcttc acacctgctc
    34381 accgatcccg caaaaacggc ccgctatcgc aagggcttcc gttctggtca gggcgatgca
    34441 ctggctgtcg ttttccctgg ctcactactg gaattgtggc gggtgctgaa agcctgcgtc
    34501 accgccgaca aaattattct gatgcaggca gccaatacag gtctgaccga aggctcgacg
    34561 ccaaacggta acgactatga tcgcgatatc gtgatcatca gcaccctgcg tctcgacaag
    34621 ctgcacgttc ttggcaaggg cgaacaggtg ctggcctatc cgggcaccac gctttattcg
    34681 ctggaaaaag ccctcaaacc gctgggacgc gaaccgcact cggtgattgg ctcatcgtgt
    34741 ataggcgcat cggtcatcgg cggtatttgt aacaactccg gcggctcgct ggtgcagcgt
    34801 ggcccggcgt ataccgaaat gtcgttattc gcgcgtataa atgaagacgg caaactgacg
    34861 ctggtgaacc atctggggat tgatctgggc gaaacgccgg agcagatcct tagcaagctg
    34921 gatgacgatc gcatcaaaga tgacgatgtg cgccacgatg gtcgtcacgc ccacgattat
    34981 gactatgtcc accgcgttcg tgatattgaa gccgacacgc ccgcacgtta taacgccgat
    35041 ccggatcggt tatttgaatc ttctggttgc gccgggaagc tggccgtctt tgcggtacgt
    35101 cttgatacct tcgaagcgga aaaaaatcag caggtgtttt atatcggcac caaccagcca
    35161 gaagtactga ccgaaatccg ccgtcatatt ctggctaatt tcgaaaatct gccggttgcc
    35221 ggggaatata tgcaccggga tatctacgat attgcggaaa aatacggcaa agacaccttc
    35281 ctgatgattg ataagttagg cagcgacaag atgccgttct tctttaatct caaggggcgc
    35341 accgatgcga tgctggagaa agtgaaattc ttccgtccgc attttaccga ccgtgcgatg
    35401 caaaaattcg gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa ctggcgcgat
    35461 aaatacgagc atcatctgct gttaaaaatg gcgggcgatg gcgtcggtga agccaaatcg
    35521 tggctggtgg attatttcaa acaggccgaa ggcgatttct ttgtctgtac gccggaggaa
    35581 ggtagcaaag cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg ttatcaggcg
    35641 gtgcattccg atgaagtcga agacattctg gcactggata tcgctctgcg gcgtaacgac
    35701 accgagtggt atgagcattt accgccggaa atcgacagcc agctggtgca caagctctat
    35761 tacggccatt ttatgtgcta tgtcttccat caggattaca tcgttaaaaa aggcgtggat
    35821 gtgcatgcgt tgaaagaaca gatgctggaa ctgctgcagc agcgtggcgc gcaatatcct
    35881 gccgaacata acgtcggtca tttgtataaa gcaccggaga cgttgcagaa gttttatcgc
    35941 gagaacgatc cgaccaacag tatgaatccg gggatcggta aaaccagtaa gcggaaaaac
    36001 tggcaggaag tggagtaaaa attacggatg gcagagtatc gccatccgaa ttcacttaat
    36061 cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt ttatagctca
    36121 acgctgctgc cggaacaggc atcactttac cggtctcaat ccaggtacgc aggcggctgg
    36181 catcggcaaa atgggtatat ttgccaaacg cgtccatcac taccagcgcc accggtttat
    36241 tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag ccggttttgg
    36301 ttaactgaat attccagtta tcgcgataca ccagatggtt agtattgcgg aacggcagcg
    36361 tatacgtcgg gttagagaag gttgccatat cttcccgggt ggtacttaac tgcccgatca
    36421 acggatattg tttgctggca atgagcagtt tggttaagtc acgggcggtt gaaacgttat
    36481 gcaccgacaa tccggtcggt tcaacaaagc gcgtgttgtt cattccgagc gatttcgctt
    36541 tcgcattcat cgccttaata aaggctttgt aaccaccggg ataatggtgc gcaaggcttg
    36601 ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta cggctgattt
    36661 cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg ctgatatcca
    36721 cttttagttt ttcatccagc ggcagtcgcg catccagcac gaccatcgcg gtcattaatt
    36781 tgctgataga cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc actttgttgg
    36841 tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggctgtgaa gcggtggtag
    36901 cggctgccgt tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc agggccaggc
    36961 taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt cacgcgcacg
    37021 ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat aacgagcaaa
    37081 aagtgcgatc gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat ttacgccagc
    37141 aatgcaacac aaaagtactt tctagaacaa acgataaccg tagccccata gtataacggt
    37201 aagggcgagc agcacttcca gcaccagcac gccaatcgcc agcgtcgaac tggagaagct
    37261 aaggccttcc tctttgttga tattcaggaa gctcggaata ccgagataaa gcaggtagcc
    37321 ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac cagaccagtg gataaagcgc
    37381 caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca tgcagtgcgc
    37441 aagtgatgga cgttgcggat aattgcgcgc catccaccag atgacccgcc ccatcaccgc
    37501 gaccccagcc agcataacgc cataaaacaa gacagccagc gccagtccgg taaaccagga
    37561 taacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc caatgaaggc
    37621 gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt gagaaatcgt
    37681 ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca gtccccagac
    37741 atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag tataagtcag
    37801 cttgtgatta ttttttgttc gttcatgtaa tttgcaccgt ctgatgtacg tcctttttca
    37861 gcgtgaacta ttcacagggt gtatgcttac aggcaagcaa agggagacag actggcctat
    37921 ggatctcaat acactcatct cacaatatgg ttatgccgcg ctggtgatcg gtagcctggc
    37981 ggaaggtgaa accgtgactt tgctgggagg cgttgcggca catcaggggc tattaaagtt
    38041 cccgctggtg gtactttctg tggcgcttgg cggcatgatt ggcgaccagg tgctttatct
    38101 gtgcgggcgg cgctttggcg gcaagctatt acgccgtttc tcgaaacatc aggataaaat
    38161 tgagcgggcg cagaaactta tccaacgcca tccgtatctg tttgtcattg gtacgcgttt
    38221 tatgtatggc tttagggtga ttggcccgac gctgattggt gccagccagc tgccgccgaa
    38281 aatctttctg ccactgaata tccttggcgc atttgcctgg gcgttgattt ttaccactat
    38341 cggttacgct ggtggtcagg tgattgcgcc gtggttgcac aatctcgacc agcatttgaa
    38401 gcactgggtc tggttgattc tggtcgtggt tctggtggta ggcgtgcgct ggtggctgaa
    38461 acggcgcggg aagaaaaagc cggataatca ggcataaagc agttgccaca gataagcatt
    38521 cacgccacat ccggcaccgg atgttccatg cctgatgcga cgctggcgtg tcttatctgg
    38581 cctaacccaa tgaaataacc acgcacctca ctgcgctatt ctgggttgaa ctgcggattc
    38641 gccaacataa agccgccatc cactatcaac gactgcccag tggtgtaatt tgcgccctcc
    38701 gaacaaagcc acaccaccag gctggcaatc tcatgcgttg cgccaaaacg ccgcaaggga
    38761 atcgaaggct ccgcgccggg cttcacgtcg ccatcatcca tgccgttcat tggcgtggcg
    38821 atcgcccaag gtgcaaccgc attcaccaaa atcttatgcc tgaccagctc cagcgccatc
    38881 gctttggtta acccgccgag cgcatgttta gcggctgtgt aggcgctggc atccggcaac
    38941 ggcgtatgtt catgcaccga cgtaatgttg atgatgcgac caccctgccc ttgtttcacc
    39001 atctggcggg cagcaatttg cgagcataaa aatgcaccat cgacatcaac ggtaaaaatc
    39061 ttgcgccact catcgaaagc catatcaaga aacggcgctt tggtcattgc gcccgcatta
    39121 ttcaccagca catcaatacg ccccagccgt tgaatgagtt tctccagcgc cagtgcccct
    39181 tctggtagat tgccgagatc cagctgcacg atctccgcac gtacgccgtg gctaaccacc
    39241 tcacgcgcgg tatcttttgc cccttcttca tcagagtgcc aggtaatacc aatatcaaac
    39301 ccctgctgcg ccagtaataa cgcgcactct ttgccgatcc ccgaatcgga ggcggtaata
    39361 atcgcaacct gtgccatcga gttctccact taacgctgaa taaacgttaa gtatagaagg
    39421 cgcatatcat cagcgtttgt aacccccgcc caacgcacca gtgagttgaa tggaggcatc
    39481 cagccactgc ccttgcaata acaggccatt ggcccgctcg cgaagcgccg ggattctggc
    39541 ttcgctgacg cgggaaccgg caatgatgcc cgcgttaaag cgcgcctgcg caagacctac
    39601 tacacgcaag gcatcgcgct caatttgcgc ctgatgctgg tttttctccg ccagtgtctg
    39661 aacctgactg gctgcccgcg ccacgtcatt caccgcttca accaccgctt tgttgtagct
    39721 ggcgatagac aagttgcttt cggcttttgc gatatcgagg ttggcgttaa gacgaccact
    39781 atcgaaaatg ggtagcgtca ggcctgccgt aacgcccatt tgctgcgcgg aatgacggaa
    39841 cagatcgctt aagtgcaacg catcctgttg caggaaggcc atcaggttga tgtcaggata
    39901 aaatgccgct tttgccgcat caatggtgct tagcgatgac tcaacgtacc agtgcgccgc
    39961 ctgcaaatct gcccgccggg ccagtaagga gtaccccagt tcatcaggaa gctggcttgc
    40021 cactttcggc aacgcgaccg gtttaagctt cagtgacttt gtctggtgat ttgtgagtgc
    40081 atttaaccgt gcctcaataa ttttcatttt ccccgcgaca tcgttgagct gctgccgggt
    40141 tttgctggca ttaatatcgg tttccacacc ttcaactgaa gaagtaatcc cgttctgata
    40201 tagctggcga tcggtggcga taatggtgtt ctgctctttt tcgatttgct gtaagacagt
    40261 gtttaacgcc gcctgggttt gccactccca gtacagacgg gctacgctgc cagccagcaa
    40321 ttggcgggtt tgctcgcgtt ccgccgcccg tgctttaacc gtacccaggc gagcagtaac
    40381 ctccgcccga ttctttcccc agatatcgag atgccagccc gccgttaagc caaaagtacc
    40441 gttggtgtac cacggaccgg tcgtacctgc ggcaggatcg ttcagagcaa acggccccat
    40501 taacccttct gccgacattt tttgccgctc catatccgca gaaaagtcga tctgcggacc
    40561 atcctgagtg gcaactgcct tcgcctgggc ttcagccagc tgaatgcgct gttcagccac
    40621 ctgcatatcc ggtgcgttct gtagtgcatt gttaattaat gaagtgagtt gattatcgtg
    40681 atactccagc caccattggc tgtctggcca accatttttc agcgccgtgg gtaatgcggt
    40741 gtcaacttgt gaagcgggcg tttgctggct taacgcctgg cgggtttcat gcataggcgc
    40801 acacccggcc agcatcagta acagcggaaa acaggcgatg gcgggataaa aggaatcacg
    40861 attcatgggg gaataatcag gtaagaaaag gtgcgcggag attaccgtgt gttgcgatat
    40921 attttttagt ttcgcgtggc aatacatcag tagcaataaa acgacatatc cagaaaaata
    40981 tacactaagt gaatgatatc ttccgattta tcttaatcgt ttatggataa cggcaaaggg
    41041 cttcgttttt tcctatactt attcagcact cacaaataaa ggaacgccaa tgaaaattat
    41101 actctgggct gtattgatta ttttcctgat tgggctactg gtggtgactg gcgtatttaa
    41161 gatgatattt taaaattaat taatgtcatc agatccgaaa ataacgagaa tatttcagtc
    41221 tctcatcctg ttgcgctcct gtcatgtgca ttgcttcata taatcactgg cgcaaggagc
    41281 gcgcaggggg cgaccaatcg ccgccgcccc ctgcaccccc gggctctggc gaacaaaatc
    41341 gccgctgcgc ggtgccctcg gcttatccct tacggctacc gggtcgggcg cgaggtaaca
    41401 tccctgtaaa acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg
    41461 cctcggcgat tttgacgcca ccaaacaacc gtgcggccta ttgataaaga gctaacacat
    41521 tgtcaaaaaa catcactatg gttttttaga gtttctcgat atcaattgcc tgaatagccc
    41581 ttgcaatatc aggggaatta ttcaacaccc gaacatgctg aaataattcc gttgcttcat
    41641 cgtattcttt acgcaaataa ctcaaccact gtttaatccg cgcaacgtga tataacccgg
    41701 tatcgccctg cttttccaga cgggtatatt tttgcagcaa agcaactacc tccggccacg
    41761 gcattcgcgg ttcgttatat tttaccaccc ggctcaggtt gggaatattg agcgccccgc
    41821 gaccaatcat cactgcgtca cagccgctga tcgccatgca ttgttgcgcg ctctgccagt
    41881 cccagatttc accgttggca atcaccggaa tattcagccg ctggcgaatc tcgccaatcg
    41941 cctgccagtc aatatgctcc gcgcggtaac cctgctcttt cgtccgccca tgcaccacca
    42001 gctctgtagc accagcctgt tgaaccgcat cggcgatttc aaatttcttc tcaccgctgt
    42061 cccagcccag acgcactttt acgctgacgg gcaaatgcgc cggtacaact tcacgcatcg
    42121 cttttgcccc ctggtagatg agttcaggat ctttgagtaa cgtcgcgccg ccgccgctac
    42181 cgttaaccgt tttcgacggg cagccgcaat tgagatccac accccaggaa cctaactcga
    42241 ccgcacgggc ggcattctct gccagccatt gtgggaactg acctaacaac tgcacgcgca
    42301 ccagcgtacc ggaaggtgtc cggctggcat tttgtagctc agggcaaatg cgatgaaaga
    42361 cttttattgg cagcagttga tccaccacgc ggacaaactc ggtgatgcac agatcgtagt
    42421 cgttaacttc ggtcagcaat tcacgcacca gagagtcgag tacaccctcc atcggagcca
    42481 gtaacacacg catatcatca cccgcaaaaa aatgaggcgc tatgttagcg cctatggtca
    42541 acggattaaa ggtctgcaaa gagggattgc tgcaaactgc attccctctc tatgcttgat
    42601 gtatgcctga gttcgctgct cattgcgtaa tgcccgggag cgaaaaaata agcgctgatt
    42661 tcttaatgtg atcggtagca cgttttacga attaattgta tgatgaatcc atctcatctg
    42721 gggtgttgat tatgagtaag acactgaaca ttatctggca atatttacgc gctttcgtcc
    42781 tgatttatgc ctgcctgtat gcaggcattt tcattgcttc actgctaccg gtaaccattc
    42841 cgggcagcat catcgggatg ctaatcctgt ttgtcctgct ggccttgcaa atacttccgg
    42901 cgaaatgggt caatccgggg tgctacgtac tgattcgcta tatggcgcta ttgtttgtgc
    42961 cgattggcgt aggcgtcatg caatattttg atttgctccg cgcacagttt ggcccggtag
    43021 tggtttcctg tgcagtcagt acgctggtgg tttttctggt ggtgagctgg agttcgcaac
    43081 tggtgcatgg tgaacgtaaa gtcgtaggtc agaaaggatc agaagaatga tggcgaatat
    43141 ctggtggtca ttaccgctga cattgattgt cttttttgcc gcccgcaaac tggcggcacg
    43201 gtataagttt cccttgctta acccgttgct ggtagcaatg gtggtgatca ttcctttttt
    43261 aatgctgacc ggcatctcct acgacagcta ctttaaaggt agcgaagtgc taaacgatct
    43321 gctgcaaccg gcggtcgtcg cgctggccta tcctttatat gagcagctac accagatccg
    43381 cgcgcgctgg aaatcgatca tcaccatctg ttttatcggc agcgtggttg cgatggtgac
    43441 ggggacttcc gtggcattgt tgatgggcgc ttctccggaa atcgccgcgt caatcctgcc
    43501 gaaatcagtc accacgccta ttgcaatggc ggttggcggc agtattggcg gtattccggc
    43561 aatcagcgca gtttgcgtca ttttcgtcgg catcctcggc gctgtgtttg gccatacatt
    43621 gcttaatgcg atgcgtattc gtaccaaagc tgcgcgcggt ctggcgatgg ggacagcctc
    43681 gcacgccctc ggtacggcgc gctgcgccga gctggattat caggaaggtg cgtttagttc
    43741 gctggcactg gtgttatgcg ggattattac ttcgctgatc gcaccgttcc ttttcccgat
    43801 tattctggca gtaatgggct aaaatttgcg atgcgtcgcg catttttgat gtaagtttca
    43861 cgcgttgcat aattaatgag attcagatca catataaagc cataacgggt tcgtaaactg
    43921 ttatcccatt acatgattat gaggcaacgc catgcatcca cgttttcaaa ccgcttttgc
    43981 ccaacttgcg gataacttgc aatctgcact ggaacctatt ctggcagaca agtacttccc
    44041 cgctttgttg accggggagc aagtctcatc gctaaagagc gcaacggggc tggacgaaga
    44101 cgcgctggca ttcgcactac ttccgctggc ggcggcctgt gcgcgtacgc cattgtcgaa
    44161 ttttaatgtt ggcgcaattg cgcgcggtgt gagcggaacc tggtattttg gtgccaatat
    44221 ggaatttatt ggtgcgacaa tgcagcaaac cgttcatgcc gaacaaagcg cgatcagcca
    44281 cgcctggttg agtggtgaaa aagcgcttgc agccatcacc gttaactaca cgccttgtgg
    44341 tcactgccgt cagtttatga atgaactaaa cagcggtctg gatctgcgta ttcatctgcc
    44401 gggccgcgag gcacacgcgc tgcgtgacta tctgccggat gcctttgggc cgaaagatct
    44461 ggagattaaa acgctgctga tggacgagca ggatcacggc tatgcgctga cgggtgatgc
    44521 gctttctcag gcggcgattg cagcggcaaa ccgttcgcac atgccttaca gtaagtcgcc
    44581 aagcggtgtc gcgctggaat gtaaagacgg tcgtattttc agcggcagct acgctgaaaa
    44641 cgccgcattc aacccgactc tgccaccgtt gcagggggcg ttaattctgt tgaatctcaa
    44701 gggttatgat tacccggata tccagcgcgc ggttctggca gaaaaagccg atgcgccgtt
    44761 gattcagtgg gatgccacct ccgcaacgct gaaagctctc ggctgtcaca atatcgaccg
    44821 cgtgcttctc gcttaagcct ggtgccggat gcggcgtgaa cgccttgtcc ggcttgccag
    44881 ccctctcctg gtgtcgaaaa tcccggcaaa cagtttgccg tttcttgcgc aaaaccagcg
    44941 ggtaaagtag cctgatggaa attttcctta gatcgagtct cctgcatgtt aaagcgcgtg
    45001 ttcctcagcc tgttagtcct gatcggcttg ctgctgttga ctgtgctcgg cctcgatcgc
    45061 tggatgagct ggaaaaccgc gccttatatc tacgacgaat tgcaggatct cccctaccgc
    45121 caggtcggtg tggtgctcgg cacagcaaaa tattatcgta ctggcgtaat taatcagtat
    45181 tatcgctacc gcattcaagg agcgattaat gcctataaca gcggtaaggt aaattatcta
    45241 ttactgagcg gcgataacgc attgcaaagt tataatgagc cgatgaccat gcgcaaagat
    45301 ttaatcgctg ctggtgtcga cccatcagat attgttctcg attacgcagg ctttcgtacg
    45361 ctggattcca tcgtgcgtac acgcaaagtt ttcgatacta atgatttcat tattatcacc
    45421 caacgtttcc actgtgagcg ggcattattt attgcgctgc atatggggat tcaggctcag
    45481 tgttatgccg taccgtcacc gaaagatatg ctgtcagtac gtattcgcga atttgccgcc
    45541 cgtttcggag cgctggccga cctttatatt tttaaacgtg aaccgcgttt tttagggccg
    45601 ctggtcccta ttccggctat gcaccaggtg ccggaagatg cgcaggggta tcccgccgtc
    45661 acgcccgaac agttacttga attgcaaaag aaacaaggaa agtagttatg gatgtacagc
    45721 agttttttgt cgttgccgtt tttttcctta tcccgatatt ttgtttccgc gaagcatgga
    45781 aaggctggcg cgcaggcgcg attgataaac gggttaaaaa tgcaccggaa ccggtgtatg
    45841 tctggcgagc aaaaaatccc ggactcttct tcgcttatat ggtggcatat atcggcttcg
    45901 gaattttatc tatcggcat
//
LOCUS       DAYPHN010000017        45046 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000017 DAYPHN010000000
VERSION     DAYPHN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.017"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1111)
                     /locus_tag="ACG0BW_000973"
     CDS             complement(<1..1111)
                     /locus_tag="ACG0BW_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7257968.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKHDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDASSDLDSYLNLKFDNLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKVGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPAIDFGIDLGNCNCSNVNFEHTYFDSVKFTNT
                     NCTNANFNSCRFIKCDLTNMNCTGAILDNAVIYGKEKEPEMQYPEADQIIQRITYQKS
                     HGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKAEAESTEEKDISPLAKIILDYIESDKN"
     gene            complement(1342..2316)
                     /gene="yajO"
                     /locus_tag="ACG0BW_000974"
     CDS             complement(1342..2316)
                     /gene="yajO"
                     /locus_tag="ACG0BW_000974"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFK7257969.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     RGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2371..4233)
                     /gene="dxs"
                     /locus_tag="ACG0BW_000975"
     CDS             complement(2371..4233)
                     /gene="dxs"
                     /locus_tag="ACG0BW_000975"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFK7257970.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPVVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELSLDAAGMEAKIKAWLA"
     gene            complement(4258..5157)
                     /gene="ispA"
                     /locus_tag="ACG0BW_000976"
     CDS             complement(4258..5157)
                     /gene="ispA"
                     /locus_tag="ACG0BW_000976"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFK7257971.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(5157..5399)
                     /gene="xseB"
                     /locus_tag="ACG0BW_000977"
     CDS             complement(5157..5399)
                     /gene="xseB"
                     /locus_tag="ACG0BW_000977"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFK7257972.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5605..7053
                     /gene="thiI"
                     /locus_tag="ACG0BW_000978"
     CDS             5605..7053
                     /gene="thiI"
                     /locus_tag="ACG0BW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFK7257973.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(7107..7697)
                     /gene="yajL"
                     /locus_tag="ACG0BW_000979"
     CDS             complement(7107..7697)
                     /gene="yajL"
                     /locus_tag="ACG0BW_000979"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFK7257974.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7660..8571)
                     /gene="panE"
                     /locus_tag="ACG0BW_000980"
     CDS             complement(7660..8571)
                     /gene="panE"
                     /locus_tag="ACG0BW_000980"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFK7257975.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8739..9230
                     /gene="yajQ"
                     /locus_tag="ACG0BW_000981"
     CDS             8739..9230
                     /gene="yajQ"
                     /locus_tag="ACG0BW_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFK7257976.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9358..10722)
                     /gene="yajR"
                     /locus_tag="ACG0BW_000982"
     CDS             complement(9358..10722)
                     /gene="yajR"
                     /locus_tag="ACG0BW_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7257977.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAVGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(10871..11761)
                     /gene="cyoE"
                     /locus_tag="ACG0BW_000983"
     CDS             complement(10871..11761)
                     /gene="cyoE"
                     /locus_tag="ACG0BW_000983"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFK7257978.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGRIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11773..12102)
                     /gene="cyoD"
                     /locus_tag="ACG0BW_000984"
     CDS             complement(11773..12102)
                     /gene="cyoD"
                     /locus_tag="ACG0BW_000984"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFK7257979.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12102..12716)
                     /gene="cyoC"
                     /locus_tag="ACG0BW_000985"
     CDS             complement(12102..12716)
                     /gene="cyoC"
                     /locus_tag="ACG0BW_000985"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFK7257980.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12706..14697)
                     /gene="cyoB"
                     /locus_tag="ACG0BW_000986"
     CDS             complement(12706..14697)
                     /gene="cyoB"
                     /locus_tag="ACG0BW_000986"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFK7257981.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14719..15666)
                     /gene="cyoA"
                     /locus_tag="ACG0BW_000987"
     CDS             complement(14719..15666)
                     /gene="cyoA"
                     /locus_tag="ACG0BW_000987"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFK7257982.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(16126..17601)
                     /gene="ampG"
                     /locus_tag="ACG0BW_000988"
     CDS             complement(16126..17601)
                     /gene="ampG"
                     /locus_tag="ACG0BW_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFK7257983.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQIMLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(17645..18223)
                     /gene="yajG"
                     /locus_tag="ACG0BW_000989"
     CDS             complement(17645..18223)
                     /gene="yajG"
                     /locus_tag="ACG0BW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFK7257984.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTMTLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            18528..18845
                     /gene="bolA"
                     /locus_tag="ACG0BW_000990"
     CDS             18528..18845
                     /gene="bolA"
                     /locus_tag="ACG0BW_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFK7257985.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            19189..20487
                     /gene="tig"
                     /locus_tag="ACG0BW_000991"
     CDS             19189..20487
                     /gene="tig"
                     /locus_tag="ACG0BW_000991"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFK7257986.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20733..21356
                     /gene="clpP"
                     /locus_tag="ACG0BW_000992"
     CDS             20733..21356
                     /gene="clpP"
                     /locus_tag="ACG0BW_000992"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFK7257987.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            21482..22756
                     /gene="clpX"
                     /locus_tag="ACG0BW_000993"
     CDS             21482..22756
                     /gene="clpX"
                     /locus_tag="ACG0BW_000993"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFK7257988.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            22944..25298
                     /gene="lon"
                     /locus_tag="ACG0BW_000994"
     CDS             22944..25298
                     /gene="lon"
                     /locus_tag="ACG0BW_000994"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFK7257989.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            25489..25779
                     /gene="hupB"
                     /locus_tag="ACG0BW_000995"
     CDS             25489..25779
                     /gene="hupB"
                     /locus_tag="ACG0BW_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFK7257990.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25971..27842
                     /gene="ppiD"
                     /locus_tag="ACG0BW_000996"
     CDS             25971..27842
                     /gene="ppiD"
                     /locus_tag="ACG0BW_000996"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFK7257991.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27993..28364
                     /gene="ybaV"
                     /locus_tag="ACG0BW_000997"
     CDS             27993..28364
                     /gene="ybaV"
                     /locus_tag="ACG0BW_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFK7257992.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            28458..28856
                     /gene="fadM"
                     /locus_tag="ACG0BW_000998"
     CDS             28458..28856
                     /gene="fadM"
                     /locus_tag="ACG0BW_000998"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFK7257993.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQAITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(28908..29603)
                     /gene="queC"
                     /locus_tag="ACG0BW_000999"
     CDS             complement(28908..29603)
                     /gene="queC"
                     /locus_tag="ACG0BW_000999"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFK7257994.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(29668..31368)
                     /gene="ybaE"
                     /locus_tag="ACG0BW_001000"
     CDS             complement(29668..31368)
                     /gene="ybaE"
                     /locus_tag="ACG0BW_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFK7257995.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTV
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            31468..32286
                     /gene="cof"
                     /locus_tag="ACG0BW_001001"
     CDS             31468..32286
                     /gene="cof"
                     /locus_tag="ACG0BW_001001"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFK7257996.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGCVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            32439..32897
                     /gene="decR"
                     /locus_tag="ACG0BW_001002"
     CDS             32439..32897
                     /gene="decR"
                     /locus_tag="ACG0BW_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFK7257997.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            32927..34699
                     /gene="mdlA"
                     /locus_tag="ACG0BW_001003"
     CDS             32927..34699
                     /gene="mdlA"
                     /locus_tag="ACG0BW_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFK7257998.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGN
                     IRFHDIPLTQLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            34692..36473
                     /gene="mdlB"
                     /locus_tag="ACG0BW_001004"
     CDS             34692..36473
                     /gene="mdlB"
                     /locus_tag="ACG0BW_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFK7257999.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            36654..36992
                     /gene="glnK"
                     /locus_tag="ACG0BW_001005"
     CDS             36654..36992
                     /gene="glnK"
                     /locus_tag="ACG0BW_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFK7258000.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            37022..38308
                     /gene="amtB"
                     /locus_tag="ACG0BW_001006"
     CDS             37022..38308
                     /gene="amtB"
                     /locus_tag="ACG0BW_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFK7258001.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(38357..39217)
                     /gene="tesB"
                     /locus_tag="ACG0BW_001007"
     CDS             complement(38357..39217)
                     /gene="tesB"
                     /locus_tag="ACG0BW_001007"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFK7258002.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            39435..40007
                     /gene="ybaY"
                     /locus_tag="ACG0BW_001008"
     CDS             39435..40007
                     /gene="ybaY"
                     /locus_tag="ACG0BW_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFK7258003.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(40038..40349)
                     /locus_tag="ACG0BW_001009"
     CDS             complement(40038..40349)
                     /locus_tag="ACG0BW_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFK7258004.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            40512..40608
                     /gene="ffs"
                     /locus_tag="ACG0BW_001010"
     ncRNA           40512..40608
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG0BW_001010"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40728..41081
                     /gene="ybaA"
                     /locus_tag="ACG0BW_001011"
     CDS             40728..41081
                     /gene="ybaA"
                     /locus_tag="ACG0BW_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFK7258005.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(41123..42673)
                     /gene="pdeB"
                     /locus_tag="ACG0BW_001012"
     CDS             complement(41123..42673)
                     /gene="pdeB"
                     /locus_tag="ACG0BW_001012"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFK7258006.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGATCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42837..43307)
                     /gene="ylaC"
                     /locus_tag="ACG0BW_001013"
     CDS             complement(42837..43307)
                     /gene="ylaC"
                     /locus_tag="ACG0BW_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFK7258007.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(43423..43974)
                     /gene="maa"
                     /locus_tag="ACG0BW_001014"
     CDS             complement(43423..43974)
                     /gene="maa"
                     /locus_tag="ACG0BW_001014"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFK7258008.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGIPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(44146..44364)
                     /gene="hha"
                     /locus_tag="ACG0BW_001015"
     CDS             complement(44146..44364)
                     /gene="hha"
                     /locus_tag="ACG0BW_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFK7258009.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(44390..44764)
                     /gene="tomB"
                     /locus_tag="ACG0BW_001016"
     CDS             complement(44390..44764)
                     /gene="tomB"
                     /locus_tag="ACG0BW_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFK7258010.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        11331 a        11071 c        11854 g        10790 t
ORIGIN      
        1 ggtttttatc tgattcaata tagtcaagaa taatttttgc taatgggcta atatcttttt
       61 cttctgttga ttctgcttct gcttttaata tcggattaaa aaggtcggta cggtaaagtt
      121 tcgtgccttt catcctgacg atgtcaggaa gaatagtgtt tgaaagatta gcctcgctaa
      181 agtccacatt ttggcaatca ctttctgata aatctgccca attgaaagtg gttttaacac
      241 aggagcagtt agttagaatc atccctttcg tttcattccc atgtgatttc tgataagtta
      301 tcctttgtat tatttgatct gcttctggat attgcatctc aggttctttt tctttaccat
      361 atatcaccgc attatcaaga attgcgccag tgcaattcat atttgttaga tcgcatttga
      421 taaatctaca actgttaaaa ttcgcatttg tacaattcgt atttgtaaac ttgacgctat
      481 caaaataagt atgttcgaag ttgacatttg agcagttaca gtttcccaga tctataccga
      541 aatctatagc gggatcttta ttgggatctt taccggaaat attgctgtgt gctagatata
      601 gaccgctgaa atcgtaatat ccattgtcat ccttttctat tgggagtgaa taagtcttta
      661 ctacctcgtt aacaatagag atagttccac gtatcatcag attttctccg actttataaa
      721 aaataatgtt atgtttgatg gtgctgttgt atggctctgg caatgaggat gcaattttat
      781 tccagaaaca acaaagcgtg gtctgctttt gtttgggcga gaggttatca aatttcaggt
      841 ttaaatatga gtctaaatct gacgatgcat ctatattaaa attgggatct ggagttaacg
      901 ctgaattata atttagaact gacaatgtat tgatgtattt atgaatcgat ttaaatgcct
      961 tttgtttatc actgtccaga aaaaaatcac gaattttgtt ccatagcctt tcgataagat
     1021 tttgatttaa atcgtgcttc attgagtttc ttaaatctgc aaccggtaca tcagtaaaca
     1081 ggatcggttg tttggtgata gttttcatca tgattgtaat cctcttacgt tgactaaata
     1141 agacagtttt agggagtatt acctggagaa actatcaaaa atcgctcaaa gggatgaaga
     1201 tgatgaatag ctgcaaactc attcacgaag aatttctctt gcagaggtgg attatggtta
     1261 cagaagatga tgggaggtac gttgtcccca tcatcgcaga cgatggggaa ttcagcagat
     1321 aaaatggctg aaaagcgaaa tttacttaaa tcctacgacc ggatgcggtt tatacggcgt
     1381 ttccagttca gcaatctgtt ccggcttcaa ggtgatatcc accgcgttca gcagctcatc
     1441 aagctgttct tcccgcgaag ttccgataat cggtgcggca atgccccgtt tactcaacaa
     1501 ccaggccagc gcaacttgtg ctcgtgttgc acccagttct tcactgacgc ccgttaaccg
     1561 ctctgcgatc tgcgcgtcat tttcatcgct ttctttatag agatttttcc ccacctcatc
     1621 agacaccagt cgtgcggtag tttctcccca cggacgtgtc agacgacccc gcgccagcgg
     1681 gctccatgga atcaccgcta cgccctcctg gtaacacagt ggcagcatct cgcgctcttc
     1741 ttctcgataa atcagattgt agtgatcctg catactgaca aactgcgccc agccgtgctg
     1801 tttttgtagt tccagtgcct gagcaaactg cgaagcgtgc atggatgacg cgccgatata
     1861 acgcgctttc ccggctttta ccacgtcgtt cagggcttcc agcgtctctt cgatcggcgt
     1921 gttgtaatcc cagcgatgaa tttgcaggat atcgacataa tccatgccga gacggcgcag
     1981 gctgtcgtcg atagagcgca atatttgcgc acgggataat ccttccggca aatcaccaac
     2041 gcgatggaac actttggtgg caacgaccac gtcttcacga cgggcgaaat cccgcagtgc
     2101 gcgaccaacg atctcttcgc tgctgccatc ggaatagcta ttggcggtgt caaagaaatt
     2161 tatgccgcct tccagcgcgc gtttaattat gggacgactg ctctcttccg gcagtgtcca
     2221 tgcgtgatta ccacgatctg gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac
     2281 gcgaaggtcg gtttttccta aggggttgta ttgcatgctg ccactcctgc catgaataat
     2341 ttcttaagca tagcaggagt ggagtaggga ttatgccagc caggccttga ttttggcttc
     2401 cataccggcg gcatcgaggc tgagttcggc gcgcatttct tcctgcgttc cttgtggaat
     2461 aaagaagtca ggcaggccaa tgttcagcac gggtactggt ttacgatggg ccatcagcac
     2521 ttcgttcact ccgctgcctg cgccgcccat aatggcgttt tcttctacgg tgaccagcgc
     2581 ttcatggctg gcggccattt ccagaattaa cgcttcatca agcggtttca caaaacgcat
     2641 atcgaccagc gtagcgttca gcgattcagc gactttcgcc gcttctggca tcagcgtacc
     2701 aaagttaagg atcgccagtt tctcgccacg acgcttcaca atgcctttgc caattggcag
     2761 tttttccagc ggcgtcagtt ccacgccaac cgcgttgcca cgcgggtagc gtaccgctga
     2821 cgggccatcg ttatagtgat agccggtata gagcatctgg cgacattcgt tttcatcgct
     2881 cggggtcata atgaccattt ccggtatgca gcgcaggtaa gagagatcaa aagcgccctg
     2941 gtgggtttga ccatcagcac caacaatgcc cgcgcggtcg attgcgaaca ggaccggaag
     3001 cttttgaatt gccacgtcat gcagcacctg atcataggcg cgttgcagga aagtggagta
     3061 gatcgcgaca acgggtttgt agccaccaat cgccagaccc gccgcaaagg tcactgcgtg
     3121 ttgctcggca attgccacgt cgaagtagcg atccgggaat ttacgtgaaa actcgaccat
     3181 gccggaacct tcacgcatcg ccggagtaat cgccatcagc ttgttatctt tcgccgccgt
     3241 ttcgcacaac cagtcgccaa agatttttga atagctcggc aaaccgccgc tacttttcgg
     3301 caaacaaccg ctggagggat caaatttagg cacggcgtgg aaggtgattg ggtctttttc
     3361 tgccggttca taaccacgac cttttttggt catgatatgc aggaactgcg gtcctttcag
     3421 gtcgcgcatg ttcttaagcg tagtgataag ccccagcaca tcgtgaccgt caaccgggcc
     3481 gatgtagtta aagcccagct cttcaaacaa cgtgccaggc actaccatgc ctttaatatg
     3541 ttcttcggta cgtttgagca gctctttaat ggggggcacg ccagagaaaa cttttttccc
     3601 gccttcgcgc agtgaagagt aaagcttacc ggaaagcagt tgcgccagat ggttgttgag
     3661 cgcgccaaca ttttcggaaa tcgacatttc attgtcgttg agaaccacca gcatatcagg
     3721 acggatatcg cccgcgtgat tcatcgcttc aaacgccatg ccagcggtaa tagcgccatc
     3781 gccaatgaca cagacggtac ggcgattttt gccttctttc tcggcagcaa ccgcaatacc
     3841 aattccggca ctgatggagg ttgatgaatg cccgacgctt aatacgtcat attcgctctc
     3901 gccgcgccac gggaacgggt gcaggccgcc tttctgacgg atggtgccga ttttgtcgcg
     3961 gcgtccggtc aaaattttat gcggataagc ctgatgcccc acatcccaaa tcaattggtc
     4021 aaacggggtg ttgtagacat agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga
     4081 ggcgaagtgc ccgctggaac ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca
     4141 gagtttcggt aaactctctt tcggcaacag tcgtaactcc tgggtggagt cgaccagtgc
     4201 cagggtcggg tatttggcaa tatcaaaact catcaggggc ctattaatac ttattgttta
     4261 tttattacgc tggatgatgt agtccgctag cgcttccagt gccgaggtat cgagtgactg
     4321 ttcagccagt tgtttcagcg actgacgggc atcgtcgatc agatcccggg ctttcttccg
     4381 ggcttgctca agacccagaa gtgcagggta ggtactttta ccaagttgct gatcggcacc
     4441 ctggcgtttt cctaacgttg cagtatctcc taccacatcc aggatgtcat cctgaacctg
     4501 gaaggcaagg ccgatgctct ctgcgtactt gtcgagtact ggcagagcac gacgtccttt
     4561 atctccggcg cttaatgcac caaggcgaac ggcggcgcga atcaatgcgc cggttttatg
     4621 acgatgaata cgctcaagcg cgtccagagg tacgtgtttg ccttccgcat ctaaatctaa
     4681 tgcctgacca ccgcacattc cggcaatacc gctggcgctc gccagttctg aaatcatcga
     4741 aattctgtcg cgatccgaca cttccggcat atcggcatcg cttaaaatcg agaacgccag
     4801 cgtttgtaaa gcgtcgccag cgagaatcgc gtttgcttcg ccaaacttca catggcaggt
     4861 tggcaaaccg cgacgcagat cgtcatcatc cattgccggt aaatcatcat gaattaatga
     4921 gtaagcgtgg atacactcta cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc
     4981 gaacatatga ccggtggcat aaaccaggaa aggtcgcagg cgcttaccac ctaataatgc
     5041 gccatactgc atggtttcga ccacgggagt gttctgaaag ggcagtgggg cgataaaacg
     5101 gctcagcgcc tggttggcct gcttaacgca ggcttcgagt tgctgcggaa agtccattac
     5161 tcattgtccg gtgtaaaagg ggttagagag gcgtcttcat tgtcagacag cagaatttgt
     5221 acgcgctgtt cggcttgttg taatttggcc tgcccctgac gtgccagctg cacgccgcgt
     5281 tcgaactcgt tcagcgcctc ttccagcggc aggtcgccac tttccagacg ggttacaatc
     5341 tgttccagct cgctcagcgc cttttcaaag ctggcgggcg cctcattttt cttcggcata
     5401 atgaatgtct gactctcaat atttttcgcc ccgtcatggt aacggactca gggcaaatag
     5461 caaataacgc gcaatggtaa ggtgatgtgc gcagcaaagc gatgttagtg gtatacttcc
     5521 gcgcctggat gcagccgcag gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag
     5581 gcttgccaac gaaccattgc cgccatgaag tttatcatta aattgttccc ggaaatcacc
     5641 atcaaaagcc aatctgtgcg cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac
     5701 gttttaaagc actatgatga gacgctcgct gtcgtccgcc actgggataa catcgaagtt
     5761 cgcgcaaaag atgaaaacca gcgtctggcc attcgcgacg ccctgacccg tattccgggt
     5821 atccaccata ttctcgaagt cgaagacgtg ccgtttaccg acatgcacga tatttttgag
     5881 aaagcgttgg ttcagtatcg cgatcagctg gaagggaaaa ccttctgcgt acgcgtgaag
     5941 cgccgcggca aacatgattt tagctcgatt gatgtggaac gttacgtcgg cggcggttta
     6001 aatcagcata ttgaatccgc gcgcgtgaag ctgactaacc cggatgtcac tgtccatctg
     6061 gaagtggaag acgatcgtct cctgctgatt aaaggtcgct atgaaggtat tggcggtttc
     6121 ccgattggta cccaggaaga tgtgctgtcg ctcatttccg gtggtttcga ctccggcgtt
     6181 tccagctata tgttgatgcg tcgcggctgc cgcgtgcatt actgcttctt taacctcggc
     6241 ggcgcggcgc atgaaattgg cgtgcgtcag gtggcgcatt atctgtggaa ccgctttggc
     6301 agctcccacc gcgtgcgttt tgtcgctatt aatttcgaac ctgtcgtcgg ggaaattctc
     6361 gagaaaatcg acgacggtca gatgggtgtt atcctcaaac gtatgatggt gcgtgccgcg
     6421 tctaaagtgg ctgaacgtta cggtgtacag gcgctggtca ctggcgaagc actgggccag
     6481 gtgtctagcc agacgctgac taacctgcgc ctgattgata acgtctccga cacgctgatc
     6541 ctgcgtccgc tgatttctta cgacaaagag cacatcatca acctggctcg ccagattggc
     6601 actgaagact ttgcccgcac gatgcccgaa tattgcggtg tgatctccaa aagcccgacg
     6661 gtgaaagcgg ttaaatcgaa gattgaagcg gaagaagaga agttcgactt cagcattctc
     6721 gataaagtgg ttgaggaagc gaataacgtt gatatccgcg aaatcgccca gcagaccgag
     6781 caggaagtgg tggaagtgga aaccgtcaat ggcttcggcc cgaacgacgt gatcctcgat
     6841 atccgttcta tcgatgaaca ggaagataag ccgctgaaag tcgaagggat tgatgtggtt
     6901 tctctgccgt tctataaact gagcaccaaa tttggcgatc tcgaccagaa caaaacctgg
     6961 ctgttgtggt gtgagcgcgg ggtgatgagc cgcctgcagg cgctctatct gcgcgagcag
     7021 ggctttaaca atgtgaaggt atatcgcccg taatttgtgg tttttacgtc gcatctggtc
     7081 agatgcgacg tttgccgcat ccgccactac tcgtaataat tataaatccc tgccgccatc
     7141 accagttgtg atgccacttc atgggctttt tcacgcccaa ccagcaggtc gataattttc
     7201 agaccaaagt cgatagctgt acccggcccc tgactggtca gcaattttac ccgtgcatcc
     7261 cagacgacgc gcttgtcctg ccattgttcg gcgggtattt tgtctttcag cgtcgggaag
     7321 ccggtcatat tgccaatcgg gaagatatcg tgcggcacca gcacggtggc tggcgcggcg
     7381 caaatagccg cgacgatacg cccggaacgg tggaactgtt taacggtttc aaccagcaga
     7441 gtgctatcgc gaaaacactc tgcgccttta atgccaccag gcagcacgat cacgtcatat
     7501 tcaccgtcag ccacttcgac cagcggcgca tccgccagca gcttcacgcc gcgcgagcag
     7561 gtaatggcca ggttgccatc gctggcgacg ctggcagtgg tgactttgat tccgccacga
     7621 accaagaggt cgatagtggt gacggcttca gtctcttcac taccaggggc gaggcaaacc
     7681 agtgccgatg cgctcatatt cactctcctt tctttttacc atttcaaaca ggcgggtgtt
     7741 ttccggtacg gcaatcccat gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc
     7801 gatttcggtg tggcgcagcg cacggatatc ctgcaacatc gacgagatat tttccgctgt
     7861 ggcatcaatc acctgcatca cgtaatcacg caaatcttct gctgaagtat gatgcccttc
     7921 gcgttcgatt actgccgcga cttcttcgca tatctgcata atttcttgcg gatgatgacg
     7981 taattcacca ttcgggcaat tccagatggc ggtcagtgga ttaatcacac agttgactgc
     8041 cagcttgcgc cacagctcgg cgcgaatatt gttatgccac gcgacgtcag gtaacacggt
     8101 ttgcaaaata tccgccagat aactgtaatc gccgtcctgt tgccgtgccg gaccaatatg
     8161 cgtgattccg tttgccacat gaataatgac attgccgtcg cggcgggcgg catgggtggt
     8221 ggtgcccatc agtaatggct gctgaatgtt ttgcaactct tcgatggtgc ccatgccgtt
     8281 gtgaattaac agtattggcg tagttacagg cagtgtggac gcgaggcttt tgacggcatc
     8341 ggaaacctgc catgctttca gcgtcaccag gagcagatcg ctggtggcga gaaaatcggg
     8401 atcgttggcg gtcagcgatt cgttaaatat cgaaccatct gtctcaacca gattcacact
     8461 acaataaggt tgtggtacgc gcagccagcc ctgaacctcg tgaccctgtt tacaaagtgc
     8521 tgtaagccat aattgcccta aggcaccgca tcccaatacg gtaattttca ttgttcctcc
     8581 tcacccgcaa ccactccggg tgttcaataa ggctatccct taattgtgca tgctgttgcg
     8641 actatgcaca attaagggat acgtcctggt gcaggactgt tggttattta actttgcagg
     8701 tattatgctt cgcatcaaaa atgaagggag aggaaaagat gccatctttc gatattgtct
     8761 ctgaagttga tcttcaggaa gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt
     8821 cccgttttga cttccgtaat gttgaagcct catttgagct gaacgacgcc agcaaaacca
     8881 tcaaagtgtt gagcgagtcc gatttccagg tcaatcagtt gctggatatt ctgcgtgcca
     8941 agctgctgaa gcgcggcatt gaaggcagtt cgctggatgt accggaaaat atcgttcata
     9001 gcggtaaaac ctggtttgtg gaagcgaaac tgaaacaggg cattgagagt gcgacccaga
     9061 agaaaatcgt caagatgatc aaagacagca aactgaaagt gcaggcgcaa attcagggcg
     9121 atgagatccg cgtaacgggt aaatctcgtg atgatttgca ggctgtcatg gcgatggtac
     9181 gtggtggcga tctcggtcag ccgttccagt tcaaaaactt ccgcgattaa tcgcgacaat
     9241 gtgcgttttg ttcatgccga atgcagcatg aacagctaat tcggcctaca aaggtttata
     9301 actcaggaac tttgtaggcc tggtaagcgt agcgcagcag gcaattcgcc tcgtttttta
     9361 tgcctgacga attgcctgtt caacctcaaa gcgattcgtc actttgctgt cgattttcac
     9421 ataagctgaa tgttcttctt ctgcaatcaa cacttctttg acgccttcag tttccagcaa
     9481 acgcactttt aacgcctcgt ttgcggcaat gtccgccggg atttcaatgc gtaagctgct
     9541 gacatacggc ggttctttca tggtactggc gactgccagc cacactgcgg ccagcattgc
     9601 gccagcgaga aataccccct gaccgtcaaa catgccgtca atccagccgc ccagcgaacc
     9661 gccaatcgcc acgccaagaa actggctggt ggagtaaaca cccatcgccg tacctttgta
     9721 acctgctggc gactctttac tgataagtga aggcagaagg gcttccatca aattaaacgc
     9781 cacaaagaaa agctgcacgc cgaccaccag ttgccagaac tgcgtttgtg cgttccacaa
     9841 cacaatttcc gcaaccacga tcaacccgac gcagaagaca aagacttgct tcattttgcg
     9901 cttaacttca gcgtagataa tgaaaggcac gaccgagcca aaggcgatta acatcgttgc
     9961 caggtagacc ttccagtgtt cagccgccgg aaatcccgca tcagccagtt gccctggcag
    10021 ggcaacaaac gtcgacatca gcaaaatatg cagacacata atgccaaagt tgagtttcag
    10081 cagccgcggt tccgccagca ctttactgaa actgcctttc accattccgg actcacgatt
    10141 aagtacgtga gtgctactgt tgggcacaac ccaaatggtc aacgcaatgc cggttgttgc
    10201 cagaatagcg atcatccaga acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc
    10261 aagcaccatc gcaatggcaa aggtaatgcc aaagctcacg ccgataaacg ccattgcttt
    10321 ggtgcggttt tgttcgcgcg tgagatcgga aagcagcgcc ataacggcgg cggcaatcgc
    10381 accagagcct tgtaacgccc gacccagaat aattccccag atggagtcgg aaagcgcagc
    10441 gataacgcta ccaacagcaa acaccgccag tccgccgaca attaatggtt tgcgaccaat
    10501 gcggtcggaa agcagaccaa acggaatctg aaaaacggcc tgcgtcagac cataaatacc
    10561 aatggcaata ccgattaatg cttcgctggc accttgcaga gccatgccgt atgtggtcag
    10621 aaccggcaga accataaaca tgcccagcat gcgcaacgag aatacggtcc ctaaacccca
    10681 ggtcgcgcgc cgctcacctg gcgtcatttt ataatcgttc attaccacct ctgttttaaa
    10741 ttcgcgacta gtgtaaagcg gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata
    10801 tttcgagagg gatatgtttc taatgctaag aaaaaaggtg ccgtagcacc tttttaactg
    10861 agaggttgta ttaccacaca gcagccagca gcgtatgcga atccggcacc ataaaatcaa
    10921 cggacatcat caccgagagg gcagtgatgg cgatgataga gaagccgaac agcttgcgcg
    10981 cccagattct gtcatcagca actttataac cgcgcagagc catgcctaac caccagacgc
    11041 tcaccgccgc ggcgactacc agatatttat acccagcgta accgccaaga gagagcatca
    11101 gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg attcttcgcc accgaaatgc
    11161 cttttaccac tggcaatacc ggaatgtttg ccgcctggta atccttaaag cggaaaatgg
    11221 cgatggcata ggagtgaggc atctgccaca gactgaagat agcaagcagg atcgctgcgc
    11281 cactatcgaa ctcgccggtt actgcacagt agccgatcac cggcggcgca gcgccggaga
    11341 gcgaaccaat caatgtgccg tagactgagt ggcgtttcat gtacaggcta taaacgccga
    11401 cataaaccac aaagcccatc acccccagcc agcaggccag cggattcgcg ccaaaccaca
    11461 gcagcataaa gccagcaata cccagcaagg tggcgtacac cagcgagaca gcaggagaga
    11521 tcaggccttt aaccagcacc cgattcttcg tcctttccat ctttctgtcg atatccctgt
    11581 cgatgtagtt gttaaacaca caacccgacg ccacaaccag tgacacccca accagcgtgt
    11641 agataaacag gggataatca atgcggccct ttgaggccag caggaatccc ccaatcaccg
    11701 agatcaggtt gccaaagatg atgcctggtt tcgttacttg caggtattgc ttaaacatca
    11761 taaccgccgc tcttagtgca tcatcatgtt gtagttaagg ttccacataa tccagatgga
    11821 gcctacaacc aggatagcga tgattagcac ggtgaagaca aacgccgtca tgttccagcc
    11881 ttcatctgat ttggtattca tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac
    11941 tgccattgcc aggattgttc ccagaattac ggccggagag gcagcccctg tcatcaccat
    12001 ccagaacgga atcaccgtca gaatgatcga caggataaag cctgtcatgt aggtttttac
    12061 gctgccatgg gacgcgccgc tgtgatcggt agaatgactc attacatcgc ccccatcaga
    12121 taaacaacag tgaacacaca gatccaaacc acatccagga agtgccagaa caggctcagg
    12181 cacatgatgc gggtacggtt agtgctggtc aggccgcgac gagcgatttg taccatcagc
    12241 accgccatcc agataagacc ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag
    12301 aacgctgaca ggaagccgct gcgatccgga cccatgccgt taacaatcag gtgatggaat
    12361 tcgtagattt ccatcccgat aaatccggca ccaaacaacc aggtcaacgc cagccaggag
    12421 ataacctggc ttttgttgtt tttgtacatg gcgatagccg ccatgccgta ggtgatggag
    12481 ctgaacaaca gcaagaaagt ttcaaccaga acgaacggca gttcgaaaat gtccttacct
    12541 gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg tagcaaacaa gattgagaac
    12601 agaatgcagt cgctcatcag gtagatccaa aatccgaaga ttttggttcc gcctgcatcg
    12661 tggtgcccgt gttcgtgcgc gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt
    12721 cagccctgcc ttagtaatct catcgaaatg ctggttttcc agtttttcga tttctgccac
    12781 cggcacgtag taatccacgt cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc
    12841 tgcgaagcca acaatcgcca gccaccagat atgccagatc atggcgaaac cgaagatggt
    12901 ggagaaagct gcaatgacga tacctgcacc gctgtttttc ggcatatgaa tttcttcata
    12961 gtggtcaggc tttttgtacg cttcgccttt ctctttcatt tcccagaatg catcacgctc
    13021 gtgaacgtgc ggcacaacgg caaagttata gaacggaggc ggggaagagg ttgcccactc
    13081 cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg ttctggtcgc ggtcacgaat
    13141 agaaacgtac atctgaataa cgaggcagag aatacccagc gcaatcagta ctgcaccgct
    13201 ggctgcaatc atcagcatgg tgtggaactg cgggtcaatc tgctggctca aacgacgggt
    13261 catgcccatg aagcccagcg catacagcgg cataaaggca acgaagaagc cgatgatcca
    13321 gaaccagaac gcgcgtttac cccaggtttc gttcagtttg aaaccgaacg ctttaggcca
    13381 ccagtaggtc atccctgcga agcagccgaa gaccacgccg ccgatgatca cgttatggaa
    13441 gtgcgcaatc aggaacaggc tgttatgcag aacgaagtcc gcgcccggta ctgccagcag
    13501 cacgccagtc atcccgccca ccgagaaggt gacgataaaa ccgatagtcc acagcatcgc
    13561 agaatggaac acgatgcggc cctgatacat ggtgaacagc cagttgaaga tcttcacccc
    13621 ggtcgggatg gcgataatca tggtggtgat accaaagaag gcgtttacgt tcgcgcccgc
    13681 acccatcgta aagaagtggt gcagccagac gatgaacgac agcacggtga tacagacggt
    13741 tgcccatacc agcgaggtat aaccaaacag acgtttacgc gagaaggttg ccgcaatttc
    13801 ggagaacaca ccgaatacag gcaggatcag gatgtaaact tccgggtggc cccaggccca
    13861 aatcaggttg atgtacatca tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc
    13921 cagatagcga tccagggtca acaacgcgac ggtaaccgtc agaattggga aggaagcaat
    13981 aatcagaacg ttcgcgcaca gtgatgccca ggtaaatact ggcatcttga acatggtcat
    14041 gcccggtgcg cgcatcttca gaatggtaac gaagaagttg ataccggtta gcgtcgtacc
    14101 tataccggat agctggagac tccatatcca gtaatcgaca ccgactcccg gactgtactc
    14161 tattcccgat agcggtggat aggccagcca gccggtctgc gcaaattcac ccacgccgag
    14221 agaaacgtta accagaatca caccaacaac ggtaaaccag aagcttaggt tgttgaggaa
    14281 cgggaacgca acgtcacgcg cgccgatctg cagcggaacc accaggttca tcagaccgat
    14341 aacgaaaggc atcgccacga agaagatcat aatcacgccg tgcgcggtga agatctgatc
    14401 gtagtggtga ggtggcagga agcccgcttc gcccgcagag gcaagggcct gctggctacg
    14461 catcataatg gcgtcagcaa aaccacgcag caacatcaca atcgccacga tgatatacat
    14521 gataccgagg cgtttatggt cgacggaggt cagccactct ttccacaggt aggtccactt
    14581 accgaagtaa gtgatcaggc caacgagcgc cagacctccc aaaataatgc cagcgatcgt
    14641 aaccatgacg ataggttcat ggaacgggac tgcatcaagt gataattttc cgaacatctt
    14701 tattcttcct caaccccttt aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat
    14761 accttcatgt gcgctgtgct caccttctgg ctgggtcatg tccatgctct taccgtgagc
    14821 cataaacttg ttaatcacat cagcaaacaa gtctggtttc acgttggaga aatattccac
    14881 ctggttgtac tcgctaggcg cggccagttt ttcgaacgct gccatgtcag acatggtgtt
    14941 cggcgactgt ttcgcttttg cgacccactg gtcgaatgcg gcgcgatccg gtgttgcaat
    15001 agctttgaac ttcatgcctg agaagcccgg gccgctgtag ctggcggaga taccgtcata
    15061 agtaccgggt tcgttggcga tcagatgcag gcgagtctgc ataccggcca tggcataaat
    15121 ctggctaccc agacgcggaa tgaagaagga gttcatcacg gagttggagg tcactttgaa
    15181 gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg gtagcaatgc cctgttccgg
    15241 gtagatgaag aaccatttcc agtccatgga aaccacttcg atggtaatgg gcttctcgtc
    15301 gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag
    15361 gaagatgatg attaagatag gtaccgtcca gaccacagct tccactttat tggagtgtga
    15421 ccagttcggg ctgtacttag catctttatt gctcgcacgg tacttccagg cgaaaccaac
    15481 agccatcaag attgcgggaa taacgacaat caacatcagg ccaaatgccg tcagtatcag
    15541 tgaacgttgc tccagaccaa tctgtccttt gggatctaac agcgcagaat tacagccact
    15601 gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa cttttattgt atttcctgag
    15661 tctcatttaa cgacctcaat tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt
    15721 acgggaaggt tacattactg taaacatgat taaattcctg ttacttggat ttggcttcca
    15781 ggtcacatat gttgcaaaac atatcaggtt gattttaaga agctgttgca aaagggcgtt
    15841 ataacaaaag gggagtgcag aaggagtctg ggcgggcaat tggtataacc aatgcgaaat
    15901 aaaacaatta tttaacaaat aattatcatt aaggtgacaa ctgggtagtg agaaaataaa
    15961 gactcattag ctgaatcgtg taagaaaaat agaattcata aatggcgtca ataatttcca
    16021 gacgctaagc cgcacaaaag aacaaatatt aataagcgat gctgatgttt atttggccgt
    16081 aataattacg gcgagtgatt actacggcta aataatattt acagattacg tcagatgcgt
    16141 ttttcgtagc gccagataat ccagtaaacc accaagcacg acgccagaaa gggcgactaa
    16201 aaccccgact tccagcagag caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat
    16261 cgtcaacagc agtaaccaca cggccaacag gctgacgccc gccgccagtg tccacatggc
    16321 aaaggcataa cctgcaggat attcggtacg ggagataaag ttgtcattta ctcgtgtata
    16381 ttcaagcgtc tggcggcaaa ccagcagcaa aataagccct ggtacggcag cggcgacgga
    16441 gaacagatag aatgtcgacc agccgtgtgc ttcaacaaac caacccgcca cggggccgac
    16501 ataaactcgc cctacagcag aaagcgctga gagcagggca aattgagtag cggaaaatga
    16561 cttattacac agcgtcatta acagcgcgac aaaggctgat gtacccatcc cgccacagag
    16621 gttttcgaaa aagacggctg cgcccatgct gtagagatgc ttatcagtaa tcgacagcag
    16681 ccagtaaccg gcgttagacg caccttgtaa aataccgaaa atcagcagcg cccggaacag
    16741 tgacaggcgc tgcatcaaaa tcccaccgta caatgcgcca acaatggtcg ccagtaagcc
    16801 aagcgttttg ttaaccacgc ctacttcacc cgcatcaaac ccgacgccac gaatcaaaaa
    16861 cgtggttgtc aggctcatgg cgaatgcgtc gcccagctta tacagcacga taagaagcaa
    16921 aataagccag gcattattgc gaccaaagaa atcgcgcaga ggtgcaacaa ccgcttgttc
    16981 cagcgttttg ggcacaggaa tggtgtcggt tggttctggt gcaagtaacg tcgcgataat
    17041 acaggggatc aacagcgccg ccatcaacca gtacatgccc tgccagccca gccatttatc
    17101 tgccagccac agggctaagc cgccggaaac cagcatccct aaacggtaac ccagcacgct
    17161 gattgccgcg cccgcaccac gttcttctgc cgggagcaca tcggttttcc acgcatcgaa
    17221 gacaatatcc tgggaggcag agcaaaaagc gatcaccact gccagtgccg ccatccagcg
    17281 gagctgggtg ccgggttcga gaaaacccat cgccgcaatg gcgactaata acatgatttg
    17341 cgtggcgagc agccaaccgc gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag
    17401 cggtgaccag aggaatttaa aaacgtaagc ctggcctacc agcgagaaga aaccaatggt
    17461 tttgaggtcg atattctcga ccgtcatcca ggcctgtaag gtgccggatg tcagggcgag
    17521 cggtagcccg gaagcaaaac ccaggatcag caatatggct gaacgcggct gttgaaaaat
    17581 acgtaaatat tgactggaca tgggcgtcta caggcccggc ttgcaccggg ccagagggca
    17641 gaaattaacg cgcgttctgc ttgatgaatt cgtggatgct ggtgtcctga gacatatcag
    17701 cgatggtatc ggtcagcaca ctgttaaccg catcggcgat atttttgttg gaggcctgga
    17761 acgcaccttc aacgttgtag ctggcacgat agttcttggt cattttgttg ccattctgcg
    17821 cggtagcgat gatggcaata tccgctttag tagcgatgtt gtagcgcacg ttgccctggg
    17881 acacgtcagc atacagttgg ctaacgatga tttgcagatt aaccgggcca ttcggaccaa
    17941 ccatgtaacc acgcgcggtc atctgttttt ccagcacttc ttgcagcagg aaacgcagat
    18001 cgcgggaggc ggtcagggta acgatttgat tatcgcgggt gacttttgcc agcgcctgat
    18061 cggtacgctg atcggcacca ttaatgctta cggtgacgcc catcaggctt ggatcctgct
    18121 gtggcagtgt catcgtcggg gaaacttcaa tagttgttgg cggttttgcg catcctgcaa
    18181 gcataaacag agcaactaac gggaagagga tttttttgaa catgttcggg ctctcagaga
    18241 ctcttaagcg tgtttggtaa aaattcccgc catcataaca ttgccaacgg cgaggggaag
    18301 tgggtaaggc atgtaaattc atcatgttga cgaaataatc gcccctggta aaagaaacgc
    18361 tgatgcgagg cctgtgtttc aatctttaaa tcagtaaact tcatacgctt gacagaaaaa
    18421 ccaggacgaa acctaaatat ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt
    18481 atttaaaggg atggatgaca tctcagcgtt gtcggaggag atatttcatg atgatacgtg
    18541 agcggataga agaaaaatta agggcggcgt tccaacccgt attcctcgaa gtagtggatg
    18601 aaagctatcg tcacaatgtc ccagccggct ctgaaagcca ttttaaagtt gtgctggtca
    18661 gcgatcgttt tacgggtgaa cgttttctga atcgtcatcg aatgatttac agtactttag
    18721 cggaggaact ctctactacc gttcatgcgc tggctctgca tacttacact attaaggagt
    18781 gggaagggtt gcaggacacc gtctttgcct ctcctccctg tcgtggagca ggaagcatcg
    18841 cgtaaaaacg catttgcaac tgtcggcgct tttccagtat gttgctaaag attttatgaa
    18901 aaacggcctg cgggccgttt tgttttgtct ggattttgcg ctttttgccc agcattcaga
    18961 cgaaaattgc ccgggaattg tgaaaaaata cgcgacagcg cgcaataacc gttctcgact
    19021 cataaaagtg atgccgctat aatgccgcgt cctatttgaa tgctttcggg atgattctgg
    19081 taacagggaa tgtgattgat tataagaaca tcccggttcc gcgaagccaa caacctgtgc
    19141 ttgcggggta agagttgacc gagcactgtg attttttgag gtaacaagat gcaagtttca
    19201 gttgaaacca ctcaaggcct tggccgccgt gtaacgatta ctatcgctgc tgacagcatc
    19261 gagaccgctg ttaaaagcga gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc
    19321 cgcaaaggca aagtgccaat gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag
    19381 gacgttctgg gtgacctgat gagccgtaac ttcattgacg ccatcattaa agaaaaaatc
    19441 aatccggctg gcgcaccgac ttatgttccg ggcgaataca agctgggtga agacttcacc
    19501 tactctgtag agtttgaagt ttatccggaa gttgaactgc agggtctgga agcgatcgaa
    19561 gttgaaaaac cgatcgttga agtgaccgac gctgacgttg acggcatgct ggatactctg
    19621 cgtaaacagc aggcgacctg gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta
    19681 accatcgact tcaccggttc tgtagacggc gaagagtttg aaggcggtaa agcgtctgat
    19741 ttcgtactgg cgatgggcca gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc
    19801 cacaaagctg gcgaagagtt caccatcgac gtgaccttcc cggaagaata ccacgcagaa
    19861 aacctgaaag gtaaagcagc gaaattcgct atcaacctga agaaagttga agagcgtgaa
    19921 ctgccggaac tgactgcaga attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa
    19981 ggtctgcgcg ctgaagtgcg taaaaacatg gagcgcgagc tgaagagcgc catccgtaac
    20041 cgcgttaagt ctcaggcgat cgaaggtctg gtaaaagcta acgacatcga cgtaccggct
    20101 gcgctgatcg acagtgaaat cgacgttctg cgtcgtcagg ctgcacagcg tttcggtggc
    20161 aacgaaaaac aagctctgga actgccgcgc gaactgttcg aagaacaggc taaacgtcgc
    20221 gtagttgttg gcctgctgct gggcgaagtt atccgcacca acgagctgaa agctgacgaa
    20281 gagcgcgtga aaggcctgat cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt
    20341 atcgagttct acagcaaaaa caaagaactg atggacaaca tgcgcaatgt tgctctggaa
    20401 gaacaggctg ttgaggctgt actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc
    20461 aacgagctga tgaaccagca ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa
    20521 ggcccgtcac cgccaggtgg tgggcttttt tttgtcatga attttgcatg gaaccgtgcg
    20581 aaaagcctct ttcggtgtta gcgtaacaac aaaagattgt tatgcttgaa atatggtgat
    20641 gccgtaccca taacacaggg actagctgat aatccgtcca taaggttaca atcggtacag
    20701 caggtttttt caattttatc caggagacgg aaatgtcata cagcggcgaa cgagataact
    20761 ttgcacccca tatggcgctg gtgccgatgg tcattgaaca gacctcacgt ggtgagcgct
    20821 cttttgatat ctattctcgt ctacttaagg aacgcgtcat ttttctgact ggccaggttg
    20881 aagaccacat ggctaacctg attgtggcgc agatgctgtt cctggaagca gaaaacccag
    20941 aaaaagatat ctatctgtac attaactctc caggcggggt gattactgcc gggatgtcta
    21001 tctatgacac catgcagttt atcaagcctg atgtcagcac tatctgtatg ggccaggcgg
    21061 cctcgatggg cgctttcttg ctgactgcag gggcaaaggg taaacgtttc tgcctgccga
    21121 attcgcgcgt gatgattcac cagccgttgg gcggctacca gggccaggcg accgatatcg
    21181 aaattcatgc ccgtgaaatt ctgaaagtta aagggcgcat gaatgaactt atggcgcttc
    21241 atacgggtca atcattagaa cagattgaac gtgataccga gcgcgatcgc ttcctttccg
    21301 cccctgaagc ggtggaatac ggtctggtcg attcgattct gacccatcgt aattgatgcc
    21361 agaggcgcaa ctgtgccgct atacttatcc agggcggcac aacgctgtaa gcggcttgcg
    21421 cctgagaatg gcatttgcgt cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc
    21481 catgacagat aaacgcaaag atggctcagg caaattgctg tattgctctt tttgcggcaa
    21541 aagccagcat gaagtgcgca agctgattgc cggtccatcc gtgtatatct gcgacgaatg
    21601 tgttgattta tgtaacgaca tcattcgcga agagattaaa gaagttgcac cgcatcgtga
    21661 acgcagtgcg ctaccgacgc cgcatgaaat tcgtaaccac ctggacgatt acgttatcgg
    21721 ccaggaacag gcgaaaaaag tgctggcggt cgcggtatac aaccactaca aacgtctgcg
    21781 caacggcgat accagcaatg gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc
    21841 gaccggttcc ggtaaaacgc tgctggctga aacgctggcg cgcctgctgg acgtcccgtt
    21901 caccatggcc gacgcaacca cgctgaccga agccggttat gtgggcgaag acgttgaaaa
    21961 catcattcag aagctgttgc aaaagtgcga ttacgacgta cagaaagcgc agcgcgggat
    22021 tgtctacatc gatgaaatcg acaagatttc tcgtaagtca gacaacccgt ccattacccg
    22081 agacgtttcc ggtgaaggcg tacagcaggc actgttgaaa ctgattgaag gtacggttgc
    22141 cgccgttcca ccgcagggcg ggcgtaaaca cccgcagcaa gagttcttgc aggtagatac
    22201 ctctaagatc ctgtttattt gtggtggtgc gttcgctggt ctggataaag tcatttccca
    22261 tcgtgtagaa accggctccg gcattggttt tggcgcgacg gtaaaagcga agtccgacaa
    22321 agcaagcgaa ggcgagctgc tggcgcaggt tgaaccggaa gatctgatca agtttggtct
    22381 gatccctgag ttcattggtc gtctgccggt tgtagcaacg ttgaatgaac tgagcgaaga
    22441 agctctgatt cagatcctca aagagccgaa aaacgccctg accaagcagt atcaggcgct
    22501 gtttaatctg gaaggcgtgg atctggaatt ccgtgacgag gcgctggatg ctatcgctaa
    22561 gaaagcgatg gcgcgtaaaa ccggtgcccg tggcctgcgt tccatcgtag aagccgcact
    22621 gctcgatacc atgtacgatc tgccgtccat ggaagacgtc gaaaaagtgg ttatcgacga
    22681 atcggtaatt gatggtcaaa gcaagccgtt gctgatttat ggcaagccgg aagcgcaaca
    22741 ggcatctggt gaataattaa ccattcccat acaattagtt aaccaaaaag gggggatttt
    22801 atctcccctt taatttttcc tctattctcg gcgttgaatg tgggggaaac atccccatat
    22861 actgacgtac atgttaatag atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg
    22921 aaattaaact aagagagagc tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg
    22981 tattgccgct gcgcgatgtg gtggtttatc cgcacatggt catcccctta tttgtcgggc
    23041 gggaaaaatc tatccgttgt ctggaagcgg cgatggacca tgataaaaaa attatgctgg
    23101 tcgcgcagaa agaagcttca acggatgagc cgggtgtaaa cgatcttttc accgtcggga
    23161 ccgtggcctc tatattgcag atgctgaaac tgccagacgg caccgtcaaa gtgctggtcg
    23221 aggggttaca gcgcgcgcgt atttctgcgc tctctgacaa tggcgaacac ttttctgcga
    23281 aggcggagta tctggagtcg ccgaccattg atgagcggga acaggaagtg ctggtgcgta
    23341 ctgcaatcag ccagttcgaa ggctacatca agctgaacaa aaaaatccca ccagaagtgc
    23401 tgacgtcgct gaatagcatc gacgacccgg cgcgtctggc ggataccatt gctgcacata
    23461 tgccgctgaa actggctgac aaacagtccg ttctggagat gtccgacgtt aacgaacgtc
    23521 tggaatatct gatggcaatg atggaatcgg aaatcgatct gctgcaggtt gagaaacgca
    23581 ttcgcaaccg cgttaaaaag cagatggaga aatcccagcg tgagtactat ctgaacgagc
    23641 aaatgaaagc tattcagaaa gaactcggtg aaatggacga cgcgccggac gaaaacgaag
    23701 ccctgaagcg caaaattgac gcggcgaaaa tgccgaaaga ggcaaaagag aaagcggaag
    23761 cagagttgca gaagctgaaa atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg
    23821 gttatatcga ctggatggta caggtgccgt ggaatgcgcg cagcaaggtc aaaaaagacc
    23881 tgcgtcaggc gcaggaaatc cttgataccg accattatgg tctggagcgc gtgaaagatc
    23941 gcatccttga gtatcttgcg gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct
    24001 gcctggtagg gccgccgggg gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca
    24061 ccgggcgtaa atatgtccgt atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg
    24121 gtcaccgccg tacttacatc ggttctatgc cgggtaaact gatccagaaa atggcgaaag
    24181 tgggcgtgaa aaacccgctg ttcctgctcg atgagatcga caaaatgtct tctgacatgc
    24241 gtggcgatcc ggcctccgca ctgcttgaag tgctggatcc agagcagaac gtagcgttca
    24301 gcgaccacta cctggaagtg gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga
    24361 actccatgaa cattccggca ccgctgctgg atcgtatgga agtgattcgc ctctccggtt
    24421 ataccgaaga tgaaaaactg aacatcgcca aacgtcacct gttgccgaag cagattgaac
    24481 gtaatgcact gaaaaaaggt gagctgaccg tcgacgatag cgccattatc ggcattattc
    24541 gttattacac ccgtgaggcg ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgtc
    24601 gcaaagcggt taagcagtta ctgctcgata agtcattaaa acatatcgaa attaacggtg
    24661 ataacctgca tgactacctc ggtgttcagc gtttcgacta tggacgcgct gataacgaaa
    24721 accgtgtcgg tcaggtaact ggtctggcgt ggacggaagt gggcggtgac ttgctgacca
    24781 ttgaaaccgc gtgcgttccg ggtaaaggca aactgaccta taccggatcg cttggcgaag
    24841 tgatgcagga gtccattcag gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg
    24901 ggatcaaccc tgatttttac gaaaaacgcg acatccacgt ccacgtaccg gaaggtgcga
    24961 cgccgaaaga tggtccgagt gccggtattg ctatgtgcac cgcgctggtt tcttgcctga
    25021 ccggtaaccc ggttcgtgcc gatgtggcaa tgaccggtga gatcactctg cgtggtcagg
    25081 tactgccgat cggtggtttg aaagaaaaac tactggcagc gcatcgtggc gggattaaaa
    25141 cagtgctaat tccgttcgaa aataaacgcg atctggaaga gattcctgac aacgtaattg
    25201 ccgatctgga cattcatcct gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa
    25261 atgaaccgtc tggcatgcag gttgtgactg caaaatagtg acctcgcgca aaatgcacta
    25321 ataaaaacag ggctggcagg ctaattcggg cttgccagcc tttttttgtc tcgctaagtt
    25381 agatggcgga tcgggcttgc ccttattaag gggtgttgta aggggatggc tggcctgata
    25441 taactgctgc gcgttcgtac cttgaaggat tcaagtgcga tataaattat aaagaggaag
    25501 agaagagtga ataaatctca attgatcgac aagattgctg caggggctga tatctctaaa
    25561 gctgcggctg gccgtgcgtt agatgctatt attgcttccg taactgaatc tctgaaagaa
    25621 ggggatgatg tagcactggt aggttttggt acttttgccg ttaaagagcg tgctgcccgt
    25681 actggccgca acccgcagac cggtaaagag atcaccatcg ctgccgctaa agtaccgagc
    25741 ttccgtgcag gtaaagcact gaaagacgcg gtaaactaag cgttgtcccc agtggggatg
    25801 tgacgaagtt caagggcgca tctactgatg tgcctttttt atttgtattc ggtgactttc
    25861 tgcgtcttgt gggctgacaa ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc
    25921 gatacgttgc gtgaggtaca cagtcatcta cagcggagtg ttgttacacc atgatggaca
    25981 gcttacgcac ggctgcaaac agtctcgtgc tcaagattat tttcggtatc attatcgtgt
    26041 cgttcatatt gaccggcgtg agtggttacc tgattggcgg aggcaataac tacgccgcaa
    26101 aagtgaatga ccaggaaatc agccgtgggc agttcgagaa tgccttcaac agcgagcgta
    26161 atcgcatgca gcaacagctg ggcgatcaat actccgagct ggcagcgaac gaaggctata
    26221 tgaaaaccct gcgtcaacag gtgctgaatc gtctgatcga cgaggcgctg ctggatcagt
    26281 acgctcgtga gctgaaactg ggtatcagcg atgagcaggt taaacaggcg atttttgcga
    26341 ccccagcctt ccaggttgac ggcaaatttg ataacagccg ctataacggt atcctcaacc
    26401 agatggggat gaccgccgat cagtacgccc aggcgctgcg taaccagctc actacccaac
    26461 agctgattaa cggcgttgcc ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg
    26521 cggcactggt agctcaacaa cgcgtggtgc gtgaagcgac tatcgatgtt aacgcgctgg
    26581 cggcgaagca gcctgtgacc gaacaggaaa ttgccagcta ctacgaacaa aacaaaaaca
    26641 atttcatgac gccggaacaa ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc
    26701 agcaaccggt tagcgatgcg gatatccaga gctactacga tcagcatcag gatcaattca
    26761 cccagccgca gcgtacccgc tacagcatca tccagaccaa aactgaagat gaagcgaaag
    26821 cggtacttga tgagctgaat aaaggcggtg attttgctgc attagccaaa gaaaaatctg
    26881 ccgatattat ctctgctcgt aacggcggcg atatgggttg gttagaagat gccactatcc
    26941 cggacgaact gaaaaatgct ggtctgaaag aaaaaggcca actgtctggt gtcatcaaat
    27001 cttcggtcgg tttcctgatt gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt
    27061 tagacgaagt acgtgacgac attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt
    27121 actatgcgct gcagcagaaa gtgagcgatg cggcaagcaa cgacaccgag tctctggccg
    27181 gtgcagagca agcagccggc gttaaagcca ctcagacggg ttggttcagc aaagataacc
    27241 tgccggaaga gttgaacttc aagccagttg ccgacgctat cttcaacggc ggtctggtag
    27301 gtgaaaacgg cgcgccgggc atcaactctg acatcatcac cgtagacggc gaccgcgcat
    27361 tcgtgctgcg catcagcgag cacaaaccgg aagcggtgaa accgttggca gatgttcagg
    27421 aacaagttaa ggcactggtt cagcacaaca aagctgaaca acaggcgaaa gtggatgctg
    27481 agaaactgct ggttgatttg aaagccggca aaggtgcgga agctatgcag gctgccggtc
    27541 tgaaatttgg cgagccgaaa accttaagcc gttccggtcg tgacccgatt agccaggcgg
    27601 cgtttgcact accactgcca gcgaaagaca aaccgagcta cggtatggcg accgatatgc
    27661 aaggtaatgt ggttctgctg gcgctggatg aagtgaaaca aggttcaatg ccggaagatc
    27721 agaaaaaagc gatggtgcag ggtatcaccc agaacaacgc acaaatcgtc tttgaagcgc
    27781 tgatgagtaa cctgcgtaaa gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat
    27841 aatcctgaag gcgcctcgca aaaaaatgct ttgcagctgt aacaagaaaa ggtcgctttc
    27901 gcggcctttt ccatttctga acattgccat ttgtttcctg ttttcactgc cgttaaggtg
    27961 tttccactgt taacaaacaa ggagaaaaca gtatgaaaca cggaattaaa gcactgctca
    28021 ttaccctgtc tctggcttgt gccggaatgt ctcatagcgc gctggcggca gctcctgcgg
    28081 cgaaaccgac aacggtagaa accaaagcgg aagctcctgc agcacaaagt aaagcagcag
    28141 taccggcgaa agccagtgac gaagaaggct cccgggtcag cattaataat gccagcgcgg
    28201 aagagttagc ccgcgcgatg aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt
    28261 atcgcgaaga gtacggtccg tttaaaacgg tggaggatct aaagcaggtg ccggggatgg
    28321 gcaattcgct ggtggaacgt aatctggcgg tattaaccct gtaattaatt tgcatagtgg
    28381 caattttttg ccagactgaa gaggtcatac cagttatggc ctctgtactt ataacaacaa
    28441 cgtaaggtta ttgcgctatg caaacacaaa tcaaagttcg tggatatcat ctcgatgttt
    28501 accagcacgt taacaacgct cgctaccttg agtttctcga agaagcccgc tgggatgggt
    28561 tggaaaatag cgacagtttt cagtggatga cggcccataa catcgccttc gtcgtggtca
    28621 atatcaatat taactatcgt cgcccggcgg tattaagcga cctgttaacc attaccagtc
    28681 agttgcagca attaaacggt aaaagcggca tcttaagcca ggccattaca ctggagccgg
    28741 aagggcaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt aaaacgcaga
    28801 aagcattagc tctggaaggg gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt
    28861 tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta cttcaacccg
    28921 gttttctgct tcatcgctgc catcaccgtc ggtttatcgg ccagataatg attcaaaccg
    28981 ttggcgcgta aattacacgc cgcacaatga ccgcaaccgt cgcctttaat gccgttatag
    29041 caggtcaacg tttcgttacg gactaaatct agtttgccgt aataatctgc cagcgcccag
    29101 gtttccgctt tatcaatcca catcagcgga gtttcaaaac gaatatcttt cgccatgcct
    29161 aaactgacgg catggtttag tgctttcaca aactcatcgc ggcaatccgg gtaaccggag
    29221 aaatccgttt cgcagacgcc ggtaattacg gcttctgctt ttacctgata cgcatatatt
    29281 gccgccagtg tcaggaacaa aatattacgc cctgggacaa acgtattcgg gatgccatca
    29341 gcttcaggtt cataatcagg caccggaatg ctgtcacgtg tcaggctgct gaccgccagc
    29401 tcgttaagca gcgttacatc aagaacctta tgcgcgcgtg cccccagttt cagcgccagt
    29461 tcgcgtgcca cgtcgatttc tgcgcgatgc cgctgaccgt aatcgaacgt cacgcaatgg
    29521 acttcatcat attgttgtaa tgcctgcacc agacaggtgg tggaatcctg gcctccactg
    29581 aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc gttatggtaa
    29641 cgcctgcgat taacccggac cagctattca ttgcgacggc gcgggaagcc aggcttcggt
    29701 aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag gggcactaat
    29761 gcgatagtga tagttgaata gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac
    29821 cgcttttagg gcattaaatc ggttttcctc atcaggcatt acttgcaccg cgtccagtgt
    29881 cgattgcaaa tgtgcatatg ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg
    29941 cagccattgc tccagggtat attccggtgc ttcgccaatt aatctgtcgc ccatcatgag
    30001 gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt
    30061 gagttcacag ccttccgctg ccagtgtcgc ctgtagacgt tctgccatgg tatgaagttc
    30121 tatcggtagg tgataaacca gtgtcaaggt tttcggtagt ttgacctcat ccggtacttg
    30181 ccattgcgga atagtccagc ctggcagtaa tgcatgactg gcggtgatca ggttttctcc
    30241 gacttctaac gtttgtaata aaccggattg atgaataatg gagatcactt ttcgcgcctg
    30301 ccagagggac agtcggggac ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc
    30361 actactgacc tggctgaccc gttgcaactc ctccggtttg ccgatagtga tttgcacggg
    30421 atgccgacaa ctggttccca aatctttttc gaaaagcggc ggagttatcc agtactcaac
    30481 cgctttaagc agcggatgac gtaaatggta ataatcatga ctctccaggc gcaccagttc
    30541 cactgtgaat tgtgttaagc gaaaaggacc agtgccgatc agggggaatt gcggatgcgc
    30601 cagatggctg caatagctcg ccagccggtg cgcaagccag taatcggggc gatgtaaaaa
    30661 gaaggtcaga cactgcggat gggtgacttc aatacgcttc acgctaataa ataattgatc
    30721 cagtgctggc agttgtaaca gcatcaataa tcgctggtgt aagtgtgagg cttttactgc
    30781 atcgccgtta tgccagtgta gggttgaacg aagataaaag tcccagcgta acccgtcagc
    30841 agaggtttcc cagtgatgcg ctaaatcgcc aatcgggcgc tgggtattat tatcgaagcg
    30901 ggtcaggccg gaaaatatct gtccggcgag atgctgctcg gcacggccgg gcaaaaagcc
    30961 cggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggcg tatcgttttg
    31021 ccattgtccg cccataaacg gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc
    31081 cagctccagc acatcttgct gctttccggt ttccagcgcc tgttccatca tcgcattgcg
    31141 tagcgattct ggcgtgacca gaaagcgtag ttgcccgcgc tttccgcgtc ctgactgcgc
    31201 ctgccactcc agccatcccg cctcctgtgc ctggcgtaac agcgtacgaa catggcgttc
    31261 gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct ttccggcgga
    31321 aggttgccac agacgttgat actggttaag acggttgagc agtcgcatat aaacccggaa
    31381 caatattatt taactattca ctattacttc cgtatatatc aggtgatact caatcaccat
    31441 taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga tatggatggc
    31501 actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt ggcgcgactg
    31561 cgtgaacgcg acattaccct cacttttgcc acggggcgtc atgcgctgga gatgcagcat
    31621 attctcgggg cgctatcgct ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat
    31681 tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc ggagctggtg
    31741 ctgtatcagc aatgggatac ccgagccagc atgcatatct tcaatgacga cggttggttt
    31801 accgggaaag agatccctgc gttgttgcag gcatttgtct atagcggttt tcgttatcag
    31861 ataatcgatg tcaaaaaaat gccactcggc agcgtcacca agatctgctt ctgtggcgat
    31921 cacgacgatc ttacacgctt gcagatccag ctatacgaag cattaggcga gcgtgcacat
    31981 ttgtgttttt ccgccacgga ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct
    32041 gcattgacgg tgctgaccca acatttaggt ttatcgttgc gcgattgcat ggcctttggt
    32101 gatgcgatga acgatcgcga aatgttaggc tgcgtcggta gcggatttat tatgggcaat
    32161 gcgatgccgc aattgcgcgc ggagctcccg catttaccgg tgattggaca ttgccgaaat
    32221 caggctgtct ctcactattt gacgcactgg ctggactatc cacatctacc ttattccccc
    32281 gaataacgag atcccttcca gcatcgggca attgcccggt tttttttgcg ttgaatttgt
    32341 cattttgtgc cgtggtgttt aaaccgcaca gaataaattg tcgcgatttc acctttaaaa
    32401 tagaattaaa agagaaaaaa attctccgtg gaagggctat gttagataaa attgaccgta
    32461 agctgctggc cttactacag caggattgca ccctctcttt gcaggcactg gctgaagccg
    32521 ttaatctgac aaccaccccg tgctggaagc gtctgaaacg gctggaggac gacggtatcc
    32581 ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg
    32641 tgctgataaa aacgcaacat cacagcagcg aatggtattg ccgctttgtc acggtggtta
    32701 ccgaaatgcc agaagtgctg gggttctggc gcatggccgg tgaatacgat tatctgatgc
    32761 gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtctg gtaaacagcg
    32821 tgccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa tacaccactt
    32881 ctttacccat cgaataaata tccagaatca ggtcaggaca caacgcgtgc gattatttgc
    32941 tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct
    33001 tgtcattatc gcgatgctgc aactggttcc gccaaaagtg gttggtattg ttgtcgatgg
    33061 cgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca ccatggtgct
    33121 gattgccgtt gtggtttatc tcctgcgtta cgtctggcgc gtattgctgt ttggtgcgtc
    33181 ttatcaactg gctgttgaac tgcgtgaaga ttattaccgc cagctaagcc ggcagcatcc
    33241 tgagttttac ctgcgtcatc gcacgggtga cctcatggct cgtgcgacca atgacgtcga
    33301 tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg gtggattcac tggtaatggg
    33361 ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct tatttgccct
    33421 gttgccgatg ccagtgatgg cgatcatgat taagcgcaac ggcgatgcct tgcatgaacg
    33481 ctttaagctg gcgcaggcgg cgttttccag tcttaatgac cgcacccagg aaagcctcac
    33541 cagtatccgc atgatcaaag cttttggtct ggaagatcgc cagtcggcgt tatttgccgc
    33601 ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga
    33661 cccgaccatc tatatcgcga ttggtatggc gaacttgctg gcgattggcg gcggtagttg
    33721 gatggtggtg cagggtagtt taacgctggg ccagctcacc agttttatga tgtatttagg
    33781 tctgatgatt tggccaatgc tggcgctggc atggatgttt aacatagtgg aacgtggtag
    33841 tgctgcgtac agccgcattc gcgcgatgct ggcggaagcg ccggtggtga atgatggtag
    33901 cgaacccgtg ccggaaggtc gtggcgaact ggatgtaaat attcgccagt tcacgtatcc
    33961 gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct
    34021 gggtatctgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca tccagcgtca
    34081 tttcgacgtc agcgagggta atattcgctt tcatgatatt cctctgacgc agttgcaact
    34141 cgatagctgg cgtagccgcc tggcggtagt tagccagaca ccgttccttt tttctgacac
    34201 cgtagcgaac aacatcgcgt tgggttgccc gaatgccacc cagcaagaga ttgagcatgt
    34261 cgcgcggtta gccagcgtgc atgacgatat tttgcgttta ccgcaaggtt acgatacaga
    34321 agtgggcgag cgcggcgtga tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg
    34381 tgcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg cagtggacgg
    34441 acgcacagag caccagatcc tgcataacct gcggcagtgg gggcagggaa gaacggtaat
    34501 catcagtgcc catcgccttt cggcactgac ggaagccagt gaaattattg tgatgcagca
    34561 cggacatatc gctcagcgtg gcaatcatga tgagctggcg caacaaagcg gctggtatcg
    34621 cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg aaattcgcga
    34681 ggaggccgtt gatgcgtagt tttagccaac tgtggccgac tctcaagcgt ctgttagcgt
    34741 acggttcacc gtggcgtaaa ccgttgggga ttgcggtcct gatgatgtgg gttgcggcgg
    34801 cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg gtagcgaaaa
    34861 ataacctgcc gttgaaagtg gttgcagggc tggcggcggc gtatgttggg ctgcaactgt
    34921 ttgcagccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag
    34981 tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg ccagccatta agcgagtttg
    35041 atacccaacc tgtcgggcag gtgatttccc gcgtcactaa tgatactgaa gtgatccgcg
    35101 acctctacgt taccgtagtg gcaacggtcc tgcgtagcgc cgccctggtg ggtgcgatgc
    35161 tggtggcgat gtttagtctc gactggcgaa tggcgctggt ggcgataatg attttcccgg
    35221 tggtgctggt ggtaatggtg atataccagc gttacagcac gccaattgtc cgtcgtgtgc
    35281 gcgcctatct ggcggatatt aacgacggct ttaacgaaat catcaatggc atgagcgtca
    35341 tccagcagtt ccgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac
    35401 actatatggc gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt ccgctgctga
    35461 gtctgttttc atcgctcatt ctttgtggat tgttgatgct gtttggcttc tccgccagcg
    35521 gtaccattga agtgggcgta ctgtatgcgt ttatcagcta tcttgggcgg cttaacgaac
    35581 ccttaatcga actgaccacg caacaggcga tgctgcaaca ggctgttgtt gctggtgagc
    35641 gcgtgtttga gctgatggac ggaccgcgcc agcaatatgg caatgatgat cgcccgttac
    35701 agagtggcac catcgaagtc gataacgtgt catttgctta tcgcgatgac aatctggtgc
    35761 taaagaacat taatctctct gtgccttcgc gcaattttgt ggcgctggtc gggcataccg
    35821 gcagtggtaa aagtaccctc gccagtttat tgatgggcta ttacccgcta acggaaggtg
    35881 agattcgcct tgatggtcgt ccattaagtt cgctaagtca cagcgcgctg cgccagggcg
    35941 tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc
    36001 tggggcgtga tatctccgaa gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg
    36061 agctggcacg cagtatgagc gacgggattt atacgccgct gggcgagcag ggaaataatc
    36121 tctcagtcgg gcaaaagcaa ctgctggcac tggcacgcgt gttggtggag acgccgcaaa
    36181 tcctgatcct tgatgaggca accgccagca ttgactccgg tactgaacag gcgattcaac
    36241 atgctctggc ggcagtgcgt gaacatacca cgctggtggt gattgctcac cgcttatcaa
    36301 ctattgttga tgccgacacc attctggtgc ttcatcgtgg gcaagctgtg gagcagggca
    36361 ctcaccagca actgctggcg gcccagggac gctactggca gatgtatcaa ctgcaacttg
    36421 cgggcgaaga gctggcagcc agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc
    36481 aatatttcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat
    36541 tttcaatctt cttaacttcc tgctctcttt ctcatttttc atttctggca caccgcttgc
    36601 aatatcttct tcgtgtagca gaaccattac cgaattctga ccggagggga tctatgaagc
    36661 tggtgaccgt gataatcaaa ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca
    36721 ttggtattca gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg
    36781 ccgagctgta ccggggggcg gaatacagcg tcaactttct gccaaaagta aaaattgatg
    36841 tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca
    36901 ccggaaaaat tggcgacggc aaaatctttg tcgctgaatt gcaacgcgtc attcgtattc
    36961 gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac aggaacgaaa
    37021 aatgaagata gcgacgataa aaactgggct tgcttcactg gcgatgctgc cgggactggt
    37081 aatggctgca cctgcggtgg ccgataaagc cgacaatgcg tttatgatga tctgtactgc
    37141 gctggtgctg tttatgacta ttccggggat tgccctgttt tacggtgggt tgattcgcgg
    37201 caaaaacgtg ctgtcgatgc tgacgcaggt gacggtgacg tttgcactgg tctgtattct
    37261 ctgggtggtt tacggttact cgctggcgtt tggtgagggc aacaacttct tcggcaacat
    37321 taactggctg atgctgaaaa acatcgaact gacggcggtg atgggcagca tttatcagta
    37381 tatccacgta gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga tagttggggc
    37441 gctggcggaa cgaatccgct tctcagctgt gttgattttc gtggtggtat ggctgacgct
    37501 ctcctacatt ccgattgcgc atatggtgtg gggcggtggt ttgctggctt ctcacggtgc
    37561 gctggatttc gcgggtggca cggtggtgca cattaacgcc gcaatcgccg gtctggtggg
    37621 cgcatatctg ataggcaaac gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct
    37681 gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct ttaatgccgg
    37741 gtcagcgggc acggcgaatg aaatcgcagc actggcattt gtgaatactg tggtcgcaac
    37801 ggcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta agccttcact
    37861 gctgggagcg tgttcaggcg cgattgccgg tctggtcggc gtgacgccag cctgtggcta
    37921 cattggggtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg
    37981 cgttaccatg ctcaaacgct tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca
    38041 cggcgtttgt ggcattgtcg gctgtatcat gaccgggatt tttgctgcca gctcgctggg
    38101 cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag ttgctggtac agctggaaag
    38161 catcgccatt acgatcgtct ggtccggtgt ggtggcattt attggctaca aactggcgga
    38221 tctgacggtt ggtctgcgtg tgccggaaga acaagagcga gaagggctgg acgtcaacag
    38281 ccacggcgag aatgcctata acgcgtaaca agcactgcaa aaaacagccg gacggttttc
    38341 acctccggct atttttttaa ttgtgattac gcattacccc ttcctgaacg gtcgaggcaa
    38401 ccagcacgcc gtcctgagta taaaactcac cgcgcacaaa gccgcgtgcg ctggacgccg
    38461 aggtgctctc cacgctatac agcagccatt cattcaaatt aaacgggcga tggaaccaca
    38521 tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct
    38581 gtagagctac cggcaggaag ttaagatcag aagcgtaacc gagcagatac tgatgaacgc
    38641 gcagatcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgc ggttctgcga
    38701 cgtgcccttt cagtgggtta tgaaactcca ccggacggac ttcaagcgga cgatcgcaga
    38761 tgaatttatc tttcagcact ggcggcagca ggtgcgccag cgattgggcg atttgcgttt
    38821 ccgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt tcgaaacccg
    38881 cttctggtgc ctggaaagag gcagtcatat aaaaaatcgg tttgccgttt tgaatagcag
    38941 caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca taaataatcg
    39001 gtttcttact atcgcctggg cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt
    39061 cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg ccaaacacct
    39121 ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt ttttccagat
    39181 ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt aacaaagctg
    39241 ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ccactggtcc gatgggtgca
    39301 atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca cacttgttga
    39361 cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg tgcattgata
    39421 ataaggagaa gtgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt
    39481 tggcggcttg cgcagataaa agcgcggata ttcagacgcc agcaccggct gcaaatacgt
    39541 ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt accgtctgga
    39601 tccgtcagaa agtcgcactg ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt
    39661 cgttagccga tgcaccgtca aaagtactgg cgcagaaagc ggtgcgtacc gaaggcaaac
    39721 agtcaccatt cagctttgtt ctgccattta acccggcaga tgttcagccg aatgcgcgta
    39781 ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc gataccgttc
    39841 agccggtgat caacaagggc ggaactaaag ccgacctgac attggtgccg gtgcagcaaa
    39901 ccgccgtgcc ggttcaggcc agcggtggcg caacgactac tgtaccttcg acttcaccaa
    39961 ctcaggtgaa tccgtcttcg gcagttccag ctcctacgca atattaagcg cagttaaccc
    40021 tctccgcccg gagagggtta gtagttccag cgataacgct gcaagtcgat ttgcccgctt
    40081 cccgatacca tcacaccttc tgccagtaat gcctgtcgtt gacgctgtaa atccggtccg
    40141 gttagcgaaa ttgtgccgtg gcgattaacc acccgatgcc agggcaaggt gctgccttca
    40201 gggagacgct ttaacacacc gcccacctgg cgcgcggcgc ggggcgatcc tgccagtttc
    40261 gccacatcac cgtaagtggt gacatagcct tcgggaatag cggcgacgat ttgccagacg
    40321 cgttggggaa atgaatcttc tttttccatc ttttcttcct gaggtaattt ttcagcataa
    40381 tctggaaaaa cgcccgagtg aagtcgcatt gcgcaagaaa ccagcatccg gtacgcaatg
    40441 ggttgcaatt agccggggca gcagtgataa tgcgcctgcg cgttggttct caacgctctc
    40501 aatgggggct ctgttggttc tcccgcaacg ctactctgtt taccaggtca ggtccggaag
    40561 gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc tggcagggcc cccacccatt
    40621 tctgcctccc cccgtttcgt caaaaaatcc caatatggct aaactttaac cacaactgac
    40681 gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt atgtctgatg aagtatgttg
    40741 atggttttgt ggttgccgtt cctgccgata aaaaggatgc ctatcgggaa atggctgcta
    40801 aggctgcgcc attgtttaaa gagtttggcg cgcttcgtat tgtcgaatgc tgggccagcg
    40861 atgtaccgga tggcaaagtg accgattttc gtatggcggt aaaagctgaa gagaatgaag
    40921 aggttgtctt tagctggatt gaataccctt caaaagaggt ccgcgacgct gctaatcaaa
    40981 agatgatgtc ggacccacgg atgaaagagt tcggcgagtc catgccgttt gatggtaagc
    41041 gaatgatcta tggcggattc gagtcaatca tcgacgaata ggtagcgtga cgggctgtgc
    41101 ggcgtgccgt cagccctaca attcacaaat gttgctcggc ccagcgtaag aaatcttctt
    41161 tcggtaatgc cttgctgtag agccagccct gaccgtagtg cacgccatgc tggcgtaacc
    41221 actcttcttg tttactggtt tcgattccct ccgctaccat tttcagtttc agtgtttttg
    41281 ccatttcgat gatatgcggc gtgacatttt tatattccag cgcatcaacg aaagatttat
    41341 cgatcttcag aatgtcgaca tccagatcct gtaaataact taaacttgaa taccccgtac
    41401 caaaatcatc gagataaatt tcatggcccg cctcccggta gcgagaaatt atcggggcgc
    41461 tggttttcgg atcggcaaac tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa
    41521 cctgatagtg attgatcatt tcacgcagca attgcgggat tttttccgag gtgagcacgg
    41581 tggattcaag attgatcgaa atatgctgct gcggatgctg acgcagccag tcgcccatat
    41641 cttcgaagac gcttcttata atcagtagcg tcaacggctc agaaaggccc gtttgctgtg
    41701 ccagtggaat aaaactatct ggtgacaacc aactaccgtc tgtctgcggc cagcgcgcca
    41761 gtgcctcagc accgacaatt ttgccattgg ctaaggagac aatcggctga tagtgcacgc
    41821 aaatatcacg attttcgata gcatcctgca gccgatgatg cggtgactgg atacggcgca
    41881 aaatacgcag cacaaacatc gctgccagca ggccaatcac caacccgagc ggtaaccaaa
    41941 taaagacttg ccgatgccag cctttctgca acattttcgt tgaagcccag gtgatgatcg
    42001 aaatattcat ttccgggaag ggcaggatat cgtaaatgat tccattattt tcgatatgct
    42061 caccgggtgt tttttgtagt ctggtaataa ttccctgagc aatttcatcg ctgctggtta
    42121 tcacaacgtt atgggcattg ccaataatgg cggcatcaat ttgccatgag ctatagggaa
    42181 tgacatcaat aaaggaagcg gggtcgatca tgacgacata atgtgccgtt cccatggcaa
    42241 ccatgtaacg gataatgcct aaatcgttat gcgatgttaa ccagacacga taaccatctt
    42301 tcgaaatttt acctggctcg gggaaggtat cgggcgggct ttcatgctcc agagacgaac
    42361 actggggaac gttgttatcg atataaacca cttcctgaat atagcgataa ctgtaagata
    42421 cccgacgcat ttccatgaga tgggcttcgc tacaggtagc gccttgccat ctttccagct
    42481 cttgcagcgc atctttccct tgtgtcgcca ccttattggc ccgaatagcg acgcgggagg
    42541 aatacgtatt cagctcttca ataaatgatg tttctacctg ctgatgggcc agccagatgc
    42601 ttaagccgac aggcagcaat actgaaagaa tcagtactcc cgaaataagg ccgaccagat
    42661 gtcgtgttct caccagcata tccttattaa cacagtgtag ggaaagtata tctgattgtg
    42721 gaaaaaggct ggcaggagat aaggaggtag agccgggatt tcatgaaggg atgggtggaa
    42781 acgaacgttg tcatttgacc aggcgcagag ctttacgcct ggtatgtaac cctaacttaa
    42841 gatgttgatt cggcgcgagc gagggtaaaa atatcgtaaa aagacagttc acctttacgg
    42901 acgatcattt tttgcagttg ttctttatga acatccgcga cacgcggaga gttcaagatg
    42961 gcttcaacca gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg
    43021 cggaaatcca gcacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag
    43081 aaaccattaa gcaggataaa taatacaacc agtagccaga cagagcctga cagcgtttcg
    43141 gactgcaaca tcactgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc
    43201 ggatgtttac ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta
    43261 tttagagact caattgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt
    43321 ggtatctgca actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta
    43381 acagatttac cacaattttg cataacagtt gcgaaaaaca tattacaatt ttttaattat
    43441 tctggctgga ttaccgccca cgacaacgtt gtccgggaca tcttttgtga caactgcacc
    43501 tgaggctacc acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc
    43561 aatccagacg ttattaccga tggtgacggg tatccccagt tcagcaccgc tattacgtgc
    43621 tacagggtcg atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt
    43681 atcaccgatg cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt
    43741 attgccgaga aaaatattat agccataatc acagcgaaac gttggctcaa tataagcctc
    43801 tgtcacctga ccgaataaat cagcgagaat ttgctggcgt aatgtgtgct cttccgccag
    43861 ggaatgattg tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc
    43921 atctgccgag cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga
    43981 cctccggttg ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa gagaacgttc
    44041 ccattacatc tgtgtgataa agatcacata gggtacgtct tcatggcgaa aaagtataaa
    44101 attcttaata aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt
    44161 ccatactgag gaagggatct tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg
    44221 atctgcggct gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc
    44281 gataacacgc tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt
    44341 ttttgttaaa ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata
    44401 aactcgcagg attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact
    44461 tccgccattt ctgaagatcc tgcatattaa taccataact gctgaacaac ataaaggtgt
    44521 catccagata ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt
    44581 aattaagtgc gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg
    44641 aggttgggtc gttaacccag ccatgattgc tttcttcaag gtttgcaaga cagtcatgat
    44701 acagggtttc acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat
    44761 ccataacgcg tccccttctt agtggttgaa ctaacggaca cctttcggga tggaaaaaac
    44821 ttactgacct ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt
    44881 tttcatcgca ttattacgaa aaattacaat taggaaggaa gcgggacagg acttactttt
    44941 tcatactagc actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt
    45001 caggattatc gccagatgaa attggtaaca ctaataccag gattgc
//
LOCUS       DAYPHN010000018        44341 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000018 DAYPHN010000000
VERSION     DAYPHN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.018"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(135..1139)
                     /gene="argF"
                     /locus_tag="ACG0BW_001017"
     CDS             complement(135..1139)
                     /gene="argF"
                     /locus_tag="ACG0BW_001017"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFK7258011.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1301..1717
                     /gene="rraB"
                     /locus_tag="ACG0BW_001018"
     CDS             1301..1717
                     /gene="rraB"
                     /locus_tag="ACG0BW_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFK7258012.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1763..2266)
                     /gene="yjgM"
                     /locus_tag="ACG0BW_001019"
     CDS             complement(1763..2266)
                     /gene="yjgM"
                     /locus_tag="ACG0BW_001019"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7258013.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2459..3655
                     /gene="yjgN"
                     /locus_tag="ACG0BW_001020"
     CDS             2459..3655
                     /gene="yjgN"
                     /locus_tag="ACG0BW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFK7258014.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGAV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3711..6566)
                     /gene="valS"
                     /locus_tag="ACG0BW_001021"
     CDS             complement(3711..6566)
                     /gene="valS"
                     /locus_tag="ACG0BW_001021"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFK7258015.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADNKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6566..7009)
                     /gene="holC"
                     /locus_tag="ACG0BW_001022"
     CDS             complement(6566..7009)
                     /gene="holC"
                     /locus_tag="ACG0BW_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFK7258016.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7363..8874)
                     /gene="pepA"
                     /locus_tag="ACG0BW_001023"
     CDS             complement(7363..8874)
                     /gene="pepA"
                     /locus_tag="ACG0BW_001023"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFK7258017.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9028..9078
                     /gene="ytgA"
                     /locus_tag="ACG0BW_001024"
     CDS             9028..9078
                     /gene="ytgA"
                     /locus_tag="ACG0BW_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFK7258018.1"
                     /translation="MSIIILATISTTQENK"
     gene            9141..10241
                     /gene="lptF"
                     /locus_tag="ACG0BW_001025"
     CDS             9141..10241
                     /gene="lptF"
                     /locus_tag="ACG0BW_001025"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFK7258019.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10241..11323
                     /gene="lptG"
                     /locus_tag="ACG0BW_001026"
     CDS             10241..11323
                     /gene="lptG"
                     /locus_tag="ACG0BW_001026"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFK7258020.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11484..12986)
                     /gene="yjgR"
                     /locus_tag="ACG0BW_001027"
     CDS             complement(11484..12986)
                     /gene="yjgR"
                     /locus_tag="ACG0BW_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFK7258021.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTVSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(13064..14062)
                     /gene="idnR"
                     /locus_tag="ACG0BW_001028"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(13064..14062)
                     /gene="idnR"
                     /locus_tag="ACG0BW_001028"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFK7258022.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(14129..15448)
                     /gene="idnT"
                     /locus_tag="ACG0BW_001029"
     CDS             complement(14129..15448)
                     /gene="idnT"
                     /locus_tag="ACG0BW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFK7258023.1"
                     /translation="MPLIIIAAGVALLLILMIVFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(15512..16276)
                     /gene="idnO"
                     /locus_tag="ACG0BW_001030"
     CDS             complement(15512..16276)
                     /gene="idnO"
                     /locus_tag="ACG0BW_001030"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFK7258024.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(16300..17331)
                     /gene="idnD"
                     /locus_tag="ACG0BW_001031"
     CDS             complement(16300..17331)
                     /gene="idnD"
                     /locus_tag="ACG0BW_001031"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFK7258025.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            17548..18111
                     /gene="idnK"
                     /locus_tag="ACG0BW_001032"
     CDS             17548..18111
                     /gene="idnK"
                     /locus_tag="ACG0BW_001032"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFK7258026.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(18115..19134)
                     /gene="ahr"
                     /locus_tag="ACG0BW_001033"
     CDS             complement(18115..19134)
                     /gene="ahr"
                     /locus_tag="ACG0BW_001033"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFK7258027.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADY"
     gene            19330..19414
                     /locus_tag="ACG0BW_001034"
     tRNA            19330..19414
                     /locus_tag="ACG0BW_001034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19364..19366,aa:Leu,seq:caa)
     gene            19951..20523
                     /locus_tag="ACG0BW_001035"
     CDS             19951..20523
                     /locus_tag="ACG0BW_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258028.1"
                     /translation="MRTQEGLKAIYEARWSRYEKHLHAGMDTTSIILKGHLFVEELLF
                     EILKLHCRDSGPIENIKLGFSHKLNLVHAMFGSHLPGMEFPKNVWAALDKLNKLRNAL
                     AHSIDSPRAAEVFKSFVSSYSQLVGREVQVRITDEIPGIDRKLGLELIGIIMYLLGFL
                     GCLHGIAYLNPPLMYGSEIPVTIPNKIEHE"
     gene            20605..21375
                     /locus_tag="ACG0BW_001036"
     CDS             20605..21375
                     /locus_tag="ACG0BW_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFK7258029.1"
                     /translation="MEYVAFGDESGTTGSDRCYGIGLLCVRKDTLHIFNERVQKLKDK
                     YGIVGELKWSKIKNSAGQANICIELLAMVLKNSCCFHSIIVVKNMYNNWQTDRELAFY
                     QTYTMLVKNVAKQIKSDVEVIIDQKIDKYKKNDEVTGIVANHMLARAGMKKLVKSVTM
                     HDSKHHLGLQVVDILTGAVNSGYLKYLNPRLELSVAKELAFERMAALLGWDVFHYDTF
                     PNKDFNIWHFPQEMRAMPGSKSIRPDYSVPLVKREELA"
     gene            21414..21824
                     /locus_tag="ACG0BW_001037"
     CDS             21414..21824
                     /locus_tag="ACG0BW_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258030.1"
                     /translation="MSYDDLDPATKRVLQQAEYMRSNEAKLAQIACIKQLVAYTNWCA
                     ERGDFGDPNPATKEDSLKLLHVRQMRIGYDTRQVLECGFEGLYEHIDNALENALAWRD
                     YRVKEWAAESDIAELNALWEWFRERLPADYVSPY"
     gene            22384..23016
                     /locus_tag="ACG0BW_001038"
     CDS             22384..23016
                     /locus_tag="ACG0BW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFK7258031.1"
                     /translation="MAHNTLIYCRASTAEQHADRALGLLREFASDRGWPVRAEYIENA
                     SGARLARPELMRLLDDAQAGEVLLVESIDRLSRLTQKEWATLKQRIQDKGLVIVAVDL
                     PTSWQLLNDTASELTEGILRAVNAMLIDILATMARVDYETRRARQAQGIERAKAEGKY
                     NGKSKDESAREIVRAMLTVGNKPEHIMKAAGISRATFYRIKRELSASVTE"
     gene            24276..26120
                     /locus_tag="ACG0BW_001039"
     CDS             24276..26120
                     /locus_tag="ACG0BW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFK7258032.1"
                     /translation="MPTNLRRLLAQAADADPLNTADADVQQMIADGVVIRRSKICPVK
                     RKPVKAAYNTKTEEFYYNADGSAMTLREWRTLHGVQPKKQKQRSDAGLRQVIQPGDAI
                     HCRRAEIEQWLNKKPARYVEPLPPDFPGVRVDDAALKPREHAVPESTTGQNAFLKACR
                     EFLICDEKTMRRINRGMSKLEAMRNADFKRYGDSGEFRQFWCGESKPTEDRFNSTNHH
                     CDPNGLAASPSVPPLNTTPYEFRPLARCDRNARVSLLPPDGLEWRPGQLSVADAIFSR
                     GSDVLAVLPTGYGKSAAFTYPLIYESRAGVGLSVIVVPTNTLKRQILRQLREAGVHAV
                     AFGDKLPRHRNAQQLAGLAAGAFRVAVVSPEHLVPSSQVLAALMAYGVDRFIIDEAHT
                     VKDWDFRPAYDAMQHTLLNAFPSAQRCVFTATLLRENERSLMESLGCRGDYVTFRADV
                     ARENIQLTPVRQCRERFRAAIGLVEALPKEEKTLILVNDLKTLRAYLKLLGDRARPYH
                     SKTMHLSNEERHANAEWFASTPGAILIATKAFGLGADVPDIRNVVVTYVPDDLPDLVQ
                     MIGRAGRDGEPARAFLLWSSLALDVAPDELAYFYRPENCNWQYIAKYL"
     gene            26158..26595
                     /locus_tag="ACG0BW_001040"
     CDS             26158..26595
                     /locus_tag="ACG0BW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258033.1"
                     /translation="MPVTEHAQRRAARADALITAQLAFALNGYNWDELAEPHRKTLNR
                     EAIIREVSTPLERYCLLNDCEPWECLAAVLNRMKRPDGSRRWKAIRLSDRIFNGRVHP
                     VRKTPDEVYAAIARRLFLPKPVHQEVIQHIRALGLLPVGDNHQ"
     gene            26592..26801
                     /locus_tag="ACG0BW_001041"
     CDS             26592..26801
                     /locus_tag="ACG0BW_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258034.1"
                     /translation="MTKSEFEAGAWELKTLLLGALTRRLKEDEAGESRMAAAELSSCV
                     NFLKQNAADSPDTTFNNVFPEDDDQ"
     gene            26798..26974
                     /locus_tag="ACG0BW_001042"
     CDS             26798..26974
                     /locus_tag="ACG0BW_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258035.1"
                     /translation="MTPEQFRQWFATLPEKYQRVVRFMYDNARKSGTHTASSLALIHT
                     LVTDQIKFDSEFNL"
     gene            26971..27534
                     /locus_tag="ACG0BW_001043"
     CDS             26971..27534
                     /locus_tag="ACG0BW_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258036.1"
                     /translation="MTLSITDIQRINALAHKHLKNNEEIAAETGHTLTDIDAVIRGVY
                     VDDLAEHWKDDADINLAEYNAIRSRYKPGKLHRDTEFMIARDMLIRPETVLAIAHSRF
                     SNAHTHPVQKPLKVKPAKPKVVRKLVPTDLDEIHKLERFGWPAAEIAGKLGVPVEVVA
                     HVQRCLRNRWSYPDTLESVNSKFSEAE"
     gene            27531..27767
                     /locus_tag="ACG0BW_001044"
     CDS             27531..27767
                     /locus_tag="ACG0BW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258037.1"
                     /translation="MMAENEPLHPAVNPDLYVNLDEQREGYAPIESRGWDVLTQNLTG
                     KGRTDPSQTLNQNPKQRPRGSVGERRWLRKRGLL"
     gene            27780..28019
                     /locus_tag="ACG0BW_001045"
     CDS             27780..28019
                     /locus_tag="ACG0BW_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258038.1"
                     /translation="MSKTIVLLPPRKNTDWAAQLKLISESLEVSQADLAHAYQVDRRD
                     MGKAYHGVRKLPERCVPVHMLLLAQVHDLRALSGE"
     gene            28023..28703
                     /locus_tag="ACG0BW_001046"
     CDS             28023..28703
                     /locus_tag="ACG0BW_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258039.1"
                     /translation="MSDQNVNPYLSGADAASQQSPYMAAPVGADNGASRFSSAPADAD
                     FLRTQPVNGNDGGQWNNGNIANQQDQNADSSGAQVGDTLQLQDGTTVELTPELHGLMG
                     QFHQSVESQLGQGTVGAMLQYAGKLDAGTQSTLERMVMSGDPAQMSRAVQYLQERMAG
                     GSITGYVPSGQTAAQPVGMSHAEFIAAYDQLSQWAQQSGYGPGHRQYDEQYRALVAQR
                     SAGKRAGR"
     gene            28843..29256
                     /locus_tag="ACG0BW_001047"
     CDS             28843..29256
                     /locus_tag="ACG0BW_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258040.1"
                     /translation="MSTCNFHAVERVADVRPVFALPLGRCDHIYPERVQHVFNPDEAV
                     YIVARSATSGTRTSGVSRGLSPVLPPKMAERMRERKRHEKNRARPRKVITEAIRADII
                     DRSAKGQKNCYIASKVGVSETSVRKVLNMARMSAY"
     gene            29454..30449
                     /locus_tag="ACG0BW_001048"
     CDS             29454..30449
                     /locus_tag="ACG0BW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFK7258041.1"
                     /translation="MADTSYSPDLFRPHYGGVESDNDIHIEAYDRDVQTRLESKSVFL
                     AEGLSTYRPLKASNQYRIDRLSGDLEVKGRTAGQSLEPTAVKSDKLIISVDTLSYIRI
                     PIDYQDDWTAPTFRSELSRNIGTAHAKFYDEAHTIQLIKATDFVPPPTLQPTFNPLTD
                     NVVPLNLTTGSEEDAANELVHAIKAAANRFLTEKDIPVAELVLLIDPAWFSILMEHKK
                     LMNVRYSRDSGNDYAWRRVGYVAGVRVIELNSYPQEAITGHELGADYDVTAEEALTRA
                     TLFKPSSVLLTVEAQSVINRQWDDEREMTMVMDSYRLFNVGLRRPDCVINFRQAA"
     gene            30466..30975
                     /locus_tag="ACG0BW_001049"
     CDS             30466..30975
                     /locus_tag="ACG0BW_001049"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFK7258042.1"
                     /translation="MVNDAYAPELLRSLFLYDPVTGRLHHKANRRRVKAGSYADSTRR
                     ADGYRQVALRLDGKQYQLKAHRVAWILAHGAIPDGLQVDHINGIRDDNRLCNLRLVTQ
                     RENDQNRRKARGYSWNKGCSKWEAYIRVDGVLRYLGLFTTEAAARAAYLKAKARYHPT
                     TPADLLRAA"
     gene            30995..31429
                     /locus_tag="ACG0BW_001050"
     CDS             30995..31429
                     /locus_tag="ACG0BW_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258043.1"
                     /translation="MADEKRIRQIRALADDDPRRQRYVELLAQAGRMTIPRLHPGDAN
                     SVMSQLATMLDAPVPIVGAMTEHAAAWRACLLEQGLGDLLAGLSKQVLTIFFLLCWRD
                     SALRLAGKQKELLDIAIEGKTLKQASEARANDIKRNAYGIDD"
     gene            31413..31712
                     /locus_tag="ACG0BW_001051"
     CDS             31413..31712
                     /locus_tag="ACG0BW_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258044.1"
                     /translation="MALTIKLRWRYIEPPPMTGALADLKVWVMDTGEPELEAEFRMLL
                     GLMRRNGISDEHVNAMAVELYALVCQRQREEYEACKRAASDSGDFESWLHGQTDY"
     gene            31685..32116
                     /locus_tag="ACG0BW_001052"
     CDS             31685..32116
                     /locus_tag="ACG0BW_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HFK7258045.1"
                     /translation="MVTRTDRLLRKAMRSYLEHLGKMPLAERITNAAAFEDVYRFLLN
                     GSGRLELRRADVAAVRVFLWNYQYRRCAVTGKPLRLTSAVLDHCHRTGRVRAVVHRSA
                     NAAEGGRYVGLTCGMSVIRLGAMVDMYRKIYKGINIIYPNK"
     gene            complement(32161..32805)
                     /locus_tag="ACG0BW_001053"
     CDS             complement(32161..32805)
                     /locus_tag="ACG0BW_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258046.1"
                     /translation="METINTKETCPSEFYIFVNDMNPITHNNVNIVGSISSAAISALA
                     MNGNNPQKYIDGDLDYLPFLPNENMFPALSMYHVSAINDYRIEYNAEINRKAYFKTYP
                     SRLSAVYAFGDYDTCVEVHQRHNWDLNTVRRFRLKDLPYTRVAKVNMEIVSLMRHAYK
                     ISTFESDYEVWQHYWSGQGNLALQIPDRDFKPQRIESGVIWEYLIEGALELIRE"
     gene            complement(32827..34053)
                     /locus_tag="ACG0BW_001054"
     CDS             complement(32827..34053)
                     /locus_tag="ACG0BW_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFK7258047.1"
                     /translation="MSFKRDRYLVLDPCGNYLVRIAIPSYMRHLFQGKRYFMKSTGTR
                     DIRQARLFRDAIALEWTRLRNLLKPQGGSSVDQIIDELRRVSVYAKEAPASFGASVQA
                     CPSLLKMRDLYLLQYSEKRKLTTLSKTNKAVEVLLTHLKKKDIQLRDINRTIVTGWLD
                     KLKTERAPQTIQNYISALAQIWDLARNRYHDAPQDNPWRGHALEAKSSKVSYEVFAPG
                     ELAKIYALLDDEMKAVTAIGAYSGMRLNEICTLRESSIKNVEGVLCFEITEGKTKSAA
                     RIVPVHSKIIPLVKSLLKTTHSGFLFYHASITDRADGKRSTWHTQQFTRAKRKALGET
                     YTQRKVFHSLRHEFVHQLDRAQVPEDRIALLVGHERGVTESFKTYSKNAVSAQEVKEY
                     VELVKHVFDLPIYNKL"
     gene            34460..35668
                     /locus_tag="ACG0BW_001055"
     CDS             34460..35668
                     /locus_tag="ACG0BW_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7258048.1"
                     /translation="MTDGGGMHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLAD
                     ARVRRDETRKLLANGVDPGDKKKNDKVEQSKARTFKEVAIEWHGTNKKWSEDHAHRVL
                     KSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSG
                     LIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFI
                     RSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGSKMRTTHLVPLSTQALAILK
                     QIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRTMACSSLVE
                     SGLWSRDAVERQMSHMARNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFE
                     YAKINNPLKQ"
     gene            complement(35985..37165)
                     /locus_tag="ACG0BW_001056"
                     /pseudo
     CDS             complement(35985..37165)
                     /locus_tag="ACG0BW_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            37752..37981
                     /locus_tag="ACG0BW_001057"
                     /pseudo
     CDS             37752..37981
                     /locus_tag="ACG0BW_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(38495..39841)
                     /gene="insG"
                     /locus_tag="ACG0BW_001058"
     CDS             complement(38495..39841)
                     /gene="insG"
                     /locus_tag="ACG0BW_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFK7258049.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGDLYSHRWEIKLGYREIKQTMQRSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            40450..41667
                     /gene="nanX"
                     /locus_tag="ACG0BW_001059"
     CDS             40450..41667
                     /gene="nanX"
                     /locus_tag="ACG0BW_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7258050.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFIFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            41679..42797
                     /gene="nanY"
                     /locus_tag="ACG0BW_001060"
                     /gene_synonym="yjhC"
     CDS             41679..42797
                     /gene="nanY"
                     /locus_tag="ACG0BW_001060"
                     /gene_synonym="yjhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,7-anhydro-N-acetylneuraminate hydratase"
                     /protein_id="HFK7258051.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            42840..42965
                     /gene="ythA"
                     /locus_tag="ACG0BW_001061"
     CDS             42840..42965
                     /gene="ythA"
                     /locus_tag="ACG0BW_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YthA"
                     /protein_id="HFK7258052.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(43018..43275)
                     /locus_tag="ACG0BW_001062"
     CDS             complement(43018..43275)
                     /locus_tag="ACG0BW_001062"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFK7258053.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            43298..43501
                     /locus_tag="ACG0BW_001063"
     CDS             43298..43501
                     /locus_tag="ACG0BW_001063"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7258054.1"
                     /translation="MDITPKYVGIASGLMNAGSAVADIISPIAFGIIIDKTGNWSLPF
                     YGSVALLVIGIFLTFFMRPDKSL"
     gene            43589..44341
                     /locus_tag="ACG0BW_001064"
                     /pseudo
     CDS             43589..44341
                     /locus_tag="ACG0BW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT        10938 a        10828 c        11109 g        11466 t
ORIGIN      
        1 agaaaaatta aatcctcagg gaaatttacg tatagcaata gaaaaatttg ggtggaataa
       61 taaacctatc actgcataaa ataatgtgat gccgggatgg tttgtatttc ccggcatctt
      121 tatagcaata tcaattattt actgagcgtc gcgaccatca ccgctttgat ggtatgcatg
      181 cggttttccg cctgatcaaa aacaatgctg gcggcagatt cgaagacctc atcagtcact
      241 tccattccgc catgtaggcc aaactcttcc gccattttct taccaagcgt cgtctggtcg
      301 tcatgaaaag cgggcaggca gtggaggaat ttgacctccg ggttaccggt caactgcatc
      361 atcttgctgt tcacctgata atcacgcagt aatgcaatcc gttctgccca tttctctttt
      421 gcttccccca tcgacaccca cacatcggta tagataaagt cagcaccttc tactcccttg
      481 gctacatctt ccgttagcgt aatattcccg ccattttgct gtgccagagc gcggcattcc
      541 gtaaccagcg cagcttccgg ccagcatgcc tgtggcgcga ccagacgcaa atccagaccg
      601 gtaagcgccg cagcttcgag catcgaattg cccatgttgt tacgtgcgtc acctgcatag
      661 accagcgtca tttcgttgaa cgctttgcca ggtaaatgct cctgcatggt gaggagatcc
      721 gccagcagct gcgtggggtg aaactcattg gtcaggccgt tccataccgg cacgccagcg
      781 tattccgcca gtgtttcaac aatctcctga ccatagccgc gatactgaat accgtcatac
      841 atgcgaccaa gcacgcgggc agtgtcttta atcgactctt tatgaccaat ctggctgccg
      901 cttgggccga gataagtaac gcgagcaccc tggtcatatg cggcaacttc gaaagagcat
      961 cgggtacggg tcgagtcttt ttcgaagatg agcgcgatgt ttttaccagt gagtttggct
     1021 tcttctttac cgcttttctt atcggctttc agcttcgcgg ctaactgcaa caggctgttg
     1081 agttcagctg gcgtgaaatc gagtaatttc aggaaatgct tatgataaaa cccggacata
     1141 gatccctcct gtggctatcg cctcaatgaa ttaaaattca atttatatgg atgattattc
     1201 atttgcaagt ctaaagcata aatctttgtc acaaaggtgg aggcaatgtc agtggtgtgt
     1261 gacaataaga gtatcggcag gacattaaga ggaatgagcc atggcaaacc cggaacaact
     1321 ggaagaacag cgtgaagaaa cacgtttgat tattgaagaa ttactggaag atggcagcga
     1381 tccggacgcg ctgtacacca tcgaacacca tctttccgca gacgatctgg aaaccctgga
     1441 aaaagcagca gttgaagcgt ttaaactcgg ttacgaagtg accgatccag aagagctgga
     1501 agttgaagat ggtgatatcg tgatttgctg cgacatcctc agcgagtgcg cgttgaatgc
     1561 cgatctgatc gatgcccagg ttgaacaact gatgacgctg gcggagaaat ttgacgttga
     1621 gtacgatggt tggggcactt actttgaaga tcctaacggc gaagacggcg acgatgaaga
     1681 ttttgtcgac gaagacgatg acggggttcg ccactaatta attgattaaa atgcccgata
     1741 gcacacacta gcgggcattt ttttagagtt cgcgtagcat ccgcacttca caatcgacat
     1801 ggcccgtgca gccaagcgca tagtcgatat gctcaaagcc caaatgctca taaagtgcaa
     1861 tggcttcctt taaaaaagcg gtcgtttcca gataacagcg tttgaagccc atctctcgtg
     1921 cctgctccat cgccattaag gccagttttt tcgccagccc tttaccgcga atagccggaa
     1981 gaaaatacat cttttgcagt tcgcaaatgt ccgattcact acctgctaat ggcgcaatcc
     2041 caccgccgcc gaccacttca ccatcgaact caaccaccca atatgcatgg ccaggctgac
     2101 tatacacttg atacaactcg tcaaggttcg gatcggcgac ggtgtagcct ttatcagcgg
     2161 taagaccgta ttcggcggat acctgacgaa tgacgcgggc gatagcggga ttatcctgct
     2221 gcgtcaggcg gcgcattaca ggtgattgcg gcgcaatgtt attcatagtt taacccaata
     2281 aaaataaagt aattatacat ttgtttaata ccactctctt cttacgtgcg caagcgactt
     2341 ataaagtaat aaagtttttg ccagcctatt actgataaga gtattgtaac gtggcagaac
     2401 attttactgt ctgtatatat aaattatatc aatggatttt ttaattcagg gaatttttat
     2461 ggctcaagtt attaatgaaa tggatgttcc gtcccattcg tttgtttttc atggcacagg
     2521 tgagagatat tttcttattt gtgtggtgaa tgtgttgtta acgattataa cgctaggtat
     2581 ctatttgcca tgggcattaa tgaaatgtaa gcgttatctc tatgctaata tggaagttaa
     2641 cggacaacga ttttcttatg gaattaccgg tgggaatgtt tttgttagtt gtcttgtttt
     2701 tgtttttttc tatttcgcaa tcttaatgac agtgtcagca gatatgccgc ttgttggctg
     2761 tgttttgact ttgtcactgt tggttttgct tatatttatg gcagcaaaag gactgcgtta
     2821 tcaggccttg atgaccagtc tcaacggcgt aagatttagt tttaattgct ctttgaaagg
     2881 gttctggtgg gtaacctttt tcttgccgat tttaatggcc attgggatgg gagctgtttt
     2941 ctttatctcg acaaagatgc tacatgccaa tagttcaagt agtgttatta tatctgtggt
     3001 tctgatggca atagttggta ttgtttccat tggtattttt aatggtactt tatatagcct
     3061 ggtaatgagt tttctctgga gcaataccag tttcggtata catcgtttca aggtgaaatt
     3121 agatactacg tattgtataa aatatgccat tctcgcattt ttagctttat taccttttct
     3181 cgctgttgct ggttatatta tcttcgatca aatattaaat gcgtatgata gttctgtgta
     3241 tgcaaatgat gatattgaga atttacagca atttatggaa atgcaacgta aaatgataat
     3301 cgcgcagtta atctattatt ttgggattgc tgttagcacc agttatttaa cggtgtcgtt
     3361 gcgaaatcat tttatgagca acctgtcact gaatgatggg cgtattcgtt ttcgctcaac
     3421 tttaacgtac cacggtatgc tttatcgcat gtgtgcgttg gtggtgatat ccgggattac
     3481 gggcggtctg gcttatccac tgctgaaaat atggatgatt gactggcagg caaaaaatac
     3541 gtatttgctg ggcgatttgg atgaccttcc tttaatcaat aaagaagaac aaccagataa
     3601 aggcttctta gccaggattt cacggggaat tatgccttct ttaccatttc tgtaagagat
     3661 aaaaaaggcc ggagcatgct acggccttcg ttttcatcgc tgtgatttga ttacagcgcg
     3721 gcgataacag cctgctgttc aatcagcttc gctttcgctt ctgcatagcc ttccagcttt
     3781 tcacgctctt tcgcgatgac cgcttccggt gcgcgggcga caaagccttc attcgccagc
     3841 ttattctcga tgcggctgat ttcgccttcg atcttcgcca cttctttcgc cagacgcgcc
     3901 agctcatctt ctttgttgat gaggccagcc atcgggatca gcagctctgc gccgtcgatg
     3961 atcttcgtaa cggaaaccgg acctttgtta tcggcaggca gcacggtgat gctttccaga
     4021 cgcgccaggg tttgcaggaa gccacggttt tcatttacgc gacgttctgc atccgcgctg
     4081 caaccacgca gcagcagctc cagcggtttg cccggtgcga tgttcatttc tgcacggatg
     4141 ttacgtaccg caacgatcgc ctgcttcaac cactctgtgt cggccagtgc ggcttcatca
     4201 acctgagatg catcgtactg cgggaacggc tgcagcatga tggtgtcggc agtgataccg
     4261 caaagtactt tcacacgctg ccagatggtt tcggtgatga acggaatgat cggatgcgcg
     4321 aggcgcagca gaccttccag cacggtcacc agcgtatggc gagtaccgcg cagttcggct
     4381 tcggtgccac cgttcattac cggcttagtc agctccagat accagtcaca gaactggttc
     4441 caggtgaact catacagaat gcctgcggcg atatcgaagc ggaagctgtc cagcgcttcg
     4501 cggtacgctt tgatggtctg gttgaactct gccagaatcc agcggtccgc cagcgacagc
     4561 gtcatttcac caccgttgaa gccgcaatcc tgaccttctg tgttcatcag cacaaagcgg
     4621 ctggcgttcc acagcttgtt acagaagtta cggtaacctt ccagacgctt catatcccag
     4681 ttgatgtcac gaccagtaga agccagcgcc gccagggtga agcgcagcgc atcagtaccg
     4741 tgcggctcaa tgccgttcgg gaactgcttc tcggtgcgct tacggatttt gtccgccagt
     4801 tgcggctgca tcatattgcc ggtacgtttt tccagcagtt ctggcagcga aatgccgtca
     4861 accatatcca gcgggtcgat aacgttaccc ttggatttgg acatcttctg gccttcgtca
     4921 tcacgaatca ggccggtcat gtaaacggtg tggaacggca cctgcggttt gccattttca
     4981 tctttgatga agtgcatggt catcatgatc atgcgggcaa tccagaagaa gatgatgtcg
     5041 aaaccagata ccatcacgct ggttgggtgg aactgacgca gggcgtcggt gttttccggc
     5101 cagcctaggg tagagaaggt ccacagcgca gaagagaacc aggtgtcgag aacgtcttcg
     5161 tcctgacgca gggcaacgtc ggcaccgagg ttattttctt tacgcacttc gtcttcgttg
     5221 cggccaacat aaacgttacc cgcttcgtcg taccatgccg ggattcggtg accccaccac
     5281 agctgacgag agatacacca gtcctgaata tcgcgcatcc aggagaagta catgttttcg
     5341 tactgcttcg gtacgaactg aatgtcgcca ttctcaaccg cttcaactgc cggtttcgcc
     5401 aggacatcgg cacgcacgta ccactggtcg gtcagcatcg gttcgataac tacgccgcca
     5461 cggtcgccgt aaggaacggt caggtcgtgc ggtttaattt cttccagcag gccgagtgcg
     5521 tcaaccgcag caacgactgc tttacgtgca gcaaaacgct cgagtttctg gaactctgcc
     5581 gggatttcgc tggaataaac gtcagattcg ttacctttgg tatcgaacac ctgggcgctt
     5641 tcacggatat cgccgtcaaa ggtcaggatg ttgatcatcg gcagcgcgtg acgtttaccc
     5701 acttcatagt cgttaaagtc gtgcgccgga gtgattttca cgcagccggt gcctttttcc
     5761 atgtcggcgt gttcgtcgcc aacgatcgga atacgacggt taaccagcgg cagaatgaca
     5821 tatttgccaa tcagatcttt gtaacgcgga tcttccgggt taacggctac gccagtatcg
     5881 cccagcaggg tttccggacg ggtagtcgca accaccagat aatctttacc gtctgcggtt
     5941 ttcgcgccgt cagccagcgg atagcggatg tgccacatcg aaccttttga ttcgcggttt
     6001 tccacttcca ggtcagagat agcggtgcgc agtttcgggt cccagtttac caggcgtttg
     6061 ccacggtaaa tcaggtcttc tttatacaga cgaacgaaaa cttctttcac cgcattggac
     6121 aggccttcgt ccatggtgaa gcgttcacgc tcccagtcga cggagttgcc gagacggcgc
     6181 atctgacggg taatggtgcc gccggactcc gctttccatt cccagatttt gtcgatgaaa
     6241 gcttcgcggc cgtagtcgtg acgggtttta ccttcttctg cggcaatctt gcgctcaacg
     6301 accatctggg tagcgatccc ggcgtggtca gtaccgacct gccacagggt gtttttgccc
     6361 tgcatgcgct gatagcggat catggtatcc atgatggttt gctggaaggc gtgacccata
     6421 tgcaaactgc cggtgacgtt cggcggcggg atcatgatgc agaaactttc ctggctttca
     6481 tcgccattag gcttaaagta gccctgcttt tcccagtgct cgtaaagcgg ctgttcgata
     6541 tcttgtgggt tatatgtctt ttccattatt tccaggttgc cgtattcagg ttgaaaccag
     6601 ccacgcggta ggctttatag cgttcgcgcg ccagttgttt cagagaatct tcataaggaa
     6661 cgaagtctac cacttctgtg aaagcggtgg caaaatctgc aaagcttgtt cgcagactaa
     6721 tcaatatatc gcgcgggctg ctgctgcgtt tttgcggcca ggcgatctcc accggcgcac
     6781 cgccgcgcgg tccttctccc gctaaattat gcggaacaaa gctttctgcc ggacgcgccc
     6841 acagggcttc atccagccgg taagcctgct tttcatcttc acaggcgatg agcacgcgct
     6901 taccgctgcg ccaacgttct gcggcaattt cacacaccag ttgctcaacg gcgcttaagc
     6961 catcgacggt ggtgtcattg tccagaaggt agaacgtcgc gtttttcata tatggggctt
     7021 cttgtggtgg atttaaatac gcatcaggta tacaccgcag cgcctgatgc gacgctgttg
     7081 tgtcttatca ggcctacgag ttcagtgctg tgtgtaggtc ggataaggcg cttacgccgc
     7141 atccgacaat aacagcattg cctgatgcga cgctgccgcg tcttatcagg cctacgagtt
     7201 cggtgctgtg taggtcggat aaggcgttca tgccgcatcc gacaataaca gcattgcctg
     7261 atgcgacgct tgcgcgtctt atcaggccta cgagttcagt gctgtgtagg tcggataagg
     7321 cgttcacgcc gcatccggca ataacagcct tgcctgacgc aattactctt cgccgttaaa
     7381 cccagcgcgg ttcaacagga actgtgccag caacgctacc ggacgaccgg ttgcgccttt
     7441 tgctttacca gaacgccagg cggttccggc gatatccagg tgcgcccagt tgtacttacg
     7501 ggtaaagcgc gacaggaagc aacctgcggt aatcgcccca ccaggacgac cgccaatgtt
     7561 cgccatatcg gcaaaattgg actccagctg ttcctgatac tcgtcaccca gcggtaagcg
     7621 ccatgcgcgg tcaccggatt gttcagacgc ggcaatcagt tcatgggcca gcggattatg
     7681 gttcgccatc agaccggtaa tgtgatgacc cagcgcgatc acgcaggcac cggtcagcgt
     7741 cgccacgtca atcaccgctt ccggctcaaa acgctcaacg taagttaaca cgtcgcacag
     7801 taccaggcgg ccttcagcgt cagtattcag cacttcaacg gtttgaccgg acatggtggt
     7861 taacacatcg cccggacgat aggctcgtcc gccaggcatg ttttcgcagc ctgccaacac
     7921 gccgataacg ttaatcggca gttgtagctc cgcgaccata cgcatcacac cgtaaaccgc
     7981 tgccgcaccg cacatatcgt acttcatctc atccatgcct tctgaaggct tgatcgagat
     8041 accgccggag tcgaaggtta aacctttacc caccagcacg attgggcgtg catcttccga
     8101 cgcgttgcct ttgtactcaa tcaccgacat cagcgattcg ttctgcgaac cctgaccgac
     8161 cgccagatag gaatgcatcc ccagctcttt catctgctgt tcgccgataa cgcgggtgat
     8221 aacattcttg ctgtagctgt cagccagctg gcgcgcttgt gaagcgaggt aagcggcgtt
     8281 acagatattc ggcggcatat tgccaagatc ctttgctgct ttaatcccgg cggcaatcgc
     8341 cagaccgtgc tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg gcacgttgaa
     8401 caccatttta cgcagcggac gacgcggttc gctcttgttc gttttcagct gatcgaaact
     8461 gtagagcgtt tcttttgccg tctcgacagc ctgacgcact ttccagtagt tgttacggcc
     8521 tttaacgtgc agttcagtca gaaagcagac cgcttccatt gagccagtat cattcagcgt
     8581 attaatggtt ttctgaataa cctgcttgta ctgacgctca tccagctcac gttctttgcc
     8641 gcaaccaata aggagaattc gctcggaaag tacattcgga acatggtgca gcaacaatgt
     8701 ctgccccggt tttccttcca gttcgccccg acgtagcagg gcgctgatgt acccatcgct
     8761 gattttatcg agctgttctg caatcggaga aaggcgacgt ggttcgaaga cgcccacgac
     8821 gatgcaggca ctccgctgtt tctccgggct accgcttttt acactaaact ccatgcacta
     8881 cgctcctgaa tcttaaagac aacggcggtg gctacagata gaattgcaag ctttcgtaac
     8941 tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg ttattacggc attggcacgt
     9001 cagaacaaaa tctgagaggt gaatccgttg agtataatta tcttagcgac gatttcgacg
     9061 actcaagaga ataaatgacg tttaagccat gaaacaagct aaaatcctgc aaaagacgag
     9121 tttttacggg cgtatttaaa gtgataatca taagatatct ggtgcgggag acgctcaaaa
     9181 gccagctggc gatactcttc atcttgcttt tgatcttctt ctgtcaaaag ttagtgagga
     9241 tcctcggcgc agcggttgac ggcgatattc cggcgaatct ggtgctctcc cttctcgggt
     9301 tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag cctgttcctc gggctgctga
     9361 tgacgctggg caaactgtat accgaaagtg aaattacggt aatgcatgcc tgcggcctga
     9421 gcaaagcggt tctggtgaaa gcggcaatga tccttgcggt attcacggca atcgtagcgg
     9481 cggttaacgt gatgtgggcg ggaccgtggt catcgcgtca tcaggatgaa gtgttagcag
     9541 aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg gcaattccag caagcgacta
     9601 atggcagctc ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa gatgtgttcc
     9661 tcgcgcaaat tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc gattccggac
     9721 atttaaccca gctgcgcgac ggctcccagg tcgtcactct caaccaggga acgcgcttcg
     9781 aaggcactgc attgttacgt gatttccgca ttacggactt ccaggattat caggcgatca
     9841 ttggtcacca ggcggtggcg ctcgacccga acgataccga ccagatggac atgcgcacat
     9901 tgtggaacac tgacaccgat cgtgctcgcg cagaactgaa ctggcgtatc acgttggtat
     9961 tcaccgtgtt tatgatggca cttatggtcg taccgctgag cgtggttaac ccacgtcagg
    10021 gacgcgtact gtcgatgctg ccagccatgc tgctgtatct acttttcttc ctgatccaga
    10081 cctccctgaa atcgaacggc ggtaaaggta agctggaccc gacgctgtgg atgtggaccg
    10141 ttaacctgat ttatctggct ttagcgattg ttctcaacct ttgggacacc gtgccggtcc
    10201 gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt ttggcgtact
    10261 tgaccgctat atcggtaaaa ctattttcac caccatcatg atgacactgt tcatgctggt
    10321 gtcgctgtcg ggcattatca agtttgtcga tcagctgaaa aaagccgggc aggggagtta
    10381 cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg tgcagatctt
    10441 cttcccgatg gcggctctgc ttggggcgtt gcttggtctt gggatgctgg cgcagcgcag
    10501 cgaactggtg gtgatgcagg cttctggttt tacccgtatg caggtggcgc tgtcggtgat
    10561 gaaaaccgcc attccgctgg tcttgctgac gatggcgatt ggcgaatggg tcgcgccgca
    10621 gggcgagcag atggcgcgta actaccgtgc gcaggcgatg tacggcggct cgttgctctc
    10681 tacccagcaa ggcttatggg cgaaagatgg caacaacttc gtctacattg agcgggttaa
    10741 aggtgacgaa gagttaggtg gcatcagcat ttatgccttt aacgagaatc gtcgtctgca
    10801 atccgtacgc tatgccgcta ctgcgaagtt tgacccggaa cataaagtct ggcgtctgtc
    10861 gcaggttgat gaatctgatc tgaccaatcc gaaacagatt accggttcgc agacggtgag
    10921 cggcacctgg aaaaccaacc tcacgccgga caaactgggc gtggtggcgc tggacccgga
    10981 tgcactctct atcagcggtt tgcacaacta tgtgaagtat ctgaagtcga gcggtcagga
    11041 tgccggacgt tatcagctca acatgtggag caaaatcttc cagccgctat ctgtggcggt
    11101 gatgatgctg atggcgctgt cgttcatctt tggcccactg cgtagcgtac cgatgggcgt
    11161 gcgtgtggtc accggtatca gtttcggttt tgtcttctac gtactggacc agatcttcgg
    11221 cccgctgacg ttggtttatg gcatcccgcc gatcatcggc gcactgttgc caagcgccag
    11281 cttcttctta atcagcctgt ggctgttaat gagaaaatcg taaccgaatg aacgaataaa
    11341 gggagcgaaa gctcccttta ttattgttag caaagtgtgc ttcgttcatt cctgaaaaat
    11401 aattaaaatt caatatattg cagtggggtg ggcatgataa gacgcgccag cgtcgcatca
    11461 ggcaaagaca aatctcgcct gacttacctt cttctccccc ccagcaaact ccccaacatc
    11521 ccgcgtacaa tctgattcgt cacttggcga gcggcgcttt tggccattgt ttgcaccaca
    11581 ccatctttct tcccgccgcg tggtccggta gtgccgaaca aaatatcctt caatccacca
    11641 agaatgccgt catccaccgc tacctctttc cctttcgcgg gaggattatt ttgctgctcg
    11701 gtactggcct gaaagccttt ttgcaacatc tcataggcgg attcccggtc cacctcatcc
    11761 tcatatttgc catacaccgg agagtgatta atcaagccat tacgctcatc ttccgtcacc
    11821 ggccccatcc gcgaacaagg cgcgatcacc atcgcacgct ccaccacaga agggcttcct
    11881 ttcgcatcca gaaaagagat caacgcctcg ccggtgccca gttcctgaat cgccttttcg
    11941 gtatcaaacg ccggattggc ccgcatggtt tgcgccgctg ccttcactgc tttctgatct
    12001 tttggcgtaa aagcccgcaa agcatgttga acgcgattac cgagctgccc gagcacatta
    12061 tccggaatat cagacgggtt ttgcgaaacg aaccagacgc ccacgccttt tgagcgaata
    12121 agccttatca cctgctcaat cttatccagc agtacctgcg gggcatcgtt aaacagcaga
    12181 tgtgcctcgt cgaagaaaaa caccagtttt ggtttctcca gatcgcccgc ttccggcaat
    12241 tgttcataca actctgaaag catccacagc aggctggcgg cgtacagttt cggcatctga
    12301 taaagtttct cggcgctgag gatattgata acgcctttac cgttggcatc ggtgcgcatc
    12361 cagtctttga tatccagcat cggctcacca aagaagtgtg ctgcgccttg ctgttccagc
    12421 gacaacagcc cgcgctggat ggcaccaacc gatgcgctac tgatattacc gtactgattc
    12481 tggaaggatt tggcgttatc gccgatgtac tgggtaattg cccgcagatc tttaaagtcg
    12541 agcagtaaca gcccctgatc gtcagcaatg cggaagatga tattcagcac gccagattgc
    12601 acatcgttga gattcaacag tcgtgccagc aacagcggcc ccagatccga aaccgtcgcc
    12661 cgcaccggat ggcctttctc gccaaagata tcccacacca ccaccggatt ggcatgcggt
    12721 tgccagtcat tgacgccgat atttttaagc cttgcgagca gtttttccga caccgttcct
    12781 gcctgcgcga cgccggtcag atcgcctttc acatcagcca taaacaccgg cacgccgatt
    12841 tccgacaatg actccgccag tttttgcagc gtaacggttt tacccgtccc cgttgcgccg
    12901 gtaatcagcc catgacggtt agccattccc ggcagtaaaa acagttctgt gtccggcgtg
    12961 cgggcaatta acaggggttc actcatgaga tatcctccag tttcctgcct ggagtatagg
    13021 caacgtccgg caaagagaat acgtgtaatg aagagaaaat aacctaaagc gtgttgccgt
    13081 gataaatctg atacccgagg tcaacagtgt tgtggttgtg atcgttattt ttaattttgc
    13141 tcagcaacat ttgggcggcc atccgaccaa tatcaaaacg gggagtaatt acgctggcga
    13201 ggcttgggat catttgcctg ccaatctcca gcccatgaaa acccgcaatg gaaatttgtt
    13261 ctggcacggc gaggtttcgc tcgcggcaca gcagaagcgc acccatcgca atatcgtcat
    13321 tggtgcaaaa tacaccgtct aaatccgggt ttgcgctaag ggcatcacgc attaactgca
    13381 ttcctaaatg aatggatgag atggcgcgtg gattcatgcg taatggggaa agattatgca
    13441 gcatcatcgc atcgcaatac ccctgataac gctgttcatc gcgggtgtca tctttggaac
    13501 cgagatacag aattttgtgc ctgactcgct tctccagcat ggtacacacc atgtcaaaag
    13561 cggcctgccg attatcaaaa ccgacctcca tatccagccg ttctccctgt acatccatca
    13621 attccacgac cggaatggtg gctgaacgca gaaatttcac cgtcctgatg gtgtgatatt
    13681 tttccgaaag tataatcccg tcaatgttat aagagagcag gttaatcacc gactcttctt
    13741 cggaatcgcg atcgtaattg taattagcaa taagggtctg ataattatgc tcagaggtga
    13801 cggattctat tcccgcgaga atgtcagcga agagttgatt ttgaaatgac ggaatcaaga
    13861 taccgagggt ataactttgt gcattcaaca acatgccggg agcgcgatta gggatataat
    13921 tgatctcttc cataattttg gcgatgcgct cgcctgtttc ctttgccacc tttttcggcg
    13981 agcggatata acgactcacg gtcatttttg ttacgccagc cagcgtagcg atatcctgta
    14041 aagaaattct gtgattgcgc ataacttctc ccaacgtcgg gggaatcttc aatcttcccc
    14101 cgaaactaca tcacaatatt ttattctttc agtgcaatac ggcgttaatg gctaacacgc
    14161 ccagcaaacc cataatagaa ataagggttt ccatcaccgt ccaggtacgc aacgtttcac
    14221 caaccgtcag attaaaatac cctttaaata gccagaagcc agggtcgttt acgtgtgacg
    14281 cgatcacgct gcccgcaccg gttgccagta ccattaaagc gggatcggca tgggtaacgt
    14341 tgataatcgg caacaccaca cccgcggtgg taatggccgc gacggtggca gagcccagag
    14401 cgatacgcaa cagcgccgca acagtccagc acatcaataa cggcgaaagt gtagttccgg
    14461 tcattaagtg tgaaatatag tgcccgacac cgctatctac taatacctgc ttaaacgcgc
    14521 cgccgccagc gataataaac acaatcatcg cgatagcgcc tatagaatcc ccaatgatat
    14581 ccatgatttg ctcgatggtg cgtccattac gtcggcccag tgtgaaaatc gcaataacaa
    14641 tggcaataaa cagcgcaacg gcagggttac cgacaaattc aaagaagagg cgcacggtgt
    14701 tagttttcgg taacgtaatt tcacaaacgg cggcgatagc catcaggatg accgggatca
    14761 cggcagcgaa aatactgttc cagaaggagg gcatctcctc ttcgctaaac agatgaggat
    14821 taaataagcc ttccggtggt gctttctcaa agcgagttag cagtttagaa aacagcggtc
    14881 ctgcgacaat aactgtcgga atggtaatga taaatccata cagtaaagtc gttccgagat
    14941 tagcctcaaa gatagtcgcg atggcagtag gccctggatg tggcggcaga aaacagtggg
    15001 ttacagagag cgctgctacc attggtacgc caacatacaa caggggtaat cctgatgatg
    15061 ctacgatggt aaataccaac ggcaacagca ggacaaaacc cacttcaaaa aacatggcga
    15121 ggcccacaac cagaccggtg atcactagcg cccattgcac gcgtttttta ccaaaagtag
    15181 caatcagcgt agtggcgata cgttgtgccg cacccgtatc agaaatcagc ttgcctaaca
    15241 tggccccgaa accgaggatc attgccagcc cgccgagcgt gctgccgata ccattttgta
    15301 tagagtgcag gacggcctgt gcatccatcc cttcggcaaa tccgacgacg gcagctacca
    15361 gaacgagggc aataaagccg ttaactttaa agacgatcat caggataaga agcagcgcga
    15421 cgcctgccgc aataatgatt aatggcatag tctttatctc ttattagtgc cagaaaagca
    15481 gcgctttcgc gctgcacgta ggggggggct gttaaacagc cactaacatg ccgccatcaa
    15541 caaacaacag gtggccgttt acgaaatcag aggcttttga agaaaggaac accgcagcac
    15601 caatcagttc ctgcggatct ccccagcgtg cggcgggggt ccgtttgcac aaccaggcgg
    15661 tgaaggcttc gtcctcaacc agtgctttag tcatttctgt tttgaaatag cccggcgcaa
    15721 taccgttgac ctgaatattg tggcgcgcca gctcgacgca catgccgcgg gtgagcattt
    15781 ttaccgcccc tttcgatgcg gcataagggg tgatggtgtc acgtcccagt tcgctttgca
    15841 tcgagcaaat attaataact ttacctgcct tgcgttcaac catgtgacga gttaccgctt
    15901 gcgataccag gaacacggcg gtctggttta ctgcgatcac atcattccac tcttgttcag
    15961 ggaactcagt aaaaggatga cggcgctgga taccggcgtt attcaccagc acatcaatgg
    16021 ggccgatgtc cttttcgata tgttcaacgg cggcatcaat ttcatgttta tgagtaacat
    16081 taaaaggtgc ggcaacggcc tgaataccct cctggtggag tttttctaca gcaagttctg
    16141 cgcgttcggc agtaatatca ttaataatta tttgtgcgcc atatttaccc aggccggttg
    16201 ccagtaaaaa gccaatgccc tgtgctgaac cggtaatcaa gatatttttt cctgccagtg
    16261 aaaatagatc gttcattgta ttattccttt atttatctct tagaaaacaa gctggacttt
    16321 tgctgcctgg gttttatcac cggcgaaacg tagcgcctct tccaggtcag tgaagggata
    16381 ttcagcactc agtaaaggca gtggattgat aacgccattc gccagccatg acactgcggt
    16441 attaaattcg ctggtaaaac ggaaagagcc tctgagtgaa atctccttac caatcaacgt
    16501 catcattggg aattctgcca tcgcgcctcc catacctacc tgcaccatta cgccgcgtgc
    16561 acgagtgacc tccagacagg tattcactga tgaaggatga ccggacactt caaagctgac
    16621 atcgaaatag cctttttccg ctttccagtg atccatgtcg tcgttttgtg ggtttacgag
    16681 cacatccgcc cccatctctt tgcccagcga aagggaacgg ggactcacat cagcacagac
    16741 aatttccgcg gcccccagtg ttttcactgc actgacaatc aggcagccaa tgggtccaac
    16801 accggaaata aatactcgct tgccctgtaa ctcgccggcc tgatgtgcgg catgaatcgc
    16861 gacggctaaa ggttcggcaa aagccataac cttttcgtca gctttggccg gataagggac
    16921 acattgcgac gtttcgacca ttttataacg ggtaaaacca ccatcaacat gagggaaata
    16981 catggcactg ccaaaaaaac gcatatctgt acactgattc tcgttatgtt caatgcagta
    17041 tttgcagtga ccgcacggtt tagacggatt aatggctacc gtttgccctt catgtaattc
    17101 tgatgagtcg ctatgaataa ctttaccgat aacttcatga cctaacacca tcggtgcctt
    17161 tatcatgaaa ttacctactt ttccttcctg ataataatgt aaatcggaac cgcaaattcc
    17221 acctcgggtt atttgtacta atgttccatt attattccaa tctatcgtct gctcggtaac
    17281 ggcaacagtt ttcttgcccg caacaacgca ggactgtgtt ttcacttgca taaattcctc
    17341 actggtcagg tagacacctc ggaagcattt aagcggtttt aactgtcatt atttgtgatg
    17401 aagatcacgt cagaaaattg ttacattact atgttacgca taacgtgatg tgccttgtaa
    17461 ttcttatcag tagaaataaa aaaacgtgaa attattatgc cgccaggcgt agtatcgcag
    17521 caggtaagat gattcaggag attttaaatg gcgggtgaaa gctttatttt gatgggcgtt
    17581 tcagggagtg gtaaaacatt aattggtagc aaggttgccg cgttattatc tgctaaattt
    17641 attgatggtg acgatcttca tccagccaaa aatatagata aaatgtcgca gggtattcca
    17701 ttatctgatg aagatcgact tccctggctg gaacgcttaa atgatgcttc atacagtctt
    17761 tataaaaaga atgaaacagg atttattgtc tgttcatcat taaaaaaaca gtatcgtgat
    17821 attttacgca agggtagccc ccatgttcat ttcctctggt tagatggcga ctatgaaact
    17881 attctcgcgc gaatgcagcg tcgggctggg cattttatgc cggtagcgtt actaaaaagt
    17941 cagtttgagg cactggagcg tccacaagca gatgaacagg atattgtgcg cattgatatc
    18001 aaccatgata ttgccaatgt caccgaacaa tgtcggcagg ctgtgctggc gatacgacaa
    18061 aacagaatat gtgcgaaaga gggcagcgcc tcagatcagc gctgcgaatg attttcaata
    18121 atcggctttc aacaccacgc ggtaacgcgc cttaccgtcg cgcacatgct ggatggcgtc
    18181 gttaattttc gacatcggga acagttcggt ggtcggcgca accttgctgc gggcggcaaa
    18241 acgcatcagc ttacgcagct cataaggcgt gccggtagca gaaccagaga cgctgcgatc
    18301 gcccgcaatt aacgtaaagg ccggaacaga cagcggcgtg agaaccgcac cgaccgtatg
    18361 gaaattaccg ccataggtca gcgcctcaaa atagggctgc cagtcgaggc tgacgttgac
    18421 ggtgttgata atgagatcaa actgccccgc cagtgctttc agtgcctgcg gatcgcggct
    18481 attcaccact ttatcggcac ccatcgccag cacttcctgc tctttcgccg gattagaact
    18541 aaaggctgtc acctcgcatc ccattgcgtg cagaagtttt atagcgatat gccccagccc
    18601 gccaatacca attaccccaa cgcggctggt agcagtgata tggtgcatca acagtggttt
    18661 aaagaccgtg ataccgccgc acaacagcgg cccggcggac tcgatatcaa tattttctgg
    18721 cagtggaatc acccattgcc agtccgcacg caacttctcg gcaaagccac cgcgattcat
    18781 aatcgtcggc accgcacctt gctcgcagtt gatctgatta ccgctaatac aggcgtcgca
    18841 gtgaccacag ctacgcgccg tccagccaat cccgacacgc tgaccgacct gcaaaccttt
    18901 atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc acctcatgcc cggcaaccag
    18961 cggatattgt gaaaatcccc attcgttatc gatcatcgac agatcggaat ggcagatccc
    19021 gcagtaatcc acctgcactt caacatcttg tggcctcagc tcaccgggat cgtactcata
    19081 aacttccagt tcgccgcccg cttcttttgc ggcatagctt tttatcatcg acattttttg
    19141 gtccttctct ggtgttgttt gggaagtgta gagcatggca gggcggggtg cctggagtgt
    19201 gacaaaggtt acacatcgct gtatgcaatg ctgaaaattt cagcacttag cgaggtgcga
    19261 gcaagctggc gcttgcatgg tggcgtgcga caggtataat ccacaacgtt ttccgcatac
    19321 ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg attcaaaatc aaccgtagaa
    19381 atacgtgccg gttcgagtcc ggccttcggc accaagtcac taaattcctt acaatacagc
    19441 tagttagaca ataatatctg tctcttttgc ctgtaagaaa tgctctgttt gtcccttgaa
    19501 aaagccgctg tggttgatag tggtaattgg gatgttaact tatagcggcg tgtagttcca
    19561 agcgtcaaac acgtactgac tcaacttgaa agctatgacg tagccaccgt ctctaaacaa
    19621 tcccaaaaac ttttagtgaa gcgcatttta gcccgatgcc cccgttctgt cgccgtcgcg
    19681 agacgccgca ggggataaac aggtttatgg tggtcacggt acttgcagac catatgcaag
    19741 ctctgccagc ggttcggcct cgctcccctt gggtacatgg tcatcctgat aagcctcact
    19801 aactgacata accctgatga agtcgccaga ggctccgctt gttgtcttac caccaacggg
    19861 gctaatggtt tgtaatggtt gaatgtatgg aatcatgtat gtggtagctt tggtacactg
    19921 catgaaaact ggtactgctg agggaatacg atgcgcacac aggaagggct taaagccatt
    19981 tacgaagcca gatggtcaag gtacgagaag cacctgcatg ctggtatgga taccacctca
    20041 atcatactaa aagggcatct tttcgttgag gaattacttt ttgaaatttt gaaattgcat
    20101 tgtcgggata gcggcccgat tgagaatatt aagctcggct ttagccataa gctaaaccta
    20161 gtgcatgcta tgtttggatc tcatttaccg ggcatggaat tccccaaaaa tgtttgggct
    20221 gctttggata aattgaacaa gttgcggaat gcacttgcac atagcattga ctctccaaga
    20281 gctgccgaag tcttcaagtc ctttgtttca tcgtacagcc aattggttgg cagagaggta
    20341 caggttcgca tcactgatga aattccgggg atagaccgca agctcggact ggagttgatt
    20401 ggtatcatta tgtatttgct cggattctta gggtgcttgc atggtatagc ctatctcaac
    20461 ccaccactca tgtacggcag tgaaatccca gttacgatac ccaacaagat tgagcacgag
    20521 tgacagtaag ctgacaaagc aagacattgc ttgccgcttg ggttggacat aaaatatcca
    20581 ttaaattaat agtgggtatt atgcatggaa tacgtggcct ttggtgatga gagtggtacg
    20641 acaggaagtg accgctgtta cggcattggt ttgctgtgtg ttcgtaaaga cacactgcat
    20701 atctttaatg agcgcgttca gaagctgaaa gacaagtacg gtattgtcgg tgagttgaag
    20761 tggtcgaaga ttaagaacag cgcagggcag gcaaacatct gcattgagct gctggcaatg
    20821 gtgctcaaga actcatgctg ctttcactcg attatcgttg tgaagaatat gtacaacaac
    20881 tggcagacgg acagggagct ggctttctac caaacgtaca caatgctcgt taagaacgtt
    20941 gctaagcaga ttaagagtga cgttgaagtc atcattgacc agaagattga taagtacaaa
    21001 aagaatgatg aggtgacggg tattgttgct aaccatatgc tggctagggc agggatgaag
    21061 aagctggtta agtctgtgac gatgcacgac tctaagcacc atctgggatt gcaggttgtg
    21121 gacatcctga ctggggcagt gaactccggg tacttgaagt acctgaatcc aaggctagaa
    21181 ctgtcagtgg ctaaagagct ggcctttgag cggatggctg ccctgcttgg ttgggatgtg
    21241 tttcattacg atacgttccc gaataaggat ttcaatattt ggcacttccc gcaagagatg
    21301 cgagctatgc cgggttccaa gagtattcgt ccagattaca gcgttccgct ggttaagcgg
    21361 gaagagctgg cctgacctac ccttatcgca agctagatct gagtggaaaa ttgatgagtt
    21421 acgatgacct agatcccgca acaaagcgag tgttgcaaca agctgagtat atgagaagca
    21481 atgaagctaa gttggcgcaa atagcatgta taaagcaact cgtggcatat accaattggt
    21541 gtgctgagcg aggcgatttt ggcgatccga acccagctac aaaagaagac tcgctaaagc
    21601 ttctgcatgt acgccaaatg cgtattgggt acgacacgag acaagtgctg gagtgtggct
    21661 ttgaggggct gtacgaacat atcgataatg cccttgaaaa tgcgctagcg tggagagact
    21721 atcgggttaa agagtgggcg gctgaatctg atattgctga actaaatgct ttatgggaat
    21781 ggttccgaga gagactgccc gctgactacg tatcaccgta ttagaaatag aatgcagcct
    21841 tatcccaaaa gatgttagca atccccaccg caagcggctc tcaggggctt gtgcgcacga
    21901 agtaatgcgg ttgtactggg tatgtgcgca cagccctcta caagccgcat tctaccgtta
    21961 gcctatagct gaatccccgg cttgaaaaat tgcatgttta tggttgcccc tccctcgacg
    22021 gttaaggctt caacttcccc cgtgcgcctc tcccagccga aggctgggcg ggctgtatat
    22081 cgatttgcct tcctctgtgg cgtttatgac cctgtttggc gttctgagcg tgaagctgtg
    22141 ggttagggct actcttctct ttaaacgcta aatctctcct gaatccctac tgttaaagca
    22201 ctttgcgttg atgctgggtg ctgctcttgc ctgcgcttgt atctgtctcg tttgggtata
    22261 cctgataaag acacagacac actgtctcat ttgactatcc ataaaactct attgactaca
    22321 cattattgat gtgatctctt ataggtcttg ctgtgatatt tcattgagac ttagggggga
    22381 agcatggcac acaatacgct tatctattgc cgcgccagta cagcagagca acacgcagac
    22441 cgggcgctgg gcttgctgcg agagttcgcc agtgatcggg gctggcctgt acgggcggag
    22501 tacatcgaga acgccagcgg tgcgaggctc gctcgccctg aactgatgcg cctgctggat
    22561 gatgcgcagg ccggagaggt cttacttgtc gagagcatcg acaggctaag tcgccttacg
    22621 caaaaggaat gggcgaccct gaaacagaga attcaggata aggggctggt gatcgttgcc
    22681 gttgacctgc caacctcatg gcaactactg aacgatacag cgtcagagct tacagagggc
    22741 attctgagag ctgttaacgc aatgctgatc gatatcctcg ctacgatggc acgagtggac
    22801 tacgagacac gcagagcacg ccaagcgcag ggcatcgagc gtgccaaagc cgaggggaaa
    22861 tataacggga agtctaagga tgagagcgcc cgtgaaattg tacgcgctat gcttaccgtt
    22921 gggaataaac cagagcacat catgaaagct gccgggataa gccgggcaac gttctaccgt
    22981 atcaaacgcg agctgtccgc cagtgttaca gagtgacaac ctacagcctg attaggtgcc
    23041 agcactggcc gttacgaggc tgtgggtgat ggatgaccta cagcttttgt attgatttca
    23101 gagagttgtg ttatccttaa tgtcgtaatg ctatgattta taatgtattt tcgtattact
    23161 tgcggctctc gatgtgaaga ggtggcgggg ctttagccgt gccagacgat gaacatcgag
    23221 atcagattgt gaaagtgatt ttcactgaac aattcaaggg atggggcagg ggaagggatc
    23281 aaaggctgct ttgtccaatc actaagctag attagaagat ggtcaacacg taagtagtat
    23341 ctttaaagtt gcatgttaaa tacatgtaca aaaaagggtt ctttccctac atccccatct
    23401 cctaatcagt gtatcgcttc acgctcacca ctggctacgc ttctcgcgtc cgctagtccg
    23461 ctttcattcg ccttcggctc attcatcggc ctgcgtccgc tgctgatgct cggccctcca
    23521 cgcaaccgtg cttgccgggc ggtcaacgtg gcggggttgt gtagtgggac ggtactctgt
    23581 atcggtatta tttctgtatt caatcttatc ctgatatcgg tacggtattc ttcataacat
    23641 cttcaattca tttctgtagc attcaatcat cttccttgtt cgtttccttt caggtcttag
    23701 ccgttggtca ttggtcttac tgcctgctgt gctactgcca gctatggcac gccgtcacgg
    23761 tctactgtct gttacctgtc ctactgcgtt cactgtctgc cgtctgtcat cctgtcggat
    23821 tccatccgtt gccgttccgc gctcgcttgt ttcggctgcg ccgcgctcgc tctgcgtatc
    23881 tagcgatact tatcactata gacactttca taattatata atgattacag taacttatgt
    23941 tttctgtttt aaaagctact aataaggaga tatagagcat tacttacccg cttttgaccg
    24001 acaaaaatcg cgttgttaag tattgccagc ccaggctcaa taaggcttag cgaggtggtg
    24061 gctaaaaaat gagctaatga aggtatctac taaggagaaa gaataactcc cgaagcgcga
    24121 caaactctcc gattttgaaa gtgactatta aggcagataa agatactgac actgttgccg
    24181 acatgaaaac ctctataagg gcagagagtc tgccgcacct gtaccaccgc cccaacctga
    24241 gctaatacct acccctattt tctggagatt tcaccatgcc aacaaaccta cgccgcctgc
    24301 ttgcgcaggc tgccgacgct gacccgctca atactgcgga cgcagatgta cagcaaatga
    24361 tcgctgacgg ggttgttatt cgacgttcca agatatgccc cgtaaagcgt aagcccgtta
    24421 aggcggcata caacacaaag accgaagagt tttattacaa tgcagatggc tccgcgatga
    24481 ccttgcggga gtggcgaacg ctgcacggtg ttcaaccgaa gaaacaaaaa cagcgctctg
    24541 acgctgggtt gcggcaagtc atccagcccg gcgacgcgat tcactgccga cgcgctgaga
    24601 ttgagcaatg gctaaataaa aagcctgctc gttatgttga accactgccg ccagatttcc
    24661 ccggcgttcg cgtagacgat gccgcattga aaccgcgcga gcacgccgtg ccggaatcaa
    24721 caaccggaca gaatgctttt ctcaaagcct gccgcgagtt cctgatttgc gatgagaaga
    24781 ccatgcgccg cattaacagg ggcatgagta aactggaagc gatgcgtaac gcagatttca
    24841 aacgctacgg agactcaggg gagtttcgtc aattttggtg tggagagtcg aagcctaccg
    24901 aagatagatt taatagtaca aatcatcatt gtgacccgaa cggcttggct gcttcgccgt
    24961 ctgtgccgcc actgaatact acgccgtatg agttccgccc gctggctcgc tgtgaccgaa
    25021 acgcacgcgt tagcctgctg ccacctgatg ggctggagtg gaggccgggg caattgtctg
    25081 ttgctgatgc gatcttttcc cgtggcagtg atgtgcttgc cgttctgcct actgggtatg
    25141 gcaaaagcgc tgccttcact tacccattga tttatgaatc ccgcgctggc gttggcctgt
    25201 ctgttatcgt tgttcctacg aataccctta agcgtcagat tctccgccag ttgcgggagg
    25261 ctggcgttca cgctgtggca tttggtgaca agctgccgag gcaccgcaat gcgcaacaac
    25321 tggcggggct ggctgccggg gcgttccggg ttgcagtcgt ttcgcctgaa catctggttc
    25381 cgtcgagtca ggttctcgcg gcgctgatgg cttatggcgt tgatcgtttt attatcgatg
    25441 aggcacacac ggtcaaagac tgggatttcc gcccagctta cgacgcaatg cagcacacac
    25501 tactgaatgc ctttccttcg gctcaacgct gcgtgtttac ggctacgctg ctacgcgaaa
    25561 atgagcgcag tttaatggag tcgcttggct gccgtggcga ttacgtaaca ttccgcgctg
    25621 atgttgctcg cgagaatatc cagttgacgc cagtcagaca gtgccgcgaa cgtttccgag
    25681 cggctattgg tctggtcgag gctctgccga aagaagagaa gacgcttatt ctcgtcaatg
    25741 accttaaaac gttgcgggcg tacctgaaac tcttgggcga ccgggcgcgg ccatatcact
    25801 cgaagacgat gcacctgagc aacgaggaac gccacgcaaa cgctgagtgg tttgcctcta
    25861 cgccgggcgc gattctaatt gctactaaag ccttcgggct gggtgctgat gttcctgata
    25921 tccgtaacgt tgtcgtgaca tacgtccccg atgatttgcc tgatctcgtt cagatgattg
    25981 gacgagccgg gcgcgatggg gaacctgccc gcgcttttct gctgtggtca tcgctggcgc
    26041 tggatgttgc gcctgatgag cttgcttact tctaccgccc tgaaaactgc aactggcagt
    26101 acatcgctaa atacctctga gtcgaaaggc tctccctgat tctctggagt taatcatatg
    26161 cctgttaccg aacatgcgca gcgtcgtgct gcgcgtgcgg atgcgctcat caccgcacaa
    26221 ctagctttcg ccctgaatgg gtacaactgg gatgaactgg ccgaacctca tcgcaagacc
    26281 cttaatcgtg aggccatcat tcgcgaggta tctacaccgc tggaacgata ttgcctgctg
    26341 aatgattgcg agccgtggga gtgtctggcc gctgtactga accgcatgaa aaggccggat
    26401 ggctcgcgtc gctggaaagc catccggttg tccgaccgga ttttcaatgg ccgggtgcat
    26461 ccggtacgca agactcctga tgaagtttac gccgccattg cccgccgttt gtttctgccg
    26521 aagcccgtgc atcaagaagt gattcagcac atccgtgcgc taggcctgtt accagttgga
    26581 gataaccacc aatgacgaaa tctgaatttg aagccggagc atgggaacta aaaaccctgc
    26641 tgctgggtgc gttgacgcgc aggcttaagg aggacgaggc gggagagtca cgcatggctg
    26701 ctgccgagtt gtcatcctgc gtgaacttcc tcaagcagaa cgccgccgac tcgcctgata
    26761 caacctttaa caacgtgttc ccggaggatg acgaccaatg acccctgaac agttccggca
    26821 atggttcgcc acgctgccgg agaaatatca acgtgtagtc cgcttcatgt acgacaacgc
    26881 ccgcaagtcg ggcacacaca ccgcgtcaag ccttgcgctg attcacacgt tggttactga
    26941 ccagatcaaa tttgattcgg agtttaattt atgactttga gtattaccga tatccagcgc
    27001 attaacgcgc tggctcataa gcacctaaag aacaatgaag agattgcagc ggagacaggt
    27061 cataccctga ccgatattga tgcagtaatc cgtggcgtct atgtcgatga cttggccgaa
    27121 cattggaaag acgatgctga tattaatctg gcggagtaca acgccatccg cagccgctat
    27181 aagcccggca agctgcaccg cgatacagag tttatgattg cccgcgatat gctgattcgc
    27241 cctgagacgg tgctggctat cgcccattcc cgctttagca acgcccacac gcacccggta
    27301 cagaaaccct tgaaggtgaa gccagcgaag ccgaaagtag tgcgcaagtt agtgcctact
    27361 gatttagatg aaatccacaa gttagagcgc ttcggttggc ctgctgcgga gatcgccggg
    27421 aagctgggcg tccctgttga ggtcgtcgcg catgtgcaac gctgcctgag aaatcgttgg
    27481 tcgtacccgg atactctgga gtctgttaac tccaaattca gcgaggctga atgatggcag
    27541 agaacgaacc gctgcacccc gccgttaacc ctgatctcta cgtgaacctt gacgaacaac
    27601 gcgaaggata cgctccgatt gaatcacggg gttgggatgt gctgacgcag aacctgaccg
    27661 ggaaaggtcg aactgacccc agccaaacac ttaaccagaa tccgaaacaa cgcccgcgcg
    27721 gctccgttgg tgagcgtcgt tggcttcgta aacgtggatt actttaacga ggaattacca
    27781 tgagcaaaac tatcgtattg ctgccgcccc gcaagaatac agactgggct gcacaattaa
    27841 agctgatttc tgaatcgctt gaggtgagtc aggcagacct cgcccacgcc tatcaggttg
    27901 atcgtcggga tatgggtaaa gcgtatcacg gtgtgcgcaa actgccggaa cgttgtgtgc
    27961 ctgttcatat gctgttgtta gcgcaggtac acgaccttag agcattatca ggagagtaat
    28021 ttatgtctga ccagaatgtt aatccgtacc tttccggtgc tgatgctgca tcgcagcaat
    28081 cgccatacat ggctgcacct gttggagctg acaatggagc atcccgcttt agctctgcgc
    28141 ctgctgatgc tgatttcctc cgtacccaac ccgttaacgg caatgatggt ggacaatgga
    28201 ataatggcaa tatcgcaaat cagcaagacc agaacgctga cagctctggg gcacaagtgg
    28261 gcgacacact gcaattacag gatggcacca ctgttgagtt gactcctgaa cttcatgggc
    28321 tgatggggca gtttcaccag tctgtcgaaa gccagctagg tcagggtaca gttggcgcga
    28381 tgttgcagta cgctggcaag ctcgacgccg ggacgcaatc aacccttgaa cggatggtca
    28441 tgtctggcga tcccgcgcag atgtcgcgtg cggttcagta tctgcaagag cgtatggctg
    28501 gcggttccat taccggttac gtcccgagcg gccagactgc cgcgcaacct gtcggaatgt
    28561 cacatgctga gtttatagca gcgtatgacc agctttcgca gtgggcgcag caatcagggt
    28621 atggccccgg ccatcgccag tacgatgaac aataccgtgc tctggttgcg cagcgctccg
    28681 ctggcaaacg cgctggccgc taacgcccaa cggtggaatg cgccctgcaa gactgagtgc
    28741 gcaccaaccc ggtactacca caccaatcta tgcgcatgag tccccgcaag ccgcattctg
    28801 cgcggcatct gaaagcctat ttttaattaa ccgaggttcg taatgagcac ctgtaatttc
    28861 catgccgtgg agcgtgtcgc tgacgtgcgc ccagttttcg ccttgccgct gggtagatgc
    28921 gaccacattt accctgagcg tgtgcagcac gttttcaatc cagacgaagc ggtttatatc
    28981 gttgcccggt ctgccacatc cggcacccgc acatctggcg ttagccgtgg gctttcccct
    29041 gtgctgccgc cgaagatggc cgagcggatg cgcgaacgta aacggcacga gaagaaccga
    29101 gccagacccc gcaaggtgat cactgaggcg atacgcgctg acattatcga tcgctctgca
    29161 aaaggccaaa agaattgtta cattgcaagc aaggttggcg tgtctgaaac ctctgtgcgg
    29221 aaggtgttga acatggcgcg tatgagtgcg tactaatgca aggtagtacg ttcgccttac
    29281 ttcacgcgca ctaacccctg agaaccgcgt cagctctggc ctcacaacgc ccatttcggg
    29341 gcacctcatc cctttaatcg ataccgttct gaatcctgcc tcggcgggag gattccgcac
    29401 gtctgtgcca tgacgatggc gcgggcttca tcaacctacg taggagtttt gttatggctg
    29461 atacttcgta cagtcccgat ctgtttcgcc cacattatgg cggggtggaa tccgacaatg
    29521 atattcacat cgaggcttat gaccgggatg tccagacccg tttagagtcc aaatccgtat
    29581 ttctggccga ggggctgtct acatacaggc cgctgaaagc cagcaaccaa taccgcattg
    29641 atcgcctgag tggtgatctg gaggtgaagg gcagaaccgc agggcaatcg ctggagccta
    29701 ccgctgttaa gtcggataag ctgattatct ctgtggatac gctgagctat atccgcatcc
    29761 cgattgacta tcaggatgac tggacagcac ccacctttcg ctcagagctg agccgcaata
    29821 tcggtactgc tcacgctaaa ttctacgacg aagcgcacac catccagcta attaaagcga
    29881 ctgactttgt accaccgccg acactgcaac caacgtttaa cccgcttaca gataacgttg
    29941 tcccgctcaa tctaaccacg ggcagtgaag aggacgcggc aaatgagctg gttcacgcca
    30001 ttaaagcggc tgctaatcgc ttcctcactg agaaagatat tccggtggcc gagctggtac
    30061 tgcttatcga cccggcttgg ttcagcatcc tgatggaaca taagaagctg atgaacgtcc
    30121 gttacagccg ggatagtggc aacgattacg catggcgtcg tgttggctat gtcgctgggg
    30181 tgcgagttat cgagctgaat agttacccgc aggaagccat taccgggcat gaactgggcg
    30241 ctgattatga cgttaccgca gaggaagcgc tgacgagggc gacgctgttt aaaccttcct
    30301 cggtactgtt gacggtcgaa gcgcagagcg tcatcaatcg ccagtgggat gatgaacgcg
    30361 aaatgacgat ggtgatggac tcatatcgcc tgttcaacgt tgggctgcgt cgtcctgact
    30421 gcgttatcaa tttccgacaa gctgcataac tggaggggct gagtcatggt taatgatgct
    30481 tacgcccctg agctgttgcg atcccttttc ctgtatgacc cggtgacagg tcgcctgcac
    30541 cataaagcta accgacgccg ggttaaggct ggctcctatg ctgactccac tcggcgagct
    30601 gatggctatc gtcaagttgc cctgcggctt gacggcaagc agtaccagct aaaagcccat
    30661 cgcgtggcgt ggattcttgc gcatggcgct attcctgatg gtctgcaagt tgaccacatc
    30721 aacggtatcc gtgacgacaa ccgcctatgc aatctgcgtt tggttacgca gcgggaaaac
    30781 gaccagaacc gacgcaaggc aaggggatat agctggaata agggctgtag caaatgggag
    30841 gcttacatac gcgttgatgg tgtactccgt taccttggct tatttactac cgaagcggcg
    30901 gcgcgtgctg cttatctaaa agcaaaagcc agataccatc ccacaacgcc tgccgacttg
    30961 ttaagagcag cttaaatttt ataggtgata tttaatggct gatgaaaaac gaatcagaca
    31021 gattagagcg ctggcagatg atgacccacg ccgccagcgg tacgtcgagc tattggcgca
    31081 ggctggccgc atgactattc cccggctgca tccgggcgat gctaacagtg tgatgagtca
    31141 gcttgcgacg atgctcgatg cgcctgtccc gatagtcggt gctatgacgg agcacgctgc
    31201 tgcatggcgt gcctgtctgc tagagcaagg gcttggcgat ctgctggctg gcttgagtaa
    31261 gcaggtgctg acaatcttct ttctgctctg ttggagagac agcgccttgc gattggctgg
    31321 taagcaaaag gaactgctgg atatcgctat cgaaggtaag acgcttaaac aagccagtga
    31381 agcgagagca aacgatataa agaggaatgc ttatggcatt gacgattaaa ttaaggtggc
    31441 ggtacatcga accgccgcca atgactggcg cactcgctga cctgaaagtg tgggtgatgg
    31501 ataccggaga gcctgagctt gaagctgaat ttcgtatgct gctggggctg atgcgccgca
    31561 atggtatcag tgacgaacat gttaatgcga tggccgtcga gctttatgct ctggtatgcc
    31621 aacgccagcg cgaagaatac gaagcctgca agcgggctgc ctctgacagt ggagactttg
    31681 aatcatggtt acacggacag accgactact aagaaaagcg atgcggagct atctggaaca
    31741 cctcggcaag atgccgctgg cggaacggat tacaaacgct gctgcattcg aagatgtgta
    31801 tcgcttcctg ttgaatggtt ccggtcggct ggagttgcgc cgcgctgatg tggccgctgt
    31861 ccgtgtcttc ctgtggaatt atcaataccg acgctgtgcg gtgacaggta agccgctgcg
    31921 ccttacgtct gccgtgctag atcactgcca ccgaaccggg cgcgtgcgtg ctgtggtgca
    31981 tcgttctgct aacgctgctg agggtggccg ctacgttggg ttgacgtgcg ggatgagtgt
    32041 cataagatta ggggcaatgg tagatatgta tcgtaaaatt tacaaaggga ttaatattat
    32101 ctaccctaac aaataatttt catatggggc tagaataacc tagcccaatg cgtatttgtt
    32161 ttattctcta attaattcta gcgctccttc tatcaaatac tcccaaataa caccgctttc
    32221 gatgcgctgg ggtttgaaat cacggtctgg gatttgtagc gctaggtttc cttgcccact
    32281 ccagtaatgc tgccatactt cgtagtcaga ctcaaatgtt gatattttat atgcgtgtct
    32341 cattaaagag actatttcca tattaacttt tgctactcgt gtatagggta agtccttcaa
    32401 tcgaaaacgt cgaacagtat ttagatccca attatgcctt tgatgtacct cgacgcaagt
    32461 atcataatca ccaaacgcgt atacagcaga aagtctggag ggatatgttt taaaatatgc
    32521 ctttctattt atttctgcat tatactcaat gcggtagtca ttgattgcac taacatgata
    32581 catggacaat gcagggaaca tgttttcatt tggtaaaaat ggaaggtagt ctagatcacc
    32641 gtctatatat ttttgagggt tgtttccatt catggctaaa gctgagatag ctgctgagga
    32701 tattgaacct acaatattga cgttattatg tgttatagga ttcatatcgt ttacgaaaat
    32761 ataaaactca gaagggcatg tttcttttgt attgatagtt tccataaatt cctcatttaa
    32821 tatttatcac aacttattgt atatcggcag atcaaaaaca tgtttgacca actcgacata
    32881 ttcttttact tcttgtgcgg acacggcatt ttttgagtaa gtcttgaagc tttctgtgac
    32941 tccgcgctca tgaccgacca gtagcgcaat tctatcttct ggcacttgag cacggtcgag
    33001 ttgatgcaca aactcatggc ggagtgaatg gaacactttt ctctgtgtat aagtctcacc
    33061 gagagcttta cgctttgccc gtgtgaactg ctgcgtatgc cacgttgagc gtttcccatc
    33121 ggcccggtct gtaattgacg cgtgatagaa caaaaaaccg ctatgtgtgg tcttgagcaa
    33181 cgacttcacg agagggatta tcttgctgtg tacagggacg attcgcgccg cgctcttggt
    33241 ctttccctcg gtgatctcaa agcacaatac accctcgaca tttttaatgc tgctttcgcg
    33301 tagcgtacat atttcattaa gccgcatacc gctgtatgca ccaatagccg ttaccgcctt
    33361 catctcgtca tcaagtaagg catagatctt tgccagctct cccggtgcga agacttcata
    33421 gctgaccttg ctgctttttg cttcgagcgc atgaccacgc caaggattgt cctgtggtgc
    33481 atcgtgatag cgattgcgtg ccaaatccca aatctgtgcc agtgcgctta tatagttctg
    33541 gatggtttgc ggcgctcgct cagttttgag cttatctaac cagcctgtaa ctatggttcg
    33601 gttaatatcc cgcaactgaa tatctttctt tttcaggtgg gttaacagca cctctacggc
    33661 tttgttggtc ttggatagcg tggtcagctt tctcttctcg ctgtattgga gaagatacag
    33721 atcgcgcatt ttaagtaggg aagggcaagc ctgaaccgat gcaccaaatg atgcgggggc
    33781 ttccttggcg tacacactga cacgccgtag ttcatcaatg atttgatcga cggatgagcc
    33841 tccttgtggc tttagtagat tccgtaagcg agtccattcc agagcaatag catcccgaaa
    33901 caaacgcgct tgacgtatgt ctcgtgtccc tgtgcttttc ataaagtagc gcttcccttg
    33961 gaacaggtgg cgcatgtagc tggggatagc tatccgcacg aggtaattcc cgcagggatc
    34021 taaaactaga taacgatctc tcttgaaact catacaaacc ccggttatgt tgtcggggct
    34081 ttttcatggc taaacagtct gtgaaatgtg ccggggttat gagctttagt ctagctagtt
    34141 caacgagtta acgatgattg ttgaagcaag ctaagccgcc taaacccgtg ccggttcgag
    34201 tccggccttc ggcaccaaaa gtatgtaaat agacctcaac tgaggtcttt ttttatgcct
    34261 gaaatccagt gtttatctat ctttcccgct atattaactc tctcaaggtc aaccgatatc
    34321 aacgtacatc taccaacata tgttggtaca gatgatggta tttccggttc gataatgctt
    34381 gtaccaacag ggagggaata cgatggcatt aacagatatc aaagtcagag cagccaagcc
    34441 aacggataag caatattagc tgactgatgg tggcggtatg catctgcttg tccatccaaa
    34501 tggttctaag tactggcgtt tgcagtaccg ttatgaggga aagcaaaaaa tgctggcact
    34561 tggggtttat cctgaaatca cactagcgga tgccagagta cgtcgtgacg agacgcgtaa
    34621 gctgcttgcg aatggcgtcg atccgggaga caaaaagaaa aatgataagg ttgaacagag
    34681 taaagcacga acctttaaag aagtcgcgat tgagtggcat ggcaccaata aaaagtggtc
    34741 tgaagatcac gcccatcgtg tgctaaaaag tcttgaagat aatctttttg cagcgcttgg
    34801 tgaacgtaat atcgctgagt taaaaactcg agatttatta gcacctatta aggccgtaga
    34861 aatgtctgga cgtcttgaag tggccgctcg tcttcagcag cgcactacag ccatcatgcg
    34921 ctatgcagtg caaagtgggt taattgatta taacccggca caagagatgg ctggggcggt
    34981 tgcttcctgt aatcgacaac atcgtcccgc gcttgaatta aagcgcatcc ctgagttgct
    35041 tacaaaaata gatagctata ctggtaggcc gctaacccga tgggcgatag aactcacttt
    35101 gctgatcttt attcggtcca gtgagctgcg ttttgctcgt tggtcagaga tcgatttcga
    35161 agcgtctata tggactatcc caccggagcg ggagcctatt cctggagtga aacattccca
    35221 tagaggctca aaaatgcgta caacgcatct agtgcctctt tcaacgcaag ctcttgcaat
    35281 tttaaagcag ataaaacagt tttatggggc ccatgacttg atatttattg gtgatcacga
    35341 ttcgcacaaa cccatgagtg agaatacggt aaatagtgcg ttacgggtca tggggtatga
    35401 tacaaaagta gaggtttgtg gtcatggctt tcgaacaatg gcctgtagtt cattggtcga
    35461 atcaggtctg tggtctcgtg atgctgttga acgtcagatg agccacatgg cgcgaaattc
    35521 agtgagggcc gcgtatatcc ataaagcaga gcatctggaa gaacggcgat tgatgctaca
    35581 gtggtgggcc gattttctgg atgtaaacag agaaaggttt atcagtccat ttgaatatgc
    35641 aaagattaat aatccattaa aacagtaatc atcccgggca aatgcccggg aattattcta
    35701 ggattatttt ctttgttaaa aaagacaaac ggtattaact gatgtattta ctatttaccg
    35761 ctccctgcag gggggattat cagaatccgc ggttattcat tatttatata cattcaataa
    35821 aaaagtaacc tcataaaaaa tcctgtactc acagggtgat gcgggataac tgacgtaagg
    35881 agtgctgcag ggatttgatg gtcccttgtg ctgatatgaa tacggatatt ttatacagaa
    35941 tacttttcag tacaagcgac tgtattacag tctgccatat atgattacct gtgatcagga
    36001 agaataagtg gctgaggttt caaactgtct gccggtatat tcctctctcc cttaaaaacc
    36061 ataacaggtt agttatcttc gtctgtcagc ggattgaatg gcggtatatt ttcagtacaa
    36121 ataccggtca tccactgaaa aatacctgcg aaatgacggg cactgatttt tctactgacg
    36181 ggctgatgag acgttatgtc actggcggta ataatcagtg gcacactgta gcctccctgc
    36241 acatgaccat aatgatgaac aggattggca ctgtcgctga ccgacagccc aaggccagaa
    36301 aagtaaagca tggcgaaatg acgtgagtac cggcgaagga taccatcaag ctggccaaga
    36361 aagttatcca gtttactgat gctggcgagg taacaggcaa tttttcgggg atactgctcc
    36421 aggtaattat tcggctagga gttaaggctg tcacacggat ttggatgaga acccatcatg
    36481 tgcaggaaaa ttatcttcgg agaggatgta tccgccagcg cacgttctgt ttcctgtaac
    36541 aacagtttgt catctgtttt acgggaagcg aagctgcctt tcttgaggaa agtggcatgc
    36601 tccgcatcag aagcaataac agagatgcgt gtgtcatgct cccccagttt tccttgattg
    36661 gatatccacc atgtgctgta tcctgctttt gctgccagcg ccaccacatt gttgccggaa
    36721 tcagggttct gctcatagtc ataaatcagt gtccggctca gggaagacac tgtactggag
    36781 gctgtcgagg tataatcgtc aataaataaa ccgggtgccg tattcagcca cggtgtggtt
    36841 ggtacgggat agtcatacac tgacatataa tccctgcgca cactctcacc agtgacaata
    36901 acaatcgtat catataacgg tgctcccggc tggattttcc agttgtcagc cccgtgctac
    36961 tcagttgttc ataacgctgc atttcacgta atgtgtcagt tgtccccaca actgttcctt
    37021 taaccatccg caatggccaa cggtttacag agcataatac gaatagccgt agagccagcc
    37081 agttacggtg gtcgcgatgg tgtgtccgcc agaaaatcac cacgaatacc agaatcgcgg
    37141 cactgaccag aaaatgataa acaggaaaca cggggcggtt cactttgctt tctcctgtag
    37201 tgttttctgt aggttctgaa ccggttctag cggggttgtg aggctgatgg caatcgccgg
    37261 gctcttcctg catgttacac tggtactgca agagggggcc ttcgctcctc tgacattact
    37321 cagtattttc actactacac cccatctgcc atccataaca accgattcac cccctcgcga
    37381 ggcgttgttg cccgtcaccc ggacagacac cgttatggac ttctcttgtt gcacgatcga
    37441 tcaattgtta tgcatgctga caccacaacc ccggtgggta tgaccgatgc ctttctcact
    37501 ctcttcccgg tcatcgtcgg ccttccccgt tattgtggcg tgtcagcacc ctcgacggca
    37561 tttcgatggc tgctcagtgt tcactcacgt tacggcccgc ataacccgtt gacttcctga
    37621 agaagtcgtt tcagggagag attcagccct tttgttacct cctggaccgc ttcttgtcct
    37681 tccagtcgga gcgagagtta gccggggcgg actttcaccg acggaaagat cgtgcatttc
    37741 aaggcacaca caacaatatc tgcgagcgtg ctatccgtcc ggtcgttatg ggacgaaagg
    37801 cctggttgtt cgctggttca ctggtggccg ggaaccgcgc ggcacagata atgagccttc
    37861 tgggaaccgc agtctggagc cacatgcttg gctgacggac gtcctgacgc gtctgccgga
    37921 gtggccggag gagaggttgg ctgagttact gcctcttgag ggctttacct tcttcgggta
    37981 agtgatacct gccgtcaggt gtttgtgcac cgggccataa cctgcagttg ggaattgaac
    38041 gcctgacaac aggaaatgag ctagagcagt aaacacgcgg gctgacaatg ggtcagaata
    38101 tgagttagcc cggcgttatt cctggggtca gtacttcaga tctagcctcc ggtaccagac
    38161 tagctagccc ctacgcaacc aaataaatca accatcgtca gagtgcaatc gttcatatac
    38221 actgtcggtg atgcgctaaa aaatgcgtta atgttgagtt cctcgaaccc ttaccaatta
    38281 agggacatag ccagatacgc aactggctga tgcaagcagt ctaatgtctt tagatccgct
    38341 ggcctgcggg catgacaatg tcaaacagtc aaagattgtg gcgttgacgg tcagtcttgg
    38401 ggacaaaaaa gctcagtatt ctcgttggca accagataat ttaacagttg atgctgaaac
    38461 gaaataggca tcttggttct gtaatgccag tcagttaagc aactgactgg ctctttttcg
    38521 gggctgtggg gtatttccag ggcctctcct ttaccactct cgggaaggcc ctttcccttc
    38581 ttgtcggtaa tttcacaagt tgtcccatac ttgcaagatc gcgcatcagc tccggtatac
    38641 gtcccggtga agcgccctgc aatgtcatca gcattctcat caccattccg catgattctg
    38701 agaaactcag ttgattcggc cagtaacctt tcagatgttc cgccatttta atcatctgat
    38761 atctcaccag attataagcc agtaagacac cccacagctc ttgctccaca agctccggct
    38821 ttttacttct cagcgtcagc ctgctccgtt gcatcgtctg ttttatctcc ctgtatccca
    38881 gtttgatttc ccagcgatga ctgtacagat cccccatttc tcctccgggg aagcgcatgg
    38941 cgtccgtcat cgacgtcagc agatggcaga cttttccttt gcgcgtcacg gtcagcaggc
    39001 gggcagtcac ttcatttccc agtcccggcc acttttttcg tgcctgcggg ctggttttca
    39061 gcttcaccag atgatcgcct ttacccagtt ttctgatctc ttcatattgc gctccctttc
    39121 tgagaggtat catccagtgg cggtgttctc ccgccaggct ccaggcattt aacagtccca
    39181 gtgagtaata acctttatcc attaacgtca gagtgttatc gccggtttgt tctataagtt
    39241 gctcagcaag ctcattttcg ctgttcttca tcgtgccgaa ggctgcagcc gtcagcagat
    39301 ggctggtcag ttccatctgg cagaccattt tgacctgcgg gtagagcgcc gggttcccgg
    39361 catgtgtctg gcgggggaag gctgcatcgt tctctggtgt atccggtgtg cgccagaaca
    39421 caccatcgat ggccagcagg gtcaggccgc accagtgcgg atgcggcgtg gcgttatgcc
    39481 agagctgcgc tgttttcgtg aacacgcggc ggacagcctc acttcccagg cgctggcggg
    39541 cctgaataac ggcactgggg gcaacgaagg ggcgattgcc cggcagcatg atgtccaggc
    39601 gattcacaat ctggtgaaga ggttctttac gctcaagcgc catgccaaca atacaccaga
    39661 ccatcatttc gaggggaaga cggcgcttgc gtagcgttac agtacctgat tcggcaaggc
    39721 aacgagagat gagttcgggg tcgaggtaat cccccagaga agtcagtggg ttacgcagag
    39781 aatcgtaacg ggataccaga tcaagagcct gtccaatgtg cataaaaaaa tccggaaaca
    39841 agtgagcgtt tccggattct tacacagcca ctggatcggt caactgatcc ttaactgatc
    39901 ggcattaatc ttggttctgg tgtttgtaac aaactatcag ctacaaaaat atgctcaatt
    39961 tgtgacatca gtaacaaaac gcgttttgtt atgtggattg cttgtttttt gatctggtat
    40021 aacaggtata aaggtataca gaaaagcaaa gaaatactgc aaaggaaaac agctataacg
    40081 taagctaaag taataacctc tcagtctttc ctcatttgac gaagggagtt ttattcaacc
    40141 tgaacggact acgaaaatga gcacaatgaa taagtcaatt tttgaagcag ggttgaactg
    40201 tgccagtggt ggaatatctg gcgaacattg ttttttgttg gtgacccaaa actgtaggtg
    40261 ggtatcagtt ttatctttca tagagtgaaa tatgttaaga agaaatggag gaaaagattg
    40321 tactgattag gtattgataa caatcaatag tactggcgta tttgaagaca atataattat
    40381 ttctggatat tgttgaggct ccctaatatt tactttaagg gctatattag aataacacag
    40441 gaaacaaata tggcaacagc atggtataaa caagttaatc caccacaacg gaaagctctt
    40501 ttttccgcat ggcttggata tgtatttgat ggctttgatt ttatgatgat attttacatt
    40561 cttcatatta taaaagcaga tcttggcatt acggatattc aggctacttt aatagggaca
    40621 gtggccttca tagccagacc tattggaggt ggtttttttg gtgccatggc tgataaatat
    40681 ggtcgtaagc caatgatgat gtgggcaatt ttcatttact cagtcggaac aggccttagc
    40741 ggtattgcta caaacttata tatgctcgca gtttgccgtt ttattgttgg cttagggatg
    40801 tctggtgaat atgcatgtgc ttcaacttat gcggtagaaa gttggcctaa aaatcttcaa
    40861 tctaaagcta gtgctttttt ggtaagtggt ttttctgttg gaaatattat tgcggcacaa
    40921 ataatccctc agtttgctga agtatatgga tggagaaact ctttttttat aggcctgtta
    40981 ccagttttac tagttctttg gatcagaaaa agtgctccag aaagtcagga gtggattgaa
    41041 gataaatata aggataaatc aacatttttg tctgtcttca gaaaaccaca tctttcaatc
    41101 tctatgatcg ttttcctcgt ctgtttttgt ctatttggtg caaactggcc gataaacgga
    41161 ctacttcctt cctacctggc agataatgga gttaatacag tggtcatttc aactctgatg
    41221 acaatagcag gtttaggaac actgacaggt acaatatttt ttggttttgt tggtgataag
    41281 attggtgtaa aaaaagcctt tgtagtcggt ctaataactt catttatttt cctttgtcct
    41341 ctttttttta tttctgtgaa aaactcttct cttataggat tatgtctttt tggattaatg
    41401 tttacaaatt taggtattgc agggttggtt ccaaaattta tatatgatta ctttccaaca
    41461 aaattaagag gattagggac cggtcttatt tataacttag gggcaactgg aggaatggcc
    41521 gcacctgtat tagctacata catttcagga tattatggct taggtgtttc attattcatt
    41581 gttacggttg cattctctgc cttattaatt ttgttagttg gttttgatat tccaggtaaa
    41641 atttataaac tatccgtggc taaatgattg gaggctttat gattaattat ggcgttgttg
    41701 gtgttggata ctttggcgct gaattagctc gttttatgaa tatgcatgat aatgcaaaaa
    41761 ttacatgtgt atacgatcct gaaaatggag aaaatattgc ccgtgaattg cagtgtatca
    41821 atatgtcaag cttggatgct ttagtctcaa gtaaattagt cgattgcgtg atcgtagcca
    41881 ccccaaatta tctgcataaa gaaccagtaa ttaaagcagc aaagaataag aagcatgttt
    41941 tttgtgaaaa accaattgca ttaagttatg aagattgtgt ggatatggtc aaagcgtgta
    42001 aagaagctgg tgtgaccttt atggccgggc atattatgaa ttttttcaat ggggttcaat
    42061 atgcacggaa gttaattaaa gaaggtgtta tcggcgaaat attatcatgt catactaaga
    42121 gaaatggctg ggaaaacaaa caagagagac tttcctggaa aaagatgaaa gaacaatctg
    42181 gtggacatct atatcatcat atacatgagt tagattgtgt tcagcattta cttggagaaa
    42241 taccagagac ggttactatg attggtggaa atttggccca ttctggtcca ggatttggca
    42301 atgaagatga tatgttattt atgaccttgg aattcccgtc aggaaaacta gcaaccttag
    42361 agtgggggag tgcatttaac tggccggaac attatgtcat catcaatgga actaaaggct
    42421 ctattaaaat tgatatgcaa gaaacagcag ggtcacttag gattggcggt cagacaaagc
    42481 attttttggt ccatgaaaca caagaagaag atgatgatcg tcggaaaggc aatatgacct
    42541 cagaaatgga tggcgctata gcatatggtc atccaggaaa aaaaacacca ttatggcttg
    42601 ccagtttaat aagaaaggag acgttattcc tccataatat cctctgtggt gcaaaacctg
    42661 aagaagatta tattgacctt ctcaatggtg aggcggccat gtcggcgatt gctactgctg
    42721 atgctgccac tctttcaaga tcgcaggaca ggaaagtgaa aatcagtgag atcattaaac
    42781 atacatcagt aatgtaaaaa ctatacgcct cttgatttaa ttcaggaggc cttttatgta
    42841 tgatcaagaa ttttatcttc gataatctca taattttagc agtgccattt atgataaaaa
    42901 catcccttaa aaccaatcta atctttttct tcctctgtgt atttgttccc catatggcgt
    42961 cataatttac gctacgtaat acgggatagt tacgatacgc agcgatagcg ctaagtttta
    43021 gttaaaatcc cccatcagcg ggatatgggg atctctggga ggttcaggtt ttagcatcgc
    43081 gactttaata cgacagacgc tggtgacgat gatgccaagt agtgactctt ccgaataagg
    43141 ttcccactga ccaattggcg cgcgcttcgc aataaaatct cccttcgccc agtattggga
    43201 aaagtagata cattcaaact gtgtacgctg tttcgtctca cagttcacga cattgctggc
    43261 cgatgagctg accattgggc ctatccgggc tgtcccgatg gatattacgc cgaagtatgt
    43321 gggaattgcc agcggattga tgaacgccgg ttccgctgtc gccgacatta tttcacctat
    43381 cgcctttggc attattatcg acaaaacagg caactggagt ttaccattct acggttccgt
    43441 cgcattgctt gttattggta tattcctgac gttcttcatg cgtccagata agtcattgta
    43501 aaagtcgaat cagggctgaa gtggcacact gaatttggcc acctgaacag aggtgatatg
    43561 ctcacctcag aacaacacag gtgctccaat gaaaaaaaga aatttcagcg cagagtttaa
    43621 acgcgaatcc gctcaactgg ttgttgacca gaaatacacg gtggcagatg ccgcgaaagc
    43681 tatggatgtt ggcctttcca caatgacaag atgggtcaaa caactgcgtg atgagcgtca
    43741 gggcaaaaca ccaaaagcct ctccgataac accagaacaa atcgaaatac gtaagctgag
    43801 gaaaaagcta caacgcattg aaatggagaa tgaaatatta aaaaggctac cgcgctcttg
    43861 atgtcagact ccctgaacag ttctcgataa tcgggaaact cagagcgcat tatcctgtgg
    43921 tcacactctg ccatgtgttc ggggttcatc gcagcagcta cagatactgg aaaaaccgtc
    43981 ctgaaaaacc agacggcaga cgggctgtat tacgcagtca ggtacttgag ctacatggca
    44041 tcagccacgg ttcggccgga gcaagaagca tcgccacaat ggcaacccgg agaggctacc
    44101 agatgggacg ctggcttgct ggcaggctca tgaaagagct ggggctggtc agctgtcagc
    44161 agccgactca ccggtataaa cgtggtggtc atgaacatgt tgctatccct aactaccttg
    44221 aaaggcagtt cgccgtgacc gagccaaatc aggtgtggtg cggtgatgtg acctatatct
    44281 ggacgggtaa gcgctgggcg tacctcgccg ttgttctcga cctgttcgca agaaaaccag
    44341 t
//
LOCUS       DAYPHN010000019        44320 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000019 DAYPHN010000000
VERSION     DAYPHN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.019"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            115..687
                     /locus_tag="ACG0BW_001065"
     CDS             115..687
                     /locus_tag="ACG0BW_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7258055.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQINISGLVSDNTCETK
                     VDGGKQDGLVLLQTATVAEITAGVSETAVGAKAKPFSITVDCSKATPGGQPTLTFGSV
                     FFGNSRGTLNNDMSITTPSSGVNIALHNIDGTTVKQVKINNASDAYTKALTNNVAVFD
                     FKASYVREDDSTPATAGYVRTNTAYTITYN"
     gene            777..1511
                     /locus_tag="ACG0BW_001066"
     CDS             777..1511
                     /locus_tag="ACG0BW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFK7258056.1"
                     /translation="MLLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVS
                     VRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGST
                     VLPSDKESVFWFNVLEVPPKPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPL
                     ALKWKWAEGKSGLSVYNPTSYYVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSG
                     GKATSGKVHFSAINDFGGAIEGDVNL"
     gene            1546..4146
                     /locus_tag="ACG0BW_001067"
     CDS             1546..4146
                     /locus_tag="ACG0BW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFK7258057.1"
                     /translation="MKTKTSTKRHNLTRIAMYCSIMYSGATIGAESVEYDPTFLMGGN
                     ASSIDVSRYSDGNPTLPGVYDVSIYVNEQPVANLEIPFIAIPDKKNAQACITLKNLLQ
                     LHIKTPPADEENTILLPRDETLGNCLDLSLAIPKSSVNYDPSEQRLDINVPQAWVMKN
                     YQNYVDPSLWENGINAATLSYNINAYRSENSNYTNDSVYTSFNGGVNLGAWRLRSSGN
                     YSWRNDAGSNVEFMNRYVQRDITAIRSQLIMGESYTTGETFDSVSIRGIRLYSDSRML
                     PPVLANFAPTIRGVANTNAKVSITQSGYKIYETTVPPGPFVIDDLSPSGYGSDLIVTI
                     EEADGSKRTFSQPFSSVIQMLRPGVSRWDISGGQINKDDLHHEPNLLQATYYRGLSNL
                     FTGYTGFQVTDNHYAAGLLGIGMNTSVGAISFDVTHSSVDIPDDKRYQGQSYRISWNK
                     FFDLTDTSLNIAAYRYSTQDYLGLNDALTLIDEVEHPTQDLDPKTMRNYGRMKNQFTV
                     SINQPLRFGKDDYGSFYTSGSWSDYWGGSKSRSNYSVGYSNSASWGSYSISAQRSWDE
                     YSQTENSIYLSFSIPIEKLMGTEHRDSGFQSIDTQLSTNMDGNNQFNMSSSGYSNDNR
                     VSYSVNAGYGMNKTGKDLSNIGGYASYESPWGTLAGSVSATSDNNRQYSVNTDGGFVL
                     HSGGLTFSNDSFSDNDTIALVKAPGAKGARINYGNNTVDRWGYGVTSALSPYQENKIA
                     LDTENLENDIEMKSTSTVAVPRQGSVIFAGFETNQGQSAIMNIKRSDGKGIPFAADIH
                     DETNAIIGNVGQGGQAFVRGIQQQGTIKITWLEASMPKTCIAQYQQPNVTSEKIQQTI
                     ILNNILCQLQ"
     gene            4165..4725
                     /locus_tag="ACG0BW_001068"
     CDS             4165..4725
                     /locus_tag="ACG0BW_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7258058.1"
                     /translation="MIKNTRYKIVLLAGIFLLPGALAQDINVDFVATIKGTTCNITLE
                     GTRVTADGNNQYTLRILNVALDKIINKMPEAQADFKLVANCDGNIGKITTSLAGNPSV
                     ELPYLISPLTSDNSSTTEYIAMGIKLRNASDTEFIPPDGSKKISWGEGLTKPELEMTV
                     ALRETRPGSGRAGNFKALATFNFTYE"
     gene            4739..5338
                     /locus_tag="ACG0BW_001069"
     CDS             4739..5338
                     /locus_tag="ACG0BW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFK7258059.1"
                     /translation="MQMKSLHYGIFASITLSLLLFSTFSIAITDNIGLNVMTTVENRT
                     CQSEISNQGLVNLGTVGVGYFASNVSAEDNYPGGKTFTVTVKDCPSAGTTSSQLHIEF
                     KPVSGVMATGSQQIFANNHVGGAQNVGIVIFSQNNQSSDTVNVLSPTGQFRTVWPVAS
                     ANMNNSSWQFYTRMQKIDPLLPVGAGFVTSNVLVDIYYQ"
     gene            5390..6640
                     /locus_tag="ACG0BW_001070"
     CDS             5390..6640
                     /locus_tag="ACG0BW_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFK7258060.1"
                     /translation="MKSAFRYFLFLWIITASINNVNAENLTFMVGDDAGVGNLNGPSG
                     DKLISFSYPVGGVNNIVFYNPTSTTPTNAKIKLYWSELDTSSSRGFVYCTSKNNSSGM
                     PITVRHNMVDSGKMADGHRLYETNVTGLYYTLMLNRFWSYELRSSSPDIYVGDDGWHT
                     VQFSITGDYIRGQCNTSRYQAVGGLLHDATVEFYTDSSFNPAPNTNVQLKHTGDYLYS
                     FANEGPGVPNVSASKMLKINFNLTDINIQLPTCFTSILLGESVTRSTVNLGEYTVGQI
                     TSDSATPVNFQISLQNCVRISSIKTKLKSNVVGVNNPLLLGNTLTGTDDAKGAGVLIE
                     GLPNSKNSQNFTLKPNDETSSYNDAEDDPGENNGIYNPDYPNANGRTTTQDLKFQATL
                     KRDGNATVKPGGFKATSTFQVTYE"
     gene            6754..7548
                     /gene="panB"
                     /locus_tag="ACG0BW_001071"
     CDS             6754..7548
                     /gene="panB"
                     /locus_tag="ACG0BW_001071"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFK7258061.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7560..8411
                     /gene="panC"
                     /locus_tag="ACG0BW_001072"
     CDS             7560..8411
                     /gene="panC"
                     /locus_tag="ACG0BW_001072"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFK7258062.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELV"
     gene            complement(8493..8708)
                     /locus_tag="ACG0BW_001073"
     CDS             complement(8493..8708)
                     /locus_tag="ACG0BW_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7258063.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLRKVRLAQRLL
                     SKGMSREDVAEMATLSLTEVDKLINSN"
     gene            complement(8777..9721)
                     /locus_tag="ACG0BW_001074"
     CDS             complement(8777..9721)
                     /locus_tag="ACG0BW_001074"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFK7258064.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEGRQEVRQEVRQEVRQEVIQEV
                     TQEVSQEFAQRLLSKGMSREDVAEMANLPLAEIDKLIN"
     gene            9995..10375
                     /gene="panD"
                     /locus_tag="ACG0BW_001075"
     CDS             9995..10375
                     /gene="panD"
                     /locus_tag="ACG0BW_001075"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFK7258065.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10379..11608)
                     /gene="yadE"
                     /locus_tag="ACG0BW_001076"
     CDS             complement(10379..11608)
                     /gene="yadE"
                     /locus_tag="ACG0BW_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFK7258066.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVFNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYVTLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11672..12112)
                     /gene="yadI"
                     /locus_tag="ACG0BW_001077"
     CDS             complement(11672..12112)
                     /gene="yadI"
                     /locus_tag="ACG0BW_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFK7258067.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGVLLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12217..12987)
                     /gene="yadH"
                     /locus_tag="ACG0BW_001078"
     CDS             complement(12217..12987)
                     /gene="yadH"
                     /locus_tag="ACG0BW_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7258068.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12984..13910)
                     /gene="yadG"
                     /locus_tag="ACG0BW_001079"
     CDS             complement(12984..13910)
                     /gene="yadG"
                     /locus_tag="ACG0BW_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7258069.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14019..14681
                     /gene="can"
                     /locus_tag="ACG0BW_001080"
     CDS             14019..14681
                     /gene="can"
                     /locus_tag="ACG0BW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFK7258070.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14722..15258)
                     /gene="hpt"
                     /locus_tag="ACG0BW_001081"
     CDS             complement(14722..15258)
                     /gene="hpt"
                     /locus_tag="ACG0BW_001081"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFK7258071.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15464..17854
                     /gene="gcd"
                     /locus_tag="ACG0BW_001082"
     CDS             15464..17854
                     /gene="gcd"
                     /locus_tag="ACG0BW_001082"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFK7258072.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17901..19451)
                     /gene="cueO"
                     /locus_tag="ACG0BW_001083"
     CDS             complement(17901..19451)
                     /gene="cueO"
                     /locus_tag="ACG0BW_001083"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFK7258073.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFSGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHEAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19617..19964
                     /gene="yacC"
                     /locus_tag="ACG0BW_001084"
     CDS             19617..19964
                     /gene="yacC"
                     /locus_tag="ACG0BW_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFK7258074.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20070..20936
                     /gene="speE"
                     /locus_tag="ACG0BW_001085"
     CDS             20070..20936
                     /gene="speE"
                     /locus_tag="ACG0BW_001085"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFK7258075.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20952..21746
                     /gene="speD"
                     /locus_tag="ACG0BW_001086"
     CDS             20952..21746
                     /gene="speD"
                     /locus_tag="ACG0BW_001086"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFK7258076.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21784..22146)
                     /gene="yacL"
                     /locus_tag="ACG0BW_001087"
     CDS             complement(21784..22146)
                     /gene="yacL"
                     /locus_tag="ACG0BW_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFK7258077.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22343..24940)
                     /gene="acnB"
                     /locus_tag="ACG0BW_001088"
     CDS             complement(22343..24940)
                     /gene="acnB"
                     /locus_tag="ACG0BW_001088"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFK7258078.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25295..27148
                     /gene="yacH"
                     /locus_tag="ACG0BW_001089"
     CDS             25295..27148
                     /gene="yacH"
                     /locus_tag="ACG0BW_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFK7258079.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QNAPDISAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPVTQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDNNMAWRHNPNYRDGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPALSRDSQRQAAASQFQQRTHTAPVITRDTQRQAAAQRFNEAEHYGSYDDFREFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRKEAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRKKVQESRPQRLNDSNRTARLNNEQRSAIRERLSERGARRMER"
     gene            complement(27219..28643)
                     /gene="lpdA"
                     /locus_tag="ACG0BW_001090"
     CDS             complement(27219..28643)
                     /gene="lpdA"
                     /locus_tag="ACG0BW_001090"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFK7258080.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28851..30743)
                     /gene="aceF"
                     /locus_tag="ACG0BW_001091"
     CDS             complement(28851..30743)
                     /gene="aceF"
                     /locus_tag="ACG0BW_001091"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFK7258081.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     GKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30758..33421)
                     /gene="aceE"
                     /locus_tag="ACG0BW_001092"
     CDS             complement(30758..33421)
                     /gene="aceE"
                     /locus_tag="ACG0BW_001092"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFK7258082.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33582..34346)
                     /gene="pdhR"
                     /locus_tag="ACG0BW_001093"
     CDS             complement(33582..34346)
                     /gene="pdhR"
                     /locus_tag="ACG0BW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFK7258083.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            34887..36260
                     /gene="aroP"
                     /locus_tag="ACG0BW_001094"
     CDS             34887..36260
                     /gene="aroP"
                     /locus_tag="ACG0BW_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFK7258084.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36303..37157)
                     /gene="ampE"
                     /locus_tag="ACG0BW_001095"
     CDS             complement(36303..37157)
                     /gene="ampE"
                     /locus_tag="ACG0BW_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFK7258085.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37154..37705)
                     /gene="ampD"
                     /locus_tag="ACG0BW_001096"
     CDS             complement(37154..37705)
                     /gene="ampD"
                     /locus_tag="ACG0BW_001096"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFK7258086.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            37793..38686
                     /gene="nadC"
                     /locus_tag="ACG0BW_001097"
     CDS             37793..38686
                     /gene="nadC"
                     /locus_tag="ACG0BW_001097"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFK7258087.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFC"
     gene            38889..39329
                     /gene="ppdD"
                     /locus_tag="ACG0BW_001098"
     CDS             38889..39329
                     /gene="ppdD"
                     /locus_tag="ACG0BW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFK7258088.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            39339..40724
                     /gene="gspE"
                     /locus_tag="ACG0BW_001099"
     CDS             39339..40724
                     /gene="gspE"
                     /locus_tag="ACG0BW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFK7258089.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDVGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRLQQGESIHIPDNVWSSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            40714..41916
                     /gene="hofC"
                     /locus_tag="ACG0BW_001100"
     CDS             40714..41916
                     /gene="hofC"
                     /locus_tag="ACG0BW_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFK7258090.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(41951..42994)
                     /gene="guaC"
                     /locus_tag="ACG0BW_001101"
     CDS             complement(41951..42994)
                     /gene="guaC"
                     /locus_tag="ACG0BW_001101"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFK7258091.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(43150..43194)
                     /locus_tag="ACG0BW_001102"
     CDS             complement(43150..43194)
                     /locus_tag="ACG0BW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258092.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43219..43839
                     /gene="coaE"
                     /locus_tag="ACG0BW_001103"
     CDS             43219..43839
                     /gene="coaE"
                     /locus_tag="ACG0BW_001103"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFK7258093.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDASPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            43839..>44320
                     /locus_tag="ACG0BW_001104"
     CDS             43839..>44320
                     /locus_tag="ACG0BW_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFK7258094.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIADHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPSLHIWLHLPQAQRDSQVETWIAS
                     L"
BASE COUNT        11058 a        11640 c        10810 g        10812 t
ORIGIN      
        1 cagtaaatct tcacattcat cattaacaaa acaatctatt tttctctatc ggggttcttt
       61 gtctgaaagc acccgattgt agttaaactc atggaatata attcaaggat tattatgtct
      121 aaaaaattag gttttgccct gtctggtctt atgctggcaa tggttgcagg tactgcttct
      181 gctgatatgg acggtggtca gattaacatc agtggcttgg ttagcgataa cacctgtgaa
      241 acaaaagttg atggcggcaa gcaagatggc ctggttcttc tgcaaactgc aacggttgct
      301 gaaattaccg ctggtgtatc tgaaactgct gtcggtgcca aagcgaaacc gtttagcatt
      361 actgtagact gcagcaaagc gactccggga ggccaaccga ctctgacatt cggttcagtt
      421 ttctttggta acagtcgtgg cactttgaat aacgatatgt ccatcaccac cccgtcatct
      481 ggtgtgaata tcgccctgca caacattgac ggcaccactg ttaaacaagt caaaattaat
      541 aatgcatctg atgcttacac taaagcatta accaataatg ttgcagtctt tgacttcaaa
      601 gcctcctatg ttcgtgaaga cgactcaaca ccagcaacgg ctggttatgt aagaaccaac
      661 acagcttaca ccatcaccta caattaagtt attcgttggt gatttaaatt actacaaggg
      721 tgcgacacct attgtcgccc ccttgtcttg aaaacaattc atgtcatgga tggtatattt
      781 tgttaaataa aaagaatatt gtcgcattat gtgttgccag tagtatggca tttagtatgc
      841 ctgttatggc tgatattgtt atttccggta cacgcgtcat ttataaaagt gatcaaaaaa
      901 gcgttagcgt acgcctggag aataaaggca atagccatct gttagtacaa agctggctgg
      961 ataccggtga cgataacgct gagccgggaa gcattaatgt accatttacg gcaacacctc
     1021 ccgtttcacg tattgatgct aaacgcggtc aaaccatcaa attgatgtat accggcagca
     1081 ccgtactccc cagcgacaaa gaaagcgtat tctggtttaa cgtgctggaa gtgcccccaa
     1141 aaccagatgc ttcaaaagta gagaatcaga gcctgttaca actggctttc cgtacccgta
     1201 tcaagctgtt ctatcgtccg gcaggattaa acggtaaccc gtctgaagca ccattagccc
     1261 tgaaatggaa atgggctgaa gggaagtctg gcctgagtgt atataaccca acatcttatt
     1321 acgtctcttt caacagtgca gagttaatcg ctggcggtaa gtcgaacccg ctcaatgtaa
     1381 aaatggttgc accgaaaagc gaagagattt tcaccataag cggttcaggc gggaaagcaa
     1441 cgtcaggaaa ggtacatttc tccgcgatta atgattttgg cggcgcaatt gaaggcgatg
     1501 tcaatttgta agaccaggga ccttatgcag caggaagcca atgtaatgaa aacaaagaca
     1561 tcgactaaac gccataatct tacgcgtatc gcaatgtatt gctcaatcat gtatagcggc
     1621 gctaccatcg gagccgaaag tgttgagtat gaccctacct ttttaatggg tggcaacgct
     1681 tccagtattg atgttagccg ctatagcgat ggcaacccga ctttgcctgg tgtttacgat
     1741 gttagcatct atgttaacga gcaacctgtt gctaacctgg agatcccctt tatcgcgatc
     1801 cccgataaaa agaatgctca agcctgtatt accctgaaaa atctactaca actgcatatc
     1861 aagacacccc cggcagacga ggaaaatact atccttcttc cacgggatga aacgcttggc
     1921 aactgtcttg acctgtcgtt agccatacct aaatcctcag tgaactatga cccctctgaa
     1981 caacgtctgg atatcaatgt tccccaggca tgggtgatga agaattatca aaactatgtg
     2041 gacccatcac tttgggaaaa tggcattaac gcggctacgc tgtcttataa catcaacgct
     2101 tatcgcagtg aaaattcaaa ttatacaaat gatagcgttt atacatcgtt caatggtggc
     2161 gtcaatttag gggcctggcg tctgcgtagc agcggtaact atagctggag aaacgacgcg
     2221 ggaagcaatg ttgagtttat gaatcgctac gtccagcgcg acattaccgc tatccgctcg
     2281 cagctcatta tgggggaatc ctacaccaca ggtgaaacgt tcgactccgt tagtatccgc
     2341 ggcattcgct tatacagcga cagccgaatg ctgccgcctg tattggcaaa cttcgccccg
     2401 acaattcgtg gtgtagcaaa tactaacgcc aaagtcagca tcacccagag tggatacaaa
     2461 atttatgaaa ccactgttcc accgggacct tttgtcattg atgatctcag cccgtcaggg
     2521 tatggcagcg atcttatcgt aaccattgaa gaagctgatg gcagcaagcg tacattttct
     2581 cagccatttt cttcagtaat tcaaatgctg cgcccgggtg tttctcgctg ggatatcagc
     2641 ggcgggcaaa ttaataaaga cgacttgcat catgaaccaa acttactaca agcgacctat
     2701 taccgtggtt taagtaacct gttcactggc tatactggtt ttcaggtcac agataatcat
     2761 tatgctgcag gtttacttgg cattgggatg aatacttcag tcggcgcaat ttccttcgat
     2821 gtcacccact ccagtgtcga tataccggat gataaaaggt accaggggca aagctatcgc
     2881 atctcctgga acaaattttt tgacctgaca gatacatcat taaacatcgc ggcttaccgc
     2941 tactccacgc aggactatct gggcctgaat gatgcattaa cacttattga tgaagttgaa
     3001 catcccactc aggatcttga tcccaaaact atgcgcaact acgggcgcat gaaaaaccag
     3061 ttcaccgtaa gcattaacca accgctacgc tttgggaagg atgattatgg ctcgttctat
     3121 acctctggaa gctggtctga ttactggggg ggcagtaaga gccgtagcaa ctattcggtc
     3181 ggctacagta acagcgcttc atggggaagc tacagtatca gcgctcaacg atcctgggat
     3241 gagtacagcc aaacagaaaa cagtatttac ctcagctttt cgatacctat cgaaaagctg
     3301 atgggtacgg agcaccgtga ttccggtttc caaagtattg atacacaact tagtaccaat
     3361 atggatggta ataatcagtt caacatgagc agtagcggtt atagcaacga taaccgcgtg
     3421 agctatagcg tgaacgcggg ttatgggatg aataaaaccg gcaaagatct cagcaacatc
     3481 gggggttatg ccagctatga atcgccatgg ggaacgctag ctgggtcagt ctctgctacc
     3541 agcgataata accgccagta ctccgttaat acagatggtg gatttgtgtt gcacagcgga
     3601 ggactgacgt tcagcaacga cagtttcagt gataatgaca ccattgcttt ggttaaagca
     3661 ccgggtgcaa aaggggcgcg tattaactac ggcaacaata ccgttgaccg ttggggatat
     3721 ggcgtaacca gtgctctttc accataccag gaaaacaaaa ttgctctgga cactgaaaac
     3781 ctggaaaacg acatcgaaat gaagagcacc agtacggttg ccgtaccacg ccagggctcg
     3841 gttatctttg ctggttttga aaccaatcag ggacaatctg cgattatgaa tatcaaacgc
     3901 agtgacggaa aaggtatccc cttcgccgcc gatattcatg atgaaacaaa tgcaatcatt
     3961 ggcaacgttg ggcagggcgg gcaggcattt gttcgcggta ttcagcagca gggcacgata
     4021 aaaatcacct ggctggaagc cagcatgccc aaaacctgta ttgcgcagta ccagcaaccc
     4081 aatgtgacat cggagaaaat ccagcaaacg attattctca ataatattct ttgccagctc
     4141 caataatacg aaagagagat gacaatgata aaaaacacac ggtataaaat agtactcctg
     4201 gcaggcattt ttttactgcc cggcgcactg gcacaagata ttaatgttga ttttgttgcc
     4261 acaatcaaag gcacgacatg taatatcact ctggaaggga cacgcgtcac cgctgatggc
     4321 aataatcagt acacgctacg gatattaaat gttgctctgg ataaaatcat aaacaagatg
     4381 ccagaagcac aagccgactt taaactggtc gctaattgtg atggtaacat aggcaagatt
     4441 acgacatcgc tggcagggaa tccttcagta gagttaccct atttaatttc tccattaact
     4501 tcagataatt catccaccac agaatatatt gctatgggga ttaaacttcg aaatgcctct
     4561 gacactgaat ttattccccc agatggttct aaaaagattt cctggggaga gggattaaca
     4621 aaaccggaac ttgagatgac ggtagcactt cgcgaaaccc gaccaggttc aggaagagca
     4681 ggtaatttta aagcacttgc gacattcaat ttcacctacg aataaaagga taataataat
     4741 gcagatgaaa tcattacatt atgggatatt tgcaagcatt actctgagct tattattatt
     4801 cagcacgttt tctatagcca tcacggataa cattggccta aacgttatga ccactgttga
     4861 aaacagaact tgtcaaagcg aaatcagcaa ccaggggttg gttaaccttg gcactgttgg
     4921 agtgggatat tttgccagta atgttagtgc ggaagataat tatccaggcg gaaagacctt
     4981 tactgtgaca gtaaaagatt gcccctccgc tggcacaaca tcaagccagt tgcatataga
     5041 atttaaacct gtcagtggtg taatggccac tggtagccaa cagatattcg ccaataatca
     5101 tgtcggcggt gcacaaaatg taggaatcgt gatttttagc caaaataatc agtcatcgga
     5161 cacagtgaat gtgctgtcac caacagggca gtttcgcaca gtctggccag ttgcctctgc
     5221 aaacatgaat aattcatcgt ggcaatttta tacacgaatg caaaaaattg atccgttact
     5281 tcccgttggt gcaggatttg tcaccagcaa tgtattagtt gatatttatt atcaataaat
     5341 gccagacata acgcatctac aaatattatt ttttcaaagg agtgataaga tgaagtcagc
     5401 gttccgatac tttctttttt tatggattat cacagccagc attaataatg taaatgctga
     5461 aaatcttact tttatggtcg gtgacgatgc tggcgtagga aaccttaatg gcccatcagg
     5521 cgataaacta atatcatttt cctatccggt tggtggagta aacaatattg tattttataa
     5581 tccaacatcc accacaccga ccaatgcaaa aataaaatta tattggagtg aactggacac
     5641 atcaagtagc cgtgggtttg tatattgtac ttcaaaaaat aattccagcg gtatgccaat
     5701 tactgtccgg cataacatgg ttgactcagg aaaaatggca gatgggcaca gattatatga
     5761 aactaatgta accggtctat attatacgtt aatgctcaat agattctggt cgtatgaact
     5821 tcgctcaagc tctccagata tatacgttgg agacgatggc tggcacactg tacaattttc
     5881 tattacgggt gattatatac gaggtcaatg taatacttcc aggtatcaag cagtcggagg
     5941 tcttttgcac gatgccacgg tggaattcta taccgacagc agttttaacc ctgctccaaa
     6001 tactaacgtg caattaaaac atactggtga ttatttgtat tcattcgcaa atgaagggcc
     6061 aggggttcca aatgtaagtg catctaagat gctaaaaatc aattttaatt taacagacat
     6121 caatatccaa ctgccaacat gctttacgtc tatccttttg ggtgagagcg taacgcgatc
     6181 aaccgtaaat ctcggggaat atactgttgg acagatcacc tcagattctg caacacctgt
     6241 taattttcag atctcgctac aaaattgtgt tcgtataagc agtattaaaa caaaactaaa
     6301 atctaatgta gtaggagtga ataatcctct gttgcttggt aatactttga caggaaccga
     6361 tgatgcaaaa ggtgccggag tacttattga agggttaccc aacagcaaaa attcacaaaa
     6421 tttcacgctt aagccaaatg atgagacatc aagctataac gatgctgaag atgatcctgg
     6481 agaaaacaat ggaatttata acccagatta tcctaatgcc aacggtcgta ctacaaccca
     6541 ggacctgaaa tttcaggcaa cattaaaacg cgatggtaat gcaactgtga aacccggcgg
     6601 atttaaggct accagtacat tccaggtcac ctacgagtaa caggtttaac cgcaccgcca
     6661 tcatctggcg gtgctgtaat tgataaatca ttgcccccac caatgtgact gataaaatgc
     6721 cagacagcac gacattactt atcaggatac gttatgaaac caaccactat tgcttcattg
     6781 caaaaatgca aacaagaaaa aaaacgtttc gcgaccatca ccgcttacga ctatagcttc
     6841 gcaaaactgt ttgccgatga agggcttaac gtcatgctgg ttggcgattc actgggcatg
     6901 acggttcagg ggcatgactc aactctgcca gttaccgttg ccgatatcgc ctaccacact
     6961 gctgctgtac gtcgcggtgc gccaaactgt ctgctgctgg ctgatctgcc gtttatggcg
     7021 tatgccacac cagaacaagc ctttgaaaac gccgcgactg tcatgcgtgc cggtgccaac
     7081 atggtcaaaa ttgagggcgg tgagtggctg gtagaaaccg tacaaatgct gaccgaacgt
     7141 gccgttcctg tttgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc
     7201 tacaaagtcc aggggcgcgg cgatgaagcg gccgatcgac tgctcagcga tgcattagct
     7261 ttagaagctg ctggggcaca actgctggta ctggagtgcg tgccggttga actggcaaaa
     7321 cgtattaccg aagcactggc gatcccggtt attggcattg gcgcaggcaa cgtcactgac
     7381 gggcaaattc tcgtgatgca cgacgcattc ggcattaccg gcggtcatat tcctaaattc
     7441 gccaaaaatt tcctcgccga aacgggcgac atccgcgcgg ctgtgcggca gtatatggct
     7501 gaagtggagt ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtgacgttg
     7561 tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat ccgccgcctg cgtatggaag
     7621 gtaagcgcgt ggcgctggtg cccaccatgg gtaacctgca cgacggccat atgaagctgg
     7681 tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc
     7741 agttcgaccg cccggaagat ctggcacgtt atccacggac cttgcaagag gactgcgaaa
     7801 agctgaacaa gcgcaaagtg gatttagttt tcgccccttc ggtaaaagag atctacccga
     7861 acggtacaga aacccacacc tatgttgatg ttcccggtct ttctaccatg ctggaaggtg
     7921 ccagccgtcc gggacatttt cgcggcgttt cgactatcgt cagcaagctg ttcaatctgg
     7981 tccagccgga catcgcctgc ttcggtgaaa aagattttca gcaactggcg ctgatccgca
     8041 aaatggttgc tgatatgggc tttgatattg agattgtcgg tgtgccaatt atgcgcgcca
     8101 aagacggtct ggcgctgagt tcccgtaacg gttatctgac ggcggaacaa cgcaaaattg
     8161 cgcctggtct gtacaaggtt ttaagttcga ttgccgacaa attgcaggct ggcgaacggg
     8221 atctcgatga aattattact attgccgggc aagaactgaa tgaaaaaggc ttccgcgccg
     8281 atgatattca gattcgtgat gccgacacct tgctggaggt ctctgaaaac agcaaacgtg
     8341 cagtgattct ggtagccgcc tggcttggcg atgcccgcct gatcgacaac aaaatagtcg
     8401 agctggtgta atacttaact ggcgctacgg ctgatggcgc cagttattca ttgccatgtt
     8461 atctgtaagc gataattcat aacgcagaca acttagttag agttaattag cttatcaacc
     8521 tctgtaagag ataaagttgc catctctgca acgtcttccc gagacattcc tttactcaga
     8581 agacgctgtg ccagccttac cttccggagt ccctctttat atcccttctg aaatcccttc
     8641 tgaaatccct tctcttcaaa ccactgcgcc aacgtctgcc tggtttctct ccgtgtcttt
     8701 atttccatag gttcccttaa ttatctggtc agttaacaat aacaattatc tggctataac
     8761 tgatgatgca gacaacttag ttaatcagct tatcaatctc ggcaaggggt aaatttgcca
     8821 tctctgcaac gtcttcccga gacattcctt tactcagaag acgctgggcg aattcctgac
     8881 ttacttcctg tgttacttcc tgtattacct cttgtcttac ctcttgtctt acctcttgtc
     8941 ttacctcctg tcttccttcc tgtcttcccc tctcttcaaa ccactgcgcc agcgtcatca
     9001 tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc gcttgttcag
     9061 tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc
     9121 cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat
     9181 gtttttgcag tagttcgaga atcgcaatcc gccgatgctg catgatttcg tcatccggtg
     9241 tgatggtgat atccaccagc gggaaagggt tgttgtaaac gcgtcgcgcc agctccggcg
     9301 agtaaaacat atcaaaccag cacattgaga gcggataagg tgtggcctcg ccctgataaa
     9361 acagaatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg
     9421 cagcaataga ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttgatgtt
     9481 caatcaccac atgcaaataa ccgggattac cctgcatttg cacggaatag agcacgtcag
     9541 tgctgtgccc tttcaggctt tcttcaataa aactccccga ctcaagatgc agcgtgtcga
     9601 ggtcacacag ttcgcgtagt tccgctggca aatggatatc cagaaagtca cgagccgttt
     9661 ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca
     9721 tcttccctgc cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgcgccgtcg
     9781 tcagccgcct ctttattgtt taccgagcag cgttcagaga aatttcaatt ggttacaggc
     9841 gttacatggc acgcttcggc acggtacgca aactgctgat gcaaatgccg tcagatgagt
     9901 agacactaaa caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcatcgcct
     9961 gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa
    10021 actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga
    10081 ccaggatttt cttgacgcag ccggtattct cgaaaacgaa gccattgata tctggaatgt
    10141 caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat
    10201 ttccgttaac ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca tcatcgccag
    10261 cttcgttacc atgccagatg aagaagctcg cacctggcgg cccaacgtcg cctattttga
    10321 aggcgacaac gaaatgaaac gtaccgcgaa agccattccg gtacaggttg cctgataatc
    10381 acccctgcgg ctggttactc accagccgcg acattgtctc cagcgaatcc gttcttaaga
    10441 tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg
    10501 tcaccgccag gtgaaatccg gcttcttttg ctgccttcac ggctttgtca ttaaatccgc
    10561 caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac
    10621 gtgcaaaatc aaacaggata ttgtgctcac tacggctcag taatatgggt cggcgataac
    10681 catccacccg atgcaaaaaa tgggtatgtg actggaaatc aaatacatcg cgaatttcgt
    10741 taagttcaga aacgctcata aattgcagcg attttgggtc ccacttctgc gggtgacgtt
    10801 tgatgcgcga ggtaacaata aacgccgtcg ccttcatgcc atattgtttc aacacaggat
    10861 acgcatagcg gctcaccgac tttaggccat catcaaaggt aatcaccacc gctcgcgcag
    10921 ggagattgat cttattcttc acgtaacctt ccagttgcgc catgctcagt gtcacgtatc
    10981 ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag
    11041 tatggcgaaa acgggtattt tcttcgtcgc gcagaatatg gtgataggtt agcaccggca
    11101 ggccattatc gggttgggca tccagcgcgc tgatataggc cagtcgatcg ccaatacgga
    11161 tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaattggg tagcgcaaat
    11221 tgtccgccag taccccaaac ggcgcactgc ccgcactcgg cgcgttgaag accggcgtat
    11281 ctttccaggt tattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccatctt
    11341 caactttttg tcgcccctga accggctcga gatgaccttt atcgataaac cctttaccaa
    11401 agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgtccgg
    11461 cccgaatatt ccccaccgtc accatcttgt cgccaatttg cgcccagacc gcagcatttt
    11521 cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca
    11581 gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt
    11641 attgtagcaa aagcgtcatc agaatcgcgg gttaatactc atacaaatta tgtttgagga
    11701 cgaaaggcgg atttttttgt tgctggtgcc aaagactact cacctccggc gcacccagtt
    11761 cgacaatacg ctcgcgaaac tctgcgctgg ataacgtttc acggtagacc agcatctgtt
    11821 caattaacgg taacgtgacg ccagaaatca cttcgcaacg ggagtgtttg tggcttaata
    11881 atgaagccac gcgatacggt ggcgctccgg ctatatcggt taagaagatg acaccctcac
    11941 cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaatc
    12001 cgcgccaaaa attcacggcc cggcactgaa gaagcacccc atgttttttc tccagcgcat
    12061 ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc
    12121 tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa
    12181 ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg
    12241 ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa
    12301 ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat
    12361 ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga
    12421 gtaaaacacc ccgcccaaat acgtaagtgg cgttaacaca aaggttggca ccaggctgat
    12481 gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc gcaagggaga acaacaccgc
    12541 cgtgagcacc agcgttaaag cgacaaatac ccacgaatgc acctgaaacg gcacaaaaaa
    12601 cagtgaaatt gccgtgacca gaatgccaac aaacagacca cgcgccacgc caccgccgac
    12661 atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg
    12721 ctggaacttg gcaccaaaaa atgatgacgc gacgttagcg taggcgttgg taatcaccga
    12781 catcatgatc agccccggta caatgaactg catgtagctg aagccatgca tatcgccaat
    12841 acgcgaacca atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg
    12901 caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt
    12961 tagtgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa
    13021 acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc
    13081 tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagcacttc aacttccagc
    13141 gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg gcttttcggt
    13201 gcgagatcga gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaagta
    13261 ttttctacca gctcaccgtg ctgaataatg ccgatattgc ggcacagcat ttctgcttct
    13321 tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag
    13381 ccccacattg agcggcgaag ttcgatatcc acgcctgcgg tcggttcgtc gagaatcagt
    13441 agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat
    13501 aacatgcgcg cacgttcgtt gcgtttcccc catagatcga gctgtttaag atacttttcg
    13561 ctgcggatgt acgcttcttt gcgctccaca ccgtagtacc ctgcctgatt caccacaatt
    13621 tgctgtacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt
    13681 ttagcgttca cgacatcctt ctcgagatcg taaccaaata cgctgacccg cccggaggtt
    13741 ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg
    13801 agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag cgcctgaacg
    13861 ccgcctggat aggttttttt aagctgttga agttccagtg caatggtcat aaatttttac
    13921 ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta
    13981 gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata gatacactca
    14041 tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga
    14101 aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc
    14161 ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta
    14221 acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac
    14281 tcgaagttga acacattatt atctgtggtc actacggttg cggcggcgta caagccgcag
    14341 ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt
    14401 tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg
    14461 aactgaacgt catggaacag gtgtataacc tgggccactc caccattatg caatcagcgt
    14521 ggaaacgcgg gcagaaagtc accattcacg gctgggccta cggcattcac gacggcttgc
    14581 tgcgtgatct ggatgtcaca gcgaccaacc gcgaaactct tgagcaacgt taccgtcacg
    14641 ggatttccaa tctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca
    14701 acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag
    14761 atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga
    14821 gaaaccgatg aattctaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt
    14881 acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt
    14941 attacccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc
    15001 cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc
    15061 ggtcataaaa tcgacttcat gagatacctg aacttcacga cacaggtccg ccataaacat
    15121 aaatgagcca cgcagcagac ccaccagcac catatcgctg ccgctgtctt tgtaacgctc
    15181 agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac
    15241 ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg
    15301 caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc
    15361 gcggaatctg ttaataaagc tcgaattgtg atgacgatca cacatgttaa aacctataat
    15421 taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac
    15481 aggctcgcga cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta
    15541 tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc
    15601 tggccttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgcg ccgcgctttg
    15661 gctatacgcc gccctgctgc tcggcaccat gatttggggc gtctgggaag ttggtttcga
    15721 cttctgggcg ctgactccgc gcagcgacat tctggtcttc ttcggcatct ggctgatcct
    15781 gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg ctctggtggt
    15841 ggcgttgctg attagtggcg gaatcctcac ctgggcggga tttaacgatc cgcaggaaat
    15901 caacggcacc ttaagcgccg atgccacacc tgccgaagca atctcccccg ttgccgatca
    15961 ggactggcct gcctatggtc gtaatcagga aggtcaacgc ttttcacctc tgaaacaaat
    16021 taacgccgat aacgttcaca agctgaaaga agcctgggtg ttccgtactg gcgatgtgaa
    16081 gcagccgaac gatccgggtg aaatcaccaa tgaagtgacg ccaattaaag tgggcgacac
    16141 cctttacctg tgtaccgcgc accagcgcct gtttgcgctc gatgccgcca gcggtaaaga
    16201 gaaatggcat tacgatcctg agctgaaaac caacgagtcc ttccagcatg taacctgtcg
    16261 tggcgtctct tatcatgaag ccaaagcaga aactgcctcg ccagaagtga tggcggattg
    16321 cccgcgtcgt atcattcttc cggtgaatga tggtcgtctg attgcgatta acgctgaaaa
    16381 cggcaagctg tgcgaaacct tcgctaataa aggcgtgctc aatctgcaaa gcaatatgcc
    16441 ggacaccaag ccgggtctgt atgagccgac ttcgccgccg attatcaccg ataaaaccat
    16501 cgtgatggcc ggttcagtca ccgacaactt ctcaacccgc gaaacgtctg gcgtgatccg
    16561 tggttttgat gtcaacaccg gggagctgtt gtgggctttt gatcctggtg cgaaagatcc
    16621 taacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact cgtgggcacc
    16681 agcggcctat gatgcgaagc tggatctggt gtatctgccg atgggcgtga ccacgccgga
    16741 tatctggggc ggtaatcgca caccggaaca ggaacgttat gccagctcga ttctggcgct
    16801 gaatgccact accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga
    16861 tatggatctt ccggcacagc cgacgctggc ggacatcacc gttaacggtc aaaaggttcc
    16921 ggtcatttat gctccagcga aaaccggcaa catttttgtg ctcgatcgtc gtaatggtga
    16981 actggtggtt ccggccccgg aaaaaccggt tccccaaggt gcagcgaaag gcgattacgt
    17041 caccccgacc caaccgttct ctgaactgag cttccgtccg acgaaagatt taagcggtgc
    17101 ggatatgtgg ggagccacca tgtttgacca gctggtgtgc cgcgtgatgt tccaccagat
    17161 gcgctatgaa ggcattttca ccccgccatc tgaacagggt acgctggtct tccctggtaa
    17221 cctggggatg ttcgaatggg gcgggatttc cgttgatccg aatcgtgaag tggcaattgc
    17281 caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtcctg gtaacccgat
    17341 ggagcagccg aaagacgcca aaggcacggg tacggaatcc ggcattcagc cgcagtacgg
    17401 tgtaccgtat ggcgtcacgc tcaatccgtt cctctcacct tttggtctgc cgtgtaaaca
    17461 gccagcatgg ggctatattt cggcgctgga tctgaaaacc aacgaagtgg tgtggaagaa
    17521 acgcattggt actccgcagg acagtatgcc gttcccgatg ccggttccgg tgccgttcaa
    17581 tatggggatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc
    17641 cgctacggca gacaactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca
    17701 gggtcgttta ccagcgggtg gtcaggctac gccaatgacc tatgaagtga atggtaagca
    17761 gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat
    17821 tgtggcttat gcgctgccgg atgatgtgaa gtaaccataa tgcccggaga gatccgggca
    17881 tatttccgaa tacggtcttt ttataccgta aaccctaaca tcatccccgt atcttcatgc
    17941 tccagcagat ggcagtgcgc catataagca tgttctttcg gtgcctcgtg attaaacttc
    18001 accagcactt cgctgacatt accttctacc ttaacggtat ctttccagcc cgcgcgatga
    18061 gtcgctggcg gtttgccatt ttctgacaag atacggaact gcgtaccgtg gatatggaac
    18121 ggatgcagca tcatgtcgcc cacgccagag ataacccaac gttcgtattg ccctttcgcc
    18181 gccgcaaaca tcggcttgtt catatcaaac gcctgaccgt tgattttatt ggcatggtgg
    18241 aaatcgaact tcccgccgtg gttcatatga ttcatattgc cgtgccccat atggcccatc
    18301 atctggctgt gatccattcc ggccatcgcc tgatcgccat atttctccat tagcatctgc
    18361 atccccatca tatcgagcat cgggtccata gaaagttgca gcttgcgtac cgtcagccct
    18421 tccagcgaag gtaacgcagg caggctactt aatgtgtctg gcaaagcacc ggaggcacta
    18481 atagcaatcg gctgaatccg cattaccgga tgcggcttgt caaacggtgc aatcgccatc
    18541 cccatctggc tgaccggcag cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc
    18601 agcacttcaa aacgctcgcc catcagcacc ggcagttcgc tcaccttcac cggttcaggt
    18661 agcagaccac cgtcgctggc aatcacataa agcgggcgat tgtcgctggt ggcgaaattg
    18721 agcgagcggg cgttacagcc attgagcaaa cgcaggcgca gccaaccacg cggggcagcg
    18781 tgttgtgggt agattgcacc gttggtcagc aacgtatcgc caaaccagcc cacggcggcg
    18841 gtcatcacat ccagttgata atcaatctgc ccgtcagcgc taaatttctt atcctgaacg
    18901 atcaccggaa catcatcgat accccactgt tttggcagca ttaatttcag gatctcgtca
    18961 tcttcaatca ccaccaaacc agccagcccc atcgccacct gacgtccggt cttgccgtgc
    19021 tgatgcggat ggaaccagca ggtagcggca ggttgatcaa cgttcaacgt caccgagcgc
    19081 ttgccacctg gcggaataat tccctgcggg ccgccgtcca cttcacccgg tacttccagc
    19141 ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat agatatcaac cgttactgct
    19201 ttgccgcgct gtaatttcac cgccggcccc agcagattgc cgttatagcc ccaggtagtt
    19261 gcggttttcc cgctaaaggt ggactggcct gcgccaatag ttaactgaat gcgattacgg
    19321 gcatcggtcg tgagcaaatc agggatcggt aacgttgggc gttctgccgc aaatactgcg
    19381 cggctccaca gcggcaaagc cgaagccacg cccagcgcga cggaatattt taagaaatca
    19441 cgacgttgca tagttatttc cttattctta agcaggtgaa acaaaatcaa acgttgagca
    19501 tagtccttcc ccttacggga aggtcaagcc gcagacacaa tttaatcgac aataatggtc
    19561 gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa gcccctggta gaagcaatga
    19621 agacgttttt cagaacagtg ttattcggca gcctgatggc cgtctgcgca aacagttacg
    19681 cgctcagcga gtctgaagcc gaagatatgg ccgatttaac ggcagttttt gtctttctga
    19741 agaacgattg tggttaccag aacttaccta acgggcaaat tcgtcgcgca ctggtctttt
    19801 tcgcccagca aaaccagtgg gatctcagta attacgacac cttcgacatg aaagccctcg
    19861 gtgaagacag ctaccgcgat ctcagcggca ttggcattcc cgtcgctaaa aagtgcaaag
    19921 ccctggctcg cgattcctta agcctgcttg cctacgtcaa ataatccctg ataccttttt
    19981 gtagaaatat tgaccgtgca tccgcggtca atgttagcta ttatgttgcg cccttttttt
    20041 acgggtgtta acaaaggagg tatcaaccca tggccgaaaa aaaacagtgg catgaaacgc
    20101 tacacgacca gtttgggcag tactttgcgg tagataacgt tctgtatcat gaaaagaccg
    20161 atcaccagga tctgatcatt tttgagaacg ccgcatttgg ccgcgtaatg gcgctggatg
    20221 gcgtagtaca aaccaccgag cgcgacgagt ttatctatca tgagatgatg acccatgttc
    20281 cgctactggc ccacggtcac gcgaaacatg tgctgattat cggcggcggc gacggtgcca
    20341 tgctgcgtga agtaacccga cataaaaacg ttgagtcaat cacgatggtg gaaatcgatg
    20401 cgggtgtcgt gtcgttctgc cgtcagtatc tacccaacca taacgccggt agctacgacg
    20461 atccgcgctt taaactggtg atcgacgatg gcgtcaattt cgttaatcaa accagccaga
    20521 cctttgatgt cattatctcc gactgcaccg atcctatcgg tcccggcgaa agtctgttca
    20581 cctcagcatt ttatgaaggc tgcaaacgtt gcctgaatcc tggcggtatc ttcgtcgcac
    20641 aaaacggcgt ctgcttttta cagcaggaag aggctatcga cagtcatcgc aaactcagcc
    20701 attacttcag cgacgttggc ttttatcagg cggcgatccc gacctattac ggcggcatca
    20761 tgacctttgc atgggcgaca gataacgacg ccttacgcca tctctcaacc gaaattattc
    20821 aggcgcgttt tctcgcttct ggcctgaaat gccgttatta caatccggca gtccatacgg
    20881 cagcttttgc cttaccccag tatctgcaag acgcactggc ttcacagccg tcctaaggag
    20941 aagataagaa attgaaaaaa ctgaaactgc atggctttaa taatctgacc aaaagtctga
    21001 gtttttgtat ttacgatatc tgctacgcca aaactgccga agaacgcgac ggttatattg
    21061 cttatatcga tgaactctat aatgccaacc gtctgaccga aatcctgtca gaaacctgtt
    21121 ccattatcgg ggccaatatt cttaacatcg cccgccagga ttacgaacca cagggtgcca
    21181 gcgtcactat tctggtgagt gaagaaccgg tcgatccgaa acttatcgac aaaacagaac
    21241 accccggacc actgccagaa acggtcgttg cccatctcga taaaagtcat atttgcgtac
    21301 atacctaccc ggaaagtcat cctgaaggtg gtttatgtac cttccgcgcc gatattgaag
    21361 tctctacctg cggcgtgatt tctccgctga aggcgctgaa ttacctgatc caccagcttg
    21421 agtccgatat cgtaaccatt gattatcgcg tgcgcggttt tactcgcgac attaacggta
    21481 tgaagcactt tatcgaccat gagattaatt cgattcagaa ctttatgtct gacgatatga
    21541 aggcgctgta tgacatggtg gatgtaaacg tctatcagga aaatatcttc cataccaaga
    21601 tgttgcttaa agagttcgac cttaagcact acatgttcca caccaaaccg gaagatttaa
    21661 ccgacagcga gcgccaggaa attaccgctg cgctgtggaa agaaatgcgc gagatttatt
    21721 acgggcgcaa tatgccagct gtttaacggc tctggcggag ctcccaggct ccgccagatt
    21781 tatttacttc tgctgcacga aattgcggta agccgccacg acctgcagaa aatcctcaac
    21841 gccgcatagc gacaggcttt cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc
    21901 agcgaattcc agttgattgg cgcgaaccat cacctcttca ccgtccatcc acagggtgta
    21961 ttcatgccct gcccgttgcc aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc
    22021 cacttcatca agcaaggcca ggttttcttt cacctcttca ttaaaccagt gcccgaccac
    22081 ttcatgcccc atggacatac gcacctttac cactccggta atatcacgca gaaattcgta
    22141 atccatagtg tgttcctctg ctcccggcaa tgcgccatta ctgcattgca ccttaggttt
    22201 aattatcgca gcactatgca agattaaaag gtggaaaaga ataaggaata aaaataaacc
    22261 ccctttcctt gagcagaaag ggggtttaaa gaagtgttac ttgtcaggta ttcaggagaa
    22321 cacctgagct attttcaggc tgttacaccg cagtctggaa aatcaccccg tccgcttttt
    22381 cggtgtactg agaaagctgg ttgaagttca ggtaacggta agtatcaacg gcagttttat
    22441 ccacctgtgc cacgtaggtc tggtactctt ccggcgtcgg tagtttgcca atcagcgccg
    22501 caacagcagc cagttccgca gaagccagga agacattcgc gccagtaccc agacggttcg
    22561 ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac
    22621 ccatacacag ggaacagcca gggatctcga tacgcgcacc gctcttaccg aagacgctgt
    22681 agtagccttc ttcggtcaac tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc
    22741 gggtcggcaa ctggccttta tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga
    22801 tgttggtcat gcaggaaccg ataaacactt cgtcgatctt ctcaccctgt accgcagaca
    22861 gcggacgtgc gtcatccgga tcgttcggag cacacaggat tggctcttta atatccgcca
    22921 gatcgatgtc gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat
    22981 tcgccagcca tttttccatg ccctgaatac gacgttccag ggtacgacga tcgccgtaac
    23041 cttccgcgat catccacttc agcaggacga tgttggagtt caggtattcg atgatcggtt
    23101 ctttgttcag cttgatagta caaccagcgg cagaacgctc ggcggacgca tcggtcagct
    23161 caaaggcctg ctcaactttc agatccggca gaccttcaat ttccagaatg cgaccagaga
    23221 agatgttttt cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg
    23281 gaatagcgtg taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca
    23341 ccagaacgga ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca
    23401 gaccagaacc cgccgggaaa gaaatgccga tcgggaaacg ggtatgggag tcaccaccgg
    23461 taccgacggt atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg
    23521 gacgcagcga cacaccacca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca
    23581 cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg
    23641 agaagcccag gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct
    23701 gggaacctac agaagtcatt ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc
    23761 cgcaggcacg acctaccatt ttctgcgcca gcgagaagcc gcggtcgctc tcagcgacat
    23821 ctttcgcctg acggaacaca tcgctgtgcg gcagaccaag tgcttcacgc gctttggtgg
    23881 tcaggccacg cccgataatc agcggaatac gaccaccggc acgcacttca tcaatcagca
    23941 cgtcggtttt cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac
    24001 ctttgtacgg gtaaacgtca attacgtcac ccatgttcag gttagagacg tccacttcaa
    24061 ttggcagtgc acccgcatct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga
    24121 ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca
    24181 gaacggagtt cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg
    24241 ccagcgggaa acctttctgt tgcagagctt caatctgttt gatcggacca acaacaccag
    24301 gctgatctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga
    24361 tatccgggcg tgaccacgca tccggtgccg gagaaaggtc atcggtgttg gtttcgcctg
    24421 tgactttgaa gacggtaacg gtcagttttt cagccagtgc cgggcgattc aggaaccatt
    24481 cggcatccgc ccaggactgc attacctgct tcgcatattc gttgcctgct ttggctttct
    24541 cttctacgtc atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcga
    24601 tcggtgccag tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct
    24661 gcatagtgcc cagcagttcg atggcttttt ccggagtcag cagtggggat ttggcttcgc
    24721 ctttcgcgac agcagccagg aagcctgctt tgacataggc ggcttcatcg acgccagggg
    24781 gaacacggtt ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca
    24841 gcagctctac aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag
    24901 cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca
    24961 ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta
    25021 aatgcccagt accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac
    25081 aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc
    25141 cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta
    25201 gtcaaaattt aaactaccgc ctctttatac tcggattctc agcacctgcg ggtggcagtt
    25261 cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc
    25321 ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc gcctctgccg ggctatatat
    25381 aaaaagccgc acagtggaag cgcctgtgga agcgcaatcg acacaacaga atgcgcctga
    25441 catctccgca gttacgcttc ctgcaacggt ttccgcacct cccgtaacac ccgccgtcgt
    25501 caaatctgca ttcagcactg cacaaataga tcaatgggtc gcgcccgtcg cgctgtatcc
    25561 cgacgcccta ctttcgcagg tgctgatggc atcaacctat ccgacaaacg ttgctcaagc
    25621 agtgcaatgg tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc
    25681 tgaccagccc tgggacgcca gcgtcaaatc actggtggcc tttccacagt tgatggcatt
    25741 gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca
    25801 ggacgtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg gctcgctgaa
    25861 gtcatcaact gaacaaaaga tcatcacgac aacgaaaaaa gtcgtaccgg taaatcagcc
    25921 cgccaacgcc cctgtcacac aatcaaatac cgtttctacg tccagccccg tcgtcgcaga
    25981 acctgcaccg accgtaataa ccattgagcc agccaatccg gatgtggttt atattccgaa
    26041 ctacaaccca aacgtggttt acggcagttg ggccaatacc gcttatccgc cagtttatct
    26101 gccaccacca gccggagaac cgtttgttga cagctttgtg cgcggattcg gctatagcat
    26161 gggcgttgct accacgtacg cgctattcag cagcatcgac tgggatgacg acgatcatga
    26221 ccatcatcat catgacgatg atgattatca ccaccacgat ggcggtcatc gtgacggtaa
    26281 tggctggcaa cacaacggcg acaacatcaa tatcgacgta aacaatttca accgtatcac
    26341 cggtgagcat cttactgata acaatatggc atggcggcac aatccaaact accgtgatgg
    26401 tgtgccctat catgatcagg atatggcaaa gcggtttcac caaaccgatg tcaacggcgg
    26461 catgagcgcc acgcagttac ctgctctatc gcgcgacagt cagcgtcagg cagcagcaag
    26521 tcagtttcag caacgaacac acactgcgcc agtcattacg cgagatactc aacgtcaggc
    26581 tgcggcacag cgatttaatg aagcggaaca ttatgggagc tatgacgact tccgcgagtt
    26641 cagccgtcgc caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc
    26701 ggcctctcct gagcagcgcc aggcagtccg cgagaaaatg cagactaacc cgcagatcca
    26761 gcagcgaaga gaggcagcgc gtgagcgtat tcagtccgcc tcgcctgagc agcgccaggc
    26821 ggttcgcgag aaaatgcaga ctaacccgca gaaccagcag cgaaaagagg cagcgcgtga
    26881 gcgtattcag tccgcatcac cagagcagcg ccaggtgttt aaggaaaaag tacagcagcg
    26941 cccactgaac caacagcaac gtgataacgc ccgccagcgt gttcaatcag catcacctga
    27001 gcaacgtcag gtttttcgga agaaagttca ggagagccgc ccgcaacgtc taaacgacag
    27061 taaccgtact gccaggctga ataacgagca acggtcagcg atacgggaac ggttgtctga
    27121 gcgtggagca aggagaatgg aaaggtaaaa tgcaggcgta aaaaaagcgg cgtggttagc
    27181 cgctttttta attgccggat gttccggcaa acgaaaaatt acttcttctt cgctttcggg
    27241 ttcggcaggt cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc
    27301 agagtcgggt gcgcatggat ggtcagtgcg atgtcttcag catcacaacc catttcgatt
    27361 gccaggccga tttcacccag cagctcgccg ccgttagtac cgacaatcgc accaccgatc
    27421 acacggtgag attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg
    27481 atcgcacgac cagaagcagc ccacgggaag gtggcagttt catagctgat gcctttctct
    27541 ttcgcttctt tctcggtcag acccacccat gcaacttctg gttcggtata ggcgatggac
    27601 gggataactt tcggatcgaa gtagtgtttc ttaccggcga taacttcagc ggcaacgtga
    27661 ccttcgtgaa cacctttgtg tgccagcatc ggctgaccga caatatcgcc gatagcaaag
    27721 atgtgcggta cgttagtgcg cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact
    27781 tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc
    27841 agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt tgccttccat cgtcacataa
    27901 ataccgtctt ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc
    27961 ttgctgatac gcttggtgaa gactttaacg atgtctttgt cagctgccgg gataacctgg
    28021 tcgaacattt caaccacgtc aatctgtgaa cccagcgcgt ggtatacggt ccccatttcc
    28081 agaccgatga taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc
    28141 gcgtcagtgg agtcccagat acgcggatct tcatgcggaa taaacggcag ttggatcggg
    28201 cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca cggttttgcc gttctcacct
    28261 tcaacttcca gggtgttagc cccggtaaat ttacccagac cgttgaccac tttgactttg
    28321 cggcctttcg ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccag
    28381 gtacgaatct tgtcaatatc ggttttcggt tcgccgaaga cgataccgtg ttcagcaagc
    28441 gctttggctt cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg
    28501 acgttcaggc aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct
    28561 aaatcagcgc aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc
    28621 tgagttttga tttcagtact catcatgacc tctatatatt tatctccggc gggtctgacg
    28681 ttttttttat gacgcccatt atccaccggg tcgttctatc cgtcggtatt ttacaaaatt
    28741 gttaacaatt tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata
    28801 cattcatgag attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc
    28861 agacggcgaa tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca
    28921 ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac
    28981 tctttaccat tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc
    29041 ggcgcgttca caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg
    29101 aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata
    29161 gtcatcagct cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga
    29221 acaaccagac cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc
    29281 agacgctgac cgtcttccga cagcgaactg ttgaagcgag gcatctgctc aagagctgca
    29341 gcaaccgctt tcatgatgaa gacaactggg gttatcttca catccagctt acctttcgcc
    29401 gcttcttcgt tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag
    29461 tgagtaacat gcgggatcat cacccagtta cggctcaggt tcgcaccaga aattttctgg
    29521 atacggccca gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac
    29581 ggcagcatgc ctgggatacc gccgccagtc gccgccggag ccgcttctgc acgtttgata
    29641 gcttctttca cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc
    29701 ttcactttcg ccaggttaac gccaaactcg cgtgccagac ggcggatcag cggagtcgcg
    29761 tgaacgtaag cgtcgttttc agcaaattca gatttgcctt ccgctttcgc agccggtgct
    29821 gctgccggag cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgcagga
    29881 gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc agttttcact
    29941 ttatcgccaa cgttgacttt cagttccttc acgacgcctg caaacggagc cgggacttcc
    30001 atagaagctt tatcgccttc tacggtgatc agtgactgtt cagcggcaac tttgtcgccc
    30061 actttcacca tcacttcggt cacttcaact tcgtcaccgc cgatatccgg aacgttaact
    30121 tctttcacgc cagctgctga tgcaggggcc gctgccggag ctgcttcctg tttagccgcc
    30181 ggagctgccg cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac
    30241 actttgtcac ccacgttcac tttgatctct ttcacggtgc ctgcaaacgg agccggaact
    30301 tccatagaag ccttgtcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg
    30361 ccaactttca ccaggatttc ggtcacttcg acttcgtcgc tgccgatatc cggaacgtta
    30421 acgtcttttg ccgccgcagc cgctggtgct gctgccggag ctgcttcttt cttctcttct
    30481 gcctgagcag gtgcagcgtc agctgcaccg tcggcggaat cgaaaatcat aatcagtgcg
    30541 ccggtctggg ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga
    30601 gatggtactt ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca
    30661 actttgtcgc ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc
    30721 ggtactttga tttcgatagc cattattctt ttacctctta agccagacgc gggttaactt
    30781 tatctgcatc gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc
    30841 cacgtttagc cagttcgccc agcgccgcaa ccacgacata agaagcatca acttcgaagt
    30901 ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcgat
    30961 agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag
    31021 atgccactgc cggagcgtca ttcatcacct gagcgatata cggaacgcgc ggtgtttcca
    31081 gcgggtgcag catgttccag cgttcacaat cctgaccatc acgcgccagc tcggtgaagg
    31141 aggtcacgct ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt
    31201 cacggacgtg acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt
    31261 caatggtttc gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg
    31321 ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt
    31381 caccgtacat acgctccaga ccgtcatgca tgatgacagc aacttcgtaa gcgtaagccg
    31441 ggtcgtaaga gatacagttc gggatagtca gcgactgaat gtggctgtga ccatcttcgt
    31501 gctgcagacc ttcgccgttc agggtggtac gaccggaagt accgccgatc aggaagccac
    31561 gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat acgctggaag ccgaacatcg
    31621 agtaatagat gtagaacgga atcatcggca gattgttggt gctgtaagag gtcgccgctg
    31681 ccagccagga acaccctgcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct
    31741 cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg
    31801 ggctgtaaat accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg
    31861 cgatgatcgg taccagacga tctttgatcg acttgttctt cagcatcacg ttcagagcac
    31921 gaacgaaagc gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaagt
    31981 cttgcaggct cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac
    32041 cgtgcagttt ctgacgctga gcgtgcagat aggtatgctc ttcagaacct tccgggaagg
    32101 tgatgtacgg cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga
    32161 tatgacgcac gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag
    32221 ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag gattaccgtc gctttgcctt
    32281 tggtttcctg cgctttcttg aatgcagcgt agattttctt cggatcgtga ccgccacggt
    32341 tcagcgccca gatctgctcg tcagtccagt ctgcaaccag agctgcggtt tccggatatt
    32401 taccgaagaa gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc
    32461 cgtcaacggt ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt
    32521 catcccaacg gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc
    32581 cttccagttc gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt
    32641 tacagttgat aacgaagacc aggttatcca gtttttcacg ggtagcgatg gtgatcgcac
    32701 ctttggattc cggttcgtcc atttcaccgt cgccgaggaa ggcgtaaacg gtctgtttag
    32761 aggtatcttt caggccacgg tgttccaaat atttcaggaa tttagcctgg taaatagcac
    32821 caatcggacc cagacccata gatacggtcg ggaactgcca gaattccggc atcagtttcg
    32881 ggtgcggata ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct
    32941 cctgagtcag acgaccttcc aggaaagcac gagcgtaaac gcccggggag atgtggccct
    33001 ggaagtaaac cagatcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc
    33061 acacatcata aatggttgcg gaagactgga aggacgccat gtggccgccc agttcgaggt
    33121 cttttttcga cgcacgcaga accgtcatga tggcgttcca gcggatagct gaacgaatac
    33181 ggcgttccag ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga
    33241 tgtagttgct gataccggtg cctgcggcta cgttgacacc gcctttgcgg gcttcagcaa
    33301 gcagttggtc gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga
    33361 tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca
    33421 tgggttattc cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc
    33481 tcgccggaaa gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt
    33541 gatttacaac atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc
    33601 agacgacgca gagaacgctc acggcgactc tcttcgcggc ttctgtcgag caaaatttct
    33661 tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata
    33721 atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa
    33781 tagagcaatt cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc
    33841 agatgaagca gaaccacatt gtgggccgct tcggtgacgg caatctgata ctggagtacg
    33901 gcatttgatt ccgcgtccag atcgccagac tgctgcgcca gctctatggc gtggtggagt
    33961 tcacggatgc gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata
    34021 ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg attcaggatg gtcggagagc
    34081 agctccacca gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca
    34141 ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag
    34201 ggacgggaga cgtcgaactg tttcgccagt tcgcgttccg gtgggagttt ttcgcccggg
    34261 cggagtgtgc cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt
    34321 ggttggcgga ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat
    34381 tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat
    34441 ataggttttg attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt
    34501 ttcaaagttg taactatgca caaatgtaga ctttacgtag gaaaggggtt ttttaacgat
    34561 tattaaacta taaaaatccg aattgaaccg attcacttac caattttgtg atttttaatt
    34621 caattaaaac gaatttaaat tcattataca tattgagagg ggttgaggct gagctttaca
    34681 aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc
    34741 gcataccatt attcttgatc tgacggaagt ccttttgtaa caattcaaac ttctttgatg
    34801 taaacaaatc aatacaacaa acggaattga aaacttacac acgcatcact gcgtagatca
    34861 aaaaaacaat caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa
    34921 agcgcggcct taaaaaccgc cacatacagc ttatcgcgct gggaggcgcg atagggacag
    34981 ggctattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt
    35041 acgccattgc tggttttatc gcctttctga tcatgcgtca gctaggtgaa atggtggtcg
    35101 aggaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg
    35161 gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga
    35221 ctgccgtggg taaatacatt cagttctggt atccggaaat ccccacctgg gtttctgccg
    35281 ccgtgttctt tgtggtgatc aacgccatca acctgacaaa cgtaaaagtg tttggcgaga
    35341 tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg
    35401 gctggctgct attcagcggc aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc
    35461 agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg attatcatgt
    35521 tctctttcgg tggtctggaa ctggtgggca tcaccgcagc agaagcagat aacccggagc
    35581 aaagtattcc aaaagcgact aaccaggtta tctaccgcat cctgattttc tatattggtt
    35641 cgttagccgt tctgctctcg ctgatgccgt ggacccgcgt tactgctgat accagtccgt
    35701 ttgtactgat cttccacgaa ttgggcgata cctttgtggc taatgcgctg aacatcgtgg
    35761 tactgactgc tgcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt
    35821 ttggtctggc acaacagggc aacgcgccaa aagcgctggc gtctgtcgac aaacgcggtg
    35881 taccggttaa cactattctg gtgtctgcgc tggttacggc attgtgcgta ctgattaact
    35941 atcttgctcc ggaatccgca tttggcctgt taatggcgct ggtggtatct gcactggtaa
    36001 tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag
    36061 gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt
    36121 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
    36181 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
    36241 aagccgtaaa agcgcattaa tctctctacg ccctcacccg tgcagggtga gggcaataat
    36301 ctttacacca acgccccgta aatcgtcagt agcgcaatta ccaccacgac cacgaacgag
    36361 gttttcttcg ccatggaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca
    36421 cgcgccagag agaactgtgc cagacgcgtt aatacctgat attgcgaagt atggaaatca
    36481 cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa ggcatatacc
    36541 acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac
    36601 tgtaaacgat gatgcggcgt ctggtaacgc gccagccagt attgccatgc acgcaaaaac
    36661 gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt
    36721 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
    36781 cgttcatcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca
    36841 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
    36901 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagcagcgt gggaacgttg
    36961 aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
    37021 atgcctaatg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
    37081 tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
    37141 gttgtaaata gcgtcatgtt gtctccttgc tgaccagtgc acgaaaccgt gcccaatcaa
    37201 atgcaggacc gggatcggtt ttccgatccg gcgcgatatc acaatggccc gtcatgttat
    37261 tagcgatatc tggatagcga tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact
    37321 gcgcatcggt atacgccagc gtatcggtgc cttccagctc aatcccaata gaaaaatcat
    37381 tgcagcgttc gcgcccctga tactgagaga ctccggcatg ccaggcacgt ttatcgaaag
    37441 gaacatactg gactatttca ccatcgcggc gaatcaaaca gtgagcggag acgcgcaaat
    37501 gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt
    37561 cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg
    37621 tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa
    37681 ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt
    37741 tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc
    37801 tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat
    37861 ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa
    37921 tgacattacg gcaaaacttt taccggaaaa ctctcgctct catgccacag tgatcacccg
    37981 cgagaatggc gtattttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg
    38041 cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc
    38101 cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa
    38161 ttttgtgcaa accctttcag gggttgccag taaggtacgc cactatgtcg aattactgga
    38221 gggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagctct
    38281 gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt
    38341 tctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc
    38401 atcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga
    38461 tgaagccctg aaagcaggtg ccgatatcat catgctggat aacttcgaaa cagaacagat
    38521 gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac
    38581 tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct
    38641 aactaaacac gtacaggcac tcgacctatc aatgcgtttt tgctgattgc tgacagattt
    38701 atccgctcca tcgcgggcgg ataatctccc ctttcccaac tttcttcgta acgcctcgca
    38761 aatttcttgt ctttcgctgc aacataataa aaaactccgg aagctgcttt cccgtttcgt
    38821 tttttgcccc tcgcttgtgc tgatggcgtt tgccaaagta gcgccaacca aatcaaggag
    38881 cgaaacaaat ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca
    38941 tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg
    39001 cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc
    39061 tggaacatgg tggattagat acctgcgacg gtggcagtaa tggcatcccc tcgcctacca
    39121 ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag
    39181 aaagtctcaa tgggctaagc gtcgtcatga cgccgggttg ggataacgca aacggcgtca
    39241 ccggttggac gcgcaactgc aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag
    39301 atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcaccg
    39361 ccctgtgtct gcgttatcag ggagtcttgc tggatgccag cgaagaggtg gttcatgttg
    39421 cggtggtcga tgccccttcg catgagctac tagacgcatt gcatttcgct accaccaaac
    39481 gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac
    39541 agacattgcc cgtagctatt catgagaagc atcagcccaa agcagagttg ctgactcgaa
    39601 cgctacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg
    39661 cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggat gtttcaccgg
    39721 atgtcggagt cgcattaacc gccagattaa aagtgctggg caacctggat attgcggaac
    39781 atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc
    39841 gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg
    39901 tgagtcaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg gcggactttg
    39961 ctcatgcctt gcaacaacca cagggactgg tgctggtaac aggccctaca ggcagcggca
    40021 aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta
    40081 gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc
    40141 gtgccggact cacctttcag ggcgttttgc gtgcgttatt gcgccaggac cctgacgtca
    40201 ttatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa gcggcgcaaa
    40261 ccggtcacct ggtgttgtct accctacaca ccaattccac cactgaaacg ctggtacgtt
    40321 tacagcaaat gggagtcgcc cgctggatgc tctcatcagc gcttacactg gtaatagccc
    40381 agcgtctggt acgcaaactt tgcccacatt gtcgcctgca gcaaggggag tccatccata
    40441 ttccagacaa tgtatggtcg tcgccgctgc cccgctggca agcacccggt tgtgtacatt
    40501 gctaccacgg tttttatggt cgtacggcct tatttgaagt tctacccata acaccggtca
    40561 tacgccagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac gcccgacagg
    40621 cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct
    40681 ttgaagagtt aatccgcgta ttggggatgc cgcatggcga gtaagcaact ctggcgctgg
    40741 cgtggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtgct
    40801 ttgctgctta tggcactaca gcaacagatg gttaccccac taagcctgaa gcgaatcgcc
    40861 atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg
    40921 ctactcaaag ccgggttaac gctttctgaa gggctggcac tgctggcgga acagcatccc
    40981 agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct
    41041 ttttccaatg ccttattacc ctggtcagag gtatttccgc cgctctatca ggcgatgatc
    41101 cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa
    41161 aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tgcgttatcc catcatcatt
    41221 ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgtgctgcc ggagtttgct
    41281 gctatttata agaccttcaa caccccacta ccggtactaa cgcaggggat catgacgctg
    41341 gcagacttta gtggcgaatg gggctggctg ctggtgttat tcggctttct gctggcgata
    41401 gccaataagt tgctgatgcg ccgcccgacc tggcttatcg cgcggcagaa attgctgtta
    41461 cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ctttacgatt
    41521 ctggcgctga ctcaaagtgc aggcattact tttttacagg gcgtagagag cgtcagagaa
    41581 acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac
    41641 ggtcaaccca tctggctggc gctaaaaaat gccggtgagt ttagcccact ctgtttgcaa
    41701 ttagtgagaa caggagaggc atccggctcg ctggatctca tgttagacaa cctcgcccat
    41761 catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg
    41821 ttgctgatca taacgggagg aattatcggt acgctggtgg tggcaatgta tctgcccatt
    41881 ttccatttag gcgatgcgat gagtggaatg ggataacgct ggcgtgttgc tccacgccag
    41941 cgttgggaga ttacaggttg ttgaagatgc ggttttcttg ttccagcaca cggataaatg
    42001 tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca
    42061 ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg
    42121 ttttaccttc tgctgcgcgg tattccgcaa cgccgccaac gtgacgtttc atcgcagact
    42181 cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc
    42241 cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa
    42301 aggctttcgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc attccgccca
    42361 gaccgtgcgc agcatcggca cattcgatca ccgcagaaag ttgcggataa ccgacgcctg
    42421 ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcagcac
    42481 cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt
    42541 tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat
    42601 tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt
    42661 tttcgaaatc cgcatcagac gtgccagtag aaaccatcac atgtttcagc acatcagcgg
    42721 aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca
    42781 aaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg tccatatttg
    42841 cggctataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt
    42901 ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt
    42961 taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta
    43021 taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga
    43081 ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg
    43141 aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa tcatgatgaa
    43201 ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc
    43261 aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt
    43321 attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt
    43381 ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc
    43441 ttcgccaacc cggaagagaa aaactggctt aacgccctgc tgcatccgct gattcagcaa
    43501 gagacgcaac accagatcca gcaagcaact tccccctatg tactgtgggt tgtgccattg
    43561 ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgccagccca
    43621 gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa
    43681 atccttgctg ctcaggcacc gcgcgaagct cgccttgccg tggcagatga cgtcattgat
    43741 aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg
    43801 cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg
    43861 aacatccact aaatgaaaaa atgcgtacgt ggctgcgcat tgagtttttg attcagcaac
    43921 tcaccgttaa tttacccatc gctgaccacg ctggcgcact gcatttcttc cgtaatgtca
    43981 gtgaattact ggatgttttc gagcgtggcg aagtccgcac tgagctgttg aaagaacttg
    44041 accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta
    44101 ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta
    44161 tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc
    44221 caggcggctg ttgcagcttt gatttacctt cattgcacat ttggctgcat ctaccccagg
    44281 cgcagcgcga cagccaggta gaaacctgga ttgccagcct
//
LOCUS       DAYPHN010000020        41630 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000020 DAYPHN010000000
VERSION     DAYPHN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.020"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            267..1505
                     /gene="preT"
                     /locus_tag="ACG0BW_001105"
     CDS             267..1505
                     /gene="preT"
                     /locus_tag="ACG0BW_001105"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFK7258095.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            1499..2734
                     /gene="preA"
                     /locus_tag="ACG0BW_001106"
     CDS             1499..2734
                     /gene="preA"
                     /locus_tag="ACG0BW_001106"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFK7258096.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHSVTL"
     gene            complement(2805..3815)
                     /gene="mglC"
                     /locus_tag="ACG0BW_001107"
     CDS             complement(2805..3815)
                     /gene="mglC"
                     /locus_tag="ACG0BW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFK7258097.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(3831..5351)
                     /gene="mglA"
                     /locus_tag="ACG0BW_001108"
     CDS             complement(3831..5351)
                     /gene="mglA"
                     /locus_tag="ACG0BW_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFK7258098.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(5412..6410)
                     /gene="mglB"
                     /locus_tag="ACG0BW_001109"
     CDS             complement(5412..6410)
                     /gene="mglB"
                     /locus_tag="ACG0BW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFK7258099.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(6690..7730)
                     /gene="galS"
                     /locus_tag="ACG0BW_001110"
     CDS             complement(6690..7730)
                     /gene="galS"
                     /locus_tag="ACG0BW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFK7258100.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(7872..9029)
                     /gene="yeiB"
                     /locus_tag="ACG0BW_001111"
     CDS             complement(7872..9029)
                     /gene="yeiB"
                     /locus_tag="ACG0BW_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFK7258101.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAIGNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(9046..9714)
                     /gene="folE"
                     /locus_tag="ACG0BW_001112"
     CDS             complement(9046..9714)
                     /gene="folE"
                     /locus_tag="ACG0BW_001112"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFK7258102.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            9972..10808
                     /gene="yeiG"
                     /locus_tag="ACG0BW_001113"
     CDS             9972..10808
                     /gene="yeiG"
                     /locus_tag="ACG0BW_001113"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFK7258103.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(10840..12819)
                     /gene="cirA"
                     /locus_tag="ACG0BW_001114"
     CDS             complement(10840..12819)
                     /gene="cirA"
                     /locus_tag="ACG0BW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFK7258104.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGDTPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(13112..14581)
                     /gene="lysP"
                     /locus_tag="ACG0BW_001115"
     CDS             complement(13112..14581)
                     /gene="lysP"
                     /locus_tag="ACG0BW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFK7258105.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(14786..15667)
                     /gene="yieE"
                     /locus_tag="ACG0BW_001116"
     CDS             complement(14786..15667)
                     /gene="yieE"
                     /locus_tag="ACG0BW_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFK7258106.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAIEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15766..16815
                     /locus_tag="ACG0BW_001117"
     CDS             15766..16815
                     /locus_tag="ACG0BW_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFK7258107.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            16889..17746
                     /gene="nfo"
                     /locus_tag="ACG0BW_001118"
     CDS             16889..17746
                     /gene="nfo"
                     /locus_tag="ACG0BW_001118"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFK7258108.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            17749..18837
                     /locus_tag="ACG0BW_001119"
     CDS             17749..18837
                     /locus_tag="ACG0BW_001119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFK7258109.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTINCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18944..20194)
                     /locus_tag="ACG0BW_001120"
     CDS             complement(18944..20194)
                     /locus_tag="ACG0BW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFK7258110.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(20294..21235)
                     /gene="rihB"
                     /locus_tag="ACG0BW_001121"
     CDS             complement(20294..21235)
                     /gene="rihB"
                     /locus_tag="ACG0BW_001121"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFK7258111.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPVIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFGPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            21365..22063
                     /locus_tag="ACG0BW_001122"
     CDS             21365..22063
                     /locus_tag="ACG0BW_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFK7258112.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(22131..23381)
                     /locus_tag="ACG0BW_001123"
     CDS             complement(22131..23381)
                     /locus_tag="ACG0BW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFK7258113.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKGISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(23475..24413)
                     /gene="psuG"
                     /locus_tag="ACG0BW_001124"
     CDS             complement(23475..24413)
                     /gene="psuG"
                     /locus_tag="ACG0BW_001124"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFK7258114.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGSEGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(24401..25342)
                     /locus_tag="ACG0BW_001125"
     CDS             complement(24401..25342)
                     /locus_tag="ACG0BW_001125"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFK7258115.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKSWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVMSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(25766..27457)
                     /gene="fruA"
                     /locus_tag="ACG0BW_001126"
     CDS             complement(25766..27457)
                     /gene="fruA"
                     /locus_tag="ACG0BW_001126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFK7258116.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDNAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(27474..28412)
                     /gene="fruK"
                     /locus_tag="ACG0BW_001127"
     CDS             complement(27474..28412)
                     /gene="fruK"
                     /locus_tag="ACG0BW_001127"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFK7258117.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(28412..29542)
                     /gene="fruB"
                     /locus_tag="ACG0BW_001128"
     CDS             complement(28412..29542)
                     /gene="fruB"
                     /locus_tag="ACG0BW_001128"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFK7258118.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            29910..31091
                     /gene="setB"
                     /locus_tag="ACG0BW_001129"
     CDS             29910..31091
                     /gene="setB"
                     /locus_tag="ACG0BW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFK7258119.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(31088..31342)
                     /locus_tag="ACG0BW_001130"
     CDS             complement(31088..31342)
                     /locus_tag="ACG0BW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFK7258120.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEILTAP"
     gene            31497..32069
                     /gene="yeiP"
                     /locus_tag="ACG0BW_001131"
     CDS             31497..32069
                     /gene="yeiP"
                     /locus_tag="ACG0BW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFK7258121.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            32292..33758
                     /locus_tag="ACG0BW_001132"
     CDS             32292..33758
                     /locus_tag="ACG0BW_001132"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFK7258122.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHASRTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            33876..34862
                     /gene="yeiR"
                     /locus_tag="ACG0BW_001133"
     CDS             33876..34862
                     /gene="yeiR"
                     /locus_tag="ACG0BW_001133"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFK7258123.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHHHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            34901..35614
                     /gene="lpxT"
                     /locus_tag="ACG0BW_001134"
     CDS             34901..35614
                     /gene="lpxT"
                     /locus_tag="ACG0BW_001134"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFK7258124.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            36026..36592
                     /gene="mepS"
                     /locus_tag="ACG0BW_001135"
     CDS             36026..36592
                     /gene="mepS"
                     /locus_tag="ACG0BW_001135"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFK7258125.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            36773..38329
                     /locus_tag="ACG0BW_001136"
     CDS             36773..38329
                     /locus_tag="ACG0BW_001136"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFK7258126.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTEMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            38411..40225
                     /locus_tag="ACG0BW_001137"
     CDS             38411..40225
                     /locus_tag="ACG0BW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFK7258127.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGCYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSSITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKNHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            40226..41320
                     /locus_tag="ACG0BW_001138"
     CDS             40226..41320
                     /locus_tag="ACG0BW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFK7258128.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            41320..>41630
                     /locus_tag="ACG0BW_001139"
     CDS             41320..>41630
                     /locus_tag="ACG0BW_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416684.1"
                     /note="part of the microcin C transport system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7258129.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLEHNGWVLWAPIRFGA
                     T"
BASE COUNT        10341 a        10831 c        10217 g        10241 t
ORIGIN      
        1 gctgcggata taacattctg aaccgcagct atttgtgaat cctttcacag tttaaatttc
       61 cccacgcgca tagccttaat atcagcacgc aatatttata ctctaaataa ttcaagttgc
      121 aggaaggcga caagtgagtg agtccccagg agcttacata agtaagtgac tggggtgagc
      181 gaacgcagac gcagcacatg caacttgaag tatgacgagt atatttagcg ttcgccttaa
      241 ttactcatag cattaaggaa gatcacatgc cgcaacaaaa ttatctggat gaactcactc
      301 cggcttttac accgttactg gcgattaaag aagcctctcg ctgcttatta tgtcacgacg
      361 ctccctgtag tcaggcttgc ccggcacaga ccgatccggg gaaatttatt cgctcaatct
      421 actttcgtaa ttttaaaggc gctgccgaga caattcgtga aaataatgcc ctcggtgccg
      481 tttgtgccag agtgtgcccg acggagaaat tatgccaaag cggttgtacc cgtgccggtg
      541 ttgatactcc cattgatatc ggccgcttac agcgttttgt tactgatttt gaacaacaaa
      601 ctgggatgga aatttatcag cccggtacta aaacgctcgg caaagtcgcc attattggcg
      661 caggtcctgc cggattgcag gccagtgtga cgctgacaaa ccagggttat gacgtcacga
      721 tctatgagaa agaagcacaa cccggtggct ggttgcgtaa cggcattccg caattccggt
      781 taccgcaatc agtactggat gcagaaatcg cccgtattga aaaaatgggc gtgaccatta
      841 agtgcaataa cgaaatcggt aaaacactca ccctggagca gctgaaagcg gaaaaccgcg
      901 cggtactggt caccgtgggg ttatctagcg gttccgggct accactgttt gagcatagcg
      961 acgttgagat tgccgtcgac ttcttgcaac gtgcacgaca ggcgcaaggc gatatcagca
     1021 ttccacaaag cgcattaatt atcggcggcg gtgatgtcgc gatggacgta gccagcacgc
     1081 tgaaagttct cggctgtcag gcggtaactt gcgtagcgcg tgaagagtta gatgagtttc
     1141 cggcaagcga aaaagagttt gccagcgctc gggagctggg cgtttcgatc atcgatggat
     1201 tcacgccagt agccgtggaa ggcaataaag tcacgtttaa gcatgtacgg ttaccgggcg
     1261 aactgacgat ggcggcagat aaaattattc tcgccgtcgg tcagcatgcc agactggatg
     1321 cctttgcgga gttagagccg cagcgtaaca ccatcaaaac acaaaattat cagacccgcg
     1381 atccgcaagt ctttgctgct ggcgatattg ttgagggtga caaaaccgtg gtctatgcag
     1441 tgaaaaccgg gaaagaagcc gccggggcga ttcatcacta tttagaggga gcttgctcat
     1501 gttaacgaaa gatctttcga ttactttttg cggcgtgaag tttcccaacc cgttctgcct
     1561 ctcttcttcg ccggtaggca actgctatga gatgtgtgcc aaagcctacg acacaggctg
     1621 gggtggcgtg gtgtttaaaa cgatcggttt ttttatcgcc aacgaagtct cgccgcgttt
     1681 tgatcatctg gtgaaagaag ataccggttt tatcggcttc aaaaatatgg agcagattgc
     1741 tgaacatccg ttggaagaga atctggccgc cctgcgtcgg ctgaaggaag attacccgga
     1801 caaagtattg atcgcttcga tcatggggga aaatgagcag caatgggagg agctggcgcg
     1861 cctggtgcaa gaagctggcg cggatatgat cgagtgtaac ttctcctgtc cgcaaatgac
     1921 ttctcatgcg atgggtagcg atgtcgggca aagcccggag ctggtagaaa aatattgtcg
     1981 ggcagtaaaa cgtggttcca cgctgccaat gctggcgaag atgacgccga atatcggtga
     2041 tatgtgcgaa gtggcgctgg cggcgaagcg cggcggcgca gatggcattg cggcgattaa
     2101 caccgttaaa tcaatcacca atatcgatct taatcagaaa attggtatgc cgatcgttaa
     2161 cggtaaatcg agtatttccg gttattccgg taaagcagtg aaaccgatcg ccctgcgctt
     2221 tattcagcaa atgcgcaccc atccagaact gcgcgatttc ccgatcagcg gtatcggcgg
     2281 cattgaaacc tgggaggatg cggcggagtt tttattgctc ggcgcagcaa cgttacaggt
     2341 gaccaccggc attatgcagt acgggtatcg gatagtggaa gatatggcga gcgggttgtc
     2401 gcattatctc gccgatcagg gatttgattc gttgcaggag atggtaggtc tggcgaataa
     2461 caatattgtt ccggcggaag atttagaccg cagttatatt gtctatcccc gtatcaatct
     2521 cgataaatgt gttggctgtg gacgctgtta tatttcctgt tacgacggcg gtcaccaggc
     2581 gatggaatgg agcgagaaaa cacgcacgcc gcattgtaat accgagaaat gcgtgggttg
     2641 tctgctttgt ggtcacgtct gcccggtggg ttgtattgag cttggggaag tgaagtttaa
     2701 gaagggagag aaagaacact ctgtaacgtt gtaaaaccag cgacgcatcc ggcatttggc
     2761 accgattgcc ggatgcgacg catccggcaa tacccacaat cacatcactt cttacgcgcg
     2821 tatttcagtg aatccagcgc cacggcgaag ataataatcg cccctttgat gatgtactgc
     2881 cagtacgggt ttacaccgat gtacgtcagg ccatagttga tgacggtaaa aataattacc
     2941 ccggtcacca cgccaatcac cgtccccaca ccgccgctga acgatacgcc gcctaccacg
     3001 cacgccgcga tagcatccag ctcatacata aagccgaggt tgttggtggc agagccgata
     3061 cgtccggctt ctaacatccc gccgaaggca tagaacacac cagacaacgc gtagatcatc
     3121 agcaggttca ggccgacgtt gacaccagaa actttcgccg cttccgggtt accgccgata
     3181 gcaaaaatgt tcttgccgaa gcgggttttg ttccacaaca cccagacgaa cgccaccgca
     3241 atcagcgcgt agaaggtgat gtaagagaga cggaaactcc ccagcgcgac aaagccctga
     3301 gcaaaggtag agaagccact gtcaaaacca gaaattggcg acgccccgac aaagtcgtaa
     3361 tagagcgagt tgatgccata gacgatgatc atcgtgccga gcgtggtaat gaacggcgtc
     3421 acgttgagat aagcgataat caggccgttg atcaaaccga tcaccgcacc aatggcacag
     3481 acaatcagaa taaccagcgc aatcggcatc gtcgccattt ccgggaacac tttgttggcg
     3541 ttatccatgg actgcaataa cgtcgccgct actactgccg ccagccccac ctggcgacct
     3601 gcagaaaggt cggtcccctg ggtgacaatt aaccctgcca caccgagcgc gataataata
     3661 cgcaccgatg actgggtgag aatattactt aagttcaaca gacttaaaaa tgttgggtcc
     3721 tggaaaataa taattgccag caaaactaaa agaacgacgt aaataccgcc ctctttcagg
     3781 taagtaagaa aacttttctt atttaacgca ctcatgggaa gcccctaatc ttaaaggtgc
     3841 aaagacgcaa gacgcagaat ttcgttttgc gttgttgttt ttgtatcgac aattccagaa
     3901 acgagaccat tgctcatgac cagaatacgg tctgttatcc ctaacaactc tggcatttcg
     3961 gaggagataa taataatccc cttgcctttc ttcgccagct cggcaattaa ctgataaatc
     4021 tcaaacttcg ccccgacatc aataccgcgc gtcggttcat cgagcattaa tatttctggt
     4081 tgcgttaaca gccagcgacc aataatcacc ttttgctgat taccacctga gagcgaacca
     4141 atttgcgtcc gatgccccgg tgtttttacc cgcatcgaat caatcaccca ctgggtatcg
     4201 cttttcatcc gcgagttatc cagtaaacca actttatttt tgtagttgcg aatattggaa
     4261 attaacgagt taaaaccgat atccagatag gcataaattc ccgttgagcg gcgctcttca
     4321 gttaccagtg caaatccgtg gtttatggct tcgttggcat tatggttatt gatctttttg
     4381 ccgtgcaacg taatggtgcc agccgatttc tcgcgaatac caaataacgt ctcaacaata
     4441 tcggtacgtt tcgcccccac cagaccggca ataccgagga tctccccttt atgcagatca
     4501 aacgagacat cgcgaatcga cggctggcgc agtgacgtca ggttacgcac ttccagaatc
     4561 acttcgcccg gcttgttttc tttgtcaggg aaacgttggt taagagaacg cccaaccatc
     4621 atggcgatga tcttgtccat cgtcagccct gccagcggtt cggtagcgat ccactgaccg
     4681 tcgcgcaata cggtaacttc atcacataac tggaagattt cttccatttt atgcgagata
     4741 taaacaatac cgcagccgcg ctcttttaat ttacgaataa tagtgaacag atgattgacc
     4801 tctttttcgg tcaacgaaga agtcggttca tccataatca caattttcgc gttataggaa
     4861 aacgctttgg cgatttcgat catctgcatt tgcgaaacgg ataatgtgcc gacgcgcgca
     4921 cgcggatcga tatcaatatc cagttcatca aaaatcgctt tggtttcacg gtacattttg
     4981 tcctgatcga caaacatacc tttggtggga tatcgcccca gccacatgtt gtccatcacc
     5041 gaacgttgta ataccaggtt taactcctgg tgtaccatcg aaataccatt ttccagtgct
     5101 tctttggcag aatggaaatc gatctcttta ccctggaata aaatggtgcc ggagtctttt
     5161 tgataaatac caaacaggca ttttaataat gtcgattttc ctgcaccgtt ttcccccatt
     5221 aatgcatgga tagaatgtgg ccggactttt aaattaacgt tatcaagtgc cttaacacca
     5281 ggaaaggact tgttgatacc gctcatttcc aacaagtatt ccccggagga cggagtcgtt
     5341 gagctgacca tataatttta ccttgttggt catacaataa gggcgcagta atagactgcg
     5401 cccaatcagt cttatttctt gctgaattca gccaggttgt ctttatctac gccaacataa
     5461 ggtacgcgga ccactttgtt gtcgattttc cagttggtgc catcagccgc acctttgcca
     5521 tcggccaggt ttttcgccaa atcaaaggtc gctttcgcct ggttgttagc atcgttcagt
     5581 acggtgcccg ccagtgcacc ggatttcacc agcgccagcg cttctggcag cgcatcgacg
     5641 ccaaacaccg gaatgctgga cttgttgtgt gctttaagtg cttctaccgc gcccattgcc
     5701 atcgcatcgt tgttggcgat aaccacttcg attttgttgg cgttcgggcc agacagccag
     5761 gcgtccatct tatctttcgc ctgagcggta tcccacatag cggtatctaa ctgtaactgt
     5821 tcagttttga tgcctttgtc gttcaattct ttaatcacgt aagtggtacg tgcttctgca
     5881 tccggatggc ccggctcacc tttcagcagt acgaactgaa tctgaccgtc tttgttcaga
     5941 tcccacccct gattcgccgc ccagtgttta gcaatcaaat cgccctgaat aatgccggac
     6001 tctttggagt cagtgccaac gtagtaggct ttgtcgtagc tatccagcgc cttacgagac
     6061 ggttctttgt tgaagaaaac caccggcacg ttttgcccac gcgctttctc aatcaccgta
     6121 cccgcagctg ccgggtcaac caggttgatt gccagtgcct tcaccccttt cgccagcaat
     6181 acgtcgatct gatcgttctg cttggactgg tcattctgag aatcattcat cagcagctga
     6241 acatctggcg cggctttcgc atcttgctca atagccttgc gcaccacaga cataaagtta
     6301 tcgtcgtact tgtagattgt tacaccaatg cgagtatcag cagcgtgtgc agcggcaccg
     6361 aataacatgc tggccatcac agcagacagg gttaacacct tcttattcat ggtatctccg
     6421 gtttttctta tgcagggtag tgcttgagat aaatgctcgg cggggcagta gagttaatga
     6481 agtgttactg aacgccgaag ctcacttttt aaaattcgtt cttccatgcc cggtaacgct
     6541 ccagaaaacg gctttaattg ttgttatgac gctattacct cggtgaaagt gattattcac
     6601 tgttacatac gggttacaac gttaaaacgg tgcaatcata gctatcgcat tgttaagata
     6661 ctgtgaaatc actcacagat tgaaagcggt tacatcgcca gatttgttga gttagtgatc
     6721 gccgccgcat tctggcgtgt tgcgacagaa tggcgacgca ctaacgtcgg cataaaacag
     6781 tggctggcac gaggatcaat attgcctgct gccccctgca aggccagttc ggtggctaat
     6841 ttcgccattg aagcaatggg ataacgtacg gtcgttaatt gcgggtcggt gtaacgggcg
     6901 atgggaatat catcgaaacc gatgattgag agatgtaacg gaatcgcaat gccattatct
     6961 tttaatgctg tcagtgcgcc agcagccata ttgtcgttat aagcaaatac agcggtaagt
     7021 tgtagattgc gccccagcag ttcaaccatc gccgcctcac cgcccggcat gtccggcgta
     7081 ccagtgccaa tccagctttc cggcggaata atatcctgct ctttcaacgc actcatccag
     7141 cctgctttac gcatggcgtc atcttcaatg ccgtggctgg aagaaagata accaatacgt
     7201 tgatgaccgt tattcagcaa catgcgcgtc gccattcggg caccgctgag attatccagg
     7261 caaacgcaac gatgggcgta ccccggcaca acgcggttga ttaacaccat accgggaatg
     7321 ttatccataa attgcgccag ttcatcgtca ctcaatgctt ttgagtgaac aatcaatgca
     7381 ttacaacgct ggcgaattaa cacctcaatg gcgtgacgct ctttttccgc ttcatgatag
     7441 ctattgccga ttagcacgta tttctgatgc tgctgggcga ccagatccac cgcttttacc
     7501 agcgcgccga aaaacgcatc agaaacgtcc atcaccacca cgccaatggt gtcgctaacc
     7561 tgagttgcca gcgcctgggc attggcgttt ggtcgataat ccagctcact cacggctttc
     7621 attactgctt cacgcgtgtc ggcactgacc agcgtgctgt tattgagcac tcgggaaacc
     7681 gttgccacag agacgccagc ctgacgcgct acatcacgaa tggtgatcat attcactacc
     7741 tgttatgaga ttgcagtaaa tgactgcttg ctggcggcta ttttgtcagc actgaataca
     7801 ggacttcgtg aatcgagtca cagcaatgga aacggttaca gccattttgt taacgaatgt
     7861 gatccagatc gttatcttga tgttttagat attgccggtc cggcagcacg caaagttaac
     7921 tgacgccaga gccattccac cggcccctgg cggaagtaac gcagccagat aacagagaag
     7981 aggatattcg ccagccatac cggaataaca aacgccagca gctccagacg gtcaaaatgc
     8041 ataaacaaac cgaggtggta aaaaagcgtg gtacaaatca gcgtttgcaa tagatagttg
     8101 gtcagcgcca tccgtccgac gcaggcgatc gcaagcacca gcttaaagcg gcttaattgc
     8161 ggccagaagc cataaaacag cgacgcatag ccaatcgcct gaaacggcgc actcagttcc
     8221 cgcggcattt gaagtaagaa ggcgcaccag cgatacgccc agtccagccg ccattgcagg
     8281 gcgatagcag gaaggttaat gatcacccca atcgccacca gcacaaaacc agtacgacga
     8341 tagtgacgta agctgaactg ccctttcagc cagccgctgc gcatcagcgc cgcaccaatg
     8401 agcatcatcc ctgccagttg ccagccatac tgtgcgccca gtgccagtaa actgttgcca
     8461 acaccatcgg cacgattacc gatcgcttca acgccgccgt gaagcttcca gtatttttca
     8521 tacaaaatag ccgatgcatc aggtgtccag gcgcggctgg tctggctatc ggaaatcaac
     8581 cccaataaca gcaaaacgcc aagccccacc agataaagca tgacgcctgt attaaacagg
     8641 ctttttaccg atggcgcatc gcgcaccagc cgccagcaga ttaagcccac cagcccgtaa
     8701 gccagcagaa tatcgccgtc ccagaacaat aaaccgtgaa taaagcccaa caagactaac
     8761 agcgttaacc gcgactggat ccagcgtctg ccacggggca gcaacatttg caggccagca
     8821 ccaaacagca gcgcaaaaag cgtgaggaat ttcacctggc cgatgagatc gagaaatgcc
     8881 caggtccatg catcctgcgg cgtaatagca ccgtaccagg cgggattgag ataagccgcc
     8941 tttggtagcc caaaggcgct gatgtttaat agcaggatcc ccagaatggc gacgccgcga
     9001 acaaaatcga gcgtgacgtt gcgctccatg gttcctgcct tttaatcagt tgtgatgacg
     9061 cacagcgcgc agaaactcgt ggcgcgtatt ctgactggat ttgaacaatc caccaagaga
     9121 ggtcgttgtc gtggcactgg ttgcatcgcg gatgccacgc gccttcacgc agtaatgcac
     9181 cgcgtcgata gaaacagcca cgttattggt gcccaacagc gtttgtagcg caatgagaat
     9241 ttgctgcgtc agacgttcct gcacctgcgg acgctgggca aagaactgca cgatacggtt
     9301 aatttttgac agaccgatca ccgaatcttt cgggatataa gccaccgtcg ctttgccatc
     9361 gatggtaaca aaatggtgtt cacaggtact ggtcagagtg atatcgcgca cggtgaccat
     9421 ttcatcgacc ttcattttgt tttcaatgag ggtgattttc gggaaattgg cgtaatccag
     9481 accggagaaa atttcatcga catacatttt agcgatgcga tgcggcgttt ccatcaaact
     9541 gtcatcagcc aggtcgagat tcagcagctg catgatttcg gtcatatgac cagcaataag
     9601 gcttttgcgc gtttcgttat ccatttcatg cacgggcggg cgcagcggtg tttccagtcc
     9661 tcgcgcaact aacgcttcat gaaccagggc cgcttcttta ctgagtgatg gcatttatga
     9721 tttctcctgc aggtgtgacg cctccgccct gcgtgggggc aaagttatta agctgattta
     9781 cagcctgatt attgtgcgtg aggcggcgca cataatccag tattcacagc gataattatt
     9841 gtaattgccg ctgcctttca tcagcagatg ttaaaacatc gttatgcaaa tacggaagtg
     9901 aaagttactc acagcacatt gaataaacgg tatgatgaag aaattgcaaa caacacaaca
     9961 aggagccacg catggaaatg ctcgaagagc accgctgttt tgaaggctgg cagcaacgct
    10021 ggcgacacga ctccagtacc ttaaactgtc cgatgacgtt cagtatcttt ctcccaccac
    10081 ctcgtgatca caccccgcct ccagtgctgt actggctttc cggattaacc tgcaatgacg
    10141 agaacttcac caccaaggcg ggtgcccagc gcgtggcggc agaactgggg attgtactgg
    10201 tgatgccaga caccagcccg cgcggcgaac aggttgccaa cgacgatggc tacgatttag
    10261 gccagggcgc aggcttttat cttaatgcca cgcaaccgcc gtgggcgacg cattaccgga
    10321 tgtatgatta tctgcgcgat gaattaccgg cgctggttca gtcgcaattt aatgtcagcg
    10381 accgctgcgc cattagcggt cattcaatgg gtggtcacgg tgcgctgatt atggcgctga
    10441 aaaacccggg caaatacacc agcgtttccg cctttgcgcc aattgtgaat ccgtgcagcg
    10501 tcccgtgggg aatcaaagcg tttagccgct atttaggtga ggacaaaaat gcatggctgg
    10561 aatgggacag ttgcgcgctg atgtatgcca gtaacgcgca ggatgcgatc ccgacgctta
    10621 tcgatcaggg cgataatgat cagtttcttg ctgaccaact gcaacctgca gtacttgcag
    10681 aagccgcgcg ccagaaagca tggccgatga cgctgcgtat tcagccggga tatgatcaca
    10741 gttactactt catcgcctct tttatagagg atcacctgcg cttccatgcg cagtatttac
    10801 tgaagtgaaa gtccgcccgg ttcgccgggc atcttctcat cagaagcgat aatccactgc
    10861 cataaagtaa cgacgtccgt cttcgttata gctgtagtcg tcacgactga gatccttgtc
    10921 gccaaggttc agcacgcctg cgcgcagttt gacgtcttta gtcacctgcc aggccgcgcc
    10981 ggtattccag atggtataac cgccaggtgt tttagccgtc gcgctgtcgg ctcgtttctg
    11041 cccggtatag tgaccagaaa catagaatga ccagtcttcc agcgccagcg gtttccagtc
    11101 cagcgtaccg ttagcggtat ggaacggcag atcggatagc ggtttgtttt cgccgttgct
    11161 gacatcacga ccatcgttgt aggtgtagtt gagtgacagt ttccattcat cgttgaacgg
    11221 aattttcagt tcggtttcca cgccctgaat acgagctttg ttaacgttgt agtagctaaa
    11281 taccggcaca ccgttcgctg taaaaccaac aaagttttgg tagcccggtg cagcattgac
    11341 gtcagacgta cggctgatgc tgatacgatc tttcacatcg ttacggaaaa cggtaacgct
    11401 ggattcaacc ccttccagcc agccttcttc acccatgtag taaagcccca gctcccaact
    11461 ttcgctggtt tctggtttca gatccgggct acccacaatc ttacatgcgc cacggcagga
    11521 attgctcgtc cagtcagggc taagttgcaa cagagaaggt gctttaaatg ccgtcgccca
    11581 gccccctttc accgttacgg tgtcggtggc gttataaacc aggtaggcac gcggactcca
    11641 gtgttcaccg taggtttcgt gatcgtccat acgcacgccg gtcgtcagcg ccagcggctc
    11701 gaagatccgc cattcatctt ccacaaacag cgcgtactgg ctggcagacg ttttggagct
    11761 ggttcccccg gtcaggttca ccgcatcgct aagtttgtcg tgacgccatt caccgccaac
    11821 cgtgagaaac tgattaatcg ccgtcagcgg caacgtgtat ttgccgtcga ccgtattgct
    11881 ttcggaagtt atcgggctgc tgttgccagg gtttttgttc tcgactttct caccgtagta
    11941 tttcagttcg ctggtgccgt aatcccaacg cccattatgg ctgacggagt agttctggcg
    12001 ttccaggcgg tttttgtcca gcgagtcgga atcacgatcc tgacggtcga aaccgtatcc
    12061 ggcagtaaaa tcgtgatttt gattcggtgt ccaggcaaat tcgacattgc cgtcgcggct
    12121 ggagaatcct tcaatacgcg gcgtatctcc ggtatcggtg gtcgttgagt tttgcgggtc
    12181 atccttttca cgttttgcca ggctgccgta agctttcatt cccagcacac catcaattaa
    12241 tggtccactg gtaaagaact gaccgttata ggtatcaccg cgatcgcgat gttcctgaat
    12301 ggtggtatcg acggtaacgg tacccgacca tttctgaccg atttttttgg tgatgatatt
    12361 cactacaccg ccgagcgcat cggaaccgta cagcgacgac atcgggccac ggaccacttc
    12421 aatacgttcg atggaatcga ccgggatcca gttcagatcg aaatcattgt ggcggaagac
    12481 ggcattgcgg gagttaacgc gtttaccgtc gacaagaatc agggtgtagc tgctgtccag
    12541 accacgaata ctaacgccct tacggttatc cccttcgttc gtcagttgta cgccaggcac
    12601 ttctttgagg acatccttca gattctgtac cggttttcgc tgcaggtctt cctgggtaat
    12661 gacgctgata ctggcgggtg catctttaag gttttgttca acggaagatg cagtgacaac
    12721 catcgtttcg ccatcatcat cgaccgctaa cacaggccat gcacaagaaa tagcggacaa
    12781 acacagcccg acccgtacga aagggttcaa cctaaacatt ccatatctcc atgaggtaac
    12841 tacgaaaata aaatgggtta tcgctcacat cttcttcacg tccccttgcg tacggcagca
    12901 tcgcggtgga cttatttgta tgcaggtgat catcccagaa agccggaaca gcctctgatt
    12961 tgtcagcctt ttttgattgc ggcgtaacga taatgcaaac gataacaatt atcaatccaa
    13021 atgataaatt ttatatccgc atgatttgtc agggaaatgg aattgaaaaa gccctctcgg
    13081 ttgagagggc ttagcaagga agggaggaaa cttatttctt atcgttctgc gggaacttca
    13141 tttcgctgta acgtacgaag tgtgttcctt taatcagctt gtagccgaac caaataatca
    13201 ggaacagcgg gataccaata tacgttgccg ctacgccgcc ccagtcaatg gtatctttca
    13261 ggaacgcttc gtagttctgg cctaaagtga taatcagaca cagaatgaat gcgaagatcg
    13321 gccccagtgg gaagaaacct gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata
    13381 cgtaaccgcg acggaagcga tagtggctaa tggcaatccc cagccaggcg ataaaaccgg
    13441 tcatcccgga ggtgttcagc agccacaggt ataccgtctg gttgccaaac atggaggtca
    13501 ggaagcacag accggcaatc accgtcgtcg catacagcgc attacgcggc acgccaccac
    13561 gcgacagttt agcgaaaatg cgcggcgctt taccgtcaca cgccagggtg tacagcatac
    13621 gagtagacgc atacataccg gagttacccg ctgacagcac cgccgtcaga ataactgcat
    13681 tcatcaccgc cgccgcagag agcagacccg cgtgctggaa caccagggtg aacggactaa
    13741 cgctgatgtc tttaacatcg ttacgcagca ggctcggatc ggtgtacgga ataatcaggc
    13801 tgataatcag gatcgcgaac acatagaaca acaggattcg ccagaacacc tgacgtaccg
    13861 cgcgtggaat gtttttcgcc ggatcttcgg actcgcctgc agcaataccg atcagctcgg
    13921 ttccctggaa agagaagccg acaatcatag ctacgccgat catcgccgca aaaccaccag
    13981 caaacggcgc ttcgccgatt gtccagttgc tccagcccgc aggctgcgcg cctttgaaga
    14041 taccgataat catcagcacg ccaacgatga taaagacaat aactgtcgtg actttgatca
    14101 gtgagaacca gtattccgct tcaccaaagc cgcgaactga gatgtagttc agcaggaaga
    14161 taacgccgag gaacagcgca ctccagatcc agcccggtgt atccgggaac caccagctca
    14221 tgaccagctg agctgcaacc aggtcaacgg cgatagtcac cgcccagttg taccagtagt
    14281 tccagcccag cgcgaagcca aagccttctt caacatagtt ctgaccgtaa gtggcaaacg
    14341 aaccggaaac cggcatatat gcagccagtt caccgagact ggtcatcagg aagtaaacca
    14401 tcaggccaat caacatatac gagagcaatg ccccgcccgg acctgcctga gaaatcgttg
    14461 cgccagaggc aacaaaaaga cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt
    14521 gacgcgcctt taattcacgg cgtaagcccg gcgcttctgt agttttagtt tcggaaccca
    14581 tacaaaaatg ctatccatct taaaaatgag gcgcgattgt agcagacgat tggcaatcct
    14641 tccggcagaa atacacggtt ataagacacc ttcatgatcg cccagggatt ataagtaaag
    14701 cagccaatct cttttctggc gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag
    14761 caccagcatg ttcttgtaca gcaacttaac gcggtacatt cgcgggatcg caatagtcca
    14821 gaaagcgccg tagcgcgttg gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac
    14881 gcatcaggcg cggcagaggg accgcaactt cacttaatgt gcctgcctgc aattgatctt
    14941 caatcacccg ccgcgacagg caactaattc ccaacccatg acgcaccgca tgtttgattg
    15001 cctcggagtt acctaattcc atcgccatct caaacttcgg taaatgtgac agcaacagat
    15061 aatcgacaat ctcccgcgtg ccggagccgc gttcacgcag gatccacggc gcagcggcca
    15121 gctgttctaa ggtgacaggt ccacgagcca atggcgaagt cggcgcggcg aaaaccacca
    15181 gctcgtcttc cagccacggt tcagaaatga tttcagtgct gtggcacggt ccttcaataa
    15241 agccaatatc aacgcggaaa tccagcaccg cttgcatcac gtcctggctg ttcccaacgc
    15301 taagttcaat cggcaactgc ggataatcat ggcgataacg ggcgataact gcaggcagaa
    15361 tgtagttacc gatggtacta ctggcataga tacgaatcgc accgttgtct tcgcgaaaca
    15421 gttgttcgat ttcgatcgcc tgttcaagca gtgccaacgc acgcggatag agcagtcgcc
    15481 cgtgttcatt aactaccagt cttttcccca cgcgatcaaa cagttgcacg ccaagctgcc
    15541 cttccaggtc ggtcaaggct gcgctcactg ctgattgcga caacgccagc atcaccgacg
    15601 cctgggtggt tgatccactt ttcaatactt ctgcaaaaac ttccaactgc cggagggtga
    15661 tgtgcatagt cgcttaccac ttataaagat taattataaa tatataatca attttatttt
    15721 taaaccagtt agtcgtaacc ttatgcccgg ttaaaggaga gggttatgac gaatatcacc
    15781 ttacagaaac aacatcgtac actgtggcat tttattccgg ggttagccct gagtgcagtt
    15841 atcaccgggg tcgccctgtg gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt
    15901 gccctcaccc tcgcaatctt gttggggatg gttttaggca acaccatcta tccgcacatc
    15961 tggaaaagct gtgacggtgg tgtgctgttt gccaagcaat atttattacg tctgggtatc
    16021 attctttatg gcttccgtct gacgttctcg caaattgccg atgtcggtat cagtgggatc
    16081 atcattgacg tgttgacgct gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa
    16141 gtgtttggtc tggataagca caccagctgg ttgatcggtg ccggtagcag tatctgtggt
    16201 gctgccgcgg tactggcgac tgagccggta gtgaaagcgg aagccagtaa agtaaccgtg
    16261 gctgttgcaa ccgttgttat cttcgggacc gtcgcgattt tcctctaccc ggcgatatat
    16321 ccgctgatgt ctcaatggtt tagtccggaa actttcggta tctacattgg ttctactgtg
    16381 cacgaagtgg cgcaggtggt ggcggcaggt catgccatca gcccggatgc agaaaacgca
    16441 gcagttattt ccaaaatgct gcgcgtgatg atgctggctc cgttcctcat cctgctggcg
    16501 gcgcgtgtta aacagctgtc tggggcgaac agcggcgaga aaagcaaaat cactattccg
    16561 tggtttgcca tcttgttcat cgtagttgcc atctttaact cgttccacct gttaccgcag
    16621 agcgtggtga acatgctggt aacgctggat accttcctgc tggcaatggc gatggcggcg
    16681 ctgggtctga ccactcacgt cagcgcactg aaaaaagccg gggcgaaacc tctgctgatg
    16741 gcactggtac tgtttgcctg gctgattgtt ggtggtggtg cgattaacta tgtgattcaa
    16801 agcgtcatcg cataaaccac tacatcttgc tcctgttaac ccgctatcat taccgttttc
    16861 ctccagcggg tttaacagga gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc
    16921 tggcggtctg gcaaatgccg caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt
    16981 gttcaccaaa aaccaacgtc agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga
    17041 attcaaagcc gcctgtgaaa aatatcacta cacatcggcg caaattcttc cccacgacag
    17101 ttatctgatt aacctcggac atccggtcgc tgaagctctg gaaaaatcgc gcgatgcgtt
    17161 tatagatgaa atgcagcgtt gcgaacagct ggggctttct ttgctcaact tccaccctgg
    17221 cagccatctg atgcagattt cagaagagga ttgccttgcg cgtattgccg aatccatcaa
    17281 cattgcgctg gataaaactc aaggtgtgac agcggtgatt gaaaacaccg ccggtcaggg
    17341 cagtaactta gggtttaaat tcgaacatct cgcggcgatt atcgacggcg tggaagataa
    17401 atcccgcgtc ggcgtctgca ttgatacctg ccatgctttt gctgccgggt atgatttgcg
    17461 tactccagcc gaatgcgaga aaacgttcgc ggattttgcc cgtattgtcg gctttaagta
    17521 tctgcgcggg atgcacctta acgatgcgaa aagcaccttt ggcagccgcg ttgaccgcca
    17581 tcatagcctc ggtgaaggca atatcggtca tgatgcgttc cgctggatca tgcaggacga
    17641 ccgtttcgac ggcattccgc tgatccttga aaccatcaac ccggatatct gggcagaaga
    17701 gatcgcctgg ctgaaagcgc aacaaactga aaaagcggta gcctgaagat gaacaaccgg
    17761 gagaaggaga tccttgcaat tttacggcgt aacccgctga ttcagcagaa cgaaattgct
    17821 gatatgctgc aaatcagccg ttcgcgcgtt gcggcgcata ttatggattt aatgcgcaaa
    17881 ggtcgaatta aaggcaaagg ttacattctc accgagcagg aatactgcgt agtggtgggg
    17941 acaatcaata tggatattcg cgggatggcg gatatccgtt acccgcaagc ggcttctcat
    18001 cccggtacca ttaattgctc ggcaggcggc gttggacgca atatcgccca caatctggcg
    18061 ctgttaggcc gcgacgtcca tttgctttca gtgattggcg atgactttta tggcgaaatg
    18121 ctcctggaag aaacgcgccg cgccggcgtg aatgtctccg gctgcgttcg tttacatggt
    18181 caaagcacat cgacgtatct ggcaattgcc aatcgagacg atcaaaccgt gctggcgatt
    18241 aacgataccc atctgctgga tcagttgaca ccgcaactac tgaacgggtc gcgcgattta
    18301 cttcgtcatg cgggcgtggt actggctgat tgcaacctga cagccgaggc gctggagtgg
    18361 gtctttaccc tcgctgatga gatcccggtg tttgtcgata ccgtttcaga attcaaagcg
    18421 ggcaaaatca aacactggct ggcgcatatt cacaccctga aacccacttt accggagctg
    18481 gaaattttat ggggacaggc gatcaccagc gatgctgacc gtaatgccgc agtgaatgcg
    18541 ttgcatcagc aaggcgttca gcaactgttt gtttatttgc ccgatgagtc agtttattgc
    18601 agcgaaaagg atggagagca atttttgctg accgcgccag cgcatacgac agtagacagt
    18661 tttggtgctg acgatggttt tatggcgggc ctggtatata gctttctgga gggaaacaat
    18721 ttccgtgaca gcgcccgttt tgcgatggcc tgcgcggcaa tttcacgcgc cagcggcagc
    18781 ttaaacaacc ctaccctgtc tgccgataac gcgctgtcat tagtgccaat ggtgtaacaa
    18841 tgttgccgga tgcgacgctt aacgcgtctt atccggccta caaatcaggc aatttcagca
    18901 agtaaatgta ggcctgataa gcttgcgcat caggcaatga cactcaagct aaaccaataa
    18961 agaacccggc aatggtcgca ctcatcaagt tggaaagcgt cgccgccgcc agcgcgcgta
    19021 aaccaagctg ggcgatttcc ggcgcacggt gtggcgcaac cgcagaaaat gcgccaacca
    19081 ccaccccgat agaaccaaag ttagcgaaac cgcacaacgc gaaagaaata atcgccacgg
    19141 ttttagcatc cagagtgcca gctgtttgca gataaggtga gaaattgagg taagcgacaa
    19201 attcatttat tgccagcttc tgtccaatca aactcccggc aagattcgca tcactccagt
    19261 caacccccat cacccatgcc agtggagcca acaggtaacc taaaatggac tccagcgagg
    19321 catgttcaaa accaaaccag ccgccaacgc cgccgataat accgttaatc aacgcaatta
    19381 ttgcaacaaa tgccatcacc actgtcgcca cacctgcggc gattttcagc ccggtcattg
    19441 cccctgtagc ggcggcttca ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat
    19501 taaaggaaac ctgcgaagat tccgtagccg ggcttaacag gcgggcaaac aagatcccgc
    19561 cggggatcgc cattaacgat gccgctagca gatattccac aggcacgccc agtgcggcgt
    19621 aaccaatcat tgtcgaacca gcaatcgagg ccatgccact acaaatagcc gtaaataatt
    19681 cattgcgatt cagacgatcg ataaagggtt tgacgattgc cggaatttcg ttttgcccga
    19741 ggaaaatggt agtgaccgcg acgaatgact caatcttgct gatattcaat gctttctgga
    19801 atataccgcc gagaatgcga attaagatcc ccatcacacc gatgtagtag agaatactca
    19861 ccagtgctgt aacgaagata attgccggta ataccctgaa accaaagata aatccagcgc
    19921 catcaaataa ggtgtccatt ttcggtccga ccagagaacc aaagataaat gcgctacccg
    19981 cgtcgctgta cgccatcact ttatgcacgc caaaagcgac tttttcagcg acccaacgcc
    20041 ctggcggtaa ccaaagcata atgccgccaa tcacgacctg taacactaac gccgcgccaa
    20101 cggtacgcag gctgatcttc ttcttgttta ctgacagcaa aaacgcaatt gccagcaata
    20161 ccaccattcc cagaacactt ctcatgacat ccatagttat tatctcttca tgtcattttg
    20221 ggcgacggct ttctgcccat cagaaatagc cgggcctgca tcttgcgata ctgacccggc
    20281 ttgttatcaa cacttaatgg gttttgatgt agccgcgcac gcactcttcg actaatcccc
    20341 agaaccagtc ggtatcaata gtgatgccga ctttggtatt agcgggcttg ccaagaacgc
    20401 ccagctcgtc gcagacagta cgcccatagc aagggccact gttgacgtcc acttcgacgt
    20461 acatctcctg ggttttaatg ccatcagggt taatcagata accgatgcag gtggcgtcat
    20521 gcaccgggcc gccagccagg ccgtagtttt cgaactgcgt ttttagagtg aaattcatga
    20581 tgtcgctgaa cagctctccg gcggggccgc ctaccctttc catccgggca atcacgtccg
    20641 gggtgcaaac agtctggttg gtgagatcga ggcccatcat caccaacgga acgccggagg
    20701 tgaacaccac gcgtgcggct tccgggtcgg caaagatgtt gaactcggca gatggggtga
    20761 agttgcctgt accgtaagcg ccgcccatta gcacaatttc acggattttc ggcaggatcg
    20821 cgggttgcat acgcattgcc accgcgatat ttgaaagcgg accaaccggc accagagtga
    20881 tatcaccatc gcttgccatc agggtatcaa tgatatattt caccgcatga gtgctttctg
    20941 cctggcgggt cagcggcccg aataccgggc catcaagtcc ggtttcaccg tgaatattat
    21001 cggcaacgat ttgttgacgc ataatgggct gtggcatccc cgcataaacc ggaacattaa
    21061 tctccagttt ctggcaaaca ttcaggccat taattaatgt tttatcaagc gtctgattac
    21121 ccgccacaat ggtgatgcct aataaatcaa tcaccggatg tttcgcggcc atcattatgg
    21181 caatagcatc atcatgaccc ggatcacaat ccagaataat ttttctcttt tccattgttt
    21241 atttcctctg tttccagttg cgttattttt tctacagcaa agaaaattcg accgggccga
    21301 tgattgaatc ttaacaacag cgtaagtatg ctaaatatga gaaatctcat agcggataaa
    21361 catcgtgaaa gaaatccaca ataatgatct taagcagcaa ttgatgagtg aatccgcgtt
    21421 taaggattgc ttttcaacgg atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg
    21481 tgactacata gtgcaggaag ggcaacagcc gtcctggctg ttttatctga cgcgaggccg
    21541 cgccaggctt tacgccacgc ttgctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc
    21601 tccctgtttt attggcgaga ttgagttaat cgacaaagac catgaaccgc gtgcggtgca
    21661 ggctattgaa gagtgttggt gccttgcgct ccctatgaaa cattaccgtc cactgttatt
    21721 aaacgacacc ctatttttac gaaaactttg cgtcacctta agtcataaaa attatcgtaa
    21781 tattgtttct ttaactcaga atcaatcatt tccgttagtg aatcgcctgg cagcttttat
    21841 attactctca caagaaggtg atctttatca cgaaaagcat acgcaagcgg cagagtattt
    21901 aggcgtttct tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg acggtttatt
    21961 aattaaaagc aagaaaggat atctaattaa aaacagaaag cagttgtcag gactggcgct
    22021 ggagatggac ccggagaata aattctccgg gatgatgcag taaaaaataa tctgcaatag
    22081 cgcgattgcc ggatgcaacg cttaataagg cgtacatccg gcaatgaaac tcaagctaaa
    22141 ccaataaaga acccggcaat ggtcgcgctc atcaggttgg aaagcgttgc tgctgccagc
    22201 gcccgtaaac caagctgggc gatttccggc gcgcgttttg gcgaaatagc cgaaaatgcg
    22261 ccaacgacaa caccgataga accaaagttc gcaaaaccac aaagcgcaaa ggagataatc
    22321 gccacggttt tcacttccag cgtgccgccc gtttgcaggt atggggagaa actcaggtaa
    22381 gcgacaaatt cgttaatcgc cagtttctgc ccaatcaggc tacccgcaag gttggcatca
    22441 ctccagtcca cacccatgat ccacgccagc ggtgccagca catagccaaa aatactttcc
    22501 agagaggcat tggcgaaacc aaaccagccg ccaattccgc cgataatgcc gttgatcagc
    22561 gcaataattg cgacaaacgc cattaccacc gtcgctacac cagcggcgat tttcagcccg
    22621 gtcatcgcac cgctcgccgc tgcttcgata aagctttttg gcggcgtttc gctgaacgac
    22681 agattttcaa atgtgacctg cgaaggctcg gttgccgggc taagaatacg tgcaaacaaa
    22741 ataccgccag ggatcgccat cagcgatgcc gctaacaggt agtcaattgg tacgcccatt
    22801 ccggcataac caatcatcat cgaaccagca atggacgcca tcccgctaca aatagccgta
    22861 aacaactcgt tgcgattcat gcgatcgata aacggtttaa cgatcgccgg gatctcattt
    22921 tgcccgagga aaatagtagt aaccgcaaca aaagattcga ttttgctgat gttgagggct
    22981 ttctggaaaa tgctgccaag gatgcgaatc agcagcccca tcacgccaat gtagtacagc
    23041 agactgatga gcgcagtaac gaagataatc gccggaagta cgcgaaaggc gaagataaag
    23101 cccgcgccat caaacaggac atccattttc ggcccgacca atgaaccaaa aataaaggcg
    23161 ctaccggcat cactgtaaga catcacttta tgaacgccta atgccgcctg ttctactgcc
    23221 cattttcccg gtgggaagta gagcatgatg ccaccaatag cgatttgcag cagcagtgcg
    23281 gctccaaccg tgcgcaaact gatgcccttt ttattcactg acaacagaaa tgctattgcc
    23341 agtaacacca ccatccccac aacacttctc attatatcca taatgatttt cccttcatgc
    23401 cagtaaaccc ggcgtcagcg ccaggttttg gtatgcttga tgagtacggg cgacggcttt
    23461 ctgcccgtca gaaattaacc cgcgagacgc tggtattctt tggcaatttc gctcgccaga
    23521 atggcgttgt tgaacaccag ttggatgttg gatttcaggc tgtcaccgcc agtcagttca
    23581 gcaacacgag ccagcaggaa cggtgtgctt tctttaccaa taacgccctg ctcttcggct
    23641 tcggcgacgg cttgatctat ggctgcattg atagattcct ccggcatcgc aaactgttcc
    23701 gggatcggat tcgccaccac caggccaccg ttcagaccgc tttgccattt caccgccatt
    23761 gcacgagcga tttccgtggc actatcaagg cgaatactga cttcaaacgg gctggtgcgg
    23821 cagaaaaacg caggcagcgc tttggtctga tagccaatta acggcacacc gaaggtttct
    23881 aaatactcag tggttaatcc taaatcgaga atagatttcg ccccggcaca aacaacggtg
    23941 acattagtat ttgccagttc ttgcaaatcg gcagaaatat cgaaggtatg ttccgccccg
    24001 cgatgcacac caccaattcc ccctgtggca aatactttaa ttccggcaag cgccgcaata
    24061 atcatcgttg aagcaacggt agttgcgcca ttttttccgg cggcaacaac aaaaggtaaa
    24121 tcgcgacgac taactttggt cacgttatgc ccttcactac ccagcaattc aatttcttct
    24181 ttgcttaaac ccactttcat cacgccgcca ataatggcga tagtggcagg tacagcgccc
    24241 tgtttacgaa tagtttcttc aacttcaatt gcggtctggg cattttgtgg gaacggcatc
    24301 ccgtgagaaa taatggtcga ttccagcgcc acaaccggtt ttttgttttt taaagcgtcc
    24361 tgcacttccg gggaaatttg taataattca ggggaaattt ttaattcaga cattctgcgt
    24421 tctccactaa cgatataacg ttggcaatcg ataaatcggg gttattggtg tattcacagg
    24481 agagcgccat tgacgaacat ccctgtgcga aacgaacaga ttcggcaaac ggcattccgt
    24541 ctacccaaca cgaagcaagt cccgccatca tggcatcgcc cgctccggta acattaatga
    24601 cattggtttt gatcggcgca gaccaaccac tttcaccgct gatatcactg taataaacgc
    24661 cgtcgccgcc catgctcatt accagtcggt tcaggccatg ttgatggaac caggcagcaa
    24721 cttttgccac atcttcacgc cctgacaaag caatcccact caaggtttcc gcttcaaggc
    24781 ggtttggctt cagggtgtgg atctgattta gacggtcacg cactttgaca catttccatg
    24841 ccgaaaccgg atcgacaaat acaggtacat tggcggcatt atccagaatc catgccagtg
    24901 cctcttcact gatattacag tccgcgacaa tgacctttgc cctttgaata aattcacggt
    24961 gctgtgcgag atattcagct gtaatagcgt tgctaatatt catgtcattt atagcaacca
    25021 gcatttcacc ggtattatcg agtaatgata aatagctcga cgtgttttct cccggcacaa
    25081 tcaggcattt atcgacataa acaccagatt gattggtttg cgttagcaac gattgaccat
    25141 aaaaatcact gcctactgcg ctcagtagcc aggatttatt ccccagcaac gccaggtttt
    25201 gtgcaatatt gcgcccgact ccaccaggcg taaattttat tttacctgga tttgaatccg
    25261 cataatttaa tgattcatgt gaatatccgg cgacatcaat attcgccgaa cctataatta
    25321 cgacataatc cttttcgcgc atagcacgtc ctctggtaaa aaattaccaa ccctgtcaat
    25381 ccacaagaat attgatactg caaagacggt ttaaacatgt gttcatattt aaacatgtgc
    25441 tcatagtatg catgttttac gtaaagtaaa ttaccgccag agaggcaaaa tgggcagatt
    25501 tgagcaggct cacaaaacgc gattaaatgc ctgaattata aaaacgggga atttgtgacg
    25561 ggatgcacag aacaggaaca ataaaaaagg gcagaaatta atctgccctt gaggaaataa
    25621 ccttagtgtt tgatgaaggt ttcaggtttc tactaaaaga taaaaccatt taaattcata
    25681 aaattacatt cacaaaccgg tcattctctt tgcgcgcatc aaatgttaca gggcaggaaa
    25741 tttctgccct gtaacacacc ttttattacg ctgctttcgc tactgcgtcc acttccggac
    25801 gtttcaggaa ggcataggcc agacccgcca ccagcgtacc ggcaataatg gctaccaggt
    25861 aacccagtac cggcgtaata gcgccaggga tcagcagaac aaacagacca ccgtgcggtg
    25921 ccatcagttt cgcaccaatc gccattgaga ttgcgccagt cagcgcccca cccacgatac
    25981 agcacggcag cacacgcatc ggatcacgag cagcaaacgg aattgcacct tccgaaatga
    26041 agcacagtcc caataccaga gcggctttgc caccttcctg ctgcgctttg tcgaatttgc
    26101 gacgcgccac cattgttgcc aggcccattg ccagcggtgg caccatacct gccgccataa
    26161 tcgccgccat cgggccatag gtttgagtac tcagcagacc cacaccgaat gcgtacgctg
    26221 ctttgtttac cggaccgccc atgtcagtac acatcatgcc accgaggatc gcccccagca
    26281 gaaccgcatt cgcagtcccc atggtctgca gccagtgagt caacccttcg agaatgccag
    26341 caaccggttt accgatcagg tagatcatcg ccagaccgac caccagactg gaaattagcg
    26401 gaatgatcag aatcggtttc agcgcctcca tactctgtgg cagtttcaat tgcgtactga
    26461 ttaacttcgc aatgtaacca gccaggaagc ccgcaataat accgccaatg aagccagaac
    26521 cagtgctgac cgccagcata ccgccaatca gacccggcgt gagacccgga cgatcggcaa
    26581 tggaaaaggc aatataacct gccagtaccg gcaccatcaa cgcaaaggct gaaccaccac
    26641 caatctgcat cagcgccgca gccaacgtac ccggctcttt aaacgcttcg ataccaaaag
    26701 caaaagaaag cgcgatacac agaccacctg caacgaccat cggcagcatg taagagacgc
    26761 cagtcagcaa gtgacggtaa gcgcctgcac tctctttctt accttcagtg gtcgctgttt
    26821 gagttttgcc cgccggttca tacggcgttg cttcagcaac cgcattatcc agttcctgcg
    26881 cggttttctt cagcgccaga ccggtagagg tacgatacat cggtttacca gcaaatttcg
    26941 ccagatccac ttcgatatct gccgccacaa tcaccagatc cgctgctgcg acttcttcgg
    27001 gagtgattgc attacccgcg ccaacagaac cacgggtttc aactttcacc caccagccac
    27061 gttttttcgc ttcggtttca atggcttcag ccgccataaa ggtgtgtgct acgccagtcg
    27121 ggcaagcagt caccgcaact acgcgtttcg gaccgctggc ggcaaccggt gctgtcgcag
    27181 cgaccggcgc agtgtaaggt ttcgcatggc ctttggcttc actcaggaac agctcagggt
    27241 gcgcaactgc ccgggaaata tcgcccagcc agacattttt accgttcagc gcgctgtcgt
    27301 tcgggatgga atcaccgaga acaatcgcca tttcagcgtc gttcggattg tcgatgattt
    27361 ccagttttgc ttttcgcgcc gccgcgccca gcagggtctt cgccatatag gcgcgtgcct
    27421 gaccgagatt agcgtcaata atcagcagcg ttttcattat gcctctcctg ctgtcagtta
    27481 aaaggttgta agtcgacgcg cgccatcatt gcggccaact gcggacgatc ggtaataccc
    27541 acattgcttt gacttaccgc cagggctgca acagctgtcg ccagacgcag tgtgtgttca
    27601 ctggattcac gcatcagcaa gccataaatc aggccgccaa ccatagaatc ccctgcgcca
    27661 acggtgctta cgacatcgac tgacggtggt ttggcgatcc attcgccgga ggcattaacc
    27721 caaagcgcgc cttcggcacc cagtgaaata acgacatgcg cgataccttg ttcacgtagc
    27781 gcatgtgcag cttcaatcac atctttcatt tcaggcagtt tacggcctgc ccagatttcc
    27841 agctcgcggc ggttaggttt caccagccac ggtgccgctt tcaaacctgc tactaacgct
    27901 tcgcggctac tatcaaagat aatgcaagga cactgactac gcaggcgagt catccagtcg
    27961 gtgaacgctt ccgggctgac gcctgacggt aagcttccgc tgacacagac catatcgaac
    28021 tgaccgagcc agctcagaga atcagtcaca aagcgttccc agtcggcggg ggtgacttca
    28081 aaacccgaga agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt aacgttaatt
    28141 cgggtgcgcc cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct gaacagttgc
    28201 tgaaaaccat cctgattgtc tttaccgagg aagccgccaa cggtgacatc aattcccagg
    28261 tcttttaata ctttggccac gttgatgcct ttacccgccg catgcagacc ggtggttttc
    28321 accaggttca cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc ataagccgga
    28381 ttaagggtga tagtagcaac acgtctgctc attatgcgcc ctccccaaga ccagcagcga
    28441 tagcgtcgcc aatggctttc agcgcctgtt cagcatctgc accctgggcg gtaaagcgta
    28501 ggcgatgacc tttcttaacg ccaagtgcca caactttcat cagactacgt ccgtttgccg
    28561 gtttgccggt gccatcaagg tttgtcacgg taatatcact gttaaattgt ttaatggtat
    28621 tgaccagcat ggtacctgga cgagcatgca ggccgtgttc attgcgcacc acaaactccg
    28681 cgcttaacac gtcgtcggtc ggcgcatcat cgctggtcag caacgccagc aacgttgccg
    28741 catccgcttt cagcaagcgg tcagctttat tgtcgagtaa caaatcagcg agacgcttaa
    28801 gaaccgcaat gggctgatcg tcattcatcg ccacactcac cagcatggct gccgtttcgc
    28861 cgtccacatc aaaagcattt gccgcacggc ttaccgcaat cgcgctacgc agattgcctt
    28921 cggcgctatc gctcagccag ataccctgtc cgaggttcag cggttgttca ttgatggctt
    28981 tggtgacgaa agtggcgtca actgcccccg cctctttcag acgagcagcg ttcagcgcct
    29041 gaagagtcag cagatcgctg gcgacgatat ccagcgtcag catttcgttg tcgagcttca
    29101 gctgctcact ctgcttttcg cccatcagca atgcgcgaag ttcttctgct gttgttgctg
    29161 acttcagttg ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg cgtagcaggc
    29221 ccaaatgttc atccgagctg gcagcaatac cgatcgccac gtacgctacc tggccgtcac
    29281 cccaggtgac gccttccggg aactgaaata cctgaacgcc ggttttcagc acctgatcgc
    29341 gggtgtcggt agtgccgtgt ggaatagcaa taccattgcc gaggaacgta gaggtttgct
    29401 gttcgcgcgc cagcatgcca ttgacgtagc cttcagctac attaccggcc tgcaccagcg
    29461 ccgcagcgac ctggcgaatc gcctcttctt tgtctccggc cttttcgccc ggatggatgt
    29521 cctgtacgga taactggaac atagttctcc tctcttgctg aattgaaacg attcagcttc
    29581 tatgagaaaa aaagcgccaa cctggcttag ggttaaagac aagatcgcgc tgaaacgttt
    29641 caagaaagca taatacttct gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa
    29701 gtttgatgtg ctgcacaatt ttttgtcatt tttcctaatt gctgatggga aaatccgtta
    29761 tcagcaattt catttcagca gcgtcagcag ccaatcagca acagtgaaat ggcattttga
    29821 ttattcgtgg cgcttttgat ttcgctgtct gtttattttc tgacaagcag cgtaaactcc
    29881 gcgtcttcct cttccagtga tcgaccagca tgcataactc ccccgcagtc tccagcgcga
    29941 aatcgtttga cctgacctcg acggcgtttt taatcgttgc ttttctcacc ggtattgcgg
    30001 gcgctctgca aaccccgaca ctcagtattt ttcttactga tgaagtacat gcccgcccgg
    30061 cgatggtggg attcttcttt accggcagcg ctgtcattgg gattctggta agtcagtttc
    30121 tcgccgggcg ctctgataag cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt
    30181 taggcgtgct ggcctgcact ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg
    30241 ttggcgtctt tcttagcagc tttggctcga ctgctaaccc gcaaatgttt gccctcgccc
    30301 gtgaacatgc tgacaaaacc ggacgtgaag cggtgatgtt cagctctttt ttacgcgctc
    30361 aggtttcact ggcatgggtc attggcccgc cgctggctta tgccttagcg atgggtttca
    30421 gctttacggt aatgtatctg agcgcagcgg tagcgtttat tgtttgcggc gtgatggtgt
    30481 ggctgttttt accgtcgatg caaaaagaac ttccactggc gaccggcacg gttgaagcgc
    30541 cgcgccgtaa ccgtcgcgat acgctgctgc tgtttgtcat ttgtacattg atgtggggct
    30601 cgaacagcct gtacatcatc aacatgccgc tatttattat caacgaactg catcttcccg
    30661 agaaactggc cggtgtgatg atggggaccg ccgccgggct ggaaatcccg accatgttga
    30721 ttgccggata tttcgccaaa cgtctgggta agcgtttctt aatgcgcgtt gctgccgtgg
    30781 gtggcgtctg tttttacgca ggaatgctga tggcgcattc acctgtcatt ctgttgggct
    30841 tgcagctgct aaatgctatt tttattggca ttctgggtgg catcgggatg ctctattttc
    30901 aggatttgat gcccggtcag gcaggttcag ccaccacgct ctataccaac acttcgcgcg
    30961 tgggctggat catcgcagga tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg
    31021 ctgtgttctg gtttgcgatg gtgatgatta tcgccactct gttttgctta ctacggatta
    31081 aagatgttta aggcgcggtc aggatttcca gatcgataag ccaggtcatc gcctgctgac
    31141 gcgagttacc gcacatttca gcgctggctt gtaagcctgc acagactttc ggtcgcagtg
    31201 gcgaggtgaa aattttacag cgttgctgtt catcaagctg aatgcagggc gtattggcgg
    31261 gcttgccatc tggcataccg ggaatagggc tggaaattga aggggcagtg caacacgccc
    31321 cgcaacccgg acggcattcc atacgttgtt ctcttaaggt taaccaccgg attgatgagc
    31381 gcgcacagta ccaccttttt tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc
    31441 cgtcgcgagt actttgtcgc gatatttttg acattttcga ctacaggaat ttttcgatgc
    31501 caagagcgaa cgaaattaaa aaaggtatgg tactgaatta caacggcaaa ctgctgctgg
    31561 ttaaggatat tgatattcag tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc
    31621 gtttttctga tgtccgtacc gggctgaaag tagaagagcg tttcaaagga gatgatatcg
    31681 ttgacaccgt gacgctgacc cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat
    31741 atgtctttat ggataaagaa gactataccc cgtatacctt caccaaagat cagattgaag
    31801 aagagttgct gtttatgcca gagggcggca tgccggacat gcaggtgctg acctgggacg
    31861 gtcaactgct ggcgcttgag ctgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac
    31921 ctggcatcaa aggggcatct gccagcgccc gtaacaaacc ggcgacattg agcactggtc
    31981 tggtgattca ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc catatcgaag
    32041 aacgccgtta tatgggtcgt gctgactaac ttcagccgca tgcagaaaag ggatagctca
    32101 ggctgtccct tttttaattt attataccaa tcttctattt tgcgcttcct gaacacctta
    32161 cgtcctcaat tttcactcgt tgattgatcg ccctcacact tcatcgcatt aacaatccag
    32221 accaatttca attgctgtca tataacttta cactgtcatt gttaattaat cgttactaag
    32281 acgtgactcc tatgaataca atcgcctccg ttacgctccc gcatcatgta cacgctccac
    32341 gctacgatcg ccagcagttg caatcacgta tcgttcattt tggctttgga gcctttcacc
    32401 gcgcacatca ggcgttactg accgatcgtg tgctgaatgc ccagggcggt gactggggga
    32461 tctgtgaaat cagcttgttc agcggtgatc aactgatgag ccagctccgc gcacagaacc
    32521 atttatatac cgtgctggag aaaggggcgg acggcaatca ggcgataatt gtcggtgccg
    32581 ttcacgaatg ccttaatgca aaactggatt ccttagcggc aattattgag aaattttgcg
    32641 agccacaggt ggcaattgtt tccctgacga ttaccgaaaa aggctattgt attgacccgg
    32701 ccaccggtgc actcgacacc agcaatccgc ggattattca cgatctacaa aaccctgaag
    32761 aacctcactc cgcgccgggt attctcgtcg aagcactgaa acgacgccgt gaacgcggcc
    32821 ttgcaccgtt taccgtactc tcctgcgaca atattcccga caatggccat gtggtgaaaa
    32881 acgcggtgct ggggatggcg gaaaaacgtt caccagaact cgccgggtgg ataaaagaac
    32941 acgtcagttt tccgggaacc atggtcgacc gtattgttcc ggcagcaacc gacgaatcac
    33001 tggcggaaat cagccagcat ctgggggtga atgatccctg cgcgattagc tgcgaaccgt
    33061 ttatccagtg ggtggtggaa gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag
    33121 gtgtacaaat ggtgaatgat gtcctgccat gggaagagat gaaactgcgt atgcttaatg
    33181 gcagccactc ttttctcgct tatctgggtt acctctcagg attcgcccat atcagtgatt
    33241 gcatgcagga tcgcgcattt cgccatgcct ccagaacatt aatgctggat gagcaagcgc
    33301 caacattgcg aattaaagat gtcgatttaa cacaatatgc ggataagtta attgcacgtt
    33361 ttgctaatcc ggcgctgaaa cataagacct ggcaaatcgc gatggatggc agccagaaat
    33421 taccgcaacg tatgctggca ggtattcgca tacatctggg gcgcgaaacg gactggtcgt
    33481 tgctggcatt aggcgttgca ggctggatgc gttacgtcag cggcgttgat gatgccggaa
    33541 atgccattga tgttcgcgat ccgcttagcg ataaaattcg cgaacttgtt gcgggcagta
    33601 gcagtgaaca acgcgtaacc gccctgcttt ccctgcgtga agttttcggt gatgatctgc
    33661 cagataaccc gcattttgtg caggctatcg aacaagcctg gcaacaaatc gcacaattcg
    33721 gcgcgcatca ggcgctatta aacaccctca aaatttaacg atttctgcgg ttaaagcgga
    33781 tgaagctcgc cttcgtccgc tccccccttc tcttttctgc cttttttagc caggattaac
    33841 gctcagttaa cttaccagag tattgcggag ccgttgtgac caggaccaac ctcatcaccg
    33901 gttttctcgg cagcgggaaa accacgtcga ttcttcatct gttagcccat aaagatccca
    33961 acgaaaaatg ggcggtactg gttaatgaat ttggggaagt cggaattgat ggtgctttgc
    34021 tcgccgatag cggcgcattg ttgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg
    34081 gtttacccat gcaggtagga ttgaatacct tactgcgtca gggaaaacca gaccgcttgt
    34141 tgatagagcc gaccggactg ggtcatccga aacagatcct agatctctta accgcgcccg
    34201 tgtatgaacc gtggatagat ctgcgcgcca cgttgtgcat tctcgatcca cgcctgctgc
    34261 tggacgaaaa aagcgccagc aatgaaaact tccgtgacca gctggctgcc gcagacatta
    34321 ttgtcgccaa taaatccgac cgtgcgacgc ccgaaagtga gcaagcgcta cagcgctggt
    34381 ggcagcaaaa tggtggcgat cggcaattaa ttcacagcga gcatgggaaa gttgacggtc
    34441 atcttctgga tttgccgcgt cgcaatttag ccgagttgcc cgccagcgcc gcacattctc
    34501 atcatcatag cgtgaaaaaa gggttagcag cgttaagcct gccagagcat caacgctggc
    34561 gtcgcagtct gaacagcgga caagggcatc aggcctgtgg ctggatattc gacgctgata
    34621 cggtattcga caccattggc attctggaat gggcgcgact tgcaccggtg gaacgcgtca
    34681 aaggcgtgat gcgtattccc gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc
    34741 acattgaaac gcaaaacgtt gcgccaccgg acagtcgtat tgagctgatt tccagcagcg
    34801 aagctgactg gaatgccttg cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt
    34861 aaggttgcct gcgtttttca gtaagataat tagagaaaat atgattaaaa atttgccgca
    34921 aatagtgttg ttgaatattg tcggcctcgc gctgtttctt tcctggtata ttcccgttaa
    34981 tcatggattc tggttgccga ttgatgcgga tattttttat ttctttaatc agaaactggt
    35041 cgaaagtaag gcctttttgt ggctggttgc attgaccaac aatcgcgctt tcgacggttg
    35101 ttctctgctg gcgatgggaa tgttgatgct gagtttctgg ctgaaagaaa acgcccctgg
    35161 cagacgacgt atcgtgatta ttggtctggt catgctatta actgcagtgg tattaaacca
    35221 gctgggtcag gcattaattc ctgtaaaacg ggccagccca acattgactt ttaccaatat
    35281 taaccgcgtc agcgaactgc tctctgtccc cacgaaagat gcctcacgag atagctttcc
    35341 cggcgatcac ggtatgatgc tgcttatttt ttcggcattc atgtggcgtt atttcggcaa
    35401 agttgcaggc cttatcgccc ttattatttt tgtggttttt gcatttccca gagtaatgat
    35461 tggcgcacac tggtttactg acatcattgt aggttcgatg accgtgatat tgatcggttt
    35521 gccctgggtg ttgctgacgc cattaagtga tcgattaatc accttttttg acaaatcact
    35581 accgggaaaa aacaaacatt tccaaaacaa ataactcaca gtaattaaca tcatcagggt
    35641 tatttttata gtgaggataa tcctgatgat gcgcaccgtg ctttcatcca tcgcgcgcaa
    35701 aaatcattct ctaagtaaat gaatggattg catgcgtttc actcaatcgt actttaattg
    35761 accaatcccg cttattaact ttctgtatca ctttttctta taaaaaatca tgtaaaaccg
    35821 ctcgccaaga ccgcaccaat cgggtaatct cgaactcgtt ttgcctcggc ggtagattat
    35881 cctcacagca tataattttg tgcgttagtc cacagatttg gccttaagga attgtttcaa
    35941 catgcccagg taattagtct cgtgtcgctt ggcatttttt tataacgata tttgtcgtta
    36001 aggacttcaa gggaaaacaa acaacatggt caaatctcaa ccgattttga gatatatctt
    36061 gcgcgggatc cccgcgattg cagtagcggt tctgctttct gcatgtagtg caaataacac
    36121 cgcaaagaat atgcatcctg agacacgtgc agtgggtagt gaaacatcat cactgcaagc
    36181 ttctcaggat gaatttgaaa acctggttcg taatgtcgac gtaaaatcgc gaattatgga
    36241 tcagtatgct gactggaaag gcgtacgcta tcgtctgggc ggcagcacta aaaaaggtat
    36301 cgattgttct ggtttcgtac agcgtacatt ccgtgagcaa tttggcttag aacttccgcg
    36361 ttcgacttac gaacagcagg aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg
    36421 tgatttagtt ctgttccgtg ccggttcaac gggacgccat gtcggtattt atatcggcaa
    36481 caatcagttt gtccatgctt ccaccagcag tggtgttatt atttccagca tgaatgaacc
    36541 gtactggaag aagcgttaca acgaagcacg ccgggttctc agccgcagct aataaaccgt
    36601 ttggatgcaa tcccttggct atcctgacga gttaactgaa agcactgctt aggcagtgct
    36661 tttttgtttt cattcatcag agaaaatgat gtttccgcgt cttgatccag gctatagtcc
    36721 ggtcattgtt atcttttaaa tgttgtcgta atttcaggaa attaacggaa tcatgttcat
    36781 acgcgctccc aattctggac gtaagctcct gcttacctgc attgttgcag gcgtgatgat
    36841 tgcgatactg gtgagctgcc ttcagttttt agtggcctgg cataagcacg aagtcaaata
    36901 cgacacactg attaccgacg tacaaaagta tctcgatacc tattttgccg acctgaaatc
    36961 cactactgac cggctccagc cgctgacctt agatacctgc cagcaggcta accccgaact
    37021 gactgcccgc gcggcgttta gcatgaatgt ccgtacgttt gtgctggtaa aagataaaaa
    37081 aacattctgt tcatctgcga ctggtgagat ggacattcca ctcaatgaat tgattccggc
    37141 gctcgacatt aataaaaacg tcgatatggc gatcttaccc ggtacgccga tggtgccgaa
    37201 caaacccgca atcgtcatct ggtatcgcaa ccctttgctg aaaaatagcg gcgtctttgc
    37261 cgcgctgaat ctcaacctga cgccttcact cttttatagt tcacggcagg aagattacga
    37321 tggcgtcgcc ctcattattg gcaatactgc gctatctacc ttttcttcac gtttgatgaa
    37381 cgttaatgaa ttaaccgaaa tgccggtccg tgaaactaaa attgcgggca ttcctctgac
    37441 cgttcggctt tatgcggatg actggacatg gaacgatgtg tggtacgcat ttttactggg
    37501 cggcatgagt ggaactgtcg ttggcctgct ttgctattac ctgatgagcg tacgtatgcg
    37561 ccccggcaga gaaatcatga ccgccatcaa gcgcgaacaa ttttacgtgg cgtatcaacc
    37621 ggtggtggat acacaagctt tgcgggtaac gggcctggaa gtactgctac gctggcggca
    37681 tcctgtagcg ggagaaattc ccccggatgc cttcattaac tttgccgaat cgcaaaagat
    37741 gattgtgccg ctgactcagc acctgtttga gttaattgcc cgcgatgccg cagaattaga
    37801 aaaagtactg ccggtaggcg tcaaatttgg cattaacatt gcgccggacc atctgcacag
    37861 cgaaagcttt aaagcagata tccagaaact gctcacttcc ctgcccgctc accatttcca
    37921 gattgtgctg gaaattaccg agcgcgatat gctgaaagag caagaagcca cgcaactctt
    37981 tgcctggctg cactcggtcg gcgtagaaat tgctattgat gacttcggta ccgggcacag
    38041 cgcgcttatc taccttgagc gttttacgct cgattatctg aaaattgacc gtggatttat
    38101 caacgccatc ggtacggaaa cgatcacttc acccgtactt gacgcggtgc tgacgctggc
    38161 gaaacgtctc aatatgctga cggttgctga aggggtcgaa acgccggaac aggcgcgatg
    38221 gctaagcgaa cgcggcgtta atttcatgca aggctactgg attagtcgcc cgttaccgct
    38281 ggacgatttt gtccgctggc tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac
    38341 gtcccttatt attcatagtg aaagcatgcc ggattgcggc taatgatgag taaaaggaaa
    38401 tccgttgcag atgattgtgc gcatactgct gctgtttatc gctctgttca cctttggtgc
    38461 gcaagcgcag gctatcaagg aaagctatgc ctttgccgtg ctgggcgaac cccggtacgc
    38521 ctttaatttc aaccattttg attatgtgaa ccccgccgcg ccaaaaggtg ggcagataac
    38581 gttgtcagcc ctcggcacct tcgataattt caaccgctat gcactgcgcg gcaacccggg
    38641 cgcacgcacc gagcagctgt acgacacgct atttacgact tccgatgacg aaccaggctg
    38701 ttattacccg ctgattgctg aaagcgcacg ctatgctgac gattattcct gggtggaggt
    38761 cgctattaat ccgcgcgccc gttttcatga tggttcttcc attactgccc gcgatgtaga
    38821 gtttactttt caaaaattta tgactgaagg cgtgccgcaa tttcgtctgg tctacaaagg
    38881 caccaccgtc aaagccattg cgccgttaac cgtgcgcatt gagttagcta aacccggcaa
    38941 agaagatatg ctgagtctgt tttcgctgcc ggtatttcca gaaaagtact ggaagaatca
    39001 caaacttagc gacccgctcg ccacgcctcc gcttgccagt ggtccgtacc gaattacgtc
    39061 ctggaaaatg gggcaaaata ttgtctattc ccgcgtgaaa gattactggg cagcaaactt
    39121 accggtaaac cgtggacgct ggaatttcga caccattcgc tacgattatt acctcgatga
    39181 taatgtcgcc tttgaagcgt ttaaagccgg tgcctttgat ttgcgtatgg aaaacgacgc
    39241 caaaaactgg gccacgcgct ataccggtaa aaatttcgat aagaaataca tcatcaaaga
    39301 tgagcaaaag aacgaatcag cccaggatac gcgctggctg gcgtttaata tccaacgtcc
    39361 ggtattcagc gatcgccggg tgcgggaagc gatcactctc gcctttgact ttgaatggat
    39421 gaacaaagcg ttgttttaca atgcctggag tcgcacgaac agttattttc agaataccga
    39481 atacgcggcc agaaattacc ccgacgccgc ggagctggtg cttctggcac caatgaaaaa
    39541 agatctaccg ccagaagtct tcacgcaaat ctaccagccg ccggtatcca aaggcgatgg
    39601 ctacgatcgt gacaacctgt taaaagccga caaacttctc aacgaagcag gctgggtgct
    39661 gaagggtcag caacgcgtta atgccacaac gggtcagcca ctcagctttg aattattgct
    39721 tcccgcaagc agcaatagtc agtgggtatt gccgttccag cacagcctgc aacggctggg
    39781 tatcaacatg gacattcgca aagtggataa ctcgcaaatc accaaccgca tgcgcagtcg
    39841 cgactatgac atgatgccgc gcctatggcg ggcgatgccg tggcctagtt ccgatttaca
    39901 gatttcctgg tcatcggaat atatcaattc cacttataat gcccccggcg tgcaaagccc
    39961 ggttatcgac tcgctgatca accaaattat tgccgcgcag ggaaataaag aaaaattact
    40021 gccgttgggg cgagcactgg atcgcgtatt aacgtggaat tattacatgc tgccaatgtg
    40081 gtatatggcg gaagaccgtc tcgcctggtg ggataaattc tctcaacccg cagtgcgccc
    40141 tgtttacagc ctgggtatcg atacctggtg gtatgacgtt aataaagcgg ccaaactgcc
    40201 gtcagccaga caacagggag agtagatggg cgcttatctg attcgccgtc tgttgctggt
    40261 gatcccaacg ttatgggcga ttatcactat caactttttc atcgtgcaaa ttgcgcctgg
    40321 cggtccggtc gaccaggcca tcgccgccat tgagtttggt aatgccggag tattacccgg
    40381 cgcaggcggt gaaggtgttc gcgccagcca tgcgcaaacg ggcgtcggca atatcagcga
    40441 cagtaattac cgtggcggac gcggattaga tccagaagtg atcgccgaga tcactcatcg
    40501 ctacggtttc gataagccga tccacgaacg ttacttcaaa atgctctggg actacatccg
    40561 ctttgatttt ggcgatagcc tgtttcgcag cgcctcggtg ctgacgctga ttaaagacag
    40621 tctgccggtt tccatcaccc tcggattgtg gagcacgctg attatctatc tggtgtcgat
    40681 tccgttaggc attcgcaaag ctgtttataa tgggagccgc tttgacgtct ggagtagcgc
    40741 atttatcatc atcggctacg ccattccggc ctttttgttt gccatcctgc tgattgtctt
    40801 cttcgcgggc ggcagctatt tcgacctgtt ccctctacgc ggcctggttt ccgctaactt
    40861 tgattcgctg ccgtggtatc agaaaatcac cgattatctg tggcatatca cgctgccggt
    40921 gctggcgaca gtgattggcg gctttgcggc gctgaccatg ctgacaaaaa actcattcct
    40981 tgatgaagtg cgcaagcaat acgtggtgac cgcccgtgcg aaaggggtaa gtgaaaaaaa
    41041 tattctctgg aaacatgtgt tccgcaacgc catgctgctg gtgattgccg gttttccggc
    41101 gacgtttatc agcatgtttt ttaccggctc gctgctgatt gaagtaatgt tttcgctcaa
    41161 tggtcttggc ttactgggct acgaagcgac cgtctcgcgc gattatcctg taatgtttgg
    41221 tactttgtat attttcaccc tgattggcct gctgctgaat attgtcagtg atatcagcta
    41281 tacgctggtc gatccgcgta tagattttga gggacgttaa tgtcgcgact cagccccgtc
    41341 aatcaggccc gttgggcgcg ttttcgtcat aaccgtcgcg gctactggtc gttatggatt
    41401 ttcctcgtct tgtttggttt gagtttgtgt tctgaactta tcgccaacga taaaccgttg
    41461 ctggtgcgtt atgacggcag ttggtatttc ccgttgttga aaaactacag cgaaagcgat
    41521 tttggcggtc cgctggcaag tcaggctgat tatcaggacc cgtggctgaa acaacggctg
    41581 gaacataacg gctgggtact gtgggcaccg attcgctttg gcgctaccag
//
LOCUS       DAYPHN010000021        40881 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000021 DAYPHN010000000
VERSION     DAYPHN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.021"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(39..920)
                     /gene="htpX"
                     /locus_tag="ACG0BW_001140"
     CDS             complement(39..920)
                     /gene="htpX"
                     /locus_tag="ACG0BW_001140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFK7258130.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(1112..3160)
                     /gene="prc"
                     /locus_tag="ACG0BW_001141"
     CDS             complement(1112..3160)
                     /gene="prc"
                     /locus_tag="ACG0BW_001141"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFK7258131.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(3180..3878)
                     /gene="proQ"
                     /locus_tag="ACG0BW_001142"
     CDS             complement(3180..3878)
                     /gene="proQ"
                     /locus_tag="ACG0BW_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFK7258132.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(3975..4472)
                     /gene="msrC"
                     /locus_tag="ACG0BW_001143"
     CDS             complement(3975..4472)
                     /gene="msrC"
                     /locus_tag="ACG0BW_001143"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFK7258133.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            4602..5885
                     /gene="yebS"
                     /locus_tag="ACG0BW_001144"
     CDS             4602..5885
                     /gene="yebS"
                     /locus_tag="ACG0BW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFK7258134.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRGWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5854..8487
                     /gene="yebT"
                     /locus_tag="ACG0BW_001145"
     CDS             5854..8487
                     /gene="yebT"
                     /locus_tag="ACG0BW_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFK7258135.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            8567..10006
                     /gene="rsmF"
                     /locus_tag="ACG0BW_001146"
     CDS             8567..10006
                     /gene="rsmF"
                     /locus_tag="ACG0BW_001146"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFK7258136.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTASYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGIRNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALCPGGTLVYSTCTLNQEENEAVCRWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            10124..10360
                     /gene="yebV"
                     /locus_tag="ACG0BW_001147"
     CDS             10124..10360
                     /gene="yebV"
                     /locus_tag="ACG0BW_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFK7258137.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            10465..10656
                     /gene="yebW"
                     /locus_tag="ACG0BW_001148"
     CDS             10465..10656
                     /gene="yebW"
                     /locus_tag="ACG0BW_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFK7258138.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(10657..11313)
                     /gene="pphA"
                     /locus_tag="ACG0BW_001149"
     CDS             complement(10657..11313)
                     /gene="pphA"
                     /locus_tag="ACG0BW_001149"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFK7258139.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFEPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVFAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(11709..12050)
                     /gene="yebY"
                     /locus_tag="ACG0BW_001150"
     CDS             complement(11709..12050)
                     /gene="yebY"
                     /locus_tag="ACG0BW_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFK7258140.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVTSGKTKAQPISVIQIDDPNNRGEKMSL
                     APFIERAEKLC"
     gene            complement(12063..12935)
                     /gene="copD"
                     /locus_tag="ACG0BW_001151"
     CDS             complement(12063..12935)
                     /gene="copD"
                     /locus_tag="ACG0BW_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFK7258141.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(12939..13313)
                     /gene="yobA"
                     /locus_tag="ACG0BW_001152"
     CDS             complement(12939..13313)
                     /gene="yobA"
                     /locus_tag="ACG0BW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFK7258142.1"
                     /translation="MTSTARTLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            13452..13682
                     /gene="holE"
                     /locus_tag="ACG0BW_001153"
     CDS             13452..13682
                     /gene="holE"
                     /locus_tag="ACG0BW_001153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFK7258143.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            13784..14440
                     /gene="yobB"
                     /locus_tag="ACG0BW_001154"
     CDS             13784..14440
                     /gene="yobB"
                     /locus_tag="ACG0BW_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFK7258144.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            14464..15126
                     /gene="exoX"
                     /locus_tag="ACG0BW_001155"
     CDS             14464..15126
                     /gene="exoX"
                     /locus_tag="ACG0BW_001155"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFK7258145.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(15123..17183)
                     /gene="ptrB"
                     /locus_tag="ACG0BW_001156"
     CDS             complement(15123..17183)
                     /gene="ptrB"
                     /locus_tag="ACG0BW_001156"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFK7258146.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAVYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     PPED"
     gene            complement(17340..17765)
                     /gene="tnpA"
                     /locus_tag="ACG0BW_001157"
     CDS             complement(17340..17765)
                     /gene="tnpA"
                     /locus_tag="ACG0BW_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc46 family
                     transposase"
                     /protein_id="HFK7258147.1"
                     /translation="MSNHDDLLAGFLRKRHSVSKLVVHLIFTTKYRRKLFDSQMIAQL
                     REAFGSAAAKLECEIIEMDGEPDHVHLLVAYPPKLAVSVMVNNLKSVSSRLLRQQNAH
                     LRMQSKTGLLWSRSYFVCSTGEATIETLRAYVQSQSTPD"
     gene            17822..18991
                     /locus_tag="ACG0BW_001158"
     CDS             17822..18991
                     /locus_tag="ACG0BW_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFK7258148.1"
                     /translation="MLRATKVRIYPTPEQAEFINAQFGAVRFAYNKALHIKKHSYQRH
                     RVNLNPRKDLKPLLAVAKKSRRYGWLKEYDSIALQQAVINLDVAFSNFFNPKLKAGKP
                     TFKSKHGRQSSYHCVGIKVLDGAIKIPKILPIEARLHREITGMLKSITLSRSATGKYY
                     ASLLCDDGMEAPAKPALISNVTGLDMGLAHFVIKSDGNKIASPRHLINASRNLRRKQK
                     ALSRKKKGNANRRKARIQLAAVHERVASARADFQHKLSRAIVDENQAVIVETLKTANM
                     MKNHHLARAIGDAGWSGFITKLEYKATEKGVHLVKLDQWFASSKTCHCCGHKMSEMPL
                     HKRIWQCPECGVEHDRDINAALNIKRKGILELQAAGLVVSAHGGQHKSVTQTVAA"
     gene            complement(19155..19814)
                     /gene="yebE"
                     /locus_tag="ACG0BW_001159"
     CDS             complement(19155..19814)
                     /gene="yebE"
                     /locus_tag="ACG0BW_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFK7258149.1"
                     /translation="MANWLNQLQSLLGQSSSSASSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDARERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(20141..20497)
                     /gene="yebF"
                     /locus_tag="ACG0BW_001160"
     CDS             complement(20141..20497)
                     /gene="yebF"
                     /locus_tag="ACG0BW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFK7258150.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(20564..20854)
                     /gene="yebG"
                     /locus_tag="ACG0BW_001161"
     CDS             complement(20564..20854)
                     /gene="yebG"
                     /locus_tag="ACG0BW_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFK7258151.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20988..22166
                     /gene="purT"
                     /locus_tag="ACG0BW_001162"
     CDS             20988..22166
                     /gene="purT"
                     /locus_tag="ACG0BW_001162"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFK7258152.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFRKAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAAQIKVQA
                     "
     gene            complement(22221..22862)
                     /gene="kdgA"
                     /locus_tag="ACG0BW_001163"
                     /gene_synonym="eda"
     CDS             complement(22221..22862)
                     /gene="kdgA"
                     /locus_tag="ACG0BW_001163"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFK7258153.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(22899..24710)
                     /gene="edd"
                     /locus_tag="ACG0BW_001164"
     CDS             complement(22899..24710)
                     /gene="edd"
                     /locus_tag="ACG0BW_001164"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFK7258154.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(24945..26420)
                     /gene="zwf"
                     /locus_tag="ACG0BW_001165"
     CDS             complement(24945..26420)
                     /gene="zwf"
                     /locus_tag="ACG0BW_001165"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFK7258155.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            26758..27627
                     /gene="hexR"
                     /locus_tag="ACG0BW_001166"
                     /gene_synonym="yebK"
     CDS             26758..27627
                     /gene="hexR"
                     /locus_tag="ACG0BW_001166"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFK7258156.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            27755..29197
                     /gene="pyk"
                     /locus_tag="ACG0BW_001167"
     CDS             27755..29197
                     /gene="pyk"
                     /locus_tag="ACG0BW_001167"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFK7258157.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(29329..30300)
                     /gene="lpxM"
                     /locus_tag="ACG0BW_001168"
                     /gene_synonym="msbB"
     CDS             complement(29329..30300)
                     /gene="lpxM"
                     /locus_tag="ACG0BW_001168"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFK7258158.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(30420..31742)
                     /gene="mepM"
                     /locus_tag="ACG0BW_001169"
     CDS             complement(30420..31742)
                     /gene="mepM"
                     /locus_tag="ACG0BW_001169"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFK7258159.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(31758..32690)
                     /gene="znuA"
                     /locus_tag="ACG0BW_001170"
     CDS             complement(31758..32690)
                     /gene="znuA"
                     /locus_tag="ACG0BW_001170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFK7258160.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTEKQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            32769..33524
                     /gene="znuC"
                     /locus_tag="ACG0BW_001171"
     CDS             32769..33524
                     /gene="znuC"
                     /locus_tag="ACG0BW_001171"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFK7258161.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPDTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELGCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            33521..34306
                     /gene="znuB"
                     /locus_tag="ACG0BW_001172"
     CDS             33521..34306
                     /gene="znuB"
                     /locus_tag="ACG0BW_001172"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFK7258162.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(34458..35468)
                     /gene="ruvB"
                     /locus_tag="ACG0BW_001173"
     CDS             complement(34458..35468)
                     /gene="ruvB"
                     /locus_tag="ACG0BW_001173"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFK7258163.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(35477..36088)
                     /gene="ruvA"
                     /locus_tag="ACG0BW_001174"
     CDS             complement(35477..36088)
                     /gene="ruvA"
                     /locus_tag="ACG0BW_001174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFK7258164.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(36227..36292)
                     /gene="yobI"
                     /locus_tag="ACG0BW_001175"
     CDS             complement(36227..36292)
                     /gene="yobI"
                     /locus_tag="ACG0BW_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFK7258165.1"
                     /translation="MCIFITHFFAEYVIFKYLLPI"
     gene            36363..36965
                     /gene="yebB"
                     /locus_tag="ACG0BW_001176"
     CDS             36363..36965
                     /gene="yebB"
                     /locus_tag="ACG0BW_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFK7258166.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFMVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKKALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(36967..37488)
                     /gene="ruvC"
                     /locus_tag="ACG0BW_001177"
     CDS             complement(36967..37488)
                     /gene="ruvC"
                     /locus_tag="ACG0BW_001177"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFK7258167.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(37523..38263)
                     /gene="yebC"
                     /locus_tag="ACG0BW_001178"
     CDS             complement(37523..38263)
                     /gene="yebC"
                     /locus_tag="ACG0BW_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFK7258168.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(38292..38744)
                     /gene="nudB"
                     /locus_tag="ACG0BW_001179"
     CDS             complement(38292..38744)
                     /gene="nudB"
                     /locus_tag="ACG0BW_001179"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFK7258169.1"
                     /translation="MTDMTYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(38737..40509)
                     /gene="aspS"
                     /locus_tag="ACG0BW_001180"
     CDS             complement(38737..40509)
                     /gene="aspS"
                     /locus_tag="ACG0BW_001180"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFK7258170.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            40819..>40881
                     /locus_tag="ACG0BW_001181"
     CDS             40819..>40881
                     /locus_tag="ACG0BW_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258171.1"
                     /translation="MLELNAKTTALVVIDLQEGIL"
BASE COUNT         9852 a        10608 c        10144 g        10277 t
ORIGIN      
        1 gcgtcgatca ggacgcgctt tttagtattt acttcatatt acttcaggta ttcacccgta
       61 cgcagagctt caatacgttt atccagcggc gggtgagtca tgaacaactc actgagcgat
      121 ttcgacttac cgttaatgca gaaagccatc atgctggttg cttcttgcgg ttcatagctg
      181 gttttcaggc gctgcaatgc agcaatcatt ttctcgcgac caaccagttt tgctgaaccc
      241 gcatcggcgt ggaattcacg atgacgcgag aaccacatgg tgataatgct cgccagaata
      301 ccaaacacca gttccagaac cgttgcaacc gcaaagtaga tcagcgggtt gccgttgctc
      361 tcttcacctt catcacgatt tccgcccata aaacccgcgg caagctgcgc cagaatacgg
      421 gaaataaaga taacgaaggt gttcaccacg ccctgaatca gcgtcatggt gaccatatca
      481 ccattggcga tgtggctgat ttcgtgagca attaccgcct cggcttcatc cgggctcatg
      541 ttctgcagca aaccggtgct gacagcaacc agagaggcat cacggcgcgc accggttgca
      601 aaagcgttga tgtccggcgc atggtagata gccacttgcg gcatagcgat ccccgcctga
      661 cgagcctggg ttgctacagt attgaccagc caacgttccc tttcgttacg cggttgctcg
      721 atcacttccc cgccaacaga tcgtaatgcc atccatttgg acatcagaag cgaaacgaag
      781 gaaccaccaa aaccgaacag caaggccatg atcatcagcc cctgaacgct gctcgactgt
      841 atccctgtca ggctcagtac cagcccgaaa acgaccatta cggccaggtt cgttagcagg
      901 aagagcgcga ttcgcatcat aattttcttt ttacctcagt ttaacaaaac gcaatatgcg
      961 atacccacat cgtatgggtt acgcgactat tttcaagtct ggatagtgcg taagtcacca
     1021 gaaagacaca actttacatt ttgtagcatc tgatttacgg catcttgccg ctgttaaaaa
     1081 atcaggcaca atttcttgtg cctgattgat attacttgac gggagcgggt tgttccgcgg
     1141 gtctggcttt ttcaagcttc gccagatcga gtgcgatatt caccgtctca tccagataag
     1201 gatccggctc ctggtaatct ttcggtagat catccagttt cttcaactcc ggtttacctt
     1261 cgcgtttaaa gcgttcgttc aaacgcgcca gacgcgtcgc atcatcttca ttattctctt
     1321 tctcacgcac agcgtaattc agagaaacga tattgcgctt gtccttcata gcgttgaagc
     1381 gcgcgatatc cttcatgatg ttctggaact caggatcttt cgcgatacgc gcattatgtt
     1441 ccttcagcag ctccggtcca aaggccgtca aatctcccga tttcacatag gtcgcggcat
     1501 cgatgctatc ccacggcagc gcgttatctt cgaatttctc accggtttcc gtttcttcat
     1561 tacccgtcgg catgatgatg tctggcgtta cgcctttacg ttgcgtactg ccgccgttaa
     1621 cgcgatagaa tttctggatc gtgtactgca cagaacccag cgctggccat tcaggacgta
     1681 acatctgatc gtaaatacgg ttcaatgaac ggtattgctg aacggtgcct ttaccaaacg
     1741 tcggttcacc cacaaccagc gcacgaccgt aatcctgcat ggcagcagca aagatttctg
     1801 aagccgaagc actgaagcgg tcaaccagca ccaccagcgg gcctttatag aaaacctgcc
     1861 cgtcggtatc gctgtcttca cgaactttac cgttgttatc acggacctga acaatcggac
     1921 cggatggaat gaacagaccg gagagcgata ccgcttcggt caacgcccca ccgccattgc
     1981 tacgcaggtc gatgatgaca ctgctgacat tctgtttttc cagtttctgc agttgcactt
     2041 tgacatcgtc tgtcaaaccc acatagaagc ccggaatatc cagcacgccg actttctctt
     2101 taccgacggt cttcaccgac attttaaccg cgcggtcttc gagacgaata cgttcacggg
     2161 tcaacgttac agtacgggtc ttggtccctt taccagcagg taaaatttcc agacgaactt
     2221 tactgccctt cggcccttta attaaggcaa ccacatcatc aagacgccag ccaatcacgt
     2281 caaccatcgg cttgcctgtt tgaccaacac cgacaatttt gtcaccaacg ctgatagctt
     2341 tactcttcgc tgccggacca cctgccacca tcgaattgat aacggtgtag tcatcatcca
     2401 tttgcagaac tgcgccaata ccttccagcg acaaactcat ttcagtgttg aactgttcgg
     2461 tattacgcgg ggaaagatag ttggtatgcg ggtcgatttc acgcgcaaac gccgtcattg
     2521 ccagcgagaa aacatcttcg ctgttggttt gcgccagacg acgaatggca aatttgtagc
     2581 ggcgagtcag ggtttcacga atttctttat ccgtttttcc tgccagcttc aggcttaact
     2641 cgtcgaattt gactttactg tcccacagcg cgttcaactc agcctcgttt ttcggccatg
     2701 gcgctttgct gcggtcaagg ttataagtgt cgttgccggt aaaatccatc ggcttttcca
     2761 gtaccgacaa agcgtactgg taacgctcaa aacggcgctt ttgcgccaga ttgtagagat
     2821 cgtagaaaac gtcgagtttt cctgaacgca gttcatcacc taactcggtt ttctttttcg
     2881 cgaactgttc aacatcgctt gccagcagca cgttgtggct gtaatcgagc agattcaggt
     2941 agcggtcaaa gattttggcc gaaaatgcct gatcgaggtc gaactggcga taatgagaac
     3001 gggtgaagcg cgacgttacg cgctcactta ccgtcgcatg ctgcgtctct tcctttaata
     3061 ccggaatttg atcagcacgc gtgatatctt ctacagcgaa ggtctggcct gctattgcaa
     3121 gcaggccagc taacgcggta agcctaaaaa acatgttcat gcctggcccg gcctccgttt
     3181 cagaacacca ggtgttctgc gcgcacaatc aaagacatac ccgaattcag ctggacgcgg
     3241 acgccgtctt tggtgatttc taatacggtg gcatccatcg cgttttgacc cgctttcacc
     3301 ttcagggctt gtccgacagt cagagctgaa atgtcagaaa ccggggtgtg ctgttcttcg
     3361 cgaggtgctt ttactgtttt tggcgctttc tctaccggct tttgcgcacg aggtttacgt
     3421 tcagcgcctt ctttgcggcg tggcgtggtc ggacgtggct tgcgttcgcg gcgcggtgcg
     3481 tcttctttct caccagcagc tgcggcagct tcgcgttttt tcgcttgctg ctcagcacgc
     3541 tgtgcctgaa cacgcgcttt cgcttcttca agctgcttgc gagcatgctc tacatgttgc
     3601 tcgtccagct caccgcatgg gttgccgtca agatcgacac gcgttgcgcc gggtttaaca
     3661 ccgtaaagat aacgccagct cgaagtgtag agacgtaaag cggatcgcaa ttgcgttttg
     3721 ctcaggttca tttccccggc aacacgatcg accaaatcct gaaaaatacc gattttcagc
     3781 ggacgcgctt caccttccgc actgaaacag tggggaaaac gttcggccag aaacgcgatt
     3841 acttctttac tgctattcaa cttaggttga ttttccatga aatttcctga ttacaacgga
     3901 cgtagccaac aagcgcaggc atgaacaggc gtcattataa tgacgctacc agtaaatgct
     3961 acgttatccg ttgattatcc tgcgacgctc gcaaagaatt ttttgtaatc cgtcgttgca
     4021 agcacttttt caagctgtgc cacaagctga cgtaagcctt gctcgtcctc gtctgtaaag
     4081 cgaccgaaga cggtactatc gatgtcgaga acaccaataa tctgattttt caccaccagc
     4141 ggcagaacaa tttcagaatt actcgccgca tcacaggcaa tatgcccgtc aaacgcgtga
     4201 acatcctcga tacgctgcac ttgattgcgg gcaaccgcag tgccgcacac cccgcgcccg
     4261 acgggtatcc ggacacaggc aattttgccc tgaaatggtc cgagcaccag tgtatcgtcc
     4321 tcaagcaaat aaaaacctgc ccagtttacg tcagtgagac gctcatataa caacgcactg
     4381 gtgttcgcaa gcgttgccag aaaactggtt tctcctgcca tcagcgcgtt aaagtcgcga
     4441 tttaaatccg cgtaaaattc tattttgttc attatataat cacttggttg tcttacctgg
     4501 aactgccagc ctattaaaat aagcattaaa tgcgttaatg ctcaagatca ttcccatcat
     4561 gggttaagat taatgttaat tcttattaca tttggcacgt catggctctt aacacaccac
     4621 aaattacgcc gacaaaaaag ataacagtga gggctatcgg tgaggaactg ccgcgtggtg
     4681 attaccaacg ttgcccgcaa tgtgacatgc tgtttagcct gcccgagata aattctcatc
     4741 aaagtgccta ttgtccgcgc tgtcaggcaa aaattcgtga cgggcgcggc tggtcgctaa
     4801 cgcgcctggc ggcaatggcc ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac
     4861 cgctgttgca tatctggctg ttaggcattc gtattgacgc caacgttatg caaggcatct
     4921 ggcaaatgac caaacagggc gatgcgataa cggggtcgat ggtctttttc tgcgttatcg
     4981 gtgcccccct cattctggtg acctccatag cttatttatg gtttggtaac cgactgggaa
     5041 tgaatttacg tccggtactg ctgatgcttg aacgacttaa agagtgggta atgctggata
     5101 tctacctggt cggcattggc gttgcttcta taaaggtaca ggattatgcc catatccagg
     5161 cgggtgtcgg cttgttctct tttgtggcgt tggtgatttt aacgacggtg acgttgtcac
     5221 atctcaatgt cgaagaactg tgggagcgat tttatccaca gcgccccgct acgcgtagag
     5281 acgagaaact ccgtgtctgt cttgggtgcc attttaccgg ctatcctgat cagcgtggtc
     5341 gctgcccgcg ttgccatatc ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct
     5401 gggcggcgct gttagcgtca atcgttttgt tgttacctgc caacctgttg cctatttcta
     5461 tcatttatct gaatggtggc cggcaggaag atacaattct ttccgggatt atgtcgctgg
     5521 caagtagcaa cattgcggtc gccggaatcg tgtttatcgc cagtattctg gtaccgttta
     5581 ctaaagtgat cgtcatgttc actttactgt tgagtattca ttttaaatgc cagcaaggtt
     5641 tacgcacacg cattctgtta ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg
     5701 acctgtttgt catatcttta accatgtcgc tgattaatcg cgatcagatc ctcgctttta
     5761 ctatgggacc ggctgcgttt tatttcggcg cagcggtaat tttgactatt cttgctgtgg
     5821 aatggctgga cagccgctta ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg
     5881 actgaagcgc agattaaaaa taaacgccgt atctcacctt tctggctgct gccattcatc
     5941 gcgctaatga ttgccggttg gctgatttgg gatagttatc aggaccgggg taataccgtc
     6001 accatcgact ttatgtcggc ggatggtatt gttccaggcc gtacgcctgt tcgttatcag
     6061 ggcgttgaag tcggaactgt gcaggatatc agcctgagcg acgatcttcg taagattgaa
     6121 gtcaaggtca gcatcaagtc cgatatgaaa gatgcgctgc gcgaagagac acagttctgg
     6181 ctggtgacgc caaaggcctc gctggcaggt gtctccgggc tggacgccct cgtcggtggt
     6241 aactatatcg gcatgatgcc aggtaaaggt aaagagcagg atcactttgt cgcactcgat
     6301 acccaaccga aatatcggct ggacaatggc gatctgatga tccacctgca agcccccgat
     6361 ctcggttcgc tgaacagcgg ttcattggtc tatttccgca agatcccggt cggaaaagtc
     6421 tacgactatg ccatcaatcc caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg
     6481 cgttttaccg atctggtgaa aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc
     6541 aacgtcagta tcagtggcgc gaaggtgaaa ctggaaagcc tggcggcact ggttaacggt
     6601 gcgattgcct tcgattcacc tgaagagtcg aaacctgccg aggcggaaga tacctttggt
     6661 ctgtatgaag acctggccca tagccagcgt ggcgtaataa tcaaactgga actgccgagt
     6721 ggggccggat taaccgccga ctcgacgccg ttaatgtatc aggggctgga agtcggacag
     6781 ctgactaaac tggatttaaa tcctggtggt aaagtcactg gggaaatgac cgttgatccc
     6841 agcgtcgtca ccctgcttcg tgaaaatacc cgcatcgaat tacgtaatcc gaaattatcc
     6901 cttagcgatg ccaatctcag cgccctgctg accggcaaaa ccttcgagtt ggtgcccggc
     6961 gatggcgagc cacgcaaaga gttcgttgtt gtgccaggcg aaaaagccct actgcaagag
     7021 ccggatgttc tgacactgac cctgaccgcg ccggaaagtt acggtattga tgctggtcag
     7081 ccgctcattc ttcacggcgt gcaggtaggc caggttattg atcgtaaact caccagcaaa
     7141 ggcgtcacct ttaccgtcgc catcgagcct cagcatcgcg aactggtaaa aggcgatagc
     7201 aaatttgtcg tcaacagccg tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc
     7261 ggtgccagcg cctcagaatg gattaacggc gggatacgta ttctgccagg cgataaaggc
     7321 gagatgaaag ccagctatcc actgtatgcc aatctggaaa aagcgctgga gaacagcctt
     7381 agcgatttac ccaccacaac cgtgagtttg agtgcagaga cgctgccgga tgtgcaggca
     7441 ggatcggtag tgctgtaccg taaatttgaa gttggtgaag ttattaccgt gcgtccgcga
     7501 gctaacgcgt ttgatatcga tctgcatatt aagccggagt atcgcaacct tctgaccagc
     7561 aacagcgtgt tctgggcaga aggtggggcg aaagttcagc tgaatggtag tggtctgact
     7621 gtacaggcat ccccgctctc cagagcatta aagggggcta ttagcttcga taacctcagc
     7681 ggtgccagcg ccagtcagcg taaaggcgac aagcgtattc tgtatgcttc cgaaacagca
     7741 gcccgtgcgg ttggcgggca gattacgctt cacgctttcg atgccggaaa actggcggtc
     7801 gggatgccaa ttcgctatct cggtattgat atcgggcaaa tccagacgct ggatctgatt
     7861 accgcgcgca atgaagtaca ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt
     7921 gctcgcggtg gtacgcgctt ctcagttgtc acaccgcaaa tttcggcagc tggcgttgag
     7981 catcttgata ctatcctcca gccgtatatc aacgtcgaac caggacgggg caaccctcgc
     8041 cgcgactttg aattacaaga ggccaccata actgattcgc gttacctgga tggcttaagc
     8101 attattgttg aagcgccgga agccggttcg ttaggcatcg gtacgcctgt gctattccgt
     8161 ggtctggaag tcggtacggt taccggaatg acgctgggga cattgtcaga tcgcgtgatg
     8221 attgcgatgc gcatcagtaa acgctatcaa cacctggtgc gtaacaattc cgtcttctgg
     8281 ttggcatcgg gttacagtct ggactttggt ctgacgggcg gcgtcgtgaa aaccggcacc
     8341 tttaaccagt ttatccgtgg cggcatcgcc ttcgctacgc ctccgggtac gccgctggca
     8401 ccgaaagccc aggaaggcaa gcacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt
     8461 gaatggggaa ctgcgcttcc caaataatgc ccactgctcc ggcgtgcctg cgccggagcg
     8521 tttatgctaa actgcgcgcc tgtttttttg ccagtggtac atgctcgtgg cccaacacac
     8581 cgtttatttc ccggacgcct ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc
     8641 atttgatgat tttcttgccg cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac
     8701 gctgaaaatc tctgttgctg atttcctgca attaaccgct tcttatggct ggacgcttac
     8761 gccaattccg tggtgtgaag aaggtttctg gattgaacgc gacgatgaag atgcattgcc
     8821 gttgggtagt accgccgagc atttaagtgg cctgttttat attcaggaag ccagttcaat
     8881 gttgcccgtc gccgccttgt ttgctgacgg taatgcacca cagcgggtga tggatgtcgc
     8941 tgccgcgccc ggctccaaaa cgacgcaaat tgccgcgcgg atgaataacg aaggggcgat
     9001 ccttgccaat gagttttccg ccagtcgggt aaaagtgtta catgccaata tcagccgctg
     9061 tggcatccgt aatgttgcgc tcacacattt tgatggccgc gtgtttggtg cggcgttgcc
     9121 agaaatgttc gatgccattt tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa
     9181 agatcccgat gcgctaaaaa actggtcacc agaaagcaat caggaaatcg cagctaccca
     9241 acgggaactg atcgacagcg cctttcatgc attatgccct ggcggtacgc tggtttactc
     9301 gacctgtacc ttaaaccagg aagaaaacga agccgtttgc cggtggctga aagagactta
     9361 ccccgacgca gtagagtttt taccgcttgg cgatctcttc cctggtgcaa acaaggcgct
     9421 gaccgaggaa ggctttttgc atgttttccc acaaatttac gactgcgaag gcttcttcgt
     9481 tgctcgtctg cgtaaaactc aggcgattcc cgccttaccc gccccaaaat acaaagtggg
     9541 caatttcccg ttcagcccgg tgaaagatcg cgaagctgga caaattcgtc aggcggctgc
     9601 aggtgttggc ttaaactggg atgaaaacct gcgcctctgg cagcgtgaca aagaactgtg
     9661 gttgttcccg gtgggcattg aagccctgat cggtaaagtc cgattttctc ggttggggat
     9721 taaacttgcc gagacgcaca acaaaggtta tcgctggcag catgaagcgg ttattgctct
     9781 tgcctcaccc gataatatga atgcattcga gctgacgccg caggaagcgg aggagtggta
     9841 tcgcgggcgc gatgtttacc cgcaagccgc gccagtggcg gatgatgtat tggttacttt
     9901 ccagcatcaa ccgattggtt tagccaaacg gattggttcg cgactgaaaa acagctatcc
     9961 gcgtgaactg gtgcgcgatg ggaaactttt taccggtaac gcctgacagc gcacaaaaaa
    10021 agcgcacttt ttgactggca cattcggctg cctcaactag gctgaaaaat ggtgcgatcg
    10081 gactggtcgt accataatcg gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg
    10141 cataggcgct tttgaaatcg acgacggcga attacacggt gaatcgccgg gtgatcgaac
    10201 gttaaccatt ccttgtaaat ctgaccccga tttatgtatg caactggatg cctgggatgc
    10261 tgaaaccagt atccctgccc tgctaaatgg cgaacactct gtcctttacc gtacccgtta
    10321 cgatcaacaa tctgatgcct ggattatgcg tcttgcctaa tccaaaaaga acccgtcggc
    10381 atggcgggtt atttgtcctg gttattcccc cgttgtaaaa tctctcctaa acttaacggt
    10441 acggcaccac acttcgggga tgaaatgttc gcgctggtac tttttgtttg ctacctggat
    10501 ggcggttgtg aagatattgt tgtggatgtc tacaacacgg aacagcagtg tctttattct
    10561 atgagcgatc aacggatccg ccatggcggt tgttttccaa tcgaggattt tatagatggt
    10621 ttctggcgac ctgcacagga gtacggtgat ttttaattat tgcaattgca caagagtcag
    10681 ttcgccccca aagacagcac cggtatcaat ataatgcagg ttgccaatat ccacgcgatg
    10741 gcgcaacggt gtatgaccaa accagaaatg atcagcacct gtaattccct gccctttttg
    10801 gcgttcacct aatcgcgagc ggctccacaa gacctgatgc aaatcaacgt ccttttgcca
    10861 ttcataaaca tcatctggat aatcggcatg agcaaaaaca tgtttgccgg tgcggctgtg
    10921 tacttcaaga ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg
    10981 ttgattatct gccagcgcaa taaaccagtc gccgccattc atcaaccata aagacatctg
    11041 ctgggatgcc agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca
    11101 atgttgttcc agtaactgca ggcaacgtaa actttgcggc ccacgatcga taacgtctcc
    11161 cactgagata agtaaatctc gccacggttc aaaaccacaa tgccataatt tgcggcgcaa
    11221 ctgctcaaga caaccgtgta tatcgcctga aagccagata tgtcgccatt gatgacccgc
    11281 aattctctga taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc
    11341 acataatctt aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg
    11401 gttgactata aagtcagcga agggaatgct tctggctttt aacagataaa aagagaccga
    11461 acacgattcc tgtattcggt ccagggaaat ggctcttggg agagagccgt gcgctaaaag
    11521 ttggcattaa tgcaggctta gttgccttgc cctttaagaa tagatgacga cgccaggttt
    11581 tccagtttgc gtgcaaaatg gtcaataaaa agcgcggtgg tcatcagctt aaatgttaaa
    11641 aaccgcccgt tctggtgaaa gaactgaggc ggttttttta ttggaaatca aaaggttatt
    11701 ttaggtaatt aacagagttt ttcagctcgt tctataaacg gtgccagact cattttttcg
    11761 ccgcgattgt taggatcatc aatctgaatc accgaaatgg gctgggcttt agtcttccca
    11821 ctggtaactt ccttttgtgc gatatcgttt aaaggatact gcacgagggt acttgggttg
    11881 atgacataca aagcattacc cggtcggcaa gtcagcatca cctcttcgcg attaaacgcc
    11941 catttgtctt tacccacttc aaaacggctg acggtaatca cctgcggtgc cgccagcgcc
    12001 gctgcagaac tggtgagtaa cagaaacgcc agaatacttt ttttcatcat tttcgtaatc
    12061 catcaaaaag gttcgagagt cgcaagcagg ctgatgatca ccagcactac cgcacctatc
    12121 gcccattcga gtttagtcat ccacacaaac cagggtgccg cacgatcttc atcctgcctc
    12181 atgcgcggta caagaacata acgattagcc agcgcaatga ccaccataat catcaccagg
    12241 atggctttga gcaataagag ttggccccag taagtcgtaa gcgtgggggg aaatccggta
    12301 atcaacaatg cattaagcac gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac
    12361 caggaaaaac gcatcagcgc ctgaatagcc tggtgtcgcc aacggccttt gatgagttgc
    12421 atacaccaga gcaccggcag taatccccca aaccacgccg ccgcacagat caggtgaatc
    12481 gcgtgattag tctgatggat tttcgccgtt accccttcat tcagcgtcgc atgcccgact
    12541 cccgccagca gaataaattg cgcggtagtg agcataaaca gcaaacgtgg catattccgc
    12601 ggttgcataa gggcgacgat caacgttacg agggcgagaa caatttgcca taaccagacg
    12661 ccaccaaact gcgtttgcaa aacagcctgc caaatgtttg gcgaaaatac atctgtccat
    12721 cccgttccca tcaacccgcc ctgaacggcg agcattgcag tggcactgat caaactccag
    12781 acggcagcgt gctgttgtaa acgcagaaaa cgcttcgtca gcaagcgacg aatcgttaag
    12841 ggagccagcc aggcaccgta cattgcgaag ccaaagacca gcatcagcga ggtgaaatgg
    12901 ataaatcgca gcgcgatcca ggtaaacgcc agcatgattt atttcacgct aaaggtgtag
    12961 tgtcctttcg ttttatgccc gtccaccgaa acaacatgcc agtcaacggt gtaggttccg
    13021 ggtttcagcg aatcggccag tggaacaatc agttgctttt ggtcctgctc attccgcttc
    13081 gccggtaatg ttttaatatt ttcgtttttt ggccccgtga tttttgcacc actgaatcct
    13141 gtttcaacgc cttccgagaa gtttaaagta attgcctgcg gggcagctgt cacttgcgcg
    13201 tttgccgcag gatactgatg cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac
    13261 gaagtggtca ggattgccag cgcgtagcga agggtgcgtg cagttgaagt catattatct
    13321 attccttttt gtaataactt ttttacagag cataaccttg tctaatgtct gagtcgagga
    13381 tcatcaattc cggcttgcca tccaggctca ctcttagtaa cttttgcccg cgaatgatga
    13441 ggagattaag aatgctgaag aatctggcta aactggatca aacagaaatg gataaagtga
    13501 atgtcgattt ggcggcggcc ggggtggcat ttaaagaacg ctacaatatg ccggtgatcg
    13561 ctgaagcggt tgaacgtgaa cagcctgaac atttgcgcag ctggtttcgc gagcggctta
    13621 tcgcccaccg tttggcttcg gtcaatctgt cacgtttacc ttacgagccc aaacttaaat
    13681 aaaacttata cagagttaca ctttcttaca taacgcctgc taaattatga gtattttcta
    13741 aaccgcaccc ataatttgca gtcattttga aaaggaagtc attatgtcgt tctggaaagt
    13801 tgcagcagcg caatatgagc ctcgtaagac ctcactgacc gaacaggtag ctcatcatct
    13861 ggagtttgtg agggccgccg ccaggcaaca atgtcagctg ttagtttttc catcgctttc
    13921 tttactgggg tgtgattatt cgcgacgtgc ccttcctgcc ccacccgatc tctcgctgtt
    13981 agacccgctt tgttatgctg caacgacctg gcgaatgacc atcattgccg gccttcccgt
    14041 tgaatataac gatcgcttta ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacacc
    14101 agggatctac catcagagtc atggtgcatg tctgggccgt cgttccagaa ccatcacggt
    14161 ggtcgatgaa caaccggaag gtatggatat ggacccgacc tgttcactgt ttacgacagg
    14221 gcaatgtctg ggagaacccg acctgctggc gtcggcccgt cgcttacaat ttttttcaca
    14281 tcagtactcg attgctgtgc tgatggcaaa tgcccgtggt aacagtgcac tatgggatga
    14341 acatggtcgt ctcatcgttc gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc
    14401 atcacagggt tggcaaggcg atatcattcc attacgctag gctttttcgg cctggagcat
    14461 gccatgttgc gcattatcga tacagaaacc tgcggtttgc agggagggat cgttgagatt
    14521 gcctctgttg atgtcattga cggaaaaatc gtcaacccca tgagccacct ggtgcgcccc
    14581 gatcgtccta ttagtccaca ggcgatggcg attcatcgca tcaccgaagc catggtcgcc
    14641 gataaaccgt ggattgaaga tgtgatcccg cactattacg gtagtgaatg gtatgtcgcg
    14701 cacaacgcca gctttgaccg ccgcgtactg cctgagatgc ccggtgagtg gatttgcact
    14761 atgaaactgg cccgtcgttt gtggccaggg atcaagtaca gcaatatggc gttatataaa
    14821 acacggaagc tcaatgtaca gacgccgccg ggcctgcatc atcaccgcgc gttgtatgac
    14881 tgttatatca ccgccgcgtt gcttatcgat attatgaaca cctccggctg gacggcagaa
    14941 cagatggccg atatcaccgg acgtccgtcg ttgatgacga ccttcacctt tggcaaatac
    15001 cgtggcaaag cggtttccga cgttgccgaa cgcgatccgg gctatctgcg ctggttattt
    15061 aataacctgg acagcatgag cccggagctg cgtttaacac tgaaacatta tctggaaaat
    15121 acttaatcct cagggggagc gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat
    15181 tccatcgcta cgccttcgta cgatttaaag cggccagatt taccgccatg gcctgagtcc
    15241 atgtcggtac agagcaataa aagatggtta tcggttttca gctcgcgcaa tttagccacc
    15301 cattttgccg gttcccaata ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa
    15361 tgcggataag cctgtgcggt gacgttgtca tacgggctgt agcttttcat gtactcgtaa
    15421 tattgcggat cctgcggatt cccccactct tcaaactcac cagtggtaag aggaattgat
    15481 tcatcaagca tcgttgttac aacatcaaca aacggtacct gggcgataac gccatggaat
    15541 aattccgggc gttgattaat tgcaacaccc atcaacatgc cccccgcact cccgcccatc
    15601 gcataacaaa gcgaaggaga gccatagccc agctttaaca atgcatcgca ggcatcaaga
    15661 taatcattaa acgtattttt cttcttcaga aacttaccgt cttcgtacca ttgttgcccc
    15721 agttcaccgc cgccgcgaac atggacaatg gcgtagacaa agccacgatc caacaaactc
    15781 aagcggctaa aactgaaatc ggcatcaata cttgcgccgt aagaaccgta gccatacacc
    15841 agtagcgggt tgtgtccttt gcgaaaatgt ttgcgatggt agaccagcga aaccggaact
    15901 tcgacgccat cacgggcgac tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa
    15961 ccaggaactt ccgtttgttt taatacacga cgctcaccgg tatccatatc cagttcaaac
    16021 agagtgtctg gtgtggtcat ggaagaataa ccataacgca atcgcgcggt ttcaggttct
    16081 ggattgtagg caatccaggt cacataggcc ggatcatcaa aggcaatgcc aatgacttcc
    16141 cgggtcttgc ggttgatttg gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc
    16201 agccagtcgg taaacagcgt aaacccttcc agcataatgt tatcgcgtgg cggaattaac
    16261 tcttcccact gttgctcatc acgcatacgg gtacggtata agccaaagtt tttgccgtgg
    16321 cggttggaac gcaaataaaa ccgatgctgg tagtgatcaa ggctgtattc gtgatctttg
    16381 cggcgcggca gaaaaacaaa cggctcggca tcggccattt ccgcgtccag caggcgaact
    16441 tcactggtgg tggcgctggc cagatgaatg actacataat gtttcgaggt cgttttatgc
    16501 aggctgacgt aataggtatc gtctttttct tcgtagatca gtttatcttg cgatgctggc
    16561 gtaccgatgg cgtgacgcca gacctgataa ggcagcagcg taactggatg cttgcgtaca
    16621 tagtagaaag tccaggagtc atttgcccag acaaagctgg gttcaacgtt atccagcagt
    16681 tccgggtacc agttacctgt ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa
    16741 agaaaatctt ctgccagcgc cataatggtg ttatcgggcg taatcgccat tccgcccatc
    16801 gaataaaact cactatgagc tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc
    16861 cactcttcgc tgaatgccga ttgacgctgg tagacagcat attcacagcc tggttcataa
    16921 atatgccgat agcggtagcc atttttgatg tagggagcag aaacttctct ttgcggaatg
    16981 cggtcgatga tttcctttaa gatgcgatcc tgcaaggctt gttgcgaggc catcacccga
    17041 tgaccgtaac tattttcttg ctgcaggtag tccaggactt ccggctgaga acgcgtatcg
    17101 tcccgcagcc agtagtaatt atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga
    17161 atgcgggcgg cttttggtag catgttattg ttctttctgg ttgaaacatc ttataagggt
    17221 ggcaaaactc accggagatg caagcgaaac agggtagtca ttgcttagcg aaaaacgccg
    17281 taaaccctcg cccaatgcgg gcggggatat aaggcgaaaa gcccgcaggg ctttaaagat
    17341 caatcaggtg ttgactgact ctgaacgtag gctctgagcg tttcaatcgt tgcctctccg
    17401 gtgctacaga caaagtacga tctcgaccac aaaagccccg ttttactctg catacgtaaa
    17461 tgcgcgttct gctggcgtag caaacgtgat gataccgatt ttaaattatt gaccatcaca
    17521 ctgaccgcca gttttggcgg gtaggcaacc agcaggtgta catggtctgg ctccccgtcc
    17581 atctcaataa tttcgcattc aagctttgcc gcagcggagc caaaagcctc acgcaactga
    17641 gcgatcatct gactgtcgaa tagtttgcgt cgatactttg tcgtaaaaat aaggtgcaca
    17701 accagcttac tgacactgtg tcgtttgcgg agaaatcccg ccagtaaatc atcatgatta
    17761 ctcaattgat atatttacct aaagtgttaa aataaaagca ttatatcaat gagcgctgat
    17821 tatgttaaga gcaacaaaag tacgcatcta cccgacacca gaacaggctg aatttatcaa
    17881 cgcccagttc ggtgcagtgc gttttgcgta caacaaagct ctgcatatca agaaacattc
    17941 ttaccagcgg caccgcgtaa atttaaaccc gcgtaaagac ctcaaaccac tgcttgcagt
    18001 ggcgaagaaa tcccgccggt atggatggct taaagaatat gattctattg cgttgcagca
    18061 ggcggtaatt aacctcgacg tggctttttc taactttttc aatccgaaac tgaaagcagg
    18121 taaacctacc tttaaaagta aacacggcag acaatcgagc tatcattgtg ttggcataaa
    18181 agtccttgat ggtgcgataa aaataccgaa aatcttgccg attgaagcgc gtttacatcg
    18241 tgaaattacg ggaatgctga aaagtatcac gttgagccgc agcgcaaccg ggaaatacta
    18301 cgcgtcgtta ctttgtgacg acgggatgga agcgccggca aagcctgcac tgatatcaaa
    18361 tgtgacaggg ctggatatgg ggctggcgca ttttgtcatc aaatcggacg gcaataaaat
    18421 tgccagtccc cgccacctaa tcaatgcaag tcgcaacctt cgccggaagc agaaagcact
    18481 gtcccgtaag aaaaaaggga acgcaaacag gcgcaaagcc agaatacaac ttgcagccgt
    18541 tcacgaacgg gtagccagtg cccgtgccga tttccagcac aagttatctc gcgcaattgt
    18601 tgacgaaaac caagcggtaa ttgtcgagac attgaaaacg gcaaatatga tgaaaaatca
    18661 ccatctggct cgtgcgatag gcgatgctgg atggtctgga ttcatcacaa aactggaata
    18721 caaagcaaca gagaaaggtg tgcatctggt gaaactggat caatggttcg ccagttcgaa
    18781 aacctgccac tgctgcggtc acaaaatgtc ggagatgcca ttacataagc gcatctggca
    18841 gtgtccagag tgtggagttg aacatgaccg cgatatcaac gcggcgctca acatcaaacg
    18901 caagggcata ctggaattac aagcggcggg actcgtcgtt tctgcccatg gaggccagca
    18961 taaatccgtc acacagacgg ttgcggctta agaagtggga agcctcgccc gacagggcgg
    19021 ggagcagtca catgatgaca ggtaatggcg cggatatcga atgttatgca gacagagaaa
    19081 atcagcctgt tcaaatggct gtgcggttct ggatagcccg aaatagtcaa cttcaggcta
    19141 tcctgagagc ggaattattc cgccaaagtg cgtttttgtt gttcgagatc gcgttcaatg
    19201 ccttcacgaa cgtcctgggg gattttcagc gcgtcaccca gtgcattcag gtaactgcgt
    19261 tccataaaat ggtcaatatc aatagccgcg cagctcagga aatagatttc cagcgcctct
    19321 tcttcattgc ggaccccggt agccaggcgt tgtggatcca gcggttgttc gattgcctgc
    19381 tcaatgagta cacgcccctg ctcttccacg ccggcttcac gcaattgctg gtcgattgcc
    19441 gcacgttctc tggcatcaat atgaccatca cttttagcgg caaagaccag cgcaaggatc
    19501 aaacgttccg tacgctcatc cagcggcgta ctttgcgcgc caaactgcgg ttcgtcctga
    19561 tgcgccgcgc gaattttatc tttgtattta ttccacagca ccgtacccgc gaccgctccg
    19621 ccgccaacca gtaacgcgtt ggtgccatat ttcgtaagaa gtttacgtgc tgatttattt
    19681 gcgaccagca gcccagccag tccgcctaat gcgcctggca ctaaaagttt gcccaatccc
    19741 tgatccgcag acgaggaggc agaagaactg ctttgcccaa gcaaggattg cagctgattt
    19801 aaccagttag ccatatttgc tcctcaataa ccatttttag tgcctgtagc atagcggatg
    19861 aggatgtcag aaaatgtata tcgagacaaa agatgcgcaa ataagcgggc cggcaggccg
    19921 ttattcaaag aaattcgcgc caggtaaggt attcactgta tatcaggtga taagggtatt
    19981 ttaggtgaaa gtaatttgac gttttgagtg gcattgtttg atgctgattg cccgatgcga
    20041 cgctgttgcg tcttctcagg cctacgattg tggtgcgttg tggggcggac aaggcattcg
    20101 cgccgcatcc gccgacggtc aatcgaatta tcagaggcca ttaacgccgc tgatattccg
    20161 ccattcccgc tttgcagtcc acgctgacct ggtaatgaat atcggcactt ttaccacgta
    20221 cggttagcgg tactgaccat ttatcatctt taccctgaat gtcctgcaaa ctgacccatg
    20281 ccacaggatc ggcctgaccg acaatttttt gatcatctgc ccagcgcgcc acgcgatttt
    20341 gttgataatc acgttttacg ctcgcggcaa ttccggcagc atccagacct tcacactttg
    20401 ggaaagtgac cgacttgctg gtttcattat tggcagcgaa aactgatgcg caagcagaaa
    20461 ccaacaacag ccctaaaaac gcccctcttt ttttcatgtt tttctccata gcacaatgat
    20521 tcaggagaaa gcatggtaca aattgtcagg agcgcaagtt gcttcaggcc gcgtgtgagg
    20581 catcttcctc ttcggattca gcaccgacga cctgtgggga cggtaatttg ccggttttca
    20641 gaatggtgct cagcacatct ttttgttctg ccagccatag cgaaagggct tcacgttgct
    20701 cgtcttccat ttgcactgga gaatttgtca gccaggtgtc aagcagatcc gccgtatcaa
    20761 gcattttgtc ataagcatcg gcttcctttt tgctggtaaa cgacattttc tcttcgccct
    20821 cacgaatgac tacgtattta acttcaaccg ccatttgcag cctctcataa taactgtgat
    20881 tttatacagt atatttcttt tcggttggga aatcaacatc agcaataaag acacgcgcaa
    20941 acgttttcgt ttatactgcg cgcggaatta atcaggggat attcgttatg acgttattag
    21001 gcactgcgct gcgtccggca gcaactcgcg tgatgttatt aggctccggt gaactgggta
    21061 aagaagtggc aatcgagtgt cagcgtctcg gcgtagaagt gattgccgtt gatcgctatg
    21121 ccgacgcacc agccatgcat gtcgcgcatc gctcccatgt cattaatatg cttgatggtg
    21181 atgcattacg ccgtgtggtt gaactggaaa aaccacatta tatcgtgccg gagatcgaag
    21241 ctattgccac cgatatgctg atccaacttg aagaggaagg actgaatgtt gtcccctgcg
    21301 cgcgcgcaac gaaattaacg atgaatcgcg agggtatccg tcgcctggcg gcagaagagc
    21361 tgcagctgcc cacttccact tatcgttttg ccgataacga aaaccttttc cgcaaggcgg
    21421 ttgctgacat cggctatccc tgcattgtta aaccggtgat gagttcctcc ggcaaggggc
    21481 agacgtttat tcgttctgca gagcaacttg ctcatgcatg ggagtacgct cagcaaggcg
    21541 gtcgcgccgg agcgggccgc gtaattgttg aaggcgtcgt taagtttgac ttcgaaatta
    21601 ccctgctaac cgtcagcgcg gtggatggcg tccatttctg tgcaccagta ggtcatcgcc
    21661 aggaagatgg cgactaccgt gaatcctggc aaccacagca aatgagccag cttgcccttg
    21721 aacgtgcgca agagattgcc cgtaaagtgg tgctggcact gggcggttat ggtctgtttg
    21781 gtgtcgagct gtttgtctgt ggtgatgagg tgattttcag tgaggtctcc cctcgtccac
    21841 atgataccgg gatggtgacg ttaatttctc aggatctctc agagtttgcc ctgcatgtac
    21901 gtgccttcct cggacttccg gttggcggga tccgtcagta tggtcctgca gcttctgccg
    21961 ttatcctgcc acaactgacc agtcagaatg tcacgtttga taatgtgcag aatgccgtag
    22021 gcgcaggcct acagattcgt ttatttggta agccggaaat tgatggcagc cgtcgtctgg
    22081 gggtggcact ggctactgca gagagtgttg atgaagcgat tgagcgcgca aagcacgccg
    22141 cagcacagat aaaagtacaa gcttaacccc ggcgaaaaac gctacaaaaa tgcccgatcc
    22201 tcgatcgggc attttgactt ttacagctta gcgccttcta cagcttcacg cgccagctta
    22261 gtaatgcggt cgtaatcgcc cgcttccagc gcatctgccg gaaccagcca ggaaccaccg
    22321 atgcacagca cgcttttcag cgccaggtag tcacggtagt tagccggaga aataccaccc
    22381 gtcgggcaga aacggacctg ggagaacgga cccgcgatcg cctgcagggc tttcacaccg
    22441 ccgttagctt cagccgggaa gaatttgaac tctttcaaac cgtagtccat acccagcatc
    22501 agttcggaaa cagtgctgat ccccggaatc agaggaatag tcccttcggt agcagccttc
    22561 agcagcggct cggtcagacc cgggctaatt gcgaactgtg cacccgcttc ggtgacttct
    22621 gccagctgct gtggattcag caccgtaccg gcacccacaa tcgcttcagg cacttctttg
    22681 gcgatagcac ggatagcgtc aactgcacac tcggtacgca gagtcacttc cagaacgcgc
    22741 accccaccag caaccaacgc ttttgccatc ggcaccgcgt gttccagttt ttttaccacg
    22801 ataaccggta caaccgggcc ggtggtcagg attgattctg cacttgtttt ccagtttttc
    22861 atcagagttt tctctcgcct gattacaaat ttgtcgtctt aaaaagtgat acaggttgcg
    22921 ccctgttcgg caccggacag tttttcacgc aaggcgctga ataattcacg tcctgttccc
    22981 acgcgtgacg cgctcaggtc aggaatgtgt ggttcgcgag cagccagttc cgcttcgtct
    23041 accagcagcg tcagttcgcc tgtctgtcca ttcacacgaa tgatgtcccc gtcgcgcact
    23101 tttgccagca gcccgccatc gtaggcttct ggtgttacgt ggatagctga cggcacttta
    23161 cctgaagcgc cggagagtcg tccatcagta actaacgcaa ttttgaaaca ccggtccaat
    23221 aatacaccaa gtggcggcat gagtttatgt aattctggca ttccgttcgc ttttggcccc
    23281 tgatgacgga caacaacgac acaatcgcgg tccagcaaac ccgcttcaaa ggccggcata
    23341 acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa tcacctggtt ctctaccggc
    23401 acggcagagg ttttcataac cgcacggccc aggttaccgc ttaacacttt tgtcccacca
    23461 tgatgagaga aaggttgttc gaaggaagcg atcacattgc tgtcgagtga tttttccgcc
    23521 ccttcccgcc agtccagttc accattattc agccatggtt caagggtata acgagacaga
    23581 ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc ctgctttgag cagttcacgc
    23641 accagaaccg gtacgccacc tgccgcctgg aagtggttaa tatcggccgg accgttcggg
    23701 tagagacgtg ccatcagcgg tacaacatca gaaaggtcag aaaagtcatc ccagttaatc
    23761 tgaataccgg ccgcgcgtgc catcgccacc aggtgcatgg tgtggttagt ggaaccaccg
    23821 gtcgccagca gtgcaacgat accgttcacc accactttct catctatcat cttaccgatc
    23881 ggcatccatt cattaccatt accggtcatg cgtgtaacct ggcgcgcagc ggctgcggtc
    23941 aaagcatcgc gcagcggaga atccggatga acaaaagaag agcctggcaa ctgcatcccc
    24001 ataaactcca ccaccatctg gttggtgttg gcagtaccgt agaaagtaca tgttcccggc
    24061 gcatgataag acgcggcttc tgactccagt aatgccatgc ggtccacttt accttcggca
    24121 taaagctggc gaatacgcac tttttcttta tttggcaaac cgcttgccat cggtccagac
    24181 ggcacaaaca ccgcaggcaa atgcccaaac gacagggctg ccatcgtcag acccgggaca
    24241 atcttgtcgc acacaccgag gaacagagca ccatcaaaca tgttatggga cagccccacc
    24301 gccgcagaca tcgcaatcac ttcgcggctt aacagcgaca attccattcc atcctgcccc
    24361 tgggtgacac catcacacat cgccggaaca ccgcctgcaa cctgaccaac cgcatttgct
    24421 tcatgcaggg ctttacgaat gatttctgga tagtgttcat aaggctggtg cgcggagagc
    24481 atgtcgttat aggaggtgat gatggcgata ttgttacgca acatgctttt caaagaggct
    24541 ttgtcttctg gctggcaggc agcgaaaccg tgtgccaggt taccgcatgc caactgcgaa
    24601 cgatgaacgg tcgaagtttt cgcttgttct atccgggcga gataagcaga gcgagtctcg
    24661 cgcgaacgtt caatgattcg atttgttacg cgtaacaatt gtgggttcat aaaggctcct
    24721 gaaattgagt tgtcagagca ggatgattca caacgcgttt cattcagagg atttatgact
    24781 gaaacgcctg taaccggagc tcatagggca aaaacgtttc agtcagtgta ataaaaaaag
    24841 cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa ttgttctaca atctgtgcaa
    24901 gatcatgtta ccggtaaaat aaccataaag gatgagcgca gatattactc aaactcattc
    24961 caggaacgac catcacgggt aatcatcgcc actgaggcaa caggtcccca ggttccggcc
    25021 tgatacggtt tcggcgcatc attgtccatc gcccacgcct cagtaatgga gtctacccat
    25081 ttccaggctt cttccacttc atcgcgacgt acgaacagtg cctgaatacc acgcatggtt
    25141 tccagcagca gacgttcata ggcatctgcc agatgcgttt gattaaaggt ttctgaatag
    25201 ctcagatcca gcttggtgat ttgcaggtta tgtttatggt caaggccagg aactttattc
    25261 agtacctgga tatccacgcc ttcatcaggt tgcagacgga tagtcagttt attctgcggc
    25321 agatcctgcc acgactcttt aaacagattc agttcaggtg ttttgaaata gaccacgact
    25381 tcagaacatt tggtcggcag acgtttacca gtacgcaggt agaatggcac accggcccag
    25441 cgccagttat caatgtcgac gcggatcgcc acgaaggttt ctgtattgct gctcttgttc
    25501 gcgccctctt cttccagata tcccggcact tttttgccct gggcgaagcc cgcagtatat
    25561 tgcccgcgta cggttttttc gcgcacgttg gagcggtcaa tgcggcgcag agacttcagt
    25621 actttcactt tttcatcgcg gatgctgtct gcactcaggt cagacggcgg agacatcgca
    25681 atcatgcaaa gaatttgcag caggtggttc tggatcatgt cgcgcatctg accggcttta
    25741 tcaaaatagc cccagcgccc ttcgatcccc acttcttctg ccacggtaat ctcaacatga
    25801 tcaatggtgc gattgtccca gttattcaca aacagggagt tagcaaaacg cagcgccaac
    25861 aggttcagca ccgtttcttt accaagatag tggtcgatac ggtaaacctg gcactcctcg
    25921 aagtattcgc caacctgatc attgatttcc tgcgaggtcg ccagcgacgt ccccagcggt
    25981 ttctccatga ctacgcgtgc cggtttagca ttcagttttg cctcaccaag ccctttgcaa
    26041 attgcgccaa aagtgctggg cggcatagca aagtagttaa tggtgatacg atttttttga
    26101 tccagcatcg cgccgagacg gctgaatgca gcagtgtcat tgacatcgag attacaaaaa
    26161 tccagacgtg cactcagggt gtcccataaa ccttcatcaa tggtttcttt catgaaagtt
    26221 tcgagcgcct cgcggacaac tttggtataa gccgctttat cccagtcagc acgccctacg
    26281 ccgataatcc gggtgtccgg gttgagctga ccggcttttt ccagttgata cagggaaggc
    26341 agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa tgaccaggtc acaggcctgg
    26401 gctgtttgcg ttaccgccat gtcattctcc ttaagttaac tatcccggta cttaagccag
    26461 ggtatacttg taattttctt acggtgcact gtactgcttt tacgagcttg cgaaaactgt
    26521 aaacgcttat ccacccgtgc gattacggta aaagcgcgca aagtgcggca aaactgataa
    26581 aaaaatcatc gttttccatt gggtaaaaat ctgacactga tcatgttatg aaaaaaaata
    26641 acaacttttt tatctgcttt tgtcattaac acagcacaca ggcgtaatat ctgacaaaac
    26701 tgcatttcga tttctttcag tgcggaaatc gtcattaccc gtgagtctct ttacatcatg
    26761 aatatgctgg aaaaaatcca gtctcagctg gaacatttga gcaaatcaga gcgcaaagtt
    26821 gccgaggtca ttctggcttc gcccgataac gcgatccatt cgagtattgc tgctctggcg
    26881 ctggaagcca atgttagcga accgacggtg aatcgtttct gtcgcagcat ggacacgcgc
    26941 ggttttcctg attttaaact tcatctggca caaagtctgg cgaatggcac tccctatgtt
    27001 aatcgcaacg tcaatgaaga tgacagcgtt gaatcataca cggggaaaat atttgagtcc
    27061 gcaatggcaa cgctcgatca tgtccgtcat tcactggata aatctgccat caaccgcgcc
    27121 gtcgacttgc tcactcaggc aaaaaaaatc gcctttttcg gattaggctc ttcagccgcc
    27181 gttgcccacg atgcgatgaa taagttcttt cgttttaatg ttccggtggt gtactccgat
    27241 gatattgtgc tgcaacgcat gagttgtatg aattgtagcg acggagacgt ggtggtgctg
    27301 atttctcaca cgggaagaac aaaaaatctg gtcgagctgg cgcagctggc acgcgaaaac
    27361 gacgccatgg tgattgccct cacctctgca ggtaccccgc tcgcccggga agccacgcta
    27421 gcaattaccc tcgacgtacc ggaagatact gacatttata tgcccatggt ttctcgactt
    27481 gcacagctga ccgtgataga tgtgctggcg acaggattta ctttgcgacg cggtgcaaaa
    27541 ttcagagata acttgaagcg ggtcaaggaa gcgctgaagg aatcgcgttt tgataagcag
    27601 ttacttaatt taagtgacga tcgctaaaaa cgactgtcac tgtcctaatc ttatacgaca
    27661 tccgaatgag attaatttat agccatcgcg gcgttatttc attcggattt catgttcaag
    27721 caacacctgg ttgtttcagt caacggagta ttacatgtcc agaaggcttc gcagaacaaa
    27781 aatcgttacc acgttaggcc cagcaacaga tcgcgataat aatcttgaaa aagttatcgc
    27841 agcgggtgcc aacgttgtac gtatgaactt ttctcacggc tcgcctgaag atcacaaaat
    27901 gcgcgcggat aaagttcgtg aaattgccgc aaaactgggg cgtcatgtgg ctattctggg
    27961 tgacctccag gggcccaaaa tccgtgtatc cacctttaaa gaaggcaaag ttttcctcaa
    28021 tatcggggat aaattcctgc tcgacgccaa cctgggtaaa ggtgaaggcg acaaagaaaa
    28081 agtcggtatc gactacaaag gcctgcctgc tgacgttgtg cctggtgaca tcctgctgct
    28141 ggacgatggt cgcgttcagt taaaagtact ggaagttcag ggcatgaaag ttttcaccga
    28201 agtgaccgtc ggtggtcccc tctccaacaa taaaggtatc aacaaacttg gcggcggttt
    28261 gtcggctgaa gcgctgaccg aaaaagacaa agcagacatt aagactgcgg cgttaattgg
    28321 cgtagattac ctggctgtct ccttcccacg ctgcggcgaa gatctgaact atgcccgtcg
    28381 gctggcacgc gatgcaggat gtgatgcgaa aattgttgcc aaggttgaac gtgcggaagc
    28441 cgtatgcagc caggatgcaa tggatgacat catcctcgcc tctgacgtgg taatggttgc
    28501 acgcggcgac cttggtgtgg aaattggcga tccggaactg gtcggcattc agaaagcgtt
    28561 gatccgtcgt gcgcgtcagc ttaaccgcgc agtaatcacg gcgacccaga tgatggagtc
    28621 aatgattact aacccgatgc cgacgcgtgc agaagtcatg gacgtagcaa acgccgttct
    28681 ggatggtact gacgctgtga tgctttctgc agaaactgct gctggtcagt atccttcaga
    28741 aactgttgca gccatggcgc gcgtttgcct gggtgcggaa aaaatcccga gcatcaacgt
    28801 ttctaaacac cgtctggacg tgcagttcga caatgtggaa gaagctattg ccatgtcagc
    28861 aatgtacgcg gctaaccacc tgaaaggcgt tacggcgatc atcaccatga ccgaatcggg
    28921 tcgtacggcg ctgatgacct cccgtatcag ctctggtctg ccaattttcg ccatgtcacg
    28981 ccatgaacgt acgctgaacc tgactgctct ctatcgcggc gttacgccgg tgcactttga
    29041 tagcgctaat gacggcgtag cagctgccag cgaagcggtt aatctgctgc gcgataaagg
    29101 ttacttgatg tctggtgacc tggtgattgt cacccagggc gacgtgatga gtaccgtggg
    29161 ttctactaat accacgcgta ttttaacggt agagtaagta cgttgccgga tgcggcgaaa
    29221 acgcctcatc cggcctacag ttcaatgata gttcaacaga tttcgaatct tctgaagcaa
    29281 acttgaactt atcacacggc ggaagcctct cctcgcgaga ggcttttttt atttgatggg
    29341 ataaagatct ttgcgcttgt acggttggat ttcgcccggt ttgcgagttt tcagcaattt
    29401 taatatccag gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt cttcattcat
    29461 ccgccgcgca atcgtatggt catccgcctc taacagatca tccatcggtg ggcgcacctg
    29521 aatcgtcagg cgatgcgtct tgccatcata aatcggaaac agcggtacaa cgcgcgcacg
    29581 acacactttc atcaaacgac caatcgcggg caacgtcgct ttataggtgg caaagaaatc
    29641 aacaaattcg ctgtgctctg ggccatgatc ctgatcgggt aaataatatc cccagtagcc
    29701 ctgacgtacc gactggatga atggtttaat accatcattt ctcgcatgca gacgaccgcc
    29761 aaaacgacga cgcaccgtgt tccagacata atcaaaaacc gggttgccct gattatggaa
    29821 catcgctgcc attttctgcc cttgcgaggc catcagcatg gcaggaatat cgacggccca
    29881 accgtgcggc accagaaaga tcactttctc gttattacgc cgcatctctt cgatgatctc
    29941 cagcccttgc cagtcaacgc gcggctgaat tttctccggc ccgcgtattg ccaactcagc
    30001 catcattgcc atcgcttgcg gcgcagtggc aaacatctca tcaacaatcg cttcgcgttc
    30061 agcttcacta cgttctggaa agcagagcga cagattgatt aacgcacgac ggcgtgagct
    30121 ttttcccagg cgtccggcaa aacgtcccag ccgtgccaga atgggatcac ggaactttgg
    30181 cggcgttaaa gcgatacccg ccatcgctgc tacgcccagc catgctcccc agtagcgcgg
    30241 gtggcgaaag gatttatcaa actcaggaat gtattcgcta ttattttttt tcgtttccat
    30301 gcttttccag tttcggataa ggcaaaaatc aatctggtga tagtgtagcg gcgcaacttg
    30361 ccccgcacca aataaaaaaa gccggtactg actgcgtacc ggctgcgaat ggatgttaat
    30421 taatcaaacc gtagctgcgg cacaatctct ttggcctgcg ccaggaattc gcgacgatcg
    30481 gagccggtca gcccttcggt acgcggcagt tttgccgtca gcgggtttac ggcctgctgg
    30541 tttatccata cttcatagtg cagatgcggc ccggttgaac gtccggtatt accggaaagc
    30601 gcgatacggt cgccacgttt caccttctgt cccggtttca ccagaatctt gcgcaagtgc
    30661 atataacgcg tggtgtagct gcgaccatga cgaatagcca cataataacc tgctgcgcca
    30721 ctgcgtttgg caaccaccac ttcaccgtca cccactgaaa gcaccggcgt accctgtggc
    30781 atggcgaaat caacacctct gtgtggcgca acacgaccag tcaccggatt agtacgacgc
    30841 gggttaaagt tagatgagat acggaactgt ttcgccgtcg ggaatcgcaa gaatcctttc
    30901 gccagaccag taccgttacg atcgtagaat ttgccatctt cagcgcggat tgcgtaataa
    30961 tctttacctt ctgaacgcaa acgtacgccc agcagctggc tttgctcacg tttaccatca
    31021 agcatttctc gtgacattaa caccgcaaat tcatcgcctt ttttcagttt gcggaaatcc
    31081 atttgccact gcatggcttt aatcactgcg ctcacttcgg cgctggttaa accggcgttt
    31141 ctggcgctgg caacaaagct tcccccgacg gtacctttca gcagattgtt gacccactct
    31201 ccttgctgca tttcgctggt cattttaaaa ccgttagcgg cagtacggtc ataggttcgg
    31261 gtttcacgac gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc ggttaatgtc
    31321 caggagagtt gttgaccgat tttcaggtta cgcaattctt tgtcggcagc agccagttgg
    31381 gtgatatcac ccatatcaat accatactga ttgagaatgc tgcttagcgt atcgccagtg
    31441 gaaacaacat attcatgcac gcccgcttca ccggcgattt tgtcatccag ttcatcctgg
    31501 ggaatggctt catcttcttg tgcagcttga tcaatcggct cactggcttc aggtaagagc
    31561 gaacgaattt cgttctgttc cagctcaatg gttttgacaa ttggcgtggc atcgcggtga
    31621 taaacatagg gccgccagac agcgacggcc agagtaagaa cggtgagcga ccccaacata
    31681 acgcggtgtg gtcgcggtaa attattaaac gccagggcga cagagcgggc tatctgttgc
    31741 acgtaatcac ttcctcatta atctcctttc aggcagctcg catactggtt ggctaattga
    31801 ctcaggaatt ctgaatagct tgttttaccc agtttgatat tcgtccccag gggatccaac
    31861 gttcccatac gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg cctgaactgt
    31921 ggctcagcaa aaacgcaggt tgctttttgc tcaaccaact gtgttcttat ttcatgtaaa
    31981 cgctgcgcgc caggttgaat ctcagggtta acggtaaaat gaccaagcgg tgtcagtccg
    32041 aactgttttt cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt ccccttgagc
    32101 ggcgcgagct cgttaccaac ctgcttttcg gttgaggcta attgtgcctc aaaatccttc
    32161 aggttggcgt caagtttggc tcgactttgc ggcataagtt ccactaattt tccatggatt
    32221 gcaaccgctg tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt gaaatcgccg
    32281 tgatggtgat cttcgtcact tttttccgcg tggtcgtgat catcatcatc gccgtgaata
    32341 cttttcatca gcagcggttt cacattctct agctgcgcaa tcgttacctg tttcgcttca
    32401 ggtaatttac ttaccggttt ttgcatgaac gcttccatct ccgggccaac ccaaacgact
    32461 aagtccgcgt tctgtaagcg ttttacatct gatggacgca gtgaataatc atgttctgaa
    32521 gcgccgtcag gtagtaaaac ctccgtttct gttaccccat cagcaatggc agaagcgatg
    32581 aacccaacgg gtttaagcga agcgacaacg gcagcatctg cggcctgtgt tgcaccgccc
    32641 cagagagcgg cggataatgc tgcgaaaaga agcgtttttt tatgtaacat aatgcgacca
    32701 atcatcgtaa tgaatatgag aagtgtgata ttataacatt tcatgactac tgcaagacta
    32761 aaattaacat gacaagtctg gtttccctgg aaaatgtctc ggtttctttt ggccaacgcc
    32821 gcgtcctctc tgatgtgtcg ctggaactta aacctggaaa aattttgact ttacttgggc
    32881 caaatggcgc aggtaagtcg acactggtac gggtagtgct cgggctggta acacccgatg
    32941 aaggggttat caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag aagctgtatc
    33001 tcgacaccac gttgccactg accgtaaacc gttttttacg cttacgccct gacacacata
    33061 aagaagatat tttgcctgca ctgaaacgtg tccaggccgg gcatctgatt aacgcaccga
    33121 tgcaaaagct ctcgggtggc gaaacacagc gtgtattgtt agcgcgagca ttgttaaatc
    33181 gaccgcaatt attagtgctg gatgaaccca ctcaaggcgt tgatgtgaat ggtcaggtgg
    33241 cgttatatga ccttattgac cagttgcgtc gcgaactggg ttgtggcgtt ttaatggtct
    33301 ctcacgacct gcatctggta atggcaaaaa ccgatgaagt gctttgcctg aatcaccaca
    33361 tttgttgttc cggtacaccg gaagttgttt ccctgcatcc ggagtttatt tctatgtttg
    33421 gtcctcgtgg tgctgaacaa ctgggtatct atcgccatca tcataatcat cgtcacgatt
    33481 tacagggacg aattgttttg cgtcggggaa atgatcgctc atgattgaat tattatttcc
    33541 cggttggtta gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt cgtttgtggt
    33601 ctggcgtcgt atgtcttatt tcggtgatac gctggctcat gcctcattac ttggcgtcgc
    33661 gtttggtttg ttgctggacg tgaatccatt ctatgcggta attgccgtta cgctgctgct
    33721 ggcgggcggt ctggtatggc tggagaagcg cccacagctg gcgatcgaca cattattagg
    33781 gattatggcg cacagtgccc tgtcgctggg cctggtggtc gtgagtctga tgtctaacat
    33841 tcgtgttgat ttgatggctt acctgttcgg tgatttgctg gcagtgacgc cagaagatct
    33901 catctctatt gcaattggcg tggtcatcgt ggtggcgatt ttgttctggc aatggcgcaa
    33961 tttgctgtcg atgacgatta gcccggatct ggcgtttgtt gatggtgtga aattacagcg
    34021 cgtgaaattg ttgttgatgc tggtgacggc attgacgatt ggtgtagcga tgaaatttgt
    34081 cggcgcgttg attattactt cgttgctgat tattcctgca gctacagcgc gtcgctttgc
    34141 ccgcacgccg gaacagatgg ccggtgtcgc tgttttggtg ggaatggtgg cagtaactgg
    34201 cggtttaacc ttttccgcat tttacgatac gccggcgggt ccgtcggtag tgctatgtgc
    34261 ggcactgtta tttattatca gtatgatgaa aaagcaggcc agctaatctg tcgctgaaca
    34321 catttgtcgg atgcggcgcg agcgccttat ccgacccact gttcgctatc tctggtaggc
    34381 ctgataagac gcgaatgaac ggcggcgcat caggcaacac tgccagtgtc ggatgcgccg
    34441 cgagcgaccg atccgactta cggcatttct ggcggcgtga tgccgaagtg gttccacgcc
    34501 cgcactgtcg ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg aatcaaataa
    34561 ggttccagca catcctcaat ggtttcacgt tcttcgccaa tggcagccgc caggttatcc
    34621 aggcctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt gcggtccata
    34681 taatcgaaac cttcggcatc gacattcagc atatccagcg cctgagcagc gatatcagcc
    34741 gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag cagacggttg
    34801 gcaatacgtg gcgtaccgcg agcgcgacga gcaacttcca gcgcgccgtc atcactcatc
    34861 tcaagcccca taaagcgtgc gctgcgactg acgatatatt gcagatccgg cacctgataa
    34921 aactccagac gctgcacaat accaaaacga tcgcgcaacg gtgatgtcag cgaacctgcg
    34981 cgcgtggttg caccaatcag ggtaaacggc ggcaaatcaa ttttaatgga gcgtgccgcc
    35041 ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccggata caacacctct
    35101 tccaccactg gcgaaagacg gtggatctca tcaataaaca gcacgtcatg cggttcaagg
    35161 ttagtgagca tcgctgccag atcgccggcc ttttccagca ccggaccaga agtcgtgcgt
    35221 aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg tagttttacc caaccccgga
    35281 ggaccaaaaa tcaacagatg atcgagggca tcgccgcgca gtttcgctgc tttgatgaaa
    35341 atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa tttagggcga
    35401 atggcgcgat ctgccacatc ttccggcaaa gtggtaccgg cagaaatcag acggtctgct
    35461 tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat gtttcactgc
    35521 tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt ttatagccca
    35581 gcgccaccag cgcggcaacc gcttcctgtt cagcatcgtc ggtcgccggg ctggcaggtg
    35641 atgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct ttaaatcggt
    35701 ctttcatttc aacaatcaag cgttcggcgg tttttttgcc aatacccggc agtttcacta
    35761 gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac attccggaga
    35821 ggatcgccag cgccaacttc gggccgacgc cgttggtttt gatcaactct ttgaacaacg
    35881 tgcgctcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc accacaaagt
    35941 gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag gtcatcggca
    36001 tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt tgtttttcaa
    36061 tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat caaagataat
    36121 gctgtatgat aaaaaaatgc tggatagata tccagcgaag gatgaagaaa acttgcgagg
    36181 cgtcccgatg atctgaaaaa tggcgcagta gaattttttc tacaggttat attggaagca
    36241 aatatttaaa tattacatat tcagcgaaga aatgcgtaat aaaaatacat attgcgcctc
    36301 ctgaaaaaat aaatttttta tgctattacg tatattcgta tctatttcaa tggaatgaca
    36361 atgtgaatat taattatcct gctgaatatg aaattggtga tatcgtcttt acatgtatag
    36421 gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat cacgtcggga
    36481 tcattatcgg tcataacggt gaagacttca tggttgcaga aagccgggtt cccctttcaa
    36541 ccatcaccac gctatctcgt tttattaaac gttcttctaa tcaacgctat gctataaagc
    36601 gattagacgc cgggcttaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc
    36661 ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa
    36721 aatttgtttt tgatatttat aaaaaagcgc tatgtattcc ggtgggtgaa atagagacgt
    36781 ttggagaatt gttaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttct
    36841 taggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg
    36901 gtttggtgtt gattcatgcg gagggagtgg aaacgcctca gccggaactg accgaggcgg
    36961 tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcatcgc
    37021 attctgactg acgtgacaat gggtaatcgc gatagctaac gcatcggcgg cgtccgcctg
    37081 tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcagc
    37141 actaccaata cctaccaccg tttgctttac ctgacgtgcc gcatattcaa ataccggcaa
    37201 ttcctgattc accgccgcca caatcgccac gccgcgtgcc tgccccagtt tcagggctga
    37261 gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct ggaactgggt
    37321 aatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat catccacttt
    37381 ggtgcgtatg catccgctac ccaggtagga caattgcctg cctacctggc ggatgacgcc
    37441 atagccggtc acgcgcgaac ccgggtcaat gccgagaata atagccatca cgcgtctccg
    37501 ttttgctgtt tagcaggccc catcagagag tcgctgcaac ctcatcagag atttcaccgt
    37561 tatggtagac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt
    37621 tcggtgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa acttccgcgc
    37681 tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt tcttcccatg
    37741 cagtgtagac atcaattgcg ccgtcatcat aggtcacaac gtcttcagca cctgcttcca
    37801 gcgctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc
    37861 tgaacagata agctacggaa ccatcagtac cgaggttacc gccacattta ctgaatgcat
    37921 ggcgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg
    37981 tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc
    38041 ccacaccacg tgcgattgcg cggttcagag tgtcacgggt catgttgtta gacaatgctt
    38101 tatcaattgc tgcacgcaga cgcgggttag cgtccggatc gccaccgccg agcttagctg
    38161 cagttaccag ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag
    38221 ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata
    38281 gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag
    38341 gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaggccag atgttcagtg
    38401 aagacgacct ggcgttcgtg cgggagcgca agacagaacc atgattccgt attacgcgtc
    38461 acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaattctac cgtgcgctga
    38521 cagtcaatta aggtcagttg ttcagcgaca acatcgatgg tgacctcttc ctttacttcg
    38581 cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag
    38641 aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcatagatg
    38701 accaccaaga tcgaaacggg acgcttataa gtcatatcag ttattctctg ccttcttcac
    38761 aacctgaatg ctcagctcag ccagtgcagt cgggttagca aagctcggtg cttcagtcat
    38821 cagacacgcc gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc
    38881 ggtcagcagc atggtcaaac ggtcaagacc gaatgccagg cctgcgtgcg gcggagtacc
    38941 gtatttcagg gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc
    39001 cagaatacca aacaccgtct gctgcatatc accattatgg atacgcacag aaccaccgcc
    39061 cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt ccggtgcagc
    39121 tttcagttct gctgccgtca tgtcttttgg tgaggtgaac ggatggtgca ttgccgtcag
    39181 gccgccttca ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt
    39241 gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgcac ccatcgcgtc
    39301 ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt
    39361 acgatccaga atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat
    39421 accttccaga cctttcgcgc gctcgttaac tttaatgtaa gccagacctt tcgcgccgta
    39481 gattttaacg aagttaccgt attcgtcgat ctgtttacgg gtcagcgatg cgccgcccgg
    39541 aacgcgcaga gccgctacgc gacctttcgg atcgttcgcc ggacctgcaa atactgcaaa
    39601 ttcaacggat ttcagcagat cggcaacgtc ggtcagttcc atcgggttac gcagatccgg
    39661 tttatcagag ccgtaacggc gttcggcttc cgcaaaggtc attaccggga aatcgcccag
    39721 atccacgccc ttcacttcca gccacagatg acgcaccagc gcttccatca cttcacgcac
    39781 ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc
    39841 agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtaacggt caaaaccgga
    39901 catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt
    39961 gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat
    40021 cggagtttcg atgtcgagga agccgtggtc atccataaaa cggcgcacca ggctggtgat
    40081 tttagcgcgg gttttcaggc gctgagccat ttccgggcga cgcaggtcga ggtagcggta
    40141 tttcagacgc gcttcttcgg tattgacgtg gttagagtca agcggcagaa catctgcgcg
    40201 gttgatgata gtcagcgagg acgccagcac ttcgatttcg ccggtcgcca tatcgcggtt
    40261 aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg
    40321 cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat
    40381 accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac
    40441 ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc
    40501 tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga
    40561 ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg
    40621 aaattctgcc gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat
    40681 cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa
    40741 attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgagag gcacgatttt
    40801 gttttgtcag gagtcatcat gcttgaactt aatgctaaaa ccactgcgct ggtggtgatt
    40861 gatttacaag aaggcatctt a
//
LOCUS       DAYPHN010000022        40392 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000022 DAYPHN010000000
VERSION     DAYPHN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.022"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1021)
                     /gene="hypF"
                     /locus_tag="ACG0BW_001182"
     CDS             complement(<1..1021)
                     /gene="hypF"
                     /locus_tag="ACG0BW_001182"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFK7258172.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDACNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALAD"
     gene            complement(1174..1701)
                     /gene="hydN"
                     /locus_tag="ACG0BW_001183"
     CDS             complement(1174..1701)
                     /gene="hydN"
                     /locus_tag="ACG0BW_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFK7258173.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(1850..2860)
                     /gene="ascG"
                     /locus_tag="ACG0BW_001184"
     CDS             complement(1850..2860)
                     /gene="ascG"
                     /locus_tag="ACG0BW_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFK7258174.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     IGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SR"
     gene            3120..4577
                     /gene="ascF"
                     /locus_tag="ACG0BW_001185"
     CDS             3120..4577
                     /gene="ascF"
                     /locus_tag="ACG0BW_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFK7258175.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTIAKEVSLN"
     gene            4586..6010
                     /gene="ascB"
                     /locus_tag="ACG0BW_001186"
     CDS             4586..6010
                     /gene="ascB"
                     /locus_tag="ACG0BW_001186"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFK7258176.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFLEKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(6168..6638)
                     /gene="hycI"
                     /locus_tag="ACG0BW_001187"
     CDS             complement(6168..6638)
                     /gene="hycI"
                     /locus_tag="ACG0BW_001187"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFK7258177.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(6631..7041)
                     /gene="hycH"
                     /locus_tag="ACG0BW_001188"
     CDS             complement(6631..7041)
                     /gene="hycH"
                     /locus_tag="ACG0BW_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFK7258178.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAT
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(7038..7805)
                     /gene="hycG"
                     /locus_tag="ACG0BW_001189"
     CDS             complement(7038..7805)
                     /gene="hycG"
                     /locus_tag="ACG0BW_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFK7258179.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(7805..8347)
                     /gene="hycF"
                     /locus_tag="ACG0BW_001190"
     CDS             complement(7805..8347)
                     /gene="hycF"
                     /locus_tag="ACG0BW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFK7258180.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRICNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(8357..10066)
                     /gene="hycE"
                     /locus_tag="ACG0BW_001191"
     CDS             complement(8357..10066)
                     /gene="hycE"
                     /locus_tag="ACG0BW_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFK7258181.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKQEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(10084..11007)
                     /gene="hycD"
                     /locus_tag="ACG0BW_001192"
     CDS             complement(10084..11007)
                     /gene="hycD"
                     /locus_tag="ACG0BW_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFK7258182.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(11010..12836)
                     /gene="hycC"
                     /locus_tag="ACG0BW_001193"
     CDS             complement(11010..12836)
                     /gene="hycC"
                     /locus_tag="ACG0BW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFK7258183.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPTLIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(12833..13444)
                     /gene="hycB"
                     /locus_tag="ACG0BW_001194"
     CDS             complement(12833..13444)
                     /gene="hycB"
                     /locus_tag="ACG0BW_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFK7258184.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(13569..14030)
                     /gene="hycA"
                     /locus_tag="ACG0BW_001195"
     CDS             complement(13569..14030)
                     /gene="hycA"
                     /locus_tag="ACG0BW_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFK7258185.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14242..14592
                     /gene="hypA"
                     /locus_tag="ACG0BW_001196"
     CDS             14242..14592
                     /gene="hypA"
                     /locus_tag="ACG0BW_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFK7258186.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            14596..15468
                     /gene="hypB"
                     /locus_tag="ACG0BW_001197"
     CDS             14596..15468
                     /gene="hypB"
                     /locus_tag="ACG0BW_001197"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFK7258187.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            15459..15731
                     /gene="hypC"
                     /locus_tag="ACG0BW_001198"
     CDS             15459..15731
                     /gene="hypC"
                     /locus_tag="ACG0BW_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFK7258188.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            15731..16852
                     /gene="hypD"
                     /locus_tag="ACG0BW_001199"
     CDS             15731..16852
                     /gene="hypD"
                     /locus_tag="ACG0BW_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFK7258189.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEEPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            16849..17859
                     /gene="hypE"
                     /locus_tag="ACG0BW_001200"
     CDS             16849..17859
                     /gene="hypE"
                     /locus_tag="ACG0BW_001200"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFK7258190.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            17933..20011
                     /gene="flhA"
                     /locus_tag="ACG0BW_001201"
     CDS             17933..20011
                     /gene="flhA"
                     /locus_tag="ACG0BW_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFK7258191.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSLLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            20056..20484
                     /locus_tag="ACG0BW_001202"
     CDS             20056..20484
                     /locus_tag="ACG0BW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFK7258192.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(20523..20867)
                     /gene="ygbA"
                     /locus_tag="ACG0BW_001203"
     CDS             complement(20523..20867)
                     /gene="ygbA"
                     /locus_tag="ACG0BW_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFK7258193.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYDALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(20944..21078)
                     /locus_tag="ACG0BW_001204"
     CDS             complement(20944..21078)
                     /locus_tag="ACG0BW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258194.1"
                     /translation="MIPELIISGEEANGEFLMVIPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            21154..23715
                     /gene="mutS"
                     /locus_tag="ACG0BW_001205"
     CDS             21154..23715
                     /gene="mutS"
                     /locus_tag="ACG0BW_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFK7258195.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            23820..24683
                     /locus_tag="ACG0BW_001206"
     CDS             23820..24683
                     /locus_tag="ACG0BW_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFK7258196.1"
                     /translation="MRITIASIILIISATAIGAEDTPSHFDIPEQVLNNITKESRNKL
                     QDIIAKAQHGDKASQTVMGLIYLNGLSESPSVKDINKAFYWLNLAAKQNYDEAEFYLG
                     QIYHYGYDNTGPNYRIAIYWYEKAAEKGNEQAQINCANIYQFGPQEFQNINKAKYWLQ
                     KAVQKDNPIAHANLGIIGIQENKYAEAIPHLKIAAEKGQRLGQYNYGTLFFNGQGVPK
                     DIRQAKYWFEKAATQNLPIAQITLGQIYAWGLDGNGINKEEALLWLNKAKAQGQFTDK
                     QINAIISKKID"
     gene            24708..25364
                     /gene="pphB"
                     /locus_tag="ACG0BW_001207"
     CDS             24708..25364
                     /gene="pphB"
                     /locus_tag="ACG0BW_001207"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFK7258197.1"
                     /translation="MPSIRYHKIESFNYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPDSLNVLRLLNQPWFTSVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FELNDSEQKEAIDLLLRFHHLPHIIEITNDIIKYVIAHADYPGDEYQFGKEVAEREVL
                     WPVDRVQKSLKGELKEINGADCFIFGHMMFTNIQTFANQVYIDTGSPESGRLSFYKIK
                     "
     gene            complement(25406..25642)
                     /locus_tag="ACG0BW_001208"
     CDS             complement(25406..25642)
                     /locus_tag="ACG0BW_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFK7258198.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVADKR"
     gene            complement(25653..27080)
                     /locus_tag="ACG0BW_001209"
     CDS             complement(25653..27080)
                     /locus_tag="ACG0BW_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFK7258199.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(27080..27673)
                     /locus_tag="ACG0BW_001210"
     CDS             complement(27080..27673)
                     /locus_tag="ACG0BW_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFK7258200.1"
                     /translation="MRLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            27820..28227
                     /locus_tag="ACG0BW_001211"
     CDS             27820..28227
                     /locus_tag="ACG0BW_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFK7258201.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDAEFLGRLSGEEQELFMQLVRKMMSK"
     gene            complement(28348..29340)
                     /gene="rpoS"
                     /locus_tag="ACG0BW_001212"
     CDS             complement(28348..29340)
                     /gene="rpoS"
                     /locus_tag="ACG0BW_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFK7258202.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(29403..30542)
                     /gene="nlpD"
                     /locus_tag="ACG0BW_001213"
     CDS             complement(29403..30542)
                     /gene="nlpD"
                     /locus_tag="ACG0BW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFK7258203.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(30682..31308)
                     /gene="pcm"
                     /locus_tag="ACG0BW_001214"
     CDS             complement(30682..31308)
                     /gene="pcm"
                     /locus_tag="ACG0BW_001214"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFK7258204.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(31302..32063)
                     /gene="surE"
                     /locus_tag="ACG0BW_001215"
     CDS             complement(31302..32063)
                     /gene="surE"
                     /locus_tag="ACG0BW_001215"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFK7258205.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADKVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(32044..33093)
                     /gene="truD"
                     /locus_tag="ACG0BW_001216"
     CDS             complement(32044..33093)
                     /gene="truD"
                     /locus_tag="ACG0BW_001216"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFK7258206.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYVRHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(33090..33569)
                     /gene="ispF"
                     /locus_tag="ACG0BW_001217"
     CDS             complement(33090..33569)
                     /gene="ispF"
                     /locus_tag="ACG0BW_001217"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFK7258207.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(33569..34279)
                     /gene="ispD"
                     /locus_tag="ACG0BW_001218"
     CDS             complement(33569..34279)
                     /gene="ispD"
                     /locus_tag="ACG0BW_001218"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFK7258208.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLTLSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(34298..34609)
                     /gene="ftsB"
                     /locus_tag="ACG0BW_001219"
     CDS             complement(34298..34609)
                     /gene="ftsB"
                     /locus_tag="ACG0BW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFK7258209.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(34803..35126)
                     /gene="ygbE"
                     /locus_tag="ACG0BW_001220"
     CDS             complement(34803..35126)
                     /gene="ygbE"
                     /locus_tag="ACG0BW_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFK7258210.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(35176..35781)
                     /gene="cysC"
                     /locus_tag="ACG0BW_001221"
     CDS             complement(35176..35781)
                     /gene="cysC"
                     /locus_tag="ACG0BW_001221"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFK7258211.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(35781..37208)
                     /gene="cysN"
                     /locus_tag="ACG0BW_001222"
     CDS             complement(35781..37208)
                     /gene="cysN"
                     /locus_tag="ACG0BW_001222"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFK7258212.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVGYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSAGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(37210..38118)
                     /gene="cysD"
                     /locus_tag="ACG0BW_001223"
     CDS             complement(37210..38118)
                     /gene="cysD"
                     /locus_tag="ACG0BW_001223"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFK7258213.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            38370..39407
                     /gene="iap"
                     /locus_tag="ACG0BW_001224"
     CDS             38370..39407
                     /gene="iap"
                     /locus_tag="ACG0BW_001224"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFK7258214.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSSKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   39489..40371
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=39550..39578
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT         9601 a        10945 c        10589 g         9257 t
ORIGIN      
        1 aatccgccag cgcctgttcg ttgctgatag ccggtggttt accgctcagg ttgccggagg
       61 tcatcaccag cggacattgc agttcctgta acagcaaatg ctgaagcggg ttcgcaggca
      121 acattacccc gacttcgtta aggccagggg cgatatcatc acaaagctca ggtacgtatt
      181 ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag caactggcgc gcagcgtctg
      241 gtaaaccttc tgccactggc aacatgaccg ccagcggttt cgccgggcga tgtttgcgcg
      301 cccgaagtgt cgccaccgcg ttactgttac acgcatcgca ggcgagatga aatccgccaa
      361 tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagccgcc tgtaatgccg
      421 cctcttgttc tgcatgttca ccatgactta cccattcaag atgtgggcca cactccgggc
      481 aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc gcgatattct ttgtcacagg
      541 ccggacacag cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc atcgcgcgaa
      601 taatggtgaa acgtgggccg cagtgggtac agttgataaa cggataacga taacgccgct
      661 cgcccggggt attcatttcg gcaagacagg cagggcaagt agcggcatcg ggaacaattt
      721 gcgtattcat ggtgccgcct gtgctctggc gtatagtgaa ctcggtgggc agttgtgacc
      781 agataaacgg ctcacgctcg acgctatcaa tacgcgccag cggcgggcag tgctgatgca
      841 actgcacaag aaacgtttcc gggtcttcca gcagccggac ttccactcca tcgccgtcat
      901 tacagacatc gccgtgaaga tttaattgct gtgccagctg ccagacaaac ggacgaaaac
      961 cgacgccctg cactttgcca cgaatacgca gttggacacc gcaagatgtg ttttttgcca
     1021 ttgagttatt cccgccatca tgaattgcgt aacccgccct gccggacacg acagcgtcgt
     1081 atccggcaat cacaggtcgg cgataccgcc cgctccgtat tctacgaata tttccgggaa
     1141 ttcctttgat gccagaacag ttcagtaaga tttttagaac atcagcgccg tacggcgacg
     1201 tttttctgca ctcagttgtt caagtttatt acgatcgaca caaatcagcg catgggtcgg
     1261 gcaagccgcc atacacgccg ggccgtcttc acgatggtta cagaggtcgc atttattggc
     1321 ttcggctttg tcagcccgta cattcagccc cgcgccgctg ttgcgaatca ccggacgtac
     1381 caccacttcc atcgcgccat acgggcaagc cacaacgcag gttttgcaac caatgcaacg
     1441 ttcctgcatc acatgaacaa accctttatc acggctgata gcaccattcg ggcagacgtt
     1501 agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt tcacaccttt
     1561 aatgacatgg atacgcggta aaaatgtttc cggggtcagc gatgcacagt cctgattttc
     1621 ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc
     1681 agcaatgatg aaacggttca tcaaattctc cagcaatgac agttaatgcg ccgatacatt
     1741 cacaaatcat gccagttttt aatacactgt tatttaagga aattaatttc tgtaatgcag
     1801 gaaaaacgat gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa
     1861 gagcaatgag ggagccgcgg cggatcagtt ttccgctgaa ggttttcggc ggtgagaaat
     1921 ccccgccatc gagcataaaa atcagccgtc caataatttc ctgaatcatc tcagtcaccg
     1981 gaatttttac gctggagagc gccggaacgg tgtagggggc aattgcgata tcatcgaatc
     2041 cgataactga cacctgctct ggcaccgcta cgccgcgctc gtgcaacgct ttcatcgcac
     2101 ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa tttagcccca cgttcgagca
     2161 acgtttctac cccttcggcc ccgctggcgg gcgtccattt accgatagcg ataagttttt
     2221 cattgagcgc aataccatgc tgcgacagcg cgtctttata cccggcaaga cgttcaatgc
     2281 tggtggggga atccatcgag ccggtaagga aagcaatctc ctgatgtccg gcgtttatca
     2341 actctgccac ggcgttaaag ctggtctgtt tatgatcgca ccagacgcta tggctgctat
     2401 ttttgcgcag gcggcgatta agcaccatta tcggctgact gtgtgcgtca atgatgtcat
     2461 caatctcatc cacgcttaaa aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca
     2521 gcagatactg aatcgcctgg cgctcttctt cggcgctgtg tttaccatct gccaatagca
     2581 actgtcgccc tttctcttcc gccattcgcg cggcatgaaa gagtaattca ctaaaataaa
     2641 tgccgtggta aagtgtgttg gtcactacca gccccagcgt ctgagtactc ttcgccgaca
     2701 gattgcgcgc cagcaagttt ggacggtaac cgctctcttc taccgcctga aacacgcgat
     2761 ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg gtcgcttttg
     2821 aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc cctttacacg
     2881 caattaacgc agtgtactgc accgtttgcc gattgtcctt gcgcaatcgg cgggaaaaat
     2941 attcaggtga ccggtttcac aaatataaaa aatgaacaat taactctctt gcttatttag
     3001 tgacaactat tcatgatttt gtgaaaccgg tttcttaatt ccgtttcagc atcggcattt
     3061 ttccgtcacg tcgactgata acaactacat ctaccctact gataacagga taaaatccga
     3121 tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc ggcactgggc ggcgttgata
     3181 acatctcggc agtcacgcac tgtatgacgc gtttgcgctt tgttatcaaa gatgaccagc
     3241 ttatcgacag tccgacgttg aaaaccatct ccggcgtgct cggcgtggta cgtagtgaca
     3301 accagtgtca ggtgattatc ggcaataccg tttcacaagc ctttcaggaa gtcgtcagcc
     3361 tgctgccggg agatatgcag cccgcacagc ccgtgggtaa acccaaactt acgctacgtc
     3421 gcattggtgc ggggatcctc gatgcgctga tcggtaccat gtcaccgctg atcccggcga
     3481 ttatcggcgg atcgatggtc aaactgctgg cgatgatcct cgagatgagc ggcgtgctga
     3541 caaaaggatc gccgacttta accattctga atgtgattgg tgacggtgct ttcttcttcc
     3601 tgccgctaat ggtcgcggca tctgccgcca tcaaatttaa aaccaatatg tcgctggcga
     3661 ttgccatcgc gggtgtgctg gtacatccga gttttattga attaatggcg aaagcggccc
     3721 agggtgaaca tgtagaattt gccctgatcc cggtcaccgc ggtgaaatac acctacacgg
     3781 tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg gtggatcgca
     3841 ttacaccggc ggtgactaaa aacttcctca agccaatgct gattgtgttg attgccgcac
     3901 cgctggcaat tctgctgatt ggcccgattg gtatctggat cggtagcgcc atttcggcgc
     3961 tggtttacac cattcatggt tatctgggct ggctttcagt cgccattatg ggcgcgctgt
     4021 ggcctctgct ggtaatgacc gggatgcacc gcgtctttac gccaaccatc attcagacca
     4081 ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga gatcggcgct aacctgtcgc
     4141 tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa cccggaactg cgccagacgg
     4201 cgctggctgc ggcggcatca gccattatgg cggggatttc cgaaccggcg ttatatggcg
     4261 tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt atttgcggcg
     4321 cggttgccgg tatggcgggg cttgccagcc actcaatggc agcgccgggg ctatttacca
     4381 gcgtgcagtt cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc gcggtcatgg
     4441 cgctggcggt cgtgctgtcg tttatcctca cactgttgct cggctttgag gatattcctg
     4501 ttgaggaagc ggctgcccag gcgcgaaagc atcagagcgt acaaccgacc atcgccaaag
     4561 aagtaagtct taattgagga tgaaaatgtc agtatttcca gaaggttttt tatggggcgg
     4621 cgcgcttgcc gccaaccagt ctgaaggtgc gttccgcgaa ggcggcaaag gactgaccac
     4681 ggtcgatatg atcccgcacg gcgagcatcg aatggcggtg aaactggggc tggaaaaacg
     4741 ttttcagttg cgcgatgacg agttttatcc cagccatgag gcgacggatt tttatcatcg
     4801 ttataaagaa gatatcgccc tgatggcaga gatgggattc aaagtgttcc gtacctcaat
     4861 tgcctggagc cgcctcttcc cgcagggcga tgaactgacg cccaaccagc agggcattgc
     4921 tttttaccgt tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc tggtcacgtt
     4981 gtgccacttc gatgtgccga tgcatctggt caccgaatat ggctcctggc gtaaccgcaa
     5041 gctggtggag tttttcagcc gctacgccag aacctgcttt gaagcatttg atggtctggt
     5101 gaaatactgg ctaaccttca atgaaatcaa cattatgttg catagcccgt tctccggcgc
     5161 gggtctggtg tttgaagaag gggaaaatca ggatcaggtg aaatatcagg ctgcgcatca
     5221 ccagctggtt gccagtgcgt tagccaccaa aatcgcccat gaggttaacc cgcaaaatca
     5281 ggtgggctgt atgttggcgg gcggtaactt ctacccttac agttgcaagc cggaagatgt
     5341 ctgggcggcg ctggagaaag accgggaaaa cctgtttttt atcgatgtgc aggcgcgggg
     5401 cgcgtatccg gcttactctg cccgcgtatt cctcgaaaaa ggggtaacca tcgacaaagc
     5461 accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt gtctccttca gctattacgc
     5521 ctcgcgctgc gcctcggcgg agatgaacgc caacaacagc agtgcggcga acgtggtgaa
     5581 atcgctgcgt aacccatatc tacaggtaag cgactggggc tggggtattg atccactcgg
     5641 tctgcgtatc accatgaata tgatgtacga ccgttatcag aagccgctgt ttctggtgga
     5701 aaacggcctc ggcgcaaaag atgaatttgc tgccaatggt gagattaacg acgactatcg
     5761 catcagctat ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag acggcattcc
     5821 gctgatgggc tacaccacct ggggctgtat tgatttagtt tccgcctcaa cgggtgaaat
     5881 gagcaaacgc tacggttttg tctacgtaga ccgtgacgac gcaggcaacg gtacgctggc
     5941 gcgcacgcgt aagaaatcat tctggtggta taaaaaagtg attgccagta acggtgaaga
     6001 tttagagtaa gtaacagtgc cggatgcggc gtgaacgcct tatccggcct acatgtcgca
     6061 cgaaccaggt aggcccgata agcgcagcgc atcgggcaat ctggcgtttg ctatcagttc
     6121 tgattccctc atcaagaaca tccctgtcct gattccttac tgaaaaacta ctcttcttcc
     6181 accgctaact gtgcgaagcc gccattccct tcccagcctt ccagccgttg ataaacggtt
     6241 tctacggcgt ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat atccggctgg
     6301 atgccgagga aaatcacttc gccaatatct tcttttaact ggtcgataag gtagttcagc
     6361 ggcatgttat gggtagtcat cataaacatc tcggcgatgt catccgggtc gatgatgcgg
     6421 atctcgccgg ggtttagccc catatccgtg gcgtcgacaa tcagcagtcg tgtcgggcgc
     6481 agttcgcgga tagcgacaat gtcgttttcc ggtgcgctac cgccgtcaat caccacccag
     6541 ttacctttcg gcgcggcggc gcacttttcc gccagcagcg ggcctgcgcc atcatcgccc
     6601 atcatgctat tgccaacaca gagtaaaacg tcagtcacgc aatctcctca ccatcaaata
     6661 gatggcgttt tcctgatgaa tatcatgcag catacttaac agcattgtac tccattcttt
     6721 ctgctgcgaa gtttgcgttg cccgggcttt atcgaatgcg ttggccagca tcggcacatg
     6781 gttaatgtcg atgacgatct caccatattt cggcacgcct tccattttgc ggcgggcttc
     6841 gctgcccgcc tccagagtgg caatccatgc cagatattca tcccacgggc aggtgagcgc
     6901 cgcttccagg caatcgataa ccccaaggtg gtgaccaatc gccaggctgt aatagaccac
     6961 ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata aatttacggc tcagttgact
     7021 gaacaccacc ttttcactca tcggatacgc gcctcttcaa caacatgatt cagatggctg
     7081 acaatctcgt tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac ctgctcttcg
     7141 ccctgcccta actgtgtcag gaagtcatcg gcaatctggc gaccgtaacg gtaacccgcc
     7201 aggcggcgtg cttcgcgatc tactttcaca cgcagcggct gcaccatatc gccatgcagg
     7261 atctccgccg gttgttcatc ctgctcaccc ggcccacggg cgtgaatttt ctgctccagc
     7321 aggccgagcg ccattgcaaa gccgtatagc gtagcggcag gcgttggcgg gcagcccgga
     7381 atgtagacat ccaccgggac gattttatcc gtaccgcccc acacgcagta gagatcgtgg
     7441 aaaatgccac cgctgttacc gcaggcaccg taggagatac agattttcgg gtccggcgcg
     7501 gactgccacg cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt
     7561 aaaatatccg catgacgcgg tgaaggaacg actttaatgc cgaagcgttc tgcatcgaac
     7621 agcggcgaca aagtggcgaa aatttcgatt tcgcagccgt tgcagccgcc gcagtccacg
     7681 cggtaaacgt aggcagaacg tttgattttt ttcagtaacg acgccttcat gctggcgatg
     7741 gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc taataaattg
     7801 ctcatcagat ggcctctttc atatggcgag tcagttcaat acggtcggac ggcaccaggc
     7861 atttctggcg cttacattcc gggcaagtct caaagctttc gcggtggttt tccgcgcgac
     7921 tgtcaccatt gtgcttaagc agtgcaatgg cgtagtcgat ctctttctgg acggcgaaag
     7981 gacgattaca gatgcggcag ttacacagcg cgaaacggga ctgttgcagg aagtcttctt
     8041 tcttccacac cgccagttcg tactcttgcg acagtttgat cgccgccgtc gggcagactt
     8101 cttcgcagcg tccacagaag atgcagcgtc caagattgaa ctcccaggca agctctcctg
     8161 tggcgaggtc ggtttcaacc gttaaggcgt ttgacgggca ggcattgacg caggccgcgc
     8221 agccgatgca ctgctgcggg ttctgctctg gcttaccacg gaagttttta tcaaccgcaa
     8281 tcggctccag cggataagac gaggtcgccg tgccggtttt gatgactttt ttgataaagg
     8341 taaacatggc gattccttat ttcagcggcg agtttttacg ctcaatgctg taacgctcga
     8401 gttctttgta cggcaccact ttgctcttct tcttacgtac atcgaccacg gtcatgcggt
     8461 cggtacagga gtagcaaggg tccaggctac cgataatcag cggcgcatcg gaaacagtgt
     8521 tgccacgcag catgtagcgc agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc
     8581 agcggtacag cttctggttg tcgccggtca tgctccagtg gatatcgtcg ccgcgcggcg
     8641 cttcggcaaa gcccagcgca aaacggtgcg gaatataggt aaagccttcc accatcagcg
     8701 gaccgcccgg caggttatcc agaccgtagt cgatcatgtt cagcgcggta tagacttcgt
     8761 tgatacgaac tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg acttccattg
     8821 gcagcaggcc ataaccaaca aacgggtgat cggcgcgggt atcgcgagcg tgtccgctgg
     8881 cgcggaccat cgggccaacg ttactgaagt cgcgggcaat ttccgggtcc agacgaccaa
     8941 tgccgacggt gcgctgttcc atgttcggtg tgctcagcag cacatccacc agctcctgca
     9001 cttcacgacg catctgttgt gccagctggc gggtctggat catgtcgtct ttcagcagat
     9061 cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt acgtgcaccg gtaaggatct
     9121 ctgccatttt catggaggtt tcacgcacgc ggaagaactg cataaagccg gagtcgaagc
     9181 cggtaaagtg acaggcgagg ccgaggttga gcagatgcga gtgcaggcgt tctacctcca
     9241 gcagaatggc gcggatcatc tgcgcacgtt ctggtacctg aatacccatc gcgttttcca
     9301 ccgacgtggt gtaggcggtg ctgtgagcaa agccgcagat cccgcacaca cggtcagaga
     9361 ggaaggtcac ttcgttgtaa cccatgcggg tttccgccag tttttccatg ccgcgatgga
     9421 catagaacag gcggtagtcg gcgtcgataa tgttttcgcc atcgacgaac agacggaagt
     9481 ggcccggttc gtcagaagtg acgtgcagcg gaccaatcgg aacgacgttg tttttcttgt
     9541 cgcccagttc gttgatgaac tcgtaggttt cagcatcggt agtcggtgcc ggacgttgac
     9601 gataatccat gctgtcttta cgcagcggat aaagttcatc cggccagtca tccggcagca
     9661 ccagacgacg ttcatccggc agccctaccg gaatcaaacc gtacatatcg cgcacttcac
     9721 gctcgcccca taccgccgcc ggaacgcgcg gcgttacgga cggatattcc tgtttgttgg
     9781 cgtcaacttc aacgcgcacg gttatccagc atttggtgcc cttctccatc gacagcacgt
     9841 aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt accaaacagc accgacagcc
     9901 agcccccctg cttgtaatag agaaactcca ctacttccgg cagatagttc acctttacgg
     9961 tgacagtcag ctgatctttg gtctgccagg cgtggtccag cacgacgccc ggaaatgcct
    10021 cattcagcgc ggcgagataa tgttgaccta atttttcttc agacatgctc aaactctctt
    10081 taatcacgcc gccagcaagg agacgaacgc taaaaatgca aagccaaacc cagcccaggt
    10141 aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg ttttcgaaca gcgcgataac
    10201 caggacgcca accaccagtt tgacgatggc aatcaccagc gccagcagca acccaccgac
    10261 ggtgaaggtt tccatttgtc cccacggaat aaacaccccg acgaacatct gcaacaccac
    10321 cagctgtttc aggctgatac cccatttcat gacgccaaag ccgctgccgc tatactcaga
    10381 gagcggacct tcctgcaact cctgctcggc ttccgccagg tcgaacggca gtttgcccat
    10441 ttcgataaag gtggcgaatg cacaggcaca aagcgccagc accagcggga tgctctggct
    10501 cagcggccag tgataaacgg tgtcggtgat gttgctgatg ttggtggaac cggcaacctg
    10561 cgccgcaacc cacagaccaa gcagcagcat cggttcgacc agcacgccaa gcatcgcttc
    10621 acggctcgcg ccgatagcgg taaacgggct accggtatcc agaccagaaa tggcaaagaa
    10681 gaaacgcgcg atggcaaaga gatacagtaa ggtgatcaaa tcgcccagct gcggcagcgg
    10741 agaaccaacg gtcaccaccg gcagcgcagt agcgatagtc agcatgacgc ccaccatcac
    10801 atacggcgtc aggcggaaca cccagccgga ggcatccggg ccaacgctct ggcggcccag
    10861 cagtttgata atgtcgcgat actcctgcaa cacgcccggc ccgcgacggt tatgcaagcg
    10921 ggcgcgcgcc acgcgggtta taccggagag cagcggcgca acggcaaata acaccagcgc
    10981 ctgaattaac ggatataaaa cactcattct caggctcctc gtgaaacaat aatcaccacc
    11041 agtaccgcca gttcaaccag cgccatccgg cggaacagca acgcactccc ctcgcactgc
    11101 cagcccggta ccagcgacac cggattcagc catttgcgca gtttcagcac cggcgcaaac
    11161 gcctgtttca ccggcatggc aaaaccgtga gcagtaatca ccattgattt ttcatggtcg
    11221 tagccgcaca cccaggccgc gccgcgggaa cgcgacggca aacgatcgcc tttgcaaatc
    11281 gccataatga tgaatggcag cagcgggcag gcaatcagca gcaacgtgat cattggttga
    11341 gaaacggtgg tgttagcagg ctccagcggc agaggtacag cagcagagag catcggcagc
    11401 agccacggcg cagcaacacc gccaattacg cagcaaatcg ccagtgccac tacgcttacg
    11461 ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt tggtacgcgg cgcgccgagg
    11521 aacgtgacgc catagacttt cgccatacac atcaccgcca gcgcaccggt aattgccaga
    11581 cccactgcga gcagcggccc cagcagacgg gcaacaaacg cgccactatt gctcagtttg
    11641 aagaaggatt gatagataac ccattccccg gcaaacccgt tcagcggcgg cagcgccgcc
    11701 attgccatca gcccgactaa catggcgatg gagataaccg gcattttctt gccaatgcca
    11761 ccgagttttt cgatatcgcg atgaccggta cggaaccaga cgctccccgc cccgaggaac
    11821 agtacgcttt tgaacaggct atggttaagc agatggtaca gaccaccgac cagaccaagg
    11881 gcaatcagcg tcggttgttc gagcgcgata cccgttacgc cagcgcccag ccccagcagg
    11941 ataatgccga tattctccag ggtgtggtaa gccagcaaac gttggatatt atgttccatc
    12001 aacgcataca gaccgccgac aaacgcggtg atcataccga gcaccagcag cgcgatcccc
    12061 caccacagcg gtgcattacc gcccagcagt gacagggtta aaatgcccag caggccaatt
    12121 ttcatgacta ccgtggaaaa caacgcggca gctggcgcag aggcgttagc atgtgcctgc
    12181 ggcacccagc cgtgcagcgg aataatcccg gccagcaggc caaaaccaat cacaccgagc
    12241 agccagatat cggaaccgag cggcagctgt tgcatacgca tatccagcag gcgcagatcc
    12301 agcgtgccgt aacgctgcca cagcagccaa caggcaatcg ccagcagcag agtgccaaga
    12361 cgccccagcg caaaccacag tttgccctct ttgctgttgc tggtaaggaa caccgcgcac
    12421 agggccatga tttcggccat taccacgaac atgccgaggt tgctggcaat gacggcgcag
    12481 acggcggcag ccatcaacat attgatctgc aagccgttgc acttcacctg cgcgtggcga
    12541 tgccagtcaa tgttatagag gctgacaaac agaccgcaca gaccgagcgt aatcagccaa
    12601 atcgcgttaa gcggagagat ttgcacatcg tagcttacca gcgatagcgc accgctcacg
    12661 ccaaccgcgc cagtcagtac agtgaagccc gcggctgccg tatacagact acccaccgcg
    12721 ccgccaattc cggctatcca gccgcttagc gccttttgaa aagaaaagag aaatgccaga
    12781 acagcggcgg cgacaaacca cgccacgccg ctattgatca gggaaattgc gctcatttag
    12841 cctctccact ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg ttaaaggtca
    12901 gctcacgctt acgtttgcta acgcgggcga tatcggtgtt atccaccaga tgcagggctt
    12961 tagtagggca catccgcacg caggccggac cttgttcatc aaaactacaa aggtcacatt
    13021 tgacggcgat cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca cgcgccggag
    13081 caggcggtgc cggtggcgct ttcggggtat tggcgtttgc cggaatatcc agcggacggc
    13141 tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc gcacagtttg cagcttacgc
    13201 acagactttc attcaactgc acggccccat caacgcgggt gatggcgtta accgggcaga
    13261 ccaccgcaca aggcgcatct tcacagtggt gacagagctg cggcgcagat tctttttcat
    13321 tgagcatcac tcgcaggcgc ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac
    13381 aggcggcctc acaggtgtgg cagccgatac agagcgtgga gtcagcaatt acaaaacgat
    13441 tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa acatgccgat gaaatgtcat
    13501 tttcgacact catcgacacg cccatcccca aacaggcgta acgcctgcaa aacgggcaaa
    13561 gcctcagctc atgctgccgg gctttgtccc tttaccagtt ggcttaaatt caccggcaca
    13621 ttgttttcaa tggcggtgta taagctgtcg taaacgccag cgattttttc gagatagtgt
    13681 tccagaacct gttcgcgatc gcggatgttg accttgccat caatcatgcc gtcaatgctc
    13741 agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg
    13801 atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa tgcgaaaatg ttcaaaaagg
    13861 acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat
    13921 aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc actggtcctg taggcgacga
    13981 tgccgctgtg cgatgtaatc ggctttctcg cttatttccc aaatagtcat tgtcaggtta
    14041 cccgtttaac agagatgcca gccttaagca ttttctgtgc caacttttaa ttcattgtta
    14101 tgaaacagat ttttaagata aagatgaatt tcgtcgccgt gtcgacgtgt catttcgaca
    14161 tcatcgacat tattcaccgc agggataatc aacactggca caattattgc ttatggctgg
    14221 taatagttaa tgggaggcga tatgcacgaa ataaccctct gccaacgggc actggaattg
    14281 atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt
    14341 ggcgcatttt cttgtgtcga aaccagctct cttgcctttt gttttgatct ggtttgccgc
    14401 ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac aagaggccga atgctggtgt
    14461 gaaacatgcc aacagtatgt gacgctactg acccagcgcg tccgccgctg tccacagtgt
    14521 catggtgaca tgctacagat tgtggcagac gacggtttac agattcggcg gatagaaata
    14581 gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct gtatatcgag
    14641 ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc
    14701 ccgaagatga aaatcaccgg cattaaaacg cctgaattta cccccagcca gactgaagaa
    14761 ggcgacctgc attacggtca tggcgaagcg ggcactcacg cgccgggcat gagccagcgc
    14821 cggatgctgg aagttgaaat tgacgtgctg gacaaaaaca accgtctggc tgaacgcaac
    14881 cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc tggtttccag ccctggttcc
    14941 ggtaaaacca ccctgctgac ggaaacctta atgcgcctga aagacagcgt tccgtgcgcg
    15001 gttattgaag gcgaccagca aaccgtgaac gatgccgcac gcattcgcgc taccggcaca
    15061 ccagcgattc aggtgaacac cggtaaaggc tgccatcttg acgcacagat gattgccgac
    15121 gccgcgccgc gcctgccact ggacgataac ggtattctgt ttatcgaaaa cgttggcaac
    15181 cttgtatgcc cggcgagctt cgatctcggt gaaaaacaca aagtggcggt gctttccgtt
    15241 accgaaggtg aagacaaacc gctgaaatat ccccatatgt ttgccgccgc ctcgctgatg
    15301 ctgctcaaca aagttgacct gctgccgtat ctcaactttg acgttgagaa gtgcatcgcc
    15361 tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta tttccgccac cagcggcgaa
    15421 gggatggacc agtggctgaa ctggctggag acacagcgat gtgcataggc gttcccggcc
    15481 agatccgcac cattgacggc aaccaggcga aagtcgacgt ctgcggcatt cagcgcgacg
    15541 tcgatttaac gttagtcggc agctgcgatg aaaacggtca gccacgcgtg ggccagtggg
    15601 tactggttca cgttggcttt gccatgagcg taattaatga agccgaagca cgcgacactc
    15661 tcgacgcctt acaaaacatg tttgacgttg agccggatgt cggtgcgctg ttgtatggcg
    15721 aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat
    15781 tgagcatctg cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc ctctgcggat
    15841 tatggaggtg tgtggcggtc atacccacgc catttttaaa ttcggcctcg accagttgct
    15901 gccggaaaac gttgagttta tccacggtcc cggttgcccg gtgtgcgtac tgccgatggg
    15961 cagaatcgac acctgcgtgg agattgccag ccatccggaa gtcatcttct gtacctttgg
    16021 cgacgccatg cgcgtaccag ggaaacaggg ttcgctgtta caggcaaagg cacgcggtgc
    16081 cgatgtgcgc atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac
    16141 ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccacc atgccgacga cggccatcac
    16201 tctgcaacag gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc agcatattac
    16261 gcttatcccg acgctgcgca gtttgctgga agagccggat aacggtatcg acgcgtttct
    16321 tgcgcccggt cacgttagta tggttatcgg cactgatgcc tataatttta tcgccagcga
    16381 ttttcatcgt ccgctggtgg tcgctggttt cgaacccctt gatctactgc aaggcgtggt
    16441 gatgctggtg gagcagaaaa tagcggccca cagcaaggta gagaatcagt atcgtcgggt
    16501 ggtgccggat gccggtaacc tgctggcgca acaggcgatt gccgatgtgt tctgtgtcaa
    16561 cggcgacagc gaatggcgcg gcttaggcgt gattgaatct tctggtgtac acctgacgcc
    16621 ggattatcaa cgattcgatg ccgaagcgca tttccgcccg gcaccgcagc aggtctgcga
    16681 tgacccgcgc gcacgttgtg gcgaagtatt aacgggcaaa tgtaagccgc atcaatgccc
    16741 gctgtttggt aacacctgta atcctcaaac cgcgtttggt gcgctgatgg tttcctccga
    16801 aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt gaataatatc
    16861 caactcgccc acggtagcgg cggccaggcg atgcagcaat taatcaacag cctgtttatg
    16921 gaagcctttg ccaacccgtg gctggcagag caggaagatc aggcacgtct tgatctggcg
    16981 cagctggtag cggaaggcga ccgtctggcg ttctccaccg acagttacgt cattgacccg
    17041 ctgttctttc ctggcggtaa tatcggcaag ctggcgattt gcggcaccgc gaatgacgtt
    17101 gcggtcagtg gcgctattcc gcgctatctc tcctgtggct ttatcctcga agaaggattg
    17161 ccgatggaga cactgaaagc cgtagtgacc agcatggcag aaaccgcccg cgcggcaggc
    17221 attgccatcg tcactggcga tactaaagtg gtgcagcgcg gcgcggcaga taaactgttt
    17281 atcaacaccg ctggcatggg cgcaattccg gcgaatattc actggggcgc acagacgcta
    17341 accgcaggcg atgtattgct ggttagcggt acactcggcg accatggggc gactatcctt
    17401 aacctgcgtg agcagctggg gctggatggc gaactggtca gcgactgtgc ggtactaacg
    17461 ccgcttattc agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt
    17521 ggtggtgtaa acgcggtggt acatgagttc gcggcagcct gcggttgtgg tattgaaatt
    17581 tcagaagcgg cactgccggt taaaccagcc gtgcgcggcg tttgcgaact gctgggactg
    17641 gacgccctta actttgccaa cgaaggcaaa ctggtgatcg ccgttgaacg caacgcggca
    17701 gagcaagtgc tggcagcgtt acattcccat ccactgggga aagacgcggc gctgattggt
    17761 gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa acgaaccctc
    17821 gatttaccac acgccgaacc gttaccgcgt atatgctaat aaaattctaa atctcctata
    17881 gttagtcaat gaccttttgc accgctttgc ggtgctttcc tggaagaaca aaatgtcata
    17941 tacaccgatg agtgatctcg gacaacaagg tttgttcgac atcactcgga cactattgca
    18001 gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcta ctggtaaagc gttctgcgct
    18061 cgccgacaac gcggctattg tgttgtggca agcgcagact caacgtgcgt cttattacgc
    18121 gtcgcgtgaa aaagacaccc ccattaaata tgaagacgaa actgttctgg cacacggtcc
    18181 ggtacgcagc attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga
    18241 aacctggccg cagctggccg caggtgggct atacccaaaa tttggtcact attgcctgat
    18301 gccactggcg gcagaagggc atatttttgg tggctgtgaa tttattcgtt atgacgatcg
    18361 cccctggagc gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt
    18421 caccgaacaa atccagagtc gagtcgttaa caatgttgac tatgagttgt tatgccggga
    18481 acgcgataac ttccgcatcc tggtcgccat caccaacgcg gtgctttccc gcctggatat
    18541 ggacgaactg gtcagcgaag tcgccaaaga aatccattac tatttcgaca ttgacgatat
    18601 cagcattgtc ttacgcagcc accgtaaaaa caaactcaac atctactcca ctcactatct
    18661 tgataaacaa catcccgccc acgaacagag cgaagtcgat gaagccggaa ccctcaccga
    18721 acgcgtgttc aaaagcaaag agatgctgct gattaatctc cacgagcggg acgatttagc
    18781 cccctatgaa cgcatgttgt tcgacacctg gggcaaccag attcaaacct tgtgcctgtt
    18841 accgctgatg tctggcgata ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa
    18901 agtgtttacc actaccaatt tgaatttact gcgccagatt gccgaacgtg tggcaatcgc
    18961 tgtcgataac gccctcgcct atcaggaaat ccatcgtctg aaagaacggc tggttgatga
    19021 aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt gaatttggcg agattattgg
    19081 ccgcagcgaa gccatgtaca gcgtacttaa acaagttgaa atggtggcgc aaagtgacag
    19141 tactgttctg atcctcggtg aaactggtac aggtaaagag ctgattgccc gcgcgatcca
    19201 taatctcagt gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc
    19261 cggattgctg gagagcgatc tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc
    19321 ccagcgtatc ggtcgttttg aactggcgga taaaagctcc ctgttcctcg acgaagtggg
    19381 cgatatgcca ctggagttac agccgaagtt gctgcgtgta ttacaggaac aggagtttga
    19441 acgtctcggc agcaacaaaa tcattcagac ggacgtgcgt ctaatcgccg caactaaccg
    19501 cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc gatctctatt accgcctgaa
    19561 cgtattcccg atccacctgc cgccactacg cgagcgtccg gaagatattc cgctgctggc
    19621 gaaagccttt accttcaaaa ttgcccgtcg tctggggcgt aatatcgaca gcattcctgc
    19681 cgagacgctg cgcaccttga gcaacatgga gtggccgggt aacgtacgcg aactggaaaa
    19741 cgtcattgag cgcgcagtat tgctgacacg cggcaacgtg ctgcagctgt cattgccaga
    19801 tattgtttta ccggaaccag aaacgccgcc tgccgcaaca gttgtcgctc aggagggcga
    19861 agatgaatat cagttgattg ttcgcgtgct gaaagaaact aacggcgtgg ttgccgggcc
    19921 taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg
    19981 gcttggaatt gataaatcgg cattgattta acagatgtgg ttatactcga tcaacattca
    20041 atgaaaggag gcagtatggc agtttcagca agaaatcaat taaccggaac ggttagcgca
    20101 gtagcaatgg gcgctgtgaa tgatgaagta gaactgacgc tggcaggtgg tgcaaaactg
    20161 gtggcaattg tgactcacag cagccagcag gctcttggtc tggcaaaagg gaaagaagcg
    20221 attgccctga ttaaagcccc gtgggtcacg ctggcaacgg aagactgtgg actgaaattc
    20281 tctgctcgca accaatttgc tggcagcgtg tcgacaatca ccgaaggcgc agtgaacgcc
    20341 actgtccata tcaaaacgga tgcgggtttt gaaattgtgg cagtggtaac aaatgaaagt
    20401 caggatgaaa tgaaactcac cactggctcg cgcgttattg cattgatcaa agcatcagcc
    20461 atcctgattg ccaccaaagc gtaaacgcct tatttgccgg agagtatggc tctccggcag
    20521 atttatttct ttggacgata tttttctggc aactccggca ccggacgctt gtcatcgatg
    20581 agatgacgca cggttaagat cggatgacgc cacagcattc tcggcccggc ccaacgcata
    20641 atctgtttca tctcttcacg ctttgcaggc tggtaacagt gcaccggaca ctgcttacag
    20701 gctggtttct cttcgccgaa cacacattta tccagccgct tttgcgcgta ggcaaacagc
    20761 gcgtcgtaat gctccggctc cgctgacgcc tgcgggcatt tcgcttgata aagatcgatc
    20821 atttttttaa tcgtcagttt ttcacgagag atacgcttgc cggacatgct gcctccatct
    20881 cattaagatg catttatatt acaacttaat cttaaagggc actatgactc taaagaagaa
    20941 gggttagcca accgatacaa ttttgcgtac ttgcttcata agcatcacgc aaaagctgca
    21001 aaacagcatc tttcccggaa tcaccatcaa gaactcgccg ttcgcttctt cccctgaaat
    21061 gattaactcc ggtatcatgt gcgccttatg tgattacaac gaaaataaaa accatcacac
    21121 cccatttaat atcagggaac cggacataac cccatgagtg caatagaaaa tttcgatgcc
    21181 catacgccca tgatgcagca gtatctcaag ctgaaagccc agcatcccga gatcctgctg
    21241 ttttaccgga tgggtgattt ttatgaactg ttttatgacg acgcaaaacg cgcgtcgcaa
    21301 ctgctggata tttcactgac caaacgcggt gcttcggcgg gagagccgat cccgatggcg
    21361 gggatcccct accatgcggt ggaaaactac ctcgccaaac tggtgaatca gggcgagtcc
    21421 gttgccatct gcgaacaaat tggcgatccg gcgaccagca aaggtccggt tgagcgcaaa
    21481 gttgtgcgta tcgttacgcc gggcaccatc agcgatgaag ccctgttaca agagcgccag
    21541 gacaatctgc tggcggctat ctggcaggac agcaaaggtt tcggctacgc gacgctggat
    21601 atcagttccg gtcgttttcg cctgagcgaa ccggctgacc gggaaacgat ggcggcagaa
    21661 ctgcaacgca ctaatccggc ggaactgcta tatgcagaag attttgctga aatgtcgtta
    21721 attgaaggtc gtcgcggtct gcgccgtcgt ccgctgtggg agtttgaaat cgacaccgcg
    21781 cgccagcagt tgaatctgca atttggcacc cgcgatctgg tcggttttgg cgtcgagaac
    21841 gcgccgcgcg gactttgtgc tgccggttgt ctgttgcagt atgcgaaaga tactcaacgc
    21901 acgaccctgc cgcatattcg ttctatcacc atggaacgtg agcaggacag catcattatg
    21961 gatgccgcga cacgtcgtaa cctggaaatt actcagaacc tggcgggcgg tgcggaaaac
    22021 acgctggctt cagtgctcga ctgcactgta acgccaatgg gtagtcgtat gctgaaacgc
    22081 tggctgcata tgccagtgcg cgatacccgc gtgttgcttg agcgccagca aactattggc
    22141 gcattgcagg atttcaccgc cgagctacag ccggtgctgc gtcaggtcgg cgacctggaa
    22201 cgtattctgg cacgtctggc tttacgaact gctcgcccac gcgatctggc ccgtatgcgt
    22261 cacgctttcc agcaactgcc ggagctgcgt gcgcagttag aaaatgtcga tagcgctccg
    22321 gtacaggcgc tgcgtgagaa gatgggtgag tttgccgaac tgcgcgatct gctggagcgg
    22381 gccattatcg acacaccgcc ggtactggtg cgcgacggtg gtgttatcgc atcgggctat
    22441 aacgaagagc tggatgagtg gcgcgcgctg gctgacggcg cgaccgatta tctggagcgt
    22501 ctggaagtcc gcgagcgtga acgtaccggc ctggacacgc tgaaagtcgg atttaacgcg
    22561 gtgcatggtt attacattca gatcagccgt ggacaaagcc atctggcacc gattaactac
    22621 atgcgccgcc agacgctgaa aaacgccgaa cgctacatta ttccagagct aaaagagtac
    22681 gaagataaag ttctcacctc aaaaggcaaa gcactggcac tggaaaaaca gctttatgaa
    22741 gagctgttcg acctgctgtt gccgcatctg gaagcgttgc aacagagcgc aagcgcactg
    22801 gcagaacttg atgtgctggt taacctggcg gagcgggcct ataccctgaa ctacacctgc
    22861 ccgaccttca ttgataaacc gggtattcgc atcaccgaag gccgccatcc ggtggttgaa
    22921 caggtactga acgagccatt tatcgccaac ccgctgaacc tgtcgccgca gcgtcgcatg
    22981 ttgatcatta ccggtccgaa tatgggcggt aaaagtacct atatgcgcca gaccgcactg
    23041 attgcgctga tggcctacat cggcagctat gtaccggcac aaaaagtcga gattggacct
    23101 atcgatcgca tctttacccg cgtaggggca gcggatgatc tggcatccgg gcgttcaacc
    23161 tttatggtgg aaatgaccga aaccgccaat attttacata acgccaccga gtacagtctg
    23221 gtgctgatgg atgagattgg gcgtggaacg tccacttacg atggtctgtc gctggcgtgg
    23281 gcgtgcgctg aaaatctggc gaataagatt aaggcattga cgttgtttgc tacccattat
    23341 ttcgagctga cccagttacc ggagaaaatg gaaggcgtgg ctaacgtgca tctcgatgca
    23401 ctggagcacg gcgacaccat tgcctttatg cacagcgtgc aggatggcgc ggcgagcaaa
    23461 agctacggcc tggcggttgc agctctggcg ggcgtgccaa aagaggttat taagcgcgca
    23521 cggcaaaaac tgcgtgagct ggaaagcatt tcgccgaacg ccgccgctac gcaagtggat
    23581 ggtacgcaaa tgtctttgct gtcagtgcca gaagaaacgt cgcctgcggt cgaagcactg
    23641 gaaaatctcg acccggattc actgactccg cgtcaggcgc tggagtggat ttatcgcttg
    23701 aagagtctgg tgtaatatta atgagccccg attaaacttc ggggctcata tatctcttat
    23761 ccgatagata aaaaaggagt aaaataaaca ctattaagaa caaaacattc aagaaaaaaa
    23821 tgagaataac gattgcatca ataattttaa taattagcgc cactgcaata ggcgccgaag
    23881 ataccccttc acattttgat attcccgagc aagtattaaa taatattacg aaggaatcac
    23941 gaaataaact gcaggatatc attgccaagg ctcaacatgg cgataaagcc tcacaaacgg
    24001 tgatgggact catatacctg aatggtctat ctgaaagtcc ttcagtgaag gatataaaca
    24061 aagcatttta ttggctcaac cttgcagcaa agcaaaatta tgatgaagct gaattttact
    24121 taggacagat ttatcattac ggatatgata atacggggcc taactatcgt attgcgatat
    24181 actggtatga gaaagcggca gaaaaaggga atgaacaagc tcaaataaac tgtgcgaata
    24241 tttatcaatt tggcccacag gaattccaaa acattaacaa agccaagtat tggcttcaga
    24301 aagcagtaca gaaggacaat cctattgcac atgcaaactt agggattatc ggaattcaag
    24361 agaataagta tgctgaagct atccctcact taaaaatagc ggcagaaaag ggtcagcgac
    24421 tcggccaata taattacggg acactttttt ttaatggtca gggcgttccc aaagatatac
    24481 gacaggctaa atactggttt gagaaagctg ctactcaaaa cttacctatt gcacaaatta
    24541 ctttaggaca aatttatgcc tgggggcttg atggaaatgg cataaataaa gaagaagcgt
    24601 tgctctggtt aaataaagcc aaagcccaag gtcaatttac tgataaacag atcaacgcca
    24661 ttatttcaaa aaaaatagac taactccaaa ccaaaatgct caaatcaatg ccatctattc
    24721 gctatcacaa aatagaatca tttaattatc gccatatatg ggccgttggt gatattcatg
    24781 gcgattatca attattacaa tcccgcttac accaactctc tttttgcccc gaaactgact
    24841 tacttatttc cgtaggcgat aatattgatc gtgggccgga tagtcttaac gtgctacgcc
    24901 tgttaaacca gccctggttt acatcggtta aaggcaacca cgaggcaatg gcgcttgatg
    24961 ccttcgcgac tggcgatggc aatatgtggc ttgccagtgg cggtgactgg ttctttgagt
    25021 taaatgattc agaacaaaaa gaagcaatag acctgttgct tcgctttcat catcttccgc
    25081 atattataga aatcaccaac gatattataa aatatgttat cgcacatgca gattatccgg
    25141 gtgatgaata tcagtttggc aaggaagtag cggaaagaga ggtactctgg cctgttgatc
    25201 gcgtgcagaa atcactcaaa ggcgagttaa aagaaataaa cggtgccgac tgttttatct
    25261 tcgggcatat gatgtttaca aatatccaga catttgccaa tcaggtttat attgataccg
    25321 gctcgccgga aagcgggcgg ttatcgtttt ataaaataaa atgacaagac cgggtaaggc
    25381 aaagcctcta cccggcactt ttttattaac gcttatctgc caccagcagt ggcggaatgc
    25441 tcggcaccat tggcgcgtca tcaatatctt tctgcgtcat gcggaacgct tcgggataat
    25501 gttcgcggct ggtgcggcgc agcggttcgg tatcgcgcca ggtatagagg caatgctggc
    25561 actggtagac cgtccagaca tcttttaccg gcgatttcgc catcacttca atctgttcat
    25621 cggcacaacg tggacaaatc atcttgttct ccttatttac gtgcggccag catagcggtc
    25681 agtttttcag cccaggcttt ggtttccggt aaatccacca ccggctggct gtagtgacca
    25741 cggttgtccg gggcgacagg cgtggtggcg tcgataatca gcttgtcggt gataccggcc
    25801 gggcttgagc cggggtcgag ttccagcacg gacatattcg gcaactgcac caaatcccct
    25861 gccgggttca ctttcgagga gagcgcccac atcacctgcg gcaggttgaa cggatcaacg
    25921 tcttcatcga ccataatcac catcttcacg tagcccagac cgtgtggcgt ggtcatcgca
    25981 cgcaggccca ccgcgcgggc aaagccaccg tagcgttttt tggtggagat aatcgccagc
    26041 aggccgtggg tgtacatggc gttcaccgcc tgcacttccg ggaactcggc tttcagttgc
    26101 tgatacagcg gcacgcaggt ggctggcccc atcaggtagt cgatttccgt ccacggcatc
    26161 ccgagataga gcgattcaaa aatgggtttg ctgcgataag agactttatc aatgcgcacg
    26221 acggtcatat tgcgcccgcc tgagtagtgg ccggtaaact caccgaatgg cccttcaatc
    26281 tcgcgtttgc ggctttcgat gactccttcg aggatcactt ccgagcccca gggtacatca
    26341 aaaccggtca gtggggcggt ggcaatcggg tacggacttt cgcgcagcgc gcccgccatt
    26401 tcgtactcgg actgatcata tttcagcggc gtggccccca tcagcgtgat gatcggatcg
    26461 ttaccgagcg tgatcgcaat cggcagatct tcaccgcgct cttccgcttt atgcagatgc
    26521 agggcaatat cgtgcatcgg caccggttgc aggccgagct tacgtttgcc tttcacttcc
    26581 atacgataga taccgacgtt ctgcttgccg aagttatccg ggtcgagcgg atcgcgggaa
    26641 actacgcacg ctttgtcgag atagaaaccg ccatcaccgt cgtttaaacg aaacagtggc
    26701 agaatatcga acaggttaat ctcgtcgcca tcgacggtgt tctgcgccca ggctggattg
    26761 gcgcggcgct ccggggcaat ggggaagtta tcccagcggc ggataaactc atcaatctgt
    26821 tttttaaccg gggtgtttgg cggcaggccg agggaaatcg cgtggttctg ccaggaaccg
    26881 atggtgttca ttgccacgcg ggcatcgata aagccgcgaa tattatcaaa ccaaagcgcg
    26941 ggcgcgccgt cgccgatacg cccggtggcg ttggctgcgg ctgccagatc cggttccgcg
    27001 ttcacctctt cgctgatttt cagtaactgg ccgtggtcat caagcgcctg taaaaagctg
    27061 cgtaaatcat caaatgccat tattcattct cctgagaaaa attccgggcc tgcggcaatc
    27121 cttgccagcg cctggcgtga ggatgttcga ggccaaattg atccagcacg cgggctacca
    27181 catggtggac aatgtcatct accgtttcgg gatggttata aaaggcaggc atcggcggca
    27241 ccatcgccac acccatgcgc gaaagtgcga gcatattttc gagatggatt gtgctaagcg
    27301 gcatttcacg cggcaccagc accagtttgc ggccttcttt gagcacgacg tccgccgcgc
    27361 gccctacgag accatcggcg taaccagcgc ggataccggc gagcgttttc atactgcatg
    27421 gaataacgat catgccgtcg gtacgaaagg agccggaaga gatagtcgca gcctgatccg
    27481 ccgggttatg gctgaagtct gcgagggcgg caacatcgcg agcgctgtaa ggcgtttcca
    27541 gttcaatggt ggttttcgcc cacttcgaca tcaccagatg agtctcgaca ttcggcatct
    27601 cccgcagcgc ttgcagtaat gccaccccaa gaggcgcacc ggtagcccct gtcatcccga
    27661 cgatcagtct cattgtttat tctcttaatt gttcgtatac gaacgttatt attaaaatac
    27721 gcctctgttt tcatcgcggc aagcactgaa aagtaacaac atcttcttcg tcactgaaaa
    27781 accgctatga tagcggcaga tagtttgccc ggagtttaca tggcgttacg aaataaagcg
    27841 ttccatcagt tacgacagct ttttcagcag cacacggctc gctggcagca cgagttacct
    27901 gacctcacca aaccacagta cgcggtgatg cgcgccattg ctgataagcc tggtattgaa
    27961 caggtggcac tgatagaagc agcagtcagc accaaagcaa cgctggcaga aatgttggca
    28021 agaatggaaa atcgtggctt agtcagacgt gaacatgacg ccgctgacaa gcggcgacgc
    28081 tttgtctggc tgactgccga aggagaaaag gtacttgctg cggcgatacc gattggtgac
    28141 agtgtggatg cggaattttt ggggcgtttg agtggagaag agcaggagct gtttatgcag
    28201 ctggtgcgca agatgatgag caaataggat gctgattgcc gggtaggacg ctgacgagtc
    28261 ttatccaggc gacaaaacac accgacttcc caaacaaaag aaaaaggcca gcctcgcttg
    28321 agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc
    28381 ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct ggcgaacacg
    28441 ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag
    28501 caaaccgaat cgacgtgcca gcacttcacg ctgtttggcg ttcagctcga acagccattt
    28561 gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt tctctttttc
    28621 atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga
    28681 ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg
    28741 ctctgcgatc tcttccgcac ttggttcgtg gtccagttta tgggacaact cgcgtgcggt
    28801 tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg
    28861 gttcataatc gcccgttcga tcgtctggcg gatccaccag gttgcgtatg ttgagaagcg
    28921 gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaggt tgccctcttc
    28981 gatcaggtcc aacagcgcca gaccacgatt gccataacgg cgggcaatct tcacgaccag
    29041 acgcaggtta ctttcaatca tccggcgacg tgaagcgaca tctccacgca gtgcgcgacg
    29101 cgcgaaataa acttcttctt cggccgttaa cagtggcgaa taaccaatct caccaaggta
    29161 aagctgagtc gcgtccaaca cacgctgtgt ggccccttgc gataacagct cctcttcagc
    29221 caaatcgtta tcactgggtt cctcttcaac taaggctttc tcgtcaaaaa cctcaactcc
    29281 gttctcatca aattccgcgt cttcatttaa atcatgaact ttcagcgtat tctgactcat
    29341 aaggtggctc ctacccgtga tcccttgacg gaactagcaa gcaaaagcct ggttccgccg
    29401 aattatcgct gcggtaaata acgcagcggg tttacggatt tccccttgta acgaatttca
    29461 aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc
    29521 ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa
    29581 tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accggcataa
    29641 acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc
    29701 cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag
    29761 cgccaggtgg agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt
    29821 gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac
    29881 attttgttag cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgacgca
    29941 acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc
    30001 tgggtaatgg catttccgcc agtgattggc gtaccggaag cattacccac ctgtaaggtc
    30061 tgaccgacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa
    30121 tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac ggtataggta
    30181 ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac gatgcgtccg
    30241 ttttccatct gtaccggctg ctgagctact ggctgtactg gctggatttg cggttgttga
    30301 gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgcgc tgtagacgtc
    30361 gttcccattt tcggcggtgg cgtaatcaac ataccagaat tggtatttgc aggcgcattg
    30421 ccattaacgg agcttaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat
    30481 agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc
    30541 atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcacca
    30601 tactgaacac tggaaaagat gttcacgata cgctgacctg cggcaaaata accaggaaaa
    30661 atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac
    30721 ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg
    30781 gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagtgc
    30841 agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg
    30901 ccaaccttgc catccatcgc catgacgggt tgaaacatta tgtaaatcaa gatttttcag
    30961 gcggcgacgc gcctgccact gcaaaccttt aatccgttcg accgagcaaa catgctggac
    31021 aagatgcgcc aggattgccg tttgatatcc cgaaccggtg ccaatttcca gcacccgcga
    31081 ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt
    31141 ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc
    31201 aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc
    31261 ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact
    31321 cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca
    31381 tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg
    31441 gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc
    31501 tgcgggatca ccttatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg
    31561 atttgatcca atggtaaatc cggaacgtta atattaagaa tacgtccggt gcgcagcggc
    31621 tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta
    31681 tgcccgtcaa gcgagacggc cagcgccgga aaacctaaat gacggccttc catcgcggcg
    31741 gctaccgtac cggaataaat aacatcatcc cccagattcg gcccggcgtt aatgccggac
    31801 acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac gcaatcggtc
    31861 ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat
    31921 tccagtgtca gagaatttga agcgccgctg cggttacgat cgggggcgac cacctgaacg
    31981 tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca
    32041 tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct
    32101 ggttgcaaaa ctacccgccg gaagccagaa acggatttct acggtgacgt catcccacca
    32161 gttccagctt aattgttgcg gatacagcag catcgctctg cgcgcggctt caactttttc
    32221 gcgcaccagt aaggcttgta attcagtttc tgcggcgaca gctgcttgtt cgaatgccaa
    32281 cgcttcacgc tgagttcccc attcgccact gcccggcaat gcggcggtta tcatcagctc
    32341 tttatcgttg acgcgacgct gtaattccgc cagttcttcg gtggttgcga caaaccagct
    32401 accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt
    32461 gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact
    32521 ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa
    32581 gttgctaccg ccaatcccga aacgttgggc accgaagtag ttcggtacac cttttacgca
    32641 aatatcgatc agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt
    32701 aaaggcgtta cctttcagcg cccctaaacg cagcttgcgc ttgtgccgca catactccag
    32761 cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc ccggcacgcg
    32821 agcacataac cactgttccg ttacagcatg tttgtctttt tgcccagcga agctgacttc
    32881 acgggcatga attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc
    32941 gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc
    33001 ttccaccacc acaaagtctt ccggattggc tttcagcaac ccggtgcctt gcggtttacc
    33061 gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc
    33121 gcttcacagg caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct
    33181 ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt
    33241 ggtatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgcccagc
    33301 gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctgtcggca
    33361 cctttaaatg ccggatcggt atccgggaac agcttgccga tatcccccag cgccgccgcg
    33421 ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc catcagaatg cgccagcaat
    33481 cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggcg
    33541 tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga
    33601 ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta tccgcgcggc
    33661 cttcgaccaa ctgcggatga aatccgcaat attccagcgc cgaggcttcg tcggtaatgg
    33721 tagcgccttc atttagagcg cgcgtcaggc agtcatgtaa cagctcacga gggaaaaatt
    33781 gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaata gcatttttgc
    33841 ccggttcggc acgtttcatc gtatcgcgca ctggtgcggc gaggatcccc cccgtgcggc
    33901 tggtttcgct caacgtcaac aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg
    33961 cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt cagacctgcc agcaccgaat
    34021 cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt
    34081 gtgcaaaacg gctatcgcca ggacttatgg caatgacgac acgtttcacc cggggatgcg
    34141 ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct
    34201 taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat
    34261 ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag
    34321 actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca
    34381 tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa
    34441 tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg
    34501 ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat
    34561 actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg
    34621 aaaaacggca tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac
    34681 cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca
    34741 gaacataatc tcattattag ttacggtttg aattatgaac agaggagaca agaaagtaca
    34801 aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt catcagggtg
    34861 aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact
    34921 actgcgacag gcatcagtgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg
    34981 ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat aaaaccgacc
    35041 acggcaccag gcagtgacca tgtggtttct tcatcctcag taaggctgtc gttatttgtt
    35101 agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcatgg
    35161 aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg
    35221 ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg attcaggcgc
    35281 ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata
    35341 taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa atacttcgat
    35401 aaagcgccct tctcctacgc gttcacgaac catctggcgt tcggcgcggt gtggcgagat
    35461 gaatgcggtc agcaccacca gtccggcttc aaccatcaaa ttcgccactt caccgacgcg
    35521 acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg
    35581 aacattgtcg ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag
    35641 cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc
    35701 acgatgaccg tggtgtagct cgcgttgttg tacagtgact ggatggctat gccagacgac
    35761 gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgtgcgcc ccagtgcgga
    35821 aagtggcgac gaaccagggc attcaattcc agttcaaatg cactgaattc agatggcgca
    35881 gcagttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac attgctcagg
    35941 cgatcgataa aaatcagccc accggtaacc ggattttgct gataacgatc taacaccagt
    36001 ggctcgtcaa aagtgagatc caccaggccg atgccgttca gcggcaggtt ttcaacttcg
    36061 cgttgggtaa ggttattaat atcaacctga taacgaatac catcaacacg agcacgcgtc
    36121 ttcttaccgg caattttgat gtcataactc tggcccgcgg aaagcggctg ttccgccatc
    36181 cataccacat ccaccgacgc gctctgcaca gccggtaacg cttcgtctgc cgccagcagc
    36241 agatcgccac ggctgatgtc gatctcatcc gtcagcacta gggtgatcgc ttctccggca
    36301 aaggcttctt cgcgatcgcc atcaaaagtc acgatccgcg cgacgtttga ttccacacca
    36361 gagggcagca ctttgacacg ttgcccgact tccactcgac cggatgccag cgttccggcg
    36421 tagccacgaa aatcgagatt cgggcgatta acgtactgca ccgggaagcg cattggctgg
    36481 gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt cggaccgctg
    36541 taccacggca tactttcact ttgcgaagcc acgttgtcgc cttccagtgc agagagcggc
    36601 acaaagcgga tatccagatt acccggcagc tgcccggcaa acgtcagata atcttcgcga
    36661 atacgggtga acgtctcttc actgtaaccc accagatcca ttttgttgat cgccacgacc
    36721 agatgtttga tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg
    36781 cctttacggg catcgatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata
    36841 ttgcgagtgt actgctcgtg ccctggggta tcggcgataa taaatttacg cttctcggta
    36901 gagaaataac ggtaagccac gtcaatggtg atgccctgtt cgcgctcagc ttgcaggccg
    36961 tccaccagca gagccagatc cagcttttcg ccctgggtgc cgtgacgctt actgtcgtta
    37021 tgcagcgatg agagctgatc ttcgtagatt tggcgggtat cgtgcagcag acgaccaatc
    37081 agggtacttt ttccgtcatc gacgctacca caagtcagaa aacgcagcag gcttttatgt
    37141 tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg
    37201 gtgttcatct taaaaatacc cctgacgttt tttcaactcc atggatcccg cctggtcgcg
    37261 gtcaatcacg cgaccctgac gttcactggt ggtggaaacc agcatctctt cgatgatttc
    37321 cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg
    37381 gaaacgcacc atccgttttt taatcacttc tcccggttgc aggtcgatac ggttgtcatc
    37441 aatcatcatc aacataccgt cgcgttccag aaccggtcgt tccgcagcga gatacagtgg
    37501 aacaatgtca atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga
    37561 gagcgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag
    37621 ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtagattcg
    37681 ctctttagcg cgggatttct cttcgtcgcg gcgcgcacca ccgaaggcgg catcaaaacc
    37741 gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct
    37801 gccatgaacg aatggattaa tccccatcgc tacgccttcc gggtttttat gcaccagcag
    37861 ctcgcagccg taggctttag ccgtacgatc gcggaactca tacatctcgc ggaatttcca
    37921 gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc
    37981 cagatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa
    38041 ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt
    38101 aagtcgtttt tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg
    38161 tttcgactat agggagcgca ggagaacgaa tgaaattacc aattagaatg agtagttcct
    38221 taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg
    38281 gcgtttagga agatttgaaa ttgttttagc gcatcggcag tttcatacta tggcggtaaa
    38341 aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg
    38401 ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcatct aaacctggcg
    38461 attttgctaa tactcaggca cgacatattg ctactttctt tccgggacgc atgaccggaa
    38521 ctccagcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc
    38581 gcagtgatat tcggacattt aatagtcggt atatttatac cgcccgcgat aatcgcaaga
    38641 gctggcataa cgtgacggga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc
    38701 agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct gacgccgatg
    38761 ccaatctcgg cgggctgacg ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca
    38821 tgctggaatt ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg
    38881 cgaccagtgg tgaagaggaa gggaaattag gtgctgagaa tttactcaag cggatgagtg
    38941 acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata
    39001 aattgtattt caacagcggc gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca
    39061 gggcgctggc aattgcgcgt agtcatggaa ttgccgcaac gaccaatccg ggtttgaata
    39121 aaaattatcc gaagggtact ggctgttgta atgacgccga agttttcgac aaagccggta
    39181 ttgccgtact ttcggtagaa gcaacaaatt ggaatcttgg gaataaggat ggttatcagc
    39241 aacgcgcaaa aacagctgca ttccctgcgg gaaatagctg gcatgacgta agactggata
    39301 atcaacaaca tattgataag gcacttccag gaagaataga acgtcgctgc cgtgatgtta
    39361 tgcggataat gctaccgctg gtgaaggagc tggcgaaggc gtcttgatgg gtttgaaaat
    39421 aggagctggg agttctaccg caggggcggg gaactccaag tgatgtccat catcgcatcc
    39481 agtgcgtccg gtttatcccc gctgacgcgg ggaacacaca tgaatgtcgg ttcagaccgt
    39541 gtttttaccc ggtttatccc cgctggcgcg gggaacactg cctcggcggc ggctctgcgc
    39601 aaccgcgaaa cggtttatcc ccgctggcgc ggggaacacg ttaaaccgtg atgatcagca
    39661 aaagctattt ccggtttatc tccgctgacg cggggaacac gtcaataggc ggcgtcccgt
    39721 agccgtcccc ttcggtttat ccccgctggc gcggggaaca cttgaaatgg tccagcactg
    39781 cgaccgtcgg ccccggttta tccccgctgg cgcggggaac acgctggtgg cgcgggcaaa
    39841 cggaacaatc ccgccggttt atccccgctg gcgcggggaa cacctggatt tcacctcagc
    39901 aaatgctgga tgtggcggtt tatccccgct ggcgcgggga acaccacccc tgatattttt
    39961 gccaccgtgg ttactccggt ttatccccgc tggcgcgggg aacacagaaa tcaaactcag
    40021 cgtgcaaatg gggtagccgg tttatccccg ctggcgcggg gaacacttgc gttatttcta
    40081 tctggtgagt cgcggcaccg gtttatcccc gctggcgcgg ggaacacgta ttttttatcg
    40141 tcgcatgagt cgcggtaacc ggtttatccc cgctggcgcg gggaacactt ctgccgcgcc
    40201 agcaaacgta tcggcggtca cggtttatcc ccgctggcgc ggggaacacc ccaggccaag
    40261 cattatatcc tgtgcgtcgt tcggtttatc cccgctggcg cggggaacac accaagaagc
    40321 tgacgaatca gcccgacacg cacggtttat ccccgctggc gcggggaaca cccagtttca
    40381 tatgagccgc cg
//
LOCUS       DAYPHN010000023        40227 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000023 DAYPHN010000000
VERSION     DAYPHN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.023"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            209..2359
                     /gene="btsT"
                     /locus_tag="ACG0BW_001225"
     CDS             209..2359
                     /gene="btsT"
                     /locus_tag="ACG0BW_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFK7258215.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVIPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2477..2680
                     /gene="yjiX"
                     /locus_tag="ACG0BW_001226"
     CDS             2477..2680
                     /gene="yjiX"
                     /locus_tag="ACG0BW_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFK7258216.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2691..3647
                     /gene="yjiA"
                     /locus_tag="ACG0BW_001227"
     CDS             2691..3647
                     /gene="yjiA"
                     /locus_tag="ACG0BW_001227"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFK7258217.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(3693..4607)
                     /gene="mrr"
                     /locus_tag="ACG0BW_001228"
     CDS             complement(3693..4607)
                     /gene="mrr"
                     /locus_tag="ACG0BW_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HFK7258218.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     SKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQEM
                     NHLAFNFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            4795..8307
                     /gene="hsdR"
                     /locus_tag="ACG0BW_001229"
     CDS             4795..8307
                     /gene="hsdR"
                     /locus_tag="ACG0BW_001229"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HFK7258219.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQARE
                     KAQTQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQIITRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDNLLDKFSDYIWDELA"
     gene            8509..10098
                     /gene="hsdM"
                     /locus_tag="ACG0BW_001230"
     CDS             8509..10098
                     /gene="hsdM"
                     /locus_tag="ACG0BW_001230"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HFK7258220.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPEGYRWDDLKSRIGQEQLQFYRKMLVHLGEDDKKLVQAVFHNVSTTITEPKQ
                     ITALVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVFGEDPHGLSPRTEGEWSFNAEETEIADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALSELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            10095..11462
                     /gene="hsdS"
                     /locus_tag="ACG0BW_001231"
     CDS             10095..11462
                     /gene="hsdS"
                     /locus_tag="ACG0BW_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     specificity subunit"
                     /protein_id="HFK7258221.1"
                     /translation="MSAGKLPEGWEQIEIGDIADVISGGTPKSGVAENFAPSGEGVAW
                     LTPADLSGYKEKYISHGARDLTTLGYSSCSAKLMPKGTILFSSRAPIGYVAIAANEIA
                     TNQGFKSFAFPSDIFPDYAYYFLRNIRHIAEEMGTGTTFKEISGSSAKTLPFVLVPFA
                     EQKIIAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLGAAVRGKLTEDWRDNSSLSG
                     WREGKLGEFIKKPSYGTSSKSNKEGLIPVLRMGNLQGGKLDWTDLVYTSDTIEIEKYK
                     LEYNDVLFNRTNSPELVGKTAIYKSEQPAIYAGYLIRVQCLPDLNPDYLNYHLNSILG
                     RQYCYSVKSDGVSQSNINAQKLIAYPITVPPLPEQAEIVRRVEQLFAYADTIEKQVNN
                     ALARVNNLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKA
                     SRKKS"
     gene            11691..12032
                     /gene="symE"
                     /locus_tag="ACG0BW_001232"
     CDS             11691..12032
                     /gene="symE"
                     /locus_tag="ACG0BW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFK7258222.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAIDVKVMEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(12079..13242)
                     /locus_tag="ACG0BW_001233"
     CDS             complement(12079..13242)
                     /locus_tag="ACG0BW_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFK7258223.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPALPEEVLAVYRAQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(13290..14172)
                     /locus_tag="ACG0BW_001234"
                     /pseudo
     CDS             complement(13290..14172)
                     /locus_tag="ACG0BW_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(14270..14350)
                     /locus_tag="ACG0BW_001235"
     CDS             complement(14270..14350)
                     /locus_tag="ACG0BW_001235"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258224.1"
                     /translation="MQTRCVLQQVRDERLKDIGLCREDVV"
     gene            complement(14578..15498)
                     /locus_tag="ACG0BW_001236"
     CDS             complement(14578..15498)
                     /locus_tag="ACG0BW_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFK7258225.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPEREPYSWLKS"
     gene            15683..16963
                     /gene="yjiN"
                     /locus_tag="ACG0BW_001237"
     CDS             15683..16963
                     /gene="yjiN"
                     /locus_tag="ACG0BW_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFK7258226.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDHATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            17079..18230
                     /gene="yjiM"
                     /locus_tag="ACG0BW_001238"
     CDS             17079..18230
                     /gene="yjiM"
                     /locus_tag="ACG0BW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFK7258227.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTAHVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            18240..19007
                     /gene="yjiL"
                     /locus_tag="ACG0BW_001239"
     CDS             18240..19007
                     /gene="yjiL"
                     /locus_tag="ACG0BW_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFK7258228.1"
                     /translation="MTYSIGIDSGSTTTKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            19004..19261
                     /gene="ytiA"
                     /locus_tag="ACG0BW_001240"
     CDS             19004..19261
                     /gene="ytiA"
                     /locus_tag="ACG0BW_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFK7258229.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            19326..20186
                     /gene="yjiK"
                     /locus_tag="ACG0BW_001241"
     CDS             19326..20186
                     /gene="yjiK"
                     /locus_tag="ACG0BW_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFK7258230.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQEHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            20254..21432
                     /gene="yjiJ"
                     /locus_tag="ACG0BW_001242"
     CDS             20254..21432
                     /gene="yjiJ"
                     /locus_tag="ACG0BW_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7258231.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTAQLFKLQVVTS
                     "
     gene            complement(21445..21998)
                     /gene="kptA"
                     /locus_tag="ACG0BW_001243"
                     /pseudo
     CDS             complement(21445..21998)
                     /gene="kptA"
                     /locus_tag="ACG0BW_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            22248..22931
                     /gene="yjiH"
                     /locus_tag="ACG0BW_001244"
     CDS             22248..22931
                     /gene="yjiH"
                     /locus_tag="ACG0BW_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFK7258232.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            22928..23389
                     /gene="yjiG"
                     /locus_tag="ACG0BW_001245"
     CDS             22928..23389
                     /gene="yjiG"
                     /locus_tag="ACG0BW_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFK7258233.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            23402..24574
                     /gene="iadA"
                     /locus_tag="ACG0BW_001246"
     CDS             23402..24574
                     /gene="iadA"
                     /locus_tag="ACG0BW_001246"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFK7258234.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFTRKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            24639..25550
                     /gene="hypT"
                     /locus_tag="ACG0BW_001247"
                     /gene_synonym="qseD"
     CDS             24639..25550
                     /gene="hypT"
                     /locus_tag="ACG0BW_001247"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFK7258235.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(25543..25935)
                     /gene="iraD"
                     /locus_tag="ACG0BW_001248"
     CDS             complement(25543..25935)
                     /gene="iraD"
                     /locus_tag="ACG0BW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFK7258236.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(25932..26015)
                     /gene="idlP"
                     /locus_tag="ACG0BW_001249"
     CDS             complement(25932..26015)
                     /gene="idlP"
                     /locus_tag="ACG0BW_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFK7258237.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(26131..26274)
                     /gene="ytiD"
                     /locus_tag="ACG0BW_001250"
     CDS             complement(26131..26274)
                     /gene="ytiD"
                     /locus_tag="ACG0BW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFK7258238.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            26608..27438
                     /gene="yjiC"
                     /locus_tag="ACG0BW_001251"
     CDS             26608..27438
                     /gene="yjiC"
                     /locus_tag="ACG0BW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFK7258239.1"
                     /translation="MSMPLSNALQSQIITDAHFLHHPIVDSEFTRKLKYARMDSENIY
                     LPPLTRGNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(27579..28352)
                     /gene="uxuR"
                     /locus_tag="ACG0BW_001252"
     CDS             complement(27579..28352)
                     /gene="uxuR"
                     /locus_tag="ACG0BW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFK7258240.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(28567..30027)
                     /gene="uxuB"
                     /locus_tag="ACG0BW_001253"
     CDS             complement(28567..30027)
                     /gene="uxuB"
                     /locus_tag="ACG0BW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFK7258241.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETIQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTVTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(30108..31292)
                     /gene="uxuA"
                     /locus_tag="ACG0BW_001254"
     CDS             complement(30108..31292)
                     /gene="uxuA"
                     /locus_tag="ACG0BW_001254"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFK7258242.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIIEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            31632..32975
                     /gene="gntP"
                     /locus_tag="ACG0BW_001255"
     CDS             31632..32975
                     /gene="gntP"
                     /locus_tag="ACG0BW_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFK7258243.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(33218..34120)
                     /gene="fimH"
                     /locus_tag="ACG0BW_001256"
     CDS             complement(33218..34120)
                     /gene="fimH"
                     /locus_tag="ACG0BW_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFK7258244.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(34140..34583)
                     /locus_tag="ACG0BW_001257"
     CDS             complement(34140..34583)
                     /locus_tag="ACG0BW_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7258245.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(34656..35186)
                     /gene="fimF"
                     /locus_tag="ACG0BW_001258"
     CDS             complement(34656..35186)
                     /gene="fimF"
                     /locus_tag="ACG0BW_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFK7258246.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEKQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(35196..37832)
                     /gene="fimD"
                     /locus_tag="ACG0BW_001259"
     CDS             complement(35196..37832)
                     /gene="fimD"
                     /locus_tag="ACG0BW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFK7258247.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(37899..38624)
                     /gene="fimC"
                     /locus_tag="ACG0BW_001260"
     CDS             complement(37899..38624)
                     /gene="fimC"
                     /locus_tag="ACG0BW_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFK7258248.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(38661..39200)
                     /gene="fimI"
                     /locus_tag="ACG0BW_001261"
     CDS             complement(38661..39200)
                     /gene="fimI"
                     /locus_tag="ACG0BW_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7258249.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(39265..39813)
                     /gene="fimA"
                     /locus_tag="ACG0BW_001262"
     CDS             complement(39265..39813)
                     /gene="fimA"
                     /locus_tag="ACG0BW_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFK7258250.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAAGFNIQLNDCDTNVASKAAVAFLGTAID
                     VGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         9885 a        10363 c         9948 g        10031 t
ORIGIN      
        1 tatggtcact taaacgacgc ctttgccgct caccgccatt cctgaccgct tacgtcccta
       61 aaataaccac tcagttattt accttacttt acgcgcgcgt aactctggca acatcactgc
      121 aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc ttcggccaac
      181 tattaatcaa tacatgccag gttttactat ggatactaaa aagatattca agcacatacc
      241 ctgggtgatt ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg
      301 ggagcacgtc agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta
      361 tcgctactac agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac
      421 gcctgcggtt attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt
      481 tggtcaccac ttcgccgcta tcgccggtgc tggtccgctg gtgggtccgg ttctcgccgc
      541 gcagatgggc tacctgcctg gcacgctgtg gctgctggcg ggggtcgtgc tggccggtgc
      601 ggttcaggac tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctcttggtga
      661 gatgatcaaa gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt
      721 aatcatgatc atcatcctcg ccgttctggc actgattgtg gttaaagccc tggccgaaag
      781 tccgtggggg gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta
      841 catgcgcttt attcgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct
      901 ggttgcctct atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact
      961 gacctttaaa gataccacca ttactttcgc gctgattggc tatgcgtttg tttccgcact
     1021 gctgccagtg tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg
     1081 cgttatcgtc ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc
     1141 catgacccag tacattgacg gtactggccc gctgtggaaa ggcgctctgt tcccgttcct
     1201 gttcatcacc atcgcctgtg gtgcggtatc tggcttccac gcgctgatct cttccggtac
     1261 gacgccgaaa ctgctggcta atgaaaccga cgcgcgtttc atcggctacg gcgcaatgct
     1321 gatggagtcc ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct
     1381 ttacttcgcg atgaacaccc cgcctgctgg cctcggcatc accatgccta acctgcatga
     1441 aatgggtggc gagaacgcgc cgatcatcat ggcacagctg aaagacgtta ccgcacacgc
     1501 ggcagcgacc gtcagctcct ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc
     1561 gaaagacatt ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt
     1621 aggtatcgct cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca
     1681 cttcggtatt ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc
     1741 tggccgcttt atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga
     1801 ctctctggtt gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct
     1861 gtatcagggc gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat
     1921 ctccaaccag atgctggcag ccgtggcgct ggtactgggc accgttgtac tgattaagat
     1981 gaagcgcacc caatacatct gggttaccgt tatcccggca gtatggctgc ttatctgcac
     2041 cacctgggcg ctgggcctga aactgttcag caccaacccg cagatggaag gcttcttcta
     2101 catggcgagc cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat
     2161 tgccaacatg aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt
     2221 cctgattgtg gtgtacagca tcatcttcta cggtttcaaa acctggctga aggtgcgtaa
     2281 cagcgacaaa cgtactgaca aagaaacacc gtacgttcca atcccggaag gcggcgtgaa
     2341 gatctcttcg caccactaac cggttgcctt atccggctta caaaatgtct atcgtaggcc
     2401 ggataaggcg tttacgccgc atccggcaaa tccccagccc ggaccttccg ggctttacgc
     2461 atatctggaa ggcaaaatgt ttggtaactt aggacaggca aaaaaatatc tcggccaggc
     2521 ggcgaagatg ttgattggca ttccagacta cgacaactac gtcgagcata tgaagaccaa
     2581 ccatcccgac aagccgtaca tgagctatga agaattcttc cgcgaacgcc agaacgcgcg
     2641 ctacggcggc gatggtaaag gcggtatgcg ctgttgttaa tggagagagc atgaacccga
     2701 ttgcagttac cctactcacc ggttttttag gcgcaggaaa aaccaccctg ctgcgccata
     2761 ttcttaacga acaacacggc tacaagattg ccgtgattga aaacgaattc ggcgaagtct
     2821 ccgttgatga tcaactgatt ggcgaccgcg ccacgcagat caaaacgctg accaacggct
     2881 gcatctgctg ttcgcgctcc aacgagctgg aagacgccct actcgacctg ctggataatc
     2941 tcgacaaggg caatattcag ttcgaccgtc tggtcattga atgcaccggc atggccgatc
     3001 ccggcccgat tattcagacc tttttctccc atgagatttt atgccagcgt tacctgctgg
     3061 atggcgtgat tgcgctggtg gatgcggtac atgccgatga gcagatgaac cagttcacca
     3121 tcgcccagtc ccaggttggc tacgccgacc gtattctgct gactaaaacc gacgtcgcag
     3181 gcgaagcaga aaaactgcgt gaacgcctgg cgcgcatcaa cgcccgtgca ccggtctaca
     3241 ccgtcaccca cggcgacatc gacctgggcc tgttgttcaa caccaacggt tttatgctgg
     3301 aagaaaacgt cgtcagcacc aagccacgtt ttcactttat cgcggacaaa caaaacgata
     3361 tttcgtcgat tgtggtggaa ctggattacc cggtagatat cagcgaagtt tcccgcgtga
     3421 tggaaaacct gctgctggaa tcggcagata aactgctgcg ttacaaaggg atgctgtgga
     3481 ttgacggcga acctaaccgc ctgctgttcc agggcgtcca gcgcctctac agcgccgact
     3541 gggacaggcc gtggggcgat gaaaaaccgc atagcacgat ggtgtttatc gggattcagc
     3601 tgccggagga tgagattcgg gcggcgtttg cggggttgag gaagtaaggg gttatgggcc
     3661 ggataaggcg cagccgcatc cggcctgata tttcactcaa aataatccat atccagtttc
     3721 ggcaccttca acaaacgtga agaaacccct acttcgtttt cgatcattaa gtgcaccagg
     3781 cgttccccat caaccaacac cataccctcg acggattggg caaagtcacg cgcctgagaa
     3841 gtaaatccag aagtggtaat aaacacccca cgtttcgctt tttgcccagc cagtgcgccg
     3901 taaaatgcct gtaattctgg cctgcctaca gtattctgcc aacgttttgc ctgaacataa
     3961 actttctcca ggccaagttt atcaagcgat atcacaccat cgatgccacc atctccagta
     4021 ccgccaacac gctgcaaatc atcacggtgg ccgccatacc ccaggcgatg caaaacatcc
     4081 agaacaatga cttcaaagcg cgaaggagaa acctgcaata agttttccag aacctcatca
     4141 gccaccgcat cacgaagctc ttttagcgcc tgatctaacc gatcgtccgg gctgctcttt
     4201 gcaagttctt catgatcggg agagtcggct ttcggatcta aatcgacggc atccggccgt
     4261 gacttaagtt tgacattcac aaaattgaag gccagatggt tcatctcctg ctccgtcatt
     4321 ggctggggat gagacgcaac ccagtcaaaa cccgcaggag tcaggcacca tttgccacgc
     4381 gacaaacttt gcgacaaccc ggcacgtttt aaacggtcat gtgcccagcc tgcacgattt
     4441 ttataaacaa gttgtccgct ggtaatgact ttcgatcgct ggctgtcatc cagtcctaat
     4501 gcatccgcgg cagcctcatg aacatcacgc gcggctgcac cttccggttt tgttgccaga
     4561 taacgcagaa caggttcaat aaatttgtca taggtaggaa ccgtcatagt acatccttgc
     4621 agaatcaggt agatgttttt cggctactat aacactacca aaatagacga acacgttaga
     4681 aatgagtcag ttgctgtgac tccggtcatt gcccggaaag gtacagaaag ctaagatagg
     4741 gcgttatggg ccttaaatat ttggacaggc ccacacagca atggattaat aacaatgatg
     4801 aataaatcca acttcgaatt cctgaagggc gtcaacgact tcacttatgc catcgcctgt
     4861 gcggcggaaa ataactaccc ggatgatccc aacacgacgc tgattaaaat gcgtatgttt
     4921 ggcgaagcta cagcgaaaca tcttggtctg ttactcaaca tccccccttg tgagaatcaa
     4981 cacgatctcc tgcgcgaact cggcaaaatc gccttcgttg atgacaacat tctctctgta
     5041 ttccacaaat tacgccgcat tggtaaccag gcggtgcacg aataccataa cgatctcgac
     5101 gatgcccaga tgtgcctgcg actcgggttc cgcctggctg tctggtacta ccgtctggtc
     5161 accaaagatt atgacttccc ggtgccggta tttgtgttgc cggaacgtgg cgaaaacctc
     5221 tatcaccagg aagtgctgac gctaaaacaa cagcttgaac agcaggcgcg agaaaaagcg
     5281 cagactcagg cagaagtcga agcgcaacag cagaagctgg ttgccctgaa cggctatatc
     5341 gccattctgg aaggcaaaca gcaggaaaca gaagcgcaaa cccaggctcg ccttgcggcg
     5401 ctggaagcac agctcgccga gaagaacgcg gagctggcca aacagaccga acaggaacgt
     5461 aaggcttacc acaaagaaat taccgatcag gccatcaagc gcacactcaa ccttagcgaa
     5521 gaagagagtc gcttcctgat tgacgcgcaa ctgcgtaaag caggctggca ggccgatagc
     5581 aaaaccctgc gcttctccaa aggcgcacgc ccggaacccg gcgtcaataa agccattgcc
     5641 gaatggccga ccgggaaaga tgaaacgggt aatcagggct ttgcggatta tgtgctgttt
     5701 gtcggcctca agcccatcgc ggtggtagag gcgaaacgta aaaatatcga cgttcccggc
     5761 aagctcaatg agtcgtatcg ctacagcaaa tgcttcgata atggcttcct gcgggaaacc
     5821 ttgcttgagc attactcacc ggatgaagtg catgaagctg tgccggagta tgaaactagc
     5881 tggcaggaca ccagcggcca acaacggttt aaaatcccct tctgctactc caccaacggg
     5941 cgcgaatatc gcgcagcaat gaagactaaa agtggcatct ggtatcgcga cgtgcgtgat
     6001 acccgcaata tgtcgaaagc cttacccgag tggcatcgcc cggaagagct gctggaaatg
     6061 ctcggcagcg aaccgcaaaa acagaatcag tggtttgccg ataacccggg catgagcgag
     6121 ctgggcctgc gttattacca ggaagatgcc gtccgcgcgg tggaaaaggc aatcgtcaag
     6181 ggtcaacaag agatcctgct ggcgatggcg accggtaccg gtaaaacccg caccgcaatt
     6241 gccatgatgt tccgcctgat ccagtcccag cgttttaaac gcattctctt ccttgtcgat
     6301 cgccgttctc ttggcgagca ggcactgggg gcgtttgaag atacgcgtat taacggcgac
     6361 accttcaaca gcattttcga cattaaaggg ctgacggata aattccccga agacagcacc
     6421 aaaattcacg ttgccaccgt acagtcgctg gtgaaacgca ccctacaatc agatgaaccg
     6481 atgccggtgg cccgttacga ctgtatcgtc gtcgatgaag cgcatcgcgg ctatattctc
     6541 gataaagagc agaccgaagg cgagctgcag ttccgcagcc agctggatta cgtttctgcc
     6601 taccgtcgca ttctcgatca cttcgatgcg gtaaaaatcg ccctcaccgc caccccggcg
     6661 ctgcatacgg tgcagatttt cggcgagccg gtttaccgtt atacctaccg taccgcggtt
     6721 atcgacggtt ttctgatcga ccaggatccg ccgattcaga tcatcacccg caacgcccag
     6781 gagggggttt atctctccaa gggcgaacag gtagagcgca tcagcccgca gggagaagtg
     6841 atcaacgata ccctggaaga cgatcaggat tttgaggtcg ccgactttaa ccgtggcctg
     6901 gtgatcccgg cgtttaaccg cgccgtctgt aacgagctca ccaactacct tgacccgacc
     6961 gggtcgcaaa aaacgctggt cttctgcgtt accaatgccc atgccgatat ggtggtggaa
     7021 gagttgcgta ccgcgttcaa gaaaaagtat ccgcaactgg agcacgacgc gatcatcaag
     7081 atcaccggtg atgccgataa agacgcgcgc aaagtgcaga ccatgatcac ccgcttcaat
     7141 aaagagcggc tgcccaatat cgtggtaacc gtcgacctgc tgacaaccgg cgtcgatatt
     7201 ccgtcgatct gtaatatcgt gttcctgcgt aaagtacgca gccgtattct gtacgagcag
     7261 atgaaaggcc gcgccacgcg cttatgcccg gacgtgaata aaaccagctt taagattttc
     7321 gactgtgtcg atatctacag cacgctggag agcgtcgaca ccatgcgtcc ggtggtggtg
     7381 cgtccgaagg tggaactgca aacgctggtc aatgaaatta ccgattcaga aacctataaa
     7441 atcaccgaag cggatggccg tagctttgcc gagcacagcc atgaacaatt ggtggcgaag
     7501 ctccagcgca tcatcggcct ggccacgttt aaccgtgacc gcagcgaaac gatagataaa
     7561 caggtgcgtc gtctggatga gctatgccag gacgcggcgg gcgtgaactt taacggcttc
     7621 gcctcgcgcc tgcgggaaaa agggccgcac tggagcgccg aagtctttaa caaactgcct
     7681 ggctttatcg cccgtctgga aaagctgaaa acggacatca acaacctgaa tgatgcgccg
     7741 atcttcctcg atatcgacga tgaagtggtg agtgtaaaat cgctgtacgg tgattacgac
     7801 acgccgcagg atttcctcga agcctttgac tcgctggtgc aacgttcccc gaacgcgcag
     7861 ccggcattgc aggcggtgat caatcgcccg cgcgatctca cccgtaaagg gctggtcgag
     7921 ctccaggagt ggtttgaccg ccagcatttt gaggaatctt ccctgcgcaa agcatggaaa
     7981 gagacgcgca atgaagatat cgccgcccgg ctgattggtc atattcgccg cgctgcggtg
     8041 ggcgatgcgc tgaaaccgtt tgaggaacgt gtcgatcacg cgctgacgcg cattaagggt
     8101 gaaaacgact ggagcagcga gcaattaagc tggctcgatc gtttagcgca ggcgctgaaa
     8161 gagaaagtgg tgctcgacga cgatgtcttc aaaaccggca acttccaccg ccgcggcggt
     8221 aaggcgatgc tacaaagaac ctttgacgat aatctcgaca acctgcttga taaattcagc
     8281 gattatatct gggacgagct ggcctgacac gtatacactt catccttcag gctgcctctg
     8341 cgttggctgc gctcgttcac cccggtcacg tacttctgta cgctcccggg gattcactca
     8401 cttgccgcct tgatgcaacc tgaatgattt tgtgtatatt accctcggca atttcttctt
     8461 ctgcggcccc aaacaatgcg ggccgttgtt taatttacgg aactcacaat gaacaataac
     8521 gatctggtcg cgaagctgtg gaagctgtgc gacaacctgc gcgatggcgg cgtttcctat
     8581 caaaactacg tcaatgaact cgcctcgctg ctgtttttga aaatgtgtaa agagaccggg
     8641 caggaagcgg aatacctgcc ggaaggttac cgctgggatg acctgaaatc ccgcatcggc
     8701 caggagcagt tgcagttcta ccgaaaaatg ctcgtgcatt taggcgaaga tgacaaaaag
     8761 ctggtacagg cagtttttca taatgttagt accaccatca ccgagccgaa acaaataacc
     8821 gcactggtca gcaatatgga ttcactggac tggtacaacg gtgcgcacgg taagtcgcgc
     8881 gatgacttcg gcgatatgta cgaagggctg ctgcagaaga acgccaatga aaccaagtct
     8941 ggcgcgggcc agtacttcac cccgcgtccg ctgattaaaa ccattattca tctgctgaaa
     9001 ccgcagccac gtgaagtggt acaggatccg gcagccggta cggcgggctt tttgattgaa
     9061 gccgaccgct acgttaagtc gcagactaac gatctggacg accttgatgg cgacacgcag
     9121 gatttccaga tccaccgcgc gtttatcggc ctcgaactgg tgcccggcac ccgtcgtctg
     9181 gcgctgatga actgcctgct gcacgatatt gaaggcaacc tcgaccacgg cggcgcaatc
     9241 cgtctgggca acaccctggg cagcgacggc gaaaacctgc cgaaggcgca tattgtcgcc
     9301 actaacccgc cgtttggcag cgccgcaggc accaacatta cccgcacctt tgttcacccg
     9361 accagcaaca aacagttgtg ctttatgcag catattatcg aaacgctgca ccccggcggt
     9421 cgtgcggcgg tggtggtgcc ggataacgtg ctgtttgaag gcggcaaagg tacggacatc
     9481 cgtcgtgacc tgatggataa gtgtcatctg cacactattc tgcgtctgcc gaccggtatt
     9541 ttttacgcgc agggcgtgaa gaccaacgtg ctgttcttta ccaaagggac ggtggcgaac
     9601 ccgaatcagg ataagaactg caccgatgac gtgtgggtgt acgacctgcg gaccaatatg
     9661 ccgagctttg gtaaacgcac gccgtttacc gacgagcatt tgcaaccgtt tgagcgcgtg
     9721 tttggcgaag acccgcacgg cttaagcccg cgcactgaag gtgaatggag ttttaacgcc
     9781 gaagagacgg aaattgccga cagcgaagag aacaaaaaca ccgaccagca tcttgctacc
     9841 agccgctggc gcaagttcag ccgcgagtgg atccgcaccg cgaaatccga ttcgctggat
     9901 atctcctggc tgaaagataa agacagtatt gatgccgaca acctgccgga gccggatgta
     9961 ttagcggcag aagcgatggg tgaactggta caggcgctgt ctgaactgga tgcgctgatg
    10021 cgtgaactgg gggcgagcga tgaggcggat gcgcagcgcc agttgctgga agaagcattt
    10081 ggtggggtga aggaatgagt gctgggaaat tgccggaggg gtgggaacag attgaaatag
    10141 gcgacattgc tgatgtcatc tctggtggga caccaaaatc gggagtcgct gaaaattttg
    10201 caccttcggg tgagggagtc gcctggctta cgcctgccga tttgagcggt tataaagaga
    10261 aatatatctc acatggtgcc agagatttaa ctacactagg ctattcctct tgttcagcaa
    10321 aactaatgcc caaaggcact atacttttta gtagccgggc accgattgga tatgttgcta
    10381 ttgctgcaaa tgagattgcg actaaccagg gtttcaaaag ttttgctttt ccttcagaca
    10441 tttttcctga ttatgcatat tactttctcc gcaacatcag acatattgca gaggaaatgg
    10501 gcacaggaac aacttttaaa gaaatttctg gtagttctgc gaaaacatta ccatttgttc
    10561 ttgtaccgtt tgccgaacaa aaaatcatcg ctgaaaaact cgatacgctg ctggcacagg
    10621 tagacagcac caaagcacgt cttgagcaaa tcccacaaat cctgaaacgt tttcgtcagg
    10681 cggttctggg tgcagcggtg agaggaaaac taactgaaga ctggagagat aattcaagcc
    10741 tgtctgggtg gagagaaggc aaacttgggg agtttataaa aaaaccaagt tacggaacgt
    10801 catctaagtc taataaagaa ggactcattc ctgtattaag aatgggcaat cttcagggag
    10861 gaaagttgga ctggacggat ctagtctata cgtcagatac catagaaatt gaaaagtata
    10921 aacttgagta caatgatgta ttattcaatc gtacaaatag tcctgaactt gtcgggaaaa
    10981 cagccatata taaatcagag caaccagcta tttatgcagg ttatttgatt agggttcaat
    11041 gtttacctga tttaaaccct gactatctta actaccattt aaatagcatt ttggggagac
    11101 aatattgcta ttcagttaaa tctgatgggg tcagtcaatc aaatattaat gcacagaaat
    11161 taattgcata cccaataact gttccccccc ttcccgaaca agctgaaatc gttcgccgcg
    11221 tcgagcaact cttcgcctac gccgacacaa tagaaaaaca ggtcaacaac gccttagccc
    11281 gcgtcaacaa tctgacgcaa tccatcctgg caaaagcgtt ccgtggtgaa ctcaccgccc
    11341 agtggcgggc cgaaaacccg gaattaatca gcggtgaaaa cagcgccgcc gcgttgctgg
    11401 agaaaatcaa agctgaacgc gcagccagcg ggggtaaaaa agcctcgcgt aaaaagtcct
    11461 gatcattatt ttctggcgca cctttctggt gcgctattta ttatttttac gccaatcata
    11521 actcacacaa atatatttaa atcgttccag aaacttccca ttttattcta tttttagctg
    11581 gactttccct gtatttactg atgatatatc caggtattta gcgcgatgcg gatgtgcgcc
    11641 aacacacccg caccgctcat cacaatcact atttatggag aatagtaatc atgactgaca
    11701 cgcattctat tgcacaaccg ttcgaagcag aagtctcccc ggcaaataac cgtcaattaa
    11761 ccgtgagtta tgcgagtcgc tacccggatt acagccgtat tcccgccatc accctgaaag
    11821 gtcagtggct ggaagccgcc ggttttgcca ctggcacggc gatagatgtc aaagtgatgg
    11881 aaggctgtat tgtcctcacc gcccaacccc ccgccgccga agagagcgaa ctgatgcagt
    11941 cgcttcgcca ggtgtgcaag ctgtcggcgc gtaaacaaag gcaggtgcag gagtttattg
    12001 gggtgattgc gggtaaacag aaagtcgcct gatacattga cccggtagcg ccagtgctac
    12061 cgggtattat tcgccacgtt ataccaacgc gctctccatc tgtttttcaa atgcctgacg
    12121 aattaatccc ataatgtaag gaagttgtgc gaggtcactg aaaccaattt ccacatcacc
    12181 attgccccat cgcccaacat ttgtcacatc tttggcaata cctttcggat cgaccagttc
    12241 atgaaactgc atattaagcg acaaccgcag tcggcttttt tgcggtacaa catcaacaaa
    12301 attcgtttca gctttaaacg caatatagag ctttaaaact tcctgcgtga tccctgcgtc
    12361 caggcgcata acttcatcgc gaagatgatc aaataacacc cggctatgcg aaccatcagc
    12421 aagaaaagga taatcactca ggctgtaact ggttttgttc tccggctgcg cccgataaac
    12481 agctaaaacc tcttcaggaa gagcaggcgc ttgccagacc tgaaccgcga gatcggccag
    12541 acgatctgca cgggcgcgaa tggctgcttc atcccatttt tcgcactgtc caagaccgat
    12601 attcaaatag agcgggctat gtttaaagcc gtcttcaata tcgcgttttt ccgcgaaagg
    12661 tctgtcactg tagcgggagt tatagcgcgt taatgtgaga ttccccaacg tatgcaacag
    12721 ttctttatga atgcgttgcc agtcacttcc cagctcttca cgccatttag ccgacaaatt
    12781 ttcgttctgc ggcataatat gctcaatcgt aaactcttcc acgcgctctc tgcgtttatc
    12841 gttttccagt cgtcgtaacc agtagctgcg actacggaaa ttatagagat cacgaacttt
    12901 taattcccgt ttaaattcat catcgttggg gaaacgacga tatgaaggca gattcagaaa
    12961 atgtacctga atactttcca gatatttttc tttattaatg accttataaa aagtagcaaa
    13021 cgtcttgtta agagaatttg tcggaatcgc acatactgca cggcggaaaa cataagattc
    13081 aattaaacga attatgctca ggaaatcttc gtgagacaaa acgccatttt tatagtcatg
    13141 ataaagcgcc agtaagaaag gatacgccac atcgaccttt aactcgcgca aatcctgaaa
    13201 agccgtagca agcgatttgt cactttcttt tcccaatgcc attgcacagt aatactccgc
    13261 gtagatgtga atatcttcaa ccagccgatc tacgcctttt tcagcaacac tctggctgcg
    13321 ggcatggagt ttaaatgcct catagacttc atctgtccga gggatctccc ccgtttttac
    13381 cgtcagataa tgacgcataa aactgtcaaa atattcgctg taaccttgct gaccaaacgc
    13441 gacttccatt ggacgccagt gatcttcata caaccgggtt tgatgctctg gttcgaggcc
    13501 catcagaata aaattgcgca ccagatcggc ctgacttaat gccttaccgg tggagttcat
    13561 actttcaaaa atcagttgcg gattatcctg accacgatta agcgccacat cgacaattaa
    13621 taactttgct aacccacgac ataaggggat caagtcatca ccgagtttgg taatttgttc
    13681 atcaaagaaa gtaaagtttt ccattattcg atgggaatag ttttctggca ttggtctttg
    13741 ttttattaac gccagcaaac tgtctttatc agtctcggtc agcagtaatt taaatccttt
    13801 ctcgccggac tcataaggat tgagtaaata ataattacgc aatttcattg ctgaaaagcc
    13861 atcaaaaact tcgtcttcgc caagatgacg cgataaagcc tcaatcagca acattgcggt
    13921 cgtcagcctt tgttgaccat caatgaccag taacggagaa ataccagaaa cctgatacaa
    13981 cccctgctca atataaacaa ccgaaccgat aaaatgcgct gatatatcgt cacgctttcc
    14041 ggcacgaatg atgtcgtccc aaagttgccg acattattgt tctgtccatg aataagtccg
    14101 ttgataaatg gtaataacga actgctgcga acgttttaaa aaatcgagta ggcgggcttc
    14161 tgtagctttc attctgacgt ctttccgtga agcgatgatt tcgcaataat aaccccttat
    14221 ctgacgaggt gacatataaa tcccatcgtt tcgtgaagga ctctctccat tacaccacat
    14281 cctccctgca taacccaata tcctttaacc gttcatctct cacctgctgt aacacgcacc
    14341 gggtctgcat ttgctgccac cagcagcgtt aatgtaccgt tcacataaat ggcggcggca
    14401 acgttggtgc aggaaggtca ctatcatcgc cacgtaaagt gtgcttcgtt catgccggat
    14461 gcggcgtgaa cgccttatcc ggcctacaaa ccgcgcaaat tcaatagatt gcagagataa
    14521 tgtaggtctg ataagcgtag cgcatcaggc agctttgcgt ttgtcatccg tctctgatta
    14581 gctcttgagc catgaataag gctcacgctc aggcaagggc tgctctaatg cctgtatttg
    14641 ctctgccgaa agtccggtaa tcttttgtat ccattcagga tctaccccat tctgcaaaaa
    14701 caatcgaaga atgcgctgtt ccccttttat atatccatca ttatgaattc gctctgcaat
    14761 cgtcattatt cgctcccttt gttgtggcag tcgacgggta atttcactga taaactcatt
    14821 aaaacgcgct tcatcaccag tcagtaaaat gtaatttaac agcgcagtta tctggctgtc
    14881 attagcacac tctgtaacca gtaatactac cagttggtca ataagcccca tcagatcgcg
    14941 ctggcgaata tgcttttgga tcaactccaa cagggcgact ctgcgatgct gcacaatctc
    15001 gtcgtctggc acgacagtga tatccaccag cgggaacgcg gcggtataaa gcttccgtgc
    15061 ggtagttggg tcggcaaatt cgtccagcca gcacaaagac cagggataag ggctacggct
    15121 accgtgataa aacagcatcg ggatgaccag tggtagctgc cggcgtttat catgttcgat
    15181 atggcgctgc atcaccgcca tggaatatcg catcagacga aaggccatat gaatatcctc
    15241 acggctctga tgttcaatca ctacataaat atagccatcg ccttcacggg tctttaccga
    15301 ccacagaata tcggaatgta gcgcccgcaa tttttcatcg acaaagctgg cggattccag
    15361 ttttaagcta tcgagatcgc acagttcacg taaatctttg ggtaagtgga tctccataaa
    15421 atcccgcgcg gtgtcagagt gtgtgagaaa ggatttaaat aatgcgtcat gcggcgtgct
    15481 ggtcgtaaag tttgtcatgg cgatccgtca cctgtagata agatggccca actttagcga
    15541 ctaacaaagc cattcacacc tggctatttc ctattccccg aggcggtttc caggctattt
    15601 ttattcttct gcaaacgata catttttatg gaagatatct cgctttttgc gttttcttac
    15661 cgtcaggctc tattcttacg atatgaataa actcattgaa ctcagacgcg ccaaaatgtt
    15721 ggcgctctct ttactgctta tcgccgctgc tacctttgtc gttacgctgt ttttgccgcc
    15781 caatttttgg gtgagcggtg tgaaggcgat tgctgaagcg gcgatggtcg gcgcgctggc
    15841 ggactggttt gcggtggtgg cgctgtttcg ccgcgtgccg attccgatca tttctcgtca
    15901 tacggcgatt atcccgcgta ataaagaccg gattggcgaa aatctcggcc agttcgtgca
    15961 ggaaaaattt ctcgataccc aatcgctggt ggcattgatt cgacgtcacg aaccggcgtt
    16021 gttgattggc aactggttta gtcagccgga gaacgcccgc cgcgttggtc agcatctgtt
    16081 acagatcatg agcggttttc tcgaactgac cgatgatgcg cgtattcagc gcctgcttaa
    16141 gcgcgcggtc caccgggcga ttgataaagt cgatctttct ggcaccagtg cgttgatgct
    16201 ggagagtatg accaaaaacg atcgtcatca ggtgctgctg gatacgctga tcgcacagtt
    16261 gatcgccctt ctccagcgcg ataaatcacg caagtttatt gcccagcaaa ttgttcgctg
    16321 gctggagagc gagcatccac tgaaagccaa aattttgccc actgaatggc tgggcgaaca
    16381 tagcgcggag ttggtttctg acgcggtgaa ttctttgctt gatgatatca gccgcgatcg
    16441 tgcgcatcag atccgccatg cgtttgatca cgccaccttt gccctgatcg acaagctgaa
    16501 aaacgatccg gaaatggcag cgcgagccga tgccgtaaaa agctatctga aagaagatga
    16561 ggcctttaac cgctatctca gtgaattgtg gggggattta cgggaatggc tgaaagcgga
    16621 tatcaacagt gaagattctc gtgtgaaaga acgtatcgcg cgggcggggc aatggtttgg
    16681 cgaaacgtta attgccgatg atgccttgcg ggcgtcgtta aatggtcatc tcgaacaagc
    16741 cgcacaccgc gttgcgcctg agttttccgc attcctgact cgccatatca gcgacacggt
    16801 aaaaagctgg gacgcacggg atatgtcgcg gcaaatcgag ttaaatatcg gcaaagatct
    16861 ccagtttatc cgtgtcaacg gtacgctggt tggcggttgt attggactta ttttgtattt
    16921 gctgtcgcag ctcccggcct tgttccccct cggcaattta tagaaatcca tgaaaaacta
    16981 agtggctaac cgcgagagag atcaaataaa ccacactaaa agaatggtaa taatgtggcc
    17041 tcttttcatt atctcccgtg gtacggggaa ggaaaatcat gtcacttgtc accgatctac
    17101 ccgccatttt cgatcagttc tctgaagctc gccagaaagg ctttctcacc gtcatggatc
    17161 tcaaggagcg cggcattccg ctggttggca cctactgcac ctttatgccg caagagatcc
    17221 cgatggcagc cggtgcggtt gtggtttcgc tatgttccac ctctgatgaa accattgaag
    17281 aagccgagaa agatctgccg cgcaacctct gcccgctgat taaaagcagc tacggcttcg
    17341 gcaaaaccga taaatgcccc tacttctact tttctgatct ggtggtcggt gaaaccacct
    17401 gcgacggcaa aaagaaaatg tatgaataca tggcggagtt taagcccgtt catgtgatgc
    17461 aactgccaaa cagcgttaag gacgatgcct cgcgtgcatt atggaaagcc gagatgctgc
    17521 gcttgcaaaa aacggtagaa gaacgttttg ggcacgagat tagcgaagat gctctgcgcg
    17581 atgccattgc gctgaaaaac cgcgaacgtc gcgcactggc caacttttat catttggggc
    17641 agttaaatcc tccggcgctt agcggcagcg acattctgaa agtagtctac ggcgcaacct
    17701 tccggttcga taaagaggcg ttgatcaatg aactggatgc aatgaccgcc cacgttcgtc
    17761 agcagtggga agaaggccag cgactggacc cgcgcccacg tattttaatc accggctgcc
    17821 caattggcgg cgcagcagag aaagtggtgc gcgcgattga agagaatggc ggctgggttg
    17881 tcggttatga aaactgcacc ggggcgaaag ccaccgagca atgcgtggcg gaaacgggtg
    17941 atgtgtacga cgcgctggcg gataaatatc tggcgattgg ctgctcctgt gtttcgccga
    18001 acgatcagcg cctgcaaatg ctcagccaga tggttgaaga atatcaggtc gatggcgtag
    18061 ttgatgtgat tttgcaggcg tgccatacct acgcggtgga atcgctggcg attaaacgtc
    18121 atgtgcgcca gcagcacaac attccttata tcgctattga aacagactac tccacctcgg
    18181 atgtcgggca gctcagcacc cgtgtcgcgg cctttattga gatgctgtaa ggagtggcag
    18241 tgacatattc gattggcatt gattccggct caaccaccac caaagggatc ttactggctg
    18301 acggcgtgat tacgcgccgt ttcctcgttc caacaccctt tcgcccggca acagcaatta
    18361 ctgaagcctg ggaaactctg cgcgaagggt tagagacaac accgtttctg acgctcaccg
    18421 gctacggacg gcaactggtg gattttgccg ataaacaagt gacggagatc tcctgtcacg
    18481 ggctgggcgc acggtttctt gagccagcaa cgcgcgcggt aatcgacatc ggtggtcagg
    18541 acagcaaagt gattcagctt gatgatgacg gtaacctgtg cgatttcctg atgaatgaca
    18601 aatgcgcggc gggcaccggg cgtttcctgg aggtgatctc gcgcacgctt ggcaccagcg
    18661 tcgaacagct cgacagcatt accgaaaatg tcacgccgca cgccatcacg agtatgtgca
    18721 cagtgtttgc tgaatcagaa gtgatcagcc tgcgctctgc gggcgtagcg ccagaagcga
    18781 ttctcgccgg agtgattaac gcgatggcgc ggcggagtgc caatttcatt gctcgtctct
    18841 cctgtgaagc gccgattctg tttactggtg gcgttagtca ttgccagacg tttgcccgga
    18901 tgctggaatc tcacctggga atgccggtaa atactcaccc tgatgcgcaa ttcgctggtg
    18961 caattggcgc ggcggtgatc ggtcagcggc agagaaaacg cgcatgaaag agtatctgtt
    19021 tttatttcac tccacggtcg gcgtcataca aacccgcaag gcgttgcagg cagcgggcat
    19081 gacttttcgc gtcagcgata ttccccgtga tttacgcggc ggctgcgggt tatgcatttg
    19141 gctgacctgt ccgcctggcg aggaaattca atgggtgatc cctgggcata cggaatcagt
    19201 ttattgtcag caggatggtg gctggcgctg cattgcacat tacgggattt ctccgcgtta
    19261 aggattcatt aagatcgcta cgtatacttc tcgatatctt attgatttgc cttaaaggga
    19321 ttaccgtgac aaaaagtatt tcgttaagta aacgcatctt cgttattgtg attctttttg
    19381 tcattgtggc agtgtgtact ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag
    19441 catcatttca gaattaccac gccaccattg acggcaaaga aattgcaggc ataacgaata
    19501 atatctcttc gctgacctgg tcggcacaaa gcaatactct ttttagcact atcaacaaac
    19561 ctgccgccat tgttgaaatg accaccaatg gcgatttgat ccgcacaatt cctctggatt
    19621 ttgttaagga tctcgagacg atcgaataca ttggcgataa tcagtttgtc attagcgatg
    19681 agcgcgatta tgccatttat gtgatttcac tgaccccaaa ctcggaagta aaaatcctca
    19741 aaaaaataaa aatcccgctg caggaatccc caaccaattg cggttttgaa ggattggcct
    19801 attctcgcca ggagcacact ttctggtttt ttaaagagaa aaacccgatt gaagtctaca
    19861 aagttaacgg gctgttaagt agcaatgaat tgcatatcag caaagacaag gcactacaac
    19921 ggcaatttac tttagatgat gtgtccggcg cagaatttaa ccaacaaaaa aatacgcttc
    19981 tggtgctgtc tcatgaatct cgcgcactcc aggaagtaac gctggttggc gaggttatcg
    20041 gtgaaatgtc actaaccaaa ggcagtcgag gtttgtcgca taacatcaaa caggccgaag
    20101 gggttgcgat ggatgcctct ggtaatattt atatcgttag cgaaccaaat cgtttttatc
    20161 gctttacccc tcaatcatcg cattaacagg ctggcatgtt tcaccttttt gttattaact
    20221 tgttaacaac aaaaatgaca aacaaggtta aacatgcctt cgccctcgca tcccgtagaa
    20281 cgcttttctt tcagcaccgc gcttttcggg atgctggttc tgaccttagg tatgggttta
    20341 ggccgcttcc tttatacgcc tatgttgccc gtcatgatgg cggaaggatc gttttcattt
    20401 agccagctct cgtggattgc cagcggcaac tatgcggggt atctggctgg cagtctgcta
    20461 ttttcgtttg gcgcatttca ccagccatcg cgcctgcgcc catttctgtt ggcttctgcc
    20521 ctggcgagcg ggttgttgat cctcgcaatg gcatggttgc tgccatttat tctggtgtta
    20581 ttgattcgcg tcctggcggg tgtcgccagc gccggaatgc tgatttttgg ttcgacgctg
    20641 attatgcagc acacgcgcca tccttttgtg ctggcagctt tgttttctgg cgttggcatt
    20701 ggcatcgcac tgggcaatga atatgttctg gcaggcctgc attttgacct ctcctcgcaa
    20761 acgttatggc aaggcgccgg ggcgctttct ggcatgatgc tgattgcact tacgctttta
    20821 atgccctcga aaaaacacgc catcacacca atgccattgg caaaaacgga gcaacagata
    20881 atgagctggt ggttactggc tattctgtac ggcctggcgg gatttggtta tatcatcgtc
    20941 gcgacttacc tgccgctcat ggcgaaagat gcaggctcac cattgttaac cgcccatctc
    21001 tggacgttag tcggcttatc aattgtgcct ggttgctttg gctggctatg ggcagcaaaa
    21061 cgttgggggg ctttgccctg cctgacggcg aatttgctgg tgcaggctat tagtgtgttg
    21121 cttactctcg ccagcgactc gcctctcttg cttatcatca gcagtcttgg ttttggcggt
    21181 acctttatgg gcacgacatc tctggtgatg actatcgccc gccaactgag cgtaccggga
    21241 aatctcaatc ttttaggctt tgtgacactc atttacggca ttgggcaaat cctcggcccg
    21301 gcgctaacca gtatgcttgg caatggaaca tcggcgcttg ccagcgccac tctctgcggc
    21361 gcggcggcgt tgtttattgc tgcgcttatc agcacagcgc aattatttaa gctgcaagtg
    21421 gttacttcat gaggagtgtt tcccttacca ctcaagaaac tcaaccgcaa ccgttgatgt
    21481 cagccagacc ccgttttccg cttgccaaaa agggatgcct cgtttcgcca tttcctgtgc
    21541 tttgacggtt aaaataactg gcgatccgtg tcttgctcct actttgcgtg ccgttgcttc
    21601 atcagcagag agatgcacgt aatggcgctc ccctgcaatc agcccttgtt ttttatctcg
    21661 tcaagaaaac ggcttgccgt accgtggtag agaaattgtg gcggtgtttt ttcgatgaag
    21721 ccaatggcga cctgcgaagt caaatgcccc tgaacggcgc gaatacacgt accatcactg
    21781 gaataactaa agcgcttttt atcgctggtg gcgacaacga tatccagtaa tgcgcgggtt
    21841 aaccgcttgc ccgctttttg ggcacagagt atgagcttat cgatatccgc ccatccttca
    21901 cggtccagca cgatgccaat cgcctctggt ttatgtcgga ggacaaaact taaaaacttg
    21961 ctcgtctctg ccagctcttt gtcgttgtat tttgccatcg cgttttcgtc cctgaatttt
    22021 ctgtttttat ttactgatgt cattgtcact gggtttatga attttctgca actcaatcct
    22081 gcgcagaacc tttgccacac agcgtcattt ttccgctatg caaaaaacgc atagcaagaa
    22141 acatacttgc attttcccca tacccacatg gctgataagg tttttcagct ccaggaattt
    22201 tcctggctca agcaacacac atacacaaaa aaacaatggg gattgttatg acacaacaag
    22261 gggatgctgt agctggggag ctggcgacag aaaaagtggg cattaaaggg tatctggcct
    22321 tttttctcac cataatcttt ttttccggtg ttttttccgg aacagacagc tggtggcgcg
    22381 tttttgattt ttccgttctg aacggatcgt ttgggcaact gcctggtgcg aacggagcca
    22441 ccacctcatt tcgcggcgcg ggtggcgcgg gggctaaaga tggctttctc tttgcgctgg
    22501 agctggcacc gtcagtcatt ctctctctgg ggattatttc aattaccgat ggtcttggtg
    22561 gattacgcgc cgcgcagcag ttaatgacac ctgtgctaaa accgttgttg ggcattccag
    22621 ggatctgctc gctggcgctg atcgctaacc tgcaaaacac cgatgccgcc gccggtatga
    22681 ccaaagagct ggcgcaggaa ggtgaaatta ccgaacgcga taaagtgatt ttcgccgcat
    22741 atcaaaccag cggtagcgcc atcatcacca actacttctc ttccggcgtc gcggtgtttg
    22801 cctttctggg cacgtccgta atcgtacctt tagccgtcat cctggtgttt aaatttgtcg
    22861 gagccaacat tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca acgcaaggag
    22921 cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat atgtttatcg atggcgcgcg
    22981 ccgtggcttt actatcgcca cgaccaacct gctgccaaac gtggtgatgg cgtttgtcat
    23041 cattcaggcg ctgaaaatta ccggcctgct cgactgggtg gggcatatct gtgaacccgt
    23101 aatggcgctg tggggattac ccggtgaagc ggcaaccgtc cttttagccg cattaatgag
    23161 tatgggtggc gcagtcggtg ttgccgccag tctggcaacc gctggcgcat taaccggtca
    23221 cgatgtcacc gttttactgc ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg
    23281 tcgctgcctg gggacagccg aagtgaatgc caaatattac ccgcatatca ttacggtctg
    23341 cgtgattaac gcattgctgt cgatctgggt tatgcagctt attgtttaac aaggagttac
    23401 catgattgat tataccgcag ccggttttac cctgctgcag ggagcgcatt tgtatgcgcc
    23461 ggaagatcgg ggaatttgcg atgtcctcgt cgctaacggc aaaattatcg ccgttgccag
    23521 caatatccct tctgacattg taccgaactg cacggttgtc gatctcagtg ggcagatcct
    23581 ctgcccaggt tttattgatc aacacgtcca tttgattggc ggtggcggcg aagcaggtcc
    23641 cacgacgcgc acgccggaag tggcgctaag tcgcctgacg gaagcgggcg tcacgtcagt
    23701 ggttggtctg ctgggcaccg actctatctc tcgccacccg gaatccctgc tcgccaagac
    23761 ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg accggcgctt atcatgtccc
    23821 ttcccgcacc attacgggtt ccgtggaaaa agacgtggcg attatcgatc gtgtgattgg
    23881 cgtgaaatgc gccatctctg atcaccgttc tgccgcaccg gacgtttatc acctggccaa
    23941 tatggcggca gaatcccgcg ttggcggttt gctcggcggt aaacctggcg tcaccgtgtt
    24001 ccacatgggc gacagtaaaa aggcgttaca gcctatttat gacctgctgg aaaactgcga
    24061 tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc aacgtaccgt tgtttgagca
    24121 ggcgctggag ttcacgcgca aaggcggcac catcgatatc accagcagca ttgacgaacc
    24181 ggtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg ggtattccgc tggcacgcgt
    24241 caccctcagc tccgacggca acggtagcca gccgttcttc gatgacgaag ggaatttaac
    24301 ccatatcggt gttgccggtt ttgaaacgtt gctggaaacc gtgcaggtgc tggtcaaaga
    24361 ctatgatttc agtatcagcg atgccctgcg cccgctcacc agtagcgtag ccggtttcct
    24421 taacctgacc gggaaaggcg aaattctgcc aggcaatgat gctgacttgc tggtcatgac
    24481 gccagaactg cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa
    24541 agcctgcgtg aaaggaacgt ttgaaacggc ttaaacggtt gtcagccgcc cgaatggacg
    24601 tagaatgccc gcaagggcgg ctgacagagt aaaacgtaat ggatgactgt ggtgcgattt
    24661 tgcataatat tgaaaccaaa tggctttatg attttctgac cctggaaaaa tgccgcaatt
    24721 tttcccaggc ggcagtcagt cgcaacgtct cgcaaccggc attcagccgc cgcatccgtg
    24781 cgctggaaca ggcgattggt gttgaattgt ttaaccgcca ggtaacgccg ctgcaactct
    24841 cagaacaagg gaaaatcttc cattcgcaga tccgccatct gttgcaacag ttagagagca
    24901 atctggcaga gctgcgtggc ggcagcgatt acgcgcaacg aaaaatcaag atagccgctg
    24961 cacactctct ttccctcggg ctgttaccgt ccattatcag ccagatgccg ccgctcttta
    25021 cctgggcaat tgaagctatt gatgtcgatg aagcggtcga taaactgcgt gaagggcaaa
    25081 gtgactgtat tttttccttt cacgacgaag atttgctgga agcgccgttt gaccacattc
    25141 gcttatttga atctcaattg ttccctgtct gcgccagtga cgaacacgga gaagcacttt
    25201 ttaacctcgc gcagccacac tttccgttac tgaattacag ccgcaactcc tacatggggc
    25261 gattgattaa tcgcaccctg acgcgccaca gtgagttaag tttcagcacc ttttttgtct
    25321 cttcgatgag cgagctttta aagcaggttg ccctcgacgg ctgtgggatt gcctggctgc
    25381 cggagtacgc catccaacaa gaaattcgca gcgggaagct cgttgtactg aatcgggacg
    25441 aactggtgat cccgattcag gcctatgcat accggatgaa tacccgcatg aatcccgttg
    25501 ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt gcttagctga cattctccag
    25561 cgtcgcactg cgccaaccgc cattatccca gaccagatca aacgttagcg gttcatcgtc
    25621 gtggtacagc attatctcaa ggctgatcgc cagagtgtgg cagtacgctt ctttcaaatt
    25681 aacctgcaac gccgcaatcc gtggctcatg aaataacagt gcagaacgca gctgacggca
    25741 ataccagctc tggagatgtt tgtcgctcgg gttatgcact tgccactcgg gcatgcctga
    25801 aacaagtaat gacggtaatg ccgaatgagg agaattaaaa agagtgagga gatcgctgca
    25861 taccgattta cgcagcgatg acgttttatt gcctgaaatt tcaggtaaaa cagcctgaag
    25921 tgattgtcgc atcattttgc gcactcctga cgtttagcaa catatgcggc ttgccctgaa
    25981 caccgggcac cactgtattg atgctcattt tccatagcca ttcccttttg ttcattacat
    26041 ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag tataaaacct tacttgccta
    26101 tgaatatcta ctgacttttc aggaaaaaag tcatgatttg ataaaaatca tcaaactcaa
    26161 aaaaaaaccg ctagtatatt ctgaatattc cgaatagtta cgtagatgaa aatacttgtc
    26221 accgctgcta cttaattccg gtgggaaatt atttatttcc gcataatcag ccatgtatta
    26281 tttgaaacaa ttagtttgta aataatataa attgataaca tatcaaatac atcaaaaata
    26341 ccattcacag gcattattaa tatagacctc aatatagcaa catcaaattc caattagtaa
    26401 taagatatgc ttttaaattg catgcaaata attttaaatg agcgtttttt gatactttag
    26461 agtgtttcct accctttaat atcaccgtgt agagttgata tcactacaac agaaacatgc
    26521 atgtcatttg tttacttctc tttttactac agaaaaattt atctattttt ctaaacatca
    26581 gtgaaatact cataaaaggt aactattatg tctatgccat taagcaatgc ccttcagagt
    26641 caaattatta cagatgctca cttccttcat cacccaatag tcgacagtga gtttacacgt
    26701 aaattaaaat atgcacgcat ggattcggaa aacatctatc ttccgcctct tactcgtggc
    26761 aataaccata attacgatgg aaaaagcgtt gtcgaaatta gaaaacttga tattagtaaa
    26821 gagccttggc catttaatta tgtgacagga gcatgcagag aatccgatgg tattacaaca
    26881 acaggacgta tgctctatcg gaacttgaaa atcacctcag ctttagacga aatatatggt
    26941 ggcatatgta aaaaggcgca tgctaccaca gagttagcag aaggtttacg gctaaattta
    27001 tttatgaaga gccctttcga tcctgttgag gactatacag ttcatgaaat aacattaggc
    27061 cctggttgca atgttcctgg ttatgccgga accacgatag gatatatttc aacccttcca
    27121 gcttctcagg caaaaagatg gaccaatgag cagccaagga ttgatatcta tattgatcag
    27181 ataatgacgg taacaggggt agcaaattct tccggattcg cccttgccgc attacttaat
    27241 gctaatattg agttgggtaa tgacccaata attggtatag aagcctatcc cggtaccgcg
    27301 gagatccatg caaaaatggg atataaagtt atcccgggtg atgaaaatgc accactcaaa
    27361 cgtatgactc tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg
    27421 aattatattg gtaaataact accaggaaga atgagtacta acattcaggt tatacagata
    27481 gctaaacaat accctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa
    27541 gcgcaaggaa cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc
    27601 ggccatgaat caaacagata gccatcaaaa taaatatcgt caacgttcga gaattccagc
    27661 agacgttgct taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct
    27721 tttttgatta acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg
    27781 ctgtcatcca gatggctgtg caactggatc cacattggat tgttttcccg ccactgccag
    27841 gactgacgga acagctccac cagcatgctg ttatgcgttg cttcggcaat agcgagatgg
    27901 aactgcatgt caccgctttc gctgctgccc ggcgcactgg aagccagttc acgctcttcc
    27961 agttgcaatg cctgacgcat tttgacgata tcttcgcggg tagcctgcaa agcggcaaac
    28021 tcggcgatgt tgctctccaa taactgccgc gcctgtaaca gctcaaaagg accggcatcg
    28081 ttgcagacgt tggcatccgg actgtctgtg ttctggctgc ctgagttatc aagaacatag
    28141 ataccggcac cccggcgtac ttccaccagc cctttgatct ccagcatgat cagcgcttca
    28201 cgtaccaccg tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt
    28261 tcgccaggat tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg
    28321 taaggtcttt gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat
    28381 caaatttttg ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg
    28441 cccccgttaa tctcaaacta taccattagc actggaagtt ggcacgtaca ggtgggcaaa
    28501 aaaacgaaaa ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc
    28561 agttagttac agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc
    28621 cgccgtcact gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct
    28681 caggcccagc agcgctttca cgcggtctgc gccctgatac tgcgcgttga tcttctggaa
    28741 ctccgccagc atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt
    28801 gtaacgcatc cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag
    28861 gtgcagacgc accgggtcca gcagacgctg cggtaacttc tggctgccgt ccatcgcaat
    28921 ctgccaggta cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata
    28981 ggcgttcagg tctgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag
    29041 ggcaaaggcc gctttgcgat aagccgggtt agtcacggtg tcggcaatgg tttcatagcc
    29101 gccgaggtaa ccgaggtacg ccagaaaaga gtggctgccg ttcagcatac gcagcttcat
    29161 catttcgaac ggcacaacgt ctgcaacgaa ctgtgcgccc actttatccc aatccgggcg
    29221 accattaacg aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca
    29281 cgggtcgtaa acacccagct ggtcagcaat ttcctgtatg gtttctggcg tcgccgccgg
    29341 aacgatgcgg tcaaccatgg tgcacgggaa ggtgacattt tcttcaatcc atgccgccag
    29401 ctgcgggtca cgcgcctgag ccagccccag taccgcgacc ttcgccacat gaccgttttc
    29461 acgcacgtta tcgcaggaca tcaccgtaaa cgctttcaac cctttttcac gacgcagacg
    29521 caacgcttcg acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg
    29581 cttgatcagc gggttattga gatccagttg accgcttgcc gcatcagcgc agtagccttt
    29641 ttccgtgacc gttagagaga caatcgccgt ttgcggacgc gccatcgcgt tgagaatacc
    29701 ttcgcagcca tcgatttccg gatgcagcgc ttctttcatc gaaccgataa ttttcagctc
    29761 ggtgctctct gcgccttttt ccgctacggt gtacagcagt tgctgttttt tcaggttttc
    29821 gatcagcacg cggtcgttgc ctggcatcag gttaacttcg cagatgcccc agtcgctgtc
    29881 ggtgctttcc agcagatgat gggtatacag cgcctggtgc gcgcggtgaa acgccccgca
    29941 accgagatgc acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg
    30001 cagattgctg tcaacaatag tagtcatcgt aaactccaga atccgtcttc aaagagtgac
    30061 ggcaaggaag aaggaaccgg cacggcgcgc agccatgccg gtggatatta acggctaaag
    30121 aaagcgcgct ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca
    30181 attgcggagt aacctgggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg
    30241 tccggacgca tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca
    30301 acaatcgctt tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg
    30361 aaggttttcg ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga
    30421 ccgaactgct tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg
    30481 cacatggtaa aaccgtttgc catgctgttt acggtatcaa ccatccactg catatcttca
    30541 atggtggaaa caatgcgcgg caggccgagg atcgggcgcg gcggatcgtc cgggtgaaca
    30601 gccatacgca cgccgacttc ttcagcaact ggaataatcg ctttcaggaa gacggcaaag
    30661 ttttcgcgca gcttcgcttt gtcgatatct ttgtacagct ccaggtgttt acggaactgg
    30721 tcgagggtgt agccttcttc cgcgcccgga agaccagcaa tgatgttacg ggtcagacgc
    30781 gctttgtctt catcgctcat agtggcgaag cgttcagctg cctgagcaat ttcttcttcg
    30841 gtgtaatccg cttccgcgcc tgggcgtttc aggatatgca tttcgaatgc agcgaattcg
    30901 atctggtcga agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg
    30961 gtccagtcga gcaccggcat gaagttgtag cacacggtgc gaatgccaca ctgcgccagg
    31021 ttgcgcagag tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg
    31081 atatcttcgt gaattggcac gctttctacg acagaccaca ccaggcctgc gtcttcaatg
    31141 atcgccttgc gtttgaggat ctcttcaacg gaccatactt cgccgttcgg gatatggtgc
    31201 agcgcggtaa ccacgccagt tgcgcccgcc tgacggacat cagctaaaga aaccggatcg
    31261 tttgggccgt accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc
    31321 cacagcaaag agacgcggaa ccctggagtt gtttaggtat ccggatgaac aaccagaccg
    31381 cagccgtggc gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt
    31441 caacccgaat tctgaaattg gttaaccaca tcacaagaat ttcactctca tggttgttgc
    31501 tgcaaattgc agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca
    31561 acaatggttg accaatttac ataacatatc gcccaaaata acaacggttc aaccaggtaa
    31621 ggtaaaacgt tatgcatgtg cttaacattc tctgggtggt attcggcatt ggtctgatgc
    31681 tggtactgaa tttgaagttc aaaatcaatt caatggtggc tttgttggtg gcggcgctgt
    31741 ccgtcgggat gctggcgggc atggatttga tgtcgctgct gcacactatg aaagcgggct
    31801 tcggcaacac gctgggggaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat
    31861 tgatggtcga ctccggcgcg gctcaccaga tagcgcatac gctgctggcg cgtctcggtc
    31921 tgcgctatgt acagctgtcg gtgattatca tcggcctgat ttttggtctg gcgatgttct
    31981 atgaagtggc ctttatcatg ttagcgccgc tggttattgt tattgccgcc gaagctaaaa
    32041 ttccgttcct gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt
    32101 tcccaccgca gccgggtccg gtggcgctgg tgaatgctta tggcgcggat atggggatgg
    32161 tttatatcta tggcgtactg gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc
    32221 cgaagttcct cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg
    32281 tagatatgaa taatctgccc tctttcggcg tttcgattct ggtgccgctg atcccggcga
    32341 tcattatgat ctctaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg
    32401 aagtggttaa ctttatcggt tcctcgccga ttgcaatgtt tattgcgatg gtggttgcat
    32461 tcgtactctt tggcaccgca cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa
    32521 gcgcggtgaa gagtattgca atggtgattc tgatcatcgg tgcgggtggc gtgctgaagc
    32581 agaccatcat cgacaccggc attggcgaca ccatcggcat gttgatgtcc cacggcaata
    32641 tctcgcccta catcatggca tggctgatca ctgtgctaat tcgtctggcg acgggtcagg
    32701 gtgtcgtttc ggcgatgacc gccgccggga ttatcagtgc tgcaatccta gatccagcaa
    32761 ctggtcagct ggttggcgtg aatccggcgc tgctggtact ggcgacggct gcgggttcca
    32821 acaccctcac ccacattaac gatgcatctt tctggttgtt caaaggttac tttgacctgt
    32881 cagtaaaaga cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc
    32941 tgattattgt gctgattatt agcatggtag cgtaaaagga caccagagcc tgccaatggc
    33001 aggctcagac tgatgacaaa gtctaaaaat gtgcccggac agtcccctcg cccctccggg
    33061 gagagggtta gggtgagggg aacagaccag caaagtgcgc tttgtttatg ccagatgcga
    33121 cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc aggtttttag cttcaggtaa
    33181 tattgcgtac cagcattagc aatgtcctgt gatttcttta ttgataaaca aaagtcacgc
    33241 caataatcga ttgcacattc cctgcagtca cctgccctcc ggtacgtgca taatttgccg
    33301 ttaatcccag actcaccgcc gaagtcccta ctgctcctaa cgataccgtg ttattcgctg
    33361 gaataatcgt accgttgcgc gtcaactgta cgccgacgcc ctgtgcaggt gaaaacgacg
    33421 cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt gccggagagg taatacccca
    33481 ggttttggct tttcgcacaa taaacggtaa gaggaattgg cactgaacca gggtagtccg
    33541 gcagagtaac ggtgacatca cgagcagaaa catcgcagcc gccagtaggc accaccacat
    33601 cattattggc gtaaatattc cacacaaact ggaaatcatc gctgttatag ttgttggtct
    33661 gtcgcaaaat aagcacggca attaatgagc cagctttaat cgccaccccg cccgcactgc
    33721 tcacaggcgt caaataaagc gccaccggcc acggcttatc cgttctcgaa ttataaacaa
    33781 cgcgcggcgt ttcgctggtg gtaggaaatg gatagctact gccactatat tttacggtcc
    33841 cggaaaaatt agataacacg ccgccataag ccgagcctcg ttgcagtgtg acatagtctg
    33901 taatggtttc cggataatcg ttatggcaaa agatttgcgt cgaaagatcc acgaccaggt
    33961 tttgccccac attcacgacg ggcgcaaggt ttacataaac attggcgctg ccaccgccaa
    34021 tagggatagc ggtaccattg gcggttttac aggcgaatga ccaggcattt accgaccagc
    34081 ccatcagcag tacagcaaac agggtaataa ctcgtttcat tacaatcatc tcttcgggtt
    34141 cagctgtagg tataggtgat gctaatcact gcctgaatgg ttccctgagt ggctccgcca
    34201 tttactgtca atgctctgac ctgtaacggg aagtgcgctg attgtgagga atcatccacc
    34261 tgaactgttt tggttgcgcc agtattcaat gtgttgccac tgtcatcctg tagctctaac
    34321 tggatgtttt gcgcggtccc ctggttttta taatatccgg tactgtcggc tgccccgctg
    34381 aagctggcag tgaccctcga cgttcccacc ggacaattag tcaactcaag cgcaacatca
    34441 tgccaggccg atgccgcccc ggcagacata agactgaaag aataaagatc gccgagatca
    34501 accgtggcat tggtggtgga aaccgtacac ggtttggcga cgaccttacc gttcaccgtg
    34561 atggtgacat cggctgcctg tatcgtcgca cttgcgagcg ccaatattgc cgccaataca
    34621 tacccacgtt tgcaccattt catgagcatc tccagttact gatattcaag agtgaaggta
    34681 gccgtggcat tgatatgccc cgcagtgaca ggcacctgtg tcgccattag ccgggcgtaa
    34741 aaattcagcg tatttggttt acccggcgtc agggtcgtcc acgaaagcgc ggacgatgga
    34801 gcattaaggg gtatttgatt ttgcttctca ttcagaagct gtattcccag tcccgaagcc
    34861 gctgacaccg tattttcaag tgcaagcagg ttggcattgt ggctatctgc aacgccagta
    34921 aacccaacct ttacggcaga aacggcatta ccacagggtg acagcaaaat acgaaatgga
    34981 acaacaggag tcgtcgcgcc aatgttgtta aattgcttcg ccgcgttttc catcagatca
    35041 acagtaaaat tggttgattc agcggccaca ctacagccgt tatccctgac atagccgcgg
    35101 atagtaatcg tgctatccgc agccaaagcg tgactcaccg ccagccacaa aaaagcgcac
    35161 agaagataaa aaggtttgtt tctcatcacg cccccttaac gacattcagc tgatagctgg
    35221 gttaataact gctgctgact ctctggtggc agttgataat tggcgacaca gtgagcattt
    35281 tcctcttctc cccatttcac ctgaactttt cccgctaaag gcattccgct gaggtaaacc
    35341 tgaccattat ccgcaacaat gccgctactc tggctactct ctgatgtcac catcgcccca
    35401 aacggcagcg gcttattatt gtgggtcagc gtcatgagca gttttatccc aacgcgcgct
    35461 ttaaactctg ctcgcacgat cgccccacga gtgggaacaa cgttagcaac cgcgttatct
    35521 aaatcgacgt tatcagccag ggtattggta tccagcgcca ctctattttc ccgatattca
    35581 gtggcataag gcagcacggc ataaccacgc cagtcggtac gcacccccgt ctggttttcg
    35641 acttttgcat cttttgcgcc aggcgcttta acaagcacca ccgtatcgtt taacggctgc
    35701 cccagcgtta cgccattggc atgagccagt accccaccgc tgactccgta atagagctgc
    35761 ttaatatcat cgctatggct gtaaccgata ttggcattgc cgtaaccacc gcgataattc
    35821 agcgtggcgt agcctgtact tccgctattt ccatcgcctc ccccggcata gccggtttgc
    35881 acgctatagc tgaggttgtt gtcttccagc aacgtaccgt atacaccagc cagattggtc
    35941 atccgaccgt tgagatcgtg tgacatgctg tagctggcac tggcatgtcg ccactgagat
    36001 ttactgtcag aacgcagcca gtggctgaaa ggaatattga cgttaagcgc taacatctga
    36061 tcccgtcctt tttgccaggc gtttttcgtc aggctatagc tgagcgtcca gttgatatct
    36121 tcgaacgcag tatttaatcc agcctggaat tgctcatcga cattactcgt tccccaataa
    36181 gtttgatggc taccactcaa atacagtgtt gatgtgcgcc cgagttgctg agtaacggtg
    36241 agttgtaatt tcccgcgttt gttataagcg aggttgtaat agtcggtgaa tttcggctta
    36301 acctgaataa ctccgtcctg tgtttcgatg ttgtagccat tcattcgact gtatgttgta
    36361 tcagcgaaat taaaatatcc gctggtcgaa taacggtaac ccactaactg aatattcgtg
    36421 cctgattcat tgagcgattt gttatagaga aaacgcaccg attgtccgtc atgctgactg
    36481 tcatcgggaa gtgtggaatt agcctgcgtc atatccacag acagagcgcc cagtgccccc
    36541 atgtttttcc cgataccgaa attaaaagca cgataacgat ccgccagttg cgttccacca
    36601 tatattgtcc agccagccgg aaggccgtgg agtaatgtac tctggaaaaa gcggggtttt
    36661 tcctgctgcg catttccact acggtattct cctgccgtaa tggaataacg agtatgccct
    36721 tcacgttgca aaagcgggac tgacgaatag ggtacggtaa aaatctgcgt gctgccgtca
    36781 gcctctttga tcgttacctg caagtcacca ctattacctg cggcatagat atcgttgatg
    36841 gtaaaaggcc ccggtggcac cgtactatta taaatgtcat acccattttg tttaatagtg
    36901 acctgtgcag taccacgagc aataccgtgg atcaccgggg caaatcctct ttgactatcg
    36961 ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa agttaatacc atcgaaaata
    37021 tcgccctgag tataaccatc acccagcgtc agccgggaac gtaacggtat tatgtctcgc
    37081 tcaagccagg tattgatatg ctgccattta tttttgctac ctgatgatct gtcgctactg
    37141 ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac caatatttaa cccactctgt
    37201 aggtttaaat atgcataatg gctgttaccc ccaatccgat tctgtacact atttccgctg
    37261 aaattataat tgagcaatcc ggcattaata ccgggatccc ataactcagg aggaatataa
    37321 ccacgcgcgc gattactcat aaatgcctga gggatcgtca ggttcagtcg ctgctgacca
    37381 acatccagat gcgcagtagc gtcctggacc attgtggtta atggcacaca ggcatcatcc
    37441 gccagcagat tcataccggc gacagaagcc gtattcagcc ccatactggc gagttgcgcg
    37501 cgtgtcaggc agggaacaat cccttgttca ctgtcgcccg tattaaatgt gacatcacgc
    37561 gttgccatat aaccattatt caaatagata tcgacgcgat acgtccctgg cggtaattct
    37621 tgcccatttt caaaacgcga taaatcggcc acagcctggg gatcatccgc taaaaagcgc
    37681 ggattaaaat agaggtcggc agatgacaaa ggtgcctgtg cggcaaaagc acaggcgaca
    37741 acgagtcgga caaaaaaacc agccaaacga tgcttacgaa tatgcaagca ttgtgtgttt
    37801 cgctggtaaa gtcttaaatt cagatatgac attattcctc cggcttaaaa tcacccggca
    37861 gtcaattctt tttattcagg cgaaaaattc cccctgcgtt attccattac gcccgtcatt
    37921 ttgggggtaa gtgcgccata atcatttatt gttcggtaag taatattgct tcctgcatca
    37981 gaaggcaatt taaccgtgct ttcgcccatt ggaggcacca atgcattttc aagaacccgg
    38041 gttccggcat tcaactctgt taccgtcagg taatagggtg tcgggttaat cagcgtcaga
    38101 gaattcgcgc tacgacgaaa tcttaatttt tctgcggcct gatcgggtgg caacgctaat
    38161 ttagccgggc gatagtacag tttaatgcgg ctgataattg cgagctgtag cgtattctca
    38221 gtcaattttg atttatccat tgacggaatc gctttaacgt tcatccagaa taaactttcc
    38281 cggtcctgtg gcaattggtt atttgttgca tcaagaatac gtaaggtatt ctcttttttt
    38341 cccttcatcg caaacagagg aggcgtcacg ataaaacgac catcctttac accatcggca
    38401 ttttccaccc atgattgaat taaataggta ctattttcat cattatttgt cacggcaagt
    38461 tgcacttgtt tttgccctgc cggataaatt acgcgagtcg cacctaaggc cactcccgct
    38521 tcagcgcgtc cggcaaccat cattgccatg aacatcagga tacctgccag caagcagaat
    38581 gttatttcct gcgatttcct tacattgacg tttttattac tcactgtcct gttcctgtta
    38641 tcacattatc tgctgaacaa ttactgatag gttaaagaga accaggcctg ggcattggcg
    38701 atgccgccag taacccgacg cccggtagca cgatatttgg cgatgaaatg tagcgaagta
    38761 gagcctgaat aaagccgttt ccagtttgct ggaggacgat taatcggtac gaggtttcct
    38821 tcatcatcaa acaacgctac gccaatattg gtggctatcc ctggcccctc tcccacggaa
    38881 agcacatccg gatttttacc atccgcgaca ccgtgaaacg ccacacctac acgttcactc
    38941 accaccgtgc tacattcccg taaatgaata acaaaaggca ccggtgcgct atcttcccca
    39001 accgcatgaa accggttact gctgatttgc cccatattga ccgtcatttg tttatcaccg
    39061 gcttcaatcc ggcaagtttc cgcaataatg acgccctgaa attgcatatt tccgccgggc
    39121 aacgtggtat tccatttatt tccggccaga gcaaacattg gcagcaacga cgctaataga
    39181 aataatctct tccttttcat cgttattttt atcgcacaag ggtgggcatc cctgcccgta
    39241 atgacgtccc tgaacctggg taggttattg atactgaacc ttgaaggtcg catccgcatt
    39301 agcagcaccc ggggttgcgg ccccggttgc aaaataacgc gcctggaacg gaatggtatt
    39361 ggttccgtta ttcagggttg tttctgaact aaatgtcgca ccatccagcg tcagcgcagc
    39421 acccgttctg tccaggatct gcacaccaac gtttgttgcg ctacccgcag ctgaactctg
    39481 cagagccaga acgttggtat gacccacatc aatcgccgta cctaaaaagg caacagcggc
    39541 tttagatgca acattggtat cgcaatcatt cagctgaatg ttaaaaccgg cagcagaact
    39601 ggttgctcct tcctgtgcca gcgatgcggt acgaacctgt cctaactgaa cggtttgatc
    39661 aacagagcct gcatcaactg cgcaagcggc gttaacaact tcccctttaa agtgaacggt
    39721 cccaccatta accgtcgtgg cagcggccag agccgctgta gaactgaggg acagagccga
    39781 cagaacaacg attgccagag ttttaatttt catgctgctt tcctttcaaa aaactatttc
    39841 taaatcgaca tgggcagtcg ttctgtacac tttgttttgt caacgagttt gactgccaac
    39901 actgcacagt tttcccccaa aagatgaaac atttggggcc attttgactc atagaggaaa
    39961 gcatcgcgga caaacttttt cagtttattt gttggcttaa tattctattg ttatctttat
    40021 ttatagatgt ttatattgca tgaggtggtt tttggagaga agaatgagga agatgcgtcg
    40081 agccacagaa acgttagctt tacatatagc ggaggtgatg tgaaattaat ttacaataga
    40141 aataatttac atatcaaaca gttagatgct ttttgtcgtt tttaatattt ttatgcttga
    40201 gaaaaaatac gtaacttatt tatgata
//
LOCUS       DAYPHN010000024        39573 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000024 DAYPHN010000000
VERSION     DAYPHN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.024"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..289
                     /locus_tag="ACG0BW_001263"
     CDS             <1..289
                     /locus_tag="ACG0BW_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HFK7258251.1"
                     /translation="QQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLIS
                     HAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            292..2724
                     /gene="metL"
                     /locus_tag="ACG0BW_001264"
     CDS             292..2724
                     /gene="metL"
                     /locus_tag="ACG0BW_001264"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFK7258252.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQIQQTLRRYQCDLISGLLPAEEADSLISAF
                     ISDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLSAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(2688..3818)
                     /locus_tag="ACG0BW_001265"
     CDS             complement(2688..3818)
                     /locus_tag="ACG0BW_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFK7258253.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTDTICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITPPEIIETVAFLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHESNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGVI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGTTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAQMLSLATSRHF
                     IDWLHKQTEFTHSAPLSAGRVTQILLSLTQDYNNCASRLISD"
     gene            3951..5504
                     /locus_tag="ACG0BW_001266"
     CDS             3951..5504
                     /locus_tag="ACG0BW_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFK7258254.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPKPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMDLTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLALNGKP
                     IEDATIYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQI
                     NGMRVKDIK"
     gene            5886..6776
                     /gene="metF"
                     /locus_tag="ACG0BW_001267"
     CDS             5886..6776
                     /gene="metF"
                     /locus_tag="ACG0BW_001267"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFK7258255.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSNLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7105..9285
                     /gene="katG"
                     /locus_tag="ACG0BW_001268"
     CDS             7105..9285
                     /gene="katG"
                     /locus_tag="ACG0BW_001268"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFK7258256.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9378..10283
                     /gene="yijE"
                     /locus_tag="ACG0BW_001269"
     CDS             9378..10283
                     /gene="yijE"
                     /locus_tag="ACG0BW_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFK7258257.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSALLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSAVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKNI"
     gene            complement(10310..10927)
                     /gene="yijF"
                     /locus_tag="ACG0BW_001270"
     CDS             complement(10310..10927)
                     /gene="yijF"
                     /locus_tag="ACG0BW_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFK7258258.1"
                     /translation="MKASLALVSLLTAFTSNSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11258..12361)
                     /gene="gldA"
                     /locus_tag="ACG0BW_001271"
     CDS             complement(11258..12361)
                     /gene="gldA"
                     /locus_tag="ACG0BW_001271"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFK7258259.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12372..13034)
                     /gene="fsa"
                     /locus_tag="ACG0BW_001272"
     CDS             complement(12372..13034)
                     /gene="fsa"
                     /locus_tag="ACG0BW_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFK7258260.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13046..15547)
                     /gene="ptsP"
                     /locus_tag="ACG0BW_001273"
     CDS             complement(13046..15547)
                     /gene="ptsP"
                     /locus_tag="ACG0BW_001273"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFK7258261.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     AHHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            15856..16935
                     /gene="frwC"
                     /locus_tag="ACG0BW_001274"
     CDS             15856..16935
                     /gene="frwC"
                     /locus_tag="ACG0BW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFK7258262.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            16950..17270
                     /gene="frwB"
                     /locus_tag="ACG0BW_001275"
     CDS             16950..17270
                     /gene="frwB"
                     /locus_tag="ACG0BW_001275"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFK7258263.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17321..19618
                     /gene="pflD"
                     /locus_tag="ACG0BW_001276"
     CDS             17321..19618
                     /gene="pflD"
                     /locus_tag="ACG0BW_001276"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFK7258264.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19584..20462
                     /gene="pflC"
                     /locus_tag="ACG0BW_001277"
     CDS             19584..20462
                     /gene="pflC"
                     /locus_tag="ACG0BW_001277"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFK7258265.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPTS
                     KLLPLAKLCDEVLFDLKIMDVTQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20464..20805
                     /gene="frwD"
                     /locus_tag="ACG0BW_001278"
     CDS             20464..20805
                     /gene="frwD"
                     /locus_tag="ACG0BW_001278"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFK7258266.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20792..21643)
                     /gene="yijO"
                     /locus_tag="ACG0BW_001279"
     CDS             complement(20792..21643)
                     /gene="yijO"
                     /locus_tag="ACG0BW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFK7258267.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(21869..23602)
                     /gene="eptC"
                     /locus_tag="ACG0BW_001280"
     CDS             complement(21869..23602)
                     /gene="eptC"
                     /locus_tag="ACG0BW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFK7258268.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPIFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23785..26436)
                     /gene="ppc"
                     /locus_tag="ACG0BW_001281"
     CDS             complement(23785..26436)
                     /gene="ppc"
                     /locus_tag="ACG0BW_001281"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFK7258269.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26848..27999)
                     /gene="argE"
                     /locus_tag="ACG0BW_001282"
     CDS             complement(26848..27999)
                     /gene="argE"
                     /locus_tag="ACG0BW_001282"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFK7258270.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28153..29157
                     /gene="argC"
                     /locus_tag="ACG0BW_001283"
     CDS             28153..29157
                     /gene="argC"
                     /locus_tag="ACG0BW_001283"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFK7258271.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29165..29941
                     /gene="argB"
                     /locus_tag="ACG0BW_001284"
     CDS             29165..29941
                     /gene="argB"
                     /locus_tag="ACG0BW_001284"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFK7258272.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30002..31375
                     /gene="argH"
                     /locus_tag="ACG0BW_001285"
     CDS             30002..31375
                     /gene="argH"
                     /locus_tag="ACG0BW_001285"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFK7258273.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31642..32559
                     /gene="oxyR"
                     /locus_tag="ACG0BW_001286"
     CDS             31642..32559
                     /gene="oxyR"
                     /locus_tag="ACG0BW_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFK7258274.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32542..33942)
                     /gene="sthA"
                     /locus_tag="ACG0BW_001287"
     CDS             complement(32542..33942)
                     /gene="sthA"
                     /locus_tag="ACG0BW_001287"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFK7258275.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34274..34924
                     /gene="fabR"
                     /locus_tag="ACG0BW_001288"
     CDS             34274..34924
                     /gene="fabR"
                     /locus_tag="ACG0BW_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFK7258276.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            34924..35283
                     /gene="yijD"
                     /locus_tag="ACG0BW_001289"
     CDS             34924..35283
                     /gene="yijD"
                     /locus_tag="ACG0BW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFK7258277.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35323..36423)
                     /gene="trmA"
                     /locus_tag="ACG0BW_001290"
     CDS             complement(35323..36423)
                     /gene="trmA"
                     /locus_tag="ACG0BW_001290"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFK7258278.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36537..36727
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            36792..38636
                     /gene="btuB"
                     /locus_tag="ACG0BW_001291"
     CDS             36792..38636
                     /gene="btuB"
                     /locus_tag="ACG0BW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFK7258279.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVTVGHGNIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38581..39438
                     /gene="murI"
                     /locus_tag="ACG0BW_001292"
     CDS             38581..39438
                     /gene="murI"
                     /locus_tag="ACG0BW_001292"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFK7258280.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQKRYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9092 a         9912 c        10888 g         9681 t
ORIGIN      
        1 ccagcaaaaa ggctttggcg caatgttgag ttttgaactg gatggcgatg agcagacgct
       61 gcgtcgtttc ttgggcgggc tgtcgttgtt tacgctggcg gaatcattag ggggagtgga
      121 aagtttaatc tctcacgccg caaccatgac acatgcaggc atggcaccag aagcgcgtgc
      181 tgccgccggg atctccgaga cgctgctgcg tatctccacc ggtattgaag atggcgaaga
      241 tttaattgcc gacctggaaa atggcttccg ggctgcaaac aaggggtaaa aatgagtgtg
      301 attgcgcagg caggggcgaa aggtcgtcaa ctgcataaat ttggtggcag tagtctggct
      361 gatgtgaagt gttatttgcg tgtcgcgggc attatggcgg agtactctca gcctgacgat
      421 atgatggtgg tttccgccgc cggtagcacc actaaccagc tgattaactg gttgaaacta
      481 agccagaccg atcgtctctc tgcgcatcag attcaacaaa cgctgcgtcg ctatcagtgc
      541 gatctgatta gcggcctgct acccgccgaa gaagccgata gcctcattag tgcctttatc
      601 agcgatcttg agcgcctggc ggcgctgctc gacagcggta ttaacgacgc ggtgtatgcg
      661 gaagtggtgg gccacggcga agtgtggtcg gcgcgcctga tgtctgcggt gcttaatcaa
      721 caagggctgt cagcggcctg gcttgatgcc cgcgagtttt tacgcgctga acgcgctgcg
      781 caaccgcagg ttgatgaagg gctttcttac ccgttgctgc aacagctact ggtacaacat
      841 ccaggcaaac gtctggtggt gaccgggttt atcagccgta acaacgccgg tgaaacggtg
      901 ctgctggggc gtaacggttc cgactattcc gcgacacaaa tcggtgcgct ggcgggtgtt
      961 tctcgtgtaa ccatctggag cgacgtcgcc ggggtataca gtgccgaccc gcgtaaagtg
     1021 aaagatgcct gtctgctgcc attgctgcgt ctggatgagg ccagcgaact ggcgcgtctg
     1081 gcggctcccg ttcttcacgc ccgtacttta cagccagttt ctggcagcga aatcgacctg
     1141 caactgcgct gtagctacac gccggatcaa ggttccacgc gcattgaacg cgtgctggcc
     1201 tccggtactg gtgcgcgtat tgtcaccagc catgatgatg tctgcttgat tgagtttcag
     1261 gtgcccgcca gccaggattt caaactggcg cataaagaga tcgaccagat cctgaaacgt
     1321 gctcaggtgc gcccactggc ggttggcgtg cataacgatc gccagttgct gcaattttgc
     1381 tacacctcag aagtggccga cagtgcgctg aaaatcctcg acgaagcggg attacctggc
     1441 gaactgcgtc tgcgtcaggg gctggcgctg gtggcgatgg ttggtgcagg cgtcacccgt
     1501 aacccgctac attgccaccg cttctggcag caactgaaag gccagccggt cgaatttacc
     1561 tggcagtcgg atgacggcat cagcctggtg gcagtactgc gcaccgggcc gaccgaaagc
     1621 ctgattcagg gtctgcatca gtccgtcttt cgcgcggaaa aacgcatcgg cctggtattg
     1681 ttcggcaagg gcaatatcgg ttcccgctgg ctggaactgt tcgcccgtga gcagagcacg
     1741 ctttcggcgc gtaccggctt tgaatttgtg ctggcaggcg tggtggacag ccgccgcagc
     1801 ctgttgagct atgacgggct ggacgccagc cgcgcgttag ccttcttcaa cgatgaagcg
     1861 gtcgagcagg atgaagagtc gctgttcctg tggatgcgcg cccatccgta tgatgattta
     1921 gtggtgttgg acgttaccgc cagccagcag cttgctgatc agtatctcga ttttgccagc
     1981 cacggtttcc acgtcatcag tgccaacaaa ctggcaggag ccagcgacag caataaatat
     2041 cgccagatcc acgacgcctt cgaaaaaacc gggcgtcact ggctgtacaa tgccactgtc
     2101 ggtgcgggct tgccgatcaa ccacaccgtg cgcgatctga tcgacagcgg cgatactatt
     2161 ttgtcgatca gcgggatctt ctctggcacg ctctcctggc tgttcctgca attcgacggt
     2221 agcgtgccgt ttaccgagct ggtggatcag gcgtggcagc agggcttaac cgagcctgat
     2281 ccgcgtgatg acctttccgg caaagacgtg atgcgcaagc tggtgattct ggcgcgtgaa
     2341 gcgggttacg acatcgaacc agatcaggtg cgcgtggaat cgctggtgcc tgcccattgc
     2401 gaaggcggta gcatcgacca tttctttgaa aacggcgatg aacttaacga gcagatggtg
     2461 caacggctgg aagcggcccg cgaaatgggg ctggtgctgc gctacgtggc gcgtttcgat
     2521 gccaatggca aagcgcgtgt gggcgtggaa gcggtgcgtg aagaccatcc gctggcatca
     2581 ctgctgccgt gcgataacgt ctttgccatc gaaagccgct ggtatcgcga taaccctctg
     2641 gtgatccgtg ggcctggcgc ggggcgcgac gtcaccgcag gggcgattca gtcggatatt
     2701 aaccggctgg cgcagttgtt gtaatcctga gtcagagaaa gcagtatttg cgtaacgcgc
     2761 cctgcggaaa gcggggcgct gtgcgtaaat tctgtctgtt tatgtagcca gtcgataaaa
     2821 tgtctgctgg ttgctaaaga gagcatttgg gctttgctac tctttccgcc cgttaatgca
     2881 cataccattt tacgattttt tccctgaacc cagagttgcc actgattttt ccaccagccg
     2941 tgataagttg ttccctgaaa acgccagggc atattatcgg tatcgtcagc tatttcctct
     3001 tcaataaacc aaatctcacc ttgctgttgg cgaaacagat cagcccatgc cgtaataatg
     3061 gcacctctta aaaaacactc gtgtgaaatt acatgtgttt ttactgtagg gaagagaata
     3121 ttcagtactc tgaaatgccc gtgcgctgtg ataaccggac gcagaataac acctttagca
     3181 ttgcgtttga gcgctgcgct gccatactga atttcatcaa actgactgcg gcgtagaaat
     3241 tgtgcttcgc gcatatcaac gcgttgtaca atattactct cgtgtaactc tggttctgca
     3301 ttccctctgt atcgataatg cagactttct ccagcctgat gctgcgtgta attagtagaa
     3361 agatgaagaa tattaccgcg aagagcatca aaactgatta aaacatacag tttctgcccg
     3421 ctgctgactc cctgaaatgg cacgagaaaa gcaaccgttt cgattatttc aggaggcgtt
     3481 atttctttct gatattgttt gggtgtccat acttttttgc agttacggca ttgaatgcgt
     3541 tgcgaaccct gtggattgtg accataacaa atcgtatctg taccataaca taccgggcaa
     3601 aaatggcctt tctctgttgc aaaagtagat aagtgaactg tcaaccagtc acgaaattgt
     3661 tgctcgtcaa acaacggcgg gtaacttccg catgcccgac agtgcaatgc cggataaccc
     3721 agtcgatagt cgggccagct ataatctgtc gatgttggct gccctaaatt acggcaacca
     3781 aacgacttgc aggcattaat cgtaaacaga gtcgacatat atcaccaacg atttcacaag
     3841 gtcgttgatt ttgtccggca acgctcccct gtggatgtga ttactctcac ataaataact
     3901 ctttcagaac gtaatgtagc aatcctttaa atcactgaaa gggactattt atgaaaatta
     3961 aaatacttgc tgcgggtatc gcattaacat tgccgttctg ggcttgcgcc aaagatgtca
     4021 ccatcattta taccaacgac ctccatgcgc atgtcgagcc ttataaagtg ccgtggatcg
     4081 ctgacggtaa acgggatatt ggtggctggg caaatatcac tacgctggtt aagcaagaaa
     4141 aagcaaaaaa taaagcgaca tggttttttg atgcgggtga ttattttacc gggccatata
     4201 tcagcagcct gaccaaaggt aaggctatta tcgatattat gaatactatg ccgtttgatg
     4261 ccgtcacgat aggcaaccat gaatttgacc acggctggga caacacatta ttacagttga
     4321 gtcaggcaaa attccctatt gtgcagggca atgtttttta tcagaacagc agtaaatcat
     4381 tctgggataa gccctatacc atcatcgaaa aagacggtgt gaaaataggc gttataggtt
     4441 tgcacggtgt atttgccttt aatgacacgg tatctgcggc aacgcgagtg ggtattgagg
     4501 cgcgtgatga aattaaatgg ctacaacgtt atatcgatga acttaaaggc aaggttgatt
     4561 tgaccgtcgc cctgatccat gaaggtgttc cggcccgcca gtccagtatg ggaggcacag
     4621 atgtgcgtcg cgcgctggat aaagatattc agacggcaag tcaggtgaaa gggctggata
     4681 ttttgatcac cggacatgcg catgtgggta cgccgaaacc gattaaagtc ggcaatacgc
     4741 tcatcctctc cactgacagc ggcgggattg atgtgggcaa actggtcctc gactacaaag
     4801 agaagccgca tgattttacg gtgaaaaact tcgagcttaa aaccatttac gccgacgagt
     4861 ggaagcccga tccgcaaacg aaacaggtga tcgacggctg gaataaaaag cttgatgaag
     4921 tggtgcaaca aacggtcgcg caatcgccgg ttgaattaaa acgtgcctat ggtgaatcgg
     4981 cttccctcgg gaacctggcg gcggacgctt tactggtagc ggcgggtaaa aacacccaat
     5041 tggcgttaac caactctggt ggtattcgca atgagatccc cgcaggggcg attacgatgg
     5101 gcggcgtcat cagtaccttc ccgttcccca atgaactggt gacgatggat ctcacaggta
     5161 aacaattacg cagtttgatg gaacacggcg caagtttaag taatggcgtt ttacaggtat
     5221 cgaaaggcct ggaaatgaag tacgacagca gtaagccggt tggtcagcgg gtaatcacgc
     5281 tggctctgaa tggcaaaccc attgaagatg cgacgattta ccacattgct actcagagtt
     5341 tccttgctga tggtggagat ggttttaccg cctttaccga agggaaagcg cgtaacacaa
     5401 cgggcggtta ttacgtttat cacgccgtgg ttgattactt caaagcgggt aacaccatca
     5461 cggatgaaca gatcaacggt atgcgcgtga aagatatcaa gtaaaagtac gtattgagtt
     5521 gattcatctg tttgttacca gttgattttg cagatccagt attcggccta cgcgagggcg
     5581 caggccgaat cagatgcctt agcatcacca tccggtggca ggacgttgtt tttcgaataa
     5641 gcccaggtgt cgctgtgttg tcagaatatc agtcaaaacg tgcgaggtgg attgcgctat
     5701 agagaaatct tttatcgcct ctaattaaga tgagtttttt tcaacttccc tgatttttcc
     5761 tcaccatcat tggtcatttt tcggttgacg cccctcggct tttccttcat ctttacatct
     5821 ggacgtctaa atggatagat gttcacaaca caacatataa ctacaagcga ttgatgaggt
     5881 aaggtatgag cttttttcac gccagccagc gggatgccct gaatcagagt ctggcagaag
     5941 tccaggggca gattaacgtt tcgttcgagt ttttcccgcc gcgtaccagt gaaatggagc
     6001 agaccctgtg gaactccatc gatcgcctta gcaacctgaa accgaagttt gtgtcggtga
     6061 cctatggcgc gaactccggc gagcgcgacc gtacgcacag cattattaaa ggcattaaag
     6121 atcgcactgg tctggaagcg gcaccgcatc ttacttgcat tgatgcgacg cccgacgagc
     6181 tgcgcaccat tgcacgtgac tactggaata acggtattcg tcatatcgtg gcgctgcgtg
     6241 gtgacctgcc gccgggaagt ggtaagccag aaatgtatgc ttctgacctg gtgacgctgt
     6301 taaaagaagt ggcagatttc gatatctccg tggcggcgta tccggaagtt cacccggaag
     6361 caaaaagcgc tcaggcggat ttgcttaatc tgaaacgcaa agtcgatgcc ggagccaacc
     6421 gcgcgattac tcagttcttc tttgatgtcg aaagctacct gcgttttcgt gaccgctgtg
     6481 tctcggcggg cattgatgtg gaaatcattc cgggaatttt gccggtatct aactttaaac
     6541 aggcgaagaa atttgccgac atgaccaatg tgcgtattcc ggcgtggatg gcacaaatgt
     6601 tcgacgggct tgatgatgat gccgaaaccc gcaagctggt gggcgcgaat atcgccatgg
     6661 atatggtgaa gattttaagc cgtgaaggag tgaaagattt ccacttctat acgcttaacc
     6721 gtgctgaaat gagttacgcg atttgccata cgctgggtgt tcgacctggt ttataaatag
     6781 tgtggctttt gtgaaaatca cacagtgatc acaaatttta aacagagcac aaaatgctgc
     6841 ctcgaaatga gggcgggaaa ataagggtgt cagccttgtt ttctccctca ttacttgaag
     6901 gatataaagc taaaaccctt ttttataaag catttgtccg aattcggaca taatcaaaaa
     6961 agcttaatta cgatcaattt gatctacatc tctttaacca acaatatgta agatctcaac
     7021 tatcgcatcc gtggattaat tcaattataa cttctctcta acgctgtgta tcgtaacggt
     7081 aacactgtag aggggagcac attgatgagc acgtcagacg atatccataa cacgacagcc
     7141 actggcaaat gcccgttcca tcagggcggt cacgaccaga gcgcaggtgg gggcacaacc
     7201 acgcgcgact ggtggccaaa tcaactccgt gttgacctgt taaaccaaca ttctaatcgt
     7261 tctaacccgc tgggtgagga ctttgactac cgcaaagaat tcagcaaatt agattactac
     7321 ggtctgaaaa aagatctgaa agccctgctg acagaatctc agccgtggtg gcctgccgac
     7381 tggggcagct acgccggtct gtttattcgt atggcctggc acggcgcggg tacttaccgt
     7441 tcaatcgacg ggcgcggcgg cgcgggtcgt ggtcagcaac gttttgcacc gctgaactcc
     7501 tggccggata acgtaagcct cgataaagcg cgtcgcctgt tgtggccaat caaacagaaa
     7561 tatggtcaga aaatctcctg ggccgacctg tttatcctcg cgggtaacgt ggcgctggaa
     7621 aactccggct tccgtacctt cggttttggt gccggtcgtg aagacgtctg ggaaccggat
     7681 ctggatgtta actggggtga tgaaaaagcc tggctgaccc accgtcatcc ggaagcactg
     7741 gcgaaagcac cgctgggcgc aaccgagatg ggcctgattt acgttaaccc ggaaggcccg
     7801 gatcacagcg gcgaaccgct ttctgcggca gcagctatcc gcgcgacctt cggcaacatg
     7861 ggcatgaacg acgaagaaac cgtggcgctg attgcgggtg gtcatacgct gggtaaaacc
     7921 cacggtgccg gtccgacatc aaacgtaggt cctgatccag aagctgcacc gattgaagaa
     7981 caaggtttag gctgggcgag cacttacggc agcggcgttg gcgcagatgc cattacctct
     8041 ggtctggaag tagtctggac ccagacgccg acccagtgga gcaactattt cttcgagaac
     8101 ctgttcaagt atgagtgggt acagacccgc agtccggctg gcgcaatcca gttcgaagcg
     8161 gtagacgcac cggaaattat cccggatccg ttcgatccgt cgaagaaacg taaaccgaca
     8221 atgctggtga ccgacctgac gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt
     8281 ttcctcaacg atccgcaggc gttcaacgaa gcctttgccc gtgcctggtt caaactgacg
     8341 cacagggata tggggccgaa atctcgctac atcggaccgg aagtaccgaa agaagatctg
     8401 atctggcaag atccgctgcc gcagccgatc tacaacccga ccgagcagga cattatcgat
     8461 ctgaaattcg cgattgcgga ttctggtctg tctgtcagcg aactggtatc ggtggcctgg
     8521 gcgtctgctt ctaccttccg tggtggcgac aaacgtggtg gtgccaatgg tgcacgtctg
     8581 gcattaatgc cgcagcgcga ctgggatgtg aacgccgcag ccgttcgtgc tctgccagtt
     8641 ctggagaaaa ttcagaaaga gtctggcaaa gcttcgctgg cggatatcat cgtgctggca
     8701 ggtgtggttg gcgttgagaa agccgcaagc gccgcaggtt tgagcattca tgtaccgttt
     8761 gcgccgggtc gcgttgatgc gcgtcaggat cagactgaca ttgaaatgtt cgaactgctg
     8821 gagccaattg ctgacggttt ccgtaactac cgcgctcgtc tggatgtttc taccaccgag
     8881 tcactgctga ttgataaagc acagcaactg acgctgaccg cgccggaaat gaccgcgctg
     8941 gtgggcggga tgcgtgtact gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact
     9001 gaccgcgttg gtgtattgag taatgacttc ttcgtgaact tgctggatat gcgttacgag
     9061 tggaaagcga ccgacgaatc gaaagagctg ttcgaaggcc gtgaccgcga aaccggcgaa
     9121 gtgaaataca ctgccagccg tgcggatctg gtctttggtt ctaactctgt cctgcgtgcg
     9181 gtggcggaag tttacgccag cagcgatgcc cacgagaagt ttgttaaaga cttcgtggcg
     9241 gcatgggtga aagtgatgaa cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag
     9301 cgactgcctg atggcagtcg ctgaagtttc tttaccggcg tatagtgtcc acaggaaaac
     9361 tacacactgg atctctcatg tctgccgcag gaaagagcaa cccactggca atcagtggcc
     9421 tggttgtgct cacacttatc tggagttata gctggatttt catgaagcaa gtcaccagct
     9481 acatcggtgc cttcgacttc accgccttac gctgcatttt cggcgctctc gttttattca
     9541 ttgtcctttt attacgtggt cgcggaatgc gcccgacacc gtttaaatac accttagcca
     9601 ttgccctgtt acaaacctgt gggatggttg gcctggcgca gtgggcgctg gttagcggag
     9661 gagcggggaa ggtggcgatc ctgagctata ccatgccgtt ctgggtggtg attttcgccg
     9721 cgttgtttct cggtgaacgc ctgcgacgtg ggcaatattt cgcgatactg attgccgctt
     9781 tcggcttatt tttggtgttg cagccgtggc aactcgattt ctcttcgatg aaaagtgccc
     9841 tgctggcaat cctctccggt gttagttggg gggcgagcgc gattgtcgcg aaacgcctat
     9901 acgcccgcca tccacgcgtg gatttattgt cgttaacatc ctggcagatg ctttatgcgg
     9961 cgctggtgat gagtgcggtc gctttactgg tgccacaacg tgaaattgac tggcagccca
    10021 ccgtgttctg ggctctggcc tacagtgcga ttctggcgac ggcgctggcg tggagcttat
    10081 ggttgtttgt attgaaaaac ttgcctgcca gtattgccag cttaagcaca ctggccgttc
    10141 ccgtttgcgg cgtactcttt tcctggtggt tgctcggtga gaatccgggg gccgttgaag
    10201 gtagcggtat tgtgctgatt gtgctggcac tggcgctggt gagccgtaag aaaaaagaag
    10261 ctgtcagtgt aaaaaacatc tgaatttttt cttcatgtgg ggcgatcgct tatttaacaa
    10321 aataccgata gtgccccacc atccgccagc taaacagcac gtcttcttcc tgcgcgcctg
    10381 cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc aaagccatct gacaccaccc
    10441 caatatgcgc cagcccgtta tccagtcgcc aggagacaat atcgcccgct tgatagtcac
    10501 tggggttctt gctggtgggg cgtgttttat catggcggct aaaccaggtt tccagattag
    10561 gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa ctgccatttt tgcgggtact
    10621 cagcaaaatt cttcgccata tcttcgtgaa ccagtttctg caaatcgacc ttctggctgc
    10681 gcaaggcgcg gatcaccaca tcggaacata cgccgcgttc ttgcgggaca tcaccgccag
    10741 gataggtaag ctgcacatac gccggatcgt aaaatagcgt gctgccgatt tgctgtctgg
    10801 caccgtctgc gatggcaagg ttggtgttgg cctggatttg taccacggtt ggtggaacgg
    10861 ccggagattt taaggagttg ctggtaaacg ccgttagcag gctgaccagc gccagtgaag
    10921 ctttcatctg gttcgtccgt gaaaataaga gtcacagatt cgaccttccg ggctaacgat
    10981 tgttctgtag agaaaatgtc cagaagcgga taaaaaattc ctgaacaacg tgtgagtttc
    11041 agacgggtga taacgtataa aaagtgctcg aacagtcccc tctccccttt ggggagaggg
    11101 ttagggtgag gggaacaggc cagctccggg gcgaatattt accctcactc cgaccctctc
    11161 cctgaaaggg cgagggggaa aaacatgccc gcagtgacga gtgagccact ttgcctgcac
    11221 tctcaaactc ccggacaagc cgggagtttg gagtaggtta ttcccactct tgcaggaaac
    11281 gctgaccgta ctggtcggct accagcagtg cagcgtaaac ctgatctggc gtcgcgccgc
    11341 caggcatgtt gtggatggtt tcaccttctg cacatgccgc ttctgccaca attcgcattt
    11401 tcgccgggac atcttcttta atatccagtt gagcgagagt tattggcaaa cctaccgcat
    11461 ggctaagtgc agctacggtt tcgatttcct ccaccggcgc gttttccaga accagctgcg
    11521 tcagcgtacc gaatgccact ttttcaccgt gataatagtg atgcgcatct ggaatagcgg
    11581 tcaggccgtt atgtactgcg tgcgccgcag ccagacctcc gctttcaaaa cccacgccgc
    11641 tcaaataggt gttcgcttca atcacgcgct ccagcgccgg agtcactaca tgctgttcgg
    11701 cggcaagcat cgctttttcg ccttcttcca gcagggtgtt gtagcacagt tcagccagtg
    11761 ccagcgccgc ctgggtgcac ttgccgcccg ccatggtggt cgcgccgcta cgagagcaag
    11821 cacgcgcttc aaaccaggtt gccagcgcat cgccgatacc cgccgctaac agacgtgcag
    11881 gtgcgccagc gacgattttg gtgtcgacaa tgaccatatt cgggttattt ggcaacatca
    11941 gatagcggtc aaattcacca tcatcggtgt agataacaga caatgcgctg cacggtgcat
    12001 cggtagaggc gatagtcggt gcgatcgcta ccggaacacc catgaaatgt gccagtgctt
    12061 tggcagtatc gagggttttt ccgccaccga taccgagaat tgcgccacac tgcgcagtct
    12121 ccgcgatgcc acgcagacgg tcgatctcat tttgcgaaca ttcaccgcca aacggcgcaa
    12181 tttctactac cagtccagca tctttaaaac ttttctcgac agtggattga gcaaaaccta
    12241 aaacaaattt gtcacccacc actaaccagc gttctgccag cggcttcagg tattcgccca
    12301 gacgattaat cacatcagcg ccctggatgt atttacccgg tgattgaata atgcggtcca
    12361 taattgctcc tttagagatg agtagtgcca aatgcggcat tccagtcgtg ttcgaacttc
    12421 tctatagctg actctaccgc aggggtgttg agcatttgtt gcgctacatc taagggcagg
    12481 gtgatggatt cacatcctgc cagtaaacag tccagcgcct gacgcggcgt tttaaagctg
    12541 gccgccagca ccatgctttc tggtgcgtgc atttctaaca gcgcttgcag ctcctgaacc
    12601 gtacgaatgc cgtctccgcc ctgggcatct acgcggttaa catacggagc aacgtatttt
    12661 gcccctgcca gtgcggctaa taacccttgt gcggcgctat atacagcggt gccaagtgta
    12721 gtaataccct cttttttcag tattttaatt gctgccagac cttcggaagt caccgggatt
    12781 ttaaccacaa tgcccggaat ggcgtcgcgc aggcgcttcg cttcttccac catcccctgc
    12841 gcgtcgcggc tcattgtctg agcaaacaga atgccctcat caccaatcgc tttttgcaga
    12901 cgcggcagca cttcccatat ggactccttg ctggcagcga taatgctcgg gttagttgtc
    12961 accccggcaa tcgggaatat gcgtgccaga cgttcgactt ctgcgacgtt agcggtgtcc
    13021 agatacagtt ccatgatgtt tccttttaca gttccagttc atgttgcagc aggctggcga
    13081 tagcgtcggc agaggcggcg ttaaccagcg cgttacggaa ttcttcgtgc atgatgcggc
    13141 gagcgaggcg cgagaaaatg cgcatgtgct gatcgcccgc agcgtgtttg ttcagcgtta
    13201 acataatgat gaattgcgct tcatcatcgc cccagcgcac cggcgcttgc agacgcgcta
    13261 cgctgatggt ggattgctca atatgttccg atttgctgtg gggaatggca aaactaaagc
    13321 ccagaccggt agagaaaacg gcctcgcgcg cccacaagtc ggcttccagt ttacgcggat
    13381 agcggcagcg gcccgccagc agcaggttat cggtcatgcc tttgagcact tcttctttgc
    13441 tgcgccagtc gctttccagt gtgatgcact cggcggtgac cagcggtgcg tcttgttggg
    13501 tcatgcggaa ttgcgccagc aggtgttcca cttccagcga agtacggcag gccattgcct
    13561 ggttgagcaa cttgcggcac tcacggctat caagttgcgc cattcgcgct ttcgccgccg
    13621 gaattgatgg tgcgctcatg ctcagctcat ccagccctaa accgaccagc aatggcagca
    13681 cggaaccttt cgctcccagt tcaccgcaca gaccaatcca tttgccctgg cgatgtaccg
    13741 cctgcacggc gtaatcgagc gcccgcaaga atgctggatt caggctgtta tagtgacgag
    13801 taaccttagc gttatcgcga tccactgcca gcagatattg cgtcaggtca ttactaccaa
    13861 tactaaagaa atcaatctct tcacagcatt gatcgatgat gaacatcacc gacggcactt
    13921 ccagcatgat gccgagctgg atcttctcat caaacggaat gtgttcgtta cgcagttgct
    13981 gtttggcttc cgccagtttt tctttcaccc ataagatctc ttccattgaa gagatcatcg
    14041 ggatcatgat tttcaggctg ccgtgagcgg aggcacggag aatcgaccgt agttgtgtgg
    14101 taaataacga cgcgtactct tcataaatac gcacggcgcg ataaccgagg aacgggtttg
    14161 cctctgcggg aatgttcaga taatcaacgg gtttgtcacc gccaatatcc atagtgcgca
    14221 caataatgct gcgtccgttg gcggactcca gcgcctggca aaaaatgttg tacagctcgc
    14281 tttcgcccgg tgcactggtg cgatccatat agagcatttc agtgcggaat aaaccaacgc
    14341 cttccgcacc attgccgaat gcggcctgcg cttccacgga gtgagcgatg ttagcggcaa
    14401 tttcaatgcg gataccgtca gcggtacggg cttgttgggt cagccagaca cgctgttgct
    14461 cacgcagggc gtcctgtacg cgggcttcct gctgataata acgagctacg gcttcccctg
    14521 gctcaaccac aatcgcccca gcgttgccgt cgatataaat cgtttgatgc tgccacggag
    14581 tgagggcatc aatatccaca ccaaccagcg tcggaatgtt gaacgaacgg gcaaggatca
    14641 ccgtgtgtga cgtggtgccg ccgcttttga gcaacaatcc tttgaggtga tttttatcca
    14701 gttcgaggaa ctggctgggg gtcagttcat cagccataca aatggcgggc tgcgtcagtt
    14761 tgcccggtgc cgggaagcgt tgctcaccgt agatttgctg gagtaactgg aagcagacgt
    14821 cgcgtacgtc cagggcacgt tcttgcaggt agctgctgct ggaacgggcg aactcttcgc
    14881 agaagtggtg tgcactggcg acaatcgctt cggcacagct taatccctcg ctgacgcctg
    14941 ccagtaagtg ctgacgtaaa gaagtatcgc cagccaggga tcggtgagct tccagtatcg
    15001 cgctggtagc accgtcgcta tccagcagac gaaattcaat gtttttcatt accagtgtca
    15061 ggccgttttc cagcgcggat tgctcggcgt caacgccttt ggcagcagga agattaccca
    15121 gcgcattgag atctaaagaa gagatcggcg tcagaatgcc gcctgcacta ccgctgcaca
    15181 cggtgcgggc gcggataatt tgcggattca gattggtcag tgaaaccggc agtggttcca
    15241 gttcgtcaga tttaacttcc gccagcggcg catcgcagtg ggggaattca tcgcgcagcc
    15301 attggcttaa acgctggtgc gcttcctgtt cgtcggcccc ggaaatcagt aactggcagt
    15361 tatcgcccgc cagcgtgtcg gtgccaatca gcgcaagggc gcttttggcg ttgcccttgc
    15421 ggtcagtgcg caggttatgc cactcaattt gtgatgaaaa agtattacac agcgtttcaa
    15481 cgtggcttgc cggacgcgca tgtacgccgt taggtagctc acaaataaat tccacaatca
    15541 gggccattgc ctttctcccg gtacggtttt ctgtttacag gataagagcg cacggcaacg
    15601 gcctgccatg tgacaaatct gccaaaagct ggacaaatgt aatgtaaccg tcaatttgcg
    15661 acgcgtctca caagacaccg ttctgcggca tgcttccggg ttatcgcaag ttatgaggcg
    15721 gatcgcattt ttgtactgat attacaaaag tccagtaaat ggccttttta tccactgttt
    15781 gctccgcctg cgattgccta ttgtttcctc catcaaaaat atggacatgg gcttttgcaa
    15841 gcccgggagc agcttatgaa tgagttggtg cagatcctga aaaatacccg tcagcactta
    15901 atgacgggcg tttcacacat gattcccttc gtggtatcgg gcggtatttt gctggcggtt
    15961 tccgtcatgt tgtatggcaa aggcgcagtg ccggatgccg tagccgatcc gaatctgaaa
    16021 aaactgtttg atatcggcgt tgcgggtttg acgctgatgg tgcctttcct cgccgcttac
    16081 attggttact ccattgcaga gcgttctgcg ctggctccgt gcgctatcgg tgcctgggtt
    16141 ggtaacagct ttggtgcggg cttctttggt gcactgatcg ccgggattat cggcggcatc
    16201 gtggtgcatt acctgaagaa aattccggtg cataaagttc tgcgctcggt gatgccaatc
    16261 ttcatcattc ctatcgtcgg cacactgatt accgcaggca tcatgatgtg ggggctgggc
    16321 gagcctgtgg gggcattgac caacagcctg actcagtggc ttcaggggat gcagcagggc
    16381 agcattgtta tgctggcggt gatcatgggc ctgatgctgg cgttcgatat gggcggtccg
    16441 gttaacaaag tggcctatgc cttcatgctg atttgcgttg ctcagggtgt ttataccgtg
    16501 gtggccattg ctgccgtggg tatttgtatt ccgccgctgg ggatggggct ggcgacgctg
    16561 attggtcgta aaaatttctc cgcagaagag cgcgaaaccg gtaaagcggc gctggtgatg
    16621 gggtgcgttg gggttactga aggggcgatt cctttcgccg ctgccgatcc gctgcgtgtt
    16681 attccttcca tcatggtcgg ttcagtttgt ggtgcggtaa ctgcggcact ggtcggtgca
    16741 cagtgctatg caggctgggg tggtctgatt gtgctgccgg tggttgaagg caagctgggt
    16801 tatatcgcag cagtggctgt cggagcagtg gtgacggcag tctgcgtgaa cgtgctgaaa
    16861 agtctggcgc gtaaaaatgg gtcttcgact gatgaaaaag aagacgacct ggatttggat
    16921 tttgaaatta attaattgag gaacgagcca tgacgaaaat tattgcagta accgcgtgtc
    16981 catctggtgt tgcccatacc tatatggcgg cagaggcgct ggaaagtgcc gcgaaagcga
    17041 aaggctggga agtgaaagta gaaacccagg ggtcaattgg tctggaaaat gaactgacag
    17101 cggaagacgt ggcgagcgcc gatatggtga ttctgaccaa agatatcggc atcaagtttg
    17161 aagagcgttt tgctggcaag accatcgtgc gcgtcaacat cagcgacgcg gtaaaacgcg
    17221 ctgatgccat tatgagcaaa attgaagctc acctggcaca aaccgcttaa gttcttatcc
    17281 cgcccgtaag ggcgggttcg ctttcccaca ggagttcctc atgacgaatc gtatctctcg
    17341 cctcaaaact gcactgtttg ccaatacccg tgaaatctcg ctggagcgtg cgctgcttta
    17401 taccgccagc catcggcaaa ccgaaggcga accggtgata ttgcgccggg cgaaagcaac
    17461 agcgtatatc cttgaacatg ttgaaatttc gattcgtgat gaagaactga ttgccggtaa
    17521 ccgcaccgta aaaccgcgcg ccgggattat gtcgccggaa atggaccctt actggctgct
    17581 gaaagagctg gatcaattcc cgacgcgtcc gcaggaccgc tttgctatca gcgaagaaga
    17641 taaacgtatc taccgtgaag agttgttccc gtactgggaa aaacgttcga tgaaagattt
    17701 cattaacggg cagatgacgg atgaagtaaa agccgcgacc agcacgcaga ttttcagcat
    17761 caaccagacg gataaaggcc aggggcacat tattattgat tacccgcgcc tgctgaatca
    17821 cgggctgggt gagctggtag cgcaaatgca gcaacattgt cagcaacagc cggagaatca
    17881 cttttatcag gccgcgttgt tactgctgga agcctcgcag aaacacattt tgcgttacgc
    17941 cgaactggcg gaaacgatgg cggcaaactg cacagatgcc cagcgtcgcg aagagctgct
    18001 gactattgcg gagatctccc gccataacgc gcaacataag ccgcagacgt tctggcaggc
    18061 gtgccagtta ttctggtaca tgaacatcat tctgcaatac gaatccaacg ccagttcgct
    18121 gtctttaggg cgcttcgacc agtatatgtt gccgttctat caggcatcat taacccaggg
    18181 cgaagatccg gcgttcctga aagaactgct cgaatcttta tgggtgaaat gcaacgacat
    18241 cgtgctgttg cgctccacca gtagcgcgcg ttatttcgcc ggtttcccga ccggctatac
    18301 cgcactgctc ggcgggttaa ccgagaacgg acgtagcgcg gtgaacgtac tttcgttcct
    18361 ctgtctcgac gcctatcaaa gtgtgcaatt accgcaaccg aacctggggg tgcgcactaa
    18421 cgccttgatc gacacgccgt tcctgatgaa aaccgccgaa accattcgcc tcggcaccgg
    18481 tattccgcaa atctttaacg atgaagtggt ggtgccagcg ttcctcaacc gtggcgtttc
    18541 gctggaagat gcacgcgact attccgtagt gggctgtgtg gaattatcta ttcccggcag
    18601 aacctacggc ttgcatgaca tcgcgatgtt taatctgctg aaagtgatgg aaatctgcct
    18661 gcatgaaaat gaaggcaacg ctgcgctgac ttatgaaggt ttactggagc agatccgcgc
    18721 caagatcagc cactacatca ccctgatggt tgagggcagc aatatttgcg atatcggtca
    18781 ccgtgactgg gcacctgtac cgctgctctc atcgtttatc agcgattgtc tggaaaaagg
    18841 ccgcgatatt accgatggcg gtgcgcgtta taacttctcc ggcgtacagg ggatcggtat
    18901 cgccaacctg agcgattctc tccatgcgtt gaaaggtatg gtttttgagc aacagcgttt
    18961 aagttttgac gaattgctgt cggtattaaa agccaacttc gcaacgccag aaggcgaaaa
    19021 agtccgtgct cgcttaatta accgcttcga gaaatacggt aacgatatcg acgaggtgga
    19081 taacatcagc gccgaactgt tacgccacta ctgcaaagaa gtggaaaaat accagaaccc
    19141 gcgcggcggc tacttcacgc cgggatcgta taccgtttct gctcacgtcc cgttgggatc
    19201 ggtggttggc gcgacgccag acggtcgttt tgccggggaa caactggcag acggcggctt
    19261 gtcacctatg ctgggtcagg acgcacaagg gccaacggcg gtactgaagt cagtcagtaa
    19321 gctcgataat acgctgctgt ctaacggtac gttgctgaac gtgaaattca ctccggcgac
    19381 cctggaaggt gaagcgggat tacgcaaact ggccgacttc ttacgggcgt ttacccagct
    19441 taagttgcag catattcagt ttaacgtggt gaacgccgac acgttgcggg aagcgcaaca
    19501 gcgcccacaa gattatgccg ggctggtggt gcgcgttgcc ggatacagcg ccttctttgt
    19561 cgaactgtcg aaggagatcc aggatgacat catccgccgg acagcgcatc agctgtaacg
    19621 ttgtggaaac gcgccgcaat gatgtggcgc gcattttcaa cattcagcgt tattcactga
    19681 atgacggcga gggcattcgt acggtggtct tttttaaagg ctgtccgcat ctttgcccgt
    19741 ggtgtgctaa tccggagtcg atctccggca aaatccagac ggtacgcaga gaggcgaaat
    19801 gtctgcactg tgcgaaatgt ttgcgtgatg cggatgaatg cccgtccggg gcgtttgaac
    19861 ggattggtcg cgatatcagc cttgacgcgc tagaacggga agtgatgaaa gatgacattt
    19921 tctttcgcac gtccggcggc ggcgtcacgc tttctggcgg cgaagtgtta atgcaggcgg
    19981 agtttgctac ccgtttttta cagcgacttc ggctttgggg ggtgtcatgc gccattgaaa
    20041 ctgccggaga cgcgccaacc agcaagttgt taccgctggc gaaattgtgc gatgaagtgt
    20101 tgttcgattt aaaaattatg gatgtgactc aggcgcggga tgtggtgaag atgaacctgc
    20161 cacgcgtgct ggagaatttg cgtttgctgg tgagtgaggg tgtcaacgtg atcccgcgtt
    20221 taccgctgat ccctggtttc acactcagcc gggagaatat gcagcaggcg ctggatgtac
    20281 tgatcccgct gaatatcagg cagatccatc tgttaccgtt tcatcagtac ggcgaaccga
    20341 aataccgcct gctggggaaa acatggtcga tgaaagatgt tgctgcgccg tcgtcagccg
    20401 atgtgacaac gatgcgcgaa atggcagaac gggccggatt tcaggttacc gtgggaggtt
    20461 aaaatggcat acctggtggc agtaaccgcc tgcgtaagcg gcgtggcgca tacatatatg
    20521 gcggcggaac ggctggaaaa gttgtgccag ttagagaagt ggggcgtcag cattgaaacc
    20581 cagggcgcgt tgggaacgga gaatcgttta gcggacgagg atatccgccg ggcggatgtt
    20641 gcgctgttaa ttacggatat cgagcttgcc ggtgctgagc gatttgaaca ttgccgctat
    20701 gtgcaatgca gcatctacgc tttcctgcgt gagccgcagc gggtgatgag cgcggtgcgc
    20761 aaagtgcttt ctgcgccgca gcaaacccat cttattctgg agtagtcggt ttttctgtca
    20821 gttggctgtg gtactgacga cgatattccg acggcgagcg ttcggtattt ttacgaaaca
    20881 gacggcagaa gtagttgctg tcgacaaagc cgcaggcgtg cgccacttct tttaccttca
    20941 ggtcatagcc tttcaataac gtcttagcgt gctccagtcg cgtgtgattc aggtattcgt
    21001 taaaaccaat ggcccctgtt ttttgaaaca ggtgcgagag atagtttggt gaaatataga
    21061 acgcctgtgc gacagattcg cgggtaagcg cggaggcata gcgttcgtcg atataatcgc
    21121 gaatagcttc gaatagtgcc tggctgcgtg aggcggtctg gatttggctg ccaagcaaat
    21181 cgcggcagtg gctaagcagg ctggcgacga taagccttgc cgtttgctgc tcctgcggct
    21241 gcatttgcat ttcattgagc gtttgtagca gaaaagaacc tatgcgtggg ccgcgccggg
    21301 cgacgtgttg cttcgccaga ttttgatatt gtttgccatc ccattgcacg acgctgaagc
    21361 cgagttgctg tttgccaaac agcacgctaa aggtagtggc gggggcttgc cattgtggaa
    21421 aattccagcc accagcagga acgtacagca catcgccggg aactaacgtt gctccagcac
    21481 cggtatcaac aaactcacct tccagcacaa tttccagccg tggaaaatcg acctgatacg
    21541 caagatcggg caccgggccg ttactgctgg caaagtaaat ctggcgcagg gacagcgggc
    21601 cgttgatcag gcgggagagc agatagctga cgtcgtgata catgttattc ccttaggtat
    21661 gtagacggga aaacagtaac tggcattatt aaaaaaggcc acctgaaaag tgtggcctga
    21721 ggggagttcg atgtaaacat tgcggcagtt tcagtcgact tgtattgccg gatgcggcgt
    21781 aaacgcctta tcctgcctac cgccgcgatc tgtaggccgg ataagacgcg tcaagcgtcg
    21841 catccggcaa atagcacttg gctgataatt actgattacc cacctgatcg ccatacggca
    21901 gtgtgtcgta atcgatcagc gcatttttct tatacgggtt accaatccag cgggtagttt
    21961 ctttgaactg cggattcacc actgaacggg ttggatcgta accgtcgtaa gataaacccg
    22021 ccaaatctga ccaggtgtgg atcagttccg ccaggctgta tttacgatca acatcctgcg
    22081 agaaatcacg cggatgagtc gcttgccatt tttctgacgt ccacagcagg aacggaatgg
    22141 tgtacatatg acgcgtcggg ttgtcctcat tacgcccctg agttttgtgc ggcggcgtgt
    22201 cgtaaacctc ttcaccgtgg tcagagaagt aaaccaggaa accgttcggg tctgctgctt
    22261 taaagtcttt aatcaggctg gcaaccacat gatcgttata caggttagcg ttgtcataat
    22321 cgttatatga ctccagctct tctgcgctta gtcccggcgg aacatgatcg gtattgccat
    22381 caaacttgcc ctgattttcc gggtagcggt atttgtattt gatatgcgta cccagcagat
    22441 gaacgatgat cagtttcttc ggcgcagggt cattcagcac ttcctggaac ggcttcagca
    22501 cgttggtgtc gtattcacgc gcactctgcg tacgttgctg gttcatgtag tactgcttgt
    22561 cggtctggcg cgaaaatacc gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg
    22621 tgatccagaa ggttttataa cccgcctgtt tcatcatgtt catcagcgac ggctgcgtca
    22681 gatacagatc cgggttcttt tcattggcaa aggtcagcgc ctgttgcagg atttcaatgg
    22741 tgtacggacg agacgtcacc acgttattaa acacggtcag attcggatcg gttttatgca
    22801 gcgcatccag ctccggcgtg gtttcacgcg gataaccgta cagactcatg cggccacgct
    22861 gggtcgactc gccaatcacc agcactaaag tgcgcggttc gttacccgat tcatctttga
    22921 aattcgccag cggcggcaag gcattatttt cattcagtag ctttgttagc gagtttagtt
    22981 gctgacgata ctgataatag ccggtcagga attgccacgg tgcggcaggc tccatacgcg
    23041 atgccaggtt atccaacgtt ttctcaaatg gcttgttttt gataaacgta ttcatggcga
    23101 tcggatgcag aatcaagcca taaagtaggg caaaagagac aacataacgc cacggctttg
    23161 gaatatagac cgggcgcagg cgtgtccaca gcagaactgc caccgccgta taggccagcg
    23221 cgataagcac aattttcagg ctgaaatact ggcttaaata ctcgctggct tcgttggtgt
    23281 tggtttcaaa catcacaaac agaacgctct gcgagaactc ctgaccgtag atgacgtagt
    23341 agcacagcgc cgccagagag gccgcccata gcaccacgcc gatcactgcg gcaataattt
    23401 taatccgctt cggaaagagg aatatcggga tcaaccacag cgaactgaat aacagcgagt
    23461 cgcgaatgcc gttagtgcca ctataaccac tgatgtaaat aatggcctgt agcagagtag
    23521 agaaaaacca aaagtagagc agtgcccaac ccagggcttt ccagctaaaa aggggtttag
    23581 cttggacttc tgtggaatgc atagtaagaa cctgtcttga aaaaaatatc gccgaatgta
    23641 acgacaattc cttaaggata tctgaaggta tattcagaat ttgaataaaa tgcagacaga
    23701 aatatattga aaacgagggt gttagaacag aagtatttca gaaaaccctc gcgcaaaagc
    23761 acgagggttt gcaggagggg aagattagcc ggtattacgc atacctgccg caatcccggc
    23821 aatagtgacc attaacgcct gttcgacgcg aggatccggt tcctggcctt ctttttctgc
    23881 ctggcgggag cggtgcagca actcggcctg caatacgttc agcgggtcgg tgtaaatatt
    23941 ccgtagctga atagactctg caatccacgg cagatcggcc atcagatggg aatcgttggc
    24001 aatcgccagc accactttga tgtcttcttc ttgcagattg cgtaactctt tacctaacgg
    24061 ccacagtgct ttgtctacca gacgttgatc atagtactcc gccagccaca ggtctgcttt
    24121 ggcgaagacc atctccagca tgccgagacg cgtcgagaag aatggccaat cgcggcacat
    24181 agcctccagc tcgctctgtt tgccatcttc gaccactttt tgcagtgccg tacctgcacc
    24241 cagccaggcg gggagcatca ggcggttttg cgtccatgca aaaatccacg gaatggcgcg
    24301 cagcgactcg acgccgccag tcgggcgacg tttcgccgga cgtgaaccca acggcagttt
    24361 gcccagttcc tgttccggcg tagcggagcg gaagtaaggc acaaaatctt tgttttcacg
    24421 tacgtagcca cggtagagat cgcaggagat gactgacagt tcatccataa tgcgacgcca
    24481 gctctctttc ggctcaggtg gtggcagcag gttggcttcc agaatcgccc cggtgtacag
    24541 cgacaggctg ctgacggtga tttctggcag accatattta aagcggatca tctcgccctg
    24601 ttcagtcacg cgcaggccgc ctttcaggct tcctggcggt tgtgacagca gcgccgcatg
    24661 agcaggtgcg ccgccgcgac caatggaacc gccgcgaccg tggaacaacg tcagttcaat
    24721 acccgctttt tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg cccaggaagc
    24781 cgccatcacg cccgcatctt ttgctgagtc ggaatagcca atcatcacca tctgtttgcc
    24841 ctgaatcagg ccgcgatacc agtcgatatt cagtagctga gtcatgacat cgttggcgtt
    24901 gttcaggtca tcgagagttt caaacagcgg agcaaccggc atcgcaaagc cgatacccgc
    24961 ttctttcagc agcaggtgga cagccagcac gtcggacggc gttttcgcca tcgagatcac
    25021 gtaggcggca atggagcctt gcggtgcttc ggcaatcacc tggcaggtat cgagcacttc
    25081 gcgcgtttcg gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg agttcagttc
    25141 gcggatcagg aatgcctgtt tgtcggcctc tgaccagctt tcataatcgc cgatgccgag
    25201 atagcgggtc agctcgccca atgcttcggt atggcgggtg ctctcctggc ggatatcaat
    25261 gcgcaccagc ggtacgccga aacatttcac gcgacgcaga gtgtcgagca gatcgccgtt
    25321 ggcgataata cccatgccac atgcctgaag ggactggtag caagcgtaga gcggttccca
    25381 cagttcttcg ttttgtgtca gcaggccttc tggttttggc agttcttcgc ctttcaggcg
    25441 cgcttccagc catgcctgtg tcgccatcag gcgagaacgc aggtttttca tcagatagcg
    25501 atacggttct gcggcacctt cttcgccgac cagcgccagc agttcagggg ttgcttcaac
    25561 catcgacagt tcagaaacca gcacctgaat atctttcagg aacagatcgg tggctttcca
    25621 acggctgagc agcaggacgt ggcgggtgat atcggccgtg acgttcgggt tgccgtcgcg
    25681 gtcaccaccc atccacgagg taaaacgcac cggaacaaat tcgacgggca gtttgtagcc
    25741 gaggttctct tccagttgtt cgttcagttc gcgcaggtaa ttcggtacgc cttgccacag
    25801 gctgttttct actacagcaa agccccattt ggcttcatct accgggcttg ggcgcagctt
    25861 acggatttca tcggtatgcc atgactgggc gattaactgg cgcaggcgac gcatcagctg
    25921 gttgtgttcg tagtcagcga tatctttgtt atcgagctgt tttaaacagg cgttcacttc
    25981 caccattttg tggatcagtg tacgacgggt aatttcggtt gggtgagccg tgaggaccag
    26041 ttccagcgac agcgattcca ctgctttttt gatggtgtct tcgctcagtt ccggctggtt
    26101 tttcagttta cgcagggtgc gggcgatcac ttccgggttg ctggcagctt cgcctttcgg
    26161 cgaaatgctg tggtattgct cggcggtgtt ggccaggttc aggaactgac taaacgcacg
    26221 cgcaacgggc agcagctcgt cgttcgacaa attttgtaag gtggtgagca actcctggcg
    26281 gttagcatca ttgccagcgc gtgaagattt cgacaactta cggatagttt ctacgcgttc
    26341 aagaatgtgt tctcccaacg catccttgat ggtttctccc agcactttgc cgagcatact
    26401 gacattacta cgcaatgcgg aatattgttc gttcatatta ccccagacac cccatcttat
    26461 cgtttgatag ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg gctttgcttt
    26521 tcgtcgtctt ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca
    26581 gcaaacgaat aaatagcagg aatttacgcc attaaattca cgacgcttta aataagcgta
    26641 acttatggga atgttaaaaa atctcctcaa ttaacaccaa agagttatga gttgtagtta
    26701 ccatttgggc atcatttgag attgctgaca aactcgctta cgggtcagta tgggcacggt
    26761 tttcccctca ccctaaccct ctccccgaag gggcgagggg actgtccgtg cgcttgcatg
    26821 gctttgtcat cagcctgatt tgctttttta atgccagcaa aaatggtgaa ttacctgggt
    26881 tatcagttcg cgggtgggct tgataaaccg tgtttccaga tattcatcag gttgatgagc
    26941 ctgattaatt gagccagggc ccagaaccag cgtcgggcat aacgtttgaa taaacggcgc
    27001 ttcggtacag taattcacca cttcggtttt tgctccgagc aatttctcaa ccacttcaac
    27061 cagttgatga ttcggtgggc attcatagcc agggatcggc ggatgcagct cgtcgaccgt
    27121 cagacgaccc ggccagcgtt cgctcaccgg agccagcgca tcgttgagta ggccattaag
    27181 ttcattgagt gtcatgcccg gtagcggacg aatatccata tgcaattcac agcaagcgca
    27241 aatacggtta gatgcatcgc caccgtgaat atgcccgagg ttgagcgtag ggtatggcac
    27301 ggtaaacgcg tcgtagtgat agcgttcttt caggttatcg cgcaattgca aaatatgccc
    27361 gatggcgtcg tgcatcagtt cgatagcgtt aactccgcgt gctggatcgc tggagtggcc
    27421 cgactggccc tgaatacgga tggcgttaga gatatgacct ttatgtgcgc gtaccggttg
    27481 tagtgacgtc ggttcgccaa tgatggcgca atccgggcgc agggcggtag tttcggcaaa
    27541 ataacgcgct ccggccatac tggtttcttc atcggcagtc gccagaatgt agagcggttt
    27601 tttcagtttc gtgacgtcga catcgcgtag cgcatcaagg ataaacgcaa aaaagccttt
    27661 catgtcggcg gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg taaacggatc
    27721 gcgcgtccag cgtccgtcat caaatggcac cgtatcggta tgccccgcca gcaacaaacc
    27781 gccagccccc tgtccgcaac tggccagcat attaaatttg ttgcgagttc ctggcacagg
    27841 ctgcacttcc acattgaagc ctaaatcttt aaaccagtcc gccagcagag tgattaaatc
    27901 tgcattgctt tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg caatcagagc
    27961 acggtaaatc tcgataaatg gcggtaattt gtttttcatt gttgacacac ctctggtcat
    28021 gatagtatca atattcatgc agtatttatg aataaaaata cactaacgtt gagcgtaata
    28081 aaacccacca gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga cataagaagg
    28141 tgaatagccc cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc tggcgcagag
    28201 ctagtgacct atgtaaatcg ccatccgcat atgaacataa ccgctttgac tgtttcagcg
    28261 caaagcaatg atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa aggcatcgtt
    28321 gatctgccgt tgcagccgat gtcggatatc agcgagttta gcccaggggt ggacgtggtg
    28381 tttctcgcca ccgcccatga agttagccac gatttagcgc cgcaatttct tgaagccggc
    28441 tgcgtggtgt tcgacctttc cggcgcgttt cgtgttaacg acgccacctt ctatgaaaaa
    28501 tattacggct ttacccatca gtatccggaa ctgctggaac aggcagccta cggtctggcc
    28561 gagtggtgcg gtaataaatt aaaagaagcg aacttgattg ccgtgccagg ctgttatccg
    28621 acggcggcac agctggcgct gaaaccgttg attgatgccg atcttcttga cctcaatcag
    28681 tggccggtaa tcaacgccac cagcggcgtg agcggtgcag gacgtaaggc agcgatttca
    28741 aatagttttt gtgaagttag cctgcaaccg tatggcgtct ttacccatcg ccatcaacca
    28801 gagatcgcca cacacctcgg tgctgacgtt atcttcaccc cgcatctggg caatttcccg
    28861 cgtggcattc tcgaaaccat tacctgccgc ctgaaaccgg gtgtgaccca ggcgcaagtc
    28921 gcgcaagtgt tacagcaggc gtatgcccat aaaccgctgg tgcgactgta tgacaaaggc
    28981 gttccggcgc tgaaaaatgt cgttgggctg ccattttgcg atatcgggtt tgccgttcag
    29041 ggcgaacacc tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc ggcggcacaa
    29101 gcggtacagt gcgccaatat acgtttcggc tatgcggaaa cgcagtctct tatttaaggg
    29161 tgcaatgatg aatccattaa ttataaaact gggcggcgta ctgctggata gtgaagaggc
    29221 gctggaacgt ctgtttagcg ccctggtgaa ttatcgtgag tcacatcagc gtccgctggt
    29281 gattgtgcac ggcggcggtt gcgtggtgga tgagctgatg aaagggctga atctgccggt
    29341 gaaaaagaaa aacggcctgc gggtgacgcc tgctgatcag attgacatta tcaccggagc
    29401 actggcggga acggcaaata aaaccctgct ggcatgggcg aagaaacatc agattgcggc
    29461 cgtaggtttg tttctcggtg acggcgacag cgtcaaagtg acccagcttg atgaagagtt
    29521 aggccatgtt ggactggcgc agccaggttc gcctaagctt atcaacacat tgttggagaa
    29581 cggttatctg ccggtggtca gctccattgg cgtaacagac gaagggcaac tgatgaacgt
    29641 caatgctgac caggcagcga cggcgctggc ggcaacgctg ggcgcggatc tgattttgct
    29701 ctccgacgtc agcggcattc tcgacggcaa agggcaacgc attgccgaaa tgactgccgc
    29761 gaaagcagaa caactgattg agcagggcat tattactgac ggcatgatag tgaaagtgaa
    29821 cgcggcgctg gatgctgcgc gcacgctcgg ccgtccggta gatatcgcct cctggcgtca
    29881 tgcggaacag cttccggcac tgtttaacgg tatgccgatg ggtacgcgga ttttagctta
    29941 agttttgtta gccggaggcg cagctttccg gcattgaatt tcaaaataag gaaacagagt
    30001 tatggcactt tggggcgggc gttttaccca ggcagcagat caacggttca aacaattcaa
    30061 cgactcactg cgttttgatt accgtctggc ggagcaggat attgttggct ctgtggcctg
    30121 gtccaaagcc ctggtaacgg tcggggtgtt gaccgcagaa gaacaggcgc aactggaaga
    30181 ggcgctgaac gtgctgctgg aagatgttcg cgccaggcca caacaaatcc ttgaaagcga
    30241 cgccgaagat atccatagct gggtggaagg caaactgatc gacaaagtgg gtcagttagg
    30301 caaaaaactg cacaccgggc gtagccgtaa tgatcaggta gcgactgacc tgaaactgtg
    30361 gtgcaaagat accgttagcg agttgctgac ggctaaccgg cagctgcaat cggcgctggt
    30421 ggaaaccgca caaaacaatc aggacgctgt gatgccaggt tacactcacc tgcaacgcgc
    30481 ccagccggtg acgttcgcgc actggtgcct ggcctatgtt gagatgttgg cgcgtgatga
    30541 aagccgcttg caggatgcac ttaagcgtct ggatgtcagc ccgctaggct gtggtgcgct
    30601 ggcgggaacg gcctatgaaa tcgaccgtga acaattagca ggctggctgg gatttgcctc
    30661 agcgacccgt aacagtctcg acagcgtttc tgaccgtgac cacgtgctgg aactgctgtc
    30721 tgctgccgct atcggcatgg tgcatctgtc gcgttttgct gaagatctga ttttctttaa
    30781 caccggcgaa gcggggtttg tcgagctttc tgaccgcgtg acttccggtt catcattgat
    30841 gccgcagaag aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg gtcgtgtgca
    30901 gggcgcgtta accggcatga tgatgacgct gaaaggtttg ccgctggctt acaacaaaga
    30961 tatgcaggaa gacaaagaag gtctgttcga cgcgctcgat acctggctgg actgcctgca
    31021 tatggcggcg ttggtgctgg acggcattca ggtgaaacgt ccgcgttgcc aggaagcggc
    31081 tcagcagggt tacgccaacg ccactgaact ggcggattac ctggtggcga aaggcgtacc
    31141 gttccgcgag gcgcaccata ttgttggtga agcggtggtg gaagccattc gtcagggcaa
    31201 accgctggaa gatctgccac tcagtgagtt gcagaaattc agtcaggtga ttgacgaaga
    31261 tgtctatccg attctgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa aaggcggcgt
    31321 ctcaccgcag caggtggcgc aggcaattgc ttttgcgcag gcgcggttgg ggtaagaaca
    31381 tttatatgta caaatttgag cctggcttat cgccgggctt ttttatggca aaaaaaagcg
    31441 gatcctggag atccgcaaaa gttcacgttg gctttagtta ttcgagttga gaaactctcg
    31501 aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg agcaattatc
    31561 agtcagaatg cttgataggg ataatcgttc attgctatgc tacctatcgc catgaactat
    31621 cgtggcgatg gaggatggat aatgaatatt cgtgatcttg agtacctggt ggcattggct
    31681 gaacaccgcc attttcggcg tgcggcagac tcctgccacg ttagccagcc gacgcttagc
    31741 gggcaaattc gtaagctgga agatgagctg ggcgtgatgt tgctggagcg gaccagccgt
    31801 aaagtattgt tcacccaggc gggaatgctg ctggtggatc aggcgcgtac cgtgctgcgt
    31861 gaggtgaaag tccttaaaga gatggcaagc cagcagggtg agaccatgtc cgggccgctg
    31921 catattggtt tgattcccac ggtcgggccg tacctgctac cgcatattat cccgatgctg
    31981 caccagacct ttccaaagct ggaaatgtat ctgcatgaag cacagaccca ccagttactg
    32041 gcgcaactgg acagcggcaa actcgattgc gtgatcctcg cgctggtgaa agagagcgaa
    32101 gcattcattg aagtgccgtt gtttgatgag ccaatgttgc tggctatcta tgaagatcac
    32161 ccgtgggcga accgcgaatg cgtaccgatg gccgatctgg caggggaaaa actgctgatg
    32221 ctggaagacg gtcactgttt gcgcgatcag gcaatgggtt tctgctttga agctggagcg
    32281 gatgaagata cgcatttccg tgcgaccagc ctggaaactc tgcgcaacat ggtggcggca
    32341 ggtagcggga tcactttact gcctgcgctg gctgtgccgc cggagcgcaa acgcgatggg
    32401 gttgtttatt tgccgtgtat taagccggaa ccacgccgca ctattggcct ggtttatcgt
    32461 ccgggctcac cgctgcgcag ccgctatgag cagctggcag aggccatccg cgcaagaatg
    32521 gatggccatt tcgataaagt gttaaaacag gcggtttaaa ccgtttaacg cggctacccg
    32581 ataggcttcc gccatcgtcg ggtagttaaa ggtggtgttg acgaagtact caatagtgtt
    32641 gccgccacct ttctgttcca taatcgcctg accgatatga ataatttcgg cagcacgctc
    32701 accaaagcaa tgaatgccca gaatctcttt tgtttcccga tggaacaaaa ttttcagtgt
    32761 gcccacgttc atgccgacga tttgtgcacg tgccagatgt ttgaactggg cgcggcccac
    32821 ttcatatggc actttcatcg cggtcagctg ctgttcggtt ttgcccacag agctgatttc
    32881 cgggatggtg taaatgccgg tagggatatc ttcaatcaga tgtgcggtgg cttcaccttt
    32941 caccagcgcc tgcgcggcaa tgcgcccctg gtcataggct gccgacgcca gactcggata
    33001 accaatcacg tcgcccaccg cgtaaacatg cggctgtgcg gtctgataca tgctgttgac
    33061 cttcagctgt ccgcggctgt cagtttccag cccaatgttc tgtaacgcca gcgaatcggt
    33121 attaccggtg cgaccgttgg catagagcag acagtcagct ttcagttttt tgcccgactt
    33181 cagatgcatg atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt cgttgtgacg
    33241 aatcactacg ccactgttcc agaagtgata ggagagagaa tctgacatct cttgatcgag
    33301 aaatgccagc agccgatcgc gggtgttgat cagatccact tttacatcca taccgcggaa
    33361 gatcgacgca tattcacagc cgatcactcc agcaccatag ataagtacat ggcgcggttc
    33421 gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga aatcaacatc
    33481 tgttggatga tatggacgag agccgcaggc aataacaaat ttttcagcgg ttagtgtctc
    33541 gacgctgccg tccgggcaat ccagcgccag cgtgtgctcg tcaacaaagc gagcgttccc
    33601 ctgcaatatt tcacagtgat tacgttcgta aaatccctga cgcatgcgcg tttgttgatt
    33661 aatcacgtta tcggcatggt taaggatatc ggcaaaagaa gagcggagca gtcgcgaatg
    33721 gtcgctgtaa agtgggtttt gattaaattc tataatgcgg ctgacggcgt gacggagagc
    33781 tttcgacggg atggtacccc agtgggtgca accgccgcca acattttgat aacgctcgat
    33841 aactgcgacg cgcgcacctt gcttaaccag gcccattgca gcgccttcgc cgccggggcc
    33901 ggaacctatt actatggcat cgtaatcgta ggaatgtggc atggtagggc ttacctgttc
    33961 ttatacataa aagcaacaga atggtaacat tttatcgcgg gtaagccaat tgatccccgt
    34021 catttatctg gctatatcgt gagcggcctt tgctttgtct gtttcttact tttgacctga
    34081 acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt tccgatgtac
    34141 gtaaaagaac cggccaaaga attgcagtaa atatgtttta ttgcgttacc gttcattcac
    34201 aacactggag caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa
    34261 ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct
    34321 ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg
    34381 tgaagtggcg cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt
    34441 agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg
    34501 ccaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgcacctcgg tctccacatt
    34561 tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg
    34621 cacctccgct gcgtttcgtg ctgccgttgc gcgtgaaatt cagcacttca ttgcggaact
    34681 tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga
    34741 agccatggtg acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg gcgtcgaaca
    34801 acgtcggcaa ttagaagagc gactggtact gcaactgcga atgatttcga aaggggctta
    34861 ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga
    34921 gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgctggt ggctggctta
    34981 tcgattaatg gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg
    35041 attatttccc tggtgctgac ggtttattgc ctgcatcaac gttatcttaa tcgcaccatg
    35101 ccggtaggct tgccgggtct ggctgctgcc tgttttattc tcggcgtact gctgtacagc
    35161 acggtagttc gtgcggagta tccggatatc ggctctaact tcttcccggc agtactctcc
    35221 gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag
    35281 taatcggaat catgccggat gctgatgcat ccggcatggg ttttacttcg cggtcagtaa
    35341 tacgccgcac tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcaac
    35401 cttgtgcgtc tggcttaatg tttccagatt cttgcataac gtttccggat tgcaggagat
    35461 atacagaata cgtggatacg cctgcaccat tttctcggtt tcaccgtcca gaccgctgcg
    35521 tggtgggtcg acaaaaatgg tttcgcactg ataactcttt aaatcgatcc cttgcaggcg
    35581 gttaaactcg cgtacgccat tcatcgcctg agtaaattct tctgccgcca tacgaataat
    35641 ttgtacgtta tcaatatggt tagcggcaat gttgtattgc gcggcagcga ccgacggctt
    35701 agcgatttcg gtggctaata cccgatcaaa attgcgcgcc agtgctaatg aaaagttacc
    35761 gttgccgcag tacagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca
    35821 ttccagcatc tgaatattca tcgccgcgtt cggctgggta aagctgtttt ctacctgacg
    35881 gtagatcatc tctttccctg cgaccggcag acgttcatcg atgtaatctt gatccagctc
    35941 gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg ctcgcagtgc
    36001 atcgcgcagg gcttccgctt cctgacgcca ctcatcatcc agcttcttat ggtacagcag
    36061 ggaaaccacc gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg
    36121 cagaacggga ttattacgca cacccgcaat catcgccgtc atcaactggt tgataagttc
    36181 actggcggcg gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat
    36241 gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcacgcatcc ggtaatgact
    36301 gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa
    36361 acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt
    36421 catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc
    36481 cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt
    36541 agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg
    36601 cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc tggtgggaag
    36661 tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg gctaacgtcg
    36721 catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg
    36781 gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttctgtc acagcctttt
    36841 ctgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctaac cgttttgaac
    36901 agccgcgcag cactgtgctt gcaccaacca ccgttgtgac gcgtcaggat atcgaccgct
    36961 ggcagtcgac ctcggttaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa
    37021 acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgt
    37081 tggtgttaat tgatggcgta cgcctgaatc tggcgggggt gagtggttct gccgacctta
    37141 gccagttccc tattgcgctt gtccagcgtg ttgaatatat ccgtgggccg cgctccgccg
    37201 tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg
    37261 gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat gtctccacgc
    37321 agcaacaact gggggataag acacgggtaa cgctgttggg cgattatgcc catactcatg
    37381 gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt
    37441 ttttaagtaa aacgctttat ggcgcgctgg agcataactt tactgatgcc tggagcggct
    37501 ttgtgcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat tctcctggct
    37561 caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata
    37621 acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact
    37681 acgatcccca ttatggtcgt tatgattcgt ccgcgacgct ggatgagatg aagcagtaca
    37741 ccgtccagtg ggcaaataac gttaccgttg gccacggtaa tattggtgcg ggcgttgact
    37801 ggcagaaaca gactacgacg ccgggtactg gttatgttga ggatgggtat gatcaacgta
    37861 acactggtat ctatctgacc ggattgcaac aagtcggcga ttttacgttt gaaggcgcag
    37921 cccgcagcga cgataactca cagtttggtc gtcatggaac ctggcaaacg agtgccggtt
    37981 gggaattcat cgaaggctat cgttttatcg cttcttatgg cacgtcgtac aaggcaccta
    38041 atcttgggca actgtatggt ttctacggaa atccgaatct gaatcctgag aaaagcaaac
    38101 agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc
    38161 gtaacgatgt cagtgatttg atcgattatg atgatcacac cctgaaatat tacaacgaag
    38221 ggaaagcgcg gattaagggc gtcgaggcga ccgccaattt tgataccgga ccactgacgc
    38281 atactgtgag ttatgattat gtcgatgcgc gcaatgcgat tactgacacg ccgttgttac
    38341 gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg
    38401 gtattactta tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc
    38461 aaaccgttaa aatgggtggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct
    38521 ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct
    38581 atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca
    38641 cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc
    38701 cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat
    38761 ggcgaaaaaa gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcagtgcaa
    38821 aaacgttatc cccttgcgct ggctgtggtc gcctgcaaca ctgccagtac cgtttcactt
    38881 cctgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct
    38941 gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa
    39001 cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg
    39061 ggctcggcag agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg
    39121 gatgcgctaa aacgtatctt gcgtccgtgg ttaagaatga aagagccgcc agataccgtt
    39181 gtactggggt gtacccattt ccctctacta caagaagaac tgttacaggt gctgccagag
    39241 ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgctagag
    39301 catgaagccc cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg
    39361 ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa
    39421 ctggcagttt taggctgatt tggttgaatg ttgtgcggtc agaaaattat tttaaatttc
    39481 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
    39541 acacgccgcc gggtcagcgg ggttctcctg aga
//
LOCUS       DAYPHN010000025        38992 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000025 DAYPHN010000000
VERSION     DAYPHN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.025"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..849)
                     /gene="metB"
                     /locus_tag="ACG0BW_001293"
     CDS             complement(<1..849)
                     /gene="metB"
                     /locus_tag="ACG0BW_001293"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFK7258281.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEHALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPEN"
     gene            1126..1443
                     /gene="metJ"
                     /locus_tag="ACG0BW_001294"
     CDS             1126..1443
                     /gene="metJ"
                     /locus_tag="ACG0BW_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFK7258282.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            1503..2111
                     /gene="yiiX"
                     /locus_tag="ACG0BW_001295"
     CDS             1503..2111
                     /gene="yiiX"
                     /locus_tag="ACG0BW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFK7258283.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVKQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2172..2384)
                     /gene="rpmE"
                     /locus_tag="ACG0BW_001296"
     CDS             complement(2172..2384)
                     /gene="rpmE"
                     /locus_tag="ACG0BW_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFK7258284.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            2587..4785
                     /gene="priA"
                     /locus_tag="ACG0BW_001297"
     CDS             2587..4785
                     /gene="priA"
                     /locus_tag="ACG0BW_001297"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFK7258285.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4941..5966
                     /gene="cytR"
                     /locus_tag="ACG0BW_001298"
     CDS             4941..5966
                     /gene="cytR"
                     /locus_tag="ACG0BW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFK7258286.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            6058..7017
                     /gene="ftsN"
                     /locus_tag="ACG0BW_001299"
     CDS             6058..7017
                     /gene="ftsN"
                     /locus_tag="ACG0BW_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFK7258287.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLVQQSRTTEQSWQQQTRASQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            7110..7640
                     /gene="hslV"
                     /locus_tag="ACG0BW_001300"
     CDS             7110..7640
                     /gene="hslV"
                     /locus_tag="ACG0BW_001300"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFK7258288.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            7650..8981
                     /gene="hslU"
                     /locus_tag="ACG0BW_001301"
     CDS             7650..8981
                     /gene="hslU"
                     /locus_tag="ACG0BW_001301"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFK7258289.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            9048..9974
                     /gene="menA"
                     /locus_tag="ACG0BW_001302"
     CDS             9048..9974
                     /gene="menA"
                     /locus_tag="ACG0BW_001302"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFK7258290.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            10067..10552
                     /gene="rraA"
                     /locus_tag="ACG0BW_001303"
     CDS             10067..10552
                     /gene="rraA"
                     /locus_tag="ACG0BW_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFK7258291.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            10612..11286
                     /locus_tag="ACG0BW_001304"
     CDS             10612..11286
                     /locus_tag="ACG0BW_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258292.1"
                     /translation="MQNIILLILYLFLIAVNHFRYKFLLSGNTGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSTWDYSFPGPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNEVGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            11409..12035
                     /locus_tag="ACG0BW_001305"
     CDS             11409..12035
                     /locus_tag="ACG0BW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258293.1"
                     /translation="MVGEYIGSVFLGPLLLPVLVSGILCAFAKKTRNFASFTRGCCWV
                     LGILLLSNISNTFRIITPWQYTFENAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADGSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(12074..12319)
                     /gene="zapB"
                     /locus_tag="ACG0BW_001306"
     CDS             complement(12074..12319)
                     /gene="zapB"
                     /locus_tag="ACG0BW_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFK7258294.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            12744..13589
                     /gene="glpF"
                     /locus_tag="ACG0BW_001307"
     CDS             12744..13589
                     /gene="glpF"
                     /locus_tag="ACG0BW_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFK7258295.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            13612..15120
                     /gene="glpK"
                     /locus_tag="ACG0BW_001308"
     CDS             13612..15120
                     /gene="glpK"
                     /locus_tag="ACG0BW_001308"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFK7258296.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            15255..16265
                     /gene="glpX"
                     /locus_tag="ACG0BW_001309"
     CDS             15255..16265
                     /gene="glpX"
                     /locus_tag="ACG0BW_001309"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFK7258297.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            16362..17108
                     /gene="fpr"
                     /locus_tag="ACG0BW_001310"
     CDS             16362..17108
                     /gene="fpr"
                     /locus_tag="ACG0BW_001310"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFK7258298.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETTAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(17113..17541)
                     /gene="uspD"
                     /locus_tag="ACG0BW_001311"
     CDS             complement(17113..17541)
                     /gene="uspD"
                     /locus_tag="ACG0BW_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFK7258299.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(17568..17867)
                     /gene="yiiS"
                     /locus_tag="ACG0BW_001312"
     CDS             complement(17568..17867)
                     /gene="yiiS"
                     /locus_tag="ACG0BW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFK7258300.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(18079..18519)
                     /gene="yiiR"
                     /locus_tag="ACG0BW_001313"
     CDS             complement(18079..18519)
                     /gene="yiiR"
                     /locus_tag="ACG0BW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFK7258301.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTEDVKYKADNKSSN"
     gene            18620..19219
                     /gene="yiiQ"
                     /locus_tag="ACG0BW_001314"
     CDS             18620..19219
                     /gene="yiiQ"
                     /locus_tag="ACG0BW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFK7258302.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            19327..20094
                     /gene="tpiA"
                     /locus_tag="ACG0BW_001315"
     CDS             19327..20094
                     /gene="tpiA"
                     /locus_tag="ACG0BW_001315"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFK7258303.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(20149..20904)
                     /gene="cdh"
                     /locus_tag="ACG0BW_001316"
     CDS             complement(20149..20904)
                     /gene="cdh"
                     /locus_tag="ACG0BW_001316"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFK7258304.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(21011..22000)
                     /gene="sbp"
                     /locus_tag="ACG0BW_001317"
     CDS             complement(21011..22000)
                     /gene="sbp"
                     /locus_tag="ACG0BW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFK7258305.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(22320..23282)
                     /gene="pfkA"
                     /locus_tag="ACG0BW_001318"
     CDS             complement(22320..23282)
                     /gene="pfkA"
                     /locus_tag="ACG0BW_001318"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFK7258306.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(23463..24365)
                     /gene="fieF"
                     /locus_tag="ACG0BW_001319"
     CDS             complement(23463..24365)
                     /gene="fieF"
                     /locus_tag="ACG0BW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFK7258307.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(24514..25014)
                     /gene="cpxP"
                     /locus_tag="ACG0BW_001320"
     CDS             complement(24514..25014)
                     /gene="cpxP"
                     /locus_tag="ACG0BW_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFK7258308.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            25164..25862
                     /gene="cpxR"
                     /locus_tag="ACG0BW_001321"
     CDS             25164..25862
                     /gene="cpxR"
                     /locus_tag="ACG0BW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFK7258309.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            25859..27232
                     /gene="cpxA"
                     /locus_tag="ACG0BW_001322"
     CDS             25859..27232
                     /gene="cpxA"
                     /locus_tag="ACG0BW_001322"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFK7258310.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSNTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(27337..28011)
                     /gene="yiiM"
                     /locus_tag="ACG0BW_001323"
     CDS             complement(27337..28011)
                     /gene="yiiM"
                     /locus_tag="ACG0BW_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFK7258311.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDEQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(28160..29144)
                     /gene="kdgT"
                     /locus_tag="ACG0BW_001324"
                     /pseudo
     CDS             complement(28160..29144)
                     /gene="kdgT"
                     /locus_tag="ACG0BW_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(29403..30023)
                     /gene="sodA"
                     /locus_tag="ACG0BW_001325"
     CDS             complement(29403..30023)
                     /gene="sodA"
                     /locus_tag="ACG0BW_001325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFK7258312.1"
                     /translation="MSYTLPFLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            30308..31342
                     /gene="rhaT"
                     /locus_tag="ACG0BW_001326"
     CDS             30308..31342
                     /gene="rhaT"
                     /locus_tag="ACG0BW_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFK7258313.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIFHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(31339..32187)
                     /gene="rhaR"
                     /locus_tag="ACG0BW_001327"
     CDS             complement(31339..32187)
                     /gene="rhaR"
                     /locus_tag="ACG0BW_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFK7258314.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQR
                     AIPGFSASAGQPHWRLGSMGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDEFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(32261..33097)
                     /gene="rhaS"
                     /locus_tag="ACG0BW_001328"
     CDS             complement(32261..33097)
                     /gene="rhaS"
                     /locus_tag="ACG0BW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFK7258315.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQKTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            33385..34854
                     /gene="rhaB"
                     /locus_tag="ACG0BW_001329"
     CDS             33385..34854
                     /gene="rhaB"
                     /locus_tag="ACG0BW_001329"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFK7258316.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLM
                     ADYFSYRLTGEMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLSGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            34851..36110
                     /gene="rhaA"
                     /locus_tag="ACG0BW_001330"
     CDS             34851..36110
                     /gene="rhaA"
                     /locus_tag="ACG0BW_001330"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFK7258317.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNGSELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYSARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            36193..37017
                     /gene="rhaD"
                     /locus_tag="ACG0BW_001331"
     CDS             36193..37017
                     /gene="rhaD"
                     /locus_tag="ACG0BW_001331"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFK7258318.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTHEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            37027..37341
                     /gene="rhaM"
                     /locus_tag="ACG0BW_001332"
     CDS             37027..37341
                     /gene="rhaM"
                     /locus_tag="ACG0BW_001332"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFK7258319.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(37382..38776)
                     /locus_tag="ACG0BW_001333"
     CDS             complement(37382..38776)
                     /locus_tag="ACG0BW_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFK7258320.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         9656 a        10018 c         9975 g         9343 t
ORIGIN      
        1 attttccggc agcgacgggt gatacagttt tttcaccaac ggctgggttt gcagatattt
       61 cacaatcgcc tgcgcgttgc gctgggccag ctccatacgc ggcaccagag ttcgcaaccc
      121 gcgtagcagc agatagctgt caaacgcgcc gcccgtcacg ccaatattgt ttgcccacca
      181 ggcaagttca gtgacaacgt ccggatcttt agcaatcacc acgccggcca ccacgtctga
      241 gtgaccgttc agatatttcg tgcatgaatg caacaccaga tcggcaccta atgccagcgg
      301 attttgtaat gccgggctta agaaggtgtt atccaccacg ctcaccgccc cgacttccct
      361 tgccagatgg cagattttcg caatatccac gacgcgtaac aatggattac ttgggctttc
      421 taccagtacc agtttgggtt tttctgccag cgctgcccgt aatgcatgtt catcgccttg
      481 atcaacaaac aacacgcgat agcaaccacg tttagccagg ctgtcgaaca ggcgatagct
      541 gccgccatag cagtcgtgcg gcgcaaccag cagatcgccc ggtttcaaaa agacggtcgt
      601 taccaggtga atcgcggaca tgccggtatt agtaagtacg gcaccggcac cgccttccag
      661 ttctgccagc gcacgctgaa ccacatcgcg cgttgggttg ccacgacgcg agtaatcatg
      721 cgcgcgcggt tcattaaatc cggtaaagtt ataggtgctg gaaagatgga tcggtgggac
      781 aacgcaacca tactgttcgt cgtcatttaa cccgctacgc actgcgatgg tggcctgttt
      841 acgcgtcatg tgatgaagtt ccctgggctt ggtcggtgaa atgtcaggca ccagagtaaa
      901 cattgtgtta atggacgtca atacatctgg acatctaaac ttctttgcgt atagattgag
      961 caaatcccaa atagccgtta aaattatatg cattatcacg ccgacaggtg cattacacga
     1021 tgtcacggta acgcctgtac ggtaaactat gcgggtttac ggtcagtacc cacactaact
     1081 gtgtggtctg gtctcaattt attgacgaag aggattaagt atctcatggc tgaatggagc
     1141 ggcgaatata tcagcccata cgctgagcac ggcaagaaga gtgaacaagt caaaaagatt
     1201 acggtttcca ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac gcgtcgtcag
     1261 gtgaacaacc tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt tctgcatgcc
     1321 tttaccgggc aacctttgcc ggatgatgcc gatctgcgta aagagcgcag cgacgaaatc
     1381 ccggaagcgg caaaagagat catgcgtgag atggggatta acccggagac gtgggaatac
     1441 taaagcggaa aagagcggcg cggagttaac ctgccgcttt ttttatacat ggacgtttaa
     1501 cgatgaaaat caggctactg atccccggtc tgctggtttc tgtgcctgca tttgcctggc
     1561 aaccacaaac cggcgacatc atctttcaga tctctcgctc atcgcaaagt aaagcgatcc
     1621 aactggcgac ccattccgat tacagccaca ccggtatgct ggtgatgcgt aacaaaaagc
     1681 cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggattgccc
     1741 atggtgaaaa gggcaaatac gttgttcgcc gcgttgaagg cggactgagt gttaaacaac
     1801 agcaaaaact ggcgcaaacg gcaaaacgtt atctcggcaa accgtacgat ttcagcttct
     1861 cgtggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaatgcgc
     1921 tgggaatgcg tgtgggcgaa cagcagaagc tgaaagagtt cgatcttagt aacccgctag
     1981 ttcaggcaaa gctcaaagaa cgctacggca aaaatattcc gcttgaggaa acggtggtct
     2041 caccacaagc tgttttcgat gcaccacaac tcaccactgt cgccaaagaa tggccgctgt
     2101 tttcgtggta atacgaccaa aattcaggca caaaaaaagc gccgtgcggc gcttttttcg
     2161 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
     2221 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
     2281 tcgaggttca ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag
     2341 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
     2401 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
     2461 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa
     2521 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
     2581 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttccccgtac ctttgactat
     2641 ctgctgccag aaggcatgac ggtcaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa
     2701 cagcaggagc gcatcggggt tgtggtgtca gttagcgatg tcagcgagct gccgctcaac
     2761 gagctgaaag cggtagttga agtgctggac gttgagccgg tatttactca ctccgtctgg
     2821 cgattactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     2881 gccttgccga ttttactacg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     2941 tttgccactg aacaaggtca ggcggtggat ctgaacagcc tgaaacgctc acccaagcaa
     3001 caacaggcgc tggcagcgtt acggcaaggc aaaatctggc gcgaccaggt tgccacgctc
     3061 gaatttaatg atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt
     3121 gaaacaccag agtttagtga ctggcgaaag aattatgccg tttctggtga gcggttgcga
     3181 ttgaatactg aacaggccac cgccgttggc gcaattcata gtgcggcgga taccttctct
     3241 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     3301 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgacg
     3361 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtagaagt tctgcattcc
     3421 ggcctgaacg acagcgagcg tctttcggcg tggttgaaag cgaaaaatgg tgaggcggcg
     3481 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     3541 attgatgaag agcacgacag ttcctacaag cagcaggaag gctggcgcta tcatgcccgc
     3601 gacctggcgg tttatcgtgc gcacagtgag caaatcccga ttattcttgg ctccgcaaca
     3661 cccgcactgg aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacg
     3721 cgtcgggcgg ggaatgcgcg tccggcaatt caacacgtgc tggatttaaa aggtcagaag
     3781 gtgcaggcgg ggctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     3841 aatcaggtta tcctctttct taaccgccga gggtttgcgc ctgcgctact gtgccacgac
     3901 tgtggctgga tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     3961 cagcatttgc gctgccacca ttgcgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     4021 tgcggttcca cgcacctggt gccggtgggg ctgggcaccg aacagcttga acagacgctt
     4081 gcgccgctgt tccctggtgt gcccatttct cgtatcgacc gcgataccac cagccgcaaa
     4141 ggagcgctgg aacaacaact ggcggaagtg catcgcggcg gcgcgaggat cttgatcggc
     4201 acgcaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     4261 gtggacggtg cgctgttttc tgccgatttt cgctcggcag agcgttttgc ccagctttac
     4321 acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg
     4381 catcatccag aacacccgct attgcaaacg ttgctctata aaggctacga cgcctttgcc
     4441 gaacaggcgc tggctgagcg acgcatgatg cagctgccgc catggaccag ccatgtgatt
     4501 gtgcgggcgg aagatcataa caatcagcac gcgccgctgt tcctgcaaca actgcgtaat
     4561 ctcatcctcg ccagcccgct tgcagacgac aaactgtggg ttctcggtcc cgttccggca
     4621 ctggcaccta aacgtggcgg ccgctggcgc tggcaaattt tgttgcagca cccttcccgc
     4681 gtgcgattac agcacatcat caatggtacg ctggcgctta ttaatacaat accggattcc
     4741 cgtaaggtga aatgggtgct ggatgtcgat cccatcgagg gctaatgcgc aaacgatgcg
     4801 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     4861 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     4921 gtgccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     4981 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     5041 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     5101 taccgcagcc tatgggacgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     5161 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     5221 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     5281 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     5341 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     5401 aatttgcacc ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg
     5461 atgcagtaaa ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     5521 aagagatgcc gctgtgtcac taccgcctgc aaggctacgt tcaggcgctg cgtcgctgcg
     5581 gcattatggt tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     5641 aagcaatgca acagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg
     5701 atgtgatggc gctcggcgca ctttcgcagg caaaacgcca ggggctgaaa gtcccggaag
     5761 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     5821 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     5881 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     5941 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     6001 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     6061 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     6121 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     6181 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag
     6241 tccgagacgc tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa
     6301 cgctggcgct acatcaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcctacagaa
     6361 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgccagctt
     6421 cttgagcaaa tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg
     6481 aatgaacaga cgccggaaca gcgtcagcaa acgctacagc gccagcgtca ggcgcagcaa
     6541 ttagcggagc agcaacgcct ggttcagcag tcgcgtacga ctgaacaaag ctggcagcag
     6601 cagacgcgtg cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct
     6661 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     6721 aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     6781 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     6841 gagacggtac gtgctcagct ggcgttcgaa ggctttgact caaaaattac caccaacaat
     6901 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     6961 aatcgcttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     7021 cctcaaaatc ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta
     7081 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     7141 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     7201 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     7261 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     7321 atctggtcaa agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca
     7381 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     7441 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     7501 aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa
     7561 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     7621 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     7681 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     7741 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     7801 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     7861 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct
     7921 atgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     7981 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     8041 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     8101 aaccgtccgc tgctcgtcag gcattccgca agaaactgcg tgaaggccag cttgatgaca
     8161 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccgg
     8221 gaatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     8281 aaaaagcacg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     8341 cgaaactggt gaatccggaa gagctgaaac aagacgctat cgacgctgtt gagcagcacg
     8401 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     8461 atgtttctcg tgaaggtgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     8521 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     8581 agattgcgaa accgtctgac ctgatcccgg aactgcaagg tcgtctgcca atccgcgttg
     8641 agctgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     8701 ttaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     8761 ccggtattaa gcgcatcgcg gaagcagcat ggcaggtgaa cgaatctacc gaaaacatcg
     8821 gtgctcgtcg tttacatacc gttctggagc gtttaatgga agagatttcc tacgacgcca
     8881 gcgatttaag cggtcaaacc atcactattg acgcagatta tgtgagcaaa catctggatg
     8941 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     9001 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     9061 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     9121 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcacttc gatccgctgg
     9181 tcgccctgct ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg
     9241 attacggcga tgcggtaaaa ggcagcgata aacctgaccg tatcggaccg ctacgcggca
     9301 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     9361 tcatctgtct ctccgggctg gcactggttg cagtggcgtg ccatacactg gccgattttg
     9421 tcggcttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     9481 atcgtcctta tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt
     9541 tgagcgtcat gggcagctgg tatttacagg ctcatacatt gattccggcg ctgatccttc
     9601 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa cattaataac ctgcgtgata
     9661 tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     9721 cgcgtcgtta tcatgcctgc ctgctgatgg gctctctggt gtgcctggcg ctgtttaatc
     9781 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga
     9841 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgaccg atgctggaac
     9901 gcactgtcaa gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc
     9961 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcctaca
    10021 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
    10081 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
    10141 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
    10201 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
    10261 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
    10321 tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagct
    10381 ggatatcggc atccaggcga tggcggcaat tccggttggt gctgctggcg aaggcattgg
    10441 cgaaagcgat gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta
    10501 tgccgacaat accgggatta ttctttcaga agatccgctg gatattgaat aactctctgc
    10561 catgcgttaa aaggggaatt aaattcccct tttttataca tggacgtatt catgcaaaat
    10621 attattcttt taattttata tttatttctt atagccgtta accatttccg ctacaaattt
    10681 cttttatctg gcaacaccgg tgaaatgatt ttatattttg ctaacgtatt ttttaaccca
    10741 cttgccctat catttattat cgccacaatc atctgtataa cgataaaaaa aagaagcagc
    10801 cgcagcttta ttcgcggcac ttgttggtta atgggattat ttctaatcta tagcagctat
    10861 acggtgtggg aacgacacag cacatgggat tattctttcc cggggccagg tatcaccgtc
    10921 acggttccga gcaaacagtg ggttgccaat atagtaaaac aaggaccaac cctggtgact
    10981 cgggatgccc atgctatttt agctattgtt gccttctcac aaaatgaagt cggtgtgcat
    11041 tcaatcgagg aacttaccgc gactcaaaac ggaactgcag aaatgcatgt ttgtgatgtt
    11101 cagggatttg cctgcgctta tcaggaagga gtgcaaacaa tgagtgatgg caaagtaaga
    11161 catgctattt tcatgactct actggataac actaatttga tacaactggt cgcagccatc
    11221 gacccagatt atttggacga atatcaagaa gaagtaatga aaatgatgct ttctgcccgt
    11281 caatgatctg atgtgcatgt taataataag ggattgatat gagatacgtt atcttattaa
    11341 tactatggat aataactgct tgtcttagtc atcttcagca cagcaaccta ctgtctggag
    11401 atcctggcat ggttggcgag tatatcggtt cagtatttct ggggccgtta ttacttcccg
    11461 tgcttgtgtc aggcattctg tgcgcattcg ctaaaaaaac gcgtaacttc gccagtttta
    11521 ctcgcgggtg ctgttgggta ttgggaatcc tgcttttatc caacatcagc aatacgtttc
    11581 gcataatcac cccatggcaa tatacgtttg agaatgccgc tatttccgtg actgtcccaa
    11641 accgacactg gaatacggtt tctattagta ctgataaaac cattgatata agaagtgaag
    11701 ataatagcgt ctttatttct gctttccgcc tgcccgcagg acgtagcgcc gatggctcgc
    11761 tggaagagtt aaagaagatg cagcgagaca atctcaagga tcaatacaat gaagaaacct
    11821 tccagtttca tgattgtaat gccaaacatt tcacctgtaa gtatcaggat gtgttgataa
    11881 attttgatgg tcagcagaaa agaaccatca gtgtttatct tgaagacacc cccagggctg
    11941 tggggatcat tgcattaatg gagccagata cggcagataa atatcgccaa caggcgatgg
    12001 agattatgtt gtctgctaaa aatacagtga agtaaaaaat aaggcgcctt gcggcgcctc
    12061 tgcgtggaag agattaaacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    12121 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    12181 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    12241 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    12301 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaatcctc
    12361 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
    12421 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
    12481 catccttctc ctggagacat aatccacacc aatcgaaaat gttaataaat ttgttgcgcg
    12541 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaatggcgc gataacgctc
    12601 attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
    12661 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
    12721 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
    12781 ctgaattcct cggtaccggg ttgttgattt ttttcggggt gggttgcgtt gcagcactaa
    12841 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
    12901 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaacccc gctgttacca
    12961 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
    13021 aagttgccgg cgctttctgc gctgcggctt tagtttacgg gctttactac aatttatttt
    13081 tcgacttcga gcagactcat catattgttc gcggcagcgt tgaaagtgtt gatctggctg
    13141 gcactttctc tacttacccg aatcctcata tcaattttgt gcaggctttc gcagttgaga
    13201 tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg
    13261 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    13321 ctatgggccc attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    13381 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    13441 tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    13501 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gagaccacaa
    13561 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    13621 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    13681 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    13741 ggctgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    13801 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    13861 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    13921 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    13981 tatatccgca gcaataccgg tctggtgatt gacccgtact tctctggcac caaagtgaag
    14041 tggatcctcg accatgtgga aggctctcgc gaacgtgcgc gtcgtggtga attgctgttt
    14101 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    14161 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    14221 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    14281 gtgtacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    14341 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    14401 aatacctacg gcactggctg ctttatgctg atgaacaccg gcgagaaagc ggtgaaatca
    14461 gaaaacggcc tgctgaccac catcgcctgt ggcccaactg gcgaagtaaa ctatgcgctg
    14521 gaaggtgcgg tgtttatggc gggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    14581 attaacgacg cgtacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgta
    14641 tatgtggttc cggcatttac cgggctgggt gcgccatact gggatccgta tgcgcgcggg
    14701 gcgattttcg gcttaacgcg tggagtgaac gctaaccaca ttatccgcgc gacgctggag
    14761 tctattgctt atcagacgcg tgacgtactg gaagcaatgc aggccgactc cggtattcgt
    14821 ctgcacgcgc tgcgcgtgga tggcggtgca gtagcaaaca atttcctgat gcagttccag
    14881 tccgatattc tcggtacccg cgttgagcgc ccggaagtgc gcgaagttac cgcattgggt
    14941 gcggcctatc ttgctggtct ggcggttggc ttctggcaga atctcgacga gctgcaagag
    15001 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    15061 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    15121 tgtaaatgcc ggatgaagcg tttatgccgc atccggtagt cctgaatcgt gcgggggcaa
    15181 ccccgcacac atcaataatc ccgcccttcc cctgtgctac acttcgcgcc attccttact
    15241 gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatctg
    15301 cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg
    15361 cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg
    15421 gtgaaggtga aatagacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc
    15481 gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg
    15541 gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgtttc ctcaacgcgc
    15601 cagatatgta tatggagaag ctgatcgtcg gaccgggagc caaaggcacc attgatctga
    15661 acctgccgct ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg
    15721 aactgacggt aacgattctg gctaaacctc gccacgatgc cgttatcgct gaaatgcagc
    15781 aactcggcgt acgcgtattt gctattccgg atggcgacgt tgcggcctca attctcacct
    15841 gtatgccaga cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg gaaggcgtgg
    15901 tttctgcggc ggtgatccgc gcattagatg gtgacatgaa cggtcgtctg ctggcgcgtc
    15961 atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct
    16021 gcaaagcaat gggcatcgaa gccggtaaag tattacgtct gggcgatatg gcacgcagcg
    16081 ataacgtcat cttttctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc
    16141 gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg cggcaagtca cgcactattc
    16201 gccgcattca gtccatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacattc
    16261 tctgagtgat ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg
    16321 agaacgaaga taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag
    16381 gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc
    16441 ccgtgcttcc gtttaccgcc gggcaattta ccaagcttgg ccttgaaatc gacggtgaac
    16501 gcgtccagcg cgcctactcc tatgttaact cgcccgataa tcccgatctg gagttttacc
    16561 tggtcaccgt ccccgatggc aaattaagcc cacgactggc ggcactgaaa ccgggcgatg
    16621 aagtgcaggt ggttagcgaa gcagctggct tctttgtgct ggatgaagta ccagattgcg
    16681 aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac
    16741 tgggcaaaga tttagagcgc ttcaaaaatc tggtcctggt tcacgccgca cgttatgccg
    16801 ccgacttaag ctatttgcca ctgatgcaag aactggaaaa acgctacgaa ggcaaactgc
    16861 gcattcagac ggtggtcagt cgggaaacga cagcggggtc gctcaccgga cggataccgg
    16921 cattaattga aagtggtgaa ctggaaagcg cggttggcct gccgatgaat aaagaaacca
    16981 gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacgcaacag ttactgaaag
    17041 agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt
    17101 actggtaagc ggttacttat cgataaacgg cacgatgagc aaatccgcac tcaacttatt
    17161 gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtggtgat gaccacagac
    17221 aaggaggtcg cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg
    17281 ttcaatacgc agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt
    17341 atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg
    17401 atacaactcg cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct
    17461 ggcgagctcc agggctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac
    17521 gccaatgtgt ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa
    17581 cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaggtgaa ggccgcatca
    17641 agctcaactt gcccctccag atccttaatc ttataggcca cgctggcccc ttcatctgca
    17701 gaggatgcag cggcggcaag ctctttcagt ttggtcataa aggactctgc ttcttcgcgg
    17761 gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata
    17821 tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt
    17881 atattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac
    17941 aagtaatgat cttatggatt accatttttt ttgatattgc ttaatgtgaa gagttcagaa
    18001 ggattttccg ggtattgtca tttgcatcgt ggaaataaca atcccggaaa aaaatagcag
    18061 taagagatat attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctc
    18121 ggtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag
    18181 catcatcacc agaatcaacg tcgggacaaa acgccccacc gcccattgcc agacacccgg
    18241 taaaatcgcc cagttacccg ccagcagcat ccacgccaca atcatcaaaa atgcccatgc
    18301 gccggaacgc ccgcgatcat gcaagcgctt aacggttact gccgctgttg gccagagcaa
    18361 gcacacaagg caaaacgccg cagtctgaat atcgagtaaa tttttacccg ccagtgagaa
    18421 aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat
    18481 acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc
    18541 ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc
    18601 ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc
    18661 attaaccgtt acaacaacgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta
    18721 tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa
    18781 cagccagaat cccagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa
    18841 agccaatctc actcgtgctg ccagtaaaat taacgagaac ttgtatgctt ctacagcgct
    18901 ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc
    18961 agagcaaaaa gccgcgaaag cgaaagctca ggaatacatg gcagcggtga tccgcacact
    19021 caccccatta atgaccaaaa cacaaagcca gaaaaaactg cagtcgctac taacggcggg
    19081 gaaaaacaaa cgttattaca ccgagaccga aggtgcactg cgttatgttg tcgcggacaa
    19141 cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct
    19201 tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt
    19261 tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga
    19321 attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg
    19381 gttcacgagc tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt
    19441 gcaatcgcac caccggaaat gtacatcgat atggcgaagc gtgaagctga aggcagccac
    19501 atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaacc
    19561 tctgctgcta tgctgaaaga catcggcgca cagtacatca tcatcggcca ctctgaacgt
    19621 cgtacttacc acaaagagtc tgacgaactg atcgcgaaaa aattcgcggt gctgaaagag
    19681 cagggtctga ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggcaaa
    19741 actgaagaag tttgtgcacg tcagatcgac gcggtactga aaactcaggg tgctgcagca
    19801 ttcgaaggtg cggtaatcgc ttacgaacca gtatgggcaa tcggtactgg caaatcggca
    19861 actccggcgc aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac
    19921 gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct
    19981 gcagaactgt ttgctcagcc agacatcgac ggtgcgctgg ttggcggtgc ttctctgaaa
    20041 gctgacgctt tcgcagtaat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga
    20101 aaggtgccgg atttcatatc cggcacttac tttccttaac tcctcgcctt aacgcaaaat
    20161 ctcacactga tgatcctgga tttcctcggc tgaagcacgg ttcagcgtca gtagatttcg
    20221 ttgtgtcgcc agcaatacaa atgagttatc actctgccgc accatcgcca gcccgtagct
    20281 tcccatatgt tcccgcgcct caggcacttc ctctgcaagc atcataaatg ggctgcgttg
    20341 taccagttcg ctttccgtta cccgacgcgc caggtactca tgcccacgca aaccacctgg
    20401 cagtggcaac cagcggctgc tgatgttagc cagattgtta tccagttgtt cgcgcacatc
    20461 aggacgaata caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgagagtt
    20521 gatcgccaaa gaaaccgcgc gatcgggaac cggctgaccg tattttttgc tcataaaatc
    20581 acgcgcctgc caggccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact
    20641 ttcagtaccg ttaatacgat acgttggcat caacagatat tgcagtgggc cattaagatc
    20701 ttttaaaacc acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt
    20761 ttgctgttga ttgggcaaac attcctcaag gacaatctta cgtagtgtat ccgactcttc
    20821 accggttaat ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa
    20881 aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta
    20941 acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat
    21001 cctggggaaa tcagcgtttg ctgatctgat cgaacgtacc gccgttagca aaatgctctt
    21061 tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa
    21121 acgcattttc gtactttttc gccacctcag catcgcgcgg acggtagtag tttttcgcgg
    21181 caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg
    21241 tgcctttttt ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact
    21301 cactcggcgt aacgatttcg aatttatctt tccccagctc attcgctgcc agcagagctt
    21361 cgttttccca ggcaatcagt acatcgccaa ttccgcgctc gacaaaggtg ttagtggagc
    21421 cgcgcgcgcc agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg
    21481 cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc ccaggctgcc aggtagttcc
    21541 agcgcgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca
    21601 gatcgttcca gtcatggatc tgcttcggat tgcccttacg taccaggaaa acaatggtgg
    21661 aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc
    21721 gttccgcaat cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac
    21781 cgttgattac cgacgtcgct tgtttgcctg aaccaccgtg ggactgacga atcaccacgt
    21841 tatcgccagt ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt tcgtacaatt
    21901 cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg
    21961 ccgccagcaa aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg
    22021 tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt
    22081 ggctataact tgcgggtata tgttggggga ttaaaaaggc ggaagaaatc cgcctcatat
    22141 tgctgacaaa gtgcactttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca
    22201 aaagtttgca aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca
    22261 ggcatttttg cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat
    22321 taatacagtt ttttcgcgca gtccagccag tcgcctttga acggacgctt catgttttcg
    22381 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac gcaacgaccg
    22441 ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg
    22501 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
    22561 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
    22621 atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
    22681 tcttcacggc tgaattcaac ttccggaact acaacgaatt cacagccacc ggcaatggcc
    22741 gcagccaggg tcagatcgcc acaataacga cccatcactt ccaccacgga aatacgctgg
    22801 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
    22861 aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcaggccg
    22921 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
    22981 ataaccacca gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg
    23041 ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccacc acggttgatc
    23101 atgtcagaaa cgctgtaacg gtctagctgt accatacgat cttcatacag acccagatag
    23161 ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
    23221 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc
    23281 atgactacct ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac
    23341 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
    23401 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
    23461 gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg
    23521 ataatcacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
    23581 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
    23641 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
    23701 acgatatcaa taatttcttg ccgttcctca tcaggtaatg cgcgatccag taatgactgt
    23761 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
    23821 aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgcg
    23881 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
    23941 gtccggcgca ccacccaccg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
    24001 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga
    24061 atacccgtca aaaagaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc
    24121 gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
    24181 tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
    24241 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
    24301 agtgaagcca tcgccgttgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
    24361 ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg
    24421 cattaagcag caggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag
    24481 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
    24541 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcgcgcaact gctccattcg
    24601 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
    24661 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
    24721 ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc
    24781 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
    24841 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
    24901 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
    24961 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
    25021 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
    25081 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
    25141 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
    25201 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
    25261 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa
    25321 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
    25381 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
    25441 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
    25501 cgatcctgcg ccgttcgcac tggagcgaac aacagcaaaa caacgacaac ggttcaccga
    25561 cactggaagt tgatgcctta gtgctgaatc caggccgtca ggaagccagc ttcgacgggc
    25621 aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
    25681 gtcaggtggt ttcccgtgag catttaagcc aggaagtgct gggcaaacgc ctgacgcctt
    25741 tcgaccgcgc tattgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag
    25801 atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta tctgatggtt tctgcttcat
    25861 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
    25921 gatgttagtt ttaatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
    25981 cgaacagcgt cagggtctga tgattgagca gcatgtcgaa gcggagctgg cgaacgatcc
    26041 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
    26101 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
    26161 aatgcagatc attcgtaatt ttattggtca ggccgataac gccgatcatc cgcagaagaa
    26221 aaagtatggc cgcgtggaac tggtcggtcc gttctcagtg cgtgatggcg aagataatta
    26281 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta acttactgtt
    26341 tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg
    26401 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
    26461 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
    26521 tggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
    26581 tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac
    26641 ggcgttactg cgccgtcgta gtggtgaaag caaggaactg gagcgtattg aaaccgaagc
    26701 gcaacgtctg gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcagaaaaa
    26761 cgcgctggtg agtgagacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc
    26821 ggcgttcgaa gccgagcaga tgggcaagtc gttgacggtt aacttcccgc ctgggccgtg
    26881 gccgctgtat ggcaacccga acgctctgga aagtgcgctg gaaaacattg ttcgtaatgc
    26941 cctgcgttat tccaatacga agattgaagt aggcttcgcg gtagataaag acggcatcac
    27001 cattacggtg gatgacgatg gtcctggcgt tagcccggaa gatcgcgagc agattttccg
    27061 cccgttctat cgtaccgatg aagcacgcga tcgtgaatct ggcggtacag gtttggggct
    27121 ggcgattgtt gaaaccgcca tccagcagca tcgtggctgg gtgaaggcag aagacagccc
    27181 gcttggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca
    27241 ttgtaggcag gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga
    27301 cgctgatagc gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga
    27361 aaaatcttcg atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga
    27421 taacccggcg gcggagagta aacggtgata ctgctcgtca tcaaacggca tatgccatgc
    27481 gatggcggca gcctcctgca cggtcacatc actgacacgg gaaaccaact ccagcggcgc
    27541 atcagcagat accaaccctg gcgcaatcac gctatacaac caccccactt tacccgtgtt
    27601 ttgcatcaac tgcgctatgt cgctgatatc aaaatggtaa ttgagtttat agcaagggga
    27661 gcgtggctgg ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc caatataaac
    27721 attactttcc gtgaggccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa
    27781 ctccgcctgt tccggaaatt cccgcgccca gtagagatag tgctcacgag gataatgaca
    27841 cagcgctctg tctggcccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc
    27901 cagctccgtc agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc
    27961 ttcgggataa gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct
    28021 ctccacgaga aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc
    28081 ggtatgtatc caggcaggag ctcaatagca aaccggacga acgttagttc atccggctgt
    28141 ggggattaag gaagcaggtt tattttaccg tgcctaaaat ttcgattttc gccactctgg
    28201 ctttgacttt acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga
    28261 ccgccgtcgc aaccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt
    28321 cagcaatcag cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg
    28381 cagtgccatc gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga
    28441 taattactgc cacacccaac aggatcccca gcaaacctgt ctgggcaatt acagtcaaat
    28501 caatggtatt gcccagcgcg aaggcaaaga atggaatcag cgtctgcacc gctttgctga
    28561 aaaattctcg taattcaggg tcaaggttcc caagggcaaa gcccaccagg aacggtaata
    28621 ctgcgccgac gaaaacatgc ggttcaaatg aggcaatccc ggcagtgccc agaataatca
    28681 tcgtcatgag cggcccggac tccaatgaca tcaacacaaa tgccccagct tcttcttttg
    28741 tgccgtactg ttgcatgatg gaagcgtaaa gtccgccgtt ggtcatatcc atcgccgcca
    28801 ccagcgccag cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa
    28861 tgcgtgaggc aattgccgca accacccacg cgacggcaat ttttgttacc accagcgtac
    28921 cggatttacg taataccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa
    28981 accacaccgc cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat
    29041 atttccccgc ctccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca
    29101 tcatcccccc ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg
    29161 catattttta agggctgatg cgtaattcct caacggagca tcagtgttga tggaaacgaa
    29221 gatagacatt catttaatca ataaaagtga tcaaaaccac atcaattgaa acactgtttt
    29281 atttttatcg gatcattgtt ttattttgaa attgatcaca aaaaaaccac cgccgttggc
    29341 gatggttcat tttttaagct gatatgcggc gtaaacgcct cattgcagca ggcggcaaat
    29401 gattattttt tcgccgcgaa acgtgccgct gcttcatccc agttcaccac gttccagaac
    29461 tctttgatgt agtccggacg gcggttctgg aatttcaggt agtaagcatg ttcccacaca
    29521 tccaggccca gaatcgggaa gccggaagcg ccagaaatag cttcacccat cagcggggag
    29581 tcctggttag cagtagaaac caccgccagt ttatcgcctt tcagcaccag ccatgcccag
    29641 ccggaaccaa agcgggaagc tgccgctttc tcaaattctg ctttgaagtt atcaacggag
    29701 ccgaagtcac gttcgatagc cgctttcagg tcaccctgta aggtggtgcc ttttttcaga
    29761 cctttccaga acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag tacggttttc
    29821 ttgtctgctg gcagctggtc cagtttggtg atcagctctt caaccggcag gttggcaaat
    29881 tctggcaggc tttccagcgc cgcattggcg ttgttgacgt aggtctgatg gtgtttggtg
    29941 tggtggattt ccatggtctg cttatcgaag tgcggttcca gggcatcgta agcatacggc
    30001 aggaatggca gggtatagct catattcatc tccagtattg tcgggcggcc gattgttaat
    30061 gccgcgtaag cagttggttc attatagtta attaaatgat attgaaaatg attatcaatg
    30121 ccgtactttt cgtaagggta tggttttgca ggaaaatgcc cgagatgtga agcaaatcac
    30181 ccacttaatg ccgtgattgc cagcaaatcg acaacggcgg caacaggcga aaggttaatc
    30241 gacagcacga tttttacact catctcgtcg gagatgtgac gcgacgaaaa atgatgagga
    30301 taagaagatg agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag
    30361 tgcagcctgt ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg
    30421 gtcagtcggt gggattgttt cgtggattat tctgccgtgg gccatcagcg ccctgttact
    30481 accgaatttt tgggcgtatt acagctcgtt tagtctctct acactgctgc ctgtttttct
    30541 gttcggcgct atgtggggga tcggtaatat caactacggc ctgaccatgc gttatctcgg
    30601 catgtcgatg gggattggta tcgccattgg tatcacgttg attgttggta cgctgatgac
    30661 gccaattatc aacggcaatt tcgatgtgtt gatcaatacc gaaggtggac gcatgacatt
    30721 gctcggcgtt ctggtggcgc tgattggtgt agggattgtg actcgcgccg ggcagttgaa
    30781 agagcgcaag atgggcatta aagccgaaga gttcaacctg aaaaaagggc tggtgctggc
    30841 ggtgatgtgc ggcattttct ctgccgggat gtcctttgcg atgaacgccg caaaaccgat
    30901 gcatgaagcc gctgctgcac tgggcgtcga tccactgtat gtcgctctgc caagctatgt
    30961 tgtcatcatg ggcggcggcg cgatcatcaa cctcggtttc tgcttcattc gtctggcaaa
    31021 agtgaaggat ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tctttcacaa
    31081 tgtgttactc tcagcactgg gcgggttgat gtggtatctg caattctttt tctatgcctg
    31141 gggccacgcg cgtattccgg cgcagtatga ctacatcagc tggatgctgc atatgagctt
    31201 ctatgtattg tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca atgcaggtcg
    31261 ccgtccggta acggtgttga gcctcggttg tgtggtgatt attgttgccg ccaacatcgt
    31321 cggcatgggc atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt
    31381 catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc
    31441 ggttgaaata tcactgatta acaggcggct atgctggaga agatattgcg catgacacac
    31501 tctgacctgc cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg
    31561 caaaacgcgc tcactgcacg atgcctcatc acaaaattca tccagcgcaa agggactttt
    31621 caggctggcc gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg
    31681 caacgaatca ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa
    31741 ctctgccatt tcgttagcaa acggcacatg ctgactactt tcatgctcaa gctgaccgat
    31801 aatctgccgc gcctgtgcca tccccatgct acctaagcgc cagtgtggct gccctgcgct
    31861 ggcgctaaat cccggaatcg ccctctgcca gtcaagattc agcttcagcc gctccgggca
    31921 ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc
    31981 atgaatgtaa aagagatcgc cacgggtaat gcgataaggg cgatcgttga gtacatgcag
    32041 accattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cggcaaagac
    32101 atcttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc
    32161 tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt
    32221 attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga aaaccatccc
    32281 gtccctgacg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt
    32341 ggttactgtc gctgaatccg cagcgatagg cgatgtcagt aacgctggcc tcgctgtggc
    32401 gtagcagatg tcgggctttc atcagtcgca ggcggttcag gtaacgctga ggcgtcagtc
    32461 ccgttttctg cttaagctgc cgatgtagcg tacgcagcga aagagaaaat tgatccgcca
    32521 cggcatccca attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga
    32581 gacgtgacgc gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta
    32641 attgcataaa taagatctcg cgactggcgg tcgagggtaa atcattttcc tcttcctgct
    32701 gttccatctg tgcaaccagc tgtcgcacct gctgcaacac gctgtggtta acgcgccagt
    32761 gagacggata ctgcccatcc tgctcttgtg gcagcaactg attcagcccg gcgagaaact
    32821 gaaatcgatc cggcgagcga tacagcacat tggtcagaca cagattatcg gtatgttcat
    32881 acagatgccg atcatgatcg cgtacgaaac agaccgtgcc accggtgatg gtatagggct
    32941 gcccattaaa cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat
    33001 gatgatgttc aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac
    33061 cagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac
    33121 acggcgaaat agtaaccacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg
    33181 taaaaaacgt tgatttttca agatacagcg tgaattttca ggaaatgcgg taagcatcac
    33241 atcaccacaa ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg
    33301 acccattttc ctgtcagtaa cgagaaggtc gcgaagtcag gcgcttttta gactggtcgt
    33361 aatgaaattc agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc
    33421 gcatccagtg ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg
    33481 cgtgaaatcc atcgttttaa caatgggctg catagccaga acggttatgt cacctgggat
    33541 gtggatagcc tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga ggaagggatt
    33601 cgtatcgata gcattgggat tgatacctgg ggcgtggact ttgtgctgct cgaccaacag
    33661 ggtcagcgtg tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg cctgatggcg
    33721 caggcacaac agcaactcgg caaacgcgat atttatcagc gtagcggcat acaatttcta
    33781 cccttcaata cgatctatca gttgcgtgcg ctgacggagc aacaacctga acttattcca
    33841 cacattgctc acgctctgct gatggcggat tactttagtt atcgcctgac cggcgagatg
    33901 aactgggagt acaccaacgc cacgaccacg caactggtca atatcaatag cgacgactgg
    33961 gacgagtcgc tactggcgtg gagcggtgcc aacaaagcct ggtttggtcg cccgacgcat
    34021 ccgggtaatg tcataggtca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc
    34081 gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaagcgg ttcacgcgcc
    34141 gcttatctct cttctggcac ctggtcattg atgggcttcg aaagccagac gccatttacc
    34201 aatgacacgg cgctggcagc caatatcacc aacgaaggcg gggcggaagg tcgctatcgg
    34261 gtgctgaaaa atattatggg cttatggctg cttcagcggg tgcttcagga gcggcaaatc
    34321 aacgatcttc cggcgcttat cgccgcgaca caggcactgc cggcttgccg cttcaccatc
    34381 aatcccaatg acgatcgctt tattaatcct gacgagatgt gcagcgaaat tcaggctgcg
    34441 tgtcgggaaa cggcgcaacc gatcccggaa agtgatgctg aactggcgcg ctgtattttc
    34501 gacagtctgg cgctgctgta tgccgatgtg ttgcatgaac tggcgcagct acgcggtgaa
    34561 gatttctcgc aactgcatat tgtcggcggt ggctgccaga acgcgctgct caaccagtta
    34621 tgtgctgatg cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc gacgctcggc
    34681 aatatcggca tccagttaat gacgctggat gaactcaaca atgtggatga tttccgtcag
    34741 gtcgtcagca ccaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcc
    34801 cactatgtgg cgcagattca ctctacacga cagacaaagg agctttgcgc atgaccactc
    34861 aactggaaca ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg
    34921 aggaggcgct gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg
    34981 atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg ggggattcag gctactggca
    35041 attatccggg caaagcgcgt aatggcagcg agctacgtgc cgatttggaa caagctatgc
    35101 ggctgattcc ggggccgaaa cggcttaatt tacatgccat ctatctggaa tccgatacgc
    35161 ccgtcgcgcg cgaccagatc aaaccagagc acttcaaaaa ctgggttgaa tgggcgaaag
    35221 ccaatcagct cggtctggat tttaacccct cctgtttttc gcatccgcta agcgccgatg
    35281 gctttacgct ttcccatgcc gacgacagta ttcgccagtt ctggattgat cactgcaaag
    35341 ccagccgccg tgtttcagcc tattttggcg agcaacttgg cacgccatcg gtgatgaaca
    35401 tctggatccc ggatggcatg aaagatatca ccgttgaccg tctcgctccg cgccagcgtt
    35461 tgctggcagc actggatgag gtgatcagcg agaagctgga tccggcgcac catatcgacg
    35521 ccgttgagag caaattgttt ggtattggcg cggagagcta cacggttggc tccaatgagt
    35581 tttacatggg gtatgccacc agccgccaga cggcgctgtg cctggacgcc gggcatttcc
    35641 acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgta ccgcagttgc
    35701 tactgcacgt cagccgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg
    35761 aaacccaggc gattgccagt gagattgttc gtcacgatct gtttgaccgg gtgcatatcg
    35821 gcctcgactt ctttgatgcc tctatcaacc gtattgctgc gtgggtcatt ggtacacgca
    35881 atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgagctg cgcaagctgg
    35941 aagcggcggg cgattacagt gcgcgtctgg cactgctgga agagcagaaa tcgttgccgt
    36001 ggcaggcggt gtgggaaatg tattgtcagc gtcacgatac cccagcaggt agcgaatggc
    36061 tggagagcgt gcgggcttat gaaaaagaaa ttttgagcca gcgtgggtaa acactgccgg
    36121 atgcggcgcg agcgccttat tcggccgaca gatcgtgcat ccgacaacac cgaatttaca
    36181 ggaacaccga acatgcaaaa cattactcag tcctggtttg tccagggaat gattaaagcc
    36241 accaccgacg cctggctgaa aggctgggac gagcgcaacg gcggcaacct gacgctacgc
    36301 ctggatgacg ccgatatcgc gccttatcac gacaactttc atgcacaacc gcgctatatc
    36361 ccgctcagcc agcccatgcc tttactggca aatacaccgt ttattgtcac cggctccggc
    36421 aaattcttcc gcaacgtcca gcttgatcct gcggctaact taggcgtcgt aaaagtcgac
    36481 agcgacggcg cgggctacca cattctctgg gggctaactc acgaagccgt tccaacctct
    36541 gaacttccgg cgcacttcct ctctcattgc gaacgcatta aagccaccaa cggtaaagat
    36601 cgggtgatca tgcactgcca tgccaccaac ctgatcgccc ttacctacgt actggaaaac
    36661 gacaccgcgg tcttcactcg ccaactgtgg gaaggcagca ccgaatgtct ggtagtgttc
    36721 ccggatggcg tcggcatttt gccgtggatg gtgcccggca cggacgaaat cggccaggcg
    36781 accgcacagg aaatgcaaaa acattcgctg gtgttgtggc ccttccacgg cgtcttcggc
    36841 agcggtccga cactggatga aacattcggt ttaatcgaca ccgcagaaaa atcagcacaa
    36901 gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagtcg tgaagagttg
    36961 atagcactcg gccagcgttt cggcgttaca ccactcgcca gtgcgctggc gctgtaagga
    37021 gcaaacatga tccgcaaagc ctttgtcatg caggttaacc ccgacgccca cgaagagtat
    37081 cagcgtcggc ataatccaat ctggccggaa ctggaagcag tgttgaaatc tcacggtgcg
    37141 cataactacg ccatttacct cgacaaagcg cgtaatctgc tgtttgccac ggtagagatt
    37201 gaatctgaag aacggtggaa tgcggttgcc agcactgaaa tctgccaacg ttggtggaaa
    37261 tatatgaccg acgttatgcc agctaacccg gataacagcc cagtcagtag cgagctgcaa
    37321 gaagtgtttt acctgcccta atgagttgcg gtgccggaaa atatccagca ccgcgccttc
    37381 atcagaagaa gtatttaaag cgaacgcgtg cgccgtaacg gtcatcagta tcatcaccgt
    37441 gttttttatc accgtcgcta tccagaatgg agtaatacgt cccgaggtaa atattaaagt
    37501 tctccatatc catcacatta gcgaactgat aagacgcatg aatggtgtga atggtgtacg
    37561 tcccttcatc atcaatccag cagtcgttat cacaaactac accgctgaat tcatcaatct
    37621 tattgtgcgc gtagatataa cccagctcga aacgtttcca cagcgcgtta atcccggcag
    37681 tgaaatcttt ctcattatta gcatctaaat aggcggtatt aagattaacc acgacgccat
    37741 tttccggatc cgttttcagg ccattccagg tcatgctcat accatagccg gtacgatcgg
    37801 actgatcgac aaaattaccc ttgctatcgg tataaccata agcattattt accacgttcg
    37861 cttccatcgc tgcggaaacg gtaaattctt ccgtcggcga ccaggcaatt accggacgca
    37921 gataagcaac atttttctgc tgctccatat cgcgtccatg ataattaccg tcattatata
    37981 aagatgttcc gtcttcaagt aacgtgttta attcaaaata ccagttatca agttgtttgc
    38041 tgacgagaaa attaccgcca gcgttagagc gcccgcggcc ctctttcatc atatagatat
    38101 aaccgctgcc atcgtcataa agatcgttcg cagtattacc ggaatgttca acaaaggtat
    38161 cctgattcag cgggaacata tcgtaggctt cgaaacggcc tactttgacc ttccagtcgt
    38221 tttctttgcc aaagaagaat accgcatcat cgatatttac tgaaccgtgc atgtccccca
    38281 gcggttgcgc ggagaacccg gcgaaataac cattatccag ttttcgcata ccatcaaaac
    38341 ctaacagaat acgaccattg agattccact gttcattagt gggatcgcta ttcacatcag
    38401 cgttggtcat tgccagcagg ccatgattac tttctgcatc catattaaat tctacgtcgc
    38461 cgtagatttt taaatcgcca taacctgaga gcaaaagatt aggtgaggtg gaatttttct
    38521 ttgcagattc cgccgttgcc tgtccattta ccggcgtatt gccctgggca gccttaattt
    38581 cgcggatctc agcggcttgt tgtgtctgca gctgtttaac ctggctctct gcttttgctg
    38641 cccgcatttc tgccgcttcc aggcgttctt ccagcaatgc catgcgttgt tccagcgtta
    38701 aatgctgtgt ttttgccatt ccggtactac atgccaataa taaaccgatg gctaaagata
    38761 atgtcgattt tttcattatt tacgttccct gaaataaaag agaccctacg ctttccaaaa
    38821 ggaatacgta tatagagaaa tataaggtgt gcactttcga attttcaatc caacttcagc
    38881 aggccgatgg atagtatccc gctgcataat atgagtaaat ccgctgattt cctggaacgt
    38941 gaggaatata acaatagtgc cgtttttaac caaatatcgt aaaaaatatt ag
//
LOCUS       DAYPHN010000026        38580 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000026 DAYPHN010000000
VERSION     DAYPHN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.026"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1333)
                     /locus_tag="ACG0BW_001334"
     CDS             complement(<1..1333)
                     /locus_tag="ACG0BW_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258321.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSNRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKVLACYQREANKEVALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINGLKTLHDTKIDSGTEGNDEINNIKINLSNILI
                     DSLDDAKINLEKVIDSMLETFFKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLVFDKVPLSILVNDIFLKDFFMKQPDFAKWYFYQLLKSYEGEQMYL"
     gene            complement(1456..1908)
                     /gene="tabA"
                     /locus_tag="ACG0BW_001335"
     CDS             complement(1456..1908)
                     /gene="tabA"
                     /locus_tag="ACG0BW_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFK7258322.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTPETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLRGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(2053..2646)
                     /gene="bdcR"
                     /locus_tag="ACG0BW_001336"
     CDS             complement(2053..2646)
                     /gene="bdcR"
                     /locus_tag="ACG0BW_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFK7258323.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            2717..3430
                     /gene="bdcA"
                     /locus_tag="ACG0BW_001337"
     CDS             2717..3430
                     /gene="bdcA"
                     /locus_tag="ACG0BW_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFK7258324.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3561..3956
                     /gene="yjgH"
                     /locus_tag="ACG0BW_001338"
     CDS             3561..3956
                     /gene="yjgH"
                     /locus_tag="ACG0BW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFK7258325.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4237..4371
                     /gene="pyrL"
                     /locus_tag="ACG0BW_001339"
     CDS             4237..4371
                     /gene="pyrL"
                     /locus_tag="ACG0BW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFK7258326.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4375..5310
                     /gene="pyrB"
                     /locus_tag="ACG0BW_001340"
     CDS             4375..5310
                     /gene="pyrB"
                     /locus_tag="ACG0BW_001340"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFK7258327.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5323..5784
                     /gene="pyrI"
                     /locus_tag="ACG0BW_001341"
     CDS             5323..5784
                     /gene="pyrI"
                     /locus_tag="ACG0BW_001341"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFK7258328.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAKPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5857..6243
                     /gene="ridA"
                     /locus_tag="ACG0BW_001342"
     CDS             5857..6243
                     /gene="ridA"
                     /locus_tag="ACG0BW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFK7258329.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6449..9145)
                     /gene="mgtA"
                     /locus_tag="ACG0BW_001343"
     CDS             complement(6449..9145)
                     /gene="mgtA"
                     /locus_tag="ACG0BW_001343"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFK7258330.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9286..9339)
                     /gene="mgtL"
                     /locus_tag="ACG0BW_001344"
     CDS             complement(9286..9339)
                     /gene="mgtL"
                     /locus_tag="ACG0BW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFK7258331.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9521..10471
                     /gene="treR"
                     /locus_tag="ACG0BW_001345"
                     /pseudo
     CDS             9521..10471
                     /gene="treR"
                     /locus_tag="ACG0BW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            10590..12011
                     /gene="treB"
                     /locus_tag="ACG0BW_001346"
     CDS             10590..12011
                     /gene="treB"
                     /locus_tag="ACG0BW_001346"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFK7258332.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12061..13716
                     /gene="treC"
                     /locus_tag="ACG0BW_001347"
     CDS             12061..13716
                     /gene="treC"
                     /locus_tag="ACG0BW_001347"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFK7258333.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKLRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALAGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            14110..16248
                     /gene="nrdD"
                     /locus_tag="ACG0BW_001348"
     CDS             14110..16248
                     /gene="nrdD"
                     /locus_tag="ACG0BW_001348"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFK7258334.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16406..16870
                     /gene="nrdG"
                     /locus_tag="ACG0BW_001349"
     CDS             16406..16870
                     /gene="nrdG"
                     /locus_tag="ACG0BW_001349"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFK7258335.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16915..17301)
                     /gene="cybC"
                     /locus_tag="ACG0BW_001350"
     CDS             complement(16915..17301)
                     /gene="cybC"
                     /locus_tag="ACG0BW_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFK7258336.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17484..18836)
                     /gene="pmbA"
                     /locus_tag="ACG0BW_001351"
     CDS             complement(17484..18836)
                     /gene="pmbA"
                     /locus_tag="ACG0BW_001351"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFK7258337.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18931..19482
                     /gene="yjgA"
                     /locus_tag="ACG0BW_001352"
     CDS             18931..19482
                     /gene="yjgA"
                     /locus_tag="ACG0BW_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFK7258338.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19638..21011)
                     /gene="mpl"
                     /locus_tag="ACG0BW_001353"
     CDS             complement(19638..21011)
                     /gene="mpl"
                     /locus_tag="ACG0BW_001353"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFK7258339.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21187..22185
                     /gene="fbp"
                     /locus_tag="ACG0BW_001354"
     CDS             21187..22185
                     /gene="fbp"
                     /locus_tag="ACG0BW_001354"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFK7258340.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22218..23213)
                     /gene="yjfF"
                     /locus_tag="ACG0BW_001355"
     CDS             complement(22218..23213)
                     /gene="yjfF"
                     /locus_tag="ACG0BW_001355"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFK7258341.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23200..24222)
                     /gene="ytfT"
                     /locus_tag="ACG0BW_001356"
     CDS             complement(23200..24222)
                     /gene="ytfT"
                     /locus_tag="ACG0BW_001356"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFK7258342.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24236..25738)
                     /gene="ytfR"
                     /locus_tag="ACG0BW_001357"
     CDS             complement(24236..25738)
                     /gene="ytfR"
                     /locus_tag="ACG0BW_001357"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFK7258343.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQFSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25878..26834)
                     /gene="ytfQ"
                     /locus_tag="ACG0BW_001358"
     CDS             complement(25878..26834)
                     /gene="ytfQ"
                     /locus_tag="ACG0BW_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFK7258344.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27144..27674
                     /gene="ppa"
                     /locus_tag="ACG0BW_001359"
     CDS             27144..27674
                     /gene="ppa"
                     /locus_tag="ACG0BW_001359"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFK7258345.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28054..28395)
                     /gene="ytfP"
                     /locus_tag="ACG0BW_001360"
     CDS             complement(28054..28395)
                     /gene="ytfP"
                     /locus_tag="ACG0BW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFK7258346.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28398..32177)
                     /gene="tamB"
                     /locus_tag="ACG0BW_001361"
     CDS             complement(28398..32177)
                     /gene="tamB"
                     /locus_tag="ACG0BW_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFK7258347.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWKIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVSI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLTGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVADPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32174..33907)
                     /gene="tamA"
                     /locus_tag="ACG0BW_001362"
     CDS             complement(32174..33907)
                     /gene="tamA"
                     /locus_tag="ACG0BW_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFK7258348.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34113..34751
                     /gene="msrA"
                     /locus_tag="ACG0BW_001363"
     CDS             34113..34751
                     /gene="msrA"
                     /locus_tag="ACG0BW_001363"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFK7258349.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35074..36417
                     /gene="paeA"
                     /locus_tag="ACG0BW_001364"
     CDS             35074..36417
                     /gene="paeA"
                     /locus_tag="ACG0BW_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFK7258350.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36479..36685)
                     /gene="ytfK"
                     /locus_tag="ACG0BW_001365"
     CDS             complement(36479..36685)
                     /gene="ytfK"
                     /locus_tag="ACG0BW_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFK7258351.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37010..37564
                     /gene="ytfJ"
                     /locus_tag="ACG0BW_001366"
     CDS             37010..37564
                     /gene="ytfJ"
                     /locus_tag="ACG0BW_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFK7258352.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKAAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(37654..38565)
                     /gene="ytfI"
                     /locus_tag="ACG0BW_001367"
     CDS             complement(37654..38565)
                     /gene="ytfI"
                     /locus_tag="ACG0BW_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFK7258353.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFNKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNENVHAMDKFLISFSLKDNGAEYIMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPERIKGYIRQ
                     AFGDVSLGYAVLDDIVSKLKRGKFDLQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITEEKICEKYKSTIEAFKKKQLALPV"
BASE COUNT         9434 a        10245 c         9447 g         9454 t
ORIGIN      
        1 taagatacat ctgctcgccc tcataactct taagtaattg atagaagtac cacttagcaa
       61 aatcaggttg cttcatgaaa aaatccttca aaaaaatatc attcactaaa atgctgagag
      121 gcactttatc aaaaaccaat ttaacatcat caaaaagcct tagtattaag cgcgattttt
      181 cttgattact taaattaaaa tcattgacat atacaaccaa cttctcccaa atcaattcct
      241 gtaactcagg gctgttttta tagggttctt tattgaggtt gaaaaataat gtctcagcta
      301 ttttagactg atcgcagtta aaatcaatat gatcgatcac ggagcgggca tgctttatct
      361 ttgtagtatc atcagaatat tgcatacttt gattaaaaca ccactccaga atttttacat
      421 cattaatata tggcaattta aaaaaagtct ccaacatcga atcgatgacc ttttcgagat
      481 taatttttgc atcatctaag gaatcaataa gaatattaga caggttaatt ttgatgttat
      541 taatttcatc attaccttct gtaccactat caattttggt atcatgtaac gttttcagac
      601 catttatttc ttcaagtata ccaccttcgc ctagtaagta tggaatcgtg tcatcacata
      661 catgaggcat taatagcaga tgttctttct ttattttttc taatgtactg gctaatggct
      721 tttctaataa ctcgttataa tcaattgcgt ccttccagtt caactgatga ttatttaata
      781 gaagcgccac ttccttgtta gcttcacgtt gataacacgc cagaaccttg ttagtaatca
      841 ggtgtaatga actctcatct tttctttcaa aggactttcc ttcataacga ttagtcgaac
      901 catcaggttg ttgaaatata atagccgtat gtccttgacg agtccccata gtagtgatag
      961 tggaaagtgt aatttctatt tccaccttag ataaatccgc tcgctcccct tcaggattat
     1021 tcataacaaa cttcattatc tcactgccat cattattttt gatataagat gaggactcaa
     1081 tatgagccat catatttctt cccgcttcgt tggagcggtt gctaaaatcc acataaccca
     1141 gggtaaatat ttttaccaaa aactctaaaa atgtcttagg atcgaggata tttcttgtat
     1201 tatcctgccc ctcggcatta cgagcaaaat tattcattcc aacacgccag gtatttaaaa
     1261 cttcactttt ttgtttaaaa ttgtcggcta atgtaatgtg ttgagaataa ttcaaatctg
     1321 atattttgct catgacggac tccttgagtt aataagaacg tctaagcatt acaaatttta
     1381 ctcacaaaaa aatatcaaaa aacgctcttt tcacaaactc gccgtaaacc tggtgttatt
     1441 tacggcgaaa agtcatcacg ccatcagcat cttcactact gctttgcgaa cctgggctgg
     1501 tgcgcccact gcgcacagcg gtttatgcac ttcccccgga taaaacacaa caaaatcacc
     1561 ttcatttagg ataactgttt tctcatcaac gccttccggc aaaaatgcga tgtctttatc
     1621 agctaaccag tcggtatccg gcgtgcctgc aggttgcgtg ctgaaggtca tgccttcctg
     1681 acctcttaac acaatctgaa tgtcgagata gcgggcatgg tactccgcac ggcgagcttc
     1741 gtacggctcg gtcatatctt ccgagataag ataaaacaga cgattgcctt cgatatcgtg
     1801 cttacccttt ggcgtttctg gcgtaacgtg cgctttgata tgctcaatcg cctggcgtaa
     1861 ctcctggggt agccacggct gaagattatg aatatttcca atgatcatct tttccactct
     1921 ttaattaaaa caatgtttca tttctatcgt tatacgaaaa gatgatcaat tccgctagcc
     1981 ttaacgcgtg aattattgtc ttatcacaac ttatcggcag gcgcgttccc gcctttcggg
     2041 tcaaggccag catcactcct tgagaatagt ttttatcgct tcccccgcca gtgcagctgt
     2101 tgcacagagt tgttcgatgg agtgcccctc ccgtgccttc gcagaaagcc ctgacatcac
     2161 ggtactcata aaatcagtca cacattgtgc gctttgtgga tgccgcctgg cgatatagtc
     2221 ataaatggtc gtttctgcgg cgtgataata ttgaacggca atatcacgcg cttgtggatc
     2281 atgactatga ataccttcaa gaaccataca gccagcgcag ccgccgtttt ggctatatct
     2341 gcgcgccgct tcttttaata cctcaaccag gcactcgcct accggacgat catcacgaag
     2401 aatatcggca agcggaatag cttccgtacc gacatattca ttgagtacac ggctaaataa
     2461 cccaacttta ctgccaaaag ctgcgtagag gctcgggggg ttaataccaa gataatcagt
     2521 aacttcagca acactgacag catcgaaacc tttttggtga aaaagcactt ttgccgcaga
     2581 gactgcctgc tcaggagcga aacgtctggg acgacctgga acgcgagatt gttttttagt
     2641 gaccattaca aaacttgttg acagaaagtt aaaacagttt tgtaatgcat gttacataat
     2701 aaatcaagga gtccttatag gcgcttttac aggtaagaca gttctcatcc tcggtggcag
     2761 tcgtggaatc ggtgccgcta tcgtacgtcg tttcgtcact gatggggcca atgtacgatt
     2821 cacctatgcg gggtcgaaag atgccgctga acatctggca caagagactg gagcgacagc
     2881 agtatttaca gatagtgctg acagagacgc tgtcattgat gtcgttcgta agagcggcgc
     2941 attggatatc ctggtggtaa atgcaggtat tggcgtcttt ggcgatgccc tggaattaaa
     3001 tgccgacgat attgatcgcc ttttcaaaat caatattcat gctccttatc atgcctctgt
     3061 tgaagccgcc cggcagatgc ccgaaggcgg gcgcatctta atcatcggct ccgtgaatgg
     3121 cgatcgtatg cctgttgcag gcatggctgc ttatgccgcc agcaaatctg ccctgcaagg
     3181 catggcgcgc gggctggccc gtgattttgg accgcgtggg atcaccatta acgtcgtcca
     3241 gccagggcca attgataccg acgctaatcc cgccaacggg ccaatgcgcg atatgttgca
     3301 tagtttgatg gctatcaaaa gacatgggca accggaagag gtcgctggta tggtcgcatg
     3361 gttagcaggg ccagaagcca gttttgttac cggcgcgatg cataccattg atggcgcgtt
     3421 tggcgcataa ccgactacgc tcaattaagc ccagccatta tccatgatgt ctgggttttg
     3481 tttactcacg tcgtccgcta aaagcggctc ccggtaaaga gaaatcttct gttcatgtcg
     3541 taaatcttga ggataaccaa atggtagaaa gaaccgctgt tttccctgct ggccgacatt
     3601 cactgtatgc tgagcatcgt tattctgcgg ctattcgttc cggcgatttg ctgtttgttt
     3661 ccgggcaagt aggaagtcga gaggacggaa caccagaacc cgattttcag caacaagtca
     3721 gactggcatt tgataatttg catgcgaccc tggctgctgc gggatgcact tttgacgata
     3781 tcattgatgt tacgagcttc cataccgatc cagaaaacca atttgaagac atcatgacgg
     3841 tgaaaaatga aatatttagc gccccacctt atccaaactg gacggcggtg ggtgttacat
     3901 ggctggcagg ctttgatttt gaaattaaag tgatagcgcg catccctgag cagtaagcaa
     3961 gagtgttagc cgttcgcttt cacactccgc cctataagtc ggatgaatgg aataaaatgc
     4021 atatctgatt gcgtgaaagt gaaaaaggaa aaagcaggga atgtctgcaa ttattgatac
     4081 cgaaggacag ttcccctgca gaatcacatc aaataaaaat gcatatacct tgacttttaa
     4141 ttcaaataaa ccgtttgcgc tgacaaaata ttgcatcaaa tgcttgcgcc gcttctgacg
     4201 atgagtataa tgccggacaa tttgccggga ggatgtatgg ttcagtgtgt tcgacatttt
     4261 gtcttaccgc gtctgaaaaa agacgctggc ctgccgtttt tcttcccgtt gatcacccat
     4321 tcccagcccc tcaatcgagg ggcttttttt tgcccaggcg tcaggagata aaagatggct
     4381 aatccgctat atcagaaaca tatcatttcc ataaacgacc ttagtcgcga tgaccttaat
     4441 ctggtgctgg cgacagcggc gaaactgaaa gcaaacccgc aaccagagct gttgaagcac
     4501 aaagtcattg ccagctgttt cttcgaagcc tctacccgta cccgcctctc tttcgaaact
     4561 tccatgcacc gcctgggtgc cagcgtggtg ggcttctccg acagcgccaa tacatcactg
     4621 ggtaaaaagg gcgaaacgct ggccgatacc atttcggtta tcagcactta cgttgatgcg
     4681 atagtgatgc gtcatccgca ggaaggtgcg gcgcgcctgg ccaccgaatt ttctggcaat
     4741 gtaccggtac tgaatgccgg tgatggctcc aaccaacatc cgacgcaaac cttgctggac
     4801 ttattcacca ttcaggaaac ccaggggcgt ctggacaatc tccacgtcgc aatggttggt
     4861 gacctgaaat atggccgcac cgttcactcc ctgactcagg cgttagcgaa gttcgacggc
     4921 aaccgttttt acttcatcgc gccggacgcg ctggcaatgc cgcaatacat ccttgatatg
     4981 ctcgatgaaa aagggatcgc ctggagtctg cacagctcta ttgaagaagt gatggcggaa
     5041 gtagacatcc tgtacatgac ccgcgtgcaa aaagagcgtc tggacccgtc cgagtacgcc
     5101 aacgtgaaag cgcagtttgt tcttcgcgcc agcgatctcc acaacgccaa agccaatatg
     5161 aaagtgctgc atccgctgcc gcgtgttgat gagattgcga cggatgttga taaaacgcca
     5221 cacgcctggt acttccagca ggcaggcaac gggattttcg ctcgccaggc gttactggca
     5281 ctggttctga atcgcgatct ggtactgtaa ggggaaatag agatgacaca cgataataaa
     5341 ttgcaggttg aagctattaa acgcggcacg gtaattgacc atatccccgc ccagatcggt
     5401 tttaagctgt tgagtctgtt caagctgacc gaaacggatc agcgcatcac cattggtctg
     5461 aacctgcctt ctggcgagat gggccgcaaa gatctgatca aaatcgaaaa tacctttttg
     5521 agtgaagatc aggtagatca actggcattg tatgcgccgc aagccacggt taaccgtatc
     5581 gacaactatg aagtggtggg taaatcgcgc cccagtctgc cggagcgcat cgacaatgtg
     5641 ctggtctgcc cgaacagcaa ctgtatcagc catgccaaac cggtttcttc cagctttgcc
     5701 gtgcgaaaac gcgccaatga catcgcgctc aaatgcaaat actgtgaaaa agagttttcc
     5761 cataatgtgg tgctggccaa ttaattgcgg ttggtaataa aagtctggct ccctataatg
     5821 agccagactt tttaccgctg taataaagga gaaatcatga gcaaaactat cgcgacggaa
     5881 aatgcaccgg cagctatcgg tccttacgta cagggcgttg atctgggcaa tatgatcatc
     5941 acctccggcc agatcccggt aaatccgaaa acgggcgaag taccggcaga cgtcgctgca
     6001 caggcacgtc agtcgctgga taacgtaaaa gcgatcgtcg aagccgcagg cctgaaagtg
     6061 ggcgacatcg ttaaaaccac cgtgtttgtg aaagatctga acgacttcgc aaccgttaat
     6121 gctacctacg aagccttctt caccgaacac aatgccacct tcccggcacg ttcttgcgtt
     6181 gaagttgccc gtctgccgaa agacgtaaaa attgagatcg aagcgatcgc tgttcgtcgc
     6241 taatcttgat ggaaatccgg gctatcatgc ccggattaaa tctgatgaca aacgcaaaat
     6301 tgcctgatgc gctacgctta tcaggcctac gtgattcctg caattgattg aatttgtagg
     6361 ccggataagg catttacgcc gcatccggca tgaacaacgc tcactttgtc tgcaatctga
     6421 atcggggcta tcgtgcccag tttattcttt actgccagcc gtaacgacgg ctatagaacc
     6481 ctttcaccaa ctgggttaac gtcatatacc ctgccagaat cgcaaccagc cacgggaaat
     6541 agcttaacgg cagcgcctgc agctgcagat aactggccag cggtgaaaac ggcaatgcga
     6601 tcccgacaat catcacgatc acggtcatga tcattaacgg ccacgatgca cagctctgaa
     6661 taaacggcac acggcgggta cggatcatat gcacaatcag cgtttgcgac agtaagccca
     6721 ccacaaacca tcccgactgg aacagcgttt gcgtttccgg cgtgttggca tggaataccc
     6781 accacatcag gcaaaacgtc agaatatcga agatcgagct gatcggtccg aagaagatca
     6841 taaagcgccc cagatccgcc ggattccaac gctgcggctt ttgaatttgc tcgtcgtcga
     6901 cgttatcaaa cgggatcgcc acctgtgaca catcgtacag caggttctga ataagcaagt
     6961 gtaacggcaa catcggcagg aagggcaaga aagcactcgc caccagcacg ctgaacacat
     7021 taccgaagtt agagctcgcc gtcattttga tgtatttcag catgttggcg aaagtgcgac
     7081 gtccctcaat aaccccctct tccagcacca tcaggctttt ttccagcagg atgatatcag
     7141 ccgcttcacg ggcgatatct accgcgccgt ccacagaaat gccgatatcc gcagcgcgta
     7201 aggcgggcgc atcattaata ccatcgccca taaagccaac cacatgccct tcgcgcttca
     7261 gtaaggtcac aatgcgttct ttatgcatcg gcgtcagacg agcaaacagc gtggtgcgct
     7321 gtgcgagatt agccagttcg tcgtcagata gcgtttcaat atcactacca atgaccacct
     7381 ctcccgcgtc cagccccact tcatggcaca ctttcgctgc aactaactca ctgtcgccag
     7441 taaggatttt tacggtaatt ccactcgctt ttaatgcctt cagtgccgga gcagttgtct
     7501 cttttggcgg atcaaggaaa gcaatatatc cttcgaggat caggtcggat tcatccgccc
     7561 gctggtaatc tccttcacgc gctggcaggt atttcgtcgc caccgcaacc acgcgcagcc
     7621 cctgacgatt cagcgtatca gtaacccgct taatcttacg cagcatgatg tcatcgagcg
     7681 gcacaatctc gccattgtga cgcacctgcg aacacacatt gaggatttcc tgcaatgcac
     7741 ctttgcaaac cagctgatgg tgttcggtat tttctgccac taccaccgac atccgacgac
     7801 gctcgaaatc aaacggaatc tcatcaattt tttgccaacg gctggccagc gagcgcgctg
     7861 actcttcatc cgtaccttcg agcaccgctg tatcgagcag gtttttaagt ccggtctgat
     7921 aatgactgtt caaccacgcg ctatgcagca cgcgttcgct ggttttaccg gagatatcgg
     7981 tatgattctc cagcacaatt ttgtcctgtg tcagggtgcc ggttttatca gtgcacagaa
     8041 tatccattgc gccaaagttc tgaatagcat ccagatgttt gacgatcact ttctgtttcg
     8101 acagctttac tgcgccgcgc gccagcgtcg aggtaacaat catcggcaac atttccggcg
     8161 ttaggcctac cgcgaccgaa agcgcaaaca gcgccgcttc ccaccagtcg cctttggtgt
     8221 agccattgat taacagcacc accggtgcca tcaccagcat aaagcgaatc agcagcatac
     8281 tgacgcggct gatcccttgc tgaaaggcat tcggctcgct ttcctgctca ctaacacgcc
     8341 ccgccagttg accaaaccag gtattggcac ctgtagcaat caccattgct tgtgccgtgc
     8401 cgctcaccac ggtggtgccc ataaaacaca gggtgtcgca ctccagcgga ttgctgtgct
     8461 ccggctggcg agtggttgcg gctttttcta cgggcagaga ctcaccggtt aacgacgcct
     8521 gagcgacgaa cagatcccgc gcctgcaaga tacgtaaatc tgccgggatc atatctcccg
     8581 ccgccagttt tataatatcg ccgggcacca gctggtcgat cgggatctcc agccagccat
     8641 tttcgccttt gtcgttaatt acgcgcagca ccgtcgcagt attgctgacc atcgctttca
     8701 gggcatctgc cgctttagtg gaacgtgctt cctgaataaa gttcagcaac gtagaaatgg
     8761 cgaccattag cgcgataacg cccgcggcaa ataaatcttc cgtggcgtaa gaaatagcgc
     8821 cgagaatggt gagtaaaata ttaaaggggt tgcgatagca gacccataaa tgtacccacc
     8881 acggcgacgg ttgttgtgcg ggtaatttat tttcaccatg ttgttcgcgg gcagattcca
     8941 cttccgcctg atttaaccct tccggatggg tgtcgaacgt tttccacaat tcttcttcgg
     9001 gcatcaccgc cattttcagg caatgcgcac ttaaggacgg cgggaccacc gtattcactg
     9061 tctgctgcgc gccaggcaat ggatcacgat gaaccagacg ggaaggtaaa tggcgaatga
     9121 gccgggtaaa aatttcttta aacataagga gtccctccgc actgtctgaa acagtacaga
     9181 aatgtcttca ggcgtggcga agccttacct gaaggtaata aagctgagca gggacgttaa
     9241 aacgtcacgg ccttacgcat ccggtaacgc cgtgaaaaga cgggcttacc gaaaaagttt
     9301 taatctccgt cgagggagag gcgtgggatc aggttccata taacctccgg taagtaaaaa
     9361 aagcgccgca aataataagg agaatccaat aatattcgta gcatttatgg cgatataata
     9421 cctgctggca actaaaccaa cgataaacca gactttaccc attgctgaat gcacgggtaa
     9481 agttagcctc aaataattaa acaacacgtt acgggacaac aggatgcaaa atcggctgac
     9541 catcaaagac atcgcgcgct taagcggcgt ggggaaatct acggtttccc gggtgctgaa
     9601 taacgaaagc ggcgtgagcc agcgcacgcg cgagcgcgtg gaagcagtga tgaatcagca
     9661 tggattttcc ccttcccgct ctgcgcgcgc tatgcgtggg caaagcgata aagtggtcgc
     9721 catcattgtt acccgcctgg attcgttgtc agaaaatctc gccgttcaaa ccatgctgcc
     9781 agcgttctat gaacaaggtt acgacccaat catgatggaa agtcagtttt ccccgcaatt
     9841 agttgccgaa catttgggga tcctaaaacg gcgtaatatc gacggcgtag tgctgttcgg
     9901 ttttaccggc ataacagaag aaatgttagc ccactggcag tcatcgctgg ttctgctggc
     9961 gcgtgacgca aaaggctttg cttcggtctg ttatgacgac gaaggggcaa tcaagatcct
    10021 gatgcaacgg ctctatgacc aggggcatcg taatatcagt tatctcggcg tgccgcacag
    10081 tgacgtgacg accggtaagc gacgtcacga agcctacctg gcgttctgca aagcgcataa
    10141 actgcatccc gttgccgctc tgccagggct tgctatgaag caaggctatg agaacgtagc
    10201 aaaagtgatt acgcctgaaa ctaccgcctt actgtgcgca accgacacgc tggcacttgg
    10261 cgcaagtaaa tacctgcaag agcaacgcat cgacaccttg caactggcga gcgtcggtaa
    10321 tacgccgtta atgaaattcc ttcatccgga gatcgtcacc gtagatcctg gttacgccga
    10381 agctggacgc caggcggcct gccaattgat cgcacaggta accgggcgca gcgaaccgca
    10441 acaaatcatc atccccgcca ccctgtcctg atcgtttcct gaacgataaa ttgtgatctt
    10501 cgctgcgttt cgggaacgtt cccgttttta aatttttccg cgcaatatat tctgcagcca
    10561 accaaaaatg tcatctgcca tggggcttta tgatgagcaa aataaaccaa acggatatcg
    10621 atcggttgat tgaactggtc ggcgggcgcg gcaatattgc gacggtgagc cactgtatta
    10681 ctcgcctgcg ctttgtcctc aaccaaccgg ccaatgccag accgaaagaa attgagcaac
    10741 tccccatggt gaaaggctgt ttcaccaatg ccgggcaatt tcaggtggtg attggcacca
    10801 atgtgggtga ttactatcaa gcactgattg cgtcaaccgg acaggcgcag gttgataaag
    10861 agcaggtaaa aaaagccgcc cggcagaata tgaaatggca tgagcagttg atctctcatt
    10921 tcgcggagat cttcttcccg ttgctgcccg cgttgattag cggcggtttg atcctcggtt
    10981 ttcgcaatgt gatcggcgat ttgcccatga gcaacggtca aacgctggcg caaatgtacc
    11041 cttccctgca aacgatctac gattttctgt ggttgatcgg tgaagcgatc ttcttctacc
    11101 tgccggtcgg gatttgctgg tcagcggtga aaaaaatggg cggcacgccg atccttggta
    11161 tcgtacttgg cgtaacgctg gtttctccac agctgatgaa cgcttatctg ctcgggcagc
    11221 agctgccgga agtgtgggac tttggcatgt tcagcatcgc caaagtaggc tatcaggcgc
    11281 aggtgatccc ggcactgtta gccgggctgg cgctgggcgt tattgaaact cgccttaaac
    11341 gcatcgtgcc ggattacctc tatctggtgg tggtgcccgt ctgttcgctg atcctcgcgg
    11401 tgttcctcgc ccatgcgctg attggtccgt ttggacgcat gattggcgat ggcgttgcct
    11461 ttgcggtacg tcacctgatg accggcagct ttgcacctat tggcgcagca ttgtttggct
    11521 tcctgtacgc cccgctggtg atcaccggtg tacaccagac cacgcttgct attgatttgc
    11581 agatgattca aagcatgggt ggtacgccag tgtggccgct gattgcgctg tcgaatatcg
    11641 ctcagggctc cgccgtgata ggcattatca tttccagccg caagcacaat gaacgcgaga
    11701 tctccgtgcc tgccgctatc tccgcctggc ttggggtcac tgagcctgca atgtacggca
    11761 tcaacctgaa atatcgcttc ccgatgctgt gcgcgatgat tggttctggt ctggcaggat
    11821 tgctatgcgg cctgaacggc gttatggcga atggcatcgg cgtaggcggc ctgccgggaa
    11881 ttctctcgat tcaaccgagc tactggcagg tgtttgcgct ggcaatggct atcgccatca
    11941 tcatcccgat tgtactcacc tcgtttatct atcagcggaa ataccgcctg ggcacgctgg
    12001 acattgttta attttcttcg gggcgcaatt gcgccccctc gcattcgcag gaataacgta
    12061 atgactaatc ttccccactg gtggcaaaac ggcgttatct accagattta tccaaagagt
    12121 tttcaggaca ccacgggtag cggtaccggc gatttacgtg gcgttatcca acgcctggac
    12181 tatctgcata aactgggcgt tgatgccatc tggctaaccc ccttttatgt ctctccccag
    12241 gtcgataacg gttacgacgt agcgaactat acggcgattg atcccaccta cggcacgctg
    12301 gacgattttg acgaactggt gacgcaggca aaattgcgcg ggattcgtat cattctcgat
    12361 atggtgttta accatacctc tacccaacat gcctggtttc gcgaggcgct gaacaaagaa
    12421 agcccttacc gccagtttta tatctggcgc gatggcgaac cagaaacgcc accgaataac
    12481 tggcgttcaa aatttggcgg tagtgcgtgg cgctggcatg cggaaagcga acagtactat
    12541 ttgcatctct ttgcaccaga acaggcggat ctcaactggg agaatccagc ggtacgcgca
    12601 gagctgaaaa aagtctgtga attctgggcc gatcgtgggg tcgatgggtt gcgcctggat
    12661 gtggtgaatc tgatctccaa agacccgcgt ttccctgaag acctggatgg cgacgggcgt
    12721 cgcttctaca ccgacgggcc acgagcacac gagtttttgc acgagatgaa ccgcgatgtg
    12781 tttacgccac gcgggttaat gaccgtaggt gaaatgtcct ccaccagcct tgagcattgc
    12841 cagcgatacg cggcactggc aggcagtgaa ttatcgatga cctttaattt tcatcacctg
    12901 aaggtcgatt atcccggtgg tgaaaaatgg acgctggcta aacctgactt tgtggcgttg
    12961 aaaacattgt tccgccactg gcaacaagga atgcacaacg tagcatggaa tgccttgttc
    13021 tggtgtaacc acgatcagcc gcgcattgtt tctcgctttg gtgatgaagg tgaataccgc
    13081 gtgcctgcgg caaaaatgct agcgatggtg ctgcatggca tgcagggaac gccgtatatc
    13141 taccagggcg aagagattgg catgaccaac ccgcatttca cgcgcattac tgactatcgc
    13201 gacgtggaga gcctcaatat gtttgccgag ctgcgcaacg atgggcgtga tgccgacgag
    13261 ttattggcaa tcctcgccag taaatcccgt gacaacagcc gcacgcccat gcaatggagc
    13321 aacggcgata atgccgggtt tacggctggc gaaccgtgga ttggcctggg cgataactat
    13381 caacaaatca acgtagaagc cgcgctggcc gatgattcct cggtgtttta cacctaccaa
    13441 aagttaatcg cactgcgtaa gcaggaagcc atcctgacat ggggcaatta ccaggatctg
    13501 ctgccaaaca gccctgtatt gtggtgctat cgccgtgaat ggaaggggca aaccttgctg
    13561 gtcattgcca accttagccg tgagatccaa ccctggcagc cagggcaaat gcgcggcaac
    13621 tggcagcttg tgatgcataa ctacgaagaa gcctcaccac aaccctgtgc catgaattta
    13681 cggccttttg aggctgtctg gtggttacag aagtaaatct tccctaagcc ccggtaatgc
    13741 cggtcattcc ggggttttgt ctgtcagcat gttgtttttg ttgatttttc aaccagcaaa
    13801 ttcattaaaa aatttacata acgctgtagc gcccgtcatc cgtacgctct gctttttact
    13861 ttgagctaca tcaaaaaaag ttcaaacatc cttgatgcaa agcactatat atagacttta
    13921 aaatgcgtcc caacccaata tgttgtatta atcgactata attgctacta cagctcccca
    13981 cgaaaaaggt gcggcgttgt ggataagcgg atggcgattg cggaaagcac cggaaaacga
    14041 aacgaaaaaa ccggaaaacg cctttcccaa tttctgtgga taacctgttc ttaaaaatat
    14101 ggagcgatca tgacaccgca tgtgatgaaa cgagacggct gcaaagtgcc gtttaaatca
    14161 gagcgcatca aagaagcgat tctgcgtgca gctaaagcag cggaagtcga tgatgccgat
    14221 tattgcgcca ctgttgccgc ggttgtcagc gagcagatgc agggccgcaa ccaggtggat
    14281 atcaatgaga tccagaccgc agttgaaaac cagctgatgt cgggtccata caaacaactg
    14341 gctcgtgctt acatcgagta ccgtcacgat cgcgacattg aacgtgaaaa acgcggtcgc
    14401 ctgaaccagg agatccgtgg tctggtcgag cagaccaacg cctcgttact caacgaaaac
    14461 gccaacaaag acagcaaggt gattccaacc cagcgcgacc tgctggccgg gatcgtggct
    14521 aaacactatg cacgtcagca cctgctgccg cgtgacgtgg tgcaggcaca tgagcgtggc
    14581 gatattcact atcacgatct cgattactca ccgtttttcc cgatgttcaa ctgcatgttg
    14641 atcgacctga aaggcatgct gacccagggc tttaaaatgg ggaacgccga gattgaaccg
    14701 ccgaagtcga tctcaacggc aaccgcggta actgcgcaga ttattgctca ggttgccagc
    14761 catatttatg gcggcaccac cattaaccgt atcgatgaag tgctggcacc gtttgtcacc
    14821 gccagctaca acaaacatcg caagaccgca gaagagtgga gcatcccgga cgcagaaggc
    14881 tacgctaact ctcgtaccat taaagagtgc tacgatgcct tccagtcgct ggagtacgaa
    14941 gtaaacaccc tgcataccgc caacggtcaa acgccgtttg taacttttgg ttttggtctg
    15001 ggcaccagct gggaatcgcg cctgattcag gaatctatcc tgcgtaaccg tatcgcaggt
    15061 ctcggtaaaa accgtaaaac tgcggtgttc ccgaaactgg tgtttgcgat tcgcgatggt
    15121 ctgaaccata aaaaaggcga tccgaactac gacatcaaac agctggcgct ggagtgcgca
    15181 agcaagcgca tgtatccgga tatcctgaac tacgatcagg tagtgaaagt caccggttcg
    15241 tttaaaaccc cgatgggctg ccgcagcttc ctcggcgtgt gggaaaatga aaacggcgag
    15301 cagatccacg atggtcgtaa caacctcggc gtgatcagcc tgaacctgcc gcgtattgct
    15361 ctggaagcaa aaggcgatga agccaccttc tggaagctgc tggatgaacg tctggtgctg
    15421 gcacgtaagg cgctgatgac ccgtatcgct cgtctcgaag gcgtgaaagc gcgcgtggcc
    15481 ccgatcctct atatggaagg tgcttgtggc gtgcgtctca atgctgacga tgatgtttct
    15541 gaaatcttca aaaacggtcg tgcgtctatt tcgctgggtt acatcggcat ccacgaaacc
    15601 attaacgcgc tgttcggcgg cgagcatgtt tacgacaacg agcagcttcg cgcgaaaggt
    15661 atcgccattg ttgaacgtct gcgtcaggca gtggatcagt ggaaagaaga aacgggttat
    15721 ggtttcagtc tctacagcac gccgagtgaa aacctgtgcg accgcttctg ccgtctcgat
    15781 actgctgagt ttggcgtggt gccgggcgtg accgataaag gttactacac caacagtttc
    15841 cacctcgatg tggagaagaa ggtgaacccg tacgacaaga tcgactttga agcgccttac
    15901 ccgccgctgg cgaacggtgg tttcatttgc tacggcgagt atccaaacat tcagcacaac
    15961 ctgaaggcgc tggaagatgt ctgggattac agctatcagc atgtaccgta ttacggcacc
    16021 aatacaccga ttgatgagtg ctacgagtgt ggctttaccg gtgagttcga gtgcaccagc
    16081 aaaggcttca cttgcccgaa atgtggtaac catgacgcct cccgtgtgtc ggtaactcgc
    16141 cgcgtgtgcg gatatttagg tagcccggat gcacgtccat ttaacgccgg taagcaggaa
    16201 gaagttaagc gccgcgttaa acatttggga aatgggcaga taggttaatc ttcactctac
    16261 gttaagcaaa tgccggatgc ggcgtaaacg ccttatccgg cctacatttg gcaactgctc
    16321 caggcctgat aagacgcgac agcgtcgcat caggcattga ttaccggatg cagcctacac
    16381 gcggactgat aagccaggcg aaataatgaa ttatcatcag tactatcctg tcgacatcgt
    16441 caacggcccc ggcactcgct gcaccctgtt tgtctccggg tgtgttcatg aatgccccgg
    16501 ttgctataac aaaagcacat ggcgggtaaa ttctggtcag ccatttacca aagcaatgga
    16561 agaccagatc attaacgatc tgaatgacac tcgtatcaaa cgccagggga tttccctctc
    16621 cggcggcgat ccgctgcatc cgcaaaacgt gccggatatt ctgaaactgg taaaacgcat
    16681 ccgcgccgag tgtccggata aagacatctg ggtgtggaca ggctataaac tcgacgatct
    16741 caacgctgcg caaatgcagg ttgttgattt gattaacgta ctggtcgacg gcaaatttgt
    16801 gcaggattta aaagaccctt ccctgatctg gcgcggcagc agcaatcagg tggtgcatca
    16861 tttgcgatga taagttccct gttacctgag tgcaggcaac agggaaatga ggaattaacg
    16921 atacttctgg tgataggcgt tgcgggtcgt tttcagttgc tctgcggcag cctgcgcttc
    16981 ttttacttta ccttcatttg ccagcttcag cgcgtcgtca atctgaccaa ccagaatatc
    17041 gaaaccgtga cggaagtctt tcatttccgg gctgtccggt gatttatctt cgagcttcgg
    17101 cggcgttgct ttttgcgcat ccagcgctgc ggcgcgcatc ttcgttaacg cgtctttgac
    17161 ttgcgccgcg ttatcggctt tttcgaccac ttttaaagtg tcgttgaggg tttccatatt
    17221 gtcttcaaga tcagcggcaa acgacgccga actgaatacc aacgaggaga ctgcaagaat
    17281 agctaacagg cttttacgca ttgctcactt ccttttttat ttacacttca tccttcacac
    17341 tgcctcatcg acggctacgc tcattcgccc gggtcacgta ctgcgagtac gctccccggc
    17401 cctcattcac tttccgtctc gatgcagtgc gaatgatttt gtgtaatttt ttagtttata
    17461 gcgcggcagg tcgcgccagt tttttactgc ccggcgattt tcatctccgg caacagcaca
    17521 gaaccacact gtatattact gcgtgtttca atatcgttac cgacggtgac aatattgcgc
    17581 cacatatctt ttaaattacc tgcgatggtg atttcgctaa ccggatactg aatttcgcca
    17641 ttctctaccc agaagcccgc tgcaccacgg gaataatcac cggtaatggc actcacgccc
    17701 tggcccatca gctccgtcac caccagcccg gtacccatct ctttgagcat ttgttcgaag
    17761 cttagccctt gtccggcaat ccgccagttg tgaataccgc ccgcatgtcc ggtgcttttc
    17821 agccccagtt tccgcgccga gtagctggtc agcagccact gagtcaggat gccatcttta
    17881 ataatatcgc gacgctcggt gcgcacacct tcgctgtcaa atggcgtcga tgccagccct
    17941 ttcagcagat gcggatgctc ttcaatggtc agccagtccg gcagaatttg tttacccagc
    18001 gaatccagca ggaaggtgga tttacggtaa accgatccac ccgctatcgc ccccaccaga
    18061 tggccaaaaa gcccggttgc cacttcattg gcaaaaatga ccggcgcttt catggtggag
    18121 agtttacgcg gtgacaggcg cgataaagta cggcgagcac agtcggcccc aacccactct
    18181 ggcgtttgca gatcgctcat cgcacgacca atggtgtagg cgtaatcacg ctccatatcg
    18241 ccattttctt cggcaattac acagctggag agcgaatgac gcgttgagca gtaaccctgc
    18301 aacatgccgt ggctgttgcc aaaaactttg acaccgtagt ggctgttaaa gctgccacct
    18361 tcggtattgg tgatgcgttt gtccgcctgc aatgccgcct gttctgcgcg ggccgccagt
    18421 tcaatggctt catccgggga aacttccgca gggtggaaca agtcgagatc tggtgcgtca
    18481 aaggccagca gctctttgtc tgccacgccg gcacagggat ctggcgaggt ataacgggca
    18541 atatccagcg ccgcctgtac agtacgggca atggcctgcg ggcttaaatc ggtggatgat
    18601 gcgctacctt tgcggttctg gtgataaaca gtgatcccca gcgcgccatc gctattgaat
    18661 tcaacattct ccacttcacc ataacgcgtg cttacgctaa tgccggtggt cttgctgacg
    18721 gcaacttccg caccgtccga tttgcctgag gccaactcca gcgcagtgga aactgcttct
    18781 tccagaatct tgcgctgcgc ttcaacttga gagattactt tcattgcaag tgccataatg
    18841 tagagagttt ctctgaagtc taacagagaa ccgtttttca gtgcgcatct taactggtaa
    18901 cattagcctc ttttttttaa ggagcctgag atgactaagc agcccgaaga ctggctcgac
    18961 gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt cagtaaaagt
    19021 gaaattaaac gtgatgccga ggagctaaaa cgccttggcg cggaaattgt tgatctgggg
    19081 aaaaacgcgc tggataagat cccgttagat gcggatctgc gcgcggctat tgagctcgcc
    19141 cagcgtatta agatggaagg tcgccgccgc cagttgcagc tcatcggtaa gatgctgcgc
    19201 cagcgcgacg tagagcctat tcgtcaggcg ctggataagc tgaaaaaccg tcacaaccag
    19261 caggtggtgc tgttccataa actggaaaac ctgcgcgatc gtctgatcga tcaaggtgat
    19321 gacgccatcg ctgaggtatt aaatctgtgg cccgatgccg atcgtcagca actgcgtact
    19381 ctgatccgca acgcgaagaa agagaaagaa gggaataagc cgccgaaatc cgcacgccag
    19441 attttccagt atctgcgcga actggcggag aatgaagggt aattttcgtt gttgatgtga
    19501 gtatggtttg ccggatgcgg tgcacctgta ggcctgataa gacgcatcaa gcgtcgcatc
    19561 aggcactatg ctcaaatgcc ggatgcggcg tgaacgcctt atccggccta cgcacactat
    19621 atcaggctaa ggccgaatta ctgcgcggct tccgccttct tcgccagacc atccagcagt
    19681 ttctgatgga tcccaccaaa accgccgttg ctcatcacca gaatatggtc gccaggctga
    19741 gcggttttca ccaccatatc tgccagcgta tccacatcgc cactccagtg tgcaggctga
    19801 acgcaggctt ctgccacttc tgccacctgc cacggaatat gcgccggttg cagcaggaag
    19861 acttcatcgg cacgacctaa tgaaggtgcc agatcgtctt tgcagatccc cattttcatg
    19921 gtattcgagc gcggttccag cacggcaata atgcgcgccg taccgccaac tttgccacgc
    19981 agcgccgcaa gcgttgccag aatcgccgtt gggtgatggg caaaatcgtc atataccgtg
    20041 acgccattcg cttcaccacg caactccaga cggcgacgag cgttaataaa cgaacccagc
    20101 gcgttagcgg catctgccgg cgctacacca acatggcgag ccgccgcaat cgccatcagg
    20161 ccattgtgca tattatgttc gcctaccagc gaccatttca cttcgcccac tttttcgcca
    20221 tccagcaaaa cttcccattc ggaagcatcg gtggtcagct ttttcgcctg ccagtgcccc
    20281 tgctcaccca ccagctcctg ctcgctccag cagcccatcg ccatggtctg tttcaggttg
    20341 atgtcgtttt ctggccagat aatacggccc tgccccggaa cgatacgcac cagatggtgg
    20401 aactgtttct ggatcgcttt caggtcgtca aagatatcgg cgtgatcgaa ctcaaggttg
    20461 ttgaggatca gcgtacgtgg gcagtaatgg acaaatttag agcgtttgtc gaagaaggcg
    20521 cagtcatact catccgcttc gataacaaag aagtcgcttt cgcccagacg cgccgaaacc
    20581 tcaaagttcc ccggcacacc gccgatcaca aatcccggtt tgtaaccgca ctgttccaga
    20641 atccaggttg ccattcccgc ggtggtggtt ttgccatgtg taccggcaac ggccagcacc
    20701 cagcggtcgc gcagcacaaa atcgtgcagc cactgtggac ctgacatata agggatgttt
    20761 ttttccagta ccgcttccac acacggattt ccacgggtca tggcgttgcc aataatcacc
    20821 agatccggct gcggatcgag ctggctggca tcgtaaccct gaatcagttc aatgccttgc
    20881 ttctcaagta aggtgctcat cggcggatac acattggcgt ccgaacccgt tacttcatgg
    20941 cctaactggc gcgccagcat cgccagaccg cccataaacg tgccacaaat tcctaaaata
    21001 tgaatgcgca tacgtcacta tcctttttta atctgccgct cattttacgc atatgtcggg
    21061 caagtgagaa acgcatttca ggataatccg caatttgctg gcgcgattca cctgagcgca
    21121 acattgagat tatttgttaa gattgttgcg gtcgctttac tccataaaca ttgcagggaa
    21181 agttttatga aaacgttagg tgaatttatt gtcgaaaagc agcacgagtt ttctcatgct
    21241 accggtgagc tcactgcttt gctgtcggca ataaaactgg gcgccaagat tatccatcgc
    21301 gatatcaaca aagcaggact ggttgatatc ctgggtgcca gcggtgctga gaacgtgcag
    21361 ggcgaggttc agcagaaact cgacttgttc gctaatgaaa aactgaaagc cgcactgaaa
    21421 gcacgcgata tcgttgcggg cattgcctct gaagaagaag atgagattgt cgtctttgaa
    21481 ggctgtgaac acgcaaaata cgtggtgctg atggacccac tggatggctc gtccaacatc
    21541 gatgttaacg tctctgtcgg taccattttc tccatctacc gccgcgttac gcctgttggc
    21601 acgccggtaa cggaagaaga tttcctccag cctggtaaca aacaggttgc ggcaggttac
    21661 gtggtatacg gctcctctac catgctggtt tacaccaccg gatgcggtgt tcacgccttt
    21721 acttacgatc cttcgctggg cgttttctgt ctgtgccagg aacggatgcg cttcccggag
    21781 aaaggcaaaa cctactccat caacgaagga aactacatta agtttccgaa cggggtgaag
    21841 aagtacatta aattctgcca ggaagaagat aagtccacca accgccctta tacctcacgt
    21901 tatatcggtt cactggtcgc ggatttccac cgtaacctgc tgaaaggcgg tatttatctc
    21961 tacccaagca ccgccagcca cccggacggc aaactgcgtt tgctgtatga gtgcaacccg
    22021 atggcattcc tggcggaaca agcgggcggt aaagcgagcg atggcaaaga gcgtattctg
    22081 gatatcatcc cggaaactct gcaccagcgc cgttcgttct ttgtcggcaa cgaccatatg
    22141 gttgaagatg tcgaacgctt tatccgtgag ttcccggacg cgtaatctcc tgcgcctctc
    22201 cacacggaga ggcgttttta tcgctgcgca atgttgactc ttgtcacagg cgaactctga
    22261 cgattctccc acagcaccgt taatccacgc tgtaatgcta taaaaataaa taacaaaata
    22321 ccgatggcga ttttcgtcca ccatgagctc agcgtgccat caaagttgat ataagtctgt
    22381 attagcccct gaatcgccac gccaaaaagc gtccctaata ccgttccaac gccaccgctc
    22441 agaagcgtac cgccaattac cactgaggcg atagcatcca gttccacacc tacgcccgcc
    22501 agcgcatatc cggcctgggt ataaatcgag aagacaatcc ccgccagcgt tgccagtccg
    22561 gtggagagca tataaatgcg aatagtggtg ctgcgagtgg aaatccccat caggttcgcc
    22621 gacgttgcgt tgccgccaat ggcgtatacc tgattaccaa aacgggtacg atgcgcgagg
    22681 aaaataccga taaccaccac cgccaacatc agcagtccca tcgcacttaa gcgaccgccg
    22741 ccagggattt tccacgcaag gcttgagagc gtgtcataaa tgggatggtt tatcggaatc
    22801 gactcttccg aaacgagata gctgacaccg cgcaaaaaga acatccccgc cagggtaatg
    22861 ataaatgccg ggatcttcag ggcgtcgatc aaaagcccca taaatgcgcc gaaggcacag
    22921 cccatcacca gcactagcgg aaacgccagt agcggcgaga ggccgaaatc gccaatcact
    22981 tttgccagaa acacgccagt aaaggcgatc accgaaccga cggagagatc gatcccaccg
    23041 gagaggatca caaaggtcat gccaacggca atgatcccaa gaaaggcgtt atcggtcagg
    23101 atattgcaga tcactcttgt ggaagcaaaa ccgggaaact gggtcaggca gtaaagataa
    23161 cccaacacaa agacgccgat ggtgatcatc agtggcaaat tacgttttat cacggctacg
    23221 tactccttta atcagactga taaagcgttg cgactggaca atcagcacgc aaagaaccac
    23281 caccgctttt acaacctggt tcatctctgg cggaaagccc gaaagcaaaa ttccggtgtt
    23341 catcccctga ataatcagcg cccccaccac cgaaagcagt aggttaaaac gtccgcccat
    23401 cagcgatccg ccgccaataa ccaccgcgag aatggcgtcc agctccagcc ataacccggc
    23461 gttattggca tcggcaccgc gaatatccgc cgccacgata atgcccgcaa tcgccgcaca
    23521 cagcccgctc aacacgtagg taagcatgac gatgatccgc gtgtttaccc cggcattttt
    23581 tgccgcccga atgttgatac caacggcttc gataaacatc cccagcgccg ttttgcgggt
    23641 caacagccag aacaggataa gcgtcagcac cgcgataatg accggcgttg gcaggaacaa
    23701 cagcgatcca ctgccaaacc atgagagatc cggcgagtta aacgtgacga tctgtccgga
    23761 ggtgatcagt tgcgccacgc cgcgcccggc gaccatcagg atcagggtgg caacaaacgg
    23821 ctgaattttg aggatcgcta ccagtatgcc gttccacaat cccgccagga tgccagtgcc
    23881 cagggcgctt aacaaaacaa tcggcaggct gaatcccgcg accgtcatcg cagccgttgt
    23941 ggctccggcg atagccatca ccgcccccac ggagagatca atcccacctg ttgcgatcac
    24001 cagcgtcatt ccgatagcca acagcgcaac gggggccgca cggttaagaa tgtctatggg
    24061 gctaccgaat aaacgcccat cctggagcac cacctgccag aaatgcgggg ccaccaggct
    24121 atcgaccagc agcaccagca acagtgccgc cagctgcggc attcccgttg gccagcgaaa
    24181 gcgccttttc ggcggcgtag tgtccgggag agattgaggc atcacactgt tctccttacg
    24241 ccgcaatggc gttcatgatc gccggaacgg aaagctctgc cagcgggatc tccgccacct
    24301 gtttgcgatc gcgcatgatg atcacccggt cggcatagcc caccagttct tccagttcgg
    24361 aggagatcac cagcagcgcc agaccatcgg cgcatagcgt ttcaatcagg cggatgatct
    24421 cggcgtgggc accaacatca atgccgcgag ttggctcatc aaggatcaga aattgcggtc
    24481 gggtcagtag ccaacgtgaa agcaacactt tttgctgatt gccgccagag agaaattcaa
    24541 tcggttgttc agttgaaggt gtgcgaatgc caagctggcg gataaagcgt tcggcaatct
    24601 cttgctgttc tttgcgggaa atgggacgta gccagccgcg ctgggcctgg agagcgagga
    24661 tgatattttc ccgcaccgag gcggcagcga tgatgccatc ggttttcctg tcttccgggc
    24721 agaagccaat gcccagtacc gaagcctgat gtggcgatcg caggttttgc ggtttgcctt
    24781 tgatcaacgc cgtgccgctg tcagcaggtt tgataccgaa gatcacttcg gcggtttcgg
    24841 tacgtcctga tcccagcaat ccagccagac cgacgatctc gccggggcgt acttcgagat
    24901 caaacggtgc gatcgttcct tttttgccgt aatttttgaa cgcggcaacg ggtttgtcgc
    24961 tcaacaatgt tcgcccggca cgctgtagcg cgtgggtatc cagctcgcgc cccagcatca
    25021 tttttaccag ttcgatctgc ggtagctcgc acgtttcccg acagcctacg aaactgccgt
    25081 tgcgtaagac ggtgatccga tcgctgaact gatagacctg atcgagaaag tgagtgacaa
    25141 aaatcaggct gacgccgcga tcgcgcaact gacgcatcag gtcaaacagt aactccactt
    25201 cctgggtgtc gagactggcg gtgggttcat cgaggatcag cactttggca gagagatcga
    25261 tagcccggca aatagcgacg atttgctgca tcgcgactga aaagcggttg agcggttcgc
    25321 gcacgtcgag ggagaaaccg taagatgcca tcagttcggt ggcgcgcttt tccatctctt
    25381 tgcggcgtag aaggccgaag cgtttgggtt cgcggcctat aaatagatta tcagcgaccg
    25441 acatattggg tagcaggttt acttcctgat agacggtgcc gataccgagt tgttgtgcat
    25501 gggcggtatt tttcggtgag atagcctggc cttccagcca gatggtgccg cgatcggcgt
    25561 ggtatacacc agttaatgct ttgattagcg ttgattttcc tgccccgttt tcaccgagca
    25621 gcgccatgat ttcgccgcgg cgcaggctga aatcaacgtt gtctaacgct ttgacgccgg
    25681 ggaaaaattt acttaatcct tcggtgcgga ggatctcctg gtgttggtta gtcgtcatgg
    25741 tttcccccgg tgtaaagcgt gctcccagta ggcatgataa gacgcggtaa gcgtcgcatc
    25801 aggcttcagc gcaccaccgc cggatgcggc gcgagcgcct tatccggcct acgaatcggc
    25861 atcaggcata gcaaccctca ataccccata tttttcttct tctctaactc ttcttttgcg
    25921 gtatcaggca gataaagggt ggatttggtc agcgtcagct tttcaggcat ggtgccgtct
    25981 tttttgtatt tctccagcgc gtcgaaggcg ggacctgcca tattcggcgt cagttcaaca
    26041 ctggcgttcg cttcgccatc aatcatcgct ttgtagatat ccggtacgcc gtcgatagaa
    26101 cctgtcagaa tatctttgcc tggtttcagg cccgcttctt taattgcctg aattgcacca
    26161 atcaccatgt cgtcgttatg ggcgtaaacc atgcagatgt ttttgccgtt gttttccgct
    26221 ttgataaagc tctccatgac ttctttgcct ttactgcggg tgaagtcacc tgactgcgag
    26281 cggataattt tgatatttgg cgcattctta atggcttcgg caaagccttt cttacggtca
    26341 atggcgacgc tggccccaac agtgccctgc agctccacca cgttgcatgg tttgccattc
    26401 acttctttta ccagccagtc accaatcaac ttgccttcga gaatgttgtc ggcagtgacg
    26461 gtggtcatat agagagattt gtctttcaca tcaatagaac gatcgagcaa gaagaccggg
    26521 atttcggcat ctttcgcctc ttttaatacc ggttcccaac cagtcgcaac caccggagcg
    26581 ataaagatcg catccacccc ttgcgcgacg aaggaacgta ccgctttaat ctggttttcc
    26641 tgcttttgct gaccatcggc aattttcagc gtaattccgc gtttttcggc ctcacttttc
    26701 gccacattgg tttctgcggc gcgccagcca gattccgatc cgacctgcga aaatcctacg
    26761 gttaatggag cggccaacgc catagacgac atggctgccg agactgcaga gactacaagt
    26821 aagcgtttcc acatagggtt gtcctcgtcg gggtaattat tattggtgaa aagatgttcg
    26881 cgaaaaaact atagacaatt cgttatgtaa cggattgcgt tacatcacac ttcaaagaag
    26941 taaataaaac gttaatcaca agtttgtaat cgctttcatc tcactatgaa aaatgcggct
    27001 acggttatgg attttcctgc tctgtatacc gtcttaaaat tggcgaaaaa ggaaaatgaa
    27061 gacgaaaaca agcgaagaca ttcggcgcga gttggctata atactcggca cttgtttgcc
    27121 acatattttt aaaggaaaca gacatgagct tactcaacgt ccctgcgggt aaagatctgc
    27181 cggaagacat ctacgttgtt attgagatcc cggctaacgc agatccgatc aaatacgaaa
    27241 tcgacaaaga gagcggcgca ctgttcgttg accgcttcat gtccaccgcg atgttctatc
    27301 cgtgcaacta cggttacatc aaccacaccc tgtctctgga cggtgacccg gttgacgtac
    27361 tggtcccgac tccgtacccg ctgcagccgg gttctgtgat ccgttgccgt ccggttggcg
    27421 ttctgaaaat gaccgacgaa gccggtgaag atgcgaaact gattgctgtt ccgcacacaa
    27481 agctgagcaa agaatacgat cacattaaag acgttaacga tctgccagag ctgctgaaag
    27541 cgcagatcgc tcacttcttc gagcactaca aagacctcga aaaaggcaag tgggtgaaag
    27601 ttgaaggttg ggaaaacgca gaagccgcta aagctgaaat cgttgcttcc ttcgagcgcg
    27661 caaagaataa ataagttctt ctagcgcaat aaccctgaac gccgggcttc ggttagtaag
    27721 ggttttttta tgcccgcgat aaataaactc tctattccac catcattatt ctcagcggtt
    27781 gcaaggcttg aacggtaaga acaagcaaac ccgaccacca ttttgctgtt catagccact
    27841 tgctggaagt tagccgacct cactcatact caccgttcaa agcgtgaccc cgttttaccc
    27901 ctgcacattt gtgaacgcca gcagagtttt ttatgcgatt gcagatatgg aaagcggctc
    27961 ggagagaaaa gcaaaagatg gaaaagttac aaaaaaaaat cgcgagatac atcgcataaa
    28021 acaacgccac ccgaaggtgg cgtgctttta ttattactta tccctgtcta accagtcgcc
    28081 gctttcaatt agctttaatc catcgacggg tcgttgataa acgtacatcc atgcactccc
    28141 gtacggcgtc tgaatcaact ggcgcgcgta ttcaccgccc ctggtgcgca aggcatcaag
    28201 ttcggccagc gtggcgttgt caatacgata aacttcaccg tgtactgttc cgttccccgg
    28261 aactgcgcct ggatagtggc ccaggctata caactggtag ttatcgatac tgaaatcgcc
    28321 cagtaactgg gcattggtca tccagtgact gttgccttgt ttgtggcgta aactgccgta
    28381 gacaaatatt cgcattgcta aaactcgaac tgatagagca aatccagtgc ctggtctaca
    28441 ccagacacgg cttccagata tagcttaggc atcaggcgat aacgtaacgt gagtgttgct
    28501 atagagtcaa atatacccac gccgtatttc acttgcagac ctggcaatac atagccgctg
    28561 accactacct gggaggagtc gcctactccc tgggtgtcga gcgctaaatt gcttacgcca
    28621 aacgtctcgc cgattttacc cacaatctgg ccactttgcg caacccccag accaatcagc
    28681 atcgaggtca ttgccgcact gtcgctctga tcgctctcca gcccttgtcc acgtagcaaa
    28741 taagacaatg cagcttgttg cgacatcgcc gggtcagaga agatctccgc tttcggttcg
    28801 tccgccagac cagtgacgcg aactccggcg attacgtcgt cttctgtagc atccgggtta
    28861 cgaatagctt caatattaag atacggttga tccggcggac cagagaacag taactcacct
    28921 ttacgcacaa tcagatcctg accataggca tggaagcgcc cttcagggat gttgatctgc
    28981 ccgttcaggc ccagcccttg tttgtcctga acgacgttaa gatcgcccgt cagccgcgct
    29041 ttcaggccaa aggcgtcaat gcgcacattg ttgccaacgt ggacaatcag gttgctgtta
    29101 atcggaatcg acgccgtttt tgcctcttcc ggttgcaggt tatcgttaag catcaccaca
    29161 tcgctggaga cgcccaccgc gctttccggc agatcgtgca ccacgatgcg cgcccacggg
    29221 acatccacgc gaccatcaag ggtaaacagg tttggtgtag cctcaaatac aacatctggt
    29281 gatacatcca ttcgtaccat cggcggcacg gtgatacgca ctttactgcc cttcgctgtt
    29341 acccgcgccc gccagttttc aatctggctc cagtcggcgt cgccgttcag gtagatctca
    29401 ccctgctggg tccgtactgt accggcaagc gtcgagcgca taccgttaaa gttgaccgca
    29461 agctggctcg gctgcatatc aaacggcata aagttgccgt cgatatccac acccgtaacc
    29521 tgtagctgcc caaacaactg cgggctttgc acatcaccac ccagacgtaa gttggcactc
    29581 accatccccg cagctttttc cccacgggta aagatggggt ttatcatcgc aaggttgaag
    29641 ttacggatat tgacgttgcc accaagatta cggcggcctt gcggatcggc cacctgcacc
    29701 tgtccatcaa actggccgtt attggtcagg cggatggtcc agcccaattc ggcacggttg
    29761 ttacgcaact ccgccgtcag attcagcgtc tgaaacgcca cgggcagcgc cgcatcgttg
    29821 acggtttgcg ttacctgcac gttacgcccc gaaagggtga tactgccctg cggcagcccc
    29881 tctttcgtgg tgtcccaggc gacatccgct ttacccgtga agataccgct ggcctgagtg
    29941 gtttctggca taaacggttt cagcatcgcc aggtcgaagc ggttgagatt caccaccgca
    30001 cgcccttcgg cccccgcatc gatagtttgc ggcacgcaca gttccgcatt cgggttaagc
    30061 cagcagtgtg gcccgatgct gattttttgc tccttgttgc gataatccag cgcaatatcg
    30121 cgggtcagcg accacgggcc gactggcgtc tggaagcggg tattgctaag cgttcccttc
    30181 cagcgttctt ctttgcgatc aaaacttcct gtcagattaa gctgtccgga aaccggttcg
    30241 ccctgaatcc gcaactgtag ctcgtgctgt ttttcgctac ctttggcatt cagggtgacg
    30301 aggttgatac tcacatcggg ctgcgagatt tgctcaacgc gtacgtcaag tttacccgca
    30361 atctgatcgg tggatttgat atcgccttcc acgcgaacct gcgccacgga aagttcctgc
    30421 cagcgcaggc cgcgcgcggt gatatctgcc agtagttgtg gcgcttccac cgtgccgcgt
    30481 actttcacca gccctttcgc tgtaccgcca agccccggca gcgcgttatc cagccccggc
    30541 gcgttgatgg tggcatcaag attgagatct tttaccccca gctcgccttt cacttcggca
    30601 ctgtttggcc ccagttccag atgcagccct gggatcttcc actgcatata actgttgcct
    30661 ttcagcgtgc cgtcaacgtt cactttgttc tgtttgacgt taccggtcag cttcagttct
    30721 ggcacctcca tctgccaggt gccgccgtac aggctaccgc gggttttaat caagccattg
    30781 agtttcgacg gccactccgg gaactctttg gcggtgttaa tgccgttaag cgttagctca
    30841 ccgcgccaac taatggcctg ctgccagtcg agcaacgctt tgagttcagt tttcccttcc
    30901 agcgccgcga cggtgagttt gtcgagattg acctgctgtt cattaccttt ggcatcaagg
    30961 gtaatggtgg ctggcgggat ctcctgtccc ttcactgccg tgcgcataga gagcgtgtaa
    31021 tcggtcattt tgccggtaag tttcagtttc agatcatccg cctgatactg cttctctcca
    31081 gtgaacggcc agtaaagctg tttgctgttc acttccacgt tgagcggcaa tccggcttcc
    31141 gccagtcgcg tctgggcgcg taaatccata tccaccggac cagaaaggtt aacgcctatc
    31201 tccagctgtt cgcgcagcgc gccgcccact ttcagcttca ccttttcacc tttcaacggc
    31261 tccacgttca gtgtgctgtt gagggtgata tccaccggcc agttgtctga cagctgcgcc
    31321 gtgccgctgg cgttgacaat cccctggttg gagtcgatat ccagcgcgtc cagtttggta
    31381 ttgccgtcga tgctgctcac tttcagcagc atggtgcgca cggtgatgtc tgtgtcgccc
    31441 gtcacgcgca gttgctcgcc cttaaactct tcaatgttca ggttgagcgg cagatgcacg
    31501 tcggtcattt ccggcaatac cgggcgagaa aagagatctt tcagcgtttc gccgagcggc
    31561 ttttcatccg gctgcggatt ttcaattttc ggttcgacaa cttcttcctg cgccacttcc
    31621 gccactttcg gcaaagcaat caacaggcct ttcagcgacg tcggtttcag ggtcagggtt
    31681 ttctcctgcc agttcaggcc ggaggtgaag tccatcaccg ataccgtggt gtcatcaatc
    31741 ttgatgttga cgttgtccag cgccacccgt gtcagggtga tgggatacgg cgtggagaga
    31801 tccagcgggc cgctatcttc ctcttcttca acctgttcag aaggaggcat ttttttactg
    31861 tcgatgttga cctgaatgtc tttcagcgcc aggtcattaa tacaaacgct actgttccac
    31921 aggcactcaa gcccgaccgc cagatgcaga ttgcccgctt ttaccgccac gcctggctgc
    31981 tcataacgaa cgtcagacaa ggtgagatcg cgccagccgc cggtgacttt gccaatatcc
    32041 agtcctggca cccagcgatc cgccgcttta aataccagat gcaggccgct ggtggtgccc
    32101 accagaaacg ccaccgatcc cagcaacagc aagataacga taaccacgcc gaggctgatt
    32161 tttttccata aactcataat tctggcccca gaccgatgta aaactgtaac ccgtgttcgt
    32221 ctttatccgc gaccggtacg gcaaaatcga gtttgattgg cccgaccggc gattcccagc
    32281 gcacgccgac cccggtaccg gttttaaagt cgctgcggcg aatatcgctt accgcttcgc
    32341 cgctatcgac aaacaccgcg ccccaccatt ttccggtcac gttgtactgg tactccagcg
    32401 atccggttat caacttcgag gcccctttca ggtcaccgtt ggcgtatttc ggagcgattg
    32461 atttgtattt gtagccgcga atactgcgat cgcccccggc gaagaaacgc agatccggcg
    32521 gtactttgtc gaaatcaccg gtttcaatcc agcccagcgt gccgcgtgta acaaaacgat
    32581 ggcgatcgta cagtgtgcgg atccagacgt tctgcgcctg gaaaacggag aaatcgacat
    32641 ctgagcccca ggccgtgttg gagtagtcga tagagtagcg ttgcgagtcg ccccaggttg
    32701 gcatcaggcc accgcgagaa cgcgtgcggc taatcatcac cccaggataa aacagcatcg
    32761 tggtgttggt aatttcaccc tgggtaaagt ggtcgagact ccagcgcagg ttaatggcac
    32821 gctgccagcc gctggagaga tcccagtagc gagaagccac cagcgtagtg gagtcagact
    32881 cggtatcgtt caggtcagtg cgcttaaaac cgccctgcac caaataatat tgttccagtg
    32941 gattcttcag cagcggcatt ttatagctga agtcgaggat ctgttccggc gcggaaatac
    33001 tggtactggt ggtcagactg tgaccgtatg agttcatcca cggttttttc cacgtcgctt
    33061 tcacgcgcgg tcccacgtcc gtagagtaac cgaccccggt ttcgatggtg ttttctgttc
    33121 gcggcgaaac cacgcccgtc aatggtaata ctttcgtttc gcgcgcttta tcaaattgtg
    33181 gagccaccac caccgagtta aaccagccgg tagcagaaag tcggcggttc agttccgcca
    33241 gatctttcga ttcgtactca tcgccctctt taaacggcac cagattttgc aggtattcat
    33301 cgcggatttg tgatccttca aaggtcacat gcccaaagcg gtaacgttcg ccactgttat
    33361 aatcaatatc ccagaaggct ttatgcaggc cgagcgcaat gcccagctgc gctttggtaa
    33421 attcgctatc gaaataacct ttacgcaacg caatgctggt taaggacttt ttgaaatttt
    33481 cataatcgcc ctggttcagc accgtgccaa tagccgggcg agtatcgagc aatttcaaat
    33541 agtctttatc ggtccgcgcg ccgccgcgca ataccacatc ggtgccgcca attaacaccg
    33601 gcacgcctgg cgtgactttg gcgatcaata cctgccgccc tttctttggc ggtggacgga
    33661 gatcaaattc aatggtcggc tggtaataac ccagcgcttt cagaccttcg cggatggcat
    33721 catcgacgcg tgcgcgaaag cgacggtctg gcgtcacttc atcactttca atcgtagaaa
    33781 gctgcgcacg aacgttcttt tccagctgtc ccgataaccc ctcgacctgt agacggacgt
    33841 tcgcggcgac ggcagatccg cttaagcaga gtaagcttac acagcataac tgtcggatat
    33901 agcgcacatt ttctcctgaa tatccttttt tcctgcccct ggaacgccgt taaacggctt
    33961 aacaaaaatc cagtaatatg gattaaaaag cagactaaac cccaaatatt tcttatgttt
    34021 tactttagac ctattcacgg tggttattgt gtgcaaatac gcctcttgtt acaaccttaa
    34081 ccccaatgac cgattttcgg gagagcgaca ccatgagttt atttgataaa aagcatctgg
    34141 tttcccccgc cgatgccctg cctggacgta acaccccgat gcccgtagcc acgctgcatg
    34201 cggtcaacgg tcactcaatg accaatgtac ctgacggaat ggagattgcc atttttgcga
    34261 tgggttgttt ctggggtgtg gagcgtctgt tctggcagtt acccggcgtt tacagcaccg
    34321 ccgcaggcta taccggcggc tatacgccaa atccgactta tcgggaagtg tgctccggtg
    34381 atacgggtca tgccgaagcg gtacgcattg tttacgatcc ttccgtcatc agctatgaac
    34441 agttgctaca agtattttgg gagaatcacg atcccgccca gggaatgcgt cagggcaatg
    34501 accacggcac gcagtatcgt tcagcgattt atccgctgac cccagaacag gatgccgcag
    34561 ctcgcgccag tctggaacgt tttcaggcgg cgatgcttgc cgccgatgac gatcgtcgca
    34621 tcaccacgga aatcgcgaac gccacaccgt tttattatgc cgaagatgac caccagcaat
    34681 atctgcataa aaacccgtat ggttactgtg gaattggcgg aattggcgtc tgtctgccgc
    34741 cggaagcata gcgttacggg tacaaatgta gattgttgat aaagtgctat ttgtctatgc
    34801 cggatgcggc gtaaacgcct tgccaggcct acaaaatcgt ccaaaatcaa tatattgcgt
    34861 ggactgcgta ggcctgatcg gcatagcgca tcagccagtt tttcatttat cagcagtctg
    34921 aattttaacc ctctggcggc tttacagtac cttacgctat actagccact gaaaatgccg
    34981 gttcactttc ttcgaatcgg ctttcaatgt gtatttcaca caaattaatc aacttcccct
    35041 tccgaggatc tggcctgaaa ggtcggataa gatatgttaa acagtatttt agtcatactc
    35101 tgtttgatcg ctgtaagtgc gttcttctcg atgtccgaga tctcgcttgc cgcttcacgc
    35161 aaaatcaaac ttaaactgct ggctgatgaa ggcaatataa atgcccaacg cgttctgaat
    35221 atgcaggaaa atcccggcat gttctttacc gtggtccaaa tcggtctgaa cgcagtggcg
    35281 attctcggcg gtatcgtcgg tgatgcggca ttttctccag cttttcacag cctgttctcc
    35341 cgctatatgt cggcagaact ctctgagcaa ctgagcttta ttctctcttt ctcgttagtg
    35401 actggcatgt ttatcctgtt tgcggattta accccgaaac gcatcggtat gattgcgcca
    35461 gaagcagtgg ctttgcgtat catcaacccg atgcgcttct gcctgtacgt ttgcaccccg
    35521 ctggtgtggt tcttcaacgg cctggcgaac atgatcttcc gtattttcaa actgccaatg
    35581 gtacgtaaag atgacatcac ttctgatgac atctacgcgg tagtggaagc cggtgcgctg
    35641 gcgggcgtgt tacgtaaaca ggaacacgag ctgattgaaa acgtctttga gctggaatcc
    35701 cgtaccgttc cgtcgtcaat gacaccgcgt gaaaacgtga tttggtttga tctccacgaa
    35761 gatgagcaaa gtctgaagaa taaggtggcg gaacatccgc actctaagtt cctcgtctgt
    35821 aatgaagata ttgaccacat catcggctat gtcgattcta aagacctgct gaaccgcgtg
    35881 ctggctaacc aaagcctggc actgaacagc ggcgtacaaa tccgcaacac gctgattgtg
    35941 ccggatacgt taaccctttc agaggcgttg gaaagtttta aaaccgcagg tgaagacttc
    36001 gcggtgatca tgaacgagta cgcgctggtg gtggggatca tcaccctcaa cgacgtgatg
    36061 accacgctga tgggcgatct ggtcggtcag gggctggaag agcagattgt cgcccgtgat
    36121 gagaactcat ggctgattga cggcggcact ccgattgacg acgtcatgcg cgtgctggat
    36181 attgacgagt tcccgcagtc gggcaactac gaaaccatcg gcggctttat gatgtttatg
    36241 ctgcgtaaga tcccgaaacg caccgattcg gtgaaattcg ccggctacaa atttgaagtg
    36301 gtggatatcg ataactaccg catcgaccag ctgctggtga cccgaatcga cagcaaggcc
    36361 accgcccttt cgccaaaact gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc
    36421 ccagaaacat caacggctcc tgaatcagga gccgtcttat tactgcatag cactttggtt
    36481 aagccatctc tgtttgcaga cgcataacct gacggttaac ttcggacatc actgacaaat
    36541 gcagcttatc cttcactttt gggataagaa tcttaccctt atcaaattca aaagcgccaa
    36601 cgtccttgat gtataaccgt ccacggaaca ggatctttac gtacttcgcc acctgaagtg
    36661 ggttgtaccg ttggaaaatt ttcattgtta tctcctgctg agtattacgc ctttgcggtg
    36721 ccacaatcgg cccaactatt atgaggcgca aattttaatg cctagtgact atagactatc
    36781 cgggcaatgt ttccaccgcg tataactttt tttacctaaa ggttacaatt attcagaatt
    36841 atctttttac cgaagcgcgg tcttcagtat aagcattcat ttttcatatg atttgtgcgc
    36901 ttgaccgcaa actggcatca cacttgcggg aaattcgata aatagcacat atgattaaaa
    36961 ctcagaccca agtggtcgga tcacctgcat atcataagaa ggaaacacca tgaccctacg
    37021 caagattctg gcactcacct gcctgctgtt gccgatgatg gcttccgcac atcagttcga
    37081 aaccggtcag cgagtgccgc cgattggcat taccgatcgt ggcgagttgg tgcttgataa
    37141 agatcagttt agctacaaaa cctggaacag cgcgcagtta gtgggaaaag tgcgagtact
    37201 gcaacatatt gctggtcgca cctctgcaaa agagaaaaac gcgacgctga ttgaagcgat
    37261 taaagcagcg aagttaccgc acgatcgtta ccagaccacc accattgtta acaccgacga
    37321 cgcgattccg ggttcaggga tgtttgtgcg cagcagtctg gagagtaata aaaagcttta
    37381 tccctggtcg cagtttattg tcgatagcaa tggcgtcgcg cgcggtgcct ggcagctgga
    37441 tgaagaaagt tccgctgtag tggtgctgga taaagacggt cgcgtgcaat gggccaaaga
    37501 cggggcgctc acaccggaag aggtacagca agtgatggac ctgctgcaaa agttgctcaa
    37561 ataatggcaa taacataaat tctgcaaaaa taattttccc ctatcgttat ctatgggata
    37621 ggggaattaa gcctctgagt tcttacgccg aactcatact ggtaacgcta gttgcttttt
    37681 tttaaacgcc tcaatggtcg atttatattt ctcgcaaatc ttttcttcgg taatatcact
    37741 atctgcggcc agattcgcac ttactatcgc atccacttgt tcggctaatt cgacttgttg
    37801 gcaacctgca ttaaccagaa aattataaat aagagagtaa tcttcatcct ccaaaacaac
    37861 agaggaacca ttacgaaaac gttcatcccc tccatagatt ttaagcgcaa agcccaaaga
    37921 ttctatgact atggcaaaat tatcatcaat tattttttcg taaatatacc atccatcgca
    37981 ttctttaatt ccacctcctg gaatttggag gtcaaattta ccccgtttta atttacttac
    38041 tatatcatcc aatacagcat aacctaaaga cacgtcacca aacgcctggc gaatataacc
    38101 tttaatcctt tccggtatgt cagactgttt tttctgcaga atatcccagg catacaatcg
    38161 tctgttttgt tctaacggaa actcgcggtt tttgaaatcc atgaatgagt tatactcatt
    38221 tattgttatg ggaatttctt cgtattcaaa tccactgcct cgcaatgtca ttatatattc
    38281 ggctccgtta tctttaaggc tgaaactgat aagaaactta tccatggcgt gaacattttc
    38341 attttttgat gaaagtacct gataactatt tttatcaatc tgagttgcgc aacgaaagaa
    38401 ctccctcaaa gttaacaggc tatcataatt tgaaatccaa tcttttttat taaatgcatg
    38461 ctggatattg ttaaaaaaat tgtttgaatg tgtttttgta tgcgatgatt ttacgaataa
    38521 ctccaccgca ccaataaaat tattgtgtct gatcctgggt aacatatggc taccttattt
//
LOCUS       DAYPHN010000027        38510 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000027 DAYPHN010000000
VERSION     DAYPHN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.027"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            108..223
                     /gene="rrf"
                     /locus_tag="ACG0BW_001368"
     rRNA            108..223
                     /gene="rrf"
                     /locus_tag="ACG0BW_001368"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(286..1044)
                     /gene="yhdZ"
                     /locus_tag="ACG0BW_001369"
     CDS             complement(286..1044)
                     /gene="yhdZ"
                     /locus_tag="ACG0BW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFK7258354.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLALHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1052..2155)
                     /gene="yhdY"
                     /locus_tag="ACG0BW_001370"
     CDS             complement(1052..2155)
                     /gene="yhdY"
                     /locus_tag="ACG0BW_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFK7258355.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPNRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2165..3346)
                     /gene="yhdX"
                     /locus_tag="ACG0BW_001371"
     CDS             complement(2165..3346)
                     /gene="yhdX"
                     /locus_tag="ACG0BW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFK7258356.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVIGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3414..4439)
                     /locus_tag="ACG0BW_001372"
     CDS             complement(3414..4439)
                     /locus_tag="ACG0BW_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7258357.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4870..5091)
                     /locus_tag="ACG0BW_001373"
     CDS             complement(4870..5091)
                     /locus_tag="ACG0BW_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258358.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5344..8448)
                     /gene="acrF"
                     /locus_tag="ACG0BW_001374"
     CDS             complement(5344..8448)
                     /gene="acrF"
                     /locus_tag="ACG0BW_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFK7258359.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8460..9617)
                     /gene="acrE"
                     /locus_tag="ACG0BW_001375"
     CDS             complement(8460..9617)
                     /gene="acrE"
                     /locus_tag="ACG0BW_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFK7258360.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADSASK"
     gene            10016..10678
                     /gene="acrS"
                     /locus_tag="ACG0BW_001376"
     CDS             10016..10678
                     /gene="acrS"
                     /locus_tag="ACG0BW_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFK7258361.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQRGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCIANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
     gene            complement(10681..10860)
                     /gene="yhdU"
                     /locus_tag="ACG0BW_001377"
     CDS             complement(10681..10860)
                     /gene="yhdU"
                     /locus_tag="ACG0BW_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFK7258362.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(10944..11828)
                     /gene="yhdJ"
                     /locus_tag="ACG0BW_001378"
     CDS             complement(10944..11828)
                     /gene="yhdJ"
                     /locus_tag="ACG0BW_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFK7258363.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILGSSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(11914..12210)
                     /gene="fis"
                     /locus_tag="ACG0BW_001379"
     CDS             complement(11914..12210)
                     /gene="fis"
                     /locus_tag="ACG0BW_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFK7258364.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12236..13201)
                     /gene="dusB"
                     /locus_tag="ACG0BW_001380"
     CDS             complement(12236..13201)
                     /gene="dusB"
                     /locus_tag="ACG0BW_001380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFK7258365.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13530..14411)
                     /gene="prmA"
                     /locus_tag="ACG0BW_001381"
     CDS             complement(13530..14411)
                     /gene="prmA"
                     /locus_tag="ACG0BW_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFK7258366.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14423..15874)
                     /gene="panF"
                     /locus_tag="ACG0BW_001382"
     CDS             complement(14423..15874)
                     /gene="panF"
                     /locus_tag="ACG0BW_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFK7258367.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(15864..16106)
                     /gene="yhdT"
                     /locus_tag="ACG0BW_001383"
     CDS             complement(15864..16106)
                     /gene="yhdT"
                     /locus_tag="ACG0BW_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFK7258368.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16215..17564)
                     /gene="accC"
                     /locus_tag="ACG0BW_001384"
     CDS             complement(16215..17564)
                     /gene="accC"
                     /locus_tag="ACG0BW_001384"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFK7258369.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17575..18045)
                     /gene="accB"
                     /locus_tag="ACG0BW_001385"
     CDS             complement(17575..18045)
                     /gene="accB"
                     /locus_tag="ACG0BW_001385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFK7258370.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(18450..18761)
                     /locus_tag="ACG0BW_001386"
                     /pseudo
     CDS             complement(18450..18761)
                     /locus_tag="ACG0BW_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            complement(18911..19741)
                     /locus_tag="ACG0BW_001387"
     CDS             complement(18911..19741)
                     /locus_tag="ACG0BW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258371.1"
                     /translation="MKSLFNRLTGKAVSRTAFVEHLGHEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLEEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRCEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(19778..20752)
                     /gene="yhdH"
                     /locus_tag="ACG0BW_001388"
     CDS             complement(19778..20752)
                     /gene="yhdH"
                     /locus_tag="ACG0BW_001388"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFK7258372.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILSRDEFAESRPLEKQLWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            20904..22844
                     /gene="csrD"
                     /locus_tag="ACG0BW_001389"
     CDS             20904..22844
                     /gene="csrD"
                     /locus_tag="ACG0BW_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFK7258373.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCERS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            23149..24192
                     /gene="mreB"
                     /locus_tag="ACG0BW_001390"
     CDS             23149..24192
                     /gene="mreB"
                     /locus_tag="ACG0BW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFK7258374.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            24258..25274
                     /gene="mreC"
                     /locus_tag="ACG0BW_001391"
     CDS             24258..25274
                     /gene="mreC"
                     /locus_tag="ACG0BW_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFK7258375.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPPQSGAQPPARA
                     PGGQ"
     gene            25274..25762
                     /gene="mreD"
                     /locus_tag="ACG0BW_001392"
     CDS             25274..25762
                     /gene="mreD"
                     /locus_tag="ACG0BW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFK7258376.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25771..26364
                     /gene="yhdE"
                     /locus_tag="ACG0BW_001393"
     CDS             25771..26364
                     /gene="yhdE"
                     /locus_tag="ACG0BW_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFK7258377.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAVQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            26354..27823
                     /gene="rng"
                     /locus_tag="ACG0BW_001394"
     CDS             26354..27823
                     /gene="rng"
                     /locus_tag="ACG0BW_001394"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFK7258378.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27891..31691
                     /gene="yhdP"
                     /locus_tag="ACG0BW_001395"
     CDS             27891..31691
                     /gene="yhdP"
                     /locus_tag="ACG0BW_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFK7258379.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWNQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DTDIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALNGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31847..33292
                     /gene="tldD"
                     /locus_tag="ACG0BW_001396"
     CDS             31847..33292
                     /gene="tldD"
                     /locus_tag="ACG0BW_001396"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFK7258380.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(33420..34349)
                     /gene="aaeR"
                     /locus_tag="ACG0BW_001397"
     CDS             complement(33420..34349)
                     /gene="aaeR"
                     /locus_tag="ACG0BW_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFK7258381.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            34532..34735
                     /gene="aaeX"
                     /locus_tag="ACG0BW_001398"
     CDS             34532..34735
                     /gene="aaeX"
                     /locus_tag="ACG0BW_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFK7258382.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34743..35675
                     /gene="aaeA"
                     /locus_tag="ACG0BW_001399"
     CDS             34743..35675
                     /gene="aaeA"
                     /locus_tag="ACG0BW_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFK7258383.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTSDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            35681..37648
                     /gene="aaeB"
                     /locus_tag="ACG0BW_001400"
     CDS             35681..37648
                     /gene="aaeB"
                     /locus_tag="ACG0BW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFK7258384.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            37740..38012
                     /gene="yhcO"
                     /locus_tag="ACG0BW_001401"
     CDS             37740..38012
                     /gene="yhcO"
                     /locus_tag="ACG0BW_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFK7258385.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(38068..38331)
                     /gene="yhcN"
                     /locus_tag="ACG0BW_001402"
     CDS             complement(38068..38331)
                     /gene="yhcN"
                     /locus_tag="ACG0BW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFK7258386.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         9538 a         9660 c         9940 g         9372 t
ORIGIN      
        1 ggctcagttg gtagagcgca cccttggtaa gggtgaggtc cccagttcga ctctgggtat
       61 cagcaccact ttttaggtta aagttcggca gattagaaaa gaatttgtct ggcggcagta
      121 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
      181 gtagtgtggg gtctccccat gcgagagtag ggaactgcca gacatcaaat caagcgaaag
      241 gccatccgaa aggatggcct ttttgctttt cgaactaaca ttcaattaat ggattacctg
      301 cgataaaaat gccctcgtac gctctgattt aggatgcgca aaaaattcat caggtgcagc
      361 ttgctccact atttccccac gatccataaa aatcaccctg tcagcgacgg ttcgtgcaaa
      421 ccccatctca tgtgttacac acaacattgt catacccgac tgcgccagcc caatcatcgt
      481 atccagcact tctttcacca tctccggatc gagcgctgac gtaggttcat caaatagcat
      541 gatctttggt ttcatacaaa gtgaacgtgc tatggcaacg cgttgctgct gaccaccaga
      601 aatctgtccg ggaaacttct gggcatgttc ggcaattctc acccgctcaa ggtaatgcaa
      661 cgccagatct tcagcctctt tcttaggcat cttgcgtacc caaatcggtg ccagggtaca
      721 gttctgtaga acggtcagat gagggaagag attgaaatgc tgaaagacca ttcccacttc
      781 ctgcctgacg cgctcaatat tgcggatatc ttcattaagt tcgatgccat ccaccacgat
      841 ccgtccctgt tgatgttctt ccagatgatt aatacaacga atggttgtcg atttaccgga
      901 accagaaggg ccacacagaa cgatccgttc tcccggttgc acggttaaat ttatattttt
      961 caaaacatgg aattgcccat accatttatt gacgttttcc agcgtaatca tcgcgttagc
     1021 aggttgcagt aaaatttggc tcatagtgtc ctcaatgcgg tgtacgcccg gtgttaaaac
     1081 gtttttccag atgttggcta taacgtgaca tgctgaaaca gaagatccag tagatcagtg
     1141 cggcgaaaac atacccttcc gtcgacatac ccaaccaggc gggatcaacg gttgcctgct
     1201 gaacgctact gaaaagatcg aacaacccga tgatgatcac caggctggta tctttgaaga
     1261 gtgcgatgat ggtatttacc agcccaggaa tcaccagctt caacgcctgt ggcagaataa
     1321 ccagcccctg ggttttccag taacccaacg ccagcgactc tgccgcttca tattgccctt
     1381 taggcagcgc ctgtaatccg cctcgcacga cttccgcaac atatgctgac tgaaacagga
     1441 tcacgccaac cagcgcgcgg atcaatttgt cgatactggt gccttctgcc ataaacaacg
     1501 gcagcatgac cgaagacata aacagaacgg taatcagcgg tacaccgcgc cagaactcga
     1561 taaaaatgac cgataagata cgcacaatcg gcatatggga gcggcgacct aacgccagta
     1621 agatccccca cggcagcgcc cccgcaatcc caactgatgc aataattaaa gttagcgtca
     1681 gcccgcccca ttgccgggtt tcaacccgct caagaccaag aaacccgcca tacatcagcc
     1741 accagacaat cagtgggtaa atcaccgccc ataccgcaat atagcgaccg cgattcggga
     1801 gtattttcca gaacattggt gcgatcgaca caagcccaat cagtaatgcg aggttaattc
     1861 gccagcgttg gtcgtgtggg taaagcccat acataaactg accaaatcgt tcgtggatga
     1921 agacccaaca ggcaccggct tttgtgcagt cggtacgcgt tgagccaacc cagttagcct
     1981 gcaaaaatgc ccagttcagc aacggtggaa tcagttccca catcaaccat atgcagccaa
     2041 tagtcagcag gctattgctc cagctggaga acagattttt tcgcacccac accatcgcgc
     2101 ggcttgagtt atggctcgcc gggcgcgggg gatgagacag caatactttt gtcatcatga
     2161 ctccttagcg ttcaacgatc gcgatgcggc ggttatagat attcatcagc agcgagatag
     2221 tcaggctgat aatcagatag accgacatgg tcatggctat cgtctcgatg gcttgccccg
     2281 tctggttcag cacggtgcca gcaaacagcg aaaccatatc cggatagcca atcgcggcgg
     2341 caagagagga gtttttgacg atgttgagat actggctggt taacggcgga ataatcactc
     2401 gcagtgcctg gggaataatg acctggcgta gcgtaaccgg attgggtaat cccagtgacc
     2461 gagccgcttc atgttgacca taaggcactg cctggatccc agcgcggata atctcggcga
     2521 taaatgcaga ggtataaacc gaaagcgcca gcgttaaggc tgccagttca ggaattaaaa
     2581 ccatcccgcc acggaagtta aagcctcgta gggctgggac atcccagtgt agtgctgcgc
     2641 caaaaagcca ttgcgccagt aaaggcaaac caatgatcaa cacagcggcg atcggccagg
     2701 tgcggcgcag ttgtccggtc tttatctggt atgttttatt aaagcggaat agcccaacag
     2761 aaaggactat agccataaca acagccagga taaacgcaat aaatccgtct cccagctgcg
     2821 gtgacggaat ataaagccca cgattgctca aaaaggccag atcaaacgcg ctcacagcct
     2881 ggcgtggtcc gggcaaattg cgcaacacgg caaagtacca gaagaagatt tgcagcagcg
     2941 gcggaatatt acggaagatc tcaatataaa ttgtggaaag ctttcgtagc agccagttat
     3001 ccgaaagtcg agccagaccg ataaagaagc ccagaacaga agcgaataca atacacaatg
     3061 cagaaaccag tagcgtattg agtaagccaa caatgaaaac gcgtccgtag gtatcaccct
     3121 gctggtaatc gatcaaatgc tggacaatac cgaagccagc gccgcgatcc agaaaggcaa
     3181 aacctgaagt aatgccacga ttactgagat tcgttacagt gttgtgaaat aaccaaccaa
     3241 caatgccgat aacagcaaca acggcaagga tctggaataa ccaggcacga accgtagggt
     3301 tggcaaagga gagtgagcct ttaacggttg agcggcgatg agacatagaa acctcggaaa
     3361 gcataacgct gaagagagcg ccgccgtagc gacgctcgtt acctcacacc gacttaacgc
     3421 acgggcggcg cgtactgaat accgccgtta ttccagagat tattttgccc acgtttaatt
     3481 ttcagcgggc tttctgaacc tacgttacgc tcaaaaattt ccgagtagtt acccacctgt
     3541 ttgatgatgt tataggccca tttattatcc aacttcagat ccttgccgta atcgccttct
     3601 ttacccagca aatgtgccat atcaggcgtt gctggattag ccgctttttc atcgacgttc
     3661 tgggaattga tgcccatctc ttcagcattc agcatggcga aaagcgtcca gcgtacaatc
     3721 gagaaccatt catcatcgcc acgacgaact accggcccaa gcggttcttt ggagataact
     3781 tccggtaaga caatccattc agccgggttg cttaatttga tgcgcagggc atacagttgt
     3841 gattgatccg aggccagcgt atcacagcga ccagattcca gtgccttcgc tgattcgtca
     3901 gagcgatcga aagtcactgg tgtgtacttc atattgtttg ccttgaagta atcggcaaca
     3961 ttgagttcgg tatcagtacc cgcctgaata cagacagtag cgccatccag ttctttcgcg
     4021 ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc cgtcgtaata agtgacgcca
     4081 gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc aggtcgtatt acgggagagc
     4141 aaatccacct cccctgactg taaagccgtg aagcgttctt ttgcagtgag cggggtatat
     4201 ttcactttcg tgtcgtcacc aaatacagct gccgcaacac cacgacaaac atctacatca
     4261 atacctgaaa acttaccgtc agcatcggca taagagaacc caggtaatcc atcactgatc
     4321 ccgcattgca caaaaccttt tttctgaacg gcatcaagcg tcgcgccagc atgcgcctga
     4381 tttgcaacgg caagcagcac gctggcggca gccagtgtgg ctatcatcat ctttttcata
     4441 atgcttcctg tgaggcgaaa attatcgtta taggacgttt gggaaacgtc ttttacctgt
     4501 ctgtggccat cctcttataa gcaaaagtag tgccagcttt tgcgcacgat ttcgcgtcgc
     4561 aataagctga gtgtagacag gaaataataa aaacagcgcc ccgcaatggt gcgaaaatga
     4621 aacatgcgcc acattttggc gcaaaatatt tacgcgtttt ttactaagca gaataatgat
     4681 tgttagcgtg agtataattc tggtgggaag tgtgaaaatt aaattgatgc gttatttcga
     4741 aagcagcgaa ataacgcgat ataacatgct aaatcagcag ggaattagcc agcagaatat
     4801 agcaatattt tatcttatgg tgagatactg tatcgttaaa cagagtgcgg tactccgcgc
     4861 cctgtgcagt tatttcgaca gagcgatgat ccccatcgtc aggccagcaa cagctgctgc
     4921 cccgccagca atagccccag ccgtttccca gttatcctga gtcgtgcctt tcatacaggt
     4981 attagtgtga accagtcgac cctgatcgtc ataaaccgga acgcagggcg aatcgtgagc
     5041 acagcccgcc agcaatgcgg tgagcaatgc cacaggaatt aatcttttca tggtgttacc
     5101 tcagtaataa acgctaacgc ttaataagtt aactctctta ttaacgagct gaatgccatt
     5161 ttatcaccgg aggttataaa tcttgcggaa tgaataatga caaattatgt gaattgtaaa
     5221 aatagtgaag ggagcgggaa ctatagaaag tttttaaaga gtgagaagaa gaatatctta
     5281 ttaattttta tgggtttcac tgaaaataat aaaggcaccc gaaagtgcct ttatgtttct
     5341 gatttatcct ttaaagcaac ggcggatcac cacaaagaac accggcacga agaagattgc
     5401 cagcaacgtt gcagaaacca tcccgcccat tactccgata cccactgcgt tctgcgcgcc
     5461 actgccggca ccgttactga tagctagcgg taatacgccg agaataaagg cgagagaggt
     5521 catcaggata ggacgcagac gcatacgtac tgccattagt gtcgcttcaa caacaccttt
     5581 accctctttc tccatgagat ctttagcgaa ctcaacaatc aaaatagcgt ttttagccga
     5641 caagccaatt gtcgttagca agcccaccat aaagtagacg tcattttttt gattaaagag
     5701 tgtcgccgcc agtagcacgc cgacaatccc taacggcacg accaacataa ccgagacagg
     5761 aattgaccag ctttcataga gtgcagcaag gcacaggaaa acaaccacaa aggaaattgc
     5821 taccagagcg ggagcctggt ttcccgataa gcgttcctga tatgacatac ccgtccagtc
     5881 ataaccaatg ccagctggta attgtgatgc aaggttttcc atcaacgcca tggcatcgcc
     5941 ggaactggtt cctggcgcgg cttccccctg aatctccatt gacggcagac cgttgtagcg
     6001 ctccagtcgc ggagagccat acacccaatg tgcagtggta aaggccgaga atggcaccat
     6061 ttcgccgttg gcgctgcgga cataaagttt atcgacatct tctggcagca tacggaattt
     6121 ggcatccgcc tgaacataca cctttttcac gcgaccacgg tcaatgaagt cgttaacgta
     6181 agtcccacct aacgccgttg aaatggtctg attgatgtca gaaagtgaaa cacctaatgc
     6241 ctgcgccttt tcctggtcaa cttccagttt aaactgcgcg gtgtcttcca gaccattagg
     6301 acgcacgctg actaagctgg caggatgttg tgccgccatg ccaagcaact ggttacgggc
     6361 ctgggtcagg gcatcatgac ccagcccagc ctgatcaatt aactcaaagt cgaaacccgt
     6421 tgccgtgccc agttcaacaa tggctggcat attgaatgga atgacaaaac cgtcgcggat
     6481 cttgcccaat tccattttgg cacgatggat taccgcttcc gcactgtttt cgtcaccact
     6541 acgctcttcc cacggtttca gactgacgaa agccataccg gcgttttgtg cctggccgct
     6601 gaagctaaag ccgttaaccg taaagacact ttctacgttc gctttctcgt tcttcagata
     6661 gtaatccgtc acttgatcca acactttttg cgttcgctct tgcgtcgcgc cagcgggtaa
     6721 ctgaatcata gtcagaaaga caccctgatc ctcttcaggt aagaaggaag acggaagacg
     6781 taaaaacaac accaccattc cagcaacaat tagtgcatag atcagtaaat atcgtcctgt
     6841 ggaaccgagg attttgccga cgctgttggt gtagtggtta acgctatgat cgaaggtggt
     6901 attaaaccag ccgaagaaac cgcccttatt ttcgtgatgc tcagcagaga cgggtttgag
     6961 cagtgttgca cataacgctg gggtaagaat caatgccacc agaacagaaa gcgccattgc
     7021 cgaaacgatg gtgatagaga actggcgata aattgcccca gtagaaccgc cgaagaatgc
     7081 catcggaata aataccgctg acagcaccat tgcgataccc accagtgcgc cctggatttg
     7141 cgacattgat ttttccgtcg cttcttttgg cgggagctta tcctccatca tcacgcgctc
     7201 gacgttctcc accaccacta tcgcatcatc gacgagcagc ccgatggcaa gcaccatccc
     7261 gaacatcgtt agtgtgttga tggagtaacc aaaagcggcg aggatggcaa acgtccctaa
     7321 caacaccacg ggtaccgcaa tggtggggat cagcgttgct cgcatattct gcaagaacag
     7381 atacatcacc aggaacacca gcataatagc ttcgaacagc gtttttacca cttcgtgaat
     7441 agaaagctgg acgaatggcg tggtgtcata agggtagaga accttcattc cctgcgggaa
     7501 gaatggctgt aattccgcca gttttgcctt aatggctttc gcggtatcga gagcattcgc
     7561 gccggttgcc agcttaatcc ccaggcccgc cgccggttta ccgttgatgc gagcgataac
     7621 gttatagttt tcaccgccaa gttcaacccg tgcgacatct ttcaggcgta ccaccgagcc
     7681 gtcactgttt acgcgcaagg tcactttgcc gaattcttcc ggatttttta accgcgtctg
     7741 agcaataatc gaggcgttca actgttgccc tggtaacgct ggcgttccgc ccaactgtcc
     7801 ggcagcgatc tgatcgttct gtaccttcaa ctggttaatc acatcaacag gcgtcagttt
     7861 atatttgttt agcagatcgg cgtccaacca gatacgcatc gcatactgtg cgccgaaaag
     7921 ctgtacgtca ccgacgccat tcagacggct aagcgtatct ttaacgttag aggccacata
     7981 gtccgagata tcgtcctgtg tggtgtctgg gttatcagag acaaagcccg ccaccatcaa
     8041 atagctgcta ctggactttt caacactgat cccctgctgc tgtacttcct gcggcagcaa
     8101 cggcgtggcg agctggagtt tgttctgcac ctgcacttgc gcgatatcag gatcggtccc
     8161 ggactggaag gtaagggtaa ttgtcacgct accggcggaa tcgctggtgg aggacatata
     8221 catcaggtta tcgataccgt tcatattctg ttcgataacc tgcgtcaccg tatcctgcac
     8281 ggtctgcgca tcagcgcccg gatagtttgc tgaaacagaa accgcaggcg gtgcaatggt
     8341 tgggtattgg gcgacgggca attgtaggat cgccagtgcg cccgccatca tcagaataat
     8401 ggccagcacc catgcaaata tcggtcgtcg aataaaaaag tttgccatgt cagattgcct
     8461 tacttcgatg cagaatctgc gggggtatcg gtagtggctt taacctgctc tcccggacgc
     8521 gctttttgca ggccgctgac aatgacttga tcgccagatt tcagaccttc gctaatcaac
     8581 catttatcgc caatcgcctg actggcaacg acagggcgcg cttcaacctg gcttttatcg
     8641 ttaacaatca gcacggttgc atcaccacgc ggtgtgcggc taacgccttg ttgcgggata
     8701 agaatggcgt caggttggac gccttcatca atccgtgcac gcacaaacat acccggcaaa
     8761 agcgtatgtt gcgggttagg gaagacagca cgtagggtta tggagccggt gctttcatca
     8821 acggtcacat cagagaattg cagcgtacct ttcaggggat aggtttgacc gttttccatg
     8881 accaactcta cgttgctggt ggcgttttcc ttatgcaaat ttccttgctc tacggattgc
     8941 ttcagcctca taaaatcgtt gctggattgg gtcacatcaa cgtagatagg atcgagctgc
     9001 tggacagtcg ccagttcagt cgtttgccca ttagtgacaa gagcgccttc ggtcacggtc
     9061 gatttgccga tacgtccgct aattggcgca gtcactttgg tataagcgag attgatgcga
     9121 gcgctttcga ccgtggcttt tgcggcaatc acggcggcat cggcctggcg agcatcagca
     9181 atggcctggt cgtactcctg ctgactgatg tatttcgtac ccacgagcgg aacgtaacgt
     9241 tttaccgtca aatgcgcgat ggcggcggcg gcttcacttt tcgccagttc gcctttcgcg
     9301 ctgtcataac ttgcctgata ggtcgcggga tcgatctggt acagggactg gcctgcttgc
     9361 acatcgctgc cttcagtgaa attgcgattc agtacgatcc cgctaacctg tgggcgaact
     9421 tcggctatac ggtaagcatt ggtgcggcct ggtaattcag tcttaacttc taacggggcc
     9481 gttttcacaa tatgaacggt aacctgcggt tcaccgacgt gagctttctc ttctccctta
     9541 tcgttacaac cggcgattaa agccgcggag atcagaataa aggagggcag gaggaaaaac
     9601 ctggcatgtt tcgtcattac tattcctcaa aaaaccaaaa gcgcgttatt tacccaaaaa
     9661 ggcaacgcgt taactcgcag aaagaaaaat acagttcgct atcctacaaa ttatcattcg
     9721 tcgatgtaag gaatagttat gaatactggc gtctcaaggc acataaacac aaaaaaagat
     9781 taatattcta ctgttttatt ttgacgcggg ttgaaagagg tagaatcaaa tcctcgtaaa
     9841 ttgaaatata tattgataga gtgaatgtat tttaggtaaa taataaacgt tatttatttg
     9901 acgatttata gcaactcaat attagcctct cgtataaata tacattaggt gatagattaa
     9961 ccttcgctat tttctcatcc tgtgtcgaat atatttattt cctgaataat taatcatggc
    10021 aaaaagaacc aaagctgaag ccctgaagac ccggcaagaa ctgattgaaa ctgccatcgc
    10081 ccagtttgcg cagcgtggcg taagcaagac gacgctcaac gacattgccg acgccgctaa
    10141 cgttacgcgt ggcgctatct actggcactt cgagaacaag acccaactgt ttaatgagat
    10201 gtggttgcaa cagccttcat tgcgggagtt aatccaggat cacttgactg ctgggttaga
    10261 gcatgacccg tttcagcaat tgcgtgaaaa attgattgtt ggcttgcaat atattgccaa
    10321 aattccccgc cagcaggcgt tgctgaaaat cttatatcac aaatgtgaat ttaatgatga
    10381 gatgctggca gagggagtga tacgcgaaaa gatgggtttt aatccgcaga ctctccgcga
    10441 agtattgcag gcatgtcagc aacaaggttg tatagcaaat aatctcgatt tagatgttgt
    10501 gatgattatt attgatggtg ccttcagcgg aattgttcaa aactggttaa tgaatatggc
    10561 gggttatgat ctttataaac aagcacccgc actggtcgat aacgtattaa gaatgttcat
    10621 gccagatgaa aacataacga aattaattca taaaacgaat gaattaagtg tcatataaat
    10681 tcacattctg tcctgaaaat tcagttcgtc acagttaacc agttttagtg gattaactga
    10741 gttcatattg cggcatttgt ctgtttctgt tgcgagcagt tcaatccact gcatcagtaa
    10801 agtatcaaac agaaaaacga agacagcgaa aacgacgagc caccaatact tgcgaatcat
    10861 tacctgagcc tgtgcatgtt taaccaagtc tggcaggaaa tatacacgaa atgaataatc
    10921 tgaaaaccag gcttacactt cctttacttt gcaatgagat cggggtcaac ttcgcttaac
    10981 ctgcttcgtt ttgacaggtt gccggtcttt ctttttttca ctttcgccag ttcttccgca
    11041 gagtaatgcg acgcgacatc cagccgtcga agccccattt tgatgtactc gctgttgatc
    11101 tcaataccaa tgaattttcg cccactggcg acggctacgg caccggtagt aaagctacca
    11161 gcaaacgggt cgagaacgat atcgcctggg ttggaagagc cgagaataat gcgtttcagt
    11221 aaggcttccg gtttttgcgt cgggtggttt tcatattcat ccatcaaata acgcacgcgc
    11281 ggaaaatccc agacgttacc cggtactttt tgatgattgt aaggctgtgg agggtttttg
    11341 cgataatcga tcaacgcgcg ctgcgatccg gttttggctt ctaccagaat agcatcaccg
    11401 ttgaatgtgt agttctttgc gtctttcacc atcatcagga tgggttcgta catggagccg
    11461 tagtgttttt tcgcctgcac tccagaactg tcatatgacc agacgatgcg acttttgatg
    11521 gtaaaaagct tgcggcactg gagatcgata aagggcatgt tttccgtgct attcatgatg
    11581 tacatgctgc cctgcttttt cagaacgcga tggcactctg caatcacttc aaacagccag
    11641 tcgataaaca gatcttcttt ccaggcttcg atcagaccat caaaattttt accgatgtta
    11701 tacggtgggt cggcaaagat cagatcgaca ctttcggcgg ggagcttttt aagttcggtt
    11761 aatgcatcac cctggatgat ggtctttgct tcattaccaa atcgggtcgg ttcacatcct
    11821 gttctcatgg tcactccctt tgtgacacct ataaaaaagg cgcttcccca tgccgagtag
    11881 cgccttttta atcaagcatt tagctaacct gaattagttc atgccgtatt ttttcaattt
    11941 tttacgcagc gtaccacggt tgatgcccat catcagcgca gcacgggtct ggttaccacg
    12001 ggtgtattgc atcaccatgt ccaacagggg ctgttctact tcagccagta ccagctcata
    12061 gaggtcattc acatcctgac cgttcagttg agcaaaatag ttcttcagtg cctgtttaac
    12121 cgagtcacgc aggggttttt gggttacctg atcctgagag ttaacggtag aaacggtcag
    12181 tacgtcagaa tttacgcgtt gttcgaacat agttctgtca gctctttatt tctgtttacg
    12241 caaaattttc gaagtatgcc tccaacgcct ccagctgttc gctggcatcc tcaatggcgt
    12301 tgaatgtgcg ccgaaactgg tcatttggag cgtgttcctg gagataccag gaaacgtgtt
    12361 tacgtgcaat tcggtaccct tttgccggac cataaaagtc atgcagttcc cgaacgtgcg
    12421 cgcaaagcaa gcgcttaacc tctgccaaag gcagcggggg cagcaactcc ccagtgtcca
    12481 gataatgctg gatttcccga aagatccagg gtcttccctg agctgcgcgg cctatcatca
    12541 aggcatccgc ccctgtatag tcgagcacag ctctggcttt aagcgggtca gtaatgtcgc
    12601 cattcgcgat aaccggaatg gaaactttct gcttaactgc ccgaatactg tcgtactcag
    12661 cttctccatt gaacaaacag gcgcgtgtac ggccatgaat ggtcagagcc tgaatgccac
    12721 agtcttcagc cagttgggca atctcttcgc agttacggtg ttccggtgcc cagccagtgc
    12781 gaattttcag ggtaacagga acgtccactg cattgacgac ctcggtaagg atcgatttaa
    12841 cgacatccgg gtactgcaag agggctgaac ctgcgagctt gcgattcact tttttagccg
    12901 ggcaacccat attgatatca ataatctggg caccgctttc cacgttaata cgtgctgcat
    12961 ctgccatttc tttcggatcg ctaccagcaa tttgcacggt gcgaataccg ggttcatcaa
    13021 tgtgcaccat ccgtaaacga gatttgtcgc tttcccaaac ctgagggtta gaagacatca
    13081 tctcggatac tgtcaatccg gctcccatct cgtagcacaa cgtccgaaaa ggtctgtctg
    13141 taatgccagc catgggcgct gcgatcaggc gatttctgag ctgatattgt ccgatgcgca
    13201 tgagttaaga aatgaccata ctgtgactgc aaggcggcgt atattacgca ttttttgcac
    13261 gagatgaaag gccaaacttt gaccaatcct ctgagatgga tcaaagaatt gcatttaaaa
    13321 tgagcgtagc gcgataatta ctcataaaaa tcatcatatt agaaaatagt gactaaaatt
    13381 tacactcaaa gaaatttgag taagttctca atttttcttt atgaatgaaa attttggcac
    13441 gcaaatttgc gtaaataatc ggcaaattaa cgtgcctttt gtgacatcgc tcgcacttcg
    13501 ctctgcgtca ccctacggcg atgcgaaggt taattcttac gaccggtaat gcggcaccac
    13561 tcttcttttt ccacgaccgg gtccagagcg aagctgtcgg cataagcttc gcaaacgctc
    13621 tctgcctggc ttgccagaat accggaaagg cccagcaaac cgcctgaaac tggcaggacg
    13681 ctgattaacg gtgccagttc acgtaatggg cctgcaagga tgttagcgac caccacgtcg
    13741 gctttcattt cttctggctg atctttcggt aagtagagtt ccagacggtc agagacgcca
    13801 ttacgttccg cgttgtcgcg gctggcctga atcgcctgcg gatcaatatc aataccaatg
    13861 gcttttgctg cacccagttt cagcgccgcg atagccagaa tgccggaacc gcagccaaag
    13921 tcgatgactg ttttaccggt taaatcgagg ctgtcgagcc attgcaggca cagagaggta
    13981 gttggatggg tacccgtacc aaacgccagc cctggatcta acatcacgtt gacggcgttt
    14041 tcgtccggca catcacgcca gcttgggcag atccacagtc gttcaccaaa gcgcatcggg
    14101 tggaaattat ccatccattc gcgctcccag tctttatctt ccagttgttc gattttatgc
    14161 gcgaagcccg cgccgagcag cggatggttt tccagaatcg ccaccacgtc gttcatatcg
    14221 gtttcagcgt cgaacagacc aatcacatcg gtatcgcccc acaggcgcgt ttcgcccggc
    14281 agcggctcaa atacaggcgt atcgtgggta tcctgaaaag tgatagaaac ggcacccgct
    14341 tccatcagcg catcgctaag atcttccgcg ttcgcgccgg tagtgttcag tttcagttgg
    14401 atccaaggca tggcaaaact ctttatttat cagtagtcaa aacggtagct tgcgggacgg
    14461 atgtaccgaa acggtttccg accaggaaag ccagcaaact tagtagtaac gagggcacga
    14521 tagggtggaa gcccaggtac tgaatattca gcgtcgcgag tacggcatac agtacgccgc
    14581 caacaatcat cgcacttagc gcgcctttgg cgttggcgcg ttcccagtaa agacccagta
    14641 ccagcggcca caggaaaacg gcttccagcc caccgaaggc cagcaaattc agccagatga
    14701 tcatttctgg cggcttccag gcggcaagca gcagcaacgc accgagaact aacgtaatta
    14761 ccgccgacat ccgcttcaga cgcgtctcgt tttgcatttg atccggacgg atattcaaat
    14821 agagatcttt aatgatcgta gcggaacttt gcagcaactg ggcattgatc gtcgacatga
    14881 tcgcagccat aggtgcagcc aggaagatcc cggcagcaaa cggtggcaac acttttacca
    14941 ttaacgtcgg gatcaccagg tccggtacgg tgagatcggg gatcaccgcc cgacctaacg
    15001 ctccggccag gtgcataccg aacatcagga ttgcgaccac aatcgtaccg atgatgatcc
    15061 cccgatgtac ggctttgctg tctttataag agatacagcg caccgcagta tgcggcaggc
    15121 caatcacgcc aaaacacacc agcacccaga acgacgtcat aaaggcaggc gacagaatat
    15181 cgtcagcgcc ttgtggcgta accagttgcg gatcgatggt ttgcaacgtc tctactgcgt
    15241 tgcttaagcc acctgcggca tgtaccacgc caataagcag cacaatggtg ccaatcagca
    15301 tcaccagccc ttgcatggtg tcgttcagca cgctggcgcg aaagccgccg aaggcggtgt
    15361 agagcgcaat gctgatgcca aaaatcagca aacctgtttc ataagggata cccgccgcgg
    15421 tttccagcag acgcgcaccg ccgataaact gcacggtcat cgcaccaacg aacgcaacca
    15481 gcaaacttaa actcgccagc cacaccagca gacgactctg gtagcgggca aatagcatat
    15541 cgttcagcgt caccgcattg tagcggcgcg caagaatcgc gaacttcttg ccgagaatac
    15601 cgagcgaaag ccagaccgca ggaagctgaa tcatcgccag caatacccaa cccaggccgt
    15661 atttataagc agcgccaggc ccgccgataa acgaactggc actgatatag gtcgcggtga
    15721 gcgtcatcgc cagcacaata ccgcccatag agcggctgcc gaggaaatac tcattaagga
    15781 aggtgccggt gctccgttta cgcatcgcat aaacagagat accgaacacc accaccagat
    15841 aggcgaccag cggtagaatc acttcaagct gcatcgtcat cctccagtgg gatatcgcga
    15901 tagataaatt tcaccatcgc ccagcacagt ccaataaaca gcagcggcgt caggatgcag
    15961 gccatctcaa accagcgcgg aaagccggta aaaccggggg caacgccagg taagtaagcg
    16021 gctactaacc ataccgccag atacaaaagg gtcagcccca gcgcccagcg cgcctcttta
    16081 tgggcctgaa caaaacgagt gtccattttt tgtccctgat gggtgaagaa agcggggatt
    16141 gtaccttatg ggggttgtcg atccccagta ataaaaaagg ccggaaaatc cggccttttg
    16201 acgcttttgc agtcttattt ttcctgaaga ccgagttttt tctccagata gtggatatta
    16261 gtaccaccat gctggaagtt ctcgtcattc atgatgcgga tctgcagatc aacgttggtt
    16321 ttgataccgt cgatgatcag ctcctgcagc gcattcttca tgcgggcaat cgccacgtca
    16381 cggttttcac cgtagcaaat cagcttaccg atcattgagt catagtacgg cggtacggtg
    16441 tagcccgcgt agatatgaga ctcccaacgt acgccaaaac cgccaggtgc gtggaaacgg
    16501 gtgattttgc ccggacttgg caggaaggtg ttcggatctt cggcgttgat acgacattcc
    16561 accgcatggc cgcgaacgtg aacttcttct tgcttgatcg acagcggttg accggcagcg
    16621 atacgcagct gttctttgat caggtcaacg ccggtgatca tttcggtaac cggatgttct
    16681 acctgaatac gggtgttcat ttcgatgaaa tagaactcgc cgttttcgaa caggaactcg
    16741 aaagtacctg caccgcgata gccgatatca acacacgctt tagcgcaacg ttcgccgatg
    16801 tagcgacgca gttccggggt aatgcccggt gctggtgctt cttcgaccac tttctggtgg
    16861 cggcgctgca tggagcagtc acgttccgcc agatagatag cgttgccctg accgtcagcc
    16921 agtacctgaa tctcgacgtg gcgaggattt tccaggtatt tctccatgta aaccatatcg
    16981 ttgctgaaag cagctttcgc ttccgcacgg gtcatggaga tggattgtgc cagttcagcg
    17041 tcgccgcgca ctacgcgcat accgcgaccg ccgccgccgc cggaggcttt gataatcacc
    17101 ggataaccaa tgcgtttagc aatggcgcgg tttttatcca tatcgtcgcc cagcgggccg
    17161 tcagaacccg gtacgcaagg aacgcccgct tttttcatcg ccgcgattgc ggatactttg
    17221 tcgcccatca ggcgaatggt ttctgctttc gggccaatga agataaagcc ggagcgctca
    17281 acctgctcgg caaagttggc gttctcggag aggaagccgt aacccggatg gattgctact
    17341 gcgccggtga tttcagcggc gctgatgatt gccgggatgt tcagataact ttttactgac
    17401 ggagcagggc cgatacagac cgtttcatct gccagtaata cgtgttttag atcgcgatcc
    17461 gcgctggagt gtacagcgac agtcttgatg cccagttctt tacaggcacg aagaatacgc
    17521 aatgcaatct cgccgcggtt ggcaataaca attttatcca gcatgttcgc ctcgttactc
    17581 gatgacgacc agcggctcgt caaattctac cggttgtcca ctttcgacca gaattgcttt
    17641 cacggtaccg gatttgtccg cttcgatctg gttcatcatt ttcatggctt caacgatgca
    17701 cagggtatcg cccacgttga ctttctgacc cacttcgatg aacgctttcg cgtccgggct
    17761 tggggtgcgg tagaaagtac caaccatcgg ggaacgtacg atgtgaccac tgatttccgc
    17821 tgctgctggc gcttccatgg aaggaacggt cgccggagcg gctgcgttag attgagctgg
    17881 ctgctgcatc attggtgcag cgtaagcttg ttgcatcaca gggaaacttg cggcaggagc
    17941 tgcacggctg atgcgtactg actcttcgcc ttcggagatt tccagttcgg agatgcctga
    18001 ttcttcaacc agctcgatca gttttttaat cttacgaata tccatgagtg ggttccgtac
    18061 tctttgttta gtgtgattgt gacaggcgtt tcaccgccgt ctgtaaagcg tatgccccga
    18121 cagctgtatg catagcgaga aattccagca ggccgggacg ccggtctatt ttgccccaga
    18181 gtgtcaatgt tagacttgac ggacattgtg cagcgatctg ctgctatcct ccggcaaaaa
    18241 acaaaatata ccttcgttgc gccaatgtca ccttttccgc agcgcaaaaa ctgcgtcagg
    18301 gaggacgagg tcgcacatta taacgatttc gtagcaattg gcagctaaat actggtctta
    18361 tcagggaaga taatcaacag ctaacatgta aataaccttc aacaccgtgt aatttgcaac
    18421 aagccgcaca attcacgaaa ttagcgccat catcgacgga acttcttata acgtaaggct
    18481 aaaagctgta aagccagccc tgcgaagatg aggggctgca atgagataat cttcacagac
    18541 cacaaataat gtagggatgc caggatcgcg acaagataga cgacattacg cagcggttgc
    18601 tgaggattac gcaattttca cttcatcgtc tggataaacg tgaatgctaa agcacgcaaa
    18661 atcaaccgcc tgacaatgct ctgcatgaaa taggtttaag tggttaattt gtaatcaaat
    18721 aaaatcagat tattcccccc attttgcaga aggtaaactg ttttgtcagg cggcagtgcg
    18781 gctatgtgat gcattcagaa tttgccgcgt gacaataaaa aagccgattt agtagcaaaa
    18841 tcggcttcgg tgagtttgca gcattcatca catactccga caagcttttt caagcatgac
    18901 ggaggttgcg ttaatgcaag gtttctaccc agggataaaa gtgaccgtta cggcggatga
    18961 atatctgtgt tgtcagagga tggtcatcct cacggaagca ttgttctgtt aactgtcgta
    19021 atgcgctgag atttgctggc tgatcattgt gggtgaaaaa cagttttcct ctggagggaa
    19081 cggtcactaa caggtcgccg cattcaggat cgggtgattg cacccaaaga tcgtcaatga
    19141 gcatcagact gggctcataa gtaccccctg cggagataac ggcaactttt ccatcgtttg
    19201 cgcctgtatc aacgatttca gccgtaactt ccggcaatat ttgcttcaaa ttactgatgg
    19261 cttgttcgcg gtcaatggtc acttcgcagc gcatcagata gcgcatggtt tcgctaccgt
    19321 cttcagcaaa caggataatt aactcatcgt taaagtcttc ccaggcaaaa tcaagcgagg
    19381 cgttgaggac ctctggatca gactcatact ggctaagctg ttggcgaacg gtatccagcc
    19441 aggctttttc tttaatcacc gggacaatac agcgcccggt cggcagcatt tctgcaagct
    19501 gtttcagcat atctgcagcc acttcttcca gcgggtctgg tgcggcttga taacgtttgt
    19561 aagcatcatc aagggtgatt tccacagttt ggtgacccgt atcaatgcgc atcaatttgt
    19621 ggtcagtcga aatcatgact ttccagtttg gatgatgttg tacaacttca tgaccaagat
    19681 gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatttca
    19741 aaatgagttc cctctcttat taatccttgc taaatggtta gttaaccttc accagcgtgc
    19801 gaccctggat ctggttatta atgatggcct cggcaaactt cggtgcctct gccagagata
    19861 tctcttttgc cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg
    19921 cttgtgcgcg gcgtgctggt ggcgtcatca ctgaatccac cccctgtaaa cggacattac
    19981 gcagaataaa tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggccg
    20041 ccacgcagcc gccgtaattc atttgcgcta gcactttcgc cagcactttg tcgccaacgg
    20101 tgtcaattgc cccagcccag agttgttttt ccagaggacg ggattcggca aactcatcgc
    20161 gagagagaat acggctggct cctaaacttt tcagatattc atgggtactt tcgcgaccag
    20221 aaacggcaac gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc
    20281 caccactggc acccgttacg acaatctcgc cgtcctgcgg gcgaacaccg gcatcttcca
    20341 gcgccatcac acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg
    20401 cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca
    20461 gcccacccca gtggttttca ccaacgcccc agccagtgag taatacctcc tgaccggcat
    20521 gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg
    20581 gaaaattacg gatgattttt cccttaccgg taattgccag cgcatctttg tagttcaggc
    20641 tcgaccagtg aacatcaacc gtgacatcgc cctccggcag gcgactttcg tccagagtct
    20701 gtaccgatgc gagagttttg ccgtcctgct gttctaaaag taacgcctgc ataagtggtc
    20761 ctcatgtgca tgatggattg gaaaataatt tctgaagact atactcgcta atggaaaagc
    20821 aaagccgatg aagcgcaata aattgcgaga taaatctgat ttgctagtat gcccgcttcc
    20881 tcactatcgg agttaacaca aggatgagat taacgacgaa attttcggcc tttgttacgc
    20941 tgctcaccgg gttaacaatt tttgtgactt tgctgggctg ttcgctaagt ttctacaatg
    21001 ccattcagta taagtttagt catcgcgttc aggcggtggc gacggcaatt gatacccacc
    21061 ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac cgaattaatg atgtcggcag
    21121 atatcgttcg tgtagacctg ctgcatggtg ataaacaggt atataccctg gccagaaatg
    21181 gaagttatcg tccggttggc accagcgatc tgtttcgcga actgagcgtt ccgttgataa
    21241 agcatccggg gatgtcgctg cgtctggttt atcaggatcc gatgggcaac tatttccatt
    21301 cgttgatgac caccgcgccg ctcacggggg cgattggctt tatcattctt atgctctttc
    21361 tggcggtacg ctggttacaa cggcaacttg ccgggcaaga attgctggaa acccgggcta
    21421 ctcgtatctt aaacggtgag cgtggctcta atgttttggg aaccatctat gaatggccgc
    21481 ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac
    21541 acagccgtct tgatacgctg atccgctctt atgccgccca ggacatgaaa accggcctca
    21601 ataaccgact cttcttcgat aatcagttag caacgttact ggaagatcag gagaaagtag
    21661 gtacccacgg gatcgtgatg atgattcgtc tgccggattt caatatgttg agtgatacct
    21721 gggggcacag ccaggttgaa gaacagttct tctctctgac gaatctgcta tcgacattta
    21781 tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt
    21841 taccgcaccg gacgttaaaa gaggcagaga gcatcgccgg tcagttaatc aaagccgtcg
    21901 ataccttgcc gaacaataaa atgctcgatc gcgacgatat gatccacatt ggtatctgcg
    21961 cctggcgtag tggtcaggat accgagcagg taatggaaca tgcagagtct gccacgcgta
    22021 atgcgggatt gcagggcggc aatagctggg ctatttacga tgactcgttg cctgaaaaag
    22081 gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat gctgagtcgc ggcggcccgc
    22141 gcctttatca aaaaccggcg gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt
    22201 gccgcatctt cgatggtaat gaagaggtta gctcggcgga gtatatgccg atggtcttgc
    22261 agtttggctt atcggaagag tatgaccgcc tgcaaatcag ccgtcttatt ccactattgc
    22321 gttactggcc tgaggaaaat ctggcgattc aggttactgt tgagtcgctg attcgcccgc
    22381 gttttcagcg ttggctgcga gatacgttaa tgcaatgtga aagatcgcaa cgaaaacgca
    22441 taattattga acttgcagag gccgatgtag gtcaacatat cagtcgctta caacctgtta
    22501 ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg
    22561 taagcaccag ttggatcaaa gaacttaatg ttgagttact caagctccat ccggggctgg
    22621 tcagaaacat tgagaagcga acggagaacc agctgctggt tcaaagcctg gtggaagcct
    22681 gctccgggac cagcacccag gtttacgcca ccggcgtgcg ttcgcgaagc gagtggcaga
    22741 ccctgattca gcgcggtgtt acaggtgggc aaggggattt tttcgcgtcc tcacagccac
    22801 ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt ttaacctgcc gtttaatccg
    22861 ttttcacgta gaataacgcg cgctgcgtct catgggagtg tgcttgtctg ctcgccagat
    22921 tgttgcagca catatgcaga tgaatgacct tacgcggttg caaacaggcg aggaatgctg
    22981 ctgatgcatt aagcctttct ggactcaggc agagatttgt aacaaaggaa acgaactgca
    23041 ctaattttca ccgtagcaga tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa
    23101 gtaagcggat tttcttttcc gccccagctt tcaggattat cccttagtat gttgaaaaaa
    23161 tttcgtggca tgttttccaa tgacttgtcc attgacctgg gtactgcgaa taccctcatt
    23221 tatgtaaaag gacaaggcat cgtattgaat gagccttccg tggtggccat tcgtcaggat
    23281 cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg acgcgaagca gatgctgggc
    23341 cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag acggcgttat cgccgacttc
    23401 ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag tgcacagcaa cagctttatg
    23461 cgtccaagcc cgcgcgttct ggtttgtgtg ccggttggcg cgacccaggt tgaacgccgc
    23521 gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag tcttcctgat tgaagaacct
    23581 atggctgccg caattggtgc tggcctgccg gtttctgaag cgaccggttc tatggtggtt
    23641 gatatcggtg gtggtaccac tgaagttgct gttatctcct tgaacggcgt ggtttactcc
    23701 tcttccgtgc gcattggtgg tgaccgtttt gatgaagcta tcatcaacta tgtgcgtcgt
    23761 aattacggtt ctctgatcgg tgaagccacc gcagaacgta tcaagcatga aatcggttcg
    23821 gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg gccgtaacct ggcagaaggt
    23881 gttccacgcg gttttaccct gaactccaac gaaatcctcg aagcactgca ggaaccgctg
    23941 accggtattg tgagcgcagt aatggttgca ctggaacagt gtccgccgga actggcttcc
    24001 gacatctccg agcgcggcat ggtgctcacc ggtggtggcg cactgctgcg taaccttgac
    24061 cgtttgttaa tggaagaaac cggcattcca gtcgttgttg ctgaagaccc gctgacctgt
    24121 gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca tgcacggcgg cgacctgttc
    24181 agcgaagagt aatcggatgc aggcagggga aatgtctgtt taccctgcct ggtctgatac
    24241 gagaatacgc ataacttatg aagccaattt ttagccgtgg cccgtcgcta cagattcgcc
    24301 ttattctggc ggtgctggtg gcgctcggca ttattattgc cgacagccgc ctggggacgt
    24361 tcagtcaaat ccgtacttat atggataccg ccgtcagtcc tttctacttt gtttccaatg
    24421 ctcctcgtga attgctggat ggcgtatcgc agacgctggc ctcgcgtgac caactagaac
    24481 ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg
    24541 gacaatacaa acaggagaac gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg
    24601 atgagcagaa aatggtgact caggttatct ccacggttaa cgatccttat agcgatcaag
    24661 ttgttatcga taaaggtagc gttaatggcg tttatgaagg ccagccggtc atcagcgaca
    24721 aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt
    24781 gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa cgatatccgc gtgattgcag
    24841 ccggtaacgg ttgtacggat gatttgcagc ttgagcatct gccagcgaat acggatattc
    24901 gtgttggtga tgtgctggtg acttccggtc tgggcggtcg tttcccggaa ggctatccgg
    24961 tcgcggttgt ctcttccgta aaactcgata cccagcgcgc ttatactgtg attcaggcgc
    25021 gtccgactgc agggctgcaa cgtttgcgtt atctgctgct gctgtggggc gcggatcgta
    25081 acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc
    25141 agatgatgcc gcaggtattg ccttcgccag acgcgatggg gccaaagtta cctgaaccgg
    25201 caacggggat cgctcagccg actccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc
    25261 cgggagggca atagtggcga gctatcgtag ccagggacgc tgggtaatct ggctctcttt
    25321 cctcattgcg ctgttgctgc aaatcatgcc ctggccggat aacctgattg ttttccggcc
    25381 aaactgggtg ttactcatct tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt
    25441 gggcacaggt tttgtgatgg gtgccatact ggatctgatc agcggctcga cgcttggcgt
    25501 acgcgtattg gcgatgagca tcattgctta cctggtggcg ctgaaatatc agcttttccg
    25561 caacctcgca ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg tggtggatat
    25621 tattgttttc tgggcagagt ttttagtgat taacgtctct ttcagaccgg aagtgttctg
    25681 gagtagtgta gtcaatggag tgctctggcc gtggattttc ttgctgatgc gtaaagtccg
    25741 tcagcagttt gcagtgcaat aaaggtttct atgacttctc tgtatttagc ttccggttct
    25801 ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc
    25861 attgaggagc agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc
    25921 gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggac
    25981 actatcgtta tcctgaacgg agaagtgctg gagaaaccgc gcgacgcaga gcatgcggtg
    26041 cagatgttgc gcaaattatc gggtcagacc catcaggtaa tgacggcagt ggcgttggcc
    26101 gacagccagc acattctcga ttgcctggtg gtcaccgatg tgactttcag aacgttaaca
    26161 gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac
    26221 ggtattcagg ggctgggtgg ctgttttgtc aggaagataa atggcagcta tcacgccgta
    26281 gtcggcttac cgctggttga aacgtatgaa ttattaagta attttaacgc actgcgtgag
    26341 aaaagggata aacatgacgg ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt
    26401 ggcgtatatt gatggcggta ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg
    26461 aatagtaggc aatatctaca agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc
    26521 ttttgtagat attgggctgg ataaagccgc gtttcttcat gcatctgaca tcatgccgca
    26581 caccgaatgt gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact
    26641 ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg gcactaaggg
    26701 ggcgcgcctg accacggata tcacgctgcc ttctcgctat ctggtattta tgccaggggc
    26761 ttctcacgtt ggggtttccc aacgaattga aagcgaatca gaacgtgaac gcctgaaaaa
    26821 agtggtcgca gagtattgcg acgagcaggg cgggtttatc atccgtaccg cagctgaagg
    26881 ggttggcgag gctgaactgg cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt
    26941 gatggagcgt aaaaagcgcc cgcagactcg ttatcagctg tacggcgaac tggcgctggc
    27001 gcagcgtgtt ctgcgtgatt tcgccgacgc cgaactggat cgcattcgcg ttgactcacg
    27061 cctgacttac gaagcattac ttgagttcac ctcggagtac attcccgaga tgaccagcaa
    27121 gctggagcat tacaccggac gccagccgat tttcgatctc tttgatgtcg aaaacgaaat
    27181 ccagcgagcg ctggaacgca aagtagaact gaaatccggt ggttatctga ttatcgacca
    27241 gaccgaagcg atgaccaccg tggacatcaa taccggagcg tttgtcggtc atcgcaatct
    27301 ggacgacacc attttcaata ccaatattga agcgacgcag gctatcgctc gccagttacg
    27361 gttgcgcaat ctgggcggga ttatcattat tgatttcatc gacatgaata atgaagatca
    27421 ccgtcgccgc gtgttgcatt cgctggagca ggcgttgagc aaagaccggg tgaaaaccag
    27481 cgttaatggt ttttcggcgc tggggctggt ggagatgacg cgtaaacgca cccgcgaaag
    27541 cattgagcac gtactatgta acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac
    27601 cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt cgtgtacacc atgcttacga
    27661 ttccgaccgt ttcctggtct atgcttcccc ggcagtagct gaagccttga aaggcgaaga
    27721 gtcacactcg ctggcggaag tggaaatttt cgttggtaaa caggttaaag tacaaattga
    27781 accgctctat aaccaggagc agtttgacgt cgtaatgatg taaacagatg ctgggccgcc
    27841 atccggcaaa gggtttttga gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat
    27901 tgccggggat attactgctt actggagccg cgctcgttgt gatcgctgcc ctgctggtta
    27961 gcggcctgcg tattgcttta ccgcatcttg atgcctggcg tccggaaatc ctcaacaaaa
    28021 tagaatccgc gactggcatg ccggtagaag ccagtcagct ctcagccagc tggcagaatt
    28081 ttggcccgac gcttgaagca cacgacatcc gtgcagaact aaaggatggc ggcgaatttt
    28141 cggttaaacg cgttactctt gcgctagatg tctggcagag cctgttacat atgcgctggc
    28201 agtttcgcga cctcactttc tggcagctgc gctttcgcac caacactcct atcaccagcg
    28261 gcggtggtaa tgatagcctg gaagccagtc acatcagcga tctgtttctt cgtcagttcg
    28321 accattttga tctccgcgac agtgaagtca gtttcctgac gccatccggt cagcgcgccg
    28381 agctggcgat cccacaactc acctggctga acgatccacg tcgacaccgt gcagaaggcc
    28441 tggtaagcct ctccagcctt accggacagc acggcgtgat gcaggtgcgc atggatttgc
    28501 gcgatgatga ggggttgtta agcaatggtc gcgtctggct ccaggcggat gacatcgacc
    28561 tgaagccgtg gctcggtaaa tggatgcagg acaatattgc gctggaaacg gcacagttct
    28621 cccttgaagg ctggatgacg atcgacaaag gcgatgtaac cggcggtgac gtctggctga
    28681 aacagggcgg tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg gtggataatc
    28741 tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt ctctattccc gacacacgga
    28801 tcacgatgga cggcaaaccc tggccgagcg gagcattgac gctggcctgg ataccggaac
    28861 aggacgttgg cggcaaagac aataaacgca gtgacgaact ccggattcgc gccagtaatc
    28921 tggagctggc aggcctggag ggcatacgcc cgctggccgc gaaactttca cctgcactgg
    28981 gtgatgtttg gcgctccaca caaccgagcg gcaagattaa cactctggcg ctggatatcc
    29041 cgcttcaggc ggcagacaag acccgttttc aggcatcgtg gagcgatctg gcctggaatc
    29101 aatggaaatt attaccgggt gcggaacact tctccgggac gctttccggc agcgtagaaa
    29161 atggtttgct taccgcgtcg atgaaacagg caaagatgcc ttacgaaacg gtattccgtg
    29221 cgccactgga aatcgccgac ggccaggcaa cgataagctg gctgaacaat gacaaaggtt
    29281 tccagctgga tgggcgtaat attgacgtta aagccaaagc cgtccatgcg cgcggcggtt
    29341 ttcgttacct gcaaccagct aacgatgaac cctggctggg tattctggct ggcatcagta
    29401 ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa cttgatgggt aaagacctgg
    29461 ttgattactt aagtggcgcg attcagggcg gagaagcgga taacgcgacg ctggtttatg
    29521 gtggcaatcc gcaactcttc ccctataaac acaacgaagg tcagtttgaa gtgctggtgc
    29581 cgctgcgcaa cgcgaagttt gccttccagc cggactggcc tgcattaact aaccttgata
    29641 ttgaactgga ctttatcaac gacggtttat ggatgaaaac cgatggcgtt aatctgggcg
    29701 gcgtgcgcgc gagtaatctc accgcagtga tccctgacta ctcaaaagaa aaactgctga
    29761 ttgacactga cattaaaggc ccgggtaaag ccgttggccc ttactttgat gagacaccgc
    29821 tgaaagattc tctgggtgcg accctgcaag aactccagct cgacggcgat gtgaatgctc
    29881 gcttacatct tgatatcccg ctgaacggcg aactggtaac cgcgaaaggt gaagtgacgc
    29941 tgcgtaataa cagtctgttt atcaaaccac tcgacagcac cctgaaaaat ttgagcggga
    30001 aattcagctt tattaatggc gatctgcaaa gtgaaccact gacagcaagc tggtttaatc
    30061 agccgttgaa cgtggatttt tccaccaaag aaggggcaaa agcctaccag gtcgcggtga
    30121 atctcaacgg taactggcaa ccagcgaaaa ccggcgttct gcctgaagcg gtgaacgaag
    30181 cattaaatgg cagcgtggcg tgggatggta aagtgggcat tgagttgcct tatcatgctg
    30241 gcgcgaccta taacgtagag ctgaacggcg atctgaagaa tgtgagcagt cacttacctt
    30301 caccgttagc caaacctgcg ggtgaaccac taccggtaaa cgttaaggtt gatggcaatc
    30361 tcaacagctt tgagttaacc ggacaggctg gtgcagataa ccatttcaat agccgctggt
    30421 tgcttggtca aaagctgacg ctcgaccgtg ctatttgggc ggcagacagt aaaacgctcc
    30481 cgccgttgcc ggaacaaagt ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt
    30541 ggctggccct gttccagaaa ggcgcggcgg agagtgtcgg tggtgcagcg agtttcccac
    30601 aacacataac gttacgtacg cctatgttgt cgctgggaaa tcagcaatgg aataacctga
    30661 gtattgtttc gcaaccgacg gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca
    30721 acgccacgct ggcgatgcgt aataacgcgc cgtggctggc gaatatcaaa tatctttatt
    30781 acaacccgag cgtggcgaaa actcgtggtg attcaacaca gtcatcacct ttcccgacaa
    30841 cggagcgcat taacttccgt ggctggccgg acgcccaaat acgatgcgca gagtgctggt
    30901 tctgggggca aaaattcggg cgcattgaca gtgatatcac catttctggc aatacattaa
    30961 cgctgaccaa tggactgatt gatactggtt tctcgcggct tactgccgat ggtgaatggg
    31021 ttaacaatcc gggaaatgaa cgcacctcgc tgaaaggaaa actgcgtggg cagaaaattg
    31081 atgccgccgc agaatttttt ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg
    31141 attacgattt acactggcgc aaagcaccct ggcaaccaga tgaggcgacg ttgaatggca
    31201 tcattcatac tcaactgggc aaaggcgaaa ttaccgaaat caataccgga catgccgggc
    31261 aattgctgcg cttattgagc gttgatgccc tgatgcgtaa gctgcgtttt gatttcagag
    31321 acacctttgg tgaagggttc tattttgact ccattcgcag caccgcgtgg attaaagacg
    31381 gcgttatgca caccgacgac acgctggtgg atggcctgga agcggacatt gccatgaaag
    31441 ggtcggtaaa tctggtgcgt cgcgacctga atatggaagc ggttgtcgca ccagagattt
    31501 ctgcgacggt gggcgtggct gcggcttttg cggttaaccc cattgttggc gcggcagtgt
    31561 ttgcagccag taaagtgctg gggccgctgt ggagcaaagt ctccattttg cgctatcaca
    31621 tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa
    31681 aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc tcatatgata atcgttgcca
    31741 aaggccaacg agccggaaca taaccgtagg ccggataggg cgttcacgcc gcatccggca
    31801 gccgtaaaaa atcctctact gcagtaacta acgagtagca aaaacgatga gtctgaacct
    31861 ggtaagtgaa caattgctag cggcgaacgg cctgaaacat caggacttgt tcgcgatcct
    31921 cggtcaactg gccgaacgtc gccttgatta tggcgatctc tattttcagt cgagctatca
    31981 cgaatcctgg gttttagaag accgcattat taaagatggt tcttacaaca tcgatcaggg
    32041 tgttggtgtg cgtgcaatca gcggtgaaaa aaccggattt gcttacgcag accaaatcag
    32101 cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga
    32161 tggcaaagta cagacgctgg gcgcggtaga gcatagcccg ttgtatacct cggtagatcc
    32221 gctgcaaagc atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc
    32281 ccgcgaagcg gacaagcgcg tacaagaagt gactgccagc ctcagcggcg tttatgaatt
    32341 aattctggtt gcggccaccg acggcacgct ggcggcggat gttcgtccgc tggtgcgtct
    32401 ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg
    32461 cggtcgtttt ggttatgagt tcttccttgc cgatctcgac ggtgaagtcc gtgcggatgc
    32521 atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc
    32581 gggcaccatg ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt
    32641 aggtcacggt ctggaaggcg acttcaaccg ccgtggcact tcagtattta gtggacaggt
    32701 cggggagctg gtggcttcag aactgtgtac cgtggttgat gacggtacga tggtcgatcg
    32761 ccgtggttcg gtggcgattg atgacgaagg tacgccaggc cagtacaacg tgctgattga
    32821 gaacggcatc ctgaaaggct acatgcagga taaactcaac gcgcgtttga tggggatgac
    32881 gccgactggc aacggtcgcc gtgaatccta cgctcatctg ccgatgccgc gtatgaccaa
    32941 cacctatatg ctgccgggta aatcgacccc gcaagaaatt attgaatccg ttgagtacgg
    33001 tatctatgca ccgaactttg gtggcggtca ggtggatatt acatccggca aattcgtttt
    33061 ctccacttca gaagcgtatc tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc
    33121 gacgttgatt ggttccggta tcgaaaccat gcagcagatt tcgatggttg gcaacgacct
    33181 gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt
    33241 gggccagcca acgctgaaag ttgataacct gactgttggc ggtaccgcgt aataatttca
    33301 gtattttcag aaggataatt aatcctttta cgtgcacgaa cggtcccctc gcccctctgg
    33361 gattagggtg aggggaaccc gttggcacag gtttgtacaa cgtaacagta taatattaat
    33421 tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt taccgacctc aacaaaataa
    33481 tccgttagcg agttgatcac gacttgtacc ttcagcggca gcttatcttt ttcggtatat
    33541 aacgcataaa ccgggcgtgg atctgactgg taacgtggca gcaggatctc cagctccccg
    33601 cgattgatct cgttgatcac ccacatcagc ggcacatagg cgatcccagc gcctgcagtc
    33661 agccagcgca ccagcgtcat cggatcatta gtcacaaatc tcccttgcgg gatcaggcga
    33721 gtcgagatcc cttccggtgc gatcagttca aattcattgt cgggtcgcac gctgtattca
    33781 agccatgaat gactacttaa atcggcgggt ttttccggta tgccgtattg tgtgagatag
    33841 cttttcgcgg cgcacaccac cattggcatc gcgcccagac ggcgggaaaa caggctggaa
    33901 tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt cggcaatcag gtcgggggca
    33961 ggaattccgg taaccagatt gacgctcaaa cctgggtatt ctttcagcat ttttgctgtc
    34021 agcccggcga gaacattttg tgccatagtt gaagaacagc caatgcgcag cgtcccgatg
    34081 ggggtgttat tgaaggcata cagttgctca tgaacatcct gcacttcatg gagcatccga
    34141 cggcagccct ggtagtaaat tctgccggct tcggtcaggc caatgctgcg tgtgctacgg
    34201 tttaacagct ttacctgcaa ctcatcttcc agttttgaca ccgtctgact gatggacgaa
    34261 acgctcatct gtagctgtct ggcggcggcg gtaaaagagc caaattcaac tactttggca
    34321 aacaccgaca tgcgttttag tcgttccatt attcactctg acttaaaagt gatttagatc
    34381 acataatata gataacagca taacagttac gctaatatat taaatatcaa tctacagcaa
    34441 tgttgctctc gcccggcttt ccatgccctt ctctgcggcg acagatgctg aaaataataa
    34501 cgcctgctct ctctatacaa ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt
    34561 tgggctgtcc ttcccaccga tattttttga attgctttta tcactggcga ttttctggct
    34621 ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt gtctggcatc cggcgttgtt
    34681 caacaccgcg ctctattgct gcttgtttta tttgatatcg cgactgttcg tttgaggttg
    34741 aagtgaaaac actaataaga aaattctccc gtacggccat cacggtcgta ttagtcattc
    34801 tggccttcat cgcaattttt aatgcctggg tctattacac cgaatccccg tggacgcgtg
    34861 acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga cgtttctgga cttatcaccc
    34921 aggtgaatgt tcatgataac cagctggtga aaaaaggaca ggtactgttc accatcgacc
    34981 agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga tgttgcttat tatcaggtac
    35041 tggcacagga gaaacgccag gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt
    35101 ctcgcgaaga gatcgatcag gccaacaacg tactacaaac ggttctgcat cagttagcga
    35161 aagcgcaggc gacccgcgat ctggcaaaac tggatcttga acgcacggtg atccgcgcgc
    35221 cagcagatgg ctgggtgacc aacctcaacg tctataccgg tgagtttatt acccgtggat
    35281 caacggcggt tgcgctggtg aaacagaact ccttctatgt actggcctat atggaagaaa
    35341 ctaagctgga aggggtgcgc cctgggtatc gtgcagagat cacgccgctt ggcagtaaca
    35401 aagtgctgaa agggactgtt gatagtgttg ccgctggggt caccaacgcc agcagcacga
    35461 gtgacgacaa agggatggcg accatcgact ctaaccttga atgggtgcgt ttggcgcaac
    35521 gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa catctggcct gcgggcacca
    35581 ctgctacagt ggtggtcact ggcaaacaag atcgcgacga aagccaggat tcgttcttcc
    35641 gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg atgggtattt tctccattgc
    35701 taaccaacat attcgctttg cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt
    35761 tggctttcac ttccagctgg aaacgccacg ctgggcagta ctgacagcgg cgattgttgc
    35821 cgccggtccg gcctttgctg cgggaggtga accgtattct ggcgctattc gctatcgtgg
    35881 ctttttgcgc atcatcggca catttattgg ctgtattgcc ggactggtga tcatcattgc
    35941 gatgatccgc gcaccattat tgatgattct ggtgtgctgt atctgggccg gtttttgtac
    36001 ctggatatcc tcgctggtac gaatagaaaa ctcgtatgcg tgggggctgg ccggttatac
    36061 cgcgctgatc attgtgatca ccattcagcc ggaaccattg cttacgccgc agtttgccgt
    36121 cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgca attatggcgg atttgctctt
    36181 ttctccgcga tcgatcaaac aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca
    36241 atatcaatta atgcaactct gtatcaagca tggcgatggt gaagttgtcg ataaagcctg
    36301 gggcgatctg gtgcgacgca ccacggcgtt acaaggtatg cgcagcaacc tgaatatgga
    36361 atcttcccgc tgggcgcggg ccaatcgacg tttaaaagca atcaatacgc tatcgctgac
    36421 gctgattacc caatcctgcg aaacttatct tattcagaat acgcgcccgg aattgatcac
    36481 tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc gcgcaggacg tccacaagca
    36541 gctcaaacgc ctacggagag ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat
    36601 ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa
    36661 cacaaaaatc aacgccaccg aagaagagat cctgcaaggc gaaccggaag tcaaagtaga
    36721 gtcagccgaa cgtcatcatg cgatggttaa cttctggcga accacacttt cctgcattct
    36781 ggggacgctt ttctggctgt ggacgggctg gacttcaggc agtggtgcaa tggtgatgat
    36841 tgcggtagtg acgtcactgg caatgcgttt gccgaatcca cgcatggtgg cgatcgactt
    36901 tatctatggg acgctggcgg cgctgccgtt agggctgctc tactttttgg tgattatccc
    36961 taatacccaa caaagcatgt tattgctgtg tattagcctg gcagtgctgg gatttttcct
    37021 cggtatagaa gtacagaaac ggcgactggg ctcgatgggg gcgctggcca gcaccataaa
    37081 tattatcgtg ctggataatc cgatgacttt ccatttcagt cagtttctcg acagcgcatt
    37141 agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt ttgctggtgc gggataaatc
    37201 gcgcgacagg actggacgtg tactgcttaa tcagtttgtt tctgctgctg tttccgcgat
    37261 gactaccaat gtggcacgtc gtaaagagaa ccacctcccg gcactttatc agcagctgtt
    37321 tttgctgatg aataagttcc caggggattt gccgaaattt cgcctggcgc tgacgatgat
    37381 tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt
    37441 tcaccgacaa atgcgccgca cagcagacca tgtgatatct gcccgtagcg atgataaacg
    37501 tcgtcggtac tttggccagt tgctggaaga acttgaaatc taccaggaaa agctacgcat
    37561 ctggcaagcg ccaccacagg tgacggaacc ggttcatcgg ctggcgggga tgctccataa
    37621 gtatcaacat gcgttgaccg atagttaagt caaaaccgac gccaaaagcg tcggtttttt
    37681 catggctata cttagcgatg aacggcagaa ctcgccgcga aacgtgacgg tggcaacaga
    37741 tgaatattta tacctttgat tttgatgaga ttgagagtca ggaggatttt tatcgcgact
    37801 tcagccaagc ctttggtctg gcgaaagata aggtacgcga tctcgactca ttatgggatg
    37861 tgttaatgaa cgatgtcctg ccgctaccac ttgagattga atttgttcat ctgggagaga
    37921 aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga tgaggcagag gaagagttgg
    37981 aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc
    38041 aatatcgtcg gacaagacga tgagggttta tttgtacagt tcagccgtag cgtgccaggt
    38101 gtcaccgcta cgagcttccg taatctggta ggccgttgcg cctttctctt ctgctttttt
    38161 gttcagcatt tcacgcatat ccattggcga agacgccaca ccacttacgg atacggtccc
    38221 gattgcttca cgattttgtg cttgtgcagc atcaatggag tcggcagcga atgcaccgaa
    38281 agatagaaca gaaagtacgc ttaatgcagc aacagtggtt ttgattttca tgatttttac
    38341 ctcgacataa tcttttagct gggcctttgt ttcgtgaccc atatcacaaa atcaagtata
    38401 cactaattat tgaactaatt aataccacgc taactgtttc agttgatact aagtgttaaa
    38461 aatgtgagtt ttaataacaa aaaagaatta aaaacacggt tatttattat
//
LOCUS       DAYPHN010000028        38129 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000028 DAYPHN010000000
VERSION     DAYPHN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.028"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            4..1950
                     /gene="yegI"
                     /locus_tag="ACG0BW_001403"
     CDS             4..1950
                     /gene="yegI"
                     /locus_tag="ACG0BW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFK7258387.1"
                     /translation="MKTNIKVFTSTGELTTLGRELGKGGEGAVYDIEEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQSYPHCAWDFLLYVARNIASSFATIHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMF
                     QQAFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGV
                     IYFIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPLGLLRRE
                     YIILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFN
                     RAKMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALQDTARERQ
                     KQKFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVID
                     WKASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQQFRLHAPART
                     MPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(1963..3315)
                     /gene="yegD"
                     /locus_tag="ACG0BW_001404"
     CDS             complement(1963..3315)
                     /gene="yegD"
                     /locus_tag="ACG0BW_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFK7258388.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPDAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPDKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3449..4306
                     /gene="alkA"
                     /locus_tag="ACG0BW_001405"
     CDS             3449..4306
                     /gene="alkA"
                     /locus_tag="ACG0BW_001405"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFK7258389.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPIAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(4344..7661)
                     /gene="dgcE"
                     /locus_tag="ACG0BW_001406"
     CDS             complement(4344..7661)
                     /gene="dgcE"
                     /locus_tag="ACG0BW_001406"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFK7258390.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLVDWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNNTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAATHSERAA
                     ISLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            7979..8620
                     /gene="udk"
                     /locus_tag="ACG0BW_001407"
     CDS             7979..8620
                     /gene="udk"
                     /locus_tag="ACG0BW_001407"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFK7258391.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8712..9293
                     /gene="dcd"
                     /locus_tag="ACG0BW_001408"
     CDS             8712..9293
                     /gene="dcd"
                     /locus_tag="ACG0BW_001408"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFK7258392.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9315..11168
                     /gene="asmA"
                     /locus_tag="ACG0BW_001409"
     CDS             9315..11168
                     /gene="asmA"
                     /locus_tag="ACG0BW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFK7258393.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTYLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11460..13043)
                     /gene="yegH"
                     /locus_tag="ACG0BW_001410"
     CDS             complement(11460..13043)
                     /gene="yegH"
                     /locus_tag="ACG0BW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFK7258394.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARITGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(13244..13393)
                     /locus_tag="ACG0BW_001411"
     CDS             complement(13244..13393)
                     /locus_tag="ACG0BW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258395.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            13701..14840
                     /gene="wza"
                     /locus_tag="ACG0BW_001412"
     CDS             13701..14840
                     /gene="wza"
                     /locus_tag="ACG0BW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFK7258396.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            14846..15289
                     /gene="wzb"
                     /locus_tag="ACG0BW_001413"
     CDS             14846..15289
                     /gene="wzb"
                     /locus_tag="ACG0BW_001413"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFK7258397.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRSLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            15292..17454
                     /gene="wzc"
                     /locus_tag="ACG0BW_001414"
     CDS             15292..17454
                     /gene="wzc"
                     /locus_tag="ACG0BW_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFK7258398.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            17547..18386
                     /gene="wcaA"
                     /locus_tag="ACG0BW_001415"
     CDS             17547..18386
                     /gene="wcaA"
                     /locus_tag="ACG0BW_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFK7258399.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLAEGI
                     RGR"
     gene            18389..18877
                     /gene="wcaB"
                     /locus_tag="ACG0BW_001416"
     CDS             18389..18877
                     /gene="wcaB"
                     /locus_tag="ACG0BW_001416"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFK7258400.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITEGFFGYEIQASATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            18874..20091
                     /gene="wcaC"
                     /locus_tag="ACG0BW_001417"
     CDS             18874..20091
                     /gene="wcaC"
                     /locus_tag="ACG0BW_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFK7258401.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKKGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFSTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            20066..21283
                     /gene="wcaD"
                     /locus_tag="ACG0BW_001418"
     CDS             20066..21283
                     /gene="wcaD"
                     /locus_tag="ACG0BW_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFK7258402.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            21294..22040
                     /gene="wcaE"
                     /locus_tag="ACG0BW_001419"
     CDS             21294..22040
                     /gene="wcaE"
                     /locus_tag="ACG0BW_001419"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFK7258403.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     IRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYRAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            22056..22604
                     /gene="wcaF"
                     /locus_tag="ACG0BW_001420"
     CDS             22056..22604
                     /gene="wcaF"
                     /locus_tag="ACG0BW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFK7258404.1"
                     /translation="MQDLSGFSVPKGFRGSNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            22631..23752
                     /gene="gmd"
                     /locus_tag="ACG0BW_001421"
     CDS             22631..23752
                     /gene="gmd"
                     /locus_tag="ACG0BW_001421"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFK7258405.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            23755..24720
                     /gene="fcl"
                     /locus_tag="ACG0BW_001422"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             23755..24720
                     /gene="fcl"
                     /locus_tag="ACG0BW_001422"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFK7258406.1"
                     /translation="MRKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLETTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            24723..25202
                     /gene="gmm"
                     /locus_tag="ACG0BW_001423"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             24723..25202
                     /gene="gmm"
                     /locus_tag="ACG0BW_001423"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFK7258407.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            25199..26422
                     /gene="wcaI"
                     /locus_tag="ACG0BW_001424"
     CDS             25199..26422
                     /gene="wcaI"
                     /locus_tag="ACG0BW_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFK7258408.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFRHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            26425..27861
                     /gene="cpsB"
                     /locus_tag="ACG0BW_001425"
     CDS             26425..27861
                     /gene="cpsB"
                     /locus_tag="ACG0BW_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFK7258409.1"
                     /translation="MAQSKLYPIVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDVCEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            28142..29512
                     /gene="cpsG"
                     /locus_tag="ACG0BW_001426"
                     /gene_synonym="manB"
     CDS             28142..29512
                     /gene="cpsG"
                     /locus_tag="ACG0BW_001426"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFK7258410.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            29567..30961
                     /gene="wcaJ"
                     /locus_tag="ACG0BW_001427"
     CDS             29567..30961
                     /gene="wcaJ"
                     /locus_tag="ACG0BW_001427"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFK7258411.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            30963..32441
                     /gene="wzxC"
                     /locus_tag="ACG0BW_001428"
     CDS             30963..32441
                     /gene="wzxC"
                     /locus_tag="ACG0BW_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFK7258412.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNCVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFMFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGLLLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            32603..33883
                     /gene="wcaK"
                     /locus_tag="ACG0BW_001429"
     CDS             32603..33883
                     /gene="wcaK"
                     /locus_tag="ACG0BW_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFK7258413.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKKHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            33880..35100
                     /gene="wcaL"
                     /locus_tag="ACG0BW_001430"
     CDS             33880..35100
                     /gene="wcaL"
                     /locus_tag="ACG0BW_001430"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFK7258414.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPQGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            35111..36505
                     /gene="wcaM"
                     /locus_tag="ACG0BW_001431"
     CDS             35111..36505
                     /gene="wcaM"
                     /locus_tag="ACG0BW_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFK7258415.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQIVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCENINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGRMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQISVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            36680..37573
                     /gene="galF"
                     /locus_tag="ACG0BW_001432"
     CDS             36680..37573
                     /gene="galF"
                     /locus_tag="ACG0BW_001432"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFK7258416.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         9349 a         9627 c        10233 g         8920 t
ORIGIN      
        1 attatgaaga caaatataaa agtatttaca tcgacaggtg aattgaccac tctcgggcgt
       61 gaactgggca aaggtggcga aggcgcggtt tatgatatcg aggagtttgt cgatagcgtc
      121 gccaagattt atcacacgcc gccacccgcc ttaaaacagg acaaacttgc ctttatggct
      181 gcgacagctg acgcgcagtt gttgaattat gtcgcctggc cgcaggcaac gcttcacggt
      241 ggacgaggcg gaaaggttat cggttttatg atgccaaaag tttctggtaa agaaccgatt
      301 catatgatct atagcccggc acatcgtcgc cagagttacc ctcattgtgc gtgggatttt
      361 ctactctatg ttgcgcgcaa tattgcttca tcttttgcta cgattcacga gcacgggcac
      421 gtcgtgggtg acgtaaacca gaatagcttt atggtaggtc gcgacagcaa agtggtgttg
      481 attgatagcg actcctttca gattaacgct aatggcacgt tgcatttatg cgaagttggc
      541 gtgtcgcatt ttacgccgcc agagctacaa accttgtcgt catttgtcgg ctttgaacgc
      601 acagaaaatc acgataattt tggccttgcg ttgctgattt ttcacgtctt gtttggtggg
      661 cggcatcctt attctggtgt gccgcttatc tctgatgcgg ggaatgcact ggagacggat
      721 attgcccatt tccgttatgc ctacgcgtca gataatcagc gacgtggttt aaaaccgccg
      781 ccacgatcta ttccgctgtc gatgttaccg ggcgatgttg aagccatgtt tcagcaggca
      841 ttcacggaaa gtggcgtggc aaccgggcgt ccgacggcaa aagcgtgggt agcggcactt
      901 gattctctac gccaacagtt aaagaaatgt accgtttcgg caatgcatgt ttaccccggt
      961 catttgactg actgcccgtg gtgtgcgctg gataatcaag gcgttatcta ttttattgat
     1021 ctcggcgaag aggtcattac caccggcggt gattttgtgc tggcgaaagt ctgggcaatg
     1081 gtgatggcgt cagtagcacc accagcattg caactgccat tacccgatca tttccaggct
     1141 gctggcaggc cgcttccttt aggcctgtta cggcgtgaat acatcattct gattgagatc
     1201 gcactgtcag cgttatcgct gttgctttgc ggccttcagg cagaaccgcg ttatattatt
     1261 ttggttcccg tgctggcggc tatctggatt attggcagcc tgacaagcaa agcgtacaaa
     1321 gcagaaatcc agcaacgtcg tgaggcgttt aatcgcgcga aaatggacta tgaccattta
     1381 gtcagccaga tccaacagtt gggcgggctg gaaggtttta tcgccaaacg gacgatgctc
     1441 gaaaaaatga aggacgaaat gctcgggtta ccggaagaag agaaacgcgc tctggcagca
     1501 cttcaggaca ccgcaaggga acggcagaag cagaagtttc tggagggatt ttttattgat
     1561 gttgcctcta ttcccggcgt tggccctgcg cgtaaagctg cgttacggtc ctttggtatt
     1621 gaaacggcgg cagacgttac ccgacggagc gttaagcaag ttaaaggttt tggtgatcat
     1681 ctgacccagg cggttatcga ctggaaagcg agttgcgaac gccgttttgt tttcaggccg
     1741 aacgaagcgg taacgcctgc agacagacaa gcggtaatgg caaaaatggc cgccaaacga
     1801 catcggctgg aatcggcgct gactgtcggt gcgacagagt tgcagcaatt ccgccttcat
     1861 gctccagcac ggaccatgcc gttgatggaa ccgttacgtc aggcggcaga aaaactggct
     1921 caggcgcagg cagatttaag ccgctgctga gccatttttc aattaacgaa acaccacttc
     1981 cgcccagcgt gccagcccgg cggtgacgga gccaaagtca tcgccgcctg caatcggaat
     2041 gcccggcaac tgttctgcca gcgctttttt aatcagcggt gaacgggcgc taccgccggt
     2101 cagatagata acatccggtt tttcctgggc gttatccagc gccagttgca cctgttccag
     2161 aatccgcgcc agtggctggt tgagggcgct ttccagccct tgctggctaa tcagcgtagc
     2221 cagttcatcg ctgataaacg gcagtgaggc gcgggtttcc gctacgcttg aaagcgcaat
     2281 tttgctctct tctgcgctac gtaccaggcg atagcttaaa cgctgacgcc agactttctg
     2341 taacagggcc actttatccg gttcgcgggc atcgcgtacc agatcgttaa gcaaacgacc
     2401 gttggcacta ctgtagaaat cactttgcgc aggtacgtcg ttgatggcaa ccgcattcca
     2461 ccacggcaga atcggcaggg cgatgccttt ttcggtttcg ccacccatgc ccagtaacgg
     2521 catcaggttt ttaaacgcca gtgcgatatc cagatcgtta ccgccaatac ggcaaccact
     2581 gtgacccagc aggctggctt cacgatcgag acgcgaacgc cactgcggcc ccatcagcag
     2641 caatgaacag tcagtcgtac caccgccgat atccaccacc agcacccgtt tttcttcctg
     2701 caaggtggct tcgtaatcca gcccagccgc gaccggctcg tactggaata ccacatcctt
     2761 aaacccggca cgctttgccg cgcgttccag aatcccttgc gcctgggtgt ttgcttcatc
     2821 gccgcccagc ccctggaagt tgatcgggcg accaatcacc gcctgagtaa tcgcatccgg
     2881 cagctgcgcc tgcgcctgct ggcgaatgtg caacatcatt gcgcagacca gatcctcaaa
     2941 cagcgctacc tgctgcggtt ttaagccgct ggcaccgagg aatgattttg gtgatttcac
     3001 aaaccacacc tcttctggat catcaatgta ctgtgccagt gaggagagac cgaactgcac
     3061 gcttttcgcc gtaacatcga tatcttcttc gcggttataa cgaatcgccc gacgcagcag
     3121 cgcctgcgtt tcatcgtcgt ctgccggaac atcatgatgg cggtacagcc attcgcttac
     3181 cgcttcacgc gttggcgcgc aaagcattga aggcagcagc gtgctgtcgt tttccatttt
     3241 cagtaattgc ggcttaccgt cgcgcatgac cgccactgaa cagtttgctg taccgtaatc
     3301 aaaaccaata aacacgaaat aatccccatg ccggtgaaga aggggcgtga ctttagcgaa
     3361 atgttgccgt cgcgacaacc ggaatatgaa agcaaagcgt agcgtctgaa taacgtttat
     3421 gctgaaagcg gattaacaag gagatgcgat gtataccctg aactggcagc cgccgtatga
     3481 ctggtcgtgg atgttgggat ttctcgccgc ccgtgcggtg aacggtgtgg aaacggtcgc
     3541 tgacgattat tatgcccgta gtctggcggt gggcgaatat cgcggcgtgg tgactgctat
     3601 tccggatata gcccgccata ctctgcacat aaatttaagt gcaggtttag aacctattgc
     3661 cgcagagtgt ctggcgaaaa tgagccgcct gtttgatctg caatgtaacc cgcagattgt
     3721 taacggtgcg ttgggcaagt taggcgcggc gcggcccgga ttgcgtttac ccggttgtgt
     3781 tgatgctttt gagcagggcg tgcgggcgat tttaggccaa ctggtgagcg tggcgatggc
     3841 ggcaaaattg accgccagag tggcacagct ttatggcgaa cggctggatg attttccgga
     3901 ttatgtctgc ttccccacgc ctcagcggct ggctgcagcc gacccgcagg cattaaaagc
     3961 gttaggtatg ccgttgaaac gggcagaggc actgattcat ctggcaaatg cggcgctgga
     4021 gggcacctta ccaatgacaa ttccgggcga cgtagagcaa gcgatgaaaa cgctgcaaac
     4081 ttttccgggt atcgggcgct ggacggcgaa ttatttcgcc ttacgtggct ggcaggcgaa
     4141 agatgttttc ctgccggatg attatctgat taaacagcga tttccgggaa tgacaccggc
     4201 acaaatccgc cgttatgccg aacgctggaa gccctggcgt tcttatgctt tgttgcatat
     4261 ctggtatacg gaaggctggc aaccagacgg aacggacgaa ttgtaggcct gatacgccgc
     4321 atcaggcgat ggggaagcac gccttagtta atcgcgaaat aactgctatt caccagcaaa
     4381 tccagcggtt gggcatcggc aatcacatcg ccataaatca gatcgacgcc gatgccagaa
     4441 agcgtatcca tcaccagggg taaaactact ggcccggcga tggtcttcat cccgagccgc
     4501 tgagcgtgcc cctgaatgat cgtaatcagc atctcatcca tcaaatttcc ctgcacgttg
     4561 gcgcataact caccatcaag tagcaggtaa tctgccatat ttgctttcag cgagttgaag
     4621 atttgcagat cgcggcccac ctgactgaat acgatccgac atcccgccag ccgcagtttt
     4681 tgcacgcttt ctgcgtgatc taaaatcgcg tctgccggaa taatcagatg taataaccgt
     4741 ggtggtagag ggctattttc cagctgctca attagttcat tcaccagcgt ggcgctactc
     4801 aaaccggcaa cggaaagggg gagggcgata cttaagcctt tactggcaat agcttttgcg
     4861 gcctgctgga aaaaatcgtg gaatacccgt cggtcaagag catggctaag tgccggatcg
     4921 ctgaagctac gacgaaatgt ttgttcatca ataatctcgc cttcgcaact ccagagctta
     4981 agtgaaatca gccacaaatt acgtgcttcc gggatccgcg gcgaagcgac accgtgggcg
     5041 atcatcatca actgattttc tttaatcatc cgccactgtt catcaagcga tatcgccgcc
     5101 cgttcgctat gtgtggcagc ttgctgcggt tcgtaaatcg tcacccggcc ccgtccacca
     5161 tttttggagg cataacaggc gatatcagcc tgcgacatca cttcagctgc ctgatgattg
     5221 ttgtcatcaa tcaaggtaat cccggcactg gcacctaccc gatgtactcg tccttcccag
     5281 ataaagtgat agtcattaac ggcgctgata atgcgtgtag cgataaaacg cgcactttcg
     5341 acattacagt ctggcagcag cagaccaaat tcatcgccac cgagtcgcgc cagcacgtcg
     5401 ctggagcgca gcatactcag catcaatgac gccagttcgc gcaacaaagc gtcgccagcg
     5461 gcgtgcccgg cgctgtcatt caccgcttta aagcgatcaa gatcgataaa caccagtgca
     5521 tgtcgctgat gtgtattgtt taccgtttgc agcaggatac gcagttgctt ctcaaaactg
     5581 gcgcgattgg cgagatgcgt cagtgcatca tgggaggcgc tgtagctcag ctggcgcagc
     5641 attttgcgtg attcggtgac gtcctgaatc accagaaccg agcctatatt gctgccgtcc
     5701 agagtactta acggcgtaat actataatgc acgtcgtagc tgccgccgct ccgacagtgc
     5761 aacaccacat cttgttcaat cgcggaacgt gaggtgtcgg cactgtaaat gttctccatt
     5821 aatggtccgt tgtcgccaaa agtaatatgc aacaccgtca ggagcggaac acctaatgct
     5881 tcttcctgcg tccagccgct catcttctcc gccaccggat tcataaaggt aattttcatc
     5941 gccatatcaa tacagaccac ggcttcaccg atggagtcca gcgtaatgtg caggcgctct
     6001 ttttcctgaa acaatgcctc gttaagctgt ttcacttcgg tcatatccat attaatgccg
     6061 agcaggcgtt cgacttcgcc ttctttattc agtacccggt tagcgagggc gcggatatgg
     6121 cgaatgccgt ctttcacggt aatgcgaaat tccagtttaa agggagagcg tgattgcaat
     6181 gaatcgcgaa tcactttttc ggcgtgctgg cgatcttccg gcagcacgca ctcgtaccac
     6241 acctgccagt tcggtttgat atgcggagga atttcataca gctcgaacat ccgcttatcc
     6301 cagctaaaaa tattcggctt tagctcccac tcccagatgc caatcccgcc cgcttcgtta
     6361 gccagcgtga tgcgctccat cagttgctga ttcacctgtt cggtgcgttt tagctcgtta
     6421 atgtcttcaa tctgagcgat aaaatagagc ggcgtgccat ccgtgtggcg taccagtgag
     6481 acggcaagca acgcccagac aacatcgcca ttgcggttgt agtagcgttt ttccattgaa
     6541 taggtgttta tttcaccgct gatcagcttt tcaacctgtt gcagatcttt attgagatcc
     6601 tccggccagg tcagttgctg aaaggtgagt ccgcgcagtt cttcctgact gtaaccgaga
     6661 aactggcaga gtgctttgtt ggattgcagc cattgccctt cggtgcccac taatgccatg
     6721 ccgatggcgg aatattccat cgcgttacga aaacgggttt cgctttcgga aatgtgtttg
     6781 cgttccgcac gaaaggcata catcaccatg gtcatgatgt tggcgggcag caggatcagc
     6841 aaaaacggta gccacggcat atggctcatc aggtatgtac gcggcgtagc aagcagggag
     6901 ggatcagcgg ccatcatcag tgacaccatc atcaccgtgg taaggaagat caaaaaggct
     6961 tccattcgtg gcaggcgcac ggcgctccac atcaacagca caataatgaa agtaaaaggc
     7021 cacggcagat acagcatcga aagccagctt aacgtcagtg tgatggccaa cgtgagcagc
     7081 gactcaaaaa gcaaccgtgg gttgcgatgg cgcagcagat agtgtggttt aaataacaat
     7141 cccagtggca ccagtgccag tgcgccgatg gattctgaca gtacccatat caaaaatgcc
     7201 ctgagaggat cgtctccggg cgtcagcagg ataaccagaa caccccctaa cagaggtgga
     7261 acaatggcgc tgccgagtgc cagacgcagc cagtcaacca gattttgcaa ggggttatac
     7321 cacggcagca atttacgcag tagcactgct ccgaccacgg cttcaacaat attgatggtc
     7381 gtccaggtca tgttcagcga gctggtggaa aaaagcagga tggatgcggc gatatttccc
     7441 agcgagcagc tcagcgcaat ccccggccac atgcgcccgg catggcgata aaacgccacc
     7501 atcatgatgg acgtcgggaa ccacagtggg gcgagttggg tgccaaactg cgaaagctca
     7561 agcgagaaaa gggtaaagat aaacgagact aaacctaaac tgaccaggtg aagcagcggg
     7621 tggggcaggg caattaatac atgctgtgat tgtttgctca ttaccgcttt atccgcaaga
     7681 ccaggtcgcc agtattcgtt tctgtgttta tgctagtaca aaagttttac attttatatg
     7741 cgaattgctc ataaagtgac gtaaaggcgg ataatttgcg caacggcgta taacattttt
     7801 taccttgcat taaaactgaa caacgtaatt tgccaccggg aattattcgt caacaattac
     7861 tctgagcgcg cggcaacgct attcgactgg tatcaggcgg atgaaatccc tataattgcc
     7921 gcgtttggcg cttcgtcgcc cccttcctaa catccaggtt aatcaggtcg ctaaatatat
     7981 gactgatcag tctcatcagt gcgtcattat cggtatcgct ggcgcatcgg cttccggcaa
     8041 gagtcttatt gccagtaccc tttatcgtga attacgtgag caagtcggtg atgaacacat
     8101 cggcgtaatt cccgaagact gctattacaa agatcaaagc catctgtcga tggaagaacg
     8161 cgttaagacc aactacgacc atcccagcgc gatggatcac agcctgctgc ttgagcattt
     8221 acaagcattg aaacgcggct cggccattga cctgccggtt tacagctatg ttgaacatac
     8281 gcgtatgaaa gaaacggtga cggttgagcc gaagaaagtc atcattctcg aaggcatttt
     8341 gctgctgacg gatgcgcgtt tgcgtgatga acttaacttc tcaattttcg ttgatacccc
     8401 gctggatatc tgcctgatgc gccgcatcaa gcgtgacgtt aacgagcgtg ggcgttcaat
     8461 ggattcagtg atggcgcaat atcaaaaaac cgtgcgcccg atgttcctgc aattcattga
     8521 gccttctaaa caatatgcgg acattatcgt gccgcgcggc gggaaaaacc gcatcgcgat
     8581 cgatatattg aaagcgaaaa taagtcagtt ctttgaataa gcttgataaa ttgtgtaccg
     8641 ttcagtgata acctggtatg cccttgacgt aattggcgtt aagggagtga tgcacgaaag
     8701 gagaaaatgc catgcgtctg tgtgaccgag atattgaagc ctggcttgat gaaggccgtt
     8761 tgtcgatcaa cccacgtccg ccagtggagc gtattaatgg cgcgacggtg gatgtacgcc
     8821 tgggcaataa atttcgtacc ttccgtggtc acacggcagc gtttatcgat ctgagcggtc
     8881 ccaaagatga agtgagcgcc gcgcttgacc gcgtgatgag cgatgagatc gtactcgatg
     8941 aaagcgaggc gttttatctt cacccaggag agctggcgct ggcggtgacg ctggagtcgg
     9001 tgacgctgcc agccgatctg gtgggatggc tggacggtcg ttcctcactg gcgcgtctgg
     9061 ggcttatggt gcacgtcacc gcgcaccgta tcgatccggg ctggtctggc tgcattgtgc
     9121 tggagttcta caactccggt aagttgccgc tggcgctgcg tccgggcatg ttaattggtg
     9181 cgctgagctt tgagccgctt tccggcccgg cggcgcgacc ttacaatcgc cgtgaagatg
     9241 cgaaatatcg caaccagcag ggcgcggtag ccagccgaat cgataaagac taattcaggc
     9301 ccattgagga taccatgaga cgatttctga cgacgctgat gatactcctg gtcgtgctgg
     9361 tggccgggtt atctgcgtta gtgttgctgg tgaatccgaa tgatttccgc gactatatgg
     9421 tcaagcaagt tgctgcacgt agcggttatc aattgcagct cgacgggcca ctgcgttggc
     9481 acgtctggcc gcagcttagt atcctctccg ggcgaatgtc tctcaccgcc cagggcgcga
     9541 gccagccact ggttcgcgcc gacaacatgc gtctggacgt ggcgctttta ccactactga
     9601 gtcatcaact gagcgttaag caggtgatgc taaaaggggc agtgatccaa ctgacgccgc
     9661 agacggaagc ggtgcgcagt gaagacgctc cggttgcacc gcgcgacaat accttgccgg
     9721 atctgtcaga cgatcgcgga tggtcgtttg atatatccag tcttaaggtg gcggacagcg
     9781 tgctggtgtt ccagcatgaa gatgacgagc aggtgacaat ccgcaatatc cgtctgcaaa
     9841 tggaacaaga tccccaacat cgtggctcat ttgagttctc cgggcgggtt aatcgcgatc
     9901 agcgcgatct cacgatatcc cttaacggta cggtagatgc ttctgattat ccgcatgatt
     9961 taacggcggc tattgaacaa attaactggc agttgcaggg tgcagattta ccaaaacaag
    10021 gtattcaggg gcaggggagt ttccaggccc agtggcagga gtcacataaa cgcctttcat
    10081 ttaaccaaat tagtttgacc gccaatgaca gtacgctgag cgggcaagca caggtcacgt
    10141 tgacagagaa accggaatgg cagctgagac tgcaattccc gcaactgaat cttgacaacc
    10201 tcatcccgct taatgaaaca gcgaacggtg aaaacggtgc cgcgcagcag gggcagagcc
    10261 aatcaacgtt gccgcgcccg gtcatttctt cgcgtattga tgaaccggcc tatcagggac
    10321 tgcaaggctt tacggctgat attttgttgc aggccagtaa cgtgcgctgg cgcggaatga
    10381 attttacaga tgttgccacg caaatgacca acaagtcggg tttgctggaa attactcaac
    10441 tgcagggcaa gcttaacggt ggacaggttt cactgccggg cacgctggac gcgacatcaa
    10501 taaatccgcg gataaacttc cagccacggc tggaaaacgt tgagataggc accattctga
    10561 aggcgtttaa ctatccgatt tcgttgaccg gaaagatgtc actggctggt gatttctccg
    10621 gtgctgacat agatgccgac gcattccgcc acaactggca aggacaggca catgtcgaaa
    10681 tgaccgacac acgcatggaa gggatgaact tccagcagat gattcagcaa gcggtagaac
    10741 gtaatggtgg tgatgtgaag gccgctgaaa acttcgataa cgtgacgcgt cttgaccgct
    10801 ttaccaccta tttgacgttg aaggatggcg tcgtgacgtt aaacgacatg caaggtcaat
    10861 cgcctgtgct ggcgctttcc ggggctggta cgttgaatct ggcggagcaa acctgcgaca
    10921 cccagtttga tattcgggtc gtgggtggat ggaacggaga aagcaaactg attgatttcc
    10981 tcaaagaaac gccagtaccg ctgcgggttt atggcaactg gcagcaactc aattacagcc
    11041 tgcaagtgga ccagctgctg cgcaaacatc tacaggacga agcgaaacgt cgcctgaatg
    11101 actgggccga gcggaataaa gattcccgca atggcaaaga tgtgaagaag ttgctggaga
    11161 agatgtagtt taccaggtga cctgtcctgt aggcctgata agacgcgtaa agcgtcgcat
    11221 caggcgttcg tggcggcacc attatattct gaatatgtaa gatcgaaggc tgttccgttc
    11281 acttgatttc ctcgctacta tggaacgctc aagccggtga acgtgccagg gtggctcaat
    11341 ctccaccacc ctggcaaccc ggacccacac ccccgctgtg atgtccatgt accacagaca
    11401 acgcttcatt gctgaagtta taaggcgtta cgcagcattg ggtaaaatgg ccgataccat
    11461 cacacctcgt actccatctc gccatcctta tgcagcggaa taatctgcac cttctgcacg
    11521 cgatggcttt ccacctgcaa cgttttgagc aagtaatccc ctacctgaac ttcttcgcca
    11581 ggttttggaa tacgctgcaa atattccatc agcagcccgg caatggtgtg atattcgcgt
    11641 ttctcatcca gcggtagcgg tacatattgc accagatctt ccagtggcat atgaccattc
    11701 gccgtccagg aaccgtcggc gtttttctga atatcatggc gggcgtcgat ctcttccact
    11761 tcgttcggca agttaccggc aatagtttcc gtgacgtcac ttaatgtcac aatcccttcc
    11821 accgagccaa actcatccac cacaaaagca aagtgtgtgc gggcattacg gaactgctcc
    11881 agggcaggta acaacggcaa ggtttccggg aacaccagcg gctggcgaat caacacccgc
    11941 aggttgagcg gttcgccgcg cagtgactgt tgtagcaggt cgataacgtg aacaacaccg
    12001 agcaaatctt ctgcgtcatc accgtcggta accaccagcc gcgtatgctg atttcgctcc
    12061 agcaactggc ggatctcgtc ttccggcgcg ttgagatcga tatgctcaat atcgtggcgc
    12121 gacgtcataa tgctgctgac agtacgctgg ttaagattaa gtacccgctc aatcatccgc
    12181 cgttcctgcg gattaaagat ctgctggtta ccatgatcca ccagcatgga cgcggtttcg
    12241 gcgtccagtt ccgcatcttc tttttgcccg ctaagcaggc gcattaccgc ttcggtagtc
    12301 cgctggcgca gcgtctggtt agcggaaaga aaacgtcgcc ggttaaagat agccaactga
    12361 ttgagcgcct cgatcatcac cgagaaacca atagcagcgt acaggtagcc tttcggaatg
    12421 acgaagccga aaccttctgc caccaggcta aagccaatca tcaacaggaa gcttaagcag
    12481 agaataacga tcgtcgggtg actgttaaca aattgcgtta acggcttgct ggccatcaac
    12541 atcaggctga tagcgataac aacggcggcc atcatgacca gtaaatggtc gaccatcccg
    12601 acggcggtaa tcaccgagtc gagtgagaag atggcgtcca gtaccacaat ttgtgtcacc
    12661 accccccaga acttcgcacc tttgcgttgc gtggggttat tgctgtcttt cccctccagc
    12721 cgttcgttca gctccatcgt ggctttgaac agcaggaaga aaccaccaaa cagcatgatt
    12781 aagtcgcggg cgctaaaggt aaacgagcgg aagctgaaca gcggttgagt cagggtgacc
    12841 agccaggaga tagacgccag cagtaacagg cgcattaaca tcgccagcag cagcccggta
    12901 atccgtgcgc ggtcgcgctg cttcggcggt agtttttcgg cgaggatggc aataaagacc
    12961 aggttatcaa tgccgaggac cagttcgatc acaatcagcg tgattaaccc agcccagata
    13021 gacggatcgg caatccattc catagtaagt acaaaaccta ttcgttgtat gacaaatgtc
    13081 acaattcgat catggataaa tcggggcaaa aatgcaatgt tgcagcacta tttatctcat
    13141 attatttgag ggtaataacc tcacattatc cctgaattaa aagtggtaat aataaaacat
    13201 tatttaagaa atatcgcatg tggagacact atattaaata ctgtcaacct aaagaaactc
    13261 ctaaaaacca tattgaatga cacttaatat aattcttaaa aatagccaat tgccgaattg
    13321 ttaccttgcc tgctattccg ttagctgtaa cacttcctcc tgcattattg gagagccaat
    13381 attcaattac cataatgtca ccttatattc ggaagttaat ctccgtaata accctttctc
    13441 atcaacgact gcatgctgag agggtgtgaa aaattaaact aaatcagtgt attggtagct
    13501 aaaagccagg ggcggtagcg tgtctggatg cctgaaagac cagtctgaat tattctgtca
    13561 atagcctgcg gatgaaatca atttttttag gactgatgcc agttaaattt ttattcaatt
    13621 aactgcaagg taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct
    13681 gccaaagtga taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt
    13741 cagtaacctt gataagcggt tgcacagtac ttccgggcag caatatgtcg acgatgggta
    13801 aagacgtcat caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc
    13861 cgttgacccc gcgcctgatt gaccaattac gtccacgacc caacgtagcg cgccccaata
    13921 tgacgctgga aagtgagatc gcgaattacc agtatcgcgt cgggccgggc gacgttctta
    13981 atgtcactgt ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca
    14041 gcgacaccgg caactgggta cagcctgacg gcaccatgtt ctacccgtat atcggcaaag
    14101 ttcacgtggt ggggaaaacg ctcgctgaaa tccgcagtga tattaccggg cgcttagcga
    14161 cgtacatcgc tgacccgcag gtggacgtta atatcgccgc cttccgctcg caaaaagcgt
    14221 atatctcagg tcaggtgaat aaatccggtc aacaggcgat caccaacgta ccactgacca
    14281 ttctcgatgc catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg
    14341 tgctaacaca caatggtcgt gaagaacgca tttctttgca ggcgctgatg caaaacggcg
    14401 acctcaatca gaaccgactg ctttatcctg gcgatattct ctacgtgccg cgtaatgatg
    14461 atctgaaagt atttgtgatg ggtgaagtta aaaaacagag caccctgaaa atggacttta
    14521 gcggcatgac cctgactgaa gccctgggca atgctgaagg catcgacatg accacctcca
    14581 acgccagcgg catctttgtc attcgtccgc tgaaaggcga gggcgggcgt aacggcaaga
    14641 ttgccaatat ctaccagctg gatatgtccg atgccacgtc gctggtgatg gcgacagaat
    14701 tccgcctgca accttatgac gtggtgtatg tcaccaccgc cccggtttcc cgctggaacc
    14761 gtctgatcaa tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca
    14821 gcgacattca taactggtaa tcgtcatgtt taacaacatc ttagttgtct gtgtcggcaa
    14881 tatttgccgt tccccgacgg cggaacgctt actgcaacgt tatcacccgg agctgaaagt
    14941 ggagtccgct ggactcggcg cgctggtcgg taagggcgct gatcctaccg ctatcagcgt
    15001 cgccgcagaa catcaactgt ctctggaagg tcactgtgca cggcaaatca gccgcagcct
    15061 gtgtcgcaac tacgacctga ttttgaccat ggaaaagcgc catatcgaac gcttatgcga
    15121 gatggcacct gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga
    15181 aatccccgat ccgtatcgca aaagccggga aacgtttgca gcggtgtaca cattacttga
    15241 acggtctgcc cgccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa
    15301 aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg
    15361 gtcggcaccg tcattgaagc gcgctggtgg gtaattggca ttaccgccgt attcgccctc
    15421 tgtgccgtgg tttacacctt cttcgccacg ccgatataca gcgccgacgc actggtacaa
    15481 atcgagcaaa gcagcggcaa ttcgttagtg caggacattg gttcggcatt agccaacaaa
    15541 ccgccagcat cggacgctga gatccagttg attcgttcgc gtctggtgct tggtaaaacg
    15601 gtggacgatc tcgacctcga tattgcggtg agcaaaaaca cgttcccgat tttcggtgcg
    15661 ggctgggatc gtttgatggg acgccagaac gagacggtga aagtgaccac ctttaaccgc
    15721 ccgaaagaga tggcggatca agtgtttacg cttaatgtgc ttgatgacaa aaactacacc
    15781 ctgagcagtg atggcggctt tagcgcccgt gggcaagcgg gccagatgct gaaaaaagaa
    15841 ggcgtcacgc tgatggttga agctattcac gccagcccgg ggagtgagtt taccgtcacc
    15901 aaatactcca cgctggggat gatcaaccaa ctgcaaaaca gcctgacggt aacggagaac
    15961 ggcaaagacg caggcgtact gagcctgact tataccggtg aagatcgcga acagatccgc
    16021 gacattctta acagcatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg
    16081 gaagcgtcga aaagcctcgc cttcctcgcg caacagttac cggaagtacg tagccgcctt
    16141 gatgttgccg aaaacaaact gaatgccttc cgtcaggata aagattctgt tgatctgccg
    16201 ctggaagcaa aagcggtgct cgattcgatg gtgaacatcg acgcgcagtt gaacgaactg
    16261 acctttaaag aggcggaaat ctccaagctg tacaccaaag ttcatccggc gtaccgcacg
    16321 ctgctggaga aacggcaggc gctggaagac gaaaaagcca aacttaatgg tcgcgtaacg
    16381 gcgatgccga aaacccagca ggaaattgtc cgtctgaccc gcgatgtcga gtctgggcag
    16441 caggtctata tgcaactgct gaataaagag caggagctga aaatcaccga agccagcacc
    16501 gtcggcgatg tgcgcattgt tgacccggca atcacccagc ctggcgtgct gaaacccaag
    16561 aaagggctga ttatcctcgg cgcgattatc ctcggcctga tgctctctat cgtgggtgtg
    16621 ctgctgcgct cgttgtttaa tcgcggcatc gaaagcccgc aggtgctgga agaacacggt
    16681 atcagcgtct atgccagcat cccgttgtcg gaatggcaga aagcgcgcga tagcgtcaaa
    16741 accatcaaag ggattaaacg ctataaacag agccagctac tggcggtggg gaatccaacc
    16801 gatctggcga ttgaagccat ccgcagtctg cgtaccagtt tgcacttcgc gatgatgcag
    16861 gcgcagaaca atgtgttgat gatgaccggg gttagcccgt caatcggtaa aacctttgtc
    16921 tgcgccaacc tggcggcggt gatcagtcag accaataaac gcgtgttgtt gatcgactgc
    16981 gatatgcgca aaggttatac ccacgagctg ttgggcacca ataacgtcaa tggcctgtcg
    17041 gaaattctga ttggtcaggg cgatattact accgctgcta aaccgacctc tattgccaaa
    17101 tttgacctga tcccgcgcgg tcaggtaccg ccaaatcctt ctgaactgtt gatgagcgaa
    17161 cgctttgccg aactggtgaa ctgggcgagt aaaaactacg acctggtgtt gattgatacg
    17221 ccgccgattc tggcagtgac cgatgcggca attgttggtc gtcatgtcgg aaccacgtta
    17281 atggtggcgc gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt
    17341 gagcaaaacg gtattccggt gaaaggggtg attctgaact ccatcttccg ccgcgccagc
    17401 gcgtatcagg attatggcta ttacgaatac gaatataagt cggatgcgaa ataacgacgc
    17461 ctgcattcgc accgccccgt aggccggata aggcgttcac gccgcatccg gcaagcaaac
    17521 cagctcataa gtcgggagaa caacctatga aagacaatcc gctgatctca atctatatgc
    17581 cgacctggaa tcgccaacaa ctggcgattc gggcgataaa atcggtcctg cgccaggact
    17641 acaacaactg ggagatgatc atcgtggatg attgctccac ctcctgggaa cagctgcaac
    17701 agtacgtcac cgccctcaac gatccgcgta ttacttacat tcacaacgac ataaacagcg
    17761 gggcgtgcgc ggtacgtaac caggcgatta tgctggcgca aggggaatac atcaccggga
    17821 tcgatgatga tgatgaatgg acgcccaacc gcctgagcgt cttcctcgcc cataaacagc
    17881 aactggtcac acacgccttt ttgtacgcta acgactacgt ttgccagggc gaagtctatt
    17941 cccaaccagc aagtttgcca ctgtatccca aatcgccgta ttcacgccgc ctgttctata
    18001 aacggaatat cattggcaat caggtcttta cctgggcgtg gcgtttcaaa gagtgtttgt
    18061 ttgataccga actgaaagcg gcacaggatt acgacatctt cctgcggatg gtggtggagt
    18121 atggcgaacc gtggaaagtg gaagaggcga cgcagatcct gcatatcaat cacggcgaaa
    18181 tgcagatcac ctcgtcaccg aaaaaattct ccggatactt ccacttttac cgcaagcaca
    18241 aagacaaatt tgaccgcgcc agcaaaaaat atcagctgtt taccctctac cagatccgca
    18301 ataaacgcat gacctggcgc accttgttga cgctgctatc ggtgcgtaac ggcaaacgtc
    18361 tggctgaagg aatacggggg cgttaaggat gctggaagat ctgcgcgcca acagctggag
    18421 tttacgcccg tgctgcatgg tgcttgccta tcgcgtcgcc catttttgtt cagtgtggcg
    18481 caaaaagaac gttctcaaca atctgtgggc ggctccgctg ctggtgctgt atcgcattat
    18541 caccgaaggc tttttcggtt atgaaatcca ggcctccgca accattggtc gccgtttcac
    18601 tatccatcac ggttacgccg tagtgatcaa taaaaacgtg gtagcggggg atgatttcac
    18661 cattcgccac ggcgtcacta tcggcaatcg tggtgccgat aacatggcat gtccacacat
    18721 tggcaacggc gtcgaactcg gtgccaacgt cattattctt ggtgatatca cgcttggtaa
    18781 caacgtcacc gtgggcgcgg gcagcgtggt gctcgactct gtcccggaca acgcgctggt
    18841 ggtgggagaa aaagcgcgag tgaaggtaat taaatgaata ttctgcaatt taatgtgcga
    18901 ctggcggaag gcggggcggc aggtgtggcg ttagatctcc accagcgtgc gctgcaacag
    18961 gggctggcgt cacattttgt ctacggctac ggcaaaggcg gcaaagagag cgtcagccat
    19021 cagaactatc cgcaggtcat caaacatacg ccgcggatga ccgcgatggc gaacattgcc
    19081 ctgtttcgtc tgtttaatcg cgatctgttc ggcaatttca atgagttata tcgcaccatt
    19141 actcgcacac cgggtccggt ggtcctgcat tttcatgtgc tgcacagcta ctggttgaat
    19201 cttaagagcg tggtgcgctt ttgcgaaaaa gtgaaaaacc acaaaccgga tatcactttg
    19261 gtctggacgt tacacgatca ctggagcgtt accggacgct gcgcctttac cgacggttgt
    19321 gaaggctgga aaaagggctg ccagaaatgc ccgaccttaa ataattatcc gccggtgaag
    19381 attgatcgcg cacaccagct ggtggcgggc aaacgccagt tattccgtga gatgctggcg
    19441 ctgggctgtc agtttatttc ccccagccag catgtggctg atgctttcaa tagtctgtac
    19501 ggtccagggc gttgccggat tatcaataat ggcattgata tggcaaccga agcgattctg
    19561 gcggacttgc ctccggtgcg cgaaacccag ggcaagccga aaatcgcggt ggtggcgcat
    19621 gatctgcgtt acgacggcaa aactaaccag caactggtac gcgagatgat ggcgctgggc
    19681 gacaaaatcg aactgcatac ctttggtaag ttctcgccgt tcaccgctgg caacgtggtt
    19741 aatcacggct ttgaaactga caagcgcaag ttgatgagcg cgctcaatca gatggatgcg
    19801 ctggtattca gttctcgcgt cgataactac ccgctgattt tgtgtgaggc gctatcgatt
    19861 ggcgtgccgg tgattgccac ccatagcgac gcggcgcggg aagtgttgca aaaatccggc
    19921 ggtaaaaccg tcagcgaaga agacgtgctg caactggtgc agttaagcaa accggaaatc
    19981 gcgcaggcga tatttagtac cacgctggct gagttcagcc aacgcagccg cgccgcctac
    20041 agtggacaac agatgctgga ggagtatgtc aacttctatc agaatctgta gctacctgct
    20101 gctgccgctg atttacctgt tggttaacgt caaaatcgcc cagcttggcg aaagtttccc
    20161 catcaccatc gtcacttttt tacctgtctt gttactgctg tttttagaac gcatcagcgt
    20221 taaaaaattg atgattgcct tagggattgg cgcgggactc acggcgttta actacctgtt
    20281 tggtcagtcg ttggatgcca gtaaatacgt cacttcaacc atgctgtttg tctatattgt
    20341 gatcattatt ggcatggtgt ggagtattcg ctttaaaacg atttcgcccc acaaccaccg
    20401 caagatatta cgcttttttt atctggtggt cgggttggtg gtggcgctgg cggcggtgga
    20461 gatggcgcaa attatcctca ccggtggcag cagtattatg gagtcgattt cgaaatatct
    20521 gatttacagc aacagctatg tgctgaattt catcaaattc ggcggcaagc gcacgacagc
    20581 actttatttc gaaccggcat ttttcgctct ggcattaatc tcaatttggc tcagcatcaa
    20641 acagtttggt atcaaaaccc ctaaaaccga tgctatgatt ctcgcaggga taatattatc
    20701 cggatcgttt tcaggggtta tgacctttat cctgttttat ttgctggagt gggcatttca
    20761 atatctgaat aaagaggcga ttaagaaaaa gttaccgtta gcattgattt ctctggctgt
    20821 attcctggtt ggtgtggtaa ttgcatttcc ttatatttcc acccgtctgg gcgatttagg
    20881 tacggaagga tcgtcatcgt attatcgtat tgtcggtccg ctggtgatgg tcggttattc
    20941 tttgacccat attgacggtg tagtcagatt tggctcactt tatgaatatg tcgcatcatt
    21001 cggaatattt aacggtgcgg atgtcggaaa aaccatagac aatggtttgt atctgctgat
    21061 tatttatttt tcctggttcg cggtgttttt atcgctgtgg tacatgggga aagtgataaa
    21121 aatgatgatc aacgcttttg gtgataatcg caattttcgc gtgcaattat atcttttcac
    21181 tccggtatcg ctgtttttta ccggttcgat atttagcccg gaatatgcat ttttaatcgt
    21241 ctgtccgttt attttgcgaa aagcgttaaa tattacgagg taagaataag aacatgttgc
    21301 ttagcataat cactgtcgcg tttcgtaacc tcgaagggat agtcaaaaca catgcctcgc
    21361 tggcgcatct ggcgcaggcg gacgatatca gcttcgaatg gattgttgtc gatggcggtt
    21421 ccaatgacgg cacccgtgag tacctggaaa atctcaatgg tatctataac ctacgctttg
    21481 tcagcgaacc agataacggt atctacgacg ccatgaacaa gggcattgcg atggcacaag
    21541 gcaagttcgc gctgtttctc aactcgggcg atatttttca tcaggatgcc gcaaatttta
    21601 tccgtaagtt aaaagtgcaa aaagataacg tgatgatcac cggcgatgcg ctgctggatt
    21661 ttggcgacgg gcataaaatt aaacgtagcg ccaaaccggg ctggtatatt tatcacagcc
    21721 tacccgccag tcatcaggcg atatttttcc cggtatccgg cttgaagaaa tggcgttatg
    21781 acctggaata taaagtttct tccgattacg cgctggcggc caaaatgtat agagccggtt
    21841 atgcatttaa aaaactcaat ggcctggtgt ctgaattttc tatgggtggg gtatcaacca
    21901 ccaataatat ggaattgtgt gctgacgcca aaaaagtcca acggcaaata ttacatgtgc
    21961 ctggcttctg ggcagaatta tcctggcatt tacgccagcg tactacctca aagacgaaag
    22021 ccttatataa caaaagctga atataaggga aaacgatgca agatttaagc ggtttctcgg
    22081 taccgaaagg gttccggggc agcaatgcta ttaaagtgca attgtggtgg gcggtacagg
    22141 caacaatatt tgcctggtcg ccacaagtat tgtatcgctg gcgggctttt ttattacgtt
    22201 tattcggcgc aaaaatagga aaaaacgtag ttattcgtcc gtcagtaaaa attacctatc
    22261 cgtggaaatt aaccttaggt gattacgcgt gggtcggcga tgaggtcaat ttatataccc
    22321 tcggtgaaat aaccattggc gcacattcgg tgatatcgca gaaaagttat ttatgcaccg
    22381 ggagccacga ccatgcaagt caacatttca ccattaacgc cacgcctatt gtgattggcg
    22441 agaaatgctg gctggcaacc gatgtttttg ttgccccagg cgtcacgatc ggcaacggca
    22501 ccgtcgtggg tgcgcgaagc agtgttttta aatcgcttcc ggcaaatgtg gtttgccggg
    22561 ggaatcccgc agtggtgata cgcgaacgcg ttgaaactga ataaattcaa aaaatacaga
    22621 ggaataagac atgtcaaaag tcgctctcat caccggtgta accggacaag acggttctta
    22681 cctggcagag tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc gtcgcgcatc
    22741 gtcattcaac accgagcgcg tggatcacat ttatcaggat ccgcacacct gcaacccgaa
    22801 attccatctg cattatggcg acctgagtga tacctccaac ctgacgcgca ttttgcgtga
    22861 agtacagccg gatgaagtgt acaacctggg ggcaatgagc cacgttgcgg tttcttttga
    22921 gtcaccggaa tataccgcag acgtcgatgc gatgggtacg ctgcgcctgc tcgaggcgat
    22981 ccgcttcctc ggtctggaaa agaaaacccg tttttatcag gcttccacct ctgaactgta
    23041 tggtctggtg caggaaattc cgcagaaaga gaccacgccg ttctatccac gctctccgta
    23101 tgcggtcgcc aaactgtatg cctactggat caccgttaac taccgcgaat cctacggcat
    23161 gtatgcctgt aacggtattc tcttcaacca tgaatccccg cgccgcggcg aaaccttcgt
    23221 tacccgcaaa atcacccgcg caatcgccaa catcgcccag gggctggagt cgtgcctgta
    23281 cctcggcaat atggattccc tgcgtgactg gggccatgcc aaagactacg taaaaatgca
    23341 gtggatgatg ctgcaacagg aacagccgga agatttcgta attgctaccg gcgttcagta
    23401 ctccgtgcgt cagttcgtag aaatggcggc ggcacagttg ggtatcaaac tgcgctttga
    23461 aggcacgggc gttgaagaga agggcattgt ggtttccgtc accgggcatg atgcgccggg
    23521 cgttaaaccg ggcgatgtga ttatcgccgt tgacccgcgt tacttccgcc cggcagaagt
    23581 tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa ctgggctgga aaccggaaat
    23641 caccctcaga gagatggtgt ctgaaatggt ggctaatgac ctcgaagcgg cgaaaaaaca
    23701 ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat aaacatgcgt
    23761 aaacaacgaa tctttatcgc tggccatcgt gggatggttg gttccgccat caggcgtcag
    23821 ctcgaacagc gcggtgatgt ggaactggta ttacgcaccc gcgacgagct gaacctgctg
    23881 gacagccgcg ccgtgcatga tttctttgcc agcgaacgta ttgaccaggt ctatctggcg
    23941 gcggcgaaag tgggcggcat tgttgccaac aacacctatc cggcggattt catctaccag
    24001 aacatgatga ttgagagcaa tatcattcac gctgcgcatc agaacgacgt gaacaaactg
    24061 ctgtttctcg gatcgtcctg catctacccg aaactggcaa aacagccgat ggcagaaagc
    24121 gagttgttgc agggcacgct ggagccgacc aacgaacctt atgccattgc caaaatcgcc
    24181 gggatcaaac tgtgcgaatc ttacaaccgc cagtacggac gcgattaccg ctcggtcatg
    24241 ccgaccaacc tgtacgggcc gcacgacaac ttccatccga gtaactcgca tgtgatccca
    24301 gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg cgccggacgt ggtggtgtgg
    24361 ggcagcggta caccgatgcg tgaatttctg cacgtcgatg atatggcggc ggcgagcatt
    24421 catgtgatgg agctggcgca cgaagtctgg ctggaaacca cccagccgat gctgtcacac
    24481 attaacgtcg gcacgggcgt tgactgcact atccgcgagc tggcgcaaac catcgccaaa
    24541 gtggtgggtt acaaaggtcg ggtggttttt gatgccagta aaccggatgg tacgccgcgc
    24601 aaactgctgg atgtgacgcg cctgcatcag cttggctggt atcacgaaat ctcactggaa
    24661 gcggggcttg ccagcactta ccagtggttc cttgagaatc aagaccgctt tcgggggtaa
    24721 tgatgttttt acgtcaggaa gactttgcca cggtagtgcg ctccactccg cttgtctctc
    24781 tcgactttat tgtcgagaac agtcgcggcg agtttctgct tggcaaaaga accaaccgcc
    24841 cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca gaaagacgaa acgctggaag
    24901 ccgcatttga gcggctgacg atggcggaac tggggctgcg tctgccgata acagcaggcc
    24961 agttttacgg tgtctggcag cacttttatg acgataactt ctccggcacg gatttcacca
    25021 ctcactatgt ggtgctcggt tttcgcttca gagtagtgga agaagagctg ttactgccgg
    25081 atgagcagca tgacgattac cgctggctga cgccggacgc actgctcgcc agtgacaatg
    25141 ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg tgccggagta cccggattat
    25201 gaaaatactg gtctacggca ttaactactc gccggagtta accggcatcg gcaaatacac
    25261 cggcgagatg gtggaatggc tggcggcaca aggtcatgag gtgcgggtca ttaccgcacc
    25321 gccttactac ccgcaatggc aggtgggtga gaactattcc gcctggcgct acaaacgaga
    25381 agagggggcc gccacggtgt ggcgctgccc gctgtacgtg ccaaaacagc cgagcaccct
    25441 gaaacgcctg ttgcatctcg gcagttttgc cgtcagcagt tttttcccgt tgatggcgca
    25501 acgtcgctgg aagccggatc gcattatcgg cgtggtgcca acgctgtttt gcacgccggg
    25561 aatgcgcctg ctggcgaaac tctctggtgc gcgtaccgtg ctgcatattc aggattacga
    25621 agtagacgcc atgctggggc tgggccttgc cggaaaaggc aaaggcggca aagtggcaca
    25681 gctggcaacg gcgttcgaac gtagcggact gcataacgtc gataacgtct ccacgatttc
    25741 gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg gcggataacg tcatcttctt
    25801 ccccaactgg tcggaaattg cccgttttcg gcatgttgca gacgccgatg ttgatgccct
    25861 tcgtaaccag cttggcctgc cggataacaa aaaaatcatt ctttactccg gcaatattgg
    25921 tgaaaagcag gggctggaaa acgttattga agctgccgat cgtctgcgcg atgaaccgct
    25981 gatttttgcc attgtcgggc agggcggcgg caaagcgcgg ctggaaaaaa tggcgcagca
    26041 gcgtggactg cgcaacatgc aattcttccc gctgcaatcg tatgacgctt tacccgcact
    26101 gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc ggcgcggcag atgccgtatt
    26161 gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac gcggtgatta ccgcagaggc
    26221 ccacacagaa ctggggcagc tttgcgaaac ctttccgggc attgcggttt gcgtagaacc
    26281 ggaatcggtc gaggcgctgg tggcggggat ccgtcaggcg ctcctgctgc ccaaacacaa
    26341 cacagtggca cgtgaatatg ccgaacgcac actcgataaa gagaacgtgt tacgtcaatt
    26401 tataaatgat attcggggat aattatggcg cagtcgaaac tctatccaat tgtgatggca
    26461 ggtggctccg gtagccgctt atggccgctt tcccgcgtac tttaccccaa gcagttttta
    26521 tgcctgaaag gcgatctcac catgctgcaa accaccatct gccgcctgaa cggcgtggag
    26581 tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct ttattgtcgc ggaacagctg
    26641 cgtcaactga acaaactcac cgagaacatt attctcgaac cggcagggcg taacactgca
    26701 cctgccattg cgctggcggc gctggcggca aaacgtcata gcccggagag cgacccgtta
    26761 atgctggtgc tggcggcgga tcatgtgatt gccgatgaag acgcttttcg caccgccgtg
    26821 cgtaatgcca tgccatatgc cgaagcgggc aagctggtga ccttcggcat tgtgccggat
    26881 ctacctgaaa ccggttatgg ctatattcgt cgcggtgaag tgtctgctgg tgagcaggat
    26941 acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga atctggaaac cgcccaggcc
    27001 tatgtggcaa gcggcgaata ttactggaac agcggcatgt tcctgttccg cgccggacgc
    27061 tatctcgaag aactgaaaaa atatcgcccg gatatcctcg atgtctgcga aaaagcgatg
    27121 agcgccgtcg atccggatct cgattttatt cgcgtggatg aagaagcgtt tctcgcctgc
    27181 ccagaagagt cggtggatta cgcggtcatg gaacgtacgg cagatgccgt tgtggtgccg
    27241 atggatgcgg gctggagcga tgtcggttcc tggtcctctt tatgggagat cagtgcccac
    27301 accgccgagg gcaacgtttg ccacggcgat gtgattaatc acaaaactga aaacagctat
    27361 gtgtacgccg aatctggcct ggtcaccacc gtcggggtga aagatttggt ggtagtgcag
    27421 accaaagatg cagtgctgat tgccgaccgt aacgcggtgc aggatgtgaa aaaagtggtc
    27481 gagcagatca aagccgacgg tcgccatgag catcgggtac atcgcgaagt gtatcgtccg
    27541 tggggcaaat atgactctat cgacgcgggc gaccgctacc aggtgaaacg catcaccgtg
    27601 aaaccgggcg agggcttgtc ggtacagatg caccatcacc gcgcggaaca ctgggtggtt
    27661 gtcgcgggaa cggcaaaagt cactattgac ggtgatatca aactgcttgg tgaaaacgag
    27721 tccatttata ttccgctggg ggcgacgcac tgcctggaaa acccggggaa aattccgctc
    27781 gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt gcgcttcgcg
    27841 gatcgctacg gacgggtgta aacgtcgcat caggcgctgt ttgtcggatg cggcgtgaac
    27901 gccttatccg acctacggtt cggttttgta ggcctgataa gacgcggcag cgtcgcatca
    27961 ggcgctgttt gtcggatgcg gcgtgaacgc cttatccaac ctacggttcg gatttgtagg
    28021 cctgataaga cgcggcagcg tcgcatcagg caatgaatgc gaaaccgcga tgtaaataac
    28081 gacaaaaaca aaattggccg cttcggtcat ggccaactat tgcctgaaaa agggtaacga
    28141 tatgaaaaaa ttaacctgct ttaaagccta tgatattcgc ggaaaactag gcgaagaact
    28201 gaatgaagat atcgcctggc gcattggtcg cgcctatggc gaatttctca aaccgaaaac
    28261 cattgtgtta ggcggtgatg tccgcctcac cagcgaaacc ttaaaactgg cgctggcgaa
    28321 aggtttgcaa gatgcgggcg ttgatgtgct ggatattggc atgtccggca ccgaagagat
    28381 ctatttcgcc acgttccatc tcggcgtgga tggcggcatt gaagttaccg ccagccataa
    28441 tccgatggat tacaacggta tgaagctggt gcgcgaaggg gctcgcccaa tcagcggtga
    28501 taccggactg cgcgatgtcc agcgtctggc agaagccaat gacttccctc ctgtcgatga
    28561 aaccaaacgc ggtcgctatc agcaaatcaa cctgcgtgac gcttacgttg atcacctgtt
    28621 cggttatatc aacgtcaaaa acctcacgcc gctcaagctg gtgatcaact ccgggaacgg
    28681 cgcagcgggt ccggtggtgg acgccatcga agcccgcttt aaagccctcg gcgcacctgt
    28741 ggaattaatc aaagtgcaca acacgccgga cggcaatttc cccaacggta ttcctaaccc
    28801 gctgctgccg gaatgccgcg acgacacccg caatgcggtc atcaaacacg gcgcggatat
    28861 gggcattgcc tttgatggag attttgaccg ctgtttcctg tttgacgaaa aagggcagtt
    28921 tatcgagggc tattacattg tcggcctgct ggcagaagca ttcctcgaaa aaaatcccgg
    28981 cgcgaagatc atccacgatc cacgtctctc ctggaatacc gttgatgtgg tgactgccgc
    29041 aggcggcacc ccggtaatgt cgaaaaccgg acacgccttt attaaagaac gtatgcgcaa
    29101 ggaagacgcc atctacggtg gcgaaatgag cgcccaccac tatttccgtg atttcgctta
    29161 ctgcgacagc ggcatgatcc cgtggctgct ggtcgccgaa ctggtgtgcc tgaaagagaa
    29221 aacgctgggc gaactggtac gcgaccggat ggctgcgttt ccggcaagcg gtgagatcaa
    29281 cagcaaactg gcgcaacccg ttgaggcgat taaccgcgtg gaacagcatt ttagccgtga
    29341 ggcgctggcg gtggatcgca ccgatggcat cagcatgacc tttgccgact ggcgctttaa
    29401 cctgcgctcc tccaacaccg aaccggtggt gcggttgaat gtggaatcgc gcggcgatgt
    29461 gccgctgatg gaagcgcgaa cgcgaactct gctgacgttg ctgaacgagt aatgtcggat
    29521 cttcccttac cccactgcgg gtaaggggct aataacagga acaacgatga caaatctaaa
    29581 aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct atggtgcaac gcttttcaga
    29641 tatcaccatc atgtttgccg ggctatggct ggtttgcgaa gtgagcggac tgtcattcct
    29701 ctacatgcac ctgttggtgg cgctgattac gctggtggtg ttccagatgc tgggcggcat
    29761 caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg acagaatttg ccctgctgct
    29821 acaaaactgg accttaagcg tgattttcag cgccggactg gtggcgttca acaatgattt
    29881 cgacacgcaa ctgaaaatct ggctggcgtg gtatgggctg accagtatcg gactggtggt
    29941 ttgccgttca tgtattcgca ttggggcggg ctggctgcgt aatcatggct ataacaagcg
    30001 catggtcgcc gtagcggggg atttagccgc cggacaaatg ctgatggaga gcttccgtaa
    30061 tcagccgtgg ttagggtttg aagtggtggg cgtataccac gacccaaaac cgggcggcgt
    30121 ttctaacgac tgggcgggca atctgcaaca actggtcgag gatgcgaaag cgggcaagat
    30181 tcataacgtc tatatcgcga tgcagatgtg cgacggcgcg cgagtgaaaa aactggtcca
    30241 tcaactggcg gacaccacct gttcggtgct gctgatcccc gatgtcttta ccttcaacat
    30301 tctccattca cgcctcgaag agatgaatgg cgtaccggtg gtgccgctgt atgacacgcc
    30361 gctttccggg gttaaccgcc tgctcaaacg tgcggaagac attgtgctgg cgacgcttat
    30421 cctgctgctg atctccccgg tgctgtgctg cattgcgctg gcggtgaaac tgagctcacc
    30481 ggggccggtt attttccgcc agactcgcta cggcatggat ggcaaaccga tcaaagtgtg
    30541 gaagttccga tccatgaaag tgatggagaa cgacaaagtg gtgactcagg cgacgcagaa
    30601 cgatccgcgc gtcaccaaag tggggaactt tctgcgccgc acctcgctgg acgaattgcc
    30661 gcagtttatc aatgtgctga ctggcgggat gtcgattgtc ggtccacgtc cgcacgcggt
    30721 ggcgcataac gaacagtatc gacagctcat tgaaggctac atgctgcgcc ataaggtgaa
    30781 accgggcatt accggctggg cgcagattaa tggctggcgt ggcgaaaccg acacgctgga
    30841 gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc cgcgaatgga gcgtctggtt
    30901 cgatatcaaa atcgttttcc tgacggtatt caagggcttc gttaacaaag cggcatattg
    30961 atatgagctt acgtgaaaaa accatcagcg gcgcgaagtg gtcggcgatt gccacggtga
    31021 tcatcatcgg cctcgggctg gtgcagatga ccgtgctggc gcggattatc gacaaccacc
    31081 agttcggcct gcttaccgtg tcgctggtga ttatcgcgct ggcagatacg ctttctgact
    31141 tcggtatcgc taactcgatt attcagcgaa aagaaatcag tcacctggaa cttaccacgt
    31201 tgtactggct gaacgtcggg ctggggcttg tggtgtgcgt ggcggtgttt ttgttgagtg
    31261 atctcattgg tgacgtgctg aacaacccag acctggcacc gttgattaaa acattgtcgc
    31321 tggcgtttgt ggtgatcccc cacggacagc agttccgcgc gttgatgcaa aaagagctgg
    31381 agttcaacaa aatcggcatg atcgaaacca gcgcggtgct ggcgggcttc acttttacgg
    31441 tggttagcgc ccatttctgg ccactggcga tgaccgcgat cctcggttat ctggtcaata
    31501 gtgcggtaag aacgctgctg tttggctact ttggtcgcaa aatttatcgc cccggtctgc
    31561 atttctcgct ggcgtcggtg gcaccgaact tacgctttgg tgcctggctg acggcggaca
    31621 gcatcatcaa ctatctcaat accaaccttt caacgctggt gctggcgcgt attctcggcg
    31681 cgggcgtggc agggggatac aacctggcgt acaacgtggc cgttgtgccg ccgatgaagc
    31741 tgaacccaat catcacccgc gtgttgtttc cggcatttgc caaaattcag gacgacaccg
    31801 aaaagctgcg cgttaacttc tacaagctgc tgtcggtagt ggggattatc aactttccgg
    31861 cgctgctggg gctgatggtg gtgtcgaata actgtgtacc gctggtcttt ggtgagaagt
    31921 ggaacagcat tattccggtg ctgcaattgc tgtgtgtggt ggggctgctg cgctcagtgg
    31981 ggaacccgat tggttcgctg ctgatggcga aagcgcgggt cgatatcagc tttaaattca
    32041 acgtattcaa aacctttatg tttattccgg cgattgttat tggtgggcag atggcgggcg
    32101 caatcggcgt cacgcttggt ttcctgctgg tgcagattat caacaccatt ctgagctatt
    32161 tcgtgatgat taaaccggtg ctcggttcca gttatcgtca gtacatcctg agtttgtggc
    32221 tgccgtttta tctctcgctg ccgacactgg tggtcagtta tgcgctgggc ctattgctga
    32281 aagggcaact ggcgctgggg atgctgctgg cggtgcaaat agccgcgggc gtgctggcgt
    32341 ttgtggtgat gattgtgctg tcgcgccatc cgctggtggt ggaagtgaag cgtcagtttt
    32401 gtcgcagcga aaaaatgaaa atgcttttac gggcggggtg aatggctatc cccgtaaggt
    32461 cgtgcgcatt ttccccctca ccctaaccct ctcccctggg ggcgagggga ctgatcgagc
    32521 acagctttga atatgtcacc ggtttaactc ttaatcatca accatagcac cgacgcaatt
    32581 tagaactcct tttgaggtca taatgaaatt acttattctg ggcaaccaca cttgcggcaa
    32641 tcgtggcgac agcgccatcc tgcgcggctt acttgatgcc atcaacatcc tcaatccaca
    32701 cgccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc tcaaccgccc
    32761 ggtaatgggc gatccgctgt tcctgcaaat gaaaaaacac aacagcgcgg cgggcgttgt
    32821 cgggcgcgtt aaaaaagtcc tccgtcgccg ctatcagcat caggtattgc tctcacgcgt
    32881 caccgacact ggcaagctgc gtaatatcgc catcgcccag ggattcactg acttcgtgcg
    32941 cctgctgtca ggttacgacg ccattattca ggtcggcgga tcgttttttg tcgatctcta
    33001 cggcgtgccg cagtttgaac acgcgctttg cactttcatg gcgaaaaagc cgctatttat
    33061 gattggtcac agcgttggtc cattccagga tgagcaattt aaccaactgg cgaactacgt
    33121 ttttggtcac tgcgacgcgc tgatcctgcg cgaatcggtc agccttgaac tgatgaaacg
    33181 cagcaatatc accaccgcaa aagtcgaaca tggcgtcgat actgcgtggc tggtcgatca
    33241 ccacacagaa gacttcaccg ccagctatgc cgttcaacac tggctggacg ttgccgcaca
    33301 acagaaaacg gtagcaatta ccctgcgcga actggcaccg ttcgacaaac gtctcggcac
    33361 cactcagcaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc tcgacgaagg
    33421 gtatcaggtg attgcgcttt ccacctgtac gggcatcgac agctataaca aagacgaccg
    33481 catggtggcg ctcaatcttc gccagcacat cagcgatcct gcccgttacc acgtggtgat
    33541 ggatgaactc aacgatctgg aaatgggcaa aattctgggg gcctgtgaac tcaccgtcgg
    33601 tacgcgcctg cactctgcca ttatctcgat gaactttgcc actccggcga ttgccatcaa
    33661 ctatgaacat aaatccgccg ggattatgca acaactgggg ctaccagaga tggcaattga
    33721 tatccgtcat ttattagacg gcagcctgca agcgatggtt gcggatacct taggccagct
    33781 tccggcactg aacacgcgac ttaacgaagc cgtcagtcgc gaacgtcaga cgggaatgca
    33841 gatggtgaaa tctgtgcttg aacgcatcgg ggaggcgaaa tgaaggtcgg cttcttttta
    33901 ctgaaatttc cactgtcgtc ggaaaccttc gtcctcaatc aaattaccgc gtttattgat
    33961 atgggctttg aggtggagat tgtcgcgctg caaaaaggcg acacacaaaa cacccacgcg
    34021 gcatggacaa aatataacct tgccgccaga acccgctggt tacaggacga gccacaaggc
    34081 aaagtggcga aactgcgcca ccgcgccagc cagacgttgc gcggtattca tcgtaaaaat
    34141 acctggcagg cgcttaacct caaacgctat ggtgctgagt cgcggaacct gattttgtct
    34201 gccatttgcg gccaggtcgc aacaccgttt catgccgatg tgtttatcgc tcattttggc
    34261 cctgctgggg taaccgcggc aaaactacgc gaactgggtg tcattcgcgg caaaattgcc
    34321 actatcttcc acggtattga tatctccagt cgggaagtgc tcaaccacta cactcccgaa
    34381 tatcaacaac tgtttcgccg tggcgacctg atgttaccaa taagcaatct gtgggccgga
    34441 aggctgcaaa aaatgggctg cccgagggaa aaaatcgccg tatcgcgcat gggcgtggac
    34501 atgacgcgtt ttagcccgcg tccggtgaaa gcgcccgcaa cgccgctgga aatcatctcc
    34561 gtcgcacgct taaccgagaa aaaaggcctg catgtggcga ttgaagcctg ccgccagttg
    34621 aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg ggaacgacgc
    34681 ctgcgcaccc tcatcgaaca atatcaactg gaagatgtga tagagatgcc gggctttaaa
    34741 ccgagccatg aagtgaaagc gatgctcgat gacgcggatg tcttcctgtt gccatcggtt
    34801 acaggtgcgg atggcgatat ggaaggtatt ccggtggcgc taatggaagc gatggcggtt
    34861 ggcattccgg ttgtttctac tctgcacagc ggaataccgg aactggtgga ggccgataaa
    34921 tccggttggc tggtgcctga gaacgatgct cgcgcactgg cgcaacgact ggcggcgttt
    34981 agccaactgg acaccgacga acttactacg gttgtcaaac gtgcgcgcga aaaagtcgaa
    35041 cacgatttta accagcaggt gatcaatcga gaactcgcca gcttgctgca ggctttatag
    35101 aggagaacgt atgccattta aaaaactctc ccgacgcacc ttcctgacgg caagctcggc
    35161 gcttgccttc ctccataccc ctttcgctcg cgcgcttccc gcccgacaaa tcgttaacat
    35221 taacgactac aacccacacg actggatcgc ctcatttaaa caagccttca gcgaagggca
    35281 aacggtcgtc gtgcctgccg gattcgtttg tgaaaatatc aacaccggca tcttcattcc
    35341 tcctggcaaa acgttgcaca tccttggaag cctgcgcggc aacggcagag ggcgatttgt
    35401 cttacaggac ggcagccagg tgacagggga gaagggcggc cgtatgcata acatcaccct
    35461 ggatgtgcgc ggctctgact gcatcatcaa agggttggcg atgagcggtt ttggcccggt
    35521 aacgcagatt tatatcggcg gcaaaaataa acgcgtcatg cgcaacttga ccatcgataa
    35581 cctgaccgtc agccacgcta attacgccat tttacgccag ggatttcata accagattat
    35641 cggtgccaac atcaccaatt gtaagttcag cgacttacag ggcgacgcca ttgaatggaa
    35701 tgtagcaatc aatgatcgcg acattttgat atccgaccat gtcatcgagc gcatcaactg
    35761 tactaacggc aaaatcaact ggggtattgg cataggactg gcaggaagta cttacgataa
    35821 taattatccg gaaaaccagg cagtgaaaaa ctttgtcgtt gcgaatatca cgggatcaga
    35881 ttgtcggcaa ctgatccatg ttgaaaatgg taaacatttt gttatccgta atatcaaagc
    35941 tcgtaatatc acgccggatt tcagtaagaa agcaggtatt gataacgcca cagttgctat
    36001 ttatggttgt gacaactttg tgattgataa tattgaaatg attaatagtg ccgggatgtt
    36061 aatcggctat ggggtaatta aaggcaaata tctctcgata ccgcagaatt tccgagtgaa
    36121 tgatattcaa ctggataata ctcatcttgc ttataaattg cgcggcatcc aaatctccgc
    36181 cgggaatgct gtctcctttg tggcgctgac taacattgag atgaagcgtg cgtcgctgga
    36241 gttacacaac aaaccgcaac atctttttat gcgaaatatc aatgtgatgc aggaatcctc
    36301 agttggaccc gcattgagca tgaactttga catgcgcaaa gacgttcgcg gcgtgtttat
    36361 ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcggtgaatg aaaaaggaca
    36421 aatctccgtc gatatcgaca ggattaatca ccatattgtt aatgtggaga agattaactt
    36481 tagattgcca gaacggaggg agtagatttg cgaccattcc tggaaaaatg gagccatact
    36541 taggaacaat gctactgcaa tccacaacga agcggcgtaa catcacaagt aattcagtaa
    36601 tcaattcagg gtaattgatg ctggcgaaaa aaatcgaaca agctataatt cagcaaccat
    36661 tttacaggtg gatgaaataa tgacgaattt aaaagcagtt attcctgtag cgggtcttgg
    36721 gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa
    36781 gccaatgatt cagtacatcg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
    36841 ggtaactcac gcgtccaaga acgcggtcga aaaccacttc gacacctctt atgaattaga
    36901 atctctcctt gaacagcgcg tgaagcgtca actactggcg gaagtgcagt ccatctgtcc
    36961 gccgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttag gccactccat
    37021 tttgtgtgca cgacccgcca ttggtgacaa tccatttgtc gtggtactgc cggacgttgt
    37081 gatcgacgac gccagtgccg acccgctgcg ctacaacctt gctgccatga ttgcgcgttt
    37141 caatgaaacg ggccgtagcc aggtgctggc aaaacgtatg ccgggtgacc tctctgaata
    37201 ctcggtcatc cagaccaaag aaccactgga ccgtgaaggt aaagtcagcc gcattgttga
    37261 attcatcgaa aaaccggatc agccgcagac gctggactca gacatcatgg ccgttggtcg
    37321 ctatgtgctt tctgcagata tttggccgga acttgaacgc actcagcctg gtgcatgggg
    37381 gcgtattcag ctgactgatg ccatcgctga actggcgaaa aaacagtccg ttgatgccat
    37441 gctgatgaca ggtgacagct acgactgcgg taaaaaaatg gggtatatgc aggcatttgt
    37501 gaagtatgga ctacgcaacc tgaaagaagg ggcgaagttc cgtaaaggga ttgagaagct
    37561 gttaagcgaa taatgaaaat ctgaccgaat gtaacggttg ataagaaaat tataacggca
    37621 gtgaagattc gtggcgaaag taatttgttg cgaattttcc tgccgttgtt ttatataaac
    37681 aatcagaata acaacgagtt agcaatagga ttttagtcaa agttttccag gattttcctt
    37741 gtttccagag cggattggta agacaattag cgtttgaatt tttcgggttt agcgcgagtg
    37801 ggtaacgctc gctacatcgt aggtatgcat gcagtgctct ggtagctgta aagccagggg
    37861 cggtagcgtg tctaggggca gggaaagatt atcagaagta cactttttgt actaaataat
    37921 tcgcatttta tgttcaaaaa ttgagatatc cctcattacc taaaactgtt tttcacagct
    37981 tatatatgat caaatactcc ttgcttaaat aaggagaaca aaatggaact taaaaaattg
    38041 atggaacata tttctgttat tcccgattac agacaagcct ggcagggaaa gatcacgtaa
    38101 tgctgctttt ttgtactaaa caattcgca
//
LOCUS       DAYPHN010000029        37306 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000029 DAYPHN010000000
VERSION     DAYPHN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.029"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            147..3860
                     /gene="yfaL"
                     /locus_tag="ACG0BW_001433"
     CDS             147..3860
                     /gene="yfaL"
                     /locus_tag="ACG0BW_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFK7258417.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVIDSCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTNVIFSDNVAGGYGGAIYSSGTNDTGVVDLSVTNA
                     VFRNNIANDGKGGGIYTINNDVYLSKVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDIT
                     HPSGFTIINNTAFTNNTAEGYGGAIYTNSVSAPYLIDISIDDSYSQNGGVLIDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGVITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLTLEGDAADLAALQDDPQSIVLNGGVLDLSDFATWQS
                     GTSYNDGLAVSGSGGTVIGSQDVVDLAGGDNLHIGGDGLVSLANNNSYLGTTQIASGT
                     LVVSDNSQLGDTHYNRQVIFTDNQQESVMEITANVDTRSTTTEHGRDIEMRADGEVAV
                     DAGVDTQWGGLMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTLKGDVA
                     DIFPYASSLWVGDGATFKTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNAS
                     LFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQNGVAGD
                     TLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVVINPITGIGEPTSTGI
                     KVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPDPD
                     PTPDPDPTPDPDPTPDPDPTPAYQPVLNAKVGGYFNNLRAANQAFVMERHDHAGGNGQ
                     TLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGAVGGYSDNQG
                     DSRSNMTGTCADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGA
                     DHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDI
                     QTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKQRGEIKVGVTGN
                     ISQRVSLRGNVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3921..4643)
                     /gene="ubiG"
                     /locus_tag="ACG0BW_001434"
     CDS             complement(3921..4643)
                     /gene="ubiG"
                     /locus_tag="ACG0BW_001434"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFK7258418.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4790..7417
                     /gene="gyrA"
                     /locus_tag="ACG0BW_001435"
     CDS             4790..7417
                     /gene="gyrA"
                     /locus_tag="ACG0BW_001435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFK7258419.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7566..9254
                     /gene="yfaA"
                     /locus_tag="ACG0BW_001436"
     CDS             7566..9254
                     /gene="yfaA"
                     /locus_tag="ACG0BW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFK7258420.1"
                     /translation="MSGEKKAKGWRFYGLVCLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9251..9874
                     /gene="yfaT"
                     /locus_tag="ACG0BW_001437"
     CDS             9251..9874
                     /gene="yfaT"
                     /locus_tag="ACG0BW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFK7258421.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFSANEALKIHDSKWLKSNGIASQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9898..14412
                     /locus_tag="ACG0BW_001438"
     CDS             9898..14412
                     /locus_tag="ACG0BW_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFK7258422.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTINVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRSQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFSKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNTGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLAGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMMLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISINKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQRVAA
                     GSEWTGMQVK"
     gene            14413..16062
                     /gene="yfaQ"
                     /locus_tag="ACG0BW_001439"
     CDS             14413..16062
                     /gene="yfaQ"
                     /locus_tag="ACG0BW_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFK7258423.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPEASVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16067..16843
                     /gene="yfaP"
                     /locus_tag="ACG0BW_001440"
     CDS             16067..16843
                     /gene="yfaP"
                     /locus_tag="ACG0BW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFK7258424.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAVGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGVIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16917..18101)
                     /gene="atoB"
                     /locus_tag="ACG0BW_001441"
     CDS             complement(16917..18101)
                     /gene="atoB"
                     /locus_tag="ACG0BW_001441"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFK7258425.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKTLQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18132..19454)
                     /gene="atoE"
                     /locus_tag="ACG0BW_001442"
     CDS             complement(18132..19454)
                     /gene="atoE"
                     /locus_tag="ACG0BW_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFK7258426.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVVGLTLF"
     gene            complement(19451..20101)
                     /gene="atoA"
                     /locus_tag="ACG0BW_001443"
     CDS             complement(19451..20101)
                     /gene="atoA"
                     /locus_tag="ACG0BW_001443"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFK7258427.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVCAKTEARFEVAADLNTQRGDL"
     gene            complement(20101..20763)
                     /gene="atoD"
                     /locus_tag="ACG0BW_001444"
     CDS             complement(20101..20763)
                     /gene="atoD"
                     /locus_tag="ACG0BW_001444"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFK7258428.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKIWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(20959..22344)
                     /gene="atoC"
                     /locus_tag="ACG0BW_001445"
     CDS             complement(20959..22344)
                     /gene="atoC"
                     /locus_tag="ACG0BW_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFK7258429.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTSPVGERNLKEEIKRVEKCIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22341..24167)
                     /gene="atoS"
                     /locus_tag="ACG0BW_001446"
     CDS             complement(22341..24167)
                     /gene="atoS"
                     /locus_tag="ACG0BW_001446"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFK7258430.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELNPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24334..27183
                     /gene="rcsC"
                     /locus_tag="ACG0BW_001447"
     CDS             24334..27183
                     /gene="rcsC"
                     /locus_tag="ACG0BW_001447"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFK7258431.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLENSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27383..28033)
                     /gene="rcsB"
                     /locus_tag="ACG0BW_001448"
     CDS             complement(27383..28033)
                     /gene="rcsB"
                     /locus_tag="ACG0BW_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFK7258432.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28050..30722)
                     /gene="rcsD"
                     /locus_tag="ACG0BW_001449"
     CDS             complement(28050..30722)
                     /gene="rcsD"
                     /locus_tag="ACG0BW_001449"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFK7258433.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31461..32564
                     /gene="ompC"
                     /locus_tag="ACG0BW_001450"
     CDS             31461..32564
                     /gene="ompC"
                     /locus_tag="ACG0BW_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFK7258434.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            32676..33731
                     /gene="apbE"
                     /locus_tag="ACG0BW_001451"
     CDS             32676..33731
                     /gene="apbE"
                     /locus_tag="ACG0BW_001451"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFK7258435.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALVADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            33805..34869
                     /gene="ada"
                     /locus_tag="ACG0BW_001452"
     CDS             33805..34869
                     /gene="ada"
                     /locus_tag="ACG0BW_001452"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFK7258436.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALTAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     ETLADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVLRKAQLLRREAENEER"
     gene            34869..35519
                     /gene="alkB"
                     /locus_tag="ACG0BW_001453"
     CDS             34869..35519
                     /gene="alkB"
                     /locus_tag="ACG0BW_001453"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFK7258437.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFTFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            35595..37238
                     /gene="yojI"
                     /locus_tag="ACG0BW_001454"
     CDS             35595..37238
                     /gene="yojI"
                     /locus_tag="ACG0BW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFK7258438.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLIEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9083 a         9200 c        10013 g         9010 t
ORIGIN      
        1 cttgaattat gacgcactga tattattcat gaaataataa caaaatagcc attgcaccgg
       61 gttgaacaat ttacaaaaga aaaataaccc atatcgtata atgcgattaa atatgccgtc
      121 ttatagaaaa tgatggtcag attaatatgc ggattatttt tctacgcaag gagtatttat
      181 ctttactccc gtcaatgatt gcatctcttt tctctgctaa cggtgtcgcg gcggtcattg
      241 attcatgtca gggatatgat atcaaagcga gttgtcacgc cagcaggcaa agcctttcag
      301 gcattacgca ggactggagc atcgccgatg ggcaatggct ggttttttcg gatatgacca
      361 ataacgccag cggtggggcc gtatttttgc aacaaggagc ggagttttct ctatcgccag
      421 aaaatgaaac tggaatgact ctgtttgcca ataatactgt ctcaggagaa tataataacg
      481 gcggggcaat atttgctaaa gaaaactcaa cgctgaatct tactaatgtt attttttccg
      541 ataacgttgc aggtggctat ggtggcgcaa tctattcttc tggtactaac gataccggtg
      601 tcgtcgattt aagtgtcact aacgccgtgt ttcgcaataa catcgctaat gacggcaaag
      661 gtggtggaat ttataccatc aacaatgacg tctatttaag taaagttatt tttgataaca
      721 accaggcata tacatcaaca agttacagtg atggcgatgg gggggcaatc gatgtcaccg
      781 ataataatag cgatatcacg catccgtcag gtttcacaat aataaataac accgcattta
      841 caaataacac tgccgaaggt tatggtgggg caatttatac caatagcgtg tcggctccct
      901 atcttattga tatttcgatt gatgacagct acagccagaa cggcggcgtg ttaatcgatg
      961 agaacaatag cgcggcaggt tatggggatg gtccttccac tgcggcgggc ggctttatgt
     1021 atctcggctt aagtgaagtt acctttgata ttgccgacgg aaaaacgctg gttatcggca
     1081 atacagagaa tgacggagct gttgactcta ttgctggtac cggggtaatc accaaaacag
     1141 gttccggcga tctggtactt aatgcagata acaatgactt tactggtgag atgcagatag
     1201 aaaacggtga agttaccctg ggccgcagca actccctgat gaatgtcggc gatacgcatt
     1261 gccaggacga tccgcaagac tgctatggtc tgacgatagg gagtattgat cagtatcaga
     1321 atcaggctga gctaaacgtt ggctcgaccc aacaaacttt tgtgcacgca ttgacgggct
     1381 ttcagaatgg cactttaaat atcgatgctg gtggcaacgt tactgttaat cagggcagtt
     1441 ttgctggcac catcgaaggt gctggtcagc tcaccattgc gcaaaacggc agctacgtgc
     1501 tggcaggggc tcagtcgatg gcgctaactg gcgatatagt cgttgatgat ggcgcggtgc
     1561 ttacgctgga aggcgacgcg gcagatcttg ccgctctcca ggacgatccg cagtcgatcg
     1621 tgttaaacgg cggtgtgctt gatctctctg atttcgccac ctggcagagc ggcacatcat
     1681 acaacgatgg ccttgcagtc agcggcagcg gcggtacggt tatcggcagt caggatgtgg
     1741 tggatctcgc agggggcgac aatttgcata tcggcggcga cgggctggtt agcctggcaa
     1801 acaataatag ttacctgggc accacgcaaa tcgcttccgg tacgctggtg gtgagcgaca
     1861 actcgcagct tggagatacc cattacaacc gccaggttat ctttaccgat aatcaacaag
     1921 aaagcgtgat ggagattact gccaatgtcg atactcgctc tacaacgact gagcatgggc
     1981 gtgatattga aatgcgcgcc gacggtgaag tggcagttga tgcgggggta gacacgcagt
     2041 ggggcggact gatggctgac agcagcgggc agcatcaaga tgagggtagc acattgacta
     2101 aaacgggggc gggtacgctg gaactgaccg ccagcggtac gacgcagtcg gcggtacgtg
     2161 tcgaagaagg caccctgaaa ggcgatgttg cggatatctt cccttatgct tcgtcgctgt
     2221 gggtcggtga cggggcaaca ttcaagactg gcgcggatca ggatattcag tcaattgatg
     2281 ctacttccag cggcactatc gacatcagcg atggtacggt tttgcgcctg accgggcagg
     2341 atacttccgt cgcccttaat gcctcactgt ttaacggcga tgggacgctg gtgaatgcca
     2401 ccgatggtgt gacgttgaca ggtgagctta ataccaacct tgaaactgac agcctgactt
     2461 atctttccaa cgtgacggtt aatggcaatc tgaccaatac gtccggtgcg gttagcctgc
     2521 aaaatggcgt cgctggcgat acgctgacgg taaacggtga ttataccggc ggcggtacgc
     2581 tactgctcga tagcgaatta aacggcgatg actcggcaag tgaccaactg gtgttgaacg
     2641 gtaatactgc tggcaacacg accgtggtaa ttaatcccat tacgggtatt ggtgagccga
     2701 catctacagg cattaaagtg gttgatttcg cagctgatcc cacgcagttt caaaacaatg
     2761 cgcagttcag tctggcaggt agtggctacg tcaatatggg agcgtatgac tacacgctgg
     2821 tggaagataa caacgactgg tatctgcgat cgcaagaagt aacgccgcca tcgccacctg
     2881 atcccgaccc gactcccgat cctgatccca caccggatcc cgatccaaca cccgacccag
     2941 accctacgcc tgcttaccag ccggtgctga atgccaaagt tggtggatat tttaataacc
     3001 tgcgggcggc aaatcaggcg tttgtgatgg agcgacacga tcacgctggt ggcaatggtc
     3061 agacgctgaa tttacgtgtt atcggcggag attatcatta cacagcagcg gggcaactgg
     3121 ctcagcatga agacacttct acggtgcagc ttagcggcga cttgtttagc gggcgctggg
     3181 gcacggatgg cgagtggatg cttggggcgg ttggtggcta cagtgataac cagggcgaca
     3241 gccgctcgaa tatgaccgga acttgcgccg ataaccagaa ccacggttat gccgttggtc
     3301 tgacctcaag ctggtttcag cacggtaatc agaagcaggg ggcctggctg gatagctggc
     3361 tgcaatacgc gtggtttaac aatgatgttt ccgaacatga agatggcgcg gatcattacc
     3421 actcatcggg gattatcgcc tcgctggaag ccgggtatca gtggttaccg gggcgtggtg
     3481 tggtgattga accgcaggcg caggtgattt atcagggcgt gcagcaggat gattttaccg
     3541 ccgctaacca tgcgcgcgtg tcacaatcgc agggggatga tattcagacg cggctgggtt
     3601 tacacagcga atggcgtacc gcggttggtg tcacaccaac attagatctg aattattatc
     3661 acgatcccca tgcgacggaa attgaagaag atggcagcac tatcagtgac gatgcggtga
     3721 agcaacgggg tgaaataaaa gtgggagtca cgggcaatat cagtcagcga gtgtccctgc
     3781 gtggtaacgt ggcgtggcag aaagggagtg atgattttgc ccagacggca gggtttttgt
     3841 cgatgacggt gaaatggtag ctgctttgcg cccggcattg tagccggatg tggtgcgagc
     3901 accttatccg gctaacaatt ttatttattc tgcgtgtgca gcatatagtt cacatcgacg
     3961 ccggggccga gtttaaaagt attagtgatc gggttgtaat gtaacccggt tatatgtcgc
     4021 tctttaaggc ttgtctgatc tacccagcca agcagttctg ccggttttat aaatttcttc
     4081 acgtcgtgcg tgcctttggg caccatgcgc aaaatatatt ccgcgccaac caccgccatc
     4141 agccatgact tgccgttgcg gttaagcgtc gagaaaaaga catcgccgcc cggtttcacc
     4201 agttgcgcac aggctctgac cactgactgc ggatcgggca cgtgctccag catctccatg
     4261 caggtcacca catcatactg cccggcatgt tttgccgcgt gctcttccac ggtttcctgc
     4321 acgtaatcca cctgaatgcc gctttccagc gcgtgcagct ttgccacctg caatggctca
     4381 aagcccatat ccagaccggt caccgtcgcg ccttcgcgcg ccatactctc ggccagaatg
     4441 ccgccgccac aaccgacatc gagtaccttt ttgccaaata aaccgccagc acgctcggca
     4501 atatagccca gacgcagcgg gttaatgcgg tgcagcggtt tgaactcacc ttcaagatcc
     4561 caccagcggg aggcgacagc ttcaaattta gcgatctctt cgtggtctac gttatggttt
     4621 accggcgatt tttcggcatt cattggcact tctactccgt aattggcaag acaaacgagt
     4681 atatcaggca ttggaggtga ataaagcgta taggtttacc tcaatctgcg cggctgtgtt
     4741 ataatttgcg acctttgaat ccgggataca gtagagggat agcggttaga tgagcgacct
     4801 tgcgagagaa attacaccgg tcaacattga ggaagagctg aagagctcct atctggatta
     4861 tgcgatgtcg gtcattgttg gccgtgcgct gccggatgtc cgagatggcc tgaagccggt
     4921 acaccgtcgc gtactttacg ccatgaacgt actaggcaat gactggaaca aagcctataa
     4981 aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac catccccatg gtgacttggc
     5041 ggtttatgac acgatcgtcc gtatggcgca gccattctcg ctgcgttaca tgctggtaga
     5101 cggtcagggt aacttcggtt ccatcgacgg cgactctgcg gcggcaatgc gttatacgga
     5161 aatccgtctg gcgaaaattg cccatgaact gatggccgat ctcgaaaaag agacggtcga
     5221 tttcgttgat aactatgacg gcacggaaaa aattcccgac gtcatgccaa ccaaaattcc
     5281 taacctgctg gtgaacggtt cttccggtat cgccgtaggt atggcaacca acatcccgcc
     5341 gcacaacctg acggaagtca tcaacggttg tctggcgtat atcgatgatg aagacatcag
     5401 cattgaaggg ctgatggaac acatcccggg gccggacttc ccgacggcgg caatcattaa
     5461 cggtcgtcgc ggtattgaag aagcttaccg taccggtcgt ggcaaggtat atatccgcgc
     5521 ccgtgccgaa gtggaagttg acgcaaaaac cggacgtgaa accattatcg tccacgaaat
     5581 tccgtatcag gtaaacaaag cgcgcctgat cgagaagatt gcggaactgg taaaagaaaa
     5641 acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac aaagacggta tgcgcatcgt
     5701 gattgaagtg aaacgcgacg cggtcggtga agttgtgctt aacaacctct actcccagac
     5761 ccagttgcag gtttctttcg gtatcaacat ggtggcattg caccatggtc agccgaagat
     5821 catgaacctg aaagacatca tcgcggcgtt tgttcgtcac cgccgtgaag tggtgacccg
     5881 tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct catatccttg aagcattagc
     5941 cgtggcgctg gcgaacatcg acccgatcat cgaactgatc cgtcatgcgc cgacgcctgc
     6001 agaagcgaaa actgcgctgg ttgctaatcc gtggcagctg ggcaacgttg ccgccatgct
     6061 ggaacgtgcg ggcgacgatg ctgcgcgtcc ggaatggctg gagccagagt tcggtgtgcg
     6121 tgatggtctg tactacctga ccgaacagca agctcaggcg attctggatc tgcgtttgca
     6181 gaaactgacc ggccttgagc atgaaaaact gctcgacgaa tacaaagagc tgctggatca
     6241 gatcgcagaa ctgctgcgta ttcttggcag tgccgatcgt ctgatggaag tgatccgcga
     6301 agagctggaa ctggttcgtg aacagttcgg tgacaaacgt cgcactgaaa tcaccgccaa
     6361 cagcgccgac atcaacctgg aagatctgat cactcaggaa gatgtggtcg tgacgctctc
     6421 tcaccagggc tacgtgaagt atcagccgct ttctgaatac gaagcgcagc gtcgtggcgg
     6481 gaaaggtaaa tctgccgcac gtattaaaga agaagacttt atcgaccgac tgctggtggc
     6541 gaacacccac gaccatattc tgtgcttctc cagccgtggt cgcgtctatt cgatgaaagt
     6601 ctatcagttg ccggaagcca ctcgtggcgc gcgcggtcgt ccgatcgtca acctgctgcc
     6661 gctggagcag gacgaacgaa tcactgccat cctgccggtg accgagtttg aagagggcgt
     6721 gaaagtcttc atggcgaccg ctaacggtac cgtgaagaaa actgtcctca ccgagttcaa
     6781 ccgtctgcgt accgccggta aagtggcgat caaacttgtt gaaggcgatg agctgatcgg
     6841 cgttgacctg accagcggcg aagacgaagt aatgctgttc tccgccgaag gtaaagtggt
     6901 gcgctttaaa gagtcttctg tccgtgcgat gggctgcaac accaccggtg ttcgcggtat
     6961 tcgtttaggc gaaggcgata aagtcgtctc tctgatcgtg cctcgtggcg atggcgcaat
     7021 cctcaccgca acgcagaacg gttatggtaa acgtaccgca gtggcggaat acccgactaa
     7081 gtcgcgtgcg acgaaagggg ttatctccat caaggttacc gaacgtaacg gtttagtggt
     7141 tggcgcggtg caggtagatg actgcgacca gatcatgatg atcaccgatg ccggtacgct
     7201 ggtacgtact cgcgtttcgg aaatcagcat cgtgggccgt aacacccagg gcgtgatcct
     7261 catccgtact gcggaagatg aaaacgtagt gggtctgcaa cgtgttgctg aaccggttga
     7321 cgaggaagat ctggatacca tcgacggcag tgccgcggaa ggggacgatg aaatcgctcc
     7381 ggaagtggac gttgacgacg agccagaaga agaataattt tacttcttca tgccaaaagg
     7441 gagctatctc ccttgtttga attgaaaagt ccaggctgca aagtctgggc ttttgtcgta
     7501 ttagggcgcg gtaaagtttt gctgtgctcg taaaaaatgg ctggctttac acaaggaatg
     7561 tggcaatgag tggtgaaaaa aaggcgaaag gctggcggtt ctatggtctt gtatgtttgg
     7621 gcgcaatagt actgctttcc gctggcgtct gggcgttgca atatgctggc agtgggccag
     7681 aaaaaacgtt gtcgccgctg gtggtgcaca acaatctgca aatcgatctc aatgagccgg
     7741 acctctttct cgacagcgac tctctgagcc agcttcccaa agacctcctt accattccgt
     7801 ttctccacga tgttctgagt gaagatttcg ttttctatta tcagaatcat gccgatcgtc
     7861 tgggcattga aggcagcatt cgccgcattg tctatgaaca cgatctcacg ctgaaagata
     7921 agctcttttc gtcactctta gatcagcccg cgcaggcggc gctatggcac gataaacaag
     7981 gccatctttc acattacatg gtgctgatcc agcgcagtgg tttaagcaaa ctgctggagc
     8041 cattgttgtt tgccgctacc agcgacagcc agttaagcaa aacggaaatc agtagtatta
     8101 agctaaatag tgaaaccatc ccggtttatc agttgcgcta taacggcaat aacgccctga
     8161 tgttcgcgac ttatcaggac aagatgctgg tgttttccag tacggatatg ctgtttaaag
     8221 atgaccagca ggataccgaa gccacggcga tcgcaagtga tttgttgagc ggtaaaaaac
     8281 gctggcaagc cagctttggc ctggaagagc gtgctgctga aaaaacgcca gtacgccagc
     8341 gcatcgtggt cagcgccagg ttgctggggt ttggctacca gcgattaatg ccttcttttg
     8401 ctggtgtacg cttcgaaatg agcaacgacg gctggcatag ctttgtggcg ttaaatgatg
     8461 aatccgcaag cgtagatgcc agtttcgatt ttacgccggt atggaacagt atgcctgccg
     8521 gggccagctt ctgtgtggcg gtgccgtatt cgcatggtat tgccgaagag atgctttcgc
     8581 acatcagcca ggaaaacgac aagctgaacg gggcgttaga tggtgcagcc gggctgtgct
     8641 ggtatgaaga ctcaaaatta caaaccccgc tatttgttgg tcaatttgat ggctcagccg
     8701 aacaggcaca actgcctggg aaactgttca cgcaaaatat tggtgcgcac gaaagcaaag
     8761 cgccagaagg tgttttgccg gtaagccaga ctcagcaggg cgaagcgcaa atctggcgtc
     8821 gcgaagtgag ttcccgatac ggccagtatc cgaaagcgca ggcggcgcaa cccgatcaat
     8881 taatgtcgga ttattttttc cgcgtgtcgc tggcgatgca aaacaaaacg ctgcttttct
     8941 ccctcgatga cacgctggtt aataacgccc tgcaaacact gaataaaacc cgcccggcaa
     9001 tggtggatgt aatacccact gatggcatcg ttccgctcta tatcaatcca caaggcgtgg
     9061 cgaaactgct gcgtaacgaa acgttgacca gtctgccgaa gaatctcgaa ccggtttttt
     9121 ataacgccgc acaaacttta ttaatgccga agctggacgc tttatctcaa caaccgcgtt
     9181 atgtcatgaa gctggcccag atggaacccg gtgccgcctg gcagtggcta cccataacct
     9241 ggcagccgct atgaggcacg ggctgctggc gctgatttgc tggctgtgtt gtgttgttgc
     9301 ccatagcgaa atgctgaatg tcgaacagtc ggggctgttt cgcgcgtggt ttgtacgcat
     9361 tgcacaggaa caactccgcc aggggccaag cccacgctgg tatcagcagg attgtgcggg
     9421 cctggtgcga ttttcggcga acgaggcgct gaaaattcac gacagcaaat ggctaaaaag
     9481 taacggtata gcaagtcagt atttgccgcc tgaaatgacg ctaacacctg aacagcgtca
     9541 actggcgcaa aactggaatc aggggaacgg aaaaaccggc ccctatgtga ccgcgattaa
     9601 tttgattcaa tataacagcc agttcattgg ccaggacata aaccaggcgc tgcctggcga
     9661 tatgattttt ttcgatcagg gcgatgccca gcacttaatg gtctggatgg ggcgttacgt
     9721 catctaccac accggaagcg ctacgaaaac tgacaacgga atgcgcgcag tcagtctgca
     9781 acaacttatg acatggaagg acacccgatg gatacccaac gattccaatc ccaatttcat
     9841 tggcatttat cgtttaaatt ttctggcgcg atagcagcgt gcttgtgcct gtctcttgtg
     9901 ggaacagggc ttgctaatgc tgatgattcg cttccttcca gtaactatgc gccgcccgcc
     9961 gggggaacat tctttttgct cgctgacagc agttttagca gcagtgaaga ggcgaaagtg
    10021 cgactggaag cgccggggcg tgattatcgg cgttatcaga tggaagagta cggcggtgtg
    10081 gatgttcgtc tgtatcgtat tcctgacccg atggcatttt tgcgccagca gaaaaatctg
    10141 catcgcattg tggtgcaacc gcaatatctg ggcgacgggc tgaacaacac gctgacctgg
    10201 ctgtgggata actggtacgg caaatctcgc cgcgtgatgc agcgtacttt ctcttctcag
    10261 tcacggcaga atgtgactca ggcattaccc gaattacatc tcggcaatgc cattattaaa
    10321 ccttcccgtt atgtacagaa caaccagttt tccccgctga aaaaatatcc acttgtggaa
    10381 cagttccgtt acccattatg gcaggctaaa ccggtcgagc cgcagcaagg ggtaaaactg
    10441 gaaggtgcat ccagcaattt catctcgccg cagccgggta acatctatat tccgctaggc
    10501 aagcaagagc cgggactgta cctcgtcgag gcgatggttg gtgggtatcg ggcgacgacg
    10561 gtggtgtttg tttccgatac cgtggcgctt agcaaagtgt caggtaacga gcttctggtg
    10621 tggaccgcgg gtaaaaaaca gggtgaagcg aagcccggct cagagatctt gtggactgac
    10681 ggtcttggcg tgatgacccg cggtgtgacc gatgacagcg gtaccttgca gttacaacat
    10741 atatcgccgg aacgttcata cattctgggt aaagatgctg aaggcggcgt ttttgtctcc
    10801 gagaattttt tctacgaaag cgaaatctac aacacccgct tgtatatctt taccgaccgc
    10861 cctctgtatc gtgcaggcga tcgtgttgat gtcaaagtga tgggccgcga gttccacgac
    10921 ccgttgcatt catcccccat cgtcagcgcc ccggcgaagc tttcagtgct ggacgccaac
    10981 ggcagtctgt tgcaaaccat caatgtcacg ctggatgcgc gtaatggcgg gcaggggagt
    11041 ttccgcctgc cagaaaatgc cgtcgccgga ggttatgagt tacgcctggc ttaccgcaaa
    11101 caggtctata gcagcagttt tcgcgtggca aactacatca agccacattt cgagattggt
    11161 ttagctctcg acaaaaaaga gttcaaaaca ggcgaagcgg tcagcggcaa actgcaactg
    11221 ctttacccgg atggagagcc ggtaaaagat gcccgcgtgc agttaagttt gcgatctcaa
    11281 caactgtcga tggtgggcaa cgatttgcgt tatgccggac gttttcccgt gtcgctggaa
    11341 ggcagtgaaa cggtgtccga cgatagcggt catgtggcgt taaatctccc cgccgctgat
    11401 aaaccaagcc gttatttgtt aaccgtctcc gccagtgacg gtgcggcgta tcgtgtcacc
    11461 accaccaaag agatcctcat tgaacgtggc ctggcgcatt attcattaag tactgccgca
    11521 caatacagta atagcggcga gtcggttgtg ttccgttatg ccgcgctgga atcttcaaaa
    11581 caggttcctg ttacgtatga atggttgcgt ctcgaagacc gcacgagcca tagcggagag
    11641 ctaccgtcag gcggcaaatc ttttaccgtc aatttctcta aacctggcaa ctacaatctg
    11701 acattacgcg atgaagacgg cttaattctc gctgggttaa gtcatgccgt cagcggtaag
    11761 ggcagcacgg cgcatactgg tacggtagat atcgtggcgg ataaaacgct gtaccagcca
    11821 ggcgaaaccg cgaagatgct gattactttc ccggagccaa ttgatgaagc gttattgacg
    11881 ctggaacgcg atcgcgtgga acagcagtcg ctgctttcgc atccggcaaa ctggctgacg
    11941 ttacaacgtt taaacgatac ccagtatgaa gcccgcgtac cggtgagcaa ttcctttgcg
    12001 cctaacatca ctttttcggt gctgtatacc cgtaacggtc agtacagttt tcagaacgcc
    12061 gggatcaaag ttgccgttcc ccagctggat atccgggtga aaacggacaa aacccattac
    12121 cagcctggtg aactggtcaa tgtcgaatta acctcttcgc tgaaaggtaa acctgtttct
    12181 gcgcagctaa cggttggtgt ggtcgatgaa atgatctacg cgctgcaacc ggaaatcgcg
    12241 ccgaataccg gcaaattttt ctatccgctg gggcgtaaca atgtgcgtac cagctccagt
    12301 ctgtcgttta tcagctacga ccaggcgctc tccagcgagc cggttgcgcc tggcgcgacc
    12361 aaccgcagcg agcggcgagt aaaaatgctt gaacgtccac ggcgtgaaga ggtggatacc
    12421 gcggcatgga tgccgtcact cacaaccgat aaacaaggca aagcgtattt cacgttcctg
    12481 atgcctgatt cgttaacccg ttggcgcatt acagccagag ggatgaacgg cgacgggctg
    12541 gtcgggcagg ggcgtgctta tcttcgttcg gaaaaaaatc tctacatgaa gtggagtatg
    12601 ccaacggtgt atcgcatggg cgacaaaccg gcagcaggac tgtttatctt cagtcagcag
    12661 gataacgaac cggtggcgct ggtgactaaa tttgcaggcg ctgagatgcg ccagacgctt
    12721 acgctgcaca aaggggcgaa ttatatttcg ctgacgcaga acattcagca atctggcttg
    12781 ttaagcgcag aactgcaaca aaatgggcaa gtgcaggaca gcattagcac aaaactgtct
    12841 tttgtggata acagctggcc cgttgaacag cagaaaaatg tcatgctcgc cggtggcgat
    12901 aacgcgctga tgttgcccga gcaggcgagc aatatccggc tacaaagtag tgaaacgccg
    12961 caggagattt tccgtaacaa tcttgatgcg ttagtcgatg aaccgtgggg tggcgtgatc
    13021 aacaccggta gccgtctgat cccgctcagt ctcgcctggc gttcgcttgc cgatcatcaa
    13081 agtgccgccg ctaacgacat tcgtcagatg attcaggata accgtctgcg gctgatgcaa
    13141 ctggcggggc ccggagcgcg ctttacctgg tggggtgaag atggcaatgg tgacgccttc
    13201 cttacggcat gggcatggta cgccgactgg caagcgagcc aggcccttgg cgtaacgcag
    13261 caaccggatt actggcagca tatgctcgac agttatgccg agcaggcaga taacatgccg
    13321 ttattgcatc gggcgctggt gctggcgtgg gcgcaggaga tgaatctgcc gtgcaaaacg
    13381 ttgttgaaag ggttggatga agctatcgcc cggcgcggaa ctaaaactga agatttctct
    13441 gaggaagaca cccgcgatat caatgacagc ctgatcctcg atacaccgga atctccactg
    13501 gcagatgcgg tggcaaacgt cttaaccatg atgttgctga aaaaagcgca gttgaagtcc
    13561 acggtgatgc cacaggttca gcaatatgcg tgggataaag cggcaaacag caatcagccg
    13621 ctggcgcaca cggttgtgct gctcaatagc gggggcgacg ctacccaggc ggctgctatt
    13681 ttaagtggtt tgaccgctga gcaatccact attgaacgcg cgctggccat gaactggctg
    13741 gcgaaatata tggcgacaat gcctccggtt gtgttgcctg cgcctgcggg cgcatgggct
    13801 aaacataagt taactggagg gggcgaatac tggcgttggg ttggtcaggg cgtgccggac
    13861 attctctctt ttggtgacga attatcgccg caaaatgtgc aggtccgctg gcgtgaaccg
    13921 gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg aacgccagtt gtatcggctt
    13981 atccccggtg aagaagagat gagctttact ctgcagccgg tgaccagcaa tgagattgac
    14041 agcgatgcgc tgtatctcga tgaaattacg cttaccagcg agcaggatgc cgttctgcgc
    14101 tacggtcagg tggaagtacc gctcccgccg ggagccgatg ttgagcgcac aacatggggc
    14161 atttcgatca ataaacccaa cgccgcgaaa cagcaggggc aattgctgga aaaagcgcga
    14221 aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag aactgacggg aacggtcact
    14281 ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg ttctgcctcc tgctcgttat
    14341 gtgcgttcct atgcacctgc acagcaacgt gttgcggcag ggagtgaatg gaccgggatg
    14401 caggtgaaat aagtgaactg gcgcagaatc gtctggctat tggcgttggt tactctgcca
    14461 acgcttgcgg aggagacgcc gttacaactg gtgctgcgcg gtgcgcagca cgatcaactg
    14521 tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat tacctgacac tctcaccacg
    14581 ccgttaggga gtttgtggaa actgtatgtt tacgcctggc tggaggatac ccatcaaccg
    14641 gagcagccct accagtgtcg tggaaactcc ccggaagagg tttattgttg tcaggctggg
    14701 gagagcataa cgcgcgatac cgcgctggta cgttcctgtg ggctctattt tgcgccgcag
    14761 cggttgcata ttggcgcaga tgtatggggg caatactggc aacagcgtca ggcacctgcg
    14821 tggttagcgt ctcttactac gctgaagcca gaagcctcgg taacggttaa atcactgctt
    14881 gattcattag cgacgttacc tgcgcaaaac aaagctcagg aagtcttact ggatgtggtg
    14941 ctggacgagg cgaaaatcgg agttgcatcc atgctcggta gtcgggtacg ggtgaaaacc
    15001 tggagctggt ttgccgatga taagcaggaa atccgtcagg gtggttttgc tggctggtta
    15061 accgatggta caccgctgtg ggttactggc agtggcacca gcaaaacggt actgacccgt
    15121 tatgcgacgg tgcttaatcg ggttttgcca gtgccgacgc aagttgccag tgggcaatgt
    15181 gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa tcacagcgga aaagagtacg
    15241 acggcagtaa aacccggcgt acttaatggg cgctatcgtg tgacgtttac taatggtaac
    15301 cacattacgt ttgtcagcca cggtgagacg accttactga gcgaaaaggg caaactgaaa
    15361 ttgcagtcgc atctcgatcg tgaagagtac gttgccagag ttctggatcg cgaggcgaaa
    15421 agtacgcccc ctgaggcagc gaaagccatg accgtggcga ttcgcacttt tttacagcaa
    15481 aacgccaatc gggaaggcga ttgcctgacc atccccgaca gcagcgccac gcagcgtgtt
    15541 tctgcttcgc cagcgaccac gggggcgcga acgatggccg cctggacgca ggatcttatc
    15601 tatgcgggcg atccggttca ttatcacggc agtcgcgcta ccgaagggac gctttcctgg
    15661 cggcaagcca tggcccaggc ggggcagggg gagcgttacg accagatact cgcgtttgct
    15721 tatcccgaca acagccttag ccgctgggga gcgccacgct caacctgtca gttattaccc
    15781 aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc ggcgtatatt acaaggcgag
    15841 acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt tagtctcagg tttcccctat
    15901 accgatcggc agcagaaacg gctgtttatt cgtaacttct ttacgcttca ggatcggctg
    15961 gatttaaccc atgagtatct gcacctggcc tttgatggtt atcccaccgg gcttgatgag
    16021 aactatatcg aaacgctgac ccgacaatta ttaatggact aatgctatgc gaaagatttt
    16081 tcttccgttg ttactggtgg cgctttcgcc tgtcgctcac agtgaaggtg tgcaggaagt
    16141 cgagattgac gcaccgctct ctggctggca tccggcagag ggcgaagatg ccagtttttc
    16201 acaatcgatc aattacccgg cgtcgtccgt caacatggca gatgatcaaa atatctcagc
    16261 gcagatccgc ggcaaaataa agaattatgc tgcggtgggt aaagttcagc agggccggct
    16321 ggtggtcaac ggtgccagta tgccgcagag aattgaatcc gatggttcat ttgcacgccc
    16381 ttatattttc actgaaggca gcaacagcgt gcaggtcatc agcccagatg ggcaaagccg
    16441 acaaaaaatg cagttttact caacgccggg cgctggagtg atccgcgcgc gtttacggct
    16501 ggttctctcc tgggatacgg acaataccga cctcgacctt cacgttgtta cacctgatgg
    16561 cgaacacgcc tggtacggta acaccgtgct gaaaaacagt ggtgcactgg atatggatgt
    16621 cacgacgggg tacggccccg agattttcgc catgccagcg ccgattcacg gacgttatca
    16681 ggtgtatatc aactactatg gcggacgcag cgaaacggaa ttaaccaccg cccaactgac
    16741 gctgatcacc gatgaaggat cggtcaatga gaaacaggaa acatttattg taccgatgcg
    16801 taatgctggt gagctgacgc tggtgaaaag ttttgactgg tgattaatgg ggttaccttc
    16861 gccgtcgatg ttcaagaaaa cgcccgataa ctttcgctat cgggtgtttt tattgattaa
    16921 ttcaaccgtt caatcaccat cgcaattccc tggccgccac caatgcacag tgttgccagc
    16981 cccagcgttt tatcgcgtgc ctgcatggca tgtaatagtg tgaccagaat acgagcacca
    17041 ctggcaccga taggatgccc gagcgcgatg gccccgccgt tgacattcac tttctcagaa
    17101 tcaaagccca gggttttccc aacggcaagg aactgtgcag caaatgcttc attagcctca
    17161 atgagatcga tatccgccag ttgcagcccc gccagttgta acgttttttg cgtggcaggt
    17221 actggcccca tacccatcaa tgcggggggc acgccaccgc tggcataact tttaatgcga
    17281 gccagggggg taaggcctgc tgccagcgcc gcagattctt ccataatcac cagagcggca
    17341 gcaccgtcgt taataccaga cgcgttcccg gcggtgactg ttcccgcttt atcgaaggcc
    17401 gggcgcaatg cgcctaacgc ttcagtcgta gaatccgctt tcgggaattc gtcttgactg
    17461 aagacgaagg ttttcttccg agtgacaaca tttaccggga cgatttcggc tgtaaaagca
    17521 ccggactcaa ttgcggctgc cgctttacgc tgtgaatgta gcgccagttc atcctgcatt
    17581 tctcgggtaa ttccgtactc tttggccacg ttctcggcgg taatccccat atgataacca
    17641 tgggtggcgc acatcagacc atcgcgcagg attacgtcat aaacctgtcc gtctccaaga
    17701 cgataaccag agcgtgcttt tgcatcgagt aagtagggcg ctaaactcat attttccata
    17761 ccccccgcca caatgctttg cgcctgacct gcctgaattg cctgggcggc aagcgccaca
    17821 cttttaagac ccgaaccaca cactttattg accgtgaatc cgcacaccgt ttctgccagc
    17881 ccgcttttta acagtgcctg acgtgccgga ttttgcccca acccggcttg caacacgtta
    17941 cccataatca cttcatcaat gtgtagtgaa tcgatttttg cacgttcaat ggcggcttta
    18001 attactgtcg cccccaggtc gatggcgctg gtggaagcga gtgaaccgtt aaaactaccg
    18061 atagcagtac gtaccgcact gacgatgaca caatttttca ttttttattc cttctgtctg
    18121 tagggttgcc gtcagaacag cgttaaacca acgacgaaaa tgacgccgga gaagagcagg
    18181 gcagtgatgc aatagcccat gatgtcgcgg acaccgagtc cggcgattgc cagtgctggc
    18241 agcgcccaga atggttgtgc catgttcatc cattgctcgc cgtaggcgat cgccattacc
    18301 gatttaccga gatcagcgcc cagcgcctgg gctgcgggta tcacgaaagg tccctgaata
    18361 acccagtgac cgccgccaga cggaacggcg aagttaatca gtgcagaact aaaaaaggtc
    18421 attaccggga aggtgtcttt gttcgcaaca ttgatgaaga attcggtaat gagtccgccc
    18481 agaccggaat gctccatcat cagttggatc ccagcgtaga aggggaattg cactagaata
    18541 ccggcagtac tgcgtgctgc cgcgctgata gcacgcatat aagccattgg cgttttatgt
    18601 agcagcagac ccgcaatcat aaacatcagg ttgacggtat tgatggtgat gttgaagcca
    18661 tgttcgctga agtacatcgc aaggtaagcg ataccgagtg cgccgatgat caacgtcaga
    18721 atgcggcttt cttccagtcg ctcggatggt ggggcatctt tcggtagctg cttttggaaa
    18781 tcagcctctt ccatgagtag tttcggatcg atactcacca cgtcagacgg ttttggcatc
    18841 atcatgcggg tgataaatgg catcaccaca atcaacgcca cagtgatgaa aatgttaaaa
    18901 ccactgaaca gagtatcgcc caccgggatc agcccggcga tatgctcaac cgggttgccc
    18961 ggtgttgcag ccaacagagg cattgagcca gagaaaccgc caccccaggt gagaaaacca
    19021 atgtaggcgc aggcaatgag caacggataa tcagaaccgg gtactcggcg ggcgacttca
    19081 cgggcaaaca ttgcgccgac aaccaaacca aatccccagt tgatgacaca agcgactgaa
    19141 ccgaagaaag taaccagcat gacgccctgt acgggcgtct ttgcggcgga ggcggcagta
    19201 cgcagcaaac tttttaccgg agcagagctg gcaagggcat gaccggtaac gatgataagc
    19261 gccatctgca taccaaacgc cagcaagttc cagaaaccgt caccccacat tttcaccatg
    19321 ctgatcggcg tttgtggtgt taaccaaagc gcgatcacga aagttagcaa tgtcagcaac
    19381 atggcaaaga tcagtggatc gggaagccac cggctgacaa aacgcgtcat aaaacgcgat
    19441 atgcgaccaa tcataaatca ccccgttgcg tattcagatc ggcggcaact tcaaaccgag
    19501 cttctgtttt ggcacacacg gtggctaaat cacacccgtc ggcaatttcg gtgagccaca
    19561 ttttgccgtc aataaaacga aagacagcca gttcagtaac cagcatatgc accgcatgtt
    19621 gcgcagtgag tggcatggtg cagcggcgca aaatttttgc tgaaccatct ttggcacaat
    19681 gttccatggc gatgatcact ttgcgcgacc cggtcaccag atccatcgcg ccacccatac
    19741 cgggcaccat tttcccaggc actacccagt tcgcgaggtt tgcttcttcg tctacttgca
    19801 aaccgccgag cacgcaggca tcaatatgac cgccgcggat tagcgcaaat gacatggcgc
    19861 tatcaaacat ggctgcaccg ggtaaaacac cgcacggttg tccgccagcg ttcaccagat
    19921 ctggatgcgc tgtcgtgacc gggcctaaac cgaggaagcc gttttccgat tgcagagtga
    19981 tatgaatacc ctccggtaaa taattggcga ccattgtggg taaaccgatc cctaagttaa
    20041 cgatgtcacc atcacgaagc tcttgcgcca cacggcgcgc aatacgttgt ttcgcatcca
    20101 ttatttgctc tcctgtgaaa cgatgatgtg gtcgataacg gcaccagggg tgacaatatg
    20161 gtcaggttgc agctcgccgg tttcgaccag ttcatctggc tctaccagcg tgatatcagc
    20221 cgcaagggct atcagggggt taaagttgcg ggcgctaagt tgataggtca ggttgccaag
    20281 tgtgtcgcaa cgatgagcgc gaattagcgc caggtcggcg cgcagtgggc gttcgagcag
    20341 ccagatttta ccgtcgagtg tcagtgtctg tttgccttcc tctacgacgg tgccgacacc
    20401 cgttggggtg agaaaaccac caagtccagc tccgccacag cgaatttgct cgattagcgt
    20461 accttgcggc accagaacga cgtccatctc accagatatc atgcgccgac ctgtttccgg
    20521 gttggtgccg atatgtgaag caatcacttt gcggactcga ccattgacga tgagcggacc
    20581 gatgccagta tcaacaaacg cggtatcatt ggctatcaat gtcaggtcgc gaacaccaga
    20641 ttccagtaat gcttcaacca ggcgggatgg agtgccaatc cccataaatc cgcccaccat
    20701 gatggtcatg ccgtcacgaa agaagccggt ggcatcttgt aatgtcatca attttgtttt
    20761 cattttttat ccctcttgca tacaccgttg tgggtacagt cagggaatag caaggggagt
    20821 gccagaatga gattattgga ttttgttatt taactaattg aatatgaagg gataaattgg
    20881 atggggattt ataagaagaa tgagcggcta tgcagaaaat tgcgcactgt gcaaatttct
    20941 gcatagcaag ttttggcgtt atacatccgc cggatcgata ccgtattcct ggagtttata
    21001 catcaatgca cggcgactga tgcccagcat taaagcagtg cgggttcggt ttccttcttg
    21061 ttgttccagc acttccataa tgatgcattt ttcgacgcgt ttaatttcct cttttaaatt
    21121 acgctctccg acaggggatg tttttacctc gccagcatta cagactggct gacgaatctg
    21181 cggcggaaga tcctcagaaa aaatgatcgg gcctgagttc atcacgacgg cgcgttcaat
    21241 aacgttggaa agctctcgaa tatttcccgg ccatgaccag gcggtaagca gtgacattgc
    21301 catcggatcg atgtcgataa tatcgcgctg attctcacta ctgaattttt gcaaaaagtg
    21361 attagctaac agggaaatat cttcccggcg atcgcgcaga ggcggcagta ttaaatgaat
    21421 aacgttaagg cgataaaaga gatcttcacg gaaggtgcct tcttttacca ttgcctgcaa
    21481 gtcacggttg gtggcagcaa tgatgcggat atcaactttt atggtctgat ggccgccaat
    21541 ccgttcaaat tcccgttcct gcagaatgcg tagtaatttg gcttgcagta cgagcggcat
    21601 ttcgccaatt tcgtcgagga gcagagtacc ttcgttggca cgttcaaata atccctgacg
    21661 caaggtttgt gcaccagtaa atgcaccttt ttcatgacca aacagttcac tttcgagcaa
    21721 cgattccggc agcgccgcgc agttgacttt aatgaacggc ccctttgccc gccgcgaatt
    21781 gtagtgaatc gctctggcaa tcagctcttt cccggtgccg ctttcaccgc taatcaagac
    21841 gctggcctga gaaagggcaa ttttggcggt gtctttgcag atgtccatca tcgccgggct
    21901 gttggtgaga atgtgccccc attgccagct agtgctcagt gcctggtgca gatggcggat
    21961 ctcttttttc attgactgga gttgtaaagc gcgctgaacg attaaattca actcatcgag
    22021 atcaaacggt ttgataacat agtcgaaggc cccgcagcgt agcgcttcga cggcggtttc
    22081 cacttcagca taggccgtca tcagaataac gggggtccgt gtctcatggc tgcgcatctc
    22141 cttgagtgcc ttgatgccgt ccatctctgg catgcggata tccatcaaca ccacgtcagg
    22201 gtgaatatcg gcaaacaggt gtaatgctgt gcgtccgttg ttcgcacaat gtgtttcgaa
    22261 tccttgtagt gcaaaagcgg tgctcagcat acggcgaaca ttatcttcat catccacaat
    22321 aaggatgcga ttaatagcag tcatacagtc tgatttccct gcggattgat cggtaaaata
    22381 agcgtgaagg ttgcgccgta gcccggcaaa ctggcgacgc gaatatcacc ctgatgggca
    22441 ttaatgatgc gttgacttaa cgccagacca agcccggttc ctgaggcttt ggtggtgaaa
    22501 aagggatcga agatcttttt ttgcagcgag agatcaatgc cacagccgtt gtcctctatc
    22561 gaaatggcct gttgtgagtc gctgtattgc caggtttgaa tgcgaatttt ccctcgtgcg
    22621 ctgatagcct ggacggcatt gatcaggata ttcagtagta cctgtttgag cagttcacga
    22681 tcggcgttaa tcgggtttaa ttcattatcc agttcgctta tgaagtcgac ccgagcttgt
    22741 acgccggcgg tttgtaccag taccagagtt tcttcaacca atgcattgag actgacttgt
    22801 tgccattgac tgtggcgtgg acgtgaaaat tcgagcaatt gctgaataac tttgttaatt
    22861 gaatcgattt ctttgagtac tacggacaga tattcctgat gtattaggtc gctggtttgt
    22921 tggcgcaaga tctgtacata accacgaata gccgttagcg gattacgtac ttcatgcgcg
    22981 acgccagcca tcagctcacc cagtgtggcg aggcgttctg cttgcgccat gcggcgctgg
    23041 gtttctttgc gggcagttaa atcagagaaa atcaccaaag cacctatcat ttcaccatgc
    23101 gtgttatgaa tacgactggt agtgacactg agttcaatgg tgcggtcacg gcctggaaaa
    23161 ctgatctcca gcgctacatg ttcggtgcca tgttccagcg tatccagtac tggactgtag
    23221 aactgagtat tgtcgaacaa catggagtaa ggctgcccta ccagttcatg acgttgatag
    23281 ccagtgatga cttctgctgc cgggttcatg gtggttacat caccctggcg gtcaatggca
    23341 atgacgccat cggcagcgtt ttcaataatc agatcgttaa gtgtccgcgt ttcacgcagt
    23401 gcctgggcga ggttgttaac actctgactg atttgcccca tttcaccggg caattgtggt
    23461 aatcgagtgg gaatattttg cgccagagtc gagaggccat cggtgatgat atcaatattg
    23521 gcgctcaggc gacgggagaa aaggacaatc aacagcaggc ttatcagcaa accggcggtg
    23581 agcacaatga taatcctcac atccattttc caggcctggc ggcgaatatc ttcggttaat
    23641 tcattggccc agatatagcc gaggatttca ccattacgct caatggggat cattgaattc
    23701 aaaatatcgc cgcgcacctg cctgcctgaa taaaccaaag gggtatttgt acgcatgact
    23761 tcgcgaccag ggtgatctgc ggcaatggta acgcccacat tattctgata tagcgctgaa
    23821 ggtgcgtagg tgattatcgc atccagcgtt ttgttgtaat aaccagcgcc gatgccaggg
    23881 aaggcgtgag tgatattttc ggtaatgggg gcaagttctg catttaatgc gcggatacgc
    23941 tcctcacgcg gtaagtcgat gtagagatcg tagcgattgc ctagtgcctg attaagcagg
    24001 ttgaccacgg cagaaagttt tttctctttt tcagataaga ctgctgaacg tccttccgtt
    24061 tcaacgatat aaccaatagt aagcgttggg acaatgacca tcaggattgc catcaggatc
    24121 atttgattgc gtaagcggcg tggataaatc cacttcatat aatgcatgct gaaatccact
    24181 agtcttgtcc ggtatatgac gattatcaga ggttaaggtg atgatttctc ggcggtgtat
    24241 catattccag agaagagaga acattgcggt aacacgcttt taccgctacc ttaaccacac
    24301 tccatcggtc acctgaggcg gagcttcgcc cctttgaaat accttgcttc ttttcgtaca
    24361 accctgaaag cctcgcgcta catgttcaga gcattggcgt tagtgctctg gctgttgatt
    24421 gctttttcat ccgtttttta catcgttaat gcgttacatc agcgagaatc ggaaattcgt
    24481 caggaattta atctgagttc cgatcaggct cagcgcttta ttcaacgcac ctctgatgtg
    24541 atgaaagagc tgaagtacat agctgaaaat cgcttatcgg cagaaaacgg tgtgctttcc
    24601 ccgcgtggac gagaaacgca gacggatgtg cctgcgtttg aaccgctgtt tgctgactct
    24661 gattgttccg caatgagtaa cacctggcga ggctctctgg agtcattggc gtggtttatg
    24721 cgctactggc gcgataattt ttctgcggct tacgatctca accgggtatt tttaatcggc
    24781 agcgataacc tttgcatggc caatttcggt ctgcgtgata tgccagtgga acgcgatacc
    24841 gcgttgaaag ctttgcatga acgcatcaat aaatatcgaa atgcaccaca agacgatagc
    24901 ggcagtaacc tctactggat cagcgaaggt ccgcgccctg gcgtcgggta tttttacgcg
    24961 ttgacgccag tttatctggc gaaccggttg caggcgcttt tgggtgtcga gcagaccatc
    25021 cggatggaga actttttctt acctggtacg ttgccgatgg gggttaccat tcttgatgaa
    25081 aatggtcata ccctgatttc gcttaccgga ccagaaagca aaattaaggg cgatcctcgc
    25141 tggatgcagg aacgctcctg gtttggctat acggaagggt tccgggagct ggtgctgaag
    25201 aaaaatctgc caccctcatc gctaagcatc gtttattcgg tgccagttga taaggtgctg
    25261 gaacgcattc gcatgttgat ccttaacgca attttgctga atgtgcttgc cggagctgca
    25321 ttgtttactc tcgcgcggat gtacgagcga cgtattttca ttccggcgga aagcgacgcc
    25381 ctgcgactgg aagaacatga gcagtttaat cgcaagattg tcgcctccgc gcccgtgggt
    25441 atctgcattt tgcgtaccgc tgatggcgtc aatattttaa gtaacgaact ggcgcatacc
    25501 tatctcaata tgcttacgca tgaggatcgc caacggctga cacaaattat ctgtgggcag
    25561 caggtcaatt ttgttgatgt cctgaccagc aacaatacca atctgcaaat cagcttcgtc
    25621 cattcgcgct atcgtaatga aaacgtggcc atttgtgtgc tggtggatgt ttcttcgcgc
    25681 gtgaagatgg aagagtcgtt gcaggagatg gcacaagcag cggaacaggc gagccagtca
    25741 aaatcgatgt tccttgccac cgtcagtcat gagctgcgaa cgccgctgta tggcattatc
    25801 ggtaacctgg atcttttgca aaccaaagag ttaccgaaag gcgtcgatcg tctggtgacg
    25861 gcaatgaaca actcttccag cctgttgttg aaaattatca gcgatattct cgatttctcg
    25921 aagattgaat cggaacagtt gaagatcgaa ccgcgtgagt tttcaccgcg tgaagtgatg
    25981 aaccacatca ccgccaacta tttaccgctg gtggtacgca agcagctagg cttgtactgc
    26041 tttattgaac cggatgtgcc agtggcctta aatggcgacc cgatgcgttt acagcaggtc
    26101 atctccaatc tgttgagtaa cgccataaaa ttcaccgata ccggctgtat agttctacat
    26161 gttcgcgctg atggcgatta tctctctatc cgtgttcgcg ataccggcgt ggggattccg
    26221 gcgaaagaag tggtgcgctt gtttgatccc ttcttccagg tcggaacggg cgtacagcga
    26281 aacttccagg ggaccggtct gggtctggcg atttgtgaaa aactgatcag catgatggac
    26341 ggcgatatct cggtagattc agaaccggga atgggcagcc agtttaccgt gcgtattccg
    26401 ttgtacggcg ctcagtaccc gcagaaaaaa ggcgtggaag ggttgagtgg taaacgctgc
    26461 tggctggcgg tccgcaatgc gtcgctctgt cagttcctgg aaaacagttt gcagcgcagc
    26521 ggcatcgtcg taacaacata cgaagggcag gaaccgactc ccgaagatgt gttaatcact
    26581 gacgaggtag tgagtaaaaa atggcagggc agagcggtag tgaccttctg tcgtcgacat
    26641 attggtattc cgctggagaa agcgccagga gagtgggtac acagtgtggc tgctccgcat
    26701 gagctaccgg cattgttggc gcgtatttat ttgatcgaga tggagagcga cgatcctgct
    26761 aacgctctgc cgtcgacgga caaagcggtc agcgataatg acgatatgat gattctggtc
    26821 gtggatgatc atccgattaa ccgacgtctg ctggcagatc agttgggatc gttgggctat
    26881 caatgtaaaa ccgcgaatga tggcgtcgat gcgcttaatg tacttagcaa gaatcatatt
    26941 gatatcgtgc ttagcgacgt caacatgcca aatatggatg gttaccgctt gacgcaacgc
    27001 attcgtcagt tgggactgac gttgccggta atcggagtaa ctgctaatgc gttggctgaa
    27061 gagaagcagc ggtgtctgga gtccggtatg gacagctgcc tgtcgaagcc ggtaacgctg
    27121 gatgtgataa aacagacgct gacggtatat gccgagaggg tcaggaaatc gcgggaatcg
    27181 taggctgtat aagacgttta cgccgcatct ggcattcagt gcaaatgcca gatgcgacgc
    27241 tggtgcgtct tatctggcct actttgttgt aatccattga aataatggga atcgtaggcc
    27301 ggataaggcg tttacgccgc atccggcatt cagtgcaaat gccagatgcg acgctgacgc
    27361 gtcttatctg gcctacaggt gattagtctt tatctgccgg acttaaggtc acagaagaga
    27421 gataattcag cagagcaata tcgttctcga cacccagctt catcatcgca gatttcttct
    27481 ggctactgat ggttttgata ctgcggttca gctttttagc gatctcagtc accaggaaac
    27541 cttccgcaaa caggcgcaga acttcactct cttttggcga gagacgcttg tcaccgtaac
    27601 caccagcact gattttttcc aacaggcgag aaacgctttc cggggtaaat ttcttccctt
    27661 tctgcagcgc ggcgagagct ttcggcagat cggtcggtgc accttgtttc agcacgatcc
    27721 cttcgatatc cagatccaat accgcactaa gaatcgccgg gttgttgttc atagtcagaa
    27781 caatgatcga caggcttggg aaatggcgct tgatgtactt gattaaggta atgccatcgc
    27841 cgtacttatc gccaggcatg gagagatcgg taatcaacac atgcgcatcc agtttcggca
    27901 ggttgttgat cagtgctgta gagtcttcaa attcgccgac aacattcacc cactcaattt
    27961 gctcaagtga tttgcgaata ccgaacaaga ctatcggatg gtcatcggca ataattacgt
    28021 tcatattgtt catgtaatag gctaccttgc tacagcaagc tcttgacata actgtcaatg
    28081 tcgctgatgt atttttctat tcctggaaca tccttctcac gaatcagatg ttccagcgtt
    28141 tcacataact gcttgccggg taccagattt agcatggcaa atacgccttt aagacgatga
    28201 gccgtttggg ctaacgcagc aaagtcactg gttgctgctt cagtatacag cctcttaaca
    28261 tcatccggta ctgtgtctac aaagagcgca taatagccgc tggcatggag ttgcgcattt
    28321 tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg ccagttgcac ttcaattaat
    28381 tgtaagaccg cttcctgcat agcgttgctc atattgaagt tgacgcacaa ttgaccaggc
    28441 ccaatttccc gtacgccaga ctcatcatcg cttaaaagca agccagaggc agtaagatta
    28501 gacggattat ccgttaaaaa gatatcataa tcttgactaa ttaatctttc atcgggtgtg
    28561 atacaggttg caccccaatt ttctaactgg cgagtgacaa tattccgaat ttctgccgaa
    28621 gtaacatcca ccattacgca gacatcatcc agtaaacgct cttcttcctc ttcaacttcc
    28681 gggtcagctg cgagcatttt gatatgcaca gagtagcgtg taccaagccc atcccgcgtt
    28741 ttaatgttta aatggccgcc cagtttacgt gccagttgat cgctcagcca gaatgccagc
    28801 gggtccgcct tgccatagcg atcgttttgg gtctggttga taaacgggaa gtgcaaatta
    28861 tccatttcat gaatacttac gccttctccg gtgtccagaa tgcggaacgt caggcggtct
    28921 tcggaggact catcctgatc aacctcaagg gtgatttttc ccaattgtgt tgaggtcacg
    28981 gcatattgca tcagtagcag caaaatgcgt cgtaaggcat cgcgatcgcc gcggcgcata
    29041 tcgtgtgctt tcagatgatt gttaatcagc agttgcagac ctttacgctt gatggcaggc
    29101 aacactgaag gcacaacttc atcaatcaaa tcctgcacag agaacagcac cgtctcactt
    29161 ttccagctat cgtccgcaag catgttcgct aactgtattt catcgaccag ccgcaccagc
    29221 acatctgcct gattcgccag ttgtttgctt tccggggcgt tgagtttagc tgcgctctcc
    29281 gccagggact gtgcaggttc tttcagcgca tcgccaatgt ttttcataaa ggtcatccgc
    29341 ccctgctggt ttttctcata cagacgctgc gcctgcttga gtttcttgtt taccagcact
    29401 tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg gcgcgacctg gctgcggaac
    29461 atgcggatct catacagctc gttattgatc gtcgcctgaa taatcccctg atgttgttcc
    29521 gccatggtgg tgatgttttg cagattcaaa tgcggcagca aatgatcggc aattttgtta
    29581 cttatgacag tgcggttcga ttcctgatcg tgaaccagca ggccgagcgg cagcagtgag
    29641 actatctctt cattaattgc ccgtaaaatg cgcaattcgt tattgaccgc cgtgctgggg
    29701 acgctttctg tactgcggct ggagaaatgg cggaatgtgg tatagccaaa taacgccagc
    29761 gccagcaaac cgatgttcag cagcagtggc agcagaatgt tttgcaacgt atccagcaat
    29821 aaggtgccat aaggaacctg ccagaccagt cgcatatcgg tagagttgag tgccgaggag
    29881 atttcaatct tcgtactgtt aaagtggata ctgacgctat ccgtcccttc tttctcatta
    29941 tcattgtttc ccgttgccgt cgcgtctggc tcaaggcgga aactgtccag cggcataccc
    30001 ggcgggatca aatcattaat cggcagatca aaagccacga ccgttgccag atgtcctggc
    30061 tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct gccaggccag gcggcgcatg
    30121 ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt gcaacatctc tgcacgacgt
    30181 gaatcaacaa tgtcgctgac ggtcgattct ttaaatccgg aggtgagatc tttgaggggc
    30241 agggttgaga tcagcaccag actgttatcc tgaccattca ggtaatacat cgaccacggt
    30301 acattttctg cgccccacaa tgtgtccaga taggtggaca tccgttgagt catctcaagc
    30361 gttgagttgt cgtgagagcc aaagattaac gcttccgttt tgcggcgtgg tttttccaga
    30421 tagtagacat cctgtttcag gcgcgtctct tgtaaacctt cgccggagga gggggaggtc
    30481 gtcgcggcaa tgttgtcgta aatctgccag gtcacgtaac gccaggtatc gacgcgcttt
    30541 tggattgcgt gggtaatgtc gacaatctgg taacttttat ctttcagcca ggcgttaacg
    30601 gcactttgta ccattacccc catcgtcacc agtaacacaa tgatcaacag taaaaagaag
    30661 cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg ccgttgtctc tttctgacgc
    30721 attcgtgttt atgacctgtt aaaacttcgc gaagtgatag ggcagtataa agggtacagt
    30781 gtgaaattcc agactcttac ctgattgatg attttctatt ctcccgtgat atttatcatc
    30841 ctgcattggg ggaaatagat gtacaagcgc catttcgctg tacaaataac ctacaaaaag
    30901 cccaacgaaa ttaattgcaa ataaacgaag attgctggaa attatgctga tgttaattat
    30961 ttgtgaaata gttaacaagc gttatagttt ttctgtggta gcacagaata atgaaaagtg
    31021 tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg ggtaaacaga
    31081 cattcagaaa tgaatgacgg taaaaaataa agttaatgat gatagcggga gttattctag
    31141 ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa atacttaaga ataagttatt
    31201 gattttaacc ttgaattatt attgcttgat gttaggtgct tatttcgcca ttccacaata
    31261 atcttaaaaa gttcccttgc atttacattt tgaaacatct atagcgataa atgaaacatc
    31321 ttaaaagttt tagtatcata ttcgtgttgg attattctgc atttttgggg agaatggact
    31381 tgccgactga ttaatgaggg ttaatcagta tgcagtggca taaaaaagca aataaaggca
    31441 tataacagag ggttaataac atgaaagtta aagtactgtc cctcctggtc ccagctctgc
    31501 tggtagcagg cgcagcaaac gctgctgaag tttacaacaa agacggcaac aaattagatc
    31561 tgtacggtaa agtagacggc ctgcactatt tctctgacga caagtctgta gatggcgacc
    31621 agacctacat gcgtcttggc ttcaaaggtg aaactcaggt tactgaccag ctgaccggtt
    31681 acggccagtg ggaatatcag atccagggca acagcgctga aaacgaaaac aactcctgga
    31741 cccgtgtggc attcgcaggt ctgaaattcc aggatgtagg ttctttcgac tacggtcgta
    31801 actacggcgt tgtttacgac gtaacttcct ggaccgacgt actgccagaa ttcggtggtg
    31861 acacctacgg ttctgacaac ttcatgcagc agcgtggtaa cggcttcgcg acctaccgta
    31921 acactgactt ctttggtctg gttgacggcc tgaactttgc tgttcagtac cagggtaaaa
    31981 acggtagcgt aagcggcgaa ggcatgacca acaacggtcg tggtgctctg cgtcagaacg
    32041 gcgacggcgt tggcggatct atcacttatg attacgaagg cttcggtatc ggtggtgcga
    32101 tctccagctc caaacgtact gatgatcaga acagcccgct gtacatcggt aacggcgacc
    32161 gtgctgaaac ctacactggt ggtctgaaat acgacgctaa caacatctac ctggctgctc
    32221 agtacaccca gacctacaac gcaactcgcg taggttccct gggttgggcg aacaaagcac
    32281 agaacttcga agctgttgct cagtaccagt tcgacttcgg tctgcgcccg tctgtagcat
    32341 acctgcagtc taaaggtaaa aacctgggca ccatcggtac tcgtaactac gacgacgaag
    32401 atatcctgaa atatgttgat gttggtgcta cttactactt caacaaaaac atgtccacct
    32461 atgttgacta caaaatcaac ctgctggacg acaaccagtt cactcgtgac gctggcatca
    32521 acactgataa catcgtagct ctgggtctgg tttaccagtt ctaatctcga ttgatatcga
    32581 acaaagggcc tgcgggccct tttttcattg ttttcagcgt acaaacacag ttttttggtg
    32641 tactcttgcg accgttcgca tgaggataat cacgtatgga aataagcttt acccgcgttg
    32701 cactgctggc tgccgcgctc ttctttgttg gttgcgatca aaaaccacaa cccgccaaaa
    32761 cccacgctac tgaagttacc gttcttgaag gcaaaactat gggtaccttc tggcgtgcca
    32821 gcatcccggg cattgacgcc aaacgcagtg ccgaacttaa agaaaagatt cagacccagc
    32881 tggacgccga cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg atgcgattta
    32941 acgactcgca aagtttatcg ccgtggccgg taagtgaagc gatggccgat atcgtcacta
    33001 cctcgctacg cattggcgcg aagaccgatg gcgcgatgga tataaccgtc gggccgctgg
    33061 tgaatctgtg gggtttcggt ccggaacaac agccggttca aattccgagc caggaacaga
    33121 tcgatgcgat gaaagccaaa accggcttac agcacctgac ggtgattaat cagtcccacc
    33181 agcaatatct gcaaaaagac ctgccggatt tatatgtcga tctctctacc gtcggtgaag
    33241 gttatgcggc ggatcacctg gcacgcttga tggagcagga agggatttcc cgctatctgg
    33301 tgtcggtggg cggcgcgctg aacagccgtg gtatgaacgg tgcaggccag ccgtggcggg
    33361 tagcgattca aaaaccaact gataaagaaa atgcggttca ggccgtggtg gatatcaacg
    33421 gtcatggcat tagcacctct ggcagctacc gtaactatta cgaactggat ggcaaacgtc
    33481 tttctcatgt tatcgatccg caaaccgggc gtcccatcga acacaatctg gtatccgtga
    33541 cggtgattgc cccgacggcg ctggtagccg atgcctggga tactggcttg atggtactgg
    33601 ggccggagaa agccaaagag gttgttcgtc gggaagggct ggcggtttat atgatcacca
    33661 aagagggcga tagttttaaa acctggatgt cgccgcagtt taaaagcttc cttatcagcg
    33721 aaaaaaatta aagcgcaaga ttgttggttt ttgcgcgatg gtgaccgggc agactaaagg
    33781 ctatccttaa ccagggagct gattatgaaa aacgccacat gcttaactga cgatcaacgc
    33841 tggcaatctg tcttagcccg cgacccgaat gccgacggcg aattcgtttt cgccgtgcgc
    33901 accacaggca tcttttgccg tccgtcttgc cgcgccagac atgctttgcg ggaaaacgtc
    33961 tccttctacg caaatgccag tgaggcactc accgccggat ttcgcccctg caaacgctgc
    34021 caacccgata aagccaatgc ccagcaacat cggttggata aaatcaccca cgcctgccga
    34081 ctgctggaac aggaaacgcc tgtaacgctg gaaaccttag ccgaccaggt ggcgatgagc
    34141 ccgttccatc tgcatcggtt gtttaaagcg actaccggaa tgacgccgaa agcctggcaa
    34201 caggcctggc gcgctcgccg tttgcgcgaa tcgctggcga aaggggagag cgtgactaca
    34261 tctattctta acgccggatt tcccgacagc agcagttact accgcaaagc cgacgaaacg
    34321 ctgggcatga cggctaaaca attccgtcac ggtggcgaaa atctggcggt gcgttacgcg
    34381 ctggctgatt gtgagctggg ccgttgcctg gtggcagaaa gcgagcgggg gatttgcgcg
    34441 atattgctgg gcgatgatga cgcgacatta atcagcgagt tgcagcagat gtttcccgct
    34501 gccgacaacg cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt gatcgccagc
    34561 ctcaatcaac gcgatacgcc gctgacgtta ccgctggaca ttcgcggcac tgcttttcag
    34621 caacaagtct ggcaggcatt gcgcacgata ccttgcggtg aaaccgtcag ttatcagcaa
    34681 ctggctaacg ccatcggcaa accgaaagcg gtacgtgccg ttgccagcgc ctgtgccgcc
    34741 aacaagctcg ctatcgttat accttgtcat cgggtggtcc gtggtgatgg cacactttcc
    34801 ggttaccgct ggggcgtgtt gcgtaaagca caactgctgc gccgcgaagc tgaaaatgag
    34861 gagagataat gttggatctg tttgccgatg ctgaaccgtg gcaagagcct ctggcggctg
    34921 gtgcggtaat tttacggcgt tttactttta acgctgcgga gcaactgatc cgcgatatta
    34981 atgatgttgc cagccagtcg ccgtttcgcc agatggtcac tccaggcgga tataccatgt
    35041 cggtggcgat gaccaactgt gggcatctgg gctggacgac ccatcggcaa ggttatctct
    35101 attcgcccat tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg
    35161 atttatgtca acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc
    35221 ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg
    35281 atctgcgcgc gccgattgtt tctgtttctc tgggcttacc cgcgattttt caatttggcg
    35341 gcctgaaacg aaatgatccg ctaaaacgtt tgttgttgga acatggcgat gtggtggtat
    35401 ggggcggtga atcgcggctg ttttatcatg gcattcaacc gttgaaagcg gggtttcatc
    35461 cactcaccac cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa
    35521 aataagaatt attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg
    35581 tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta
    35641 tcagtgtgat ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta
    35701 tcaatcagcg tcttatcgaa acggcagata ccagtctgct ggtgttgccg gagtttctgg
    35761 gattattgct actgttgatg gctgtcactc tcggatcgca actggcgctt actacgctgg
    35821 gccatcactt cgtttaccga ctgcgtagcg aatttatcaa gcggattctg gatacccacg
    35881 tcgagcgcat tgaacaactt ggtagcgctt cgttgctggc gggattaacc agcgatgtgc
    35941 gcaatatcac cattgctttt gtgcgtctgc cggaactggt gcaggggatc atcctcacta
    36001 tcggttccgc ggcgtacttg tggatgctgt cgggcaaaat gttgctggta acggctatct
    36061 ggatggcagt caccatctgg ggcggttttg tgctggtggc gcgggtgtac aaacatatgg
    36121 cgaccctgcg tgaaaccgaa gacaagctgt acacggattt tcaaaccgta ctcgaagggc
    36181 gcaaagagct gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacattc
    36241 ctgatgcgca agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg
    36301 tgaactggtc aaacatcatg atgctgggcg caatcggcct ggtgttctgg atggcgaaca
    36361 gtctcggttg ggctgatacc aacgttgccg cgacctattc gttgacgctg ttattcctgc
    36421 gtacgccgct gctttcggcg gttggcgcat taccgacgct gctgacggcg caggtggcgt
    36481 ttaacaagct gaacaaattc gcgctcgcac ctttcaaagc agagtttcca cgcccgcagg
    36541 cgtttcccaa ctggcaaacg ctggagctgc gtaacgtgac gtttgcttat caggataatg
    36601 cgttttccgt tgggccgatt aacctcacca ttaaacgtgg cgagctgctg tttctgattg
    36661 gtggcaacgg tagtggaaaa tcgacgctgg cgatgttgct gacgggcttg tatcagccac
    36721 aaagcggcga aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc
    36781 gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt cgatcaactg ctggggccgg
    36841 agggtcaacc cgctaacccg caactgattg agaagtggct ggcgcaactg aaaatggctc
    36901 ataagcttga gttaagcaac gggcgtattg ttaacctgaa gttatcgaaa gggcagaaaa
    36961 aacgcgtcgc gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat
    37021 gggcggcgga tcaggaccca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga
    37081 tgcaggagat gggtaaaacg attttcgcca tcagtcatga tgatcattac tttatccacg
    37141 ccgaccgcct gctggaaatg cgcaacgggc aacttagcga gctgacgggc gaagagcgcg
    37201 atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg
    37261 actgctgccg ccaggtcaat atgctaaaaa tcccttcatt tctaat
//
LOCUS       DAYPHN010000030        35671 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000030 DAYPHN010000000
VERSION     DAYPHN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.030"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            90..533
                     /gene="cmtB"
                     /locus_tag="ACG0BW_001455"
     CDS             90..533
                     /gene="cmtB"
                     /locus_tag="ACG0BW_001455"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFK7258439.1"
                     /translation="MRLINYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTMSNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            561..1949
                     /gene="cmtA"
                     /locus_tag="ACG0BW_001456"
     CDS             561..1949
                     /gene="cmtA"
                     /locus_tag="ACG0BW_001456"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFK7258440.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1964..3241
                     /gene="yggP"
                     /locus_tag="ACG0BW_001457"
     CDS             1964..3241
                     /gene="yggP"
                     /locus_tag="ACG0BW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFK7258441.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3238..4203
                     /gene="glpX"
                     /locus_tag="ACG0BW_001458"
     CDS             3238..4203
                     /gene="glpX"
                     /locus_tag="ACG0BW_001458"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFK7258442.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRQAAGAIDLSLPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSTAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKEMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4225..4734
                     /gene="fumE"
                     /locus_tag="ACG0BW_001459"
     CDS             4225..4734
                     /gene="fumE"
                     /locus_tag="ACG0BW_001459"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFK7258443.1"
                     /translation="MATLTEDDVLEQLEAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQN
                     EMPGFADDMTWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4731..5444
                     /locus_tag="ACG0BW_001460"
     CDS             4731..5444
                     /locus_tag="ACG0BW_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFK7258444.1"
                     /translation="MKIELTVNGLKVQAQYHVDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADADAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYHLVD"
     gene            complement(5416..6093)
                     /locus_tag="ACG0BW_001461"
     CDS             complement(5416..6093)
                     /locus_tag="ACG0BW_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7258445.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLPPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWMSVFESWLEKCRQRGTSVIAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(6087..6764)
                     /locus_tag="ACG0BW_001462"
     CDS             complement(6087..6764)
                     /locus_tag="ACG0BW_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7258446.1"
                     /translation="MVTLEQFSYCPTHSTHPPFCYDFHYVKSGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEEEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6752..7459)
                     /locus_tag="ACG0BW_001463"
     CDS             complement(6752..7459)
                     /locus_tag="ACG0BW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFK7258447.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQFVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAIRGAALDMRAFRIHNRRTTLWAPADSPLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7460..8038)
                     /locus_tag="ACG0BW_001464"
     CDS             complement(7460..8038)
                     /locus_tag="ACG0BW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFK7258448.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8061..8492)
                     /locus_tag="ACG0BW_001465"
     CDS             complement(8061..8492)
                     /locus_tag="ACG0BW_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFK7258449.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYGGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8864..9883
                     /gene="epd"
                     /locus_tag="ACG0BW_001466"
     CDS             8864..9883
                     /gene="epd"
                     /locus_tag="ACG0BW_001466"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFK7258450.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9933..11096
                     /gene="pgk"
                     /locus_tag="ACG0BW_001467"
     CDS             9933..11096
                     /gene="pgk"
                     /locus_tag="ACG0BW_001467"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFK7258451.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11310..12389
                     /gene="fbaA"
                     /locus_tag="ACG0BW_001468"
     CDS             11310..12389
                     /gene="fbaA"
                     /locus_tag="ACG0BW_001468"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFK7258452.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12453..12551)
                     /locus_tag="ACG0BW_001469"
                     /pseudo
     CDS             complement(12453..12551)
                     /locus_tag="ACG0BW_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12747..13622
                     /gene="mscS"
                     /locus_tag="ACG0BW_001470"
     CDS             12747..13622
                     /gene="mscS"
                     /locus_tag="ACG0BW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFK7258453.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKENKENEAVTR"
     gene            complement(13664..14707)
                     /locus_tag="ACG0BW_001471"
     CDS             complement(13664..14707)
                     /locus_tag="ACG0BW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFK7258454.1"
                     /translation="MHYRQLLPFLLSGFLLLSPSIFAAEKILRYTDHEPYGGMRTQII
                     KEIFFAEIEKESQGRLKIEPHWNGETAISYDALTTISDGSKADMGIVVPEYTAKQLPL
                     HQIFKSFAIGPDHGASQVEFFRRVYAEIPEFNAELERNNIVNLQFFLGYPVGFFSTRP
                     IDKLTALQGTTWRTASFWHRAYLTHTGAKTVTLPWNDQITKALMDGKLDGLMVNLDSG
                     YDIHAERAAPNVLLSPSLWLGHVYLLVMNKQSWENLDNRDREAIQRAAITTEKALGKA
                     LDNNLISMVKTLEQEGAQVRYLKKSGLDAWQKAIGYQQEQAQWVEKQNKEGVEKAGEV
                     MQKVANILDETMR"
     gene            14823..15458
                     /gene="argO"
                     /locus_tag="ACG0BW_001472"
     CDS             14823..15458
                     /gene="argO"
                     /locus_tag="ACG0BW_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFK7258455.1"
                     /translation="MISYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            15551..16291
                     /gene="yggE"
                     /locus_tag="ACG0BW_001473"
     CDS             15551..16291
                     /gene="yggE"
                     /locus_tag="ACG0BW_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFK7258456.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            16459..17355
                     /gene="ygfI"
                     /locus_tag="ACG0BW_001474"
     CDS             16459..17355
                     /gene="ygfI"
                     /locus_tag="ACG0BW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7258457.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(17352..18830)
                     /gene="scpC"
                     /locus_tag="ACG0BW_001475"
     CDS             complement(17352..18830)
                     /gene="scpC"
                     /locus_tag="ACG0BW_001475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFK7258458.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(18854..19639)
                     /gene="scpB"
                     /locus_tag="ACG0BW_001476"
     CDS             complement(18854..19639)
                     /gene="scpB"
                     /locus_tag="ACG0BW_001476"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFK7258459.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQAFSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(19650..20645)
                     /gene="meaB"
                     /locus_tag="ACG0BW_001477"
     CDS             complement(19650..20645)
                     /gene="meaB"
                     /locus_tag="ACG0BW_001477"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFK7258460.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDAIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(20638..22782)
                     /gene="scpA"
                     /locus_tag="ACG0BW_001478"
     CDS             complement(20638..22782)
                     /gene="scpA"
                     /locus_tag="ACG0BW_001478"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFK7258461.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYVESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(22986..23879)
                     /gene="argP"
                     /locus_tag="ACG0BW_001479"
     CDS             complement(22986..23879)
                     /gene="argP"
                     /locus_tag="ACG0BW_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFK7258462.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            complement(23950..25275)
                     /locus_tag="ACG0BW_001480"
     CDS             complement(23950..25275)
                     /locus_tag="ACG0BW_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HFK7258463.1"
                     /translation="MKKANLTGTLVCFVACLIFLSSGDIFREKPIALIWFLRQNILFF
                     TGTLAWCLMTLTMCLSLRSSWLNKVLGGLDKAWRLHKWAGICAIAFAFAHWLDEKLPQ
                     LFVAFGWLTHPGKIVDINLTPVQENWLHAGLLVGECAMFIMIAMIFVSLSKKVPYHLF
                     HLVHRLFPVFYLAIAFHVFTALFKSYWWETPAAYLLILITIPGVFAAFISLLKLNGSK
                     NKHQATIKNIVNHPGQITEVTLELEHAIDYSPGQFAFLTFAHSKESHPFTIASYTKEK
                     NTLRFAIKHLGDYTSTLASSIKIGQSAFVEGPWGKFDFTLPCSHQVWIAGGIGITPFI
                     AQLEYRKHHGASFVPVDLWYCVSRSEDAWYIDKLTSLCAQARVTLHLLDAHKGERLQV
                     HYLTDKIANKGDTHFWFCGPQSFAKALSKGLYENGIASQNFHFDRFCMR"
     gene            complement(25337..25597)
                     /locus_tag="ACG0BW_001481"
     CDS             complement(25337..25597)
                     /locus_tag="ACG0BW_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFK7258464.1"
                     /translation="MSIKQNLFAALFLCTLTGHAFAATEIHKPDNNLEQLSKVSMSTH
                     NSNPAGVESQIKEKAEELQASNYYISSFSGKNYIHATVIFYK"
     gene            complement(25614..26243)
                     /locus_tag="ACG0BW_001482"
     CDS             complement(25614..26243)
                     /locus_tag="ACG0BW_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFK7258465.1"
                     /translation="MKILHIDSSPMLKHSSSRKLSQALVEALCQRFAQATIIHRDVGL
                     TPPPHLREETFLALCGKAECENAQIQQEVNDIHAAIDELASCDVLIIGAPMINHSVSS
                     GLKTWIDQVCQAGLTFRFTSAGAVGLLTDKPTFIVSTRGGIYCDEAHLALDHQESYLQ
                     ATLKLMGITNTHILRAEGVDKTQPGRELAEQNALAQIPDLITHVFNHYL"
     gene            26344..27240
                     /locus_tag="ACG0BW_001483"
     CDS             26344..27240
                     /locus_tag="ACG0BW_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7258466.1"
                     /translation="MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERL
                     GVRLIQRTTRRLALTAAGEQFAVECRALVAQGERARAVVMAAQESPQGMLRVSAPVFM
                     AETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
                     HTQADILVASSQWLARHPQVKAIDDLNRVDGILRLTNNGGSQWQLMNGDQEITLHPEA
                     RLQTNNIRVMLQAVLGGNGVALLPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGR
                     KGMPAIARYFMDELTTRLANGV"
     gene            27296..27955
                     /gene="rpiA"
                     /locus_tag="ACG0BW_001484"
     CDS             27296..27955
                     /gene="rpiA"
                     /locus_tag="ACG0BW_001484"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFK7258467.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28211..29443
                     /gene="serA"
                     /locus_tag="ACG0BW_001485"
     CDS             28211..29443
                     /gene="serA"
                     /locus_tag="ACG0BW_001485"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFK7258468.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            29672..29731
                     /gene="ibsC"
                     /locus_tag="ACG0BW_001486"
     CDS             29672..29731
                     /gene="ibsC"
                     /locus_tag="ACG0BW_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFK7258469.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(29832..30434)
                     /gene="fau"
                     /locus_tag="ACG0BW_001487"
     CDS             complement(29832..30434)
                     /gene="fau"
                     /locus_tag="ACG0BW_001487"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFK7258470.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(30455..30638)
                     /gene="ssrS"
                     /locus_tag="ACG0BW_001488"
     ncRNA           complement(30455..30638)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG0BW_001488"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(30680..31009)
                     /gene="zapA"
                     /locus_tag="ACG0BW_001489"
     CDS             complement(30680..31009)
                     /gene="zapA"
                     /locus_tag="ACG0BW_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFK7258471.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            31177..31755
                     /gene="ygfB"
                     /locus_tag="ACG0BW_001490"
     CDS             31177..31755
                     /gene="ygfB"
                     /locus_tag="ACG0BW_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFK7258472.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            31781..33106
                     /gene="pepP"
                     /locus_tag="ACG0BW_001491"
     CDS             31781..33106
                     /gene="pepP"
                     /locus_tag="ACG0BW_001491"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFK7258473.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            33103..34281
                     /gene="ubiH"
                     /locus_tag="ACG0BW_001492"
                     /gene_synonym="visB"
     CDS             33103..34281
                     /gene="ubiH"
                     /locus_tag="ACG0BW_001492"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFK7258474.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITSVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            34304..35506
                     /gene="ubiI"
                     /locus_tag="ACG0BW_001493"
                     /gene_synonym="visC"
     CDS             34304..35506
                     /gene="ubiI"
                     /locus_tag="ACG0BW_001493"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFK7258475.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAANA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(35580..35639)
                     /locus_tag="ACG0BW_001494"
     CDS             complement(35580..35639)
                     /locus_tag="ACG0BW_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258476.1"
                     /translation="MRGIRKYNAKTEVKLVYFK"
BASE COUNT         8627 a         8783 c         9491 g         8770 t
ORIGIN      
        1 tctcgcgtgc ccaattgctt tcattaaacc gcacgagtat tttattttta ccgccgaacg
       61 gtaccctaaa aataaaataa ggataattta tgcggcttat taattatttt ccagaatcat
      121 caatttcagt tatacattca gcaaaagact ggcaggaagc tatcgatttc tcgatggcat
      181 cattgctgga taaaaactat atcagcgaga attacattca ggctattaaa gattccacca
      241 tgagtaatgg cccttactat attctcgcac caggcgtggc aatgcctcat gcgcgaccag
      301 aatgtggggc gcttaaaacc gggatgtctt taacgttact taaacaaggc gtttattttc
      361 cggggaatga cgaaccaatt aaattactca tcggactctc tgctgccgat gccgattcgc
      421 acattggcgc tattcaggcg ttaagtgaat tactgtgcga agaagaaata ctcgaacaac
      481 tcttaacagc atcatcagaa aaacaattag cggacattat cagccgcgga taattactct
      541 ctcctctaca ggataatatt atggaaaaca agtctgctcg tgcaaaggtc caggcttttg
      601 ggggcttttt gacggcaatg gtcatcccca atattggtgc ttttattgcc tggggtttta
      661 ttactgcgtt atttattccc accggttggc tgcctaacga acatttcgcc aaaattgtcg
      721 gcccgatgat tacctattta ttgcccgtga tgattggttc taccggtggt catctggtcg
      781 gcggtaaacg cggcgcggtc atgggcggaa ttggtactat tggtgtgatc gtcggcgcag
      841 agatcccgat gttccttggc tcaatgatta tggggccgct cggtggtctg gtcataaaat
      901 atgttgataa ggcactggaa aaacgcatac ctgccggttt tgagatggtt atcaataact
      961 tctcattagg tatcgcgggg atgctccttt gtctgctggg ttttgaagtg atcggcccgg
     1021 cggtgttaat tgccaatact ttcgtcaaag agtgtattga ggcgctggta catgcgggtt
     1081 atctgccttt gctgtcagtc atcaatgaac cggcgaaagt gcttttcctt aataatgcga
     1141 tcgatcaggg cgtctattat ccgctgggaa tgcaacaggc atcagttaac ggcaaatcca
     1201 tcttctttat ggtggcctct aatccaggtc ctggcctggg gctgctgctg gcgtttacct
     1261 tgtttggtaa agggatgagt aagcgttctg cgcctggcgc gatgattatt cacttcctcg
     1321 gtgggatcca cgaactgtat ttcccatatg tgctgatgaa gccactgacc attattgcca
     1381 tgattgcggg cggtatgtct ggcacctgga tgtttaactt acttgatggc ggtttggtgg
     1441 ctggcccaag ccctggctct atttttgctt acctggcact gacgccgaaa ggctcgttcc
     1501 tggcgacaat tgccggtgtt acggtaggta ccctggtgtc ctttgctatc acttcgctga
     1561 tactgaagat ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct cagtcagcca
     1621 atgcggttaa ggcgatgaaa caagagggtg cattctcgtt aagcagggtt aagcgtatcg
     1681 cctttgtttg cgatgcgggg atgggctcca gcgcgatggg cgcaaccacc ttccgtaaac
     1741 gtctggaaaa agcggggctg gcaattgaag taaaacatta cgcgatagaa aacgtgcctg
     1801 cggatgcgga tatcgttgtt actcatgcca gtctggaagg gcgcgtgaaa cgtgtgacgg
     1861 ataaaccgct gatactgatt aataactata ttggcgatcc aaaactcgac actttattta
     1921 atcaattaac cgccgaacat aaaaactgat tgccgaggta aaaatgaaaa ccaaagttgc
     1981 tgctatttat ggcaagcggg atgtccgtct gcgcgaattt gaactgccag aaattaccga
     2041 taatgaactg ttagtgagtg taatttctga cagcgtctgt ttatcgacct ggaaagcggc
     2101 gttactcggt agtgaacata aacgcgtacc cgacgattta gaaaatcatc cggtcattac
     2161 cgggcatgaa tgtgccgggg ttattgttga agtgggtaaa aatctcaccg gcaaatataa
     2221 aaaaggccag cgttttgtat tgcaaccggc gatggggtta ccaagcggat attcagcggg
     2281 ctacagctac gaatattttg gcggtaacgc cacctatatg attattcccg agatcgccat
     2341 taacctgggc tgcgtattac cgtaccacgg ctcttatttt gctgcggcgt cgctggcaga
     2401 gcctatgtgc tgcattattg gcgcttatca tgccaattat cacaccacgc aatatgttta
     2461 tgagcatcgc atgggcgtta aacctggcgg caatattgcg ctgctggcgt gtgcaggtcc
     2521 gatgggcatt ggcgctatcg attatgctat taacggcggc atacaaccgt cgcgggtggt
     2581 ggtggtcgat atcgacgaca aacgcctggc gcaggtacaa aagctgctgc cggtggaact
     2641 ggcggccagt aaaggcattg agctggtgta tgtgaatacc aaagggatga gcgatccggt
     2701 ccagacgctg cgggcgctga cggatgatgc cggatttgat gacgtatttg tctatgcagc
     2761 ggtgccttca gtcgtcgaga tggctgatga attactggcg gaagatggct gcctgaactt
     2821 ctttgccggg ccgacggata aaaacttcaa agtgccgttt aatttctaca acgtccatta
     2881 caacagcacg catgtcgtcg gtacgtccgg cggttcaacg gacgacatga aagaggcgat
     2941 tgcgcttagc gccacggggc agttacagcc gtcgtttatg gtgacccata tcggtggcct
     3001 ggatgcagtg ccagaaaccg tgctcaatct gccggatatc cccggaggta aaaaactcat
     3061 ttataacggc gtgaccatgc cgctgactgc cattgccgat tttgccgaaa aaggcaaaac
     3121 cgatccgctg tttaaagaac tggcgcggct ggttgaggaa acgcacggta tctggaatga
     3181 acaggccgag aaatatctgc tggcgcaatt tggcgttgat attggggagg ccgcgcaatg
     3241 atgtccctgg cgtggccatt atttcgcgtt acggaacagg cagcgttggc tgcctggccg
     3301 caaaccggat gtggcgacaa aaataaaatt gatggcctgg cggtcaccgc gatgcgccag
     3361 gcattaaacg acgtcgcttt tcgtgggcga gtggttatcg gcgagggaga gattgaccat
     3421 gcgccgatgc tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc
     3481 gcggttgatc ccattgaagg tacgcggatg gtggcgatgg ggcagagcaa tgcgctggcg
     3541 gtcatggctt tcgcacctcg ggatagcctg ttacatgcgc ccgatatgta tatgaaaaag
     3601 ctggttgtta atcgacaggc tgccggggcg attgatctgt ctttgcctct ggcggacaac
     3661 ttgcgaaacg tggcgaaagc gttaggcaaa ccgctggaca aactgcgcat ggttacgctg
     3721 gataaaccgc gcctgagtac cgcaattgag gaagcaacac agctgggcgt gaaggttttt
     3781 gccctgccgg atggcgatgt cgccgccagc gtgctgacct gctggcagga taatccttac
     3841 gatgtgatgt acaccatcgg cggtgcgccg gagggcgtga tttctgcctg tgccgttaaa
     3901 gcattaggcg gggatatgca ggccgagctg attgactttt gtcaggctaa aggggattac
     3961 acggaaaatc ggcagattgc agagcaggag cgcaagcgtt gcaaggaaat gggcgttgac
     4021 gttaaccgtg tgtactcgct cgatgaactg gtgaggggga acgatatcct ctttagcgct
     4081 acgggcgtga cgggcggcga gttggtgaac ggtatccaac agacggcgaa tggggtgcgg
     4141 acgcagacat tactgatcgg cggcgcggac caaacgtgta atataataga ctccctgcat
     4201 tgatggtgat ctatcggaag accaatggcg acgctgacag aagatgatgt gcttgagcaa
     4261 ctggaggcac aggacaattt attttcattt atgaaaacgg cgcattctat tttgctccag
     4321 gggatacgcc agtttctacc gtcgctgttt gtcgataacg atgaagagat cgtcgaatat
     4381 gcagtgaagc cgttactcgc ccaaagcggc ccgcttgacg atattgatgt tgcactgcgt
     4441 ttgatttatg cgctggggaa aatggataaa tggctgtacg ccgatatcac gcacttttcc
     4501 cagttctggc attacctgaa cgaacagaat gaaatgcccg gatttgccga tgatatgacc
     4561 tgggatttta tcagcaacgt caatagcata acccgcaatg cgacgctcta tgacgcgttg
     4621 aaagcgatga agttcgccga tttcgccgtc tggtcagagg cgcgttttag cgggatggtc
     4681 aaaacggcgc tgacgctggc agtaacgacc accttaaagg aattaacgcc gtgaaaattg
     4741 aattaacggt gaatggtctg aaggtacaag cacagtacca tgttgatgag atagaaaatg
     4801 tacataaacc gttgctgcgc atgttggcgg ccttacagac ggtaaatccg cagcggcgta
     4861 cggtggtttt tctttgcgct ccgccgggga cgggtaaatc taccctaacc accttctggg
     4921 aatatctcgc gcaacaagac ccggaactac ccgctataca gacgctgccg atggatggtt
     4981 ttcaccatta caatagctgg ctggatgcgc atcaattgcg ccccttcaaa ggcgcaccag
     5041 agacattcaa tgtagcgaaa ctggcggaaa acctgcgcca ggtcgtggaa ggggattgta
     5101 cgtggccaca gtacgatcga caaaagcatg atcctgttga agatgcgttg cacgttaccg
     5161 caccactcgt catcgtcgaa ggaaactggt tgttgctgga cgacgagaag tggtgccagc
     5221 ttgcgcaatt ttgtgatttc tcgatcttta tcaacgcgcc agctactgcg ttgcgggaac
     5281 gactggtggg gcgcaagctg gcgggagggt tatcgctggc agacgcagat gccttttatg
     5341 accgtaccga tgggccgaat gttcgccggg tgctggaaga gagtctgcct gcaaatttga
     5401 ccttaatgat gaccgctacg ggtgaatatc atctggtgga ttaacgttcc tgattacacc
     5461 atcttccagt cgcaccaccc gagagaaatg acgccgggta aatgcggcgt catgactgat
     5521 tgctatgacg ctcgtgcctc gctgacggca cttttccagc cagctttcaa acacgctcat
     5581 ccaattttcg tcaaaatccc gactcggctc gtccagcagc aatagcggcg gagagagtgc
     5641 ttccaggcag gcgacggcga ccatccgtcg ctgcgcacta tgcagatcta acggatgcgc
     5701 gctggcagtg tctgctaact gacagcattg cagggcagca tgggtacgtt gagtgatttc
     5761 atccgccggg catttttgca gtttcaaacc aaatgcgatc tcgtcggcga cggtgctgtg
     5821 aaaaagctgg ttttccgctt cctgaaacag tacgccaatc tttgctgcac gttggcggtt
     5881 tttcaggttt ttcatcgcct gttgttgcag cataaccgta ccggctgtgg gcggaagaag
     5941 acctgccatt actcgtaaca gcgtcgattt tcccgcgcca ttgtcgccgg tcagcgccag
     6001 ccattcgcct tgtttgagtt gcagagaaat gtcgcaaaga caatccgttg ccgcgccagg
     6061 ccagcgataa gatatctgat ttaacgttag catagtggct ggagcgctcc gttttgcagt
     6121 tgccacgctt gctggcagta gttcaggaaa ggtgtgcgat gacgttcaaa aagaataatc
     6181 agcgaaccac gttcaaacgc ccagtgttgt aagcgctgca acagcatttc gctggcctgc
     6241 ggcgtcaggc gactaaaggc ttcatcaaga attaatagtt ttggctgcat cgcgatggcg
     6301 caggcgatga ccacgcgctg tgtctctcca ccggagagtg ttgccgggtg acgatgccgc
     6361 aacggctggc actcggtcag agtcagggcg gcatcaatcc gcgccataat ctcttcttca
     6421 gcaagacaca gattttctgg cccaaaggca acttcttctt ccacgctaaa ggtacagccg
     6481 gagagctgca aatagggcga ttgctgaacg agctggatgg tggcggattg ttcattaagc
     6541 gggagatgcc caatgggcgt accgagaagc gtccccgtgc cggttatctc tcctggcaga
     6601 aagtccggat accagcccgc catcagctgt gccagcgtac ttttgccact gccgttgtca
     6661 ccaaagatgg cgaccatccc cgatttgaca tagtgaaagt cataacagaa cggaggatgc
     6721 gttgagtgtg tcgggcaata gctaaactgt tctaacgtaa ccatatccac gctccgaatt
     6781 ctgtcagcat cagcaagatc atggtgtaac gtgctacgcg ttgtaggggg ctatcggctg
     6841 gtgcccataa cgtagtgcgc cggttgtgga tccgaaatgc ccgcatgtcc agcgccgcac
     6901 cacggatagc cagatcgttt agcgcattgt gtgttagcgg aataattagc gcgggcatag
     6961 cacgtaaacg ctgataccag ccctcatcca gcggcacgcc gcgggcgcgc tgcgcttcat
     7021 gaataatcgc taattgccgt tttaactgct caacgaccaa caaaggcccg gcgaacagat
     7081 aagcgacgcc cggcggcagg cgagaagcaa acaaagcgcg gataaaacgt tgcactggga
     7141 cgaattgcat ccacaactgc gaggttgaaa caatcgccag aatccgcaac cagagcgtaa
     7201 tagcatgcgt ccaccgttct ggcgagcgtg gcgtgccgct tagccattct gtcagccagc
     7261 caccgtgtac cagccacagt ccagccccca gcgagaacat cagccagaca acatattttg
     7321 cccgccgccg cgtggccttt agcgcaataa ggcagaaaaa ggttgccgcg ctatagactg
     7381 gcagtattgt ctgtgtgggc aggataagcg tggtgcaggc cgccagcgcc cacagcgtta
     7441 aggatgtaaa cggatgcatt tagcgcacgg cagccattgc cggaaaatgg cgcgtggtgc
     7501 gaatcggcag ctggcgcagt aaaagccaga caatcaccgc tgtaagaatt ttatcgacca
     7561 ggtttgcgcc tatcaccgta atcgccaccg actccaccag gttttgcccc atagagtgca
     7621 tccaggcaac gaacaaatcg gctccgctac cggtgacgcc gccgaacagc gcagtacgca
     7681 gcggtacagc gacgaccgtt actgctagcg taataatcac gccgctgaca acgacttttg
     7741 gcagggtgcg aaaccaaccc gctcgcgcca gccaaccggc gaccagaccg atgaccatcg
     7801 cgacgggcgc aaatgcggcg gcgatcgggt cggtcagcaa accccagagt aaattggtca
     7861 gtaagccggt cagcatcccg atcaccggac caagcagtac cgcgctaata agtgtgccga
     7921 tggagtcgag aaatataggt aatttgacca tgctggccag ttgcccgccg atcatattga
     7981 tggcgataga gataacgatc aacaccaacg cctgactgga aaagtggcga cgcgccataa
     8041 gaaaacctct ctgaaagtag ttatttaacg ggggaaatat gcaattcgtc gtaaccagaa
     8101 agcgcgcaag gttggcggct aaacgccagt tcctcaaggc taccgatcac cagttcaagc
     8161 gtggtccgca cggtacagcc gggcatcata tgaccgccaa gcatcgtgcc gtgtggatcg
     8221 gaaacgcaga gatgaagatg ttcaccactc tgttccagtg tgccgttcaa tgcgatgact
     8281 tcgaacttac cgcttagtag ggccgtgttt tcttgcccgc catagcgtaa ggcgacgtca
     8341 gtcagactgc cggtacaacc tgcaatccac gcagcgtgga gttgttgttg ctgcgcgaag
     8401 gcgcgtaatt gggagagaac ttcctgcccg gggagcaggc gcagcgcgta aaagcgtgcg
     8461 gaggaagaat atgggtaggg caatgtcatc attggagtac tccgaaactc ggacgtattg
     8521 cggataatat cgacgctgca tcagtcatat tctgatataa gtcagggttt gtgacggcac
     8581 aacgaaaaga ttttgctacg cttattcgtg attctgcaaa gtggaataaa ttccacagtg
     8641 gcggggctga aaacgtgacg taagtcagat aattgtcttc tttgactgga caatcattcc
     8701 ttttattcca cgtttcgctt atcctagctg aagcgtttca gtcgattaaa tgttcgacaa
     8761 ttaaccaatc agtcgcagtt tgcgacaggt aaggtttccc cggacgattt gctggattac
     8821 tctgtctggc aaatttgctc agggaaaacc ttgcaggaga tctatgaccg tacgcgtagc
     8881 gataaatggc ttcggtcgca tcgggcgtaa tgtggttcgt gctttgtatg aatccggacg
     8941 ccgggcggaa attaccgtgg tggcaatcaa cgaactggcg gatgctgcgg gcatggcgca
     9001 tttgttgaaa tatgacacca gccatggccg ttttgcatgg gaagtacgac aggaacgcga
     9061 tcaacttttt gttggtgatg acgccatccg cgtattgcat gaacgttcac tgcaatcgct
     9121 cccctggcgt gaacttggcg ttgatgtagt cctcgactgc accggcgtat atggctcccg
     9181 cgagcatggc gaagcacata ttgccgccgg ggctaaaaaa gtgctctttt cacatcctgg
     9241 cagtaacgat ctcgatgcga ccgttgttta cggcgtcaat caggatcaac ttcgtgcgga
     9301 acaccgcatc gtttctaacg cttcctgtac cacgaattgc ataattcccg tcatcaaatt
     9361 gttagatgat gcgtacggta ttgagtccgg cactgtgacc acaattcact ccgccatgca
     9421 cgatcaacag gttattgatg cataccatcc tgacctgcgt cgcacccggg cagccagcca
     9481 gtcgatcatt ccggtcgata ctaaactggc cgccggtatc acacgatttt ttccgcaatt
     9541 taacgatcgc tttgaagcga ttgcggtacg tgtgccaacc ataaatgtga cggcaatcga
     9601 tttaagcgtg acggtgaaga aacctgtaaa agccaatgaa gtcaacctgt tgctgcaaaa
     9661 agcagcacaa ggtgcatttc atggtatagt tgactatacg gaattgccgt tggtctctgt
     9721 agattttaac cacgatccgc acagtgccat tgtcgatggc acccaaaccc gggtcagtgg
     9781 cgcacacctg atcaaaacgt tggtctggtg cgataacgaa tggggctttg ctaaccgaat
     9841 gctcgacacg acgttagcta tggctactgt tgctttcagg taagacgcaa gcagcgtctg
     9901 caaaactttt agaatcaacg agaggattca ccatgtctgt aattaagatg accgatctgg
     9961 atcttgctgg gaaacgtgta tttatccgtg cggatctgaa cgtaccagta aaagacggga
    10021 aagtaaccag cgatgcgcgt atccgtgctt ctctgccgac catcgaactg gccctgaaac
    10081 aaggcgcaaa agtgatggta acttcccacc tgggtcgtcc taccgaaggc gagtacaacg
    10141 aagaattctc tctgctgccg gttgttaact acctgaaaga caaactgtct aacccggttc
    10201 gtctggttaa agattacctc gacggcgttg acgttgctga aggtgaactg gttgttctgg
    10261 aaaacgttcg cttcaacaaa ggcgagaaga aagacgacga aaccctgtcc aaaaaatacg
    10321 ctgcactgtg tgacgtgttc gtaatggacg cattcggtac tgctcaccgc gcgcaggctt
    10381 ctactcacgg tatcggtaaa ttcgctgatg ttgcgtgcgc aggcccgctg ctggcagctg
    10441 aactggacgc gctgggtaaa gcactgaaag aaccagctcg cccgatggtg gctatcgttg
    10501 gtggttctaa agtatctacc aaactgaccg ttctggactc cctgtctaaa atcgctgacc
    10561 agctgattgt tggtggtggt attgctaaca cctttatcgc ggcacaaggc cacgatgtgg
    10621 gtaaatccct gtacgaagct gacctggttg acgaagctaa acgtctgctg accacctgca
    10681 acatcccggt tccgtctgac gttcgcgtag cgactgagtt ctctgaaacc gctccggcta
    10741 ctctgaaatc tgttaacgac gtgaaagctg acgagcagat cctggatatc ggtgatgctt
    10801 ccgctcagga actggctgaa atcctgaaga atgcgaaaac cattctgtgg aacggtccgg
    10861 ttggcgtgtt cgaattcccg aacttccgca aaggtaccga aattgtggct aacgctatcg
    10921 cagacagcga agcgttctcc atcgctggcg gcggcgacac tctggcagca atcgacctgt
    10981 tcggcattgc tgacaaaatc tcctacatct ccactggcgg cggcgcattc ctcgaattcg
    11041 tggaaggtaa agtactgcct gcagtagcga tgctcgaaga gcgcgctaag aagtaaaaaa
    11101 tcacagggca gggaaacctg cccttgtttc agcgcgcttt tagagcacgc attttttcat
    11161 actctaaata attcgagttg caggaaggcg acaagagagt gaatccccag gagcttacat
    11221 aagtaagtga ctggggtgag cgaacgcaga cgcagcacat caacttgaag tatgacgagt
    11281 ataaggcccg acgatacagg acaagagaca tgtctaagat ttttgatttc gtaaaacctg
    11341 gcgtaatcac tggtgatgac gtacagaaag ttttccaggt agcaaaagaa aacaacttcg
    11401 cactgccagc agtaaactgc gtcggtactg actccatcaa cgccgtactg gaaaccgctg
    11461 ctaaagttaa agcaccggtt atcgttcagt tctccaacgg tggtgcttcc tttatcgctg
    11521 gtaaaggcgt gaaatctgac gttccgcagg gggctgctat cctgggcgcg atctctggtg
    11581 cgcatcacgt tcaccagatg gctgaacatt atggtgttcc ggtaatcctg cacactgacc
    11641 actgcgcgaa gaaactgctg ccgtggatcg acggtctgct ggacgcgggt gaaaaacact
    11701 tcgcagctac cggtaagccg ctgttctctt ctcacatgat cgacctgtct gaagaatctc
    11761 tgcaagagaa catcgaaatc tgctctaaat acctggagcg catgtccaaa atcggcatga
    11821 ctctggaaat cgaactgggt tgcaccggtg gtgaagaaga cggcgtggac aacagccaca
    11881 tggacgcttc tgcactgtac acccagccgg aagacgttga ttatgcgtat accgagctga
    11941 gcaaaatcag cccgcgtttc accatcgctg cctccttcgg taacgtacac ggtgtttaca
    12001 agccgggtaa cgttgttctg actccgacca tcctgcgcga ctctcaggaa tatgtttcta
    12061 agaaacacaa cctgccgcac aacagcctga acttcgtatt ccacggtggt tccggttcta
    12121 ctgctcagga aatcaaagac tccgtaagct acggcgtagt gaaaatgaac atcgataccg
    12181 atacccaatg ggcaacctgg gaaggcgttc tgaactacta caaagcaaac gaagcttatc
    12241 tgcagggtca gctgggtaac ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc
    12301 cgcgcgtatg gctgcgtgcc ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc
    12361 aggaactgaa cgcgatcgac gttctgtaag atattccttt ctgcttatct caaggcccgc
    12421 tctgcgggtc ttttttttgc taaaagaaaa attgcctgat gcgctacgct tatcaggcct
    12481 acacgtatcc tgcaatatat tgaattcgca ggtttttgta ggccggataa ggcgttcacg
    12541 ccgcatccgg catgacaaag cgcacattga tatctgtctg agccccgctc tgcggggatt
    12601 ttttcgccaa aagcaaggtg atgcagatga gaaatctgtg atctatttgg caaaattatg
    12661 ctttattgtt tacccttgtc agactgcccg tcataaggcg gcggagtgta tttctccatt
    12721 ttgagtcagt tgaaaaggaa tattgaatgg aagatttgaa tgttgtcgat agcataaacg
    12781 gcgcgggaag ctggctggtg gctaaccagg cgctgctgct aagttatgca gtaaacatcg
    12841 tggcggcact cgcgatcatc atcgttggtt tgattatcgc gcggatgatt tccaacgcgg
    12901 tgaatcgcct gatgatctcc cgtaaaatcg atgccactgt tgctgatttt ctttctgcgt
    12961 tagtccgtta cggaattatc gcctttacgc taatcgctgc actgggccgc gtgggtgtac
    13021 aaacagcgtc agtcattgct gtactcggtg ccgcaggctt agcggttggt ctggctttgc
    13081 aggggtcact gtctaacctg gccgctggcg tgttacttgt catgttccgc ccgttccgtg
    13141 ccggagaata tgttgacctc ggcggcgtag ccggtactgt gctgagtgtg cagattttct
    13201 ccaccaccat gcgtactgca gacggtaaaa ttatcgttat tccgaacggt aaaattattg
    13261 ccggaaatat tattaacttc tcccgcgagc cagttcgccg taacgaattt attattggcg
    13321 tggcgtatga ttccgatatc gatcaggtta agcagatcct gaccaatatt atccagtctg
    13381 aagatcgcat tttgaaagat cgcgaaatga ctgtgcgcct gaatgaactt ggcgcatcgt
    13441 cgattaattt cgtggtccgc gtctggagca acagcggcga tctgcaaaac gtgtactggg
    13501 atgtgctgga gcgtattaaa cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc
    13561 aaatggatgt gaactttaag cgggtgaaag aaaataaaga aaacgaagcg gtaactcgtt
    13621 aatcatgtaa agcgcccatg aagttcatgg gcgcactacc aatttaacgc attgtttcat
    13681 cgagtatatt ggcaactttt tgcatgactt ccccggcttt ctccacgcct tccttatttt
    13741 gcttttctac ccactgtgct tgttcttgct gataaccgat cgctttctgc caggcgtcca
    13801 gccctgattt tttcagatag cgaacctgtg caccttcctg ctcaagcgtt tttaccatgc
    13861 tgatcaggtt gttatctaat gccttgccca gtgctttctc ggttgtaatg gcagctcgtt
    13921 gaatagcctc acgatctctg ttatcaaggt tttcccacga ttgtttattc attaccaaca
    13981 gataaacatg accaagccag agagaaggtg agagcaacac attcggcgca gcacgttcag
    14041 catggatgtc atatccgcta tcgagattga ccattaaacc atccagtttt ccatccatga
    14101 gtgctttagt tatttgatca ttccacggta aagttacggt ttttgccccc gtatgagtta
    14161 aataagcccg atgccagaaa ctggctgttc gccaggtggt tccctgaagc gcagtcaatt
    14221 tatcaatggg cctggtagag aaaaagccta ccgggtagcc aaggaaaaac tgtaaattca
    14281 cgatattgtt acgctcaagt tcagcgttaa attcgggaat ttccgcatat acgcgacgaa
    14341 agaattctac ctgactggct ccatgatccg ggccaatagc aaagctcttg aagatttgat
    14401 gaagcggcaa ttgtttcgcg gtgtattccg gcaccactat acccatatca gctttgctgc
    14461 catcgcttat tgtcgtcaga gcgtcatagc tgatggcggt ttcaccgttc cagtgtggtt
    14521 cgattttcaa acgcccctgc gactcttttt caatttctgc aaaaaatatc tctttaatta
    14581 tttgtgtacg cattcccccg tagggttcat ggtctgtata acgaagaatt ttttcagcag
    14641 caaaaatcga gggagataac agaagaaatc ctgataaaag aaaggggagt aattgtcggt
    14701 agtgcatatc tgttccttca gtttattaat tcaacttata ctatattaat tttatcaatt
    14761 atccctaatg ttattcctgc gctatagtct gcgcatcaga taattagccc ggaattaatt
    14821 acgtgatatc ttattacttt caaggtcttg cgcttggcgc agctatgatc ctaccgctcg
    14881 gtccacaaaa tgcttttgtg atgaatcagg gcattcgtcg tcagtaccac attatgattg
    14941 ccttactttg cgctatcagc gatttggtcc tgatttgcgc cgggattttt ggtggcagcg
    15001 cgttattgat gcagtcgccg tggttgctgg cgctggtcac ctggggcggc gtagccttct
    15061 tgctgtggta tggttttggc gcttttaaaa cagcgatgag cagtaatatt gagttagcca
    15121 gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc caccatgctg gcagtgacct
    15181 ggctgaatcc gcatgtttac ctggatactt ttgttgtact gggcagcctt ggcgggcaac
    15241 ttgatgtgga accaaaacgt tggtttgcgc tcgggactat tagcgcctct ttcctgtggt
    15301 tctttggtct ggctattctc gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac
    15361 aacgcattat caatctggtt gtgggatgtg ttatgtggtt tattgccttg cagctggcga
    15421 gagacggtat tgctcatgca caagccttgt tcagttaggc gctatctgat ggaaaaataa
    15481 aacagaggcg ctaagcttgc ctccagaggt cctgaatttt aggcaagtca attaacacgg
    15541 agagactaac gtgaagttca aagttatcgc cctggcggca ttaatgggta ttagcgggat
    15601 ggcagcgcag gctaacgaat tgccggatgg accgcatatt gtcacctccg gtacggcaag
    15661 cgtggatgcg gtgccagaca ttgccactct tgcgattgaa gtcaacgtgg ccgcgaagga
    15721 tgccgctact gccaagaaac aggcggatga gcgcgtcgca caatacattt ccttccttga
    15781 actcaatcag atcgcgaaaa aagatatcag ctcagcgaac ttacgcaccc agccagatta
    15841 tgattatcag gatggtaaaa gtatcctgaa aggctaccgt gctgtgagaa cggtggaagt
    15901 cacgctccgt cagttagaca aactgaattc cttgctggat ggcgcgctga aggcgggtct
    15961 taacgaaatt cgttctgtgt cgctgggcgt ggcgcagccg gatgcctata aagacaaagc
    16021 gcgtaaggca gcgattgata acgcgattca tcaggcgcag gaactggcga acggctttca
    16081 tcgtaaactg gggccggtat atagcgtgcg ctaccatgtt tccaactatc agcccagccc
    16141 aatggtgcgg atgatgaaag ccgatgccgc gccggtgtcc gcccaggaaa cttacgagca
    16201 ggccgctatt cagtttgatg atcaggtcga tgtggtcttc cagttagaac ctgtggatca
    16261 acaacccgct aaaacacctg cagcacaata atatttgtag cccgacgtat tcgtcgggct
    16321 aatgccttta cccgatattg cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat
    16381 cacctatctt tgctcgcggt ttcatcaagg ataggctgac tgaaacggtt aagcggacaa
    16441 ggatgcgggg gagggggtat ggacattttt atttcgaaaa agatgcgcaa tttcatttta
    16501 ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca
    16561 tttggtaaaa gcattgccgc gctggaagaa caaattggct atacgctatt tacccgcaaa
    16621 gataataata tcagcctcaa taaagccgga caggaactgt atcaaaaact gttcccggtt
    16681 tatcaacgac tttctgcgat tgataatgaa atccataact cagggcgtcg ttcacgagaa
    16741 attgtgattg gcatagataa tacttatccg acaattattt tcgatcagtt gattagtctc
    16801 ggagataagt atgaaggcgt gaccgcccag ccagttgagt tcagcgaaaa cggcgttatc
    16861 gataatcttt ttgaccgcca gttagatttt attatctccc cacagcatgt atctgcacga
    16921 gttcaggaac tggaaaacct gactatcagt gaactaccgc cgctgcgatt gggctttctc
    16981 gtttctcgcc gttatgaaga gcgtcaagag caagagctgc ttcaggagct gccctggcta
    17041 caaatgcgct ttcagaaccg ggccaacttc gaagcaatga ttgatgcgaa tatgcgacct
    17101 tgtgggatca atcccaccat tatttatcgt ccctacagtt ttatggccaa aatcagcgcc
    17161 gtagagcgcg gacattttct gacggtgatc ccgcattttg cctggcgact ggtgaatccg
    17221 gcaacgctga aatattttga tgcgccgcac aggccgatgt atatgcagga atatctttat
    17281 tcaatcagaa atcatcgtta taccgccacg atgcttcagc atattgctga agatcgtgac
    17341 gggacgagtc attaacccag catcgagccg gttgcaatta aattacggtg taaatcgaag
    17401 acgtggctaa gatcgtggtg aatatgtccg ccgggcgcat tttccagata gcgatgcaga
    17461 taatcccgat acataggatg tgcacaatta tcaatgatgg tgcgggcgcg ttgaagcggg
    17521 gaaagaccac gcagatcggc gatcccttgt tcggtgatga tcactttgac gctgtgttcg
    17581 ctgtgatcaa catggctgca cattggcacg acggttgaga tcttgccttc tttagcaatc
    17641 gacggggcca taaagatcga cagatacgcg ttgcgttcaa aatcaccgct gccaccgatg
    17701 ccgttcatca gatcgacccc ggctacgtgc gttgagttgg catgcccgta aatatcaaac
    17761 tccaggccga cgttcagagc gatgacgccc agacgacgga tgatttccgg gttattggaa
    17821 atctcctgcg gacgcaacac aatgcggctg gcaaagtaat ccatattgtc gtaaatcttg
    17881 cgcagggaat cggccgagat agtcaggctg gaggcgctgg ccccgctgat tttgcccgtt
    17941 tccagtaaat gcaccaccga ttcctgtagc acttccgaat acatcatgaa cggaggaatt
    18001 tccgggtttt cccctagtcg cgccatgact gcattattga tattgcccac gccactttgc
    18061 agcggcagaa attccggcgg aatacgccca tgcgccattt cctgcaacaa gaacgtgacc
    18121 acgttatcgg caatctgctg gcacatggga ttttgcttat ccagcatatt accggcgtcg
    18181 ggcaagttgg tttccacgac ggcgacaatc tttttcggat cgatttgcac atagcgggta
    18241 ccgacgcgat ccattgcatg gaagatcgac acgctattgc gccgcggtgg cgcgccagga
    18301 atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc
    18361 actttcttcg cccgcagcag ccatgtcggc gcattaccga tcccgctggt taaccagact
    18421 cgaccatccg gtgccagtgc cgatgcttca atgacggcaa catcaatgtc gccgaagaaa
    18481 ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt caacgaaact caccgcgccc
    18541 tgattgatct ttttacgtaa accggacgat gtttgatatg gcgcacgcca ggaaacagca
    18601 tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg acgcccccgt cagcaggcga
    18661 atttgatacg gctttttggc ctcatgctgt tcgttagctc tgcgggcaat cgcggtgggt
    18721 aaggctttcg gcgaacccgc tggggtaaag ccgctaaatg ccaccatgtc gttatgctgg
    18781 ataatttctg ccgcttcatc ggcggtcatc cttgtccact gagtttccat tttaactcct
    18841 tcgttcgcag ggattaatga ccaacgaaat taggtttacg tttttcgagg aaagcgttca
    18901 tcccttcctg gtagtcttcg ctgtcataaa ccgcgcggcg catcccctga atacgttcaa
    18961 attcatcgga gttcatggtg tgcgcttcgc ccagtacacg cagctcttct ttgataacgg
    19021 caatggctaa cggcgctttc tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca
    19081 gttcttccac ttccacaaca tggttgagga tgccgacagc cagcgcgcgc tgggctgtga
    19141 ttggcgaagc ggtaaaaatc agctctttga caatgtggaa gcccgcgtcg cgggtcaggt
    19201 tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac aggtgtcatt gagaaggttg
    19261 aggtactggc ggcgatgatc agatcggaac tcatgatcat ttcaaatgcg ccaccccaga
    19321 cactaccttc caccatcgaa atgatcggtt tcgggaattt ttggatcatg cgggtaattt
    19381 gacgcaacgg atcatcatag gagagcggat cgcgaccgcc agacggcagt tcgtgaatat
    19441 cgtgacctgc ggagaagact ttggatccac tcggcgcgcg caaaatgata cagcgaattt
    19501 ccggccggtt gagatcgcta aacgcctgca taagatcatc aataaagact ttacttaagg
    19561 cattaagttt tcggccatag ttaaactcaa tgaccgccac tttgttgata gtgacaacgt
    19621 tcacatactg ataagacata aaaattcctt taatcaaaat attgcgtctg gataaattca
    19681 ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc
    19741 tggcggtaat agcgatcgaa atcttcattc gcgaacaggt gattcagtac ttcttcttcg
    19801 gtttgtttac gcagccattc caccgattgt tgttgccgca cttgttgtaa acgaccactg
    19861 gcagttagcg cggttttgaa gtcgataatg gcgtgccaga tcgcatcgat tccacgtttt
    19921 tccagtgcgc tacaagtcag aacccgtggc tgccattcgt cgtatttacg tcgcagaata
    19981 tgcagggcac tctcgtacat atgccgggca atggcgacat tggtatggtt atcgccatcg
    20041 tctttgttga taacgatcag atctgccact tccatcagcc cctttttaat gccctgcaga
    20101 tcatcgccgc caccggcaat ttgcaacgag ataaaacagt ccaccatgcg ggcgacttcc
    20161 gtttctgact gcccgacgcc aaccgtttcg acaatcacta cgtcataacc cgccgcttcg
    20221 cataacagca ttaattcccg cgctcgctga ctggcaccgc ccagatggcc ggaggagggt
    20281 accgggcgaa taaacgccgc ctcggcacgc gccaggtcat tcatgcgggt tttatccccg
    20341 agaatgctac cgccagtgac cgggctgctg ggatcgactg caataaccgc aacctttaat
    20401 ccctcacgaa tcaacaacat gccaaaggct tcaagaaagg tacttttccc cgcgccgggt
    20461 gtgccggtaa cgcccagtcg cagggcgtta ccacagaacg gcataatggc atcaagcagc
    20521 tgcgtactta gcgcctgatg acgcgggtga cggctttcta ccagcgtcat ggcctgggcg
    20581 agcgtggcac gctcaccctg acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta
    20641 atcatgatgc tggcttatca gattcagtac gtcgcgcacg ctgtcgagca taggcgtacc
    20701 tggaccataa atcgccgcca cgccgcgctc ttgcaggaag gcgtaatcct gcggcggaat
    20761 gacgccaccg gcgactacgc agatatcttt gcgtccccat tttttcagcg cttcgaccag
    20821 ttccgggatc agcgttttat gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc
    20881 gttttctact gccaggcggg cgatctcttc aggtgtagag aacatcgggc ttaaatctac
    20941 gtcgaaaccg agatcggaat aggcgctggc gatcactttc gcgccgcgat cgtgcccatc
    21001 ctggcccatt ttagcgatca aaatgcgcgg gcgacgacca ttgtcggcaa ggaactgctc
    21061 cgtttgcgca acaatggcat cgaactcgga ggccgatttc tcagactgat gatagctttg
    21121 cgcaatcacg ccggtaacac actggcttgg caccagataa cggtcgaaag cggcttccag
    21181 cgcatcggaa atttcaccca gggtggcgcg aacgcgagca gcattaacag cggcagccag
    21241 caggttttcg ttatgctgtg cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc
    21301 atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt tgctcgttac gcaccatcac
    21361 gttgtcgatc tcaaggacgt cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc
    21421 aacgatgaca cgcttgccct ggtcgatcag cgactgttcg cgcgctgagg cctcttcgat
    21481 cattcgtttt ggcagacctg cttcgatcgc tttcgccatg ccaccggctt cgtcgatctg
    21541 ttggataata gctctggctt gtttgacgat ttgatcggtc agcgactcaa cgtaatagga
    21601 tccggccagt ggatcgacgg tgcggcagag ttctgattct tcctgaatga tgatctgggt
    21661 attgcgggca atgcgtgctg agaaatcggt aggcaaacca agcgcttcat caaaggcgtt
    21721 ggtatgcagt gactgagtac cgcccagcgt tgcagccagt gcttcaatgg tggtgcggat
    21781 aacgttgtta tacggatcct gttcagtcag gctccagcct gaggtctggc agtgggtacg
    21841 cagcgccagt gatttcggat cctgtgcgcc aaatccactg accgcttcgc tccataaata
    21901 acgtgccgca cgcaacatgg cgacgttcat aaacagatcc atgccaatgc cgaagaagaa
    21961 cgacaggcga ggagcgaagt catcaatttt cagcccggcg gagattgctg ctttgatgta
    22021 ctcaatccca tcagcgagcg taaatgctac ctgctgcacg cagttggcac ccgcttcacc
    22081 catgtggtaa ccgctgatac tgatggtatt aaatcgcggc atgttgccgg aacaccaggc
    22141 gatgatgtct gcgataatac gcattgacgg ttttggcggg taaatatagg tgttgcggca
    22201 aagatactct ttgaggatat cgttctgaat ggtgccggtc agtttatcag gtgtaacacc
    22261 ttgctcttcc gcggcgacga tataaaacgc cagtactggt agcactgcgc cattcatggt
    22321 catcgaaacc gacattttat ccagtgggat ctggtcgaac aggactttca tatcttccac
    22381 ggtgtcgata gcgacgcccg ctttgccgac gtcgcccgcc acgcgtgggt tatcggagtc
    22441 gtagccacgg tgggtggcaa ggtcaaacgc aacggaaaga cctttttgcc cggcggccag
    22501 gttacggcga taaaaagcgt tggactcttt tgctgttgaa aaaccagcat actgacggat
    22561 ggtccacggt tgggcggtat acatagtggc acgcgggcca cgaacgtagg gcggcaaacc
    22621 aggaagggta cctgtcacct ccagattatc gagatcggct tcggtataca gcggcttgat
    22681 ggcgatccct tccgcggttt gctgaaccag cgagtcgaca gttttctccc gacggctcaa
    22741 ttccttgttg gcaagctgtt gccactcctg cacgttagac attatttgct ccgtaaaacg
    22801 caatcaaaat gagggtattt gcgcctacgg tgagcttttt acgtgggttt gtcgccagca
    22861 aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt tgtgatgtga
    22921 ttcaaggaat gcaaaaagac gtgcggataa cgcctgatat gtgctatcag gcattatttg
    22981 atggattaat cctgacgaag gactttgtga ccataatcga gtaacgcatc agtgacttta
    23041 cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccgtcg ttgaaatagc
    23101 ccaggcgtta agtcaatcag ttcaccgctg gccagctctt tctcgatttg caggtgcggg
    23161 atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga agaattaacg
    23221 atatggcagg gtacgctgcc aggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc
    23281 atatcgtcaa gatggtcaaa ggcgaccact ggcgctttca ataatgccga acgcgttacg
    23341 ccgttaggga aatatttttc ggcaaacggt tttgagctga cgaacagata gtcgagcgca
    23401 ccaagtttat cgacaagaca actcggcagt gcctgatgtt gaatactcac cgcgccgacc
    23461 acttcgccgc ggcgcagacg ttcctgagtg cgggtttcat cttctacctg caagttgagg
    23521 cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt cgccagactg
    23581 tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc atcgcccagc
    23641 cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg cccttgttcc
    23701 gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt ttccagttgc
    23761 ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc gcgctcaaat
    23821 cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt
    23881 gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt tgcgttgggt
    23941 acagagcatt taacgcatac aaaaacggtc gaaatggaag ttttggctgg caatcccatt
    24001 ttcatataac cctttgctga gcgctttggc aaaactctgt gggccgcaaa accagaaatg
    24061 ggtgtcacct ttatttgcta ttttatcagt aagatagtgc acctgtaggc gctcaccttt
    24121 atgcgcatca agcagatgca gtgtgaccct ggcctgggca caaagtgagg tcagtttatc
    24181 aatataccag gcatcctcgc ttcggctgac gcaataccag agatccacag gaacaaagga
    24241 cgcgccatga tgtttacgat actcaagctg ggcgataaag ggggtaatgc cgatgcctcc
    24301 tgctatccag acctggtggg agcaagggag agtaaaatca aactttcccc atggcccttc
    24361 aacgaaggcg gactggccga tttttatcga ggatgccaga gtcgaggtat aatcccctaa
    24421 atgtttgatc gcaaagcgca acgtgttctt ctctttggta tatgaagcga tagtaaaagg
    24481 gtgagattct ttactgtgtg cgaacgttaa gaatgcaaac tgtcccggag aatagtcgat
    24541 agcatgttcc agctcaaggg tgacttcggt aatttgtccc ggatggttaa caatgttttt
    24601 aatggtagcc tgatgcttgt ttttactacc gtttaatttc agcagcgaaa tgaaagccgc
    24661 aaagacgccc ggaatagtaa ttaaaattaa taaatacgca gcaggagttt cccaccagta
    24721 tgatttaaac aaagcagtaa acacatgaaa ggcaattgcc agataaaaga cggggaacaa
    24781 acggtgaaca agatgaaata aatgataggg aactttttta ctaagagaca cgaaaatcat
    24841 cgctatcata ataaacattg cacattcacc caccaacagt cccgcatgaa gccaattttc
    24901 ttgtactggc gtcaggttaa tatcaacgat tttaccaggg tgagtaagcc agccaaaggc
    24961 gacgaataac tggggtaatt tttcatccag ccagtgggcg aaagcaaacg ctatggcgca
    25021 tattcctgcc cacttatgta atcgccatgc tttatccaga ccgcccagca ctttgttaag
    25081 ccatgatgaa cgcaacgata aacacatcgt cagcgtcatc agacaccacg caagggttcc
    25141 cgtaaaaaag agtatgtttt gtcgaaggaa ccatattagc gcgattggct tttcacgaaa
    25201 aatatcaccg gacgacaaaa aaataaggca tgcgacaaag caaaccaacg tacctgtaag
    25261 attagctttt ttcatttttt atttcatttt ggcagcggag catggaataa ttccatgctc
    25321 ccgttaaaca gagtgattat ttatagaaaa tcacagtggc gtggatataa tttttaccgg
    25381 agaaagaact gatgtagtaa ttgctggctt gtaactcttc cgctttctct ttgatttggc
    25441 tttcaacgcc cgctggattg ctattgtgtg tggacatgct cacttttgat aactgttcaa
    25501 gattattgtc cggtttgtgt atctcggttg ctgcaaatgc gtgtcctgtg agagtgcaaa
    25561 gaaataatgc ggcaaataaa ttttgcttaa tagacataat aactcctttt atattaaaga
    25621 taatgattaa atacgtgtgt tatgagatca gggatttgcg ctaatgcatt ttgctcggcg
    25681 agttcgcgtc ccggttgggt tttgtcgacg ccttccgcac gtaaaatatg tgtattggta
    25741 atgcccatta atttcagcgt ggcttgcaga taactttcct gatgatccag cgccagatgt
    25801 gcttcatcgc aatagatacc gccgcgcgtc gagacaataa acgtcggttt atccgtcagt
    25861 aacccaacgg caccagcgga agtaaaacga aacgttaaac cagcctgaca gacctgatct
    25921 atccaggttt tgaggccaga tgaaacgctg tgattaatca tcggcgcgcc aataatgagc
    25981 acatcgcacg atgccagttc atcaatagca gcgtggatat cattcacctc ctgctgaatt
    26041 tgtgcgttct cgcactccgc tttaccgcaa agcgccagaa aggtttcttc acgcaaatgg
    26101 ggtgggggcg tcaggccaac gtcgcgatga ataattgtgg cttgcgcgaa acgttggcaa
    26161 agcgcttcta ccagcgcctg gcttaatttc cgcgaacttg aatgcttaag cattggactg
    26221 ctatcgatat ggagaatttt catggggttg ctctcatttc tgcgatgaga gaagtttaat
    26281 tgttgttgat atggtgatta aatagacata attgaacttc attgttgtga aaatagaaca
    26341 atcatgatcg actggaatga ttactactac tttgccctga ttgccgatga aggcagctac
    26401 agccaggcgg cgctgcgtgc gggtgtgtcg aaatcggtat taagccgtcg aatggcagcg
    26461 ctggaagagc gcctcggcgt gaggcttatt cagcgaacta cgcggcgtct ggccctgacg
    26521 gcggcagggg aacagtttgc tgtcgagtgt cgggcgctgg tggctcaggg ggaacgggcc
    26581 agagcggtgg tgatggccgc ccaggaaagc ccacagggaa tgctgcgcgt cagcgcgccg
    26641 gtatttatgg ctgaaacatg gtttggtgag ttcgtcagtg aatttatcgc ccgctggccc
    26701 ttggcgaagg tgcaactgct ggcaattaat cgcccggtgg atatggtcgc tgaaggcatt
    26761 gatattgcgc tggttacggc ttccggcgcg cttgcggact cgacgctgca ttataaaaaa
    26821 ttacataccc aggctgatat tttagttgcc agttcgcagt ggctggcccg ccatccgcag
    26881 gtgaaggcca ttgacgatct taaccgcgtc gacgggatcc tccgcctgac gaacaatggc
    26941 gggagccagt ggcagttaat gaatggcgac caggagatta cgctgcatcc cgaggcgcgt
    27001 ttgcagacaa ataacattcg cgtcatgttg caggcagtgc ttggcgggaa tggcgtggcg
    27061 ttattgcctc aatttgcctg taaacagggg cttgccaacg gtgaactggt gcgcctgttc
    27121 gcaccgtgga gcggcatacc cagaccgttg tatgctttat ttgccgggcg aaaggggatg
    27181 cccgccattg cgcgatattt tatggatgag ttaaccacgc ggcttgccaa cggggtctga
    27241 atcgcttttt ttgtatataa tgcgtgtgaa atttcatacc acaggcgaaa cgatcatgac
    27301 gcaggatgaa ttgaaaaaag cagtaggatg ggcggcactt cagtatgttc agcccggcac
    27361 cattgttggt gtaggtacag gttccaccgc cgcacacttt attgacgcgc tcggcacgat
    27421 gaaaggccag attgaagggg ccgtttccag ttcagatgct tccactgaaa aactgaaaag
    27481 cctcggcatt cacgtttttg atctcaacga agtcgacagc cttggcatct acgttgatgg
    27541 cgcagatgaa atcaacggcc acatgcaaat gatcaaaggc ggcggtgcgg cgctgacccg
    27601 tgaaaaaatc attgcttcgg ttgcggaaaa atttatctgt attgccgacg cctccaagca
    27661 ggttgatatt ctgggcaaat tcccgctgcc agtagaagtt atcccgatgg cacgtagtgc
    27721 agtggcgcgc cagctggtga aactgggcgg tcgtccggaa taccgccagg gcgtggtgac
    27781 cgataacggc aacgtgatcc tcgacgtcca cggcatggaa atccttgacc cgatagcgat
    27841 ggaaaacgcc ataaatgcga ttcctggcgt ggtgactgtt ggcttgtttg ctaaccgtgg
    27901 cgcggacgtt gcgctgattg gcacacctga cggtgtcaaa accattgtga aatgatctga
    27961 cgggggaacc tcccccgtta aaaaaattct cttcattaaa tttggtgaca tgtgtcacgt
    28021 ttttacctgg ctattgtcga ttgctctaaa taaaccctct aaaccagcat attcatccaa
    28081 gaaatacctt tgcgtgatat ttcctcaaca ttgcgacgca aacgttcata ttgccgcaat
    28141 attatttttt gatatgttga aaggcggatg caaatccgca cacaacattt caaaagacag
    28201 gattgggtaa atggcaaagg tatcgctgga gaaagacaag attaagtttc tgctggtaga
    28261 aggcgtgcac caaaaggcgc ttgaaagcct tcgtgcagcg ggttacacca acatcgaatt
    28321 tcacaaaggc gcgctggatg atgaacaatt aaaagaatcc atccgcgatg cccacttcat
    28381 cggcctgcga tcccgtaccc atctgactga agacgtgatc aacgccgcag aaaaactggt
    28441 cgctattggc tgtttctgta tcggaacaaa ccaggttgat ctggatgcgg cggcaaaacg
    28501 cgggatcccg gtatttaacg caccgttctc aaatacgcgc tctgttgcgg agctggtgat
    28561 tggcgaactg ctgctgctat tgcgcggcgt gccggaagcc aatgctaaag cgcaccgtgg
    28621 cgtgtggaac aaactggcgg cgggttcttt tgaagcgcgc ggcaaaaagc tgggtatcat
    28681 cggctacggt catattggta cgcaattggg cattctggct gaatcgctgg gaatgtatgt
    28741 ttacttttat gatattgaaa acaaactgcc gctgggcaac gccactcagg tacagcatct
    28801 ttctgacctg ctgaatatga gcgatgtggt gagtctgcat gtaccagaga atccgtccac
    28861 caaaaatatg atgggtgcga aagagatttc actaatgaag cccggctcgc tgctgattaa
    28921 tgcttcgcgc ggtactgtgg tggatattcc ggcgctgtgt gacgcgctgg cgagcaaaca
    28981 tctggcgggg gcggcaatcg acgtattccc gacggaaccg gcaaccaata gcgatccatt
    29041 tacctctccg ctgtgtgaat tcgacaacgt ccttctgacg ccacacattg gcggttcgac
    29101 tcaggaagcg caggagaata tcggcctgga agttgcgggt aaattgatca agtattctga
    29161 caatggctca acgctctctg cggtgaactt cccggaagtc tcgctgccac tgcacggtgg
    29221 gcgtcgtctg atgcacatcc acgaaaaccg tccgggcgtg ctaactgcgc tgaacaaaat
    29281 cttcgccgag cagggcgtca acatcgccgc gcaatatcta caaacttccg cccagatggg
    29341 ttatgtggtt attgatattg aagccgacga agacgttgcc gaaaaagcgc tgcaggcaat
    29401 gaaagctatt ccgggtacca ttcgcgcccg tctgctgtac taattcccct tctctgaaaa
    29461 tcaacgggca ggtcactgac ttgcccgttt ttttatccct tctccacact gttgccgcgt
    29521 tgcgcattct actttgcgag tctgctccag aaaataaatc aaagtcagat caatgcgttg
    29581 tgtttattta atggggattt acgatggcag gacagcacag ggctgtaacg gtaaagccct
    29641 caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgtcatca tactgattgt
    29701 actgttactc ataagtttcc cagcttatta acagtcagtc tcaggggagg agtcatcctc
    29761 ccttaccctt actcactaaa ttaggtcaaa gaatcaacga tgtcaatcag ggcgatgcag
    29821 gtgtgtcgcc ctcaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata
    29881 tcccactctt caacggggag tttttccacc aactgacaat catgcgcata acccaccggt
    29941 tgcattttat agtgctgcca gttttgtaag gttcgatcat aaaaaccacc gcccattccc
    30001 aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcaacacgtc taatcgggaa
    30061 aggggtagca cgtcacgcac atccagtttt ggctcatgga tcttcaacct gttcatcacc
    30121 agttcacttt gcggatggta attcaggaac agcaaattac cggcactaaa gggatgcaaa
    30181 actggaagat atacgcgctt accggcgcgc cagagttgtt ctatgagtgg ctgggtgtcg
    30241 agttcgccat caaaagagag gaatacagcg accgtatgcg ccatcaccac tgggggatac
    30301 gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg cgttaacgta
    30361 cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagttct
    30421 ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc
    30481 tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg
    30541 acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc
    30601 tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac
    30661 cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt
    30721 gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg
    30781 ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata ttcaatgcgg
    30841 caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc
    30901 gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca
    30961 cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct
    31021 gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata
    31081 gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca
    31141 tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct
    31201 ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac cccagctgag
    31261 atgcatggtt taatcagcgg gatgatatgt ggcggtaacg atgacagctc atggctgccg
    31321 ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcactg
    31381 cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt
    31441 tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcact ggcaggttgg
    31501 gttaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggacaa agtgaccgga
    31561 gaaaccggtg aagccatcga cgatctgcgt aacatcgcgc agttgggcta cgacgaagac
    31621 gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgagtacgt ccgtgttgcc
    31681 gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa
    31741 ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca
    31801 agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct
    31861 gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa
    31921 cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg tgctgattaa
    31981 aagcgatgac actcataacc acagcgtttt gtttaaccgc gttcgcgacc tgacggcaga
    32041 gatctggttt ggtcgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg
    32101 cgcactggca ttcagtgaaa tcaatcagca actttatcaa ctacttaacg gcctggatgt
    32161 ggtttaccat gctcagggcg aatatgcata tgctgatgaa atcgtgaaca gtgcgctgga
    32221 aaaactgcgt aaaggctcgc gacaaaatct caccgcgcca gcaacgatga tcgactggcg
    32281 tcccattgtt catgaaatgc gcctgttcaa atcgccggaa gagattgccg tacttcgccg
    32341 cgcgggtgaa atcaccgccc tggcgcatac ccgggcgatg gaaaaatgcc gtccgggaat
    32401 gttcgagtac cacctggaag gcgaaattca ccacgaattt aaccgccacg gcgcgcgcta
    32461 tccttcctat aacaccattg tcggcagcgg tgaaaacggt tgcattctgc actataccga
    32521 aaacgagagt gaactgcgtg acggtgacct ggtgttgatt gatgcgggtt gcgaatacaa
    32581 aggttacgct ggcgatatta cccgcacctt cccggtcaac ggcaaattca ctcaggccca
    32641 gcgtgaaatc tacgacattg tgctggagtc tcttgaaacc agcctgcgcc tgtatcgtcc
    32701 gggaacttcc atgcaggaag tcactggtga agtggtgcgc atcatggtta gcggcctggt
    32761 aaaactcggc atcctgaaag gtgatgttga tgaactgatc gctcagaacg cccatcgtcc
    32821 tttctttatg catggcctta gccactggtt aggactggat gtccatgacg ttggcgttta
    32881 tggtcaggat cgctcgcgca ttctggagcc ggggatggta ctgaccgtag agcctggtct
    32941 gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc ggtatcggca ttcgtattga
    33001 agacgacatt gtgattaccg aaaccggcaa cgaaaacctc accgccagcg tggtgaaaaa
    33061 gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc
    33121 ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg
    33181 ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat
    33241 ggacgagcta ttgcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtctgg
    33301 caatctctgg ctgattgcgc aaccgccatc accagcgtgc atgtcagcga tcgtggtcat
    33361 gccggattcg tcaccctcgc cgcagaagat taccaactgg cggcgctggg gcaggttgtc
    33421 gaattgcata atgtcgggca acggctgttt gcattgctgc gcaaagcacc tggcgtaacg
    33481 ctgcattgtc ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt tgaagtgacg
    33541 ctggagagtg gcgagacgct gacgggccgc gtgctggtag cagctgatgg cacccattca
    33601 gcgttagcca ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca actggccgtg
    33661 attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg cttcacgaaa
    33721 catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt ctggtgtcat
    33781 ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc
    33841 cagtctgcct ttggctggcg actggggcaa attacccacg ccggtaaacg cagtgcttat
    33901 ccgctggcgt taacccgcgc cgccagacct attacccatc gtaccgtgct ggtgggcaat
    33961 gcggcgcaaa ctctgcaccc gattgccggg caagggttca acctcggtat gcgagatgtc
    34021 atgagccttg cggaaactct gactcaggcg caggagcgcg gagaagacat gggtgattac
    34081 ggcgtattgt gccgttatca gcagcgtcga cagcgcgatc gcgaagcaac catcggcgtc
    34141 acggacagcc ttgtacatct ttttgccaac cgttggaccc cgctggttgt cgggcgcaac
    34201 atcgggctga tgacgatgga attatttacc ccggcacgcg atgtgctggc gcagcgcacc
    34261 ctcggttggg tggcgcgttg aaggtggttt taaggaataa acaatgcaaa gtgttgatgt
    34321 agccattgtt ggcggcggca tggtggggct ggcggttgcc tgcggcttac aggggagcgg
    34381 cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcga atgcaccacc
    34441 acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt
    34501 ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag tgtgggacaa
    34561 agacagcttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc atcttgggca
    34621 tatcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga
    34681 tatcactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa atgaaacctt
    34741 cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg cggacggcgc
    34801 taattcctgg ttgcgcaaca aagccgatat tccgctgact ttctgggatt atcagcatca
    34861 cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc ggcaggtttt
    34921 ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcgattgt
    34981 ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag acgaatttaa
    35041 tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgtaaggttg agagtgagcg
    35101 ccttgtgttc ccactgacgg ggcgttatgc gcgccaattt gccgcgcacc gtctggcgtt
    35161 ggtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggggtaa atctcggctt
    35221 tatggatgct gcagagctgg ttgctgaact gaaacggttg catcgtcagg ggaaagacat
    35281 cgggcagtac atttatctgc gtcgctatga gcgtagccgc aagcacagtg cggcgctgat
    35341 gctggctggt atgcagggat ttcgcgatct gttttccggt accaatccgg cgaaaaaact
    35401 gctgcgtgat attggtttaa aactggccga cacgcttcct ggcgttaaac cacaacttat
    35461 ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcttctg
    35521 ctgtttattt gatacccatc acactttcat ctcccggttt tttcgacggg agattttcct
    35581 catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat gccacgaatt
    35641 tttgatttag tgttttttca cattttttca g
//
LOCUS       DAYPHN010000031        35516 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000031 DAYPHN010000000
VERSION     DAYPHN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.031"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(42..1901)
                     /gene="gss"
                     /locus_tag="ACG0BW_001495"
     CDS             complement(42..1901)
                     /gene="gss"
                     /locus_tag="ACG0BW_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFK7258477.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHLAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2106..2972
                     /gene="yghU"
                     /locus_tag="ACG0BW_001496"
     CDS             2106..2972
                     /gene="yghU"
                     /locus_tag="ACG0BW_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFK7258478.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3001..3082)
                     /locus_tag="ACG0BW_001497"
                     /pseudo
     CDS             complement(3001..3082)
                     /locus_tag="ACG0BW_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3095..3343)
                     /gene="hybG"
                     /locus_tag="ACG0BW_001498"
     CDS             complement(3095..3343)
                     /gene="hybG"
                     /locus_tag="ACG0BW_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFK7258479.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3356..3697)
                     /gene="hypA"
                     /locus_tag="ACG0BW_001499"
     CDS             complement(3356..3697)
                     /gene="hypA"
                     /locus_tag="ACG0BW_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFK7258480.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3690..4178)
                     /gene="hybE"
                     /locus_tag="ACG0BW_001500"
     CDS             complement(3690..4178)
                     /gene="hybE"
                     /locus_tag="ACG0BW_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFK7258481.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4171..4665)
                     /gene="hybD"
                     /locus_tag="ACG0BW_001501"
     CDS             complement(4171..4665)
                     /gene="hybD"
                     /locus_tag="ACG0BW_001501"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFK7258482.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4665..6368)
                     /gene="hybC"
                     /locus_tag="ACG0BW_001502"
     CDS             complement(4665..6368)
                     /gene="hybC"
                     /locus_tag="ACG0BW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFK7258483.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6365..7543)
                     /gene="hybB"
                     /locus_tag="ACG0BW_001503"
     CDS             complement(6365..7543)
                     /gene="hybB"
                     /locus_tag="ACG0BW_001503"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFK7258484.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7533..8519)
                     /gene="hybA"
                     /locus_tag="ACG0BW_001504"
     CDS             complement(7533..8519)
                     /gene="hybA"
                     /locus_tag="ACG0BW_001504"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFK7258485.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8522..9640)
                     /gene="hybO"
                     /locus_tag="ACG0BW_001505"
     CDS             complement(8522..9640)
                     /gene="hybO"
                     /locus_tag="ACG0BW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFK7258486.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9829..10116)
                     /gene="yghW"
                     /locus_tag="ACG0BW_001506"
     CDS             complement(9829..10116)
                     /gene="yghW"
                     /locus_tag="ACG0BW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFK7258487.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10235..11119)
                     /gene="yghX"
                     /locus_tag="ACG0BW_001507"
                     /pseudo
     CDS             complement(10235..11119)
                     /gene="yghX"
                     /locus_tag="ACG0BW_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
     gene            11276..12316
                     /gene="gpr"
                     /locus_tag="ACG0BW_001508"
     CDS             11276..12316
                     /gene="gpr"
                     /locus_tag="ACG0BW_001508"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFK7258488.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12356..12850)
                     /gene="yqhA"
                     /locus_tag="ACG0BW_001509"
     CDS             complement(12356..12850)
                     /gene="yqhA"
                     /locus_tag="ACG0BW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFK7258489.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13041..13925
                     /gene="yghA"
                     /locus_tag="ACG0BW_001510"
     CDS             13041..13925
                     /gene="yghA"
                     /locus_tag="ACG0BW_001510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFK7258490.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14197..14622)
                     /gene="exbD"
                     /locus_tag="ACG0BW_001511"
     CDS             complement(14197..14622)
                     /gene="exbD"
                     /locus_tag="ACG0BW_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFK7258491.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14629..15363)
                     /gene="exbB"
                     /locus_tag="ACG0BW_001512"
     CDS             complement(14629..15363)
                     /gene="exbB"
                     /locus_tag="ACG0BW_001512"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFK7258492.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15615..16802
                     /gene="metC"
                     /locus_tag="ACG0BW_001513"
     CDS             15615..16802
                     /gene="metC"
                     /locus_tag="ACG0BW_001513"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFK7258493.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16942..17601
                     /gene="yghB"
                     /locus_tag="ACG0BW_001514"
     CDS             16942..17601
                     /gene="yghB"
                     /locus_tag="ACG0BW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFK7258494.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17641..18540)
                     /gene="yqhC"
                     /locus_tag="ACG0BW_001515"
     CDS             complement(17641..18540)
                     /gene="yqhC"
                     /locus_tag="ACG0BW_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFK7258495.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18734..19897
                     /gene="yqhD"
                     /locus_tag="ACG0BW_001516"
     CDS             18734..19897
                     /gene="yqhD"
                     /locus_tag="ACG0BW_001516"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFK7258496.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20002..20829
                     /gene="dkgA"
                     /locus_tag="ACG0BW_001517"
     CDS             20002..20829
                     /gene="dkgA"
                     /locus_tag="ACG0BW_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFK7258497.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21029..21955
                     /gene="yqhG"
                     /locus_tag="ACG0BW_001518"
     CDS             21029..21955
                     /gene="yqhG"
                     /locus_tag="ACG0BW_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFK7258498.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATASY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22006..22263
                     /gene="yqhH"
                     /locus_tag="ACG0BW_001519"
     CDS             22006..22263
                     /gene="yqhH"
                     /locus_tag="ACG0BW_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFK7258499.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22306..24525)
                     /gene="ygiQ"
                     /locus_tag="ACG0BW_001520"
     CDS             complement(22306..24525)
                     /gene="ygiQ"
                     /locus_tag="ACG0BW_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFK7258500.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24636..26048)
                     /gene="ftsP"
                     /locus_tag="ACG0BW_001521"
     CDS             complement(24636..26048)
                     /gene="ftsP"
                     /locus_tag="ACG0BW_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFK7258501.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26123..26860)
                     /gene="plsC"
                     /locus_tag="ACG0BW_001522"
                     /gene_synonym="parF"
     CDS             complement(26123..26860)
                     /gene="plsC"
                     /locus_tag="ACG0BW_001522"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFK7258502.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27094..29352)
                     /gene="parC"
                     /locus_tag="ACG0BW_001523"
     CDS             complement(27094..29352)
                     /gene="parC"
                     /locus_tag="ACG0BW_001523"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFK7258503.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29490..31097)
                     /gene="ygiS"
                     /locus_tag="ACG0BW_001524"
     CDS             complement(29490..31097)
                     /gene="ygiS"
                     /locus_tag="ACG0BW_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFK7258504.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31230..31625)
                     /gene="mqsA"
                     /locus_tag="ACG0BW_001525"
     CDS             complement(31230..31625)
                     /gene="mqsA"
                     /locus_tag="ACG0BW_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HFK7258505.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31627..31923)
                     /gene="mqsR"
                     /locus_tag="ACG0BW_001526"
     CDS             complement(31627..31923)
                     /gene="mqsR"
                     /locus_tag="ACG0BW_001526"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HFK7258506.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32128..32610)
                     /gene="ygiV"
                     /locus_tag="ACG0BW_001527"
     CDS             complement(32128..32610)
                     /gene="ygiV"
                     /locus_tag="ACG0BW_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFK7258507.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32663..33055)
                     /gene="ygiW"
                     /locus_tag="ACG0BW_001528"
     CDS             complement(32663..33055)
                     /gene="ygiW"
                     /locus_tag="ACG0BW_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFK7258508.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33207..33866
                     /gene="qseB"
                     /locus_tag="ACG0BW_001529"
     CDS             33207..33866
                     /gene="qseB"
                     /locus_tag="ACG0BW_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFK7258509.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33863..35212
                     /gene="qseC"
                     /locus_tag="ACG0BW_001530"
     CDS             33863..35212
                     /gene="qseC"
                     /locus_tag="ACG0BW_001530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFK7258510.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35258..>35516)
                     /locus_tag="ACG0BW_001531"
     CDS             complement(35258..>35516)
                     /locus_tag="ACG0BW_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFK7258511.1"
                     /translation="FFAFNKYDWMLESGGNICSIPSVSGEDRILQAMIAAFFLLTPLI
                     ILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         8738 a         9453 c         8921 g         8404 t
ORIGIN      
        1 gactattctt cataatcagt acgggattgc tacaacatgt attactcttt caccacaatt
       61 aacggttcaa tatcactctc ttttttaata accagtgatt catcaccgcg caaacacgtc
      121 ccaccgtagt tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc
      181 ggcaaacacc acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg
      241 tccagcacct cttcatgatg gctgaccagg tcgatattgc tgccacagcg accggcaatc
      301 ggtttcactg cgtaacctgt tttcaccagt tcatcattaa cagtgaaatc ggtatccagc
      361 aggtaacgat ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg
      421 atcaccgtcc acagcggctc aaagaccagc acttccgggc gcagcaatac gtcgataaga
      481 cgcacttcgt tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta
      541 acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc
      601 agccgtcctt ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg
      661 cgcaagatac gcgtttcaaa gcccgccagg tgcagcgcct gctccataaa ctgcgcgtga
      721 tagttttcct cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt
      781 ttccaggcac cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta
      841 tagccctgct ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag
      901 tcggcgttgt attcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca
      961 gtgatcatat ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc
     1021 gggatgtcga acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga
     1081 tacatcaggt gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg
     1141 gtgtagtaat gatacggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt
     1201 tgtagcggat ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccaggcgc
     1261 gctccgctga ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta
     1321 tcttccgtct ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttcagggta
     1381 tagcagccgt tttcgaccac catctccagc tcacgcgtcc actgttgccc ttgcggcaac
     1441 ggggaatgaa tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg
     1501 atggcgacat ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc
     1561 gcgaccggcg cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta
     1621 accacttctc gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag
     1681 accacaccgt aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg
     1741 cccatatatt cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga
     1801 tcgagagaac tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat
     1861 gtcccgaacg gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata
     1921 caacctaagc agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg
     1981 acagaggtca gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt
     2041 cacattctgt acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac
     2101 tcactatgac agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg
     2161 gcggcgcgtt cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc
     2221 cggtgggcaa acatccatta caactttatt cgctgggaac gccgaacggt cagaaagtaa
     2281 cgattatgct tgaggagctg ctggcgctgg gcgttactgg tgcagagtac gacgcctggc
     2341 tgattcgtat tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact
     2401 cgaagatccc ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg
     2461 gttcgatcct gctttatctg gcggagaaat ttggctactt cctgccgcag gatttggcaa
     2521 agcgtactga aacgatgaac tggctattct ggttacaggg cgcggcaccg ttcctcggcg
     2581 gtggttttgg tcacttttac cattacgcac cggtaaagat tgagtacgcc atcaaccgct
     2641 ttaccatgga agccaaacgt ctgctcgacg tgctggataa gcaactggcg cagcataagt
     2701 ttgttgcggg cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaacg
     2761 tggtgttagg tggtgtgtat gatgccgctg agtttcttga tgcgggcagt tataagcatg
     2821 tacaacgctg ggcgaaagaa gtaggcgaac gtccggcggt gaaacgtggg cgtattgtta
     2881 accgcaccaa cggaccgctg aatgagcagt tgcatgagcg ccatgacgcc agtgatttcg
     2941 agacgaatac ggaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc
     3001 caggccggat aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc
     3061 tgccgcgtct tatcaggcct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca
     3121 tttggcgcag tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa
     3181 atccgacgtg taccagcacc cactggccca gtagatcggc agggttacct tcacaaatca
     3241 gggcgatatt cacatcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat
     3301 cttcaccgac agccagcacc tggcctggaa cgccaataca cattattaac tccggttatt
     3361 caacttcaat acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccgt
     3421 gacagagcgg acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc
     3481 accaggcctg ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt
     3541 ggcagacaat ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa
     3601 tttccagcca cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa
     3661 tttcaacggc gctctggcaa agagacaact catgcatttt cgccactcct gcgaccaaac
     3721 agcagggcgc gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc
     3781 attcgtgcgc agtcatccgt caggcgttgg ccctcttcaa tcgacatgct gtgcgaaagc
     3841 ggcgacatca gcgaacagga gagatattgc gaaacaccgt ccagttcgcc aacggtaaag
     3901 gtcatagtgc catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag
     3961 agttgatccg ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc
     4021 gtccactgac cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga
     4081 aaagagagat cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc
     4141 ttcggggagg tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc
     4201 ttccacgcct gattcgcgca gcgcagccag aacctgctca agcgcaggtt caatcattgc
     4261 ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca cgccgaccag
     4321 ggtcagcttt ttcggaaact caccggtgaa gcgcagggcc gacaatacgt cggccaggcc
     4381 aagctgatgc ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat
     4441 catcatcgtt cccggcgtgt tctttttcga cacaatggca tccgcaataa tcagatgatc
     4501 gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac
     4561 ataatccggc agaatgtatc gttgctctaa cgcttcgaca atccgcacac cgatggcttc
     4621 atcggtcagc aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact
     4681 gaaaccactt cgttgccgtc ggcatccact acgtgtaccg cacaggccat gcacgggtcg
     4741 aaggagtgaa tggtacgcac cacttccagc ggtttattcg gatcagcaac cggtgtaccc
     4801 accagcgact gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag
     4861 gttgatggaa caactgcctg gtagttactg ataataccgt ctttaataac catccagtga
     4921 gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga
     4981 atgttcggtt tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg
     5041 tattgatttt gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc aataatacgg
     5101 cccagcgtgg agtgcagctg cgccacttcc agcgtgttgc cagtcagttt ctgataaatc
     5161 gcaacgattt cattcagttt gttctgggta gattcgcgac ctgccgccag tttcaccagc
     5221 atattagcca gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag
     5281 gaatatttcc cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt
     5341 gcctgcggcg cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc
     5401 aggtattcat cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg
     5461 tagccgcccg ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc cggcacgctc
     5521 aggtagttca ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcaataact
     5581 gcggtatcaa ccttataaac ctgttcaaca aagtcgctca gcttgtcgat gaaagacttg
     5641 atgtacatca ggcgctcaag gttcagcacg cccaaaccgt cgaggttgat cgggttcgcg
     5701 acaccaccta ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg
     5761 cggttagcgt cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc
     5821 acttccggcg gcagtttcat tgccgggtga ccccagtagc cgttggcgaa aatccccaac
     5881 tgaccgctgg caaccagatc tttgatcttg ttctgaactt tggtgaactc ttccgggctg
     5941 ttcagatgcc aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc
     6001 agtgcagaag tgatatccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg
     6061 tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg
     6121 tcgatattca gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat
     6181 acgccacaga tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc
     6241 tcttccatgc cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg
     6301 atttcgcaat cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc
     6361 tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta
     6421 tcggcagtag acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag
     6481 aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca
     6541 ccagcgaata ggtcagacgc caggttgcac aacctaacag tgcgctcagt gctgacagga
     6601 acagcatgcg ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca
     6661 tcagcaggac ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca
     6721 gcttgtcgcg atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac
     6781 tgatggtgtt ggtcagcttg acgaacagac tcttttcatc cggaccgttg ccacgcagac
     6841 ccgcctgcac cagcgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca
     6901 gcagcgagaa cagcggcaac atttcatagc tctgccacaa cggatgcacc ttgtagcccg
     6961 ccgagatcat cagtgacccc attgaagact ggtgcatggt cggcaacagc gcgccaagtg
     7021 caatgatgaa gaacatcacc ttgttcagac gcttgagaga aaccttccag cccagacgtt
     7081 caaacagtgc cggagcaaac tccagtgcca tcacgccgat atagatggtc atacaaaccg
     7141 ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt
     7201 tccagtagcg acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac
     7261 tcgccaacag cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg
     7321 cccacgccag cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga
     7381 tccacacgcc ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca
     7441 gacgcttcac aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt
     7501 tactgatgat tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg
     7561 tgatggtcgt ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt
     7621 ggtagcatca tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa
     7681 agatcgtcca gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc
     7741 tgagtaccgc cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc
     7801 aggtaagtgt cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc
     7861 agcgccagac gttttttcgc ctccgccatc agctcttcac gcgtaccgaa aatcaccgcg
     7921 cccgccgggc acacttctac gcagccaggt agaccgcctt tatcgagacg ttccacacct
     7981 ttctggttgc acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc
     8041 ggcacgttgt acggacaggc gaccatgcag taacggcagc cggtgcacac gtctttgtcg
     8101 taatggacaa tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca
     8161 cagttcggat cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc
     8221 tggtctttgt tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc
     8281 gacagtttgt cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaaattg
     8341 atatcctgac acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac
     8401 atccccagcg atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc
     8461 agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg
     8521 gttattctcc ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg
     8581 ccatcacgct gacaccggca accaacccaa ccacaccgcc gagcaaacca atagcgcctg
     8641 cagagacgtt gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtttgat
     8701 tttcgacgtt ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc
     8761 catagcacgg gtgaccaatc gccaccggcc acacaccgcc aacatcgcag aattgcagcg
     8821 ttgagcagtt gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt
     8881 cgcggtggcc ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac
     8941 gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca
     9001 gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt
     9061 tcggcgggca gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga
     9121 ctgcgccagt tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga
     9181 tagcgataat ggctgctgcg ccttccgccg ctttgcggat gtgatccaca atcggctcac
     9241 cggcaaccat gcaataaata ccgttatctt ttaatgggat ggaaccatcc accactaaca
     9301 catactgccc tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc tgatgaccga
     9361 aggcggcgga aagcacttcg tgatactcca gagagatagt ctccagtacg aggttttcta
     9421 ccgttggatg cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa
     9481 tccagataac tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt
     9541 tgctacttaa ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt
     9601 taatgccgtg agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga
     9661 agacctggca tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta
     9721 ccacacattt attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg
     9781 aagaagcatt agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag
     9841 gcgataaccg gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc
     9901 cattaccatc tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct
     9961 aagctggcga tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg
    10021 tttataatcg gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa
    10081 tcccgccatt aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatt
    10141 gtcatatata gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct
    10201 tgtttaagac gtaatcccgt aagagataaa cctatcagga gagatattta tcgaaccatt
    10261 tcagtgtcct ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat
    10321 tatggaatcc gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt
    10381 tcaacgccgc ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt
    10441 ggagtagtaa aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa
    10501 acggcaccgc gcaggccagt tccggatacg cgacagccgc cgcgttcgat acgccaccgc
    10561 catagcaaaa tccggtaata cccactttgc ctgtcgcttg cggatagcgt tgcataaact
    10621 caattgcggc aaagaaatca ttcatcagtt tgttggatca acctgctgtt gcagctcacg
    10681 acctttatca tcatttcccg gataacctcc aacggaactt aagccgtcag gtgccagggc
    10741 gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg
    10801 attctcatgc accaccacta cggctggcgt tttgccgctc atctttgcgg gcttcaccag
    10861 ataaccccgc acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat
    10921 ttccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa
    10981 caacgctaat gccgtcatcc cgcctactgc acttcgccgc aaggttgagg aactcacgtt
    11041 tcgagatttt cccgtgagcg taatagtcat agtaatccag caactcttgt gggaaatctt
    11101 tggcggttaa acgcggcatc gttgcactcc tcagttggtg tttttttaag caaagcataa
    11161 gcacgtgttt ttgcccagtt tttcgtcact ctgtgagcca gactacggga tacgcgctgg
    11221 cgaatcgcta aactagaaac attgtttcga aattgaacgg tggaaaggag aggtcatggt
    11281 ctggttagcg aatcccgaac gttacgggca gatgcaatac cgctattgcg gaaaaagtgg
    11341 tttacgcctg cccgcgttat cgctcggttt atggcacaat ttcggtcacg ttaacgcgct
    11401 ggaatcacag cgtgcgatcc tgcgtaaagc gtttgatttg ggcattacgc actttgattt
    11461 agccaacaat tacgggccgc ctccaggaag cgcagaagag aactttggtc gcctgctgcg
    11521 ggaggatttt gccgcttatc gcgatgaact gattatctct accaaggctg gctacgatat
    11581 gtggcccggc ccttacggct ctggcggttc acgtaaatac ctgctcgcca gcctcgacca
    11641 aagcctgaag cgtatggggc ttgagtatgt cgatatcttt tactctcatc gcgtcgatga
    11701 aaatacgccg atggaagaaa ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc
    11761 gctgtatgtc gggatctcct cttactcgcc agagcggacg caaaaaatgg tcgagttgct
    11821 gcgcgagtgg aaaattccgc tgttaattca tcaaccttcg tacaatttac tgaaccgctg
    11881 ggtggataaa agcggcctgc tggatactct gcaaaataac ggcgtgggct gtattgcctt
    11941 tactcctctg gctcagggat tgctgaccgg aaaatatctc aacggcattc cgcaagattc
    12001 acggatgcat cgtgaaggga ataaagttcg tggtctgaca ccgaaaatgc ttaccgaagc
    12061 caacctcaac agcctgcgct tattgaatga aatggcacag cagcgtggac aatcaatggc
    12121 gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc
    12181 cagccgcgcg gagcaactag aggagaacgt gcaggcgctg aataatctga catttagcac
    12241 caaggagctg gcgcagattg atcagcatat cgccgatggc gagctgaatc tgtggcaggc
    12301 gtcttccgat aaatgacctg ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt
    12361 gattatgacg agtcagtcgg tcaagatagc ccatcacaaa tgcagaaagc acaaacgtca
    12421 gatggataat gacgtaccac atcagtttgt tatccgggac atttttcgca tccataaaga
    12481 cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc tgctactttg tttttcagcg
    12541 acgttgcgtc cattttcccc agccagttca gcttctcttt gttctcggag atatccagct
    12601 gtgagacgaa attctcataa ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg
    12661 tcatatccac cagcgacagc aacacgagga tcaaatctga ttccgccatc gagaagatat
    12721 tcggcagtac gtgaataatc tcctggaaga acttcagcgc cagggcaact aacgccagcg
    12781 aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc atacattgca ttttcaagaa
    12841 aacgttccat acattcctgt cgtgtagtta aaacgggcaa cagtatatcg caattgagta
    12901 aacagcacgc aacagcaaat caacatatac gttaaacctt tgtcgcataa ttctgaggtt
    12961 tagggatctt cctggttccc ttatcaacgc atcgagaacg tctaccctta aaggacgttg
    13021 aacctgaagg gagaaaaacg atgtctcatt taaaagaccc gaccacgcag tattacactg
    13081 gtgaatatcc caaacagaaa caaccgacgc caggcatcca ggcgaagatg acaccggtac
    13141 cggattgcgg cgagaaaacc tatgttggta gcggtcgcct gaaagatcgt aaagcactgg
    13201 tgacaggggg cgattccgga ataggtcgcg ctgccgccat cgcttacgcg cgtgaagggg
    13261 ctgacgtggc gatcagttat cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga
    13321 tcattgaaga atgcggacgc aaagccgttc tgctgccagg cgatttaagc gatgagaaat
    13381 ttgcccgttc gctggttcac gaagcgcaca aggcgttagg cgggctggat attatggcgc
    13441 tggtcgccgg gaaacaggtt gccattccgg atattgcaga cctcaccagc gaacagtttc
    13501 aaaagacctt tgccattaac gttttcgcgc tgttctggct aacccaggaa gcgatccccc
    13561 tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat ccaggcatac cagccaagtc
    13621 cgcatttact ggactatgcg gctacgaagg cggcgattct gaactacagc cgtggcttgg
    13681 caaaacaggt cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc ccgatctgga
    13741 cagcactgca aatttccggc ggacaaacgc aggataagat cccgcagttt ggtcagcaaa
    13801 cgccgatgaa acgtgcgggg caaccggcgg aactggcccc tgtatatgtt tatctggcaa
    13861 gtcaggagtc gagctacgtc accgcagaag tgcacggcgt gtgcggcggc gagcatttag
    13921 gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca ttgtgaattc actcttacct
    13981 gatgtggcga aaacgcctta tccgcctaca aacgcatgaa gattcaacat gttgcaatag
    14041 attttgtagg cctgataagc gtagcgcatc aggcattgag cacctaatgc cggatgcgtc
    14101 gtgaacgcct tatccggcct acaaacgcac gaagattcaa catgttgcaa tagattttgt
    14161 aggcctgatg agcgtagcgc atcaggcaat tctactttac ttcgctttgg cggtttcttc
    14221 gccgaccaga cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa
    14281 cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc
    14341 ggttaacgca ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa
    14401 catcgagttg tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt
    14461 gctggtagaa gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat
    14521 aaagataatc agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat
    14581 ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc atcggatatt atcctgcgcg
    14641 taatttttgt gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg
    14701 gctttgcagc aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat
    14761 ctggcgtgca aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc
    14821 cgttgctaac agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt
    14881 ttgcgcgata ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa
    14941 cggagaaatc gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac
    15001 tgcggcgacc cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc
    15061 gctgccttct gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct
    15121 tttgctacca aaatcagcgg cgatatcgtt ggcctggttt aaggaacgcg cttcagccag
    15181 cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc tcttactgaa
    15241 gaagattgcc caggtgacta cggaggccaa aataagccca atcatcacgc acttaacgac
    15301 aatatcggcg tgctgataca taccccagac ggaaaggtcc gtctgcatta aattattacc
    15361 cactgtgtat ctccaggacg caagtcacaa aatctgcgca taataatatc aaaacgacgt
    15421 cgaattgata gtcgttctca ttactatttg catactgccg tacctttgct ttcttttcct
    15481 tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc
    15541 caattcttca ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa
    15601 acaggaatcc cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca
    15661 gcaaaaaata cactctcggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtct
    15721 ttgacagtgt agaagccaaa aaacacgcga cacgtaatcg cgccaatgga gagttgttct
    15781 atggacggcg cggaacgtta acccatttct ccttacaaca agcgatgtgt gaactggaag
    15841 gtggcgcagg ctgcgtgcta tttccctgcg gggcggcagc ggttgctaat tccattcttg
    15901 cttttatcga acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg
    15961 atttctgtag caaaatcctc agcaaactgg gcgtaacgac atcatggttt gatccgctga
    16021 ttggtgccga tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc
    16081 caggctccat caccatggaa gtccacgacg ttccggcgat tgttgccgcc gtacgcagtg
    16141 tggtgccgga tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg
    16201 cgctggattt tggcatcgat gtttctattc aagccgccac caaatatctg gttgggcatt
    16261 cagatgcgat gattggcact gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa
    16321 atgcctatct gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc
    16381 tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggctgaat
    16441 ggctggcaga acatccgcaa gttgcgcgag ttaaccaccc tgctctgcct ggcagtaaag
    16501 gtcacgaatt ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta
    16561 agaaaaaact caataatgaa gagctggcga actatctgga taacttcagt ttattcagca
    16621 tggcctactc gtggggcggg tatgaatcgt tgatcctggc aaatcaacca gaacatatcg
    16681 ccgccattcg cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg
    16741 gtctggaaga tgtcgacgat ctgattgccg atctggacgc cggttttgcg cgaattgtat
    16801 aacattgcca cttttggaca attttgcaga cattttattg tgaaaagtct taaattgttg
    16861 cgtccgggat caaggcgtcc tggacgattc aggagtacaa taggcagata aaggcttaaa
    16921 cgctgttcca caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa
    16981 cacgactttg ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt
    17041 gccacgctgt ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg
    17101 ttgatactgg caggcgcatt gattgcccag ggggttatgg attttctgcc tacgattgcg
    17161 attctgaccg ccgcagcaag tctgggctgc tggctaagtt atattcaggg gcgctggtta
    17221 gggaatacca aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc
    17281 gccacctgca tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca
    17341 tttgtccgta cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc
    17401 cagtttttca actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat
    17461 gccttaagta tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg
    17521 atcctgccaa ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa
    17581 aaaaaatact gtaacgcctg acgattttcc ccgttcccgg ttgctgtacc gggaacgtat
    17641 ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta
    17701 acgtttaaac tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgctgctgc
    17761 gctggccttc atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt
    17821 caaatactgc aacggcgagg tactggtgac agacttaaaa ttatggtgga acgccgatac
    17881 gctcatgttg gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc
    17941 aatccgtttc agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag
    18001 taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt
    18061 gccgagaata cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca
    18121 taaaatctct tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa
    18181 atgctcatct tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag
    18241 acgcaaccct gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg
    18301 caccgtcagc agcaggtatt cattggcatc ataacgaaac acgcgttcat tgatataacc
    18361 gattttatgc ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtacgtgc
    18421 gaaaggcgtc tcgccataca acaaacgcac atcgggcaac agttctgaca aactattttc
    18481 tttatttttc agtttattaa ctttatccgc cagcaagcgg caaatctctt cacgtttcat
    18541 atcgcgtaat ttcttaggaa taatgcggca atttgattgt gcgcaatttt gtagcatttc
    18601 tccagcactc tggagaaata ggcaagacat tggcagaaat gagcattgag agccagggcg
    18661 ctggcgatca caatgaaaaa catcaggcag atcgttctct gccctcatat tggcccagca
    18721 aagggagcaa gtaatgaaca actttaatct gcacacccca acccgcattc tgtttggtaa
    18781 aggcgcaatc gctggtttac gcgaacaaat tcctcacgat gctcgcgtat tgattaccta
    18841 cggcggcggc agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg
    18901 catggacgtg ctggaatttg gcggtattga gccaaacccg gcttatgaaa cgctgatgaa
    18961 cgccgtgaaa ctggttcgcg aacagaaagt gactttcctg ctggcggttg gcggcggttc
    19021 tgtactggac ggcaccaaat ttatcgccgc agcggctaac tatccggaaa atatcgatcc
    19081 gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga tgggctgtgt
    19141 gctgacgctg ccagcaaccg gttcagaatc caacgcaggc gcggtgatct cccgtaaaac
    19201 cacaggcgac aagcaggcgt tccattctgc ccatgttcag ccggtatttg ccgtgctcga
    19261 tccggtttat acctacaccc tgccgccgcg tcaggtggct aacggcgtag tggacgcctt
    19321 tgtacacacc gtggaacagt atgttaccaa accggttgat gccaaaattc aggaccgttt
    19381 cgcagaaggc attttgctga cgctaatcga agatggtccg aaagccctga aagagccaga
    19441 aaactacgat gtgcgcgcca acgtcatgtg ggcggcgact caggcgctga acggtttgat
    19501 tggcgctggc gtaccgcagg actgggcaac gcatatgctg ggccacgaac tgactgcgat
    19561 gcacggtctg gatcacgcgc aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa
    19621 acgcgatacc aagcgcgcta agctgctgca atatgctgaa cgcgtctgga acatcactga
    19681 aggttccgat gatgagcgta ttgacgccgc gattgccgca acccgcaatt tctttgagca
    19741 attaggcgtg ccgacccacc tctccgacta cggtctggac ggcagctcca tcccggcttt
    19801 gctgaaaaaa ctggaagagc acggcatgac ccaactgggc gaaaatcatg acattacgtt
    19861 ggatgtcagc cgccgtatat acgaagccgc ccgctaagct ttttacgcct caaactttcg
    19921 ttttcgggca tttcgtccag acttaagttc acaacacctc accggagcct gctccggtga
    19981 gttcatataa aggaggaacg tatggctaat ccaaccgtta ttaagctaca ggatggcaat
    20041 gtcatgcccc agctgggact gggcgtctgg caagcaagta atgaggaagt aatcaccgcc
    20101 attcaaaaag cgttagaagt gggttatcgc tcgattgata ccgccgcggc ctacaagaac
    20161 gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca acagagaaga actgttcatc
    20221 accactaagc tgtggaacga cgaccacaag cgcccccgcg aagccctgct cgacagcctg
    20281 aaaaaactcc agcttgatta tatcgacctc tacttaatgc actggcccgt tcccgctatc
    20341 gaccattatg tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa
    20401 agcatcggcg tgtgcaactt ccagatccat cacctgcaac gcctgattga tgaaactggc
    20461 gtgacgcctg tgataaacca gatcgaactt catccgctga tgcaacaacg ccagctacac
    20521 gcctggaacg cgacacacaa aatccagacc gaatcctgga gcccattagc gcaaggaggg
    20581 aaaggcgttt tcgatcagaa agtcattcgc gatctggcag ataaatacgg caaaaccccg
    20641 gcgcagattg ttatccgctg gcatctggat agcggcctgg tggtgatccc gaaatcggtc
    20701 acaccttcac gtattgccga aaactttgat gtctgggatt tccgtctcga caaagacgaa
    20761 ctcggcgaaa ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc
    20821 ggcggctaac atgcaaattc tcccggtggc ggtaatgttc cgctaccgga cttttcagaa
    20881 atcatttatt cccctcgcgt cccgcccgtt gttactcttc cttgttcagg aatgccaaat
    20941 ataaggacat catcatgcag agccggaagc tcttaaaaga acaactcatc tatatccggg
    21001 ataaacgcaa cggagaggtg aaaaacagat gaaaataata cttctgtttt tagcagccct
    21061 ggcaagtttt accgtacacg cacagccccc ctcacagacc gtagaacaaa cagtccggca
    21121 tatttatcag aactataaat cagatgccac tgcctcttat tttggtgaaa ccggagagcg
    21181 ggcgataact tctgcgcgta ttcaacaggc gcttaccctg aacgacaatc ttacgctgcc
    21241 gggcaatatt ggctggctgg attatgatcc ggtttgtgat tgtcaggatt ttggcgatct
    21301 ggtgctagaa agcgtcgcga taacccaaac tgacgccgat catgccgatg ccgttgtgcg
    21361 ctttcgtatc tttaaagatg ataaagaaaa gaccacgcag acactgaaaa tggtggcgga
    21421 aaatggtcgt tgggtcattg acgatattgt cagcaatcat ggcagcgtct tacaagcagt
    21481 taatagcgag aatgaaaaaa cgctggccgc tttagcttcg ttgcaaaaag aacagccgga
    21541 agcctttgtt gccgaactct ttgaacatat tgctgattat agctggccgt ggacgtgggt
    21601 ggtttccgac tcttaccgcc aggcggttaa tgccttctat aaaaccacct tcaagacggc
    21661 caataatccc gatgaagata tgcaaataga acggcaattt atttacgaca atccgatctg
    21721 ttttggcgaa gagtcgctat tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc
    21781 cgattccgcc cgcattcatg ttcgttttac gctgaccaat ggcaacaacg aagagcaaga
    21841 actggtttta cagcggcgcg aaggcaagtg ggaaatcgct gattttatcc gcccgaacag
    21901 cggcagccta cttaagcaga ttgaggcaaa aactgccgcc agattaaagc aatgagctga
    21961 attaaataac aattagccgg aacaataaat aaaagggaac actatatgaa aacgattttc
    22021 accgtgggag ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag
    22081 gtcaatcagc tggcgagcaa tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag
    22141 gatatgaaag cactacgccc acaaatctat gctgccaaat ccgaagctaa cagagccaat
    22201 acgcgtcttg atgctcagga ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa
    22261 tgataaaaaa atccccggta gcgtgtcagt tgccggggat attttttaac gtccaaccgc
    22321 cgctttaggc cgtttcttcg caccagcatt caccggacga gattgcgtag acgacgcttt
    22381 ttttgccgta gcaggcgtct gacgctgggt cgccatcggc gtatgtttcg tcaacgccgg
    22441 acgggtattg cggttctggc gacgagcttc acgcatctct tcaatggttg gcgcaggcac
    22501 taagcaatcg cgacggctgc caatcagatg ctttttgccc atcgcttcca gcgcctggcg
    22561 gattaacggc cagtttgccg gatcgtggta acgcaacaac gctttatgca aacgacgctg
    22621 tttgtcgccc ttcggtacga agacgtcttc gctcttataa ccaatcttcg ccagcgggtt
    22681 tttcccggtg taatacatgg tggttgagtt cgccagcggc gacggataga agttctgcac
    22741 ctggtcgagg cggaagcggt gctttttcag ccacagcgcc agattcacca tatcttcatc
    22801 acgcgtaccg gggtgcgcgg agatgaaata ggggatcaga tactgctctt tacctgcctg
    22861 ttttgagtaa gtatcgaaca gctctttaaa gcggtcatag ctgcccatgc ccggcttcat
    22921 catcttcgat aacggccctt cttcggtatg ttccggggca atcttcagat aaccgccgac
    22981 gtgatgggtc gccagttctt tgatatagcg cggatcttct acggctatgt catagcgcac
    23041 accagaggca atcaggatct ttttaatgcc tttcagatca cgcgcacggc gatagaggtt
    23101 gatcgttggt tcgtggttcg tgtccatgtg cggacaaata tccggataaa cgcacgacaa
    23161 acggcgacaa gtttgttcag cgcgtggcga tttgcagcgc aacatataca tgttggcagt
    23221 tggcccacca agatcggaaa tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc
    23281 gatctcatta atgatcgaat cttcggaacg gctctgaata atgcgccctt cgtgctcggt
    23341 gatagaacag aaagagcagc cgccaaagca gccacgcata atgttgaccg aaaaacggat
    23401 catttcgtaa gccggaatac gggcattgcc ataggccgga tgtggcacgc gcttgtatgg
    23461 cagcgcaaaa acgctgtcca tctcttcggt agaaagcgga atagcaggcg ggttgatcca
    23521 cacatagcgg tcgccgtgtt tttgcatcaa tgcgcgggca cagcctgggt tggtttcgtg
    23581 gtgcagaata cgcgaagcat gggcgtacag cactttatcg cccttcactt tctcgaaaga
    23641 aggcagcaac acgtaggttt tttcccacgg tttcgggcgc ggtggctgca cggttacggc
    23701 tttggcttcc tgctttttcg gtgccaccgg tttgttatcc gcgcacggca aatcttcacc
    23761 atacggatgc gggattgggt cgatttttcc aggggtatca agacgggtgg aatccacgcc
    23821 gctccagcca ggcagcgcct ctttcacgat aatcgcggta ttacgcacat cgcggatttc
    23881 actaattggc tcgcccatcg ccagacgatg cgccacttcc accagcggac gctcaccgtt
    23941 accaaacatc agcatgtcgg ctttcgaatc caccagcacg gaacggcgca cggtatcgga
    24001 ccagtaatca taatgcgcgg tacggcgcag actagcctca ataccgccga ggatcaccgg
    24061 tacatctttc cacgcctctt tacaacgctg ggtataaacc agtgtggcgc gatccgggcg
    24121 cttacccgcg acgttatccg gcgtgtaggc atcgtcatga cgtaaacggc gatcggcggt
    24181 ataacggttg atcatcgaat ccatgttgcc agcagtaaca ccgaaaaaca gattcggttt
    24241 acccagacgc ataaagtcgt ctttgctgct ccagtccggc tgggcgatga tcccgacgcg
    24301 aaagccctgc gcttccagca tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac
    24361 atacgcgtcg ccagtaacca aaatgatgtc gcagctatcc cagccaagtt gatccatctc
    24421 ttcacgagac atcggcaaaa acggtgccgg tccaaaacag gcggcccagt actgcggcca
    24481 ggagaacagg tcgcgatccg gttggatcag ggagatagag ctcattttgc ttccagaaat
    24541 gataaaaaaa taatcaaagg ccggggatta taagccggaa cgaaagagaa atcgaaaggt
    24601 attccatact cgccctcctc gggcgagtat gaagattacg gtaccggatt gaccaacagt
    24661 tgcccaatcg agccacggtc cgccatttcc agcgtctgac tgttgaagta gaacgggaag
    24721 tgcgcccagg aaggctgacc gaaataaaca agcagctcca cttgtccgtc aacccaaacg
    24781 gtatctttcc agcctctgtc ttccgggaac ggcatcgcgc cattcacgtt acggatctgg
    24841 aacattacgc cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc
    24901 cacgttcctt gctgcgcggt gacatcaata cggttgacgt cccacagctg tccattaata
    24961 cccgggtcat cacccagact gatatcgcga ctgcgaattg gcgaaccagc catgatttca
    25021 gttggcagca agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc
    25081 agcgttagca ccagggtaga aaccagaata ctggatggct caaagaagcc acgaatacga
    25141 tcaacaatgc tcgccgcttc gccacaggtg atcgacactt catcgccgtt gctcatatcc
    25201 accagaatct cgcggcgctc gcccggtgcc agcgaaagtt gcttcaccga tacaggagca
    25261 ggcaggaatc cctgatcgcc agaaatcaca tgtaacgggc gaccatcgtt catctgtagt
    25321 tgatagcgac gagagttcga cgcgttcagc agtcgcaagc gcacccagcc acgcgagact
    25381 tcaacgtacg ggctttgtac accgttaacc agcagcgtat caccaacaaa gccgccgctt
    25441 cccggttcgt tgtattctgg cgtaccaaag ttatccagcc gtttatcctg gataatgacc
    25501 ggaaaatcat ccacaccata atggttgggg ataggcagcg acttgctgac ttcatcttcc
    25561 accagccaca ttccggcaag gccgttatag acctgctgag ccgtgcggtt gggagtattg
    25621 gcgtgatacc acagagtagc tgcgttctgg cgaatgggca gtacgggtgc ccagtcagcg
    25681 tttggcgaca tcatccgtgc cggaccgccc atcagcgggc ctggtacctg tagcccggcc
    25741 accgtcattg agacattttc tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc
    25801 ttccagacgc ggatagtcgg ccccaggtaa cgaccattga ttccccagac cgacgcgcgt
    25861 gtccctggcg taaatgacca gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg
    25921 cgagattcaa gtagcggcgg aacgggtagc ggttgctgtt gcccggctgc gctggccttc
    25981 aggggaacag cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg acgccgactg
    26041 agtgacatat ttgctccatg taaaactggc taaagtaata ccgtgcagga aagtgttccc
    26101 cgcaaaaata cagattgccg acttaaactt ttccggcggc ttcgcgttct gcgacttctt
    26161 tatcgagctc ggcgattttt tgttccatta tcgaacgaca atgggcagcc agctcacgaa
    26221 cctgatcttt gccatactga ctgacgtcaa ttggcggcag catttcgaca atcaccagac
    26281 cgttgtgcag tcgattaaga ttaatcttat tcgaagttgt agagacgcac acgggaataa
    26341 tcgggacgcc cgccgcaatt gccgcgtgaa atgctccagt cttgaacggt agcaggccgc
    26401 gaccacggct gcgggttcct tccgggaaca tccagatgga aatacggcgt tttttgaagt
    26461 gattcactac ttccgcaatg gtgccgtgag ctttagtgcg attgtttctg tcgatcaata
    26521 agttgccggt taaccagtac aactgcccga agaaggggat ccacagcaag ctctttttac
    26581 ctaccgtcac cgtcggcggt tgcacgatgt tcgatgctgt caccatgtca tagttgttct
    26641 ggtggttagc gatatagata gcattgccgt agctttcagc gtctgtaggt ttacggcact
    26701 caactttcag gccaaacagc ggcgcaagac ggccaaacat atgcccaaag gtggccacat
    26761 gtttcgggtt acgcgggctg aaaaggcagt aaatggagcc gaatacacag actaagatgc
    26821 tgtaaatcac ggtaataata agacgaaaga tatatagcat aacaccctct gaaggtctga
    26881 gagactggca ttgtacgtca cctgaaatca ggttagggca ttgttaaaag ccgctctctg
    26941 gaaaagcgta ttgttaatcg caacgcatga ataaacaacg gtttcaaggg aatttttatc
    27001 gtttccgggg gttgaatacg ctgccggatg acgacttaac gtttcatccg gcgttccttg
    27061 caagcgggag gaaacagcgc cctccccggc atattactct tcgctatcac cgctgctggc
    27121 acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca tcaacgtacc
    27181 gcggcgtcca cgttcgccag tgactttctg taactcttcc gggcgcagtt taattttgcg
    27241 tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacaatt gcgccagacc
    27301 atcttctcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc ctttgccctt
    27361 cgacagctgc ggcagatcac ttaccgggaa catcaacata cggcctgcct gagtgattgc
    27421 cagcagcata tcggaagcat cttcaatcac caccggcggc ataacatggg cattttccgg
    27481 taaggtgatc aaagccttac ctgcacggtt acgcgccacc agatcgttaa aggtgcagac
    27541 gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt ccatcagcat
    27601 atggtcaacg gtcgccccag gcggcaacgt taatttgccg gtgagcggct cgccctgacc
    27661 acgcgccgac ggcagcgtaa tcgggtcaat ggcatagcta cgaccggtgg aatcaacaaa
    27721 cactaccggt tggttgctct tacctttcac cgccgctttg aagctatcac ccgctttata
    27781 attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc ccatctgcga
    27841 cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc
    27901 ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag
    27961 ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct gcaactggtc
    28021 gcgctctttt tccagttcac tctgctcacc gcgaatcttc atctcttcca gtttggcaag
    28081 atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga
    28141 catcagcgcc ggtttcggtt catcttcatt acgaatgatc tcaatcactt cgtcgatatt
    28201 gagaaacgcc accagcaaac cttcgaggat atgcaggcgc ttgaggactt tctccagacg
    28261 atagttcagt cggcggcgca cggtatcgcg gcggaacacc agccattcgg agaggatttc
    28321 cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattaaggt taatacgata
    28381 gctcttttcc agatcggtgg tagcgaagag gtggttcatc acctgatcca tatccacgcg
    28441 gttggaacgc ggcacaatca ccaggcgggt cgggttctcg tggtcagatt catcgcgcag
    28501 atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct ccagtacgcg
    28561 cgcacctgaa acctgatgcg gcaatgcgct gataaccacc gcgccatctt ctttcttcca
    28621 caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgga tctcggcgcg
    28681 cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat ccagcagctg
    28741 atcgagcgtg gttttcggct ggtcgattaa tgcgattgcc gcctgagcca cttcacgcag
    28801 gttatgcggt ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa
    28861 aatgtttggc agacgggcag gtagcatttt cggctcctgc aaagtgccgt cgaagtttgg
    28921 cacccagtca gccgtcccct gccccagctc gctcaatagc agctcggaat atttcgacaa
    28981 ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt
    29041 cccctgacca tcaaccagcg gataacggta agagaacggt tgcgccatca ggaccatcgc
    29101 ttcataacag gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac cgacggtacg
    29161 ggccgatttt ttaaatttgg cgctggcatt caggcccagt tcagacatcg catacacaat
    29221 gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt ccatgatcac
    29281 gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc
    29341 catatcgctc attaattctg attcctcaac ttattcgcca gccttcaaag gggcaatatt
    29401 gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg
    29461 ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa ctctttgctg
    29521 tagacataat cctggggatt atgcagcgga aaaccgccaa cgtagggttt aagcagtttg
    29581 attaacggct gatagtagat aggaatcagc ggtgcctgtt ggttgatgat cacttctgcc
    29641 tgctgataca acgcattacg ctttgtcgca tcagtgatct gcgtggcctg gtttagtaag
    29701 gcgtcatact gcgcattttt ccagtgaccg acgttttctt cactatcgct tttgagcgtg
    29761 ttcaggaagc tggaagcatc attgtacgtc gcatcccacg actgccgaga cagcatgaaa
    29821 tcaccggctc gtcgggcatc aagataggtt ttccactcca ttgtgcgcag cgtcacctgt
    29881 gcacccagcc attttttcca ttcggaagac aacgctatcg cggtcttttc atgcagatcg
    29941 tacttgttgt agaacagctc aaagcgtagc ggatgagagg cgtcgtatcc cgcctgtttc
    30001 agcaaggctt ttgccatcgc gacgcgctca ctcattggct tttgcagttc atcgaacgtc
    30061 gtcgcgctaa agccttttac ctctggcggc gtcagcgtgg ttgcgggcgt tctcaacccc
    30121 agtacctttt gcgcaataag ctgtcgatca accgtaagat atagcgcccg acgcactcgc
    30181 acatcgttaa atggcggttt ctcaaggttg aagttgtaat attcgctgtt cagacgcgga
    30241 ataattcgta gctcgccagg cagtgatttt tcaatggcgg gaatttgctg cgccggaacc
    30301 caggtgagat cgacctctcc cgcgcgatag cggttatagc cggtgaccga attatctagc
    30361 gccagatact caacctgttg caatactgta tgttgcgcat cgcggtactt tggatttttg
    30421 cgtgcagtaa tcttttcgtt aactacccac tgatcaagca caaaggcacc gttgtaaacc
    30481 atgttctctg gcttactcca gctatcgcca tgtttagcga tgacatgatg aggaaccggg
    30541 aacagcgtcg gccaggcgag catcgtcgtg aaccaaggaa ccggctgctc aagcgtaact
    30601 tcaagagtac gatcatccgt cgctttgaca cccagcgatg taacatctgc tttacccgca
    30661 acaatagctg cggcattgtt aatgtgcgcc tgtgccagat agccagcaaa agggcttgcc
    30721 gttttcgggt caaccgcgcg ctgccagccg aggacaaaat cctctgccgt cagaggctga
    30781 ccgtcggacc actgcaaacc gctacgcaga tggaaaatat agcgcttgcc gccgtccagt
    30841 atctcccagc gttcagcctg agcgggctgc acctggcctt caccgtccat ccataccaga
    30901 ccttcaaaca gatccagcac aatctgcgcg gcagtattct cctccacctt ttgcgggtcg
    30961 agcgtacctg ggtcgctatg attgttgtaa cgaaagactt gttgcggggc gagcggtgtg
    31021 ttggcgggaa cgtcagcagc gtagagagga gccgcactta ccaggctgac cagccataat
    31081 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    31141 ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagaaa
    31201 aaagcgagtt gcccccgctt ttccattaat taacggattt cattcaatag ttctggatgc
    31261 ttatccagaa cacgtaaaag tttgattgtg gaaggatgag gttgggcatt gcctttttcg
    31321 taacgcgaaa acgcatttac acctccccca aaaatttcgc ttgcctcttt ttgggtaaga
    31381 gagagctttt ttcgaacctt cactataaat tcaggtgcca ctgtttcggc attcaccgaa
    31441 gcccgaaatg cctttacttg cgccatgaaa gcatctgact cttctttatt catgatgctc
    31501 tcttcgcaat ggacacaata taaaccgtgg atacctttca atactgtttt tcgtccacgg
    31561 aaggtgtatg gaatatcttt aatgccagaa accatttctc cctggtggca aaccggacat
    31621 ttcatattac ttctccttaa acgagacgat cagtacgtca tgaattaccg taattttaag
    31681 ataaacctgg cctgtaacaa gcctgggtct gtaaacatcc tgccagatag tatgatcaga
    31741 gtaggtggtc atgcttttat aaaagtcgct ctctgataat ccaatgataa cattacacat
    31801 accatcaaaa tccaaaccta actcatctgc atttaacagg gcactacgtg ttgtacgaac
    31861 ttgcccggca ttgacaagtt ttttaacctg actcaaacgt gtatgtggtg tgcgtttttc
    31921 cataaccccc gcctccctgt tactttagtt ataacctaaa aggttaatta cagcaatgaa
    31981 aaagcaccta aaaggttagt tagatgtacg gagatagtga ccacacaaaa cgtattcttt
    32041 aaggaaagtg attgaccata taagaaagtg gcgcattagt agcgccagtt tgaagcagga
    32101 atttataagg gaagctggag tcaggcatca cgccaacggc acataaacat ccgtttccag
    32161 tcgctgctct gtcacccctt cggcaagatt ggtgtagtga aacagaatcg gtgctttacg
    32221 cattttctcg ccgcttgcag gcagccagtg gcgaatgatg ccccataccg tgtgtgaaat
    32281 atcgtccagc tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa gttcaccatt
    32341 gctcacacca taacgattat cgggaatcgg ttcgctaacg ctgccgcaga tatcaaagcg
    32401 aaacgcttcc ggtgcggtgg tggcagggtc gtcccaggcg acgccaaaag tctggctttg
    32461 gttaacagga gaaagccccg tctctttacg ccacatgata aatttcgcca cgctgtagtt
    32521 gagcaattca gggctacagc ggtgcggcaa catcgccaca ggtattgatg ggaaatcgat
    32581 aatgtttaca tccagtgtca ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa
    32641 cgcgtcccgg gagcggtaac aattacggat ttactttgcg gatctgtttg acgtcaattt
    32701 caacagaatt ccagtcttta tcgacttcac cctgaatctc aaccgtatct ttcggcgtca
    32761 ccgtcacgcc gttccagcgt ttgtggtcga tatcaacatt gatagtaccg ctggcatctt
    32821 tgaacacgta gagatcgtca gagatgcgtt caacgatatt gccgcgcagg gttacccagg
    32881 tgtcgtcacg cagggatttt gcgctttcta cagtcgttac gctgccgttc ggcccctgga
    32941 atcctccggc ctgactttgc gttgccgatg ggccagaaaa accgccctgc tctgctgcca
    33001 tcaccggtgc gctgcacagg gccattactg cgattactgc tgcgaatttt ttcatgttta
    33061 ttactccctt taatgtctgt ttccgagcat ttaacaagat agtccttaac aacttcttaa
    33121 gggaaaaaaa taaaatttag tgctgtacag agcgcgttac aacacggttt actggcagca
    33181 aatacggtta tcgcagggat gaaaaaatgc gaattttact gatagaagat gacatgctga
    33241 ttggcgacgg catcaaaacg ggccttagta aaatgggttt tagcgtcgac tggtttacac
    33301 aaggtcgtca gggaaaagag gcgctttata gcgcacctta tgatgcggtg atcctggatt
    33361 taaccttacc aggcatggat ggtcgcgata ttttgcgcga atggcgagaa aaaggtcagc
    33421 gtgagccggt actgatcctg accgcgcgcg atgcgctggc ggaacgtgta gaagggctgc
    33481 gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc
    33541 tggaagctct gatgcgccga accaacggcc aggccagcaa cgagctgcgc cacggtaacg
    33601 tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg cgaaccctta acactgaaac
    33661 caaaagaatt tgccctgctg gaattactga tgcgtaacgc tggtcgggta ctgccgcgca
    33721 aactgattga agagaaactg tatacctggg acgaagaggt caccagtaat gccgttgaag
    33781 tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt tattcgtacc gtgcatggta
    33841 ttggttacac attaggtgag aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct
    33901 gacgctaatc tttttaattc tggcctcggt gacctggctg ctttccagct ttgtcgcctg
    33961 gaaacaaaca acggataacg tcgatgaatt gttcgacacc caactgatgc tgtttgccaa
    34021 gcggttaagt acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc
    34081 aaatagatta aaacacggtc atgttgatga cgatgcgctg acctttgcca tctttaccca
    34141 cgacggcaga atggtcctta atgatggcga taacggagaa gatattccct atagctatca
    34201 acgggaaggt tttgctgacg ggcaactggt cggtgaagac gatccttggc gttttgtctg
    34261 gatgacctca cctgatggca aatatcgcat cgttgttggc caggaatggg aataccgtga
    34321 agacatggcg ctggcgattg ttgccgggca attgatcccg tggctggtcg cactgccgat
    34381 tatgttaatc atcatgatgg tactactggg tcgtgaactc gcgccgctga acaaactggc
    34441 gctggcacta cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga ctggcgtacc
    34501 cagcgaagtg cggccactgg ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat
    34561 gatggttcgt gaacgacgct ttacctccga cgcagctcac gaacttcgta gcccgttaac
    34621 ggcgctgaaa gtgcaaaccg aagttgcgca gctctctgac gatgatccgc aggcgcggaa
    34681 aaaagcactg ctccaattac attccgggat cgatcgcgct actcgtctgg ttgatcaact
    34741 gctcacgcta tcgcggctgg actcactgga taaccttcag gacgtcgcgg agatcccgct
    34801 tgaagatctc ctgcaatcgt cggtgatgga tatttaccac acggcgcagc aggcgaaaat
    34861 tgacgtgcga ctgacactca atgcccacag catcaaacgc accgggcaac cgctattgct
    34921 aagtttgttg gtgcgaaatt tgctggataa cgccgtgcgc tacagtccac agggcagcgt
    34981 ggtagacgtc acgctgaatg ctgataattt catcgtgagg gataacggcc ccggtgtgac
    35041 accagaggca ctggcgcgaa ttggcgaacg cttctatcgc ccacccggac aaaccgctac
    35101 cggcagcggg cttgggctat cgattgtcca gcgaatcgcc aaattgcatg gcatgaatgt
    35161 tgaatttggg aatgcggaac aaggtggatt tgaggcgaag gtaagctggt aacttctctg
    35221 tctatgagag ccgttaaaac gactctcata gattttacta atagcaaaat ataaaccgtc
    35281 cccaaaaaag ccaccaacca caaaccagac aaataccgag cgagaataca tatacccaaa
    35341 actcgaacat ttcccgcata aagagttttc ttaagataag aataataagt ggcgtaagaa
    35401 gaaaaaatgc tgcaatcatt gcttgcaata tacgatcttc gcctgaaacg gaaggtatac
    35461 tgcaaatatt tcctcccgac tcaagcatcc agtcatactt attgaaagca aaaaaa
//
LOCUS       DAYPHN010000032        35070 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000032 DAYPHN010000000
VERSION     DAYPHN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.032"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            168..1385
                     /gene="alaA"
                     /locus_tag="ACG0BW_001532"
     CDS             168..1385
                     /gene="alaA"
                     /locus_tag="ACG0BW_001532"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFK7258512.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1469..2068
                     /gene="yfbR"
                     /locus_tag="ACG0BW_001533"
     CDS             1469..2068
                     /gene="yfbR"
                     /locus_tag="ACG0BW_001533"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFK7258513.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2127..3959)
                     /locus_tag="ACG0BW_001534"
     CDS             complement(2127..3959)
                     /locus_tag="ACG0BW_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFK7258514.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACG0BW_001535"
     CDS             complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACG0BW_001535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFK7258515.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKVAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4707..5201)
                     /locus_tag="ACG0BW_001536"
     CDS             complement(4707..5201)
                     /locus_tag="ACG0BW_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFK7258516.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACG0BW_001537"
     CDS             complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACG0BW_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFK7258517.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6077..7279
                     /gene="ackA"
                     /locus_tag="ACG0BW_001538"
     CDS             6077..7279
                     /gene="ackA"
                     /locus_tag="ACG0BW_001538"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFK7258518.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7354..9498
                     /gene="pta"
                     /locus_tag="ACG0BW_001539"
     CDS             7354..9498
                     /gene="pta"
                     /locus_tag="ACG0BW_001539"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFK7258519.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9688..11208
                     /gene="yfcC"
                     /locus_tag="ACG0BW_001540"
     CDS             9688..11208
                     /gene="yfcC"
                     /locus_tag="ACG0BW_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFK7258520.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACG0BW_001541"
     CDS             complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACG0BW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFK7258521.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACG0BW_001542"
     CDS             complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACG0BW_001542"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFK7258522.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACG0BW_001543"
     CDS             complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACG0BW_001543"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFK7258523.1"
                     /translation="MSKPAITFWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13228..13875
                     /gene="yfcG"
                     /locus_tag="ACG0BW_001544"
     CDS             13228..13875
                     /gene="yfcG"
                     /locus_tag="ACG0BW_001544"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFK7258524.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLEYSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13932..14294
                     /gene="folX"
                     /locus_tag="ACG0BW_001545"
     CDS             13932..14294
                     /gene="folX"
                     /locus_tag="ACG0BW_001545"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFK7258525.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14315..15208
                     /locus_tag="ACG0BW_001546"
     CDS             14315..15208
                     /locus_tag="ACG0BW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFK7258526.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSSRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATVIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15256..16146)
                     /gene="rpnB"
                     /locus_tag="ACG0BW_001547"
     CDS             complement(15256..16146)
                     /gene="rpnB"
                     /locus_tag="ACG0BW_001547"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFK7258527.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16342..17115)
                     /gene="hisP"
                     /locus_tag="ACG0BW_001548"
     CDS             complement(16342..17115)
                     /gene="hisP"
                     /locus_tag="ACG0BW_001548"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFK7258528.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17123..17839)
                     /locus_tag="ACG0BW_001549"
     CDS             complement(17123..17839)
                     /locus_tag="ACG0BW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7258529.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVMALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17836..18522)
                     /gene="hisQ"
                     /locus_tag="ACG0BW_001550"
     CDS             complement(17836..18522)
                     /gene="hisQ"
                     /locus_tag="ACG0BW_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFK7258530.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18612..19394)
                     /gene="hisJ"
                     /locus_tag="ACG0BW_001551"
     CDS             complement(18612..19394)
                     /gene="hisJ"
                     /locus_tag="ACG0BW_001551"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFK7258531.1"
                     /translation="MKKLVLSLSLVLAFSSATVAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19615..20397)
                     /gene="argT"
                     /locus_tag="ACG0BW_001552"
     CDS             complement(19615..20397)
                     /gene="argT"
                     /locus_tag="ACG0BW_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFK7258532.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTVDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20663..21232)
                     /gene="ubiX"
                     /locus_tag="ACG0BW_001553"
     CDS             complement(20663..21232)
                     /gene="ubiX"
                     /locus_tag="ACG0BW_001553"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFK7258533.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADVETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21327..22844)
                     /gene="purF"
                     /locus_tag="ACG0BW_001554"
     CDS             complement(21327..22844)
                     /gene="purF"
                     /locus_tag="ACG0BW_001554"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFK7258534.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22881..23369)
                     /gene="cvpA"
                     /locus_tag="ACG0BW_001555"
     CDS             complement(22881..23369)
                     /gene="cvpA"
                     /locus_tag="ACG0BW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFK7258535.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23628..24290)
                     /gene="dedD"
                     /locus_tag="ACG0BW_001556"
     CDS             complement(23628..24290)
                     /gene="dedD"
                     /locus_tag="ACG0BW_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFK7258536.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKRKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24280..25548)
                     /gene="folC"
                     /locus_tag="ACG0BW_001557"
     CDS             complement(24280..25548)
                     /gene="folC"
                     /locus_tag="ACG0BW_001557"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFK7258537.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQEQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25618..26532)
                     /gene="accD"
                     /locus_tag="ACG0BW_001558"
     CDS             complement(25618..26532)
                     /gene="accD"
                     /locus_tag="ACG0BW_001558"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFK7258538.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26688..27347)
                     /locus_tag="ACG0BW_001559"
     CDS             complement(26688..27347)
                     /locus_tag="ACG0BW_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFK7258539.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27430..28242)
                     /gene="truA"
                     /locus_tag="ACG0BW_001560"
     CDS             complement(27430..28242)
                     /gene="truA"
                     /locus_tag="ACG0BW_001560"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFK7258540.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28242..29255)
                     /locus_tag="ACG0BW_001561"
     CDS             complement(28242..29255)
                     /locus_tag="ACG0BW_001561"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFK7258541.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATGAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29321..30457)
                     /gene="pdxB"
                     /locus_tag="ACG0BW_001562"
     CDS             complement(29321..30457)
                     /gene="pdxB"
                     /locus_tag="ACG0BW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFK7258542.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLGGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30556..31551
                     /gene="flk"
                     /locus_tag="ACG0BW_001563"
     CDS             30556..31551
                     /gene="flk"
                     /locus_tag="ACG0BW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFK7258543.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILTLVWLVR"
     gene            complement(31548..32726)
                     /locus_tag="ACG0BW_001564"
     CDS             complement(31548..32726)
                     /locus_tag="ACG0BW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFK7258544.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33011..34231)
                     /gene="fabB"
                     /locus_tag="ACG0BW_001565"
     CDS             complement(33011..34231)
                     /gene="fabB"
                     /locus_tag="ACG0BW_001565"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFK7258545.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34390..>35070
                     /gene="mnmD"
                     /locus_tag="ACG0BW_001566"
     CDS             34390..>35070
                     /gene="mnmD"
                     /locus_tag="ACG0BW_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HFK7258546.1"
                     /translation="MKHNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFVQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEKLQAQWPLPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTM"
BASE COUNT         8655 a         9592 c         8678 g         8145 t
ORIGIN      
        1 cacctcatta ttttgccatt attaagttat taacagcaca atcgagtctt cccctctggc
       61 aaaatcttat tctgcagacc ttcaaaacgc cgtcctgggg gagtacattg ttctaagctg
      121 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
      181 aaaaatccag caaattagag aatgtctgtt acgacatccg tggtccggtg ctgaaagaag
      241 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aaccctgccc
      301 cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt gattcgcaac ctgcctaccg
      361 ctcaagggta ttgcgattcc aaagggcttt actccgcgcg taaagccatc atgcagcact
      421 accaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
      481 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggcgacgaa atgttggttc
      541 ctgcacctga ttacccacta tggaccgcgg cggtttcgct ctccagcggt aaagcggtgc
      601 attatctttg cgatgaatcc tctgactggt tcccggacct tgatgatatt cgcgctaaaa
      661 ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa taacccaact ggcgcggtat
      721 attccaaaga gcttttaatg gagatagtgg agattgcacg tcagcataat ctcattatct
      781 tcgccgatga aatttatgac aaaattctct acgacgacgc tgagcatcac tcaattgcgc
      841 cgctggcacc tgatctgctg actattacct ttaacggact gtcgaaaacg taccgcgttg
      901 ctggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggttaca
      961 tcgaaggtct ggaaatgctg gcctcaatgc gcctgtgtgc taacgttcct gcgcaacacg
     1021 ccattcagac cgcgctgggc ggttatcaga gcatcagtga atttattacc cctggcggtc
     1081 gtctttatga gcagcgtaac cgcgcatggg aattgatcaa tgatattccg ggcgtttcct
     1141 gcgtgaaacc gcgcggtgcg ctgtacatgt tcccgaaaat cgacgccaaa cgctttaata
     1201 ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
     1261 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
     1321 tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
     1381 tgtaatctta atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc
     1441 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
     1501 ccgcctgaaa ctcattaacc gctggccgct gatgcgcaac gtgcggacgg aaaatgtgtc
     1561 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
     1621 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
     1681 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca
     1741 ggagtacaag gcgattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga
     1801 gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
     1861 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
     1921 gggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgccg
     1981 cagccaggag atggactact tcatggaagt ctttgttccc agcttccatc tttcgctcga
     2041 tgagattagc caggattcac cgctgtaagc agccggagtc tgcgtcgcat caggcaataa
     2101 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
     2161 caaaccgcca tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg
     2221 taatttcccg gacctaaaac cagtgtgtta acaggtgaag aaaccggcgt cataaaggcg
     2281 gcggatgctg ccatcgccac gaccatcgca aatggataag gcgacacacc catcgttttg
     2341 gcagcagcca gcgcaatcgg agccatcaac accgccgttg cggtattaga gataaatagc
     2401 ccaataaccg ccgacaagac aaacaaacaa cccagcatca tatgtggccc gtaaccgccg
     2461 ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
     2521 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
     2581 ctttcagcat cgatacaacg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
     2641 ggattaggaa tttcatcggt cagcattaac gccaccatta gcaccagaca gaaaatcgca
     2701 tggggtgcct ggctgtgcgc gggcgatgct tcactcactt cttctggtaa gttcagcgcc
     2761 acaaagtcac gcccctgttt tgccagcata ccgatcagtt tccagttacc cacaaccagg
     2821 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccatcgc ctcgccattg
     2881 cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
     2941 ttaccaataa gttcagactc aggaattaat gaaatctctg ccatgcccac atcaagtgcc
     3001 tggtcagaaa aatactcgcc gcgtagtacc atcggctcca gcaactgctc actacaaaat
     3061 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
     3121 tcagaaaccc cattaacgtt cacgataaca cgacgaaaac gccgccagcg ttcaacaccg
     3181 atgacgttag cgccataacg ctcacgtaat ttgagatcgt ccagccgttg accaatcatt
     3241 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
     3301 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
     3361 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
     3421 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
     3481 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc ttatcagccc ggcaaaactt
     3541 aacggcatca tcaggcgcga cggcgacgtt tgcatacgca tggcaacgct taacaccacg
     3601 ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc
     3661 agcatcaaca aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtc
     3721 cccattacag tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
     3781 atcaggacaa cgttaggatc ggaaaagccg gaaaatactt cggggaccgt cagcgttccg
     3841 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
     3901 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
     3961 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
     4021 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
     4081 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
     4141 ctgccggagc gttaaccgca atgacatgac aacccgccgc caggccagaa agcacgccag
     4201 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcgccta
     4261 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
     4321 ctggtgcggg aagcccagct actttatggc gcgctcgcgc taccggcatg gagccagaag
     4381 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg
     4441 gaagcgcggt aataccctcg gtttccgtgg cctcgatttg ctccagacgc gtaaactcgg
     4501 cggcaatatc agcctcggat ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt
     4561 taccgtgaat aaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
     4621 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
     4681 aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
     4741 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
     4801 cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct
     4861 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
     4921 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
     4981 acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc
     5041 agctcgccaa actcgcgatc cagttcacgc atctgtaatc cataaccacg ctcaatgatt
     5101 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
     5161 tggttagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
     5221 ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
     5281 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc
     5341 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
     5401 caatttaccg cgaacttcat aaaaccagtt gaggatagca gggggtaatg gcgttacaga
     5461 acgtttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gcgcagtggc
     5521 aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat
     5581 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
     5641 cggggcaagg cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa
     5701 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
     5761 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
     5821 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
     5881 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
     5941 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
     6001 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
     6061 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
     6121 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
     6181 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
     6241 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
     6301 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
     6361 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
     6421 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
     6481 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
     6541 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
     6601 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
     6661 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
     6721 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
     6781 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
     6841 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
     6901 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
     6961 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
     7021 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcaccg gtggtatcgg
     7081 tgaaaatgcc gcgatggttc gtgagctgtc tctgggcaaa ctgggcgtgc tgggctttga
     7141 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
     7201 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
     7261 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
     7321 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
     7381 accggaacca gcgtcggtct gactagcgtc agccttggcg tgatccgtgc aatggaacgc
     7441 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
     7501 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
     7561 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
     7621 gagatcgtcg cgaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
     7681 ctggtcccga cacgtaaaca ccagtttgcc cagtctctga actacgaaat cgctaaaacg
     7741 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
     7801 gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc
     7861 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
     7921 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
     7981 caagaatcca gcccgctgcc ggttctcggc gcggtgccgt ggagctttga cctgatcgcg
     8041 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
     8101 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
     8161 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggttgcc
     8221 gcttgcctgg ctgccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
     8281 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
     8341 tttatggtga acaccaacac ctggcagact tctctgagcc tgcagagctt caacctggaa
     8401 gttccggttg acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc
     8461 aacgctgact ggatcgattc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
     8521 ccagcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgttctg
     8581 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
     8641 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
     8701 gtagaactgg gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
     8761 ggtcgtctgg tcgaactgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
     8821 ctggaagaca acgtggttct cggtacgctg atgctggaac aggatgaagt tgatggtctg
     8881 gtttccggtg ctgttcacac caccgcaaac accatccgtc cgccgctgca gctgatcaaa
     8941 acggctccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
     9001 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg cagaacagct ggcagaaatc
     9061 gcgattcagt ccgcagattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc
     9121 tcctactcca ccgggacttc tggtgctggt agcgacgtag aaaaagttcg cgaagcaact
     9181 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
     9241 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
     9301 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
     9361 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac
     9421 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
     9481 tctgcacagc agcagtaatc tcgtcatcat ccgcagcttt gcgctgcgga tatcggaacc
     9541 ggaaatagtg actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta
     9601 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt
     9661 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaaccaga
     9721 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgccatttt aaccagtctt
     9781 gccacctggg tagttccggt gggcatgttt gacagtcagg aagtgcagta tcaggttgat
     9841 ggtcaaacaa aaacacgaaa agtcgtagat ccacactcat ttcgcattct gactaacgaa
     9901 gcaggcgaac ctgagtatca ccgcgtacag ctgtttacga cgggcgatga acgcccgggc
     9961 ctgatgaact tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc
    10021 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg aacaggaacc
    10081 attgataacg gtattctggc gcttattcgc catacccgcg gaaatgaaat tctctttatt
    10141 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc
    10201 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    10261 accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    10321 ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccggattg
    10381 cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc
    10441 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    10501 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    10561 gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    10621 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    10681 ttccgcctta acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga
    10741 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt
    10801 aatggtgaag cgggtgatgc cagcgtgtta aataccatcc tcaacagcat tgccaatgcc
    10861 attagcggtc tggacaatgc ggtcgcggcc tggtttatgt tgctcttcca ggcagtattt
    10921 aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg
    10981 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggcgttcca gtttggtgat
    11041 ggcttcagcc acatcattta cccgacctca gcttcgttaa tggcgacgct cggtgtgtgc
    11101 cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt
    11161 atgtccagcg tcgtagtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    11221 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    11281 ttgcgcttca tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    11341 gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg
    11401 aagggaccgt gagagacgca actgaacaac gcgccccaga cacggcaatt tttatcttcg
    11461 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    11521 tcgcgacgcg cggattccag cagttgctca tcggcctgga ccacaccgcc tgcggtcgca
    11581 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    11641 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt
    11701 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    11761 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    11821 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    11881 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg
    11941 cctttaggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc
    12001 actggcagat gggtatgacc gtacaccagc acatcgttct ggtttaaagc aggtaggttt
    12061 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    12121 tgccacggcg cggttatcgg gaaatgcaaa agcatttgat ccacttcgct gtcgcagttg
    12181 ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    12241 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    12301 agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac
    12361 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    12421 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa
    12481 acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttccggcac
    12541 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    12601 ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca tcgcgaacag
    12661 tttctctgca ctggcttttc cctccgccgt taatggcgct tttttcgcac ccgcaaagac
    12721 aacatccgtt ggacgttctt cgcggatggg catcagatcg ctacgcagcc aggcctgaat
    12781 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atgcgttccc aggtcggtgg
    12841 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    12901 aatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg
    12961 ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc
    13021 cacccaggcg gataacacat aaggggagaa aaaatgggca tctgaccaaa acgtgatagc
    13081 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    13141 ttgtcactgt cacgtgacga atactcacgc aagtcgccgc gctttctata cttgtggtgt
    13201 tcagatgatt agacaaccgg agctgcaatg atcgatctct atttcgcccc gacaccaaat
    13261 ggtcacaaaa ttacgctgtt tctcgaagaa gcagggctgg attatcgctt gattaaggta
    13321 gacctgggga aaggcggtca gtttcgcccg gaatttttgc acatttcgcc taacaacaaa
    13381 attccggcaa ttgttgatca ttctccagcc gatggcggcg aaccgctaag cctctttgag
    13441 tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg
    13501 cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    13561 cttgggcaga atcatcattt taatcacgct gccccccaaa ccattcctta cgctattgaa
    13621 cgttatcagg ttgaaactca gcgtctgtac catgtactga acaagcggct ggaatactcg
    13681 ccctggctgg gaggtgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    13741 gcctggactc gccagcgaat tgaccttgca atgtatccgg cagtcaagaa ctggcatgag
    13801 cggatccgtt cgcgccctgc caccggtcag gcactgctaa aagcacaact cggtgatgag
    13861 cgttcggata gttaacagaa acaggttctc gtgtattatt tcattctaag taaaacaacg
    13921 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttacgta
    13981 cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    14041 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    14101 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    14161 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    14221 aagtggagat cgataaactg cacgcgctgc gctacgctga ttcggtatcc atgaccttaa
    14281 gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    14341 gggattaatt ggtcgccatt tgattccacg tttgctggag ctgggccatc aaatcacggt
    14401 aatgacgcgt aacccgcaga aagccagttc tgttctcggc cctcgggtga cactatggca
    14461 agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga
    14521 accgattgct gataaacgct ggactcacga gcaaaaagag cgtctctgcc aaagccgctg
    14581 gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccac cgtcggtact
    14641 catttccggc tcggcaacag gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    14701 ggaaccgccg cataacgaat ttacccataa actctgcgcc cgctgggaag aaattgcctg
    14761 ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcgt accggtgtag tgctggcacc
    14821 ggatggcggt attctcggta aaatgctgcc gccgtttcgt cttggcctgg gcgggccgat
    14881 tggctctagt cggcagtatc tggcctggat tcatattgat gatatggtca acggtattct
    14941 ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccggtacg
    15001 caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcca ttttgcgcgt
    15061 ccccgcgacc gtcattcgac tgttaatggg cgaatcttca gtactggtat taggtggaca
    15121 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    15181 agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact
    15241 cccggtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac
    15301 catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc
    15361 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    15421 tttctccttt tcttgtggtg cgcgttccgc tatctcacca ataaatgcac gaaaacgctg
    15481 ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    15541 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    15601 atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg
    15661 caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg
    15721 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    15781 aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat
    15841 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    15901 ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    15961 ggaataatat tgccgcaggt cgtcatcaat aaaactgttt ggttccagtt taagcgtcgt
    16021 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    16081 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    16141 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgatttaca
    16201 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac
    16261 tgcaactttt acatttcagc ggaagcggac tcgcaaattc cccatgaaat actccggcag
    16321 cgttagccac cggagaggaa attatttcag cgaaccttta aggaactgtt gcagacgagg
    16381 gctttgcggg ttaccaaata actgttctgg cgcgccctct tcttctattt tcccctgatg
    16441 gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag tcactaccac
    16501 catggttttc ccctcttctg ccaattgctg cataatacgt aacacttcgc ctaccagttc
    16561 aggatcgagc gcagaggtag gttcatcaaa tagtaaaact tccggttcca ttgccagcgc
    16621 ccttgcgatg gaaacacgct gttgctgacc gccggaaaga tgcaccggat atttcccctg
    16681 cgcacgttcg tctatcccaa cttttgccag atacttcacc gcccgctcgc gcgcttcctg
    16741 cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccagca ccgtcatatg
    16801 gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagctg
    16861 atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga tcgtctggcc
    16921 gttaaccacg atcgatcctt cgctcggttt ttcgaggaag ttaatgcagc gcagaaaggt
    16981 acttttcccc gatcccgacg atccgatgat gcttatgaca tctccggcat tcgcttgcag
    17041 tgagacccct ttcagcactt cgtgttcgcc gtagcgtttg tgcaaatcga taacgtttaa
    17101 tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc
    17161 gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa tagagcaccg
    17221 cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg cgggctattt
    17281 tcagcagatc cggcacagta gcggtgaaag ccagcgcagt agagtgcagc atcaggatca
    17341 cttcgttgct gtaggccggt aacgcaatac gcagcgccga tggcaaaata atgcagcgat
    17401 acattttaaa ggtcgagaag ccataggctc tggcggcttc gatttcccca tgcggcactg
    17461 aacggatcgc tccggcaaaa atctcggtag tgtaagcgca ggtgttgagc gtcagcgcca
    17521 gcacggtaca gttcaggcca ctgcgaaaaa aggcgttaag gaactcggtt cccttaacaa
    17581 tttccagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc ggcgtaccgc
    17641 gaaaaatata ggtaaataac cagattggaa actggatgta tttattactt gagacgcgac
    17701 caatcgccag aaacagcgcc atgactccgc ctatcactac cgacaaaatc agcagccaca
    17761 aggtgatcgc cacgccagta aagcgataac cgtcagtcca cagcagcggt ttccagtatt
    17821 catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc ggcgctcaag
    17881 gaacagcagc acaccattgg aaacggtggt gaaaaccagg taaatcacgc cacaaacgat
    17941 ggcgaaatag aacggttccc aggtactttt gcctgccagc tgcgtggctt tgaccacatc
    18001 ttccaggcca agtaacgaaa cgagtgcggt agatttgagg atcacctgcc agttgttgcc
    18061 aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa acacttgccc
    18121 acgagtgaaa ccgaacgccg tcgccgcctc tatatgtcct ttcggcactg ccataaaagc
    18181 gccacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtaa taataccagc
    18241 gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcaccg tgtttagtgc
    18301 aatctgcaaa ccgtagaaaa ttagcagcat caacaccaag tccggcacgc ctcgaatcag
    18361 cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct taccgccagc
    18421 gccaattaaa ccgatgatta cagcgagcac tacagagctg atcgccagct ccagcgtgac
    18481 gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg
    18541 tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg gctttctttc
    18601 accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc taatttctcg
    18661 taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta
    18721 tcttctttac gcaggcccat gccggtccct acgccaaaca gtttttcatc tttaacagac
    18781 ggaccaccga atttgtaatc tttaccgacg ggttgtttga ggaaaccttc gctggcagcg
    18841 acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata aatgttgtcc
    18901 tgcccctgat acgagacgat ttcaatgcct tttggtgccc agtgttcgtt accgaacgtc
    18961 tcctgggtgg tgccctgcaa tacgcctacc cgcttgcctt tcagcgactc gactgtcggc
    19021 tgaatgtcag aatttttcgc caccaccaga cgagaatcgg cagcgtacag tttgtcggtg
    19081 aaggctattt cttgctgacg tttttccgta atggaaagcg atgacatgat ggcgtcaatc
    19141 ttcttcgctt ttaaggacgg gattaacgca tccagtgggt tttcgacaaa cgtacattgc
    19201 gtattgatgc gtttgcacaa ctccttcgcc agatcgatat cgaagccaac cagttcgcct
    19261 tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg gatgttttgc
    19321 ggaatcgcag caaacgccac agttgcgctg gagaaggcca gaaccagaga gagcgatagc
    19381 accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat ttacaggttt
    19441 gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc ccgttagacg
    19501 tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat agtgcaacaa
    19561 acctggcgtg caccatgatg atcattccat caggtacagc ttccctgcga cgtatcagtc
    19621 accgtagaca ttaaagtcaa aatacttttt cgccatcttg tcgtaggtgc cgtcctgacg
    19681 cagctcacca agcgccttat tgaaggcagc cgtcagttca gcatcatctt tacgtagccc
    19741 tacaccggtg ccgtcaccga agtatttttt gtctttcact gatgagccag caaaggcgaa
    19801 atctttacca gcaggttgct tgaggaatcc ttcactggca gcaacttcat cttgtaacgc
    19861 agcatccaga cgtcctgcag ccagatcgga ataaaccaaa tcctggttgg cataggccac
    19921 cacatccacg cctttgctac gccaggtctc gttagcgtat gcttcctggg ttgatccctg
    19981 cagcacacca acatgtttac ctttcagtga atccaccgtt ggctgaatcg gtgaaccttt
    20041 ggcggcaatc aaacgagaat ctgcggcgta caacttgtcg gagaaagcaa tctcctgctg
    20101 acgtttatca gtaatggaga gcgacgaaat aatggcgtcg atttttttcg ctttcagtga
    20161 ggggatcagc gcgtcaaagt cactggcaac ccaggtacat ttcacctgca tccgtttgca
    20221 catctcgtta ccgagatcga tatcaaagcc aacaaagtca cctttggcat ctttcgatga
    20281 gaacggcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg ccgcatagct
    20341 ggaagccgct gcggagagac cgactaacaa agacagagcg agaatcgact tcttcataca
    20401 taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc
    20461 tttcatgcag gtcttatgcc atctttacga caacagtaac attcaacgtt aaatatgtta
    20521 acaagacgtt gcattattgt cctaaagttg aagatagcag gtatagcggt tgaatcgcag
    20581 cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg caaaattgcc
    20641 ctgaaacagg gcaacaatga ggttatgcgc cctgccagcg ggcaaagaga tcttcaggaa
    20701 gcgttatggc gaattgatcg agtacacggt taaccgtctg atttatcaca tcatcaaggg
    20761 attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg atttctgctg
    20821 cctgcgtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgtacg cagagcacca
    20881 acgggcgacg ctctttcagc acaacatctg ccgcacgggt cagtaagcca tcagtatagc
    20941 tatggacaat gccggacagg gttttgattg aacagggtaa aatcaccatc cccagtgtct
    21001 ggaaagaacc ggaagaaatg ctggcagcaa tatcgcgcgc atcgtgcgtc acatcggcta
    21061 atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg cgcgcggcct
    21121 ggctcatcac cagatgcgtt tcaacatctg cgacatcgcg cagaacctgt aataagcgca
    21181 cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc ataaatagtt
    21241 gccctgtcag acttgcaggc agactttgca ggatttcagc aggagttgca agtcagggtg
    21301 ccagaccggc accctgagcg aaggcatcat ccttcgttat gcatttcgag attttccact
    21361 tcgttctgac gttgcacggc tttggcgtca tcattacgta aagtatcaag gaaatcgagg
    21421 tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca ttcaaactgc
    21481 tggatatccg ggttttcagc gcgaacggct tcgatcagat cgttcagatc ctggaaaatc
    21541 aacccgtcag caccgatgat ctggcgaatt tcatctactt cgcgaccgtg agcgatcagt
    21601 tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc cggtgccgca
    21661 gaagcgagat acactttctt cgctccggct tcgcgtgcca tctcgataat ctgctcagaa
    21721 gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt ttttatcgcg gaactcggcg
    21781 cggttggcgt tcagtttacg gcgcacggac ttacgacgca gctgctgacc cggcatgatg
    21841 aaggtacggc ctacatagcg gtttttaacg aagccctgac ggtacggttt gcccagaata
    21901 cgcgcgattt ccagcgcgat atcacaagag gtttccggga tcgggatcac cacgtcgata
    21961 tccagatctt cccattcgcg ggcaattttc tcgcccagtt tcgtgcccat attcacgcgg
    22021 gcgctgtaaa cggaaatttt gtcgatgaac gagtccgggc gagcaaagta tacatactca
    22081 aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa ctgcccttct
    22141 tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta
    22201 tcgagcgcaa cgctttcgga agcgaccata tattctgtgc ggttctcgtc aatatcacgt
    22261 tttcccagta cgagcggacg aatcccgttt ggatcgcgga aagcaaccat accatggccg
    22321 ataatcatcg ccacgcaggc atacgcgccg cggattaagc ggtttgtggc agcaatggca
    22381 gcgaaaatat tgtcggcttc cagcgggtag tggcggaaat tgtccagttc gctggcaaag
    22441 atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac
    22501 agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag cgtaatgcca
    22561 tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc agccgtaggg
    22621 taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg agcttcaaat
    22681 acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc atctatggtg
    22741 atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac
    22801 tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct ttttcctcgt
    22861 taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct ttgcagataa
    22921 tcaaaaaacc atctgatgat aaaactgaac tgcgggatca gctgtgattt gctccagtct
    22981 tcgcttttcg acaccccggt aaaggagtcg agaaagaaga gaatggcggc aacaatcaac
    23041 acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga caaccccgtt
    23101 ttctccacca actggcctat cacgaagttc acgatagcac caacgatcag ggtagcgata
    23161 aacagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc cgtaaaccag
    23221 actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca tgtcaccagc
    23281 gataacgctt cacgaacaaa gccgcggatc aggctaacca gagaggaaaa agcaatcacc
    23341 gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc cctgtcatcc
    23401 tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc cttcccacgc
    23461 atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg cctttgcggc
    23521 atcggacaat gcgtgtcgga tgcggcgtga acgccttatt cgacctacgg ttctacccct
    23581 gcgcaggcct gataagacgc gccagcgtcg catcaggcaa gaccgtatta attcggcgta
    23641 tagcccatta ccacgccact taagccagaa agttgcttca actcacccag cgaacctttc
    23701 agcttatctt tcgaggcatc tggcccaacc agaatacggg taattttacc ctgcactggc
    23761 gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac aatctcattc
    23821 actttatcgg catttttcag cgcgcccagt tgcacaacat aggctttacc cgttggcgct
    23881 gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc ttcaaccttt
    23941 ggcttaagcg gctccaccgg tttccgcttc ggtggggcga ccggtgcagg ttccggttca
    24001 aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc ggcatcacct
    24061 gcccgtacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg ggtggcggct
    24121 ggcatcatat caggctcatc gcgatcgccc gcttttggca ccagcgggat agccgcaaac
    24181 tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat cacccccagc
    24241 gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac cgcttctcct
    24301 cgcgtcaatc acttccatga catgtgcgac cgtgtggaac gaaccacaca ccagcacggt
    24361 atcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgccacgc tatcaaatga
    24421 tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg gcccttccag
    24481 tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc cggcaatatc
    24541 tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta gcgctttcat
    24601 acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa taacgcgtgg
    24661 cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc cgtcgcgaat
    24721 ggcattttca ctgacctcca gcccgctggc acgcagtgcc gccagcgccg ttgcggcatt
    24781 cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat cgctaaacgc
    24841 ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta acagtgcact
    24901 tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga caattgccgg
    24961 tttttcgctg cggaagatgc ctgctttctc gcgaccaatg ctttcgcgat ctggccccag
    25021 ccagtcggta tgatccagcg caatgctggt tactaccgca acatcggcat cgacaatatt
    25081 ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa gttgtgcctg
    25141 tttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg aaatatcacc
    25201 gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca attcctgctc
    25261 ctgcacacgt acgcgctcgg tataacgcac cagatgcggt gaactgtaga cgcccacttt
    25321 gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt tgccattcgt
    25381 acccgcaacg gtaaacacaa atggcgctgg tttcagaacg ccaagacgcg ccgcgacctg
    25441 gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca gataagaaag
    25501 ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg tatccgctga
    25561 ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta tcagttatca
    25621 ggcctcaggt tcctgatctg gtaccggtgg caccactacg ccttcacgcg gcgcttcagg
    25681 attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca ggcgcatttc
    25741 cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac tgcgctggaa
    25801 tcctggcgga agtttttcgc gaacggtctg ttcgataaca cgcggaccgg caaagccgat
    25861 taacgctttc ggttcagcga tgttgagatc gcccagcatg gcgaaacttg cagaaacacc
    25921 acccatggtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct gcatttttgc
    25981 cagcgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct gcatacgtgc
    26041 gccaccagag gcggagaagc agatcagtgg gcagttatct tccagcgcct gctcaacggc
    26101 acgcacgaaa cgtgcaccta ccacagaccc catggaaccg cccataaagg cgaattcgaa
    26161 tgccgcagcg acaactggca ttccatacag ggtgcctttc atcaccacca gcgcatcttt
    26221 ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg agtcacgaaa
    26281 cttcagcaca tctttcggct caagctcgct acccagctcc acaaggcttc cttcatctaa
    26341 caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact tcggacagac
    26401 ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat cacacttagt
    26461 ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt taattcgttc
    26521 aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt ttatctttgg
    26581 ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt aaaacataac
    26641 agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta ttttgcggcg
    26701 cgtgccgcag cgcgtttgtg gcgaataaat tcgaaaacac ctggcaatat tgaaacaaaa
    26761 ataatgccaa caatcatcag tttaaggttg ctctgaatga aggggagcgt tccgaagaaa
    26821 taacccgcgt aggtaaaaat cagtacccat agcaacgcgc cgatgacgtt aaaagcggcg
    26881 aaatgacgat acgacatgtg gcccattccc gcaacgaacg gcgcgaacgt tctgacgatc
    26941 ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa ctgatgagtc
    27001 ttatcaagat aactgcgacg aaagattttc gaatccggat tactgaacag tttgtcacca
    27061 aataaccgtc caatggtgta gttcaccgcg tcacccacaa tcgcggcgat cagcatcagc
    27121 accaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc accggcaaca
    27181 aacagcaatg aatcacccgg taaaaacggc gttaccacca gaccggtttc acagaacaaa
    27241 attaagaaca aaatggcata aacccagacg ccatactccg cgaccagttc tgccagatgc
    27301 acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt agcctatgcc
    27361 ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat gttccgagca
    27421 ttattcttgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa gatcataccg
    27481 gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg ctgccgccag
    27541 cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt tgtgggcacc
    27601 gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg cattcgcttt
    27661 gatatctacc accacataag gaccgtgacg cgtgacatta atatgcataa cgttgcgcca
    27721 cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa
    27781 gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat gggttacccc
    27841 tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat aacggcgagc
    27901 cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac gcacggcgat
    27961 gtcaccaggt aaattcgcat ttacgcccaa cgtccacgca gcgtctttgc gctgcgcggt
    28021 cgtttcgaaa tgcacaacct gcccggtgcc gtgtacacca gcgtcagtac gcccggcgca
    28081 gaagacggtg atcggttcgt tcgccacctg ggagagcgcc ttttccagct tctcctgcac
    28141 actgcggact tcgttctgcc gttgccagcc gtaatactta ctgccgtcgt actcaatgcc
    28201 cagcgcaatt ttataaactg gcggttgttg ctggtcggac attagtacag atactcctgc
    28261 accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac gttatcggcc
    28321 accgaccaga actgaacttg ctccggcata ccgtagtcat tacgtacgca gccaacggaa
    28381 agatgcggcg taccagaagc atcccccacc tgagtcggga attcgttctc ttcagagagc
    28441 acaatatctt cgccctgagc aaacgcatca cgcgcttctt ctgccgccag cggacgcaga
    28501 gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac gacgcttgcc
    28561 gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct
    28621 tcacgcacgc taccttcgct atccggcagt aacggcagca tgttgaacgc cagctgacgc
    28681 ccgaagaaat cttcttcatc aatcggaatg ccgttgagca atttcgcact ctgccccgct
    28741 aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt aacgctgata
    28801 cgcgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagttg gctggtcaga
    28861 ctgtccggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa cgggtttact
    28921 tccggcacca ccagcggtac gtcaggttcg agagcaaaca atccgctgct atcgatcacc
    28981 aggcaacctg agttggtcgc ttcttcaacc caggcaccgg tagcttcttt gcctgcgaca
    29041 aaaaacgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac ggtgattgtc
    29101 ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc ataaatttcc
    29161 ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccac agcgccagtt
    29221 gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag aaatagcaaa
    29281 gctccctgcc aatattacag cagagagcat gaagaagaga ttaacgtgcc ggatgatgaa
    29341 cggcgttaaa acccagttta cacagcaatg atgccgcgct ggcgtcatca caaattacat
    29401 acagagatga ccattcacgg cgctcaagat agtttttgcg cagcttatcg aactcacccg
    29461 gtattccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac accaaatgca
    29521 ccagcctttt cagcgtcggt tgatcgagcg ggccatgcag cgtaatgcgg ccaaactctg
    29581 gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg cccaataaac ttgctataag
    29641 cttcaaacac ttgcgtagta ccgcgtgctt taccttccag ggtatagcct gcgatatgcg
    29701 gcgtgccgat atccactttt ttcagcaatt ccacgttgag ttccggttcg ccttcccaga
    29761 catccagcac tacgcttaac ttctggcctt cgttcagaca ggtcaatagg gcagtattat
    29821 cgacgactgc gccacggcag gcgttaatca gaatcgctcc tggtttcagg ctacggatca
    29881 gtttttcatc cgccagatgc agcgttttgt acggcccgtc tttgaagagt ggcgtatgga
    29941 aagtcagaat atccgcatgc tgaactaatt catccagcga gcggaaatcc ccctcatccc
    30001 cacggtcggc gcgaggcgga tcgcaaagca aggttgtaat ccctaacgct tccagtcgcg
    30061 cctgtaaacg gcgaccaacg ttaccaacgc ccacgatccc cactgtacgg tcgtgcagtg
    30121 aaaatccatc gcgttcggca agcatcagca gtgaggagaa aacatattcc accaccgcaa
    30181 tcgcattaca gcctggtgcc gcggaaaaac caattcccgc ctgctttaac cacgcttcat
    30241 cgacatggtc ggtccccgcg gtggcagtgc caacaaattt aatgggtttt cctcccagca
    30301 aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct gccagttgag
    30361 ccacggggat tggacgcccg ggaaccgcgg tcacctcacc caaacggcta aataagtcgc
    30421 gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct gtatgagacg
    30481 agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg aagatttcag
    30541 gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca accaccaggt
    30601 cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca gcaacgtact
    30661 tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacagcaaag agcagaactg
    30721 tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct ttcgcgtcac
    30781 ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa aagccattct
    30841 gcccgccagc ttttagcgca attaggggag tatctacgtc tggggaataa tcgtcaggcg
    30901 gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct ctctccggag
    30961 caattaaaga ccattctcac cctgttgcag gaagggaaga tggttattcc gcaacctcag
    31021 cagcgcgagg cgaccgaccg tccgttatta ccggcggagc acaatgcgct caaacagctg
    31081 gtgaccaaac ttgcggcggc aacgggagaa cccagcaagc agatctggca atcgatgctg
    31141 gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa ccatctggtg
    31201 acctggctac aggcgcgtca gacgctaagc cagcaaaata cgccgacgct tgaatcacta
    31261 cagatggcgc tgaaacaacc tttagatgcc agtgaactgg cggcgttatc ggcatatatc
    31321 cagcaaaaat atggtctttc tgcgcaatca tcgctttctt cagcccaggc cgaggatatt
    31381 cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat gcaaccgctg
    31441 cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg tcccgcgctg
    31501 tggatactgt tagtcgcgat tatcctgacg ctggtctggc tggttcgtta accccgacga
    31561 aatgacagta tcgtgacaat aattcccagc accgcagaga tcgccccggc aagaaatacc
    31621 gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac gccgagggcg
    31681 atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga cggtacgcgt
    31741 ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc ggttaacgcc
    31801 gcgcccgcta atgcgaccca cgcacctggg gcttgccaga gcagcaacaa gcccaccgtt
    31861 tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg catccagcca
    31921 aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa gcccgccatc
    31981 gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc aaaaccaacg
    32041 ccttgtagtg ccagacctaa cccaggtttc cagataagcc cgacaacact ccacagcgat
    32101 ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc ccacgccagt
    32161 aagggtaatg ccattgtggt gagcgccagt gcggcaaaac cgtaatggct atgaatcaac
    32221 aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg ccattccgtt ccatgacatc
    32281 actttgccgg aatgttttgg cccaacgatg cctaatcccc aggtcagagc gcccgtcagt
    32341 aactggcttt caccaaagcc aagaatcaaa cgcccgacga ccaacagggc aaatttgaac
    32401 ggtgcggaga cgggcaaaat cgccgccaac agcagcgcgc cgccagccag accacaagct
    32461 aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg cccggcgtaa
    32521 ccacgcgtca gcaccgtagc cagaaactga atgccgacgg caataccgac catggtgttg
    32581 ccatagccca gttcatgatg aacaaacagc gggataaccg gcaacggcag ccctacggtc
    32641 atgtaggtga gaaaaaccgc aaaagcgatg cggaagagtg aaaaattggc agaagatcgt
    32701 gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa ggcgaggagt
    32761 atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt taacgcgtac
    32821 gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaacaggcg gcgattgtca
    32881 atgatgtgaa aaagggaacc atcgggttcc cttttgcatt agtgccggag gcggtattgg
    32941 tgcatatcgt cggatgcgac gctggcgcgt cttatccgac ctactccgag ctactccgac
    33001 ctactgcgaa ttaatctttc agcttacgca ttaccagcgt ggcgttggtg ccgccgaagc
    33061 cgaagctgtt agacatcacg gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca
    33121 gacccgcagc ctgttcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt
    33181 ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg
    33241 tcattgcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagccg
    33301 ccagctcttt cacgtcgcca accggagtcg aagtaccgtg ggagttcagg tagtcgattg
    33361 gggtatcaac accgtgcatc gccatcttca tgcaacgcac tgcgccttcg ccagacggag
    33421 caaccatgtc tgcgccatca gacgttgcgc cgtagccaac gatttcagcg tagatatgag
    33481 caccacgtgc caacgcgtgt tccagttctt cgaccaccac cataccgccg ccgccagcga
    33541 taacgaaacc gtcacggtga gcgtcataag tacgggaggc tttttccggg gtgtcgttgt
    33601 atttggtaga cagcgcgccc attgcgtcga attcgcaggc catttcccag cacagctctt
    33661 cgccgccgcc agcaaacacg atatcctgtt tgcccagttg gatctgctct actgcgttac
    33721 cgatacagtg cgcggaagtc gcacatgcgg agctgatgga gtagttaacg ccgtgaatct
    33781 taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg accacatacg
    33841 ggcctaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctgaaaac
    33901 gcggggaacc gccgccggaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt
    33961 ccggggagag gccagcatct gcaattgcct gctccataga aaggaatgca taaatggatg
    34021 cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt
    34081 tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg
    34141 aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa
    34201 tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt
    34261 aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccaattaa
    34321 tagagaaatt tgcgcagcct gacacacatc gctaagatcg aggcaccgcc tgtaagacga
    34381 gtagcttacg tgaaacacaa cgccatacaa cctgccaacc tcgaatttaa tgctgagggt
    34441 acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg actggaagag
    34501 acacgctatg tttttctcgg aggtaaccaa ttagaggcac gctttcctga gcatccacat
    34561 cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg
    34621 caggcatttg ttcagtttcg cgaagcacat ccgcaagcgc aattacaacg cttacatttc
    34681 atcagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg
    34741 ccggaactgg caccgtgggc agaaaaactt caggcgcagt ggcctttgcc cttgcccggt
    34801 tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt
    34861 aatgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg
    34921 gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt taacgccatg
    34981 gcaaggttgg cgcgtccggg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc
    35041 cgtggtttac aggaagccgg attcacgatg
//
LOCUS       DAYPHN010000033        34026 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000033 DAYPHN010000000
VERSION     DAYPHN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.033"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            147..3890
                     /gene="narG"
                     /locus_tag="ACG0BW_001567"
     CDS             147..3890
                     /gene="narG"
                     /locus_tag="ACG0BW_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFK7258547.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     ILLHKLPVKRLQLADGRTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TADELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAKKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            3887..5425
                     /gene="narH"
                     /locus_tag="ACG0BW_001568"
     CDS             3887..5425
                     /gene="narH"
                     /locus_tag="ACG0BW_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFK7258548.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            5422..6132
                     /gene="narJ"
                     /locus_tag="ACG0BW_001569"
     CDS             5422..6132
                     /gene="narJ"
                     /locus_tag="ACG0BW_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFK7258549.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            6132..6809
                     /gene="narI"
                     /locus_tag="ACG0BW_001570"
     CDS             6132..6809
                     /gene="narI"
                     /locus_tag="ACG0BW_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFK7258550.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(6993..7124)
                     /locus_tag="ACG0BW_001571"
     ncRNA           complement(6993..7124)
                     /ncRNA_class="other"
                     /locus_tag="ACG0BW_001571"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7172..7303)
                     /locus_tag="ACG0BW_001572"
     ncRNA           complement(7172..7303)
                     /ncRNA_class="other"
                     /locus_tag="ACG0BW_001572"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7350..7434)
                     /locus_tag="ACG0BW_001573"
     tRNA            complement(7350..7434)
                     /locus_tag="ACG0BW_001573"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7398..7400),aa:Tyr,seq:gta)
     gene            complement(7469..7553)
                     /locus_tag="ACG0BW_001574"
     tRNA            complement(7469..7553)
                     /locus_tag="ACG0BW_001574"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7517..7519),aa:Tyr,seq:gta)
     gene            complement(7713..8555)
                     /gene="purU"
                     /locus_tag="ACG0BW_001575"
     CDS             complement(7713..8555)
                     /gene="purU"
                     /locus_tag="ACG0BW_001575"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFK7258551.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(8605..9063)
                     /gene="ychJ"
                     /locus_tag="ACG0BW_001576"
     CDS             complement(8605..9063)
                     /gene="ychJ"
                     /locus_tag="ACG0BW_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFK7258552.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9176..10081
                     /gene="rssA"
                     /locus_tag="ACG0BW_001577"
     CDS             9176..10081
                     /gene="rssA"
                     /locus_tag="ACG0BW_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFK7258553.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10173..11186
                     /gene="rssB"
                     /locus_tag="ACG0BW_001578"
     CDS             10173..11186
                     /gene="rssB"
                     /locus_tag="ACG0BW_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFK7258554.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            11388..12296
                     /gene="galU"
                     /locus_tag="ACG0BW_001579"
     CDS             11388..12296
                     /gene="galU"
                     /locus_tag="ACG0BW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFK7258555.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(12441..12854)
                     /gene="hns"
                     /locus_tag="ACG0BW_001580"
     CDS             complement(12441..12854)
                     /gene="hns"
                     /locus_tag="ACG0BW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFK7258556.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            13458..14075
                     /gene="tdk"
                     /locus_tag="ACG0BW_001581"
     CDS             13458..14075
                     /gene="tdk"
                     /locus_tag="ACG0BW_001581"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFK7258557.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(14378..17053)
                     /gene="adhE"
                     /locus_tag="ACG0BW_001582"
                     /gene_synonym="adhC"
     CDS             complement(14378..17053)
                     /gene="adhE"
                     /locus_tag="ACG0BW_001582"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFK7258558.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            17446..17499
                     /gene="ychE"
                     /locus_tag="ACG0BW_001583"
     CDS             17446..17499
                     /gene="ychE"
                     /locus_tag="ACG0BW_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFK7258559.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            17530..18177
                     /gene="ychE"
                     /locus_tag="ACG0BW_001584"
     CDS             17530..18177
                     /gene="ychE"
                     /locus_tag="ACG0BW_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFK7258560.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(18335..18631)
                     /locus_tag="ACG0BW_001585"
     CDS             complement(18335..18631)
                     /locus_tag="ACG0BW_001585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258561.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLLIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKSSGITNIISIILQQAQDYDVAKIT"
     gene            18915..20546
                     /gene="oppA"
                     /locus_tag="ACG0BW_001586"
     CDS             18915..20546
                     /gene="oppA"
                     /locus_tag="ACG0BW_001586"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFK7258562.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            20632..21552
                     /gene="oppB"
                     /locus_tag="ACG0BW_001587"
     CDS             20632..21552
                     /gene="oppB"
                     /locus_tag="ACG0BW_001587"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFK7258563.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            21567..22475
                     /gene="oppC"
                     /locus_tag="ACG0BW_001588"
     CDS             21567..22475
                     /gene="oppC"
                     /locus_tag="ACG0BW_001588"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFK7258564.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            22487..23500
                     /gene="oppD"
                     /locus_tag="ACG0BW_001589"
     CDS             22487..23500
                     /gene="oppD"
                     /locus_tag="ACG0BW_001589"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFK7258565.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            23497..24501
                     /gene="oppF"
                     /locus_tag="ACG0BW_001590"
     CDS             23497..24501
                     /gene="oppF"
                     /locus_tag="ACG0BW_001590"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFK7258566.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(24554..24883)
                     /gene="yciU"
                     /locus_tag="ACG0BW_001591"
     CDS             complement(24554..24883)
                     /gene="yciU"
                     /locus_tag="ACG0BW_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFK7258567.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(24918..26378)
                     /gene="cls"
                     /locus_tag="ACG0BW_001592"
     CDS             complement(24918..26378)
                     /gene="cls"
                     /locus_tag="ACG0BW_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFK7258568.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            26521..26694
                     /gene="yciY"
                     /locus_tag="ACG0BW_001593"
     CDS             26521..26694
                     /gene="yciY"
                     /locus_tag="ACG0BW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFK7258569.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(26749..28002)
                     /gene="kch"
                     /locus_tag="ACG0BW_001594"
     CDS             complement(26749..28002)
                     /gene="kch"
                     /locus_tag="ACG0BW_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFK7258570.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADNKESAQK"
     gene            complement(28303..28599)
                     /gene="yciI"
                     /locus_tag="ACG0BW_001595"
     CDS             complement(28303..28599)
                     /gene="yciI"
                     /locus_tag="ACG0BW_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFK7258571.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            28823..29542
                     /gene="tonB"
                     /locus_tag="ACG0BW_001596"
     CDS             28823..29542
                     /gene="tonB"
                     /locus_tag="ACG0BW_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFK7258572.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(29582..29980)
                     /gene="yciA"
                     /locus_tag="ACG0BW_001597"
     CDS             complement(29582..29980)
                     /gene="yciA"
                     /locus_tag="ACG0BW_001597"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFK7258573.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(30085..30624)
                     /gene="yciB"
                     /locus_tag="ACG0BW_001598"
     CDS             complement(30085..30624)
                     /gene="yciB"
                     /locus_tag="ACG0BW_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFK7258574.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(30654..31397)
                     /gene="yciC"
                     /locus_tag="ACG0BW_001599"
     CDS             complement(30654..31397)
                     /gene="yciC"
                     /locus_tag="ACG0BW_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFK7258575.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(31425..31514)
                     /locus_tag="ACG0BW_001600"
                     /pseudo
     CDS             complement(31425..31514)
                     /locus_tag="ACG0BW_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            31754..32392
                     /gene="ompW"
                     /locus_tag="ACG0BW_001601"
     CDS             31754..32392
                     /gene="ompW"
                     /locus_tag="ACG0BW_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFK7258576.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(32452..32958)
                     /gene="yciE"
                     /locus_tag="ACG0BW_001602"
     CDS             complement(32452..32958)
                     /gene="yciE"
                     /locus_tag="ACG0BW_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFK7258577.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIIQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(33004..33504)
                     /gene="yciF"
                     /locus_tag="ACG0BW_001603"
     CDS             complement(33004..33504)
                     /gene="yciF"
                     /locus_tag="ACG0BW_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFK7258578.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(33590..33769)
                     /gene="yciG"
                     /locus_tag="ACG0BW_001604"
     CDS             complement(33590..33769)
                     /gene="yciG"
                     /locus_tag="ACG0BW_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFK7258579.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         8684 a         8031 c         8622 g         8689 t
ORIGIN      
        1 ggtatcttag gaatttactt tatttttcat ccccatcact cttgatcgtt atcaattctc
       61 acgctgtttc agagcgttac cttgccctta aacattagca atgtcgattt atcagagggc
      121 cgacaggctc ccacaggaga aaaccgatga gtaaattcct ggaccggttt cgctacttca
      181 aacagaaggg tgaaaccttt gccgatgggc atggccagct tctcaatacc aaccgtgact
      241 gggaggatgg atatcgccag cgttggcagc atgacaaaat cgtccgctct acccacgggg
      301 taaactgcac cggctcctgc agctggaaaa tctacgtcaa aaacggtctg gtgacctggg
      361 aaacccagca gactgactat ccgcgtactc gcccggacct gccaaaccat gaacctcgcg
      421 gctgcccgcg cggtgccagc tactcctggt atctttacag cgccaaccgc ctgaaatacc
      481 cgatgatgcg caaacgcctg atgaaaatgt ggcgtgaagc gaaagcgctg catagcgatc
      541 cagttgaggc atgggcttct atcattgaag acgccgataa agcgaaaagc tttaagcagg
      601 cgcgtggacg cggtggtttt gttcgttctt cctggcagga agtgaatgaa ctgatcgccg
      661 cctctaacgt ttacaccatc aaaaactacg gcccggaccg cgtcgctggc ttctcgccaa
      721 ttccggcaat gtcgatggtt tcttacgctt ccggcgcgcg ttatctctcg ctgcttggcg
      781 gtacttgctt aagcttctac gactggtact gcgacctgcc tcctgcgtct ccacaaacct
      841 ggggcgagca gactgacgta ccggaatctg ctgactggta caactccagc tacatcatcg
      901 cctggggttc aaacgtgccg cagacgcgta ccccggatgc tcacttcttt actgaagtgc
      961 gttacaaagg caccaaaact gttgccgtca cgccagacta cgctgaaatc gccaaactgt
     1021 gcgatctgtg gttggcaccg aaacagggca ccgatgcggc aatggcgctg gcgatgggcc
     1081 acgtaatgct gcgtgaattc cacctcgaca acccaagcca gtatttcacc gactatgtgc
     1141 gtcgctacac cgacatgccg atgctggtga tgctggaaga gcgcgacggt tactacgctg
     1201 cgggtcgtat gctgcgcgct gccgatctgg tggatgcgct gggccaggaa aacaatccgg
     1261 aatggaaaac agtcgccttt aataccaatg gcgaaatggt tgcgccgaac ggttctattg
     1321 gcttccgctg gggcgagaag ggcaaatgga atcttgagca gcgcgacggc aaaactggcg
     1381 aagaaaccga gctgcaactg agcctgctgg gtagccagga tgagatcgct gaggtgggct
     1441 tcccgtactt tggtggcgac ggcactgaac acttcaacaa agtggaactg gaaaacattc
     1501 tgctgcacaa gctgccggtg aaacgcctgc aactggctga tggccgcacc gctctggtga
     1561 ccaccgttta cgatctgacg ctggcaaact atggtctgga acgtggcctg aacgacgtta
     1621 actgcgcaac cagctacgac gatgtgaaag cttacacccc tgcgtgggcc gagcagatta
     1681 ccggcgtttc tcgcagccag attattcgca tcgcccgtga atttgccgat aacgcggata
     1741 aaacgcacgg tcgttcgatg attatcgtcg gtgcggggct gaaccactgg tatcacctcg
     1801 atatgaacta tcgtggtctg atcaacatgc tgattttctg cggctgtgtc ggtcagagcg
     1861 gggggggctg ggcgcactat gtaggtcagg aaaaactgcg tccgcaaacc ggctggcagc
     1921 cgctggcgtt tgccctcgac tggcagcgtc cggcgcgtca catgaacagc acctcttatt
     1981 tttataacca ctccagccag tggcgttatg aaaccgtgac cgcagacgag ttgctgtcgc
     2041 cgatggcgga taaatcccgt tataccggac acttgatcga ctttaacgtc cgtgcagaac
     2101 gtatgggctg gctgccgtct gcgccgcagt taggcactaa cccgctaact atcgctggcg
     2161 aagcgaaaaa agcgggcatg aatccggtgg attacaccgt gaaatccctg aaagagggtt
     2221 ctatccgttt tgcggcagaa caaccagaaa acggtaaaaa ccacccgcgt aacctgttca
     2281 tctggcgttc taacctgctc ggttcttccg gtaaaggtca tgagtttatg ctcaagtacc
     2341 tgctggggac ggagcacggt atccagggca aagacctggg gcagcagggc ggcgtgaagc
     2401 cggaagaagt ggactggcag gacaacggtc tggaaggcaa gctggatctg gtggtgacgc
     2461 tggacttccg tctgtcgagc acctgtctct attccgacat cattttgccg acggcgacct
     2521 ggtacgaaaa agacgacatg aatacttcgg atatgcatcc gtttattcac ccgctgtctg
     2581 cggcggtcga tccggcctgg gaagcgaaaa gcgactggga aatctacaaa gccatcgcga
     2641 agaaattctc cgaagtgtgc gttggtcatc tgggtaaaga aactgacatc gtcacgctgc
     2701 ctatccagca cgactctgcc gctgaactgg cgcaaccgct ggatgtgaaa gactggaaaa
     2761 aaggcgaatg cgacctgatc ccaggtaaaa ctgcaccgca cattatggtc gtagagcgcg
     2821 attatccggc aacttacgaa cgctttacct ctatcggccc gctgatggag aaaatcggta
     2881 acggcggtaa agggattgcc tggaacaccc agagcgagat ggatctgctg cgtaagctca
     2941 actacaccaa agcggaaggt ccggcaaaag gccagcccat gctgaacacc gcaattgatg
     3001 cggcagagat gatcctgacg ctggcaccgg aaaccaacgg tcaggtagcc gtgaaagcct
     3061 gggctgccct gagcgagttt accggtcgtg accatacgca tctggcgctg aataaagaag
     3121 acgagaagat tcgcttccgc gatattcagg cacagccgcg caaaatcatc tccagcccga
     3181 cctggtctgg ccttgaagat gaacacgttt cttacaacgc cggttacacc aacgttcacg
     3241 agctgatccc atggcgtacg ctctctggcc gtcagcagct gtatcaggat caccagtgga
     3301 tgcgtgattt cggtgaaagc ctgctggttt atcgcccgcc aatcgacacc cgttcggtaa
     3361 aagaggtgat gggccagaaa tctaacggta acccggaaaa agcgctcaac ttcctgacgc
     3421 cgcaccagaa gtggggtatc cactccacct acagcgacaa cctgctgatg ctgactttag
     3481 gtcgcggtgg tccggtggtc tggttgagtg aagccgatgc caaagagctg ggtatcgccg
     3541 ataacgactg gattgaagtc ttcaacagca acggtgcact gactgcccgt gcggttgtca
     3601 gccagcgtgt tccggcaggg atgaccatga tgtaccacgc gcaggaacgt atcgttaacc
     3661 tgccgggttc ggaaattacc caacagcgtg gtggtatcca taactcggtc acccgtatca
     3721 cgccgaaacc gacgcatatg attggcggct atgcccatct ggcatacggc tttaactact
     3781 acggcaccgt aggttctaac cgcgatgagt ttgttgtggt gcgtaagatg aagaacattg
     3841 actggttaga tggcgaaggc aatgaccagg tacaggagag cgtaaaatga aaattcgttc
     3901 acaagtcggc atggtgctga atctcgataa gtgcatcggc tgccacacct gttcagttac
     3961 ctgtaaaaac gtctggacca gccgcgaagg cgtggaatac gcgtggttca acaacgtgga
     4021 aaccaagccg ggccagggct tcccgactga ctgggaaaac caggaaaaat acaaaggcgg
     4081 ctggattcgt aaaatcaacg gcaaactgca gccgcgcatg ggtaaccgtg ccatgctgct
     4141 gggtaaaatc ttcgctaacc cgcatctgcc ggggatcgac gattattacg agccgttcga
     4201 ttttgactat cagaacctgc ataccgcgcc ggaaggcagc aaatcgcagc cgattgcccg
     4261 tccgcgttcg ctgattaccg gggaacggat ggcgaaaatc gaaaaagggc cgaactggga
     4321 agatgacctg ggcggtgagt ttgacaaact ggcgaaagac aagaacttcg acaacatcca
     4381 gaaggcgatg tatagccagt tcgaaaacac cttcatgatg tatttgccgc gcctgtgcga
     4441 acactgcctg aacccggcat gtgtggcgac ctgcccgagc ggtgcgattt acaagcgtga
     4501 agaagatggc atcgtcctga tcgaccagga taaatgccgt ggctggcgta tgtgcatcac
     4561 cggatgccca tacaaaaaaa tctacttcaa ctggaagagc ggtaagtctg agaagtgcat
     4621 cttctgctat ccgcgtattg aagcgggtca gccgacggtg tgctctgaaa cctgtgtcgg
     4681 tcgtatccgt tatctcggcg tgctgctgta cgatgcggac gccattgaac gtgcagccag
     4741 caccgagaac gagaaagatc tctaccagcg tcagctggag gtgttcctcg atccgaacga
     4801 tccgaaagtc atcgagcagg caattaaaga cggtattccg ctgagcgtta ttgaagccgc
     4861 acagcagtcg ccggtttata aaatggcgat ggaatggaaa ctggcgctgc cgctgcatcc
     4921 agaatatcgc acactgccga tggtctggta cgtgccgcct ctgtcgccga ttcagtctgc
     4981 agcagacgcg ggtgagctgg gcagcaacgg cattctgcca gacgtcgaaa gcctgcgtat
     5041 tccggtacag tatctggcga atctgctgac cgccggtgat accaaaccgg tactgcgcgc
     5101 gctgaaacgt atgctggcga tgcgtcatta caaacgtgct gaaaccgttg acggtaaagt
     5161 cgatacccgt gcgctggaag aggtcggtct gaccgaagcc caggcacagg agatgtaccg
     5221 ttatctggcg attgctaact acgaagatcg ctttgtggtg ccgagcagtc atcgtgaact
     5281 ggcgcgggaa gccttcccgg agaaaaatgg ctgcggcttt acctttggtg atggctgcca
     5341 cggttcagat accaaattca atctgttcaa cagccgccgt atcgatgcca tcgacgtaac
     5401 cagcaaaacg gagccgcacc catgatcgaa ctcgtgattg tatcgcgtct ccttgaatat
     5461 ccggatgctg ctttatggca gcatcaacaa gagatgtttg aggcaattgc tgcgtcgaaa
     5521 aatctgtcga aagaagatgc ccatgcgctg ggcattttcc tgcgcgattt aacgacgatg
     5581 gatccgctcg atgcccaggc gcagtacagc gaactgttcg atcgtggtcg cgccacgtcg
     5641 ctgttgctgt ttgaacacgt tcacggggaa tcccgcgatc gcggtcaggc gatggtggac
     5701 ctgctggcac agtacgagca gcacggtttg cagttaaaca gccgcgaatt gccggaccac
     5761 ctgccgctgt atctggagta tctggcgcag ctgccgcaaa gcgaagccgt ggagggtttg
     5821 aaagatattg cgccgattct ggcattgctg agcgcgcgtc tgcaacagcg tgaaagccgt
     5881 tatgccgtgc tgtttgatct gctgctgaaa ctggccaata ccgctatcga cagtgacaaa
     5941 gtggcggaaa aaattgccga cgaagcgcgc gacgatacgc cgcaggcgct ggatgctgtc
     6001 tgggaagaag agcaggttaa attctttgct gacaaaggct gcggtgattc agcaatcact
     6061 gctcatcagc gtcgctttgc cggtgccgtc gcgccgcaat atctgaatat caccaccgga
     6121 ggacagcact aatgcaattc ctgaatatgt tcttctttga tatctacccg tacatagccg
     6181 gggcggtctt cctgattggt agctggctgc gttatgacta cgggcagtac acctggcgcg
     6241 cggcgtccag ccagatgctg gatcgcaaag ggatgaacct ggcgtcgaac ctgttccata
     6301 tcggtattct ggggattttt gtcggtcact tcttcggtat gctgacgccg cactggatgt
     6361 atgaagcctg gctgccgatt gaagtgaaac aaaaaatggc gatgtttgct ggtggtgcca
     6421 gcggcgtgct gtgtctgatt ggcggcgtgc tgttgctgaa acgtcgtctg ttcagcccac
     6481 gcgtgcgtgc aacgactacc ggagcggata tcctgatcct gtcgctgctc gttatccagt
     6541 gcgcgctggg cttgctgacc attccgttct cagcccagca tatggacggt agcgagatga
     6601 tgaaactggt tggctgggcg cagtcggtag tgaccttcca cggtggtgct tctcaacacc
     6661 ttgatggtgt ggcatttatc ttccgtctgc acctggtgtt ggggatgacg ttattcctgc
     6721 tgttcccgtt ctcgcgtctg gtgcacatct ggagcgtacc ggtggagtat ctgacacgta
     6781 agtatcagct ggtgcgcgct cgtcactaag cgaattttag tttgcataga ccccgcttcg
     6841 gcggggtttt ttatgggcac ggtacggggt gagttgtcgg atgcgcttcg cttatccgac
     6901 ctacagggga ggatattgta ggtccggtaa acgcggtgcc attgggtaga attagctcgg
     6961 agtaacaggt tttgagtatt tacaggaagt gatggtggtg ggggaaggat tactcagcgc
     7021 tgcgcgcttc acccttcggg tcgttgcctg cggcaacgct ttctcgctgg cgctcgagtc
     7081 gcaccttggt cgaagcttct catccttccc cgcatgggca gaatatttaa ttgcggattc
     7141 gtttgagagt tcacggactt ttgaaaagtg atggtggtgg gggaaggatt actcagcgct
     7201 gcgcgcttcg cccttcgggt cgttgcctgc ggcaacgctc tctcgctggc gctcgagtcg
     7261 aaccttggtc gaagcttctc atccttcccc gcatgggcag aatatttaat cgcggattcg
     7321 tttgagagtt cacggacttt tgaaagtgat ggtggtgggg gaaggattcg aaccttcgaa
     7381 gtcgatgacg gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccaggggt
     7441 aattcaaatt ttgaggtaat gcttgagatg gtggtggggg aaggattcga accttcgaag
     7501 tcgatgacgg cagatttaca gtctgctccc tttggccgct cgggaacccc accacggggt
     7561 aatgcttttt actggcctgc tcccttatcg ggaagcgggg cgcatcatat caaatgacgc
     7621 gccgctgtaa agtgttacgt tgagaaaaat gaactggttg cgtaattttc acccgtaacg
     7681 gattaaaggt aaccagttat ttttgctggc gattaaagaa taatcgtccg attaccgtaa
     7741 acaaagacgc gctgcgccag tactttgtag agtgcgcgac ttaagacgtt tttctcgacg
     7801 tcacgacctg cgcgcatcat atcttcagct gtgtaggtat gatcgacatg aataacgtcc
     7861 tgcatgatga ttggaccttc gtccagattg tcattcacat agtgagcggt tgcgccaata
     7921 atcttcacgc cacgttcata ggcctggtga taaggacgtg cgccgataaa cgctggcagg
     7981 aatgaatggt gaatattgat gatcttattc gggaagcgtg ccacaaattc tggcgttaat
     8041 acccgcatat acttcgccag caccacgtag tcaggctgat aagcatcaat ggcatccgcc
     8101 atcttttgat cgtgctcgtt gcggcttaac ccttcatggc ttaccagctc aaacggaata
     8161 tcaaaacgct caaccagaga acgtaaagta tcgtggttac caataactgc cgcgatttcg
     8221 acatccaggc cgccgtaatt ggctttcatc aacaaatcgc caaggcaatg cgcctcttta
     8281 gtgaccagaa tcactatccg gcgacgacca gcaggattca gctcacgtac ggagccttct
     8341 ggcaatgcgc tgtcgagatc cgccagcagg gtggaatcat taaaaattcc ttccagttcc
     8401 gtgcgcataa aaaagcgccc ggtacggtga tcaacaaatt cattgttctg tacgatattt
     8461 aactcgtgct tgtagcaaat attggtaata cgtgcgatca gacctttttg gtccggacaa
     8521 atagtacgca gaactttacg ttggagtgaa tgcattgctg gaaaaacctt gttgagagtg
     8581 tttgctaaac cgtaccgtca accattattg gccgcagcac tttttaaatt ttttacctga
     8641 accacaaggg cagggatcgt tgcggccaaa ctgcggacgt gtaccgtcaa tatagtacca
     8701 ttgaccgttt tctttcaaaa atcgcgaacg ttcaatgatt gcacctgttt tgccaccttc
     8761 ggtaaatcgc gcaacaaaac tgacaaatcc gatattgtcg acgtcctgcc aacaatgttc
     8821 aaatacggtc aatcccaacc attcagtatg cgcaaatccg gcaatcaatt cggcacgtaa
     8881 cgccgccgct ccacaagatg gatgccaggt ctttattaaa taatctgcgt ctttcatcac
     8941 aaaagcgcag taacgcgaac gcatgagatg ttctggatca ggtgcaacct tttcaccaga
     9001 cacataaggg tggcaacata ggctatactc gacagcacta ccacagggac aaagctgaga
     9061 cacaaataat ctccctggaa acaataacgg cgtattaacc gcctgagtag cactatgtta
     9121 accgagcagt agcgatgttg ctacgattgc attccagggg aatcttgcgg gaataatgag
     9181 aaagataaaa atagggctgg cgctgggatc tggcgcggcg agaggttggt cgcatattgg
     9241 cgttattaat gcgctaaaaa aagtgggtat tgaaattgat atcgttgcag gatgttcaat
     9301 tggttcgctg gtgggcgctg cctatgcatg cgatcgacta tctgcgctgg aagattgggt
     9361 gacctctttc agttattggg atgttttacg cctgatggat ctctcctggc agcgcggtgg
     9421 gttactgcgc ggcgagcgtg tcttcaatca atatcgcgaa ataatgccgg aaacagagat
     9481 cgaaaattgt tcccgtcgct tcgcggctgt tgccaccaat ttaagtactg ggcgcgaatt
     9541 atggtttact gaaggcgatc tccatcttgc tattcgtgca tcatgcagta ttccaggact
     9601 aatggcaccc gttgcgcata acggctactg gctggttgat ggcgctgtag ttaacccaat
     9661 tcctatttcg ctcacgcgtg cattgggtgc tgatattgtg atagcggtcg acctgcagca
     9721 cgatgctcat ttgatgcaac aagatctgct ctcctttaat gtcagtgaag aaaatagcga
     9781 gaatggtgat tctctgccgt ggcatgcgcg tctgaaagaa aggttgggca gcataacgac
     9841 acgtcgggcg gtgacagcgc caacggcaac agagattatg accacttcta tccaggtgct
     9901 ggagaaccgc cttaaaagga accgcatggc aggtgatccg cccgatattc tgattcaacc
     9961 tgtttgcccg caaatatcta cgcttgattt ccatcgcgcg cacgctgcca ttgcggctgg
    10021 gcagctggca gtggaaaaga aaatggacga acttttgccg ttggtacgca ccaacatttg
    10081 accagaattt ttatctacac ttaagttaat tctgacaggc gcaggtggca atagcatgcc
    10141 actattgagt aaagccagtc aggggagaga acatgacgca gccattggtc ggaaaacaga
    10201 ttctcattgt tgaagatgag caggtatttc gctcgcttct ggattcgtgg ttttcctcat
    10261 tgggagcgac aacggtactg gcggctgatg gggtggatgc ccttgagttg ctgggaggtt
    10321 tcactccaga cctgatgata tgtgatatcg cgatgccacg aatgaacggg cttaaactgc
    10381 tggagcatat acgcaacaga ggtgaccaga cacctgttct cgtgatatct gccactgaaa
    10441 atatggcaga tattgccaaa gcgttacgtc tgggcgttga agatgttttg ctgaaaccag
    10501 ttaaagatct gaatcgcttg cgcgagatgg tttttgcctg tctctatccc agcatgttta
    10561 attcccgcgt tgaggaagag gagaggcttt ttcgcgactg ggatgcaatg gttgataacc
    10621 ctgccgcagc ggcgaaatta ttacaggaat tgcaaccgcc ggttcagcag gtgatttccc
    10681 attgccgggt taattatcgt cagttggttg ccgcggacaa acccggcctg gtgcttgata
    10741 ttgccgcact ttcggaaaac gatctggcat tttattgcct tgatgtcacc cgagctggac
    10801 ataatggcgt acttgctgcc ttgttattac gcgcattgtt taacggatta ttacaggaac
    10861 agcttgcaca tcaaaatcaa cggttgccag agttgggcgc gttattgaag caggtaaacc
    10921 atttactccg tcaggccaat ctgccggggc agtttccact attagttggc tattatcatc
    10981 gcgaactgaa aaatctcatt ctggtttctg cgggtctgaa tgcgacgtta aataccggcg
    11041 aacaccaggt gcaaatcagt aatggtgttc cgttaggcac tttaggtaac gcttatttga
    11101 atcaattgag ccagcgatgc gatgcctggc aatgccaaat atggggaacc ggtggtcggc
    11161 ttcgcttgat gttgtctgca gaatgagcaa acgataacgc gggctaaatt tgcattacca
    11221 gctaatgtcg gctggtggta ctatcgtcgc cattcgtata agtaattgtc ttaattatgc
    11281 taactcgcat ccttttcaga acttagcccc ttcggggtgc tgatatactg ggatgcgata
    11341 cagaaatatg aacacgttca aaacacgaac agtccaggag aatttaaatg gctgccatta
    11401 atacgaaagt caaaaaagcc gttatccccg ttgcgggatt aggaaccagg atgttgccgg
    11461 cgacgaaggc catcccgaaa gagatgctgc cacttgtcga taagccatta attcaatacg
    11521 tcgtgaatga atgtattgcg gctggcatta ctgaaattgt gctggttact cactcatcta
    11581 aaaactcgat tgaaaaccac tttgatacca gttttgaact ggaagcaatg ctggaaaaac
    11641 gtgtaaaacg tcaactgctt gatgaagtgc agtctatttg tccgccgcac gtgactatta
    11701 tgcaagttcg tcaggggctg gcgaaaggcc tgggacacgc ggtattgtgt gctcacccgg
    11761 tagtgggtga tgaacctgtg gctgttattt tgcctgacgt tattctggat gaatatgaat
    11821 ccgatttgtc acaggataac ctagcagaga tgatccgccg ctttgatgaa acgggtcata
    11881 gccagatcat ggttgaaccg gttgctgatg tgaccgcata tggcgttgtg gattgcaaag
    11941 gcgttgaatt agcgccgggt gaaagcgtac cgatggttgg cgtggttgaa aaaccaaaag
    12001 cggatgttgc gccgtctaat ctcgctattg tgggtcgtta cgtacttagc gcggatattt
    12061 ggccgttgct ggcaaaaacc cctccgggag ctggtgatga aattcagctc accgacgcaa
    12121 ttgatatgct gatcgaaaaa gaaacggtgg aagcctatca tatgaaaggg aagagccatg
    12181 actgcggtaa taaattaggt tacatgcagg ccttcgttga atacggtatt cgtcataaca
    12241 cccttggcac ggaatttaaa gcctggcttg aagaagagat gggcattaag aagtaacatc
    12301 cgtatcgatg ttatccacga aacggcgttg agcaatcgac gccgtttttt tatagcttat
    12361 tcttattaaa ttgtcttaaa ccggacaata aaaaatcccg ccgatggcgg gatttttaag
    12421 caagtgcaat ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc
    12481 tcatccattg cttttttgat tacagctgga gtacggccct ggccagtcca ggttttagtt
    12541 tcgccgtttt cgtcaacgta gctatatttt gccggacgct gagcacgttt agctttggtg
    12601 ccagatttaa cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc
    12661 agcatttcgc gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg
    12721 ctttcttctt cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc
    12781 gtttcaagtg tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta
    12841 agtgcttcgc tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa
    12901 tagagcctta aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt
    12961 attttgtgcc gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag
    13021 ccctatttgc gccgatgatt tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca
    13081 ttttatgggg agattatcgt aggctgacga cctttcagtc ttctgtatta gtcgtgttta
    13141 cgagaattcc gtattaagcg aatgatgaaa agtagaacag tcgcaataag agcagggact
    13201 tagtattgca ctatctccag gaggtcaaca gagggctatt acttgcgcaa caggttaaag
    13261 attgtgaata gttaccagca gtcatttacc cgcttataac aagcgaggca gttgtaatga
    13321 tagctcagaa ggattatgca aggcttcgta agggagaacg catataccac ttctgtgcat
    13381 actgttgagc tgaaaaactg acgaattatg ataaactcca gccaacttta attcatatca
    13441 ttgagggcct gtggctgatg gcacagctat atttctacta ttccgcaatg aatgcgggta
    13501 agtctacagc attgttgcaa tcttcataca attaccagga acgcggcatg cgcactgtcg
    13561 tatatacggc agaaattgat gatcgctttg gtgccgggaa agtcagctcg cgtataggtt
    13621 tgtcatcgcc tgcaaaatta tttaaccaaa attcatcatt atttgatgag attcgtgcgg
    13681 aacatgaaca gcaggcaatt cattgcgtac tggttgatga atgccagttt ttaaccagac
    13741 aacaagtata tgaattatcg gaggttgtcg atcaactcga tatacccgta ctttgttatg
    13801 gtttacgtac cgattttcga ggtgagttat ttattggcag ccaatactta ctggcatggt
    13861 ccgacaaact ggttgaatta aaaaccatct gtttttgtgg ccgcaaagca agcatggtgc
    13921 tgcgtcttga tcaagcaggc agaccttata acgaaggtga gcaggtggtt attggtggta
    13981 atgaacgcta cgtttctgta tgccgtaaac actataaaga agcgttagaa gtcggctcat
    14041 taacggctat tcaggaaagg catcgccacg attaataaga atttctttac tgacagggtg
    14101 agcagggcgc ttttatcctg tcagttcgtt ttactcactt catccggtgt atataccctt
    14161 ctcgacagtt ctttaacgcc actatacccc tcacagagcg cttttacagt gacgttgact
    14221 tgtgacctgt aaagtacaat atcgctgtgt gtaggcgtta tacatcgccg taaatatgat
    14281 gaaggcaaat gctgtcggtt tatgggaaag ttgctttggg taaaacaaaa aaacggcccc
    14341 agaagggacc gtttatgttg ccagacagcg ctactgatta agcggatttc ttcgcttttt
    14401 tctcagcttt agccggagcg gcttcttttt tcgctgcagt ttcaccttct acataatcac
    14461 gaccgtagta ggtatccagc agaatctgtt tcagctcgga gatcagtggg taacgcgggt
    14521 tagcgccggt gcactggtca tcgaatgcat cttcagacag tttatccaca ttcgccagga
    14581 agtctgcttc ctgaacgcca gcttcacgga tagatttcgg aatacccagt tcagctttca
    14641 gcgtttccag ccatgccagc agtttctcga tcttagcagc agtacggtcg cccggtgcgc
    14701 tcagacccaa gtggtcagca atttcagcat aacgacggcg agcctgcgga cggtcatact
    14761 ggctgaatgc agtctgcttg gtcgggttgt cgttcgcgtt gtagcgaata acgttacaaa
    14821 tcagcagggc gtttgccaga ccgtgcggaa tatggaactg ggaacccagt ttgtgcgcca
    14881 ttgagtgaca tacacccagg aaggcgttcg caaacgcgat acccgcgata gttgctgcac
    14941 tgtgaacacg ttcacgcgct accggatttt tagaaccttc gtggtaggac gctggcagat
    15001 attctttcag cagtttcagc gcctgcagag cctgaccatc agagaactca gatgccagta
    15061 cagaaacata agcttccatg gcgtgagtta ctgcgtccag accaccgaaa gcacacaggg
    15121 acttcggcat gtccataacc aggttggcat cgacaatcgc catatccggg gtcagcgcat
    15181 agtctgccag cggatatttc tgaccagtag cgtcgtcagt tacaaccgca aacggagtga
    15241 cttcagaacc tgtaccagaa gtggtggtga cagcgatcat tttcgctttc acgcccattt
    15301 tcgggaactt gtagatacgt ttacggatat ccataaagcg cagcgccagt tcttcgaagt
    15361 gagtttccgg atgttcgtac atgacccaca tgatcttcgc ggcgtccatc ggggaaccac
    15421 cacccagcgc gataatcacg tctggtttaa aggagtttgc cagttctgca cctttacgaa
    15481 cgatgctcag ggtcgggtca gcttctactt cgaagaagac ttcagtttca acgcctgctg
    15541 ctttcagtac ggaagtgatc tgatcagcat aaccattgtt gaacaggaag cggtcagtca
    15601 cgatgagcgc acgtttgtgg ccatcagtaa tcacttcatc cagcgcgatt ggcagggagc
    15661 cacggcggaa gtagatagat ttcggaagtt tgtgccacaa catgttttca gctcgcttag
    15721 caacggtttt cttgttgatc aggtgtttcg gaccaacgtt ttcagagatg gagttaccac
    15781 cccaggaacc acaacccaga gtcagggaag gtgcgagttt gaagttatac aggtcaccga
    15841 taccaccctg agaagccggg gtgttaatca ggatacgagc cgttttcatt ttctgaccga
    15901 agtaagaaac gcgagccggt tggttatcct ggtcagtgta caggcaagag gtatgaccga
    15961 taccgcccat agcaaccagt ttctctgctt tttctaccgc gtcttcgaaa tctttagcgc
    16021 ggtacattgc cagagtaggg gacagttttt catgtgcgaa cggttcgctt tcatcaacaa
    16081 cggtcacttc accgatcaga atcttggtgt tttctggtac agagaagcct gccagttcag
    16141 caattttata ggctggctga ccaacgatag ccgcgttcag cgcaccgttt ttcaggataa
    16201 cgtcctgaac agctttcagc tctttaccct gcaacagata gccgccgtgg gttgcgaaac
    16261 gttcacgtac tgcgtcataa acagagtcaa caacaacaac agactgttca gaagcacaga
    16321 ttacgccgtt gtcgaaggtt ttggacatca gcacagatgc aactgcacgt ttgatatcag
    16381 cagtttcatc gataacaaca ggcgtgttac ccgcgcctac accgatagct ggtttaccgg
    16441 agctgtatgc ggctttaacc atgcccggac caccagtcgc gaggatcagg ttgatgtctg
    16501 ggtggtgcat cagtgcgtta gaaagttcaa cagaaggttg atcgatccag ccgatcagat
    16561 ctttcggagc accggcagcg atagcagcct gcagaacgat atcagccgct ttgttggtgg
    16621 catcttttgc acgcgggtgc ggggagaaga taatggcgtt acgggtcttc aggctgatca
    16681 gcgatttgaa gatagcagtt gaagtcgggt tagtggtcgg aacgataccg caaataatac
    16741 cgattggttc agcgatagtg atggtaccaa aagtgtcgtc ttcagacaga acaccacagg
    16801 ttttttcatc tttataggcg ttgtagatat attcagaagc aaagtggttt ttgatcactt
    16861 tatcttcgac gatacccatg ccggattcgg caacggccat tttcgcgagt gggattcgag
    16921 catctgcagc agccagagcg gcggcgcgga agattttgtc tacttgctct tgagtgaaac
    16981 tggcatattc acgctgggct ttttttacac gctctacgag tgcgttaagt tcagcgacat
    17041 tagtaacagc cataatgctc tcctgataat gttaaacttt tttagtaaat catctgctcg
    17101 aatacgagag tatagtcagt gcggtgatga tttgcttaac cgatgaaaat caaaagctta
    17161 ctcgcgctca cactcactgt gatttactaa aagagtttaa acattagagt tattatctct
    17221 aatgcgtcac ttccaggtgg cgtaagcaag attactcact tctgggtact gattacgtga
    17281 tccaaatcaa atttttgcaa agctgacacc tttcagcatc gcttttcgcc attatagcta
    17341 acagttaata aattgtagta tgatttggtg gctacattag catgttttgc acaaccagat
    17401 aacaataacg aatgatagca attttaagta gttaggaggt gaaaaatgct gtcaaaaggc
    17461 gtattgtcag cgcgtctttt taaccttatt tatggctaac attatccggc ttttgcttcg
    17521 gagctaaccg tgattcagac cctttttgat tttcccgttt acttcaaatt tttcatcggg
    17581 ttatttgcgc tggtcaaccc ggtagggatt attcccgtct ttatcagcat gaccagttat
    17641 cagacagcgg cagcgcgaaa caaaactaac cttacagcca acctgtctgt ggccattatc
    17701 ttgtggatct cgctttttct cggcgacacg attctgcaac tttttggtat atcaattgat
    17761 tcgttccgta tcgccggggg catcctggtg gtgacgatag cgatgtcgat gatcagcggc
    17821 aagcttggcg aggataaaca gaacaagcaa gaaaaatcag aaaccgcggt acgtgaaagc
    17881 attggtgtgg tgccactggc gttgccgttg atggcggggc caggggcgat cagttctacc
    17941 atcgtctggg gtacgcgtta tcacagcatt agctatctgt ttggtttctt tgtggctatt
    18001 gcattgttcg ctttatgttg ctggggattg ttccgcatgg caccgtggct ggtacgggtt
    18061 ttacgccaaa ccggcatcaa cgtgatcacg cgtattatgg ggctattgct gatggcattg
    18121 gggattgaat ttatcgttac tggtattaag gggattttcc ccggcctgct taattaattc
    18181 ctttcaaatg aaacggagct gtcatgctcc gtttacttcg tcattatttt tactttgttc
    18241 ccgcgcagtt atcaaaagca aaaggaatgg gtgaaaatat tcttctcaaa ttacagttgg
    18301 ttataaggat ttccttaact gcttctcctc accatcatgt tattttcgcc acatcataat
    18361 cctgggcttg ctgaagaata attgaaatga tattagtaat tccactgctt ttggtagagg
    18421 aaagtgctaa ataataatca attgttaaat tattgtgcat ttcactactg gaactgtaat
    18481 cagaaaagag agacatgctt aaccaatctc tatttgattg aattaaaaga tgtttgttaa
    18541 gacatggatg caagctatag attctgatac ggtcaataaa agagaattgc ttaacaattt
    18601 tgcaaaatgt attggcgagt aagaagcgca tttggtactt tccgggcaac cgccagacga
    18661 ttctttattg gtaatgagaa taattaacaa ttaaagagcg tcgcgaaaga ataatgtgtc
    18721 tcgacagggg agacacagta cgaatcgaca taaggtgatc gtctgaatca ccagaataaa
    18781 taaagtcggt gatagtaata cgtaacgata aagtaacctg acagcagaaa gtctccgagc
    18841 ctgtgcaggg tcccaatccg ggattacaca tgctggttaa taccagtaat tataatgagg
    18901 gagtccaaaa aacaatgacc aacatcacca agagaagttt agtagcagct ggcgttctgg
    18961 ctgcgcttat ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc gtcacactgg
    19021 cggaaaaaca aacgctggta cgtaacaatg gttcagaagt tcagtcatta gatccgcaca
    19081 aaattgaagg tgttccggag tctaatatca gccgagacct gtttgaaggc ttactggtca
    19141 gcgatcttga cggccatccg gcacctggcg tcgctgaatc ctgggataat aaagatgcga
    19201 aagtctggac cttccatttg cgtaaagatg cgaaatggtc tgatggcacg ccagtcacag
    19261 cccaagactt tgtgtatagc tggcaacgtt ctgttgatcc gaacaccgct tctccgtatg
    19321 ccagttatct gcaatatggg catatcgccg gtattgatga aattcttgaa gggaaaaaac
    19381 cgattaccga tctcggcgtg aaagctattg atgatcacac attagaagtc accttaagtg
    19441 aacccgttcc gtacttctat aaattacttg ttcacccgtc aacttcaccg gtgccaaaag
    19501 ccgctatcga gaaattcggc gaaaaatgga cccagcctgg taatatcgtc accaacggtg
    19561 cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc agcccgacct
    19621 actggaacaa cgcgaaaacc gttattaacc aggtaaccta tttgcctatt gcttctgaag
    19681 ttaccgatgt caatcgctac cgtagtggtg aaatcgacat gacttataac aacatgccga
    19741 tcgaattgtt ccagaagctg aaaaaagaga tcccggacga agttcacgtt gatccatacc
    19801 tgtgcactta ttattacgaa attaacaacc agaaaccgcc atttaacgat gtacgtgtgc
    19861 gtaccgcact gaaactgggt atggaccgcg atatcattgt taataaagtg aaagcgcagg
    19921 gcgacatgcc cgcctatggt tacactccac cgtatactga tggcgcaaaa ttgactcagc
    19981 cggaatggtt tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa ctgctggctg
    20041 aagcgggtta taccgcagac aaaccgttaa ccatcaacct gttgtataac acctccgatc
    20101 tgcataaaaa gctggcgatt gcagcctctt cattgtggaa gaaaaacatt ggtgtaaacg
    20161 tcaaactggt taaccaggag tggaaaacgt tcctcgacac ccgtcaccag ggcacttttg
    20221 atgtggcccg tgcaggctgg tgtgcagact acaacgaacc aacttccttc ctgaacacca
    20281 tgctgtcgaa cagctcgatg aataccgcgc attataagag cccggccttt gacagcatta
    20341 tggcggaaac gctgaaagcg actgacgagg cgcagcgcac agctctgtac actaaagcag
    20401 aacaacagct ggataaggat tcggccattg ttcctgttta ttactacgtg aatgcgcgtc
    20461 tggtgaaacc gtgggttggt ggctataccg gcaaagatcc gctggataat acctacaccc
    20521 ggaatatgta cattgtgaag cactaatggc aatacgtggg gcaggcgtgt cctgctccac
    20581 ggtgtctgat ttttatcgca ttacagaagg cacaggccag aaggtagggc aatgttaaaa
    20641 tttattctac gtcgctgtct ggaagcgatt ccgacgctat ttattcttat tactatttcg
    20701 ttcttcatga tgcgcctcgc gccgggaagc ccttttaccg gcgaacgtac tttaccgcca
    20761 gaagtgatgg ccaatatcga agcgaaatat catcttaatg atccgatcat gacacagtat
    20821 ttcagctacc tgaaacaact ggcgcatggc gatttcggtc catcgtttaa atataaagat
    20881 tattcggtca acgacctggt tgcatccagt tttcccgttt ctgccaaact gggagccgca
    20941 gcatttttcc ttgcggtaat actgggtgtt agtgctggcg ttattgcagc attaaaacaa
    21001 aacaccaaat gggactatac cgtgatgggg ctggcaatga ccggggttgt tatccccagt
    21061 tttgtggttg cgccattatt agtcatgata tttgcgatca ttttgcattg gctgccgggc
    21121 ggtggctgga atggtggtgc gcttaaattc atgatattgc cgatggtggc gttgtcactc
    21181 gcctatatcg ccagtattgc gcgtattacc cgtggctcta tgattgaggt tttacactcc
    21241 aactttattc gtactgcccg ggcgaaaggg ttacctatgc ggcggatcat tttacgccac
    21301 gcattaaaac ctgctctgtt acccgtgctc tcctatatgg gccccgcatt tgtcggcatt
    21361 attaccggtt ctatggttat cgaaaccatt tatggtttgc cggggattgg gcaactgttc
    21421 gttaatggcg cattgaaccg tgactattcc ttagtgttaa gcctgactat cctggttggt
    21481 gcgttaacca ttttgtttaa tgccattgtc gatgtgctat atgcggttat cgacccgaaa
    21541 atccgttact gatactggag ctcgcgatga tgttaagtaa gaaaaacagc gagacgctgg
    21601 aaaatttcag tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac gcacgtcgac
    21661 gttttatgca taaccgtgcg gcggttgcca gtctgatagt gctggtgctg atcgcgttat
    21721 ttgtcatcct ggcaccgatg ctttcgcagt ttgcctatga cgatactgac tgggcgatga
    21781 tgtccagcgc cccggatatg gagtccggtc actactttgg tactgactca tccggtcgcg
    21841 acctgcttgt gcgcgttgcg attggcgggc gtatctcact catggtcggt gttgctgcgg
    21901 cactggtggc tgtggtcgtc gggacacttt atggttcgct ttccggttat ctgggcggta
    21961 aagtggattc ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca ttcatgttct
    22021 tcgtcatttt gctggtgacc tttttcggtc aaaacatcct gctgattttc gtggcgatag
    22081 gcatggtttc ctggctggat atggctcgta ttgtgcgtgg gcaaaccctg agtctgaagc
    22141 gcaaagagtt tattgaggcg gcacaagttg gcggtgtatc gacgtcgggc attgttattc
    22201 gccacattgt gccgaacgta ctcggtgtgg tggtggtcta cgcatcactt ctggtgccca
    22261 gcatgatcct ctttgaatct ttccttagct tcctggggtt gggtacgcaa gagccgttaa
    22321 gcagctgggg tgcattgctg agtgatggcg cgaactcgat ggaagtctct ccatggttat
    22381 tgttgttccc agcgggattc ctcgtggtga cactgttttg tttcaacttt atcggcgatg
    22441 gcttgcgtga tgccctcgac ccgaaagatc gttaaggagt gcagccatga gcgtaattga
    22501 aactgcaact gtgccgctcg cacaacaaca ggctgacgca ctgctgaacg tgaaagattt
    22561 gcgtgtcacc tttagtaccc cggacggcga cgtcacggcg gtcaatgatt tgaatttttc
    22621 cctacgtgcc ggagaaacgc tgggcattgt aggtgagtct ggttcgggta aatcgcaaac
    22681 tgcatttgcg ttgatgggcc tgttggctgc caacggacgt attggcggat cggcaacctt
    22741 caatgggcgt gaaatcctca atttgccaga gcatgaactc aataaactgc gcgctgaaca
    22801 aatctcaatg atttttcagg acccaatgac ttcgttgaat ccctatatgc gtgtcggtga
    22861 gcagttgatg gaagtgctga tgctgcataa gaacatgagc aaagctgagg cgtttgaaga
    22921 gtcggtgcgg atgctcgatg cggtaaaaat gccggaagca cgtaaacgca tgaaaatgta
    22981 cccgcatgaa ttttctggtg gcatgcgtca gcgagtcatg attgcgatgg cattgctatg
    23041 tcgacctaag ctgctgattg cggatgaacc cactactgcg ctggacgtca ccgtacaggc
    23101 gcagatcatg acgctattaa atgaactgaa gcgggaattt aataccgcca tcattatgat
    23161 cacccacgat cttggcgtgg tggcggggat ctgcgacaaa gtgctggtaa tgtacgctgg
    23221 gcgcacgatg gaatatggca atgcgcgcga tgtcttttat caacccgttc atccttattc
    23281 tatcggtttg ctcaacgcgg tgccgcgtct cgatgcggaa ggtgaaacaa tgttgaccat
    23341 cccgggtaat ccgccaaact tgctgcgatt accgaaaggt tgcccgttcc agccacgttg
    23401 tccacatgcg atggaaattt gtagtagcgc accgccgctg gaagagttta cgcctggccg
    23461 tctgcgtgct tgctttaaac cggtggagga actgttatga atgctgtaac tgaaggaaga
    23521 aaagtcctcc ttgaaatcgc cgatctgaaa gtgcactttg aaatcaaaga tggcaaacag
    23581 tggttctggc aaccgccgaa aacgctcaaa gccgtcgatg gtgtcactct tcgcctgtat
    23641 gaaggggaaa cattaggtgt ggtaggggag tcgggatgcg gtaagtccac ctttgctcgc
    23701 gccatcatcg gtttggtcaa ggcgaccgac ggtcatgttg cctggttagg taaagagttg
    23761 ctgggcatga agcccgatga atggcgtgcc gttcgcagtg atattcagat gattttccag
    23821 gatccgttgg catcgctaaa cccgcgtatg accatcggcg agattatcgc tgaaccgctg
    23881 cgtacctatc atccgaaaat gtcacgccag gaagttcgcg agcgcgtgaa ggcgatgatg
    23941 ctgaaagtcg ggttattgcc taacctgatt aaccgctatc cgcatgagtt ctctggtggg
    24001 cagtgccagc gtatcgggat tgctcgtgct cttattcttg aaccgaagct gattatctgt
    24061 gatgagccgg tgtcggcgct ggacgtgtca attcaggcgc aggtggtcaa cctgctccag
    24121 cagctgcaac gcgagatggg attgtcatta atttttatcg ctcacgacct ggccgtggta
    24181 aaacacattt ccgatcgtgt gctggtgatg tatctcggcc atgcggtaga actggggacc
    24241 tatgatgagg tctaccacaa tccactacat ccttacacca gggcattgat gtcggcagtc
    24301 cccatacctg atccggatct ggagaagaat aaaaccatcc agttactgga aggggaatta
    24361 ccgtcgccga tcaacccgcc ttccggttgt gttttccgta cccgttgccc gattgccggt
    24421 ccggagtgcg ccaaaacacg tcctgttctg gaggggagtt tcagacacgc cgtttcttgc
    24481 ctgaaagtcg atccgcttta aacacaataa gggctgacag ttgtcagccc tttttcacgc
    24541 cgaaagtgat tatttattcc cgccagatga tatggcaaag tttgtgatct ttttcgcggc
    24601 ataacagaat gcgggcgaaa acatcgttga tttcaccatc ttcactgtcc gccagaccaa
    24661 tcaccacttc ggcaaaaaag tccgggttca aatcgaaatc aacatgttcc tgccagtctt
    24721 ccgccggatc gaataactcc gcgccgccgc gctcttcaaa ctgaagattg aacagcagaa
    24781 catcagctgg atcgagattg tcggcagcca gttcgagaaa aatatcgtag gcctgctcaa
    24841 gcgtttcatc ttcagtcagg cgattgttta gatccatatc catagtcact acctgtttaa
    24901 cctctgttgg cgacgtttta cagcaacggg ctgaagaagt aaaacagtcg ctcggcgaca
    24961 cgttgccata atggacgttt tagccataaa cgggcatcga gcagacgaga acgcgaaata
    25021 taatcgtcct gaacggcggc gaggtcagca ccaaaccctt tatcgtcgat tgccagggta
    25081 atctcgaaat tcagccacag actacgcata tcaaggttaa cggtaccaac cagacttaat
    25141 tcgccatcga ccagcacgct cttggtatgc aataacccgc cttcaaactg ataaatttta
    25201 accccagcag ccagcagttc cgtaaagaac gcgcgactgg cccagccaac cagcattgaa
    25261 tcattttttc gcggaaggat aatactgaca tccaccccgc gctgcgccgc cgtgcaaatc
    25321 gcatgaagta aatcatcgct gggcacaaag tagggcgtcg tcatgatcaa atattcacgt
    25381 gccgaataag ccgcagtcag taatgcctgg tgaatgagat cttccggaaa gccggggcca
    25441 gaagcaattg tgtgaatggt gtgaccgctg gcctgctcaa acggcataat attgacatct
    25501 ggtggtggcg gcagaatacg tttcccggtt tcaatttccc agtcgcagga ataaataatc
    25561 cccatcgcgg tagcgatggg accttccata cgcgccatca gatcaatcca ttgccctacg
    25621 cccgcatctt gtttgaagta gcgagggtcg accatattca tgctgccggt gtacgcgatg
    25681 taattatcga tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg taaaaacaca
    25741 cgcatcagat tgacctttaa ggcttcgacc acttcaatac cggcattacg cattagctcg
    25801 ggccacgggc tgcggaaaaa agccacactc ccggcggagt cgagcatcaa tcggcagtga
    25861 atgccgcgtc gcgcagcggc cattaatgat tcagccacct ggtccgccat gccgccgggc
    25921 tgccagatat aaaacaccat ctcaatatta tggcgcgcga gctggatgtc gcggattaac
    25981 gcctgcatca catcatctga ctcggtcatc agttgtagct gattcccttt gaccccagcg
    26041 atcccctgac gacgctcaca aagcttgaat agtggcgcag cgacactgct attttcttcg
    26101 gcgaagatat gcttacaggc tttaaggtcg ttaagccact ttgcggtgga aggccacatc
    26161 gctctggcgc gctcagcgcg gcgtttgcct aaatggagct cgccaacagc aagataggca
    26221 ataattccga ctaacggcag aatgtaaata atcaacagcc aggccatcgc ggagggaact
    26281 gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca gtatcccaga
    26341 atggccaacc aactcaccaa cgtataaacg gttgtcatag attagaaatc cttttgaaag
    26401 cgcattgtta tgagtttacg catatggatt catctggcaa ataaaaacgc gggaaaagca
    26461 ctggtcagcg tcgggggcgg gggtataatg accattctgt tattgcatag agtagttaac
    26521 atgaagcgga gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc tagccgacgt
    26581 aaatcgcgtt ggcttgaggg gcagtcgcgc cgaaatatgc gtatccacag catcaggaag
    26641 tgcattctaa acaaacagcg gaactcgtta ttgtttgcga tctacaatat ctaaatgtac
    26701 tgggcaccat tgcggtgccc aataagttta ctcttccgtc tttaaactct atttttgcgc
    26761 cgattcttta ttgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa taccatgacc
    26821 ggagttcaac aacattgaaa cgagcatatc attattaatc tcttcaccat ttaaaactcg
    26881 cgccagaatt tcgctgccaa acagttgcgg tgagagaatg atatccggat gtaccatctt
    26941 aatcttattc aggtttttgc tatcactgac ggcgagaact gttttgacat cactgctcat
    27001 atcttttgcc gagagtacaa caaacgcgtt atctgcatcg ttatcactca gcgccagaat
    27061 ggcccggcat cgatcgattc ccgctttctt taatactgaa ctgtcattac tgtcaccggg
    27121 gataacatca gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat cttcaggcaa
    27181 gttgctgata accgttacgt tttgtccgcg ttgattcagt tgcaggatcg tattgatggc
    27241 gagaatcgaa tgtccgcaaa caataaaatg atctttacga tgcattgtat gattgtttcc
    27301 ttttacaagt ttgttgaatc ccccgcggat aagcggacca aaaattgagg tcatagaggt
    27361 ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg ctgattcaga
    27421 aacagggaca atatcgccat agccgacggt tgacatggtt tctatcgaaa aatagaacgc
    27481 ggtcatcaaa ctttctattc gcggattaaa accttcgctt aaataaagcg caccgtaggt
    27541 ggagtaaaac agtaacgtcg tgaaactaat aaatgcgaaa attgtcccgg ctgcggcgct
    27601 actgtgggag aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa tgcaaaatcc
    27661 aatactaaat ttcagccagg gataaaaatg cagggtgtaa attagcgcta tcaacaacaa
    27721 aatgatactg attgcccatg cgagctttgc ccggaacagt aagccaatgg agttcaggac
    27781 aagaaagacg ccgagcataa agagtggggc gtttgccagc aaggagagat ccaactctga
    27841 aaatgctttg atatggaaaa tatctagcaa attgaccgac ataccataga ttgttttaaa
    27901 aataagcaat ccatttaaaa agacggccag cgcgagaatg tcgtgccgta gggtcaccca
    27961 taaattagtt gctgtttgtt tgaatgtagc ccagtgactc actgatacct ctgtaaccac
    28021 aattttgttt aattatctga gcgtgcacat tttctgaaca tacatgcagc gcgtgaatgt
    28081 gttaaacaat agtagatgac tttatccgca gtaatcaaag gttttgagtg agcaactctg
    28141 cgtcaggaaa gggcattttt cctgaatatg cttacatcat ataaagaaat acagataaag
    28201 ttattatctg cttgtggggg tgaatgcact gaccgaccta taaggaaagg ccaaacaaga
    28261 acacggttgc aaaaaccgtg cccttaaata ttgaatctct attcagaaca ctttcttaaa
    28321 tggtttcact gaaacgtgtt catagactcc tgccgctacg tacgggtcag catcggccca
    28381 ggcctgagct gcttccagcg attcaaattc agcaataacg gttgagccag taaatcccgc
    28441 agcccctgga tcgttactgt ctaccgctgg cattggacca gctgtcaaca aacgaccttc
    28501 atcatgcagt aactgtaaac gtgctaaatg tgccggacga acggaaaggc gtttttcgag
    28561 gctatcagct ttgtcttgag cgtaaataac atacaacacg ggcacaactc cttgttcggg
    28621 aaagttgtaa agtacgttat ttgaaagggc gaagatctgc aacggaaaga tgatgtcttt
    28681 gttaagacca tgcataaagt aaggtcaatt acgccaaaaa tgacattttc actgatcctg
    28741 atcgtcttgc cttattgaat atgattgcta tttgcattta aaatcgaggc ctggtttttc
    28801 tactgaaatg attatgactt caatgaccct tgatttacct cgccgcttcc cctggccgac
    28861 gttactttcg gtctgcattc atggtgctgt tgtggcgggt ctgctctata cctcggtaca
    28921 tcaggttatt gaactacctg cgcctgcgca gccaatttct gtcacgatgg ttgcgcctgc
    28981 ggatctcgaa ccgccacaag ccgttcagcc gccaccggag ccggtggtag agccagaacc
    29041 ggaacctgag ccgatccccg aaccgccaaa agaagcaccg gtggtcattg aaaagccgaa
    29101 gccgaaacct aagccaaaac cgaagccggt gaaaaaggta caggaacagc caaaacgcga
    29161 tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa aatacggcac cggcacgccc
    29221 gacatcaagt acggcgacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc
    29281 acgcgcatta agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga
    29341 agggcaggtt aaagttaaat ttgacgtcac gccggatggt cgcgtggata acgtacaaat
    29401 cctctcagcc aagcctgcga atatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg
    29461 gcgttatgag ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa
    29521 cggcaccacc gaaattcagt aagcataaag tcaaaagcct ccgaccggag gcttttgatt
    29581 attactcaac aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata
    29641 atgcttctgt cgctttatag cgttgcccga tgggttcaga cgcgactttt ttcacccaca
    29701 cttcaatatt aatgctaacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca
    29761 ccacatcgcc gaccgcaacc ggtcgtaaga aagtcattcc ttcaacccgc acggtcacta
    29821 cgcgaccgtg ggcaatttct ttcgccagaa tagcgccgcc aatatccatt tgtgacatta
    29881 accaaccacc aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta
    29941 aaacaagatc gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg
    30001 aaaatcacag gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc
    30061 agccccgaag gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat
    30121 atagataccg cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa
    30181 gttgacccaa atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag
    30241 gataaagaaa acagcccagg ccagattcag cttcgaccat actggttgcg gcagcgtgag
    30301 ttctttaccc agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag
    30361 ggctcccgca aacagagcat aaatgactgt aaccttccat ttaataaact catcattgtg
    30421 gaagaacagc gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt
    30481 ctcaacctta cgaaagcgaa cccagctata tataagcaca atcgccgtgg cgacgatcag
    30541 cgccgcagta gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg
    30601 taaaaaatca agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg
    30661 cgaatcaaca tatacaggcg gaacagatag atgagcagta cggctgaaat caggttgctc
    30721 aaggtgttcg ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa
    30781 agcagcaaca gtgtctttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata
    30841 ttcgcccaag tcagccgcat actgctacgc atcgaggcaa aaacgcccat tttgtcctgt
    30901 accaacatca ccggtgccag agccagtaaa atggccatga taattcccgg aacgaccacc
    30961 agcatgatgc caatctgtac taaaagggta gtcagaaaaa tcagaataaa taactttggc
    31021 aatatcggcg cactggcacc aatagctcga agcgcactga ctctctgacc cgcagacacc
    31081 agctggataa ttaatattac gcctccggcg agaatggcgt taccgattaa tcctgaaaaa
    31141 gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc
    31201 aggtcgaaca acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt
    31261 gcatcactgg gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat
    31321 accaacagaa tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac
    31381 tgcgccgtga tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat
    31441 tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg tatggaaaag catatctttg
    31501 taaagcggag gtaattgctg gccttcaggc gtgggcaaac cgctgcaagt ggcgagcgag
    31561 ttaatatttg cgtagcgaaa atatttaaaa attgatttaa atcacattaa ccaggatttt
    31621 caatgcaact tctaaattaa tccagatcaa taaatggtga attatcatat gtaatgtgac
    31681 ctatgtagga tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa
    31741 cgacggagcg gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctctc
    31801 tggcagtgcc tatgcgcatg aagcaggcga attttttatg cgtgcagggt ctgcaaccgt
    31861 acgtccaaca gaaggtgctg gtggtacgtt aggaagtctg ggtggattca gcgtgaccaa
    31921 taacacgcaa ctgggcctga cgtttactta tatggcgacc gataacattg gtgtggaatt
    31981 gctggcagcg acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac
    32041 cgttcatcat ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa
    32101 attccgtcct tacgttgggg caggtattaa ctacaccacc ttctttgata atggatttaa
    32161 cgatcatggc aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc
    32221 cgggcaggtg ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtctg
    32281 gtacatggat atcgatacca ccgccaatta taagctgggc ggtgcacagc aacacgatag
    32341 cgtacgcctc gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccgcac
    32401 aaaaacgacc ccgtaatata cggggtcaat aaggacatgg tataaagggt attatttctt
    32461 cgcttctacg ccatcagttt cagagcgaat taaaaatttc tcagttgttt gcggaatatg
    32521 ctgaatcagc cagtcggcca tttgcttttc ctcatttaaa atcgcttcga tggttggaat
    32581 tgaagctgta tcaccggcat tttttgctgc tgctaatagt gaggtgtaac aggcgatttc
    32641 aaattgctcg aagacatatc cgctaataga gcctttgact atttcatcag aagggaatat
    32701 accaccgatt gactgcccaa gcgcagccat tttactcatg gaatctttaa tgactgaacg
    32761 tgaaatgtca ttacgatcaa gaatagtttc cagttgaata atctggtttt tggtttcact
    32821 aagatgttgt tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga
    32881 ttcaagcata gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata
    32941 atgttcaata cgattcatga aaatgtcctc tttataaaat gactaaaagt taaaattcat
    33001 atttcaggct ttattttcgg ctttcttatt tacgttatta agggccagat cagtcaattt
    33061 gatgtcggtg gccttttctt cttccagggt ctctttcaga agcttcgctg ctttacgata
    33121 acctaattgt tcagccagcg tcgctaatgt cccgtaactg gcgatctcat aatgctcgac
    33181 tttctgtgct gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat
    33241 gacctcatta gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt
    33301 caggttcgat tctgattcca caacttgatc aatacgttca atctgtccat gtgtttcctc
    33361 gaggtgcgca tgaaaagcct gacttaattt ttcgtttgat gttgctcttg cgagttttgc
    33421 cagtgcccgg gttaattgtt tttctgcgct gtaggtatct gaaagcaggt gaataaatac
    33481 atcttcaatg gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc
    33541 ccgcagatgc aattatcttt atcagctgat attaataatt aacggtgaat caggatttgc
    33601 ctgatttatt accaccgctt tgttgaccgc cttttttacc cgcttcagat gcgcgttgcg
    33661 gatcattttt aaaattaccg ccgctatgct gaccgccttt acggccagcg tcggatgcct
    33721 tttcacggtc ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc
    33781 cgttgcgttg cattgtttca ttaatatgag tgttgtgtgt cgacactcat taaaattagt
    33841 tgctaatgaa aattagtcaa attaagcgca acgagaagaa agagggaaaa tatattatga
    33901 ggaacgttct tgatatatta acaattgcct gaggaataag tgacttagag aaaattattg
    33961 attttactgg tgttatgttg cgggattaat ttatttgatt atattactgt tgggcggaaa
    34021 aatgac
//
LOCUS       DAYPHN010000034        33427 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000034 DAYPHN010000000
VERSION     DAYPHN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.034"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(144..1535)
                     /gene="narK"
                     /locus_tag="ACG0BW_001605"
     CDS             complement(144..1535)
                     /gene="narK"
                     /locus_tag="ACG0BW_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFK7258580.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            1874..3670
                     /gene="narX"
                     /locus_tag="ACG0BW_001606"
     CDS             1874..3670
                     /gene="narX"
                     /locus_tag="ACG0BW_001606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFK7258581.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPTLMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            3663..4313
                     /gene="narL"
                     /locus_tag="ACG0BW_001607"
     CDS             3663..4313
                     /gene="narL"
                     /locus_tag="ACG0BW_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFK7258582.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDISVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(4314..5708)
                     /gene="ychO"
                     /locus_tag="ACG0BW_001608"
                     /gene_synonym="iatA"
     CDS             complement(4314..5708)
                     /gene="ychO"
                     /locus_tag="ACG0BW_001608"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFK7258583.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGLAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            5894..6247
                     /gene="ychN"
                     /locus_tag="ACG0BW_001609"
     CDS             5894..6247
                     /gene="ychN"
                     /locus_tag="ACG0BW_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFK7258584.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(6291..6986)
                     /gene="chaC"
                     /locus_tag="ACG0BW_001610"
     CDS             complement(6291..6986)
                     /gene="chaC"
                     /locus_tag="ACG0BW_001610"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFK7258585.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(7144..7374)
                     /gene="chaB"
                     /locus_tag="ACG0BW_001611"
     CDS             complement(7144..7374)
                     /gene="chaB"
                     /locus_tag="ACG0BW_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFK7258586.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            7644..8744
                     /gene="chaA"
                     /locus_tag="ACG0BW_001612"
     CDS             7644..8744
                     /gene="chaA"
                     /locus_tag="ACG0BW_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFK7258587.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIGINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            9150..9257
                     /locus_tag="ACG0BW_001613"
     CDS             9150..9257
                     /locus_tag="ACG0BW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFK7258588.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            9685..9792
                     /locus_tag="ACG0BW_001614"
     CDS             9685..9792
                     /locus_tag="ACG0BW_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFK7258589.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(9940..10794)
                     /gene="kdsA"
                     /locus_tag="ACG0BW_001615"
     CDS             complement(9940..10794)
                     /gene="kdsA"
                     /locus_tag="ACG0BW_001615"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFK7258590.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(10830..11639)
                     /gene="sirB1"
                     /locus_tag="ACG0BW_001616"
     CDS             complement(10830..11639)
                     /gene="sirB1"
                     /locus_tag="ACG0BW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFK7258591.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(11643..12035)
                     /gene="sirB2"
                     /locus_tag="ACG0BW_001617"
     CDS             complement(11643..12035)
                     /gene="sirB2"
                     /locus_tag="ACG0BW_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFK7258592.1"
                     /translation="MTIFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(12032..12865)
                     /gene="prmC"
                     /locus_tag="ACG0BW_001618"
     CDS             complement(12032..12865)
                     /gene="prmC"
                     /locus_tag="ACG0BW_001618"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFK7258593.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(12865..13947)
                     /gene="prfA"
                     /locus_tag="ACG0BW_001619"
     CDS             complement(12865..13947)
                     /gene="prfA"
                     /locus_tag="ACG0BW_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFK7258594.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(13988..15244)
                     /gene="hemA"
                     /locus_tag="ACG0BW_001620"
     CDS             complement(13988..15244)
                     /gene="hemA"
                     /locus_tag="ACG0BW_001620"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFK7258595.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            15458..16081
                     /gene="lolB"
                     /locus_tag="ACG0BW_001621"
     CDS             15458..16081
                     /gene="lolB"
                     /locus_tag="ACG0BW_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFK7258596.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            16081..16932
                     /gene="ispE"
                     /locus_tag="ACG0BW_001622"
     CDS             16081..16932
                     /gene="ispE"
                     /locus_tag="ACG0BW_001622"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFK7258597.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LCDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            17083..18030
                     /gene="prs"
                     /locus_tag="ACG0BW_001623"
     CDS             17083..18030
                     /gene="prs"
                     /locus_tag="ACG0BW_001623"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFK7258598.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            18155..19834
                     /gene="dauA"
                     /locus_tag="ACG0BW_001624"
     CDS             18155..19834
                     /gene="dauA"
                     /locus_tag="ACG0BW_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFK7258599.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(19889..20167)
                     /gene="ychH"
                     /locus_tag="ACG0BW_001625"
     CDS             complement(19889..20167)
                     /gene="ychH"
                     /locus_tag="ACG0BW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFK7258600.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            20445..21029
                     /gene="pth"
                     /locus_tag="ACG0BW_001626"
     CDS             20445..21029
                     /gene="pth"
                     /locus_tag="ACG0BW_001626"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFK7258601.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPLGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            21146..22237
                     /gene="ychF"
                     /locus_tag="ACG0BW_001627"
     CDS             21146..22237
                     /gene="ychF"
                     /locus_tag="ACG0BW_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFK7258602.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            23082..25949
                     /gene="ycgV"
                     /locus_tag="ACG0BW_001628"
     CDS             23082..25949
                     /gene="ycgV"
                     /locus_tag="ACG0BW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFK7258603.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKYGAIGLNAIIFSPLL
                     IAADLGSQNGTNITISDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAVANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSSGSYGASAQTATAVINMQNTDITVDRNDSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVTMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HTSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTIPEPT
                     PNPEPTPAPAQPPIVNPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDEMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAHSGFYSDLVVKASRQKNSFHVLDSQNNG
                     VNANGTANGMSIALEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(26049..27968)
                     /gene="dhaR"
                     /locus_tag="ACG0BW_001629"
     CDS             complement(26049..27968)
                     /gene="dhaR"
                     /locus_tag="ACG0BW_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFK7258604.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            28196..29266
                     /gene="dhaK"
                     /locus_tag="ACG0BW_001630"
     CDS             28196..29266
                     /gene="dhaK"
                     /locus_tag="ACG0BW_001630"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFK7258605.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAEHGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            29277..29909
                     /gene="dhaL"
                     /locus_tag="ACG0BW_001631"
     CDS             29277..29909
                     /gene="dhaL"
                     /locus_tag="ACG0BW_001631"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFK7258606.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITIQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            29920..31338
                     /gene="dhaM"
                     /locus_tag="ACG0BW_001632"
     CDS             29920..31338
                     /gene="dhaM"
                     /locus_tag="ACG0BW_001632"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFK7258607.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            31657..33354
                     /gene="treA"
                     /locus_tag="ACG0BW_001633"
     CDS             31657..33354
                     /gene="treA"
                     /locus_tag="ACG0BW_001633"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFK7258608.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQNEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQKEAEPTP"
BASE COUNT         8433 a         8565 c         8304 g         8125 t
ORIGIN      
        1 agtattcccc atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccact
       61 cacacataag tatgttgtaa ctaaacgaca gataaaaaaa agcgcgacat catgccgcgc
      121 aaaggataat caaatattaa gatttatttt ttagaatgcc gaccatatac cgcccaggta
      181 atcaccacgc aggcgatata gaaaatcaaa aataccttca ttgcgccgac tggcgaaccc
      241 gttaatgcca gcgagctacc aaacgctttc gggataaaga agccaccaat agcgccaatc
      301 gcagagatga aacccagcgc cgccgccgtg tcggttgccg cttcacgcat cgctcgttcg
      361 tcagaacccc cttctgcttt cacgcgatcc attgtcagtt tacggaagat cacggaaatc
      421 atctggaaag tggaaccact acccagccca gctgtcagga acagcgccag gaagactgcg
      481 aagaacgcca tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg
      541 ctgaaaatcg ccatcaggat aaagttcacc agtgtgacac gagttccgcc cagacggtca
      601 gataatgcac cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat
      661 tgcagaatct gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc cgcggagaag
      721 ccgatgaagg agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt
      781 ttgagtaccg gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca
      841 aaccacgccg caatggtgaa gatggcaagg aacggcaccc atacccagga cgcattcgcc
      901 agatacagct cagtcccatc cggctgtttg acaccctggc taccaaatac tgcgaaaatc
      961 gacagtgata ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gttgcccaga
     1021 ccaccattca ggcccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt
     1081 gccatactcg atgcgaagtt cgcgccagca aagccgcaca gcagagagat gatgatgaag
     1141 acgctataag gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc
     1201 agaataccag tgctgaacgc cgtccagcga cgaccaccga agataggaac cataaaggag
     1261 tatggaacac gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga
     1321 tcggtcgtaa aattaaaacc gactttcggt aagttcaccg caacagcgct gaacaacatc
     1381 catacgcaaa acgccagcag cagacaggga acggaaatcc acaggttgcg gctggcaata
     1441 cgttgaccgc gttgttgcca gaacgcagga tcttccggtc gccaatctgt aatgacagct
     1501 ccagtagccc tttcgggggc ggatgagtga ctcatagaca cctctgatac tcgtttcgat
     1561 gtctgccacc ttagtgtcta tagctaaagg caatttgatg taaatcaaac gataagataa
     1621 ctttatctat cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt
     1681 atgagatttt tcacggtgct gtaaaatccc tacccttacc gatgtaaagc gactaaccac
     1741 gcggcaaata aggagtaact ctttccgggt atgggtatac ttcatccaat agccgagaat
     1801 actgccattc cagaatgtat cgtcacattc attaaggtta ttgctcattt aaagcctgaa
     1861 ggaagaggtt tacatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc
     1921 gcttattgtg ttgctttcta ctgctattgg actggcaggg atggcagttt ctggctggct
     1981 agtgcaaggc gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca
     2041 aagttaccgt ctgttggcag cagtgccatt aagcgagaaa gacaagccct taattaaaga
     2101 gatggaacaa acagcattta gcgccgaatt aacccgagca gcagaacgag acggacaact
     2161 ggcgcaatta cagggtttac aagattactg gcgtaatgaa ctgatcccta cgctgatgcg
     2221 tgcacaaaac cgcgaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca
     2281 gctggtatct ggttttgacc gcaccacgga aatgcgcatt gagacagtgg tactggtcca
     2341 tcgggtaatg gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc
     2401 gcgattgcta caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gccatcgcga
     2461 ttttacccaa cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcatt
     2521 gaacaatatg tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga
     2581 gaaaactgcc gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg
     2641 ccgtttgcat tcccgcgccc cgctgtgtga acgcctgtca cctgtactca acggcttaca
     2701 gaatttaacc ctgctacgtg atatcgaact gcgggtgtat gacactgatg atgaagagaa
     2761 tcatcaggag tttacctgcc agccagatat gacttgtgat gataaaggct gccagctctg
     2821 cccgcgcggc gtattacccg ttggcgatcg cggcacaacc ctgaagtggc ggctggctga
     2881 ctctcatacg cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca
     2941 tgatcaacaa caactggtgg ataccctggt tgaacaactc accgccacgc tggcgttgga
     3001 tcgccatcag gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg
     3061 cgaactgcat gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt
     3121 acagatgcag ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa
     3181 cgaactgaat gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct
     3241 caccgagcct ggattacgtc cggcgctgga agcgagttgc gaagagtaca gcgccaaatt
     3301 tggcttcccg gtgaagctgg attatcaatt gccgcctcgt ctggtgccct cgcatcaggc
     3361 aatccacttg ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc
     3421 gagtgaggtc gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga
     3481 taacggctgc ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat
     3541 gcgcgaccgt gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg
     3601 caccgaagtg gtggtcacct ttattcccga aaaaactttc actgacgtcc aaggagatac
     3661 ccatgagtaa tcaggaaccg gctactatcc tgctgatcga cgatcacccg atgctgcgaa
     3721 ctggcgtaaa acagcttatc agtatggcac cagatatcag cgtggttggc gaagcgagta
     3781 atggcgaaca gggtattgaa ctggcggaat ctcttgatcc cgatctgatc ctgttagatc
     3841 tcaatatgcc cggcatgaat ggtctggaaa cgctggataa actccgcgaa aagtccctct
     3901 cagggcgcat tgtggtattc agcgtctcta accatgaaga agatgtggtc accgcactga
     3961 aacgcggcgc ggatggctat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat
     4021 tgcatcaggc agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg
     4081 ccagcttgcg cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg
     4141 agcgcgatat tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc
     4201 tggacatcac cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc
     4261 tcaagtctcg cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt
     4321 tcccagcgca gttcgtcgtt tgacaatgcg tcgaacggtt cgacaagcgt tagcgtgatc
     4381 tcattggagg agacacgctg cccctggtta tcttccacca ccaccgacaa tcgccagtga
     4441 ttgctcgccc tttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg
     4501 ttggcttgtg cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc
     4561 cgaataccgt aacgactgcg gatttgtaat ttcagcggca ctgtttcgcc aggttttaga
     4621 tcccacggcg gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc
     4681 ggtagattat ttcgctgcgg attatcatag cgactaccac gtaacgactg actttcggca
     4741 acctcgcccg cagaaagttg ttttttgagc gggacaccaa agcggtagtt aagattcagc
     4801 ccgaggttat tttgattctc accactttca ccctgtttat gctgggccgt cacagtgact
     4861 aagggcacag gggtgtaatt taatcccaga ctcaatgcga cgggattgtg ataacccgta
     4921 ccagagttaa acaaatcaac acgatcacca aaatactgtt ctacgctgac actggtattg
     4981 aggtgttgat aaaacggcat gcgcatccgg gctgtcaggt cgtacccgcg cgccatccgt
     5041 tgttcctgcg tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat
     5101 agtcgcaaat attcgcccca cgcttcggca ccgaagcccg ctcgctgaag attttcgtcc
     5161 agcaagttgt cataaaaagt gttataaccc accagccagc tgccgcgcgc ccagcgttgc
     5221 ccaacgccca cattgctcac cagcccatca tcctgctgag taagaccaag ctggctccag
     5281 gtgagataac gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa
     5341 tgtccttcgt tatccacttt gacgtcaaca ctggcatttc cccacgctga tagccaggac
     5401 tctacgtgct gattaacctg ttgactaagc gcatcgcgga cttttcccaa tgcgaaagct
     5461 tttgcctgct cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc
     5521 acaatttcag caaagtgttt ttccccatca tgattttcag gtgccaagcc taaatccggc
     5581 agaccatcat tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg
     5641 ttagctgtac cgcctgccgc cagcaagagt aaataaaacg gaataatgcg aggaacgaaa
     5701 cggctcaaaa cgtcggtaac ttccggtaaa aaatgaaaaa ccatagccct aaagaataac
     5761 gattactggc aacttttgtc gtattttctc gcgctttcag gaaaatcctt aaaaatgtga
     5821 ccgattggtc agacgctgca gaagcagtaa gatagaagat gagtgtctaa ttccctgaat
     5881 acaggagtaa ataatgcaaa aaatcgtgat cgttgccaat ggcgcgcctt acgggagcga
     5941 atccttgttt aacagcttgc ggctggccat tgcgttacga gagcaggaga acaatctgga
     6001 tctgcgtctg ttcctgatgt ctgatgcggt cacagccggg ttgcgcgggc aaaaaccagg
     6061 ggaaggctac aacattcagc aaatgctgga gatccttacc gctcagaatg taccggtgaa
     6121 attgtgcaaa acctgtaccg acgggcgcgg gattagcaca cttcctctga ttgatggggt
     6181 ggaaatcggt acgcttgtgg aactggcgca atggacgctg tcagccgata aagtgctcac
     6241 attttaatgt ttgagggggc gtctgacgcc ccacgatgag ggaagtttac tcaggcgaat
     6301 cccggccgta acacaccatc cggataattc tccgccagca gtttttttac cgataccagt
     6361 aaatcattca gcccatcgtc ctgcattccc agtttgataa gctcctgttc cagtgaaaac
     6421 aggtattgtg cgttggttcc cagtggaccg ctcgccgctg caatcaacgg cgcgatgacc
     6481 tgagcgcgag tgtcagattc atattctgga tgtcgcgggt ccataataaa cacaatggcg
     6541 tttactgtgc gtccatcatc aagatcaagc tgacaccagg ttggcagata acagccggta
     6601 atcatctcgc gcttccacaa cagggttagt tcctgctcca gcgtctcttc tggcagtcga
     6661 taggcgacgc ctgtggtgcg tccgccctct ttcagtgcaa gcatccgtcc cggctggtgc
     6721 gcagtcccgc gcccggcggt caggcgcagg caaaacgcgc gatgccatcc aaccagtgta
     6781 ccggtgcacg actcggtaaa ctccagtgcc ggattccaca tcaacgatcc ataaccgaag
     6841 atccacaccg gtccctcatc aggtcgacaa gccagcgtcg ccgccagcga agccgcccgt
     6901 tgctctgctg accataagag tgattcttca atggcaccaa atgccgtttt acaatcggca
     6961 ttcatcaaga aatcacgcgt tatcacctta cacctccgcc actgcatact tccctgcgac
     7021 aactttttgg tgtcttccgg acatttttac tgctcatttt gaaaccacta acgggacaat
     7081 atgcaatcca cggaccactt gcaaggcaac accgcacgaa tagctttaac taagctaccg
     7141 gttttacgat tttttatgcc atttatcatc atcccctttg gcatattcat gcttcacagc
     7201 agcccaggcc actttatgcg cggtttcttc gcgactggcg tcatcacgcc gatcttcttt
     7261 atctttatat tgatcccatg cgctgttaaa cgcttcttta tagatatcct gggcatgaga
     7321 cggaagcacg tgctttacgc tttccggcag atcgcttttc gttttatacg gcatcgttga
     7381 ctccttttac aaagttgaac attaagtgtg gtcaacaata tccactcttg ccaaaacata
     7441 agaattttca tgtcagactc ttcttaagaa acgctaaaac aaacattgtc taaacgcatt
     7501 gcttattttt atggcccgat gagtaaaaaa tcgacagcgg ggtaaaaatt cgtaaaatgt
     7561 taccgatatc cagacacttt ctgttatttt tcctacctat tttttacatt ttcgataaat
     7621 tcatctgcaa agggataacc ataatgtcaa atgctcaaga ggcggtaaaa acccgccaca
     7681 aggagacttc gcttattttc ccggttctgg cgctggtagt gctgttcctg tggggaagca
     7741 gccagacact accagtggtc attggcatca atcttcttgc tcttattggt attttaagta
     7801 gcgcctttag tgttgtccgt catgcggacg tattagccca tcgcctggga gaaccttacg
     7861 gttcgcttat tcttagcctt tcagtggtta ttcttgaagt cagtttgatt tcagctttaa
     7921 tggcaaccgg cgacgccgcg ccaacgctaa tgcgtgatac gctctattca atcattatga
     7981 ttgttaccgg tgggctggtt ggcttttcat tattgttggg cggacgcaag tttgccaccc
     8041 aatatatgaa tctgtttggt atcaagcagt atttaattgc gctgttcccc ctggcgataa
     8101 tcgtactggt atttccaatg gctctgcctg cggcgaattt ttcaaccggt caggcgttac
     8161 tggtagcatt aatttctgcg gcaatgtatg gcgtattttt gctgatccag accaaaacgc
     8221 atcaaagttt atttgtctac gagcacgaag atgacagtga tgatgacgac ccgcaccacg
     8281 gtaaaccgtc tgcccatagc agcctgtggc atgctatctg gttgattgtc catctgattg
     8341 ccgttattgc ggtgaccaaa atgaacgcca gcccgctgga gacattgctc gacagcatga
     8401 atgcccctgt cgcctttact ggcttcctgg tggctctgtt gattctgtcg ccggaaggtt
     8461 taggtgcgtt aaaagcagtg ttgaacaacc aggtccagcg cgcgatgaat ctgttctttg
     8521 gttcagtgtt agcaaccatt tcgcttaccg tacctgtcgt cacgctaatt gcctttatga
     8581 cgggtaacga attgcagttt gcacttggtg cgccagaaat ggtggtgatg gtggcctctt
     8641 tagtgctgtg ccatatctcc ttctccaccg gacgtactaa cgtgctcaat ggcgcagcgc
     8701 atctcgcact gtttgccgcc tatttgatga cgatatttgc ctgacagtta tatctcctcc
     8761 gatgacagaa atcgggggag ataatataaa ttcctgctta ttgtatttct tcatactacc
     8821 tgaacaggtt gttaatcaga attgcgagcc tgtaccagaa cctgcacgaa accactgcaa
     8881 cagctgttga aaagccggaa tgtactcttt attgcaaaca tttcagggat gaagtgaaga
     8941 tgaattgaga cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg
     9001 gggctttatc cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt
     9061 aaaacctgac aacagaacgg gggctaatct tgaaccatca acaattcaag aatagccgca
     9121 aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc atgattttct
     9181 ggcacgacct ggcagcaccg atcctggcgg gaattattac cgcagcgatt gtcagctggt
     9241 ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc
     9301 gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt ctttatctct tccacaagta
     9361 aatattggca acccgagctg gaagcatcat ccagagcact catgaaacga ctgtaacagc
     9421 tgttgaaaaa ccggaatgca ctcttcattg catacatttc agggatgaag tgaagattaa
     9481 ttgagtcgta gtatccacac caagccggcg ttatccggtg aggcgcaatg ttgcgggggc
     9541 tctatccctg gtggcattgg ttgctggaaa gagaaaaccc ccgcacgttg agaggtataa
     9601 cctgacaaca gaacgggggc taatcttgaa ccagaacaat tcaagaatag ccgcaaaatg
     9661 ttgtcattac aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgac tttctggcac
     9721 gacctggcag caccgatcct ggcgggaatt attaccgcag cgattgtcag ctggtggcgt
     9781 aaccggaagt aaggtgaaaa cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac
     9841 cggccgcagg agtaacagcc tgcggccttt tttgtatccg gccccatgaa aaaagcctta
     9901 acgcagaacg ctaaggcttt agttttcaag caaaaaagat tacttgctgg tatccagttc
     9961 ttcgaaacct ttcaccagat catcaatcgc tttcatctgc ttgaggaacg gttccagttt
    10021 agccagcggc agcgcggatg gaccatcaca tttcgcatgt tccggatccg gatgcgcttc
    10081 aataaacagc cccgccagac ctaccgccat accggcacgt gccagctcag ccacctgagc
    10141 acgacgaccg ccggaagctg cgccaaacgg atcgcggcat tgcagtgcgt gggtcacgtc
    10201 gaaaatcacc ggtgaattac cagacacttt cttcatgatg ctgaagccca gcatatcaac
    10261 aaccaggttg tcatagccga agttagcacc gcgatcgcaa agaatcactt tttcgttgcc
    10321 gccttctttg aatttatcaa cgatattacc catctgtccc gggctgacaa actgcggttt
    10381 cttgacgtta attaccgcac cggttttcgc catggcttca accaggtcag tctggcgagc
    10441 aagaaacgcc ggcaactgaa tcacatccac gacatccgca acgggctgtg cctgacttgg
    10501 ttcgtgaacg tcggtgataa ttttcacgcc aaaagtctgc ttcaactcct ggaagatttt
    10561 catcccttct tccaggcccg gtccacgata agagtggatg gaggagcggt tggctttgtc
    10621 aaaagaggct ttgaacacgt aagggatacc cagtttctgg gtcacggtta cgtagtgctc
    10681 gcaaatgcgc atcgccagat cgcgagattc cagcacgttc ataccgccaa acagtacgaa
    10741 cggcaggtca tttgctacgt tgatgtcgcc aatgctaacc actttttgtt tcataggatc
    10801 gccttaatct tgagtaaaat gtcgattaat taatgcagca caatatgttt atgcgcgatg
    10861 ttatttattt gcgcacggat catttcgctg atcgggtctt ccggacactg ttcaacgaaa
    10921 taacttaaat cgttcaacgc aacgtgttcg caatccagtt gcgcataaat caacccgcga
    10981 tcgcgaattt catagggatc ttccgggttg aattgtaata aagcttcgct ggtacgtagc
    11041 gccagctcca tctgattttc ttccatcaac gaagctttga gtgtatccag caatttgcgg
    11101 attacctcaa tgttatcagc ttcatcaagg tcttcataaa acagttccgc agacgggctg
    11161 atatttccct ttaaccatac gtccagcata tgttcgctta acgattcacc gttaaaagga
    11221 ttaatcagcc aaatttcgcc atccggacat tcaatgcgca atatcagctg cgtagggaaa
    11281 atcaccggca gcagcggcaa atcgagacga ttcgcgaccc ataataaaac ggcacctaat
    11341 gataccgcac tgccctgtcg attctttaac acctggtcca gccataatgc atcggaaaga
    11401 cgataaacgc cgcgtgaggc tttaaatccc cagtcgccgt aaaacagcgc gatcagtttt
    11461 tccagctgct cttctaaagg cagaagctgg ctgatttctt ccttcgccag actaacgaga
    11521 cgctccagtt cgtcgtaaac atcttgcgag ggaaaatcgc ggcggattgc ttcgcaagcc
    11581 aggatcatgc cttcgcacaa tggcgcttta ttaaattcga aatcagctaa cgatctcatg
    11641 acttacccca gtaacggtac ttttgtggtg gcgagtttaa tgatgatgta cagcaccacc
    11701 aacgccagcg ggaaggcaat aatgcgcgcc tgctgactgt gcatacgacg ataatcgagt
    11761 gcaataaaac ccaaaacgat ataaatgata actccaaaca gcttttcagt cagccactgt
    11821 gcctgcccgg aaaatggcag gatgtgcgct ttagccatca acgcaatgcc acttaacaat
    11881 aacagcgtat cgacaaccgg cggcacaatg cgcgtccagc gcgcaaatgc ctgaggatgc
    11941 ttctgataac gtagccagaa acgtaaggtt aatagcccta cagaaagcgc aatgctaatg
    12001 agatgaacgc taagcagtgt agaaaaaatt gtcattgata atagcggccg agcgttacgc
    12061 gctcgttatc accatagtcc cgacaggttt cgacgtcatg atatcccgcg tggataaatg
    12121 cctgtcgcac cgcttcgccc tgctgccaac catgttccag aagcagaaag ccgccggata
    12181 ccagcgcgtt acgcgactgt tcgatgatat gcacgatgtc tgccattcca ctgtctgccg
    12241 caaccagcgc agtgagcggc tcaaagcgga catcgccttg ttgaagatgt gggtcctgct
    12301 cgtcaatata cggcggatta ctgacaatca tcgcaaactg ctgcccggct agcgcgctaa
    12361 accagtcgct ttgcagaata tggatgtttt tgatcgccag attctgggca ttacgttgcg
    12421 ccagagagac agcatctggc atacgatcta cagcggtaat ttcgcagtcc gggcgctcgc
    12481 tagccagcgc cagcgcaatc gccccggtac ccgtcccgag atcgagaata cggcaaggtt
    12541 gttcaggcaa ccgcgccagt gcctgctcca ccagacactc cgtatccggg cgcggaatta
    12601 aggtcgctgg cgaaacaaat aacggcaacg accagaattc tcgcaccccg gttaaatgag
    12661 caatgggttc accatcgcga cgacgtgtca gtagcgcatc aagttgctga cattgttcgt
    12721 cagtcagctg cgtttcacca aaggcgagaa taaaagtacg ccctttgccg gtaacatgct
    12781 ccagcaggat ttcagcatca cgccgcgggc tttcgctcgc ctgaagttgg cttattgctt
    12841 cacgtaacca gtgttgatat tccattattc ctgctcggac aacgccgcca gttggtcggc
    12901 ctgatgttcc tggataatcg gttcaatcag catatccagc ttaccttcca tcacttcatc
    12961 caggcggtag agcgtcaggt tgatgcggtg atcggtaacg cgcccctgcg ggaagttgta
    13021 agtacggtta cggtcgctgc gatcgccact ccccagcagg ttacgacggg tagacgcttc
    13081 ggcctgttgc cgttttgcca tttctgcagc gtggatgcga gcaccgagta cagaaagtgc
    13141 tttagctttg tttttatgtt gtgaacgttc gtcctgacat tcaacaacaa tcccggtcgg
    13201 caagtgagta atacgaattg ccgaatcggt ggtgttaacg tgctgaccac ccgcccctga
    13261 cgagcggaaa gtatcaatgc gcaaatctgc cgggttgatg tccggcagtt ctgcgtcagg
    13321 cagttctggc attaccgcaa cggtacaagc agaagtatga atacgcccct gcgattccgt
    13381 agcaggaaca cgttgcacgc gatgaccgcc agattcgaat ttcaaacgac catatacacc
    13441 atcaccgcta attttggcga tgatctcttt ataaccacca tgttcaccct cgctggcgct
    13501 catgatttct acccgccagc ggcgggcttc ggcataacgg ctgtacatac ggaacagatc
    13561 gcctgcgaac agcgccgctt cgtcgccgcc ggttccggca cggacttcga ggaaggcgtt
    13621 acgttcgtca tcaggatctt ttggtagtaa cagaacctgt aattgctgtt ccagttgctc
    13681 gcttttttct ttagcttcac gcagttcatc ctgcgccatc tcacgcattt caggatcgtc
    13741 gagcatcatc tgtgcggttt cgatatcttc ctgaacctgt tgccagtcgg taaaacagcg
    13801 cgaaacatca cttaactgcg catattcgcg tgataatgcg cgaaaacgtt cctggtcggc
    13861 gatagtttgc gcgtcaccca gcaacgcctg aacttcttca tggcgttcat gcagggcttc
    13921 cagtttggca acgatagaag gcttcatagg cgtaaatgca ccctgtaaaa aagaaaatga
    13981 tgtactgcta ctccagcccg aggctgtcgc gcagaatatt caggcgttcg ttatccccgt
    14041 cacgggcggc ctgttgaagt gatttcgttg gcgcatggat caggcggtta gtcagtttcc
    14101 atgccagatc ctgcataatg gcttgtgcgt cgccgccctg ctcaagggcc gctaacgctt
    14161 tggcggttaa ctcatcgcga acttgctctg cctggctgcg atactcgcga atggtttcgc
    14221 tggcgctttg tgcacgcagc cacgccataa attcgctggt ttcctgagcg acaatagttt
    14281 ccgcctcaac cgctgcggct ttacgctgcg ccaggttgtg cgaaatgatg ctttgcagat
    14341 cgtccacgct ataaagataa gcattcgcca gtttgccaac ttccggctca acatcgcgcg
    14401 gaacggcaat atccaccaac agcattggtt ggttgcgacg gctttttaat gcgcgctcca
    14461 ccatgccttt cccgataatc ggtaacgggc tggcggtgga actaatgatg atatcggctt
    14521 cgcgcagacg ctcgtcgata tcgctcaggg caatcacttc cgcgccgact tcatctgcca
    14581 gaatttgggc acgttcgcga gtgcggttgg cgataatcat cttctgtact ttgtgttcgc
    14641 gcagatgacg cgccaccagc tcgatggttt cgcccgcacc caccagcagt accgtcaccg
    14701 tagagagcga ttcaaagatc tgccgcgcca gcgtacaagc cgcaaaagcg acagacacag
    14761 cactggcacc gatatctgtt tcagtgcgaa cacgtttcgc tacagagaaa gatttctgga
    14821 acatgcgttc cagttcgctg gctttcatat gacctttttg cgaatcagca aaggcttttt
    14881 ttacctgacc gaggatctgc ggctccccca gaaccagtga atccaggccg ctggcaacac
    14941 gcattaaatg gctaaccgcg tcgttatcct gatgccagta gaggcttttg cgcaggtctt
    15001 cttcattaag attgtgataa tcgcaaagcc agcggattaa cgcctcttgc aggttatcct
    15061 gctcttcaac gctaagataa agttccgtgc gattgcacgt cgacagcacc acgccgccct
    15121 gcaccatcgg ctgcgcaagc aggctgtcaa gcgcctgatc gagcttatcc ggcgaaaacg
    15181 atacacgttc tcgcagcgat acaggtgccg ttttatggtt gataccgagt gctaaaaggg
    15241 tcatgtctgc gggaaataat accaacgttg atagggttag tctgcttgca tcatacagga
    15301 tgcgtaagat caataaaaga gagcgccccc ttttggagta attgccgaaa gccgttagat
    15361 tctggcaatt aagacaactt gaacatagac gatagcggac ggtaacgcta gcattaaggg
    15421 ttataactgc aacgtatctc aaggacttgt catcactatg cccctgcccg attttcgtct
    15481 tatccgccta ctaccgctgg ctgctcttgt gctcactgcc tgttccgtta ccacgcccaa
    15541 aggtcctggc aaaagcccgg attcgccaca atggcgtcag catcagcaag acgtgcgcaa
    15601 tcttaatcag tatcaaactc gcggcgcgtt cgcttatatt tctgaccaac aaaaagtgta
    15661 cgcccgtttc ttctggcagc aaactggcca ggatcgctac cgtctgctgc tgactaaccc
    15721 attgggcagc acggaactgg agctgaatgc tcaaccgggt aacgtgcagt tagtcgacaa
    15781 taaaggccag cgttataccg ccgatgacgc cgaagagatg attggcaaat tgaccggaat
    15841 gccaattccg ctcaacagct tgcgccagtg gattttaggt ttaccgggtg atgcaaccga
    15901 ctacaaactg gacgaccagt accgcctgag cgaaatcacc tacagccaga atggcaaaaa
    15961 ctggaaagtt gtttacggtg gttatgacac aaaaactcaa cctgcgatgc cagccaatat
    16021 ggaactcacc gacggtggtc aacgcatcaa gttaaaaatg gataactgga tagtgaaata
    16081 atgcggacac agtggccctc tccggcaaaa cttaatctgt ttttatacat taccggtcag
    16141 cgtgcggatg gttaccacac gctgcaaacg ctgtttcagt ttcttgatta cggcgacacc
    16201 atcagcattg agctttgtga cgatggggat attcgtctgt taacgcccgt tgaaggcgtg
    16261 gaacatgaag ataacctgat cgttcgcgca gcacggttgt tgatgaaaac tgcggcagac
    16321 agcgggcgtc ttccgacggg aagcggtgcg gatatcagca ttgacaagcg tttgccgatg
    16381 ggcggcggtc tcggcggtgg ttcatccaat gccgcgacgg tcctggtggc attaaatcat
    16441 ctctggcaat gcgggctaag catggatgag ctggcggaaa tggggctgac gctgggcgca
    16501 gatgttcctg tctttgttcg ggggcatgcc gcgtttgccg aaggcgttgg tgaaatacta
    16561 acgccggtgg atccgccaga gaagtggtat ctggtggcgc accctggtgt aagtattccg
    16621 acaccggtga tttttaaaga tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa
    16681 acgttgctaa aatgtgaatt cagcaatgat tgcgaggtta tcgcaagaaa acgttttcgc
    16741 gaggttgatg cggtgctttc ctggctgtta gaatacgccc cgtcgcgcct gactgggaca
    16801 ggggcctgtg tctttgctga atttgataca gagtctgaag cccgccaggt gctagagcaa
    16861 gccccggaat ggctcaatgg ctttgtggcg aaaggcgtta atctttcccc attgcacaga
    16921 gccatgcttt aagccgggca agctgagttt cggtgacaac gtcaccttgt tccagacgtt
    16981 gcatcgcgct ctttaataca ccgcctggaa agaatcatgc ctggcccgca cagttttcgg
    17041 cagattcttt ccaccaatgg acgcatgcct gaggttcttc tcgtgcctga tatgaagctt
    17101 tttgctggta acgccacccc ggaactagca caacgtattg ccaaccgcct gtacacttca
    17161 ctcggcgacg ccgctgtagg tcgctttagc gacggcgaag tcagcgtaca aattaacgaa
    17221 aatgtacgcg gtggtgatat tttcatcatc cagtccactt gtgcccctac taacgacaac
    17281 ctgatggaat tagtcgttat ggttgatgcc ctgcgtcgtg cttccgcagg tcgtatcacc
    17341 gctgttatcc cctactttgg ctatgcgcgc caggaccgtc gcgtccgttc cgctcgtgta
    17401 ccaatcactg cgaaagtggt tgcagacttc ctctccagcg tcggtgttga ccgtgtgctg
    17461 acagtggatc tgcacgctga acagattcag ggtttcttcg acgttccggt tgataacgta
    17521 tttggtagcc cgatcctgct ggaagacatg ctgcagctga atctggataa ccctattgtg
    17581 gtttctccgg acatcggcgg cgttgtgcgt gcccgcgcta tcgctaagct gctgaacgat
    17641 accgatatgg caatcatcga caaacgtcgt ccgcgtgcga acgtttccca ggtgatgcat
    17701 atcatcggtg acgttgcagg tcgtgactgc gtactggtcg atgatatgat cgatactggc
    17761 ggtacgctgt gtaaagctgc tgaagctctg aaagaacgtg gtgctaaacg tgtatttgcg
    17821 tacgcgactc acccgatctt ctctggcaac gcggcgaaca acctgcgtaa ctctgtaatt
    17881 gatgaagtcg ttgtctgcga taccattccg ctgagcgatg aaatcaaatc actgccgaac
    17941 gtgcgtactc tgaccctgtc aggtatgctg gccgaagcga ttcgtcgtat cagcaacgaa
    18001 gaatcgatct ctgccatgtt cgaacactaa tcgaacccgg ctcaaagacc cgctgcggcg
    18061 ggtttttttg tctgtaatat ccatttgtat gacctatgcc tccttcacct gccatttagt
    18121 tgacagatga tgcgctcacg gatgaaacat tattgtgaac aaaatatttt cctcacatgt
    18181 gatgcctttc cgcgctctga tcgacgcttg ctggaaagaa aaatatactg ccgcacggtt
    18241 tacccgtgac ctgattgccg ggataaccgt cgggattatt gctatcccgc tggcaatggc
    18301 gttggctatt ggtagtggtg tggcacccca gtacggttta tataccgcag ctgttgcggg
    18361 gattgtcatt gctctgacgg gtgggtcacg ctttagcgtt tccggtccga ctgcggcatt
    18421 tgtggtaatt ctctatcccg tttcgcaaca gtttggactg gcaggactgc tggtcgcgac
    18481 actcatgtca ggtgtctttt tgatcctgat ggggctggca cgttttggac gcttgattga
    18541 atatattccg gtttccgtga ccttaggttt cacctcgggt atcgggatca ccatcggtac
    18601 catgcagatt aaagattttc tcggtctgca aatggcccat gtcccggagc attatctaca
    18661 aaaagtcggc gcattattta tggcgttgcc gaccattaat gtgggtgatg ctgccattgg
    18721 cattgtgacg cttggcattc tggttttctg gccgcgtctg ggcattcgtt tgcctggtca
    18781 ccttccggcc ttgctggctg gttgcgcggt gatggggatt gttaacctgc tcggcgggca
    18841 tgttgctacc atcggatcgc aattccacta cgttctggca gatggttctc agggtaacgg
    18901 tattccgcaa ctgctgccgc aactggtact accgtgggac ctgcccaatt cagaatttac
    18961 cctcacctgg gattctattc gcacactgct gcctgcggca ttctcaatgg caatgctcgg
    19021 cgcaatcgaa tctctgctct gcgccgtggt actggatggt atgaccggaa cgaaacacaa
    19081 agcgaacagc gaactggttg gacagggact ggggaatatc atcgccccgt tctttggtgg
    19141 tattaccgct acagctgcca tcgcgcgttc tgccgctaac gtccgtgccg gggcaacttc
    19201 ccctatctcg gcggtgatcc actctattct ggttattctt gccctgctgg tactggcacc
    19261 gctgctctcc tggctgccgc tttccgccat ggctgccctg ctgttgatgg tggcgtggaa
    19321 catgagtgaa gcgcacaaag tggtcgactt gctgcgtcat gcgccgaaag atgacatcat
    19381 cgtcatgctg ctgtgcatgt cgctgactgt gctgtttgat atggttattg ccatcagcgt
    19441 ggggatcgtg ctggcatcgc tactgtttat gcgtcgtatc gcacgtatga ctcgcctggc
    19501 accggtagtc gtagacgttc cagacgatgt tctggttctg cgcgttattg gtccgctgtt
    19561 ttttgctgcc gctgaaggct tgttcacgga cctggagtca cgtcttgaag gcaaacggat
    19621 tgtgattctg aagtgggatg ccgttccggt acttgatgct ggtggtcttg atgcgttcca
    19681 gcgttttgtg aagcgtctgc ccgaaggatg tgaactgcgc gtgtgcaacc tggaattcca
    19741 gccactgcgc acgatggctc gcgcaggcat tcaaccgatc ccgggacgcc tcgcattctt
    19801 cccgaatcgt cgcgcggcga tggcggattt ataagtacga gattgaccag tcagcgcaaa
    19861 ctgactggtc agcaaactgc attatttgtt agctatgacg gcgattatcg ctacggcggc
    19921 aacgtttgtc atagtggcga acccaccagt aacggtcgca cacctgttca tgcccgccaa
    19981 cacgggcacc cgccagccag agaatggcac cgacgaaaat acttagcact gcaccatgtg
    20041 cgaaatactg gggcatatta aactgtggta actggttgag gattgaatac cccacgccga
    20101 ccaccattac caccagaccc aaccccatga gcacgttacc gagtaacgaa gcgtttttgc
    20161 gtttcatatg tcacctccgg aactttctgg gttgtaacag ggaatacccc tcttccttat
    20221 gtgtaaagta tagacaacac cctgtgggtt atgtgcgggc gtgatcacaa ttacaaccct
    20281 tatttcaaca aaactttaca aataaacgcc tgaacacctt gactttctaa tagtccactc
    20341 aggcaattat tgcgttatct gcatttgata cgcagttttt tgtcaagcgg ggccgcaacc
    20401 agtaaactac gcgccagtta tgtacacact caggacaaaa aaacgtgacg attaaattga
    20461 ttgtcggcct ggcgaacccc ggcgctgaat acgccgcaac gcgacataat gctggtgcct
    20521 ggttcgttga cttactggca gagcgattgc gcgctccgct gcgcgaagag gctaaattct
    20581 ttggttatac ttcgcgagtc acccttggag gcgaagatgt ccgcctgtta gtcccgacta
    20641 catttatgaa tctcagcggc aaagccgttg cggcgatggc cagttttttc cgcattaatc
    20701 cggacgaaat tctggtggcc cacgacgaac tggatctgcc tcttggcgtc gccaaattta
    20761 agttgggcgg tggccatggt ggtcacaatg gactgaaaga catcatcagt aaattgggta
    20821 ataaccctaa ctttcaccgt ttacgcatcg gaatcggtca tccgggcgat aaaaataaag
    20881 ttgttggttt tgtattaggc aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca
    20941 tcgacgaagc ggcgcgttgt actgaaatgt ggtttacaga tggcttgacc aaggcaacga
    21001 accgattgca cgcctttaaa gcacaataag tcgttgtctg cggcattttt gccgagtgcc
    21061 gtgtataata ggcaaagtta tttccatttc tgcaatctgt tagcaataac aggttgatta
    21121 ttaagatatt aaggtgattt aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa
    21181 cgtcgggaaa tctaccctgt tcaacgcgct gaccaaagcc ggtattgaag cggccaactt
    21241 tccattctgc accatcgagc cgaacacagg cgtcgtacca atgcctgacc ctcgcctgga
    21301 tcaactggct gaaatcgtaa aaccgcagcg tacgcttccc acgaccatgg aatttgtcga
    21361 tatcgccggt ctggtaaaag gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac
    21421 caacatccgt gaaactgaag cgatcggtca cgttgttcgc tgctttgaaa atgacaacat
    21481 cattcacgtt tccggcaaag ttaacccggc tgatgatatt gaagttatca acaccgaact
    21541 ggcgctggcg gacctcgaca cctgcgaacg tgcgattcat cgcgtacaga agaaagccaa
    21601 aggtggcgat aaagacgcga aagccgagct ggcggtactg gaaaaatgcc tgccgcagct
    21661 ggaaaacgca ggtatgctgc gcgcgctgga tttaagcgct gaagagaaag cggctattcg
    21721 ttacctgagc ttcctgacgc tgaaaccaac aatgtacatc gccaacgtca acgaagacgg
    21781 ttttgaaaac aacccgtatc ttgaccaggt gcgtgaaatc gcggcgaaag aaggttctgt
    21841 tgtggttccg gtttgtgctg ctgttgaagc agacattgcc gaactggacg acgaagaacg
    21901 tgacgagttt atgcaggagc ttggactgga agagccgggc ctgaaccgtg tgatccgtgc
    21961 cggttataag ctgctgaacc tgcaaactta cttcaccgct ggggtgaaag aagtacgtgc
    22021 atggaccatt ccggttggtg caaccgcgcc gcaggccgct ggcaaaatcc atactgattt
    22081 tgaaaaaggc tttatccgtg cacaaactat ttcgttcgaa gatttcatca cttacaaagg
    22141 tgaacaaggc gcgaaagaag caggcaaaat gcgtgcagaa ggcaaagatt acatcgttaa
    22201 agatggcgat gtaatgaact tcctgttcaa tgtctaatta atttgttgtc tcatgaggtt
    22261 tcgctaaatc tcatgaagag cgcaaaagta tataaaatcc acgcaattgc gtggattttt
    22321 cattttaccg agtcctaacc attcttataa catcctacac ttttgtgctt attgcctata
    22381 gtgaaccacc agaataatta aagacataag tgttcagatg agggatatct cattagcaat
    22441 gagataacgt aagtttgtgt tccactgcac atcagacacc ttttacagct gatcaatatt
    22501 gatcacgctc caatatgctg gcgagaaaag gacttgcctg aagggcaata tccagcagat
    22561 aaagcccccg aataacgtga ttacgtgcga tcctggcaag atgaaaatga taaccagtag
    22621 cacgataata acgataagcc agaaaatcca ttgatcaaaa atgaatatcg atgttttttg
    22681 ctttttctta ttagtaatgc aaaaatggct catattgttt ttgttatgca gatacatcaa
    22741 gcgaaaatcc cataactaat taggattatt accaaaaata ttcaccaaat tatttaaccg
    22801 ctgtttgttg taatgttttt gtcatattca tagtaacgaa ttggaataag taacgacaac
    22861 ttagggaata actcgatcta aactcacctt tgtaaggagg atgttaaata tatattaatt
    22921 cctgtgttcc tgattttgcc cggtaataga gaattattct cagagcagct catcatgatt
    22981 gcaccataca aatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca
    23041 aaatccatag acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca
    23101 atgggaatac caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tccattcaac
    23161 tgataaaata tggcgctatt ggtttgaatg caattatctt ttcccccctg ctgatagctg
    23221 ctgatttagg aagtcaaaat ggcaccaata ttactattag tgatggtgac agaattacag
    23281 gagataccgc cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc
    23341 ctggcaatat caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg
    23401 caaagggaat cattattcag ggcaagaaca gctccctgac agctaaccga ctcacagtag
    23461 atgttgttgg tcaaacctct gccatcggca ttaatttaat tggtgactat acccatgctg
    23521 acttaggcac aggcagcacc attaagagta acgatgacgg catcattatt ggacatagct
    23581 caacactaac agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca
    23641 atgactatgg caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta
    23701 caggtgttta tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg
    23761 cgacagacct gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa
    23821 actctgttgt cgatctcgga acaaacagta ccattaaaac caatggcgat aatgcacacg
    23881 gcctctggag ctttggccag gtgagcgcga atgcactcac tgttgatgta actggagccg
    23941 tggcaaatgg cgtcgaagtt cgtggtggta caaccactat cggtgctgat agccatattt
    24001 cttccgcgca gggcggtggc ctcgtaacca gtggttcaga cgcgacaatc aatttttctg
    24061 gcacggcagc gcaacgaaac agcatctttt ccagcggttc ttatggtgcc tcagcccaga
    24121 cggcaacggc tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatgacagtc
    24181 tggcgctggg tttgtgggcg ctcagcggcg gtagaataac cggagacagt ttggctatca
    24241 ccggcgcggc aggagccagg gggatttatg ccatgaccaa cagccagatc gatctcacga
    24301 gcgatctggt cattgatatg agtacacccg accagatggc catcgcaacg caacatgacg
    24361 atggttatgc cgccagccgc atcaacgctt cgggtcgtat gcttatcaac ggtagcgttc
    24421 tctccaaagg tgggctaatc aatctggata tgcacccagg gtcggtttgg acaggttctt
    24481 ccctcagcga taatgtcaat ggcgggaaac tggacgttac aatgaataac agcgtctgga
    24541 acgtaacaag taattctaat ctcgacacgc tggcgctgag ccattcaact gtcgattttg
    24601 ccagccacac gtcaactgcc ggcacattca ccacattaaa cgtagagaac ctgagcggta
    24661 acagtacctt tattatgcgt gctgatgttg ttggcgaggg taatggcgtt aataataaag
    24721 gggatttatt gaatatcagc gggagtagtg ctggtaatca cgtattggct atccgtaacc
    24781 agggcagcga ggccacaacg ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg
    24841 cggcctcgtt cagcgcgtct tctcaggttg agttgggagg atatctgtat gatgtgcgta
    24901 aaaatggcac taactgggag ctctacgctt ccgggacaat tccggagccg actccaaatc
    24961 ctgagcccac accagctccc gctcagcctc ccatagtcaa ccccgatcct acgcctaacc
    25021 ccactcccac acctaaaccc accacgaccg cagatgctgg cggcaattat ctcaatgtcg
    25081 gttacttatt gaactatgtt gaaaaccgta cgctgatgca acggatgggt gacctgcgaa
    25141 atcagagtaa agacggtaat atctggttgc gcagttatgg gggaagcctg gactcctttg
    25201 ccagtggcaa actgagcggc tttgacatgg gttacagcgg tatccagttt ggtggggata
    25261 aacgtctctc tgatgaaatg ccgttgtatg tcggtctgta tattggctca acacatgcat
    25321 cgccggacta tagcggaggc gacggtaccg cacgttcaga ctacatggga atgtacgcca
    25381 gttacatggc acacagcggt ttttacagcg atctcgttgt aaaagcatca cgccagaaaa
    25441 atagtttcca cgtactggac agtcagaaca acggcgttaa tgccaacggc actgcgaatg
    25501 gaatgagtat cgccctggaa gccgggcaga ggttcaacct gacccctact ggttatgggt
    25561 tctatataga gccgcaaacc cagctcacat acagccacca gaatgagatg gctatgaagg
    25621 cgagtaatgg cctcaatata catctgaatc actacgaatc gctgctgggg cgtgccagca
    25681 tgatactggg atatgacatc accgcaggca acagccagct gaatgtctat gtgaagactg
    25741 gcgctatccg cgagttttca ggggataccg aatatctgtt gaacaactcc cgggagaagt
    25801 acagtttcaa aggtaatggc tggaataacg gcgtgggagt cagtgcacag tataacaaac
    25861 agcacacatt ctatctcgaa gcggattaca cgcagggtaa cctctttgat cagaagcaag
    25921 tcaacggagg atatcgcttc agcttttaag tgggggatag acaagccctt aaacgatgct
    25981 gaaaattgtt agcgtgcgcc ggaatgaaat acagatcact ctgatgtatc ataaatcgtt
    26041 tctgtctttc atacccggcg cttaaactgc cctgcatcaa tgccatgttg cttcattttc
    26101 cgccacagcg tagtgcggcc gattccaagt aaagccgaca tttcctgaat gcgaccgcct
    26161 gtgacctggg ctgcgttaat aattgcctct ttttcaactt ccgcaaatga cagactggtg
    26221 gaaaggcggg tggcgctgac atcatctgtc gcctgctcgg taaacagatg ttcaggtaaa
    26281 tcactgacgc gaatgcgccc gttatcacta ctcagagcaa gattctcgat gacgctgtaa
    26341 agttcaaaat cgttgcctgg ccatgcacaa gaaaccaggc gagcgagggc atcgtcgtca
    26401 attttcagac gcgtagagaa gcgtttttca agactgcgta atttgttatt caccagcgcc
    26461 ggaatgctgc cacgccgcat acgcagaggc gggatggtaa tttcaaatgc atgcagcgcg
    26521 taatacagct ggcgactaaa gcgattttgt tccaccagca ttgccaggtc cgcggtcgtt
    26581 gtggcaatca ctttgacatc aatgggtatt aaacgccgcg catccagtcg cgtgataacc
    26641 ccctgcttga taacctgaag caaagcagac tgtaactcca ccgccagata ttcaatcttt
    26701 tcaagaaaca gcgtgccacc gtgcgccagt tccagccgac tcagacggcc attttcattg
    26761 tccgtgcgat cgccgccaat aaattcttcc gccagcgccg catcaccata taactcacaa
    26821 ttgacggcga tataaggccc cgctgcacgc tcactttcat tatgaatggc ctgacttagc
    26881 agtgccttac ccacgccctc ttctccacaa agcaggacag gaaagctact gcgcgccgcc
    26941 tggcgaccaa aatgaatcaa gcggcgggtt tgcggatcct cttgtggcat atgagcgaaa
    27001 gtatggctga cttttcctaa ttgactggtc attaactgcc gcatctgttc cacaggatgg
    27061 agcaacaaaa taaagctggt tccctgcgtt tctattatcg gtttaagggt tatcaccgca
    27121 tcaataaact ggtgctgact ttcaaaggtt gcttctacgt gtttgagcgg atgtgcctgt
    27181 tttattgctt gttgcaatac ggcgggtaac gttaagagtt cagtgattgc ccttccctga
    27241 cttgccgtcg cgtcaaggcg caagacccgc gccgcctggg cgttgataaa ttgcaaatta
    27301 ccctgctcgt cccagctaat cacgccatca tccatacttt ctaacagggc attaagttga
    27361 tttaaatgac ggttagtttc agcgagcaaa ctgtccgtca gcagtaaatt accgacctct
    27421 cgggcgattg ccaacgtcaa cggcaaatca gctgcggtag tttgctcaac cggacacgcc
    27481 agcgctattg ttcccgtcaa tcggcccttg ctgtcaaaca acggcgttgc acaaaaggcc
    27541 cagttccaga gtgcctgttt gaaatgttga tcggccatcg ttttcacggc ctgaccagag
    27601 atagccgcta acgatagcgc acaagtacca ataattccct cggcgcaata cgtgccgtca
    27661 ttgaatccca gtgtacttag ttgctgcaag gtttgcggat cgccattacg gctgagaatg
    27721 caggcggttt catcgaggat aagcaacgca cactctcgcg gtgccatata ttcccaggca
    27781 tcttccagcg cagcctgccc gagcgtcagc atcgctttct tacgccgata aatagacgca
    27841 aatgtcacgc cctgggcctg gtgtggtacg ttccatgtct cccgtttcat cagcttattg
    27901 catctctccc aggaggttgc aattaaggga gatatgcccc gaccatcgtt gttaaaagcg
    27961 ccactcatat cccgcatccc ttatgacaaa aggtgttccg gctcgcaatt ttaagtatta
    28021 atacacgtaa aagtttcaat atggaacatt cattaaagca tatgtttcac tatgaaacat
    28081 aatttgggct aattttcttt tcccttgcct gatgcacaat ggattcatcg gcagtcagtg
    28141 gtcgccgtgt cgttgaacat catccatgcc ctaccgtaat tgctggagca aaataatgaa
    28201 aaaattgatc aatgatgtgc aagacgtact ggacgaacaa ctggcaggac tagcgaaagc
    28261 gcatccatcg ctgacactgc atcaggatcc ggtgtatgtc acccgagctg atgcccccgt
    28321 tgcaggtaaa gtcgccctgc tgtcgggtgg cggcagcgga cacgagccga tgcattgtgg
    28381 ttatatcggt caggggatgc tttcgggggc ctgtccgggc gaaattttca cctcaccgac
    28441 gcccgataaa atctttgaat gcgccatgca aattgatggc ggcgaaggtg tactgttgat
    28501 tatcaaaaat tacaccggcg atattcttaa cttcgaaaca gcaaccgagt tactgcacga
    28561 tagcggcgta aaagtgacca ctgtggtcat tgatgacgac gttgcagtaa aagacagtct
    28621 ttataccgcc gggcggcgcg gcgttgccaa caccgtatta attgaaaaac tcgtaggcgc
    28681 agcggcggag catggcgact cactggacgc ctgtgcggaa ctggggcgta agttgaataa
    28741 tcaaggccac tcaataggta ttgctctcgg tgcctgtacc gttcctgccg cgggcaaacc
    28801 ttcttttacc ctggcggaca atgagatgga gtttggcgtc ggcattcatg gtgagccagg
    28861 tattgaccgc cgtcccttct cttcccttga tcaaaccgtc gatgaaatgt tcgacaccct
    28921 gctggaaaat ggctcatacc atcgcacttt gcgtttctgg gattatcaac aaggcagctg
    28981 gcaggaagaa ccacaaacca aacaaccgct ccagtctggc gatcgggtga ttgcgctggt
    29041 taacaatctt ggcgcaactc cgctttctga gctatacggc gtctataacc gcctgaccac
    29101 acgttgccag caagcaggat tgactatcga acgtaattta attggcgcgt actgcacctc
    29161 actggatatg accggtttct caatcacctt actgaaagtt gatgacgaaa cgctggcact
    29221 ctgggacgcc ccggtccaca ccccggccct taactggggt aaataaggag atagctatgt
    29281 cactgagcag aactcaaatt gttaactggc tcactcgttg tggcgatatt ttcagcaccg
    29341 agagcgagta tcttaccgga ctggatcgcg aaattggcga cgctgaccac gggctaaata
    29401 tgaaccgagg ctttagcaaa gtggtggaaa aactccctgc tatcgcagat aaagatatcg
    29461 gtttcattct caagaatacc ggtatgacgc tactttccag cgtcggtggt gccagtggtc
    29521 cgctgttcgg taccttcttt atccgcgccg cacaggcgac ccaggcacgg caaagcctga
    29581 cgctggaaga gctttatcag atgttccgcg atggcgcgga cggcgtaatc agtcgcggta
    29641 aagccgaacc tggcgataaa accatgtgtg atgtctgggt gccggtggtg gaatcgttac
    29701 gtcagtcctg cgagcaaaat ctctctgttc cggcggcgct cgacgctgcc agtagcatcg
    29761 ccgaatccgc tgcacaaagt acgattacga tacaagcccg caaaggccgc gccagttatc
    29821 tcggtgaacg cagtattggt caccaggacc ccggtgcgac ctcggtgatg tttatgatgc
    29881 aaatgttggc gttagccgca aaagagtaag gaattggtga tggtaaacct ggtcatagtt
    29941 tcacatagca gccgactggg agaaggtgtc ggtgaactag cccgtcagat gttaatgagt
    30001 gatagttgta aaatcgccat tgccgcggga attgacgatc cacaaaatcc cattggtacc
    30061 gatgccgtca aagtgatgga ggccatcgaa tctgttgccg ataccgacca tgtgctggtc
    30121 atgatggata taggaagcgc attattgagt gctgaaactg cgctggaatt gctggctccc
    30181 gagatcgccg caaaagtacg tttgtgtgct gcaccgttgg tcgaaggtac actggcagca
    30241 acggtcagcg cggcctcggg ggcggagatc gacaaagtta tctttgacgc catgcatgcg
    30301 ctggaagcca aacgtgaaca actgggttta ccctcctcca acactgaaat ctctgacaca
    30361 tgtcctccgt acgatgaaga agcccgttct ctggcggtgg tcataaaaaa ccgtaacggc
    30421 ctgcatgtac gtccggcctc ccggctggtt tataccttat cgacatttaa tgccgatatg
    30481 ttgctggaga aaaacggcaa atgcgtcaca ccggagagta ttaaccagat tgcgttacta
    30541 caagttcgct ataacgatac gctgcgcctg attgcgaaag ggccagaagc tgaagaggca
    30601 ctgatcgctt tccgtcagct ggctgaagat aactttggtg aaacggagga agttgctcca
    30661 cctattctgc gtcccgttcc gcctgtttcg ggtaaagcct tttattatca accggtttta
    30721 tgtacggtac aggcaaaatc aaccctgacc gtggatgaag aacaagaacg attacgccag
    30781 gccattgact tcacgttatt agatctgatg acgttaacag cgaaagcaga agccagcggg
    30841 cttgacgata ttgccgcaat cttttctggt caccatacac tgttagatga tccggaactg
    30901 ctggcggcgg caagcgaact ccttcagcat gaacattgca cggcagaata tgcctggcag
    30961 caagttctta aagaacttag ccagcaatac cagcaactgg atgatgaata tctacaggct
    31021 cgctatattg atgtggacga tcttctgcat cgcaccctgg tccacctgac ccaaacgaaa
    31081 gaagaactcc cgcagtttaa ctcgccaact attctactgg cggagaacat ctatccctcc
    31141 acaatactgc aactggatcc ggcggttgta aaaggtatct gccttagcgc cggaagtccg
    31201 ctatcccaca gcgccctgat cgcccgtgaa ctggggattg gctggatttg ccagcagggt
    31261 gagaaactgt atgcgataca accagaagaa acgctaacgc tggacgttaa aacgcaacgt
    31321 ttcagccgtc agggttaaac caccgttcgg atggcatcat tctgatgtca tccggcaatt
    31381 tactctgcac ggatgttttt aactacctgt tatatttttc accaatttgt gatgattttt
    31441 cttcgctaat cgcgaaccca acgccgctgg ctggctaaca aacagacgtt atttgatcac
    31501 tctatttcgc cgttatttat ccctttaatt cccttttcta aaatgcctga ccgttcgcag
    31561 aatgagattt cgatcatgca gctagtgcga tcctgaacta aggttttctg atacttgaac
    31621 accgttttat tcggtttcgc caaaggagaa tgattgatga aatcccccgc accttctcgc
    31681 ccgcaaaaaa tggcgttaat tccagcctgc atctttttgt gttttgctgc gctatcggtg
    31741 caggcagaag aaacatcggt aacgccacag ccgcctgata ttttattagg gccgctgttt
    31801 aatgatgtgc aaaacgccaa actttttccg gaccaaaaaa cctttgccga tgccgtgccg
    31861 aacagcgatc cgctgatgat ccttgctgat tatcggatgc agcaaaacca gagcggattt
    31921 gatctgcgcc atttcgttaa cgtcaatttc accctgccaa aagagggcga gaaatatgtt
    31981 ccgccagagg ggcagtcact gcgcgaacat attgacggac tttggccggt attaacgcgt
    32041 tctaccgaaa acaccgaaaa atgggattct ctgttaccgc tgccggaacc ttatgtcgtg
    32101 ccgggcggac gttttcgcga ggtatattac tgggacagtt acttcaccat gttaggactt
    32161 gccgaaagcg gtcactggga taaagtcgcg gatatggtgg ccaattttgc tcatgaaata
    32221 gacacttacg gtcatattcc caacggcaac cgcagttact atttaagtcg ctcgcagccg
    32281 cccttctttg ccctgatggt agagttactg gcgcagcatg aaggcgatgc cgcgttgaag
    32341 caatacctgc cgcaaatgca aaaagaatat gcttactgga tggacggtgt tgagaacctg
    32401 caagccggac aacaggaaaa acgcgttgta aaacttcagg atggtaccct tctcaaccgc
    32461 tactgggacg atcgcgatac gccacgacca gagtcatggg tggaagatat tgccaccgcc
    32521 aaaagcaatc cgaatcgacc tgccactgaa atttaccgcg acctgcgctc tgccgctgcg
    32581 tctggctggg atttcagctc gcgctggatg gacaacccgc agcagttaaa taccttacgc
    32641 accaccagca tcgtaccggt cgatctgaac agcctgatgt ttaaaatgga aaaaatcctc
    32701 gcccgcgcca gcaaagctgc cggagataac gcgatggcaa accagtacga aacgctggca
    32761 aatgcccgtc aaaaagggat cgaaaaatac ctgtggaacg atcaacaagg ctggtatgcc
    32821 gattacgacc tgaaaagtca taaagtgcgc aatcagttaa ccgcggccgc cctgttcccg
    32881 ctgtacgtca atgcggcagc gaaagatcgc gccagcaaaa tggcgacggc gacgaaaaca
    32941 catctgctgc aacccggcgg cctgaacacc acgtcggtaa aaagtggaca gcaatgggat
    33001 gcgccaaatg gctgggctcc cctgcaatgg gtggcgacag aagggttgca aaactacggg
    33061 cagaacgagg tggcgatgga cattagctgg cacttcctga ccaatgttca gcacacttat
    33121 gaccgggaga agaagctggt ggaaaaatat gatgtcagta ccaccggaac aggtggtggc
    33181 ggtggcgaat atccattaca ggatggcttt ggctggacca atggcgtgac gctgaaaatg
    33241 ctggatttga tctgcccgaa agagcaaccg tgtgacaatg ttccggcgac gcgtccactc
    33301 agtgaatcaa caacacagcc ggtaaaacaa aaagaggcgg aacctacgcc ttaactttgc
    33361 tgtatatcga gcaatgccat tatgtgcata aaattggcat tgctcttttt tcgccacgaa
    33421 atgcccc
//
LOCUS       DAYPHN010000035        32956 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000035 DAYPHN010000000
VERSION     DAYPHN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.035"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..828
                     /locus_tag="ACG0BW_001634"
     CDS             <1..828
                     /locus_tag="ACG0BW_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFK7258609.1"
                     /translation="NRRDVVLESFQPYLVINQGAVTPCTFLGDQTGDRFSNICGDQFI
                     IDLLKRIMSINKNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPK
                     YKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIINGKKFK
                     TSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVL
                     GLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNKNGLMPLIRRRTMLQL"
     gene            1018..1131
                     /gene="yhiY"
                     /locus_tag="ACG0BW_001635"
     CDS             1018..1131
                     /gene="yhiY"
                     /locus_tag="ACG0BW_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFK7258610.1"
                     /translation="MMTTLLPVFTKPSTLALNALRAGRICRFLLIPDGRIR"
     gene            1194..2261
                     /gene="yhiI"
                     /locus_tag="ACG0BW_001636"
     CDS             1194..2261
                     /gene="yhiI"
                     /locus_tag="ACG0BW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFK7258611.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAIA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGI
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2258..4993
                     /gene="rbbA"
                     /locus_tag="ACG0BW_001637"
     CDS             2258..4993
                     /gene="rbbA"
                     /locus_tag="ACG0BW_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFK7258612.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSSEAEAPPVVHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4993..6117
                     /gene="yhhJ"
                     /locus_tag="ACG0BW_001638"
     CDS             4993..6117
                     /gene="yhhJ"
                     /locus_tag="ACG0BW_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7258613.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPISIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQTVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(6208..7059)
                     /locus_tag="ACG0BW_001639"
     CDS             complement(6208..7059)
                     /locus_tag="ACG0BW_001639"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFK7258614.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVK"
     gene            complement(7090..7359)
                     /locus_tag="ACG0BW_001640"
     CDS             complement(7090..7359)
                     /locus_tag="ACG0BW_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFK7258615.1"
                     /translation="MLSKTVEVRNSTGLHARPAACLVKAAKKFSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCEGDDENEAIQTLTTLFRDRFGEAE"
     gene            complement(7349..8857)
                     /locus_tag="ACG0BW_001641"
     CDS             complement(7349..8857)
                     /locus_tag="ACG0BW_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFK7258616.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GNVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTVGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWSLDDIPLFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATQNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(8850..10208)
                     /locus_tag="ACG0BW_001642"
     CDS             complement(8850..10208)
                     /locus_tag="ACG0BW_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFK7258617.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10285..10566)
                     /locus_tag="ACG0BW_001643"
     CDS             complement(10285..10566)
                     /locus_tag="ACG0BW_001643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFK7258618.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(10563..11036)
                     /locus_tag="ACG0BW_001644"
     CDS             complement(10563..11036)
                     /locus_tag="ACG0BW_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFK7258619.1"
                     /translation="MQGIQFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKYVRHNAIAVGILPEPVEFADMGGDPDPVHVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMRALLTMETTEIHTVILNKMKERGEI"
     gene            complement(11061..11807)
                     /locus_tag="ACG0BW_001645"
     CDS             complement(11061..11807)
                     /locus_tag="ACG0BW_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFK7258620.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRENIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKQGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSKRKIRYSESRYAARLIGNERGHFLDICEDVPVLHLEQLVFF
                     SRELPVEFGNVWLKGNKYYLGTVLQRRELS"
     gene            complement(12006..12407)
                     /gene="nikR"
                     /locus_tag="ACG0BW_001646"
     CDS             complement(12006..12407)
                     /gene="nikR"
                     /locus_tag="ACG0BW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFK7258621.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(12412..13218)
                     /gene="nikE"
                     /locus_tag="ACG0BW_001647"
     CDS             complement(12412..13218)
                     /gene="nikE"
                     /locus_tag="ACG0BW_001647"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFK7258622.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAYPVRRRATEKV"
     gene            complement(13215..13979)
                     /gene="nikD"
                     /locus_tag="ACG0BW_001648"
     CDS             complement(13215..13979)
                     /gene="nikD"
                     /locus_tag="ACG0BW_001648"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFK7258623.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGSSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13979..14812)
                     /gene="nikC"
                     /locus_tag="ACG0BW_001649"
     CDS             complement(13979..14812)
                     /gene="nikC"
                     /locus_tag="ACG0BW_001649"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFK7258624.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14809..15753)
                     /gene="nikB"
                     /locus_tag="ACG0BW_001650"
     CDS             complement(14809..15753)
                     /gene="nikB"
                     /locus_tag="ACG0BW_001650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFK7258625.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLTSTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(15753..17327)
                     /gene="nikA"
                     /locus_tag="ACG0BW_001651"
     CDS             complement(15753..17327)
                     /gene="nikA"
                     /locus_tag="ACG0BW_001651"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7258626.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQVGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(17438..18025)
                     /gene="acpT"
                     /locus_tag="ACG0BW_001652"
     CDS             complement(17438..18025)
                     /gene="acpT"
                     /locus_tag="ACG0BW_001652"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFK7258627.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALRDQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLSADSVQWIDSVN"
     gene            complement(18027..19256)
                     /locus_tag="ACG0BW_001653"
     CDS             complement(18027..19256)
                     /locus_tag="ACG0BW_001653"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFK7258628.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEKSLKIAGLSASDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19253..19984)
                     /locus_tag="ACG0BW_001654"
     CDS             complement(19253..19984)
                     /locus_tag="ACG0BW_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFK7258629.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQNGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGTRQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(19984..20448)
                     /locus_tag="ACG0BW_001655"
     CDS             complement(19984..20448)
                     /locus_tag="ACG0BW_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFK7258630.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEEVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMVLGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINNGTATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(20445..21614)
                     /locus_tag="ACG0BW_001656"
     CDS             complement(20445..21614)
                     /locus_tag="ACG0BW_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFK7258631.1"
                     /translation="MIYISAVGMINALGNNLDDIAANLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPVIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALADAQLTPDEVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILER
                     DLPLPAQDFSLSPRDPTLPPCGIIEQPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(21616..22200)
                     /locus_tag="ACG0BW_001657"
     CDS             complement(21616..22200)
                     /locus_tag="ACG0BW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFK7258632.1"
                     /translation="MIKPTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(22197..24515)
                     /locus_tag="ACG0BW_001658"
     CDS             complement(22197..24515)
                     /locus_tag="ACG0BW_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFK7258633.1"
                     /translation="MTNANVLPPSKRPALLWGIVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLVDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISNLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKFTASLENA
                     KKEGLISNYRTIPLNSLARQEEDLHLLKTAAPTVTKALQNAGLAAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLKNGESGVLVPVEGVKSSALLQEIASDYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLALGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(24484..25089)
                     /locus_tag="ACG0BW_001659"
     CDS             complement(24484..25089)
                     /locus_tag="ACG0BW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFK7258634.1"
                     /translation="MKFLPLLALLISPFVSALNLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(25086..25508)
                     /locus_tag="ACG0BW_001660"
     CDS             complement(25086..25508)
                     /locus_tag="ACG0BW_001660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFK7258635.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(25512..27188)
                     /locus_tag="ACG0BW_001661"
     CDS             complement(25512..27188)
                     /locus_tag="ACG0BW_001661"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFK7258636.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALNVSDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLLPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEVQDKE"
     gene            complement(27179..27532)
                     /locus_tag="ACG0BW_001662"
     CDS             complement(27179..27532)
                     /locus_tag="ACG0BW_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFK7258637.1"
                     /translation="MKPHEIERHQAQANHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALTLLAPGWRFHSIQNIKFQSPLLPDNRVTLTLNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(27519..28880)
                     /locus_tag="ACG0BW_001663"
     CDS             complement(27519..28880)
                     /locus_tag="ACG0BW_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFK7258638.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLDESSWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     DLNWQGSLLLVASSPQVATQSFTFAAIAPDAYIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVKHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPFPGMHFQAEGEAFRLFSPLIAEDNGLLLDDLLQFSQDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     ARLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(28877..29458)
                     /locus_tag="ACG0BW_001664"
     CDS             complement(28877..29458)
                     /locus_tag="ACG0BW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258639.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLIALL
                     LLLRLRQTRRRAGPLQAVTQIVAVVGIALCVASFLLKTHQLLLFYPVVVNAIMLAVFG
                     GSLWSTMPIVERLARLQEPDLPEKGVRYTRRVTQIWCGFFIINGGIALFTALYGDMSL
                     WTAWNGMIAYFLMGTLMAGEWLVRRQVIKRDRS"
     gene            complement(29463..29714)
                     /locus_tag="ACG0BW_001665"
     CDS             complement(29463..29714)
                     /locus_tag="ACG0BW_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFK7258640.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(29726..29983)
                     /locus_tag="ACG0BW_001666"
     CDS             complement(29726..29983)
                     /locus_tag="ACG0BW_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFK7258641.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIETDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(29958..30779)
                     /locus_tag="ACG0BW_001667"
     CDS             complement(29958..30779)
                     /locus_tag="ACG0BW_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFK7258642.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVEVHGRVEINKFYDATSQEPALAARQLNRHLLLLLQQGCLPLSGINNASALSGN"
     gene            complement(30776..31498)
                     /locus_tag="ACG0BW_001668"
     CDS             complement(30776..31498)
                     /locus_tag="ACG0BW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFK7258643.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIESGDDWQCETQSAIAGNETSLPQSMLFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            complement(31539..32597)
                     /locus_tag="ACG0BW_001669"
     CDS             complement(31539..32597)
                     /locus_tag="ACG0BW_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFK7258644.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYAASVLLDMGLSGRIITCKEGMYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(32666..32875)
                     /locus_tag="ACG0BW_001670"
                     /pseudo
     CDS             complement(32666..32875)
                     /locus_tag="ACG0BW_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32891..>32956)
                     /locus_tag="ACG0BW_001671"
     CDS             complement(32891..>32956)
                     /locus_tag="ACG0BW_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFK7258645.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         7629 a         8873 c         9018 g         7436 t
ORIGIN      
        1 aacagaagag atgtggtttt agaaagtttt cagccatatt tagttattaa tcagggcgca
       61 gtgacgccat gtacgtttct gggtgaccaa accggcgatc gatttagtaa tatctgtggc
      121 gatcaattta tcatcgattt gttaaaacgc ataatgagta ttaataaaaa tgttcatgta
      181 ttagctggta atcatgaaac gaattgcaat ggtaactata tgcagaactt cacgcgtatg
      241 aaaccacttg atgaggatac ctacgctggg ataaaagatt atcctgtatg tttctatgac
      301 cccaaataca aaataatggc aaatcatcat gggataactt ttgatgatca gcgaaaacgt
      361 tacattatag gaccgataac agtctctatt gatgaaatga ccaatgctct cgatccggtc
      421 gaattagcgg caattattaa taagaagcat catgccatca ttaatggtaa aaagtttaaa
      481 actagccggg ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac
      541 aggcctaaac ttgaagcact attagcatgc tcacaaatgt taggtataaa tcaagttgtt
      601 gcacataacg gaaatggtgg gagagaacgc ataggtgaaa cggggacagt tttaggtctt
      661 aacgcccgtg acagtaaaca tgctggaaga atgttcagca tgcataattg ccaaattaac
      721 cctggtgcag ggcctgagat aaccactccc tggaagtctt accagcatga aaaaaacaaa
      781 aatggactta tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac
      841 atgaatatta atgttactat ctggttgaga ctgttgataa aacgtaataa ggaatgctct
      901 gttcctgcaa ctgaacaggc gtgaaacgct atccaacagg atggataccg ttttgtcagc
      961 cagttttata gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg
     1021 atgacaacat tactacccgt tttcactaag ccttccacgt tggcgctcaa tgctctgcgc
     1081 gctggtcgta tttgccgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc
     1141 ggcggcagtt gtaacacgcg tcgtttgatt acagggatta tggcgggtcg attatggata
     1201 agagtaagcg ccatctggcg tggtgggttg tcgggttact ggcggtggcc gctatcgtgg
     1261 cgtggtggct gttgcgcccg gcaggtgtgc cggaaggttt tgccgtcagt aatgggcgca
     1321 ttgaagcgac ggaagtggat atcgccagta aaattgccgg gcgtatcgac accattctgg
     1381 tgaaagaagg ccagtttgtt cgcgaaggcg aagtgctggc gaagatggat actcgcgtgt
     1441 tgcaggaaca gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccattgccg
     1501 ccgcgcaggc tttgctggag caacggcaaa gcgaaactcg tgccgcacag tcgctggtca
     1561 atcaacgcca ggccgaactg gactccgttg ccaaacgtca tacgcgttcc cgctcgctcg
     1621 cccaacgagg ggctatttct gcgcaacagc tggatgacga ccgcgccgcc gctgagagcg
     1681 cccgagctgc gctggaatca gcaaaagccc aggtttcggc ttctaaagcc gctatagaag
     1741 cggcacgcac caatatcatt caggcgcaaa ctcgcgtcga agccgcgcaa gccactgaac
     1801 ggcgtattgc cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc
     1861 agtatcgggt tgccgagcca ggcgaagtgc tggcggcagg cggtcgggtg ctgaatatgg
     1921 tcgatctcag cgacgtctat atgaccttct tcctgccaac cgaacaggcg ggcacgctga
     1981 aactgggcgg tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attccggcga
     2041 ccatcagttt tgtcgccagc gtcgcccagt tcacgccaaa aactgtcgaa accagcgatg
     2101 aacggctgaa actaatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc
     2161 atctggaata tgtcaaaacc ggtttgccgg gcatagcgtg ggtgcgggtg aatgaagaac
     2221 ttccgtggcc tgacgacctg gtggtgaggt tgccgcaatg acgcatctgg aactggttcc
     2281 cgtcccgcct gtcgcgcaac tggcaggcgt gagccagcat tatggaaaaa ccgttgcgct
     2341 gaacaatatc actctcgata ttccggcccg ctgcatggtc gggctgattg gcccggacgg
     2401 cgtcgggaag tcgagcttgt tgtcgttgat ttccggtgcc cgcgtcattg agcagggcaa
     2461 tgtgatggtg ctgggcggcg atatgcgcga cccgaagcat cgacgcgacg tttgcccgcg
     2521 catcgcctgg atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga
     2581 aaacgtcgat tttttcgccc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat
     2641 taatgagctg ctgaccagca ccgggttagc accgtttcgc gatcgtcctg caggtaaact
     2701 ctccggcggg atgaagcaaa aactggggct gtgctgtgcg ttaatccacg acccggaatt
     2761 gttgattctt gatgagccaa caacgggggt tgacccgctc tcccgcgccc agttctggga
     2821 tctgatcgac agtattcgcc agcggcagag caatatgagc gtgctggtcg ccaccgccta
     2881 catggaagag gccgaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc
     2941 aaccggcagc gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaagcatt
     3001 tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccata
     3061 tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc
     3121 cttcgttgcc gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct
     3181 tggttcgaac ggctgcggta aatccaccac catgaaaatg cttaccggat tgctgcccgc
     3241 cagcgaaggt gaggcgtggc tgttcgggca accggttgat ccaaaagata tcgatactcg
     3301 ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca
     3361 aaacctggag ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcgcgagt
     3421 ggctgaaatg agcgagcggt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt
     3481 accgctcggc attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggagat
     3541 gttaatcctc gatgagccta cttctggtgt cgatccggtg gcgagggata tgttctggca
     3601 gttgatggtc gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat
     3661 gaacgaagcg gaacgttgcg accgtatctc actgatgcac gccggaaaag tgctcgccag
     3721 cggtacaccg caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat
     3781 cgcctatttg caggaagcgg cagggcagag cagcgaagcc gaagcgccgc ccgtggtaca
     3841 cgacgccacc cacgcgccgc gtcagggatt tagcctgcgc cgtctgttta gctacagccg
     3901 ccgcgaagcg ctggaactgc ggcgcgatcc ggtacgttcg acgctggcgc tgatgggaac
     3961 ggtgatcctg atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt
     4021 tgcggtgctc gaccgcgacc agaccgtcag tagccaggcg tggacgctca atctctccgg
     4081 ttcccgttac tttatcgaac agccgccgct caccagttat gacgagcttg atcgtcggat
     4141 gcgtgcgggc gatatcacgg tggcgattga gatccctccc aatttcgggc gcgatatcgc
     4201 gcgtggtacg cctgtggaac tcggtgtctg gattgacggt gcgatgccga gccgtgccga
     4261 aacggtaaaa ggttacgtgc aggccatgca ccagagctgg ctacaggatg tggcgagccg
     4321 acaatccaca ccctccagcc aaagcgggct gatgaacatt gagacgcgct atcgctataa
     4381 cccggacgta aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat
     4441 gattccgtca atgctaagcg cccttagcgt ggtgcgggaa aaagagttgg ggtcgattat
     4501 caacctttac gtgactccca ccacccgtag cgaatttttg cttggcaaac agctgccgta
     4561 cattgcgctg gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg
     4621 cgtaccgcat aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat
     4681 tgccaccgga atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt
     4741 cggtacggcg attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt
     4801 agcttcgctg gaaggtccag gacgttggat cggcgaggtt tacccgacca gtcattttct
     4861 gactatcgcc cgcgggacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt
     4921 tatcccgctg ctgatagcca taccgctggt gatgggctta agcatcctgc tgctgaaaaa
     4981 acaggaggga tgatgcgcca tttacgtaat atttttaatc tgggtatcaa agagttgcgc
     5041 agtctgctcg gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtatcggtg
     5101 tattcgtcag cgaccgttac gccaggatcg ttgaacctcg cgccgatctc tattgccgat
     5161 atggatcaat cgcagctgtc gaaccggatc gttaacagct tctatcgccc gtggtttttg
     5221 ccaccggaga tgatcaccgc cgatgagatg gatgccggac tggacgccgg gcgttatacc
     5281 tttgcgataa atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccagat
     5341 attcaggtga acgtcgatgc cacgcgcatg agccaggcat tcactggcaa cggttatatc
     5401 cagaatatta tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taatagcgaa
     5461 ccgttggtat cgctggaaac ccggatgcgc tttaacccga acctcgatcc cgcgtggttt
     5521 ggcggggtga tggcgatcat caacaacatt accatgctgg cgattgtgct gaccggatcg
     5581 gcgctgatcc gcgagcgcga acacggcacg gtggaacact tactggtgat gccgataacg
     5641 ccgtttgaga tcatgatggc gaaggtctgg tcgatggggt tggtggtgct ggtggtgtcg
     5701 ggattatcgc tggtactgat ggtgaaaggc gtgctgggcg taccgattga aggctcgatc
     5761 ccgctgttta tgctgggcgt ggcgctcagt ctgtttgcca ctacgtcgat cggcattttt
     5821 atggggacga tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgcta
     5881 ccgctgcaaa tgctttccgg tggatccacg ccgcgcgaaa gtatgccgca gacggtgcag
     5941 gacattatgc tgaccatgcc gacgacacac tttgtcagcc tcgcgcaggc cattctctac
     6001 cgtggtgccg gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc
     6061 gcatttttca ctatcgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaaata
     6121 cagttgcccg atgcgtaagc ttatagggtc tacgcatcgg tacaatttat tgaattgtag
     6181 ttgttttacg ccgcatccgg cgaggattta cttaaccaac cgatgcccct cattcatcat
     6241 caggattttg ctatacacat cttcctcgac ggcgtttttt gctgatgcca tcactcgtgc
     6301 cagattcgcc tcgttgtgat tattcacata cgctttgccg acggcggtga taaacgcttt
     6361 gcgcagatcg ctggcgatat ttactttagc gacgcgataa ttaacaaagc tgcgcaatat
     6421 ctccggggcg ataccggagc cgccgtgaat aaccagcggc accgggcaga cttcggcaat
     6481 gcgcttcagc agggggatat cgatccgcgg tatatcgtcc agaccatgta cgttgccaat
     6541 agaaactgcc agcatgtcgc aaccggtgcg ctcgacaaag gtcttcacct tttccggttc
     6601 cgttttgcaa tcggcttcgc tgacgtgatc atcttctttg ccgagaatcg cccccagctc
     6661 cgcttcaacc ggtacgccat aggatttaca gaaatcgaca gcttcctggg tgaaagcgat
     6721 attctcttca aaagagaatg cggcaccgtc aatcatgacc gaggtaaagc ccgcgcgcac
     6781 cgcctgttta acatcctcct gagttttccc gtgatccaga tgaagactga ccggaacatc
     6841 cattttatcg gcgtgacgtt tcacgatttc ataaatgtaa tcgtatcccg agagttgtgc
     6901 attggtgggc gcaatctgaa tgaagttagg caatcccgaa cgctcaatag cgtttaaaat
     6961 tgaaattgtc gtctccagat tggtggtatt aaacgcgccc gctaataccc gtttttcctg
     7021 aatgcggtcg agcagtatcc gtccattaac taatggcatt tgaaaacctc atcttttaat
     7081 atcagttaat tattcagctt caccgaagcg gtcacgaaat aaggtggtga gagtttgtat
     7141 tgcctcattt tcatcatcgc cttcgcagcg tatttccacc gttttgccgc cttttattcc
     7201 ggcgcgcata atattcatca tgctggtggc attaatatca ttaccctcat aatgcagcgt
     7261 cactttacag ctaaactttt tggcggcttt caccagacac gccgccggac gtgcatgcag
     7321 gccagtactg tttctgacct ccacggtttt actcaacata gtgctcctcc cgatggtgta
     7381 atggcatcac gccttcttgc tgtaatttca actgatacca gcttttccat ttgtgctgat
     7441 aacgctgtaa ttgatggtat ctcgcccgat tcggtaacaa acgttgggct atggtaactt
     7501 gcggcggtgg aatatccggt gcggcagtta ataatgctcc gacagaaacc gcctcgttaa
     7561 atgcaattgc ttcaaccgga cgctgtaaaa tatccgcttt taattgcagc caaaggggat
     7621 tttgggttgc cggacctatc acttttaacg cgctggtgcc aggcacatta aaacaggcga
     7681 agcagttggc aaattccatg gttaatccca gtgagacgct ggcaattaac atgtcgatat
     7741 ttaacgtatc gccaagccca taaaataatc cacgagtatg gcgattttta tagggcgaac
     7801 ccgaaccgcg aagatgtgga ataaagaggg gaatgtcatc gagcgaccag ttccccgcca
     7861 ggtaatccgc atgtccgcga gtaagtgatg cggcgatctc ctcatcggtt agccggaacg
     7921 tgttacgcag ccactccaga gcgaaaccgc ccacgggcag cgacgcaaac agggtgaact
     7981 ggtttgcaag cgaataacag ccgtttgcca gcttgtcacg tttggcctgt tcatccagcg
     8041 tcggttgtgt atccagttgc agaatgcctt ctgtggtccc cgtcgagtta aggatatcgc
     8101 ccggcatcat ctgcaacgct cgcgcaccca ccatatggtc atgtccggcc agcgtcacgc
     8161 tgaccggttg cgagaaccca accgttttgc agagcgactc gctgacccat ccggcgtgtt
     8221 cgcctggctg caccaatggc gcaaatgctg aacagggaac gtgtaacaac gccgccgctt
     8281 tcgctgacca ttcgcctttg cgcacgtcaa gacacagcgt gcggctggct aaggtgatat
     8341 ccgtgcgctg ttcgccggtc agccgccaga gcagtacttc cggcgcatgt agccaacgca
     8401 ggccgcggcg attacgcagt gggtaatgtt ccagcaacca acgcattttg aaggcagagt
     8461 aattgctgtg cagcggtaac ccgcaaatgt cataaagtgc cgcactgtct gcctcgctaa
     8521 gtgtagccag atactcttca ccgcgacggt cataccatgc cagcattggc gtcaggatct
     8581 cgccatgctc gtcaaggaac acgcctgact cgccaaaact ggcaatgctt atccgcctga
     8641 ctggcagcgg cgaagcggcg ttcagttgcg ctatcgcctg gcggacctcc tgccagaggg
     8701 cgtcgatatc gaaatcgaca ttgccctgtg gggagatctg ttttgccgtc acgaatttat
     8761 gcgcgcccaa cgtcgttgcg tccaggcagg aaaagcaggt gactttgcaa ttggtggtgc
     8821 caatatcgat aacgatagct gcgctgttat caggcataaa ccgtgctccg cgtcattcgg
     8881 aaaataccat acagtaccag ggcgataacc ggcaccagac caatggcgat gagattgccg
     8941 ttgaagattt ccagcagcca gaagcggaac gggttaccgc catcaaggaa actggagacc
     9001 tggccgctgg agccttcggc aaatttgaaa ttaacgtctt tcgccattcc ggtaataaac
     9061 ggcgcgattt tagtcgcaat ccacagatcg gcaataatca ccggaatcgc cgccagaacg
     9121 gtgcggaaaa tgttgccacg gctggctaaa gcaatcatcg acgccattac cgccaggttg
     9181 gcaaggtcgc caagcggcag gacttcatta cccggaagga caaacgcgat aaagaccgag
     9241 attggcgtca gcagcagggc ggtggagata atcgctgggt tacctacggc aacggcgata
     9301 tccaggccga tatagagatc gtcacggtca gggtatttgg cattgagata ctttttaatg
     9361 gcttcagaca gcggtaataa accttccatc agaatgcgaa ccatgcgtgg caggatgaac
     9421 atcacaccgc caatgctgat gccaagcagc aagacttttt tgaaatcgta tccggcaatt
     9481 acgccgagca gaatgcccag aatagtacca accatcatcg gttcgccgaa tatgccaaac
     9541 tttttctgta cggtttccgg atcgatatga atacgattga ggccggggat atggtcgatg
     9601 attttgtcga ccaacagacc gaccggaaag aacaccaccg aagagagcgt cggcaaagag
     9661 atcccttcca ggccaaagta tttttgtacc agtggcgcag accagtcggc gagtttaagt
     9721 actaccaccg cagcaatcgc cgctgccagc aaaccaaaga acaggctgcc ggtgctgtaa
     9781 taaaccagcg taccggcaag ggcaaaatgc cagtagttcc acatatctac gtcaacggtg
     9841 cgggttttat ttaacgccag catcgccacg ttaatcagaa tagtcagggg aataacaaac
     9901 ggggcaattg gcgaacccca tgtaatagcg gaaagcggcg gccagcctaa atccaccacc
     9961 ggtaaattaa ttccggtgcg ttcaaccatc gcctgggccg ctggcccgac attgctggta
    10021 agaatatcga aaatggcata aataccgaca aaaccaatgc cgaccgttaa tgacgaacgt
    10081 aaggctttag cgggttttac tttaaaaaag agagcaagaa taaataacac caacggcaac
    10141 attaccgtgg gaccaaatcc caatatatat tgcacaatat tatagagggt atgcgcgata
    10201 tcgttcataa taaacctctt tttcaggcga gagcgcacct cgcgttagcg cctgatggag
    10261 tgggttgaaa ttaattatta ttaattactt tttaaggatc gaaacaattt gcgccagtac
    10321 tttctcttcg ccaataccgg taataatcgg caagccgtta accaccggga cgtcgagttc
    10381 ataaggcact ttagtcgttg agactaccag tgctacgccg tcagtatttc caggtaaaga
    10441 ggcaacgttg gtttgggaat aattgacgtt aagaccgtgc tctttacaat attccatgac
    10501 tttttctgtt accgccgtgg atgtggcaat acccgtggcg cagacaaata aaatggtttg
    10561 actcatattt cacctcgttc tttcattttg tttaagatta ctgtgtgtat ttctgttgtt
    10621 tccatcgtaa gcagggcgcg cataaagggc gtatcctgaa tcatctccat aatccagccc
    10681 agggcattta attgtttatt actttcactt aaggcgagca aaaaaatcac cctgacatgt
    10741 acggggtcag gatcgccgcc catatcggca aactctaccg gttccggaag gatgccgacg
    10801 gcaattgcat tgtgcctgac atatttatgg tccgtgtgcg gaatggcgac gcacggtggt
    10861 tcggtaggca gccccgttgg gtacgtcgcc tcacgtttca gtacgtgatc ggcaaaatca
    10921 gggaccacca gctcagcagc aaccaacggt tgcgctaact gacggatgat ctgttcaacg
    10981 cttaaccctg ccggaagtcg ttgaatataa ttttcctgaa actgtatgcc ttgcatttta
    11041 ttcctcataa aaaagtgtat ttaactcaat tcccgccgtt gcagcacggt gcccagataa
    11101 tatttatttc cttttaacca gacgttgcca aactcaacgg gtaactctcg ggagaaaaag
    11161 actaattgct ccagatgcaa aacgggtaca tcttcacaga tatctaaaaa atgaccgcgt
    11221 tcattaccaa ttaatcgcgc ggcatagcga ctttccgagt aacgaatttt tcttttcgac
    11281 aaactttcta ttgttggaaa taaattgtgt tgattaaaat cgatttccac gatgccgggg
    11341 catagctcaa tattgatacg gttctctatc agcatcgctt tttcatcacc aattgaacgt
    11401 aaacgctcaa ggtaaagaat atcctgaccg ggcgttattc ttaatttctc tgccacataa
    11461 cgattagccg gttcaatccg tgacgtaata acttcagtgg taaagtgtat tttctggctt
    11521 tccagcgatt ccgcgaatga caataatcct tcacctaacg gatatgccac gttctctttt
    11581 ttgacaaatg ttcccttccc ctggacctga atcaacacgc cttgttttac cagttgggcg
    11641 acagcttttt taacggttcc ccgactcact tccagcatat ccataatctg gtattccgaa
    11701 ggaatgcgat cgtcttccac cagatccccg gtataaatat tttctcttat ccactccacc
    11761 acttgttgat agagcggtat ttgtgacgac ttactcaatg atttcatggc gcgcacctga
    11821 acatttacag ttgtctatgg acaatcatgg acaacttcaa gcgtatagta ggtgaacagg
    11881 cgaaaaagag gatcgaagcg tctcacattt taggggacta aatcgtgatc aaaaaaatgt
    11941 gacgtgagtc gatatttggc agaagtctac tgcgccgtca ggaaataacg gcgcgttggg
    12001 aggtgttaat cttccttcgg caggcactgc aaatgcccgt gccgcacgcc gcgctgggcg
    12061 atgacgtcat cggcaaaatg ctgcacgtcg cccatgtcac ctttcaacac ggcgatttcc
    12121 agacagtcgt cgtgattaat gtgcacatgc agcgtggcga ctgagagatc gtggtgatgg
    12181 tgctgggtgg agacaatacg gctggctaag tcgcgttttt cgtgttcata cacatacgac
    12241 agcaccgcga aaccttgcgt gccgtgctgc tgggtagctt cctgcgccag ggcgctacgc
    12301 agaatatcgc ggatagcttc ggaacggttg ttatagccac gacgctggct caggctgtcc
    12361 agcgtctcca gtaaatcgtc atcaagcgtg atggtaactc gttgcataga cttaaacctt
    12421 ttctgtggcg cgacggcgca cggggtatgc gggtaatacc gcgttttgta gcacacgtcc
    12481 ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga tttgcccgtt
    12541 gtccatcacc atcactcgct ggcaaaagcg ttccaccagg cgtaagtcat gagtgatgaa
    12601 caggcaggca gtgccaaact gttgttgtag ctttttcagc aggcgaatga cgcccgcctg
    12661 taacacgaga tcaaggttag aaacggcttc atccagaatc agtagtttcg gttcgaccgc
    12721 cagcgcgcga gccaggcaga cgcgctggag ctgaccgccg cttaactgcg gtgggcgttt
    12781 gtctagaacg ctgtcatcga gatcgaccgc cttcagcatt tcgctggcgc gtgccagttg
    12841 ttccgatttt tttagcgata gcaggtggcg catcggctcg cgcaggatct cgcgcacggt
    12901 tttgcgcgga ttcacggcgc tgatggagtc ctgaaatacc atctgaatat cgcggcggaa
    12961 cgctttacgc tgggcgcgat tgagctttgc caacggttcg ccgcgccagc taatattccc
    13021 ttgtgaggga gactctaagc ctaccagcag ccgcgcgaga gtgctttttc cgcagccgct
    13081 gcgccccagc agagcgacgg tttcgccgct tttcagagtc aaagaaacgt tattcagcac
    13141 cgcctggtgt tgatgtttgc cgctaaatcc accgtgcgca tagtgatggg aaaggtcgga
    13201 gacgttaagt aaagtcatga tgccagctcc ataccgtaga gggcgagatg agcggaaacc
    13261 aggctgcgcg ttaccgcatg tttgggggca ttaaacagcg tttctacatc gccctgttcg
    13321 acaattttac cgtgtgacat caccgccacg tcatccgcca gacgcgccac aacgcccata
    13381 tcatgggtca ccagcagcat tcccggcgct tgtttttgca taatgctttc cagcagatcg
    13441 aggatgcgcg cctgtgctac cacgtcgaga tcggtggtcg gttcatcggc gatgataaac
    13501 ggcgattcac acaacaccgc catcgcaatc atcatgcgct gcaacatgcc gccgctcatc
    13561 tcgaacgggt acagcttcaa cacgcgcgcg gcgttttcca accccaccgc ttctaaagca
    13621 gcggtaagcg tggcgtcatc ggcgggtttc cctaacgcca ggcaggtttc ccgcgcgtgg
    13681 gtgtgcatgg tgtgaagcgg attaaaggcg ctgcgcgggt tctgcatgat ggtggcaatt
    13741 ttgatgccgc gcagggcgca gggagaaacc ggtttgccat cggctaaaat ttcccccgcc
    13801 gtctggcgaa cgcctgcggg cagaatgccc agtgcggcgg cgcaggttaa tgatttcccg
    13861 ctgccgctac tgccgactaa cgccagcacg cgcccgcgtt gcagagttaa cgatacgccg
    13921 tgcaccagcg gttgcgcggc ctgtagcgcg atattacgta gttcaatctg ttgcggcatt
    13981 agtgtgcgtg ctccgtcacc agatgagggt ccagatgatc gcgcagtgcg tcacccacca
    14041 ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt tgcagcggct
    14101 gggtccagat atactggcgc gcgtcgttaa tcatcacgcc ccattcggcg gtaggcgcgg
    14161 tcacgccgag gccgaggaag gacatccccg cgacgtggag catcatatga ccaatatcca
    14221 gcgtcgccag caccagcagc gaggggatca ccgcgcctgc cagatgatcg acaaacaccc
    14281 gcacatggcc cgcgccggaa agccgtgacg ccagcacaaa ctcgcgctgg cgtagcgaaa
    14341 tcaccaggct gcgcaccatg cgcgcatacc acgcccagtg cgacagggcg atggcgataa
    14401 tcacgttggt caacccggtg ccgagtacgc cgaccataaa gaacgacaga atcgaagtcg
    14461 ggaaggtcat aaacatatcc gcgacgcgca tggtggcctg atcaacgcgc ccgccaatca
    14521 gcccggcgct gccgccaata actagcccta atgtcagcac cagcagcagg caggccatta
    14581 ccgagccgag cgacacgcgg gtcgccgcca tcagccgcga gaaaatatcg cgccctaaat
    14641 gatcggtacc caaccagtgc tgcgcatccg gcgaaagcag gcgcgacggc aaatcaatcg
    14701 cctgggggtc atacggcagc caccactggc tggtgagcgc aatcagcgcc agcagggcga
    14761 taatgatcag cgccaggcgt accgaccagc gggaagagag gaaaaagttc acgcgtgggc
    14821 tccttcatga cgacgaatgc gcgggtccag cgcggcgttg agcaaatcga caatcaaatt
    14881 acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg gatagtcacg
    14941 gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga tgttttcgat
    15001 aatcatcgtc ccgccaatca gttcgccaat gtgcatcccc acggcggtga tcatcggcag
    15061 cgaggcattg cgcagaatat gacgacgctc ggtctgtttg tcattcaggc cgcgtagccg
    15121 cgcccaggtg acgtgacgct gaccggcgac gtccagcata ctggcgcgca gcagccgcgc
    15181 gttaatcgcc agcgacataa aggcaatgga aaccgcaggc aaaatgatgt gctgccagcc
    15241 gccgtagccc atcgcgggta gccattgcag atataccgaa aacgccatca ccagcaaaaa
    15301 cgccagccag aagttgggca tcgacacgcc gagaaacgcg atgaaacgga cggcgaaatc
    15361 gggcaagcga tcgcggtggc gtgcagccca gataccgagc ggcacggagg tgagcagaat
    15421 taataccagc gccgcacccg ccagttccag cgtggcgggc aggaagttca gcatatcgtc
    15481 cagcaccggg cgttggctgg cgaatgagat accgaaatca agatgcagcg ccttccatag
    15541 ccaggtgccg tattgcacgt acagcggctg atccagcccc agcatggtgc gggtagaggt
    15601 cagcatctcc ggcgtcggcg gcaggttaga taaacgcaaa taatcaagcg ccgggtcgcc
    15661 ggtgccgagg cgcagcatca aaaaaatgat caccgaggcg gcgagcacca tcgggatcag
    15721 cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt aatctgttcg
    15781 aacggaattt cggtggcgat cggcgcgtag gggatgttac ccagctccgg ttttgatacc
    15841 accatcattg agatgtaact gataggcaga taaaccgcct cgtcatgcag acgggtcaga
    15901 atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg ttgccagcac ttcgccgatc
    15961 tctttatcaa tcagcggctt gtcggctaat ccttgctgtg cctggaaatc agcgtgcgac
    16021 ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca ggtgcggtgg
    16081 aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc ttcttcgcca
    16141 atcagcgaga catctgcgcc aacctggcgc atatcagcct gaatgatttc cgccatcgat
    16201 ttacttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg accatttttc
    16261 tcgcggatgt ctttgcccgc aggcaacgtc caaccggctt tttccagcaa cgctttcgct
    16321 ttctgcggat cgtactggcg cggtttcagg ccgaggttgg cgtagggtac agaaggagca
    16381 aacagggtgt cggcgacctg ctgggtgcca tacaacgcgt tatcaatcag cgattttttg
    16441 tttaccgcgt aattaagagc ttcacgtacg gccagctcgt tggtgggggc tttggcggta
    16501 ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gggtgtggta agccgggttc
    16561 tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta cagcaggtcg
    16621 atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt ccgggatcac gttaaaggtg
    16681 atctttttaa tcgctggctt ttcgccccag tagttttcgt tacggacgaa gacatcgtac
    16741 tgattcagtt tcgattcctg taaaacccac ggcccggtgc caatcggcgc tttaattccg
    16801 ttcatggttt cgtggttttt aaactgcgag ggtgcgataa aacggaaagg acggggcagg
    16861 gccagttctt gcaggaaagg atagtaggcg cttttcaggg tgatttgcag ctcgttttta
    16921 ctgagtgctt tgacatcaac aatctggttt gccagctcca gccaggcgtg acgttgacgg
    16981 ttatcgagca ctgcgcggaa gttttctgcc gccgcctctg catcaaacgg ttcgccgttg
    17041 gagaatttca cgtcatcacg cagggtgaag gtccaggttt tgccatcttc agaatgagtc
    17101 cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac caatggttca
    17161 taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt tagtggcccg
    17221 acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac gataaaagac
    17281 gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtag agaacatggt aaccccaatg
    17341 gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat actgattacc
    17401 tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa tcgatccatt
    17461 gcacactgtc ggcggagagc gtaaaggggg taggggtgca gatcgccagg cttaaatttt
    17521 ctagctgaca atggctgact gacagtgatg agtgataggt gctgtctacg ctgacgattt
    17581 gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag atgcgccaga
    17641 acatttccag ttgctgatcg ggatgcacgg cgtccatctc agcgtgttcc ccgaggctga
    17701 acacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg atatcgcagc
    17761 cgacttcgcc ttcatcgctc aacagcaggg cgatatcgtc accgctatgg cttaagttga
    17821 accatagcgg cgtttccggc gcgaatgccg gtttgccttg ttcgccatag atgatctccg
    17881 gtagcgggga aagcgtgtgc gaaagtaatg cacgccccgc cagccagcgt tcgcgtcgtg
    17941 gaccttgcgg tgcttgatcg cgtaaagccg gtggcagtgg agctgcgctt aaggtcgaaa
    18001 ctttccccag aactatccga tacatatcag ggccaacgtt taatgatgat ggatgtatta
    18061 atgccgccaa aggcaaagtt gttactctgc aaaaactcac aatcaacctt gcgggcttca
    18121 tgcatgatgt aatccagcgc gccacagttc gggtctggtt tattcaggtt tagcgtaggc
    18181 gcaaaccaac cttcgcgcat catttgcaga ctcatccagg cttcgagcgc gccacaggca
    18241 ccaagcgtat gaccaaaata acttttcagc gaggagagcg gcacattatc accgtaaatg
    18301 gttgctgtcg ccagactttc tgccatatca ccacgatcgg tcgccgtacc atgcgccgaa
    18361 atatagccga tatcacttgc acttaacccg gcgattttta acgatttttc catacaatat
    18421 tgcatggtct cacgctgagg ctgggtaata tgtgccgcgt cgcagttagt cgcaaagccg
    18481 acgatttcac cgtaaatggt cgcgccgcgc gctttggcgt gctccagttc ttcaagaatc
    18541 agcgtacctg cgccttcacc aatcaccagc ccgtcgcggt tttcatcgaa cggtgaaggt
    18601 gtggttttcg gcgcatcatt gtgctggctg gtggcgaaaa gtgtatcaaa taccgctgct
    18661 tctgaagggc ataactcttc ggcaccacca gcaaccatca ccgtttgata accgtggcga
    18721 atggcttccc acgcataacc gatcgcctgg ctgccggagg tacaggcgct ggaggtcggg
    18781 atcacccgcc cgcgcaggcc gaaaaacaga ccggtattaa cggcggtggt gtgcggcatc
    18841 atctgtacat aggtggtgcc ggtgatgtta ttggtgtgct tttccgtcag catggtggca
    18901 aattcgctca ccggaccggt actgccggtc gatgaaccgt acgcaatgcc cgtttcgccg
    18961 ctggttaata tcggatcgcc aatcaatcct gcctgctcta acgccagctc gctggcacgg
    19021 gtcgacattt gcgacacgcg gcccatcgca cggatacgct tgcgggtgta gtgctccggt
    19081 agcgtgaaat catcaacagg cgcgccaagc agggtgtgca gaccatcgta gacctgccac
    19141 tccggcattt tgcgcactgc attctcatac gccagcagac gcgcggaaac gtcctgccag
    19201 ttttcaccaa aggcagtcac gccgcccatt cctgtaatca ctacgcgacg tgtcataaca
    19261 tccctccgtt gatggaaata acctggcggg tgacgtaacc cgcaatatca gacattaaat
    19321 agctggcaag cccggcaact tcttccgcct ggcccatgcg tttcatgggg atcatcgaca
    19381 ttgcctcttt cagggcgctc tcttccattt cgatcatccc ggtatcaata agccccggcg
    19441 cgatgcagtt gacggtgatt ttgcgcttcg cgagttcgat agccagtgct tttgttgcgc
    19501 cgataattcc ggctttggca gcgctgtagt tcacctgtcc gcgattgccc atcacgccgg
    19561 aaaccgagga cagcgtgatg atgcgtccac cctggcgtgt gccgatcatc ggcataatgc
    19621 acggctgaat aacgttgtag aaactgtcga gattggtgtg gatcaccgca tcccagtcgt
    19681 catcgcttaa agccggaaaa gcggcatcgc gggcgatccc ggcgttacta accacgccgt
    19741 accaggcacc gttttgcgcg atttcgtgct ccagcacttc ccgacactgt tcgcggtttg
    19801 cgacgtcgaa actgagcaaa cgcccgttac ccccgttggc gacaatggca tttagcgttt
    19861 cctgagcgcc tgtggcgtca cggtgatagt gaacgccaat attaaagccg tctgccgcca
    19921 gttggcaggc gatggcgcga ccaatgcctt tgctggcacc agtaaccaga actgaacgac
    19981 tcatcaggac gctccttgtt gaaacagggt ggtaagttct tccgcggtcg gctggaaggt
    20041 gttaatgcgg cccgttgccg tgccgttgtt gatgctgcac tcaaaactgc cgaagcgatc
    20101 gtcttgcatc aggagtttga cggtaatggt cagcgtctgg cctgcgggca gaatgccagc
    20161 ggcgcaaagc aattcacgtg cgcccagtac catccctaac tcaatactgt ttttgccctg
    20221 ctggtggcga tgccagccag accagacgcc gacggtttgc gccatcaatt caagggcaaa
    20281 ccagccaggg agattgccgt ccgggtcgag aaacggtgcc agtacgccac tgggcgagac
    20341 cgttacacgg cacaccgcgc tgtcatcgct gacgctcacc acttcttcca gcagtaacat
    20401 gggcgcgtcg tggggcagat aagcgccggg ggataaatag tggctcacga aaccctcccg
    20461 agcagaatgc tggcgttatt gccgccaaag gcgaaggaat tggacaggat caccggacgt
    20521 gccagcggct gtggttgttc gataatgccg cacggtggca gcgtgggatc gcgcggcgac
    20581 aggctgaagt cctgagcggg cagcggtaaa tcacgctcta agatcaacat actgattgcc
    20641 gcttcggtaa tgcccgctgc gcccagcgtg tgtccggtca aatgtttggt ggagctgcac
    20701 ggtacgcgtt cgccaaacag ggcgtttacc accatcgatt caatctggtc gttgagttgt
    20761 gtggcggtgc cgtgcaggtt gatatagccc acctcatccg gcgtaagctg cgcatcagcc
    20821 agcgcctggt taatggcgcg aattgcgcct tcgccctgtg gatgcggggc cgaaatatga
    20881 taagcgtcgc tcgattcacc gacgcccagc aaggcgatgg gctgcggttc gcgggtcaac
    20941 agcatcagcc ccgcaccttc gccaatggtg atgcccgcgc ggtcacgacc aaacggctga
    21001 cataaggttg gcgagagcga ttcgaggctg tgaaaaccgt taatcggcat tcggctcagg
    21061 gtgtctgcac cacccacaat ggcggcatcg accagccccg cctcgataag cctgcgcccg
    21121 ctgatcatcg ctctggcact ggaggagcag gcggttgaaa gggtataagc tgggccgtcg
    21181 agcgccagcc agtggctgag aaaacgcgac ggatcgccga gctcttgttg tgcatagtgc
    21241 cacgtggtgc tcggctgccc gtgggtgcgc aggttaacgt gcgtgtcgcc ttcatgcagg
    21301 ccggaggtgc tggtgccaag cacaatcgcg atgcgctcgc ggccatattt cgcaatagcg
    21361 tcatcaacct gcggctggat ctgcgccagc gccgccagca ggatctgatt gttgcgcgag
    21421 cggtgcgcag cgaatttttc cgggataacc ggaagctcgc catccacgcc agccaggacc
    21481 gcctgcggat gcccctgcaa ccagcctgcg cgcgggcgca tgccgggagc gacaccgcga
    21541 gtcaggttgg ctgcgatatc gtcaaggtta tttcccaggg cgttgatcat gccgacggcg
    21601 gaaatgtaaa tcatatcagt cacctaaata ttgaatggtg atgtggtatt taaagacatg
    21661 ctgttcaatg ctgattggca cgcgttttcc ctggcgattc agataggtga tttccgtgac
    21721 cagtttgccg ctggcgttac gcagttcgcg tttgtcaccg ttgtcgcgaa gcgtccatcc
    21781 agcggggagt tgcggttgcc aggcgctaat cggccagtgg ctaagcatca cgtcagccag
    21841 cacctgactt gcgggcggca actgtgggac gacgatggat tgctcggcgc gtagcccttg
    21901 cgcatcgtag gtcaccagaa acagacgaat gccgaccgac gacagcccgg caagggtgat
    21961 tttctggtcg tcggcgttaa gcatcactag cagagactgg gttttgccgt tgaagctgcc
    22021 gctcaatagt tgctgggaat taacggcggg cgaaatccct ggtgccggca gcgtgatgcg
    22081 cgtgccaggt tgcagccacg cctgtgggcg accttcctgt tccggttgcg agtggctaca
    22141 gccggtgagg ataagcgtag cggtcagggc gaacgctcgc cagaatgtgg gtttgatcat
    22201 ttttttgttc tctttttatc gggcatagcc agcggcgaaa gcaggaaggc ggtgaaaata
    22261 ccgctcacca gcacaatgcc aaaactgctg atggcctggg tggcgctgaa taccagcatt
    22321 cccagcgcca gcaaggtggt gagcattgcc agcgtgatcg ccagtaggga agttaacggt
    22381 gtaccgcgcg gattactgaa aaacagcgtg taattgatgc cgatgccaag caccagcacc
    22441 agcgccagta gcgaaaagag attcaccgcc tgcccgctca tcgccagcac tgccagaccg
    22501 cagcccagcg aaagtaccga aggcaccagg ctgataagcc ctttgcgcca gccgaggcgg
    22561 gccactgcgc cgcaggcaat caccgccagc gccaccagca acaaaccggt taagacgtag
    22621 cggtaaagtg cgaacaactc atcaaaggtg cttttgcgat caacccaggc aatgccgcca
    22681 gggtaatcgc tggcgatttc ctgcaacaac gcgctacttt taaccccttc aaccggcacc
    22741 agtacgccgc tttcgccgtt tttcagcgtc agccacagca gacgccagcc ttcactggcg
    22801 gggcttgcca gccattcatc gaccttcact ggcatggcgt tgagatccgg attcactgcc
    22861 gccagcccgg cattttgcag tgcttttgtt actgtcgggg cagccgtttt cagcagatgt
    22921 aaatcttcct cctgccgcgc cagggagttc agcggaatgg tgcggtagtt gctgataagc
    22981 ccctcttttt tcgcattctc cagcgaggcg gtaaatttct ccagccgccg caatgtttgc
    23041 tgtggagagt cgccgtaaac tacaaaccat ttttgatcga cgctttgtcc ggtcagggcg
    23101 gtaatggctt tctcctgggc cagaatatgc tgcggtagcg cctgtaactg cgagatatcg
    23161 tcgtcgacgc gcagcattga catccccgcc agcgaaaaca gcgccagcgc gacgggcaga
    23221 cccagcgaca gttttttatt acgccgccac gcagccagcc agcgcagcat cagcgccatc
    23281 gccggaaccg gacgcaccgg caggccacgg cacagccacg gatgccagaa caggacggtc
    23341 agacaggagg cgctcaaccc gacggcggca aaaatcgcca tctggcgaat gccggggaag
    23401 ggggcgagca tcataatcag atacgccgcc acggtggtga gcagcgccag cagcagggca
    23461 ttgcgcactt tcgccaggct ttgccacggc gaaacgtcat tgccgtgcac catccgctcg
    23521 gtgaggtaat agagcgtgta gtcagcggaa atgccgataa cgctcatgct catcaccagc
    23581 gtcatcaggt gtaattcgcc gaaaatcaac aaggtggcga ccgttcccgc cagcgcgccg
    23641 atgccgatgg aaatcacgca aagcagcaat gggcgtaaag agcggaacac cgccacaatc
    23701 agtaatatca cccccagcag cgttgccacg cccaggttgg agatatcctg cttcgcctgt
    23761 tggctggcgt aatcgctgta aaacaccgtg ccgcgcgaga gcaactgcgc ctgcgggtaa
    23821 cgcgttttca gatccttttc cagcgtattc agggtcgtga tgagctggtg ggtttgctgc
    23881 atatcaaacg acgatcccgc cagttcgccg tgcagcagat accagtagtt gccctgggga
    23941 tcctgcgtca ccagccagcc gtccatcagc cgcaaacgct ggccgttttt cgccatcgcc
    24001 agctgcgagc cacgcattaa catcagcgga tcgttttgca gctccttacc gcttaccccg
    24061 gagaacgcag aataaagctg ggagaggatc cactgcgcct gcgcttcgcc gccgttttgc
    24121 aggcgcgcgc gggtgtcggg gtcaaccagg ccgttgcggt gctgccagaa aaacgctccc
    24181 cacgcctgct ggctggcggc atccattggc cctttaacgt cgccgagcgc agcggatttt
    24241 tgcagcagcg tcagccactc ctgagccacc ctgggattag cctctttacc ggggctgacc
    24301 agccacacca gttggcggtc cagacgctgc ataaagccgt cattcagcgc tggaggaata
    24361 tcgcccatcg cctgtttggg taacatagcc agcacgctac tgttcagtcg tgattgcggc
    24421 aacaggatca gcaacgccgc cgccatgacc aggcagacga tcccccataa cagcgccggg
    24481 cgtttactgg gcggcaaaac gttggcgttc gtcatcggtc agttgcgctg gcgtcagttg
    24541 atgttgggta agagcgatat cggtgcgatc gccctgttta tcattaagct gaatgctctc
    24601 cagataggtt tgcccggcga gatcgatggt gttgaaaatt ttatccagcg gcgtggtggt
    24661 cggcgtcagg cgcagtgtcc agcggccttc gcctttgtca gcaaattcga cgcggaaatt
    24721 ttgttccagc actttgcgat cggcctggaa cagcgcgcgc agcaggtggt taaactggaa
    24781 catctgcggg ttattttctg ccgtgatgat ttgcggcggc tgaccgttga tcacctgcac
    24841 catgcgttta tcgtccagca atagttgcat gggaaatggt gaggtttgat cccacaacag
    24901 cccctggtcg cgggcgatca acatctggcc ctgagatcgt agcggctgcg gcagatcttt
    24961 aatcgtccgg gtttgatcga aatgagcgcg gatcaccggc tgttcggtaa agcgttgctg
    25021 aagatcgtcc aggttcaggg cgctcacaaa cgggctaatc agcagcgcca gcagtggtaa
    25081 aaatttcatg gtttgactcc catgcgttca aacaaaatat cggggctgac aaagcacagt
    25141 tcgcggcttt gctcgtcgac cgcgacctga atggtgtatc cggtggtggc gcgtttgccc
    25201 gtttcggcat cgaaaatctg atagccaatc cgcagacggt tttcgaactc ttcgatatgt
    25261 gcacgcacgc ggatgcgttg ctcaaaggtg agggcgtggc gatatttcac ccgcgcatcg
    25321 accactggcc acaaataacc ggactctttc atctgccgat agccgtaatt gaactggttg
    25381 agcagcgcct cgcgggccac ttcaaagtag cgaaaatagt taccgtgcca ggccacgccc
    25441 atcatatcga cgtcgtgaaa gggaatggtc agctcgactt cagcggtaaa tcggggatcg
    25501 ttaagcaccc tttactcctt gtcctggact tccggcagtt gccagaaatc gaaaaaatta
    25561 aaccagtcga ggggcgactg gagcgcgtaa tgttccagac gcgcggcgta atgatcgata
    25621 gcgttttgca gcgcctgctg gcgttcgcca cgcggcaaca gcagcgggtc ggcaaaggtt
    25681 tcgcagtgaa tatgcagctt gccgtgctgg cgcagggcga aaatcagatt caccgggcag
    25741 cgcaaaatag acgcaagaat aaacggcccc tgtgggaaag gcgcaggctg gcccataaac
    25801 ggactccagc agacgcgcca gtcgccgccg cgttgcgggt tgacggcgat gcggtcaccg
    25861 acaatcgcca cccattcgcc gttatccagc ttctctttca gcaggatggc ggtttccggg
    25921 ccgatatctg ttaccgggag caggttgatg cctgcctgcg gggccatctc ctgcattatc
    25981 tgtttaaagc gttgggcgtt ttcgctaaac accagcgcgt taatggtttt gtagccctga
    26041 atttttgcca gcgcccggca cacttccaca tcgccaagat gcgaggccag cagcaatttg
    26101 ccgcgcggat cgctgacgtt aagtgccgct tctgcgcctg gtgcaaacag cacatcacgc
    26161 ccgagttgca gctcgccgcg ccagctggcg attttgtcga gcatggcgtt gccgaaacgt
    26221 agaaaatgct gatagctgtt gagttttgct gcctgtggtt gttgttggcg cacacgggcg
    26281 agccagtctt gcgacgcttt acgtgcacga gcagcggtga gccagtagac gcccaccacc
    26341 gggtaaagca gcgcggtaaa cgcagttctt cccagcagac gccagaccag cagcatcagg
    26401 cgcattcccc ataacccttt cacttcgctc tgccgcgccc agtggctgga agagtggcgc
    26461 atcagcagtg aaggaatacg cggcaacatg ccgaaaaaca gacgtgtgtg catcagcgag
    26521 atgcggacgt tatctttcag ggcatcaaaa tgcgaaagcc cgtccagcgg ataggtgacg
    26581 cgggtcggca cgaaataact ggtatttccc tgccagtaga ggcgcaccat cacttcggtg
    26641 tcaaaatcca tccgcttgcc gatggtagcg tattttgcca gttgcagcgt tggcgcaacc
    26701 ggataaacgc gaaagccgca catactgtct ttcagttgca gggagagcgt ttcgatccat
    26761 acccacacgt gggtgaccca gcgcccgtac agccgtgagc gggggatgga gtcatcgtaa
    26821 atcggctggc cggagatcag cgccgcaggc tgctgttcag cgagtgccag cagtttaggg
    26881 atgtcttcaa tcgcgtgctg accgtcagca tccacctgca ccgcatggct gaagcccgcg
    26941 tctgccgctg cctgtaagcc acgcatcact gccgcgcctt tgcctgcgtt ttctgccagg
    27001 cgaattaagg tcacgccagg ctgttcggca accagattat ccagttgctg ttgcgtggcg
    27061 gcatcgctgc cgtcatccac tacaatacag ggcagattaa atggcttaag acgcgccagc
    27121 acgcccggca tcatcgcgcc gtggttgtag caggggatca acacgcaggg agaaaagttt
    27181 accgacatag gcggatcttc ccgctactgg cggtgtggcg ggcgtcaccg tcgtgacgct
    27241 gatagctaaa gctcagaatc tggcgttctt cctgccagtt aagcgtgagc gtgacgcggt
    27301 tgtccggcag caacggggac tggaatttga tgttctgaat agagtgaaaa cgccagccag
    27361 gcgcgagcag agtgagcgcg tagctcatcg cccagtcgat ttgtgcaact cctgggagta
    27421 acggctgcac ggcaaaatgg ccgcgaaacc agaacagatc tgccctgaga tgcaaaacaa
    27481 tctcaaggtg attcgcttgt gcctgatggc gctcgatttc atggggcttc atgaaataac
    27541 tcctgtaatt gcgcatagac acgcttgttc atactgttta ctggcatttc atcaataacg
    27601 cgccagtaac gcggaatagc aactggctcc agcgtcgggc gcagtaatcg ccgccaggtc
    27661 atttcctggc tatgcccgcc gccgttctgc cattgcaggc gagcttcgtc atccagcacc
    27721 agcaaaacgc cgatgctctg acgcccgccg cgcgtaacgg gaactgccgc cgcctcgcga
    27781 atgccgtcca gcgccagcag acgttgttct acttcctgca gagaaatacg tttttcttca
    27841 atcttaacga tgcgcccacg acgccccatc agatggaact gaccgtcctg gctaaactgc
    27901 aagagatcat cgagtaacag gccgttatct tccgctatca gcggtgaaaa gagacgaaat
    27961 gcttcacctt cggcctggaa gtgcatacct ggaaagggca gccagcggcg ttgctcttgt
    28021 tcgcggtaac gccaggcaat gacgccggtt tccgtgctgc cgtagatttc atcaggccag
    28081 acgcgcagcc agctcgcggt gtgttttacg tcctgccacg gcagctcacc gccagcagag
    28141 agaagcacct gaacgggcgg cggagaaagc tgggtatcca gccgtttcaa aaatgccggg
    28201 ctgctgatga agatatagcg atgctggtga ctcaatgcgg caaactgttc gacataccag
    28261 agcatggcgg cgtgcagcgg taatcccagc gccattggca ggaaaacgcg aaaagtcagg
    28321 ccgtacagat gctgcggcaa tacggaaccg acgacacgac atccggcaag acgtgcgcct
    28381 aatcgttcag ccagcagttc tgcttcacgg tccagcaaat gaacaggttt aattacccgc
    28441 tttggctgcc ccgtagaacc ggaggtaaat agctcgatat aagcgtctgg cgctatagcg
    28501 gcaaaggtga atgattgtgt cgcgacttgt ggggaacttg ctaccaacag caatgaacct
    28561 tgccagttaa gatcgctgtc gctaagtaca ccatcgaaca gttcacgttg ttcattaagc
    28621 tggatgacac ggttatgccc cggaagcacc ggcgtttttc cggcgtgtag cgtggcaagc
    28681 agggcaacga taaacaaata actgttctca aagcacagcg cccagcgttc acccggctgt
    28741 tgctgcaagc ggcagataag ttgcgcgaca tcgtgacgca aatcgcccag tgtccacgag
    28801 ctttcgtcca gccacgcaat gggcgtgtca tcagggcgtg gcgcagtcag ccagcgtgca
    28861 agcggtaagg tctgcttcat gagcgatctc tttttatcac ctgacggcgc accagccatt
    28921 cacctgccat taacgttccc atcaaaaaat aagcaatcat gccgttccag gcggtccata
    28981 acgacatatc gccataaagc gcggtaaaga gggcaattcc gccgttaatg atgaaaaagc
    29041 cgcaccagat ttgcgtgaca cggcgggtat agcgcacgcc cttttccggg aggtctggct
    29101 cctgcaaacg tgccagccgc tcaactatcg gcattgtcga ccacagcgaa ccgccaaaaa
    29161 ccgccagcat gatcgcgtta accactaccg ggtaaaacag cagtagctgg tgcgttttca
    29221 acagaaagct ggcgacacac aatgcgatgc ccacgacagc gacgatttgt gtgacggctt
    29281 gtagcggccc cgcccggcga cgggtctggc gcaggcgcaa caatagcagc aacgctatta
    29341 gcggcaacaa ccagtgcagg ccgttatgcg ccaggccaaa ccaaatcaca aacggccagg
    29401 cgagcagcaa taatccagtc agcagcttaa tcatcggtag cgagcgaatg cccgacatca
    29461 gatcaggctt cttgtagcag gcgttctaca gcctctacga catcctgaac tgtgcgcact
    29521 gctttaaact cttccggctt gattttctta ccggttttct tttgcaggtg cacaatcatg
    29581 tcaacggcgt cgatgctgtc cagttccaga tcttcgtaca ggcgcgcttc aggtttgatg
    29641 tcctgcggat cgatttcaaa cagcttaacc agcagggctg agacttcctg atagacggtt
    29701 tgttgttctg tcatgatggc tttcctcagg cgcgttgcgc agcaatgaaa gaggccagag
    29761 tggcgacgga gaaaaagtgc tgacgcatct cttcgctttc ggctgagagc accacgccgt
    29821 actcattttt tactgccagt cccagttcca gggcatcgat ggaatccagt cctaaaccgt
    29881 cgccgaacag cggcgcatcg gtctcgatat catcaggagt caactcgtcc agattgaggg
    29941 tgctgatgat gaggttttta atttccagat aaagcgcttg cattatttat tcctgagaga
    30001 ggtaaacaac cttgttgtaa tagaagcaaa agatgccggt ttaactgcct tgctgccagc
    30061 gccggttctt gtgaagttgc atcgtaaaat ttattgattt caacccgacc atggacttca
    30121 accgtaaaaa acggccggtc aggtggaacg tcataccatt tactttgttt atccagcaga
    30181 tgctcgctac agccgatcgt cacgatgcgt aaatcgctgg cacagcgcac ggcaatattc
    30241 gccgccccgc gctgcagcgt cattttttca ccgggcttcg ttcgcgtgcc ttcggggaaa
    30301 atcaggatag tgtcaccctg tgccagtcgc tgttggcagc ggggtaacag ggtttcagct
    30361 tcgctgttga ttaaataatc tgccgcccgc acgacgccgc caagaaaagg attttttaac
    30421 agcgcgcttt tcaccaggca gtcagtttct ggcatgactg acgccagcag cacgtaatcg
    30481 atcagcgtgg gatgattggc tacgacgagg caaccgcgtt cctgacgtaa aatttcagcg
    30541 ccatcaatac gataatccag cacgccaagc cctttcgcga cagttaaaaa caggcgaaaa
    30601 ctggcggcaa tgctgcgacg cgccaaacga cggcggcggg aagtatccca caccaaaact
    30661 aacaatatgt taaaccacac gacagaaagc agcaggccgc cgagaccaaa cagggcaaaa
    30721 cataacccgg tcatgaccag tcgccaggtc cactccagac gtgacatcag cttactcatc
    30781 tgcggctcca gcgccattgc acgcgctcgc caggcagtga aaatgcgtct gcattttgta
    30841 aataatgctg taaaaacaac atgctttgcg gcagtgatgt ttcattgccc gcaatggctg
    30901 actgcgtttc acattgccag tcatcgccag attcaatcac cagcgcgacg gcgtacggcc
    30961 aggtcggcat ttctgatggc agttgtgggt gataaaactc gggcagaaaa ccgtcaaaat
    31021 cgaccatcaa cacgcgctgg tatcccgctt gtaacaggct aagcacttca cacaaacctt
    31081 gctggaaagt gtcgcgacca gcagaaagcg atgacgaaac tatcggctgt ttggccgcaa
    31141 tggtcaggtt acccacggaa gagttgtgta cggagagcgc gaagtcggtc ggagaaagcg
    31201 cctgttcggt tgccagtgca tgaacaatgc ggtaattgcg ctccagttcg ccatgacgac
    31261 tggtatacaa aacggcgtct ggctgatggc ggcgtagcat tgccagaccg cattcaacag
    31321 ccagtttgct gcctgaacta aggcggcggg cggtcatcat tggcagttca cttaattttg
    31381 ccaacggcgc ggcagggtca atcgcccacg gctggcgcga ccatgcttgc cattgctgcg
    31441 cttcgctaag tccaggcgcc agtgcctgcc agtcggtaat atttaatgta aatttcatca
    31501 acgcgcgccc gcggtaaacc aaaattatta agtgacgatt atttcttttg gcatatcaaa
    31561 agtgtatgcc caacgcctaa attatcgtgg cgctctgcta cctggaatcc tgctttattc
    31621 agatagtcgt aaaatttctc tgcactatag aaacggctgt taccgtttgc catgcaggta
    31681 aaataaagtg aggacgcgtt caggctaaat gatgctgctt caaaccgctg cgcatcccag
    31741 aacaactcca tgatgcaaac ttttgcgcca ggcttcatta cgctggcgat tttgctgagt
    31801 atgctaatta tttgttcggg tgaaaaacag tctaaaaatt ggctcatcca ccagatatca
    31861 gcctcgcccg gtaatggggc atcgcttagc atatccaccg catggaaatc tatgcgatca
    31921 gaaaaacctg cattcgcgat attttctttc gctagtacga tttgttgtgg cagatctaat
    31981 aacgtcacgg cgatgttttc attgtatttg cagcaacgca aagcccactt acccgtattg
    32041 ccgcctacat cgtacaatgt tgtggggttg ttggcgaata cataaggcag tgcggcgtta
    32101 aatgcgccat ctgaatagta atgatcaaac gcaaaccagc tttcacgagc ggcgtctggt
    32161 aattgtgaaa gtgccggata tatcgtcgac cagtcgccaa atacttttaa cccggacggt
    32221 tttccttcat tgagtgaatc ggcgaggaag aagagcccct ggtaacagac atcctgggtg
    32281 aaatccatat tcacgcgagt catagtgtca tgcaggaggt aatgaccgat tttcgccagg
    32341 taatacattc cctctttaca ggtaataatt cgcccgctta agcccatatc taacagaacg
    32401 ctggccgcat attcgttgat atggctatgt tcggtgatat cagccagcgt tgcgccatgt
    32461 ttgccttgcc gatccagata tgaaagtaca cccgcattac gtaggcagag tgctgtttga
    32521 aaaagcatag gggcaaaggc aatgcgttga gcttcggtaa tcgcatccag tgcgctaaga
    32581 gaatcctgtt cgtacatgtt gggcacaacc tgttaagagg gaattaaatt attatcggac
    32641 cgcagcggcc cgactttagg aagaattata aatctgcgcc cgctgacaaa ttcagttgaa
    32701 tatctttgtc gagattgcgg acccagcgat tgtagttttt atgaatttgt ccgcctgacg
    32761 cttgtaggtt ctggctattt tcataattga tatcgtagct ggatgccgtg tagttaatgc
    32821 gaatttcggc tgaatgatcg cgtgactgtt gacgtgcttt aattacgcct tgtccggtaa
    32881 tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca
    32941 gctccaccga ttttga
//
LOCUS       DAYPHN010000036        31924 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000036 DAYPHN010000000
VERSION     DAYPHN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.036"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(116..1408)
                     /locus_tag="ACG0BW_001672"
     CDS             complement(116..1408)
                     /locus_tag="ACG0BW_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFK7258646.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(1411..2343)
                     /gene="glsA"
                     /locus_tag="ACG0BW_001673"
     CDS             complement(1411..2343)
                     /gene="glsA"
                     /locus_tag="ACG0BW_001673"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFK7258647.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            2606..5110
                     /gene="copA"
                     /locus_tag="ACG0BW_001674"
     CDS             2606..5110
                     /gene="copA"
                     /locus_tag="ACG0BW_001674"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFK7258648.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHAVLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKHLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            5282..6076
                     /locus_tag="ACG0BW_001675"
     CDS             5282..6076
                     /locus_tag="ACG0BW_001675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFK7258649.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            6280..6759
                     /gene="ybaK"
                     /locus_tag="ACG0BW_001676"
     CDS             6280..6759
                     /gene="ybaK"
                     /locus_tag="ACG0BW_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFK7258650.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(6796..8448)
                     /gene="ushA"
                     /locus_tag="ACG0BW_001677"
     CDS             complement(6796..8448)
                     /gene="ushA"
                     /locus_tag="ACG0BW_001677"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFK7258651.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLDVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKINGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            8666..9886
                     /gene="fsr"
                     /locus_tag="ACG0BW_001678"
     CDS             8666..9886
                     /gene="fsr"
                     /locus_tag="ACG0BW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFK7258652.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            10124..11800
                     /gene="ybaL"
                     /locus_tag="ACG0BW_001679"
     CDS             10124..11800
                     /gene="ybaL"
                     /locus_tag="ACG0BW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFK7258653.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(11930..13234)
                     /gene="gsk"
                     /locus_tag="ACG0BW_001680"
     CDS             complement(11930..13234)
                     /gene="gsk"
                     /locus_tag="ACG0BW_001680"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFK7258654.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            13386..14345
                     /gene="aes"
                     /locus_tag="ACG0BW_001681"
     CDS             13386..14345
                     /gene="aes"
                     /locus_tag="ACG0BW_001681"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFK7258655.1"
                     /translation="MKPEKKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(14342..15304)
                     /gene="hemH"
                     /locus_tag="ACG0BW_001682"
     CDS             complement(14342..15304)
                     /gene="hemH"
                     /locus_tag="ACG0BW_001682"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFK7258656.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(15436..16080)
                     /gene="adk"
                     /locus_tag="ACG0BW_001683"
     CDS             complement(15436..16080)
                     /gene="adk"
                     /locus_tag="ACG0BW_001683"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFK7258657.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(16261..18135)
                     /gene="htpG"
                     /locus_tag="ACG0BW_001684"
     CDS             complement(16261..18135)
                     /gene="htpG"
                     /locus_tag="ACG0BW_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFK7258658.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(18245..18850)
                     /gene="recR"
                     /locus_tag="ACG0BW_001685"
     CDS             complement(18245..18850)
                     /gene="recR"
                     /locus_tag="ACG0BW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFK7258659.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYGVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(18850..19179)
                     /locus_tag="ACG0BW_001686"
     CDS             complement(18850..19179)
                     /locus_tag="ACG0BW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFK7258660.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(19232..21163)
                     /gene="dnaX"
                     /locus_tag="ACG0BW_001687"
     CDS             complement(19232..21163)
                     /gene="dnaX"
                     /locus_tag="ACG0BW_001687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFK7258661.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVIMPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(19835..19899)
                     /gene="dnaX"
                     /locus_tag="ACG0BW_001687"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(21292..21843)
                     /gene="apt"
                     /locus_tag="ACG0BW_001688"
     CDS             complement(21292..21843)
                     /gene="apt"
                     /locus_tag="ACG0BW_001688"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFK7258662.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(21996..22373)
                     /locus_tag="ACG0BW_001689"
     CDS             complement(21996..22373)
                     /locus_tag="ACG0BW_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFK7258663.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            22443..22970
                     /gene="priC"
                     /locus_tag="ACG0BW_001690"
     CDS             22443..22970
                     /gene="priC"
                     /locus_tag="ACG0BW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFK7258664.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            22984..23148
                     /gene="rsmS"
                     /locus_tag="ACG0BW_001691"
     CDS             22984..23148
                     /gene="rsmS"
                     /locus_tag="ACG0BW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFK7258665.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(23187..26549)
                     /gene="mscK"
                     /locus_tag="ACG0BW_001692"
     CDS             complement(23187..26549)
                     /gene="mscK"
                     /locus_tag="ACG0BW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFK7258666.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26677..27324)
                     /gene="acrR"
                     /locus_tag="ACG0BW_001693"
     CDS             complement(26677..27324)
                     /gene="acrR"
                     /locus_tag="ACG0BW_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFK7258667.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPAITE"
     gene            27466..28659
                     /gene="acrA"
                     /locus_tag="ACG0BW_001694"
     CDS             27466..28659
                     /gene="acrA"
                     /locus_tag="ACG0BW_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFK7258668.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            28682..31831
                     /gene="acrB"
                     /locus_tag="ACG0BW_001695"
     CDS             28682..31831
                     /gene="acrB"
                     /locus_tag="ACG0BW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFK7258669.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         7339 a         8421 c         8469 g         7695 t
ORIGIN      
        1 acagatttat cgcgtatata tcattttttt gtgacttatt tattaaagga ataagaaagg
       61 aaatttgaat taaagacaaa ggcgcagtac aggaagtact gcgcacagag aggtgttata
      121 cggttttatt gcgcttcatg accattgcca caataaggct gagtaagatt gcgccaataa
      181 tccatatcaa cgcacggctg ccctgggacc acaggctgta gataaagcca acgatcacca
      241 gcaacataac caacgtacca acaataagaa tcggcaacga ggcgtgaata tcatgacgca
      301 ggcggatcgc caccacaaac accgccagat agcagatcaa aaaggtggcg ctggcaacgc
      361 tggcgagtga gcctaaattc agcgccgccg tcatcagcat aatcaacacc acgacaatga
      421 tgttgcccca ggtactctgc cgccacaggg atttattcat tagcttcggc agttcgcgtt
      481 cgctgcccat gttgtccatg atgttaaaca cggcgaacag gttcgcgttg atggctgaag
      541 ccgtcgccag taaagcgccg atgacgacga tcacataacc cacatgcccg agcagcggag
      601 aagctgcctg cgctacggca gtatcggcat atttttctaa ctctaatgcc gatacatcgc
      661 taagcaaaac cagcgccagc gagatataaa gcaacgtggt aacgccaatc gccaccagaa
      721 acgcccgtgg cataatgacc tgcggatctt tcactttatc cgccgcgttc gccatcatgc
      781 caaagcccgc ataggcaagg aaggttatcc cgatacagga gaagaacgca ccggagctgg
      841 ggggcgcaga gacggaaata tgcgccggtt gcagcgacca gacacctgca atgatcagca
      901 ataacaggat catcatttta atgccgacga ggatcacttc cagccgccct accgcatggt
      961 tgcttaagga gttgaaaagc gtcatcaccg caatgatccc caacgcgtag agcaaaataa
     1021 ggtgctcctc ctggctgcct tcatgcaaaa actgcacggc ataagcgcca aaagcacggg
     1081 cgaccatggc gatgctcacc gccagcgtca acaggtacag taacgagagc gccagcgaaa
     1141 agacaccgtt gcctaatccg cgacgaaaga agtcgataat gccgccattg ctgggatagc
     1201 tcgcccccag acgcgcatag gcataaccgg aaaacatcgc cacaataccg ccaaaagcaa
     1261 aggcgaccca ggtcgaggct tccattagca acgcagcctg ccccagcagc gcgaagatcc
     1321 ccgcccccac cattgcccca atgccgatgg aaacgacatt ccatagaccg aggggtttgt
     1381 taccgttatt accttccgtg ttcatcatga tcagccctta aacacgttat agccgagttg
     1441 cttagcgacc gatgccacca ttttttgacc gcgaacactg ttgccttctt cgtccagcgg
     1501 tggtgagaac gcggcaattc ccatcacgcc agggacgacc gccagaatac cgccacctac
     1561 accgcttttg cccggtaaac caacgcgata cgcccagtca ccggagcgac catacagccc
     1621 ttccatcatc atttcagcca gaatgtacgg cacgttgtcg gcctgaagaa cgcgtttatg
     1681 cgtcagcgga ttcacaccac ccgccgccag cgtcgcgcca agcgttgcca gttcaacagt
     1741 attgatgagc gtggagcact gacgggtgta cacgtcacag gcttccattg catcacaata
     1801 gagatatccg gcggagtaca gcagccaggc aatggcccgg ttatggaagt tggttgtttg
     1861 ttccgactgg tttacttcgt cagagagcgc tacctgctca ccagccagtt gttgttggat
     1921 atgtaaaatt cgctgccagc gttgttcggc attttcagcg ttaatcaggc tggtggtggc
     1981 aatagcgcca gcatttacca gtggcgacag cggtttgccg ccatgcaact ctaaggcgat
     2041 aactgagtta aagggcaatc cggtcgggtc agcgccaact ttgtcctgta ccgcctgcgg
     2101 gccgacatct tctaacgcaa gggctaacgt acagactttc gagatggatt ccagcgcaaa
     2161 gcggtaatca ctatcacccg cgcgatagat gttgccatcg caggtcacaa tagccactgc
     2221 tgccagttga ccaggtacat tcgccagaaa gggaatgtag tcggcatttt gtccgccgtt
     2281 aagtgagtga aattgggtgt aagcctgatc cactgcctgc tgtaatttgt ttgcatctaa
     2341 catcttttgt taactccttt ttatagatgc gggaggtaat tcctcacccc ggtgccgatt
     2401 ttcaggcatc ctgatttaac ttagcacccg caacttaact acaggaaaac aaagagataa
     2461 atgtctaatc ctgatgcaaa tcgagccgat tttttaatct ttacggactt ttacccgcct
     2521 ggtttattaa tttcttgacc ttccccttgc tggaaggttt aacctttatc acagccagtc
     2581 aaaactgtct taaaaggagt gttttatgtc acaaactatc gacctgaccc tggacggcct
     2641 gtcctgcggt cactgcgtta aacgcgtgaa agaaagtctc gaacagcgcc cggatgttga
     2701 gcaggcggat gtgtctgtca ctgaagcgca cgttaccggg actgccagtg cagaacagct
     2761 gatcgaaacc atcaaacaag cgggttatga cgcatctgta agccacccaa aggctaaacc
     2821 gctggcggag tcatcaatcc cgtcggaagc actgacagcg gtttctgagg cgcttccggc
     2881 agcgaccgcc gatgacgatg acagccagca gttgctgctg agcggcatga gctgcgccag
     2941 ctgtgtcacc cgcgtacaaa atgcgctgca aagcgtaccg ggcgtcactc aggcacgggt
     3001 aaacctggcg gagcgtactg cgctggtgat gggcagtgcc tccccacaag atttagtgca
     3061 ggcggtggaa aaagcgggct acggcgcgga agcgattgaa gatgacgcta aacgccgcga
     3121 gcgccagcaa gaaaccgccg tcgctacgat gaagcgcttc cgctggcagg cgattgtcgc
     3181 actggcggtg ggtatcccgg tgatggtctg ggggatgatc ggcgataaca tgatggtcac
     3241 cgctgacaac cgcagcctgt ggttggttat cggcctgata accctggcgg taatggtttt
     3301 tgccggcggc catttttacc gcagcgcatg gaaaagcctg ctaaacggtg cggcaacgat
     3361 ggatacgctg gtagcgctgg gtactggcgt agcgtggctc tattcgatga gcgtcaacct
     3421 gtggccgcag tggttcccga tggaagcgcg acatctttat tacgaagcca gcgcgatgat
     3481 tatcggtctg atcaatctcg gccatatgct ggaagcgcgc gcgcgtcagc gttcttctaa
     3541 ggcgctggag aaattactcg atttaacccc accgacggcg cgcctggtca ccgacgaagg
     3601 tgaaaaaaac gtgccgctgg cagatgttca gccaggtatg ttactgcgcc tgacgaccgg
     3661 cgaccgtgtg ccggtagatg gtgagattac ccagggcgaa gcgtggctgg atgaagcgat
     3721 gctgacgggc gaacctatcc cgcagcaaaa aggcgaaggc gatagcgtcc atgccgggac
     3781 agtggtacag gacggcagtg tgctgtttcg tgccagtgcg gttggcagcc ataccacgct
     3841 gtcgcggatt attcgtatgg tgcgccaggc ccagagcagc aagcctgaaa tcggccaact
     3901 ggcggataaa atctccgctg tgtttgtgcc ggtggtggta gtcattgcgc tggtcagtgc
     3961 ggcaatctgg tatttctttg gcccggcacc gcaaattgtt tataccctgg tgattgccac
     4021 cacggtgctg attatcgcct gtccgtgtgc gctgggactg gcgacgccga tgtcgattat
     4081 ttcgggcgtc gggcgggcgg ctgagtttgg tgtgctggtg cgggacgccg acgcgctgca
     4141 acgcgccagt acgctcgata ccgtagtgtt cgataaaacc gggacgctga ccgaagggaa
     4201 gccgcaggtt gtcgcagtga aaacatttgc tgatgttgac gaagcgcagg cgctgcgtct
     4261 ggcggcggcg ctggagcaag gttccagcca tccgctggca cgagcgatcc tcgataaagc
     4321 aggtgatatg cagctgccgc aggtcaacgg tttccgcaca ttgcgcgggc tgggcgtgag
     4381 cggtgaagct gaaggtcatg cggtattgct gggcaatcag gcgctgttaa atgaccagca
     4441 agttgatact aaagcgattg aggcagatat ttcagctcag gcatcgcaag gggcaacgcc
     4501 tgtgctgctg gcggttgacg ggaaagcggt agccctgctg gctgtacgcg atccattgcg
     4561 tagtgatagc gtggcggcgc tgcaacgcct gcataaagcg ggatatcgtc tggtgatgtt
     4621 gaccggggat aacccaacca ccgccaatgc gatcgccaaa gaagcaggga ttgatgaggt
     4681 gatcgccggg gtgctgccag acggtaaagc cgaagcgatc aaacatctgc aaagtgaagg
     4741 acgtcaggtg gcaatggtgg gcgacggcat taacgacgcg ccagcgctgg cccaggcgga
     4801 tgtcggcatt gcgatggggg gcggcagtga tgttgccatt gaaaccgcgg cgattaccct
     4861 gatgcgccat agcctgatgg gcgttgcgga tgcgctcgct atttcccgcg caacgctgcg
     4921 caatatgaag cagaacctgc tcggcgcgtt tatctacaac agtatcggta ttccggtcgc
     4981 cgccggtatt ttgtggccgt tcaccggtac gctgcttaac ccggtggtcg ccggagcggc
     5041 aatggcgctc tcatcgatta ccgtagtgag taacgccaac cggttgctgc ggtttaaacc
     5101 gaaggaataa gtacatcatt gcggatgcgc tgctttaggg tggcgcatcc gttataggga
     5161 aattattcta cgcgaccttt tttgcacgca tacaaacaca tcaaacaagt ccccaactcc
     5221 atttgcgatt tgcgccatcc ctccccgcgc gctagcatga tatttcacaa agggaggtcg
     5281 tatggatctg ttgtaccggg taaaaacgct ttgggccgcg ctgcgcggta atcattacac
     5341 ctggcctgct atcgatatct ctcttcccgg caatcgccat tttcatctga ttggcagtat
     5401 tcatatgggc agccacgata tggctcccct gcccacccgt ttgctcaaaa agctcaaaaa
     5461 cgccgatgcg ctgatcgtcg aggcggatgt ttccaccagc gatacgcctt ttgctaattt
     5521 gcctgcctgc gaggcgctgg aagagcgcat tagcgaggag caactccaaa atctgcagca
     5581 cattagccag gagatgggca tttctccctc actcttttct acccaaccgc tgtggcaaat
     5641 cgcgatggtt cttcaggcga cacaggcaca aaaactgggg ctgcgggcag aatacggtat
     5701 cgattaccag ctattgcagg cggcgaagca acaacataaa cccgtgattg aactggaagg
     5761 ggctgaaaac cagattgcca tgttgctcca gctccctgac aacggactgg cactgctgga
     5821 cgatacgctg acccactggc acaccaatgc acggttgctg caacaaatga tgagctggtg
     5881 gctgaatgcg ccgccgcaaa ataatgaaat aacgctgcct aatacgttca gtcagtcgct
     5941 gtacgatgtg ctgatgcatc agcgaaatct cgcctggcgg gacaaattat gcgccatgcc
     6001 gccggggcgc tatgtggtcg cagtcggtgc actacacctg tatggagaag ggaatttgcc
     6061 gcagatgttg cgttaaaaaa atggccaata tcgctattgg cccgtcaaag aggaatttca
     6121 tatttttatt attatgccgt cactttaagc gacggtgtaa ctcgattgtc gctcaatctc
     6181 accatcctgc caatactctt aggcaagatg actctttttt ttggaccgcc atcaggcgta
     6241 tgcttgcttc gtttttttgt ttaaggcagg aaaattacca tgacacccgc agttaaatta
     6301 ctcgaaaaaa acaagatttc gtttcaaatc catacctacg agcacgatcc ggctgaaacc
     6361 aattttggcg atgaagtcgt caaaaaatta ggtttgaatc cggatcaggt ctacaaaacg
     6421 ctgctggtcg cagtgaacgg tgatatgaaa caccttgccg tggccgttac gccggtcgca
     6481 ggtcaactgg atcttaaaaa agtagcaaaa gcgctgggtg ccaagaaagt tgagatggcc
     6541 gatccgatgg ttgcgcagcg ttcgacggga tatctggttg gggggattag cccactgggg
     6601 cagaaaaaac gtctgccaac gattatcgac gcccccgcac aagaatttgc cactatttat
     6661 gtttccggcg gcaagcgcgg gctggatatc gaactggcgg caggcgatct ggcgaagatc
     6721 ctcgatgcca aatttgctga tatcgcccgc cgcgattaag cattgtgccg gatgcgaaca
     6781 tccggcactt tcggattact gccagctcac ctcacccttc ggttcataaa cactcacatc
     6841 cagcggcgag cttttctgga tatacgcttt cagcacttcg gcatcaataa agccggtatt
     6901 cacatagccc ggtttgttat caaggcgcgg atagccatca ccgccggtgg cattgaagtt
     6961 taatgtcgcc atgcggtaag ttttcgccgg atcgaccggt tcgccgttga ttttcaggtc
     7021 gttgagtttg ccgtctttcg ccacaaagct aacgttggca aattgcgggt aggcacctga
     7081 atctggcttc atttgtgcga cggcggtcag gtaatcaatc acctctttcc cggtcatgtc
     7141 ggcatacacc accacattgc cgaatggctg cactttcagc acgtttttat agctgatatc
     7201 gcctgcttcg atagaatcac gaattccgcc tccgctcatc accgcaaagt cagcaccagt
     7261 gcgatccatt tgggctgcca gaattaaccg ccccatattg gtctgtacaa aacgcacttt
     7321 gtcacgatcg ccttccagac gaccattggt ttcgcctatt ttcacgtcca gctgcgcttt
     7381 gcctttgttc tggaacggcg ataacagcga gatcatttgc tggttttcag cgatttcagg
     7441 ggtgtacagc acgcgctcgc ttttcccgtc ttcccaggtc actttcttct tcaggttcac
     7501 cggaatcagc tggtagttaa ccattttcat ttcgccatta cgaaactcaa aatcagcccg
     7561 tcccacgtat ttgccccact catgcgcctg cacaatccag atgccgtttt gttgatccgg
     7621 tttgcatggc gtacccggca cgtaatcgac ctgttttttg ttttctgccg ccatgcagac
     7681 cggatcttgc gagtgaccac cgacgatcat cgccagcgat ccggcaggca gcgcgcgcgc
     7741 catctccaca tcgcccggtg cgttagagcc gtgctcacca ttgtcgtaat gccccatatg
     7801 ggtcgccgcg ataataatgt ctggcttttc tgtctgttgc agctcctgaa tcaccagctt
     7861 cgcttcatcg gcgggcttac ggaactcaat atcggtgaag ttttctgggt tgcctaactt
     7921 cgctgtgtca tccgtcgtca ggccaataac ggcaattttc agatcctgac gcttaaacag
     7981 cgcccatggt ttaaacaggc gctcgccggt acttttctgg tagatattgg cagaaagtaa
     8041 cgggaacttt gcccactttt cctgctggcg taatacggtg agcgggttat caaattcatg
     8101 attaccgatt gccatcgcat cgtaacccac cagattcatg ccgcgaaaat caggttcggc
     8161 atcctgtaag tcagactcgg gcacgccagt gttaatgtcg ccaccggaaa gtagcagcac
     8221 gctaccgcct tcagccgcaa cctctttgcg gataccatcc accagcgttt tttgcgccgc
     8281 cagaccatat tcgccatatt cattgcgcca aaaatgccca tgatgatcat tggtatgcag
     8341 aactgtaatt ttgtaggttt tatcctgctc atacgccaga gcagcttcac tcgccagtgt
     8401 aaatgtggtt aacagtgcta acgccacgcc ccgctgcaat aatttcatac ttctctccct
     8461 gacctgattt caacgtgttg aaaaatagta tgacgcgaca tcatagacaa gtctgcgtcc
     8521 ggtgaagata aatctgcatc cagattagaa attctgtatt cagcgttgag acttccttca
     8581 ggcgacgcat acaggtatgt tagtcgggta ataatcacaa ataagatcat tctttacatc
     8641 gcaaccctga aaaagtgact ttcctatggc aatgagtgaa caaacccagc ctgtggcggg
     8701 cgcggctgcg tcaacgacca aggcccgaac atcgtttggt attttaggtg ctatcagcct
     8761 ctcacatctg ctgaacgaca tgatccaatc gctgattctg gcgatttatc cgctgcttca
     8821 gtcagagttt tctctgacat ttatgcagat tggcatgata accctcacct tccagctcgc
     8881 ctcttcgcta ctgcaaccgg tggtcggtta ctggaccgat aaatatccga tgccgtggtc
     8941 gttgccaatt ggcatgtgct ttaccttaag tggtctggtg ctgctggcgc tagcgggcag
     9001 ttttggcgca gttctgctgg cggcggcgct ggtcggtacc ggttcatcgg tctttcatcc
     9061 ggaatcttct cgcgtagccc gtatggcttc cggcgggcgg catggcctgg cgcaatctat
     9121 ctttcaggtc ggcggcaact ttggcagttc cctggggccc ttgctggcag cggtaattat
     9181 cgcgccttat ggcaaaggca acgtagcctg gtttgtgctt gcggcactgc tggcgatcgt
     9241 ggtgttggcg caaatcagcc gttggtactc ggcacagcac cgaatgaata aaggaaaacc
     9301 aaaagcgacg attatcaatc cactgccgcg caacaaagtg gtactggcgg tcagcattct
     9361 gttaatcctc attttctcga aatatttcta tatggcgagc atcagcagct attacacctt
     9421 ttatctgatg caaaaattcg gattatctat ccagaatgcc cagcttcatc tgtttgcctt
     9481 cctgtttgcc gttgcggctg gtacggtgat cggcgggcct gtaggggata aaattggacg
     9541 gaaatatgtg atttggggct ctatcctcgg cgttgcgccg tttacgctga ttttacccta
     9601 cgccagcctg cactggacgg gggttttaac agtgattatt ggatttatcc tcgcttcggc
     9661 attctctgcc attctggtct acgctcagga gctgcttccg ggacgtatcg gtatggtttc
     9721 tggactcttt ttcggttttg ctttcggcat gggaggtctg ggagcggcag ttctggggct
     9781 tatcgccgac cacaccagca tcgagttagt ctataaaatc tgtgctttcc tgccactatt
     9841 ggggatgttg accatattcc tgcctgataa ccggcataaa gactgatttc attgcagcca
     9901 aaggcaaact ttggctgcat cgttttacag tcgccataag cctttcctct gttaaatcgc
     9961 cttctgtttt ccgatccgtg ctttttttgc agctaattcc atttttctgc aaaattcaac
    10021 aattattcat aaacatcatc aatgaaattg tcataaacta ttacctgtaa ttttggtttt
    10081 cccggccaaa aatggacaat aaccaccagg aaaaggagac ggaatgcatc acgccacccc
    10141 gcttatcacc accattgttg gcggccttgt gctcgccttt atcctcggca tgctggccaa
    10201 taaactacgt atttctcctc tggtgggtta tctgttagcg ggtgtgctgg caggaccatt
    10261 cactccgggc tttgttgccg ataccaagct tgcgccggaa ctggctgaac tgggcgtcat
    10321 tctgttgatg tttggcgtgg gtctgcactt ttcgctcaag gatttgatgg cggtaaaggc
    10381 catcgccatt cccggtgcga tcgcccagat agccgtggcg acgctgctgg gtatggcgct
    10441 ctccgccgtg ctgggctggt cgttaatgac cggtatcgtg ttcggcttat gcctttccac
    10501 cgccagtacc gtggtgttac tgcgcgcact tgaagaacgg caattaatag acagtcagcg
    10561 tgggcaaatc gccatcggtt ggttgattgt ggaagacctg gtaatggttc tgacgctggt
    10621 gttgctgccc gcagtggcag gaatgatgga acaaggcgat gtgggctttg ccactcttgc
    10681 tgtcgatatg gggatcacca tcggcaaggt gatcgcattt atcgccatta tgatgctggt
    10741 aggtcgccgt ctggtgccgt ggattatggc acgcagcgca gcaaccggtt cccgtgagct
    10801 gtttaccctg tcggtgctgg cgctggcgtt agggattgcc tttggtgcgg tggagctgtt
    10861 tgatgtctcc tttgcactcg gtgcgttctt tgccgggatg gtactgaacg agtctgaact
    10921 gagtcaccgt gccgcccacg atacgctgcc attgcgcgac gcgtttgcgg tgctgttttt
    10981 tgtctccgtc gggatgttgt ttgatccgtt aattctgatt caacaaccgc tggcagtgct
    11041 ggcgacgctg gcgattattc tgtttggtaa gtcgttagcc gcgtttttcc tggtgcgact
    11101 gtttggtcac tcccaacgta cggcattaac tatcgccgcc agcctggcgc aaattggtga
    11161 gttcgcgttt atcctggcgg gactgggcat ggcgctggat ctgttgccgc aagccgggca
    11221 gaacctggta ctggcagggg cgattttgtc gattatgctc aatccggtcc tgtttgcgct
    11281 gctggagaaa tatctggcga agaccgaaac gctggaagag cagacgctgg aagaggctat
    11341 cgaagaagag aagcagatcc cagtggatat ttgcaaccat gcgctactgg taggttacgg
    11401 tcgtgtaggc agcctgctgg gggagaaatt gctcgcctct gatattccgc tggtggtgat
    11461 tgagacgtca cgaacccgtg ttgatgaact gcgagagcgc ggggtccgcg cagtattggg
    11521 caatgcggcg aacgaagaaa ttatgcaact agcgcatctg gaatgtgcaa aatggctgat
    11581 cctgacgatt cccaacggtt atgaagcggg tgagattgtg gcatctgccc gcgcgaaaaa
    11641 tccggatatt gagattattg cccgcgccca ttatgacgat gaagtggcgt atatcaccga
    11701 acgtggtgca aatcaggtag tgatgggtga gcgggaaatc gcccgtacta tgctggaact
    11761 gcttgaaacg ccaccggcgg gtgaggtggt gacggggtaa acggtggttg ccggataata
    11821 ggcgcggata tgtaggcctg ataagacgcg ccagcgtcgc atcaggcatt gagagcaaaa
    11881 tgccagatgc ggcgtgaacg ccttatctgg cctacgaacc gacgataact taacgatccc
    11941 agtaagactc ttccaggctg tcttcacgct ccggcaagcc gcgcgttaaa cgaggtgaat
    12001 gctggttcag tacctgatag ctcacacggt tagcatattt acacacctgc gctaacgatg
    12061 aataagttaa ccaggtgaat ttatgtttgc tggagtttgg tacgttgcta cggtggtagc
    12121 tgttggcggt aatgtcatgc agcaacgctg ccagtgcgcc atcccccgct ccattagtgt
    12181 tcatgatttt ttccggaccg cccatatacg gcgcaatgtg cgaatagaca cgcagcggat
    12241 tctggcaatc cttgtggcgc atggcacggc taaactcata ctggttaaat tccgctatag
    12301 cgcccggcag cagcggatgc tgggttttac gtttcgcttc gtcttcagta aagcccgcca
    12361 tatacaagcc gattggcccg gcggtgcaca gcaccagatc aacccagtcc agcgccttgt
    12421 cagatgctaa caacggatcg ctttctccgg tcaacgcttc ggcttcatct tcgttcatcg
    12481 caaggataga aacgtggtct ttgaggaatt gctgccacca ctgcggattc tcggcaatga
    12541 caaacttggt gcccagcgtc agcaccaccg gtacgttata tttcttcgcg tactcaatgg
    12601 ctttcatggt tgcttccggc atgggttcac ccggcttgca acgcaccaga taagaagtga
    12661 gaaccagtgc cgaggctccg gcaatcacat cttccggaat actttcagcc cgcagctggt
    12721 tcatgtggcc agggctgata gcaaaggtac gttccccgga ctcgccaatc agcgtaaagc
    12781 aacgaccaat cgggccatcc acgccttgta gatagttaag atcggtacgg ctggaggtgt
    12841 tacacaggta acgataggca tagctgccaa tttcaatatt gctgcacatg acgcccagca
    12901 gcaccgaacg gtcgtccgcg agcaccgagt agttgtgcat ggtgttacca atagtgccac
    12961 ccgcaaattg atgggtaatc aggtttttct gttttagttc ctgataaagc gcttcggcta
    13021 cgtcatcctc aatcaccagt gaatgcccgg cgcttaatcc ataacgctca atgaattcgt
    13081 catccacttt cgcttcaata tcgaccagcg tttgatcgat acccactacc caggcggcgc
    13141 tggtttcgtt ttctggctgg aattgctgaa gcagcggatc gcgtgcattt acagggaagt
    13201 aatgtttgga tttacgttta ccgggaaatt tcatggttgt ttttacgggt ggttaactga
    13261 gcggcgaatg ttaacacaat ctgacgggag cgcgcgagac gcagccagtt tcaatatcag
    13321 caggcgggat cgcattttca tggcagtggt tccttacaat gacgtaattt gaaaggagtt
    13381 tttgtatgaa gccggaaaaa aaattacctg ttctggacct tatttctgct gaaatgaaga
    13441 ctgttgtgaa tactcttcag ccggatttac cgccctggcc cgcaacggga acgattgctg
    13501 agcaacgaca gtattacacg cttgagcgcc gattctggaa tgcgggcgct ccagaaatgg
    13561 caaccagagc ttacatggtt ccaacaaaat atgggcaggt ggaaacacgt ctcttttgtc
    13621 cgcagccaga tagcccggcg acgctatttt atttgcatgg aggcggtttt attctcggca
    13681 atctcgatac ccacgatcgc atcatgcgcc tgctggcaag ctacagccaa tgtacggtga
    13741 ttggtattga ttacaccctt tcacctgaag cgcgttttcc gcaagcgata gaggaaattg
    13801 tggctgcctg ttgttatttc caccagcagg cggaggatta tcaaatcaat atgtcccgca
    13861 ttggctttgc cggtgattcc gcaggtgcca tgctggcgct cgccagtgcg ttgtggttgc
    13921 gtgataaaca gatcgattgc ggtaaagttg cgggcgtttt gctgtggtat gggctttacg
    13981 gattacggga ttccgtgact cgtcgtctgt tgggcggtgt ctgggatggc ttaacgcaac
    14041 aggatttaca gatgtatgaa gaggcgtatt taagcaacga cgcggaccgc gagtcgccgt
    14101 attactgcct gtttaataat gatctcactc gcgaagttcc gccctgtttt attgccgggg
    14161 cggagttcga tccgctgctg gatgacagcc gtctgcttta ccagacgtta gcggcgcatc
    14221 agcagccctg tgagttcaaa ctctacccag gcacgctgca cgcctttttg cattattcac
    14281 ggatgatgaa aaccgccgac gaggctcttc gcgacggcgc tcagttcttt accgctcagc
    14341 tttagcgata cgcggcaaca agattagcca tcatctcgat atgttccggc gtggcattaa
    14401 gcgccggaat atattcatat tttttcccgc ccgcaccgag gaagacctca cggttttgct
    14461 cggcaatctc ttccagcgtc tccagacaat ccgcagcaaa gcccgggcac atcacctgaa
    14521 tatgacctac gcctttttct ccgagcattt tcagcgtttc gtcggtataa ggcatcagcc
    14581 agggttcccg cccaaagcgc gactgaaagg tcatcatcac tttttccggt gccatcccca
    14641 gtgcggaagc cagttcgcga gtcgttgtgc ggcaacgttg cggataatca tcgccttcat
    14701 ctgcataacg ctggggaatg ccatgataag agagcagcag cagatccggt tcgccatgtt
    14761 tagcgaaaga agcacgtacg ctgttcgcca gtgcattaat gtaatcgtgg ttatcggcgt
    14821 aatcacgaat aaacgatatc cccggaatgc tacgtttgcg cgccagaatg cgtgccagtt
    14881 catcccatac cgcaccgacc gttgaacagg agtattgcgg ataaagcggc agcaccacaa
    14941 tatgatctac atgctctgcc aggagttcat ctacggcgct ttccagtgat ggcgagccgt
    15001 agctcattcc cagcgctacg ggcgtctccg gtaaacgttg tgccagcgcc tgctgttgct
    15061 ggcggctgta aaccatcagc ggcgagccac cttccatcca gacagaagca tacagcttcg
    15121 ccacacgcgg cgagcgcagt ggcaaaatca cgccgcgtag caatggccac cataacaacc
    15181 gtgaggtatc aaccacacgt ctgtcgctta aaaattgttt cagatagcgt tttaccgctt
    15241 caggtgtggg ggcatcgggc gtacccaggt ttgccagcag gataccggtt ttagtctgac
    15301 gcattaccgc ctcttatcga ttcaacttgt tgataattgt agcggaaaag agaagaagaa
    15361 gaactaattg ctgtgagagg tattgcctga ggaggatgag cagacctgcc cgaaagcaga
    15421 tctgctgcgg cttgattagc cgaggatttt ttccagatca gcgcgaactt cagcaactgg
    15481 cttggtgccg tcaactttcg cgtatttggt gttacccgct tccgcttctt tggagtagta
    15541 gccgatcagc ggtgcagtca tctgatggta ttcaaccaga cgtttacgca cggtttcttc
    15601 ctgatcgtct ttacgggtag tcagttcttc accggtaacg tcgtctttgc cttctacttt
    15661 aggcggattg aatttaacgt gataaacacg accagacggc gcatgaacgc ggcgaccgac
    15721 gatacgatca acgatcagtt cgtccggtac gtcgaattcc agaacgtaat caacattgat
    15781 gcccgcttct ttcatcgcgt ctgcctgcgg aatggtacgc gggaagccgt ccaacaggaa
    15841 accattacgg cagtcttcct gagcaatgcg ctctttaacc agcgcgatca ccagttcgtc
    15901 ggtaaccagt ttgccagcat ccataatgtc ttttgcttgt ttacccagct cggagccaga
    15961 tttgaccgca gcacgcagca tatcgccagt ggagatttgc ggaataccat atttctccat
    16021 gatgaactga gcctgagtcc ctttccccgc gcccggagcg ccaagcagaa tgatacgcat
    16081 tgcgaaaatc cccttaaaat gtgtcgattt ttttgaaaaa gcgataaacg ataccaccaa
    16141 caggcgattg cctcaagaaa ggcacctgag gctgaaacgg ttaatggcga gataatttgc
    16201 ggaaagtgca gatgatgaaa agaaaaatgc cggatgacac gaaggtcatc cggcattaca
    16261 tcaggaaacc agcagctggt tcatacgacg aataaacagg ttcggatctt ccagcgtgcc
    16321 gcgttctgcc agcagcgcct gatccagcag cagttctacc cactcgctga acttcgcttc
    16381 atcttcagta tctgccgcac gtttcaccag tacgtgatcc gggttcagtt cgaagatgta
    16441 tttcacttct ggcactttct ggcccgccgc agcgaacagt ttcgccatct gagtgctcat
    16501 ttcgtccgcg tcggtcgaaa cgatcgctgg cgtatcggtc agacggtgag tcagacggac
    16561 atctttcacg cgctcgccga gcagggcttt cacacggtcg atgaacggag tcagtgcttt
    16621 ctccgcttct ttcgcgctct catcaacttc gtcagccagt ttttccagcg actcgtcaac
    16681 tttagacacc gactggaacg gtttaccgtc gaactcagtc agatagttca tcatccactc
    16741 atcgatgcgg tcggaaagca gcagaacttc gatgcctttc ttacgcagca gttccaggtg
    16801 cgggctgctc ttcgctgccg catagctgtc tgcggtgatg tagtagattt tctcctgccc
    16861 ttctttcatg cgggaaacgt agtcttccag agacacggtc tgcgcagaag aatcggtatg
    16921 ggtagaagca aaacgcagca gtttggcgat cgcttcctgg ttagcgaaat cttccgccgg
    16981 accttctttc agtaccaggc caaactgttg ccagaaggtc tggtattttt ccgcgtcgtc
    17041 tttcgccagt ttttccagca tttgcagcac acgcttggtc agcgcattgc gcaggttacg
    17101 cgttaccgtg ctgtcctgga ggatttcacg ggaaacgttc agcggcagat cgctggagtc
    17161 aatcagacca cgcacgaagc gcagatagtt cggcatgaac tgttctgcgt cgtccatgat
    17221 gaacacacgc tggacgtaca gtttcaggcc atgtttatga tcgcggttcc acatatccca
    17281 cggagcctgg gacggaatgt acagcaggct ggtgtactcc tgcttacctt caacacggtt
    17341 gtggctccag gtcagcggat cgttaaagtc gtgggcgatg tgtttgtaga actctttgta
    17401 ctcttcatcg gtgatttccg acttgttacg agtccacagc gcctgcgctt tgttgatttt
    17461 ttcccaggag ataacggttt cgccatcttt ctcttcacgt ttttcgatct ctaccggcag
    17521 cgcgatatgg tcggagtatt tgctgatgat ggaacgcacg cgccagtcat cgaggaactc
    17581 gtcttcgcct tcacgcagat gcagggtgat ttcagtacca cgatcttctt tggtgatgtc
    17641 ggcaacggtg tattcacctt caccagccga ttcccagaaa acaccgtttt ctggtttttc
    17701 gcctgccgcg cgagtacgca cggtcacttt gtcagccacg ataaaagcag agtagaaacc
    17761 aacaccaaac tgaccgatca gctggctgtc tttcgcctgg tcagaaccca gggattcgag
    17821 gaatgatttg gtgccggatt tagcgatagt ccccagatgg tcaatcactt cgtcgcgggt
    17881 catccccacg ccgttatcgg agatggtcag cgtacgcttg tctttatcga aagagacacg
    17941 aacgcgcagt tcgccatcac cttcgtacag gtcagggtta gatagcgcgc ggaaacgcag
    18001 cttgtccgcc gcatcggagg cgttagagat aagctcacgc aggaagattt ctttattgga
    18061 atagagagaa tggatcatca ggtgcagaag ctgcttcact tctgactgaa aaccacgagt
    18121 ttcttgtcct ttcatgtagg tctacctcaa taatgccatt ttaaggttaa aaacaggatg
    18181 agggagagat ggggacaagg gggaataatt tcaagcgaga gcaggtgatc ctgctctcgt
    18241 ttgcttaaaa acgaatctta tgacgcccgg caagggagtg tgacagcgtg gtgccatcga
    18301 ccatttccag ttcgccgcca accggtacgc catgagcgat tcggctggct tccacgccat
    18361 attgcgcgca aagctcggca atgtagttag cggtagcttc accttcaacc gtggggttgg
    18421 tggcgaggat cacttcagtg attttttcct ctgccagacg ctgttccaga cgatcaagcc
    18481 cgatatcatc cggaccgatg ccgtccagcg gtgacaggtg ccccatcaac acaaaataac
    18541 ggcctgaaaa ctgccccgtc tgctcaatgg cgtagatgtc cgccggactc tccaccacgc
    18601 agatttgacc gttttcctga cgacgcggat tcgaacagat gttacagact tcctgttcgg
    18661 tgaaagtgcg gcaatcggcg cagtggccga tttccgacat cgcccgggtg agcgcctgcg
    18721 ccagacgcat cccgccgcta cgatcgcgct gaagcagcgt gaacgccata cgctgcgccg
    18781 acttcgggcc aacgcccggt agacagcgca gtgcttccat aagctgtgtt aacagcgggc
    18841 tggtttgcat cagaacggca tcttaaagcc aggcggcagc tgcattccgg aggatacaga
    18901 agccattttt tctttctgcg tttcttcgat acgacgtgct gcgtcgttga atgctgcagc
    18961 caccaggtct tccagcatct ctttgtcgtc ttccagcagg ctcgggtcga tctctacgcg
    19021 acggcagttg tgtgcaccgt tgatggtcac ttttaccaga cctgcgccag attcgccggt
    19081 cacttccagc tgcgcgatct cttcctgcat tttctgcatt ttttcttgca tctgctgggc
    19141 ttgcttcatc aggttaccca ggccgccttt accaaacata ggtttctctc tcaatcacgt
    19201 taaggatgac gaacgtaagc tgtgcttacg atcaaatggg gcggatactt tcttcatcca
    19261 gctccgcatc gaagaatcga cgcagggtct gaatattatt atccgcaata atggactcgc
    19321 gcgcctgcgc aagtttttct tcgtatatcg cctgacgcca ttccagcggc gtacgcaccg
    19381 cgggattatc atcttcaacg atagtcagtt caaccgttga accttttaac gtgctcaacg
    19441 cttcagccag tttttgctgt gcaccgcggt tgttcaaatg ccgctgagag gagcgcaaat
    19501 gcagacatac tgcgttgtcg ctctcctctt tccaggcatt taacgccacc tgttcgacca
    19561 gttttggtag cgaaagttgg ctcacctgtg ccgcccacgg gtcgcgctca atggcttctg
    19621 ccgccagctt cgccgccagt tccggcgttt tttcatgttc cagcgctttt tttagcgcct
    19681 tcggcgtggc gaccacttct ttttgctgca tcactggagt ggtggccttc cagcgatacg
    19741 cttctttttt ggctggcgct ttttccagcg ccgatggcac cggacgcgcc tgaacgcgat
    19801 cggtgaccga agccagtctt tccagcgcag cgttattcac cggccgcgcg cgggtagcgg
    19861 ctgccggttc actctttttt gctttggttg ctccctgcac gcgctgcaac tgctggcgcg
    19921 ccgccagcac ctggctggtg gtttccggga gcggtacagt cggtgcctgc tgcggcgctg
    19981 attgcggttg cggcggcacc tgggttggca tgattactgc cgttggcgcg acgggcgcaa
    20041 aggactggcg tggcacttct ggctcaggca gcggcatacg cggatggaac gccagcgcgc
    20101 gaagcagcgt catctcaacg cccatacggc ggtctggcgc atacggtaat tctttgcgac
    20161 caatcaacag cgtctgatag taaagctgaa tatccgtcgg cggtatggtg cgcgccagtt
    20221 cacgcatccg cagctcgatg gcggccatgt cgttgccaag tgcagcaggc gaaagttgta
    20281 ccatcgcaat acgatgcaac aggccgagca tttccaccag caacgcttcc cactcgatac
    20341 cacgggcagc ggcttcatta atcagcgcca ttacgcgctc gccgttggcc tcgaccatcg
    20401 cttcaaccag cgacagcgcc tgatcgtcgt caagcgtacc cagcatcgca ctgaccgcct
    20461 gagttgaaac ctggccgtca ccgctggcaa tcgcctggtc ggtcagactt aaggcatctc
    20521 gcaggctgcc ttcagcggcg cgtgccaaca attgcagcgc ccgcggctcg tgagcgatat
    20581 gttcttcgtt gaggatgtgc tcaagctggt ggcgaatttg ctcgacatcc agcgccttga
    20641 gatggaattg cagacagcgc gacaaaatcg tcaccggcaa tttctgcgga tcggtcgtcg
    20701 ccagcagaaa cttaacgtgc tccggcggct cttcaagggt ttttaacagt gcgttaaagc
    20761 tgtggcgcga cagcatatgc acttcgtcga tcagataaac tttgaaacga ccacgcgccg
    20821 gagcgtactg gacgttatcc agcaggtcgc gggtatcttc aactttggtg cgcgaggcgg
    20881 cgtcgatttc aatcagatcg acaaagcgcc cctgctcgat ttcacgacag ttatcgcaca
    20941 cgccgcacgg cgtcgcggta atgccggttt cgcagtttag tcccttcgcc agcagtcggg
    21001 cgatagaggt ttttccgacg ccacgggtac cggaaaaaag ataagcatga tgaatacgcc
    21061 ctaacgacaa gccgttcgcc agtgcggtca gcacatgttc ctggccgacg acgtcagcaa
    21121 aggtttgtgg gcgccatttt cgggctaaga cctgataact cattggcagg ctctgaaacg
    21181 ctggaaggtg gattcacgaa ggggtaatgc taacacagcc ccgtcagaac ggcgaggctg
    21241 aatgcacatc ttgtgctgtc cgtagcgtgg gcagcacaag actggcgatg attaatggcc
    21301 cgggaacgga acaaggctgt agctggtaat gccctgtttt tcgagacgct gttcgccgcc
    21361 gagatcgaac agattgataa tgaacgcagc gtcagccact tcaccaccca gacgacggat
    21421 cagtttaacg gtcgcttcga tagtgccgcc ggttgccagc agatcgtcca ccaccagaac
    21481 tttgtcgccc ggtttgatgg catcaacgtg gatttccagc tgatcggtgc cgtactccag
    21541 gtcgtaagtt tcactgatgg tctcacgcgg cagtttgccc ggtttacgga ccggtacaaa
    21601 gccaacgccc agacccagag ctaccggagc gccaaacaag aagccacgcg cttcggtgcc
    21661 gacaactttg gtaatgcccg catttttgta acgctcaacc agcaagtcga tgctgagagc
    21721 gtaagctttc gggtcttcca gtaagctggt gacatcacgg aaaagaatgc cgggttttgg
    21781 gtagtcctga atgcttttga tgctattttt gagatactca agctgctgtg cagtcgcggt
    21841 cataagtgta tgcctgcttg ttacggtggt actcacggcg cgttttaaac gtatcaaaag
    21901 taacagttgt ttaacctttg accgcctgcg cctgtgctcg aaaacgccag aatgtactgg
    21961 ctgtacgcaa taattgcaac tgcgattgtt gtgcttcagt gcttttcttg cttttcatca
    22021 atcaccggaa ttcgccacat ataaaaaagc aaacaggcga gaattaccag caacatgatg
    22081 cgcacccatg gcatctggac gaaccacaga gaaatggcaa acgtgagcaa aataagcaaa
    22141 atcgcccgcg gtttgacgcc gcgcggcatc gcatgatgtt tctgccagaa acgtagatag
    22201 ctgccaaacc atgagcggta cagcaaccag gcgtgaaagc gcggggaaga acgggcaaag
    22261 caccaggccg ccagcaggat aaacggcgtc gtcggtaata ccggtaatac cacgcccagc
    22321 gtacccagca ctaccgccag ccagccaatg atgattaaaa tgattcgttg catattgagt
    22381 gttgtcagct tacagagtgg ctactttagc ataacaatta tcattttcat tgaggtctta
    22441 tcgtgaaaac cgccctgctg ctggaaaaac tggaaggaca gctcgctacg ctgcgccagc
    22501 gttgtgcccc ggtagcgcaa tttgccacgc taagcgcccg ttttgacagg cacctttttc
    22561 agactcgtgc gacaacactg caggcttgtc tcgatgaggc aggcgataac ctggctgcgc
    22621 ttcgtcatgc cgttgagcaa cagcaactgc cgcaagtcgc ctggctggcg gaacatctgg
    22681 cggcgcaact ggaggctata gcgcgtgaag ccacagcctg gtcgctacgc gagtgggaca
    22741 gtgcgccacc gaaaattgcc cgctggcagc gtaaacgtat tcagcatcag gattttgagc
    22801 ggcggctaag tgagatggtt gctgaacgtc gagcccgcct ggcgcgggtg accgatctcg
    22861 tggaacagca aacgctgcat cgtgaagtgg aagcctatga agcgcgcctg gcacgctgcc
    22921 gccatgcgct ggaaaaaatc gaaaacaggc tagcgcgctt aacccgctag caatggagag
    22981 agtatgtcac tggaaaatgc ccctgacgat gtcaaactgg ctgtcgattt gattgtgctg
    23041 ctggaagaaa atcagatccc agcccgcacc gtgctgcgcg cgctggatat tgtgaagcgt
    23101 gattatgaaa agaagttaca gagcgatgag gcttcgcaaa gcgagtaaaa aataaagggc
    23161 ggcatcactg tcgcccttta ttgtccttac cctaccgctg gtgtcggatc gtcgcctttg
    23221 tagtcgcgtt ttacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc
    23281 tggttaaagg caatattgat gtcattttca cggcacagct gatcgatagt acggttcagc
    23341 tcatcgacag tacggctacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc
    23401 aacgtgctgg caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt
    23461 gggtgctcag tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag
    23521 gccacgccga gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga
    23581 cgctcggtaa caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc
    23641 gttgtcgcac gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta
    23701 tcgccaatgc gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa
    23761 atttcttgta aaccaaaacc aagacctacg gataatgctg ctgccagcca ctggagttta
    23821 tcccaagaga cgcccagcga cccgaacacc gtcatcgcac caacggcaat aatgatgtag
    23881 ttaaggatgg tagtaatggc atacgacgcg ccctggcgca tattcagtcg cgagagcacc
    23941 agcacttcca gcaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata
    24001 atcgcaaaca acagactgcc catggtgacg tttttcacca ccgcagcacc agcttcagta
    24061 ccgttgtaat gccagagcgt aatgctgtcc agatagctga acacggtgat caaatcggac
    24121 caaattgccc agaacatgac accaaacagc gcaaacatca gcaacatggt aatacgcagc
    24181 gtctgctggt taacctgctc cagtgcaatg gtgggttctt ccggcggttc agcaccttct
    24241 gcgccctctt tcaccagatt ctgccgacgc gccagcgcac gacgccaggc gatacgccgc
    24301 gccgccacgc ttaagccacg cagtactgtc tggtacagca ggttccagat gatcaccaaa
    24361 taaacggttt caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggcg
    24421 gtcagcacca tcaacgcaat cgggataatc gacagtacgg taatggtgac cagtcgcatg
    24481 gtgtgcgact ctttatcacg ccagctttcg cggcacatcg gccataccag gaaggcaatc
    24541 agcagcaggt tgaagaaaat catagcctgc cccagcacat catccatcag atgcagcggg
    24601 gaaagttctg ccaccacaga ccagaaatgg ataggcagca atgcgagact gatgcggaca
    24661 atttgccgac gccagtggct ggtctgctgt tccggcatac caaagtgacg cacggcaacg
    24721 ccgtttttct ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt
    24781 ttactgaacg accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca
    24841 accgcgagaa taatcaggca caccggcagc gcacggatca ggtcgataag gatcgctttt
    24901 ggtgtgttga gctggctgtc gttacgcagg gaacccacag cggaagccag tttttgttga
    24961 tacgctttca gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa
    25021 ccggcgagga aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc
    25081 atcgacttaa attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt
    25141 ggacggttac tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgac
    25201 acactcatta actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc
    25261 aactgtttgt taagctgatc cagcaattcg cgacgcatat caactacttg caataacgca
    25321 tcgtgaactt cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca
    25381 tcgctctgga agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc
    25441 gcgatgcggt tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg
    25501 taaaggatac gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg
    25561 cgttccgatt gaagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac
    25621 tgattaccgt tttcagtcgc tgtaatcaga cgctgactta actgctggtt aatttccagt
    25681 tcctgcttca ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc
    25741 tgcgccgttt tttcggttaa agtcaggcgc ttgctgttta ctgcttcttg caacagctgc
    25801 aactggtgct ccagacgggc gctgttcgcc gtcacgtaat cccgttgctt ttgcaaggta
    25861 tcctgcaaga cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc
    25921 aacgcctgct gggcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca
    25981 tcagtcccat ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca
    26041 ttttgcacgc gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga
    26101 tcgttttgtg cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc
    26161 aacgaaagtg tgctcagaat tttgcgcgtt tcttcgtcat tatcgacatc gctaagtgca
    26221 gttaacgccg cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgccgtagc
    26281 tgaactgtct cttcttttac gcgatcgatt ttatcgaggg tagctaatgt atctgtcaga
    26341 tcctgctgca ccagtttgtc ctgagcagaa agatcttttt gtttattcag tgagtcaagt
    26401 tgtgcctgca ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc
    26461 gtgttctggc ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt
    26521 gatcgtttgt aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg
    26581 acctgaaaaa gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga
    26641 aaatgtccag gaaaaatcct ggagtcagat tcagggttat tcggtaatgg cagggttacg
    26701 aagcgtggga cacaggagat acatctccag taagatggcg acgtaatcgc gggcttcttt
    26761 tttaagatca aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc
    26821 gcgcataata attgctgccc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat
    26881 acaatgtttt aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc
    26941 ctgttgcaca acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa
    27001 taatcgacgc cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa
    27061 tactgagagt ggatcgccag ggaattttgc ctgatactca agctctagtt caccaatatt
    27121 ggattctgac agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta
    27181 gattgcaccg cgcgtaacgc cagctgcttt tgcaatctcg cccagcgagg tggatgatac
    27241 cccctgctgt gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc
    27301 ttcttgtttg gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca
    27361 tttgtgaatg tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac
    27421 ttttgaccat tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga
    27481 gggtttacgc ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt
    27541 gacgacaaac aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc
    27601 aaaactgaac ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc
    27661 gcagaagttc gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc
    27721 gacatcgaag caggtgtctc tctctatcag attgatcctg cgacctatca ggcggcatac
    27781 gacagcgcga aaggcgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg
    27841 gtgaatcgtt atcagaaatt gctcggtact cagtacatca gtaagcaaga gtacgatcag
    27901 gctctggctg atgcgcaaca ggcaaatgct gcggtaactg cggcgaaagc tgccgttgaa
    27961 actgcgcgga tcaatctggc ttacaccaaa gttacctctc cgattagcgg tcgcattggt
    28021 aagtcgaacg tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc
    28081 gtgcagcaac ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc
    28141 ctgaaacagg aactggcaaa tggcacgctg aaacaagaga acggcaaagc caaagtgtcg
    28201 ctgatcacca gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt
    28261 accgttgatc agaccaccgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac
    28321 actctgctgc cgggtatgtt cgtacgtgca cgtctggaag aagggcttaa tccaaacgct
    28381 attttagtcc cgcaacaggg cgtaacccgt acgccgcgtg gcgatgccac cgtactggtg
    28441 gttggcgcgg atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat
    28501 aagtggctgg tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag
    28561 aaagtgcgtc ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa
    28621 gccgcaagcg gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga
    28681 catgcctaat ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat
    28741 gttggcaggg gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc
    28801 gccggcagta acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac
    28861 ggtgacacag gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc
    28921 taacagtgac tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc
    28981 ggatatcgcg caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca
    29041 agaagttcag cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt
    29101 cggcgttatc aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc
    29161 aaatatgaaa gatgccatca gccgtacgtc gggcgtgggt gacgttcagt tgttcggttc
    29221 acagtacgcg atgcgtatct ggatgaaccc gaatgaacta aacaaattcc agctaacacc
    29281 ggttgatgtc attaccgcca tcaaagcgca gaacgcccag gttgcagctg gtcagctcgg
    29341 tggtacgccg ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct
    29401 gacctctact gaagagtttg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt
    29461 gctgctgcgt gatgtggcga aaattgagct gggtggtgag aactacgaca tcatcgcaga
    29521 gtttaacggc caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct
    29581 ggataccgct gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg
    29641 tctgaaaatt gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt
    29701 ggttaaaacg ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca
    29761 gaacttccgc gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tggggacctt
    29821 tgccgtcctt gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct
    29881 cgccatcggc ctgttggtgg atgacgccat cgttgtggta gaaaacgttg agcgtgttat
    29941 ggcggaagaa ggtttgccgc caaaagaagc cacccgtaag tcgatggggc agattcaggg
    30001 cgctctggtc ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg
    30061 cggttctact ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct
    30121 gtcggtactg gtggcgttga ttctgacccc ggctctttgt gccaccatgc tgaaaccgat
    30181 tgccaaaggc gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt
    30241 cgagaagagc acgcaccact acaccgacag cgtaggcggt attctgcgca gtacagggcg
    30301 ttacctggtg ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc
    30361 aagctccttc ttgccagatg aagaccaggg cgtatttatg accatggttc agctgccagc
    30421 aggtgcaacg caggaacgta cgcagaaagt gctcaatgag gtaacgaatt actatctgac
    30481 caaagaaaag aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg
    30541 tggtcagaat accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggtga
    30601 agaaaacaaa gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga
    30661 tgcgatggtt ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt
    30721 tgactttgag ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa
    30781 ccagctgctt gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg
    30841 tctggaagat accccgcagt ttaagattga tatcgaccag gaaaaagcgc aggcgctggg
    30901 tgtttctatc aacgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa
    30961 cgactttatc gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg
    31021 tatgctgccg gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc
    31081 gttctcggcg ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa
    31141 cggcctgcca tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc
    31201 aatggagctg atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac
    31261 ggggatgtcc tatcaggaac gtctctccgg caaccaggca ccttcactgt acgcgatttc
    31321 gttgattgtc gtgttcctgt gtctggcggc gctgtatgaa agctggtcga ttccgttctc
    31381 cgttatgctg gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg
    31441 cctgaccaat gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa
    31501 gaacgcgata cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct
    31561 gattgaagcg acgcttgatg cggtgcggat gcgtttacgt ccgatcctga tgacctcgct
    31621 ggcgtttatc ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca
    31681 gaacgcagta ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt
    31741 cttcgttccg gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat
    31801 cgagcacagc catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa
    31861 gcggcctttt ttatgcataa ccgacgaaca ttaaggagtc attgaaccac caactcagga
    31921 tctc
//
LOCUS       DAYPHN010000037        31895 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000037 DAYPHN010000000
VERSION     DAYPHN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.037"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            45..320
                     /gene="yjfY"
                     /locus_tag="ACG0BW_001696"
     CDS             45..320
                     /gene="yjfY"
                     /locus_tag="ACG0BW_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFK7258670.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEKTDAQGATYYYPILIREPGSNGNMHASADIYR"
     gene            complement(450..1136)
                     /gene="ulaF"
                     /locus_tag="ACG0BW_001697"
     CDS             complement(450..1136)
                     /gene="ulaF"
                     /locus_tag="ACG0BW_001697"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFK7258671.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSTIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(1136..1990)
                     /gene="ulaE"
                     /locus_tag="ACG0BW_001698"
     CDS             complement(1136..1990)
                     /gene="ulaE"
                     /locus_tag="ACG0BW_001698"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFK7258672.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYTHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(2000..2650)
                     /gene="ulaD"
                     /locus_tag="ACG0BW_001699"
     CDS             complement(2000..2650)
                     /gene="ulaD"
                     /locus_tag="ACG0BW_001699"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFK7258673.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRQIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(2664..3128)
                     /gene="ulaC"
                     /locus_tag="ACG0BW_001700"
     CDS             complement(2664..3128)
                     /gene="ulaC"
                     /locus_tag="ACG0BW_001700"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFK7258674.1"
                     /translation="MKLRDSLAENKSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(3138..3443)
                     /gene="ulaB"
                     /locus_tag="ACG0BW_001701"
     CDS             complement(3138..3443)
                     /gene="ulaB"
                     /locus_tag="ACG0BW_001701"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFK7258675.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(3459..4856)
                     /gene="ulaA"
                     /locus_tag="ACG0BW_001702"
     CDS             complement(3459..4856)
                     /gene="ulaA"
                     /locus_tag="ACG0BW_001702"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFK7258676.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWATII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5211..6275
                     /gene="ulaG"
                     /locus_tag="ACG0BW_001703"
     CDS             5211..6275
                     /gene="ulaG"
                     /locus_tag="ACG0BW_001703"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFK7258677.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            6383..7138
                     /gene="ulaR"
                     /locus_tag="ACG0BW_001704"
     CDS             6383..7138
                     /gene="ulaR"
                     /locus_tag="ACG0BW_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFK7258678.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(7135..7884)
                     /gene="yjfP"
                     /locus_tag="ACG0BW_001705"
     CDS             complement(7135..7884)
                     /gene="yjfP"
                     /locus_tag="ACG0BW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFK7258679.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            8066..8395
                     /gene="bsmA"
                     /locus_tag="ACG0BW_001706"
     CDS             8066..8395
                     /gene="bsmA"
                     /locus_tag="ACG0BW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFK7258680.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQSAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            8544..8819
                     /gene="yjfN"
                     /locus_tag="ACG0BW_001707"
     CDS             8544..8819
                     /gene="yjfN"
                     /locus_tag="ACG0BW_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFK7258681.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(8936..10561)
                     /gene="aidB"
                     /locus_tag="ACG0BW_001708"
     CDS             complement(8936..10561)
                     /gene="aidB"
                     /locus_tag="ACG0BW_001708"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFK7258682.1"
                     /translation="MHWQTHTVFNQPMPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAAMPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(10645..11808)
                     /gene="yjfC"
                     /locus_tag="ACG0BW_001709"
     CDS             complement(10645..11808)
                     /gene="yjfC"
                     /locus_tag="ACG0BW_001709"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFK7258683.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(11811..12449)
                     /gene="yjfM"
                     /locus_tag="ACG0BW_001710"
     CDS             complement(11811..12449)
                     /gene="yjfM"
                     /locus_tag="ACG0BW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFK7258684.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(12459..12857)
                     /gene="yjfL"
                     /locus_tag="ACG0BW_001711"
     CDS             complement(12459..12857)
                     /gene="yjfL"
                     /locus_tag="ACG0BW_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFK7258685.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(12875..13534)
                     /gene="yjfK"
                     /locus_tag="ACG0BW_001712"
     CDS             complement(12875..13534)
                     /gene="yjfK"
                     /locus_tag="ACG0BW_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFK7258686.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAITLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTIGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(13585..14283)
                     /gene="yjfJ"
                     /locus_tag="ACG0BW_001713"
     CDS             complement(13585..14283)
                     /gene="yjfJ"
                     /locus_tag="ACG0BW_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFK7258687.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(14302..14703)
                     /gene="yjfI"
                     /locus_tag="ACG0BW_001714"
     CDS             complement(14302..14703)
                     /gene="yjfI"
                     /locus_tag="ACG0BW_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFK7258688.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNGENALIIKMNDYGDLQINIL
                     FTSCQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQHEEYYIVFGALS
                     LKSSLKDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(14831..15562)
                     /gene="rlmB"
                     /locus_tag="ACG0BW_001715"
     CDS             complement(14831..15562)
                     /gene="rlmB"
                     /locus_tag="ACG0BW_001715"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFK7258689.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(15653..18094)
                     /gene="rnr"
                     /locus_tag="ACG0BW_001716"
     CDS             complement(15653..18094)
                     /gene="rnr"
                     /locus_tag="ACG0BW_001716"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFK7258690.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFSGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(18133..18558)
                     /gene="nsrR"
                     /locus_tag="ACG0BW_001717"
     CDS             complement(18133..18558)
                     /gene="nsrR"
                     /locus_tag="ACG0BW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFK7258691.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(18763..20061)
                     /gene="purA"
                     /locus_tag="ACG0BW_001718"
     CDS             complement(18763..20061)
                     /gene="purA"
                     /locus_tag="ACG0BW_001718"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFK7258692.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(20165..20362)
                     /gene="yjeT"
                     /locus_tag="ACG0BW_001719"
     CDS             complement(20165..20362)
                     /gene="yjeT"
                     /locus_tag="ACG0BW_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFK7258693.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(20444..21448)
                     /gene="hflC"
                     /locus_tag="ACG0BW_001720"
     CDS             complement(20444..21448)
                     /gene="hflC"
                     /locus_tag="ACG0BW_001720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFK7258694.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(21451..22710)
                     /gene="hflK"
                     /locus_tag="ACG0BW_001721"
     CDS             complement(21451..22710)
                     /gene="hflK"
                     /locus_tag="ACG0BW_001721"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFK7258695.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(22796..24076)
                     /gene="hflX"
                     /locus_tag="ACG0BW_001722"
     CDS             complement(22796..24076)
                     /gene="hflX"
                     /locus_tag="ACG0BW_001722"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFK7258696.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(24153..24461)
                     /gene="hfq"
                     /locus_tag="ACG0BW_001723"
     CDS             complement(24153..24461)
                     /gene="hfq"
                     /locus_tag="ACG0BW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFK7258697.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGSTSSNYHHGSSAQNTSAQQDSEENE
                     "
     gene            complement(24547..25497)
                     /gene="miaA"
                     /locus_tag="ACG0BW_001724"
     CDS             complement(24547..25497)
                     /gene="miaA"
                     /locus_tag="ACG0BW_001724"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFK7258698.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(25490..27337)
                     /gene="mutL"
                     /locus_tag="ACG0BW_001725"
     CDS             complement(25490..27337)
                     /gene="mutL"
                     /locus_tag="ACG0BW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFK7258699.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRIAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDDPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RGGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(27347..28684)
                     /gene="amiB"
                     /locus_tag="ACG0BW_001726"
     CDS             complement(27347..28684)
                     /gene="amiB"
                     /locus_tag="ACG0BW_001726"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFK7258700.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(28703..29164)
                     /gene="tsaE"
                     /locus_tag="ACG0BW_001727"
     CDS             complement(28703..29164)
                     /gene="tsaE"
                     /locus_tag="ACG0BW_001727"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFK7258701.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(29136..30683)
                     /gene="nnr"
                     /locus_tag="ACG0BW_001728"
     CDS             complement(29136..30683)
                     /gene="nnr"
                     /locus_tag="ACG0BW_001728"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFK7258702.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFLVCRTAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRVVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVLVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            30682..31821
                     /gene="queG"
                     /locus_tag="ACG0BW_001729"
     CDS             30682..31821
                     /gene="queG"
                     /locus_tag="ACG0BW_001729"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFK7258703.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(31804..31857)
                     /locus_tag="ACG0BW_001730"
     CDS             complement(31804..31857)
                     /locus_tag="ACG0BW_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258704.1"
                     /translation="MRFYFYSQAVDESGITR"
BASE COUNT         7632 a         8890 c         7839 g         7534 t
ORIGIN      
        1 atcacagagg agagcacaca aagtcagccg ttgaggaggt atctatgttc agtcgtgttt
       61 tagcccttct ggctgtgctt ttgctaagtg caaatacatg ggcagccatt gaaattaata
      121 accaccaggc cagaaatatg gacgatgtgc aaagcttagg cgtgatttat atcaaccata
      181 acttcgccac tgaaagtgaa gcacgtcagg cattaaatga aaagacagat gcgcagggcg
      241 caacgtacta ctatccgatc ctgatacggg aaccgggaag taacggcaat atgcacgcca
      301 gcgcggatat ttatcgctag caccaggtat aaccaacgaa acattgccat agtttgcttt
      361 gcccccttcg cagggggctt ttttttgaaa aacggctgcg gaattagacc agttatctcc
      421 cgaagaagga aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttgggacca
      481 tgcttacgca tgaagtgttt attcatcagg tagctgtcga tgtgattcag ttgtgggtta
      541 atgccgcggg caatccacgc catttttgcc acttcttcca tcaccaccgc attatgcacc
      601 gcatcgtggg catctttccc ccaggcgaac ggcccgtgct ggtacaccac aatccccggt
      661 gtatgcagag gctcggcgtt gcccagcgtt tcgataatca ctttgccggt gttcagttca
      721 tactcgccct gcacctcttc ttcgcttaac ccgcgcgtac acgggatatc gccaaagaag
      781 tagtcggcgt gcgtggtgcc taacgccggg atcgccagcc ccgcctgcgc ccatgcggta
      841 gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg gataacgacg gtagagttcg
      901 agatgcgtcg cagtgtcgga agacggacga tactcccctt ccaccacctt accgctcata
      961 tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg cgacaccgct gggcttgatc
     1021 accaccagcc cgcgttcgcg gtcaatggtg ctgacgttgc cccaggtaaa ggtcaccaac
     1081 ccatagcgcg gcagatccat gttggcttca aacacctgct gttttagctt ttgcattatg
     1141 ccgcctccac catgcctgct ttggccatgc gcgctttcac ccaatcacgc gctttcgcca
     1201 cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat
     1261 agccactctg tttgagcgtt tcgaagcaac gttcgaaatc cactacaccc tcaccaaacg
     1321 gcacgttttt gaagacgccc ggtttggtgt ctttcacatg caccgcgacg atatgcccga
     1381 ttccggcctg caactccatc tgcacgtcgt tatcccacgc cgacaggttg ccgatatccg
     1441 ggtagagctg gaaccacggg ttgttgagat agtgcgtgta acccaacgcc ttactgatgg
     1501 agttcatcaa cgggtaatcc atgatctcca tcgccagcgt cacctgcgcg cggcttgcca
     1561 tctcaacgct ctctttcagc ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct
     1621 gatagtaaac gtcatagccc gccagctgga tcacgcgaat accgacgtcc tgggcgaact
     1681 ggatagcttt gcgcataatc tccagcccct gcgcccgtac cgcgtcatct tcgctgccca
     1741 gcgggaaacg acgatgagca gaaaggcaca tggacggcac gcgcacgcca gtttcaacaa
     1801 tcgcattcac cagcgccaga cgctgctcgc ggctccagtc gaggcgcgac aggcgatcgt
     1861 cagtttcatc taccgacatc tcgacaaaat cgaagcctaa cgtttttgcc agttgcagcc
     1921 gttccaacca gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt
     1981 tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgcg
     2041 cggcttcaac cggagaagcg gcatcacgga tactgcgacc agcgataaac acgtgaatcg
     2101 gaatgccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg gtcactttga
     2161 agcccatatc ggaaagacgt ttgatcgcgg taatgtccgc ttcgccccac gccacgcctg
     2221 cggcctgtgc gtcacggctg cggtgataaa ccacctgttg aatgcctgcg tcgcgccact
     2281 gttgcgcctg ttcccaggtc cagtagccgg tcagctcgat ctgcacgtcg ccgttaaact
     2341 cttttgccac gtccagcgca cctttggcgg tgttgatatc cgcacagcaa atcacggtca
     2401 cccagtcagc gttggcttca aagcacatac gagaaaggat tttgcccgca tcggcaattt
     2461 tggcgtctgc cagtacgatt ttgtgcgggt aaagcgcttt cagatcgcga accgcacgca
     2521 cgccttcgcc cacgcacaga atggtgccca cttcgataat gtcgacttct tcggcaatct
     2581 ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgacc tgcaacatcg
     2641 gtaatgacat tttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt
     2701 acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tctcttcatc ttcaaacaga
     2761 ttgacgatct gcatgatgcc cacttcctgg tgagtgttgg catcgaccgc cgccatggtg
     2821 atgaggatat ccaccggatc gttatcttcg tggttgaact ccagcggctt tttcaacgtt
     2881 accagagaga aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg cattgccagg
     2941 cctggtgcga taacgaaata aggaccaaac tgttcaacgc catccagaat cgcctggtag
     3001 taacgcggct ctaccacatc tgccgctacc agcaggtcaa caccgatttt caccgcttcc
     3061 tgccaggtat ttgcttcggc ctgcaggcgg atagatttat tttcagccag cgaatcacgt
     3121 aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc
     3181 agtttcgggc caaagtcagc aggagagagc atgttgcgca cgccaaccac atatttgttg
     3241 ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc
     3301 aactcgcttt tgtattcgcc aaccgcgcag ctgtttaccg tgtggtcaat gtttgattgg
     3361 gttaaaaact ggtccacttt cattttcatg atcatggaac tgccttgtcc gttgccacac
     3421 acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccagt
     3481 tgtttttctg catcttcttc tgcgcgcagc gcgcggccag cgaagaacat ataagccagt
     3541 gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc
     3601 ggtggtgcca gaatcgacca gtccgccatg cccatccagg cactcatacc ggtgagtttc
     3661 accgcccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc
     3721 gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc
     3781 gggataaagc cgggaataat caggatcgag gaaccgcagg cgaccaggat gccaactgca
     3841 atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg cgcgaagcta
     3901 tagatagccg cacagtcaat cgccagaacc gcacctggga tcaggcgctg ggaaatgccg
     3961 ttaaatgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc
     4021 gccaccgcga aggagaaacc agtttgcagg atgtagaccg tccagttcac tttgcctgcc
     4081 atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg
     4141 acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg
     4201 tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc aatccatgat
     4261 gcaaactgtt gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc
     4321 ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata
     4381 atggtcgccc acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc
     4441 tggaacatga tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc
     4501 acgtaacaga tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca
     4561 tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta
     4621 atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg
     4681 gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg
     4741 ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacggg
     4801 gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg
     4861 atctcctgaa gaagaggtaa ccgggtattc cacacacccg gaatgttgtg cactcactct
     4921 aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat
     4981 aattcctcat ggatagagtg ggattcacac aacgaaataa ttattcattt aaattcattt
     5041 agttaattga attacacatt ccattaaatc tttccacact caaaatcaac aacaacttcc
     5101 ctggcaaaaa gtcaaaaaat ggtcattgcc ttcctgaaaa aatagtgtca ggattaatca
     5161 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
     5221 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
     5281 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
     5341 gctgcaccgg gatctggctg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
     5401 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
     5461 gcatggctgg cgtgaaaaaa ctgcagccaa acctgcgcac caccccgttt gttcttgatc
     5521 cgtttgcaat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
     5581 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
     5641 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
     5701 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
     5761 accgtactgc actgatcacc ctaccagcgg atcaaaaagc ggctggcgta ctgccagatg
     5821 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctgtatcaca
     5881 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
     5941 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
     6001 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     6061 tctggtcaaa cttccaggcc gatccgcaag aaatccgcgt gctgtgggag atgaaaaaag
     6121 atcgtctgaa gtatggcttc aagccgttta tctggcaggt gggcggcaaa tttacctggc
     6181 cgttggataa agacaacttc gagtaccact atccgcgtgg tttcgatgat tgcttcacta
     6241 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     6301 gagtagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     6361 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     6421 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     6481 tgccactgcg cgacgcgata tcaataaact cgacgaaagc ggcaaactga agaaagtgcg
     6541 caacggcgcc gaagcgatta cccagcagcg cccacgctgg acgccgatga atctgcatca
     6601 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     6661 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     6721 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     6781 ggagcatgac agcgtgatca ttatgggcgg acaatacaac aaaagtcagt ccatcacttt
     6841 aagcccgcaa ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggcaa
     6901 agggctgaca gcagaagggt tgtataaaac cgatatgctg acggcaatgg cagagcagaa
     6961 gatgctaagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     7021 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     7081 taacccagaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     7141 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     7201 gcctggctgc catgaacagg ttagcagttt atctcgtccc gtctcgctta aggcctgctg
     7261 caaacgtagt gattcgtcgg caggcacgac gtcatcgtca aggccatgcc acagcagtag
     7321 aggtctgtca ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     7381 aatgttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     7441 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     7501 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     7561 acggtcatca cgcagccagt tttcttcggc tattgccgca cgtaaggtag tgaattcctg
     7621 catactttgt agcaagattt gccagaattg atttaaccgc cgcgctgcgt cgccactaaa
     7681 acgactaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
     7741 aacggcaaaa tagctataca ccagactgga cgaagtaaag ccgtgataaa aaatcgcgca
     7801 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
     7861 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
     7921 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
     7981 ttcatcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
     8041 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
     8101 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
     8161 acagtcagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
     8221 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
     8281 agaaatcaga gcgaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
     8341 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
     8401 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
     8461 aaattaccgg ctggaagctc gggcgtttat taccgcgatg gaatgccctg caacatgtgg
     8521 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
     8581 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
     8641 caggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
     8701 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
     8761 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
     8821 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctataa
     8881 ttttcacata tttcattgag ttaattgaaa ccagcgttgc atcagtcgta tacgcttaca
     8941 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
     9001 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaggcat
     9061 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
     9121 gttcttcagc aggcttacgc agctgctgct gtaaacgacg taccgcgcga tcaaaatagc
     9181 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtataca cccgcttgct
     9241 tattgagcac gcgcagtaca tccaggcaca taatattacc ggaaccttcc caaatactgt
     9301 ttaccggcat ctcccggtaa agccgcggca gctcgctctc ctcgcaataa ccaatgccgc
     9361 ccagcacctc catcgcttcg gccacaaacg gcatacctcg tttgcagatc acaaatttcg
     9421 ccgcaggcgt aaataaacgc gcccacagag cttctttggc atcggcacgc cggtcccacg
     9481 ctcgcgcaag acgaaacagc aacgccgttt gcccttcaag ctgaagtgcc atgcgactta
     9541 agacatgacg cataaggggc tgttggatca atggattacc aaaaacatgg cgttgatgtg
     9601 catgataaat cgctagcgaa aatgcacggc gcatcatggc atggctaccc agggcgcaat
     9661 caaaacgcgt catcccaccc attttcagga tcagacgaat tccttcccct tcctgcccca
     9721 acaaccagcc aatggcatcc tgaaactcca cttcgcagct ggcgttagag cgattaccca
     9781 gcttatcttt cagccgctcg aggcgaatcg cgttgcgttg cccgtcaggc aaaaagcgcg
     9841 gcacaaaaaa gcaggacaaa ccgcccgtag tctgcgccag caccagatgc gcatcgcttt
     9901 gcggcaccga gaaaaaccat ttatgcccca ccagccgata agagccatct tccaggcgct
     9961 ctgcgcgggt ggtgttgctc atgacatcgg aaccgccctg cttttccgtc attcccatgc
    10021 caatcaacaa accgcgtttt tgcccacctg gcaataagtg agaatcgtag cgatcgctca
    10081 acagcggcgt ggtccagtcc tgaaacggcg cgggtaacat ctgtaacagc aatggcatgg
    10141 cggcaaaggt catcgttatc ggacataacg accctgcctc aacctgtgcg tgcaacataa
    10201 aacgcgccgc gcgcgccaca aatgcgccgg agcgagcgtc ttcttcccag gcaagattgt
    10261 gcacccgatt ggtacatagc gcctgcatca gcaggtgcca ggcggggtga aaacgcacat
    10321 cgtccagacg ccgcccctgg gcatcgtagc gcaataattc aggcggattc acattcgcca
    10381 gccgccccag ttcaagggat tcagccgtcc ctaactgctg accaatactg gctagaaaat
    10441 cgctatccca gccagcacct tcacgcgtta ccgcttcgca gagcgcgcca tcagacaggt
    10501 ataagttgct gttatttaat ggcataggtt gattaaaaac ggtgtgagtt tgccagtgca
    10561 ctgtgtctcc ctccatcaat ggcagtcacc attagtatgg tcactgccat ggattcatga
    10621 cagaaagaga ttgctaaaac attcttatcc agcaatgtaa tgcggaacga aacgtgaggt
    10681 gtctttggtg atcagcgtgt tatcttcacg gatccccatt ccgcacgctt catcatcgac
    10741 aatccagcta ccgatgagtg tgtagctatc gccaaatcgc ggcagaggtt gaaacgcctg
    10801 gtagatcatc ggttcatcgg cgtaatcacc atcagcgtgg tcaacgacgt tattctgacc
    10861 gtcgaaaatg gtgacattgc cgccttcgcg cgagtagatc ggtttacgca catagctttc
    10921 gccagcggca atctgcgatt tttcaccctc aaaccaggat gcaagaaggt taggatgacc
    10981 agggaagaag cgccaaagca gtggcattag ccctttatta ctcaagatac ttttccataa
    11041 cggctccacc cattgctcgc gacgcttgcg cagcagcgga ccgttatcgt cacgcatcat
    11101 ccactctagc ggatacagct taaatgcacg ctggatgaca ttatcatcaa gatcggtcag
    11161 tacgccgccg acgcccaaac cgagatcttc aatgtagata aaccgcgact cctgccctgc
    11221 ctgctgggcg cagtcctgca agtacagcac ggtactcctg tcttcatcgg tgtcctgaca
    11281 gcagcaaaaa taaaacggtt cccgactgta aagctcgcta aagcgtgaaa tcaggcgttc
    11341 ctgaatggca ttgtactgat cggcatcacg cggaataatg ccgctgcgcc gggcatcctc
    11401 cagccacagc cactggaaat aagccgattc atacaacgag gttggcgtat cggcgttgta
    11461 ctccagcagc ttcaccggcg cattgccaca ccaggcaaaa tccatccggc catacagcga
    11521 aggatcgcgt gcgcgccagc tttcagcgat cacatcccag tacaacggag ggatcgccag
    11581 ttgcgtcagg atctcttcgt ctttaaccgc gcgatccacc acctcaaggc acatctgatg
    11641 cagttccgca gtcggttttt cgatctgctc ttcaatctgg cgcagagtaa aacggtaagc
    11701 ccgactctcg tcccaataga tttcattgtc gatgatatga aagtcgaaac cgttgtcagc
    11761 ggcgatctgg tccagatccc gccgcacagg aacgttgtgc ctcagcatga ttagcctccc
    11821 caatgcccac gggcgctgga agaacgacca tagccgccgc gagaaacggt tgacgctttt
    11881 ttggtggtaa agccgcccga agagtaagac tcttttttgc cagaaccgga gcgccaggag
    11941 taatcaccag aagtgttgcg ccagacgggg cgcgaagcaa aagaggaacc gccgctgtta
    12001 taaacaaact gctcatcacg atctttacgt ataacccggc ttaacaaaaa tccagaaacg
    12061 accgggatcc aactctgttc aacgttgtcg tagtaacaat tttcatactt agactgacaa
    12121 ttctgctgag tcatattctt cggaacatcg gcataaaacg ccgctttggc gttgttccag
    12181 ccatgagcac agatatctga attattaccg tcatcaatac aatcctgcac agtcgagtaa
    12241 aacgttccgt cgccgtcgtt atcgacatcg ccactatcgc cacaaccttt caatacgaaa
    12301 aaagcggcac cgcccattag cgccaacgtt aagtattttt gtgcgtaacg attgcgacgc
    12361 ggttcaaaag gattattcgg tctaccaatg cgactgatcg ctccgtgacc aattttactg
    12421 ttatttcttg atttgcgttt tctggccatg atcgtccatt accatgtcat acaagctgcg
    12481 ttaaaaatac cgccagccag tgcggcggtt cccataaaca ttcctgctgc ggtattgtga
    12541 ttaataattt tttcgcttaa tgcgggcata taaagtctga cgccagcaaa aacgagtaac
    12601 tgaatcacca gtgcgatccc gccccaggcg aaatagtctg gaatactcac cgcattgatt
    12661 gccgcactgg ataacgggat cacgtaaccc aacaatgtac cgctgaacgc cagtgacgcc
    12721 gcggtattat tgtttttgat taactgccat tcgttgtgcg gtgtaatttt ggagtagata
    12781 aacagaaaaa taatcaccat agccacgcca ataaaaaaat aggcactaaa ggcaagaagt
    12841 gaatccagta tatgcattgt aaacgtcctt gtaattaacc aataatatga agtgatgtca
    12901 gcgggatatc gacacccaac gcgcgcgaaa ataaccactc tggctcgccg agatcgttaa
    12961 aagattcctc accatttagc agcaaatatt cgtaagtatc ttcggttact tgccgttggt
    13021 agcctatggt aaaattatgg acctcccatt tggcatggtt ttgattttct actttttcca
    13081 gcatgtagac gggttcgata tttcccggct cttcactgtt aaaaaatcgc tgccagcgtt
    13141 tttcctgcca gtttaaggtt atagccccca tcgctttggc gttgatcgcc tcgcgccagt
    13201 ggctctcttt actgataccg tagctctctt catagacaaa gagcttaata tcatcaatgt
    13261 catcaatatc ttcaccgccg gtggtattga tttgcagaaa ttcatcgccc gaagtgtagt
    13321 agcggaaaat ctggctaccg ccgcccagat cgatgcggct gacggcagct accgtaaatt
    13381 cctcacccgg cagcgcaatc agcaattcat cttccagcaa acgaaacgct aacgtgtcga
    13441 gcgtaaagcc actattgaga tgaagtccca gaggaccgcg agcgatagca ggcttattat
    13501 ccttgccaaa cagacgctgg aaaaaaccag acatatttta tccttaaaat agttgccagc
    13561 cttgcagtga ggctggcaaa aaaattactc gccctgttgg cgttgcagtc ttgctaatac
    13621 atcctgggcg ctacttttat tgctaccgcc tattccggct tccgccagct tttcatcaag
    13681 atcgcgaccg tctgcgactt tttccaactg tgcagcagca tccaggcgag cctgacgttc
    13741 ggcctgacgc gtttgcaggc gttttaagga ttctgccgct gtcgaaacgc tggaagatgc
    13801 gccaacggta gaggtcgtta cagcctgttg tgcacgctgc atggcttcag tagctttaac
    13861 gacttccatt tgctgctcaa actgagcgat acgctgcgct gtcgctgtaa ctgctttttc
    13921 cacgccatca cgagagactt ccagattcga caacacttgc tcttcagcgg taatgagatt
    13981 ctcaaggcgc gcgatttctt cagcaacttc gttaatcaac gacggattaa cgttcttgct
    14041 taacgcttcc agcgcacggg cttccagact ggctttgcgc tcacgtaaat ctttcagctt
    14101 atcgtgactt aatttcaccc gcgccagcag atcaacgcga gattttccgg ctttatcgag
    14161 ttcagcttta gcgtcacgaa tatgctgttc cagcatgcgc acgccctggg tttcttcaat
    14221 ggattcttcc gcctgagaga taaacgattt ccccagcgta aataaacttt ttaaaattcc
    14281 cataaatacc cgtccttgta attagtgtga atattcttct gtaatttcag ccagatccaa
    14341 tgcgttatct accagcgagg ttatctcaag caggatatct ttaagagaag atttaagcga
    14401 taacgcaccg aaaacaatgt aatactcttc atgttgcacg ctggagatcc ctaccgatga
    14461 caacggcatc atcttctgat ttcttaataa gaaggtatta aattcatctg gattgctgat
    14521 actacttacc ggacaaataa aggtttcgat aatcatttgg caggaagtaa atagaatatt
    14581 gatttgcaaa tcgccataat cattcatttt aataattaat gcgttctcgc catttgttac
    14641 atcaatattt tgttcaggta cagtttgcag cgccgtcgcc agcgccaacg gattccatgt
    14701 catatattat tccatataaa ttaagtttaa atattaataa aatgaatatt tgcagtacgt
    14761 aattatctta ccagctatag acaaaaaaaa aaccatccaa atctggatgg cttttcataa
    14821 ttcagaggaa ctagctgcgc tgtcgcaccg cttcaaataa gcaaatccct gtcgccacag
    14881 aaacgttcag ggaagaaacg cttcctgcca ttgggatgct gatcaactcg tcgcaatgtt
    14941 cacgagtcag gcgacgcata ccttcacctt ccgcgcccat caccagtgcc agccgtccgg
    15001 tcattttgct ctgataaagt gtatgatccg cctcacctgc cgtaccgacg atccagatat
    15061 tctcttcctg caacatacgc atggtgcgcg caaggttagt cacccgaatc agcggaacac
    15121 tttctgccgc gccgcaggct acttttttcg ccgtggcgtt gagctgtgcg gagcgatctt
    15181 tcggcacaat caccgcatga acaccagcgg cgtccgcgct acgcaggcac gcgccgaggt
    15241 tgtgcggatc ggttacaccg tcgaggatca gcaggaacgg ttgatcgagc gaagcgatca
    15301 gatccggcag atcgttttcc tgatactgcc gtcctggctt cacacgggcg ataatgccct
    15361 gatgcacggc accgtcgctt ttctcgtcga gatactgacg gttcgccaac tggataacca
    15421 cgccctggga ctcaagggcg tgaatcagcg gtaacagacg tttatcttca cggcctttta
    15481 aaataaagac ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc actgcgtgga
    15541 tgccgtaaat catttcgctc attaatgtac tcgttgttta cgttttgccg ggagcgccac
    15601 gcttgcccgg cctacggata attcaccctc ttctgtttga agagggtaat gatcactctg
    15661 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc tgcgttttcg
    15721 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
    15781 caccgctgaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc
    15841 cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
    15901 gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt acgctcgtcc atattaaccg
    15961 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattcaccca
    16021 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
    16081 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
    16141 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
    16201 cagctacatc gcgtgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
    16261 gttgcagcat ctcttccatc gaataatggt aaccgccggt ttcagtagtg ttgccctgat
    16321 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
    16381 gacgaatcgg cgaagtgaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
    16441 tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc agcatttctg
    16501 cgtcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt tccggcttgt
    16561 taccgcccgg caattccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
    16621 cggtgctcgg cttatcgtga atacggaata gtgctggttc tttggctttc tcaacgaaac
    16681 gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt gcgtcgttac
    16741 gctgagtctg ttcgatacgc tcaatacggc gttcggcgtt gaaaatgaac ttcgcttctt
    16801 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
    16861 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
    16921 cctgcagaat atgccagact ttggtgtagg tcagacgtgc gtgagagctc attaccgctt
    16981 catagaattt gtagccagtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
    17041 acaggcggtc tacctgcggg ttaagcgaac acagaccgtt agagagcact tccggcagca
    17101 tcgggataac ctgcgaaggg aagtacaccg acgtaccacg gttacgcgct tctctgtcca
    17161 gcggcgttgg agggcgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
    17221 cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg tcttcgccat
    17281 caatggtgac cagcggtaaa tcgcgcagat caacacggcc cgcttttgct tcttccggca
    17341 cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg tacggaattt
    17401 catgggtacg cagagcaata tcaaccgcca tgccggtgcc catattgtcg ccaagtactt
    17461 cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt tcaaccacca
    17521 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
    17581 aacgactgtc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
    17641 tctggctggt ttttggtacc agtacgcgga cgatacgcgc ttcacgacga cctttacggt
    17701 cagcgcccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
    17761 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
    17821 ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
    17881 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
    17941 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
    18001 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
    18061 cagcttcgcg ttcctggaat ggatcttgtg acatctcggt tcctccgttg ccatctctga
    18121 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
    18181 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
    18241 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
    18301 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
    18361 acgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
    18421 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
    18481 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
    18541 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
    18601 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
    18661 cgaggattta acactgtgtc ggatagcgta atggcttacc cgacacagca atgtatcaca
    18721 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
    18781 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
    18841 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
    18901 tttcacgccg aaggtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
    18961 tttccagtcg tcagctgcca gcggagtggt agtcacttca cgaccatccg gcatacggta
    19021 agccacgcag agtttcacct ctttcaggcc atccagaacg tccagcttgg tcaggcagaa
    19081 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
    19141 acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc tgcttgcaga ggaactcgcc
    19201 agtttcatca aacagttcag tcgggaacgg acctgcaccc acacgagtgg agtaagcttt
    19261 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
    19321 accagcagtg gtgttggaag atgttacgta cggataagta ccgtggtcga tatccagcag
    19381 cgtaccctga gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
    19441 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
    19501 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
    19561 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
    19621 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
    19681 gatcgctttc gcgccacgtg ctttctcacg cgcgttatcc agcgcaacgt gataatcaag
    19741 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
    19801 cagttctttc atctctttca tcagcgcggc tggagacagc acaacaccgt taccgatgat
    19861 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
    19921 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
    19981 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
    20041 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
    20101 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
    20161 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
    20221 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
    20281 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
    20341 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
    20401 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
    20461 tcggcgtctt catgtagcgg aagaaatcgc tgtccgggct catgaccatc acgtcctgat
    20521 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
    20581 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcatga
    20641 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
    20701 gcagtttttc agcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
    20761 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
    20821 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
    20881 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgccg
    20941 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
    21001 cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg cgaccaattt
    21061 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
    21121 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
    21181 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
    21241 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagacccg
    21301 gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
    21361 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
    21421 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
    21481 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
    21541 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
    21601 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
    21661 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
    21721 cgtctcgata tacagacgct cgcgagtaat ttccggcgca gctttatatt ccggcagaag
    21781 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
    21841 tgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata
    21901 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
    21961 cgccgctttt acttcttctg gcggacgagc tgcctggaag ttgacgtcca gcagagtgat
    22021 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
    22081 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc cacgcagggc
    22141 gctgtcggta gcctgacgca gactgtcatc cgggctggtc acgctataca gatatttttc
    22201 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
    22261 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
    22321 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
    22381 gcgtgttacc acgccacgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
    22441 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
    22501 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccaagctt
    22561 tttgctcagc ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
    22621 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
    22681 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
    22741 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
    22801 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
    22861 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
    22921 gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
    22981 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
    23041 tctgtgcgga aagccagaca cggatcggtt tgttctcttc atcccgatca atacgcggtt
    23101 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
    23161 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgt acatccgccg
    23221 catcaatgac gtgcagcagc aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
    23281 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgcc aacacggttt
    23341 caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
    23401 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
    23461 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
    23521 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
    23581 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
    23641 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
    23701 gctcaacctg caacttaccc tcatgggtac gggcacgttg ggcgaaaata tctaaaataa
    23761 ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
    23821 ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca gcttccgcaa
    23881 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
    23941 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
    24001 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
    24061 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
    24121 ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgt tttcttcgct gtcctgttgc
    24181 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtact gccaccggcg
    24241 ttgttactgt gatgagaaac cgggcgagac gggacaacag tagaaatcgc gtgcttgtaa
    24301 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
    24361 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
    24421 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
    24481 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
    24541 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
    24601 tttttcacta tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
    24661 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg atatttcgcc
    24721 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    24781 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    24841 atgaaaacgc tgctcaattc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    24901 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    24961 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    25021 ccttgctgcc gcaaccggat cgatctcctg aagttgacga tgcaatgact cccaaccttg
    25081 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    25141 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    25201 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    25261 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    25321 agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag aatcaacgct
    25381 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    25441 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    25501 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    25561 tcacaagttg cggacataac cgttccacgt ccgccagcag agttatggcc tgcgccattg
    25621 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    25681 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    25741 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    25801 caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    25861 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aactggcgct tcacccggcg
    25921 tcagttgcgc ctgacgcagc caacgttctg ccactggcaa ggctaaaagt gaaatgttgc
    25981 cgccgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    26041 gactgttcgc cgcaagtgca ggttcctgcg gttctggcgc ttttggtttt tgcatcggcg
    26101 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    26161 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagtg taacgcggag
    26221 ctactggttc acgaactgcc ggttcagcaa agtgattgcg ccccgccgcc acgcggtttt
    26281 ccggaatggc acgcggtgca ggttgggggt catcgtccag cggtagcggc gtttccagtt
    26341 gctgttgtag cacgctcagc acaccctgat agataaagtc atgcaccaga cgcgactgat
    26401 ggaaacgcac ttcgtgtttg gcagggtgca cgttgacgtc cacctgatgt gggtcgatct
    26461 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttca caggcctggc
    26521 ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc attcacatag caatactgaa
    26581 tttccgccag tgcgggcgtg gtgtgattcg gatctgccac ccagccgcgc agcgtaagat
    26641 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    26701 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    26761 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cgaataattt
    26821 catcgatatg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    26881 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgccgcc ggtttaaccg
    26941 ttacgtccat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt tctgcgatgc
    27001 gtgaagtgag cgtcaggcgc gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    27061 caaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    27121 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    27181 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    27241 tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    27301 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    27361 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    27421 gcatcggatg cgcaaggaaa taattacgca ggcctttgta aatggcttct gccagctgtt
    27481 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    27541 cgaccagtac tgatgggata tccggcgaac gcagaacgcc aaggctggcg tgttctggtc
    27601 gacgtttatg tatttcgcca atgcgttgca actggctgat catactggtc gctacatcat
    27661 accctacacg ctgggaatgg ccgaactgta aatccagcac agcctggctt aaataggggt
    27721 cagactgact gttcgccagc acatcgcccg ctccgcccaa tagctccgac tgtttctcat
    27781 gctgttccag ccagcttgcc atctcgctgt ttgcacgacg gttagagagc acccatacgg
    27841 aagcgccagt cgcactgcgg tttggtgcgg catcagcgtg aatcgacacg aggaaattgg
    27901 cgttttgctt acgagccaca tcgctgcgcc ccatcaccga gataaagtaa tccccgtcac
    27961 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    28021 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca ggatcctgac
    28081 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc tgtgttagcc gtcgcgcgcg
    28141 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    28201 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    28261 gtttcgcaac cacaggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    28321 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    28381 gatcaaccac cagccgtagc gtttgtgcat ctttaggcgt tccggagcga atcgccttca
    28441 ccagattatt gccgctgaac aacaacggca gcccctgaat cacgcccgtt tgtttgatat
    28501 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    28561 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    28621 cacccacctg cgcgcacagc agcagcaacg tcgctaccaa ccaatttctg atgcgataca
    28681 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    28741 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    28801 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    28861 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcgaggcggt
    28921 acaaatcaaa gtgataaacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    28981 tggggctttt gacattaccc tgatggccca aagcctgcaa aaagccccgg ctaaaggtgg
    29041 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    29101 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    29161 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    29221 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    29281 gcgacgcagc ccgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    29341 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt cccggcatca
    29401 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    29461 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    29521 gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat cacgcgattg
    29581 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    29641 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg acccagaccg
    29701 ggaccaatca ccagcacatc ggcccattcc aggctttcgg taagagagtc catcgtcagt
    29761 tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc actgcgggtc
    29821 aatactcgaa ctaaaccagc accagcacgc agtgccgctt cccccgtcat acgaatagct
    29881 cccgccgtgc cgtgatcgcc gccaataatc accactctcc cgtgatcgcc tttatgcgat
    29941 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    30001 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtccggta
    30061 acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt gatggtgtga
    30121 tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc ggaagggata
    30181 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    30241 tcgcggggtg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    30301 tcgggccaga caatattcga agcatggatc tcgcctcccg cgtttaacca tgcttcgcgt
    30361 gccagcgcgg cctcttccgg caacggtttg tcactttcct gggccagcag cgtgacctca
    30421 ataccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    30481 ccgcacagca ccagccagtg gcgggcgtca ggataagccg tgcgacacac caggaatgcg
    30541 gcttcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcgcatc tgccgcctcg
    30601 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    30661 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaat
    30721 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    30781 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    30841 tggatggcgc gtcacggtat gctgcgcgct cgcccccatg agttattgcc cggtacgctg
    30901 cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    30961 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaagctt
    31021 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    31081 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagcagga
    31141 cttggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    31201 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    31261 ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    31321 gtcgatgctc gccgctgtat ctcttatctc accatcgaac tggagggggc gatcccggaa
    31381 gagttgcgac cgttaatggg aaatcgtatt tatggttgcg atgactgcca gcttatctgc
    31441 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    31501 cacgcaccgg aactcattga gttattcgcc tggagtgaag agaagttttt aaaagtcacg
    31561 gaaggttcgg cgattcgccg tataggtcac ctgcgttggc tgcgtaatat tgccgtagcc
    31621 ttaggcaatg cgccctggga tgaaacgatt ctggcggcgc tggaaagtcg taaaggtgag
    31681 cacccacttc ttgatgagca catagcgtgg gcgattgcgc agcaaatcga gagacgaaat
    31741 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt aattgaaaaa
    31801 gggttaccgc gtgatgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    31861 aattcaagac gaataaaatc gtcaagtgat cgact
//
LOCUS       DAYPHN010000038        31885 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000038 DAYPHN010000000
VERSION     DAYPHN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.038"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..747
                     /gene="ycaL"
                     /locus_tag="ACG0BW_001731"
     CDS             <1..747
                     /gene="ycaL"
                     /locus_tag="ACG0BW_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFK7258705.1"
                     /translation="LLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATLSDADAK
                     AIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            920..1603
                     /gene="cmk"
                     /locus_tag="ACG0BW_001732"
     CDS             920..1603
                     /gene="cmk"
                     /locus_tag="ACG0BW_001732"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFK7258706.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1714..3387
                     /gene="rpsA"
                     /locus_tag="ACG0BW_001733"
     CDS             1714..3387
                     /gene="rpsA"
                     /locus_tag="ACG0BW_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFK7258707.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3547..3831
                     /gene="ihfB"
                     /locus_tag="ACG0BW_001734"
     CDS             3547..3831
                     /gene="ihfB"
                     /locus_tag="ACG0BW_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFK7258708.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4038..6302
                     /locus_tag="ACG0BW_001735"
     CDS             4038..6302
                     /locus_tag="ACG0BW_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFK7258709.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIQWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYKWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6339..8087
                     /gene="msbA"
                     /locus_tag="ACG0BW_001736"
     CDS             6339..8087
                     /gene="msbA"
                     /locus_tag="ACG0BW_001736"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFK7258710.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8084..9070
                     /gene="lpxK"
                     /locus_tag="ACG0BW_001737"
     CDS             8084..9070
                     /gene="lpxK"
                     /locus_tag="ACG0BW_001737"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFK7258711.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVVDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGMRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            9107..10339
                     /gene="ycaQ"
                     /locus_tag="ACG0BW_001738"
     CDS             9107..10339
                     /gene="ycaQ"
                     /locus_tag="ACG0BW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFK7258712.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10391..10573
                     /gene="ycaR"
                     /locus_tag="ACG0BW_001739"
     CDS             10391..10573
                     /gene="ycaR"
                     /locus_tag="ACG0BW_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFK7258713.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10570..11316
                     /gene="kdsB"
                     /locus_tag="ACG0BW_001740"
     CDS             10570..11316
                     /gene="kdsB"
                     /locus_tag="ACG0BW_001740"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFK7258714.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11470..12363
                     /locus_tag="ACG0BW_001741"
     CDS             11470..12363
                     /locus_tag="ACG0BW_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFK7258715.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPIIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12340..13119)
                     /gene="elyC"
                     /locus_tag="ACG0BW_001742"
     CDS             complement(12340..13119)
                     /gene="elyC"
                     /locus_tag="ACG0BW_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFK7258716.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13255..14040
                     /gene="cmoM"
                     /locus_tag="ACG0BW_001743"
     CDS             13255..14040
                     /gene="cmoM"
                     /locus_tag="ACG0BW_001743"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFK7258717.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14037..15359
                     /gene="mukF"
                     /locus_tag="ACG0BW_001744"
     CDS             14037..15359
                     /gene="mukF"
                     /locus_tag="ACG0BW_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFK7258718.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15340..16044
                     /gene="mukE"
                     /locus_tag="ACG0BW_001745"
     CDS             15340..16044
                     /gene="mukE"
                     /locus_tag="ACG0BW_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFK7258719.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16044..20504
                     /gene="mukB"
                     /locus_tag="ACG0BW_001746"
     CDS             16044..20504
                     /gene="mukB"
                     /locus_tag="ACG0BW_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFK7258720.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAVAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20765..22612
                     /gene="ldtD"
                     /locus_tag="ACG0BW_001747"
     CDS             20765..22612
                     /gene="ldtD"
                     /locus_tag="ACG0BW_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFK7258721.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVAALREILQRTGMLDGGPKITLPGD
                     DTPTDTVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22793..23341
                     /locus_tag="ACG0BW_001748"
     CDS             22793..23341
                     /locus_tag="ACG0BW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFK7258722.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23368..24015
                     /gene="gloC"
                     /locus_tag="ACG0BW_001749"
     CDS             23368..24015
                     /gene="gloC"
                     /locus_tag="ACG0BW_001749"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFK7258723.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24066..25256)
                     /gene="aspC"
                     /locus_tag="ACG0BW_001750"
     CDS             complement(24066..25256)
                     /gene="aspC"
                     /locus_tag="ACG0BW_001750"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFK7258724.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25441..26529)
                     /gene="ompF"
                     /locus_tag="ACG0BW_001751"
     CDS             complement(25441..26529)
                     /gene="ompF"
                     /locus_tag="ACG0BW_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFK7258725.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(27132..28532)
                     /gene="asnS"
                     /locus_tag="ACG0BW_001752"
     CDS             complement(27132..28532)
                     /gene="asnS"
                     /locus_tag="ACG0BW_001752"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFK7258726.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(28701..29903)
                     /gene="pncB"
                     /locus_tag="ACG0BW_001753"
     CDS             complement(28701..29903)
                     /gene="pncB"
                     /locus_tag="ACG0BW_001753"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFK7258727.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            30169..>31885
                     /gene="pepN"
                     /locus_tag="ACG0BW_001754"
     CDS             30169..>31885
                     /gene="pepN"
                     /locus_tag="ACG0BW_001754"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFK7258728.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLDGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAT
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLL"
BASE COUNT         7815 a         7859 c         8571 g         7640 t
ORIGIN      
        1 ttgctggcga ttgcgacctc tgcagcctta ctgacaggat gtcagaatac gcacggtatt
       61 aataccgatt tggctatcag ctcaggtctg aatgcttata aagccgcaac attaagcgat
      121 gctgatgcta aagcaatcgc caatcagggc tgtgccgaaa tggacagcgg caatcaagtc
      181 gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa tcgccaaagc attgggtaac
      241 aatattaatg gcacgccggt caactataag gtttatatga ccagcgacgt caacgcatgg
      301 gcgatggcga acggctgtgt tcgtgtctac agtggcctga tggacatgat gaacgacaac
      361 gaaattgaag gcgttctggg tcatgaactg ggccacgtcg cgttgggtca ctcgctggct
      421 gaaatgaaag cttcttatgc gatcgttgcc gcacgtgatg cgatttccgc taccagcggt
      481 gtggcttccc agctttctcg ctcacaatta ggtgatatcg cagaaggcgc gatcaatgct
      541 aaatactccc gcgataaaga gtccgaagca gatgatttct cctttgatct gttgaagaaa
      601 cgtggcatca gcacccaggg gctggttggc agctttgaaa aattggccag cctggatggc
      661 ggtcgtaccc agtccatgtt tgactctcac ccaccatcaa cagagcgtgc gcaacacatc
      721 cgtgatcgta tcgcctctgg taagtaaatc attgtcatct ttcgggctgg tcttctgcca
      781 gcccgctata attgcgcaat aaatccccat ctgaatacag acaaaactgg tttttgcaca
      841 caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg cggttatgtt aacggtacgc
      901 ctgttttaag gagataaaga tgacggcaat tgccccggtt attaccattg atggcccaag
      961 cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa gcgttgcaat ggcatctgct
     1021 ggactcgggt gcaatttatc gcgtactggc actggcggca ttacatcacc atgttgatgt
     1081 tgcatcggaa gatgcgctgg tgccgctggc atcccatctg gatgtacgtt ttgtgtcgac
     1141 caatggcaat ctggaagtga tcctcgaagg ggaagatgtc agcggcgaaa ttcgtactca
     1201 ggaagtggcg aatgctgctt cacaagtcgc ggcattccca cgcgttcgtg aagcattatt
     1261 gcgtcgccaa cgcgcgtttc gcgaattacc aggcttgatt gccgatggcc gcgatatggg
     1321 aacggtggta ttccctgatg cgccagtgaa aattttcctt gacgcctcct cggaagaacg
     1381 tgcgcatcgc cgcatgctac agttgcagga gaagggcttt agtgttaact ttgagcgcct
     1441 tttggccgag atcaaagaac gcgacgaccg cgatcgtaac cgagcggtag cgccactggt
     1501 tccggcagcc gatgctttag tgttggattc caccacctta agcattgagc aagtgattga
     1561 aaaagcgcta caatacgcgc gccagaaatt ggctctcgca taagcgaccg aatttgcagt
     1621 acccccgttg caatggaatg acagcgggta tgttaaacaa ccccatccgg catggagcca
     1681 ggtggacgtt aaatataaac ctgaagatta aacatgactg aatcttttgc tcaactcttt
     1741 gaagagtcct taaaagaaat cgaaacccgc ccgggttcta tcgttcgtgg cgttgttgtt
     1801 gctatcgaca aagacgtagt actggttgac gctggtctga aatctgagtc cgccatcccg
     1861 gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc aggtaggtga cgaagttgac
     1921 gttgctctgg acgcagtaga agacggcttc ggtgaaactc tgctgtcccg tgaaaaagct
     1981 aaacgtcacg aagcctggat cacgctggaa aaagcttacg aagatgctga aactgttacc
     2041 ggtgttatca acggcaaagt taagggcggc ttcactgttg agctgaacgg tattcgtgcg
     2101 ttcctgccag gttctctggt agacgttcgt ccggtgcgtg acactctgca cctggaaggc
     2161 aaagagcttg aattcaaagt aatcaagctg gatcagaagc gcaacaacgt tgttgtttct
     2221 cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg atcagctgct ggaaaacctg
     2281 caggaaggca tggaagttaa aggtatcgtt aagaacctca ctgactacgg tgcattcgtt
     2341 gatctgggcg gcgttgacgg cctgctgcac atcactgaca tggcctggaa acgcgttaag
     2401 catccgagcg aaatcgtcaa cgttggcgat gaaatcactg ttaaagtgct gaagttcgac
     2461 cgcgaacgta cccgtgtatc cctgggcctg aaacagctgg gcgaagatcc gtgggtagct
     2521 atcgctaaac gttatccgga aggtaccaaa ctgactggtc gcgtgaccaa cctgaccgac
     2581 tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc tggtacacgt ttccgaaatg
     2641 gattggacca acaaaaacat ccacccgtcc aaagttgtta acgttggcga tgtagtggaa
     2701 gttatggttc tggatatcga cgaagaacgt cgtcgtatct ccctgggtct gaaacagtgc
     2761 aaagctaacc cgtggcagca gttcgcggaa acccacaaca agggtgaccg tgttgaaggt
     2821 aaaatcaagt ctatcactga cttcggtatc ttcatcggcc tggacggcgg catcgacggc
     2881 ctggttcacc tgtctgacat ctcctggaac gttgcaggcg aagaagcagt tcgtgaatac
     2941 aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg acgcagaacg tgaacgtatc
     3001 tccctgggcg ttaaacagct cgcagaagat ccgttcaaca actgggttgc tctgaacaag
     3061 aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg ctaaaggcgc aaccgtagaa
     3121 ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag catcccgtga ccgcgttgaa
     3181 gacgctaccc tggttctgag cgttggcgac gaagttgaag ctaaattcac cggcgttgat
     3241 cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag acgaagctga cgagaaagat
     3301 gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct ccaacaacgc aatggctgaa
     3361 gctttcaaag cagctaaagg cgagtaattc tctgactctt cgggattttt attccgaagt
     3421 ttgttgagtt tacttgacag attgcaggtt tcgtcctgta atcaagcact aagggcggct
     3481 acggccgccc ttaatcaatg cagcaacagc agccgcttaa tttgccttta aggaaccgga
     3541 ggaatcatga ccaagtcaga attgatagaa agacttgcca cccagcaatc gcacattccc
     3601 gccaagacgg ttgaagatgc agtaaaagag atgctggagc atatggcctc gactcttgcg
     3661 cagggcgagc gtattgaaat ccgcggtttc ggcagtttct ctttgcacta ccgcgcacca
     3721 cgtaccggac gtaatccgaa gactggcgat aaagtagaac tggaaggaaa atacgttcct
     3781 cactttaaac caggtaaaga actgcgcgat cgcgccaata tttacggtta agtttttact
     3841 caaacttgaa cgcgagaaaa gcacctgtcg ggtgcttttt tcatttctct aatctggaac
     3901 tggaagctgc ctcgcagagt tttgagcagt tttcaccctc tcgttaaatt cttctgaacg
     3961 tgcctcgggg aacgcaaaat tcccaccaga caaccgctca acaaagttgc acactttcca
     4021 taaacaggga ggggtgcatg aaaataacga cagtcggcgt atgcataatt tgcggaattt
     4081 ttccgttgct gattttgccc caattgcctg ggacattaac ccttgcgtct ctgaccgtct
     4141 tcgcctgcgt actggtattt atccctttta aaactgtccg ttatatcgcg ctgacgttgc
     4201 tgtttttcgt ttggggcata ttagcagcaa agcaaatttt gtgggcagga gaaaccttaa
     4261 ctggcgcgac gcaggatgca attgttgaga tcactgctac tgacggtatg accactcatt
     4321 acggtcaaat tactcatcta caaggtcgac gtatattccc tgcgccaggc ctcgtgctgt
     4381 atggcgaata tcttccgcaa gcggtttgtg ctggacaact gtggtcaatg aaactcaaag
     4441 ttcgtgcagt tcatggtcaa cttaatgatg gcggctttga tagccagcgt tatgccattg
     4501 cccagcatca gccgctcacc ggccgatttc tgcaggcaag tgtcattgaa ccgaattgta
     4561 gcctgcgtgc acagtatctg gcgtcattac aaacaacgct gcaaccctat atgtggaatg
     4621 cggttattct tggtttaggt atgggggaac ggttatccgt tcccaaagaa atcaaaaata
     4681 tcatgcgcga tactggaacg gcgcatttaa tggcgatatc gggattgcat atcgcttttg
     4741 cggcgttgct ggctgccgga ctcattcgcg gtgggcaagt ttttctgccc gggcgctgga
     4801 tccaatggca aatgccatta attggcggaa tctgctgtgc tgctttttat gcctggctga
     4861 ctgggatgca acctcctgca ttgcgtaccg tggtggcgct tgccatgtgg ggaatgctta
     4921 agttaagtgg gcgacaatgg agtggctggg atgtatggat atgttgtctg gcggcaattt
     4981 tgttgatgga tcctgttgcc attctctcgc aaagtttatg gctctctgcc gctgcggtcg
     5041 cggcactgat tttttggtat cagtggtttc cctgtccgga gtggcaactg ccgccggtat
     5101 tgcgcgcggt tgtttccctc atccatctgc agctgggaat caccctcctg cttatgcccg
     5161 tgcaaatcgt catatttcat ggcattagtc tgacctcgtt tattgcaaat ctatttgcaa
     5221 ttcccctggt gacatttatc acggttccat tgatcctcgc cgcgatggtt gtgcatttaa
     5281 gcgggccgtt aatcctggaa caggggttat ggtttcttgc cgaccggtct ttggctttac
     5341 ttttctgggg gttaaagagt ttgccggaag ggtggatcaa cattgctgaa cgttggcaat
     5401 ggctaacatt ttccccatgg ttcttactgg tggtatggcg attaaacgcc tggcgaacgt
     5461 tgccagcaat gtgtgtggct ggaggcttgc tgatgtgctg gccgctgtgg caaaaacctc
     5521 gacctgacga gtggcaagtg tacatgcttg atgtcgggca agggctggca atggtgatag
     5581 ccagaaacgg caaagcgatt ctctatgaca cgggactggc ctggcccgaa ggggatagtg
     5641 ggcaacaact gattatcccc tggctccact ggcataatct tgaaccggaa ggcgttattc
     5701 tgagtcatga acatctggat caccggggag ggctggactc aatattgcac acatggccga
     5761 tgttatggat cagaagtccg ttaaactggg aacatcatca gccctgtgtg cgtggcgaag
     5821 cgtggcaatg gcaaggattg cgtttcagcg cgcactggcc tttacaagct agcaacgata
     5881 aaggaaataa ccattcctgt gtggttaagg ttgatgacgg gacgaatagc attcttctaa
     5941 ccggtgatat tgaagccccc gctgaacaaa agatgctaag ccgttactgg cagcaagtgc
     6001 aggcaacatt gcttcaggta cctcaccatg gcagtaatac ctcatcatcg ttgccattaa
     6061 ttcagcgagt gaatggaaaa gtggcactcg catcggcatc gcgctataac gcatggcgat
     6121 tgccctccaa caaagttaag catcgctatc aacaacaagg ttataagtgg cttgatactc
     6181 cacatcaggg tcaagtgacg gtcaattttt cagcgcaagg ctggcggatt agcagcctca
     6241 gagagcaaat tttacctcgt tggtatcatc agtggtttgg cgtgccagtg gataacgggt
     6301 agaatatgcg gctatttcaa caaatgctgg ttttttgaat gcataacgac aaagatctct
     6361 ctacgtggca gacattccgc cgactgtggc caaccattgc gccttttaaa gcgggtctga
     6421 tcgtggcggg cgtagcgtta atcctcaacg cagccagcga taccttcatg ttatcgctcc
     6481 ttaagccact tcttgatgat ggctttggta aaacagatcg ctccgtgctg gtgtggatgc
     6541 cgctggtggt gatcgggctg atgattttac gtggtatcac cagctatgtc tccagctact
     6601 gtatctcctg ggtatcagga aaggtggtaa tgactatgcg tcgccgcctg tttggtcaca
     6661 tgatgggaat gcctgtttca ttttttgaca aacagtcaac gggtacgctg ttgtcacgta
     6721 tcacctacga ttccgaacag gttgcttctt cttcttccgg cgcactgatt actgttgtgc
     6781 gtgaaggtgc gtcgatcatc ggcctgttca tcatgatgtt ctattacagc tggcaactgt
     6841 cgatcatttt gattgtgctg gcaccgattg tttcgattgc gattcgcgtt gtatcgaagc
     6901 gttttcgtaa catcagtaaa aacatgcaga acaccatggg gcaggtgacc accagcgcag
     6961 aacaaatgct gaagggccac aaagaagtat tgattttcgg tggtcaggaa gtggaaacga
     7021 aacgcttcga taaagtcagt aaccgaatgc gtcttcaggg gatgaaaatg gtttcagcct
     7081 cttccatctc tgatccgatc attcagctga tcgcctcttt ggcgctggcg tttgttctgt
     7141 atgcggcgag cttcccaagt gttatggata acctgactgc cggtacgatt accgttgttt
     7201 tctcttcaat gattgcactg atgcgtccac tgaaatcgct gaccaacgtt aacgcccagt
     7261 tccagcgcgg tatggcggct tgtcagacgc tgtttaccat tctggacagt gagcaggaga
     7321 aagacgaagg taagcgcgtg atcgagcgtg cgactggcga cgtggaattc cgcaatgtca
     7381 cctttactta tccgggacgt gacgtacccg cattgcgtaa catcaacctg aaaattccgg
     7441 cagggaagac ggttgctctg gttggacgct ctggttcggg taaatcaacc atcgccagcc
     7501 tgatcacgcg tttttacgat attgatgaag gcgaaatcct gatggatggt cacgatctgc
     7561 gcgagtatac cctggcgtcg ttacgtaacc aggttgctct ggtgtcgcag aatgtccatt
     7621 tgtttaacga tactgttgct aacaacattg cttacgcacg gactgaacag tacagccgtg
     7681 agcaaattga agaagcggcg cgtatggcct acgccatgga cttcatcaat aagatggata
     7741 acggtctcga tacagtgatt ggtgaaaacg gcgtgctgct ctctggcggt cagcgtcagc
     7801 gtattgccat cgctcgagcc ttgttgcgtg atagcccgat tctgattctg gacgaagcta
     7861 cctcggcgct ggataccgaa tctgaacgtg cgattcaggc ggcattggat gagttgcaga
     7921 aaaaccgtac ctctctggtg attgcccacc gcttgtctac cattgaaaag gcagacgaaa
     7981 tcgtggtcgt cgaggatggt gtcattgtgg aacgcggtac gcataacgat ttgcttgagc
     8041 accgtggcgt ttacgcgcaa cttcacaaaa tgcagtttgg ccaatgatcg aaaaaatctg
     8101 gtctggtgaa tcccctttgt ggcggctatt gctgccactc tcctggttgt atggcctggt
     8161 gagtggcgcg atccgtcttt gctataaact aaaactgaag cgcgcctggc gtgcccccgt
     8221 accggttgtc gtggttggta atctcaccgc aggcggcaat ggaaaaaccc cggtcgttgt
     8281 ctggctggtg gaacagctgc aacagcgcgg tattcgcgtg ggggtagtat cgcggggata
     8341 tggtggtaag gctgaatctt atccgctgtt attgtcggca gataccacca cagcacaggc
     8401 gggtgatgaa cctgtgttga tttatcaacg cactggcgcg cccgttgcgg tttctcccgt
     8461 gcgttctgat gcggtaaaag ccattctggc gcaacaccct gatgtgcaga tcatcgtaac
     8521 cgacgacggt ttacagcatt accgtctggc gcgtgatgtg gaaattgtcg ttgttgatgg
     8581 tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg ccaatgcgtg agcgagcggg
     8641 gcgcttaaag tcggttgatg cggtaatcgt caacggcggt gtcccacgca gcggtgaaat
     8701 ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt accggtatgc gttgtgacgt
     8761 tgctcagctt gaacatgtag tggcgatggc ggggattgga catccgccgc gcttttttgc
     8821 cacgctgaag atgtgcggcg tacaaccgga aaaatgtgta ccgctggccg atcatcagtc
     8881 tttgaatcat gcggatgtca gcgcgttggt aagcgccggg caaacgctgg taatgactga
     8941 aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat tggtggtatt tgcctgtaga
     9001 cgcacagctt tcaggtgatg aaccagcgaa actgcttgcg caactaacct cgctggcttc
     9061 tggcaactag ttacgccgcg gcagcgttcg attgatggag tcatgaatgt cgctgccgca
     9121 cctctctctt gctgatgcgc gtaatcttca cctcgccgcg caaggcctgt taaacaaacc
     9181 ccgccgtcga gcgtcgttgg aggatattcc ggcaacgatc tcccgaatgt ccttgctgca
     9241 aatcgatacc attaatattg ttgcccgtag tccatatctg gtgcttttca gtcgtctggg
     9301 aaattatcct gcccagtggc tggatgagtc tctggcgcgt ggcgaattaa tggaatactg
     9361 ggcgcatgaa gcctgcttta tgccgcgtag cgactttcgt cttattcgcc accgcatgct
     9421 ggcacctgaa aaaatgggct ggaaatacaa agacgcctgg atgcaggaac atgaggcgga
     9481 aattgcacag ttaattcagc atattcatga taaggggccg gtacgttcag ccgattttga
     9541 gcatcctcgt aaaggtgcaa gcggctggtg ggaatggaag ccgcataaac ggcatctgga
     9601 aggtttattt actgccggaa aggtgatggt gattgaacgg cgcaacttcc agcgcgttta
     9661 tgatttaacc caccgagtca tgcctgactg ggatgatgag cgcgatctcg tttcgcaaac
     9721 agaagcagaa atcatcatgc tggataacag tgcgcgtagc ctgggaatat tccgcgaaca
     9781 gtggctggca gattactatc ggctgaaacg tccggcactg gcggcgtggc gcgaagcgag
     9841 ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa ttgggcaatc tttggctgca
     9901 tgctgatttg ctgccgctac tcgagcgtgc gctggccgga aagctcactg caacgcacag
     9961 cgcggtactt tcgccttttg atcctgttgt ctgggatcgc aaacgcgcag agcagctttt
    10021 tgattttagc taccggctgg agtgctatac cccagcgccg aaacgccagt atggctattt
    10081 tgttctgccg ttattacatc gtgggcaatt agttgggcga atggatgcca aaatgcatcg
    10141 ccagacaggc atccttgaag ttatctctct gtggttacag gaaggcatta aaccaacgac
    10201 aacgctgcaa aaagggttac gtcaggcgat tactgatttc gctaactggc agcaggcaac
    10261 gcgggtgaca ttaggacgct gcccgcaagg cctctttact gattgccgca ccggctggga
    10321 aatagacccc gtcgcataaa tgattatgat aagatttatg cattcataac ccgatccatc
    10381 tggaggaact atggatcatc gtctgcttga aatcattgcc tgcccggttt gcaacggaaa
    10441 actttggtat aaccaggaaa aacaagaact tatttgcaaa ctggacaacc tggctttccc
    10501 gctgcgcgat ggcattccag tgttgctgga aacggaagcc cgcgtgctga ctgctgatga
    10561 gagtaaatca tgagttttgt ggtcattatt cccgcgcgct acgcgtccac gcgtctgccc
    10621 ggtaaaccat tggttgatat taacggcaaa cccatgattg ttcatgttct tgaacgcgcg
    10681 cgcgaatcag gtgccgagcg catcatcgtg gcaaccgatc atgaggatgt tgctcgcgct
    10741 gttgaagccg ctggcggtga agtatgtatg acgcgcgccg atcatcagtc aggaacagaa
    10801 cgtctggcgg aagttgtcga aaaatgcgca ttcagcgacg acacggtgat cgttaatgtg
    10861 cagggggatg aaccgatgat ccctgcgaca atcattcgtc aggttgctga taatctcgcc
    10921 cagcgtcagg tgggtatggc gactctggcg gtgccaatcc acaatgcgga ggaagcgttt
    10981 aacccgaatg cggtgaaagt ggtcctcgac gctgaagggt atgcactgta cttctctcgc
    11041 gccaccattc cttgggatcg tgatcgtttt gcaaaaggcc ttgaaaccgt tggcgataac
    11101 ttcctgcgtc atcttggtat ttatggctac cgtgcaggct ttatccgtcg ttacgtcaac
    11161 tggcagccaa gtccgttaga acacatcgaa atgttagagc agcttcgtgt tctgtggtac
    11221 ggcgaaaaaa tccatgttgc tgtcgcccag gaagttcctg gcacaggtgt ggatacccct
    11281 gaagatctcg agcgcgttcg cgctgaaatg cgctaatttc acttcacgac actccagcca
    11341 attttgggag gagtgtcgta ccgttacgat tttcctcaat ttttcttttc aacaattgat
    11401 ctcattcagg tgacatcttt tatattggcg ctcattatga aagcagtagc ttttatgagg
    11461 gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt tactgggcga aaaactcagt
    11521 cgtattgagt gcgtcaatga aaaagcggat acggcgttgt gggctttgta tgacagccag
    11581 ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc ccggaaaagc ccgacaactg
    11641 gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc gtatgccgat tatttatggt
    11701 gtgatgacgc atgaagaaca ccctggcccc gatgtcctgc tactggagcg gatgcgtggt
    11761 gtttcggtgg aggcaccagc ccgaacacca gaacgctggg aacagcttaa agatcaaatc
    11821 gttgaagcct tactggcctg gcaccgtcag gacagtcgcg gttgcgtcgg cgcggtcgac
    11881 aatactcagg aaaatttctg gccctcatgg taccggcaac atgttgaagt gctatggacc
    11941 acgctcaatc agttcaataa caccggtcta acgatgcagg ataagcggat cctgtttcgt
    12001 actcgcgaat gtctcccggc attatttgaa ggctttaacg acaattgtgt gctgattcac
    12061 ggtaacttct gtttacgcag catgctgaaa gattcgcgca gcgatcagtt actggcgatg
    12121 gtcgggccgg gactaatgct ttgggcaccg cgagaatatg aactgttccg actaatggat
    12181 aattctctgg cggaagattt gctctggagt tacctgcaac gcgcgccagt ggcggagtcg
    12241 ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag ttgcgcaact ggttaatacc
    12301 ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat cactcttgcc gtggctcgcc
    12361 tgacgaacct tttagccact gccagatgcg gccaagcgtt tcatagccaa cacgatcgct
    12421 atgcatcaac caaaccggag aagggattgc ccgttcccac ggattaagcg gcgagtcgat
    12481 ggccaactga ttcgccgggg ctggcagcgg atttagccct tcctgctgaa aaaagatcat
    12541 cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa ggggcgtcac caatcgcctg
    12601 tttcactgcc gcagcttctt cttcggtatc ttttggcaaa tccagggtga taatttgctc
    12661 gcgcggcacg cccagcgatt gcgcaactcg ggcacctact tccgctgtac tcaccgtatt
    12721 ggtttttgct acgcctcccg tgaagatcag ttttgatccc ggattttcgc gccataagcg
    12781 aataccttca ttcaggcggg gcagactgtt attgattaaa ttcgagcttg gtgcccactg
    12841 cgggttccag gtataaccac ctccgagcac cacgatatag tccacttttt gcgaattatt
    12901 ccatgtcgga taggtgcttt cgatgggacg cagcaggcgg tcggctaccg gttgcaaact
    12961 caataacaag agcgccagcc acccaatgct aataaaaatc ttcccggttt tttgaaaacg
    13021 gctaaaccac agcagcgcca ggccagcgcc aataatcaac aacatcagcg gaagtggcag
    13081 caacatattg ccaatcactt ttttcagtgt aaaaagcatc ctttctggtt ccttttttaa
    13141 ccatatagca agggatcgcc agcgatatta caccagacgg gttcattctc gccgtggctg
    13201 tgacaaaata gcgtctttat cagaaaacgt cttagccaac cggtggagaa agcaatgcag
    13261 gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta acatttacgg caccaccaaa
    13321 gggcagcttc gacaggctat tctgtggcag gatctcgatc gcgtgctggc ggaaatgggc
    13381 ccgcaaaaac tgcgtgtgct ggatgctggc ggtggagaag ggcagaccgc aatcaaaatg
    13441 gctgagcgcg ggcatcaggt cattttatgc gatctttctg cgcagatgat cgaccgcgca
    13501 aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc aatttataca ttgcgccgct
    13561 caggatgttg cttcgcattt ggaaacgccc gttgatctga tattgttcca tgcggtgctc
    13621 gagtgggtgg ccgatccccg cagcgtatta cagaccctgt ggtcagtgtt gcgtccaggc
    13681 ggtgtgttgt cgttaatgtt ctacaatgcg cacggcttgc tgatgcataa catggtcgcc
    13741 gggaattttg attacgtgca ggcgggaatg ccgaaaaaga aaaaacggac gctttcgcca
    13801 gattatccac gcgacccggc gcaggtttat ctgtggctgg aagaagctgg ttggcaaatt
    13861 atgggtaaga caggcgttcg cgtgtttcat gattatctgc gcgagaagca ccagcagcgc
    13921 gactgctatg aagcattact tgaattagaa acgcgttatt gccgtcagga accgtatatt
    13981 accctggggc gttatattca tgtcaccgcg cgcaaaccgc agagcaagga taaagtatga
    14041 gtgaattttc ccagacagtc cccgaactgg ttgcctgggc cagaaaaaat gacttctcca
    14101 tctcgctgcc ggtagaccga ctctcttttc tgctggcggt tgccacgctg aacggcgagc
    14161 gtctggatgg tgagatgagt gaaggcgagc tggtggatgc attccgccat gtgagtgatg
    14221 cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa caacgcgatc aacgacatgg
    14281 tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc ggaagggaac gcaatttacc
    14341 gtctgacgcc gctcggcatt ggcatcactg actattacat tcgtcagcgc gagttttcta
    14401 cgctgcgcct ttctatgcaa ttgtcgattg tggcgggtga gctcaaacgc gcagcagatg
    14461 ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa tgtttatgcg ccactgaaat
    14521 attcggtagc agaaattttc gacagtatcg acctgacgca acgtctgatg gatgaacagc
    14581 agcagcaggt gaaggatgat atcgcccagt tgttgaacaa agactggcgg gcggcgattt
    14641 ccagctgtga attgttgctt tcagaaactt ccggcacgct gcgtgaattg caggatacgc
    14701 tggaagcggc aggcgataaa ttgcaggcca acctgttacg cattcaggat gcgacgatga
    14761 cccatgacga tctgcatttt gtcgatcgtc tggtattcga tctgcagagc aaacttgacc
    14821 gtattatcag ctggggccag caatccatcg atttgtggat tggctacgac cgccacgtac
    14881 acaaatttat tcgtactgcg atcgatatgg ataaaaaccg cgtctttgct cagcggttac
    14941 gtcagtcggt acaaacctat tttgatgagc cgtgggcgct aacttatgcc aatgccgatc
    15001 gtctgctgga tatgcgtgac gaagagatgg ctctgcgcga tgaagaagtg acaggagaac
    15061 ttcctgagga tctggagtac gaagagttta acgagatccg cgaacagctg gcggcgatca
    15121 ttgaagaaca acttgccgtg tacaaaacca gacaagtgcc gctggatctt ggtctggtgg
    15181 tacgcgaata tctgtcacag tatccgcgtg cacgtcactt tgacgttgcg cgcattgtta
    15241 ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt caccggactg ccagcgaaat
    15301 ggcagccgat taatgattac ggagccaagg tacaggcgca tgtcatcgac aaatattgaa
    15361 caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc cgttatttcc ggcgctggac
    15421 agcgccttac gttcaggccg ccatattggc ctcgacgaac tggataatca tgcattcctg
    15481 atggattttc aggaatatct ggaagagttt tacgcgcgtt ataacgttga gcttattcgt
    15541 gcaccggaag ggttcttcta tttacgccca cgttccacca ctcttatccc gcgttccgtc
    15601 ttgtcggaac tggatatgat ggtcgggaaa atcctctgtt atctctatct cagcccggaa
    15661 cggctggcga acgaggggat tttcacccag caggaactgt acgacgaact gctcaccctg
    15721 gccgatgaag cgaaactgct gaaactggtg aacaaccgtt caaccggttc agacgttgac
    15781 cgccagaagt tgcaggaaaa agtacgttct tcgctcaacc gtctgcgtcg tttgggtatg
    15841 gtgtggttta tgggccacga cagcagcaag ttccgcatta ccgaatcggt gttccgcttc
    15901 ggagccgatg tgcgtgctgg cgacgatccc cgtgaagcac agcgtcgcct gattcgtgat
    15961 ggcgaagcaa tgccgattga aaatcatctg caactcaacg atgaaaccga agagagtcag
    16021 ccagatagcg gagaggaaga ataatgattg aacgcggtaa atttcgctca ctgacgctga
    16081 ttaactggaa cggctttttt gcccgaactt ttgacctcga tgagctggtt acgacgctct
    16141 ccggcggtaa cggggcgggt aaatccacca caatggcggc gttcgttacg gcgctgatcc
    16201 ccgacctgac cctgctgcat ttccgtaaca ccacggaagc cggggccacc agcggttcgc
    16261 gcgataaagg tctgcacggt aagctgaaag cgggtgtctg ttattcgatg ctcgacacca
    16321 ttaactcgcg ccaccagcgt gtggtggtcg gtgtgcgtct gcaacaggtt gccggacgcg
    16381 atcgtaaagt cgatatcaaa ccgtttgcca ttcagggcct gccgatgtcg gtgctgccga
    16441 cgcagctggt gaccgaaacc ctcaacgaac gccaggcgcg tgtactgccg ctgaacgagc
    16501 tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa gcagttcaac tccattaccg
    16561 attaccactc gctgatgttc gatctgggca tcatcgcgcg tcgtctgcgc tcagcgtctg
    16621 accgtagcaa attctatcgt ctgatcgaag cttcgctgta cggcgggatc tccagcgcca
    16681 ttacgcgctc tctgcgcgac tacctgttgc cggaaaacag cggcgtgcgg aaagcgttcc
    16741 aggacatgga agcggcgctg cgcgaaaacc gcatgacgct ggaagcgatt cgtgtcaccc
    16801 agtcggaccg tgacctgttt aagcatctga tcagcgaagc caccaactac gtggcggcgg
    16861 actacatgcg tcacgccaac gagcgccgtg tccatctcga caaagccctg gaatttcgtc
    16921 gcgagctaca cacttcgcgt cagcaactgg cggctgagca gtacaaacac gtcgatatgg
    16981 cgcgtgagct ggcagagcac aacggtgccg aaggcgatct ggaagcagat tatcaggcgg
    17041 ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca gcaggagaag atcgaacgtt
    17101 acgaagcgga tctcgatgag ctgcagatcc gtctggaaga gcaaaacgaa gtggtggcag
    17161 aagccatcga acgccaggaa gagaacgagg ctcgtgcgga agctgccgaa ctggaagtgg
    17221 acgagctgaa aagccagctt gctgactatc agcaggcgct ggacgtccag caaacgcgcg
    17281 cgatccagta taaccaggcg gttgctgcgc ttaatcgtgc caaagaactg tgccatctgc
    17341 cggacttaac cgccgacagc gccgccgaat ggctggaaac cttccaggcg aaagagctgg
    17401 aagcgactga aaaaatgctc tctcttgagc agaaaatgag catggcgcaa accgcgcaca
    17461 gccagtttga gcaggcttat cagctggtgg tggcaatcaa cggcccgctg gcgcgtaacg
    17521 aggcgtggga tgtcgctcgc gaactgctgc gcgaaggggt cgatcagcgt cacctggcag
    17581 agcaggttca accgttgcgg atgcgcttaa gcgaactgga acagcgtctg cgcgagcagc
    17641 aagaagccga acgcctgctg gcagatttct gcaaacgtca gggcaagaat tttgatattg
    17701 atgaactgga agctctgcat caggagctgg aagcgcgcat tgcctctctt tccgatagcg
    17761 tgtctaacgc ccgcgaagag cgtatggcac tgcgtcagga gcaggaacag ctgcagtctc
    17821 gcattcagag tttgatgcag cgtgcgccgg tttggctggc tgcgcaaaac agtctcaacc
    17881 agttgagcga acagtgcggc gaagagttct cctccagcca ggacgtcacc gaatatctgc
    17941 aacagttgct ggagcgtgag cgcgaggcga ttgttgaacg cgatgaagta ggcgcgcgca
    18001 aaaacgccgt cgatgaagag atcgaacgtt taagccagcc aggtggttct gaagaccagc
    18061 gtttgaatgc gctggcggag cgttttggcg gcgtgctgct gtcagaaatt tatgacgacg
    18121 ttagcctgga agatgcgccg tacttctcgg cgctgtatgg cccgtcacgc cacgccattg
    18181 tggtgccaga tctgtcacag gtaaccgaac acctggaagg cttgaccgat tgcccggaag
    18241 atctctatct gatcgaaggg gatccgcagt cattcgatga cagcgtgttc agcgttgatg
    18301 agctggaaaa agcggtagtg gtgaaaatcg ccgatcgtca gtggcgttat tcacgtttcc
    18361 cggaagtgcc gctgtttgga cgcgcggcgc gcgaaagccg catcgaaagc ctccatgccg
    18421 agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt tgatgtacag aaaacccagc
    18481 gtctgcatca ggcgttcagc cgctttatcg gtagtcatct ggcggtggcg tttgagtctg
    18541 acccggaagc agaaatccgt caactgaaca gccgtcgcgt cgaactggag cgggcgctga
    18601 gtaatcatga aaatgataac cagcagcagc gtattcaatt tgagcaggcg aaagagggcg
    18661 ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt ggctgatgac agcctggcag
    18721 atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca ggaagccgcg cgttttgttc
    18781 agcagttcgg taatcaactg gcgaaactgg aaccgatcgt ttcggtattg cagagtgacc
    18841 cggaacagtt cgaacagtta aaagaagatt acgcgtactc tcagcagatg cagcgcgatg
    18901 cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg tcgtgcgcac tttagctact
    18961 ctgactctgc agaaatgctt agcggtaaca gcgatctcaa cgaaaaactg cgtgaacgtc
    19021 tggaacaggc ggaagcggag cgtacccgcg cgcgtgaagc gttgcgcgga cacgcagcgc
    19081 agttgagtca gtacaaccag gtgctggctt cgctgaaaag ttcttacgac accaaaaaag
    19141 agctactcaa tgatctgcaa cgtgaattgc aggatatcgg cgtgcgagct gatagcgggg
    19201 cagaagagcg ggcgcgtatt cgccgtgacg agctgcacgc gcaactgagc aataaccgtt
    19261 cacgccgcaa tcaactggaa aaagcgctta ccttctgcga agcagagatg gacaacctga
    19321 cccgcaaact gcgcaagctg gagcgggatt actttgagat gcgcgagcag gtagtgaccg
    19381 cgaaagcggg ctggtgtgca gtcatgcgca tggtgaaaga taacggcgtt gagcgccgct
    19441 tacaccgtcg tgagctggct tatctctccg ctgatgattt gcgttccatg tcggataagg
    19501 cgttaggtgc gctgcgtctg gcagtggcag ataacgaaca tctgcgcgat gtgctgcgca
    19561 tgtcggaaga tccaaaacgt ccggaacgta aaattcagtt cttcgtggcg gtttatcagc
    19621 atctgcgtga acgtattcgt caggatatta ttcgtaccga tgatccggtg gaagctatcg
    19681 aacagatgga gatcgaactt agccgtctga ccgaagagtt aacctcccgt gaacagaaac
    19741 tggcgatcag ttcccgcagc gtggcgaaca ttattcgcaa aaccattcag cgcgaacaga
    19801 accgtatccg tatgctcaac cagggtttgc agaacgtatc gtttggtcag gtgaacagcg
    19861 tacgtctgaa cgtgaacgtg cgtgaaacgc acgccatgct gctggatgtg ctctctgaac
    19921 agcacgagca gcatcaggat ctgtttaaca gcaaccgtct gaccttctcg gaagcgctgg
    19981 cgaaattgta tcaacgtctt aatccgcaga ttgatatggg gcagcgcacg ccgcagacca
    20041 ttggcgaaga actgctggat taccgcaact atctggaaat ggaagttgag gttaaccgtg
    20101 gttctgatgg ttggctgcgc gcagagtctg gtgcattgtc gaccggtgag gcgattggta
    20161 ccggtatgtc gattctggtg atggtggtac aaagctggga agatgaatct cgccgcctgc
    20221 gcggtaaaga tatctctcct tgccgcctgc tgttcctcga tgaagccgcg cgactggacg
    20281 cccgttctat cgccacgctg tttgaattgt gtgaacgttt gcaaatgcag ctcatcatcg
    20341 cagcgccgga aaatatcagt ccggaaaaag gcaccaccta taaactggta cgtaaagtct
    20401 tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg atttgcgccg caactccctg
    20461 aaacgcttcc aggaagtgac gaagcgcctt cccaggcgag ttaaaatgaa ggcggcagca
    20521 atgccgcctt ttctttttcc gaaaactccg tttctgcact aaaaaagtgg cacattacgg
    20581 cgcgcttttc tttaaacttc tttacattag gttatgtaaa aacgtatcgg cgtttatata
    20641 ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag tattggcgtt
    20701 gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag tatgataaaa acagggggca
    20761 agggatgttg cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc
    20821 cgtaacattc gctccactgt tcaatgcgca ggccgatgag cctgaagtaa tccctggcga
    20881 cagcccggtg gctgtcagtg aacagggcga ggcactgccg caggcgcaag ccacggcaat
    20941 aatggcgggg atccagccat tgcctgaagg tgcggcagaa aaagcccgca cgcaaatcga
    21001 atctcaatta cccgcaggtt acaagccggt ttatcttaac cagcttcaac tgttgtatgc
    21061 cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca
    21121 gctggcagag gtggcgattg ccggtttcca gccgcagttt aataaatggg tagagttact
    21181 gaccgatcct ggtgttaacg ggatggcacg cgacgtcgtg ctctctgatg cgatgatggg
    21241 ctatctccat ttcattgcaa atattccggt caaaggcact cgctggctat atagcagtaa
    21301 accttatgcg cttgcaacgc cgccgctttc ggtgattaac caatggcagc tggcgctgga
    21361 taaaggtcaa ttgccgacgt ttgttgcagg actggcaccg cagcatccgc aatatgcggc
    21421 gatgcatgaa tcgttactgg ccttactcag tgacaccaaa ccgtggcctc aactgaccgg
    21481 taaagcaacg ttgcgcccag ggcagtggag taacgacgta gcggcgttgc gcgaaatatt
    21541 gcaacgcaca ggcatgttgg acggggggcc gaaaattact ctacctggcg atgacacgcc
    21601 aactgacacg gtagtcagcc catccgctgt tactgttgaa acagcagaaa ctaagccgat
    21661 ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc gttcgtgccg cctacgataa
    21721 tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattgggag cagatggcgc
    21781 tattggcccg gcaacgcgtg actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt
    21841 ggcactcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg ggatcatggt
    21901 taacattcca gcctattcgc tggtctacta tcagaacggc aatcaggtac tggattcgcg
    21961 agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt
    22021 agtggtaaac ccgccgtgga acgtaccacc aactctggca cgcaaagata ttctgccgaa
    22081 agtgcgcaac gatccgggat acctcgaaag ccatggctat acggtgatgc gcggctggaa
    22141 cagcagagaa gcgattgacc catggcaggt tgactggtct acaatcacgg cctcgaattt
    22201 accgttccgc ttccagcagg ctccaggccc acggaactcg ctggggcgct ataaattcaa
    22261 tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc tgttcaagcg
    22321 tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg atctggcgaa
    22381 tatgctgttg caggatgcag gatggaatga caaacgtatt tctgatgcgc tgaagcaagg
    22441 cgatacgcgt tacgtcaata ttcggcagtc gattccggtg aatctctact acctgacggc
    22501 ctttgttggt gcagatggtc gtacccagta tcgtacagat atttacaatt atgatctacc
    22561 tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt aaatgaagca
    22621 gttcggggaa atcagttgtc gtaataatta gcatgatcgg gggcgattct ctgcagcccc
    22681 cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa ggtgcctctt
    22741 gtgcgccaga agtgcatata aacgataaca ttgacctgta gacttgatta tcatggacaa
    22801 attcgacgct aatcgccgca aactgctggc gcttggtggc gttgcactcg gtgccgccat
    22861 cctgccgact cctgcgtttg caacactctc taccccacgc ccgcgcattt tgacactcaa
    22921 taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag gctatattca
    22981 ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca aaataaagtc
    23041 catcgaccca ggattattcg atcagttgta tcgcctgcaa gggttattag gtacccgcaa
    23101 accggtgcaa ctcatttccg gttatcgttc cattgatacc aacaatgaac tacgcgcccg
    23161 cagccgtgga gtagcgaaga aaagctatca cacgaaaggg caggcgatgg atttccatat
    23221 tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg caggtggtgt
    23281 aggatattac ccacgtagta actttgtgca tattgatacc gggccagcac ggcactggta
    23341 gcaattgctt aacgaaacag gggcagtatg aactatcgta ttattccggt caccgcattc
    23401 tcccagaact gttcattgat ctggtgtgaa caaacccgct tggccgcact ggtcgatcct
    23461 ggcggcgatg cggaaaaaat caaacaggaa gttgacgcca gcggcctgac actgatgcag
    23521 atcctgctga cgcatggtca tctggaccac gttggtgcag cggcggaact ggcgcaacat
    23581 tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca aggcttgcct
    23641 gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc tgacgccgga tcgttggctg
    23701 aacgaaggcg ataccatcag catagggaat gtggctttac aggtgttaca ttgccctggg
    23761 catacgccgg gtcatgtcgt gttttttgat gatcgggcaa agctgctgat ttctggcgac
    23821 gttattttca aaggcggagt agggcgcagt gacttcccgc gtggcgatca taatcaactg
    23881 atttcttcaa tcaaagataa gttgctgcca ctgggggatg acgtgacatt tattccgggt
    23941 cacggaccat tatccacact tggttatgag cgtctgcata atcccttcct gcaagacgaa
    24001 atgcccgtct ggtaaaggcg cataaaaaag cccgcttttt aagcgggctt cattgttttt
    24061 aacgcttaca gcactgccac aatcgcttcg cacagcggag ccatgttatc tggtgtcatc
    24121 ccggctacgt tcacacgacc agaagcaaca gcatacacgc caaactcttc gcgcagacgc
    24181 agcacttgtt cttttgtcag accactgaag gagaacatgc cgttctgttt gatgataaag
    24241 ctgaagtcgc ggtttgcgcc tttttcctgc agcgtattga cgaacaactg acgcatacgc
    24301 tgaatacgct ggcgcatatc agtcagctct tgttcccaaa tcgcacgtaa cgcatcgttg
    24361 ctcaggatgg tggcaacaac agaagcgccg tgtgctggtg ggttagagta gttagcgcga
    24421 atcgccgctt tcatttggct gaatgcgcga tcaacggttt cactgtcagc agcaaccaga
    24481 gtacaagcgc caacacgctc gttgtacagg ccaaagtttt tagagtagga actggcaaca
    24541 atcagctctt tatgcatagc cgcgaaagcg cgcagtcctt cagcatcttc ttccagacca
    24601 cgggcaaacc cctggtaagc gaagtcaaac agcggtaacc agcctttctc aacggaaagt
    24661 tgtgccagtg tctgccattg ttccagcgta gggtcgatac cggttgggtt atggcagcag
    24721 ccatggaaca gcactacgtc gccagcctga gcttcgttca ggctgttaat cagtgcatcg
    24781 aagtcaaggg tgtgattttc cgcatcataa taagcgtatt cacgaacttc cagacctgca
    24841 gagttaaaga cgctcttatg gttcggccag cttgggttgc tcacccacac acgcttaacg
    24901 ctggtatttt ttgccaggaa atcggcagcc acgcgtagtg cgccagtgcc acccggagtc
    24961 tgtgccgtgc gagcacgttt gtcattgatc agggcgctac ctttaccaaa cagcagttcc
    25021 tgagtgcagc gaccaaattc agggatgccg tcaatgccga ggtaattttt ggtggtttca
    25081 ttttcgagca gatactgttc agcttttttc acgctggtca gtaccggggt tttgcccgtc
    25141 tcatctttat agacaccaat cccgaggtta attttgccgg gacgttcatc ggcacgaaac
    25201 agatcggcca ggcccagaat cgggtcggca ggagcggcgg taatgttctc aaacatgacg
    25261 aggttccatt atggttacag aagggaagtc cgctatcagg gtaacgggag atttacaaaa
    25321 ttccaactat tactgatgaa aacgcaggct gtttttgcaa gacgtgagat tgctctggaa
    25381 ggcataaaaa aaacaggacc aaagtcctgt tttttcggca tttaacaaag aggtgtgcta
    25441 ttagaactgg taaacgatac ccacagctac ggtgtcgtca gaacctacgc ccagtttgtt
    25501 gtcagaatcg atctggttga tgatgtagtc aacataggtg gacatgtttt tgttgaagta
    25561 gtaggttgcg cccacttcaa agtagttcac cagatcaaca tcaccgatac cttctacgtc
    25621 tttcgcttta gatttggtgt aagcgatgga cggacgcaga ccgaaatcga actggtattg
    25681 cgcaactaac agaacgtctt gcgttttgtt ggcgaagccg ctgatgtttg taaatttatt
    25741 agtgatcggc gtagcgttac gggtttcacc gtagttcgct gccaggtaga tgttgttcgc
    25801 gtcatacttc agaccagtag cccactgttc agctttttta ccgttgccaa gaggttgagc
    25861 ttcttgcagg ttggtacggt cagcggcgcc ataagcacca acgataccaa agccttcgta
    25921 ttcgtagctg atagaaccgc caacaccgtc gccgttagag cggcgtgcag tgtcacgctc
    25981 gtttttaccc aggtactgaa cagcgaagtt caggccatca accagaccaa agaagttgga
    26041 gttacgatag gtagcaacgc cgccaacacg accaacgaag aagtcatcgc tgtatgcagt
    26101 atcaccacca aattctggca gcatatcggt gtaacccagt gcgtcataaa ccacaccgta
    26161 gttacggccg taatcgaaag aaccaacgtc agcgtattta agacccgcga atgccagacg
    26221 cgttttgtta ccagtttgag cgtcagcgcc ttcagagttg ttaccctgga agttatattc
    26281 ccactgacca taaccggtca gatcggaatt gatttgagtt tcccctttaa aaccaagacg
    26341 ggcataggtc atgtcgccat tgccaccgta actgttttca ccgttaccct tggagaaata
    26401 atgcagaccg acagctttac cgtacagatc tactttgttg ccatctttgt tatagatttc
    26461 tgcagcgttt gcagtacctg ctactaacag agcagggacg atcactgcca gaatattgcg
    26521 cttcatcatt atttattacc ctcatggttt tttttatgac acctgccact gccgtcaata
    26581 agttctgtca ataaaaattt acggaactat tgatgagagt ttggtgtctt tatgtgtctg
    26641 caggcatctt tccattcaaa ctaacgtttc gctaccgtga aagtgctaca aagataaaga
    26701 tttggtttca aaaagaaaaa atatgtaact aaaagtaaaa attaaggaac tttgtgaaca
    26761 ccgtcatatt tccatagagg cgtgatgata tttacagcaa tttttatcta tttatatgat
    26821 ttccttatat ttaaattaac taaacggaaa ttttgtttct gatggaaact ttatcgacct
    26881 ggcacaaaat cctctttcag acatccagaa tgccaaaaaa tagtatgaaa attgtgctat
    26941 cagctaagaa aaaactaatc cgcaataaga atgcgggtta ttttttgtgc gtaaatgttg
    27001 caatgtaata ttccgtaaca ggatgatcgt tatggcgggg ttgcaaatag attgcttgac
    27061 ttaacaaaca gataacttga cagaaaggat aaaaaaagag ccagcgggtg ctggctcttg
    27121 aagacgctaa attagaagct ggcgttacgc ggagtacgtg ggaacggaat cacatcacgc
    27181 acgttttgca caccagttac gtaagcaatc aggcgttcaa aaccaagacc gaaaccggaa
    27241 tgcggaacag taccgtagcg acgcagatcg cgataccacc agtaatcttc tttattcagg
    27301 cccatttcca gcatacgctc gtccagcacg tccagacgtt cttcacgctg ggagccacca
    27361 atgatctcac cgatgcccgg agccagaacg tccatagccg caacggtttt accgtcttcg
    27421 ttaaggcgca tatagaacgc tttaatatct ttcggatagt ttttaaccac taccggtgct
    27481 ttaaagtgtt cttccgccag ataacgctca tgctcagaag agagatcaac gccccagtaa
    27541 actgggtttt caaacttcct gccgcagttt tcgagaatag tcactgcgtc ggtgtaatcc
    27601 acctgcgcaa aatcggcttc aatgaagcgt tccagacgtg aaacggcatc tttatctacg
    27661 cgttcagcga agaatttcat gtcgtcagcg cgttcttcga gaaccgcttt gaagacatat
    27721 ttcagcatgg cttcagccag acccgcgata tcgttcaggt tagcaaacgc cacttccggc
    27781 tccagcatcc agaattccgc caggtgacgg ctggtgttgg agttttcagc acggaaagtc
    27841 gggccgaagg tataaatttt ggacaatgcg caagcgtagg tttcgccgtt caactggcca
    27901 gatacggtca ggaaagactc tttaccaaag aagtctttgt cgaaatccac tttgccctga
    27961 tcgttacgcg gcaggttttc cagatccagc gtagaaacgc ggaacatttc gccagcccct
    28021 tcggtatcag acgcagtaat cagtggcgtt gaaacccaga agaatccctg ctcgttaaag
    28081 aagcgatgca gcgcctgcgc cagcgtatgg cgaacgcgcg cgacggcacc aatcaggttt
    28141 gtgcgcggac gcaggtgagc gacttcacgc agatactcaa tgctgtggcg ttttgccgcc
    28201 atcgggtaag tgtctggatc ttcaacccaa ccagcaactt caaccttgct ggcctgaatt
    28261 tcaaattgtt gcccctggcc cggcgatgcc acgactttac ccgtcacaat gaccgagcaa
    28321 ccggtggtca gacgcaggac gtcttcattg taattgggca gagaattatt gatgacagcc
    28381 tgtacaggat caaagcagga accgtcataa acggcgagga aggagatgcc agcttttgaa
    28441 tctcggcggg tacgtaccca tccgcgcacg gtgacttcgc tgtcaacggc tacgcggccc
    28501 tggagtacgt cggctacagg cacaacgctc ataatattct ctctgttaat agtcggaaaa
    28561 aataaacact tgtccacccg taaatggggg gatacctatg ttacctggca tctgcaatca
    28621 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
    28681 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
    28741 cgcagcgccc gaacaaacgc tttatcgtgg cagatagttt tgccagggct gtcagaaagt
    28801 ttcgccaccg gtttaccgtt acactctacc aacttaatga caatattcag gggttttacc
    28861 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
    28921 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
    28981 ttactctgtg gatcaattcc cagcttctca taatgtgcaa tggctttttc accccattca
    29041 accgggtcgc cggagtcatg acgcaggccc tgatagcgac tggcgaactc gacaccgaaa
    29101 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
    29161 tattcttcca gccaggcagc aagtgcagct cgctggctgt tggcaagatc cggactgatt
    29221 tgctgatgcg cctggaacca ttcgtgtgct tgtgttccca ttggcgtgag ggaaagccga
    29281 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgtttcacg
    29341 atggtttctt gtacttcacg agaaaaacgt cggcgggtgc caaaatccat cagatggaag
    29401 cgcgacatat caagaccggc ggttaatgcc gagaaatcgg ctaatttgct ttccagcgtg
    29461 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
    29521 accgccagca aaggaacttc ccagaggatg acttcacgcc acgggccgct taaacgaata
    29581 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
    29641 agccagttaa gatagtcggc cttaaagaaa ggcagggctg agagccattg atattcatca
    29701 tcctgcaggc gcaggtgctg catcgcctga acctgttcac gaatagcatc ggcataaata
    29761 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
    29821 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
    29881 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
    29941 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
    30001 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
    30061 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
    30121 aggaatagac tgaacaccag actctatcaa agatgctaaa ggttatttat gactcaacag
    30181 ccacaagcca aataccgtca cgattatcgt gcgccggatt accagattac tgatattgac
    30241 ttgacctttg acctggacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
    30301 catggtgcat cagatgctcc gcttcgtctt gatggcgaag atcttaaact ggtttctgtt
    30361 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    30421 aatttgccgg agcgttttac gcttaagatc gttaatgaaa ttagcccggc gacgaatacc
    30481 gcgctggaag ggctttatca gtcaggcgat gcgctttgca ctcagtgtga agccgaaggt
    30541 ttccgccata ttacgtatta tcttgaccgc ccggacgtac tggcgcgttt taccaccaaa
    30601 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    30661 ggcgaactgg aaaacggacg tcactgggta cagtggcagg acccgttccc gaaaccgtgc
    30721 tacctgtttg cgctggttgc aggcgacttc gacgtgctgc gcgacacctt caccacgcgt
    30781 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaacctcga ccgcgcgccg
    30841 tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt cggcctggag
    30901 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    30961 aataaaggtc tgaatatctt taactccaaa tatgtgctgg cccgtaccga caccgccacc
    31021 gataaagatt acctcgatat tgagcgtgtt atcggccatg aatatttcca taactggacc
    31081 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtg
    31141 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cggtaaaccg tatcaacaac
    31201 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccgatc
    31261 cgcccggata tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    31321 gcggaagtga ttcgcatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    31381 cagctctatt tcgagcgcca tgatggcagc gcggcaacct gcgacgactt tgtgcaggcg
    31441 atggaagatg cgtcgaatgt cgatctctct catttccgcc gttggtacag ccagtccggt
    31501 acgccggttg tgaccgtcaa agacgactat aatccggaaa ctgagcagta caccctgacc
    31561 atcagccagc gtacgcctgc tacgccggat caggcagaaa aacagccgct gcatattccg
    31621 tttgccatcg aactgtatga caacgaaggc aaagtgatcc cgttgcaaaa aggcggtcat
    31681 ccggtgaatt cggtgctgaa cgtcacccag gcggaacaga cctttgtctt tgataatgtc
    31741 tactttcagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    31801 aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgc
    31861 tgggatgcgg cgcaaagcct gctgg
//
LOCUS       DAYPHN010000039        31641 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000039 DAYPHN010000000
VERSION     DAYPHN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.039"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(197..325)
                     /gene="rhuM"
                     /locus_tag="ACG0BW_001755"
                     /pseudo
     CDS             complement(197..325)
                     /gene="rhuM"
                     /locus_tag="ACG0BW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(405..499)
                     /locus_tag="ACG0BW_001756"
     tRNA            complement(405..499)
                     /locus_tag="ACG0BW_001756"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Sec,seq:tca)
     gene            792..2174
                     /gene="yicJ"
                     /locus_tag="ACG0BW_001757"
     CDS             792..2174
                     /gene="yicJ"
                     /locus_tag="ACG0BW_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFK7258729.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2184..4502
                     /gene="yicI"
                     /locus_tag="ACG0BW_001758"
     CDS             2184..4502
                     /gene="yicI"
                     /locus_tag="ACG0BW_001758"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFK7258730.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGAEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKETVGQEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFHLFNLQDGHEALCEVPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(4555..6264)
                     /gene="yicH"
                     /locus_tag="ACG0BW_001759"
     CDS             complement(4555..6264)
                     /gene="yicH"
                     /locus_tag="ACG0BW_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFK7258731.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTESRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLLSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRNGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANIPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(6385..7776)
                     /gene="xanP"
                     /locus_tag="ACG0BW_001760"
     CDS             complement(6385..7776)
                     /gene="xanP"
                     /locus_tag="ACG0BW_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFK7258732.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8055..9260
                     /gene="gltS"
                     /locus_tag="ACG0BW_001761"
     CDS             8055..9260
                     /gene="gltS"
                     /locus_tag="ACG0BW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFK7258733.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9294..11375)
                     /gene="recG"
                     /locus_tag="ACG0BW_001762"
     CDS             complement(9294..11375)
                     /gene="recG"
                     /locus_tag="ACG0BW_001762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFK7258734.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRNDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11381..12070)
                     /gene="trmH"
                     /locus_tag="ACG0BW_001763"
     CDS             complement(11381..12070)
                     /gene="trmH"
                     /locus_tag="ACG0BW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFK7258735.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12077..14185)
                     /gene="spoT"
                     /locus_tag="ACG0BW_001764"
     CDS             complement(12077..14185)
                     /gene="spoT"
                     /locus_tag="ACG0BW_001764"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFK7258736.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14204..14479)
                     /gene="rpoZ"
                     /locus_tag="ACG0BW_001765"
     CDS             complement(14204..14479)
                     /gene="rpoZ"
                     /locus_tag="ACG0BW_001765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFK7258737.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14534..15157)
                     /gene="gmk"
                     /locus_tag="ACG0BW_001766"
     CDS             complement(14534..15157)
                     /gene="gmk"
                     /locus_tag="ACG0BW_001766"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFK7258738.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15415..17097
                     /gene="ligB"
                     /locus_tag="ACG0BW_001767"
     CDS             15415..17097
                     /gene="ligB"
                     /locus_tag="ACG0BW_001767"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFK7258739.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETPDVMMPPLNGAVIHPVAHTGVRK
                     MADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKTIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLLSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17094..17711)
                     /gene="yicG"
                     /locus_tag="ACG0BW_001768"
     CDS             complement(17094..17711)
                     /gene="yicG"
                     /locus_tag="ACG0BW_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFK7258740.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18002..18826)
                     /gene="dinD"
                     /locus_tag="ACG0BW_001769"
     CDS             complement(18002..18826)
                     /gene="dinD"
                     /locus_tag="ACG0BW_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFK7258741.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTSQVSIKQLENSVKITEKK"
     gene            complement(19048..19911)
                     /gene="yicC"
                     /locus_tag="ACG0BW_001770"
     CDS             complement(19048..19911)
                     /gene="yicC"
                     /locus_tag="ACG0BW_001770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFK7258742.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAITAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20038..20754
                     /gene="rph"
                     /locus_tag="ACG0BW_001771"
     CDS             20038..20754
                     /gene="rph"
                     /locus_tag="ACG0BW_001771"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFK7258743.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20820..21461
                     /gene="pyrE"
                     /locus_tag="ACG0BW_001772"
     CDS             20820..21461
                     /gene="pyrE"
                     /locus_tag="ACG0BW_001772"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFK7258744.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(21498..22094)
                     /gene="slmA"
                     /locus_tag="ACG0BW_001773"
     CDS             complement(21498..22094)
                     /gene="slmA"
                     /locus_tag="ACG0BW_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFK7258745.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22201..22659)
                     /gene="dut"
                     /locus_tag="ACG0BW_001774"
     CDS             complement(22201..22659)
                     /gene="dut"
                     /locus_tag="ACG0BW_001774"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFK7258746.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22637..23857)
                     /gene="coaBC"
                     /locus_tag="ACG0BW_001775"
     CDS             complement(22637..23857)
                     /gene="coaBC"
                     /locus_tag="ACG0BW_001775"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFK7258747.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAYFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24029..24697
                     /gene="radC"
                     /locus_tag="ACG0BW_001776"
     CDS             24029..24697
                     /gene="radC"
                     /locus_tag="ACG0BW_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFK7258748.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            24914..25150
                     /gene="rpmB"
                     /locus_tag="ACG0BW_001777"
     CDS             24914..25150
                     /gene="rpmB"
                     /locus_tag="ACG0BW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFK7258749.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25171..25338
                     /gene="rpmG"
                     /locus_tag="ACG0BW_001778"
     CDS             25171..25338
                     /gene="rpmG"
                     /locus_tag="ACG0BW_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFK7258750.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25436..26245
                     /gene="mutM"
                     /locus_tag="ACG0BW_001779"
     CDS             25436..26245
                     /gene="mutM"
                     /locus_tag="ACG0BW_001779"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFK7258751.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26284..26763)
                     /gene="coaD"
                     /locus_tag="ACG0BW_001780"
     CDS             complement(26284..26763)
                     /gene="coaD"
                     /locus_tag="ACG0BW_001780"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFK7258752.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26771..28048)
                     /gene="waaA"
                     /locus_tag="ACG0BW_001781"
     CDS             complement(26771..28048)
                     /gene="waaA"
                     /locus_tag="ACG0BW_001781"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFK7258753.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVSLGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            28461..29519
                     /gene="rfaQ"
                     /locus_tag="ACG0BW_001782"
     CDS             28461..29519
                     /gene="rfaQ"
                     /locus_tag="ACG0BW_001782"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFK7258754.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKAAEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQAAFWRNSFTHLVPLQGGNVVESNLSVLSPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLVCVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29516..30640
                     /gene="waaG"
                     /locus_tag="ACG0BW_001783"
     CDS             29516..30640
                     /gene="waaG"
                     /locus_tag="ACG0BW_001783"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFK7258755.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEVE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30633..31430
                     /gene="rfaP"
                     /locus_tag="ACG0BW_001784"
     CDS             30633..31430
                     /gene="rfaP"
                     /locus_tag="ACG0BW_001784"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFK7258756.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7500 a         7857 c         8443 g         7841 t
ORIGIN      
        1 aagttgattt gatatattaa atgaaaaaat taatcttgat gacagtctga ttattggtga
       61 agtttacagg tcagtttttt tcgccagctg ttaaaggaat agaatcgtcg tataaaaaag
      121 ggtgttatgg agcgtgtaaa acaatatctt caccttaatg gcgatacagt tggctgagag
      181 ccagcagggg gcaaatcacc tgcaaataaa cacggcacat gctggcgcgc atatcgcgga
      241 tgcgttcgag gacagaagaa ccgacaggcg gattcttcag tcgctcatcg tccatcacaa
      301 aacttttgat caggtattcc tgaagccgat tgaatattga gcagatagaa aatctctgga
      361 atgacagtat tacaaggggt tggagaatgt cagaagaggc taattggcgg aagatcacag
      421 gagtcgaacc tgcccgggac cgctggcggc cccaactgga tttgaagtcc agccgcctca
      481 ccggagacga cgatcttccg cgcctcgatt gctacatgga ggcggggcgc attatagcta
      541 cttccttgag tttctacatc ccccagatca gttttttccg cctaatacac gcagcgtatt
      601 atcacgaaag cattatagtt catataaaac ccatatttta caatgatata tgagcgaaat
      661 taacgctacc acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat
      721 tcgctctcac agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca
      781 aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg
      841 gagacgccgc cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata
      901 ccgatatttt tggcatacct gccggatttg tcggaaccat gtttttggtc gctcgtgcac
      961 tggatgcgat ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg
     1021 gtaaatttcg tccgtgggta ctgtttggcg cactgccatt tgggatcgtc tgtgtactgg
     1081 cctatagcac gccagatctc agtatgaacg gaaaaatgat ttatgcagca attacttaca
     1141 ccctacttac cttactttat accatcgtta atatccctta ctgcgcattg ggtggtgtaa
     1201 tcaccaatga cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg
     1261 cgggaggcat gctctctact gtgctgatga tgccgctggt taatttaatt ggtggtgata
     1321 ataaaccact cggtttccag ggcggtatcg cggtcctttc cgtggtggca tttatgatgc
     1381 tggcattttg tttcttcacc actaaagaac gcgttgaagc accacctaca acgacgtcta
     1441 tgcgggaaga tttacgtgat atctggcaaa acgaccagtg gcggattgtc ggtttactaa
     1501 ccattttcaa tatcctggcg gtgtgcgtac gcggtggagc gatgatgtat tacgtcacat
     1561 ggattttggg tacgccggaa gtgtttgtcg cttttctcac cacttattgc gtgggtaacc
     1621 tgattggttc cgcactggca aaaccgctga ccgactggaa atgtaaagtc actatcttct
     1681 ggtggacgaa cgccctgctg gcagtgatta gcctcgcgat gttctttgtt cctatgcagg
     1741 ccagcatcac catgtttgtc ttcatcttcg tgattggtgt gttgcatcaa ctagtgacac
     1801 ctatccagtg ggtaatgatg tccgataccg tcgactacgg cgagtggtgc aatggtaaac
     1861 gcctgaccgg gatcagtttt gctggcacgc tgtttgtgct caaactgggg ttggccttcg
     1921 gcggcgctct tatcggctgg atgctggctt atggcggata tgatgcggca gaaaaagcgc
     1981 agaacagcgc cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt
     2041 atttgctgag cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa
     2101 ccgttatgga acagctggcc cagggcaaac gccgttgcca gcaacaattc acctctcaag
     2161 aagtgcagaa ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg
     2221 gcctcaattt gattcacccg cttcaggtgt tcgaggttga acagcagggt aatgaaatgg
     2281 tggtctatgc tgccccccgt gatgtgcgtg aacgaacctg gcagcttgat acgcctttat
     2341 ttacgctgcg ctttttctcc ccacaggaag gtattgtcgg tgtacggatt gagcattttc
     2401 agggggcgct gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca
     2461 caatcgaaaa cacagaacgt tacgctgagt ttaaaagtgg caacttaagc gcgcgtgtca
     2521 gcaaaggtga gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc
     2581 aggtgaaaaa taatggctac gtgcaggaca cgaataatca acgaaattat atgtttgagc
     2641 ggctggatct tggcgttggc gaaacagttt acggtctggg tgagcgcttt actgccctgg
     2701 tgcgcaatgg ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg
     2761 cgtataaaaa tatcccgttc tatatgacta accgtggtta tggggtactg gtcaatcatc
     2821 ctcaatgcgt ctcttttgaa gtgggagcgg agaaagtctc caaagtgcag ttcagcgttg
     2881 agagtgaata tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc
     2941 gttatacccg ttttactggt cgtccggcgc tgccgcccgc gtggtccttc ggtctgtggc
     3001 taaccacttc atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta
     3061 tggcggaacg caatctgcca ctgcatgttt tccactttga ctgtttctgg atgaaagcct
     3121 tccagtggtg cgattttgag tgggacccgc tgactttccc ggacccggaa gggatgatcc
     3181 gccgcctgaa agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcgggcaaa
     3241 aatcccctgt ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt
     3301 cgctgtggca gtgggataaa tggcagccag gtctggcgat ttatgacttt accaatccgg
     3361 atgcctgcaa atggtacgcc gacaaactga aaggtctggt cgcgatgggc gttgattgct
     3421 ttaagactga ctttggcgaa cgtattccaa ccgatgttca atggtttgac ggttccgatc
     3481 cgcagaaaat gcataaccat tatgcgtaca tctacaacga actggtgtgg aacgtgctca
     3541 aggaaaccgt tggccaggaa gaagccgtct tgtttgctcg ctcggcctcc gttggtgcgc
     3601 agaaattccc ggtacactgg ggtggcgact gttacgctaa ctacgaatca atggcggaaa
     3661 gcctgcgcgg tggtttgtct attggccttt caggttttgg cttctggagc cacgatatcg
     3721 gcggctttga gaataccgct ccggcgcacg tttacaaacg ctggtgcgcg tttggtttgc
     3781 tctccagcca tagccgttta catggcagca aatcttatcg tgtgccgtgg gcctatgatg
     3841 atgagtcctg tgatgtggtg cgcttcttca cgcaactgaa atgccgcatg atgccgtatc
     3901 tgtatcgtga agctgctcgt gcgaacgcgc ggggtacgcc gatgatgcgg gccatgatga
     3961 tggagttccc ggacgatccg gcttgtgatt accttgaccg tcaatacatg ttaggcgaca
     4021 acgtgatggt tgctccggtg ttcactgaag cgggcgatgt gcagttctac ttgccggaag
     4081 gtcgctggac acatctgtgg cacaacgatg aactcgatgg tagtcgctgg cataaacagc
     4141 agcacagctt cctgagtctg cccgtttatg tgcgtgataa caccctactg gcgctgggca
     4201 acaacgagca acgtcccgat tacgcgtggc acgaaggcac ggcattccat ctctttaatc
     4261 tgcaagacgg gcatgaagct ctttgtgaag tgcccgctgc tgacggttcg gtgatcttta
     4321 ctctaaaagc aacacgtact ggtaacacga ttactgtgac tggtgcgggc gaggcgaaga
     4381 actggacact gtgcttgcgc aatgttgtga aagtaaatgg tctgcaaggc ggttcgcagg
     4441 ctgaaagtga gcaggggctg gtggtgacgc ctcaagggaa tgcgctgaca attacgttgt
     4501 aatctattga cggaatgccg ggtacgtctg agtcgtaccc ggcgagattg atttttactg
     4561 caccggctcc gtcgacgtaa cttcaccact ggaaacgacg cccgcattca tggtttgagt
     4621 cacttgctgc tttgcggcgt tcacggcatg gagttgcccg ctaaccgtag gtttcagcgg
     4681 gatattggcc tgaatatcgc cgctggcggt aagctgaata ttgccgtcgc cagtcaacgg
     4741 taattcaggc catccccact gttgcaaaat attcaccggc acaccgcgtc cattcaggct
     4801 gatatgggtc tgacgttgtg gcgtttgtga aacactggcg gtggcttcca gaatgccttt
     4861 ttcagtaaat gcactcagtt cgctgatatt caccgtgctg ctgttggcgg tcagcgccag
     4921 cgatgggcga cgaacatcaa cacgattgaa tgtcgcggcg gcggcattca gattcgccga
     4981 accactccag acgccccatt tatgatcggt aaccagcgtc aggttggcac cgtaaccatc
     5041 gagcgcggtg agctgccacg ggaagtcagg gtcgatatca atgatcagat tgcggctggc
     5101 gctaaatctc ttcagttgca ggctgtttaa ccaacccggt gtcgtttcca tccacaactg
     5161 ttgccagttt tttggcaagg tatattccag tccggcaatt gccgcatcat caaggatcaa
     5221 cgttttcccg ttgcgcagcc agtttccgga cgttctgacc atgccccctt cccagcggct
     5281 ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc gcgtttataa tcgggtcaaa
     5341 taaatgcagc gaaccataaa tgaactcgct agcgttcatc gacagtttgc cttcctgtgt
     5401 ctgccagtca tctttactga aggtcatgtt gcgcaagctg agatcgaggt cggtcaccgc
     5461 ccagtccgga ccttgcaggc gagcgtcgat gacttccagg cgaccaatct gcaacgacgg
     5521 gacagagagt aatggcgcaa agaagtcggt tagcgatttt tcgctttgta gacggatatc
     5581 cgccatgcgc agattttcca cttgccagct gccgtcggcg ttacgctgcg cgtttccggt
     5641 taatgtcccg cgggcgatgt cggcacccag gttagtcagc gtaacgcgat cgttatcaat
     5701 actgccttca atcagtacat tggtggcagg aacatcattg agcgaaagcg atccggcact
     5761 aaactgaatc tgcgccttcg tacccagcac tttaccggct tctggtgacc acggaaccac
     5821 gccgccattt acccgctgcg cgctcagttt ccattcgcta ttcgggctat taaacgccat
     5881 atcacgcagt tgcagacgat cggctttgaa cggtagcggc gcggtctggt cggtgagatt
     5941 cagcgtcccg ttttccagca ggatggtatc gacatggcgt gattcggtca gttggcgact
     6001 gcttagcgca atgtcgacac ttttggccac cagggtcgcg ggctgaccat cacgaccaaa
     6061 cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa cggtgatcca tcgccccgaa
     6121 ggccagatga tagtcactat tttcggaaac ccatgcgctg atatgttctg ctccccagcg
     6181 ggtttgcaga agaaaataga ggccagcgat caccactaac agagcgatga gaatgtagag
     6241 aagcagcttc ccaataaatt tcatggtctt ccatcccgtg aaatgcacat aagggagtta
     6301 tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga ctgcggcggt
     6361 aattatctta ccgccgccag tgaattactg tttttctggt gggaaaatca gattcagcac
     6421 gatggcagta ataccgcccg cggcgatccc ggaggagagc aggtttttca gccattcagg
     6481 ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga ccgaccgcca gcgacagcgc
     6541 gataatcaga atcgcccgac ggttcagcgg ctcacgagac acaatgcgca caccggaggc
     6601 ggcgatggtg ccaaacatta caagcgttgc gccgcccaga accggttctg gaatatgttg
     6661 tacaaaaccg ctcaccgccg ggaagagacc cagcacgatc aacatcagcg cgacgacaaa
     6721 accgacatag cggctggcaa caccggtcaa ctggatcaca ccgttgttct gcccgaagca
     6781 ggagttcggg aaggtgttaa ataccgccga aacaaacgag ttcaggccgt ttgccagcac
     6841 gccgcctttc aggcgtttca tgtacagcgg accggacact ggctgttcgg aaacgtcaga
     6901 ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc ataaagacca gcatcagcgg
     6961 cagcagcaga ctccattcaa tgccaaggcc gtaatagagc ggcgttggta ccataatcag
     7021 ttcttgcgtc atcggttcgt tgctttctgg caacatgccc ataaaccacg ccagcgcata
     7081 tccggccgcc atcgcaatca ccagtgaggc cacgcgcaaa tatgggttac gttgacggtt
     7141 aagcaggata attaaggcta agaccacgcc tgccagcaac agatttttcg gtgcgccgaa
     7201 ggtgttatcg ctcatggctg cgtaaccgcc gccaatggat gttagcccca cctgaattag
     7261 cgacaggccg ataatcatca ccacaacgcc agaaaccagc ggcgtaataa tgcggcgcgc
     7321 cagatgcaga acacgggaga tcaccatctc ggtgcaactt gccagcatca acgtgccgaa
     7381 caaagccgcc atcatggtag gaacatcagc accaccggtt ttcagcgcgg taccgcccat
     7441 aatcagcggg gcaacaaagt tgaagctggt gccctgaata gacaacagcc cggagccaac
     7501 cggaccccag gccttaattt gaataatcga tgccacaccg gaggcaaaca gcgacatact
     7561 aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta atagcgctgg
     7621 cgtgatcacc gcaacgaaca tcgccagcag atgctgacag gcggcaaaca gagtttgagg
     7681 aagcggcgga cgatcttcaa gacggtaaat cagttcgctg ttttgagtct gcgcaaccgg
     7741 ttgcgcattt tctgactcga gggtggaaac agacatcgaa aacaatcccg tggtagcaaa
     7801 gcgggcattt tagctgaccg aaagataaaa gcaaacgttt gccacatcct catctaaata
     7861 tttaaatgag ataaatgcac ttttttatat aacttttggt atttttctgc ttaaaatcca
     7921 tgccatttgt tcattaacag ctgagaattt gacccaggaa agcataactc gatacccgtc
     7981 atagttcata agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat
     8041 acaaaggagt aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga
     8101 cgttgctgct cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac
     8161 cggaaccagt tgcgggtggt ttgttggtgg cgctggcgct actggtactg aaaaaaagca
     8221 tgggctggga agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gcattcttcg
     8281 ccaccattgg cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca
     8341 tcttcttgat tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg
     8401 ctagcctgtt agggcttgat ccgctgatgg ggctgctggc cggttctatt acgctttccg
     8461 gcggtcacgg tacgggagca gcatggagta aattgttcat tgaacgttat ggcttcacca
     8521 atgcgacgga agtggcgatg gcctgtgcga cgtttggtct ggtgctgggc ggcttgattg
     8581 gcggtccggt agcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg
     8641 accaggaagt cccgacggcg ttcgaaaagc cggatgtggg ccgcatgatc acctcgctgg
     8701 tgctgattga aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc
     8761 aacttttggc tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg
     8821 tgattctgag caacggtctg tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat
     8881 ccgtgcttgg taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac
     8941 tatgggagct ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct
     9001 tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg
     9061 cagtgctggc tgcgggtcat tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca
     9121 acatgcaggc gatcactgaa cgttttggcc cgtcgcacat ggcgttcctg gtagtgccga
     9181 tggtcggtgc gttctttatt gatatcgtta acgcgctggt gattaagctg tatttaatgt
     9241 tgccgatttt tgccggttaa ccgatgaagc ggcggtcttc tcaccgccgc cttttacgca
     9301 ttcgagtaac gttccgtctc cggcatccag cgttctatca gggcttttgc ctgttgtggg
     9361 taacgttcgt gaatatgtcg cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc
     9421 agtaaatccg ccactttaaa ctcagcatta cccgtctgac gcgtacctaa cagttcaccc
     9481 ggtccgcgaa tctctaagtc tttctgtgca atcacaaaac cgtcgttgct gtcgcgcagt
     9541 acctgcaggc gaatttgcgc cgttttagag agcggtgttt tgtagagcaa cacgcagtga
     9601 gaagccaccg caccacgacc tacacgcccg cgaagctggt gcaactgcgc cagacccaga
     9661 cgctccgggt tttcgataat catcaggctg gcgttaggca catcaacgcc gacttcaata
     9721 acggtagtgg caaccagcag gtgtaactcg ccttgtttaa atgatgccat gacggcctgc
     9781 ttctcggctg gtttcatccg tccgtgtacc agaccaacat tcaactctgg tagcgccagt
     9841 ttcaactctt cccaggtagc ttccgccgcc tgcgcttcca gtaactctga ctcttcaatg
     9901 agtgtgcaca cccagtacgc ctgacgccct tcagtcatac aggcgtggcg cacgcggtcg
     9961 ataatgtcgt tacgacgcgt atccgggatt gccactgtcg tcactggcgt gcggcctggc
    10021 ggcagctcat ctatcaccga ggtatcgaga tcggcatacg cggtcatcgc cagcgtgcgg
    10081 gggatcggcg tcgcggtcat gatcaactga tgcggatgga agccctgctg ctggcctttc
    10141 tcccataacg ccagacgctg atgcacgcca aaacgatgct gttcgtcgat aatcaccagc
    10201 gccaggccgt taaactgcac ctgttcctgg aagatggcgt gtgttccgac aatcatctgc
    10261 acctgaccgc tggcgatggc ttcctgctgt gccagccgtg ctttaccttt ctgtttaccg
    10321 gcgagccagc ccacttcgat accgagcggt gcaaaccagt tgcggaagtt attggcgtgc
    10381 tgttcggcga gtaattcggt tggtgccatc aatgccacct gtttgccgtg ggcaatcgca
    10441 cgcagcgcgg cgagggcagc gaccagcgtt ttaccggaac ctacatcgcc ctgtaccagg
    10501 cgcatcatcg gcgcatccag cgccatatcg cgctcgatct ccgccaccac gcgtgcctgt
    10561 gcgcccgttg gcttgaacgg taaggctgcg aggagtttat ttttcagcgc gtcattggcg
    10621 ctaagcggct gggcatggaa acgttgtgca ccagcgcgta acgccaacat gctgaggttg
    10681 tgcgcaagca gttcttccag aatcagacgg cgttgtgctg gatgctgccc ggtttcgaga
    10741 tcgctaagct gtagcgtcgg cggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc
    10801 atcattcctt gtgacagttc cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga
    10861 tccagcgcct gatcggttaa tttacgcagc gtggcctgct ttacgccttc cgtagttggg
    10921 taaaccggcg tgagcgtttc ctgtaattct ggcgtgctga gatcgccctg cacgcggtat
    10981 tccgggtgga tcatctccgc accatattta ccgcgctttg cttcgccata agccagtaca
    11041 cggcggcccg tcgccaggct atttttcatt gccgcgttga agttgaaaaa gcgcatggtg
    11101 agaatgccgg aaccgtcgct gatctggcag gtcatcatcc gccgaccgcc gaaggagata
    11161 ttgcaattca gtacttcgcc ttctaccgtg gcataaacgc ccggtagtag ttctccgatg
    11221 gggtagagat gggtgcgatc ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg
    11281 gtatgcaggt tgattttcgc cagcttgtta ctaagcgctg caccaacgcc cgttagggaa
    11341 ctgagcggga cagcatctaa caggcgacct ttcatggtac ttaccctgca gcttgcatag
    11401 tggcccacca gtcggcatcg gcttcaattt cgccctgctg attgacgtgg gggtaaggca
    11461 ggcctttgcg ttttgcgact ttcgccagca ccggatagcc gccttcaaac agcaggcgtt
    11521 gttgctctgc ttccggcaac atgctgtttt cacgcaggta catgcccgca ttttgccgct
    11581 ggcgctgggc ttcgtaaaga atgagggctg aggcaacgga aacattcagc gattgcacca
    11641 tgccaatcat cggaatgatg atgtcccgat ccgccagggc caacgcttcc tgcgtgatgc
    11701 ctgttttctc ctgtcccatc aaaatacagg tcgggcgagt gtaatcaatt tcgcggaaat
    11761 cgacagcgtt atcagaaaga tgggttgcca gaatctgcat gccctggcct ttcagatgag
    11821 cgacggcatc gccaatggtg cggtgcgttt tcacctgtac ccagctgtta ctacccgcag
    11881 ctgccgaagc catggtgcgc atccgactgc caggccagac agcatgaact tcatgtacgc
    11941 caacggcatc tgcagtacga ataatcgcag aaacgttatg aggtttgtgg acctgctcca
    12001 tgcagacggt cagatcaggc tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg
    12061 ttgggttcat aaaacattaa tttcggtttc gggtgacttt aatcacgtct ggcatcacgc
    12121 ggattttgcg catgatattc gccagatgca cacggtcacg ggcggtcaga cgaataaagg
    12181 cgctgtagac gcgaccatct ttctcttccg tattcaaact ttgaatattc gaggtggtgg
    12241 tgttaattgc cgctgtcagg tttgccagcg cgccctgatg attgaacatc tccaccttga
    12301 tttcggtgat gaactcctgc gccgtctctt tatcccattc cacagccata aacttctctg
    12361 gctctttctg gtagccacgg atgttacggc aggattcatg gtggatcacc agacctttac
    12421 cggggctgac gtgggcgata atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg
    12481 tgatcagcac gccatcggca cctttaatgg gcagatgtcc gtgactttgg gttgccggtg
    12541 gaatggaggc gtccccatgt tgcagatttt tcgcgaccac cacgctcatt gcgttaccaa
    12601 ggccgatttc agccagcaga tcgtcaagcg tagccagctt catgcgatcc agctcgcgct
    12661 gaatattttc ctgcgggatt tcattgagct tacggctacc ccccaacgca tggttgagca
    12721 gacgacggcc cagactaaca gaatcatcac gcttgaggtt tttcagcaac tgacgaattt
    12781 ttgcgcgcgc tttcgagctg acgacaaagt tcagccacgc ggcattcggg cgagcgcccg
    12841 gtgcggtaat gatttcaacg gtttgaccgc tggtaagcgg ctgcgacagc gggtaaggct
    12901 gacggtcgac acgcgcgccc acgcaggcat gaccgatatc ggtatgcacc gcataagcga
    12961 agtcgacggg cgttgcaccg gcaggcagct ctacaatgcg cccttccggt gtgaaaacgt
    13021 aaatctcatc cgggaagaga tcggatttaa cgctctcgat aaattcaaac gagctaccgg
    13081 cactctgttg cagctccagc aggctttgca tccagcgctg ggcgcggatt tgtgcggtgg
    13141 tgctggtttc gccgtgctct ttataagccc agtgcgcggc aacacccatc tctgccatct
    13201 gatccatatc ttcggtacgg atctggacct caaccggcac gccgtgcggg ccaatcatcg
    13261 aggtgtgcaa agactgatag ccgttcgctt ttggaatggc gatgtagtct ttcacgcgac
    13321 ccggacgcgg cttgtacagg ctatgcattt gcccgagcac gcgataacag gtgtcggaat
    13381 cattgacgat cacgcggaaa gcgtagatgt ccatgattga gtgaaaacgc tgctctttga
    13441 gcaccatttt gcagtagatc gaataaagat gcttttcgcg accactgacg cggcacggta
    13501 ttcccgcttc ctgcaaacgc ccttcgattt cagaaagaat cttctggatc atctctttac
    13561 ggttgccgcg cgcggctttc accacttctt tgattacgcg ataacggttg ggatacagcg
    13621 cctcaaaacc cagctcttcg agttcggttt taatgtggtg gatacctaaa cggtgcgcca
    13681 gcgggctgta aatttcgagt gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg
    13741 agcccagcgt gcgcatgttg tgggtacggt cggcaagttt gatgagaatg acgcggatat
    13801 cctgcaccat cgccataatc atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc
    13861 ggaacttgag cttatcaagt ttcgacaccc cctctaccag ctcggcgacg cttttaccaa
    13921 aaagctgttc catgtcctgg taggtggcgg gtgtgtcttc aatcacgtca tgcagcagcg
    13981 ccgccatcag cgtttcatag tcgagtttca tctcggccag aatgcaggca accgccaccg
    14041 ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa
    14101 cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt
    14161 gattcaggct ttcaaacaga tacaagggcg acccgctttg tgattaacga cgaccttcag
    14221 caatagcggt aacggcttgt aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc
    14281 gaacgtcgag gatctggttg ttgatcagac cttcttcgat ttcgcgcagc gcgattacag
    14341 tggttttatc gttttcttcc ggtaccagcg gatcctttcc gcctacctgc atctgacgag
    14401 cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc aattttctct acagcgtcct
    14461 gaacagttac gcgtgccata cttaaaaagc tccacaggtg aagaaatgac tgggcatgat
    14521 actgaaatca ggttcagtct gccaacaatt tgctgattaa agcgtcatga cgctgctttt
    14581 ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt caaatcggtc aacgcagtat
    14641 cgaagtcatc attcacaatc aggtaatcat attcggcgta atggctcatt tctgcaacag
    14701 cttgcgccat acgctttgca atgacctctt cgctgtcctg accgcgaccg cgtagacggc
    14761 ggtccagttc aattttggac ggcggtaaaa taaagatact ccgcgcgtgc ggcatcttct
    14821 ggcgaatttg ctgcgcgccc tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca
    14881 ggacttgctc aatggcctca cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt
    14941 cgaggaacgc atctctgcta atcatttctt taaattcatc atgattaaca aagaaataat
    15001 gttcaccgtg gacttcacca ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg
    15061 tgtcatacaa cggttgggtt tttaataaag cctgaatcag gctggattta cccgcgccac
    15121 tgggggcaga aacaatataa agcgtgcctt gagccatgag tatctttcgt atgtgattag
    15181 cgaaataaag cctacatacg agcttattat acacggcgcg gccatgtgac gtagcccttg
    15241 ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct gcttccgcat ttgcctgtca
    15301 ttcactgcaa taaagacgat tctcaggaag cgtcctcgca gactccgttt ttgccactcg
    15361 gctttgcttt catcacgttg tatatctgcc tgacaatcta cagcggaggt gatgatgaaa
    15421 gtatggatgg cgatattaat aagtatcttg tgctggcaat catcggcgtg ggcggtctgt
    15481 ccggcctggt cgccagccag agcacaggaa gaaatttccc gcctgcaaca gcaaataaaa
    15541 cagtgggacg atgactactg gaaggaaggg aaaagtgagg ttgaagacgg tgtttacgat
    15601 cagttaagcg cccgtcttac gcagtggcaa cgctgttttg ggaacgagac ccccgatgtg
    15661 atgatgccgc ctttaaacgg tgcggttata catccggtag cccatacggg tgtacgcaaa
    15721 atggcggata aaatcgcatt aagtctgtgg atgcgcgaac gtagcgatct ttgggtgcag
    15781 ccaaaagttg atggcgttgc ggtaaccctg gtttatcggg acgggaaact gaacaaagca
    15841 atcagtcgcg gcaatggcct gaaaggcgag gactggacgc agaaagttcg cttaatttcc
    15901 gctgtgccgc aaaccgttag cgggccttta gccaacagta cgcttcaggg ggaaatattt
    15961 ctcaaacgcg aggggcatat ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt
    16021 gctggtctga tgatgcgcca ggacgatagc gacacgctga attctctagg cgtttttgtc
    16081 tgggcatggc cggatggacc gcagttaatg accgatcgtt taaaagagct ggctaccgct
    16141 ggttttactc taacgcagac gtatacccgt gcggtgaaaa atgctgatga agttgcgcgc
    16201 gtacgcaatg cgtggtggaa agcgaaatta cccttcgtca ccgatggcgt ggttgtgcga
    16261 gcggcgaaag aacccgaatc ccgccattgg ctaccaggcc aggcagaatg gttggtggcc
    16321 tggaaatatc aacctgtagc tcaggttgcc gaagtgaaga cgattcagtt tgcggtgggt
    16381 aagagcggta aaatatcggt ggttgcgtca ctcgcacctg tcatgcttga tgataaaaaa
    16441 gtccagcggg tgaatattgg ttccgtcagg cgctggcagg agtgggatat tgcgcctggt
    16501 gatcagatcc tcgtcagcct tgccggtcag ggtattcctc gcattgatga tgtggtgtgg
    16561 cgcggtgcag aacgtacaaa accgacaccg ccagaaaacc gctttaactc gttgacctgc
    16621 tactttgctt ctgatgtttg tcaggaacag ttcatttcac gcttagtctg gctggggtca
    16681 aaacaggttc ttgggctgga tggcattggt gaggccggct ggcgcgcgct gcatcagact
    16741 catcgctttg agcatatctt ttcctggctt ttattaacgc cagagcaatt acagaacacg
    16801 ccggggatcg cgaaaagtaa aagtgcgcag ctatggcatc agtttaatct ggctcgtaag
    16861 cagcctttta ctcgctgggt gatggcaatg ggaataccgc taacccgggc ggcgcttaat
    16921 gccagtgatg aacggtcctg gtcgcaactt ttattgagca cggagcagtt ctggcagcag
    16981 ctgccgggga ctggatcggg gcgcgccaga caggttattg aatggaagga aaatgcgcaa
    17041 atcaagaagc tgggcagttg gctggctgcc cagcagatca caggttttga accttagtga
    17101 ccttcgtggc tgtagtaaaa gaccggtaat cccagcttaa ggcgtagcgc cagtaagcgg
    17161 gcaaaaaagc cgaataccag ggtggagata attaccacat catggttaga aacatagtgt
    17221 tgcagcgcga tgtacagcac ggcggaggca aatgagacac cggcatacaa ctctttctgg
    17281 aataccagtg ggatgcgttt acagaacata tcgcgcaaaa cgccgccaaa tacgccggtc
    17341 gtcacagctg cgacaacggc aatgatcggg ccgtgtccca tatccagcgc aacctgtgcg
    17401 ccaatgatag aaaagaccac cagtccgagc gcatcgagca ccagaaacac tttgcgcagg
    17461 taaggcatta caggagcaac gatagtggtg agtactgcgg cggtagcgac aataatcaca
    17521 tattccgggt gtttgaccca gccgagcgga tagtggccca gcagaatatc gcgcactgac
    17581 cctccgccaa ttgccgtggc ggtcgcaata ataattacgc caaatgtatc catgcgccgt
    17641 cgtccggccg ccagcgcccc cgtcatggct tcggcagtaa tgccaaccaa atacaaaatg
    17701 tgtaacagca tggttatctc cctaaaaatc tgtctggcag ggtagcgatt tgtacgcatt
    17761 gtcacgattg agatttttta aggtgagagg aaattgataa tttttagtta tgtgaaatcg
    17821 ttgttttctg tagcgtagat gcattcttct gacttcttgt ctgagaaaat acataagaaa
    17881 aagtaggtta aaactgaggg gtggaaaaga aaggtgcaat gttagccatt gtgttcaccg
    17941 cacctggact ttggcttacg catcgggtat ctcaatcaac aaaaagagtt tttagtttct
    18001 gctatttctt ctctgtaatt tttacactgt tttccaactg cttgatgctg acctgcgagg
    18061 tcggcaactc ctcaggcatg gttccgccaa gttcctgaat ggtttgcctc accttgctac
    18121 ccacgtcaaa gtgtgtggtg ttagcttgct gttttgaatt cacgccatcc cgcttgagtt
    18181 tttcttctgt ttgggtagct cgaaagagat tagccgccag ttctgttgaa cccatatgat
    18241 ccaggatctt ctgactcttt ttcagcccct tccgctgatg gatagctttc tgatctaatc
    18301 caccatacag tccccggtaa ccatgattct gaaatatggc gaaatcgata gctgtcgcta
    18361 ccccagcttg ctgcgctgcc tcaactaatt gtttgttatg ttctttcaac tcatttctca
    18421 gaaaaagacg cttttcgtct tcacgaagtt gcctgaaagc ttcatcgtca gccagctcct
    18481 gccgtcgggt ctggatagca aaataagttt gccctgccgc aataaccggt ttcgcagggt
    18541 cgccgttttg tactaccagg taacaggcat agcgggagag tacgatgtct tttaatgctc
    18601 ttttggcatt tgagccaatt ttgaccatat cgaggatctc ctcgaaatgg tcatcaattg
    18661 tgtgaccact attcagacaa gcttctttgg cgcgcgttaa tacaggtata aagtgacgat
    18721 actctgaata acccagtagt tttccaagtt gtcttgctga ccattgttct gctccgtttg
    18781 cattaatcag ctttatctct tcaaaaggtt gatgatgttc gttcattgcc cactccatgt
    18841 gatatcgatt gcaaggaaag taagtctaca actgtgttta tatacagttg taagtatctc
    18901 acgttctcac tgcgctgggc aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag
    18961 aattgaatgt tgcggggagg aaaaatgccg ctctgtttga gtgggcggca tttgtgaagg
    19021 ctgaaaagga agaattggcg ttacgagtta ttcaatgttc tggatctgct cgcgcatctg
    19081 ctcaatcaac actttcagct cgatggcaga attcgtcact tcggcattga tagatttcga
    19141 cgcaagagtg ttcgactcgc ggttgaactc ctgcatcata aagtccagac gacgaccaac
    19201 cgcttctttt ttcttcagaa tgttgtaggt ctctttgaca tgcgcttcga ggcgatccag
    19261 ttcttcggca acgtcaattc gttgtgccag cagaaccaac tcctgctcca gacggttgtt
    19321 ttccagttgc acctgagcat cttccagctt cgcgaccaga cgctcacgct gccattgcag
    19381 gatttccggc atatgggcgc ggactttgac cacttcggcg gtgacgcctt ccagacgctg
    19441 ctcgatcaat gctttcagcg cctgaccttc ggtttcgcgt gcgacgataa agtcgtccag
    19501 cgtaccatcc agcgccgcga gaatttcagc ggtaatcgcg tcgagatcct gctcctgggc
    19561 tgccattacc cccggccagc ggagaatatc aaccgggttg atttcccctt cgtcactctg
    19621 catttttacc cagttcgcgg cagttaccag ctgtttagcc agtttttcgt tgaggatcag
    19681 ctcaccttgt gcgctaacat ctggttcata gcgcagggta cattccactt taccgcgcgt
    19741 caggcgagaa cgaatacgct cgcgaacgac aggttcaagg ctacggaact gctccggcag
    19801 acgaaagtaa gtttccagat aacgctggtt taccgagcgc atttcccagg ttgcgctccc
    19861 ccattcaccc ttgatttcac gccgggcgta ggcggtcata ctgcggatca tagacgttcc
    19921 tgtttataaa aggagaggtg gaaggattat agccatcgat gccttgtcag gataggaata
    19981 accgccggaa gtccgtataa tgcgcagcca catttgtttc aagccggaga tttcaatatg
    20041 cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta ccctgactcg taactataca
    20101 aaacatgcag aaggctcggt gctggtcgaa tttggcgata ccaaagtatt gtgtaccgcc
    20161 tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg gccagggctg gatcaccgca
    20221 gagtacggca tgctgccacg ttctacccac acccgtaacg cgcgtgaagc ggcgaaaggt
    20281 aagcagggtg gacgcaccat ggaaatccag cgtctgatcg cccgtgctct tcgcgcggca
    20341 gtagatttga aagcgctggg tgagttcact attacgctgg actgcgacgt gcttcaggct
    20401 gatggtggca cgcgtaccgc gtcgattacg ggtgcctgcg tggcgctggc agatgcgcta
    20461 cagaagctgg tggaaaacgg caagctgaaa accaacccga tgaaagggat ggtagccgcg
    20521 gtttctgtcg ggattgtgaa cggcgaagcg gtttgcgatc tggaatacgt tgaagactca
    20581 gccgcagaga ccgacatgaa cgtagtgatg accgaagacg ggcgtatcat tgaagtgcag
    20641 ggaacggcag aaggcgagcc gttcacccat gaagagctac tcaccttgtt ggcgctggcc
    20701 cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg cgctggcaaa ctgattttta
    20761 aggcgactga tgagtcgcct tttttttgtc tgtagaaaag taagatgagg agcgaaggca
    20821 tgaaaccata tcagcgccag tttattgaat ttgcgcttag caagcaggtg ttaaagtttg
    20881 gcgagtttac gctgaagtcc gggcgcaaaa gcccctattt cttcaacgcc gggctgttta
    20941 ataccgggcg cgatctggca ctgttaggcc gtttttacgc tgaagcgttg gtggattccg
    21001 gcattgagtt cgatctgctg tttggccctg cttacaaagg gatcccgatt gcgaccacta
    21061 ccgccgtggc gctggcggag catcatgacc ttgacctgcc gtactgcttt aaccgcaaag
    21121 aggcaaaaga ccacggtgaa ggcggcaatc tggttggtag cgcgttacaa ggacgcgtaa
    21181 tgctggtaga tgatgtgatc accgccggaa cggcgatccg cgagtcgatg gaaattattc
    21241 aggccaatgg cgcgacgctt gctggcgtat tgatttcgct cgatcgtcag gaacgggggc
    21301 gcggcgagat ttcggcaatt caggaagttg agcgtgatta caactgcaaa gtgatctcta
    21361 tcatcacctt gaaagacctg attgcttacc tggaagagaa gccggaaatg gcggaacatc
    21421 tggcggcggt taaggcctat cgcgaagagt tcggcgttta aagaaactcg ccggatgaaa
    21481 agtcatccgg cgtcatatta ctgcaactgt gcggcaatta gcggccagcg ggcgtcaaaa
    21541 tcatccgtcg ggcggtattt aaactcgctg cggacaaagc gtgacagcat accttcacag
    21601 aaggccagga tctggcttgc cagcagggtt tcatcggtgg tgtaaccttc accctcacgc
    21661 attctctttt cgcgcaatac ctgacgcaac tgcgcttcaa tacgctcgaa cagctggttg
    21721 atgcgccctt gcaggcgatc ctgttcaaac attagtgcat gaccagtgag gatgcgggtc
    21781 aggccaggat tacgctcacc aaaaccgaga agcagcaaca caatcagacg caggcgcgct
    21841 gtggtgtcct tctcatcttt cagaatcaga ttgatgcgag taatcaggct atcttcgata
    21901 aactcaatca ggctatcgaa catgcgggtc ttactgggga agtggcgata cagtgccgct
    21961 tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga tacgttggct tccatcgctg
    22021 gattccagca tcagcgccag agactgaagt atttcctcgc gacggttcct tttcgcagtt
    22081 tgtttttctg ccatgttaca aaatacccct gaaaataagc acttgccagg cggcacccac
    22141 gctatgaccg caaacgaaat gtttgcggct atgttatgac gttattcgga tgcgtatgtg
    22201 ttactgacga ccagagtgac caaagccgcc ttcaccgcgg tcggtggcgt cgaaatcttc
    22261 caccagatta aattcagcct gtactaccgg aacaaaaatc atctgggcga tgcgttcgcc
    22321 aggttgaatg gtgaagctgt cctgaccacg gttccacacg gaaatcatca actggccctg
    22381 atagtcagaa tcgatcaatc ctaccaggtt accaagcacg ataccgtgct tatgtcccaa
    22441 tccggagcgc ggcagcatca ttgccgccag tgaaggatcg gcaatatgaa tcgccagccc
    22501 ggtcggaacc agcgtagtgt cacccggagc cagttctacg gcgtcgtcga gacaggcacg
    22561 caggtcaagt ccggcagagc cagaggtggc ataagtcggg agcggaaatt ccttcccaac
    22621 gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata acgggtcacg atctcgtcga
    22681 gtaataattg gccaaggagc tctttgcgct caagcggtaa gactttatct ccgtcctgcc
    22741 agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg agttggctgg gaaacatcgt
    22801 tcgcgcagat cagatcaagg tttttacgga tacgtttttg ccgggcgtat tcttccacat
    22861 tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg gtcttttagt gcggcaacgc
    22921 ctgcgacgat atcggggttt ttaaccattt ttattgttaa ttcatcaccc tgcgtggcct
    22981 gctttttgat tttctctggg gccacggtag ctgcgcgata atccgccacg gcggcgcaac
    23041 cgataaaaat attttgctgc tgtacagaag cattcacggc cgcttccatt tccagcgcag
    23101 tcatcacatc aacacgttta acaaacggtg gcgtcggtag tgaaaccgga cctgatacca
    23161 gcgtgacgtt cgcgccacga cgggcagcgg cggcggcgat agcaaaaccc atcttgcctg
    23221 agctgtgatt agagatataa cgcaccggat cgagcggttc acgcgtcggg ccggcggtaa
    23281 tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa atacgctacc gccatatcaa
    23341 caatggttaa cggatcgagc attcgcccag ggccgatatc accacaagcc tgactgccac
    23401 tgtctggccc ccagatgagc aaaccacggg aagcaagcac ctctaaatta tgctgcgtgg
    23461 cagcggcacg gtacatctgc tggttcatgg cggggagcac ggctacaggc gcaggtgtgg
    23521 ccagacaaat cgtcgatacc agatcattcg ccattccggc agcaacacgg gcaatcaaat
    23581 ctgccgtggc aggggcgaga atcactaaat cagcccattt acccagctca atatggccca
    23641 tagcggcttc ggctgccggg tccagcagac tgtcggaaac gggataacca gaaaccgcct
    23701 gcaagctaag tggggtgata aaggcttttg ccgcttcggt catggctacg cggacgtcgg
    23761 ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt ataggcagca ataccgccgc
    23821 tgacgccgag aacgattttt ttaccggcca ggctcatgat gatttttcct gttgggtgac
    23881 accagaagtt ggcgatttta tcacaatact tttgttgtcg tgccttcacc tgagaatcac
    23941 tttgcgaggc gctttccagg attgaaaact ggccgtcgat ttaacggaac ggctatgaca
    24001 ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat gcacagctgt tgatgccgcg
    24061 cgaaaaaatg ctgaagtttg gtattagcgc cttaacggat gtcgagctgc tggcgctatt
    24121 tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg gcaaaagaga tgctggagaa
    24181 tttcggctct ctttatggct tgttaacctc tgaatatgaa caatttagtg gcgttcatgg
    24241 aattggcgtg gcgaaatttg cccagttaaa ggggattgct gaactggcgc ggcgttacta
    24301 caacgtgcgg atgcgtgaag aaagcccttt actcagcccg gagatgacga gggaattttt
    24361 acaaagccag ctcacgggtg aagagcggga gatctttatg gtgatctttc tcgactcaca
    24421 acaccgggtt ataacgcata gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca
    24481 tcctcgggaa attatccgcg aagcgataaa aataaatgcc tcggcgctga tccttgcaca
    24541 taatcaccct tcgggttgtg ctgaacccag taaagcggat aaactcatta ctgaacggat
    24601 aataaagagt tgtcagttca tggatttacg cgtgctcgac catatcgtga ttgggcgtgg
    24661 agagtatgtt tcttttgccg aacgcggctg gatttaaccc gctatgcgcg atccttcggg
    24721 atctttgtct gttcgggact tgagcacatc gctgagtcag cgtatactac gccacctttg
    24781 agaatctcgg gtttggcatt tgggcctggc aatcgagagt tcacatagaa ctgcgatgac
    24841 cgggctgtaa agcctgacga ggcgccaata ccccatacga agctcgagct aatttgattt
    24901 ttggagaata gacatgtccc gagtctgcca agttactggc aagcgtccgg tgaccggtaa
    24961 caaccgttcc cacgcactga acgcgactaa acgccgtttc ctgccgaacc tgcactctca
    25021 ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat
    25081 gcgtgtaatc gataaaaaag gcatcgatac agttctggct gaactgcgtg cccgtggcga
    25141 aaagtactaa gtacttagag gaaataaatc atggctaaag gtattcgtga gaaaatcaag
    25201 ctggtttctt ctgctggtac tggtcacttc tataccacta cgaagaacaa acgtactaag
    25261 ccggaaaaac tggaactgaa aaaattcgat ccagttgttc gccagcacgt gatctacaaa
    25321 gaagcgaaaa tcaaataatt tttgctttga tgtaacgaaa aacctcgccc cggcggggtt
    25381 ttttgttatc tgcctgcccc catattgact gcatctgttc attcctggag atgctatgcc
    25441 tgaattaccc gaagttgaaa ccagccgtcg cggcatagaa ccgcatctcg ttggtgcaac
    25501 cattcttcat gcggtggtgc gcaacggacg cttgcgctgg ccggtttcag aagagatcta
    25561 ccgtttaagc gaccaaccag tgcttagcgt gcagcggcgg gctaaatatc tgctgctgga
    25621 gctgcctgag ggctggatta tcattcattt ggggatgtct ggcagtctgc gcatccttcc
    25681 agaagaactt ccccctgaaa agcatgacca tgtggatttg gtgatgagca acggtaaagt
    25741 gctgcgctac accgatccgc gccgctttgg tgcctggcta tggaccaaag agctggaagg
    25801 gcacaacgtt ctggcccatc ttggaccgga gccgcttagt gacgatttca acggtgagta
    25861 cctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa ccgtggctga tggataacaa
    25921 gctggtggta ggcgtaggga atatctatgc cagcgaatct ctgtttgcgg cggggatcca
    25981 tccggatcgg ctggcgtcat cactgtcgct ggcagagtgt gaattgttag ctcgggtgat
    26041 taaagcggtg ttgctgcgtt cgattgagca gggtggtaca acgctgaaag attttctgca
    26101 aagtgatggt aaaccgggct atttcgcgca ggaattgcag gtttacgggc gaaaaggcga
    26161 accctgtcgg gtgtgcggta cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt
    26221 ttattgtcgg cagtgccaga agtaattcat gcgcgccgga tggcatacca tccggcataa
    26281 acgctacgct aacttcgcca tcagcgcctg atggacattc tccggcagga aatgggtgac
    26341 atcgccctga tggcgcgcca cctctttcac caacgatgaa gagataaacg accactcttt
    26401 cgacggcatc agaaacacac tttccagttc cggcattaag tggcgattca tatgcgccag
    26461 ctgcatttca tattcaaaat ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg
    26521 ttgattgcgg gcgaagttcg ccatcaaatc actaaacccg accacttcca cgttccccag
    26581 atgcgcggtt gcctgctgtg ccagctccac acgctcttcc agggtaaaca tcggtttttt
    26641 actggggctg gcggcaatcg ccagaataac gtgatcgaac atttgcgtgg cgcgcgtcac
    26701 gatatcgata tgaccattgg taatgggatc gaaagtaccc ggataaatcg cccgtttttg
    26761 cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc cagcagttga agcagacgct
    26821 gtagcgcgcc ctggttttga tacagtactt caacggcatg acggccatag aaactacggt
    26881 aatcggcgtc ggtgagtaaa gaggaaacct cttttgcaag cgtagtggca tcggtaacgg
    26941 taatcagccc gcttgcctgc tccagccgcg cgcaaatgtc tttaaagtta aaagtatgcg
    27001 gccccatcaa taccggaata gcgtgtgcgg cagcttccag cggattatgc ccaccacgtt
    27061 caaccagtga accgccaaca aaggcgagat cggcaatgcc atacagtaac atcagctcgc
    27121 ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg gacttcccct gaagagcgtg
    27181 tgatatagct tagtccagcc tggcggacaa ggttaatcgc atccgggaag cgttccggat
    27241 gacggggtac caggatgagc aataaattcg ggaattgctg taacaatgcc tgatgtgcgg
    27301 cgatcaccac actctcttcg ccttcgtgag tgctggtggc aatccatacc gggcggtgtg
    27361 gtgcccactg gcggcgcagc gtcactgctt tagcagccag ttgcggcgtt acagaaatat
    27421 cgaatttcag gctaccggta acggtcacct gattattttt tgcgcccagt gatacaaaac
    27481 gtgcaccatc ttcttcattt tgcgcagcaa tcagcgtaat acgacgcagc aagcgacgga
    27541 cgaatttgcc cagtttggca taacctgcgg ccgagcgggc agagagtcgc gcgttagcga
    27601 tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag gttaggccat agttcggttt
    27661 ccataatcaa cactagttta gggtcgactt tattcaggaa acgattaagt gcatcgggca
    27721 ggtcatacgg cagataaacg tgctgaacat ccttcccgaa agccgattgt acgcgctccg
    27781 aaccggttgg cgtcatggtt gttacggtaa tcggtaaatc aggataacga tgacgcagtg
    27841 cgcgcaccaa cgggatcgcc gccagagttt caccgacgga gacggagtgc agcataatgc
    27901 cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc accccagcgt tttcgatagg
    27961 ccggagcctt acgtccgcgc acccagagcc gtatccagat cagcggctga ataaggtaga
    28021 gaagggcggt gtaaagcaat tcgagcatag taaatagctg acttatggat gtgctgggga
    28081 ttctatgtat ttagctgtgg ctttaccatt acttttcccg tttttgactt aaatagcaat
    28141 ttacccgcca attcttgctg aacatctata ctttttttgt atttttatgc gattcattga
    28201 tttctgcagt acgtgcagga aggaatcgca gagcaacagt gaaagaatat ggttgctaat
    28261 gattttattt gattgatttc tttattgaaa atcaacaatg tgcttaaaaa tcattcgggt
    28321 cggattgccg cgaaagaagt gatacactag cgcgtcaata tcagtgcggt atcagtattc
    28381 aggtagctgt tgagcctggg gcggtagcgt gctttttctg cttcacctta atcggataat
    28441 ctcaacaaaa agagttactt gtggataagc catttcgaag aattttgctc attaagatgc
    28501 gttttcatgg ggatatgtta ttaactactc ccgtcattag ttcgctgaaa aaaaattacc
    28561 ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat cccgatcctg tctgaaaatc
    28621 cagagattaa cgcgctctac ggcataaaaa ataaaaaagc aaaagccgca gaaaaaattg
    28681 ccaacttttt tcatctcatc aaggtattac gtgccaataa gtatgacctt atcgttaatc
    28741 ttaccgatca atggatggtt gctatactgg tgcgcttatt aaatgcccgt gtgaaaattt
    28801 cccaggatta tcatcatcgg caggctgctt tttggcgtaa tagtttcact catttggtgc
    28861 cgttgcaggg tggaaatgtg gtggaaagta acttatccgt gctgtcgcca ttggggcttg
    28921 aatcgttggt gaagcagaca accatgagtt acccgcctgc aagctggaaa cgtatgcgtc
    28981 gcgaacttga tcacgctggt gttggacaaa attatgtggt tatccaacct acggcgcggc
    29041 aaatcttcaa atgctgggac aacgccaagt tttccgctgt gattgatgcc ttacatgctc
    29101 gtggttatga agtcgttctg acgtccggcc cagataaaga cgatctggtc tgcgtcaatg
    29161 aaattgcgca gggatgccag acgccaccag taacggcgct ggccggaaag gtgaccttcc
    29221 cggaacttgg tgcgttaatc gatcatgcgc agctgtttat tggcgttgat tccgcaccgg
    29281 cgcatattgc cgcagcagtt aatacgccgc tgatatcgct gtttggtgcg acagaccata
    29341 ttttctggcg tccctggtca aataacatga ttcaattctg ggcgggagat taccgggaaa
    29401 tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct ttcggttatt ccggcggcag
    29461 atgtcattgc tgctgtcgat aaattactgc cctcctccac gacaggtacg tcgttatgat
    29521 cgttgctttt tgtttatata aatattttcc ctttggcggt ttgcagcgcg attttatgcg
    29581 tattgctcag acagtcgccg cccgaggtca tcatgttcgg gtttataccc agtcgtggga
    29641 aggcgaatgt cctgatgtat ttgaactgat caaagtgccg gttaaatcgc ataccaatca
    29701 cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga
    29761 taaagtcgtt ggattcaaca aaatgccggg gctggacgtt tattatgccg ctgatgtttg
    29821 ttatgccgag aaagtagcgc aggaaaaagg ctttttctat cgcctgacgt cacgttatcg
    29881 ccattatgcc gcctttgagc gggcaacctt cgaacagggc aagccgacac agctgctgat
    29941 gctgacagat aagcaaatcg ccgatttcca gaaacattat cagactgaag tggagcgttt
    30001 tcatattctg ccaccgggga tttatcctga tcgtaaatat agccagcagc cagccaatag
    30061 ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa caatatttat tgttgcaggt
    30121 tggttcagac ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg cttcgttacc
    30181 ggattcgctg cgccacaaca cattgctata tgttgttggg caggataaac cgcgaaaatt
    30241 tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa
    30301 cgatgtctcg gaattaatgg cggcggcgga tttattactg catcctgcct accaggaagc
    30361 cgcgggaatt gtactgctgg aagcgataac tgcaggatta ccggtactaa caacagccgt
    30421 ttgtggctat gcgcattata ttgtcgacgc caattgcggc gaggctattg ctgagccatt
    30481 ccgccaggaa acattgaatg agattttacg caaagcgtta acgcaatctt cattgcgcca
    30541 ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa gatttataca gtctgccaga
    30601 gaaagcggcg gacatcataa cgggtggtct ggatggttga acttaaagag ccgtttgcca
    30661 cgttatggcg cggtaaagat ccttttgagg aagttaaaac cttgcagggt gaggtatttc
    30721 gtgaactgga aactcgccgc actctgcgct ttgaaatggc gggcaaaagc tattttctca
    30781 aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa tttactctca ttgcggatgc
    30841 cagtattagg cgcagaccgc gaatggaatg cgattcatcg actgcgggat gtcggcgttg
    30901 atactatgta tggggtggca ttcggcgaaa aaggcattaa tccgctcacc agaacctcgt
    30961 ttattataac tgaagatctg acaccaacca tcagtctgga agattactgt gctgactggg
    31021 cgactaaccc accagatgtt cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg
    31081 tgcgcgatat gcatgctgcg ggcattaacc accgtgactg ttatatctgt catttcctgc
    31141 tgcacttgcc tttttccggt aaggaagagg agttaaaaat ttcggtaatt gacctgcacc
    31201 gggcgcagct tcgcacgcgc gttccacgtc gttggcgcga taaagatctt atagggcttt
    31261 atttttcttc gatgaatatt ggcctgactc agcgggatat ctggcggttt atgaaagtgt
    31321 attttgccgc cccgcttaaa gacattctca agcaggaaca aggactgctg tcgcaagcag
    31381 aagcaaaagc cacaaaaatc agggaaagaa cgattagaaa atcgttgtaa cccgtaactt
    31441 atttttgcca aaattttgga tacagaataa atatgtctca actcaatgat agtgacatca
    31501 tcctttttga gtataacttt cattatcaaa atataagatc taaaaatact cttgatatcg
    31561 catttggtat tgacagaaat tttctttttg gatgtggtgt agccatcgca tctattctat
    31621 taaacaatag cgaaatctct t
//
LOCUS       DAYPHN010000040        30828 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000040 DAYPHN010000000
VERSION     DAYPHN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.040"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(46..422)
                     /gene="rnpB"
                     /locus_tag="ACG0BW_001785"
     ncRNA           complement(46..422)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG0BW_001785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(455..1600)
                     /gene="garK"
                     /locus_tag="ACG0BW_001786"
     CDS             complement(455..1600)
                     /gene="garK"
                     /locus_tag="ACG0BW_001786"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFK7258757.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNVG"
     gene            complement(1697..2587)
                     /gene="garR"
                     /locus_tag="ACG0BW_001787"
     CDS             complement(1697..2587)
                     /gene="garR"
                     /locus_tag="ACG0BW_001787"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFK7258758.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQAVRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2617..3387)
                     /gene="garL"
                     /locus_tag="ACG0BW_001788"
     CDS             complement(2617..3387)
                     /gene="garL"
                     /locus_tag="ACG0BW_001788"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFK7258759.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3403..4737)
                     /gene="garP"
                     /locus_tag="ACG0BW_001789"
     CDS             complement(3403..4737)
                     /gene="garP"
                     /locus_tag="ACG0BW_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFK7258760.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5112..6683
                     /gene="garD"
                     /locus_tag="ACG0BW_001790"
     CDS             5112..6683
                     /gene="garD"
                     /locus_tag="ACG0BW_001790"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFK7258761.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6832..7167
                     /gene="prlF"
                     /locus_tag="ACG0BW_001791"
     CDS             6832..7167
                     /gene="prlF"
                     /locus_tag="ACG0BW_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFK7258762.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNTPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7167..7631
                     /gene="yhaV"
                     /locus_tag="ACG0BW_001792"
     CDS             7167..7631
                     /gene="yhaV"
                     /locus_tag="ACG0BW_001792"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFK7258763.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH"
     gene            complement(7686..8495)
                     /gene="agaR"
                     /locus_tag="ACG0BW_001793"
     CDS             complement(7686..8495)
                     /gene="agaR"
                     /locus_tag="ACG0BW_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFK7258764.1"
                     /translation="MSNTDASGEKRMTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8744..10024
                     /gene="kbaZ"
                     /locus_tag="ACG0BW_001794"
     CDS             8744..10024
                     /gene="kbaZ"
                     /locus_tag="ACG0BW_001794"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFK7258765.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPVPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10047..10520
                     /gene="agaV"
                     /locus_tag="ACG0BW_001795"
     CDS             10047..10520
                     /gene="agaV"
                     /locus_tag="ACG0BW_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFK7258766.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10531..11310
                     /gene="agaW"
                     /locus_tag="ACG0BW_001796"
     CDS             10531..11310
                     /gene="agaW"
                     /locus_tag="ACG0BW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFK7258767.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11300..12178
                     /gene="agaE"
                     /locus_tag="ACG0BW_001797"
     CDS             11300..12178
                     /gene="agaE"
                     /locus_tag="ACG0BW_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFK7258768.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12196..12630
                     /gene="agaF"
                     /locus_tag="ACG0BW_001798"
     CDS             12196..12630
                     /gene="agaF"
                     /locus_tag="ACG0BW_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFK7258769.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAFAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12627..13760
                     /gene="nagA"
                     /locus_tag="ACG0BW_001799"
     CDS             12627..13760
                     /gene="nagA"
                     /locus_tag="ACG0BW_001799"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFK7258770.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELAGVTPAEAIHMASLH
                     PAQMLGVDDVLGSLKPGKRASIVALDSGLHVQQIWIQGQLASF"
     gene            14110..15264
                     /locus_tag="ACG0BW_001800"
     CDS             14110..15264
                     /locus_tag="ACG0BW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFK7258771.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRVILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMVSCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15277..16137
                     /gene="kbaY"
                     /locus_tag="ACG0BW_001801"
     CDS             15277..16137
                     /gene="kbaY"
                     /locus_tag="ACG0BW_001801"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFK7258772.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFDENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16304..16780
                     /gene="agaB"
                     /locus_tag="ACG0BW_001802"
     CDS             16304..16780
                     /gene="agaB"
                     /locus_tag="ACG0BW_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFK7258773.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16819..17622
                     /gene="agaC"
                     /locus_tag="ACG0BW_001803"
     CDS             16819..17622
                     /gene="agaC"
                     /locus_tag="ACG0BW_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFK7258774.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17612..18403
                     /gene="agaD"
                     /locus_tag="ACG0BW_001804"
     CDS             17612..18403
                     /gene="agaD"
                     /locus_tag="ACG0BW_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFK7258775.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18404..19171
                     /locus_tag="ACG0BW_001805"
     CDS             18404..19171
                     /locus_tag="ACG0BW_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFK7258776.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(19221..20084)
                     /gene="rsmI"
                     /locus_tag="ACG0BW_001806"
     CDS             complement(19221..20084)
                     /gene="rsmI"
                     /locus_tag="ACG0BW_001806"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFK7258777.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            20149..22185
                     /gene="lpoA"
                     /locus_tag="ACG0BW_001807"
     CDS             20149..22185
                     /gene="lpoA"
                     /locus_tag="ACG0BW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFK7258778.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALMLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPAATTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSHSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            22143..22538
                     /locus_tag="ACG0BW_001808"
     CDS             22143..22538
                     /locus_tag="ACG0BW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFK7258779.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            22558..23148
                     /gene="diaA"
                     /locus_tag="ACG0BW_001809"
     CDS             22558..23148
                     /gene="diaA"
                     /locus_tag="ACG0BW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFK7258780.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            23158..23733
                     /gene="dolP"
                     /locus_tag="ACG0BW_001810"
     CDS             23158..23733
                     /gene="dolP"
                     /locus_tag="ACG0BW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFK7258781.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(23855..24895)
                     /locus_tag="ACG0BW_001811"
     CDS             complement(23855..24895)
                     /locus_tag="ACG0BW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFK7258782.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWQFAAIRLVAGLAMVLLIATLVQKWVREIPLTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLA"
     gene            complement(24968..25603)
                     /locus_tag="ACG0BW_001812"
     CDS             complement(24968..25603)
                     /locus_tag="ACG0BW_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFK7258783.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGAKAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            25731..26249
                     /gene="yhbO"
                     /locus_tag="ACG0BW_001813"
     CDS             25731..26249
                     /gene="yhbO"
                     /locus_tag="ACG0BW_001813"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFK7258784.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(26229..26672)
                     /locus_tag="ACG0BW_001814"
     CDS             complement(26229..26672)
                     /locus_tag="ACG0BW_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFK7258785.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            26723..27025
                     /gene="yhbQ"
                     /locus_tag="ACG0BW_001815"
     CDS             26723..27025
                     /gene="yhbQ"
                     /locus_tag="ACG0BW_001815"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFK7258786.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKVKSD"
     gene            complement(27012..27515)
                     /locus_tag="ACG0BW_001816"
     CDS             complement(27012..27515)
                     /locus_tag="ACG0BW_001816"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7258787.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(27509..28033)
                     /locus_tag="ACG0BW_001817"
     CDS             complement(27509..28033)
                     /locus_tag="ACG0BW_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFK7258788.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            28242..29237
                     /locus_tag="ACG0BW_001818"
     CDS             28242..29237
                     /locus_tag="ACG0BW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFK7258789.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAIHFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            29246..30124
                     /locus_tag="ACG0BW_001819"
     CDS             29246..30124
                     /locus_tag="ACG0BW_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFK7258790.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASNADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFTMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            30205..>30828
                     /locus_tag="ACG0BW_001820"
     CDS             30205..>30828
                     /locus_tag="ACG0BW_001820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HFK7258791.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSN"
BASE COUNT         7421 a         8247 c         7951 g         7209 t
ORIGIN      
        1 gctccaaaaa gcaaaaaccc gccgaagcgg gtttttacgt aaatcaggtg aaactgaccg
       61 ataagccggg ttctgtcgtg gacagtcatt catctaggcc agcaatcgct cactggctca
      121 agcagcctac ccgggttcag tacgggccgt accttatgaa cccctatttg gccttgctcc
      181 gggtggagtt taccgtgcca cggactgtta ccagccgcgc ggtgcgctct taccgcaccc
      241 tttcaccctt acctgatccc gcttgcgcgg gccatcggcg gtttgctctc tgttgcactg
      301 gtcgtgggtt tcccccccag gcgttacctg gcaccctgcc ctatggagcc cggactttcc
      361 tcccctccgc ccgtctcccc cgaagaggac gacgacgaag cggcgactgt ctggtcagct
      421 tcggcgcgca gtatagaggg tttgcgcgcc cttgtcaccc cacgttgcgc attccaatcg
      481 ccagtgtcgc ggcgatatta cgtgaagcac ggtaaatatt gtcataagcc ccgcggaatg
      541 cttcgtccaa cgtaccaata ctggtcaata cgctgaagac cgcatcaatg ccatgctgat
      601 gtacaacgcc aacatcattg gtcaggctac ccgcaatgcc aatcaccggt ttatggtact
      661 ttttcgccac gtttgcgacg ccaatcggaa ccttgccgtg aatactctgg ctgtcaatac
      721 gcccttcacc ggtgatcacc agtgtacaat cgtgaatatg ttcctccaga ttcagcgccg
      781 tagtgacgat ttcaatacca cttttcagtt ccgcaccaag aaacgccatt agcgccgcgc
      841 ccataccacc cgcagctcct gcgccgggga catctttcac atcaacatgc agcgcttttt
      901 taatgacatc ggcatagtga gagaggttat tgtccagctc aacaatcatc gcttcactgg
      961 ctcccttttg tgggccaaag atgcgcgatg caccgttatc gcccaccagc ggattggtga
     1021 catcacaagc gacgcgaatg acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa
     1081 tatcattcag agtattaaga ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc
     1141 ataatttcgc ccccagcgcc tgtaccatgc ctgcaccgcc atcatttgta gcgctaccgc
     1201 caatgccgat aataatgttt gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg
     1261 tgccacgtga agtggtcacg agtggatcgc gtttttccgc aggtaccagc tccagcccac
     1321 tggccgccgc catttcaata aacgcggttt tgccatcgcc ggagatcccc caactggcat
     1381 tcaccttctc gcccagcggc cctgtaaccc aggcgtgacg ttcggaaccc tgggtggctg
     1441 caatcatcgc ttccaccgtt ccttcgccac cgtcggcaac cggaacagaa acgtactgtg
     1501 catcaggaaa aatttcccga aatccttttt ctatcgcctg cgcaacctcg ctggcagata
     1561 aactttcttt ataagagtct ggggcgatta cgattttcat acctatgcct gttaccacat
     1621 gacgccggag ggcgtttctc ttattcggcc tggattccag gcccggattg caatacgcca
     1681 tccgggcacg acgtcattaa cgagtaactt cgactttcgc cagtttttcg tagtagcacg
     1741 ccagggcgct atgatctgcc gttcctaaac catctgctcg cactgcctgc atcatctcca
     1801 taaccgcagc tgtgagcggc agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg
     1861 ccagatcctt aatatgcaga tcaatacgga agcccggctt gaagttgcgg tccatcacca
     1921 tcggcgcttt ggcatccagc acggtactgc ccgccagtcc accgcgaatt gcctgataca
     1981 ccaaatccgg gttaacgccc gctttggttg ccagcgttaa cgcttctgac atcgcggcaa
     2041 tattcagcgc cacaatgacc tgatttgcca gtttggtgac gttacctgca ccgatttccc
     2101 cggtatgcac cacggaaccc gccatcgctt tcatcaaatc atagtatttg tcgaaaatag
     2161 ccttgtcgcc gcccaccatc actgacagcg taccgtcgat agctttcggt tcaccgccgc
     2221 tcaccggagc atccagcata tcaatgcctt tcgctttcag cgcttcgctg atttcacggc
     2281 ttgccagcgg tgcgatagaa ctcatatcga tcaataccgt acctggcttc gcgccttcaa
     2341 taatgccatt ctcacccagc gccacctctt tcacatgagg ggagtttggt agcatggtta
     2401 tgatgacgtc gcactgttca gcgatcgctt tagccgtaga ggctgtttct gcacctgcag
     2461 caatcacgtc agcaatagct tctgggttac ggtcagcaac caccagcgag taacctgctt
     2521 tcagaaggtt tttactcatt ggtttaccca taatccccag gccaataaaa ccaactttca
     2581 tagtcatatc aatcatctct cttgttgcgg tggtggttat tttttaaagg tatcagccag
     2641 tttctgagtg gcagagcgga agacgccgag atcgctgccg acagccacaa acgtcgcacc
     2701 ccattccaga taacgacgcg catcggcttc gaccggtgcg aggataccgc tgggtttgcc
     2761 gtgcgcactg gcacggttaa aaatatgctg aattgctttt tgtacatccg ggtgcgaggc
     2821 attgccgaga tggcctaatg ccgcagccag atcgctgggg ccgacgaaga taccgtctac
     2881 gccttcggta gcggcaatgg catcgacgtt atctacgccc tgctgacttt ctatctggac
     2941 cagaatggtg atgttcttgt tcgactgagc gaaataatcc gccacggtgc caaacatatt
     3001 ggcgcggtga gaaacggaga cgccgcgaat gccttccggt gggtaacggg ttgatgccac
     3061 cgcctgctct gcttcctctt ttgtttctac aaaaggaatc aggaagttat agaaaccgat
     3121 atccagaaga cgcttaataa ttaccggctc gttggtcggc actcgcacta ctggcgcgct
     3181 ggcgctgcct ttcagggcca ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg
     3241 ttcgccatcc agcaccagcc agtcaaaccc agccaaacca agaacttcag tgctaatcgg
     3301 gttagagagt gctgaccagc aaccaatttg tacctgtttc gcagccagtg cggctttgaa
     3361 tttattcggg aaaacatcgt tattcatcgc ttataccttt gcttatttct gcaattccat
     3421 acgtttaatg tcgccaacta caaagaggta gcagaccatc gccatcagcg ctgaacatcc
     3481 cacgaaaacc agtgctgcat tgaaggagtg cagttcactt accagatagc caatcaccag
     3541 tggagtgaca atggaggcaa cattgccaaa gacgttaaag acgccgccgc agaggccaac
     3601 aatctctttc ggcgcggtgt cagaaattac cggccagccc agcgcaccaa atcctttgcc
     3661 aaagaaagcc agcgccatca gcatgaccac cagcgtggtg ttgttggtgt agttacataa
     3721 gatgatggtg gaagccagca acattcccag cacaatcggt agcttacgtg ccagggtcag
     3781 ggacaaaccg cgtttgatca gataatccga gaagacacct cccagcacgc cgcccgcaaa
     3841 gccacacagt gccggaatcg aggcgaccag acccactttc agaatcgaca tgcctttttc
     3901 ctgcaccaga taaattggga accaggtgag gaaaaaccag gtgatggtgt tgataaaata
     3961 ttgtccgaaa aatacgccca gcatcatgcg gttagagagc aattgcttga tgtaatgcag
     4021 tttgggaccg cttgctgccg cactgcccgg ctttttatgg tccatatcga ccaccgcgcc
     4081 attttctgag ataaacttca gctcttccgc agacatacgt gggtgatctg tcgggttatg
     4141 aatcaacttg atccacagcg ccgtcagcac aaaaccaatt acccccataa cggtaaagac
     4201 gtgctcccag ccccaggcga aagtcagcca gccaagcagc ggcgaaaaga gcgccagcga
     4261 gaaatattgc gccgagttaa agatggcgga ggcagtacca cgttctttcg tcgggaacca
     4321 ggcggcgaca atacgggcgt tcgccgggaa tgatggcgct tccgagaacc ccagcataaa
     4381 gcgcataaag aacatggaga tccctgccca ggccaacggg aacatatcaa caaagccttg
     4441 caggaaggtg aacagcgacc agaaaaagag gctgtaggtg taaacttttt tcgagccaaa
     4501 cttatcaagc aaccagccgc cggggatttg catcagcagg taggcccagc caaaagcgga
     4561 gaagatgtaa cccatcgaaa ccgcacttaa ctgcaactct tttgccactt cggtaccagc
     4621 aatagacagc gttgcacgat cggcgtagtt aacggcggta acaataaaaa taatcagtaa
     4681 tattaaatag cgggtatgca cgcctttctt ttttacgtca acggtgtcca gaatcatttt
     4741 atttacctcg ggtacttatg ctgattttta ttattatggg gaaggtgtta tttatgagtt
     4801 ttatttatgc cgttacggca atgaactcgg gaattagtat aagcagcgcg agaataataa
     4861 tcattgtgca aatgctaatt taattaatac tatttaaata ttattttgag catatgcaca
     4921 taaggttgcg cgctaaagca cagatttgcg ctttacctta ccgtgcggca ctgcaatccc
     4981 tgaaatgatt gacattgatc acatttctgc gtttaaactc ctgacattct tatttcaccc
     5041 aatgaagtca tttattttta aatgagacca ggtcctcatt ttaataaccc ctggctggag
     5101 aatattgcac aatggccaac atcgaaatca gacaagaaac gccaactgcg ttttatataa
     5161 aagttcacga cacagataat gtggcaatta ttgttaatga taatggcctg aaagcaggaa
     5221 cgcgttttcc ggatgggctg gaattaattg aacatattcc ccaggggcat aaagtcgcat
     5281 tgctggacat tccggctaat ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg
     5341 tgcgcgcaat cccacgcgga agctggatcg acgaatcaat ggttgtacta ccggaagcgc
     5401 cgccgttaca cacgctgcca ctggcaacca aagtcccggc ccccttaccg ccactggaag
     5461 gatacacctt tgagggctat cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg
     5521 gcatcaccac cagcgtccac tgtgtggcag gcgtggtgga ctatgtagta aaaatcattg
     5581 aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt ggtggggctg aatcatttgt
     5641 acggttgtgg cgtggcgatt aacgcaccgg cggcagttgt gcctattcgt accattcaca
     5701 atatttcgct gaaccctaac tttggcggcg aagtaatggt gattggtctg ggttgtgaaa
     5761 agttgcagcc tgaacgcctg ctgactggaa cggatgatgt gcaagctatt ccagtagaaa
     5821 gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg
     5881 atattttgca ggtcgccgaa cgccatttgc acaaactgaa tcaacggcag cgagaaacct
     5941 gtccggcttc agaactggtt gtcggcatgc agtgcggcgg tagcgatgcg ttttctggcg
     6001 taacggcaaa cccggcggtt gggtatgcgt ctgatctact ggtgcgctgc ggcgcaacgg
     6061 tgatgttttc agaagtcacg gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag
     6121 tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga gtggtacgat aactatctca
     6181 atatgggaaa aaccgaccgc agcgccaacc cttcgccggg caacaagaaa ggcggtctgg
     6241 caaacgtggt ggagaaagcg ctcggctcca ttgctaaatc gggtaaaagt gcaattgttg
     6301 aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt aatttacgcc gcgacgccag
     6361 ccagtgattt tgtctgtggc acacaacagg tggcttcagg gattaccgtg caagtgttta
     6421 cgaccggtcg cggtacgccg tacggcctga tggcggtacc cgtcattaaa atggcgaccc
     6481 gcaccgagct ggcgaaccgc tggtttgatt taatggatat taatgcgggc accatcgcta
     6541 ccggcgaaga aactattgaa gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg
     6601 ccagcgggaa gaagaaaacc ttctcagatc aatggggact gcataaccag ctggcggtgt
     6661 ttaacccggc accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac atggccttag
     6721 tgccatgttt ttattgttta aagtccccac gtccattaat aatgcatttg cattacctta
     6781 gttcaagctt ataatttgag cagaaaacag gctgtaaaag gacagtgaat catgcccgct
     6841 aatgctcgct ctcacgctgt actgaccact gaatcaaagg tcacgatacg cggacaaaca
     6901 actatccccg cgccagtgcg tgaggcctta aaactgaagc caggccagga cagcattcat
     6961 tacgaaattc tgcctggtgg gcaagtattt atgtgccgac tgggagatga acaggaggat
     7021 catactatga atgcattttt gcgttttctg gatgcagata tccagaacac cccgcaaaaa
     7081 actcgtccat tcaacattca acaaggaaag aaacttgtcg ctggcatgga cgtcaacatt
     7141 gatgatgaga ttggcgacga cgaataatgg attttccaca aagggttaat ggttgggcgc
     7201 tatatgctca tccctgtttt caggaaacct acgacgcttt agttgccgaa gtcgagacat
     7261 taaagggaaa agatcctgaa aattatcaga gaaaagccgc cacaaagtta ttggcggtag
     7321 tccataaagt gattgaggag catatcacgg tcaatccatc atcaccggca ttccgtcatg
     7381 gcaagtcgtt aggctctggg aaaaataaag actggtcacg ggtaaaattt ggtgctggtc
     7441 gttatcgtct cttctttcgt tatagtgaaa aagagaaagt catcattctg ggatggatga
     7501 acgatgaaaa cactctgcgc acctacggta aaaaaacaga tgcttatacc gtattcagca
     7561 aaatgttaaa aagaggacat cctcctgccg actgggaaac cctcacccga gaggcagaag
     7621 aaacccattg atggtgttca caatgcccgc cgaacgcatt cgtgttgacc agcgggcacc
     7681 ctttctcact ccccgaccag aatcacttca accccagcct ttcgcaggcc ttccagacta
     7741 tccgcaggaa tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta
     7801 tgtaaactgg aacgattgaa cttactggaa tcggtgacca cgatgatccg ttccgcaact
     7861 tcgcacatcc ggcggtttaa acgggcttca tcttcattat gcgtactgac gccgcgctcc
     7921 agatcgatcg catctacgcc aagaaacagc atatcgaagt ggtaattttg cagcgattgt
     7981 tcagcctgat cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc ggtcatcagc
     8041 agctcaacgc cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca
     8101 attacgtcag tgtgcttgcg catcagacga gcaatctcaa aagtggtggt cccggaatcg
     8161 aggatcaccc gatgacctgg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc
     8221 atcgcggtgt tcagcgcgct tttatcttcc actgatggct cgactgacgg cgtggtgcta
     8281 tcgcagatca acgcgccgcc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc
     8341 agatcgttgc ggatcgtcac agtagatacg ccatacaatg ccgacagatc gttaacctgc
     8401 acactccctt gctgtcgcag acgctgaatg atctgttctc gtcgctcgct ggtgcctgtc
     8461 attcgcttct cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg
     8521 tttcatttcg ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttaccc
     8581 attggcgcaa gctactctcg tttcagtggc tttcattatg tttcttttgt gaatcagatc
     8641 agaaaactat tatctttcgt tttattttta tctcaccatg gcgcagtatc aactgaaaca
     8701 aaacgaaaga ttaatatcgc agtaatctga actggagagg aaagtgaaac atctgacaga
     8761 aatggtgaga cagcacaaag cgggcaaaac aaatgggatt tatgccgttt gttccgcaca
     8821 tccgctggtg ctggaagctg caatccgcta cgccagtgca aaccaaacgc cgttactgat
     8881 tgaagcgacc tccaatcagg tggaccagtt cggcggttat accggaatga cgcccgccga
     8941 ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat ttcccacagg atgcgttgat
     9001 tctgggtggc gaccatctgg gtccaaatcg ctggcaaaac ctgcctgccg ctcaggcaat
     9061 ggccaatgcc gatgatttga ttaaaagcta cgtggcggca ggattcaaaa aaattcacct
     9121 tgattgcagc atgtcctgtc aggacgatcc ggttccctta actgatgaca tcgtggctga
     9181 acgcgccgca cgtctggcga aagtggcgga agaaacctgt cgtgaacact ttggcgaagc
     9241 cgatctggtg tatgtcattg gtaccgaagt accggtacct ggcggcgcgc atgaaacctt
     9301 aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca
     9361 cgcctttgaa aagcaaggtc tgagtgccat ctggccacgc atcattgctc tggtggttca
     9421 acccggcgtc gaattcgatc acaccaacgt tattgattat cagcccgtca aagcgaccgc
     9481 cttaagccag atggtcgaaa actacgaaac gctgattttc gaagcgcact ctaccgatta
     9541 ccaaacgccg caatcgttgc gccagctagt gattgaccac tttgccattc tgaaagttgg
     9601 cccggcgctg accttcgccc tgcgtgaagc tctgttctct ctggcagcga ttgaagaaga
     9661 actggtgcca gcaaaagcct gttctggtct gcgtcaggtg ctggaaaacg tgatgctcga
     9721 ccgcccggaa tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg
     9781 tggttatagc tactcggatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc
     9841 tttcgctcat ctggttcgta atctggcgga ttcaccaatt ccgctaccgc tcatcagcca
     9901 gtatttgccg ctgcaatacg tgaaagttcg ctccggcgag ctgcagccaa caccacggga
     9961 actcattatc aaccatattc aggacatcct ggcgcagtac cacacagcct gtgaaggcca
    10021 ataagcaaaa caaagaggaa cacgctatgc caaatattgt tttaagccgg attgatgagc
    10081 gtttgattca cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc
    10141 tggtagccaa cgatgaggtt gccgaagatc cggtacaaca aaacctgatg gaaatggtac
    10201 tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa agttatcgac aacatccacc
    10261 gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac acctgccgat ttcctgacgc
    10321 tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg
    10381 gcaagcaaca aatcgccaaa acggtttctg tggatgcggg cgatatcgca gcatttaacg
    10441 acctgaaagc cgctggggtg gaatgcttcg ttcagggcgt cccgacagag cctgctgtgg
    10501 acctctttaa attactttga gggattcatc atggaaatca gtctgttgca ggcattcgcg
    10561 ttgggcatta tcgcctttat cgctggcctg gatatgttta acggcttaac ccatatgcac
    10621 cgcccggtgg tcctcggccc gttagtcggg ctggtacttg gcgatctgca taccggtatt
    10681 ttaaccggcg gtacgctgga actggtgtgg atggggctgg ccccgctggc aggcgcacaa
    10741 ccgcctaacg tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg
    10801 aaacccgatg tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt
    10861 accttcctgt tctcggtgat gtctggcgta atgtcccgct gcgaccggat ggcggagaac
    10921 gccgatactc gcggcattga acgcgtgaac tatctggcac tgctggcact cggcactttc
    10981 tattttctct gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt
    11041 gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca
    11101 atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc ctacttcatc
    11161 ctgggctttg ttgccgcagc ctggctcaag ttaccggtgc tggctattgc tgccgccgcg
    11221 ctggcgatgg cgctgatcga cctgctgcgt aaatctcctg aaccgactca acctgcggca
    11281 cagaaagagg aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagtgaaaa
    11341 cgaagaaaca ctgctgactg gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc
    11401 agagctgaca aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc
    11461 ttttaactac gaacgtatgc aggcttccgg ctggttgtac ggtctgctgc ctgctctgaa
    11521 aaagatccac actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt
    11581 caatacccat ccgtttctgg tgacgtttgt tatcggcatt atccttgcga tggagcgttc
    11641 taagcaggac gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg
    11701 gattggcgat gcaatgttct ggctgacgct actgccgatt tgtggcggga ttggtgcaag
    11761 cctcgcgcta caaggctcta ttcttggcgc tgtcgtcttt attgtgctgt tcaacgtggt
    11821 acacctgggg ctgcgttttg gtctggcgca ttatgcttat cgcatgggcg tggcggcgat
    11881 tccactcatt aaagcgaata ccaaaaaagt cggccatgct gcgtcaatcg ttgggatgac
    11941 ggtaatcggc gcgctggtag caacctatgt tcgtttaagc accacgctgg aaatcaccgc
    12001 tggcgatgca gtggttaagt tacaggctga tgttatcgac aaactgatgc ctgccttctt
    12061 accgctggtc tacaccctga ccatgttctg gctggtacgc cgcggctgga gtccgctgcg
    12121 cctgattgca gttaccgtgg ttctcggtat cgtcggtaaa ttctgtcatt tcctttaaat
    12181 acaatgaggt attagatgtt aagtattatt ttgacagggc atggcggatt tgccagcggc
    12241 atggaaaaag caatgaagca aatcctcggc gagcaatcgc agtttatcgc catcgatttt
    12301 ccggaaacct caagcaccgc gctgcttaca tcgcagcttg aagaggcctt cgcccaactg
    12361 gattgtgaag acggcattgt ttttctgaca gatttgctgg gcggcacgcc gtttcgcgta
    12421 gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga tcaccggcac caatttgcaa
    12481 ctgctactgg agatggtgct ggagcgcgaa gggttaagcg gtgaagagtt ccgtgtgcag
    12541 gcgctggagt gtggacaccg tgggctgacc agtttggtgg acgagttagg ccgctgccat
    12601 gaagaatgtc cggtcgagga aggaatatga cacacgttct gcgcgccaga aggctgctga
    12661 ctgaagaggg atggctcgat gaccatcagt tgcgtattgc tgacggtgta atcgcagcaa
    12721 tcgaaccgat tccagtgggc gtgactgaac gcgatgcgga actgctctgc cccgcttaca
    12781 tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtgc
    12841 tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg cagttggtta ccgaccaccg
    12901 taaccgcgcc gcttaatacc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac
    12961 gcggcggacc tggtgcgcaa gtgctgggga gttatctcga aggaccatac ttcacgccgc
    13021 agaataaagg cgcgcatccg ccggagctgt ttcgcgagct ggaaattgcc gagctggatc
    13081 agttgattgc cgtttctcag cacaccttgc gcgtggtagc gctggcaccg gaaaaagaag
    13141 gggcattgca ggccatccgc catcttaaac agcaaaacgt acgggtgatg ctggggcata
    13201 gcgcggcgac ctggcaacaa acccgtgccg cgtttgacgc tggtgccgac ggcctggtgc
    13261 attgctataa cgggatgaca ggtttacatc accgcgaacc gggaatggtt ggcgcgggat
    13321 taacggacaa gcgcgcctgg ctggaactga tagccgatgg tcatcatgtg catccggcgg
    13381 cgatgtcgct gtgttgttgc tgtgcaaaag agagaatcgt gctcatcacc gacgcgatgc
    13441 aggcagcagg gatgccggat ggtcgctata cgttatgtgg tgaagaagtg cagatgcacg
    13501 gtggcgttgt ccgtaccgcg tccggtgggc tggcgggcag tacgctgtct gtcgatgcgg
    13561 cagtgcgcaa catggttgag ttggcgggtg taacgcctgc cgaagccatc catatggcgt
    13621 cgctgcatcc ggcgcaaatg ctgggtgttg atgatgttct gggatcgctt aaaccgggca
    13681 aacgcgccag catcgttgcg ctggatagcg ggctgcatgt gcaacaaatc tggattcagg
    13741 gtcaattagc ttcgttttga tggtttgctc ctttattggg ccttcacttc ccccgtaagg
    13801 cctttctttt tctttcgttg tgctctgtgc agggtatcgg atgcgacgct gacgcgtctt
    13861 ctttgaccta cggtacgccg catccggcaa tcggtgaaca tagcaacgtc atcacgccat
    13921 cctttaatct tcccttgtct tttctttcaa cgatcacaaa tttcgtttta tttctttttt
    13981 ctccattgaa ctttcagttt cttttctata gattttaatc aacgaaagac atcaccaagt
    14041 gaaatgaaac gaaaggtaag cgaaagcgac aacgcccgac gtcaagttca tcagactaag
    14101 gattgagtta tgccagaaaa ttacacccct gctgctgccg caaccggtac atggactgaa
    14161 gaagagatcc gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta
    14221 cgttccgcgc tcaataactt ccttgaaccg ttactgcgca aagagaatct gcgggtaatc
    14281 ctgaccggag ccggaacctc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc
    14341 catacaggta aaaacttcag cgccgtaccg accaccgatc tggtcaccaa tccgatggac
    14401 tacctgaatc cagctcatcc actgctgttg atctccttcg gtcgatccgg caacagcccg
    14461 gaaagcgtcg ccgccgtgga actggcaaat caatttgtac ccgagtgcta tcacctgccg
    14521 atcacctgca acgaagcggg cgctctttac caaaacgcga tcaacagcga caacgcgttt
    14581 gccctgctga tgcccgcaga aacgcacgac cgcggctttg cgatgaccag cagcattacc
    14641 accatgatgg tcagctgcct cgcggttttc gcacctgaga cgatcaacag ccagagcttc
    14701 cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt
    14761 gtgtttggtt acgcaccgtg gaaacggatc gtttatctcg gcagcggtgg cttacagggc
    14821 gcagcacgcg agtcggcgct gaaagtactg gaactgacgg cgggtaaact ggcggccttt
    14881 tatgattccc cgaccggatt ccgtcatggc ccgaaatcgc tggtcgataa cgaaacgctg
    14941 gtggtggtat ttgtctccag ccacccttac acccgtcagt atgatcttga tctactggct
    15001 gaacttcgcc gcgataacca ggcaatgcgc gtaatcgcca tcgccgcgga aagcacagac
    15061 atcgttgctg ccggtccaca tattatcctg ccgccgtccc gtcactttat cgacgttgag
    15121 caggcatttt gcttcctgat gtacgcccag acgtttgcac ttatgcagtc gctgcacatg
    15181 ggcaatacgc cggatacccc atcagccagc ggcaccgtta accgcgtggt gcaaggcgta
    15241 atcattcatc cgtggcaggc ataagaggat cgcattatga gcattatctc caccaaatat
    15301 ctgttacagg acgcccaggc caatggctac gcggtgcctg cttttaacat tcataacgcc
    15361 gagacgatcc aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc
    15421 ggaacgccgg ggacctttaa acatatcgcg ctggaagaga tctacgccct gtgtagtgcc
    15481 tattccacaa cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat
    15541 gatattcgcc gtaaagtcca cgcaggcgtg cgcagtgcga tgatcgacgg cagccacttc
    15601 ccgtttgacg agaacgtgaa gctggtgaaa tcggttgtcg acttctgcca ctcacaggat
    15661 tgcagcgtgg aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac
    15721 gccgaaagtg cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc
    15781 gtcgacagcc tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag
    15841 attgatttcc agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctgcat
    15901 ggtgccagcg atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa
    15961 gtgaacgttg ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg
    16021 gaaaatccgc agggtaatga tcctcgttat tatatgcgcg tcggtatgga tgcgatgaaa
    16081 gaagttgtca gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca
    16141 ttagtttttt atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg
    16201 gtaaaggaga taactaaacc ttaataaata catcagtaca atatcgcaac aataatatat
    16261 ttaaaaaaat tatattattc aactttatgg tgaggattac acaatgagca gtccaaatat
    16321 tctcttaacc cgtattgata accgtctggt tcacggtcag gttggcgtga cctggacatc
    16381 taccatcggt gcaaatctgc tggtagtcgt ggatgatgtt gtcgctaacg atgatattca
    16441 acagaaatta atgggtatta ccgcggaaac ctacggcttt ggcattcgtt tctttactat
    16501 cgaaaaaacc attaatgtca tcggcaaagc tgcaccgcat caaaagatct tcctgatttg
    16561 ccgtacgcca caaacggtac gtaaattggt agaaggtggc attgacctga aagatgtcaa
    16621 cgtcggcaat atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga
    16681 tgaccaggat ctcacggact tacgttttat taaacaacgt ggcgtgaatg ttttcattca
    16741 ggacgtccct ggcgatcaaa aagaacaaat ccctgactaa atctaaaatc gccttaatat
    16801 tggtttgagg taataaaaat gcatgaaata accctactcc aaggattatc cctggcggcg
    16861 ttagttttct ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata
    16921 atcgtttgta ccctaactgg cgctattctc ggtgatattc agactggctt aattaccggt
    16981 ggtctgacag agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac
    17041 ccgattatgg cgggtctgat gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa
    17101 acggcaattg gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc
    17161 tattccgctt tctcattatt tatgaccaaa gccgataaat gcgcgaaaga agcggatacg
    17221 gcagcgtttt cccgacttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg
    17281 attgctttcc tctgtactta cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg
    17341 cccgcctggc tgacccacgg ctttgaagtg gctggcggta ttctgcctgc cgttggtttt
    17401 ggcttgctgc tgcgcgtaat gttcaaagcg caatatatcc cttacctgat cgccggtttc
    17461 ctgtttgttt gctacatcca ggtcagcaac ctgttgccgg ttgccgtact gggcgcaggc
    17521 tttgcggtgt atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt
    17581 gccagtaaaa atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat
    17641 cacccgtctg ggctttcgtt cgtcactgct acaagcaagc tttaactacg aaaggatgca
    17701 ggcgggcggt tttacctggg cgatgttgcc gatcctgaaa aagatttata aggacgacaa
    17761 accgggccta agcgcggcga tgaaagataa cctcgaattt attaataccc acccgaatct
    17821 ggtcggattc ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac
    17881 cattaaaggc ctcaaagtgg cactgtttgg cccaatcgcc gggattggcg atgcgatttt
    17941 ctggtttacc ttattgccga ttatggcggg aatttgctca tcatttgcca gccagggaaa
    18001 cttgctgggg ccgattctat ttttcgccgt ttacctgctt atcttcttcc tgcgcgtcgg
    18061 ctggacccac gtcggttatt cagtcggcgt gaaggcgatc gataaagtgc gagagaactc
    18121 gcagatgatt gctcgttcgg caaccatcct cgggatcacg gtaatcggcg ggctgatcgc
    18181 ttcgtatgtg catattaacg tggtgacatc gtttgccatc gacagtaccc acagcgtcgc
    18241 gctgcagcag gatttcttcg ataaagtctt cccgaatatt ttaccgatgg cctacaccct
    18301 gctgatgtat tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac
    18361 gtttgttctt tctattgttt gttctgcatt cggcattttg taaatggaac gaggcaccgc
    18421 gtctggcggt gcctctttac ttaaggaatt tcatcctgtg caaacccttc aacaagttga
    18481 aaactatacg gcgttaagtg aacgtgccag cgaatattta ttggccgtga tccgtagcaa
    18541 accggatgcc gtgatttgcc tggcgaccgg agccacgcca ttactgacgt atcattatct
    18601 ggtagaaaaa atccaccagc agcaggttga tgtcagccag ctcaccttcg tgaagctcga
    18661 cgaatgggtg gatctgccat taacgatgcc tggcacctgc gaaactttcc tgcaacagca
    18721 tatcgtgcag ccgctggggc tacgtgaaga ccaactcatc agctttcgct ccgaagagat
    18781 aaatgagaca gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt
    18841 atgcgttctc ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaaacct
    18901 gcaaccggcc tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa
    18961 aaccgcgggc cgtcctgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc
    19021 ccgcgaagtt ttgttactgg tgactggcga aggaaagcag gatgcgacag aacgttttct
    19081 cacggctaaa gtctctaccg ctatcccggc ttcattttta tggctgcata accatttcac
    19141 atgcgtgatt gatgaaatgt gtcgtcgtta actcgccagg taaaggcgtt gtagcgctat
    19201 tcctgctgcg acgcctgtaa tcactctccc tgctgctcca gcgcatactt atacagcgca
    19261 tttttcttca cgccgtgaat ttctgcggcc agcgccgccg cttttttcag cggcagttct
    19321 gcctgtagca gcgccagcgt gcgcagggca tcagcaggca ggtcatcttc ctgcgcttta
    19381 tggccttcga caatcagtac catttcgcct ttgcgacggt tttcatcttc tttgacccac
    19441 gccagcagct cgccaacggg cgcgccgtga atggtttccc aggttttggt tagctcacgc
    19501 gccagaacca cgtagcggga ttcgcctaat accgcaacga tatcttccag gctatctaac
    19561 agacggtggg tagattcata aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc
    19621 gcatcacggc ggccttttga tttggcaggt aaaaagcctt cataacaaaa acgatcggat
    19681 ggtaaacccg cggcgcttaa cgcagtgata gcagcacacg gcccgggtag cggcaccacg
    19741 cgaatccccg cttcacggca ggtacgcacc agatggtagc caggatcgtt aattagcggc
    19801 gttccggcat cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt
    19861 tcggcttttt gttgttcgtt atggtcgtgc agcgcaaaca accgagcatt aatcccaaaa
    19921 tgttgcagca ataaaccggt gtgacgcgta tcctcggcgg caatcagatc aacggcctgt
    19981 aatacctcca acgcacgctg ggtgatatcc gccagattgc cgattggcgt cggtacaatg
    20041 taaagctggc cctgagaatt atccgccgat tggtgttgtt tcattgtgtc gtccgtattg
    20101 ccgatttaat attgagcatt gcgtaaaaaa aatatcactg gatacattat ggtaccctca
    20161 acattttctc gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc cctgattttc
    20221 gccggttgtg gcacccatac tcccgatcag tccactgctt atatgcaggg cacggcgcag
    20281 gctgattctg ccttttacct gcaacagatg cagcaaagtt ctgatgatac caggatcaac
    20341 tggcaattac tcgccattcg tgcactggtg aaagaaggta aaaccggaca ggcggtggag
    20401 ttgtttaacc aactaccgca agaactgaac gattctcagc gtcgcgagaa aacactgctg
    20461 gcggcagaga ttaaactggc gcagaaagat tttgctggcg cgcaaaactt gctggcgaaa
    20521 atcacacctg cagatttaga acaaaaccag caagcgcgtt actggcaggc aaaaatcgat
    20581 gccagccagg ggcgtccttc cattgattta ctgcgcgcgt taattgctca ggaaccgctg
    20641 ctcggtgcga aagaaaagca gcagaatatt gacgccacct ggcaggcgct ctcctccatg
    20701 actcaggaac aggcgaatac gctggtgatc aatgccgacg aaaatattct gcaaggctgg
    20761 ctggatctgc agcgcgtctg gtttgataac cgtaacgatc ccgacatgat gaaagccggg
    20821 atcgccgact ggcagaaacg ttatccgaac aacccgggcg cgaaaatgct gccaacgcag
    20881 ttggttaacg taaaagcgtt taaacccgca tcgaccaaca aaatcgccct gatgttgcca
    20941 ctgaatggcc aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa
    21001 aatatcggca ctcagccagt ggcggctcag gtagctgccg cacctgccgc agacgtagca
    21061 gaacaacctc agccgcaaac cgtggatggc gttgccagcc cggcacaagc ctcggttagc
    21121 gatctgaccg gtgaacaacc tgcggcccag ccggtgcctg taagcgcccc ggcggcaacc
    21181 accgcagcgg taagcgcacc cgcgaatcca tccgcagagc tgaaaatcta cgatacatca
    21241 tcacaaccac ttagccagat cttaagccag gttcaacagg atggcgcgag tattgtggtc
    21301 ggtccgttgc tgaaaaataa cgttgaagag ttgctgaaga gcaacacccc gctgaacgtg
    21361 ctggcactga accagccgga gaacatcgaa aaccgcgtca atatttgtta cttcgcgctt
    21421 tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg
    21481 ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg tagccaatgc gtttgcgcaa
    21541 gagtggcaga aactgggtgg cggcaccgtt ctgcaacaaa aatttggttc caccagcgaa
    21601 ttacgcgcgg gtgttaacgg cggttctggt attgctttaa cgggtagccc gattactccc
    21661 agagcgacaa ccgactccgg catgacgacc aacaatccaa cgctgcaaac cacgccaacc
    21721 gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt ggcaacgccg
    21781 ggtgaaatcg cttttatcaa accgatgatc gccatgcgta acggcagtca cagcggtgcg
    21841 acgctgtacg ccagctcccg cagtgcgcaa gggaccgctg gcccggattt ccgcctggag
    21901 atggaaggct tgcagtacag cgaaatcccg atgctggcag gcggtaatct gccgttaatg
    21961 cagcaggcgc tcagcgcggt gaacaacgat tattcgctgg ctcgcatgta tgcgatgggc
    22021 gttgatgcct ggtcgttggc aaatcatttc tcacaaatgc gccaggttca gggttttgaa
    22081 atcaacggta ataccggaag tctgacggct aacccggatt gcgtgattaa caggaagtta
    22141 tcatggctac agtaccaaca aggtcaggta gtccccgcca gttaaccacc aaacagaccg
    22201 gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg caaaggactg cggtttatcg
    22261 ccgctaacgt gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa ggccggacca
    22321 ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta tggcggcgcg gcagccagtg
    22381 tgacccgcag caaacaacac aaattattac agactgcccg cttgtggctc gcgcgtcata
    22441 atgggagttt tgatactgtg gattgccggt tcgatgtggt agccttcacc gggaatgagg
    22501 ttgagtggat taaggatgcc tttaatgacc actcataatt aaggtttaag gattagcgtg
    22561 caagaaagaa ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc ggcggcagag
    22621 gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg ttcagtctct gctcaatggc
    22681 aacaaaatcc tctgttgtgg taatggaact tccgctgcca atgcacagca ttttgctgcc
    22741 agcatgatca accgtttcga aacggagcgg cccagcttac ctgccattgc actaaatact
    22801 gataatgttg tcttaacggc gattgccaac gatcgcttac atgatgaagt gtatgcaaaa
    22861 caggtgcggg cgctgggtca tgcgggagat gtattgttag ccatttccac ccgtggcaac
    22921 agccgcgata ttgtaaaagc ggtagaagct gccgttacgc gtgatatgac cattgtggca
    22981 ttaaccggct atgacggcgg tgaacttgca ggtttgttag ggccacagga tgtggagatc
    23041 cgcattcctt cgcatcgtag tgctcgcatt caggagatgc atatgctgac ggtaaattgc
    23101 ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg atgtttaagg agaatacatg
    23161 aaggcattat cgccaatcgc agtccttctt tccgcgctgc tgttgcaagg ttgtgttgcc
    23221 gctgccgtag tgggtaccgc tgctgtgggt accaaagccg caactgatcc acgcagtgtc
    23281 ggcacccagg tggacgatgg taccctggaa gtgcgcgtaa acagcgcatt gtcgaaagac
    23341 gaacagatta agaaagaaac ccgtattaat gtcacggctt atcagggcaa agtgctgctg
    23401 gttgggcagt caccaaacgc tgaactttcg gctcgcgcta aacaaattgc gatgggcgtc
    23461 gacggtgcca acgaagtgta taacgagatt cgtcagggcc agccgattgg tctgggcgaa
    23521 gcgtctaacg atacgtggat caccaccaaa gtgcgttcgc agctgttaac cagcgacctg
    23581 gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct gatggggcta
    23641 gtgactgaac gtgaagcgaa agcggcggca gatattgcca gccgggtaag cggcgtgaag
    23701 cgggtaacca cagcgtttac gtttattaaa taagccattg atcggtcgtt gaagtaaata
    23761 ttcacacctt agccgacctg ttcccctcac cctaaccctc tccccaaagg ggagagggga
    23821 ccgtccagtg ctgtattcag gagcatccaa catcttaggc caacagcgcc aacccgccga
    23881 caatcacacc agatactgcc accatcgccc ccgtcagcca taaggctttc gccgggaacg
    23941 ctttacgcag cataatcagt gacggcaaac tcaccgccgg gagcgtcatc aacaatgcca
    24001 gcgccggagc ggttcccata cctgccagca tcatcgtttg cacaattgga atttctgctg
    24061 ccgtgggaat gacaaacaag catcctgcta ccgccatcgc caccacccac ataaggctgt
    24121 tatcgacagc accatcggca tgggggaata accagacgcg agcggcaccc aacaccagta
    24181 ctgcaaggat gtaaaccggg atcgtactcc agaaaagcgt ccatagcgcc ctgccccagc
    24241 ggctaaaaaa tccgccctgc gcttccggta tgtcaatttc gaccggtgcc tgcgttagcg
    24301 gtatttcacg tacccatttt tgtaccagcg tcgcaatcag caacaccatc gcaaggcctg
    24361 caaccagacg aatcgcggca aattgccaac cgaggacaaa ccccataaac accagcgtcg
    24421 ccgggtttaa caacggattg cccatccaga atgccagcgc cccgcccatc gatacctgtt
    24481 ggcgacgcat tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg
    24541 aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta cgcagcaacc
    24601 agtcacgcgg gatcagcacc tgtatcaacg aaccgaggat cacccccagc accgccgctt
    24661 tccataccgc gaggaaatag atcatcgcgt aatccaacgc cgcctgccac ggattagcat
    24721 ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc ggcagcagta aacgctttgc
    24781 cgtagtaagg ttgccatttc acataccaga gaccggcaat gacaaccaga aagaaaagcg
    24841 cgggtttcca ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat
    24901 tccccaggaa taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg
    24961 ggaataatta ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acaccttcat
    25021 gctcaggccg catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt
    25081 tccagttacc aggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt
    25141 tgctacgatc gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg
    25201 caattaatgc ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcct
    25261 tggcacccat agcactgact accagcatat gctgtgcgcc caaccgccgc ccggttaatg
    25321 cggtatccac taccagcgtg taatcggcat gaataaacgc ctctttactc cccgcttctc
    25381 gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg atcggtgacc tgcgccagcg
    25441 cgtcggtcag ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc
    25501 gcgtcggcgc ggctatggcg ttaactttcg gttcgttaat caacatccgc agcaggtgac
    25561 cgcccaccag ccccgttgcg cctgtaatca gtacctgact catcttcgct cctttacaga
    25621 attgtctgcc ttgcgctccc cggctcatgc accaggctta atagaccatg aggtaattat
    25681 ccccgattgt gggaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga
    25741 aaattgccgt tttaatcact gatgaatttg aggattcaga atttacttca cccgcagacg
    25801 agttccgtaa agccggacac gaagtgatta ccattgaaaa gcaagcgggt aaaacggtga
    25861 aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgccag
    25921 cggagtttga tgccctgctg ctaccgggcg gccattcacc ggattatctg cgtggcgaca
    25981 accgttttgt cactttcacc cgtgattttg tgaatagcgg caaaccagtt ttcgccatct
    26041 gtcacggccc gcagttgctg atcagtgccg atgtgattcg ggggcgcaaa ctgactgcag
    26101 ttaaaccgat cattattgat gttaaaaacg cgggcgcgga attttacgat caggaagtgg
    26161 tggtcgataa agatcagctg gttaccagcc gaacgccgga cgatctgcca gcgtttaacc
    26221 gcgaggcgtt acgcctgctc ggtgcctgag ttacgtagcc agatcatttt tttaccaaag
    26281 cccagcgtgt tgtcggtgaa tttgatttca tcaagccgga tttcccatac cggggccgac
    26341 agcattctgg caaccggaaa gcgacggttg tacgccttgc gtgcgaggtc gctttcctca
    26401 ccttcaagcc tgcggatctc gcctttaaat tgcacgccgc gaattaacgc taccgttttc
    26461 ggctgaccgt ttacagttcc ggcaactgcc gcctgcggcc cactcatctg cgcgtggcgc
    26521 gttttttctt ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaggca
    26581 ttggcgcacc acaattcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc
    26641 agccaacggc tgatggcggt gagtgtttcc attgctgttc tcttttatac tgtgggcctg
    26701 accttaacat gcgacgcaga cgatgacacc ctggtttctt tacttgatcc gtaccgccga
    26761 caataagctt tataccggga tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag
    26821 cggcaaaggg gcgaaagcac tgcgcgggaa aggagaacta acgctggcgt tttccgcgcc
    26881 agtgggcgat cgttcgctgg cgttacgggc agaatatcgc gttaagcaac tgaccaaacg
    26941 gcaaaaagaa cgtctggtag cggaaggcgc agggtttgca gaactgttga gcagcctgca
    27001 aaccccaaag gttaaaagcg attgaagtgc tcgtgatact caaccagacc ggttacgcca
    27061 ttcagcgcgt catccgctaa acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg
    27121 caacgcagat cgtgatgcgc cgccagctca aagccgaaac gactgtacag cgccggatcg
    27181 cccagcgtca ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc
    27241 agttggcgtg ccagcccttg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg
    27301 acccattgca ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc
    27361 tgaccttcgt catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg
    27421 tgaaccagct tcgcttccgc atcactttcg aatgaacgac gcagcagggc atcaataccc
    27481 ggcgcatcaa tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg
    27541 tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc
    27601 gtaaggcttt cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacagcc
    27661 ccagctccgt atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt
    27721 gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt
    27781 gcgcgttctg gctaacgacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa
    27841 tatcacgcac atgaatactt aaccagcggc cttcaagaaa ctccagctcg ccatcatcca
    27901 gcgcctgacg gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg
    27961 gcgtcagttt taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca
    28021 gtttatccaa cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga
    28081 aaaataacat agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca
    28141 actttaactg ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt
    28201 aacatttttg cgctttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg
    28261 ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg
    28321 ggctaaaaga tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat
    28381 tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca
    28441 cttttgcgca cccggacggt tacgcccgct ggcagcgcgc cgtggatatg gcggcgcagc
    28501 tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt
    28561 acccgcatat tgaacgtcat gtgtcagtgc aggcttcggc gactaatgaa gaggcgattc
    28621 acttttatca tcgccatttt gacgttgcac gcgtggtgct gccgcgcgtg ttgtcgattc
    28681 atcaggtgaa acaactagca cgggtcacac ctgtaccact ggaagtcttt gctttcggca
    28741 gcctgtgcat tatgtcggaa ggtcgttgct atctgtcgtc gtatctgacg ggtgagtcgc
    28801 ccaacaccgt gggcgcgtgt tctccggccc gtttcgtgcg ctggcagcaa acgccgcagg
    28861 ggctggaatc tcgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag
    28921 gttatccgac gctgtgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg
    28981 aagaaccaac cagtctcaat acccttgagc tactgccgga gttaatggcg gcgaatatcg
    29041 cttcggtgaa aattgaaggc cgccagcgta gcccggcgta tgtcagccag gtggcaaaag
    29101 tctggcgtca ggctatcgac cgttgtaagg ccgatccgca aaacttcgta ccacaaagcg
    29161 cgtggatgga gacgctcggg tcgatgtccg aaggcacgca gaccaccctt ggcgcgtatc
    29221 accgtaaatg gcagtgagaa gagcaatgaa atattcctta gggccggtgc tatggtactg
    29281 gccaaaagag acgctggaag atttttatca gcaggccgcc gccagcaacg cagacgtgat
    29341 ttatctcggt gaagcggtct gtagcaaacg ccgggcaacc aaagttggcg actggctgga
    29401 gatggcgaaa tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcactggt
    29461 gcaggcgtcc tccgaactgg gcgaactgaa acgctatgtt gagaacggtg agtttctgat
    29521 tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc
    29581 cgggcacgcg ctgaactgct acaacgcggt gaccctgaaa atattgctca aacagggcat
    29641 gatgcgctgg tgtatgccgg tggagctatc ccgcgactgg ctggtgaatc tgcttaatca
    29701 gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa gtcttgagct acggtcatct
    29761 gccgctggcc tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga
    29821 gtgcgaaacc tgctgcatta agtatccgaa cgggcgcaac gttctgtcgc aggaaaacca
    29881 gcaagtgttt gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa
    29941 cgagctggca tccatgcagg ggctggtcga tgtggtccgc ctgtcaccgc aggggactga
    30001 cactttcact atgctcgacg ccttccgcgc caatgaaaat ggcgctgcgc cactgccgtt
    30061 gacggcaaac agcgattgta acggctactg gcggcggctg gccgggctgg agctgcaagc
    30121 gtaagtaaat agctcacttt gttaacaact ttaactactc tttaatgcag tattaaagat
    30181 taatcggtaa caaagtgagc tgttatgact gataaaacca ttgcgttttc gctactcgat
    30241 ctggccccca ttcccgaagg ttcttcagcg cgagaagcgt tctcccactc tctcgatctc
    30301 gcccgtctgg ctgaaaagcg cggctatcat cgctactggc tggcagaaca ccacaatatg
    30361 accggcattg ccagtgctgc aacatcagta ttaattggct atctggcggc gaataccacc
    30421 acgctgcatc tgggttccgg cggcgtgatg ttaccaaacc actcaccgtt ggtcattgcc
    30481 gaacagttcg gcacgctcaa tacgctctat ccggggcgaa tcgatttggg gctgggtcgt
    30541 gctccgggta gtgaccagcg aaccatgatg gctctacgtc gtcatatgag cggcgatatt
    30601 gataatttcc cccgcgatgt cgcggagctg gtggactggt ttgacgcccg cgatcccaat
    30661 ccgcatgtgc gcccggtacc gggctatggc gagaaaatcc ccgtgtggtt gttaggctcc
    30721 agcctttaca gcgcgcaact ggcggcgcag cttggtctgc cgtttgcgtt tgcctcacac
    30781 ttcgcgccgg atatgttgtt ccaggcgctg catctttatc gcagcaac
//
LOCUS       DAYPHN010000041        30531 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000041 DAYPHN010000000
VERSION     DAYPHN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.041"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..321
                     /locus_tag="ACG0BW_001821"
     CDS             <1..321
                     /locus_tag="ACG0BW_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFK7258792.1"
                     /translation="NMGGEWHRPLIWGFFAFYGVWAALSLEGKIKPAKLIIFLGDASY
                     SIYLTHGLITAWVSYMFVSFGLIDYSNVFLYIGIYFIISCALGSVIHMYIEKPIAEFL
                     KRFV"
     gene            complement(550..1137)
                     /locus_tag="ACG0BW_001822"
     CDS             complement(550..1137)
                     /locus_tag="ACG0BW_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFK7258793.1"
                     /translation="MKPVFDENGLATQAGDIRCFYYDAVTMEYAGWSDEYINAGVSMP
                     GNSTDVDPGEMVDGKVFMFTGTGWRQDEDHRGETVYSTDDTSSSTIDYIGPVRKGYTT
                     VAPSGPYQKWDGKKWVTDTDAQHVADVEAAEQKKAALLAEAQDKISLWQTELRLDIIS
                     DGDKESLIAWVQYIKAIQAIDTSFAPNINWPTEPE"
     gene            complement(1917..2219)
                     /gene="stfP"
                     /locus_tag="ACG0BW_001823"
                     /pseudo
     CDS             complement(1917..2219)
                     /gene="stfP"
                     /locus_tag="ACG0BW_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein StfP"
     gene            complement(2223..2807)
                     /gene="ymfQ"
                     /locus_tag="ACG0BW_001824"
     CDS             complement(2223..2807)
                     /gene="ymfQ"
                     /locus_tag="ACG0BW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFK7258794.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINENFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(2798..3856)
                     /locus_tag="ACG0BW_001825"
     CDS             complement(2798..3856)
                     /locus_tag="ACG0BW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFK7258795.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSNAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            complement(3843..4268)
                     /locus_tag="ACG0BW_001826"
     CDS             complement(3843..4268)
                     /locus_tag="ACG0BW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFK7258796.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVLTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(4268..4816)
                     /locus_tag="ACG0BW_001827"
     CDS             complement(4268..4816)
                     /locus_tag="ACG0BW_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFK7258797.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNSPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(4816..5895)
                     /locus_tag="ACG0BW_001828"
     CDS             complement(4816..5895)
                     /locus_tag="ACG0BW_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFK7258798.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDTHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(5892..7220)
                     /locus_tag="ACG0BW_001829"
     CDS             complement(5892..7220)
                     /locus_tag="ACG0BW_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFK7258799.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DQGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNVSTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(7281..9116)
                     /locus_tag="ACG0BW_001830"
     CDS             complement(7281..9116)
                     /locus_tag="ACG0BW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFK7258800.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADKNPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(9258..9527)
                     /locus_tag="ACG0BW_001831"
     CDS             complement(9258..9527)
                     /locus_tag="ACG0BW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFK7258801.1"
                     /translation="MKELELKKPIIAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(9527..9883)
                     /locus_tag="ACG0BW_001832"
     CDS             complement(9527..9883)
                     /locus_tag="ACG0BW_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFK7258802.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(9883..11379)
                     /locus_tag="ACG0BW_001833"
     CDS             complement(9883..11379)
                     /locus_tag="ACG0BW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFK7258803.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVATIASSIQDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYSAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(11363..11533)
                     /locus_tag="ACG0BW_001834"
     CDS             complement(11363..11533)
                     /locus_tag="ACG0BW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFK7258804.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(11542..12102)
                     /locus_tag="ACG0BW_001835"
     CDS             complement(11542..12102)
                     /locus_tag="ACG0BW_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258805.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEECGNPINYDGGTLLDLNRHELIYQFDFSIISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            complement(12099..12605)
                     /locus_tag="ACG0BW_001836"
     CDS             complement(12099..12605)
                     /locus_tag="ACG0BW_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258806.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(12580..12990)
                     /locus_tag="ACG0BW_001837"
     CDS             complement(12580..12990)
                     /locus_tag="ACG0BW_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFK7258807.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFRTWAKVVQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            complement(12987..13310)
                     /locus_tag="ACG0BW_001838"
     CDS             complement(12987..13310)
                     /locus_tag="ACG0BW_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFK7258808.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTDVEKMELPMSFNWLVGPY
                     RYFPQ"
     gene            complement(13313..13513)
                     /locus_tag="ACG0BW_001839"
     CDS             complement(13313..13513)
                     /locus_tag="ACG0BW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258809.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(13563..14768)
                     /locus_tag="ACG0BW_001840"
     CDS             complement(13563..14768)
                     /locus_tag="ACG0BW_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFK7258810.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(14783..15433)
                     /locus_tag="ACG0BW_001841"
     CDS             complement(14783..15433)
                     /locus_tag="ACG0BW_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFK7258811.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(15411..16652)
                     /locus_tag="ACG0BW_001842"
     CDS             complement(15411..16652)
                     /locus_tag="ACG0BW_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFK7258812.1"
                     /translation="MFFSGLFQRKSDAPVTTSAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNSYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGNVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(16652..16834)
                     /gene="ymfR"
                     /locus_tag="ACG0BW_001843"
     CDS             complement(16652..16834)
                     /gene="ymfR"
                     /locus_tag="ACG0BW_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFK7258813.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQMSVGGGK"
     gene            complement(16846..18579)
                     /locus_tag="ACG0BW_001844"
     CDS             complement(16846..18579)
                     /locus_tag="ACG0BW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFK7258814.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KNKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLMEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTNPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGILTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIFQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(18576..19070)
                     /locus_tag="ACG0BW_001845"
     CDS             complement(18576..19070)
                     /locus_tag="ACG0BW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFK7258815.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(19195..19545)
                     /locus_tag="ACG0BW_001846"
     CDS             complement(19195..19545)
                     /locus_tag="ACG0BW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFK7258816.1"
                     /translation="MPPRTPKACRVRGCRNTTTDPSGYCESHKSEGWKQYKPGLSRHQ
                     RGYGSKWDTIRERVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            complement(19964..20221)
                     /locus_tag="ACG0BW_001847"
     CDS             complement(19964..20221)
                     /locus_tag="ACG0BW_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFK7258817.1"
                     /translation="MMIVLHVMCLLPLLTGCGSTRTVYVPVPAVPLPTSLTTETPQPV
                     IPDPLTYGASLDLNVSLLSALGQCNIDKASIRKIETSRNSQ"
     gene            complement(20106..20498)
                     /locus_tag="ACG0BW_001848"
     CDS             complement(20106..20498)
                     /locus_tag="ACG0BW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 family protein"
                     /protein_id="HFK7258818.1"
                     /translation="MKLRYKLVIVAFVVSVIGSFIWSAGHYYSKYQHEKERADEAVRN
                     AESATAITRNVLQSLQIINTVIEANQHAKQQIALESQRTQEDIKVAVADDDCASRHVP
                     AAAADRLRKYANSLRAGSGGTAANKPDH"
     gene            complement(20482..20958)
                     /locus_tag="ACG0BW_001849"
     CDS             complement(20482..20958)
                     /locus_tag="ACG0BW_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFK7258819.1"
                     /translation="MQNLNPQRKAFLDMVAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDSYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDICQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKKAGGVVNEAEI"
     gene            complement(20945..21250)
                     /locus_tag="ACG0BW_001850"
     CDS             complement(20945..21250)
                     /locus_tag="ACG0BW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFK7258820.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(21572..22261)
                     /locus_tag="ACG0BW_001851"
     CDS             complement(21572..22261)
                     /locus_tag="ACG0BW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFK7258821.1"
                     /translation="MNIQYLQYVREQLMVATADLSGVTKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVAITQWAWNEFKALLGTRKIASKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQSIAKLD"
     gene            complement(22258..22398)
                     /locus_tag="ACG0BW_001852"
     CDS             complement(22258..22398)
                     /locus_tag="ACG0BW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFK7258822.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(22395..22757)
                     /gene="rusA"
                     /locus_tag="ACG0BW_001853"
     CDS             complement(22395..22757)
                     /gene="rusA"
                     /locus_tag="ACG0BW_001853"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFK7258823.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(22754..23044)
                     /gene="ybcO"
                     /locus_tag="ACG0BW_001854"
     CDS             complement(22754..23044)
                     /gene="ybcO"
                     /locus_tag="ACG0BW_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFK7258824.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(23037..23207)
                     /gene="ninE"
                     /locus_tag="ACG0BW_001855"
     CDS             complement(23037..23207)
                     /gene="ninE"
                     /locus_tag="ACG0BW_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFK7258825.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(23207..23662)
                     /gene="ybcN"
                     /locus_tag="ACG0BW_001856"
     CDS             complement(23207..23662)
                     /gene="ybcN"
                     /locus_tag="ACG0BW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFK7258826.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEEGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPPSCEFQLLRDKQEA"
     gene            complement(23659..23760)
                     /locus_tag="ACG0BW_001857"
     CDS             complement(23659..23760)
                     /locus_tag="ACG0BW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258827.1"
                     /translation="MRTYNLNSLLLSQMQKCTCDFLHPAFDLCGGEA"
     gene            complement(23852..24334)
                     /locus_tag="ACG0BW_001858"
     CDS             complement(23852..24334)
                     /locus_tag="ACG0BW_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258828.1"
                     /translation="MISAEGLQRLLNLSEPPSDELIDVYLQFCMIFPRVEQSFFGGFA
                     KGEDSLNYARTLLDAGIKVPNELFHDFKQRYILNGDAQERLDALCVGRERDRRGIVNG
                     LQNDAATDLNTMSTVLKIVIRIRNNLFHGNKDAYLFVDSQEQAQLLRWCVRFLQGLLN
                     "
     gene            complement(24331..24579)
                     /locus_tag="ACG0BW_001859"
                     /pseudo
     CDS             complement(24331..24579)
                     /locus_tag="ACG0BW_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693515.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
     gene            complement(24591..25343)
                     /locus_tag="ACG0BW_001860"
     CDS             complement(24591..25343)
                     /locus_tag="ACG0BW_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFK7258829.1"
                     /translation="MDPKLLYATFKRNDAPAWRCPNCMNETLEIVADSFVETDSSATT
                     QFRDEVWFDEEMSGGVFSCVLRCTRQACQENVALSGQVVAVECFNDEMTERWYVSGFR
                     PKYFYPPLPLFLFPEKCPEDIADLLAEVSALIPSHPASAVNTMRTILEMMLDSLDVPR
                     EKTVKGKIIRLSTHERITNYSDKLGSNKDAFMALKWLGNHGSHGGIKVTRSSINDACI
                     LIGHLIDFLFLESPDVTIHIERINNVYAPKKE"
     gene            complement(25618..25926)
                     /locus_tag="ACG0BW_001861"
     CDS             complement(25618..25926)
                     /locus_tag="ACG0BW_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFK7258830.1"
                     /translation="MTGKEAILHYLKTHRTFCAPDVAAATGVTVTSINQAAAKMARAG
                     ILVVDGKVWRKVYYRFATREEREGKVSTNLIFKECRQSAVMKRVLMFLGGGRGISETL
                     "
     gene            complement(25923..26624)
                     /locus_tag="ACG0BW_001862"
     CDS             complement(25923..26624)
                     /locus_tag="ACG0BW_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFK7258831.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(26621..27640)
                     /locus_tag="ACG0BW_001863"
     CDS             complement(26621..27640)
                     /locus_tag="ACG0BW_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFK7258832.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     EAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKQDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            complement(27637..28176)
                     /locus_tag="ACG0BW_001864"
     CDS             complement(27637..28176)
                     /locus_tag="ACG0BW_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFK7258833.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSYMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(28207..28434)
                     /locus_tag="ACG0BW_001865"
     CDS             complement(28207..28434)
                     /locus_tag="ACG0BW_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7258834.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            28545..29237
                     /locus_tag="ACG0BW_001866"
     CDS             28545..29237
                     /locus_tag="ACG0BW_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFK7258835.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVSHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            29311..30132
                     /locus_tag="ACG0BW_001867"
     CDS             29311..30132
                     /locus_tag="ACG0BW_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258836.1"
                     /translation="MTKEQEPNTDEVKPEQGSLFELEFHEYTPSSLGTAHLSGFAIAT
                     DEMEYAVKSMRSTPPFPVQNPTQVPAAEWFCTNLAEKCGIATPVCKILKCIANGEYVF
                     GSRIEFSAWKSGLNSPQWINLLSNASESLKKQLWAIYAFDQFVYNIDRHLNNYLYMEN
                     TRGNVIVKTFDFSLSSFVIGWPRNTPHILPTDSSTTTNWTIAKQFIGDTDDLRKCALS
                     VLDKIEKIGVETISDILNSMPEAWMPPMHKEYFLKWWDSEERIDRLNAIRLEITS"
     gene            30129..>30531
                     /locus_tag="ACG0BW_001868"
     CDS             30129..>30531
                     /locus_tag="ACG0BW_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFK7258837.1"
                     /translation="MKTFKYSLIRVTPNLEKGETINVGLIVYHDSDIDVRMLNSVSKL
                     KAIDKDLTLDYLEDLSTSLFDLSQKINNAELLPCLFKGALSLSSFGMFTLQANEDYEA
                     KVTDLMNRLVNPQKQPHKQLKKKVFSELKSTF"
BASE COUNT         7040 a         8505 c         7321 g         7665 t
ORIGIN      
        1 aacatggggg gggaatggca tcgtcctttg atatgggggt ttttcgcttt ttatggtgtc
       61 tgggcagcgt tgagccttga aggtaaaata aaaccggcta aacttattat tttcctcggt
      121 gatgcttcat atagtatata tttgacgcat ggtttgatta cagcttgggt atcatatatg
      181 tttgtgtctt tcggacttat tgattattcc aatgtatttc tatatattgg aatatacttt
      241 ataataagtt gtgcgcttgg cagcgtcatt catatgtaca tagaaaaacc catcgcagaa
      301 tttttaaaac gttttgtttg accaaatggt cataactacc ccccttaaaa tacgaaagta
      361 aataattaga acatgtgctt tgagttaaac actcaaagca caaaataaaa agcgctgcca
      421 ttgtctattt tgccccttct cacgctcgtt ttttatttta taataataca cctccagtta
      481 cactgttgta attaaaagca tgaatcatca attagttcat cccttaatct aactcttccc
      541 caaacgttcc tattctggtt cggtgggcca gttgatattt ggcgcaaatg aagtatctat
      601 agcctgaatg gcttttatgt actgcaccca cgcaatcaga ctttccttat ccccatcact
      661 gatgatgtcc agtcgcaatt ctgtctgcca gagacttatt ttatcctgcg cttcagccag
      721 taacgcagcc ttcttctgtt cagcggcttc aacatcggca acgtgctgtg cgtcagtatc
      781 tgttacccac tttttgccgt cccacttttg atatggtcct gatggcgcaa cagttgtgta
      841 ccccttcctg actgggccga tataatctat agtggaagat gaggtatcat cggttgaata
      901 taccgtttca ccacgatgat cttcatcctg tcgccatccg gtgcccgtga acataaaaac
      961 ctttccatca accatttcac ctggatcaac atctgttgaa ttacccggca tgctgacgcc
     1021 agcgttaata tattcatctg accagccagc atattccatg gtcactgcat cgtaataaaa
     1081 acaacgaata tcaccggcct gcgttgccag accattttca tcaaagacag gtttcattac
     1141 tttgccctca ccaggaaatt gaatgcgatg ttgcgcggtc ggttctcgtt tgcaattgat
     1201 accgatgggt cttttccagc gctgaagaga atactttgag caacagtgct tccgcctggc
     1261 tggaggttcg ttaagttttg tgccccccca gaataaagcg caccagaatt tgtagtattc
     1321 ccaaaatatt gaacaaaacc gacttcacca gtgatatttc ggatggcatc cacctgagta
     1381 ctcagcaatg cccttcctga atcgacccca cgaccatcat cccagatacg cgggaattcg
     1441 cctcgagctt cgggaagtgt aagtccaggg aacacctgtg caagtttggg gtataccgca
     1501 gcagaaaatg acgcgccgtt aaattttaaa aacaccatgt cagaccattc gcttatgacc
     1561 gtattcggca ttgctgcgga gggccagaag aatgggacac cgatagctgg cgcaccagac
     1621 cccatcccca catctgctgg cgttggctta tatttttcag aaaagagctt atacttccaa
     1681 atcgtagtgt tgccggttac agcacgaatg attggtgttg ctccagcaga atttgctagc
     1741 ggaagataaa tctgcactcg ttgttttgat tcggaatcat acaacaacgt tagcaccatt
     1801 ccgtaatcaa tcccaaaatc cgggtctgta ttaccgccgt atgcataaaa accagaactt
     1861 gttgcgctcg aaagcaagtt aatgttagaa ccagcgatat ttttaccgat acccaggtcc
     1921 gttaaatcaa ggttttcgag agctgttttc actgtgccat ccgatttgat atcaccaaac
     1981 ggattcttgc ggcttaacag cagagcacga agcgcggtaa gcaactggtc gtttcgcccc
     2041 ttctccaggc tggcaccgga tgcctccacc acgctgcaga gttcttcctg caacatatca
     2101 aagtaatcat catccagatc ggtggcaggt gtgccagtct gggggttacc acgggtaaaa
     2161 ccgttcttac ccgcgccgaa cttatccttc tgcgcggttt tcgtgtctat acgatgcatg
     2221 gattactccg gatatttaaa aattacgtag gtatgcgacg ggcagagttt gttaagcaca
     2281 cattcgacaa cggtgtcgcc ccagatacgc agcgcggaat cacagggatc gccacatgtc
     2341 atccaggtgg tgtttgtggc ggctggcatg ttgacctgcc agtaataccg ccattccggc
     2401 gcattcaccg cgtcagtaca ggccgatgag caggtgaacg tgcttttgtc gtatcgcgtg
     2461 atggtagcgt ctggtctgcc cagggcagca agctgtgcaa gataaaaatt ctcgttgatg
     2521 ccgcccgcca ggttaacctt cgcatccagc cgctgctgac gctgacgaag ggtctgtgtc
     2581 cctgcgggaa tacattcatc cggcagaccg cacagacgct cccagcggtt tatcagttca
     2641 gtggtggtgc gcggatccag ctcccgcatc agggcatccg cacgctgatg aacgcgggtt
     2701 aatgacggtg ccgcaccggc aatcgccgga tcgctggctg accacgccgg accggggggc
     2761 aacagtgccg acaacagacg gatgtaatca tcgtttgtca cgtccatgaa atcgtcccca
     2821 gtaccgccag ttcatttttt gcaatggaga tattgtctgc cggtgcaagc aactgatggc
     2881 tgtattcccc gttcgcaccg gaaatcgcct cactgatacg cgataccttc agttctccct
     2941 gcggataacc atcacgcagc aggaacgaac gcaactccgc ggtgatggca gcccgtattt
     3001 ccggtgtgtc cggcgtcacg cggatatgaa aatccaccgt atgtgccacc ggcctgaaca
     3061 catacaaatc agaacctgcc accggggcca gtggcccgat atgttgtctt gccgccgttt
     3121 ccgttgattc ttcaggaatg ggattaatca ggtcactgct ggcaatcatc acaccgacag
     3181 ttcccgttcc catccagtga cggtatgtcc atgcgcgggt aatgccgggc acttctttag
     3241 cccagacgac atagtccccg tcagccccgc cctgcggcgt ccagtaatac cgctcaatga
     3301 cgcgggcgcg ccacgtttcc agctcttcag tatcaaatcc gcctgtcagg gtgtcagcca
     3361 caccggaaga cggcagacca ttcaccggcg tgaccaggat taatgccgta ccgtcgtcag
     3421 cgttaccgac cgcgcctgca tttgagcagg cgatcggcac gcgcaggaca ccaccggagc
     3481 tggttgcatc ggcagttgca gtatactgaa ccaggtcatc gcgctgaata acactcccgg
     3541 cggtcacctt caggccatcg ctgacacctt cccagcgcat atacccgctg gcagccgtgg
     3601 cccccttgcg cggacaccgt ttcatcgcag catgtcgcgc cagccaggac tcatcgcaca
     3661 ggtcaggcag catgttcatt gccagataat cgatgtaccc gtaaaccgta tgcagcgccg
     3721 ccgcatacac ctttgcccgc acgtcttcat ccatgcgccg gagcgtgtcg ctgacgtcca
     3781 gcctggcgaa taaatcgtta cggagcatac tgatattttc tgccagcgtc gggcgctgaa
     3841 attcactgtc cgccatgcgt tatcgcactc cacagatcat caaaagaaat cattaccggt
     3901 ccgtcacgac gccagagagt gatactgtta cccagttcat taatcccggt gcggcggata
     3961 tccagatcaa tacgggacac cacgccgtca tcaatcatcc attgcaggca ttcgcggata
     4021 taccccctta ccgtcagcac cagctgattg gtcagtttgc tgcgctgaag cagccacagc
     4081 cgggagccgt aacggtcatt ctgtaccgca ggccaggtat ccccccacca tcccatcggg
     4141 acgtcggcgt tgtcatcagg ctccgcccgc cgccaggtaa acagggaaat caccacggcg
     4201 cgggtcagcg gatccagcgg tgcgctggcg caggtgcgtt taccgttcac cgtcagccac
     4261 agttccatca tgcctccatc gctttatcag gtttgtcggt gttactgccc tgaccgttct
     4321 ctctgtgacg atgcccgtta taggcaagcc gcatcgctga catggtggtg ccgccggagt
     4381 cgcacaggtc tttcacctgt cctgtcactt ccaggtccat ttcaaaacgt gctttaggtg
     4441 aattgcgaaa cgtgatcgtt ttacctgcac cgtccaccac gatcccctcc cgggtcagcg
     4501 tcacggactg cccctgatcg tcatagacag ccacctcacc cgtctgcagc cctttcaggc
     4561 ggtagcgccg gtccgacacc gtaacaacca ccgcatgaga acggtcacca tccggaaaca
     4621 acaccaccgc ttccgcaccg ctgtttgccc ttgcggtaaa accgtagggt tcaagatgtt
     4681 caaccccggc tttgggttca ccggcaatca gggacacatc cacggtctga catttcgtgg
     4741 cggcactgat acttttcacc actgcccgcc caatcaggcc gaggagttgt cgctgcatgg
     4801 cttcaatcgt cctcatcaga acgggtcctc ctgtactctg gcttttttct ttttccgcgc
     4861 gccgggggct tcgggttcag gcagataagc atcaggtggg ccgacacgga tttccgtcag
     4921 ggtgccgttc tggtcctgag taaacgtgac ttccgagaca agcagttcgg tattgtcgaa
     4981 accacagacc ggatcgaaga caatcacccg ctggttgggc tgccacagcg taccattacc
     5041 ctgtcgccag ccctgcacca cataggtggt ttcatccgtc cgcgccgccc gttgtcgggc
     5101 ttcaaagtca gcacgcgcaa tacagcctgc ccccgtagcc tgccctgtct gcctgatata
     5161 catcggacgg taacgggcaa taaatgcgtc ctctgtgcgg gcccgcagcg cggtggtggt
     5221 ggcctcaccg aaatcatcgt cgtttccggc acgctgcccc gccacctggt aaacagaaaa
     5281 ccgctcccgg atactcttct ccgtatcgca ggaaaggatg ttttccccaa gtaccagcgc
     5341 ggtatgtgcc cgcgttgagc caataccgcc aatcaccagc ctgccgtgcg tgtcgtcata
     5401 agccagcgcc tgctgctgac cgagtatttt gttaatcacc tcgatcaccg tttcaccgtg
     5461 atcaggctgg acatcaggaa taacacccga cggcgcaccg ctgttcacca cctcaatgcc
     5521 gaagggcgca gcaagcgcct gcgcaatctg taccagtgat cgtccgttaa actgtgtcgg
     5581 ttcggctgca cagtcaatca ggtcagcggt cagactgcgt ccggcaatac cggtgctgac
     5641 cgaacgggca tcgtaacgaa cgggcgtcgc ctccacccag ccggtgatca ccagctcatc
     5701 accaatcagc acttccactt ttgaaccgtt tttaatgcgc ggctgaagcg tggtgatacc
     5761 ctcatctccc ggccactggc gagtgatctc cacactgaaa tcccgcgcca gccgttcaat
     5821 accggcaccg atgcgcaccg atgtccagcc attccactcc cggccattta cccgtagcgt
     5881 gacattgtcg ttcattgcac tggcaccttc agagggatca ccggcacaaa gccgggatgc
     5941 gtaatggcat tacgccggat aatgtccgcg tcacgcgccg cgttatcaaa ccaggtcgca
     6001 gccagcacca gcgcgggtaa aacctcatcc ggcgtgcgct gaatgatccg tgcagactgt
     6061 tcaaggcgcg tgttgatatc cgcattcaga tctgctttca cccggcgcag cgccagaaac
     6121 agcgcatcac tggttgtacg ggacaactcc ttatcaattg ccgtattcag tgtgtcgcga
     6181 atgtcggtca gttcttccca cgttggcagg tcaaccgtgt ttttcaccgc cggtgcattg
     6241 ttcagtgccg gatgcgtgac agaaggccag ccggtgctct gcgcgggtgt tgttgactgc
     6301 cccactgtgg cattctgcat caccgcggaa gttgtgggcg caggcaatcg tgtgacggca
     6361 tacgccgctt cgctgattgc ggtcgtacga agggtgctgg caaccacgtt acgctgctgc
     6421 gtcgccgtgg cggtggtttt actgtccgtt ttccagacgc cgcgcggttg cagatcgctg
     6481 ccgaggctga caccggaaag cgttttgatc atggtgacca ggtcgctggc gttaccataa
     6541 aggcgtttcc cggtacgcca cattttctgc acctgctcaa cgaaattttt gcctgacgat
     6601 ggcggcggca gaagtaccga gatatccccc tgcaacagcc tggcggcatc cgatacggca
     6661 gaatccacca ctttcatcgc atcagaaaca tacccaagca ttgtgctgac attaccgacg
     6721 acgtcgttct gcacaaaatc cgccacgcca tcgatactga aaccgctgaa gctgtcactg
     6781 atgcagtcat ccagtgcaga acaggatgac atcagcgtct gcgccgtcgc cgcacctgat
     6841 gtggggtaag agagttctcc tgcttcgaca aacttcaggt caaaacggac aatacgccct
     6901 tcactcttcg atgtgctgac ccgaacttcc ccgtcaacac agactttcag ctcaccgtaa
     6961 gtcggatgga caagcgtgcc gggaccgggt ttattcagag cgtcaatcag gcgatcgcgc
     7021 tggtcaaagc agtcatctcc caccacataa gccgtgatgg acgggcggaa agtgattttc
     7081 ccctggtcct cggtataggg tttgtcgcgg ttcgggtatt cgtgcgtttc cacacgacgg
     7141 ccggttcccg cactttcttc ttcaacctta aacggcacac cgcgaaatga cgcgtcctga
     7201 agtctgtctt tccacgtcat ataaactccg tacataaaaa atcccaccgg agtgggactc
     7261 attaacagat taatttttca ttacctgcca aagcgcgtat agccaacatc atggctgaca
     7321 tcaaaaccgc tggatcgcgt ttccataacc cgcatacccg gaggcgaatt cacaaaagag
     7381 accttgatct ccccatcaac ttttggcgca gaagctttgt taatcatgaa gggattcggg
     7441 cctgtggcat cggaggcgtt gtttgactga gccagatcca ccgccggata aggtgtgtat
     7501 ccccgcgccg gtattcccgt cccataagca tcataagcac ccgcgcccca ctgcgcagag
     7561 ttaatggcat cgaccgtgtc accggaactg tcggtaaacc actcaataat tggcttcagc
     7621 ttgtcccaca tatcctgaaa ccacttaaca accggccccc agttattgat caccatcccc
     7681 agcggcgacc aggcaaaaac tttcttaagg agttcccagc cagcctcaaa ataaggacca
     7741 atggtttccc agagtttctt aaaataaggt ccgacaacat cccagttagt gataattaat
     7801 cccgcagcca gggctatcgc cgtcgcaatc atgccaatcg gcgtcatcga catgatcctg
     7861 ctgacaatac tgatggcacc gccaacgccc atcaatccca gtttcagaat cgcaagaccg
     7921 gcagcaagcc cgacgacgcc gcgaataacc cggggatttt tatccgcaaa cttcgtgaat
     7981 ttctccccca actcccccag ccactgcgtg atatttttag cgtcaccaga aaatgcgccg
     8041 ccaatagccg caaggccgtt agttgcggtc cctgtcattg cctcccacag gttggacagc
     8101 gtaccaagct gtgcctgaac acgtttattc aggctggcct gtttattcat cttctgctgg
     8161 atctgatcgt agccatcctt tcctttatcg attagtgcat tgaccacctg aagggtttcg
     8221 gcatcatcac caaatattgc cttaagtaca cctgttcgct taacgtcggt cagttttcgc
     8281 agctttgcca gttgcctgaa catgttatca agaccgccaa aacttccttt gccgtcagta
     8341 aaatcgagct gtaccccgag tttctggcgg gccataactt tattaacgtc cctgattttc
     8401 ttaacgctta atccggactg gataactttt cgcagggcat tacctgccga ctccccgttc
     8461 atccccatct gatccatcat gacgctgatg ggggcaaggc tctgtgcagc ctgaagaccg
     8521 tccttgttca ccatcttcag aacagaactg gttttagtga agaaggacaa catgttggta
     8581 tcgtcaacgc ccagataaaa cgccttctgg atagtgtcga acagccccat catgtcttct
     8641 gacgccgttc cggtagcatc ctgcatcttt gcagcaaact cagcagccgc ttccggtgtt
     8701 tttttcagtt gtaccgcaag ataagctgtc gctttaccca caccacccag aatgttttct
     8761 gccgggatcc cctgacgcac cagcatctgc atcatgttct ggaaatcagc cgttgtaccg
     8821 ggtagctggt tacccaggcc aatagccagt ttattgatgt cctgaaagct ctttccaacc
     8881 tcgccgttcg catccatcat ggcgactttc agcccggtgg cggcgttttc ctgatcggca
     8941 taagatttca gggaaagcgt cagacccgct gccagtccgc caccaagcgc cagcccaccc
     9001 tgtgacgctt cttccgcctg gcgtttaaat ccccggattt tcttttgcat tttcgacagc
     9061 gcgggagaaa gcctgtcgac accggtgatc aacgccttaa gctcaaattc agccatgtgt
     9121 gcgtttctcc tgctctatcc tgtttgcctg actgaccagc aagggaattt cactgatcgg
     9181 catattcagc aattcgaagg gattaatgcg ccagtagctg gcgcagtcaa agaagcgatc
     9241 agtgaggtat tcagccgtca ggcctggagg aaaaaaccag ccacaagcca cgccgctgca
     9301 ttcaggtctg ccggagacat ctggtcgaca gagctttgcg gcactttcgc cagccgcaca
     9361 atgtatttcg acaccacatg cgccagaagt ctgacggact catcctgatt catctggtag
     9421 ggatacccca gctcgcggac atccttcccg gtgggttcat caaactccag tacggagagt
     9481 gtctcaccat gagcgataat cggtttcttt aactcaagct ctttcattac tggtaatccc
     9541 cttcttcacc gtggaactca agatcaaccg tgccttcttc ggcattatgg ttcgcttctc
     9601 cgtgcagcca ggcggacgac agtacataga cctgaccgtt cgccagctcg gcagtgatgg
     9661 tcatctcatc agacgaggtg attttgctca ccggaaaatt cttcggcacc ttgaaggtcc
     9721 ctttgacata aggcgcacgg tgagtttcct tgcggtccac tgaaccgtcc aggccgatga
     9781 tgtcatcatt gaccgtcctg ttcatgggca cctcaatgcc gccggtcagc gatagctgct
     9841 gaccgtcaat tttgaaataa caggttcccc cgatacgggc cattatgcag actcctctga
     9901 atactgaaga cggaactggt taaccacggc aaagacacgc aactggttaa catagtcagg
     9961 cgggaacagc gtgttcaggc ggttcggatc gctggcatca cgctccacaa ccaggtactg
    10021 cttaaacagt tcgtagtttt ccacgatccc cgcacgctca agctgacggt aggttgccag
    10081 cagttcccct ttgatcaccg ccggtgtgac aatcgcctga ccgggaccaa agcgggtacc
    10141 gtcgctggca agcttgtgac gcccgtactt actggtaatg acggatttca gtttgcgcag
    10201 tacatacgcg ctggtatgca gtgtctcact gtcgaggtag ctgttatccg caaccccgta
    10261 agcgtttttc ctgtacgtgg tgacatcacg ctgaatgcgc agtaccccgc tttcgacata
    10321 cgccgttgcc acgccatgag acagcagggt ctgttgttcg gtcatcgtga accgtttccc
    10381 cttcggcgca ggcagcatac ccaccagctc accggtctgc gtgggacgtg ccggatcgtt
    10441 gcggataaac accgctgcgc gggcggtacg gcttgccgcc agctcgtcgg caggcgtctg
    10501 ggtgtctttt tcgtaccccg ccagggtaat gtgctgctgg ttaaactggt cacctgcggt
    10561 caccagttct gacagcgtgc cgatctttgc cgaatacaca tgaccataca gctgacgcgc
    10621 atagctccag cgaccgctgg tatcgttcat ctcggtcacc agcgtgttaa cggaggccgt
    10681 gtcgttgaac ggcaggccga tataatcaaa cggctcatcc gccattgcag ccaccgcgcc
    10741 ggtgagaaca ggagcgcccg ttccggcggt acccgtcgcc acggcaatct gtacgcccgc
    10801 tggcagcact tcgcccccac caaagccgta gtaattgagg ctgacaggaa tttcattccc
    10861 gcaaagcccc ttatgacgcg cggtcagtgt gaccacgcct gccgaagatg aggcagtaaa
    10921 cggcagggtc ggaacggcat tgatggcatc ctggatactg ctggcaatcg tcgcaacgtt
    10981 atcgccgttg gtcaccggtg cctgcacgcg ggtacgtccc acataaacat tcaccgtgcc
    11041 ggtttcggtt gccgcgccgg tcaccgtcag cgtaaccgtt gccgccgcgc ccgtggattc
    11101 aggaacggca atcacataca gctcgccaaa cgggtcggtc tggcgataag cctcgaccat
    11161 acgcgccaac tgacttcccg caccacaaat ctggcgtgca tagtctgccg acggcatcag
    11221 caccagactg ttggcaacaa tctctgcacc gttattggca tgaccaatca gcagcgatgc
    11281 tccgctgtcc tgtgcagtat tcgccgcctg gttatccatt tccgcataaa acagcggaac
    11341 cagcgtattc gacggaatgg tgttaaagct tatcgtcatc ggtgttcacc tttttattca
    11401 cgcgccggat atcacccgct gcttcacggc gcagccagta gttgttctcg tcaacatttc
    11461 gcccttcggc gggcaaaagg tcaccgcggg cagggtcagg cactgaccgc cctttaacag
    11521 gtttcacaaa catgaagatt ctcaggaagg aagggttatt tcggtgtgat gttcgatatc
    11581 gccgtcaggc ccgttaccgg gatcgagata atcaacatca atcgccagcg ttcgcagttc
    11641 atccagactg ttcaggtcat cctgctggcg ggtatcgtct tcagtcagct cgctgatgat
    11701 cgaaaaatcg aactgataaa tcagctcatg acgattcaga tccagcagcg tgccgccgtc
    11761 atagttaatc gggttaccgc actcttccgg gttccagccc agcagggcct taaagagcat
    11821 ctgccggaca tcgtccacca catcatacga agcaaactga ccgcgctcat cacgcccgtt
    11881 actcagtatg acaaccacgg agaagccctc tttcagctcc tgccagtagt cggtctggct
    11941 tttgttttct cccggagaat cgtcccccgg taccacatac gccgccggga gtctcagctt
    12001 tccgacctcc ggcagatttt tgaactgtgc cgcgcctgcc acccggtttt caaaatacgg
    12061 gcagcgggca cgcagcgcag caataacagg cgtcagtttc atctgtgtcg tcgctccggc
    12121 ttcagtgatt tacgcaattc ccgcgccaga aaatagcgtg tccagctgcg gttcttttca
    12181 agagtttcca ccatgaagtt attacgtgga gccagtcgcc agccgctgcc accggatgca
    12241 ccacgatgat ggctgcgacg acgctttgcc cctcgcctca cgccatagaa caaaaaagcc
    12301 ggataaaaat caccggtgat acggcggttt ccctctccat tacgctggtt aggggctata
    12361 cgtgccataa aaccagggcg atgtttactg gctctgggta ccatgtaacc aatcgaacga
    12421 gccaggcgtc cggtctgata accggggttt tcacccggtg ccgaccgcgc acggcgcatc
    12481 accagccgac gggcatcacg catatgacgc tgaccaatcg tgacaaacgc ccgccggaca
    12541 cgggcgcggt taaagcgcat ctccgcgggc tgctgaaaat caacgtgcaa aaaggaagtc
    12601 gtcattgttg cctccgtgac tctgcctaca ttcgcccagc tccgtacact ccagcagcag
    12661 aaagcgccgc gccccgttca gatcgcgctg acgtttcacc cggtacacac tgtcaccgca
    12721 gaccacctca taatcagcgg tgatcccccg gcggtaacga atggtgatgt aatgggtgat
    12781 ggcgtccccg gtctgcgcgg tttcctgcca ggtggtggca ctggtctgga caaccttcgc
    12841 ccatgtccgg aacgcaaccg ggtattgagg ctccacgcca aagttatccg cgggcatatc
    12901 cacccgctgg cggatcagga cgcgtttatt cagttcaccg gggtccggca gaatgtaggt
    12961 tgcgctggtc tgcgcctgac gaattttcat tgcgggaaat acctgtacgg gccgacaagc
    13021 cagttaaagc tcattggcaa ctccattttc tcaacgtctg taaccgacga gcgattttcg
    13081 taaaaatggc tgataagcat cagcatcccc agacgaatat catccggcag gtgcagcccg
    13141 tccggatcgc tgtccggaat ggtttcatcc ggagcataga gcttccggtt cagatacgtt
    13201 tccgtccgct tttgcgccgc acaggccagc agttgcagat ggcggtcatc agcatcgaaa
    13261 tcctcatcca gccggagttg ggctttaatc tcttccattg tcagaagcat actcagccct
    13321 ctttactggt cgtggctttt ttctcttttg tcgctttact gctttttgca ctggttccgc
    13381 gctctgctaa cccggcctga agtgcaatct cctgcacccg ggcaggaagc gccccgtcgt
    13441 catactcacc ggcccgaatg acctcaacac gcataccgtc cggtgaccat ttcagatctt
    13501 gtttcaggat catgattctt cacccgtcag aacagggggc gcggttccgc gcccctgagt
    13561 gattacgccg cagcaatctt cagcagtttg atggcctgcg aatcgaccag catgccgccg
    13621 gtgcgcttgg tggtataaaa accgacaaac ggtttattgg tgtacggatc gcgaagaatg
    13681 cgggtaccga tacggtcaac gatggtgtaa ccccgtttga agttaccaaa tgcaatggct
    13741 ttcgcatccg cggcaatatc cggcatctgc tcgttttcag cgataccgta acccgccaga
    13801 gaggatggct gccccagttc cagcccagga cgccacagat agttaccctc ggtgtctttc
    13861 agcagacgga tggcaaacag gctgttgttg ttcatcatga acttcgcgcc agtgcggtgt
    13921 gccttacgca gcgtgtaaat cagtttgata atggcgtctg cggtcaccgc ggtcgcttcg
    13981 ccggatacaa tatgctgaag tttgccgaac gcccggacct tgtcggtttc atcagtggat
    14041 tcatacgcca ggaacccttt cggcttcttg gtgccatcgc ctgaggtaaa ggcaatttct
    14101 tcctgttcgg caaattcggt tgccagctcg ctgttgatcc aggcctccac gttgaagaag
    14161 gcatcgtcca gcattttctg ggtagcctgc gggttgccgt aaatttcccc catgagaggt
    14221 tcaatcagct ccagtctgga ggtggcagtc tgggatcgcg tatccgtttc ccccacccat
    14281 ccggaagccg taccgcccag attcaccagt tttttgtagt cggaaccgcc aacggtgatc
    14341 accgtggctt cctgacgcat caccacttca tctttcagca ggttaagaat gttgcgatcc
    14401 agttcttccg gcacggcgta gccaccgtct tcatcggtac ccacctgcaa tgccttacgc
    14461 tccagatcgc gcagaccgtc ttcacggcct ttacgcagga agcccacaaa cgcctcttta
    14521 tgctcggtgg ccagtttatt ttgcgcacca cctgccggac gtttcagctc aagcagctct
    14581 ttttcaagat cgcttttgag attttccagc tcgctgagtt tcccgttcag ggtttccacc
    14641 tgcccggcaa gcttgccttt ttcctgctca atcgcatcca cgcgcttgtc gttctttgct
    14701 ttgaagtcgt caaacttctg ctgcagctcc tgcgcgacct gttcgacatc tttaatatca
    14761 accgccatcg tatttctcct gattagaagt tcagattttt cagtgcattc agtgcagagc
    14821 ccacatcctc agcgtcgcgc agggacagtg cgccatagcc cccggccatg aatgctttgg
    14881 cctgggtacg ggagagtccg acatcacgca ggactctttc gatttttttc tgttcgggga
    14941 tttccccgcg ggccagtgcg ttcttgacgt cgctgatccg cgcctcgtcg ttagacggga
    15001 acgtcaccag gctgacttcc cagaggtcga tttctttcag cagaaaggct tctttgctcc
    15061 ggtcgtattc ccagtctttc aggacgtacc caatagaaag gccggttaac gaaccggcct
    15121 tcatgtgtgc atgtgcgcgt tttgcgaggg gatcatcatc aataagcaac cgtcccctga
    15181 cgtaaagccc gacatcgtct tccttcattt cggtgtaaac accgatgggt tcatccatgc
    15241 ggtgctgcca gagcagcgca ggtaacgctt ttctgtcact ccacgcccgc agggaagcag
    15301 caaatgcccc ggacatcacc acatcatcgt ggctgtcctt tacaccaaag acggagccat
    15361 acccttcaaa ctcaccggag tcactgacag atttcagact cagcggtaca tcaagacgtt
    15421 gtttcgtctg cattggcgtt atccttctgc ttaccggctt tactgccatc ggagggtttc
    15481 gtggtcatgt tcatcggtgt gagatagaca ttcccaccgg gacgcggatt catatcttcc
    15541 aggtcgcggc agtcattggg agagtaaatt ccccagttaa tcccggtggc gtaggcttca
    15601 aaacgggact tcatatcccc gcgcagtaac gccccggcgt taaatttggc gtaataaacg
    15661 ccctgcttac tttttcgtac cagtccggtg ttgatccgct gttcgatgcg ggtcagatac
    15721 ggcaccagtg aatagttgat aaatccgagc cccagttctt cgatattgtt gaaggtggcg
    15781 cgatcggtgt tctgcaccat gtgcaatggc acccggaaca gacgacagat ttcttcaagc
    15841 tgaaacttgc gggtttccag gaactggctg tcctctgcgt tcaacgccat cgacttccag
    15901 tccagcccca tctcaaggat catcgggcgg tgagcattgc caagcccggt gtgacgctcc
    15961 tcaaaatctt tcttcaggcg ctcataagcc tgatccgaca gcgtctgctc tgtacgcaac
    16021 acacccgacg tcaccgcgcc attgctgaac agtctggccc cgtgctcttc ggtcgctgcc
    16081 gccagcgata ttgcctcgcg ggcataggcg atgggattca gtcccaccag accgtccagc
    16141 gtcagcgtgc gcacatgcca gatatcttcc tggctcagta catccgtgga gccgtccgga
    16201 aatgtgacct ggtaaaccgg ttcccagcta ctgttaagct tcggtaccac acaaccggga
    16261 tcgacgggca gcagttcagc cacttcgcca aatgctttca ctttgtaggc gtaaaaattt
    16321 ccccgcaggc acagacaggt gaccaccagc tcccagaact cctgcggcgt catatagcta
    16381 ttgggatgcg tggagatcag tttatgcaga cgttcgccgg tggctctctg cttcaggctg
    16441 ccgttcaggt gatacagatt gcagggcaac atcccgaccg actctgccag cactctgacg
    16501 caggaaaaaa ccgccgtcag tcgcatggct cgctgactgc tgatctgctt tccggtatag
    16561 gtgtcgtagg acaacccgat ggcatccgcc agctctgctg acgtggtcac cggtgcgtca
    16621 ctttttcgtt gaaataatcc cgaaaagaac actatttacc tccaccaaca gacatctgtg
    16681 tacggtcgag atatcgcgcc accagccacg accagaacag gcacaacgcc ccggcaacaa
    16741 caaaccccgc cgggggataa atcagccagg caccatacgc cagcaaaagc gcccccagca
    16801 cgcccaccag aggcgcgaga atcagcatga tcataattac ctcagttaaa gcgagcggat
    16861 cccataggac tcaatgtggt cagacagcgt gtcttctttc tcgtacagca tggctctgcc
    16921 aaccgccata atcagcgcaa ctgcaccgtc aattttgttt tccgcctgct ctttgacggg
    16981 tttcaccaca tcatcgttac ccggcatgtt tttgccgacc acgttgccga tacaccaggt
    17041 catgatggga ttgccgtcat gatgaaaacg tcccgattca atcgctgctt ccagctcttt
    17101 catcggatcg gacatattgg tgaagttctg gaagatagtg acgggattca ggtcttcatc
    17161 agcaaggtca tgtgacagcc cggtcgcccc gaaggggtcg atgggtgact cgctgaccgg
    17221 gctgattttg ttcgccgctt tggcctcttc gaggatgtag cgataatcca cctctgcacc
    17281 atcggtaacg gtcaggatgc ccatttccac ccatttctga aagcgttcgg ctgtccggcg
    17341 atcttcattt ttctcgacgc tgtacaccgt gtcatacggt acccagaaac gcggggccac
    17401 actgtagtaa tgcgttttac cgtcaatctc gcgggtataa agtcgcgcca tgctgttcat
    17461 atccagttta cgcgccaggt caaaggccag aatgcacggc tgcccctcga attgctcaag
    17521 agtcagtgat ttatcctcgc agctctgcca gctcaccagg ttgaaatacg ccgaacgcgc
    17581 cgacacccag atattgaggt gttttgtttt aaagacgttt gccagacggg cgttattttt
    17641 cgcacgctgt tgctgactta acaaaaactc acggtaaacc gacaccccga tattcgggtt
    17701 ggctttttcc agcacctgtg ggttggtcca gtcgtcacct tcgtcaacgg tatagatgat
    17761 cccgaacagt tcatcgttgg gtaccgaacc gttgagcatc tcgataactt cccgccgttt
    17821 gtcgtagcac ggcccctcaa tgttgtaccc ggcagtagta atggcccaca tcagtggctg
    17881 acgtcgcgcc cccatcccgg taagcatcgt ggtgtaaagc gcatctgtgg cgtgctcgtg
    17941 atattcatcc accaccgcac agtggggtga tgaaccatca ccggggttac cgatcagcgg
    18001 ttcaaaacgc gcaccatcct ccggacggtt catgtttgag gcgttaacct caatcccgaa
    18061 cgcttccatc agcatgggtg tgcgtttaca catcagtctt gccggacgaa agacttccca
    18121 tgcctgtttc tccgtcgtgg caccggaata cacttccgcg ccgaactcgt tatcacaggc
    18181 aaaacaatac agggcgacac cggcagagat tgccgatttg ccgttcttac gggggatttc
    18241 ggtatacacc tcacggaagc ggcgcagccg ggtaccttta ttgacccagc caaacgcgca
    18301 gcagatcaca aagagctgcc acggctccag cgtgatgggc atcctcttaa atgcccactc
    18361 acccttggtg tgcggcaaca gctgaataaa tttggcggcc cgttcagcca ggtccttgtc
    18421 gaagcggtaa cgaaacgact tatttttttc cgccatcagg tcatcaagat ggcgctggca
    18481 ggcctgaatc acaaactggc aggcaacaat ctttccgcgc acgacatccc gggcatactg
    18541 atttgcagca tttacgttgg ggtaagattt ccggctcatg attcgatgat tttcagaaac
    18601 gggttagtgg ctttcttctg ccccgccagg ccaatcagac gctggcggct gctggggtcg
    18661 agtccgagca ttgcccccgt gctgctcatc tcggactcct gttctttctt ggcggtcagc
    18721 tccggatttt taaccatgcc acccattgca ccggtgatgg tgttgccctg tctggcaata
    18781 tttttcacgg cacgtcgcca gaactcatag gccacgcacc accgctcaag taccgcaagg
    18841 tcagtcacgc acagcaggcc ctgaccgcag agttctttgg ttgtcagttg ccacatgatc
    18901 gtggcgagag ggagatcttc ttctgcgaac cactccggtg gctcaacacc tttgatgggc
    18961 gtaaaaacag gttcatcttt gttcagggct cgcttgccgg ggtttccggc cagcgccttg
    19021 cgcgccgttg gcttggggcg acgcccggaa cgccccgccg ttccagccat atgcggcact
    19081 cctggttaaa tttcattttt cgcgggtata aaaaaacgac ggggcgggca gtccggaaga
    19141 cgtcaggtca cagggatttg acccgccctc ccctctggct gtgggaactg gttcttactt
    19201 caaccgttca cgggccgtct tcgccttatg acatggccag cacaggctct gcagattact
    19261 gtcagcgtca gtgccgccat gcgccttagg gataatgtgg tcaacggttt tcgcctcacg
    19321 caccacaccg gcacgcagac ataactgaca caggcctttg tcacgcttca gcacacgttc
    19381 acggatggta tcccacttcg aaccgtaacc gcgctgatga cgtgacaggc ctggtttgta
    19441 ttgcttccag ccttcgcttt tgtggctttc gcaatagcct gacgggtcag tagtggtatt
    19501 gcggcaaccg cgaacgcggc aggcttttgg tgtacgtggt ggcattaatt gtttccttaa
    19561 gccattacga tgcgtctgaa aatgagttaa tctgaaaatt ccttcactat gcgagagcat
    19621 gacaatggtc ataggacaag aagatcagaa atgctgcccg gcttgcaatt cagatgcgac
    19681 ctggcagaac cgtgataccg cttggttaat tagatgcccg atgtgtgaaa catttctcat
    19741 caggaattcc accattgaaa ttttgcgaag tgacgttgtg tatcggacat tagctggtga
    19801 ccttctcaaa caagaaggtg ggtgtgacta catgcttaca agaggaaggc ttgcgaactt
    19861 cgcgaaaacc cagctcccaa aatcaaagtt tcaggaatac ttccctggtg acaactacga
    19921 ataagccatt acaaagccca cccgcagatg agctttgtaa tggctactgt gagttgcgtg
    19981 acgtttctat cttcctgata ctggccttgt cgatgttgca ctggcccagc gccgaaagca
    20041 ggctcacatt cagatccaga ctggccccat aggtcagcgg gtcgggaatg actggctggg
    20101 gcgtttcagt ggtcaggctt gttggcagcg gtaccgccgg aaccggcacg taaactgttc
    20161 gcgtacttcc gcaaccggtc agcagcggca gcaggcacat gacgtgaagc acaatcatca
    20221 tccgcaacag ccactttgat atcttcctgg gttctctgtg actccagtgc gatctgctgt
    20281 tttgcatgct ggttagcctc tataactgta ttgatgattt gcagtgattg cagaacgtta
    20341 cgggtaatgg cagttgctga ttcagcattt cgtacagcct catcagcacg ctccttttcg
    20401 tgctgatatt tgctgtagta atgtccagca gaccagatga aggaaccaat gacgctaaca
    20461 acgaaggcaa caataaccag cttatatctc agcttcattt actaccccac cagctttttt
    20521 aaatcgggca atcaggtcac cgattttatg ttcatactga ccgtaacctg caccaggtaa
    20581 cgacgcccag atattgctgc aacggtcgat agcctgacag atatcaccgc gatcaatcat
    20641 cggtaaagcg ccacgctctt taatctgctg caatgccaca gcgtcttggc ttttcggaga
    20701 gaagtctttc aggccaagct gcttacgata ggaatcccac caacgggaaa gaagctggta
    20761 gcgcccggct gctgttgatt tgagttttgg gtttagcgtg acaagtttgc gagggtgatc
    20821 ggagtaatca gtaaatagct ctccgcctac aatgacgtca taaccatgat ttctggtttt
    20881 ctgccgtccg ttatccgttc cttctgacca tgccaccata tcgaggaaag ccttacgctg
    20941 aggattaaga ttttgcattt ttcacccctg tcagtcgttc ccagaagtac gtcagtgcaa
    21001 ccgaacccat cgcaccacta atccccgctg tcgcgagaat catgtaaata ctgaatccac
    21061 tttcgatgct gatcaggcca ccaataacac cggtgaatcc tgataccact atctgagcca
    21121 gagcatttat ccaactccac gttgctttac tctgcttcac atctatcagg tagcggacca
    21181 gaccgcccca acctgcgatg atcagcaaaa cgagccagaa cgctccggca aggctctctt
    21241 tttcgtgcat atgaatagcc aatgtttcgc cgccgacaaa aggccgggac gttaaatgtc
    21301 agaaatcagg ctcacggggt aatttaacga caaagcacgg agttgatgct ccccgcaagc
    21361 ctggaataaa aaagccagca tgtagctggc aacagagggt taagcaatat caactcaaca
    21421 gctgaagaca ccctggctgg ggtaggttgg aaggctactc accgtccaga aacagaaaag
    21481 cccaaagttt taaacctcgg gcttgaattt ggattactgc cagtgcgtac aacattggca
    21541 aaatatcaga tttacacgaa atatatgctt tttaatccag ttttgcaata ctttgctgtg
    21601 aaaatgtcgt cttttgtttt gaacgtgttt tcgttaaaag caataaagct tggctatcaa
    21661 gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt aaatgtctca gaccagtttt
    21721 ttgatgtcac tcccaccagt gatgccagct cctggtattc ataggtctta cgccctgcca
    21781 gctcgttctt cacatcctgt gccgccagcc agatcaactt ctttaagcgt tccagtgtct
    21841 tactggcaat ttttcttgta cctaacagag ccttaaactc gttccatgcc cactgcgtta
    21901 tggcaacctg atgctcccag cgaacacttt cgctgtaact ccacagcaac cacgctttct
    21961 ggtgttcttc gagagacaga accgcgcggc gccatgaaga tgttgagaac tcaacctggc
    22021 tgaccagtgc aatggatgaa ccttttgcgt acgactgctt accggaaatc ggcggattat
    22081 ccagcgtaat catcctgcca gttacctcat ccagaatgcg cggcttcttt cgtttgtatg
    22141 taccagtatc aaattgagca tgctccagcc aggcttcaag ctggcctttc gttactccgc
    22201 tcaaatcagc ggtagccacc atgagttgct cgcgaacata ctgtaaatat tggatattca
    22261 tgcggcagct cctttcagtg ttttggcgta attcttcagt attcggtaat cggtcaaaac
    22321 agaaccggga aaacgatata agcgcaggcg cacccagcgg cggcgaagac gttctgccat
    22381 ataagactca aacatcattc atctcccagt tcggtgatgg tcagctccaa cttcccacct
    22441 ttggtaacgg gcatcttcac aacgcgataa tcaacgacct gagcatcatc caaccagaaa
    22501 cctgctttgg tgagtgcgtc aaaagcggcc ttttgcagat tatccaggtc acggcgacgg
    22561 cgatccggca tgtggcactc aatgcggatt tttacaggca tagccaggcc gatatccagc
    22621 attgcgtttt taatgattct ggcgacgtta tcgcggtatg cctgcccctc tgcgctgacg
    22681 tgcgtgcgcc cgcgattatg gcggtaatag cgattattgc tcggaggcca gggtaatgtg
    22741 atgctgtagg tattcacgcc ttaataaccc cctctttcag ccagataacc tgtgttctcg
    22801 ccataccttc cagcgcgcat tcttttgcat atgcagcatc gacaaaatgt gtgcggcggt
    22861 cgatttcgtc gtggcaggca gaacatgcaa tggtggcaat caggtctggc ggtttggtac
    22921 cggtgccgca caatccagtc agccggatat gtgccagtac agacgtttca gggttgccat
    22981 tacatacgcc agggattctt acctggcatt cccgaccacg cgctgctttt ctcaaatcag
    23041 ccatgattcc tccttgctgc cagtcgcaac cattttttat caaccaggct ggcggtatat
    23101 ccgagcagtg ttggtatttc ggaaggcttc agctcaggtt tacgcttacg acgatttggt
    23161 actctgtaaa tgtgcccgtt catgacacga ataagcggtg tagccattac gcctcctgct
    23221 tatcgcgcag cagctggaac tcgcagctcg gcggaatagt caggtggcag ccaatattca
    23281 tcgcccaggc ttcaacctta cacaggaaga catacatctc tccggtatca agatcggagg
    23341 tatggcgtaa cgactggata gtggtgatat caccggttac gacatcaacc aggtctttgg
    23401 tttcataacc gagatatgtg tgtttgagag catcttttac ccaagctgga gtggcgaacg
    23461 ttttacccct gctgatgagg tattcactga tttcgctgta ccacatgtgg ctgagtgcat
    23521 tctgggaaag actgcgtctc tcgcgccacg gtttaagcac catgcgaaag catttgcctt
    23581 cctccagata aggctggatc tgctggccga tagcggtgaa gttgccgcga tgtaatttga
    23641 tgccgtcttg tgggaggttc acgcttcacc tccgcagagg tcaaacgctg gatgcaaaaa
    23701 atcgcaggtg catttctgca tctgtgacag caaaagagag tttagattgt atgtgcgcat
    23761 aaacgtcccc gtttagcgca gaagtcaccg atgttgttca ggctccggtg acattattat
    23821 tgctggttga ttatggaaaa tcaaaggtag attaatttaa taatccttgg agaaatctca
    23881 cgcaccacct caatagttgc gcttgctctt gagaatcaac aaaaagataa gcgtctttat
    23941 ttccgtggaa aaggttattt cttatcctta taactatctt taatactgta gacatggtgt
    24001 ttagatctgt tgctgcgtca ttttgaagtc cattaactat tccccgccta tctctctctc
    24061 gaccgacaca gagggcgtca agtctttcct gcgcatcccc gttcaaaatg tatctctgct
    24121 taaaatcgtg aaaaagttcg ttgggcacct ttatcccggc gtcaagtaac gttcgcgcat
    24181 aatttaaact gtcttccccc ttagcaaagc caccaaaaaa actttgctcg accctaggaa
    24241 aaatcatgca gaattgaaga taaacatcaa tcagttcatc agatggaggc tccgataaat
    24301 taagtaaccg ctgcagtcct tctgcactta tcatttttca ccttcctgct taagatgtcg
    24361 aggagcggat gccttgggca gcgcgatgct ctgctcgcgg taatgtcgtg gccgctccaa
    24421 aaaatagtcc cgcaaatgct caggctgctc acgcatcacc gcttcggcaa taaccggtat
    24481 attaaggcgc tctttgtagg taactcagga gacagcaagg tcaacgttaa ccttctcgcg
    24541 ctcttcttgt gttttagcgg caatattccg cttcgacatg gcgttacgtt ttactccttt
    24601 ttgggtgcat acacgttgtt tatccgttca atatgaatag ttacatccgg gctttctaag
    24661 aataagaagt caatcaaatg accaatgaga atacaggcat cattaataga ggaccttgtg
    24721 actttaatcc ctccatgact gccgtgattc cccaaccact ttaacgccat gaatgcatct
    24781 ttatttgatc ccagtttatc tgaataattt gtgattcttt catgcgtaga aagacgtatt
    24841 atcttgcctt taactgtttt ctctcttggt acatcaagac tatcaagcat catctccagt
    24901 atggttctca tggtgttcac cgctgaagct gggtgcgagg ggatcagagc tgatacttcc
    24961 gccaagagat cagctatatc ttcagggcac ttttcaggaa acaggaatag aggaagtggt
    25021 ggatagaaat acttaggacg gaaaccagaa acataccaac gctccgtcat ttcgtcattg
    25081 aagcattcta cagcaacaac ctgcccagac aaagcaacat tctcctgaca ggcttggcgg
    25141 gtacagcgca gcacgcaact gaatactcct cccgacattt cctcatcaaa ccatacttca
    25201 tctctaaact gagttgttgc gcttgaatca gtttctacaa aactatcagc gacaatctcc
    25261 agtgtttcat tcatacagtt cgggcagcgc caagctggtg cgtcatttcg tttaaacgtg
    25321 gcatacagta atttaggatc catgtttttt tctccgtgct taacgttttt ggttgcgtta
    25381 acactctata ggattttgca gcctttacta gttcataccc aaccggtctt agtaggcata
    25441 ttgaattaca atcagctaat taagttcctc tacatcgagg gtatagtcgc atcagcaaca
    25501 cggaactcgc gctcaaattc tgcaagatcc tgctcaagtg caacgtagtc ttcgcaacac
    25561 aaccatgacg ccctcagcac tctctaccac atcgcaatgg cacgaatacc cacacactca
    25621 taacgtttcg cttatacccc tacccccccc caaaaacatc aatacccgtt tcatcacggc
    25681 actctggcga cactccttga aaatcaggtt cgtgctcacc tttccttccc gctcctccct
    25741 ggtagcgaac cggtaataca cctttcgcca gaccttgcca tcaacgacca ggattcctgc
    25801 ccgtgccatt ttggctgctg cctgattgat gctggttaca gtcacgcctg ttgccgcagc
    25861 tacatccggc gcgcagaaag tccggtgcgt tttcaggtag tgaagaattg cctctttgcc
    25921 tgtcatacac tggctccttt cagtccgaac ttagctttga tttctgcgat cttcgccaga
    25981 gcctgagccc ggttaagtgg tctaccgccc atgacaggaa gttgttttac tggttcaggt
    26041 atagcctcac cacggttaat tcgtgcggtc atacaggaca gttcatcggc agccttgcgc
    26101 cgtaattccg cgtcagtcaa cgcattggcc cgcatgttct gatacaggtt ggtaaccagc
    26161 cagtagtgcg cgtttgattt ccacggataa gactctgcat ccggatacag cccgcgcttc
    26221 cggcaatact cgtaaaccat atcaaccagc tcgctggcgt ttggcagccc ggcggtaacg
    26281 gatgcttctt cccggcacca ggcgacaaac tgcccgggtg atggcaggaa tggtcgattc
    26341 tgccgacggg ctacgcgcat tccagcgtta acctgttcca ttgtggtgat cccgttttcc
    26401 cggaaagcca gaacccactg gcggcgaatt tcgttcagtt cgttctggtc acggttagcc
    26461 aggctcgcag ggaaagttgc cagcaactgg ctgaatacac cgttgatgat ctgcgctacc
    26521 tgctgtacct gcggcttttc gtcgtactgt tccggcatgt tgttggcgat ccgacgcatc
    26581 tgctcacggt caaagttaat catctgtgcg gcgatgtttt tcatagatcc accccgtaaa
    26641 tccagtctgt gtttgtcagg tcgagttttg attttccggc tgtcacgcca gcctgttgtt
    26701 tgttacggtt gatttcgagt tgggtccact tgtcgcgaag tttggccggg ctcagcacgt
    26761 taccagacca aaagttgtcc tggcatgccc agcggaacag cacgcacatg tcgcggtggt
    26821 tacgtccgtc acgttcacgc atcaggcgga tatcgttagc ccatcctgca aaattcggtt
    26881 ttctggctga tggcgcgatg gtcttcacca tgtcaaacat ccactctgcg gcggtcaggt
    26941 cttctgctgt cccccacttg ctgccgctct gaattgcagc atctggtttc accacaggaa
    27001 gatcgttttc tggttggtca gaggattcgc cagaattctc ggacgaaaaa ggttttatat
    27061 tgtcttttgt tagtttgtct tttgtgttta cctgattcgg gtaaacgcct ttacctgatt
    27121 tgggtaaact tttcttacct gattcaggta aatttacctc tttcaggtaa actttatttt
    27181 tcttacctga ttcgggtaat gttgaccatt cactgaccac attattaatg ccgatattcc
    27241 gcccgctctg aataaaaatc ccacgcttta ccagaacact ttttgcagca gaacacttgt
    27301 gcggcaatat cccggttaat tcggaaagtt gctcgttgct aacccaatcc tgttttttat
    27361 taaagccata tgttttgcgc atgacagcca gaaagaccag aagctggtgc tgtgttaatc
    27421 cggccagcat cacagcttcc agcaactcat ttgcaatgcg cgtataacca tcatcgagat
    27481 ctgccacgcg ccgctccttt tgtgcctcat ccggcactgg aaaattgaat atctcagcag
    27541 tgtttgccat aattcctccc gcaatgagtg tgttacgatt tgcacctgaa agtcggttct
    27601 gttcgcgcag accggctttc gccatttccg aacctgtcat attgccccca gcatggtggt
    27661 gaccatcgcc atcagtggac cagccagatc cgggtccaca cgaaacatcg acacaatgcc
    27721 ttcactcatc tccttcagtt tctggtggcg tggtgcgttg agaatgacag cctgttttgc
    27781 ctcactgagt tccttttcca tttcagccag ccgagccatg tagctatcct gctcaaccag
    27841 gtggccgcga tattccagcg gtagtaccgc cagaattgcc ggggtcagtt cacgcacgtt
    27901 atttcggtat ttttcagaat cgaatttgtt atcgaggaag cggaacagct tctggcgtgc
    27961 acgactgaca tcatcaggga aatcgatggt gccgccgccc tgctcccgat actcattcac
    28021 aatgagtgcg gcaacaacat cctgattatc tgcagccgac caggcgcgaa cggcatcacg
    28081 gattttttcg tggcctggcg cctgttttgt ttgagaacga tttatcaccg cagtcgggct
    28141 aaatccgcta gtctgttggt atgtaagtgg ttgcatagtc attgccttat cagttaacgc
    28201 cgcagtttag gcggcagaat tactcgcgtt aaacaatggt gcgaggtcgg gacgaatatc
    28261 tgctggttta atctttccac cagtggctga gacaattttc attacatagc gggcatcaat
    28321 tccgccaccg tgtagccaac gccaaactgt gggttgggct acaccgcata gatctgccag
    28381 tcgtttttga ctacctgtaa tactgattgc gagttgaatg gtttgatttg tcattatcaa
    28441 ttcctattgg tattgcaatg aatgaataat agcaatgcgt attaacccaa gcaatagcaa
    28501 aacgtgtttt gaccatcaat acgcaagcgt ataaattaaa acttatgaaa aaagaaactc
    28561 ttgctgatcg cttaaaccta gcgatggaac aatctggaat gtctcaaggc gctcttgcaa
    28621 aggcgtctgg cgtagctcaa cccacaatct ggagactgac aagcggcaac gcgcgcggct
    28681 caacaaaaat tgttgaaata gctaatgcat tgggtgttcg aacagaatgg ctctcatcag
    28741 gcataggccc gatgagaaat gacggtcaac aatcagggaa gcctgctgtc agccattcca
    28801 aatacttcaa aattgacgtt cttgatatag aagtcagtgc cgggccgggt gtaatcaacc
    28861 gtgagtttgt agaagttcta cgctcggttg agtactcgtt tgacgatgct cgtcacatgt
    28921 tcgatggcag gaaggcggaa aatatccgca tcattaacgt gcgtggtgac agcatgtcag
    28981 gaacgatcga accaggtgat ctgctgttcg ttgatatcac ggttaaatct ttcgacggtg
    29041 atggtatcta tgcgtttctg tacgacgaca cagcccatgt aaagcgtctg caaatgatga
    29101 aggataagct gctggttatc tctgataaca agagctactc accgtgggac ccgatcgaga
    29161 aagacgagat gaaccgggtg ttcatcttcg gtaaggttat tgggagcatg ccgcagacgt
    29221 ataggaagca tgggtagcgg attagctaat cattatgatg ttgttcaaat ttaagaggtc
    29281 cgcatgtctt cgatttgggg aaaaaagttg atgacgaaag aacaagagcc aaacactgat
    29341 gaagtgaaac cagagcaagg aagtttgttt gaattagaat ttcatgaata tacaccttca
    29401 agcttaggca ctgcacacct ttctggtttc gctattgcca ctgatgaaat ggaatatgca
    29461 gttaaaagca tgcgttccac cccacctttt cctgtacaaa acccaactca agtaccggca
    29521 gcggaatggt tttgcacaaa tttagctgaa aaatgcggca tcgctacacc tgtgtgcaaa
    29581 attctcaagt gtattgctaa tggggagtat gtttttggct ctcgcataga gttttcagcc
    29641 tggaaatctg gtctcaatag tcctcaatgg ataaatttgc tctcaaatgc ttctgaatcc
    29701 ttaaagaagc aactctgggc aatttatgca tttgatcaat ttgtatataa cattgacaga
    29761 cacctcaaca actacttata catggaaaac acccgaggca atgttatagt taaaacattc
    29821 gattttagtc taagctcttt tgttataggc tggccacgaa atacccctca tatactacca
    29881 acagactcca gtacaacaac aaactggacg attgctaaac aattcattgg cgacactgat
    29941 gatctgagaa agtgcgcatt atctgtcctt gacaaaattg aaaaaattgg agttgagact
    30001 atatctgata tacttaactc tatgcctgaa gcatggatgc caccaatgca taaggagtat
    30061 tttttgaagt ggtgggacag cgaggagcga atcgataggc taaatgccat aagactggag
    30121 ataacatctt gaaaactttc aaatacagct taatcagagt tacaccgaac ttagagaaag
    30181 gtgaaacgat taacgttggt ttgatcgttt accatgactc tgacatagac gtacgtatgc
    30241 tcaattcagt ttcaaaactt aaagcgattg acaaagatct taccttagat tacttagagg
    30301 atctttcaac ctcgcttttt gatttgtcac aaaaaatcaa caatgctgaa cttcttcctt
    30361 gtctttttaa gggagcatta tcgttatcgt catttggcat gttcactctt caagccaacg
    30421 aagattacga agctaaagtt actgatctaa tgaatcggtt agtaaaccca caaaaacaac
    30481 cacataagca gttgaaaaaa aaggtttttt ctgaattaaa atccaccttt a
//
LOCUS       DAYPHN010000042        30080 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000042 DAYPHN010000000
VERSION     DAYPHN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.042"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            18..203
                     /locus_tag="ACG0BW_001869"
     CDS             18..203
                     /locus_tag="ACG0BW_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258838.1"
                     /translation="MSRKYLSAMITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            164..283
                     /locus_tag="ACG0BW_001870"
     CDS             164..283
                     /locus_tag="ACG0BW_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFK7258839.1"
                     /translation="MPLSYAGYHHFIMMSLMSGEHNTLALSLQDQSPGLVPAG"
     gene            318..662
                     /locus_tag="ACG0BW_001871"
                     /pseudo
     CDS             318..662
                     /locus_tag="ACG0BW_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1027..1293)
                     /gene="minE"
                     /locus_tag="ACG0BW_001872"
     CDS             complement(1027..1293)
                     /gene="minE"
                     /locus_tag="ACG0BW_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFK7258840.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1297..2109)
                     /gene="minD"
                     /locus_tag="ACG0BW_001873"
     CDS             complement(1297..2109)
                     /gene="minD"
                     /locus_tag="ACG0BW_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFK7258841.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2133..2828)
                     /gene="minC"
                     /locus_tag="ACG0BW_001874"
     CDS             complement(2133..2828)
                     /gene="minC"
                     /locus_tag="ACG0BW_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFK7258842.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3348..3716
                     /gene="ycgJ"
                     /locus_tag="ACG0BW_001875"
     CDS             3348..3716
                     /gene="ycgJ"
                     /locus_tag="ACG0BW_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFK7258843.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3819..4220)
                     /gene="pliG"
                     /locus_tag="ACG0BW_001876"
     CDS             complement(3819..4220)
                     /gene="pliG"
                     /locus_tag="ACG0BW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFK7258844.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4291..4449
                     /locus_tag="ACG0BW_001877"
     CDS             4291..4449
                     /locus_tag="ACG0BW_001877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258845.1"
                     /translation="MAFLRSVKSRRGIFAGGSLIKGAWTEQTAEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4461..4754
                     /locus_tag="ACG0BW_001878"
     CDS             4461..4754
                     /locus_tag="ACG0BW_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFK7258846.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTHDTNK"
     gene            4826..5485
                     /gene="ycgM"
                     /locus_tag="ACG0BW_001879"
     CDS             4826..5485
                     /gene="ycgM"
                     /locus_tag="ACG0BW_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFK7258847.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5577..6023
                     /gene="ycgN"
                     /locus_tag="ACG0BW_001880"
     CDS             5577..6023
                     /gene="ycgN"
                     /locus_tag="ACG0BW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFK7258848.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6230..7141)
                     /gene="hlyE"
                     /locus_tag="ACG0BW_001881"
     CDS             complement(6230..7141)
                     /gene="hlyE"
                     /locus_tag="ACG0BW_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFK7258849.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTAEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            7513..7932
                     /gene="umuD"
                     /locus_tag="ACG0BW_001882"
     CDS             7513..7932
                     /gene="umuD"
                     /locus_tag="ACG0BW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFK7258850.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7932..9200
                     /gene="umuC"
                     /locus_tag="ACG0BW_001883"
     CDS             7932..9200
                     /gene="umuC"
                     /locus_tag="ACG0BW_001883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFK7258851.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDLGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(9246..9776)
                     /gene="dsbB"
                     /locus_tag="ACG0BW_001884"
     CDS             complement(9246..9776)
                     /gene="dsbB"
                     /locus_tag="ACG0BW_001884"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFK7258852.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9922..11463)
                     /gene="nhaB"
                     /locus_tag="ACG0BW_001885"
     CDS             complement(9922..11463)
                     /gene="nhaB"
                     /locus_tag="ACG0BW_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFK7258853.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            11685..12404
                     /gene="fadR"
                     /locus_tag="ACG0BW_001886"
     CDS             11685..12404
                     /gene="fadR"
                     /locus_tag="ACG0BW_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFK7258854.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(12455..13987)
                     /gene="ycgB"
                     /locus_tag="ACG0BW_001887"
     CDS             complement(12455..13987)
                     /gene="ycgB"
                     /locus_tag="ACG0BW_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFK7258855.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            14317..15615
                     /gene="dadA"
                     /locus_tag="ACG0BW_001888"
     CDS             14317..15615
                     /gene="dadA"
                     /locus_tag="ACG0BW_001888"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFK7258856.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            15625..16695
                     /gene="dadX"
                     /locus_tag="ACG0BW_001889"
     CDS             15625..16695
                     /gene="dadX"
                     /locus_tag="ACG0BW_001889"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFK7258857.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16863..18299)
                     /locus_tag="ACG0BW_001890"
     CDS             complement(16863..18299)
                     /locus_tag="ACG0BW_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258858.1"
                     /translation="MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMK
                     YIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQW
                     AMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
                     RAEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWL
                     LVLDCTDNVLILLPKVQPSKLMVLNCYDNCIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISAPRLPPKLIIYYCGENQF
                     KTVPVPQPRSLKVFSCNGNPWDKDNLPTLLKAVEGLKNQEGLEELLDFLHKECQMKLE
                     KLEKLEDLDDLMVLELLDDPVLLERVKVQEDLELLDQQLGLLSLERQQDSQPVNQHSE
                     HEPESASRVKRDLSEVEFESTMKRKRFM"
     gene            18694..18846
                     /locus_tag="ACG0BW_001891"
     CDS             18694..18846
                     /locus_tag="ACG0BW_001891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258859.1"
                     /translation="MVDGDTGNAALVILHLLQSEEAITLTFLSRCFELIGQSLQWFAI
                     HNLTFF"
     gene            complement(18948..20684)
                     /gene="cvrA"
                     /locus_tag="ACG0BW_001892"
     CDS             complement(18948..20684)
                     /gene="cvrA"
                     /locus_tag="ACG0BW_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFK7258860.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(20779..21693)
                     /gene="ldcA"
                     /locus_tag="ACG0BW_001893"
     CDS             complement(20779..21693)
                     /gene="ldcA"
                     /locus_tag="ACG0BW_001893"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFK7258861.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEPRTVT
                     LPLGAQAILTNTREGTQLTISGHPVLKM"
     gene            21793..22404
                     /gene="emtA"
                     /locus_tag="ACG0BW_001894"
     CDS             21793..22404
                     /gene="emtA"
                     /locus_tag="ACG0BW_001894"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFK7258862.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(22406..23140)
                     /gene="ycgR"
                     /locus_tag="ACG0BW_001895"
     CDS             complement(22406..23140)
                     /gene="ycgR"
                     /locus_tag="ACG0BW_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFK7258863.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITVETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            23341..23595
                     /gene="ymgE"
                     /locus_tag="ACG0BW_001896"
     CDS             23341..23595
                     /gene="ymgE"
                     /locus_tag="ACG0BW_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFK7258864.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(23645..25615)
                     /locus_tag="ACG0BW_001897"
     CDS             complement(23645..25615)
                     /locus_tag="ACG0BW_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFK7258865.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTITAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(25641..26495)
                     /gene="modD"
                     /locus_tag="ACG0BW_001898"
     CDS             complement(25641..26495)
                     /gene="modD"
                     /locus_tag="ACG0BW_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFK7258866.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLTQMLALLRERYPDGNIACTRKAIPGTRLLASQAILTAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(26492..27304)
                     /locus_tag="ACG0BW_001899"
     CDS             complement(26492..27304)
                     /locus_tag="ACG0BW_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFK7258867.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLKVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(27314..28072)
                     /locus_tag="ACG0BW_001900"
     CDS             complement(27314..28072)
                     /locus_tag="ACG0BW_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7258868.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHSQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLTLIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            complement(28069..29049)
                     /locus_tag="ACG0BW_001901"
     CDS             complement(28069..29049)
                     /locus_tag="ACG0BW_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFK7258869.1"
                     /translation="MRTVNGCILLLAVISITFAAVSGAYHLDIQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(29049..30071)
                     /locus_tag="ACG0BW_001902"
     CDS             complement(29049..30071)
                     /locus_tag="ACG0BW_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFK7258870.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISLARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRTAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQNYAQLFWHCPLSDADY
                     QKLVAS"
BASE COUNT         7494 a         7374 c         7704 g         7508 t
ORIGIN      
        1 tattgtgtga tttttatgtg agcagaaaat atttatcagc aatgattaca ttagtcattt
       61 tattttgtcg acggcctcat tgtcgaaaga taagcgtaca acagtattat cagaaaagag
      121 tgatttttta tccaactaca cttcagcgca ctgcgtttaa aaaatgcctc tttcttatgc
      181 gggatatcat catttcatca tgatgtcttt gatgagcggt gaacacaata cacttgcgct
      241 gtctcttcag gatcaatccc ctggtctggt gcctgcggga tgatgttgca gcagagctaa
      301 ggtcacttag acgctactat catttatcca atggcatgga atcgaaatca gtcgataccc
      361 gcagtatata tcgtgaactg ggcgcaacgc tgagttacaa catgcgtctg gggaacggca
      421 tggaagttga gccgtggctg aaggcggctg tgcgcaaaga atttgtcgat gataaccggg
      481 tgaaagtgaa taatgacggt aattttgtca atgatttgtc gggcagacgt ggaatatatc
      541 aggcaggtat taaagcctca ttcagcagtt cgttaagcgg gcatctcggg gtggggtata
      601 gccatggtgc cggtgtggaa tccccgtgga acgcggtggc tggtgcgaac tggtcgttct
      661 gaccatcaac gattaaactg cgcttcggcg cagttttcgt ttacagggtg ttaaaaggaa
      721 aaattctgcg gcaaaaaaag cccgccagtt acggcgggaa acctcatcct atgggagaac
      781 aatgaataat gaaattgcgg ggttatcatc acccagtata cccatactaa taataaggtt
      841 atttactcag ccaggtataa acaatttgat ctgtgtgggg ggaacggcat taaggtgtaa
      901 aggggggaag caatgagtag catcagcaag aatactcgcc gctcttatca aacgtggatg
      961 agggggatga aaaacgcaag gcagagatga ctctgccttg aagagaaatg cgcttttaca
     1021 gcgggcctat ttcagctctt ctgcttccgg caaggtcacg ttcagctcaa gaatagaaat
     1081 atcgccatct ttttgctcaa gctgtacggt taccatctca ggatcaattt gtacgtattt
     1141 acaaatgacc tcaagaatat ctttacgcaa ctgcggcaga taatgcggtt ctgcatcgct
     1201 gcgacggcgt tcagcaacaa taatctgcag ccgttctttt gcaatgttgg ccgtgttttt
     1261 cttccgcgag agaaagaaat cgagtaatgc cataacttat cctccgaaca agcgtttgag
     1321 gaagcctttc ttctcttctt caatgaagcg gaaaggacgt tcttctccca acagacgttc
     1381 tacggtatct gcgtaggctt tacccgcatc ggcgttaatg tcgagaatga ccggttcacc
     1441 ctggttagag gcgcgcaata ctgattgatc ctctgggatc acgccgacga gtttgatgcg
     1501 caggatctcc agcacatctt ccatgctcag catgtcacct ctgcttacgc ggcctgggtt
     1561 atagcgcgtt aacagcaggt gctctttaat aggctcttcg ccattttctg cgcggcgtga
     1621 tttcgacgcc agaatgccta aaatacggtc agagtcgcgt actgaggaga cttccgggtt
     1681 ggtggtaata atggcttcgt ctgcaaaata gagtgccatt agcgcaccgg tctcaatccc
     1741 tgccggggag tcgcaaacaa taaattcaaa gtccatcgct ttcagatcat caagaacttt
     1801 ggcgacccct tcacgggtga gggcatcttt atcgcgagtt tgcgatgccg gaagaatata
     1861 gagattttca gtacgcttat ctttaattaa cgcctgattt agcgttgcat cgccctgaat
     1921 gacgttgacg aaatcgtaaa cgacccggcg ttcgcaaccc ataatcaggt cgagattacg
     1981 caggccgata tcaaaatcta tcacgacagt tttctttccc ttctgggcca aaccagtggc
     2041 gatggccgcg ctggaggttg tcttaccaac accccctttg cccgaagtaa caacaataat
     2101 gcgtgccata gaaattcctt gttaaaaagg gatcaattta acggttgaac ggtcaaagcg
     2161 ttttcgacta actgcagtcg cgccgctttg ccataaaatt ctgctgggat ttgatcactc
     2221 agccagtatt cacctgcgat ggacaccaat tccgccatca ggttcgtgca aaatatttgc
     2281 gtttcccggt caccacttgc ccctgccagc gcacgaccgc gcatcatgcc atagacatga
     2341 atgttaccat cggcaatcaa ttcggcccca gcgctaacgt ggcttgtaac aatcagatca
     2401 cattgtggag cataaatacg ctgaccggaa cgcaccgggg tatctattaa acgcgttttt
     2461 gtgaccggcg ttgtattttg cgctggagcc tgcggtgcgg gagctggacg cggcgctttt
     2521 tcctttcctt ccgtcaggat aggcagcccc attttttcaa tttcggcttt aagttgcgca
     2581 tctttgcagc cacttacgcc aataacccgc aaaccggttg ccgaaaccgc cttatgcatc
     2641 gctgaccagt ttaccgggtc ttccagtgca ctgacgttga gtacaacggg ggcatgtttt
     2701 aaaaatgcgg gggcctgagc gattttgtct tccagcgcct gatggataac cttaggttct
     2761 gcctcatgca gatgaaccac agataaagtg aagctactgc ctttaagctc gattggcgtg
     2821 tttgacatcc tggccttact caattagcta ttaatcatcg ccagcgcgcg atgatgttcc
     2881 gaagactata aggcatgtta tagtctggat tatattgagg caagtcaccc tcccatttat
     2941 tcagagtaaa agtctattct gtgataaatg gcgctgattc atagcttaaa aaatatcctt
     3001 gtcaatcaac ccattgccgt cgtacttttg attgttctta tttacgcttc ctttttccgc
     3061 accctaatcg taacagcatc ttcagacctg tttaaatatc cagtattgag gcgagtgtat
     3121 tttttcacca cgccaggctt tcttaacttt ccattttttg cggcatcatt aaagaacaga
     3181 tacccgttat cttaatatcc gccacagaat tgctctatca tagaaaccgc agaagacgtc
     3241 tgatacttct gtcaggtcat cgtaactcgt ccaggtcagc ggttttgatt acggattgat
     3301 gatgaaatga gcataagtgt tgcacggttg gattatcagg atgtgctatg aatatgatgc
     3361 gtattattta tatcggattg tcaggtgtgg ggatgatgtt ctcatcaatg gcgtctggac
     3421 atgacgctgg cggacttcaa tctcctgcgt gtggcgttgt ttgcgacccg tatatttgtg
     3481 ttaactcaga tggcatttcc ccagagttaa caaggaaata tctcggcgaa aaagccgctg
     3541 aaaacttaca atcagtacaa ggctacgatc ccagcgaatt tacattcgct aacggtgtat
     3601 tttgcgatgt taaagaaaaa ttatgtcgtg atgatcgtta ttttggtgtg gatggtaagc
     3661 ggagtggaaa aatcaatcaa accaccacaa agatgttatt tatgtgtcgt gagtgatgtc
     3721 ttgtttacat tatgttctat tcgtcattaa cctcttgagc gacagagagg agtggggcgt
     3781 taacgtttat gacggggaga gtccccgtcg gttgacattt acttaatttg aatatcgacg
     3841 ttatattttt tagttttgtt tttacgggcg tcgtttcgag tttgcagcac tcgcagctca
     3901 tatttgccag aagcagggag tgagtattgt ccgtgactat cgagttctgg ggaatatctg
     3961 gataaatcaa cagaatcatc gattcctggc ccgaagaggt aagtatcagc gccttcatta
     4021 gagatactca catgcacttt ctgacctttt ttggcataga aggtatatgt atcgtaatcg
     4081 tatcccttta tttcgccgga gtactgagcg ctgctatggc cttttctgaa ctcaacattg
     4141 acattcttac ccgcagcaaa gctcgtggag gttagcagtg caagcaataa tacagcctta
     4201 ctgatgccct tgattttcat aggtagtctc ctgttcagaa tgaggacgat gaactgttat
     4261 aatataacaa ttcatcacag gagaattcca gtggctttcc tgcgttcggt taaatcccgg
     4321 cgcggcattt ttgccggggg atcacttatt aagggcgcat ggactgaaca aaccgcagaa
     4381 atctacagct ggctggcttg tggcgcgggt ttcattgatg ttagcttatg ccaaaacctg
     4441 gcatcttaaa gagtaaaagt atgttttgtg tgatttatcg aagcagcaag cgtgaccaga
     4501 cctatttata tgtcgaaaaa aaagacgatt tttcgcgtgt acctgaggaa ctgatgaaag
     4561 gttttggtca gcctcagtta gcgatgattc tgccgctgga tgggcggaag aaactggtca
     4621 atgccgatat tgaaaaggtt aaacaggcat taaccgagca aggttactat ttgcagttac
     4681 cgccaccacc cgaagatttg ctgaagcaac atctttccgt catggggcag aaaacacacg
     4741 acacgaacaa ataaccgata tccggcggtg gcattatctt tgtcggcgcg ggttttcata
     4801 tccacgataa ggtgagggga acgttatgta tcaacatcac aactggcaag gtgcgctgct
     4861 ggattatccg gtgagtaaag tagtctgtgt tggcagtaac tatgccaaac atattaaaga
     4921 gatgggtagc gcagtgcccg aagagccagt gctgtttatt aaaccagaaa cggcactgtg
     4981 cgatctgcgg cagccattgg cgatcccatc cgatttcggt tcagttcatc atgaagtcga
     5041 actggcggta ttgattggcg cgacgctgcg tcaggctacg gaagagcatg tccgtaaagc
     5101 cattgccggt tacggcgtgg cgctcgatct gacattgcgt gatgttcagg gaaaaatgaa
     5161 gaaagccggg cagccgtggg aaaaggccaa agcgtttgat aactcttgtc cgctttccgg
     5221 gtttattccc gcggcggaat tcaccggcga tccgcaaaat acaacgctgg gcctgagcgt
     5281 aaacggcgaa caacgccagc aaggtacgac tgcggacatg atccataaaa tcgttccgct
     5341 gatcgcttat atgagcaagt tttttaccct caaggccggt gacgttgtgt tgacaggcac
     5401 gcctgatggc gtcggcccgt tgcaaagcgg tgatgagctg acagtcactt tcgatgggca
     5461 ttctttgaca actcgcgttt tgtaatactt tttgccgcct gaaagcggcg gcaaaacttg
     5521 catcgctgtg ccagactggt tataaggtgc gttttaacgt aatggcggaa cacctgatga
     5581 gcgatgtacc tttctggcaa agtaaaaccc tggacgaaat gagcgatgcg gaatgggagt
     5641 cgttgtgtga tggttgcggt cagtgttgcc tgcataaact gatggatgaa gacaccgacg
     5701 aaatctactt cactaacgtc gcctgtcgcc agctcaatat taaaacctgt caatgtcgga
     5761 actacgaacg tcgtttcgag tttgaacccg actgcattaa attaacccgt gaaaatctgc
     5821 caacattcga atggctgcca atgacctgcg cttatcgttt gctggcggaa ggtaaagatt
     5881 tacctgcgtg gcatccgctg cttactggtt cgaaagcggc aatgcatggt gaacgtatct
     5941 ctgtgcgtca tatcgcagtg aaagaatcag aagtgattga ctggcaggat catatcttaa
     6001 ataaacctga ctgggcgcag tgataaattt aatagatttt gatgggggct ttcttaatat
     6061 acgtctggat gctcgccatt tcgtagccct gtttttgaaa gcgctctgtc ataatgagag
     6121 ttacccgata ccttattatc aacaggtaac tctcaatctt taaaaaacgg ctgtgttttt
     6181 aaatgtgata aactttatac cttgagtaca tggagagaat aatcgcttat cagacttcag
     6241 gtacctcaaa gagtgtcttc ttaccgtgtc ttttctgata ctcattacag gtgttaatca
     6301 tttttttggc cgcttctttt agcaaagaaa gcattaaatc atcataatca acgtagaatc
     6361 tggttgtttc agtttccgtt tttatctccc cgatggcggc tatttcggcg gttaatttca
     6421 atttggcggc atcgatatct ttattcgctt gtttaaccgt gttagacagg gtggtaaaga
     6481 aactctgcac agattttaac ttgttcttca attctggaat cagtttccct tcaactacgc
     6541 ccgcagcaat agaataggaa atgattaatc caaatggacc ggcgacgaca ccggctgcgg
     6601 caccggcata cgcttccttc ctgattttat ctacctgtga ctggaaatag ctgctttttt
     6661 ctgaaaaatc attggttaac tggctatcta acgccagcag tttcccggaa gcgttgttga
     6721 aactttgtga gcttaccagc agggattttt gcgcttcatt cagcttcgtg atgccgtcat
     6781 ccagtacctt aatgagaatg tctttctggg cggatgcttt cttctcattg tactcatcaa
     6841 atagcaaaat atacgctgcg agcaattgcg tcgcaacacc acaccattca tacaccgttt
     6901 gggttgcttc aaaatactta tcctggctat ccataagtaa ggttttaata tcgccaacta
     6961 aaacggaggc tgcctgtgaa tactcctgtt taaagcgact taactctttt atggtttcat
     7021 cgaaggtctg ccaggggatg acctgatcga gatatttatt ataaaggtct aatgctccat
     7081 ctgcggtttc gattgcgttt ttaactacct ctaccgtttt atctgcaacg atttcagtca
     7141 taatcattcg cctctttaaa tatataaatt gtaatgaaac tcctgtttta caactattaa
     7201 tgaattttac ttcatctaat tcatagttag ccgggcgaga tgcgtcaatg tctttatttc
     7261 tattaatatg ataaatatca aacaatgttt aatgtcatta tggcgaatgc ttctattcta
     7321 tttttagccg ggtgatattt ttcatttctg ctggatgagc gtcgtcgcca gaaggccgcg
     7381 tgagcacagg ataagagaac gaaaaatcag cagtctatgc agcgacaaat attgatagcc
     7441 tgaatcagta ttgatctgct ggcaagaaca gactactgta tataaaaaca gtataacttc
     7501 aggcagatta ttatgttgtt tatcaagcct gcggatctcc gcgaaattgt gacttttccg
     7561 ctatttagcg atcttgttca gtgtggcttt ccttcaccgg ccgcagatta cgttgaacag
     7621 cgcatcgatc tgaatcaact gttgatccag catcccagcg cgacttactt cgtcaaagca
     7681 agtggtgatt ctatgattga cggtgggatt agtgacggtg atttactgat tgtcgatagc
     7741 gctattaccg ccagccatgg tgatattgtc atcgctgctg ttgacggcga gtttacggtg
     7801 aaaaaattgc aactacgccc gacagtgcag cttattccca tgaacagtgc atattcaccc
     7861 attaccatca gtagcgaaga tacgctggat gtctttggtg tggtgatcca cgtcgttaag
     7921 gcgatgcgct gatgtttgcc ctctgtgatg taaacgcgtt ttatgccagc tgtgagacgg
     7981 tgtttcgccc tgatttatgg ggtaaaccag tggttgtgct atcgaataat gacggttgcg
     8041 ttatcgcccg aaacgctgag gcaaaggcgc tgggtgttaa aatgggcgat ccctggttca
     8101 aacaaaaaga tctgtttcgt cgctgtggcg tggtttgctt tagcagcaat tatgagcttt
     8161 acgcagacat gagcaatcgg gtgatgtcga cgctggaaga gctatcgccc cgcgtcgaga
     8221 tttacagtat tgatgaggca ttctgcgatc tgaccggtgt gcgtaattgt cgcgatctga
     8281 ctgatttggg cagagaaatt cgcgcaacgg tactacaacg tacccatctt actgttggtg
     8341 tggggatcgc ccagaccaaa acgctggcta agcttgccaa tcatgcggca aaaaaatggc
     8401 agcggcagac gggtggggtg gtggatttat caaatctgga acgccagcgt aaattaatgt
     8461 ctgctctccc cgtggatgaa gtctggggga ttggacggcg gatcagcaaa aaactggacg
     8521 cgatggggat caaaaccgtt ctcgatttgg cggatacaga tatccggttt atccgtaaac
     8581 attttaatgt cgtgctcgaa agaacggtgc gtgaactgcg cggtgaaccc tgtttgcaac
     8641 tggaagagtt tgcaccgaca aagcaggaaa ttatctgttc ccgttcgttt ggtgaacgca
     8701 tcacggatta tacgtcgatg cggcaggcca tttgtagtta cgctgcccgg gcggcggaaa
     8761 aacttcgcag tgagcatcaa tattgtcggt ttatttccac gtttattaag acgtcaccat
     8821 ttgcgctcaa tgaaccttat tacggcaata gcgcgtcggt aaaactgctg acacccactc
     8881 aggacagcag ggatatcatt aacgccgcta cgcgatctct ggatgccatc tggcaagcgg
     8941 gccatcgcta ccaaaaagcg ggcgtgatgc tgggagattt cttcagtcag ggagtcgcgc
     9001 agctcaattt attcgatgac aacgcaccgc gccccgggag tgagcaattg atggcggtaa
     9061 tggatacgct aaatgctaaa gagggcagag gaacactcta ttttgctggg caggggatcc
     9121 agcaacaatg gcagatgaag cgtgccatgc tttcaccacg ttatacaacg cgaagttctg
     9181 atttactgcg ggtcaaataa acatagtggc aggaaaaaag cgctcccgca ggagcgctga
     9241 atggattagc gaccgaacag atcacgtttt ttcgctttaa acggctggga aatcaccacc
     9301 agcaccgcga caatcaggta agcgataaaa ataccgagca accactgcgg catttccaga
     9361 cctaaaaaat cccactgacg ctcggcgcaa tcgccagagg cgacaaacac ttgcggcacc
     9421 catttatcca gcggcagcca ttccgggaaa cgaaccataa aatcacaggt cgcaaacggc
     9481 gaaggataga gctgaagcat ggtgtgctcg taagttaact gcacaccgcg gaacgcacta
     9541 tacaaccaga taaccatcgc tacataacgc agcggagttt tcggggcgat cgcgccaatc
     9601 agcgcagcac ccagaacgcc gaataacgcg cagcgttcat aaatacagag cacgcaaggt
     9661 ttcagtaaca tcacatgctg gaaccacagc gccgtcagtt ccagtgccag agcagtaaac
     9721 gccatcaaca gccacgcgcc ccggccttgt gaacattggt tcaaaaatcg caacataatc
     9781 atttccctgc aatatgcata gagtgcgcag tttaaaccaa ttcattcgct gcgccaccag
     9841 gaggcagcaa aaataggacg caattggaca attatcgggc gaaaaggcaa accgggcatt
     9901 tatatgcccg gtaagttgtt atcaaagcgt tgctatccag cccatttgca taaaccattc
     9961 ggttacaggg gcaagcgtaa actcgacgca aagtagtccg acgagtgtca gtacgagggt
    10021 gtaaggcagg gccatccaca ccatgcggcc ataagagagg cgaatcaatg gcgcgagtgc
    10081 agaggtcagc aggaacagga acgcagcctg accgttcggc gtagcgacgg agggcagatt
    10141 ggtaccggta ttaatggcga ccgccagcag ctcgtattgc ttcaacgtga tagcgccgct
    10201 ttccattgcc gctttcgctt cgttgatata aatcgtcccc acgaagacgt tatccgaaat
    10261 ggatgacagc agaccgttga aaatatagaa cagcgacagc tgagcatgtt ccgatgcctg
    10321 caacacaaac tgaataatcg gcgaaaacag ttgttggtcg ataatcaccg cgactaccga
    10381 gaaaaacacc gtcaacagtg cggtgaatgg cagagattcg gtgaaggctt taccgatagc
    10441 atgttcatcg gtgacaccgg tcaatgatgt cgccagaata atgaccgaca aaccaatcaa
    10501 gccaacttcc gccagatgca acgccagcgc agtcaccagc cagacgccaa taatcgcctg
    10561 gacaatcaga cggattttat cctgacgggt acgctggtgg cggctttgat cgtcaaactg
    10621 ttgcaacact tcgcggactt tctccggcag cgtttcaccg tagccaaacc aacgcagctt
    10681 ctctaccagc aggcaggtta acaggccaca aatcagaacc ggaacggtca ccggcgacat
    10741 gcgcaggaag aaatcgccaa aatgccagcc agccgcttta gcgatgatca ggttctgtgg
    10801 ttcgcccacc atggtcatca cgccgcctaa tgcagtgccg acaccggcat gcatcatcag
    10861 gctacgcaga aagccacgga actgttccag aaccactttg taatgcttgt cgatatgact
    10921 atcgtcttgc aggtcggtat cttcggtacg ggaagaggct acgcggtgat aaatcccata
    10981 aaaaccgact gcaacgctga tcaccaccgc cacgacggtt aaggcatcga ggaacgcgga
    11041 gaggaacgcc gccgccacgc aaaaagagag cgacagcagc attttggagc gaatgcttaa
    11101 cagcaaacgg gtaaatatga acagcaatag ctgtttcata aaatagatac ccgccaccat
    11161 aaacatcagt aacagcaaga cttcaagatt tgccgccacc tcttcacgga cgtgttccgc
    11221 gctggtcatg ccgatgaata ccgcttcgat agccaacaga ccaccgggga gcagcgggta
    11281 gcatttcagg gccatcgcca gagtgaaaat aaattccgct accagcaacc agcccgcgac
    11341 gaaagggctg atgaggaaaa ttaacggatt tacgattaag aaaatgatga gggcgagttt
    11401 gtaccagtcg ggggactggc ccaaaaaatt gcgccatagc gcgcggcccc aggagatctc
    11461 catgatggtt tcccttacct tacaaataat caatgatgtt tttatgttta aacgcaaagc
    11521 ttaacggtca ggcaggagtg aggcaagtct tgatagtcaa ggggaaagag atgcggaaaa
    11581 tgaagccttg atcccttttt tcttcttttt gtctgctatc agcgtagtta gccctctggt
    11641 atgatgagtc caactttgtt ttgctgtgtt atggaaatct cactatggtc attaaggcgc
    11701 aaagcccggc aggtttcgcg gaagagtaca ttattgaaag tatctggaat aaccgcttcc
    11761 ctcccgggac tattttgccc gcagaacgtg aactttcaga attaattggc gtaacgcgta
    11821 ctacgttacg tgaagtgtta cagcgtctgg cacgagatgg ctggttgacc attcaacatg
    11881 gcaagccgac gaaggtgaat aatttctggg aaacttccgg tttaaatatc cttgaaacac
    11941 tggcgcgact ggatcacgaa agtgtgccgc agcttattga taatttgctg tcggtgcgta
    12001 ccaatatttc cactattttt attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg
    12061 aggtgctggc taccgctaat gaagtggccg atcacgccga tgcctttgcc gagctggatt
    12121 acaacatatt ccgcggtctg gcgtttgctt ccggcaaccc gatttacggt ctgatcctta
    12181 acgggatgaa aggactgtat acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc
    12241 gcagtctggc gctgggcttc taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg
    12301 atcaggtata cgaaacagtg cgtcgctatg ggcatgagag tggcgagatt tggcaccgga
    12361 tgcagaaaaa tctgccgggt gatttagcca ttcaggggcg ataatccctt ccgtttaaaa
    12421 agcaaacccc tcaaacgagg ggtttttgtt gtttttacag atttcccatt ctcggcgggc
    12481 aacgttccag caactcgacg ctgccgtctt cgttttgctg ttccagcatt acatcaaatc
    12541 cccacaggcg atgcacatgc ttcagtactt ctttgcgccc ccgatccagc ggtgcgcgat
    12601 tatgtggaat gtaacgcagc gtcagcgaac ggtcgccgcg caaatccacg ttccagatct
    12661 gaatattcgg ctccagatta cttaaattat attgagacga taaccggtta cggatctccc
    12721 gataaccttc ttcattatga atagcggaaa tctccagata attatgccga tcgtcatcca
    12781 gcacggtgaa gaagcggaaa tcacgcatca ctttcggtga caggaactgg ctgataaaac
    12841 tctcatcttt gaaatcacgc atcgcgaaat gcagcgtttc cagccagtcg gaaccggcga
    12901 tatccgggaa ccagtatttg tcttcttccg ttggcgactg acaaatccgt ttaatatcct
    12961 ggaacatggc gaacccgagg gcatacgggt tgatgccgct gtaccacggg ctgttatagg
    13021 gcggctggaa gaccacattg gtgtggctgt gcaaaaactc cagcataaaa cgttccgtta
    13081 ctttcccttc atcatacaga tggttaagga tggtgtagtg ccagaaggtc gcccagcctt
    13141 cgttcatcac ctgagtctgt ttttgcggat aaaaatactg gctcacctta cgcacaatac
    13201 gcagaatttc acgctgccat gattccagca gtggcgcatt tttctccata aaatagagca
    13261 ggttttcttg tggctcggac ggatagcggc gcgcttcagc aaccgttttc tcttcctcgc
    13321 gcttcggcag ggtacgccag agcatattga cctgactttg cagatactct tcgcgacttt
    13381 tctgccgggc tttctcttct tgcagcgaga ttttttgcgg gcgtttgtac cggtccacgc
    13441 cgtagttcat cagcgcgtgg cacgagtcca gaagccgttc tacttcatca acgccataac
    13501 gttcttcgca ctcggtaata tatttacggg caaaaatcag ataatcgaca atcgaactgg
    13561 cgtcggtcca gctacggaat aagtaattgt ttttgaagaa agagttatgc ccatagcagg
    13621 cgtgtgccat caccagcgct tgcatggtaa tggtgttttc ttccatcagg taagcgatac
    13681 acgggttaga gttaatgacg atttcatagg ccagtccttg ctgaccgtgc ttatacagcc
    13741 gttcagtctc gataaacttt ttaccgaatg accagtgcgg atagttaatg ggcatgccga
    13801 cgctggagta ggcatccatc atctgttctg aggttatcac ttcaatctgg tgcgggtagg
    13861 tatccagccg gtagagtttc gccacccggt ctatctctgc cagataaaca tccagcaggt
    13921 cgaacgtcca gtcgggtcca tcgctcaaac gtgtggtgtc cttattcata gaatcgatcg
    13981 tcgccatacg cgcacctcat tgttgtcggc gctctctgtg tggagcacct catttcaagc
    14041 atagaacacc tgttaaaaac cgcgtcgccg gagaattttt ttctttgcga tttcttatta
    14101 tcagagtgcc actaatccgc ttctgaacgg aattttatgc tggataaaaa gggcgttcag
    14161 caggagatac taaagacgcc atattgccgc agagtcaggg agatgtgagc cagctcacca
    14221 taaaaaagcc gtatgttgaa taatattttc aactgagtta tcaagatgtg attagattat
    14281 tattctttta ctgtatctac cgttatcgga gtggctatgc gagttgtcat actgggaagt
    14341 ggtgtggtag gcgttgccag cgcctggtac ttaaatcagg caggacatga ggtcaccgtc
    14401 attgatcggg agccgggggc cgccctggaa accagtgccg cgaatgccgg gcaaatctcc
    14461 cccggatatg cagcaccgtg ggcggcacca ggtgtgcctt taaaagcgat taaatggatg
    14521 ttccagcgcc atgcgccgct ggcggttcgt ctcgacggta cgcagttcca gttgaaatgg
    14581 atgtggcaaa tgttacgtaa ctgcgacacc agccactaca tggaaaacaa agggcggatg
    14641 gtgcgtctgg cggaatacag ccgtgattgc ctgaaagcat tgcgcgccga aaccaatatt
    14701 cagtatgaag ggcgtcaggg tgggacgctg caactgttcc gtaccgaaca acagtatgaa
    14761 aatgcgaccc gcgatatcgc cgtgctggaa gatgccggcg taccgtacca gctgctggaa
    14821 tccagccgtc tggcggaagt ggagcccgcg ctggcagaag tggcgcacaa actgacgggg
    14881 gggctccagc tgcccaatga tgaaaccgga gactgccagc tgtttaccca gaatctggcg
    14941 cggatggcgg agcaggcggg ggttaaattc cgctttaata cgcccgttga ccaactgctt
    15001 tgcgacggcg agcaaatcta cggcgtgaag tgtggcgatg aagtgattaa ggccgatgcg
    15061 tatgtgatgg cgtttggttc ttactcgacg gcgatgctca aaggcatagt tgatattccg
    15121 gtgtatcctt tgaaaggcta ctcgctgacc attcccattg cgcaggaaga tggtgcgccg
    15181 gtatccacca ttcttgatga aacctacaaa atcgccatta cccgtttcga taaccgcatt
    15241 cgtgttggcg gaatggcgga aattgttggt tttaataccg agctgttgca accgcgtcgt
    15301 gaaacgctgg agatggtggt tcgcgatctc tatccacgcg gcggtcatgt cgagcaggcg
    15361 actttctgga ctggtctgcg cccgatgacg ccagacggca cgccggttgt cgggcgtaca
    15421 cgctttaaaa atctgtggct gaataccggt cacggcacgc tcggctggac gatggcttgc
    15481 ggttccggtc agttgttaag cgatctgctc tctggtcgca cgccagcgat cccgtatgaa
    15541 gatctaagcg tagcgcgcta cagccgtgga tttacgccat cacgcccggg ccatttacat
    15601 ggcgcacaca gctaaggaaa cgagatgacc cgtccgatac aggccagcct cgatctgcag
    15661 gcattaaaac agaatctgtc cattgtccgc caggccgcgc cgcacgcgcg cgtctggtcg
    15721 gtggtaaaag cgaacgctta cgggcacggt attgagcgta tctggagcgc gctcggggcc
    15781 accgatggct ttgcattact taacctggaa gaggcaataa cgttacgtga gcgcggctgg
    15841 aaaggaccga tcctgatgct ggaaggattt ttccatgctc aggatctgga gatttatgac
    15901 cagcaccgcc tgaccacctg cgtacacagc aactggcagc tcaaagcact gcaaaatgca
    15961 cggctaaaag ccccgttgga tatttatctt aaagtgaaca gtgggatgaa tcggttgggc
    16021 ttccagcccg atcgcgtgct taccgtctgg cagcagttgc gggcgatggc gaatgttggc
    16081 gaaatgaccc tgatgtcgca ttttgccgag gcggaacatc ctgatggaat ttccagcgcg
    16141 atggcgcgta ttgagcaggc ggcggaaggg ctggagtgtc ggcgttcgtt gtccaattcg
    16201 gcggcgactc tgtggcaccc ggaagcgcat tttgactggg ttcggcctgg cattattttg
    16261 tatggcgctt cgccgtccgg tcagtggcgt gatatcgcca ataccggatt acgtccggtg
    16321 atgacgctaa gcagcgagat tattggtgtc cagacgctaa aagcgggcga gcgtgtgggc
    16381 tacggcggtc gctatactgc gcgcgatgaa cagcgaatcg gcattgtcgc cgcaggatac
    16441 gccgacggtt atccgcgcca cgcgcctacc ggtacccctg ttttagtgga cggcgtgcgc
    16501 accatgacgg tggggaccgt ctcgatggat atgctggcgg tcgatttaac gccttgcccg
    16561 caggcgggta ttggtacgcc ggttgagctg tggggcaagg agatcaaaat tgatgatgtc
    16621 gccgccgctg ccggaacggt gggctatgag ttgatgtgcg cgctggcgtt acgtgtcccg
    16681 gttgtgacag tgtaacttgt tgtaagccgg atcggaggca gcgtcttctg gttgcgaaaa
    16741 aatcatccat ccggctggtc agcaactgta gttgttgatg tgacagagcc atttcccatg
    16801 atagtgtcca ttacaatgat ggacactatt tccccggaac ctgagttcac cgcacaggcg
    16861 ttctacataa aacgcttacg tttcattgtt gactcgaact cgacttcaga taaatcacgc
    16921 ttcacccttg atgcagattc gggttcatgt tcagaatgtt gattaacagg ctgcgagtcc
    16981 tgttgtcttt ccagactcaa caggcccaac tgctgatcca ggagctccag gtcctcctgt
    17041 accttcacgc gctccaggag taccgggtca tccaggagct ccagaaccat aaggtcatcc
    17101 agatcctcca gcttctccaa cttctccagc ttcatctggc actccttgtg caaaaagtcc
    17161 aaaagctcct ccagaccctc ctggtttttc aggccctcga cggctttgag cagcgtcggt
    17221 aaattgtctt tgtcccacgg gttaccatta cagctaaaca ccttcaggct tcggggctgc
    17281 ggcaccggta ccgttttaaa ctggttttct ccacagtagt atatgattag tttgggcggc
    17341 agccgtggtg cagagatcaa atcgttacta gaacagtcaa atactttcag gcgagtcaag
    17401 ttctccagca tctcatcagg tattgagtta atattgttgc cggcacagga tagtttatac
    17461 aggtactggg gcatcgacgg cggtaaaaat tgcaagaagt tttcagaaca gttaatcact
    17521 ctcaggttag tcgacagctc aggcagccag ataatacagt tgtcatagca attcagtacc
    17581 atcagttttg agggctgcac cttcggtaac aagatcaaga cgttatctgt acagtcaagc
    17641 accagcagcc actttggcat caccttcggt aacgcggtca acacgttttt actgcagtaa
    17701 aattcaatca gatgtggagg caaattttca gggagtgaag tcaatccaag gttgctaaga
    17761 ttgagcatac tccgtcttgt aatgagacat tccttcattc tgcgcaatgc ttctgcccgg
    17821 ttttcaatcc ggcttgctgg caggttttct tccaggatat tttccttgat ttcttccaga
    17881 ctttctttca ggtcttcttg gggaatttct gactgtttgt tttgctggtt ttcttccagg
    17941 atttcgtaca ggtttttttg caggttttct tgcaaaccat cccgctccat agcccattgg
    18001 caccattcgg cgaccatcat gaactcgtta tgttgcggat tcggattcag gcgctccaac
    18061 gtgatgatgg tattgtctac catatgtatt ttcacttcgc ccgcggtact gaggtcaatg
    18121 atctgccggt ggtcatcacc ccagctaaat atttgcgggc cgatatactt catgttccgc
    18181 aaaatttcat tcttgagttc caccggcagg aagaactgcc ctggtgggcg tacgcctggg
    18241 ttaacggaag atgggggaat gccgggggcg aatgggcgga tgtctttaat gctggccata
    18301 atgctacata tccttgaaat aaatatcctc tggcagcgcc ggagacgtga atgtggtcac
    18361 cgcaaaggtg ccacaggacg tcatttgcgc tgatataact actcatcgtt gactttttcc
    18421 cttggtcaga aatgccgtgt cgggtctggt acatgctgta ttacggccct ggcagctctg
    18481 acgtattaaa gaaaatacga aagcgaacga cccggaaata atcgttatta agcaaaggaa
    18541 taactataag aaatcgctca tgcatccggt gctacaggcg gatgaaacgc cactacagtt
    18601 actgagcagt ctgaaacgcg tattgtgtgg gctacgccag tgcggcaggt tgcgtggggg
    18661 aatatgttca ggcgatgtca ggctggggcc ctgatggttg acggggatac cggtaatgcg
    18721 gcgctggtta ttcttcacct gttgcagtca gaagaagcca ttacgctgac tttcttgagc
    18781 cggtgttttg agttgattgg ccagagcctg caatggtttg ccatccataa tttaaccttc
    18841 ttctgatgtt ggaatgtgca tatcaaaatc aggaaatgac aagtttatat gcaggctcag
    18901 gctgcgttcg caaagcatcg catccggcga tagatgctgt attaaattta agattcagct
    18961 tcctcttctg ccacccgtac gccaatcttc aacacttcat tatcttcttt ctcggccacc
    19021 gtccagatca ttccggcaaa ctctacctgg tcaccgacaa ccggtgccgc gcctaacaac
    19081 tgctggacaa tttcacccag cgtttgttgc ttatcacgat attctcgccc gtcttccagg
    19141 ccatatatca gcgccacatc tgcatatttg gcgctggctt cgagaatgaa gtcaccaaag
    19201 aagcgttgat ccagcgcgac cggcggcgac tggctgaaca gtttaccgag cgccgggaga
    19261 tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt gctgccggta
    19321 ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt tggcatatgc
    19381 agatcacgca gtgccgcgcc cacgcaccat ttatcggcac tcaactgata aacaaactgc
    19441 tcccacggat tttccggatg aatatccaga ccaacgcgtg acaccggacg tcccactggc
    19501 ggaacgacca ctttggcttt tttcgccgcc cacgagagtg atgttccctg caagagcagt
    19561 gaaaccagaa ccacaaagaa ggcgacatta aagaacagtc gcgcattctc cagccccgcc
    19621 atcatcggga acacggccag gatgattggc accgcgccgc gtaatcccac ccagctgata
    19681 aacacgcgct cacgcagatt aaaaccacgg aacggtagca atccggcaaa taccgaaaga
    19741 ggacgggcga agaaaatcat ccatgcggac aaaatgagcg ccggaatggc aataggcagc
    19801 agatcgcttg ggttaaccaa cagccccagc accaggaaca tggcgatttg cgccagccag
    19861 gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg attacccagc
    19921 agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt ggttaaagca
    19981 aaaattaaaa taccgccgct taatgccagc aatggatata aaccggcggg cagggcgatg
    20041 cgattaatca tttgcagcag taaataacca ccgccaagac cgatgacaat cccgaggcca
    20101 aattgctgca aaatatcgac cacgaacatc cagctgacac tgctttcatg ctgctggatc
    20161 atcgcaatca gggtaatcgt cagaaagacc gccattggat cattactgcc ggattcaatt
    20221 tccagcgtcg agccaacgcg ttcgttaagc cccttaccac ccagcagaga aaataccgcc
    20281 gcagcatcgg tggagccgac gatagcgccg attaataagc cttcaatcaa atcaagatta
    20341 aacagccacg ccgccatcat gccggttaaa ccagaggtga taagcacgcc cagcgtcgcc
    20401 agcgacagcg ccggtcctaa cgccacacga aaggaactgg cctgagtgcg catcccgccg
    20461 tcgagcaaaa taatcgccag cgccaggtta cttaccatgt aggcgaaggg gtaattatca
    20521 aacgggatac cgccgacgcc atcgactccc gccagcatgc cgatcgccag aaaaataacc
    20581 agaataggaa tgccaagacg ggaagaaaat gaactaagta atatactgct ggtgacaagg
    20641 atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg gttactcctg
    20701 attacgctgt ctcttatata aaccctacca tattagcggc agagacagcg ttttacttag
    20761 ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg tcaactgagt
    20821 gccttcccgg gtgttagtaa gaatggcctg tgcgcccaac ggcagcgtaa ccgtgcgtgg
    20881 ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac gcaaaaacgc
    20941 gtacactgac tccaggttgt aacctgcgtc ataatcattg ggcgtgctgc cgctaaagct
    21001 accgagaata atcgccttct gacgtggcaa aattccagca tgatagagct gcaacagcat
    21061 acgttcgacg cggaaaggat gctcgttaat atcttccagc accagaatac cgttttcgat
    21121 ttgcggcatc cacggtgtac caatcagtga aatcagcatc gcgagattgc ctccccacaa
    21181 cgtgccttcg gtctgacatg tcgggccttc cccttgccat tcaatggtga aggtttcatt
    21241 gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc agttcatccg cgccaaagtt
    21301 tgctaccagc atggggccgc taaaggtgat gacattgcca tgcgccagaa gaccgcactg
    21361 aatggcggta aaatcgctat gtccgcaaat gagcaacggg tcatgttgtt ggcgagccac
    21421 cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc cgcgtacagc
    21481 cagcacgatg gtgttggggg tggtcagtct ggcaagggaa ttgagatcct ccagacgctc
    21541 cgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt tattgacctg
    21601 atgccccgcg tcggtaaggc gctgaatacc acgcagcgcg gcgtgctgtt taatgcagta
    21661 acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac aacagaaacg
    21721 aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt ccaggctata
    21781 aggatagaag aagtgaaatt aagatggttt gcctttttga ttgtgttatt agcgggttgt
    21841 tcatcaaagc atgactacac gaacccgccg tggaacgcga aagttccggt gcaacgtgcg
    21901 atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga tccacaattg
    21961 atcacggcga ttatcgctat cgaatcgggt ggtaatccga acgcggtgag taaatcgaat
    22021 gccattggtt tgatgcagtt aaaagcttca acctccggac gtgatgttta tcgccgtatg
    22081 ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc cggagcgtaa tatttcaatg
    22141 ggggcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga agatccgaag
    22201 gtactgcaat atgcgctggt ggtgtcatat gctaacgggg caggtgcgct gctacggact
    22261 ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atctggatgc tgatgagttc
    22321 ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc cgcgctatat ctacaaactt
    22381 gagcaggcac tggacgcgat gtaaatcagt cgcgcacttt gtccgctttc tcccgggctt
    22441 cccgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg ctgacgttaa
    22501 gaaaacggaa gctcagacgg ggagtggtga tggtttcatt cttgccatca atcactttgc
    22561 gctcgctgat tgagattaac tgggcgtcaa agtgaaaaac accccattgc cccatgttga
    22621 cttcaatctg agcgaagcgc atgccttcgt gcaatccggc aggctttgct gtttccagta
    22681 atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta ctgttatccg
    22741 ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggagatgcgg aaatatcggc
    22801 gtcgttgtac aaaccacaag gtgggaggcg gtacggtaat aaatgccgga agctgcaagt
    22861 attcactctg ctgtagttgt tcaacagtaa attcgacttt cgcgccctgg gtttcgacgg
    22921 taatggtaat gtgctgcgcc tttaacacag cgttgttatc ttcggcttga ctgccgaaat
    22981 ccagcaccag tttatccggg gtaattgcca gtattttgct gatcagctgc ccgccattcc
    23041 agctgatacg caaaggaatt gcggctttgt gcaaatcgcg taacacgccc aggacggcta
    23101 acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac tcctgactga
    23161 caaactgtct ttggtttatc ggtcacagtt aacaaaactt aatacaaatg cgtgaatatt
    23221 ttttacatgt tgttcttaaa tcagccgaca tacgcccagc atcgattccg cccttgccta
    23281 tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga gggcctgaac
    23341 atgggaatta ttgcctggat tatttttggc ctgatagccg gcattatcgc caagctaatc
    23401 atgccggggc gtgatggtgg tggatttttc ctgacctgta ttctcgggat agtcggtgcg
    23461 gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccattag tggttttaat
    23521 ctgcacagct tcctggtggc ggtggtgggc gctatcctgg tgctgggcgt attccgcctc
    23581 ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc tatccgcttt
    23641 cacatcagaa cgtatactcg atacctgccc agaaattgcg accttcttca ataaaccctt
    23701 cactgtaggc atatttggta tcaaacaggt tattgaccga cgcattgacg ctgaagccat
    23761 gacctaaggt gtaatcggct cgaatgtggg tcaccgcaaa accggcggct ttttgtgaac
    23821 cgtcactgtt gctgtagctg gaggaacgcg cctcttccga cagcgttacg cttaacggct
    23881 cccacggttt gagagtcatc catgcggtta ttgtctgcgt tggcagatcg gttatcttgc
    23941 cgatgtcttt gcgtttggcg tcagcgtgga tcagggcgta gctcaatcct acatccagta
    24001 tattgctgat tttcccctta ataccggcat ccagaccgct gtaatccacc gtgccgctgt
    24061 tctgattttg aatggtatcg gcatcgatat tgtgcgagag gatggcatca ctcacccggt
    24121 tatagtaaac gctgacctca aacccccagt cgtgcgtgaa ggcaccattc caggttaaat
    24181 ccaccccgcg cgcgcgttcc ggtttgagct gcgggttaac aatcgctatc tggttatacg
    24241 caggtttgga cgtggtatag cgttctttca gcgtcggaaa gcgtgtgcgg tcatagtacg
    24301 aaagcgccag cgtgtcttca ttggcaaagt ggtatttccc catcacctgc cagttaaaag
    24361 ctgactgatt gttgtcgtca taatgggtga tgcttccatc tttctcatgt tttttcgctt
    24421 ctacgctatc gcgccagtca tagctgattc cagccacgac atcgacatta tcggcagcag
    24481 cccattgata ttcactggcg agcgaccagg tacgatcttc atagcgatcg taagcggcgt
    24541 gcggcgcacc tttttcccgg tgtacgtcat ctttccagtt aacggcaaac gacagcagat
    24601 cgttttcacg cacatcggct gccagttgta acccggcacc gtcgctgtaa tcggaataat
    24661 ggctgtagct gcctttttta tttttcaaat cagccagcga gttgtacatc atcagcgtat
    24721 tttcaaaggt gtcgcgatat agccgacttt tcagggtaaa acgatcgttt agttgggtcg
    24781 ttccctgata ataaaaactt tctttgtcat actctggcca ctgccagtag cgtgattttt
    24841 gaccactatt tccactgtat ggtgggttat ctttttcacc atcctgctta atgtaagtca
    24901 atgtgtattc atcgttttcg cgtggtgtaa aaccgagctt cacaatgccg cgtttatcat
    24961 cagccgatga attaatcatc ttgccgtgtt tgcctgcaat atcattattt acaccatgcg
    25021 gcaggccgag aaaatcctgc tttagctgac taccgctgac ttgcaaatac cccagatcgc
    25081 tgctggcggc aaatgaagca tgcatatcgt aggcattgtc ctggctacgg ctccatccct
    25141 ggcgatatcc cagatttgct tccagaggtt ttgttggctt ctgggtggtg atattaatgg
    25201 ctccgcccat ctgattaggc ccctgaagca gcgacgaata ccctttggaa acttcaactg
    25261 cccccaaatt gttggtcaga atccgcgcca gatcgaggtt gccgtcatag ggaacataaa
    25321 tgggcacacc gtcgaaatag accggcacct gacgactatc aaagccacga acttttacct
    25381 gttcttcgtt gcggctgcct gacttttgca gcaccacgcc ggggacgaca cttaatgcct
    25441 gagcgacatt ctgtttatcc agggccttca tggtgggttg atcgagaacg gtggtcgtcg
    25501 tcgatgatac cggactggac catacggtca gcgtgtcgct ctcctgagcc gctacggcct
    25561 gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt ctcatagaat
    25621 aaattccgtt taacaaagaa ttaaatagag gctgcgggtt gcagactgac tttaatgtca
    25681 gccggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca gtcgaggtaa
    25741 tttttgagtg tcgtcagatt aattccaccg gtcagagcca gcgtgcagtg aggagccagc
    25801 gacggtgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc gagctgaagt
    25861 acgtctggtt gcgcgcgtaa cgcggcgatt gcctctttcg gcgtgtcggc ttcaacaact
    25921 attttcttct cgggtgcgtg gcgacgtaac tgattgattg cgcctgacca gtcctgattg
    25981 tcatgaagaa aatgacggtg gttggcaaac aataatatgg tttccgcaca tccggcacga
    26041 tgaatcagtc ctccggcagt cagaattgcc tgcgaggcca gtaagcgggt gcccggaatt
    26101 gcttttcggg tgcaggcgat attgccatca gggtaacgtt cacgaagtaa cgccagcatt
    26161 tgagtgagat aatcagaaac accgcaactc cactccagca cattctggac tgccttccat
    26221 ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgctgacc cgcgttcgct
    26281 tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa catcttacac
    26341 gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaactctat atagccatgc
    26401 tggtgtccaa tatttaatgc tcgcgtggtc aggtcaccgc cctggatatc ttccagaagt
    26461 aacgcatcga tttgtgcctg agaaaggaaa atcatcgcag tgcctcttgc ggtacgctat
    26521 cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca tcttgttgtt
    26581 gataccagcg atataaccgc tcacgctcat cgtcgttaag cgcgcccaga ctccagctga
    26641 cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt ccacggataa
    26701 aatcaacgtg agcataaatt cccatctgat acaggacgtt gagcgcaaag atatagttgg
    26761 gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg aacgaggtgc
    26821 tgaccagatg agtcgtatag acgcgaagcc gtgcctgatt attcagtttg ctcattgcct
    26881 ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag cgtggcaggt
    26941 cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtctgcc tttagcgcgg
    27001 cggcgcgacg ggcggcaacc ttcagcatcc cctgactgta atcaacgcca taaatcgtcg
    27061 tgagtttgtc agccagcgcc agcgatacgg taccaggtcc gcagcccata tcgaataagg
    27121 tctgcgcgcc ttgcagatca attttagcgg tcagttgttg caggtagctg tccgtaggcg
    27181 aggcgcaatt ttccgccatt ttttccgccc gttgatccca gtgatccggc gttttttctg
    27241 tgcggtgggc cagtttgagt tgctgcaggt aaaggtccgc aaaatcaata tcatcaatga
    27301 gcatgattgt tccttagtgg ctaattgcag agaggtgatg atgaatctgg tcggcagaaa
    27361 cccgatacag ggcagccagt ttgttcgttg ttaactgttc ggtcggtaag ccttgtgtta
    27421 ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgtttgca tggagcggat
    27481 gatgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc gtgtccagaa
    27541 gttggatctg atgaccgaaa tcgaggctgg aggcaggctc atccagtaat aacaagcgcg
    27601 gttgctggac gagtgcacgg gcgattaaag tcagctgacg ttcaccgccg ctaagcgtat
    27661 tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc gttgctttca
    27721 ggcgttcttg cttaccggga acggaaaaag cgcctatggt gggggccagg cccatcaaca
    27781 ccatatccag cacactaaaa gcaaaaatgc cgtcgtgcgc ctgcggcacc caggcaatag
    27841 cctgcgcgcg ctggctatgg ctgagtgtct gcacagggag gctatccagt aagatctcgc
    27901 cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg ccgcaaccat
    27961 tggctcctaa cagacaacaa attgttcctt gcgacagggt gaaggacacg tcgcgtagca
    28021 ccggatggcg ggagtaaccc agagacgcat gacggacggc aagcaaagtc actggcgacc
    28081 tccgcgtaac aacaaaaaca ggaaaaacgg tgcaccaaca aaagcggtca gaatgccaag
    28141 tgggatttct gttgttccaa tgctgcgtgc cagcgtatcg gtaagcagga gcagaatcgc
    28201 gccagtacac attgccatgg gcaacagctg ctgatggtta tgaccggtca acaagcgggc
    28261 aatgtgcgga acgaccaggc cgacccaacc aattatgccc gctattgcta ccgtgctggc
    28321 ggtgattagt gtggcgcaga caattaatcc aaagcgcaga cgtgtcacgt tcagtcccag
    28381 cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga gaaaaaacag
    28441 gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta aggtcacggt
    28501 ggacagcccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag gatcggccag
    28561 cgtttttatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc ctaccagcac
    28621 cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga cgcccgcgac
    28681 caccatcagc ccgccgcaaa atgcgtacag ctggatgtaa acaatggata atccccacag
    28741 gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat ccggtgacac
    28801 taagggatta cggaacattc cctgataggt ggttccggcc cctgccagcg ccgcgcccag
    28861 aaacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt gttcctgcac
    28921 aggaacattt tcttgacgca gaatgagcgc cagcagttgc tgtatatcga gatgataagc
    28981 cccacttacg gcggcaaaag tgatactgat aactgccagc agcaaaatac atccgtttac
    29041 ggttctcatt agctcgccac caatttttga tagtcggcgt cacttaatgg gcaatgccag
    29101 aacagttggg cgtaattctg catgtcactt ttaaactggc gattgatggc gggatccagc
    29161 caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc atccagccag
    29221 ccaaagggca ggccacttaa aaataagatg cgttgttccg caaccgcttt cacgccctgc
    29281 cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag aataatatcc
    29341 ggctgccagc gcaggagatt ttccatggaa acctgtgtca gaccgtggcg atcggctatt
    29401 tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga accctgtaag
    29461 cccgtttcca gccctcgagg accgcgcgca gcataaaagc tgaggttagc ggcgggtgag
    29521 gtggcgaatg cctgagcttc accaacgaag cggctggcga gattggcttg ttctgcggtg
    29581 cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga ttgttccagt
    29641 ttcccgttaa gcaataacca gggtatttgt gtctgttcgc taacctgccg tgccagggag
    29701 atccaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa cgccataagc
    29761 ccttccagcg agagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg gaactggcga
    29821 atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga aaaaccgacc
    29881 attttttctg gcgcgacagc cagcaatagt aaatcggcgg gcgcgcctgc gctgacgatg
    29941 cgtgtaatgt tttgggcttc gggaagcgtc agtgaggccc agaatttgac tgattgtgca
    30001 aaggacggaa gatgatgcag gaacaatgtc gacgcaagaa tctgtgtaaa cctccgtctg
    30061 gttagtgaca taagtaatat
//
LOCUS       DAYPHN010000043        29664 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000043 DAYPHN010000000
VERSION     DAYPHN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.043"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(65..1894)
                     /gene="glmS"
                     /locus_tag="ACG0BW_001903"
     CDS             complement(65..1894)
                     /gene="glmS"
                     /locus_tag="ACG0BW_001903"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFK7258871.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2056..3426)
                     /gene="glmU"
                     /locus_tag="ACG0BW_001904"
     CDS             complement(2056..3426)
                     /gene="glmU"
                     /locus_tag="ACG0BW_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFK7258872.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3777..4196)
                     /gene="atpC"
                     /locus_tag="ACG0BW_001905"
     CDS             complement(3777..4196)
                     /gene="atpC"
                     /locus_tag="ACG0BW_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFK7258873.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4217..5599)
                     /gene="atpD"
                     /locus_tag="ACG0BW_001906"
     CDS             complement(4217..5599)
                     /gene="atpD"
                     /locus_tag="ACG0BW_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFK7258874.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5626..6489)
                     /gene="atpG"
                     /locus_tag="ACG0BW_001907"
     CDS             complement(5626..6489)
                     /gene="atpG"
                     /locus_tag="ACG0BW_001907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFK7258875.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6540..8081)
                     /gene="atpA"
                     /locus_tag="ACG0BW_001908"
     CDS             complement(6540..8081)
                     /gene="atpA"
                     /locus_tag="ACG0BW_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFK7258876.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8094..8627)
                     /gene="atpH"
                     /locus_tag="ACG0BW_001909"
     CDS             complement(8094..8627)
                     /gene="atpH"
                     /locus_tag="ACG0BW_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFK7258877.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8642..9112)
                     /gene="atpF"
                     /locus_tag="ACG0BW_001910"
     CDS             complement(8642..9112)
                     /gene="atpF"
                     /locus_tag="ACG0BW_001910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFK7258878.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9174..9413)
                     /gene="atpE"
                     /locus_tag="ACG0BW_001911"
     CDS             complement(9174..9413)
                     /gene="atpE"
                     /locus_tag="ACG0BW_001911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFK7258879.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9460..10275)
                     /gene="atpB"
                     /locus_tag="ACG0BW_001912"
     CDS             complement(9460..10275)
                     /gene="atpB"
                     /locus_tag="ACG0BW_001912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFK7258880.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10284..10664)
                     /gene="atpI"
                     /locus_tag="ACG0BW_001913"
     CDS             complement(10284..10664)
                     /gene="atpI"
                     /locus_tag="ACG0BW_001913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFK7258881.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11281..11904)
                     /gene="rsmG"
                     /locus_tag="ACG0BW_001914"
     CDS             complement(11281..11904)
                     /gene="rsmG"
                     /locus_tag="ACG0BW_001914"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFK7258882.1"
                     /translation="MLNKLTSLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11968..13857)
                     /gene="mnmG"
                     /locus_tag="ACG0BW_001915"
     CDS             complement(11968..13857)
                     /gene="mnmG"
                     /locus_tag="ACG0BW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFK7258883.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14236..14679)
                     /gene="mioC"
                     /locus_tag="ACG0BW_001916"
     CDS             complement(14236..14679)
                     /gene="mioC"
                     /locus_tag="ACG0BW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFK7258884.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14769..15227)
                     /gene="asnC"
                     /locus_tag="ACG0BW_001917"
     CDS             complement(14769..15227)
                     /gene="asnC"
                     /locus_tag="ACG0BW_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFK7258885.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15379..16371
                     /gene="asnA"
                     /locus_tag="ACG0BW_001918"
     CDS             15379..16371
                     /gene="asnA"
                     /locus_tag="ACG0BW_001918"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFK7258886.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWEGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16376..17827)
                     /gene="viaA"
                     /locus_tag="ACG0BW_001919"
     CDS             complement(16376..17827)
                     /gene="viaA"
                     /locus_tag="ACG0BW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFK7258887.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17821..19317)
                     /gene="ravA"
                     /locus_tag="ACG0BW_001920"
     CDS             complement(17821..19317)
                     /gene="ravA"
                     /locus_tag="ACG0BW_001920"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFK7258888.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19540..21408
                     /gene="kup"
                     /locus_tag="ACG0BW_001921"
     CDS             19540..21408
                     /gene="kup"
                     /locus_tag="ACG0BW_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFK7258889.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21575..21994
                     /gene="rbsD"
                     /locus_tag="ACG0BW_001922"
     CDS             21575..21994
                     /gene="rbsD"
                     /locus_tag="ACG0BW_001922"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFK7258890.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22002..23507
                     /gene="rbsA"
                     /locus_tag="ACG0BW_001923"
     CDS             22002..23507
                     /gene="rbsA"
                     /locus_tag="ACG0BW_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFK7258891.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KAIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23512..24477
                     /gene="rbsC"
                     /locus_tag="ACG0BW_001924"
     CDS             23512..24477
                     /gene="rbsC"
                     /locus_tag="ACG0BW_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFK7258892.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24502..25392
                     /gene="rbsB"
                     /locus_tag="ACG0BW_001925"
     CDS             24502..25392
                     /gene="rbsB"
                     /locus_tag="ACG0BW_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFK7258893.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25518..26447
                     /gene="rbsK"
                     /locus_tag="ACG0BW_001926"
     CDS             25518..26447
                     /gene="rbsK"
                     /locus_tag="ACG0BW_001926"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFK7258894.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANTSALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26451..27443
                     /gene="rbsR"
                     /locus_tag="ACG0BW_001927"
     CDS             26451..27443
                     /gene="rbsR"
                     /locus_tag="ACG0BW_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFK7258895.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27409..28836)
                     /gene="mdtD"
                     /locus_tag="ACG0BW_001928"
     CDS             complement(27409..28836)
                     /gene="mdtD"
                     /locus_tag="ACG0BW_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFK7258896.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28859..29551)
                     /gene="yieP"
                     /locus_tag="ACG0BW_001929"
     CDS             complement(28859..29551)
                     /gene="yieP"
                     /locus_tag="ACG0BW_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFK7258897.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7128 a         7607 c         7591 g         7338 t
ORIGIN      
        1 attattgata tcacttataa aaaagggccg ccggtcggca gcccttttct atcaacgaga
       61 gatattactc aaccgtaacc gatttcgcca ggttacgcgg ctggtcaacg tcggtgcctt
      121 tgatcagcgc aacgtggtaa gccagcagtt gcagcggaac ggtgtagaag atcggtgcaa
      181 taacctcttc cacatgcggc atctcgatga tgtgcatgtt atcgctactt acaaaacccg
      241 catcctgatc ggcgaagaca tacaactgac cgccacgcgc gcgaacttct tcaatgttgg
      301 atttcagttt ttccagcaat tcgttgttcg gtgcaacgac gataaccggc atatcggcat
      361 caatcagcgc cagcggaccg tgtttcagtt cgcctgcagc gtaggcttca gcgtgaatgt
      421 aagagatctc tttcagcttc aatgcgcctt ccagcgcgat tgggtactga tcgccacggc
      481 ccaggaacag cgcgtgatgt ttgtcagaga aatcttctgc cagtgcttca atgcgtttgt
      541 cctgagacag catctgctca atacggctcg gcagcgcctg cagaccatgc acgatgtcat
      601 gttcaatgga ggcatccaga cctttcaggc gagacagctt cgccaccagc atcaacagca
      661 cagttaactg agtggtgaat gctttagtgg acgccacgcc aatttccgta ccggcattgg
      721 tcatcagcgc cagatcggat tcgcgcacca gagaagaacc cggaacgtta cagattgcca
      781 gcgaaccaag gtaacccagc tctttcgaca gacgcaggcc cgccagggta tccgcggttt
      841 cgccagactg tgacaaggtg atcatcaggc tgttacggcg cacggcagat ttgcgatagc
      901 ggaattcaga ggcgatttcg acgtcgcacg gaatacctgc cagcgattca aaccagtagc
      961 gggaaaccat accggagtta taagaagtac cacaggcgag gatctgaata tgctcaacct
     1021 tcgacagcag ttcgtcggcg ttcggtccca gctcgcttaa atcaacctga ccgtggctga
     1081 tgcgcccggt aagggtgttt ttgatcgcgt tcggttgttc gtagatctct ttctgcatgt
     1141 agtggcggta aataccttta tcacccgcgt catattgcag attggattcg atatcctgac
     1201 gttttacttc cgcgccagtt ttatcgaaga tgtttaccga acggcgagtg atttccgcaa
     1261 tatcgccctc ttcaaggaag ataaagcgac gagtcactgg caacagcgcc agctggtcag
     1321 aagcgataaa gttttcgccc atgcccaggc caatcaccag cgggctacca gaacgtgccg
     1381 ccagcagggt atccgggtga cgggagtcca tgatcactgt accgtacgca ccacgcagtt
     1441 gcgggatagc acgcagaaca gcctcacgca gagtaccgcc ttgtttcagc tcccagttca
     1501 ccagatgggc aatcacttcg gtgtcggttt cagaaacgaa ggtatagcca cgcgctttta
     1561 actcttcacg cagcggttca tggttttcga tgatgccgtt atgcaccacc acaatgtgtt
     1621 cagaaacatg cggatgcgcg ttagcttctg aaggttcacc gtgggtcgcc cagcgagtat
     1681 gagcaatacc ggtgccgcca tgcagaggat gttcttccgc tgcctgagcc agcatctgga
     1741 ctttaccgag gcgacgcagg cgggtcatat gaccttctgc atcaacaacg gccagaccgg
     1801 cagagtcata tccgcggtat tcaagacgac gtaaaccttc aagaaggatt tctgctacat
     1861 cacgttgcgc gatcgcgcca acaattccac acatagtttt tgattccgat ttatatcgtt
     1921 gtcggtcaac ctgtatgccc gttttttcgg gcgccccgag ccttgtagag agtggggtta
     1981 tttttatagt tactgcttgt gggcgggaga ttatgttatc tcctcatccc atatgaccgg
     2041 atttcccagc cagaattact ttttctttac cggacgacgc cagccttctt tttgagtctg
     2101 cggcacacgg ctgatcgcca gggcgttttc gccgacatta cgcgtcacag ttgtacccgc
     2161 agcaatggtc gcgcctttgc ctactgttac cggggccacc agctgagtgt cggaaccgac
     2221 aaacacatcg tcaccgataa tggtcttaaa tttattcgca ccatcgtagt tgcaggtaat
     2281 ggttcccgcg ccgatgttaa cgttatcgcc aatttccgca tcgcccaggt aagtcagatg
     2341 accagctttc gagcctttac ccagacgcgc ttttttcatc tcaacgaagt taccgacgtg
     2401 tgcaccttcc agcaactcag caccaggacg cagacgggca aacgggccaa tggtacaggc
     2461 cgccgccaga ttcgcatctt ccacaacggt atacggactg atttcgcaat catcgccaat
     2521 cacgctgttt ttaatcacgc aaccggtgcc gattttcacg cgatgaccga gagtcacgtt
     2581 gccctcgatg ataacgttag tatcaatttc aacatcgcgc ccgtgagtaa gcgtaccgcg
     2641 cagatcaaaa cgcgccggat cgcgcagcat aacgcctgct aacagcagtt tttcagcctg
     2701 ttcggactgg taaacgcgct ccagtcggga gagttgcagg cggttattca cgccttctac
     2761 ttcgcttaaa cgttgcggat gaacggcgac gatttcacgc ccttcctgat acgccagcgc
     2821 aataatgtcg gtgatgtagt attcaccctg agcgttattg ttggtcagct tcgccagcca
     2881 gcgtttcata tctgcgccgt tggcaatcag aatgccggtg ttgatctcct gaatctgacg
     2941 ctgctcgtcg gtggcgtctt tgtgctcaac aatgccggta actttgccgt tttcacgggt
     3001 gatacgtcca taaccggtcg gatcatccag ttttaccgtc agcagaccaa tgccaccctg
     3061 cggtttagca tcacgcaggc gctggagtgt ttcgacagag atcagcggca cgtcgccgta
     3121 gagcattaaa atgtcttcat catcggcaaa gaaaggtgcg gcctgctgca tcgcatgacc
     3181 tgtacccagc tgttctgcct gaagcaccca gttcaggttg tcatctttca gcgcctgttt
     3241 aagcagatcg ccgccgtgac cgtacaccag gtgaacgtgc gccgcgccta attcattcgc
     3301 agcatcaatg acatgctgaa ccatcgcttt cccggcaagg gtatgcagca ctttcggaag
     3361 atcggaatac atgcgcgtgc ctttgcctgc ggcaaggatc actacgctca tagcattatt
     3421 caacatacgc gtcctgactg taatttgaga acgaatttaa accgcttcac cttgaaaaaa
     3481 ctacattttt ttcatcgtga aatggacaga ggataaattg ttcaataacg gattatcccc
     3541 gctgacgaaa gcgccatttt cgaccatcgt cacgcctttt gtctgcagaa aattaaggca
     3601 ataccttcat ttaagcgtca ggtggatgtt tttgctctta tttcgatcaa tgaataaacg
     3661 ggaaaacagg gggttttata tcagcaggat ctatgtgaac gctcttcagg acggggcaac
     3721 gcgtaaaaaa aagccagcct gtttccagac tggcttttgt gcttttcaag ccggtgttac
     3781 atcgcttttt tggtcaactc gataacgcgc agctgcgcga tcgctttggc cagttccgca
     3841 gacgcctgag cgtaatctac gtcgccgtga gagctgctaa tgtgctcttc agccttacgt
     3901 ttcgcttcca tggctcgcgc ttcgtcgaga tcctggccgc gaattgcggt gtcggccaga
     3961 acggtcacgt tgccaggctg cacttcaaga atgccgccag acagatagat aaactcttcg
     4021 tgaccgtgct gtttcacgat gcgaatcata ccaggcttaa tggcggtgag cagcggtgcg
     4081 tggccagggt agatccccag ttcaccttcg ctacccgtta cctggatttt ctcgaccaga
     4141 ccagagaaca tttgttgctc tgcactgacg acgtccaggt ggtaagtcat tgccatatca
     4201 ccctccgatt aaggcgttaa agttttttgg ctttttccac agcttcttcg atggaaccga
     4261 ccatgtagaa cgcctgctcc ggcaggtgat cgtattcgcc ttccatgatg cctttaaagc
     4321 cacggatggt gtctttcagg gagacgtatt tacccggaga accggtgaat acttctgcca
     4381 cgaagaacgg ctgggacagg aagcgctgga tcttacgagc acgcgctacc accagtttgt
     4441 cttcttcaga cagttcatcc atacccagga tggcgatgat gtctttcagt tcctgataac
     4501 gttgcaggat ggactgaacg ccacgcgcgg tgtcgtagtg ttcctgacca accaccagcg
     4561 ggtccagctg acggctggtg gagtccagcg ggtcaacggc cgggtagata cccagagacg
     4621 cgatctgacg gctcagtacc acggttgcgt caaggtgcgc aaaggtggtt gccggagacg
     4681 ggtcagtcaa gtcatccgca ggtacgtata ccgcctgtac ggaggtgata gaaccagttt
     4741 tggtggaggt gatacgttcc tgcagaacgc ccatctcttc cgccagggtc ggctgataac
     4801 ctaccgctga aggcatacgg cccagcagtg cggatacttc cgtaccggcc agggtgtaac
     4861 gatagatgtt gtcaacgaac agcagaacgt cacgaccttc gtcacggaat ttctcagcca
     4921 tggtcagacc ggtcagagca acgcgcagac ggtttcccgg cggctcgttc atctggccat
     4981 acaccaggga tactttgtcg ataacgttgg agtcggtcat ttcgtggtag aagtcgttac
     5041 cctcacgagt acgttcacct acgcccgcaa acacagagta accggagtgc tcgatcgcga
     5101 tgttacgaat aagctccatc atgtttacgg ttttacctac acccgcacca ccaaacagac
     5161 caactttacc gcccttagcg aacggacaca tcaggtcgat aactttgata ccggtttcca
     5221 gcagttcctg agagtttgac agctcttcgt aggaaggtgc tgcgcggtga atcgcccaac
     5281 gctcttcttc accgatctcg cctttcatgt cgaccggttc gcccagtacg ttcatgatac
     5341 ggcccagagt cgctttacct accgggactt cgatcgggtg ttcgaggtct tttacatcca
     5401 gaccgcgacg cagaccgtcg gaggaaccca ttgcgatggt acgtacgata ccgccgccga
     5461 gctgctgctg aacttccagc accagacgct cattaccatt ttgcacctca agagcatcgt
     5521 acacgcgcgg tacggcatcc tgagggaatt cgacgtcaac tacggcgccg attacctgga
     5581 caatctttcc agtagccatc ttaaatcctc tacgaaataa cctgtttaaa ccgcggcggc
     5641 ccccgagacg atctcggtga gttcctgagt aatgctggcc tgacgagctt tgttgtatac
     5701 caactgcagc tctttaatca ggctgccgcc attgtcggtc gcggctttca tcgccaccat
     5761 acgggcggcc tgctcgctgg ccaggttttc aaccacgccc tgataaacct gagattcgac
     5821 ataacgacgc agcagggtat ccagcaacgc cttcggatcg ggttcgtaca ggtaatccca
     5881 ggatttatgt ttcagatcat catcatctga tgccggtaac ggcagcagct ggctgatggt
     5941 cggaacctga gacatggtgt taataaattt gttgctgaca atgtaaagct tgtccagacg
     6001 gccttcgtcg taggcctgca acatcacttt taccggaccg atcagttcgg acagggaagg
     6061 gttatccccc atgccggtga cctgggcaac aacattgccg cccacggagt tgaagaacga
     6121 cacgcctttc gagccgatca ttgcgaggtc gcattgaacg cctttgtcgg tccaggtctt
     6181 catttccgcc agcagttttt tgaacaggtt aatgttcaaa ccaccgcaca aaccacggtc
     6241 ggtcgacacc accaggtagc ccacgcgttt aacgtcgcgg tcttccaggt aagggtgctt
     6301 atattccaga ttaccgtgtg caaggtgacc aatcactttg cgcatggttt ctgcataagg
     6361 acggctggcc gccatgcgat cctgcgattt acgcattttg gaagcggcga ccatctccat
     6421 cgctttagtg atcttttgcg tgttctggac gcttgcgatc ttactacgta tctcttttgc
     6481 gccggccatg agcttctcct caatgccttg cggcctgccc taaggcaagc cgccagacgt
     6541 taccaggatt gggttgcttt gaaggaatcg aggatgcctt tcagcttgcc ttcgatttcg
     6601 tcgttgtagc caccggtctg gttgatctct tgcatcaacg gagcgtgatc acggtcgacg
     6661 taagccagca gagcggcttc gaagctgccg attttcgaca gttcaacatc cgccaggtaa
     6721 ccacgttctg ctgcgaacag aaccagagac tgttgcgcta cggacatcgg cgcatactgt
     6781 ttctgtttca gcagttcggt cactttctga ccgtggtcaa gctgcttacg tgttgcatcg
     6841 tcaaggtcgg atgcaaactg agagaacgct gccagttcac gatactgtgc cagagcggta
     6901 cggataccac cggacagttt tttcatgatc ttggtctgtg ctgcaccacc aacacgggat
     6961 acggaaatac ccgggttaac cgcaggacga ataccggcgt tgaacaggtt ggtttccagg
     7021 aagatctgac catcggtaat ggagattacg ttggttggaa cgaacgcaga aacgtcaccc
     7081 gcctgagttt cgataatcgg cagcgcagtc agagaaccgg ttttcccttt cacttcacct
     7141 ttggtgaagg cttcaacgta ttcggcgtta acacgcgcag cacgctccag cagacgagag
     7201 tggaggtaga atacgtcgcc cgggaatgct tcacgtcctg gcggacgacg gagcagcagg
     7261 gagatctgac ggtaagcaac agcctgttta gacaggtcat cgtaaatgat cagcgcatct
     7321 tcaccgcggt cacggaagta ttcgcccatt gcgcaaccag cgtacggtgc caggtattgc
     7381 agtgcagcgg attcagacgc ggttgctacc acaacgatgg tgttagccag tgcgccgtgc
     7441 tcttccagtt tacgtaccac gttagaaatg gtggacgctt tctggccgat agcgacatag
     7501 atacatttga taccggaatc gcgctggttg atgatggcat cgatagccag tgcggtttta
     7561 ccggtctgac ggtcaccgat gatcaattca cgctgaccac gaccgattgg gatcatggag
     7621 tcaacggctt tataaccggt ctgtaccggc tgatctacgg actgacgttc gataacgccc
     7681 ggagcgattg cttctacagc agagaagccg tcgtgatcca gcggaccttt accgtcgatt
     7741 ggtgcaccca gagtgttaac cacacggccc agcaggccac ggccaaccgg aacttccagg
     7801 atacggccag tacacttaac tttcatgcct tcggcaaggt cagcgtacgg acccataaca
     7861 accgcaccta cagagtcgcg ctcgaggttc agtgcgatag cgtaacggtt acccggcagg
     7921 gagatcattt caccctgcat acaatcggcc aggccgtgaa tgcggataac accgtcactt
     7981 acagaaacaa tagtaccttc gttgtgagct tcactcacaa cattgaactg agcaatgcgc
     8041 tgcttgatca gttcgctgat ttcggtggaa ttcagttgca tgctccagtc cccttaagac
     8101 tgcaagacgt ctgcaaggcg ctcaagacga ccgcgtacgc tgccatcaat gaccatatca
     8161 cccgctcgga tgataacgcc tgccattaca gacttatcga ttttgcaatt cagcttaact
     8221 ttgcgtgaca gacgtttttc catcgcagca gaaattttcg cgagctgttg ttcactcagt
     8281 gcggcagcgg aaatgacgtc tacctcagcg gtagcctcac tcacggcacg caggtgaata
     8341 aactgctcca gaacatccgg gagcgcgtta agacgaccat tttcagccat tacccgaatc
     8401 aggttctgac cgttttcgtc cagttgctca ccacaaactg cgataaacga ctcggcgagc
     8461 gtttctggcg caagcgcgcc agagagaagc tctgccattt gttcgttttt ggttacctcg
     8521 gcggcaaacg ccagcatgtc ctgccagcgt tctacacttt ggtgttcgac ggcaaagtca
     8581 aaagctgctt tggcgtaggg gcgagctacc gtaataaatt cagacatcag cccctccctc
     8641 cttacagttc agcgacaagt ttatccacga tgtcgctgtt agcagcttca tccacggaac
     8701 gttcgatgat cttctcggcg ccagcaacag ccaggatagc aacttgctta cgcagctctt
     8761 cacgggcacg tttacgctcg gcttcaattt ccgcctgcgc ctgggccacg attttagtac
     8821 gttcctgttc tgcctcagct ttcgcttcgt ccagaatctg cgagcggcgt ttgttagcct
     8881 gctcgatgat tacctgggct tccgctttcg cttttttcag ctggtcggtc gcgctggcct
     8941 ttgcaaggtc aaggtcctta tgtgctcgtt ctgcggaagc aagaccgtca gcaatttctt
     9001 tttgacgttt ttcgatggct gccattaatg gcggccatac gtacttcatg cagaacagaa
     9061 cgaacaggac aaacgcgatg gcctggccga ggattgttgc gttaagattc acagcacaat
     9121 gcctctattt agttaacgtt ctgatattgc tctttaaata aaagcaacgc ttactacgcg
     9181 acagcgaaca tcacgtacag acccagacct acagcgatca tcgggatagc atccaccaga
     9241 cccataacga taaagaactg agtacgcagc agaggaatca gatcaggttg acgcgctgcg
     9301 ccttccagga atttaccccc gaggatgccg ataccgatcg cagcaccgat tgccgccaga
     9361 cccatcatca cagcggcagc catgtacagc agatccatat tcaggttttc catgacagtc
     9421 tccagtttgt ttcagttaaa acgtagtagt gttggtaaat taatgttctt cagacgccat
     9481 cgacagatag acgatcgtca gaaccatgaa gatgaaggct tgcagcgtaa tgatcaggat
     9541 gtggaaaatg gcccacggca cattcagaat ccactgtgac caccacggca acagaccagc
     9601 aatcagaatg aaaatcagct caccggcata catgttaccg aacagtcgca aaccgagtga
     9661 aactggtttg gacagcaggc ttaccccttc aaggattaag ttgacaggaa tgaacgccca
     9721 gtgattgaac ggctgcagcg tcaactcttt cgtgaagccg ccgatgcctt tcattttgat
     9781 gctgtagaac agaatcagga taaatacacc cagtgccata gacagcgtta cgttcacgtc
     9841 cgcagacgga accacacgca gtgcaggcag acccagtaca tgttcagcaa tgtacggcag
     9901 caggtcgata ggcagtaaat ccatcaggtt catcaggaat acccagacga agatcgtcag
     9961 ggccagcgga gcaatcagct tgcttttgcc atggtacatg tctttcacgc taccattaac
    10021 aaagccgatc accagctcaa tcgcggtctg aaacttacct ggcacaccgc tggtcgcctt
    10081 tttggctacg ctacggaata aaaccaggaa caacagaccc agcaccaccg agaagaacat
    10141 ggagtcaata ttgattgtcc agaaggtggc tggggggtta tgtggatcca ccagcgagaa
    10201 tgtacgcagg tccagctgaa ggttgttcag gtggtgtcct atgtaatcct gcggcgtcat
    10261 attttctgaa gccatgatgc cttttaccct ttgttgttaa ttacagccgg tgccagtatc
    10321 tgaaccacca gcaccaaaac ccacgtaacg atcagcggca agaataccgc ctttaaaacc
    10381 gccaacgcca ccacaagtaa caccaacatc gccagaactt tgaaagcttc gccaaatgcg
    10441 aatgtccagg ccacacggcc tttcgctggt gtatgcgcct ggtgacgcca ggcaaatatc
    10501 ataaacaaaa cgttaggcag aaagactgcc aggcccccgc ttattgcaga gacgccccag
    10561 aaggggtctt tgaggctgaa cagcaatcca cttgctatca ccaccagtaa ctgaacgagc
    10621 agaagcttcc gagcaacgtt tcgactcacg agcgacacag acatcacgtt tttcactcct
    10681 gctcccttcg aggtatgccg cgtgtcgtat aaaactttct ttaaggctta gagtcaagca
    10741 tcaaaaagcg gtcaaattat acggtgcgcc cccgtgattt caaacaataa gtagccaaaa
    10801 ggtgaataaa tgtttaaata tttttccagt gcatacaatt gcgacttttc tgcaaaccct
    10861 gttcgatcat gaaaaactgt aaataacgcg taaacactgg tgataaagcg tgcttcagat
    10921 cacatattga gcatgttcgc gcacagcata tttatttact tggcaaatga tgcctttgca
    10981 agtttatgat atttcagtct aaaaacagat actgttttaa taaatgacat ttacacaaca
    11041 aaaaccaccc attgacattt ttaataatgt tttaacagcc aatgatgatt cttagcgccg
    11101 atttttagca gactgatatt ttcacttatg acttattttc tgcttaccaa aaaaagccac
    11161 gttactttgt tgatgcaaaa gagtgaacgt ggcgttaaat gtaaccagtt atatcagtag
    11221 aaaacctggt tgttgttaac agtctaaccg gtcaattttt tatgattttt ttgataaaaa
    11281 ttaaatttta tttgctttaa tcaccaccag atgacgttcg ccatccaggg ctggaacctg
    11341 aagtttaacc actgattcga cctgatattc ttcgggcaac aaagcgattt catcttccgg
    11401 catttgccct ttcagcgcgt agaaacggcc ttgctcacca ggaagatggt ggcaccagct
    11461 caccatatcg ttcagagagg caaaagcgcg gctaattacg ccatcaaatg gcggctcaga
    11521 tggaaactct tctaccctgc tctgtactgg ctcaatattc tccagtttaa gctcatgttg
    11581 cacctgacga aggaaacgca cgcgtttacc aaggctatcc aacaacgtga agtgggcttc
    11641 aggacgcacg atagagagtg gaatgccagg cagtcctggt ccggtgccga catcgataaa
    11701 ccgttcacct tgcagatacg gtgccaccac aatgctatcg agaatatggc gtaccagcat
    11761 ctcattagga tcgcggaccg aagtcaggtt gtacgcttta ttccatttat gcagcatatt
    11821 cacgtaggca attagctggt ttttctggtg atcggtaagc gaaattcctg cgtctttcag
    11881 cagagaggtg agtttgttta gcacggtgat tacctgttct tgatgcgttg cccggtaagc
    11941 gggtgcttac caggcatttt taatgcgtta tgcgctacga cgcagcatac cctgtttttt
    12001 cagccacacc agcagaatgg agatggccgc aggcgtgacg ccagaaatac gcgaagcctg
    12061 accgatagag gccggtttgt gatcgttaag tttggcgatc acttcgttag aaagaccgga
    12121 tacctggcgg taatccagcg tcgctggtag cagggtgttc tcgttacgca gctgcttttc
    12181 gatctcatct tgctggcgcg cgatataacc ttcgtattta acctgaatct caacctgttc
    12241 cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc agcgtggtta atttttcata
    12301 agtcatttcc ggacgacgca gcagatcttc accactggct tcacgggaaa gtggcgcagt
    12361 caggtgagca ttcacttcgg ctgcagcttc cgccgacggg gttacccagg tcgatttcag
    12421 acgctgacgc tcacgctcga tattctcaag tttctcgtta aagcgcgccc aacgttcgtc
    12481 atccaccagg cccagttcac gaccgatttc agtcaaacgc agatccgcat tatcttcgcg
    12541 cagcatcaga cgatattctg cgcgcgaagt aaacatacga tacggttctt tggttcctaa
    12601 agtgcacagg tcatcaacca gtacgccgag atacgcctga gaacgcgccg gagcccaacc
    12661 ttctttgtca gcagacagac gggcagcgtt aagaccagcc agcaaacctt gcgcagcggc
    12721 ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag aacagcccct ggataaactt
    12781 gctctccagc gtcggtttca ggtcgcgtgg atcgaagaag tcatactcaa tggcataacc
    12841 cggacgcacg atcttcgcgt tttccatccc ctgcatggag cggacgattt gcatctgcac
    12901 atcgaacggc aggctggtgg agataccgtt cggataaatt tcgtttgagg tcagcccttc
    12961 cggttcaagg aagatctgat gctgatttct gtcggcgaag cgcatgactt tgtcttcgat
    13021 cgacgggcag tagcgtgggc cgacaccttc gatcacccct gcatacattg ggctacgatc
    13081 gaggttactg cggatcacat catgggtttt ctcgttggta tgggtgatat aacacggcac
    13141 ctgctgtgga tgctgggacg cattacccat aaacgagaat accggcattg ggttatcgcc
    13201 atgctgttgc gccagcacgc tgaagtcgat agtacgcgca tcaatacgcg gcggtgtccc
    13261 ggttttcaga cgaccaacgc gcagcggcag ttcacgcaaa cggcgagaga gcggaatgga
    13321 cggcggatca ccagcacgac caccgctgta gttatccaga ccgatatgaa ttttaccgtc
    13381 gaggaacgtc ccaacggtga gcacgacggc tttggcacgg aacttcagtc ccatttgggt
    13441 aacagcacca accacgcgat cgttttcgac aataagatct tcaaccgcct gctggaagat
    13501 catcaggttc ggttggttct ccagcgccgt acgtaccgcc tgccggtaga gcacacgatc
    13561 cgcctgagca cgggtagcgc gaaccgctgg tcccttgctt gcgtttagta tcctaaactg
    13621 gatacccgcc tgatcgatcg ctttcgccat cagaccgccg agtgcatcca cttcttttac
    13681 cagatgtccc ttcccaatac cgccgatcgc cgggttgcag ctcatctgcc ccagagtgtc
    13741 gatattgtgt gtcaaaagca gagtctgttg acccatacgc gccgcggcca tcgcggcctc
    13801 ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa ggatccggat aaaacatggt
    13861 gattgcctcg cataacgcgg tatgaaaatg gattgaagcc cgggccgtgg attctactca
    13921 actttgtcgg cttgagaaag acctgggatc ctgggtatta aaaagaagat ctatttattt
    13981 agagatctgt tctattgtga tctcttatta ggatcgcact accctgtgga taacaaggat
    14041 ccagctttta agatcaacaa cctggaaagg atcattaact gtgaatgatc ggtgatcctg
    14101 gaccgtataa gctgggatca gaatgagggg ttatacacaa ctcaaaaact gaacaacggt
    14161 tgttctttgg ataactaccg gttgatccaa gcttcctgac agagttatcc acagtggatc
    14221 gcacgatctg tatacttatt tgagtaaatt aatccacgat cccagccatt cttctgccgg
    14281 atcttccgga atgtcgtgat caagaatgtt gatcttcagt gtttcgcctg tctgttttgc
    14341 accggaattt ttgagttctg cctcgagttt atcgatagcc ccacaaaagg tgtcatattc
    14401 acgactacca ataccgattg cgccaaagcg gaccgcagaa agatcgggct tctgttcctg
    14461 caatgcttca tagaaaggag aaaggttgtc cggaatatct ccggcaccgt gggttgagct
    14521 gataaccagc cagatccccg aggcagataa atcttccaac agcggaccgt gcagcgtttc
    14581 ggtggtaaaa cccgcctctt ccagcttttc agccaggtgt tctgccacat attcggcacc
    14641 gccgagggtg ctgccgctga taagagtgat atctgccata aaccgccacc tttattaaga
    14701 gtggcgtatt gtacgctgtg aacgcgttgg gatctacctg tggaaaagta tgggattaaa
    14761 aaagccgatc agggcttaat ggtacgcatg atcgggttct gcaggacgat cagtgtctcg
    14821 gtggactgaa tttcatcaat tgtttggatc ttgttgataa gtacatgctg gagagcatcg
    14881 atcgaacggc acatcacttt gataaagatg ctgtagtggc cagttgtgta ataggcttca
    14941 gtgacttcat cgaggctttc cagctttgcc agcgcggaag gatagtcttt ggcgctcttt
    15001 aatataatgc cgataaagca gcctacgtca taaccgagct gcttcgggct gacatcaata
    15061 cgcgccccgg taatgatccc cgcctgcttc attttctcta ctcgaacgtg aattgtcccc
    15121 ggactgacgc caaattgttt cgccagttcg gcgtaagcgg tgcgcgcatt gcccattaat
    15181 gcttccagga tgccacggtc cagattgtcg atcagataat tttccatagg attttcttat
    15241 gaggattgat gattcattct attttagccc tattttttaa tgaatcaaaa gttcatgagg
    15301 ctttttattg aatgattatt gcatgcgtgt cggtttttgt tgcttaatca taagcaacag
    15361 gacgcaggag tataaaaaat gaaaaccgct tacattgcca aacaacgtca aattagcttc
    15421 gtgaaatctc acttttctcg tcaactggaa gaacgtctgg ggctgatcga agtccaggcg
    15481 ccgattctta gccgtgtggg ggatggcacg caggataact tgtcgggctg tgaaaaagcg
    15541 gtgcaggtaa aagtgaaagc tctgcctgat gcccagttcg aagtggttca ttcactggcg
    15601 aagtggaaac gtcagacctt agggcaacac gacttcagcg cgggcgaagg gctgtacacg
    15661 cacatgaaag cccttcgccc cgatgaagac cgtctttccc cgttgcactc ggtctatgtt
    15721 gaccagtggg attgggaacg cgtaatgggc gacggcgagc gtcaattctc gactctgaaa
    15781 agcacggtag aggcgatctg ggagggaatt aaagcaaccg aagctgcggt tagcgaagag
    15841 tttggcctgg caccgttcct gccggatcag atccacttcg tacacagcca ggagttactg
    15901 tctcgttatc cggatctcga tgccaaaggg cgtgagcggg cgattgcgaa agatcttggc
    15961 gcggtattcc tcgtcgggat tggcggcaag ttgagtgatg gtcatcgtca cgacgtgcgc
    16021 gcaccggatt atgatgactg gagcaccccg tcagagctgg gccatgcggg tctgaacggc
    16081 gatattctgg tatggaaccc ggtactggaa gatgcgtttg agctttcttc catggggatc
    16141 cgcgtggatg ccgatacgtt gaagcatcag ctggcgctga ccggtgatga agatcgtctg
    16201 cagctggagt ggcatcaggc gctactgcgc ggtgaaatgc cgcagaccat cggcggcggt
    16261 atcgggcagt ctcgtttgac catgctgctg ctgcaactgc cacatatcgg ccaggttcag
    16321 tgtggtgtat ggccggcagc tgttcgcgag agcgtccctt ctctgctgta ataatttatc
    16381 gccgccagcg tctgagcagg cggcttcgca tcccggtatc aaagcgccag atatgatcga
    16441 aaatgcgcat gatgccgggt ttgccatgtg ccgacatcgc cacggcatga aagcgatgct
    16501 gatgtacccg ctgtagctct ttcactttac tcgtcacgtc gtcaggcaac cgctgggcga
    16561 taaaatcaga aatcaccacc gcatcggcat caaaccattc cctgctttgc aagcgttcca
    16621 taatggcgcg aaaacaactg gcaagatcgg tgccgccacg aaactgctgg cttaaaaaac
    16681 ggattgcttg ttcgatgcct tgtgggcctg aaagctcata acggacgatc tcggtggaaa
    16741 atagcataat atagcagcgc cggttttcag cgagagcaat gcgcatcaag gccaggcaga
    16801 acgctttcgc acattgttca ttaaagccgc ccattgagcc ggaagtatcc acgcagacaa
    16861 taaacggtcc gcgcggctgt tcgtcgtaat ctttatgcac caccgggcgt tcgatcactt
    16921 tttcacgcca cgactcaccg tgcaggcgat aggtgagcaa ctgtttttcc accagccgac
    16981 ggtaaaactc atactccagt tccgttatcc ccagtgtcgc cagttctggc ggcaggagac
    17041 gtaaaatatc atcgctttgt tgcagaccat caacctgctc aggaaccgtc gccggttcgc
    17101 gcaccagggt gcggaaggtt tccatctgcg catcgttgcg cggtattgat ttggcttccc
    17161 gggaacgccc cagctgttct gccaggcgtt tcagttccgg ctgttcgtta agaaattcac
    17221 cgtatttcac aatcaactga tagtcgccac gtttaagctg accggcgctc atatcccaca
    17281 gacgaccagc tgcggtattg ttatctgcga gaatcggttc aagttgtccg ctcagcgtca
    17341 tgcgttcctg aacttcactc aacagttgtt cgcgttcttc ttctaatagc tgttgattta
    17401 acgtcgttgc ttgcacgatc agacttaaac gccaacgctg gagaaaaagc gtgtgtaacg
    17461 ctgaagtgat cgtgctgtta gcatcaacca actgtcgggc ttgttctgcc catggggaat
    17521 tcagacgatg cagtaagtcc aggatctgtg gtagctgcac aataaactgc ggcgtggaga
    17581 ggagctggct ttgctgatag cacatcacct cttcggtgag ttccggcggg actcgggcat
    17641 ctttcagccg actgcgcagc gcctcacgcc agcggggaac atcatcagtg attgccgcct
    17701 tcagtcgtgg gaatttttca aagaagactg ccagctgcgg tgaggccagc agcgcgatga
    17761 tcatctcttc gatcaatccc tcttcgctga cggccagcat cacattaagc gtatccagcg
    17821 ttagcattgt tgtgcctggc gaatctgtgc gccgacatcc tgtaggctgg cttcaatgcg
    17881 acctaaccag tcgccaggga taaacagaca tttttgctgt tcgctgaata aagcgtgttg
    17941 cttgcgccag tcgctttcaa gctcttccag ttgttgtttt atttcacctg gcagaccttc
    18001 agccgatgaa ccgggaagtg ccagcgtact gccttgtaaa cttacatcgc gtacaacaag
    18061 atgttgggcg ctatccactt ccagattcag tttctgggca aagccgatac cgttcagttt
    18121 gccgcgaatc tcaccacctt tactcagcca ctgttccagc gcgctacgct caaaggagat
    18181 atgaaccact tccatatcat gcaattttaa cggtttttgc agcagcagag tcagagtgga
    18241 agcagtaacg ttaacaggaa gttgatactg ctgacgacgg ctgaaaatgc cgcccagacg
    18301 aattaccgtt aaggctgttt tatcgctttg ttgctgctgg agttgcaggt gacgttgcac
    18361 aatcgcgccc aggcgggtca acatcccttg ctgttgccag gcatgaccgg tcatcaatac
    18421 atcaatttgt tgttgtatca aattcaggct ttgcgcgtca taccacaggc aatctttcag
    18481 caaaatgaga tcaaccgggg caacagcact gcgaccgcta aaaaaggcgc tggcctgcaa
    18541 taatcgaatc gcttttttcc agcgacgatc cgagacataa ggcgcatccg gtaatttatc
    18601 cagttgctgg cgcagcataa aaatcagctc aaatacatga tcgggcagcg taatttcacc
    18661 aatctctttc tgccagcgtt catattcttc atctgtgacc tgcaaggagg caggaaccgg
    18721 attgtcgttt tcatcctgtt gactggtcag catggagcgg aaattcgctt tatcctgcac
    18781 tttatctaac cacagacgaa tcagcatgcg gtcatataac gcttccagac tgctgtctgc
    18841 ttccggcagc tcgttggagg ccgccaccag cagacgcatc gggatttttt ctacgtgtgc
    18901 accgttgcgg aactggcgct cgttaatggc ggtgagcagg gtattaagaa ttgccggacc
    18961 cgctttccag atctcatcca gaaagacgat ttctgcttcc ggcaggtaac cgctggttaa
    19021 acgttcatag cgcccttcat cttttagcgc ctgaatagaa aggggaccaa aaacctcttc
    19081 cggcgtggag aagcgagtca tcagatattc aaacgcgcgg gcattctgaa aggcgaattt
    19141 taagcgccgg gcgatcaaac ttttggcaat acctggcggg ccaaggagga acacactttc
    19201 accacttaat gccgctaata aacacaagcg gatggcgtgg ctacgttcat aaagcccctt
    19261 ttccaacgaa ctgctcaggc gggaaattct ttccgctaat aaatgagggt gagccataat
    19321 gaagtggcgt cctttcgtca aaagttctgc gtaaattgcg agtatagacg tttcttgctg
    19381 gtggctaaaa tagtctcaaa gggggggtat ttttctttga gccaggttaa tgtggccgca
    19441 tttaggagta cgattttgcc gttaatcgtg catactgtgc gcttttttgt gggccaaggg
    19501 actaagcaca catttcatat ttcaacgaaa gactagtcta tgagcactga taataagcaa
    19561 tcattgcccg cgattaccct cgcggcgatt ggagttgtct acggcgatat tggtaccagc
    19621 ccgttatata cacttcgtga atgtttgtcc ggccagtttg gttttggcgt tgaacgcgat
    19681 gccgtgtttg gctttttatc gctgatcttc tggctgctaa tctttgtggt ttccattaaa
    19741 tatctcacct tcgtgatgcg ggcagataac gccggtgaag gggggatcct gacgttgatg
    19801 tcgcttgctg ggcgtaatac gtcggcgcga accacatcaa tgctggtgat tatggggcta
    19861 atcggcggca gctttttcta tggtgaagtc gtcataacac ccgctatttc ggtgatgtca
    19921 gccattgaag gtctggaaat cgtcgccccg cagctggata cctggatagt tcccctctca
    19981 attatcgttc tcactttatt atttatgatt caaaaacatg gcacggctat ggtcggtaag
    20041 ctgtttgcgc cgatcatgct gacctggttt ttgattctgg caggactggg gttacgtagc
    20101 attattgcta acccggaagt gctgcatgcg ctgaatccga tgtgggcggt gcatttcttc
    20161 cttgaataca aaacggtttc ttttattgca ttaggggcag tggtgctgtc gattacgggg
    20221 gtcgaggctc tgtatgctga tatggggcac tttggtaagt tccctattcg cctggcgtgg
    20281 ttcaccgtcg tattaccttc cttaactctt aattacttcg gtcagggcgc tctgttgtta
    20341 aagaacccgg aagcaattaa gaacccgttc ttcctgttgg caccggactg ggcgctgatc
    20401 ccgctgctga tcatcgccgc actggcgacg gtaattgcct cgcaggcggt tatctctggc
    20461 gtcttctcat tgacgcgtca ggcggtacgt ctgggatatt tgtcgccgat gcgcattatt
    20521 cacacctccg aaatggagtc agggcaaatc tatattccgt ttgtgaactg gatgctctat
    20581 gtcgcggtcg tgattgtgat tgtcagcttt gagcactcca gcaacctggc ggcggcgtac
    20641 gggattgcgg tgaccggaac catggtgctg acgtctattc tctcgactac cgtggcacgt
    20701 cagaactggc actggaataa gtattttgtt gcgctgatcc tgattgcttt cctttgtgtc
    20761 gatattccat tgttcaccgc taacctcgat aaactgctct ccggcggctg gttgccattg
    20821 agcctcggta cggtgatgtt tatcgtgatg accacctgga agagcgagcg tttccgcttg
    20881 ctgcggcgga tgcatgaaca tggcaactct ctggaggcga tgattgcttc gctggagaaa
    20941 tcaccgcccg ttcgcgtgcc tgggaccgcg gtgtatatgt cacgtgcaat caacgtcatt
    21001 ccctttgcgc tgatgcataa ccttaaacat aacaaggtat tgcatgagcg ggtgattctg
    21061 ttaactctgc gcaccgaaga tgcgccatat gtccataacg tccgtcgggt acagattgaa
    21121 caactgtcgc ccacgttctg gcgcgtggtg gcaagttatg gttggcgaga aacgccaaac
    21181 gtggaagaag tattccaccg ctgcggtctg gaaggattaa gttgccggat gatggaaacc
    21241 tccttcttta tgtcgcatga gtcgttgatc ctcggcaaac gcccgtggta tttgcgtctg
    21301 cgcggtaagc tgtacttgct gttgcaacgt aatgcgctgc gtgcgccgga tcaatttgaa
    21361 atcccgccaa acagggttat cgaactgggt actcaggtcg aaatctaacg ccagacgcct
    21421 cctttctcca taagggggcg tttttgtttt catggttaat caccatgtaa aacgtttcga
    21481 ggttgatcac atttccgtaa cgtcacgatg gttttcccaa ctcagtcagg attaaactgc
    21541 gggtcagcga aacgtttcgc tgatggagaa aaaaatgaaa aaaggcaccg ttcttaattc
    21601 tgatatttca tcggtgatct cccgtctggg acataccgat acgctggtgg tgtgtgatgc
    21661 tggtttaccc atccccaaaa gtacaacgcg tatcgatatg gcattaaccc agggtgtacc
    21721 ttcttttatg caggtgctgg gcgtcgtcac aaatgaaatg caggtcgagg cagccattat
    21781 cgcggaagag atcaaacaac ataatccgca actccacgaa acgttgctca ctcaccttga
    21841 gcagctgcaa aaacaccagg gaaataccat tgaaattcgt tacaccacgc atgaacaatt
    21901 caaacaacaa accgcagaaa gtcaggcggt aattcgcagc ggagaatgtt ctccgtatgc
    21961 gaatatcatt ctctgtgctg gcgtgacgtt ctgaggccgt catggaagca ttacttcagc
    22021 ttaaaggcat cgataaagcc ttcccgggcg taaaagccct ctcgggtgca gcgttaaatg
    22081 tctatcctgg ccgcgtgatg gcgctggtgg gcgaaaacgg tgcgggtaaa tccaccatga
    22141 tgaaagtgct tacgggtatc tatactcgcg atgccggtac gcttttatgg ctggggaaag
    22201 aaacgacgtt taccgggccg aaatcttccc aggaagccgg gattgggatt atccatcagg
    22261 aactgaacct gatcccgcag ttgaccattg ccgaaaacat tttcctcggt cgtgagtttg
    22321 ttaatcgctt tggcaaaatt gactggaaaa ccatgtatgc cgaagcggat aaattgctgg
    22381 ctaaacttaa cctgcgcttt aaaagcgaca agctggtggg cgatctttcc atcggtgacc
    22441 agcaaatggt tgaaattgcc aaagtgctga gctttgagtc gaaagccatc attatggatg
    22501 aaccgaccga tgcgctgacc gataccgaaa ccgaatcctt gttccgcgtc atccgcgagc
    22561 tgaaatcgca aggccgcggt attgtctata tttcccaccg catgaaagaa atcttcgaga
    22621 tttgcgatga cgttaccgtt tttcgtgatg ggcaatttat tgctgagcgt gaagtagcat
    22681 cactgaccga agattcgctg attgagatga tggtgggccg caagctggaa gatcaatatc
    22741 cgcacctgga caaagcgcca ggagatattc gcctgaaagt cgataatctc tgcggacctg
    22801 gcgttaacga tgtctctttt actttacgca aaggcgaaat tcttggcgtc tctggtttga
    22861 tgggcgcagg tcgtaccgaa ctgatgaaag tgctctacgg cgcactgccg cgcaccagcg
    22921 gttacgtcac cctggatggg catgaagttg ttacccgttc accgcaggat ggcctggcaa
    22981 acggcattgt gtatatctcc gaagaccgta aacgtgacgg tttagtgctg ggcatgtcag
    23041 taaaagagaa tatgtcgctg accgcgctgc gctacttcag ccgcgctggc ggcagtttga
    23101 agcatgccga tgaacagcag gcagtaagtg atttcattcg tctgtttaat gtgaaaacgc
    23161 cgtcgatgga acaggcaatt ggtctgcttt ccggtggcaa tcagcaaaaa gtggcgattg
    23221 cccgtggtct gatgacacgc cccaaagtgt tgatcctcga tgagcctacc cgtggcgtag
    23281 atgtcggcgc gaaaaaagag atctatcaac tgattaacca gttcaaagcc gatggcttga
    23341 gcatcattct ggtgtcatcg gagatgccag aagtattagg catgagcgat cgcatcatcg
    23401 tcatgcatga agggcatctc agcggggaat ttactcgtga gcaggccacc caggaagtgt
    23461 taatggctgc cgctgtgggc aagcttaatc gcgtgaatca ggagtaaaaa aatgacaacc
    23521 cagactgtct ctggtcgccg ttatttcacg aaagcgtggc tgatggagca gaaatcgctt
    23581 atcgctctgc tggtgctgat cgcgattgtc tcgacgttaa gtccgaactt tttcaccatc
    23641 aataacttat tcaatattct ccagcaaacc tcggtgaacg ccattatggc agtcgggatg
    23701 acgctggtga tcctgacgtc gggcatcgac ttgtcggtag gttctctgtt ggcgctgacc
    23761 ggcgcagttg ctgcatctat cgttggcatt gaagtcaatg cgctggtggc tgttgctgct
    23821 gctctcgcgt taggtgccgc aattggtgcg gtaaccgggg tgattgtagc gaaaggtcgc
    23881 gtccaggcgt ttatcgctac gctggttatg atgcttttac tgcgcggcgt gaccatggtt
    23941 tataccaacg gtagcccagt gaataccggc tttactgaga acgccgatct gtttggctgg
    24001 tttggtattg gtcgtccgct gggcgtaccg acgccagtct ggatcatggg gattgtgttc
    24061 ctcgcggcct ggtacatgct gcatcacacg cgtctggggc gttacatcta cgcgctgggc
    24121 ggcaacgaag cggcaacgcg cctttctggt atcaacgtca ataaaatcaa aatcatcgtc
    24181 tattctcttt gtggtctgct ggcatcgctg gccgggatca ttgaagtggc gcgtctctcc
    24241 tccgcacaac ctacggcggg gactggctat gagctggatg ctattgctgc ggtggttctg
    24301 ggcggtacga gtctggcggg cggaaaaggt cgcattgttg ggacgttgat cggcgcatta
    24361 attcttggct tccttaataa tggattgaat ttgttaggtg tttcctccta ttaccagatg
    24421 atcgtcaaag cggtggtgat tttgctggcg gtgctggtag acaacaaaaa gcagtaataa
    24481 cgactacagg acatcttgaa tatgaacatg aaaaaactgg ctaccctggt ttccgctgtt
    24541 gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag acaccatcgc gctggtggtc
    24601 tccacgctta acaacccgtt ctttgtatcg ctgaaagatg gcgcgcagaa agaggcggat
    24661 aaacttggct ataacctggt ggtgctggac tcccagaaca acccggcgaa agagctggcg
    24721 aacgtgcagg acttaaccgt tcgcggtaca aaaattctgc tgattaaccc gaccgactcc
    24781 gacgcagtgg gtaatgctgt gaagatggct aaccaggcga acatcccggt tatcactctt
    24841 gaccgccagg caacgaaagg tgaagtggtg agccacattg cttctgataa cgtactgggc
    24901 ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg aaggtgccaa agttatcgag
    24961 ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg gcgaaggctt ccagcaggct
    25021 gttgctgccc acaagtttaa tgttcttgcc agccagccag cagattttga tcgcactaaa
    25081 ggtttgaacg taatgcagaa cctgttgacc gcacatccgg atgttcaggc tgtattcgcg
    25141 cagaatgatg aaatggcgct gggcgcgctg cgcgcactgc aaactgccgg taaatcggat
    25201 gtgatggtcg tcggatttga cggtacaccg gatggcgaaa aagcggtgaa tgatggcaaa
    25261 ctagcagcga ctatcgctca gctacccgat cagattggcg cgaaaggcgt cgaaaccgca
    25321 gataaagtgc tgaaaggcga gaaagttcag gctaagtatc cggttgatct gaaactggtt
    25381 gttaagcagt agttttaatc aggttgtatg acctgatggt gacataaata cgtcatcgac
    25441 agatgaacgt gtaatataaa gaaaagcagg gcacgcgcca ccctaacacg gtggcgcatt
    25501 ttatggacat cccgaatatg caaaacgcag gcagcctcgt tgttcttggc agcattaatg
    25561 ctgaccacat tcttaatctt caatcttttc ctactccagg cgaaaccgta accggtaacc
    25621 actatcaggt tgcatttggc ggcaaaggcg cgaatcaggc tgtggctgct gggcgtagcg
    25681 gtgcgaatat cgcgtttatt gcctgtacgg gtgatgacag tattggcgag agcgttcgcc
    25741 agcagctcgc cactgataac atcgatatta ctccggtcag cgtgatcaaa ggcgaatcaa
    25801 caggtgtggc gctgattttt gttaatggcg aaggtgagaa tgtcattggt attcatgccg
    25861 gcgctaatgc tgccctttcc cctgcgctgg tggaagcgca acgtgagcgt attgccaaca
    25921 cgtcagcatt attaatgcag ctggaatcac cactcgaaag tgtgatggca gcggcgaaaa
    25981 tcgcccatca aaataagact atcgttgcgc ttaacccggc tccggctcgc gaacttcctg
    26041 acgaactgct ggcactggtg gacattatta cgccaaacga aacggaagca gaaaagctca
    26101 ccggtattcg tgttgaaaat gatgaagatg cagcgaaggc agcgcaggta ctgcatgaaa
    26161 aaggtatccg tactgtactg attactttag gaagtcgtgg tgtatgggct agcgtgaatg
    26221 gtgaaggtca gcgcgttcct ggattccggg tgcaggctgt cgataccatt gctgccggag
    26281 atacctttaa cggcgcgtta atcacggcgt tgctggaaga aaaaccattg ccagaggcga
    26341 ttcggtttgc tcatgctgcc gctgcgattg ccgtaacacg taaaggcgca caaccttccg
    26401 taccgtggcg tgaagagatc gacgcatttt tagacaggca gaggtgacgc ttggctacaa
    26461 tgaaagatgt tgcccgcctg gcgggcgttt ctacctcaac agtttctcac gttatcaata
    26521 aagatcgctt cgtcagtgaa gcgattaccg ccaaagttga agcggcgatt aaagaactca
    26581 attacgcgcc atcagctctg gcgcgtagcc tcaaactcaa tcaaacacat accattggca
    26641 tgttgatcac tgccagtacc aatcctttct attctgaact ggtgcgtggc gttgaacgca
    26701 gctgcttcga acgcggttat agtcttgtcc tttgcaatac cgaaggcgat gaacagcgga
    26761 tgaatcgcaa tctggaaacg ctgatgcaaa aacgcgttga tggcttgctg ttactgtgca
    26821 ccgaaacgca tcagccttcg cgtgaaatca tgcaacgtta tccgacagtg cctaccgtga
    26881 tgatggactg ggcaccgttc gatggcgaca gcgatcttat tcaggataac tcgttgctgg
    26941 gcggagactt agcaacgcaa tatctgatcg ataaaggtca tacccgtatc gcctgtatta
    27001 ccggcccgct ggataaaact ccggcgcgcc tgcggttgga aggttatcgg gcggcgatga
    27061 aacgtgcggg tctcaacatt cctgatggct atgaagtcac tggtgatttt gaatttaacg
    27121 gcgggtttga cgctatgcgc caactgttat cacatccgct gcgtcctcag gccgtcttta
    27181 ccggaaatga cgctatggct gttggtgttt accaggcgct gtatcaggca gagttacagg
    27241 ttccgcagga tatcgcggtg attggctatg acgatatcga actggcaagc tttatgacgc
    27301 caccattaac cactatccat caaccgaaag atgaactggg ggagctggcg attgatgtac
    27361 tcatccatcg gataacccag ccaactcttc agcaacaacg attacaactt actccgattc
    27421 tgatggaacg cggttcggct tagatttacg ctgtcttttg atcaaattat taccatcggt
    27481 tgttttcaga agcatgaaca ttgctgctga agcaacagta ataatgccca ttgtgataaa
    27541 cgtatagtgg aattgttcga cagtcgttgt accttccatc ccttcataaa cgcgaagaac
    27601 ggccgcactt acagcaacgc ctaaactgat agacagttgc tgcgtgaccg ccagaacact
    27661 gttaccgctg ctggcgttgt catcggtcag atcggcaagt gtgatggtat tcattgcggt
    27721 aaattgcgtc gacatagcca tccctaatat aaacaacggc aagatcagca tccatatcgc
    27781 cattgctggt gattgcaaag agaactgagc gatcattagc ccaataatca ccgtgatccc
    27841 cactaacgta tggcgatagc ccagacgacg taagacttgg gtaaccatcg attttgcaat
    27901 aatggaacct aacgctgtcg gtgccatcat acagccggca ataaacgcct gataaccaaa
    27961 tcctacctgt aacatcaatg gcataaggaa cggtacacag ccggtcccca gacgggttgc
    28021 aatattgcct acgataccga tcgagaaagt gcgggtttta aataaatcta atgaaattaa
    28081 tgggtttggc gtgcgtcgtg catggagaat atagagaagc agtaacccga tgctggtgac
    28141 aattaccgtc aaggcaatcc aactggcgac aatcttttcc ccgaatagct ctattccgct
    28201 tgagaagaga acaaggctaa ggccaaacag caaaaagcca gtgatatcga atctgcgtcg
    28261 tgcggtggtg aaattgggca tatgtttgcg cgcgtaaaga aggcccgcaa tacctatggg
    28321 gatattgatt aaaaatatcc agtgccaggt tgcccaggtg accagcacgc cgccaagaac
    28381 ggggcctaaa attggcccca ccagacccgg catggcgaca aaattcaata caggaagaag
    28441 ctcattacga ggataagcgc gcagtaaggc cagccgagca acaggcatca tcattgcgcc
    28501 gcctatcccc tgaataaccc ggaagacaac cagctgtggt agcgagttag aaagtgcgca
    28561 ggccagagaa cccaacgtga acagactcac ggcaagggta aatatgcgac gcgtaccgaa
    28621 gcgatcggct agccatccgc ttaccggaat aagcatcgcc accgtcagcg tataactgat
    28681 gatggctgat tgcatcgcga gaggagaacg attaaggcta tgagcgattg cgggtaaggc
    28741 ggtattcaga atagtggcat caagtgcctg catgaagaag gccatcgccg cgatccacgg
    28801 caaacccgcc atactgcgct tctttttatc gctcattcaa tgtcctgtta tcgggttgtc
    28861 acttatcagg tgagcgtagc agcgcctgac aagctttaaa tgccgcgtcg ccatcgcttt
    28921 ggacaatcgc atcgacaatc gcctggtgca gatccagctt tatcactgtg tcgctggtaa
    28981 ttgacgtgaa gtaagtgtga taaaccgaat ggaatagcga ggcgaatgag gtcaaaaacg
    29041 gattggcgct catttcatag atatgctcat gccaagccat atcgacctca atccagcgtt
    29101 cacggcgaaa gttctctttt aatgccacca tttcggccat taacgtattg agatgcgcct
    29161 tctgttccgc ggtgccaacc gttgcagcca gtaggcaagc ttgcggctcc agacagatac
    29221 gcataaccag aaagtgatcg atgacctgat gaaagttctc ttctgtcatc caccaggtaa
    29281 gcaattcctg atcaagaaaa ttccagtttg attgtggcat gacccgggta ccaattcgcg
    29341 gtcgcggtaa aaccatccct tttgccgtta acgttttgac cgcttcgcgt accgctgtac
    29401 gactcactcc aaattgctcg cccagctcaa tctcaccagg caaaatggtg ccgggttcat
    29461 attcaccttt taagatgcgt tgcgccagct tctcagccag aacatacgaa aggtttttct
    29521 gtgcagctaa ctgttgtgcg cttaaaggca ttactaatct tcctttttct ttttattcct
    29581 ccttagtatg ccaccaggaa gtgtgattac ggttgcaaaa acggcaaatt gcttgtttta
    29641 tggcacatta acggggcttt tgct
//
LOCUS       DAYPHN010000044        29172 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000044 DAYPHN010000000
VERSION     DAYPHN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.044"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..592
                     /locus_tag="ACG0BW_001930"
     CDS             <1..592
                     /locus_tag="ACG0BW_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFK7258898.1"
                     /translation="FGLMLTLCSSVMGVLAGALQGYYGGKVDLWGQRFIEVWSGMPTL
                     FLIILLSSVVQPNFWWLLAITVLFGWMSLVGVVRAEFLRTRNFDYIRAAQALGVSDRS
                     IILRHMLPNAMVATLTFLPFILCSSITTLTSLDFLGFGLPLGSPSLGELLLQGKNNLQ
                     APWLGITAFLSVAILLSLLIFIGEAVRDAFDPNKAV"
     gene            594..2183
                     /gene="yejF"
                     /locus_tag="ACG0BW_001931"
     CDS             594..2183
                     /gene="yejF"
                     /locus_tag="ACG0BW_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFK7258899.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(2187..2531)
                     /locus_tag="ACG0BW_001932"
     CDS             complement(2187..2531)
                     /locus_tag="ACG0BW_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFK7258900.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(2864..4054)
                     /gene="bcr"
                     /locus_tag="ACG0BW_001933"
     CDS             complement(2864..4054)
                     /gene="bcr"
                     /locus_tag="ACG0BW_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFK7258901.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(4082..4777)
                     /gene="rsuA"
                     /locus_tag="ACG0BW_001934"
     CDS             complement(4082..4777)
                     /gene="rsuA"
                     /locus_tag="ACG0BW_001934"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFK7258902.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            4927..6687
                     /locus_tag="ACG0BW_001935"
     CDS             4927..6687
                     /locus_tag="ACG0BW_001935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFK7258903.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            6812..7096
                     /gene="rplY"
                     /locus_tag="ACG0BW_001936"
     CDS             6812..7096
                     /gene="rplY"
                     /locus_tag="ACG0BW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFK7258904.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(7235..8242)
                     /gene="yejK"
                     /locus_tag="ACG0BW_001937"
     CDS             complement(7235..8242)
                     /gene="yejK"
                     /locus_tag="ACG0BW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFK7258905.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            8424..8651
                     /locus_tag="ACG0BW_001938"
     CDS             8424..8651
                     /locus_tag="ACG0BW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFK7258906.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            8671..10431
                     /gene="yejM"
                     /locus_tag="ACG0BW_001939"
     CDS             8671..10431
                     /gene="yejM"
                     /locus_tag="ACG0BW_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFK7258907.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            10506..10582
                     /locus_tag="ACG0BW_001940"
     tRNA            10506..10582
                     /locus_tag="ACG0BW_001940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10540..10542,aa:Pro,seq:ggg)
     gene            complement(10685..13276)
                     /gene="yejO"
                     /locus_tag="ACG0BW_001941"
     CDS             complement(10685..13276)
                     /gene="yejO"
                     /locus_tag="ACG0BW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFK7258908.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANEPTIKGGRLIIEKDGITVFAAIEKGGLLEVKEGG
                     LAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKDGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTPLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYSSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            13596..14243
                     /gene="narP"
                     /locus_tag="ACG0BW_001942"
     CDS             13596..14243
                     /gene="narP"
                     /locus_tag="ACG0BW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFK7258909.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(14278..15330)
                     /gene="ccmH"
                     /locus_tag="ACG0BW_001943"
     CDS             complement(14278..15330)
                     /gene="ccmH"
                     /locus_tag="ACG0BW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFK7258910.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTM
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15327..15884)
                     /gene="dsbE"
                     /locus_tag="ACG0BW_001944"
     CDS             complement(15327..15884)
                     /gene="dsbE"
                     /locus_tag="ACG0BW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFK7258911.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(15881..17824)
                     /gene="ccmF"
                     /locus_tag="ACG0BW_001945"
     CDS             complement(15881..17824)
                     /gene="ccmF"
                     /locus_tag="ACG0BW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFK7258912.1"
                     /translation="MMPEIGNGLLCLALGVALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAV"
     gene            complement(17821..18300)
                     /gene="ccmE"
                     /locus_tag="ACG0BW_001946"
     CDS             complement(17821..18300)
                     /gene="ccmE"
                     /locus_tag="ACG0BW_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFK7258913.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(18297..18506)
                     /gene="ccmD"
                     /locus_tag="ACG0BW_001947"
     CDS             complement(18297..18506)
                     /gene="ccmD"
                     /locus_tag="ACG0BW_001947"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFK7258914.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(18503..19240)
                     /gene="ccmC"
                     /locus_tag="ACG0BW_001948"
     CDS             complement(18503..19240)
                     /gene="ccmC"
                     /locus_tag="ACG0BW_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFK7258915.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(19282..19944)
                     /gene="ccmB"
                     /locus_tag="ACG0BW_001949"
     CDS             complement(19282..19944)
                     /gene="ccmB"
                     /locus_tag="ACG0BW_001949"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFK7258916.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(19941..20564)
                     /gene="ccmA"
                     /locus_tag="ACG0BW_001950"
     CDS             complement(19941..20564)
                     /gene="ccmA"
                     /locus_tag="ACG0BW_001950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFK7258917.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(20577..21179)
                     /gene="napC"
                     /locus_tag="ACG0BW_001951"
     CDS             complement(20577..21179)
                     /gene="napC"
                     /locus_tag="ACG0BW_001951"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFK7258918.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(21189..21638)
                     /gene="napB"
                     /locus_tag="ACG0BW_001952"
     CDS             complement(21189..21638)
                     /gene="napB"
                     /locus_tag="ACG0BW_001952"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFK7258919.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(21635..22498)
                     /gene="napH"
                     /locus_tag="ACG0BW_001953"
     CDS             complement(21635..22498)
                     /gene="napH"
                     /locus_tag="ACG0BW_001953"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFK7258920.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(22485..23180)
                     /gene="napG"
                     /locus_tag="ACG0BW_001954"
     CDS             complement(22485..23180)
                     /gene="napG"
                     /locus_tag="ACG0BW_001954"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFK7258921.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(23187..25673)
                     /gene="napA"
                     /locus_tag="ACG0BW_001955"
     CDS             complement(23187..25673)
                     /gene="napA"
                     /locus_tag="ACG0BW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFK7258922.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(25670..25933)
                     /gene="napD"
                     /locus_tag="ACG0BW_001956"
     CDS             complement(25670..25933)
                     /gene="napD"
                     /locus_tag="ACG0BW_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFK7258923.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(25923..26417)
                     /gene="napF"
                     /locus_tag="ACG0BW_001957"
     CDS             complement(25923..26417)
                     /gene="napF"
                     /locus_tag="ACG0BW_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFK7258924.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQTVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            26526..26690
                     /locus_tag="ACG0BW_001958"
     CDS             26526..26690
                     /locus_tag="ACG0BW_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258925.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHTYIAINSPEI
                     FMSVCSLTRI"
     gene            26804..27313
                     /gene="eco"
                     /locus_tag="ACG0BW_001959"
     CDS             26804..27313
                     /gene="eco"
                     /locus_tag="ACG0BW_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFK7258926.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(27461..29107)
                     /gene="mqo"
                     /locus_tag="ACG0BW_001960"
     CDS             complement(27461..29107)
                     /gene="mqo"
                     /locus_tag="ACG0BW_001960"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFK7258927.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QVSGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT         7080 a         8163 c         7225 g         6704 t
ORIGIN      
        1 gtttggcctg atgctgaccc tctgctccag cgtgatgggc gtgctggcgg gggcgctaca
       61 aggctattac ggcggtaaag tcgatctctg ggggcaacgt tttattgaag tatggtcggg
      121 gatgccgacg ctgtttttga ttattttact ttccagcgtc gtacagccta acttctggtg
      181 gctgttggca attaccgtct tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga
      241 gtttttacgt acccgtaatt tcgactacat ccgcgcggcg caggcgcttg gcgtcagcga
      301 tcgcagtatc atcctgcgtc atatgttgcc aaatgccatg gtcgctaccc tcaccttttt
      361 accgtttatt ttatgtagtt cgatcaccac cctgacctca ctcgatttcc tcggcttcgg
      421 tctgccgctc ggttcaccgt cactcggcga actgctgtta caagggaaaa ataaccttca
      481 ggccccgtgg cttgggatca ccgccttctt gtcggtggcg atattgttgt ctttgctgat
      541 ctttattggt gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc
      601 aaactctgtt agcgattgaa aatttgtcgg tgggttttcg ccatcagcaa accgtacgta
      661 cagtagtcaa tgatgtttca cttcagattg aggctggcga aacgctggcg ctggtgggtg
      721 agtcaggttc aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc
      781 cggttgaata tctctccggc gatattcgtt ttcatggcga atcgctgctt catgccagcg
      841 atcaaacgtt gcgcggtgta cgcggtaata agatcgccat gatttttcag gagccgatgg
      901 tgtcattaaa tccattgcat accctggaaa aacagcttta tgaagtgctt tcactccacc
      961 gcgggatgcg tcgggaagcg gctcgtggcg aaattctgaa ctgccttgat cgcgtcggta
     1021 tccgccaggc ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc
     1081 agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac
     1141 cgaccaccgc gctggacgtc tctgtccagg cgcagatttt acagctgttg cgcgaactgc
     1201 aaggcgagct gaatatgggc atgctgttta ttactcataa cctcagcatt gtcagaaaac
     1261 tggcccaccg cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta
     1321 cgctatttgc ctcacccact catccttaca cacaaaagct actcaacagt gaaccgtcag
     1381 gcgatccagt gccgttgcca gaacctgcct caacgttgct ggatgttgaa cagcttcagg
     1441 ttgccttccc cattcgcaaa gggattttga agcgcattgt ggatcataat gtggtggtga
     1501 aaaacatcag ttttacgcta cgagcgggtg aaacactggg tttagtgggc gagtccggtt
     1561 ccgggaaaag cacgacggga ctggcgctgc tgcgactgat taactctcag ggcagcatta
     1621 tctttgacgg tcagccactg caaaatttaa atcgtcgcca gctgttacct attcgtcatc
     1681 gcattcaggt ggtatttcag gatccaaact cctcgctcaa cccacgactc aacgttttgc
     1741 aaattattga ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac
     1801 aacaagtgat agccgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc
     1861 cggcggagtt ctctggtggt cagcggcaac gtattgcgat tgccagggcg ttaattctta
     1921 agccctcgct gatcattctt gatgaaccaa catcatcgct cgacaaaacg gtacaggcgc
     1981 aaatattgac gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca
     2041 gtcacgattt gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg
     2101 aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccgc accgcagcag gagtatacgc
     2161 gtcagctact ggcgttgagc tgacgcttaa aaaggattgt agtttgagaa gggttcggca
     2221 attgccacac caaaattttt caatcggcat gttgcagcaa actcgtcctg gcgatttaca
     2281 aacaggcacg gctcaccttc acactccagt accgaacatg gaacttcgat gtcgcttaac
     2341 gcctggctga gtttgtacat caccgaccat gcattatcac catccgggct aagcaattta
     2401 agcgccacca ggctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa
     2461 cctgcaaaac cagcagacca gcgatagttc tgcggcagtc gatggacgat tgagagttgt
     2521 aatgaagtca cttattttcc ccggaagtac atttacttca caatttgttt acatcaattt
     2581 taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgccagcgt
     2641 ggcctctatg gctctgattt aaataatatc tgcgggcgtg gtcacgtttg cggggctata
     2701 tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat
     2761 aaattgattt tacataaaat aaacatatat cgggggaatg atggatttat ggttgacatg
     2821 catcaacaaa ggaagccttt tagcttcctc gttgtgcaat agatcaccgt ttttttggcc
     2881 gactggcgta cagacagaag agaatggagc tggttgcgca gaatgcaatt gaccaaatca
     2941 tcggccatgc agagttaaag gtcgcaagag aaagcaatgc gccaacaatt gcccctatgc
     3001 cgaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa
     3061 gaatgaccgc catcgcatta gatgacacca tcgacacgca gcccacaaac gccgcaacgc
     3121 caaccaccag cgaccagaat cccagcccca gcagcgcact gatgaccatc cacgccgcca
     3181 taataaactg tatccacaac cccgagcgga acatatttaa cgcgccaata cggcggacga
     3241 agcggctgtt aaagatggtc atcacgaaca ggaaaacaat gtttagcgca aagtaataac
     3301 caaagttttc cggcgcgacg tggttaattt caatgtagac aaacggtccg gcgctcagga
     3361 acgagaacat cccggcaaag ctgaaaccgc tggccagcat ataactcagg acacgcttat
     3421 gacggaacag cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc tgacgacgct
     3481 ctggcggcaa cgtttcttta atcaggaaga aaatcattgc cgaagccaga atcgccgcta
     3541 cggccaggat ccagaagatg taatgccagc tcagccacac cagcacccaa ccgccgacaa
     3601 tcggtgccat caacggtgca atagtggtca ccagcatgac aaacgacatc atccgcgaga
     3661 actcttcttt cgggtaaata tcgcgcatca gggcattaat gaccacgctg gccgcagccg
     3721 cagccagccc gtggaagaaa cgcatcacaa tcagctgatc gatggtgttt gccaacgcac
     3781 acgccaccgc ggcggcggca aacaccagcg taccgccaag aaccaccggc ttacgcccga
     3841 agctgtctgc catcggcccg tagattaact gccccaacgc aaagcccaga atataagtac
     3901 tgagggtcat ctgcgtactg cccgccggta cgccaaactg cgctgaaatt accggtagcg
     3961 cgggcagata catatcaatc gacagcggca tcaacatggc cagcaggcca aggataaaaa
     4021 caatagcaaa cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa
     4081 gttagacgac gctggcaatt tcttcttcag ttaacggacg atattcaccg ggggctaaat
     4141 cagcatccag cgtaatgccg ccaatacgtt cacgatgcag ctcaaccaca tggttaccca
     4201 cggcggcgaa catacgtttc acctgatgat aacgcccttc gctgatggtc aaacgaacct
     4261 gcgttggggt aatcacttcc agcaccgccg gcttagtgag atctttttcg ttatgcagct
     4321 gcacgccttt agcaaattgc tctgccgtat cgtcagctac gggtgattcc agcgtcacca
     4381 gataggtctt ctcacaatga tggcgcggag aagtaatgcg gtgcgaccac tgaccatcat
     4441 ctgtcatcag caccagaccg gtggtatcga tatccagacg ccctgcggcg tgcagtttcc
     4501 acgctaccgg ttcatccaga aaatagagca ccgttgggtg atcagggtcg tccgtggagc
     4561 aaacatagcc ctgaggctta ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg
     4621 ggttgccatc gtaagcgaca tcatgttcag ggagcagttt gaacgctgca ttacggacga
     4681 tttcgccatc gacggtgaca cgattgccgc ggatttcacg cccggcaata gcacggctaa
     4741 cgccgagttg ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa
     4801 acggaggtcg ggtaatgacc cgaaaatctg aactgttgtc tgcccagtat agcggtctaa
     4861 taacttccct caagggaaaa agatccgtgg catactatta gcagaataat ctacctacgc
     4921 gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc cacgctcaac
     4981 cattttcgtc gtcataaaac ccctgccgtt atcgtgctgc ccaccggtgc aggtaaaagc
     5041 ctggtgatag cggaactggc gcggctggcg cgtggtcgcg tgctggtgct ggcacacgtt
     5101 aaagaactgg tggcgcaaaa ccatgcaaaa tatcaggcgc tggggctgga agccgatatt
     5161 tttgccgccg ggctaaagcg caaagagagc cacggtaaag tggtatttgg cagcgtgcag
     5221 tcggtcgccc gtaatcttga tgcctttcag ggtgaattct cgctgttgat tgtcgatgaa
     5281 tgtcaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac tcacctgacc
     5341 aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg actgggcaaa
     5401 ggctggattt atcagttcca ttatcacggc atggtacgcg gggatgagaa agcccttttc
     5461 cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta tctgacaccg
     5521 ccagaacgac tggatatgcc agtagtgcaa tacgatttca gccgcttgca ggcacagagt
     5581 aacggtttgt tcagtgaagc cgatctcaac cgtgagctga aaaaacagca acgtattacc
     5641 ccgcacatca tcagccagat tatggagttc gccgaaaagc gtaaaggagt gatgattttc
     5701 gccgccaccg ttgaacacgc caaggagata gtgggactgc tgcctgccga agatgctgca
     5761 ctgattactg gcgacacacc cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc
     5821 cagcgttttc gctatctggt caacgtcgcg gtactgacca ccggatttga cgccccgcac
     5881 gtcgatctta tcgccattct gcgccctacc gaatcggtga gtctttacca acaaattgtc
     5941 gggcgcggtc tgcgtctcgc tcctggcaag actgattgct taattcttga ttatgcgggt
     6001 aatcctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtc
     6061 ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc
     6121 gctgacggga cattgattga acactttggt cgtcgctgtc agggatggtt tgaagatgac
     6181 gacggtcatc gagaacagtg tgacttccgt ttccgtttta aaaattgccc gcaatgtaac
     6241 gcagaaaatg atattgccgc ccgccgctgc cgcgaatgtg acacggtact ggttgatccg
     6301 gacgacatgt taaaagcggc gctacgactg aaagacgcgc tggtattacg ctgtagcggc
     6361 atgtctttgc agcatgggca cgatgagaaa ggcgaatggt tgaaaatcac ctattacgat
     6421 gaagacggcg cggatgtgag tgagcgtttc cgtctacaaa cgcccgccca gcgtaccgcc
     6481 ttcgaacaac tttttatccg tccgcatacg cgcacaccgg gcatccctct gcgctggatc
     6541 accgccgccg atatcctcgc ccagcaagca ttactgcgac acccggattt tgtcgtcgcc
     6601 cgcatgaaag gtcagtactg gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt
     6661 cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag
     6721 tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc
     6781 ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa
     6841 agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat
     6901 ctacggtggc aaagaagcgc cgctggctgt cgaactggat cacgacaaag tcatgaacat
     6961 gcaagttaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat
     7021 caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc tgcacatcga
     7081 cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt
     7141 gtctctgcaa attatgcctg attacggtat tgctattttt tgcaggccag ataaggcgtt
     7201 cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccagac gtccggcgct
     7261 gcaattgatc gcgcaaattc ggcggcgtgc ctttaatggt tagggtatcg gttgccggat
     7321 cccagaagat gcgttcacct aacagcatcg catcaaagtt aatagtcaag ccaccgccgc
     7381 taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag ctctcttcca
     7441 gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgctgacg cctgccagct
     7501 ctttcgacag cgactccagc tcaatctctt cgcctgcctg cagctgctca ttacagtagc
     7561 tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga
     7621 aatcatcgac cgcttgcaac agcccacggt tttgcgcttt cgcgtttaac ccttcgctgg
     7681 ccccgaggaa atccataaag aaatccgcca ctttgcgccc tactcgccct ttcaggaaag
     7741 tgagatagcg ggtcgactct ggattggttt cccactcggt taaatcgata cgcgcaacaa
     7801 tatccgcatg attgatatca agataatggg ttgggttgat gtcgagattt tcgttaacac
     7861 gcatactgct caggttactc agaactgcca ccagcagata ctccaccgcc agatagcgat
     7921 agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca
     7981 ggcgaccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc
     8041 gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc
     8101 tatagacccg atgcagttct gccaccatct caacgacggt ttccgtcggt tccagcaatg
     8161 aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg tgcagagcaa
     8221 tctggttgat atccagactc atgataaact ctcctttaag accgggcggt attcaaccac
     8281 cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcag cgtaacgaga
     8341 atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt
     8401 catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg
     8461 aacaattgct cgcagagctg ctcaacgtac tggaaaaaca taaggctccg accgaccttt
     8521 ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc
     8581 aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc aacgaagaca
     8641 aagcgcacta agggaaacag ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa
     8701 aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg
     8761 ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct tgctggtcgc
     8821 atttattcct acgtaagcat tatcggccat ttcagcttcc tggtgttcgc cacctacttg
     8881 ctgatcctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc
     8941 gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt ctttactcgt
     9001 ttccatctcc atcttaatcc catcgtctgg caactggtta tcaacccaga cgaaaatgag
     9061 atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaactg
     9121 gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg gcgcttcgcg
     9181 cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt gtatatctgg
     9241 gccgatgcca acttctatcg cccgatcacc atgcagcgcg ctaacctgcc gctttcgtac
     9301 ccaatgacgg cgcgacgttt tcttgagaag catggtctgc tggatgcgca ggaatatcaa
     9361 cgccgtctca ttgagcaagg taatccagac gccgtttccg ttcagtatcc gttaagcgaa
     9421 ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt cgatggcctg
     9481 aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt
     9541 tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt tggcctgttc
     9601 tatggcatct cgccgagcta tatggacggc attctgtcga ctcgtacgcc tgcggcgtta
     9661 attactgcgc ttaatcagca aggctatcag ctggggttat tctcgtcgga tggctttacc
     9721 agcccgctgt atcgccaggc attgttgtca gatttctcga tgccgagcgt acgcacccaa
     9781 tccgacgagc agaccgccac gcagtggatc aactggctgg gccgctacgc acaagaagat
     9841 aaccgctggt tctcgtgggt ttctttcaat ggcactaaca ttgacgacag caatcagcag
     9901 gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg
     9961 ctcaatgcac tacgtgattc tggcaaactg gacaatacgg tggtgattat cactgccggt
    10021 cggggtattc cgctgagcga agaggaagaa acctttgact ggtcccacgg tcatctgcag
    10081 gtgccattag tgattcactg gccaggtacg ccggcgcagc gtattaatgc tctgactgat
    10141 cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc
    10201 gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg ggttaccgcc
    10261 gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac
    10321 ggtaaatacc gtacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta
    10381 agtttgttat tgcaagtact gacagacgag aagcgtttta tcgctaactg attaattata
    10441 aatcagttag caaaatatct tacttgcaat cggtgtggaa aacggtagta ttagcagcca
    10501 cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt
    10561 tcaaatcctc tcgtgccgac caaaaatccc aaaaaaaacc aacctttacg gttggttttt
    10621 tttatatctg catttaattc gataaacaga ccgcgacaca tcacggcctg tttattttct
    10681 gttatcagaa cgtccagacc acacccgcct gagtattcca cggcgattct acccctgcgc
    10741 cattgccata gctgactgac aaatgaccgc ttaacgtcgg ggtgaacgat gaccttatcc
    10801 cagcctgata aacgccacgg gttccagcca catcattatt aaatttgcca tcgtcattaa
    10861 ctttcacctg gttagaatcg gcgtattcct gacgtacggc ggctttcagc cagggttcca
    10921 gcgtcgtacc gttttgcagg tccatgtgat agcttaccgc cgttcccgct tcagcgcgta
    10981 atatgcgggt atttcccaca tccgcgcgca tgccgtttga taacgtgtag tcctgaccat
    11041 ctgtggtaaa gccggtaaag gccagataag gtctaacact ccacaatccg tcaacccaac
    11101 ggaacccgct ctcgacatga gcacccgcgc cgttactact gtaatcgcca aacgctgttg
    11161 ccccattact catcttgcca tggatggtgt tggcaaaacg gtcaactttc accactccat
    11221 caacataggc accgttctga tgctcccaac cggcataagc ccccagggtg tagctatcga
    11281 cattgccttt gccgccgcga tcaaaaccaa tatcagaatg agagtaacca aagaacaagc
    11341 cgcgaattgt gctgctttct tcacgggaga aacggctatc gataccgagc gtcaggcccg
    11401 tcaatgtttg ctcaaagcca gctcccgcat cagtggtcac gttgttgcgg gtgttaattg
    11461 ccgaactcca catcgccgta tcgtaactaa cgccttttac gctaccaaga cgctcacgca
    11521 cggtgtccag ttctgcatca aataccagcg gttgtgctgc tgccatgttc agcacatcag
    11581 tggttgaagg ggtaatttgc gcgcgattct ctgcgaggct ccagctatgg ttgccattat
    11641 ccagcaaggt atattcatac gtaccgatat caacaacgcc tccggcattg cccaacgtaa
    11701 atgcagcatc accgccgccc gttgttacca gtgtaaggct atctcctgct gccgggctgg
    11761 caccggtgtc cgtcacgaat attttgaaat cacctgttgc ctgcccggtg acgttgagct
    11821 gatcgctctg atgattagcc atatcggtac gcatataaaa attgccgttt cccgataggg
    11881 tattggtggt caacgtagtg aaattttccg cacttgccgt cacagatgaa cgagtcactg
    11941 gctcagccat cataatcaca tttgcgccag acatcgcaag gttggctaag ttgaggggag
    12001 tgttttggtt cgtcgtagtg atatcggcaa tgatggtcca tgcgctattt tcgagtgaaa
    12061 catccgcttt gctggtatcc acggcaccat gcgttcttaa agaagcgccc tcagaaacca
    12121 ctaccttacc caccaacata gcgggtgcat aatttatttg cggcttcatg acctcaagaa
    12181 tgccatcatt ccctctgact gaaacgttaa ccattgtgcc agcccagaca ttagcgcggc
    12241 cattgttgat aaccatattt tcagagatgg ctttagagga atacgtgata cttccattct
    12301 gatctgatcg accgagatca tacaccgcat ttgcctgcac tctcgtacca gtatcctttc
    12361 ccagcgattg catagtggca tgctcatcaa ggatagtgtc aatggcctgc gtgtcctcca
    12421 taacaataag cccggaacca tcatccagct cataattttt tgacacacca tcttttatac
    12481 taaattggcc tttactgttt gtaccactca cttccagcgc attcgttgag acaattaatt
    12541 ttccgcctgc ttttttatca acgccagttg cagtcccgcc atccataacc tccagtaagc
    12601 cgccactatc cacagtggta ttataagcga agtcattttc ttcaactcgc aaacttccgc
    12661 cgttttccaa cagcatatta ttagcaatac catccttgat atcgaactga ccgagacggt
    12721 ttgttccgaa tacctccatg gcccgggtgg ttgttttaat cgcaccgcct gctttctgat
    12781 ctaccgcaaa tgctaatccc ccctctttaa cctccagtaa tcctcccttt tcgatagcgg
    12841 caaagacggt aatcccatct ttttcaataa ttaagcgccc gcctttaatc gtaggttcat
    12901 tcgctgcgcc ataaacctgt agcacaccat tattgagggt agtctggttt gccgcaccac
    12961 ccatttgaac aatttgataa ccatcattta atactgagta ttctgcggtg ccattcattt
    13021 caatgtactg atacccgccg ttaatttcag tattactact tataccaaat tctttaatat
    13081 gctgttctcc gccaggattg attttcgtat tattggcggt cccataaacc agctggatat
    13141 ctttttccag gacaacacca tcaactgtct caccatactc aactgttgat gccaatgcta
    13201 tgggtgaata taacgctgta gccaccagaa cagatattgc ggaacgacaa agagaaacag
    13261 aaccagattg atgcattgag ctttcatcct atgaaattaa ttgctgttaa aagcattggg
    13321 taaagaaaat acccatagct ccatacccgg agtcagtttt taaaaactgt ttaaagaaat
    13381 gcacaagtat tgtgattgat tttttagttg tttttcttga tgagaagctg atgcaaaatt
    13441 ccgtctttat aatgaaaatg atgccaaagc gaacgacaag gttgtagttt tcactacatg
    13501 tccatacata aaatggggta acattcacgc gcctggtagc gttaccaacg ctacgctcaa
    13561 acataatgat tctaataaaa cctcaggaga ctactatgcc tgaagcaacg ccttttcagg
    13621 tgatgattgt ggatgatcat ccacttatgc gacgcggtgt tcgtcagtta ctggagcttg
    13681 atcctggctt tgaagtggtc gccgaagcgg gcgacggcgc gagcgcgatc gatctggcga
    13741 atagactgga tatcgacgtg atcttgctgg atctcaatat gaaaggtatg agtggcctgg
    13801 ataccctcaa tgccttgcgc agggatggcg ttaccgcgca aattattatc ctgactgtat
    13861 cagatgcctc cagcgatgtc tttgcgctga tagacgcagg cgcagacggt tatctgttga
    13921 aagatagcga cccggaagtg ttgctggaag cgattcgtgc cggagcgaaa ggcagcaaag
    13981 tctttagcga acgcgtcaat cagtacttac gtgaacgtga aatgtttggc gcggaagaag
    14041 atcccttcag cgtgctgacg gagcgcgagc tggatgttct gcacgagctg gcacaggggc
    14101 tgtcaaataa acagattgcc tcggtgttga atatttccga gcagacagta aaagtacaca
    14161 ttcgcaatct gctgcgtaaa ctcaatgtcc gctcacgcgt ggcggccacc attctgttcc
    14221 tgcaacaacg cggggcacaa taaaaatagc ccgatggcat caccatcggg cctcttttta
    14281 tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg atactacgtt caatcactgc
    14341 acggcgagta tcgttggcag gtaagagttt caacatcatc tcccacgcgg caaccgcttc
    14401 gccaaatcgc tgctgctcaa aggcattaaa cgcatacatg cttaacacac ggatattgct
    14461 atggtccgtt ctcaccaact ggcgtagcag ttcaccaccg aggcggttgt cgttgggatc
    14521 agatgaacgt gtcaacgctt cagcgtatcc gagcgccgca tcactgttct tcggatcgag
    14581 gcgatatgca gtagcgtatg catcggtggc aatactggcg ttacccagcg ccatgccaac
    14641 gcggcccaac ataatccagc cttctatatc tcccggattt ttttgcagtt gagtacgcat
    14701 ccccagcgcc aggcgcgaca tctcttcttc gttgagcgga tcggctttcg gatccagcgc
    14761 cctgtccagt aacgccggag cctgtgccgt ggcctgctgc cagattttca cctgctgata
    14821 attgcccgtc tggtagtagc tgacgccagc cacaattaac gccaccacaa tacccggcag
    14881 ataaacaata tatccggcac gcttaccttc cggcacgctt tgttcaggaa acgcgtccgg
    14941 caccacgcgt acccgacgcc gcgagcgggc gtatatgacc caaccgccaa tgccaatagc
    15001 cactaccgga agcacccaca gcagcatggt cagcggcgtt aacggcggat cgtaagtgac
    15061 gaagttgccg taacgcgcca ccatataatc gacaatctct tttttacttt taccttcctg
    15121 catcagttca tacactttct ggcgcaggtc ggtggcaatc atcgagttgg aatcggcaat
    15181 gctgttgttc tggcatttcg ggcagcgtag ttcttcagtg agttgccgga actgctgctc
    15241 ctgcgcttca tccttaaact gcaacacgtc gatggtcgcc agcgctgagc cggagatcat
    15301 cagcatcagc acgcccaata aaaacctcat tgtgcggcct ccttactgta tttctcccac
    15361 agcggcttga tctcttcttc ccagacgcga ggattgagat cgcccgcatg gcgatagcga
    15421 atgatgccgt tgccgtcaat aaggaacgtt tctggcgcgc catagacacc gagatccagt
    15481 cctaacatgc cgtcgccatc aaacaggctt agtgcgtaag gattgcccag ctctttcagc
    15541 cagctgattg ccttctggcg atcgtcttta tagttcatgc cgaccacgcg gatgccctgc
    15601 gcagaaagct gattcagata ttgatgttcc gcacggcagg tcggacacca ggtcgcccag
    15661 acgttaagca gcactggttt gccctgagtc agcacatccg cctgataaaa ctgccccgga
    15721 ttgtccagtg attcgaggcg aaacttcggc accggcttgc caatgagcgc cgattccaga
    15781 ttggtcggat catccccttc ggcattacgc gccagctgcc acagtagcgc cgcggcaatc
    15841 gccaggaaga taatcaacgg aattaacaat actttgcgct tcatacagcc tccggcgcag
    15901 ttttctgcgg gttcacgcgc ttacgatagc gaggatcaaa caggcacagc agcccgccca
    15961 atgccatcat cagcccgccc gcccaaatcc agcgaacaaa cggtttgtag taaagacgca
    16021 cggcccacgc gccgttttcc agctcttcac cgagcgcggc gtacaggtca cgcgtgatgc
    16081 cgccgtcaat cgccgcttcg gtcatcatcg acccggcagt gttgtaataa cgtttttccg
    16141 catacagcac cgtttccggc ttgccatcgc gcgttacgcc gatagtcgcc acaccgccac
    16201 gccagttcgg gccagtcacc tctttgacat cacggaaggt gaagcgatat tcatgaatat
    16261 cgacactatc gccggacttc atgcgcacat cacgctcaac gctatagttc tggctaaagg
    16321 caataccaac aattgtcact gccagcccca ggtgagccgc caccattccc caataactga
    16381 aggtggtttt cgtgccacgt gaaatacgca gcgcagcttc cgcaattgcc agcaccgcaa
    16441 tccagcaggc cattgccagg ccgagcaccg tcatcgccac aactttgctt tcgaacagcc
    16501 acggcagcag cagcgacagc accagcgttg agatgaaggc gataatcaat aaattgcgga
    16561 ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc gacaccaagc agcagcgcaa
    16621 acggcaccat cagccaggta aacatggtgt tgaagaacgg ttcgccaatc gaaatactgc
    16681 ccagtcccag ttgcttgtgc accagcggca gcagcgtccc cagtaatacc accagcatcg
    16741 cggcgaccag caaaacgttg ttcgctaaca gcagagattc ccgcgaccac agcgcattgt
    16801 ttacgcgtga gcgaactttg tgtccacgcg cggcaaaaag cagcagcgaa ccgccaatca
    16861 ccagcaccat aaaggcgagg ataaacatac cgcgtgccgg atcagacgca aacgcgtgta
    16921 ccgataccag tacgccggaa cgcaccagga aagtccccag cagacacaac gagaaggcac
    16981 tgattgccag cagtaatgtc cacgctttga agctggcgcg ttgttcagtg accgccagtg
    17041 agtgcatcag cgcagtcccc accagccacg gcataaacga ggcgttttct accggatccc
    17101 agaaccacca gccgccccag ccgagttcgt aataggccca tgcggaaccg agcacgatgc
    17161 cgagcgtcag gaagatccac gctgccagtg tccacggacg agtaaaacgc gcataagtgc
    17221 tgtccagacg cccgctcaac aaagaagcaa tggcaaaagc aaacgccacc gagaagccga
    17281 cgtaccccat ataaagcaga ggcggatgga agatcagccc cggatcctgc aacagcgggt
    17341 taagatcgcg accttcaatc gggaaattcg gcagcgtgcg tgaaaacggg ttagaggtaa
    17401 agagaatgaa cagcaaaaag ccgacgctga ccatccccat tatcgccagt acgcgggcca
    17461 caatatccag cggaatacgc tgactaaaaa tcgccaccgc aaaggtccag ccgctcatca
    17521 gcagcaccca cagcaggagc gagccttcat gtgcgcccca ggtagccgcc acgcgatacc
    17581 acaccggaag ctgggtattg gagttgctgg caacataggt gacggtaaag tcgttgacca
    17641 cgaaggcatt gaccagtacc agaaatgcgc cagccacaga cataaacagc agccaggcaa
    17701 acaagcggga agacgccatc atgcgcgcat ctccgcgcgc tacgccccat agcggataca
    17761 cggacagcag cagcgcaact cctagcgcca gacacagcag tccgttacca atttccggca
    17821 tcatgatgct gggtccttat aaacactcgc cgggcgacgg tgattggctt ccatcgcttt
    17881 ctcaacttct ggcggcgtgt agttttcatc atgtttcgcc agcacttctt tcgcgaggat
    17941 atgattgcct ttttccagtt cgccctgcac cacaacgccc tgcccttcac ggaacagatc
    18001 cggcaaaatg ccttcgtaag agacatctac tgaaccttca gcgtcgtaaa tggtgaaagt
    18061 tacttttagc gaattgggat cgcgctgcac actacccggc atcaccatcc cgccaacgcg
    18121 cagacgctga ccgacttccg gcatttgctg agtttcacgc ttgccgtaga gaatttcccc
    18181 cggcgtataa aagagatcga tattcgagcg cagcgcatat agcaccagac cgatagtcag
    18241 cgccagccct gccaacacgg cacaggcaat ccacaagcgg tttttacggc gaatattcat
    18301 gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac gcgcccgctg ttgcgccacg
    18361 ccacgcagaa ttgcgcgatg ctgcatcacc gagtgcacga ccaaaaccac cagcggaata
    18421 acggtcatca ccaccgccag ccagacaaaa aaggcgtaac cgcccattgc gaaaaattca
    18481 ttccaggaag caaatgcagg ggtcatttac ggcctctttt cagtatcagt tcactcaccc
    18541 acggacggcg tttttccatc agcaaaatca aattacgcat ccgcatcagc gtcagcgtgg
    18601 cagacagcag caggaagcca aaaatcgacc agcgcagcgg cgaacgcatc gccggatcga
    18661 tactttgctg catccgcgtt gatccctgat gcagggtgtt ccaccactcc acggagtaat
    18721 gaataatcgg cagattcacc acgccaatca gcaccagaat acctgccgca cggcccgcca
    18781 gacggcggtc gtcgaaggcg tgccacaggg caatcacacc cacatacaaa aacagcagca
    18841 ccagttcaga agtcagacgt gcatcccata cccaccaggt gccccacatc ggttttcccc
    18901 atgcagagcc ggtaaccagg gcaataaagg taaacacggc accaatgggg gccatcgccg
    18961 ccaccgccag gttggccatt ttcatctgcc agacaaggcc aataaacgcc gccaccgcca
    19021 ttgatgcata aatgcccatc gaccagatcg ccgcaggcac atgcaggtag ataatgcggt
    19081 agctatttcc ctgctgataa tcagccggag caaagccgaa tccccagatc cagccgacgg
    19141 taagcacgac cacactggca attgccagcc acggtataaa ccagccacag atttgataca
    19201 gccgtggtgg gatcgccagt tgatgcagtg ttttccacat agtttcgata ccagactcga
    19261 acaaaaatca gtaatccagc gttattgaat gctgattcgt aacgctgccg ccgtcgcaaa
    19321 aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa attgccagat acccatcaac
    19381 gggcaaatgc atagaagccg cgtccatcgc ggcggtggca aagatgagta atgggatagt
    19441 cagcggtaac accagtatgc tgagcagcac accaccgcgc ttaagtccca ctgtcagcgc
    19501 cacgcccggt gcaccgagaa agccaagcgt aggagttccc agcagcagcg tcagcgccat
    19561 cacttgccag ccataaacat ccattcccag tagcattgct accaggggcg aaaggataag
    19621 taacggcaga ccggttacca tccagtgcgc catcaccttc gccagcacaa cggcgggcaa
    19681 gggtaacggc aacaacatca attgttcaag actgccgtcc tgcaaatcgt cacggaacag
    19741 acgctccagt gccagcaagg atgaaagcag cgcagcaacc cagataatgc ccggcgcaat
    19801 acgcgccagc agttgcggct ccggaccgat actgagcgga aaaagggtaa ttacaatcag
    19861 gaagaaccac agcgggttgg cgatttcggc gctatggcga aacgctacgc gcagctcaag
    19921 acggaaaatg cgccagaaca tcatgcggcc cccgtttgcg tcagtgaaat gcggcgaatt
    19981 ttactttcag caacgttgag cggttggtgg gtagtcagaa tcacaatccc cccctgctcc
    20041 gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt taacatcaat cgcggtaaaa
    20101 ggctcgtcga ggatccataa cgtggcacgg gtcagccaca gacgcgctaa agcgacgcgg
    20161 cgttgttgcc cggcagaaag ttgatttacc ggaatatctt cgaatccggc aagcccggcc
    20221 tgcgccaggg cttccagaca ttgtgcggta tcgccatcgc gatgataaaa gtgcagattt
    20281 tctaacgccg tcagccgggt tttgatcccc ggctgatggc ctatccataa caggttttga
    20341 tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc agagaacctc gcctgcgtca
    20401 gggcgagaca accccgtcag caaacggaga agcgttgtct tccccgcgcc gttgctaccg
    20461 gtgatttgta cccactctcc tgcgttcagc gtaaatgaca agccactaaa taaggttcgt
    20521 tcatcccgct cgcaaagtaa ctctctggct tcaagcatac ccacgcaata accctgttaa
    20581 aaacctggct cgacttcacg catatcgggc agcttgtgcg ctatcccttt atggcaatca
    20641 atacaggttt gcccatcttt caccgcctgg tcatgcatct tcgcggcaac cgatttctgg
    20701 gcggttgtat ccatatactc gaagttgtga cagttacggc actcctgcga gttattgtcc
    20761 ttcatgcgcc gccactcatt ctgtgccatc gtcaggcgat gagcttcaaa tttctgcggc
    20821 gtgtcaataa cgccaaaaat tttaccatac agctctttac ttgctttgag cttgcgtatc
    20881 atcttcggca caaactcgtg cggaacgtga caatccggac aggtcgcacg gacgccgcta
    20941 cggttgttgt agtgcacgga atccatgtat tcctgataca ccgtgttacg catttcgtgg
    21001 cagctaatgc agaactcttc ggtattggct ttttccatcc cggtgttaaa gccgccccag
    21061 aagacgatgc caccgacaaa accgatcaac agcagcgtcc ccagcgccag acggctgggg
    21121 gtacgccacc atttccacag gcgcttaatc agaccaggct tacggtcaga atttcccata
    21181 ataacctctt atttcccgta accttttgac ggggtaaagg tattccccac gattggcgcg
    21241 gtatcagcct gcggcacgtg acattgcaga cagaagtaac gacgtggagc cacttccgcg
    21301 cccactttgc cgtcgctgtc cataaagtga gtaggactga tgcgcggcgc gccagtggtg
    21361 cgatagcttt cgacaccgtg gcactgcaag cagcggttgg tattggtcgt tacctgataa
    21421 ccttcaacgc tatgcgggat cattggcggc tgattcacat agttcagcgg catccgatcc
    21481 tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg atacttccgg cgactggcta
    21541 aaatcgacgc cattagccgc ccaaaccgcg ccgctcacca ccagggccag catcgccgtc
    21601 cattgacaca gcgctttctt caggtcatgg cttttcatga tttcgctccc gaactccatc
    21661 gtgtagttat tgtaaataca tcctcagaac agacatccac gcagcgaccg cacgtcatgc
    21721 aatcgcggct ggtgacctgc accgggcttt gttcatccag caccggggca cgtagcacat
    21781 gcggttccgg gcaaacatga aaacaatcca tacagcggtt acatttctgc cgatcggtgg
    21841 ccgcaacggt aatgacacct ttgctaccca gcacgccata cagcgcgcca accggacaaa
    21901 tgtgcccgca ccagccgtgt tcaacgacca gtagatcaaa taaaaacagc gcgagaataa
    21961 gcagcgcgcc gctgccgaat cccattacca gactgcgccc catcagggaa acggggttga
    22021 tccattccca gatgagtgtg ccggttaagg ccgatcccag cagaatgacc accaacagca
    22081 cgtagcgaat gtggcgaggg atcgtcgcag actgattgag gtcaaagcgc ctgcgtaacc
    22141 agttcgctaa gtcggtaatc gggttcagcg ggcagaccca gctgcaaaat aatcgctttc
    22201 ctgccagggc gtagagcacg gtgataatta ccgcaccggt caacgccacc gtggcaggca
    22261 gatgaccgct ggcaagactt tgcagcgtca tcagcggatc ggtcagcgga acggtgtcga
    22321 ataacaggct actgctgtag ttgccgtgca agatccacac accaaaccac ggaccgctca
    22381 aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa caccagccaa cggtggctgc
    22441 gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc acgtttacga tttgccattg
    22501 ttcccctcca gccagccgaa gcggtaatgg tgacctaact cccctttcgc cagtgacagc
    22561 ggtaacacct tgattgccgg ttgttccagc acgcacactt tttcgcactt accgcagccg
    22621 gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt gcttaccggt acgcgtgttg
    22681 cgctccagct ccagggtgat tgcctcatca attttcgggc attcgcgata acaaacatcg
    22741 cagcgcagcc cctgaaagtt gagacagttt tcctggtcca ccagtaccgc cagccccatc
    22801 cgcgcgtcgt cgatcgattc gatctcacga tccagcgcac cgctcgggca cactttggcg
    22861 cacggaatgt cctcacacat ttcgcaagga atatcccgtg cgacaaaata tggcgtgccc
    22921 gccgacaggc cagaagccag cgtcgccagt tttaaggtgt cgtaagggca agcctgaaca
    22981 cactgaccgc aacgcacaca ggcactggca aaggcgttct cgtttatggc tccgggcggg
    23041 cgcaaccgca cgccagatgc gcgtgcggtt tgctgttgta accccagcgc cacacccacg
    23101 gcagccagcc cgccagctgt gcgaacgaca tcgcgcagaa agcggcggcg accattttga
    23161 ggtttcgctg accgggacat aatggcttac accttctcca gtttgaccgc gcacttcttg
    23221 aagtccgtct ctttcgagag cggatcggtc gcatccagcg tcagtttgtt aaccagctgt
    23281 gcggcgtcga agaacggcat gtacaccagg ccctgcggcg gacggttacg accgcgcgtt
    23341 tcgacaatcg agatcacttc gccacggcga gaaaccactt tcactttgtc gccacggcgc
    23401 agatcgcgcg ctttcgcatc caacgggtga ataaacagga ccgcttccgg gaaggcacgg
    23461 tgcagttccg gtacacggcg agtcatactg ccggtatgcc agtgctccag gacgcgtccg
    23521 gtagagagcc acaggtcgta ctcttcatcc ggtgcctccg ccgccggttc gaacggcagc
    23581 gcgaagatca ccgctttgcc atccggttta ccgtagaact tgtagccttc gcccgctttc
    23641 acgtatgggt cgttaccttc gctgtaacgc cactgcgttt ctttaccgtt cactaccggc
    23701 cagcgcagac cgcgtgcttt gtggtaatca tcgaacggtg ccagatcgtg accgtgaccg
    23761 cgaccaaacc atgcgtactc ttcgaacagc cctttttgca gatagaagcc cagctcgcgg
    23821 gattcatcgt tcagctgatc ttccgccagt tcggataccg ggaatttgct cacttccggg
    23881 gtggcataca gaacttcgta cagcgttttg ccacgcagtt ccggtttctt cgccagcagc
    23941 tcttccggcc atacttcttc agttttgaag cggcgggaga actggactaa ctgccagaga
    24001 tccgatttcg cttcgcccgg tgcctgtacc tgctgacgcc agaactgagt acggcgttcg
    24061 gcgttaccgt aagcgccctc tttctctacc cacattgcgg tcggcagaat caagtccgct
    24121 gccagcgcac tgactgtcgg atacggatcg gagacgatga tgaagttgcg cggatcgcgc
    24181 cagcccggca tacgctcttc attgatgttc ggcccggcct gcatgttgtt ggtacacatg
    24241 gtccagtaaa cattcagctt gccgtctttc agcgcacggt cttgtgctac cgcatgcaga
    24301 ccgattttcg ccggaatggt gccgctcggg atattccact tcttctcgca gatatcacga
    24361 tgtttctcgt tagttaccac catgtccgca ggcagacggt gagcaaaggt gcccacttca
    24421 cgcgcagtac cacacgcgga aggctgcccg gtcagggaga acggaccgca acccggctgg
    24481 gaaattttgc cggtcagcag gtgcaggttg tagaccaggt tgttggccca cacgccacga
    24541 gtatgctggt tgaagcccat cgtccagtag gagatgactt tcttgttcgg atcggcatac
    24601 agctgcgcca gttgttccag ctgatctttt ggtacgccag tcatttcggc ggttttttcc
    24661 agcgtatact cggcaacgaa ggctttgtaa tcttcaaagc tcatcggttc ggaggcgtca
    24721 gaacccggat tcttcgctgc tttttccagc ggatgggtcg gacgtaaacc gtagccgatg
    24781 tccgtcgccc ctttgcgcag gttaacgtgt ttgctaaaga agtcctgatt tatcgcattg
    24841 ttttgaatga tatagttggc gatgtagttc aggatcacca ggtctgattg cggcgtaaag
    24901 atgatgccgt tatccgccag ctcgaagcta cgatgctggt aggtagaaag caccgccacg
    24961 gtgacgttct ggttggagag acgacggtta gtgatgcgcg accagaggat cgggtgcatt
    25021 tccgccatgt tcgcgcccca cagcacaaac gcatcagcct gctcgatgtc gtcatagcag
    25081 cccatcggct catccatacc aaaggtacgc ataaagccaa ctactgccga cgccatacag
    25141 tgacgcgcgt tcgggtcgat gttgttcgaa cggaagcccg ctttgaacag cttggacgcg
    25201 gcataacctt cccagatagt ccactgacca gaaccgaaca taccgatcga ttccggccct
    25261 ttttctttca gggcggtttt gaatttctct tccatcacat cgaaggcctg atcccaggtg
    25321 attggggtaa attcgccttc tttgtcatat ttaccgtttt tcatacgcag aagcggctgc
    25381 gtcaaacggt ctttaccgta catgattttg ggcaggaaat agcccttaat gcagttcaga
    25441 ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca ccacacgccc ctgctgcgtt
    25501 ccgaccagaa cgccgcaacc agtaccgcag aaacggcacg gcgctttatc ccatttaatg
    25561 gcttcctgct gaccaacaac ggcgcgggca acgcccggca cgctgagacc ggcagccgcc
    25621 gcagcggccg caacggcgtt agctttcata aagctacgac gactgagttt catggtgttt
    25681 cctcaccttg ctcttcctgc tggtgataaa ccagcgacac cgccagcacg ccctctacgt
    25741 tgcgtactga ctcaatggtt tgaatcagcg tttcgctgtc ttctgcttcc accaccacaa
    25801 tcaactgacc gctcggcgcg tcgctgacag caacttcaca gccgggaaag gcgttcagtt
    25861 gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac gaccaggctg caaacttgcc
    25921 agttagtgtg catggagata ctccgcagtt atggctgata ccggacagct ggctgcacat
    25981 gctccacatc cgttgcaaag ttggctatta agttgcggct ggtagatccc ggagagcgtc
    26041 gggcgaaaga taattgccat tggctcacag ctatcctgac agcggcggca ttcaactgtt
    26101 tgatacgcca ggcacgcctc cccgatggtg aacagcaaat cccaggccct ggtgtggcgc
    26161 ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt aacagaagct gcactcgtta
    26221 tttttgaaat taacgctcgg atagccaccc gcgccgcgtt gcagaatgtt gttttcacaa
    26281 gcattaatac aggcgtcgca gcgagtgcaa tgggtcagaa aatgagattc atcaccggac
    26341 cagggcggac ggataccgtt actggctttg cgccagcgac cagtgagtat gccccgacgg
    26401 gatgcatcaa tcttcacatt gaccttccat cattaacgcg ctctgaaaat tgagagcgac
    26461 caaataaacc gcataattaa taagccattt ttatagccgc taagatatta aaggatgtgt
    26521 caaagatgca taccccgatc ggggtaaaac ctgtagcagg atcaaaagag tggcgggaag
    26581 cgtggcaaaa acgggctttt gctcacattt caaatggtta taaacatact tatatagcga
    26641 ttaattcacc agagatattt atgtcggttt gttctctcac cagaatttaa cactaaaaga
    26701 gcaggtaaaa ttgtctgaat gttctttaag ttattcataa agcaaattaa taaatctgat
    26761 gaatatgtta accttcagcg acatcatcgg tgaaaaccta taaatgaaga aggaaagcaa
    26821 aaaaatgaag accattctac ctgcagtatt gtttgccgct ttcgccacca cttccgcctg
    26881 ggcggcagaa agcgtccagc cactggaaaa aatcgcgcct tatccacaag ctgaaaaagg
    26941 catgaagcgt caggtgattc agttaacccc gcaagaagat gaatctaccc tgaaagtaga
    27001 actgttaatc ggccagacgc tggaagtcga ttgcaatttg catcgtctcg gcgggaagct
    27061 ggaaagcaaa acgctggaag gctggggcta tgattattac gtctttgata aagttagctc
    27121 gccggtttcg acgatgatgg cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc
    27181 gtatctgggc gatgccggaa tgctgcgtta caacagcaag ctgccgatcg tggtgtatac
    27241 gccagacaat gtggatgtga agtaccgcgt ctggaaggcg gaagagaaaa ttgacaacgc
    27301 ggttgttcgc taaactactg tgaagtgctg caacccgtag gccgaataag gcggtcacgc
    27361 cgcatccgat ccaacgcccg agccggttgc ctgatgcgac gctggcgcgt cttatcagca
    27421 tacgccacat ccggcatacc atgccggatg tggcacatca ttacaacgca atatccgcca
    27481 cttctttttg aacgggttgc ggtttcaact gcggtttcgg cgtactatct gctgcctgcg
    27541 gcttgtcgta tttcagcccc agcacttcgc tggtgtactg caactcgcgt tctgttgccg
    27601 ctacatcacc gttcagcttg cgtccataag acggaacgat cgctttcaac gtagcctgcc
    27661 attgcgggct ggaaacgcga tcgccaaata ctttttccag cagattcaac ataatcggcg
    27721 cggcggttga cgcacctggc gatgccccca gcagcgcggc aatagtccct tgctggtcgc
    27781 tgaccacttc ggtacccaga cgcagtacgc cacctttctc ggcatcacgc ttgataatct
    27841 gtacgcgctg ccccgcttgc cacaaacgcc agtcctcttt tttcgcctgc ggatagtact
    27901 ctttcaacgc ttcaaaacga tcctcttcac tcaacatcac ctgactcacc agatatttca
    27961 ccagatcgaa attatccagc ccgacgtgca tcatcggcat cacgttagag gtggtggtgg
    28021 aactcatcag atcccacaat gaaccgtttt tgaggaattt ggttgagaag gtggcaaatg
    28081 gcccaaacag cacgacgcgt ttaccgtcca gaacacgggt atcgatatgc ggaaccgaca
    28141 tcggtggtgc gccaacggat gctttaccgt aaaccttcgc cagatggtga ttaaccacgt
    28201 ccgggttttc cgaaacaagg aactgtccgc ccaccgggaa accggcgtag tctttcgctt
    28261 ccggaatacc cgatacctgt aacaacttca gcgccgcacc gcccgcgccg ataaagacaa
    28321 atttagcgcg gatgttctgt gcagtgccgt ttttcagatc ggcaacagtg acggtccagg
    28381 tgttatcgtc attacgcttt agggcgcgga cttcgctgct gagttgcagc gaaaaattag
    28441 atttcttctg caaggaagca attaactggc gggtgatctc gccatagttc acatcagtac
    28501 caatttccgt acgcgtggct gccactttct gttgcggatc gcgcccttcc atcactaacg
    28561 gtgcccactc tttgatctgc gcgtgatctt cagagtaacg cataccgcga aacagcgagc
    28621 tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattgac gttatcctcg ccccagacaa
    28681 agctcatatg tggaacggta ttaataaatg aacgtggggt tcgcagcaca ccacgttcga
    28741 cctggtgtgc ccaaaactgg cgggaaatct ggaatgcttc gttaatggcg actgcttttt
    28801 caatactgat gctgccatcg gcgttttgcg gagtgtaatt gagttccatc agtgcagaat
    28861 gcccggttcc ggcgttgttc cagccgttcg aactctcctg cgcgacaccc tccaggcgct
    28921 ccaccatggt catcgaccat tcaggctcca gctcgcgtaa ataggtcccc aacgtggcgc
    28981 tcataatgcc gccgccaatc aacagtacat cagtttcctg ctcctcggac gctttcgctt
    29041 ttgccgccat cgaaacggca ttaagcccca cggccatcga gaagagcatg gcagtcactt
    29101 ttttcatctt gttaatgcct tacttttagt cgctttattg caggtgagat ttgcgcggca
    29161 tcaacggtaa ca
//
LOCUS       DAYPHN010000045        29068 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000045 DAYPHN010000000
VERSION     DAYPHN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.045"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..213)
                     /locus_tag="ACG0BW_001961"
     CDS             complement(<1..213)
                     /locus_tag="ACG0BW_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFK7258928.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYAD"
     gene            complement(216..1568)
                     /gene="wzyE"
                     /locus_tag="ACG0BW_001962"
     CDS             complement(216..1568)
                     /gene="wzyE"
                     /locus_tag="ACG0BW_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFK7258929.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGVALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNTANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(1565..2644)
                     /gene="rffT"
                     /locus_tag="ACG0BW_001963"
                     /gene_synonym="wecF"
     CDS             complement(1565..2644)
                     /gene="rffT"
                     /locus_tag="ACG0BW_001963"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFK7258930.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQRKGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLALFSEENLQILNEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(2641..3891)
                     /gene="wzxE"
                     /locus_tag="ACG0BW_001964"
     CDS             complement(2641..3891)
                     /gene="wzxE"
                     /locus_tag="ACG0BW_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFK7258931.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     FAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(3893..5023)
                     /gene="rffA"
                     /locus_tag="ACG0BW_001965"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(3893..5023)
                     /gene="rffA"
                     /locus_tag="ACG0BW_001965"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFK7258932.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(5028..5702)
                     /gene="rffC"
                     /locus_tag="ACG0BW_001966"
                     /gene_synonym="wecD"
     CDS             complement(5028..5702)
                     /gene="rffC"
                     /locus_tag="ACG0BW_001966"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFK7258933.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKITLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(5680..6561)
                     /gene="rfbA"
                     /locus_tag="ACG0BW_001967"
     CDS             complement(5680..6561)
                     /gene="rfbA"
                     /locus_tag="ACG0BW_001967"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFK7258934.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(6580..7647)
                     /gene="rffG"
                     /locus_tag="ACG0BW_001968"
     CDS             complement(6580..7647)
                     /gene="rffG"
                     /locus_tag="ACG0BW_001968"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFK7258935.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKLLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(7647..8909)
                     /gene="wecC"
                     /locus_tag="ACG0BW_001969"
     CDS             complement(7647..8909)
                     /gene="wecC"
                     /locus_tag="ACG0BW_001969"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFK7258936.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(8906..10036)
                     /gene="wecB"
                     /locus_tag="ACG0BW_001970"
     CDS             complement(8906..10036)
                     /gene="wecB"
                     /locus_tag="ACG0BW_001970"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFK7258937.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(10092..11138)
                     /gene="wzzE"
                     /locus_tag="ACG0BW_001971"
     CDS             complement(10092..11138)
                     /gene="wzzE"
                     /locus_tag="ACG0BW_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFK7258938.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(11150..12253)
                     /gene="wecA"
                     /locus_tag="ACG0BW_001972"
     CDS             complement(11150..12253)
                     /gene="wecA"
                     /locus_tag="ACG0BW_001972"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFK7258939.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(12493..13752)
                     /gene="rho"
                     /locus_tag="ACG0BW_001973"
     CDS             complement(12493..13752)
                     /gene="rho"
                     /locus_tag="ACG0BW_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFK7258940.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(13837..13938)
                     /gene="rhoL"
                     /locus_tag="ACG0BW_001974"
     CDS             complement(13837..13938)
                     /gene="rhoL"
                     /locus_tag="ACG0BW_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFK7258941.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(14079..14408)
                     /gene="trxA"
                     /locus_tag="ACG0BW_001975"
     CDS             complement(14079..14408)
                     /gene="trxA"
                     /locus_tag="ACG0BW_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFK7258942.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            14539..15804
                     /gene="rhlB"
                     /locus_tag="ACG0BW_001976"
     CDS             14539..15804
                     /gene="rhlB"
                     /locus_tag="ACG0BW_001976"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFK7258943.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMSPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGTPRNRRRSG"
     gene            15940..17424
                     /gene="gppA"
                     /locus_tag="ACG0BW_001977"
     CDS             15940..17424
                     /gene="gppA"
                     /locus_tag="ACG0BW_001977"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFK7258944.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVKNEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(17471..19492)
                     /gene="rep"
                     /locus_tag="ACG0BW_001978"
     CDS             complement(17471..19492)
                     /gene="rep"
                     /locus_tag="ACG0BW_001978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFK7258945.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            19579..19860
                     /gene="ppiC"
                     /locus_tag="ACG0BW_001979"
     CDS             19579..19860
                     /gene="ppiC"
                     /locus_tag="ACG0BW_001979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFK7258946.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(19906..21381)
                     /gene="ilvC"
                     /locus_tag="ACG0BW_001980"
     CDS             complement(19906..21381)
                     /gene="ilvC"
                     /locus_tag="ACG0BW_001980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFK7258947.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            21531..22421
                     /gene="ilvY"
                     /locus_tag="ACG0BW_001981"
     CDS             21531..22421
                     /gene="ilvY"
                     /locus_tag="ACG0BW_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFK7258948.1"
                     /translation="MNLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(22418..23962)
                     /gene="ilvA"
                     /locus_tag="ACG0BW_001982"
     CDS             complement(22418..23962)
                     /gene="ilvA"
                     /locus_tag="ACG0BW_001982"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFK7258949.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQNNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(23965..25815)
                     /gene="ilvD"
                     /locus_tag="ACG0BW_001983"
     CDS             complement(23965..25815)
                     /gene="ilvD"
                     /locus_tag="ACG0BW_001983"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFK7258950.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKVC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(25880..26809)
                     /gene="ilvE"
                     /locus_tag="ACG0BW_001984"
     CDS             complement(25880..26809)
                     /gene="ilvE"
                     /locus_tag="ACG0BW_001984"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFK7258951.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(26829..27092)
                     /gene="ilvM"
                     /locus_tag="ACG0BW_001985"
     CDS             complement(26829..27092)
                     /gene="ilvM"
                     /locus_tag="ACG0BW_001985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFK7258952.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(27089..28735)
                     /gene="ilvG"
                     /locus_tag="ACG0BW_001986"
     CDS             complement(27089..28735)
                     /gene="ilvG"
                     /locus_tag="ACG0BW_001986"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFK7258953.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVAGSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVNFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPVTCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPLASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(28738..28788)
                     /gene="ilvX"
                     /locus_tag="ACG0BW_001987"
     CDS             complement(28738..28788)
                     /gene="ilvX"
                     /locus_tag="ACG0BW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFK7258954.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(28875..28973)
                     /gene="ilvL"
                     /locus_tag="ACG0BW_001988"
     CDS             complement(28875..28973)
                     /gene="ilvL"
                     /locus_tag="ACG0BW_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFK7258955.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         6949 a         8038 c         7412 g         6669 t
ORIGIN      
        1 atccgcatat ttaaattcgg cagcgttaat taactccctg acctcggcgt tatcttcaat
       61 ggtcagcatt ttttcggcat taatggcaac cagcgttccc tgcttaagct gcccgtcagc
      121 gaacagataa tcgagggcgt gctgcatatc acgccaaccg atcaactgta agccacgcag
      181 cgtataggtc ggtgccgtgg tgttgttatt cattgttatc cttcaacctg cgtccggagc
      241 gatgattttg tacgtttatg aatgagtccg gcgctttcaa aaagccagta caacagtttt
      301 gcgatcatca gacatgcgcc gaagaccacg ataaaaaaga ccacgcgtga gacaaacgaa
      361 tccagccctt cacgcgccag cacgatcata ttgaagatcg ccccaaagca gaaactgtgc
      421 aatatcgcag ctttatagcg attaggctcg cggttgccca gctcatacag ccagtcgaac
      481 catttgatga tcagtccaac aacaatcgcc ccgagcggga tgaacaacgc gccgcccatc
      541 accaccagtg agcctataag cgtaggcgag atcgccagtc cggagtggtt attcagcact
      601 tcccaggtaa agtagttggc ggtgttcagt accatactcg ggcgacccgg ccacagccag
      661 gaagggataa agacatagaa atcgcggaca atgggagcca gaccctggaa gtcgatgttg
      721 tcgtagttct gcaacagcaa tgccagattc tcccacggcg aaaaggtatc gcgggtgaga
      781 tagagaaacg tatagaacgc ttcatcaccg ctcacattca ttccatagcg ttttagcgcc
      841 agccagaaca tgcccacaat ccccagcacg cccgccgccg ccagcatcca caacgaaatc
      901 cagccgcgaa taatgccaat aaacaggaag atagcgaatg cgatgatgat attggcgcga
      961 gtgccgccga caatcatata agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac
     1021 agccacgctt tgctgtcctg gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag
     1081 taaaagaagc gttttaacgc cacgccggag acttcactgg agaagatctg actgtaggag
     1141 ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct taccagcgcg
     1201 acgcccatca ggatcaccca cgtgagattg gtctccacgc ggttcatggt aaacagcgga
     1261 cggcgcggta catcggcaac gcgtttgcgt aggcgggttt tgtaggtgac gtagtaaacc
     1321 gcgtagaagc agcccgcaga aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca
     1381 acatcaaagc gaaataccag cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa
     1441 aacagcaatg aaaagaagac attgaagtta aaacgcacac ggcgaaactc aaaccaggtc
     1501 agcgtggcaa taaacagcgt gcagagcagc cagacaacaa acaggccact gaattgcagc
     1561 agactcatgc aacctccccg gcggcaatcg ccaacgcccg ctgccagcct tgcagatagt
     1621 tcgggctaaa gaaggcaatg gtgtttttat ccaccgacgc caactggcgc tgcgcttcac
     1681 gcacaatatc ctcgttgaga tcgtcggtag taaacagcac cggcaaatgc tgttccgtca
     1741 tatcctgcca gaacggattt tcccggttaa gcacacaagg aatgcccgcc tgaattagta
     1801 agcacagcgt accaatgccc tgctggcggg caaaaataaa gtaaccaaga tcgcactgac
     1861 gaagtagcgc cagataggcg tcaaattcca gtttttcgtt cagaatttgc agattttctt
     1921 cgctgaataa cgccagcccc gcctgacgaa cttcctcgat gtacgcttcg ttattaggcg
     1981 gatatcccat cggcaccacc acttttaccg tatcgccaaa ttgctgatga acggcgcgca
     2041 aggcagcaat atgctcattg ctgcggtcgc cggagttccc caccagaatg gtcattttcc
     2101 ctttacgttg ccgatcattc gccatcatat tgagtgaagg gtccatccgc gtcgggaagt
     2161 acagcagttc gccccgcacc tttggatgcg ttttggcaaa aaagctcaaa tcgccgcggg
     2221 tggcaaatac acagccgact cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat
     2281 atctcaagcc actggaaagc tcgtacaggt ctgcccccca gatatgccag taaaactggc
     2341 tgggcttaat gccaccactc agcagagcca gccacagtgt gggattgaac tgaccgtgga
     2401 agaaaaaacg ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt
     2461 ttttcccagg gaaaaattgc acagaaagcg ccggacagct atcacttaag ccgtcgtcct
     2521 tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc ggccagcgcg tcgttgaaaa
     2581 accgcagaac ggttcggtta tggtgaggga tatccgatcc cagtacgtga atcagtacag
     2641 tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa agagaaaaat agacgatata
     2701 ggttgccata taggcctgcg ccgcgcccag tgcaccatgc gcagggatta gccagtgggc
     2761 aaataccatc aataaagtga actggctgac ttccgccaga atataaaacc gcagtgacgc
     2821 tttggcgatc accagataac caaagacata agcgcccact tttaacacat cacccactaa
     2881 ctgccaggca aagagatcgc gcatagcggt aaatttattc gacaacagca gccagatagc
     2941 aaaatcacgc agcaaccaga cggtaaaact cgccgccgcc actgccggta agacgaattt
     3001 cagcgattta accacttccc gggtgatatc gcgcttttcc gttagccgcg acaacgtggg
     3061 cagcaaatat acgctgaacg atgccgtaat aaattgcagg taggcatcgg aaatactgct
     3121 caccccttgc cagatcccca cctcatccca gctatactgc gccgccagca gtttacgcat
     3181 catgatgtaa gcaacaggca aggtcaccga cgtaatcaac gccatgagcg taaatttgct
     3241 caactgccct gccaggccgt tatcccagct gggtttcaga tagcttaacg ggattgcacc
     3301 acgcttaatt agcatgatga cggcaggaat caccaccagc gcgggaatca gcgccagacc
     3361 cagcaacgcc ccttcataac cgcccaaacg gtaactgacg tagtacgcaa aaacgccaat
     3421 caagctgccg acaatcaggg ataacgcatt acctgcggca tcgcgaaagc ctttcatcaa
     3481 cgccagtaac aggttgcccc aggcaatccc catttgcacc agcgccacta aacgcaccag
     3541 tccctgatag tcggtattac caaacaatcc ctggctgatt ggcgctgctg ccagcacaaa
     3601 aaccagcgcc attagcgtag agaagccaag taccatcgct gatgaagtgc cgaccacgcg
     3661 gcgcagctgt tggggattat catggtactg agcaacgtat ttggttacgc cattaaagat
     3721 gccagccccg gcaagcacgc cgagcacggt aatcaactgg cggaaatttg ccgccagccc
     3781 aagccccgcc ggaccaaatg acaccgccag caacttaccg accagtaacc cggcaccaat
     3841 cttgaccagt gtactggccg ccgtccacaa ggacgctttt gccaacgaca tatcaggaga
     3901 agtagttcaa caaagtcgca attaccgtac gctgattgac gggcgacagg ttgtagaaca
     3961 gcggcaggcg cagcaggcgc tcactctctt tggtggtgta gcgatcttca ccgtggaact
     4021 caccaaagcg ttcccccgca gggcaaccgt gcagcggaat gtaatggaac accgccatga
     4081 tttccgcttc tttcagaaag ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa
     4141 tgtagaacat atgcgcgttc tgcacgcagc catcgggaat cgacggcagc tcgatacgcc
     4201 cggctttcgc cagaggtgct aacgcatcgt agtagttttg ccacagcgcc agacgttgct
     4261 gattgatacg atctgctgct tccagttgcg cccacaggta cgcagcttgc agatcggaca
     4321 tcaaatagct ggagccgata tcgcgccagg tatatttatc gacctgacca cggaagaact
     4381 ggctgcggtt tgtgcctttt tcacggatga tctcggctcg ttcgatcaac gctttatcgt
     4441 taatcagcgt cgcgccgcct tcaccgcccg ccgtgtagtt tttggtttca tggaagctaa
     4501 agcagccaat atgaccaatg gttcccagtg cacgcccttt gtaagtggac atcacgccct
     4561 gagcagcatc ttccaccaca aacagattat gctttttcgc caacgccata atggtgtcca
     4621 tttcgcaggc cacacccgcg taatggaccg gcacgataac gcgcgttttg tcggtgatcg
     4681 ctgcttcaat caacgtttcg tcgatgttca tggtgtccgg gcgaacatcc acaaaaacga
     4741 tttttgcgcc acgcagcaca aaggcattgg cagtggagac aaaggtgtag ctcggcatga
     4801 tcacttcatc gccaggctgg atatcgagca gcagcgccgc catctccagc gaagcggtgc
     4861 aggacggcgt cagtaacact ttggcgctgc caaaacgttg ctccagccac tgctggcagc
     4921 gacgggtaaa accgccatcg ccacacagtt tgccgctacc cattgccgac tgcatatagt
     4981 cgagttcggt tcccaccacc ggcggtgcgt taaatggaat catgtgatca cctgtataac
     5041 cagtacgcgg tgctttctac attcgcacca ctttgtatgt atcgtttaag cgcggcggtg
     5101 ttgcccatct gggtcgccac ccgcaaagtt attttaccgc gagcatacgc ccagtttagc
     5161 gccgtttgca taagctcagc acctgcaccg cgtccagcca gcaggccaat tcgcgcatct
     5221 gtcgcattga gttcccgtaa agagacataa ccgcgaatat cgccggacgc cgcacgtaaa
     5281 atcagacatt gatgatcaaa ggtgccgcgc acggcatttt caatccactg tgcataaaag
     5341 cgaccgctgg cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg
     5401 ctggctaact gacgtaatgc gggaatatcg gtctcttgtg ccacttctgc gccgctatca
     5461 ccgacattgt tcacgggtag cgccaaatca acttcacctt ctaccaagga gaatcccagc
     5521 tgttgcaggg catccagttc ccccgtattt gatgccgcaa ttttggcctg cacccgtgac
     5581 cacggcgcta aagcgtctga cgtcaggagt gccgcgtcag gactaatgcg cacaatggcg
     5641 ctgttaacac caaagaaggc gttttcccag gttagtggct caatactggc gcggacgggc
     5701 acgaagtaac tccagcagat attggccgta accagttttc gccagcgagc tggcggcgcg
     5761 cttcacgcct tcgtcatcaa gccagccgtt acgccaggcg atctcttcca ggcaggcaat
     5821 cttaaagccc tggcgttttt ccaccgtctg tacaaaggtg ctggcttcaa tcaggctgtc
     5881 gtgagtgcca gtgtccagcc aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc
     5941 cgcctcgagg tacatctggt tgatggaggt aatctccagt tcaccacgct ccgacggctt
     6001 cacctgcttt gcgtactcca cgactttact gtcgtagaaa taaagtccgg tcaccgccca
     6061 gtttgacttc ggctgttttg gcttttcttc cagcgagata gcgcggaaat tgtcgtcaaa
     6121 ctccacgacg ccaaagcgtt ccggatccat cacctgatag ccaaataccg tcgcgccttg
     6181 cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc tgaccgaaga agatgttatc
     6241 acccagcacc agacaagaag gttcaccgtt aaggaaggtt tcaccgatga taaaggcctg
     6301 cgccagaccg tccggactgg gctgttcggc gtattccagc tggataccga actcactacc
     6361 atcgcccagc aggcgctgga aataaccttt atcttccgga gtagtgatga tgagaatttc
     6421 gcggataccg gccagcatca gcaccgacag cggatagtaa atcattggct tatcgtaaat
     6481 cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc aatcgggtgc cggaaccgcc
     6541 cgccaggata atacctttca tgtttgcctc cgctgaaaat tagcctttca gacctaaacg
     6601 ctcgccctga tagctgccgt cctgcacctg cttccaccag ctttcattag ccagatacca
     6661 ctgcaccgtt ttacgcattc cactttcaaa ggtttcctgc ggcagccagc caagttcacg
     6721 ggcaattttc gaagcatcaa tggcataacg cagatcatgc cccggacggt cagcgacaaa
     6781 ggtgatcagg tcacgatagt gcgccacgcc gtgcggcttg ttcggggcaa gttcttccag
     6841 caactcgcaa atggtttcca caacatcgag attcttacgc tcgttgtgac caccaatatt
     6901 ataggtttca ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc
     6961 aacatacagc cagtcacgga tttgctgccc gttgccatat accggcagca gtttacccgc
     7021 cagcgcgttg aggatcatca gcgggatcag tttttccgga aagtggtaag ggccgtagtt
     7081 attcgagcaa ttggtgataa gcgtcggcag accataggtc cgcagccagg cgcgtaccag
     7141 atggtcgctg ctggcttttg aagcggaata agggctgctc ggcgcatacg gcgtggtttc
     7201 ggtgaagaaa tcatccgtcg agtgcaggtc accatatact tcgtcggtag agatatgatg
     7261 aaaacggaac gctgattttt tatcttccgt cagcgcattc cagtaagccc gcgccgcttc
     7321 aagcaatgta taagtcccga caatgttggt ttcaataaac gctgccgggc cgtcaataga
     7381 acggtcaaca tggctttcgg ctgccagatg catgacacag tctggctgat gctcagtgaa
     7441 tacgcgcgcc agttctgccc gatcgcagat atcaactttc tcaaaggcaa agcgctcgct
     7501 ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag gtcagcttat cgaccactac
     7561 caccgcatcg ctcgtttcgt tgatgatata acgcaccagc gccgagccaa taaacccggc
     7621 accacctgtt atcagaatct ttctcatcag cgccagactc ctttggcatc gacgacatac
     7681 tgctgatgga cgttgtcgcc attgataact ttgaactgac tatgatcgac cagcatcacc
     7741 agcacatctg ccgttgccag cgcctcgtca agctgcgcca gagtacaaag cccggtcagt
     7801 ttcttcggca actggtggat gttaggctca acaaccagcg tttcgccgct gtgccactgg
     7861 gcgatcagtt cagcgatttc catcgccggg ctttcgcgca ggtcatcaat attcggttta
     7921 aacgccagac caaagcaggc gattttcagt tcactggcgc gtttatcggt agccgccagg
     7981 caatcagcca ccgccgcttt cacctgatcg ataacccaga aaggtttgtg atcgttcact
     8041 tcgcgcgcgg tacggataag ccgcgcctgc tgtgggttct gtgccacaat aaaccacgga
     8101 tcaacagcga tgcagtggcc gcccacgcca gggccaggct gaagaatatt gacgcgaggg
     8161 tgacgattcg ccaggcgaat cagttcccag acgttaattc cctgatcggc acaaatcagc
     8221 gacaattcat tagcaaaagc gatattcaca tcgcggaagc tgttttcggt gagcttacac
     8281 atttccgccg tccgcgagtt agtgacgaca cactcacctt cgaggaaaat cttgtacagt
     8341 tcgctggcgc gggccgagca aaccggcgtc atgccgccaa tcacgcgatc gtttttaatc
     8401 agctcgacca ttacctgtcc cggcaacacg cgttccgggc agtaggcaat attgacgtca
     8461 gcctgctcgc ccacctgctg tgggaaagtg agatctggac gcatctctgc taaccattct
     8521 gccatcttct cggttgaccc caccggcgag gtggattcga ggatcaccaa cgcgcctttt
     8581 ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc tggctcatga
     8641 tcgcctttaa acggcgtggg tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg
     8701 ctcgctcgta aaaaaccgcc ttctacggca gtttttacta cgctcgccaa atcaggctcg
     8761 acgatatgga tttcgccacg attgatggta tcaaccgcgt gttggttgat atcgacacca
     8821 atcacctgtt tttgccgtga ggcaaacgca gctgctgtag gcagcccgat gtaacccagt
     8881 ccgataacag aaatggtcgc aaaactcata gtgatatccg attatttttt aacgcttcca
     8941 gaatgcgaga gcatgcctga ccatcaccat acgggttatg ggcgcggctc atagcttgat
     9001 attcgttttc gtcttttaaa agacgcgtca cttcctcgac gattcgctgc ttatccgtgc
     9061 cgaccagacg taccgtaccc gccgtcaccg cttccggacg ctcagtggta tcgcgcatta
     9121 ccagcaccgg tttccccagc gaaggcgctt cttcctgaat gccgcctgag tcggtcaaaa
     9181 tcagccaggc gtggttcatc agccagacaa acggtaaata ctcctgggga tcgatcagaa
     9241 tgacattttt cacgtgcccc agaatgcgat tgaccggctc tctgacgttc gggttgagat
     9301 gcaccggata gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc agcgcatggc
     9361 agatttcttc aaagccacga ccgaagctct cacgcctgtg accggtcacc agaatcattt
     9421 ttttattggg gtcgataaac gggtaatttg ccgccagttc tgaacgcagc gtatcgctgc
     9481 tcatcacctg gtcacgcacc cataacagtg catcaataac tgtattaccg gtaatgaaga
     9541 ttcggctgtc agcaacattt tcacgcagca agttttgccg ggaagtttcg gttggagaga
     9601 agtgatacat cgccagatgc ccggtcaatg tacggttagc ctcttccggc cacggcgaat
     9661 agagatcgcc cgtgcgtaaa ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg
     9721 ccgccaggct ggttgccagc gttgtagtag tatcgccgtg aaccagcacg acgtctggtt
     9781 tgaactcggc aagaattggt tttagccctt ccagaatccg acaggttatc tctgtcaggc
     9841 cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac aatggaaaag agtttcagca
     9901 cctgatcgag catctcccga tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat
     9961 cttttgccaa cgcatgcacc aacggagcca tcttgatggc ttccgggcgc gtaccaaata
    10021 cagtcagtac tttcacatcg attctcttcg aataagcggc gagcgccttt gcgctcaccg
    10081 cagcagtgtt gctatttcga gcaacggcgg gttaatgcga caccagcccc gatcagcccc
    10141 ccgacaatgc cccacataat catcaggaag gcacgacgtg ggctatcgcg ttttaccggt
    10201 tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg gatccagggt tggaccaaca
    10261 ttcagggtgt ttaacatggc ccgattctga tcatagtcga gatcaaaggc cggaccgacg
    10321 gcctgtaaat tttccagtcg agcctggagc attggacgcc caagcaggaa catttctgaa
    10381 tcaggcaatt cctcggcagg cacatctgtc gcactgcgcg aaatattatg ctgctcagca
    10441 attttcaacg cctgttcaat actgttcatc cggcggtcgt agatggcttt cgccacctct
    10501 tcctgacgct ttacctgagc tttcatctgg atggtgcgcg ccgcccatgc acctttcagc
    10561 tcatcattca gatggctggc tgcacgctgg ctggcaaaag caacatactg acgtaacagg
    10621 ttattagcgt caggcgcggt ttcggcaatc agcttcacgc tgtcattgac cgcgcgggta
    10681 aagtctccgg ggataaactg gatgttgtta atcatttcat ccagcaacgc cgcatcggct
    10741 ttgctgttgc ccaccatccg ctgcttgtaa tagtcggttt gcagccagaa ctctctgcgg
    10801 gtatcccacg aggccagttg cataacaaac tctttatagg cttcgtccat gaccgatggt
    10861 tggtcggcag aagccatgtt tgaacggaca tccaggttac gcaaaaattg ctgctgtgag
    10921 taatatcccc ccagcatatt caccgttgga cgatcggtaa tcgccgtcga gctccactcc
    10981 tgacgagcaa aaaaagtata cgccagcgcg attaacgcaa acgccagtcc catgccaata
    11041 atccacagct tcccagccca caaggtacga aacaacccac gaatatccag ttcattttca
    11101 gcgtcttcgg ccggtttccc aggcattggt tgtgtcatca catcctcatt tatttggtta
    11161 aattggggct gccaccacga tttctacgca gtctgcgttt tacgcgctta ataaagcgag
    11221 caactttcca ggcacgctta atgcaatatc catagaggaa gaatgctagc aaaaagagca
    11281 ccagcatgac ccactccggg acaaaatgag aatattctgc cagcacccca atggaggcga
    11341 gcagagctgc ggcaagggta atcagcacaa acgcctggcg ggaagtaaac ccggcacgca
    11401 tgatcaaatg gtgaatatgc tggcgatcag gagagaacgg actcatgcct ttacgcaggc
    11461 gacggtacat aatcgccacc atatccatta gcggaatggc gattatccac aaagcggtaa
    11521 ccgggctgat gggatgggtt ttgccctggg tcgtttcgag caggatccag ataacggtaa
    11581 aaccaatcag cgtactgccc gcatcaccca taaagacttt gtagcggcga cccaggatac
    11641 caaggttaag catgatgtat ggcaggatgg cggcgatcat cgcaaaacac cagattgcaa
    11701 ggctggtttg cccgtcgaac cacaaaatca taccgattgc tgcaaacgag acgcaggaca
    11761 acccgcccag caagccatca atgccatcaa ccatgttgaa cgcattaatg gccgcccaga
    11821 cggcaaatag cgtcaggaag taaccaaacg gtccgagcac catctcccag gagccaaaga
    11881 tataacccag gctactgaga taaaggttgc caaacaccat cataacaatg ccaacagcag
    11941 cctgtatggt ggcacggatt tttacgctga tatcaaaacg gtcatccagc gcaccgataa
    12001 acaccagtac accggcacaa gcgagataga gagatgcatg cggaatatag taatcgacaa
    12061 ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatacc cccaacgagt ggtatcaacc
    12121 cctggtgacg tttgcggaag ttaggtttat ccactaaacc gacttttttt gccaccttac
    12181 gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact gatgagatca gtactcactg
    12241 tcagtaaatt cacaatgcat gctctcagag aaaattatta gcagaagtat aaccacgaag
    12301 acctttattc agaagggaaa gtccgttccg aacccaccag tcctgtgcaa aaattaacca
    12361 tttgatatgc aaattgcata acacaccagc aaaatccagg aattttccta acattgccag
    12421 cgcggcatta agattacaga tataaaagca aaacgccacg taaacacgtg gcgtttttgg
    12481 cataagacaa atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat
    12541 tgccagttta ttaatgagga attccattgc atcgatttcg cccatcgggt gaatgatttt
    12601 gcgcaggatc cacattttct gcagttcttc ctgagtagtg agcagctctt ctttacgggt
    12661 accggaacgg ttgtagtcga ttgccgggaa gacgcgtttt tcagcgatct tacgagagag
    12721 gtgcagttcc atgttgcctg tacctttaaa ctcttcgtag ataacttcgt ccatcttgga
    12781 accggtatcg ataagcgcgg tagcgatgat ggtcaggctg ccgccctctt ccacgttacg
    12841 cgccgcaccg aagaagcgtt tcggacgatg cagggcgttg gcatccacac caccggtcag
    12901 cactttacct gacgccggaa caacggtgtt gtaagcgcgc gccagacgag tgatggagtc
    12961 gagcaggatg ataacgtctt tcttgtgctc aaccaggcgt ttcgccttct cgatcaccat
    13021 ttccgcaacc tgaacgtggc gagatgctgg ttcgtcaaag gtagaagcaa caacttcacc
    13081 ttttaccaga cgctgcatct cggttacttc ttccggacgt tcgtcgatca gcagaaccat
    13141 cagcacacaa tccgggtggt tgtaagcaat gctctgagca atgttctgca gcagcatggt
    13201 tttaccggct ttcggcggtg ccacaatcag acctcgctga ccacgaccga taggtgatgc
    13261 cagatccagt acgcgagcgg ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag
    13321 acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc gggcgttttc
    13381 aggtttgtcg aagttaactt cgttaacttt cagcagcgca aaatagcgtt caccttcttt
    13441 cggcgggcga atcttaccag agatggtatc accagtgcgg aggttgaaac ggcggatttg
    13501 gctaggggaa acgtagatgt catcaggacc ggcgaggtag gagctgtctg cggaacggag
    13561 gaaaccaaat ccatcctgca atatctccag tacaccatca ccaaagatat cttcgccact
    13621 ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc ttacgcatac gagccaggtt
    13681 ttccagcccc atattttcgc cgagagtgat cagctcagaa accggcgtat tctttaattc
    13741 ggtaagattc atagtggtgt gagttcttaa acttggggta aatctcgaac ttaatgttgt
    13801 gaatggtatg gcaggatcat ccatgcctgt ttacggtcat cgactcatgt cttttcgctg
    13861 tctggtcaca ggagagtacg cagaactgaa acgacaagac ggattgagtg acgagccaga
    13921 aatctgttca cttcgcattt aagataaaac gggaagcgtt ggaaattaca agattcaaac
    13981 ttaataaggt atgtttaata cgaagtcaac actaagttag catgactcac gccgggcgtc
    14041 cagtttttag cgacggggca cccgaacatg aaattccctt acgccaggtt agcgtcgagg
    14101 aactctttca actgaccttt agacagtgcg cccactttgg ttgccgccac ttcaccgttt
    14161 ttgaacagca gcagagtcgg gataccacgg ataccatatt tcggcgcagt gccagggttt
    14221 tgatcgatgt tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc
    14281 agaatcgggg cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc
    14341 gccccgtccg ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta
    14401 tcgctcatat ataactccac aggaataagc ctggcgtgtt ggtttagttg ttggtgtaac
    14461 attaaccaac taaaggttga ctttatttca ccggatacgc tttcgtaaag caatagtaag
    14521 ctgatattct accacactat gagcaaaaca catttaacag aacagaagtt ttccgacttc
    14581 gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc
    14641 attcaggcac tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa
    14701 accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct tctctctcat
    14761 cccgcgattg ccgatcgcaa ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt
    14821 gaacttgccg tgcagatcca cgccgacgcg gaaccgctgg cggaagctac cggcctgaag
    14881 ctgggtctgg cttacggtgg tgatggctac gacaaacagc tgaaagtgct ggaaagcggc
    14941 gttgacattc tgattggcac cacggggcgt ttaattgact acgccaagca gaaccacatt
    15001 aacctcggtg ccattcaggt agtggtactg gacgaagccg atcgtatgta cgatctgggc
    15061 tttattaaag atatccgctg gctgttccgc cgtatgtcgc ctgcaaacca gcgcctcaac
    15121 atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac
    15181 aatgccgaat atattgaagt ggaaccggaa cagaaaacgg gccaccgtat taaagaagag
    15241 cttttctacc cttctaacga agaaaaaatg cgtttgctgc aaacgctgat cgaagaagaa
    15301 tggccagaca gagcgattat tttcgccaac accaaacacc gttgtgaaga gatctggggc
    15361 cacctggcgg cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa
    15421 cgtctgcgta ttcttgatga atttacccgt ggggatctgg atattctggt tgccaccgac
    15481 gttgccgcgc gtggtctgca tatcccggct gtgacgcacg tctttaacta cgatttaccc
    15541 gatgactgtg aagattacgt tcaccgtatt ggtcgtacag gtcgtgcagg cgcaagtggt
    15601 cattccatca gcctggcgtg tgaagagtat gcattgaatt tgcctgctat tgagacctat
    15661 attggtcact caattccggt aagcaaatac aatccggacg cattgatgac cgatctgcca
    15721 aaaccgctgc gcctcacgcg cccgcgcaca ggcaatggtc cgcgtcgtac tggcactccg
    15781 cgtaatcgtc gtcgttcagg ttaaataaaa aatgtcatac tccaaatcat tcaagctgta
    15841 tcaagatggc aagagaatga atcccgatga gtttactgta gtaagtgatt caggtgaaag
    15901 aatgcagcca acgcagagac agattgaagg atgaagagta tgggttccac ctcgtcgctg
    15961 tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct
    16021 ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc gtctggctgc tggcctgaac
    16081 agcgaaaatg ccctctctaa tgaagcaatg gagcgcggtt ggcaatgttt gcgcctgttt
    16141 gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac ggcgacatta
    16201 cgactagccg tcaatgcggg tgattttatc gccaaagcac aggaaatcct tggctgcccg
    16261 gtacaagtca tcagcggtga agaagaagcc cgcctgattt atcagggggt ggcccacaca
    16321 acaggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac tgaactggta
    16381 accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg
    16441 ctggaacgct attttgccga tcgtaatctg gcgcaggaaa attttgatgc tgcagaaaaa
    16501 gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg ctggaaagtg
    16561 tgcgttggcg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg
    16621 gatgaacgca ttaccctaga aaagttgcag caactgaaac agcgagccat tcattgcggt
    16681 cggctggagg aactggagat tgacgggctg acgctggaac gtgcgttagt gttcccgagt
    16741 ggtctggcaa tcctgatagc catttttacc gaactgaata ttcagtgtat gaccctggca
    16801 ggtggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctgaccgt cgagcaggat
    16861 attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat tgatcaggca
    16921 cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg tgaaaaacga atggcatctt
    16981 gaagcaataa gccgcgattt gctcatcagc gcctgccagc ttcatgaaat cggcctgagc
    17041 gttgacttca aacaagcgcc gcaacacgct gcttatctgg tacgtaatct ggatcttccc
    17101 ggttttaccc ccgcacagaa aaaactgctg gcgacgctac tgctcaacca gacgaatccg
    17161 gtcgatctct catcgctgca tcagcaaaac gccgtaccgc cgcgcgtcgc agaacaactc
    17221 tgccgtttac tacgcctggc catcattttt gccagccgtc gccgtgacga tctcgtgcca
    17281 gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca aggttggcta
    17341 acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca gagctatgtc
    17401 cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga cgcatctttt
    17461 ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc gccagatggc
    17521 tttgcccttt ctgcatccgt tcttcggcgc tcaccacttt gcgctcctgt tcccaaatca
    17581 gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcact agttcgccgt
    17641 actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcga gtaatgccga
    17701 cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc tggtgcggca
    17761 aaaaaccttc ttccataccg accatgtaga cataaggaaa ctccagccct ttcgacgcgt
    17821 ggagagtcat cagttgcacc tgatccagct cttcctcgct ctcgccgcgc tccatcatgt
    17881 cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc agttcgctgc
    17941 cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg cgcatttcgg
    18001 cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg ccatggatta
    18061 gatcacgcac cgcagcaatc ggctcccgct ccgccaggcg ctggatttct gccaaccagt
    18121 gagtgaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc aggcccatat
    18181 caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc agctttttca
    18241 gcgtagccgg gccaatctct cgcttcggcg tgttaacgat acgcagaaat gcgctgtcat
    18301 cgtccgggtt agtcagcacg cgcagataag ccagcaaatc cttgatttca ggacgagaga
    18361 aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg aatttttcaa
    18421 acacccgcga ctgatggttg ccgcgataaa gaatggcgta atctttgtac tgcgttttat
    18481 tgacgaagtg atgcgcgatc agctcgccgg tgacgcgctc ggcttcatgt tcttcgttat
    18541 tcgcgcttaa aacttttagc tccgcgccat agcccagttc ggagaacaga cgcttttcaa
    18601 agacgtgcgg gttattggcg atcaggatat tcgccgcttt cagaatacgt ccggaagagc
    18661 ggtagttctg ctcaagctta atcaccttca gtgccgggaa atcctgactc aacagcacca
    18721 ggttttgcgg acgtgcaccg cgccaggagt agatcgactg gtcgtcatcg ccaactacgg
    18781 taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg gtgttggtgt
    18841 cctgatactc atccaccagc aggtagcgaa ttttgttctg ccagcgttcg cgtacttctt
    18901 cattgcgttg cagcagcaac gtcggcaata aaatcaggtc atcgaagtcg agaacgttac
    18961 aggctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacga tcccgctcgc
    19021 cctttgcctc tgccgccgcc tgcgccggag ttttgagatc gttcttccag ttggagatgg
    19081 tcgaaatcag ctgttgcagg agaactttgt catcttcaat cagcccctcg gtcagctctt
    19141 taagcaaagc aagctgatcg gtatcgtcaa acaacgagaa gtttgctttc atcccaagcg
    19201 ccgcatactc gcgtttgatg atatccagcc ccagcgtgtg gaaggtggag atcatcagcc
    19261 cacgcgcctc tttgcgaccc agcgtctgcc cgacacgctc tttcatctcg cgcgccgcct
    19321 tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata accgcaaccg cggatcagat
    19381 gggcgatttt attggtgata acacgagttt taccggaacc cgcgcccgcc agcactaggc
    19441 aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc ataggaaaat
    19501 cgctcaatga aagtcgggag gggaaaataa cagcgtggta gtatagccag cctcatcctt
    19561 accactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa
    19621 gagaaactgg ctctggatct tctggagcag attaagaacg gtgccgattt cggcaagctg
    19681 gcgaagaaac actccatttg cccatcaggc aaacgcggcg gcgatttagg tgaattccgc
    19741 cagggtcaga tggttccggc gttcgataaa gttgtgttct cttgtccggt actggagccg
    19801 actggcccgc tgcataccca gttcggttac cacatcatta aggtgcttta ccgtaactaa
    19861 cagcaaggcc ttctccggga gaaggccttg aactctgaat cgcacttaac ccgcaactgc
    19921 aatacgtttc atatccgtca tgtagccgcg cagtttctta cctacctgct caatcgcatg
    19981 gctgcgaatc gcttcattca catcacgcag ctgcgcgttg tctaccgcac cttccggaat
    20041 agctttaccc aaatcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca
    20101 agcgtaagag aacagatagt taccgtactc ggcggtatca gagataacta cgttcatttc
    20161 gtacagacgc ttacgggcga tggtgttggc aatcagtgga agctcgtgca gtgattcata
    20221 gtaagcagac tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc
    20281 cgcttttacc atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt
    20341 gccttcatac tgcggcgcgg tttcgaacgc ggttttgccg gtctcttcac gccaggtcag
    20401 cagtttctta tcgtcgttgg cccagtctgc catcatgccg gaggagaatt cgccggagat
    20461 gatatcgtcc atatgtttct ggaacagcgg tgccatgatc tctttcaatt gctcagagag
    20521 cgcgtaagca cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc
    20581 ctgtttcagt gcttcggtga tggtttccca accgaactga atcagttttt ctgcgtatgc
    20641 cggatcggtg ccttcttcca ccagtttgtc gaagcacagc agagagccag cctgcaacat
    20701 accgcacagg atggtttgct cgcccatcag gtcagatttc acttccgcaa cgaaagagga
    20761 ttccagcacg cccgcacgat gaccaccggt tgccgccgcc cacgctttag cgatcgccat
    20821 gccttcgcct ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc
    20881 acgtttgtac tcttcacgca cttcggtgcc agggcatttc ggcgcaacca ttactacggt
    20941 gatgtcttta cggatctgct cgcctacttc cacgatgttg aaaccatgcg agtaacccag
    21001 cgccgcgccg tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc
    21061 cggcgtcagg ttaaccacca gatccgcctg cgggatcagt tcttcgtaag tacccacttt
    21121 aaaaccgttt tcggtcgctt tacgccagga tgcgcgcttt tcggcaatcg cttctttacg
    21181 cagagcgtag gaaatatcca gaccagaatc acgcatgttc aggccctggt tcagaccctg
    21241 tgcgccacag ccgacgatga ctactttttt accctgaagg tagctcgcgc catcggcgaa
    21301 ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc agctgctggc gcagattcag
    21361 tgtattgaag tagttagcca tggtgattcc tcatgatgtt gtgcttctta ttgttcggtt
    21421 cgcgtttgcg agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt
    21481 cacaacgtca cattgcaatt tttgcaacgt caacattgag ggctgcgcca ttgaatttac
    21541 gcgatctgaa aaccttcctg catctggcgg aaagccgcca ttttggccgt agcgcgcggg
    21601 caatgcacgt tagcccgtct acgctttcgc gacaaataca gcgcctggaa gaagatctcg
    21661 gtcagccgct gtttgtgcgt gataaccgca cggtaacgct gacggaagcg ggcgaagaac
    21721 tgcgcgtttt cgcccagcaa acgctgttgc aatatcagca attgcgccac accatcgacc
    21781 agcaagggcc gtcgctctct ggtgaattac atatcttctg ctcggtgacc gctgcctaca
    21841 gccatcttcc accgattctc gaccgcttcc gcgcggaaca tccgtcagtg gagatcaaac
    21901 tcactaccgg tgatgcggcc gatgcgatgg aaaaggtggt cactggcgaa gcggatctgg
    21961 cgattgccgg gaaaccggaa accttgccgg gcgcagtggc gttttcgatg ctggagaatc
    22021 tggcggtggt gctgattgct cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg
    22081 aaaagcccga ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc
    22141 gcattgaact gtggtttcgg cgtaacaaaa tcagcaaccc aatgatttac gccacggttg
    22201 gcgggcatga agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg
    22261 aagtggtgct ggataacagc ccggaaccgg tgcgtaaccg cgtgatgatt ttagagcgca
    22321 gtgatgaaaa aacgccgttt gagctgggcg tgtgtgcgca aaaaaagcgg ctgcatgagc
    22381 cgctgattga ggcattctgg aagattttgc cgaaccacta acccgccaaa aagaacctga
    22441 acgccgggtt attggtttcg tcgtggcaat cgtagcccag ttcattcagc cgggtttcaa
    22501 aatccggttc atgatcgcca agctcgaacg ccgccagtac gcgcccgtag tcggtaccgt
    22561 ggctgcgata atggaacagc gagatgttcc agtgcgtacc cagcgtgttg aggaaacgca
    22621 gcagcgcgcc cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgcg
    22681 atggacgccc gccaaccata tagcgcacat gtagcttcgc catttcgtcg tcggagagat
    22741 caaccacgct gtagccaccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgagac
    22801 cgcggctcag gcgcacgccg acaaaaatgc aggcgttttt ggcatcagca aagcggtagt
    22861 tgaactcggt gaccgagcgc ccaccaagta actggcagaa tttgaggaaa ctgccctttt
    22921 cttccggaat ggtcaccgcc agtaacgctt cacgctgttc gcccagttcg cagcgttccg
    22981 agacgtagcg cagaccgtga aagttcacgt tagcaccgga aagaatatgc gccagccgtt
    23041 caccgcgaat gttgttctga gcgatgtatt ttttcatccc cgccagcgcc agcgcgccgg
    23101 agggttccgc cactgcgcgc acatcttcga acaggtcttt catcgccgca cagatggcat
    23161 cgctatcgac ggtgatgatg tcgtcaagat actcctggca taaacggaag gtttcatcgc
    23221 cgatgcgttt taccgctacg ccttcagcaa acagccccac gcggggcaga tcaacgggat
    23281 gacctgcatc aagtgccgct ttcaggcagg cggaatcttc cgcttccacg gcgatcactt
    23341 tgatttgcgg catcaattgt ttgatcagta ccgcgacgcc cgccgccaga ccgccgccgc
    23401 cgaccggtac aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg
    23461 tgccttgccc ggcgatcacc atcggatggt cgaacggcgg tacccaggtg aaaccctgct
    23521 gctgtgacag ttcgatcgct ttcgctttcg cttcatcgaa atttgcgccg tgaagcagca
    23581 cttcgccgcc aaagccgcgc accgcatcaa ctttgatatc ggcggtggcg gttggcatga
    23641 cgatcagcgc cttcacgcct aaccgtgcgg aagaaaacgc gacaccctgc gcgtggttac
    23701 ctgcagaagc ggtaatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg
    23761 cgtatgcgcc gcgcaactta aagctatgaa ctggctggcg atcttcgcgc ttcaccagaa
    23821 tcacgttatc gagacgcgac gacagttttt ccattttctg tagcggcgtg acctgcgccg
    23881 cttcgtagac cggcgcgcgc agtaccgctc ttaaatattc ggcaccttcc ggggtaccgg
    23941 acaggggttg cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc
    24001 tttgtcggcg ctggttgcca ggctggcgta ggcgcgcaat gcaaaggaga cctgacgttc
    24061 gcgatttttc ggcgtccagg ctttgtcgcc acgagcttcc tgcgcttcac gacgcgccgc
    24121 cagttcggca tcgcttacct gtaactgaat gccacgattc ggaatgtcga tagcgataag
    24181 atcgccgtct tcaatcagac caatgctgcc gccgcttgcc gcttccggcg agacgtggcc
    24241 gatggaaaga ccagaggtgc cgccggagaa gcgtccgtcg gtgatcagcg cgcagacttt
    24301 accgagaccc attgatttca ggaagctggt tgggtagagc atttcctgca tccccggacc
    24361 gcctttcgga ccttcgtacc ggatcaccac cacatcgcca gcgacaactt tgccgccgag
    24421 aatcgcttct accgcgtcat cctggctttc gtacactttc gccggaccgg tgaatttgag
    24481 gatgctgtca tcgacgcccg cggttttaac gatgcagccg ttttctgcga aattaccgta
    24541 gagcaccgca aggccaccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt
    24601 tgcgcgatcg tcatcgagag aatcccaacg gcaatcctgc gagaatgcct gtgtggtacg
    24661 aatgcctgcc gggcctgcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac
    24721 gtcgtattgt tccagcgttt gcggcaacgt caggccaagt acgtttttca catcacggtt
    24781 cagtaacccc gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac
    24841 atcttccata tggtatttct gggtgctcgg cgcaacttta cataactgcg ggactttacg
    24901 cgaaagtttg tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag
    24961 caggtgaagt acggtgttgg tagagccgcc cattgcgata tccaacgtca tggcgttttc
    25021 aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt tcgtcatttt gctcgtagta
    25081 acgtttggtc aattcaacaa tgcgtttacc ggcattgagg aacagctgct tacgatcggc
    25141 gtgagttgcc agcagcgagc cgttgcccgg ctgcgacagg cccagcgctt cggtcaggca
    25201 gttcattgag ttagcggtaa acatcccgga gcaggagccg caggtcggac acgcggaacg
    25261 ttcaacctga tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc
    25321 aaccagatcg agcttgatga tctgatcgga cagtttggtt ttcccggcct ccatcgggcc
    25381 gccggaaaca aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt
    25441 gattttgtcg cagttagaga tgcagaccat ggcgtcggcg cagtgagcgt tgaccatata
    25501 ctcaacggaa tcagcgatca gttcgcgaga tggcagcgaa taaagcatcc ccccgtggcc
    25561 catggcaatc ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc
    25621 ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa
    25681 ttgggtgaac gagttcacaa ccgcgataat cggcttaccg aaatcggcgt cggtcattcc
    25741 ggtggcgcgc cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga
    25801 acggtactta ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt
    25861 gccgtcccat tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg
    25921 tttcgccagt gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac
    25981 ggccttcacc aacctgaata ccgtctacac ttcgcactgg cgtgatttct gccgcagtac
    26041 cggacataaa cacttcatcc gccaggtaca gtgattcgcg cgacagcacc tgctcacgca
    26101 cttcaattcc cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg
    26161 cggaggaggt gaacggtggg gtgaacagca caccgtcttt cacttcaaac aggttttcgc
    26221 ctgcgccttc agagatataa ccattcacat ccaacgcgat accttcctga taaccgtggc
    26281 ggcgtgcttc gctacccacc agcagggaag agaggtagtt accaccggct ttcgccgcgg
    26341 ttgggatggt gtttggcgct gcgcggttcc aggaagaaac catcgcatcg atcccctgct
    26401 ccagcgcttc tgcgcctaga tacgctcccc acgggaaagc ggcgataatc acgtcggttg
    26461 agtatcccgc tggcgggttt acgcccatgc caacatcacc aacgaagatc agcggacgga
    26521 tataggcgct ggtgagattg tttttgcgga tcacgtcacg acaagcttcc atcagctcat
    26581 caatgctctg cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat
    26641 gctcacgatg gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa
    26701 aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc
    26761 gaaccatctc cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc
    26821 ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt
    26881 gcgacgtcca ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca
    26941 acggtcaatt cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag
    27001 acgtggaaac cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag
    27061 cgagccgata cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac
    27121 tggcaccagg cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca
    27181 ggtatggccc atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg
    27241 tgatgtgttg gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcgg
    27301 taagggtggt ttcgctgtat cgttcctgga aaaacagttg ctgccattgt cgaaccatcc
    27361 ctaaccgttg gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc
    27421 ccagctcttg cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt
    27481 tcggtcgcgc gacttgtgcg ccaaccgccg caggtaaacc aaaacccatg gtgcctaagc
    27541 cgctggaggt gatgaaattt tccgggcgag tgtgggcgat gtgctgggca gcccacatct
    27601 ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt
    27661 ttaacaacaa cggcgcgtag atagcgtcac cgggatggtc gtaacgccag gtatgttcat
    27721 cacgcagctg cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaatgctg
    27781 gtaacagagc atttaaatca ccctgtaatg ccacatgtgc ctgacgcagc ttgttcattt
    27841 ctgccgggtc gatatccata tggataacac tggcgagtgg tgcgaaggtg ttcagtttgc
    27901 cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgaaccg
    27961 cgaagttcgc cgctttggtg ccgtgcattc ccagcatgcc cagatagtac ggataatctg
    28021 cttcaactgc gcccagccct ttcagcgtgc aggtgacagg catttttgta gcagcgagaa
    28081 attcacgcag agcgggaact gcctgcgcca tgcccacgcc accgccaacg tacagcatcg
    28141 gtttttgcgc ctttgccagc atctggcgcg cttgctcgac ttcggcatgt gggaaattca
    28201 cttcgttttc aacggtggtg aaccatggtt ccagatcgcc gctggctaac tggatatctt
    28261 ttgggatatc gaccagaacc ggaccaggac gacctgagcc ggcaacgtcg aatgcttcag
    28321 ccatgatgcg cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta
    28381 atgacaatcc cagtacatcc acttcctgaa atgcgtcagt gccgataaac ggtgcggata
    28441 cctgaccggt gatagcaaca acggggatgg aatctaacag cgcgtccgca agcccggtta
    28501 tcaggttggt tgcacctgga ccagacgtgg cgatacatac gccagttttg ccggtagcac
    28561 gggcataacc gatagccgcc attgctgcac cttgctcgtg tcggcacagc aagtgttcca
    28621 cgccaccgtc atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa
    28681 cggtgttcac accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta
    28741 gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc
    28801 agttatgttt ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg
    28861 ttaaggcttg atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata
    28921 atcaccacca cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct
    28981 tttcttgcat cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac
    29041 aagatatttt ttggccgatt tttatgat
//
LOCUS       DAYPHN010000046        28772 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000046 DAYPHN010000000
VERSION     DAYPHN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.046"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            124..1479
                     /locus_tag="ACG0BW_001989"
     CDS             124..1479
                     /locus_tag="ACG0BW_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFK7258956.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     ARLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            1588..22365
                     /locus_tag="ACG0BW_001990"
                     /pseudo
     CDS             1588..22365
                     /locus_tag="ACG0BW_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            22369..24531
                     /locus_tag="ACG0BW_001991"
     CDS             22369..24531
                     /locus_tag="ACG0BW_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFK7258957.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSTDSLNPWRLPLIAVFDNQQIGVITRRDNHDNISIQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAHLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQSEPTARRVVLQEKNKGSAA"
     gene            24528..25703
                     /locus_tag="ACG0BW_001992"
     CDS             24528..25703
                     /locus_tag="ACG0BW_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFK7258958.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25700..26107
                     /gene="cueR"
                     /locus_tag="ACG0BW_001993"
     CDS             25700..26107
                     /gene="cueR"
                     /locus_tag="ACG0BW_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFK7258959.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(26111..26314)
                     /locus_tag="ACG0BW_001994"
     CDS             complement(26111..26314)
                     /locus_tag="ACG0BW_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258960.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIN"
     gene            complement(26311..26733)
                     /locus_tag="ACG0BW_001995"
     CDS             complement(26311..26733)
                     /locus_tag="ACG0BW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258961.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKSIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(26787..27836)
                     /locus_tag="ACG0BW_001996"
     CDS             complement(26787..27836)
                     /locus_tag="ACG0BW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFK7258962.1"
                     /translation="MKADDAQHAIMVAQTDIDANKAAIADTRNDVSAVQSDVTNIKGD
                     VAHAQSTADHANANANTALMNGVKLSGAVTENKNNIEQNRSDIADQQQQLDETRKTVA
                     ATGDVQTAARYQSMIDARQTAAANAQQAQADTQQQQMDDQQKQIDATQKTVSALGDAQ
                     TNAHYQEMVNAGLRAQNDANARTAAEQKQKIDTLATNQATQQHIDSVQYGEQIQRLAQ
                     DSTQTHEQIDSLTQDVTQTHQQLSNTQKRVADNSQQITTLNNHFDSLKNEVEDNRKEA
                     NAGTASAIAIASQPQVKTGDLMMVSAGAGTYNGESAVSVGTSFNAGTHTVLKAGISAD
                     TQSDFGAGVGVGYSF"
BASE COUNT         6561 a         8331 c         8422 g         5458 t
ORIGIN      
        1 tgcatttctt tgccaatatc aatggtcgac aaaagagatg cacaagttat tacccttctt
       61 aataaaatga gactggttat aaaaaccagt taaaataatg acctggaagg aagtgactta
      121 tctatggcgc gattccagtt taaaagccgt aaaaataatg ggtttatttt tttaataagt
      181 tttatggtga tgggagaagc tacaattgct gcaccgctgc cacaatgggc gaacgcccca
      241 gcggtaacgc cagttgcccg attatcttta caggaaagta tattacgcgc ctttgcgcga
      301 aatcccgacg tcactcaaca ggcggcgcag ataggcattg gcgaagcgca aattgatgaa
      361 gccaaaagtg cctggtatcc gcacgttgga ttaaccggca acgcggggcc gtcccgacaa
      421 acggactcca gcggcaggct tgataacaac gtttcgtatg gcataaccct gacacagctg
      481 gtgtatgact ttggtaaaac aaacaacgat atcaatctgc aaactgccgc ccgtgacagc
      541 taccgcttta aattgatggc aaccttaacc gatgttgcag agaaaacggc gactgcctat
      601 atggaagtca gtcgttatca ggctttgtgc gatgcggctc aacgcaatat tcactcgctg
      661 gaaaacgtct acaacatggc ggcattgcgc gctaacgctg gcctgaactc atcgtcggat
      721 gaattacaag cccagacgcg tattgccgga atgcgctcaa cgctggagca atatcaggcg
      781 cagatggcga gcgccaaagc gcaactggcg gtgctcactg gcgtacagcc ggaggcgata
      841 gccgcgccac ctgctgaatt agccgagcag ccggtgtcgc tgaaaaatat tgattaccag
      901 tccatcccgc tggtgctggc ggcagaaaac ttacgccagt cagcacagta cggcgtggaa
      961 aaaacgaaag cgcaatactg gccaacgctg agtattcagg ggggcaaaac gcgctaccag
     1021 accagcgacc gctcgtattg ggatgatcag ctacaactga acgttaacgc gccgctgtat
     1081 cagggcggcg cggtttctgc ccaggtgcaa caggccgaag ggcaacaaaa aatctctgcc
     1141 tcgcaggtcg aacaggcaaa actggatgtg ctgcaacgag cgtctgtggc atatgccaac
     1201 tggacaggcg cacggggtcg cgaagaagcc ggtttagcac aatccgaaag tgcgcacaaa
     1261 acgcgagatg tgtaccaaaa tgaatataaa ttgggcaaac gcagtctgaa tgatctatta
     1321 accgtcgagc aagatgtctt tcaggcgcaa tcggctgaaa taaatgccaa ttatgatggc
     1381 tgggtcgccg ccgtaaatta tgccgctgcg gtgaataacc ttattccatt agcgggaatt
     1441 aaacagggct tatacaacga cttacccgac ttgaaataat cctgccatta ttaatatcag
     1501 gtatgcctcg ttgttaagtg attacctttg tcttatttag ataaatggac tcgctaagga
     1561 tgtgcgccat ttatcaggaa tgccattatg agcctgatta ttgatgttat ttcgcgtaaa
     1621 acatccgtca aacaaacgct gattaatcct ggcgacgtca cggttgttat ttatgagcct
     1681 tccgtggtgc aggttcatgc tcaggcctct gccgttgcgc gttacgtccg tgaaggaaat
     1741 gacctgctga tctatatgca ggacggcacg gtgatccgct gcaacggtta tttcctgcaa
     1801 gcggcgaata ccgctgaaca atcggaactg gtgtttgccg atggacaaca gctcacccac
     1861 gtcacctttg ccgatactgc tgcgggtgga ttagcccccg tagaactgac tgcccagacc
     1921 accgcgattg aaagcattgc gccatttctt gataccgttg ctcagaccag caccttcccg
     1981 tggggatggc tggcgggggc ggcggtaggc ggtggcgcgc ttggtgcact gctggcaagc
     2041 ggtggcgatg gcgactcgaa aacggaagtg attaataacc ctacgccacc cgctgagcct
     2101 ggcaacgcca ctccatcatt tttagttacc gataatcagg gcgatcagcg cggcattctc
     2161 gccaccaatg acatcaccga tgataccacg ccaaccttta gcggcagcgg gcaggcgggg
     2221 gcgactattc agattaaaga cagtaacggc aatactattg ccagtactca ggtagacaac
     2281 aacggtcact ggagtgtctc gttacccacg caaagtgcag gtgaacatac ctggtcagtg
     2341 gtgcaaattg tcggcagtac catcactgac gccggttcga taacgttaac catcgacaat
     2401 agtcaggcca gcgtgcaggt tgccaccacc gcaggcgata acattattaa cgccagcgaa
     2461 caggccgccg ggtttacgct ttctggtacc agtagccatc tggcgcaggg aacagaactc
     2521 accgttacgc taaacggcaa aacctacacc accagcgtag gcgctaacgg tgcctggagc
     2581 gtgcaggtgc cgaccgccga tgcacaggcg ttaggcgaag ggaatcaggc agtgctggtc
     2641 agcgggaaag acgccacagg caatacggtc accggcgcgc agcttttaac ggtcgatacc
     2701 caaccgccaa cgcttgccat caacactatc gctcaggaca acatcgtcag tgctgcggaa
     2761 cataacgccg cgctggtact gagcggcacg tcgaatgcag aagcggggca aaccgtaacc
     2821 ctgaccgtca acgggaaaag ccatacagca accgtcggta gcgacggaac ctggcaagtg
     2881 acgctgcctg ccacggaggt ccaggcactg gcggagggga actacgctgt caatgccagt
     2941 gtcagcgatc gggcaggaaa caccaccagc aacagcgcga atttcacggt agacacctca
     3001 gcacccgtgg tcagcgttaa caccgtggcg ggcgacgata ttcttaataa tgccgagcag
     3061 gccgtcgcgc agatcatctc cggacaagtc agcggtgctt ctccaggcga tacggtaacg
     3121 gtgaaattgg gcactcatgt cctgacgggc atcgtgctgg cggatggcag ctggaatgtg
     3181 gcgctggacc cggcggtaac ccgcacgctg gatcgcggag ccaatacgat tttcgtcacc
     3241 gtaacagatg ctgcaggaaa tactggcgcg gcgtctcgag caatcacgct ggtcggtgtt
     3301 tctccgttga tcaccattaa caccgtctct ggcgatgaca ttatcagtgg cgcagaaaaa
     3361 ggtgcgccac tgacccttac cggtagcact caacaggccg agacaggaca aaccgtcaca
     3421 gtaaccctgg ctggacagag ttttaccact accgtgcagg ccgatggctc ctggagtctg
     3481 acggtacctg ccgccgcgat gggaaatctg cctgacggcg cggtggcgat taccgcttct
     3541 gtgacggatc tcagcggcaa taccggcaac acctcccgca ccattaccgt cgacagccag
     3601 gccccggcgt tgagcatcga ctcgctgact gccgataaca tcatcaacgc cgccgaaagt
     3661 gggcaggatc tgcccatcac cggcaccacc gacgctcagc cagggcagac ggtgaccgtc
     3721 acgctgaacg ggcagaccta tcagggcgtc gtgcagtccg acggcacctg gagcgtgacc
     3781 gttcccgccg ccaatgttga cgcactggct gacggcaacg ccacggtcac cgccagcgtg
     3841 aacgatgtcg ccggtaaccc gaccagcgtt tcacgcgtgg cgctggtgga tgccacgcct
     3901 ccggtggtga ccattaatcc ggtggcgacc gataacgtca tcaacacgcc ggaacacacc
     3961 caggcgcaaa tcatcagtgg cacggttact ggcgcgcagg cgggcgatat tgtcaccgtt
     4021 acgttaaata attccgatta caccacggtg gtggatggtt ccggcaactg gagtctgggt
     4081 gttccggctt ccgttgtcag cggactggtg gatggcagtt atcctgtcag cgtctcggta
     4141 accgacaaag ccggaaacac gggcagccag tcgctaaccg tcacggtcga taccgccgct
     4201 ccgatgattg gtattaacac tattgcgggc gatgatgtga ttaacgccag cgaaaaaggg
     4261 gccgatgttc aaattaccgg caccagcgat cagcctgtta ataccgccat caccgtaacg
     4321 ctgaacgggc aaaattacac caccacgacc gacgcctccg gcaactggag cgtgacggtt
     4381 ccggcattgg cggtaaccgc actgggtcag gccaactaca cggtaacagc ggcggtgacc
     4441 aatagtatcg gcaacagcaa caccgccagc cataacgtgc tggtcgacag cgcgctgccc
     4501 ggcgtgacca ttaacctggt ggcaaccgac gatattatta acgccgccga agcgggcgtt
     4561 gcgcaaacca ttagcgggca ggttactggc gcggaagatg gcgacacgat aactattacg
     4621 ttgggcggta atacttatac ggcgacggtg ggcagcaatc tcacctggag cgtgagcgtt
     4681 cctgcggcag atattcaggc gctgggcaat ggcgatttaa cggtgagcgc ctcggtcacc
     4741 aatcaaaacg gcaacaccgg cagcggcacg cgggatatca ccatcgacgc caatctaccc
     4801 ggtctgcggg tcgatacggt ggcgggcgat gatgtggtca atattattga gcatgggcag
     4861 gcgctggtga ttaccggcag cagctcgggg ctggctgaag gcacgccgct taccgttacg
     4921 attaacaatg tggaatacat cactgcggtg caggccgatg gtagctggag cgtgggcgtc
     4981 acggcggcgc aggttagcgc ctggcctgcg gggacggtta atattgccgt atccggggaa
     5041 agtagcgccg aaaatccggt gagcattacg catccggtta tggtcgatct cacgccggcg
     5101 gcgattacca tcaacaccat cgccacggac gacgtgatta acgccgcaga aaaaggcgct
     5161 gatttaacgc tttccggcac caccactaac gtagaacccg gtcaaaccgt caccgtcacc
     5221 tttggcggga aaaattacac tgccagcgta gcgagcgatg gtagctggac tgccaccgta
     5281 cccgccgccg atctggcgtc attacccgag ggcagcgcct ccgcacaggc cagcgtcagc
     5341 aatatcaacg gcaatagcgc ctcggcggtg cataactaca gcatcgatag cagtgccccg
     5401 accatcatta tcaataccgt cgccagcgac aatatcgtca acgccagcga agccgatgcg
     5461 ggtgtgacgg tgagcggcag caccaccgct gaagcggggc agattgttac ggttacgctc
     5521 aatagcccga ccgtgcagac ctatcaggca acggtgcagg cggacggcag ctggagcatc
     5581 aatattccgg cggcagatct tgaggcattg accgatggca gccacaccct gaccgccacg
     5641 gtcaatgaca aagcgggcaa cccggcgagc accacgcata atctggcggt ggatctcacc
     5701 gttccggtac ttaccatcaa caccattgcg ggcgacgata ttattaacgc cgccgaacac
     5761 gggcaggcgc tggtgatttc cggttccagc actggcggag aagcggggga tgtcgtcacc
     5821 gtcacgctaa acagtaaaac ctacaccacg acgctggatg cttccggcaa ctggagcgtc
     5881 ggcgttccgg cggcggatgt cacggcactg ggcagcggcc cgcaaactgt taccgccacg
     5941 gtgactgatg cggcgggtaa tagcgataac gagacgcaca cggttaccgt taaccttgct
     6001 gcgccgacca ttggcattaa caccattgct accgatgacg tgattaacgc caccgaaaaa
     6061 ggcgcggatc tgcaaatttc cggcaccagt aatcagccag caggcaccac aattacgctg
     6121 acgctgaacg ggcaaaatta cactgcgacg acggacgccg caggcaactg gagcaccacg
     6181 gtgccagctt ccgcagtcgg tgcgctgggc gaagccagct acacggtgac ggcgaacgtc
     6241 accgacagcg cgggcaacag caattccgcc agtcataacg tgcaggtcaa taccgcgctg
     6301 cctggcgtca cccttaatcc ggtggcgagc gacgacatta ttaacgctgc cgaatcgggc
     6361 gtggcgcaga ccatcagcgg gcaggtgacg ggcgcggcgg cgggcgatac ggttaccgta
     6421 acgcttggcg ggaaaactta caccgctacc gtgcagggga atttaagctg gagcgtggac
     6481 gttccggcgg cggatattca ggccatcggc aatggcgacc tgacagttaa tgcctcggtg
     6541 accaacggcg ttggtaatac cggcagcggt tcgcgagata ttactatcga cgctaatttg
     6601 cctggcctgc gcgtcgatac cgtggcgggc gatgatgtgg tcaacagcat cgagcacagt
     6661 caggcactgg tgataactgg tagcagcagc gggctggcgg cgggcgcggc gctgacggtg
     6721 gtgattaaca cggtcactta cgctgcgaca gtattagccg atggcacatg gagccttggc
     6781 gttccggcgg cagatgtggg caactggcct gcgggtacgg tgaatatcac ggtttcaggt
     6841 gccactagcg caggaaaccc ggtcactatc acccatccgg tcaccgtcga tctggcggcg
     6901 gtggcgatct ccattaacac cgtttccggc gacgatgtga ttaacgccgc cgaaaaaggg
     6961 gcagatttaa ccctttccgg cagcacctcc ggcgtggagc tggggcaaac ggtcaccgtt
     7021 acctttggcg gcaaaaccta caccgccacc gtagcgggcg atggcagctg gacaaccagt
     7081 gtacctgctg ccgatctcgc cgcattacgc gacggcgacg ccacggtaca ggccagcgtc
     7141 agcaatatta acggcaacac ggcttcggcg acccacgcct acagcgtcga tgccacggca
     7201 ccgacgtttg ccattaacac catcgccacc gacgatattc tgaacgccgc cgaggcgggc
     7261 aatccgctaa ccatcagcgg cagtagcacc gccgaagcgg ggcagacggt aaccgtcacg
     7321 cttaatggcg tgacttacac cggcaccgtc caggcggatg gcagctggag cgtcagcgtg
     7381 ccgacggcgg atctcagcaa tctgaccgcc agccagtaca cggtcagcgc ctcggtaagc
     7441 gataaagcgg gtaacccggc aaccgccact catgggctgg cggtggatct caccgttccc
     7501 gtattaacca tcaacaccgt ctccggcgat gacattatta acgctgccga acacggacag
     7561 gcgctggtga tctccggctc cagcactggc ggggaagcgg gcgatgtcat caccgtcacg
     7621 ttaaacagta aaacctacac caccacgctg gacgcttccg gcaactggag cgtcggcgtt
     7681 ccgctgtcag acgttactgc ccttggcagc ggcccgcaaa ccatcacagc taccattacc
     7741 gacgcggcag gcaacagcga tgatgccagc cgcacggtca ccgtgaatct gaccgcgcca
     7801 accattggca ttaatactat tgccgacgat gacgtgatta acgctacgga aaaaggcgcg
     7861 gatctgcaaa ttaccggcac cagtaatcag cccgcaggca ccacaataac ggtgacgctg
     7921 aacgggcaaa attacaccgc cataacagat gcttccggca actggagcgc cacggtgcca
     7981 gcgtcagcgg tcagtgcatt gggtgaggcc aactacacgg taaccgcgaa cgtcaccgac
     8041 agcgcaggca acagcaattc cgccagccat aacgtgctgg tcaacagcgc gttacctgcc
     8101 gtcaccatta acgcggtggc ggccgatgac attattaacg ctgccgaagc gggcaatgcg
     8161 caaaccatca gtgggcaggt gacgggtgcc gcggcgggcg acacagttac cgttacgctg
     8221 ggtggcaaca cctacaccgc cacggtgcag gctaatttaa gctggagcgt tagcgtgccg
     8281 gcggcggata ttcaggccat cggcaatggt aatctcacag taaacgcctc ggtcactaat
     8341 ggcgtgggca acaccggcag cggctcgcgc gatatcacga tcgatgccaa tctgcctggc
     8401 ttgcgggtcg ataccgtggc gggcgatgat gttattaaca gcattgagca caatcaggcg
     8461 ctggtgataa caggtagcag caccgggtta acggcgggaa cggcgttaac ggtcgagatt
     8521 aacaacgtta cttatggcgc gacggtatta gccgacggca cgtggagcct cggtattccg
     8581 gcggcagacg tcagcaactg gcctgcgggt acggtggata ttacggtaag cggcaccaac
     8641 agcgccggaa caacctctac cattacccat ccggttaccg tcgatctggc ggcggtcgcc
     8701 atcaccatta acaccctttc cggcgatgat gtgatcaacg ccgtggagaa aggcgaaacg
     8761 ctggtcgtaa gcggcagcac cagcggtgtg gaagctgggc agacggtgac cgtcaccttt
     8821 ggcggtaaaa attacaccac cactgtggaa gccaacggca gctggacggt gaatgtgccg
     8881 cctgccgatc tcgctgcgct accggacggc gcgggcaacg tgcaggcgag cgtcagcaat
     8941 attaacggca acagcgccca ggccgatcgc gcgtatagcg tcgatgccac cgcgccgctt
     9001 attactatca acaccatcgc cagcgacgat actcttaacg tgagcgaagc gggcgcgggg
     9061 atcaccatca gcggcaccac cacggcgcag gccgggcaga cgctgaccgt cacgctcaat
     9121 aacaacacgt accagaccac cattctcgcg gatggcacct ggagcgtgaa cgttccggca
     9181 acggatttaa gcggattaac cgccagcagt tataccgtga ctgcgacggt gagcgacaaa
     9241 gcgagcaacc cggcaagcgc cgatcatgcg ctggcggtgg acgttaccgc tccggatctc
     9301 accattaaca ccgtcgcggg cgatgacatc atcaacgcta tcgaacacgg gcaggcgctg
     9361 gtggtgagcg gcaccagcac gggcgcggcg gcaggcgatg tggtgaccgt ctcgctcaac
     9421 ggcaaaaact acaccaccac gctggatgcc tctggtaact agagcgtcgg tatcccggcg
     9481 gcagatgtga cggcgctggc aaccggaagc cagaccatta ctgccagcct gagcgatcgc
     9541 gcgggcaaca gcgacagcgc tacccataat gtcaccgttg atcttagcgg cccgacgctg
     9601 accatcagta ccgtctccgg cgacgacatc atcaataatg cggagaaaac tcaggatctg
     9661 accatcagcg gcggctccag cggtctggcg accggcacca cggtcaccgt gatgttgaac
     9721 ggcctggcgt acagcgccac caccgatagt tccggcaact ggagcgttac cgtgcctgct
     9781 tcggcggtcg gtgccctggg cgaggcggtc tattcgatta gcgccagcgc caccgacagc
     9841 gcgggcaaca gcggcagtac cacccatacg gtgaatgtcg aatcgctcct gcctggggtg
     9901 atcattaata ccgtggcggg cgatgacatt atcaacgccg ctgaaatcgt tgtggcgcag
     9961 accatcagcg gtcaggtcac gggaacggcg gttgccggga atacggtgat tgtcaccatt
    10021 ggcggcaatc aatataacgc caccgtgcag tcagatttaa gctggagcgt cagcgtaccg
    10081 gcgaacgttt tgcaggcgct gggtaacggt gaactgacca tcagcgcctc ggtgaccaat
    10141 tccgcaaata ataccagcac cgcgacgcac gatatcgtga tagatgccaa cctgccaggt
    10201 ctgcgcgtcg ataccgtggc aggcgatgat gtgattaaca gcatcgagca cactcaggcg
    10261 ttggtgatca ccggtagcag tagcggactg gcggcgggcg cggcgttgac ggtggttatc
    10321 aatagcgtca cctacggcgc aacggtactc gccgatggca gctggagcgt cggtgttccg
    10381 gcggcagatg tcacaaactg gcctgcggga acggtcaata ttgccgtttc tggcaccaat
    10441 accgccggaa ccacaaccag cattagccat ccggtcacgg tcgatctcgc ggccgtggcg
    10501 attaccatta acaccctttc cactgacgat gtgattaatg ccgccgaaaa aggcagcgat
    10561 ttgcagctct ccggcaccac ctccgatgtg gaagcggggc aaaccatcac cgttatcttc
    10621 ggcggcaaaa gctacaccac cacggttgcc gcgggcggca cctgggggct gacaatccca
    10681 gcagccgatc tcgcaacctt gcctgacggc gcagcaaacg tccaggccag cgtcagcaac
    10741 gtcgcaggta acagcgccca ggcaacgcat gcttacagcg tcgatgccac cgcgccttcc
    10801 gttaccatca acaccatcgc cacggacgat attcttaacg ccgacgaagc gggatcggcg
    10861 ctgaccatca gcggcaccag cacggcggaa gccgggcaga cggtgaccgt gacgctaaac
    10921 ggcgtcaatt acagcggcaa cgtgcaggca gacgggagct ggagcgtcag cgtcccgacc
    10981 ggcgatttag ctaacctcac tgccagcccc tatacggtga gcgcagcggt cagcgacaaa
    11041 gccggaaatc cggcctcggc gacgcataac ctgacggtgg atcttgttgc gccggtcgtt
    11101 accattaaca cggtagcggg cgatgacatc attaacgcca cagaacacgg acaggcgcag
    11161 attatcagcg gctcggcaac gggcgcgacg accggtaaca cggtgtccgt gacgattggc
    11221 acgaccactt acaccaccgt gctggacgcc aacggcaact ggagcatcgg cgtgcctgcc
    11281 agcgtgattt ccgcactggc gcaggggaat gtgaccatta ccgccacggt caccgactcc
    11341 gcaggcaaca gcggcacggc ctcgcacact gtcagcgtgg cgctcggcgc gccgatactg
    11401 agtattaaca ccatcgccgt tgatgacatc attaatgcca cggagaaagg cgcggatctg
    11461 gcgatttccg gaagcagcaa ccagcctgcg ggtacgcaga taaccgttac gctcaacggg
    11521 caaaattaca ccaccactgc cgatgcatcc ggtaactgga gcgtgaccgt tccggcgtca
    11581 cgggtgagcg ccctcggtga agccacttac acggtgactg ccgccgccac tgacgccgat
    11641 ggcaacagcg gttccgccag ccataacgtg caggttaata ccgcgctgcc gggcgtcacc
    11701 attaacgttg tcgcgagcga cgatattatt aacgctgccg aagcgggcgc gggccagagc
    11761 atcagcgggc aggtgacggg cgcggcagcg ggcgacacgg taaccgtcac gctcggcgag
    11821 gcgacttaca ctgccacggt gcaggcgaat ttaagctgga gcgtcgatgt tccggcctcc
    11881 gcgctacagg cgttgggcaa cggtgaactg accatttcgg cttcggtaac gaacagcgta
    11941 ggcaataccg gcaacggcac gcgcgaaatc accatcgacg cgaatctccc cggtctgcgg
    12001 attgacaccg tggcgggcga tgatgtggtc aacattatcg agcacgggca ggcgctggtg
    12061 attaccggca gcagctccga tctggcagcg ggcagcaacg tcacgctgac cattaacggg
    12121 caaacctatg ttgcggcggt actggcggat ggctcatgga gcgtcggcgt tccggcggtg
    12181 gatgtcagcg cctggcctgc ggggacggtc acgattacag ccagcggcaa caccaccgcc
    12241 ggaaacccgg tgagcgttac gcatccggtg actgtcgatc tcactgcggt ggcggtcagc
    12301 atcaacgcca ttactgccga tgacgtgatt aacgctgccg aaaaaggcgc ggcgttaacg
    12361 ctctctggca gcacttctgg tgtcgaagcc gggcaaacgg tcaccgtcac ctttggcggc
    12421 aaaacctaca ctgcaagcgt cgcggcgaat ggttcctgga gtaccacggt tccggcgaca
    12481 gatatggcag ccctgcgtga tggcgatgcc agcgcgcagg ccaacgtcag caatgttaac
    12541 ggcaacagcg ccaccactac ccacgcttac agcgtcgatg ctaccgcgcc aacggtgacc
    12601 attaacacca ttgcgggcga cgacattctt aacgccgccg aagccggagc ggcgctgacc
    12661 atcactggca gcagcacggc ggaagcgggg cagacggtga ccgtcaccct caatggtgca
    12721 aactatactg gcaccgtaca gacggacggc agctggagca tcagcgtacc gccagccgac
    12781 ttaagcgccc tgaccgccag caactacacc gttagcgcgg cggtgagcga caaagtcgga
    12841 aacccggcct cggttaatca caacctgacg gtggatacgt ctgttccggt cgtcaccatc
    12901 aacacggtgg caggcgatga tgtgatcaac gcgacggaac acgcccaggc gcagattatc
    12961 agcggctccg ccacgggtgc ggcaaccggc agcgccgtga cggtgactat cggcacaaac
    13021 acctttacca ccgtactgga tgccagcggt aactggagcg tcggcgttcc ggcaagcgtc
    13081 gtctcggcgc tggcgaacgg cacggtgacc attaacgcca gcgtcaccga tgccgccgga
    13141 aacagcggca gcgctacgca tctggtgacg gtcaatactg gccttccgtc aattaccttt
    13201 aacgccatca gcggcgataa cgtcctgaac gccgatgaaa aaggccagcc gttgaccatt
    13261 agcggtagca gtacggggtt ggcgaccggc gcacaggtta ccgtcacact caacggtcat
    13321 aactacagcg ctaccaccga cgcggcgggc aactggacgt taaccgtgcc ggtgagcgat
    13381 ctggcggctt taggccaggc caactatatc gtcagcgcca gcgccaccag tgcggcaggc
    13441 aacatcgcca gcagccaggc gaatttactg gtcgatagcg gcctgccggg cgtcaccatc
    13501 aacaccgtgg cgggcgatga tattatcaac gccgccgaag ccggggccga tcaaaccatc
    13561 agcggggtgg tcactcgtgc cgccgctggc gatacggtta ccgtcacgct gggcggcaac
    13621 acttacacca ccacggtaca gggcaactta agctggagta tcagcgttcc ggcagccgat
    13681 ctccaggcgt tgggcaatgg cgatttgacc attaacgctt cggtcaccaa cgcgaatggc
    13741 aatacgggca gcggtacgcg ggatatcacc attgacgcta acctgccagg gctgcgcgtg
    13801 gatacggtag cgggcgatga tatcgtcaac agcattgaac acgggcaggc gctggtgatc
    13861 actggcggca gtagcggcct gaatgcaggc gtgccattga cgatcacgat taacggcacg
    13921 gcttattccg ccacggtaca ggcagacggg agctggagcg tcggcattcc ggcgacaaac
    13981 gtcagcgcat ggcctgcggg ggcgttaacc gttgacgtgg caggccagag cagcgccgga
    14041 aatccggtca gcgtcagcca tccgttcacc gtcgatttaa cggcggtggc aatcagcatc
    14101 aacaccgttg ccagcgacga cgtaattaac gccgccgaaa aaggcaccga cctgaccctt
    14161 tctggcagca ccagcggaat tgaaagcggg caaaccgtta ccgtcacttt tggcggtaaa
    14221 acctacactg caagcgtcgc cgccaacggt agctggagtg tgaacgttcc agcggcagat
    14281 ctggcaactc tgccagacgg cgcggcgaac gtgcaggcca gtgtcagcag cgcgagcggt
    14341 aacagtgcct cggcgaccca tgcgtacagc gttgacgcca gcgcaccgac gctcaccatt
    14401 aacaccattg ccagcgacga tattcttaac gccacggagg cgggaagccc gctcaccatc
    14461 agcggcacca gcaccgccga aaccgggcag acggtcaccg taacccttaa cggcgcaacc
    14521 tacagcggta atgtgcaggc agacggtagc tggagcgtca gcgttccccc ttcagcctta
    14581 ggcgcgctga ccgcaagcaa ttacaccgtc agcgccacgg tcaacgacaa agcgggcaac
    14641 cccggcagca ccagccataa tctggcggta gacaccaccg cgccggttct caccattaac
    14701 accgtggcag gcgatgacat catcaacgat gctgaacacg cccaggcgct ggtgatttcc
    14761 ggcaccagca gcggcgggga agcgggcgat gtggtgagcg ttgtgctcaa cggcaaaacc
    14821 tacacctcca ccctggatgc ctccggcaac tggagcgttg gcgttccggc ggcggatgtt
    14881 acggcgctgg gtagcggcgc acagaccatc accgccagcg tcagcgatcg ggcgggtaac
    14941 agcgatgacg ccagccgcac cgtgaccgtc agcctcagcg cgccagtgat tagcatcaac
    15001 accatcgcgg gcgatgatgt gatcaacgcg acggaaaaag gatctgatct gacgctttcc
    15061 ggcaccagcg atcagcctgc gggtacggcg atcaccgtta cgctgaacgg acaaaactac
    15121 agcgccacca ccgacgcctc cggcaactgg agcgttaccg tgcctgcctc tgctgtcagt
    15181 gcgctgggcg aagccaccta cagcgtgacg gcgagcgtca ccaatgctca gggtaacagc
    15241 agcaccgcca gccataacgt gcaggtcaat accgcgctgc cgggcgtcac cattaatccg
    15301 gtggcgacgg acgatattat caacgccgct gaagcaggca gtgcgcaaac catcagcggc
    15361 caggtaaccg gtgcggcggc gggcagcacc gtcaccgttg aactgggcgg caaaacttac
    15421 accgccacgg tccaggctga tttaagttgg agtgtgagcg tacctgccgc cgactggcag
    15481 gcgctgggta acggtgagtt aacggttaat gcctcggtga ccaacgccgt tggcaacacc
    15541 ggtagcggca cgcgcgatat caccatcgat gccagtctgc ctggcctgcg ggtggatacc
    15601 gtcgcgggtg atgatgtggt caatatcatc gaacacagtc aggcgcaggt gatcaccggt
    15661 agcagctccg gctttgcggt gggcacggcg ctgaacgtag tgataaataa tcaaacttac
    15721 gccgccacgg tgctggcgaa cggcagctgg agcgtcggcg ttcctgcgac ggatgtcagt
    15781 aactggcctg cgggaacgct gaatattacc gttagcggcg cgaacagtgc cggaacgcaa
    15841 accagcatta cccatccgct gaccgttgat ctcaccaccg tcgccgtcag cataaacagc
    15901 atcaccagcg atgatgtgat taacgccgcc gaaaaaggcg cggcgctaac gctctccggc
    15961 agcacgtccg gcgtcgaagc ggggcaaacc gttaccatta cttttggcgg taaaacctac
    16021 accaccacgg tggcggcaaa cggtagctgg agcaccaccg ttccggcggc ggatatggcg
    16081 gccctgcgtg acggcgatgc cagcgcccag gtgcgggtga ctaacgtcaa cggcaacagc
    16141 gccacggcaa cgcatgaata cagcgtcgat agcgccgcgc caacggtcac catcaacacc
    16201 attgccagcg ataacatcat caatgccagc gaagcggcgg cgggcgtaac ggtgtccggc
    16261 accagcacgg cgcaaaccgg gcagacgctc accgtcacgc ttaatggcac taactaccag
    16321 accacggtgc aggcagacgg cagctggagc ttaacgctgc ccgccagcga cttaaccgca
    16381 cttgccaata acggctacac cctgaccgcc acggtcagcg atttggcggg taaccctggc
    16441 agcgccagca aaggtgtgac cgtcgatacc accgcgcccg tcatcagctt taacaccgtg
    16501 gcgggcgacg atgtgattaa cagagttgaa cacactcagg cgcagattat cagcggcacc
    16561 gccacgggcg cggtggcggg cgaccgtctg gtggtgacca tcgccgggca gcagtatgtc
    16621 accagcaccg atgccagcgg caactggagc gtcggcgtgc ctgccagcgt gatttccggc
    16681 cttgcagatg gcacagtgac tatcagcgcc actatcaccg atagcgcagg caacagcagc
    16741 acccagacgc acaacgtaca ggtaaacacg gcggcagtgt cgctctcggt cagcaccatc
    16801 agcggcgata atattattaa cgccgccgaa gcgggcagtg cgctgaccct gagcggcacc
    16861 ggaacgaact ttgccgcagg tacagtggtg accgtgttgc ttaacggcaa aggctacagc
    16921 gctaccattc agaacaacgg gagctggagt gtgaacgtgc cggcggcaga cgtcgcggca
    16981 ctggcggatg gcaccagcta cacggttagc gcctccgccc aggatagcgc cggaaacagc
    17041 gccacggcct cgcgcagtgt ggcggtggat ctcaccgcgc cggttatcag cataaacacg
    17101 gtttcaacgg acgaccgact caacgccgcc gaacagcagc agccgttaac ccttaacggc
    17161 tcgaccagcg cggaagtagg gcagacggta accgtcacct ttggcggcaa aacttatacc
    17221 gccacggtgg ccgccaatgg cacctgggcg ctgaacgtgc ctgcggcaga tctggccgcg
    17281 ctcgggcagg gcgcacagac cattaccgcc agcgtgaacg atcgcgccgg taaccccggt
    17341 caaaccacgc acgccctgac cgtcgatacc gttgcgccaa cggtcaccat cgccacggtg
    17401 gcgggtgacg atattatcaa caacgccgaa cagctcgctg ggcaaaccat cagcggcacc
    17461 accaccgctg aagtgggcca gacggtgacc gtcaccttta acggtcagag ctggactgca
    17521 acggtcggca gcggcggaag ctggtcggtg tttattccgg cgcagcagtt tgccggatta
    17581 agcgacggca gctacaccat tagcgcgacg gttagcgatc aggccggaaa ccctggtagc
    17641 gccagccgtg gcgtgacatt gaacggcgac gtcccctccg tcaccatcaa cacctttgcg
    17701 ggcgatgatg tggtgaatgc ggcggagcac ggctcgtcgc tggtgatcag cggcaccacc
    17761 accgccccag tcgggcagac gctgaccctg accttaaacg gcaaaaccta cactaccacg
    17821 gtacagaccg gcggtagctg gagctacacc ctcggcagcg ccgatgtcac cgcgctggcg
    17881 gacggcaatg cctacgtgat taacgcctcg gtgagcaacg ccattggcaa caccggcagc
    17941 agcaatcaca ccattaccgt cgacctcagc gccccggcga tgggcattaa tatcgattcc
    18001 ctgcaagccg acactggcct tagctccagc gactttatca ccagcgtcag cccggtggtg
    18061 gtcaacggct cgctcaccgc cgcgcttgcc agtaacgaga cggcgcaaat cagtatcgac
    18121 ggcggcgtaa cctggaccac gcttaccgtt accggcacga cctggcgcta taacgacagc
    18181 cgcacgctga ccgatggtaa ctatctctat caggtacggg tgattgacgc agcgggcaac
    18241 gtcggtgcca ctgacagcca gaatgtggtg atcgacacca tcgcgccaga tccagcagta
    18301 aaaaccatcg ccatcagcgc gatcaccacc gatactggat tgattactaa cgattttgtc
    18361 accagcgaca cgacgcttgc ggtgagcggc acgctggggg cggcgctttc tgccggtgag
    18421 ttcgcgcaaa tcagcattga tggcggcacc acctggcaga atctttcggt aagcggtttg
    18481 acctggacat atcttgacgg gcgtacgctg actgacggca actacaacta tcaggtacgg
    18541 gtgatcgaca ccgccggaaa tatcggcgcg actgccagcc agatagtcac cgtggatacc
    18601 accgcgccgc tggcgagcaa aaccatcgcc attgcgggca ttagcgatga caccgggtta
    18661 agcagcagcg attttgttac tcgcgacact acgcttactg tgcgcggcac gctcggcgcg
    18721 gcgctggcgg cagacgaacg ggcgcaaatc agcctcgacg gcggtgtcac ctggaccacg
    18781 ttaaccgtta tcggcaccag ctggagctat gccgatggtc gcacactgac cgacggcacc
    18841 tggaactaca cggtgcgggt ggtggatctg gcggggaacg ttgggcagac cgcaacgcaa
    18901 aacgtggtgg tcgacaccac cagcccggaa gcggcgaaaa gtatcaccat taccggtatc
    18961 agcgatgaca ccggagccag cagcagcgat tttattacca gcgacacctc gcttaccgtg
    19021 cgcggcgtat tgggggcggc gctcggcgct aacgaattcg cgcaaatcag taccgacaac
    19081 ggcgcaacct gggtgaacgt gaccgtcgcc gcagacggcc ttaactggag ttacgttgac
    19141 ggacgcacgc tcaccaacgg caccaccacc tggcaggtgc gggtggtgga tctggcgggc
    19201 aacgttggcg caaccggcag ccagtcggcg cagattgaca ccgtgaaccc ggcgcaggta
    19261 ctcaccatcg ccagcatcag caccgacacg gggagttcgg caactgactt tatcaccagc
    19321 gatacctcgc tcacgctgac cggttcgctg ggggcggggc ttgccagcgg cgaagtggcg
    19381 cagattagcc tcgacggcgg cgcgacctgg atcacgctca ccaccaacgg cacgcagtgg
    19441 acctacactg acagccgcac gctgaccgac ggcagctacg tttaccaggt gcgggtgctg
    19501 gatctggcgg ggaatactgg cccggtggtg tcgaaaacgg tggtggtcga tacgattaac
    19561 cccaccgcga cgccgaccat tgtgtcgtac actgatgacg tcgggcagcg gcaggggaca
    19621 ttaagcagtt cgcaggccac cgacgacact acgccgttgc tgaacggcgt gctctccggc
    19681 ccgcttgcca gcggtgaagt ggtttacctc taccgtaacg gcctgctgtt aggcgcggta
    19741 acgatggtag gcgcactgaa ctggacctac agcgacagcg ggctggtgag cggtgcctac
    19801 acctacagcg cgcgagtggt ggatctggcg gggaatatca cctcctccag tgattttgtc
    19861 ctgatggtcg atacctctat tccgaccacg ctggcgcaaa ttaccagcca gaccacgcgc
    19921 gatactacgc cgattatcag cggggtgatc accgccgcgc ttgccagcgg gcagtatgtt
    19981 gaagtggtga tcaacggcaa aacctacacc tctgaaccgg gcggtgcggt ggtggtcgat
    20041 ccggcgcaca acacctggta tgtacagttg ccggataccg acgcgctggc ggcttctgcg
    20101 accgcctata acgtcaccgc gcaggtaaaa agctcggcgg gcaacggcaa taacgccaat
    20161 attagcaacg gcacggtgac ggttaacgcg gcgattgatt acacaccgac ctggaccacc
    20221 gccagcaaaa ccaccgcctg ggggctgacc tacggcctcg acacgcacgg aatgtggacg
    20281 gtgctggcaa accagcaggt aatgcaatcg actgacccac tcacctggtc gaagaccgcg
    20341 ctgacgctgt atcagagcgg caacaactac gccaccagct ccatcgccga ttacgaccgt
    20401 aacggcacgg gcgatctgtt tatcacccgt gatgactacg gtacaggcta tatcaacggc
    20461 tttaccaata acggcgacgg caccttttcc agcgctattc aggtcaccgt cggcaccctg
    20521 acgtggtacg gctcgattgt ggcatttgat aaagagggcg acggctatct cgacttctgg
    20581 attggcgacg cgggcgggcc ggactccaac accttcctgt ggaacaacgc aggtacgctg
    20641 gtaggtaact ccactacgtc gaacagcggc ggtagcgcca cggtgggcgg ggcggtgacg
    20701 gggtatcttt cgctcaacga aggttctggc gtcgatctga acaatgacgg caggatcgac
    20761 ctggttcagc acacctacaa cctgaacaac tattacacgc tatcttcgct catcaaccag
    20821 gggaatggca cgtttgtctg ggggcagaac accaccaata ccttcctgag cggggcgggt
    20881 agtggcgcta tgagcagcag cgtttctatg acctgggccg atttcgatgg cgacggcgat
    20941 atggacctct tcctgcctgc cagccaggga agagctaact acggctcgct gttattcaac
    21001 accaacggcg tgctgggcag tccggtggcg gtgggcgcaa cggcaaccac ctacgccagc
    21061 cagtttagcg tggcggtgga ctggaaccac gacggcctga tggatatcgc ccgtatcgcc
    21121 cagaccggac aatcgtatct ttacaccaac gttggtggtg ccagcaactg gacacaatcg
    21181 gccctcggcg gcagccagag cggtaccacc agcggcgtgg cggcaatgga ctacgactgg
    21241 gacggcgcgg tggatgtact ggtgaccaaa cagtcgggca gcgtgttcct gatccgcaac
    21301 accaacacgg tgagctacgg cacttcgcta cacctgcgca tcaccgatcc caacggcatt
    21361 aacgtctatt acggtaatac cgtaaaactg tataactcgg cgggggtgct ggtcgccacg
    21421 caaatcatta acccgcagtc ggggatgggg gttaacgaca cctcggcgct ggtcaacttc
    21481 tacgggctga atgccggaga aacctacaac gcggtgctga tcaaatccac tggcaccacc
    21541 gccagcaata tcgaccagac ggtcaacacc agctggggcg gcttacaggc caccgatgcc
    21601 actcacgctt acgacctcag cgccgaagca ggcaccgcga gcaacaatgg caagttcgtc
    21661 ggcacgggct ataacgacac cttctttgcc accgcaggta ctgacactta cgacggctcc
    21721 ggcggctggg tgtacagctc cggcaccgga acgtggctgg caaacggcgg catggacgtg
    21781 gttgatttcc gcctctcgac ggtgggcgtg accgcgaact taagcagcac cgccgcacag
    21841 gccaccggct ttaacacctc gacatttacc aatatcgaag gcatttccgg ctcgaatttt
    21901 aacgacactc tgaccggcag cagcggcgat aaccaactgg aagggcgcgg cggcaacgac
    21961 acccttaaca tcggaaacgg cggtcacgat accttgctct ataaattgct caacgccagt
    22021 gacgctaccg gcggcaacgg ctcagacgtg gtgaatggct ttacggtagg gacatgggaa
    22081 ggcaccgccg acaccgatcg cattgatatc cgcgagttgt tacagggcag cggctacacc
    22141 ggcaacggca aagccagcta cgtcaacggc gtggcaacgc tggatgcgca ggctggaaac
    22201 atcggtgatt ttgtcaaagt cacccagagc ggcagcgaca ccatcgtgca gatcgaccgc
    22261 gacggcacgg gcggcacttt tgcggcaact aacgtggtca cgctgacggg cgtgcatacc
    22321 gacctcgcca ctttgctggc gaatcatcag ttgatggtgg tgtagagaat gaaaaaaaat
    22381 gcccagtccg tggaagcctg gctggaggcg atgatcgccg tggcgcgtta ttaccgcctt
    22441 gatttttctc aggagaatgt gcgcgcgacc gtcaactggg agcgcgacag taagcgggag
    22501 gagttactca ccgacatggc gcggcagttg gggatggggc ttcggctggt ggagttctcc
    22561 accgactcgc tcaacccgtg gcggctgccg ttgatcgccg tttttgataa tcaacaaatt
    22621 ggcgtgatca cccgccgtga caatcacgac aatatcagca ttcagtttag cggtgacgaa
    22681 gggctggaaa ctacgcttaa cgtcgcggat gttgaggata aaatcgttga actggcgcta
    22741 ctgcgcccgc tcagcgccat ccctgacgcg cgggtagatg actatattcg cccgtatcag
    22801 gcgaactggt tctggagcct ttcgctaaaa gactggcggc gctacggcga tattatgctg
    22861 gcctcgctgg tggcgaacgt gctggcgctg gcggcgatga ttttctcgat gcaggtttac
    22921 gaccgggtgg ttccggcgca atcgtacccg acgctatggg tgctgttcgc cggggtgatg
    22981 atggcgatcc tgtttgagtt ctgtatgcgc atggtgcgca cacatctttc ggatgtgatc
    23041 ggcaagcggg cggatctgcg catttcggat cgcgtttttg gtcacgcatt acggctgaaa
    23101 aataacgtgc gatcgaaatc caccggatcg tttatctcgc agatccgcga gctggaatcg
    23161 gtgcgggagc tgattacttc caccaccatc ggcgcggtgg cggatctgcc gttcttcctg
    23221 ctgtttgtct ttattttatg gatgatcggc ggctggctgg tgctggtggt tttgctggcg
    23281 ctgccgttat tggtgatccc cggcctgctg gtgcaacgcc cgctggcgcg gctggcgaac
    23341 gaagggatgc gcgagtcggc ggtgcgtaac gctacgctgg tggaagccgt gcagtcgatt
    23401 gaagatatta agctgctgcg cgccgaacag cgttttcaga atcagtggaa ccacaccaac
    23461 gacgtggcct cgtccatcag catgaagcaa cgttttctca ccgggctact gctcacctgg
    23521 acccaggaag tgcagtccat cgtttacgtc gtggtgctgt tggtgggctg ctttatggta
    23581 atgaacggtg acatgaccac cggcgcgctg gtcgggacga cgatcctggc ttcgcgcacc
    23641 atcgcgccgc tgtcgcagat atccggcgtg ctctcgcgct ggcagcaggc gaaagtggcg
    23701 cgcaacggac ttgatgaact gatgaagcgc ccggtggatc agccggaaca cggcaaactg
    23761 gtgcataaag cggtgctaca cggcaattat cagttcagca acgctatctt ttattacgat
    23821 gaagaagaga aaattgccga tgtggcgatt ggcaaactca acattcaggc cggagagaaa
    23881 atcgccattc ttgggcgcaa cggcgcgggt aaatcaacgc tactgcaaat gctcgccgga
    23941 atgcgtatcg cccagcaggg gcaggtgctg ctggataaca tcagcatcgg tcagcttgat
    24001 ccggcggatc tgcgccgcga catggggctg ctgagccaga ccgggagact ctttttcggt
    24061 tcgctgcggg aaaacctgac gatggggatg ccggaagcca gcgatgaaga tatcgaacgc
    24121 gcgttaaccc tcagcggggc gctgccgttt gtgcagaagc agaaaaacgg cctcaactac
    24181 atgattcagg aagggggatt tggcctctct ggtggtcagc ggcaaacgct gctgctggcg
    24241 cggctgctca tctctcaacc caatatcgtg ctgctcgatg aacccagcgc ctcgctggac
    24301 gaaatggcgg aagcgcattt aatcgaacaa ctgaaacagt ggattggcca tcgcacgctg
    24361 attatcgcca ctcaccgcac ggcgatgctg caactggtgg accgcattat cgtgatggat
    24421 caggggcgga ttgtgatgga tggcgcgaaa gaggccattt tgcgtgaaca gagcgaaccc
    24481 acggcgcgac gggtggtgtt gcaggagaag aataaggggt ctgccgcatg aacggacaac
    24541 tgcaatacaa ccgtggcctg actgaacccc gcttgccgcg ttcggcgctg gcggtacgcg
    24601 ttaccgctgt gatgctgctc tgttttctcg gctgggcgtg gtacttccag cttgatgaag
    24661 tcaccaccgg cagcggcacg gtagaaccct ccgggcgcga acaggtggtg cagtcgctgg
    24721 aaggcggaat tctgtatcat ctcgacgtca aggttggcga tattgttgaa cagggccagc
    24781 cactggcgca gcttaaccgc accaaaacgg aatccgacgt ccaggaagca atgtcgcggc
    24841 tgtacgcggc gctggcaacc tcggcgcggc tgcgggcgga agtgagtaat aaaccgctgg
    24901 tcttccctga cgaactgaat aaattccccg aacttatcga gtcggaaacg gcgttgtaca
    24961 acactcgccg cgatgggctg aacaaagcca ccaccgggct gacgcagggg attagcctgg
    25021 tcaaccgcga actggcgatg acccagccgc tggtaaaaca gggggcggcg agcagcgttg
    25081 aagtgctgcg cctgcaacgc caggccaatg aactggaaaa caaactcagc gacgtgcgca
    25141 cgcaatacta cgttcaggcg cgcgaagagt tagcaaaagc caatgcggaa gtggaaaccc
    25201 agcgttcggt gatccgtgga cgggaagact ccctcacccg gctcaacttt accgcgccgg
    25261 tacgtggcat tgttcaggac attgacgtca ccacggttgg cggcgttatt gcccccggtg
    25321 gtaaactgat gaccatcgtg ccgctggatg agcaactgct gatcgaggca aaaatctccc
    25381 cgcgcgatgt ggcgtttatc catccggggc aaaaatcgct ggtgaaaata accgcctacg
    25441 attactccat ctacggcggc ctgccgggag aagtggcggt gatatcacca gacaccgtgc
    25501 aggatgaagt acggcgcgat gtttattact atcgggtgta cattcgcact ttcagcaacc
    25561 atctggaaaa taaaagcaaa cagcagtttc cgatattccc cggtatggtg gcgacggtcg
    25621 atatccgcac cggcaaaaaa agcgtgctgg attatctgtt gaaacccttt aacaaagcac
    25681 aggaggcgtt gcgcgaacga tgaacattag cgatgtagca aaaattaccg gcctgaccag
    25741 caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa
    25801 tggttatcga acctacacgc agcagcatct caacgaactg accttactgc gccaggcgcg
    25861 ccaggtgggc tttaacctgg aagagagcgg cgaactggtg aatctgttta acgatccgca
    25921 gcgtcacagt gccgacgtca aacgtcgcac cctggagaaa gtggcggaga tcgagcgaca
    25981 tatcgaagaa ctgcaatcca tgcgcaacca gctgttggcg ctggcgaatg cctgccctgg
    26041 cgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc
    26101 ggggtgaaaa tcagttaatc cccgtttgca ccgaaagaat atgctgcaaa ctttctacca
    26161 taatgcgccc cgactgaaat aagccttcct ggatttcgtt gttaacattc acagcgtgaa
    26221 ccagggaggc taataacacc acttgcaaga aggcaaaaat aaacgcaccg ataatcataa
    26281 tatgcgtcag ggggattttc ctttgctctt tcattttaaa taacccacaa actgaccttc
    26341 cttcattaag gcgacttttc ccgtgtggtg ataaacaatg acgttataaa gacctgactg
    26401 ataaacagtg gcatctttat tgtcttcatc aattgcacat aatgaatcat tgagataagc
    26461 aaagttctta ctgctcattc taaaggtcat gccaaatcct ttataaatag attttttagc
    26521 ggcctgggca tgttgtgcgg cttctgcatt tttaaatgcc tgcgcttctg atgcacttaa
    26581 aatagccgcc tgacgctgtt gttctgcgag ataacaggtg tcatggctga cgccctgcgc
    26641 ctggcaatct gcgatgcggg aagtgggtga actacagcca gccagtaata taaccgccag
    26701 cagggaaaac gatttaatgg agctattcat cattgttaac cttaaataat gtacggaaaa
    26761 ttgcgaataa gaaattgctg ttaaaattag aacgaatatc ccacgccgac acccgcaccg
    26821 aaatcagatt gtgtatccgc agaaatacct gctttaagta ccgtatgcgt tccggcatta
    26881 aaagatgttc ctacagacac cgcagattca ccgttgtacg ttcccgctcc cgctgacacc
    26941 atcatcaagt caccggtttt cacctgtggt tgtgaggcga tagcgatggc agatgcagtt
    27001 cccgcattgg cttctttacg attgtcctca acttcgtttt tcagagaatc gaaatggtta
    27061 ttgagcgtgg taatctgctg gctattatcc gctactcgtt tttgcgtgtt gcttaactgc
    27121 tgatgcgttt gggttacgtc ttgtgtcagg ctgtcaattt gttcatgcgt ttgtgttgag
    27181 tcttgcgcca gacgctgaat ttgttccccg tactgcacac tatcgatatg ctgttgcgtt
    27241 gcctggttag tcgccagagt atctattttt tgtttttgtt ctgctgcagt acgcgcattc
    27301 gcatcatttt gtgctctcag tccggcgtta accatctctt gataatgtgc gttggtctgg
    27361 gcatcgccaa gtgcggaaac cgttttttgc gtcgcgtcga tttgtttctg ctgatcgtcc
    27421 atttgttgct gctgggtgtc agcttgtgcc tgttgcgcat tggcagcggc tgtctgtctg
    27481 gcgtcaatca tgctctgata tcgggcggca gtctgcacat ccccggtggc ggcaacggtt
    27541 ttccgggttt cgtcgagttg ttgctgctgg tcagcaatat cgctgcggtt ctgttcgata
    27601 ttatttttgt tttctgtcac agcaccggaa agtttgacgc cgttcatcag agcggtgttg
    27661 gcgttagcgt tggcatggtc agccgttgac tgggcatgtg cgacatcgcc ttttatgttg
    27721 gtgacgtctg actgcactgc ggagacatca ttacgggtgt cggcgatggc ggctttattg
    27781 gcatcaatat ccgtctgcgc caccataatg gcgtgctgcg cgtcatcagc tttcattgaa
    27841 gcccagttgg cggcatgttg tgcgtcctga atggcggtat cctgcccgtt gtcgcggacg
    27901 atctgttttt gctcgtttga tgccaacagt ttctgctggt cggcaatatc gctgcggttc
    27961 tgttcgatgt tgtttttgtt ttctgtcaca gcaccggaaa gtttgacgcc gttcatcaga
    28021 gcggtgttgg cgttagcgtt ggcatggtca gccgttgact gggcatgtgc gacatcgcct
    28081 tttatgttgg tgacgtctga ctgcactgcg gagacatcat tacgggtgtc ggtgatggcg
    28141 gctttgttgg catcaatatc cgtctgcgcc accgtgatgg cgtgctgcgc gtcatcagct
    28201 ttcagtgaag cccagttggc ggcatgttgt gcgtcctgaa tggcggtatc ctgcccgttg
    28261 tcgcggtcgg tctgcgcttg attggttatc gggttcagcg tattggcggg ctgtacggga
    28321 gaggcagctg tattttcctg aggtgtcgtt gtccggtaat catgcgcaat cagataattt
    28381 tgtgcggctg cactattccc tgtcctttct aaaaatcgac caattttctg atcaatttcc
    28441 gattgcgaca ttcctggctg gattgccgtt aattctgtcg ttgccgcctc aacagtatca
    28501 cccgcaaacg cagatggact taccgcggta gcaacaacga cagccagtaa agacagcttt
    28561 acagttttca tgcatttttt ctctttcaaa gtaagtcaca tatttgtttc tataagcaac
    28621 gtaaaacgtt gttataagaa ttaatctgcg ttgcaatttg atcgaaatct acgccgccat
    28681 ttgtgtgatg taaagcgcat ttttgtctgg tgtaagcgta aacatcacca ataagaaaca
    28741 aaaaatgttt cttatataaa taaaatcgaa aa
//
LOCUS       DAYPHN010000047        28344 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000047 DAYPHN010000000
VERSION     DAYPHN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.047"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(125..1621)
                     /locus_tag="ACG0BW_001997"
     CDS             complement(125..1621)
                     /locus_tag="ACG0BW_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFK7258963.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLSSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMTPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(1841..3127)
                     /gene="dctA"
                     /locus_tag="ACG0BW_001998"
     CDS             complement(1841..3127)
                     /gene="dctA"
                     /locus_tag="ACG0BW_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFK7258964.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(3310..5298)
                     /gene="hmsP"
                     /locus_tag="ACG0BW_001999"
     CDS             complement(3310..5298)
                     /gene="hmsP"
                     /locus_tag="ACG0BW_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFK7258965.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(5380..8853)
                     /gene="bcsC"
                     /locus_tag="ACG0BW_002000"
     CDS             complement(5380..8853)
                     /gene="bcsC"
                     /locus_tag="ACG0BW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFK7258966.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIGVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWTQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHGQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATNNASLNTQRRVALAQA
                     QLGDTAAAQQTFNQLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKIINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(8835..9941)
                     /gene="bcsZ"
                     /locus_tag="ACG0BW_002001"
     CDS             complement(8835..9941)
                     /gene="bcsZ"
                     /locus_tag="ACG0BW_002001"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFK7258967.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(9948..12287)
                     /gene="bcsB"
                     /locus_tag="ACG0BW_002002"
     CDS             complement(9948..12287)
                     /gene="bcsB"
                     /locus_tag="ACG0BW_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFK7258968.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(12298..14916)
                     /gene="bcsA"
                     /locus_tag="ACG0BW_002003"
     CDS             complement(12298..14916)
                     /gene="bcsA"
                     /locus_tag="ACG0BW_002003"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFK7258969.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRTFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(14913..15665)
                     /gene="bcsQ"
                     /locus_tag="ACG0BW_002004"
     CDS             complement(14913..15665)
                     /gene="bcsQ"
                     /locus_tag="ACG0BW_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFK7258970.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYHWILIDLPRDSSQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            complement(15677..15865)
                     /gene="bcsR"
                     /locus_tag="ACG0BW_002005"
     CDS             complement(15677..15865)
                     /gene="bcsR"
                     /locus_tag="ACG0BW_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFK7258971.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            16137..17708
                     /gene="bcsE"
                     /locus_tag="ACG0BW_002006"
     CDS             16137..17708
                     /gene="bcsE"
                     /locus_tag="ACG0BW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFK7258972.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRLWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQKGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQNSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            17705..17896
                     /gene="bcsF"
                     /locus_tag="ACG0BW_002007"
     CDS             17705..17896
                     /gene="bcsF"
                     /locus_tag="ACG0BW_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFK7258973.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            17893..19572
                     /gene="bcsG"
                     /locus_tag="ACG0BW_002008"
     CDS             17893..19572
                     /gene="bcsG"
                     /locus_tag="ACG0BW_002008"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFK7258974.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     SDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19659..19766)
                     /locus_tag="ACG0BW_002009"
     CDS             complement(19659..19766)
                     /locus_tag="ACG0BW_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFK7258975.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNSLNKRK"
     gene            complement(20142..20249)
                     /locus_tag="ACG0BW_002010"
     CDS             complement(20142..20249)
                     /locus_tag="ACG0BW_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFK7258976.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKQK"
     gene            20725..21996
                     /locus_tag="ACG0BW_002011"
     CDS             20725..21996
                     /locus_tag="ACG0BW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFK7258977.1"
                     /translation="MQHNTLPKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(22026..23030)
                     /gene="dppF"
                     /locus_tag="ACG0BW_002012"
     CDS             complement(22026..23030)
                     /gene="dppF"
                     /locus_tag="ACG0BW_002012"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFK7258978.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(23027..24010)
                     /gene="dppD"
                     /locus_tag="ACG0BW_002013"
     CDS             complement(23027..24010)
                     /gene="dppD"
                     /locus_tag="ACG0BW_002013"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFK7258979.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(24021..24923)
                     /gene="dppC"
                     /locus_tag="ACG0BW_002014"
     CDS             complement(24021..24923)
                     /gene="dppC"
                     /locus_tag="ACG0BW_002014"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFK7258980.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(24933..25952)
                     /gene="dppB"
                     /locus_tag="ACG0BW_002015"
     CDS             complement(24933..25952)
                     /gene="dppB"
                     /locus_tag="ACG0BW_002015"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFK7258981.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLNYIWSVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(26103..27710)
                     /gene="dppA"
                     /locus_tag="ACG0BW_002016"
     CDS             complement(26103..27710)
                     /gene="dppA"
                     /locus_tag="ACG0BW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFK7258982.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(28158..>28344)
                     /locus_tag="ACG0BW_002017"
     CDS             complement(28158..>28344)
                     /locus_tag="ACG0BW_002017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7258983.1"
                     /translation="SVASGNGTALIVGCGVNTLFDLHTTHFPAKIAPHISLSINGFVT
                     ETIVFFYICLNLFLRYL"
BASE COUNT         6584 a         7821 c         7231 g         6708 t
ORIGIN      
        1 ttaataatga atggttatct gcgcgctacc gccgtgcccg gaaaaatatc tgcggttata
       61 tcacggtaac taatttatta ccgttaatcc cggcagcgca gaagaaacgc tgccgggtta
      121 tttattactg tgcgggtgga atatccgtca cttcaggatg aacgtcatcc gtggcgacag
      181 aggtggtcgc agcggcagta gatggggtca tgatttgatc ccaggccgcc tgcaacgctt
      241 tcatgttaaa ttccggctcg cctttcggct gcagcagtat taacgccata tcgttcgaca
      301 actgctgacg cagatcctga tttaacatct ccaccgtcag gctgttaagg aagtcctggc
      361 gcagtttctg atattgctcc ggcgcgatat caacgacctg attttgcaac gaacgcatcc
      421 gctgacccat cagaatatcg gtatcagctc gcgcataggc ggcaaacagt ttctgcagct
      481 ccagtttctt ttgcgccact aacgcattga actcttcttc cggcagacct ttatcgcgaa
      541 ccttcgccag ttcacgcgcc accagattaa ggttgctgct cagcttgtcg tttggtgatt
      601 cgatgttaat ggcacactgc gcacgcagat acagcacacg acagtcaaat ccaagaccga
      661 tatctttgct gttactggcg cttaacgctt gctgaacatg ccagaacagc gcctcacggg
      721 ccaggtccgc acgccagtag cgcagcagtg cggctgattc acgaatcggc tgccacggcg
      781 tatcccacat gatggataac cgatcctgac gcaccgcgtc agtcataata ctcaccgctt
      841 ccgcacgcag cggagaaagc gtcggcaccg gagccggtgt ttcacgtttg cctttcagtt
      901 cgccaaacgt tttattgatt tggtcgacga ccgagcgcgc atccacgttt cccaccacca
      961 gcagcgtcat tgcatccggg gtgtaccatt tctgatagaa atctttaatc ttttccgctt
     1021 caacgggttg tttcagcgga tcggcaggat cgtgacctaa caaggttgat cctttcagac
     1081 gatagcgcca ccagccctct ttagtatcgg caggccaggt tgccaccatg tcctgacttt
     1141 gcagcgcgtg gttgatggtt tctggcgtga tagtcagttt gccagtggca tttgccagat
     1201 aagagagcgc ttcttttagc aagtcgttac ggttattggg caaactcaga ttaaacagcg
     1261 tggtgtcata agagacaatt accggcggca tcgggcgttt agggtcgatc ccctgctgcc
     1321 acaatgaacg cgcctgtgct gcttcaaggc caccgctttg cgttaacgca atacgaggga
     1381 tggcgtggct gtaaccgctc tgttgtgtac tttcggcgag cgaaccggta ttaaccagca
     1441 ggcgaatttc aacacgatcg ctgggacgct ggggcgtggt cagcacttgc cactgtaaac
     1501 cgttggaaag cgtcccctgt tgccatgctg gatcaggctg gagcgcatct gcctgcacat
     1561 agccagcagt ggccatcatc agcaaaccgc ccgctaaaag tcgaattttt gtgccctgca
     1621 tgtgaacccc tgatcaacta tcctggtaat aaaaaactgc ccgttgtcgg tcagtctaaa
     1681 tgacgttaaa aacacttcgt gttagaccgc aagaacatga aaatgtcacg gaagaagtga
     1741 aataaacctg aactcaccca ggtggtaagt tcaggcacag gggtcaatta tgcgcaaaca
     1801 cccgcactcg gggaagggag tgcgggcata agtggtgaga ttaagaggat aattcgtgcg
     1861 ttttgccatc cggcgcacga ttattcagca catcgtccag ttttttgtgg tccagttctt
     1921 tcacccactt agcaacgaca atggtcgcta cgccgttacc gaccaggtta gtcagcgcac
     1981 gagcttctga cataaagcgg tcaataccga ggatcagcgc cagacccgct accggcaaat
     2041 ggcccaccgc agagagcgtt gccgccagca cgataaaacc actacccgtt acccctgccg
     2101 cccctttaga agaaagcagc aacacgatta acagcgtgat ttggtggacg atatccatct
     2161 gactgttagt ggcctgggcg ataaacactg ccgccattgt caggtatatc gatgtgccat
     2221 caaggttaaa cgagtagcct gtcgggatga ccagccccac caccgatttg cggcagccga
     2281 gtttctccat cttgtcgagc atacgcggca gcgccgactc ggaagatgaa gtccccagta
     2341 caatcagcaa ttcttcacgg atgtagcgga taaatttgaa gatactgaaa cccgtcgctt
     2401 tagcgattga acccaatacc agcaccacaa acaggataca ggtaatgtag aaacagataa
     2461 tcagctgccc cagctgcacc agtgtgccga cgccgtattt accgatagta aacgccattg
     2521 ccccgaacgc accaataggt gccagacgca tgatcatatt gatgatgccg aagatgacct
     2581 gcgagaaact ttcgatcacg ttaaaaatca gttggccttt gctgcccaga cggtggagcg
     2641 caaaaccaaa cagtacggca aacagcagca cctgcagaat gttaccgctg gcaaatgcgc
     2701 caatgacgct cgccgggatg acatccataa tgaaggcgac gatgccctgg tctttcgcct
     2761 gatcggcgta aaccgctacc gctttcgcat caagcgttgc cggatcgacg ttcattccgg
     2821 caccaggctg cacgacgtta acgatgataa gaccaataat cagcgcgatg gtactgacaa
     2881 tttcaaagta aagcagtgcg actgcgccgg tacgaccgac cgccttcatg ctttccatgc
     2941 ccgcaatgcc cgttacgacg gtacaaaaga tgacaggagc gatgatcatc ttaatgagct
     3001 taacgaaccc gtcgccaagc ggtttcattt gctcgcctat ttcagggtag aaatggccaa
     3061 ggagaatacc aatggctatc gctgtcagga cctgaaagta aaggctttta aacagagagg
     3121 ttttcatagg gtgtccttta gtaaaaccac aggtcttgta aggttatggg gtacctgcgg
     3181 ccttaaaata acacccagac aacatcacag aaatgtacct ggatcataat tgaaacaaaa
     3241 aggttaaaaa gtttgagctg gctcgcacaa accagcactt tttaaagttt tgtaatcagt
     3301 ttggggtagc tacttttctt ccaggtaact ctcttcgaag atttcaatag ggagtgggcg
     3361 agcaaaaagg aagccctggg caataccaac gcccgctttt gccagccagt cgcgttgtgc
     3421 ttcagtctcc acgccttcgg caatcatttg taagttcagg ctttgcgcca acatgataat
     3481 tgcagcaatc atgctgctat cttccggcaa gccttcaaca aacattttgt cgattttcag
     3541 tacgtcgatt ggcaacgatt tcatatgctg cagctgacgc agccccgcat agcccatgcc
     3601 gaaatcatcc agcgccaccc gaactccggc attgcgcagc ggacggagga ttgccaccgc
     3661 agcatgaggg tcgtcaatac gtcggctttc tgtcacttcc agaatcagtg ttcccggctg
     3721 aatgcgatag cgggttaaca gttccagcat atccgccacc atattcgggt gcatcagttg
     3781 cagcgctgag aggtttaccg acaagggcag cataatgccg cgctcttgcc aggcagcaag
     3841 cagtcgacag gactcttcca gcacccagtg accaacggta accatcagcc cacagcattc
     3901 aatgcgatcg attaagccat ccggcagatc ccaactgcca tccggttgct ggatacgcag
     3961 taacacttcc gcactgacca gtttaccgct ggtcatctcg acctgtggct gtaaccaaat
     4021 cgcaaactga tgattttcca gtgcattaag gatatcgctc tcttccgtca accgctgctg
     4081 ggcggcttcc atctgctgcg gatcaaagaa ctgaatctga ttcttgcctt tatggcgagc
     4141 ggtaaatgcc gcagaaatag cacgactgta aagctgttcg gcggtgagat cgccgtagaa
     4201 catcgccacg ccaatgctac agtgcggacg gagttgaata cgttcaatcg gcaggcgctc
     4261 gctcatgata gtgagcactt gctgacctaa agtgattgcg tgccacggtt cctgtacacc
     4321 attggcaatg acagcaaagt cataaccact aatctgcgcg aggatcatac gtggcgacag
     4381 taccgatttg agtttttcca ccagcgtcag cagcagaatt tctcgttgcg cctctttcag
     4441 cacgcccgca gtatcacgca gggtttcaca ggtgatgatc atcagcgcgg tggtttgttt
     4501 acgcgcgaca acctgctcca gcatctccat cagcaaggct ttgttcggca aatccgacac
     4561 cgggaagcgc atcgcgtttt cgttctgttc ttcataatgg cgctgcagca attgctggtt
     4621 gaggttgtaa ctgcgcacca gcataccgat ttcatcgtcc tgatgcagac gtggtaatgc
     4681 cagttggtga ccaacaagct cctgggcagg gatggcgtta agttcgcgag caatattgcg
     4741 taacggatgc aaaatcaggc ggttaatgca ccagctaatc gccaccgtca atattaacga
     4801 caaaagtaag taaatggtca ctaacgttga gagggtgctc atcacgaact tatacatgcg
     4861 gaaggaatcc gcctgtagca ccagataggc aattggctgc ggatttgccg gacgttcgag
     4921 cgagtagaca cccagcgaga tttgaaccgg tagctcaaac aggcgagtaa ccattaccgg
     4981 caccggacgc tctggaataa aacttttacg cagcgcctgg aactggttag gcagcactac
     5041 atcggcacgg ctgaccacgc ctgccggttt aatgctggca agaatagctt ccgcttcggg
     5101 aatatcgcct ttcaaaatag ctgaagataa gggttcgcgg acagagcgag caatgctttc
     5161 cagttgcgta gccgtgttat agcgattctg ctggaccaga tggaacagca aaacggtgca
     5221 aaaaataaaa acgaacacca ggacaacggc tgccaccatt gccatctgct tgattgttaa
     5281 cgagcgactt acgcgcaaat tgtctccccc cggaaatccc agattgccgc ccgagtatac
     5341 ccgatcgcag cggcattaag agaggcgcta tctgaaaact taccagtcgg cgtaaggtat
     5401 cagcggctgc ggcggtaaat ccatgtcacc ctgccatccg gcggcggaat aacgtacgta
     5461 gagcaggaaa tggctgggtg cgtaatcttt cgcctgctgg atatcaattg ccgtgccgac
     5521 aaaccagttg gaagtaacac gtcgttcaag taatgcccgc gccgtatagc cgaagccctg
     5581 actgctgccg ccatcgttgg attgccgcgc agcttcttcc tgccagtcgg tcgggatcag
     5641 attcatcagc ggataacgcg gcatggtttt ggtgcgtgaa tgcgaccacg agccagacgc
     5701 ccccagctcc cacgaccagt tttccgtgcg ctcccgccac atcaccggta tggcaaacga
     5761 caggtattcc tgcgggctgt agtaaccgcc ctgaccgagt gagtagccac tcaggtcttt
     5821 gtcgtaatgc cagatcatat tgttaagacc aatggtcaca cggcgattat tctggttgat
     5881 gatcttatag taatagcccg tcatccagcg cacgcgccag ttatcttcga catttttacc
     5941 ggttaactgg tcaccgctaa gcgatgccca gacgccgttt gcttcacctt tatcgtagct
     6001 caggcttagc cccacgccgt cggcacgcac gccgccccat tttttcccgg tattgctcgg
     6061 agagtctttt tgcccaccaa aggccagcaa agaactggag atgggccgac gatgggcgtt
     6121 aacggtgtaa cccagcggcc cgatatcatc gctgtaactg atgccgccga ccacatccac
     6181 cacgttgaag cccatcggcg tggtgccgat atcccagctc cagacgtcat ttcgccaacc
     6241 gaccgccacg ctggcaccgg aatccgactg gctgcggttg ccgctacagt cctgtaatgt
     6301 acaggtgccc cagttgtcat cccatttgcc atcggcatta gtggagaaac tgccgacgtt
     6361 catattgacg aaatcactac gaaagaacat ccgcccgtca gaatacggcg catccacctg
     6421 caacatggta gtgtgcgctt tcagatcgga gtaaccaccg gtgccgctcg acccccagta
     6481 atcgtgctcg agggtgacgt taagatcctg ctggcgatag aggtccgctg catcgctgcg
     6541 cacgccgcgt ttcagccagt catctttctc gtcgttacgg gtcagccggg taaaggtgtc
     6601 gttatcctgc ggacgcgtcg tggtcacacc ggatgcgacc atcgcgtctt tgtaggtttc
     6661 cagcgcctgc tttggatcgc ccgcctgcgc ttcaaacttc gcaccgtcgc gcagcaccat
     6721 cgcactttcc atcgacggcg gctgagattt tgccagcggg atcaactgat taaatgtctg
     6781 ctgcgccgct gcggtatcgc caagctgcgc ctgtgccagc gccacgcgcc gctgagtgtt
     6841 cagcgaggcg ttattggtgg caggcagctt cgccagctgg ctacgtgccg ccgctttgtc
     6901 accggcagca atatccactt ccgtcagacc aagaatggca tcggcgttag ttggctcccg
     6961 cgtcaggaca ttctgatatg cagcgcgagc ggcggtgtaa tcacgtcgtt gttgcgccca
     7021 gtcagccagc gtgaggtcta tgcgcgtgga aggtggttgc tggcgcagca tcgcttccgc
     7081 ttcagcttct ttgccgcttt ctcgcaggcg attagcggtt tccagtacct gatcgctttg
     7141 cagtcgatta accagctcct gaatattgct gctccactgc gcgcgcggca ggctgttgat
     7201 atgcgccagc gccgctctgt cctggccatg accagagaga tacagcccgt aagcgtaaac
     7261 ctgctccggg tcgttcggct tctgctgcgc cagattacgc attaacgtat cagcctggct
     7321 gcgttgtccg gcctgccaga gatcctgcga aagtcggtaa gtaatccata cgctgccggg
     7381 gtccagcgcc agtcgttgcc gctgaagtgc tgccgcctgc gtccatttgc cctggttttc
     7441 cagtgcctct gcctgctgtg ccagacggtc gttttgcagg ctgcgttcga tatcatcaat
     7501 gcttcgccgc tgactggcag agagcgaggc gataaacgct tcagcttttt ctggcgattg
     7561 ctggcggtat atattcgcca gcccgcgcac ggcgttagtg ttgccgctgt ccatacgcag
     7621 ggtctgctga taatagcgct cagcggcggg gtaatctttt cgcgccatcg ccacatcgcc
     7681 cagccccagc actgcatagc tgtcggtgtt atcgacatta cgcgcctgct ggaacaggcg
     7741 ttctgcccgg tcaggattat tggctttcag cgcagcatcg ccctgctgga tcgccagcca
     7801 gtagcggttt actttcagga gactgttcca tttgtcgttg ttgctgctgt gcggatccag
     7861 ggcgagggct ttttccagat tcgccaccgc attggcgcga tcgcctttct gagaatacgc
     7921 ctggcccagc gcccccagag cctcactgtc tttcgggttc gcccgcactg cctgttgtag
     7981 ttcgggaatg gctttacccg ccataccaga gtccaccgcc gctaaacctt gcgcacgagc
     8041 gcggaaagca gggtcggcca gctgtttttg ctgttctgcc agttgcgatt gtgcagccgc
     8101 cacgctatcg ccatcactaa agatcgagag atattttttc agcgccgaca cactggcatc
     8161 gctgacgggc atgtctttaa tctgcccgta ccagatttta gaggcccctt cgcgcccggc
     8221 gtttgatttc gccatctgtt ccaggacggc aaaaccttcg tcacggcgat cgctactaaa
     8281 cagcaatagt gccagattgt tttgcaggcc cgtattgccc ggcgtatccg cattaatgcg
     8341 ttttagctga ttaatcgctt cgccacggcg agccggaatt ttcgccaccg tactccagta
     8401 ctcgacgcca atgtcacctt ccggcggcgc accgttgaac agtttgttgt aactcgccac
     8461 agcttcttct gcatgaccag tagtcgcctg caatcttgcc tgttgcagtg cctgacgacc
     8521 atccggcgtg gaaagcaaca ttgtggtccg cgacgattta tacgcatttg aactcggcgc
     8581 taactgcgac agccgatcga gctgtttttg cgcgccatca atatcgccct gacgtaacag
     8641 agaacggaaa cgggcggcaa cgacgtccgg gttattcgga tcaataagtt ccagacgata
     8701 caacgactgt tgcaccaggt cttcacgatg ggtcgcttcg cctaaccgaa cttgctccag
     8761 caactgttgc tgagcggttg gtgctgcctc gaccatcggc atgacggcca gaccgaggga
     8821 aagcgtgaat atgtttagtg tgaatttgcg cattcctggc cccagtcagg taataactca
     8881 ccttttgtcg agaagcggaa acggtgttga tcccagcctt gtccaaacag ggtcagcaca
     8941 tagttgtaat aggcatcgct gccgggaaag ttatcggcca cgcgctggcg ctgaacggcc
     9001 tgcgcatcgc ggttttgtaa aaagggcagc atggcggcag aaaaaccgac cggtccttta
     9061 ccctgcgctt tccccgtagc cacatccact ttttctggcg gatagccgtt tttctcggtg
     9121 aatgtcgcca tcggtttaaa ccggttgagc atccgcgctt tttgcggatc gctgtcaggc
     9181 atcatgccta cccacatgta aacgcggatc gcgtcgtagc tgctgatcaa tgttttttcg
     9241 gcttttagct gccagccttt gtctttctca tagcgcaccc agtctggcga aaagcctttc
     9301 ggggcggttt ccagcaataa acgttgattg gtttcgcgca gcgtggtcca cggtgcgcca
     9361 aagcgggtga aatactgcgc cagcgtcggc ggcaggtagc tggggttaaa acgccagctg
     9421 ttatcctctg caaaacccac tttgcccggc aacaacatgg aacccagccc aggcaccgtc
     9481 accacttcct cccgcgcaat acgttttaac aacgcgctgc cgatgtcggt ataacgctgc
     9541 tctttccaca aacgccccgc ctccagcaac gaccaggcca tccagacatc accatcggaa
     9601 gccgaattgc tgtccagcac ttcccactta ctgttctctt tcttgcccca cagccatgcg
     9661 ggcaaacgtt cttttaaaga accctgagcg agattgttct gcgtccagtc gagaatatta
     9721 tcgaaagctg cacggtcgtt agccgccagg gcaaagaaca tgccgtaact ttgtccttcg
     9781 gaggtggtga ttttgcgcgc gtcgctgggg tcgatgacgc gcccttcctg actgatgtaa
     9841 tcctttttaa actgctccca ggcaggccag gtacaggctg cctgaacact aaaggcagcc
     9901 agcagcagca tcgtcacgag tccactacgc aacacattca tcttcaatta ctcgttatcc
     9961 gggttaagac gacgacggct gataatacgc agcagacgcc acagtaccca tgccagcagc
    10021 acgacgctga ttgccgccag caccgccagc agaatcggat ggtttgccag cgcataccat
    10081 aagcgttcga accacggcag atggccaacg taatagacgt cgccaacgcg taggctattg
    10141 ataccggact cgcggatcac cgcgaccgaa ccgaacatgg tggcgcgttt gccgctatcg
    10201 ttcaccgcat cgttaagcat ttcataaccg cgtgggctat ctgccagcag cgcaatcacg
    10261 ctgcgctggt cgttatacgg cgactggaag ccaatcaccg ccgccatcgc accggaagag
    10321 gtcagcgttg actgggtttc tgccgcgcga tcgctctcgt ccggaacaat gccggggaac
    10381 ggggtctggc gcatcggcgt tttcacccag ctttcggtcg cctgcaccaa caggtcgatc
    10441 tgcttgtcgt cttttaattt gtccgggata ccaccgatga tcatgatgtc ggcatctttg
    10501 ccctgaatgg tgctgccatc atcggtcacc gtcagattaa tcgccgggaa gcccgtctgt
    10561 gcaccgataa aaccaacagt attcagcaag gtttccatct gtgcttcgtt aggcgttttc
    10621 ggcatcacgg tgatggtttg tgacagatcc gccatccggc tgaacgggaa gcccgcgtta
    10681 gcaaaggcgc gtagatccgg catcgggatg aagtggtaat acttcgagaa gtcgatggtg
    10741 gaatcatcac caatcaccac atgattctgc accggctgga aggtaatgca gttatccacc
    10801 gaaccgcccg gcatcgggtt catatactca aagtcaaagc gcagctggtt ggtcgcgccc
    10861 agtttcagcg ccggaataga gacatccgtt ttgccatcca gcaaaccttg taataccgga
    10921 atccgcagca gcaggcgatt cgcctcctgt ttgctgctca ggttgaagga ttgcaggaac
    10981 tggttattca ggctgatatc catccgcgaa ctgtctttca ccggcggcat ggtgtagcgg
    11041 taattaatat ccatatcaat gccggtactg cgcatcaggt agagatctgg cggcaggttt
    11101 agcgaaacat taatcgctgc tggctcaaga ccgctggatt gcaactgttc ttcataggtt
    11161 ttcagttcac caaaggtgac cggacgatcg gtacgcaccc agttcggcgc atcgtacggc
    11221 ttacgcggta gcagcggttt cacttcattc actaccacgc tttcaccacg gaacaggatg
    11281 ttaccctgag cgataccttt cgctgcctgc aacaggtctt tgtcgtcacg accaaacacc
    11341 accagcagtt tgacgtaagg attctgcgga tggttgatca tctcaatcac cggggctttt
    11401 accgccggat gatcgcgcag gaagtccggc cgtttgtcgt tggttgcaaa gacaatggca
    11461 ttccgatccg gcagttggtt atagagcacc gggaagttct gcccacgcca gccagaacgc
    11521 gaaccaaacc acgaggcaac aatggcagat gcttgttgca gcccgacatc cggcgcaccc
    11581 gcaaagacca tcggcaaggt gttggtgcgg ttatcacgcg ggtcaaagaa tggtaccggg
    11641 aagtgtgaca gatcattctt cacattcagg gtctgatagg tcagatccag tccactgctc
    11701 cgcccaacat ccagccaaag cgtggtgctg gccgggtttt cgcacacgtc ctgataatga
    11761 ccgacaaact ccagccgcac acggttgaag tcggtaataa acagtgggtt aattggcatt
    11821 tgtgccagcg tttttttacc caactgttct ttggtcactg gcagcacgcc catcagttca
    11881 tcattgagat aaacctttaa ctgcgactgg acaggcagta acgatggcga tggcgtgtat
    11941 tcgagattga gcatcgcctt cgttaccact tcatcgctgc gcataccaaa ctcaatgctg
    12001 ccgttcgggt taatgccacg tagcaccatg ctgcccggcg gcggcgcaat ttgcgcaaag
    12061 gtcagcttca catcacgcga aggaccgttc tgcgccacga ctggcgcatc agcgccctgc
    12121 acgcccggca tcacttgccc aacctgcgga ttttgttccg tctgggcggc tacagctggc
    12181 tcagcattga tcagtggttg cgttgctggc gtcgcctgcg tcatgaaaga ggggaacgca
    12241 ctcatcccca tagccactgc acaaatccag aatagttttc ttttcatcgc gttatcatca
    12301 ttgttgagcc aaagcctgat ccgatggttg tgccgtttcg ctccgctccg ggcggcgcgg
    12361 aataaacgat acaacccagg aaaccagaga agtcagcaca cggaatatgc ccttcaccga
    12421 agaaggcgca aactccgcca gatggcggta gccacggaag ccgagcttca gaatatccag
    12481 cagactttcc agcggcttat cttccggata gctgtcctgc cagagcgccc atgtatccgc
    12541 acgggcaaac gtacactgca caaaatcgat atgttgctgg gtggtgagcg gcattaattt
    12601 cagcccaact tcattaccca tcacgcgcgc cacctgggtg gggaagacgt attcctgctg
    12661 accgcgttta agcaacagat tcactttctg cccttccaga atctgcgcct gaccgttgat
    12721 cttgatcccc aaaccaccgt cggagaaatc ctgaacggta catgagaaga ggtgaccatc
    12781 ttcgcgggca attgccgcgg gcatcgtcat ctccacgcgg tgcgatcggc gtacctgttt
    12841 gctttctacc gataccgcaa ctgcgccgcc aagaacaatc aggttgtaga atacccacac
    12901 catactgacg accacggtga gcatctcggt tggcgggcca tagaagtagc gccagatgcc
    12961 taccgcaacg cccaccaggt tgagcagaac aaggaagatg tagggccgcg agatcaccca
    13021 gtcgacgtac tcttcttcca ccagtccacc tttggcggtg acgttaaatt tgcctttgtg
    13081 cgggttaatc agcgccacca gcgtcggtgg tgcgatatac cacgccagca ccgtttcgta
    13141 gatttcactc cagaaagagt ggcgatattt gccctggatc ttggagttgg tcaggctggc
    13201 atggatcata tgcggcagca cgaacagggc aatcatcaac gctggcgcat agatgatgta
    13261 ggcatgaagc agcaggaacg ccagcggcgc agtcaggaag atcagccgtg gaatgcccga
    13321 caagaagtgg aacatggcgt tgacgtaaca tagccgctga gcaaacttca gccctttacc
    13381 ggtgagcggg ttatcgagac gaaagatttg caccatcccg cgcgcccagc gaatacgctg
    13441 accgatatgc gccgacagac tttcggtcgc cagccccgcc gcctgcggaa tacgcatata
    13501 tgcagaggtg tagccacgac ggtgcaaacg cagagaagta tgcgcatctt cggttaccgt
    13561 ttcgacagca atgccgccaa tctcatccag cggcttacgg cgaatcaccg cacatgaacc
    13621 gcagaagaag gtggcgtccc acatatcgtt gccatcctgc accagaccat agaacagcgt
    13681 gccttcgttc ggtgttttac gaaaacgccc caggttgcgt tcaaacgggt ccggtgagaa
    13741 gaagtggtgc ggcgtctgca tcatcgccag ctgtttttct ttcaggaacc agcccatggt
    13801 catttgcagg aacgatcgcg ttggtacgtg gtcgcagtcg aaaatcgaca cgaactcgcc
    13861 tttggcatat ttcagcgcat tgttgatgtt gcccgctttc gcatgttcat gagtggtacg
    13921 ggcgatatat ttcaccccca cgttttgcgc aaactggcga aactcttccc tgccgccgtc
    13981 atcaaggatc cagatgttca gcttgtcttt cggccagtcg atacccagcg aggcgtaaat
    14041 cgtatttttc accacgttga gatcttcgtt gtaagtcggg acaaagatat ccaccgacgg
    14101 ccacagcgac atatctttcg gcaagggcac cggctgacga ttcagcggcc atactacctg
    14161 gaagtagccg agcaccagca caatccacgc ataagtttcg gcaaacaaca ggattaagcc
    14221 gcacaccagg ctgaccggat cgtcccagtt cagcgtagag gtgtagcgcc accagatata
    14281 acggcaagaa acggtcagcg acagcacaat caacattagc gccgagaagc gccccggcat
    14341 ccgccgtacg atcagcgcta caccccacag cagcatcagg aagataaact gcgccagcgg
    14401 gttaaacggc tgggtaacgc agattaacgc cagaatcagc gagaaggtga cgatgatacc
    14461 gaggatcaac cgccgcgccc ctgcgctcaa atggccgagt tcttttttct catcgagatg
    14521 ctgtgtttta tggctaacgc gctcaggcag ctcgttcatc cattgatggt aacgtccacg
    14581 aatattttgt agacctgaaa atgtccgcct gcgcggtttc ggcgtttctt tgcgcgatgt
    14641 accgatcagt aaccagcatg tttgaatgag ataacggacc gggtccagcg gacgcggacg
    14701 cgaggcgttg atatgcggat acaggttttt atgttctgcg cgaatacgct gccagcgcgg
    14761 gtgctccagc ggaataaaaa tccaggccag gatcaaccaa aaacagccga gcgtcgcgct
    14821 gaaagccgac gcgccgtgac gacgataatc gcgataacgc ccgataagcc gcgcgttgac
    14881 cggcgggata agcaaccacc gggtcaggat actcatgatt tactcccgac tggcgttttc
    14941 agcccggaat agttcaacag gcaccagtta gccagcgtca gtatctcttc agccgccagc
    15001 gcatcactgc gatattcacc gactggttgc ttagccgcca ggcattcagc catcgcttca
    15061 tcacgatgaa tgagcatcgg cagtaatcgg cgctggcttt gcaaccaaag ctggtaaata
    15121 tcgtcctgaa cttgactgcc gatacggaag tcattaatca aaatatgcgc gccatccggc
    15181 agagcctgct gatgcagccg gatatggcag ttggcatcca cattgacgat tgccagcgag
    15241 tgatcgcaca aactcagcag ctggtgggtt atctgcgagg aatcacgcgg taagtcgatt
    15301 aaaatccaat ggtaacgccc gctggctttt agttgctgta agccggagca aatatcgctc
    15361 agccgggttt gccagtgctg tggattttct tgttcttcaa tggataactg accaaaaggc
    15421 agcaaatcga gttgcgaggt atagcgcaac ccagcgtcac gccagtcctg gccatccagc
    15481 atagctctgg cccagccctg acggtgggta aaatcaacgt taaatgacag gcgcaacaag
    15541 ttgtccgggc aggcatcgac caccaggaca ttttctccca acatttgtaa tgaccaggct
    15601 aatgcggcgg tgatggttgt tgtccccacg cctccccgca ccccctgcaa tcccagtacg
    15661 gccattcacc aatcccctac ttttgttgcg caaactctgc cagcaacggc cagcgtttta
    15721 atgccgcggc caactgttcg cgttgggaaa tatcggcata atcaatatca ggcagtgaaa
    15781 atgcctgctt taacgccaaa atatcattct ggaagatata gcctatcgcg ggatcaggca
    15841 gagtatctgg ttcattgtta ttcatttgct tgatccctat gaaataaccg taccgttcat
    15901 gagacaattc taaccgtgag catttcgccg gtttattgtt tacggcgaga cgggaatttg
    15961 ctcacttttt gactttccgt tacgcaacaa aaaaagttaa aaacgaaaag actaaaatca
    16021 cacgtcttgt gacctgctaa attgattcac atgctaaatc tgataagttt taatttcaat
    16081 ggtaggttta tttcttagct ttcgctagta aactgataat cagacaaaat gatgacatga
    16141 gggacattgt ggaccctgta ttctctatcg gtatctcatc attatgggat gagctgcgac
    16201 atatgccagc aggcggcgtc tggtggttta acgtcgatcg ccatgaagat gctatcagtc
    16261 tggcgaatca aacaattgca tcccaggctg aaaccgcaca cgtcgcggtc attagcatgg
    16321 acagcgatcc ggcgaaaatc tttcaattag atgattctca aggaccggaa aaaataaaat
    16381 tattttcaat gctaaatcat gaaaaaggtc tatactattt ggcccgtgat ttgcagtgtt
    16441 ctattgatcc ccataattac ctttttattc ttgtttgcgc aaataacgca tggcaaaaca
    16501 ttcctgccga gcggcttcgc ttatggttgg ataaaatgaa taaatggagc aggttaaacc
    16561 attgttcgct tttggtaatt aatcccggaa ataataacga taaacaattt tcattgttgc
    16621 ttgaggaata ccgttcactt tttggtcttg ccagtttgcg ttttcagggc gaccaacatt
    16681 tgctggatat tgccttctgg tgcaacgaaa aaggggtcag cgcccgtcag cagcttagcg
    16741 ttcagcaaca aaaggggatc tggacattag ttcaaagcga agaggcggag atccaaccac
    16801 gcagcgacga aaaacgcatt ctgagtaatg ttgctgtact ggaaggtgcg ccaccgctat
    16861 cggaacactg gcaactgttc aacaataacg aagtcctgtt caatgaagcc cgtaccgctc
    16921 aggcggcgac ggtggtcttt tctttacagc aaaatgcgca aatcgagcca ctggcccgca
    16981 gcattcatac cctgcgtcgc cagcgcggta gtgcgatgaa aatcctcgta cgggaaaata
    17041 ccgcaagcct gcgcgccacc gatgaacgtt tgttattggc ctgcggtgca aatatggtta
    17101 tcccatggaa tgccccactc tcccgctgtc tgacgatgat cgaaagcgtg caagggcaga
    17161 agtttagtcg ctatgtgccg gaagatatca ctaccttgct gtcaatgacc cagccgctca
    17221 aactgcgtgg tttccagaag tgggatgtgt tctgtaatgc cgtcaacaac atgatgaata
    17281 accctctatt acctgcccac ggtaaaggcg ttctggttgc cctacgtccg gtaccgggta
    17341 tccgcgttga acaagccctg acgctgtgtc gccctaaccg caccggcgat atcatgacta
    17401 ttggcggtaa tcggctggtg ctgtttctct cattctgtcg gattaacgat ctggataccg
    17461 cgttgaatca tattttccca ttgccgactg gcgacatttt ctcaaaccgt atggtctggt
    17521 ttgaagatga tcaaatcagt gccgagctgg tgcagatgcg cctgcttgcc ccagaacaat
    17581 ggggcatgcc gctgccttta acgcaaaatt ctaaaccggt cattaatgcc gagcacgatg
    17641 gtcgccactg gcgacgaata ccagaaccaa tgcgactgtt agatgatgct gtggagcgct
    17701 catcatgatg accatcagcg atatcattga aattattgtc gtttgcgcac tgatattttt
    17761 cccgctgggc tatctggcgc ggcactcttt gcgacgcatt cgcgacacct tacgtttgtt
    17821 ctttgctaaa cctcgttatg ttaaaccggc cgggacgtta cgccgcacgg aaaaagccag
    17881 ggcaaccaaa aaatgactca atttacgcaa aataccgcca tgccttcttc cctctggcaa
    17941 tactggcgcg gcctttccgg ctggaacttc tattttctgg ttaagttcgg cctgttgtgg
    18001 gcgggatatc ttaacttcca tccgctcctc aatctggtgt ttgccgcgtt tctgctgatg
    18061 cccattccgc gctacagcct gcatcgctta cgccactgga ttgccctgcc gatcggcttt
    18121 gctttgttct ggcatgacac ctggttgcct ggcccggaaa gcataatgag ccagggttcg
    18181 caggtggcgg ggttcagttc cgattattta atcgaccttg tcacacgctt tattaactgg
    18241 cagatgattg gggccatttt tgttttatta gtggcctggt tattcctgtc acaatggatt
    18301 cgcattaccg tttttgtggt tgccattctg ctatggctga acgtacttac cctggcggga
    18361 ccaagtttct ccttgtggcc tgccggtcaa ccgacgacca ctgtaacaac gacgggtggt
    18421 aacgcagcgg caaccgttgc cgcgacgggt ggcgcaccgg tagtgggtga tatgcccgca
    18481 caaacagcac cgccaacaac ggcgaacctt aacgcctggc tgaataattt ctataacgca
    18541 gaggcgaaac gtaaatcgat cttcccatct tcgctgcctg ctgatgctca gccatttgaa
    18601 ctactggtga ttaatatctg ttcgctttcc tggtcggata tagaagccgc cgggttgatg
    18661 tcgcatccgc tgtggtcgca tttcgatatt gagttcaaga actttaactc cgccacctcc
    18721 tacagtggcc cggcggcgat ccgtttactg cgcgccagct gcgggcagac ttcgcacact
    18781 aatctgtatc aaccggcaaa taacgactgc tatctgtttg ataacctgtc gaaactgggc
    18841 tttacccagc acctgatgat gggacataac ggccagttcg gcggtttttt gaaagaagtt
    18901 cgcgaaaatg gcggcatgca gaccgaattg atggatcaaa caaatctgcc ggttattttg
    18961 ctgggctttg atggttcgcc ggtttatgac gataccgccg tgcttaaccg ctggctggac
    19021 gttaccgaaa aagataaaaa cagccgtagt gccacgttct acaacacgct tccactgcat
    19081 gacggcaacc attatccggg cgtcagcaaa acagcggatt acaaagcgcg ggcgcagaaa
    19141 ttctttgatg aactggacgc cttctttact gaactggaga aatcgggtcg taaagtgatg
    19201 gtggtcgtgg tgccggaaca cggcggcgcg ctgaagggcg acagaatgca ggtatctggc
    19261 ctacgtgata tccctagccc gtctatcaca gacgtccccg ttggggtgaa attcttcggc
    19321 atgaaggcac cacatcaggg ggcaccgatt gtcatcgacc aaccgagcag cttcctggct
    19381 atctccgatc tggtggttcg cgttcttgat ggcaagattt tctccgaaga caatgttgac
    19441 tggaaaaaac tcaccagtgg gttgccacaa acagcaccgg tctccgagaa ctcaaatgca
    19501 gtagttattc aataccagga taaaccgtac gttcgcctga acggcggcga ctgggtgcct
    19561 tacccgcaat aagaacgaaa aaggccgcag agtttcaccc ctgcggcctg atccgggcgc
    19621 aaattgccat tacggcagcc tgacgcccgc atgacacgtt acttccgctt attcagcgag
    19681 ttcacgatca gactggcaag aatgccagca atgaccggag ccgctaaatc atgccagaag
    19741 gccatgccca gttctgcgag cgtcatatag ccgcctctgt tgtaatgaca acgtttcgcg
    19801 gctattcttg agttgtttgg gttcaagatt agcccccgtg atgttgtcag gtgcatacct
    19861 gcaacgtgcg ggggttttct ctctccagca accaatgcca ccagggatta agcccccgca
    19921 acattgcgcc tcaccggaac ccttccggct tgccgctgat tctacggcca tttttgttcg
    19981 tcgaaatcca tcacttcttc actctgtgct gcgcattccg cagaatatta tccgcttgca
    20041 gccgttgcat ttttcttacg aaaaaggccg cagagtttta tccctgcggc ctgatccggg
    20101 cgcaaattgc cattacggca gcctgacgcc cgtctgacac gttacttctg cttgttcagc
    20161 cagttcacga tcatgctggc aagaatgcca gcaatgaccg gagccgctaa atcgtgccag
    20221 aaggccatgc ccagctctgc gagcgtcata tagccgcctc tgttgtaatg acaacgtttc
    20281 gcggctattc ttgagttgtg taggttcaag aatggccccc gtgatgttgt caggtgcata
    20341 cctgcaacgt gcgggggttt tctctctcca gcaaccaatg ccaccaggga taaagccccc
    20401 gcaacattgc gcctcaccgg aacctttccg gcttgccgct gattctacga ttgcttttta
    20461 ccgtcagcac ccggcaatct tcctcattgc gcgatgcctt ccagcttaat ttctttattt
    20521 gcaatagttg cagttcatat ccgagctgac tccagactgg tagtgaaatg cgcaattttg
    20581 tagaattgcg cctcctcgcg acctccccag tgaatctgtt gcctcatttt ttacatactg
    20641 cgtattcgac ttctccacct gttgcgcaag agaaactggg tttattcatt tttgcgaggc
    20701 cgacttcttt ctggacagga ctttatgcag cacaacacac taccgaaaca caatcagaaa
    20761 ttgccgttta cacgctacga cttcggctgg gttttattat gcataggcat ggcgattggt
    20821 gccggaaccg tgctgatgcc agtacaaatt ggcttgaagg gaatttgggt atttattacc
    20881 gcagcgatca ttgcttatcc tgccacctgg gtagtgcagg acatttattt aaaaaccctt
    20941 tctgaaagcg attcctgtaa tgactacacc gatattatca gtcattacct ggggaagaac
    21001 tggggaattt tcctcggggt tatctacttt ttgatgatta tccacgggat ttttatctac
    21061 tctctctccg tggttttcga cagcgcctcg tacctgaaaa ccttcggttt aaccgatgcc
    21121 gatctttcac aatctctact ttataaagtc gccattttcg ccgtactggt ggcaattgcg
    21181 tctggtggtg aacgattact gtttaagatt tccgggccaa tggtggtggt caaagtaggg
    21241 attatcgtcg tgttcggttt tgcgatgatc ccgcactgga atttcgccaa tataaccgcc
    21301 ttcccgcaag cctccgtctt tttccgcgat gtcctgctta ccattccctt ttgcttcttt
    21361 tctgcggtat ttattcaggt acttaaccca atgaatattg cctatcgtaa acgggaagcg
    21421 gataaagtac tggcaacccg gctcgcgctg cgtacccacc gaattagtta tatcacgctc
    21481 attgcggtga tcctgttttt tgccttttcg tttaccttct caattagcca cgaagaagcc
    21541 gtttctgcct ttgaacaaaa tatctcggca ctggcactgg ccgcgcaggt gatccctggg
    21601 catatcattc atatcacctc tacggtgctg aatatctttg ccgtactgac cgcattcttt
    21661 ggcatttatc tcggtttcca cgaggccatt aaaggcatta ttctcaatct gttaagccga
    21721 attattgata ccaagaaaat taactcacgc atgctgactc tggcgatctg cgcttttatc
    21781 gtcattacgt tgacgatttg ggtttcgttt cgtgtatcgg tgctggtgtt ctttcagttg
    21841 ggaagcccgt tatatggcat tgtgtcgtgc ctcattccgt ttttcctgat ctataaagtc
    21901 gcacaactgg aaaaacttcg cgggtttaaa gcctggctga ttctgctgta cggcattttg
    21961 ttatgcttgt cgccactgtt gaagctgatt gagtaaaccg gagcgcatgg ccccggtttt
    22021 gtgagttacc gctgcggatt ttcatcctga tcaacagcaa aacaagctac cagttgaccg
    22081 ccgtagtctt ttagctgcgg ctgcaactgg gtgcaggggc cgaagcgccg acgacagcgg
    22141 gcgttaaacg cgcagcccgg cggtggattg agcgggcttg gcagttcacc ggtgagcttg
    22201 atgcgctcgc ggcgatcgtc cgggttcagg cgcggcgtcg cggagagtag cgcctgagtg
    22261 tacggatgac gcggattatt gaagatttgg tccttcgttc ccttctccac gcagcggccc
    22321 aggtacatca ccatcacttc atcggcaatg tgctcaacca ctgacaggtc gtgcgagata
    22381 aagacataag acagccccaa ctcctgctgc aaatccatca tcagattcag cacctgcgca
    22441 cgcactgaaa cgtccagcgc agaaaccggt tcatcggcaa tcaccacatc cgggtcgagc
    22501 atcagaccac gggcaatggc gatacgctga cgctgaccgc cggagaacat atgcggatag
    22561 cggtcgtaat gctcggtttt caggccgact ttcgccatca tcgacagggc tttttcccga
    22621 cgctgttctt tgcttaagct ggtattaata agcagcggct cttcaagaat ttgcccaact
    22681 tttttacgcg gatttagcga accataggga ttctgaaaga cgatctggat tttctgccga
    22741 cgcaacttct gcgcctgcgg atcgtgcttg agcagatcct gcccctggta atacagctca
    22801 ccaccggtgg gcatttcgat catcgtcagc aaccgaccga gggtcgattt accgcagcca
    22861 gattcaccta ctactgccag cgttttgcca cgttcaaggt taaacgaaac gccgtccagc
    22921 gccttcacca gacgttccgg cgcgaacata cctttcttca ccggataatg ttttttcagg
    22981 tcgatagcct gcaacagcgg ttgttgcgag gtggcctcat gcgtactcat agtgtcggcc
    23041 tcccggcatc atcaagtggg taatggcatt tggactgacg cccgtcagcg agcatattca
    23101 gcgccggttc ttcagcgcga catctgtcag tggcataggg gcagcgcggg ttaagcaggc
    23161 agccgttcgg gcggtcatac ttgccgggaa caacacctgg caacgacgcc agacgttctt
    23221 tgtcctgagc aaattccggc agcgcacgca gcaatgcctg agtatatgga tgacgcggcg
    23281 catggaagat ggcgtgggca tcgcccgttt ccaccacctg gcctgcatac atcacgatga
    23341 ttttatgtgc cgcttccgcc accagcgcca ggtcatgggt aattaacacc agcgccatgt
    23401 tctctttctg ctgtagctcc agcagtagtt caatgatttg cgcctgaatg gtcacgtcca
    23461 gcgcggtggt cggctcatcg gcaatcagca gctttggccg acaggcaatc gccatggcga
    23521 tcatcacgcg ctggctcatg ccgccggaaa gctgatgcgg gtaaacatcc agacgcgaag
    23581 ccggatcggg aataccgacc tgattcagca ggtcaatcgc ccgctgacgg cgggtacttt
    23641 tgttgccgcc ctgatgcacc ttaatcgctt ccataatctg gaaacccacg gtgtagcacg
    23701 ggttaaggct ggtcatcggg tcctggaaga tcatcgccac ttcggcaccc accaggttgc
    23761 ggcgctcttt ttctgagata cgctgtaaat cctgaccgtt aaactccagc ttttccgcca
    23821 ttacgcggcc cggataatca atcagcccca taatcgccag cgaactgacc gatttaccag
    23881 agccggactc acccacaatc ccgaccactt cgccctgttt tacgctgtag ctgatgcggt
    23941 ctacggcgcg gaacggtgcg ctttcgtcgc cgaaatgcac cgataattta tctacattta
    24001 ataacgccat ctcgaacctc ttactgcttc agtttgggat cgagcgcgtc acgcagaccg
    24061 tcacccatca ggttaaatgc cagcaccgtc agcaggatcg ccagacccgg gaaggtcacg
    24121 acccaccagg cgctttgcgc gaactgcaac acgtcggaga gcatggtgcc ccactccggc
    24181 gttggcggct gcgcccccat gccgaggaaa ccaagagcag ccatatcgag aatggcgtta
    24241 gagaaaccga gcgacgcctg aacaatcaga ggcgcaaggc agttcgggaa gatgttaata
    24301 aacatctgac gcatcgcccc ggcacctgcc acgcgagacg cggtaacgta atcgcgattc
    24361 acctccacca gcacggcggc gcgggttaag cgcacatagt gcggcaaggc aacgaaggtc
    24421 aatgccagcg cggcgttacc aatcgacggg ccgaaaattg ccaccagcac cagtgccaac
    24481 agcaaacttg gcagcgccag catgatatcg accacgcgca tgatgatgtt atcgaccagg
    24541 cctccgaagt aaccggcgat caggccgaga ataacgccca taatcagcga taacacaact
    24601 accagacagc caaccagcag tgacaatcgc gcaccgtaca tcagacgcga cagcacatca
    24661 cggcctacgt catcggtgcc cagcaagtgc gccatgctgc cgccttcctg ccaggctggc
    24721 ggggcgagca gtgcatcgcg gaactgttcc gccgggttat agggtgcaat ccagttggca
    24781 aagatcgcga taaacagcac gatgacgacg taaaccagcc cgacgaccgc gcctttgttg
    24841 cgtttaaaat agtgccagaa ctcctgtaac ggggtcatcg gcaccggtgc gctaatcact
    24901 ttattttcag taacctgtga catgatgtcc ccttacttct tatgacgaat acgcgggttc
    24961 accacgccgt acagcagatc gaccagcaag ttgacgagga taatcatcgt cgccaccagc
    25021 aatacgccgc cctgcactac cggatagtcg cggcgttgca gtgcgtcaat caaccagcgc
    25081 cccagaccgg gccacgagaa gatggtttcg gtcagaatcg cccccgccag caatgttccc
    25141 acctggaggc cgataacggt cactaccggc agcatcgcgt tacgcagcgc atggacgata
    25201 atcacccgca tgcgggtcag ccctttggcg cgcgcggtgc ggatataatc ttcacctagc
    25261 acttccagca tcgaggagcg tgtcatacgc acaatgaccg ccagcgggat agtacccagc
    25321 acaatggcgg gcaagatcat atgggcgacg gcatcgataa agttaccgtc ttcaccccag
    25381 atggcggtgt cgatcagcat aaaaccggtt aacggattgg aatcatcgag gaacaccatg
    25441 tcgctcacgc gaccggagac gggcgtcagg ttccagtgca ccgaaaccag catgatcagc
    25501 atcatgcccc accagaagat cggcatcgag tagccggtca gcgccagacc aaccgccgtg
    25561 tgatcgaaaa tggaaccgcg tttaaccgca gccagcacac cgactggaat accgaccgca
    25621 gtcgcaaaaa tcatcgcgca gacaccaagc tccagcgtgg cctgaaagcg cggcacgaac
    25681 tcttcccaaa ccgggatgcg gcttttcatc gaaatgccta aatcgccgtg cattacgctc
    25741 caaatgtaat tgagatactg ctgccacatc ggtttatcta agccgagttc agccagcagc
    25801 tgcgcgtgac gctctgggga gatcccacgt tcgcccgcca tgatcatcac cggatcgccc
    25861 gggatcatgt ggacaaaggc aaatgtgaga agggtaatac cgataaacgt ggggatgacg
    25921 agtcccaaac gtcggagaat aaactgcaac ataacccgga ttctctgtag tgacccgcag
    25981 cctggaactg cgtgtctgta ttgctcacaa atcaattccc gcccaggtcg ggcagggaat
    26041 gaagtattgc tgccggatgg cgctgcacgc agaggcacag cgccaccggg tatggctttt
    26101 aattattcga tagagacgtt ttcgaagtga tgtttgccta atggatcaac cacatagcct
    26161 ttaacttctt tgcgtaccgg ttcaaacacg gtggagtgag cgatgatcag tgccggagcc
    26221 tgatcgtgca tcaccacctg cgcttgcttg tacagttcaa cgcgtttgtt atggtcgtcg
    26281 gtagcacgcg ccggttgaat cagatcttca aacggtttgt agcaccattt tgagtagttg
    26341 gagccttgtt cagaggcggc gcagctgaac agggtggcga agaagttatc cggatcccca
    26401 ttatcgccag tccagcccat cattaccgtc tggtgctcgc catctttcgc acgcttgagg
    26461 tactcacccc attcgtaggt gacaattttg gcctgcacgc cgactttcgc ccagtctgcc
    26521 tgaatcatct ccgccatgcg gcgagcgttc gggttatacg gacgttgtac cggcatcgcc
    26581 cacaggtcga tggagaaacc tttttccaga cccgcttctt tcagcaaggc tttcgctttt
    26641 tcaggatcgt aggtgtagtc ctgaacgtcg tcgttatagc cccacatggt tggcgggatc
    26701 aggtttttcg ctgccacgcc cgcgccctga taaaccgctt tgatgatagc gtctttgttc
    26761 accgcgtagg tcagagcctg gcgaactttc acgtcatcaa gtggtttttt ctgcacgtta
    26821 tatgaaagat agccgacgtt aagccccggc atttccatca gattgatgga tttatcctgc
    26881 ttcatgcgag cgatatctgc cgggttcggg tacggcatca cctggcattc attcttctgc
    26941 aatttcgcgt aacgcacgga agcatcaggg gtaatagaga aaaccagcgt atcgatctgc
    27001 ggtttggtgc cccagtagcc atcaaacgct ttgtagcgga tacgggaatc tttttggtac
    27061 tgctgtaact ggaacggacc ggtgccgatt gggttgaggt ccagtttttc cggtgtaccg
    27121 gctttcatca tcgcgtcagc atattctttt gacagaatag aggcgaagtc cattgccagg
    27181 tcagcgagga acggcgcttc cgggcgagtc agcacaaact gaacggtgtt gtcgtccacc
    27241 tttttcactt cgctgatcag ctccggcaag cccatgcctt caaagtattc gtagctgccg
    27301 ccagaaactt tatggtacgg gttttgcgcg tttttctgac gatcgaacga gaacaccacg
    27361 tcatcggcgt tcagttcgcg cgtcggtttg aattctttgt tgtcgtgcca cttcacacct
    27421 ttacgcagat ggaaggtata ggttttaccg tcttcgctga cttcccactt ttcagcgagg
    27481 cccgggatta cttcggtggt gccgatttta aattcaacca ggcggttata aagcggcacg
    27541 gaagaggcgt cataggtggt accggaggta aacagctgcg ggttaaaccc ttccggagat
    27601 ccttctgagc aataaaccag agttttagcc tgaacacttg ctgcgacggt catagccacc
    27661 aggctgagac caagcttcag catccctgac tttttcaagg aaatacgcat tattctgctc
    27721 caattgtgat gtttgttgtt ttaacccttt gcagtgggtt gtcgctgcct gacctttttt
    27781 gttttttgcc cggtcgggtc aacgttatga ggtggggatg ccgtactaat taacatcagt
    27841 gtaacagtgc ggatcctcca taaaatgccc ctcgtgacct acaatctgtc aacagaatgt
    27901 gaaaacgtca atacagccaa ccgggattta caccaacggt gagaatccac aaacaaagat
    27961 taaaaaaact tcaaacagct atttgcagca gagaaatttg tgctactcca acatgaccag
    28021 aacaatcagc ttaatattta gcaacaattg gtgattaagt tttatgcatt tagtgaaaaa
    28081 aatctgagga aaaggtggat atctgagcga ttaataccat gaacgctaaa acgcacagcg
    28141 gaaaatgcca gcgccagtca taagtaacgc aaaaaaagat ttaagcaaat ataaaaaaag
    28201 acaatggttt ctgtcacaaa tccgttaatg gatagtgaga tatggggcgc aattttggca
    28261 gggaaatgtg tggtgtgtag gtcgaataag gtgttcacgc cgcatccgac aataagtgcg
    28321 gtgccgttgc ctgatgcgac gctg
//
LOCUS       DAYPHN010000048        28196 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000048 DAYPHN010000000
VERSION     DAYPHN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.048"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(230..1879)
                     /gene="pgi"
                     /locus_tag="ACG0BW_002018"
     CDS             complement(230..1879)
                     /gene="pgi"
                     /locus_tag="ACG0BW_002018"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFK7258984.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNSETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKIVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2116..2312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2404..3753
                     /gene="lysC"
                     /locus_tag="ACG0BW_002019"
     CDS             2404..3753
                     /gene="lysC"
                     /locus_tag="ACG0BW_002019"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFK7258985.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4005..4277
                     /locus_tag="ACG0BW_002020"
     CDS             4005..4277
                     /locus_tag="ACG0BW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFK7258986.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4278..5150)
                     /gene="rluF"
                     /locus_tag="ACG0BW_002021"
     CDS             complement(4278..5150)
                     /gene="rluF"
                     /locus_tag="ACG0BW_002021"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFK7258987.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEYFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5498..6445
                     /locus_tag="ACG0BW_002022"
     CDS             5498..6445
                     /locus_tag="ACG0BW_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFK7258988.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6515..7321
                     /locus_tag="ACG0BW_002023"
     CDS             6515..7321
                     /locus_tag="ACG0BW_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFK7258989.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7331..7738
                     /locus_tag="ACG0BW_002024"
     CDS             7331..7738
                     /locus_tag="ACG0BW_002024"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFK7258990.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDAHVEAVEFVPGENANDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWRQVATAEEDF"
     gene            7738..8232
                     /locus_tag="ACG0BW_002025"
     CDS             7738..8232
                     /locus_tag="ACG0BW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFK7258991.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8298..9095
                     /locus_tag="ACG0BW_002026"
     CDS             8298..9095
                     /locus_tag="ACG0BW_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFK7258992.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9106..9930
                     /locus_tag="ACG0BW_002027"
     CDS             9106..9930
                     /locus_tag="ACG0BW_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFK7258993.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9982..11211
                     /gene="sorE"
                     /locus_tag="ACG0BW_002028"
     CDS             9982..11211
                     /gene="sorE"
                     /locus_tag="ACG0BW_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFK7258994.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11593..12282
                     /gene="pepE"
                     /locus_tag="ACG0BW_002029"
     CDS             11593..12282
                     /gene="pepE"
                     /locus_tag="ACG0BW_002029"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFK7258995.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12372..14003)
                     /locus_tag="ACG0BW_002030"
     CDS             complement(12372..14003)
                     /locus_tag="ACG0BW_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFK7258996.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLTWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14223..17906)
                     /gene="metH"
                     /locus_tag="ACG0BW_002031"
     CDS             complement(14223..17906)
                     /gene="metH"
                     /locus_tag="ACG0BW_002031"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFK7258997.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RLQAERPIQVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18106..18930
                     /gene="iclR"
                     /locus_tag="ACG0BW_002032"
     CDS             18106..18930
                     /gene="iclR"
                     /locus_tag="ACG0BW_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFK7258998.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            19247..21433
                     /gene="espL4"
                     /locus_tag="ACG0BW_002033"
     CDS             19247..21433
                     /gene="espL4"
                     /locus_tag="ACG0BW_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFK7258999.1"
                     /translation="MITRIPRSSFSANINNTARTNEHQTLSELFYKELEDKFSGKDLA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQTDKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDALDKQKLNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHKDDYSSVEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGL
                     VMKAYFDKMASNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMTQLQKMQELHRTEMLTAYNSINLPGLYLAINY
                     GNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSIL
                     NVLPKLAATHHLDNEQVYKFLSAKNRTSSHVLYHVMANGDADMLKIVLDALPLLIRTC
                     HLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPCLAQEINISASDIVDL
                     LTAKSLARDTGLFMAMQRGHMNIIKTIFNVLPTLFNTFKFDKKNMKPLLLANNSNEYP
                     GLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELDH
                     RVIAELILNTINKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(21402..23138)
                     /gene="aceK"
                     /locus_tag="ACG0BW_002034"
     CDS             complement(21402..23138)
                     /gene="aceK"
                     /locus_tag="ACG0BW_002034"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFK7259000.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIRDGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(23187..24491)
                     /gene="aceA"
                     /locus_tag="ACG0BW_002035"
     CDS             complement(23187..24491)
                     /gene="aceA"
                     /locus_tag="ACG0BW_002035"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFK7259001.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(24521..26122)
                     /gene="aceB"
                     /locus_tag="ACG0BW_002036"
     CDS             complement(24521..26122)
                     /gene="aceB"
                     /locus_tag="ACG0BW_002036"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFK7259002.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(26391..27320)
                     /gene="metA"
                     /locus_tag="ACG0BW_002037"
     CDS             complement(26391..27320)
                     /gene="metA"
                     /locus_tag="ACG0BW_002037"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFK7259003.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            27477..27920
                     /locus_tag="ACG0BW_002038"
     CDS             27477..27920
                     /locus_tag="ACG0BW_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFK7259004.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEM
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         6898 a         7149 c         7198 g         6951 t
ORIGIN      
        1 aaaaatttat tttaggatta acctcacggc atgatttccg ttaaatcaca gacaagggga
       61 tttatctgat aaaaaaatgc cgacataacg tcggcattgt tattaaggtt atttaatcaa
      121 tcgtgctatg tattaattgc cgaatacgat gtacatatcg gcatcggttg ccggatgcgg
      181 cgtgaacgcc ttatccggcc tacatagcgg catcaggcat gaacgatgat taaccgcgcc
      241 acgctttata gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttat
      301 catctttcag ctctggcaga atacggttcg ccagttgttt acccagctcc acgccccact
      361 ggtcgaaggt gaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg
      421 caatcaacgc acccaggctg aacggagtga tttcgcgcag caggatagag ttggtcgggc
      481 ggttaccttc gaacactttg aacggcacca cgtagtcaag ggttgccgga tctttaccct
      541 gatcgcgata ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct
      601 gagcgaagaa gttagacagc agtttctggt gatgatcaga gagcgggtta tgggtgatag
      661 ccggagcgat gaaatcgcac ggtacaattt tggttccctg gtggatcagc tggtagaacg
      721 cgtgctgacc gttagtacct ggttcacccc agataatcgg gccagtctgg taatccacaa
      781 cgttaccgtt acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg
      841 ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga
      901 aattgttgta ccagatgcca atcagcgcca gcagtacagg caggtttttc tcggcaggcg
      961 tggtggagaa atgcttgtcc atcgcgtgtg cgccagaaag cagttcaaca aaattatcaa
     1021 agccgatgga gagaacaatc gacaggccaa tcgctgacca caaagagtaa cgaccgccga
     1081 cccagtccca gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg
     1141 cattggtgga aagcgccgca aagtgtttcg caacgtgttt ttcatcacca gccgctttca
     1201 ggaaccagtc acgcgcacta tgggcgttgg tcatggtttc ctgagtggtg aaggttttag
     1261 aggctaccag gaacaacgtg gtttccgagt ttactttttt cagcacttca gcgatgtgag
     1321 tcccatcgac gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt
     1381 cggtcaccat gtacgggccg aggtcagaac cgccgatccc gatgttcact acgtcagtga
     1441 ttgctttgcc ggtataaccc ttccactcac cggagataat cgcttccgag aaggttttca
     1501 tcttctccag caccgcgttg acttctggca ttacgtcttt gccatcaacc agaatcgggg
     1561 tattgctacg gttacgcagc gctacgtgca gcacggcgcg gttttcagtg cggttgatct
     1621 tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatctt
     1681 gtaatttcgc cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat
     1741 cgtcgaaggt tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga
     1801 tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg
     1861 ttggattgat gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag
     1921 attgtagcgc cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt
     1981 gtaaaccctt agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg
     2041 aaaaatccgc atgaccccat cgttgacaac cgccccgctc accctttatt tataaatgta
     2101 ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc
     2161 cagtcctgtg ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt
     2221 tcatcatcgg ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg
     2281 cgtttcgcaa gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc
     2341 cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta
     2401 gttatgtctg aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc
     2461 atgaaccgca gcgctgatat tgtgctttct gatgccaacg tgcgtttagt tgtcctctcg
     2521 gcttctgctg gtatcactaa tctgctggtc gctttagctg aaggactgga acctggcgag
     2581 cgattcgaaa aactcgacgc aatccgcaat atccagtttg ccattctgga acgtctgcgt
     2641 tacccgaacg ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca
     2701 gaagcggcgg cgctggcaac gtctccggcg ctgacagatg aactggtcag ccacggagag
     2761 ctgatgtcga ccctgctgtt tgttgagatc ctgcgcgaac gcgatgttca ggcacagtgg
     2821 tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta
     2881 gccgcgctgg cggaactggc cgcgctgcag ctgctcccac gcctcaatga aggcttagtg
     2941 atcacccagg gatttatcgg tagcgaaaat aaaggtcgga caacgacgct tggccgtgga
     3001 ggcagcgatt atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc
     3061 tggaccgacg tcccgggtat ctacaccacc gatccacgcg tggtttccgc agcaaagcgc
     3121 attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg
     3181 catccggcaa cgttgctacc cgcagtacgt agcgatatcc cggtctttgt cggctccagc
     3241 aaagacccac gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctattc
     3301 cgcgcgctgg cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg
     3361 cattctcgcg gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta
     3421 gacttaatca ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc
     3481 tccactggcg ataccttgct gacgcaatct ctgctgatgg agctgtctgc actatgccgg
     3541 gtagaagtgg aggaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc
     3601 tgcggcgttg gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt
     3661 tacggcgcat ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg
     3721 gtgcagaagc tgcattttaa tttgtttgag taaacacagt atggcccgga agctatattt
     3781 cgggccgtat tgtttttctt gtcattatgc ttatcaataa acgagcctgt actctgttaa
     3841 ccagcgtctt tatcggagaa taattgcctt taatttttta tctgcatctc taactaatta
     3901 gagaaagaga taaatagtta agagaaggca aaatgaatat tatcagttct gctcgcaaac
     3961 gaattcccgt acactgaact ctctggctaa ctgagggcac cagcatggca ctcccccgta
     4021 ttacccaaaa agagatgact gaacgcgagc agcgtgaact aaaaacattg ctggatcgcg
     4081 cgcggattgc gcatggtcgc gtgttgacta attcggaaac caacagcatt aaaaaagagt
     4141 acatcgataa gctgatggtt gagcgtgaag cagcagcaaa aaaagcccgc caattgaaga
     4201 aaaagcaggc ttataaaccg gatcctgaag cgtcatttag ctggtcagca aatacgtcga
     4261 cgcgcggcag gcgctgatta acgccccttc tttttacgcc ccggcgaggt aaaacgctta
     4321 ccgttggacg ccgggcgacc gcctttttcc gccgtttttt ccatcttaac gactggacgt
     4381 ttgatgccta cagttttcgg tttggccttc gctttaggtt ttgcctcgga agaggaattt
     4441 tcaatgagct taaagaggtc gattaactca tcgtcagtta aatcacgcca ttcccccagc
     4501 ggaatgccgc ttaagctaac gttcatgatg cgcgtacgtt ccagcttttt cacttcatag
     4561 ccaaagtact cgcacatgcg acgaatctga cgattcagcc cctgcaccag ggtaatgcgg
     4621 aagacaaacg gcgcttcttt tttgactttg cactttttcg tcaccgtccc gaggatcggc
     4681 acccccgccc ccataccacg aataaattca tcggtaatcg gtttatcgac cgtcaccaga
     4741 tactctttct catgatcgtt acccgcacgg aggatcttat tcaccagatc gccgtgattg
     4801 gtgaggaaaa tcagcccctg ggagtcttta tccaggcggc caatcgggaa cacgcgtttg
     4861 ctgtggttaa cgaaatcgac aatgttatcg cgctcgccat cttcggtggt gctgacaata
     4921 ccgacgggct tgttcagggc gataagtacc aaatcttcgg cttcccgagg ctcaatcaac
     4981 tgaccgttta ctttcacaac gtcgccgggt ttcacctgat cgccaatggt ggctcgcttg
     5041 ccattaagga acacattgcc ttgctcgata tagcgatccg cttcgcggcg tgagcaaatt
     5101 ccgctttcgc tgatgtattt atttaaacgg actgatgagt cgggcagcat aaattctcct
     5161 gtaaaacgcg gaatataccg tacctcttag cggggaaaaa cagggttatg cggcaacgtc
     5221 gaaattaaat gtgatctgcc acgcctttta tcacaaaagt gtctccggaa gtacgatgca
     5281 aatttgcctg gcaaaataag gataatcaac atattacgaa tcgctgcaca cctgtgctga
     5341 tgactactac gcagagaaac aaatgtgcaa gagaaagcgc tatttctccc catgcaaaga
     5401 gtgaaaaatc gctttgaaag gccatcatga tggggttata tcagatcaag cggtgagaaa
     5461 gcgtatttgc acaaatgtgc agaaccagga ggcggcaatg gaaaacagtg acgatatccg
     5521 tttgattgtg aagattgccc aactctatta cgaacaggat atgacgcagg cgcaaatcgc
     5581 gcgcgaactg ggtatttacc gcaccaccat cagccgcttg cttaaacgag gccgcgatca
     5641 gggaattgtc accatcgcca tcaactatga ctacaacgaa aatctctggc tggagcagca
     5701 actgaagcaa aagtttggcc tgaaagacgt tgtggtggtg tcgggaaatg atgaggatga
     5761 agagactcaa ctggcgatga tggggttaca cggcgcgcaa ctgctggatc gcttgctgga
     5821 acctggcgat attgtcggtt tttcctgggg tcgcgcggtg agcgcactgg ttgaaaactt
     5881 gccgcaggcg gggcaatcgc ggcagttaat ctgcgtacca attattggcg gaccgtccgg
     5941 taaactcgaa agccgctatc atgtaaacac attaacctac agcgcggcag cgaagctgaa
     6001 aggggaatcg catctcgcgg attttccggc tctgctggat aacccattaa ttcgtaatgg
     6061 gatcatgcag tctcagcact ttaaaaccat ctctgcctac tgggataatc tggatgtcgc
     6121 cctggtggga attggctcac cggccattcg cgacggcgct aactggcatg cgttttatgg
     6181 tggtgaagag agtgacgacc tgaatgcccg ccaggttgct ggcgatattt gctcgcgctt
     6241 ttttgatatt cacggcgcaa tggttgaaac gaatatgagt gaaaaaacac tctctatcga
     6301 aatgaataaa ttaaagcagg cacgatattc cattggcatt gccatgagtg aagaaaaata
     6361 cagcggaata gttggtgcac tgcgtggaaa atatattaat tgtctggtaa cgaatagcag
     6421 cacagctgaa ctattactga aataaaaatc ttcccgatgt ttaccgtaat aaaaattacg
     6481 gcgggtccct attatctaaa aacaggagtc tgttatgcaa acgtggttaa atttgcagga
     6541 taaaatcatt atcgtcaccg gcggcgcatc cggtataggt ctggcgattg tagaggaatt
     6601 attagcacaa ggcgcgaatg tacagatggt cgatattcac ggtggcgatg gacaatatga
     6661 aggtcataaa ggctatcagt tttggccgac cgatatttcc agcgccaaag aggtaaatca
     6721 tacggtagca gaaattatcc agcgttttgg tcgcatcgac ggtctggtta acaacgccgg
     6781 ggtcaatttt ccgcgtctgc tggtcgatga gaaagcgcct gccgggcagt atgaactcaa
     6841 cgaagctgca ttcgaaaaaa tggtcaatat caaccagaaa ggcgtttttc tgatgtcgca
     6901 ggcggtggcg cgacagatgg tcaaacaaca cgatggcgtg attgtgaatg tttcctcaga
     6961 aagtgggctg gaaggctcag aaggccaaag ctgttacgcc gcgaccaaag ccgcgcttaa
     7021 tagcttcacg cgatcctggt cgaaagaatt gggtaaacac ggtatccgtg tagtcggtat
     7081 cgcgccgggg attctggaaa aaaccgggct acgcacgccg gaatatgaag aagcgctggc
     7141 gtggacgcga aatatcaccg tcgagcagct gcgtgaaggc tataccaaaa acgccattcc
     7201 tattgggcgc gccggaagat tagcagaagt cgctgatttt gtttgttatc tgttgtctga
     7261 acgcgccagc tatatcaccg gagtaaccac taacattgcg ggcggcaaaa cgcgcggcta
     7321 aggaggcttt atggtcaacg ccattttttg tgcccacggc aaattagctt gcgccatgct
     7381 ggaatcggtt cagatggttt acggcgacgc ccatgttgag gcagtggaat ttgtacccgg
     7441 tgagaacgcc aatgacattg tggcaaagct ggaaaagtta gtgagcattc acaatcaaga
     7501 cgaatggctg attgccgtgg acttacagtg cggcagcccg tggaatgcgg cagcgatgct
     7561 tgcgatgcgc aatcctcgtc tgcgggtaat cagcggcctt tctctgccgc tggcgctcga
     7621 actggttgat aaccaggaca gcatgaatgt tgatgaactg tgtgaacacc tgacgcaaat
     7681 cgcaaagcaa tcctgcgtcg tctggagaca ggtggcaacg gcagaggagg atttctgatg
     7741 aacattacgc tcgcccgcat tgatgaccgc ctgatccacg gtcaggtcac taccgtctgg
     7801 tcgaaagtgg ctaacgccca gcgcattatt atctgcaatg acgaagttta taacgatgaa
     7861 gttcgccgga cattattgcg tcaggctgcg ccaccgggaa tgaaggttaa cgtggtcaat
     7921 attgaaaaag ccgtcgcggt ttatcacaac ccgcaatatc aggacgaaac cgttttttat
     7981 cttttcactc gcccacagga tgccttagcg atggttcgcc agggggtaaa aatagacacc
     8041 ctgaatattg gcggcatggc ctggcggccc ggtaaaaaac agttaaccaa agcggtttca
     8101 ttagatgatg atgatattaa cgcatttcat gagttaaata atcttggcgt catactggat
     8161 ttacgcgtcg tagcgtcaga tccctcaatc aatattatcg acaaaataaa cgaacagtta
     8221 atcgcaaatt aaataatagc gcctgtgtac atatgcttta acaggcaggg atgacgacac
     8281 ttaaaaggtg acaaatcatg gaaataagta ccctacaaat cattgctata tttctttttt
     8341 cctgtattgc cggaatgggc agcgtgctgg atgaatttca gactcatcgc ccgttaattg
     8401 cctgtacggt gattggttta attctcggtg atttaaaaac cggaataata ctcggtggta
     8461 cgctggaatt gatagctctc ggctggatga acgtcggcgc ggcgcaatct ccggattctg
     8521 cactcgccag cataatctcc gccattctgg ttatcgttgg tcagcaaagc atcgccaccg
     8581 gaatcgccat cgcgttgcct gtggctgcgg caggccaggt gctgacggtg tttgcccgta
     8641 ccatcaccgt agtgttccag cacgcggcgg ataaagcagc ggaagaggcg cggtttcgca
     8701 ctctcgatat tctgcatgtc tccgcgcttg gcgtgcaggc gctgcgcgtt gctattccgg
     8761 cactgattgt ctcgctgttc gtcagcgccg atatggtcag caatatgtta aacgccattc
     8821 ccgaatttgt gacccgagga ctgcaaattg ctggcggttt tatcgtggtg gtcggttacg
     8881 ccatggtgct acgtatgatg ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc
     8941 tcgcaggtgg ctacctcgat ctcagtctgc tggcgttcgg tggcgtcggc gtgatcatgg
     9001 ccctgctcta catccagcta aatccacagt ggcgtaaagc tgaacctcaa ctccaggcca
     9061 ccgcctccac cgcccttgac caacttgacg attaacggag ccatcatgga acagagaaaa
     9121 attacacgca gcgatctggt gagcatgttt ctgcgctcca acctgcaaca ggcgtccttt
     9181 aactttgaac gtattcatgg gctgggtttt tgctacgaca tgatccccgc catcaagcga
     9241 ctttacccat taaaagagga tcaggttgcg gcgctcaggc gacacctggt gttcttcaat
     9301 accacgccag ccgtatgtgg cccggtcatc ggcgtcactg ccgccatgga agaggcgcgg
     9361 gccaacggcg cggaaattga tgacggtacc attaacggca tcaaagtcgg tctgatggga
     9421 ccattggcag gagttggcga tccactggtc tggggaacgc tgcgcccgat taccgccgcg
     9481 ctcggcgcat ctctggcact ttcgggcaac attctcggcc cgcttctgtt tttctttatt
     9541 ttcaatgcgg tgcgtctggc gatgaagtgg tatggcctac agctcggctt tcgcaaaggg
     9601 gtgaatatcg tcagcgatat gggcgggaat gtgctgcaaa aactcaccga aggcgcgtcg
     9661 attctcggac tgtttgtgat gggcgtgctg gtgaccaaat ggacgtcaat caacgtaccg
     9721 ttggtggttt cacaaacgca tgccgccgat ggctccaccg tcaccatgac cgtgcagaac
     9781 attctcgacc aactttgccc tggtttgctg gcgctcggtc tgacgctact gatggttcgt
     9841 ctgctcaaca aaaaaattaa cccggtatgg ctgattttcg ccctgtttgg cttagggatt
     9901 atcggcaatg cgctgggctt cctgtcctga ttcttcgccc cggcacgact gccggggcca
     9961 tcgctcaaca tgaggtggtt tatgaaaaca acagctctgc gtctttatgg taaacgtgat
    10021 ttacgcctgg aaacctttga tcttcctgaa atgcaggagg atgaaatcct cgcgacggtg
    10081 gtcaccgaca gcctgtgcct ctcttcctgg aaagaggcca atctgggtga aaaccataaa
    10141 aaagtacccg acgatgtggc gaccaacccc atcatcatcg gccacgagtt ttgcggcgat
    10201 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    10261 caggccaacc tgcaattgcc ggatcgcccg gactgccccg gctactcctt cccgtgggtg
    10321 ggcggtgaag ccacacatgt ggttatccca aatgaggtta tggaacaaga ttgcctgctg
    10381 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    10441 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    10501 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggact gttggcgatt
    10561 gattatgcgc tgcatggacc cgttaacccg tcgctgctcg tcattaccga taccgacaac
    10621 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    10681 ctcaatgccg ccgatgcagc atttgatacg ttaatggcac tgagtggcgg tcacggcttc
    10741 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    10801 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    10861 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    10921 accgatgaca tgcgcgcagc agtcaaattg attgaagaga aaaaagtgca ggccgcaaaa
    10981 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    11041 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgcttat gtcactcacg
    11101 cagattcagg atcaagcact ggcggcaatt ctggcgcgtc atcaggggat ctggtccggt
    11161 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    11221 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgaccggat gtgcggctga
    11281 ttgctgaagc agcacaattt gcatgagaag gataaaaaat atcagcgcct taaggggcgc
    11341 tcaaattggt ggcaaagtac acattgttta tgccggatgc ggaatgaagc cttattcggc
    11401 ctacataatc tcgaaaattc aatatattgc agttatctgg taggactggt aagcgtagcg
    11461 catcaggcaa tgctgtgttt gtcatcagtc tcagcgcccg gtgcaatacg tgacatcgtg
    11521 agccatgtca gtaagtggtt aaattcgcag tggtagcatt ccttccgtgt tttcttacta
    11581 accggagaag taatggaact gcttttattg agtaactcga cgctgccggg taaagcctgg
    11641 ctggaacacg cgctgccgct aattgctgaa cagttgcagg gtcgccgctc agcggtgttt
    11701 atccctttcg ctggcgtaac gcagacctgg gatgattaca cagcgaaaac ggctgcggtt
    11761 ctcgctccgc tgggtgtttc tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg
    11821 attgaaaatg ctgagatcgt gattgtcggc ggcggtaata ctttccagtt gctgaaacag
    11881 tgccgcgagc gcgggctgct ggcaccaatt actgacgtgg ttaaacgtgg cgcgctgtat
    11941 attggctgga gcgctggcgc taaccttgct tgcccaacca ttcgtaccac caacgatatg
    12001 ccgattgtcg atccacaagg tttcgatgcg ctgaatctgt tcccgttgca aatcaacccg
    12061 cacttcacca acgcgctgcc ggaaggccat aaaggtgaaa cgcgtgagca gcgtattcgc
    12121 gaactgctgg tcgtcgcgcc agaactcaca attattggtc taccggaagg taactggatc
    12181 acagtaagta aaggtcacgc tacgctgggt ggcccgaaca ccacttatgt gtttaaggct
    12241 ggtgaagaag cggttccgct ggaagcgggt caccgttttt aagtgaattg ccggatgcat
    12301 gatccggcat cttgtacata gtgcctgatg cgacgcttta cgcgtcttat caggccttca
    12361 ggggcgcagt attaatactc gtcccgtcct tcatcttcat caggctgttc cagcacactg
    12421 taagcgactg aacaaaacag cgagttcaga cgttgcatat ctcccaataa gcctaaatgt
    12481 agcgaactgg tttcaatact ctgcacgttt tgctgatgca ggcgatcgac gtgggcgtgg
    12541 gaatagcggc gattaagaat gcgaaaacga tgtttgctgc ggcgcaaacg acgggcgctg
    12601 gtaacatcgc cagagaagaa aaccgacatt gccagtttta aattactgag caattgctca
    12661 tagagcgcat ccagttcctt caagccatca agcgaaaatg ctcgccttgc agccagcgat
    12721 ttgtcggcaa tttcactacc catgcgctcg acgatatcgg aggcctgttc aaggtttagc
    12781 gacatttcga tgatctccgc ccagcggcgc gactcttctt ctgccagttc ctcttttggc
    12841 attcgcgcca gataaagttt aatcgcggta tagagcacgt tgatatcatc cgccagcttg
    12901 cgcagctctt tctcctgccg tggctcgccg tgcatcactt tattcaaccc ttccatcatc
    12961 tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg cgttcgccag agcaagcgtg
    13021 ggcgtatcca gcgcgctgac atccagatgt ttaggccgta gctgggtatc cagttccggt
    13081 tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc
    13141 aggcaacgta caaggttgta gaagacgtgg aaatagatca ccagttccgc ttttggcaat
    13201 gacaacttcc ccattgtctc tgccagcaaa tggacaaacg gcaggataat caggctaccc
    13261 accagcttaa acagcagact acccagcgcg acacggcggg ctgcggcatt ggcggcactg
    13321 ttgttgagca tcgccagcag gccggaaccg aggttagcac caatcaccag acagagcgcc
    13381 acggggaagg agataatgcc tgcagcggtc agagtcgcgg tcagcagtac agcagcaagg
    13441 ctggagtagc tgataatggc gaacatcgcg ccaatcagcg catccagcag aatatcgccg
    13501 gtcagcgagg caaagatcac ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc
    13561 acaatcaact ccagcgccag caaaatcagc ccaagaccaa taccgacgcg gcccagttgc
    13621 ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc cgataaaaat aagtaatggt
    13681 gacagccagg ttaaatcgaa ggtgagaata cgcgccatta gcgccgtccc gacatctgca
    13741 cccagcacaa tcaccagagc cggtgcgagg gctaccagat cctgagcgac gaacgaggtc
    13801 accagcatgg tggtggcatt actactctgc accagtgcgg taacgccgat ccccgcgcaa
    13861 aaggcaagcg gctttttttc gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag
    13921 acgcgcatta cgccggttcg aacaatatgc gtcccccaga ccagcagggc gacggcagaa
    13981 agcaggtgaa gcagcgttaa cacgagttca ggttctcctt atcgttatat gttcctgaag
    14041 caattgaatg accaaaggac cggaaacacg ctgactggca tctttactgt gccttttgac
    14101 cttcgttttt ccggctaaga ttattccagc ctgattccag tataagtgtt tcatattaat
    14161 aaagagacag ggcactcatt gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg
    14221 aatcagtccg cgtcataccc cagattcggt gccagccagc gctcaacatc tgagacgctc
    14281 atacctttac ggcgggcata gtcttccacc tgatcgcgct ggatttgcgc caccgcgtaa
    14341 tacttgctgt ccgggtggct gaagtaccaa cccgaaaccg atgcaccagg ccacatggcg
    14401 aaagattctg tgagtttcat gccagtgtgt ttttccactt ccagcagctc ccagatggtg
    14461 gctttttccg tatgttccgg gcaggccgga tagcccggtg ccggacggat gccctggtag
    14521 ttttcgcgga tcagctcttc gttgctgagg ttctcgttcg gcgcatagcc ccagtagact
    14581 tttcgcaccc gctcatggag atactccgca aaggcttccg ccagacggtc agcaagggct
    14641 ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc
    14701 tcttccagac cgccagtcac ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc
    14761 ggcgcaacga agtcagcgag acagtagtta gcgaagccgg ttttttcggt ctgttgacgc
    14821 agatggtggc tgacgttgat cacatgggta cgcgtttcgt cacggtagat ttcaatgtca
    14881 tcgcccacac ggtttgccgg gaacaggccc accacgccac gcggattcag cgttttttcg
    14941 gcgcttaatt tatccagcat gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc
    15001 accacttcat cttccagaat gcgcggatac ttcccggcca gcgaccaggt cataaagaac
    15061 ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg cttcgacttc ctgcacgccg
    15121 agacggtgcg ccaccggcgg cgtataagcc tgccagtcaa aagcgaagtc gttatcacgc
    15181 gccgcttcca gcgtgaccgg tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg
    15241 gtttcgtact ccttgcgggt acgagcgaca aaatcatcac gctgagtatc ggaaagcagc
    15301 gccgccacca caccaacggt gcgcgatgcg ttctgtacat acaccgtcgg gccgctgtag
    15361 ttctgctcaa ttttcaccgc cgtgtgcgct tttgaggtgg tcgcgccgcc aatcagtaac
    15421 ggaatggtga agccctgacg ctccatctct ttcgccacgt taaccatctc gtccagcgac
    15481 ggcgtgatca atcctgaaag gccaatcaaa tcagcattca cttctttggc ggtacggaga
    15541 attttttccg caggcaccat cacgccgaga tcgacaattt cgtagttgtt acattgcagc
    15601 accacaccaa cgatattttt accgatgtcg tggacgtcgc ccttcacggt ggcgatcacc
    15661 atcttgccgt tggttttgcc ctgctctttg ctggcttcaa taaacggttc gaggtaggcc
    15721 accgcctgct tcatcacacg cgccgatttg accacctgtg gcaggaacat tttcccttcg
    15781 ccaaacagat cgccgaccac gttcatgccg tccattagcg ggccttcaat cacctgaata
    15841 gggcgctccg cctgcaggcg ggcttcttcg gtatcctgct cgataaactc ggtaatgcct
    15901 ttgaccagcg agtattccag acgtttattc acttcccacg agcgccactc cgcctgctgg
    15961 gcgttagcgg tgtcgtcggt tttgctgccg cgatatttct cggcaagctc cagtaaacgc
    16021 tcagtgccat cgtcgcgacg gttaagaatc acatcttcca ccgcgtcgcg cagttcagcg
    16081 ggtaggtcgt cgtaaatcgc cagctgcccg gcgttgacga tccccatatc catgccattg
    16141 cgaatagcgt agtagaggaa caccgcgtga atggcttcgc gcaccggatc gttaccacgg
    16201 aatgagaaag aaacgttaga tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg
    16261 atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt tgtgctcttc aatgccagtt
    16321 gcgaccgcga agatgttcgg gtcgaagatg atatcttctg gcgggaagcc aacctcttcg
    16381 gtgaggattt tgtacgcccg acggcaaatc tcgattttcc gtgcgcgggt atcggcctgc
    16441 ccctgttcgt caaaggccat taccacaact gccgcaccgt aacgacgcaa caatttcgcg
    16501 tgatggataa aagcgtcgac gccttctttc atcgaaatag agttaacaat gcctttgccc
    16561 tggatacact tcagaccttt ttcaatgaca tcccattttg aggagtcgat cataatcggc
    16621 acgcgagcga tatccggttc accggcaatc agattgagaa aacgcaccat cgccgcttcg
    16681 gcatcgagca tcccttcatc catgttgata tcgataatct gcgcgccgtt ttccacctgc
    16741 tggcgggcca catccagtgc ctcgctgtat ttctcttctt tgatcaggcg cttgaattta
    16801 gcggaaccgg tgacgttggt gcgttcaccc acgttcacaa acaggctatc ttcgccaatg
    16861 ttcagcggct ccaggccgga caaacggcag gctacgggga tttccggcag tttacgcggc
    16921 gctaatcctt ctactgcacg actcatcgcg gcaatatgtt gtggcgtggt gccacagcag
    16981 ccgccgacga tattgagaaa gcccgcttct gcccattcac gtatctgttt tgccatcgtg
    17041 tcggcgtcga gatcgtattc accaaaggcg ttgggcagtc cggcgttcgg gtgcgcggtg
    17101 acgtagcatt ccgcaatccg tgatagttcc tgtacgtact ggcgcagttc atctggcccc
    17161 agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc gcaatgagtt gtaaaatgct
    17221 tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg tgatggtgcc agagatcata
    17281 atcggcagct caacgcccag cgcttcaaac tccgttttca ccgcaaagac cgccgctttg
    17341 gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat ccgcaccacc ttccaccagc
    17401 gctttggtgg attcgcgata ggccgccacc agctggtcaa aagtgatgtt gcggaatgcc
    17461 ggatcgttga cgtccggaga aatagacgcc gtgcggttgg tcgggccgag aacaccggca
    17521 acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcat cagcacaggc gcgcgccagt
    17581 ttcgccgccg caaagttgat ttccgccgac agggattcca tctggtaatc cgccatcgca
    17641 atggttgtgg agttgaaggt gttggtttcg atgatatccg cgcccgcttc aaagtaggcg
    17701 ttgtggatag cggcgatcac ttccggttta ctgagtacca gcaggtcgtt gttgcctttg
    17761 aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag
    17821 ctctggatca tggtgcccat accgccgtcc agcaccagaa tacgttcatt taactgcgca
    17881 cgcagttgtt ccactttgct gctcacactt gctcccgaca cgctcaaccc agatttaata
    17941 aaaattcaac aaaccatact ggcataaacg catctgtggt aaaagcgacc accacgcaac
    18001 atgagatttg ttcaacatta actcatcgga tcagttcagc aactattgca ttagctaaca
    18061 ataaaaatga aaatgatttc cacgatacag aaaaaggaga ctgtcatggt cgcacccatt
    18121 cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac cagcgactgg acaggttcag
    18181 tctttaacgc gtggcctgaa attactggag tggattgccg aatccaatgg cagtgtggca
    18241 ctcacggagc tggcgcaaca agccgggtta cccaattcca cgacccaccg cctgctaacc
    18301 acgatgcaac agcagggctt tgtgcgtcag gttggcgaac ttggacactg ggcgatcggc
    18361 gcgcatgctt ttatggtcgg tagcagcttt cttcagagtc gtaatctgtt agccattgtc
    18421 catcctattc tgcgcaatct gatggaagag tccggcgaaa ccgtgaatat ggcggtgctg
    18481 gatcaaagcg atcatgaagc gattattatc gaccaggtac agtgtaccca cctgatgcga
    18541 atgtccgcgc ctatcggcgg taaattacct atgcacgctt ccggtgcagg taaagccttt
    18601 ttagcccaac tgagcgaaga acaggtgacg aagctgctgc accgcaaagg cttacatgcc
    18661 tatacccacg cgacgctggt gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc
    18721 aaacggggtt attcatttga tgatgaagaa catgccctgg gactgcgttg tctcgcggca
    18781 tgtattttcg atgagcaccg cgaaccgttt gccgcaattt caatttccgg accgatttca
    18841 cgtattaccg atgaccgcgt gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa
    18901 gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg cgggcagagg caatattctg
    18961 cccgtcatac ccgactggca atagaataag ggtgtctgtt aatcgcattg acgccaaaat
    19021 aacttattgt catacacttc acacgctttt attacctcag cattattttt aaacatcaaa
    19081 ccacttaatt attaacaaaa tcgcaagaac tcattgcgcc gaaactttta cagctgcatt
    19141 atgagattga gcgatttatg atatttcttt agttgtaatc atattaaatt ttttcactgt
    19201 tctgatagtt aaaattcaag acatcaataa acaatgagat atttaaatga ttactcgtat
    19261 tcctcgtagt tctttctctg caaatattaa taatacagcc cggacaaatg aacaccaaac
    19321 cctgagtgaa ttgttttata aagaactcga ggataaattt tctggcaagg acctggcgac
    19381 gcctctatta aaaagcttct cagagaactg tagacaaaat ggtcgtcata tctttagtaa
    19441 caaggatttt gtcattaagt tttccacgtc cgtcttacag actgataaaa aagaaattac
    19501 gataattaat aaaaatgaaa acacgacact cactcaaacc attgctccaa tatttgaaga
    19561 atacctgatg gaaattttac ctcaacgctc agacgctctt gataaacaaa aattaaatct
    19621 aaaatcagat agaaaagaaa aagaatttcc aagaattaaa cttaatggtc aatgttattt
    19681 tccggggcga ccccaaaacc gtatagtatg ccgacacatt gctgcacaat atattaatga
    19741 tatttatcag aatgttgact acaaacctca taaagatgat tactcttcag ttgaaaaatt
    19801 tctcactcac ttcaacaaaa aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc
    19861 tgaggggcgt tgcgttgctg cctgcggtga tttcgggcta gttatgaaag catattttga
    19921 caagatggca tcaaatggca tcagtgttat ggcagccata ttattggtgg ataaccatgc
    19981 tttgacggtc cggctaagaa taaagaacac aactgaagga tgtacccatt acgtggtttc
    20041 ggtttatgat ccaaatgtaa ctaacgataa aataagaatc atgagcgaaa gtaaagagga
    20101 tattaaacac tattcattga tggactttat gaatgtagat tatagcctcc tgaaatggtc
    20161 aaatgatcat gttattaacc aatctgttgc aataattcca gcacttccga aagaacagct
    20221 attgatgcta aaaggatctg tggatgaaat aacccctcca ttatcacctg caacgatgaa
    20281 tttgctaatg gcaattggtc agaatcatca acttacgcaa ctgatgactc aactccagaa
    20341 aatgcaagaa ctacatagaa cagaaatgtt gactgcctat aatagtatta acctccccgg
    20401 tttatatttg gctataaatt atggtaatgc ggatatcgtt gagactattt tcaattcatt
    20461 gtcagagcca ggatatgaag ggttactctc gaaaaaaaat ctcatgcata ttctggaggc
    20521 aaaagataaa aatggttttt ctggattatt tttagcgata tcacgtaagg ataaaaatgt
    20581 tgtaacctcg attctgaacg tcttacctaa actggccgca acacatcatt tagataatga
    20641 acaggtgtat aaattcctga gtgccaaaaa tagaacgtcc agccatgttt tataccatgt
    20701 tatggcgaat ggtgatgccg acatgctgaa aattgttttg gacgcgttac ctttgttaat
    20761 tcgcacatgt catttgacta aagaacaggt actcgatctc ctgaaggcaa aggattttta
    20821 tggttgccca ggactatacc tggcgatgca aaatggacat agcgatatcg tgaaagttat
    20881 tctcgaagca ttaccctgtc tggcccagga aattaacatt tcagcttccg atattgtcga
    20941 tcttctgacc gctaaaagtc ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg
    21001 acatatgaac attattaaga ctatttttaa cgtattaccc actctgttta atacgtttaa
    21061 attcgataaa aaaaatatga agcccctcct cctggcaaat aactctaatg aatatcccgg
    21121 tttgttttca gcgatacagc ataaacaaca aaacgttgta gagacggttt atcttgcttt
    21181 atctgaccat gcacgcctgt ttggatttac tgctgaagat attatggatt tttggcaaca
    21241 caaagcgcca caaaaatact ctgcctttga gttggctttt gaattggatc accgggttat
    21301 tgctgaatta atccttaata cgataaataa gatggctgaa agctttggct ttacggataa
    21361 ccctcgatac attgcggaga aaaattatat ggaagcttta ctcaaaaaag catctcccca
    21421 taccgtacgc taaatctttg cctgcgccga tacgcataaa catcttccac atgcccgtca
    21481 cggatacggt tttgtagcgc gcgccagtaa tcagcgcgga acaggtcggc gtgcatctct
    21541 tcaaagagtg gcccaatgcg ggggtcagcg catagccagt ggcgaaactc ttccgggaaa
    21601 acatcgcccg gcgagacgct gtaccacggt tcgctggcaa gttcgtcctc cgggtagcgc
    21661 ggcggcggga tgtcgcggaa gttcacttcc gtcatgtagc aaatttcatc gtaatcataa
    21721 aacaccacac gcccgtgacg ggtgacaccg aagtttttaa acagcatgtc gccagggaaa
    21781 atgttagcag cggcaagctg gcgaatagcg ttaccgtatt cttcaatggc atcgcgcaac
    21841 tgctgacctt ccacttgctc cagccagata ttgagcggca ccatccgccg ctcaatataa
    21901 agatggcgaa tcacaatttg ttcgccgaga tcggtgattt tttccgctgc ttcctggagc
    21961 agtaatgcca ttaatgccgg ggaaatatgc cgcttctcca gcacaaagtt ttcaaactcc
    22021 tgggtgtccg ccattcggcc cacgcgatcg tgctctttca ccaattgata gcaggcacga
    22081 acgtgagcgg cagacatctc tttctgcggc gcgaacttgt ctttgatgac tttgaatact
    22141 cgatcaaaac ccggcaacgt aaacaccaac atcaccattc cacgaatacc cggcgcttca
    22201 atgaactgct cattacagcc ctgtagataa acgagatatt cgcggtagct ctcggttttg
    22261 gcatgcttct ggcagccgat agccatatac aattcagcgg tggttttacc tggcagaatt
    22321 tcccgtagcc actcgaccag tgctgcgggc agcggcgcgt aaaccataaa ataagaacgc
    22381 gcaaagccaa aaacaatgct cgcttcggcg gtcgtcgtca ggcaggtatc aataaataac
    22441 tcgccgtcgt ccgtctggtg gatcggcagc aaaaatggca atgtgccgga aggcgtgatc
    22501 agtttgccta ccagccaggc ggctttattg cggtaaaaca gttcgttcgc cacctgtaaa
    22561 tgactttccg cgaggttgtc tgtccccagc gtttccgtca gatggcgaac gatgtaatga
    22621 atgtcacggc ttttattctg ccagcgcagg cgcagcggca ggtcgctgat aacgcgcatc
    22681 agcagagatt cccagccgtg atcggggtga aagtctttcg ccagcgggcg gggaatggta
    22741 cgaaagcggc gctctggctg ggagctaaag ataaaaagcc gctcgggagt aagcgagcgg
    22801 tggtcaaata accgacagta cacggagtta aaaaagctct ccgcaatctc gaagcgcggg
    22861 taatccggca acagccgggt gtaatgctct ttgacgcgca gtaaaaatgc cgcgtccgtg
    22921 ctttggccgt tggtaatgca gcgcagttgc tccacgacca gacccacgtg atgatcgtaa
    22981 agatggatac ggtttttcat cgcctgctgg acagcgtgcc agtcggcttg ttcgaaacgc
    23041 tgctgcgccc cggaagtcac ttcgaggaat cgaccatact gagcgtcgaa accttgcaaa
    23101 atggtttgtg caatcagtaa ttccaggcca cgcggcatcg cctctcctta attcgcccct
    23161 cgccttacgg aaaggggcgt tggttgttag aactgcgatt cttcggtgga gccggtcagc
    23221 gcggtgactg aagacgtgcc gccctgaata atggtcgtca ctttatcgaa ataacctgta
    23281 cccacttcct gctggtgaga aacgaaggtg taaccatctt tcgcggcggc gaattccggt
    23341 tgctgcactt tctcaacgta gtgcttcata ccttcgccct gggcataggc gtttgccagg
    23401 tcaaacatgt tgaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag
    23461 cccatatccg acagctgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc
    23521 cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggatg
    23581 gcttgtgcaa agcgacgcgc cagttccagg tctggcgtgg aggtttcaca ccataccagg
    23641 tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta
    23701 cggaagaagc cttcgctagt acgctcgccg gtaataaatt cgctgtcata tgggtcgcaa
    23761 tcggaggtaa tcagatccgc cgcgtcggca tcggtacgag caaccagcag ggttggaacg
    23821 cccgtcacat cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttgga
    23881 accaaaactt tgccgcccat gtgaccgcat ttcttcactg atgccagctg atcttcgaag
    23941 tgaactgccg ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca
    24001 ccaccaaaac cggcttccgc atcagcaacg atcggcagga agtaatcgac atagcgcgga
    24061 tcgcccggct caatgcccgc ggaccattgg atctgatccg cacggcggaa ggtattgttg
    24121 atccgctcca ccaccgctgg caccgaattt gccggataga gcgactgatc cggatacatg
    24181 ctggccgcca ggttggcgtc cgccgctacc tgccatcccg acagatagac cgcttcaata
    24241 cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag
    24301 ccttttttcg actcgccgtg cagcagacgc cacattttcg ccgcgcccag ttgcgccagc
    24361 gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcgct gtaagggcga
    24421 gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta
    24481 cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc
    24541 ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg
    24601 cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt
    24661 tcatctcttc gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca
    24721 acgttttttg atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg
    24781 catcttccat caggccataa atcggcacac agccgttgcc agagatccat gcttcgatgt
    24841 actgcacagc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag
    24901 gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact tcaagctgat
    24961 ttttacggga gccgagaatg tcgttgaata ccgccattgc cgtatccgca aggcctgggt
    25021 gggcaatcca tgtgccatcg tgaccgttat tagcttccag cgatttatcc gcttttactt
    25081 tgttgagcac ctggttattg cgctcttcat ctttgctcgg aataaatgcc gccataccgc
    25141 ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag taagcattca
    25201 ggaagggttt atccatcgtc actgcctgtc tgtctggcag gacgcgatcg ggatagtttt
    25261 tcaacgtttt gatataactg aagatgtaat cccaacgacc gcagttcaga ccaacaatat
    25321 ggtcacgcag cgcgtgaagg atttcgtcca tctggaacac ggcgggcagc gtttcaatca
    25381 gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga
    25441 cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac
    25501 cgctgccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca
    25561 ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc
    25621 gtacccgaca aatcaacacc gctggattgg gcttgagctg gtaaattttg cctgcttcat
    25681 tggtatagct gatggtgccg ttaactgcat cacgcaggtt aatttgcccg tcgatcactt
    25741 tgttccagtc cggggccagt gaatcttcga aatcggccat aaagactttc acattggcgt
    25801 tgagcgcgtt gatcaccatc ttgcgctcaa ccgggccggt aatctctacc cgacgatctt
    25861 gtaagtccgc gggaatacca cgaattttcc agtcagcatc acgaatggaa gctgtttccg
    25921 aaataaaatc aggcaacgtt ccgttatcga tatcctgctg ctgctgaatg cgcgctgcca
    25981 gaagtttatt gcgttgtggc gtaaaatgcg tcaccagttc agtcagaaat tctaccgctt
    26041 cggcagtaag aatttgcttc tcctgctcgc catacggcct tatgaaagcc agttcatcgg
    26101 ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttccccac
    26161 tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa
    26221 tgcaaaatca aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa
    26281 tatgaataaa atttattcac ctactgaggt gcgtttcgga aataaaaaag gcacccgaag
    26341 gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc
    26401 gttggattca tgtgccgtag atcgtatggc gtgatctggt agacgtaata gttgagccag
    26461 ttggtaaaca gcaaattacc gtgactacgc cagctcgctc gcggtgtatt ttgcggatca
    26521 ttgtgcggga aatagttata cggtacttcc gggtctagtc cggcttccac atcgcggaaa
    26581 tattcctgcg ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc
    26641 ttatctttgc tggcaaacag ataggcatcc ccttcttctg tctccgccag aatttccaga
    26701 tcggtataat cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg
    26761 aatgaatcat caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa
    26821 acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg
    26881 gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc
    26941 acctgtttga tctgcggcca gtaagcgaca tcattaaact ctaccaggcc cagcggcgcg
    27001 ccggtaacta tcagaccgtc aaagttttgt tcctgaatat cttcaaagtt acagtagaag
    27061 ttattcagat gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga
    27121 atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcgatc
    27181 ttcttcggca tcaggttaag gataagcacc tttagcggac gaatttcctg accagacgcg
    27241 cgagaagttg tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg
    27301 tccggcacac gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca
    27361 tccagatagc tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga
    27421 gaaagtttca aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg
    27481 ttattagtat tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc
    27541 gttctgtcga tgccactcac gattttttat cagcagagta tcgggccgag ctggaagagc
    27601 tggtgcgttc ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgag cgggatcggc
    27661 cggttgggtt tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg
    27721 tgcgcggctg cggcgtgggt cggatgctgg tggaacatgc gctctcgatg gcaccggaaa
    27781 tgacaaccaa tgttaatgag caaaataagc aggctgttgg gttctataag aagatgggtt
    27841 ttaaggttac gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc
    27901 tggcgtatgt gggggaataa gctgcccctg ccgtgcctgc ccaggctgga gaagacgcgc
    27961 cagcgtcgca tccggcattt tttttgcacc aacgcaaaaa ggccatcctg acggatggcc
    28021 tttcgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
    28081 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
    28141 accgacaaac aacagataaa acaaaaggcc cagtctttcg actgagcctt ttgttt
//
LOCUS       DAYPHN010000049        28074 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000049 DAYPHN010000000
VERSION     DAYPHN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.049"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(222..2795)
                     /gene="clpB"
                     /locus_tag="ACG0BW_002039"
     CDS             complement(222..2795)
                     /gene="clpB"
                     /locus_tag="ACG0BW_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFK7259005.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2925..3656)
                     /gene="yfiH"
                     /locus_tag="ACG0BW_002040"
     CDS             complement(2925..3656)
                     /gene="yfiH"
                     /locus_tag="ACG0BW_002040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFK7259006.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     VHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLVDIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3653..4633)
                     /gene="rluD"
                     /locus_tag="ACG0BW_002041"
     CDS             complement(3653..4633)
                     /gene="rluD"
                     /locus_tag="ACG0BW_002041"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFK7259007.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPIYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4768..5505
                     /gene="bamD"
                     /locus_tag="ACG0BW_002042"
     CDS             4768..5505
                     /gene="bamD"
                     /locus_tag="ACG0BW_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFK7259008.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5776..6117
                     /gene="raiA"
                     /locus_tag="ACG0BW_002043"
     CDS             5776..6117
                     /gene="raiA"
                     /locus_tag="ACG0BW_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFK7259009.1"
                     /translation="MTMNITSKQMEITPTIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6220..6345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6221..6268
                     /gene="pheL"
                     /locus_tag="ACG0BW_002044"
     CDS             6221..6268
                     /gene="pheL"
                     /locus_tag="ACG0BW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFK7259010.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6367..7527
                     /gene="pheA"
                     /locus_tag="ACG0BW_002045"
     CDS             6367..7527
                     /gene="pheA"
                     /locus_tag="ACG0BW_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFK7259011.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7570..8691)
                     /gene="tyrA"
                     /locus_tag="ACG0BW_002046"
     CDS             complement(7570..8691)
                     /gene="tyrA"
                     /locus_tag="ACG0BW_002046"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFK7259012.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(8702..9772)
                     /gene="aroF"
                     /locus_tag="ACG0BW_002047"
     CDS             complement(8702..9772)
                     /gene="aroF"
                     /locus_tag="ACG0BW_002047"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFK7259013.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHHDLNGQLTARVA"
     gene            9981..10345
                     /gene="yfiL"
                     /locus_tag="ACG0BW_002048"
                     /pseudo
     CDS             9981..10345
                     /gene="yfiL"
                     /locus_tag="ACG0BW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
     gene            10492..11010
                     /gene="yfiR"
                     /locus_tag="ACG0BW_002049"
     CDS             10492..11010
                     /gene="yfiR"
                     /locus_tag="ACG0BW_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFK7259014.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTDCIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11000..12226
                     /gene="dgcN"
                     /locus_tag="ACG0BW_002050"
     CDS             11000..12226
                     /gene="dgcN"
                     /locus_tag="ACG0BW_002050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFK7259015.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIVQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEVAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12242..12724
                     /gene="yfiB"
                     /locus_tag="ACG0BW_002051"
     CDS             12242..12724
                     /gene="yfiB"
                     /locus_tag="ACG0BW_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFK7259016.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIARNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12800..13147)
                     /gene="rplS"
                     /locus_tag="ACG0BW_002052"
     CDS             complement(12800..13147)
                     /gene="rplS"
                     /locus_tag="ACG0BW_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFK7259017.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13189..13956)
                     /gene="trmD"
                     /locus_tag="ACG0BW_002053"
     CDS             complement(13189..13956)
                     /gene="trmD"
                     /locus_tag="ACG0BW_002053"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFK7259018.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13987..14535)
                     /gene="rimM"
                     /locus_tag="ACG0BW_002054"
     CDS             complement(13987..14535)
                     /gene="rimM"
                     /locus_tag="ACG0BW_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFK7259019.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14554..14802)
                     /gene="rpsP"
                     /locus_tag="ACG0BW_002055"
     CDS             complement(14554..14802)
                     /gene="rpsP"
                     /locus_tag="ACG0BW_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFK7259020.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15051..16412)
                     /gene="ffh"
                     /locus_tag="ACG0BW_002056"
     CDS             complement(15051..16412)
                     /gene="ffh"
                     /locus_tag="ACG0BW_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFK7259021.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16579..17370
                     /gene="ypjD"
                     /locus_tag="ACG0BW_002057"
     CDS             16579..17370
                     /gene="ypjD"
                     /locus_tag="ACG0BW_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFK7259022.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17391..18677
                     /gene="yfjD"
                     /locus_tag="ACG0BW_002058"
     CDS             17391..18677
                     /gene="yfjD"
                     /locus_tag="ACG0BW_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFK7259023.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18732..19325)
                     /gene="grpE"
                     /locus_tag="ACG0BW_002059"
     CDS             complement(18732..19325)
                     /gene="grpE"
                     /locus_tag="ACG0BW_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFK7259024.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19448..20326
                     /gene="nadK"
                     /locus_tag="ACG0BW_002060"
     CDS             19448..20326
                     /gene="nadK"
                     /locus_tag="ACG0BW_002060"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFK7259025.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20412..22073
                     /gene="recN"
                     /locus_tag="ACG0BW_002061"
     CDS             20412..22073
                     /gene="recN"
                     /locus_tag="ACG0BW_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFK7259026.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22222..22563
                     /gene="bamE"
                     /locus_tag="ACG0BW_002062"
     CDS             22222..22563
                     /gene="bamE"
                     /locus_tag="ACG0BW_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFK7259027.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22625..22915)
                     /gene="yfjF"
                     /locus_tag="ACG0BW_002063"
     CDS             complement(22625..22915)
                     /gene="yfjF"
                     /locus_tag="ACG0BW_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFK7259028.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDTVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22905..23342)
                     /gene="ratA"
                     /locus_tag="ACG0BW_002064"
     CDS             complement(22905..23342)
                     /gene="ratA"
                     /locus_tag="ACG0BW_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFK7259029.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23513..23995
                     /gene="smpB"
                     /locus_tag="ACG0BW_002065"
     CDS             23513..23995
                     /gene="smpB"
                     /locus_tag="ACG0BW_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFK7259030.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24210..24572
                     /gene="ssrA"
                     /locus_tag="ACG0BW_002066"
     tmRNA           24210..24572
                     /gene="ssrA"
                     /locus_tag="ACG0BW_002066"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24739..25986
                     /locus_tag="ACG0BW_002067"
     CDS             24739..25986
                     /locus_tag="ACG0BW_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFK7259031.1"
                     /translation="MPKQLKPLTNVEIAGAKPRSTDYELRDGEGLYLLVKTSGRKAWH
                     FEYYHPVTKKRTKTSLGPYPVVTLAMARETRTKYRRLLWQGIDPRQHLAGIAEEKRLQ
                     NECTLEKVAEQWLKEKKRTSDLSEDHAKDVWRSLEMHVFPSLGSTPVTEIRPKMLKEH
                     LTPLEEQGILETLRRVISRLNEIFRFAISEELIEFNPADNLVARFKKPKKQNMPALPP
                     SELGRLMLALQNASIRKETRCLIEWELLTWVRPGEAVSARWCDIDMKKAEWRIPDTFM
                     KMNRSHTVPLSKQALRVLQIMEPVSRHRPWVFPSIRKPLEHMHQQTANAALIRMGFGG
                     ELVAHGMRSIARTAGAGHFPREVLESALAHQKEDEIEAAYNRSDYLEQRRPLMQWWGN
                     YVDAARRQALLGEEEPLRIVEGE"
     gene            25988..26632
                     /locus_tag="ACG0BW_002068"
     CDS             25988..26632
                     /locus_tag="ACG0BW_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259032.1"
                     /translation="MTSPIRQNLFERECDMPLLEARHLALLLLGLDASQPANALPEEH
                     QENYRILHDAISRTIKATGMVSAPANKRMFYADEMFALAWRLIDDELTPPEIKARSLK
                     AVMKLSRNSRGRKWLKTLGDDALPDLTASTQPSLRGMHKRDKARENTARLCWLLVQLL
                     VDETDGRYGTSDKPASDRILRKLKELAQERELPSDGLGRGTFYEVLKMGREKEV"
     gene            complement(26982..>28074)
                     /locus_tag="ACG0BW_002069"
     CDS             complement(26982..>28074)
                     /locus_tag="ACG0BW_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259033.1"
                     /translation="PVRAVIQSWGRGLLERSGEQTKFINEFQTTFNSSMWELYLNEMF
                     IRLGYSVDYTKDSPDFCVSTPLGYKFNVEAMVSDKPHKPPVSEIFSEAKFKHQSTLKL
                     LGKIKDKRDLFTGINGKKFPYSTMEHVIGKPFVLALAPFDSDLSLTQNNTIINRVLFG
                     IEEPTMRDLVNGRQRRVLSIKKNEDTEIPLGIFTNDSYKEISAIVFSTTGTFGKAIVQ
                     SRVDRYVRYTRFRALDVNVFIANEGMKNEGSHTRKLGPDHYVTTKRQFYNNEVVGADI
                     VMCRTRDYKETHFDGLHVYYNPYASVPLDKNVFFPPEITHNFYDIENDIPDQRHPDGA
                     LVSRQVFEISKVCRRHIINNWFPEQSKVL"
BASE COUNT         7167 a         7396 c         6631 g         6880 t
ORIGIN      
        1 cttttctctg ttgagattct caggagaacc ccgctgaccc ggcggcgtgt ttgccgttgt
       61 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagcg aaaaatataa
      121 aaactttatc gttcgctcat ttttcaggca aaacatctta aatatagtct tttccgtcta
      181 atttatagac aaaaacgagc cccgaagggc tcgttttatc attactggac ggcgacaatc
      241 cggtcttcat taacttccag gcgaataact ttacccggaa ccaattcacc agacagtatt
      301 tgctgtgcca gcgggttttc gatctgctgc tggatagcac gtttcagagg acgtgcacca
      361 tagaccggat cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt
      421 tcataaccac gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgac
      481 gcaatgtgct gttcacccag cggatggaag accaccactt catcgatacg gttaatgaat
      541 tccggacgga agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc
      601 agttcaccga agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc
      661 gtattacgga agtcgaccgt tctcccctgc ccgtcagtca gacgcccatc atccagtacc
      721 tgcaacagaa tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg
      781 gaatacggac gacgacgcac cgcttcggtc aggtagccac cttcttcata accgacatat
      841 cccggaggcg caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg
      901 atacggacca tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca
      961 gttttcccca cacccgttgg gccgaggaac aggaatgaac caatcgggcg attcgggtca
     1021 gcaagccctg cgcggctacg acgaatagcg ttagataccg catcaaccgc ttcgttctga
     1081 ccaataacgc gatggtgcag ctcttgctcc atacgcagca gtttttcgcg ctcgctttcc
     1141 atcatgcgag aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcatcg
     1201 gtcactttat tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt
     1261 tgcttttcca gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtcaccc
     1321 acacggcgag cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgt
     1381 gtaccagaaa gcgatgcttt ctctgctttc cactcttctt ctaactcgga gtactgacgt
     1441 tctttgtcgc tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac
     1501 tctttcatta acgcctgttg ttccagttta agctggatga tacgacgatc gagtcggtcg
     1561 agttcttctg gttttgagtc gatctgcata cgaatgctgg atgctgcttc atcgatcagg
     1621 tcgatggctt tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct
     1681 gcaacaattg ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg
     1741 ccacgcagaa tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa
     1801 cgacgttcca gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca
     1861 cctacgcagt gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt
     1921 gcgccatcgg ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg
     1981 ttgccttcct gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg
     2041 cgatatttcg ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc
     2101 aacccttccg gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt
     2161 ttaccgacgc cgggttcacc aatcagtact gggttatttt tagtacgacg ttgcagcacc
     2221 tgaatggtac ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg
     2281 gctcgttcgg taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct
     2341 tgatcgttca cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg
     2401 gtcgcccctg ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga
     2461 acgaacagtt ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga
     2521 ttaagaacgc gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc
     2581 ggtaaacgat ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg
     2641 gatgttaata aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga
     2701 agtggttcga tgaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca
     2761 agctggaatt tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt
     2821 tgctactgga gattaaatga ggtcatccct caattattca aggttattga ccagattaat
     2881 gtgaaaagaa aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg
     2941 aaacttgcca tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc
     3001 gtatatgtac aacggtcgcc gccgaaaatt tgttcaacac caacgctcgc cagacgctgc
     3061 cgggcaagct gataaatgtc caccagatac ttatcaccat gctgaatgaa agctgtactc
     3121 gcttcggcgt ctactgccat aaacgcctcg cgaacctccg cccccacttc aaacgcgcgt
     3181 ggaccaattg ccggccctaa ccaggcgaga atattttctg gattatcagc aaaacaggaa
     3241 actgtctctt ccaacacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact
     3301 tccgttcccg ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa
     3361 accgtgccgg gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca
     3421 gtaagcttaa gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa
     3481 ttgcccgcag caaaaagtcg cttgcgattc tcctcaacgt gaaccgggtt atcgccacaa
     3541 tgagcaccga ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagtagag
     3601 ctacaggccg caacaccttt tggcagcggc cactgcggga caatcagctt actcataacc
     3661 agtccacttc atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca
     3721 tatcttgtgg aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac
     3781 gcagcatggt tgcatgaagc gcctggcggt caaacttacg cagcgtggag ataaacgctt
     3841 ccgaagcacc ttttggagga cgcggacggc caccataaat cggatcgccc accagcggat
     3901 gagtgatatg ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc
     3961 gcagacgcgt gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gctggtttgc
     4021 ccatcggatg caccgccatg tgagtacgtt tggtcgggtg gcgactgatt ggctcatcca
     4081 ccgtaccgcc agcggtcata tgaccaattg cgacagcttc atactcacga gtaatttcac
     4141 gccgttgcaa agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca
     4201 ggccagtggt gtctttatcc agacgatgga cgatgcccgc acgcggtaca tcggcaatgg
     4261 gcggatagta atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat
     4321 gtaccaccag gtcgcgtggt ttattaatga cgatgatatc ttcatcttca tagacgatat
     4381 ccagcgggat atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcca
     4441 cctgctcgcc acccaatact ttttctttcg gcttatcaca aactttgccg ttaaccagca
     4501 cgcgctggtc gaggatccat tcttttatcc gcgaacgtga ataatccggg aacatttcgg
     4561 ccaaagcctg atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta
     4621 ctcgttgtgc catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt
     4681 taatatagtg tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta
     4741 ttacattttg aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc
     4801 acactaagcc tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataacccg
     4861 ccaaatgaaa tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca
     4921 ataacgcaac tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg
     4981 cagctggatc tcatctacgc ctactataaa aacgccgatt tgccgttagc acaggctgcc
     5041 atcgatcgtt ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg
     5101 cgtggcctga ccaatatggc gctcgatgac agtgcactgc aagggttctt tggcgttgac
     5161 cgtagcgatc gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg
     5221 cgcggctatc cgaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa
     5281 gatcgtctgg cgaaatatga atactccgtt gccgagtact atacagaacg tggtgcatgg
     5341 gttgccgtcg ttaaccgcgt agaaggcatg ttgcgcgact acccggatac ccaggctacg
     5401 cgtgatgcgc tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct
     5461 gaaaaagtag cgaaaatcat cgctgcaaac agcagcaata cataacaaaa acctgaaaca
     5521 caaaacggca gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat
     5581 ttagtcacta tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa
     5641 cacctcttcc tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga
     5701 gatttccatc acacattttg acatcaggaa cggtatgctg aattcaccaa gacgggaaga
     5761 caagaggtaa aatttatgac aatgaacatt accagcaaac aaatggaaat tactccgacc
     5821 atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt
     5881 aatccacata ttattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat
     5941 acacctaacg gcgttctggt tgccagtggt aaacatgaag atatgtacac cgcaattaac
     6001 gaattgatca acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt
     6061 cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc
     6121 tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa
     6181 cagtacgggt actgtactga agtcacttaa ggaaacaaac atgaaacaca caccgttttt
     6241 cttcgcattc ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg
     6301 cgaagacgaa caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc
     6361 aacactatga catcggaaaa tccattactg gcgctgcgag agaaaatcag cgcgctggat
     6421 gaaaaattat tagcattact cgcagagcgg cgcgaactgg ccgtcgaggt gggaaaagcc
     6481 aaactgctct cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt actggaaaga
     6541 ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag
     6601 ctcatcattg aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa
     6661 attaatccac actctgcccg cattgctttt ctcggcccca aaggctccta ttcacatctt
     6721 gccgctcgtc agtacgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa
     6781 tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt
     6841 gaaaatacca gctccggtgc cataaatgac gtttacgatc tgctgcaaca taccagtttg
     6901 tcgattgttg gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcactact
     6961 gatttatcca ccatcaatac ggtctacagc catccgcagc cattccagca atgcagcaaa
     7021 ttccttaatc gttatccgca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg
     7081 gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc
     7141 actttgtacg gtttgcaggt actggagcgt attgaagcga atcagcgaca aaacttcacc
     7201 cgatttgtgg tgttggcacg taaagccatt aacgtgtctg accaggttcc agcgaaaacg
     7261 acgttgttaa tggcgaccgg gcaacaagct ggtgcgctgg ttgaagcgtt gctggtactg
     7321 cgcaaccaca atctgattat gacccgtctg gagtcacgcc cgattcacgg taatccatgg
     7381 gaagagatgt tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca
     7441 ttgaaagagt taggggacat cacccgttcg atgaaggtat tgggctgtta cccaagtgag
     7501 aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcacccggc
     7561 actggattat tagtggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg
     7621 aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc
     7681 ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt
     7741 gattaacgcc agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg
     7801 agcaaacagt cgcccaacca tcgccagttc aaggcggtaa atcggcgaag agagcgccag
     7861 aagttgctca agctgaacat tttcttctgc caggtgcagc ccataagcaa aagtagcaaa
     7921 gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg
     7981 cagccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg
     8041 tccatcacac cagaccacaa cttgctttgc caggctaccg ctgtccggac cgaacatcgg
     8101 gtgtagcccc agcaccggac catcatgcgc cgccagcatg gcctgtaatg gcccattttt
     8161 cactgatgcc agatcgacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac
     8221 ttgctcagta acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatttc
     8281 agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg agagtgtcag
     8341 catcttctcg aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag
     8401 tgacggacaa agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac
     8461 ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg
     8521 acgcgaggcc aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct
     8581 tttcacctcg cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc
     8641 tttatcgact tcatcaattt gatcgcgtaa tgcggtcaac tcagcaacca taataaacct
     8701 cttaagccac gcgcgccgtc agctgcccgt tgagatcatg atggatctca cgcagtaagg
     8761 catcggtcat ttcccagcta atgcaggcat cggttacaga cacgccgtat ttcatttcgc
     8821 tacgcggctg ttcggaagac tgattacctt cgtggatatt actttcaatc atcagaccaa
     8881 taatggagcg attaccatct ttgatctgag caaccacgga ctctgccacc gcaggctgac
     8941 ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc
     9001 ccgcctgttc catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt
     9061 taccaccgcg caggatcaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct
     9121 gccctgcctg gttaatgcca acaaaacggt gcggctgggc ggcggcacgc atagcgttaa
     9181 ttgccgttgc aaggctgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg
     9241 aggccatttc acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa
     9301 acagatcgcc caggtattgc gggctatttg gatctaacgc ttccgtcgcc agtggcagtc
     9361 ccatattcac cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa
     9421 aagagccatc catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt
     9481 tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt
     9541 taaatcgacg agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata
     9601 ctaccaacag acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag
     9661 caatctgggc ttcttgttgc aggcttaatg gaaaagcggc cttcagttgt tccggagtca
     9721 ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga
     9781 tcctgtttat gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca
     9841 taaagtaaag ttttcaatcc atatttcgta catttttctt tacaggaaca atttactcgc
     9901 gcttttgatg cttattttgt acaaataaat ttacacctcc tttaaaaaca atccgctatg
     9961 ctttgaaaaa ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg
    10021 ttagcggatg tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt
    10081 atgatgtcgg tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta
    10141 gcgattcaca ggcggatcgc ggtctatacc ttaaaggata tgccgaagga caaaagaaaa
    10201 cttgccagac cgattttact tatgcccgag gactttctgg taaaagcttt cctgccagct
    10261 gcaataatgt tgaaaatgcc agccaactgc atgaagtatg gcgaaaaggg ctgatgaaag
    10321 tgccagcaca atacgattaa attaattacc ccccaaataa attaagcatg agattttact
    10381 cattatgttt tatttctcac attgatgacg actgcatgaa aaaatgatat tttaatgagc
    10441 attctgaaaa aatatcgatg catttcgagc gaagatggtg aggatccctg aatgcgtttt
    10501 tctcaccgac tgttcttact gctgattctc ctgctgacgg gagcaccaat actcgctcag
    10561 gaaccttctg acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc
    10621 cgctggcctg cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt
    10681 accgccctac aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact
    10741 caacaagagg cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca
    10801 ttccagatgg aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa
    10861 aataccgatt gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga
    10921 tttgccgtaa acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta
    10981 atgctcgcac ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt
    11041 taaaagagca ttacgcaaca tcagtatgac cagcatattt atcactatga tgctgatctg
    11101 gttgctgctt tcagtgacct cggtgctgac cctgaaacag tacgcgcaaa aaaacctggc
    11161 actgacagca gcaacaatga cttacagtct ggaagcagcg gtcgtttttg ccgatggccc
    11221 tgcagcaact gaaacactgg cggcgctggg gcagcaaggg caattttcaa ctgcagaagt
    11281 gcgtgataag cagcaaaata ttctggcatc ctggcattac acccgtaagg atccaggcga
    11341 tactttcagt aatttcataa gccactggct cttccctgcc cccatcgttc agccgattcg
    11401 tcacaatggt gaaaccattg gcgaagttcg cttaaccgct cgcgacagtt caatcagcca
    11461 tttcatctgg ttttcgctcg ctgtcctgac cgcctgcatt ctgctggcat caggcatcgc
    11521 aattaccctc acccgccatt tgcacaatgg tctggttgaa gcgttaaaaa acatcaccga
    11581 tgtcgtacat gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat
    11641 cgcagagttt caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg
    11701 gcagcttcgt ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt
    11761 aaccgggctg gctaaccgcg cagcgtttcg tagcggtatc aatacgttga tgaacaattc
    11821 cgatgcccga aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa
    11881 tgatacctgg ggtcatgcga cgggcgatcg tgtcttgatt gaggtagcaa aacggttagc
    11941 tgaatttggc gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgcgatggt
    12001 gctctatgat gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat
    12061 ctttaatctc ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg
    12121 ttacgcgatg accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa
    12181 tatgtatcag gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga
    12241 tatgataaag cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca
    12301 gtcccctcag ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac
    12361 tgaatccgcc ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga
    12421 ctacaaattg ctcccggaaa gccagcaaca gatccaaacc atggcagcca aattggcctc
    12481 gacagggcta acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta
    12541 caacgaaggc ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg
    12601 acaaattcca cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagcccg
    12661 taacaagacc gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc
    12721 ttaaaacacg agcaaacaga taaaaaaggg ccagccaatt ggccagccct tcttaacagg
    12781 atgtcgctta agcgaaacct tagttaagac gctctttgat acgagcagcc ttaccagtac
    12841 gctcacgcag gtagtacagt ttagctttac gaacagcacc acgacgtttg acagaaatgc
    12901 tgtcaactac cggagagtga gtctggaaga cacgctcaac gccttcgccg ttggaaattt
    12961 tacgaacagt gaatgcagag tgcagaccgc ggttacgaat agcgataacc acgccctcga
    13021 atgcctgcag acgttttttg gaaccttcaa caacccatac tttcacttcc acggtatcac
    13081 ccggacggaa ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc ttaataatgt
    13141 tgctcataat ttaatctctt atcctgggta aactgatatc tcgggggctt acgccatccc
    13201 atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca accttgcttg
    13261 ctcttcagtc agagccaggt tttccagaag ttcaggtctt ctaagccagg tacggcccag
    13321 cgactgtttc aaacgccagc gacgtatctc ggcatggttg cccgacagta acactggcgg
    13381 aacttccatc ccttctaaca cctcaggccg cgtatagtgc gggcaatcca gcaatccttc
    13441 agcaaaggaa tcttccgttg ctgaggcttc atgtcccagt actcccggaa taaaccggga
    13501 aacggagtca atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt aatcgccgat
    13561 tgaccattct tcgtcaattt cggtttggat cacgcgctca tctatacctt cgtagcgacc
    13621 gcacaccaga atcaattttt gattcgttgc cagttcgctg acgcccgctt gatcaagctt
    13681 gcgtccctgt ggtgaaagat aaatcacctt tgcgccttca cccgccgcgg cttttgctgc
    13741 atgaatggcg tcccgcaagg gttgcaccat cattaacatc cccggtccgc cgccgtaagg
    13801 acgatcgtcc acggtacggt gccggtcatg cgtgaagtcg cgaggactcc agctctggat
    13861 gctcagcagg ccatttttaa ctgcccggcc agttaccccg taatcggtaa ttgcgcggaa
    13921 catttcagga aacaggctaa ttatgccaat ccacatagcg ccgtctttta ccgtttatcc
    13981 ggtggtttaa aaaccaggat cccaatctac ttcgattgaa cgagtagtga gatcgacttt
    14041 cttgataacc tgcccatcga ggaacggtac gagacgttcc ttgataccaa acgcatcttt
    14101 caggtttgcc ttaatgacga gaacgtcatt agatccggtt tccatcatat cgacgacttt
    14161 accgagatcg tagccttcag tggttactac ctggcagccc atcaggtctt tccagtagta
    14221 gtcaccctct tcaagctgag gcagctgcga tgaatccacg acaatttcac aattcgtcag
    14281 caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt cctgattgtg
    14341 gtgcttccag ctttccagct ggacttgctg ccactgaccc gccttctgga taaaccaggg
    14401 ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa aacactctga gccacccacg
    14461 aataccgtaa gacgaaccca tttttcccaa aacgatggga tcaacaggtg cttgcgcggt
    14521 gagttgtttg ctcatcatga ccaccgtgac agattaagct gctttgttta cttctttgat
    14581 cagcgcagca acgcgatcag aaatagttgc gccctggcca acccagtgag cgatgcgatc
    14641 cagatccagg cgagtgcctt cttctttttc gctagcgatt gggttgaaga aaccaacgcg
    14701 ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg acaacaacct ggtagaacgg
    14761 acgcttttta gcgccgtgac gtgctaaacg aatagttacc ataacatcct cttgtgtgaa
    14821 taaaacaacc ggaccccatc gaggaacgga gtccggtgtc atattaaaag cccgaaaatt
    14881 ttactcattt ttgcggaaat tgcaatcaac agttgctaac tctgctgtaa aaggccgtcg
    14941 gcggtgcagc cagtttggca ccggagtgcg cgcagtcacc ggagcgtaca cgcagtacgt
    15001 gaggatgacg agcacacccc ggtgccaaaa tggcaaacaa gccaggccga ttagcgacca
    15061 ggaaatcccg gcggcatcat acccttcatg cttctcatca tcttcgccat tccgcccttc
    15121 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
    15181 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gtgaaccttt gatgatttct
    15241 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
    15301 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
    15361 atcaagctgg ccatgccgcc catatttttc atctgccgca gctgctcaag gaagtcgttg
    15421 agatcgaagc cgtcgccttt tttcagcttg ctggctaatt tctctgcctg cgcgcggtca
    15481 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
    15541 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgagaaac
    15601 ttgatcggtt tgccagtgat gtgacgaata gagagtgccg caccgccgcg ggcatcgccg
    15661 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattaaatgc ttttgccgta
    15721 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cggattaatc
    15781 gacgcatgga cttgtttgat ctcatccatc atcgcttcgt caacgtgcag acgaccagcg
    15841 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaacg
    15901 atatctaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    15961 agcgtctcaa gctgtttgat tgccgccggg cgataaacgt cggcggaaac caccagcact
    16021 ttcttcttgt gcttctcgcg caggaactta ccgagtttac caacgctggt tgttttaccg
    16081 gcaccttgca ggcccgccat cagcacgacc gctggcggtt gcgcagccag gttcagggtc
    16141 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    16201 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    16261 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    16321 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    16381 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    16441 gccgcaatcg cgacacatca tcagtatatt gccgcagtat aacatgaagg cgtctttgtt
    16501 gttatgcaac ggttggagca gcgttcacct aacgctatac tgcttctctt tcttattgct
    16561 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    16621 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    16681 attatttctg cggtcatcgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    16741 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    16801 atgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    16861 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    16921 atcactcatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    16981 tatgccacgc tgattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    17041 ctgaagaaca agaagctggc gtttaaccag gaaatgccgc cattaatgag tatcgagcgt
    17101 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    17161 ctgttctaca tgcacaactt atttagcatg gaaaatatcg acaaggccgt gctctctatc
    17221 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt atcatgaagg atggcgtgga
    17281 cgccgcgtcg tctggtttaa cgttgcgggt gcagtcatcc tgacactggc ctacttcggc
    17341 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    17401 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    17461 ccgggtccga aaccggaatg atgaccctca accgctatcg tctgcgacat atggcgaaac
    17521 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgatta
    17581 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    17641 ttgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    17701 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    17761 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    17821 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    17881 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcacgaa tcgcgctcac
    17941 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aagatgaccg
    18001 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    18061 aatcgattct gcgccaactc tcccactcac ctcacgggcg catcgtgctc taccgtgatt
    18121 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    18181 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtcccgg
    18241 aaggtacgcc cctcagcacg cagttggtga agtttcagcg caacaaaaag aaagtcggcc
    18301 tggtcgtcaa cgagtatgga gacattcaag ggctggtgac ggttgaagat attctggaag
    18361 agatagttgg tgatttcacc acgtcgatgt cgccaacact tgccgaagaa gtcactccgc
    18421 aaaacgacgg ttcggtgatt attgatggca ccgccaacgt gcgggaaatc aacaaagcct
    18481 ttaactggca tctaccggaa gatgatgcac gaacggttaa tggcgtcatt ctggaagcgc
    18541 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat attgatattc
    18601 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    18661 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gatttattac
    18721 gaaagcagaa attaagcttt tgctttcgct acagtaacca tcgccgcacg aatcgtacga
    18781 ccgttcagcg tataaccttt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    18841 gattccacca ttgcgatggc ctgatgcaca ttcgggtcca gcgggacgtt agtttcggcg
    18901 atcacttcaa cgccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    18961 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    19021 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    19081 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacacgcaaa
    19141 atgccgtcac gttcacgggt ctgagcttca gccagctgag cttcgagatt cgcaattttt
    19201 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    19261 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    19321 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    19381 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    19441 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    19501 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    19561 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    19621 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    19681 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    19741 gggtttcctg actgaccttg accccgataa cgcccagcaa cagttagccg atgtgctgga
    19801 aggtcactac ataagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    19861 ccaaaagcgc atcagcaccg cgataaatga agtggtactt catccaggca aagtggcgca
    19921 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gttctgatgg
    19981 cctgattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    20041 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    20101 gcgaccgctg gtgataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    20161 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    20221 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    20281 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    20341 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    20401 ggaaaacaac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    20461 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggcgcg ggtaaatcta
    20521 ttgcaataga tgccctcggc ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    20581 ccggcgctgc tcgtgccgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    20641 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtaa
    20701 tcagcagcga tggtcgctca cgaggtttta tcaacggtac agcggttcct ctgtcacaac
    20761 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat cagttactca
    20821 ccaaacccga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctactcc
    20881 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    20941 aacagttaag tcaggaacgc gctgcccgtg cggaactgct gcaataccaa ttaaaagaac
    21001 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    21061 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    21121 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    21181 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    21241 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tttggatctc gatcccaacc
    21301 ggctgtttga acttgaacaa cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    21361 tcagccctga ggcattgcca cagtattacc agtcgctact ggaagaacag cagcaactgg
    21421 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    21481 cactggaaac ggcgcgcgcg ttacaccagc aacgccagca ctatgcagaa gaacttgcac
    21541 agctgatcac cgacagtatg catgcactct ctatgccgca tgggcagttt acgattgatg
    21601 ttaaatttga cgagcatcac ctgggcgctg acggcgctga tcgtattgaa tttcgggtaa
    21661 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    21721 cccgcatcgc actggcaatt caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    21781 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    21841 tgcgtcaact cggcgaatca actcaagtga tgtgtgttac ccacctgcca caagtcgcgg
    21901 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacgc
    21961 atatgcaatc cctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    22021 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcggcg taaacttttt
    22081 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    22141 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    22201 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    22261 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    22321 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    22381 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    22441 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    22501 acctttaaca gtagcggagt gttgaccaac attgataaca aacccgcgct gagtggtaac
    22561 taatcataaa gttgccctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    22621 tctgttattt attcgctgat ttttctgctc gttgcctgcg cagctctttc ggatcggcaa
    22681 tgagaggacg ataaatctcc acccgatcgc catcatgcac ggtatcgctg agttttgccg
    22741 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    22801 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc acgcgctgca
    22861 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct ggcactgtaa
    22921 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    22981 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    23041 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    23101 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    23161 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    23221 tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc
    23281 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    23341 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    23401 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    23461 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    23521 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    23581 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    23641 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaagc
    23701 gttcctgttt ggcgcaaaca tcacaccaat ggcagtggcc tccacgcatg tagtgtgcga
    23761 tcctacccgt acccgcaaat tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    23821 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    23881 caaagtgaaa atcggcgtcg ccaaaggtaa gaaacagcac gataaacgtt cagacatcaa
    23941 agaacgcgaa tggcaggtgg ataaagcgcg tatcatgaaa aacgcccacc gttaaacctg
    24001 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    24061 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    24121 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    24181 taaatctggt atacttacca ttacacattg gggctgattc tggattcgac gggatttgcg
    24241 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    24301 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    24361 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    24421 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    24481 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggacg taggaatttc
    24541 ggacgcgggt tcaactcccg ccagctccac caaacattca ttcatgatgc atcatgaacc
    24601 ttaaaaaagc ctgtaacttc aacgagttgc aggctttttt gttgcatgtg tggtcatgat
    24661 ctctgtatga atcttgacct tttggcacca tgtttagcac cacggtatcg tggggctaaa
    24721 aaagcgtggt gctaaaacat gcctaagcaa ctgaaaccct taacaaatgt ggagatcgct
    24781 ggcgcgaagc cccgtagcac ggattatgag ctacgggacg gtgaaggcct gtacttgctg
    24841 gttaagactt cagggaggaa ggcctggcat ttcgagtatt atcatcccgt caccaaaaag
    24901 cgcaccaaaa ccagccttgg cccttacccg gtcgttacac tggccatggc tcgcgaaact
    24961 cgcacgaaat acagacgact gctctggcag ggcatcgatc cccgtcaaca cttagcagga
    25021 atagccgaag aaaaacgcct ccagaatgaa tgcacgctgg aaaaagttgc ggaacagtgg
    25081 ctcaaagaga agaaacggac cagcgacctt agcgaagatc acgccaaaga tgtctggcgc
    25141 tcgctggaga tgcacgtctt cccttctctg ggcagtacgc ccgtcaccga gatccgcccg
    25201 aagatgctca aggaacacct caccccgctg gaagaacagg gcatcctcga aacgctgcgc
    25261 cgggttatct cacggctcaa tgaaatcttc cgcttcgcca tctccgaaga gcttatcgag
    25321 tttaacccag cagacaacct ggtcgcccgc ttcaagaaac cgaaaaagca gaacatgccc
    25381 gcccttcccc ccagcgaact gggcagacta atgttggcac tacaaaacgc ctctatccgc
    25441 aaggaaacac gctgccttat cgaatgggag ctactgacct gggttcgccc cggcgaagcc
    25501 gttagcgccc gctggtgcga catcgatatg aaaaaagcgg aatggcgtat ccctgacacc
    25561 tttatgaaga tgaaccgttc acacacggtg ccgctcagca aacaggcgct gcgtgttctt
    25621 cagatcatgg agcccgtcag ccgccatcgt ccgtgggtct tccccagcat ccgcaaaccg
    25681 ctggagcaca tgcaccagca gaccgcgaac gccgcgctta tccgcatggg gttcggcggt
    25741 gagctggtcg cccacggcat gcgcagcatc gccagaacgg cgggcgcagg tcattttccc
    25801 cgtgaagttc tggaatctgc actggcacac cagaaagaag acgagatcga agcggcctac
    25861 aatcgcagcg actatctgga acaaaggcga ccgctcatgc agtggtgggg aaactacgtt
    25921 gatgccgcca gacggcaggc attgctcggt gaagaagaac cgctgcggat cgtggaagga
    25981 gaataatgtg accagtccca ttcgtcagaa tctgtttgaa cgggaatgcg atatgccgct
    26041 gctggaagcg cggcatctcg cccttctgct tctcggcctt gatgcttccc aacctgcaaa
    26101 cgccctgccg gaagaacatc aggaaaatta ccgtatcctg cacgacgcca tcagtcgcac
    26161 catcaaagca acgggcatgg tcagcgcccc ggcgaacaaa cgcatgttct atgccgatga
    26221 aatgttcgcg ctggcctggc ggcttattga tgacgaactc acgccgccag aaattaaggc
    26281 ccggagcctg aaggcagtga tgaaactgtc acgcaacagc cgtggccgca agtggctgaa
    26341 gacgctcggc gacgatgcgc tgccggatct cactgcttcc acacaacctt ccctgcgtgg
    26401 gatgcataag cgcgacaaag cacgggagaa tactgccagg ctctgctggt tactggttca
    26461 gcttctggtt gatgagactg acggacggta tggcacgtct gataaaccgg cttcggatcg
    26521 gatacttcgg aagctgaagg agctggcgca ggagcgggaa ttaccgtcag acgggcttgg
    26581 gcgagggacg ttttatgagg ttttgaagat gggaagggag aaggaagttt aggtaataac
    26641 actgcagatc gcacgtgaac acagtgccac tttccctatg gaatgtgaaa ttaatcggta
    26701 atacccctcg aaagaatgtg aacagacact ctcttgctta actgttgaaa ctcttcgaat
    26761 taaagacaag gttcgccatt taattatctg acagttcgcc ccagcacaac gttaaagtga
    26821 tttttgatca atcacctact ctgctctggc agagccagag ccgttttact gactaaagca
    26881 caagcttcca gcgaatgcac ctttccacac accataagtc ttgtgtgata ccagcactga
    26941 cggtgtatgt tacatatcct taaaaagttc tccagaccga cttagagaac cttagactgt
    27001 tctgggaacc agttattaat tatatgtctt cgacagactt tagatatttc aaatacctgt
    27061 cgtgacacta atgcaccatc aggatggcgc tggtcaggta tgtcattttc tatgtcataa
    27121 aaattatgcg ttatttctgg gggaaagaaa acgtttttgt ctaaaggcac gcttgcatag
    27181 ggattataat acacatgcaa accatcaaaa tgagtttcct tgtagtccct tgttcggcac
    27241 attacaatat ccgcgccaac aacctcattg ttgtagaatt gtcgcttcgt agtaacataa
    27301 tggtcaggac caagttttct cgtatgagaa ccttcgttct tcataccttc attggcaata
    27361 aaaacattca catcaagagc tcggaatctc gtatatcgga catatcgatc gacccgactc
    27421 tgaacgatag ccttaccaaa agtacctgtc gttgaaaaca cgatagcact gatttctttg
    27481 tatgagtcat ttgtaaaaat acctaaaggt atttcagtgt cttcattttt tttaatcgat
    27541 aaaacccttc tttgcctacc atttactaaa tctctcatcg taggttcttc aatgccaaac
    27601 aatacccggt taatgattgt attattctgg gttaaagata gatctgaatc aaatggggcc
    27661 aatgctaaca caaaaggctt accgattaca tgttccattg ttgagtatgg gaacttctta
    27721 ccattaattc cggtaaagag atcgcgctta tctttaattt tcccaagaag ttttagcgta
    27781 gattgatgct taaacttagc ttcactgaaa atttcactga ctggcggttt atggggcttg
    27841 tcagagacca tagcctctac gttaaattta taacctaatg gcgtgctaac acaaaaatca
    27901 ggactgtctt ttgtatagtc aaccgaatac cccaaacgaa taaacatctc atttagatat
    27961 aattcccaca tagaagaatt aaaggtagtc tgaaattcgt taataaattt agtttgttca
    28021 ccactgcgtt ccagtaatcc gcgtccccat gattggatca ccgccctaac agga
//
LOCUS       DAYPHN010000050        27943 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000050 DAYPHN010000000
VERSION     DAYPHN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.050"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(8..814)
                     /gene="trpA"
                     /locus_tag="ACG0BW_002070"
     CDS             complement(8..814)
                     /gene="trpA"
                     /locus_tag="ACG0BW_002070"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFK7259034.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFSKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLVALKAFVQPMKAATRS"
     gene            complement(814..2007)
                     /gene="trpB"
                     /locus_tag="ACG0BW_002071"
     CDS             complement(814..2007)
                     /gene="trpB"
                     /locus_tag="ACG0BW_002071"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFK7259035.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMHLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2019..3380)
                     /gene="trpCF"
                     /locus_tag="ACG0BW_002072"
     CDS             complement(2019..3380)
                     /gene="trpCF"
                     /locus_tag="ACG0BW_002072"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFK7259036.1"
                     /translation="MMQTVLAKIVADKAIWVETRKEQQPLASFQNEVQPSTRHFYDAL
                     QGARTSFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQQYIDTLRGALPANIAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3381..4976)
                     /gene="trpD"
                     /locus_tag="ACG0BW_002073"
     CDS             complement(3381..4976)
                     /gene="trpD"
                     /locus_tag="ACG0BW_002073"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFK7259037.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVHGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMSVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(4976..6538)
                     /gene="trpE"
                     /locus_tag="ACG0BW_002074"
     CDS             complement(4976..6538)
                     /gene="trpE"
                     /locus_tag="ACG0BW_002074"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFK7259038.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMHCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6560..6653)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6630..6674)
                     /gene="trpL"
                     /locus_tag="ACG0BW_002075"
     CDS             complement(6630..6674)
                     /gene="trpL"
                     /locus_tag="ACG0BW_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFK7259039.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6812..7693
                     /gene="rnm"
                     /locus_tag="ACG0BW_002076"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6812..7693
                     /gene="rnm"
                     /locus_tag="ACG0BW_002076"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFK7259040.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7690..8310
                     /gene="yciO"
                     /locus_tag="ACG0BW_002077"
     CDS             7690..8310
                     /gene="yciO"
                     /locus_tag="ACG0BW_002077"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFK7259041.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8338..10233
                     /gene="yciQ"
                     /locus_tag="ACG0BW_002078"
     CDS             8338..10233
                     /gene="yciQ"
                     /locus_tag="ACG0BW_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFK7259042.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGGLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGVPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLKDQYDKNQQILIRV
                     NEGATRNNAVLLPAEKLLLEALFRKGDKVVLTGRRNRVLRSAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNCVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGI
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAERITPSLTAAVSAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            10444..11319
                     /gene="rluB"
                     /locus_tag="ACG0BW_002079"
     CDS             10444..11319
                     /gene="rluB"
                     /locus_tag="ACG0BW_002079"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFK7259043.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(11359..11949)
                     /gene="cobO"
                     /locus_tag="ACG0BW_002080"
     CDS             complement(11359..11949)
                     /gene="cobO"
                     /locus_tag="ACG0BW_002080"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFK7259044.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERKLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVKALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11946..12704)
                     /gene="yciK"
                     /locus_tag="ACG0BW_002081"
     CDS             complement(11946..12704)
                     /gene="yciK"
                     /locus_tag="ACG0BW_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFK7259045.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLHQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRANAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12924..13973
                     /gene="sohB"
                     /locus_tag="ACG0BW_002082"
     CDS             12924..13973
                     /gene="sohB"
                     /locus_tag="ACG0BW_002082"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFK7259046.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITSVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLNIDQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14009..14260)
                     /gene="yciN"
                     /locus_tag="ACG0BW_002083"
     CDS             complement(14009..14260)
                     /gene="yciN"
                     /locus_tag="ACG0BW_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFK7259047.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14640..17237
                     /gene="topA"
                     /locus_tag="ACG0BW_002084"
     CDS             14640..17237
                     /gene="topA"
                     /locus_tag="ACG0BW_002084"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFK7259048.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGDALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17447..18421
                     /gene="cysB"
                     /locus_tag="ACG0BW_002085"
     CDS             17447..18421
                     /gene="cysB"
                     /locus_tag="ACG0BW_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFK7259049.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            18740..18880
                     /gene="ymiA"
                     /locus_tag="ACG0BW_002086"
     CDS             18740..18880
                     /gene="ymiA"
                     /locus_tag="ACG0BW_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFK7259050.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            18883..19050
                     /gene="yciX"
                     /locus_tag="ACG0BW_002087"
     CDS             18883..19050
                     /gene="yciX"
                     /locus_tag="ACG0BW_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFK7259051.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTTQQQAFI
                     ELFGEDDQPKQ"
     gene            19164..19259
                     /gene="ymiC"
                     /locus_tag="ACG0BW_002088"
     CDS             19164..19259
                     /gene="ymiC"
                     /locus_tag="ACG0BW_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFK7259052.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            19423..22098
                     /gene="acnA"
                     /locus_tag="ACG0BW_002089"
     CDS             19423..22098
                     /gene="acnA"
                     /locus_tag="ACG0BW_002089"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFK7259053.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALVGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEIYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSEVISIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIVDLQNLQPGATVPVTFTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(22162..22752)
                     /gene="ribA"
                     /locus_tag="ACG0BW_002090"
     CDS             complement(22162..22752)
                     /gene="ribA"
                     /locus_tag="ACG0BW_002090"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFK7259054.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            22922..23686
                     /gene="pgpB"
                     /locus_tag="ACG0BW_002091"
     CDS             22922..23686
                     /gene="pgpB"
                     /locus_tag="ACG0BW_002091"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFK7259055.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEENNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRVCGPLTPPAEENREIAQREQES"
     gene            23835..24143
                     /gene="lapA"
                     /locus_tag="ACG0BW_002092"
     CDS             23835..24143
                     /gene="lapA"
                     /locus_tag="ACG0BW_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFK7259056.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            24150..25319
                     /gene="lapB"
                     /locus_tag="ACG0BW_002093"
     CDS             24150..25319
                     /gene="lapB"
                     /locus_tag="ACG0BW_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFK7259057.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            25511..26248
                     /gene="pyrF"
                     /locus_tag="ACG0BW_002094"
     CDS             25511..26248
                     /gene="pyrF"
                     /locus_tag="ACG0BW_002094"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFK7259058.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            26248..26574
                     /gene="yciH"
                     /locus_tag="ACG0BW_002095"
     CDS             26248..26574
                     /gene="yciH"
                     /locus_tag="ACG0BW_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFK7259059.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(26700..26918)
                     /gene="osmB"
                     /locus_tag="ACG0BW_002096"
     CDS             complement(26700..26918)
                     /gene="osmB"
                     /locus_tag="ACG0BW_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFK7259060.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(27187..27936)
                     /gene="yciT"
                     /locus_tag="ACG0BW_002097"
     CDS             complement(27187..27936)
                     /gene="yciT"
                     /locus_tag="ACG0BW_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFK7259061.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6832 a         6807 c         7372 g         6932 t
ORIGIN      
        1 tgtgggatta actgcgcgta gccgctttca tcggttgtac aaaagctttc agtgccacca
       61 gcattttctc tggctcatta atatgttgct cgatgatttt aacaatggct gaacctgaaa
      121 tcgcgcctgc agctcctgca tcaatcgctg cttttacctg atccggggcg gaaataccaa
      181 agccctgcaa tggaggcgca gcattgtact ctttcagctt cgtgaccaga tgattgaggg
      241 gtaacgcggc gcggttttct gcgccggtca cgcccgcacg tgacaataaa taggtgtatc
      301 cacgaccgta ggaggctatc tggcgcagca aatcatcgtc ggcattcggc gggcaaataa
      361 agataggtgc gacattatga cgcaacgcgg cctggcggaa gggagcggac tcttccactg
      421 gcacatcggc aaccagcacc gaatcgacgc cgactttctc gcactgggca taaaactcat
      481 caatgccttt gctaaacacc aggttggcat acatcaaaag gccgatggga atggtcgggt
      541 gcttctggcg aatcagtgcc agcatctcaa agcactgtgc cggggtcaca cctgccgcaa
      601 aggcacgcag tgtggcgttt tgaatcgtcg ggccatccgc cagtgggtcg gagaagggga
      661 tacctaactc cagcgcgtca gcaccggctt caattagcgt gtcgataatt ttcagcgact
      721 gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa tgcgccttct ttgcgctcct
      781 tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg ctttcaaaat
      841 atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca ccagtagctg
      901 ctctttttcc gggttttcgc gcatcatttt cagcgcatgg gccagggcgt gggaggactc
      961 cagcgccggg atgatccctt cgtgcaggca cagcgttttg aaggcttcca gtgcttcatc
     1021 atcggtaatt gacacgtaat cagcgcgtcc agtgctgtta agatacgcgt gttgtgggcc
     1081 gacagacggg aaatccagtc cggcggagat ggagtaagat tcttcaatct gtccgtcttc
     1141 ggtttgcatc atcggcgctt tcataccgaa atagatgccc acgcgaccgt gttttaacgg
     1201 tgcgccgtgc tcgccagttt cgataccgtg accgcctggt tcgacaccaa tcaggccgac
     1261 gtcggtttcg ttgataaaat cagcaaacat gccgatggca tttgaaccgc cgccaacaca
     1321 agcgataacc gcatccggca ggcgaccttc tctttccaga atctgtgctt tggtttcttc
     1381 gccaatcatc cgctggaact cacggacaat ggtcggataa ggatgtgggc cagctgcggt
     1441 gcccagcata tagtgcgcgg tttcataact gccggaccag tcgcgcagcg cctcattaca
     1501 ggcatctttc agcgtcgcgg aaccgctatg caccgggatc acttccgcgc ccattaaatg
     1561 catacgaaaa acgttaggcg actggcgttc aacgtctttg gcacccatat aaatacggca
     1621 tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac cggcaccggt
     1681 ttcggcgatg atttcggttt tacccatccg cttcgccagt aaagcctgac cgagcacctg
     1741 gttagtttta tgcgcgccgc cgtgcagcaa atcttcgcgc ttgagataca gcgtggtgtt
     1801 agtgccagcc gtgatgttct ggcatttggt gagtgcggtt ggacgcccgg catagttttt
     1861 cagcaggtcg ttgaactggg cctgaaattc aggatctttt tgcgcgctga caaaagcttc
     1921 ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc caaactcacc
     1981 aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg cagcgtctgg
     2041 aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt gcgactctac agcagaatta
     2101 aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc gcctaagccc
     2161 cccgccagca gaacgttgcc aagcgattga ccgtttaaca gtgaccagtc gaaacgttgc
     2221 ccgcttccac cctggccgtt gtctaaaaca tatttatcaa cgtgctgaag ctcgcgggcg
     2281 ggcagggttt caccgacgct taatgctttc cagatggcaa tgtttgcggg cagcgcgcca
     2341 cgcaatgtgt cgatatattg ttgatcttca ttaccatgca gttgcactgc cgcaagtgat
     2401 aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac gccaacatac
     2461 tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca acgcggtgat
     2521 gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagctgc tttagcatct
     2581 tgcccacgcg tcaggccaca tactttattc tcacccagca acacccggcg cacggcggcg
     2641 ttcaaatcgt catgggccat caacgccgaa ccaatcagaa aaccgttagc gaagtggctt
     2701 aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt cacgttgtgc
     2761 cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc acgcagatcg
     2821 cggttgttga tgccaacgac ctttgccccc aatgcaatgg cacgctccag ttcctcttca
     2881 ttactgactt cggtcagcac acccatctcc agactgtggg cgacggcagc aagctggcga
     2941 tattgttcgt catccagtac tgaaagcatt aataagcagg catcggcctg gtaatagcgc
     3001 gccagataga tctggtaagg atcgataatg aagtctttac ataaaatcgg ctgcggggcg
     3061 atttggctga cgatggggag gaaatcaaag ctcccctgaa aatatttctc atcagtcagc
     3121 accgaaattg ccgaagcgta atgtttataa atggcggcaa tgcgtgccgg atcgaaatca
     3181 tcacggatca cgccttttga cggcgacgct tttttgcact ccagaataaa cgacgtgcgt
     3241 gcaccctgaa gtgcatcata aaaatgtcgc gtgctcggct gaacctcatt ctgaaaactg
     3301 gccagcggtt gctgctcttt gcgggtttct acccaaatcg ccttgtctgc gacgattttc
     3361 gctaaaacgg tttgcatcat ttaccctcgt gccgccagtg cggtgactct gtcgtaagcg
     3421 gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc ttcatggcca
     3481 tgcaggcgca ttaacatggc gacattcgcc gcgacggctg cttcatgggc ggcgtcgcct
     3541 ttaccttgta acaagcgtgt taaaatgtca cggttttctt ccggtgttcc gcctgccagt
     3601 tgctcctggt ggtagggtgt caggccaaaa tcttctgcag ttagctgata gctcttaatt
     3661 tcaccgtcat gcagttcggc aacgattgtc ggcgcgtgta atgaaacttc atccatcccg
     3721 ccgctgtgca ctaccgcagc gcgttgatac cccagcacgc gcaaggtttc ggcaatcggc
     3781 agcaccagtt ccggactata aacaccaatt agcgccagcg gcggatgcgc cgggttaatc
     3841 aatggcccca gcacattgaa cagggtgcgg gttttcagtt gctggcgaac cgacatcgcg
     3901 tggcggaatc cggtgtgata tttcggcgca aagaggaaac agacgcctaa ctcatccagc
     3961 gcctggcgcg atttatcggc gttcatatca agattaatac cgaacgccgc cagcagatcc
     4021 gacgagccgg atttactgga gacgctacgg ttgccgtgtt tcgccacttt cagcccacag
     4081 gccgcggcga caaaagcact ggcggtagaa atattgatac tgttgctgcc gtcaccaccg
     4141 gtaccgacga tatcggcaaa cagataatcc gggcgcggga acggcgcggc gttttccagt
     4201 agcgcggttg ctgctccggc gatttcgttt gggtgctcgc cgcgaatttt catgcttacc
     4261 agagccgccg cgagctgttc tggcttcagc tcgccatgca ccactgctga aaacagctgg
     4321 tggctttctt gctggctaag cgtctgcgcc tgatacagtt tttccagaat cggttgcagc
     4381 gtgttagtcg gttccagttt ctgctgcgcc caggccaatg tttgttccag caggcgagcg
     4441 ccctgggtag taagaatgga ttccggatgg aactggaatc cacaaacgcg atctgcatcg
     4501 tgacgcactg ccatcaccat gccattaaaa tgggcgttga tggttaaacc ggccggaatg
     4561 ttactgccaa ccagcgagtg ataacgcgcc actggcagcg ggtttgttaa tccggcaaac
     4621 atcgcctgac cgtcgtgttc aatgctggag gctttaccgt gaagaatttc gcccgcctga
     4681 ccgacatacc ccccataagc ttcgacaatc gcctgatgac cgaggcaaat gccaataatt
     4741 ggcagcttgc cgcgcaggcg ggtgaggagt tctggcatac aaccggcttc gcttggcaca
     4801 ccggggccag gagaaagcat cagcaccgga ttgctcattg tcgccaggcg ttcaattaag
     4861 gtctgcgccg gaatatggtt gcggtaaatc accacgttat gaccattgct gcgcaactga
     4921 tctgccaggt tgtaagtaaa agagtcgata ttatcgagca gcagaatgtc agccatcaga
     4981 acgtctcctg tgcatgatgc gcggtggcaa tagcgcgcag tacagcgcgg gctttattac
     5041 gggtttcgtc ggcttccgac tgcggaacag aatcaagtac cacgccagca ccggcttgca
     5101 cggtggcgat accgttttcc accagtgccg agcggatcac aatgcaggtg tcgagatcgc
     5161 catgcgcggt gaaataacct accgcgccgc cgtagctgcc gcggcgacga ccttccgcct
     5221 cggcaattaa ctgcatagcg cgtactttcg gtgcaccgct taacgtcccc atattcatac
     5281 aggcgcggta agcgtgcagg gcgtcgagat cgtggcgcag ctcaccgacc acgcgggaga
     5341 ccaggtgcat cacgtaagag taacggtcaa ctttggtgag atcggcgacg tagcggctgc
     5401 cgggggtgca aatgcgtgcc agatcattac gggcgagatc caccagcatc agatgttcag
     5461 aaagctcttt atgatcggta cgcatttcca gttcgatgcg gctgtcgagg tctctgtcca
     5521 gcgaaccatc ggcacgacga ccgcgtggac gtgttccggc aatcgggtag atctcaatct
     5581 ggcggctggt ggcgtcatac ttgagcgagc tctccggcga cgcgccaaac agggtgaaat
     5641 cattatcctg cataaaaaac atatacgggc tgggattact ctttttcagc acgtaatagg
     5701 ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc tggaaaattt
     5761 ctccggcgcg aatcgctttt tgcaacaaac gcactacgcc accgaactct tcatcgctct
     5821 ggttacattc acaatgcata tgcggcacgg aaaccaccgg cagcggtggc gcggtttcgg
     5881 tcagctgctg gcgaagatcg ttcaggcgag cagtgagacg ttgtttttct tcttcattcg
     5941 gagcaaaaag actggcctga atgcgggtgc tttttttctg atggtcaatc accatcagcg
     6001 tttcagcgag ataaaaacag aaatcagggc agttattttc cgctgacagt tgcggtaaat
     6061 cttcaaaccc cgccacaagg tcataagaga acaggccgcc gaagaacatt gcttctcgtt
     6121 cttccttcgg tacattcaac agattctgca ataagcggaa agcgtcaaaa accgaaaggg
     6181 agcataagcg ggcgtcttca tccagcagtg gactgacagg ggggaagcgc agcacacggc
     6241 agtttggtga ttgttcattt tccacacccg caggcagggc gttatccagt agtgccaaca
     6301 gggcttcgcc attgccggaa agtgcctgaa ttgtgacagt gtcacctaat gctgtaatgc
     6361 gcagcgcact gtctaccagc agcaggcttt ttaaatcatc tttgctgtcg atatctgcgg
     6421 attccagcag cagcgttgct ggacgattcc cacacaactg gtgaaaaagc gcagtcgggt
     6481 tgtcgcgata agcgccttcg caggttagca gttcaagagt cggtttttgt gtttgcattg
     6541 ttattctcta attttgttca aaaaaaagcc cgctcatcag gcgggctggg tatctgattg
     6601 ctttacgcat ggtgaataca ctgcccgttt caggaagtgc gccaccaacc tttcagtacg
     6661 aaaattgctt tcattgtcga tacccttttt acgtgaactt gcgtactagt taactagttc
     6721 gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg
     6781 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattacg cagtgattta
     6841 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
     6901 ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca ccacagctgc
     6961 aatctcacca gcaagagagg aaatttcacg ttcgggactg gcgctgaatc ttattcccgg
     7021 cgtggaaatc tccacggtct gggaaaatca tgaaattcat attgtcgggc tgaatattga
     7081 tattactcat ccgctaatgt gtgagttcct cgcgcagcag acagaacggc gcaatcagcg
     7141 ggcgcagctg attgccgaaa gacttgaaaa agcgcagata ccaggtgcgc tggaaggcgc
     7201 gcaacgactg gcgcagggcg gtgcggtgac gcgcggtcat tttgctcgtt tccttgtgga
     7261 gtgcggcaaa gccagttcaa tggcggatgt ctttaaaaaa tatctggcgc gcgggaaaac
     7321 cggatacgtt ccgccacagt ggtgtacaat agaacaagct attgatgtca ttcatcattc
     7381 tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta aatggctgaa
     7441 aagattggtt gcgcattttg ctgaacacca cggcgacgcg atggaagtcg cgcagtgcca
     7501 gcaatcgccc aatgaacgca cccagctggc gacacttgcg cgtcagcatc atttatgggc
     7561 atcacaagga tctgattttc atcagccgtg cccgtggatt gaactgggtc gtaaactctg
     7621 gctgcctgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga acaccacaga
     7681 gagggaatta tgagccagtt tttttatatt catcctgata atccgcagca acgtctgatc
     7741 aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tttatccaac cgattccggc
     7801 tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg agcgtatttg tcgaattcgc
     7861 cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga gctctcgacc
     7921 tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa acaacacgcc gggcaactac
     7981 accttcatcc tgaaagggac gaaggaggtg ccacgccgtc tgttgcagga aaaacgcaaa
     8041 accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga agcactgggc
     8101 gaaccgatgc tttccacttc gctaatgctg ccaggcagcg aatttaccga atccgatccg
     8161 gaagaaatta aagaccgtct ggaaaaacag gtggacttga ttattcacgg cggctatctc
     8221 gggcagaaac caacaacggt tattgatctt actgatgata cgccggtcgt ggtgcgtgaa
     8281 ggcgtaggtg atgtgaagcc tttcttataa caaattgcgt tccacggatg gaagactatg
     8341 gcagggatat atcgctgcat tttgctgttg atagttgggc tttttttctc ttcgttgagt
     8401 tatccgaaaa acacggagat ccctttttat gaagaaggga tctcgctctt tgatgttgaa
     8461 gccactctgc aaccggatgg ggggctcgac atcaaagaaa atattcattt tcaggcgcga
     8521 aatcagcaga ttaagcacgg attttatcgt gatttaccac gactctggat gcagcctgat
     8581 ggggacgctg cactgctgaa ctatcatatt gttggcgtca cccgtgatgg tgttcctgaa
     8641 ccctggcatc ttgactggca tatcggttta atgagtatcg tcgtgggcga taaacaacgt
     8701 ttcttgcctc aaggcgacta tcattatcaa attcattatc aggttaaaaa tgctttcctg
     8761 cgtgagggag attcagatct gttaatctgg aacgtgactg gtaaccactg gccgtttgaa
     8821 atctataaga cccgattttc actcaagttc cctgatatcg cgggtaatcc atttagcgaa
     8881 atcgatctct ttactggaga agagggcgac acatatcgaa atggccgcat ccttgaggac
     8941 ggaagaattg aatcccgcga tccgttttat cgtgaagatt tcacggtact ctaccgctgg
     9001 cctcacgctt tacttagcaa tgccccggct ccacaaacga cgaatatttt cagccatctt
     9061 cttttaccct ccacgtcatc gttgttaatt tggtttccgt gtctattcct ggtttgtgga
     9121 tggttatatc tctggaagcg caggccgcaa tttacgccgg tagatgtgat tgaaaccgat
     9181 gtcattccgc cagattacac gcccggcatg ttacgtctcg atgcgaagct ggtttacgac
     9241 gataaaggtt tttgtgccga tatcgtaaat ctgattgtga aaggcaaaat tcatctgaaa
     9301 gatcagtatg acaagaacca gcaaatcctg attcgtgtta atgaaggcgc gaccagaaat
     9361 aatgcggtat tactgcccgc agagaagtta ttactggaag cgttatttcg taaaggcgat
     9421 aaggtcgttc ttacggggag acgcaacaga gtcttacgca gtgcattttt acggatgcag
     9481 aaattttatc tgccgcgtaa aaagtcttcg ttttaccgac ctgatgcgtt tttgcaatgg
     9541 gggggaatgg cgatattagc ggttattctc tacggtaacc tgagtcccgt aggctgggca
     9601 ggaatgagtc tggtgggcga tatgtttatt atgatctgct ggcttcttcc ttttttattt
     9661 tgttcccttg agcttttgtt tgcccgcgat gatgacaagc cttgcgttaa ttgtgtaatc
     9721 atcactttgt ttttaccgct gatttgttca ggcgtggcct tttattctct ctatatcaat
     9781 gtcggagatg tattctttta ctggtatatg ccagcgggtt attttagcgc tgttttcctg
     9841 accggttatc tcactggcat agggtatatt tttctgccaa agtttaccca aactgggcag
     9901 caacgttatg cccacggtga agctatcgtt aactatcttg cgcgtaaaga ggcggcaaca
     9961 cacagtgggc ggcggcggaa aggggaaaca cggaaactgg attacgcatt gttaggttgg
    10021 gcggtctcgg caaaccttgg cagagaatgg gcagaacgta tcaccccatc actcacagcg
    10081 gctgttagcg ccccggaaat tgcccgtagt ggcgttttat tctcattgca gacgcacctg
    10141 agttgcgggg cctataccag tttgttgggg cgaagctatt ccggtggtgg tgctggcggc
    10201 ggtgcgggtg gcggaggcgg tggtggttgg taaccaaatc gacgttaatc gaaacaagct
    10261 ttaaccataa agatagtgta gtatgttgcg cctcaaagcc aggtcgataa acgtcgagtc
    10321 gtttacttaa ggccttaaga gttcaaacag actttactat cagtgagtca gatgtaagtt
    10381 ctgactcact gttgcacaaa agcatatcag acgcctggga aggcgacacc aaaaggaagc
    10441 tctatgagcg aaaagctaca gaaagtgctg gcgcgtgccg gccacggctc tcgccgtgaa
    10501 atcgaatcta ttattgaagc cggtcgtgtg agtgttgatg gcaaaattgc taaactcggc
    10561 gatcgcgttg aaattacccc tggattgaaa atccgtatcg atggtcacct gatttcggta
    10621 cgtgagtccg ctgaacaaat ttgtcgcgtt ctggcctatt acaaaccgga aggtgagttg
    10681 tgtacccgta acgatccgga aggacgtcca acggtgtttg accgtctgcc aaaactgcgt
    10741 ggcgcacgct ggattgccgt aggtcgtctg gacgttaaca cctgtggtct gttgctcttc
    10801 accaccgacg gtgaactggc aaaccgttta atgcatccaa gccgtgaagt tgaacgtgaa
    10861 tatgccgtgc gtgtatttgg tcaggttgac gacgcgaaac tgcgtgattt gagtcgtggc
    10921 gtgcagctgg aagatggtcc ggcagccttt aaaaccatca agttcagcgg cggcgaaggg
    10981 atcaaccagt ggtacaacgt tactctgacc gaaggccgta accgtgaggt tcgtcgtctt
    11041 tgggaagcgg ttggtgtgca ggtaagtcgc ctgatccgtg tgcgttacgg tgatattcca
    11101 ctgccgaaag gtctgccacg cggcggctgg acagagctgg atctcgccca gactaactat
    11161 ctgcgcgaac tggtggagct accgccagaa accagctcta aagtcgctgt agaaaaagac
    11221 cgtcgtcgta tgaaggcgaa tcagattcgt cgtgcggtta agcgtcacag tcaggtgagc
    11281 ggcggtcgtc gctctggtgg acgtaataat aacggttaat caaagtatgc cggacgtcat
    11341 atccggcatt tttacagatt agtaatcaat ccctatctgc gcttttacac cggcatcaaa
    11401 cgcatgtttc acggggcgta attcacttac cgtatccgcc aattcaagaa tatcccgatg
    11461 acaaccacga cccgtgataa tcaccgtttg ttgatgtgga cgttcattta acgccttcac
    11521 cacttcttcc agtggcaaat aatcatacgc caccatataa gtcagttcat cgagcaaaac
    11581 catatccagt gaaggatctg ccagcattcg tttggcatgt tgccagactt cacggcaggc
    11641 cgcggtatca gattcgcgat tttgtgtatc ccaggtaaag cccgttgcca tcacctgaaa
    11701 ttcaacgccg tgtggttcca gcagttttcg ttcgccgtta ggccaggtgc ctttaataaa
    11761 ctgcacgacg cccacttttt ttccgtgacc aactgcgcgt gttgccgtac caaatgccgc
    11821 ggtggttttg ccttttccat tgccggtaaa gacgatgata ataccgcgtt catcctgggc
    11881 ctgggccaca cgagcatcta ctttttcttt cactcgctgc tgacgctgtt ggtagcgttc
    11941 atcactcatt gggaaattcc tggtttacgg cccggttggg cgtcaaaggt catgccggtt
    12001 ttacggcggc tgtcatcgcc catcagccag aggtagagcg gcatgatatc agcgggtgtt
    12061 ttaagtttct gtggatcttc ggtcgggaag gcgttggcac gcattgcggt gcgcgtaccg
    12121 cctgggttaa tgcagttgac gcgcaggcgc tgctgatatt catcggccag tacctgcatc
    12181 atcccttcgg tggcaaattt cgacgctgca tatgcgcccc agtttgcacg cccctgacgt
    12241 ccaacgcttg atgaagtaaa gaccagtgaa cccgcgtccg atttgagtaa taaaggaagc
    12301 agtgcttggg tgagcataaa ggtggcatta acgttgacct gcatgacgtc ctgccagacc
    12361 tgcggatttt gttcgctcat tgggcaaaca tcgccgagca atccggcatt atgcaaaaca
    12421 ccatccagac gtggataatt aacggcaatg cgctgtgcca gttgttggca attttcggac
    12481 gtgcaggtca gcaaatcgag aataaaccac tgtggctgac gcccagtttc ttcgtttatg
    12541 tggctggcta cctgatgtaa tttttcttca ttacggccca acagaatcac agtcgcacca
    12601 tagcgtgcat acgtcatcgc ggcttcacga ccaatcccat cgctggctcc cgtcaccagg
    12661 ataatgcgat cattgagtaa atcttgtttt ggctggtaat gcatggctac tcctcaacga
    12721 cgttgtctgt cgcgctgcca gtgtacttta agactttggg gctttatgcc tgaaacagaa
    12781 gggcatttca atcagcctgg ggcgataaca ccgcaaaatg gatactttgt catactttcg
    12841 ctgcaataac atctctgcga gacggcttaa catgcctgtt gtaaactgtg agccaaagcg
    12901 ttgtttaacc aaggtgggga ctcgtggaat tgttgtctga atatggtttg tttttggcga
    12961 aaatcgttac cgttgtgcta gcgattgcgg cgattgccgc cattattgtc aatgttgctc
    13021 aacgtaataa acgccagcgt ggcgagttac gggtcaacaa tctcagcgaa cagtataagg
    13081 agatgaaaga agaactggcc gcggcgctga tggactcaca tcagcaaaaa cagtggcaca
    13141 aagcgcagaa gaaaaagcac aagcaagaag cgaaagcagc aaaagcgaaa gccaaactgg
    13201 gcgaggtggc aactgacagt aaacctcgcg tctgggtgct ggattttaaa ggcagcatgg
    13261 acgcccatga agtgaactcg ctacgtgaag agataacgtc ggtactcgca gcatttaaac
    13321 cgcaggatca ggttgtgcta cgtctggaaa gccctggtgg catggtgcat ggttacgggc
    13381 tggcggcttc gcagctgcag cgtctgcgtg ataaaaacat tcctttaact gttacggtag
    13441 acaaagtcgc tgccagcggc ggttacatga tggcctgtgt ggcggacaaa attgtttccg
    13501 caccgtttgc tattgtgggt tccattggcg tggtggcaca aatgcccaac tttaaccgct
    13561 tcctgaaaag caaagatatt gatatcgaac tgcacaccgc cgggcagtat aagcgcacgc
    13621 tgaccttgct gggtgaaaac accgaagaag ggcgggagaa attccgcgaa gagctgaacg
    13681 aaacgcatca gttatttaaa gattttgtga agcgtatgcg tccgtctctg aatattgatc
    13741 aggtggcaac gggtgaacac tggtacggac aacaggcggt agagaaaggc ttggttgatg
    13801 aaatcaacac cagtgatgaa gttattctta gcctgatgga aggccgtgaa gtggtcaatg
    13861 tacgctatat gcagcgtaaa cgactcattg accgattcac cggcagcgcg gcagagagcg
    13921 ccgatcgatt gttgctacgc tggtggcagc ggggtcaaaa gccattgatg taaaagacaa
    13981 acgcgagggt aagacctcgc gttttgcttt aatcaaccag gtgatatttt tctgaaagca
    14041 catgggccag gtgtttgaac atattaaaca ccgcggtgct tttggctgtt ggcaatcctt
    14101 gttcatctaa aaagtagtcg ccggtaaata ccagcacgcc attacgctgc gtgacgccgg
    14161 tggcttcaat ccctgcgagc gtatcctcat gctcacgaat gattttgttg gcctctttca
    14221 acagcgtctc gcggtcgatg ggttgtgtct ctttgttcat tatttactcc ttaaacaagg
    14281 acattagtct acgccaggca tggcttgcag acaaatatac cacgctggtg gcaagagcgc
    14341 cttactggca actttggatt ttgcatgcta ataaagttgc gtatcggatt ttatcaggta
    14401 cagtgtgacg ctttcgtcaa tctggcaata gatttgcttg acattcgacc aaaattccgt
    14461 cgtgctatag cgcctgtagg ccaagacctg ttaactcagt cacctgaatt ttcgtgaaca
    14521 gagtcacgac aaggggttga tatccgcaga gagcgagtcc atatcggtaa ctcgttgcca
    14581 gtggaaggtt tatcaacgtg cgacgcattc ctggaagaat caaattaggt aaggtgaata
    14641 tgggtaaagc tcttgtcatc gttgagtccc cggcaaaagc caaaacgatc aacaagtatc
    14701 tgggtagtga ctacgtggtg aaatccagcg tcggtcacat ccgcgatttg ccgaccagtg
    14761 gctcagctgc caaaaagagt gccgactcta cctccaccaa gacggctaaa aagcctaaaa
    14821 aggatgaacg tggcgctctc gtcaaccgta tgggggttga cccgtggcac aattgggagg
    14881 cgcactatga agtgttgcct ggtaaagaga aggtcgtctc tgaactgaaa caactggctg
    14941 aaaaagccga ccacatctat ctcgcaaccg accttgaccg cgaaggggaa gccattgcat
    15001 ggcacctgcg ggaagtgatt ggtggtgatg atgcgcgcta tagccgagtg gtgtttaacg
    15061 aaattactaa aaacgcgatc cgccaggcat ttaacaaacc aggtgagctg aatattgatc
    15121 gtgttaatgc ccagcaggcg cgtcgcttta tggaccgcgt ggtgggatat atggtttcgc
    15181 cgctgctgtg gaaaaagatc gctcgtggtc tgtctgcagg tcgtgtgcag tcggtggcgg
    15241 tccgcctggt ggtcgagcgt gagcgtgaaa ttaaagcgtt cgtaccggaa gagttctggg
    15301 aagtcgatgc cagcacgacc acgccatctg gtgatgcgtt ggcgttgcag gtgactcatc
    15361 agaacgacaa accgttccgc ccggtcaaca aagaacaaac tcaggctgcg gtaagtctgc
    15421 tggaaaaagc gcgctacagc gtgctggaac gtgaagacaa accgacaacc agtaaacctg
    15481 gcgctccttt tattacctct acgctgcaac aagctgccag cacccgtctt ggttttggcg
    15541 tgaaaaaaac catgatgatg gcgcagcgtt tgtatgaagc tggctatatc acatacatgc
    15601 gtaccgactc caccaacctg agtcaggacg cggtaaatat ggttcgcggt tatatcagcg
    15661 ataattttgg taagaaatat ctgccggaaa gtccgaatca gtacgccagc aaagaaaact
    15721 cacaggaagc gcacgaagcg attcgtcctt ctgacgtcaa tgtgatggcg gaatcgctga
    15781 aggatatgga agcagatgcg caaaaactgt accagttaat ctggcgtcag ttcgttgcct
    15841 gccagatgac cccagcgaaa tatgactcca cgacgctgac cgttggtgcg ggcgatttcc
    15901 gcctgaaagc acgcggtcgt attttgcgct ttgatggctg gacaaaagtg atgcctgcac
    15961 tgcgtaaagg cgatgaagat cgtatcttac cggcagtcga taaaggcgat gctctgacgc
    16021 tcgttgaact gacaccagcc cagcacttta ccaagccgcc agcgcgtttc agtgaagcat
    16081 cgttggttaa agaactggaa aaacgcggta tcggtcgtcc gtctacctat gcgtcgatca
    16141 tttcgactat tcaggatcgt ggctatgtgc gagtagaaaa tcgtcgtttc tatgcggaaa
    16201 aaatgggcga aatcgtcacc gatcgcctgg aagagaattt ccgtgagtta atgaactacg
    16261 acttcaccgc acagatggaa aacagcctcg accaggtggc aaatcacgaa gcagagtgga
    16321 aagctgtact ggatcacttc ttctcggatt tcacccagca gttagataaa gctgaaaaag
    16381 atccggaaga gggggggatg cgcccgaacc agatggttct gaccagcatc gactgcccga
    16441 cctgtggtcg caaaatgggt attcgcacag cgagcaccgg ggtattcctt ggctgttccg
    16501 gctacgctct gccaccgaaa gagcgttgca aaaccaccat caacctggtg ccggaaaacg
    16561 aagtgctgaa cgtgctggaa ggtgaagacg ctgaaaccaa cgcgctgcgc gcaaaacgtc
    16621 gttgcccgaa atgcggcacg gcgatggaca gctatctcat cgatccgaaa cgtaagttgc
    16681 atgtctgtgg taataaccca acctgcgacg gttacgagat cgaagagggc gaattccgca
    16741 ttaaaggtta tgacggcccg atcgttgagt gtgaaaaatg tggatctgaa atgcacctga
    16801 aaatggggcg tttcggtaaa tacatggcct gcactaacga agagtgtaaa aacacgcgta
    16861 agattttacg taacggcgaa gtggcaccac cgaaagaaga tccggtgcca ttaccggagc
    16921 tgccgtgcga aaaatcagat gcttatttcg tgctgcgtga cggtgctgcc ggtgtgttcc
    16981 tggcggccaa taccttcccg aaatcgcgtg aaacacgtgc gccactggtg gaagagcttt
    17041 atcgcttccg cgaccgtctg ccggaaaaac tgcgttatct ggccgatgcg ccacagcagg
    17101 atccggaagg taataagacc atggttcgct ttagccgtaa aaccaaacag caatatgtct
    17161 cttcggaaaa agacggaaag gcgactggct ggtcagcatt ttatgttgat ggcaaatggg
    17221 ttgaagggaa aaaataacct ttaattctgt caggttttta taaacaaagg gtcgcgaaag
    17281 cggccctttt ttattgcata ttattttttc ttcacaccta tacactaagg ctataaatga
    17341 tatagtggtt atagttagca ccttttttat tattaaatcg tattagtcac ccgccaggtg
    17401 tgacgaaaaa acgatgttct gatggcgtct aagtggatgg tttaacatga aattacaaca
    17461 acttcgctat attgttgagg tggtcaatca taacctgaat gtctcatcaa cggcggaagg
    17521 actttacaca tcacaacccg ggatcagtaa acaagtcaga atgctggaag acgagctagg
    17581 cattcaaatt ttttcccgaa gcggcaagca cctgacgcag gtaacgccag caggacaaga
    17641 aataattcgt atcgctcgtg aagtcctgtc gaaagtcgat gccataaaat cggtcgccgg
    17701 agagcacacc tggccggata aaggctcgct gtatatcgcc accacgcata cccaggcacg
    17761 ctatgcgtta ccaaacgtca tcaaaggctt tattgagcgt tatcctcgcg tttctttgca
    17821 tatgcaccag ggctcgccga cacaaattgc tgatgccgtc tctaaaggca atgccgattt
    17881 cgcgattgcc acagaagcgc tgcatctgta tgaagattta gtgatgttac cgtgctacca
    17941 ctggaatcgg gctattgtag tcactccgga tcacccgctg gcaggcaaaa aagccattac
    18001 cattgaagaa ctggcgcaat atccgttggt gacatatacc ttcggcttta ccggacgttc
    18061 agaactggat actgccttta accgcgcagg gttaacgccg cgtatcgtct tcacggcaac
    18121 ggatgctgac gtcattaaaa cttacgtccg gttagggctg ggagtagggg tcattgccag
    18181 catggcggtg gatccggtcg ccgatcccga tcttgtgcgt gttgatgctc acgatatctt
    18241 cagccacagt acaaccaaaa ttggttttcg ccgtagtact tttttgcgca gttatatgta
    18301 tgatttcatt cagcgttttg caccgcattt aacgcgtgat gtcgttgatg cggctgtcgc
    18361 attgcgctcc aatgaagaaa ttgaggtcat gtttaaagat ataaaactgc cggaaaaata
    18421 atttcttgcg ttatttccgg caccttttat gtagagaagg tgccggaata tattctcttt
    18481 tgttgcttat ttgtatccgc tgcacaatat ttttatccct catatttata gggtaaatta
    18541 cttgtaaagt agtgtctaat tacccgcctc gcaaactcca gttttcaaat atctatttca
    18601 ggtcaaaaga ttgaattatt cactggagac gattcgtaaa ttcgctggat ttttgactaa
    18661 agtttcttta ggatttgtct catcgtatga ttaattacac caatcgttca acgtcttgct
    18721 gataagtgat ggcgattata tgaggttagc aatgccttct ggaaatcagg aaccccgccg
    18781 ggaccctgaa ttaaaacgca aagcctggct ggcggtattt cttggttctg cacttttctg
    18841 ggtggttgtc gcactgctga tttggaaagt gtggggataa ctatggtcgg tcaggagcaa
    18901 ctggagtcgt caccattatg tcagcatagt gacaatgaac cagaaacgaa acgggaatgt
    18961 tccgtcgtta ttccagacga ctggcaacta acaacgcagc agcaagcctt tatagaactg
    19021 tttggtgaag atgatcagcc gaaacaataa ttatcatcat tcttattacc catttttaat
    19081 caattaaagg gcttttaata caccgcagca ataacaggtt gagttatctc aacacaaagt
    19141 aataaccgtt aagggtgtag cctatgatca acacaaatat gaaatattgg tcctggatgg
    19201 gcgcattttc tctgtcgatg ctcttctggg ccgaactcct ctggatcatt actcactgat
    19261 ccctgacccc gctgcggcgg ggttttcatt tgctttgcca caaggtttct cctcttttat
    19321 caatttgggt tgttatcaaa tcgttacgcg atgtttgtgt tatctttaat attcaccctg
    19381 aagagaatca gggcttcgca accctgtcat taaggaggag ctatgtcgtc aaccctacga
    19441 gaagccagta aggacacgtt gcaggccaaa gataaaactt accactacta cagcctgccg
    19501 cttgctgcca aatcactggg cgatatcacc cgtctgccta agtcactcaa agttttgctc
    19561 gaaaacctgc tgcgctggca ggatggtaac tcggttaccg aagaggatat ccacgcgctg
    19621 gtgggatggc tgaaaaatgc tcatgctgac cgtgaaattg cctaccgccc ggcaagggtg
    19681 ttgatgcagg actttaccgg cgtacctgcc gttgttgatc tggcggcaat gcgcgaagcg
    19741 gttaaacgcc tcggcggcga tactgcaaag gttaacccgc tctcaccggt cgacctggtc
    19801 attgaccact cggtgaccgt cgatcgtttt ggtgatgatg aggcatttga agaaaacgtg
    19861 cgcctggaaa tggagcgcaa ccacgaacgt tatgtgttcc tgaaatgggg aaagcaggcg
    19921 ttcagtcgtt ttagcgtcgt gccgccaggc acaggcattt gccatcaggt taacctcgaa
    19981 tatctcggca aagcagtgtg gagcgaattg caggacggtg aatggattgc ttatccggat
    20041 acactcgttg gtactgactc gcacaccacc atgatcaacg gccttggcgt gctggggtgg
    20101 ggcgttggtg ggatcgaagc cgaagccgca atgttaggcc agccggtttc catgcttatc
    20161 ccggacgtag tgggctttaa actcaccgga aaattacgtg aaggtattac cgccacagac
    20221 ctggttctca ccgttaccca aatgctgcgc aaacatggcg tggtggggaa attcgtcgaa
    20281 ttttatggtg atgggctgga ttcactaccg ctggcggatc gcgccaccat tgccaatatg
    20341 tcgccagaat atggtgccac ctgtggcttc ttcccaatcg atgctgtcac cctcgattac
    20401 atgcgtttaa gcgggcgcag tgaagatcag gttgagttgg tcgaaatata tgccaaagcg
    20461 cagggcatgt ggcgtaaccc gggcgatgaa ccgattttta ccagtacgtt agaactggat
    20521 atgaatgacg ttgaagcgag cctggcaggg ccaaaacgcc cacaggatcg cgttgcactg
    20581 cccgatgtac caaaagcatt tgccgccagt aacgaactgg aagtgaatgc cacgcataaa
    20641 gatcgccagc cggtcgatta tgttatgaac ggacatcagt atcagttacc tgatggcgcg
    20701 gtggtcattg ctgcgataac gtcgtgcacc aacacctcta acccaagtgt gctgatggcc
    20761 gcaggtttgc tggcgaaaaa agctgtaact ctgggcctca agcggcaacc gtgggtcaaa
    20821 gcgtcgctgg caccaggctc gaaagtcgtt tctgattatc tggcaaaagc gaaactgaca
    20881 ccgtatctcg atgaactggg atttaacctt gtgggatacg gttgtaccac ctgtattggt
    20941 aactctggtc cattgcccga tcctatcgaa acggcaatca aaaaaggcga tttaaccgtc
    21001 ggtgcggtgc tgtccggcaa ccgtaacttt gaaggccgta tccatccgct ggttaaaact
    21061 aactggctgg cctcgccgcc gctggtggtt gcctatgcgc tggcgggaaa tatgaatatt
    21121 aacctcgctt ctgagcctat tggtcacgat cgtaaaggcg acccggtata tctgaaagat
    21181 atctggccct cggcacaaga aattgcccgt gcggtagaac aagtctccac agaaatgttc
    21241 cgcaaagagt acgcagaagt ttttgaaggc acagcagagt ggaaggaaat taacgtcgca
    21301 cgatccgata cctacggttg gcaggaagat tcaacctata ttcgcttatc gcctttcttt
    21361 gatgaaatgc aggcaacacc agcacctgtg gaagatattc acggtgcgcg gatcctcgca
    21421 atgctggggg attcagtcac cactgaccat atctctccgg cgggcagtat taagcccgac
    21481 agcccggcgg gtcgatatct acaaggtcgg ggtgttgagc gcaaagactt taactcctac
    21541 ggttcgcggc gtggtaacca tgaagtgatg atgcgcggca ccttcgccaa tattcgcatc
    21601 cgtaatgaaa tggtgcctgg cgttgaaggg gggatgacgc ggcatttacc tgacagcgag
    21661 gtcatctcta tttatgatgc cgcgatgcgc tataagcagg agcaaacgcc gctggcggtg
    21721 attgccggga aagagtatgg atcaggctcc agccgtgact gggcggcaaa aggtccgcgt
    21781 ctgcttggta ttcgtgttgt gattgccgaa tcgtttgaac gaattcaccg ctcgaattta
    21841 attggcatgg gcatcctgcc gctggaattt ccgcaaggcg tgacgcgtaa aacgttagag
    21901 ctaaccgggg aagagaagat tgatattgtc gatctgcaaa atctacaacc cggcgcgacg
    21961 gttccggtga cgtttacgcg cgcggatggt agccaggaag tcgtaccctg ccgttgtcgt
    22021 atcgacaccg cgacggagtt gacctactac cagaacgacg gcattttgca ttatgtcatt
    22081 cgtaatatgt tgaagtaacg agtatttgct tgccggttat tttgcttccg gcaagcaaat
    22141 gaattacaca atgcaagagg gttatttgtt cagcaaatga cccattttct cggctttggt
    22201 atcgagataa tgttcgttat tggggttacg acctacaatc aacggtacgc gttcaacaat
    22261 attaatccct gcttcggtca gaatttcgac ttttttcggg ttgttggtta acaagcggac
    22321 ttcattgacg ccaaggagtt tgaacatatc agcgcaaaga gtgaagtcgc gctcatcagc
    22381 ggcgaagcct aactggtggt tagcctctac agtatcgtaa ccttgatcct gcagtgcata
    22441 agcgcggatt ttattcagca gaccaatgtt acgaccttcc tgacggtgat acagcaaaat
    22501 accacggcct tcttcggcaa tttgcgtcag tgccgcttcg agctggaagc cacaatcgca
    22561 gcgcaagctg aacagggcgt caccggtcag acattcggaa tggacgcgcg caagtaccgg
    22621 ggtatgcccg gaaatatcgc catagactag cgcgacatga tcgtgtccgg ttgccagttc
    22681 ttcaaatccc accatcagga aatcgcccca tggggttggc agtttggctt ctgccacacg
    22741 tttaagctgc atgaaattct ccagataatg ctggttctgt attggcttat tttgccataa
    22801 cgagaagggg ttcacctaat cacgacgcgt cgatcgttca cggaatggca caaatctgtc
    22861 aatttttcct ggaactggca ttttcagtta tgattgtggg acttatcaaa aaggagaggc
    22921 catgcgttcg attgccagac gtaccgcagt gggagctgca ctattgcttg ttatgccagt
    22981 agccgtatgg atatctggct ggcgttggca acctggagaa caaagttggc tactaaaagc
    23041 ggctttttgg gttactgaaa ctgtcaccca gccctggggc gtcattacac atttgatttt
    23101 attcggttgg tttctctggt gtctgcgttt tcgcattaag gctgccatta tgttatttgc
    23161 cattctggcg gcggcaatcc ttgtgggaca aggcgttaaa tcctggatca aagacaaagt
    23221 ccaggaacca cgaccttttg ttatctggct ggaaaaaaca catcatattc cggttgatga
    23281 gttctacact ttaaagcgag cagaacgcgg aaatttagtg aaagaacagt tggctgaaga
    23341 gaataatatc ccacaatatt tgcgttcaca ctggcagaaa gagacggggt ttgcctttcc
    23401 ttccggtcac acgatgtttg ctgccagttg ggcgctgctg gccgttggtt tgctgtggcc
    23461 gcgtcggcga acgttaacta ttgctatctt gctggtctgg gcaaccggag tcatgggaag
    23521 ccgcctgctg ctcgggatgc attggccacg cgatctggtg gtagctacgt taatttcgtg
    23581 ggcgctggtg gcggtggcaa cgtggcttgc gcaacgagtt tgtgggccat taacaccacc
    23641 tgcggaagaa aatcgcgaaa ttgcacaacg agaacaagaa agttaacgct ggttgatttt
    23701 ccgaatttag cccttaaatc atcaacaatg cgcgtggatg ccatttcgca gacggcgcga
    23761 aaatggtact ttaaagggct attgcggtaa gttgaccata atttattcgc tctaaccaca
    23821 taacgggaag taatgtgaaa tatttactca ttttcttact ggtgttagcg atcttcgtga
    23881 tttcggtcac gttgggtgcg cagaacgatc aacaggtgac gtttaattat ctgttagcgc
    23941 aaggggagta ccgtatttcc acattgctgg cggtattgtt tgctgcgggg tttgctatcg
    24001 gttggctgat ttgcggcctg ttctggctgc gagttcgtgt ttccctggcg cgcgctgaac
    24061 gtaaaataaa gcgactggaa aaccagcttt cacccgcgac tgacgtggct gtagcgccgc
    24121 attcgtcagc ggcgaaggaa taactttcta tgctggagtt gttgtttctg cttttgcctg
    24181 tagccgctgc ctatggctgg tatatgggcc gcagaagtgc gcaacaaaac aagcaagatg
    24241 aagccaaccg cttgtcgcgt gattacgtag cgggggttaa cttcctgctt agtaatcaac
    24301 aggataaagc ggtagacctg tttctcgata tgcttaaaga ggatacgggt accgttgaag
    24361 cccaccttac gctcggaaac ctgttccgtt cgcgtggcga agttgatcgc gctattcgca
    24421 tccatcagac cctaatggaa agcgcctcgc tgacctatga acagcgtctg ttggcgattc
    24481 aacaactggg gcgtgattac atggccgccg ggttatacga ccgcgcggaa gacatgttca
    24541 atcaactgac cgatgaaact gacttccgca ttggcgcgct gcaacagttg ctacaaatct
    24601 accaggctac cagcgagtgg cagaaagcaa ttgatgttgc cgaacgcctg gtgaagctgg
    24661 gtaaagataa gcagcgcgtc gaaattgctc atttctactg cgagttagcc ctacagcata
    24721 tggccagcga cgatctcgat cgtgccatgg ccttgttaaa aaaaggtgcg gcggcagata
    24781 aaaacagcgc ccgcgtatcc atcatgatgg gacgcgtatt tatggcgaaa ggagaatacg
    24841 ccaaagccgt cgaaagtttg caacgcgtga tatcccagga cagagaactg gtcagcgaaa
    24901 cgctggaaat gctgcaaacc tgctaccagc agttgggtaa aactgccgaa tgggcagaat
    24961 tcctgcagcg cgcggtggaa gagaacaccg gtgccgatgc tgaactaatg cttgcggaca
    25021 tcatcgaagc gcgcgacggt agtgaggccg cacaggtcta tattacgcgt cagcttcagc
    25081 gtcatccgac catgcgtgtg ttccataagc taatggatta ccacttaaat gaagcggaag
    25141 aagggcgtgc caaagagagt ctgatggtgc tgcgggacat ggttggcgag aaggtgcgta
    25201 gtaagcctcg ttatcgctgc cagaaatgtg gttttaccgc atataccctc tactggcatt
    25261 gtccgtcttg tcgggcctgg tcaaccatta aaccgattcg cggtcttgat ggcctgtaat
    25321 ttttaaaaaa atccgacttt agttacaaca tactaattat tgatgttcca ttgtgctccg
    25381 gtaactcggg cgcagaaaag cctgccaggg gagaaatcgc aactgttaat tttttatttc
    25441 cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc atcatcaaga
    25501 aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga attctcctgt
    25561 ggttgttgcc cttgattatc ataatcgtga tgccgcgctg gcctttgtcg acaagatcga
    25621 cccacgcgat tgtcgtctga aagtcggcaa agagatgttt acgctgtttg gacctcaatt
    25681 tgtacgcgaa cttcaacagc gtggttttga tatcttcctc gacctgaaat ttcacgatat
    25741 tcccaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt ggatggtgaa
    25801 tgttcacgcc tccggtgggg cgcgtatgat gaccgcagcg cgtgaggcac tggttccgtt
    25861 tggcaaagat gcaccgcttt tgattgctgt gacagtattg accagcatgg aagccagcga
    25921 cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc tggcagcact
    25981 gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg tgcgctttaa
    26041 acaggtattt ggtcaggagt tcaaactggt tacaccgggc attcgtccgc aggggagtga
    26101 agctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg gtgttgatta
    26161 tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcagacgc tgaaagcgat
    26221 caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct ggtctactca
    26281 acggaaaccg gacgtattga tgagccaaaa gcggcccctg tacgccctaa aggcgacggt
    26341 gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg cttgattacc
    26401 ggtgtcgatc tcgatgatgc cgaactgaca aaactggcag cggaactgaa gaaaaaatgc
    26461 ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa gcgtgattta
    26521 ttaaaatcac tgcttgaagc gaaagggatg aaggtaaaac tcgcaggcgg ttaacataaa
    26581 aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt cagttatttc
    26641 gtactgaaca gaatcaagcg tattataatt gcgagtcgat aatcgacatt tatttacgat
    26701 tatttaccga cctggtgacc aataacacca ccgacagctg cgccacctaa tgtacccagc
    26761 gtactgccat cggtcagtac tgcaccgcct aatgcacctg cacccgcgcc aattgcggtg
    26821 ttacggtctc gtttagacca gttagaacag gcactcagag acattgccag ggtaattgcc
    26881 agaacagctg cggtcatttt tttgctcgtt acaaacataa tactctctcc tgaatttatg
    26941 attcacggaa gtaagctctc tataactata atagctaaga ataagccggg tgaaattatt
    27001 ccgtgaaatc cgctcgcgcg cagggttata tcacgcaggt gataatgact tcctgttata
    27061 tcgctgataa taattttata tcttgagagt gttaataaca ggtaaatagt cttaattatc
    27121 aaccaggaat catcttagag cggatgattt gccaaagtgc aaatcatcca tagagaaggg
    27181 aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat gcatcagatc
    27241 gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg ggccgatgga
    27301 gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcgctt cgcactcttt
    27361 ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtaa acccagtttc
    27421 aggttgccag ccatcaatac caataaatgc tttgctaaaa tgcacctgtt ggatgcactg
    27481 gcgtgtcaaa gggccaacca tactttcgct ttttttctgg tacacgccac cgagcaaaat
    27541 aacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg tgatgatcgt
    27601 gacatttttc ttctgctcgc ccagagtccg ggcgagcagg gcattgctgc tgccattttc
    27661 gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga gttcacgctt
    27721 cagcgtatag ttgctcatca tacgagtttc gacgtcatca ctatcaagcg atactgcaaa
    27781 gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct ggcgaatggt
    27841 aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac cctggtcaat
    27901 gaccatctgt agaatagttt gttgtcggga attcatcgct gta
//
LOCUS       DAYPHN010000051        27632 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000051 DAYPHN010000000
VERSION     DAYPHN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.051"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(142..1806)
                     /gene="glnS"
                     /locus_tag="ACG0BW_002098"
     CDS             complement(142..1806)
                     /gene="glnS"
                     /locus_tag="ACG0BW_002098"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFK7259062.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2009..3955)
                     /gene="nagE"
                     /locus_tag="ACG0BW_002099"
     CDS             complement(2009..3955)
                     /gene="nagE"
                     /locus_tag="ACG0BW_002099"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFK7259063.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAIPNAVSVAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNENARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4288..5088
                     /gene="nagB"
                     /locus_tag="ACG0BW_002100"
     CDS             4288..5088
                     /gene="nagB"
                     /locus_tag="ACG0BW_002100"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFK7259064.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5148..6296
                     /gene="nagA"
                     /locus_tag="ACG0BW_002101"
     CDS             5148..6296
                     /gene="nagA"
                     /locus_tag="ACG0BW_002101"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFK7259065.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            6305..7525
                     /gene="nagC"
                     /locus_tag="ACG0BW_002102"
                     /gene_synonym="nagR"
     CDS             6305..7525
                     /gene="nagC"
                     /locus_tag="ACG0BW_002102"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFK7259066.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7573..8325
                     /gene="nagD"
                     /locus_tag="ACG0BW_002103"
     CDS             7573..8325
                     /gene="nagD"
                     /locus_tag="ACG0BW_002103"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFK7259067.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8376..8474)
                     /locus_tag="ACG0BW_002104"
                     /pseudo
     CDS             complement(8376..8474)
                     /locus_tag="ACG0BW_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8722..10386
                     /gene="asnB"
                     /locus_tag="ACG0BW_002105"
     CDS             8722..10386
                     /gene="asnB"
                     /locus_tag="ACG0BW_002105"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFK7259068.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(10428..10864)
                     /locus_tag="ACG0BW_002106"
                     /pseudo
     CDS             complement(10428..10864)
                     /locus_tag="ACG0BW_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11198..11274
                     /locus_tag="ACG0BW_002107"
     tRNA            11198..11274
                     /locus_tag="ACG0BW_002107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11232..11234,aa:Met,seq:cat)
     gene            11284..11368
                     /locus_tag="ACG0BW_002108"
     tRNA            11284..11368
                     /locus_tag="ACG0BW_002108"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11318..11320,aa:Leu,seq:tag)
     gene            11392..11466
                     /locus_tag="ACG0BW_002109"
     tRNA            11392..11466
                     /locus_tag="ACG0BW_002109"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11424..11426,aa:Gln,seq:ttg)
     gene            11501..11575
                     /locus_tag="ACG0BW_002110"
     tRNA            11501..11575
                     /locus_tag="ACG0BW_002110"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11533..11535,aa:Gln,seq:ttg)
     gene            11591..11667
                     /locus_tag="ACG0BW_002111"
     tRNA            11591..11667
                     /locus_tag="ACG0BW_002111"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11625..11627,aa:Met,seq:cat)
     gene            11716..11790
                     /locus_tag="ACG0BW_002112"
     tRNA            11716..11790
                     /locus_tag="ACG0BW_002112"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11748..11750,aa:Gln,seq:ctg)
     gene            11828..11902
                     /locus_tag="ACG0BW_002113"
     tRNA            11828..11902
                     /locus_tag="ACG0BW_002113"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11860..11862,aa:Gln,seq:ctg)
     gene            complement(12056..13231)
                     /gene="ubiF"
                     /locus_tag="ACG0BW_002114"
     CDS             complement(12056..13231)
                     /gene="ubiF"
                     /locus_tag="ACG0BW_002114"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFK7259069.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSHGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGMLKRQALKYALGL"
     gene            13377..14801
                     /gene="miaB"
                     /locus_tag="ACG0BW_002115"
     CDS             13377..14801
                     /gene="miaB"
                     /locus_tag="ACG0BW_002115"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFK7259070.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14954..15994
                     /gene="ybeZ"
                     /locus_tag="ACG0BW_002116"
     CDS             14954..15994
                     /gene="ybeZ"
                     /locus_tag="ACG0BW_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFK7259071.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15991..16458
                     /gene="ybeY"
                     /locus_tag="ACG0BW_002117"
     CDS             15991..16458
                     /gene="ybeY"
                     /locus_tag="ACG0BW_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFK7259072.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLIYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16548..17426
                     /gene="corC"
                     /locus_tag="ACG0BW_002118"
     CDS             16548..17426
                     /gene="corC"
                     /locus_tag="ACG0BW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFK7259073.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17451..18989
                     /gene="lnt"
                     /locus_tag="ACG0BW_002119"
     CDS             17451..18989
                     /gene="lnt"
                     /locus_tag="ACG0BW_002119"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFK7259074.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            19386..20294
                     /gene="gltI"
                     /locus_tag="ACG0BW_002120"
     CDS             19386..20294
                     /gene="gltI"
                     /locus_tag="ACG0BW_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFK7259075.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            20464..21204
                     /gene="gltJ"
                     /locus_tag="ACG0BW_002121"
     CDS             20464..21204
                     /gene="gltJ"
                     /locus_tag="ACG0BW_002121"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFK7259076.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     EMDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            21204..21878
                     /gene="gltK"
                     /locus_tag="ACG0BW_002122"
     CDS             21204..21878
                     /gene="gltK"
                     /locus_tag="ACG0BW_002122"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFK7259077.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITITAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKTDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21878..22603
                     /gene="gltL"
                     /locus_tag="ACG0BW_002123"
     CDS             21878..22603
                     /gene="gltL"
                     /locus_tag="ACG0BW_002123"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFK7259078.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22721..23656
                     /gene="rihA"
                     /locus_tag="ACG0BW_002124"
     CDS             22721..23656
                     /gene="rihA"
                     /locus_tag="ACG0BW_002124"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFK7259079.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNASVMVDVDRQGFVDLLADRLKFYA"
     gene            23740..25410
                     /gene="hscC"
                     /locus_tag="ACG0BW_002125"
     CDS             23740..25410
                     /gene="hscC"
                     /locus_tag="ACG0BW_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFK7259080.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     AVAKNMLNESELAALYTSVEAAKCGNQLPLQISWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(25470..26921)
                     /gene="djlC"
                     /locus_tag="ACG0BW_002126"
     CDS             complement(25470..26921)
                     /gene="djlC"
                     /locus_tag="ACG0BW_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFK7259081.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADNEGKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIESRYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFMAMEGPWFIPDDVKL
                     HRKLLRWYSSVKIGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEAQSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENPEEKND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26918..27625)
                     /gene="ybeU"
                     /locus_tag="ACG0BW_002127"
     CDS             complement(26918..27625)
                     /gene="ybeU"
                     /locus_tag="ACG0BW_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFK7259082.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEDESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6763 a         7188 c         7050 g         6631 t
ORIGIN      
        1 attttatgga taaattgcga agcggaatta attcgcgaag aaaagcagaa aaaacgccgc
       61 agagcgcggc attttgaatt gtttgcatgt ttcatgccgg atgcggcgta aacgcctcac
      121 ccggcctggt agaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac
      181 ggtgcggtta aataccggtt tttccgccgt agaatggcgg ctgtcgaggc agaaataacc
      241 ttcacgctca aactggaatg ctttacccgc taccgcgtct ttcagagacg gttcagcaaa
      301 gccctgtttg atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc
      361 tgggttcggc acgctgaaca gacgatcgta caagcggatt tcaaccggca gcgcatgtgc
      421 cgcgctcacc cagtggataa cacctttcac tttacgacca tctgccggat ctttgcttaa
      481 ggtgtcggcg tcataagtac agaagatggt ggtgatattg ccttcggcat ctttctccac
      541 gcgttctgcc ttaatgacgt aagcattacg cagacgcact tctttaccca gcaccagacg
      601 cttgtactgc ttgttagctt cttcgcggaa atcggcgcgg tcgatccaaa tctcaccgct
      661 aaacggcacc tgacggctcc ccatttccgg tttgttcgga tggttcggca tggtaaccat
      721 ttcgccttcg ccctggtagt tttcgataac cagtttcacc ggatcgataa ccgccattgc
      781 gcgcggggcg ttttcgttga gatcttcacg gatgcaggat tccagcgacg ccatttcaat
      841 ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagccgcagt
      901 gtaaccacga cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac
      961 gtgcttgtcg gtcaccagca ggttcagctt acgcttggac atcacagtgt attccagatt
     1021 caggcgcgag aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca
     1081 gtcgtacagg cgacggttgt cctggaactc aagcgtacac agagagtgcg taataccttc
     1141 cagcgcatcg ctgatgcaat gggtgaagtc gtacatcggg tagatgcacc acttgttgcc
     1201 agtctggtgg tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca ttacgataaa
     1261 tggcgacgcc atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccaccggt
     1321 acgcattttt tcgaacagcg ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt
     1381 tttacccggc tgcgtcaggg tgccgcggta ttcgcggatc tgttccggtg tcagttcatc
     1441 aacgtacgcc aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata
     1501 atcggaggag taacggacgt taccagacca gtgaaaacct aaccactcta cgtcgttttt
     1561 gatcgactca acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt
     1621 gcactggcct ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc
     1681 aatatgcaga tagccattcg gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc
     1741 actggccaga tcttcatcga tgatctgacg aataaagtta gtcgggcggg cttctgcctc
     1801 actcatcgtg gattcctcaa agcgtaaaca acgtataacg gcgtatgatc ttataagcgg
     1861 gacaggctga caactgttag ttagtgaaaa tcgaaaatct cactgagatt atggggatga
     1921 ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt gtcattgttg gatgcgacgc
     1981 tgccgtgtcg catcaggtat aaagcagatt actttttgat ttcatacagc ggtgtttgac
     2041 ccgccacgac atggccctga gctttaatga tcaagccgct gaaatcgtcg atattgctgc
     2101 aaaccaccgg gctaatcatc gagcgggcgt tttcgttcag ataatccaga tccatttcca
     2161 gaatcggttg ccctgcgctt acttgcgcgc cctcttccac cagacgttta aagcctttac
     2221 cttccagcgc tacggtgtcg atacccatat ggacgacgat ctccgcgcct ttttcggttt
     2281 ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg gctggtgata
     2341 cgacgatttt atctgtcggt ttcaccgcca caccgtcacc caccgctttg ctggcgaatg
     2401 cttcgtcagg aacctgatcc agtgccacaa catcaccggt aatcggcgat accagctccg
     2461 cgacagacac cgcgtttggt atagcctgcg gttttgctac aggcgcggca gttgcaggcg
     2521 cagtttcagc tgacgcagcg gctaccggac cacgggcaac aactttcttc attgcatcgc
     2581 caatggattc cgctttcgcg ccaacaatca cctgaatagt ctgtttgttc agtttcacta
     2641 ccccagaagc acccagccgt ttacacatcg catcgttgac gcgagccgag tcaaccacgg
     2701 tcaggcgcag acgagtaata caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg
     2761 cagcaatata gttggtcgcc agttgattga ggccttcttc agtgttgctg ttggcttctt
     2821 cagtaacgat ctcatcttct ttatcttcac gacccggcgt tttcaggttg aacatgcgga
     2881 taaccaaact gaacaccacg aagtagatag cgaagaagac aacacccata accagcagca
     2941 tccagacgtt ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg atagcccccg
     3001 cagagaagga gaagcccgcg tggataccca gcagcgttgc cacaaacagg ctgataccgg
     3061 tcagcagtgc gtgcaggagg tacagcagcg gcgcaaggaa catgaacagg aattccagcg
     3121 gctcagtcac accggtcagg aacgcagtaa cagcaacaga aagcagcata ccgccaacca
     3181 tcggacgacg ctctttcggt gctgcaaagt acatcgccag cgccgcaccc ggcagaccga
     3241 acatcatgat cgggaagaag ccggacatga acatccccgc ggtgccgtca ccggcataga
     3301 agcggttgat gtcaccgtgg aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga
     3361 accaggcgat ggtgttcagt acctgatgca gaccggttgg gatcagcaga cggttgatga
     3421 aaccaaagat accggaaccc agcgcgcccg cagaaacgat ccactcgccg cctgcatgga
     3481 tagcgtgctg taccggcggc cagacgtaac caaaaatggc cgccagcacc aggcagaaga
     3541 agccggtggc gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt
     3601 taatatcgga ccaacggtta taggctgcac caccgaccag accggtaatg atacccgcca
     3661 gtacacccat gttaatttcc gggttgatgg tcaccatcgc tttggttaac acaaagtaac
     3721 ctaccgcacc cgccagtgcc gccgcacctg cgctgtcttt cgaccagctg gatgccacgc
     3781 cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg
     3841 caacgttaag taaatctggc tgaccgaatc gcagcaacag cgccgccacc ggcagcaccg
     3901 cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata ttcatcttat
     3961 tccccctacg agaaccctat ttggctcgtt taaagccgta tttttatttt gctgcaaatt
     4021 gtactgccga tgttctgtaa tcagattgtt agatcatctg ctacagagtg tgtgaaaatt
     4081 taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat
     4141 catcactccc ttttactggc taaaccagaa aacttatttt atcattcaaa aaatcaggtc
     4201 ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg
     4261 cttacatttt acttattgag gtgaataatg agactgatcc ccctgactac cgctgaacag
     4321 gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa accgactgcc
     4381 gatcgtccgt ttgttctggg cctgccgact ggcggcacgc cgatgaccac ctataaagcg
     4441 ttagtcgaaa tgcataaagc aggccaggtc agctttaagc acgttgtcac cttcaacatg
     4501 gacgaatatg tcggtctgcc gaaagagcat ccggaaagct actacagctt tatgcaccgt
     4561 aatttcttcg atcacgttga tattccagca gaaaacatca accttctcaa cggcaacgcc
     4621 ccggatatcg acgccgagtg ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt
     4681 catctgttta tgggcggtgt aggtaacgac ggccatattg catttaacga accggcgtct
     4741 tctctggctt ctcgtactcg tatcaaaaca ctgactcatg acactcgcgt cgcgaactct
     4801 cgtttctttg ataacgatgt caatcaggtg ccaaaatatg ccctgactgt cggtgttggt
     4861 acattgctgg atgccgaaga agtgatgatt ctggtgctgg gtagccagaa agcactggca
     4921 ctgcaggccg ccgttgaagg ttgcgtaaac cacatgtgga ccatcagctg tctgcaactg
     4981 catccaaaag cgatcatggt gtgcgatgaa ccttccacca tggagctgaa agttaagact
     5041 ttaagatatt tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt gttatccctg
     5101 ccctgacacc ttgctcaggg caatattttt taaaatcggg ggtcagaatg tatgcattaa
     5161 cccagggccg gatctttacc ggccacgaat ttcttgatga ccacgcggtt gttatcgctg
     5221 atggcctgat taaaagcgtc tgtccggtag cggaactgcc gccagagatc gaacaacgtt
     5281 cactgaacgg ggccattctc tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg
     5341 gcgtgcagtt taacgatact gctgaagcag tcagcgtaga aacgctggaa atcatgcaga
     5401 aagccaatga gaaatcaggc tgtactaact atttgccgac gcttatcacc accagcgacg
     5461 agctgatgaa acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat ccgaatcagg
     5521 cgttaggtct gcatctggaa ggcccgtggc tgaatctggt aaaaaaaggc acccataatc
     5581 cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt cctgtgcgaa aatgccgatg
     5641 tcattaccaa agtgaccctg gcaccggaaa tggttcctgc agaagtcatc agcaaactgg
     5701 caaatgccgg gattgtggtt tctgccggtc actccaacgc gacgttgaaa gaagcgaaag
     5761 ccggtttccg cgcggggatt acctttgcca cccatctgta caacgcaatg ccgtatatta
     5821 ccggtcgtga gccgggcctg gcgggcgcga tcctcgacga agctgacatt tattgcggta
     5881 ttatcgctga tggcctgcat gttgattacg ccaacattcg caacgctaaa cgtctgaaag
     5941 gcgacaaact gtgtctggtt actgacgcca ccgcgccagc aggtgccaac attgaacagt
     6001 tcatttttgc gggtaaaaca atatactacc gtaatggact ttgtgtggat gagaacggta
     6061 cgttaagcgg ttcatcctta accatgattg aaggcgtgcg taatctggtc gaacattgcg
     6121 gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg
     6181 ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc caacctgact gcattcacac
     6241 ctgattttaa aatcaccagg accatcgtta acggtaacga ggtcgtaact caataagaga
     6301 aagtatgaca ccaggcggac aagctcagat aggtaatgtt gatctcgtaa aacagcttaa
     6361 cagcgcggcg gtttatcgcc tgattgacca gtatgggcca atctcgcgga ttcagattgc
     6421 cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg
     6481 cgggctgatt aaagaagttg atcagcaggc ctccaccggg ggccgccgcg ctatctctat
     6541 cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc acgacgccac
     6601 catcaccctg tttgatctca gcagcaaagt gctggcagaa gaacattacc cgttgccgga
     6661 acgtacccag cagacgctgg aacatgccct gctgaatgcc attgctcagt ttattgatag
     6721 ctaccagcgc aagctgcgcg agctgatcgc catttccgtg atcctgccgg ggcttgttga
     6781 cccggacagc ggcaaaattc attacatgcc gcatattcag gtggaaaact gggggcttgt
     6841 agaagcactg gaagagcgtt ttaaagtgac ctgttttgtc ggccacgata tccgcagtct
     6901 ggcgctggcg gagcactact tcggtgcaag tcaggattgc gaagactcca ttttagtgcg
     6961 tgtccatcgc ggaaccgggg ccgggattat ctctaacggg cgcattttta ttggccgcaa
     7021 cggcaacgtc ggtgaaattg gccatatcca ggtcgaaccg ctgggtgaac gctgccactg
     7081 tggcaacttt ggctgcctgg aaactatcgc cgccaacgct gccattgaac aacgggtgtt
     7141 gaatctgtta aagcagggct accagagccg cgtgccgctg gacgactgca ccataaaaac
     7201 tatctgcaaa gctgcgaaca aaggcgatag cctggcgtcg gaagtgattg agtatgtcgg
     7261 tcgtcatctg ggcaaaacca tcgccattgc catcaacctg tttaacccgc aaaaaattgt
     7321 tattgccgga gaaatcaccg aagccgataa agtgctgctc cctgctattg aaagctgcat
     7381 taatacccag gcgctgaagg cgtttcgcac taatctgccg gtggtacgtt ctgagctgga
     7441 tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca acggtatttt
     7501 gctccagcat ttgctggaaa attaatgtgc ttttatagtg gcgcttattg ttgtcaatat
     7561 tctgggtagt ccatgaccat taaaaatgta atttgcgata tcgacggcgt gctgatgcac
     7621 gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg ggattatgga taaaggcctg
     7681 ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc aagatctggc gaaccgcttt
     7741 gccactgccg gtgtcgatgt acctgacagc gtgttttata cctctgcgat ggcgaccgcc
     7801 gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt
     7861 catgaactgt acaaagccgg tttcactatt accgatgtga accctgattt tgtgattgtt
     7921 ggcgaaacgc gttcctacaa ctgggacatg atgcataaag cagcctattt cgtcgctaac
     7981 ggtgcacgtt ttatcgccac caacccggac acccacgggc gcggttttta tccggcgtgt
     8041 ggtgcgctgt gtgcggggat cgaaaaaatc tccggacgta aaccgttcta tgttggtaag
     8101 ccgagtccgt ggatcatccg cgcagcatta aacaaaatgc aggcgcattc ggaagaaacg
     8161 gtgattgtcg gcgataacct gcgtaccgat attctggcgg gcttccaggc cggtctggag
     8221 acgattctgg tactctccgg cgtttcgtcg ctcgacgata tcgacagtat gccattccgc
     8281 cccagctgga tttacccatc ggtcgccgaa attgacgtta tctgaatacc acccacgtct
     8341 gcggacgtgg ttggctctga tgacaagcgc caaactgcct tatgcgctac gcttatcagg
     8401 cctacgcagc tcctgcaata tattgaattt tcatgctttt gtaggccgga taaggcgttc
     8461 acgccgcatc cggcatgaac aaatcgcact ttgtcagcaa tttggccccc gtaaaatata
     8521 accattcaat aaaacccgct aaaaattatc gattcatcaa taaatacatc cttacaccag
     8581 accttttttc accaatcgac aatcaccatt acgtttttta ttttttcacc gccaaatcgc
     8641 ttgcgcacag tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat
     8701 accaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac
     8761 gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg
     8821 gactggtccg gtatttatgc cagcgataac gccattctcg cccacgaacg tctgtccatt
     8881 gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaactca tgtgctggca
     8941 gtaaacggtg aaatctacaa ccaccaggca ttgcgcgctg aatatggcga tcgttaccag
     9001 ttccagaccg ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa
     9061 tttctcgacg acttacaggg catgtttgcc tttgccctgt acgacagcga aaaagatgcc
     9121 tacctgattg gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgaacat
     9181 ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc cggtatgccg cacgattaaa
     9241 gagttcccgg cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat
     9301 cgcgactggt tcgactacga tgcggtgaaa gataacgtga ccgacaaaaa cgagctgcgt
     9361 caggcactgg aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgctg
     9421 ctatctggtg gcctggattc ctcaatcatt tccgctatca ccaagaaata cgcagcccgt
     9481 cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc agctgcactc ctttgctgta
     9541 ggtctgccgg gttcaccgga tctgaaggca gcccaggaag tggcaaacca tctgggcact
     9601 gtgcatcatg aaattcactt cactgtacag gaaggtctgg acgccatccg cgacgtgatt
     9661 taccacatcg aaacctatga tgtgaccact attcgcgctt caacaccgat gtatttaatg
     9721 tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg atctgatgaa
     9781 gtgtttggcg gttatcttta cttccacaaa gcgccgaatg ccaaagaact gcatgaagag
     9841 acggtgcgta aactgctggc cctgcatatg tatgactgcg cccgtgccaa caaagcgatg
     9901 tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct cgacgtggcg
     9961 atgcgcatta acccacagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg
    10021 cgtgaatgtt ttgaagcgta tctgcctgca agcgtggcct ggcggcagaa agagcagttc
    10081 tccgatggcg tcggttacag ttggatcgat accctgaaag aagtggcggc gcagcaggtt
    10141 tctgatcaac agctggaaac tgcccgcttc cgcttcccgt acaacacacc gacctcaaaa
    10201 gaagcgtatc tgtaccggga gatctttgaa gaactcttcc cgcttccgag cgccgctgag
    10261 tgcgtgccgg gcggcccgtc cgtcgcgtgt tcttccgcta aagcgattga gtgggatgag
    10321 gcgttcaaga aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat
    10381 aagtaagaaa tgcggtactg ccccggagaa atccggggct gttttagtta ttgagctgct
    10441 tgttgattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac
    10501 tcatcactgt aggccaccga ccaggtcgcc tgctcatccg gaatatgcag gctgccattc
    10561 ccctctttca ggcgtaataa ctcaaaagat ttgcccttaa ttacggcggt ataggatttt
    10621 caccttcctg taatgctttc actttacagc gaaaaatatg gccatcgatg tcattatcaa
    10681 catgcgatac ctgaatgagg gcttcattag aggctttcgg ccctatccgc atcatccaca
    10741 ccacagcgcc ttcctgccca cgatagccaa aaacatattt accgatttcc ggtttgaccg
    10801 tatcggcaaa cgcagattgc ataagtaata acgcacccat cagcgccgtc ataaaacgca
    10861 gcatagtatt ccctcaataa atcgcctgaa gtggtagcag agatgcaggc gtagcgatcc
    10921 ctgccctgtc ctgttgcaac gggccagtat ttcatagatt aatttcccac cactattgtt
    10981 gaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggga aaaaacacaa
    11041 agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact
    11101 ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc
    11161 cccgcaacgc cgataaggta tcgcgaaaaa aaaagatggc tacgtagctc agttggttag
    11221 agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accatctttt
    11281 tttgcgggag tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt
    11341 gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc
    11401 gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc
    11461 cagccatctt cttcgagtaa gcggttcacc gcccggttat tggggtatcg ccaagcggta
    11521 aggcaccggt ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcgaa
    11581 gaaacaatct ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    11641 caggttcgaa tcccgtcgta gccaccaagt tctgaatgtg tcgaatgcgt tcggcaaatt
    11701 cacaaaccaa tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
    11761 tccgaggttc gaatcctcgt accccagcca atttattcaa gacgcttacc ttgtaagtgc
    11821 acccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
    11881 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
    11941 tgcgtatatt caatgtcgaa tgcgatgttg actcgtctta tccttcaaag tcggatgcga
    12001 cgctgacgcg tcttatccga cctacggttg gcatgcatcc ggcaatgttg taaggctaca
    12061 accctaacgc atatttcagc gcctgacgtt tcaacatgcc agcacgctcc gccgccatca
    12121 aaccaagatt acgcacaaaa cgcagtggtg gcagattatt gctgaatccg gcataaaaca
    12181 gatccatacc gctctgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
    12241 ggatcggata actggcccac gcttcgccat ggctgcgagc gttgaccaga acatcaatca
    12301 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
    12361 gcgcggcatc gcccaccagt gccagtcccg gctgcacata ctgcaacgca tggcggcgcg
    12421 tcagcggaaa agcgccagcg gcaagcggtg ttacataccc aagacgtgac gggaaatgct
    12481 tcgcgatttc cgcctgtagc tgcgccatat tcatattctg caactggcga atgcgtgccg
    12541 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgaggtc
    12601 catccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
    12661 tcaacataca cgactgtgca tactgccagg catgaacgcc aattcccgcc atctgccgca
    12721 cctgcgaatt tgcgccgtcg gcaccaatca ccagcttcgc gcggatcgtt tcaccgccct
    12781 tcagttccag ctcctgaaga tcattatggc gatgcagcgc aatcagcgag cctggcacac
    12841 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacagtgt
    12901 tttccaccat gtagccaagc agcggtagct taagttcagc ggcgtcaaac accacatgcg
    12961 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtaccg
    13021 cgtcccagac gcctaaacct ttaagcaatg ataccgaagc cgcactgatc gccgagatcc
    13081 gcacgtccgg ttggctgtca gcgacaaacg gcgcaggttc tgcgtgctcg atcaccgtta
    13141 ccgcaaatcc gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt cctccgccga
    13201 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
    13261 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
    13321 aaagcattac actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
    13381 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
    13441 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
    13501 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
    13561 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggcg
    13621 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
    13681 tttttgggcc gcaaacgctg caccgcctgc cggagatgat caactccgtg cgcggcgacc
    13741 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctgccggaac
    13801 cgcgtgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
    13861 cctactgcgt agtgccttac acccgtggtg aagaggtaag ccgtccgtcc gacgatattc
    13921 tgtttgagat tgcccaactt gctgctcagg gcgtgcgtga agttaacctg ctcggccaga
    13981 acgtgaacgc ctggcgcggt gagaactacg acggcaccac cggatcgttt gccgatctac
    14041 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
    14101 tcgaattcac cgacgatatc atcgaagtgt accgcgacac gccggagctg gtgagcttcc
    14161 tgcatctgcc ggtacagagc ggttccgatc gcattctgaa cctgatggga cgtacccata
    14221 cagcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
    14281 tcagttctga cttcatcgtt ggcttccctg gtgaaaccac cgaagacttc gagaaaacga
    14341 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
    14401 cgggtacgcc agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc
    14461 tgtatattct acaagaacgt attaatcagc aagcgatggc atggagccgc cggatgctcg
    14521 gcaccaccca gcgtattctg gtagaaggta catcgcgtaa gagcatcatg gagctttccg
    14581 gtcgcactga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
    14641 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgaa
    14701 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
    14761 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
    14821 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
    14881 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
    14941 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
    15001 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aaacaactcg aacgccgtct
    15061 cggcatcgag atcaatcgcc gcgataacca ctttaaactg accggccgtc cgatttgcgt
    15121 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat accgccccga tgcgcggtca
    15181 gattcaggat attgaaccgg aacagatcca ccttgcgatt aaagaagcgc gggtactgga
    15241 gcaaagcgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaga ccaaacgcgg
    15301 cgtaatcaaa ccgcgcacgc caaaccaggc gcagtacatt gccaatattc tcgaccatga
    15361 catcaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
    15421 agttgatgcc ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc cggcggtaga
    15481 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtattt
    15541 gcgcccgctg tacgacgcgc tgtttgaaat gctgggcttt gagaaagtcg agaaactgat
    15601 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggccgta cgctgaacga
    15661 cgcgtttatc attctcgatg agagccagaa cactaccatc gaacagatga agatgttcct
    15721 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacgc agatcgacct
    15781 gccgcgtaat accaagtcag gcttacgtca tgccatcgaa gtgctggctg acgtcgaaga
    15841 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat
    15901 cgttaacgct tatgaagcct gggaagaagc agaacaaaaa cgtaaagcgg cgctggcagc
    15961 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
    16021 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcagacatg gctgaatgcg
    16081 gtgatcccgc agtttcagga agagtcggaa gtgactattc gcgtggtcga taccgccgaa
    16141 agccacagtc tgaatctgat ctatcgcggt aaggataagc cgaccaacgt gctctccttc
    16201 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
    16261 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
    16321 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
    16381 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
    16441 attgccgaga aagaataatt tgccaggcat ttgactggca gatgtccccg ccgttggtga
    16501 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
    16561 attcacatag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
    16621 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
    16681 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
    16741 cagaccaacg cgtccgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
    16801 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
    16861 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
    16921 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcagttgtcg
    16981 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
    17041 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
    17101 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgactttc
    17161 gtcagctgag tcgccatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg
    17221 aagcgttcgg cacccacttt agcgatgaag aggtcgacac catcggtggt ctggtgatgc
    17281 aggcatttgg gcatcttccg gcgcgtggcg aaaccatcga catcgacggt taccagttca
    17341 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
    17401 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
    17461 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
    17521 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ctgatggggc
    17581 ttcaggcgct gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
    17641 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acttttggcg
    17701 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
    17761 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
    17821 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtgctga
    17881 ccggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
    17941 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
    18001 cactggcgtt ggtcaaacgc aactggcgtc cgctggtagt ggccgtcgtg ctgtttgccc
    18061 ttcccttccc gctgcgttac atccagtggt ttaccccgca accggagaaa accattcagg
    18121 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggacgaaggc cagcttctta
    18181 atacgctgaa gatttactac aacgcaacgg caccgctgat gggcaaatca tcgttgatta
    18241 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
    18301 tggacggtga gttgcgcgat aaagggagct cgctggtaac cgggattgtt gacgcgcgtc
    18361 tcaataagca gaaccgctac gatacttaca acaccatcat cacgctgggg aaaggtgcgc
    18421 cgtacagcta cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt
    18481 ttgtcccgct ggagtcgatt ctgcgtccgt tagcgccgtt ctttgatctg ccaatgtcgt
    18541 cgttcagccg tgggccatat atccagccac cgctgtcggt aaacggtatt gagcttactg
    18601 cggctatttg ctacgagatc attctcggcg agcaagtgcg tgataacttc cgcccggata
    18661 ctgactacct gctgactatc tccaacgatg catggtttgg taagtctatt ggtccatggc
    18721 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccattg ctgcgtagca
    18781 ccaacaacgg cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc
    18841 agttcacccg cgaggtgtta accactaatg tgacaccgac caccggactc acaccgtatg
    18901 cacgtaccgg caactggccg ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgc
    18961 tgatgagcct gcgtcaacga cgtaaataat cccttcttat cgtgccggaa tacatcccgt
    19021 atttcggcac gctcaccgga tgcggcatca gtgcgttatc cggataacaa aattccccta
    19081 tccaactttg gcacatctat tgctttgtta tacaaggcaa agcttgaacc agcatcagta
    19141 caacccggtt gcaccacagc aatccacggg tagcaccaaa agagtgcatt ttaagaattt
    19201 tgcttcggaa tggtgcggcg catctcgcaa aaacaaacaa taaaacagca atatctttac
    19261 atttagccaa actaaatgtt acctaacagg cacaacactg cacaataaag ttgcagacga
    19321 taacaacaca aacactcaca acgggtatcc atgcgttctt aacgcagaag ataaaggagt
    19381 tggatatgca attacgtaaa cctgccacag caatcctcgc cctggcgctt tccgcaggac
    19441 tggcacaggc agatgacgcc gccccggcag cgggcagtac gctggacaaa atcgccaaaa
    19501 acggcgtgat tgtcgtcggt caccgtgaat cttcagtacc tttctcttat tacgacaatc
    19561 agcaaaaagt ggtgggttac tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa
    19621 agaaactcaa caaaccggac ttgcaggtga aactgattcc aatcacctca caaaaccgta
    19681 ttccactgct gcaaaacggc actttcgatt ttgaatgtgg ttccaccacc aacaacgtcg
    19741 aacgccagaa acaggcggct ttctctgaca ctattttcgt ggtcggtacg cgcctgttga
    19801 ccaaaaaggg gggcgatatc aaagattttg ccgacctgaa agacaaagcc gtagtcgtca
    19861 cttccggcac tacttctgaa gttttgctca acaaactgaa tgaagagcaa aaaatgaata
    19921 tgcgaattat cagcgccaaa gatcacggtg actctttccg taccctggaa agcggtcgtg
    19981 ccgttgcctt catgatggat gacgctctgc tggccggtga acgagcgaaa gcgaagaaac
    20041 cagacaactg ggaaatcgtc ggcaagccgc agtctcagga agcctacggt tgcatgttgc
    20101 gtaaagatga tccgcagttc aaaaagctga tggatgacac catcgctcag gtgcagacct
    20161 ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa tccaattccg ccgaaaaacc
    20221 tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca
    20281 aggcactgaa ctaattacaa gaaccagggg cggaaattcc agccctctcg attgttacgt
    20341 agcacggaca gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg
    20401 ctgggtaaca atcttcgagg gtagcagtta acgctgctac cctctttttt ctggagtaga
    20461 tttatgtcta tagactggaa ctggggtatt tttttacaac aagccccgtt cggcaacacc
    20521 acctatcttg gttggatctg gagtggtttt caggtgacga tcgctttgtc gatctgcgcc
    20581 tggattatcg ctttcctcgt cggttcattt ttcggcattt tacgtaccgt tccaaaccgt
    20641 tttctctccg gtctgggtac gttgtatgtc gagctgttcc gcaacgtgcc gttgattgtg
    20701 caattcttta cctggtatct ggtgatcccg gagctgttgc cggagaaaat cggcatgtgg
    20761 tttaaggccg agatggatcc caatattcag tttttccttt catccatgct ttgcctgggg
    20821 ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg cgattcagtc gttgccgcgt
    20881 gggcaaaaga atgccgcgct ggcaatgggg ctgacgctac cgcaggctta ccgctacgta
    20941 ctgctgccta atgcttatcg cgttatcgtc ccgccgatga cttcggagat gatgaacctg
    21001 gtgaaaaact ccgccatcgc ctcgactatc ggtctggtag atatggcggc gcaggcgggt
    21061 aaattactcg attactccgc tcacgcctgg gagtcgttta ccgccattac gcttgcgtat
    21121 gtgttgatta acgcttttat catgctggtg atgacgctgg ttgaacgtaa agttcgcctg
    21181 cccggcaaca tggggggcaa ataatgtacg agtttgactg gagttccatt gttccttccc
    21241 tgccatatct gctcgacggg ctggtgatca ccctgaaaat caccatcacg gcggtggtca
    21301 ttgggatttt gtggggcacg atgttggcgg taatgcgttt atccagcttt gcgcctgtgg
    21361 cgtggtttgc taaagcctac gttaacgtgt tccgttcgat cccactggtg atggtactgt
    21421 tgtggtttta cctgattgtg ccgggttttc tgcaaaatgt gctgggatta tcgccgaaaa
    21481 ccgatatccg tttaatttct gcaatggtgg cgttttcgat gtttgaagca gcctactatt
    21541 cagagattat ccgcgctggt attcaaagta tttctcgtgg tcagtcgagc gccgcgctgg
    21601 cgttggggat gactcactgg cagtcgatga aactgattat tctgccgcag gctttccgcg
    21661 cgatggtgcc gctgctgctc actcagggca tcgtactgtt ccaggatacc tcactggtgt
    21721 atgtgttatc tctggccgat ttcttccgta ccgcctcaac cattggtgag cgtgatggta
    21781 cgcaggttga gatgatcctg tttgccggat ttgtttattt cgttattagc cttagcgcgt
    21841 cgttgttggt cagctacttg aaaagaagga cagcataatg attaccctga aaaatgtttc
    21901 aaaatggtat ggtcactttc aggtgctgac cgactgctca accgaagtga aaaaaggcga
    21961 agtggtggtg gtttgcggcc cgtctggttc cggcaaatca acgctgatta aaaccgtcaa
    22021 cggcctcgaa ccggtgcagc aaggtgaaat taccgtcgat ggcatcgtgg ttaacgataa
    22081 gaaaaccgat ctggcaaagc tgcgttcccg cgtcgggatg gtgttccagc atttcgagtt
    22141 gttcccccat ttgtcgatta ttgaaaacct gaccctggcg caggtgaaag tgcttaaacg
    22201 cgataaagca cccgctcgtg aaaaagcatt gaaattgctg gagcgcgtgg gactttccgc
    22261 acatgccaat aaatttccgg cgcagctttc cggcggtcag caacagcgtg tggcgatcgc
    22321 tcgcgcattg tgtatggatc ctattgccat gctgttcgac gaaccgacat cggcgctgga
    22381 tccggagatg atcaacgaag tgctggacgt aatggtcgaa ctggcgaacg aaggaatgac
    22441 catgatggtg gtgacccacg aaatgggctt tgcccgtaaa gtggcgaatc gggtgatctt
    22501 tatggatgag ggtaaaattg tcgaagactc gccgaaagat gcgttcttcg atgatccgaa
    22561 atcggaccgc gcaaaagact tcctcgcgaa aattctgcat taatcactct ggcgcgcata
    22621 cccaagtgcg cgccatctca cgctttatcg tttcgctcct cgtatctgtt acatcctggc
    22681 gttacccttg tcgcaaagaa gcacacaaca aggagcaaca atggcactgc caattctgtt
    22741 agattgcgac ccaggtcatg atgacgctat cgcaatagtt ctcgccctcg cctcaccaga
    22801 gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag acaccagaaa aaaccttacg
    22861 caatgttctg cgtatgctga ccttgcttaa tcgcaccgat attccggtgg caggcggcgc
    22921 ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat gtgcacggcg aaagcggtct
    22981 cgacggcccg gcattaccgg aaccgacgtt cgcaccgcaa aactgtacgg cggtggagct
    23041 gatggcgaaa acgctgcgtg aaagtgcgga acctgtcacg attgtgtcta ccggaccgca
    23101 aactaacgtt gccttgctgc tcaatagcca tccggaactg catagcaaaa ttgcccgtat
    23161 cgtgattatg ggcggcgcaa tggggcttgg taactggacg ccagccgccg agtttaatat
    23221 ttatgttgac ccggaagcgg cagaaattgt cttccagtca gggatcccgg tggtgatggc
    23281 cggtctggat gttactcaca aagcacaaat tcacgttgag gacaccgagc gtttccgcgc
    23341 gattggcaac cctgtttcaa ccattgttgc cgaactgctg gatttcttcc tcgaatatca
    23401 taaagacgaa aaatggggct ttgtcggcgc accgctgcat gacccatgca ccatcgcctg
    23461 gctgttaaaa ccggagttgt ttaccactgt tgagcgctgg gttggcgtgg aaacacaagg
    23521 gaaatatacc cagggtatga cggttgttga ttattattat ctgaccggca ataaaccgaa
    23581 tgcctctgta atggtcgatg ttgatcgtca gggctttgtt gatttactgg ctgatcgtct
    23641 gaaattttac gcttaacaca cgtcattgat taagatcacc acgcgttacc tgggtggtct
    23701 taatctgttt atctcttttt ctttcaagat ataaatgcaa tggataatgc agaactcgcc
    23761 attggtatcg atctcggtac taccaatagt ttaattgccg tctggaaaga cggtgccgcg
    23821 caattaattc caaataagtt cggtgaatat ttaacaccat ccataattag catggatgaa
    23881 aataatcata ttttagtcgg aaaaccggct gtatcacggc gtacttcgca tccggataaa
    23941 acggcagcgt tatttaaacg tgcaatgggc agtaatacca actggcggtt aggcagcgac
    24001 acatttaacg cgccagaact gtcctctttg gtattacgct cattaaaaga agatgccgaa
    24061 gaatttctgc aacgtccgat taaagatgtg gtgatctctg ttccggctta tttcagcgat
    24121 gaacaacgca agcatacccg tttagcagcg gagttagccg gtttaaatgc ggtacgctta
    24181 attaatgaac ccacagcagc tgcgatggcg tatggcctgc atacccaaca aaatacccgt
    24241 tcgctggtgt ttgatctcgg tggcggcacg tttgacgtta cggtgcttga gtacgccacg
    24301 ccggtgattg aagttcacgc ctccgctggc gacaactttc ttggcggcga agattttact
    24361 catatgctgg tggatgaagt tttaaaacgc gccgcagtgg ctaaaaacat gctcaacgaa
    24421 agtgagctgg cagccctgta caccagcgtg gaagcggcaa aatgcggcaa tcaattaccg
    24481 ctgcaaatta gctggcagta tcaggaagaa acgcgtgagt gcgaatttta cgagaacgaa
    24541 ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag taccgattga acaggcgttg
    24601 cgcgatgcac gtctgaagcc aagtcaaatc gacagtctgg tgctggttgg cggcgcatca
    24661 caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc gtatcaaagt
    24721 tacgatccga gcaccattgt cgcgctgggc gcagcaatcc aggccgcctg ccgcttacgc
    24781 agtgaagata ttgaagaggt gatcctcact gatatttgcc cttactcgtt gggtgttgaa
    24841 gttaaccgcc agggcgtttc cggcattttc tcgccgatta ttgaacgaaa caccactgta
    24901 ccggtgtcgc gtgtagaaac ttactcgacc atgcacccgg aacaggattc aattacggtt
    24961 aacgtctatc agggagaaaa ccacaaagtt aaaaataaca ttctggtgga atccttcgat
    25021 gtgccgttga agaaaaccgg ggcttatcag tcgattgata ttcgctttag ttatgatatc
    25081 aacggcttgc ttgaagttga cgtgcttctg gaagacggca gcgttaagtc cagagtcatt
    25141 aatcacagtc ctgtaacatt gagcgcgcag cagattgaag agagtcggac gcggttatcc
    25201 gcattgaaaa tttatccgcg cgatatgctc atcaaccgta cctttaaagc caaactggaa
    25261 gagttgtggg cgcgggcgct gggtgacgag cgagaagaga tcggccgggt gatcaccgat
    25321 tttgatgcgg cgttgcagtc aaacgatatg gcccgcgtcg atgaagttcg gcgacgggcg
    25381 agcgattatt tggctattga gatcccttaa acacggctgc cggatgcggc ataaacgctg
    25441 catccggcat cggcgcaaaa aacacatcct cagctaaaca acatcgcgat ttttccgaga
    25501 gcataaccta tcaccagcac cgttaagctg attttgataa tactggtaat gctctgtggt
    25561 ttttcatcct catcattttt ttcttcgggg ttttcaggtg ttggctcatc gacatcttca
    25621 tcctccggct cgagtttcac tttcgtcaac agcatccggg tgataatggc ctctaatgga
    25681 tgagcatctc caccttccag ccttgtatta agacgggcca gcatctcttg tttcgggaac
    25741 gcttccagca caacctgttc cgctacccga tacaaatcca gtaacggtga atcttgttct
    25801 gtctcgctac cgactctcga tttccaccac gcccatgctt tgcgcttgaa gaataacgac
    25861 tcaataaacg attgacctaa cggcgataat tttccgctat gtaaaatttc cgcccataac
    25921 agccgcgtcc gggcgctctc accaggaaca tatttcaacg gttgaccttg cgcatcaacc
    25981 atgctctgcg cttcgataac catcactaac agcgcgaaat aatccgggca atgacgcttg
    26041 ctccactgca acaacaaatt atcggcttgt gtagatgggt aactctcccg gtatgcgcag
    26101 agatcggcaa gcaggctttc cccctcgcca cagtacaacc gttgcgcaca ctggtaataa
    26161 cgggcatctt cgctttctgg ttcttctgct tgccactgtt gggcaacagg aatgagttcc
    26221 gccataccta ttttcaccga gctgtaccag cgcaacagtt tgcgatgtaa cttgacatca
    26281 tcaggaatga accacggacc ttccattgcc ataaacgcag tgacatagtc gggatggtaa
    26341 cggcagcagc gttccagcgc gaagtaatac tcaacagtct ggttctgcaa cgcgacggga
    26401 tattccagaa ggctaaggaa atcgaaacaa tcaccagcct gaatggcctc aagaaaagcc
    26461 tccctttctt cctcatccat tccttcgctg tcattaaacg aatgccagtt caaacgctct
    26521 gccagcaaag aagcacaatt taatgaaaga tatcgcgatt ctatagcgat tgcacacagc
    26581 ggccagcgta attgatcgac atcctccatg ttccaggtat ttaattgctg aataaatttc
    26641 tgccaggcgg agggctgaaa acgatcactt tctgaatcca gtaatgtcct gaatgcacgt
    26701 agtatttcat gttcagcggc ggcatcttta ccttcattat ctgcttcctc gacaggattt
    26761 accgccagtc gcagcgcctc ttcataggcc tggcgtaatg ctttaaaccc ctgcggatcg
    26821 gtttcgggat gacacaacgg taagcgagca agataagcct ggcggataat gtctatttgc
    26881 gtcgtgcttt caatttcgag gatttgccaa catgttttca taggtcgctc acatccttaa
    26941 gcgttaccgg gcattcaggc tggacagtta atggctgcca gggaagcgtg gcatagaaac
    27001 gatcgcttcc cgctgccagc tcttcgaaca tccgactccc ggcgctatcg tatttgtaca
    27061 gggttagcgc ctcgcgtagc gaagcgttgt cttcgcactg agaagattgc cagtagagac
    27121 gccctaacga ataggcggaa aaatagtgca tccagctgtg atagtaatgg tgagctcgga
    27181 catagacgcg cgattgcagc cataaactct cgtcttccgt cagccagttg ttgagcacac
    27241 cgacccgtga cagaaacccc attcgaacat aatcccaggc tttgattccc ccttctccac
    27301 agcacatagc ggtttgggca acgaattggg tataaatcaa ccctctctcc gagccaccag
    27361 caattaatgc tttccactct tcaggggaag agcgatgcca tgtcagatat aagccagcta
    27421 aatcattggc atgaccatca tcagtcatct gataaatagt ctgaattaaa tcatcacgat
    27481 tatttattcc ccaggatgat gagagatcga attcttttat cccggtatac atttttggcg
    27541 agtcatgcgc gggtttgcaa ccctgattaa gtatttccat gggcgcagaa agcgcaaaaa
    27601 gaagatattg ttcttcctta ttcatttgtt aa
//
LOCUS       DAYPHN010000052        27419 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000052 DAYPHN010000000
VERSION     DAYPHN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.052"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            468..2057
                     /gene="purH"
                     /locus_tag="ACG0BW_002128"
     CDS             468..2057
                     /gene="purH"
                     /locus_tag="ACG0BW_002128"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFK7259083.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            2069..3358
                     /gene="purD"
                     /locus_tag="ACG0BW_002129"
     CDS             2069..3358
                     /gene="purD"
                     /locus_tag="ACG0BW_002129"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFK7259084.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     QEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLTDDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3355..4680)
                     /gene="zraR"
                     /locus_tag="ACG0BW_002130"
     CDS             complement(3355..4680)
                     /gene="zraR"
                     /locus_tag="ACG0BW_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFK7259085.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLADHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4677..6053)
                     /gene="zraS"
                     /locus_tag="ACG0BW_002131"
     CDS             complement(4677..6053)
                     /gene="zraS"
                     /locus_tag="ACG0BW_002131"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFK7259086.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDALPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKDPQG"
     gene            6291..6710
                     /gene="zraP"
                     /locus_tag="ACG0BW_002132"
     CDS             6291..6710
                     /gene="zraP"
                     /locus_tag="ACG0BW_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFK7259087.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAETGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            complement(6712..7407)
                     /gene="yjaH"
                     /locus_tag="ACG0BW_002133"
     CDS             complement(6712..7407)
                     /gene="yjaH"
                     /locus_tag="ACG0BW_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFK7259088.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7420..7692)
                     /gene="hupA"
                     /locus_tag="ACG0BW_002134"
     CDS             complement(7420..7692)
                     /gene="hupA"
                     /locus_tag="ACG0BW_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFK7259089.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            complement(7879..8469)
                     /gene="yjaG"
                     /locus_tag="ACG0BW_002135"
     CDS             complement(7879..8469)
                     /gene="yjaG"
                     /locus_tag="ACG0BW_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFK7259090.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            complement(8512..9183)
                     /gene="nfi"
                     /locus_tag="ACG0BW_002136"
     CDS             complement(8512..9183)
                     /gene="nfi"
                     /locus_tag="ACG0BW_002136"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFK7259091.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            complement(9193..10257)
                     /gene="hemE"
                     /locus_tag="ACG0BW_002137"
     CDS             complement(9193..10257)
                     /gene="hemE"
                     /locus_tag="ACG0BW_002137"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFK7259092.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            complement(10297..11070)
                     /gene="nudC"
                     /locus_tag="ACG0BW_002138"
     CDS             complement(10297..11070)
                     /gene="nudC"
                     /locus_tag="ACG0BW_002138"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFK7259093.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            11165..11641
                     /gene="rsd"
                     /locus_tag="ACG0BW_002139"
     CDS             11165..11641
                     /gene="rsd"
                     /locus_tag="ACG0BW_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFK7259094.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     regulatory      11693..11793
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11874..13769
                     /gene="thiC"
                     /locus_tag="ACG0BW_002140"
     CDS             11874..13769
                     /gene="thiC"
                     /locus_tag="ACG0BW_002140"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFK7259095.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEV"
     gene            13769..14404
                     /gene="thiE"
                     /locus_tag="ACG0BW_002141"
     CDS             13769..14404
                     /gene="thiE"
                     /locus_tag="ACG0BW_002141"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFK7259096.1"
                     /translation="MYQPDFPPVPFHLGLYPVVDSAQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAITLGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVMATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            14397..15152
                     /gene="thiF"
                     /locus_tag="ACG0BW_002142"
     CDS             14397..15152
                     /gene="thiF"
                     /locus_tag="ACG0BW_002142"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFK7259097.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINTACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDTQDPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRATGCPVCGGSNADPV"
     gene            15136..15336
                     /gene="thiS"
                     /locus_tag="ACG0BW_002143"
     CDS             15136..15336
                     /gene="thiS"
                     /locus_tag="ACG0BW_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFK7259098.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            15338..16108
                     /gene="thiG"
                     /locus_tag="ACG0BW_002144"
     CDS             15338..16108
                     /gene="thiG"
                     /locus_tag="ACG0BW_002144"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFK7259099.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAIADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            16105..17238
                     /gene="thiH"
                     /locus_tag="ACG0BW_002145"
     CDS             16105..17238
                     /gene="thiH"
                     /locus_tag="ACG0BW_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFK7259100.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(17648..18187)
                     /gene="yjaZ"
                     /locus_tag="ACG0BW_002146"
     CDS             complement(17648..18187)
                     /gene="yjaZ"
                     /locus_tag="ACG0BW_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFK7259101.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARIHLKRIILALPES
                     NVIHAGYGSYAVIEVDLNGRDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(18401..22624)
                     /gene="rpoC"
                     /locus_tag="ACG0BW_002147"
     CDS             complement(18401..22624)
                     /gene="rpoC"
                     /locus_tag="ACG0BW_002147"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFK7259102.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(22701..26729)
                     /gene="rpoB"
                     /locus_tag="ACG0BW_002148"
     CDS             complement(22701..26729)
                     /gene="rpoB"
                     /locus_tag="ACG0BW_002148"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFK7259103.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(27049..27414)
                     /gene="rplL"
                     /locus_tag="ACG0BW_002149"
     CDS             complement(27049..27414)
                     /gene="rplL"
                     /locus_tag="ACG0BW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFK7259104.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
BASE COUNT         6304 a         7525 c         7181 g         6409 t
ORIGIN      
        1 gacgcggctt tcagctcact gtcgcgaggt ggcgtatatt acgctttcct ctttcagagt
       61 caaccctgaa tttcaggatt tttctcttca accgaaccgg ctgtttgtgt gaagtgattc
      121 acatccgccg tgtcgatgga ggcgcattat agggagttct ccgcaggccg caatagaaaa
      181 attgcagaaa aatgactgac tgctgcattc cccagcaaaa ccccgcttta taccttttta
      241 cgcacagagt tatccacaat catcaatgta atttctgtat ttttcccacg gtaaacactg
      301 tcaaaattgt gatcaccatt gaaagagaaa aattcgcgag cgttgcgcaa acgttttcgt
      361 tacaatgcgg gcgaaaaata aggatgcccc gttaggggcg ttagctgagt ttttcgcgaa
      421 aaattcagct aacgctctct gtaatagtca aatccagggg atttaccatg caacaacgtc
      481 gtccagtccg ccgcgctctg ctcagtgttt ctgacaaagc cggtatcgtc gaattcgccc
      541 aggcactttc cgcacgcggt gtggagctgc tgtctacagg gggcactgcc cgtctgttag
      601 cagaaaaagg tctgccggta accgaagttt ccgattacac cggtttcccg gagatgatgg
      661 atggacgcgt gaagaccctg catccgaaag tacatggtgg cattctgggc cgtcgcggcc
      721 aggacgatgc cattatggaa gaacatcaga tccagcctat cgatatggtg gttgttaacc
      781 tgtatccgtt cgcccagacc gttgcccgtg aaggctgctc gctggaagat gcggttgaga
      841 acatcgatat cggcggccca acgatggtgc gctccgccgc caagaaccat aaagatgtcg
      901 ccatcgtggt gaagagcagc gactacgacg ccattattaa agagatggat gccaacgaag
      961 gatcgctgac gcttgcaacc cgtttcgacc tcgccatcaa agccttcgaa cacactgccg
     1021 cctacgacag catgattgcc aactacttcg gcagcatggt tccggcttac cacggtgaaa
     1081 gcaaagaagc cgccggtcgc ttcccgcgca cgctgaacct gaacttcatt aagaagcagg
     1141 atatgcgtta cggcgagaac agccaccagc aagctgcctt ctatatagaa gagaatgtga
     1201 aagaagcctc cgttgctacc gcaacccagg ttcagggtaa agccctctct tataacaaca
     1261 tcgccgatac cgatgcggcg ctggagtgcg tgaaagagtt cgccgagccg gcatgtgtga
     1321 ttgtgaagca cgccaaccct tgcggcgtgg ctatcggcaa ttccattctt gatgcttacg
     1381 atcgcgcgta caaaaccgac ccgacctccg cattcggcgg cattatcgcc tttaaccgcg
     1441 agctggatgc ggaaaccgcg caggccatca tttctcgtca gtttgttgaa gtgattattg
     1501 cgccgtccgc cagcgaagaa gccctgaaaa tcaccgccgc caaacagaac gtacgcgttc
     1561 tgacctgcgg tcagtggggc gagcgtgttc cgggtcttga tttcaaacgc gtgaacggcg
     1621 gtctgctggt tcaggatcgt gacctgggga tggtcggtgc ggaagaactg cgcgtcgtca
     1681 ccaaacgtca gccgactgaa caagagctgc gtgatgcgct gttctgctgg aaggtggcga
     1741 agttcgtgaa atccaacgcc atcgtctatg ccaaaaacaa tatgaccatc ggcattggcg
     1801 cgggccagat gagccgcgtg tactccgcaa aaatcgccgg tattaaagcg gccgatgaag
     1861 gtctggaagt caaaggttcc tcgatggctt ctgacgcgtt cttcccgttc cgcgacggta
     1921 ttgatgccgc cgccgctgcg ggcgtgacct gcgtaatcca gcctggcggt tctatccgtg
     1981 atgacgaagt gattgccgcc gccgatgaac acggtattgc aatgctcttc accgacatgc
     2041 gccacttccg ccattaatgg agcaatagat gaaagtatta gtgattggta acggcgggcg
     2101 cgagcacgcg ctggcctgga aagcggccca gtcgccgctg gttgaaactg tttttgttgc
     2161 tccgggtaat gcaggcactg cgctggaacc cacactgcaa aacgtcgcta tcggcgtaac
     2221 cgatatcccg gcgctgctgg atttcgcgca aaacgaaaag atcgatctaa ccatcgtcgg
     2281 cccggaagcg ccgctggtga aaggcgtggt ggataccttc cgtgccgccg ggctgaaaat
     2341 cttcggccca accgcaggtg ccgcgcagct ggaaggctcg aaagcgttta ccaaggattt
     2401 cctggcccgc catcagatcc ctacggcgga ataccagaac ttcaccgagg tagaacctgc
     2461 gctggcgtat ctgcgtgaga aaggcgcgcc aatcgtcatt aaagctgacg gtctggctgc
     2521 cgggaaaggc gttatcgtgg cgatgacgca ggaagaagcg gaagcggctg ttcacgatat
     2581 gctggcaggc aacgcttttg gcgacgcggg gcatcgcatc gttatcgaag agttcctcga
     2641 cggcgaagaa gcgagcttta tcgtgatggt ggacggtgag catgtgctgc cgatggccac
     2701 cagccaggat cacaaacgcg taggcgataa agataccggg ccaaacaccg gcgggatggg
     2761 cgcttactcc cctgcgccag tagtgaccga tgaggttcat cagcgcacga tggaacgaat
     2821 catctggcca accgtgaaag gcatggcggc agaaggcaac acctacaccg gttttctcta
     2881 cgcgggcctg atgatcgaca aacagggcaa tccgaaggtt atcgaattta actgccgctt
     2941 tggcgatcca gaaacccagc cgattatgct gcgcatgaag tccgatctgg ttgagctttg
     3001 cctggcggcc tgtgaaggca agctggacga gaaaacgtcc gagtgggatg aacgtgcttc
     3061 tctcggcgtg gtaatggctg cgggcgggta tccgggtgat tatcgcaccg gtgatgtgat
     3121 ccacggcctg ccgctggaag aagtggcagg cggcaaagtg ttccacgcgg gcacaaaact
     3181 gacggatgac gagcaggtgg taaccagcgg cgggcgcgta ctgtgcgtca ccgcgctggg
     3241 tcataccgtg gcagaagcgc agaaacgcgc ttatgcctta atgaccgata tccattggga
     3301 cgactgcttc tgccggaaag atatcggctg gcgcgctatc gaacgcgagc agaactaacg
     3361 cgacagtttt gccaacagcg ttttgcgcgt gatccctaac tgacgggcgg cttcggtttt
     3421 gttgccgccc gttttctcca gtgccgcaag aatcacctct ttttcgactt ccaccagcgg
     3481 ctgaatatcc tgactttgta ccagcgggat cggcgtactc gcaatcgcca gcggcagctc
     3541 gcgttcggaa atatattccc cggtcagcag caccaccgcc cgttccaccg cgttttccag
     3601 ttcacgaata tttcccggcc agtcgtagtg aatcagcaga tccatcgcct ggggcgtaaa
     3661 accttttacc gccttgcgat tacgctcggc aaagcgctga agaaaatggt cagccagcag
     3721 aggaatatct tcccgccgct gacgcagcga cggtacttca atcgccacca cgttcaggcg
     3781 atagtagaga tcctggcgaa aacgcccggc attcacctct gcggcaagat cgcgatgggt
     3841 cgcggcaatc agccggacat caaccgagat ggtctggttg ctgccgacac gctgaacttc
     3901 gcgctcctga atcgcacgca gcagacgaac ctgcatcatc ggcgagatat cgccaatttc
     3961 atcgagaaac agcgtgccgc cgtccgcctc aacaaagcgc ccttcccgcc gtttgtcggc
     4021 tccggtaaac gccccttttt cgtgaccgaa caattcagat tccagcaagg attcgttgag
     4081 tgccgcacag ttgagcgtta ccagtggttt ttcactccgt gcgctactgg cgtgaatcgc
     4141 cctggcgacc agctctttac cggtgccgga atcgccgtgg atcagcaccg tggcttccga
     4201 tggcgcgacg agggcgattt cactgagcag gtgttgcatc gccgggcttt taccgaccat
     4261 accgaactgg ctggcagaca ccgcaggcgt ttcagcgtca acactgtgcg tatgtgcgag
     4321 cgccttttcc agcgtcgcct gcaggttatc gaaatccagc ggcttgatga gataatccag
     4381 cgccccggtt ttcaacgcct ctaccgccgt ctcgacgctg gagtacgcgg tcataatcag
     4441 taccggaatt gccggattta acgctttgat ctctttgagc gtggcgatgc cgtccatctc
     4501 tgccattcgc acatcgcaaa gcacaagatc aaaaacctgt tcccgcacct gctccagcgc
     4561 ctgtcgcccg ctgttcgcca acgcgacgtt atagccccag ccgcgcagca aagcctgcaa
     4621 aatagtgcag tggctaatgt catcatccac caccagaata tcgatattat cgtgcgtcat
     4681 ccttgtgggt ccttacgcgt aatagtgacc ggaagccaga gggtgaacgt tgcgcctttt
     4741 ccctcctggc ttgcgacctg aattgtacca ccgtgttgtt caacaatatt atgcacgacc
     4801 gccagcccca gtccggtgcc ttcggcttta gtggtgaagt acggagtgaa gatggcatca
     4861 agctgatctg ccgcaattcc cttaccgctg tcggtaacgc tgattttcac gcccgcgccg
     4921 ctttcgctgg ccgtcacgct aatcacgcca tgctgaccaa tcgcctgaat agcattgaga
     4981 tagagattca acaggacctg agtcagcctg tccgggtcgg cctgaatttc cggcaacgcg
     5041 tcgttggcgg taaagcgtaa ctggatctcc cggctgtttg catcctggct taccagctgt
     5101 aatgagtggt taatcagcgt gttgagatcc accgcctgca aagccagatg cgttggcttg
     5161 accagttcca gcaactcgct taccacgcgg tttaaacggt cggcttcttt cgccatcacc
     5221 tgcgccagtt gatgcgcttc tccccctgct ggcgcacgtt cggcaaagta tttcgccagc
     5281 cctttaatcg aggaaagtgg gttacggatt tcgtgggcga cgcctgccgc cagatgcccc
     5341 agcgccacca gcttctcttt gcgcttcatc tcatcctgta gaagctggcg cgagcgcaga
     5401 tagcggcgat accagaagaa tgacaacacg cttgccagca agaccgtcgc cagggcgaag
     5461 aggataatca gggtgtttct ctgctcacga tcctcagcac tcacaatgtt actggcgtcg
     5521 aaagcgataa aaattgcttg tggtgtcgcg gcatactgct gcatatggcg catccggtgc
     5581 attccagcag caaacatcgg ttgaaactgg cgataaattt ccagaacagg ctcgccgtct
     5641 gcgctatcga tccgccgcca cgcttcttca tctcccggat gtaactgctg catttcctgc
     5701 ggggaataaa gctgttttcc caccatgccg gagttgctat gcatcacgat tgttccttgt
     5761 tcatccgtga ccgcaaacca acgtacacca ggttgcccgg ccatttcttc cagtaatgcc
     5821 tgctgctgcg catgatgcat gcgcatcccc atgccgacgc gcgagccgga ttcgagagca
     5881 cggataagta cactgccttt ttccagcagc gtttgtctgg cggcggcagt ctcgcgccca
     5941 taatcgcgaa tcactgtcac cgcaaacagc cccaccagcc caacaatgac cacggggagg
     6001 atcgcgctta accatttagc taaggagtct ttagaacgtt gcataaaacg catcttcttc
     6061 tttgcctgct catccctttc tgacagcaga aatcatgcca tcttttatca gcgcttaccc
     6121 tgcgctgtaa cacaaaggct taagtttcaa tgagtaaaaa tgactcgcta cccgcagcag
     6181 gcgagtcatt tttactcgcc tattacgtca gattgcccgt catatcagcg tttcgtcgtt
     6241 ggcacggaag atgcaatacc cgaagtaaga caaccactgg aggattaacc atgaaacgga
     6301 acacgaaaat tgccctggta atgatggcgc tttcagcaat ggcgatggga tcgacatctg
     6361 catttgctca cggcggacac ggtatgtggc agcaaaatgc cgcgcctttg accagcgaac
     6421 aacagacagc gtggcagaaa atccataatg acttttacgc tcaaagcagc gcactgcaac
     6481 agcaactggt gacgaagcgt tatgaataca atgccctgtt agccgcgaac ccaccggata
     6541 gcagcaaaat taacgcggtc gccaaagaga tggagaattt gcgtcagtcg ttagatgagt
     6601 tacgggtgaa acgagatatt gcgatggctg aaacgggtat tccgcgcggt accggaatgg
     6661 gttacggcgg ctgcggtggt ggcggtcata tgggtatggg ccactggtaa atcagaacgt
     6721 tttctcgttg ggttgccagc gacagaaatc agagtttgcc actaacagta attgcgaacc
     6781 ttcgggcgct tccagccatg ccacgctgac atcaacagaa gagcggctct ggcggcgctc
     6841 gatatgcgct atcgcctgcg aatctaaatc aggtttaatg gtttgccctt cgcaggtggt
     6901 cacagtctgc tccatcgcat gccagcgccc ctggcgcagc acgactctcc cctgacgtaa
     6961 ggcatcgcta atctggcgga tctgatcagc acgataacgg tataaatcga tttggtcgtt
     7021 agaaagctgc tgcttttccc catcggattc acgttgcata aagctcagat cgccgcgatc
     7081 gtcaaaacgc gcgcgaatgt tcaccggcgg tttgctgtaa acattgatat tgatgagcgt
     7141 aaggttatcg ccctgccagc gatactctcg cgtcgtggta tcgccactgc gccacgggct
     7201 aaacacggca agcagatgca cattatcgcc gctgtctttt cgccagatac gtaccgcgcc
     7261 ctggtcttca gcaaatccac tggcggtaaa gggaggaagt gaagagttgt ggctacaggc
     7321 agtgagaaac agagcgccag ccagcatcaa tgatcgacgc cagaaagaca aaaggggtga
     7381 aaccacccct tcgttaaaac tgttcactgc cacgcaatct tacttaactg cgtctttcag
     7441 tgccttgcca gaaacaaatg ccggtacgtt agctgcggca attttgattt ctttaccggt
     7501 ctgcgggttg cggccagtac gctcagcgcg gtggttcact ttgaaggtac cgaaaccaac
     7561 cagttgtaca gcatcgcctt ctttcagaga ctcagtaatt gcggccagag tggactccag
     7621 agcagcttta gcctgggttt tggacagttc tgctttctct gcaattacat caatcagttg
     7681 agtcttgttc ataagttatc cttacaatgt gtttatcgct tgctaagcat cgagtgcgac
     7741 ggaaatgcca gaatagcact ctcctgcata cacgcaccga tagccacttt ttttcgcccc
     7801 ccaaatgtag accagacggg gggcgaaagg gaagactcca ggtacgacaa atcaggcgtt
     7861 aaatcacgtt ttctggtctt attgctgaaa aattatacca atattgctct cacccgcctc
     7921 acgcaggtct gccctaaggc cttttatcag ctcgatatcg cgttcttcgc actcagctaa
     7981 aagtcggaat atttcccact gaatgtccca ttcttgctct acagctgggt tttctttgag
     8041 ctcttcatcg ctcatttcgc gaccagcctg agtcatttcc agcatcgcaa cggtcgtgat
     8101 ggaggtctta ctcacttcca ccgcgtgttc gagcgtttca ccactcaaac gcgaatggac
     8161 cagttcactt aacgccacgc atgcatcgat tgccggataa acgccgtaca gatcgaaatc
     8221 gtcggctgaa ggaatcgctt cttcaaattt ctccagttgg ctgtcgaaat ttacttttgc
     8281 gtctttgacg gtcagcgttt cccagatgag atcgagaatg cggcggtaaa tctgcccatc
     8341 accaaaacca gtttgctgac agaatacggc gtaattgggg tacatgcgtt cgcataagca
     8401 ggccataaaa gtgacgtgct gccagctttc caggcgctcc agacgcagat gaattgggtt
     8461 ttgtaacatg ttgagttctc aaatacggaa attatccgca gtttacctga attagggctg
     8521 atttgctgta tagcgcacga acgccggacg ttccgaggcc accgcgtccg cccagcgcgt
     8581 tggctccggc agacgatagc ctttcatgca gcgttgtacc cacgccagcg cgctgtccac
     8641 gctgacccga tgaccggtag cgataaacaa cgggttacag cgcgctttgc tgcgccagac
     8701 ccatgccagc tgctcgcctt tatccatcag tggggccagc gcgcccggtt cgctggagag
     8761 cggttcgaat ttaccgcaga gccgtttttt cgctacgcca atggtcggca catccaccag
     8821 caaaccaaaa tggctggcga cgccaagacg gcgaggatgc gagatcccgt gaccatcgac
     8881 aaacactaaa tccggctttt gcgacagcat ctcccacgct gccagcagcg caggatattc
     8941 gcggaacgag agaaaacctg gaatgtaagg catggtggtg gcgatgcggg ccactttata
     9001 ctccaccagc tcaagcgagg ggtatttcag cagcaccatc gccgctcgcg tcacttctcc
     9061 gccctgctca aatccgacat cggctccggc gatcagatcc ggcggatctt tatcgagtcg
     9121 atcctcgcgg atcacagaag aagccagttc aatttgttga gcgcgtaatg acgcgagatc
     9181 cataatcgct ccttagcggt gatattgttc agacaagcga tgcactgcct ccacaaatac
     9241 gccagcatgt tctggcggca catcctgatg aatgccgtga ccaaggttaa agacatgacc
     9301 ttcgccgtga ccgaaacctg caagtatagt cgctacttct tcttcaatgc gggctggcgg
     9361 cgcgtacagc atcgacggat ccatattacc ctgcaacgcg actttattgc ccacgcggcg
     9421 gcgtgcatcg gcgatatccg ttgtccagtc gaggcccagc gcatcgcaac cggtttctgc
     9481 catggcttcc agccactgtc cgccgccttt ggtaaacagc gtgaccggta cgcggcgacc
     9541 gtcgttttca cgcagtaaac catcaacaat tttatgcatg taataaagcg agaactgttg
     9601 ataatcgcgc ccggtaagca cgccgcccca ggtgtcgaaa atcatcactg cctgagcacc
     9661 ggctttaatc tgcgcattca gatacaaagt gacgcttttc gccagtttat cgagtagcgc
     9721 gtgcagcgcc tgtggatcgg catacatcat ttttttgatc acggtgaacg ctttgctgct
     9781 gccgccttcc accatatagg tcgccagcgt ccacgggctg ccggaaaaac caatcagcgg
     9841 cacttcgcct ttcagttcgc gacgaatggt acgcaccgcg ttcatcacgt aacccagctc
     9901 atcttccggg tccggaattg gcagtttatc gacgtcggct ttgcaggtga ctggcgaggt
     9961 aaaacgcgga ccttctccgg cttcaaaata gagccctaac cccatcgcgt ccggcacggt
    10021 gaggatatcg gaaaagagga tcgccgcatc cagcggatag cgacgcagcg gttgcaaagt
    10081 cacttcgcac gccagctcgg cgtttttgca cagcgacata aaatcgcccg cctgggcgcg
    10141 cgtggcttta tattccggta gatagcgacc cgcctggcgc atcatccata ctggagtgac
    10201 atcaacgggc tggcgcagca gcgcccgcag ataacgatcg tttttaagtt cggtcatttt
    10261 ggctgttcct tagtgcgtca ggcggtcagt gtatcatcac tcatactctg cccgacacat
    10321 cgccaccgta tcttctatca gacggcgcgc tacggtgccg ggcggcggga gtaacggcaa
    10381 atcgtcatag cgataccagt tcgcctcgag caattctttc gggtcgatca cgatgtcgcc
    10441 gctgtcatat tccgccataa acgcggtcat taaagactga ggaaacggcc acggctggga
    10501 agtcacataa cgcaagtttt taactttaat tccgctctct tccatcactt cccgcgcgac
    10561 cgcctgctcg agggtttcgc ccacttcgac gaatccggca agtaccgtat ggacaccgtt
    10621 acgatggcgg gtatgctggg cgaggaggat cgaatcatcg cgacggatgg caacaataat
    10681 gcagggggcg atttgcggat agtaacgctc acggcaatgg ctgcacagca tcgcccattc
    10741 ggttttgctt ggatacattt catgcccgca gtaaccacag tatttatgcg atcggtaaaa
    10801 ctccgccagt tgcacgccac gtccggccag ttgaaacagc ccaacatcga gatcaatgac
    10861 ctgacgtacc gaccccatat cgtgacgccg ctgctgttgt attaaccaaa caggttcccc
    10921 ctgccattcg ccgatttgta gtgcgcgctg acccacaaga tcgaaatttg ccgcttcgcc
    10981 atatggcaat tctcccttcg gcaaccataa tttttgttca tggctgacga cccaccagcc
    11041 gtgatctaat ttttcaatta tacgatccat agctcttgca ctacctttgc atcactggca
    11101 tgtttaacat ggtttttaca tttctcactg agcagttttt gaatacaaac ttgcggagtc
    11161 aatcatgctt aaccagctcg ataacctgac ggagcgcgtc agaggaagta acaaactggt
    11221 tgatcgctgg ctacatgtac gtaagcatct gctcgtggct tactacaatc tggttggcat
    11281 taagcctggc aaagaatcgt acatgaggct aaacgaaaaa gcccttgatg atttttgtca
    11341 gagcctggtc gattacttgt ctgccggaca tttcagtatt tatgagcgca ttcttcataa
    11401 gctggaaggc aacgggcaac tcgcgcgcgc cgcgaagatt tggccgcaac tcgaagccaa
    11461 tacccaacag attatggatt actacgattc cagcctggaa accgccatcg atcatgataa
    11521 ttaccttgag tttcaacagg ttttatctga catcggcgag gcgctggaag cgcgctttgt
    11581 gctggaagat aagctgattc tgctggtgct tgacgccgcc cgcgtcaaac atcctgcttg
    11641 agttctgtgc tgttaacgcg taatttacat tcaacgcccc gtttgtgggg ctaatttctt
    11701 gtcggagtgc cttaactggc tgagaccgtt tattcgggat ccgcggaacc tgatcaggct
    11761 aatacctgcg aagggaacaa gagttaatct gctatcgcat cgcctttgcg gcgatcgtct
    11821 cttgcttcat ccgtcgtctg acaagccacg tccttaactt tttggaatga gctatgtctg
    11881 caacaaaact gacccgccgc gaacaacgcg cccaggccca acattttatc gacaccctgg
    11941 aaggcaccgc ctttcccaac tcaaaacgca tttatatcac tggcacacaa cccggcgtgc
    12001 gcgtgccgat gcgtgagatc cagcttagcc cgacgctaat tggcggtagc aaagaacagc
    12061 cgcagtttga agaaaacgaa gcgattccgg tctacgacac ctccggcccg tatggtgatc
    12121 cgcagatcgc cattaacgtg cagcaaggac tggcaaaact acgccagccg tggatcgatg
    12181 cgcgcggcga taccgaagaa cttaccgtgc gcagttccga ttacactaaa gcgcgactgg
    12241 cagatgatgg cctcgacgag ctacgtttta gcggcttatt aacgccaaaa cgcgccaaag
    12301 caggacgccg cgtcacccaa ctgcactacg cccgccaggg catcatcaca ccggaaatgg
    12361 aattcatcgc catccgcgag aatatgggcc gcgagcgcat acgtagcgaa gttttacgcc
    12421 accagcatcc gggaatgagc tttggcgcac gtctgccgga aaatatcact gcggaatttg
    12481 tccgtgatga agttgctgcc ggacgcgcga ttatcccggc caacattaat catccggaat
    12541 cggagccgat gattattggt cgcaatttcc tggtaaaagt taacgccaat atcggcaact
    12601 cggcggtcac ctcttccatc gaagaagaag tggaaaagct ggtatggtcc acgcgctggg
    12661 gagcggatac ggtgatggat ctctccaccg gtcgctatat tcacgaaacc cgcgagtgga
    12721 ttttgcgtaa cagcccagtg ccgattggta cagtgccgat ctaccaggcg ctggagaagg
    12781 ttaacgggat cgccgaagat cttacctggg aagcgttccg cgacacgctg ctggaacagg
    12841 ccgagcaagg tgtggattac ttcactatcc atgcgggcgt actgctgcgc tatgtgccga
    12901 tgaccgcgaa acgcctgacc ggtatcgtct ctcgcggcgg ttcgattatg gcgaaatggt
    12961 gcctctccca tcatcaggaa aatttcctct atcaacactt ccgcgaaatt tgtgaaatct
    13021 gtgccgctta tgacgtttcg ctgtcgctgg gcgacggtct gcgccccggt tctgttcagg
    13081 acgccaacga tgaagcgcag tttgccgagc tgcatacgct gggtgaactg accaaaatcg
    13141 cctgggaata tgacgtacag gtgatgattg aaggcccagg ccacgtgccg atgcagatga
    13201 tccgccgcaa tatgaccgag gagttagagc actgccacga agcgccgttt tacactctgg
    13261 ggccgctaac taccgatatt gcgccgggct atgaccactt cacgtcgggg attggtgcgg
    13321 cgatgattgg ctggtttggc tgcgcgatgc tctgttacgt aacgccaaaa gagcatctgg
    13381 gtctgcctaa taaagaagat gttaagcagg ggcttatcac ctataagatt gccgcccacg
    13441 ccgctgacct ggcgaaaggg catccgggcg cgcaaattcg cgataacgcc atgtcgaaag
    13501 cccgcttcga atttcgctgg gaagaccagt ttaatctggc cctcgacccg tttaccgccc
    13561 gcgcttatca cgatgaaacc ctgccgcaag aatccggcaa agtcgcccat ttttgctcca
    13621 tgtgtgggcc gaaattctgc tcgatgaaaa tcagccagga agtgcgtgat tacgccgccg
    13681 cgcaaaccat tgaagtggga atggcggata tgtcggagaa cttccgcgcc agaggcggag
    13741 aaatctacct gcgtaaggag gaagtgtgat gtatcagcct gattttcctc ctgtaccctt
    13801 tcatttagga ctgtacccgg tggtggacag cgcacagtgg atcgaacgtc tgttggatgc
    13861 aggcgtgcgt actctccagc tacgcatcaa agatcggcgc gatgaagagg tggaagccga
    13921 tgtcgtggcg gcaattacgc tgggccgccg ctataacgcg cgattgttta tcaacgatta
    13981 ctggcggctg gcgatcaagc atcaggcgta tggcgtccat ctggggcagg aagatttgca
    14041 agccaccgat ctcagcgcca tccgcgcggc aggtctgcga ctgggcgttt cgacccatga
    14101 cgatatggag atcgacgtcg cgctggcagc acgcccctct tatatcgcgc tgggacatgt
    14161 gttcccgacg caaaccaaac agatgccttc tgcaccgcag gggctggaac agctggcacg
    14221 gcatgttgag cgcctggcgg attatcccac cgtggcgatt ggcggtatca gtctggcgcg
    14281 cgcgcctgcg gtaatggcga cgggtgtcgg cagtatcgcc gttgtcagcg ccattactca
    14341 agccgcagac tggcgtttgg caacggcaca gttgctggaa attgcaggag ttggcgatga
    14401 atgaccgtga ctttatgcgt tatagccgcc aaatcctgct cgacgatatc gctcttgacg
    14461 ggcagcaaaa actgctcgac agccaggtgc tgattattgg tcttggcggg ctgggtacac
    14521 ctgctgcgct atacctggcg ggcgctggcg tcgggacgct ggtactggca gatgacgacg
    14581 atgtgcattt aagcaatctg caacgacaaa tcctctttac cactgaagat atcgatcgcc
    14641 cgaaatcgca ggtcagccat cagcgactga cacagttgaa tcccgatatt caactgacag
    14701 cattacaaca acggttaacg ggtgaggcgt taaaagatgc ggtagcacgg gccgatgtgg
    14761 tgctcgactg taccgacaat atggcgactc gtcaggagat taataccgcc tgcgtggcac
    14821 tcaacacgcc gcttatcacc gccagcgcgg tcggatttgg cggtcagttg atggtactga
    14881 cgccgccctg ggagcagggg tgttaccgct gcctgtggcc agatacccag gatccagaac
    14941 gcaactgccg cacggcgggc gtggttggcc cggtggtcgg ggttatgggc actttgcagg
    15001 cactggaagc cattaagttg ttaagcggta tagagacgcc tgcgggagaa ctccgactgt
    15061 tcgacggtaa gtctagccag tggcgcagcc tggcgctgcg ccgcgctaca ggttgcccgg
    15121 tatgcggagg aagcaatgca gatcctgttt aacgatcaac cgatgcagtg cgccgccggg
    15181 caaacggttc acgaactact ggagcaactc gaccaacgac aagcgggcgc ggctctggcg
    15241 attaatcagc aaatcgtccc gcgtgagcag tgggcgcaac atatcgtgca ggatggcgac
    15301 cagatcctgc tttttcaggt tattgcaggg ggttgagatg ttacgtattg cggacaaaac
    15361 gtttgattca catctgttta ccggcaccgg gaaattcgcc tcatcacaac tgatggtgga
    15421 gtctattcgc gcttccggca gccaactggt gacgctggcg atgaaacgtg tcgacttgcg
    15481 ccagcacaac gacgctatcc tcgaaccgct tatcgcggcg ggtgtgaccc tgctaccgaa
    15541 tacatccggg gcgaaaacag cggaagaagc cattttcgcc gcccatctgg cacgtgaagc
    15601 attaggcaca aactggctaa aattagagat tcaccctgac gcccgctggc tgttgcccga
    15661 tcccatcgaa accctgaaag ccgccgaaac gctggtacaa cagggatttg tcgtgctgcc
    15721 ttactgcggg gccgatccgg tattgtgtaa acgtctggaa gaagtcggct gtgcagcggt
    15781 gatgccgctc ggcgcgccga ttggctcgaa tcagggactg gaaacccgtg ccatgctgga
    15841 gattattatc cagcaagcca cggttccggt ggttgtcgac gctggcatcg gcgttcccag
    15901 ccatgccgcg caggcgctgg aaatgggggc cgacgcggtg ttagtgaata cggcgattgc
    15961 catcgcggac gatcccgtca acatggcgaa ggcatttcgt ctggcagtag aagcaggcct
    16021 gctggcacgt cagtccggac cgggcagccg cagtcatttt gctcatgcca ccagcccgct
    16081 gaccggattt ctggaggcat cggcatgaaa accttcagcg atcgctggcg acaactggac
    16141 tgggacgaca tccgcctgcg tatcaacagc aaaacggctg ctgacgtaga gcgggcgcta
    16201 aatgcctcgc aactcacccg cgacgatatg atggcgctgt tatcgccagc cgccagtggc
    16261 tatctggaac cactggccca acgggcgcag cgtctgaccc gtcaacgatt tggcaacact
    16321 gttagcttct acgtcccgct ttatctttcc aatctttgcg ctaacgactg cacgtactgc
    16381 ggattctcta tgagcaaccg catcaagcgc aaaacgctgg atgaagcgga tattgccagg
    16441 gaaagcgccg ctatacggga gatgggcttt gaacatctgc ttttagtcac tggtgaacat
    16501 caggcgaaag tggggatgga ttactttcgt cgtcatctcc ctgccctgcg tgaacagttc
    16561 tcttcactac agatggaagt acagccgctg gcggagacgg aatacgccga gttaaagcaa
    16621 cttggtctgg atggcgtgat ggtttatcag gagacatatc acgaggcgac ttatgcccgc
    16681 caccatctga aaggcaaaaa acaggacttc ttctggcggc tggaaacgcc ggatcggctg
    16741 ggccgtgcgg ggattgataa gataggcctc ggcgcgctaa ttggcctttc cgacagctgg
    16801 cgcgttgact gctatatggt tgcagaacat ttgctatggc tgcaacagca ttactggcaa
    16861 agccgctact ctgtctcctt cccacgcctg cgtccatgta ctggcggcat tgagcctgcg
    16921 tcgattatgg atgaacgcca gttagtgcaa accatctgcg ccttccggct gcttgcaccg
    16981 gagattgaac tgtcactctc cacgcgggaa tcaccgtggt ttcgcgatcg cgtgattccg
    17041 ctggcaatta ataacgtcag cgctttctcg aaaacgcagc caggtggcta tgccgacaat
    17101 caccccgagc tggaacagtt ctcaccacac gacgatcgca gaccggaagc ggttgctgcc
    17161 gcgttaaccg ctcagggttt gcagccggta tggaaagact gggacagcta tctgggacgc
    17221 gcctcgcaaa ggccatgaga cggaatgtaa gaacgtaaat ttattccgag ccgctacgga
    17281 agatgaaaaa cgcaaggttg ttgaaagcgt tgtgtttttt tatggtagtg ccgtcagcag
    17341 gaatgttgtc cagggcgaaa agcttcttcc tcgtttcgcc ctgcctttct taaaaattcc
    17401 gtaatgcaaa gtctgaagcc atcgcctgtt gagcaatgaa agagatctga ttgtaagaga
    17461 gtaaatactc aactatgata gagacgaaaa taagaacaca tgttctcatc ttccaggatg
    17521 cagcagactg aagaaattca gacatcccgc aacctgcgat tatcgcaagg tcaaggcaaa
    17581 gtccggtaat ggcgttctga ataccagaga taattctctg gcgaaaccca ccttaaggtg
    17641 ggttttgtta ttttgagggc tgaggaagct gcttatttct caataagttg taggcaatgg
    17701 tgaaaaattt cactgattta cctttctgtt tttcagaaat gggataagcg ctagttacat
    17761 gcaaacgaag ttttttcttt tccctgaaag ccctgaacgc aacaaagtaa aatgccttgt
    17821 ctcttccgtt taaatccact tcaatcacgg catagctacc atacccagca tgaataacgt
    17881 tgctttctgg cagcgccaaa atgattcttt ttaagtgtat gcgcgctaag ttataacggt
    17941 gctggcagaa gggacgagat tctttaggcg cgtggtacat taacgattgt ttttcttcgt
    18001 tagtctgatg ttcataatct tttgtgaagc agtgaagaga gtaggtaacc cagaagcgat
    18061 actctaaagg ctctttgccc tcatgatgct gaacgtagac agcctgatga gcatcaagaa
    18121 atgataaatc acgaatatca ccatccggat gtccaaaagg tcgccacttc tccacttcct
    18181 gtttcattat cgttccttaa gaaagtcaca caggaatagt atcgaattct tgccgagctt
    18241 gactcaatta gttcagtcag tttcaggata ttagtcatct ctacattgat tatgagtatc
    18301 cagaaattca ttaaatattc tgacaaatgc tctttcccta aactaccccc ataaaaaaaa
    18361 cccgccgaag cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc
    18421 ccagacctgc gttcagcagt tctgccaggc tggcagatgc gtcttctgca gtcacctgcg
    18481 gtgcagccgg agcttcaccc gcagcacggc gacgcatacg atcctggtga tacgcgtaac
    18541 cggtaccagc cgggatcaga cgacccacga taacgttctc tttcaggccg cgcagttcgt
    18601 cgcgtttgcc cgcaacggct gcttcggtca gcacgcgagt ggtctcctgg aacgatgccg
    18661 cggagatgaa ggactcggtt gccagagacg ctttggtgat acccagcaga tcgcgggagt
    18721 aagttgcacc cactttgccg ttcgcttcca gttcgcggtt tgcgatcttg acgcgagagt
    18781 attcaacctg ttcgccttcc aggaagtcgg agctgcccgc gttaacgatg gtagctttac
    18841 gcagcatctg acgaacgata acttcgatgt gtttatcgtt aatcttaacg ccctgcagac
    18901 ggtatacgtc ctgtacttcg ttaacgatgt aacgagtaac agcatgaaca ccacgcagac
    18961 gcagaatgtc gtgcggcgct tccggaccgt cggaaattac gtcaccacgt tctacacgtt
    19021 caccttcgaa cacgttgagc tgacgccatt tcggaatcat ctcttcgtac ggatcgctac
    19081 catctaccgg ggtgataacc agacgacgtt tacctttggt ttctttaccg aaggaaacga
    19141 taccgctgat ttcagccagg attgccggct ctttcggacg acgtgcttcg aacaggtccg
    19201 caacgcgcgg cagaccaccg gtgatgtcct tggtaccgcc ggattcctgc ggaatacgcg
    19261 ccagggtgtc accagagctg atctgtacgc catcttccag ctgaacaatc gctttacccg
    19321 gcaggaagta ctgcgctggc atatcggtac ccgggatcag aacgtcgtta ccctgagcat
    19381 caacgatttt cagtgccgga cgcagatctt taccacctgc ggtacgttct gcggaatcca
    19441 gaaccaccag cgaagacaga ccggtcaatt cgtcggtctg acgcgtaatg gtctgaccgt
    19501 cgatcatgtc agtaaagcgt acaaaaccgc ttacttcggt gataaccggc atggtgtgcg
    19561 ggtcccagtt tgcaacggtt tcgccgccag caacctgttc gccatcgcct ttcgccagta
    19621 ccgcaccgta aggtactttg tagctttctt tggtacgacc gaattcgtcg atcagtttca
    19681 gttcggtgtt acgggaagtg ataaccagtt taccgctgga gttcacaacc gacttcacgt
    19741 tgctgagctt gatgctacct ttgtttttca cctggatgct ggattcagca gccgcacgag
    19801 atgccgcacc accgatgtgg aacgtacgca tggtcagctg tgtacccggt tcaccgatgg
    19861 actgtgccgc gataacaccg attgcttcac ctttgttgat gatgtggcca cgcgccaggt
    19921 cacgaccgta gcagtgcgca catacaccaa agtcggtgtc acaagataca acagaacgta
    19981 ctttaaccgc gtcgacagag ttctcttcca gcaggtcaca ccactgttcg tgcagcagcg
    20041 tgttgcgcgg aacgaggata tcagcagtac ccggcttcag aacgtcttca gcagttacac
    20101 gacccagtac gcgatcgcgc agcggctctt taacgtcacc accttcgata accggagtca
    20161 tcatgatacc ttcatgagta ccacaatcgt cttcggtaac caccaggtcc tgcgccacgt
    20221 caaccagacg acgagtcagg taaccggagt tcgcagtttt cagtgcggta tccgccagac
    20281 ctttacgagc accgtgggtg gagatgaagt actggagtac gttcagacct tcacggaagt
    20341 tcgcggtgat tggcgtttcg atgatggagc catccggctt cgccatcaga ccacgcatac
    20401 cagcgagctg acgaatctgt gccgcagaac cacgcgcacc ggagtcggcc atcatgtaga
    20461 tgctgttgaa ggaaacctgc ttctcttcct gaccgtcacg gttaatcacg gtttcagttt
    20521 gcaggttatc catcatcgct ttggatacac gatcgttcgc cgcagcccag atatcgataa
    20581 ctttgttgta gcgttcgccc gcagttacca gaccagactg gaactgctcc tgaatttcag
    20641 caacttctgc ttctgcctcg gagatgattt cgtgtttctt ctccgggatg accatgtcat
    20701 cgataccaac agatgcacca gaacgcgctg cataggcgaa gccggtgtac atgatctggt
    20761 ccgcaaaaat aacggtcggt ttcagaccga gaatgcggta gcaggtgttc agcattttgg
    20821 agattgcttt tttacccagc gcctggttga cgatggagta aggcagacct ttcggtacaa
    20881 tcatccacag aatggcacgg ccaacagtcg tgtctttcag gctggttttc gctactaatt
    20941 caccgttagc atctttttca tactcggtga tacgcacttt aacgcgcgca tgcagagaag
    21001 ccagaccaga gcgatacaga cgttctgctt ctttcgggcc agtcagcacc atgccttcgc
    21061 ctttggcgtt aacacagtca cgggtcatgt agtacagacc cagtacaacg tcctgagacg
    21121 gaacgatgat tggttcgccg ttcgccgggg aaaggatgtt gttggtagac atcatcagcg
    21181 cacgcgcttc cagctgggct tccagcgtca gcggtacgtg aactgccatc tggtcaccat
    21241 cgaagtcggc gttatatgcc gcacaaacca gcgggtgcag ctggatagct ttaccttcga
    21301 tcagtaccgg ttcaaatgcc tggataccca ggcggtgcag agtcggtgca cggttcagca
    21361 gtaccgggtg ttcgcggata acttcgtcca ggatatccca aacgacagct tcttcgcgct
    21421 caaccatttt cttcgcagct ttaatggtgg tagcaagacc acgcagttcc agcttgccgt
    21481 agatgaacgg tttgaacagc tccagcgcca ttttcttcgg cagaccgcac tgatgcagac
    21541 gcaggtatgg acctacggtg attacagaac gaccggagta gtcaacacgc ttaccgagca
    21601 ggttctgacg gaaacgaccc tgtttacctt tgatcatgtc ggccaaagat ttcagaggac
    21661 gtttgttaga accggtgatc gcacgaccgc gacgaccgtt atccaacagg gcgtcaaccg
    21721 cttcctgcag catacgtttt tcgttacgta cgatgatgtc cggcgcagcc agatccagca
    21781 gacgcttcag acggttgtta cggttaatga cgcgacgata cagatcgttc aggtcagaag
    21841 tcgcgaaacg accaccatcc agcggaacca gcggacgcag atctggcggc agtaccggca
    21901 gaacggtcag gatcatccac tctggtttgt taccagactg aacgaacgct tccagcagtt
    21961 tgatacgctt ggtcagcttt ttacgcttgg tttcggagtt ggtttcgttc agctcttcac
    22021 gcagctgttc acactcttgc tccagatcca tgctcttcag cagggcctgg attgcttccg
    22081 cacccatctt cgcgtcgaat tcgtcaccga actcttccag cgcgtccaga tactgctctt
    22141 cagtcaggat ctgctgacgc tccaggttgg tcataccgcc ttcaataacc acataggatt
    22201 caaagtacag tacgcgttcg atatcgcgca gcggcatatc gagcagcagg ccgatacggg
    22261 acggcagcga tttcaggaac cagatgtgcg cagtcgggga agccagttcg atgtggccca
    22321 tacgctcacg gcgtacttta gtctgggtca cttcaacgcc gcacttctca cagatgacgc
    22381 cacggtgttt caggcgcttg tacttaccgc acaggcactc gtaatctttt accggcccaa
    22441 agatacgggc gcagaaaagg ccgtcacgtt ctggtttgaa cgtacggtag ttgatggttt
    22501 ccggcttttt aacttcaccg aaagaccatg aacggatcat gtctggcgaa gccagagcaa
    22561 ttttgatcgc atcaaactct tcggttttag tctgcgcttt cagaaacttt aataaatctt
    22621 tcacggattt gctcccgtcg gagttagcac aatccgctgc cgggttttac cccgacagca
    22681 gtgacctgtt tgagcgagaa ttactcgtct tccagttcga tgttgatacc cagcgaacga
    22741 atctctttca acaatacgtt gaaggattct ggcatgcccg gctccatctg atggttgccg
    22801 tccacgatgt ttttatacat cttagtacga ccgttcacgt catcagactt aacggtgagc
    22861 atttcctgca gggtgtatgc tgcgccgtat gcttccagcg cccacacttc catctccccg
    22921 aaacgctgac caccgaactg tgccttacca cccagcggct gctgagtaac caggctgtaa
    22981 gaaccggtgg aacgcgcgtg catcttgtcg tcgaccaggt ggttcagttt cagcatgtac
    23041 atgtaaccaa cggttaccgg acgttcgaac tgttcaccag tgcgaccgtc gtacaggcgg
    23101 atctgaccag aagtcggcag gtcgccaagt ttcagcagct ctttaatttc tgcttctttc
    23161 gcaccgtcga acaccggcgt tgcgattggc atacctttgc gcaggttttc agccagacgc
    23221 ataacttctt catcgctgaa ggtactcagg tcaactttct gacgaacgtc agcgcccaga
    23281 tcgtacgcac gctggatgaa ttcgcgcagt ttcgcgactt cttgctgctg tttcagcatg
    23341 gcgttgatct tgtcgccgat acctttcgca gccataccca ggtgggtttc gaggatctga
    23401 ccgatgttca tacgagacgg tacgcccagc gggttcagta cgatgtctac cggcgtaccg
    23461 ttttcatcgt aaggcatatc ttcgatcggg ttgatcttag aaattacacc cttgttaccg
    23521 tgacgacctg ccatcttgtc accaggctgg atacggcgtt taaccgccag atatacctta
    23581 acaatcttca gcacgcccgg tgccagatcg tcgccctggg tgattttgcg gcgtttcgct
    23641 tcgagtttct tctcgaactc gtgtttcagt tcgtcatact gctcagccag ctgttccagc
    23701 tgattttgtt tctcttcgtc ggtcaggccc agctccagcc agcgatcgcg cggcagtttg
    23761 tcgagcttct cagcttcaac gccaccggct accagcacag cacggatacg gctgaacaga
    23821 cccgcttcga ggatctgcag ttcttcagac aggtctttct tcgcctgttt gagctgcatt
    23881 tcttcgattt ccagcgcacg tttgtctttt tctacgccat cgcgagtaaa gacctgaacg
    23941 tcgataaccg taccggatac accgtttggt acgcgcagag aagagtcttt aacgtcagac
    24001 gctttctcac cgaagatcgc acgcagcagt ttttcttctg gggtcagttg agtttcacct
    24061 ttcggcgtta ccttaccaac cagaatgtcg ccaccggtca cttccgcacc aatgtaaacg
    24121 ataccggatt catccagttt ggagagcgca gcttcaccca cgttcggaat gtcagcggtg
    24181 atctcttccg gccccagctt ggtgtcacgg gacacacacg ccagttcctg aatgtggatg
    24241 gtggtgaaac ggtcttcctg aacaacacgc tcggatacga ggatggagtc ttcgaagttg
    24301 taaccattcc acggcatgaa cgctacgcgc atgttctgac caagcgccag ttcaccgagg
    24361 tcggtggacg gaccgtctgc cagcacgtcg cctcgttcta ccggttcacc cagagacaca
    24421 cacggcatct ggttgataca ggtgttctgg ttagaacggg tgtatttggt caggttgtag
    24481 atgtcgatac ctgcttcacc cggatacatc tcgtcttcgt taactttgat aacgatacgg
    24541 gaagcatcca cgtactgaac gacaccacca cgtttagcaa ccgcagttac accggagtca
    24601 acggcaacag cacgttccat acccgtacca accagcggct tatcagcgcg cagagtcgga
    24661 acggcctgac gttgcatgtt cgcacccatc aatgcacggt tggcgtcatc gtgttcaagg
    24721 aacgggatca gggacgcacc gacggatacc acctgctggg tggatacgtc catgtagtca
    24781 acctggtcgc ggctgaacaa gctggattcg cctttgctac ggcaagttac caggtcttct
    24841 acgaagtggc cttcttcatc caggttggag ttcgcctggg cgataacgta gttgccttct
    24901 tcgatagcag acaggtagtg aatttcgtca gttacaacac cgtcggtcac tttacgatac
    24961 ggagtctcaa ggaagccgta ttcgttagtc tgtgcgtaca cggacagaga gttgatcaga
    25021 ccgatgttcg gaccttcagg ggtttcgatt ggacatacgc gaccgtagtg agtcgggtgt
    25081 acgtctcgaa cttcgaagcc tgcacgttca cgggtcagac cgcctgggcc gagtgcggag
    25141 atacgacgtt tgtgcgtaat ctcagacagc gggttgttct ggtccataaa ctgagacagc
    25201 tggctggaac cgaagaactc tttcactgct gcggaaatcg gcttggcgtt gatcatatcc
    25261 tgaggcatca gggtatccag atcgcccaga gacagacgct ctttcaccgc acgctctaca
    25321 cgtaccaggc caacacggaa ctggttttcc gccatttcac caacggaacg gatacgacgg
    25381 ttgccgaggt ggtcgatatc atcgacttcg cctttaccgt tacggatatc gatgagcttt
    25441 ttcataacat caatgatgtc gtctttgctc aggataccgg aaccttcgat ttcttcgcgc
    25501 agcagagaac ggttgaactt catacgacca accgcagaca agtcataacg gtcttcggag
    25561 aagaacaggt tctcgaacag gctttcagct gcttcacgag tcggcggctc gccaggacgc
    25621 atcatgcggt agatttctac cagtgcgctc agacggtcgt tagttgggtc gacacgtaag
    25681 gtttcagaga tatatgggcc gtgatccaga tcgttggtga acagcgtttc gatacgcttg
    25741 tgaccagact ggctcagctt agccagcaga tccaggctca gctccatgtt cgcagcacag
    25801 atcagctcgc cggtagactc atcaatatag tctttagcaa ccactttacc tgcgatgtac
    25861 tcaaccggga cttcgatcag tttgacgtcg tctttttcca gctggcgaat gtggcgcgca
    25921 gtgatacggc ggcctttttc tacgtacact ttaccgttag cttcgatgtc gaaagatgcg
    25981 gtttcaccac gcaggcgttc cggcaccagt tccatctgca gcttgttatc acggatttca
    26041 aagataactt tttcaaagaa caggtcgagg atctgctctg tggtgtagtt cagggcacgc
    26101 agaatgatgg tcgcaggcag tttacggcga cggtcaatac gtacgaacag gttgtccttc
    26161 ggatcgaatt cgaagtccag ccaggaacca cggtaaggga tgatacgcgc gttatacagc
    26221 actttacccg aagagtgggt tttacctttg tcggagtcaa agaagacgcc cggactacgg
    26281 tgcagctggg aaacgataac acgctcagta ccgttgataa caaaggtacc gttgtctgtc
    26341 atgagcggaa tttcgcccat gtagacttct tgttctttaa tgtcttttac ggtgccttcc
    26401 ggcgcttcgc gctcatagat caccagacgc agtttaacgc gcagcggtgc ggaataggtc
    26461 acgccacgga tttgacattc ctggacgtca aacaccggtt cgccaaggcg gtagctgacg
    26521 tattgcagct cggaattacc gctgtagctc tgaatcggga atacggaacg gaaagcagct
    26581 tccagaccat actgcccttc aggatcttgc tcgataaatt tctgaaacga gtcaagctgg
    26641 atagaaagga gataaggtac atccagaact tgtggacgtt taccaaaatc cttacgaata
    26701 cgttttttct cggtatagga gtaaaccata gggttcctca gctcgctgac aagtcgaccc
    26761 atctgtccat tgagcggaca gtttgtgcaa cactattttg ttgaccggaa aatggaacac
    26821 tttccgcaat gcctgttgct atcacgctta aaccatttca ttgcgattta cacagaacgg
    26881 acgtcctgtc gcagtatatt aagtcgtcga tagaaacaag cattgaaagg cacagcagta
    26941 gtcaaacagt gtgaaacgct actggcgcct tacagcgcaa aaaggctggt gactaaaaag
    27001 tcaccagcca tcagcctgat ttctcaggct gcaaccggaa gggttggctt atttaacttc
    27061 aacttcagcg ccagcttctt ccagagcttt tttcagtgct tctgcgtcgt ctttgctcac
    27121 gccttctttc agagcagccg gtgcagattc taccaggtct ttagcttctt tcagacccag
    27181 gccagttgcg ccacgtactg ctttgataac agcaactttg ttagcgccag cagctttcag
    27241 aattacgtcg aattcagttt tttcttcagc agcttcaacc gggccagcag ctacagctac
    27301 agcagcagca gcggaaacac cgaatttttc ttccattgca gagatcagtt ctacaacgtc
    27361 cattacagac atagctgcaa ctgcttcaat gatttgatct ttagtgatag acatttaaa
//
LOCUS       DAYPHN010000053        27394 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000053 DAYPHN010000000
VERSION     DAYPHN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.053"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACG0BW_002150"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACG0BW_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFK7259105.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACG0BW_002151"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACG0BW_002151"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFK7259106.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACG0BW_002152"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACG0BW_002152"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFK7259107.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGKILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACG0BW_002153"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACG0BW_002153"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFK7259108.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACG0BW_002154"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACG0BW_002154"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFK7259109.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6617..7111
                     /gene="lrp"
                     /locus_tag="ACG0BW_002155"
     CDS             6617..7111
                     /gene="lrp"
                     /locus_tag="ACG0BW_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFK7259110.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7246..11352
                     /gene="ftsK"
                     /locus_tag="ACG0BW_002156"
     CDS             7246..11352
                     /gene="ftsK"
                     /locus_tag="ACG0BW_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFK7259111.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPSVASVDVAPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYATAPEQSAQQSYYAPAPEQSVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPVAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQQQYQQLQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11511..12122
                     /gene="lolA"
                     /locus_tag="ACG0BW_002157"
     CDS             11511..12122
                     /gene="lolA"
                     /locus_tag="ACG0BW_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFK7259112.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12133..13476
                     /gene="rarA"
                     /locus_tag="ACG0BW_002158"
                     /gene_synonym="mgsA"
     CDS             12133..13476
                     /gene="rarA"
                     /locus_tag="ACG0BW_002158"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFK7259113.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13567..14859
                     /gene="serS"
                     /locus_tag="ACG0BW_002159"
     CDS             13567..14859
                     /gene="serS"
                     /locus_tag="ACG0BW_002159"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFK7259114.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15098..17542
                     /gene="dmsA"
                     /locus_tag="ACG0BW_002160"
     CDS             15098..17542
                     /gene="dmsA"
                     /locus_tag="ACG0BW_002160"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFK7259115.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMQHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17553..18170
                     /gene="dmsB"
                     /locus_tag="ACG0BW_002161"
     CDS             17553..18170
                     /gene="dmsB"
                     /locus_tag="ACG0BW_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFK7259116.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18172..19035
                     /gene="dmsC"
                     /locus_tag="ACG0BW_002162"
     CDS             18172..19035
                     /gene="dmsC"
                     /locus_tag="ACG0BW_002162"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFK7259117.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIHTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19071..19697)
                     /gene="ycaC"
                     /locus_tag="ACG0BW_002163"
     CDS             complement(19071..19697)
                     /gene="ycaC"
                     /locus_tag="ACG0BW_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFK7259118.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20012..21160
                     /gene="ycaD"
                     /locus_tag="ACG0BW_002164"
     CDS             20012..21160
                     /gene="ycaD"
                     /locus_tag="ACG0BW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259119.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLTWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLFLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21370..22800
                     /gene="ycaM"
                     /locus_tag="ACG0BW_002165"
     CDS             21370..22800
                     /gene="ycaM"
                     /locus_tag="ACG0BW_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFK7259120.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANDK"
     gene            complement(22801..22911)
                     /locus_tag="ACG0BW_002166"
                     /pseudo
     CDS             complement(22801..22911)
                     /locus_tag="ACG0BW_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(23010..23750)
                     /gene="pflA"
                     /locus_tag="ACG0BW_002167"
     CDS             complement(23010..23750)
                     /gene="pflA"
                     /locus_tag="ACG0BW_002167"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFK7259121.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(23942..26224)
                     /gene="pflB"
                     /locus_tag="ACG0BW_002168"
     CDS             complement(23942..26224)
                     /gene="pflB"
                     /locus_tag="ACG0BW_002168"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFK7259122.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(26279..27136)
                     /gene="focA"
                     /locus_tag="ACG0BW_002169"
     CDS             complement(26279..27136)
                     /gene="focA"
                     /locus_tag="ACG0BW_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFK7259123.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6764 a         6746 c         7193 g         6691 t
ORIGIN      
        1 ttgctgccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta tggatttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattg aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgacg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagtttacc gccatgacca ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat agagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgact cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc atgcatcatt tctgcaagca attcaaggat
     1681 ctgactttcg gttgtggcat ctaagccttc tgtaggttca tccagcaata ccagcggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cccaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgcca gagcctcatc actactgcca ggtgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct tgccttgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga attgaacatc ccgtaacgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaagt cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtgag ttcagattga cgacgttgcg cttccagcca
     2521 ctgaatttcg gtattctcta actgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagt ggtggcataa ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtcct gcaggggaga ggggcagcaa tttgctgaag gtataaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaatagcgcc cggcagtacg
     3001 ggtgattgct gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gctaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcatta agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaatgct cgggccaccg ccacgcgctg tgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcac tgacccaggc
     3601 gttatccagc gctgtttgta actcgtgttc gctggcatca ggtcgtgcca gtagtacgtt
     3661 atcccgtagt gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattcc ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccagcca gcgttttacc
     3901 ttctggtgat gtaataaaca gatcctcggc ttcaatggtc accggatcgg ttgatgctaa
     3961 ttccgcctca ccacgttgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcgcca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactcc ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 atagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagaa aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggagcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atagccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgcc ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgagga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cagcagatgt aatagcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac gcccatctga tcgccggtca cctcttccag cgtacggttg
     5461 gtgtgtagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aagtattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gatggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagta
     5761 tattcgccgc tatcgccagt cagacggaac ggacgattct gcaaatcaac tttgttgatg
     5821 tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcagaattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagacggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccaattaaa tgatgaataa acgcctctgg taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 aacagtattc ttatatgcgc ataaccatgc atgtaaatac catgtttacc gtgctagtga
     6421 aatctacgta tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg
     6481 gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg
     6541 tgacgggtag cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata
     6601 cagagagaca ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat
     6661 cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc
     6721 taaacgtgtg ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca
     6781 agggtttatt cagggctata cggcgctgct gaacccccat tatctggatg catcacttct
     6841 ggtattcgtt gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac
     6901 cgctgtacaa aaacttgaag aaattcagga gtgtcattta gtatccggtg atttcgacta
     6961 cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt aagttgctgg gggaaaccct
     7021 gctgcgtctg cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca
     7081 gagtaatcgt ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt
     7141 tgattacact cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc
     7201 gttttagcat cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac
     7261 actgaagaca aagaagtcac attgacaaag ttaagtagcg gccgccgcct tctggaggcg
     7321 ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagctttaac
     7381 ccttcggacc ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg
     7441 atgcccggtg cgtggctggc agatacgctg ttctttattt ttggcgtgat ggcttacacc
     7501 attcccgtca ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa
     7561 tacattgatt attttgccgt ttcgctacgc atcattggcg ttttggcgct catccttacc
     7621 tcctgtggtc tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc
     7681 attggcagct tactaagcac tacgctacaa ccactgctac acagtagcgg gggaactatt
     7741 gcgctgctct gcgtttgggc agcgggcctg acgctgttca ccggttggtc atgggtgacc
     7801 attgctgaaa aactcggcgg ctggatttta aatattctta ccttcgccag taatcgtacc
     7861 cgtcgcgatg atacctgggt cgatgaagat gagtatgaag atgacgaaga gtatgaagat
     7921 gaaaatcacg gcaaacagca tgaatcacgc cgtgctcgta ttcttcgcgg cgcgctagcg
     7981 cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg
     8041 ttgttctccg gtaagcggat ggatgatgac gaagagatta cctacactgc acgcggtgtg
     8101 gctgccgacc ctgacgacgt cctcttttcg ggcaatcgtg caacgcagcc agaatatgac
     8161 gagtacgatc cattattaaa cggtgcgcca attaccgaac ctgttgctgt agcagctgct
     8221 gctaccacgg cgacgcaaag ctgggctgca ccggttgaac ctgtgactca gacgccatct
     8281 gttgcctctg ttgatgttgc acctgcacaa cctacagtag cctggcagcc tgtaccgggg
     8341 ccacaaacgg gagagccggt gattgctcct gcaccggaag gttacccaca gcagccacaa
     8401 tatgcgcagc ctgcagtgca atataatgag ccgctgcaac aacctgtaca gccacagcag
     8461 ccgtattatg cgcctgcggc ggaacaacct gttcagcaac cgtattacgc aactgcgccg
     8521 gaacagtctg cgcaacagtc gtattacgcc cctgcgccag aacaatcggt ggcaggtaac
     8581 gcctggcaag ccgaagagca gcaatccact tttgctccac agtctacata ccagactgag
     8641 caaacttatc agcagccagt cgctcaggag ccgttgtacc aacagccgca acctgttgaa
     8701 cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg
     8761 ctttactact ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc
     8821 tggtatcaac cgattccaga accggttaaa gaaccagaac cgatcaaatc ttcgctgaaa
     8881 gcaccttctg ttgcagcagt acctccagta gaagccgctg ccgctgtttc tccgctggca
     8941 tctggcgtga aaaaagcgac actggcgacg ggggctgccg caaccgttgc cgcgccagtc
     9001 ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca aagaggggat tggcccgcag
     9061 ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg aattggcgtc ttacggtatt
     9121 aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg aagcccagcg caatcagtac
     9181 gattctggcg atcagtacaa cgatgatgaa atcgatgcga tgcagcagga tgaactggca
     9241 cgtcagttcg cccagacaca gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc
     9301 gtaaacgcag aagatgcaga tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa
     9361 actcaacaac aacgttattc cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat
     9421 gattttgaat tttcgccaat gaaagcgttg ctggatgatg gtccacacga accgttgttt
     9481 acgccaattg ttgaacctgt acagcagccg caacaaccgg ttgcaccgca gcagcagtat
     9541 cagcagccac aacagccggt tgcgccacag cagcaatatc agcagccgca acagccggtt
     9601 gcgccgcagc agcagtatca gcagccacaa cagcaggttg cgccgcagcc acaatatcag
     9661 cagccgcaac aaccggttgc gccacagcag caatatcagc agccgcaaca gccggttgcg
     9721 ccgcagcagc agtatcaaca gctgcaacaa ccggttgcac cgcagccgca gtatcagcag
     9781 ccacaacagc cagttgcgcc acagccgcag gataccctgc ttcatccgct gttgatgcgc
     9841 aatggtgaca gccgtccgtt gcataaaccg acgacgccgc tgccttctct ggatttgctg
     9901 acgccgccgc cgagcgaagt ggagccggta gatacctttg cgcttgaaca aatggcgcgc
     9961 ctggtggaag cgcgtctggc tgatttccgt attaaagccg atgtcgtcaa ttactctccg
    10021 gggccggtta tcactcgctt tgaacttaac ctggcaccgg gcgtaaaagc ggcgcgcatt
    10081 tctaacttgt cacgggacct tgcccgttca ctttcgacgg tggcggtgcg tgtcgttgaa
    10141 gttattcctg gcaaacccta tgtaggtctg gagttaccga ataaaaaacg acaaaccgtt
    10201 tatctgcgcg aagtattgga taacgccaaa ttccgcgata atccgtcgcc attaaccgtg
    10261 gtgctgggta aagatatcgc cggtgagccg gtggttgccg atctggcgaa aatgccgcac
    10321 ttgttggttg cggggactac cggttccggt aagtctgtcg gggtgaacgc gatgatcctg
    10381 agcatgcttt ataaagcaca gccagaagat gtgcgtttca tcatgatcga cccgaaaatg
    10441 ctggagcttt cggtttatga aggcattccg catctgttaa cggaagtcgt taccgacatg
    10501 aaagatgctg ccaacgcttt gcgctggtgc gtaaatgaaa tggaacgtcg ttataagctg
    10561 atgtccgccc taggtgtgcg taatctggcg ggttataacg aaaaaattgc tgaagccgat
    10621 cgcatgatgc gtccgattcc agacccgtac tggaagccgg gtgacagtat ggatgcccag
    10681 catccggtgc tgaaaaaaga accgtacatt gtggtgttgg tggacgaatt tgccgacctg
    10741 atgatgacgg taggtaaaaa agtggaagag cttatagcac gtctggcgca aaaagcccgt
    10801 gccgcgggta tccacctcgt actggcaacg cagcgtccat cggttgatgt tattactggt
    10861 ctgattaaag cgaatattcc gacccgtatc gcctttaccg tatccagtaa gattgactca
    10921 cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg gtatggggga tatgctttac
    10981 tctgggccga actccacgtt gccggtacgt gttcatggtg cttttgttcg cgatcaggaa
    11041 gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc cacagtatgt tgatggcatc
    11101 acctccgaca gtgaaagcga aggtggcgcg ggcggtttcg atggcgctga agaactggat
    11161 ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac gcaaagcatc aatttctggc
    11221 gtacagcgtc agttccgcat tggttataac cgtgcagcgc gtattatcga acagatggaa
    11281 gcgcagggga ttgtcagcga acaggggcac aacggtaatc gtgaagtgct ggccccaccg
    11341 ccgtttgact aattaatgca tcgtaggccg gataaggcgc ggtagcgttg catccggcac
    11401 tctatcaact gaaaattcag tattttcttc tttcctcaag ctgattatta gcctggaata
    11461 gagagtagag ggaactcccg gtcgggagtg acgtaatttg aggaataatg atgaaaaaaa
    11521 ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg gctgatgccg
    11581 caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc ttcacacaaa
    11641 aagtgactga cggtagcggc gcggcggtgc aggaaggcca gggcgatctg tgggtgaaac
    11701 gtccaaactt attcaactgg catatgactc aacctgatga aagcattctg gtttctgacg
    11761 gtaaaacact gtggttctat aacccgttcg ttgagcaagc tacggcaacc tggctgaaag
    11821 atgccaccgg taatacgccg tttatgctga ttgcccgcaa ccagtccagc gactggcagc
    11881 agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc agcaatggca
    11941 atctgaagca gttcaccatt aacgtgggac gtgatggcac gatccatcag tttagcgcgg
    12001 tggagcagga cgatcagcgc agcagttatc agctgaaatc ccagcaaaat ggggctgtgg
    12061 atgcagcgaa atttaccttc accccgccgc aaggcgtcac ggtagatgat caacgtaagt
    12121 agaggcacct gagtgagcaa tctgtcgctc gatttttcgg ataacacttt tcaacctctg
    12181 gccgcgcgta tgcggccaga aaatttagca cagtatatcg gtcagcaaca tttgctggct
    12241 gcggggaagc cgttgccgcg tgcaatcgaa gccgggcatt tgcattcgat gatcctctgg
    12301 gggccaccgg gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct
    12361 gatgtggaac gtatttctgc cgttacttct ggcgtgaaag agattcgcga ggcgatcgag
    12421 cgcgctcggc aaaaccgcaa tgctggtcgc cgcactattc tttttgttga cgaagttcac
    12481 cgtttcaaca aaagccagca ggatgcgttt ctgccacata ttgaagacgg caccatcact
    12541 tttattggtg caaccactga aaacccgtca tttgagctta attcggcact gctttcccgt
    12601 gcccgtgtct atctgctgaa atccctgagt acagaggata ttgagcaagt actgactcag
    12661 gcgatggaag acaaaacccg tggctatggt ggtcaggata ttgttctgcc agatgaaaca
    12721 cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa
    12781 atgatggcgg atatggccga agtcgatgat agcggtaagc gggtcctgaa gcctgaatta
    12841 ctgaccgaaa tcgccggtga acgtagcgcc cgctttgata acaaaggcga tcgcttttac
    12901 gatctcattt ccgcactgca taagtcggta cgtggtagcg cacccgatgc ggcgctgtac
    12961 tggtatgcac gaattattac tgccggtggc gatccgttat atgtcgcgcg tcgctgtctg
    13021 gcgatagcgt cggaagatgt gggtaatgct gatccacgag cgatgcaggt ggcaattgcg
    13081 gcctgggatt gctttacccg agttggcccg gcggaaggtg aacgcgccat tgctcaggcg
    13141 attgtttacc tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt taaagccgcg
    13201 ctggcggatg ctcgcgaacg tccggattat gacgtgccgg ttcatttgcg taatgcgccg
    13261 acgaaattaa tgaaggaaat gggctacggg caggaatatc gttacgctca tgatgaagca
    13321 aacgcttatg ctgccggtga ggtttatttc ccgccggaaa tagcacaaac acgctattat
    13381 ttcccgacaa acaggggcct tgaaggcaag attggcgaaa agctcgcctg gctggctgaa
    13441 caggatcaaa atagccccat aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt
    13501 actgtatccc tgtggtcgca ggctgtggcc acatctccca tttaattcga taagcacagg
    13561 ataagcatgc tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg
    13621 gcacgccggg gctttaagct ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa
    13681 gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta actcccgatc gaaatccatt
    13741 ggccaggcga aagcgcgcgg ggaagatatc gagcctttac gtctggaagt gaacaaactg
    13801 ggcgaagagc tggatgcagc aaaagccgag ctggatgctt tacaggctga aattcgcgat
    13861 atcgcgctaa ccatccctaa cctgcctgca gatgaagtgc cagtaggtaa agacgaaaat
    13921 gacaacgttg aagtcagccg ctggggcacc ccgcgtgagt ttgactttga agttcgtgac
    13981 catgtgacgc tgggtgaaat gcactctggc ctcgactttg cagccgcagt taagttgacc
    14041 ggttcccgct ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc actgtcgcag
    14101 tttatgctgg atctgcatac cgaacagcat ggctacagtg agaactatgt tccgtacctg
    14161 gttaaccagg acacgctgta cggtacgggt cagctgccga aatttgctgg cgatctgttc
    14221 catactcgtc cgctggaaga agaagcagac accagtaact atgcgctgat cccaacggca
    14281 gaagttccgc tgaccaacct ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt
    14341 aagatgaccg cccacacccc atgcttccgt tctgaagccg gttcatatgg tcgtgacacc
    14401 cgtggtctga tccgtatgca ccagttcgac aaagttgaaa tggtgcagat cgtgcgaccg
    14461 gaagactcaa tggcggcgct ggaagagatg actggtcatg cagaaaaagt cctgcagttg
    14521 ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg acatgggttt tggtgcttgc
    14581 aaaacttacg acctggaagt atggatcccg gcacagaaca cctaccgcga gatctcttcc
    14641 tgctccaacg tctgggattt ccaggcgcgt cgtatgcagg cacgttgccg tagcaagtcg
    14701 gacaagaaaa cccgtctggt tcataccctg aacggttctg gtctggctgt tggtcgtacg
    14761 ctggttgcgg ttatggaaaa ctatcagcag gctgatggtc gtattgaagt accagaagtt
    14821 ctgcgtccgt atatgaacgg actggaatat attggctaat acccaatttt tctgaatcta
    14881 aaaagcgcct gcgggcgctt tttttgtctc cctttgatac cgaacaataa ttactcctca
    14941 cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt tcaaattatt
    15001 cgatgtatac aagcctatat agcgacctgc tatagaaata attacacaat acggtttgtt
    15061 actggaatca atcgtgagca agcttgagtg agccattatg aaaacgaaaa tccctgatgc
    15121 ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct
    15181 ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg ctgtcgatag
    15241 cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag ttaactgtgg
    15301 tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg ttgaaacgga
    15361 taatactggc gatgacaact atgacggcct gcaccaggtg cgcgcctgtc tgcgtggtcg
    15421 ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa tatccgatga aacgagtcgg
    15481 ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg acatcatcgc
    15541 gaccaatatg cagcgcctga tcaaagagta cggcaacgaa tccatctatc tgaactatgg
    15601 caccggtacg ctgggtggca ccatgacccg ctcctggccg ccgggaaata ccctggtcgc
    15661 tcgcctgatg aactgctgcg gcggttatct gaaccattac ggtgactact cttccgcgca
    15721 aattgcggaa ggtttgaact atacctacgg cggctgggca gatggtaaca gcccgtcgga
    15781 tatcgaaaac agtaaactgg tagtgctgtt tggtaataac cctggcgaaa cgcgaatgag
    15841 tggcggtggg gtgacttact atcttgaaca ggcgcgtcag aaatctaatg ctcgcatgat
    15901 catcatcgat ccgcgctata ccgacaccgg agccgggcgc gaagatgagt ggatccctat
    15961 tcgtccggga acagatgccg cactggttaa cggtctggcg tatgtcatga tcaccgaaaa
    16021 cctggtggat caggcattcc tcgataaata ttgcgttggc tacgacgaga aaacactgcc
    16081 agccagtgcg ccgaaaaatg gtcactataa agcttatatt ctgggtgaag ggccagatgg
    16141 cgtggctaaa acgccggaat gggcctcgca aatcaccggt gttccggcag acaaaatcat
    16201 caaactggct cgtgaaatcg gtagtaccaa accggcgttt atcagccagg gatggggccc
    16261 gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac
    16321 cggtaacgtt ggtattaacg gggggaacag cggcgcgcgt gaaggttcat acagcttacc
    16381 gtttgtccgt atgccgaccc tcgaaaaccc gatccagacc agcatttcga tgtttatgtg
    16441 gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg tgcgcgggaa
    16501 agataagctg gatgtgccga tcaaaatgat ctggaactat gctggtaact gcctgattaa
    16561 ccagcattct gaaatcaacc gtacccatga aatccttcag gatgataaga agtgcgagct
    16621 gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca tcctgctgcc
    16681 tgactgcacc gcttccgaac agatggactt tgcactggat gcatcctgcg ggaatatgtc
    16741 ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga ccatctatga
    16801 aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg aaggccgtac
    16861 ccaggaagag tggatgcagc atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc
    16921 aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac aagggcatca
    16981 cgttgcttat aaagccttcc gtgaagatcc gcaggcaaat ccactgacca cgccatcggg
    17041 taaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg aattgccaga
    17101 aggcgatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt atcaggatcc
    17161 gctgaacaaa cagtatccgc tgcagcttac gggtttccac tataaatctc gcgttcactc
    17221 aacttacggc aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt ggatcaaccc
    17281 gcttgatgcc caaaaacgcg gtatcaacaa cggcgataaa gtcaggatct ttaacgatcg
    17341 tggtgaggtt catattgagg cgaaagtgac gccacgaatg atgccgggtg tggtcgcact
    17401 gggtgaaggt gcctggtatg acccggatgc aaaacgtgtc gataagggcg gttgtatcaa
    17461 cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac atacaaacct
    17521 tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga ttttttattg
    17581 attccagccg ttgcaccggt tgcaaaacct gcgagctggc ctgtaaagac tataaagatc
    17641 tgacgccgga agtcagcttc cgccgcattt atgaatatgc tggcggtgac tggcaggaag
    17701 ataacggtgt ctggcatcag aacgtgtttg cctactatct gtcgatttca tgtaaccact
    17761 gcgaagatcc ggcttgtact aaagtctgcc cgagtggtgc gatgcataaa cgtgaagatg
    17821 gttttgttgt agtcgatgaa gatgtgtgca ttggctgccg ctactgccat atggcttgcc
    17881 cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc gatggttgtt
    17941 atgaccgtgt tgcggagggg aaaaagccga tctgtgttga atcttgcccg ctgcgggcgc
    18001 tggatttcgg ccctatcgac gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc
    18061 cgttgccgcg agctcacttt accaaaccga atattgtgat caaacccaat gccaacagcc
    18121 gcccgaccgg ggataccact ggctatctgg caaacccgaa ggaggtgtga gatgggaagt
    18181 ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt agcgggtggt
    18241 tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt
    18301 gttattgcct gcatgtttgg tttatgggtg ctgatgggca ttggctttat cgcctctatg
    18361 cttcatcttg gttcaccgat gcgcgccttt aactcgctca accgggtagg ggcttcagca
    18421 ctcagtaacg aaatcgccag cggttcgatc ttttttgctg ttggtggcat cggctggttg
    18481 ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc tgtggctgat agtgacgatg
    18541 gttcttggcg tcatctttgt ctggatgatg gtgcgtgtgt ataacagcat tcataccgtt
    18601 ccgacctggt acagcatctg gacgccgatg ggcttcttcc tgacgatgtt tatgggcggc
    18661 ccgttactgg gttacctgtt gctgagtctg gctggcgtcg atggctgggc gatgcgtctg
    18721 ctgccagcca tttctgtact ggcactggta gtgagtggtg tgatgtcagt gatgcagggc
    18781 gcagagctgg cgactattca tagttctgtg cagcaggccg cagcgctggt gccggactat
    18841 ggtgcgctga tgtcctggcg gatcgtgctt ttggctgttg ccctgtgttt gtggattgca
    18901 ccacagctaa aaggttatca gcctgcggtt ccgctactat ccgtctcatt cattctgctt
    18961 ctggcaggag aattaatcgg ccgcggcgta ttctatggtt tgcatatgac cgtggggatg
    19021 gccgtcgcaa gctaatcagt aacaaccggg gtttcgaccc cggttctctt ttatttctgc
    19081 ttcgttaatg tgtcataact ggtcatcaaa ttacgataat caggaatgtg gttagagaac
    19141 aacgtcgcca gtccttcaat atcattacgc cagtcacggt gcagctcgca agccacgccg
    19201 aaccatgtca tcaattgcgc accagcttgc gacatgcgat cccacgccga atgccgggta
    19261 atttcattaa aggtccctga tgcatcagtc acgacaaata catcaaaccc ctcttcaatg
    19321 gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat aattaactgt
    19381 tttttacctg tcgctttgac agcttttaca aaatcttcgt tatcccaggc gttaatattg
    19441 cccgggcgag caatgtaagg tgcatcgggg aactgtgctt tcagttctgg aactaatggg
    19501 ccgttaggac cggtttcaaa actggtggta agaatagtag gtaagttgaa atacttagcc
    19561 aggtcgccca gcgccagcac gttattttta aacttatcgg gttcgatatc ccgtacaagg
    19621 gaaagtaaac cagcctggtg atcaacaagc aaaacggcag catcattttt atcaagacga
    19681 acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgtct gaaatcggtc
    19741 agacgcgatg cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa
    19801 atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga
    19861 gattaatcgt ctctccagca ttagcgttat gaatgagtaa tataaatcga taatacctca
    19921 gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca
    19981 tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct
    20041 tttgctgtct ggcctgcttt tgttgactct ggcgatcgca gtgttaaata cactcgtgcc
    20101 gctttggctc gcccaggaac atatgtccac atggcaggta ggcgttgtca gctcatccta
    20161 ttttaccggc aacctggtcg gtacattgct gacagggtat gtcattaagc gcattggctt
    20221 taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg gccttggcct
    20281 gatgattgga ttctggagct ggttgacttg gcgttttgtc gccggcgtcg gctgtgccat
    20341 gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg
    20401 tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc agttactggt
    20461 cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg gttacaggtt tgacgttggc
    20521 agggatctta ccgctgttgt ttacgcgtgt gctgaatcag caggctgaaa accatgattc
    20581 gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat
    20641 tatctcagga atcgttctgg gatctctata tggcctgatg ccgctgttcc tcaatcacaa
    20701 aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg cgggtatcct
    20761 tggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg tgttgcgtgt
    20821 tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt agccaggcgg cgatggcccc
    20881 agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc
    20941 ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac tgttgagcta
    21001 taccgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga atttctccga
    21061 taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg
    21121 caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa gcagagtgtg
    21181 aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt tattccaaat
    21241 tttattcgtt taaaaataaa atgtgcagca ggttataatt ttgcatttcg ctatttccgc
    21301 acttcttatt tgccgcgcat aatccctcgt tttacagatg cccctttaat tttggcgaag
    21361 gatttgtcta tggctgggaa tgttcaggaa aaacagctgc gatggtacaa cattgcgctg
    21421 atgtctttta tcactgtctg gggttttggc aacgttgtta ataactacgc caaccagggg
    21481 ctggtggttg ttttttcatg ggtgtttatc tttgcgctct atttcacacc ttatgcgcta
    21541 attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag tacctggatt
    21601 aaacacacga tgggacccgg actggcttac ctcgccgcgt ggacctactg ggtagtgcat
    21661 attccctatc tggcacaaaa accccaggca atactgattg cgctcggttg ggcgatgaaa
    21721 ggcgacggtt cgttaatcaa agaatattca gtcgtagcgt tacaggggtt aacgctggtg
    21781 ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa aatcgtcggt
    21841 tctgtggcag ggattgcgat gtttgttatg tcgctcctgt atgtggcgat ggcggtaacc
    21901 gcgcctgcaa ttactgaagt gcatattgcg accacaaaca ttacctggga aacgttcatt
    21961 cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc ggttggcgga
    22021 gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga atttccaaaa
    22081 gggatgttat gcctggcggt gatggttgcg gtttgtgcca ttctgggctc actggcgatg
    22141 gggatgatgt ttgactcgcg taatatcccg gatgacttaa tgactaacgg tcagtattac
    22201 gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt gatttatgcc
    22261 attgcgaata ccctgggaca agtagctgcg ctggtattct cgattgatgc gccgcttaaa
    22321 gtgttattag gcgatgctga cagcaaatat attccagcca gtttatgtcg taccaacgct
    22381 tctggtacgc ccgttaatgg ctattttctg accctggtgc tggtggcgat cctgataatg
    22441 ctaccgactc tcggcattgg tgatatgaac aatctctata aatggctgtt gaatcttaac
    22501 tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc agtcgttcgc
    22561 ctggcgcaga aatataaacc agaatatgtc tttattcgta ataagcctct ggcaatgacc
    22621 gtcgggattt ggtgttttgc ctttaccgcc tttgcctgtt tgacggggat cttcccgaaa
    22681 atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc aacgccgttt
    22741 gtgttggtag gattaggact gatattcccg ctgctggcgc gtaaagcgaa tgataaataa
    22801 ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat aatcgagaaa
    22861 tgcgcgtaag ccgcaggaca tatactggcg gtttgggtaa tacatttgga agctgtgctg
    22921 taagggcgtt agaaatagag ctctgaatta tttatgcaga gcggaataaa aaagcggcaa
    22981 ctcaaaaaag ttgccgcttt acggggaaat tagaacatta ccttatgacc gtactgctca
    23041 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcaagtttg
    23101 tattcttcac ccatcgccac ccatttgtgt ttgccaagtt cgtggtaggg gagaagctcg
    23161 attttctcaa cgttgcccat atcacgggta aattcaccga ggcgatgcgc tgaatcgtca
    23221 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
    23281 agatatttgg cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
    23341 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
    23401 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
    23461 tttttgcagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
    23521 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
    23581 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
    23641 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acccgggccg
    23701 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
    23761 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
    23821 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
    23881 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
    23941 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
    24001 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
    24061 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
    24121 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
    24181 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
    24241 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agaggctact gcacctttct
    24301 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
    24361 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
    24421 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
    24481 ccaggtcaac agccaggtca tctacacgcg gatcgttgtt accaaactgc gggtattcgc
    24541 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
    24601 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
    24661 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
    24721 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
    24781 gatccatgcg ctccatcact tcatcgtagt tcaggagatc gcctttgatc ggttcagact
    24841 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
    24901 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
    24961 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
    25021 tctcatactg cagagaagag gtgtcgatgg acactttagc agcgaatttc ttgaagttca
    25081 gcggcagttt ttcggaccac agaatggtca tgttcggttc cggagacgga cccatggtgt
    25141 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgaccg tcgaggccca
    25201 taccaccgat agattcggtt gcccagatcg ggtcgccaga gaacagttca tcgtattccg
    25261 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
    25321 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
    25381 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
    25441 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
    25501 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
    25561 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgcagagagg
    25621 tgaactgtgc aagtttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
    25681 agtcaccgat gatacgacca cggccataag catctggcag accggtcaga acaccggatt
    25741 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
    25801 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
    25861 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
    25921 tctgcagacc aacaactttt tccaacgctt tgttgatgta gccagcgtcg tgagaggtga
    25981 tggtggaagc aacagcggtg tcaaagtcaa ctggcgcgtg agtgcggttt tccagtttaa
    26041 cgccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
    26101 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
    26161 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
    26221 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
    26281 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
    26341 cgccgataat attaccgatc gtaaccggaa tcaggttatc agtgatgaag ttcattacgg
    26401 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
    26461 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
    26521 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
    26581 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
    26641 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgttgagtc
    26701 cccattgacc attcgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
    26761 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttcgccaac tgaccccagg
    26821 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
    26881 cgcagacaac acaaagaatc agccccagag agaagcaaat accgccaacc agttttgcca
    26941 tgccgaaggg cattgttcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
    27001 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
    27061 tatagacacc tgcctcttcg gccactttgg ccattgctgc aggaagtaaa agatcaaaag
    27121 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
    27181 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
    27241 catggttttt atgcaaatac ggagtaaata tttgattatc caaataaaaa taaattttaa
    27301 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
    27361 agcatcaggc gaataaagta acaatatcga tcgt
//
LOCUS       DAYPHN010000054        27293 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000054 DAYPHN010000000
VERSION     DAYPHN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.054"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            168..926
                     /gene="ydiY"
                     /locus_tag="ACG0BW_002170"
     CDS             168..926
                     /gene="ydiY"
                     /locus_tag="ACG0BW_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFK7259124.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(979..1086)
                     /gene="yniD"
                     /locus_tag="ACG0BW_002171"
     CDS             complement(979..1086)
                     /gene="yniD"
                     /locus_tag="ACG0BW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFK7259125.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1253..3151)
                     /gene="espL1"
                     /locus_tag="ACG0BW_002172"
     CDS             complement(1253..3151)
                     /gene="espL1"
                     /locus_tag="ACG0BW_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFK7259126.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAFLPL
                     LPEGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPITQELLSKKMVMDILAMKTRDGEPGLYAAMENNHP
                     LCVTRFLSKVYGIAVKYNLSKINIMDLLKGATAHGTPALYIAMSKGNKDVVLSYISTL
                     GTFAKKYSFSQCQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            3674..5602
                     /gene="thrS"
                     /locus_tag="ACG0BW_002173"
     CDS             3674..5602
                     /gene="thrS"
                     /locus_tag="ACG0BW_002173"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFK7259127.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5606..6148
                     /gene="infC"
                     /locus_tag="ACG0BW_002174"
     CDS             5606..6148
                     /gene="infC"
                     /locus_tag="ACG0BW_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFK7259128.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6245..6442
                     /gene="rpmI"
                     /locus_tag="ACG0BW_002175"
     CDS             6245..6442
                     /gene="rpmI"
                     /locus_tag="ACG0BW_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFK7259129.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6495..6851
                     /gene="rplT"
                     /locus_tag="ACG0BW_002176"
     CDS             6495..6851
                     /gene="rplT"
                     /locus_tag="ACG0BW_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFK7259130.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6968..7092
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6974..7018
                     /gene="pheM"
                     /locus_tag="ACG0BW_002177"
     CDS             6974..7018
                     /gene="pheM"
                     /locus_tag="ACG0BW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFK7259131.1"
                     /translation="MNAAIFRFFFYFSS"
     gene            7157..8140
                     /gene="pheS"
                     /locus_tag="ACG0BW_002178"
     CDS             7157..8140
                     /gene="pheS"
                     /locus_tag="ACG0BW_002178"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFK7259132.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8155..10542
                     /gene="pheT"
                     /locus_tag="ACG0BW_002179"
     CDS             8155..10542
                     /gene="pheT"
                     /locus_tag="ACG0BW_002179"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFK7259133.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNEETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            10547..10846
                     /gene="ihfA"
                     /locus_tag="ACG0BW_002180"
     CDS             10547..10846
                     /gene="ihfA"
                     /locus_tag="ACG0BW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFK7259134.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            10947..11927
                     /gene="btuC"
                     /locus_tag="ACG0BW_002181"
     CDS             10947..11927
                     /gene="btuC"
                     /locus_tag="ACG0BW_002181"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFK7259135.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWS
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            11990..12541
                     /gene="btuE"
                     /locus_tag="ACG0BW_002182"
     CDS             11990..12541
                     /gene="btuE"
                     /locus_tag="ACG0BW_002182"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFK7259136.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            12541..13290
                     /gene="btuD"
                     /locus_tag="ACG0BW_002183"
     CDS             12541..13290
                     /gene="btuD"
                     /locus_tag="ACG0BW_002183"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFK7259137.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13368..13832
                     /gene="nlpC"
                     /locus_tag="ACG0BW_002184"
     CDS             13368..13832
                     /gene="nlpC"
                     /locus_tag="ACG0BW_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFK7259138.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14079..14792
                     /gene="ydiV"
                     /locus_tag="ACG0BW_002185"
     CDS             14079..14792
                     /gene="ydiV"
                     /locus_tag="ACG0BW_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFK7259139.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTAEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            14855..16291
                     /gene="selO"
                     /locus_tag="ACG0BW_002186"
     CDS             14855..16291
                     /gene="selO"
                     /locus_tag="ACG0BW_002186"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFK7259140.1"
                     /translation="MTLSFVTRWRDELPGTYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQYSAASPLRDEFIDRAAFDDW
                     FARYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(16295..16486)
                     /gene="hemP"
                     /locus_tag="ACG0BW_002187"
     CDS             complement(16295..16486)
                     /gene="hemP"
                     /locus_tag="ACG0BW_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFK7259141.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16618..17664)
                     /gene="aroH"
                     /locus_tag="ACG0BW_002188"
     CDS             complement(16618..17664)
                     /gene="aroH"
                     /locus_tag="ACG0BW_002188"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFK7259142.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17821..18654)
                     /gene="ppsR"
                     /locus_tag="ACG0BW_002189"
     CDS             complement(17821..18654)
                     /gene="ppsR"
                     /locus_tag="ACG0BW_002189"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFK7259143.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILESEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            18987..21365
                     /gene="ppsA"
                     /locus_tag="ACG0BW_002190"
     CDS             18987..21365
                     /gene="ppsA"
                     /locus_tag="ACG0BW_002190"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFK7259144.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQIMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(21422..23068)
                     /gene="fadK"
                     /locus_tag="ACG0BW_002191"
     CDS             complement(21422..23068)
                     /gene="fadK"
                     /locus_tag="ACG0BW_002191"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFK7259145.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQLVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCCMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(23125..23418)
                     /gene="ydiT"
                     /locus_tag="ACG0BW_002192"
     CDS             complement(23125..23418)
                     /gene="ydiT"
                     /locus_tag="ACG0BW_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFK7259146.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(23415..24704)
                     /gene="ydiS"
                     /locus_tag="ACG0BW_002193"
     CDS             complement(23415..24704)
                     /gene="ydiS"
                     /locus_tag="ACG0BW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFK7259147.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDVLEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQATATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(24760..25698)
                     /gene="ydiR"
                     /locus_tag="ACG0BW_002194"
     CDS             complement(24760..25698)
                     /gene="ydiR"
                     /locus_tag="ACG0BW_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFK7259148.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTAH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(25718..26482)
                     /gene="ydiQ"
                     /locus_tag="ACG0BW_002195"
     CDS             complement(25718..26482)
                     /gene="ydiQ"
                     /locus_tag="ACG0BW_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFK7259149.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQIATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPINQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEYLKKALN"
     gene            26798..>27293
                     /locus_tag="ACG0BW_002196"
     CDS             26798..>27293
                     /locus_tag="ACG0BW_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFK7259150.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENF"
BASE COUNT         6809 a         6509 c         7061 g         6914 t
ORIGIN      
        1 atttgttttc gccacatgct tattcaaaaa atgaaataag aatacattgc taagttattc
       61 gcgccttttt ataattcgtt acctttccag ggacgctaat ctttgatcct taagaaagtt
      121 tccgggaccg cgaaggtctc tttttttgtt gtacggactc ttgataaatg aagcttttga
      181 agacagttcc tgccatcgtt atgttggcgg ggggcatgtt tgcgtcactg aatgcagctg
      241 ccgatgattc cgtttttact gtcatggacg atcctgccag cgctaaaaaa cctttcgaag
      301 gtaatctgaa cgcaggatat cttgcacaat caggcaatac gaaaagctcc tcacttacgg
      361 ctgacaccac catgacctgg tatggacaaa ccactgcctg gtcgctgtgg gggaacgcca
      421 gtaatacttc ttctaatgat gagcgttctt cagaaaaata tgcggcgggc gggcgtagcc
      481 gttttaattt aactgattat gactatttat ttgggcaggc aagctggctg acagaccgtt
      541 ataacggcta tcgagagcgc gatgtgttga ccgcgggtta tggtcgccag tttttgaatg
      601 gcccggtaca cagtttccgc tttgaattcg gtccaggtgt gcgttacgac aaatataccg
      661 ataatgccag tgaaacccag ccgctgggtt atgcctccgg ggcttatgca tggcagttga
      721 ctgacaacgc taaatttacg cagggtgttt ccgtctttgg cgcggaagac acaacgctga
      781 actccgaaag cgcattaaat gttgctatta atgaacattt tggactgaag gtggcttaca
      841 acgtcacatg gaactctgaa cctccagagt ctgcaccgga acataccgat cgtcggacaa
      901 ccttatcgct gggctacagc atgtaaaatt atctgccgga ccgtgttatg cggtctggct
      961 gtcttcgttt gtcactgctc agccccaaat cattgctgcc caacgtagca acaacatagc
     1021 gccacagatt gccagtagca ccaccaccat cttccagtgt tttttatcaa agcgtttagt
     1081 cggcatacca gtgtcctgca tatctcttgt gcgtgataac ggagtgtaac aaaagggaca
     1141 ggcatcaagg gattttttgt tattacagcc gtcgggtggc gttgtacacc tgatcactgt
     1201 ttttgcggtt tatgaaaaaa ataaaattat gtatcgatat ataaatgtct ggttataaat
     1261 acgtctttag ttcatcagca ctaaaactca ggctttggct gattacgttt atagcagcat
     1321 aatatgtttc tacagtttta taatgattat gatgaatggc tatatgaaca gctgacatgt
     1381 tgtcatgatt tttagcggcc aacaatgtga ataactgaca ttgactaaaa gaatattttt
     1441 ttgcaaaagt acccagcgtt gatatataag ataacacgac gtctttatta cccttgctca
     1501 tggcgatgta taaagcaggg gttccatgtg ctgttgcgcc ttttaataaa tccatgatgt
     1561 taattttgct gaggttatat ttaacagcga ttccataaac tttagagagg aaccgtgtga
     1621 cacacaaagg gtgattattt tccattgcgg catataatcc tggctcacca tcacgtgttt
     1681 tcattgctaa aatatccatc accatttttt tgcttaaaag ctcttgtgtt attggagtgg
     1741 ttaatgcatt cagaaagata tcgattattt catcgaatcc atacaacatg gagatataga
     1801 gtccaggtgt ttcgttagca cttttagttt gcagtaattt aaccagatta tcttcatgaa
     1861 tgatatgatt atctaccagt gactgaattt cttgcataaa tatttttacg ttgtcagtat
     1921 gtccgttcat catagccaga aataaagcag gtgtaccaac gctattctgc gcagttatta
     1981 tttcaatgat ttctttttca gggagtatgg gtaactgttt gaataatgta ataagctgtt
     2041 gagtctggcc tgtggccatt attaacaaca ttgccgatgg atgtataggg gcaaatggca
     2101 ttcgtgatgc aatacccgcg aaaatagccc ctggaactcc ctcaggtaat aggggcagaa
     2161 aagcaattgc acagttgcga acatacctat cagcccaggt ctgagcatcg ggacctgaac
     2221 tgatgaactt atcgagcgag agaaagcccc gtgctgtcct gtgggtgcct ctgacggcaa
     2281 cgttggtatc gttcgggtcg taaacggaca caacataatt taattgccca tacgctgact
     2341 gctttattcg tagccttact gacaaagtat gattgtctat atataaaatc gcggacatat
     2401 gactgacatt gtttgccatc attcgacaga agaaaatatc taaagcgaag ccaaaatcct
     2461 tactcgcgat aacgcgactc cctgcaggag gatggcgaag taatgatctg aactccgcgt
     2521 cccggactaa gggaacatgc tgctgtatgg attcaacgga ggagtaatgt ttaatcagat
     2581 tgactttgcc ttttccattt ctcagtgaat caagtgcgta ttgcaatgac agatggcgac
     2641 aaaaaatagc gtcagaagga gattgcgaaa aaaatgcact accattgaga tttactgttg
     2701 gttgtatagt tttttctcta tggatttttt tagacagttt catacaatgg ttacgatttt
     2761 cggcagttaa ctgttcctgt tcaatataat ccagtatgct tcgcaaatgg ttagctttaa
     2821 taggcgtggt ttccagtaat aaggaaatga tttctttttt atagtgtaat gtaatgagta
     2881 tattttctgc atggatgggt gaagtggcaa agttaatggt aaaaagggca gtggaataac
     2941 aattttgtga tatttttcga cagtttgcgg taaattcctg agtaacacag tatgacagca
     3001 tcgaacttaa gtcagaggta tcaagtaggg tgatgagttg gcgcgcaaat tgattaatag
     3061 gctctgatga aggcgttggt atgtcgctat cgatgtgcat tctatgagga gacttataat
     3121 gagttctgat aataatgtcg ttttgactca taatctattc gccttatata tgtctggagg
     3181 ttttgaggat gatgattaat tattatcgcc agaacgaaat gtgcacgaaa aattgctata
     3241 agaattgtgt cacttttatc attgtgatat gaccacttcg tcattaatct tatgatcgtt
     3301 taaatagtac cgccaaatca cgcaacgaac tttgctggat tcagtttatg ctgctgcaaa
     3361 tccgctcgca gtaaaccttt cagacgctcc gtgatgttat cagtggttct ttaagcgttt
     3421 tgctggtgta ctcactacaa acgaattgcg aatcgaatca atgtgaaacg gaaaggtaca
     3481 atctcccttt ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag gttaatgtct
     3541 ttcatttgtg tacctgattg cgaaccaatt tagcatttgt tggctagatg gtttcgcaat
     3601 gaactgttaa taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc accactgcaa
     3661 ataaggatat aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca
     3721 cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat
     3781 cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca
     3841 actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc
     3901 gcacctgtta gggcacgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc
     3961 ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga
     4021 tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa
     4081 gaagaaagtc agctggcacg aagcgcgtga aactttcgcc aaccgtgggg agagctacaa
     4141 agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga
     4201 agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt
     4261 caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca
     4321 acgtatttac ggtacggcgt gggcagacaa aaaagcgctt aacgcttacc tgcagcgcct
     4381 ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat
     4441 gcaggaagaa gcaccgggta tggtcttctg gcacaacgat ggctggacca tcttccgtga
     4501 actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc
     4561 gttcatgatg gaccgtgttc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc
     4621 aatgttcacc acgtcttctg agaaccgtga atactgcatt aagccgatga actgcccggg
     4681 tcacgtacaa attttcaacc aggggctgaa gtcttatcgc gatctgccgc tgcgtatggc
     4741 cgagtttggt agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgtgt
     4801 acgtggtttt acccaggatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga
     4861 agttaacgga tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat
     4921 cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg
     4981 tgctgaggcg gacctggcgg ttgcgctgga agaaaacaac atcccgtttg aatatcaact
     5041 gggtgaaggc gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg
     5101 tgcatggcag tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc
     5161 ctatgtgggc gaagacaacg agcgtaaggt accggttatg attcaccgcg caattctggg
     5221 gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg
     5281 gcttgcgccg gttcaggttg ttatcatgaa tattaccgat tcacagtctg aatacgttaa
     5341 cgaattgacg caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga
     5401 gaagattggc tttaaaatcc gcgagcacac tttgcgtcgc gtcccgtata tgctggtctg
     5461 tggtgataaa gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct
     5521 gggaagcatg gacgtaaatg aagtgatcga gaagctgcaa caagagattc gcagccgcag
     5581 tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc
     5641 taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga
     5701 gcagcttggt attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga
     5761 cttagtcgag atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa
     5821 attcctctat gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca
     5881 ggttaaggaa attaaattcc gtcctggtac agatgaaggc gactatcagg taaaactccg
     5941 cagcctgatt cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgtgg
     6001 tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt
     6061 gcaagaactg gcagtggtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat
     6121 ggtgctcgct cctaagaaga aacagtaagg ccttcaagta gcactgtctg tggagccttt
     6181 gggcttcaca ggcattgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat
     6241 taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa
     6301 aggtggtttt aagcacaagc acgctaacct gcgtcacatt ctgaccaaaa aagcgaccaa
     6361 acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat
     6421 cgcgtgcctg ccgtacgcat aagccgttaa cgtttttaac tttttaatta gaatatagat
     6481 acaggagagc acatatggct cgcgtaaaac gtggtgttat tgcacgcgca cgtcacaaga
     6541 aaattttgaa acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac cgcgttgcct
     6601 tccaggctgt tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa cgtaagcgtc
     6661 agttccgtca actgtggatt gcgcgtatca acgcagcagc acgtcagaac ggtatttctt
     6721 acagcaaatt catcaatggc ctgaaaaaag cctctgttga aatcgaccgt aagatcctgg
     6781 ctgatatcgc agtattcgac aaagtagcgt tcaccgctct ggttgaaaaa gcgaaagcag
     6841 ctctggcata agccagttga aagagggagc tagtctccct cttttcgttt caacgccatc
     6901 aaaacattga cttttatcgc cgtagccttt tcaataaagg tcttttgaaa agtaccaaaa
     6961 ggtaacgcaa gcaatgaatg ctgctatttt ccgcttcttt ttttacttta gctcctgact
     7021 ccaggaggct agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc tcccagtgga
     7081 ggcttttttt gtatgcgcgt tttgaaaaaa ttaagtccaa cgaaccagtg tcaccactga
     7141 cacaatgagg aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat
     7201 tagccaggcg tcagatgttg ccgcgttaga caatgtgcgc gtcgaatatt tgggtaaaaa
     7261 agggcactta acccttcaga tgacgaccct gcgtgagctg ccgccagaag aacgcccggc
     7321 agccggtgcg gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcacgtaa
     7381 agcggaactg gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc
     7441 cctgccgggg cgtcgtattg aaaacggcgg tctgcatccg gtgacccgta ccatcgaccg
     7501 tatcgaaagt ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga
     7561 cgattatcat aacttcgatg ctctgaacat tcctggtcac cacccggcgc gtgctgacca
     7621 cgacactttc tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtgcagat
     7681 ccgcaccatg aaagctcagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg
     7741 taacgactac gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga
     7801 taccaacatc agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt
     7861 tgaggaagat ttgcagatcc gcttccgtcc ttcctacttc ccgtttaccg aaccttctgc
     7921 agaagtggac gtcatgggta aaaacggtaa atggctggaa gtactgggct gcggaatggt
     7981 acatccgaac gtactgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt
     8041 cgggatgggg atggagcgtc tgaccatgtt gcgttacggc gtcaccgacc tgcgttcatt
     8101 cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa
     8161 ttcagtgaac tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggca
     8221 aatcaaatca ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc
     8281 cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg
     8341 cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca
     8401 aactgccgtc agggcctgcg tgtggcggta gcgaccattg gtgctgtttt gccgggcgat
     8461 ttcaaaatta aagcggcgaa acttcgtggc gaaccgtctg aagggatgct gtgctccttt
     8521 tctgagctgg gaatttctga cgatcataac ggcataatcg agctgcctgc agatgcgccg
     8581 attggcaccg acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgtg
     8641 acaccaaacc gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg
     8701 aaccagctgc cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg
     8761 ctgccgatta cagtcgaagc gccggaagcc tgcccgcgtt atcttggccg tgtggtaaaa
     8821 ggcattaatg ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg
     8881 atccgttcta tcgatgcagt tgttgacgtc accaactatg tgctgctcga actgggccag
     8941 ccgatgcacg ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcgaaa
     9001 gagggcgaaa cgctggtact gctcgacggt accgaagcga agctgaacgc tgacaccctg
     9061 gtcatcgccg accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacattct
     9121 ggcgtaaatg aagaaacgca aaacgtcctg ttggaatgtg ctttcttcag cccactatct
     9181 atcaccggtc gtgctcgtcg tcatggcctg cataccgatg cgtctcaccg ttatgagcgt
     9241 ggcgttgatc cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac
     9301 atctgtggtg gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaaa
     9361 cgtgcaacca tcactttgcg tcgcagcaaa ctggatcgcc tgattggcca tcatattgcc
     9421 gatgagcagg tcactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac
     9481 gagtggcagg cagttgcgcc gagctggcgt ttcgacatgg agattgaaga agatctggtc
     9541 gaagaagtcg cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc
     9601 ctgattatgg gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc
     9661 aacgacaaag gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag
     9721 atgatccatc caggcgttga agccttactg ctgccaagcc ctatttccgt tgaaatgtca
     9781 gcaatgcgtc tttccctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt
     9841 cagcagaacc gtgtgcgcat tttcgaaagt ggtctgcgtt tcgtaccaga tactcaggca
     9901 ccgttgggca ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa
     9961 gagcactgga acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa
    10021 tccgttctcg acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg
    10081 gcactgcatc cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt
    10141 ggggttgttc atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc
    10201 gaactggagt ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga aatttctcgc
    10261 tttccggcga accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat
    10321 attttatccg aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac
    10381 gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg
    10441 caagatacca gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta
    10501 gaggcattaa aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa
    10561 agctgaaatg tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga
    10621 actggttgaa ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa
    10681 actctctggt tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc
    10741 gaaaacgggc gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca
    10801 gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa
    10861 aaaggccgct ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc
    10921 catgacactt cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa
    10981 atattcgctg gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt
    11041 gcgccggtga acaatggatt tcgcccggtg actggtctac tcctcgtggc gaactgttcg
    11101 tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta
    11161 tatccggcgc agtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg
    11221 gcgtctctaa cggcgcaggc gtagggctta tcgccgcggt gttgcttggg caagggcaac
    11281 tcccaaactg ggcactaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac
    11341 tcttacgttt cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat
    11401 tagggattat ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt
    11461 tacgtcagct gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct
    11521 ggctgatgct ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga
    11581 atatgttagc acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc
    11641 gcaatgtgct ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg
    11701 ctatcggctt tattggtctg gtgatccccc atattctccg gttatgtggt ttaaccgatc
    11761 atcgcgtatt acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggctgatg
    11821 ttgtagcgcg cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt
    11881 tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg
    11941 gtctatgatt aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc
    12001 cattctgacg accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc
    12061 cggtaatgtg ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga
    12121 gcagttggag aatattcaga aagcctgggc cgatcgtggt tttgtggtgc tgggattccc
    12181 gtgcaaccag tttctggagc aagaaccggg cagcgatgaa gagattaaaa cttactgcac
    12241 cactacatgg ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg
    12301 ccatccgctg tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag
    12361 cggattctat gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt
    12421 atggaatttt gagaaatttt tagttggcag ggacggattg gtaatccagc gtttttcccc
    12481 ggatatgacg ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcgaaata
    12541 atgtctattg tgatgcagct acaggatgtt gcggaatcaa cccgccttgg gccgctttct
    12601 ggcgaggttc gggcagggga gatcctgcat ctggtggggc cgaatggcgc gggtaagagt
    12661 accttactgg cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg
    12721 caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa
    12781 cagcagacgc cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat
    12841 aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc
    12901 ggacggagca ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg
    12961 gtgttgcaaa tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg
    13021 atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt
    13081 cagcaaggac tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg
    13141 catcgggcat ggttgctaaa aggtggaaaa atgctggcca gtggacgcag ggaagaggtg
    13201 ctcacgccgc caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa
    13261 ggtcaccgaa tgctgatttc gaccatttga tttcattagt atcttgaaaa tcgggtaatt
    13321 acgacgctaa attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc
    13381 ttattttgat cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa
    13441 atgccagact ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacagagct
    13501 ggcatggcac gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat
    13561 ttgtggttgt gacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac
    13621 aagcctctat cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt
    13681 tcaaaacggg ctccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat
    13741 ttatccacgc ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc
    13801 agaaaaattt ctggcaggcg agacgaatct agtatgtcga aaaatggacg gcgatgatac
    13861 atcgccgtcc atttactcca gaatacagta gtattttgca gacaaaaatt ccgtaaatgg
    13921 attattcgtt tataatcagg ataatctaaa aaaaggaccc ctgaaaaagt ggttaacatg
    13981 gaacaaatga aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc
    14041 cggatactga aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc
    14101 tttatcattc tgattgctat ttcctgccga taagagataa ccagcaagac cttgtcggcg
    14161 ttgagttaat tacccatttc tccagtgaag atggtacggt tcgtatcccg acgagtcggg
    14221 ttatagcgca acttaccgcg gagcaacatt ggcaattatt ttcagagcag ttggaattac
    14281 taaaatcatg ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc
    14341 cacaagtggc tactttatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc
    14401 cgtttattga actgttgatt aatgagaatt acccgcatct cgatgaagga aaagataatc
    14461 gcgatctttt gtccttatcg caaatgtatc cccttgtgtt aggtaatttg ggcgcaggca
    14521 atagcacaat gaaagcggtt tttgatggtc ttttcacccg cgtcatgctg gataaaagtt
    14581 ttattcagca acaaatcacg caccgctcct tcgaaccgtt tattcgtgcg atccaggcgc
    14641 aaatttcccc atgttgcaat tgcatcatcg cggggggcat tgatacgcca gaaataatgg
    14701 cacagattac ccccttcgat ttccatgctc tccagggatg cctgtggcct gccgttccca
    14761 taaatcagat aacgacgttg gttcagcgat aaccctcctg tttgctggtg tttaagacga
    14821 gagtaaccgt ctacactatc aaacaggagg atctatgacc ctgtcttttg ttacccgctg
    14881 gcgcgatgaa ttgccaggaa cctatacagc actttcccct acgcctttaa ataatgcccg
    14941 gctgatttgg cataataccg aactcgctaa cacgctgagt attccatcgt cgctgtttaa
    15001 aaatggcgca ggtgtctggg gcggcgaaac cctactgcct ggtatgtcac cactggcgca
    15061 ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat
    15121 tttactcggc gaacaacggc ttgctgatgg cactacaatg gactggcatc tgaaaggtgc
    15181 tggcctgacg ccttattcgc gaatgggtga tggacgggcg gttttacgtt cgacgatacg
    15241 agaaagtctc gccagtgagg cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag
    15301 tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat
    15361 gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa catttttact atcgccgcga
    15421 gccggaaaag gttcgtcagt tggctgactt tgccattcgt cattactggt cacatcttga
    15481 agatgatgag gacaaatacc gtctctggtt tagcgatgtt gtcgcacgta ccgcatcgct
    15541 aattgcccaa tggcagacgg tcggctttgc tcatggggtg atgaataccg acaacatgtc
    15601 gctgctgggg ctgacgcttg attacgggcc gtttggtttt cttgatgatt acgaacccgg
    15661 ttttatttgt aatcactcgg atcatcaagg gcgttatagc tttgataatc aacctgctgt
    15721 cgcgttgtgg aatttacagc gtctggcgca gacattgtca ccatttgttg ccgtagatgc
    15781 cctgaatgag gccctggaca gctatcagca ggttttgttg acgcattatg gacaacggat
    15841 gcggcagaaa ctgggcttca tgacggagca aaaagaggat aacgcgctac tgaatgaatt
    15901 attcagtctg atggcgcgag agcgcagcga ttatacccgc acattccgca tgctgagtct
    15961 gaccgagcag tacagcgcgg cgtcaccgct acgtgatgag tttatcgatc gtgcggcatt
    16021 tgatgactgg tttgcccgtt atcgggtacg tttgcaacaa gacgaggtta ccgatagtga
    16081 gcgtcagcag ctgatgcaaa gcgttaaccc agctctggtg ttgcgcaact ggttggcgca
    16141 acgggcgatt gaagcggcag aaaagggtga tatgacggaa ttgcaccgcc tgcatgaggc
    16201 gttgcgaaat cctttcagcg acagagatga tgactacgtc agtcgtccac ctgactgggg
    16261 taaacggctg gaagtcagct gctcgagtta aaggctactt ggtcaacagc agcttgccag
    16321 cctgagtttt acggagcaga tattcttgcc cgtcatgatc gataatcagt ttgccatccg
    16381 gacctaacag tgtctggctg gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat
    16441 tggcatccgt ttcaggcgtg agttttctgc tatccgtata acgcataaaa attaataata
    16501 attgatataa caatgattct tattatcaat attatagcgg gccagagcaa gcagtaatgt
    16561 gttgagaaga caacaagttg cgcagaaatg cccattccgt caggaatggg cacgcattca
    16621 gaagcgggta tctaccgcag aggcgagttt ttcgaccagg cgttcggtat cttcccagcc
    16681 cagacacggg tcggtaatcg attgaccgta agtgagcggc tgaccgccga cgattttttg
    16741 cgttccttcg cgcaggaaac tttccgccat aattccagca atcgccgtag agccattgcg
    16801 gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct tctggcagtt
    16861 accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca gcgtatcgca
    16921 ggctgcggcg atatcatcgg catggtaatt cggttttttg ccgccacgca taataatgtg
    16981 gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt ctggtgagag
    17041 gaacatatgg ctggcgcggg cagcgcggat agcatccaca gcaatccgcg tgttgccatc
    17101 ggtaccattt ttaaaaccta ccggacagga gagtgccgaa gccatttcac ggtggatctg
    17161 actttcggta gtacgtgcgc caatcgcacc ccaactgatt aaatcagcaa taaactgacc
    17221 ggtcaccata tcgaggaact cagtcgcggt agggacgccc agctcattta cctgtaaaag
    17281 taatttgcgc gccagctcca gaccgtgatt tacccgatag ctgccgttta aatccggatc
    17341 ggagattagt cctttccagc cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac
    17401 gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg cgtactccat
    17461 tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc gcttatcttc
    17521 accattcagt attttttcaa ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg
    17581 cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa tacgcgcagt
    17641 acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc tacggcagga
    17701 ctaccgggaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg
    17761 tgtcacacct cgccgggtgg ttagcatgat aacaaaaaaa taagctaatg cactagttct
    17821 ctagtacatt cggcggctaa ggcccatgat atcgaggatc ttggtggcaa tctcttctac
    17881 cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaagg cttctacttc
    17941 cgcgacttcc atcctgcact gacgaagcga ggcatagcga ctgttctccc gacgttcttc
    18001 gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat gctgaagcgg
    18061 tttgagcgac gcgggtagca ccagattatc catatcgtcg gcaataaagg ggtagtttgc
    18121 cgcgcggata ccaaactgca ttgccagata cagactggtg ggggttttac cacagcgaga
    18181 aacgccgagc aggatcacct gagcctggtc cagattgcgc aacgaaatgc cgtcatcgtg
    18241 ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat tattagggtt
    18301 aaggccgtgg gttcggtgag caatcggcgt tggatccagt ttcatctctt gttgtagcgg
    18361 ggcaaccagc gcctgaacga tatcctggca aaagccctca ctttccaaga tgatggcgcg
    18421 aatctccggc aacacgatgg agtagaagac cagcgggcgc acacctgtct ggtgataaat
    18481 tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa acggcagcgt
    18541 aatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata cctccgcagt
    18601 tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat ccattttgtt
    18661 cttcccgtga tgcagacata attctatgaa agcataaatt aaaaacgcac agaagcgtag
    18721 aatgttatgt ctggtttata aaatgaacct tcaattttat tatttatgaa aacagcattt
    18781 catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat agagcaggtt
    18841 tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga acgattcacc
    18901 gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt tctcaaaccg
    18961 ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg ctggtgcttt
    19021 ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat gcctccctgg
    19081 gtgaaatgat tactaacctt tccggaatgg gtgtttccgt tccgaatggt tttgccacaa
    19141 ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc atttatgaac
    19201 tgctggataa aacggatatt gacgatgtta cccagcttgc gaaagcgggc gcgcaaatcc
    19261 gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccatc cgcgaagcct
    19321 atgcacagct ttccgccgat gacgaaaacg cctcgtttgc ggtgcgttcc tccgccaccg
    19381 cagaagatat gccggacgct tcttttgccg gtcagcagga aactttcctc aacgttcagg
    19441 gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt aacgatcgcg
    19501 ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc tccgccggtg
    19561 ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc attgataccg
    19621 aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggt gaaatggtcg
    19681 tgcagggtgc ggttaacccg gatgagtttt acgtgcacaa accgacactg gcggcgaatc
    19741 gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt tacgcgccga
    19801 cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag cgtgacatct
    19861 tctcgctgac caacgaagaa gtgcaggaac ttgcaaaaca ggccgtacaa attgagaaac
    19921 actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggt aaactgttca
    19981 ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca gatcatggag cgttatacgc
    20041 tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc atcggcgcgg
    20101 gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct ggcgacgtgc
    20161 tggttactga catgaccgac ccggactggg aaccgatcat gaagaaggca tctgccatcg
    20221 tcaccaatcg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa ctcggcattc
    20281 cggcggtagt gggctgtggt gatgcaacag aacggatgaa agacggtgag aacgtcactg
    20341 tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa tttagcgtga
    20401 aaagctccag cgtagaaacg atgcctgatc tgccgttgaa ggtgatgatg aacgtcggta
    20461 acccggaccg tgctttcgac ttcgcctgcc tgccgaacga aggcgtgggc cttgcgcgtc
    20521 tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt gagtttgacg
    20581 atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt gattctccgc
    20641 gtgaatttta cgttgggcgt ctgactgaag ggatcgcgac gctgggtgcc gcgttttatc
    20701 cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc aacctggtcg
    20761 gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt ggcgcgggcc
    20821 gctatgtttc cgacagcttc cgcgactgct ttgcgctgga gtgtgaagca gtgaaacgtg
    20881 tgcgcaacga catgggactg accaacgttg agatcatgat cccgttcgtg cgtaccgtag
    20941 atcaggcgaa agcggtggtt gacgaattgg cgcgtcaggg gctgaaacgt ggtgagaacg
    21001 ggctgaaaat catcatgatg tgtgaaattc cgtccaacgc cttgctggca gagcagttcc
    21061 tcgaatattt cgacggcttc tcaattggct caaacgacat gacacagctg gcgctcggtc
    21121 tggaccgtga ctccggagtg gtgtctgaac tgttcgatga gcgcaacgat gcggtgaaag
    21181 cactgctgtc gatggcgatt cgtgccgcga agaaacaggg caaatatgtc gggatttgcg
    21241 gtcagggccc gtccgaccac gaagactttg ccgcatggtt gatggaagaa gggatcgata
    21301 gcctgtctct gaacccggat accgtggtgc aaacctggtt aagcctggct gaactgaaga
    21361 aataaaataa atccccggcg gcgttcagtc gccgggttta tgtgatcccc gaagatgaaa
    21421 cttattcaat ctcttcacag acatcctgag ttaatctccg cataatatct ttccttaaca
    21481 aaaacttttg tattttacct gaggcagtgc gcggtagttt ttcgattacc acgatatgtt
    21541 caggatattt atatttcgcg acccgtttac ggctaaaaaa agccaccacc tcttccagcg
    21601 ataatgaatg atgcggtgct ttcagcacga cataagcgca tgagcgttca cctaaacgtt
    21661 catcgggcat cgcaaccaca caggcatcgt gaattttagg atgctgcaat aaaatatctt
    21721 ctacttcacg gctgctaata ttttcgccgc cgcggacaat aatatctttc ttgcgcccgg
    21781 ttatttttat atagccggcc tcatccatgc agcagagatc gccgctgtaa taccagcctt
    21841 cttcatccag ggcacgggcg gttaattcag gttcatcaaa ataccccata aacacattgg
    21901 ggccacgcga ggcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt gcgtcatcga
    21961 ccactttaat ctcaacacct gcggcagcgt aaccatcggt gtgcataaag cgcgacaaag
    22021 gatcgtcgag attcaccact gcgtgcggcg aactttctgt ggaaccataa acacttaata
    22081 atttaatgcc gcgctgctgg cattcacgcg ccactttttt ggggattgtg gtcccgccgc
    22141 aaagaaagaa gcgcagcgct gaaaggtcag cgggttgttt ctccagtaaa ttcaaaagat
    22201 catagacaaa cggcgttgcg ccgagcatac aggtgcaacg ctgctgctca agcagcgcga
    22261 gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt aagaatggtg
    22321 ccgttacgcc atgcagaaag cccgttgcat gaccaagtgg cgcaggcatc ataaagacat
    22381 cctgccaggt cagattcaac cgcgcgcaat aagcccgctc actggcgaga atattgttat
    22441 gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc accgcggcta
    22501 attcatcgcc gtggaccgtt atcgccgtgg ttagaggagt attgtcggcg ataatctgac
    22561 ttaatgagag agcagaggtg gcgggagcca gtttgtccac gccgacaagt tgttgtagtt
    22621 gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt ttaaacaacg
    22681 tcggagcaaa gaacattttt gcctgacact tattgagcac ccatactagt tctgcttccc
    22741 gccaggaagg caacagcggt acggaaaccg caccgatttt caggcaggca agatagataa
    22801 cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat tcaataccct
    22861 tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta taggtgtacg
    22921 atgctccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg gtctgctgcc
    22981 agtaatcggc cagcgaagca tcgccccata gcccttgctg acgatacgcc gcacgacgtt
    23041 gttcgttaaa cgttaatgtc actttcattt tggactctcg agacagaaca tcaggcccga
    23101 gatgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga aggtgcctgc
    23161 gggatattgc cactgttcga gaatagtgtt gccgcacagc acccgacagg tgccgcactc
    23221 cagacaaccg gcagaatcaa aatgaatgtt gccggcgtca tcctgcttat aaagtccggc
    23281 agggcaggct ttcattaatt tacggaattc attgatatcg ggatttgccg ccaaaatgat
    23341 atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt cgacgttaac
    23401 cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag caagttgatc
    23461 agcccaattt ttttcgcgtg tcccatgatc attttgcgta ccggctggtt tggtttgccg
    23521 tcaatggtga acatatcgtt catgatgtcg gcgaccatgc gcggatactg actaaacagg
    23581 cgcggatttt ccatcagcgc cgggatcttg cggaaatgct gcatatcgcg cataacacaa
    23641 ctttgttcca gctcacgttt gtattgcgcc agactgctgg cggagaaatc cgcgcgttct
    23701 ttggcggcaa tcactgttgt ggcggtagcc tgagctgatg caatggctaa atccatgccg
    23761 cgaactgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat catcacgcca
    23821 tcgttaacca gctgcggcac cattgccaga ccgccttccg gcaccatatg cgcggaatat
    23881 tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt aaaatcttcc
    23941 agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca aaccagcccc
    24001 aacgatatgg aatccttatt ggtatagagg aatccgccac ccatcaggcc gtcagaaggg
    24061 gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg atcgttgatc
    24121 tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg cggatcggaa
    24181 gcggaaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc cagaatcacc
    24241 acattcgctt caagaacatc atccccggcc tgcacgccag tgaccttgtt tccttcacga
    24301 accagcgcat cgacgcgtac gcccgggata aactgcgcgc ccgcctgctc ggcttgttcc
    24361 atcaaccacg ggtccagacg attacgcaat acggtataag acgcgtgttg cggaacatct
    24421 ggttgctcgc ggtgaaagtc gagggtaacg gcgctttctt cggttaagaa ggagattttc
    24481 tcgcgtgtga ccttgcgttc taccggcgct gatgctgcaa agcctggaat aatcgcttca
    24541 agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact gtcgccgcgt
    24601 tctatcacca ggacatccag cccggctcgc gccatgacca gtgcggcaac gctaccggca
    24661 acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta tgctcccttc
    24721 ccgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca actggctaat
    24781 cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat agttgaagat
    24841 tggcgcattt ttatctttat tgatggcgac aatcactttt gcgccgttgc cgccaaccat
    24901 atgctggatc tgcccggaga tccccagcgt caggtagaga tcggatttca gcaacacgcc
    24961 ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa ttggacgtga
    25021 acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca ggtcatcctg
    25081 cgctgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt ccacgctgct
    25141 tgccgctttc gcacggcgtt cgcgacagag aatttcatga cgcggggcaa cataaggtac
    25201 cgtttctgtc gggcactgat gagcggtatc actggtgcac ggttcctgaa caccgggtgc
    25261 aagggtaatg atcgccagcg ggctgttgat cttttcctgt gcgaacgcta acccgccata
    25321 catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgcagtgg catcgttcac
    25381 cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccgc gtttcgtcgc
    25441 ggccagcagc aacatagaag gatgtttatc tttcagcagg gcagcaatgc tttcggcgta
    25501 attttcagta cgttgcagcg catcattttg ctcaagaaca taaatacatt ttggaccata
    25561 aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct gttggcccca
    25621 ttgctgcgcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa agacccagac
    25681 gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct ttcttcagat
    25741 attccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg tgcttacgtt
    25801 ctgtttgcgg cggtacgcga atgccggtca gctcagcaag tggcgcgctc tggctccagc
    25861 caatgtcact tgcctgccac tgatttatcg gttttttacc cgcgccgaga atggctttca
    25921 tcgaaggaat gcgtggcata ttaatatcgg aggtgacgca gagcacggct ggaacagaga
    25981 gctcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta ttgccctgac
    26041 gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg accagcaagc
    26101 caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa tcgaagccga
    26161 tcttttcaac tgctgccgcc agcgcctttg cggtatcgag aggcagtgca tgttcaagtt
    26221 gcgcatcctg caccaaatac aggctatgcg gcccgcggga tagcacgtct ttacgcactt
    26281 tcgagttctg caacaatgag ccgccaatgg tcagcgcggc tatctcatcg tcatctgttg
    26341 caatctggct tgcagcttca atggcattga gatcgaactg gctgattttg gcgtcggcat
    26401 tgtcgaaatt cagggtatat tctggagtga caacaatgtc ctgttcttca ggcaccagct
    26461 taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac catctccgat
    26521 ggaaggtaaa aatggtctga aaatagtgtg cgatagcggt gcggggtttt cgacggagca
    26581 agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tcgctaaaac aaaagtgcaa
    26641 tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta aataatcttg
    26701 tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt atccggcata
    26761 tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct gttttgataa
    26821 cgccagcgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt ttgcatttag
    26881 cgatgatcca aaatgggagt ccggacatca cgttcatgac aatgaaactg agttgattta
    26941 cgtcaagaaa ggggtagcaa ggtttaccat cgattcttcg ttatatgtcg cgcacgccga
    27001 tgacattgtg gtgatagaac gtggcaggct gcatgcggtg gcctctgacg ttaacgatcc
    27061 ggcaacgacg tgtacttgtg cgctgtacgg ctttcagttt cagggggctg aggaaaatca
    27121 gctactgcaa ccgcattctt gcccggtaat tgccgcaggg cagggaaaag aagtcattaa
    27181 aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc aaacatcttc
    27241 gttatgggac gcctttgcct atacgttagc aattctttac tacgaaaact tta
//
LOCUS       DAYPHN010000055        27218 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000055 DAYPHN010000000
VERSION     DAYPHN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.055"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            250..1509
                     /gene="codB"
                     /locus_tag="ACG0BW_002197"
     CDS             250..1509
                     /gene="codB"
                     /locus_tag="ACG0BW_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFK7259151.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            1499..2782
                     /gene="codA"
                     /locus_tag="ACG0BW_002198"
     CDS             1499..2782
                     /gene="codA"
                     /locus_tag="ACG0BW_002198"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFK7259152.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALSQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            2822..2913
                     /locus_tag="ACG0BW_002199"
                     /pseudo
     CDS             2822..2913
                     /locus_tag="ACG0BW_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2915..3814)
                     /gene="cynR"
                     /locus_tag="ACG0BW_002200"
     CDS             complement(2915..3814)
                     /gene="cynR"
                     /locus_tag="ACG0BW_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFK7259153.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLKLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHEGLKAISLAPPLLKRTAVLLRRKNSWQT
                     AAAKAFLQMALDKCAVVGGNESR"
     gene            3924..4583
                     /gene="cynT"
                     /locus_tag="ACG0BW_002201"
     CDS             3924..4583
                     /gene="cynT"
                     /locus_tag="ACG0BW_002201"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFK7259154.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHRDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4614..5084
                     /gene="cynS"
                     /locus_tag="ACG0BW_002202"
     CDS             4614..5084
                     /gene="cynS"
                     /locus_tag="ACG0BW_002202"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFK7259155.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5090..6271
                     /gene="cynX"
                     /locus_tag="ACG0BW_002203"
     CDS             5090..6271
                     /gene="cynX"
                     /locus_tag="ACG0BW_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFK7259156.1"
                     /translation="MRDHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6359..6970)
                     /gene="lacA"
                     /locus_tag="ACG0BW_002204"
     CDS             complement(6359..6970)
                     /gene="lacA"
                     /locus_tag="ACG0BW_002204"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFK7259157.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVEPSV"
     gene            complement(7036..8289)
                     /gene="lacY"
                     /locus_tag="ACG0BW_002205"
     CDS             complement(7036..8289)
                     /gene="lacY"
                     /locus_tag="ACG0BW_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFK7259158.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8341..11415)
                     /gene="lacZ"
                     /locus_tag="ACG0BW_002206"
     CDS             complement(8341..11415)
                     /gene="lacZ"
                     /locus_tag="ACG0BW_002206"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFK7259159.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTIVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPVENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVKLHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPVLPQPESTGQLWLTVHVVQPNATAWSEAGHISAWHQWRLAENLSVTLPAASHSIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRAQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11538..12620)
                     /gene="lacI"
                     /locus_tag="ACG0BW_002207"
     CDS             complement(11538..12620)
                     /gene="lacI"
                     /locus_tag="ACG0BW_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFK7259160.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEDIVPTAMLVANDQMALGAMRAITESGLHVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            complement(12697..13530)
                     /gene="mhpR"
                     /locus_tag="ACG0BW_002208"
     CDS             complement(12697..13530)
                     /gene="mhpR"
                     /locus_tag="ACG0BW_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFK7259161.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGMHL
                     R"
     gene            13721..15385
                     /gene="mhpA"
                     /locus_tag="ACG0BW_002209"
     CDS             13721..15385
                     /gene="mhpA"
                     /locus_tag="ACG0BW_002209"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFK7259162.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            15387..16331
                     /gene="mhpB"
                     /locus_tag="ACG0BW_002210"
     CDS             15387..16331
                     /gene="mhpB"
                     /locus_tag="ACG0BW_002210"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFK7259163.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            16349..17215
                     /gene="mhpC"
                     /locus_tag="ACG0BW_002211"
     CDS             16349..17215
                     /gene="mhpC"
                     /locus_tag="ACG0BW_002211"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFK7259164.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            17225..18034
                     /gene="mhpD"
                     /locus_tag="ACG0BW_002212"
     CDS             17225..18034
                     /gene="mhpD"
                     /locus_tag="ACG0BW_002212"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFK7259165.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIENAEAAYAIQRI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNVIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            18031..18981
                     /gene="mhpF"
                     /locus_tag="ACG0BW_002213"
     CDS             18031..18981
                     /gene="mhpF"
                     /locus_tag="ACG0BW_002213"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFK7259166.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            18978..19991
                     /gene="mhpE"
                     /locus_tag="ACG0BW_002214"
     CDS             18978..19991
                     /gene="mhpE"
                     /locus_tag="ACG0BW_002214"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFK7259167.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            20167..21378
                     /gene="mhpT"
                     /locus_tag="ACG0BW_002215"
     CDS             20167..21378
                     /gene="mhpT"
                     /locus_tag="ACG0BW_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFK7259168.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLITGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            21479..22018
                     /gene="yaiL"
                     /locus_tag="ACG0BW_002216"
     CDS             21479..22018
                     /gene="yaiL"
                     /locus_tag="ACG0BW_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFK7259169.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIVNGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLLDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(22143..22976)
                     /gene="fghA"
                     /locus_tag="ACG0BW_002217"
     CDS             complement(22143..22976)
                     /gene="fghA"
                     /locus_tag="ACG0BW_002217"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFK7259170.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFTAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(23068..24177)
                     /gene="frmA"
                     /locus_tag="ACG0BW_002218"
     CDS             complement(23068..24177)
                     /gene="frmA"
                     /locus_tag="ACG0BW_002218"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFK7259171.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(24212..24487)
                     /gene="frmR"
                     /locus_tag="ACG0BW_002219"
     CDS             complement(24212..24487)
                     /gene="frmR"
                     /locus_tag="ACG0BW_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFK7259172.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(24675..25448)
                     /gene="yaiO"
                     /locus_tag="ACG0BW_002220"
     CDS             complement(24675..25448)
                     /gene="yaiO"
                     /locus_tag="ACG0BW_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFK7259173.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVISSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(25450..26043)
                     /locus_tag="ACG0BW_002221"
     CDS             complement(25450..26043)
                     /locus_tag="ACG0BW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFK7259174.1"
                     /translation="MSQELKNFLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHG
                     AVIQGPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIAD
                     SVVANQAYLGAQVRTSNHRLDQQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIIL
                     PGRIISPNTQLGPRVIVERNLPPGTYSLRQELIRTGD"
     gene            complement(26009..27205)
                     /gene="yaiP"
                     /locus_tag="ACG0BW_002222"
     CDS             complement(26009..27205)
                     /gene="yaiP"
                     /locus_tag="ACG0BW_002222"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFK7259175.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLKRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPYGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTSYSQPSVTGT
                     EKLSEA"
BASE COUNT         6237 a         7090 c         7439 g         6452 t
ORIGIN      
        1 ctgaaccgta ggtcggataa ggcgctcgcg tcgcatccga caccccgcgc cgatgcctga
       61 tgcgacgctg acgcgtctta tcaggcctac ccactaccca ctgtttttac accgataatt
      121 tttcccccac ctttttgcac tcattcatat aaaaaatata tttccccacg aaaacgattg
      181 ctttttatct tcagatgaat agaatgcggc gaattttttg ggtttcaaac agcaaaaagg
      241 gggaatttcg tgtcgcaaga taacaacttt agccaggggc cagtcccgca gtcggcgcgg
      301 aaaggggtat tggcattgac gttcgtcatg ctgggattaa ccttcttttc cgccagtatg
      361 tggaccggcg gcactctcgg aaccggtctt agctatcatg atttcttcct cgcagttctc
      421 atcggtaatc ttctcctcgg tatttacact tcatttctcg gttacattgg cgcaaaaacc
      481 ggcctgacca ctcatcttct tgctcgcttc tcgtttggtg ttaaaggctc atggctgcct
      541 tcactgctac tgggcggaac tcaggttggc tggtttggcg tcggtgtggc gatgtttgct
      601 attccggtgg gtaaggcaac cgggctggat attaatttgc tgattgccgt ttccggttta
      661 ctgatgaccg tcaccgtctt ttttggcatt tcggcgctga cggttctttc ggtgattgcg
      721 gttccggcta tcgcctgcct gggcggttat tccgtgtggc tggctgttaa cggcatgggc
      781 ggcctggacg cattaaaagc ggtcgttccc gcacaaccgt tagatttcaa tgtcgcgctg
      841 gcgctggttg tggggtcatt tatcagtgcg ggtacgctca ccgctgactt tgtccggttt
      901 ggtcgcaatg ccaaactggc ggtgctggtg gcgatggtgg cctttttcct cggcaactcg
      961 ttgatgttta ttttcggtgc agcgggcgct gcggcactgg ggatggcgga tatctctgat
     1021 gtgatgattg ctcagggcct gctgctgcct gcgattgtgg tgctggggct gaatatctgg
     1081 accaccaacg ataacgcact ctatgcgtcg ggtttaggtt tcgccaacat tactggtatg
     1141 tcgagcaaaa ccctttcggt gattaacggt attatcggta cggtctgcgc attatggctg
     1201 tataacaatt ttgtcggctg gttgaccttc ctttcggcag ctattcctcc ggtaggtggc
     1261 gtgatcattg ccgactatct gatgaaccgt cgccgctatg agcactttgc gaccacgcgt
     1321 atgatgagtg tcaattgggt ggcgattctg gcggtcgcgc tggggattgc cgcaggccac
     1381 tggttaccgg gaattgttcc ggtcaacgcg gtattaggtg gcgcgctgag ctatctgatc
     1441 cttaacccga ttttgaatcg taaaacgaca gcagcaatga cgcatgtgga ggttaacagt
     1501 gtcgaataac gctttacaaa caattattaa cgcccggtta ccaggcaaag aggggctgtg
     1561 gcagattcat ctgcaggacg gaaaaatcag cgccattgat gcgcaatccg gcgtgatgcc
     1621 cataactgaa aacagcctgg atgccgaaca aggtttagtt ataccgccgt ttgtggagcc
     1681 gcatattcac ctggacacca cgcaaaccgc cggacaaccg aactggaatc agtccggcac
     1741 gctgtttgaa ggcattgaac gctgggccga gcgcaaagcg ttattaaccc atgacgatgt
     1801 gaaacaacgc gcctggcaaa cgctgaaatg gcagattgcc aacggcattc agcatgtgcg
     1861 tacccatgtc gatgtttcgg atgcaacgct aactgcgctg aaagcaatgc tggaagtgaa
     1921 gcaggaagtc gcgccgtgga ttgatctgca aattgtcgcc ttccctcagg aagggatttt
     1981 gtcgtatccc aacggtgaag cgttgctgga agaggcgtta cgcttagggg cagatgtagt
     2041 gggggcgatt ccgcattttg aatttacccg tgaatacggc gtggagtcgc tgcataaaac
     2101 cttcgccctg tcgcaaaaat acgaccgtct catcgacgtt cactgtgatg agattgatga
     2161 cgaacagtcg cgctttgtcg aaaccgttgc agccctggcg caccgtgaag gcatgggcgc
     2221 gcgagtcacc gccagccaca caacggcaat gcactcctat aacggggcgt atacctcacg
     2281 cctgttccgc ttgctgaaaa tgtccggtat taactttgtc gccaacccgc tggtcaatat
     2341 tcatctgcaa ggacgtttcg atacgtatcc aaaacgtcgc ggcatcacgc gcgttaaaga
     2401 gatgctggag tccggcatta acgtctgctt tggtcacgat gatgtcttcg atccgtggta
     2461 tccgctggga acggcgaata tgctgcaagt gctgcatatg gggctgcatg tttgccagct
     2521 gatgggctac gggcagatta acgacggtct gaatttaatc acccaccaca gcgccaggac
     2581 gttgaatttg caggattacg gcattgccgc cggaaacagc gccaacctga ttatcctgcc
     2641 tgcagaaaat gggtttgatg cgctgcgccg tcaggttccg gtgcgctatt cggtacgtgg
     2701 cggtaaggtg attgccagca cccaaccagc acaaaccacc gtatatctgg agcagccaga
     2761 agccatcgat tacaaacggt gatcggctgg gctgtgtgtt gacaaagcga gcgtagttta
     2821 tgccggatgc ggcgtgaacg ccttatccgg cctacgtaga gcactgaact cgtaggcctg
     2881 ataagcgtag cgcatcaggc aattgtgtca gtggttaccg cgattcattc ccgccaacaa
     2941 ccgcgcattt atccaacgcc atttgcaaaa atgccttcgc ggcggctgtc tgccagctat
     3001 ttttccgccg caacaaaacc gccgttctct tcagtagtgg tggggcaaga gaaatagctt
     3061 taagcccttc atgttgtgtg gcaatcgctg ctggcaacaa tgtggaaagg gaagtgcggc
     3121 gaatcagctc cagaaccgcg ctaattgagt tcgcctcaat gaccacctgt ggatgtagcc
     3181 ccgctttctc acagtagtgg tcaatttgct ctctggtggc aaattccgcg ctgagcagga
     3241 ccagtttttc atcatgcaag cgactcaacg ccacctgttc acaggcagcc agcggatgat
     3301 gttgcgccac gactaacgct aaactttctg tcagtaaagg aattgcctcc agctccggcg
     3361 aatgcacagg cgcgaaggca atcccaacgt ccaactcgtc gcgacaaagc agatcttcga
     3421 ttttctcctg cgacatttcc tgtagcttga gcgtgatgcc ggggtagcgc gcatagaaat
     3481 ccgccattaa agggccgata aagtagctcg taaaggtggg ggtgacggcg atacgcagcg
     3541 atcctcgcgt cagatcggca acatcatgaa tcgcccgttt acccgccccc agttcctgta
     3601 acgcccggct ggcgtactgt cgccagactt ctcctgcatc agtgagacga atcgttcgcc
     3661 cgctacggtc aaacagcggc acgcctaaac tctcctctaa ctggcgaatc tgctgggaaa
     3721 gcgcaggctg ggagacgtgc aacgcactgg cggcacgggt aaagctgcca tgttcagcca
     3781 cggcaagaaa ataattgata tgtcgagaga gcattcgcca acctataagt aaatccaata
     3841 gaactcatca taaatgagac ttttacctta tgacaatcgg cgagtagtct gcctctcatt
     3901 ccagagacag acagaggtta acggtgaaag agattattga tggattcctt aaattccagc
     3961 gcgaggcatt tccgaagcgg gaagccttgt ttaaacagct ggcgacacag caaagcccgc
     4021 gcacactttt tatctcctgc tccgacagcc gtctggtccc tgagctggtg acgcaacgtg
     4081 agcctggcga tctgttcgtt attcgcaacg cgggcaatat cgttccttcc tacgggccag
     4141 aacccggtgg cgtttctgct tcggtggagt acgccgtcgc tgcgcttcgg gtatctgaca
     4201 ttgtgatttg cggtcattcc aactgtggcg caatgaccgc tattgccagc tgccagtgca
     4261 tggaccatat gcctgccgtc tctcactggc tgcgttatgc cgattctgcc cgcgtcgtta
     4321 atgaggcgcg cccgcatcgt gatttaccgt caaaagctgc ggcgatggtg cgtgaaaacg
     4381 tgattgctca gttggctaat ctgcaaacgc atccatcggt gcgcctggcg ctcgaagagg
     4441 ggcggatcgc cctgcacggc tgggtctacg acattgaaag cggcagcatc gcagcttttg
     4501 acggcgcaac ccgccagttt gtgccactgg ccgctaatcc tcgcgtttgt gccataccgc
     4561 tacgccaacc gaccgcagcg taaccttatt tttaaaccat caggagatcc tccatgattc
     4621 agtcgcaaat taaccgcaat attcgtcttg atcttgccga tgccattttg ctcagcaaag
     4681 ctaaaaaaga tctctcattt gccgagattg ccgacggcac cggtctggca gaagccttcg
     4741 taaccgcggc tttgctgggt cagcaggcgc ttcctgccga cgccgcccgc caggtcgggg
     4801 cgaagctgga tctcgacgaa gacgctattc tactgttgca gatgattcca ctgcgtggct
     4861 gcattgatga ccgtattcca accgacccaa cgatgtatcg tttctatgaa atgttgcagg
     4921 tgtacggcac aaccctgaaa gcgttggttc atgagaagtt tggcgatggc attatcagcg
     4981 cgattaactt caaactcgac gttaagaaag tggcggatcc ggaaggtggc gaacgcgcgg
     5041 tcatcacctt agatggcaaa tatctgccga ccaaaccgtt ctgacaacca tgcgcgacca
     5101 tcaaaagacg ttcacgatgc tgctggttct ggtgctgatt ggtcttaata tgcgaccact
     5161 gctcacctcc gtcggaccac tgctaccgca attgcgtcag gcgagtggaa tgagctttag
     5221 cgtggcagcc ctgttgactg ctctgccggt ggttaccatg ggcgggctgg cgctggccgg
     5281 aagctggctt catcagcatg tcagcgaacg tcgcagcgtc gccatcagtt tgttactgat
     5341 tgccgtcggt gcattgatgc gtgagcttta cccccaaagt gcgctgctgc ttagcagcgc
     5401 actgcttggt ggagtgggga tcggcatcat tcaggcagtg atgccttcgg taattaagcg
     5461 gcggtttcag caccgtacgc cactggtgat ggggctgtgg tccgcggctc tgatgggcgg
     5521 cggtggactt ggtgccgcca ttacgccctg gttagttcaa catagcgaaa cctggtatca
     5581 aacactcgcc tggtgggcgc tgcctgccgt tgttgcgctc tttgcctggt ggtggcaaag
     5641 cgcccgcgag gtcgcctctt ctcacaagac aacaaccact ccggttcgcg tggtatttac
     5701 tccccgcgcc tggacgctgg gtgtttactt cggtctgatt aacggcggtt acgccagcct
     5761 gattgcctgg ttacccgctt tctatattga gattggtgcc agcgcgcagt acagcggttc
     5821 cttgctggca ttgatgacgc ttgggcaagc agcaggggca ttgctaatgc ctgctatggc
     5881 tcgccatcag gatcggcgca aacttttgat gctggcgctg gcgttacaac tggtggggtt
     5941 ctgcggcttt atctggctgc cgctgcaatt gccggtattg tgggcaatgg tgtgtgggtt
     6001 aggtctgggc ggcgcatttc cgctctgttt gctgctggcg ctcgatcact ctgcgcaacc
     6061 cgctattgct ggcaaactgg tggcgtttat gcaggggatc ggttttatca tcgccgggct
     6121 tgccccgtgg ttttctggtg tgctgcgtag tatcagtggc aattacttga tggactgggc
     6181 gtttcatgcg ctgtgcgtcg ttgggctgat gatcataacc ctgcgttttg caccagcacg
     6241 ttttccgcag ctgtgggtca aagaggcatg atgcgacgct tgtttcgcca gataatggaa
     6301 gagtgaatta ttgtcggatg tgcccaatgg cgcatccgac atttatttca gtgctaattt
     6361 aaactgaagg ttcaacttta taatctttgt aataatagtg cttatcccgg tcgtttattt
     6421 cgcgaataac ccggcaagga acgccagcag ccacgacgtt tggtggaatg tcttttatga
     6481 cgacactacc cgcgccaata acagaattat ccccgatggt gacgcctgga ttaatgacca
     6541 catgacttcc gatccagacg ttattgccaa tcgttatcgg aaaagagtac atctcaccgt
     6601 tttttcttaa ttcatggtgt accgggtgtc ccgtaacgga aagagtaacg ttgggtgcaa
     6661 tcagtacgtt atcaccgatt gttaccgtgt agtcatcgac aatggttaaa ttaaaatttg
     6721 cataaaaatt gcggcctata tggatgttgg aaccgtaaga gaaatagacg ggaggttcta
     6781 cccaggcgtt ttcccctacc gtggcaaaca tttctttaat caggctttct cttttttcaa
     6841 cttctgatgg atgcgagtga ttaaactcat acattaacgt tttcccacga agtctttttt
     6901 ccggtaagcc ttcgcacata tcggtaaata gcttgcctgc ttttattctt tcggtcattg
     6961 gcatgttcat tgcgatcatt ccgttatgat atgttggtcg gataaggcgc tcgcgccgca
     7021 tccgacattg attgcttaag cgacttcatt cacctgacga cgcagtagag aaagcggacc
     7081 ggggccgcta agcgtgaaca cggaaattaa ggtgaagccc aacgccacca gacccagcac
     7141 cagataagcg ccctggaaac cgatgctttc atacatattg cccgccagta cagacataaa
     7201 aatcatcgcc agttgcttaa agaagcagaa acagaccaga taaatcgtcg ctgaaaaacg
     7261 cacttcaaac tggctggtaa tatatttaaa gcagcccacc agcaggaacg gtacttcaaa
     7321 catatgcagc gttttcagaa taaccacttc cagcgctgag gtggcgaacg atgagccaat
     7381 aatacgtaca gacataatag tgccagccag cagcagggcg tttttcccac cgatgcgatt
     7441 aatgatcagc ggcgcgaaga acataatcga ggcgttaagt aattcgccca ttgtcgttac
     7501 gtagccaaat acccgcgtac cctgttcacc ggtggcaaag aacgaagtaa agaaattagc
     7561 aaactgttgg tcaaaaacat cgtaggtgca ggaaacgcca ataacataca gtgacaaaaa
     7621 ccacagtttt ggctgtctga acagttccag cgccagctta aggctaaatg ccgaatggtt
     7681 ggcacctacc gcattggcaa ccgtggcgga agagggtgca tccgttttgg cgaaaaagag
     7741 caaaacggcg aggatgagtg cacagccaga acccagccag aaaacaaact gattattgat
     7801 ggtgaacatg atgccgacaa tcgaggcaca cagcgcccag ccaacacagc caaacatccg
     7861 cgcgcgacca aattcgaaat tactgcgacg gctgactttc tcgataaatg cctctactgc
     7921 tggcgcaccg gcgttaaaac aaaagccaag ataaatacca ccaacaatcg atcctactaa
     7981 aatgttgtat tgtaacagtg gcccgaagat aaaaataaag aacggcgcaa acatcactaa
     8041 catgccggta ataatccaca gcaggtattt gcgcagcccg agtttgtcag aaagcagacc
     8101 aaacagcggt tggaataata gcgagaacag agaaatagca gcaaaaataa tacccgtatc
     8161 acttttgctg atatggttga tgtcatgtag ccaaatcggg aaaaacggga agtaggctcc
     8221 catgataaaa aagtaaaaga aaaagaataa accgaacatc caaaagtttg tgttttttaa
     8281 atagtacata atggatttcc ttacgcgaaa tacgggcaga catggcctgc ccggttatta
     8341 ttatttttga caccagacca actggtaatg gtagcgaccg gcgctcagct ggaattccgc
     8401 cgacactgac gggctccagg agtcgtcgcc accaatcccc atatggaaac cgtcgatatt
     8461 cagccatgtg ccttcttccg cgtgcagcag atggcgatgg ctggtttcca tcagttgttg
     8521 ttggctgtag cgactgatat tgaactggaa gtcgccgcgc cactggtgtg ggccataatt
     8581 caattcgcgc gtcccgcagc gcagaccgtt ttcgctcggg aagacgtacg gggtatacat
     8641 gtctgacaat ggcagatccc agcggtcaaa acaggccgca gtaaggcggt cgggatagtt
     8701 ttcttgcggc cctaatccga gccagtttac ccgctctgcg acctgcgcca gctggcaggt
     8761 caggccaatc cgtgccggat gcggtgtatc gctcgctact tcaacatcaa cggtaatcgc
     8821 catttgacca ctaccatcaa tccggtaggt tttccggctg ataaataagg ttttcccctg
     8881 atgctgccac gcgtgagcgg tcgtaatcag caccgcgtcg gcaagtgtat ctgccgtgca
     8941 ctgcaacaac gctgcttcgg cctggtaatg gcccgccgcc ttccagcgtt cgacccaggc
     9001 gttagggtca atgcgggtcg cttcacttac gccaatgtcg ttatccagcg gtgcacgggt
     9061 gaactgagcg cgcagcggcg tcagcagttg tttttcatcg ccaatccaca tctgtgaaag
     9121 aaagcctgac tggcggttaa attgccaacg cttattaccc agctcgatgc aaaaatccat
     9181 ttcgctggtg gtcagatgcg ggatggagtg ggacgcggcg gggagtgtca cgctgaggtt
     9241 ttccgccaga cgccactgat gccaggcgct gatgtgtccg gcttctgacc atgcggtcgc
     9301 gttcggttgc actacgtgta ccgttagcca gagttgcccg gtgctttccg gctgcggtag
     9361 tacaggcagt tcaattacct gtttaccttg tggagcgaca tccagaggca cttcaccgct
     9421 tgccagcggc ttgccatcca gtgccaccgt ccagtgcagg agctcgttat cgctatgacg
     9481 gaacaggtat tcgctggtca cttcgatggt tcgcccggat aaacggaact ggaaaaactg
     9541 ctgctggtgt tttgcttccg tcagcgccgg atgcggcgtg cggtcggcaa agaccagacc
     9601 gttcatgcag aactggcgat cgttcggcgt atcgccaaaa tcaccgccgt aagccgacca
     9661 cgggttgccg ttttcatcat atttaatcag cgactgatcc acccagtccc agacgaagcc
     9721 gccctgtaaa cgggggtact gacgaaatgc ctgccagtat ttagcgaagc cgccaagact
     9781 gttacccatt gcgtgggcat attcgcaaag gatcagcggg cgcagttctc caggcagcga
     9841 aagccatttt ttgatggacc atttcggcac agccgggaag ggctggtctt catccacgcg
     9901 cgcgtacatc gggcaaataa tatcagttgc ggaggtgtcg gctccgccgc cttcatactg
     9961 caccgggcgg gaaggatcga cagatttgat ccagcgatac agtgcgtcgt gattagcgcc
    10021 gtggcctgat tcattcccca gcgaccagat gatcacactc gggtgattac gatcgcgctg
    10081 caccattcgc gttacgcgtt cgctcatcgc gggtagccag cgcggatcat cggtcagacg
    10141 attcattggc accatgccgt gggtttcaat attggcttca tccaccacat acaggccgta
    10201 gcggtcgcac aacgtgtacc acagcggatg attcggataa tgcgaacagc gcacggcgtt
    10261 aaagttgttc tgcttcatca gcaggatatc ctgcaccatc gtctgctcat ccatgacctg
    10321 accatgcaga ggatgatgct cgtgacggtt aacgccgcga atcagcaacg gcttgccgtt
    10381 cagcagcagc agaccatttt caatccgcac ttcgcggaaa ccgacatcgc aggcttctgc
    10441 ttcaatcagc gtgccgtcgg cggtgtgcag tttaaccacc gcacgataga gattcgggat
    10501 ttcggcgctc cacagcgccg ggttttcgac gttcagacgt agcgtgacgc gatcggcata
    10561 accaccacgc tcatcgataa tttcaccgcc gaaaggcgtg gtgccgctgg cgacctgcgt
    10621 ttcaccctgc cacaaagaaa ccgtcacccg cagctcatcg cgcagttcgc cgcacatctg
    10681 aacttctgcc tccagtaccg cgcggctgaa atcatcatta aagcgagtgg caacatggaa
    10741 atcgctgatt tgtgtggtcg gtttgtgcag caacgagacg tcacggaaaa tgccgctcat
    10801 ccgccacata tcctgatctt ccagataact gccgtcactc cagcgcagta ccatcaccgc
    10861 gaggcggttt tctccggcgc gtaaaaatgc gctcaggtca aattcagacg gcaaacggct
    10921 gtcctggccg taaccgaccc agcgcccgtt gcaccacaaa tgaaacgccg aattaacgcc
    10981 atcaaaaata attcgcgtct ggccttcctg tagccagctt tcatcaacat taaatgtgag
    11041 cgagtaacaa cctgtcggat tctccacggg aacaaacggc ggattgaccg taatgggata
    11101 ggtcacgttg gtgtagatgg gcgcgtcgta accgtgcatc tgccagtttg aggggacgac
    11161 gatagtatcg gcgtcaggaa gatcgcactc cagccagctt tccggcaccg cttctggtgc
    11221 cggaaaccag gcaaagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11281 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgccg caaggcgatt
    11341 aagttgggta acgccagggt tttcccagtc acgacgttgt aatacgacgg ccagtgaatc
    11401 cgtaatcata gtcatagctg tatcctgtgt gaaattgtta tccgctcaca attccacaca
    11461 acatacgagc cggacgcata aagtgtaaag cctggggtgc ctaatgagtg agctgactca
    11521 cattaattgc gttgcgctca ctgcccgctt tcgagtcggg aaatttgtcg cgccagctgc
    11581 attaaagaat ctgccaacgc ctggggagaa gcggtttgcg tattgggcgg aagggtggtt
    11641 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
    11701 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
    11761 gttaacggcg ggatataaca cgagctgtct tcggtatcgt cgtatcccac taccgagata
    11821 tccgcaccaa cgtgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
    11881 tcgttggcaa ccagcatcgc agtaggaacg atgtcctcat tcagcatttg catggtttgt
    11941 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
    12001 cgagtgagat atttatgcca gcccgccagg cgcagacgtg ccgagacaga acttaatgga
    12061 cctgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
    12121 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
    12181 aataaagccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
    12241 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
    12301 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
    12361 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
    12421 gcaacgccaa tcagcaacga ctgcttgccc gccagttgtt gtgccacgcg gttgggaatg
    12481 taattcagct ccgccatcgc cgcttctact ttttcccgcg ttttcgcaga aacgtggctg
    12541 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
    12601 tatagcgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
    12661 gccataccgc gaaaggtttt gcgccattcg atggtgtcaa cgtaaatgca tgccgcttcg
    12721 ccgtccggcc accagaatag cctgcgattc aaccccttct tcgatctgtt ttgccacccg
    12781 ttgtagcgcc ggaagatgct tttccgctgc ctgttcaatg gtcattgcgc tcgccatata
    12841 caccagattc agacagccaa tcacccgttg ttcactgcgc agcggtacgg caatagaggc
    12901 gatcttttct tcttgatccc agccgcggta gttctgtccg tacccctctt tgcgcgcgcg
    12961 cgccagaatg gcttccagct ttaacggttc ccgtgccagt tgatagtcat caccggggcg
    13021 ggaggctaac atctcgatta attccttgcg ttcttgttcc gggcaaaagg ccagccaggt
    13081 caggcccgag gcggttttca ggagcggtaa acgtcgcccg accattgccc ggtgaaagga
    13141 taagcggctg aaacggtgag tggtttcgcg taccaccatt gcatcaacat ccagcgtgga
    13201 cacatctgtc ggccatacca cttcgcgcaa cagatcgccc agcagtgggg ccgccagtgc
    13261 agaaatccac tgttcgtcac gaaatccttc gcttaattgc cgcactttga tggtcagacg
    13321 aaaactgtca tcggagggac tacgccggac atatccctct tcctgcagcg tctccagcag
    13381 tcgccgcaca gtggtgcgat gcaggccgct gagttccgcc agcagcccga cgctggcacc
    13441 gccatcaagt ttatttaaca tatttaataa cattagaccg cgggttaagc cgcgcacggt
    13501 tttgtattcc gtctgctcat tgttctgcat attaattgac atttctatag ttaaaacaac
    13561 gttgtgcacc tggtgcacat tcgggcatgt tttgattgta gccgaaaaca cccttcctat
    13621 actgagcgca caataaaaaa tcatttacat gtttttaaca aaataagttg cgctgtactg
    13681 tgcgcgcaac ggcattttgt ccgagtcgtg aggtactgaa atggcaatac aacaccctga
    13741 catccagcct gctgttaacc atagcgttca ggtggcgatc gctggtgccg gtccggtcgg
    13801 gctgatgatg gcgaactatc tcggccagat gggcattgac gtgctggtgg tggagaaact
    13861 cgataagttg atcgactacc cgcgggcgat tggtattgat gacgaggcgc tgcgcaccat
    13921 gcagtcggtt ggcctggtcg ataatgttct gccgcacact acgccgtggc acgcgatgcg
    13981 ttttctcacc ccgaaaggcc gctgttttgc tgatattcag ccaatgaccg atgaatttgg
    14041 ctggccgcgc cgtaacgcct ttattcagcc tcaggtcgat gcggtgatgc tggaaggatt
    14101 gtcgcgtttt ccgaatgtgc gctgcctgtt ttcccgcgag ctggaggcct tcagccagca
    14161 agatgacgaa gtgaccttgc acctgaaaac ggaagaaggg cagcgggaaa cggtcaaagc
    14221 ccagtggctg gtggcctgtg atggtggggc aagttttgtc cgtcgcaccc tgaatgtgcc
    14281 gtttgaaggt aaaactgcgc caaatcagtg gattgtggta gatatcgcca acgatccgtt
    14341 aagtacgccc catatctatt tgtgttgtga tccggtgcgc ccgtatgttt ctgccgcgct
    14401 gcctcatgcg gtacgtcgct ttgaatttat ggtgatgccg ggagaaaccg aagaacagct
    14461 gcgtgagccg caaaatatgc gcaagctgtt aagcaaagtg ctgcctaatc cggacaatgt
    14521 tgaattgatt cgccagcgtg tctataccca taacgcgcga ctggcgcaac gtttccgtat
    14581 tgatcgcgta ctgctggcgg gcgatgccgc gcacatcatg ccggtgtggc aggggcaggg
    14641 ctataacagt ggtatgcgcg acgcctttaa cctcgcatgg aaactggcgt tggttatcca
    14701 ggggaaagcc cgtgatgcgc tgctcgatac ctatcaacaa gaacgtcgcg atcacgccaa
    14761 agcgatgatt gacctgtccg tgacggcggg caacgtgctg gctccgccga aacgctggca
    14821 gggtacgtta cgtgacggcg tttcctggct gttgaattat ctgccgccag taaaacgcta
    14881 cttcctcgaa atgcgcttca agccgatgcc acaatattac ggcggtgcgc tggtgcgtga
    14941 gggcgaagcg aagcactctc cggtcggcaa gatgtttatt cagccgaaag tcacgctgga
    15001 aaacggcgac gtgacgctgc tcgataacgc gatcggcgcg aacttcgcgg taattggctg
    15061 gggatgcaat ccactgtggg ggatgagcga cgagcaaatc cagcagtggc gcgcgttggg
    15121 cacacgcttc attcaggtgg tgccggaagt gcaaattcat accgcacagg ataaccacga
    15181 cggcgtacta cgcgtgggcg atacgcaagg tcgcctgcgt agctggttcg cgcaacacaa
    15241 tgcttcgctg gtggtgatgc gcccggatcg ctttgttgcc gccaccgcca ttccgcaaac
    15301 cctgggcaag accctgaata aactggcgtc ggtgatgacg ctgacccgcc ctgatgccga
    15361 cgtttctgtc gaaaaggtag cctgatatgc acgcttatct tcactgtctt tcccactcgc
    15421 cgctggtggg gtatgtcgac ccggcgcaag aggtgctcga tgaggtcaat ggcgtgattg
    15481 ccagcgcccg cgagcgtatt gcggctttct cccctgaact ggtggtgctg tttgcgccag
    15541 atcactacaa cggctttttc tatgacgtga tgccaccgtt ctgtttaggc gttggagcga
    15601 cggcaattgg tgatttcggt agtgcggcag gagagctgcc cgtgcctgtt gagctggcgg
    15661 aggcctgtgc gcatgccgtc atgaagagcg ggatcgatct tgccgtttct tactgtatgc
    15721 aggtggacca cgggttcgcc cagccgctgg agttcctgct cggcgggctg gataaggtgc
    15781 cggttttgcc tgtgttcatc aacggtgtcg ccacgccgtt gcccggtttc cagcgtaccc
    15841 gcatgttggg tgaagccatt ggacgtttca ccagtactct caataaacgc gtgctgttcc
    15901 tgggttccgg tgggctttcc catcagccgc cggtgccaga actggcgaaa gccgatgccc
    15961 atatgcgcga ccgtctgttg gggagcggga aagatttacc cgccagtgag cgcgaattgc
    16021 gtcagcaacg ggtgattagc gccgctgaga agtttgttga ggatcagaga acgctgcatc
    16081 ccctcaatcc gatttgggat aaccagttca tgactttgct ggagcaggga cgcatacagg
    16141 aactggatgc cgtcagtaac gaagagcttt ccgccattgc cggaaagtcg acacatgaaa
    16201 tcaaaacctg ggtcgccgct tttgccgcta tttctgcgtt tggcaactgg cgtagcgaag
    16261 ggcgttatta ccgcccaatc ccggagtgga ttgccggatt tggctcgtta agcgccagaa
    16321 cagagaactg aatatgcagg agaagatgat gagttatcag ccacaaaccg aagccgccac
    16381 cagtcgtttt ctgaatgtag aagaagcggg taaaacgctg cgcatccatt ttaatgactg
    16441 cggacaaggc gacgaaactg ttgtcctgct gcatggttcc ggcccgggtg ctactggctg
    16501 ggcgaacttc agccgcaata tcgatccgct ggtagaggcg ggctatcggg tgatcctgct
    16561 ggattgtccg ggttggggta agagcgattc gatcgttaat agtggttcgc gatcggatct
    16621 taatgcgcga atcctgaaaa gcgtggtgga tcaactggat atcgccaaaa tccacctgct
    16681 gggcaactcg atgggcggcc acagttctgt ggcgttcact cttaactggc cggagcgcgt
    16741 cggcaaactg gtgctgatgg gcggcggtac gggcggcatg agtttgttta cgccgatgcc
    16801 aaccgaaggt attaagcgac tgaatcagct ttatcgtcag ccgactatcg aaaacctgaa
    16861 gctgatgatg gatatcttcg tttttgatac cagcgatttg accgacgccc tgtttgaagc
    16921 gcgcctgaat aatatgctgt cgcgccgcga tcacctggaa aacttcgtta agagcctgga
    16981 agccaatccg aaacagttcc cggattttgg cccacgtctg gcggaaatca aagcgcaaac
    17041 cctgattgtc tgggggcgca acgaccgctt tgtgccgatg gatgcgggtc tgcgtctgct
    17101 gtccggcatt gccggttctg aactgcatat cttccgcgac tgtggtcact gggcgcagtg
    17161 ggaacatgcc gacgctttca atcaactggt gctgaatttc ctcgcacgcc cttaaggaac
    17221 ggtcatgacg aagcatactc ttgagcaact ggcggcggat ttacgccgcg ccgcagagca
    17281 gggcgaagcg attgcgccgc tgcgcgatct gattggtatt gaaaacgctg aagcggccta
    17341 cgccattcag cgcatcaatg tgcaacatga cgttgcgcag gggcgtcgcg tggtggggcg
    17401 taaagtgggc ctgacgcatc cgaaagtgca gcaacaactg ggcgttgatc aaccggattt
    17461 cgggacatta tttgccgaca tgtgttatgg cgataacgaa accattcctt tttcccgtgt
    17521 tctgcaaccc cgcattgaag cggagattgc actggtgttg aaccgcgatt tgcccgcaac
    17581 cgatatcacc ttcgacgaac tgtataacgt cattgaatgg gtacttccgg cgctggaggt
    17641 ggtgggtagc cgcattcgcg actggtcgat tcagtttgtc gataccgtgg cagataacgc
    17701 ctcctgtggg gtgtatgtca tcggcggtcc ggcgcaacgt ccggcggggt tagacctgaa
    17761 aaactgcgcc atgaagatga cgcgtaataa cgaagaggtt tccagcgggc gcggcagcga
    17821 atgcctggga catccgctta atgccgccgt ctggctggca cgcaaaatgg ccagcctggg
    17881 tgaaccgctg cgcgccggag atatcattct taccggggca ttaggtccga tggtggcggt
    17941 gaatgcgggc gatcgttttg aagcccatat cgaaggcata ggttcagttg ctgcgacatt
    18001 ttcaagcgca gccccaaaag gaagtctgtc atgagtaagc gtaaagtcgc cattatcggt
    18061 tctggcaaca ttggcaccga tctgatgatt aaaattttgc gtcacggtca gcatctggag
    18121 atggcggtga tggtcggcat tgatcctcag tccgacggtc tggcgcgtgc cagacgtatg
    18181 ggcgtcgcca ccacccatga aggggtgatc gggctgatga acatgcctga atttgctgat
    18241 atcgacattg tatttgatgc gaccagcgcc ggtgctcatg tgaaaaacga tgccgctttg
    18301 cgcgaagcga aaccggatat tcgcttaatt gacctgacgc ctgctgccat cggcccttac
    18361 tgcgtgccgg tggttaacct cgaggagaac gtcgatcaac tgaacgtcaa catggtcacc
    18421 tgcggcggcc aggccaccat tccaatggtg gcggcagttt cacgcgtggc gcgtgttcat
    18481 tacgccgaaa ttatcgcttc tattgccagt aaatctgccg gacctggcac gcgtgccaat
    18541 atcgatgaat ttacggaaac cacttcgcgc gccattgaag tggtgggtgg cgcggcaaaa
    18601 gggaaggcaa ttatcgtgct taacccagca gagccaccgt tgatgatgcg tgacacggtg
    18661 tatgtattga gcgacgaagc ttcacaagat gatatcgaag cctcaatcaa tgaaatggct
    18721 gaggcggtgc aggcttacgt accgggttat cgcctgaaac agcgcgtgca gtttgaagtt
    18781 atcccgcagg ataaaccggt taatttgccg ggcgtggggc agttctccgg gctgaaaaca
    18841 gcggtctggc tggaagtcga aggcgcagcg cattatctgc ctgcctatgc gggcaacctc
    18901 gacattatga cttccagtgc gctggcgaca gcggaaaaaa tggcccagtc actggcgcgc
    18961 aaggcaggag aagcggcatg aacggtaaaa aactttatat ttcggacgtc acgttgcgtg
    19021 acggtatgca cgccattcgt catcagtatt cgctggaaaa tgtccgccag attgccaaag
    19081 cactggacga tgcccgcgtg gattcgattg aagtggccca cggcgacggt ttgcaaggtt
    19141 ccagctttaa ctatggtttc ggcgcacata gcgacctaga atggattgaa gcagcggcgg
    19201 atgtggtgaa gcacgccaaa atcgcgacgt tgttgctgcc aggaatcggc actattcacg
    19261 atctgaaaaa tgcctggcag gctggcgcgc gggtggttcg tgtggcaacg cactgtaccg
    19321 aagctgatgt ttccgcccag catattcagt atgcccgcga gctcggaatg gacaccgttg
    19381 gttttctgat gatgagccat atgaccacgc cggagaatct cgcaaagcag gcaaagctga
    19441 tggaaggcta cggtgcgacc tgtatttatg tggtggattc tggcggtgcg atgaacatga
    19501 gcgatatccg tgaccgtttc cgcgccctga aagcagagct gaaaccagaa acgcaaaccg
    19561 gcatgcacgc tcaccataac ctgagtcttg gcgtggcgaa ctctatcgcg gcggtggaag
    19621 agggctgtga ccgaatcgac gccagcctcg cgggaatggg agcgggcgca ggtaacgcgc
    19681 cgctggaagt atttattgcc gccgcggata aactgggttg gcagcatggg accgatctct
    19741 atgcgttaat ggatgccgct gacgacctgg tgcgtccgtt gcaggatcga ccggtacgag
    19801 tcgatcgcga aacgctggcg ctgggatacg ctggtgttta ctccagcttc ctgcgtcact
    19861 gtgaaacggc ggcggcgcgt tatggcttaa gcgcggtgga tattctcgtt gagttgggca
    19921 aacgccggat ggttggcggt caggaggata tgatcgtcga tgtggcgctg gatctgcgca
    19981 acaacaaata atgatgactg ccgagaacgt gcattttgta tgcccatccc agcacgactg
    20041 ccggatgcgg cgtaaacgcc ttatccggcc tatatttcgc ccgtaggcag tcattaaatg
    20101 gttctgatta cgcggctgac aatgtatcag ccgcgattca cctgcactcg caacgaggtt
    20161 catcttatgt cgactcgtac cccttcatca tcttcaaccc gcctgatgct gaccatcggg
    20221 ctttgttttt tggtcgctct gatggaaggg ctggatcttc aggcggctgg cattgcggcg
    20281 ggtggcatcg cccaggcttt cgcactcgat aaaatgcaaa tgggctggat attcagcgcc
    20341 ggaatactcg gtttgctacc cggcgcgctg gttggcggaa tgctggcgga ccgttatggt
    20401 cgtaagcgta ttttaattag ctcagttgcg ctgtttggtt tgttctcact ggcaacggcg
    20461 attgcctggg atttcccctc actggtcttt gcgcgactga ttaccggtgt cgggctgggg
    20521 gcggcgttgc cgaatctgat cgccctgacg tctgaagccg cggggccacg ttttcgtggg
    20581 acggcagtga gcctgatgta ttgcggtgtt cccattggcg cggcgctggc ggcgacactg
    20641 ggtttcgcgg gggcaaactt agcatggcaa acggtgtttt gggtaggtgg tgtggtgccg
    20701 ttgattctgg tgccgctatt aatgcgctgg ctgccggagt cggcggtgtt cgctggcgaa
    20761 aaacaggccg cgcctccact gcgtgcgtta tttgcgccag aaacggcaac cgcgacgctg
    20821 ctgctgtggt tgtgttattt cttcactctg ctggtggtct acatgttgat caactggcta
    20881 ccgctgcttt tggtggagca aggattccag ccatcgcagg cggcaggggt gatgtttgca
    20941 ctgcaaatgg gtgcggcaag cgggacgtta atgttgggcg cattgatgga taagctgcgt
    21001 ccagtaacca tgtcgctact gatttatagc ggcatgttag cttcgctgct ggcgctggga
    21061 acggtgtcgt catttaacgg tatgttgctg gcgggattcg tcgcggggtt gtttgcgaca
    21121 ggtgggcaaa gcgttttgta tgccctggca ccgttgtttt acagttcgca gatccgcgca
    21181 acaggtgtgg gaacagccgt ggcggtaggg cgtctggggg ctatgagcgg tccgttactg
    21241 gccgggaaaa tgctggcatt aggcactggc acggttggcg taatggccgc ttctgcgccg
    21301 ggtattcttg ttgctggcct ggcagtgttt attttgatga gccggagatc acgaatgcag
    21361 ccgtgcgccg atgcctgacg cgccttatta agcagggacg aaagggactt gccccaatac
    21421 acctacggcg tttacaggta tactcgctaa aaattattca gcgggtttgg aaacaaagat
    21481 ggcaaaactt accttacaag agcagttgct caaagcagga ttagtcacca gcaaaaaagc
    21541 ggcgaaggtc gagagaacgg cgaaaaaatc gcgcgttcag gcgcgtgaag ctcgggcggc
    21601 ggtagaagaa aataaaaagg cacagcttga acgtgataaa cagcttagcg agcagcaaaa
    21661 acaagcggcg ttggcgaaag aatataaagc tcaggtgaag cagctcattg aaatgaaccg
    21721 aatcaccatt gttaatggcg atattggttt taacttcact gacggtaatc tgattaagaa
    21781 aatctttgtc gataagctca cgcaagcgca gttaattaat ggtcgtctgg cgattgcccg
    21841 cttgttgctc gataacaata gcgaaggtga atacgccatt attcccgcca gcgttgccga
    21901 taaaattgct cagcgtgatg ccagcagtat tgtgttgcac agcgcgctca gtgccgaaga
    21961 gcaggatgaa gacgatccgt atgccgactt caaagtgcct gatgatttga tgtggtaaac
    22021 atagataagt cgtgcgcaga tgcctgatgc gacgctatgc gcgtcttatc atgcctacgg
    22081 cctgcgggcg aaatgtaggc ggataaggcg ttcacgccgc atccggcagt cgtgcactat
    22141 tattaacgca tattcagttt attggcgtga taggcaatat gctcgccaat aaaactggag
    22201 acaaaataat agctgtgatc ataaccctcg tgataacgga ttaacgtctt gatattcatc
    22261 tcctggcaga tcttttcaag atttggagtc cgcagctgtt ctgcgtaaaa atcatcactc
    22321 aacccctgat caaccatgat ttccgcaacg cgttgacctt gtgaaataag gctcaccggg
    22381 tcgtaatcca accaggcttc tttattttca ccaagatatg cggtaaaggc ttgctgtccc
    22441 cacggtactt gcgatgggga gacaatgggc gaaaacgctg agacgctgac atattcatct
    22501 ggattacgta acgccagcac cagtgcgccc agcccgccca tagaatgacc tgagatagac
    22561 tttctggccg ttgccggaaa atgttgcatc actaaatccg gcagctcgtt gcggatatag
    22621 tcatacattt tgtaatgttc attccacggc gcttgcgttg cgttcaggta aaacccggca
    22681 ccttgcccga gatcgtaacg gtcagcatct gcgacatgac tgcctcgcgg actggtgtcc
    22741 ggcgcaacaa caataatgtt gtgctcagcc gcgtaacgtt gcatccccga tttagtaatg
    22801 aaattctgct cgttgcaagt cagccctgaa agccagtaca acaccggcaa tttttcactt
    22861 gcggcttttg gtgggagata gacgccgaca ttcatttcac atttcagtga tggggaataa
    22921 tgccgataca cattttgcca gccgccaaag ctggcatgtt tttcaatgag ttccatcagt
    22981 cattatctca cggttaatcc cccggacggc tgcccgggga gagtattaag aaccccgtct
    23041 gaactggacg ggtaaacctg cgggaaatca gtaacgaatt acggttcgaa tggatttgcc
    23101 ttcatgcatc aggtcaaagg cgtcattaat ttcatccagg ctcatggtat gcgtgacaaa
    23161 cggttccaga tcgatatcgc ctttcatcgc atcttcaacc atacccggta actgggaacg
    23221 acctttcacg ccgccaaacg cggaaccttt ccatacgcga ccggtgacca actggaatgg
    23281 acgggtggag atttcctgac cagaaccagc taccccgatg atcaccgact gaccccagcc
    23341 gcggtgcgca ctttccagcg ccgcacgcat cacgttgacg ttaccgatgc attcaaaggt
    23401 atggtcgata ccccatttgt tgatatccag caggacatct ttaatcggtt tgtcgtagtc
    23461 attcgggtta atgcagtcgg tggcaccgaa gcgacgcgcc agatcgaatt tcttcgggtt
    23521 cgtatcaata gcgataatcc gtcccgcttt cgcctgacgc gcgccctgaa ccactgccag
    23581 accaatcgcg ccaagaccaa atacggcaac agaatcacct ggctggactt tagccgtgtt
    23641 gtgtaccgcg ccaataccgg tggtcacgcc acagcccagc aggcagacgt gttcatggtt
    23701 tgcttctgga ttaattttgg ccagagacac ttccgcgacc acggtgtatt cactgaatgt
    23761 agagcatccc atgtagtgat aaagcggctg cccgttgtaa gaaaaacggg tggtgccgtc
    23821 tggcatcagg cctttaccct gggtttcgcg aaccgcaaca cagaggttgg ttttgccgga
    23881 acgacagaac tcacactcgc cgcactctgc ggtgtaaagc gggatcacat ggtcgccagg
    23941 tttgacgctg gttacgcctt caccgacttc aaccacaacg ccggcccctt cgtgaccgag
    24001 aactaccggg aatacacctt ccgggtcatc gccggagagg gtaaatgcgt cggtatggca
    24061 aacgccggta tgggtgactt taatcagcac ttcacctttt ttcggtggtg caacgtcaat
    24121 ttcaacgatt tccagcggtt taccgggagc aaatgcaacg gcagcacgtg atttcatctc
    24181 tcgctcttcc tcaatatggt aatagattca gctatttaag ataggcacga accagttcaa
    24241 tagtgtcgtc aacggattgg ctgacttcgc ggctgtagca gtcatttcgg tcaaacgttt
    24301 cccggatatg gctttcaagc acttctgcca tcagcccgtt agccgcgccc cgaacggcag
    24361 cgatctgttg gagtatggca cggcattcgg catcaccctc cagcgaccgt tccagagcat
    24421 caatctgccc ccgaatacga cgaactcgag taaggacctt tttcttctct tccggagtac
    24481 tgggcatttc gcacctcatc atctgcacgc aatatactat aggggggtat gctatatgtc
    24541 aatgcatacc cccctatagt atataacact ggagaataaa atttatccgg tgaatgtggt
    24601 cggaaaacaa agaggaaagg gggtggggct aatcggcagg gaaggccgcc ccggatagcg
    24661 ggcggcagaa gcaatcagaa tttccaggtc agatgggctg caagttgcag accgttataa
    24721 tcatcggttg gggtgtcgta ccacacttta cctgccgtca gcccgagatt aagttgctca
    24781 gtcagcggtt gaatacgttg caaactgacg ctcttcatgc tgccgtagcg tgtttctggc
    24841 gtccagtcat aggtgtaagc acctgttccg cggcttagcc atagttgcgt ataaccagtg
    24901 ccgcgcgggt cattcagacg cacggaaatc atattgctgt aactaccacc tgcatcgctg
    24961 gagtcataat gggtatagcg gtagctggag atcaccgggc cagtatagag tgatacgccg
    25021 ccttgccagg catcgacttc gacatcatcg taatatttag tgtagcgata accggtcgtg
    25081 aaaagcgttt ttggcaacag tgccaggtta atatcctgac gaaaatcctg gcgggcaaag
    25141 accggcgtat tatccgcaaa ggcaataccc gttcgggttg tcagccagtt attccattta
    25201 taccagaccg caccctgacc gcgagtggca ttgaaatgtt cagtttcata atcacgacgc
    25261 ccctgggaaa gattaaaaag cagcgttgcg ttttcaactt tcgccaccag ttcagcatac
    25321 gctaaattac ggttgccatg atcgccagaa taatcggtaa aatcgtagcc cgcggtaatg
    25381 gaagttaacc cggcataagc gggcaatgtg ctaaaaattg ctgccgctaa taacgtccgt
    25441 tttatcattt taatctcctg tacggataag ttcttgtcgg agtgaataag ttccgggagg
    25501 taaattacgt tctacaatca cgcgcgggcc aagttgcgtg ttcggggaaa taattcgccc
    25561 aggcaaaata ataacttgta cgccaaggcg tgaacgctgc ccgatataac aacctaattt
    25621 atcgcatccg gtagcgataa ttccctctgg agttcgaaca gacacgggct gttgatccag
    25681 acgatgatta ctggtacgta cttgtgcgcc caaatatgcc tggtttgcaa ctaccgagtc
    25741 ggcgataaag cattgcggac caatcgttgc ttccgcttca ataaccgcat ttttaatttc
    25801 ggtggcgaaa ccaattttta cgccattgct gattattgtg ccaggacgaa taaacgcata
    25861 attacctatc aggcagtttg cgccaatcac taccggaccc tgaataacgg caccgtgaca
    25921 aatacgggta ttcgcgccga taacaacttc acctgcagtt tcatcaataa ttacctggtc
    25981 tgcaatctga atatttttct ctttaacatt aagcttcaga aagtttttca gttcctgtga
    26041 cagaaggttg ggaataagag gttgacgctt ccaccagtgc ggaatagcgg gtgggtttgt
    26101 cgcgctgagg ttcgcgtcca gtaaaaaagg caataagtcc ataaataatc cagatggcat
    26161 aagctaataa tacatacaca acggaaagcg gtgctaaagg caccaacaac cagcaacgat
    26221 gaaaccaggc gctaaaagca ccaataacac aaactacgcc gacccagata agcggaaaca
    26281 ttgccaacac cacaccatac ggcccggtgg tgatgaattc attaaaccag gtagtgagat
    26341 aaatcccaac gccataaagc acaaccaaca gcataggaaa tatactgaag ataccgaagc
    26401 ggctaaataa aagcctttta tgcaggcgca tacagaccgc gtagcccaca atccaacgcc
    26461 gccagcgacg ccattcctca cgcgggctgt tgcattcctg tgggtatacg atgcagcgat
    26521 tcgcctgccg aatacgatag ccgtttgcca ccaatgtcca ggtgagatca aggtcttcga
    26581 ctttagtacg atcagagaaa ccgaacttac gcaatacatc agtacggaac atcccgcagg
    26641 caccgctgat aataaacggt gcgccgccca ggagctgctg tagcgtgcgc ttcataacaa
    26701 tcatcggcaa ctttacggtc gcgcggatgt gcggtaacag acccgcgcct ttcaacgcag
    26761 tagagggaat gccgcctacg gcatcagcac cgcgctctat ttctgcaagc atatagccca
    26821 ttccgtcttg atcgggcgga acataggtgt cggcatcact taaaaaaacc tggtcgcagg
    26881 tggcgtaatt gaggccattc atcagcgcac cacctttacc ggtatttttt tgcgtgacgg
    26941 caacaaagcg gtcgccccat ttgcgtttga cttccgccat gaccgcttcg gtattgtccg
    27001 tggagccgtc gtttacgcaa attacccggc aaaaataagg attacgcagc agattatcca
    27061 gtgactgtgc cagacacggg ccttcgttat aagcaggtat aatggcgtca atacagcctt
    27121 tcttttgact gggtttacgt tttagcgtac ttaaaaacca tagcaacatt gctatggaca
    27181 tacagataaa tatccaggtt ttcatgcaat ttgctcaa
//
LOCUS       DAYPHN010000056        27011 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000056 DAYPHN010000000
VERSION     DAYPHN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.056"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            82..1302
                     /locus_tag="ACG0BW_002223"
     CDS             82..1302
                     /locus_tag="ACG0BW_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFK7259176.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWIALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YASHQRLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1275..1904
                     /locus_tag="ACG0BW_002224"
     CDS             1275..1904
                     /locus_tag="ACG0BW_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFK7259177.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1905..3065)
                     /gene="ybdL"
                     /locus_tag="ACG0BW_002225"
     CDS             complement(1905..3065)
                     /gene="ybdL"
                     /locus_tag="ACG0BW_002225"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFK7259178.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVDQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     QAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3173..4261
                     /gene="hcxA"
                     /locus_tag="ACG0BW_002226"
     CDS             3173..4261
                     /gene="hcxA"
                     /locus_tag="ACG0BW_002226"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFK7259179.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSLNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNHQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4271..4468)
                     /locus_tag="ACG0BW_002227"
     CDS             complement(4271..4468)
                     /locus_tag="ACG0BW_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFK7259180.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4622..6727)
                     /gene="cstA"
                     /locus_tag="ACG0BW_002228"
     CDS             complement(4622..6727)
                     /gene="cstA"
                     /locus_tag="ACG0BW_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFK7259181.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6908..7321)
                     /gene="entH"
                     /locus_tag="ACG0BW_002229"
     CDS             complement(6908..7321)
                     /gene="entH"
                     /locus_tag="ACG0BW_002229"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFK7259182.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDN
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7324..8070)
                     /gene="entA"
                     /locus_tag="ACG0BW_002230"
     CDS             complement(7324..8070)
                     /gene="entA"
                     /locus_tag="ACG0BW_002230"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFK7259183.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8070..8927)
                     /gene="entB"
                     /locus_tag="ACG0BW_002231"
     CDS             complement(8070..8927)
                     /gene="entB"
                     /locus_tag="ACG0BW_002231"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFK7259184.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLNDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8941..10551)
                     /gene="entE"
                     /locus_tag="ACG0BW_002232"
     CDS             complement(8941..10551)
                     /gene="entE"
                     /locus_tag="ACG0BW_002232"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFK7259185.1"
                     /translation="MSIPFTRWPEEFSRRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10561..11736)
                     /gene="entC"
                     /locus_tag="ACG0BW_002233"
     CDS             complement(10561..11736)
                     /gene="entC"
                     /locus_tag="ACG0BW_002233"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFK7259186.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAELFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QSSARRFTRSQSLNVVERQAIPDQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHIPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSHENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11925..12881
                     /gene="fepB"
                     /locus_tag="ACG0BW_002234"
     CDS             11925..12881
                     /gene="fepB"
                     /locus_tag="ACG0BW_002234"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFK7259187.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIVAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12885..14135)
                     /gene="entS"
                     /locus_tag="ACG0BW_002235"
     CDS             complement(12885..14135)
                     /gene="entS"
                     /locus_tag="ACG0BW_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFK7259188.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14246..15250
                     /gene="fepD"
                     /locus_tag="ACG0BW_002236"
     CDS             14246..15250
                     /gene="fepD"
                     /locus_tag="ACG0BW_002236"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFK7259189.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15247..16239
                     /gene="fepG"
                     /locus_tag="ACG0BW_002237"
     CDS             15247..16239
                     /gene="fepG"
                     /locus_tag="ACG0BW_002237"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFK7259190.1"
                     /translation="MIYVSRRLIITCLLLITACVVAGIWGLRSGTVTLETSQIFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16236..17051
                     /gene="fepC"
                     /locus_tag="ACG0BW_002238"
     CDS             16236..17051
                     /gene="fepC"
                     /locus_tag="ACG0BW_002238"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFK7259191.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17063..18181)
                     /gene="wzz(fepE)"
                     /locus_tag="ACG0BW_002239"
     CDS             complement(17063..18181)
                     /gene="wzz(fepE)"
                     /locus_tag="ACG0BW_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFK7259192.1"
                     /translation="MSSLNIKQGSDTHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACTGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYNSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSAMIGGMVACGGVLLRHAMASRKQDAVM"
     gene            complement(18397..22278)
                     /gene="entF"
                     /locus_tag="ACG0BW_002240"
     CDS             complement(18397..22278)
                     /gene="entF"
                     /locus_tag="ACG0BW_002240"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFK7259193.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTSFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAMQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIHGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALVDARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPETARQSCATLKQVFCSGEALPADLCREWQQLTGV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMLHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFTPGERMYRTGDVARWLENGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQATATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRTRGEQVAFLGLLDTWPPETQNWQKKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPVAFEKIGPIIRATLNK"
     gene            complement(22275..22493)
                     /gene="ybdZ"
                     /locus_tag="ACG0BW_002241"
     CDS             complement(22275..22493)
                     /gene="ybdZ"
                     /locus_tag="ACG0BW_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFK7259194.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPSNFTQLQEAQ"
     gene            complement(22496..23698)
                     /gene="fes"
                     /locus_tag="ACG0BW_002242"
     CDS             complement(22496..23698)
                     /gene="fes"
                     /locus_tag="ACG0BW_002242"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFK7259195.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYVTGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPVKEIIWKSEQLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23941..26181
                     /gene="fepA"
                     /locus_tag="ACG0BW_002243"
     CDS             23941..26181
                     /gene="fepA"
                     /locus_tag="ACG0BW_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFK7259196.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            26356..26976
                     /gene="entD"
                     /locus_tag="ACG0BW_002244"
     CDS             26356..26976
                     /gene="entD"
                     /locus_tag="ACG0BW_002244"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFK7259197.1"
                     /translation="MKTTHTALPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKYVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKI
                     QAAQGFLDYQIISWNKQRIIIRLEDEQFAVHWQIKEKIVITLCQHD"
BASE COUNT         6420 a         7610 c         7020 g         5961 t
ORIGIN      
        1 tgctctatct tgcttcgttt accctataat taacggattc atctcacgag tcaccccgta
       61 acgttcagag agcgttttca gatgtctatt tataaaatcc cgcttccact gaatattctg
      121 gaagctgcaa aagaacgtat tacctggaca cttaacaccc taccccgcgt ctgcgtttct
      181 ttttcgggtg gtaaagattc cggattaatg ctgcatctga ccgccgaact tgcccgacaa
      241 atgggcaaaa aaatttgtgt tttatttatc gactgggaag ctcagttctc ttgcaccatt
      301 aactatgttc agtctctgcg cgagttgtac accgatgtca tcgaagagtt ttactggatt
      361 gcgctcccgc ttacaacgca aaattcccta tcacaatacc aacccgaatg gcagtgctgg
      421 gaacctgatg tcgaatgggt acgtcagccc ccgcaagatg cgataaccga tcctgacttt
      481 ttctcctttt accagcctgg catgaccttc gaacaatttg tgcgtgagtt tgccgaatgg
      541 ttttcgcaaa aacgtccggc ggcgatgatg atcggcatcc gtgcggatga gtcctacaac
      601 cgttttgtcg ccatcgccag tttaaataaa caacgttttg ccgatgataa gccctggact
      661 actgccgcac caggcggtca tagctggtac atttacccca tctacgactg gaaagtcgct
      721 gatatctgga cctggtatgc tagccatcaa cgcctctgta acccactgta taatataatg
      781 tatcaggcag gcgttcctct acgtcatatg cgaatttgcg aaccttttgg tccggaacaa
      841 cggcaaggat tatggctcta tcacgttatc gaaccggatc gctgggctgc tatgtgcgca
      901 cgagtcagcg gggtaaaaag tggcggcatt tacgccggac atgacaacca tttttatggt
      961 catcggaaaa tcctcaagcc cgaacattta gactggcagg agtatgcatt attgctactc
     1021 aacagcatgc cggagaaaac agctgagcat tatcgcaata aaatcgccat ttatttgcac
     1081 tggtatcaga aaaaaggcat cgaggttcca caaacccagc aaggggacat tggcgcgaaa
     1141 gatatcccct cctggcggcg aatatgcaaa gttttactca ataacgatta ctggtgtcgg
     1201 gcattatcat ttagcccgac caaagcgaag aactatcagc gttataacga acgaataaaa
     1261 ggaaaacgtc aggaatgggg gatactatgc aacaacgatt aacgcaggat ttaacccaat
     1321 ttctcgccag cttgccggaa gacgatcgca tcaaagcgat taatgaaatc cgcatggcga
     1381 tccatcaggt tagccccttt cgagaagagc cagtggattg cgttctgtgg gttaaaaaca
     1441 gccaacttat gcctaatgat tacaacccaa acaacgtggc accacccgaa aaaaaactgc
     1501 tgaaaaaatc cattgaaata gatggtttta cccaacccat tgtggtcacg catacggata
     1561 aaaatgccct ggaaatcgtg gatggttttc accgccatga aattggtaaa ggctcatcat
     1621 cgttaaaatt acgcttaaaa ggttatttgc cggtgacctg tctggaaggt acgcgtaatc
     1681 aacgcatcgc cgcgactatt cgtcataacc gcgctcgtgg acgtcaccaa atcactgcca
     1741 tgtcagaaat cgtccgtgaa ctcagccagt tgggatggga cgataataaa attggcaaag
     1801 agctgggaat ggacagcgac gaagtgctac gcctgaagca aattaacggc ctgcaagagt
     1861 tgtttgccga ccgtcaatat tcccgcgcct ggacggttaa atagctaaag ctggcgcagg
     1921 cgttcagctg ctgccagcaa cgtcgattcc ttcttggcaa aacagagacg aatcagttta
     1981 tgtgggaagg gatcggcgca aaacaccgat agcggaatcg ccgctacgcc gtgctcctgc
     2041 gtcagccact ggcaaaactc aacgtcatcc agggtagata cggcgctgta atccaccagc
     2101 aaaaagtatg taccttcgca cggtaaaatc tccagccggc tttcatttaa ggcattcacc
     2161 agaatatcgc gcttctggcg ataaaagtcc ggtaacgcaa gataatgctc aggttctgca
     2221 cgtagcatat cggcaagcgc cagctgtgcc ggggtattca ccgaaaaggt cagatactga
     2281 tgtaccttgc gaatttcggc actgatgggc gctggcgcaa cgcaataacc cactttccag
     2341 ccggtcatat gataggtctt gccaaatgaa gaaactgcca ccgcccgctc acgcagctgc
     2401 ggatgcgcct gcacgctggc atggccctgt tgtgaaaagt tgatgtgctc gtaaacttca
     2461 tcgctaatga caaaaatctc gtgcccggca attgcctgcc acaaagcggc gaaatcagcc
     2521 tgctgccaga cagttgcact ggggttatgc ggggtgttga ggatcaccag tcgggtgcgc
     2581 tcgcttaaca atgcggcaaa ttcctgccag tccacgcgaa aatgcggtgg ttgcagtgca
     2641 atacgtttca ctattccccc cgaaagcgcg atggcgggcg cgtaactgtc atagctggga
     2701 tcaaagcaga tcacttcatc accattgcgc accaatgcgg taatcgctgc atataacgct
     2761 tccgtcgccc ctgccgttac ggtgatatca ctatcggcat ccggttgata gccatacaga
     2821 cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca cgccagtcat aggcgcgtat
     2881 tggtttgccc cctggtcaac atggtacgcc agccgctcct gcaaatagcg cggaccatca
     2941 aaatcaggaa agccttgcga aaggttaatc gcctggtgtt gctgcgccag cgcgctcatc
     3001 tgggtgaaaa tagtggtgcc aagttgtgga agtttgcttt gtggaatcag agggttattt
     3061 gtcatttttt attgtgcctg acgaagttgt gttgaaggca ctataacacg atgctagtat
     3121 ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca acatgcctca
     3181 caatcctatc cgcgtggtcg tcggcccggc taactacttt tcacatcccg gcagtctcaa
     3241 tcacctgcac gattttttca ctgatgaaca actttctcgc gcggtgtgga tctacggcga
     3301 acgcgccatt gctgcggcgc aaacaaaact tccgccagcg tttgaactgc caggggcaaa
     3361 gcatattttg tttcgcggcc attgcagcga aagcgatgta caacaactgg cggctgagtc
     3421 cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactgctcg acaccgcgaa
     3481 agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt ccgacgatcg ccgccacctg
     3541 cgccgcctgg acaccgctct ccgtctggta taacgatgcc ggacaggcgc tgcattatga
     3601 gattttcgac gacgccaatt ttatggtgct ggtggagcca gagattatcc tcaatgcgcc
     3661 gcaagaatat ctgctggcgg ggatcggtga cacgctggcg aaatggtatg aagcggtggt
     3721 gctggccccg caaccagaaa cgttgccgct aaccgtgcga ctggggatca ataatgcgca
     3781 ggccattcgc gacgtcttgt taaacagtag cgaacaggcg cttgccgatc agcaaaatca
     3841 ccagttaacg caatcatttt gcgatgtggt ggatgcgatt attgccggag gtggaatggt
     3901 cggtggtctg ggcgatcgtt ttacgcgtgt ggcagcagct catgccgtgc ataacggtct
     3961 gaccgtgctg ccgcaaaccg agaagtttct ccacggcacc aaagtcgcct acggaattct
     4021 ggtgcaaagc gccttgctgg gtcaggatga tgtgctggcg caattaactg gagcgtatca
     4081 gcgttttcat ctgccgacta cgctggcgga gctggaagtg gatatcaata atcaggcgga
     4141 gatcgacaaa gtgattgccc acaccttgcg tccggtggaa tccattcatt acctgccggt
     4201 cacgctgaca ccggatacgt tgcgtgcagc gttcgaaaaa gtggaatcgt ttaaagcctg
     4261 acgtacatat ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg tcctgccgct
     4321 cacggaaaaa ctcctcgtag gtcatcggcg tttgatcggg atggttaacc cgcatatgtt
     4381 cgacatagtt gtcgtaatca ggcataccaa tcatcagttt cgccgcctga cctaaatatt
     4441 ttccggcttt tgccagtgaa tcaaacatca atacctccgg taaacaggtt gctgatagag
     4501 tgcattaata gctatcaacg cgctcggtca gtcccctcgc cccaacgggg agagggttag
     4561 ggtgaggggg aacactcggc accatgccaa ctttcgccaa cattcccccc tcactctgac
     4621 tttagtgcgc gcctttcgcc tgcgccacga tctcctcgac attttccggc attggctcat
     4681 acggcgtttc tttcgccgtt ggcttcggct ctttcaatgc cgccagtgcc gtcttaatcg
     4741 agaacagtgc cagaaccacc acgaccacca taaagaagat ggttaacccg gcatcaagac
     4801 ggttgttgaa caccagttgc gccagttgtg actcagtata ctgcgatgga atattgccgc
     4861 tgtcgatcat tgcctggaac ttgttagcaa tcgccaggaa gccgactttc gcatccgggc
     4921 taaacgcttt ctgccagcct gcggtcaggg tacaaatcag cagccaggcc gttggtacca
     4981 gcgccaccca ggcgtaacgt tgacgtttca ttttgaacag caccacagca cagagcatca
     5041 gcgccatccc tgccagcatc tggttggcaa taccaaacag cggccacaag gtgttaatac
     5101 cgcccagcgg atcgaccaca ccctgatgga ggaagtagcc ccacgccagt acgcacagcg
     5161 ccgttgccag caggttagca ggcagtgaat cggtccgttt caggcccgga gacaccacgc
     5221 ccagcagatc ctgcaacata aagcgcgcgg cacgcgtacc tgcatccacc gccgtcagaa
     5281 taaacagtgc ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcatgccgc
     5341 ccagcgcacc gtgcagaatg taggccatcc ccaccgccag ggtcggcgca ccgcccgcac
     5401 gggaaatgat cgactgttca cccacttcgc tggcaatctg gtttagcgta tccggggtaa
     5461 tcgcaaagcc ccagctactc accacctgcg cggcagaagc aaccacatcc gccgtccctg
     5521 ccggagccag caccgccatc gggctgttca tggcgaagta cacacccgga tcgatgatac
     5581 aggcggaaac cagcgccata atcgctacga aggattccat taacatcccg ccgtagccga
     5641 taaaacacgc ctgcccttcg ttcgccagca tcttcggcgt ggtaccggaa gagatcagcg
     5701 catggaagcc agacaccgcg ccacaagcga tggtgataaa caggaacggg aacaggttac
     5761 cggtccatac cgggcctgtg ccatcgacaa atttagttag cgcaggcatg gtcagcgtcg
     5821 ggcgcataat caaaatgcct accgccagac cgacgatagt cccgattttc aggaaggttg
     5881 agaggtagtc gcgcggagcc agcagcaacc acactggcag caccgccgcc acgaagccgt
     5941 aacccaccag catccaggtc agctgcacac ccgtaaaatc aaagtacggt gcccaggtcg
     6001 gactttctgc cacccagccg ccagagataa tggcgaaaat caggaatacc aggccaatca
     6061 ccgacacttc gccaatacgc cccggacgca gataacgcag gtagatcccc ataaacagcg
     6121 ccagcggaat ggtgaacgca acggtatacg ttccccacgg actatgggtc agggctttca
     6181 ccacgatcat cgccagtact gcaaggataa tgaccatgat cataaagcag gccaccagcg
     6241 caatcacccc ggcggttggc cccatctctt ctttgaccag ctcacccagc gagcgaccat
     6301 cgcggcgcgt agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc
     6361 cagcgagcag ccagatcatc cctggcaggt aacccatttg cgccgccagt accggcccaa
     6421 ccaacggacc tgctccggca atggccgcaa aatggtgacc gaacagcact tttttgtccg
     6481 tcggcacata gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa
     6541 ccgccagcac atttttggcg atatacagcc cataaaaacg gtaagcgatc agatagatac
     6601 agaccgacgc caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt
     6661 atcccagagc aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt
     6721 tgttcatagt tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca
     6781 tctttgtaca atttaacaac acggcaacca tgtaaatcgg agttgaaacc aatatttaac
     6841 ttaacaatgt taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct
     6901 gtcgggctca tcccaaaact gccgtaccca gccgacaagt gcaacaacgc cgcccctgtt
     6961 catcgaaaac gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga
     7021 caccgcgcac cttgccttca gacaccgggc gatgatgtgt ggcgttaagt tccgtgccca
     7081 ccacacactg tccgtcgcgg gtcatcataa atccggccat cgatcccagc gtttccgcca
     7141 gcgccgccga cgcgccgcca tgcagcaggc caaacggctg atgagtacgg ttatcaaccg
     7201 gcatttcggc ttccagcaca tcatcgccca gacgggtata cacaattccc agatgcgcca
     7261 ccattgtgtt atcgctggtg gcgttcagct cgtcgagcgt taaatggcgt ttccagatca
     7321 tgcttatgcc cccagcgtcg agccgccatc caccacaata tcctgtaggg tgatatggct
     7381 ggcgaggtca gaggcgagga acaaaatcgt attggcgatc tcctgtggac gggcgatttt
     7441 ccccagcgga atgccgagtt taaactgctc gccaaagccg cgaatacgct gctcttcggc
     7501 gtcatcgctc acccacagcg tgcgttgcat atcggtgtcg gtggagcctg gcgaaaccac
     7561 attacagcgc acgccgctac ccgccagttc cagcccaacg ctcaacgcca ggcttttcag
     7621 cgccgctttc gacgcgccat aagcactcat accaatgcgc ggcgtgtgtg cggcgtcgga
     7681 cgcaacagta acaatcgccc cgccccgctg acggcgaaac tggttcatgg tctgctggaa
     7741 caggttaaac gcaccaccga cattgaccgc aaaagtctgc tgccagtcct ccttactgag
     7801 ctgatcggtc gcgcccatgc gcaaaattcc cgccgcattg accagcacgt ccagtcgctc
     7861 cgtttcagct aacagtcgct gacatacctg cgccacctgc gcagcatcgg caacatccat
     7921 cacttctgtc gcaaagggat attgctcctg agcgaacgct tgatcaaaac cagtgacttt
     7981 cgctcccgcc tcaacaaacg ccagcgccgt ggcgtagccg atacctttcc ctgcgccagt
     8041 tacccagaca tttttaccgc tgaaatccat tatttcacct cgcgggaaag tagcttccac
     8101 caggcgtcga tggtcgggtt tttcgccagc atgacaaagt cgatatcgcc atgcactttg
     8161 cgccagcgcg ccgccagcgc catcatccgc accgaatcca gaccgtagtc gttcaggttg
     8221 tcgtcatcga acggttcatc ggactcgtcc agcaacggca ggatcacctc acgcagcgcc
     8281 gctttgctgg cggggatagg tgctggcagt aattcttcgg tcatcaccac ccggccagaa
     8341 cgtccggcca catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca
     8401 tctgccacca taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag
     8461 ccaatgtggg catatacccc ggtaataatc agctggttac gtccactctc tttcagcatt
     8521 tgctccagcg gggaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg
     8581 gcatctggcg tcaggctatc caccaccttt tgctgttccg gcgaacgggt caggccaggc
     8641 ccccacatat cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg
     8701 taataaaccg ggatattgtg ctgtttgcag tagtcgcgta gtgccgcaat attcgcgatc
     8761 acctgctcca tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca
     8821 tggattaaca acgcggcacg ttgcggttca aaggcccagt cgactttatt ctgcggaata
     8881 tcgtgagact ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct
     8941 tcaggctgat gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac
     9001 cgccgtcagc ggaagtgaat ccacacactc cacgcgatcc ggtaatttaa attcggcaat
     9061 accctgttca cgcaggaaac gacgcacctg caccgcgcgt agaggctctt ttaccaccag
     9121 ataagcacag cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcgtagat
     9181 caccgccgga tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc
     9241 acggttgatc tgatctttct cgcgcccctg cacggtgatg tacccctctg gatcaataga
     9301 gatcagatcg ccagaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg
     9361 gcttttgtaa tagccacgga aggtgtacgg accgcgcgtc atcaggcgtc caacttctcc
     9421 ttgcggtagt ggatttcctt cagcatcggc aacccacact tcatcgtccg gacacattgg
     9481 atagccctgg gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag
     9541 cccttccgcc atgccgaata cctgctgtaa ctggcagcca atctctgcgg gaatacgcgc
     9601 cgcaagcgtg gcagaaagac gtgcaccacc gacctgtaac agtttcagcg aggcaagctg
     9661 cgcccggctt tcgccttcgg tcagcgcctg taaccacagg ctgactgcgg gtggcaccag
     9721 cgcggtgacg ttcacctgat gtttttcaat caatgggaag cagagcgtgg cgctggggtc
     9781 ggcagccaga acgacagtgc cgccagcgag aaatacgccc agcgatcctg gcgaactcat
     9841 ggcgtagtta tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca
     9901 aatctcgacg ctacgacgca cgctgtagta gtagtcatta tgagtgcgcg ggatcagctt
     9961 cggtgtgccg gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgacgg
    10021 cgtggcagta aaatcctcag ccggatggtt aatcgcatcc tgcaagttat gctcaccgct
    10081 gtcgttgtgc agttgcacca cgcgaatgga ggaatgctct gcaacaaatg tattgaggaa
    10141 atcatctccg ctaaacagcg catgctggcg atcggcaatc agtaacgccg gttcaatctg
    10201 gctggcatag gcgttcagtt cactacgctg atggctgaac agcgccagca ccggcgcaac
    10261 gcccagtttc agcagcgcga aaaaggtaat gtaaagttca gcgacgttac ccagttgtac
    10321 cagcgcggtt tcaccaggtt taatgccctg acggcgcaaa ctacacgcga ggttatccgc
    10381 cgcctgattc agctcccggt aactcaactg ccgctcgccg tcgatgaccg cgatgctgtc
    10441 gcttgcggca tgacgggtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc
    10501 ccgatagcga cgggaaaact cttccggcca gcgggtgaat ggaatgctca tcctcactcc
    10561 ttaatgcaat ccaaaaacgt tcaacatggt agaaagtttg acgcccgttt cgcgccactc
    10621 acccaacggt gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc
    10681 ccgcagcttc gcgcagcgga tggtcaccac ccactcgccg ttaccttcgc tgtcacacca
    10741 acccacaatg ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgggt
    10801 cgcggcctga tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagcgt
    10861 cagtgcgttc tcatgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt
    10921 cggcgtggtg atcagttgtg gagaagaagg gacgtgtaac tcactactgc gttcgcgcag
    10981 tacctctttc atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag
    11041 cagacgatta cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc
    11101 taacggaatg gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc
    11161 cagcagaacg ccaccatcag ccagcgggat atggaagttg taactaaccg ggttttgcgc
    11221 aatcaaccgt tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa
    11281 ccgtgacaac accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgagcaac
    11341 catctgttca aacgtggttt gatccggaat tgcctggcgt tccaccacgt tcagcgactg
    11401 gctgcgggta aaacggcggg ctgaagattg tttttcctgg cgggagaacg actgccagga
    11461 ttcaggaata tacagcgatg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac
    11521 cggatttttg atgccctgcg ctttggcatc ggcaaacagc tcggcgagtt tttgctggaa
    11581 gggactgtcg ggtgaatccc cgttcacagc gggttcatcg aagcgggcga aacatcctga
    11641 cgtcgtaaaa ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat
    11701 ggtctgctgt acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag
    11761 gctttaataa tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta
    11821 tgtcaactct tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat
    11881 gaaaatgaga agcattaact tcattaataa caggaagctg acttgtgaga ctcgccccgc
    11941 tctaccgcaa cgcccttcta ttaacaggac ttttgctttc aggaatagtc gcagttcagg
    12001 ccgccgactg gccgcgtcag attactgaca gccggggcac tcataccctg gaaagccagc
    12061 cgcagcgtat tgtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc
    12121 cggtgattgc cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt
    12181 tacgccagtg gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac
    12241 caagtgcaga agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaaccggcg
    12301 gcgattcggc gctggcgctg tacgatcagc tttccaccat cgccccaaca ttaatcatca
    12361 attacgacga caaaagctgg cagtcgctgt taacgcagct tggcgaaatt accgggcatg
    12421 agaaacaagc ggcagagcgg attgcgcagt ttgataagca actggcggcg gcgaaagagc
    12481 aaatcaaatt acctccgcag ccggtcactg ccattgtcta taccgccgct gcacacagtg
    12541 ctaatctctg gacgcctgaa tcagcacaag ggcagatgct ggaacaactc ggctttacgc
    12601 tggcgaagtt gccagcaggg ttaaacgcca gccaaagcca gggcaaacgc catgacatca
    12661 ttcagcttgg tggggaaaat ctggcttcag ggttaaatgg cgagtcacta ttcctgttcg
    12721 ccggtgatca gaaagatgcc gatgcgattt atgctaatcc gctgctcgcg cacctgcctg
    12781 cagtacaaaa caagcaggtt tatgcgctgg gaaccgagac gttccgtctg gattactaca
    12841 gcgccatgca agtgctggaa cggcttaagg cgctgtttta agcattaact gtcggacgct
    12901 gtcacctgcg gcggcgtctg gcgaaaacgc cgcaactcca ccagcaccag caataacaac
    12961 acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg aagcaaccgg tgtcatcatc
    13021 gcgcccaaac cgcccagcag cgccgcgcct atggcatcgc ccgtcacgtt ttgcgccgtc
    13081 cacaaaccgt taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtgtat
    13141 tgcagcaacg aactaaccgc gctcagccag ccgaacagcg ccagacaaac cacgcctaaa
    13201 atccacatcg gcatcaggcc aaacagacca atggcgagga acgatcccag cgtggaaagc
    13261 agcatcaata accctggtcg cgcgctatgt gccagcttcc cgctggttaa cgcgccaata
    13321 gccgcaccga gcgggatcgc cgcatagaga aaaccaatct gtgccgccga catctgccag
    13381 ttgtcagcca gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc
    13441 agcagcgcaa tccctcccac cagcgggctg gcgagcagaa aacgaaatcc tgccagtaat
    13501 gatttcaacg gatgctcacg cggctgcggt ggcggtggca acgccggaag gcttaacaac
    13561 ggtagcaagg taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccgccg
    13621 gtcgccagca ataaaccgcc aatcataggc gaaatcaccg accccagacg cacggtcaac
    13681 atggtgatcg ccccggcctg cattaagttt tcacgcccta ccagtgcagg tgtcgccgcc
    13741 agtagcgccg taacgccaag cgatgcgaaa aaaccatccc ataaaccgag taaatagatt
    13801 gccaatagag atggctccgg cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca
    13861 caggtgccgc gcgccagcaa aatcactttt ttgcgttcat agcgatccgc cagcacaccg
    13921 ccgaccatca ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc
    13981 caggtagaat gcgtcatcat ctggatctgc accggtaccg cgacgccgag caaacccaga
    14041 gacacaattg agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac
    14101 aggctgaggt taagcagcca ggattgttta ttcattacaa taccttgcca tcaaatattt
    14161 taaataccta ttcttaggct gcacatgcta acatatccaa atatgatcga taacgataat
    14221 taatttcatt atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc
    14281 cgtgcccgga ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc
    14341 aaaatcactc cccgcttccg tagtgctgga ggccttctcc ggcacctgcc agagcgccga
    14401 ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt gccggtttac tggccggcgg
    14461 tgcgcttggc cttgccgggg cgttgatgca aaccctcacc cgaaacccac ttgccgaccc
    14521 cggcttgctt ggtgtgaacg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt
    14581 tggttactca tccgcgcagg aacaactggc gatggccttc gccggggcgc tggtggcctc
    14641 attgattgtt gcctttaccg gcagccaggg cggcgggcag ttaagtccgg tgcgtttaac
    14701 cctggcgggc gtggcactgg cggcggtgct ggaagggctg accagcggca tcgccctgct
    14761 taatcctgac gtctacgatc agttgcgttt ctggcaagcc ggttcgctgg atattcgcaa
    14821 tctacatacc ttaaaagtgg tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt
    14881 actgagccgc gcgctgaaca gtttaagcct cggcagtgac accgcgacgg cgctgggtag
    14941 tcgcgtggcg cgcacacagt tgattggtct gctggcgatt accgtgcttt gtggtagtgc
    15001 gacggcggta gttggcccga ttgcctttat tggcctgatg atgccgcata tggcacgttg
    15061 gttggttgga gcagatcatc gctggtcgct gcccgtcaca ctacttgcca cccctgccct
    15121 gctgctgttt gccgatatca tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt
    15181 agtcagtgcc tttattggcg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg
    15241 tggcgcatga tttacgtctc tcgccgatta atcatcacct gtttgctgct aattaccgcc
    15301 tgtgtggttg caggtatctg gggattacgc agcggtaccg tcacgctgga aacctcgcag
    15361 atattcgccg cgctgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg
    15421 cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg cactgggcgt cagcggcgcg
    15481 atttttcagt cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc
    15541 ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg gtcaggacct gacggctatt
    15601 gccctggcag caatggcggg gggcattatc acttcgctgt tggtctggct gcttgcctgg
    15661 cgtaacggca tcgacacctt tcggttgatt attatcggca tcggcgttcg cgccatgctg
    15721 gtggccttta atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcgggt
    15781 ttgtggaatg ccggatcgct caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc
    15841 atcattatat tgatgctcat tgccgccgcc ttactggtac gccggatgcg cttgctggaa
    15901 atgggcgatg ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg
    15961 atgctggttg cagtggtgct taccgctgcg gcaacagcgc ttgccgggcc gatttccttt
    16021 attgctttag tcgcgccgca cattgcccga cgcattagcg gcaccgctcg ctgggggctg
    16081 acccaggcgg cgctgtgcgg ggcgttgtta ctgctggcgg ccgatctctg cgcccaacaa
    16141 ctgtttatgc cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac
    16201 cttatcgtct tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc
    16261 gtggcgaaca gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag
    16321 aaatacctga tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccacgt
    16381 tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggtg
    16441 agcacattca acattacgcc agtaaagagg ttgcacgccg gattggcctg ttggcgcaaa
    16501 acgccaccac gccgggagat atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc
    16561 atcaaccgct gtttacccgc tggcgcaaag aagatgaaga ggccgtcaca aaagcgatgc
    16621 aggccacggg aatcacccat ctggcggatc aaagcgtgga taccctctcc ggtgggcaac
    16681 gccagagagc gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgatg
    16741 aacccacgac ctggctggat atcagtcatc agattgatct cctggaactg ttaagcgaac
    16801 tgaaccgcga gaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc
    16861 gttacgccag ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga
    16921 aagagattgt cactgctgaa ttgatcgagc gcatttatgg tctgcgctgc atgattattg
    16981 acgacccggt tgccggaacg ccgctcgtgg tgccgctcgg acgaacggca ccttcaaccg
    17041 caaatagtta aactaagtgc tcttacatca cggcatcctg ttttctggat gccatcgcat
    17101 ggcgcaacag cacgccacca caagccacca tcccgccgat catcgcagaa aggatcacaa
    17161 taatcgcctt acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa
    17221 acggcgtaaa attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactgac
    17281 gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac cgctttttca atttccagtt
    17341 tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc ggggtcatct ttaacggcct
    17401 gaccattact gtaaacgggc tttttaattc ctgccgcgtt agcaatgtcg agtgaataat
    17461 tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt aatgcggtcc tgagccagtt
    17521 tttctttttc aaactgggtt ttgatctcca gtttattacg gacgttttct atcgactctt
    17581 tcaccaccaa cgcagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac
    17641 tggttggcgc ggtaaaactt agcgtccagg agttatacag tgacggttca tcttttttct
    17701 tactggcatt gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca
    17761 aatccagctc gtcgattttc gcctctttta attggtccat cacataaggt gttgagcgca
    17821 ggtactcttc cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg
    17881 tacgatcaat tttgatatcc agatccagca cacgaagctt agtgaatgtt ttctcaagcg
    17941 cctgccactg aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca
    18001 ggatgaaaga gatcagcaat cctgtgcagg caaacgcaaa aacgaccgcc atgaccgttt
    18061 ttttggcccg ccataaaact tcgattagat taagtaaatc aatttcatta ttactgggcg
    18121 acgccagagg atactcggga aaatgagtgt cacttccctg tttaatattc agtgatgaca
    18181 tggggtataa cctgaaaatg agtccaataa gagtaaatct ccacggcccc cactctacca
    18241 gttggtgaga tttatccgaa gctgaagttt gtaaattact cccactgaat gcaacattca
    18301 ctctatttca gagaaacacc caccaaattt ataaattaaa caaatgataa acccataatt
    18361 attttctgta attatgggtt tatagataat ataaatttac ttatttagcg tcgcacgaat
    18421 aatcggccca attttttcaa acgccaccgg agagataata tccacatgcg cacaatcctg
    18481 acgatagata tccagctccg caatccacgg cgaccaggcg cgttcgggat tcataccttc
    18541 ctgaagcgtg cgttcagcaa caaacagcgt cgctttaccg tcaaacggta cactatgagc
    18601 agtcgtcagc aggcgcacag catcagcgta gttgccttca atggtggtaa acaactccgt
    18661 tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc tcgcggttaa tctccgccag
    18721 tacttccggg tccagaccat tagcctcttt tttctgccag ttttgcgttt ctggcggcca
    18781 ggtatccagc aagccaagaa atgccacctg ttcaccacgg gtacgcagtc gcgccgctat
    18841 gccctgcgcc agcgtaccgc ccagcgaata ccccagcagg taataagggc cgtgcggttg
    18901 ttgttcaagt aacgttgcca gatgcgcttc gcagacttca tccaggtttg ccgcggtctg
    18961 catggggcca tgagggcgcg gcgactgaat gccgataatc gaccattgtg gatcgagata
    19021 acgcgagagc acgctgaact gccaggcaaa accggacgca ggatggaaac aaaacagcgt
    19081 cgggccatta ccttcacgca acggcagaat ggtttcgaat cccatgcgcc gggagctgtc
    19141 ttcttcacca tcaataatcg ttgccagttt ggcgacagtt gacgcgacca tcacctgccc
    19201 cggcgtcacc tgacgggcaa actgccgact taactgcgca gccagtttca ttgccagtag
    19261 cgaatgaccg ccaagcgcaa agaaatcagc atcggcatcc tgcacgtcac aacccagcaa
    19321 cgacgagaat gccgcggcga taatcgtttc actgcccgct ttcggcgcac gccccggcgc
    19381 ttgtgctttc agttcaggca acggtaaggc tttgcgatcc agcttgccgt tggcgctaag
    19441 aggtaactgt ggaagttgca gcagaacgac gggtaccata tgcggcggca atgtttcgcg
    19501 cagttgcgcc tgcaatgcgc tggtatccaa cggcagacct gattgcgaca ccagatagcc
    19561 caccagctga cgcgcatccc ccccggtggc tgtcgcctga ttaatcacac aagcgtgggt
    19621 aacagcttgc tcgacatccg gcagcgcctg catcacgcga tcgatttcgc ccagttcaat
    19681 tcgctgacca cgaattttta gctgatcgtc actacgcccg agatactcca ccgcgccgtt
    19741 ttccagccag cgggcaacat ctccggtacg gtacatgcgt tcacctgggg taaaaggatc
    19801 ggcaataaag cggctggcgg tcagatcggg tcgtcccaga tacccttgcg ccagttggat
    19861 gccggtgaga tagagatcgc ctgccacacc cggcggcacc ggatgcaaca tcgcatcaag
    19921 aatacgcagg cccgtattcc acactggata accaatcggc acgctgctac cgcgcacctg
    19981 tgccagttcc tcgccaaaag ccggatacca gctgacatct accgccgctt ccgtcgggcc
    20041 atatagatta tgcaacggca cgccggttaa ctgttgccat tcgcggcata aatcggccgg
    20101 taaggcttca ccgctacaaa aaacctgttt caaggtcgcg caactctggc gagcagtttc
    20161 cggcgtcagc gaagcaacaa atgccgccag catagagggc acaaagtgcg tggtcgttac
    20221 gccatattcg gcaaagaatt gctgcatagc gagcgggtcg cggtgcgctt ccggttcagc
    20281 catcaccagt ttcgccccgg caataaacgg ccagaaaaac tcccacaccg agacatcaaa
    20341 actgcacggc gttttttggg caacgacatc ttcgcctgta agcgggtaat ggttttgcat
    20401 ccacaacagg cggttgacga tagccgtctg cccgaccatt acccctttcg gtctgccggt
    20461 ggaaccggag gtaaagatga tataagccgt gtggtgcggt tgtgaaagtt gcagcggcgc
    20521 actgccctgc ggtttaagcg gggcgttata gcaaaggctg gttaaatctg gaacatcggc
    20581 aaagcgcggc agttgatcgt cggtggtgat taacagcgac gggcgcgcat cttccagcat
    20641 catcttcaga cgatcgtccg gatagccggt atccagcggt agccaggccg cgcctgcttc
    20701 aacaattgca tgcagcgcca gggtcaaaaa gaccgagcgc ggtagcgcca ccgccacgct
    20761 gtcgcctggt ttaacgccgc gctcacgcag cagattcgcc agcgccacca cctgttcgcg
    20821 catttcccga tagctaaact ggtaacgcgc atcaaccagc gccggagcat ccggtgtttt
    20881 tgccgcttgt tctgccacta gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc
    20941 gttgatctgc gccagctgcg catattcacc tggcagcata atatcgacat cgccgcacaa
    21001 cagcgccgga tccgcggcga actgggcaat cagcattttc aggcgttcag cgtgttgaat
    21061 taacgttggc tcatcgtaac gctgtttatt ggcgaggatc tcaatactca aatcaccgtg
    21121 ttcatctggg aacagggcca gttcaaggtc attaaccgga ccggttgcca gggtatgggt
    21181 ttgcgtctga acaccatgaa tatccagttg gtaatcaaat accttgatat tgagtaccgg
    21241 accgaacagc ggttcctctc ctgccgctcg cccgctgtca cggacaattt gttcggcatc
    21301 gtaacgttga tgacgacgca tttttttcag ttgtgctgcc agtcgggttg ccagttccgg
    21361 cagcgtttct tgtgccgcaa tgtgaatccc caacggcaaa acgttgagca cgggtccggt
    21421 agccgtcagc gccgccgagc ccagtcgacg cataaagata aatccggcgg cgtagtccat
    21481 gcgattgcac aatcgcccca gccacaaagc cgccagcgca agggctaaat cggtacgctg
    21541 cacacctgaa agttgcatag ccagctggcg gaattccccg tcggtaaatt ccagtttcag
    21601 gcgcaaaata tctgccgaag cgctgcgccc cggtaaaggt gccggagaaa gtgacgcggg
    21661 cggtggcagt tgacgacgct gttccgccca gaatgccgca tcacgctgcc aggcttcact
    21721 ttcgcggtac tgctgatact cttccactac atcagcgaaa gacgtaaacg gcgaagcagg
    21781 cgttggttcg ccacgcagca atgcgcagta aatattggcg atctggcgtg taatggccgg
    21841 gaaactgaag ccatcgacca gcaaatggtg ataacgctga taccagtacc agcggttatc
    21901 cgccacctgt atcaactgat gaaagaccag tggtttaccg ctatcgacgc gcagatcttg
    21961 ttgcaaatcc gcctgcatta atgcccgcgc agtaccgtgc ggatcaatgt tggttcgcag
    22021 gtcgataatt tctggcagtt cgaacgtcag cgcatcatcg acccactgcc agacttcgcc
    22081 gttatcttcc gtaaaacgca tccgcagcgt atctgcttgc gctaatccgg caaccaccgc
    22141 gcgggccagt aatggcgcat caacctctcc ggttaactca acgtaatgcg ccacgctcca
    22201 ggcggagggt aaatctgaca gtttttctgc catccagatg ccgggctgtg cggcgaccaa
    22261 aggtaaatgc tggctcattg cgcctcctgc aactgggtga aattcgacgg tgtcagagta
    22321 cgccagtggg cttccagcca ctgctggcag gacgcctgtg actgcggctg acacacaacg
    22381 tcccagcctg ccggtaaggc gcattgttgc ggccacagac tgaattgccc ctgcgcattg
    22441 cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat tactgaatgc catattcaac
    22501 tcctgtcatg gaaaagtggt tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc
    22561 aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa aatggattct tttatagggt
    22621 gtaattgcgc atacagcgcc tgattggccc gcatgatcat cggctcgcga atacccgctt
    22681 ccagcacaat gcgcagacct tcggcgctaa cttcaccagc tttaagttgc tcaagtaaaa
    22741 caccttcttg ctgcccgccc cgatgcggcc accagtacga tcctgactgg cttaatacac
    22801 agccaaaacg ttcaggccag tgcagtccgg catacagcgc ggaaagccca ccaaaactct
    22861 gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg ggcaatagct ttcaccaggg
    22921 gtaataactc ttgctgtact gccagccaga aatccgcatt acacggcagt tcgtgagcgc
    22981 ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc gggaggaagt tgccgacgat
    23041 gggtcagcga agtcagcgca ggccagacgg gcatactctg cgcccaaaat tcgccatcaa
    23101 gcaaaactgc cagcgggcgt tcttctgctg ttgcatcgcc ggtggtaaaa atccatacac
    23161 ggcgtgaatt tttcaactgt tcacttttcc agataatttc tttgacaggt atttctggcg
    23221 cttgcggaca atcccatccc ggttgcagag gcgcttgcgg catttcgagt gcagaaacag
    23281 cgtgccctcg cccgcctttc cagctttgtg gattcagcgg atcggctatc gcctggggta
    23341 atagttttcg ccagccttca cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt
    23401 catcgcgttc ggtgggaata aaacagtagc tgccgcgcca gttggcattg agttgtgtcg
    23461 tccactgcca gacgttggtg cctgcaattc gctgcatcga ctggggctgg ctgttctgat
    23521 ggtgatcggt cacaccagtg acgtagaccc atacccgctt tatcgtcgag tattcttcag
    23581 aaccttgggg atcacgccac cagaaagtga cctcaaacat ttcgtcattc agacgctgcc
    23641 attccgggcc atgtttcgac tgccaccagc tctcacttcc tacttttaac gccgtcacac
    23701 cataacccca tgtttactgt gcaatttttc attgattgca gaaatatatt gataatatta
    23761 ttgataacta tttgtatttg caatagcgta atggcgcgcc gtgggaagcg cggacattaa
    23821 ttaaccaact gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc ggggtgcgtc
    23881 gtattgatga cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca
    23941 atgaacaaga agattcattc cctggccttg ttggtcaatc tggggattta tggggtagca
    24001 caggcacaag agccaaccga tactcctgtt tcacacgacg ataccattgt cgttaccgcc
    24061 gccgagcaga acttgcaagc gcctggcgtt tcgaccatca ccgcagatga aatccgcaaa
    24121 aaccccgttg cccgcgatgt gtcggagatc atccgtacca tgccaggcgt taacctgacc
    24181 ggtaactcca ccagtggtca gcgtgggaat aaccgacaaa ttgatattcg cggcatgggt
    24241 ccggaaaaca cgctgatttt gattgacggc aagccggtaa gcagccgtaa ctcggtgcgt
    24301 cagggctggc gtggcgagcg cgatacccgt ggtgatacct cctgggtgcc acctgaaatg
    24361 attgaacgta ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg
    24421 ggcggcgtgg ttaacatcat taccaaaaaa ggcagtggtg agtggcacgg ctcctgggac
    24481 gcatatttca atgcgccaga acataaagag gaaggtgcca ccaaacgcac taactttagc
    24541 ctcaccggtc cgctgggcga cgaattcagc ttccgcttgt atggcaacct cgacaaaacc
    24601 caggctgacg cgtgggatat caaccagggc catcagtccg cgcgtgccgg aacctatgcc
    24661 acgacgttac cagccgggcg cgaaggggtg atcaacaaag atattaatgg cgtggtgcgc
    24721 tgggacttcg caccattgca atcgctggaa ctggaagcgg gttacagccg ccagggtaac
    24781 ctgtatgcgg gcgataccca gaacaccaac tctgactcct atacccgctc gaaatatggc
    24841 gatgaaacca accgtctgta tcgccagaac tactcgctga cctggaacgg cggctgggat
    24901 aacggcgtga ccaccagcaa ctgggtgcag tacgaacaca cccgtaactc gcgcatcccg
    24961 gaaggtctgg cgggcggcac agaagggaaa tttaacgaaa aagcgacaca ggatttcgtc
    25021 gatatcgatc tcgatgacgt gatgctgcat agcgaagtta acctgccgat tgatttcctc
    25081 gttaaccaga cgctgacgct gggtacggag tggaatcagc aacggatgaa ggacttaagt
    25141 tccaacaccc aggcgctcac cgggacgaat accggtggcg ctattgatgg cgtgagtgcc
    25201 accgaccgta gcccatattc aaaagcagaa attttctcgc tgtttgccga aaacaatatg
    25261 gagctgactg acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagcattgtt
    25321 ggcaataact ggagtccggc gctgaatatc tcgcaaggtt taggcgatga cttcacgctg
    25381 aaaatgggca ttgcccgtgc ttataaagcg ccgagtctgt accagactaa cccaaactac
    25441 attctctaca gtaaaggtca gggctgctat gccagcgcgg gcggctgcta tctgcaaggt
    25501 aatgatgacc tgaaagcgga aaccagtatc aacaaggaga ttggtctgga gttcaaacgc
    25561 gacggctggc tggcgggcgt cacctggttc cgtaacgatt atcgcaataa gattgaagcg
    25621 ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg ggataacgta
    25681 ccgaaagcgg tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg
    25741 atgtggacca ataacatcac ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt
    25801 ttgtcgatca tcccggagta tacgttgaac tcaacgctga gctggcaggc acgggaagat
    25861 ttgtcgatgc aaacaacctt cacctggtac ggcaagcagc agccgaagaa gtacaactat
    25921 aaaggtcagc cagcggttgg accggaaacc aaagaaatca gcccgtacag cattgttggc
    25981 ctgagcgcga cctgggatgt aacgaagaat gtcagtctga ccggcggcgt ggacaacctg
    26041 ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca cgggcgatct ggcaggagcc
    26101 aactatatcg ccggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtatatg
    26161 agcattaata ctcacttctg atgctaacgt cagattgttg acaaagtgcg cgtcgttcat
    26221 gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat cttgccaatt caatatattg
    26281 caggaccatg taggcctgat aagcgtagcg catcaggcag ttttgcgttt gtcatcagtc
    26341 tcgaatatag tcgatatgaa aactacgcat accgccctcc cctttgccgg acatacgctg
    26401 cattttgttg agttcgatcc ggcgagtttt cgcgagcagg atttactctg gctgccacac
    26461 tacgcacaac tgcaacacgc tggacgtaaa cgtaaaacag agcatttagc tggacgaatc
    26521 gctgctgttt atgccttgcg ggaatatggc tataaatatg tgcccgctat cggcgagcta
    26581 cgccaacctg tgtggcctgc ggaggtatac ggcagtatta gccactgcgg gactacggca
    26641 ttagccgtgg tatctcgtca accgattggc attgatatcg aagagatttt ctctgtacaa
    26701 accgcaagag aattgacaga caacattatt acaccagcag aacacgagcg actcgcagaa
    26761 tgcggtttaa ccttttctct ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt
    26821 aaggcaagca agatacaggc cgctcaaggt tttctggatt atcagataat tagctggaat
    26881 aaacagcgga tcatcattcg actagaggat gagcagtttg ctgtgcactg gcagataaaa
    26941 gaaaagatag tcataacgct gtgccaacac gattaattga caacttctgg tacgattcgc
    27001 ccgcagccat c
//
LOCUS       DAYPHN010000057        26771 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000057 DAYPHN010000000
VERSION     DAYPHN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.057"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..623)
                     /locus_tag="ACG0BW_002245"
     CDS             complement(<1..623)
                     /locus_tag="ACG0BW_002245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFK7259198.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFTGNIHIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAKSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDS"
     gene            complement(846..2270)
                     /gene="patD"
                     /locus_tag="ACG0BW_002246"
     CDS             complement(846..2270)
                     /gene="patD"
                     /locus_tag="ACG0BW_002246"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFK7259199.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGEVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKLTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLDRVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(2292..3086)
                     /gene="ydcV"
                     /locus_tag="ACG0BW_002247"
     CDS             complement(2292..3086)
                     /gene="ydcV"
                     /locus_tag="ACG0BW_002247"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7259200.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILEAVTLSLKVAVLATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTIDLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     CSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(3076..4017)
                     /gene="ydcU"
                     /locus_tag="ACG0BW_002248"
     CDS             complement(3076..4017)
                     /gene="ydcU"
                     /locus_tag="ACG0BW_002248"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7259201.1"
                     /translation="MAMNVLQSPSRPGVGKISGFFWRKPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(4018..5031)
                     /gene="ydcT"
                     /locus_tag="ACG0BW_002249"
     CDS             complement(4018..5031)
                     /gene="ydcT"
                     /locus_tag="ACG0BW_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7259202.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(5049..6194)
                     /gene="ydcS"
                     /locus_tag="ACG0BW_002250"
     CDS             complement(5049..6194)
                     /gene="ydcS"
                     /locus_tag="ACG0BW_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFK7259203.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(6439..7845)
                     /gene="ydcR"
                     /locus_tag="ACG0BW_002251"
     CDS             complement(6439..7845)
                     /gene="ydcR"
                     /locus_tag="ACG0BW_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFK7259204.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDHHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPAPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(7924..8340)
                     /gene="hicB"
                     /locus_tag="ACG0BW_002252"
     CDS             complement(7924..8340)
                     /gene="hicB"
                     /locus_tag="ACG0BW_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFK7259205.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(8386..8562)
                     /gene="hicA"
                     /locus_tag="ACG0BW_002253"
     CDS             complement(8386..8562)
                     /gene="hicA"
                     /locus_tag="ACG0BW_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFK7259206.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLS"
     gene            8784..9014
                     /gene="yncJ"
                     /locus_tag="ACG0BW_002254"
     CDS             8784..9014
                     /gene="yncJ"
                     /locus_tag="ACG0BW_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFK7259207.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(9106..11067)
                     /gene="rlhA"
                     /locus_tag="ACG0BW_002255"
     CDS             complement(9106..11067)
                     /gene="rlhA"
                     /locus_tag="ACG0BW_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFK7259208.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERVDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVSEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLAGY
                     QRGSRKPVADPAPIYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(11140..11676)
                     /gene="sutR"
                     /locus_tag="ACG0BW_002256"
     CDS             complement(11140..11676)
                     /gene="sutR"
                     /locus_tag="ACG0BW_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFK7259209.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11729..12940
                     /gene="ydcO"
                     /locus_tag="ACG0BW_002257"
     CDS             11729..12940
                     /gene="ydcO"
                     /locus_tag="ACG0BW_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFK7259210.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATRAVAPRYAVIAAMIIGIVIVMAQGDVVTNDVVFNPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIAHRNR"
     gene            complement(12975..13643)
                     /gene="ydcL"
                     /locus_tag="ACG0BW_002258"
     CDS             complement(12975..13643)
                     /gene="ydcL"
                     /locus_tag="ACG0BW_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFK7259211.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(13945..14538)
                     /gene="tehB"
                     /locus_tag="ACG0BW_002259"
     CDS             complement(13945..14538)
                     /gene="tehB"
                     /locus_tag="ACG0BW_002259"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFK7259212.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYEVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(14535..15527)
                     /gene="tehA"
                     /locus_tag="ACG0BW_002260"
     CDS             complement(14535..15527)
                     /gene="tehA"
                     /locus_tag="ACG0BW_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFK7259213.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            15651..16631
                     /gene="ydcK"
                     /locus_tag="ACG0BW_002261"
     CDS             15651..16631
                     /gene="ydcK"
                     /locus_tag="ACG0BW_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFK7259214.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVCGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(16623..17162)
                     /gene="rimL"
                     /locus_tag="ACG0BW_002262"
     CDS             complement(16623..17162)
                     /gene="rimL"
                     /locus_tag="ACG0BW_002262"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFK7259215.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYALIIDSQ"
     gene            complement(17225..17449)
                     /gene="ydcH"
                     /locus_tag="ACG0BW_002263"
     CDS             complement(17225..17449)
                     /gene="ydcH"
                     /locus_tag="ACG0BW_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFK7259216.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(17589..19244)
                     /gene="opgD"
                     /locus_tag="ACG0BW_002264"
                     /gene_synonym="mdoD"
     CDS             complement(17589..19244)
                     /gene="opgD"
                     /locus_tag="ACG0BW_002264"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFK7259217.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(19469..20812)
                     /gene="ydcJ"
                     /locus_tag="ACG0BW_002265"
     CDS             complement(19469..20812)
                     /gene="ydcJ"
                     /locus_tag="ACG0BW_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFK7259218.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRRGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            21029..21952
                     /gene="ydcI"
                     /locus_tag="ACG0BW_002266"
     CDS             21029..21952
                     /gene="ydcI"
                     /locus_tag="ACG0BW_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFK7259219.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVIVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(21991..23631)
                     /gene="trg"
                     /locus_tag="ACG0BW_002267"
     CDS             complement(21991..23631)
                     /gene="trg"
                     /locus_tag="ACG0BW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFK7259220.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSARANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FIVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            24021..24170
                     /gene="hokB"
                     /locus_tag="ACG0BW_002268"
     CDS             24021..24170
                     /gene="hokB"
                     /locus_tag="ACG0BW_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFK7259221.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(24148..24216)
                     /gene="yncP"
                     /locus_tag="ACG0BW_002269"
     CDS             complement(24148..24216)
                     /gene="yncP"
                     /locus_tag="ACG0BW_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFK7259222.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(24242..24415)
                     /locus_tag="ACG0BW_002270"
     CDS             complement(24242..24415)
                     /locus_tag="ACG0BW_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259223.1"
                     /translation="MKKLALILVMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(24660..25190)
                     /gene="cybB"
                     /locus_tag="ACG0BW_002271"
     CDS             complement(24660..25190)
                     /gene="cybB"
                     /locus_tag="ACG0BW_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFK7259224.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            25379..26380
                     /gene="gap"
                     /locus_tag="ACG0BW_002272"
     CDS             25379..26380
                     /gene="gap"
                     /locus_tag="ACG0BW_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFK7259225.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWRVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKNATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(26422..>26771)
                     /locus_tag="ACG0BW_002273"
     CDS             complement(26422..>26771)
                     /locus_tag="ACG0BW_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFK7259226.1"
                     /translation="YPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEEAISMANDSD
                     YGLTSSLYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGL
                     HEYLQTQVVYLQS"
BASE COUNT         6833 a         6862 c         6298 g         6778 t
ORIGIN      
        1 gaatcaaatc taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc
       61 acatctttgg taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca
      121 cctttcagta caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt
      181 ttggtaatga atggtttcaa tgtccagcta cgttcaaact cactcttagc aaacttatca
      241 aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttcctccc
      301 attattttta catttgatgc aatactacaa aatcgaccta tatgtatatt ccctgtaaaa
      361 tgacaggtcg ggaatgaaaa agcgccgaca ctggagaaat tttttacagg cattgttgaa
      421 tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat
      481 aaatttcttt ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata
      541 caaaactcaa agtgtttttt cgtccacctg aaggtaaggg ttttattttt atcttccgtg
      601 aatttataca attgaacttt cattgttaat acttctactt ataaattatt aggttaataa
      661 cgatactcta aagccttatg cgaatcaact atgacaggat gaaaaatcac caacatacat
      721 ttagatcata ataactgcct aatttttccg ctagttatta atgtaatcat tctgatactt
      781 aatcaccatt acactcagaa cctaaaacga tagtggccgc cctataacag ccactaacat
      841 ttaacttaat gtttaaccat gacgtggcgg acgacggtgt aatcctccag cccataaagt
      901 gacatatcct tgccgtaacc agaaagtttc tgcccaccgt gcggcatttc acttaccagc
      961 atgaaatggg tattgaccca ggtacaacca tattgtagcc gtgcgctgac gcgatgcgcc
     1021 ctgcccacat ctttcgtcca taccgaggat gcaagtccgt actggctgtc atttgcccag
     1081 tttaccacct gttcttcatt gtcaaagagc gtaacactca ctaccggacc aaatacctct
     1141 ttttgcacga tggcatcgtc ctgtaatgcg ccagccagta gcgtcggcgc atagtaatag
     1201 ccattaccct tgcgcttttc accgccagtg atcactttga tgtgccctgt cgctttcgcc
     1261 tcttctactg ctttgctgac gcgatcgaga tgcgccagtg agcttaaagg tccaagctcc
     1321 gtagactcgt catccggcgc accagatttt aacgttgcca ctgcagcacc gagtttttcc
     1381 accagcgtat cgtaaatacc tttttgcgcg tagatccgac aagccgcagt acaatcctgt
     1441 ccggcattgt aatagccaaa agtacgtaca ccttcgacca ccgcttcaat atccgcatca
     1501 tcaaaaacta tcactggcgc tttgcctcca agttccatat gagtaagctt tatggacggc
     1561 gcggtatggc tgatgatgtg ttccccagtg gcgatagagc ccgtcagtga caccatccgt
     1621 actttgggat gtccggtcag cggatcgccc accgttttgc ctctgccaaa cagcacgtta
     1681 atcacgccag ctgggaagat atctttcgcc agctctgcca acttcaacgc tgtcagcggg
     1741 gtaatttctg atggtttgag cactacgcaa ttccctgccg ccagcgccgg agccagtttc
     1801 cacgcagcca tcatcagcgg ataattccac ggtgcgatcg aagccacgac ccccaacgga
     1861 tcgcgacgga tcatcgaagt atgaccttca agatattcac ctgccgccag accattcagg
     1921 cagcgagctg ctccggcgaa aaagcgaaag acatcgacaa tcgccggaat ttcatcattg
     1981 aacgcactat gcagcggttt gccacagtta cgggactcca gttcggcaaa aacttgacca
     2041 ttttcttcga ttacatccgc cagtttcagc aggcattccg cacgcgcttt cggcgtggtt
     2101 tgcccccatt cggcaaatgc tgcatctgct gcacgaacag cggcatcgac ctgctctgca
     2161 gatgcctcgg caatctccag taaaacttcc cccgttgccg gattatagac aggctgtttc
     2221 tccccttcgc cgctaaccag ttctccgtta atcagtaact tatgttgcat agcatattcc
     2281 tgtatcagtg gttattgacc attgtcgcct tcgcgggtta gccaccaggc tcccaggatc
     2341 ggcaaggttg ttaccaacat aaccagcagt gccaccacgt tggttaccgg tacatcacgc
     2401 ggtcgcccaa gctgattgag caaccacaac ggtaacgttc gttcatgacc tgccgtaaag
     2461 gtcgtcacga tgatttcatc gaacgacaag gcaaacgcca gcattcctcc tgccagtagc
     2521 gccgaactga gattcggcaa cactacgtag cggaaggttt gccagccatt ggccccaaga
     2581 tccattgacg cctcaaccag actgcacgag gtgcggcgaa aacgggcgat gacattgtta
     2641 aacaccacca ctacgcaaaa agtcgcatga ccgaccacga tggtgaaaaa tcccggctcc
     2701 agatcgatgg ttttaaaggc ggttaatagc gccagaccag tgacaatacc cggcagcgca
     2761 atgggcagca gcagtaacag cgaaatggcg tttttgccga aaaagtctcg tcgccacagc
     2821 gcggcagcgg ccagcgtccc taacaccagc gcaattaatg tcgccagcac cgccacttta
     2881 agtgacagtg tcacagcctc aagaatatca ctgcgctgtg ctgccacgct aaaccagcgc
     2941 agcgtcaggc cttgcggtgg aaaactaaac gccgcatctt cagtgttaaa cgcataggcg
     3001 gcgataatca gaatgggaaa atgtaggaaa accacgccgc cccaggccgc cagtttgagg
     3061 aaaaacggtg cgcgttcaga gtgcatcgaa cgctcccaga cgtttcacga acgccaggta
     3121 cagtgcgatg agaacaatcg gcaccagggt gaatgccgcc gccatcggca tattgccaat
     3181 cgctccctgc tgggaataaa ccatattgcc gataaaatat cctggagggc caaccagctg
     3241 cgggacgata aaatcgccca gtgtgagtga aaaggtaaag atagagccag cggcaatacc
     3301 cgggattgcc agcggcagta ccacatagcg aaaggtttgc cgtggacgtg cgccaagatc
     3361 agccgacgcc tgcaacaatg acggcggcaa acgctcaagc gccgcctgca cgggcaggat
     3421 catgaacggc agccagatat agagaaacac cagaaagcgc cccagcccgg aagttgacag
     3481 cgtgttaccg cccaccgctg gtaatgtaag gaacgctgtc agcagtggtt ccagccccag
     3541 atgttgtaaa aaccactgcg ccacaccatc tttcgccagc aataacgtcc aggcataggc
     3601 tttaacaatg tagctcgccc acatcggcaa cattaccgcg atataaaaaa acgctttcat
     3661 tttcccgctg gtatagcgcg ccatatacca cgccattgga aaagccagaa tggcgctggc
     3721 gatagtgact gccacagcca taatcagcgt gcggagaatg atgtcgtaat tcgccggatt
     3781 aaacagcgca cggatattcg ccagcgtcag ttccggcgtt accgacatgg tgaaatcgtc
     3841 aaaagtataa aagccctgcc ataacagcgt tagcagcgag ccgaaataga caatgccaaa
     3901 ccacattagc gggccaagca gcaacaaaaa cagccccagt cctggttttc gccagaaaaa
     3961 gccggatatc tttccaactc ctggacgtga aggtgattgc aatacgttca tcgccattca
     4021 cctctcctca accagcggca ccatcacatc acgcgaccag gaaaccatca cttgttgtcc
     4081 gggcgtgagc gtggcaggca gttcttcgcc tgtcatattg gcctgactca caagcagttt
     4141 ttcaccgccg ttcagtctca gttcaaaacg agttgccgcg ccctgatatt gcaccgcctg
     4201 gatcgtgcca ttggcctgca gttcaccagg ggtgttgagg cggatatgtt ccggtcgcag
     4261 ggcgaagctt cccgtcatgc cacaaagttt ctctgccatc agtccatcaa aaacattcga
     4321 tgtaccaacg aacccggcaa caaacggcgt gcgcgggcgc atatagagat cgcgcgggga
     4381 atcgacctgc tcaatgcgtc cattattgaa aaccgccaca cgatcggaca tcgataacgc
     4441 ttcgccctga tcgtgggtaa cgaagataaa agtgataccg agagattgtt gcagtttttt
     4501 cagttccagc tgcatctgct cacgcaattt gagatccagt gcgccgagcg gttcatccaa
     4561 cagcaatacg cgcggttcat tcaccaatgc tctggcgata gcaacccgct ggcgctgacc
     4621 accagaaagt tgtgacggtt tacgttgctg tacaaacccc aacgccactt tctccagcgc
     4681 ctcttgcgcc attgcgtgcc gctgcttttt attcacgcct ttgaccatca gcccataggc
     4741 gacattgtca agaatcgaca tatgcggaaa tagcgcgtag tcctggaaga cagtattcac
     4801 gtcccgctcc cacggtggca gattgctggc gggtttacca aagatagaga tagccccgcc
     4861 agaaagctgt tcgaagccag caatcaggcg caggcaggta gttttgccgg agccggacgg
     4921 ccccagcata gagaagaact caccatcttt tatcgcaata ctgacgccat ctactgcgcg
     4981 cacgtcaccg tacaaccgcg agacgttgtc aaactccact gcgtacgtca taaagcaccc
     5041 cagcgaagtt agcgaccgcc cataatggca atgtaatcct gcgtccagcg actgtaggga
     5101 acaaacttgc ccccttctgc tataggcgtt ttccagaagg cgattttatc gaaatagtta
     5161 aaaccgttgg tttcacaacc tttttcgcct aataacggac tggctttaca cccttccggc
     5221 actaccggta acgagccaaa ccaggccgcc acatcgccct gcacttttgg cgttaatgac
     5281 cagttcatcc atttgtaggc gcaaaccgga tgtttcgctt cactatgtag catggtggtg
     5341 tcagcccaac cggtaacgcc ctctttcggg aatacggtcg cgacaggctg gccttcggct
     5401 ttcagggcgt tggcctgata gggccaggca ctggaagcaa ccacaccctc gtttttgaaa
     5461 tcgctcattt gcacggtagt gtcatgccag tagcgatgga tcaaactatg ttgatcgcgc
     5521 agcactttca acaccgcctg atactgttct tcggtgagtt gatacggatc gctgatgccc
     5581 aactgcggct gagtggcttt aacgaacaac gcagcgtccg caatgtagat agggccatca
     5641 taagcctgaa cgcggccttt attgctcttg ccgtccggca gattctgctc aacaaaaacc
     5701 acttgccagc tatccggcgg cgtcgggaag gttttagtgt tgtacatcag caggttcggc
     5761 ccccattggt aaggtgtgcc gtaaactttg ccgccaacgt taaaccagtc gcctttaacc
     5821 acgcgcggat cgagcgcttt ccagttggga atcaaggcgg tattaatcgg ctgcacgcgt
     5881 ttgcccataa tcaggcgcag cgaggcatcg ccggatgccg taaccagatc gtaaccccct
     5941 ttggccatca gactgaccat ttcatcggaa gtcgcggcgg ttttaacatt caccgcgcag
     6001 cctgtctctt tttcgaattg cgttacccag tcgtattgtt tatcagtttg tccgcgttcg
     6061 atgtatcccg gccaggcgat aatatccagt cgcccttccg gtttatctaa attggtaggt
     6121 ggttcggcgg cgtgagcggt cattattgtc atggtgagcg cacacaggct gctgcgggca
     6181 aatgtcttgc tcataaagtc ttactcctgt cgtaattaat tgttcagccg ccgtgccgta
     6241 aaaatatctc cttgtttaac catagacggc ggctagcagg cacaccttcg cccctgagaa
     6301 aatttaatca ggttgtgagc taccgcgcat tatgccgtct gtaaggttat cttctggagt
     6361 atagacaagg agttaggaaa tatgactgct attcgaaatt catattaccc aacctgagag
     6421 gaatttaatt aaacgctatt acagccgttc ttgaataagt ttgcctaatt gtttaaccgc
     6481 ctgttcttca cgttctcccc actgccacgc ggtattaaaa cggaaaaaac gtgaccagtt
     6541 ttcaccggta gaaaacattt tacccggcgc aatactgata tgatgcgtca gtgccgccag
     6601 gcttaattcg cccgcatcta acggcgcggg gagttccagc cagagaaaat aaccgctgtc
     6661 attatgatga attttcactt ccgcaggcag ataacgcagc agtgcctgcc aggcacgttg
     6721 tttacgctcc gcaagctggc gacgcaagcg acgaagatgg gcgtcgtatc ggcgcgtgga
     6781 aaggtaatcc accagcgcaa gctgcatcgg tgagctggtg gaaagcgtac tcatcaactg
     6841 caagcgttga attttacgtg catgttttcc ggcggcgacc caaccaatac gaaaaccagg
     6901 caccagacat ttcgaaaacg aagagcaatg caaaacacca tcgtggtgat cccacgcttt
     6961 cgcaggcagc ggtttttccc gtccaaaata aagttcgctg taaacgtcat cttcaatcag
     7021 cgttacgttg tactgattga gcaacgctac cagccgcgct tttttctgcg gcgttaaggt
     7081 aaatccgagt ggattctggc tattagtcat cagccagcac gctttcaccg gatactcctg
     7141 caacgccagt tccagcgcct tgagatcaat cccttcttta acgtccgtcg ccaccgataa
     7201 cgccttcagc cgtaaccgct ccagcgcctg caacgcgccg tagaaacaag gattctcaac
     7261 tatcacccaa tcgcccggtt cagtgaccgc ttgtaaactg aggtttaatg cctctaacgc
     7321 cccggcggta atgacaattt catcaggaga aatagtgatg ccctgtaagg catagcgacg
     7381 agcaatagcc tgacgcagtt ctgcgtttcc tggcggtaag ttttcaatca cgctcatcgc
     7441 cgtggcggtt ttgcttacct gcgccagcga gcggtttagt tgttggaggg ggaaaagtcg
     7501 cgggtcggga aaggccgagg caaacggaac taccgacgga tcgcgactgg cctgcaacat
     7561 atcaaaaata taggtgttga tatcgactgc ttcatctcgt gtgactggaa tgactggcgc
     7621 tttcggcatt tttattgcct gtggcgcaac gtaataaccc gactgcggtc gcgcgataat
     7681 atatccctga ctttcgagca actgataggc atggctgaca gtcataaagc tcatgccaga
     7741 aagcgccacc tggtcacgca acgaaggcaa acgatcgcca ggttgccaga tacccgacgc
     7801 aatctgctcg cgtaattgtt ctgcaagctg ctggtatttt ttcattatcg aatcgtaatt
     7861 atgtgcagat gattcggcag tctatatcag ttgttaaaaa tgacaacttt tcgttaactg
     7921 taattaaacc atcaccagcg ataactcttt gccaagcgcc tttgccgcga gctggacggc
     7981 gtcgattttt gtcgcatggt gcaaattaaa taggcgagta atctcctgtt taggtttgcc
     8041 aattcgtctg gctaactctt gctgagtgat ttctgactgt aaaaaagcat ttaacagcaa
     8101 tactttagag gcgatactta aaggcacttc aataaagtga tcgttgttag ttaatggcga
     8161 aggcaacggg attagctcat tatcttcaaa ataaaaatca aatgcggtca gtaaagcatc
     8221 ttttgccgct tccatcgctt cagcaactgt ttcgccctgg gtcaacgctt cagggatatc
     8281 cacaaaagaa accatataac cgccttccgg cgcgggtgta agagtgacgg gataacgcat
     8341 aatttcgact gtctctcgca tgtcattaac ctttttaatt ggcgattaac tcaaaccgag
     8401 ttgcttcagg attgctttac gcaatggttc ttttatctca tcactggggt gacgcggcat
     8461 gacactgcgt cgcccttgaa acctgagttt caaatggttg ctgccattcg ccacatcgac
     8521 gccctgagat tcgagccaac gtctgaactc gctttgtttc acatcgcctc cctgccgctg
     8581 agtatatcaa caaagtaaac attagtgttt acctatgtgt gtaatttgct cgaaaatagc
     8641 actaaatcgc cgcaacggca gaatgcaatg caaaatcagg aaggaagatt ccataatttc
     8701 tttgcgctaa tttgcaaaac tgaataataa cctcaagaaa cgatcggcat catagcggca
     8761 aaaggtaaga aaaaggtggc aggatgttta cgaaggcgtt atcggttgtc ttattaacgt
     8821 gtgcgctgtt ctcaggacaa ctcatggcag ggcacaaagg acatgaattt gtgtgggtaa
     8881 agaatgtgga tcatcagctg cgtcatgaag cggacagcga tgaattgcgt gctgtggcgg
     8941 aagagtcggc ggaaggtttg cgcgagcatt tttactggca aaaatcgcgc aaaccagaag
     9001 cgggacaacg ttgagtcatt tattgccgga tgcggcgtga cgccttaccc ggccaacaat
     9061 atcatgcaga ttcaataagt tgtgagtaac cagaaaactg gcgttttacc cttttcgctt
     9121 cggcaatgtt ttcagcagct catccggact tacggaggcg acaacacttc ccggtaacgg
     9181 cattttcaga atgtgatttt taattttgcc gatgacgtgc atctcgcatg ggcggcaatc
     9241 aaactttagc gttaatactt catcgccgtt aaccagttgc attggcgtcg ccttccagct
     9301 tttgatattg cctttcgcct gtttcgggca cagattaaag gcaaagcgca gacaatgctt
     9361 agtgatcatc accgggactt cgcccttctc ttcatgtgcc tcatacgccg cgtcaatcag
     9421 ctgcacaccg tagcgatgat aaaattcacg cgctttctgg ttgtatacgt tcgcgaggaa
     9481 actcagatgc gtttgcggat aaatcggcgc aggatcagca accggtttac ggctgccccg
     9541 ctggtaaccg gcaagacgtg cagcatccag catgtcggcg gcttcacggc ggaactggtt
     9601 taacagactg tttggtacaa acagcgcacc cggcaaatta atttgcacat cgcgggcgta
     9661 atagatggtt tgccccagtt ttgccagacc atcctttaga ttgttcattg ctttttcggc
     9721 gttattggct tcgtcgaact gcccgtccag cgtatgcgtg atgctgacac cctcttcact
     9781 ggtgagggtc agaatcagtt gttcctgcca gccgcccagt tcaatgtcta ccgccacccg
     9841 acgttcgctg gaggtttttg tcagtgcctg ctgccagtta tgatcaagat tacggtttag
     9901 tgggtgatgc gggcgaattt tgtgcaactc tgctggcatt tcattgggcc agacgcggta
     9961 ctgattttct ccggttttct cgaccgtatt ggcacgaaag ccgacgactt cacgtttaat
    10021 catcacgttc aggccatcgc catttgccag tggttcggaa acggcaacat cgagatgatc
    10081 tttcgccact ttcaatactt cacccaccgg caggccgata aatttcggcg aatcgaacgc
    10141 gccaatatcg cctttacggg cattcacaaa ataatctgtg ctaccacggt ggaaagtctt
    10201 ttccgtcgat ggaacaaaga aatgttcagt acgacctgat gaagcacgcg tcagatcgac
    10261 acgttcttca ataatggcat ccagcatctg gcgataatgg gcggtgatat tcttcacgta
    10321 gctcatatct ttgtaacgcc cttcaatctt gaaggagcgt acgccagcat caatcagcgc
    10381 gccgaggttg gcagtctgat cgttatcttt catcgacagc agatgttttt cataggaaac
    10441 cacccgcccc tgatcgtctt tcaatgtgta tggcaaacgg catgcctgcg agcaatcgcc
    10501 acggttggcg ctacgccctg tttgcgcatg agaaatatag cactgacccg aataggccac
    10561 gcacagtgcc ccatgaataa agaattcaat ggtcgcgtcc gtggcctggt gaatcgcgcg
    10621 gatctgctca agattcagct ctcgcgccag cacaatctgc gtgaagccaa catcagagag
    10681 gaacttcgct ttttctactg tgcgaatatc gcactgcgta ctggcgtgca gttcaatcgg
    10741 cggaatatca agttccagaa tccccatatc ctgaacaatc agcgcgtcga cgccggtctg
    10801 gtagaggtca gtaatcagcc gttgcgcggg ttccagctca tcatcatgca aaattgtgtt
    10861 aagcgtgacg aaaatttttg caccataacg atgggcaaac ggcaccagct cggcaatatc
    10921 tttcaggcta ttactggcat tatgacgggc accaaaacca gggccgccga tataaacagc
    10981 atcggcaccg tgcaaaatag cttcgcgggc aatggcggca tcgcgtgccg gacttaacag
    11041 ttcaagacga tgagaagata cggtcacttt cttatccagt agctgaaaaa tggcggctat
    11101 tttagccaca agcgcgacgt tgcgaaatag ttttcccgct taactgcgcg ggtaatggat
    11161 gagtgaatga aaatgtacgg tttgctcccc tccattacga tagacatgcg tgacgtctgc
    11221 agcaaatcga gcgccttctc cacaattaag gctctgccat tcaccatcaa cgcacagatc
    11281 aagttgtcca tcaatgacga cgacatgttc gattaccccc ttttcatggg gcgtcgattc
    11341 gcttattgca ccgggtgcca tctgaatcga gaagtgttca aagcagagtt gtggatcata
    11401 aggaaacagc gaagtgatca ccatcgcctg ccgttgtgga tcataaactg aaggcgtagc
    11461 ggactgcggc ggagaaataa atgttgaaaa tggcacgttc aacccggtgg caattttcca
    11521 taatgtcgcg accgtcgggc tggattcatt acgctcaatc tgccctaaca tcgctttaga
    11581 aacgcccgtc gcttctgcca gtcgcgaaag actccagccg cgctgctgac gtagttgttt
    11641 aagtgtggtg gataaaaagc gagcgagatt ttccatgcga ctcctccggc aaaacggaag
    11701 tttatcactt gtgcgttata acggacaaat gctacggtac ctgtacgtta taacgcacga
    11761 ggtgactatg cgtctgtttt ctattcctcc acccacgcta ctggcggggt ttctggcggt
    11821 attaattggc tacgccagtt cagcggcaat aatctggcaa gcagcgattg tcgccggagc
    11881 caccactgca caaatctctg gctggatgac ggcgctgggg ctggcaatgg gcgtcagtac
    11941 gctggctctg acattatggt atcgcgtacc tgttctcacc gcatggtcaa cgcctggcgc
    12001 ggctttgttg gtcaccggat tacagggatt aacacttaac gaagccatcg gcgtttttat
    12061 tgtcaccaac gcgttaatag tcctctgcgg cataacggga ctctttgctc gtctgatgcg
    12121 cattattccg cactcgcttg cggcggcaat gcttgccggg attttattac gctttggttt
    12181 acaggcgttt gccagtctgg atggtcaatt tacgttgtgt ggcagtatgt tgctggtatg
    12241 gctggcaacc agggccgttg cgccgcgcta tgcggtaatt gccgcgatga ttattgggat
    12301 cgtgatagtt atggcgcaag gtgacgttgt cacaaatgat gttgtcttta accccgttct
    12361 ccccacttat attacccctg atttttcgtt tgctcacagt ctgagcgttg cactccccct
    12421 ttttctggtg acgatggcat cgcaaaacgc accgggtatc gcagcaatga aagctgccgg
    12481 gtattcggct cctgtttcgc cgttgattgt gtttactgga ttgctggcgc ttgttttttc
    12541 ccctttcggc gtttattccg tcggtattgc ggcaatcacc gcggctattt gtcaaagccc
    12601 ggaagcgcat ccggataaag atcaacgttg gctggcagct gccgttgcag gcatttttta
    12661 tttgctcgca ggtctttttg gtagtgctat taccgggatg atggctgccc tgcccgtaag
    12721 ttggatccag atgctggcag gtctggcgct gttgagtacc attggcggca gtttgtatca
    12781 ggcgctgcat aatgagcgtg agcgcgacgc ggcggtggtg gcatttctgg taacggcaag
    12841 tggattgacg ctggtcggga ttggttctgc gttctgggga ttaattgccg gaggcgtttg
    12901 ttacgtggtg ttgaatttaa tcgctcacag aaaccgataa taaaaagcgc catcttacga
    12961 tggcgcagct ggaattactt tttgttaacg tcaaacatag tggcatcggt cgccatgtca
    13021 tcaataactt gtttaatatt ttcgaaagcc attggcgtac tttcgttatt caggtcttta
    13081 ccttcgccct gacgaacgac tttgataacc ggtttattag tagctgcatc aatcagctca
    13141 ccttcaaaat agaggcgagt atccatggta cggtggcccg tcgccatttg cgtccccgcg
    13201 accactaatg cgacaggaac cacttcatag aattgcagcc cttctttgct ggtatcaaca
    13261 ccggtaatgg ccccacggaa aatcagacta cgcggcccag cggtggtaac cagtggttta
    13321 cgctgcgcaa tcgcttcttt catttcggtg ttggtataac tcaaaatttt atccagaact
    13381 ttctgcccta cctgggtaga tggtttcggt accggataat aagtgactgg gttccagacg
    13441 atgctgtcat atttgctttg atcaaaactc gggtctaccc aacgtaaaac aggtttacct
    13501 gttgctgagg ttgtttcttt taaatcagag taattgttta aaaaaccaga atatttatct
    13561 ggctgggtga ttttagaggc acaaccagac agagccaaca agccgcataa agctgcaact
    13621 ttcgcaattg atgtggtacg catgaatagt ttcctgagat gattatatta ctttctaagt
    13681 tatagcaaaa agcgccgctt cgtgttgtgg caaaaaaagt gttggtgaaa ataaattttt
    13741 cagccccttt tcagacgaca tagtcaatgc cgaaaaaggg gctaagtcaa tcatcagtag
    13801 ttaaatttca gtaagttaag ctgctaaaaa acttcgacta ctgtgattaa tttcacatcc
    13861 attcgataaa aattatgctt accgaaaaga agaacagtaa accatctcag cgggggaatt
    13921 atcacgcgcc ttgttcttag cgggtcattt tttacgtgcc agcatcgtgg cgaaacgcag
    13981 tttaatacga ttaccgttgg cgtcggtgcg gtgcagctcg ccgacgtctt cattgtattt
    14041 caccatctcc cagccttcgt aatatcgacg taattctccc tctttgaagg caaacgggaa
    14101 gccgacggta catggataat cagcggtatc catcgccgcc acaatcaggt tgtaaccgcc
    14161 aggtttagtg caacgttgca tattggcaat cagccctggg atggttttag cctcaaggaa
    14221 catcagcacc acagtcgaaa gaataaaatc gtattgccca tcaaacgtaa ggttattcag
    14281 atcaacgacc cgagtgtgta aattatccag attttcaatg gatttaatgc gctcgacgtt
    14341 ggcgatactc atggcatttt tatcccatgc gtcaacttca taaccattgg ctgccaggta
    14401 aagactgtta cgaccattgc cacagcccag atccagcgtt ttaccgggtt taaccacttt
    14461 caccgcttcc agtacttcag agtgtgtgcg ggttaattca tacttatcag taaaatagtt
    14521 ttcgtcacga atgatcattc tttgtcctca gctttcatta aaacggcgcg ctcggttctg
    14581 attaacaatt tcccctgcat cagaagcgcg aaagtacgga tgagcagtat tgcaattata
    14641 aaattggtaa agataaacag cggcaccgcc agcgtatgga aaaatccatt atcgctgcca
    14701 ctccccagat gcaaaccggt ggttgccagt gcagatacgc cgaacgagaa actccagaat
    14761 gaagcattaa atggctggga gagataccat ggcatcagac gtagcataaa cagcagttgc
    14821 agcagcccat aaccgaaaag cattttcgcc agcgtgtcac cctcgccgcc gttgacgctc
    14881 agccaggcac tacaggccac cagcgcagga gcgagctgaa tgccgagtga tgtccgcagc
    14941 gccgtgggta attctcccga actgcgcagt cgctgcaaga tcaccggttc caggcttaac
    15001 catgagaaaa cgcctgcgcc taaaaacacc agaccggcgt cggtgtagcc caatgcacca
    15061 caggccattg cgctgataaa gttgttggca actgtcggca gatacagtcc aggcgtggta
    15121 gcttcttcag gatgagatcc gcgccataat cccgcagttt gccaggcggc ataagccaac
    15181 tgaaccacga caccaaaact gaacaggcac accgccagtg ggcgaaacca cggaacaaaa
    15241 ccaatcgcca ccagcatcgt cgttgccgga aacaaactca caaaactgct catcactgga
    15301 tggcgaactt ccgccagcac gctatgcgga aagcgtatga gtcgggtaat aaatgcgcta
    15361 gtcaataatc cccagatgat catcgccaga atcactagcc catcccctaa ccagtggctg
    15421 acctgccaaa cctggctggc atagcgccag gcaaatccca tccctatcgt ccccaacaca
    15481 ataccaaagt agcctgccgg caaattgagc actttatcgc tctgcatctt cttactcatt
    15541 ttgtttaatt tataaagtat atttgaaatg cattttatgg aattttctcg agcatagcca
    15601 gagccgcaga atttgctacg gttatagtaa gtcacctgtg ggaaaacgct atgcgcaaat
    15661 atcgtctaag tgaagaacag cgagccttta gttatcagga agatggcact aaaaaaaatg
    15721 tgttattacg gcagattatc gccattagcg attttaacga tgtgatagca ggaaccgctg
    15781 gcggctggat cgatcgcgaa acggtgttag cacaggaagg aaattgctgg atttacgacc
    15841 agaatgctat tgcgttcggc ggaacggtga tttccggtaa tacgcgtatc accggcacca
    15901 gcgtgctgtg gggagaggtt tacgcaacgg ataacgtctg gatcgataat agtgaaatca
    15961 gccagggtgc atatataagt gacagtgtca ccattcatga ctcattagtt tacgggcaat
    16021 gtagaatatt tggtcacgcc ttaattgatc aacattctat gatcgtcgcc gcacaaggct
    16081 taacgcccga ccatcaactc ctgctccaaa tttatgatcg cgcaagggtt agcgcctcgc
    16141 gtattgtcca ccaggcacaa atttatggcg atgccgtcgt ccggtatgct tttattgaac
    16201 atcgcgccga agtttttgat tttgccagcg ttgaaggtaa tgaagagaac aacgtctggc
    16261 tatgcgattg cgctaaagtc tacggtcacg cgcaggtgaa agctggcata gaagaagatg
    16321 ccattccgac gattcattac agttcgcagg tggcggaata tgccattgtg gaaggtaatt
    16381 gtgtgttaaa acatcatgtc ttgattggcg gaaatgccgt ggtatgtggc ggaccgattc
    16441 tgctcgacga gcacgttgtc atacaaggtg agagccgtat tacaggtgcg gtgataattg
    16501 aaaaccatgt ggagttaacc gaccacgccg tcgttgaagc ctttgatggc gacacggttc
    16561 atgtccgtgg tccaaaagtc attaacggcg aggaacgcat tactcgaacg ccactggcag
    16621 ggttattgtg aatcgataat aagcgcgtat aagttcacat catcataggc atcattcagg
    16681 aactcagcct gtttcaggca accttcaagg ataaaaccat tacgcaaagc gacctggttg
    16741 ctttgcgcat tgtccacccg acatttgatc acgaagcgtc taagttcacc agactgggcg
    16801 taatgatgaa tcaatgcctg cagcgcctga gaaatgatcc cctgcccctg atgagattcg
    16861 tccagccagt agccgatttc agcggtttta ttcagtggtt caatacgatt aaacgagata
    16921 acgccgataa gttcatcttc tttgaaaatc atgaacattt tggcatatcc gcgttgatgc
    16981 aacattacat tgccctgcac cgtttttcgc gtgtcctctt cactttgaac aaactgcggc
    17041 cagtttagcg actgctgtaa ccaggtttta tttttacaga ttaactgata aagaggagtg
    17101 acgtgatttt ctgcaacagc atgtaattca agtgattcgc ttacttttat cgtttcagtc
    17161 atcttatctc cagtcaatcc atgcagttgt taaaaaagcc gcctggctgg cggcttagga
    17221 aagtttacac ctctttgacg ctctcctgct gcaggatttt gagcatctca tcttttaact
    17281 gtaacttttg ttttttcatg cggaccactt ccgcattgta ccctcgaccg tcggaacctt
    17341 cctttctggc aatttcatga tcaagtttat tgtgtttatc gaacaaggac ataaagcgag
    17401 gattttcgtt tttcagacgg gatattaaat ctcggtattc tggaaacata cgcactccct
    17461 gttagtggct gacaaggtgt atctgaaata cacactatca gattactcat ttaacttagg
    17521 gaaaaatgcg agcgggatcg tgtttaacca gacggcacct cccgaagaag gtgccgaaga
    17581 aaaaacgatt aactcatcac gcggtcgtca acatactgac gtttatccgg cgctggcggg
    17641 aaatactgat acagccatgt ttcactgata gcgtcgccct gacaacgcag atacatccgc
    17701 atatcgaccg gatcagtgga gtccgaagtc ggataccagt caaactgaat acgataacca
    17761 tcaatgggtt caatatagag aatttcgatt tgcttcgctt ccccactgga aagcgtaatc
    17821 accggctcaa tgccttttgg cgcggcagct ttcagatcac caccaacgaa atcgacggca
    17881 aaacgacgcg cccatttttc gggatagtgt tcacctggcg cccaaccttc cgggaaacca
    17941 cccatgccgg tacgcgtcgc cataacgcgc gctaatgggc aatgaacagg cggttgcgca
    18001 ctccagtaca gacgatactg gaatgcaaac tcatcacccg cttttacagc tttttctggc
    18061 tgccagaagc agacaatgtt atccagcgtt tcacccgtag ttgggatttc catcaggccg
    18121 atggtgccct taccccactt gttacgcggc tccacccaca gacttgggcg tttgttatac
    18181 cagcccataa tgtcctgata atgggagaaa tcacgatcca gttgcaataa accaaaccct
    18241 ttcgggttgt tgtcggtgta agcattgaac tgcaattttt gtggattgtt cagcggacga
    18301 caaatccact cgccgttacc ccgccacatg gacaaacgat cagagtcatg gatttgcgga
    18361 tgaatggtgt cgcacatccg acgttcatta gtaccgcagc tgaacatact ggtcatcggc
    18421 gcaatgccca gctgtttaat gtctttgcgc gcatacaggt gattttccac atccataatc
    18481 acctgacttt tctcgcaatg gatagtgaac ttataggcac cagtaatgct ggcgctatcg
    18541 agcaacgcat aaacggtaaa ggtggttgcc cccggtttta ccgtatcaaa ccagaaggcg
    18601 gtaaagtcgg ggaactcttc tttactgtcg gtgtaagtgt cgatcgccag gccgcgagcc
    18661 gacaaaccgt attgatatgt atcatcaacc gcgcggaaat aactcgcgcc gaggaatgat
    18721 acgacatcac ggcgcgccag ttcgggggct ttaaacacgc gaaaaccggc aaaaccgaga
    18781 tcactttgcc cttctaattg tttggtatca acacccgcat cgttgtattt gaacaactcc
    18841 gggcgaaagt gaatttcacg cgccagatgt gttgctggat ctacagaaaa catacgaacg
    18901 cggcgacgga atcccattcc catatggaag aactgagcgt ccagttgacg gttctcaacg
    18961 ttatgccaga gcgatttttc ggcgtcgtat tgaatactgt tataagcctg cggcgtcatt
    19021 gtcgccagag tgtcaggtaa cggacgcggc gcaccacgcc acgctgtttg cgctaagtcg
    19081 tgcgccattg actgtagaat ggagaagtca aaacgttggg tttgcccgtc ggcaatatca
    19141 gaatctgccg cgaatgccgc ctgagaaaaa agagaagcaa tgccgctggt accgcacacg
    19201 gcggccatag ccattgaacc tttaataaat cgtctacgat ccatacctga aagtgagtcc
    19261 ttctggtgtt gtgaatggtc ccgcgctggt cgttttcagc ggcaagaaag tgcttattta
    19321 tgaccgctca ctctagacaa tatttattaa taatccaatt gttgaactga gagaatatgc
    19381 ataaatcggc aattttttta tgtcgatggg atgagcctga ttcgtttgta aaatcaggca
    19441 aatttttgca aaggcggatg gtcagaattt aaagcaaacc acagcgacgt ttactgcgtt
    19501 cttccgcttc ctgataaagc tggaactcat ccaaaaccgg acaacccaac gcctgctcaa
    19561 atgcctcgcg actggcatta ccgtgattgc gtgcctgcgt ttcattaccc agatttgact
    19621 ggaagatccc cgccgcgcta acgggcaaga aatcttcata ggtaatgggt tgcgccacta
    19681 cccaaccacg ttcaattaag ggctgtggat cgtctccggg atgaatcgcc tgacgatgcg
    19741 cctcacctga aggcgtcaga cggtaccgga accatgccaa cccttgctga cgcattaaaa
    19801 actcactgtc aggaaaagtg cggaaggttt cctgtaaatg catttggtga gtgagattat
    19861 cctgcccggt tccagcgtta cgcagaagat catcatacag ttgtcgccct ttcggcgtta
    19921 atgccacacc acgctgctct atttcaccaa agcgcgcggt atgcgtaccc tgtttctgcc
    19981 ctgcaaacaa caccgtctct tccagtgctt taaagctggt ctggcgtagt aaaataggta
    20041 cctcgcggcg cggcggccct tcgatcagaa ttttgggttc aatcccgcat tcaggcatca
    20101 tcgactgcac ccggtcaata tccagcgtac gtggcgtcag gtggttgata tggcatccag
    20161 gaaaacagac cacatcagca atcaaccgat gttcgttgtg caaggcgcga taggtttctt
    20221 catctaccgt tgctgactgg tgccaacgaa acgtttccag ggcttcctgc acaaactcct
    20281 gtgcctgtgt ttcgttaaaa ccgcgccgct gctcatattc ctctaacagt tgtcgacaac
    20341 gtggggtgaa gatatcgcgc tgacgcagaa tctccgccgc tttctggcgc aaaatttcgt
    20401 tctcgataag ctcaaggcgg agtaatgaag taaaaacgcg gaagggatta cgcgccagag
    20461 aagcatcatc aatgggccga aatgctgtcg aatgtaccgg cacccccgcc tgcgagagat
    20521 cgtaatagct caccgggtac atccccataa tggcaaacat ccgccgaaga gtagcaagct
    20581 cttgtgcagt cccaacacga atcgccccat gacgttcaac attcagtcgc gccagctcgt
    20641 ctgcatttac cattttttcg tgcagttgag gattgttttc cagcacagcc agattcacat
    20701 cagctaccag ttccagcagc gtgccgtact gcggaacttc ttgctggtac atggctgaca
    20761 ttgcctgcga aaactgttcc cgaatctcat ccgccgtgat gctgttcgcc atagtgtctg
    20821 cctccagtgg tcagtaatct ggagtgtagg taaccgcatt cactccggcg ggaagaattt
    20881 acaaactgtg atctcgccgc gaaaacatca atattatcca ttttgctgta acataattgc
    20941 tttaattgtt aataatattt tgcaatcaag ttatcataat caaacaactt cacttgtcag
    21001 cgacaccgct tcgtttttaa catcgcttat ggaaaaaaat agtctgttta gtcagcgcat
    21061 ccgtttgcgc caccttcata cattcgtagc tgtcgcacaa caaggaactt tggggcgcgc
    21121 ggctgaaacc cttaatttga gtcaacctgc gctctctaag acattgaatg aactggagca
    21181 gctgacgggc gctcgcttgt ttgagcgtgg tcgtcagggg gcgcaactta ccttacccgg
    21241 cgaacaattt ttaacgcatg cagtcagagt gcttgacgcc atcaacactg ccggacagtc
    21301 gcttcatcgt aaagaaggtc ttaataatga tgtcgtcagg gttggtgcac taccgactgc
    21361 ggcactgggg atattacctt cggttatagg tcagtttcat cagcaacaaa aagagacgac
    21421 cttgcaagtt gcgacaatga gtaaccctat gattctggcg ggtttaaaaa ccggggaaat
    21481 cgatatcggc attggtcgga tgtcagatcc tgaactgatg accggactta attacgaact
    21541 gctgtttctt gaatcgctga agctggttgt ccgccctaat catccgctac ttcaggagaa
    21601 cgtaacgcta agccgggtgc tggaatggcc ggtcattgta tcaccagaag gcactgcgcc
    21661 acgccagcat tcagatgcat tagtgcagag ccagggatgt aaaattcctt cgggttgtat
    21721 cgaaacgctg tctgcttcgc tatctcgtca acttacggtt gaatacgact acgtgtggtt
    21781 tgtcccttct ggcgctgtaa aagacgacct gcgtcatgcc acgctggtgg ccctgcctgt
    21841 tccgggacat ggtgcaggcg aaccgattgg aatactgacc cgcgtagatg caacgttctc
    21901 ttctggttgc cagttgatga ttaacgctat tcgaaaatca atgccgttct gaaaggtgaa
    21961 gggatctgtc gatcccttcc ttgaacattt tcacaccgta gcgaaactaa ctggttcacc
    22021 cgctccgcga ggttctgccg acacagaatg tttgttcaga cggaatacgt ccaccgcctc
    22081 agttaatcgt gcagcctgtt cttcaaggga caccgccgct gctgaggctt cttctaccag
    22141 agaagcattc tgttgcgtca ccttatccat ttcagaaatc gcctggctaa cctgcgttat
    22201 gcccctactt tgctcatccg aggcggcggc aatttcctgc atgatatgtg tgacactcgc
    22261 gacggcatca acaatagtgc tcatggtttt ccctgccgtt gccacctcat ccgaccccag
    22321 gtcaattaac ctgactgatt cactgatcaa gccttcaatc tctttcgccg cctgagcgct
    22381 gcggcttgcg agtgtccgta cttcgctggc gacaacggca aatccacgtc cttgctcacc
    22441 cgctcgcgcg gcttcaacgg cagcattcaa tgccagaata ttcgtctgga aagcaatact
    22501 gttgatgacg gcggtgatct cggaaatttt cttcgaactc gtggagatag cgcccatcgt
    22561 ttttactaca ccggaaaccg tctgcccgcc atcgctggct ttaatagaag cctcctgcgc
    22621 cagtttgctg gcatgatgcg cgttatctgc attctgtttc accgtcgcag tgagttgctc
    22681 catgctggcg gcagtttgtt caattgccgc cgcttgttct tcagtgcgag atgaaaggtc
    22741 cgcattgcca gctgaaattt cgctggtgcc acgataaatc tcttccgcac cctgtcgaac
    22801 agtccctact gtcatcccca gtgaatgctg catttgctgt aaatgacgac ttaagcgacc
    22861 gatttcatta cgacccgccg gttcatcatt catcgtcaga tcaccactgg cgattttttc
    22921 aatccgttgt gcggcatgtt gcagtggacg aatgacgatc cgacgtagca ctataaatgt
    22981 tatcagcgtc atgaccaggg caagcacaaa cgcgccaatc atgaacatcc cacccaggcg
    23041 ggtgcgctga tgggccagtt ccgccagttg gttggctctg gcgctacgta tcttaacggc
    23101 tttgctcaaa atatcggtat aagcattatc cagcggtcgg atctgctcac tttcatgatt
    23161 gataatcgct tcaaacatgc catttttggc atatttcaac atcggttgca tacccgtgat
    23221 ataagcctgg aagcgttgat ttaattcagt gtcgagggct tcatcagcag gtgttttcac
    23281 cgaccgattc tgataagcac gataaccttg ctgcgactgt ttaatctccg attcggcttg
    23341 cgcaatattt cgcttcattg cttccatttc agcaatacga ctcgcagcac cagcctgaat
    23401 catattgatc cgggctgaac gcaaaaaatc tgaactgttc gctaacccgg tccgaatctc
    23461 aatctccgca gtaacatcac gctgatcgcg atcggcctgc cagaggaaat agccagccag
    23521 agctgaactt aatgcgaata gaactaagat accgccaaga atgcaggcaa ataacggaac
    23581 cagcctgatg tgatgcaaaa aacctaatcg ctgtgaggga gttgtattca ttaccgttgt
    23641 ctctcgtcca ggtttactcc tgatagaaag tcatcggcac ttgacggtaa ttacttatga
    23701 ccaaaaacct ctttgcgtca catttttaca acaattattg cagagaaaat taatgctatt
    23761 ggaatgaaga gagtaacgaa tggctaaacc gaatgacaaa aaattctgga aaacagcacg
    23821 caatttcgtg aagtgattat tttcgtccct aaggaaaact cataggatgc ctgcgggtgc
    23881 ctgagacttt ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat
    23941 ttctatcaac cgaggccaac gaatgaacca agcaattcag atgttagcct cttacccgcc
    24001 atccggcaag gagaaaggct atgaagcgca accctctggt ggtgtgtctg ctcattatct
    24061 gcattacgat tctgacattc acactcctga cccgacaaac gctctacgaa ctgcggttcc
    24121 gggacggtga taaggaggtt gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc
    24181 ggggattttc cccgcgcatc tgactagcag attcattttc tcatggcacc ccttttacac
    24241 ttcagttagt gcatgttttt ttcgatgcac taatagaacc atcattacaa acaaatttgc
    24301 cgcctgccgt acagtgtgag atcccccctt tcgaaccaga acagggtttg cgcccggcat
    24361 cggcataaaa ggaaacaagc gctcccataa ccaagataag tgccaatttt ttcatataac
    24421 ctccatggtt tatcgaatct tatgttcgat ttgaacgtta tacgctggtt tattttgata
    24481 ccgttgatga gatcaaagat cgggtgaatt gattaaaaat aagccagatg gacgtatgga
    24541 taaggattat ggttttattt gtcatacaaa taaatataat aggcgcttcc gatgtagacc
    24601 cgtattcttc gcctgtacca cgggtcggtt ttagtacagg cgttttcttt aaatatcctt
    24661 caggaacgtt tacgcggcat catgcgtaga agtgtgttgt ccttccagaa atagtggtgt
    24721 gccagtgcag ccgcagcgtg caacccgatg acaaaatatc ccagattcgc cagcatttca
    24781 tgccacgact ttaagctatc aacccgttcg aaattggcct ctgaagcgta aggcatcgtc
    24841 aaaccaaacg caaaccatgg gttaccccgg ttatacatca tcaccaaacc aatcactggc
    24901 agcgcaataa acaggagata gatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc
    24961 ggttttggct taggtataat cggcggggtt gggtatttca gccttaacaa aagacgaacc
    25021 accatcagca cgagaattga tatgccacag gaaacatgaa tcatgttgat gagtggtcga
    25081 tcgctacgtg ggaagaaacc acgaaactcc attgcgcaat acgctgcgat aaccagcaaa
    25141 aagaccagcc agtgaatgct gatttgtaac cttgagtatt tattttccat aacatttcct
    25201 gctttaaaac aattttccgt taacataacg agcttttctt aaaatttcat taaatattgt
    25261 tcacccgttt tcagtacgtt acgtttcacg gcaagaatgt agattgccca tcccgccatc
    25321 ctggtctaag cctggaaagg atcaattttc atccgaacgt tcctgacagg agaaaaccat
    25381 gagtaaagtt ggtattaacg gttttggtcg tatcggtcga ctggtattgc gtcgattact
    25441 tgaagtcaaa agcaacatag atgttgtcgc tattaatgat ctcacttccc caaaaattct
    25501 cgcctacctg ctgaaacatg attcaaacta tggaccgttc ccctggcgcg ttgattttac
    25561 ggaagattca cttatcgttg atgggaaaag tatcgcggtt tacgccgaaa aagaggctaa
    25621 aaatattccg tggaaagcga aaggcgcaga aatcattgtc gaatgtactg gcttttatac
    25681 ctccgccgag aaatcgcagg cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc
    25741 ccctgccggt gaaatgaaaa ccatcgttta taacgtcaat gacgacacac tggatggcaa
    25801 cgacaccatt gtttccgtgg cgtcatgcac cactaattgt cttgcaccga tggccaaagc
    25861 cttgcacgac agtttcggaa tagaagtcgg cacgatgacg accattcatg cctataccgg
    25921 cactcagtca ctggtggatg gcccgcgtgg taaagatcta cgcgcttcac gtgcagcggc
    25981 agaaaatatc attccccaca ctacgggggc ggcaaaagcc attggtctgg tgatcccaga
    26041 actgagcggc aaactgaaag gtcatgcaca acgcgtaccc gtgaaaaccg gttcggtgac
    26101 tgaactggtg tctattcttg gaaaaaaagt gactgccgaa gaggtgaaca acgcgcttaa
    26161 aaacgcgaca aacaataacg agtcatttgg ttataccgat gaagaaatag tctcttctga
    26221 tatcataggc agccatttcg ggtcggtgtt tgatgccacg caaaccgaaa ttaccgccgt
    26281 gggcgattta caactggtga aaacggtcgc ctggtacgat aacgaatatg gcttcgtcac
    26341 acagctcatt cgcaccctcg aaaaattcgc taaactctga cgcacacagg cggaggaaaa
    26401 aacctccgcc tctttcactc attacgactg taaataaacc acctgggtct gcaggtattc
    26461 atgcaggcca tgtttgccat ctgcgccgcc aataccggat ttacgccatc cggcgtggaa
    26521 tccttgcata gcttcgaagt tttcacggtt gatgtaagtt tcaccaaact tcagcccttt
    26581 aatggctttc atcgcgacgt tcagattttg ggtatagagt gatgaggtca ggccgtaatc
    26641 actgtcatta gccattgaga tagcctcttc cagcgtgtca aatgcgacca ctggcagcac
    26701 cgggccaaag gtttcctcgt gcataatcga catttcctgg cgaacatcca gcagcaatgt
    26761 cggcggataa t
//
LOCUS       DAYPHN010000058        26572 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000058 DAYPHN010000000
VERSION     DAYPHN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.058"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            179..5599
                     /locus_tag="ACG0BW_002274"
     CDS             179..5599
                     /locus_tag="ACG0BW_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFK7259227.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSACGASLEVDNGQITNINTDIAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRIGSSTGGVGTVNVEGEYSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAIVGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGKVNISNNGLINNKEYSFVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITIKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGAGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMNVGTTGTGTLTLTNNGTLN
                     VEGGEVYLGVFAPAVGTLNIGAAHGEAAADAGFITNATKVEFGLGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVMHDAGHTVFNAGNTYSGKTLVNGGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHAAGTEFA
                     GVAQLKDSTFALERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLTFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGIVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSAQTVETFTG
                     LMGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNKDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTVGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGIYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQVQITWMGVKDSDHIRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDNG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5810..6076
                     /gene="hipB"
                     /locus_tag="ACG0BW_002275"
     CDS             5810..6076
                     /gene="hipB"
                     /locus_tag="ACG0BW_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFK7259228.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6076..7398
                     /gene="hipA"
                     /locus_tag="ACG0BW_002276"
     CDS             6076..7398
                     /gene="hipA"
                     /locus_tag="ACG0BW_002276"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFK7259229.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHCRLSREYGIK"
     gene            complement(7796..8695)
                     /locus_tag="ACG0BW_002277"
     CDS             complement(7796..8695)
                     /locus_tag="ACG0BW_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7259230.1"
                     /translation="MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALKLLEE
                     YIGHPLFVRTTRRIELTETGQLLLEQTAAAVESLENSLESIRDQHQEPSGMVRITLSR
                     FAYLLILKPAMAEFCQQYPGIQLEISVYDGTVNIIKERFDLGIRFGDILEGGVVARPL
                     MKPFREGLYASSAYLSRHGTPEVPADLCHHQLIGYRFITNNRILPLLLNDRGEQLTVE
                     MPGQLISNDIDVMADGIRNGLGIGRLFEPVWQLQPDRERFIPVMESYWKTYPPVYLYY
                     PKNAGKTKRVKALIDFLISTTGR"
     gene            8835..9143
                     /locus_tag="ACG0BW_002278"
     CDS             8835..9143
                     /locus_tag="ACG0BW_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFK7259231.1"
                     /translation="MRILCLDIPAPGASLEKYAPHLNAEALHAWGLYKSGFIRDIYFR
                     QDRPGVAIFLECDSVDEAMNVMAEFPLAKAGLLSFECIPLGSFINWENLFADEFKNKE
                     "
     gene            9152..9841
                     /locus_tag="ACG0BW_002279"
     CDS             9152..9841
                     /locus_tag="ACG0BW_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFK7259232.1"
                     /translation="MKPAYKPVLCVVSSHPIKGASGVPTGFFLAELTHPLKVLEDAGI
                     KTTIASIRGGQPPVDGFDLSDPVNAWYWNETDFQQRLATTPALSELKGADYSAVFFAG
                     GHGTMWDFRDNQDAQRIIREVYESDGIVSAVCHGPAALVDAKLSSGEYLIKGKNVAAF
                     TNKEEEEVHTTDVVPFLLETALREHGALYHEAPNWSENVVTDDRLITGQNPASAHGVG
                     VALLNALRRTA"
     gene            9915..10862
                     /locus_tag="ACG0BW_002280"
     CDS             9915..10862
                     /locus_tag="ACG0BW_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFK7259233.1"
                     /translation="MKTLPPVALGTWSWGTGFAGGDTVFGNHLSDTQMAEVFTAAMSK
                     GLNLWDTAAVYGMGSSETALGALVRQFPRENIILSTKFTPQIADKQSAQPVSDMLEAS
                     LGRLGVDAIDIYWIHNPLDVEKWTPGLIPLLQSGKVKRVGVSNHNLAQIRRANEILNA
                     SGYSLSAVQNHYSLLYRASEEAGILDYCRQNNITFFAYMVLEQGALSGRYDSNHPMPA
                     GSGRAESYNAVLPQIERLTAAMKKMGAERNASVAQIAIAWAIAKGTLPLVGATKVHHV
                     LDAACASDIQLRDEEIILLEQLAAETRVDTRGAWEKPMV"
     gene            10965..11120
                     /locus_tag="ACG0BW_002281"
     CDS             10965..11120
                     /locus_tag="ACG0BW_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259234.1"
                     /translation="MKIICLEENYLDSELGRACMPVALEQAPFLGDWGKTVADGHNPD
                     RLPAFPL"
     gene            11080..11499
                     /locus_tag="ACG0BW_002282"
     CDS             11080..11499
                     /locus_tag="ACG0BW_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFK7259235.1"
                     /translation="MVIILTDSLLSRFNKLNVPLYLHPGLPLKSVQQAYFTGFSAEVN
                     SRLSMFAWGWHHEAGIHLLRLMLSGAFDKYPHLQVISGHWGEMLPFWLQRLDDSLPLA
                     ATGLSRTLTRTFQEHVYVTPSYANTAALPVYLRVNGC"
     gene            11489..11641
                     /locus_tag="ACG0BW_002283"
     CDS             11489..11641
                     /locus_tag="ACG0BW_002283"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFK7259236.1"
                     /translation="MGAERILFSVDYPYQTLDGVKTFIDSLPVNKAEKEAIAFRNAER
                     LLGITV"
     gene            11792..12364
                     /locus_tag="ACG0BW_002284"
     CDS             11792..12364
                     /locus_tag="ACG0BW_002284"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFK7259237.1"
                     /translation="MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPD
                     GKFNIAAEQESLLRADVIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKL
                     ILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
                     KIAQQKTLAREHASRLVELLNSVVNNPEGE"
     gene            12367..13386
                     /locus_tag="ACG0BW_002285"
     CDS             12367..13386
                     /locus_tag="ACG0BW_002285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFK7259238.1"
                     /translation="MQYTRLGKSDLLVSRICMGCMGFGDPSTGQHSWTLDETASRDII
                     RYGLEKGINFYDTAIAYQNGSSERYVGRALREMAKRDDIVLATKFLPRTPAQIAGGIS
                     GKEAIAQSLDQSLKNLGMDYIDLYIYHIWDYNTPIIDVLEALHTAVTAGKVRAVGISN
                     CYAWQLAKANALAEREGLTPFVSVQSHYNLIMREDERELFGLCTEDNIAMTPYSALAS
                     GRLSRKEGYTRRATEDDYARGKYDSTAEQDRSIIERVAELAERHLVSMTEISLAWLLT
                     KVTSPVVGATKKAHVDGAVNAVNLQLSPEEIQFLEETYQPHVLTGIMAQNTPQTKDVK
                     QVWMR"
     gene            13532..14227
                     /locus_tag="ACG0BW_002286"
     CDS             13532..14227
                     /locus_tag="ACG0BW_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFK7259239.1"
                     /translation="MLISSPVFATKPDAPNKPEMNIFELGIRPDRDNDFADVARQTIS
                     ASVDHEAGTLAMYALQRSDNSGQAFMFELYENERAYRKHLNAAPYKAFAARAPDIIDS
                     KKKITLEPQFLGDKHIIPNERTINNLVIVEVKPEFQSEFRNIVLPEMVESLKVEKGVL
                     AMYAGTDSETPKRWYFYEIYASEEDYQLHRQTLHFLDYLRQTAHMSARKDAIPVKPVF
                     LRNKGGIKQEPQR"
     gene            14243..15046
                     /locus_tag="ACG0BW_002287"
     CDS             14243..15046
                     /locus_tag="ACG0BW_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFK7259240.1"
                     /translation="MKSVLAAAAMSLVISDFANAEETRGKAMMKIEPSAISEADIRSV
                     SPALARFGREAITEDLWTRDALSPRDRSVVTVAMLIARNQPAELKHYIDVALDSGVTP
                     AELSEIITHLAFYSGWPNAMSAVSVTKAVFETRGVTPDALPDASPDLLPLNQEAEKQR
                     SETVEKNVGRISPGLVKFTADPLFLDLWQRPALTPRDRSLITVSALIASGQSAQIGYH
                     LNRAMDNGLSAEEAGEIVTQAAFYAGWPNAFTAAPVVGEVLINRSSSKT"
     gene            15449..15715
                     /locus_tag="ACG0BW_002288"
                     /pseudo
     CDS             15449..15715
                     /locus_tag="ACG0BW_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            16149..16712
                     /gene="fimA"
                     /locus_tag="ACG0BW_002289"
     CDS             16149..16712
                     /gene="fimA"
                     /locus_tag="ACG0BW_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFK7259241.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            17075..17785
                     /locus_tag="ACG0BW_002290"
     CDS             17075..17785
                     /locus_tag="ACG0BW_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFK7259242.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            17827..20478
                     /locus_tag="ACG0BW_002291"
     CDS             17827..20478
                     /locus_tag="ACG0BW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFK7259243.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFITSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSIKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGKNKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            20492..21022
                     /gene="ydeS"
                     /locus_tag="ACG0BW_002292"
     CDS             20492..21022
                     /gene="ydeS"
                     /locus_tag="ACG0BW_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259244.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            21035..21538
                     /gene="ydeR"
                     /locus_tag="ACG0BW_002293"
     CDS             21035..21538
                     /gene="ydeR"
                     /locus_tag="ACG0BW_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259245.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            21597..22511
                     /gene="ydeQ"
                     /locus_tag="ACG0BW_002294"
     CDS             21597..22511
                     /gene="ydeQ"
                     /locus_tag="ACG0BW_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259246.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            22844..25123
                     /gene="ydeP"
                     /locus_tag="ACG0BW_002295"
     CDS             22844..25123
                     /gene="ydeP"
                     /locus_tag="ACG0BW_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFK7259247.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLNWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            25371..25568
                     /gene="safA"
                     /locus_tag="ACG0BW_002296"
     CDS             25371..25568
                     /gene="safA"
                     /locus_tag="ACG0BW_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFK7259248.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            25643..26404
                     /gene="ydeO"
                     /locus_tag="ACG0BW_002297"
     CDS             25643..26404
                     /gene="ydeO"
                     /locus_tag="ACG0BW_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFK7259249.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRSQRHTGMNKGNTMNALAI"
BASE COUNT         7283 a         5980 c         6496 g         6813 t
ORIGIN      
        1 aagaactatg caatttttaa tctatcgctt cgaaagtatt agcaaattag ccaaattgac
       61 accttcccac ctccaaccaa cactcaatac agacctctga caatttaata agtttcattt
      121 ttaatgtctg acgactcatc tctttcgtgg ctgtattgtt tgtatatgga gcgtttatat
      181 gaatagaatc tatcgcgtga tatggaattg caccctacag gtatttcagg cctgctcgga
      241 attaactcgc agggtaggta aaacatcaac ggttaatttg cgtaaatcct ctggactgac
      301 aacgaaattc agtagattga cgctgggtgt tttgctggca ctaagcggtt cagcgtgtgg
      361 tgcgagtctg gaagttgata atggtcagat taccaatatt aatactgata ttgcttacga
      421 tgcctacctg gttgggtggt atggcactgg agtgcttaat attttggctg gcggtaatgc
      481 ttccttaacc actattacta ccagcgtcat tggcgctaat gaggactcgg agggtaccgt
      541 taatgttttg ggtggcacct ggcgattgta tgatagcgga aataatgcaa ggcctttaaa
      601 tgtgggtcaa tccggtacgg ggacgctgaa tattaaacag aagggtcacg ttgacggagg
      661 atatttaagg ataggttctt cgacaggagg cgtcgggacg gtcaatgttg agggagagta
      721 ctcggttctg acgaccgaat tattcgaaat agggagttac ggtacaggct cattaaatat
      781 tacggataag ggttacgtca cgagttcaat cgtcgccatt gtaggatatc aggcgaacag
      841 caatggtaag gttgtcgttg aaaagggtgg cgagtggcta ataaaaaata atgattcctc
      901 aattgaattt caaattggca atcaaggcac gggggaagcg actattcgcg agggggggct
      961 gattacggct gaaaatacga ttatcggtgg caatgccacc ggtatcggaa ccctgaatgt
     1021 gcaggatcaa gactctgtta tcacggtacg cagactctat aatggatatt tcggtaatgg
     1081 caaagtcaat atttccaata atggactgat taataacaaa gaatattcat ttgtgggcgt
     1141 tcaggacggg tcccacggtg tcgtcaacgt gaccgataaa gggcattgga atttcctcgg
     1201 aacgggcgaa gctttccgct atatctatat cggtgatgct ggcgacggtg aacttaatgt
     1261 ctcgagtgaa ggaaaagtag attcgggaat tatcactgcg gggatgaaag aaacaggcac
     1321 aggcaacatt actattaagg ataagaactc cgttatcact aatctcggaa ctaatcttgg
     1381 ttatgacggc cacggcgaaa tgaatatcag taatgagggg cttgttgtca gcaacggagg
     1441 aagctcactc ggttatggag aaaccggcgt cgggaatgtc agcatcacca cgggggggat
     1501 gtgggaggtc aataagaatg tctataccac cattggtgtt gcgggtgtcg gaaacctcaa
     1561 tatcagcgat ggcggtaagt tcgtatcgca aaatattact tttttgggcg ataaagcaag
     1621 cggtatcggc acactgaacc tgatggatgc gacatcatcg ttcgatactg taggtatcaa
     1681 tgtcggtaat tttggtagcg gcatcgtaaa tgtcagtaat ggtgccactc ttaattcaac
     1741 gggctatgga tttatcggag gaaatgcctc cggtaagggg atagttaata tttcaacgga
     1801 tagtctctgg aatttaaaga catcatctac taacgcccaa ttactacagg tcggtgtatt
     1861 aggcgcgggt gaactgaata ttaccaccgg aggtatagtt aaagcgcgtg atacacaaat
     1921 agctctcaat gacaaaagta agggcgacgt gcgggtggat gggcagaact ctcttcttga
     1981 aacattcaat atgaacgtag ggacgactgg cacaggtacg ttaaccctga cgaataacgg
     2041 tacgctgaat gtcgaaggtg gagaagttta cttaggtgtt tttgcgcctg ctgtaggaac
     2101 gctaaacatt ggtgctgctc acggtgaggc ggcggcagat gccggattta tcaccaatgc
     2161 gacgaaagtg gagtttggtc ttggcgaagg cgtttttgtc tttaatcata ccaataacag
     2221 tgatgccggc taccaggtcg atatgctgat tacgggtgac gataaagacg gaaaagtgat
     2281 gcatgatgca ggccatacgg tgttcaatgc agggaatact tatagcggta aaacgttggt
     2341 caatggcggc ctcctgacca ttgcgtcaca tacggcagat ggggtaactg gcatgggttc
     2401 gagtgaagta accattgcaa gccccggtac gctcgacatt ctcgcatcaa cgaacagtgc
     2461 gggagattac acgctgacca atgcgctcaa aggcgatggc ttgatgcgag tgcagctgtc
     2521 atcctccgac aaaatgtttg gctttaccca tgcagcaggg actgaattcg ccggtgttgc
     2581 ccaactgaaa gacagtacct tcgcactgga acgcgacaac accgctgcgc ttactcacgc
     2641 gatgctgcag tctgacagtg aaaataccac atcggtaaaa gtgggagagc aatccattgg
     2701 tggactagcc atgaatggcg gtacgctcac tttcgatacg gatatacctg ctgcgacgct
     2761 tgcggaggga tatatcagcg tcgatacact ggttgtcggc gcgggtgact acacctggaa
     2821 aggccgtaac tatcaggtaa acgggacggg cgacgtgctt atcgacgtgc ctaaaccgtg
     2881 gaatgatccc atggcgaata accctctgac gacgctcaat ttgctggaac acgatgatag
     2941 ccatgtcggc gttcaactgg tgaaggcgca aacggttatt gggtcgggtg gctcattaac
     3001 gttacgtgat ttacagggcg acgaggtgga agcggacaaa acgttacaca ttgcacaaaa
     3061 cggaatagtg gtcgccgagg gtgattatgg attccgcctc acgaccgcac caggtgatgg
     3121 tttgtacgtt aactatgggc tgaaagcgct gaacatccat ggtgggcaaa agctgacgtt
     3181 agccgaacat ggcggagcct atggcgcaac ggccgatatg tcggcaaaaa tcggtggtga
     3241 aggggatctg gcaatcaata cggtgcgaca ggtttcgctt tctaacggtc agaacgacta
     3301 tcagggggca acctacgttc agatggggac attacgtacc gatgcggatg gcgcgctggg
     3361 caatactcgg gaactgaaca tcagcaacgc ggccatcgtc gatcttaatg gatcggccca
     3421 gacggtagag acattcaccg ggctgatggg ttcgactgtt ttgtttaaag aggggtcgct
     3481 gacggtgaat aaaggtggga tcagtcaggg cgaactgaca ggtggcggaa acctgaatgt
     3541 tacaggggga acactggcta tcgaggggct taatgcacgc tacaatgcgt taaccagcat
     3601 tagcccaaat gccgaagtca gcctcgataa tacgcagggg ttaggcagag gaaatattgc
     3661 caatgacggt ctgttaacgc taaaaaatgt gaccggcgaa ctgcgtaata gcataagcgg
     3721 gaagggtatc gtgagcgcaa ccgccaggac agatgtagag ctggatggcg ataatagccg
     3781 ctttgtgggg caattcaaca ttgatacagg cagcgcgctc agcgtcaacg agcagaaaaa
     3841 cctgggtgat gcttccgtta tcaataatgg cctgctcacc atctccactg agcgtagctg
     3901 ggcgatgacg cacagtatca gcggtagcgg tgatgtgaca aaactgggga ccgggatcct
     3961 gactcttaac aaagattccg cggcgtatca gggtacgacg gatatcgtgg gtggtgaaat
     4021 tgctttcggt tccgactctg ccattaatat ggcaagtcag cacattaata tccataacag
     4081 cggtgtgatg tcgggaaatg tcaccactgc aggtgatgtg aacgttatgc caggggggac
     4141 actgcgtgtc gctaaaacca ctgtcggcgg taacctggag aatggtggca cggttcaaat
     4201 gaacagcgaa ggaggaaaac cggggaatgt actgaccgtt aacggtaact ataccggaaa
     4261 caatggcctg atgacgttca acgcgacgct gggcggcgat aattcgccca ccgataagat
     4321 gaacgtgaaa ggcgataccc aagggaatac tcgcgttcgg gttgataaca ttggcggcgt
     4381 cggtgcacaa actgtcaacg gtattgaact cattgaggtc ggcggtaatt ctgcaggtaa
     4441 cttcgcgctg accaccggaa ctgtcgaagc tggggcttac gtctacacgc tggctaaagg
     4501 gaaggggaat gacgagaaaa actggtatct gaccagtaaa tgggacggcg taacgccagc
     4561 ggatacaccc gatcccatca ataatcctcc tgttgtggat ccggaaggcc catcagttta
     4621 tcgcccggag gccggaagct atatcagcaa cattgccgca gccaactcgc tgtttagcca
     4681 tcgtttacac gaccgtctgg gtgagccgca gtatacagat tcactgcatt ctcagggttc
     4741 ggcaagcagt atgtggatgc gtcatgtcgg agggcacgaa cgttcaaggg ccggtgacgg
     4801 tcagctaaat actcaggcta accgctatgt attgcagcta ggcggcgatt tggcgcagtg
     4861 gagtagcaac gcgcaggatc gctggcatct cggcgtcatg gcaggctatg ccaatcagca
     4921 cagtaatact cagagtaatc gtgtgggtta taaatcggat gggcgcatca gcgggtacag
     4981 cgctgggatt tacgcgacct ggtatcagaa cgatgcgaat aagaccggcg cttatgttga
     5041 cagctgggcg ctgtataact ggtttgataa cagcgtcagt tccgataacc gttctgctga
     5101 cgactatgat tctcgcggtg tgacggcctc tgttgagggt gggtatacct ttgaagcggg
     5161 aacatttagc ggcagcgaag ggacgctgaa tacctggtac gtccagccac aggtgcaaat
     5221 cacctggatg ggtgtgaaag attctgacca tatccggaag gacggaacac gcattgaaac
     5281 ggaaggtgac ggaaacgtgc aaacgcgact tggggtgaaa acctacctga acagtcatca
     5341 ccagcgtgac aatggtaaac agcgtgagtt ccagccttac attgaagcga actggatcaa
     5401 caacagcaaa gtctacgccg tgaagatgaa tggtcaaacc gtaagccgtg atggtgcgcg
     5461 taatctcggt gaagtacgta ccggggttga ggcgaaagta aataacaacc ttagcctgtg
     5521 ggggaatgtc ggtgtgcaac taggtgataa aggctatagc gatactcagg gcatgctggg
     5581 tgtgaaatat agctggtaaa ccgtataagc cgcatgtcga agatgcatgc ggcttaatat
     5641 tgccgacttc aaacgacttt taaatcctcc tttttatccg cgatcgcgga tatcgcagcg
     5701 tttatcccgt agagcggata agatgtgttt ccagattgac tttatcctca ctaaaggata
     5761 aaactgataa tatccccttg agcggataaa tatgctgtgg acgtatgaca tgatgagctt
     5821 tcagaagatc tatagcccaa cgcaattagc gaatgcaatg aaactggttc gtcagcaaaa
     5881 tggctggacg cagagcgagc tggcgaaaaa aatcggtatt aagcaggcga caatttccaa
     5941 tttcgaaaac aatcctgaca ataccacgct gacgacattt tttaagattt tacagtcgct
     6001 tgaactctca atgacgctat gcgacacgaa aaatgcctcg ccagaatcaa cagaacagca
     6061 agatctggag tggtaatgcc taaacttgtc acttggatga acaaccagcg ggtaggcgag
     6121 ttaacgaagt tagccaatgg cgcgcacacc tttaagtatg caccggagtg gttagccaac
     6181 cgttatgcca gaccgttatc actttcgctg ccattgcaga gggggaatat cacctctgat
     6241 gccgtattta acttcttcga taacctgtta cccgatagcc cgattgtacg tgaccggatc
     6301 gttaaacgtt atcatgccaa atccagacaa ccgtttgatt tattgtcaga aatagggcga
     6361 gacagcgttg gtgccgtgac gttaataccc gaagacgaaa ccgtaacgca tccgataatg
     6421 gcatgggaaa agcttactga agccagactt gaagaagtat taacggctta taaagcagat
     6481 atccctctgg gcatgattag agaagaaaat gactttcgca tctcggttgc tggcgcgcag
     6541 gagaagacag cgctgctcag aataggcaat gactggtgca ttccgaaagg aataacgccg
     6601 acgacgcaca tcattaaatt accgattggc gaaatcaggc agcccaatgc gacgctcgat
     6661 ctcagccaaa gcgttgataa tgagtattac tgtctgctgc tggcgaaaga acttgggttg
     6721 aatgtgccgg acgcagaaat cattaaagcg ggaaatgtgc gcgcgttagc ggtcgaacgt
     6781 tttgacaggc gttggaatgc tgagcgaacg gttttacttc gcttgccaca ggaggatatg
     6841 tgtcagacat tcggtttacc ttcatcggtg aaatatgaat cagatggagg cccaggcatc
     6901 gcgcggatca tggctttttt gatggggtcc agcgaggcgc tgaaagatcg ctatgatttt
     6961 atgaagtttc aggtgttcca gtggttgatt ggcgcaacgg atggccatgc aaaaaacttc
     7021 tccgtattta ttcaggctgg cggcagttat cgactcacgc cattttacga catcatttca
     7081 gcatttccgg tattgggtgg cgcaggaata cacatcagcg atctcaaact ggcaatgggg
     7141 cttaacgcat ccaaaggcaa aaaaacggca atcgataaaa tttatccgcg acattttctg
     7201 gcgacagcaa aggtgctgaa atttccggaa gtgcagatgc atgaaatcct gagtgacttt
     7261 gccagaatga ttccggcagc actggataac gtgaagactt cattaccgac agattttccg
     7321 gagaacgtgg tgacggcagt tgaaaccaat gtgttgaggt tgcactgtcg cttaagccga
     7381 gaatacggta ttaagtaaga tatggggttt ggccgttgtt aatgagcagg acaatcatga
     7441 ccgccaacga ctggattctc ccttggttta tgttggtgcg cataatgtat attatgttaa
     7501 atatggtggg tttgttctta agttcatgtg ttccgctctt tcagtagctt aactgatgaa
     7561 tcagtcagca gcttttgccg tgcaagctac gcggcggttc gtaccagatt ggccacctcc
     7621 atctcctggt tcgctttccc ctccccccgg taaacctcac ccaacgttaa ttaatgatgt
     7681 cagtatgtgg gcgctatcct tgtatgtaaa atccactgac gattacgccc atctgtgaaa
     7741 aaaaactcac aatgccattc gatgcatctc cataccgaac gaggcgttat cgtctttatc
     7801 gccccgtagt ggatatcaga aaatcaatca gggccttcac tcttttcgtt ttacccgcgt
     7861 ttttggggta atagagatac actggcgggt aggttttcca gtagctctcc atcaccggaa
     7921 taaagcgctc cctgtccggc tgtaactgcc agacaggctc aaacaagcgc ccaattccca
     7981 gcccgttacg gatcccgtct gccataacgt caatatcgtt gctgattaac tgccccggca
     8041 tctcaacggt caactgctca ccgcgatcgt tcaggagcag cggaaggatg cggttgttgg
     8101 tgataaaacg gtaaccaatc agctggtgat gacagagatc tgccggcact tcaggcgttc
     8161 catgtcggga gagatacgct gaggatgcat ataatccttc acgaaacggt ttcattaacg
     8221 gtcgggccac cacccctccc tcaagaatat cgccaaaacg gatccccaga tcaaaacgct
     8281 ctttgatgat attcacggta ccgtcgtaga ccgaaatttc taactgtata cccggatatt
     8341 gctgacagaa ttctgccatc gcaggcttaa gaattaacag gtaggcaaaa cgcgagagcg
     8401 taattcgcac catccctgag ggctcctgat gctggtcgcg aatgctttca agtgagtttt
     8461 ccagcgactc caccgccgca gcggtctgtt ccagcaataa ttgaccggtt tccgtcagct
     8521 ctatacggcg ggtcgtacgc acaaaaagcg gatgaccgat atactcttcc agcagcttta
     8581 gtgcattgct gacggaggga ggcgtaattt ccagtttcct tgcggctgcc gaaatattgc
     8641 cctcccgcgc aatgctctgg aagatgcgaa tctggttata aagagcgtta ttcataatgt
     8701 tttcctaaag caattctcgc ctgattatta agtacagtct aaaaatgctt aagtcaatga
     8761 ccctctaatc ttcctgaaat acgcctctta tactttagtc tctttctact acgacctgag
     8821 ttcaggagac tatgatgcga attttatgtc tggatatccc tgcccctgga gcatcgctgg
     8881 agaaatatgc tccgcacctc aacgctgaag cgctgcacgc ctggggattg tataaatcag
     8941 gcttcatccg cgacatctac ttccgtcagg acagacctgg cgtcgctatt tttcttgaat
     9001 gtgactctgt cgatgaagcg atgaacgtta tggccgaatt cccgttggca aaagcaggct
     9061 tactaagctt cgagtgtata ccgcttggct cctttattaa ctgggaaaat ctcttcgccg
     9121 atgaatttaa aaataaagag tgaggaccga tatgaaacct gcatataaac cagtgctatg
     9181 tgtcgtttcc agccatccga ttaaaggcgc atccggtgta ccaaccggtt ttttcctggc
     9241 cgagctgact catccgctga aagtactgga agatgccgga ataaaaacaa caatagccag
     9301 tattcgcggg gggcagccac cggtggatgg atttgacctc agcgaccctg ttaacgcctg
     9361 gtactggaac gaaaccgatt ttcagcagcg cctggcaaca acacctgcgt tatccgagct
     9421 gaaaggtgcc gattacagcg cagttttctt tgcaggtggt catgggacca tgtgggattt
     9481 ccgtgataat caggatgccc agcgtattat ccgcgaagtg tatgaaagtg atgggattgt
     9541 ctcagccgtc tgccacgggc ctgcggcatt ggttgacgca aaactcagta gcggcgaata
     9601 cctgataaaa gggaaaaatg tggcggcctt taccaacaag gaagaggaag aagttcacac
     9661 aacagatgtg gttcccttcc tgcttgaaac cgcacttcgc gagcatggtg cgctttatca
     9721 tgaagctcca aactggtctg aaaacgtggt taccgacgat cgccttatca cggggcagaa
     9781 tcccgcttca gcacatgggg ttggtgtggc gctgttaaat gcactccgcc ggacagctta
     9841 attaattcat cgttactcac cttacttggc cggtttaagt cagccacgat ccggcctttt
     9901 ttcaggagct gaatatgaaa acactacctc cagtcgcact aggtacatgg tcctggggca
     9961 caggctttgc cggcggcgac accgtctttg gtaatcatct ttctgatact cagatggcag
    10021 aagtgttcac tgcggccatg agtaagggcc tcaatctctg ggataccgca gcagtatatg
    10081 gtatggggag ttccgagact gcgctgggag cattagtccg tcagtttccc cgcgagaaca
    10141 taattttatc caccaaattc acgccgcaga ttgcggacaa gcagtctgcg cagcctgtca
    10201 gcgatatgct tgaggccagc cttgggcgcc tgggcgtgga cgcgattgat atctactgga
    10261 tccataatcc cctcgacgtc gaaaaatgga caccaggact gatcccactt ttacaaagcg
    10321 gtaaggtaaa acgcgtaggt gtttccaacc acaatctggc acaaatcaga cgcgccaatg
    10381 aaatccttaa cgcttccggt tattccctca gtgcagtaca aaatcactac agcctgctct
    10441 atcgcgcttc tgaagaggcc gggatccttg attattgccg acaaaataac atcacgttct
    10501 ttgcttacat ggtcctggag caaggcgcgc tcagtggtcg ttatgattca aatcatccca
    10561 tgcctgccgg gagtggccgg gccgaaagtt ataacgcagt actgccgcag atagaaagac
    10621 taaccgccgc catgaaaaaa atgggagccg aaaggaatgc cagcgtggca cagatagcca
    10681 tcgcatgggc tattgcgaaa ggtacccttc ccctcgttgg tgcgactaaa gtccatcacg
    10741 ttctggacgc cgcctgcgct tcagacatcc agttacgcga tgaggaaatc atcctgctgg
    10801 aacaactcgc cgcagagacc agagtggata cacgaggcgc ctgggaaaaa ccgatggtgt
    10861 aaagcccgtc cggaacagac tgtcatgaat gacacacacg aacgggctgt tccgggtgat
    10921 tctaatctgt atcactttta ctgctttgta tgaatggtga gaatatgaaa atcatttgcc
    10981 tcgaagaaaa ttatctcgac agcgagttag ggcgagcgtg tatgcctgtt gcgcttgagc
    11041 aggcaccttt ccttggcgac tgggggaaaa ccgtcgctga tggtcataat cctgaccgac
    11101 tccctgcttt cccgctttaa taaactgaat gtgccactct atcttcatcc cgggttaccg
    11161 cttaaaagcg tacagcaggc ctacttcacg ggcttcagcg cagaggtcaa ctccaggctt
    11221 tctatgtttg cctggggctg gcatcacgaa gcggggatcc atttgttgcg gctgatgtta
    11281 tcgggtgcat ttgataaata tccccacttg caggtaatca gtggccactg gggtgagatg
    11341 ttacccttct ggctccagcg tcttgatgac agcctgccac tggctgcaac gggtctgtca
    11401 cgcacgctaa cgagaacctt ccaggaacac gtttacgtta ccccgtcata tgctaacact
    11461 gccgcacttc cagtttatct acgcgttaat gggtgctgaa aggatcctgt tctccgtcga
    11521 ttatccctat cagaccctgg acggtgtaaa aacctttata gacagtctgc ccgtcaacaa
    11581 ggctgaaaaa gaggccatcg cgtttcgcaa tgcagaacgt ttactgggca tcacggttta
    11641 gttcaccgtt tcatgatgac tgagcacatt gtgatcagcc gacaattatt agtttttgcc
    11701 taaaagtgaa tgagccttta ctgctctaaa aaaataatgt tgtcactcta tactggattt
    11761 aatattcacc aaagatgttt aactgattga aatgaaaaat atccttgtta tttcaggtca
    11821 tcctgagctt acccattccg tcgccaatgc cactattctt gatgaagtgg agaccgccct
    11881 tcctgatgtt gagattcgtc gtctggacag gctctatccg gatggcaaat tcaacatcgc
    11941 ggcggagcag gaaagcctgc tcagggccga tgtgattgtc tggcagttcc ctttttcctg
    12001 gtatgggctg ccaggattaa tgaaaaaatg gctggacgag gtctttgttc acggcttcgc
    12061 gcatggctca acggcgaaac tgggcggtaa aaagctgatc ctctccttta caacgggagc
    12121 gccagaggag ctctataccg tcgacagttt ctttggccat accattgaag agtatcttat
    12181 tccgttcgaa accacggcaa ggctgtgcaa tcttgagctg ctggcgccgg tttatacctg
    12241 cggtatcagc tatgccgacc gggatgccga caaaatcgcg cagcaaaaaa cgcttgcccg
    12301 ggaacacgct tcaaggcttg tcgaactgct caattccgtc gtgaataatc cagagggaga
    12361 ataacaatgc agtatacccg gctcggtaaa agtgacttac ttgtttcccg catctgcatg
    12421 ggatgcatgg ggtttggcga tccgtcgacg ggtcagcata gctggacgct ggacgaaaca
    12481 gcaagccgcg acatcattcg ctatggcctt gaaaagggca tcaactttta cgataccgct
    12541 atagcctacc agaatggctc cagtgagcga tacgttggcc gggcattacg ggagatggcg
    12601 aaacgcgatg atatcgtcct ggccaccaag tttctgccgc gaacccccgc acaaattgcc
    12661 ggagggatca gcggaaaaga ggcaatcgct caatcccttg accagagcct gaagaatctg
    12721 gggatggact atatcgacct ctacatctac cacatctggg attacaacac gcccattatc
    12781 gacgtgcttg aagcgttaca taccgctgtt actgctggca aagtacgagc tgttggtatt
    12841 tcaaactgct atgcctggca gctggcgaag gcgaacgccc ttgctgaacg cgaagggctg
    12901 acgccctttg tttccgtaca gagccactac aatctaatta tgcgcgaaga tgaacgggag
    12961 ctcttcggcc tgtgcactga ggataatatc gcaatgaccc cctatagcgc gctggccagc
    13021 ggccgcctct ctcgtaaaga aggctataca cggcgagcca cagaggatga ctatgcgcgt
    13081 ggaaaatatg acagcacggc tgaacaggat cgcagcatta ttgagcgtgt agccgaactg
    13141 gccgaacgcc atctggtgtc aatgacggaa atatctctcg cttggctgct gacaaaagtc
    13201 acatcacctg tcgtaggggc tacaaaaaaa gctcatgtcg atggcgcggt aaacgcagta
    13261 aatcttcaac taagtccgga ggaaattcag tttctggaag aaacctacca gccgcatgtt
    13321 ctgacgggga tcatggcgca aaatacgccg caaacgaaag atgttaagca ggtctggatg
    13381 cgatagccct tattcacttt tcctcctctc gtctttgaga gcgccagtag cggtcggtta
    13441 cgttcttacc agcaccagtt gatcgaacgt cttaaacagt aatgtcagta aaaagatcat
    13501 cgtgaaaaag acttttatgc ttttaatttg catgctaata agcagccctg tctttgcaac
    13561 aaaaccggac gcgccgaata agccggagat gaatatcttc gagctcggca tcagacccga
    13621 tcgggacaac gactttgcag acgtggccag acagacgatt tccgcttcgg ttgatcatga
    13681 agcaggtaca ctggcgatgt atgccctgca acgcagcgac aattcaggtc aggcattcat
    13741 gttcgaactc tatgaaaatg agcgtgctta tcgcaaacat ctcaatgccg ccccatacaa
    13801 agcattcgct gctcgtgcac ctgacattat tgacagtaag aagaaaatta ccctggagcc
    13861 acagtttctg ggcgacaaac acatcatacc gaatgaacga actatcaata atctggtgat
    13921 cgtcgaagta aagcctgaat ttcagagcga atttagaaac atcgtccttc ctgaaatggt
    13981 cgagtcgctc aaagtagaga aaggcgtgtt agccatgtat gccggtacag attcagagac
    14041 tccaaaacgc tggtatttct atgagattta cgccagtgag gaggattatc aactgcaccg
    14101 acaaacgctg cacttcctgg attacctcag gcaaacggcg catatgagcg ccaggaagga
    14161 cgcaatcccg gtaaaaccgg tatttcttcg taacaaaggc ggaatcaaac aggagccgca
    14221 acgttgacca gtaaggatta acatgaaaag cgtacttgca gcagcggcta tgtcgcttgt
    14281 tatttctgat tttgccaatg ctgaggaaac aagaggtaaa gctatgatga aaatagaacc
    14341 gtcagcgatt tcggaagctg atattcgttc cgtctcaccg gctctcgcgc gctttggtcg
    14401 cgaagctatt actgaagatc tgtggacacg cgacgccctc tcaccgaggg atcgtagtgt
    14461 ggtgaccgtc gccatgctga ttgcccgcaa ccagcctgcc gagcttaagc actatattga
    14521 cgtcgctctc gatagcggcg tgacgccagc ggaattatca gaaatcatta cccatctggc
    14581 gttctactca ggttggccaa atgcaatgtc agccgtcagc gtgaccaaag cagtatttga
    14641 aactcgtggc gtcacgccag atgcgcttcc tgacgcttct ccggacctgc ttcctctcaa
    14701 tcaggaagca gaaaagcagc gttcagagac agtggaaaaa aatgttggcc gaatatctcc
    14761 aggcttggtt aaatttactg ctgacccact gttcctggat ctctggcaaa gacccgcgct
    14821 aaccccccgt gatagaagcc tgattacggt cagcgcgtta atcgcatctg gacagagcgc
    14881 gcagattggc tatcacctta acagagcgat ggataatgga ctttctgctg aggaagctgg
    14941 ggagatcgtt acccaagccg cgttttacgc tggctggcca aacgcattta ccgctgctcc
    15001 tgttgtcgga gaggtattaa tcaatcgctc atcgagtaaa acgtaatatt actgaacacc
    15061 aaaacctgac gcacatcaca acattagaac gaacagttca tgaaccaaca gacgtgacat
    15121 cgagtgattt atggacagcg tttaacacag ttatgaacca aaactgagtt cataactgtg
    15181 agtttgaatc gtcatttgat taatatccat ttaaaacagt ttgttatgct gtttctggtg
    15241 ctgaagtcaa ttatgtatat ccctcaaacc cagaatagtt aaatggaatt tgagtgatgc
    15301 gaagcgattt tacggtcgtc gttattactt cagggttcca gaacataaag cctctcctct
    15361 ttcatgattg gcctgtgcat accggatata ggctggctgg tatgcaggtt tgccgtttgc
    15421 cgcatcttcc ctgcgttgaa gagtcaggct ggcaaaccgc gtgcaaatga acacaccctc
    15481 tttccatcaa cacttcagac agcttgcggg aatgagtcca ctccggtatc agaaatggct
    15541 acgcctcaat gaagccaggc gactgatgct gaatgaacat tacgatgtca ccactgcagc
    15601 ctatgctgtc ggttacgaaa gcttatccca tttcagtcgg gaatattcgc ggatgtttgg
    15661 agagtcacct aagagagata ttgccaggtt aagaaagtct gtcgatcagc tttaataaac
    15721 cctgcattta tctggttaga gcaatttggc gttagtttca gaaagcaaac attaaccata
    15781 gctaatgatt tttagccata taaaccatta gtaactcttg acctttcctg acacttccct
    15841 tgttcgcaat aatattctgt ttagtcgctg tttataaata acatgcataa tgaaatcgta
    15901 atgttattta gcgacataaa atgtctttgc aaatactctc tacaacaagt gctgcaataa
    15961 acttgttgtg attaggtgtc cttgtaattt aaagatatta tcgttcttcg tgaaatgtaa
    16021 taaccctgga aactatattc tcagggcaat atcccatcaa gaatgattct tgttttataa
    16081 gcaataacag aatgacttga ttgttaactt tctttattaa gctacttaaa tataaatttt
    16141 ggagaattat gaaactcaaa catgtcggta tgattgtcgt ttctgtgttg gcgatgtcgt
    16201 ctgctgcggt aagcgcagcc gagggtgatg aatcagtaac gaccactgtt aatggcggtg
    16261 ttattcattt taaaggtgaa gtggtaaatg ccgcttgtgc gattgattca gaatcaatga
    16321 accaaacggt tgagctgggt caggtccgtt cttctcgtct ggctaaagcg ggtgacctca
    16381 gctccgccgt tggcttcaat atcaagctga atgattgtga taccaatgtt tccagtaatg
    16441 cagctgttgc attcctgggt actactgtca ccagtaatga cgatacgtta gcgctgcaga
    16501 gttcagcggc aggctctgcc caaaatgtcg gtattcaaat tttggaccgt acgggtgagg
    16561 tattaatact tgatggtgcc acttttagtg ctaaaactga cttgattgat ggcacgaata
    16621 tattgccgtt ccaggctcga tatattgctc tcgggcagtc agtagctggt actgcaaacg
    16681 ctgatgcgac cttcaaagtt caatatctat aattcaatct taaggtggct atacgtcatc
    16741 tgaaattgaa acagggtgtg tgtggcatgg ctacgcacac ccgagcaatt caccaatgtt
    16801 catctcatta ttaatgagga atagtttgtg ttttggagcg gtatgaaaaa atcacataat
    16861 atctaaacga tcaataaaca acagtagtta aacttactgt tatttaaaat atttactttg
    16921 tagcaaggtt ttcctattaa aagagaacca acatttatta caaacgcatt aagtctggaa
    16981 acccggcgag tcgccaatgt attagcaata tttactgatt gtctgatgta acggttttcc
    17041 cgcttatcct cttaacacat ggagaatgca tcagatgcaa actacgcgta ccccctactc
    17101 tatttccttt atggcgacag tgttactttt attgctcttt gcttgccata gcaccattgc
    17161 caacgcagct gtcgcactgg gtgcgacgcg ggtaatttat ccggcaaacc agaaacaggt
    17221 tttattgcct gtcacgaata atgatcctgc cagtgtctat ctcattcagt catggataga
    17281 aaatgcaggt gaccaaaaag acacccagtt tgtcattacg ccaccgcttt tttccatgca
    17341 aggtaaaaaa gagaatacgc tgcggataat caacgccact aatcatcagt taccaggcga
    17401 ccgtgaaagc ctgttctggg ttaatgttaa agctattcct gctatggaaa aagatcagaa
    17461 gaatgagaat acattacagc tggcaattat tagccgcata aaaatgtttt atcgccccac
    17521 gaacctggca atggcacctg aagaagcgcc tgcaatgctt cggtttcgtc gcagcggcag
    17581 caaactcact cttattaatc caacgcccta cttcattacc gtaacaaata tgaaagcagg
    17641 aaatagcaat ctcccaaaca ccatggtgcc gccaaaaggt gaagtatcgg tggatatccc
    17701 gcatgctgca accggggata tcagttttca gacgatcaat gattacggtg cattaactcc
    17761 gcgtattaag gcaaccatgc agtaagccag tgacctaatt aataataaac tcaatcggaa
    17821 ttaataatga cagcctttcg tgccgcattt aaggcatatc gtatgcatca ggtgttacta
    17881 ctgcctcgct ttgcacggct taccattgcc cttggcttag caactgcggt atttcccgtt
    17941 gacgcagagt attattttaa tccacggttt ttaagcaatg atcttgctga atctgtcgat
    18001 ttatccgcct ttactaaggg acgcgaggca ccacctggta catatcgggt cgacatttat
    18061 ttgaacgacg aatttattac tagtcgggat attactttta ttgcagatga taataacgca
    18121 gatctcatcc catgcctgag tacagatctt ctagtcagtc ttggcataaa aaaatcagcg
    18181 ttattagaca ataaagaaca ttcggcagaa aaacatgtgc cggacaacag cgcatgcaca
    18241 ccacttcagg atcgtttggc cgacgcatca actgagtttg atgttggtca gcaacatctg
    18301 tcactctccg ttccgcaaat ttatgttggc agaatggctc gcggctatgt ttctcctgat
    18361 ctgtgggaag aaggaataaa tgctgggctt ctaaactata gttttaatgg taattctatt
    18421 aataatcgta gtaaccataa tgcaggaaaa tccaactatg catatttgaa tttacagagt
    18481 ggcatcaaca ttggtagttg gcgactgcgc gataactcaa cgtggagtta taacagtggg
    18541 agtagcaatt catctgacag caataaatgg cagcatatca atacgtcggc tgaacgtgac
    18601 attattccct tacgctcacg cttaacggta ggtgatagtt ataccgatgg cgatattttt
    18661 gatagtgtga actttcgtgg cctcaaaata aattcaacag aagcgatgtt gcccgatagc
    18721 caacatggtt tcgctccggt gattcatggt attgcccgcg gcaccgcaca agtgagtgta
    18781 aaacagaatg gatacgatgt ttatcagact actgtcccac ccggcccttt tactattgat
    18841 gatatcaact ctgcggccaa tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc
    18901 agtattcaga cattatatgt tccttattcg tctgttccgg ttctccaacg tgctggatat
    18961 acgcgttatg cgcttgccat gggggaatat cgtagtggaa ataacctgca aagctccccc
    19021 aagttcatac aaggtagctt gatgcatgga ctggaaggaa actggacacc ttatggcgga
    19081 atgcaaattg cagaagatta tcaggccttc aaccttggta ttggtaaaga tttaggactt
    19141 tttggtgcct tttctttcga tatcacgcag gccaatacga cacttgcaga tggcacccgt
    19201 cacagcgggc aatcgattaa atccgtctac agcaaatcct tctaccagac gggaaccaat
    19261 atccaggtcg caggatatcg ctattctacg caaggttttt ataacttatc cgacagtgcc
    19321 tacagtcgaa tgagtggtta caccgtcaag cctcctaccg gagacagcaa cgagcagaca
    19381 caatttattg attattttaa tctgttctac agtaagcgtg gtcaggaaca aataagcatc
    19441 tctcagcagc ttggaaatta cggtacgaca ttttttagtg ccagtcgcca aagttactgg
    19501 aacacgtcac gcagcgacca gcaaatatca tttggattaa atgtgccgtt tggtgatatt
    19561 acgacttcgc tgaattacag ctattccaat aatatatggc aaaacgatcg ggatcattta
    19621 cttgctttta cgcttaatgt tcccttcagt cattggatgc gtacagacag tcagtcggca
    19681 tttcgtaatt caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg catgaccaat
    19741 ctatcgggag tttatggcac tctgctgccg gataataacc tgaattatag tgttcaggtc
    19801 ggtaacaccc acgggggtaa tacatcgtct ggcaccagtg gttacagttc tcttaattat
    19861 cgtggagctt acggtaatac gaatgtcggt tacagtcgga gtggtgacag cagccagatt
    19921 tattacggaa tgagtggtgg cattattgct catgctgatg gcatcacctt tggacagccg
    19981 ctgggcgaca caatggttct ggttaaggct cctggcgctg ataatgtcaa aatagagaac
    20041 cagaccggta ttcataccga ctggcgtggc tatgccatat taccatttgc gacagaatat
    20101 agagaaaatc gtgtcgctct taacgcgaat tcccttgcag ataatgttga actggatgaa
    20161 accgtggtca ctgtcatccc aactcacggt gctattgcca gagcaacatt taatgcacaa
    20221 atcggcggga aagtattaat gacgttgaag tacggtaata agagcgttcc attcggtgca
    20281 attgtcactc acggaaagaa taaaaatggc agcattgtcg cggaaaacgg tcaggtttat
    20341 ctgactggac ttccacagtc agggaaatta caggtttcat ggggcaaaga taaaaactca
    20401 aactgtattg tcgagtacaa gcttcctgaa gtttctcctg gtaccttact gaaccagcag
    20461 acagcaatct gtcgctaagg aacaatagat tatgaaatac aataacatta ttttcctcgg
    20521 tttatgtctg gggttaacca cctattctgc tttatccgca gatagcgtta ttaaaattag
    20581 cgggcgcgtc ctcgattatg gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga
    20641 tctccaaaaa aacagtgcca gacaatttcc aacgaccggt agcacaagtc cagccgtccc
    20701 ttttcagatt acgttaagtg aatgcagtaa agggacaacg ggggttcggg ttgcatttaa
    20761 cgggattgag gatgcagaaa ataatactct gttgaaactg gatgaaggaa gcaatacggc
    20821 ctccggtttg ggtatagaaa tactggacgg aaatatgcgt ccggtgaaat tgaatgacct
    20881 tcatgccggg atgcagtgga tcccactggt accagaacag aacaatattt tgccttactc
    20941 cgctcgtctg aagtcaactc agaagtccgt caatccggga ctggtgaggg cttcggcaac
    21001 ctttaccctt gaatttcaat aatcagagtc agatatgaaa agacttcaca agaggttcct
    21061 gttagctacg ttttgcgcgt tattcacagc aactctccag gccgccgatg tcactatcac
    21121 tgttaatggt cgggtagtcg ctaaaccctg cactattcaa accaaagaag ctaacgttaa
    21181 tctcggggat ctttatacgc gcaatctgca acaacctggt tctgcatcag gctggcacaa
    21241 tattactttg tcattaaccg attgtccggt tgaaacaagt gcagtgacgg caatcgtgac
    21301 aggttcaact gacaatacgg gttattacaa aaatgaaggt actgccgaaa atattcagat
    21361 agagctaagg gatgaccagg atgcgacgtt aaaaaatggc gatagcaaaa cggttattgt
    21421 tgatgagatc actcgtaatg cacagtttcc acttaaggca agagctatca cggtgaatgg
    21481 aaacgcaagc cagggaacga tcgaggcgct aatcaatgtg atctacacct ggcaataacg
    21541 tcgattcgat tttcttagcc tctttttaaa tcaatttttt aaccttagga tatatcatgg
    21601 gtaaaacaat aagtatgaag cttctgttcg gtatttatct tctattaatg gcaggcaaag
    21661 tttttgcctt cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt acaacctctg
    21721 tttatgttaa tctcgaccct gtaatacaac cgggccagaa tctggttgta gacttgtctc
    21781 agcatataag ttgctggaat gattacggcg gctggtacga cactgatcat ataaacctgg
    21841 tacaaggctc agctttcgca ggctctctac agtcatataa aggatcgctg tactggaata
    21901 atgtgaccta ccccttcccg ttgacgacaa atactaatgt attagatatt ggtgataaga
    21961 caccgatgcc attaccgtta aaactctata ttaccccagt cggtgcggcc ggtggagttg
    22021 tcataaaagc cggggaagtt attgcccgta ttcatatgta taaaattgcc acgttaggaa
    22081 gtggaaatcc gcgtaatttt acgtggaata ttatttcaaa taacagtgtt gttatgccaa
    22141 caggcggctg caccgttgat agtcgtaatg tcaacgtgaa tctcccggac tttcctggaa
    22201 gcgcagaaat accccttggc gtatattgct caagtgaaca aaagctctca ttttatcttt
    22261 cgggtgctac cactgacagt gccagacaag tgtttgctaa cacagcacca gatgcaacaa
    22321 aagcatcggg agtgggtgtc tctctgatgc gtaatggaaa aatactggct acgggagaaa
    22381 acgtctccct ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg
    22441 gacaaacagg aaacaaggtt gcagccggaa ccgtccagtc agtcattggt gtcactttta
    22501 tctatgaata attgtgattt tgacattggt ctatgcgctc cattcacgaa gattgcgcgg
    22561 ataattattt tgtgaaggct attatcctac atctgtaaga aaatccgcgc acaattgaag
    22621 ccagagtctt attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc
    22681 ttaatttatc gtatttaatc ttttgtttaa cgataataga ttaaaataga aagagagtag
    22741 tcccctactc ttatatatcc atgttgacga taatcccttt gtatatactg tgcatcatcg
    22801 ctattacaaa tcctaataat tcatttccac acaggataag caaatgaaga aaaaaattga
    22861 atcctaccag ggagctgccg gtggttgggg tgctgttaaa tccgtagcga atgcagtacg
    22921 taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata agccagaggg
    22981 ctttgactgt ccgggatgtg catggccaga tcctaagcac agtgcgtcat tcgacatttg
    23041 tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa atgcctcttt
    23101 ctttgctgag aatacggttc aatcattact taactgggga gatcacgagc ttgaggctgc
    23161 ggggcgtctc acccagcctt tgaaatatga tgccgtcagc gactgttaca agccattaag
    23221 ctggcaacaa gctttcgacg aaattggcga acgccttcaa agctatagtg atcccaatca
    23281 ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt atcagctttt
    23341 tgctcgtgaa tacgggagca ataacttccc cgactgctcc aacatgtgcc atgaaccgac
    23401 cagtgtgggt ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc tggaagactt
    23461 tgagaaatgc gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat
    23521 gttgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct
    23581 acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa
    23641 ctctgagact cagttggcca gtgcctacta caacgtgcgc attggtggtg atatggcgtt
    23701 gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg ccgcaggtcg
    23761 gccctcattg ctggatgacg aatttattca aacgcatacc gtcggctttg acgagctacg
    23821 ccgtgacgtt ctcaattccg agtggaaaga tatcgagcgt atttctggac taagtcagac
    23881 tcaaatcgcc gaactggctg atgcatatgc cgctgccgaa cgcaccatta tctgttacgg
    23941 aatggggatc actcagcacg aacatggtac ccagaatgta cagcaactgg tcaatctgct
    24001 gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gaggtcactc
    24061 taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc
    24121 tcgcctgggt gagcgctatg gtttcacccc acctcatgca cctggacatg cagcaattgc
    24181 cagcatgcaa gcaatatgta cggggcaggc tcgagcattg atctgcatgg ggggcaactt
    24241 tgcactggca atgccagatc gggaagcaag cgctgtaccg ttaacacaat tagatctgac
    24301 ggtacacgta gccactaagc ttaaccgctc tcatctgctg accgcacggc atagctatat
    24361 tctgccggtt ctgggacgta gcgagattga catgcaaaaa agcggtgcgc aggcggtaac
    24421 cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac ccgccggtgt
    24481 aatgctgaaa tcagagtgtg cagtggtcgc gggtatcgcg caggcggcac taccccagag
    24541 cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc
    24601 tgtgctgcca gagttcgccg actataacca gcgcatccga catcctggag gttttcacct
    24661 gataaacgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta atttcattac
    24721 cagtaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctag tcatggcgac
    24781 agtacgcagt cacgatcagt acaacacgac gatttatggt atggatgatc gctatcgagg
    24841 ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt
    24901 aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc gaagctcacg
    24961 ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta
    25021 ttttccagaa tcgaatcaca tgctaacact tgataaccac gatccattaa gtggcattcc
    25081 tggctataaa agtattccgg ttgaattaga accatcaaat taattcgcct cctcatttct
    25141 tctgctgtca tccgcacagc agaagaattc ctcattgacc attgtcgcgc aatttactca
    25201 catgtgctaa attaaactgc tcgttataag atatatacct ccgcctacag ctgtaagaaa
    25261 ctccgctcag tactgaagcg ccagtcctat ttcctctatt ctccagcctg ttatattaag
    25321 catactgatt aacgatgatt aacgtcattc gtttaataaa cacatttgaa atgcatgcga
    25381 ccatagtgaa aaataaaatc acgcaaagag acaactataa agggatcatg tctgtaattg
    25441 tggttgtctt attactgacc cttacattga tagccatttt ttcggcaatt gatcagctgg
    25501 gtatttcaga aatggggcgc attgcaaggg atcttacaca tttcattatc aatagtttgc
    25561 aagactgaaa ataaacagca aattataaat atgaaatgtt aaaaaagtat cgataaaaac
    25621 tttattgttt taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc
    25681 cttcaacgct aacattttag ataaagatta cgctttctct gacggcgaga tcctgatggt
    25741 agataacgct gttcgtacgc attttgaacc ttatgagcgg catttcaaag aaatcggatt
    25801 taatgaaaat accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc
    25861 tgttcctgcg aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat
    25921 tgcttatctc aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc
    25981 ttgcctgtct atttttgcca catgcaaagg tttcattaca ctattaacta acggtgtgct
    26041 atccgtttcc gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct
    26101 gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca
    26161 agagcaaacg acattctcac agattctttt agatgccaga atgcagcacg caaaaaattt
    26221 gatacgcgta gaaggttcag tcaataaaat tgccgaacaa tgtggttatg cgagtacatc
    26281 ttattttatt tatgcgttcc gcaaacattt tggcaacagt ccaaagagag tttctaagga
    26341 gtaccgtagt caaaggcaca cgggtatgaa taagggcaac acgatgaatg ctttagctat
    26401 ttgattattt gctaacgagt agtcaaccac gcaccctacg taaaaattaa aatggggcag
    26461 ccattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatt
    26521 catcacatat ttatgcattt gcataacctg ttgcatgatt atttatgatc tc
//
LOCUS       DAYPHN010000059        26566 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000059 DAYPHN010000000
VERSION     DAYPHN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.059"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(147..1739)
                     /gene="lsrK"
                     /locus_tag="ACG0BW_002298"
     CDS             complement(147..1739)
                     /gene="lsrK"
                     /locus_tag="ACG0BW_002298"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFK7259250.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1818..2771)
                     /gene="lsrR"
                     /locus_tag="ACG0BW_002299"
     CDS             complement(1818..2771)
                     /gene="lsrR"
                     /locus_tag="ACG0BW_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFK7259251.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISGRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3020..4555
                     /gene="lsrA"
                     /locus_tag="ACG0BW_002300"
     CDS             3020..4555
                     /gene="lsrA"
                     /locus_tag="ACG0BW_002300"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFK7259252.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTQVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSIGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4549..5577
                     /gene="lsrC"
                     /locus_tag="ACG0BW_002301"
     CDS             4549..5577
                     /gene="lsrC"
                     /locus_tag="ACG0BW_002301"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFK7259253.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            5577..6569
                     /gene="lsrD"
                     /locus_tag="ACG0BW_002302"
     CDS             5577..6569
                     /gene="lsrD"
                     /locus_tag="ACG0BW_002302"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFK7259254.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITALVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6581..7603
                     /gene="lsrB"
                     /locus_tag="ACG0BW_002303"
     CDS             6581..7603
                     /gene="lsrB"
                     /locus_tag="ACG0BW_002303"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFK7259255.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKIAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNGKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7630..8505
                     /gene="lsrF"
                     /locus_tag="ACG0BW_002304"
     CDS             7630..8505
                     /gene="lsrF"
                     /locus_tag="ACG0BW_002304"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFK7259256.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8529..8819
                     /gene="lsrG"
                     /locus_tag="ACG0BW_002305"
     CDS             8529..8819
                     /gene="lsrG"
                     /locus_tag="ACG0BW_002305"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFK7259257.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8876..9634
                     /gene="tam"
                     /locus_tag="ACG0BW_002306"
     CDS             8876..9634
                     /gene="tam"
                     /locus_tag="ACG0BW_002306"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFK7259258.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMVAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIINWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9638..10552)
                     /locus_tag="ACG0BW_002307"
     CDS             complement(9638..10552)
                     /locus_tag="ACG0BW_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFK7259259.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAQYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDETEIPAKILPDRHYQLT"
     gene            complement(10749..12200)
                     /gene="uxaB"
                     /locus_tag="ACG0BW_002308"
     CDS             complement(10749..12200)
                     /gene="uxaB"
                     /locus_tag="ACG0BW_002308"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFK7259260.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDVILEKGMREAVRPLC"
     gene            complement(12427..13845)
                     /locus_tag="ACG0BW_002309"
     CDS             complement(12427..13845)
                     /locus_tag="ACG0BW_002309"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFK7259261.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVAFLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13984..14343)
                     /locus_tag="ACG0BW_002310"
     CDS             complement(13984..14343)
                     /locus_tag="ACG0BW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFK7259262.1"
                     /translation="MQSLDPLFARLFRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(14343..15269)
                     /gene="glsB"
                     /locus_tag="ACG0BW_002311"
     CDS             complement(14343..15269)
                     /gene="glsB"
                     /locus_tag="ACG0BW_002311"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFK7259263.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVANYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGHSVY"
     gene            complement(15333..16721)
                     /gene="sad"
                     /locus_tag="ACG0BW_002312"
     CDS             complement(15333..16721)
                     /gene="sad"
                     /locus_tag="ACG0BW_002312"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFK7259264.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANNSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16822..17697
                     /locus_tag="ACG0BW_002313"
     CDS             16822..17697
                     /locus_tag="ACG0BW_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFK7259265.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAQFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            17781..18896
                     /gene="yneK"
                     /locus_tag="ACG0BW_002314"
     CDS             17781..18896
                     /gene="yneK"
                     /locus_tag="ACG0BW_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFK7259266.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PIINPGVPIKRAHTPHIEPLHPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFGNKEPNLLTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQD
                     FPHFQDLFLFFANMLLHYHQFMNPNESDLIDVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            19046..20236
                     /gene="ydeA"
                     /locus_tag="ACG0BW_002315"
     CDS             19046..20236
                     /gene="ydeA"
                     /locus_tag="ACG0BW_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFK7259267.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGAFITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20261..20926)
                     /gene="marC"
                     /locus_tag="ACG0BW_002316"
     CDS             complement(20261..20926)
                     /gene="marC"
                     /locus_tag="ACG0BW_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFK7259268.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21138..21572
                     /gene="marR"
                     /locus_tag="ACG0BW_002317"
     CDS             21138..21572
                     /gene="marR"
                     /locus_tag="ACG0BW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFK7259269.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21592..21975
                     /gene="marA"
                     /locus_tag="ACG0BW_002318"
     CDS             21592..21975
                     /gene="marA"
                     /locus_tag="ACG0BW_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFK7259270.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22007..22225
                     /gene="marB"
                     /locus_tag="ACG0BW_002319"
     CDS             22007..22225
                     /gene="marB"
                     /locus_tag="ACG0BW_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFK7259271.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTTQQVVTSCANVVVVPPSHEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22256..23155)
                     /gene="eamA"
                     /locus_tag="ACG0BW_002320"
     CDS             complement(22256..23155)
                     /gene="eamA"
                     /locus_tag="ACG0BW_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFK7259272.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            23350..24537
                     /gene="ydeE"
                     /locus_tag="ACG0BW_002321"
     CDS             23350..24537
                     /gene="ydeE"
                     /locus_tag="ACG0BW_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFK7259273.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFACGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETASAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRTL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTNLPPSSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            24664..24759
                     /gene="mgtS"
                     /locus_tag="ACG0BW_002322"
     CDS             24664..24759
                     /gene="mgtS"
                     /locus_tag="ACG0BW_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFK7259274.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            24759..24863
                     /gene="mgtT"
                     /locus_tag="ACG0BW_002323"
     CDS             24759..24863
                     /gene="mgtT"
                     /locus_tag="ACG0BW_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFK7259275.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGKKKPPVNRR"
     gene            complement(24978..25868)
                     /gene="dgcZ"
                     /locus_tag="ACG0BW_002324"
     CDS             complement(24978..25868)
                     /gene="dgcZ"
                     /locus_tag="ACG0BW_002324"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFK7259276.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGIRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(26123..26515)
                     /gene="ydeI"
                     /locus_tag="ACG0BW_002325"
     CDS             complement(26123..26515)
                     /gene="ydeI"
                     /locus_tag="ACG0BW_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFK7259277.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         6761 a         6713 c         6379 g         6713 t
ORIGIN      
        1 gaaaaatagc aatttcccct tgaatatatt acgggcgatg gaacgatgaa tctgcaagct
       61 ggcatttaac aaaccagctc taaaagaagg gaaataagaa tactcaaaaa acagccacgg
      121 tcatcatgat gtggctgtca atgaaactat aacccaggcg ctttccataa cgacgtcgtc
      181 agtccatgat caaccagccc cagctgatcc tgataaactg cctgccactt atcccgtgaa
      241 tcctgataaa gttcatgctt ttccgggtct ggtgtgtgcg tccgttccca gcgaaccagg
      301 cgttctccgg tttctgccat tgatgaaaaa attccggcac cgacgccagc tgcaatggca
      361 catcctaatg cggtggcttc tttgaccacc ggaatattga cgggtaatcc cgagacatca
      421 gcgagaattt gactccataa tttccctttt gaacctccgc ctgcaaagac taacgatgaa
      481 ggatgaatat tcgagaaatc agcgatttgt tgcaaattac acgctgatac aatcgccgca
      541 ttttcttcca gcgcacggaa caacgtcgct ttgttacatt tatccggatc aatagacaaa
      601 ttaataaagg aaggcgcagc gtgataccag gttttaaagc gcattctgtc ggagaatatc
      661 ggcattacgc cccacgaccc aggcggcacc cgacttgcca tctcttccag cagcgtatag
      721 gtgtcgatgc ctaaacgttc ggcaatcagt ttttcttcgg cacagaaagc atcgcggaac
      781 cagcgcatgg tgagtccggt aaaaaagctt atagattcag cttgtaccat gccaggaata
      841 acatgaggat taacgcgcac gttcatttct gggtctgtca ccggcgcggc taagtttaca
      901 acttgctgcc agaatgtgcc gccaagaaca gcggtttgtg ccggacgcac aacgcctaac
      961 cccaggcaac caagttgcac gtcgcctcct ccaacgacca ccggagtgcc cgccttcaga
     1021 ccgcagagtt ccgccgcttg tgaacttacc acgcccagca atgtgccggt ttctttgaca
     1081 ggagaaagaa tatcggcccg cagtccggcc atatccagca atgcaggttt ccagtcacgg
     1141 gtggttaggt caagaagtcc cgtggtgcca gcgttagagg gatccaccgc cagttcgccg
     1201 ctgagcatat aggccagcca gtcgctgatc atggtgatgg tcgacgcctg tcggtagata
     1261 tcagaacgat ggtgcgccag ccaaagtaat ctgggaatag cgcttaaagc cagcgtttgt
     1321 ccggtggcgc gataaacttc gttttcaaag gtattgttgt gcagttcttt aagttcgcta
     1381 acttcgcgcg ccgctctggc atccacattg gcgcaggccc agatcggtgt tccttcattg
     1441 ttatataaaa caatgccttc gcgcatcgaa catgccgaaa cggcagcgat atactccggg
     1501 gctatgccgg cgttgtgcag cgcctggcgc atacactcac acgccagttg ccagtttttg
     1561 ttgagatcaa actccataga accaggaacg tccggtactg ccaggtgccg ccactccgcc
     1621 tgtcccactg ctatttgatt gccttccagg tcgaatatca cagcccgaat acttccggtg
     1681 cctgcatcca gcgccatcag gtagtacttt gattctgaag gggtaaagag tcgagccatt
     1741 agattatcct cggcttatgc gttctgtctg ttcctctgta cgttctccat cattcccggt
     1801 aataaggtca tgcaaattta actacgtaaa atcgccgctg cagtatcctg atcggtaacc
     1861 agtgcgttga tataaccgcc tttcattgca gcggcaatgg cttcggcttt attttctccc
     1921 cctgccacgc caacccggac gggaatagtc ttcagcgcgc ttaaaggtaa gccaatcagt
     1981 tcgttatgta ttttgatatc cgtgacaacg tcaccttttg catcaaaaaa gtagcctaaa
     2041 atgtcgccaa cagccccttt tcgaccaatc attaactgtt cgccctggct gatatagccg
     2101 gagcgaatga ttgttgcgtc gtcttgttga ctcacagccc caatgccgac aatcgccaca
     2161 tccgctgctt gcgcggctag cagaacatct ttgacgcaat tttcattttt tagcgtacgg
     2221 gcaatgtcag cggaggatgc ccgcaacgga gccggaataa tattcacact gcacgccgcg
     2281 ttaagctgcc cgatacccgt catataagaa ccgacgccac cggagagcgt gaccaggcga
     2341 atttgctgtg acgaaataaa accacttaag cgttgcagcg tattcatggt tgcctcgcca
     2401 aaaccaatcg ccagcatctg ttgtggttga agtaaactca tcaacatatg cgccgcgcct
     2461 atccctagtc gcccgccgac atcagcatcc gcaagccctg ggatcacccg gacatgttgc
     2521 agcgaaaact gacgacgtaa ttgtgtttca tattccagac agccttcaaa gcgagaatta
     2581 atctgtacgc gaataatgcc ggattgatgc cctttctcca gtaatcgcga cactttcaaa
     2641 cgggtcaggc cgagacgacc gctgatctca ctctgggtca gcccgtcgtg gtagtaaaac
     2701 cacgcaatcc gcgcgacctg ttcttcttca cacattccct gttctgaaat taccgaatcg
     2761 ttgattgtca taattcattc ttcactttga acatacttaa atctttaatg caattgttca
     2821 gttcttgcgc atttatatct gtgatggcaa ccacagtttg actctacgag catgaacaaa
     2881 cgcaaccgtg aaaatcaaaa tagcataaat tgtgatctat tcgtcggaaa tatgtgcaat
     2941 gtccacctaa ggttatgaac aaattaaaag cagaaataca tttgttcaaa actcacctgc
     3001 aaaactgaac gggggaaata tgcaaacgag tgatacccgc gcgttaccgc tactttgcgc
     3061 ccgctcggtt tataaacagt attcaggggt taatgtcctg aaaggcatcg attttacgtt
     3121 gcatcagggg gaggtccacg ccctgctcgg cggcaatggt gccggtaaat cgacgttaat
     3181 gaagattatt gccggtatta cccctgctga tagcggtacg ctggagattg ggggcaacaa
     3241 ctacaccaga ttaacgccag ttcatgctca tcagctgggt atttatctcg ttccccagga
     3301 accgctgctt ttcccaagcc tgtcgataaa agaaaacatc ttgtttgggc tggcaaaaaa
     3361 acagctctcc atgcagaaaa tgaagaactt gctggcggcg ctgggctgcc agtttgatct
     3421 gcatagtctg gcaggctcac tggatgttgc cgatcgccaa atggtggaaa tcctccgcgg
     3481 gctgatgcgc gactcgcgga ttctgatcct cgatgaacct accgcctcgc ttacccccgc
     3541 cgaaaccgaa cgtttgtttc gtcgcttgca agagctgctt gctactggcg tgggtattgt
     3601 ttttatctcg cataagctgc cggaaattcg ccagattgcc gatcgaatta gcgtgatgcg
     3661 cgacggaacc atcgccttaa gcggcaaaac cagcgaactg tctaccgacg acattattca
     3721 ggccatcacg ccagcgctac gggaaaaatc gctctctgcc agccaaaaat tatggctgga
     3781 attacctggc aaccgcccac aacatgccgc cggaacgcag gtgctgacac tggaaaatct
     3841 gaccggcgaa ggtttcagga atgtcagcct gacgctcaat gccggagaaa ttctgggcct
     3901 ggctgggctg gtgggagccg gacgcacaga actggccgag acgctctatg gtctgcgtac
     3961 tttgcgtggc ggacgcatta tgctgaatgg taaagagatc aatagattat ccatcggaga
     4021 acgtttactg cgcggtctgg tttatctgcc ggaagatcgc cagtcatccg gactgaatct
     4081 cgatgcttca ctggcgtgga acgtctgcgc cctcactcat aaccttcgtg gattctgggc
     4141 gaaaaccgcg aaagataatg ccacgctgga acgttatcgt cgggcgctga atattaaatt
     4201 taaccaacca gaacaagctg cacggacctt atccggcggc aaccagcaaa aaatcctgat
     4261 tgccaaatgc ctggaagctt cgccgcaagt attgattgtc gatgagccga cgcgcggcgt
     4321 ggatgtctcg gcgcgtaatg atatctacca actgttgcgc agcatcgccg cacagaatgt
     4381 ggctgtgctg cttatctcct ctgatctcga agagatcgaa ctgatggcag atcgcgtgta
     4441 tgtgatgcat cagggcgaaa ttgcccactc tgcactgacc gggcgcgata ttaatgtcga
     4501 gaccattatg cgcgttgcct tcggcgatag tcagcgtcag gaggcgtcat gctgaaattt
     4561 attcagaaca accgtgaaat cacggcactg ctggcggtgg tgctgctgtt tgtattaccc
     4621 ggttttctcg accgtcagta tttaagtgtg caaacgctga ccatggttta tagcagcgcg
     4681 caaatcctga tcctgctggc aatgggcgcg acgttggtga tgctcacgcg caatatcgat
     4741 gtttcggtag gttcgattac cggaatgtgc gcagtgctgt tgggaatgtt actgaacgcg
     4801 gggtattcac tacctgttgc ttgtgtcgcg actttactgc ttggtttgct cgcgggattt
     4861 ttcaacggtg tcctggtcgc gtggctaaag atccctgcca ttgtcgccac cctcggtaca
     4921 ttaggattgt acaggggcat catgttgctg tggactggcg gtaaatggat tgaggggtta
     4981 cccgccgaac tgaaacaact ctccgccccg ctactgtttg gcgtttcagc aattggttgg
     5041 ttgacgataa ttctggtggc atttatggcc tggctgctgg cgaagacggc gtttggacgc
     5101 agtttttatg ccacgggcga taatttgcag ggcgctcgtc aactgggcgt tcgtacggaa
     5161 gccattcgca ttgtggcatt ttcgttgaac ggctgcatgg cggcactggc ggggattgtg
     5221 tttgcttcgc agattggttt tatccccaac cagaccggta ccgggctgga gatgaaagca
     5281 attgcagcct gcgtgctggg cggcattagt ttgctcggcg gttccggcgc gatcatcggt
     5341 gcggtactcg gcgcatggtt cctgacgcag atcgatagtg tactggtgct gttgcgcatt
     5401 ccggcatggt ggaatgattt tatcgcgggc ctggttctgc tggcggtgct ggtgtttgat
     5461 ggacgcctgc gttgtgcgct ggaacgtaat ctgcggcggc aaaaatatgc ccgctttatg
     5521 atgccaccgc catccgttaa acccgcttcg tcaggtaaaa aacgggaggc cgcgtaatgc
     5581 gtattcgcta cggttgggaa ctggctcttg ccgcactgct cgttattgag attgttgcat
     5641 ttggtgcaat taacccgcgg atgttagatc tcaatatgtt gctgttcagc accagtgact
     5701 ttatctgcat tggcattgtc gccctaccgc tgacgatggt gattgtcagt ggtgggatcg
     5761 atatttcgtt tggttcgacc atcggcctct gcgccattgc attgggcgta ttgtttcaaa
     5821 gtggtgtgcc gatgccgctg gcgattctcc tgaccttact gctcggcgca ttgtgcgggc
     5881 tgatcaacgc cggattaatt atctatacca aagttaaccc gctggtgatt acgcttggca
     5941 cgctgtatct gtttgccgga agcgccctgc tgctttccgg gatggccgga gcgacggggt
     6001 acgaaggtat tggtggattc ccgatggcgt ttacagattt cgctaacctg gatgtactgg
     6061 gactccccgt tccgctgatt atcttcctga tatgtctcct cgttttctgg ctctggctgc
     6121 ataaaaccca tgccggacgt aacgtgtttt taattgggca aagcccgcgt gtggcggttt
     6181 atagcgcgat tccagttaac cgcaccttgt gtgcgctcta tgccatgacg gggctggcgt
     6241 ctgcggtcgc cgctgtgctg ctggtatcgt attttggttc agcacgttcc gatctcggtg
     6301 cgtcgtttct gatgcccgcc atcaccgccc tagtgctcgg cggtgccaat atttatggtg
     6361 gttctggttc cattatcggc accgccattg cggttttatt agtgggatat ttgcaacaag
     6421 gtttgcaaat ggcaggagtg ccaaatcagg tgtccagcgc cctttccggt gcgctactta
     6481 tcgtcgttgt cgtaggtcgt tccgttagcc tgcatcgcca gcaaattaaa gagtggctgg
     6541 cgcgtcgggc caataaccca ttgccataaa ggatatcttc atgacacttc atcgctttaa
     6601 gaaaatcgcc ttacttagcg cgcttggcat tgccgcaatc tctatgaatg tgcaggccgc
     6661 agagcgtatt gcatttattc ccaaactggt tggcgtggga ttttttacca gcggtggcaa
     6721 cggcgcacaa caagcgggta aagagctggg cgttgatgtg acctacgacg ggccgacaga
     6781 acccagtgtt tctggtcagg tacagttgat taataacttc gtcaatcagg gctataacgc
     6841 cattatcgtt tctgcggttt cgcctgatgg cttgtgtcct gcactgaaac gcgccatgca
     6901 acgtggtgtg agagtgctga cctgggactc tgataccaaa ccggagtgcc gctcttacta
     6961 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     7021 ggtgaataaa gacaaagcca aaatcgcgtt tttctactca agccccaccg ttacggacca
     7081 aaaccagtgg gtgaaagagg cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     7141 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     7201 attaaaagcc tatagcgatc tcgacgccat tatcgccccc gacgccaacg ccctgcccgc
     7261 tgccgcacaa gccgcagaaa acctgaaaaa tggcaaagtt gcgattgtcg gattcagtac
     7321 gccaaacgtg atgcgtccgt atgtggaacg cggcacggtg aaagaatttg gcctgtggga
     7381 tgtggttcag caaggcaaaa tttcagttta tgtcgcggat gcattattga aaaaaggatc
     7441 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggtggaag tctcgccaaa
     7501 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7561 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7621 gagtacgtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7681 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7741 tcacgcttat cacggatatt taatccgaaa acgggtaaaa ccgtgatgct ggcctttgac
     7801 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7861 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagtcccc
     7921 ccggcgacca ataagccggt ggtactacgg gcgtcaggtg caaactctat tctggcggaa
     7981 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     8041 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     8101 caactggtcg atgccggaat gaaagcggga atgcccacca tggccgtgac aggcgtgggc
     8161 aaagatatgg tgcgcgatca gcgttatttc tctctcgcga ctcgaatcgc cgctgaaatg
     8221 ggggcgcaga ttatcaaaac ttattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     8281 tgtccggtac ccattgttat tgctggcggt aaaaaactac cggagcgcga ggcgcttgaa
     8341 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     8401 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     8461 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     8521 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgacgagt
     8581 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggc aatttgcgct
     8641 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatctacgaa gcctataaag
     8701 atgaagacgc agtggcattc cataaaacca cgccccacta caaaacctgt gtcgcgaaac
     8761 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8821 gcgaatttat caattttatc tacaattggg gtaacgcgct gacgggagta aaaaaatgtc
     8881 tgactggaac ccctctttat atctgcactt tgccgctgaa cgatcgcgtc cggcggtgga
     8941 gctgcttgcc agagtgccgc tggagaatgt cgaatatgtt gccgatcttg gctgtggccc
     9001 aggtaacagc accgcccttc tacaacaacg ttggcctgct gccaggataa cgggcatcga
     9061 ctcatctccg gcgatggttg ccgaagcacg cagtgctttg ccggactgcc tgtttgtgga
     9121 agcagatatc cgcaactggc aaccggaaca ggcactcgat ctgatttttg ctaatgcctc
     9181 actgcaatgg ctgcccgacc actacgaatt gtttcctcat ttggtttcgt tactcagtcc
     9241 gctgggcgtg ctggcagtac agatgccaga taactggctg gagccgaccc atgtgctcat
     9301 gcgcgaagtt gcctgggaac aaaactaccc ggatcgcggg cgtgagccgc tggctggcgt
     9361 tcatgcttac tacgatattc tgagtgaagc cggatgtgag gtcgatatct ggcgaaccac
     9421 ttactatcac cagatgccgt cgcaccaggc gattatcaat tgggtgaccg ccactggatt
     9481 acgtccgtgg ttacaggatc tgaccgagag cgaacagcag cattttctta cgcgctacca
     9541 tcagatgctg gaagagcaat atcccctcca ggagaacgga cagatattgc tggcatttcc
     9601 gcgtctgttt attgttgccc gccgtacgga gtaattatca cgtcagctgg taatggcgat
     9661 cgggaagaat tttcgctgga atttctgttt catcgttcat ctgtaacagg tcaatttcaa
     9721 tagcgttgca gatggcatcc agcggtaaat cattgttttc agtaccgaag ggatcttcca
     9781 gttcttccgc cagacaatcc agcgaaataa aagtgtagga aatcagcaca gagataaaag
     9841 gcgtcatgta atgcagatcc acgaccagcg cgaacggcag catgatacaa aacagataaa
     9901 cggtacgatg caaaatcagg gtgtaggcaa agggaattgg cgtataggca atgcgctcgc
     9961 agcctgccag aaccgctgaa atatcattaa gccgatcgtt gaggctaata aacaggatat
    10021 ctgaaagctg tccattgcgg cgctgaaccg ccaaccactc ccccattatt aacaagatac
    10081 ggttagctgg agagttcgaa gccagtacac gctgaagatc ttccgtcttg agatattgag
    10141 ccagcacttc cgcctgtggt tgtttgcgta atgtcatgcg taaacagtga gcgaaggcga
    10201 tttgcagccg ggcaaactcc cttacacttg ccgaatctgg caatgtcgtt tttacctccc
    10261 gcagtaacga ccgtgaggca atcatcaact gcccccaaag ttttcgcgct tcaacgtaac
    10321 gggcgtaccc ggcattatta cgaaaaccaa gaaaaatggc gatggcgaca ccgagaatgc
    10381 tgaacggtgc gagggtgaat ttgatgccca gatgcgtgta ccaaggcagc atgaaaataa
    10441 cagcgataga aaagagaaaa ttgagtagta agcgcgagga tatcttggat aatactgagc
    10501 catgccagac aaaaatacgg cgcagccagt gttgttgtgg acgaactatc atggttatct
    10561 tcaggcgtgg aaaagtcgcc ctattaaacg tgattacgat cacattctca agacgctacg
    10621 tacaaattac ctacaagctt acaacattta caggtgtggc atatggcacc cgttaatgat
    10681 aaaggcacat aggatgttgt aactaatata gtgaaataag aaacccggtc gaaaccgggt
    10741 tcagagtatt agcacaacgg acgtacagct tcgcgcattc ctttttcgag aatcacatcc
    10801 aggtcattag ccacctgctc gaccagacca ggcacttgcg tcaggtcctg ctcccagtgg
    10861 tctttttctg ccaaaacaat cgctaccagt tcctgggtac cgataacacg gtcacgatgc
    10921 tgactccaga gttgctgata acgttccagc cagtgtgcat cgtcctgtac cggatatgtt
    10981 tctccgttac gctcaccacg atagaacgca atcaatgccg ctaatgcgaa agtaaggcgc
    11041 gccggaagtg tgccgtttgc cttctgccct gccagcagct gcggcaggat gcgggtgcgg
    11101 aacttggtca taccgttgag cgcgatagac agcagctgat gcttaatgta cgggttacgg
    11161 aaacgcccgg tgactgcact ggcgaaagat tccagttcat cacgaggcaa atccagtacc
    11221 ggaataattt cttcgtagat ggctttttca acgaatgcgc aaatttcagc atcgttcatc
    11281 gcttcaccta cggtatccag ccctgcctgg aacgccaccg gcaccagcgc ggtgtgcgca
    11341 ccgttgagga ttgccacttt gcgttcttta tacggtttaa tatcgtcaac aatcagcacg
    11401 ttgagcggat atttgtccag gcgcagctca gtcgctaagg atttcggccc ctgaatcaca
    11461 aacaggtaaa agtgttcagc ggtgtcgagg aaaccatcgt gataacccag ttcttcttcc
    11521 agtttagcca cttcatcgcg cggataaccg gtaacgatac ggtccaccag cgtagaacag
    11581 aagctgttag cctgatccag ccattgaata aatgcttctg gcagtgccca ctcttgcgca
    11641 tagcgcagca ccagttcacg caacgcgtcg ccattgtagt caatcaactc acacggaatg
    11701 atgatccaac ctttatccag cgcaccgttg aaatggctaa agcgttcgaa cagcagacgg
    11761 gtcagttttg ccggatagct taccgctggt gcgtcatcga atttatcgcc cgcatggtag
    11821 ctgatccccg cttcggtggt gttagagaaa acaaagcgca tttccgggtt gtgcgccagt
    11881 ttcaggaatt catcgtattc actgtagacg ctgatttcac gattaaccga gcgaatcaga
    11941 cgcgcgtcgc tgaccgcttc ccccttctca ttcaggccac ggataatggt ggtgtacaga
    12001 ccatcctgcg tgctcagtga cggcgggaat gaagtttcaa tcggacgaac aacgaccacg
    12061 ccagaattca gatcggtgtg ctcattcagg agatcgattt gccagtcaac aaaggcgcgc
    12121 aggaagttac cttcaccaaa ctgaatgata cgttctggat actgtgcacc gggaaaatcg
    12181 cgacgattta gtgttttcac aatgggttcc cttctgatta gtcatacaac ctgtttgaat
    12241 tggtacgaca ggttagcaaa ctttaatacg ccgaacccct gttttgatca actcctgatg
    12301 attaatgagc agttttatga gaaaagtgtg gcgcggatca tggtttaatc gaggaaatac
    12361 gccttttcct ggatcataaa gtggtagaac acattgcatt caaatcgcgc ggaatgaata
    12421 aagatgtcag acaacttcct caccgtaacg catagtgctg gtacggttgc gcccatcttt
    12481 cttcgagcga tacagacacg tatcggcttc gaccatcaat ttattaaaat catcggtcag
    12541 cgttcggtac gatgcgcgac cactaccgac gccaatactt accgtgagat aaagcgtttt
    12601 ttgttgccag gtgaacggtt gcagttcaac gcctttacgg attttttccg ccattagcag
    12661 accatcgaca ggattcaccg acggcactgc aacagcaaat tcttcgccgc ccatgcgcgc
    12721 caccagcccc ttatcaccga caatcttctg aatatgccgg gcaaacacgc ttaacacttt
    12781 atcgccacat tcatgtccat agttatcgtt aatgcttttg aaatagtcaa tatcaagcag
    12841 catgaccgtc agatgttgtg tctgtttcag cgatggactt ttcagcgcct cataaagacc
    12901 ggaccgtgag taaacctgag tcagaaaatc aaagtcggct cgcagcgcaa cttgcttcat
    12961 tagcgaattg atcgctgcca cgctaaaaga aaccataatt gggcatatcg ccatcgtagc
    13021 aataccgaga cgtgcggaga acatttgcgg aatggagaac ggcgaaccga ccgagatatc
    13081 aatcaccgaa tttgccacca gtacgatttc caccgcaccg gtgacaaagg tcaacagaca
    13141 tgttacctgc ggcgtatagc gcacggcaca ccagattaat gcaggcagcg ggaacgccag
    13201 actccccgct ccgccaatga ctaccgaggc aataaccgac acaataagcg cgatagcggg
    13261 catcatctgt tctgctttaa agcgcggcag cactcccgga atagccaacg tcagcataca
    13321 aggcacgatc aacacgcccg ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca
    13381 gaaatccaga ctatcaatac tgaccgaacc aatcgccccg acaatagcgc ataataatgc
    13441 ggcaatcaga cagtaattaa atagccgtaa ggcacttact ggctcatact tatttttccc
    13501 cagacgctta tcccgcgcga ccagaaaggc gacggtaaca ataaacatca tattggagaa
    13561 attgatagcc agtgaaacca gcccccattc ggtggttatg gcatcataaa ccagcatcgc
    13621 cacataactg atcgcatagt aatgcagtcg attaagccag acatagcggg caaacacccc
    13681 cgccattacg ccattcagcg gccagaaaag agaaagttcg tgaaccaacc gtaactctgc
    13741 accaataaaa tagaacagcg tggttagcgc aaaaatagcg atgctattac gtagcagatg
    13801 accttcctgg aacaaacgga aagttgaaat gggctgtaca tgcattaatt atctgtccat
    13861 acaattgcgg gtggcaatta tctcaacgta ataagtaaat tcttataaag gatagcatgt
    13921 tcgctgacag cggcggcaaa taaagatatc ctgggaatgc tattttatcg ttttccgcaa
    13981 ttatcatggc tgattcatct gtataaccaa ccagtgatag attaccgcca cgatataacg
    14041 ttgttgttcc tcgcttaacg aaacaccctg cggaatgtta tgccacttag ccagacaacc
    14101 gcgacaacag gttgcagtgg cgtgctgagc gataaacacc ggatgcccgc gcatgggggt
    14161 ttgcttaccg tcgttagccg gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc
    14221 atgttgttcg atgactggcg cgcctttctc cagacaatac tgacgctctt tcacccccaa
    14281 acgaaagcga gagcgaaatt ttgaacggaa taaacgcgcg aagagtggat cgagagactg
    14341 cattaataaa ccgaatgccc taactgtttc gtcaattgtt caaggacggc gatacccgcg
    14401 agcgagttac ctgcatcatc cagttccgga ctccagactg cgatggccat ttcatgcgga
    14461 acaatcgcca caataccgcc accaacgcca gatttcgccg gtagtcccac ccgccaggca
    14521 aactcccccg cgttctggta cataccactg gtcgccatca gcgcattaat ttgccgcgcc
    14581 tgcattggcg tcaccacagg ttcatcaata tgaatagctt tcccctgatt agccagaaag
    14641 acaaacgtcc gggccagctc tacacagctc attttcagag cgcagtaatg aaagtagttt
    14701 tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg acttcatcag ccaggcgata
    14761 gccgcatttc gcgcggagtg ttcaaattcg gaacgcgcta ccaccgtatc gtaggaaata
    14821 tcagacacac cgcttaagcc gcgcacgact tccagcatac gttgccgagg agcgcttaat
    14881 cgcccttgca acatatcgca gaccaccagc gcaccggcat taataaacgg attacgcgga
    14941 ataccctgct ccatttccag ttgcactaaa gaattgaacg gtgatccaga cggatctttg
    15001 ccgacgcgtt gccagatttc ctcttcggag taatgacgca tggcgacaac gagactcagc
    15061 actttggaaa tagactgaat ggaaaaacgt tcttgtgcgt ctccggcctg aaaaagctgt
    15121 ccgtcaacgg tacagatagc aatgcccaat cgggaaccgt ctactgtagc cagcgccgga
    15181 atataattcg cgactttacc ctgaccaatg agcggccgta cttgccgcaa gatgttttct
    15241 aaaattgcat tatccatggc gactgccact ttctactcct ggaccgcagg tctgaaaaga
    15301 cctgcgagta tatcagagct gaatatgtag cgtcagatcc ggtctttcca caccgtctgg
    15361 atattacaga attcgtgtaa gccgaaatgg gaaagctcac gaccaaagcc actctttttc
    15421 acgccaccaa aggccactcg cgcgtcgctg gcacaataac cattgataaa caccccaccg
    15481 cattccagac gtgccgccat ctgtctggcc cgggtttcgt cggtggtgaa aatggtcgct
    15541 gaaaggccga actcgctatt attagccagt tccagtgcat gttctgcatc tttcgcaatg
    15601 gtgattgccg caacggggcc aaacatttct tcccgaaacg cggtcatttc tggggtaaca
    15661 ttcgccagaa ccgttggcgg atagtagtta cctgccccag ccatcttttc cccgcccagt
    15721 aacaaacgcg caccctgcgc cagggttttc tccacctgat gatgcagctc atcacgtaaa
    15781 tcaaaacgag ccattggtcc gagagtgttc tcttcgtcac gtggatcgcc cattttcaag
    15841 gctgccgcag ctgccacaaa acgttcggta aatgccgaag caattccctc ttcgatgata
    15901 aagcgttttg ccgctgcaca aacctgtccg gtattctgat aacgtccggc taccgccgct
    15961 ttaaccgcca gttccagatc ggcatcgtta agcacaataa atggatccga accgcccagt
    16021 tccagtacgc attttttcag tgccgctcca gcctgtgcgc caatagccgc tcccgcacga
    16081 acacttccgg tcaccgtgac agcagcaatg cgcgagtctt taatcatctg actgacgccg
    16141 tcgttgtcgg cattcagcca gccatatacg ccttgtggga tacccgcatc tttaaacacc
    16201 tgggcaatga gctgtgcaca gcccatcaca ttcggcgcat gtttaagtaa gtagccgtta
    16261 cctgcaagaa tgatgggaac cgcgccacgc atcacctgcc ataatggaaa attccacggc
    16321 ataatcgcca gaattgtccc caacggtcga tactcaataa ctgcctgctg attttccacc
    16381 agcgtaggtt ccgccttcag cattgccgga ccatgttctg cataccagtc acacaaattc
    16441 gccgatttcg ccacttcagc acgcgcctgg ttgatcggtt tgcccatttc acgggtgatc
    16501 atttgcgcca tttcttcgct acgggcgcgc agagccttac cgatatcacg cagtttttca
    16561 gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc ctgctgccgc cagctgaagt
    16621 gcgttttcga tatcatcagc gccagcccac ggcagcacag aaagttgttc accggtggca
    16681 ggatttatcg aaattgcatg agttgccgga gtaatggtca tcggggtatc tcctttatga
    16741 gttatggtaa aaagatacgc agatttactc ttgcttgaaa atgaataata ttaagccact
    16801 tattcatgaa tcgagaatat tatggatctg acccaactgg agatgttcaa cgcggttgcc
    16861 gaagctggca gcattaccca ggctgcagca aaagtgcatc gcgtgccgtc gaatctcact
    16921 acccgtctac gccagctgga aacagaactg ggggttgatc tgtttattcg cgagaatcag
    16981 cgtttacgtc tctcgcctgc cgggcataac tttttacgct atagccaaca aattctcgcg
    17041 ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc cgcaaggttt attttctctt
    17101 ggttcgctgg aaagcaccgc agcggtgcgc attccggcca cgctggcgca atttaaccgt
    17161 cgttatccca aaattcagtt ttcactttcc accggccctt ccggcactat gctggaaggt
    17221 gtgctggagg gaaaactgaa tgcagcgttt attgatgggc ccattaacca tactgccatt
    17281 gacgggatac cggtgtaccg cgaggagctg atgatcgtca cgccacaagg acatgcgcca
    17341 gtaacccgcg ccagtcaggt taatggcagt aacatttatg ccttccgcgc caattgttcg
    17401 tatcgtcgcc acttcgagag ctggtttcat gctgacggtg ccgctccggg aactatccat
    17461 gagatggagt cttatcacgg aatgttggcc tgtgtggtcg caggagcagg tattgcgctt
    17521 attccgcgct ctatgctgga aagtatgccg gggcatcacc aggttgaggc gtggccgtta
    17581 gctgagcaat ggcgttggtt aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt
    17641 ccgcttgagg catttattca actactggat gtgtctgact cggcaaaaca gagttagcaa
    17701 tgagctattt ttgatagttc ttgcggctaa tatgctctat atagtgatgt tccgatgact
    17761 tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac atatctcttc
    17821 ctgatgattt tcccgtacgt aatattgcac cacaggtaaa agaagtttta aaggatttca
    17881 ttgatgcact cagtacaata atatgtgatg aagagtggcg tacgtcttta aacatcaact
    17941 cagccacaaa aaagatattt aataaccttg acaacttatc atatattcag aggacttctt
    18001 ttcggggtaa cgacacgcta tacaatgaaa aggttcagtt taaactcact tatcccgtca
    18061 agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta agccctattt
    18121 atttagataa tttccgccat gatggggaaa ttaatatttt ttgcgctccc aacccaaagc
    18181 ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtactcttt ctgttcctga
    18241 atgactttat agaacaattt ccaataatca accctggcgt tcctatcaaa agagcgcata
    18301 cgccacatat tgaacccctg catccggatc accataccgc tgcagattac ttacgccagt
    18361 ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc ccccgattat
    18421 ggaataactc tgaggttcac agatggttcg ggaataaaga acctaacttg ctcaccgcta
    18481 ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagtctg atggattcac
    18541 taggttctaa caaacatgta ctacccgaag tctgcatctg ctttttatcc cttttagcag
    18601 aacaagattt tccacatttt caggacttgt ttttattttt cgccaatatg ttattgcact
    18661 atcaccaatt tatgaatccc aatgaaagtg atttgattga cgtgttgatg ccagcatcat
    18721 taagtgatga taaaattatc aaacatatgg cgcgcaagac cctcaaactt tttgtaaaaa
    18781 atgaaacgcc gccaaaagtt actcatgaag acctggtgaa aaacaggcct cgctcccctg
    18841 tcagaccacc tatacccgca acctccaaaa cgccagacct ccctgaacgt cattaaaccg
    18901 tgatgtgacc gactctctga cgcgtgaacg aatcagcgtc agagaaacgg aaaacgcgat
    18961 ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
    19021 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
    19081 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
    19141 agttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
    19201 tatgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta tgttaatgac
    19261 cagccaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
    19321 cgtactgtcg tttttgtcgt ggagctttac cgttctagtg atcagtcgca ttggtgtggc
    19381 ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta tggctccggc
    19441 cggaaagcga gcgcaggcat tgagtttaat tgccaccggt acagcactgg caatggtatt
    19501 aggtttacct ctcgggcgca ttgtggggca gtattttggc tggcgaatga ccttcttcgc
    19561 gattggtatc ggggcgttta ttaccctttt gtgcctgatt aagttacttc ccttactgcc
    19621 cagtgagcat tccggttcgc tgaaaagcct accgctatta ttccgccgcc cggcattgat
    19681 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
    19741 cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
    19801 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcagta
    19861 tgcgtctgcg ttggtaagta cggcgattgc gctgttgctg gtgtgcctgg cactgctgct
    19921 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatagcgat
    19981 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgctaccga
    20041 cgtcgcgatg gcgctattct ctgggatatt taatattgga atcggcgcgg gtgcgttggt
    20101 aggtaatcag gtcagtctgc actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc
    20161 tgcttttgcc gcgttaatat ggtcaatcat cattttccgc cgctggccag tgacactcga
    20221 agaacagacg caatagttga aaggcccatt cgggcctttt ttagtggtac gttttaatga
    20281 tttccaggat gccgttaata ataaactgca cccccataca taccagcagg aatcccatca
    20341 gacgggagat cgcttcaatg ccccccttac ccaccagccg cataattgcg ccagagctgc
    20401 gtagacttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
    20461 gtacccaatc agcaaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
    20521 ctatggttcc cggaccggcg gtacttggca ttgccagtgg tacaaaagca atattggcgc
    20581 tgggttcatc ttccagctct tccgacttgc ttttcgcttc cggtgaatca attgctttct
    20641 gttgcggaaa gagcattcga aaaccgataa acgcgacgat taagccgcct gcaattcgca
    20701 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa tacgccacca
    20761 tcatgatggc aaagacgtac accgaggcca tcaacgactg acgattacgt tcggcactgt
    20821 tcatgttgcc tgccaggcca agaaataacg cgacagttgt taatgggtta gctaacggta
    20881 gtaacaccac cagccccagg ccaattgctt taaataaatc taacattggt ggttgttatc
    20941 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caacgataaa gtgtcactct
    21001 ttagctagcc ttgcatcgca ctgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg
    21061 gtcaattcat tcatttgact tatacttgcc tgggcaatat tatcccctgc aactaattac
    21121 ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
    21181 gtcgcttaat ccatatggtt aatcagaaga aagatcgcct gcttaacgag tatctgtctc
    21241 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
    21301 ttactccggt tgaactgaaa aaagtgttat cggtcgacct gggggcgctg acccgtatgc
    21361 tggatcgcct ggtctgtaaa ggctgggtgg aaaggttgcc gaacccgaat gacaagcgcg
    21421 gcgtactggt aaaacttacc accagcggcg cggcaatatg tgaacaatgc catcaattag
    21481 ttggccagga cctgcaccaa gaattaacaa aaaacctgac ggcggacgaa gtggcaacac
    21541 ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
    21601 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
    21661 tcgccactgt cactggagaa agtgtcagag cgttcgggtt actctaaatg gcacctgcaa
    21721 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
    21781 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
    21841 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
    21901 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaat
    21961 cattacaaca gctagttgat aacgtgacaa cgtcactgag gcaatcatga aaccactttt
    22021 atccgcaatt gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg aacaaaccac
    22081 gcagcaggtt gttacttcct gtgccaatgt cgtggttgtt cccccatcgc atgaacaacc
    22141 accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc
    22201 ctattataat caacacgcta tgtagtttgt tctggccccg acatatcggg gcttattaac
    22261 ttcccacttt taccgcttta cgccaccgca agccaaatac attgatatac agcccggtca
    22321 taatgagcac cgcaccgaaa aattgcagac ccgttaagcg ttcatccagc aatagtgccg
    22381 cacttgccag tcctactacg ggcaccagta acgataacgg tgcaacccgc caggtttcat
    22441 agcgtcccag taacgtcccc cagatcccat aaccaacaat tgtcgccaca aacgccagat
    22501 acatcagaga caagatggtg gtcatatcga tagtgaccag actgtgaatc atggttgcgg
    22561 aaccatcgag aatcagcgag gcaacaaaga agggaatgat tgggattaat gcgctccaga
    22621 ttaccagcga catcaccgcc ggacgcgttg agtgcgacat gatcttttta ttgaagatgt
    22681 tgccacacgc ccaactaaat gctgccgcca gggtcaacat aaagccgagc atcgccacat
    22741 gctgaccgtt cagactatct tcgattaaca ccagtacgcc aaaaatcgct aaggcgatcc
    22801 ccgccaattg tttgccatgc agtcgctcac cgaaagtaaa cgcgccaagc acgatagtaa
    22861 aaaacgcctg tgcctgtaac accaacgaag ccagaccagc aggcataccg aagttaatgg
    22921 cacaaaaaag aaaagcaaac tgcgcaaaac tgatggttaa tccatacccc agcagcaaat
    22981 tcagtggtac tttcggtcgt gcgacaaaaa agatagccgg aaaagcaacc agcataaagc
    23041 gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg accacaaaat
    23101 ttagccccca tacgaccact accagtagcg ccaacacccc atcttttcgc gacattctac
    23161 cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt ttctttaaca
    23221 gttgattcgt tagtcaccct ttacgacggc attaatgcgc aaataagtcg ctatacttcg
    23281 gatttttgcc atgctatttc tttacatctc taaaacaaaa cataacgaaa cgcactgccg
    23341 gacagacaaa tgaacttatc cctacgacgc tctaccagcg cccttcttgc ctcgtcgttg
    23401 ttattaacca tcggacgcgg cgctacgctg ccgtttatga ccatttactt gagtcgccag
    23461 tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac tattggcgtc
    23521 gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg ctatatgtta
    23581 ctggcaatta ccgccttcgc ctgcggtttt attgccattc ctttagtgaa taacgtgacg
    23641 ctggtggtgc tcttttttgc cctcattaac tgcgcctatt ccgtttttgc tactgtgttg
    23701 aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa cgaaaatctt ctcaatcaac
    23761 tacaccatgc tcaacattgg ctggaccatc ggtccgccgc tcggcacgct gttggtgatg
    23821 cagagcatca atctgccctt ttggctggca gctatctgtt ccgcgtttcc catgcttttc
    23881 attcaaattt gggtaaagcg cagcgagaaa atcatcgcca cggaaacagc cagtgcctgg
    23941 tcgccgaaag ttttattaca agataaagca ctgttgtggt ttacctgctc tggctttctg
    24001 gcttcttttg ttagcggcgc atttgcttca tgcatttcac aatatgtgat ggtgattgct
    24061 gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc cagtgaatgc tgccatggta
    24121 gttacgttgc aatattccgt gggccggcga cttaacccgg ctaacatccg cacgctgatg
    24181 acagcaggca ccctctgttt cgtcatcggt ctggtcggtt ttattttttc cggcaacagc
    24241 ctgctattgt ggggtatgtc agctgcggta tttactgtcg gtgaaatcat ttatgcgccg
    24301 ggcgagtata tgttgattga ccatattgcg ccgccaggaa tgaaagccag ctatttttcc
    24361 gctcagtctt taggctggct tggtgcggcg attaacccat tagtgagtgg catagtgcta
    24421 accaacctgc cgccttcctc gctgtttatc atcttagcgt tggtgatcat tgctgcgtgg
    24481 gtgctgatgt taaaaggaat tcgtgcaaga ccgtgggggc agcccgcgct ttgttgattt
    24541 aagtcgaaca caataaaaat ttaattcagc cttcgtttag gttacctctg ctaatatctt
    24601 tctcattgag atgaaaatta aggtaagcga ggaaacacac cacaccataa acggaggcaa
    24661 ataatgctgg gtaatatgaa tgtttttatg gccgtactgg gaataatttt attttctggt
    24721 tttctggccg cgtatttcag ccacaaatgg gatgactaat gaacggagat aatccctcac
    24781 ctaaccggcc ccttgttaca gttgtgtaca aggggcctga tttttatgac ggcaaaaaaa
    24841 aaccgccagt aaaccggcgg tgaatgcttg catggataga tttgtgtttt gcttttacgc
    24901 taacaggcat tttcctgcac tgataacgga tcgttgacac agtagcatca gttttctcaa
    24961 tgaatgttaa acggagctta aactcggtta atcacatttt gttcgtcaat aaacatacag
    25021 cgatttcttc cggtttgctt accctcatac attgcccggt ccgctcttcc aatgaccaca
    25081 tccagaggtt cttcaggaaa tgcgcgactc acacctgcag tcacggtaat gttgatatgc
    25141 ccttcagaat gtgtgatggc atggttatcg actaactggc aaattctgat acctgcacga
    25201 catgcttctt catcattagc cgctttgaca ataatgataa attcttcgcc accgtagcga
    25261 taaaccgttt cgtaatcgcg cgtccaactg gctaagtaag ttgccagggt gcgtaatact
    25321 acatcgccga ttaaatgccc gtaggtatca ttaaccaatt taaatcggtc aatatccaac
    25381 aacattaaat aaagattcag aggctcagcg ttgcgtaact gatgatcaaa ggattcgtca
    25441 agaacccgac gacccggcaa tcccgtcaaa acatccatat tgctacggat cgtcagcaaa
    25501 taaattttgt aatcggttaa tgccgcagta aaagaaagca acccttcctg aaaggcgtcg
    25561 aaatgcgcgt cctgccagtg attttcaaca atagccaaca ttaattcccg accacagtta
    25621 tgcatatgtt gatgggcaga atccattagc cgaacgtaag gtaattcatc gttatcgagt
    25681 ggtcccagat gatcaatcca ccgaccaaac tggcacagtc cataagaatg gttatccgtt
    25741 atttctggct tactggcatc tctcgcgacc acgctgtgaa acatactcac cagccactgg
    25801 tagtgggcat cgatagcctt attgagattt aacaagatgg catcaatttc cgttgtcttc
    25861 ttgatcattg ccactccttt ttcacagttc cttgtgcgcg ctattctaac gagagaaaag
    25921 caaaattacg tcaatatttt catagaaatc cgaagttatg agtcatctct gagataacat
    25981 tgtgatttga aacaaaatca gcagataaaa aagtgtttaa ttctgtaaat taagcctgca
    26041 ttatcgcaat taaaaggatg acaaatagca taaaccaata ccctaatggc ccagtagttc
    26101 aggccatcag gctcatttat ttttatttct gcaaatgagt gacccgaacg acggccggcg
    26161 cgcttttctt atccagactg ccgctaatgt tgatcatctg gtccggctga acttctcgtc
    26221 catcaaagac tgccgcagga ataacaacat taatttcacc gctcttatcg cgaaaaacgt
    26281 aacggtcctc tcctttgtga gaaatcaaat taccgcgtag tgaaaccgaa gcgccatcat
    26341 gcatggtttt tgcgaaatct acggtcattt tttttgcatc atcggttccg cgatagccat
    26401 cttctattgc atgaggcggc ggtggcgctg catcctgttt taaaccgccc tggtcatctg
    26461 ccaacgcata aggcatgaca agaaaacttg ctaatacaat ggcctgaaat ttcatactga
    26521 ctccttaatt gcgtttggtt tgacttatta agtctggttg ctattt
//
LOCUS       DAYPHN010000060        26150 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000060 DAYPHN010000000
VERSION     DAYPHN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.060"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..485
                     /locus_tag="ACG0BW_002326"
     CDS             <1..485
                     /locus_tag="ACG0BW_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFK7259278.1"
                     /translation="ADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVLHARRTTGLQ
                     LPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRATAIQHFLSAR
                     LRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATVQVWCLCRTL
                     "
     gene            544..1119
                     /gene="nfuA"
                     /locus_tag="ACG0BW_002327"
     CDS             544..1119
                     /gene="nfuA"
                     /locus_tag="ACG0BW_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFK7259279.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            1480..2796
                     /gene="gntT"
                     /locus_tag="ACG0BW_002328"
     CDS             1480..2796
                     /gene="gntT"
                     /locus_tag="ACG0BW_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFK7259280.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(2844..4925)
                     /gene="malQ"
                     /locus_tag="ACG0BW_002329"
     CDS             complement(2844..4925)
                     /gene="malQ"
                     /locus_tag="ACG0BW_002329"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFK7259281.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVVKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVILRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKK"
     gene            complement(4935..7328)
                     /gene="malP"
                     /locus_tag="ACG0BW_002330"
     CDS             complement(4935..7328)
                     /gene="malP"
                     /locus_tag="ACG0BW_002330"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFK7259282.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            7951..10656
                     /gene="malT"
                     /locus_tag="ACG0BW_002331"
     CDS             7951..10656
                     /gene="malT"
                     /locus_tag="ACG0BW_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFK7259283.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(10699..11715)
                     /gene="rtcA"
                     /locus_tag="ACG0BW_002332"
     CDS             complement(10699..11715)
                     /gene="rtcA"
                     /locus_tag="ACG0BW_002332"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFK7259284.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITDIRAGRAK
                     PGLLRQHLTAVKAATEICRATVEGAELRAQRLIFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRISAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(11719..12945)
                     /gene="rtcB"
                     /locus_tag="ACG0BW_002333"
     CDS             complement(11719..12945)
                     /gene="rtcB"
                     /locus_tag="ACG0BW_002333"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFK7259285.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            13134..14732
                     /gene="rtcR"
                     /locus_tag="ACG0BW_002334"
     CDS             13134..14732
                     /gene="rtcR"
                     /locus_tag="ACG0BW_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFK7259286.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHAALTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFADSGRITLETVEDEINRLRYNWQES
                     RPSALPALLGAEAENIDLFDRLQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(14714..15472)
                     /locus_tag="ACG0BW_002335"
     CDS             complement(14714..15472)
                     /locus_tag="ACG0BW_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFK7259287.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(15489..16319)
                     /gene="glpG"
                     /locus_tag="ACG0BW_002336"
     CDS             complement(15489..16319)
                     /gene="glpG"
                     /locus_tag="ACG0BW_002336"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFK7259288.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(16364..16690)
                     /gene="glpE"
                     /locus_tag="ACG0BW_002337"
     CDS             complement(16364..16690)
                     /gene="glpE"
                     /locus_tag="ACG0BW_002337"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFK7259289.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            16880..18385
                     /gene="glpD"
                     /locus_tag="ACG0BW_002338"
     CDS             16880..18385
                     /gene="glpD"
                     /locus_tag="ACG0BW_002338"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFK7259290.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNVHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(18554..18753)
                     /locus_tag="ACG0BW_002339"
                     /pseudo
     CDS             complement(18554..18753)
                     /locus_tag="ACG0BW_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981057.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18892..20022)
                     /locus_tag="ACG0BW_002340"
     CDS             complement(18892..20022)
                     /locus_tag="ACG0BW_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259291.1"
                     /translation="MLTYFWGNIKAICILFVLLVSSCYNIVYAEDMGRDRAFCYPGAP
                     GNNTTPATFSYNFGTVIVSDVNNNSPGTVLPSQDWKIGHYKAYCNSLDDYEVYFSGVS
                     GIDPSGASGDHQGSDIFIPLTHEISVSTHVKLYNKNGTMTDKAVPFDNYSTNYPADRS
                     KPSSWASGTEGYIKIRLDKKIISDISINNVLLVSLYVSQFPTEHGPIPVFNAYIGNLN
                     IEVPQGCTINEGTSFTVNMPDVWASELSRAGAGVKPTGVSPVATTIPINCTNKDIDAV
                     MTLVFDGNISSTRDVNGRQSIIQAEDNPDVGIMIMDSQQESVDLNALAAHVGVPFKLV
                     ENRAESAQTADVTFLSLPVSTTGRRPAAGRYNALAVLRVEYQ"
     gene            complement(20036..20815)
                     /locus_tag="ACG0BW_002341"
     CDS             complement(20036..20815)
                     /locus_tag="ACG0BW_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFK7259292.1"
                     /translation="MHQFPLSSLMINVLICILICCTNAQAGVVVGGTRFVYPEKQSSI
                     SVELKNTASKDMLVKISVTPDDGRQLKGTLESQPLLPDKAFIATPPLLVLKQDKNTKI
                     RINRVGGQLPIDRESLFILNIAALPAQEQNSQLKNENQLQVAVRNRMKIFYRPETLSG
                     NPLDAYQQLRWSRNNEAVTVFNPTPWYVTLYNLSIDGKSINGGMVAPFSHRQQDWCQR
                     QGYCEINWQTLDLYNNALPVWSVKLNVLQNNAIMGAVAIHG"
     gene            complement(20781..21305)
                     /locus_tag="ACG0BW_002342"
     CDS             complement(20781..21305)
                     /locus_tag="ACG0BW_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259293.1"
                     /translation="MTRYWNLIALLFSVISMSSIADDTTHTGVVRIEGIITAKTCIVA
                     DESKNFTVNMPDVPSYMVRNSGSVTEKIGFSVKLTRCGSDVSHANVTFDGIPVSEDAA
                     LYKLEEGSVDGLALAIFDNNRQSISNGIKSTSYALTPLVDNTLHFFAAYKAIKNDVQP
                     GDANASVSFIVTYD"
     gene            complement(21313..21876)
                     /locus_tag="ACG0BW_002343"
     CDS             complement(21313..21876)
                     /locus_tag="ACG0BW_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259294.1"
                     /translation="MNILQKFILLMFRSLFMMVISYNALANDTVVVTFTGQLLAKTCD
                     ITTGSKDQPVNMGTYDVNDFLNVGDVSGSHTFTINLEGCPTTTSTVYSSGVSANVQFT
                     GDADAINTTLLRLTSSADSATGVGIEILDSNDKIIAINSESGFKELILNNNGDASLTF
                     KLRYKSTQQNVHAGQANALLYFDIDYQ"
     gene            complement(21876..24467)
                     /locus_tag="ACG0BW_002344"
     CDS             complement(21876..24467)
                     /locus_tag="ACG0BW_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFK7259295.1"
                     /translation="MNLKLRKLEHRMARQKQMRWCMLLLLTLLPACSVAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEIWVNDSPLTSRKVTFKADEQNQLIPCFSLDE
                     LVTLGINKSALPEQAQAEGNNCLDLRIWFAEVQYTPELDAQRLKLTFPQAILKHEARG
                     YIPPEEWDNGIPAILINYDFSGNSDRGDYASDSYYLNLRSGLNLGAWRFRDYSTWSRS
                     GNSPGKIAHVSSTLQRVIIPFKSELTLGDTWSSSDVFDSVSIRGAKLESDENMLPDSQ
                     SGFAPTVRGIAKSNAQVTIKQNGYVIYQTYTPPGPFEINDLNPTSSAGDLEVTIKESD
                     NSETIYTIPYAAVPILQREGHIKYSATAGKYRSNSYNQNSPNVFQGDLIWGLPWDITA
                     YGGMQYSNNYRAIALGLGFNLGMFGATSFDLTQANSTLADGSKHQGQSYRFLYSKSLV
                     QTGTAFQIIGYRYSTEGFYSLSDTTYKQMSGTVIDPDTLNEKDYVYNWNDFYNLRYNK
                     RGKFQANISQPLGEYGSMYLAASQQTYWNTNNKDTLYQVGYNTSVHGIYLNIAYNYSK
                     SPGSDADRILSVNVSLPISNWLSLAVDGRTATNSMTATYGYSQDNSGHVNQYAGVSGS
                     LMEQHNLNYNIQQNFANQNNGENGSVGINYRGTYGSLNSGYSYDNNGNQQFNYGLSGA
                     LVIHENGLTLSQPLGETNILIKAPGANNVNVERGTGVKTDWRGYAVVPYATEYRRNNI
                     SLDPMSMSMHTELDATTMEVIPSKGALVRAEFNAHVGIRGLFNVHYRNKPVPFGATVS
                     VPTEGTTQITGIVGDSGQVYLSGLPLQGIINIQWGESSYQQCRANYQLPESELDNAIS
                     YANLECR"
     gene            complement(24509..25261)
                     /locus_tag="ACG0BW_002345"
     CDS             complement(24509..25261)
                     /locus_tag="ACG0BW_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFK7259296.1"
                     /translation="MRWISKNKCFSNRNINSLLKTQYIIRAGALFLLSAISINAIAAG
                     GFGLGSTRLVYPSDSQQITLSVQNGGANTSYLIQSWIDDNQSSKKSQDFVVTPPLFML
                     PTRKEASLRIMFIGKSQLPTDRETLFWMNVKAIPPTDEKNTQKNTLQLALQNRIKLFY
                     RPTNLPVQAGKARDMLRFKYEGKQLRVINPSPYYLTVTGIRVQGAKLPNAFVPPKSDV
                     TVSSPVTLAGEITYQTINDYGATTARQKGTMQ"
     gene            complement(25382..26086)
                     /locus_tag="ACG0BW_002346"
     CDS             complement(25382..26086)
                     /locus_tag="ACG0BW_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7259297.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDASQFGGADTDWSTVD
                     YPKLTDMDSNTDAMGGNIRFTGRVVKATCKVATESKQIEVVLPVVPSNAFTGIDTEAT
                     GASNQTDFNIKLTDCSNTDSQNIEFRFTGVADGANQTLANEVEGAADADGTGEAGATG
                     VGIRIYSKNTTTDGLIKLNTQTPQGSSSSAAYVIPGDAATHDFTAAFTAGYAQNGSTV
                     APGLVKSTASFVVLYE"
BASE COUNT         6500 a         6811 c         6141 g         6698 t
ORIGIN      
        1 ttgccgacta tgcgccgcca ttaagtccgc ttatccacca gcttaagttt tcccggcgca
       61 gcgaaatcgc cagcgccctg gcacgtctgt tactattgga agtcttacac gcccgtcgca
      121 ccaccggctt gcaactgcct gatcgcatca tcagcgttcc gttatggcag cggcgtcact
      181 ggcgtcgggg atttaatcag agcgatttgc tgtgtcagcc gttatcacgc tggttgcact
      241 gccaatggga tagcgaagcc gtcacacgaa cacgggccac cgctatccag cattttctca
      301 gtgcccggct gcgcaagcgc aacctgaaaa atgcctttcg ccttgaattg cctgtgcaag
      361 gtcgccatat ggtgattgtg gatgatgtcg tcaccaccgg aagtaccgtc gcagagattg
      421 cgcagttgct tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt tgtcgaacct
      481 tgtagagcct cgatgatggg cgtattataa ccaactaaaa tagtcaacta ttaggccatt
      541 actatgatcc gtatttccga tgctgcacaa gcgcactttg ccaaactgct ggcaaatcag
      601 gaagaaggga cacaaatccg cgtatttgtg attaaccctg gcacgcctaa cgctgaatgt
      661 ggcgtttctt attgtccgcc ggacgctgta gaagccaccg acactgccct gaaatttgac
      721 ctgctgaccg cgtatgttga tgagttaagc gctccttacc tggaagatgc agagatcgat
      781 tttgttaccg accagttggg ttctcagcta acactgaaag cgcctaacgc caaaatgcgt
      841 aaagtcgcag acgatgcacc gctgatggag cgcgttgagt atatgctgca gtcgcagatc
      901 aacccgcaac tggctggtca cggtggtcgc gtttcgctga tggaaatcac cgaagacggt
      961 tacgctattc tgcaatttgg cggcggctgt aacggttgtt ccatggtcga tgtgacgctg
     1021 aaagaaggga tcgagaagca gttgctgaac gaattcccgg agctgaaagg tgtgcgcgat
     1081 ctcaccgaac accagcgcgg cgagcactcc tactactaag ttatcccctc ttttgtagat
     1141 tgcccgatgc gacgctaaag cgtcatttcg ggcctacgtt gaacatgagc cagacgttgg
     1201 cgcagcattg ctttggcaaa tccctccctt cgtttccccg cgataatatg accaacctct
     1261 cataatttaa atttaccccg ctctggtgat tctcaaacgc cggatgttac ccgtatcatt
     1321 cacgtgggta ccaaacatac tcctgacatc tgactacaat aattagtttt agtgggtatc
     1381 agtcgtggtg ccgcaatatc tctgttcccg attgggataa ttagagtttg tcgtcagaaa
     1441 attgacgtta cccataacaa atgaaaggcc aggtaaatca tgccattagt cattgttgct
     1501 atcggtgtaa tcttgttgtt gctcctgatg atccgtttca aaatgaacgg cttcatcgct
     1561 ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa tgccgctgga taaagttatt
     1621 ggctccatca aagccggtgt cggcggaacg ctcggtagcc ttgccctgat catgggtttt
     1681 ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg cacaacgtat cgccaccacg
     1741 ctgattgcta aatttggtaa aaaacacatc cagtgggcgg tggtactgac tggttttacc
     1801 gttggttttg ccctgttcta tgaagtgggc tttgtgctga tgctgccgct ggtgtttacc
     1861 atcgcggctt ccgcgaatat tccactgctg tatgttggtg taccaatggc ggctgcactg
     1921 tctgtgaccc acggcttcct gccaccgcat ccgggtccga ctgcgattgc caccattttc
     1981 aatgccgata tgggtaaaac cctgctgtac ggtactattc tggcaatccc gaccgtgatt
     2041 ctcgccggtc cggtttacgc tcgcgtgctg aaaggtatcg ataagccgat cccggaaggt
     2101 ctgtacagcg cgaaaacctt cagcgaagaa gagatgccga gctttggcgt cagcgtctgg
     2161 acctctctgg tgccggtagt gctgatggcg atgcgtgcga ttgccgaaat gatcctgccg
     2221 aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg acccagtaat ggctacgctg
     2281 attgccgtac tgattgcgat gttcaccttt ggtctgaacc gtggtcgttc aatggatcag
     2341 attaacgaca cgctggtttc ttccatcaaa atcattgcga tgatgctgtt gattatcggt
     2401 ggtggcggtg cgttcaagca ggtgctggta gacagcggcg tggacaaata cattgcttcc
     2461 atgatgcacg aaaccaacat ttctccgctg ctgatggcct ggtcgattgc tgccgtactg
     2521 cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg cgggtggtat cgcggcaccg
     2581 ctgattgcaa cgacgggtgt tagcccggag ctgatggtta ttgcggtggg ttccggtagt
     2641 gtgattttct ctcacgtgaa cgatccgggc ttctggctgt tcaaagagta ctttaacctg
     2701 actatcggcg agaccatcaa atcctggtcg atgctggaaa ccattatctc ggtgtgcggt
     2761 ctagtaggct gtctgctgct gaatatggtg atttgataac actgactgcc ggatgcatcg
     2821 catccggcat caaccgcacc ctactacttc ttcgctgccg ctctgcgccg tctgtccaaa
     2881 tccttcagca acttgttcac gccatcatcg gcaaacatcg cctcaagcgt tgcggagagc
     2941 ttgcgccgcc agtttttata ctggtaactg gtgccgggaa tattcaccgg ttcagccata
     3001 tccagccagt cttccggctg tagtcctaac agggcgctgt tgctatcggc gatgtagcgc
     3061 tgtaagccac gattcagcgt cggcgtcatc gacatcaacg atgccttatg cccggcacgt
     3121 tttggcagac aaccatattt atgcagagca tccagtagtc cttgcttcgc cagttcgcga
     3181 tcctgataca gaccgcgcag tatcacttca tccggataca accccagcgt tttgcccagc
     3241 gttagatccc cgctctccca gtagccgcgt agcgtaggca ggtcgtgtgt cgccgcaacc
     3301 gccatcgact gctctgggta cgctttcggt gagcggaatg tcttctcgtg gtcgttttcg
     3361 aaatagagca ctttgtagga gtacacaccg ctgctgcgca gcttaccgac aatctctacc
     3421 ggtacggtac cgagatcttc accaatcacc atacagcgat gacgcttact ttcgagtgcc
     3481 agaatcgaga gcagatcatc caccggatag tgaacatatg ccccctgatc tgccgtctcg
     3541 ccatacggta tccaccacaa acgcagcatc gacatcacat ggtcaattcg taatgcgccg
     3601 cagttttgca tattggcacg caacagctcg ataaacggtt cataggcacg cgcggtgata
     3661 atatgcgggt ccattggcgg caatccccag ttctgcccca acgggccgag gatatccggc
     3721 ggcgcgccaa ccgacgcttt caggcaatac aactcccggt cgcaccaggt ttctgctcca
     3781 ccctctgcta caccaacggc caggtcacga tacaagccaa tcggcatttc atagccctgg
     3841 cttatctccc agcaggcggc aaactggctg taagccagcc actgcaacca gagataaaaa
     3901 tcaacgtcat cgcgatgttc ttcgcagaac tggcgcactt ctggtgaatc cacgttctga
     3961 tacatctctg gccatgcagg ccagccccag cgcatttcgt cctctttcac ttgctgggca
     4021 tgtagtgcgt caaacgccgc ctgccagaac aggctgtcgc cttgctctgc aacaaactgg
     4081 cgaaacgcgg ccatctgttc atcatcacgt tgcgcgaaac ctttccacgc cattcgtaat
     4141 gctgtcattt ttagggcggt aaccgtggag taatcgaccc agtcggcatc ccgcgcctgt
     4201 tgcagcgtct gttgcgtggt cggcaacttc caccaggcct gagcctcttc gctaagatgg
     4261 aaatcttcaa cggcgttaac gtcgatataa atcacattca gccaacggcg agaagacggg
     4321 ctatacgggc tggcactttc cggatttgcc ggatagagcg catgaatcgg gttcagaccg
     4381 atgaacgacc cgccacgttt taccacatcc accagcatcg ctttgagatc gccaaaatcc
     4441 ccaatacccc agtttttttc cgatcgcagc gtataaagct gaacgcaggc accccacagc
     4501 ttttgtttgt tcagcaacgc ctgcggttcg taacagcgtt tcggggcgac aatcacccgg
     4561 caatgcgcgc gctggtcgtc ctgggtgagt gtcagcgtgt gataaccttc cggcagcttc
     4621 gtcggtagat tgaacgcttt gccccccgtt acatggcctt tgtactgcgt tccttcttcg
     4681 gtggtcagca gccagctata ttcgccgctg ccctccacca ccatcggcat ttttttgccg
     4741 ctggtataaa ccatgacatt cgggactggc gttaccgcca ctttcgtggc ggtacgttga
     4801 tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg tttaccgtgg
     4861 gcattgatgt aattggggct aatccccgcc gccagcgcgg cattatccag acgtttgctt
     4921 tccatcgagc ttccttagcg ttttgcctgc cagatacgag cctgataatc gcgaatagag
     4981 cgatccgagc taaacatacc gcagcgggcg gtattgagga tcgccgcgcg agtccaggcc
     5041 tcctggtcgc ggtacagcac atccacctgc ttttgtgcct ctacataggc tgcgaaatcc
     5101 gccatcacca gatacggatc gccgccctgt ttgccgatac tgtgcagcat ctggtcgaag
     5161 gcatgcttat cgccgtcgct gtatttgccg ctttccagct ctttcaatac tgcgtccagc
     5221 accttatctt tcttccgcca tttcaccggg tcgtagcctt tggccagaat tgccttcact
     5281 tgttccacgg tatgaccaaa aataaagata ttttcttcac caactttctc ggcgatttca
     5341 acgttcgcac catccagcgt accgacagta agcgcaccat tgagcgccag tttcatattg
     5401 ccggtaccgg aagcttcttt acctgccgtc gaaatttgtt cggagatatc cgccgccggg
     5461 atcagttttt ccgccgccga aacgcaataa tccggcagga acaccacctt caacttgtcg
     5521 ccaaccagcg gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa gataatattc
     5581 ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga ggaagacgcg cggtacgcga
     5641 tcagcctgcg ggttttcacg aatttctttg tacaacgcca gaatatgcag cagattcagg
     5701 tgctggcgtt tgtactcatg cagacgtttg atctgaatat cgaaaatcgc ctgtggattg
     5761 atctcaatac cggtacgaac tttcacaaac tctgccagac ggactttatt cgcctgcttg
     5821 atctcgcgat attgctgacg gaatttcgca tcatcagcga atttttccag attgatcagt
     5881 tgatcgagat cgttagccca ctcttttttc agtgatttat ccaacagagc cgccagtgcc
     5941 gggttgcact gtttgatcca gcgacgtggg gtaataccgt tggtgacgtt atggaatttg
     6001 ttcggccata gctggtgata ttccgggaac agatctttca ccaccagatc cgagtgcagg
     6061 gccgcaacac cgtttaccgc gaaaccgcca accacacaca ggttcgccat atgcacttgt
     6121 ttgtcgtgta ccaccgccag tttggcccat actttttcat cgcccggcca ggttttttcc
     6181 accagcgttt taaagcgtgt attaatttcg ttaataatct gcatgtggcg cggcagtaag
     6241 cctttcacca gtttcacatc ccagcgttcc agcgcttctg gcatcaaggt atggttggtg
     6301 taagcgaaag ttttactggt gatggcccag gcatcatccc agctcatctg gtgctcatcg
     6361 atcagcacgc gcagcagttc tggaatcgcg atagttgggt gggtatcgtt cagctgaata
     6421 acttcgtact ccgccagctc gtgtagttta cgccccgcca gatgatggcg acgcaaaata
     6481 tccgctaccg agcaggcaca ctggaagtat tgctgcatca ggcgcagctt tttaccggca
     6541 gtatggttgt cgtttggata gagaactttg gtcagttttt ccgcgttgat gccctgctgt
     6601 tcggcacgca ggaaatcacc atcgttaaat ttagtcagat caaacggatg cgcatgcgtc
     6661 gcctgccaca gacgcagtgg ctgcgccacg ccattacgat agccgacaac ggggagatcc
     6721 cacgcttgac cagtaatggt aaattccggc tcccagcgtc cgtctttcgt cactttaccg
     6781 ccaattccaa cctgcacatc cagtgcttca ttgtggcgga accacggata gttaccacga
     6841 tgccagtcat ccggcgcttc aacctgtttg ccatcgacaa atgactggcg gaacaagcca
     6901 tattgataat taaggccgta accagtagct gactgcccga cggtcgccat tgagtcgagg
     6961 aagcatgccg ccagacgtcc cagaccaccg ttacccagcg ccgggtcgat ctcttcttcc
     7021 agcaagtcag tcaggttgat gtcataagcc ttcaacgaat cctgtacatc ctgataccag
     7081 ccgagattca acaggttgtt gcccgtcagg cgaccaatca aaaactccat tgagatgtag
     7141 ttaacatgtc gctgattcgc caccggtttg gcaaatggct gagcacgcag catttcggcc
     7201 agtgcttcgc tcactgccag ccaccactga cgaggggtca tttcagccgc agaatttaag
     7261 ccataacgct gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt aaaaataggt
     7321 tgtgacatag gagttccact tttcttagat tttcaacaca acgttatcgc tagtttgcca
     7381 ggctcgatgt tgaccttcct catcctgcgg gggattaggc agggaggagt tgcggggatg
     7441 agcaaggaaa tgtgatctcg accacttaaa gctagtgcaa accacaggat tagcagcaaa
     7501 tcaatgccat acagcgcaga aaatctgtat ctaagtgcaa aaaatggccg ttgcgtattt
     7561 tcaaaaagcg gaaggtaact ctataaatta agtaaaggag tgaaacagtt tcataagtaa
     7621 aatatcaagt gtgctccatc tcattcttaa tagatttatt aagatcatct ttttagatgg
     7681 cactttcatc agggataaag aaaaaaccct tgcttaaatg aaactgatga acataaggga
     7741 aataccgtac aacccagcat tcacgctgta tcagcgtcgt tttaggtgag tcattaataa
     7801 agatttggaa ttgtgacaga gtgcaaattc agacacataa aaaaacgtca tcgcttgcat
     7861 tagaaaggtt tctggccgac cttataacca ttaattacga agcgcaaaaa aaataatatt
     7921 tcctcatttt ccacagtgaa gtgattaact atgctgattc cgtcaaaatt aagtcgtccg
     7981 gttcgactcg accataccgt ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac
     8041 aacttccggc tggcgctgat cacaagtcct gcgggctacg gaaagaccac gctcatttcc
     8101 cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc tggatgaagg tgataaccag
     8161 caagagcgtt tcgccagcta tctcattgcc gccgtgcagc aggcaaccaa cggtcactgt
     8221 gcgatatgtg agacgatggc gcaaaaacgg caatatgcca gtctgacgtc actcttcgcc
     8281 cagcttttca ttgagctggc ggaatggcat agcccacttt atctggtcat cgatgactat
     8341 catctgatca ctaatccagt gatccacgag tcaatgcgct tctttattcg ccatcaacca
     8401 gaaaatctca ccctggtggt gttgtcacgc aaccttccgc aactgggcat tgccaatctg
     8461 cgtgttcgtg atcaactgct ggaaattggc agtcagcaac tggcatttac ccatcaggaa
     8521 gcgaagcagt tttttgattg ccgtctgtca tcgccgattg aagccgcaga aagcagtcgg
     8581 atttgtgatg acgtttccgg ttgggcgacg gcactgcagc taatcgccct ctccgcccgg
     8641 cagaataccc actcagccca taagtcggca cgccgcctgg cgggaatcaa tgccagccat
     8701 ctttcggatt atctggtcga tgaggttttg gataacgtcg atctcgcaac gcgccatttt
     8761 ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcac tcatcacccg tgtgaccggc
     8821 gaagaaaacg ggcaaatgcg cctcgaagag attgagcgtc aggggctgtt tttacagcgg
     8881 atggatgata ccggcgagtg gttctgctat cacccgctgt ttggtaactt cctgcgccag
     8941 cgctgccagt gggaactggc ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc
     9001 tggatggccc agggatttcc cagcgaagcg attcatcatg cgttggcggc aggcgatgcg
     9061 ctgatgctgc gcgatattct gcttaatcac gcctggagtc tgttcaacca tagcgaactg
     9121 tcgctgctgg aagagtcgct taaggccctg ccgtgggaca gtttgctgga aaatccgcag
     9181 ttagtgttat tgcaggcgtg gctgatgcaa agccaacatc gctacggcga agttaacacc
     9241 ctgctagccc gtgctgaaca tgaaatcaag gacatcagag aaggcaccat gcacgcagaa
     9301 tttaacgctc tgcgcgccca ggtagcgatt aacgatggta atccggatga agcggaacgg
     9361 ctggcaaaac tggcactgga agaactgcct ccgggctggt tctatagccg cattgtagca
     9421 acctcagtgc tgggtgaagt attgcactgc aaaggcgaat tgacccgctc actggcgtta
     9481 atgcagcaaa ccgaacagat ggcgcgccag cacgatgtct ggcactacgc cttatggagt
     9541 ttaatccagc aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc gtgggaaacg
     9601 caggaaaaag cgttccagct gatcaacgag cagcatctgg aacagctgcc aatgcatgag
     9661 tttctggtgc gcattcgtgc gcagctgtta tgggcctggg cgcggctgga tgaagccgaa
     9721 gcgtcggcgc gtagcgggat tgaagtcttg tcgtcttatc agccacagca acagcttcag
     9781 tgcctggcaa tgttgattca atgctcgctg gcccgtggtg atttagataa cgcccgtagc
     9841 cagctgaacc gtctggaaaa cctgctggga aatggcaaat atcacagcga ctggatctct
     9901 aacgccaaca aagtccgggt gatttactgg caaatgaccg gcgataaagc cgccgctgcc
     9961 aactggttgc gtcatacggc taaaccagag tttgcgaaca accacttcct gcaaggtcaa
    10021 tggcgcaaca ttgcccgtgc acaaatcttg ctgggcgagt ttgaaccggc agaaattgtt
    10081 ctcgaagaac tcaatgaaaa cgcccgcagt ctgcggttga tgagcgatct caaccgtaac
    10141 ctgttgctgc ttaatcaact gtactggcag gccggacgta aaagtgacgc ccagcgtgtg
    10201 ttgctggacg cattaaaact ggcgaatcgc accggattta tcagtcattt tgtcatcgaa
    10261 ggcgaagcga tggcgcaaca actgcgccag ctgattcaac tcaatacgct gccggaactg
    10321 gaacagcatc gtgcgcagcg tattctgcga gaaatcaatc aacatcatcg gcataaattc
    10381 gcccatttcg atgagaattt cgttgaacgt ctgctaaatc atccggaagt gccagaactg
    10441 atccgcacca gcccgctgac ccaacgtgaa tggcaggtcc tggggctgat ttactccggt
    10501 tacagcaacg aacaaattgc aggcgaactg gaagtggcgg caaccaccat caagacgcat
    10561 atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc aggctgccgt gcagcacgcg
    10621 caaaaactgc tgaagatgat gggatatgga gtgtgagttt agccggataa tacgtcggat
    10681 ccggcttaca tctttatatc attcaatgct cacccgcgtt acgccatccg cctccaccag
    10741 actaaaccgc actggcaaga aacgctccac caccgcaata ttggtcagca gatggcatga
    10801 gggatgggcg accgtaaatt ctcccgcgcc cgccagcgcc atcggcagta ccagctggtc
    10861 ggcgagatat tcccccaccg ccgccgggct tgccaggtag cgtttcacct ctttcaccaa
    10921 ctgtgccgcg accacttcgg cactgatgcg cttttcaccg acgacaaaaa agcgttcggt
    10981 gatattttca ctttcgactt caagcgagac ggtattaccc ggcccctggt cacgcggcag
    11041 gttatgaata ttctgctcat gcagggaaaa actacccgcc agcgtagcga tttcacgctc
    11101 agcaacatgc ctcggcacgc cagctaatag cacctctcca cgcatctgca caatgttccc
    11161 gcgctcgcca agttgcaagg tgttaaacaa tgccacgggc gagacttccg ttgccaccac
    11221 cccgcctcca gcaggataaa aaccgtggcg taataatgtg gtttgctgat gaatgcccat
    11281 tttcgccagc agcggctcca gcacccggcg gataaaatcc gcaggcggtg ccgacgggtt
    11341 atcggtgccg ccgctcactt caacacgcga aggcccatca gcaaaccaca gcgcgggtag
    11401 caccgtttgc agcaccagcg tgctgcttcc ggcgcttccg atagcaaagc ggtaatcgcc
    11461 gccgcgcacg gtgccgggcc ggaagatcag acgctgcgcc ctcagctccg cgccttccac
    11521 cgttgctcgg caaatttccg tcgccgcctt aaccgcggtc agatgctggc gcaacagccc
    11581 cggtttcgcc cgcccggcac gaatgtcagt gatggtaaat ggctggccag ttatcatcga
    11641 caggctcagc gccgagcgca ggatctgccc gccgccttcg ccctgtgcgc catccagcgc
    11701 aatcatcctt ttcatcattt atccttttac gcacaccacc tgacgcaggg tatagataac
    11761 ttccaccaga tcgctttgtg ccgccatcac cgcatcaata tctttatacg ccatcgggat
    11821 ttcgtcgatc acttcggcat ctttacggca ttccacatgc gcggtggcac gaatttgatc
    11881 ttccacgctg aacatttttt tcgctttagt acggctcata acccgcccgg caccgtggct
    11941 gcacgaacag aacgactctt catttcccag cccacggacg ataaagctct tcgcccccat
    12001 cgaaccggga ataattccat attgaccagc acgcgcagac accgcgcctt tacgcgtcac
    12061 gtagatctct tcaccaaagt gctgttcttt ttgcacatag ttgtggtgac agttgatctc
    12121 ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc gtaatgctct gcaacgccgt
    12181 taccacgttt tccatcatcg catcgcggtt aaggctggca aaaagctgcg cccaggccac
    12241 ggctttcagg taatcatcaa agtattccgt accttccata aagtacgcca gatcacgcga
    12301 cggcaatgtt tcaagcgttt cctgcatctc tttttgcgcc agatcgataa agtaagttcc
    12361 gatggcatta ccgattccgc gtgaaccgga gtgcagcata atccacacct gatccgactc
    12421 atcaaggcag atttcaataa agtggttacc cgttcccagc gttcccaggt gtttatagtt
    12481 gttagtgtta aggaaacggg gatatttttg cgttaaccac tgataaccgg cttcaagctc
    12541 agcccatttc gcatcgacgt taacaggcgg gttttcccat gcgcctttat cacgtttaca
    12601 acgcccggta gtacgcccgt gcggcacggc agtttcaatt gcctgacgca gctccgccag
    12661 gttttccggc aggtctgccg ccgttaacgc ggtacgcagc gcgttcattc cacaaccgat
    12721 atccacgccc accgccgccg gaataatcgc ccctttggtt gggatcacgc taccaatggt
    12781 ggaaccttta cccaggtgta catcaggcat taccgcaata tgtttgaaaa taaacggcat
    12841 cttcgccgta ttaataagtt gctgacgcgc atcggcctct accggcacgc ctttggtcca
    12901 catttttacc ggggcatttt cagtggtcag taattcgtaa ttcattttgt ttttctcttt
    12961 tcgttgtttg ctgtcctgat aattgcaacc gtcgtgccag aaaattaaat aagcagctta
    13021 attttttaat ttattgtttt taatagatta aatctttacg tccgtaaacg gagatttccc
    13081 gcaaagccaa tttaccgata atgaaatatc gtcttttata aggatatcta gagatgcgta
    13141 aaacagtggc ttttggtttt gtcggaacgg tgctggatta tgccgggcgc ggcagtcagc
    13201 gctggtcgaa atggcgtccg acgctttgtt tatgccagca agaatcgttg gtcatcgatc
    13261 gactggaatt attgcatgac gcccgctcgc gctcgctatt tgaaacgctt aaacgcgata
    13321 tcgccagcgt ttcgccagaa acagaagtgg tgggcgttga gatagaactg cataacccgt
    13381 gggatttcga agaggtctac gcctgcctgc atgatttcgc ccgtggttac gcgttccagc
    13441 cagaaaaaga agactattta attcacatca ccaccggtac ccacgtcgcg cagatttgct
    13501 ggtttctgct ggcagaagcg cgttatctac ccgcccggtt gatacaatct tcaccaccgc
    13561 gcaaaaaaga acaaccgcgc ggcgcaggtg aagtgacgat tatcgatctc gatttaagcc
    13621 gttataacgc catcgccagc cgctttgccg aggaacgcca gcaaaccctc gatttcctca
    13681 aatccggcat tgccacgcgt aacccccact tcaaccgcat gattgagcag atcgaaaaag
    13741 tggcgattaa atcccgcgca ccgatcctgc tcaacggccc gaccggggcg ggtaagtcat
    13801 ttctggcgcg acgcatcttt gaattgaaac aggcgcggca tcagtttagc ggcgcgtttg
    13861 tggaagtgaa ctgcgccacc ctgcgtggcg ataccgccat gtcgacgctg tttggtcatg
    13921 tgaaaggcgc gtttaccggg gcgcgggagt cgcgggaagg attattacgc agcgccaacg
    13981 gcgggatgtt atttcttgat gagattggcg aactgggcgc agacgaacag gcgatgttgc
    14041 tgaaagccat tgaagagaaa accttttacc catttggcag cgatcgccag gtgagcagtg
    14101 attttcagct gatcgccgga acggtgcgcg atttgcgcca gctggttgcc gaaggcaaat
    14161 ttcgcgaaga cctgtacgca cgcattaatc tctggacctt caccctgccg gggctgcgcc
    14221 agcgccagga agatatcgaa ccgaacctgg attatgaagt ggagcgccac gccgcgctta
    14281 ctggcgacag cgtgcgtttt aacaccgaag cacggcgcgc ctggctggct tttgcgacat
    14341 ctccccaggc aacatggcgc ggtaactttc gagaactttc tgccagcgtc acgcggatgg
    14401 caacctttgc cgatagcggg cgcattactc tggaaaccgt agaagatgag ataaaccgtc
    14461 tgcgctataa ctggcaggag agtcgcccct ccgcgcttcc ggcgttgctg ggcgctgagg
    14521 cagaaaacat cgacctcttt gatcgactgc aactggaaca cgtaatagcc atctgccgcc
    14581 aggcaaagtc actttctgct gccggacgcc agctttttga cgtttcgcgc cagggcaaag
    14641 ccagcgtcaa tgacgcggat cggctacgca aatacctggc gcgttttggt ctgacgtggg
    14701 aagccgtgca ggatcagcac agctccagtt gaatatgatg gtccgtcagc acctgcatca
    14761 cgcttactgg cggcggggcg tcggtgtaga cggcatctac catgctgata ctgcccatat
    14821 tgaccatcgc gttacggcca aatttcgagt gatcgacaac cagcataacg tggcgcgagt
    14881 tctcaataat ggcgcgtttg gtgcgaactt cgtggtaatc gaactccagc agcgagccgt
    14941 cgctatcgat gccactaatc cccagaatgc cgaaatcaag gcggaactgg gagataaaat
    15001 cgagcgtcgc ttcgcctatg atcccaccat cgcggctacg taattcgcca ccggcgagaa
    15061 taatgcgaaa atcttctttt accatcaacg tgttagcgac gttgagattg ttggtgacaa
    15121 tgcgcaaatt gctgtgatta agcagcgcgt gcgccaccgc ttccggggtg gtgccgatat
    15181 cgataaacag cgtcgagccg ttggggattt gctccgccac tttgcgggca atacgctctt
    15241 tttcttcggt ctgggtggct ttgcgatcgt gccacggcgt gttaaccgaa ctggaaggta
    15301 gcgccgcgcc gccatgatgg cgcaggatca ggttttgctc cgccagctca ttgaggtcgc
    15361 gacgaatagt ctgcgggctg acggagaagt gctccaccag ttcctcggta ctgacataac
    15421 cctgctgttt aaccagttcg ataataccgt tgtgacgttg tgtttgtttc atttataaat
    15481 ccctggaatt attttcgttt tcgcgcattg agcgaatcaa caaaagccat cgctaaaccc
    15541 acggctaacc cggcgatgtg tgctccgttc gccatcgaca tcccaaacaa atcaaaccat
    15601 ccggcgacaa tccagatcag cgcaaagata attaacccac gttgcaggta aatgccactt
    15661 tgcggatcgc gttcgccacg tagccagacg tagcccatca gcgcatacac cacgccagaa
    15721 agcccgccaa accacggccc gctgaatttt tgctgcacat agccgcttaa cagggcgcta
    15781 atgagagtaa tgacaattag cttaccgcta ccgaggcgtt tttccaccgc accgccgaga
    15841 taccaccacc agagcaggtt aaagaggata tgcatcagcg agaagtgcat taacgcgtgg
    15901 gtgaagtaac gccagaactc aaatttcagt gtcggatcga atggccaggc cagccataac
    15961 atcacttcct gatcgccgag aatttgcatg gcaataaaca ccaccacgca ggcgatcatc
    16021 atcacccagg ttaccgggcc tgcgcgttca cgcaaggcag caaagaaagg ataacggcga
    16081 taatgcaggc cactgccggt atggcccgcc tgccagctgg ccgccagata acgcggatct
    16141 gccgggtttt cgagaaaacg cgccagctcc gcccgcacgc gctcggcctg ggactcatcc
    16201 gccagccaga catcgctttg gttatgttgt tgaatcgtga ggataacacc ctgcgtcgcc
    16261 atgtaatcaa caaacgcctg cgccacgcgg gggttagcaa aagaggtaat catcaacatc
    16321 gttgctgtcg cttattccac acaaaagggg acagtataaa gcgttacgcg ccgtacgcca
    16381 cctctgcggg aaactgacgc tgccaggctt caaagccgcc gtcaatgcta tagaccacat
    16441 cgtagccctg ttgcagcaga tactgcgccg cacctttgct gctattgccg tggtaacaca
    16501 tcaccatcac cggtgtgtca aagtcgttat cacgcataaa cgcgcccagt gtgtcgttgg
    16561 ttaaatggaa agcctgcacc gcatgcccca ttgcgaaact ctgtggatcg cgaatatcga
    16621 ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgtc ggcaacgtta atacattcga
    16681 actgatccat gcgtctctct ttctttacaa acaagtgggc aaatttaccg cacagtttac
    16741 gtcgaagcgg cagataaacg ccataatgtt atacatatca ctctaaaatg ttttttcaat
    16801 gttacctaaa gcgcgattct ttgctaatat gttcgataac gaacatttat gagctttaac
    16861 gaaagtgaat gagggcagca tggaaaccaa agatctgatt gtgataggtg gcggcatcaa
    16921 tggtgctggt atcgcggcag acgccgctgg acgcggttta tccgtgctga tgctggaggc
    16981 gcaggatctc gcttgcgcga cctcctccgc cagttcaaaa ctcattcacg gtggcctacg
    17041 ctaccttgag cactatgaat tccgcctggt cagcgaggcg ctggctgaac gtgaagtgct
    17101 gctgaaaatg gccccgcata tcgccttccc gatgcgtttt cgcctgccgc atcgtccgca
    17161 tctgcgcccg gcgtggatga ttcgcattgg tctgtttatg tacgatcatc tgggtaaacg
    17221 caccagcctg ccgggatcaa ccggtttgcg ttttggcgca aattcagtgt taaaaccgga
    17281 aattaagcgc ggattcgaat attccgactg ttgggtagac gacgcccgtc tggtactcgc
    17341 caacgcccag atggtggtgc gtaaaggcgg agaagtgctt actcgtactc gtgctacttc
    17401 tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa gatatcgata ccggcaaaaa
    17461 atatagctgg caagcgcgcg gcctggttaa cgccaccggc ccgtgggtaa agcagttctt
    17521 cgacgacggg atgcatctgc cttcgcctta tggcattcgc ctgatcaaag gcagccatat
    17581 tgtggtgcca cgcgtgcata cccagaagca agcctacatt ctgcaaaacg aagataaacg
    17641 tattgtgttc gtgatcccgt ggatggacga gttttccatc atcggcacca ccgatgtgga
    17701 atacaaaggc gatccgaaag cggtgaagat tgaagagagt gaaatcaatt acctgctgaa
    17761 agtgtataac gtgcacttta aaaaacagct tagccgtgac gatatcgtct ggacctattc
    17821 cggtgtgcgt ccgctgtgtg atgatgagtc cgactcgccg caggctatta cccgtgatta
    17881 cactcttgat atccatgatg aaaacggcaa agcaccgctg ctgtcggtat tcggcggtaa
    17941 gctgaccacc taccgcaaac tggctgagca cgctctggaa aaactgacgc cgtattatca
    18001 gggtattggc ccggcgtgga cgaaagagag cgtgctgccg ggaggtgcca ttgaaggcga
    18061 ccgcgacgat tacgccgctc gcctgcgccg ccgttacccg ttcctgactg aatcgctggc
    18121 acgtcactac gcgcgcactt acggcagcaa cagcgaactg ctgctcggca atgcgggagc
    18181 gataagcgac ctcggggaag atttcggtca tgagttctac gaagcggaac tgaaatatct
    18241 ggtcgaccac gaatgggtcc gccgcgccga cgatgccctg tggcgtcgca caaaacaagg
    18301 catgtggctg aatgcggatc aacaatctcg tgtgagtcag tggctggtgg agtatacgca
    18361 gcagaagttg tcgctggcgt cgtaaattaa cgtaaggtgg ccaggtcaga tttcaatctg
    18421 acctgagact gatgataaac aaaaaaactg cctgatgcgc tacgcttatc aggcctacgt
    18481 ggtttatgca atatattgaa tttgcatgat tttgtagacc aataagacgt tcacgtcgca
    18541 tccggcatgg acaaagcgca tatacaaccg ataaccaaag ttgtaattcc agaagtgaaa
    18601 tacgcctgat catgagcggg atagagaaca gtatctcccc agaaaactca gcgtaataat
    18661 acaagcagcc agcgcaacca ccgtcggcca tatcaagaac ggtacaataa taaagccgag
    18721 aaataccaca cctagcagca aaacaccgtg aatttgttca tcgcgctaat tcctttccct
    18781 cgagggttat ttcattgtgg gatacatcgg cacataaccc atattttcca tcaaacttta
    18841 ccctaccgat ggctaaaaac gggctaatcc gattctgcac tgactcacac atcactggta
    18901 ttccacccgc aaaacagcca gggcattata acgacccgca gcagggcgtc gccctgtggt
    18961 acttacaggg agagacagaa aagtcacatc tgccgtctga gctgactcag cccggttttc
    19021 aactaactta aatggcacac caacatgcgc agcaagagcg ttcaagtcta cggattcctg
    19081 ttggctatcc ataatcataa tgccaacgtc ggggttatcc tccgcctgaa taatgctttg
    19141 ccgaccatta acatctctcg ttgatgaaat attgccatca aaaaccagcg tcattacggc
    19201 atcaatatct ttgttcgtac agtttatcgg aatagtggtt gccactggag aaacgcccgt
    19261 gggcttcact ccagcaccag cccgacttaa ttcgctggcc cagacatccg gcatattaac
    19321 ggtaaaacta gttccctcat taattgtgca accttgtgga acttcaatat ttaagttgcc
    19381 aatataagca ttaaatactg gaattggtcc gtgctctgtt ggaaactggc tgacatacaa
    19441 agaaaccagc aatacgttat ttattgatat atcggatatt atttttttat caagtctgat
    19501 ttttatataa ccttctgttc ctgaggccca acttgatggt ttacttctgt ctgctggata
    19561 attggtgcta tagttgtcaa aaggtactgc cttatctgtc attgtgccat ttttattata
    19621 gagctttaca tgtgtcgaga cagatatttc atgagtcaaa ggaataaata tatcgctccc
    19681 ctgatgatca ccagacgctc ctgacggatc aatcccagaa acaccagaaa aataaacctc
    19741 ataatcatcc agagaattac aataagcctt ataatgccct attttccagt cctgcgaggg
    19801 taatactgtc ccaggcgaat tattattaac atctgagaca attaccgtac caaaattata
    19861 agaaaatgtc gcaggagtag tattattacc tggtgcacca ggataacaaa aggcgcgatc
    19921 ccgtcccata tcttcagcat atactatgtt atagcagcta ctgactaaaa gaacaaacaa
    19981 tatacaaatc gcttttatat ttccccaaaa ataagtaagc atcatgcttc tccagttatc
    20041 catgtatcgc tactgcaccc ataatagcat tattttgtaa aacatttaat tttacgctcc
    20101 atacgggtaa ggcattattg taaagatcta gtgtttgcca gttaatctca caatatcctt
    20161 gccgctgaca ccaatcctgt tggcgatgac taaatggtgc aaccatgcca ccatttatgc
    20221 ttttaccatc aatagataaa ttatagagtg tcacatacca gggcgtaggg ttaaaaacgg
    20281 tgacagcttc attgtttcgc gaccagcgta actgttgata agcgtcaagt ggattacctg
    20341 ataacgtctc tgggcggtaa aatattttca tcctattacg tacagccacc tggagttgat
    20401 tttcattctt aagctgtgag ttttgttcct gagcaggtaa cgctgcaata ttcagtataa
    20461 ataaactctc acgatctata ggtaactgcc cccccacgcg attgattctt attttggtat
    20521 ttttatcttg tttaagtacc aataatggtg gtgtggcaat aaacgcctta tctggaagta
    20581 aaggctggga ttccagtgtc ccttttaact gacgtccatc atcaggtgtc acggaaattt
    20641 taaccagcat atctttactg gcagtatttt taagctcgac cgaaattgat gactgctttt
    20701 ctgggtaaac aaaacgagta ccgccaacaa caactcctgc ctgcgcattt gtgcaacata
    20761 ttaaaatgca tatcagcacg ttaatcataa gtgacgataa aggaaactga tgcattcgca
    20821 tctcctggtt gtacatcatt ttttatagct ttataagcag caaagaaatg caatgtatta
    20881 tctaccaatg gtgtaagggc ataacttgta ctctttatac cattactaat actttgtctg
    20941 ttgttatcaa aaatagccag tgctaaccca tcgactgagc cctcttccag tttatatagg
    21001 gctgcgtctt cactaactgg tattccatca aacgtgacat tggcatgact gacatcactg
    21061 ccacaacggg ttaactttac agagaaacca attttttcag taacactccc tgaatttctc
    21121 accatatagc tgggaacatc aggcatgtta actgtgaaat ttttagattc atcggcaaca
    21181 atacaagtct ttgccgttat gattccttct atacgtacaa cacctgtatg tgttgtatca
    21241 tcagcaatgg atgacataga tatcacagag aataataggg caattaaatt ccaatatcgt
    21301 gtcatattaa cctcactgat agtctatatc aaagtacaat agtgcgttcg cctgtcccgc
    21361 atgtacgttt tgctgagttg acttatagcg gagtttaaag gttaaactgg catcgccatt
    21421 attatttaaa attaactctt tgaatcctga ttcactatta attgctataa ttttatcatt
    21481 actgtctaaa atttcgatgc ccactccagt tgcactatcc gcgctggaag taaggcgtaa
    21541 taaagtggta tttatagcat cagcatcccc ggtaaactgc acattagcac ttactcctga
    21601 actataaaca gtactggttg ttgtaggaca tccttccaga ttgatcgtaa atgtatggct
    21661 gccagaaaca tcccctacat tcaaaaaatc attgacgtca taagttccca tgtttactgg
    21721 ctggtcttta cttccagttg taatatcaca cgttttcgct aataattgtc ctgtaaatgt
    21781 gaccactaca gtatcgttag ccagtgcgtt atatgaaatc accatcatga acaatgatct
    21841 aaacattaat agaataaatt tttgtaatat attcattagc ggcactccag atttgcatag
    21901 ctaatggcat tatccaactc actttccggt aattgatagt ttgcccgaca ttgctgataa
    21961 gaactttctc cccactgtat atttattatg ccttgtaaag gcaaaccgga gagataaacc
    22021 tgtccactgt cacccactat tcctgtaatc tgagttgtcc cctcagtggg tacactgacc
    22081 gtcgcaccaa atggaactgg tttattacga tagtgaacat taaataaacc tcgaataccg
    22141 acatgtgcat taaactcagc acgaactaaa gcacctttgc tgggtatcac ttccatagta
    22201 gtagcatcta attctgtatg catactcata gacatagggt ctaaagagat attattacgc
    22261 cgatattccg tggcataagg tacgacagca tagccacgcc agtctgtttt aacccccgtt
    22321 ccgcgttcca catttacatt gtttgcccca ggtgctttta tcagtatatt tgtttctcct
    22381 aacggctggc ttaacgtgag accattttcg tgaataacca gggcaccact taaaccatag
    22441 ttaaattgct ggttaccatt attatcgtag ctgtatccag aatttagtga accatatgta
    22501 ccacggtaat ttatccctac cgagccattt tcgccattat tctggttggc aaaattttgt
    22561 tgaatattat agttgagatt atgttgttcc atcagcgatc cagatactcc ggcatactga
    22621 tttacatgac cgctattatc ctgactataa ccataagttg ctgtcataga atttgtcgca
    22681 gtacgcccat cgacagctag tgataaccaa ttgctgatcg gcaatgagac atttacggat
    22741 aagattctgt ccgcatctga acctggtgac ttactataat tataagcaat atttaaataa
    22801 atcccatgta cactcgtatt atagccgacc tgatacaagg tatctttatt attcgtattc
    22861 cagtaagttt gttgacttgc cgccaaatac atagagccat attcgcccaa tggttgcgat
    22921 atatttgcct gaaactttcc tcttttgtta taacgtaaat tataaaaatc attccagtta
    22981 taaacatagt ctttttcatt aagggtatct ggatcgataa ccgtacccga catttgtttg
    23041 tatgtggtat cactcaacga ataaaatccc tccgtcgaat agcgataacc aataatctga
    23101 aaagccgtac ctgtctgcac taacgatttt gaatatagga aacggtacga ttgcccttga
    23161 tgtttactgc catcggcaag cgtgctattg gcttgcgtta aatcaaatga cgtcgcacca
    23221 aacataccaa ggttgaaccc aagcccgagt gctattgccc gataattatt tgagtattgc
    23281 atccctccgt aggcggtaat atcccagggt aacccccaaa tcaaatctcc ttgaaagaca
    23341 ttcggactgt tttgattata gctattgcta cgatatttac cggcagttgc cgaatattta
    23401 atatgccctt cgcgttgaag aatgggaaca gcggcgtaag gaatggtata gatagtttcg
    23461 gaattatcag actctttgat cgtaacttcc aaatccccag cggaagacgt agggttcaga
    23521 tcgttgattt caaagggtcc cggtggagta tatgtctgat aaataacata gccattttgt
    23581 ttgatcgtta cctgagcatt gcttttagca ataccgcgca cagtgggggc aaaaccactt
    23641 tggctatcgg gtagcatgtt ttcatcggat tcaagtttag caccacgaat actcacactg
    23701 tcgaaaacat ccgatgaaga ccaggtatca cctagtgtta attcactttt aaaaggaata
    23761 attactcgtt gtaacgtact actcacatgt gcaattttac ccggtgaatt acctgaacga
    23821 ctccatgtag aataatcgcg aaaacgccac gcccctaaat ttaaacctga acggagattt
    23881 aaataataac tatcagacgc atagtctcca cgatcactgt tgccagaaaa atcataatta
    23941 ataagaatgg ccgggatacc attatcccat tcctcaggtg gaatataacc acgggcttca
    24001 tgctttaata ttgcctgcgg aaaggtcagt ttgagtcgtt gcgcatccag ttcaggagtg
    24061 tattgaactt ctgcgaacca tatacgtaaa tcgagacaat tatttccctc tgcttgcgcc
    24121 tgttccggca atgcactttt gttaattcct aaagtaacta actcatcgag cgaaaaacag
    24181 gggatcaact gattctgctc atcagctttg aaagtaacct tacgtgatgt caaaggcgaa
    24241 tcattgaccc atatttcaac ctcataacta ccaggtagat atgtcatccc tttctcaaaa
    24301 cgagataaat cagcaacagc ttctgtatca cccgacagga atgcagggtt aaagcgcata
    24361 cccgccactg aacacgcagg caaaagtgtc agtagaagca gcatacacca cctcatctgc
    24421 ttctgccttg ccatcctgtg ttcgagtttt ctgagcttta ggttcatatc cctgacctga
    24481 gttgctttaa cgttttaatc ttccaggttt attgcatcgt tcccttttgc ctggccgttg
    24541 tggcaccgta atcattgata gtttgataag taatttcacc ggctaacgtc actggtgaag
    24601 acactgttac gtcacttttc ggtggaacaa acgcattagg taattttgcc ccttgcaccc
    24661 tgattccagt caccgtcaga taatagggag aagggttaat gactctcagc tgtttacctt
    24721 cgtatttaaa tcgcagcata tcccgtgctt taccagcttg aacgggtaaa ttagtcggac
    24781 ggtaaaataa tttaattctg ttttgtaacg ccagttgtaa cgtgtttttc tgggtattct
    24841 tctcatccgt tggcggaatc gctttcacat tcatccagaa taatgtttcg cgatcagtcg
    24901 gtaactgtga tttaccaata aacattatcc tcaatgaggc ttcttttcgc gttggtaaca
    24961 taaataaagg aggcgtaacc acaaaatcct gacttttttt actgctttga ttgtcatcaa
    25021 tccatgactg gattaaatag ctggtgttag ccccaccatt ctgtacactt agcgtaattt
    25081 gctgactgtc agatggataa accagacgcg tagaaccaag accgaagcca cccgcagcaa
    25141 tagcattgat agatatggcg gatagtaaaa atagagcacc ggcccttatg atatattggg
    25201 tcttcagcaa agaattaatg tttctattcg aaaaacattt attttttgaa atccatctca
    25261 tactgcatcc ttgtagatga aattaattgt aattactgat gcatgtctat ttattacaaa
    25321 taaacatgta atattaattt caggttacaa gtaactgtaa cctgaaatta tcttgctgta
    25381 attattcgta cagcacgacg aaacttgccg ttgatttcac cagacctggt gcaacggtac
    25441 taccgttctg agcatagccc gcggtaaatg ccgcagtaaa atcatgagtg gctgcatcgc
    25501 caggaatcac gtaggctgct gaagaactac tcccctgggg agtttgtgtg ttcagcttaa
    25561 tcaggccatc agttgtcgtg tttttagaat aaatacgaat ccctacaccc gtagctcccg
    25621 cttcgccagt accatcagca tcagcagctc cttccacttc attggcaagc gtctggttgg
    25681 caccgtctgc tacgccagta aaacgaaatt caatattttg actgtccgta ttgctacaat
    25741 cagtcagttt aatgttgaag tcagtctggt tactggcacc cgttgcttcg gtatcaatgc
    25801 ccgtgaaagc gttagaaggt acgactggca gtaccacttc aatctgtttc gattctgtag
    25861 caaccttaca cgtcgcttta accacacgac cagtaaagcg gatgtttccg cccatcgcat
    25921 cggtgttgct atccatatcg gtgagttttg gataatcaac ggtgctccag tctgtatccg
    25981 caccaccaaa ttgtgatgca tcgcctggcg cagcggttac cgcagttgac atcagcatac
    26041 ctgatgcgag taaaactgcg gataaattag atagattgaa tttcatttat acctttcctt
    26101 ttgttaaaaa atccaagcaa tattggcgat tatttttaca ttcagcttta
//
LOCUS       DAYPHN010000061        25794 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000061 DAYPHN010000000
VERSION     DAYPHN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.061"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..70)
                     /locus_tag="ACG0BW_002347"
     CDS             complement(<1..70)
                     /locus_tag="ACG0BW_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259298.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPE"
     gene            complement(111..599)
                     /gene="ybjO"
                     /locus_tag="ACG0BW_002348"
     CDS             complement(111..599)
                     /gene="ybjO"
                     /locus_tag="ACG0BW_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFK7259299.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(659..1504)
                     /gene="potI"
                     /locus_tag="ACG0BW_002349"
     CDS             complement(659..1504)
                     /gene="potI"
                     /locus_tag="ACG0BW_002349"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFK7259300.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGKFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(1501..2445)
                     /gene="potH"
                     /locus_tag="ACG0BW_002350"
     CDS             complement(1501..2445)
                     /gene="potH"
                     /locus_tag="ACG0BW_002350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFK7259301.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(2455..3588)
                     /gene="potG"
                     /locus_tag="ACG0BW_002351"
     CDS             complement(2455..3588)
                     /gene="potG"
                     /locus_tag="ACG0BW_002351"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFK7259302.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(3683..4795)
                     /gene="potF"
                     /locus_tag="ACG0BW_002352"
     CDS             complement(3683..4795)
                     /gene="potF"
                     /locus_tag="ACG0BW_002352"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFK7259303.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKEATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(5146..5622)
                     /gene="ybjN"
                     /locus_tag="ACG0BW_002353"
     CDS             complement(5146..5622)
                     /gene="ybjN"
                     /locus_tag="ACG0BW_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFK7259304.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(5710..6612)
                     /gene="rimK"
                     /locus_tag="ACG0BW_002354"
     CDS             complement(5710..6612)
                     /gene="rimK"
                     /locus_tag="ACG0BW_002354"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFK7259305.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(6673..7395)
                     /gene="nfsA"
                     /locus_tag="ACG0BW_002355"
     CDS             complement(6673..7395)
                     /gene="nfsA"
                     /locus_tag="ACG0BW_002355"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFK7259306.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(7379..7666)
                     /gene="ybjC"
                     /locus_tag="ACG0BW_002356"
     CDS             complement(7379..7666)
                     /gene="ybjC"
                     /locus_tag="ACG0BW_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFK7259307.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            7826..8083
                     /gene="grxA"
                     /locus_tag="ACG0BW_002357"
     CDS             7826..8083
                     /gene="grxA"
                     /locus_tag="ACG0BW_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFK7259308.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(8113..8490)
                     /gene="ybjM"
                     /locus_tag="ACG0BW_002358"
     CDS             complement(8113..8490)
                     /gene="ybjM"
                     /locus_tag="ACG0BW_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFK7259309.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            8760..10445
                     /gene="ybjL"
                     /locus_tag="ACG0BW_002359"
     CDS             8760..10445
                     /gene="ybjL"
                     /locus_tag="ACG0BW_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFK7259310.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(10620..11156)
                     /gene="rcdA"
                     /locus_tag="ACG0BW_002360"
     CDS             complement(10620..11156)
                     /gene="rcdA"
                     /locus_tag="ACG0BW_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFK7259311.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            11240..12448
                     /gene="ybjJ"
                     /locus_tag="ACG0BW_002361"
     CDS             11240..12448
                     /gene="ybjJ"
                     /locus_tag="ACG0BW_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259312.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            12448..13263
                     /gene="ybjI"
                     /locus_tag="ACG0BW_002362"
     CDS             12448..13263
                     /gene="ybjI"
                     /locus_tag="ACG0BW_002362"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFK7259313.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            13348..13632
                     /gene="ybjH"
                     /locus_tag="ACG0BW_002363"
     CDS             13348..13632
                     /gene="ybjH"
                     /locus_tag="ACG0BW_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFK7259314.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(13673..14905)
                     /gene="mdfA"
                     /locus_tag="ACG0BW_002364"
     CDS             complement(13673..14905)
                     /gene="mdfA"
                     /locus_tag="ACG0BW_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFK7259315.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMAAGLSLYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            15190..15786
                     /gene="ybjG"
                     /locus_tag="ACG0BW_002365"
     CDS             15190..15786
                     /gene="ybjG"
                     /locus_tag="ACG0BW_002365"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFK7259316.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAI
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            15844..16602
                     /gene="deoR"
                     /locus_tag="ACG0BW_002366"
     CDS             15844..16602
                     /gene="deoR"
                     /locus_tag="ACG0BW_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFK7259317.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIMVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(16649..17851)
                     /gene="dacC"
                     /locus_tag="ACG0BW_002367"
     CDS             complement(16649..17851)
                     /gene="dacC"
                     /locus_tag="ACG0BW_002367"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFK7259318.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            18098..18724
                     /gene="gstB"
                     /locus_tag="ACG0BW_002368"
     CDS             18098..18724
                     /gene="gstB"
                     /locus_tag="ACG0BW_002368"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFK7259319.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(18721..19836)
                     /gene="yliI"
                     /locus_tag="ACG0BW_002369"
     CDS             complement(18721..19836)
                     /gene="yliI"
                     /locus_tag="ACG0BW_002369"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFK7259320.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRSTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(19947..20330)
                     /gene="bssR"
                     /locus_tag="ACG0BW_002370"
     CDS             complement(19947..20330)
                     /gene="bssR"
                     /locus_tag="ACG0BW_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFK7259321.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            20543..21868
                     /gene="rimO"
                     /locus_tag="ACG0BW_002371"
     CDS             20543..21868
                     /gene="rimO"
                     /locus_tag="ACG0BW_002371"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFK7259322.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            22094..22480
                     /locus_tag="ACG0BW_002372"
     CDS             22094..22480
                     /locus_tag="ACG0BW_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFK7259323.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            22470..22799
                     /locus_tag="ACG0BW_002373"
     CDS             22470..22799
                     /locus_tag="ACG0BW_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7259324.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(22869..24197)
                     /gene="dgcI"
                     /locus_tag="ACG0BW_002374"
     CDS             complement(22869..24197)
                     /gene="dgcI"
                     /locus_tag="ACG0BW_002374"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFK7259325.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(24205..>25794)
                     /locus_tag="ACG0BW_002375"
     CDS             complement(24205..>25794)
                     /locus_tag="ACG0BW_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFK7259326.1"
                     /translation="EYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIV
                     LLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKKSIAE
                     MKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGV
                     DERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFG
                     YRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIW
                     IARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDI
                     GFINELGYQIIKMAMGEFRHFSERAVLKDGFLLHINVSPWQLNEPHFHERFTTIMKEN
                     GLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPD
                     SIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQG
                     YLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         6617 a         6625 c         6172 g         6380 t
ORIGIN      
        1 gctctggaat cggctgcgtt atccactgac aggaacgaca gcgacccgcg tcgtaaagtg
       61 cgcactgcat acattgacct tcacatcatc aggggcgacg attatacaca ttattgcaac
      121 tggaagaacc gccgactggt cgagggaacg aacagcagca tcaggatgag catatccggc
      181 agcttctgca gcatcaggct atggaagatt tcacgttttg attcaccggg aatgctgaac
      241 agctccggat aaccataccc cagcgaagcc gcccacaagt aacttgcggc cgtgatttgt
      301 gtcaacagat acagccagcg cgcccagcga cgccctttca ccagtgaaaa cgcacaccag
      361 atctcaataa aaaccaacac cagactgctt aaaaagacca gcgttaaact ccaggtttgt
      421 acgctgcgat gaatgaactc gccaataccg cgcacgccca gcgtattgaa aatcatcagc
      481 acgtcgaggc cacggatcat aataatggcg agcgccgcca cctgcaccag cgcaggcaca
      541 ttcaggcgag catgagatga agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg
      601 aaaacaatgc cgcgacatgc gcggcattat gtagccaggt tggcaaattt tagtgtcttc
      661 agccacgtct tgcacgctgg atgtcgcgta tccgctgttt ttctgcgcga gccatcagat
      721 accaggcgat aaatccgaca attccgaccg caccgaggat caatgttgcc agggcgttga
      781 tttccggatt cacccccatc cgcacgctag aaaagaccag catcggtaac gtggtggctc
      841 ccggcccgga aacaaagctg gcgataacca gatcatcaag cgacaaagta aaagctagta
      901 accagccaga aatgatcgcg ggcatgatca tcggtagcgt aatgacgaaa aacactttca
      961 gtggcgtcgc accaagatcc atcgctgctt cttctatcga gctatccagt tcccgcagac
     1021 gcgacgaaat aacgactgcc acataagccg tacagaaagt gacatgcgcc agccagatgg
     1081 tgagcatacc gcggtccgca ggccagccaa tggcatgagc aagcgcgacg aataacaaca
     1141 atagcgacaa gcccgtgatg acatctggca tcaccagcgg cgcggtgatc ataaaggcaa
     1201 aaccatttga tccgcgaaac ttgccaaagc gcaccagcac caccgccgca atcgtcccga
     1261 ggatcgccgc cgccgtcgcc gcacatgccg caattgtcag gcttaaacca accgcactca
     1321 tcatcgcgtc atcgcgcaat aattcaccat accagcgcgt tgaccagccg gcccacaccg
     1381 tcaccagctt cgagctgtta aacgaataga taaccagcat cagcattggc gcgtagagaa
     1441 aggtgaagcc cagcagcaaa atcacaatcc gccagggcga acgaactacc ggtaaattat
     1501 tcatccgtgt tctcccacgc ttttttgctg gtgtttgtgg aaccacatta tcggcacaat
     1561 tagcagcagc aacatgatga tcgctaccgc cgaggccacc ggccagtcgc ggttgttaaa
     1621 gaactcctgc cacagcacgc gcccgatcat gatgctgtcc gggccaccga gcagttccgg
     1681 gatcacaaac tcgcccacag ccgggataaa caccagcatc gatccggcaa taatcccgcc
     1741 tttggtcagc ggcacgatca cagtaaagaa cgttttcagc ggtcttgcgc cgagatccag
     1801 tgccgcttcc accagcgaat aatcaatacg aatcaaggcg gtgtagatcg gcagaaccat
     1861 aaacggcacg taggcataaa caatgccgat ataaacggcc agattggtat gcagaatggt
     1921 cagcggctgg tcgataaccc ccagccacag cagaaaatta ttcagtacgc cgttattttt
     1981 taatattccc atccaggcat aaacgcgaat cagaaacgag gtccacgacg gcaggatcac
     2041 cagtagcaat aagatattac gggtcgaagg cttactgtgc gccaccgccc acgccagcgg
     2101 atagccgatc agtaaacagc aaatagtcga aatcgccgcc acctgtagcg actggagata
     2161 agcatcgaaa tagagcggat cgtcggtcag ttgcaggaaa ttaccgagat taagagtgat
     2221 ggaaagttgc ccgtcagccc actccatcag ttcggtataa ggtggaatgg cgcgcgccat
     2281 ctccgccagg cttattttaa agacaatcag aaatggcagc agaaacagca ggattagcca
     2341 gatatagggc agcgcaatca ccagtttgcg cccatgcttc atttgcaact gcgacagcca
     2401 cagcttaaag ggttttggct gggcagcagg ttcaagtgta ctcatctttg ctccttaaac
     2461 cgtcagcacc acacagctgt ccacttccca gcacaaacgc acttcgtcgc cccaggtcgg
     2521 taacccttta cgatgacgat gggcgttttg tagctgggcg ctgatcatct gcccactttt
     2581 cagacgaacg tgatacaccg aaagatcgcc gagataggct atgtgtatca cctcccccac
     2641 cgcgaagtta caaccattgg cgggcggctc ttcgcaaagc atgatttttt ccggacgcag
     2701 cgctacgtgt accggcacgt tatcaaccac tgaagcatct gcatcgactt tcagtgggtg
     2761 caccagtccc ggcgaataaa gcaccagacc atcttcctga cgctctttga gtacaccttc
     2821 aaagacatta accgagccaa taaattcggc gctgtagcgg gtggtcggat gctcatagat
     2881 ctcttccggt tcgccaatct ggacaaattt cccacgattc ataatggcga tacgccccgc
     2941 catggtcatc gcctcttcct gatcgtgggt gaccatcaca caagtcacac cgacacgctc
     3001 cagaatatcc accacttcaa gctgcatccg gtcacgcagc tttttatcca gcgcgcccat
     3061 cggctcatcg agcagtaata gtttcgggcg cttggcaagg cttcgggcca gggccacacg
     3121 ctgtcgctga ccaccggaaa gctgatgcgg tttgcgtttg gcgaactcct gcatatgcac
     3181 cagcccgagc atctcattga cccggctggc aatttccgct ttcggtagtt tgtcctgttt
     3241 caggccaaaa gcgatgttct gttccacggt catatgcgga aacagcgcgt aagactgaaa
     3301 catcatattg atggggcgca ggtaaggcgg aacctgtgac aaatcgacgc catcaagcat
     3361 tatctgtccg gcagaaggtt gttcgaaacc tgccagcata cgcagcagcg tggacttgcc
     3421 acagccggat gcgcccagca gcgcgaagat ttcacctttg tagatggtca ggctgacatc
     3481 atccaccgca tgttgaccat cgtaggattt ggtgaggttg cggatttcta atagcggcgt
     3541 cagcgcctta cgggttttcg cctgcgggcg agggatagcg tcattcacgg ctgctctccg
     3601 gcattgaatg tgtgcaagcg caccataatg gtgcgaattg ccggcgggtg tggtgcgccc
     3661 gtccggcatc tacgactgcg gattattttc cgctcttcac tttggtccac gcgcgggtgc
     3721 gcacacggtc tatttttgga tcctgcactt tcagagtgaa caacttcgca cgaacgtccg
     3781 ccggaggata aatccctgga ttatcacgca cttccgcgct taccagcggc gtagcttctt
     3841 tgttcgcgtt ggcatagaac acatggtcgg aaatatgcgc caccacatcc gggcgcagca
     3901 ggtaattcag gaactgatag gcttcgtctt tatttttggc atccgcaggc atggcgaaga
     3961 catcaaagaa cgccatcgcc ccttctttcg gaatcgagaa cgagacatta acgccatttt
     4021 tcgcttcctt cgcgcggttt gacgcctgcc agacatcgcc cgcccagcca atagcgacgc
     4081 agatatcgcc gtttgccagg tcgttaatgt attgagatga atggaaataa cgaatgttcg
     4141 ggcgcagctt taacagcaga tcggttgccg gtccggtgta atcatctgct ttagtgctgt
     4201 tgggatcttt accgagataa ttcagcacgg tagcaaacac ttcttccggt gcatcaagga
     4261 aagagacgcc gcagcttttc agtttttcca gattttcagg cttgaggatc aagtcccagc
     4321 tatcgacggg cgcgttttcg cccagcaccg ctttaacttt atcaacgtta tagccaatcc
     4381 cggtcgtcgc ccacatatag ggcatagcaa atttattgtc gggatcgtgt ttggcaacca
     4441 acttcagcag ctccggatcg agattcttcc actccggcaa tttgcttttg tccagcggct
     4501 ggaaaactcc cgcagtcaac tggcgttcaa gaaagcttgc tgacggcacc accaggtcaa
     4561 agccggtgct acccgccatt aatttgcctt ccagtacttc gttagagtcg aaaacatcgt
     4621 agacgacttt aataccggtt tctttttcaa aattggccac cgtgtccggg gcgatataat
     4681 cagaccagtt ataaatgtgg agtgtttttt gttcagccgc gagcgtgccg acagagacgg
     4741 ccatcagagc acccgcaacc agacccgata gccatttttt atttaaggcg gtcatatctc
     4801 tttccttctg aaagttcgtt aacaaacgaa caaaagttgt cacactgaaa gatatgcaat
     4861 aaacgtgcat attttttatc ggcgcggtgt aagaaaagag aactctctcc cggccagcac
     4921 tgctcgctat ataaaagcat cgcaagaata actttagcca gggtttgaga ctatagctcg
     4981 gattaaaaaa cggtatttaa caaattttta tgcgtatttg ctctatgtga taaagccaaa
     5041 tcggcacatg agaagtgaaa taatctttaa gaaaaggtta aaaacagcag ataattttgc
     5101 agcacgcagt cactgcggca gcgactgcgt actcgaaagg agttatcagt ggaggaaata
     5161 gttttcggta acgttgtttt gcccttcatc gtcagtcggt aacagcagtt gatgggcatt
     5221 agcttcaaga atgaccatcg aaatctgctc ttcgctctga cgcacgaacc atgcaaactg
     5281 ctcataggtt acgccctgca taacggataa agactggcaa accaccagct ttggcagatt
     5341 atcatcctgc atatcaagaa atgctttcac ggtcagcgaa ctggcattga tggcagataa
     5401 atctgccgcc aggggcaata cggctgaagg tcggacttcc gccatggcag aaaacaggat
     5461 cgtgttatcg atcagatcga ttttggcatc aaagacaccg tcgaaattct gcatatgggg
     5521 cagatgcaga gcctgacagt tatcacattc aaaaaaactc atccccaggt catcgagcca
     5581 ttgacgcagc gtatccagac caggaacgac cagcgatgtc ataattttgt acaacctctt
     5641 tcgataaaaa gaccggcaca gcttacgcaa aaagcgcagg caaaaccatg atcagtaatg
     5701 tgattgcgac taaccacccg ttttcaggca atattctgtc gtagcgtggc gttcgatcca
     5761 gcggatcatt ttacccgcga tgtcgatacc ggtggttttt tctattcctt ccagccccgg
     5821 cgacgcattc acttccatca ccaacggccc gcgattagca cgcaaaatat ctacaccagc
     5881 aacatccagc gccatcgttc gcgcggcttt tatcgcgatt tcacgctcct gtggtgtgat
     5941 actggcgacg cttgccgcgc cgccacgatg caaattggaa cgaaaatcgc cctctttcgc
     6001 ccgccgttca atcgcagcga ccacttcatc gccaacaacc agacagcgga tatcgcaccc
     6061 ttgcgcctct ttgatatatt cctgcaccag aatatgcgcg ttcagaccgc ggaaagcgtc
     6121 aatcacgctt tcggccgcct gacgcgtctc cgccagcacg acgccaattc cctgcgtgcc
     6181 ttcaaccaac ttgaccacca gcggcgcacc accgaccatg tcgattaaat cgctggtatc
     6241 atccggcgaa tgcgcaatgc ccgtgacagg caggtcgatg ccctgacgcg ccagcagttg
     6301 catggaacgc aatttgtcac gcgcccgggc aatggcgacc gactcattga gcggatagct
     6361 ccccagcatt tcgaactggc gcagtgccgc cgtcccataa aaggtaatgg cggtaccaat
     6421 acgcgggatc actgcgtcaa aatggggtaa cttgcggcct ttgtagtgaa tagaagacgc
     6481 cgcaggattt atgttcatgt agcaagaaag cggatcaaga atttcaacca ggtgaccacg
     6541 ctgtatagcg gcttcacgca gccgcttaca cgaatagagc gttccatccc gggacaatat
     6601 ggcaattttc accctgcacc tctctgtcag acctggtcaa aagcccgcgt atcatacatc
     6661 ggcgtggcgg ttttagcgcg tcgcccaacc ctgtttgtgc aaataatcca gtataaatgg
     6721 gcggctttct ttaatgattg ttcggcggat atgatcgctc caggtatccc ggcgattatt
     6781 gctgccacgt gtgaggtaat attccgccag ttgttcgtca tactgcgcca gtgcgtcttt
     6841 atccagcggt tgatagctgt tttcatgcac caaaatggag gacggtaaac gcggcttaag
     6901 atccggatta tccgcaggcc agccaaggca cagcccaaac agcggcagaa catgctgcgg
     6961 taatttcagc agctccgtca ccgcttcaat attattgcgc aggccgccga tatatacccc
     7021 gcccaatccc agcgattccg ctgcggttaa tgcattctgc gccatcattg ccgtatcaac
     7081 gacaccgagc aacagttgtt ccgccaggcc aagttgtgca tccggacaga tctgtaaatg
     7141 gcggttaaag tcggcacaga acacccagaa ctccgccgct tgcgctacgt gtttttgccc
     7201 gccggtcagc gtcaccagtt catcacgtaa cgctttgtcg gtaatgcgaa taatgctact
     7261 gcactgcaaa aaactggaac tggacgtcgc acgggcgctg ttaataatcg cctcacgctg
     7321 cgcttcggaa atgggttcat cagtgaaatg gcgaatggag cgatggccac aaataagttc
     7381 aatggttggc gtcattatct ttttctcttt ctgaacgtga atattgcggt ggacggttca
     7441 tcagctgtgg ggcaagacgt tttgccacct gaagaataac caccaccgca gcgggaagca
     7501 tgagcaaaac accgagaaaa atcatcagaa tctgcacttc tggccgagaa aatggctcag
     7561 gcagcgacag ggagccgctt accgacagca gcgccaccgc cagcaacatc attccgataa
     7621 attccagtat caacacgctt ttaggcaatt taccgatcgc gcgcatacgc ttccctctgc
     7681 aaagtgagcc ttcagtctaa aacttttcac tgtattgtgt ttaacagtta tagcttttag
     7741 caattaatgc aacaggttaa acctactttc agcgaattca ttttagcgtg atcattacag
     7801 gcataaatct atgaggagag aaataatgca aaccgttatt tttggtcgtc cgggttgccc
     7861 ttactgtgtg cgtgcaaaag atctggctga gaaattgagc aatgaacgcg atgattttca
     7921 gtatcagtat gtagatattc gtgcggaagg gatcactaaa gaagatctac aacaaaaggc
     7981 aggtaaaccc gtagaaaccg tgccgcagat ttttgtcgat cagcaacata tcggcggcta
     8041 taccgatttt gctgcatggg tgaaagagaa tctggacgcc tgatcgtctg acaagccctc
     8101 gcgttgaggg ctttattgat tttttctgtg ctgtggttta aacaaactac tgataaataa
     8161 gaaacacagt gcccccagcg cacaccagaa caccgcactt aataaccatg ccagctcttg
     8221 ccagaatgag cgcgtcggtg aaaaaaacag ccgcataata agcattgaac agggtgccgc
     8281 cagcattgcg ccaaacagag gtttcaagac ttctctacgc tgtgaaaaga agctggcaac
     8341 tgctccagga agaatgaaaa acagcaagcc gatttcagga tgcccggcag cccgaaaagc
     8401 gcctttcatg tgcgtcgcca gaaaaaggca caccacaatg aagaggacaa aacagcagat
     8461 tgcccccgcc caacgttgtt tatgtttcac tcgttcctcc tgacactgcg tctatcgaac
     8521 acatttttcg ccagtgtggc gttcagtaag ataaagccgc ttcgcattcc atgctaatat
     8581 aggccaacgc aattcatata gccgttgata cctaatgtga ttacactagt aaaatatatt
     8641 gttactttac tatcgtttag gtgcgctgaa tgaatctgcg ccctgaattc tggtaaaaaa
     8701 cattatcgta aattaccatt tctttcaaca gcttactagt aaacaagaag ttagcctccg
     8761 tgaatataaa cgtcgccgaa ttgttaaatg ggaattacat tctgttatta tttgtggtcc
     8821 tcgcgcttgg gctatgtctc gggaaattac gacttggttc gatccaactg ggtaattcca
     8881 ttggcgtttt agtcgtatcg ctgttattag gccaacaaca tttcagcatt aacaccgacg
     8941 cgcttaatct tggctttatg ctgtttattt tctgcgtcgg ggtcgaagcc ggaccgaact
     9001 ttttttccat tttttttcgc gatgggaaaa attacctaat gttagcactg gtgatggttg
     9061 gcagtgcgct ggtgatcgcc ttagggttag gtaagctgtt tggctgggat attggcctga
     9121 cggccggtat gttagcaggc tctatgacgt caacaccggt tttggtcggt gctggcgata
     9181 cactgcgtca ttccggcatg gaaagcaggc agctctcact ggcactggat aatctgagcc
     9241 tcgggtatgc cttaacctat ttaatcggtc tggtgagttt gattgttggt gcgcgttact
     9301 tgccgaaatt gcagcatcag gacttacaga ccagcgccca gcaaatcgcc cgcgaacgtg
     9361 gcctggacac agatgccaac cgtaaggttt atttgccggt gatccgcgcc taccgcgtcg
     9421 gcccggagct ggtagcctgg accgacggca aaaatctgcg tgaactgggt atttatcgac
     9481 aaaccggctg ctacattgaa cgtattcggc gtaacgggat tctggcaaat ccagacggtg
     9541 atgccgtgct acaaatgggc gatgaaatag cgttggtagg ctaccccgac gcccacgccc
     9601 gactcgatcc cagcttccgt aacggcaaag aagttttcga tcgtgacctt ctcgacatgc
     9661 gtatcgtcac tgaagaagtg gtcgttaaaa accataacgc tgttggcaaa cgcctggcac
     9721 aactgaagtt gaccgatcac ggttgcttcc ttaaccgcgt cattcgtagc cagattgaga
     9781 tgccgattga tgacaacgtc gtgcttaaca aaggtgacgt tttacaagtc agcggcgatg
     9841 cccgccgggt aaaaaccatc gccgatcgca tcggctttat ctcgattcac agccaggtca
     9901 ctgacttgct ggcattctgc gccttctttg ttattgggct gatgatcggg atgatcacct
     9961 tccagttcag cacattcagt ttcggcatgg gaaacgctgc cgggttgtta ttcgccggaa
    10021 ttatgctggg ctttatgcgt gctaaccacc cgaccttcgg ttacattccg cagggcgcat
    10081 taagcatggt gaaggagttc ggcttgatgg tgtttatggc aggcgttggt ctgagcgctg
    10141 gtagcggtat taataacggc ctgggcgcga ttggcggtca gatgttgatt gccggattga
    10201 ttgtcagtct ggtgccagtg gttatctgct tcttgttcgg tgcttatgta ttgcgaatga
    10261 accgcgcact gttgttcggc gcaatgatgg gcgcacgcac ctgcgcgccg gcaatggaga
    10321 tcatcagtga tacagctcgc agtaatatcc cggcgctggg ctatgcgggc acttacgcaa
    10381 tcgccaacgt cctgctgacg ctggcaggga caatcatcgt catggtatgg ccaggattag
    10441 gataaaactg aagttgccct gaaaatgaaa tttttttgca caaccgcaga acttttccgc
    10501 agggcatcag tcttaattag tgccactgct tttctttgat gtccccattt tgtggagccc
    10561 atcaaccccg ccatttcggt tcaaggttgg tgggtttttt gttgcctata atttatctac
    10621 taccctgcaa ccctctcaac catcctcaaa atctcctcgc gcgatagcgg cttacggtcg
    10681 gtgacaaaat gcagcgtcat cccctcaata aacgcatcaa gcgcgcgggc ggttccgggt
    10741 tcaaaccatt gttcgagcgt ttgctgactg cgctgcatcc agttttgcat taccgttttt
    10801 aatagcggtt ttcggctagc cagcgcgtag agctggtaca tcagctccat gttatccggc
    10861 gttgcaacct gtgagctgta gatcatatcg gtgatagcct ggcatgcgcc ttgagcatca
    10921 ctaacatcgc taaaaaatgc ctgatattgc cgggacatga tctcagtaaa actgctgaac
    10981 gcctccagca acaactcatc aattcctgaa aaatagtagg tcatcgaccc caacggtacc
    11041 ccggcaaggg tagcaatttt gcggtgcgta acagcatgta ttccgtaaag tttcaccgcc
    11101 tccagcgtgg cctggataat tttttctcgc cgttgcggat cgttagcgcg acgcatgtca
    11161 tttcctctct ctgcaattgt gtacaaatgt acacaacctt gctaatgttg tcttccctct
    11221 tcttattttg acgtgatcta tgaccgtaaa ttcttcacgt aatgcattga aacgccgaac
    11281 ctgggcgctg tttatgttct tctttttgcc gggcctgtta atggcgtcct gggcaacccg
    11341 tacgcctgct atccgcgata ttctttctgt ttcgatcgct gaaatgggcg gagtcctctt
    11401 tggtctgtcg atcggttcaa tgagcggtat tctctgctcg gcgtggttag tgaaacgctt
    11461 tgggacgcgt aatgtcatcc tggtcacgat gtcctgcgca ttgatcggga tgatgatact
    11521 aagtctggca ctctggctga catcgcccct gctcttcgcg gttggcctcg gcgtctttgg
    11581 ggcaagtttt ggttctgcgg aagtggcgat aaacgttgaa ggtgctgccg ttgagcgaga
    11641 aatgaataag acggttttgc cgatgatgca cggtttttat agcctgggca cgctggcagg
    11701 cgctggtgtc gggatggcac tgacggcctt tggcgttccg gcaacggtgc acattttatt
    11761 ggcggcgctg gtaggcatcg cgcctattta tatcgccatt caggcaatcc ctgacggtac
    11821 gggcaaaaat gctgccgatg gcacccagca tggtgaaaaa ggcgtacctt tttatcgcga
    11881 tatccagttg ctgctgattg gtgttgtggt gctggcgatg gcctttgccg aaggttctgc
    11941 caacgactgg ttacccttat taatggttga cggtcacggt tttagcccta cttccggctc
    12001 gctgatttat gccggtttta ccctggggat gactgttgga cgctttaccg gcggttggtt
    12061 catcgaccgt tacagtcgcg ttgccgtggt tcgggccagt gcactaatgg gagcgttggg
    12121 tattgggctg attatttttg tcgatagcgc ctgggtcgct ggggtgtctg ttgtactttg
    12181 gggactgggt gcctcgctgg gcttcccgct gaccatttct gccgccagcg ataccggccc
    12241 cgatgcacca acccgcgtca gtgtggtagc aacgaccggt tatctggctt tcctcgtcgg
    12301 gccgccactg ctgggctatc tcggcgaaca ttatggatta cgtagtgcaa tgctggttgt
    12361 actggcgctg gttattctcg cggctattgt cgcgaaagcc gtcgccaaac ccgataccaa
    12421 aacgcagacg gcgatggaga atagttgatg agcattaaat taattgcggt agacatggat
    12481 ggtactttct taagcgatca aaaaacctat aaccgtgagc ggtttatggc tcagtatcag
    12541 caaatgaaag cacaaggcat tcgctttgtg gtcgccagcg ggaatcaata ttatcagttg
    12601 atctctttct ttccggaaat tgctaatgaa atagcctttg tggctgaaaa cggcggctgg
    12661 gtagtgagcg aaggcaaaga tgtttttaat ggcgagctgt cgaaggatgc gtttgctact
    12721 gtcgtggaac atttgctgac gcgcccggaa gtggaaatta ttgcctgcgg aaaaaatagc
    12781 gcctataccc tcaaaaaata tgacgatgcc atgaaaacgg tggcggaaat gtattatcac
    12841 cgtctggaat acgtcgataa ctttgacaac ttagaagata tcttctttaa gtttggtctg
    12901 aatctttccg atgaactgat tccacaagta caaaaagcat tacatgaggc catcggcgat
    12961 attatggtgc cggtccacac cggcaacggc agtatcgatc tgattatccc cggcgtacat
    13021 aaagccaatg gccttcgcca actgcagaaa ttatggggaa tagacgacag cgaagtggtg
    13081 gtctttggcg atggcggtaa cgatattgag atgctgcgtc aggcaggctt tagttttgca
    13141 atggaaaatg ccggcagcgc ggtcgtcgca gcggcaaaat accgggcagg ctccaataac
    13201 cgtgaaggcg tactggatgt gatcgataaa gtgcttaaac acgaagcgcc atttaaccaa
    13261 taaaacggca gcacgcccgg caacacaata cctgaatgaa ggctgcataa ttcgcgcaaa
    13321 ttaacccgct gcattatttc aggcgaaatg attatgaaaa attgtctact gttgggcgcg
    13381 cttttaatgg gctttactgg tgtggcgatg gcgcaaagtg tcaccgtcga tgtgccgagt
    13441 ggatacaaag tggttgtggt tcctgattca gtcagcgttc cacaagcggt cagcgtcgcg
    13501 actgtgccgc aaaccgtcta tgtggctccc gcgccagctc cggcttaccg cccgcatcct
    13561 tatgttcgcc atcttgccag cgtaggtgaa gggatggtta tcgaacacca gattgacgac
    13621 catcaccatt aatttgagaa tgcctgatcg cacaaagcaa tcaggcattt ttttatcctt
    13681 cgtgcgaatt tcccatctgt ttatctttta aaaagataac catcagcaac agccacaaaa
    13741 ttccgttgac aaggttgaag agattaaaca gtccgttgcc cccgttcagc caggcatgtt
    13801 tgctgatttc aataccgacg gtaaagatca gcatttgcag catccccatc gccgcagaaa
    13861 ccgtaccttt actcatatcg ctggcaaaca gagttaatcg caccagtccc gcattcgcca
    13921 gaccaatacc gaaagcataa agacttaacc cggcggccat ccataaatac gcatgcgatg
    13981 agataaccgt tgccgcagca gcgaccaaca gaccaatcat aatcggccag ccgcccataa
    14041 taatcagcga acgtacggtg cggcgcgagg tcagacgcgc taacagcaag ttacccgcaa
    14101 ttaacgcccc gaaaataggc acttgcagca aaccatattc atagctgctt aactgctcgc
    14161 cggtaatgat gataatcggc gactgggcga tccacgccag caacggcaaa ctaacgaatc
    14221 ccagcgccag cgcccccgca acaaagcgac cgttcttcag caccagctta tagtcacgac
    14281 cgagttcttt cagcgacagt ttctcgccta tacgcgtggc ggtttcaggc atggctcgtt
    14341 gcagaccgaa aaaggagatc gctgccaatg cggcaaacaa gacaaacatc ccttcccagg
    14401 gcagcacatg gatccacgcc gcgcccacca gcgggccaag tagtggagca atcagcgcca
    14461 cgttcgccat cagcgcggtg atcttgatac aaaccgcctc ttcgaaggat tcctgaattg
    14521 cggcgtatcc cacagcgcca atgaaacaga ggcttatgcc ctgcaagaag cgcaacagag
    14581 tgaattgttc aatgttttgc gccagtaata ttgccagaca ggtgatgata aaccacacca
    14641 cccccgccag catcaccgga cggcgaccaa tacgatccga cagcggcccc aggagccatt
    14701 gtaaaaacat cccgcccgcc agatatgcgg tcatcgaagt aggaacccaa tcaatgcccg
    14761 cctgatattg ttccaccacg gccaacatac cgggttgaat catatcgttg ccgatatagg
    14821 ttgaaaattc gtacagcacc agacagagag ggaaaagtaa cgcctgacgt ccaagcctgg
    14881 caccggaagc taatttattt tgcatgcaat ttcttcgcca ataataatcg cgcaaagttt
    14941 aataaaagcg cagctaacga gaaagcgaat tttgtagctg aaaccacggt taagcacatt
    15001 cttacattat tgcgagtata gctacgcttt ctttaagttt tatttaacct atgcccgtta
    15061 caatcaccca ccgtaaacag gccgcttgag ggaaataaga cgatgccgct ttacccagtt
    15121 taacctgcac tttattctca acgacttgcc tgtattggct cccttttaat cactttgcgt
    15181 cgggaagtta tgctggaaaa tttgaatctc tctctattct ctcttattaa cgcgacgcca
    15241 gactcggctc cgtggatgat ctcgttggcg atttttattg ctaaagattt gattaccgtg
    15301 gtgccgttgc tggccgcgat actttggttg tgggggctta cagcacaacg gcaactggtg
    15361 ataaaaatcg ctattgcgct ggcggtcagc ctgtttgtgt cctggacgat gggacatctt
    15421 tttccgcacg accgcccctt tgtcgaaaat atcggctata acttcctgca tcatgcggcg
    15481 gatgactcat tcccgagcga tcacggtacg gtgattttca cctttgcact ggcattttta
    15541 tgctggcatc gcctgtggtc cggctcactt ttaatggtgc tggccgtcgt cattgcctgg
    15601 tcgcgcgttt atcttggcgt ccactggccg ctggatatgc tcggtggatt gctggcaggt
    15661 atgattggct gccttagcgc ccagattatc tggcaagcga tggggcataa actctatcaa
    15721 cgtctgcaat cgtggtatcg cttctgtttt gcattaccga tccgcaaagg ctgggtgcgt
    15781 gactgatctc tgctaaaaag tgtagtattg agcggctcgc ttcaataact attcagaggg
    15841 attatggaaa cacgtcgcga agagcgtatc gggcagctgc tgcaagaatt aaaacgcagc
    15901 gataagttac atcttaaaga cgccgccgcc ctgcttgggg tttcggagat gacgattcgt
    15961 cgcgatctga acaaccacag tgcgcccgtc gttttgctcg gcggctatat tgttctggaa
    16021 ccgcgcagtg ccagccatta tctgttaagc gatcaaaaat cccgcctggt ggaagaaaaa
    16081 cgccgggcgg caaaactggc tgcgacgctg gtagaacccg atcagaccct cttttttgac
    16141 tgtggcacca ccacaccgtg gattattgaa gcgattgata atgaaatccc tttcaccgcc
    16201 gtttgttatt cgctaaatac ctttctggca ctgaaagaga aacctcattg ccgcgcgttt
    16261 ctttgcggtg gtgaatttca cgccagcaac gccattttca aacccatcga ttttcagcaa
    16321 acgctgaata atttttgccc ggatatcgct ttttattccg cggcgggggt gcatgtcagt
    16381 aagggcgcta cctgttttaa tcttgaagag ttgccggtaa aacactgggc catgtcgatg
    16441 gcgcaaaagc atgtgctggt tgtcgaccac agtaaatttg gcaaggtgcg tccggcgcgc
    16501 atgggtgacc tgaaacgctt tgatattatg gtaagcgatt gttgcccgga agatgaatat
    16561 gtgaagtacg cgcagacgca gcgtattaag ttgatgtatt aatgacgtat aaccggatga
    16621 cgtttcgcgc catccggtta tcagaagatt aagagaacca gctgccgaac cactgatgga
    16681 atttcatcat cacgaaatcc cacatccgac caaagaatcc cccctcttcc acattttcca
    16741 tcacgatcag cggacgctgc tcaatggatt taccgttaag ctggaaatca atggtgccga
    16801 caacctgacc ttttttcagc ggtgcggtaa gctgcggttc cgttaacgtg tagctcgctt
    16861 tcaggttttt cagctgccca cgcggtatgg tcactgagcc cgcttcgcct gccccgagat
    16921 tcacttcgct cttatcacca aaccagacgc gctgagtcac aaaggtggca tcaggtttaa
    16981 ttggcgtcac ggtttcaaag aagcggaaac cccaggtcaa taatttctca gactcattaa
    17041 aacggatacg gtcagttttc gcacccagta ctacggagat caaacgcata tcgccctggg
    17101 tagccgaagc aaccagatta tatcccgcgc ctgccgtggt tcctgtcttc atgccatcaa
    17161 cattcagatt gctgctccat agcagacggt tacggttagg ctgtcgaatt ttgttgaagg
    17221 tgaactcttt ctctttatga atggcatact cttccggcac atcgtggatc aatgctttac
    17281 ccagcaatgc catatcgcgc gcggtactga actgccccgg cgcatccaga ccgtgcaccg
    17341 tctggaaggt agtgttggtc agacccagtt ttttggcata accattcatc agaccaataa
    17401 atgactcctg gctcccggca acgtaatcgg ccagcgcaat acaggcgtca ttaccggact
    17461 ggataatcac acctttgttc aggtctgcca ccgaaacctg atcgcccggt ttaaggaaca
    17521 ttaccgatga accacgcagt gccggatttc ccgtcgccca ggcatcttta ccgaccgtca
    17581 ccatatcggt gagtttaatc ttatcggcct taagcgcctg cccaaccaca tagctggtca
    17641 tgattttagt caggctcgcg gggtccagtt tttcatccgc attgccttct gccagcactt
    17701 taccgctggc gtaatccatt aaaatccatg cacgcgcatc cacgctcggc gcttcaacgg
    17761 tttgttccgc cgcgaatgcc gttggggcaa aaaggaataa aaatgcagaa cccgctgcaa
    17821 gaccacgaag gagagaggag tattgcgtca taaatgccac ccgagtatcc attcaaaaaa
    17881 aatacgttat atcgccgtat gataagttcg gtgattaata acgcacacac aaacttaaag
    17941 aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt cgctgcttct
    18001 ggtgtcattt ttttgccagc tgttgcttaa aagttaactt tacttcacca tcggagtcct
    18061 gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg attacgctgt ggggtcggaa
    18121 taattcaacc aacgtaaaaa aagtattgct gacgctcgaa gaactggaac taccttatga
    18181 gcaaattctc gcgggccgtg agtttgggat aaatcacgat gctgattttc tggcgatgaa
    18241 ccctaacggg ctggtgccgt tgttgcgtga cgacgaaagt gatctcattc tttgggaatc
    18301 aaacgccatt gtccgctatc tggcggcaca gtacgggcaa aaacgcctgt ggatcgactc
    18361 accggcacgt cgtgcggaag cagaaaaatg gatggactgg gcaaaccaga cgctcagtaa
    18421 tgcccatcgc gggatcctga tgggattagt cagaacacca ccggaagagc gcgatcaggc
    18481 cgccattgat gccagttgta aagagtgcga cgccctgttt gccctgctcg atgcggaact
    18541 ggcaaaagta aaatggttct ccggcgacga gtttggtgtg ggcgatatcg ctatcgcacc
    18601 gttcatctac aatttgttta acgttggcct gacctggaca ccgcgtccaa atctgcaacg
    18661 ctggtatcag caactcactg aacgcccagc agtgcgcaaa gtggtgatga ttcccgttag
    18721 ctaattgcgt gggctaactt taagtaattc cccactggac tcgtcggtga gaacgtataa
    18781 ataaccgtcg ggtccagtgc gaacatcacg aattcgctgc cctctgtccg ttaaaatacg
    18841 gccatcttct gtcactttgt cgccgttgac gctcatcaca atgacatctt tatctttcag
    18901 cgcgccaata aataattttt gctgccactg ggggaattta tcgctgttat agaaggccat
    18961 gccgctcacg gcgggcgaat ctttccagta aaaaacaggt tgctcggtcc cggcgacgat
    19021 ctcccctttc gcttccggta tcttaaagcc cgaatagttg attccccagg ttgccagcgg
    19081 ccagccgtag tttttgcctt tttgcgggat attaatttca tcaccaccgc gcgggccatg
    19141 ttcattcagc cacagtgcat tactccacgg attcatcgcc attccttgcg gattacgaat
    19201 gccataagac cagatctcgg cgcgcacacc ggattccttt ataaaaggat tatcatccgg
    19261 gatttcgccc aggtcggtca gacgcaccag tttgccctgt aatttatcca gatcctgcgc
    19321 cgtcgagcgc tgattgtttt cgcccagagc aacaaaaaga taacctttac cgtcgaatac
    19381 cagccgcccg ccaaaatggt tgccggtaga cagttttggc atctggcgaa agacggtgcg
    19441 gaagtcggtc acttttgaga gatcatcact taagcggcca taacccacag cagttccggc
    19501 tttgccatca tcgccaactt cggaataact taaccagatg cggcgagact gagcaaaatc
    19561 aggcgctaaa accacgtcca gcaggccgcc ctgcccgtgc gcccaaacgt ccggcactcc
    19621 ggaaagcggc gcagataatc cttttcctgc ttgccagtga cgcaactcgc cgccgcgcag
    19681 agtgattaac ataccgtgat tatcgggtaa aaaggccagc gcccagggat ggtcgagttt
    19741 gtcttgcagt acttcgacat ttaccgttgc aggagccgcc cagagagcgg aagaaagaca
    19801 aataaggggc acaaggaaaa aggattgtcg atgcataacg cctccggatt gactctggct
    19861 taaagcgtag tcagtggagg agataacaaa ttcattttta caaaaactta aacatgaagg
    19921 gggagacgct ttctccccct tagtttttag gccttctcaa gcatggcgtg cttctgcagg
    19981 ctctggatac tcagcgttaa gctcatcaga caattttcaa gcttatcggc gttgacggta
    20041 ataacagtcg ggcaatcatg gtgcccactc atcaaacata ctgcggctgt cgctaatgct
    20101 tcttcagcat gatgaagagc actccactct tcctgatcca gatgaagatt caaccgcagc
    20161 gatttatcgt gcagttcgcg attcagttta aaaaagttat ctcgtagatg attgctttcg
    20221 ctgacggaca tgtatccttt tgccttcctc agttgcacgt atgcggcgag gtcaacgcgt
    20281 gcgtcgatca accggttcag cagatcgatt cgctgtctgt caacgaacat acttcgatcc
    20341 tcctctttcc agccttgttg caattaaata gtatggctat tttttgagca aaatgctgtc
    20401 caggatcaat tcttcacgcc gaattaacaa aatttacatt tccttcgcct ccgccagcga
    20461 atgagattgc gatacaaatt ttatccagaa ctctgtagaa tccctgccct gacctttcct
    20521 gtaactgaat attttttaag ctatgagcaa agtaactccc cagccgaaaa tcggctttgt
    20581 ttcccttggc tgtccgaaaa accttgtcga ttcagagcgt attctcaccg aactacgcac
    20641 tgaaggttat gacgtggtac cgagctatga cgatgcggac atggtgatcg tcaacacctg
    20701 cggctttatt gacagcgcgg tacaagaatc actggaagcc attggtgaag cgttgaatga
    20761 aaacggcaag gtaattgtga ccggttgtct gggggcaaaa gaagatcaga tccgcgaagt
    20821 ccacccgaaa gtgctggaaa tcaccgggcc tcatagctat gagcaggttc tggagcacgt
    20881 tcatcactac gtgccaaaac cgaaacacaa cccattcctg agcctggtgc cagaacaagg
    20941 cgtgaaactg acgccgcgtc attatgccta tctgaaaatt tctgaaggct gtaatcaccg
    21001 ctgcaccttc tgcattattc cgtctatgcg cggcgacctg gtgagccgtc cgattggcga
    21061 agtgttaagt gaagcgaaac gtctggttga tgcgggcgtt aaagagattc tggtgatctc
    21121 gcaggatact tccgcctatg gcgtcgatgt taaacatcgt actggcttcc acaacggcga
    21181 gccggtaaaa accagcatgg tcagcctgtg cgaacagtta tcgaaactgg ggatctggac
    21241 acgtctgcac tacgtttacc cttatccgca tgtggacgac gtcatcccac tgatggcaga
    21301 aggcaaaatc ctgccgtatc tggacattcc gttgcagcac gccagcccgc gcattctcaa
    21361 actgatgaag cgtccgggtt ctgtagatcg ccaactcgcg cgcatcaaac agtggcgcga
    21421 aatctgcccg gaactgaccc tacgctcaac ctttattgtc ggcttccctg gcgagacaga
    21481 agaagatttc cagatgctac tcgacttcct gaaagaagcg cgtctggatc gcgttggctg
    21541 ctttaaatac agcccggttg aaggtgcaga cgccaatgcc ctgcctgacc aggttccgga
    21601 agaagtgaaa gaagaacgct ggaaccgttt catgcagttg cagcagcaga tttccgccga
    21661 gcgcctgcaa gagaaagtgg gccgtgaaat tctggtgatt atcgacgaag tggacgaaga
    21721 aggtgcgatt ggtcgcagca tggcagatgc accggaaatc gacggcgcgg tttatctcaa
    21781 tggtgaaacc aacgttaagc cgggtgatat cctgcgtgtg aaagtcgagc acgccgatga
    21841 gtacgatttg tggggtagcc gggtttaatt aatcttaagg cagatctaac aagaatattg
    21901 ttcttcgatc tgcctctgct acccttccct atgtcctacc cttacgcaca aaatcccctg
    21961 acgcataaat cgagaaaaat gaatcgtact cgaggcaact ctcacttttt cacaccaaat
    22021 taaagttttg atcgcaatta cttgaaataa gcgtaatggt tactatagta aacattacca
    22081 caaggatgga tgtatgggtg tgtataaggc aagacgattc agccaaagca cgaaaaaact
    22141 tggtattcac gataaagttc tcatggctgc cgctgaagaa gtgatgcagg gcatatggga
    22201 ggctgattta ggtagcggtg tcatcaaaaa acgtttaccc ctccaacaag gaaagagtgg
    22261 tggagccaga acaattatct tttttaaatc agcaaatcat gtgttttttt atgatggttg
    22321 gtccaagagt ggtctaagca gtaaaggaac caaagaaatc gaagatgatg aactggcggc
    22381 ctacaaaaaa atggcaaatg catttctggc ctttagcaat aagcaaatag aggatttgat
    22441 cgaaactggc tttttgattg aggtgaaaaa tgagcgataa attacaaatg atgctggaag
    22501 atgcaaagga actgaatgca ctgggcctga tgtctgatag tgaagttgat gccattgccg
    22561 tcatggtcaa agctcgtgaa gtcagcgccc gtatagccaa agtcaaatca atgacgggtg
    22621 aagagatcaa agccatccgc acccgatacg gcatgtcaca atccgtgctg gcacatacaa
    22681 tgggaatgtc aaaagaaagt gtctcgaaat gggaacgaaa tgaaataaaa ccgagcggcc
    22741 cggcactacg tattttgaat acattggcaa taaaaggacc ggaagttttt gcaaagtaaa
    22801 atgtttgcaa atgatttacc acattcatta gtaccccttg attaaggggt actaacacta
    22861 ttctaattct actgccagct aatctgcgcc ctgccgtttt tcttactctt atacagccgc
    22921 tcatccgcca gcattttcaa gcgttcgacg gtagtacatt catttgaatg cgctacaccg
    22981 atactggtgc ttaaattaat gcttcccacc tggaaatgaa cgatttcttt tagaatcgat
    23041 agcaactctt ccagcgttcc acccgtataa atcaccgcaa attcgtcacc gccgaagcgg
    23101 tagactttac ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac
    23161 acttcatcgc ccagcaaatg accgaaggtg tcgttgatgt ttttgaattt atcgacgtca
    23221 aacaggcaca aataaccacc tttttccggt agcgctttta aatcttcgtc aaaggatttt
    23281 ctgcccatcg ctttagtcag gtcgtccagg aacgccgagt tatacatact gtagagtttt
    23341 ttgacatcgg cagccgaaaa gaaaagcccg ccatctagta cgcgttcaaa atggttatta
    23401 ataatggctt ctttctccac aataccacga ttaatcactc tgcgtaccag atagaggcaa
    23461 ctggcacccg atatcactgc caccaatgcg gcaaagaaaa taatcaacgt acaaacccgc
    23521 aaataaacat cttttttata cgcaaatacg tacaatccgg tcggcaaata ggagaagtgc
    23581 tccatcgctt ccgaaacggt gaagatggcg tttttagcat tatattcatc accggtcacc
    23641 ggtttaccgt tcttaaacag agagaacatc aggttctcgc tatccatcgc cagcgatttt
    23701 gacagtaaag aaaaacgctc cagaccaagc aaaaatatca gactgcgatt gtgagttacc
    23761 ggatagaggt aatagacgaa agttttaccg tcaaaagaga taaaccctgt atttttgaca
    23821 tgtcgatact tcacaacctg cataatatct gcacaataat ttttctcgaa ctcctttacc
    23881 tgaatacatt cgtcactgga aatcagataa ggaccgacat cgtacaaata agagggtgaa
    23941 accgagtcgg cgtaagctgt gcgcaagcca accaacagct gttcctccgt ggggtttagc
    24001 gtgttgatga gatcaattgc cacgctacgc cgatattcag caatatttgc caccgcaaaa
    24061 gcagtatcaa taacggactg tttcaggcca tacacccgtt cctttaccat ttgagtgtag
    24121 atcccgcagg caactgctga aatcatgata aaaatcagca gactgactgt aagtacgaac
    24181 ttattgattc tggacatacg taaactactc ttttactaat tttccacttt tatcccaggc
    24241 ggagaatggc atgggtttct ggtacagata cccctgagcc tgaacacatc ctaatttgat
    24301 taattcgcgc gcaacatctt cgctctctac gccttccgca atcaccggaa tccggttata
    24361 gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt
    24421 aaattgcgag tccactttta tgctatctgg attaatttca taaaaacgtt tcaggtttga
    24481 caaaccggtg ccaaagtcat caatcgatat ccgtacccct tgtttacgca gttgttcaat
    24541 attgagataa aaatgttcat taattcgctc gatcacggtt tcagtgatct caacacagag
    24601 gctgttcgcc ttcagaccgt tttctttcat gatggtggta aaacgctcat gaaagtgtgg
    24661 ttcgtttaac tgccagggcg aaacattaat atgcagtaag aaaccgtcct tcagaaccgc
    24721 acgctcgcta aaatgcctga attcacccat tgccatttta ataatctgat aacccagctc
    24781 attgataaac ccgatatctt cagcaatggt aatgaacttt agcggtgaaa tgatcccccg
    24841 ctccgcagac acccaacggc acaacgcctc cgcgcccaga atggtgcggg tgttaatatc
    24901 aacgatcggc tgatagacca ggaatagttc gccaatttcc attgcatggc gaatattacg
    24961 cgcaatccag atattctctt ctatttcgtt gagtttcgcg tcatcacaga aaataaactc
    25021 tgagaacata tggtcacgaa tgctggaaat cgccagtttc agtttttctg cgtaactcca
    25081 ggcgttttcc ccctgcaaag gttcacaaat ggcagactta tggatgcgca gattgcggta
    25141 gccaaagctg ctgtcaaaat cgtttaccat cgctatttta cgggtaaatg tctgtacatt
    25201 ttcccggcac agaagtaaat agagatcggc attaaatgtc acgatatcgc agcattgcgc
    25261 atatttctcg gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc
    25321 aaacaccgct tccacggctt ccagaccaca cagtttgatg atccccacag ccagatactg
    25381 attattgttg gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga
    25441 gatcagtgag agcaatttat ttgaccgttc aatttcggca tctttcattt ccgcgatcga
    25501 ttttttgatg ctgataatct ctttcgcatc gccttcgcga attttcagag cctcgatttg
    25561 atccgttgag tcaggcaaaa acgccagcgt attaaccagc tctgtcattt cagaaaattg
    25621 ccgacggaac acacgagtgg tcattaaata aaccaccagc aatacgataa taatgaaagc
    25681 aaacggcaga taaaaaagtt tatctgatgc gttcaataaa ttaccgccag gataataaaa
    25741 atagatttcg aataatgcgt tgtttggcac cgctgatgtt ttttcgatat attc
//
LOCUS       DAYPHN010000062        25148 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000062 DAYPHN010000000
VERSION     DAYPHN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.062"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            6..353
                     /gene="grxD"
                     /locus_tag="ACG0BW_002376"
     CDS             6..353
                     /gene="grxD"
                     /locus_tag="ACG0BW_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFK7259327.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(404..5020)
                     /gene="lhr"
                     /locus_tag="ACG0BW_002377"
     CDS             complement(404..5020)
                     /gene="lhr"
                     /locus_tag="ACG0BW_002377"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFK7259328.1"
                     /translation="MADNPNLSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSGEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTSAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKDELLAPEIFQALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(5113..5760)
                     /gene="rnt"
                     /locus_tag="ACG0BW_002378"
     CDS             complement(5113..5760)
                     /gene="rnt"
                     /locus_tag="ACG0BW_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFK7259329.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5863..6270)
                     /gene="gloA"
                     /locus_tag="ACG0BW_002379"
     CDS             complement(5863..6270)
                     /gene="gloA"
                     /locus_tag="ACG0BW_002379"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFK7259330.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6351..7448)
                     /gene="nemA"
                     /locus_tag="ACG0BW_002380"
     CDS             complement(6351..7448)
                     /gene="nemA"
                     /locus_tag="ACG0BW_002380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFK7259331.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7485..8084)
                     /gene="nemR"
                     /locus_tag="ACG0BW_002381"
     CDS             complement(7485..8084)
                     /gene="nemR"
                     /locus_tag="ACG0BW_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFK7259332.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIVLLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            8188..8427
                     /gene="ydhL"
                     /locus_tag="ACG0BW_002382"
     CDS             8188..8427
                     /gene="ydhL"
                     /locus_tag="ACG0BW_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFK7259333.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8476..9372
                     /gene="ydhF"
                     /locus_tag="ACG0BW_002383"
     CDS             8476..9372
                     /gene="ydhF"
                     /locus_tag="ACG0BW_002383"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFK7259334.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTTREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9453..9974
                     /gene="sodC"
                     /locus_tag="ACG0BW_002384"
     CDS             9453..9974
                     /gene="sodC"
                     /locus_tag="ACG0BW_002384"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFK7259335.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9975..11987)
                     /gene="ydhK"
                     /locus_tag="ACG0BW_002385"
     CDS             complement(9975..11987)
                     /gene="ydhK"
                     /locus_tag="ACG0BW_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFK7259336.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSGARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(11987..12844)
                     /gene="ydhJ"
                     /locus_tag="ACG0BW_002386"
     CDS             complement(11987..12844)
                     /gene="ydhJ"
                     /locus_tag="ACG0BW_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFK7259337.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12847..13083)
                     /gene="ydhI"
                     /locus_tag="ACG0BW_002387"
     CDS             complement(12847..13083)
                     /gene="ydhI"
                     /locus_tag="ACG0BW_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFK7259338.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13284..13718
                     /gene="slyA"
                     /locus_tag="ACG0BW_002388"
     CDS             13284..13718
                     /gene="slyA"
                     /locus_tag="ACG0BW_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFK7259339.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(13765..14232)
                     /gene="slyB"
                     /locus_tag="ACG0BW_002389"
     CDS             complement(13765..14232)
                     /gene="slyB"
                     /locus_tag="ACG0BW_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFK7259340.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14506..15615
                     /gene="anmK"
                     /locus_tag="ACG0BW_002390"
     CDS             14506..15615
                     /gene="anmK"
                     /locus_tag="ACG0BW_002390"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFK7259341.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15713..16042
                     /gene="mliC"
                     /locus_tag="ACG0BW_002391"
     CDS             15713..16042
                     /gene="mliC"
                     /locus_tag="ACG0BW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFK7259342.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            16101..16757
                     /gene="pdxH"
                     /locus_tag="ACG0BW_002392"
     CDS             16101..16757
                     /gene="pdxH"
                     /locus_tag="ACG0BW_002392"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFK7259343.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16886..18160
                     /gene="tyrS"
                     /locus_tag="ACG0BW_002393"
     CDS             16886..18160
                     /gene="tyrS"
                     /locus_tag="ACG0BW_002393"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFK7259344.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18222..19082
                     /gene="pdxY"
                     /locus_tag="ACG0BW_002394"
     CDS             18222..19082
                     /gene="pdxY"
                     /locus_tag="ACG0BW_002394"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFK7259345.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(19126..19731)
                     /gene="gstA"
                     /locus_tag="ACG0BW_002395"
     CDS             complement(19126..19731)
                     /gene="gstA"
                     /locus_tag="ACG0BW_002395"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFK7259346.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNDALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19837..21339)
                     /gene="dtpA"
                     /locus_tag="ACG0BW_002396"
     CDS             complement(19837..21339)
                     /gene="dtpA"
                     /locus_tag="ACG0BW_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFK7259347.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21950..22585)
                     /gene="nth"
                     /locus_tag="ACG0BW_002397"
     CDS             complement(21950..22585)
                     /gene="nth"
                     /locus_tag="ACG0BW_002397"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFK7259348.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPTAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVNI"
     gene            complement(22585..23280)
                     /gene="rsxE"
                     /locus_tag="ACG0BW_002398"
     CDS             complement(22585..23280)
                     /gene="rsxE"
                     /locus_tag="ACG0BW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFK7259349.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23284..23904)
                     /gene="rsxG"
                     /locus_tag="ACG0BW_002399"
     CDS             complement(23284..23904)
                     /gene="rsxG"
                     /locus_tag="ACG0BW_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFK7259350.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(23908..24966)
                     /gene="rsxD"
                     /locus_tag="ACG0BW_002400"
     CDS             complement(23908..24966)
                     /gene="rsxD"
                     /locus_tag="ACG0BW_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFK7259351.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24967..>25148)
                     /locus_tag="ACG0BW_002401"
     CDS             complement(24967..>25148)
                     /locus_tag="ACG0BW_002401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259352.1"
                     /translation="PRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIAR
                     VQAKKAAQQKVVNED"
BASE COUNT         6226 a         6497 c         6583 g         5842 t
ORIGIN      
        1 agacaatgag caccactatc gaaaaaatcc aacgccagat tgctgaaaac ccgatcctgc
       61 tgtacatgaa aggttcaccg aaactgccga gctgcggttt ctctgcccag gcagtccagg
      121 cgcttgccgc atgtggcgaa cgttttgcct atgttgatat tctgcagaat ccggacattc
      181 gtgcggaact gccgaaatat gctaactggc cgaccttccc gcaactgtgg gttgacggcg
      241 agctggtcgg cggttgtgat atcgtgatcg aaatgtatca gcgtggcgaa ctgcagcagc
      301 tgatcaaaga aactgccgct aaatacaagt ctgaagagcc ggacgcggaa taatccgtcc
      361 tgcagtactg acggatgcga acgggcatcc gtcagttctt tatctatccc caatccagcc
      421 cttgtggcga acttgaaaat cccgcctcac gcagcagcgt aaacatcggc gtttgccgga
      481 ccggtagatc attcacttct gttagcgtaa agcgcagccg tggttcgcga cgcagtgcgg
      541 tggtcagcgc ctggaaaatc tctggggcga gtaattcatc tttttcctgc cagaccagca
      601 tttttttgcc gccttgcgcc agatagagta acaatttgcc gccagaaacg accaccagcg
      661 cacccgcccg acgcgttgga accagcgttg ccggatgtgc aggccagggc agaagatttc
      721 cccacacatt agccggatcg ttggcggaga gcgccactgg tgtatagtgg cgcgtttgcg
      781 tggcttgtgt cgccagatcg cgcaatcggt caatagtcag acgttcagcg aattgcgcac
      841 cccccagacc ttctacaaaa cgacctcgca taattcgccc ggagtcttcc atacttcgac
      901 aaagcgtttg catcgatgga aacccgccag ggatattttc ggctatcacc gcctgacgac
      961 tgatgatgcc gtagcggtcg agcatatttt ccgccagcgc cagcatcctt tcggtatcgt
     1021 ttagtggctc cacctgcaat aacgaccagc gtccagcaag atttggtgtg ttgtaagata
     1081 cccgcggcga gacgggttgt gcatagacag gacgcccacg acgagcccgg tgactgcggc
     1141 gagttgaggt gcgtgcgttg gaactgctgc gggtgagagc gcgtaacggt gcccaaatat
     1201 cgctggtgat gacgccttgc cagaccagcg cccataacgc ctcttgcagt gccgagggat
     1261 caaccgattc gccgatttta tcgcgtatcc gttggctgat ttgttgtgca aaccaggctc
     1321 ctccgtcagc cagaacagcg attatcgctt gctgcagcgc cgaacgattc gcctgatccg
     1381 cttcggcgga agtgaacgat tctgcggcat attcctgtag atgcagtgcc accagaccgt
     1441 catcttcacc cagctttttt tgccccgacc agataaccgc accggttgcc agtaattcat
     1501 cgagcatttc tggcgaatag tcgcgtacgc gggcaggcag gatctggctt tcccagagtg
     1561 aggcgggtaa accgactccg gcaagctgtt cgatcacccg catgacgcca tccacgccct
     1621 cataaacgcc tggtgatgtt gaggcaaaga gtgccgggct accatcggtg gcgggtaata
     1681 cgccctgacg ttccagcagc aatcgcgcat aggtcgtggc cgcaacggga cgtgtcgctt
     1741 ctctggcggc ttgcagcgag cgcaaacgca gacgacgaaa tacttcatcg ctcacccaga
     1801 tgtcttgttg cagattcatc accagcccct gttcgcgcag ttgctgaagt tgctcttcga
     1861 caatggcaat accgagacta aactcatgag ccagttgttc agacgtgacc agagcatgag
     1921 cccggagata gcgcagaaag aggtcgcgaa gcgggtaact gactcggtgc aaataaatct
     1981 ctggcagtga ctctggtagt cgtacgccga gggcatcgcg cagcctggcg gcatcatcca
     2041 tacaggcaag acgctcctgc ccgctaatca tcgtcgggaa gattcgtttt actgcaagaa
     2101 gattttccag atacgacgca atctctccac tgctgcctgt atgccgttgc gccaggtctt
     2161 caacggtcat tggccccagt tcgcgcagca ggtcgaacaa tccttcttca cctttcgctc
     2221 ttctgccagg agccagtcgt tgcaactctt cttccacctg gcgaatgacc tgtgggtcga
     2281 gtaattcccc cggatcgacc tgtccgagta gattgcgcag taactcgctg tccagcgaca
     2341 gtacggatgc ccggcgctct gccagcgggg cgtcgctctg gtacataaat tccgcgacat
     2401 agccgaacaa taaactggca gcgaaaggcg aaggcgtagt ggtcgttaca tcggatattt
     2461 gaatttcgcc accgttcagg cgacgcatca aacgttccag cgcgggaaga tcataaacat
     2521 cttgcagaca ttcgcgtagg gtttcgagaa tgaccggaaa atctggatat ccctgagcga
     2581 tttccagcaa ctgactggcg cgcagccgtt gttgccagag cggggtgcga tggcccggcg
     2641 tgcggcccgg cattaataat gcccgcgcgg cgcattcgcg aaaacgggcg gcgaaaagtg
     2701 ccgagctgcc taccgcctcg cggacaattt gcagcaactt ttctggttca aacaaaaaaa
     2761 tcgcggcatc gggcaattta ccatcggtgt caggaatgcg cgcaacaatg ccgtcatcac
     2821 tggcaaccac agacgcgtca gcgccccata gtgcatgtat tcgcccggca atcgccagcg
     2881 cccagggttc atgcacccgt cttccatagg gagagtgcaa aataatacgc cagtcaccaa
     2941 tttcatcacg gcaccgttcg aggaccagat gacgactccc cggaacgatg cccgtcgcgt
     3001 tgcgctgctc gtcaatcaac ccctgaatat tggcgatcgt attttcttct gccaaccacg
     3061 ggggaatagt cccggaaaag aacgcgccat ccgccagtaa atggagaaaa tcgccgatca
     3121 tctcgcctaa ttctgccgga cgtccgttac cttcaccacg ccagaagggg agccgggcag
     3181 aacgacccgg agcaggagtc acaatcacct gatcgcgggt gatttgctgg atccgccatg
     3241 aggtagcgcc gagagtgata atgtcgttca cccgcgactc atataccatc tcctcatcca
     3301 gttcacccac ccgccgcgaa ccggcctgtt cttcaccttc gggtaataac acgctataca
     3361 tgccacgatc cggaatggtg ccaccgctgg taaccgccaa cagctgagcg ccaggtcggg
     3421 cggtcaatat cccggtctcc cggttccaga ccagtttggg gcgaaaagca gaaaaatcgc
     3481 cagagggata gcgcccggaa agcatatcca gcgtggcgtc aaaaacacgt cttggcagat
     3541 ctttccacgg tgcggcacgg cgtacgcggg agtaccattc gtctacctgt aatgcatcca
     3601 tcgccgcggc ggcaacggtt tgctgcgcaa ggacgtcgag tggattatgc ggtggtgtca
     3661 ggttttccag cctgcctgcg aacatacact ctacaatgac tgcggaatcg actaagtcac
     3721 gccgggttcg ggggaaaaac agccctttgg agatacctcc tacctggtgt ccggcacgtc
     3781 caatgcgttg taacccactg gcaacagaaa gcggcgttgc cacctgaatc accagatcca
     3841 ccgcgcccat atcaatcccc agttcgagac tggaggttgc gaccacacag cgtaattcac
     3901 ccgatttcag cgcctgttcg gtgattgctc gttgttcttt agagacggag ccgtggtgtg
     3961 agcgggcaat aaaaatgtcg ctactttgta cacggttaga ggttgcgccg gaggtcgact
     4021 cgaaatgggc cgcatcaacg gcgatagacg gggaacgctg taagcgtgcg gcgtaaagct
     4081 catttaatcg tgccgtcagt ttttccgcca gtccccgcga attagtaaag acaatggtcg
     4141 agcgatggcg caacacttca tcaaggatac ccgtttcaat atatggccag atggagcctt
     4201 cccggccagc atggctgtct tcgccagtgc cgctggcgac cgatgagaca tcatccatat
     4261 tggcgaccgg tacgacaatc cgtatctgcg gatggcgcat tgcgggcggg ttgactaccg
     4321 taaccgggcg atcgccgcca agaaatgctg ccacatcgct ggctgaacgc acagtggcag
     4381 aaaggccaat tcgctgtgct gaggtgtgga gcagcgcatc gagccgctcc agacttaacg
     4441 ccagatgcgc accacgttta ctgcctgcta ccgcgtggac ttcatcaata attaccgttt
     4501 cgacgccgcg tagcgtttcg cgcgcgcggg aggtcagcat cagatagagt gattcgggtg
     4561 tggtgatcag aatatccggc ggattacggg tgagtttgct gcgttcctgt gcaggcgtat
     4621 cgccagtacg gatccccacg cgaagattga cttccgtttc gccgcgccgc cgccgttcat
     4681 cggcaatacc ctgtaacggg atctgcaagt tgcgctgaac gtcggtgcca agggctttta
     4741 tcggtgaaat atagaggatg cgtgaggttt tacgtttatg cgcctcgcgg gtatcttcgc
     4801 cgccttcgcg gaagagctga tcgagggcgt agagaaatgc tgccagcgtt ttcccggagc
     4861 cagtcggtgc aatcaccagc gcatgttcgc ctcgcgccgc cacatgccag gtttgcgact
     4921 ggacagcggt cggctgttta aaggcgcgaa gaaaccagtc gcgggttgcc ggtgaaaaca
     4981 cgtccggcag gggcgatgaa aggtttggat tatctgccat taacgctatt ccccgtgaat
     5041 atcatgcatg atgcgtggaa tagcctcact atagatgcct gaatcattgc atgtcatcag
     5101 gcatcgactc gattacactt cttcggcggc aggtagcggc caacctccca gacgtttcca
     5161 gcggttgacg atttcacaaa acagcacagc agtgcgttcg gtgtcgtaca gcgcggagtg
     5221 cgcctgggtg ctgtcgaagt ccatgccagc ggtctggcaa gcctttgaca atacggtttg
     5281 tccgagtgcc agcccggcca gtgcagcagt gtcaaaagtg gcgaaagggt ggaacgggtt
     5341 acgtttcagt gaggcgcgtt ctgcggcggc catcataaag ctgtgatcga aattggcgtt
     5401 gtgcgccacc ataatggcgc ggttacaacc gctcgcttta atacctttac gcacaacttt
     5461 aaaaatttcg tgcagcgcct cgtattcgct gaccgcgccg cgatcgggat cgttcgggtc
     5521 aatgccgttg aaggcgaggg cctctggctg caaatttgcg ccgacaaatg gttcgacgtg
     5581 gaaatgtaat gtggtgtccg gcatcagcca gccttgttca tccattttca gggtgatggc
     5641 ggcaatctca agcagcgcat cggttttggc gttaaatccg gctgtttcaa catcgatcac
     5701 aacaggataa aaaccacgaa aacggtcgca cagaccggta agttgagcgt tatcggacat
     5761 ctgattctct taatacggat aaattgcagc gcgcattatg acaaatatta cttgtcgatg
     5821 cagtaaagat gcgggcgcga tgagttcacg cccggcagga gattagttgc ccagaccgcg
     5881 accggcgtct ttctcttcga ttaattcaat tttgtaaccg tccggatctt ccacaaacgc
     5941 gataaccgta gtaccgcctt ttaccggacc cgcttcacgg gtgacgttac ccccgttttg
     6001 acggattttt tcgcacgctt cagcggcgtt atcaacgcta agcgcgatgt gaccataagc
     6061 agtgccgagt tcgtatttat ccacgcccca gttgtaggtc agttcaatca ccgcttcttc
     6121 ggtttccggg ccgtagccaa caaacgccag tgagtatttg tattccgggt tttcgctggt
     6181 acgcagcagt ttcatgccca gcactttggt ataaaaatcg atggagcgtt gcaaatcgcc
     6241 aacgcgcagc atggtatgaa gaagacgcat ctttatatcc tcaaaatggc taacagattc
     6301 aaaatgttgt tttagtatag cggcggcttt acgccgctcg caatgttgga ttacagcgtc
     6361 gggtaatccg tgtagccttc cgcaccgcct ccatagaaac tttcggcacg ctgtgggtta
     6421 agctcagctt tgcgttgcaa gcgggcgacc agatccgggt tcgcaatcca gtcacgacca
     6481 aatgccaccg catcaattaa ccctttgccg atcagcgttt cggctttttc taccgtgtat
     6541 gcacctgcgc cgataatcgg accgtggaaa cgggcgcgta ctttttcgcg gaacgcatca
     6601 gtatacggtt caccccccgc ccaatctggt tctgacatat gcagataagc aatgccgcgt
     6661 ttacccagtt gttcaatcag atacagtgca tcggcttctt cattcgggcc gttatctgtg
     6721 ttctggaaag taccgattgg cgaaacgcga atgccaatgc gatcggcacc ccattcttca
     6781 atccctgcat cgaccacttc cagcaccaga cgtgcgcgat tttccacgct gccgccgtac
     6841 tgatcggtac gatggtttga agaaggagaa aggaactgat gcagcaaata accatgagca
     6901 gagtggagct ctaccagatc aaaaccggct tcacgcgcgt tagcaatggc ctgacggaaa
     6961 tcattgacga tacccggaat ctcttccagt tcaagcgcac gcggcatgga tgtttcaaca
     7021 cggatcgcct gaccattttc atcgcgcaga gaagtacgtg ttcccgcgct aagtgcggaa
     7081 ggcgctaccg gtgcctgacc gccaggttgc aggctggcgt gagaaatgcg tccggtgtgc
     7141 cacagctgta cggccatatg accattttca gcatgaacgc cagcggtaat ttttttccat
     7201 gcggcaattt gttccggact atggatgcca ggtgcaccag catacccttt tgcctgggca
     7261 gaaatttgcg tggcttcgct aataatcaaa ccggcactgg cacgttggcg atagtattcc
     7321 gccatcaacg gggtagggat gtcacccggt tcaatactgc gcagacgcgt cagcggtgcc
     7381 ataaaaatac ggtttgccgc cgtgatcgcg cccactttca gtggggaata cagtttttca
     7441 gatgacataa aggctcccgg tcgtctggtg ataaaaaatg cctgctaaac ggcaggcgtc
     7501 gcaataatgg tttttacatg tgccagcgcg ttttccagtg gctcggaatt gcgcgaaatt
     7561 ttggcctgca gattcgcacc cagccatagt gcgtaaagca cttgtgcctg ttgcagcggt
     7621 tcgccacaaa aggttaaaca atggctatca cggccatttt ccagcgcctg cgagagcaga
     7681 acgatcacgc cgcgagcgcc tttatccatc gcgctacgca tatcttctga cagatcgcac
     7741 acttcggcag agagttttac tgtcaggcaa ccactgatgg ttccatgttg gcaaaactgg
     7801 ttcagtgttt gctggtaata agccagtatg cggtcgcggt agttaccttc gccggattgc
     7861 agcaactcag tcagtcgctg gtgatatgcg gcgtaatggc gctcaagcat ggcaacgcca
     7921 aacgcttctt tagagcgaaa gtagtgatag aaggaccctt tcggcacttc agcggttttt
     7981 agtaattcgc ttagccccat cccggtgaat ccacgttgca ggcaaagttg ctcgcccgtt
     8041 gccaggagat gttcgcgagt atcatgttcg gtgtgtttgt tcatgcgttg gagtgtagta
     8101 gaccagtcgg tctagtgcaa gcaaagttgc tactttgggc attaacagct tcaataatgg
     8161 taataatctt attttgagga gggcgaagtg gcggagcaat tagagttctt tcccgtccag
     8221 agcccgtgcc ggggaatttg ccagtctgac gaacgcgggt tttgtcgcgg ctgttttcgt
     8281 agtcgggatg agcgttttaa ctggaataaa atgagcgatg gcgaaaaaca agaggtgctg
     8341 cgcctttgtc ggcagcgatt aatgcgcaaa ttacgcgcaa ataagccagc gtcatcagac
     8401 gaaccggagc aaccatcact cttttgagcc taatcttgcg tatactcctc tcatatctga
     8461 taagaggaag cgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc
     8521 cgttttgtga tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc
     8581 agttttattg aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat
     8641 ggtggctatc agtgcgaagc ggcgtttggc gaggcgctga aacttgcacc tcatctgcgt
     8701 gaacggatgg aaatcgtcag taaatgcggt atcgcgacga ccacgcgtga agaaaacgtc
     8761 attggtcatt acatcactga ccgcgatcac atcattaaga gcgccgaaca gtcgctaatt
     8821 aatctcgcga ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat
     8881 gccgatgaag tggcggacgc gtttaaacat ctgcatcaga gcggcaaagt gcgccatttt
     8941 ggcgtatcga actttacgcc tgcgcaattt gcgcttttgc aatcacgcct gccgtttacc
     9001 cttgccacta atcaggtgga aatatccccg gtacatcagc cgttactgct ggatggtacg
     9061 ctcgaccaac tgcaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt
     9121 cgtctgttta atgatgatta tttccagccg ctgcgtgatg aactggctgt ggtggcagag
     9181 gagttaaacg cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccgtcg
     9241 cagccgctgc caattatcgg ctcaggtaaa attgagcgcg tacgggcagc tgtcgaagca
     9301 gaaacactga aaatgacccg tcaacaatgg tttcgtatcc gtaaagcggc actgggttac
     9361 gacgtaccgt aagcactttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac
     9421 ttattcagga atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gccattctgg
     9481 cgctggttgt tgcgaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca
     9541 cgtcgcaagg ggttgggcag tcaattggta gcgtcaccat tactgaaacc gataaaggtc
     9601 tggagttttc gcccgacctg aaagcattac cccccggtga acatggcttc catattcatg
     9661 ccaaaggaag ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag
     9721 gcgggcatct tgatccacaa aataccggta aacatgaagg cccagaaggt gccgggcatt
     9781 taggcgatct gcctgcactg gtcgtcaata atgacggcaa agctaccgat gccgtcatcg
     9841 cgcctcgtct gaaatcactg gatgaaatca aagacaaagc gctgatggtc cacgttggcg
     9901 gcgataatat gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg
     9961 gtgtgattaa gtaattaaga ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa
    10021 agcgagcagt acagccgcca gacaattgcc gccagctcgc gggcggcagg ttgatgatgg
    10081 cgggcaaggc tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc
    10141 tgaacgccac gctcactcat cactccgcgc aacagtgaaa tacagttatc ccgtactcgt
    10201 gataacggat cggaacgcga ttcccagtcg cgtaattgcc agacaacatg agaacagttc
    10261 agcagcacta caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc
    10321 aactgactga cgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg
    10381 cggcttagct gatcgacaaa atcccggcgc agcgcgcgaa tatgacgacg gcttttacga
    10441 gcacccgacc ctggacgcaa aatggcgaac gctaaccacg ccagcgcaac gccaacgatt
    10501 tttgccagat tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg
    10561 ataaacgaac ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc
    10621 aatttaagaa gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga
    10681 tcgctaatct ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc
    10741 agagtgcgca tcagtagtga caacgactta aacggtgctg cgacggcgga gtagagtacg
    10801 cagctaattg ctgccagcgt taatgcattg gcaccggcat cccattgcga agcaatactc
    10861 catgcgccaa tcatcatcaa cgtacaaaat gtacgcagtc cgctccacat agcttcggcg
    10921 ttatcggtat gacgagccag gccggatgtc cgtgggagtc tggtatgatc atccacaccg
    10981 ctttgcagac gatgtaattc ctgactactt tgcagataaa ggcggcaaaa ataacgtagt
    11041 cgctgccaga aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag cggggcgata
    11101 atacgtgcga cggtgtaaac atctgtttgc gaactggcga gcgccgtcag cagctgttcg
    11161 agaatttctc gtgtggcaca tggcggtgag ggccagttga gcaacatacg tcgcaggctg
    11221 gagatgacac tggtcatacg taattgctgg tggagcagcg cattaaggcg tgcgttttgc
    11281 tggcgaaaac gatagtggct ccagaaagcc tggatacgga gcaaattcat ggtcagtatc
    11341 tgtccaatca ccccctcatg tgctgcacga atggcatcgg ttgtttcagg ctgccagagt
    11401 aaactggcat gttccagtaa tcgggcgtgc atgtttttca atgcggttaa aagggcagta
    11461 gcatcggaac tgctcggcag gatcatcatc atcatgccgc cgcacaaaat accgacaatc
    11521 acctcgcaaa cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc
    11581 atcggaaagg cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacatta
    11641 ttcgtgaagt gcgcacaggc ccaggtacaa aagccaagcc acgccgccat gctcaaaaga
    11701 aaaaaccacg gctcattgag cgtatgtccg gcaagaagca gtgccgcaat ggctccgagc
    11761 aaactgccag cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta
    11821 accacggcag ccgaggtcat cgcccaatag ggttcatcca gatttaaata ataggcaacc
    11881 gtcagcgcca gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg
    11941 gccctgaacc agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg
    12001 caatgctaca ggtggtgccc gacaccagcg tgacatcgcc tgggacttta tccagcgcaa
    12061 agcggacggg aacgcgttgg gcaagtcgaa cccaggggac gttaggtttc acatccggaa
    12121 taaggcttga gtcactttca acgctctgat cataaatcgc ccgaccgata ctggaaacgt
    12181 gaccctgtaa cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga
    12241 tatggcgcaa cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa
    12301 gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca
    12361 cccatccact caccggggcg cgaatttccg tttgcgccag ttgccattgt gcctgtttca
    12421 gcgtcgcctg tgcggcatca acactggcct gcatcgcttt aacattgagg ttagcggtat
    12481 cgagttcttc ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat
    12541 tggcttttgc gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga
    12601 tctgaaaggg cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agttggttgt
    12661 cttttatatt cagctcaaca atacgtcccg acacctgagg ggtgatagaa acctgttcag
    12721 cgcgtatttt cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc
    12781 cggcaagaac cgcaactacc gctacaatga ttgttgagaa atacttaatt gttttaattg
    12841 acatagttac cacgcaatca gtattgccag ggcaaggcaa acgcaaatcg caaacagcga
    12901 taaatccatt aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg
    12961 gtgtacgaca agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaagta
    13021 gacggacgca ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt
    13081 cacaaagtag attcctttac gaccgaatat attgcgttag attaataaat attctttaag
    13141 tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat
    13201 acatttgttt tagcaataca attgctgcac actattctaa aagccgcata atatcttagc
    13261 aagctaatta taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg
    13321 tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc
    13381 attgggttac gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg
    13441 caaaagcgat tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa
    13501 aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga
    13561 cggaaaaggc agagccgctg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg
    13621 aaattttaca tggcatctcc gcagaggaac tggaacaact gattaagctc atcgcaaaac
    13681 ttgagcataa tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag
    13741 taacctgacc acacgcaaac ttatttagcg cggggaaacg gtcacctgac tgccattgct
    13801 ggccagtacg acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat
    13861 gatggtatta ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc
    13921 actctgtacg ccctgaccgg ctacgccacc tgcaacagcg cctgctgcag tagccagaga
    13981 acgcccggtt ccgccaccaa cagtattccc aaggaaacca ccaagaacag caccgccaat
    14041 tgcgccgata acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat
    14101 ggtgccatag ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga
    14161 cagggtgtcg ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac
    14221 gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg
    14281 gtcaaagtat atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc
    14341 ataatgaaag taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa
    14401 gataatgttt tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt
    14461 tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc
    14521 tttattggcg ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaatt
    14581 gatgaacacc gggtcgcaca gctggcaagt ttgagctggc cgatcccggt gtctctgaaa
    14641 caggctgtac tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt
    14701 gatactcaac tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac
    14761 ctgcaggcga gagatatcgt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg
    14821 acgggcgtgg caccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc
    14881 ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca
    14941 ccgctggtac ctgcgttcca tcatgcgctg ctggctcacc caaccgagcg acgaatggtg
    15001 ctcaatattg gtggcatcgc caatctgtca ctgctcattc ctgggcagcc ggtagggggc
    15061 tacgataccg gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggcaggtaaa
    15121 ccttacgata aagatgccga gtgggcacgg gcgggtaaag tcattctccc actgctgcaa
    15181 aatatgctta gcgacccgta tttctcgcaa cctgcaccga aaagtaccgg acgcgaatac
    15241 tttaactacg gctggctgga gcgccatttg cgccattttc cgggtgttga tccccgagat
    15301 gtacaggcga cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc
    15361 ggtggctgcg aacgattgat ggtatgtggt ggaggaagtc gtaatccgct actcatggcg
    15421 cgtctggcgg cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt
    15481 ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta
    15541 ccaggaaatc tgccttccgt cactggcgca agccaggaga cggtactcgg ggctattttc
    15601 cccgctaacc cgtgatatca gagttaactg aatttttctt accgcgtctg ccgttacact
    15661 tggaacgtta gggagggcgt attgccctcc agaccaggaa agtcttcggg atatgtctat
    15721 gaaaaaactg ttactgatct gtttgccggt ccttctcacc ggctgtagcg cgttcaatca
    15781 actggttgag cgcatgcaaa ccgatacgct ggaataccag tgtgatgaaa aaccgttgac
    15841 ggtcaaactg aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca
    15901 tctcaaacag ggcatttctg cgtctggtgc acgttacacc gacgggatct atgttttctg
    15961 gtcgaaaggc gaggaagcga ctgtctataa acgcgaccgc atcgtcttga ataactgtca
    16021 gttacaaaat ccacagcgtt gagatttttc caggggcggc gcacaatagc gtcacccact
    16081 gacaatccgt aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc
    16141 gccgtgaata caccaaaggc ggattacgcc gccgcgatct tcccgccgat ccattaaccc
    16201 tttttgaacg ctggctctct caggcgtgtg aagccaaact ggcggaccct accgcgatgg
    16261 tggtcgctac cgtggatgaa catggtcagc cttatcagcg catcgtttta ctcaaacatt
    16321 acgacgaaaa aggcatggtg ttttacacca acctcggcag ccgtaaagca catcaaatcg
    16381 aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgagcgc caggtgatgg
    16441 tgatcggtaa agcggaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc
    16501 cgcgtgatag ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg
    16561 gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat
    16621 tgccgagttt ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg
    16681 gtgagcatcg cctgcatgac cgctttttgt accagcgtga aaatgatgcg tggaagattg
    16741 atcgtcttgc cccctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc
    16801 tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc
    16861 agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga
    16921 gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg
    16981 cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct
    17041 tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt
    17101 aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgctgagc gtaagctgaa
    17161 caccgaagaa actgttcagg aatgggtgga caaaatccgc aagcaggttg ccccgttcct
    17221 cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa
    17281 tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat
    17341 caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga
    17401 gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt
    17461 ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac
    17521 ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ccaaagcaga
    17581 cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag
    17641 cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct
    17701 gaagttcttc acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa
    17761 cagcggtaaa gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca
    17821 cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcctt
    17881 gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga
    17941 gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg
    18001 tggtcaggcg cgtaaaacca tcgcctccaa tgccatcacc attaacggtg aaaaacagtc
    18061 cgatcctgaa tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg
    18121 tcgcggcaaa aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg
    18181 agtgagaaat cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta
    18241 tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt ccgatgcgcc
    18301 gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac actcaatacg
    18361 gcaaatggac tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg
    18421 ccgccattga taaattacac acctgtgatg ccgtattaag tggctatctg ggttcggcgg
    18481 agcaggggga acatatcctc ggtatcgttc gtcaggtgaa agcagcgaat ccgcaggcga
    18541 aatatttttg cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg
    18601 tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg
    18661 ttgagctgga gatactctgt gagcatccgg taaataacgt cgaagaagcg gttctggcag
    18721 cgcgtgaact cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgggctg
    18781 gctacagccg tgaccgtttt gaaatgctgc tggttaccgc cgatgaagcc tggcatatca
    18841 gccgtccgct ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg
    18901 gtttactgct ggtgaaactg cttcaggggg caacgctgca ggaggccctg gaacatgtga
    18961 ccgctgcagt atacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag
    19021 tggttgctgc tcaggatcgt attgccaaac cagaacatta tttcagcgca acaaagctct
    19081 gaaatatctc atttcggccc acaacgacct gtgggccgat ggctattact ttaagccttc
    19141 cgctgacagc gcgtcttgta cttccggacg ttcagccata cgttgcataa atgccgcaat
    19201 gtgctctaac ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag
    19261 ataggcatca gcaattgtaa atcgttgccc gcagatccag tgctcatcct tcagagcatc
    19321 gttcacatat tgcagtttct tctccagctg cgcgcgaact gttggtttgt actcttccgg
    19381 tgtatcaggg cgaaacagag gtgtgaagcc tttatgcagc tcggtggcga tgtaattcag
    19441 ccattcgatg gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg
    19501 gacgctgtcg gcaagatact gcataattgc tacgccttcc gtcaacaaag taccatcgtc
    19561 cagcagcaat gcaggcacct gtcccttagg gttaacggca aagtaatcgt caccgttttc
    19621 gagacgtttt ttcattaaat ccacactgac gagcgtaaaa tcctttccgc tctcacgcag
    19681 ggtgatatgg gaagcgagag agcaggcgcc cggtttgtag aacaatttca tcggtaactc
    19741 cttttgtctg tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg
    19801 aaaagttagc ggcttgtaaa agagtttccc tgaattttac gctacagcag ctttcgccgc
    19861 tttgtctgca gcgtcatcct gcgtcatgcg gtgcagtttc ggcgcagtca gcagcatcag
    19921 tactgcaata acggcagtag cgacaccaat ctgcaagaat acgcgaccat agacttccag
    19981 tgacatcagt gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc
    20041 aatcaggttt gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg
    20101 ctgcggaacg agttgtgcaa ccattgccag acccagacca gagatcataa gttccccgat
    20161 actctgcagg ccatagcttg cgaccagcca gcttacagac acgatacctg cgtcagacgc
    20221 gaattttgca cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc
    20281 aaacttggtt ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg
    20341 actaccgatg atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag
    20401 acccagaatg gagtgctcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg
    20461 gctgtacagc acgaagaaga tgattgcttc gagcatcagg atgaaggcaa cgatcatttt
    20521 acgacgcgca gcacctttca tcgcgaaagc ttctttaccg aagataacca caataccgaa
    20581 tgcaacaacg cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat
    20641 agcgatcagt gccacaacac caataatggt cagcagcagg ttacggtagt tgataggctc
    20701 gaagtctggt tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat
    20761 agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc
    20821 cagccacggg gtggcgatca tagagaagaa agagccgatg ttgacggaca tgtagtacat
    20881 ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga
    20941 cgggttagct ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacgat
    21001 accggcgtcg tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc
    21061 aagcataatt acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac
    21121 cagaccataa accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat
    21181 acccagttgt ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg
    21241 ttcccataac tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa
    21301 actgacgctt tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc
    21361 ccatattaac gggggtgctt tctcgcgtta tgcctaaaaa acatacgcgc aattgttata
    21421 gtgggagggg aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta
    21481 ataatctgtt acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc
    21541 gttaaatttt tcaaaaaacg taacggcaga ttttttcact accctgagag ggggatgatg
    21601 gcttaacggc gatatgttct gctatttagc gtttttccta gtgagatagt ccagtttctg
    21661 aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt gcgtgtgtaa atttaaatga
    21721 ttttaaccta attcttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcacgaa
    21781 tgaatagaaa atttcgctat taaaaaaaca attaacctgc atttttggtt atccgcaaac
    21841 ggattacctg atgataacgc taagcaatgg catcattgac gttaaaaaga tgttttaaaa
    21901 gaggataaag aaaggttatg aatggggtaa tcggtgttac cccttttctt cagatgttaa
    21961 ctttctcttt gtattcacaa agatcttcaa taatacaaga gccacagcgg ggcttgcggg
    22021 caatacaggt atatcgcccg tggaggatca accaatggtg gcagtcgact ttaaactctg
    22081 ccggaaccac tttcagtagc ttttcttcta cctgttcgac atttttcccc ggcgcaaatt
    22141 gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg
    22201 cagtgtttaa tacgacgttg gctgttttac gacctacacc aggcagggct tcaagcgcag
    22261 cacgatcttc cggaacctcg ccattatgct gttccagtaa gatacggcag gttttgatga
    22321 tattttctgc tttgctgtta taaaggccaa tagttttgat ataggttttc accccttcaa
    22381 cgcccagttc aagcatcgct gtaggcgtat tagccaccgg gtagagtttc gccgtcgcct
    22441 tattaacact gacatcggtc gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag
    22501 gcgaactgaa attaagctcg gtggtgggat gaggattgtt ctcacgcagg cgagtgagga
    22561 tctccaggcg ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg
    22621 ttcggcagct gcttcagcac ggcgcttttt cattctttca tcaatcaggt atttccctgc
    22681 cagcatcagt cccaggccaa taaatgcacc tggtggcagc atcgccagca ggaaagggga
    22741 gtcggtgtgg aaaatttcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc
    22801 accgtcaaac aacgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc
    22861 gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt
    22921 ggcggcgaag gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa
    22981 aatccctaat gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct
    23041 gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg
    23101 acgcagcgtc gaaatggtca agttggtcag cgtcagtacc agcgtagtcg caagtcctaa
    23161 acccagagcg ttagtggcag tggacgtgac cgccaatagc ggacagaggc cgagcagctg
    23221 gactagcgca gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac
    23281 gctttattct ccacaggcag gaagttgaga aagttgtgac ggtaacgtct gagcgtacaa
    23341 tccggcgcgt tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cggtgaactg
    23401 gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac cactgatttt
    23461 tttacccgca aaatgggtga tccagtcaga aaggcgcagt tcgattttat cgccaagccc
    23521 tggcgtttcg tggtgctctg tcacgcgcgt gcctagtacc gtgccgttaa aatcggctcc
    23581 caccagcagc tgaattgcac cagaatagcc atctggcgcg gttgcttcca gaacggcggc
    23641 tacaggtttg tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg
    23701 cgcagtgacc agatagcaac tctgtgccag cgcattgtta tagcgttcgg ctggcagcac
    23761 ctgatcaaat aacgcctttt gttgcagact ggcctgttca gcaattgtcg ttttggtcat
    23821 ctggttgatg gccgcagtta accctgtaga acccgctgca aatagtgcca gtgtaatgcc
    23881 gtgttttcgg atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg
    23941 cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc
    24001 gtcaggatag ccgccgaaac tgcggatcaa ccagactaat aaacccgcca gcgcgccgaa
    24061 aataagacga ccacgattgg tcgtagaagc agtaaccggg tcagtcaaaa taaagaatgc
    24121 gccgagcata gtcgcaccag acagcagatg aatttgcggt gatgccagtg tttctggtga
    24181 gaacaaccag cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg
    24241 ccagcgaatc gctttctgcc atagcaacca cacgccgcca gccagccagg cgagatttac
    24301 ccattgccaa ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctgttc
    24361 aaccgaatga ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg cctgactaat
    24421 gccatcaata cctaagcgta gcgtgttcat atcaccacca ctggtggtat gtccgctgaa
    24481 aataacctgt atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa
    24541 ccagctggtc atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg
    24601 attaaacggg ttttgcccca gaccgccata caactgttta gcgatgatca ccgcaaacac
    24661 cgtacccagt acgaccatcc accatggcgc gaggggggga atacttaccg ccagcaataa
    24721 gcctgtcagc aatgctgagt tatctttcaa cgttgcggct accgactgct tgcgtagttt
    24781 gagtacgaga gcttcggcta acagagcact gaccgacgcc aacaggatct gaacgagagt
    24841 accccaacca aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaacaccaa
    24901 cagcataatg cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa
    24961 taccatttag tcctcgttta caaccttttg ctgggcggct tttttggcct gaacgcgggc
    25021 aatagccgcg gcaactgccg ctttgcgcgg atcaatctgt tcttctggct ccgcattagc
    25081 ctgttgctgt tccagcttgc gtgctttggc acgggcaata gcagcttcga cggcggcttt
    25141 gcgcggat
//
LOCUS       DAYPHN010000063        23900 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000063 DAYPHN010000000
VERSION     DAYPHN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.063"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..254
                     /locus_tag="ACG0BW_002402"
     CDS             <1..254
                     /locus_tag="ACG0BW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFK7259353.1"
                     /translation="QFGAGTEFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIIL
                     SVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDGQIAQE"
     gene            complement(291..1139)
                     /gene="focB"
                     /locus_tag="ACG0BW_002403"
     CDS             complement(291..1139)
                     /gene="focB"
                     /locus_tag="ACG0BW_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFK7259354.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(1161..3173)
                     /gene="hyfR"
                     /locus_tag="ACG0BW_002404"
     CDS             complement(1161..3173)
                     /gene="hyfR"
                     /locus_tag="ACG0BW_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFK7259355.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATAEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLAHIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRCMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGTLLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMENLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(3203..3616)
                     /gene="hyfJ"
                     /locus_tag="ACG0BW_002405"
     CDS             complement(3203..3616)
                     /gene="hyfJ"
                     /locus_tag="ACG0BW_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFK7259356.1"
                     /translation="MTEECREIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTTLLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3609..4367)
                     /gene="hyfI"
                     /locus_tag="ACG0BW_002406"
     CDS             complement(3609..4367)
                     /gene="hyfI"
                     /locus_tag="ACG0BW_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFK7259357.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGETMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4364..4909)
                     /gene="hyfH"
                     /locus_tag="ACG0BW_002407"
     CDS             complement(4364..4909)
                     /gene="hyfH"
                     /locus_tag="ACG0BW_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFK7259358.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNTRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDEPLVAKEQL"
     gene            complement(4919..6634)
                     /gene="hyfG"
                     /locus_tag="ACG0BW_002408"
     CDS             complement(4919..6634)
                     /gene="hyfG"
                     /locus_tag="ACG0BW_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFK7259359.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6624..8204)
                     /gene="hyfF"
                     /locus_tag="ACG0BW_002409"
     CDS             complement(6624..8204)
                     /gene="hyfF"
                     /locus_tag="ACG0BW_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFK7259360.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHGLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(8209..8859)
                     /gene="hyfE"
                     /locus_tag="ACG0BW_002410"
     CDS             complement(8209..8859)
                     /gene="hyfE"
                     /locus_tag="ACG0BW_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFK7259361.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8871..10310)
                     /gene="hyfD"
                     /locus_tag="ACG0BW_002411"
     CDS             complement(8871..10310)
                     /gene="hyfD"
                     /locus_tag="ACG0BW_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFK7259362.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10327..11274)
                     /gene="hyfC"
                     /locus_tag="ACG0BW_002412"
     CDS             complement(10327..11274)
                     /gene="hyfC"
                     /locus_tag="ACG0BW_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFK7259363.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLVLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLPFGRAQELSLTCLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(11285..13303)
                     /gene="hyfB"
                     /locus_tag="ACG0BW_002413"
     CDS             complement(11285..13303)
                     /gene="hyfB"
                     /locus_tag="ACG0BW_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFK7259364.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVILVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVFIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEVQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(13303..13920)
                     /gene="hyfA"
                     /locus_tag="ACG0BW_002414"
     CDS             complement(13303..13920)
                     /gene="hyfA"
                     /locus_tag="ACG0BW_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFK7259365.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRIAASWFANGGEDPLSLTQEQR"
     gene            complement(14173..14643)
                     /gene="bcp"
                     /locus_tag="ACG0BW_002415"
     CDS             complement(14173..14643)
                     /gene="bcp"
                     /locus_tag="ACG0BW_002415"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFK7259366.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14643..15215)
                     /locus_tag="ACG0BW_002416"
     CDS             complement(14643..15215)
                     /locus_tag="ACG0BW_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFK7259367.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15361..16239
                     /gene="dapA"
                     /locus_tag="ACG0BW_002417"
     CDS             15361..16239
                     /gene="dapA"
                     /locus_tag="ACG0BW_002417"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFK7259368.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16256..17290
                     /gene="bamC"
                     /locus_tag="ACG0BW_002418"
     CDS             16256..17290
                     /gene="bamC"
                     /locus_tag="ACG0BW_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFK7259369.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17503..18216
                     /gene="purC"
                     /locus_tag="ACG0BW_002419"
     CDS             17503..18216
                     /gene="purC"
                     /locus_tag="ACG0BW_002419"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFK7259370.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18384..19247
                     /locus_tag="ACG0BW_002420"
     CDS             18384..19247
                     /locus_tag="ACG0BW_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFK7259371.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDEMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19262..21277
                     /gene="tmcA"
                     /locus_tag="ACG0BW_002421"
     CDS             19262..21277
                     /gene="tmcA"
                     /locus_tag="ACG0BW_002421"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFK7259372.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPVAENHCFPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     VHFAPRTDWHPATGTPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHEPQGDIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIVGALQYTHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21351..22049
                     /gene="ypfH"
                     /locus_tag="ACG0BW_002422"
     CDS             21351..22049
                     /gene="ypfH"
                     /locus_tag="ACG0BW_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFK7259373.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKVEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22130..22330)
                     /locus_tag="ACG0BW_002423"
     CDS             complement(22130..22330)
                     /locus_tag="ACG0BW_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFK7259374.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22358..23485)
                     /gene="dapE"
                     /locus_tag="ACG0BW_002424"
     CDS             complement(22358..23485)
                     /gene="dapE"
                     /locus_tag="ACG0BW_002424"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFK7259375.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVEWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23489..23845)
                     /locus_tag="ACG0BW_002425"
     CDS             complement(23489..23845)
                     /locus_tag="ACG0BW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFK7259376.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKIIDAASAAALMTEMPAIIKRPLLCTPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5959 a         6322 c         6416 g         5203 t
ORIGIN      
        1 tccagtttgg tgctggcacg gaattctgga gctgcttcgc ggtgtatctg attattcagg
       61 cgctggacgg caacctgtta gtacctgtgt tgttctccga agcggttaac ctgcatccgc
      121 tggtcattat tttatcagta gtgatcttcg gtggtttgtg gggattctgg ggcgtattct
      181 tcgccattcc attggcgacg ctgatcaaag ccgtgattca cgcctggccc gatgggcaaa
      241 tcgcgcaaga ataatcttca gcccgacgaa ctgtttcgcc gggctgattt ttagggttcc
      301 tgacgtaaat aaatagcccg ataacaaata ctcaccagca ctgcaccgcc gataatatta
      361 cccagcatca ccgggagcag attggcggta ataaaatggc tgaccgtcag tgccggaaaa
      421 ttgtctgcgc tactgtgcgc cagttgccag aaggggggag gggcgaaatg gcgaatggca
      481 atggcgaacg gaatcacaaa caaattggcg atacagtgct caaagccact ggcgacaaac
      541 agggtgatgg gcaaaatcat ggcgacgatt ttgtcgcata atgaacgccc gcaataactc
      601 atccacagcg ccaggcaaac catcagattg cacataatgc cgaggctgac agattcagta
      661 aatgtatgat gcattttgcc ctcggcgcag tgtaaaaccg cgactcccca catcgcgttt
      721 tcactcatca ccagcccgga aaaccagatt aacaaactga aacaggcaat acctgccaga
      781 ttaccgcagg ccaccagaag tgcattaatc agccatgttc gccagctaat aacgccccga
      841 ctttttgcca tcaccgtcat taccgacgag gtgaacaggc tggtgccgca aaccgccagc
      901 aaaataaagc cgagtgtaaa gcaaaggcca cccaccagat gggttaatgc ctgtgaagac
      961 ggggcatcgg cgataacgga aaggtaaaaa gtaaatccga tcgccataaa cacgccagcg
     1021 gacatcgcca ttaaaaagac cgacactttg ctgcgggcaa ttttatgggc ggcaatccgt
     1081 tcagcgattg ccgcttttct ggcgctcaac tgcaagtcga aagagagttt gtttctcatg
     1141 aatactcctg caaaagcaga ttacaacacc tcgcgaaccg agatccccag acgctgcatt
     1201 cgtgacagca gcgtggtgcg cttcatccct aatcgtgtcg ccgcgccacg gggcccggca
     1261 acaatgccat tggtttctcg caatacctga acaatgcgct gacgttcttc ttcgtcattc
     1321 tctggcgtcg tcgggtgcag caactgtgcc attgaactgc gaagcgctga atcttcattt
     1381 agcgtcggca gtaaacggct ttgtcggaca ttaagatgta aattcagact gttaccgcga
     1441 gtcagtagta ccgcccgctc aatcacgttt tccagctcgc gcacgttgcc cggccagtcc
     1501 cacgacatca actggcgtag cgcctcggtc gggatggcgt caattgcgcg attcatatgg
     1561 cgcgccattt tttgcgtgaa atgttttgct aaaagaggga tatcttccgg acggtcgcgt
     1621 agcgggggca attccagtgg gaagacattc aggcgataaa agagatcgct gcgaaactgg
     1681 cgatcttcaa ccatttgcca caaatcacgg ttggtggcgg caatgactct gacatttacc
     1741 gggatcgttc tactcccgcc gagacgctca atctcccgtt cctgcaatac gcgcagcagt
     1801 ttaggctgaa gttctaacgg cagatcgcca atttcatcga gaaacaacgt gccgccatcg
     1861 gcaatttcaa aacgaccacg atgggtatta atcgcaccag taaatgcccc tttgtcatga
     1921 ccgaataact cactttccag aagactggcg gggatggcag cgcagttgat tttgaccagt
     1981 ggcttgtcgc gtcgtgggct aagttgatgg atcgctctgg cgatcacctc tttgccggtt
     2041 ccggtttcac cacaaatcaa caccgtactg tcgctcttcg ccacaatatc tacctgctgg
     2101 agcaaatttt ccattgcctg gctttgatag ataatgtcac cgacgcccag attcgaaagg
     2161 agctgctcgc taagctggtg gttttcttgc tgcaagcttt cctgcaaatc ggtcatgctg
     2221 cgccaggcat cggcattgcc aacggcaata gcgatgcgat cggcaatatg ttgtagtagc
     2281 tggcagtttt cctcactaaa gagagtggaa gaggtatgcg ccagcaacaa tgtgcccggt
     2341 gtatggttgc caaaggtgag cggtataagg agcgcagatt cgcagccgtt agatgcaagt
     2401 aagtgtaata acgggtcgcg ttgccagaga aacagatcgt ctgcacggtg cgtcaaggtc
     2461 ggttgtcggg tttgtagcgt ttgtgtcagc aatacacttt cgccaggcat acagcgtggc
     2521 agacacgcac aatgtgaggc agaaagatcg ctgcaccaca ggctgaactt ctcgttcttt
     2581 cgatgatcgc ccagtaccat actgactgaa gccagaccga aaaaatgatg gatctcacga
     2641 gcgacgtcag cgatcagatc gtcgagatca agatgtgaca acactgcatt agtgatatcc
     2701 accagtaccc gatgatgatc gcgatctttc tccagcaaac gcagcgccac ctgctgttct
     2761 tcatcattga gccatgattg cagaaccgga gtgattatgt gtgccagcca gttaaacagt
     2821 gtgcaatcct ggggagtaaa acgactgccg tgggtgcgaa taaagcgtag ctccgccatt
     2881 ccactactgc cgagaaggac ggcgtgacgc gaataatcga ccatctcgcc ctgatactct
     2941 gctgttgccg tcacatcgct gccatcatca tggtagcggt aatagaagtt gcttttgaga
     3001 acgtgcagtt caataagttg taccgggctg aacggcggcg gcagcgggat gaaggcgcgt
     3061 aataaagacg ccttgccgtg tttctgcgct aaccgctccg cgagcattcc gtttaccgct
     3121 tcaattgtta ttccttgcgg cggggcaaac atcgcctcgt ctgacatagc catcaacagt
     3181 ccttattaat gagaaatcct tctcacgcaa tttttctggc catcagatag atggccggtt
     3241 cctgcacaat ttcatccaac agatgaatga attgaatgct acgcgcctgc cacacagacg
     3301 tcgcgtcatc tgccagtggc gcgaatgccc gggtcaacag ggtggtgtgg ctggcgtcaa
     3361 taacaatctc accaaaggtc atcaccccca gcatcttacg tcgggcttgc tcctcttcga
     3421 ccagtgcaag ccaatcttcg tattccgtca gtgggcagcg gaaggcgaca ttcagacaat
     3481 caatcacgcc aacgtgatgg ccgatagcta gcgagtaata cattacctgg gagctgtgtt
     3541 ccggcatctc gtcgctactg gcgacaaact tttttcgcag cgtccagaaa acaatttccc
     3601 tgcactcttc agtcatgtaa cccccggagt acgcgaaaga gttgctgaac gatactattg
     3661 agacgcggat cgtcggcatc gcgcaaccag gtgttaaccc gatttcctgt aggatcgtcg
     3721 cttaaatggc gcaggagccg atcgcaaatt tctcgcccct gacgatagcc cgccagccgc
     3781 ctcgcttctc gctcaatggc gatacgctgt gatggcggga tctgcggcca cagcggttgc
     3841 atggtctccc cggtgggatc gcgataatcc accgcgtgaa tcttctgttg cagcaaaccg
     3901 agcgccacgg cgaaaccgtg aatggtggcg gccggtgttg gcgggcagcc ggggatccaa
     3961 acatcaatgg ggacaatggt gtcgctgccg ccccagacgc tgtaaagatc gtggaaaata
     4021 ccgccgccga caccgcacgc gccgtaggaa acacaaattt tatgatcggg ggcagactca
     4081 tacgcccgaa gtgcaggcat acgcatcgcc cgggtgactg cgccagtaaa taacaaaata
     4141 tcggcgtgac gcggtgatga aacaacctta atgccaaaac gttctgcgtc gaatacgggt
     4201 gtgatggcag caaaaatttc gatttcacag gcgttgcagc cgccgcaatc gacgcgataa
     4261 acgtaagccg agcgacggat atcctgtagc aaatgctgct tcatcttttg cgtttgctcg
     4321 tccagcgtga tgggctggct gacatgttgc gtaagcactg gactcatagc tgctccttag
     4381 ccaccagcgg ttcatctgtg tcgtcgttga tcagcgtcgc gcgctgtttg cattccgggc
     4441 agacgctcgc ttgcgcccgt aacatttcgc ggttttgtgg tgcattttgt tgctgtgcca
     4501 acagttctgc agccagtgcg acggtttttt gcggggcaaa cgggcgttcg caacggctgc
     4561 aacgttgtag atggaacgtc gcgcgggtat agagatcggc tttattggtg acggtcagtt
     4621 caaagttatt ggtaagctga atggctctgg tcgggcacac ttcttcacaa cgtccgcagt
     4681 aaatacaacg ccccagatag agctgccagg tgcgcgtatt ttgctggtcg tcggtctgga
     4741 tagtcagcgc atttgccgga caagcacagg cgcaggcacc gcaggcaata cattggctgg
     4801 gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg aagggatatt
     4861 tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa cttcagcatc ttctgtcctt
     4921 acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta tacggcacgg
     4981 tttttgactg gcgcttgcgc acatcaacca gcgtcacacg gtcggtacag gagtagcagg
     5041 gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc agcatgtaac
     5101 gtagcactgg ccagttggcg taggtggcgg cacgacagcg ccagcggaac aatttttggt
     5161 tatcaccgag catgctccag tgcacgtctt caccacgtgg cgcttcaaca aagcccagcg
     5221 cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatct ggcatgttgt
     5281 cgagggcaaa ttccagcatt gccagcgaat caaacgtctc ttttacacgg accatcacgc
     5341 gggagaagac atcgccgccg gtaaaggtaa acagcgtttt gggaatattg ccgtagtcgg
     5401 cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gccgcggatc agcggcccta
     5461 cggggctata atcacgggcg atttgtcggt cgagaatgcc aatgccctga gtgcgttgtt
     5521 ccatattcgg cgtggcaagc agcatctcta ccagctccga cacgtcggct cgcatctcgc
     5581 gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga acaccaccaa
     5641 tcagattcag accgtaggtt ttacgcgacc cggtcagcaa ttccgccatt gtcatcgact
     5701 tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa accaacaaaa tggcaggaga
     5761 gaccaaggtt gagcaaatga ctgtgtagcc gttcgacttc cagcagaatc gagcgaatag
     5821 tatgcgctcg ttgcggcacc tcaatcccaa gtgcattttc aaccgagttg gtataggcca
     5881 cactgtgggc aaaaccgcaa atcccacaca cgcggtcgga taagaaggtc acttcgttgt
     5941 agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac aggcggtaat
     6001 cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacctggt tcatcggagg
     6061 tgatatgcag cgggccgaca gggatcaccc gcgcatcgct gttgccctcg ttgataaacg
     6121 gatacgtttc ggtatcagtc gtcggttctg ggcgcagtcg ataatccatc gcgtctttgc
     6181 gcagcggatg catatcttcc ggccagtcat cgggcaacac cagccgacgc tgatccggca
     6241 ggccaaccgg aatcagcccg tacatatcgc gaatttctcg ctcgccccag accgcggcag
     6301 ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagt gccttcacca
     6361 cgatccagca tttttcggcc ccttccatag aaagggcata ataaaccgcg taatgaccgt
     6421 taagtgtccg ctcgtcgttg ccaaacagga ctggaagcca gccatcatgt tgataataaa
     6481 gataatgcac aacgtcaggc agcagattga ttttcaccgt aatggtgacc tgttcaggcg
     6541 tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg gcgagaatcg
     6601 cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac agatgcggtg
     6661 cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg cgtccctgag
     6721 aggactatag tggaagcgcc tgccaggatc ctgatgacag gttgtggaat atgcgttccc
     6781 atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca gccaagttca
     6841 ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgcagccat ccgtaccagg
     6901 cccgccagca ccagcgttaa cagcaataac agcaggacga taatcagcag gtgattacgt
     6961 gccagtccgg cggtaacggt cataaattcg ctaagaaaaa tgttgaaggg cggcatcccg
     7021 gccagcgcca gcgcaccgcc gccaaacaac acggcggtaa atggcatgat tttgagcatc
     7081 ccacagacga cgttgagatc gcgcgtgccg tacttgagca gtacattgcc ggaaccgcag
     7141 aacaacagcg tttttgccag actgtggttt aaggtgtgca gcagcgcggc aaaaattccc
     7201 agcggcccgc caatgcctaa cgccaccgcg accagtccca tgttctccac gctggagtac
     7261 gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa atgccgccac ggcaaccgac
     7321 aacatgccga agatgagcaa caaccggttg gggaaatcgc tgccgatggc ctggcaaata
     7381 atgatgtaat agcgaataag caccaacagc gcgcagttca gcaatacggc ggagagcagg
     7441 gcgctgaccg gactcggcgc ttcactgtga gcatccggca gccaggcgtg catgggaaaa
     7501 agcccggttt tggtgccaaa gccaattagc acaaacacaa aggccaacag cattaatgtt
     7561 gggtcaagca aggacgattg cttaagaacc tcgctccaga atatcgccat ttctgcctgc
     7621 ggcataacgc tggcggcgtt ggcgtatacc agcacggtac cgaacagacc aaaagcgaca
     7681 ccaacagtac aaataatgat gtacttccat gcagcttcca gcgatgaacg ctgaccgtaa
     7741 atgcctacca gaaacgccga gcttaaggtg gtggcttcga ttgccgccca catcacaatc
     7801 aggttattgc tggtaacaac cagcagcatg gtgaacaaaa acagatggaa gaaaccgtag
     7861 taatcgcaca gcgtaacggg tgaaagctcg ccgtgtgcca cttcatgacg catgtagcca
     7921 atcgagtaaa caccggtgag aaagccaatc acaccaagaa tggcgaggaa caaaccgccc
     7981 agaccatcaa tgtgaagcca cagtcccgca gcgaatattt ctcccgcatc agcggcagtt
     8041 tggaccaccc acagtgccag aatcagcagc agtgtgatcc ctaagccatg tagtactgtc
     8101 accgtgcgag tcgcagagtg cccccgtttc cggcaggcaa aacagagcag cgaaaaaagc
     8161 agcggcgtga gcaggagtaa agcgaacatc acagaataac tcatctcatt atcccttcag
     8221 cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc catatttttc ttgccagtaa
     8281 caccatcaca atgacggcga agatggcgtc ggtagcgata ccaatttcca ccagttccgg
     8341 tgcacgccag gcaagaagcg ccagtaccag atgggagccg ttttccatca ggcagtaacc
     8401 aaaaatttgc cgcaggatat tgcgctggct gacaatgcac agcaggccaa gcagaaaatg
     8461 acctaacgcc accgccagcg ccggtttcag cccggtagcc atcggtagct tcacgggctg
     8521 aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc agtgtcatca ttgccggacc
     8581 gaataacgct ttttccggga tgttctgggg aatatttcgt gcagcgtaag tcatgattaa
     8641 cggtaccagc agcactttgg tgataaaggc gctggcggac cagatcagca gttgctctgc
     8701 ggcgaacagg cacgagagag tggcgaaaat agacaccagc accagcgact ggcaggcgta
     8761 aaatccgcat gacaggcgat agcttttgac gctaatcaca aacagcgatg tcagcatcat
     8821 cagtcccgcc agattattta cgatcataga accggtcatt catcatctcc ttactgcaac
     8881 caggtcgcgg cgattacgct ggaaatcagc gacatcacaa tcagtacaat caacaccagg
     8941 cgcattgatc ctggcagcgg tgcggcatcg gcgacggcct cgctcggttt gccaggcacg
     9001 acgcgaccaa accagcgaat aaaccaggcg aaactggcga ccgattcaat catcagcaga
     9061 atcatggcgg gcagcaggat ccagtactcc actgacaacg caaaaccggc ggcaaacagc
     9121 gggaatttac tgaagaagcc gttgaacggc ggcacgccgg taattgccag cgctgccacg
     9181 cagaaaccca cgccaggcaa cggcagggtg tgcaatacgc cacgcagacg cggcaacaag
     9241 cgcgtgccgc agctgtaact cagcgcacct gctacaagga aaaacaggct tttagcgaac
     9301 gcgtggttga cgatgtaggc gatgctgccc tccagcgcca gccgcgaacc gaagatggag
     9361 agcgacaagc cgaaaaacat ccagccaagt tgagtgatgg tcgaccaggc cagcaaccgc
     9421 ttcatatcct gctgtggcaa atacatcagg aagccataaa gaatggtgac cagtgccata
     9481 accatgccaa cgccgccaat cacatgcggg atattgccgc cgtcgataat ggcacgggca
     9541 aaaatgtaaa cgcccacttt caccatcgat gcggcgtgga gataggcgct gatcggtgtt
     9601 ggcgcttcca ttgcatccgg tagccacgct tgcatcggta gctgggccga tttcccccac
     9661 gcggcaaaca ggatgccacc ataaaccaga tagcgtgcgt cgccgtgtaa ctcgctcatc
     9721 gcgctgagcg caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag atacaacccc
     9781 agcgaaccga tatgagtgat aagcaacgct tttagtgctg aacgctgcgc tttatcgctc
     9841 tggtaataac tgatcaacgc ccaggagcag ccgcccgtaa tttcaaaaaa caacaactga
     9901 ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacac cagcaaaaat
     9961 gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt cagataaccc
    10021 gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat cagcgtactc
    10081 acgcgatcaa tgaccaggcc aaacaacgcc acatccccca cgttgaccaa cgtcaacgtg
    10141 acggcaactt tatcggcctg ataaaacgcg gagatcagtg acaacatgca cagcgtggtc
    10201 agcgcggcga acaaaactcc ccattcggcg gcccgacgtt gtggcgaaaa cgaaacgacc
    10261 agtgcgccga taaaaggcag caataacgtc gtcagagcaa gattttccat attccgtcag
    10321 tgctccttac agaccggtta accagaagac ccaggcaagc gcagcaaggc tgaagccaag
    10381 ccaggtcaca tggtgaatga gtaaaaaacg cccgcgtgcc agcgtgtttt ctgcgattga
    10441 ggccagtaca aaaatcagca aaaccttgag cagcgtaacg acaagtgaag tcagcaggca
    10501 ggtgagagaa agctcttgcg cgcgcccaaa gggcagaaac agggccacaa acagtgatgc
    10561 catcacgacc tgtttcagcc ccagccccca tttcgccagc gccagcccgg caccggaata
    10621 ttcggtcagc gggccttcct gtaattcctg ttctgcttca gcaacatcaa agggaatttt
    10681 tcccatctca atgaagcagg caaaaccaca ggccagtaac gccagtacgg tggttagcgg
    10741 cgagttccag cccgtcgcca gcgtattgct gatcatctcg atatgcgtgg aatccgctat
    10801 cagcgccaat accagcagtg agagaatgag catgggttcg accagaatgc cgagcgtcaa
    10861 ctcgcgactg gcaccgactc ccgcaaaggg gcttccggta tccagcccgg aaagagcaaa
    10921 gaaaaaacga aacagggcaa gaagatagat aagggtgatc agatcgccgc cgcccgcaaa
    10981 aggggaaaca gtaataaata gtggtagggc catcgccagc accagcatgc tgctgattaa
    11041 tacccacggc atcaggcgga acatcagacc tgaagatgtc ggcgcaactt cctggcgttt
    11101 aaacagtttg tggatatcgc gataatcctg ccagatcccc ggtccgcgac gggagtgcat
    11161 acgcgcgcgt atctgccggg aaatccccgt aaaaagcggg gttagcacca gcagaatcac
    11221 ggcctgtgct aacgcaaaaa tgaccagata tccgtcgcaa agagtttgtc tcatggtgat
    11281 ttccttagac ggcaatagcg attagcagca caaccagtgc ggcgaccaca tacaggcaat
    11341 agagacgaaa gtcgccgctt tgcagatgct gtacttcttt ggccagccgt tgggtggcgc
    11401 tcacgatggg gcggatcacc cgctcatccc agaagggttc tgtgctctga gccctggcgg
    11461 tgacgtgcgc aagaccttta tttagcctca gcgtagggtc gagctgttgt cgtagacgaa
    11521 atagcgcaca aaagaccaca cgcagcggct gcatcacgcc attgcctgac ggggccatcg
    11581 cattttccca gccgtagccg catgcccagg catctcctgt gcgacgaaac gctgcgcggc
    11641 gcgaacgaca ccacagccag taaaggccag gcagcaaagg catcgccagt agtaacagca
    11701 acagtaatga gggagtgacc cgtgtatgaa acgtgcctgg tacaagtgct attccgctgg
    11761 cgacagtgac gggaggggta ttggtaaacg catgggcaat gtgcattatc ttcggtgcca
    11821 gccaactggc actaagcgca atgaatacgc agagtgccgc gagcagtagc atggcgacga
    11881 tcatcgtatt cggcacttcc tgtgcctctt cagcgtgtgt actgcgcggc gctccacaga
    11941 aagtaatacc gtacattttg acgaagcaca ttactgccag cccaccagtg actgccagca
    12001 tcaccatagc aataggaccc gcaagttgta gcgctacggc ttccacacga cttagtgaga
    12061 acagcgactg ccaggtgtac cattcgctga taaaaccatt cagcggagga atagcagata
    12121 tcgcgaggca accaatcagg catgctgcgg ctgtccacgg catacgtttc gccagtgccc
    12181 ccattttttc catgtcgtgg gtatgcaaac gcgaaataat cgcacccgcg ccgagaaaca
    12241 gcagcccttt gaacagcgca tggttgagca gatgaaacag cgcgccgagc agtccaacaa
    12301 cggtgagcag cgggtcgtgc agtgacagac cgaccatcgc cacaccgacc gccagcaaaa
    12361 taatgccgac gttttcgacg gtactccagg ccagcagccg tttgatatcc tgttccgcca
    12421 gcgcatacag cacgcccagg agcgcggaga ttgcgccgat cgccatcacc agaatgcccc
    12481 accacagcgg caaacccgtt tgcgccagca ggtccatcgc tactttcagg atgccgaaaa
    12541 taccgatttt gaccattacg ccagacatca acgccgaagc gtgcgatggt gcggcagggt
    12601 gagcgcgtgg caaccagctg tgcaacggca tcatcccggc tttcgcgcca aaaccaaaga
    12661 aggccagcag gaacaccgcc gacgccagcc ccggagaaag tgaaagcgtg cggaaactgg
    12721 caaaatcgag gctgccgctt tcgcgcccca tcagcaagaa ggcgatcata atcagtaccg
    12781 atccggcgtg ggcgataaaa aagtagagca tgccagcgtt gattgacgtt ttatcctgcc
    12841 tggcaatgac cagaaaccag gaagacagcg acatcatttc aaacagcacg atgaaccaaa
    12901 aagcgttgtc catcaccagc agggcaacca tcgatgcgat gaaaagattc ataaagaagc
    12961 ccatcgccgc cgcgcctttg ccctcgtatt cgcgcatata agtcaatgaa tagagcgaac
    13021 aaaccaccac cagcaaggag ataaccagca ccataaatgc cgagaggcta tccattcgca
    13081 gggttaaatc ggcaaagtca aaaggcgtgg caaaacgggc gactaaaatt acgcctgcat
    13141 gtaattgcgt aattccgctg atgatgccaa tcacgccacc caccagcgat gtgatgccgg
    13201 aaagcttaat agccagtctg tccagaccga gtaaaaacag cgaagccaga ctggcaaaca
    13261 gatagaggat cagcgaccag gttaataatt gcagggcatc cattagcgtt gctcctgagt
    13321 gagggaaagg ggatcctccc cgccattggc aaaccagctt gcggcaatgc gctgtttttc
    13381 tttcatctga cgttgcaggc tatcgtcggt gatcagccgt aacgcctgat tcgggcaagc
    13441 gcgaacgcag gccggacctt ctggcaagaa atcacacagg tcgcatttca ccgcgacggt
    13501 ctggacaccc ggttcccagc gcagcaaagc atgttgggtc ggcgcgtttt cttcgccgtc
    13561 tttgagtgag ccctcagcct gaaaaacata ttgcgcaggg gcattcaccg gacggcttcc
    13621 tgaagcgctg attgcgccaa atgggcagac cacggcacaa agcttgcagc caatacagag
    13681 gctttcgttg agttggatcg catcatccct ctgggagatg gcattgaccg ggcagacctg
    13741 caggcaaggg gcttcctcac agtgatgaca cacgacaggg gcagtgagtg ttgacgtctt
    13801 cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt ttatgcacgt ccgaacaggc
    13861 agcgagacag gtattacatc ctgtacacca cagtggttcg gccaccacaa agcggttcat
    13921 gtcggctcct tgaaaatgat atgaaacatt gcatttcata ttgcataaag gatgccagtc
    13981 ttattaattt aatatattca ataggttatg aattaactgt attctgaacg gtacaaaaat
    14041 gatggtcatt aacgttcagc aaaaaatgaa ttcgacaccc gtgtcgcagg ggaaagcgag
    14101 aatgtgatct tgatcacgag aagtgaggtg tgctgaccgc aagcgcagcc agcacggaat
    14161 ggagcaaagt aatcaggcgt gttctttcag ccagttcagc acaacgtcgt ggtgattgct
    14221 ggttttgaaa tcgtcaaaga catgttcgat tttgccatca gcgtcaatca ggaagctgat
    14281 gcgatgaata ccatcgtagg ttttgcccat gaaggacttt tcaccccaga cgccgaattg
    14341 ttcgcacacc tggtggtcct catcagacag gagcgtaaag ttaagcagct ctttttccgc
    14401 aaaacgggag agtttttcgg gtttatcggt gctgataccc agcacatcaa cgcccgcttt
    14461 tttcaactca tccatgttat cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat
    14521 ggctttcggg tagaaataaa ccagaacacg ctgtccctgg aagtcggtca aattaacttg
    14581 ttctccgtct tgatccggca agctaaattt cggtgcgata tcaccggctt tcagtggatt
    14641 cattacttaa ctccatcctg ttcatcatgt tgggaataat tgacgacgtt aatactgcct
    14701 tgtgcattga gttctgtaca tagggctttg aacgcttgct caatatttgc tgcgtccgca
    14761 gatgcggggc tgtgggcggt tatctgaata tgcaactgcg cagcccgttc attttcagca
    14821 ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg
    14881 aagcgttcaa ttaaatgcgg ggagtctgcc acatcgacct gaacccagac agatgctggc
    14941 attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga ttaaaagatc cagctcggca
    15001 cctttcaacg gtaacgttga ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc
    15061 atgataaacg tgaactcttc tcccagcatc gccaggcgac tgtcttcaat attacagccg
    15121 caactactga catgacgggt gatggtgttc acaattccag ggcgatcggc acccaacgca
    15181 gtgatcacca gataatgttg cgatgacagt gtcaaactgg ttattccttt aaggggtgag
    15241 ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc
    15301 ttgcttttaa tgccatacca aacgtaccat tgagacactt gtttgcacag aggatggccc
    15361 atgttcacgg gaagtattgt cgcgattgtt actccgatgg atgaaaaagg taatgtctgt
    15421 cgggctagct tgaaaaaact gattgattat catgtcgcca gcggtacttc ggcgatcgtt
    15481 tctgttggca ccactggcga gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg
    15541 atgatgacgc tggagctggc tgacgggcgc attccggtga ttgccgggac cggcgctaac
    15601 gctactgcgg aagccattag cctgacgcag cgcttcaatg acagtggtat cgtcggctgc
    15661 ctgacggtaa ccccttacta caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa
    15721 gccatcgctg agcatactga cctgccgcaa attctgtata atgtgccgtc ccgtactggc
    15781 tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc
    15841 aaagaggcaa cagggaactt aacgcgtgta aaccagatca aagagctggt ttcagatgat
    15901 tttgttctgc tgagcggcga tgatgcgagc gcgctggact tcatgcaatt aggcggtcat
    15961 ggggttattt ccgttacggc taacgtcgca gcgcgtgata tggcccagat gtgcaaactg
    16021 gcagcagaag ggcattttgc cgaggcacgc gttattaatc agcgtctgat gccattacac
    16081 aacaaactat ttgtcgaacc caatccaatc ccggtgaaat gggcatgtaa ggaactgggt
    16141 cttgtggcga ccgatacgct gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag
    16201 acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa gtttagggag atttgatggc
    16261 ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt gtttcgcttg ttttattact
    16321 cgctgcctgt agttctgact cacgctataa gcgtcaggtc agtggtgatg aagcctacct
    16381 ggaagcggca ccgcttgcgg agcttcatgc cccggctgga atgattttgc cggtgacctc
    16441 cggtgattat gcaatcccgg tgaccaacgg tagtggtgct gtcggtaagg cgctggacat
    16501 tcgtccacca gcccagccgc tggcactggt ttctggcgcg cgtacccagt tcacgggcga
    16561 taccgcttca ttgctggtgg aaaatggtcg tggcaatact ctgtggccgc aggtggttag
    16621 cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat gatgctggtc agacactgac
    16681 caccgattgg gtacaatgga accgtctgga cgaagacgag cagtatcgtg gtcgttatca
    16741 aatctctgtt aagccgcagg gttatcagca ggcggttacg gttaaactgc tgaacctgga
    16801 acaggcgggc aaaccggttg cagacgcggc ttccatgcag cgttacagca cggagatgat
    16861 gaacgttatt tccgccggtc tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa
    16921 tcgtgcctcc accactatgg acgtacaaag tgcagctgat gacaccggtt taccaatgct
    16981 ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg ccagcggcgc tggaaaaagt
    17041 gggcatgaaa gtgaccgaca gcacccgttc gcagggcaac atggccgtaa cttacaagcc
    17101 gctgtctgac agcgactggc aggaactggg cgcgagcgat ccaggcctgg catccggtga
    17161 ctataaactg caggttggcg atttagataa ccgcagcagc ctacagttta tcgatccgaa
    17221 aggtcatact ctgactcaga gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt
    17281 tagcaagtaa aaatacaggg ctggagtcat ccggcccttt tttctgatat gatacgcaaa
    17341 cgtgtgcgtc ggcaggaaaa cgcgatttta gcggtaattt gcactaaatt tgtttgtcgg
    17401 acgtaggtcg gataaggcgt tcttgccgca tctgacaaaa catccggcac accagacagc
    17461 aaaagatttt aaaacgttaa ttcacaccca ggagtgataa agatgcaaaa gcaagctgag
    17521 ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa acccggacct gttggtgctc
    17581 gaattccgca atgatacgtc agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa
    17641 ggtatggtga acaacaagtt caactacttc attatgagca aactggctga agcgggtatc
    17701 ccgactcaaa tggagcgtct gctctccgat accgaatgtc tggtgaaaaa gctggatatg
    17761 gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct ctctggtgaa acgacttgga
    17821 atcgaagaag gtattgagct gaacccgccg ctgttcgatc tgttcctgaa aaacgacgcc
    17881 atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct ttggctgggt aagtaaagag
    17941 aacctggcgc gtatgaaaga gctgacctac aaagcgaacg acgtgctgaa aaaactgttc
    18001 gatgatgctg gtctgatttt ggtcgacttt aaactggagt ttggcctgta caaaggtgaa
    18061 gttgtactgg gcgatgagtt ctccccggac ggcagccgcc tgtgggacaa agaaacgctg
    18121 gagaaaatgg acaaagaccg tttccgccag agcctcggtg gcctgatcga agcctatgaa
    18181 gccgtcgccc gccgcctggg tgtacagctg gactgatttt tctgttcatc atcttgccgt
    18241 gcagctggca cggcaagaca accgctctcg gaagatgtgc attgaacgct cattcctccg
    18301 ccatttcatc ccgctttcct gtggtaatct tcctctgaac cacctacgat catttctata
    18361 atgaatatat tgttgaggta tctatgcgtt ggcaagggcg acgtgaaagt gacaatgttg
    18421 aagacaggcg caacagctct ggtggtccgt caatgggcgg tccaggtttt cgcctgccaa
    18481 gcggtaaagg cgggctgatt ttactgatag tcgtgctggt tgcaggctac tatggcgttg
    18541 atttaaccgg gttgatgacc ggacagccgg tttcccaaca gcaatcaaca cggtcaatta
    18601 gtcctaatga agacgaagcc gcaaaattca cttcggtgat tctggcaacc acggaagaca
    18661 cctggggaca gcaattcgag aagatgggta aaacctatca gcaaccgaag ctggtcatgt
    18721 accgtggaat gacgcgcacc ggctgcgggg cgggccagtc cattatgggg ccgttctatt
    18781 gcccggcgga tggcacggtt tatatcgatc tctccttcta tgatgaaatg aaagacaaac
    18841 ttggcgcgga tggcgatttt gcccaggggt acgttatcgc ccatgaagtc ggtcatcatg
    18901 tgcagaaact gttaggcatc gagccgaaag ttcgtcaact gcaacaaaac gcgacgcagg
    18961 cggaagtgaa ccgcttatct gtgcgtatgg aactccaggc cgactgtttt gctggtgtct
    19021 gggggcatag tatgcagcag caaggcgttc tggaaaccgg cgatctggaa gaggcgctga
    19081 acgcggcgca ggccatcggc gatgaccgtt tacaacagca aagtcagggg cgagtagtac
    19141 cagacagttt cactcatggc acttctcagc aacgctacag ctggtttaaa cgtggtttcg
    19201 acagcggtga tccggcacaa tgcaatactt ttggtaaaag catttaactt ccggggcagg
    19261 gatggctgaa ctgactgcgc ttcacacatt aacagcgcaa atgaaacgtg aagggatccg
    19321 ccgcttgctg gtgttgagcg gggaagaggg ttggtgtttt gatcatgcgc ttaagttgcg
    19381 tgatgcctta cctggcgact ggctgtggat ttcgccgcag ccagtcgctg aaaaccactg
    19441 ttttccctcg gcgctacaaa ctttacttgg gcgcgaattc cggcatgcgg tattcgacgc
    19501 ccgccacggc tttgatgccg ctgcctttgc agcacttagc ggaacgttga aagcgggaag
    19561 ctggctggtg ttgttactcc ctgtatggga agagtgggaa aaccaacctg atgccgactc
    19621 gctgcgctgg agtgattgcc ctgaccctat tgcgacgccg cattttgtcc agcatctcaa
    19681 acgtgtactt acggcgaata acgacgccat cctctggcgg caaaaccagc cgttcacgtt
    19741 ggtgcatttt gctccccgta ctgactggca ccccgcgacc ggcacaccac aaccagaaca
    19801 acagcaactc ttacagcagt tactgaccat gccgccgggc gtggcagcgg taacggctgc
    19861 gcgtgggcgc ggtaaatcgg cgttggcagg gcaactcatt tctcgtattg cgggcagtgc
    19921 gattgtcacc gcgcccgcaa aagcggcaac ggatgtactg gcacaatttg cgggcgagaa
    19981 gtttcgcttt attgcgccgg atgccttgtt agccagcgat gagcaagccg actggctggt
    20041 ggtcgatgaa gccgcagcca tacctgcgcc gttgttgcat caactggtat cgcgttttcc
    20101 tcgaacgttg ttaaccacta cggtgcaggg ctacgaaggt accggacgtg gttttttgct
    20161 gaaattttgc gctcgctttc cacatttaca ccgttttgaa ctgcaacaac cgatccgctg
    20221 ggcgcaggga tgcccgctgg aaaaaatggt cagcgaggca ctggtttttg acgacgaaaa
    20281 cttcactcac gaaccacaag gtgacatcgt catttctgct tttgaacaga cgttatggcg
    20341 aagcgaacca gaaacgccgt taaaggtata tcagctattg tctggcgcgc actaccggac
    20401 ttcgccgctg gatttacgcc gcatgatgga tgcaccaggt caacattttt tacaggcggc
    20461 tggcgaaaac gagattgccg gagcgctgtg gctggtggat gagggcggat tatctcaaga
    20521 actcagtcag gcggtatggg cgggttttcg tcgcccgcgg ggtaatctgg tggcccagtc
    20581 gctggcggcg cacggcagca atccactggc ggcgacattg cgcggacggc gggttagccg
    20641 gatagcagtt catcctgctc gtcagcggga aggcacaggg cgacagctta ttgttggtgc
    20701 tttgcaatat acgcatgacc tcgactatct ttcggtgagt tttggttaca ccgaggagtt
    20761 atggcgtttc tggcaacgct gcggttttgt gctggtgcga atgggtaatc atcgtgaagc
    20821 cagcagcggt tgctatacgg cgatggcgct gttaccgatg agtgatgcgg gtaaacagct
    20881 ggctgaacgt gagcattacc gtttacgtcg cgatgcgcaa gctctcgcgc agtggaatgg
    20941 cgaaatgctt cctgttgatc cactaaacga tgccgtcctt tctgacgacg actggcttga
    21001 actggccggt tttgctttca ctcatcgtcc gctattaacg tcgttaggtt gcttattgcg
    21061 tctgctacaa accagcgaac tggcattacc ggcgctgcgt gggcgtttac agaaaaacgc
    21121 cagtgacgcg cagttatgta ccacacttaa actttcaggc cgtaagctgt tactggttcg
    21181 ccagcgggaa gaggccgcgc aggcgctatt cgcacttgat gatgttcgca ctgagcgtct
    21241 gcgcgatcgc ataacgcaat ggcaattttt tcactgactc cttcaatttt ctcccatggt
    21301 cattgtgagt cagcggcgta aaattaatga catcagttaa ggagttcgcc atgaaacatg
    21361 accattttgt tgttcaaagc ccggataaac ctgcacaaca gttgttactg cttttccacg
    21421 gtgtcgggga taacccggtg gcaatggggg aaataggtag ctggtttgcg ccgctgtttc
    21481 ctgatgcgct ggtggtgagt gtcggtggcg cggagccgag cggtaatccg gcggggcgtc
    21541 agtggttttc ggtgcagggt attacggaag ataatcgcca ggcacgtgtg gatgccatca
    21601 tgccgacgtt tattgaaacg gtgcgctact ggcagaaaca gagcggagta ggggctaacg
    21661 ccacggcact catcggtttc tctcaaggcg cgattatggc gctggagagc attaaagtcg
    21721 aaccgggcct tgcttcgcgc gtcattgcct ttaacggacg ctatgccagc ctgccggaaa
    21781 ccgcgtctac agccacgaca attcacttga ttcatggtgg tgaagatccg gttatcgatc
    21841 tggcgcatgc ggttgcggcg caggaagcgt taatcagtgc cggtggtgac gtgacgctgg
    21901 atatcgtgga ggatttaggc catgcgatcg acaatcgcag tatgcaattt gccctggatc
    21961 acttgcgtta taccattccg aaacattatt tcgacgaagc gctaagtggc ggtaagcctg
    22021 gcgatgatga tgtgattgaa atgatgtaat aatagccggg taggcctgat aagactgttt
    22081 caggcatcat ggccggatgt ggcgtgaacg ccttatccgg ccactcagct cacttcttcg
    22141 gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg
    22201 ggggaagctc cggcttgttg gcgagaaatt tcttatggtc gacgcgcttg agatctttaa
    22261 tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa tatttagcca
    22321 gccagtccat gcttatttcc tcttgcagaa ccactcatca ggcgacgagc tgttccatga
    22381 tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat tcattaattt
    22441 tatgaatagt tgcattgacc ggcccgagtt ccaccacctg cgcccccatg cgagcaataa
    22501 agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca ttatagtgct
    22561 caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg
    22621 aaagccacca ttccaccgta taacgcagtt gatgtttttc aagcagggca agcacctgcg
    22681 ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc acaaacagtt
    22741 cacccggaat aacgttgtta ctgcccgttc ccgcctgaat attggcaatc tgcatactgg
    22801 tcgccgggaa gaattcattg ccctgatccc actcaatagc cactaattca ttaaggaaag
    22861 gtgctgcgcg atgtaccgga ttgtcagcca gatgtgggta ggcaacatgc ccctgcacgc
    22921 catgaatggt gaggttgcag gttaacgatc cgcgacgacc atttttcacc acatcaccta
    22981 ccacttcgat actcgacggt tcgccaacca ggcagtaatc gagacgctca ttacgtgcca
    23041 tcaacgcttc gacgactttt accgtaccgt tatgggcact ggcttcctca tcagaagtga
    23101 tcagaaatgc cagtcgacct gcatggttgg gatgttgtgc gacaaagcgt tctgccgcta
    23161 ccaccatcgc tgccagcgag cctttcatat ctgccgcacc gcgcccgaat aacatgccat
    23221 cacgaatggt gggttcaaac ggcggattga tccaacgatc ggcgtcgcca ggcggcacca
    23281 cgtcggtatg cccggcaaag gccaacgttt caccttgccc acgccatgcc caaaaattct
    23341 gcgtatcggc aaaatccatg cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca
    23401 acaaagcctg acatcctgcg tcatcaggac tcagggaagg gcggcgaata agctgttgtg
    23461 tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaattgc tgataactgg
    23521 aatcactgaa acccagcagc ataggcttac cgggcgtgca gagcaatgga cgtttgataa
    23581 ttgcaggcat ttcagtcatt aatgccgccg cagaggccgc atcgatgatt ttattgcggg
    23641 tggtttcgtc cagtttacgc caggttgtac cacgggtgtt gagtaacgct tcccagccta
    23701 attcgttaat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg taatcatgaa
    23761 aacgataatc gatgttattg gcttccaacc aacggcgagc ctttttaatg gtgtcacaat
    23821 ttttgatgcc gtaaagtgta accataatga atcctttttc gatttatgag aataaatact
    23881 catttaagag tgaaaacatt
//
LOCUS       DAYPHN010000064        23595 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000064 DAYPHN010000000
VERSION     DAYPHN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.064"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(160..1809)
                     /gene="actP"
                     /locus_tag="ACG0BW_002426"
     CDS             complement(160..1809)
                     /gene="actP"
                     /locus_tag="ACG0BW_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFK7259377.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1806..2120)
                     /gene="yjcH"
                     /locus_tag="ACG0BW_002427"
     CDS             complement(1806..2120)
                     /gene="yjcH"
                     /locus_tag="ACG0BW_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFK7259378.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2593..4551)
                     /gene="acs"
                     /locus_tag="ACG0BW_002428"
     CDS             complement(2593..4551)
                     /gene="acs"
                     /locus_tag="ACG0BW_002428"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFK7259379.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVPSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLIEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQDIAMPS"
     gene            4943..6379
                     /gene="nrfA"
                     /locus_tag="ACG0BW_002429"
     CDS             4943..6379
                     /gene="nrfA"
                     /locus_tag="ACG0BW_002429"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFK7259380.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTATPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQERKNGLLSQ"
     gene            6424..6990
                     /gene="nrfB"
                     /locus_tag="ACG0BW_002430"
     CDS             6424..6990
                     /gene="nrfB"
                     /locus_tag="ACG0BW_002430"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFK7259381.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTLQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6987..7658
                     /gene="nrfC"
                     /locus_tag="ACG0BW_002431"
     CDS             6987..7658
                     /gene="nrfC"
                     /locus_tag="ACG0BW_002431"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFK7259382.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7655..8611
                     /gene="nrfD"
                     /locus_tag="ACG0BW_002432"
     CDS             7655..8611
                     /gene="nrfD"
                     /locus_tag="ACG0BW_002432"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFK7259383.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVMLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSCTSVMSMGVMLFQ
                     FYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAICQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8631..10349
                     /gene="nrfE"
                     /locus_tag="ACG0BW_002433"
     CDS             8631..10349
                     /gene="nrfE"
                     /locus_tag="ACG0BW_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFK7259384.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGACARICWRWALPGW
                     GALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10342..10725
                     /gene="nrfF"
                     /locus_tag="ACG0BW_002434"
     CDS             10342..10725
                     /gene="nrfF"
                     /locus_tag="ACG0BW_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFK7259385.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMILILWRVRAKR"
     gene            10722..11318
                     /gene="nrfG"
                     /locus_tag="ACG0BW_002435"
     CDS             10722..11318
                     /gene="nrfG"
                     /locus_tag="ACG0BW_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFK7259386.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPKAQLQALQDKIRTNPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11660..12973
                     /gene="gltP"
                     /locus_tag="ACG0BW_002436"
     CDS             11660..12973
                     /gene="gltP"
                     /locus_tag="ACG0BW_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFK7259387.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13278..13967)
                     /gene="yjcO"
                     /locus_tag="ACG0BW_002437"
     CDS             complement(13278..13967)
                     /gene="yjcO"
                     /locus_tag="ACG0BW_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFK7259388.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(14061..16208)
                     /gene="fdhF"
                     /locus_tag="ACG0BW_002438"
     CDS             complement(14061..16208)
                     /gene="fdhF"
                     /locus_tag="ACG0BW_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15789..15791),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFK7259389.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVESIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16406..17872)
                     /gene="mdtP"
                     /locus_tag="ACG0BW_002439"
     CDS             complement(16406..17872)
                     /gene="mdtP"
                     /locus_tag="ACG0BW_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFK7259390.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDT
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            complement(17869..19920)
                     /gene="mdtO"
                     /locus_tag="ACG0BW_002440"
     CDS             complement(17869..19920)
                     /gene="mdtO"
                     /locus_tag="ACG0BW_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFK7259391.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIASLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRTITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ATEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGADWEGIHTCMLTCVIVANPNVG
                     SSYQKMALRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19920..20951)
                     /gene="mdtN"
                     /locus_tag="ACG0BW_002441"
     CDS             complement(19920..20951)
                     /gene="mdtN"
                     /locus_tag="ACG0BW_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFK7259392.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20970..21245)
                     /gene="ytcA"
                     /locus_tag="ACG0BW_002442"
     CDS             complement(20970..21245)
                     /gene="ytcA"
                     /locus_tag="ACG0BW_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFK7259393.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLLWLAFF"
     gene            complement(21454..23439)
                     /gene="yjcS"
                     /locus_tag="ACG0BW_002443"
     CDS             complement(21454..23439)
                     /gene="yjcS"
                     /locus_tag="ACG0BW_002443"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFK7259394.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQIN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5314 a         6204 c         6416 g         5661 t
ORIGIN      
        1 acaagaaaga aaaggcatat tctctgcatt atcattttct acgactgtca aaaatcgctc
       61 attttttaat gcgtttattt gtttaatatt atgggaaaag gtgatgcatt ttgcagagga
      121 agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgcgcgc ggccttgctc
      181 aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac gctcacgcgc
      241 gccttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg ccacgctgat
      301 agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga ggatctgtac
      361 ccaaatcgtc gggccgagga tcatcagcac tacagcggta atcagcccca gccagccgcc
      421 catcattgcg ccacgcgtgg tcagttttga ccagtacatg gaaagcagaa tgattgggaa
      481 gttacagctc gccgcgatgg caaacgccag gcctaccatg aaggcgatgt tctgattctc
      541 aaacagcacg ccgaggatta ttgcaatcac gcccaaaatc aggacggtga ttttcgatac
      601 ccgcagctct tcacgttcgg tcgccccttt tttgaagacg ttagcgtaca ggtcgtgaga
      661 caccgccgat gcgcccgcca gcgtcagacc cgcaaccacc gcaagaatgg tggcgaaagc
      721 aaccgctgaa ataaagccga ggaacaggtt gccacccact gcattcgcca ggtgaactgc
      781 cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg gattcgcacc
      841 aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt agcccataaa
      901 tccggtagcg tagaacacgc tcttacgcgc ttcgcgggca tcgctgaccg taaagaagcg
      961 catcagaatg tgcggcaagc cagccgtacc aaacatcagc cctagcccca gagagagcgc
     1021 ggagatggga tctttcacca gcccgcccgg cttcatgatg tcgacacctt tcgggtgtac
     1081 cgccatcgct tcgctgaaca gattgttgaa gctaaagccg acgtgtttca tcaccataaa
     1141 ggccataaag ctggcaccga acagcaacag cacggctttg ataatctgta cccaggtggt
     1201 cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca gtaccaccgc
     1261 aatatgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta ccatctgagc
     1321 gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac gaatcggccc
     1381 ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt tacgcagacg
     1441 ttcggcaatc aggaacagaa tgattggcca gcccaccagg aagcccaacg agtagatcag
     1501 cccgtcatag ccggaggtaa acaccagcgc ggagatcccc aagaatgagg cggcggacat
     1561 atagtccccg gcaatcgcca gcccgttctg aaaaccggtg atattgccgc ctgccgtgta
     1621 gtagtcatta cgcgaacgta cgcgttttga cgcccagtag gtaatgccga gcgtaaacac
     1681 gacgaaaatc aggaacataa taatcgcctg ccagtttgtt ggctggcgct ctacggcccc
     1741 gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg ccgtcagaac
     1801 tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc gaattcgccg
     1861 ttcgcccgcc agatgtagat accagtgaga acaaaggaga tcacaattac cccaacacca
     1921 atcggaatac cgcgtgtgac gctggtgttc ggattcagcg gagtgcccag ccagccgggc
     1981 gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat cgacaggatg
     2041 gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc ttctatccgc
     2101 tgataaatcg taccattcat cacagattct ccagaggtaa tgtagggatt gttttaattc
     2161 ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt aggccggata
     2221 aggcgtttac gccgcatccg gcaatcaacg cctgatgcgt cgctgtcgcg tcttatcagg
     2281 cctacagccg ttgccgaatg taggccgggt aaggcgttat acgccgcatc cggcaatcaa
     2341 cgcctgatgc gtcgctgtcg cgtcttatca ggcctacagc cgttgccgaa tgtaggccgg
     2401 ataaggcgtt tacgccgcat ccggcaatca atgcctgatg cgtcgctgtc gcgtctatca
     2461 ggcctacagc cgttgccgaa tgtaggccgg ataaggcgtt tacgccgcat ccggcaatta
     2521 atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg actcgcatcc
     2581 ggcaattgtg ggttacgatg gcatcgcgat atcctgcttc tcttcaagca gcttctcgac
     2641 tacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc ccgccgcaat
     2701 tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg agtcggtcca
     2761 gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt tgcggacttc
     2821 tgcgtacagt tctggtgacg gttcctcccc atgattaagc gtgacgtagg cgtagatcgc
     2881 ctgaccttta atattgtgcg gaataccgac gacagcggct tcggcgattt tcggatgcgc
     2941 caccagcgcc gactcaatct ccgccgttcc cagacggtga cccgagacgt tcagcacgtc
     3001 gtccacgcgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc cgtcgccgct
     3061 gaaatacata tttttgaagg tggagaagta ggtctgctca aaacgttcgt gatcgccaaa
     3121 cagcgtacgc gcctgacccg gccaggagtc ggtgatcacc agactgcctt cggtagcgcc
     3181 ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga acggacgtgt
     3241 tgccgaaccg gcttttagct cggtagcgcc aggcagcggc gtgatcatga aaccgccagt
     3301 ttcggtctgc caccaggtgt cgaccaccgg gcatttctca ttaccgattt ttttccagta
     3361 ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc gcagtgacga
     3421 acggtcggtg ccttcaatcg ctttatcgcc ttccgccatc agcgcgcgaa tcgccgtggg
     3481 cgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac gggcaggcgt
     3541 cggccagttc ggtacgcctt caaacatcag cgtggtcgcg ccgcaggcca acgggccgta
     3601 cagcaaataa ctgtgtccgg taacccaacc cacatcagcg gtgcaccagt agatatcgcc
     3661 cggatgataa tcaaagacat atttaaaggt cagcgccgca tacaccagat aaccgccggt
     3721 agtgtgcagc acgcctttcg gcttcccggt agaaccggag gtgtagagaa taaacagcgg
     3781 atcttcggcg ttcatctctt ccgcctggtg ctgatcgctg gcttgctcaa tcaggtcgtg
     3841 ccaccacaga tcgcgccctt cctgccagtc aattttcccg ccagtacgct tcagtaccac
     3901 cacatgttca atgctgggga cgttcgggtt tttcagcgcg tcatcaacgt ttttcttcag
     3961 cggaatacta cgcccggcac gcacgccttc gtcggaagtg atcaccagtc gtgagttaga
     4021 atcgataatg cgtccggcaa cggcttccgg cgagaagccg ccgaaaatta ccgaatgcac
     4081 cgcgccaatg cgggcgcagg ccagcatcgc gaccgcggct tccggcacca tcggcatata
     4141 aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg cgaagcggca
     4201 gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt cgtcgccttc
     4261 ccagatgatg gcggtacgat cgccgttttc ttgcagatgg cggtcaaggc agtttgccgc
     4321 cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg caaaggaggt
     4381 gtttttcacc ttctggtaag gtttgatcca gtcgagaatt tttccctgtt cgccccagaa
     4441 ggtatctggt gcgttaatag attgttgata catcgcctcg tactgctgag ggtttatcag
     4501 gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttgactca tgcttttgtt
     4561 ctccttgtag gatgttaata atatgtggca taagcgttaa atgtaggggt attggcagtt
     4621 ttgtttagta tttgggcgac agatcacgca aaagtagaat tgtgcaaata aacggcaggg
     4681 taatttttga aggtcagcaa caaaagttga ttaattcttt gaggaacatg cagttatgca
     4741 tgctgttgaa aaagaggaag atactgacta actctaaagt ggtattttac atgcacttac
     4801 aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg cgagcaatgt
     4861 catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca ttgcaacaat
     4921 ggcgcaattc ggatgaagcc ctatgacaag gataaaaata aacgcacgcc gtatcttcag
     4981 cttattgatt ccttttttct ttttcacttc tgttcacgct gaacaaacgg ctactcccgc
     5041 aaaacctgta actgtggaag cgaagaatga aacctttgcc ccgcaacatc ccgatcaata
     5101 tctctcctgg aaagccacct cggaacagtc agagcgtgtt gacgccctgg cggaagatcc
     5161 acggctggtg atcctgtggg cggggtatcc cttctcgcgc gattacaaca agccgcgtgg
     5221 acatgccttt gctgtgaccg atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc
     5281 tgaagatggt ccgctaccga tggcatgctg gagttgtaaa agcccggatg tggcgcgtct
     5341 gatccagaaa gacggcgaag atggctactt ccacggaaaa tgggcgcgcg gcggcccgga
     5401 aatcgtcaac aacttaggtt gtgccgattg ccataacacc gcctcaccag agttcgccaa
     5461 aggcaaaccg gagttaaccc tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg
     5521 taaacctttt gagaaagccg gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca
     5581 tgtggagtat tacttcgacg gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat
     5641 gaaagtcgaa aatatggagc agtattacga caaaattgcc ttctctgact ggactaactc
     5701 cctgtcgaaa acgccaatgc tgaaagcgca gcacccggaa tatgaaacct ggacagcggg
     5761 cattcatggc aaaaacaacg tgacctgtat cgattgccat atgccaaaag tgcagaacgc
     5821 cgaaggcaaa ctctacaccg accataaaat tggtaatccg tttgataact tcgcccagac
     5881 ttgtgcgaac tgccataccc aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa
     5941 gcagtcgatt aacgacctga aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga
     6001 agcgaaagcg gcgctggatg caggcgcgac ggaagccgaa atgaagccga ttcaggacga
     6061 tatccgtcat gcccagtggc gttgggatct ggcgatcgct tcccacggca ttcatatgca
     6121 cgcgccggaa gaaggtctga ggatgctcgg tacggcgatg gataaagcgg cggatgcacg
     6181 caccaaactg gcgcgcctgc tggcgaccaa aggcatcacc catgaaatcc agatcccgga
     6241 tatctcgacc aaagagaaag cccagcaggc cattggcctg aacatggaac aaatcaaggc
     6301 cgagaagcag gacttcatca aaacggtgat cccgcagtgg gaagagcagg aacgtaaaaa
     6361 cggtctgtta agccaataac cccgttccgc ctcgcaaggg gcggaaaaca caatggagtg
     6421 aatatgagcg tattacgttc gttattaact gccggggtgc tggcgtcggg cctgttgtgg
     6481 agcctgaacg gaattaccgc tacccctgcg gcgcaggcat ctggtgatcg ctacgaagtt
     6541 accctgcagc gtaacccgga tgccgcctgt ctggactgcc ataaaccaga taccgaaggt
     6601 atgcatggca aacatgcttc cgttatcaac ccgaataaca aactgccggt cacctgcacc
     6661 aactgtcacg gccagccatc accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt
     6721 aacgagccga tgtacaaggt tggagagcag aacagcgtct gtatgtcctg tcatctgccg
     6781 gaacagttgc aaaaagcgtt ctggccacac gatgtccacg taaccaaagt ggcgtgcgcc
     6841 agctgccatt ccctgcatcc acagcaagat acgatgcaga cgttaagtga caaaggacgg
     6901 attaagattt gcgtcgattg ccacagcgat cagcgtacca atccgaactt taacccagcg
     6961 tctgttccgt tgcttaagga gcagccatga cctggtctcg tcgccagttt ctcaccggcg
     7021 tcggcgtgct ggcagccgtc agcggcaccg caggacgcgt ggtggcgaaa acgttgaata
     7081 tcaatggggt gcgttacggc atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct
     7141 gtatggatgc ttgtcgggaa gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta
     7201 ttcgtagcga gccgcagggc gaatttcctg atgtgaaata tcgctttttc cgtaagtctt
     7261 gccagcactg cgatcatgcg ccgtgcgttg acgtctgccc gaccggtgcg tcttttcgcg
     7321 acgctgccag cgggattgtc gatgttaatc cggatctctg cgtcggttgt cagtactgca
     7381 tcgccgcctg tccgtaccgc gtgcgcttta tccatccggt cacgaagaca gcggacaaat
     7441 gcgatttctg ccgtaagacc aatttgcagg ccggtaagct gcccgcgtgc gttgaggctt
     7501 gcccgacgaa ggcgctgacg tttggcaatc tggacgatcc caacagtgag atttcgcaac
     7561 tgctgcgcca gaagcccact taccgctaca agctggcgct gggaaccaaa ccgaagctgt
     7621 accgcgtacc gtttaaatac ggggaggtga gccaatgacg cagacttccg catttcattt
     7681 tgaatcgctg gtgtgggact ggccgattgc catctacctg tttttgattg gtatttctgc
     7741 tggtctggtg acgctggccg tgatgttacg tcgcgtctac ccgcaggcgg gcggtgcaga
     7801 cagcacgttg ctgcgcacca cgctgattgt cgggccgggc gcggtgatcc tcggtctgtt
     7861 gatcctcgtc ttccacctga caagaccgtg gaccttctgg aagctgatgt tccactacag
     7921 ttgtacctcg gtgatgtcga tgggggtgat gctttttcag ttttacatgg tggtgctggt
     7981 gctgtggctg gcgaaaatct ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc
     8041 gaagctgggg atcgtgcaaa aggttctgag cctgctgacg cccgttcatc gcggactgga
     8101 aacgttgatg ctggtgctgg cggtgttgtt gggggcttat accggctttc tgctgtcggc
     8161 gctgaaatcg tatccgttcc ttaataaccc gatcctgccg gtgctgttcc tcttctccgg
     8221 catctcgtcc ggtgcggcgg tggcgctgat cgccatggcg atatgtcagc gcaaaaatcc
     8281 gcattccacg gaagcgcact ttgtacaccg tatggagatc cctgtggtat ggggcgaaat
     8341 cttcctgctg gtggcgtttt ttgtcggact ggccctgggc gatgacggta aagtgcgtgc
     8401 gctggtggcg gcattgggcg gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg
     8461 gctggggctg attgtgccaa tgttgctcaa accgtgggtc aatcgcagtt ccggcattcc
     8521 tgctgtgctg gcggcgtgtg gggccagcct ggtcggcgtg ttgatgctgc gttttttcat
     8581 tctctacgcc gggcagttaa cggtggcgta agccagaaca gaggtggttt ctggacgtat
     8641 tccttcctga agtcggtttt ctggcgttgt tgttaagtct cggggtcaac gtgttgaccc
     8701 cgttgacggc cttcgcggga gtgcggttgc gctggcctgc catgatgcga ctcacttgca
     8761 tcggcattct ggcgcagttc gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt
     8821 ttctcatcag cgatttctcg gtcatttatg tcgcccaaca tagctacagc ctgctgtcgt
     8881 gggaactcaa actggcggcg gtgtggggcg gtcatgaagg ttcgctgctg ctttgggtgc
     8941 tgttgctttc cgcctggagc gcgctgtttg cctggcatta tcggcagcaa accgatccgc
     9001 tatttccgct gacgctagcc gttttatctc tcatgctcgc cgcactgcta ctgtttgtag
     9061 tgctgtggtc cgatcccttc gtgcggatat ttccaccagc aatcgaaggc cgcgatctca
     9121 atccgatgct gcaacatccc ggtcttatct tccatccgcc gctgctttat ctcgggtatg
     9181 gcggtttgat ggtggcggcg agcgtggcgc tggcgagttt actgcgcagc gagtttgatg
     9241 gtgcctgcgc ccgaatttgc tggcgctggg cactaccagg ctggggggca ttaacggcgg
     9301 ggatcatcct cggttcctgg tgggcctatt gcgaactggg ctggggcggc tggtggttct
     9361 gggatccggt ggaaaacgcc tctttattac cctggctttc tgccactgcg ctgctgcaca
     9421 gtttgtccct gacacgccag cgggggattt tccgccactg gtcgctgtta ctggcgatag
     9481 ttactctgat gctgtcgctg ctgggtacct taattgtccg ttctggcatt ctggtttcgg
     9541 ttcatgcgtt cgcgctggat aacgtccgcg ccgtgccgtt gttcagcctg tttgcactga
     9601 ttagccttgc gtctctggct ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc
     9661 atttttcggg gttatcgcgg gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag
     9721 tgctcctgat cgtgctggtg ggaacgcttt atccgatgat ttacggcctg ctgggctggg
     9781 gacgcctctc cgttggcgcg ccgtatttta accgcgcgac gttaccgttt ggtctgttga
     9841 tgctggtggt gattgtgctg gcgacgtttg tctctggcaa acgcgtgcag cttccggcgc
     9901 tgctggcgca tgcgggcgtg ctgttatttg ccgcggggat cgtggtctcc agcgtcagcc
     9961 gtcaggagat cagcctgaat ttacagccgg gtcagcaggt gacgctggca ggatacacct
    10021 tccgttttga gcgtctcgat ctgcaagcca gaggcaatta caccagcgaa aaagcgatag
    10081 tggcactgtt tgaccatcag caacgcattg gtgaactgac gccggagcgg cgtttttatg
    10141 aagcacgccg tcagcaaatg atggaaccgt caattcgctg gaacggcatc catgactggt
    10201 atgcggtcat gggggagaaa actgggccgg atcgttacgc ttttcgtttg tatgtacaaa
    10261 gcggtgtgcg ctggatctgg gggggaggat tgttgatgat tgcgggcgca ttgttaagcg
    10321 gatggcgggg gaggaagcgc gatgaataaa ggacttctca cgctactgct gttatttacc
    10381 tgttttgcgc gcgctcaggt tgtagacacc tggcaattcg ccaatccgca acaacagcaa
    10441 caggcgttaa atattgccag ccagttacgt tgtccgcagt gccaaaatca gaacttactg
    10501 gaatccaatg cgccggtggc agtcagtatg cgccatcagg tttacagcat ggtggaagag
    10561 gggaaaagtg aagtcgaaat cattggctgg atgaccgaac gctacggaga ttttgttcgc
    10621 tataacccac cgttaacggg tcagacgctg gtgttgtggg cgctgccagt ggtgttgtta
    10681 ctgctgatga tactgatcct ctggcgggta agggcgaagc gatgaaacag cctcaaatac
    10741 cggtgaaaat gctgacaaca ctcacgattt tgatggtatt tctctgtgtc ggcagttatc
    10801 tgttaagtcc aaaatggcag gcggtacgtg cggagtatca gcgtcagcgc gatccgctac
    10861 atcagtttgc cagccagcaa accccgaaag cgcagcttca ggcattgcag gataaaatcc
    10921 gcactaatcc acaaaacagc gaacaatggg cgttactggg cgagtattat ctgtggcaaa
    10981 acgattacag caattcgctg ctggcttacc gtcaggcgtt gcaactacgt ggtgagaacg
    11041 ctgaactgta tgcggcgctg gcgacggtgc tttattacca ggccagccag catatgaccg
    11101 cccagactcg cgcaatgatc gacaaagccc tcgcgctgga ctctaacgaa atcaccgctc
    11161 tgatgctgct ggcttccgat gcgtttatgc aggcgaatta cgcgcaagcc atcgaactat
    11221 ggcaaaaagt gatggatctc aactcacccc ggatcaaccg gacacagctg gtcgagtcga
    11281 ttaatatggc gaaattgttg cagcggagat cagattaatc atctggtgat gaagattatg
    11341 ttattgagct gcataaaaaa taatcgaatg aacatatgcc caaaataatc actaatcagt
    11401 attattgcag attaaacaaa taaaaatctt tctataacaa atggttattc attaatcctg
    11461 ccataaactc gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg
    11521 caacataccg cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa
    11581 cacaattcat gccctggcag tatgtcacgt tctcacgttt ctgaacgggg aacggcgctc
    11641 cattgaggaa gttattctga tgaaaaatat aaaattcagc ctggcctggc agattctgtt
    11701 tgctatggtg ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga
    11761 ctggctggtc gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgattaaaat
    11821 gattgttgtg ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc
    11881 caaacagctc gggcgtattg gcgcgaaaac cattatctac ttcgaggtga tcaccaccgt
    11941 cgccatcatt ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat
    12001 gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag
    12061 cagttcccac ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca ttgtggcgtc
    12121 gatggcgaaa ggcgaaatgc tgccgatcat cttcttctcg gtgctgtttg gtctggggct
    12181 ttcctccctg cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga
    12241 aaccatgttt aaagtgactc acatggtgat gcgttatgcg ccggtgggtg tgtttgcgct
    12301 gattgcggtg acggtggcta actttggttt ctcgtctctg tggccactgg cgaaactggt
    12361 gctgctggtg catttcgcca ttctgttctt cgcgctggtc gtgctgggaa ttgtggcgcg
    12421 cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc
    12481 gtactccact gccagttctg aaagcgtgct gccgcgaatt attgagaaga tggaagccta
    12541 cggagcaccg gcgtcgatca ccagtttcgt ggtgccgacc ggttactctt ttaaccttga
    12601 tggttcgacg ctgtatcaaa gtattgccgc tattttcatc gcgcagttgt atggcattga
    12661 cctgtccatc tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat
    12721 tgctggcgtg ccgggcgtgt cgtttgtggt gttgctggca acgctgggca gcgtagggat
    12781 cccgctggaa ggtctggcgt ttattgccgg tgttgaccgt atcctcgaca tggcgcgtac
    12841 tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa
    12901 atttgaccgt aagaaagcgc tggcttatga gcgcgaagtg ctgggcaaat ttgataaaac
    12961 tgcggatcaa taattgaaga ttgccgggga tatccacccg gcaatgtgtg gatgcctgat
    13021 gcgacgcttg ccgcgtctta tcaggcctac gctagactgc gcaatagctt gatttagggt
    13081 gattttgtag gtcggataag gcgtttacgc cgcatccgac atcaatatct gatgcgacgc
    13141 ttaccgcgtc ttatcaggcc tacgccagac tgcgcaatag cctgatttag cgtgattttg
    13201 taggtcggat aaggcgttta cgccgcatcc gacatcaatg cctgatgcga cgcttgtcgc
    13261 gtcttatcag gcctacctta accgttggtt aatttctcaa actcttcaca accggtatca
    13321 aacccttcca tacagctcag gttcagccag tgcagcgcct tttgcttgtt cttctcgata
    13381 aaaccttctt caccgtttaa gaacatcatt cccgcccagt actcggaata accggttcgg
    13441 gaaatcgcag agctgcgttt gaaataccag gttgccttgt catcgtcggc cttaatgccc
    13501 acgccgttgg cgtaaatcaa accaagcagc atttgggcat cgacggcaga gtcgttctcc
    13561 agatcttccg aagcgttttc taacagcgaa atcgcctttg gataatccgg tttacccgct
    13621 tgcgtattta ccagaatatg cgccagcgtt acttcacctt ctttactccc cgcttgcgct
    13681 gctttctctg caagaacttt tgcttgtgga tagtccagac tgaccggatt ggtgattttg
    13741 atctgcgcca acagcgcgca ggcatcagca tcaccgctgt cggcggcttt ctgtgcccaa
    13801 tattcggctt tgctcaaatc gccggagcta aaccagctgt cggcaagaaa atattgtgcg
    13861 cgtcggtccc cggcctctgc tgcctttaaa tactggctgc caggctcgga gtcgttcgcg
    13921 tgggcaaaga atgtcaaaaa caacatcaat gcgataattt ttttcatttt gataatacga
    13981 ttgggtaggg agtaaccagt ataaagagtt cgaatggata aaaaaacagc ctccggaagg
    14041 aggctgtaga aaggactgta ttacgccagt gccgcttcgc gcaggcgagt tttcaacttg
    14101 ttgtactcgt caatcacgta ctgctcggcg gcgcgctgat cggcgatcga ctcgacgcgc
    14161 acggcgcagt atttgtattc cggcgttttc gtaatcgggc ttaagttttc ggtaaccagc
    14221 tcgttacagg caccaatcca ccactggtag gtcatataaa tcgccccttt gttcggacga
    14281 tcgctgacct gcgcacgggt gataattttg cctttacgcg agtgcaccca aaccaatgcc
    14341 tcatcttcaa tacccagacg tttggcgtct tcggtattga tttgtgcgta gccaggttca
    14401 tcagccagcg ccgccagtgc cgcacagtta ccggtcatcg aacggcaaga gtagtgacca
    14461 acttcacgca ccgttgacag taccatcgga tactcgtcgg tgagtttgtc gattggcgct
    14521 acccagtcgc aggtgaagaa ctgcgccaga ccgttcgggg tatcaaactt ctctttaaac
    14581 agataagaag tcccctgatc ggcatctgaa gtatcgcggc aaggccactg aatgaagccc
    14641 agttcgccca ttttctcgta agtcgcaccg tagaaatccg ggcacagatg acgcaactca
    14701 tcccagatct cctgggtgtt gttgtagtgc atcggataac ccatacgggt ggcgatttca
    14761 ctgataatct gccagtccgt tttcagatcc cacttcggtt caaccgcttt gaagaaacgc
    14821 tggaagccac ggtcagccgc agtaaacacg ccttcatgct cgccccatga cgttgacggt
    14881 aaaataacat ccgccgccga cgcggttttg gtcataaaga tgtcctgaac gataaccagt
    14941 tccagatctt caaaggcttt acgtactgcc gacagctccg cgtcagtttg cagcggatct
    15001 tcgcccataa tgtacgcggc acgcacttcg ccatgcgctg cgcggtgcgg cagctcgctg
    15061 atgcgatagc cggtatgcgc tggcaggctt tccacgcccc aggctttggc gaatttctcg
    15121 cggttagccg gatctttcac gtactggtat cccggatacg tatccggcag cgcgcccata
    15181 tcgcaggcac cctgcacgtt gttctgacca cgaaccgggt ttacacccgc atgcggctta
    15241 ccgaggttac cggtcagcat cgcgaggctg gtcagagaac gcacggtttc cacgccctgg
    15301 tagaactggg ttacacccat gccccacagg atggcggcgc ttttcgcctg ggcatacatc
    15361 cgtgccgcct gacgaatctc actggcgctg acgccggtga tatcttcaac cgactccggc
    15421 gtgtagcctt caacgatttt acgatactct tcaaagcctt ctgtacggga agcgacgaat
    15481 gctttgtcgt acagattttc ttcaataatg acatggccca tcgcattcaa cagcgcgatg
    15541 ttcgagccgt ttttcagtgc aatgtgcatg tccgcaatgc gcgcggtttc aattttgcgc
    15601 ggatcgcaga cgataatttt cgccccgtta cgtttagcgt taattacgtg attcgccacg
    15661 attgggtggg aatccgccgg gttgtacccg aaaacgaaca ctaaatcggt attatcaatt
    15721 tcgttaatag cattgctcat tgcgccatta ccgaccgatt ggtgcagacc tgcaaccgat
    15781 gggccgtgtc agacacgagc gcagcagtca acgttattgg taccaataac ggcgcgcgca
    15841 aatttttgca ttacatagtt ggtttcgtta cccgtaccac gcgaggagcc ggtcgtctgg
    15901 atggcatccg gaccgtactt ctctttgatg gcgctcaggc gctcggcaac gtaattcagt
    15961 gcctcatccc aggaaacagg ttcgagtttg ccgccacgct ggcgacggat catgggggtt
    16021 ttcaggcgcg gggtcaggat ctgggtatcg ttaatgaaat cccagccata ataacccttc
    16081 agacacaggg taccctggtt ggttttcccc tgcgctgcct ccgcccggac gattttgccg
    16141 ttatcgacga ccaggttgat tttgcaacct gatgcgcaat aggggcaaac cgtgacgact
    16201 tttttcatcg gtctcgctcc agttaatcaa atcacgcata cgcgctctcg actacagtat
    16261 gcatctttta tgccacattt tatttggggt cattccctga tattacgggc actatttatt
    16321 caaaactctg acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg aaataccgcg
    16381 tgacagccat cacgcggcag acattttatt ttttctcgac gacgggactt gcctgatacc
    16441 cgccgcccag cgatttcatc aactgaatgc tttggatcac ccggcggctg tccagcatca
    16501 gtaatgacat ctcctcggca agcactggca accgggcttc ggtggcctgt aagcggctgg
    16561 ttaagccgcg ttgataggcg gcctcggcag cgcgctgggt gaagcgcgtg gcttccacgc
    16621 gttcagcctg catctctcgc tcgtcgttga gcgtttgcag acgcgtgccg ttgacggcaa
    16681 cgtcgcgcac cgcattcagc actgactggt tgtaacgttc aatcatcatg ttgctggcgg
    16741 cgcgagtgcc ttcgagattg gcattcaacc gtccaccgtc aaacagcggc aatttcagac
    16801 ccgggataaa gttgaactgg cgactggttt ttttgaataa ggtatccagg tggatagaat
    16861 ccaggccgaa aaacgctttg atatcaaagc tcggatagaa cagcgcccgc gcggaatcca
    16921 cctgatctaa tgacgcctga acataccagc gcatagcttg cagatccggg cgtctggcga
    16981 gcaactcata agagagtgtc gccggaatac cggtctggac tcgtggtaat gccaccggtt
    17041 tgatctccgg catatcgctg gctcccgcgc caatcaatgc gcgcagagat tctctcgttt
    17101 cggtgatttg ccctttgacg gcagcaattt gtttatcgac cgccagaatc tgtgcccgcg
    17161 cgccgtggaa aggcacttgc gcttccagac cgtgcgccac tttactctgg tgcgctttta
    17221 ccgcgtaatc aatcacatcg cgagtttgtt ctaacagatc gagcatctga tagctggcct
    17281 gcatactgta ataaagctgc gctacgccag tggtcagcga tagctctact gctgcggttt
    17341 ctgccagcgc ggcattatgc gcgccaatgg cggcggcaac cgctgagcga tgcacacccc
    17401 acaaatcaag atccagtccg gcaaacaaac cgactgtggc ttccgtatag tacggcccgt
    17461 ccatccccag tgcgggcgca tccatcgcat aagggcttaa aaagccgttc gccgagacgc
    17521 gttggcggtt aagcatccct aacgctgcca cctgcaactg tgaaccggta tctaacaaat
    17581 cggcctggga ctgcgctttt tcttcccgca gtttcgcttc ggcgagggtg tgtgaaccac
    17641 ttagcgtccg ttggatcagg gcatccaact gcgggtcatt gagttgtttc caccactgcg
    17701 cctgcggcca gccggagctg gcaagatgaa tatcatctgc cagtttgatt tgttcaggtt
    17761 tgagctgttg atgaggcgca gaatccttgc gtaccagggc acagccggaa atcagcgtcg
    17821 tgctgccgag aatgctgcac aacagcagac gtgaaagttg acgattgatc attgcgtggc
    17881 tccttgtgcc tgttccgtgg cgggcgttaa tgccggggct gtccagtctg gcaagcgggc
    17941 gaaaagctgg cagacgtgtt gctcctgttt taataaggtg ggcaggatgg cctgcgaggc
    18001 gggtgtttct tcaagcgtta tttgaggaga acggctgagt gcggttgcca gaccggcggc
    18061 gtatttctcc agtgcgtcgg caaactgacc tgctctgtcc ggctgtagtg cgttatccag
    18121 cgcctgcgcc gagttccagg ttgcatccca ggcgtgaaga atatcgcggc cctgacgaat
    18181 aaccgtttgc gaacgttcaa tcagtaaagc gcgttcttcg ctgtccagtt gacgctccaa
    18241 cgccacgcgt tggcacatct cttcacaggc attaaacgcc gcatgcagac ctatacgaat
    18301 ttgcaaataa gtgcgcagag ctgtgacttc ctgctggcgt gggatccgca ttactttact
    18361 taacataccc agcgcgccag ccagtttttg cggcagggtg cgcgcttcac tttcaggcca
    18421 gacaaaggtg taaatcaccg cggacaccac ggtaccaatg atgataccca gggcgcgatc
    18481 gcgaatttcc accaggtcgt acaccgggcc aaaaacattt tcgagcgtgg cgagcgcgaa
    18541 ggtaaccacc atctgtgtgc cgatataaga agagcgttca gagctggtgg caatccatgc
    18601 gcccaacagg aaaatcggtg ccagcacaaa cagcaattcg acaatattgt ccagccaggg
    18661 catgaccagt agcgtgaata acagcgccag aattgcaccg cagaaggcac cgccaaaacg
    18721 cagcgccatt ttctgatacg acgaaccaac attcgggtta gcgacgatca cgcaggtcag
    18781 catacaggtg tgaatgcctt cccagtccgc gccgctgtag aaggtgtaac agatcaaaca
    18841 ggcgagcagc gtttttaccg cgtagcgcat atagtctgga ttggtaaaag catcggcgac
    18901 cattgatggc ggtttggtgg cgggcgttgg cggtgtattc gggtccatct gacccagttg
    18961 caacaatgtc tggcagatat tctccaggtt acattcccgt gccgccatcg cttcactttc
    19021 agtgatccgc cagtcgcttt gccagcactg accttcggca atggcatgct gcagtttgtt
    19081 gatttctgtg gctaattttt gccgaaattc aataattgcc tgagaatcag caaaagaggt
    19141 gggatcgtag cgattcagcg tcgagtaaat gtaggttacc gttgccacgc agctttgcca
    19201 ccatgcgctc tgagttcgcc agttggcatc gtccgcgagg caaaagacat tgagtttttg
    19261 cagcgccagc gcctctcttt caatccgcgt ttcgggtagc ggtgcgaggc tgtccgtcag
    19321 gtgactaatg gcatcatcaa gccgatcatt aagcgcttga tgcatttgcg taatggtgcg
    19381 gctgggaaac cacagcacgc cgattaacgt catcagcaag gttggataga ggccaacaac
    19441 gatacaccac agggttaagc gcacgaccac ttccggatag tcgagcatgg cggggaaggt
    19501 ttgcccgtaa atagcgacaa tggcgacggc gaaaaagacc agccccaacc gatgggtgcg
    19561 catcaaaaac atacagccca tcaggatcgg tccggcaatg atcaatcgga tcaacggttc
    19621 gccatatgac catttgtaga tcaaaaacag gctggcgatt tccagcatcg tggcgaccac
    19681 aaacaagatc gccacaaatt tggtgtaaaa cgcgttcgac tgaataccgt aaaacagcac
    19741 tgccagtgat aacgccacaa aagggatctc aaaagtcatc gagatcagaa tcaccagcag
    19801 gcagcctacc cagagttgca tagtctgcgg cacgcggcct ggtcgccgct cgcttaactc
    19861 ttcatgaaag aacgccagca gcctgaccac cggtaatggc agggagttga gcgcgctcat
    19921 tattgcggct caagattagc gactgccgaa gcgccgatgc ggaacatttc cgggtcaggt
    19981 ttatcaacca tgattttgac cggaaaacgc tgggcaacgc ggacccagtt aatagaacga
    20041 gacactttcg gcaggccgcc cagcaccagg ccgccgtcat ccggtagcac gccgtagcca
    20101 atcgaatcca ctttaccctc gaaggttttg ccgctgtcgc tcatcaggcg aatcgttgcg
    20161 ggtgtacctg agcgaatatt tttcagatca gtttcgcgga aattggcgat cacgtaccag
    20221 tgacgagtgt cgattagggt gaaaataggg cgcatggcag aagcaaattg cccgacggag
    20281 gttttgaggg aaatgacccg gccatcaaac ggcgcgcgaa cggtcgccat ttccagatgc
    20341 agtttggtca gggcaatatc cgcttcgacc gccgcacgtt gcgccaccag cgcatcgacg
    20401 ccgctgacgg cgctggcggc tgactgcgcc tgtaacaata cggcattaag atccgcttct
    20461 gcagcgcgct gcgcagttct tgcacggtca acgtcttccg ctgagacaaa accttctttc
    20521 agtaatggct cggtgcggcg taatgtatct gtggcctgtt tcgcggcggc acgggctttt
    20581 tctaccgtgg cattaaccga gtcggcacca aactgttgcg cgtcaacgct acgctgggtg
    20641 agcataattt gcttatccag cgccgcgagg gaggcttcgg ctttcgccag attggcttcg
    20701 tacgggcgcg ggtcgatgcg gaacagcaaa tcgccctgtt tcaccgcctg gttgtcggtg
    20761 accgccagtt ctacaatgcg accgctgact tccggcacca catcaatggt atctgctgac
    20821 gcgtaggcgt cattagttga tggcgcactg tctacgcgcc agataacgaa aacaagggca
    20881 accagcgcca acgcaaccac taacagagca gggaatttac tgcgaggagc ttttttcggc
    20941 gtactttcca tgagcgcgtc tcaaataaat tagaaaaatg ccagccagag cagcatggcg
    21001 tagagagcaa aaagggcggt ataaataatt ccgacaaatg ccagattgat atttgcccgc
    21061 tgaataattc gcctcgtgat gagcgtcaaa ataaggctgg caatggcgca gaaaaaccag
    21121 ctgggataat aagcgccgat caccgggatt gccggagata atgagcatcc cgaaaccatg
    21181 aagacgggaa ttatccaggc tgtttttttg agttgcatcg ccatgcgaga gagaactgtt
    21241 ggcatattaa atacatcctg tctgctgcga acaatatctg tagtgaatat caatatccat
    21301 tgaaggcgtc agtatggcta ttttctcgat gaataaaaat agctttattc agccaccgca
    21361 ttcataactt atatatttta tgtattaatt tgctgtatat attttttaaa taaatgcttt
    21421 tgggggctta agggccccca aaagtgagtg gcattaattt ggggttacga tattcaccca
    21481 caaatcgaaa ttatccagac aggcaataat ttcttccagt ttcgcaccat tgccaataat
    21541 tttggctttc ttcgctttta ccagatccgc cattttggct tgtccggtca gcacggcgtg
    21601 aagatcttca cggctgatgt agaaagaggc gttggcttca ggttgcagcg ttttacggta
    21661 gttaagcacg ctatcgttca gcgtcaggtt gaggttatcg ccgttgctca tattgaagtt
    21721 caggctgata ttcttacctg cggctttcgc actatcgagg cgaacggaca taaagtcgaa
    21781 cagcatttcg accgacattc cgcgaatggt gtccggggaa ccggtggtgc cgtggctgaa
    21841 tttatgcacc ccttcgcgca gctctttcgc gccggtcagg tagaaaccgc gccaggtggc
    21901 ggactcggcc tgatagccca gctgttcaaa gttattcgct tgcagattct tcgcgacctg
    21961 gtcaccaggg ttggcggcaa tcacctgttt cagcagttct gccgaccagc ggtaatcacc
    22021 ttgcttgttc gcttcttgcg ccaggttgat gacacgggca gaaccgccca gcgcctgcac
    22081 gtaacgttta cccatctcca cctgaccata cggatgcagg ttagccgggt taccgtcgta
    22141 atagccaaga tagaagttat acaccgcgcg ggcgttgtgg ctgacagaac cgtaatagcc
    22201 gcggctggcc cagttattgg caagcgcagg cggtagctta atcatgtcgc cgatttcatt
    22261 catggtgtag ccctggtttg ccaggtgcag ggtctggtcg tgaatgtact tgatagtatc
    22321 gcgatattta ccaatataat cattgatatg cttattgccc cagaccggcc aggtgtgcgg
    22381 cataaacagt acttccgcgt cgttacccca catatccagc gtttcgttca ggtactcggt
    22441 ccacttgctg gtatcgcggg ttttcgcgcc gcgcagagtg tagaagttgt gcagggtatg
    22501 cgtggcgttc tcggcggtac acagggcttt cagggccgga atatagaagt gcatttcggc
    22561 tggcgcttcg ctacccggag tcatcaggaa gtcaaactcc aggccgtcga taatcatctt
    22621 ctcgccagtt ctgacgatag ttttagtcgg tgcaataatg ctcgggtcgc cggttgccag
    22681 cgtcacgccc aggccattac ccacgttgcc ttgcgcgttg tgcggcagta acagaccgta
    22741 agagtagagc gcacggcggc tcataatatt acccgccagc acgttttcgc tgatggcttc
    22801 gtccataaag cctgccggag cgatcacctg cactttgccg gatttaacat ccgcttcaga
    22861 gataatgcct ttcacaccgc cataatggtc ggcgtggctg tgggtgtaaa taacagcaac
    22921 aatcggtttt tgcggacgat gttggaagta aaggtcaagt gcggcttttg cggcaggcgg
    22981 cgtcaccagt gggtcgataa caataatgcc tttctcgccc tcaacgaatg taatgttaga
    23041 gatatcctgg ccgcgcacct gatacatttt atcggtgact ttgaacaggc cagaaatgcc
    23101 gttgatctgc gactgacgcc acaggctggg gtttacggtt tccggcgcag cggcattaat
    23161 atcaaatttg taatcattcg cgcggtagta aactttgccg ttagcatcac gcagaatacc
    23221 ttcatccagc agcggggcga taaatccacg ctgggcatcg tcaaaatctt gacgatcgct
    23281 aaacggtaat gatttggcgt aattctgatt aatctgttgg gtatattgag tggcggcttt
    23341 cgcttcatct gttgcgtaag cggtatttac catcatgccg ctggcggcag ttaacgcaat
    23401 aacaatcctg ctcaaacgga ataaccgaga gttattcata taaatctcca tttatcattt
    23461 atgtcgtaaa tatgtaaccc atgcaaaata cgtcatttga agctggcgtt atcataattg
    23521 ggaaattgcg actttaataa gtagaagtgt gagctgaacg cgccatttta ttaggcattt
    23581 gtgctgaaga gggaa
//
LOCUS       DAYPHN010000065        23553 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000065 DAYPHN010000000
VERSION     DAYPHN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.065"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACG0BW_002444"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACG0BW_002444"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFK7259395.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRLLRPAILWNDGRCA
                     QECALLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLNNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACG0BW_002445"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACG0BW_002445"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFK7259396.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFHHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACG0BW_002446"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACG0BW_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7259397.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKETVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACG0BW_002447"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACG0BW_002447"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFK7259398.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSILLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACG0BW_002448"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACG0BW_002448"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFK7259399.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPTSTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACG0BW_002449"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACG0BW_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFK7259400.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACG0BW_002450"
     CDS             complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACG0BW_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFK7259401.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACG0BW_002451"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACG0BW_002451"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFK7259402.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPIWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACG0BW_002452"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACG0BW_002452"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFK7259403.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13021..13494)
                     /gene="ysaA"
                     /locus_tag="ACG0BW_002453"
     CDS             complement(13021..13494)
                     /gene="ysaA"
                     /locus_tag="ACG0BW_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFK7259404.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     TMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13596..14444)
                     /gene="yiaJ"
                     /locus_tag="ACG0BW_002454"
     CDS             complement(13596..14444)
                     /gene="yiaJ"
                     /locus_tag="ACG0BW_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFK7259405.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14646..15644
                     /gene="yiaK"
                     /locus_tag="ACG0BW_002455"
     CDS             14646..15644
                     /gene="yiaK"
                     /locus_tag="ACG0BW_002455"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFK7259406.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLDNGDIIPDAEAKRITTLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGAT
                     RDAKLQRIMDYITTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15655..16122
                     /gene="yiaL"
                     /locus_tag="ACG0BW_002456"
     CDS             15655..16122
                     /gene="yiaL"
                     /locus_tag="ACG0BW_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFK7259407.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTRPAEENRPEVHRRYIDIQYLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHHCENESFIKMTPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16240..16713
                     /gene="yiaM"
                     /locus_tag="ACG0BW_002457"
     CDS             16240..16713
                     /gene="yiaM"
                     /locus_tag="ACG0BW_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFK7259408.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16716..17993
                     /gene="yiaN"
                     /locus_tag="ACG0BW_002458"
     CDS             16716..17993
                     /gene="yiaN"
                     /locus_tag="ACG0BW_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFK7259409.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLEMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18006..18992
                     /gene="yiaO"
                     /locus_tag="ACG0BW_002459"
     CDS             18006..18992
                     /gene="yiaO"
                     /locus_tag="ACG0BW_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFK7259410.1"
                     /translation="MKLRSVTYALLIAGVAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKDAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            18996..20492
                     /gene="lyxK"
                     /locus_tag="ACG0BW_002460"
     CDS             18996..20492
                     /gene="lyxK"
                     /locus_tag="ACG0BW_002460"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFK7259411.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHTGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDQQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            20489..21151
                     /gene="ulaD"
                     /locus_tag="ACG0BW_002461"
     CDS             20489..21151
                     /gene="ulaD"
                     /locus_tag="ACG0BW_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFK7259412.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWCEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            21144..22004
                     /gene="sgbU"
                     /locus_tag="ACG0BW_002462"
     CDS             21144..22004
                     /gene="sgbU"
                     /locus_tag="ACG0BW_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFK7259413.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCSFDFVEMSVDETDER
                     LSRLDWNAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDKAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTLPVTG
                     QNPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            21998..22693
                     /gene="araD"
                     /locus_tag="ACG0BW_002463"
     CDS             21998..22693
                     /gene="araD"
                     /locus_tag="ACG0BW_002463"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFK7259414.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKGVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETLEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(22725..23540)
                     /locus_tag="ACG0BW_002464"
     CDS             complement(22725..23540)
                     /locus_tag="ACG0BW_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7259415.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     AIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADRQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         5752 a         6083 c         5994 g         5724 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttctcgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggcagt tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagag ctggccccac atcccccccc gtgcggtaat
      241 cgagttgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagcagccg
      541 cgattaaagc tgggacattg ttcaggcccg ttaatttcgc agcccaatcc agacacgacg
      601 ctgcactcag catcacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacggcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc tacaccaacc gcaccagctg
      781 cattatcacc accgcctgcg acaactggca ccgccgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtggca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taacaatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttaccggta atcacccgcg
     1141 attgcggaac tcgcgcttcc agcaaagcgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgtaataac cgttgctggg catccagtaa ggttgcaccg tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccagg gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc gccctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatggttta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttgtgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcctaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcacca tcttcaatca tgcgtgctgc aattttcagc gccaacgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccgccgg tggtgaaacc gcctgctttg agaatttcat acatcaccaa
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gtgcatcgcc
     1981 acggttagcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatacac cgtcgcagca tcgtaatcat attgatgttt
     2161 agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac gcaggtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccacccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgagttgcc gcccaactaa agacttctgg
     2401 atctgggttc gtcgcagcac ccgcaccgta gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaatc gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattatag tgatggaatg ctaacgggtt
     2821 agaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgaggaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct ctgtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc tacctacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgattaacc
     3481 ggggcgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactacag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc gggcaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacattgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccactct gaataatggt ctgaaagatg
     4141 ttccctcccg cctgctgaca ccgatcgatg tgaataaaaa caacatcaaa gaaacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gaggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttacccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgttt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcga ttttactgga
     4861 tattattcgc gatctgcaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggtcagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatat taggtattgc cggactcgtt ggtgccggac gtacagagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta attgtcagca agccatcgcc caggggattg cgatggtccc
     5341 cgaagacaga aagcgcgacg gtatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggcggtat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggta atcagcaaaa agcgatcctc gctcgctgcc tgttactcaa
     5581 cccgcgcatt ctcattcttg atgagcctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggttgaag tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gctcgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggctaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaatcggg caaagctatc
     6361 tacccgccag caccggcttc atcattggcg cgcttggctt aatggctttt gttggctggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccgacc tctaccgcag
     6481 ttgtcggtcg ccaggcatta acggcgatta tcgtcttagg cgcaatctgg ctgttgaacg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtattc gctattaacg
     6721 gattaatggt cgctatcgcc ggattaatcc tcagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgccgcatg tgtgattggc ggcaccagtc
     6841 tggcaggcgg cgtcgggagc gtggccggag cggtaatggg ggcattcatc atggcttcac
     6901 tggataacgg catgagcatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg taacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgcc gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggtaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcagggatta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctcccaccgc aaaccggaat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcacgg caaatgggct atcaggcggc aaaactattg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgga ttttggtccc gccagttcgc gtcattgaac ggcgctcaac
     7921 agattaccgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcgacg tagcatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgacagacaa atcggataag gctcgaggaa atgcaattac atatgtgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatctgctt tacgcagctg
     8521 tgcacgcgat ttacggaacg aagagtaagc cggatgcgta ttcaggttag taacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtacccc ttcactttac ccggcgcatt
     8641 agtacaacgt cctttcatgc atttcatgcc gaacagattg ttgttgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgct accatactgg tggggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatgagcc agttacgttc cgcagtaatg gccgcatttt ggctggtaat
     8941 gtaaggcatt acagtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 attttttaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggtttcccgc
     9601 ctttagcgaa caggggacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaac tagacacccg ctccggtaca ccaacattga tgatttccat
     9841 ccagaacgcc gtcgaaccgg tagcaagcct ggtccgtgaa tgcccgatat gggatggatt
     9901 accgctcaca ctggatgtca gcgccacttt tccggaggga gccgcagtcc gggattatta
     9961 cagccagcaa attgcgatcg tgaagaacgg tcaaataacg ttacaacccg ctgccaccag
    10021 caacggttta ctcctgctgg aacgggcgga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcagaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ctgggcgtca atgctttatg
    10261 gataagtgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcagtgaa gccgatctac ggacactggt tgatagcgcg catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccaca ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaaacgttgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa ccgatatcgg
    10681 cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaacgaccg cttctggtct gccagtgttc tataaaaaca aaacggatac
    10801 ccacgctaaa gtcatcgacg gctttacccc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgac accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggctaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg atttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 cctgttccgt gaagggggcg acaaagcagc agagttatta ttattagcgc caggcgcggt
    11281 acaaatcttt tatggtgatg aatcctcgcg tccgttcggc cccaccggtt cggatccgct
    11341 gcaaggtacg cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gctctaatca gcgttgcagg ataaagcacc
    11641 gctccctctt caacagagcg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcccgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaaccac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgttcg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 tccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 tggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 cgaagcgcgc ccgctgtgga atccgaatat cgtactgtgc atgagtcttt ccaagctggg
    12481 tctacctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ccgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 gattcataaa ccggaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttccca gggctggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg tgtgaagatt ttggctgaag agattgaacg
    12961 cgcctgggct gaaagccatt aatacgctat tccggcgttg agaaacgccg gaaaaacgtt
    13021 tcaaacaggc tgctgccgta gccgatgccg ctgcactttc tcaacatcca tgcactgcaa
    13081 cgcctttgtc gggcaggctt caacacaggc cggtcccgtc gcccgatgcc agcacagatc
    13141 gcacttaatc gccctcgcct ttttgcgcga agaaacgacc tccatcgtac caaacgggca
    13201 agccagcata cagcttttac acccaatgca acgtgattgt tcaacgaaaa tatgcccatg
    13261 ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt cacactgatg
    13321 gcaggctaca gccgtggtcc atgtgtggtc tttaatgaca cgaatacggg aaataaactc
    13381 gtctggtgac aacgcagcgc aatcctgatt ttcctgatgc gacactgcgc aagccacttc
    13441 acaggtacgg caaccgatac atttcgtcgc atctgcaata ataaaccggt tcatgatcac
    13501 tcctttgcac taaaacagca gagtgccaga gagcgcgtct ctactgcttt gatccaacga
    13561 aggaaatgtt aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc ctggtcgtcc
    13621 ctgacagtaa atcccagttc attagaaata gcctgcgcgg tttcacgcag tggtttcagg
    13681 agatttttct cccccacctg tttcagacgc gatgtcgaaa gagaaatcga cacggcgtac
    13741 ggcacccgcc catgaatatc aaacaccgga acagcaatac aagagacgcc gagttcgttt
    13801 tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg
    13861 ggcagctcgg taatggtatt gcgggttaac ggctggatct catgctgatg gctttcccag
    13921 tatgacttca cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat tgcggaacag
    13981 tagagcggca tatgctggcc tatataggca cgggttcgca gcatcccggt tgtgggttcc
    14041 agcttataaa tcaaaatagc gtgatcgtct tcgcggctcg agaagttaat ggtttcacca
    14101 gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat gaatgatatt cagcgaagac
    14161 agcgccttct gcccgacggc aataaatttg gtagtcaggc ggtaactccc tgcggcgggc
    14221 gcggtggtca catagccgca ggattgtaat ccctgcaata agcgatggac ggtactctta
    14281 tttaaaccag ccagctccga aagatgcgcc aacggacaac cgtttggata gttgctcaaa
    14341 atctcaatca gcatcaaccc gcgaaacaga ctctggcttc cggctggacg ctctttttcc
    14401 tgcgccatct cgttctcttt ttttcccatc acttctttcc ccaatttgtc gcgtcctgat
    14461 ggtagcgcaa agtgtgtcgt agttcacgat ctcgacagat aatttataac cacttgattt
    14521 ttaagttttt tgaaattcat caatcagatt gctttgttaa aaagcgatcg atatatttga
    14581 aatcagattt cgcatattga aattttatgc caaaaaagcg atcaaaaaaa acaaggaagc
    14641 caaggatgaa agtgacattt gagcagttaa aagcagcctt taatcgagtc ttaatttcac
    14701 gcggcgttga cagcgaaacg gctgatgcct gtgccgagat gttcgcccgc accaccgaat
    14761 ccggcgttta ttctcacggt gttaaccgct tcccacgctt catccaacaa ctggataatg
    14821 gcgatatcat tcctgatgcc gaagccaaac gtataaccac tcttggcgct atcgagcaat
    14881 gggacgctca gcgttcaatc ggcaatctga cggcgaaaaa gatgatggat cgcgccattg
    14941 aactggctgc cgatcacggt attggtctgg tagcactacg taatgccaac cactggatgc
    15001 gcggcggtag ctacggctgg caggcggcgg aaaaaggcta tattggcatt tgctggacca
    15061 actccatcgc cgtaatgccg ccgtggggcg caaaagagtg tcgcatcggc actaacccgc
    15121 tgatcgtcgc cattccttct acgccgatca ccatggtcga tatgtcgatg tcgatgtttt
    15181 cctacggcat gttagaagtt aaccgcctgg caggtcgtca gctcccggta gatggtggct
    15241 ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaaaaat cgccgcattt
    15301 tgccgatggg ttactggaaa ggttccggta tgtcgatcgt gctggatatg atagccaccc
    15361 tgctttccga cggcgcatcg gttgccgaag tcacccagga taacagcgac gaatatggtg
    15421 tttcgcaaat ctttatcgcc attgaagtgg ataaattgat cgacggtgcc acccgcgatg
    15481 ccaagctgca acgcatcatg gattacatca ccaccgctga acgcgccgat gaaaaccagg
    15541 ccatccgctt acccggccat gaatttacca ccctgctagc cgaaaaccgc cgtaacggta
    15601 ttaccgttga tgacagcgtg tgggcaaaaa tccaggcgtt ataaggagat aagcatgatt
    15661 tttggacata tcgcgcagcc tgacccgtgt cgtttgcctg ccgccattga aaaggcgctc
    15721 gattttctgc gcgctaccga tttcaacgcc ctggagccgg gcgttgtcga aatcgacggc
    15781 aaaaatattt atgcgcaaat tatcgattta acgacgcgcc cggcagaaga aaatcgcccg
    15841 gaagtccatc gtcgctatat tgatattcaa tatctggcgt ggggcgagga gaaaatcggc
    15901 attgctattg atacgggaaa taataaagtc agtgagtcgt tattagagca acgagatatt
    15961 attttttatc accattgcga aaacgaatca tttattaaaa tgacacccgg tagttacgcc
    16021 attttcttcc ctcaggatgt tcatcgccca ggttgtattc tgcaaacagc ctccgagata
    16081 cgaaaaattg tggttaaagt cgcattaacg gcgctgaatt aaacggataa gcgtccatga
    16141 cagaacgcct gggtaatgtc attactctaa ccatattaat aattcaggtt gctgcacatg
    16201 caacttgaat tatgacaaac atagcctcaa ggaatagcta tgaaaaaaat acttgaagca
    16261 atactggcga ttaatctcgc cgtactttcc tgtattgtgt ttataaacat tattttaaga
    16321 tatggttttc aaacaagtat tttatctgta gatgaattgt cgcgttattt atttgtctgg
    16381 ttaacattta ttggcgcgat tgtggctttt atggataacg cccacgttca ggtcaccttt
    16441 ctggtggaga aactctcccc cgcatggcag cgacgagtcg ctctggttac ccattcctta
    16501 atcttattta tttgtggcgc actggcctgg ggcgcaacgc taaaaacaat tcaggactgg
    16561 agcgattatt caccgatcct cggtttgcct atcggcctga tgtatgccgc ttgtctgccc
    16621 accagcctcg ttatcgcatt ctttgaactg cgtcatttat atcaactcat cacgcgtagc
    16681 aattctctaa cgtcgccacc gcaaggagct taatcatggc tgtgctgatt tttctgggct
    16741 gtctgttggg tggtattgct atcggtttgc ctattgcctg ggcactgttg ttgtgcgggg
    16801 cggcgttaat gttctggctg gagatgtttg atgtccagat catggcacaa acgctggtga
    16861 acggagccga cagcttctcc ctgctggcga ttccgttctt tgtgctggcg ggtgaaatca
    16921 tgaatgcggg cggcttgtca aaacgcattg ttgacctgcc gatgaaactg gtggggcata
    16981 aaccaggcgg gctgggctac gtcggcgtgc tggcggctat gattatggca agcctttctg
    17041 gttctgccgt tgcggatacc gccgccgttg ccgctctact ggtgccgatg atgcgcagcg
    17101 ccaactaccc ggtcaaccgg gcggcggggc tgattgcctc tggcggcatt atcgcgccaa
    17161 ttattccccc ttccattccg tttattattt tcggcgtttc cagcggatta tccatcagta
    17221 agctgtttat ggcgggcatt gcccctggca tgatgatggg cgcaacgctg atgcttacct
    17281 ggtggtggca ggccagtcgt cttaacttac cccgccagca aaaagcgacg atgcaggaaa
    17341 tctggcactc ttttgtctcc ggtatctggg cgctgtttct tccagtcatc attattggcg
    17401 ggtttcgctc cggtctgttt acccctaccg aagccggggc cgttgctgct ttctatgcgc
    17461 tgtttgtcgc cacagttatt taccgtgaaa tgaccttcgc cacactttgg catgtgctca
    17521 ttggcgcagc gaaaacgacc tcagtggtga tgtttctggt ggcctcggca caagtttccg
    17581 cctggctgat caccattgct gaactgccga tgatggtttc tgatttactg caaccgctgg
    17641 tcgattcacc gcgtctgctg tttatcgtca ttatggtggc tatcctgatt gtcggcatgg
    17701 tcatggattt aacgccgacg gtattaattc ttaccccggt attaatgcct ttagttaaag
    17761 aggcaggaat cgatccgatt tatttcggtg tcatgtttat cattaactgc tcaatcggct
    17821 taattacacc gcctatcggt aatgtgctta acgttatttc aggggtggca aaactcaaat
    17881 tcgatgatgc agtcagaggc gttttccctt acgtcctggt tttatattca ttattagtcg
    17941 tatttgtttt tattcccgat ctcatcatcc tccctttgaa atggattaat taaaaggaaa
    18001 atattatgaa attacgctct gtaacctacg cattattaat tgccggagtg gcggcgttca
    18061 gcacatcttc tctggcggca caatctttac gtttcggtta tgaaacatca caaaccgact
    18121 cgcaacatat tgcggcgaaa aaattcaatg aattattaca ggaaaaaacc aaaggcgaac
    18181 tgaaattaaa attgtttccg gatagcaccc tcggtaatgc gcaggcgatg atcagcggcg
    18241 tgcgtggcgg caccatcgat atggaaatgt ccggctcgaa taactttgcc gggttatcac
    18301 cagtgatgaa cttgcttgat gtccctttcc tgttccgcga taccgcccac gcgcataaaa
    18361 cgctcgacgg caaagtcggt gatgacctga aagtctccct tgaaggtaaa ggtctgaaag
    18421 tgctcgctta ctgggaaaac ggctggcgcg atgtcaccaa ctcgcgcgca ccggttaaaa
    18481 cccccgccga cctgaaaggg ctgaaaattc gcaccaacaa tagcccgatg aatatcgccg
    18541 cattcaaagt ctttggcgct aacccgattc cgatgccgtt tgccgaagtc tataccgggc
    18601 tggaaacccg cactatcgac gctcaggaac accctatcaa cgtcgtctgg tcagcaaaat
    18661 ttttcgaagt acagaagtac ctttctctga cgcaccacgc ctattcgcca cttctggtgg
    18721 tgatcaacaa agcgaagttt gatggcttaa gcccggagtt ccagcaggcg ctagtttcat
    18781 ctgcacaaga agcgggtaac tatcagcgca aactggttgc cgaagatcag caaaaaatca
    18841 tcgacggtat gaaagatgcg ggcgtggaag tcatcaccga tctcgaccgc aaagccttta
    18901 gcgacgcgct gggtactcag gtccgcgaca tgtttgtaaa agatgttccg cagggcgctg
    18961 acctgctgaa agccgtggat gaggtgcaat aaaccatgac gcaatactgg ctggggttag
    19021 attgtggcgg tagctggctg aaagccgggc tgtatgaccg cgaaggccgg gaggcaggcg
    19081 tgcagcgcct gccgctgtgc gcattaagcc cgcagccagg ttgggcagag cgcgatatgg
    19141 cagaactgtg gcaatgctgc atggctgtca ttcgcgccct gcttactcat actggcgtta
    19201 gcggggaaca aatagtgggt atcggtatct ccgcacaggg aaaggggttg tttttactgg
    19261 ataaaaacga caagccgctc gggaatgcta ttttgtcctc ggatcgccgg gcgatggaaa
    19321 tcgttcgtcg ctggcaggaa gatggcatcc cggaaaaact ctacccgctg acgcgacaaa
    19381 ccttgtggac cgggcatccg gtgtcgctgt tacgctggct gaaagagcac gaaccggaac
    19441 gctacgcgca aattggctgc gtgatgatga cgcacgacta cctgcgctgg tgtttaaccg
    19501 gcgtcaaagg ctgtgaagag agcaatattt ccgagtccaa tctctacaac atgagtcttg
    19561 gggaatatga cccgtgcctc accgactggc ttgggatcgc tgaaatcaac cacgccctgc
    19621 cgcctgttgt cggatctgcc gaaatctgcg gggagatcac cgctcagaca gccgcactga
    19681 ccggtctgaa agcgggtacg cccgttgtcg gcggcctgtt tgatgtggtt tccaccgcac
    19741 tctgtgccgg gatcgaagac gaatttaccc ttaatgcggt gatgggcacc tgggcggtga
    19801 ccagcggcat aacccgtggt ttacgtgacg gtgaagcgca tccgtatgtc tatggtcgct
    19861 acgttaacga tggtcaattt atcgttcacg aagccagccc tacctcttcc ggcaacctcg
    19921 aatggtttac cgcacagtgg ggagaaatct cgtttgatga gattaaccag gccgttgcca
    19981 gcttgccgaa ggccgggggc gatctctttt tcctgccgtt cctgtacggc agcaacgccg
    20041 gactcgagat gactagcggt ttctacggga tgcaggccat tcacacccgc gcacacctgt
    20101 tgcaggccat ttatgaaggc gtagtgttca gccatatgac ccatctcaac cggatgcgcg
    20161 aacgttttac tgatgtgcac accctgcgcg tcactggcgg cccggcgcac tccgatgtct
    20221 ggatgcaaat gctggcggac gtcagcgggc tgcgtatcga actgccgcag gtggaagaaa
    20281 ccggctgttt tggcgcggcg cttgccgccc gcgtcggcac cggggtttat cacaacttca
    20341 gcgaagccca gcgcgatcaa cagcacccgg tgcgcacact gctgccggat atgaccgccc
    20401 atcagcttta ccaacaaaaa taccaacgct atcagcatct cattgccgca cttcagggct
    20461 ttcacgcccg catcaaggag cacacattat gagccgacca cttctgcaac tggccctcga
    20521 ccactcatca cttgaagacg cacagcgcga cgtgacgctg ttaaaagaca gcgtcgatat
    20581 cgtcgaagcg ggcaccattc tctgtttaaa cgaagggctt ggcgcggtga aagccttgcg
    20641 cgaacagtgc ccggacaaaa tcatcgtcgc tgactggaag gtcgccgatg ccggtgaaac
    20701 gctcgcgcaa caggcatttg gcgcaggcgc taactggatg accatcatct gcgccgcgcc
    20761 gctcgcgacg gtggaaaaag gccacgccat ggcgcaacgc tgcggtggtg aaattcagat
    20821 agaactgttc ggcaactgga cgctggacga cgcccgcgac tggcatcgta ttggcgtgcg
    20881 gcaggccatt tatcatcgcg gtcgtgacgc gcaagccagc gggcaacagt ggtgcgaagc
    20941 cgatctggca cgtatgaagg cgctttccga tatcggcctt gagctttcca ttaccggcgg
    21001 gattacccct gctgacctgc cgctgtttaa agatatccgc gtgaaagcgt ttattgccgg
    21061 gcgggcgctg gcaggcgcag caaatccggc gcaggtggct ggcgatttcc atgcgcaaat
    21121 cgacgctatc tggggaggcg cgcgtgcgta atcatccgtt aggtatttat gaaaaagcgc
    21181 tggcgaaaga tctctcctgg cctgagcggc tggtgctggc gaaaagctgc agttttgatt
    21241 ttgtcgaaat gtcggtggat gaaaccgacg aacgtttgtc acgccttgac tggaacgccg
    21301 cacaacgtgc ttcgctggtc actgcgatga tcgaaacggg tgttgccatt ccgtcgatgt
    21361 gcctgtccgc acatcgtcgc tttccctttg gcagccggga caaggcggtg cgcgaacggg
    21421 cgcgggaaat catgagtaaa gcgattcgcc tggcgcgcga tctcggcatt cgcaccattc
    21481 aactggcggg ttatgacgtc tattacgaag accacgacga aggcacccag caacgttttg
    21541 ctgaagggct ggcgtgggcg gtcgaacagg cggcggcatc acaagtaatg ctggcggtgg
    21601 agattatgga taccgcgttt atgaactcca tcagcaaatg gaaaaagtgg gacgagatgc
    21661 tcgcctcgcc gtggttcacc gtctacccgg acgtcggcaa cctcagcgcc tggggcaatg
    21721 atgttcccgc cgaactgaaa ctgggcattg accgtattgc cgcaatccat ctgaaagaca
    21781 ccctgccagt caccgggcaa aatcccggac agttccgtga tgtgccgttt ggcgaaggct
    21841 gcgtcgattt cgttggcatc ttcaaaacgc tgcatgaact gaactatcgc ggttctttcc
    21901 tgattgagat gtggaccgaa aaagccaaag agccggtgct ggagattatt caggcgcggc
    21961 gctggattga agcgcgtatg caggaggctg gatttatatg ttagagcaac tgaaagccga
    22021 cgtgctggcg gcgaatctgg cgcttcccgc tcaccatctg gtgacgttca cctggggcaa
    22081 tgtcagcgcg gtagacgaaa cgcggcaatg gatggtaatc aaaccttccg gcgtcgagta
    22141 cgacgtgatg accgccgacg atatggtggt ggttgagata gccagcggta agggggtgga
    22201 aggcagcaaa aagccctcgt ccgacacgcc aacgcatctg gcgctctacc gtcgctatgc
    22261 cgaaatcggt ggcattgtgc atacccactc gcgccacgcg accatctggt cacaggccgg
    22321 gctggatctc cccgcctggg gcaccacgca cgccgattac ttctatggag ccatcccctg
    22381 cacgcgcctg atgaccgcag aagagatcaa cggcgagtat gaataccaga ctggcgaagt
    22441 gatcattgaa accctcgaag aacgcggcag gagtccagcg caaatcccgg cggttctggt
    22501 acattctcac ggcccgttcg cctggggaaa aaatgccgcc gatgcagtgc ataacgccgt
    22561 ggtgctggaa gagtgtgcct atatgggcct gttctcgcgt cagcttgcgc cgcagctccc
    22621 tgcgatgcaa aacgaactgt tagataagca ctacctgcgt aagcatgggg ccaatgccta
    22681 ttacgggcag taatccctca cgccggggct tcattgcccc ggcactacga attgatatgt
    22741 tccttgctgt aacgtcgctt ccacgcagct ggcgttaaac cggtgtgttt gcgaaaaatc
    22801 tgtcggaaat agccaacgtc attaaacccg cacaacgcag ccacttcagt gagcgatcgc
    22861 gcttcgctaa ttaacagctt ttcggcttcg cgcactcgct ggcgatgaat tgcttctgtt
    22921 aacgtcagat gaaacacccg ccggtagacg cgcccaagat aatcggcatt gcagtacagc
    22981 tcgcgggcta aaagcgccgt ggaaagcggg cgatgatatt gcgtgcggat cagtttctgc
    23041 gctttccagg caagcgtggt acctgcggca tcggcctgtt catcaagacg cgcgtcacgg
    23101 gtgagttgct gcaaaatcag cagcacaata aactccagcg ccggagtgcg ttgatgcttt
    23161 tcctgttcgc ttaagaactg gcgaaacagg gccaccagcg cctgccgatc ggcaaccttt
    23221 gtttgttgcg gcacgctaag atgagtgagc cagcgcgggt cgctatccgg cgctttaacc
    23281 tcaaaatgca gccagtaaaa tttgagatcg gcgggaaaat cctctacccc aacatgctgt
    23341 cggttcggcc agagtaataa actctctccc gcatcgacca gaaatatccg ctcttcttcc
    23401 tgaatcgcta atcgaccttt ttcgacaaag ataatttccc atgactccag ccgccttgcc
    23461 ggatgcctcc ctacgccacg ggaaataaat aatccaccat tttgcacttt aatcggtaac
    23521 gtcatggata attcgagcat tgatatgtaa ttt
//
LOCUS       DAYPHN010000066        23517 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000066 DAYPHN010000000
VERSION     DAYPHN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.066"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..373
                     /locus_tag="ACG0BW_002465"
     CDS             <1..373
                     /locus_tag="ACG0BW_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7259416.1"
                     /translation="LIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRIS
                     PINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQF
                     KNCFAEQEILSEFPQPVSLVG"
     gene            complement(416..1567)
                     /locus_tag="ACG0BW_002466"
     CDS             complement(416..1567)
                     /locus_tag="ACG0BW_002466"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFK7259417.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1581..3176)
                     /locus_tag="ACG0BW_002467"
     CDS             complement(1581..3176)
                     /locus_tag="ACG0BW_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFK7259418.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFENKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3321..4079)
                     /gene="aroD"
                     /locus_tag="ACG0BW_002468"
     CDS             complement(3321..4079)
                     /gene="aroD"
                     /locus_tag="ACG0BW_002468"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFK7259419.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVDSVMAAAKILRKTMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4110..4976)
                     /gene="ydiB"
                     /locus_tag="ACG0BW_002469"
     CDS             complement(4110..4976)
                     /gene="ydiB"
                     /locus_tag="ACG0BW_002469"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFK7259420.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLSFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4988..6253)
                     /locus_tag="ACG0BW_002470"
     CDS             complement(4988..6253)
                     /locus_tag="ACG0BW_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259421.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIALDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6480..7694)
                     /locus_tag="ACG0BW_002471"
     CDS             complement(6480..7694)
                     /locus_tag="ACG0BW_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259422.1"
                     /translation="MKNPYFPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVLG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(7793..8149)
                     /locus_tag="ACG0BW_002472"
     CDS             complement(7793..8149)
                     /locus_tag="ACG0BW_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFK7259423.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKYAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8285..8392)
                     /gene="rprA"
                     /locus_tag="ACG0BW_002473"
     ncRNA           complement(8285..8392)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACG0BW_002473"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8578..9690)
                     /gene="ydiK"
                     /locus_tag="ACG0BW_002474"
     CDS             complement(8578..9690)
                     /gene="ydiK"
                     /locus_tag="ACG0BW_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFK7259424.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEVLEELGEIEKPNK"
     gene            10079..13135
                     /locus_tag="ACG0BW_002475"
     CDS             10079..13135
                     /locus_tag="ACG0BW_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFK7259425.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQSLPVELAETLGKTNTAIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCSEL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDVRSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13132..13542
                     /gene="menI"
                     /locus_tag="ACG0BW_002476"
     CDS             13132..13542
                     /gene="menI"
                     /locus_tag="ACG0BW_002476"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFK7259426.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13642..13830
                     /locus_tag="ACG0BW_002477"
     CDS             13642..13830
                     /locus_tag="ACG0BW_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFK7259427.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14379..14747
                     /gene="sufA"
                     /locus_tag="ACG0BW_002478"
     CDS             14379..14747
                     /gene="sufA"
                     /locus_tag="ACG0BW_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFK7259428.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14756..16243
                     /gene="sufB"
                     /locus_tag="ACG0BW_002479"
     CDS             14756..16243
                     /gene="sufB"
                     /locus_tag="ACG0BW_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFK7259429.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAQKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16253..16999
                     /gene="sufC"
                     /locus_tag="ACG0BW_002480"
     CDS             16253..16999
                     /gene="sufC"
                     /locus_tag="ACG0BW_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFK7259430.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGAVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16974..18245
                     /gene="sufD"
                     /locus_tag="ACG0BW_002481"
     CDS             16974..18245
                     /gene="sufD"
                     /locus_tag="ACG0BW_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFK7259431.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAHSVTHIAVKRGQRPVKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSYLLGGAVLRHNTSSQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18242..19462
                     /gene="sufS"
                     /locus_tag="ACG0BW_002482"
     CDS             18242..19462
                     /gene="sufS"
                     /locus_tag="ACG0BW_002482"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFK7259432.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGTKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19475..19891
                     /gene="sufE"
                     /locus_tag="ACG0BW_002483"
     CDS             19475..19891
                     /gene="sufE"
                     /locus_tag="ACG0BW_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFK7259433.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSS
                     QNSIQGCQSQVWIVMRQNAQGIIKLQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20040..21044
                     /gene="ldtE"
                     /locus_tag="ACG0BW_002484"
     CDS             20040..21044
                     /gene="ldtE"
                     /locus_tag="ACG0BW_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFK7259434.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21108..21344)
                     /gene="lpp"
                     /locus_tag="ACG0BW_002485"
     CDS             complement(21108..21344)
                     /gene="lpp"
                     /locus_tag="ACG0BW_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFK7259435.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21655..23067)
                     /gene="pykF"
                     /locus_tag="ACG0BW_002486"
     CDS             complement(21655..23067)
                     /gene="pykF"
                     /locus_tag="ACG0BW_002486"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFK7259436.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23075..23269)
                     /locus_tag="ACG0BW_002487"
     CDS             complement(23075..23269)
                     /locus_tag="ACG0BW_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259437.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         6100 a         5691 c         5928 g         5798 t
ORIGIN      
        1 tctgataaaa gatattcttt tctatctgaa taataactat cgcgaaaaaa tcactttaga
       61 gcagttatcg aaaaagtttc gtgccagcgt cagttatatt tgccatgaat ttaccaaaga
      121 gtatcgcatt tcccctatta actatgttat tcaacggcgt atgacggaag cgaaatggtc
      181 actcactaat actgaattat cacaggcaga gatctcctgg cgtgtgggtt atgaaaatgt
      241 cgatcacttt gccaaactgt ttttgcgcca tgtcggctgt tcgcccagcg attaccgcag
      301 gcaatttaaa aactgttttg cggaacaaga aatcttatct gaatttcctc aaccggtaag
      361 tcttgttgga taaatctggc ggcgcagttt gttaagaagc tgcgccgcct gcagattatt
      421 tgttctgata atctttcagg atctgccgac ccgctacgta aatcataatt tcgtctgtac
      481 cgccgccgat acgttcacaa cggacatcac gccagaagcg agagacgcgc gcctcatcgg
      541 tatagcccag accgcccatg atttgaatcg catcatcaat aacttccatt gcggtacgtg
      601 cgcagtacag ttttgccagc gccgcgctgg tgcgcagtga ctgatgctga tcggcttgcc
      661 atgccacttt caacaccatg ttgcgcatgt tgtcgatctt aatcgccatc agcgccagtt
      721 tttcctggat catctggtta tgaccaatgg gtttaccaaa agcgatacgc tggttggcat
      781 agcgggcggc atcttcaaag gcacactcgg caaagccggt gctgcgcgcg gcgttgatca
      841 ggcgctccat ctcaaagtta tacatcacat tgaggaaacc cattccttct tcgcccacca
      901 tgtcgctctc ttcaacttcc acgttgtcga gatagacttc gcaggtgctg agcatatgcc
      961 agccgatttt atgcagcggg ttaatcttaa tgccgggctt actggagtcg acccaccaca
     1021 gggtgaaggc ttttttggga tctttcggtt gcggatcgcg cgccagcacc agcatatacg
     1081 ggtactcttt cgcaccggta ataaaggttt tctggccgtt gatgtaaacc ttgccgtttt
     1141 tacgcgtgta agtggtggtg gcgctgttgt tatctgagcc tgcgcctggt tccgtcaatg
     1201 ccagggcata ggcgggatca cccgtttcca gggtgctttc cgccgtttta cgtagctgct
     1261 ctgcagaacc gaaacggcgc atactgtgaa tacattgacc gttggtgatc aaaaatgccg
     1321 gggcaccgca ttttgacact tccatcagcg ccagcatttg ggtgacgtaa tccgcaggga
     1381 taccaccaaa ttcttccggc acgccaagca tggaaatacc gttatccgcc agtgcccgca
     1441 taaattcacg cgggtatgtc ccgttttgat cgcaggtgcg gaaatactct tccggaaagt
     1501 tagtcgtaat cagttcgcga atactggcca gcagcagttc ttgttcttca gttaaagaaa
     1561 aatccatcgt gatactcctg ttaatgagcc gcttcaggca ggacaaaacc catcgccgca
     1621 tcgataaata atctttcgtc catcagtttg agttctggcg aaatcactgg ggtgaaatcc
     1681 attttgtcga gaatatcttt ttgtaaatcg acgccagggg cgatttcgat taaatgcagg
     1741 ccgtcttctt tcagcgtgaa tactgcgcgc tcagtgatat aacgaacatc cagccctcgc
     1801 tcgagagcga tttttccgct gaaagtaatt tccggtagtt cccgaataaa tttcttcacc
     1861 cgtccttcct ggacgatatt taatttgccg tcggtaattt ctgtttttaa actgcccgca
     1921 gttaatgtgc cgcagaaaac gattttcttc gaagtggcac tgatatcaat aaatccaccg
     1981 gtgcccatga ttttgccatt gaatttatgc acgccgacgt taccgtgctg gtcgacttca
     2041 gcaaaactca aataacaaac atccagacca ccaccgtgat aaaaatcaaa ctgggacgtc
     2101 atatccagaa tggcgcgggt attcacgttc gcgccaaagg cgataccctg tgaagtaatt
     2161 ccgccaatcg gacctgtttc taccgtcaga ataaagtcat cagcacaacc ttcttcccgg
     2221 gcgaccaggc caatgccgtc agcaataccg acgccgacat tccccaccgc gcctttacgc
     2281 atttcgaata atgcgcgccg tgcaactaat ttacgttggt ttaggggcag gctaagtttg
     2341 gtgctgtcat caagggtgaa gtcaccagaa ataaagcggt taaccggcgc accgccatac
     2401 agttgggttt gatccggatc gaccaccaca atatccacca gataacccgg aatacggaca
     2461 gatttaggat gcagcgtggc tttcttaacc attttctgca cctgcatcat cacaataccg
     2521 ccgttattgt gcaccgcctg ggcaataacc aatgcgtcga gatacatcac ctcatcttca
     2581 aaagtggcgt agccttcgct gtcgcaggtg gtagcgcgaa tgaaggcaat atctggcgct
     2641 atcgctttgt aatagagata ttctttgttt tcaaactcga ccagtttaat caggtcttct
     2701 ttagtgactt cattcagttt gccgccttgc tggcgtggat cgacaaatgt tccgatgcca
     2761 atatcactaa taataccagg ctggtgggct gcggcggcgc gtaaggtttg tgtaagtaca
     2821 ccttgtggat agttataagc aataatttta ttttgttctg cgagatcaga aatacgcggc
     2881 gattgtcccc agtgaccgca taatgcccat ttcaccagac cttcttgcgc cagcggacta
     2941 ataccacggt cggcgcgatc gccaagccct gtcggactaa taatcgataa attacgtggc
     3001 gtttgagtct gtttatattt atcagcaaga gctgtaatta acgtggtggc ttccagaata
     3061 ccgccgccag cgcctaacac acaaagtgtt gcttcgtcgg gaatataatt caccgcttcc
     3121 tgtgccgaca ggaccggcac tcgtccatta atacgaggtg gttttacagg tttcattgtt
     3181 aattcctgat tcaggtgtga ttttccctac gccagatgga gaattagcgc acagagactc
     3241 acgaaatatt tttattaaat acggttaaat atattttttt agttcggcgg gggaggatgt
     3301 tcccgccgaa atattattgc ttatgcctgg tgtaaaatag ttaataccgt gcgcaaatca
     3361 tttaccgaga tttgccctgg tgcagaggcc ttttttaccg caccaaaagt tgccgccgag
     3421 ccaaatactt caccagccag acgagaaatt acgccagttt ttgccattga catcgtgatg
     3481 attggacgat cggcatactg ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca
     3541 tcgctggtac tttgcggcat cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt
     3601 ttgcgcagac gggcaatgat ttcttcggct tccggcgttt tatggaagtc atggttggac
     3661 atgacaactt tcacatcatg cgcgtgggcg taggcgacgg tttctttaac ctgatcatcg
     3721 ccggtaaata actccagatc gatcatatca accaggccgc tgtcgatggc tgcacgattg
     3781 agtgcaatat aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc actgcggaag
     3841 gtaaacagca gcggtttttc tggcatggtt ttacggagaa tttttgccgc cgccatgaca
     3901 gaatccacat tggagaggtc ggcatagtgg tcaacacgcc attccagaat atcaaagtcc
     3961 gcttcacgat aggcgagagc ttcagatttc acgctggcga tatctttcgc catcagcgag
     4021 acgatgattt taggtgcgcc cgtaccaatg acgagatctt ttacagttac ggttttcatt
     4081 ttttaccctt tctgcacgcg gtcagcctgt caggcaccga accccatgac ctgtttaaca
     4141 tattccagag ggaaatcttt gccagtccat aacgtgaact gttcagcccc ttgccacaac
     4201 aacatgccgt atccatcaat cgttttgcaa ccagcttgtt gcgcctgctg caataacttc
     4261 gtcatatgcg ggttatacac gcattcagtg accagaagtc ccggatgtaa cagactgata
     4321 tcattaacca atgattcatt ctcaaggggt ttcataccca cttttgtgcc attggttaaa
     4381 atgtcggcgg aagccagggc ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg
     4441 acgacacaat cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac
     4501 tcatcccgac ggttaaagag tttaatttct tttaaacctt caattgcccc ctgcgcgcca
     4561 atcgccgttg aggcacctcc ggcccctaac agcaccatcg ttttgccttt gatatcaaaa
     4621 ccgctctctt taatggcgcg aatatggccc gtgccgtcgg tgttatagcc acgcagatag
     4681 ccatcatcat taacgatggt gttgatggca cccaccagtt tggccgctgg cgttaattca
     4741 tcaacatatt cacacgccag ttgtttgttc ggcattgata caccagttcc gcgcattttg
     4801 agggctttta atccttcaat tgctccggga aaactatcgt tatccacttc aaaggccata
     4861 taggtaaatg acaatcccgc tttttctaag gctttattct gcatttcagg cgataaactg
     4921 tggcggatgg gataggccat cagcccaatc aattcgtatt ttgcggtaac atccataatt
     4981 aactccttta acctctatgc ttaattgtgt ttaaccgggt gctaaattta cgctcgccaa
     5041 accgcacatc attttcagga ataatgaaaa cgcggtaata gcggataaag acaataattg
     5101 cggtaatcag ggccagcagc gcgaaagtaa aatcgagagc aatgatatat tgcaggccga
     5161 tgttcgacag ataaccggta atcagcggaa taacaaaatt agccagtcca cccatcatca
     5221 tatatatact ggtgactttg gctttgcttt ttgggaaaaa ctctgacatg accgaaacgc
     5281 cgagctgtaa aatgccgcca gctgctgaga aaccgataac aaaggctccg gcattacaca
     5341 ccaatggaga agggtacagg tagataatgg cagctgttat tgttgccagt gcagagttaa
     5401 atacattagc ccagatgggc cggaccattt ttttcagcag tgcggcaaaa ataaagacac
     5461 agaccaacga gcccatactg taataagaga tggtttttaa tgcctcagct tctgacatac
     5521 cagcaaaagc catcgcatat ttgggcatcc acaccacaat cacataaaag gtcgagaatg
     5581 cggctacacc gaacagtact gatgaaacac cttccagcca gactaacggt ttgctgttca
     5641 tttgcggtaa ttcattagct acgctggcgt ccaccaactg gctggggaat ttgcttttca
     5701 acagcatcag tgtgatcagt acaaacagaa tacccggaat aatcagccca tagccgtacc
     5761 agatgttatt gagcaacata tagctcacca gcattgggta gaacatttgc ccgaatgaca
     5821 ccatcgcttt aaccagtatg accgccgaac cagaggcttt cggaaagcat tccatgagcg
     5881 cggggtagcc acccgtatcc agcgctgaat tagcgatacc tacgcacact gccagaccgt
     5941 aggcgagagt taaattcggg caagcgggaa taccaaagaa gaatagcaga tacattatta
     6001 ctgccattaa tatcaccgcc cgacgaccaa acttatcgga gatcacaccg aagaataaaa
     6061 tactgatcaa tcgccccaaa ccgataccgg aaattaaata ggcaatgccc gcgttgtcag
     6121 tggaaaactt ttctgccaga gatgacatat tttgggcaag cgtaataaca ctaatgccgt
     6181 gcaggaaata gctgaagtaa atacaaagaa cagccaggat aaatggcgtg ctgaaagcct
     6241 tattttgaga cattttcaca gctccttgct gaaagataat ttcgttaccg tacgtattgt
     6301 tctccgacgg tttacataat tactatactg aagatgattt tccctgggta catggcataa
     6361 agtttatgtt tccaggaata ttgcatgtca tagtttgttt ttgatcgcga ttttccttag
     6421 ttagcgcaga aatagttatt tatccgtaag atcttgcatg acctgaaaag aatattgcat
     6481 tacccacccg gagcgacatt ttcctttagc gagtgatgcc gcgtttcttt gcggctgcgt
     6541 aagccgataa acagtgccag taaaaagccg atggcagcga tggcggtatc gaaccacata
     6601 atatcggcaa tacttctttg ggacagatga gccgtaatca acggaatggt aaatgttgca
     6661 atactgcccg cactgtaata gatccctgta gctttacctt tagcataggg gaaacgttca
     6721 gccattaacg tcaggccaat ttgcacaaca cctccagcag aggtaaaacc aatgacaaaa
     6781 gcaaatatta tcaccacata aaatgtggga tgcaggcaga cggtaaacag agcaataaat
     6841 gagataaagg tgtacagcat cagtaatgtt gttgggcgaa cggtattacg aatgagtgga
     6901 gcggtaataa atacacaaag cagcgaaccc acggtgtaga tactaagtag tttgatcgac
     6961 atagtgtatg acatgcctgc aacaaattgt ccgtactgtg ccagccactg gctaaccaga
     7021 taaaacgttg ccattgagat ataaccatat aaggtataac tggctaaatc gataattgaa
     7081 cagcgatgtt ccgtagatct ggtggttttc tttatgacag gtaagcgacg acccggatgg
     7141 ggtgggaacg tacaacggta taaaaacaga gcgttaataa acataatgcc tgcagcaatc
     7201 ataaaggacc aaccgaacca cagttcagcc cacactaaca ggctaatgat tagcggtaac
     7261 aaaaattgtc cgctggaaac aaatgcttta attaaaatat tggctgtccc cggtgagcgt
     7321 ggaaaagctt ccatcaaact gggatatgtg cctgcatcga gaaaactgtt tgccattccc
     7381 gccagaaagc ccaaaacata agcgataata atgttattgg tgtgcaggat gccaaaaaag
     7441 aaggccatat agcagcacat cccgagcatg ataaaagggc ggcgaccaaa gcgatcggat
     7501 aataatcctg caaaaagcaa gacacttaat cgaccaatac ccagcgatga gataactatt
     7561 gagacgcctg cggcattagt ctgccaaagc gtctccagcg aggccatatt caggctcatc
     7621 aaaatgacgc ccataccatg caccaggtaa ttaaaataca accccagtgc ggtagggaaa
     7681 tagggatttt tcataggttc tacttagcaa aggtgcttta aaaagtgaag cgatgttgtg
     7741 tcagcatccg ttcattattg ttgtaacaac gacaattcct ttctttggaa aattaacaaa
     7801 ggcgctccag atcgtgtgca tataattcag cggctaccga ttgatagatc ttccagtcaa
     7861 taaaattgcc atcagggtag acttgttgaa acgcggttaa gtcggggaaa aaacgcatgg
     7921 tgttgttgcc gatgcggtta tttattttct ctatgatggc atgaagatgt ttttttcttt
     7981 gctgacgcat agccaacagt tgctcgacta cacgatcagg aatggcgtat ttgccatttt
     8041 cccattgacg ccagctttca ctgtcaccgg tctgggcaat ccaggtcgcg cattcatcaa
     8101 tagtcatggc aaaaatatgg cggagtgcct ggagttcata agcgttcatc gtgtaatggc
     8161 tgcatgaaat aaacaataaa atataaataa cattatcagc ctgctgacgg cttgaagaga
     8221 gtcacagtat cttgtgcaac attattggaa agcgtgaggg gcgaggtagc gaagcggaaa
     8281 aatgttaaaa aaaagcccat cgtgggagat gggcaaagac tacacacagc aattcgttgt
     8341 ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc gtgagctaat
     8401 agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttaa aaactgtaaa
     8461 gaatttgcga caggtactac cagctgaatg gcggtaagca ggaaaatatg gggaagagca
     8521 agcaaatatc aattagcagg ggttaacaat caggcggcag agccgcctga tgagaaatta
     8581 cttattcggt ttctcgattt cgccaagttc ttcgagaact tcttccggtt ggtcagtcgg
     8641 cggtgggact tcttccaccc acgcggcaaa aagacgccag gaaacggcta acagaaccgg
     8701 accaataaac aggccgatca tcccgaaagc aatcaaacca ccaataacgc cagagagaat
     8761 caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga cgttatccag
     8821 cgtgccaacc acaccgctcc acactaacaa taccgttccc caggtggtat cgccagtcca
     8881 gtagagccag ataatcgccg gaatcagtac cggcaacggt ccaagctgga caaggcagga
     8941 gaggatcatt aacaccgtga gcaaagttgc ataaggtacg ccggatacgg cgaggccgat
     9001 accgccaagc actgcctgta ctaacgctgt caccacaaca cccagcgcca ccgcgcggat
     9061 agcttgtgcc gccagcagca cggcggcatc tccacgaacg cctgccagac gggttgcaaa
     9121 atggcgaatg ccttgcgcca cttgttcacc gcgccagtac agcagggcgc tgaagagaag
     9181 catcagcgca caatgcacca taaagcgccc gatatgcgcc gcctgcccaa cgaaccaggt
     9241 ggtggtggtg ccaatataag gacggacttt tgccatgatc gccgtccccc ccatatccag
     9301 caagttgtgc cagcctgcat acagcttcgc gccaatcacc ggaatggtat taagccacgc
     9361 cagatcgggt aacgtcatgt caccgctgga aatggcttta attagcgggc cgctgccgtc
     9421 gacgatactg ttaaccagca aggcaatagg gatgataaac accatcacta ataacagcgt
     9481 catcaccaga acggcgagag accggcgacc aaacatgatc ttttgcaaac gtaacaatac
     9541 cggccaggtg gcgataacca ccgtaccagc ccatgcaaag ccgagaataa agggttgtac
     9601 aatccacaga catgccacaa tcatgatggc taaaaagagc accgaaagca gaatttgtgc
     9661 gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt gtgcgtcaaa
     9721 tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa tttcagcggt
     9781 ttttaacagg ccgattctgc ccttaattct gatgggcgtg ttagaaaaaa atgtggtaca
     9841 acaactcagg caacacgcaa acgatttact cgtcgtattt caagttgcat gtgctgcggc
     9901 tacgttcgtt caccccagtc acttactatt gtaagctcct ggagttcact cgcttgccgc
     9961 cttcctgcaa ctcgaaatcc atagagtaaa taaacatcac ctttcagtgc gctattgcag
    10021 ccgcctgaaa gaatagtgta tcgacctaca tcacagaccg caggaaaggg tcaatataat
    10081 gattccacag atttcccagg cacccggcgt cgttcaactg gtgcttaatt ttttgcaaga
    10141 gctggagcaa caggggttta ccggcgatac ggcgacaagt tatgccgatc gtctgacaat
    10201 gtcgaccgac aacagtattt accaacttct ccccgatgcg gtggtatttc cgcgttcaac
    10261 cgcagatgtg gcgctgatcg cccgtcttgc cgcgcaggaa cgctattcat cgctgatctt
    10321 taccccccgc ggcggcggca ccggcactaa cggtcaggcg ctcaaccagg ggattattgt
    10381 tgatatgtcc cgccatatga accgcatcat cgaaattaac cctgaagagg gctgggtgcg
    10441 cgttgaggcc ggggtgataa aagaccaact caatcagtac ctgaaaccgt tcggctactt
    10501 ttttgcgccg gagctttcga ccagcaaccg ggcaacgctc ggcgggatga tcaataccga
    10561 tgcatccggt cagggatcgc tggtctatgg caaaacgtca gatcacgtac ttggcgtacg
    10621 cgcggtgttg ttggggggcg atattctcga tacgcaatct ttacccgtcg aactggcaga
    10681 aacgctgggt aaaaccaata ccgcaatcgg gcgaatttat aacacggttt atcaacgttg
    10741 ccgtcagcaa cgccagttaa tcatcgacaa cttccccaaa cttaaccgct ttcttaccgg
    10801 ttacgatctg cgtcatgtct ttaacgatga gatgaccgag ttcgacctga cgcgcattct
    10861 gacgggttca gaagggacgc tggcctttat taccgaagcg cggctggata ttacgccctt
    10921 gcctaaagtg cgccgtctgg tgaacgtcaa atatgactct tttgactccg cgctgcgtaa
    10981 cgcgccgttt atggttgagg cgcgggcgct ttcggtagag acggtggact caaaagtgct
    11041 gaatctggcg cgggaagata ttgtctggca ttccgtcagc gagttgatta ccgatgtgcc
    11101 tgacaaagaa atgctcgggc tgaacattgt ggaatttgct ggtgatgatg agacgctgat
    11161 tgatgagcga gtaaatgcac tctgtgcgcg gctggatgaa cttatcgcca gtcagcaagc
    11221 aggtgtgatc ggctggcagg tatgcagtga gctggcaggt gttgagcgta tttatgcgat
    11281 gcgcaaaaaa gctgtcggcc tgctcggtaa tgccaaagga gcagccaagc caattccgtt
    11341 tgctgaggat acctgcgtac cgccggaaca tctggcagat tatattgctg aatttcgcgc
    11401 gctgctcgac agccacggct taagctacgg tatgttcggt cacgtcgacg caggtgtctt
    11461 gcacgtccgt ccggcactgg atatgtgcga tccacaacaa gagattttga tgaagcaaat
    11521 ctctgatgac gtggtggcgc tgactgcgaa atacggtggc ttgttgtggg gcgagcacgg
    11581 caaaggtttt cgcgctgaat acagcccggc gtttttcggt gaggaacttt ttgcagaact
    11641 gcgcaaagtg aaagcggcat ttgacccgca taaccgactc aaccctggga agatttgccc
    11701 gccagaaggt ctcgatgcgc cgatgatgaa agtggacgcg gtgaagcgcg gtacattcga
    11761 tcggcagatc cccattgcgg tacgccagca gtggcgcggt gcgatggagt gtaacggcaa
    11821 cggtttatgc ttcaactttg atgtccgtag tccgatgtgt ccgtcgatga agatcaccca
    11881 gaaccggatt cattcaccga aagggcgcgc aacgctggtg cgtgaatggc tgcgcttgtt
    11941 ggcggatcgc ggcgttgacc cactcaaact ggaacaagaa ctgcctgaat ccggcgtcag
    12001 tttgcggacg ttaattgccc gcacgcgcaa tagctggcat gcgaataaag gcgaatatga
    12061 cttctcccac gaagtcaaag aggcgatgtc gggctgtctg gcctgtaaag cttgttcgac
    12121 ccagtgcccc atcaaaattg atgtgccgga gtttcgctct cgttttctgc agctctatca
    12181 cacccgttat ttacgccctc tgcgcgacca ccttgtcgct acggtcgaga gctacgcacc
    12241 gctgatggca cgcgcgccga aaacctttaa cttcttcatt aaccagccgc tggtgcgcaa
    12301 actctcggaa aaacatattg gcatggttga tttgccgctg ctgtcggtcc cctcgctgca
    12361 acaacaaatg gtggggcatc gctcggcgaa tatgacgctg gaacagcttg aagctctcaa
    12421 tgcagagcag aaagcgcgca cggtgttggt ggtgcaggac ccctttacca gctattacga
    12481 tgcgcaagtg gtggcggatt ttgtccgtct ggtcgaaaaa ttaggtttcc agcctgtgtt
    12541 actgccattt tcgccaaatg gcaaagccca gcatatcaaa ggctttctta atcgttttgc
    12601 gaagacggcg aaaaagacgg cggatttcct caaccgtatg gcgaagctgg ggatgccaat
    12661 ggtgggcgtc gatccggcgc tggtactttg ttatcgcgat gaatataaac tggcgctggg
    12721 cgaggaacgt ggcgagttta acgtcttact ggcgaatgaa tggctggcaa gcgcacttga
    12781 ctcacagcca gtggctacag tcagcggtga atcatggtat ttctttggtc actgtaccga
    12841 agttaccgct ttgccgggcg cgccagcaca atgggccgcg atatttgccc gttttggcgc
    12901 gaaactggaa aatgtcagcg tgggttgctg cggcatggca gggacttacg gacatgaagc
    12961 gaaaaaccat aaaaattcgc tcgggatcta tgagttatcc tggcatcagg cgatgcagcg
    13021 actgccgcgt aatcgctgtc tggcgaccgg atattcctgc cgtagccagg taaaacgggt
    13081 cgaaggcacg ggggtacgcc atcctgtgca ggctttactg gagattatta aatgatatgg
    13141 aaacgaaaaa tcaccctgga agcactgaat gccatgggtg aaggaaacat ggtggggttg
    13201 ctggatattc gctttgaaca tattggtgat gacacccttg aagcgacaat gccagtagac
    13261 tcgcggacaa agcagccttt cgggttgctg catggagggg catccgtggt actggccgaa
    13321 agtatcggtt ccgttgccgg ttatttatgt accgaaggtg agcaaaaggt ggttggtctg
    13381 gaaatcaatg ctaaccacgt ccgctcggca cgagaagggc gggtgcgcgg cgtatgcaaa
    13441 ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg aaatcttcga tgagaaaggg
    13501 cgtttgtgct gttcgtcacg attaacgacc gccattttgt gacgttgtca ccagaaaagt
    13561 gtgacgcata ttgcttttgg ttattatctg cacagtaaac ggtggtatac tgtgcaggtc
    13621 ttgtctaaaa gcgaggatgc tatgtctact caacttgatc ccacccagct ggccattgaa
    13681 tttttacgcc gtgaccagag caatctttca cctgcccaat atctcaaacg tctgaaacaa
    13741 ctagaacttg aatttgctga cctgcttact ctctcttctg ctgaattaaa agaagaaatc
    13801 tactttgcct ggcgtctggg cgtgcattga tcctgactta atgctgcaat ttcgccaaat
    13861 cgacctggat taacctccgt ccaggttcga taaccttctt aatactgctt atctaaccgc
    13921 ctgatattgt tcaaaaataa tactaatcgc agtttgtgtt aaaacgacgg cttagcttta
    13981 tgagattatt cttatcgccc cttcaagagc taagccactg agagtgccgg agataagcgc
    14041 cggatggggt agaaaccctt aagcctgtgt cgcacagact taagggtttt cttatttctg
    14101 aacgctgcac ttttttacct atctcttaaa tagtcatttt caatacaact ttttcttttt
    14161 catcacagcc ttaatgctcc ttagataata atcattatct aacaatgaga tacctaattc
    14221 ttagcgtgcc tgttaaccca cacatcaggg tctatgctta ttaaaagcaa gaaaaaagca
    14281 tgttttacgg taaagcccct gcgtttgctg ggttgaactg ataatcatta tcactaacat
    14341 gctgttatac gctgaaagcg atgaagtgag gtaaatcgat ggacatgcat tcaggaacct
    14401 ttaatccaca ggatttcgcc tggcagggct taacgctgac acccgcagcc gctgtccaca
    14461 tacgcgagct ggtggcaaag cagccgggta tggtcggcgt gcgcttaggc gtgaagcaaa
    14521 cgggctgcgc gggctttggc tatgtgctcg acagtgttag cgagccggac aaagacgatc
    14581 tgctgtttga acacgacggt gcgaagctgt ttgtcccgct gcaagcgatg ccgtttattg
    14641 atggcacgga agtcgatttc gttcgtgaag gacttaatca gatattcaaa tttcacaacc
    14701 ctaaagccca gaatgaatgt ggctgtggcg aaagctttgg ggtataggcg gtactatgtc
    14761 tcgtaatact gaagcaactg acgatgtcaa aacctggacc ggcggcccgc tgaattataa
    14821 agaaggattc ttcacccagt tagccaccga tgagctggca aaggggataa acgaagaggt
    14881 ggtgcgcgca atttcggcga agcgtaatga gccggagtgg atgctggagt ttcgtctcaa
    14941 cgcctatcgc gcatggctgg agatggaaga accgcactgg ttgaaagcgc actacgacaa
    15001 gctgaattat caggattaca gctactactc agcaccatcg tgcggtaatt gtgacgacaa
    15061 ctgcgcgtct gaacccggcg cggtgcagca aactggcgcg aacgcctttt taagtaaaga
    15121 ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg gaaggcaaag aggtggcggt
    15181 ggacgccatt ttcgactctg tttcggttgc caccacttat cgcgaaaaac tggcggagca
    15241 gggaattatt ttctgttcct ttggcgaggc gatccacgat cacccggaac tggtgcgtaa
    15301 atatctcggc accgtggtgc cggggaatga caacttcttt gccgcactta atgcggcggt
    15361 agcctctgac ggtacgttta tttatgtgcc taaaggcgtg cgctgcccga tggaactttc
    15421 cacctatttt cgcattaacg cgcaaaaaac cgggcagttt gagcgcacca ttctggtggc
    15481 cgacgaagac agctacgtca gctacattga aggctgttcc gctccggtgc gtgacagcta
    15541 tcagttacac gcggcggtgg tggaagtcat catccataaa aacgccgagg tgaaatattc
    15601 cacggtacaa aactggttcc ctggcgataa caacaccggc ggtattctca acttcgtcac
    15661 caagcgtgct ttgtgcgaag gcgaaaacag caaaatgtca tggacgcaat cagaaaccgg
    15721 gtcagcgatt acgtggaaat atcccagctg tattttgcgc ggcgataact ccattggtga
    15781 gttttactca gtggcgctga ccagcggtca tcagcaagcg gataccggca ccaagatgat
    15841 ccacatcggt aaaaacacta aatcgaccat tatctcgaaa gggatctctg ccggacatag
    15901 tcagaacagt tatcgcggct tagtgaaaat catgccgacg gcaaccaatg cgcgcaattt
    15961 cactcagtgc gactcaatgc tgattggcgc taattgtggg gcgcatacct tcccgtatgt
    16021 cgaatgtcgc aataacagcg cacaactgga gcacgaggca acaacatcac gtattggtga
    16081 agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa gaagatgcca tctcgatgat
    16141 tgttaacggt ttctgcaaag acgtgttctc agaactgccg ctggaatttg ccgttgaagc
    16201 acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga taaggaataa acatgttaag
    16261 tattaaagat ttacacgtca gcgtggaaga taaagctatc ctgcgtggat taagcctcga
    16321 cgttcgtccc ggcgaagttc acgccattat ggggccaaac ggttcgggca aaagtacctt
    16381 atcggcaacg cttgccgggc gagaagatta tgaagtgacg ggcggcgcgg ttgagttcaa
    16441 aggcaaagat ttgcttgcgc tgtcgccgga agatcgtgca ggtgaaggca tctttatggc
    16501 cttccagtat ccggtggaaa ttcccggcgt cagtaaccag tttttcctgc aaacggcact
    16561 taatgcggtg cgcagctatc gcggccagga aacgctcgac cgctttgatt ttcaggattt
    16621 gatggaagag aaaatcgctc tcctgaagat gccggaagat ttattaaccc gttcggtaaa
    16681 cgttggtttt tccggcggcg agaaaaagcg caacgatatt ttgcaaatgg cggtgctgga
    16741 accggagtta tgtattcttg atgagtcgga ctccgggctg gatattgacg cattaaaagt
    16801 ggttgccgat ggcgtgaact cgctgcgtga cggcaagcgt tcattcatca ttgttacgca
    16861 ctaccaacgc attctcgact acatcaagcc tgattacgtc catgtgctgt atcagggacg
    16921 aattgtgaaa tccggcgatt tcacgttggt caaacaactg gaggagcagg gttatggctg
    16981 gcttaccgaa cagcagtaac gcgctgcaac agtggcatca cttgtttgaa gccgagggtg
    17041 caaaacgctc cccgcaagca cagcagcatt tacaacaatt gctgcgtacc ggactgccga
    17101 cacggaaaca tgaaaactgg aaatatacgc cgctggaagg gctgaccaat agccagtttg
    17161 tctgcattgc gggagagata tccccacagc agcgagatgc cttagcgtta acgttagacg
    17221 ctgtgcggct ggtatttgtc gatggacgtt acgtgtcagc actgagcgat gcaactgaag
    17281 gcagcggata tgaagtgagc attaacgatg accgccaggg gttacccgac gctattcagg
    17341 cggaagtgtt tctgcatttg acggaaagcc tggcgcatag cgtgacgcat atcgccgtga
    17401 agcgcggtca acggccggta aagccattgc tgttaatgca tataacccag ggcgtggcag
    17461 gtgaagaggt gaacactgcc cattaccgac atcatctgga tctggcggaa ggtgccgaag
    17521 caacggtgat cgaacatttt gtcagcctta atgatgctcg tcattttact ggggcacggt
    17581 tcactatcaa tgtcgcagcg aacgcccact tgcagcatat caagctggcg tttgaaaacc
    17641 cgctcagtca ccactttgcc cataacgatt tgttgctggc agacgatgcc accgcattta
    17701 gccacagtta cctgctgggt ggcgcagtgt tacgacacaa caccagttcg caactcaatg
    17761 gcgaaaacag cacgctgcgg atcaatagcc tggcgatgcc ggtgaaaaac gaggtgtgtg
    17821 atacccgtac ctggctggaa cacaataaag gtttttgtaa cagccgacag ttgcacaaaa
    17881 ctatcgtcag cgacaaaggc cgcgcggtat ttaacggttt gatcaacgtc gcgcagcacg
    17941 ccatcaaaac ggatggtcag atgaccaaca ataatctgct gatgggcaaa ctggcggaag
    18001 tggatacgaa accacagctg gaaatctatg cagatgatgt gaaatgcagc cacggcgcga
    18061 cggtggggcg tattgatgac gaacagatgt tttatctgcg ctcgcgcggg atcaatcagc
    18121 aagatgccca gcagatgatc atctacgcct ttgctgctga actgacggaa gcactgcgtg
    18181 atgaggggct taaacagcag gtgctggccc gaatcggtca acgactgcca ggaggtgcaa
    18241 gatgactttt tccgtcgaca aagtgcgggc cgactttccg gtgctttcgc gtgaggtaaa
    18301 cggtttgccg ctggcttatc tcgacagcgc cgccagtgca cagaaaccga gccaggtgat
    18361 tgacgccgag gccgagtttt atcgtcatgg ctacgcggcg gtgcatcgcg gtattcatac
    18421 cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc aagcgggcat cgctgtttat
    18481 taatgcccgt tcggcggaag agctggtgtt cgtccgcggc acgacggaag ggatcaatct
    18541 ggtcgccaat agctggggta acagcaatgt gcgggcgggc gataacatca tcatcagtca
    18601 gatggagcac cacgctaaca ttgttccctg gcagatgctt tgcgcacgcg ttggcgcaga
    18661 gctgcgtgtg atcccgctca atcccgacgg tacgttgcaa ctggagacgc tacctacgct
    18721 gtttgatgag aaaactcgcc tgctggcaat tactcatgtc tccaacgtgc tgggaacgga
    18781 aaatccgctg gcggaaatga tcacgcttgc gcatcagcat ggcacaaaag tactggtgga
    18841 tggcgctcag gcggtgatgc atcatccggt ggacgttcag gcgctggatt gcgattttta
    18901 cgtgttctcc gggcataaac tgtatggccc cactgggatt ggcattcttt atgtcaaaga
    18961 agccttgttg caggagatgc cgccgtggga agggggcggt tctatgatcg ccaccgtcag
    19021 cctgagtgaa ggcactacct ggaccaaagc accttggcgg tttgaagccg gtacacccaa
    19081 taccgggggc atcattggtc ttggcgcggc gctggagtat gtgtcggcgc tggggcttaa
    19141 taatatagcc gagtatgaac agaatctgat gcactacgcg ctatcacagc tggaatctgt
    19201 accggatctc actctctatg gtccccagaa cagacttggc gttattgctt ttaatcttgg
    19261 taaacaccac gcctatgatg ttggcagttt tctcgataat tacggcattg ctgtgcgtac
    19321 cggacatcac tgcgctatgc cattaatggc ctattacaac gttcctgcca tgtgtcgggc
    19381 gtcgctggcc atgtataaca cccatgaaga agtggatcgt ctggtgaccg gcctgcaacg
    19441 tattcaccgt ctgctgggat aacagggagg cactatggct ttattgccgg ataaagaaaa
    19501 attgctgcgt aattttttac gctgcgccaa ctgggaagag aaatatctct acattattga
    19561 gctgggccag cgtctgccag aattacgcga cgaagacaga agctcacaaa atagcattca
    19621 gggctgccag agtcaggtgt ggattgtcat gcgccagaat gcgcagggaa ttattaaatt
    19681 gcagggcgac agcgatgcgg cgattgtgaa agggcttatt gcggtcgtct ttattctcta
    19741 cgatcagatg acgccgcagg atattgtcaa tttcgatgtg cgtccgtggt ttgaaaaaat
    19801 ggcgctcact caacatctca ccccatctcg ttcacaaggt ctggaagcga tgattcgcgc
    19861 aattcgcgcc aaagccgctg cacttagcta aactaaagga acagctttca tccggttggc
    19921 tcacattgcc ggatggtcaa tgtttacact gacctgcaca ggagtgtttt ggtttcaggt
    19981 gaacataagc gccatccgac gtttcagcgt gagtctccgg caaatacagg aggtttacaa
    20041 tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc ttttagcgcc atccaggctg
    20101 cctgggcggt tgattatccg ctacctccaa ccggaagccg actggttggg caaaatcaaa
    20161 cgtatacggt gcaggaaggg gataaaaacc ttcaggccat cgcccgacgt tttgatactg
    20221 cggcaatgtt gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa cctggtacga
    20281 cgataactat tccttcgcaa ctgttattac ctgatgcgcc gcgtcagggg attatcgtta
    20341 accttgcaga gctgcgcctt tattattatc cgccgggaga aaatatcgtg caggtctatc
    20401 cgataggtat tggtttgcag gggctggaaa cgccagtgat ggaaacgcgt gtggggcaga
    20461 aaatccctaa cccaacctgg acgcctacgg cgggcattcg tcagcgttct ttggagcgtg
    20521 gcattaaatt accgccagtc gttcctgccg ggccaaataa cccgctagga cgttacgcac
    20581 tgcgcctcgc gcatggtaat ggcgaatacc tcattcatgg caccagtgcg ccggacagcg
    20641 tcggtttgcg cgtcagttca gggtgtattc gcatgaatgc accggatatt aaagccttgt
    20701 tctccagcgt gcggacggga acgccggtga aagtgatcaa cgaaccggtg aaatattccg
    20761 tggaaccgaa cgggatgcgt tatgttgaag tacatcgacc actatcggca gaagaacagc
    20821 agaacgttca gacaatgcca tacacactgc cagcaggctt tacgcaattt aaagacaata
    20881 agggtgtaga tcagaaatta gtcgataaag cgttgtatcg tcgggcaggg tatccggttt
    20941 cggtgagcag tggagcaact cccgcagcca gcaatgcgcc ttcagtagag tcagcgcaga
    21001 atggtgaacc agagcaaggg aatatgttac gcgcgacgca gtagcggtaa atggcaggca
    21061 aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca ggtactatta cttgcggtat
    21121 ttagtagcca tgttgtccag acgctggtta gcacgagctg cgtcatcttt agcagcctga
    21181 acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt caactttagc gttcagagtc
    21241 tgaacgtcag aagacagctg atcgatttta gcgttgctgg agcaacctgc cagcagagta
    21301 gaacccagga ttaccgcgcc cagtaccagt ttagtagctt tcattattaa taccctctag
    21361 attgagttaa tctccatgta gcgttacaag tattacacaa agttttttat gttgagaata
    21421 tttttttgat gggaaggcac ttatttttga tcgttcgctc aaagaagcat cgaacgctgg
    21481 gttttagata aaaaaattga gagaaaacag gatgcttcca tcggattcat cttagataaa
    21541 gtgagattat atagtgaaat ccgatttgat tttttattgc ttctggttat cgattaaata
    21601 aaaaaagcgc ccatcagggc gcttcgatat acaaattaat tcacaaaagc aatattacag
    21661 gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc agtgcaccag aaaccataac
    21721 tacgacgtca cctttgtgtg ccagaccgct ctgcagagcc agttctttac ccagacggta
    21781 gaaatcatca gtagaagtga tctctttaac cagctgcggc acaacgcctt tgctcagtac
    21841 caactgatga gccgtttttt cgttggtggt cagtgccagg atggtggcat ccgggaagta
    21901 tttacgtact gcgcgagcag atttaccgcc ctgggtagca accacgatca gcggggcatc
    21961 cagtttttca gcagtttcaa cggcaccacg gcataccgct tcggtaatgc gcagtttacg
    22021 gttgtcattg ttgaactcga gacggctgtt catcacgcgg tcggtacgtt cgcagatggt
    22081 cgccatgata gaaaccgctt ccagcgggta tttacctttt gcggattcac cagacagcat
    22141 cactgcgtca gtaccgtcga ggatggcgtt tgcaacgtca ccggcttctg cgcgagtcgg
    22201 gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca gtgataacga ctttacgtgc
    22261 acggatacat ttttcgatca tcatcttctg ggcgaagata acttcttcta ccgggatttc
    22321 tacacccagg tcgccacgcg caaccatgat gccgtcagag gcttcgagga tttcgtcgaa
    22381 gttgttgagg ccttcctggt tttcgatttt ggagatgatg tggatgtttt cgccgccgtg
    22441 cgctttcagg tgctcacgga tttcgataac gtcagaacgc ttacgaataa aggaagcagc
    22501 gacaaagtct acgccttgtt cgcaaccaaa gatcaggtcc tgtttgtctt tttcagccag
    22561 tgctggcagg gcaatggaaa cgccaggcag gttcacacct ttgttttcac ccaggtcgcc
    22621 gttgttcagc actttacaga taactttgtt accttcaatg gcagtaactt ccataccgat
    22681 cagaccatcg tcaaccagta cggtgttgcc aacagacagg tcagttgtga aaccttcata
    22741 cgttaccgca accatttcgc tgttgccgat aacagattta tcagtggtga aagtaaaggt
    22801 ctgacccgct ttcagagaaa cgtcgttacc gccttccagt ttcatggtgc ggatttccgg
    22861 acctttggta tcaagcagga tagcggcggt tttaccagtt ttgctcatca cgttgcgcag
    22921 attctgaatg cgctgaccgt gttctgcata gtcaccatga gagaagttca gacgcataac
    22981 gttcatgcca gcgtccagca ttttagctaa catctcttca gattcggttt tcggtccgat
    23041 ggtgcaaaca attttggtct ttttcatgac agtcttagtc tttaagttga gaaggatggg
    23101 agaaacttgc tttctgggcg cactgccgga aagcgaatcc ggtattacga aagtggtggt
    23161 gcctcgctct aaggataggt gacatcgaaa aagcgtgcag aggaatgtgt gcttgtgtct
    23221 cagcccaacg tgagtgcctg tttttgagtc gttgagttct acagtccaaa gtgctgaaac
    23281 cattcaagag tcaattggcg cgcattatac gctgaaatta atccaaaagg aaaacaaaaa
    23341 cagcgtttca aaaaatatac aacttgtaag acaataaatt gttatcaaag atttaacgtt
    23401 ccagggaaaa ggttacgcaa aaacttcgct gtttgcgcaa cgtctggtgc cggggttgtc
    23461 actgtgaaga taagtgaaag ttcatccggt gtataaatac atcatcgctc aattgct
//
LOCUS       DAYPHN010000067        23323 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000067 DAYPHN010000000
VERSION     DAYPHN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.067"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1186
                     /gene="gcl"
                     /locus_tag="ACG0BW_002488"
     CDS             <1..1186
                     /gene="gcl"
                     /locus_tag="ACG0BW_002488"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFK7259438.1"
                     /translation="LIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGWGC
                     IPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHI
                     DIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTL
                     LRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQ
                     AGPLGWTIPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAY
                     LGLIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAP
                     AFEQAKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAVDAPTETCFMH
                     YE"
     gene            1199..1975
                     /gene="hyi"
                     /locus_tag="ACG0BW_002489"
     CDS             1199..1975
                     /gene="hyi"
                     /locus_tag="ACG0BW_002489"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFK7259439.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            2075..2953
                     /gene="glxR"
                     /locus_tag="ACG0BW_002490"
     CDS             2075..2953
                     /gene="glxR"
                     /locus_tag="ACG0BW_002490"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFK7259440.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            3123..4577
                     /locus_tag="ACG0BW_002491"
     CDS             3123..4577
                     /locus_tag="ACG0BW_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFK7259441.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            4637..5998
                     /gene="allB"
                     /locus_tag="ACG0BW_002492"
     CDS             4637..5998
                     /gene="allB"
                     /locus_tag="ACG0BW_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFK7259442.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTE
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            6055..7356
                     /locus_tag="ACG0BW_002493"
     CDS             6055..7356
                     /locus_tag="ACG0BW_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFK7259443.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            7378..8523
                     /gene="glxK"
                     /locus_tag="ACG0BW_002494"
     CDS             7378..8523
                     /gene="glxK"
                     /locus_tag="ACG0BW_002494"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFK7259444.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGPRGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(8653..9438)
                     /gene="allE"
                     /locus_tag="ACG0BW_002495"
     CDS             complement(8653..9438)
                     /gene="allE"
                     /locus_tag="ACG0BW_002495"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFK7259445.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(9449..10684)
                     /gene="allC"
                     /locus_tag="ACG0BW_002496"
     CDS             complement(9449..10684)
                     /gene="allC"
                     /locus_tag="ACG0BW_002496"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFK7259446.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(10706..11755)
                     /gene="allD"
                     /locus_tag="ACG0BW_002497"
     CDS             complement(10706..11755)
                     /gene="allD"
                     /locus_tag="ACG0BW_002497"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFK7259447.1"
                     /translation="MKISRETLRQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12072..13739
                     /locus_tag="ACG0BW_002498"
     CDS             12072..13739
                     /locus_tag="ACG0BW_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFK7259448.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRARDKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADAKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARPDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALALQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            13749..15008
                     /locus_tag="ACG0BW_002499"
     CDS             13749..15008
                     /locus_tag="ACG0BW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFK7259449.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMCWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRSGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            15019..15834
                     /locus_tag="ACG0BW_002500"
     CDS             15019..15834
                     /locus_tag="ACG0BW_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFK7259450.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNEQPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDTRSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            15831..16724
                     /locus_tag="ACG0BW_002501"
     CDS             15831..16724
                     /locus_tag="ACG0BW_002501"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFK7259451.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRRATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(16851..17918)
                     /gene="purK"
                     /locus_tag="ACG0BW_002502"
     CDS             complement(16851..17918)
                     /gene="purK"
                     /locus_tag="ACG0BW_002502"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFK7259452.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(17915..18424)
                     /gene="purE"
                     /locus_tag="ACG0BW_002503"
     CDS             complement(17915..18424)
                     /gene="purE"
                     /locus_tag="ACG0BW_002503"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFK7259453.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(18542..19264)
                     /gene="lpxH"
                     /locus_tag="ACG0BW_002504"
     CDS             complement(18542..19264)
                     /gene="lpxH"
                     /locus_tag="ACG0BW_002504"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFK7259454.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(19267..19761)
                     /gene="ppiB"
                     /locus_tag="ACG0BW_002505"
     CDS             complement(19267..19761)
                     /gene="ppiB"
                     /locus_tag="ACG0BW_002505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFK7259455.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            19935..21320
                     /gene="cysS"
                     /locus_tag="ACG0BW_002506"
     CDS             19935..21320
                     /gene="cysS"
                     /locus_tag="ACG0BW_002506"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFK7259456.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGPGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSSVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(21356..21877)
                     /locus_tag="ACG0BW_002507"
     CDS             complement(21356..21877)
                     /locus_tag="ACG0BW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFK7259457.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRYWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYSTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(21985..22197)
                     /gene="ybcJ"
                     /locus_tag="ACG0BW_002508"
     CDS             complement(21985..22197)
                     /gene="ybcJ"
                     /locus_tag="ACG0BW_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFK7259458.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(22199..23065)
                     /gene="folD"
                     /locus_tag="ACG0BW_002509"
     CDS             complement(22199..23065)
                     /gene="folD"
                     /locus_tag="ACG0BW_002509"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFK7259459.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         5397 a         5818 c         6396 g         5712 t
ORIGIN      
        1 gttaattcag gccgaacgtc cggtgattgt tgccgggggc ggggtaatca atgctgacgc
       61 agctgcactg ttacaacagt ttgctgaact gaccagcgtt ccggtgatcc caacgctgat
      121 gggctggggc tgtatcccgg atgaccacga actgatggcc gggatggtgg gtctgcaaac
      181 tgcgcatcgt tacggtaacg caacgctact ggcgtccgac atggtgtttg gtatcggtaa
      241 ccgttttgct aaccgtcata ccggttcggt agagaaatac accgaagggc gcaaaatcgt
      301 tcatatcgat attgagccga cgcaaattgg ccgcgtgctg tgtccggatc tgggcattgt
      361 ctctgatgct aaagcggcgc tgacgctgct ggttgaagtg gcgcaggaga tgcaaaaagc
      421 gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc cagcagcgca aacgcacttt
      481 gctgcgcaaa acccacttcg acaatgtgcc ggtgaaaccg cagcgcgtgt atgaagagat
      541 gaacaaagcc tttggtcgcg atgtttgtta tgtcaccacc attggtttgt cacaaatcgc
      601 tgcggcacaa atgctacatg tctttaaaga ccgccactgg atcaactgtg ggcaggcagg
      661 cccgttgggc tggaccattc cggcggcgct gggcgtgtgt gccgctgatc cagagcgcaa
      721 tgtggtggcg atttccggtg acttcgactt ccagtttctg attgaagaat tagccgttgg
      781 cgcacagttc aacattccgt acatccatgt actggtcaat aacgcctatc tgggtctgat
      841 tcgccagtcg cagcgcgcgt ttgatatgga ttactgcgta cagctcgcgt tcgaaaatat
      901 taactccagc gaagtgaacg gttacggcgt cgaccacgta aaagtagcgg aaggtttagg
      961 ttgtaaagcg attcgcgttt tcaaaccgga agatatagca ccagcctttg aacaggcgaa
     1021 agccttaatg gcgcaatatc gggtaccggt agtcgtggaa gttattctcg agcgtgtgac
     1081 caatatttcg atgggcagcg aactggataa tgtcatggaa tttgaagata tcgccgataa
     1141 cgcagtggat gcaccgactg aaacctgctt catgcactat gaataaggga gataaataat
     1201 gttacgtttc tctgctaatt tatcgatgtt atttggtgaa tatgattttc tcgcccgttt
     1261 tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt atgttccctt atgactacga
     1321 cattgaagaa ttaaaacagg tgctggcgag taataaactc gaacatacgc tgcacaattt
     1381 accggcaggt gactgggcgg cgggcgagcg gggtattgcc tgtattcctg gccgtgaaga
     1441 agagtttcgg gatggcgtag cagcagcgat tcgttatgcc cgtgcgctgg gtaataaaaa
     1501 aattaactgt ctggtcggta aaacgccggc tggtttcagc agtgaacaga ttcacgcaac
     1561 gcttgtagaa aacctgcgtt atgccgcgaa tatgctgatg aaagaagata ttttattact
     1621 gattgaacct attaaccatt ttgatattcc tggtttccat ctcaccggaa ctcggcaggc
     1681 actgaaactg attgatgatg ttggttgctg caatttgaaa atacagtatg acatttatca
     1741 catgcagcgg atggaaggtg aattaaccaa cactatgacg caatgggccg ataaaattgg
     1801 tcacctgcaa attgctgata atccgcatcg cggcgaaccg gggaccggag aaattaatta
     1861 tgattatctc tttaaggtaa tcgaaaattc tgactacaac ggttgggttg ggtgtgaata
     1921 taaaccccaa accaccacgg aagccggttt acgctggatg gatccgtacc gttaaaacgt
     1981 aacgctattc agacaatgct tttttaggcc gctaagttgg caggggatcg tgttgtctga
     2041 attcaggaaa agcgaaattt aaaagaggtt aattatgaaa ctgggattta ttggcttagg
     2101 cattatgggt acaccgatgg ccattaatct ggcgcgagcc ggtcatcaac tgcatgtcac
     2161 aaccattggt ccggttgctg atgaattact gtcactgggt gccgtcagtg ttgaaactgc
     2221 gcgccaggta acggaagcat cggacatcat ttttattatg gtgccggata cgccacaggt
     2281 tgaagaagtt ctgtttggtg aaaatggttg taccaaagcc tcgctgaagg gcaaaaccat
     2341 tgttgatatg agctccattt ccccgattga aactaagcgt ttcgctcgtc aggtgaatga
     2401 actgggcggc gattatctcg atgcgcccgt ctccggcggt gaaatcggcg cgcgtgaagg
     2461 gacgttgtcg attatggttg gcggtgacga agcggtattt gaacgtgtta aaccgctgtt
     2521 tgaactgctc ggtaaaaata tcaccctcgt gggcggtaac ggcgacggtc aaacctgcaa
     2581 agtggcaaat cagattatcg tggcgctcaa tattgaagcg gtttctgaag ccctgctgtt
     2641 tgcttcaaaa gccggtgcag atccggtacg tgtgcgccag gcgctgatgg gcggctttgc
     2701 ttcctcacgt attctggaag ttcacggtga gcgcatgatt aaacgcacct ttaatccagg
     2761 ttttaaaatt gcgctgcacc agaaagatct caacctggca ctgcaaagtg cgaaagcact
     2821 tgcgctgaac ctgccaaaca ctgcgacctg ccaggagtta tttaatacct gtgcggcaaa
     2881 cggtggcagc cagttggatc actctgcgtt agtgcaggcg ctggaattaa tggctaacca
     2941 taaactggcc tgatacccgc aataaaaatg gccgatatca gaaaatgaat cggccagcaa
     3001 tattaaaaaa agaaagcagc caaagatgtt gcttcagtat taaaaataat atttttattt
     3061 tatttgttcc tcatagctag attaaaacag cgttattcga tacgtgaaat taagagggat
     3121 ttatggaaca tcagagaaaa ctattccagc aacgcggcta tagcgaagat ctattgccga
     3181 aaacgcaaag ccagcggacc tggaaaacat ttaactattt taccttatgg atgggttcgg
     3241 ttcataacgt tcccaattat gtgatggtcg gcggcttttt tattctcggc ttgtctacct
     3301 ttagtattat gctggcaatt atcctcagcg cctttttcat tgccgcggta atggtattaa
     3361 acggtgctgc gggcagtaaa tacggtgtgc cttttgccat gatcctgcgt gcttcttacg
     3421 gcgtacgtgg tgcactgttt cccggattat taagaggcgg gattgctgcc attatgtggt
     3481 ttggcctgca atgttacgct ggatcgctgg cctgcttgat tctgattggc aaaatctggc
     3541 cgggattttt aactctcggt ggtgatttta ccctgttagg gctttctcta ccgggcttaa
     3601 tcactttctt actcttctgg ctggtgaacg tcggaatcgg tttcggtggt ggcaaagttt
     3661 taaataaatt cactgccatt cttaacccgt gcatctatat cgttttcggc ggtatggcga
     3721 tttgggcgat ttcgctggtc gggctcggtc caatctttga ctacattccg agcggtattc
     3781 agaaagcaga aaacagtggc ttcctgttcc tggtggtgat taacgcggta gttgcggtct
     3841 gggcggcacc ggcggtgagc gcatccgact ttacgcaaaa cgcccactcg tttcgtgagc
     3901 aggcgctggg gcaaacgctc ggtttagttg tggcctatat tctgtttgcg gtcgccgggg
     3961 tatgtattat tgccggagcc agtattcact acggcgctga cacctggaac gtgctggata
     4021 ttgttcagcg ttgggatagc ctgtttgcct cgttctttgc ggtactggtt attctgatga
     4081 cgactatctc cactaacgcc accggtaata ttattcctgc tggttatcag attgccgcta
     4141 ttgcaccgac gaaactgacc tataaaaacg gcgtactgat tgccagtatt atcagcctgc
     4201 tgatctgccc gtggaaatta atggaaaatc aggacagtat ttatctgttc ctcgatatta
     4261 tcggcggaat gctgggtccg gtaattggtg tcatgatggc gcattatttt gtggtgatgc
     4321 gtgggcaaat taatcttgat gaactgtaca ccgcacctgg cgattataaa tattacgata
     4381 acggttttaa cctcactgcg ttttcagtaa ctctggtggc cgttatttta tctcttggcg
     4441 gtaagtttat tccctttatg gaaccgttat ctcgtgtttc atggtttgtc ggcgtcatcg
     4501 tcgcctttgc ggcctacgcc ttattaaaga aacgtacaac agcagaaaaa acaggagagc
     4561 aaaaaaccat aggttaatta atcccgatat tgaacattga gttaaaaacc aatctgtatt
     4621 ttacaaggag tttgttatgt cttttgattt aatcattaaa aacggcaccg ttattttaga
     4681 aaacgaagct cgcgttgtag atatcgccgt taaaggcgga aaaattgctg ctatcggtca
     4741 ggatctgggc gatgcaaaag aagttatgga tgcgtctggt ctggtggttt cgccaggcat
     4801 ggttgatgcg cacacccata tttctgaacc gggtcgtagc cactgggaag gttatgaaac
     4861 tggtactcgc gcagcggcaa aaggtggtat caccaccatg atcgaaatgc cgctcaacca
     4921 gctgcctgca acggttgacc gcgcttcaat tgaactgaag ttcgatgccg ctaaaggcaa
     4981 gctgactatc gatgcggcac aactcggtgg cctggtgtct tacaacatcg accgtctgca
     5041 tgagctggat gaagtgggcg ttgtcggctt caaatgcttc gttgcgacct gtggcgatcg
     5101 cggtatcgac aacgacttcc gtgacgtcaa tgactggcag ttcttcaaag gtgcgcagaa
     5161 gctgggcgaa ctggggcagc cggtgctggt gcactgcgaa aacgcactga tctgtgatgc
     5221 actgggcgaa gaagcgaaaa gtgaaggtcg cgtaactgcc catgactatg tggcttcgcg
     5281 tccggtattt accgaagtgg aagcgattcg ccgcgtactg tacctggcga aagttgccgg
     5341 ttgccgtctg cacatttgcc atatcagcag cccggaaggc gttgaggaag tgactcgtgc
     5401 acgtcaggaa ggtcaggatg ttacctgtga atcctgcccg cattactttg tactggatac
     5461 cgaacagttc gaagaaatcg gtactctggc gaagtgttca ccgccgatcc gcgatctgga
     5521 aaaccagaaa ggcatgtggg aaaaactatt taacggtgaa atcgactgcc tggtttccga
     5581 ccactcacca tgccctccgg aaatgaaagc cggcaacatc atggaagcat ggggcggtat
     5641 tgccggtctg caaaactgta tggacgtgat gttcgatgaa gcggtacaga aacgcggaat
     5701 gtctctgcca atgttcggca aattaatggc gactaacgca gcagatattt tcggtctgca
     5761 gcaaaaaggc cgtatcgccc caggaaaaga tgccgacttc gtcttcattc agccgaatag
     5821 cagctatgtt cttaccaatg acgatctgga atatcgccac aaagtcagcc cgtatgttgg
     5881 ccgtaccatt ggcgcgcgta tcacgaaaac catcttacgt ggtgatgtga tttacgacat
     5941 tgaacagggc ttccctgttg cgccgaaagg tcaatttatc cttaaacatc agcagtaatc
     6001 tggcccctgc aatgcccgtc cttgcggcgg gcattctccg gttaaggtgt gtttatgttc
     6061 aattttgcag tcagccgcga aagcctgtta tcaggatttc agtggttttt ctttattttt
     6121 tgcaacactg ttgtggttcc tcctacgcta ctttctgctt ttcagttgcc gcaaagcagc
     6181 ctgcttacgc tcacgcaata tgcttttctt gctaccgcac tggcctgctt cgctcaggcg
     6241 ttctgcggtc atcgtcgcgc tattatggaa gggccgggtg gcctgtggtg gggaaccatc
     6301 cttactatca cccttggcga agcatcgcgc gggacaccga tcaacgatat cgctaccagt
     6361 ctggcggtgg ggattgcgct ctccggcgtg ctgacgatgt tgattggttt tagcggatta
     6421 ggtcatcgcc tggcgcgatt atttacgccg tcggtgatgg tcctgtttat gttgatgctg
     6481 ggcgcacagc tgaccactat ctttttcaaa ggtatgctcg ggctgccgtt tggcatagcc
     6541 gacccgaatt ttaaaattca gttaccgccg ttcgcgctct cggtggcggt gatgtgcctg
     6601 gtactggcga tgattatttt cctgccgcaa cgttttgccc gttacggcct gctggtcggc
     6661 acgataaccg gctggttgtt gtggtacttt tgctttcctt cttcgcattc gctctccggt
     6721 gagttgcact ggcagtggtt cccgctcggc agtggaggtg ctttgtcgcc ggggattatt
     6781 ctgacggcgg tgattacagg tctggttaat atcagcaata cctacggtgc gattcggggc
     6841 acggatgttt tttatccgca gcagggcgcg gggaatacgc gttatcgtcg tagctttgtg
     6901 gcgaccggat ttatgacgct gataaccgta ccgctggcgg taattccatt ttcaccgttt
     6961 gtttcatcca ttggtttatt aacccagact ggcgattaca cgcggcgttc gtttatttat
     7021 ggcagcgtta tttgcctgct ggttgcactg gttccggcac tcactcgact gttttgcagt
     7081 atccctttac ccgtgagtag cgcggttatg ctggtttctt atctgccttt actcttttcc
     7141 gcgctggtgt ttagccagca aatcacgttt accgctcgca atatttatcg actcgcattg
     7201 ccgttatttg tcggcatatt tttaatggca ttaccgcctg tgtatctgca agaccttcca
     7261 ttaacgcttc gtcctctgct cagtaacggc ttactggtcg ggattttact ggctgttctt
     7321 atggataatc ttattccgtg ggaacgcatc gaataatttg ttgaaaaagg attgataatg
     7381 aagattgtca ttgcgccaga ctcgtttaaa gagagcttaa gtgcagaaaa atgttgtcag
     7441 gcaattaaag ccgggttttc gaccctcttt cccgatgcga actatatctg tttgccgata
     7501 gcggatggcg gcgaagggac ggtggatgcg atggtcgccg cgacgggcgg caacatcgtg
     7561 acgcttgaag tctgcgggcc gatgggcgaa aaagtgaatg ctttttatgg ccttaccggc
     7621 gacgggaaaa cagcggtgat tgagatggcg gcagcaagtg gtctgatgct ggtcgcgcct
     7681 gaaaagcgta atccgttgct ggcctccagt tttggtacgg gggagttaat tcgtcatgcg
     7741 ctggataacg gcattcgcca tattattctc ggcataggcg gcagtgcgac ggtcgacggt
     7801 ggtatgggca tggcgcaggc gcttggtgtg cgtttccttg atgccgacgg tcaggtgctg
     7861 gcggctaacg gcggcaattt agcacgcgtg gcaagtattg agatggatga atgcgatccg
     7921 cgtctggcga actgccatat tgaagtagca tgtgatgttg ataacccgct ggtggggcca
     7981 cgcggtgcgg cggcggtgtt tggtccgcaa aaaggggcaa cgccggaaat ggtcgaagag
     8041 ctggagcagg gactgcaaaa ttacgcccgt gttttacaac agcaaactga aatcaacgtc
     8101 tgccagatgg cgggcggcgg cgcggcgggc gggatgggca ttgcggcggc ggtattcctc
     8161 aatgcggata ttaaaccggg tattgagatt gtactgcggg cggtcaacct ggagcaggcg
     8221 gtgcagggcg cagcgctggt gatcaccggg gaagggcgca tcgactcgca aacggcaggc
     8281 ggtaaagcac cgctgggcgt ggcgtcggtg gcgaagcagt ttaatgtccc ggtgattggg
     8341 attgctggcg tattgggtga tggcgtggaa gtggtgcacc agtacggcat tgacgcggtg
     8401 ttcagcattt tgccacgtct ggcaccttta gccgaagtgc tcgccagcgg tgaaaccaat
     8461 ctcttcaaca gcgcacgaaa tattgcctgc gccattaaaa taggtcaggg aattaaaaac
     8521 tgactcttgc cattagagcg gatgcatttt atcccgcatc cgttttttac ctgaatttac
     8581 cgcaaaaaaa gccacggtat aaaccgtggc aaaatccaac atagctaaaa ataatcaggc
     8641 gagtggtatg acttaaatct ctacgtcgcg gttgcaatct ttcgagtaaa tataactaaa
     8701 cgcttcacca cgccctacgc cataaccggc ctgtaaggaa taagcgccca tgaagatgta
     8761 atcgcctttt ttcaccggga tccagttatt gtcgaggtta taaaccccct gaccggaaag
     8821 aatataggca ccgtgttcct ggacgtgagt ttcgatataa ccgtggctgg cacctggcgc
     8881 aaaagagagg atatgcatgt tcatatcaaa acctaactct ttgggcagaa aatctagcag
     8941 aataacatcg tccatgcctt cataatgaat gcgttcgagt tcgctggcat tgccagaaac
     9001 cagccacggt gcatggcctt ctaccggaac atagcggcgc ttatataaaa agatttggct
     9061 gtcttcggcc tgggcgttaa caaacgtcat taaggagcct ggtgggcaat aaagatagcc
     9121 accttcgctt aaggcaaatg ttttgccttc agctttggca gtgatatttc cagagatcac
     9181 atacaggaac gtttcaatgc cttcgccacc gaagccctgt tggttgccac cgttttgatg
     9241 cagtgtgacc agataatcaa caaaagaggc acccagcttt ggcgtggaga ggattgtcgc
     9301 gtcacaattt tcaaagcccg gaataatatt ttttaccaga ccatccgggg ttaaaagtgc
     9361 gaaattaccg tgtttaacaa tcgcacggtt agccagtaaa tcatcgcggt aaccggtgac
     9421 gttatttaaa tatcccattt atgactcctt atttctgcca ggcaagttga taaagcatga
     9481 gtgccaacgt tttgacccct tcggcaaggt cggtaatatt ggtgcgttcc gccgggttat
     9541 ggctgatccc gttgatgctg gggataaaaa tcatgcaggt tggtacgcga ggcgcgaaaa
     9601 tttgcgcgtc gtgcccggca ccactgtgca tcacccggta attcagtttt tcactttcac
     9661 acaattctgt cagggtggcg accagttcct tgttcatcgg cacgggttct tcgtccatcc
     9721 ataaatcgat atcaatacca atgtccattt catcgcaaat cgcccgcatg tcgttttcta
     9781 actgttgggt gaaatcgcgc agcacggcag catcggtatg gcgacaatca atggtgaacg
     9841 tggttttacc cggcaccaca ttcaccgtat tcgggcgcgg ctccacctta ccaaaggtca
     9901 gcaccagcgg gtcgcccatc tttttcgctt tttcgacaga ctgatggcaa atgcgactga
     9961 aagcgtaaac cgtatcacga cgataaccca tcggcgtggt gcctgcatgg tttgattcgc
    10021 cgttcagcgt caccgtatag cgacgctgcc cgacaattgc attcaccacg ccaattgatt
    10081 gaccattact ttccagcaca cagccctgtt cgatatgcag ttcgacaaag gctttaatat
    10141 cctgacgcgg agttagtggg gcgttcggca gagtaaatcc gcaagccttc atcgcatcga
    10201 caaaactatt tcctttggca tcacagatat tccgcacgtc gtcaggattc gccagcccaa
    10261 agatattttt actgccccag aagacatacg ggaagcggct gccttcttct tctgccatcg
    10321 ccaccacttc gaccgtacgt aacggcgcgc cgtattgcgt tttcagccag tcaattgcca
    10381 gccacgccgc cagcgcgccg aattgcccgt cgaggttacc gccgttaacc acggtatcga
    10441 tatgcgaacc gctcagaacc acttcttgtg gatattcggt gccactcagg cgaccatata
    10501 aattccccac ttcatcgaaa cgtgtttcca gcccgcttgc tgccattctt tttttaaatt
    10561 gctgctgagt ttccagccat tccggcgaat aaagtaaacg ggtcatccca cccgttgggt
    10621 cagcgccaaa agaggaaagc cagggcagcg tttcttctat ggcttgacgg aaatgtgtaa
    10681 tcataagaaa gtcctgtctc aataattatt gcgcaaaggg atttttcgtt tcgtatgacg
    10741 tgttataaag cgcgtcagaa attaaatact ggtaaatatc atcaacaatt tcgatgcctt
    10801 cgacggcggc tttgcgttgt ttaatatcct gatcctgtcc gggataataa acctggttaa
    10861 aaccgggcgc gggggttatg gcatttaatt cgcgcatggt ctggctaaga tgttgacgga
    10921 atagttcgct ggaggagaaa aagttcgggt taataacgat atgtaattgc cccaaattac
    10981 gcccggcgtg taaatcgtca tacatcgaac taacctgtcg cccgaacggt aagccgagta
    11041 agacgcctga gaggacgtca atcatcatca tcaggccata ccctttcggt ccggcggcag
    11101 ggagcagggc gttaactgca aatggatcgg ttgttggtgc tccgttttta tcgaccgccc
    11161 aggtatccgg gatagacata ttacgcgagc gggcgtcgag cacttttccc catgcctgta
    11221 cggtagtcgc catatcaaag gtaaggatct cgtcgccttc tcccggcgcg gcaaaggcca
    11281 gtgggttagt accgtagtaa atttccgcgc cgccaaacgg caccaccatt ggatcggact
    11341 ggcacatcga aatgccaatt aatcctgcgc gggctgcctg ctgcacaaaa taagagattg
    11401 cgccgctgtg acccatccgg ctgataccga ccaccgcaac gccattttgc tgggcggttt
    11461 tgatggcatg ttccataccc attttcgccg cgacctgtcc ggcagcattg tcggcatgta
    11521 aaattgccga acacggcccg gtttcttcaa gacgaaattc cggttcacgg ttggtgccgc
    11581 cttttgaaat gcgttccgcg tagtattcca cgcgcaccgc gccatgagag tggatccctc
    11641 tggcatcggc gtaaaccaat acttcagcca cggttgcagc gtgctcacgt tttaacccag
    11701 cctggcagag tttattctcg attagctggc ggagtgtttc ccgactgatt ttcatctgtc
    11761 ttccttttta acgacggtgt gaagcatgac tgcaattaac atacagggaa aatatctgga
    11821 ttatgtgatc cagacaggca aaaaaatata gttagaattt atttgataat ccgctcgctt
    11881 ttaacctggt ttttaaaaca accacgctta ttaaaaaata acacgtaata gcatggcggt
    11941 tatttgaatt cttttgttaa taatccctgt gtgatattca tcaccttatt ttcccgttct
    12001 catcgatacc gtaatcgcca cattaacact gctcgtgcaa ttgccatggg tgcaattttt
    12061 aaggagttgt tatgatccac gcctttatta aaaaagggtg ttttcaggat tctgtcagtt
    12121 taatgattat ttcacgaaaa ctcagcgaat cagaaaatgt tgatgatgtt tccgtaatga
    12181 tgggtacgcc cgccaataaa gcgttattag ataccacagg tttctggcat gacgatttta
    12241 ataacgccac gccgaacgat atttgcgtgg caattcgtag cgaagcggcg gatgcgggga
    12301 tcgcgcaggc gattatgcaa cagcttgaag aggcgctaaa acaactggcg cagggttcag
    12361 gcagcagcca ggcgttgacg caggtgcgtc gctgggacag tgcctgtcag aaattacccg
    12421 atgccagtct ggcgctgatt tcagtggctg gcgagtatgc ggcggagctg gctaatcagg
    12481 cgctggatcg caacctcaac gtgatgatgt tctccgataa cgtcacgctg gaagatgaaa
    12541 tccagctcaa aacccgcgca cgcgataaag gcttgctggt gatggggccg gactgcggta
    12601 cgtcgatgat tgccgccacg ccgctggctt ttgctaacgt gatgccggaa ggcaatattg
    12661 gcgtcattgg cgcttccggt accgggattc aggagctgtg ttcgcagatt gcgctggcag
    12721 gtgagggaat tacccacgcg attggtcttg gcgggcgcga cctcagccgt gaagtgggcg
    12781 gcatcagtgc gctaacggcg ctggaaatgt taagcgccga cgcgaaaagc gaagtgctgg
    12841 catttgtttc aaaaccacct gccgaagctg tgcgtctgaa aattgttaat gccatgaaag
    12901 caaccggcaa accgacggtg gcgctgtttt taggttatac cccggcggtg gcccgcgacg
    12961 agaatgtctg gtttgcctcc tcgcttgatg aggccgcacg cctggcttgc ctgctttcac
    13021 gcgtcacggc gcgacgtaac gcaatagcac ctgtcagcag cggatttatt tgcggtttgt
    13081 ataccggcgg tacgctggct gctgaagcgg cgggattact tgccggacac cttggcgtgg
    13141 aagccgacga tacccatcaa catggcatga tgctcgacgc cgacggacat cagatcctcg
    13201 acctgggcga tgatttttac accgtcgggc gtccccatcc gatgatcgac ccgaccttac
    13261 gcaacctgtt aattgccgat ctcggcgcta aaccgcaagt gcgcgtgttg ctgcttgatg
    13321 tcgtgattgg cttcggtgcg actgccgatc ctgccgcctc gctggtgagc gcctggcaaa
    13381 aagcctgtgc cgcgcgtcca gacaatcaac cactgtatgc cattgccacg gtgacaggca
    13441 ccgaacgtga cccgcaatgc cgctcgcaac aaatcgccac gctggaagat gcggggatcg
    13501 cggtcgtgag ttcgctgccg gaagccacct tgctggcggc agcgttaatt catccgctct
    13561 cgcctgccac acagcaacac acaccgtcat tactggaaaa cgtcgccgtg attaacatcg
    13621 gattacgcag ctttgcgctg gcgctacaaa gcgccagcaa accggttgtg cattaccaat
    13681 ggtcgccagt cgccggcggc aataaaaaac tggctcgttt attagaacgt ttgcaataag
    13741 gggttcccat gtttacatca gtggcgcaag ccaatgctgc ggttatcgaa caaattcgtc
    13801 gtgctcgtcc acactggctg gatgtgcaac cggcttcttc acttatcagc gaactaaacg
    13861 agggcaaaac actgcttcac gccgggccgc caatgtgctg gcaggagatg accggaccca
    13921 tgaaaggggc atgtgtgggc gcatgtctgt ttgaaggctg ggcgaaagat gaagcgcagg
    13981 cgctggcaat gctggatcag ggggaagtga acttcattcc ttgtcaccat gtgaatgccg
    14041 tcgggccaat gggcggcatt acttctgcca gtatgccgat gctggtggtc gagaacgtga
    14101 ccgacggcaa ccgggcgtac tgcaacctca acgaaggtat cggcaaagtg atgcgttttg
    14161 gcgcttacgg cgaagatgtc ctgactcgcc atcgctggat gcgcgatgtg ttaatgccag
    14221 tattaagcgc ggcgctgggg cgcatggagc gcggtatcga tctcaccgcg atgatggcgc
    14281 agggcattac catgggcgat gagttccatc aacgcaatat tgcttcctca gcactgttaa
    14341 tgcgtacgct ggccccacaa attgctcgcc tcgatcatga taaacagcac attgcggaag
    14401 tgatggattt cctcagcgtg accgatcagt tcttcctcaa cctcgcgatg gcctactgca
    14461 aggcggcgat ggatgctggc gcgatgatcc gctcaggcag catcgtcacg gctatgaccc
    14521 gcaacggcaa tatgttcggg attcgggtca gcgggctggg cgaacgctgg tttactgcgc
    14581 ctgtaaacac tccccaaggt ctgtttttca ccggtttctc gcaggagcag gcgaacccgg
    14641 atatgggcga tagcgcaatt accgaaacct ttggtattgg cggagcggcg atgatcgcag
    14701 cgcctggcgt aacgcgcttt gtcggtgcgg gtggcatgga agcggccaga gcggtatctg
    14761 aagagatggc ggaaatcttc cttgaacgca atatgcagtt gcagatcccc ggctgggatt
    14821 ttcagggcgc gtgcctgggg ctggatattc gtcgcgtggt agaaaccggc attacgccac
    14881 tcatcaatac cggtatcgcc cataaagagg cggggatcgg gcagattggc gcaggcactg
    14941 tgcgggcacc gctggcgtgc tttgaacagg cgctggaagc actggctgaa agcatgggga
    15001 ttggttgagg aacgcacaat gacgatcatc catcctctgc ttgccagccg tagtgcaccg
    15061 aattatcgcc agtcctggcg gttagcgggc gtgtggcggc gggcgattaa cctgatgacg
    15121 gaaagcggtg aattgttaac actgcatcgt cagggtagtg gtttcggccc cggaggatgg
    15181 gtgcttcgcc gcgcgcaatt cgatgcgtta tgcggtggat tatgcggcaa tgaacaaccg
    15241 caggttgtgg cacaagggat tcgcctcgga cgtttcacgg ttaaacagcc acagcgttat
    15301 tgtttgctgc gtattacgcc gcctgcgcat cctcaaccgc ttgcagctgc atggatgcaa
    15361 cgcgcggagg aaaccgggct tttcgggcca ctggcgttgg cggcaggcga tccgcttcct
    15421 gctgaattac gccagtttcg ccactgtttt caggccgcgc tcaatggcgt gaagaccgac
    15481 tggcggcact ggctgggcaa aggtccggga ttaacgccga gtcatgatga cacgctgagc
    15541 ggaatgctgc tggcggcctg gtattatggc gctttagata cgcgctccgg tcgccagttt
    15601 tttgcctgtt ccgacaatct tcaactcgtt accacagcgg tgagcgtcag ttatttacgt
    15661 tatgccgcgc aaggatattt cgcctcgcca ctcctgcact ttgtccatgc tctgagttgc
    15721 ccgaaacgta ccgctgttgc gattgattcg ctgctggcgc tggggcatac gtcaggggca
    15781 gatacgctgc tggggttctg gcttggtcaa caattattac aaggaaaacc atgaaaacac
    15841 tggttgtggc tcttgggggc aacgccttac tccagcgcgg tgaggcgctg acggcggaaa
    15901 atcaatatcg caatatcgcc agtgctgtac ccgcgctggc acgcctggcc cgttcttatc
    15961 ggctggcgat tgttcacggc aacgggccgc aggtggggct gctggcattg cagaatctgg
    16021 cgtggaaaga agtggaaccg tatccgctgg atgtgctggt ggcggaaagc caggggatga
    16081 ttggctatat gctggcgcag agcttgagcg cacagccgca gatgccgcct gtcacgacgg
    16141 tgctgacgcg cattgaagtt tcgcctgatg atccggcatt tttacagcca gagaagttta
    16201 tcggtccggt ttatcagcca gaagaacaaa aggcactgga agcggcttac ggctggcaga
    16261 tgaaacgtga tggtaaatac ttgcgccggg tggtggcgtc tccgcaaccg cgtaaaattc
    16321 tcgacagcga agccatcgag ttgttgctca aagaggggca tgtggtgatt tgcagtggtg
    16381 gtggcggtgt gcctgtgacg gacgacggag cagggagcga agcagtgatt gataaagatc
    16441 tcgccgctgc gttgctcgcc gagcagatta acgccgatgg gctggtgatc ctcaccgatg
    16501 ctgacgcggt ttatgaaaac tggggaacgc cgcagcaacg cgccattcgc cgtgccacac
    16561 cggatgagtt agcgccattt gccaaagccg atggttcgat ggggccgaag gtaacggcgg
    16621 tgagtggcta tgtcagaagc cgtggcaaac ctgcgtggat tggggcgtta tcgcggattg
    16681 aagagacgct ggcgggcgaa gcggggacct gtatttcgct gtagtcgtag gcattagaca
    16741 tttgtgcctg atgcgacgct tgacgcgtct tatcaggcct acaatgggta ccggagcgtt
    16801 aagccggata aggcgttcac gccgaatccg gtaagaatat gtgcaccagg ttaactgaac
    16861 ttactctgcg cccaaatcac gccgctggca tactctggcg gcagcagcgg gattaaggct
    16921 tccagcgtcg cggtcagacg cgatgtgtcg ctgtcggtca aattcagatg ccccacttta
    16981 cggcccggac ggacttcttt gtcgtaccag tgcagatgca ccagcggcaa tttcagccag
    17041 tcataattca catcgctacc aatcaggttg atcatcaccg acggactatt cactaccggt
    17101 tgcggtaacg gcagatcggt aatcgcccgc agatgcagct caaactggct gatgctggca
    17161 ccgttttgtg tccagtgacc gctgttatgc acacgcggag ccagttcgtt gatcagcaga
    17221 ccttgcgggg tgacaaaaca ctccatcgcc atcacgccca catagcccag ctcctgcata
    17281 atcgccgaca gcatctcttc ggcttgcgct tgctgctgcg cattggcctg cgggaaagcg
    17341 acgctggtac gcaaaatacc gtcctgatgc aggttatgcg tcagcggata aaacacggtg
    17401 ctaccatcaa agccgcgcgc gccaaccagc gacacttcgc cagagaagtt aatgccctgc
    17461 tcgacaatac attcgccgta acactctgcc ggtaactgtt cggtttcatc ggcacgtaaa
    17521 cgccattgac cgcgaccgtc atagccacca gtgcgacgct taacaatcgc cagctcacct
    17581 aaacgatcaa acaccgcagg ccactcgctg cgatcggcaa gtaactgcca cggtgcggtc
    17641 ggcaggtgga gcttatcgaa aagctgtttc tgagtcagac ggtcggcaat aatcgggaac
    17701 acatcgcggt tcacaaaggc cggatggcgc gccagttcgc gggttaatgc ggtttccggc
    17761 cagcgttcga tctcagcggt aatcacgctt tgttgaaaag gcaccgccgc cggttcagcg
    17821 tccagtccga ccggccagac agcaatgcct aacggttcgc ctgcctgacg cagcatacgg
    17881 cctaactgcc cgttaccgag gacgcaaacc tgtttcatgc cgcacctcgc gggtccgggt
    17941 tttccagcac ttcatcagtt tgtgctttgc gccagtcatt cagacgctgg tgcagttctt
    18001 tatcatgagt cgcaagaatt tgtgctgcca gtaatgccgc gtttgccgcg ccagctttac
    18061 caatcgccag cgtacccacc ggaatgccgc gtggcatttg tacgatggag tagaggctat
    18121 cgacaccgct cagtgcggcg ctctgtactg gcacgcccag caccggcacc agcgttttgg
    18181 cggcaatcat acctggcagg tgcgctgcgc cgcctgcgcc cgcaataatc acctgataac
    18241 cgttctcttc ggcgctttcg gcgaagctga acagtttatc gggggtgcgg tgagcagaaa
    18301 ccacttcaac gtggtgcggg acattcagga tttcgaagat ttcggcggcg aactgcatgg
    18361 tagcccagtc gcttttggac cccatcacga tggcgacacg cgccggatta ttgcgggaag
    18421 acatgcgtct taaaactcct gtggtgcaca actctcggct ttagagggca cagagaatag
    18481 cacggaaaga gagcaaggaa aacggttgcg tggctgtgaa atcagcaaag ttgcgggttt
    18541 tttaaaacgg aaaatgaatc agctcaacgt catccgccgt gactttcacc attgaacctt
    18601 cagtatgcca ggctcccagc acgacgcgga aagcaggttg ctgattggcg ataagttcat
    18661 gtactgccgg gcgatgggta tgcccgtgaa tcagccattg cacctgatgt ttttccatcg
    18721 cactgactac cgcgttttgg ttaacgtcca tgatcgccag cgatttactg ctgttggctt
    18781 ctttgctgtt cgctcgcatt cgcgcggcaa tgcgtttgcg cacaaacaac ggcagggcga
    18841 ggaatagcgt ttgcagccag ggtttgtgga ctttggcgcg aaaagcctga taaccggcgt
    18901 catcggtgca cagcgtgtcg ccatgcataa tcaatacccg gcgaccatac agttcgagca
    18961 ccttttcttc cggcaataac gtcatgccgc tttcacgggc aaagcgtttg ccgagcagaa
    19021 aatcacggtt gccatgaatg aaataacagg gaacaccgga atcggacacc gctttgatcg
    19081 ctgccgccat ctggcgatgg agtgggttgg gatcgtcgtc gccaatccac gcttcaaaca
    19141 gatcgccaag aatatacagc gcgtcggcct tgcgggcttc ccccgctaaa aaacgcagaa
    19201 aaccggcggt gatcgccggt tcttccgcgc agagatgaag atctgcaata aagagtgtcg
    19261 ccacgattac tcgctaacgg tcacgctttc aatgataacg tcttcttttg gcacgtcctg
    19321 gtgcataccg ctacgaccgg ttgcaacacc tttgattttg tctaccacgt ccatgccttc
    19381 aaccacttca gcaaacacgc agtagcccca accttgcagg ctttcgccag agaagttcag
    19441 gaagtcgtta tcaaccacgt tgatgaagaa ctgtgcagtt gcagagtgcg gtgcctgagt
    19501 acgggccatt gccagcgtac cacgggtatt tttcagaccg ttgttggctt cgtttttgat
    19561 cggttctttg gtggcttttt gtttcatacc cggttcaaaa ccgccgccct gaatcataaa
    19621 gccgttgata acacggtgga aaatggtgtt gttgtaaaaa ccttcgcggc agtagtccag
    19681 gaagttttta actgtttcag gtgctttatc gtcaaaagtt ttgatgacaa tatcgccgtg
    19741 attggtgtgg aaagtaacca tttttgcatc ctgttccgtt tgattggtgc ttcaacccgg
    19801 ttcgggtcat atatagggtg gtgttatagc ataaccgcac gatcggatca tcacgcaatg
    19861 tatgctgatt cgcgcgggaa atatgggtat tatacgcaac tcaattaccc acacatgtct
    19921 aaacggaatc ttcgatgcta aaaatcttca atactctgac acgccaaaaa gaggaattta
    19981 agcctattca cgccggggaa gtcggcatgt acgtgtgtgg aatcaccgtt tacgatctct
    20041 gtcatatcgg tcacgggcgt acctttgttg cctttgacgt ggttgcgcgc tatctgcgtt
    20101 tcctcggcta taagctgaag tatgtgcgca acattactga tatcgacgac aaaatcatca
    20161 aacgcgccaa tgaaaatggc gaaagctttg tggcgctggt ggatcgcatg atcgccgaaa
    20221 tgcacaaaga ttttgatgct ttgaacattc tgcgcccgga tatggagccg cgcgcgacgc
    20281 accatatcgc agaaattatt gaactcactg aacaactgat cgccaaaggt cacgcttatg
    20341 tggcggacaa cggcgacgtg atgtttgacg taccgaccga tccgacatat ggcgtgctgt
    20401 cgcgtcagga tctcgaccag ctgcaggcag gcgcgcgcgt tgacgtggtc gacgacaaac
    20461 gcaacccgat ggatttcgtt ctgtggaaga tgtcgaaaga gggcgaaccg agctggccgt
    20521 cgccgtgggg cccgggccgt ccaggctggc acattgaatg ttcggcaatg aactgcaagc
    20581 agctggggaa ccactttgat atccacggcg gcggttctga cctgatgttc ccacaccacg
    20641 aaaacgaaat cgctcagtcc acctgtgcgc atgatggtca gtatgtgaac tactggatgc
    20701 actccgggat ggtaatggtt gaccgcgaga agatgtccaa atcgctgggt aacttcttca
    20761 ccgtgcgcga tgtgctgaaa tactacgatg ccgaaaccgt gcgttacttc ctgatgtcgg
    20821 gccactatcg cagccagctg aactacagcg aagagaacct gaagcaggcg cgtgcggcgc
    20881 tggagcgtct ctacactgcg ctgcgcggca ccgataaaac cgttgcgcct gcgggtggcg
    20941 aagcgtttga agcgcgcttt attgaagcga tggacgacga tttcaacacc ccggaagcct
    21001 attccgtgct gtttgatatg gcgcgtgaag taaaccgtct gaaagcagaa gatatggcag
    21061 cggcaaacgc gatggcgtct catctgcgta aactttcctc cgtattgggc ctgctggagc
    21121 aagaaccgga agcgttcctg caaagcggcg cgcaggcaga cgacagcgaa gtggctgaga
    21181 ttgaagcgtt aattcaacag cgtctggatg cccgtaaagc gaaagactgg gcggcggcag
    21241 atgcggcgcg tgaccgtctt aacgagatgg ggatcgtgct ggaagatggc ccgcaaggga
    21301 ccacctggcg tcgtaagtaa ttgcgctatt gccggatgcg agttttcgca tccggttatc
    21361 gtctgcgcca ccacaacatt cccatcagta gcatccccgg caaccacacc cacatcaatt
    21421 cagaaataat cacctgatgc ccgtacggtg tgctgtaacg agacaatgca aacggcgcga
    21481 ctttaatcac ctgccaggga gcgaaaaagc gttcatctga ccacggccac agccagccaa
    21541 cccctttacc gccagtggtt accgaatcca gcaagctgtg cgatagcaac gagacggtta
    21601 aaaacagcca gtagcgaatc agcccagccc tgaaccatcg gcgtccaatc agcacacata
    21661 acagcgggac aacaaacgca aacaccagcg aatgggtaaa cccgcgatga ccaaaaacat
    21721 tgccgtaagc aacgccaaat ttaaacgaca agacgtcggc atcgggcagc atcgccagga
    21781 tgattccggc aaataacagg cgcggaggga tgactttcga acccaaccct aaaccaatgc
    21841 aaaggggaac ggcggcgtgc gtaataacgg ttggcatgat ggtcccttcg gcaaaatgtc
    21901 gatgctatca gcatggatga acggggcgta gagggcaaaa gtctgaaaag agaaccggcc
    21961 tgttgataca ggccggggaa gggatcaggc aacaacctgt acgctgtgac cggcaaaact
    22021 cactgtctga ccggcgacga ttttgcagcg tttgcgcgtt tcaaccgcac cgtcgacttt
    22081 tacctggcct tcggcaatcg cgattttcgc ctgcgcgccg ctttcgctcc agccttccag
    22141 tttcagcaag tcgcacagct caacgtgcgg atgtttacct aaagaaaatg tcgccatgtt
    22201 actcaccctg tggatcatga tattcaacgc acgcctgtag cgtgttttca atcagcgtgg
    22261 caaccgtcat cgggccaacg ccgccgggaa caggcgtaat gtatgaggcg cgtttagccg
    22321 cgtcttcaaa cacgacgtcg cccacaactt tgccattttc cagacggttg atgccgacat
    22381 caatcacaat tgcgccttct ttgatccagt caccgggaat aaagcctggc ttaccgacgg
    22441 caacgatcaa tagatcggca ttttctacgt ggtgacgcag atttttagtg aaacggtgag
    22501 tcacggtggt ggtgcaacct gccagtagca gttccatgct catcgggcgg ccaacgatat
    22561 tcgatgcgcc aatcaccacg gcgttgaggc cgaaggtatc aatgttgtaa cgctcaagca
    22621 gcgtgacaat accgcgcggg gtgcagggac gcagacgcgg cgcgcgctgg cacaggcgac
    22681 cgacgttata aggatggaaa ccgtctacgt ctttgtccgg gtgaattcgt tccagcactt
    22741 tgacgttatc aatacccgcc ggtaacggca gttgaaccag aatgccatcg atggtgttgt
    22801 cggcattcag cgtatcgata agctccagca gctccgcttc gctggtggtt tccgggaggt
    22861 cgtaagagcg ggagacgaac ccgacttctt cacaagcctt gcgtttgctt gcgacataaa
    22921 tttgcgatgc agggttacta cccaccagca caacggccag tcctggtgcc cgcagtccgg
    22981 ctgcaatacg cgcctgaact ttttgagcaa cttcagagcg cacctgctgc gcaatcgttt
    23041 taccgtcaat aatctttgct gccatcagag agaggattcc atctgttacg taggtcgaag
    23101 gggatgcgcc tattttgtca gaagcggggc gcgctgtcag gtttcgtttc agatttatcg
    23161 cgtgaagcga cctcgtgcga aggtgaggcg cactgtcgct gagattgaaa gcttcatttt
    23221 tcgtccatga tggcgttgta aatctggaac tgacttattt ccttgtcaaa ggattaagat
    23281 aatttaagaa atacctgaca atataaaaag aattttcggc ctg
//
LOCUS       DAYPHN010000068        23317 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000068 DAYPHN010000000
VERSION     DAYPHN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.068"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(11..1060)
                     /gene="yahK"
                     /locus_tag="ACG0BW_002510"
     CDS             complement(11..1060)
                     /gene="yahK"
                     /locus_tag="ACG0BW_002510"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFK7259460.1"
                     /translation="MKIKAVGAYSAKQPLESMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1147..2103)
                     /locus_tag="ACG0BW_002511"
     CDS             complement(1147..2103)
                     /locus_tag="ACG0BW_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7259461.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(2100..3071)
                     /locus_tag="ACG0BW_002512"
     CDS             complement(2100..3071)
                     /locus_tag="ACG0BW_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7259462.1"
                     /translation="MAELKKRHEFWLALLIVGLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(3064..4548)
                     /locus_tag="ACG0BW_002513"
     CDS             complement(3064..4548)
                     /locus_tag="ACG0BW_002513"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFK7259463.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSIPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(4597..5580)
                     /locus_tag="ACG0BW_002514"
     CDS             complement(4597..5580)
                     /locus_tag="ACG0BW_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7259464.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDIDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(5834..6244)
                     /locus_tag="ACG0BW_002515"
     CDS             complement(5834..6244)
                     /locus_tag="ACG0BW_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFK7259465.1"
                     /translation="MTIKEKISQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            complement(6539..7921)
                     /gene="yahJ"
                     /locus_tag="ACG0BW_002516"
     CDS             complement(6539..7921)
                     /gene="yahJ"
                     /locus_tag="ACG0BW_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFK7259466.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTAHQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(7931..8881)
                     /gene="yahI"
                     /locus_tag="ACG0BW_002517"
     CDS             complement(7931..8881)
                     /gene="yahI"
                     /locus_tag="ACG0BW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFK7259467.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMNRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(8977..10395)
                     /gene="yahG"
                     /locus_tag="ACG0BW_002518"
     CDS             complement(8977..10395)
                     /gene="yahG"
                     /locus_tag="ACG0BW_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFK7259468.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEISFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGIGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(10395..11942)
                     /gene="fdrA"
                     /locus_tag="ACG0BW_002519"
     CDS             complement(10395..11942)
                     /gene="fdrA"
                     /locus_tag="ACG0BW_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFK7259469.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDISRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(11932..12795)
                     /gene="yahE"
                     /locus_tag="ACG0BW_002520"
     CDS             complement(11932..12795)
                     /gene="yahE"
                     /locus_tag="ACG0BW_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFK7259470.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITIGQHLHIEMSHCLRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKALHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYRGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(12835..13440)
                     /gene="yahD"
                     /locus_tag="ACG0BW_002521"
     CDS             complement(12835..13440)
                     /gene="yahD"
                     /locus_tag="ACG0BW_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFK7259471.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            13698..14195
                     /gene="yahC"
                     /locus_tag="ACG0BW_002522"
     CDS             13698..14195
                     /gene="yahC"
                     /locus_tag="ACG0BW_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFK7259472.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            14287..15219
                     /gene="yahB"
                     /locus_tag="ACG0BW_002523"
     CDS             14287..15219
                     /gene="yahB"
                     /locus_tag="ACG0BW_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFK7259473.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMEQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(15261..16349)
                     /gene="pdeL"
                     /locus_tag="ACG0BW_002524"
     CDS             complement(15261..16349)
                     /gene="pdeL"
                     /locus_tag="ACG0BW_002524"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFK7259474.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(17226..19259)
                     /gene="betT"
                     /locus_tag="ACG0BW_002525"
     CDS             complement(17226..19259)
                     /gene="betT"
                     /locus_tag="ACG0BW_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFK7259475.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            19388..19975
                     /gene="betI"
                     /locus_tag="ACG0BW_002526"
     CDS             19388..19975
                     /gene="betI"
                     /locus_tag="ACG0BW_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFK7259476.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVDGNFDETQVS
                     NAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            19989..21461
                     /gene="betB"
                     /locus_tag="ACG0BW_002527"
     CDS             19989..21461
                     /gene="betB"
                     /locus_tag="ACG0BW_002527"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFK7259477.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNNELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            21475..23145
                     /gene="betA"
                     /locus_tag="ACG0BW_002528"
     CDS             21475..23145
                     /gene="betA"
                     /locus_tag="ACG0BW_002528"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFK7259478.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKDALPRSTAGYYVANGRPVRAKK"
BASE COUNT         5746 a         6337 c         5739 g         5495 t
ORIGIN      
        1 attaattttt tcagtctgtt agtgtgcgat tatcgataac aaaacgatat ttcacatcac
       61 cacgcagcat tcgctcatag gcttcattaa tttgatcggc ccgaatcatc tctatatcag
      121 ccacgatgcc atgttcggcg caaaaatcga gcatctcctg agtttctgga atgccgccaa
      181 tcatagaacc ggctatcgca cggcgtttca tgatcaggtt gaaaacttcc ggcgatttat
      241 gcggtgtcgc aggcgcacca accagcgtca tggtgccatc acgcttcagc aaggtggtaa
      301 aatcgtcgag attatgtggc gcagctactg tattcaaaat gaaatcgaaa ctcttgagat
      361 gagccgccat ctcatcggca ttgcgtgagt taacaacttc atcggccccc agggcttttg
      421 ccgcttcgcg ttttgactca gaagtggtaa atgccaccac atgcgccccc atcgcgtggg
      481 ccagcttaat ccccatatgt cccagaccgc cgatgccgac cacgcccact tttttacccg
      541 gcccggcctg ccagtgacgt agcggcgaat acgtggtgat ccctgcacac aacaaaggag
      601 ccaccgccgc cagctgctct tgcgggtgac gaatacgcag aacatatcgc tcatgaacga
      661 cgatctgttg tgagtagccg cccagagtat ggcccggttc gtccggcgtc ggcgagttat
      721 aggtgccggt catgtgatca cagtagtttt ccagcccgtc ttcacactct tcgcaatgtt
      781 tacaactgtc gacaatgcag ccgacaccga ccagatcgcc cggcgcatgt ttttctacct
      841 gatcaccaac ggctaccaca cgccccacaa tttcatgacc cggcacgcag gggtaaaccg
      901 tccccgccca ctcggaacgg acctggtgga gatcggaatg gcagacgcca cagtaagcga
      961 tttcaatttt gacatcatgc ggtcccggtt cacgccgggt gatatccatc gattcaagtg
     1021 gttgtttagc ggaatatgca ccaacagctt tgatcttcat tgtgtttact cctgattagc
     1081 tatgtgtatt tggcgatgcc tgatatgtca tcgcggcaaa acgcgtccat tgaatatagc
     1141 caatatttaa accacccgat ccagccccaa catgccgcga gcctgaatga aacagagcag
     1201 caacgttccc cataaggcca tcgtcaggta ggggcttata ccgagaatat tgaagccgct
     1261 ttcaataatt tgcagaagaa acagcgccag caccatacca attatccgcc cgctgccgcc
     1321 gtccggatta accccgccga gtactgccgc gaggattgac accagcaagt acgattcgcc
     1381 ataagaggcc ttagccgagt tgagtttcga catcatcaaa aatgccgcta ccgcacaaag
     1441 caaggcggaa atgacataaa cccaaatcag cacccgacga gtgttaatgc cggagtaaag
     1501 tgttgctcgt tcgttagaac caatcagata aatggattta ccgagcggcg ttttctccag
     1561 caatatccac agccccagcg ccactgcggc aaacaaccac atcggtagcg gaataccgaa
     1621 ccactgtgca tgattcaacc acagcaccca tgagggataa ttggcaatgg cgctaccgcc
     1681 tgtcaccaga atatttaccc ctttgagcaa cgtcatcatc ccgagtgttg caagaattgg
     1741 cgacactcga ataccagcaa tcaatacgcc gttacataaa ccgattatca ccgccgcgcc
     1801 agcgcccgcc agcagcgtag cgacaaccgt agcaataccc ggtggatact gagtcgccac
     1861 ccacgccatc accagtgaac aggcgttagc agtggctata attgacaagt taatgccacc
     1921 gcaaagcatg gtcaccgcca tagccaacgc cagaatgccc agcacgggca tttgcgaagc
     1981 gacggactgg aaattactta tcgaccagaa aatctccggc atagtaatgc tgaaggcggc
     2041 aacagttagc accaacaggc cgatcaagta aaactcaacg ttattacgcc acgatttttt
     2101 catagcagac tccggttctg atgctgactc cacgccgtgg cgctaatgct ggcaacgatg
     2161 atgatgcctg tgatcaatgt ttgccagtaa gacgagactc ccaataaatt tagcccgttt
     2221 tgcatcactg ccaacaacac cacgccgagc aaagtaccgg ttaacgtgcc gcgcccgccg
     2281 agcaaactag tgccgccaag caccaccgca gccagtactg tcagctcata acccagaaga
     2341 gaatcggggg cgacagtcat caccgtccac gactgcacta cacccgcagc accagacatc
     2401 aatcccatat agccgtagac gaaaagttgc agtttcagca cgctaaagcc gatgcgactg
     2461 gcggattcac ggttcccgcc aagggcataa attttgcgcc ctacactggt gaaattcatg
     2521 ataaacgctg ttagcagcac caccgtaatc atggcgatca gcggtagacc aaggccatag
     2581 tcatagccat cagcatcggt gtacttgaac aacataaccc cctgctcaaa ccacggcgga
     2641 aagtcgtaca gccacacacc tttactcaac cataacagca ggccatagaa aatattcagc
     2701 gtcgagatag tgatgataat agaaggcacc cgtaggcgat taaccagtaa ggcgttaatt
     2761 aagccaagta aaatgccgat gccgcccgcc agggcaaacg cgacagcgaa gttgccacca
     2821 attttttgca gcaacagcac catgccgtat tgcgcaatga tggtcattgc tggaaacgaa
     2881 atatcaattc caccggaaat caacaccaca aacaaaccac aggcgagaat agtcaacatg
     2941 gcatagttat tggcgagatc gtacaaatta ccgaatgtca gaaactcgtc gctgcgccag
     3001 gcaaggccga caaacagccc cacaatcagt aatgccagcc agaattcatg acgttttttc
     3061 agttcagcca tttaccacct ccgcaatcct ggcctgagaa ctgctgtcag gcaggaagct
     3121 atgggtgatc tgacctttct gcattaccaa aatccggtgg ctttgatacc acgcttcttc
     3181 aatttcatcg cagatcatca ataccgcaat gccatgagcc gccagatcgc tgataatgcc
     3241 gtatattcct gctttgttgg cgatatcaac accgacagtc gggctatcga gaatcaacag
     3301 tcgtggccca atcgccagcc atttggcgat cgatactcgc tgcgcgttac ccccagaaag
     3361 cgtattcacc gggagatgtg ggtcagagac tttgatggtc aatgccttga ttagcctgtc
     3421 caccaggtca cgcactttag cttcactcag aaagccgaag cgatctttta ccttgtgaaa
     3481 aacagtgcta atgatgttgt cttcaataga ttgcgccatc accaaaccac gcgacatgcg
     3541 atcttccgaa acataaccaa taccgtggcg gatggcgtcc tggttactat gcagtgtaac
     3601 cagctggccg ttgatgagga tctcgccagc atccggtcgg gtcataccaa acagactcag
     3661 acataattcc gtgcgccccg caccaagcag gccaacaatc gacaccactt cgcccgcttc
     3721 cacccgcaag ttaatatttt gatattcccc atgacgactt aaattacgca cctccagcac
     3781 tgggatgctg gattttccct gccacaactc tcgcacctga tattcaaact cctggccggt
     3841 catcaggaaa ccgagttgtt ttgtggtcat ctctgcagcc ggaaaggtcc cgaccagctc
     3901 accatctttc aacactgaaa tacggtcgga aacttccatc acctcttcca gacgatgact
     3961 gacgaagacc acgcagatcc cgcgttcccg caattgatgc accacctgta ataacccttg
     4021 cacttcctga tgggtcagcg aagcggttgg ttcatccatc acgatcaacc gtgcgtcctg
     4081 cgccagtgca cggcaaattg ccaccagctg acagcgggca attgaaagct cagaaacgag
     4141 cgtatccagt ggcaatgtga cgttaatact ggtcattgcc gcctgcgcca cctcacgcag
     4201 acgacggcgg ttaacaaaca gaccgtggtg atagtgattc acggcgatat tctcccagac
     4261 actcaggtta ggaaataaag agaggtcctg ataaatcacc tgaatcccct gcgccaccga
     4321 agcggcgggt gtcagctttg accagttttt cccaccaagc gtaatttccg ctccctcatc
     4381 cgggcgataa actccggaca taattttaat caacgtcgat ttaccacagc cgttttgccc
     4441 ggcaagacag tgcacttcac ccgacataaa gtcgagagaa acatcgcgca gcgctcgcgt
     4501 ggcatgaaac gttttgttga tatgacgaag ggaaaggaag gtttccataa catcccctgg
     4561 caataagaat cggggctttc gccccgaaga aatggattaa taaagtgagt cgatattgtc
     4621 tttgttgacc agcagtacct tatggaaacg gataattcgc ttatctatat ctacgtcggc
     4681 cttccccaga ttctgcattt ctaatccagg tttgatctct tcacccttca gcaacgtgct
     4741 ggcgacggcc gcgagtgcgt aacctgcaga ggccggatcg taagtaatcc cttcggtgat
     4801 atcaccactt ttaatcagtg atgccgcctg tgaagggatc atcatgccat agactgcgac
     4861 tttatttttc gcccgtttct ctttcactgc acgtcccgcg ccaatcggac cgtttgaacc
     4921 aaaggagaca accgctttca agtcaggata ggttttcatc aggtccagtg tggtacgacg
     4981 tgagtcatcc acactctcgg caaccggcat gcggcgggta acttcatgca tatccgggta
     5041 atgctctttc tggtatttca ccagcaaatc agcccataag ttatgctgcg gcacggtcaa
     5101 actacccacg taaatcacat agccgccctt gccacccatg cgtttcgcca tatgttcaac
     5161 atattcagcg gcaaattttt cgttatcaat gatttcgata tcccagttag cacttggctg
     5221 accgggggat tcgttggtca gaaccacaat tccggcatct cgcgcttttt tgaataccgg
     5281 ttccagcacg ttggcatcgt ttggcacaat tgtaattgca ttaaccttac gggcgattaa
     5341 atcctcaatg attttaacct gttgcggagc atcagtactt gaaggcccca cctgtgacgc
     5401 attaacacca aaggctttac ccgcctcgac cacaccttcg cccatgcgat taaaccacgg
     5461 cataccatcg actttagaga tattcaccac gactttttcc gctgcctgga gcggcgcaga
     5521 aattagcgca gcgcctaata acagcgaaga caccatattg ataacaaaac gtttattcat
     5581 catttcagcc tcattgcagg gtagtcagga agtgttcgcc caaattagcg cgctggagag
     5641 aataagaatt ttcgaatatt tttatgcaga ataaacagcc ctatttcagg cagctattaa
     5701 aaatttggtt ataaatatgg acaaaacggg cagcaataat aagtgtgaca ttcatcacgt
     5761 aaaaaatagc aaaataaaca aaattagcga tattttatgt ttgtgagctt atttcgcgta
     5821 gaaaaatagt tatctacaca acgtcaataa gggcatattc ttcaaagata acgtccattt
     5881 cgggtgaata gcatccttct gcctggaaaa aacgacggtg ttcattacgc caatattcaa
     5941 gactgaggtc accttcacct tccttacggg caagctccgc ggtgacatca gaaaagcgca
     6001 gtaaatgcag gccaatcact ctgataacac agacaggctc accacggcta ttttctacaa
     6061 tcttatactc accaatcatc ggaaatgcgt tgtcttcaat acatcccgcc agcgaaccac
     6121 aggaagctgt tttaacacct gtcgcaatca acgttgcgag gtgatcggcc aacgctgctg
     6181 aatcgccaaa agtacaaaag gaagcatggg gatatttttg tgagattttt tcttttatgg
     6241 tcatgagaat attttcgcga ttacggaaaa aaatcacatc ttaattactg ttgataacca
     6301 tcagcaagag gatcattggt ttttctatgt ttaatctttt ttatccgaag ccggtcacat
     6361 ttatggcttg ataaatattg ttaaaactca cttttgtgta tttctctcaa ataatcccct
     6421 ggaaattatg ccgggagatt ataaagttca ggatcattca ttaacaaacc attcccgccc
     6481 cactccctgg ggctttaaat ctcttacgat tcaccattgc aggcgtttca aatgcgtatc
     6541 aacctgccac actcccccac accagttgcc ctttatggaa cgtcgccgtt ctcggcgaga
     6601 tacgcgccac cgcctcggcg gaacaggagg cgtccaccag cacaaagctg gcatcatcct
     6661 gcgctttggg ccacacgcgc tcgccttttt cgttgagcgg caatacatcg ccagtggcta
     6721 aaaacagcga gcgggagagg ttctgttcgt taggacgaat atagagctgc gcgtacagat
     6781 tggctttttc cagcatgtcg cccaggccgt aaggcgacca gtggtcgata acgctgtcgg
     6841 tgccggtcat cacttttacg cctttgtcgt gcaactgttt gagcggcata tgcagcgtgc
     6901 caatcggcac cgtcgaggcg atagaaattt gctgcgccgc catccggtgc gccagttcat
     6961 ctacctgttg ctcattgagc gttgccagcg caaaggcgtg gctgatggtc agcttgccct
     7021 tcagttgcgg cgttttctcc accgtttcaa ccatataatt gatggctgcc acgcctgccg
     7081 gagtggtttc gtgcaggtga atatcaacgc ctttgtcgta gtccagcgca atctggaaca
     7141 tggtatcgag ggatttttcc atcgcgccat caacactggt cggatccaga ccgccgacgt
     7201 aatgcgcccc cgcctgcatc gcttcacgca ttaagggttc cgatttcgac agcagcaaac
     7261 cgtgctgcgg gaaggcgaca atttcacact cgaagcccgc ctgacgtcgc gccagcaccg
     7321 cctgcaaatt ttgcagattt ttcaggccgg aaaccggttc gatattgcag tggctgcggg
     7381 caatggtagt gcctttcgac tgcaataaat cgatcagttt ttccgctcgt tcctgagtgt
     7441 acggttgcag ctccggcagc attttctgct cgagtttgat catgtcctgg atggtggtgc
     7501 ctgccgggcg attgagcgag cgccacggcc cgccgtaaaa ggttttgtcg agatgaatat
     7561 gcatgtcgcg ggtggtgggc agcatcagct taccgccagc gtcatagtgc ggcagcgtgg
     7621 cgtccggatg ctgcttgttc tcgcgcaggg caacaatttt gccgttctga atctctacgg
     7681 tctggtgcgc cgtgcgggtc tgtaccgcca caccgttttc gtagtcaaaa ccggtttcca
     7741 gcagcacgtt gtcgagatag tagtgcgggt cagtgatttt catggtgtta ttcgcgtcgc
     7801 aatggattgt gccagagact gccgcatggg cgagcggcac agacgtacca aacagcgcgg
     7861 cggcggtgac catcttaccg ctctggctca gaaattcacg gcggctattg ttttctttca
     7921 tcttacgtcc ttacgtttta ataatatggg tgcccgtttc accgcgcagc gccgcaggca
     7981 ggcattccgg cgtggtgata atcacttctt tgccgccctg ttccaggaac gtcaggctgg
     8041 cgatgatttt tggcaacatg ctgccaggcg gaaaatgccc ttcctgcata tagcggttca
     8101 tggtggcaat atctacgcga tccagcgcct gctgctgcgg tttgccaaag tgaatacaca
     8161 ctttttcgac gccagtggtg atcaccagaa tgtcagcgtg aatttcacgg gccaatagcg
     8221 cggtagagag atctttgtcg ataaccgcgt ccacgctttg gtaatccccc gcttcactgc
     8281 acaccaccgg gattccgccg ccgcccgcgc caatcaccac aaagccttgt tggatcagcg
     8341 ccttaatggc cggtgcttcg acaatacgtt tcggttccgg cgaggcgacc acgcggcgat
     8401 agccccgtcc ggcatcttca acaaaacgcc agtcagggtt tgccttttgt aattcgtcac
     8461 gctggctttc actaaagaat gcgccgatgg gcttggtggg atgggcaaac cccggatcgt
     8521 ttttatccac ttccacctga gtcaccacgg tgacggcttt tttctcaccg tgacgcgcca
     8581 gtcggttatt cagcgcctgt tggatcagat agccaatgcc gccttgcgta tccgccacac
     8641 agttcgccag cggcgttaag ggcagccctt cgcgctcgtg ggctatctcc gcgcggcgta
     8701 aatccagccc gacctgcggc ccgttgccgt gggtcagcac aatgtcgtaa tcggaagcca
     8761 gcatttccag caccgtatca gcgacggctt tcaccgcctc cgcctgatgc tcaatcgact
     8821 ggctggcgtt atctttgata atgctgttgc caccaatggc aacgaccaca agttctttca
     8881 tgttgttatg tccgctacag agggtgatgg ggttatgaga gtgaggttgc ccctctcccc
     8941 tgaagggaga agggatcgta aggtgcggtt ggagagtcaa acgccgtaac gttcgcacca
     9001 gccaagaatg gctttctcga agcaggcgaa tggtggatgc acaatgcccg cgccaatcat
     9061 gccgatgccc gcatctttat gagcgatagc ggtgttgatc accggcagaa tgccactgct
     9121 acccacgcgg gtgatgtcga ttgccgatgg cacgcccata aaaccgagca gcggaatggt
     9181 gacgttgggg ttttcgccaa gggtgatttc acgcatctga cgggagaaat cgatagcctc
     9241 ttccaccgtg ccacccacca gcgcgacgat cgcgggcgcg gtcgccatag caaatccgcc
     9301 aatgccgtag gtttcggtga tggcgctgtc gccgatatcc agccccgaat cttccggctt
     9361 ataaccggca aacatcgggc cgatgacctg ctgcgccgga ccggtaaacc attgccccgg
     9421 caggccgctg acccgcaggc cgaactcgac gccgttacgc gccatggtgg tcaccacggt
     9481 gctgtattcg atgccgtgcg ccgcatccat cgccgcttta cacatcgcca tccacgtcgg
     9541 gccggagaag tagtcgctgc tggcgacaaa ctcaaacact tcgcgctgtt gctcgacgga
     9601 ataacccgcc tgaataatcc ccggcgtcag cgcctgaatc agcagcgtcg tcccggcgtt
     9661 attgcggtta tggcattcat cgcccatatg cagcgcttgc gccagcatta agcgcagatc
     9721 gatctcgccg ataattttca tcgcgtcgcg cagcattggc ccctgcacat cgcgcatcca
     9781 gttgaggcgg tcaatcacgc tctggtcgtt agcgcccata cgcaaaatct tcgccatctg
     9841 ctcgctcata ttggtgtaag cgatgttgcc gtaggttttg ttttttacga tgtgcataaa
     9901 catcgaggcc gaggtaacac ccgccatcga tcccacgcag tcatgttcgt ggcacggcga
     9961 gaaggagatc tccccggagg ccgccagttc agccgcctcg tcgagatctt tcgccagccc
    10021 ttcgaacacc agcgccccgg tgaccgcgcc tttcatcgcg ccgcacattt tttcccaggt
    10081 gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg cccggcacca cgttaatcgc
    10141 ctgatcaaaa ccaatcagca ccggatgcga ctggataatg cgctccagcg cctgctggtt
    10201 agcggcggcg attttgtctg ccagcggcga atcggcaatg ttatccagcg cctgcaccac
    10261 ctgcatattg ccctgccccg gcggcgtcca gtcgagttgg gtcacttcta catgctgttt
    10321 tttcagatca tcgctaaaca tggcgatacc gacgttaata acgttcaacg gttggctaaa
    10381 cagtgactgg ctcatcaggc ttcctcccct ttgcagataa attcacgcgc cagcaatccg
    10441 gtattggtgc tactgctggc cagaatcact ccggcatcaa gcagcatctg gctttgttgt
    10501 tctaatgatg gcgtatcaag atcggtaccc agcacatagg cgagaatgat caactcacgt
    10561 ccttcggcag cggcgatcgc tttcgcttct ttgatcgcct cgatggtgga gccgaccgga
    10621 tcttcatgcg atccaaatcc gagcacaaaa tccatcacga tcaccgccac ttctggatcg
    10681 cgcgcttctt cgatcaagcg actgatgcgg ttggtcgggt caatcattgg atgcggcttg
    10741 ccgttggtga agtcgtcatc accaaagtcg aggaaggtgt gtttgatgct gcggctgata
    10801 tcttgcaggc ggaattccgg atcgggctga atgttgctgt agacatcgcc gtgtttttcc
    10861 atcaccgcga acatcgtttc gtcgcacagc gtgccgccgc agaacaggcc acgaatgtat
    10921 ttctgttgcg gttgcaggcg agcgcgcacg tcagcaatca atggctggtt cagggtatgc
    10981 aggtcgagat gttcctgttt cacgccggag agcattaccg ctttcagcgc cgcctcttta
    11041 ctgccgcggg caaactgtag cccctgctca tccaccggcg tttcgccacg accgaggaag
    11101 caaaccacca ctggcttgcg gcaggcgcgc gcgcgttcca gcactttgcg ggccaccgca
    11161 ggcgcaggcg gtttggagat aagcacaatg atttcagttt gcggatcgtt ttccagcatc
    11221 ccgatggcgt cgagcatcat caggccgccg attttctcgc tcaggtcgcg cccgccggtg
    11281 ccgatcagtt gcgaaacgcc gccgccaaat tcatggatac ggacgctcag ttcctggcta
    11341 ccggtgccgg atgcgccaac aatgccgatg ttgccgcgac gcacggcgtt accaaagcag
    11401 agcgccgcgc cgttgataat cgccgtaccg cagtctggcc ccatcatcag cagccctttt
    11461 tcgtgggcca actgtttgag cgccagttca tcttcaactg agacgttatc ggaaaacagc
    11521 atcacgttaa gatcgttttg cagcgcctga cgcgcttcgc gagcggcaaa cagaccgttg
    11581 accgaaatca ccgccaggtt actttccggg atatgctttt tggcgctggc aatagtggcg
    11641 taacgcgcct cgtgcgagcc actttgcgct ttggtgttga acagttcttc aatctccgcc
    11701 agcaactgct cgttgtccac acccgattta ccattgatga caatcatcag gtcgccgttt
    11761 ttcgcctgct ccagctccgg cgtcagcagt cctaaattct tcagcacgcc tttgttcatt
    11821 tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga gtttatttgc acgcgtggag
    11881 atagacatca gcgagacaga atcaaaatag gtattcggtt taatgactat tttaactgac
    11941 ataattccct ccgtaggttt ggctcagcgc acaaccatcg gccatgccat acagcatgtc
    12001 gcagccggaa ctggagccaa tatgtttaat tttttctatt gcctgagttg cgttcccagg
    12061 gatgtcataa ataatgttat gaataaaacg atgaatattt tcccggcagc gttgttgtaa
    12121 tgcggcttcc agcgttatgg cacttaataa cgtggtatta tttctgccgc gttgcagacc
    12181 gcgataaaat tcctctttgt atttttctgc cggatgcccg gaaataaata aaataagcgc
    12241 cagacctgtt aaataatcat cggctgatgg cgtaaggcca atccctaagc ccatcatact
    12301 ggcgactgct gaggcgatat tttgtttatc gtttactgcc tgaataactg cccgacgttt
    12361 aatatgtagt gctttattta acgcctgata aaacggatta tcgccgcgat aattaaacag
    12421 ggtttcattt tcccccaggt gctgatgaat aatgtcatgc cactgttgcc aggcgataag
    12481 gtgaaaattc acagcggcca tttgcaaggt tggggacagc caacgcaggc aatgactcat
    12541 ctctatatga agatgttgac caatcgtaat gccttgatcg tcaaactgaa ccttatcacc
    12601 atgccggaac agaccatcaa agtgagtgag tgccaaccga caactgtttg gcgcattgtc
    12661 gtactcttca caaagcagcg tcagcaactg ctggcgggcc ggaagtgcga tatttaccgc
    12721 tctggcaaag acctgttcaa cctgtccttg cccccgctgc gccagaaaat cagcatccgc
    12781 agttagcgcc cacacacacc gtctttctgg tgtgttgata gcaagcctcc cggtttatgc
    12841 acctgcggca atcagtaact gcgcaatctc ttcaaagcct cgttcccgcg ccagttccag
    12901 cggcgttttg ccatatttat ccgtcatgtg cgggctggca ccgtgttcca gcaataactg
    12961 gacaatcgcc tgctgtttaa tcccgccatc attaagcaca attgcttcca gtagcggcgt
    13021 ccagccaaca tggttggtct ggttaacgtt aatctccgta tgcgccaaaa gctcttttac
    13081 aatacttaaa tggccttttt cacaggcagg cgtcaggccg acaccgccaa aacgggtgac
    13141 gcaattaaga tcaggtttag ccggtaaaat aatccgtaac agggttaaat cgccggtcag
    13201 gcagctaatt aaaaaaggat ttgaacatat ttgatcttgc ttattaatat ccgctccggc
    13261 atcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg taattgccgt
    13321 tttcccctga cgatcgcaag tattaatatc gacacccagc gcaaggcatg tttttacttt
    13381 atcgatatca ccctgttgcg cagccaataa atagtcggca ggtagatttt ttatagtcat
    13441 tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa cgataatctg
    13501 ataaaagaac ccgcttaaat ttaattcgcc gtatttcgcc tttttgttga atgatgttca
    13561 acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga taccctttca
    13621 ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttc gtccatgtct
    13681 aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc ggtatttgtg
    13741 cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg gaattattag
    13801 gaactattgg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcggt aaatcggggt
    13861 ttatcagaag cctggcggtt aattattctg ggatggtgtg ggcgtttttc gccgcgctgg
    13921 ctgccggttg gctggcctcg gtgagtggat tgtccacgtt ctgggcgagc gtgattatga
    13981 ctgtaccctt ctcggcggtg gtggtctggc aaggacgttt cggtttattg tcttttattc
    14041 ccggaggctt tctcggcatg acgctatttt ttgccagcgg gatgaactgg acggtgacat
    14101 tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggc ggacaaaaat
    14161 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
    14221 ctttaaccga tacctctaca gtcatggctg gagaaattca tattcgataa tggcgggaat
    14281 ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac cgctgcgcgt
    14341 tttggcagct tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc cattagctac
    14401 accatcaagc gaatggaaac agggctggat gtggtgctgt tcactcgcag tacccgcagc
    14461 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct gctgaatgat
    14521 tttcacgcca tcaaacgcag catcgatact atttcgcagg gtatagaagc acgggtgcgc
    14581 atttgtatta accaactgct ttatacgccc aaacacaccg cacgcttatt acaggtactg
    14641 aaaaagcagt ttccaacctg ccagattacc gtgacgaccg aagtgtataa cggcgtctgg
    14701 gatgcgatta ttaataatca ggcgaatatt gctatcggcg cacccgacac gctgctggac
    14761 ggtggcggca ttgattacac ggagattggc gcgattcgct gggcttttgc tatcgccccg
    14821 gatcacccgt tagcctttgt gccggaaccc atcgccgaaa gccagctgcg tctctatccc
    14881 aatatcatgg tggaagacac cgcgcatacg attaataaaa aagtgggctg gttgctgcat
    14941 gggcaggagt cgattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
    15001 gaaggaattg gttttttgcc ggattacatg gttcgcgagg cgatggagca atccctgctg
    15061 gttacccgcc aaatccataa tccgcgccag gattcacgca tgttactggc gacccagcac
    15121 tctgccaccg gtcaggtgac gcagtggatt aaaaaacaat tttcgccaaa tggcatatta
    15181 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
    15241 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
    15301 ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc tttgccgatc
    15361 attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag accaggctta
    15421 cgcgccagtt cgacaatatt gtccacaata tgaccggata tttcgtccac gctcgccatt
    15481 tgcacaaatg acttatcgat cttaataaaa tcgaccggga acgcctgcaa gtaacgatag
    15541 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
    15601 tcaaatatcg ctctggcttc tggcgttacc ggaatagggt tacgttccgt tagctcgaga
    15661 accagtttga ttttatcgtt tcctaatttc ttgaccaggt tcaggcattc tttttcaaat
    15721 cccgcggcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt atccggcagc
    15781 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcaactggcg agtcattatg
    15841 acgataagac cagatgactc cgccagagga ataaactgat ccggtgggat aattcccgtt
    15901 tgtggatgtt cccagcggac aagcacctca cagcccgtca gtaccccagt ctgcgcgcag
    15961 aaaaccggtt ggatccacgg tttaaactca tggttttcca acgccagact gatggcttca
    16021 ggcgtgacga tatggtgata gtgattatca ttaatatatt tgtgactatt attacccgtg
    16081 gcggatatga tctccggatt gtactgaaag aaaatatccc gccagaaagt aatatcgctc
    16141 tgaatcccca gtttctcaaa gagctgtttc ttttgatgtg aaatggtctt tgcactgcga
    16201 tttctgtact gtgatatttc tgttacagac attccctgca tcagcagctt gagtagcagt
    16261 cgttctttct cggatacgct accaggcaat gacaaatgaa ccgttgtacc gaactcttgc
    16321 agaaaaacac gaaaatcaca tgaattcatg aacacacctt tatcttttat cgctgttcag
    16381 tatgagtgat tacctgtgga ttcctgagca cacataaggg gtaaccataa acatcagcga
    16441 tgccatacgc ataacatccc acattactat caaaaagatt atctttacct ctgatgctcg
    16501 ctcaaaaaca gttacgctaa ataccttaaa aaggtaatca caagtcaaac tcaggagata
    16561 atcacgcaag aatgcaatat taaaattcag atactcattt ttggtaataa agaagtagtt
    16621 ggactatttc aatatttata atttcccact acgtattgac gcccattcat gagtaaaggc
    16681 gcattatatg aatttaacaa tccagatcaa ctgaaaatac ctcaacctca taaacacata
    16741 gcgtcaacat tcaatgacat aatgagtaaa gatgttggtt atgcatacac gtcattactc
    16801 tatgcctatc ccttaacaac ctgcttttta aggctgaatc cattcagcaa tagatagcca
    16861 acattataac aatgacaata aaaacaatat ttaaaacaat caataaaata tcagacacag
    16921 acaattaaac cttaaacaac aaacagttac attgatttta gttgatttgc gctacgcgtt
    16981 tcccatgaat aaagcgtcat ttcaaattag caccaatatt tacattaaat aagactaagc
    17041 ttattgaaca ttcacaacag tccaagcgta ggttttgggc ttcaatagtt cttttctttt
    17101 ttattatctg ttgcctggca atcatcaatc gatactaagg gcttaactgt acacagatat
    17161 aacgtatcaa gaggtaagag aaaccgcgcc atatgcagac atggcgcggt tttatgcaat
    17221 aacaatcacg cgtccgggaa catcacgcta tggcccggcg cttcacgatg gagatggata
    17281 aagttaagat gccgctcgta ctggtcaaga atatcggtga tcacctgctc tttgctgtag
    17341 tccatcaggt cgttgccctg gctgccttct aacaggaagg tttccagccg gtagtaggtc
    17401 gatttaccgc tacgtgcgcg gtaggtaaag cccggcaccg aatattgctg cggccaaatc
    17461 tgatagacaa agttttgctc ttcgcccata tgcaccaaca aatccagatg ccccaactgt
    17521 tgtccctctt ccggcggcag gctttttagc tccacgtacg cgccgcgcaa ccgcagctcc
    17581 tgcgccactt cttccattgc cgggtaacag accgtctcca tcatctgttt agtgtaacgc
    17641 gtgcccggat aattcatcag gcgcgagaga cgttttttcc agctcaggcg atcctgaagc
    17701 cccagcggtc gcggcgcggt atcacggttg gcactttcac ggcggtaatc ttctaccttc
    17761 agagatttat acaaccccgc catcacgaag aaaatcacaa agctgaacgg cagccccatg
    17821 attaccgtgg tgttttgcag cgcggatatc ccgttggtca tcagcatgcc gagcgttagc
    17881 aggccaatcg ccaccgacca gaagacgcgc agccagccgg gggcgtcgct gttgatatct
    17941 ttaagctgcg aggtgaaatt ccccagcacc agcgccccgg agtccgccga ggtcacataa
    18001 aacagcaggc cagtaatggt ggcgacggag gcgctaaagg taaacgccgg atactgcgcc
    18061 agcaggctgt agaagccgcg ctccggatgg accatcgctt cctcggcaaa tgccgcgccg
    18121 ccgtggatga tttcatacag cgcgctattg ccgaacaccg agagccataa cagcgtgaag
    18181 gtaaacggaa taatcaacgt gcccagcacg aactggcgaa tggtacgccc gcgcgagata
    18241 cgcgccagaa acaggccgac aaacggcgac catgccaccc accatgccca gaagaagagc
    18301 gtccagttat tcatccactc aaccggacgg tcgaaggcaa aactgttgag cgtcatgccc
    18361 ataaagcgat tcacatagtc gccaacgttc agcaccagcg cattaagcag gaacgaggtg
    18421 tcgcccataa acagcacgaa aaggatcaat cccagcgcca gcgcaacgtt aagttccgat
    18481 aacacacgaa tgcccttatc gacaccggag gtgacagaga tcgtggcgat gatcaccgac
    18541 aaggcgatca gtgccgcttt cgccgccatt gaatcgggaa tatcaaacag tacgctcaag
    18601 ccatagttaa gctgcaccac accgataccg agcgtagtgg caataccgaa gatggtgccg
    18661 attaccgctg caatatccac tgaatgacca atcggcccgt taatccgttt accgaagatc
    18721 gggtacagcg ccgagcggat ggtgagcggc aaattataac gatagctaaa gtatccgagc
    18781 gccatgccca tcagcgcata catcgaccag ccggttaagc cgtagtgaaa cagcgtccag
    18841 accatcgcct gacgcgcagc ctcaatcgtc tgacccgcgc cttccggcgg ctgcatatac
    18901 tgcgttaccg gttcggctac ggagaagaac atcaggtcga taccgatccc ggcagcaaac
    18961 agcatcgccg cccaactcag cagactgaat tccggtttgg attgttctgg cccgagcttc
    19021 accgaaccaa aacgcgaaca agcgatacag accacaaaga caatatagag cgttgccgcc
    19081 agcagatagt accaaccgaa ggttttagag acccagtcca gcgtgcgtcc aatccacagg
    19141 gccgagaagt cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag tccggcggaa
    19201 gtgtaaaaca ccaccggatt gattttgtcc ttttccctgc tgtgtgaaag gtctgtcatc
    19261 cagtatcccc actgttattg ttaccattta aaatcaaatt cgtaacaatt aagacacatt
    19321 ttatattgaa cgtccaatca ataaccgctt taatagataa acaacgctga tgaatggagt
    19381 ggcgaaaatg cccaaattgg ggatgcagtc gatccggcgc agacaactga tcgacgccac
    19441 gctggaagca ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg
    19501 tgcaggcgtt tctacgggga tcatcagcca ctatttcagg gacaaaaatg gtctgctgga
    19561 agcaaccatg cgcgatatca ccagtcagct gcgtgacgcg gttttgaatc gattacatgc
    19621 acttccgcag ggcagtgcag agcagcgatt acaggcgatt gttgacggga atttcgatga
    19681 aacgcaggtc agtaatgctg cgatgaaagc ctggttggcg ttttgggcca gcagtatgca
    19741 tcagccaatg ctttaccgtt tgcagcaggt gagcagccgc cgcttgttgt cgaatctggt
    19801 gagcgaattt caccgcgagt taccgcgcca acaggcacag gaagcgggct acggccttgc
    19861 cgcgctgatt gacggattat ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc
    19921 tctcgcacac tcgctgaccc gtcactttat tactcagcat ttacccaccg attaaccgag
    19981 gagacgtgat gtcccgaatg gcagaacagc agctttatat acatggtggt tatacctccg
    20041 ccaccagcgg tcgcaccttc gagaccatta acccggccaa cggtaacgtg ctggcgaccg
    20101 tgcaggccgc cgggcgtgag gatgtcgatc gcgccgtgaa gagtgcccaa caggggcaaa
    20161 aaatctgggc ggcgatgacc gctatggaac gctcgcgtat tctgcgtcgg gccgttgata
    20221 ttctgcgtga acgcaataac gaactcgcaa aactggaaac cctcgacacc ggaaaagcgt
    20281 attcggaaac ctcaaccgtc gatatcgtta ccggcgcgga cgtgctcgag tactacgccg
    20341 ggctgatccc gtcgctggaa ggcagccaga tcccattgcg tgagacgtca tttgtctata
    20401 cccgccgcga accgctgggc gtagtggcag ggattggcgc atggaactac ccgatccaga
    20461 ttgccctgtg gaaatccgcc ccggcgctgg cggcaggcaa tgcaatgatc ttcaaaccga
    20521 gcgaagtcac cccgcttacc gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc
    20581 cggacggtgt atttaacgtg ttgccgggcg tgggcgcgga gaccgggcaa tatctgaccg
    20641 atcatccggg cattgccaaa gtgtcattta ccggcggtgt cgccagcggc aaaaaagtga
    20701 tggctaactc ggcagcttcc tccctgaaag aagtgaccat ggaactgggc ggtaaatcgc
    20761 cgctgatcgt tttcgacgat gcagatctcg atctcgccgc tgatatcgcc atgatggcga
    20821 acttcttcag ctccggtcag gtgtgtacca acggcacccg cgtcttcgtt ccggcgaaat
    20881 gcaaagccgc atttgaacaa aagattctgg cgcgcgttga gcgcattcgc gcgggcgacg
    20941 ttttcgatcc gcaaactaac tttggcccgc tggtcagctt cccgcatcgc gataacgtgc
    21001 tgcgttacat cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac
    21061 tgaaaggcga tggcttcgat aacggcgcat gggttgcacc gaccgtgttc accgattgcc
    21121 gcgacgaaat gaccatcgtg cgcgaagaga tcttcggtcc ggtgatgtcg cttctcactt
    21181 atgaatctga ggacgaagtg attcgccgcg ccaatgatac cgactacggc ctagcggcgg
    21241 gcatcgtgac ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta
    21301 tttgctggat caacacctgg ggcgaatccc ctgctgagat gcccgttggc ggctacaaac
    21361 actccggcat tggtcgcgag aacggcgtga tgacgctcca gagttacacc caggtgaagt
    21421 ccatccaggt tgagatggct aaattccagt ccatattcta accaggaggt ttatttgcaa
    21481 tttgactaca tcattattgg tgccggctca gccggcaacg ttctcgctac ccgtctgact
    21541 gaagatccga acacaaccgt gctgctgctt gaagcaggcg gcccggacta tcgctttgac
    21601 ttccgcaccc agatgcccgc cgcgctggca ttcccgctac agggtaaacg ctataactgg
    21661 gcctatgaga cggaaccgga accgtttatg aacaaccgtc gcatggagtg cggacgcggt
    21721 aaggggcttg gcgggtcatc actgatcaac ggcatgtgtt acatccgtgg taacgcgctg
    21781 gatctcgata actgggcgca agaacccggt ctggagaact ggagttatct cgactgcctg
    21841 ccctactacc gcaaggccga gactcgcgat gtaggcgaga acgactacca cggtggcgat
    21901 ggcccggtga gcgtcactac ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt
    21961 gaagcgggcg tgcaggcagg ctacccgcgc acggacgatc tcaacggcta tcagcaggaa
    22021 ggttttggcc cgatggatcg caccgtcacg ccgcatggcc gtcgcgccag caccgcgcgt
    22081 ggctatctcg atcaggccaa atcgcgccct aacctgacca ttcgtactca cgccatgacc
    22141 gatcacatca tttttgacgg caaacgcgcg gtgggcgtcg agtggctgga aggcgacagc
    22201 accattccaa cccgcgcgac agcgaataaa gaggtgctgt tatgtgcagg tgcgatcgcc
    22261 tctccgcaga tcctgcaacg ttccggcgtg ggtagcgctg aactgctggc ggagtttgat
    22321 attccgctgg tgcatgattt acccggcgtc ggcgaaaatc ttcaggatca cctggagatg
    22381 tacctgcaat atgagtgcaa agaaccggtt tccctctacc ctgccctgca gtggtggaat
    22441 cagccgaaaa tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac
    22501 tttgaagcag gcggatttat tcgcagccgt gaggaatttg cgtggccgaa tattcagtac
    22561 catttcctgc cagtggcgat taactataac ggctcgaacg cagtgaaaga gcacggcttc
    22621 cagtgccacg tcggatcgat gcgctcgcca agccgtgggc atgtgcggat taaatcccgc
    22681 gacccgcacc agcatccagc gattctgttt aactacatgt cgcacgagca ggactggcag
    22741 gagttccgcg acgcaattcg catcacccgc gagatcatgc atcaaccggc gctggatcag
    22801 tatcgtggcc gcgaaatcag ccccggtgtc gaatgccaga cggatgagca gctcgatgag
    22861 ttcgtgcgta accacgccga aaccgccttc cacccgtgcg gtacctgcaa aatgggttat
    22921 gacgagatgg cagtggttga cggcgaaggc cgcgtacacg ggctggaagg cctgcgcgtg
    22981 gtggatgcgt caattatgcc gcagattatc accggcaatt tgaacgccac gactattatg
    23041 attggcgaga aaatggcgga tatgattcgc gggaaggatg cgttgccgag gagcacggcg
    23101 ggatattatg tggcaaatgg gaggccggtg agagcgaaaa aatgagtcgt gatgtgaact
    23161 gactcaggaa cctatcagat tgagagttac cgttctacta tgaggaccga atccttatat
    23221 aaaacactga ggtaactctc atgcttcata tccagtattc atgatgctgg ttttttgctg
    23281 atatttaacc cgaacactcc tgatacagcc tattaat
//
LOCUS       DAYPHN010000069        22365 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000069 DAYPHN010000000
VERSION     DAYPHN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.069"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..479
                     /locus_tag="ACG0BW_002529"
     CDS             <1..479
                     /locus_tag="ACG0BW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7259479.1"
                     /translation="CHFFNVDMIRVNFSKCLFHDCSMHGVKIKPWLPVKWTKELINDY
                     LYGCLLSLYSICARDIYNMIAGNNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYE
                     FARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            690..1013
                     /gene="ybjQ"
                     /locus_tag="ACG0BW_002530"
     CDS             690..1013
                     /gene="ybjQ"
                     /locus_tag="ACG0BW_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFK7259480.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            1010..1840
                     /gene="amiD"
                     /locus_tag="ACG0BW_002531"
     CDS             1010..1840
                     /gene="amiD"
                     /locus_tag="ACG0BW_002531"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFK7259481.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEEELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1837..2850)
                     /gene="ybjS"
                     /locus_tag="ACG0BW_002532"
     CDS             complement(1837..2850)
                     /gene="ybjS"
                     /locus_tag="ACG0BW_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFK7259482.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2949..4379)
                     /gene="ybjT"
                     /locus_tag="ACG0BW_002533"
     CDS             complement(2949..4379)
                     /gene="ybjT"
                     /locus_tag="ACG0BW_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFK7259483.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4390..5391)
                     /gene="ltaE"
                     /locus_tag="ACG0BW_002534"
     CDS             complement(4390..5391)
                     /gene="ltaE"
                     /locus_tag="ACG0BW_002534"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFK7259484.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRTQLAEVAAHWRAFLAR"
     gene            complement(5428..7146)
                     /gene="poxB"
                     /locus_tag="ACG0BW_002535"
     CDS             complement(5428..7146)
                     /gene="poxB"
                     /locus_tag="ACG0BW_002535"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFK7259485.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7279..8247)
                     /gene="hcr"
                     /locus_tag="ACG0BW_002536"
     CDS             complement(7279..8247)
                     /gene="hcr"
                     /locus_tag="ACG0BW_002536"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFK7259486.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNMRSPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNKVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8259..9911)
                     /gene="hcp"
                     /locus_tag="ACG0BW_002537"
                     /gene_synonym="priS"
     CDS             complement(8259..9911)
                     /gene="hcp"
                     /locus_tag="ACG0BW_002537"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFK7259487.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10055..10954)
                     /gene="lysO"
                     /locus_tag="ACG0BW_002538"
     CDS             complement(10055..10954)
                     /gene="lysO"
                     /locus_tag="ACG0BW_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFK7259488.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLTGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11438..12133)
                     /gene="aqpZ"
                     /locus_tag="ACG0BW_002539"
     CDS             complement(11438..12133)
                     /gene="aqpZ"
                     /locus_tag="ACG0BW_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFK7259489.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            12559..14217
                     /gene="ybjD"
                     /locus_tag="ACG0BW_002540"
     CDS             12559..14217
                     /gene="ybjD"
                     /locus_tag="ACG0BW_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFK7259490.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(14214..15170)
                     /gene="ybjX"
                     /locus_tag="ACG0BW_002541"
     CDS             complement(14214..15170)
                     /gene="ybjX"
                     /locus_tag="ACG0BW_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFK7259491.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15321..16436
                     /gene="macA"
                     /locus_tag="ACG0BW_002542"
     CDS             15321..16436
                     /gene="macA"
                     /locus_tag="ACG0BW_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFK7259492.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLATAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLDAGDEVVIGEAKPGAAQ"
     gene            16433..18379
                     /gene="macB"
                     /locus_tag="ACG0BW_002543"
     CDS             16433..18379
                     /gene="macB"
                     /locus_tag="ACG0BW_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFK7259493.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18451..18675)
                     /gene="cspD"
                     /locus_tag="ACG0BW_002544"
     CDS             complement(18451..18675)
                     /gene="cspD"
                     /locus_tag="ACG0BW_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFK7259494.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            18825..18899
                     /gene="yljB"
                     /locus_tag="ACG0BW_002545"
     CDS             18825..18899
                     /gene="yljB"
                     /locus_tag="ACG0BW_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFK7259495.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            18998..19318
                     /gene="clpS"
                     /locus_tag="ACG0BW_002546"
     CDS             18998..19318
                     /gene="clpS"
                     /locus_tag="ACG0BW_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFK7259496.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19349..21625
                     /gene="clpA"
                     /locus_tag="ACG0BW_002547"
     CDS             19349..21625
                     /gene="clpA"
                     /locus_tag="ACG0BW_002547"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFK7259497.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(21743..22201)
                     /locus_tag="ACG0BW_002548"
     CDS             complement(21743..22201)
                     /locus_tag="ACG0BW_002548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259498.1"
                     /translation="MSGLPAGRAKRVNPPLTVIFKEKRVRKYELFSHFICLFDSEPAE
                     EQQFVKYEFLSSLYGSEPSTSTSFAEYSFLSCLYGSERVRKGFTAPRRFLSCLYGSER
                     VRKGFTAPRRFLSCLYGSELKYYTFSSLLIKQNHLIFLLAPFFILTFQHH"
BASE COUNT         5477 a         5599 c         6053 g         5236 t
ORIGIN      
        1 attgccattt ttttaatgtt gatatgataa gagttaattt cagcaaatgt ctttttcatg
       61 actgtagtat gcatggtgtc aagataaaac catggttgcc agtaaaatgg acaaaagaat
      121 taataaatga ttatttgtat ggctgtttac tatctctgta ctctatctgt gcaagagata
      181 tatataatat gatcgccggt aataacgtga aggttgctgc ggatgctttc cttgaaatac
      241 tttattctct taaaaataaa tattgtatta aactattaag cgctcaggac cgggcgttta
      301 tatacgaatt tgcaagaatg atcttcgctt atattaatga caaaagcatt gagatcttat
      361 tactttcttg ttttgcggcg gctgaccaaa aagcaatcca gagatatata cctcaggcgc
      421 aagatgggga agactttagg aatcatttac aatataagtt gactcttccc actcattaat
      481 gttgctggta ctacataata cgacaggtaa gcggtgctcc ataataaaac taatatcggg
      541 ttatgcaacg gataattgta caggcttatg tacctgtttt tgacacaatg agatgatttc
      601 ctaaactgat caaaaccttc ttcccaacat ggcatatcag tccgataatg ctttagagat
      661 cgaaggctta acaaacaagg agatcgatca tgcaattttc tacaacccca actctggaag
      721 gccagaccat cgtcgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata
      781 ttttccgtga tttctttgcc ggtatccgcg atatcgttgg tggacgctcc ggtgcctacg
      841 aaaaagagct acgtaaagcg cgggagatcg cctttgagga actaggctcc caggcgcggg
      901 cactgggggc cgatgccgtg gttggtattg atatcgacta cgaaacggtc gggcaaaacg
      961 gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt
     1021 cttttggctg gtcgccgccg ctctgttact ggcagggtgt gcaggcgaaa aaggcattgt
     1081 cgagaaagag ggatatcagc ttgatacccg acgccaggcg caggcggcgt atcctcgcat
     1141 taaagtgctg gtgatccact acaccgcaga tgattttgat agctcgctgg cgacactgac
     1201 cgataagcag gtcagctcgc attatctggt ccctgcggta ccaccgcgat acaacggtaa
     1261 accgcgcatc tggcaactgg tgccggaaga agaactggcc tggcatgcgg ggattagcgc
     1321 ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc attgagctgg aaaaccgtgg
     1381 ctggcagaaa tcagccggag tgaaatattt tgccccgttt gaacaggcac agattcaggc
     1441 gcttattccg ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt
     1501 ggcacatgcg gatattgcgc cgcagcgcaa agacgatccg gggccgttat tcccctggca
     1561 acaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtga acttttacct
     1621 tgccgggcgc gcgccgcaca caccggtaga taccgcgtca ttgctggagc ttttggcgcg
     1681 ctacggttat gacgttaaac ctgatatgac accgcgcgag cagcggcgcg tgattatggc
     1741 gttccagatg catttccgcc cgacgttata caacggcgaa gcggatgcag aaactcaggc
     1801 gattgccgaa gcgttgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc
     1861 gcgtagccag gcggcagttt tctcgatacc ttcatccagg gtgaggactg gttgataacc
     1921 taactcttcc tgcgcacgcg taatatccag cgtaaagtca aaattcaact tggagacgcc
     1981 gtagtgggtc agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc
     2041 gatcatatcc agcatcgggt aggggacgga acgaatacga cagtcaatat tcaactcgtc
     2101 gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg ccattggtga tgttgtacac
     2161 acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc agccacatgg cgtgcacggc
     2221 attttcatag taggtcatat ccaccagcgc actgccgccg tgcggtaaca gaatgctgcc
     2281 atagtggtgc atcatatgcg ccagacgggg aataaagact ttatcgtgcg gtccgaacag
     2341 actttgtggg cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat
     2401 cacctcttcg ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa
     2461 atcttcttta atatcgcgat ggtggtgata atcgaagtac agggaggggg aagagatatg
     2521 aataaagtta cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac
     2581 gttagccaga tcgaaagcct gttgtgttcc ccagggcgag gtaaagctgg agcagtgcca
     2641 cagcgtatca atgcccgcga gcatcacttt agcttgtgat gagaccagct cggtcagatc
     2701 cgccggaaca aactctgcgc ccattttttc cagcaattta cccattgcct cgttgcgacc
     2761 ggtcgctcgc acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc
     2821 gctggtggcg ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaatgacgt
     2881 gcgcattctt ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt
     2941 tttgtcgatt aatctgtgct ttgttctgcc agtctggcga tttgttttgc cattccgcga
     3001 aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc
     3061 ggatgccaga atgcgcgaac atcgatagta cgatagtcgc ctttatcttc cagggtaaaa
     3121 cacagtcgtc ccagccccgg cgctttcatg ccaaataaca gcgtaagttg tttttccggt
     3181 tcaacgacaa tcactttcca gctatccacc gcatcgccgg tctgcaaata ttcacgctcc
     3241 gggcggcctt tcgccagctt atgaccgatc gcgcggtcca tcaacgcccg ggtttgccac
     3301 aaaatattgc caaagaaata acgctcttta ccgccgattt gattcaccac ctgccataaa
     3361 gccgcaaggc tggcggacgt tttaacggta aaccccgcct gtttggcgaa ataaccgtac
     3421 tccggtcgcc agcgggcaaa ggcctgagcg tcgtatcccc agtcgctgga gttgaccagc
     3481 ttttcttcct ctttcagggt gcgacgtacc gcgtcatcga aagcgatcag ccgttgtggg
     3541 atgagtgcac gtagcgcggt gtcatccgcc agcagatcgt gtttcagccc ctgaatcaac
     3601 gccctggcgg tggtgggcgg tacggaagta atcacattga gaaaccacac cgaaatccag
     3661 cgggtcggga gggggatggg gatcaaccag cggcgcttac cgctcaccgc cataaaatgt
     3721 tcaaactgtt gctgataact gagcacttct ggtccggcgg cttcgaagat gcgatgttcg
     3781 ctggccggat gatctaacag cgccaccaga tagtgcagca agttttccag cgcgatgggc
     3841 gtggtgcgtg aacgaaccca gcgtggcggc gttaacactg gcaggttgta gaccatatcg
     3901 cgcatgactt cgaacgccgc tgaacctgca ccaacgatta ttccggcacg caattcggtc
     3961 accgggacac ccgcttcacg aagaatatcc gccgtagcct gacgggcacg cagatggtcc
     4021 gactgctcat gtggcggggc ctgcaacgaa ctgagaaaga ttaattgctt aactgggact
     4081 tcacgtagcg catcgcggac gttgagagcc acctggcgct cctgagcgat aaaatcgccg
     4141 ccttcgccca tgctgtgcac cagaaaatag accgtatcga tatcctgcaa cagggccgga
     4201 aggttatccg gccagctgag atcgacttta tggcaactga catttgccag ttgcagcttt
     4261 gcaagcctgt cgacatgacg tgccgccgcc aggatctgat gcccctgctg gctgagtgtt
     4321 cgcaccagat gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg
     4381 tctccctcct taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg ccagctgagt
     4441 gcgcgagacg tcaagatggg tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt
     4501 tctcgctttc atgtattcgc ctaacgcggc agcattttct tccccgacgc gaacaaacag
     4561 catattggtg tcctgacgca tcacatccgc gcctgcttca cgcaactgct ccgccatcca
     4621 ggcggcgttg tcgtggtctt cctgtaagcg cgcgacgtta tttttcaggg catacatccc
     4681 ggcggcagcc agaatgccgg actggcgcat tccgccaccg gtcattttcc gccagcggat
     4741 ggcacgttta atgtaatcac gattaccgac cagtaacgag ccgactggcg tcccaagtcc
     4801 tttcgacagg caaatggtga acgaatcaca atattgcgtg atctctttca gttcgcagcc
     4861 gtaagccacc acggcattaa agatgcgcgc accgtcaaca tgcagcgcca gattgcgctc
     4921 gcgagtaaat tcccatgctt ctttcaggta ttctcgcggc aacactttgc cgttgtgagt
     4981 gttttccaga ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt tgattttcat
     5041 cgccacttta tccagcggta gcgtgccgtc ggcagccgcg tcgatgggtt gcggttgaat
     5101 actgcccagc accgccgcgc caccggcttc aaacagatag ttatgcgcgg cctgaccgac
     5161 aatatactct tcgccgcgtt cacagtgact gagcagagcg accaggttgg cctgagtgcc
     5221 ggtaggcaga aaaatggcgg cttctttacc ggaaagctct gctgcatagt cctgcagagc
     5281 attaacggta gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca tcatcgcttc
     5341 gagcatggcg cggctcggtc gggtaacggt atcactgcgt aaatcaatca tggcatgtcc
     5401 ttattatgac gggaaatgcc acccttttta ccttagccag tttgttttcg ccagttcgat
     5461 cacttcatca ccgcgtccgc tgatgattgc gcgcaacata tacaggctaa aacctttggc
     5521 ctgttcgagt ttgatctgcg gtggaatggc taattcttct ttggcgacca ccacatccac
     5581 cagcaccgga ccgtcgatgg aaaaggcgcg ttgcagggct tcatcgactt cagaggcttt
     5641 ttccacgcga atacccgtaa ttccgcacgc ttcggcaatg cgggcaaagt tggtgtcatg
     5701 tagttcggtg ccgtcagtca aatagccacc agctttcatc tccatcgcca caaagcccag
     5761 cacgctgtta ttaaagacga taattttcac cggcagtttc atctgcacta ctgagaggaa
     5821 atcgcccatc aacatgctaa aaccgccatc gccgcacatg gcgaccacct gacgttccgg
     5881 ctcggttgcc tgagcaccca gcgcctgcgg catggcgtta gccatcgaac cgtggttaaa
     5941 cgaacctaac agacgacgct tgccgttcat tttcagataa cgtgccgccc acaccgttgg
     6001 cgtaccaacg tcacaggtaa agatggcgtc atcggcggca aaatgactaa tttgctgcgc
     6061 cagatattgc gggtgaatgg ctttctcgct cggtttagct aaatcgtcca gccctttgcg
     6121 ggcgtcgcgg taatcttcca gcgctttatc cagaaacttg cgatcggctt tttcttccac
     6181 cagtggaagc aatgcacgca gagtcgactt gatatcgccg accagtgcca tatccacctt
     6241 gctgtgagcg ccgatgctgg ctgggttgat atcaatctga atgattttgg catcggtcgg
     6301 gtagaaggcg cggtagggga actgagtacc gagtagcact aacgtgtcgg cgttcatcat
     6361 ggtatggaaa cctgacgaga agccaattaa tcccgtcatt ccgacatcat acggattatc
     6421 gtattcgaca tgttctttac cgcgcagggc atgaacaata ggcgctttaa ttttcccggc
     6481 aaactcaact aactctttat gcgcccccgc gcagccgctg ccacacatca gggcgatatt
     6541 gctggaataa cgcagcagtt gcgccagttt gcgtaactct tcttcttccg gcgtcacgac
     6601 tggttgtggc gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac
     6661 gtcgcccggt aatacaacaa cggaaacgcc acggttaagc accgctttgc gcatggcaat
     6721 cgccagcact tgtgggatct gctccgggct ggaaaccagc tcgcaatagt gactacattc
     6781 gcggaatagc tcttgtgggt gggtttcctg gaaatagccg ctgccaattt cgctggaggg
     6841 aatatgagcg gcaatcgcca gtaccggaac gtgattgcgg tggcaatcaa acaggccgtt
     6901 gattaagtgc aggtttccgg ggccgcacga tccggcacag accgccagtt ctcctgaaag
     6961 ttgtgcttca gcgccagcgg caaaggccgc cacttcttca tggcgggtgg acatccactc
     7021 gatggtgccc atgcgattaa gactgtcact aagaccgttc agagagtcgc ccgtgactcc
     7081 ccagatgcgt ttcacgcctg ccgattcgag tgttttggcg atataagctg caaccgtttg
     7141 tttcatggtt ctccatctcc tgaatgtgat aacggtaaca agtttagttc atctgacgga
     7201 gggggaaggg atgggggaaa aaggaggcac taacggttaa atagcccgat gaaaggaata
     7261 tcatcgggca taaggcgatt atgcgagaac caaatccccc tgcggatggc aggagcaggc
     7321 cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc attgtgctgc tcaccgtata
     7381 ttcaccggaa accactttcg tcttacagca gccgcaaaca cccgcacggc aggcagcgac
     7441 aaccggaact ttattgcttt ccagcgcctc cagtagcgtg gtgccaaccg gggcgtaaaa
     7501 ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt ccgctactgg
     7561 ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc gctttcactt cctgctctac
     7621 ccaatccata tacggagccg ggccgcaggt catcacggta cgtgaagcta agtcagggac
     7681 acctgccagc agttcgcgag tgagacgacc agcgataaag ccttcggtaa cgttattttc
     7741 tgccaccagc gtcaccggat agttgcgcca ctcatcggcg aaaataacat cctgcggcga
     7801 acgcatgttg tagatcacct gcacatcggc ctgcggacgg ttcttcgcca gccagcgacg
     7861 catcgacata atcggcgtga cgccgcagcc tgccgccagc aacaggaatt tatcttctgc
     7921 tttatcgtcg caggtaaatt cccccattgc gtctgaaagc cagagataat caccgcgttt
     7981 tacatcgcgc gtcagccact gggagccgac tccgtcatca atccgccgca cagtcagggt
     8041 gatgtattca ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg tttccgctga
     8101 gttacgcacg ctgaccagtg catattgccc ggcgcgatat gggtagtaat cgtggcaaat
     8161 cagggaaatc gtccacacat ccggcgtttc ttgcgtaatg tgatgaacct gcatccgcca
     8221 cgggcattga ttcgttggca tcgtcatcga caaactcctt acgcgctcaa cagttgcttc
     8281 atgtcttctt caacagtggt gatagaacgc aggccgaatt tctcgttcag caccgccagc
     8341 aggtctggtg tcaggaaacc aggtgcagtc gggccggtga cgatattttt cacacccaga
     8401 gaaagcagtg tcagcagaat gacaatcgct ttctgttcaa accaggagag caccagtgac
     8461 agcggcagat cgttgacacc gcagcccagt ttttctgcca gcgttaccgc cagaataatt
     8521 gctgagtaag catcgttaca ctgaccagca tccaccagac gcggcagacc ttcgatatcg
     8581 ccaaactcga gtttgttaaa gcgatattta ccacaggcga gagtgaggat caggcagtca
     8641 tccggcacgc tggtggcgaa atcggtgaag tagtgacgct cgccgcgtgc gccgtcacag
     8701 ccgccaagca ggaagatatg acgcagtttt tcacggctta ccagatcaat cagcgtatcc
     8761 gctgcgccaa gcagcgtttg acgaccaaag cccacggtga tcaggtgcgg aatttcgctg
     8821 tacgggaagc ctgccatttg ttgcgcctgg gcaatgaccg cagagaaatc ttcgccatcc
     8881 agatgacgca cacccggcca gccaacgatg ctgcgggtcc agatacgatc gtcataagcg
     8941 cctacggttg ggtcgatgat gcagttcgag gtcatcacga tggggccagg gaaacgagcg
     9001 aactccactt gctgattctg ccagccgctg ccgtagttac cgaccagatg cttgaattta
     9061 cgcagctccg ggtagccatg cgcaggcagc atttcgccgt gggtgtagac attaacgccc
     9121 gtgccttcgg tctgttccag caggttgtag agatctttga gatcgtgacc ggaaatcaga
     9181 atgcatttac ccgccgtcgc tttgacgttg acctgggttg gggtcgggtg accgtattta
     9241 ccggtttcgc ctgcgtccag aatgctcatc actttgaagt tcatctggcc gatttccatt
     9301 gaacactcaa gaagcgcgtt catatcggca ggccaggttc ccagccacgc catgatttta
     9361 tggtactggg cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag
     9421 gccgccgcac ctttcaggcc atacaggcac agcaggcgca ggccgagaat gttttcgcca
     9481 atcgccgctt tatctttgtt aggggtaaat tctgctgcct gacgttgcag ctcgccaaga
     9541 tcatcgctca ccagttgcag gtcagccatc gggttatcga cgcgggcgtt ggcatctaca
     9601 gccaggcatt gtgctttcag cgcctcgcgc agggcaatcg cttcacgagc gtagccgact
     9661 atacgcggag aatcgaagtt aacgttggtc agggttgaga aaaatgcgcg tggcgcaaag
     9721 ctgtctacat cgtggttgat gatgccgtat tcacgcgctt ttaccgccca ggccgaaagc
     9781 ccttgcagcg ccgcgatgag taaatcctga aggtcagagg tttccgccgt tttaccacac
     9841 atcccctgcg cgtatgagca gccgtttcct gccggagtac ggatagtttg ttcacattgc
     9901 acacaaaaca tgatcacacc ttttaaagtt atatttaata tacatgttta aggttaagac
     9961 gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg atttagcgca
    10021 attttggcgg cagggatcta ccgccagaga ggtattacgc ggaaaaaaag gcgatgagga
    10081 tcggcactaa cagactaaga ataaaaccgt gaacaatcgc cgccgggacc atatccagcc
    10141 cgccagtacg ttgaagaacg ggcagggtga aatccattga tgtggcaccg cataagccca
    10201 gtgcggtaga gcggctgcgg cgaatcagcc cagggatcaa cataattgca atcagctcac
    10261 gggccagatc attaaaaaac gccgcgctcc cgattaccgg accaaaagat tcagtcaata
    10321 aaataccgga aagagaatac cagccgaaac cagaggccat tgccagcgcg gtattgatgg
    10381 gaagatcaag aataaaggcg ttaattaaac caccagttaa tgaactggca accaccacca
    10441 cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg ccattattgc
    10501 gcaactgaat accgacgagg aaaagtagca gaattaaggt gtattcactg gcttcggtcg
    10561 catgttgtaa gaaagccagt ccactcaggc caatggcaaa accaatcact actacgccgc
    10621 acagttttag cgactccagc gccatcgcaa tacgcgacgg gagtttttct tgctgatggt
    10681 gattgcgcca tggcaggcct cgctccagcc acataagggc ggcaatatta cacagtaaaa
    10741 taacggtaat actgacggcg gaataatgca gaatcgccaa caggttactg gcgaggttat
    10801 cgagaaacgc cagactgata cccataaaaa agagaataag gtaaaccatc cagcttaata
    10861 gctgattaat aactcttaat gcagcttttt ggcgaagagg aatgaggtaa cccacaatca
    10921 ggggaaccag aatgattaac agcccagaaa acatgaaaac ccagtccttg caaagatgaa
    10981 gtcgaaatgc gcgatgacac actactgaaa gcggaaggac gagtaaagtt gcaattaaaa
    11041 ggaaatgtta tgcataagga tcggtagggt attcattttc atttaaagat attcttgcgc
    11101 tttaattaca aaccgcaccg gtgttggtgg cgtcaaaatc gccgaggcgt tccctgaagg
    11161 ccggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc
    11221 ccgacccggt agccgcaagg gataagtcga gggcaccgcg cagcggcgat tttgttcgcc
    11281 agagcccggg ggtgcagggg gcggcggcga ttggccgccc cctgcgcgct ccctgcgcca
    11341 gtgactatat gtggcttaac acatcaagga gcgcaacagg aagatgaatc aatatattta
    11401 aaaaaggcct gacattgcgc caggccttct gcgttaatta attacgtttt tccagcaggg
    11461 tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag aagaaccaca
    11521 gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc gccgggttaa
    11581 cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatag
    11641 cgatcggcgc aaaaccagca ggagcgaatt tgtcggttgc gccgtggatc accagcagga
    11701 aacctgcact caataccagt tcaactacca gtgcggaaag catggaataa ccgcctggcg
    11761 aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac
    11821 cactggcaat taaatacagc agcgccgccg caacaatacc gccgacaacc tgggcaatta
    11881 cgtagccaac gacttctttt gccgggaaac gtccgccagc ccataaacca atagtgaccg
    11941 ccgggttaaa atgaccacca gaaatatgac caactgcaaa ggccatcgtc agaacggtca
    12001 gaccgaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag cctgcggcca
    12061 gtacggcact accacagcca ccaaaaacaa gccagaaagt accaaaacat tcagctgcta
    12121 attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta
    12181 tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg gctattttaa aaacgaaggc
    12241 gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag cgggttgccc
    12301 catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat cctttaggcg
    12361 taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgca ggcttatcag
    12421 caagagggag tataacgcga ttattcgctc atttttcaga catttgccat gcttaaatgt
    12481 gatgtcatca cgtattagca tggcctttcc cgttatactg ccagcgtaaa ggataagtca
    12541 catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt tcgcggtatc
    12601 aaccgtttgt cgttgatgct ggaacaaaac aacgtcctga ttggggagaa cgcgtggggt
    12661 aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga tctctaccat
    12721 tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga acatcatctg
    12781 catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg ccgctatcgg
    12841 ccgctggaag cgtgctggac gccatgcact gatggctatc accgtatttt ttatcgtctg
    12901 gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct cgataaagac
    12961 ggacatccga ttgatgtcga ggatattaac gaccaggcac gccatctggt gcgtttaatg
    13021 ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg cacggtgcca
    13081 aatgtcccta atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg tgagttatcc
    13141 tcacatccgc aaaatctctc tgatgggcag attcgtcagg gactttccgc aatggtacag
    13201 ctgcttgagc attatttctc tgagcagggg gccggacagg cgcgatatcg tttaatgcgg
    13261 cggcgagcca gcaatgagca acgaagctgg cgctatctgg atatcatcaa ccggatgatt
    13321 gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc tactttgttg
    13381 caggcaaaag gcacattgcg actggataaa gacgcccgtc cattgttgct gatcgaagat
    13441 ccagaaaccc gtttacaccc cattatgctt tcagttgcct ggcatctgtt gaatcttctg
    13501 ccattgcagc gcattgccac caccaactcg ggtgagttgc tttcgttaac gccggtagag
    13561 catgtttgcc gactggtacg tgagtcctcg cgcgttgccg cctggcgtct ggggccgagt
    13621 ggtttgagta ccgaagatag ccgacgcatc tctttccaca ttcgttttaa tcgtccatca
    13681 tcgctgtttg cacgctgctg gctgctggtg gaaggggaaa cggaaacctg ggttatcaat
    13741 gaactggcgc gtcagtgcgg acatcatttt gatgctgaag ggatcaaggt cattgagttt
    13801 gcccagtccg ggctaaagcc actggttaaa tttgcccggc gaatggggat tgaatggcat
    13861 gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg cagcctgttg
    13921 aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct ggatatggaa
    13981 cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca aatcccggaa
    14041 aatgtaccga tgaatctgcg caaaattatc tcgaaagcga tccatcgctc ttccaaaccc
    14101 gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggattc cgtaccgacg
    14161 ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc ggattaaccg
    14221 cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg ccgacggtat
    14281 tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg tgctggaagg
    14341 cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc gttgtaatca
    14401 gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg tgtttcattg
    14461 ctgacggcaa tgatctgctc tacctgcaaa cgttgggcaa acagacaggc cgcttccatc
    14521 acaaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat ttcctgatgt
    14581 ggaatttccc attttgcgcc ttgcagtccg ccaataaaca ttgttctttt cccctgatat
    14641 tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg gaacaggatt
    14701 gtactgtcac cttctttatc cattgagatc atcatggtca gctcaagtgt gaactgctcg
    14761 ccgtttttgc cttccagctt cgccagttgc agcccggggg tattcaaata taagctgaat
    14821 tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc ctccagcaat
    14881 tgcttacggc taagattcgc cgcaaggtaa gggcgatgca gacgcacagg cagtcgcggc
    14941 tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat ccactcaacg
    15001 cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact acggcgatgc
    15061 cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga caggctgctg
    15121 agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat gaaatcaatg
    15181 gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat agtgtgatta
    15241 ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc ggattggcat
    15301 atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt tacgttattg
    15361 cgctggtgat agtcattgcc gggctgatta cgttatggag aattcttaac gcacccgtgc
    15421 cgacttatca gacgctgatt gtgcgccccg gtgatttaca gcaaagcgtg ctggcgaccg
    15481 gaaagctgga cgcgctgcgt aaggttgatg tgggcgcgca ggtcagcggt cagttgaaaa
    15541 cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg gttattgatc
    15601 ctgaacaggc tgaaaaccag attaaggagg tggaagcaac gctgatggag ctacgcgcgc
    15661 agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc cgtcagcaac
    15721 gtcttgcaca aacgcaagcg gtttcacagc aggatctcga taccgccgcg acggagatgg
    15781 ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat caggcctcac
    15841 tcgctacggc taaaaccaat ctcgattaca cccgcatcgt tgctccgatg gccggggaag
    15901 tcacgcaaat caccactctg caaggccaga cggtgattgc cgcacaacaa gcaccgaaca
    15961 ttctgacgct ggcagatatg agcaccatgc tggtaaaagc gcaggtttct gaagcggatg
    16021 taatccacct gaagccgggg caaaaagcct ggtttacggt acttggcgat ccactgacgc
    16081 gctacgaggg gcaaatcaag gatgtactac cgacgccgga aaaggttaac gacgctattt
    16141 tctattacgc ccgttttgaa gtccccaacc ccaatggttt gctgcggctg gatatgaccg
    16201 cgcaagtgca tattcagctc accgatgtga aaaatgtgct gacgatccct ctgtcggcgt
    16261 taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat ggtgaaacac
    16321 gcgaacgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt gtcaaagggc
    16381 tggacgcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca caatgacgcc
    16441 tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc aggttgaggt
    16501 gctgaagggc atcaccctcg atatttatgc tggtgagatg gtggcgattg tcggcgcttc
    16561 gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg ccaccagcgg
    16621 cacctatcgc gtagccggtc aggatgttgc cacgctggac gccgatgcgc tggcgcaact
    16681 gcgtcgcgaa cacttcggct ttattttcca gcgttaccat ttgctttcgc atttaaccgc
    16741 cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac agcggctgct
    16801 tcgcgcccag gagttgctgc aacgactggg gctggaagac cgtacagagt attatccggc
    16861 acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga tgaacggcgg
    16921 tcaggtaatt cttgccgatg aaccaaccgg cgcactggac agccattctg gcgaagaggt
    16981 gatggcgatc ctgcatcagc tgcgtgatcg cgggcatacg gtgattatcg ttacccacga
    17041 tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg aaattgtgcg
    17101 caatcctccc gccgttgaaa aagtgaatgt tgctggcggg acggaacctg ttgtcaacac
    17161 ggcctctggc tggcggcagt ttgtcagtgg ttttaacgag gcgctgacga tggcatggag
    17221 ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta ttatcggtat
    17281 tgcgtcggtg gtttcaattg tcgtggtggg cgacgccgcc aaacaaatgg tgctggcgga
    17341 tattcgttct attggtacga atactattga tgtctatccc gggaaagatt ttggcgatga
    17401 cgatccgcaa tatcagcagg cgctgaagta cgatgactta atcgccatcc aaaaacaacc
    17461 gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt ataacaatgt
    17521 tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct atggcatgac
    17581 cttcagtgaa gggaacacct ttaatcagga gcagctgaac ggtcgtgcgc aggtggtggt
    17641 gctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg ttggcgaggt
    17701 gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa aacagtcgat
    17761 gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt ccgggcgagt
    17821 tatgggccag tcgtggctga actccattac tgtcagggtg aaagaaggat ttgacagcgc
    17881 cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga aggatttctt
    17941 tacctggaac atggacggcg tcttgaaaac cgttgaaaag accacacgta ctttacaact
    18001 gtttcttacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg taatgaatat
    18061 tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg ctgtaggtgc
    18121 gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtgctggttt gcctggtcgg
    18181 tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc ttttcttacc
    18241 cggctgggag attggttttt cacctttggc gctgctgctg gcgtttctct gctcgacggt
    18301 caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg atccagtaga
    18361 tgctctggca cgagagtaat tttgagataa aaatgccagc cgatcgggct ggcattttgc
    18421 ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg
    18481 cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg
    18541 tccagctttt agcgttctgt aaccatccat ctgaatggtg gaataatgag cgaaaatatc
    18601 ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac
    18661 agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa
    18721 ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa
    18781 ttagggcgag ccgtcaagca tttgacaggg gccagggggc aggtatgaat caaaaatttg
    18841 aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat
    18901 agttatctat catgtggagt agattggtca ggcaaataag ctcttgtcag cggcagggcg
    18961 ttctgccgat aaccgtaacc gaagatgata actgacaatg ggtaaaacga acgactggct
    19021 ggactttgat caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta
    19081 taaagtgata ttagtcaatg atgattacac tccgatggag tttgttattg acgtgttaca
    19141 aaaattcttt tcttatgatg tagaacgtgc aacgcaattg atgctcgctg ttcactacca
    19201 ggggaaggcc atttgcggag tctttaccgc cgaggttgca gaaaccaaag tggcgatggt
    19261 gaacaagtac gcgagggaga atgagcatcc attgctgtgt acgctagaaa aagcctgaat
    19321 gcaggcataa aaattggggg aggtgcctat gctcaatcaa gaactggaac tcagtttaaa
    19381 tatggctttc gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt
    19441 actggcgctg ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt
    19501 ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa accacacccg ttctgcctgc
    19561 cagtgaagag gagcgcgaca cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc
    19621 ggtcttccat gtccagtcct ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc
    19681 tatttttagc gaacaggagt cgcaggcggc atatctgttg cgtaaacatg aagtcagccg
    19741 tctcgatgtg gtgaacttta tctctcatgg cacgcgtaaa gacgagccga cacagtcttc
    19801 tgatcctggc agccagccaa acagcgaaga acaagctggt ggggaggaac gtatggagaa
    19861 tttcacgacg aacctgaatc agcttgcgcg cgtgggagga atcgacccac tgattggtcg
    19921 tgagaaggag ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa acaacccgct
    19981 gctggtgggg gaatctggtg tcggtaaaac cgcgattgcg gaaggtcttg cctggcgaat
    20041 tgttcagggc gatgtgccgg aagtgatggc tgactgtacg atttactctc tcgatatcgg
    20101 ttctctgtta gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta aagcgttgct
    20161 caagcagctg gagcaggata ctaacagcat cctgtttatt gatgagatcc acaccattat
    20221 cggtgcgggt gcagcctctg gtggccaggt cgatgcggct aacctgatca aaccgctgct
    20281 ctccagcggt aaaattcgcg taattggttc gacaacctat caggagttca gcaacatttt
    20341 cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat
    20401 cgaagaaact gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc accacgacgt
    20461 gcgttatacc gcaaaagcgg tgcgtgcagc ggtagagctg gcggtgaaat acattaacga
    20521 tcgtcatctg ccggataaag ccattgacgt tatcgacgaa gcgggcgctc gcgcacgcct
    20581 gatgccggta agcaaacgca agaaaaccgt taatgtggcg gatattgagt ccgtggtggc
    20641 ccgtattgcg cgcattccag agaagagtgt ttcgcagagt gaccgcgata ccctgaaaaa
    20701 cctcggcgat cgcctgaaaa tgctggtctt cggtcaggat aaagccattg aggcgctgac
    20761 tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt
    20821 cctgtttgca ggccctaccg gggtcgggaa aacagaggtg acggtacagc tttcgaaagc
    20881 tttgggcatt gagcttctgc gctttgatat gtccgagtat atggaacgcc ataccgtcag
    20941 ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat cagggcggtt tgctgactga
    21001 tgcggtcatc aagcatccac atgcggtgct gctgctggac gaaatcgaga aagcgcaccc
    21061 ggacgtgttc aatattctgt tgcaggtgat ggacaacggt acgctgaccg ataacaacgg
    21121 acgcaaagcg gacttccgta acgtggtatt ggtgatgacc accaacgctg gggtacgaga
    21181 aactgagcgt aaatcgattg gtcttatcca ccaggacaac agtaccgatg cgatggaaga
    21241 gatcaagaag atctttacgc cggagtttcg taaccgtctc gacaacatta tctggttcga
    21301 tcatctctcc accgacgtga tccatcaggt agtggataaa ttcatcgtcg agttgcaggt
    21361 tcagttggat cagaaaggtg tttctctgga agtgagccag gaagcgcgta actggctggc
    21421 cgagaaaggt tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa
    21481 cctgaaaaaa ccgctcgcca acgaactgtt gtttggttcg ctggtggacg gcggtcaggt
    21541 gacggttgcg ctggataaag agaaaaatga gctgacttat ggattccaga gtgcacaaaa
    21601 gcacaaggcg gaagcagcgc attaatctga ttgtcaggta ggttggtgaa gtccgtaatc
    21661 tcgaaagagg ttacggactt tttgtttgta ggctggggcg gtgaaaaccc tatttttgga
    21721 ggtgaaggta agttgttgat aattaatggt gctggaaggt aagaataaaa aagggtgcca
    21781 gcaggaaaat gagatgattt tgctttatta acaacgagct aaacgtgtag tatttgagtt
    21841 cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt
    21901 cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt
    21961 cactgccgta caggcagctt agaaagctgt attcggcaaa tgaagtggat gttgatggtt
    22021 cactgccgta caggctgctt agaaattcgt acttaacgaa ctgttgctcc tctgctggtt
    22081 cactgtcgaa caggcagata aaatgcgaaa aaagctcgta ctttcgtacg cgcttttctt
    22141 taaatatgac ggtgaggggg ggattgactc gcttcgctcg ccctgcgggc agcccactca
    22201 ctgtgttcgt ggtctgtcca actggctgcg ccagttgtcg aaccccggtc ggggcttctc
    22261 atcccccctg gagtgcaata tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
    22321 gaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccg
//
LOCUS       DAYPHN010000070        22346 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000070 DAYPHN010000000
VERSION     DAYPHN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.070"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            71..1681
                     /gene="yeaV"
                     /locus_tag="ACG0BW_002549"
     CDS             71..1681
                     /gene="yeaV"
                     /locus_tag="ACG0BW_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFK7259499.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINSVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            1713..2837
                     /gene="yeaW"
                     /locus_tag="ACG0BW_002550"
     CDS             1713..2837
                     /gene="yeaW"
                     /locus_tag="ACG0BW_002550"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFK7259500.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKESVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            2893..3858
                     /gene="yeaX"
                     /locus_tag="ACG0BW_002551"
     CDS             2893..3858
                     /gene="yeaX"
                     /locus_tag="ACG0BW_002551"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFK7259501.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3912..5027)
                     /gene="rnd"
                     /locus_tag="ACG0BW_002552"
     CDS             complement(3912..5027)
                     /gene="rnd"
                     /locus_tag="ACG0BW_002552"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFK7259502.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5109..6794)
                     /gene="fadD"
                     /locus_tag="ACG0BW_002553"
     CDS             complement(5109..6794)
                     /gene="fadD"
                     /locus_tag="ACG0BW_002553"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFK7259503.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(6999..7580)
                     /gene="yeaY"
                     /locus_tag="ACG0BW_002554"
     CDS             complement(6999..7580)
                     /gene="yeaY"
                     /locus_tag="ACG0BW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFK7259504.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7619..8314)
                     /gene="tsaB"
                     /locus_tag="ACG0BW_002555"
     CDS             complement(7619..8314)
                     /gene="tsaB"
                     /locus_tag="ACG0BW_002555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFK7259505.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8372..10282)
                     /gene="yoaA"
                     /locus_tag="ACG0BW_002556"
     CDS             complement(8372..10282)
                     /gene="yoaA"
                     /locus_tag="ACG0BW_002556"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFK7259506.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKECFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCTDRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10414..10758
                     /gene="yoaB"
                     /locus_tag="ACG0BW_002557"
     CDS             10414..10758
                     /gene="yoaB"
                     /locus_tag="ACG0BW_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFK7259507.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11180..11479
                     /gene="yoaC"
                     /locus_tag="ACG0BW_002558"
     CDS             11180..11479
                     /gene="yoaC"
                     /locus_tag="ACG0BW_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFK7259508.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIKNPEYFSTYMQEELKALV"
     gene            complement(11599..11778)
                     /gene="yoaH"
                     /locus_tag="ACG0BW_002559"
     CDS             complement(11599..11778)
                     /gene="yoaH"
                     /locus_tag="ACG0BW_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFK7259509.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            11852..13213
                     /gene="pabB"
                     /locus_tag="ACG0BW_002560"
     CDS             11852..13213
                     /gene="pabB"
                     /locus_tag="ACG0BW_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFK7259510.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NTRRAWLESQQFSPQEDFALTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HTTYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            13217..13795
                     /gene="nudL"
                     /locus_tag="ACG0BW_002561"
     CDS             13217..13795
                     /gene="nudL"
                     /locus_tag="ACG0BW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFK7259511.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            13979..15343
                     /gene="sdaA"
                     /locus_tag="ACG0BW_002562"
     CDS             13979..15343
                     /gene="sdaA"
                     /locus_tag="ACG0BW_002562"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFK7259512.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYKGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            15474..17072
                     /gene="pdeD"
                     /locus_tag="ACG0BW_002563"
     CDS             15474..17072
                     /gene="pdeD"
                     /locus_tag="ACG0BW_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFK7259513.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRVLIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPLTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPAQHNGHITPIMPLR"
     gene            complement(17076..18632)
                     /gene="yoaE"
                     /locus_tag="ACG0BW_002564"
     CDS             complement(17076..18632)
                     /gene="yoaE"
                     /locus_tag="ACG0BW_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFK7259514.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(18620..18778)
                     /gene="yoaL"
                     /locus_tag="ACG0BW_002565"
     CDS             complement(18620..18778)
                     /gene="yoaL"
                     /locus_tag="ACG0BW_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFK7259515.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            19095..20066
                     /gene="manX"
                     /locus_tag="ACG0BW_002566"
     CDS             19095..20066
                     /gene="manX"
                     /locus_tag="ACG0BW_002566"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFK7259516.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20129..20929
                     /gene="manY"
                     /locus_tag="ACG0BW_002567"
     CDS             20129..20929
                     /gene="manY"
                     /locus_tag="ACG0BW_002567"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFK7259517.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            20942..21793
                     /gene="manZ"
                     /locus_tag="ACG0BW_002568"
     CDS             20942..21793
                     /gene="manZ"
                     /locus_tag="ACG0BW_002568"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFK7259518.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            21848..22306
                     /gene="yobD"
                     /locus_tag="ACG0BW_002569"
     CDS             21848..22306
                     /gene="yobD"
                     /locus_tag="ACG0BW_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFK7259519.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         5391 a         5814 c         5610 g         5531 t
ORIGIN      
        1 actcattacc ctgcaaataa ttacaacagg caaaagccag agacgggctt ttatgcccag
       61 gagatgactc atgatgagca atgttaagaa aaaagatgtg ccgctgataa gcatcagcct
      121 agtggccatt cttttcatcg cagctgcatt aagccttttc ccacaacaat cggccgacgc
      181 ggccaacgca atatacactt ttgttacgcg tacgttaggt tccgccgtac aggtattggt
      241 tttgctggca atggggctgg tgatttattt agccaccagt aaatacggca atattcgtct
      301 tggcgaagga aaaccggaat acagcacgct ctcctggctg tttatgttta tttgtgccgg
      361 tttaggttct tctacgcttt attggggggt tgctgaatgg gcctattatt atcaaacacc
      421 tggattaaat atcgcaccgc gttcacaaca ggcactcgaa tttagcgtcc cttactcctt
      481 cttccactgg ggcatcagcg cctgggcaac ttatacgctg gcctcattaa tcatggctta
      541 tcactttcat gtgcggaaaa acaaaggtct gagtctttcc ggcattattg ccgcgattac
      601 cggcgttcgc ccgcaaggcc cgtggggaaa actggtcgat ttgatgttcc tgatcgccac
      661 tgtcggtgcg ctgaccattt cccttgttgt taccgcagca acctttaccc gtgggctttc
      721 cgcgctgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatac tgctttccgg
      781 cggcattttt tgcctaagct cgtggattgg tatcaacaac ggtttgcaac gtctgagcaa
      841 aatggttggc tggggcgcgt tcctgctgcc attactggtg ctcattgtcg gcccaaccga
      901 atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact tcctgcaaat
      961 gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga ccgttttcta
     1021 ctggctgtgg tggatctcat acacgcctgg cgtagcaatg tttgtcaccc gcgtttcccg
     1081 cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg tcggttgctg
     1141 gttcttcttc ggcgtaatgg aaagctatgc cattcaccag tttatcaata gcgtaatcaa
     1201 cgtcccacag gtgctggaaa cactgggcgg cgagacagct gtgcagcaag ttctgatgtc
     1261 gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta ttttccttgc
     1321 ctcgcatatg gatgcggtgg cctacaccat ggcggcaacc agtacgcgta atctccagga
     1381 aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtaatta ccctgatccc
     1441 gctttcaatt cttttcactg gcgcgtcgct ggaaacgatg aaaaccaccg tcgtactcac
     1501 agcccttccg ttcctcgtca ttttactggt gaaagtcggc ggatttattc gctggctgaa
     1561 acaggattac gccgacattc cggctcatca agttgaacat tatctcccgc agacaccggt
     1621 tgaagtcctg gaaaaaacgc cagtgctccc tgcgggaacc gtattcaaag gcgacaactg
     1681 agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct gactttgtac
     1741 tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc cgtttttata
     1801 ccgatcagaa cgcctttgaa cacgaaaaag agagcgtctt cgccaaaagc tggatttgcg
     1861 tcgctcatag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag atcattggcg
     1921 aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc
     1981 cgcaccgtgg tcatcagtta ttgagcggcg aagggaaagc gaaaaatgtg attacctgcc
     2041 cgtatcacgc atgggcattc aaactcgatg gcaacctggc ccatgcacgt aactgcgaaa
     2101 acgtcgccaa tttcgatagc gacaaagctc aactggttcc ggtgcgtctg gaagaatatg
     2161 ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat cagttacccg
     2221 gtctgggggc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc
     2281 gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat ctcgagtgct
     2341 atcactgtgg tccggcgcat ccaggttttt ccgactccgt tcaggttgat cgttactggc
     2401 acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc gaacagtctt
     2461 tcaaattcga agagggcacg gatgcggcat tccacggttt ctggctgtgg ccgtgcacga
     2521 tgctgaacgt cactccgatc aaagggatga tgacggtcat ttatgaattc ccggtggatt
     2581 ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta accgacgagc
     2641 aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta cgtctggttg
     2701 aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg
     2761 acagtagcgg tagtggcatt tccgaacatg gtatcgccca tttccataat ctgctggcgc
     2821 aggtatttaa ggactaatga catcggcggc ggtattttcc gccgctgggc tgatttttga
     2881 tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagcca ggttgaaccg
     2941 cttaccgaac aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc attacctgcg
     3001 tttaccggag gaagtcacat cattgtgcag atgagcgatg gtgataacca gtacagcaat
     3061 gcttattcac tcctgagctc gccgcatgac acctctagtt atcagattgc cgttcggctg
     3121 gaagaaaact cgcgcggcgg ttcacgcttt ttacatcagc aggtaaaagt gggcgatcgg
     3181 ttaacgattt caacgcctaa taacctgttt gcgctaattc cctcggccag aaagcatctg
     3241 tttatcgcgg gcggcattgg tatcacccct ttcctgtcgc acatggcaga gttgcaacac
     3301 agcgacgtcg actggcagct acattactgc acgcgaaatc cagaaagttg cgcatttcgt
     3361 gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc atcaaccgga
     3421 acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca tgtttatacc
     3481 tgtggccccg aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc
     3541 gccgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga tgcatttacc
     3601 ctggtgcttg cccgttccgg taaagagttt gtggtgccgg aagagatgac tattttgcag
     3661 gttattgaaa ataataaagc agcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga
     3721 acctgcgaaa cggcaatact ggaaggtgaa gctgaccatc gggatcagta ttttagcgat
     3781 gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgcgcgaa gggtaaacgc
     3841 ctggtgttgg atttgtagtt tgccgaagcc ggatgtggcg ctgagcgcgc ccagtccggc
     3901 ttcggaagat tttactgcgg atattcctgc aataaattgt gtaatgcttc cgccatcagc
     3961 tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag tttccagtgc
     4021 cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct gatcttgtgc
     4081 gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg ataacccggc
     4141 atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt
     4201 tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga taagcccagg
     4261 ctgtccagtt cgcctaaact tcccggcata taacgcgcta ccgaccacaa atgctcttca
     4321 cgcacgacaa agttcaccgc cagatcgcgc tcccgcgcct tgcgcagtcg ccagtcggct
     4381 aacagttgca gacaggccag ttggcgtgtg cgtaactgcc aggcattggt gatatcacgc
     4441 caggcatctt ccggtgcaac gacttcctga cgacgcattt gcatcaggcg gcattcatcc
     4501 agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagcttggc ggtgatcggt
     4561 aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag cggtctggcc
     4621 agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc ttccaccatg
     4681 gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc
     4741 aggggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc actgcctgca
     4801 tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg
     4861 atcccgagag gatcaattag cgccagatgc tcaccatcaa aaagttgaat tagacccagt
     4921 tgcgggtaat aggtgcgcgt acgaacaaat tcagtatcca gggctatcgc cggaaaggca
     4981 cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg gtaattcaaa
     5041 ttgtattctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga
     5101 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     5161 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     5221 ttgtagcccg tgagctgacg gcggcaaaag gtcaccagtg actcttcggt aagcgatgga
     5281 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     5341 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     5401 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     5461 tcttcatcca tcaccgcgat gtcgccagtg tgtaaccagc catttttgat gatctcatcg
     5521 gtagcatccg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctcg
     5581 cccggttgac ccggtggtac ttcgttgtcg tcgtcatcca ccagtttggc ttccgtcgac
     5641 ggcaccggca aaccgatgct accactatga taatcaatat catacgggtt aacgctcacc
     5701 agcggcgcac actcggtcag gccataacct tccagcagat actgtccagt cagtttcacc
     5761 caacgttctg ccaccacttg ctgaacaggc atccctccgc ctgcggaaag atgcagactg
     5821 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     5881 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaacccagg aatatcgcgc
     5941 ggattagtga taagcaggtt ctgccccccc agttcgataa acagcaggca gttaatggtc
     6001 agggcaaaaa tgtgatacag cggcagcgcc gtcaccacca gctctttgcc cggatgcaac
     6061 agcggaccat aggtcgcgtt aacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     6121 atcgcgcctt tcgccacacc agtggtaccg ccggtgtatt gcaggaaagc taaatcttcc
     6181 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tgtgcagggc gctacgaaat
     6241 gaaatggcat ctggcagatg gtatttcggc accagacgct tgatgtattt aacaacgaaa
     6301 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     6361 tgaacggcgg ttttatcaac cactttttcc agagtgtggg caaagttaga cacgataaca
     6421 atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     6481 ttaacgttta caacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     6541 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagtcccaa cccttgttgc
     6601 aaataagcgg caaacgcgcg actgcgttct tccagtttgc ggaaggtcat cacctccccc
     6661 atattcacaa acgcgggttg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     6721 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     6781 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     6841 gatatacatg tcgttaacat aatattaact catcatacca gcttgataat tacccaacga
     6901 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     6961 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aacagtttgt
     7021 actctcgcgg ggccgggatt ataccagccc catccaccgt agccataggg ccagccacga
     7081 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     7141 caacgtttgt aacccgttac ctgcatcacc ataaatttat agggcgtgtt gccgattttg
     7201 ccgtcaaccg caccggtgat tggcccaact accgtaacca gttgtccacg gaaatctacc
     7261 gggtccagaa aaccgttcac atcagcataa atgcgaccgc gagaaggttc tcccagcgtc
     7321 ggtctggctc cgctgtccag tggtacggtg gcaatttcca ggcgggtttt cccttgctgg
     7381 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     7441 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgctgccttt aatggcgtcc
     7501 ggcacagtaa cacaaccgct gagcattagc gcaaacgtac ctgccagtat gcctttgata
     7561 acattctttt gaaccgccat gatgcgactc cttttttctc agggcatact cttaagattc
     7621 attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat
     7681 gttcaaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcgga agcatatctt
     7741 ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttaccgagat
     7801 ccggccaggc ttgccagcct gtacctaccg tcacccattc gccggaaagc tgttgcatcc
     7861 gttcatgaac gagttcgggt ttgagtacag cttcggtttc ttcaccgtgc cagataccgt
     7921 tttcatcacg ctgatattcg gcccagtaaa cttcgcccat ccgcgcatca atagcagcca
     7981 gcacgcgggt tgcgccgttt ttgcgccacg ccccttgcgc cattgtcatt agcgtggaga
     8041 cgccaatcat cggtaattcc gcgccaagtg ccagcccttg cgcaatgcca ataccaatgc
     8101 gcacgccggt aaagctaccg gggccgcgcc cgtatgccag agcatttatg tcagtcaggg
     8161 aagttccgct ggtggtcagg atatcctgca ccatcggtaa aattcgttga gtatgttcac
     8221 gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg acagagcagg
     8281 cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa
     8341 aggcgcgcat cataccatac tccatcacaa attacctgga ggatggtatc gcaaggaaac
     8401 gaaccgcacg ggcaatgtca cgggtgcgcg gcgctggcgg cagactggcg agaaacgtcg
     8461 cgccgtaagg gcgcatcacc agccgattat cgcaaatcac caaaacccct cgatcgtcgg
     8521 cgtcgcgaat tagtcgccct accccctgtt tgagagtaat gaccgcatcc ggcagctgta
     8581 cttcatcgaa cgggtcgcct ccgcgcaaac ggcaatcttc catgcgcgct tttaatagtg
     8641 gatcatccgg tgaggtaaac ggcaacttgt cgataatcac caatgacaat gtatcgccac
     8701 gcacatccac cccttcccag aagctgctgg ttgccacaag aagcgcgtta ccggcgctga
     8761 caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca
     8821 tggtagcgcg gaactgctca gccagatcgc gcatcatggc gtgcgaggta caaagcataa
     8881 aacaacgacc gttgttagct tcgatgatcg gtcgcaacat tgccgccagt tgccgtgcgg
     8941 agcccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc tggcggctgt
     9001 aatcaaacgg gctgggtaac agcaacgact ctgcctgttc aatgccaagc cgcgaggtaa
     9061 aatgatgcag atcgtcattc accgacagcg ttgctgaggt aaagatccag ctacctggtt
     9121 tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgagt gccagagtaa
     9181 aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc
     9241 gtttcagccg cgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc aaggcggaac
     9301 gccccagcga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa
     9361 acgcccgctg aatttgcgga ttagctaaca gttcgcgcag gttgccacga taacctggct
     9421 cacctaattg cagacgaaaa tcctgcgcac tctgggcaag acgatcagtg cacttttgta
     9481 actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt gccaggtcga
     9541 gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc ggtagctggt
     9601 gggcttcgtc gaagatcatc acgtccgctt ccgggatcaa ctcgccaaat ccactctcct
     9661 taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg
     9721 cttttttgcg tgctttgacc acaaagcatt ctttatacat cgggcagtcg ctgccaaggc
     9781 agttatcgtt ggtactggtg accagcggcc acgcctgtga atcttccgcc acgctgacgc
     9841 aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc
     9901 ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgctcgaggc
     9961 agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg
    10021 agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgaa ccggtcgaga
    10081 taatgacttt ctttttcgcc cgcagcgcag gggccaggta agcgtaggtt ttgcccgtac
    10141 cggttccggc ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg
    10201 ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc aactgaccgt
    10261 ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga
    10321 tgagggcgct ttcgccagtt gaagtgatga cggcgacctc acattgtggc agtctttgca
    10381 gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa
    10441 gcccgctggt ctgatgtggt gatccacaac aacacgctct actacactgg tgtaccggaa
    10501 aacctcgacg ccgatgcttt cgagcaaact gccaacacgc tggcgcagat tgacgccgtg
    10561 ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt cctggccgat
    10621 aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg
    10681 ccggtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt tgaaattaag
    10741 attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata
    10801 atactggtcg gttttacata aatcgaccag agattaagat attactgttg atgctcctgc
    10861 taattcatta tttattttta tattttcaat tttagctata aagctaacta tataaataaa
    10921 cataaccatg ccgttgtgtt cgcccttcga ttagcatctc tggttttctt tcttagctga
    10981 acaatttggt tatagcgtga gaactgaccg tcaattttct ctctcttact ggataacaca
    11041 ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg ccatatcgtt
    11101 ataatccctt tgatattaga tgcaaattaa ggtcatatat agccttatcg ataacatggt
    11161 taattttaag gataagaata tgcctgctgt aatagataaa gccctggatt tcattggtgc
    11221 catggatgta tcagcaccaa caccaagttc gatgaatgaa agcacggcga agggaatctt
    11281 taaatattta aaagaactgg gagtacccgc gagcgcagcc gatattacgg cgcgagctga
    11341 ccaggaaggc tggaacccgg ggttcacgga aaaaatggtt ggatgggcaa aaaaaatgga
    11401 gtctggtgaa cgcattgtga ttaaaaatcc tgaatacttt tcaacatata tgcaggaaga
    11461 actaaaagca ctggtctaag ttaaatttat atcagcataa atgggtcagg acgcttttaa
    11521 gcacatatga aaagcgtcct ttttctcaca accaaataac cattgacttc tataacgaaa
    11581 gcgatacgga taagtgtgtt actcgtcttc atcctcaaaa cgcgccacga ttcgctcgcc
    11641 ggaatggttg gcacgcagct cttctgccac caacgcgatt gcctggccgc tgctcatccc
    11701 ttgtgccatc agttcctgga tccgctcgac ggctttttgc tgctgttcat gggtgagtga
    11761 aggtaaacct gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt atttcatgct
    11821 acccggcaca tagccagtag agtcaggact gatgaagacg ttatctcccg ctgtgattac
    11881 tttaccctgg cgtcaggacg ccgctgaatt ttatttctca cgcttaagcc acctgccgtg
    11941 ggcgatgctt ttacactccg gctatgccga tcatccgtat agccgctttg atattgtggt
    12001 cgccgagccg atttgcactt taaccacttt cggtaaagaa accgttgtta gtgaaagcga
    12061 aaaacgcaca acgaccactg atgacccgct acaggtgctc cagcaggtgc tggatcgcgc
    12121 agacattcgc ccagcgcata acgaagattt gccatttcag ggcggcgcgc tggggttgtt
    12181 tggctacgat ctgggccgcc gttttgagtc actgccagaa attgcgcagc aagatatcgt
    12241 tctgccggat atggcagtgg gtatctacga ctgggcgctg gttgttgacc accagcgtca
    12301 aacagtttct ttgctgagtc ataatgatgt caatacccgt cgggcctggc tggaaagcca
    12361 gcaattctcc ccgcaggaag atttcgcgct cacatccgac tggcaatcca atatgacccg
    12421 cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat ctgcacagtg gtgattgcta
    12481 tcaggtgaat ctcgcccagc gttttcatac gacctattct ggcgatgaat ggcaggcatt
    12541 ccttcagctt aatcaggcca accgcgcgcc gtttagcgct tttttacgtc ttgaacaggg
    12601 tgcgatttta agcctttcgc cagagcggtt tatcctttgt gataatagtg aaatccagac
    12661 ccgcccgatt aaaggcacgc taccacgcct gcccgatcct caggaagata gcaaacaagc
    12721 agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat ctgatgattg tcgatttaat
    12781 gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta aaagtaccag agctgttcgt
    12841 ggtcgaaccc ttccctgccg tgcatcatct ggtcagcacc ataacggcgc ggctaccaga
    12901 acagttacac accagcgatc tgctgcgcgc agcttttcct ggtggctcaa taaccggggc
    12961 tccgaaagta cgggctatgg aaattatcga cgaactggaa ccgcaccgac gcaatgcctg
    13021 gtgcggcagc attggctatt tgagcttttg cggcaacatg gacaccagca ttactatccg
    13081 cacgctgact gccattaacg gacaaattta ctgctctgcg ggcggtggaa ttgtcgccga
    13141 tagccaggaa gaagcggaat atcaggaaac ttttgataaa gttaataaga tattacgcca
    13201 actggagaag taagacgtgg aataccgtag cctgacgctt gatgattttt tatcgcgctt
    13261 tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat catcgtcagg ctgccgtgtt
    13321 aatccccatc gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt cgattcatct
    13381 gcgtaaacac gctggacaag tggcattccc tggaggcgca gtcgatgaca cggacgcatc
    13441 agttatcgcc gctgcgctgc gcgaagctga agaagaggtc gctataccgc cttccgccgt
    13501 tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact ggctatcagg taaccccggt
    13561 ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag tctcggcggt
    13621 gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcaccctt tagatatcta
    13681 ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt ttgtatgggg
    13741 aatgaccgca ggcataattc gtgaactggc gttgcaaatt ggtgtgaaac cctgactata
    13801 cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta gttaaatcgc
    13861 ggtttttgat tagtttaatt catgtgaata gttaagccag tcgccgcgtt ccctcttaca
    13921 ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga gtattatcgt
    13981 gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc ataccgtagg
    14041 gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct tactggatag
    14101 cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta aaggccacca
    14161 caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg tggatatcga
    14221 cagtattccc ggttttattc gcgacgtaga agagcgcgaa cgtctgctgc tggcacaggg
    14281 acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg gcaacctgcc
    14341 gctgcatgaa aacggtatgc aaatccacgc ctataaaggc gatgaagtcg tctacagcaa
    14401 aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg gtcaggatgc
    14461 cgccaacgaa gtgagcgtgc cgtatccttt caaatctgcc accgaactgc tcgcgtactg
    14521 taatgaaacc ggctattcgc tttctggtct cgctatgcag aacgaactgg cgctacacag
    14581 caagaaagag atcgacgagt atttcgctca tgtctggcaa accatgcagg catgtatcga
    14641 tcgcgggatg aataccgaag gcgtactgcc aggcccgctg cgcgtgccgc gtcgtgcgtc
    14701 ggccctgcgc cggatgttag tttccagcga taaactgtct aacgatccga tgaatgtcat
    14761 tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg gtggtcgtgt
    14821 ggtaactgcg ccaaccaacg gtgcttgtgg cattgttccg gcagtgctgg cttattatga
    14881 ccactttatt gaatcggtta gcccggacat ctatacccgt tactttatgg cagcgggcgc
    14941 gattggtgca ctgtataaaa tgaacgcgtc tatttccggt gcggaagtcg gttgccaggg
    15001 cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt gcagaactgc tgggcggtag
    15061 cccggaacag gtttgcgtgg cggcggaaat tggcatggaa cacaaccttg gtttaacctg
    15121 cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag cgtaatgcca ttgcctctgt
    15181 gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc gcgtctcgct
    15241 ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcca aataccgcga
    15301 aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct tactcgccca
    15361 tctgcaacgg atgggcgaat ttatccccgc tttctcgtct gctgtaattt tccccactac
    15421 acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc cgcatgcaaa
    15481 aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt acgatttgcg
    15541 cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt aacttaaatc
    15601 aacaacgggt agtacaattc gctaatcacg ctgtagagga attagataaa gtactgcttc
    15661 ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc tctgtcgccc
    15721 atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt ggcctggtgc
    15781 aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc gtcgtggaca
    15841 ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgatcgac cgtgtcctga
    15901 ttaaaggcag tccggttttg attcaatgga cacctgcagc gggcagtagc aatgctgggg
    15961 tcatggagat gattaacatc gacttactga cggcaatgct ccttgagcca caactgccgc
    16021 aaatcagtag cgccagcctg acggtggaca aacggcattt gctctatggt aatgggctgg
    16081 tagattccct tccgcaacct gaaaacaatg aaaactatca ggtttcttcg caacgctttc
    16141 ctcttaccat taacgttaat ggtccggggg ctacggcgct ggcatggcac tatcttccaa
    16201 cacaattacc gctggcggtg ctgctaagtt tactggtggg ctatatcgcc tggctggcga
    16261 ccgcttaccg gatgagtttt tcccgcgaaa tcaatctggg cctggcgcaa catgagttcg
    16321 aattgttctg ccagcctttg cttaatgcgc gcagccagca atgtattggt gtagagattt
    16381 tgctgcgctg gaacaatccg cgtcagggct ggatttcacc ggatgtgttt attcctatcg
    16441 cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa accatccgtc
    16501 agcggcatgt tttcccaatg agtagtcagt ttcatgttgg cattaacgtc gcacccagtc
    16561 attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc gctcacccga
    16621 ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt gattatcgga
    16681 ttgcccgcga gctgcatcgt aaaaacgtca aactggcgat tgatgacttc ggcaccggca
    16741 acagttcgtt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt gataagtcat
    16801 ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc atcatcgcgc
    16861 tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa gaacaggcga
    16921 agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca cagccgatgc
    16981 cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc cagcataatg
    17041 gacatatcac gcccattatg ccgttacgtt aacgcttact catcttcatc gtgcgccggt
    17101 tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaat gatggtgata
    17161 tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt tgccgagatc
    17221 acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc
    17281 tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgtcacc atcggtaatg
    17341 atttccggcg tttcgtcagc gtccgggaat tcacccgcaa tggcttccag cacatccagc
    17401 ggcgtgacca gaccttgtac cacaccaaac tcgttggtca cgataacaaa gctcccgcga
    17461 gcacgacgca gcacgcccag caggttgatc ggatcgaggg tttcagggac gataatcgcc
    17521 ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac cagcagttct
    17581 ttagcacgta cgattccgat gatttcatcc agttcaccgc gacataccgg gaacaggctg
    17641 tgcggtgagg agagcagctg ctcgcggatt tcatcgaccc cgagattagc gtcaacccag
    17701 cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag cgtgagtacg
    17761 ccgttaatca tgtaacgttc ttcttcggca aacgcacctt ccgggatcgg catcggcatc
    17821 gggttatcgg catcgtgctg aacattggcc tgacgtttcc cgcccatcaa acgcaggatg
    17881 gcatcggcag tacgcgctcg cagcggcaaa gtcgactggt gacgaataaa gttgcgacgc
    17941 gcaatctggt taaacacttc gatgatgatc gagaagccaa tcgcggcata caggtaacct
    18001 ttcggaatgt ggaaaccgaa accttctgcc accagactca gacctatcat taacaggaag
    18061 ctcagacaga gcaccaccac cgtgggatgc tggttaacaa atcgcgtcag cggtttcgat
    18121 gccagcagca taaccgccat cgcaatcact accgccgcca tcatcactgg cagatggtta
    18181 accatcccta ctgccgtaat taccgcatcc aatgagaaga cggcgtcaag gatgacaatc
    18241 tgtgtgacga ccacccagaa actggcgtag cctttaccgt ggccggaatc atgatcgcgg
    18301 ttttccagcc gttcatgcag ttcggttgtt gctttgaaca gcaagaatat ccccccgaac
    18361 aacataatca ggtcgcgtcc ggagaaggag aaatccatga cggtaaatag cggtttggtc
    18421 agcgtgacca tccatgaaat cagcgacagc agccccagac gcataatcag cgccagtgat
    18481 aatcccagca aacgcgcttt atcgcgttgt tttggcggca gtttgtcagc aagaatggcg
    18541 atgaagacca ggttatcgat acccagcaca atttcgagaa caacaagcgt gagtagcccc
    18601 gcccaaattg aggggtccat taagaattcc atgacaagct cctgcttaag gaatagctat
    18661 tcgacgccag aaataatgca ggcgtaacga caaaatgcaa acgaaaggtg cggcatagag
    18721 tgccagaaag gcaggcgtta aaaggcctga tgctgaaatg acgtcggtga cgatccatac
    18781 tgcgggctac tgccctatac tccatggttg ttaaacggga gttaaacata tcagagacgc
    18841 ctctgatttg gcaaagattt accttccttt gcaaacgaat gtgacaagga tattttacct
    18901 ttcgaaattt ctgctaatcg aaagttaaat tacggatctt catcacataa aataattttt
    18961 tcgatatcta aaataaatcg cgaaacgcag gggtttttgg ttgtagccct tatctgaatc
    19021 gattcgattg tggacgacga ttcaaaaata catctggcac gttgaggtgt taacgataat
    19081 aaaggaggta gcaagtgacc attgctattg ttataggcac acatggttgg gctgcagagc
    19141 agttgcttaa aaccgcagaa atgctgttag gcgagcagga aaacgtcggc tggatcgatt
    19201 tcgttccagg tgaaaatgcc gaaacgctga ttgaaaagta caacgctcag ttggcaaaac
    19261 tcgacaccac taaaggcgtg ctgtttctcg ttgatacatg gggaggcagc ccgttcaatg
    19321 ctgccagccg cattgtcgtc gacaaagagc attatgaagt cattgcaggc gttaacattc
    19381 caatgctcgt ggaaacgtta atggcccgtg atgatgaccc aagctttgat gaactggttg
    19441 cgctggcagt agaaacaggc cgtgaaggcg tgaaagctct gaaagccaaa ccggttgaaa
    19501 aagccgcgcc agcacccgct gccgcagcac caaaagcggc tccaactccg gcaaaaccaa
    19561 tgggaccaaa cgactacatg gttattggcc ttgcgcgtat cgacgaccgt ctgattcacg
    19621 gtcaggtcgc cacccgctgg accaaagaaa ccaatgtctc ccgtattatt gttgttagtg
    19681 atgaagtggc tgcggatacc gttcgtaaga cactgctcac ccaggttgca cctccgggcg
    19741 taacagcaca cgtagttgat gttgccaaaa tgattcgcgt ctacaacaac ccgaaatatg
    19801 ctggcgaacg tgtaatgctg ttatttacca acccaacaga tgtagagcgt ctcgttgaag
    19861 gcggcgtgaa aatcacctct gttaacgtcg gtggtatggc attccgtcag ggtaaaaccc
    19921 aggtgaataa cgcggtttcg gttgatgaaa aagatatcga ggcgttcaag aaactgaatg
    19981 cgcgcggtat tgagctggaa gtccgtaagg tttccaccga tccgaaactg aaaatgatgg
    20041 atctgatcag caaaatcgat aagtaacgta ttgtgttgat tatcactcag ttttcacact
    20101 ttagtcttac gtaaacagga gaagtacaat ggagattacc actcttcaaa ttgtgctggt
    20161 atttatcgta gcctgtatcg caggtatggg atcaatcctc gatgaatttc agtttcaccg
    20221 tccgctaatc gcgtgtaccc tggtgggtat cgttcttggc gatatgaaaa ccggtattat
    20281 tatcggtggt acgctggaaa tgatcgcgct gggctggatg aacatcggtg ctgcagttgc
    20341 gcctgacgcc gctctggctt ccatcatttc taccattctg gttatcgcag gtcatcagag
    20401 cattggtgca ggtattgcac tggcaatccc tctggccgct gcgggccagg tactgaccat
    20461 catcgttcgt actattaccg ttgctttcca gcacgctgcg gataaggctg ctgataacgg
    20521 caacctgaca gcgatttcct ggatccacgt ttcttctctg ttcctgcaag caatgcgtgt
    20581 ggctattccg gccgtcatcg ttgcgctgtc tgttggtacc agcgaagtac agaacatgct
    20641 gaacgcgatt ccggaagtgg tgaccaatgg tctgaatatc gccggtggca tgatcgtggt
    20701 ggttggttat gcgatggtta tcaacatgat gcgtgctggc tacctgatgc cgttcttcta
    20761 cctcggcttc gtaaccgcag cattcaccaa ctttaacctg gttgctctgg gtgtgattgg
    20821 tactgttatg gcagtgctct acatccaact tagcccgaaa tacaaccgcg tagccggtgc
    20881 gcctgctcag gcagctggta acaacgatct cgataacgaa ctggactaac aggtgagcga
    20941 aatggttgat acaactcaaa ctaccaccga gaaaaaactc actcaaagtg atattcgtgg
    21001 cgtcttcctg cgttctaacc tcttccaggg ttcatggaac ttcgaacgta tgcaggcact
    21061 gggtttctgc ttctctatgg taccggcaat tcgtcgcctc taccctgaga acaacgaagc
    21121 tcgtaaacaa gctattcgcc gtcacctgga gttctttaac acccagccgt tcgtggctgc
    21181 gccgattctc ggcgtaaccc tggcgctgga agaacagcgt gctaatggcg cagagatcga
    21241 tgacggtgct atcaacggta tcaaagtcgg tttgatgggg ccgctggctg gtgtaggcga
    21301 cccgatcttc tggggaaccg tacgaccggt atttgcagcg ctgggtgccg gtatcgcaat
    21361 gagcggcagc ctgttaggtc cgctgctgtt cttcatcttg tttaacctgg tgcgtctggc
    21421 aacccgttac tacggcgtag cgtatggtta ctccaaaggt atcgatatcg ttaaagatat
    21481 gggtggtggt ttcctgcaaa aactgacgga aggggcgtct atcctcggcc tgtttgtcat
    21541 gggggcatta gttaacaagt ggacgcatgt caacatcccg ctggttgtct ctcgcattac
    21601 tgaccagacg ggcaaagaac acgttaccac tgtccagact attctggacc agttaatgcc
    21661 aggcctggta ccactgctgc tgacctttgc ttgtatgtgg ctactgcgca aaaaagttaa
    21721 cccgctgtgg atcatcgttg gcttcttcgt catcggtatc gctggttacg cttgcggcct
    21781 gctgggactg taagactgtt gtacactacc ggggcctttt ggccccgttt ttttatctgg
    21841 aggattaatg acaatcacgg acctggtact gattcttttc atcgccgcac tcctggcctt
    21901 cgcgatctac gatcagttca tcatgccccg ccgtaacggc cccaccctgc tggcaattcc
    21961 tttgctccgg cgtggtcgca tcgatagcgt tatcttcgtc ggattgattg tcattcttat
    22021 ctataacaac gtcacgaatc atggcgcgct aataacaaca tggttattaa gtgcactggc
    22081 tctgatgggt ttttatatat tctggatccg cgttccgaag atcatcttta aacaaaaagg
    22141 ttttttcttc gccaatgtct ggattgaata tagccgaatc aaagcgatga acttgtcgga
    22201 agatggcgtg ttggtgatgc aattagaaca gcgtcggctg ttaatccgcg ttcgaaatat
    22261 cgacgatctg gaaaaaattt ataagcttct cgtttcaact caataagtta tgaatttagc
    22321 caaagctatg tttagtgtat ttttaa
//
LOCUS       DAYPHN010000071        22232 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000071 DAYPHN010000000
VERSION     DAYPHN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.071"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..871
                     /gene="ybeF"
                     /locus_tag="ACG0BW_002570"
     CDS             <1..871
                     /gene="ybeF"
                     /locus_tag="ACG0BW_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFK7259520.1"
                     /translation="IDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFP
                     DPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGAL
                     VLPVIYRAIKSHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDN
                     MVLICREGNPLLSLEDDRETIDNAAHILLLPEGQNFSGLRQRVQEMFPDRQISFTSYN
                     ILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDP
                     ILHGVIDVIRNAF"
     gene            1080..2045
                     /gene="lipA"
                     /locus_tag="ACG0BW_002571"
     CDS             1080..2045
                     /gene="lipA"
                     /locus_tag="ACG0BW_002571"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFK7259521.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2146..2349)
                     /gene="tatE"
                     /locus_tag="ACG0BW_002572"
     CDS             complement(2146..2349)
                     /gene="tatE"
                     /locus_tag="ACG0BW_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFK7259522.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2478..3266)
                     /locus_tag="ACG0BW_002573"
     CDS             complement(2478..3266)
                     /locus_tag="ACG0BW_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFK7259523.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3359..3742
                     /gene="crcB"
                     /locus_tag="ACG0BW_002574"
     CDS             3359..3742
                     /gene="crcB"
                     /locus_tag="ACG0BW_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFK7259524.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRLGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3796..4005)
                     /gene="cspE"
                     /locus_tag="ACG0BW_002575"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3796..4005)
                     /gene="cspE"
                     /locus_tag="ACG0BW_002575"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFK7259525.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4180..4740)
                     /gene="pagP"
                     /locus_tag="ACG0BW_002576"
     CDS             complement(4180..4740)
                     /gene="pagP"
                     /locus_tag="ACG0BW_002576"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFK7259526.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFGENITQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5329..6714
                     /gene="dcuC"
                     /locus_tag="ACG0BW_002577"
     CDS             5329..6714
                     /gene="dcuC"
                     /locus_tag="ACG0BW_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFK7259527.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6755..7435)
                     /gene="dpiA"
                     /locus_tag="ACG0BW_002578"
                     /gene_synonym="citB"
     CDS             complement(6755..7435)
                     /gene="dpiA"
                     /locus_tag="ACG0BW_002578"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFK7259528.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPIGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(7404..9062)
                     /gene="dpiB"
                     /locus_tag="ACG0BW_002579"
     CDS             complement(7404..9062)
                     /gene="dpiB"
                     /locus_tag="ACG0BW_002579"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFK7259529.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            9442..10500
                     /gene="citC"
                     /locus_tag="ACG0BW_002580"
     CDS             9442..10500
                     /gene="citC"
                     /locus_tag="ACG0BW_002580"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFK7259530.1"
                     /translation="MFGNDICTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            10515..10811
                     /gene="citD"
                     /locus_tag="ACG0BW_002581"
     CDS             10515..10811
                     /gene="citD"
                     /locus_tag="ACG0BW_002581"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFK7259531.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            10808..11716
                     /gene="citE"
                     /locus_tag="ACG0BW_002582"
     CDS             10808..11716
                     /gene="citE"
                     /locus_tag="ACG0BW_002582"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFK7259532.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGIELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            11727..13259
                     /gene="citF"
                     /locus_tag="ACG0BW_002583"
     CDS             11727..13259
                     /gene="citF"
                     /locus_tag="ACG0BW_002583"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFK7259533.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIFNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            13263..13814
                     /gene="citX"
                     /locus_tag="ACG0BW_002584"
     CDS             13263..13814
                     /gene="citX"
                     /locus_tag="ACG0BW_002584"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFK7259534.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            13789..14667
                     /gene="citG"
                     /locus_tag="ACG0BW_002585"
     CDS             13789..14667
                     /gene="citG"
                     /locus_tag="ACG0BW_002585"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFK7259535.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            14717..16180
                     /gene="citT"
                     /locus_tag="ACG0BW_002586"
     CDS             14717..16180
                     /gene="citT"
                     /locus_tag="ACG0BW_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFK7259536.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            16294..17100
                     /gene="rna"
                     /locus_tag="ACG0BW_002587"
     CDS             16294..17100
                     /gene="rna"
                     /locus_tag="ACG0BW_002587"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFK7259537.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGNTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            17330..17740
                     /gene="rnk"
                     /locus_tag="ACG0BW_002588"
     CDS             17330..17740
                     /gene="rnk"
                     /locus_tag="ACG0BW_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFK7259538.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            17883..17972
                     /gene="yldA"
                     /locus_tag="ACG0BW_002589"
     CDS             17883..17972
                     /gene="yldA"
                     /locus_tag="ACG0BW_002589"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFK7259539.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            18096..18524
                     /gene="uspG"
                     /locus_tag="ACG0BW_002590"
     CDS             18096..18524
                     /gene="uspG"
                     /locus_tag="ACG0BW_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFK7259540.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(18645..20210)
                     /gene="ahpF"
                     /locus_tag="ACG0BW_002591"
     CDS             complement(18645..20210)
                     /gene="ahpF"
                     /locus_tag="ACG0BW_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFK7259541.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVEIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(20381..20944)
                     /gene="ahpC"
                     /locus_tag="ACG0BW_002592"
     CDS             complement(20381..20944)
                     /gene="ahpC"
                     /locus_tag="ACG0BW_002592"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFK7259542.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            21316..22062
                     /gene="dsbG"
                     /locus_tag="ACG0BW_002593"
     CDS             21316..22062
                     /gene="dsbG"
                     /locus_tag="ACG0BW_002593"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFK7259543.1"
                     /translation="MLKKILLLALLPAIAFAEELPDPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         5460 a         5740 c         5362 g         5670 t
ORIGIN      
        1 tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag ggatcgttaa
       61 tgcagcgaaa gtgcttaatc tgacaccctc ggcaatcagt cagtctattc agaaactgcg
      121 cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc ctaccgcatt
      181 tgcgatgcat ctacatgagt atatcagtca gggccttgag tccattcttg gcgcgctgga
      241 tatcgaagga agctatgata agcaacgaac gataactatt gcgaccaccc cctcggtcgg
      301 agccctggtc cttcctgtca tctatcgggc gattaaatct cactatccgc agcttttact
      361 gcgcaacccc cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat
      421 catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa
      481 tatggtgtta atttgccgcg agggaaatcc actactctct ttagaagatg acagggagac
      541 tatcgacaac gctgcgcata tactcctgtt accggaaggg caaaatttca gcggtctgcg
      601 gcagagagtt caagagatgt ttccggaccg gcaaatcagt ttcaccagct acaacatttt
      661 gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca gccgttttta
      721 taacctgttt agccgttgct ggccgctgga aaaactgcct tttccgtcct taaatgagga
      781 gcaaatagat ttctctatcc actacaacaa attcagcctg cgtgatccca tcctgcacgg
      841 ggtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca gatcacagcg
      901 aagaacgaaa aaatggcaca atccaacaac aattttacat tttcgcgacc gctttggctg
      961 ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag ttgataatta
     1021 caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac acgcacgcta
     1081 tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat aagatggccc
     1141 ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag ccggaatgga
     1201 tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc gcaatgcgca
     1261 aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg gaatgcttca
     1321 accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt tgtccgttct
     1381 gtgacgttgc ccacggtcgc ccggtagctc cggatgccaa tgaaccagtg aaactggcgc
     1441 agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac cgtgatgacc
     1501 tgcgcgatgg cggtgcccag cactttgcgg attgcattac tgccattcgg gaaaaaagcc
     1561 cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg tcgtatggat cgtgctctgg
     1621 atattctgac cgccacacca ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta
     1681 tttaccgtca ggtacggcct ggtgcagatt acaactggtc gctgaagctg ctggaacgct
     1741 ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtgggt ctgggtgaaa
     1801 ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa
     1861 cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc
     1921 cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc catgctgcat
     1981 gcggtccgtt tgtccgctcg tcttaccacg ccgatttgca ggcgaaaggg atggaagtta
     2041 agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt tgaagcgggt
     2101 tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact ctttatgaga
     2161 gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat cgtcatcatt
     2221 catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg tacgtaactt
     2281 cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg taatactaat
     2341 ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt aaaaaccaga
     2401 gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag gtgaatcaag
     2461 cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt gtttaagacg
     2521 ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat aatgagtgca
     2581 ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg gctttgcccg
     2641 atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc cagcgcacga
     2701 gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca ggcggcagga
     2761 agtaccagga tttcagctcc ctgtaatgcc tgtgccagcg ccagctctgg aaagcgtaag
     2821 tcataacagg tcatcagacc gaccttcatc ccttccacct ccagtaacgg agctatttca
     2881 ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag atgcagtttg
     2941 gcataacggg cgacgatgtt tcctgcctgt agcgccacca gcatattcca tgcgcgcccc
     3001 ggcgttgaag gaacatgaat cgtcagaatt gtcgtcatca tgttacgttt actttctcgc
     3061 cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga tagatctgca
     3121 tcatgatcat cacgcgccaa caatgcttcc ggcaggacaa acagcgaaac gtcgttttcc
     3181 gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac agatgtaaca
     3241 gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc agagtcgtaa aattcgccaa
     3301 cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg attttgcagt
     3361 gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtgg cgagatggct
     3421 gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc tggctgcaaa
     3481 cctgattggg gcattcatca taggaatggg attcgcctgg tttagcagga tgacgaacat
     3541 tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa caaccttctc
     3601 aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgcttaggct gggcgttact
     3661 gaacgttttc gtcaaccttt tggggtcttt tgccatgacc gcactggctt tctggctgtt
     3721 ttccgcctca accgcacact aaagaccaaa aaaaacccgc tgattaagcg ggttttgaat
     3781 tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt tggcaccgtt
     3841 agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat tagtctggat
     3901 tgcagagaag tgtacaaaca cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc
     3961 tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa ttacctttac
     4021 atgaaaaata gacacaaatg ctgtgtcggt taccagtaca ccaattgtgg tacgctttgt
     4081 ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt aattatttta
     4141 cccattgatt ttaaagagtt tactaaaatt tcatttgtct caaaactgaa agcgcatcca
     4201 ggcaaagtac acattgccat tgttataggt acccggaatg taggtcatct gaaaagtcac
     4261 tggaccataa cccacagagg ccaatggcag tagaaccggg agagggatgt aattccagtt
     4321 atcgcgtgcc gttacgccag cggtgaatcc cagtcctaaa tgaaaatttt catccgccag
     4381 cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt tccacgagtc
     4441 cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac gcgactgacc
     4501 aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa
     4561 acgagcatgc caggtgatgg caggaatata taaatcataa tgttcaggct gttgccaggt
     4621 ttgtgtaata ttttctccaa acgttgtcat ccactcatct gcgttagcaa aaactttacc
     4681 aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac tcacgttcat
     4741 ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct acaattaaca
     4801 taaccttaat agccccaaca taaagaacaa tctgaatagc tgctctattt atttccgctt
     4861 gtttaagtta aatatttccg cgcatcgatt aaagatgatg tatcgttttc tgcgatggga
     4921 atagtcaaaa aagaaaaacc aagtattttt tgatgacaaa atgcaaccaa ggaaaaattt
     4981 ctttattttt taattggata caaaaatcac cttaacgaag aatataatat atatcgtcaa
     5041 cccatgctca tggcagatta ttttctgagc acgcaaccgc agctaacacc attgctatta
     5101 acaaatttgc atcaatccgc catcaattta cacacattat tatgtggtaa ttgccaaacg
     5161 ctaaatatgc gttttgttat ctatgtataa aaacagcaac ttcaatgtct taatggcagt
     5221 ttttcttgat tttaatcagc attcaccgcc aatttattgg gcatattttt tccttaagtt
     5281 ttaggaattt tttattattt actttggggc ctggagacag gaaaaattat gctgacattc
     5341 attgaactcc ttattggggt tgtggttatt gtgggtgtag ctcgctacat cattaaaggg
     5401 tattccgcca ctggcgtgtt atttgtcggt ggcctgttat tgctgattat cagtgccatt
     5461 atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc cacggatatc
     5521 gttgaatacg ttaaaatatt gctaatgagc cgcggcggcg acctcggcat gatgattatg
     5581 atgctgtgtg gctttgccgc ttacatgacc catatcggcg cgaatgatat ggtggtcaag
     5641 ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat tgccgcctat
     5701 tttgtcgcct gtctgatgtc tctggccgtc tcttccgcga ccggtctggg tgttttgctg
     5761 atggcaaccc tgtttccggt gatggtaaac gttggtatca gccgtggtgc agctgctgct
     5821 atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt ggtgctggcg
     5881 gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct gcctatctca
     5941 attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta tctggataaa
     6001 aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac tgctcctgcg
     6061 ttttatgcca ttttgccgtt cacgccgatc atcggagtgc tgatttttga cggcaaatgg
     6121 ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc ctccattctg
     6181 gagttcatcc gcagctttaa tacccagaaa gttttctctg gtctggaagt ggcttatcgc
     6241 gggatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg ggtattcgct
     6301 caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgccac ctcgtttggt
     6361 tcggcgagta tcatcctgat gctggtatta gtgatcctga caatgctggc ggcagtcacg
     6421 accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa actggcgcac
     6481 tcttccggca ttaacccggc gtatttgact atcccaatgc tgcaggcgtc aaacctcggt
     6541 cgtaccctgt cacccgtttc tggcgtagtc gttgcggttg ccgggatggc gaaaatctca
     6601 ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg ttgggctggt gattgttatc
     6661 gttgctacag agctgatggt gccaggaacg gcagccgcgg tcacaggcaa gtaaatagtc
     6721 atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat atgcgttgcg
     6781 gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg gcgcaatatt
     6841 caagataacg tctggcagtg gtgcggctga tagtcagtgc ctgcgccacc gtttctgccg
     6901 tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc aaggggtcaa
     6961 tgccgatcgg cagttcgtct ttgggttcac cgcgagcata ggcattaaac atctcatcaa
     7021 tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg cggaaacggg
     7081 ttagcgtttg ccccagccgt tcataggcaa ttggcttaat gagataatca aatacaccac
     7141 aacgtacggc ctcagacacc gtttccatat cgctggctgc agtggtaaac accacgtcgc
     7201 cgggataatg cgcctgcacc agttcatgca gtaaattaat ccctctaccg tcaggaagat
     7261 agttatcgag caagattagc cccggcttaa aacgctcgat catcattcgg gcctgcgcca
     7321 ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg acgaatatat tccgcatgca
     7381 tctctgccag cggcgtttcg tcctcaacga tcaatagggt taatggagct gtcattaggt
     7441 ttcactttcg gaatatagat tgaaaataag gtaccgcagg gatcattatc ttcgagagtg
     7501 ataacaccac cgcagcgcgt tacgtaactg gcaatcaagt acaacccaat gccatgttcg
     7561 ccgggctcgt cagcacgcgt actgacaccc tgctcaaata ttttgtctcg tagagactct
     7621 ggaacgccgc agccctgatc ggcgacttca atcaccacat catcgccttc atcgctgagg
     7681 aataattcaa cgatcttgtt tccttcatcg ctacgcaggc tggcttcgaa ggcgttatca
     7741 agtaaattgc caacaatggc tgcaaactcg gtgctgtcca gtcctggcgg cagttgcgaa
     7801 agctgacttc cggggacgat gatcattttt agccccagtt cgcgggcgcg ctgcacttta
     7861 ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct cgcgcaggct atcaataagc
     7921 tgttgctggg cctgagactc cccctgcacc atcgccaaca cgcgatcata ctctttcatc
     7981 tgcaacagac cattgagcgt cgacatccaa ttgagatgct cgtgtcgcaa tgtgcgcagg
     8041 ctctcgacgt attgtttaat ttgcgtcagt tgcgcattga gggtggatat ttcgtcttta
     8101 ctacgaaagc tgataatggc ccccagcaaa tcatcacctg aacgaatagc ttcccggttg
     8161 gcaataacgc tcagaccgtt aaagttcgcc accacatcct gacgtttttc atcaatctgt
     8221 tcggtaaaga aatcggcggg cctgaccact tcagcaatgg gtttacccaa ccattgccgt
     8281 ccgggggaac tcagacccag catctttctt gcgttacgat tgatggcggt aatgtaacca
     8341 tgcggatcca ccgcaatgag cccttcataa accgaactaa acagcgcttc ttgctgacgg
     8401 accacgcgtg cgatttgctt tggctccatg cccatcatct gccgacggat atgcgcggcc
     8461 aggaaccacg atagcaacat cagaatccct aacagcacga caaacacacc tgccatcggt
     8521 aataaaaact cagcacgcca gctatcgatt ttactcacca ggtagccaat cgacaccacg
     8581 ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg cacgcatcgc catgccaatt
     8641 gaccctttcc cggtgatgaa gtagctctcc cctttctcca gcgcgcccgg tttggtgaac
     8701 tgcataggat aaccaatttt ctccggatta ggatggtaaa gacgaatcga gtgccgatcg
     8761 ccaatcacca cataatcgaa atcagtatct ctttgtaatt tgtcagcgat ggtcgccagc
     8821 ctcttgtagt cacgcgtttt cactgcgctg atgatactgt cattagaagc aataattttt
     8881 gcctgattca ttgccatgtc gcgcacatga agcgttaaat agtcctcaaa actggccgta
     8941 aaatattgcg ccagggctgc aatgacaaat attgagaaca ccagaatcag caaaaagata
     9001 cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt tattctcgtt aagctgcaac
     9061 attgttttcc ttgcgggttg tcccgagcct ctttgacgga tgtaattaaa atgttgaatc
     9121 aaaatacata gatattaaat ataatcgtgg caaattttat tcattaaatc aattaaatca
     9181 cttaaacctg cgggtcattt cgtaattaca ttaattaaaa aacttaaaac cattaaataa
     9241 ataaaaaacc actaactcta tgtgaaataa atcaaaattt cacgctgcaa tactccttag
     9301 gatgtatagc gaaaggagaa aaagatatac ctcgatcgcc cccctttctc ccaagtgaaa
     9361 ataaaaggtt atcagtttgc aacatcgaac aacattcgtt gcaaatcgat aacaacatgc
     9421 accttcagga tactatttat tatgttcggc aatgatattt gcacccgtgt aaaacgttca
     9481 gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac
     9541 accacagtcg aagtatttat taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga
     9601 attgccggaa atattattaa atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg
     9661 gcgctgacat tagccaccga gctaattaac ctcgcctatg agcggcacag cacgcatctg
     9721 tttatttata ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt ttccacgctg
     9781 accagtgtac ccggcgtgat ggtgctgatg gaaaacagcg ccacgcgact gaaacgctat
     9841 gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac
     9901 gccaatccct ttacgaatgg tcaccgttat ctgattcaac aggctgcagc acagtgcgac
     9961 tggttgcatc tgtttttagt taaagaagat tcttcacgct ttccctatga agaccggctg
    10021 gatctggtgt taaaaggcac cgccgatatt ccacgcctga ctgtgcatcg cggctccgaa
    10081 tacatcatct cccgcgctac gttcccttgc tacttcatta aagaacagag cgtcattaac
    10141 cattgttaca ccgaaattga tctgaagatt ttccgtcagt acctcgctcc cgcgctgggt
    10201 gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag
    10261 gatatgcgct actggctgga aacgccgact atctccgcac cgcccatcga actggttgaa
    10321 attgagcggc tgcgttacca ggagatgccg atatccgctt cccgggtacg tcaactgctg
    10381 gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg cagtcacgct gcattatttg
    10441 cagaacctgc ttgagcactc ccgccaggac gcggcagctc gtcaaaagac ccccgcatga
    10501 gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt gagtctgggg
    10561 atgtgatgat acgcatcgcc ccactcgata cgcaggatat cgacctgcaa atcaacagca
    10621 gcgttgagaa acagtttggc gatgcaattc gcaccaccat tctggacgtt ctcgcccgct
    10681 acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg cgcactggac tgcattttac
    10741 gtgcacgact ggaagccctg ctggcacgcg ccagcggtat cccggctctg ccatgggagg
    10801 attgccaatg atttccgctt cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt
    10861 gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc cggctgatgc
    10921 cctgatgttt gacctcgaag actccgtggc attacgtgaa aaagacaccg cccgccgcat
    10981 ggtttaccac gcgctgcaac atccgctgta tcgcgatatt gaaaccattg tgcgtgtaaa
    11041 cgcgctggat tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga
    11101 cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt ctggatatcg aaaaagagat
    11161 cctgcgtatc gaaaaagcct gtggtcgtga acccggcagt accggcttgc tggcggcgat
    11221 tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat
    11281 cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg cgtacagaac gctccccgga
    11341 aggaattgaa ctgctgttcg ctcgctgctc cattttgcag gccgcgcgct ctgcgggtat
    11401 tcaggcgttc gataccgtct attccgacgc taacaacgaa gccggattcc tgcaagaagc
    11461 cgcccacatc aaacagctgg gctttgacgg caaatcgctg attaacccgc gtcagattga
    11521 tctgctgcac aacctctacg caccgaccca gaaagaagtg gatcacgccc gccgcgtcgt
    11581 agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga atggcaagat
    11641 ggtggacggt ccggttatcg atcgcgcccg tctggtgctc tcccgcgcag aactttccgg
    11701 catccgcgaa gaataaggca atcaaaatga cgcagaaaat tgaacaatct caacgacaag
    11761 aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct tgccgctttc cagaactcgc
    11821 caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa actgtgcgcc aacctggaag
    11881 aagcgattcg tcgctcgggt ttacaggacg gtatgacggt ttccttccat cacgctttcc
    11941 gtggcggtga cctgaccgtc aatatggtga tggacgtcat cgcgaagatg ggctttaaaa
    12001 acctgactct ggcctccagc tccctgagtg attgccatgc gccgctggta gaacacattc
    12061 gccagggcgt ggttacccgc atttatacct ctggcctgcg cggtccactg gcggaagaaa
    12121 tctcccgtgg tctgctggcg gaaccggtgc aaatccactc tcacggcgga cgtgtgcatc
    12181 tggtacagag cggcgaactg aatatagacg tggctttcct cggcgtcccg tcctgtgatg
    12241 aattcggtaa tgccaacggc tacaccggta aagcctgctg cggctccctc ggctatgcaa
    12301 tggttgatgc agacaacgcg aaacaggttg tgatgctcac tgaagaactg ctgccttatc
    12361 ctcataatcc ggcaagcatt gagcaagatc aggttgatct gatcgtcaaa gttgatcgcg
    12421 ttggcgatgc cgcgaaaatc ggcgctggcg cgacccgtat gaccactaac ccgcgcgaac
    12481 tgcttattgc ccgtagcgct gcggatgtga ttttcaactc aggctacttt aaagaaggtt
    12541 tctccatgca aaccggcacc ggcggcgcat cgctggcggt aacccgtttc ctggaagaca
    12601 aaatgcgtag ccgcgatatt cgcgccgact tcgcccttgg cggtattacc gcaacgatgg
    12661 ttgatctgca cgaaaaaggt ctgatccgca aactgctgga tgtacagagc tttgacagcc
    12721 acgctgcaca atcgctggcc cgtaacccca atcacatcga aatcagcgcc aaccagtacg
    12781 ctaactgggg ttcaaaaggt gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc
    12841 tggaaattga cacccagttc aacgttaacg tgctgaccgg ctctgacggc gtactgcgtg
    12901 gcgcttccgg tggtcactgc gataccgcga ttgcctctgc actttccatc atcgtcgcac
    12961 cgctggtacg cggtcgtatt ccgactctgg tggataacgt gctgacctgc atcaccccgg
    13021 gctccagtgt cgatattctg gtcacagacc acggtatcgc agttaacccg gcacgtccgg
    13081 aactggcaga acgtctgcag gaagcgggga tcaaagtggt ttctattgag tggctgcgtg
    13141 aacgtgcgcg tctgctgacc ggtgaaccac agccgattga attcaccgac cgcgtcgttg
    13201 ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt gcatcaggtg aaggaataag
    13261 ccatgcacct gcttcctgaa ctcgccagcc accatgcggt gtcaattccc gagctgctcg
    13321 tcagccggga tgaaaggcaa gcacggcaac acgtctggct caagcgccat cctgttccac
    13381 tggtctcctt taccgtggtt gcgcctgggc cgattaaaga cagcgaggtc acacgccgaa
    13441 tttttaatca tggcgtgaca gccttgcgtg ccttagccgc aaaacagggc tggcaaattc
    13501 aggagcaggc tgcactggtt tccgccagcg ggccggaggg catgttgagc attgccgccc
    13561 cggctcgcga cctcaagctc gccaccattg agcttgaaca tagtcatcct ctcgggcggt
    13621 tatgggatat cgatgtcctg acacccgaag gcgaaattct ctcccgccgc gactattcac
    13681 tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc tgtttgcgcg cgtggaaaaa
    13741 cccatcaact gaccgattta ctcaaccgca tggaggcgct gctgaacgat gtcgatgcct
    13801 gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa tcgatgagta cgccctgctg
    13861 ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga aaccaggcct cgtggatcgg
    13921 attaactgcg gtgcgcataa agatatggcg ctggaagatt ttcaccgcag cgcgctggcg
    13981 attcagggct ggttaccccg tttcattgaa tttggtgcct gtagtgctga aatggcacca
    14041 gaagcggtac tccacggatt acgcccaatt ggtatggctt gcgaaggtga tatgttccgc
    14101 gccactgcgg gcgtaaacac gcataagggc agcatttttt ctttagggct actgtgtgcg
    14161 gcaattggcc gtttgcttca gctcaaccag tcgctaacgc caacaaccgt ttgttctacg
    14221 gcggcaagtt tctgccgtgg cctgaccgat cgcgaactgc gtaccaataa ttcacaactg
    14281 acggcaggtc aacggttgta ccagcagctt ggccttaccg gcgcacgcgg tgaagccgaa
    14341 gcgggttatc cactggtgat caaccacgcc ctgccgcatt acctcactct gctggatcag
    14401 gggttagatc ctgaactggc attgctcgat accttgctcc tactgatggc gatcaacggc
    14461 gataccaacg ttgcatcgcg cggtggcgag gggggcctgc gctggctaca gcgcgaggcg
    14521 caaacattat tgcaaaaagg gggcattcga acccccgccg atctcgatta tctccggcag
    14581 ttcgacaggg agtgtatcga acgaaatctc agtccaggcg gcagtgccga cctgctgatc
    14641 cttacttggt ttttagcaca gatttaatta tttaagcact tgataaaatt ggaatattaa
    14701 ttgtcggaga acccgtatgt ctttagcaaa agataatata tggaaattat tggcgcccct
    14761 ggtggtgatg ggtgtcatgt ttcttatccc tgtacctgac ggtatgccgc cgcaggcgtg
    14821 gcattacttt gcagtgtttg tggcaatgat tgtcggcatg atcctcgagc caattccggc
    14881 aacggcgatc agttttattg cggttactat ttgcgttatt ggcagtaatt acctgctctt
    14941 tgatgccaaa gaattagctg acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg
    15001 tctggctggt ttttccagca ccacggtatg gctggtattt ggcgcattta tttttgcatt
    15061 agggtatgaa gtttccgggt taggtcgtcg cattgccctt ttcctggtga aattcatggg
    15121 caaacgcacg ctgacgttgg gttatgcgat tgtcattatc gacattctgc tggcaccgtt
    15181 tacaccgtcc aacaccgcgc gtaccggggg tacggttttt ccggtcatta aaaacctgcc
    15241 gccgctgttt aaatcattcc cgaacgatcc gtccgcgcgt cgtattggcg gctatttgat
    15301 gtggatgatg gtcattagta ccagtctgag ttcgtccatg tttgtcaccg gtgcggcacc
    15361 aaacgtactg ggtctggagt tcgtcagcaa aattgccggt atccagatta gctggttgca
    15421 gtggttcctc tgcttcctgc cggttggggt tatcttgctt atcattgcgc cgtggctttc
    15481 ctacgtgctg tacaaaccgg aaatcacaca cagtgaagaa gtggcaacct gggcgggtga
    15541 tgaactaaaa accatgggtg cgctgacacg cagagagtgg acgctgattg gtcttgtatt
    15601 gctcagctta ggtttgtggg tatttggcag tgaagtcatt aatgctactg cggtaggtct
    15661 gctggcagtt tcgctaatgc tggccctgca cgttgtgccg tggaaagaca ttacccgcta
    15721 taacagcgca tggaacacac tggtcaacct ggcaactctg gttgtgatgg ctaatggcct
    15781 gactcgttct ggttttattg actggttcgc cggtaccatg agtacgcacc tggaaggatt
    15841 ctcaccaaac gcaacggtga ttgtactggt tctggtgttc tactttgcgc actacctgtt
    15901 tgccagcctg tctgcgcaca ccgcaaccat gctgccggtt attctggccg tcggtaaagg
    15961 tattccgggc gtaccaatgg aacaactgtg tatcctgctg gtgctgtcta tcggtatcat
    16021 gggctgtctg acgccgtatg caaccggtcc tggggtgatt atttacggct gtggctatgt
    16081 gaaatcaaaa gattactggc gacttggtgc aatcttcggg gtgatttaca tctctatgct
    16141 gctgttggtt ggctggccga ttctcgccat gtggaactaa tacgttcgcc aaacgctgaa
    16201 taaatcatca cgcccgccag gtaagcccac ctggcgggct tttttatgat ttaatagata
    16261 gtattcagtg tatgtatgag ttccacaccc attatgaaag cattctggcg taacgccgcg
    16321 ttgctcgcgg tttctctgct tcccttctct tctgccaacg ccgtggcgtt gcaggcaaaa
    16381 cagtatggcg attttgatcg ctatgtcctg gccctctcct ggcaaaccgg attttgccag
    16441 agtcaacacg atcgaaatcg taacgaacga gatgaatgtc gcctgcaaac cgaaacgacc
    16501 aacaaagctg attttctgac cgtacatggt ctatggccag gattgcctaa atcggttgct
    16561 gcccgtggtg ttgatgaacg ccgctggatg cgcttcggtt gcgctactcg cccaatcccg
    16621 aatctaccag aagcgcgcgc cagccgaatg tgttcatcgc cggaaaccgg gttatcgctg
    16681 gaaacggccg ctaaactaag tgaagtcatg ccaggagctg gcggacgttc ctgcctggaa
    16741 cgctacgaat atgccaaaca cggtgcctgc tttggttttg atccagacgc atacttcggc
    16801 acgatggtac gcctgaatca agaaattaaa gagagcgaag ccggaaaatt ccttgcggat
    16861 aactacggta acacagtgag ccgccgtgac tttgatgccg cctttgccaa aagctgggga
    16921 aaagagaacg tgaaagcagt taagctaacg tgccagggta accctgcgta tttgactgaa
    16981 attcagatct cgatcaaagc tgacgccatc aacgctccgc tttctgccaa ctcattcttg
    17041 ccacaaccac acccaggcaa ctgtggcaaa acctttgtga ttgataaagc aggttattaa
    17101 tcctctgatt tacaaaacac gctaattcgt cttccataaa ccatgacgat taagatttat
    17161 acataatgcg ctacattgag gaattccctg aatgtgacgc aaatcacttc aaagagtgag
    17221 caacagtcag tatcatcatg tgagttaaac cctcgccgcc tgacggtgag ggttttcttt
    17281 tgggtatgtt tatctgcgac actcgcagta ccgacgacat ggagtaaaaa tgtccagacc
    17341 aactatcatc attaacgacc tggatgccga acgcatcgat attctgctgg agcaacccgc
    17401 ctatgctggt ttgccaatcg ccgacgcgtt aaacgcagag ttggatcgcg cccaaatgtg
    17461 ttcgccagaa gagatgccac acgacgtggt gacaatgaac agccgggtta aattccgcaa
    17521 tcttagcgat ggcgaagtgc gtgtgcgcac gctggtgtat ccggcaaaaa tgaccgacag
    17581 caatactcag ctttccgtta tggctccggt aggtgccgca ctgctggggc tgcgcgttgg
    17641 cgattccatt cactgggaac ttccgggcgg cgttgcaacc caccttgaag tgctggaact
    17701 cgaataccag ccagaagctg ctggcgacta cctgctttaa tcccatctgc acaagcgata
    17761 actcgctttg cccagaggat gcactcgcat cctcttttcg ctcaaatatc aaccagattt
    17821 atcattttgc tcttaagcgc agcattctaa tctgaactgg ttattcatct gcggaacctc
    17881 atatgagcga agctctcggt atcactgcat tttatatatt catagccgcg attattgttg
    17941 ctgcagtgct ataccttgaa caacgctggt agcgcctggt tttatttatt ttccagctca
    18001 atagcaaatt cagatcaaat cagcctcgca aatcatagat tcataaccaa taatgtgctg
    18061 ttatgtatac atgcaaaaac aaggagagaa tgcttatgta caagacaatc atcatgccag
    18121 tggatgtttt cgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc
    18181 aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc ctgagcctgc
    18241 accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa gcagaagaac
    18301 gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaag caacatgtcc
    18361 gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg gctgatgttg
    18421 tagttatcgg ttctcgcaac ccatcgattt cgacccatct gttaggttct aacgcctcga
    18481 gcgtgatccg ccacgccaat ctgccggtgc tggttgtgcg ttaattgcca gaataaatat
    18541 cccgccctgc ccggagccat cccggcgggg ctttttattg ccataaaaaa gccgccaggt
    18601 ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc tgttttggtg
    18661 cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt ggcgatgatg
    18721 atctgcttgt acggaaccgt tgtacagtca cccgctgcga acacgccttt cacgttggtt
    18781 tcgcatttcg catcaatgat aatctcgccc atgcggttac gttcgactgc gccttcgagc
    18841 cagttggtgt tcggcagcag accaatctgc acgaaaatac cggccagttc gatgttgtga
    18901 atatcgccgc tgacacgatc gcgatattcc agaccaacga ctttgctgcc gtcgcctttc
    18961 acttccgtgg tttgcgcatt cagaataatt tcgacgtttt tcaggctgcg cagtttgtcc
    19021 tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac gtgctcaacg
    19081 atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat aaccgctacg
    19141 cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga
    19201 tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc cacgataatg
    19261 ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcaggcc accttcaacc
    19321 gctgccggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc gtattcatca
    19381 acgtgaactt tcagcgcacc tgccagtttc tggccttcgg tcttcggtac agaaatgtag
    19441 ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat cagaccggta
    19501 cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc ggaaccgacg
    19561 attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt ttccgcgcca
    19621 gtatcaattt tggcgacgat ttcagtcaac gtcatgcgac cctgaccaaa ctctttcccg
    19681 tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg
    19741 ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag attcagcgcc
    19801 tgcaccacat ccgggcagtt gtggcaagag agcgagtaat aggtttcgaa ttcaaaatca
    19861 ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg atgaccaccg
    19921 gtccacaaca acgccagtac cagcgaggta aactcgtggc ccagcgggga acctgcaaaa
    19981 cgtggcccct ggttggaacc tgggttagtg atcaggaaag acggcttacg caccggcaag
    20041 ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc caacagttcc
    20101 ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc
    20161 aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat gtttatctcc
    20221 tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca gcttgcatca
    20281 tggtgcgggg aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa tttccgaagt
    20341 atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat ttagatttta ccaaccaggt
    20401 ccagagacgg agccagagtt gcttcacctt ctttccattt agccgggcaa acttcacctg
    20461 ggtgagaagc tacgtactgt gctgctttga ttttacgcag caggtcagac gcgtcacggc
    20521 caatgccttc agcggtaact tcgattgcct ggatgatacc ctgcgggtca acaacgaagg
    20581 tcgcacggtc agccagacct tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc
    20641 cagtcgggtc gccgatcatc gcatatttga ttttagcgat ggtttcagag ctgctgtgcc
    20701 atgctttgtg ggtgaagtga gtatcggtag atactgcgta tacgtctacg cccagtttct
    20761 gcagttcttc gtagtggtca gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt
    20821 cagccgggta gaagaagaag acgctccagc ggccttcggt atctttttcg gtgatttcga
    20881 tgaattcgcc gtttttgaat gcctggtttt taaaaggttt aattttggtg ttaatcaagg
    20941 acatctatac ttcctccgtg ttttcgatga gatgtaaggt aaccaatttt tgccgattcg
    21001 gctaatgcgt ttccattatc aaatcgataa gttttacctt acaaccttcg taagacaact
    21061 ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc
    21121 gtgtaatctg aactaccctt tggtatcttc agcgcctgcc tgcaggctga ggtgttgccc
    21181 ttacaaatgc aacaacgaca tggattacaa caccctcata aacaaagggc aatcacctga
    21241 tctaagctct tacctatgac agtgataggt tatgcctttt acccgacttt tgcactgact
    21301 gaaaaggaca aattaatgtt aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc
    21361 ttcgcagagg aacttcctga tccagtaaaa gcgattgaaa aacagggcat tacaatcatc
    21421 aaaacgttcg atgctcccgg aggaatgaaa ggttatctcg gcaagtatca ggatatgggc
    21481 gtcaccatct acctgactcc agatggtaag cacgctatct ccggttacat gtacaacgag
    21541 aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa tttacgcacc agccgggcgc
    21601 gaaatgtggc aacggatgga acaatcccac tggctcctcg acggtaaaaa agacgcgccg
    21661 gtcattgtct acgtcttcgc cgatccgttc tgcccatatt gtaaacagtt ctggcagcag
    21721 gctcgcccgt gggttgattc tggcaaagtg caattaagaa cattgttggt tggcgttatc
    21781 aagccagaaa gcccggcgac agcagcggca attcttgcct ccaaagatcc cgcaaaaacc
    21841 tggcaagaat atgaagcctc tggtggcaag cttaagctaa acgtgcctgc aaacgtaagt
    21901 acagagcaaa tgaaagtgtt aagcgacaac gagaaactga tggacgatct gggggcaaat
    21961 gtcacaccag ctatctacta catgagtaag gaaaatacac tgcaacaggc cgtggggttg
    22021 cccgatcaga aaacgcttaa tatcattatg gggaataaat aaaagataat tcatggatgg
    22081 ttgaaatata tccctatttt tcaaccatcc taaaaaagca tgaattgctc gctctgccct
    22141 aagtggctgt gctattttag aaaaacttaa ttctgagtta agcaaaaaaa cataatacaa
    22201 ataaatacaa cagcaacatt attgaacaaa tg
//
LOCUS       DAYPHN010000072        21926 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000072 DAYPHN010000000
VERSION     DAYPHN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.072"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..105)
                     /gene="pspB"
                     /locus_tag="ACG0BW_002594"
     CDS             complement(<1..105)
                     /gene="pspB"
                     /locus_tag="ACG0BW_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="DNA-binding transcriptional regulator; acts
                     together with PspC to induce psp operon during infection
                     with phage, exposure to ethanol or osmotic shock; forms a
                     complex with PspA and C; PspC is required for PspAB
                     binding; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFK7259544.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSEL"
     gene            complement(159..827)
                     /gene="pspA"
                     /locus_tag="ACG0BW_002595"
     CDS             complement(159..827)
                     /gene="pspA"
                     /locus_tag="ACG0BW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFK7259545.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            994..1971
                     /gene="pspF"
                     /locus_tag="ACG0BW_002596"
     CDS             994..1971
                     /gene="pspF"
                     /locus_tag="ACG0BW_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFK7259546.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGN
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(2091..3356)
                     /gene="puuE"
                     /locus_tag="ACG0BW_002597"
     CDS             complement(2091..3356)
                     /gene="puuE"
                     /locus_tag="ACG0BW_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFK7259547.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALND"
     gene            complement(3394..4674)
                     /gene="puuB"
                     /locus_tag="ACG0BW_002598"
     CDS             complement(3394..4674)
                     /gene="puuB"
                     /locus_tag="ACG0BW_002598"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFK7259548.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(4676..6163)
                     /gene="puuC"
                     /locus_tag="ACG0BW_002599"
     CDS             complement(4676..6163)
                     /gene="puuC"
                     /locus_tag="ACG0BW_002599"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFK7259549.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKVGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(6313..6870)
                     /gene="puuR"
                     /locus_tag="ACG0BW_002600"
     CDS             complement(6313..6870)
                     /gene="puuR"
                     /locus_tag="ACG0BW_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFK7259550.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(6897..7661)
                     /gene="puuD"
                     /locus_tag="ACG0BW_002601"
     CDS             complement(6897..7661)
                     /gene="puuD"
                     /locus_tag="ACG0BW_002601"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFK7259551.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            7873..9291
                     /gene="puuA"
                     /locus_tag="ACG0BW_002602"
     CDS             7873..9291
                     /gene="puuA"
                     /locus_tag="ACG0BW_002602"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFK7259552.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            9440..9604
                     /locus_tag="ACG0BW_002603"
     CDS             9440..9604
                     /locus_tag="ACG0BW_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259553.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            9594..10979
                     /gene="puuP"
                     /locus_tag="ACG0BW_002604"
     CDS             9594..10979
                     /gene="puuP"
                     /locus_tag="ACG0BW_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFK7259554.1"
                     /translation="MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            11113..11358
                     /gene="ymjA"
                     /locus_tag="ACG0BW_002605"
     CDS             11113..11358
                     /gene="ymjA"
                     /locus_tag="ACG0BW_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFK7259555.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGISPVRIDEIAEFLNQWGNE"
     gene            11671..13314
                     /gene="sapA"
                     /locus_tag="ACG0BW_002606"
     CDS             11671..13314
                     /gene="sapA"
                     /locus_tag="ACG0BW_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFK7259556.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            13311..14276
                     /gene="sapB"
                     /locus_tag="ACG0BW_002607"
     CDS             13311..14276
                     /gene="sapB"
                     /locus_tag="ACG0BW_002607"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFK7259557.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            14263..15153
                     /gene="sapC"
                     /locus_tag="ACG0BW_002608"
     CDS             14263..15153
                     /gene="sapC"
                     /locus_tag="ACG0BW_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFK7259558.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            15153..16145
                     /gene="sapD"
                     /locus_tag="ACG0BW_002609"
     CDS             15153..16145
                     /gene="sapD"
                     /locus_tag="ACG0BW_002609"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFK7259559.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            16147..16953
                     /gene="sapF"
                     /locus_tag="ACG0BW_002610"
     CDS             16147..16953
                     /gene="sapF"
                     /locus_tag="ACG0BW_002610"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFK7259560.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            17021..17386
                     /gene="ycjD"
                     /locus_tag="ACG0BW_002611"
     CDS             17021..17386
                     /gene="ycjD"
                     /locus_tag="ACG0BW_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFK7259561.1"
                     /translation="MMDKIKSNARDLRRNLTSQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRLSPSPQPSP"
     gene            17770..18558
                     /gene="fabI"
                     /locus_tag="ACG0BW_002612"
     CDS             17770..18558
                     /gene="fabI"
                     /locus_tag="ACG0BW_002612"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFK7259562.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELAKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            18702..19829
                     /gene="yciW"
                     /locus_tag="ACG0BW_002613"
     CDS             18702..19829
                     /gene="yciW"
                     /locus_tag="ACG0BW_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFK7259563.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLSPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAICMDSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            19897..21831
                     /gene="rnb"
                     /locus_tag="ACG0BW_002614"
     CDS             19897..21831
                     /gene="rnb"
                     /locus_tag="ACG0BW_002614"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFK7259564.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         4882 a         5844 c         5778 g         5422 t
ORIGIN      
        1 caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg gtaaaacaaa
       61 cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagga ctccttagac
      121 gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc ttgcttcatt
      181 ttggctttta attgtgccaa ttgttcgctg attgcatcat cggctttcag ttcggcaaac
      241 tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc ttccatctgg
      301 tcaattcgac gttcgaaaga ttcgaaacga gccattgctt catccagttt gccactgtcc
      361 agctgacgac gcacatcgcg cgacgagttt gccgcctgat ggcgtaacat caatgcctgc
      421 tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt cttcatgcgt
      481 gccagcgtat cgtccaccag cgtcacttca tgttccaggc tcttaatcag atcggtcagt
      541 ttctgttttt caattaacgc cgcacgcgcc agatcttctc tctctttcag cagcgccagt
      601 tcggcttttt cctgccattc aacttcacgc gccgacgctt gttctatacg gcgagtcagc
      661 tgtttctttt ctgccagcgc acgcgccgaa gtagaacgta cttcaaccaa tgtatcttcc
      721 atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc taacagagcg
      781 ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat gttgtcctct
      841 tgatttctgc gttcaggatt gtcctgctga actattaata caatttgcgt gccaattttt
      901 tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc aggatgagtt
      961 agcgaattac actaacaagt agcgaatttc atcatggcag aatacaaaga taatttactc
     1021 ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc accgctggac
     1081 aaaccggtgc tcatcatcgg cgagcgcggc acaggtaaag agctgattgc cagccgtctg
     1141 cattatctct cctcccgttg gcaggggccg tttatttccc ttaactgcgc ggcgttaaat
     1201 gaaaatctgc tggattccga actgtttggt cacgaagcgg gcgcgtttac cggtgcgcaa
     1261 aaacgtcatc cagggagatt tgaacgtgcc gacggcggta cgctatttct tgatgaactc
     1321 gctacggcac cgatgatggt gcaggagaaa ttattgcgtg tgattgagta cggtgaactg
     1381 gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc ggttggtatg cgcgacgaat
     1441 gccgatctcc cggcgatggt caatgaaggt aattttcgcg ctgacctgct cgaccggctg
     1501 gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat tatgctgatg
     1561 gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt cccggggttt
     1621 acggagcgcg ccagagaaac attgctgaat tatcgctggc cgggaaatat tcgtgaattg
     1681 aaaaacgtgg tggaacgttc agtatatcgc cacggcacca gcgattatcc gcttgatgac
     1741 attattattg atccctttaa acggcgtccg cctgaagacg ctatcgccgt ttcagaaacc
     1801 acctcgcttc caacactgcc gctggattta cgtgagtttc agatgcagca ggaaaaagag
     1861 ttgctgcaac tcagtttgca acaagggaaa tataaccaga agcgcgcggc tgaattactg
     1921 gggttaacct atcatcagtt ccgcgcgttg ttgaaaaagc accagattta gcgaagttgg
     1981 gcaatacaac ttgccggatg cggcgtgaat gctttgtccg gcatacataa tcaatacgtc
     2041 gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta ttaatcgttc
     2101 agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat ggtcagcgga
     2161 tacaggaaac gaatcacgtt gccgtatgcg ccacaggtca gcagaagcag cccctgcgcc
     2221 agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt ttgcggatcg
     2281 ttaaactctg ccgcaatcat agaccccaga ccgcgtaccg ccgcgatggc cggaacgctt
     2341 tctttggcat caatcaacgt gtttgtcagg cgctggccca gttgattcgc gcgttcacag
     2401 agtgattctt tgtcgataat gttgagcaca gcgtgcgcgg cagccaccgc cagcgggttc
     2461 ccggcgtaag taccacccaa cccgcccggc gcgggcgcgt ccataatatt cgcgttaccg
     2521 accacgcccg aaagcggcat gccgcctgcg aggcttttcg ccatcgtcat taagtccggc
     2581 ttatcggcgt agtgatccat ggcaaacagt ttaccggtac gcgcaaagcc gctttgcact
     2641 tcatcggcaa tcatcacaat gccgtgctcg tcgcacaggc ggcgaatggc ggcaaccagc
     2701 tcttttggtg caacgttgaa accgccttcg ccctgcaccg gttcgaaaat aatcgccgcc
     2761 acctgcttcg cttcaatgtc cgatttaaac aagcgttcga tggcgtcgag agagtcctgc
     2821 gttgaaacgc catgtaaatc tgacggataa ggtacgtgat ataccgaacc ggggaacggg
     2881 ccgaagccga ttttgtacgg cgcgaccttt ccggtcagcg ccatggtcat atatgtacga
     2941 ccgtggaagc cgccgctaaa cgcaatcacg ccagggcgtc cggtatgggc gcgggcgatt
     3001 ttcaccgcgt tctcaaccgc ttccgcaccg gtggtgaaga acgcagtttt agcctgcccg
     3061 ctcaccgggg caagggcgtt aattttctcc gccagggtga cgtagctttc gtacggcaca
     3121 atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc gaccagatca
     3181 gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat gtattcgttg
     3241 ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt acacatcacg
     3301 ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt gctcatgttt
     3361 cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat cgcgcaggct
     3421 gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc cggggaacgg
     3481 gtaatgcggc agattggcga aggcatcgaa acgttcggcg tcgccgcgca gcagttcggc
     3541 aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac cctgcatgta
     3601 atagatgttg gtatcaaggc gaccgaactg cggcatgcgc gacaaggtca gcaggaagtt
     3661 gcccgtccag cggtaatcaa ttttcacgcc cttcagctgc gggaaggttt tcagcagttt
     3721 cggcaccaca aggcgctcaa cgtcatccgg gtcgcgcgcg ccgtagacca cgccgccgcc
     3781 gtacagcagg cggttatcgg cggtaagacg gtagtaatca agcagatagt tacagtcttc
     3841 aacgcagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc gctcggtggt
     3901 gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta ctttatcgcc
     3961 cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat tggcagtccg
     4021 caccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat acacgcgccc
     4081 gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt gaatatgccc
     4141 accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc ggcgaatagc
     4201 gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct ctttctgctc
     4261 ttccagagtc gcgagctgtt tatcattcat cgccacaaac aggccgccgg ggcggtaatc
     4321 gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccgcctt cgaacatcat
     4381 gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatgacgt cgatgtcgcg
     4441 gctgtaagag ttcacaagct gcccgccgtt gcgtccgctt gcgccgaagc cgatgcgtga
     4501 ggcttcaaga accaccacgt caaagcccgc ttccgccaga tgcagcgcgg aagacagccc
     4561 ggtatagccg ccaccgacca cgcaaacgtc gcaggaaatc gactcattca gcgtgtcgaa
     4621 tggcgcgtat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg tcatttcagg
     4681 cctccaggct tatccagata gttttcagtt cggtgaattt ttcaagggcg tgcagggatt
     4741 tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata tcgccgtcgt
     4801 tgtagttatt gacgaagacg gagccgactt tcaggcggcg actcatgcgg tgcgcgcggg
     4861 agaggtcgcg cgtccatact gccgcgccga ggccgtactg gctgtcgttg gcaagctgta
     4921 gcgcctgttc ttctgatgtg aaacgcgtga ccaccagcac cggaccgaaa atctcttcgc
     4981 ggcttaagga cgcattcggg tctacctcca caaagatggt cgggccgatg gcggcagcca
     5041 gctcggcgtt acggccatcc agcagcagtt gccctttgct ttcgccttcc cgaataaagc
     5101 tatggaccga gtcggcgtgg gcgcagtcga ttaaggtgcc catggtggtt gcgggatcaa
     5161 gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag aattcatcgg
     5221 cgatgctctc ttccagcaac aaacgcgttc cggcgatgca cacctgcccc tggttgtaga
     5281 aaatgcccgc agcggtcgcg ctcgccgctt tttgcaaatc cggacagtca gcgaaaacga
     5341 tgttggcgct tttgccgccc gcttccagcc agacgcgttt catgttgctg tcgcctgcat
     5401 ctttcagcag ctgtttcccg gtacgggtcg aaccggtaaa ggcgatggca tcgatatcgt
     5461 tatgacgcga cagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg ttcaacacgc
     5521 catccggcag acctgcttct ttcgccagcc cggcgagacg aatcgcactg agcggtgatt
     5581 tttcagacgg ttttaggatc acgctgtttc ccgccgccag cgccgggccg agtttccagc
     5641 aagtcagcag cagcgggaag ttccacggta cgatggcggc aatcacgccg accggttcac
     5701 gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac actttgtcga
     5761 tagcctcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca cgcaggctgt
     5821 gacgaatcgg tttgccggtg tcgagcgttt ccagcagcgc cagttcttca gcgttagctt
     5881 ccattaaatc ggcgagttta ttcagcaccg ctttacgctt agccggagaa gagagtgacc
     5941 agtcgccacg ttcaaacacg ccgcgtgctg cgctcaccgc acggtcaata tcgacgctct
     6001 tgccgcgggc aatattcgcc agcggtgcct gggtgaccgg atcaacggtt tcaaaagttt
     6061 cattttccgc cgcagcagta tattcaccgt taataaataa gcgggtttca atggcgagac
     6121 ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact cctgtttcac
     6181 gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg gccgtatgcc
     6241 cgtttatttc tggcgacatt ctttatagat tgccgggcgc attgcgctta cccggcttac
     6301 agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc aaatacctgc
     6361 cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt gccccgcgac
     6421 gagatgataa tcctgaccat taatcgtcag aacaatttca ccttccagta cagtgcctat
     6481 ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt aagtttcaaa
     6541 gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg acacaccctg
     6601 actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat caggtttttc
     6661 cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct tttgcagcgt
     6721 actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt gagtcagccc
     6781 ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt ccgacaagcg
     6841 ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg tagtggtcag
     6901 agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc gaacaatata
     6961 cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc cagcgcaaaa
     7021 ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc ttcaacacgc
     7081 aaccgtgggc taacgacctt cgccccttgt ccatgcagag agtttaccca aaagttgcta
     7141 cattcaggta acaacgcaga cagcaatccc ccctcttcaa cctgaacttc atgcgacggt
     7201 gcatattgct gctccaccgg cagttcggga tcttcccgat gttccagcaa ttcaggctgt
     7261 tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcctg taaaccccgg
     7321 cagatggcga aaatggggat gcgcctttcg agtgcggcat taattaacgc catgctcaga
     7381 agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag gtgcggctgc
     7441 acattgctgg gactaccagg aagataaata ccatcgagtt tcggcaaaag ttgttcaagt
     7501 aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc gtggatgatg
     7561 gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct gttcctgcac
     7621 attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct cacaaagtgg
     7681 actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt ttttcaatct
     7741 agcagtgggt tttcattttt gcaaactcaa tttaacattt gacaaacatt tagtttgcat
     7801 acagattcga atagtggtca ttatatttta cgctttgata acgagcggaa aacaaaccaa
     7861 aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca gcagtcagaa
     7921 gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg ctacccgaat
     7981 acgcaatatg ttgatgtttt acttaccgat ttgaatggtt gcttccgtgg aaaacgtatt
     8041 cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc ggtgtttgca
     8101 atggatattc tgggcaacgt agtagaagaa gcgggcctgg gtcaggaaat gggcgagccg
     8161 gatcgtacct gtgttcctgt tctcggttcc ttaacccctt ctgccgccga tccagagttt
     8221 atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga cgttgagccg
     8281 cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt cccggtcgta
     8341 gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg atatctgcaa
     8401 ccgccctgcg cgcccggcac cgatgaccgc aatacacaaa gccaggttta ctccgttgat
     8461 aacctcaacc acttcgccga cgtgctcaat gatattgatg agctggcgca gttacagctg
     8521 attccggcag atggcgcggt cgctgaggcc tcgccaggtc agtttgaaat caatctttac
     8581 cataccgata acgtgctgga agcttgcgat gatgcgctgg cactaaaacg cctggtgcgt
     8641 ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta tgaagagcac
     8701 gcgggcagcg ggatgcatat ccatatcagt atgcaaaata accgtggcga gaacgtgctt
     8761 tctgacgcgg aaggcgaaga ttcgccgctg ctgaaaaaga tgcttgccgg gatgattgac
     8821 ctgatgccgt catcaatggc gttgctggca ccaaacgtga actcgtatcg ccgcttccag
     8881 ccaggtatgt atgtaccgac gcaggcgtcg tggggccata acaaccgcac cgtcgccctg
     8941 cgtattccgt gcggcgatcg ccataatcac cgcgtggaat atcgcgtggc gggtgccgat
     9001 gccaacccgt atctggtgat ggcggcgatt tttgccggta ttttgcatgg ccttgataac
     9061 gaactgccat tacaggaaga agtcgaaggt aacgggctgg aacaggaagg cttacccttc
     9121 ccgattcgtc agagcgatgc cctgggtgag tttatcgaga atgatcacct gcgccgctat
     9181 ttaggcgaac ggttttgcca tgtctatcac gcctgtaaaa atgatgagct gttgcagttt
     9241 gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta ataccccttg
     9301 cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta atccagacat
     9361 tttccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac gattaagact
     9421 gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg gaggctgcgt
     9481 atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc taaacaggtt
     9541 tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca actatggcta
     9601 ttaattcacc actaactatt gctgcgcaat ctggcaaaac ccgtctgcga aaatcactga
     9661 aattgtggca ggtggtgatg atgggtctgg cctatctcac gccgatgacc gtatttgata
     9721 cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat ttgctggcgc
     9781 tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag tttccggaag
     9841 ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga tttatggtcg
     9901 gctggtcatc gctgctggat tatctctttt tgccaatgat caacgtttta ttggcgaaaa
     9961 tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta accttcgtcg
    10021 ccattttaac cgccgcgaat ctgaagagcg tcaacctggt tgctaacttc aataccctgt
    10081 ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt cagggactgc
    10141 ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc gagaacgcgc
    10201 acctgatccc gattattacc ggggcgacga ttgtctgttt ctccttcctc ggtttcgatg
    10261 cggtgaccac gctttcggaa gagacaccgg acgccgcacg cgtgatcccg aaagccatct
    10321 tcctgacggc ggtatacggc ggcgttatct ttatcgctgc gtcgttcttt atgcagctgt
    10381 tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa attgcgctgt
    10441 acgtcggcgg caagctcttc cagtcgattt tcctctgcac cacgtttgtg aacacgttag
    10501 cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg gggcgtgaca
    10561 atgtgtttcc ggagcgcgtg tttggctatg tgcacccgaa atggcggact ccggcactga
    10621 acgtcattat ggtcgggatt gttgcgctgt cggcgctgtt ctttgattta gtcaccgcga
    10681 cagcattgat taacttcggt gctctggtgg catttacctt cgtaaatctg tcggtgttta
    10741 atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc cactatttgc
    10801 tgatgccgct ggttggcgcg ttgacggtgg gtgtgctgtg ggttaacctc gagtcaacgt
    10861 cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg tatctgatcc
    10921 gccgctatcg caaagtgccg ttgtatgatg gtgacagaac gccggtgagc gaaacgtaat
    10981 agtcggatgc gcggcgcatt atcctgcctg agggtaatgc accgcgccat taactataaa
    11041 aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc gcattaaccc
    11101 caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc cgatgagtac
    11161 gcgaccgaat gcgcagagcc tgtcgcagac gctgaacgca caccgctggc ccactacttc
    11221 cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc ccaacatgaa
    11281 atggctgctg aagcggggat tagtcccgta cgcattgatg agatcgcaga gtttttgaat
    11341 caatggggta atgagtaatt atttcattgc agacgctcgg tcagtatcgt tgcccggata
    11401 aagcgttcac gccgcatccg gctaaaaatg acacgcactg cagcgtgctt taatgggttt
    11461 ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag ttatagagaa
    11521 atcacgggta cgaatgttga acagatggtg acgtcacctg ttcaacgacc aaccacacgg
    11581 ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat attagtaaga
    11641 tagcctcttt acataaaatc cattaatatt atgcgccagg tattatcgtc tcttttggtg
    11701 attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc gcatgctgat
    11761 atccgcgaca gcggttttgt ctattgcgtt agcgggcaag tcaacacctt taacccatcc
    11821 aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga tcgactgctg
    11881 gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg ggaagttctc
    11941 gataacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac
    12001 tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt
    12061 tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta cttcgacagc
    12121 ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac cgttgagttc
    12181 cgactcgccc agccggatgc ttcttttttg tggcacctgg cgacccatta tgcatcggtc
    12241 atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca actcgaccgt
    12301 caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca atttattcgc
    12361 ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat
    12421 ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg cgacgttctg
    12481 gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt gcgtttaacg
    12541 ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc gccgctaaat
    12601 aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct gatgcaatcc
    12661 atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc gtgggcctat
    12721 gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca gttgaaggcg
    12781 ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca ggcgtggaac
    12841 cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt tggcgtaaaa
    12901 gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga tatgagccat
    12961 gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt cttccgtcct
    13021 ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg cgatccgaaa
    13081 ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg tattgaagcc
    13141 tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct ggcgtcgtca
    13201 ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc gtttggtaac
    13261 gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc atgattatct
    13321 tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc tttgttggct
    13381 tcagcttgag ctatttcacc ccacatgcgc cgttacaagg cgcgtcactg tggaatgcct
    13441 gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc attaacggtc
    13501 aaccgattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg tgcatcctcg
    13561 ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg attacgcgcc
    13621 ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca atcccggtgt
    13681 tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg ttgcccgttt
    13741 caggacgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg ttgattgatg
    13801 cctggctttc ggactcacca tggcgggatg aaatggtcat gagcgcaatc cgccatatga
    13861 tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccga ctgatgcgta
    13921 ttagcactat cgaagtgtat gaccagaact atgttaaagc agcggcgacc cgcggtttgt
    13981 cccgctttac tattttgcgt cgccacgttc tgcataacgc gttacctccg gttattcctc
    14041 gcctgggctt acagttttct accatgctga cgctggcgat gatcaccgaa atggttttta
    14101 gctggccggg tttaggccgc tggttaatta acgccatccg ccagcaggat tacgcagcca
    14161 tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg atttctgata
    14221 ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta cgatagcgta
    14281 tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac
    14341 gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg tatttttggc
    14401 ggctggtttg cgccttacgg catcgatcag caatttctcg gttatcaatt attgccgccg
    14461 tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct cggacgcgat
    14521 gttttaagcc gtttactgag cggagctgcg ccaaccgtag gcggcgcatt tgtggtgacg
    14581 cttgccgcta cgatctgtgg cctggtactg ggcaccttcg ccggcgcgac gcacggccta
    14641 cgctcagcgg tgcttaacca tatactggat accttactgg cgatcccttc cctactgctg
    14701 gcaattatcg ttgtagcgtt tgccggaccg agtttgtctc acgccatgtt tgccgtctgg
    14761 ctggcgctgc tgcctcgtat ggtacgttcg atttacagca tggtgcatga cgaactggaa
    14821 aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat tctctggttt
    14881 gctgtgatgc caaacatcac cgccgggctg gtcaccgaaa tcacccgcgc actgtcgatg
    14941 gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcgcaact cccctcgcct
    15001 gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc gtggactgtc
    15061 atgctgccag gtgcggcaat tatgattagc gtgctgttgg ttaacctcct tggcgacggt
    15121 gtccgtcgtg caattattgc gggggtggaa taatgccatt actcgatatt cgtaacctga
    15181 ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgcgta agtatgacct
    15241 taaccgaagg tgaaatccgc ggtcttgttg gcgaatccgg ttccggcaag agtttgattg
    15301 cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac cgtatgcgtt
    15361 ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaagctg gttggtcaca
    15421 acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa cgagtgggtc
    15481 gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg cagcgttttg
    15541 gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa gatcacaaag
    15601 atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa gtgatgatag
    15661 ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca aactcaatgg
    15721 agccaacaac ccaggcgcaa atctttcgcc tcctgacgcg tctcaaccaa aacagcaata
    15781 ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg gataaaatta
    15841 acgtgcttta ctgcggtcaa acggtggaaa ccgcgccgag taaggagttg gtgacgatgc
    15901 cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc
    15961 cgcataaaag tcgcctcaat acgctgcccg gcgctatccc actgctggaa cagttaccga
    16021 ttgggtgtcg tctggggccg cgttgcccgt atgcacaacg agaatgcatt gtgacgccac
    16081 gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac atggagaaag
    16141 agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt ccgctaccgg
    16201 accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt
    16261 gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac gctggcaaaa
    16321 atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga tcatccgctg
    16381 cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca ggatccttcg
    16441 acatcgttga atccccgtca acgtatctcg caaatactgg attttccact gcgtctgaac
    16501 accgatctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg tatggttggg
    16561 ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccgggca aaaacagcgt
    16621 ctggggctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga tgaagccctc
    16681 gcctcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga gttacaggaa
    16741 aaacagggca tttcgtatat ttatgtcacc cagcatatcg gaatgatgaa gcatattagc
    16801 gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac cgccgatgtg
    16861 ctggcatcgc cgctgcatga actcaccaaa cggcttattg ccggtcattt tggcgaggca
    16921 ttgacggcgg atgcgtggcg taaagatcgt taattttctg aatcctcctt ccagattatt
    16981 cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata aaataaaatc
    17041 aaatgcgcgt gatttaagac gcaatctcac ttcgcaggaa cgaaagctct ggcgatatct
    17101 tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg ggagctacat
    17161 tctcgatttt gcttgctgct cggcgcgtgt agtagttgag ctggatggtg ggcagcatga
    17221 tttggcagtt gcctatgatt ccaggcgcac tagctggctt gagtcgcagg gctggaccgt
    17281 gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg agattattct
    17341 gcaggaactg aaccgcctgt caccctcacc ccaaccctct ccctgaaagg gcgagggggc
    17401 cgaccgagcc gaatagctgt tgtggtgaaa acatggaggc ggatgatgaa gaatattcgg
    17461 caaggtctga accaccattc actctcaccc cagccatcgc cctgaaaggg ttagggggct
    17521 gtataagcct gtttgttgct ggggtgacaa tatttgcaca atacggtccc ctcgcccctc
    17581 tggggagagg gttagggtga ggggaaaagc gcccaccctg cccgcaccct gctccggtcg
    17641 gacctggcaa ctatagccac tcacagccag gttgattata ataaccgttt atctgttcgt
    17701 actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg
    17761 attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa
    17821 ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc
    17881 acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct
    17941 gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat gttcgctgaa
    18001 ctggcgaaag tttggccgaa gtttgacggt ttcgtacact ctattggttt tgcacctggc
    18061 gatcagctgg atggtgacta tgttaatgcc gttacccgtg aaggcttcaa aattgctcac
    18121 gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg
    18181 ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac
    18241 gttatgggtc tggcaaaagc gtctctggaa gcaaacgtgc gctatatggc gaacgcgatg
    18301 ggtccggaag gtgtgcgtgt taacgccatc tctgcgggtc cgatccgtac tctggcggct
    18361 tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc
    18421 cgtaccgtta ccattgaaga tgtgggtaac tctgcggcat tcctgtgctc cgatctctct
    18481 gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac
    18541 gaactcgaac tgaaataatc gttctgttgg tcaagacggg cggcgtcctg ccgcccgtta
    18601 tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct
    18661 tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca
    18721 ccggcaaaag ccactggtat catgaaacgc aatccagtac tgcggaatat gacgttctgc
    18781 ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg
    18841 aagaaacgct gtcacccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggagt
    18901 tgttccctga ccagcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt
    18961 cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac tactattcgg
    19021 cgcgacttac gccgctcccc gggcctgatt cctccaggga aagtaatcat cggttagcac
    19081 aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc
    19141 gccagcatct gaatgacgtc ggtcttactg tctgggactg tgtgatcatt aatcaaatca
    19201 ttggttttat tggctttcag gcgcggacca ttgcgacatt tcaggcttat ctcgggcatc
    19261 cggtacgctg gttacccggt ctggagatac aaaactatgc cgacgcgtca ctgtttgctg
    19321 atgagtcaat acgctggcga agcagctatg aggtggaaaa actacctgaa gagcacacaa
    19381 aaagttcaac tgcagaactt tgccaactgg ccgaaacact ctctctccac cctatttcac
    19441 tttcccttct cgaaaagttg ctaaacagca cacgggtcaa tacacagccg gataatcagc
    19501 ttgcggcgtt gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc atctgtatgg
    19561 attcatcaaa tgaatataaa aaaatcagcg cccttctgcg caagggcgaa aatgaaatta
    19621 accaatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga
    19681 cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa cacgaaaaat
    19741 catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct
    19801 taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac
    19861 tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta
    19921 gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc
    19981 acagaaaaag gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg
    20041 ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggttatcca cagtgaaaaa
    20101 gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt
    20161 aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact cttaaaagac
    20221 gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggca
    20281 gttgccgaaa tgcgccgcca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca
    20341 caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct tgcgcgccat
    20401 aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt
    20461 cgtgaagatc tgaccgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg
    20521 gatgacgccc ttttcgctaa ggcgttgccg gatggcaaac ttcagctgat tgtggcgatt
    20581 gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc
    20641 gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac
    20701 gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc
    20761 tccgctgatg gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa
    20821 gcgaagctgg tgtatgacca ggtctctgac tggctggaaa ataccggtga ctggcagcct
    20881 gaaagtgaag caattgccga acaagtccgt ttgttagcgc aaatttgcca acgccgcggc
    20941 gagtggcgtc ataaccacgc actggtattt aaagatcgcc cggattaccg ctttattctc
    21001 ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caatcgtatc
    21061 gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc
    21121 ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca
    21181 gcgttgctga aaacccacgg tctgcatgtc gatgccgaag aagtgctcac gctggacggt
    21241 ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt
    21301 cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt
    21361 ctggaggcat acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac
    21421 cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga tgaaatcact
    21481 gtccaaatgg ccgagcgtcg ccgtctcaac cgaatggcag aacgtgatgt tggcgactgg
    21541 ttatacgcac gcttcctgaa agacaaagct gggactgaca cccgtttcgc ggcggaaatt
    21601 gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc tattgccttt
    21661 attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc
    21721 actgtacaaa ttaaaggtga aacggcttac aaagtgactg acgttattga cgtcaccatt
    21781 gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc
    21841 acggcccatt cctcatgaat gggccattta tttccccgct ccaccttcgt catatcccgg
    21901 cagattttta actgactttc gtttga
//
LOCUS       DAYPHN010000073        21822 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000073 DAYPHN010000000
VERSION     DAYPHN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.073"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(100..423)
                     /gene="ydbL"
                     /locus_tag="ACG0BW_002615"
     CDS             complement(100..423)
                     /gene="ydbL"
                     /locus_tag="ACG0BW_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFK7259565.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETLELVKDINEARNLSYQQLAKQNNVSTDEIAKLAGQKMVARAKPGEYVQGING
                     KWLQK"
     gene            complement(431..616)
                     /gene="ynbE"
                     /locus_tag="ACG0BW_002616"
     CDS             complement(431..616)
                     /gene="ynbE"
                     /locus_tag="ACG0BW_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFK7259566.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(613..3252)
                     /gene="ydbH"
                     /locus_tag="ACG0BW_002617"
     CDS             complement(613..3252)
                     /gene="ydbH"
                     /locus_tag="ACG0BW_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFK7259567.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLYKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3460..4449
                     /gene="ldhA"
                     /locus_tag="ACG0BW_002618"
     CDS             3460..4449
                     /gene="ldhA"
                     /locus_tag="ACG0BW_002618"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFK7259568.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4560..4982
                     /gene="hslJ"
                     /locus_tag="ACG0BW_002619"
     CDS             4560..4982
                     /gene="hslJ"
                     /locus_tag="ACG0BW_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFK7259569.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4979..5245)
                     /gene="ydbJ"
                     /locus_tag="ACG0BW_002620"
     CDS             complement(4979..5245)
                     /gene="ydbJ"
                     /locus_tag="ACG0BW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFK7259570.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5519..9043
                     /gene="nifJ"
                     /locus_tag="ACG0BW_002621"
     CDS             5519..9043
                     /gene="nifJ"
                     /locus_tag="ACG0BW_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFK7259571.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRILRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9405..10538
                     /gene="ompN"
                     /locus_tag="ACG0BW_002622"
     CDS             9405..10538
                     /gene="ompN"
                     /locus_tag="ACG0BW_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFK7259572.1"
                     /translation="MKSKVLAFLIPTLLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10679..11113
                     /gene="uspF"
                     /locus_tag="ACG0BW_002623"
     CDS             10679..11113
                     /gene="uspF"
                     /locus_tag="ACG0BW_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFK7259573.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11289..12224
                     /gene="ttcA"
                     /locus_tag="ACG0BW_002624"
     CDS             11289..12224
                     /gene="ttcA"
                     /locus_tag="ACG0BW_002624"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFK7259574.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12353..13726)
                     /gene="dbpA"
                     /locus_tag="ACG0BW_002625"
     CDS             complement(12353..13726)
                     /gene="dbpA"
                     /locus_tag="ACG0BW_002625"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFK7259575.1"
                     /translation="MTAFSTLNVLPPAQLTNLDELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(13756..13929)
                     /gene="ynaL"
                     /locus_tag="ACG0BW_002626"
     CDS             complement(13756..13929)
                     /gene="ynaL"
                     /locus_tag="ACG0BW_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFK7259576.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(14204..15187)
                     /gene="zntB"
                     /locus_tag="ACG0BW_002627"
     CDS             complement(14204..15187)
                     /gene="zntB"
                     /locus_tag="ACG0BW_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFK7259577.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(15201..15248)
                     /gene="ydaN"
                     /locus_tag="ACG0BW_002628"
     CDS             complement(15201..15248)
                     /gene="ydaN"
                     /locus_tag="ACG0BW_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFK7259578.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            15442..16674
                     /gene="dgcM"
                     /locus_tag="ACG0BW_002629"
     CDS             15442..16674
                     /gene="dgcM"
                     /locus_tag="ACG0BW_002629"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFK7259579.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(16695..17258)
                     /gene="smrA"
                     /locus_tag="ACG0BW_002630"
     CDS             complement(16695..17258)
                     /gene="smrA"
                     /locus_tag="ACG0BW_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFK7259580.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17588..18496)
                     /gene="abgR"
                     /locus_tag="ACG0BW_002631"
     CDS             complement(17588..18496)
                     /gene="abgR"
                     /locus_tag="ACG0BW_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7259581.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKHLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKATYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            18672..19982
                     /gene="abgA"
                     /locus_tag="ACG0BW_002632"
     CDS             18672..19982
                     /gene="abgA"
                     /locus_tag="ACG0BW_002632"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFK7259582.1"
                     /translation="MESLNQFINSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALEQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEERDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFERAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVPGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            19982..21427
                     /gene="abgB"
                     /locus_tag="ACG0BW_002633"
     CDS             19982..21427
                     /gene="abgB"
                     /locus_tag="ACG0BW_002633"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFK7259583.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNCEELAFAKQIQ
                     ATLTLNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTIAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            21464..>21822
                     /locus_tag="ACG0BW_002634"
     CDS             21464..>21822
                     /locus_tag="ACG0BW_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFK7259584.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEVLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMAS"
BASE COUNT         5572 a         5591 c         5285 g         5374 t
ORIGIN      
        1 tcaatagatg caaataaaaa tcgccttagt ccagaactta ttttccctta aatattcaca
       61 aaatggcgca acataatgaa tatggatatg gatatgaaat tatttttgca gccattttcc
      121 attaatcccc tgcacatatt ctcccggctt cgcgcgagca accatttttt gccctgccaa
      181 ttttgctatc tcatcagtgg atacattatt ttgctttgcc aattgttggt aactcagatt
      241 acgagcttca ttaatatctt ttactaattc cagcgtttca gcatctgttt tcagcgcaac
      301 cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa
      361 tgccattaca ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt
      421 catcatcgcc tcagaaaaga tcgctacgag tctcaagcag ctcttcgaca tctttgtctg
      481 ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag
      541 gtgcagcgac ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag
      601 ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg
      661 cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca
      721 cagcgtaaaa atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc
      781 aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaaatt
      841 aatatttgtc gatgaacggc taatttccat atagcgcaac cagttaattg ctgaacccgc
      901 agtcatattg tctttcacca ccgcatctgc ggtgtcttta tccagccgca aggtcattgg
      961 cccgctattt gccagccagc catctttcac tatccatttt tcgttgttta gccataacgg
     1021 caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag
     1081 ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa
     1141 ttgtttcatc agcacgttac cgcctaacac atcgacgcta acatcagtca acaacaatgg
     1201 ttcttcttct gtccacggat agcgcccttg caaatcagcc gtaatatttt tcgctgtcac
     1261 cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc
     1321 tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat
     1381 ccaggcacta ccgcctttca acacaccgtg ccctcccgcg cggaatcctt gttcaggcgc
     1441 agcagaaaat gcgacctgag catacagttc gccatcgcgt aagttcatct tccagtcggg
     1501 tggcaccagc ggctggaata cggtcagtga ttgtttaggc caccaggcgt taccgcgcag
     1561 acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc
     1621 gcctttatat aggaaatacg tcggatcgcg tccatcgacg ctaaatttta aggttgatgg
     1681 cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct
     1741 aaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg
     1801 cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag
     1861 cgtaatcgtg ctgtcatgcc actcaccttt tcctgcaaca tcccaggtgg cattcatcgg
     1921 tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag ggagaaaatc
     1981 attcgccagc ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa
     2041 aatggcctgc aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca
     2101 gcggatttca tcgatgtcca gcgaatcgat gactcttccc ttcgaacgta ataacgcgcc
     2161 tggctcaaag gccagcgttg ggtcagtcag acttccgctt agctgcgcag gtaagcgggc
     2221 atataaaatg agatccgcct gtttcgcttc accggtcagc tgcaaaggca gctggctgtt
     2281 atccatgctt aattttccag ggccaaaatt aagcaccgcg ttgcccttac ccgcttgccc
     2341 ctgggtcagc acactcagtc gtccgctgac cagagcgttc tcgagccctg cctgccagtt
     2401 gtcgactttg acacccagtc tgccactcaa aggaaaacct gcatacggcc agctccagcg
     2461 accatcgctt acagtcaatt gttggcgagt aatttgccac ggcagatcga gcaacggatc
     2521 gccattatcc cgtgccagca caatcaattg cccgctattt tcctgccagt ccagctcggc
     2581 atccaccagt gaaggctcct gcggcaagtt taacgtcgca gtagcatgac cacttacagg
     2641 aagtccatcc ggcactagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt
     2701 ttcaaacgcg acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg
     2761 aaatttaact ttttcgccct gataacgcag ttgctggata tcggaggtta atgcgagaga
     2821 gagttttccc tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt
     2881 gttaggcaac atggcctgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt
     2941 ctgcggcaat ttcgccagac aagcagaatc aagttctacc gtaccgacgt tcaataacca
     3001 gcggctggga tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag
     3061 ataacggaga tcggggatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc
     3121 aatacgtgtc ccgagcggta gccagatgcc cgccagcgtg ggaacccaca agccgagcgt
     3181 catcagcagc gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata
     3241 tttacccagc atgggtagtt aatatcctga tttagcgaaa aattaagcat tcaatacggg
     3301 tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga
     3361 tgaatttttc aatatcgcca tggctttcaa ttatatttga aattttgtaa aatattttta
     3421 gtagcttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc
     3481 acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg
     3541 gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg
     3601 gtatgtattt tcgtaaacga tgacggcagc cgcccggtgc tggaagagct gaaaaagcac
     3661 ggcgttaaat atatcgccct gcgctgtgcc ggttttaata acgtcgacct tgacgcggca
     3721 aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaagc cgttgctgaa
     3781 cacgccatcg gtatgatgat gacgctgaac cgccgtattc atcgcgcgta tcagcgtact
     3841 cgtgacgcca acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca
     3901 ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt
     3961 atgcgtctgc tggcattcga tccgtatcca agtgcggcgg cactggaact cggtgtggag
     4021 tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg
     4081 acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg
     4141 atgatcgtca ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg
     4201 aaaaatcaga aaattggttc gttgggtatg gacgtgtatg agaacgaacg cgatctgttc
     4261 tttgaagaca aatccaacga cgtgatccag gatgacgtat tccgtcgtct gtctgcctgc
     4321 cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag cagaagcact gaccagtatt
     4381 tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gcgaaacctg cccgaacgaa
     4441 ctggtttaat cttgccgctc ccctgcattc caggggagct tattcagata atccccaaag
     4501 acctttcatc ctctattctt aaaatagtcc tgagtcagaa actgtaattg agaaccacaa
     4561 tgaagaaagt agccgcgctc gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg
     4621 acaaaattgc tgtaacccca gaacagctac agcatcatcg ctttgtgctg gaaagcgtaa
     4681 acggtaagcc cgtgaccagc gataaaaatc cgccagaaat cagctttggt gaaaagatga
     4741 tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac
     4801 tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac
     4861 tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg accgcgaacc
     4921 agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat ttaatgaatt
     4981 aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg ccgttgggta
     5041 atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc gaaccgccga
     5101 tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaacct ggcgctacgt
     5161 gcgccgcagt cgcctgctga acaggttcac tactacacgc cgacaataat aaagcggcac
     5221 accctaccca aaacgctgct cgcatctttt cttcctctga tcttcaagcc aaacgacacc
     5281 gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc gttgccccgc
     5341 atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat tcattacgct
     5401 gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa ttgaaagata
     5461 actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat
     5521 gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca gtgaagttat
     5581 cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg catgggccgg
     5641 aaacggcttg aaaaacgtct ggggagacac accacgcgtg gttgaaatgc agtcggaagc
     5701 gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa catcgtttac
     5761 gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag gcgaactgac
     5821 gccatttgtc ctgcatgtag cggcacgtac cgttgccaca catgcactct ctatttttgg
     5881 cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt gtgcagcaaa
     5941 cgtccaggaa gcgcaagact ttgctcttat ttcgcacatc gcgacgctga agagtcgcgt
     6001 gccatttatt catttctttg atggtttccg cacgtcccac gagatcaata aaattgtccc
     6061 gctggccgat gacactattc ttgaactgat gccgcaggcc gaaattgatg ctcatcgcgc
     6121 ccgggcgctc aacccggaac atccggtgat ccgcggtacg tccgccaatc ctgacactta
     6181 tttccagtct cgcgaagcca ccaacccatg gtacaacgcg gtctatgacc atgttgaaca
     6241 ggcgatgaat gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg agtattacgg
     6301 gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca cctgtgaaga
     6361 agtggttgat gaattgctaa cccgtggcga aaaagtcggc gtgctgaaag ttcgcctgta
     6421 ccgtcccttc tccgccaaac acttgctaca agctctgccg ggatccgtac gcagcgtggc
     6481 ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc tggatgtgat
     6541 gaccgcactg gcagaagcct ttaataatgg cgagcgcgaa accctgcccc gtgtcattgg
     6601 tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg cggtatttgc
     6661 cgagctcaac gcggctaaac cgaaagcgcg ctttacggtt ggtatttacg atgatgtgac
     6721 caatctgtca ctgccgttgc cggaaaacac cctgccaaac tcggcgaaac ttgaagcctt
     6781 gttttatggc cttggtagtg atggcagcgt ttccgcgacc aaaaacaata tcaagattat
     6841 cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca aaaaggcggg
     6901 cggcctgacg gtttctcacc ttcgcgtgag cgagcagccg attcgttccg cttatctcat
     6961 ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat atcagatggc
     7021 tgaacgttta aaacctggcg gcattttcct gctcaacacg ccgtacagcg cagatgaagt
     7081 atggtcgcgc ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag cgcgcttcta
     7141 tgtgattaac gcggcgaaaa tcgcccgcga atgtggtctg gcggcccgta ttaataccgt
     7201 catgcagatg gcttttttcc atcttacgca aattctgcct ggcgatagcg ccctcgcaga
     7261 attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg tggaacgcaa
     7321 ctggcaggct ctggcgctgg cacgtgaatc cgtagaagaa gttccgctgc agccggttaa
     7381 tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt tcgtgaaaac
     7441 cgtaaccgcg gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg cgctgccgcc
     7501 agacggcacc tggccgatgg gcaccacgcg ctgggaaaaa cgcaatatcg ccgaagagat
     7561 ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgctg cttgcccaca
     7621 ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc ctgccagcct
     7681 gcattcgctg gatgtaaaat cgcgtgatat gcgcgggcag aaatatgtct tgcaggtggc
     7741 accggaagat tgcaccggct gtaacctgtg cgtcgaagtt tgcccggcga aagaccgtca
     7801 gaatccagag attaaagcca tcaatatgat gtcgcgcctg gaacatgtcg aagaagagaa
     7861 aatcaattac gatttcttcc tcaacctccc agaaatcgac cgtagcaaac tggaacgtat
     7921 tgatattcgt acatcgcagc tgattacacc tctgtttgaa tattcaggtg cttgctccgg
     7981 ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggcgacc ggatgttgat
     8041 cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta caccgtatac
     8101 taccgatgcc aacggtcgtg gaccggcatg ggcgaactcg ctgtttgaag ataacgccga
     8161 atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcatac tgcgtctgct
     8221 ggatcaattt gccgataaaa tcccggcgga attactgact gcgttgaaat cagacgccac
     8281 gccagaggtt cgtcgtgaac aggttgcagc tttacgccag caactcaacg atgttgccga
     8341 agcacatgaa ctgctacgtg acgcagatgc actggtagaa aaatcaatct ggctgattgg
     8401 tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat tgagtttgac
     8461 ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaataccg gtggtcaggc
     8521 gtcaaaagcg acaccgctgg gtgcagtaac taagtttggc gagcacggca aacgtaaagc
     8581 gcgtaaagat cttggcgtca gtatgatgat gtacggccat gtttatgtgg cgcagatttc
     8641 tctcggcgcg cagctgaacc agacggtgaa agcaattcag gaagcggaag cgtatccggg
     8701 accatcgctg atcatcgctt atagcccgtg tgaagagcat ggttacgatc tggctctcag
     8761 ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc gcttcgatcc
     8821 tcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagaagc
     8881 actggaagaa acgttacttc atgagcaacg tttccgtcgt ctgaattcgc agcagccaga
     8941 agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct atgacttcct
     9001 ggcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgcact ggataaggat
     9061 tatccaattc taaaaaaaag cccggacgac tgttcgggct tgtctcatta tttgttggat
     9121 aaacagtaac aagggtccaa cagaaaataa tctttgtgac aataaaggca tataacccgc
     9181 gaagaataac gcatcactat ttaaatgtat aatttttatt ttgtatatgt cgccaataat
     9241 atattcacca aacttatcat catttccgta gaattatttg attaaggttt tacttaaggc
     9301 gtaacaaatg atatttatcg gctaacggaa cttctccttt aggatgtttt cactccccaa
     9361 aatggggatg aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agcaaagtac
     9421 tggcattttt aattcctacc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     9481 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggactacat tatttttctg
     9541 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     9601 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     9661 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggattg aaatttgcag
     9721 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     9781 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     9841 gtcgtgccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     9901 tgaacttcgc ggtgcagtat caaggtaaca acgaaggtgc cagtaatggt caggaaggca
     9961 ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg ctggggtctt tccacaacat
    10021 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
    10081 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
    10141 aatacgatgc taacaatatt tacctggcaa ccatgtattc agaaacgcgt aatatgaccc
    10201 cgtttggcga cagcgattat gctgttgcta acaaaaccca gaactttgaa gttactgcac
    10261 agtaccagtt tgattttggt ctgcgtccgg cagtctcttt cctgatgtct aaaggccgtg
    10321 acctgcacgc tgccggtggt gcagacaacc cggcaggtgt tgatgataaa gatctggtta
    10381 aatacgccga tgttggcgcg acttactatt tcaacaaaaa tatgtccacc tacgttgact
    10441 ataaaatcaa cctgttggat gaagatgaca gcttctacgc tgccaatggc atctctaccg
    10501 atgatattgt cgctttaggt ctggtttatc agttctaaat cctcctgccc gctgttatgg
    10561 cgggcttttt ctgcttattc ttcctccttt gatctaaatt aaaaatgtga actccgtcat
    10621 tacacaaaaa gtgtcatctg gcgttacact ttatgcggat actaaaacag gaggttttat
    10681 gaacagaacg attcttgtcc ctatcgatat ttccgattca gaattaactc aacgcgtgat
    10741 tagccacgtt gaggcagagg caaagattga tgatgcagag gttcatttcc tgacggtaat
    10801 accttcactg ccctactatg cctctctggg tttagcgtat tccgcagaat taccggcaat
    10861 ggatgacctg aaagcggaag ccaaatcgca actggaagag atcattaaaa aatttaaact
    10921 gccaaccgac agagtgcatg tccatgttga ggaaggctcg cccaaagacc gcattctgga
    10981 attggcgaag aagatccccg ctcatatgat catcattgct tcccatcgac cggatatcac
    11041 cacttatctg ctcggttcta acgccgcagc tgtcgtgcgt cacgcagagt gctccgtact
    11101 ggttgtgcgc tgacactaac gcccgcacat tgctgcgggc tttttgattc atttcgcaaa
    11161 tgtgctgaca ttttcccctc taatccgtac catacacgcc acagttttta tatcagattt
    11221 cttatgctgg gcgttccggc atgacgcata ctcttctgat gccatataac gaattgagtc
    11281 gcttttaaat gtcgcaaaat caagaaatta gtaagaaaga acaatacaac ctgaacaaat
    11341 tacaaaaacg tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag
    11401 aaggcgatcg catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaaa
    11461 ttctgcgcaa tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg
    11521 atcaaaagca accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg
    11581 ttgagtacaa gattgttgaa gagaacactt acggtatcgt gaaagagaag attccggagg
    11641 gcaaaaccac ttgctcactg tgttctcgtc ttcgtcgcgg tatcctttat cgtaccgcaa
    11701 cggaactggg agcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcaaacgt
    11761 tgttcttaaa tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg
    11821 atgatggcaa acatatcgtg attcgccctc tggcctactg ccgcgagaaa gatattcagc
    11881 gctttgccga tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta
    11941 acctgcaacg tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccggggc
    12001 gtatcgagac gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca
    12061 acctgttcga tttcaaaggc attacccacg gttctgaagt ggttaacggg ggtgatctgg
    12121 cgtttgatcg cgaagagatc cctctacaac cggcgggttg gcagcctgaa gaagatgaaa
    12181 atcagttgga tgagttacga ctgaatgtgg ttgaagtgaa ataacccgga tagcgcccga
    12241 tgcgcaagct tatcgggcta ctcttatgga ggccggagaa gacgcggcca gcgtcgcatc
    12301 cggcaatccc gaagaagatg ttgactcttg caccctgcga ttcaacattt cattatttta
    12361 ataaccgcac ccggcacgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt
    12421 tatgagcaac agcctgacgc accgcgacat agacatgcgc cggatgcacg gcgattttgc
    12481 caatatctgc gccatcaagc ccgatatctc ctgtcagcgc acctaataca tcacccgggc
    12541 gcattttggc ttttttcccg ccatcgatac ataacgttgc catttcggct tccagcggca
    12601 caatggaact attaactggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa
    12661 tgatattggc ccgctgtgct tcttccgggg cacagaaact gatcgccaga ccgctatttc
    12721 ctgcacgcgc tgtacgaccg atgcgatgta catgaacttc aggatcccac gccagctcaa
    12781 agttcaccac cagctcaagc gatttaatat ccagacctcg agcagcaaca tcagtcgcaa
    12841 ccagtacacg ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcacgttgct
    12901 ccagatcgcc gtgtaacgac aatgcacttt gccctacttc attcagcgcg tcgcagacag
    12961 cctggcaatc ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaaactta
    13021 ataacctttg caacaacgga attttgcctt tgctggatgt ctcataaaat tgttgttcaa
    13081 taggtggcag agcatctgtt gagtcaattt caatcgctaa aggatcgcgt tgcactcgtc
    13141 cgctgattgc ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg
    13201 caggcgcaaa acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcgat
    13261 cggcctcatc catcaccagc gtattcaacg catccagtga taccgtgcct ttttgcagat
    13321 gatccaacaa acggcccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac
    13381 gctgcatacc gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac
    13441 gcgccagccg acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca
    13501 ctaaagcctg ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag
    13561 ccgccgtttt gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg
    13621 ccggaagcgc ggcggcctgc accggcgtca tggttaaata acccaactca tcaaggttcg
    13681 tgagttgggc gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg
    13741 gtcatcgacg caaagtcagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa
    13801 tttaatcggt tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc
    13861 gggcactgga acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag
    13921 tgtcgtcata tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata
    13981 acaggcaaaa aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca
    14041 gtaattcaaa aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat
    14101 tcacctgcaa gagagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact
    14161 ttgcggcgtt tttgtataca gacagctggg aaaatttctt ttgttacaac catttactac
    14221 gatgcaacca taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa
    14281 atccgaattg ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca
    14341 gaaaggtact gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgcgcta
    14401 aattttcctg catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg
    14461 cgtcgatttc gtcaagcccg cgcccaaggc gatcggcaat atcctgcata cgacggcgtt
    14521 ggtcatcgct catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg
    14581 gtgccatata gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg
    14641 gtggaatttg ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct
    14701 cgataaattc gctggaatga tcggtcaacg catcacatac atccaccagc catcccccgc
    14761 aatcggtcgg acccgtgccc tcttccagat cgcttaccac atcgtccagc gccagcactt
    14821 tgcgttgtcg ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt
    14881 gatcggggcg ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac
    14941 cgagacggct gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg
    15001 gaagcagcgg tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga
    15061 gccagcaggg atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc
    15121 taccatccag catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg
    15181 cttccacagt gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta
    15241 cgcaacatct catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat
    15301 acaaaaagca aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca
    15361 ttatcagaat tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt
    15421 gataaaaata acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc
    15481 agtcctgtct ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg
    15541 cgactgttga tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc
    15601 tcacaaaaag aactgccaaa atacctctcc gatctgcaaa accaacacga tattatcgaa
    15661 atcctcaccg ttcaacgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga
    15721 aagctgacag aagcagaacc ggtgattatt ttcgaaggta tcgaagcacc ggcaacgctg
    15781 ggtttaaaag ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc
    15841 ttttttctga ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc
    15901 gtcgatgcta acctcgccgc gctcaatttc tatggctata accatgaaac gatgtgccag
    15961 aaacatacct gggaaataaa tatgctcggg cgacgcgtca tgcctatcat gcatgaaatt
    16021 tcgcatttac ccggtggcca taagcctttg aattttgttc ataaactggc ggatggttcg
    16081 actcgtcatg tgcagaccta tgctgggccg attgaaattt atggcgacaa gctcatgtta
    16141 tgtattgtgc atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc
    16201 caccatgacg cgatgaccgg attactgaat cggcgacagt tttaccacat taccgagcca
    16261 ggccaaatgc agcacctcgc catcgctcag gattacagct tattgctcat cgacaccgat
    16321 cgttttaaac acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc
    16381 ctcgcccgca ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc
    16441 gaagagtttg tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa
    16501 actatccgcg taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc
    16561 attggtgtgg cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat
    16621 gatgctttgt atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg
    16681 gatgcgtctc gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc
    16741 tgcgccgttt tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg
    16801 gtgcaatatg cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca
    16861 atattggcat gcgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa
    16921 ccatccgcca tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc
    16981 tggcgcaaaa gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc
    17041 accccatgtt gcaacccttc ccgccgaaac tccagcggct gacttagcgg gatgatgtcg
    17101 agaaatccag tggtgaggaa attatcaagt tgcagcgtgt cgatacgctg cggcgcacgt
    17161 tggttacgcg ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc
    17221 atggcgtcaa gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag
    17281 agcgcgatat gataaccaga ccgggtcggt ccaacaacgt attacccaaa tttccagtaa
    17341 taagttccaa atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg
    17401 atattaataa tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa
    17461 aggttaaaac cgttataaca cagtcaccgg cgcagaggag accatgccgg atttaagacg
    17521 cggatgcact gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta
    17581 tgcagtttta actttgcaga tagccgcatt ctcgccgaaa ttgcgtgatt aatgacgcgg
    17641 tcagtggtgt ctggcgacta tcacgccgct gaatcaaata ataggtcgct ttcggtaaaa
    17701 tttcgctaac cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt
    17761 caggtagtat gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct
    17821 cgcagactac gccgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaagtgtt
    17881 tgtagtagct gccgtgtggc gtcggcattg tccagctgta gtccagtaac tgtttgatcg
    17941 aacgggcacc aatggcggga tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta
    18001 atttctcaaa agtaaattcg tggtcgtagg gtccctgata ataggtattg atggtgaaat
    18061 ccagttcccc ctggcgcaat tcattaatca tcgataccag ttgcccttcc ataatgcgta
    18121 cttttacctg cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg
    18181 aaatactggc ccccatgccg atattaattt gccctgccag ttgcccttgt cgttggcgaa
    18241 tatcctcttg cgctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt
    18301 caccggcatc agttaacgtc acgcctttac tacggcgaaa aaagagttgt gccgccaacc
    18361 cttcttccag ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc
    18421 tcgctccgcg aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt
    18481 ttacctgaaa agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca
    18541 tctactttaa tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa
    18601 tcagttagtg ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga
    18661 cgggaaaggt tatggagtct ttgaatcaat ttattaattc gcttgcccca aaattatcgc
    18721 actggcgacg tgattttcac cactatgcag agtctggctg ggtggaattc cgcactgcca
    18781 cccttgttgc ggaagaattg caccagctcg gttattcact ggcgctgggc cgcgaagtag
    18841 ttaatgaaag tagccggatg ggattacctg atgaattcac tctacaacgc gaattcgagc
    18901 gcgctcgtca acagggtgcg ctagaacaat ggattgcggc ttttgaaggc ggtttcactg
    18961 gcatcgtcgc taccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata
    19021 tggacgcgct ggatctcagt gaagaacggg atgtcagcca tcgcccctac cgcgacggtt
    19081 ttgcgtcatg taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg
    19141 gacttgggct ggcgcatacc cttaaacagt tcgagtccgg actacatggc gtcatcaaac
    19201 tgatttttca acctgcagaa gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg
    19261 tagtggatga tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca
    19321 ccgtggtgtg cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg
    19381 ggaccgccgc tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag
    19441 cacaagcaac tcttgcactg catgcaatcg ccccgcacag cgaaggcgct tccagagtaa
    19501 acgtgggcgt tatgcaggca ggaagcggtc gtaatgttgt tcccgcctca gcgttgctga
    19561 aagtggaaac acgcggggcc agcgacgtca ttaatcaata tgtttttgaa cgtgcacaac
    19621 aagcgattca gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg
    19681 cagctaccgc cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcagctc
    19741 aggtcccggg ggtcaatcaa gccattgaac gtgttgaagc gcctgcgggt tccgaagatg
    19801 ccacattaat gatggcccgc gtgcagcaac atcaagggca agcctcctat atggtgtttg
    19861 gcacacagct ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg
    19921 ctattgccgt cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct
    19981 gatgcaggaa atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac
    20041 cgatattgcc gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc
    20101 ggagcatctg gcttcggcgc tggagtctgc aggcttcacc gttacccgca acgtaggcaa
    20161 tatcccaaat gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg
    20221 ggaatatgac gccctggcag gtttaagtca gcaagcaggt tgcgcgcaac ctacatccgc
    20281 gacgcccggt gaaaatggtc acggttgcgg acacaatttg ctgggaaccg ccgcctttgc
    20341 cgcagcaata gccgtcaaga aatggctgga acaatatggg caaggcggca cggtgcgctt
    20401 ttatggttgt cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt
    20461 atttgatgat gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa
    20521 tacccgcacg ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc
    20581 cgcgaattcc cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg
    20641 caccaacttc ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaga
    20701 tagcggcggg atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg
    20761 cgcccccgaa atgaccgatg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg
    20821 tgcggcattg atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta
    20881 tctcccgaat cgcaccttag aaaatgccat gtaccgagcc ctatcccatt ttggtactcc
    20941 ggaatggaac tgcgaagaac tggcttttgc gaaacaaatt caggctacgc tcaccctcaa
    21001 cgatcgtcaa aacagtctga ataatatcgc tgcaaccggt ggcgaaaacg gcaaggcttt
    21061 tgcactacgt catcgtgaaa cggtactggc gaatgaagtc gctccatatg ccgccaccga
    21121 taacgtgctt gcggcatcaa ctgatgtcgg cgacgtcagt tggaaactgc ctgttgccca
    21181 gtgtttcagt ccctgttttg ccgtcggtac cccgctacat acatggcaac tggttagcca
    21241 ggggcgaaca tctattgctc ataaaggaat gctgctggcg gcgaaaacta tagcagcaac
    21301 cacacttaat ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca
    21361 agttacggac acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt
    21421 aaaataacaa caacaacgca aacacaacaa acgaggaatg cccatgagta tgtcatccat
    21481 accgtcgtcc tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt
    21541 gccccatcct tttttgctct ttatctattt gattatcgta ctcatggtga cgacagcaat
    21601 tttgtcggcc tttggcgtca gtgcgaaaaa cccgaccgat ggtacgccgg tggtcgtgaa
    21661 aaacctgctc agtgtggaag tattacactg gtttttaccc aatgttatta aaaactttag
    21721 cggttttgct ccacttggag cgatcctggc gctggtttta ggtgccggtc tggcggagcg
    21781 cgtcggctta ctgccagcgt tgatggttaa aatggcatcg ca
//
LOCUS       DAYPHN010000074        21244 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000074 DAYPHN010000000
VERSION     DAYPHN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.074"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            128..766
                     /gene="rutR"
                     /locus_tag="ACG0BW_002635"
     CDS             128..766
                     /gene="rutR"
                     /locus_tag="ACG0BW_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFK7259585.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(806..4768)
                     /gene="putA"
                     /locus_tag="ACG0BW_002636"
     CDS             complement(806..4768)
                     /gene="putA"
                     /locus_tag="ACG0BW_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFK7259586.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVELALESAVNNAPIWFATPPAERAAILHRAAVLMESQIQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDERDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            4823..5032
                     /locus_tag="ACG0BW_002637"
     CDS             4823..5032
                     /locus_tag="ACG0BW_002637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259587.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            5191..6699
                     /gene="putP"
                     /locus_tag="ACG0BW_002638"
     CDS             5191..6699
                     /gene="putP"
                     /locus_tag="ACG0BW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFK7259588.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(6847..8190)
                     /locus_tag="ACG0BW_002639"
     CDS             complement(6847..8190)
                     /locus_tag="ACG0BW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259589.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVNANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGTLVKIAVSKNGVCTSTNIALELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGGEETHITNNGMREFGNMSPLAWRNDGELQQSCNTSNIPP
                     NTANDDINNTRQTTEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNDLGGVQGWCTRN
                     HFNIKNARNYLTDHGLNYAGEVKVNGPHEYAKFTLEHIRQWAALDKKVRKPVGYLEKW
                     CKKRNLSRTTARNYLKNDGLTALGELKLKGPQKCVSFTFGDIVQWANMSQEDRDRAGG
                     AHKWSKGRGFQWSTSRSYLKLSGLTVQGAKKLAWLNKSGNMSNPHYLAQPTSRRT"
     gene            9381..10211
                     /gene="efeU"
                     /locus_tag="ACG0BW_002640"
     CDS             9381..10211
                     /gene="efeU"
                     /locus_tag="ACG0BW_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFK7259590.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLF
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            10269..11396
                     /gene="efeO"
                     /locus_tag="ACG0BW_002641"
     CDS             10269..11396
                     /gene="efeO"
                     /locus_tag="ACG0BW_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFK7259591.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGDAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYSDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKGGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            11402..12673
                     /gene="efeB"
                     /locus_tag="ACG0BW_002642"
     CDS             11402..12673
                     /gene="efeB"
                     /locus_tag="ACG0BW_002642"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFK7259592.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQQVVWVTADQQEPAWAIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDTNDYLGSALLRV"
     gene            13299..14087
                     /gene="phoH"
                     /locus_tag="ACG0BW_002643"
     CDS             13299..14087
                     /gene="phoH"
                     /locus_tag="ACG0BW_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFK7259593.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            14674..15360
                     /locus_tag="ACG0BW_002644"
     CDS             14674..15360
                     /locus_tag="ACG0BW_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFK7259594.1"
                     /translation="MSFMVFVTCLTIAFVAGFAVARKLTLRKVASSVIITKSQPVPSS
                     ATSPAAKASTRSYTPQKTYRDSRWQERDELFQGMVAEGILLEGKEWLITRCEKIYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLNTIMTIARVPFVRASVTKLDEIPPVWDTRDKIRPL
                     AVATKGEPLH"
     gene            complement(15454..15541)
                     /locus_tag="ACG0BW_002645"
     tRNA            complement(15454..15541)
                     /locus_tag="ACG0BW_002645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15505..15507),aa:Ser,seq:gga)
     gene            15775..16713
                     /gene="ghrA"
                     /locus_tag="ACG0BW_002646"
     CDS             15775..16713
                     /gene="ghrA"
                     /locus_tag="ACG0BW_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFK7259595.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDPADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLKAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWSGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLIEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWLHPRVAITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            16768..17505
                     /gene="ycdX"
                     /locus_tag="ACG0BW_002647"
     CDS             16768..17505
                     /gene="ycdX"
                     /locus_tag="ACG0BW_002647"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFK7259596.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            17529..18083
                     /gene="ycdY"
                     /locus_tag="ACG0BW_002648"
     CDS             17529..18083
                     /gene="ycdY"
                     /locus_tag="ACG0BW_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFK7259597.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            18185..18676
                     /gene="ycdZ"
                     /locus_tag="ACG0BW_002649"
     CDS             18185..18676
                     /gene="ycdZ"
                     /locus_tag="ACG0BW_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFK7259598.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(18740..19573)
                     /gene="csgG"
                     /locus_tag="ACG0BW_002650"
     CDS             complement(18740..19573)
                     /gene="csgG"
                     /locus_tag="ACG0BW_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFK7259599.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(19600..20016)
                     /gene="csgF"
                     /locus_tag="ACG0BW_002651"
     CDS             complement(19600..20016)
                     /gene="csgF"
                     /locus_tag="ACG0BW_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFK7259600.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(20041..20430)
                     /gene="csgE"
                     /locus_tag="ACG0BW_002652"
     CDS             complement(20041..20430)
                     /gene="csgE"
                     /locus_tag="ACG0BW_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFK7259601.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(20435..21085)
                     /gene="csgD"
                     /locus_tag="ACG0BW_002653"
     CDS             complement(20435..21085)
                     /gene="csgD"
                     /locus_tag="ACG0BW_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFK7259602.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         5077 a         5083 c         5533 g         5551 t
ORIGIN      
        1 aaaagtggac taaacggtca aaacagttgc acataaaaca tgcatccgtg cgcgatgaaa
       61 gtgcagaagg tcgaggccgg gcaggggttt tgctatcctg ttgccaatct acaagagggg
      121 agagcgcatg acgcaaggcg cagtgaaaac aacgggtaaa cgttcgcgcg cagtgagcgc
      181 gaagaaaaaa gcgattctta gcgcagcact ggacactttt tcacaattcg gttttcacgg
      241 cacaaggctg gagcagatcg cagagttggc aggcgtttca aaaaccaatc tgctgtatta
      301 cttcccgtca aaagaggcgc tgtatatagc cgtactgcgg cagattctcg atatctggct
      361 ggcaccgtta aaagcgtttc gtgaagattt cgccccgctg gcggcgatca aagagtacat
      421 ccgtctgaag ctggaagtct cacgcgatta tccgcaggct tcacgtctgt tttgtatgga
      481 gatgctggca ggcgcaccgc tgttaatgga tgaactgacg ggtgatttga aagcattaat
      541 tgatgagaaa tcggcgctga ttgccggttg ggtcaaaagc ggcaaactcg cgccgattga
      601 cccgcagcat ttgattttta tgatttgggc ttccactcaa cattacgccg atttcgcccc
      661 tcaggtggag gcggtgacag gcgcgacgtt gcgcgatgag gtatttttca atcaaacggt
      721 tgaaaacgtg cagcggatta ttattgaggg gattcgacca cgttaaagat gccggaggag
      781 gttgtaacat cctccggctt cctgcttaac cgatcgtcat taaactggcg ttaccgccag
      841 cggcagcggt attcacactc agcgaacgct cgatatacag ccgttccagc aggatattgc
      901 tttcgccacg ggcaaaaccc tgcaccgaaa caattgcgcc atcccgcgcg gcaacggctt
      961 cacacaatgc gcgaagctga tccgaatcac cgtggaagat caccgcatca aacggttgag
     1021 cggttatatt ttccgctttc gccagttgaa tacgttcgct gactgccgat ggcaatgcct
     1081 tcactaactg acgatgcagc gcgtcatccg gccacagtac ctggctgccc accgccagca
     1141 cggcggcgag ctgagtcagc gcatcccgtt catcatccgc gatacacaat acgcgctcac
     1201 gcggcagcag cgtccaggtg ttgcgttcac ccgtcggccc tggcagcaat cgttgtgttc
     1261 ctgcctgcgc cagctcgcca tattgcgtac ataacgcctg caattctgga cggtttgccg
     1321 cccattcccg cagtgcattt agcggctgag tcaatgcggc tttcaactgc gcatcgaccg
     1381 gatactctgc atcctgacgc gcgagcgtca ctgccagcgc actttccggg cgattcgcca
     1441 gcagacggta gagatagagt ggaccgcctg ctttcggccc ggtgccggac aacccttcgc
     1501 cgccgaatgg ctgcaccccg accactgcgc ccaccatatt acggttaacg tacaggttac
     1561 cgacatgggc cgagccagtg acctgggcga tggtttcatc aatgcgcgta tggacgccaa
     1621 gcgtcagacc ataaccggaa gcgttaattt gctcgatcag ctccggcagc tggttacggt
     1681 tataacgcac cacatgcagc accggaccaa agacctcttt ttgcaattcg gcaaagtcat
     1741 ccagttcaat cagcgtcggg gcgacaaagg tgccgctttg ccattcacgg gcatcttcac
     1801 tgttttcccg cactgcctgg aacaccggac ggcctttact acgcatagtc tgaatatgac
     1861 gctcaatatt ggctttcgct tcgctatcaa tcaccggacc gatatcggtg gtcaggcgac
     1921 caggattacc catccggcat tcggccattg cgccgcgcag cattttcaac gtgtggtcgg
     1981 caatctcatc ttgcaggcac agcacacgca gtgccgaaca acgctggccc gcactgtcga
     2041 acgctgaggc cagcacatct attactactt gttcggtcag ggcggaggag tcgacaatca
     2101 tcgcgttcat gccgccggtt tccgcgatga gcggaatagg gcgaccctga gcgtccaggc
     2161 ggctggcgat attgcgttgc agtaacgtag cgacttcggt tgaaccggta aacatcaccc
     2221 cgcgcacgcg atcatcaccc gtcagttgcg cgcccacggt ttccccctga cctggcagca
     2281 actgcaccac gcctggcggt acacccgctt ccagcaaaat tgcgatccct tgcgcggcaa
     2341 tcagcggcgt ttgttctgcc ggttttgcca gcacgctgtt acctgccgcc agtgcggcgg
     2401 cgatctgccc ggtgaaaata gccagcggga agttccacgg actgatacac accacaggcc
     2461 ctaatggacg gtgggtttcg ttagcgaaat catcccgcac ctgtccggcg tagtagtgga
     2521 gaaaatcgac cgcttcgcgc acttcggcaa tggcgttact gaaggttttt ccggcctcac
     2581 gcactagaat accaatcagt tgctgtatct ggctttccat cagcacggca gcgcgatgca
     2641 aaatcgccgc gcgttcagcc ggaggcgtgg caaaccagat tggcgcgtta ttaaccgcac
     2701 tttccagcgc cagttctact tcacgcggcg tggcttcacg cacatagccc acaatatctt
     2761 tcggttccgc agggttaata acgggcgaca tctcaccagc cgctaccggt tgttccagca
     2821 ttggcaaggc ctgccatttt tgcagtgcac tattgagcag ggcagaagag agcgaggcca
     2881 gacggtgttc gttagcgaga tccagccctg ccgagttgtc gcgcccgtga ccgtaaagat
     2941 cgcgcggcag gggaattttc ggatgcggta atccagtttg cccttcctgt tgcgccagtt
     3001 tttctacagc agtgaccgga tcggcgacca gttcatccag tggcaaagag gtgtcggcaa
     3061 tacggttaac aaacgaggtg ttagcaccgt tttccagcag gcgacgcacc agatacgcca
     3121 gcagcgtttc atgggtaccg acaggagcat aaatacgaca cggacggtta agtttgccgt
     3181 cggcaacttt cccggtgacc tgctcataca gtggctcgcc cataccgtgc aggcactgga
     3241 actcgtactg acccgggtag tagttctgac ccgccagttg ataaattgcc gccagcgtat
     3301 gggcgttatg cgttgcgaac tgcgggtaga ttagattcgg caccgccagc agctttttcg
     3361 cacaggcgag atatgaaacg tcggtataca ccttgcgggt ataaaccgga taaccttcaa
     3421 ggccgtccat ctgtgcacgc ttgatttcac tatcccagta cgcgcctttc accaggcgaa
     3481 tcatcagacg gcgacggctg cgggtggcga gatcaatcag ataatcaatc accaacggac
     3541 agcgtttttg ataagcctga ataacaaaac cgatgccgtt ccagcctgcc agttccggct
     3601 cgaaacagag tttttccagc agatcgaggg agatctccag acgatcggcc tcttcggcat
     3661 cgatgttaat accaatatcg tactgacgcg ccagcagggt gagtgatttc agacgcgggt
     3721 aaagctcttc cattacccga tcatactggg cgcggctgta acgcggatgc agcgccgaca
     3781 gcttgattga aatgcccgga ccttcataga tgccacgacc attggacgct ttaccaatgg
     3841 cgtgaatcgc ctgctgatag gaaaccatat acgcctgtgc atctgcggca gtcagcgcgg
     3901 cttcgcccag catgtcgtaa gagtaacgga aacctttctc ttccagcttg cgggcattgg
     3961 ctaacgcttc cgcgatggtt tcgccagtga cgaactgctc gcccatcagg cgcatcgcca
     4021 tatccacacc tttgcggatc agcggttcac cgctcttacc gataatgcgg ttcagcgagc
     4081 gggagaggct ggcttcgtta tgggtggaaa ccagtttgcc agtaaacagc agcccccagg
     4141 tggcggcatt aacaaacaac gacgggctac gaccaatgtg tgactgccag ttaccgttgc
     4201 tgattttgtc gcgaattaac gcatcacggg tggctttgtc gggaatacgc aacaacgctt
     4261 ctgccagaca catcagcgcc acgccttcct gcgatgacag cgaaaactcc tgcaataacc
     4321 cctggaccat acctgcgcga ccactggcat ttttttgatt acgcagttta tcggccagtt
     4381 gatatgccag tttatgcgcc tgttcagcaa ctggctgcgg caggcgggct tgttccagca
     4441 gcatcgaaac cgcttcggtt tccgggcggc gataggcagc ggtgatcgcg gcgcgggaaa
     4501 ccgactgggg caaaatttgc tcggcaaagt cgaggaaagg ctggtgtggt tcctctgccg
     4561 gagtcggtgc ttcatcgctc tcattggcag cgccagaaag cagcgcaggc agctccggca
     4621 gagtatcgct gttttccagt tgttcgagat aagaaaaaat cgcctgctta attaaccagt
     4681 gtggtgtgcg atcgatacgt gtcgcggcag acttaatacg ctcacgcgtc gcgtcgtcca
     4741 gcttaacccc catggtggtg gttcccatgc cattactcct gttgttctga aaggtgcaac
     4801 ttaacgttat cgtgaaatat ccatgatgtt gcaactttgt gcaaccatgt taaatgtgac
     4861 ctgcgtagca agcttaaaaa tgaatgaaat gttaataaaa gaaatcgata tgacagggat
     4921 taaaaaaata actcagactt tttctctgcg gcagttaaca tttttgaaag gtgcaaccgc
     4981 aaaaaatgtg agagagtgca acctgatgaa aaatagtgtc gctgagcact aaaatttaat
     5041 gtaaatggtg tgttaaatcg attgtgaata accagcgctt ccggcaggat acggtcgccc
     5101 tggtaaaaca taaactctgt taccccgttc cggtggcaga tataacggca agtttcgaca
     5161 ttgccgataa taattttttg gagactttag atggctatta gcacaccgat gttggtgaca
     5221 ttttgtgtct atatctttgg catgatattg attgggttta tcgcctggcg atcaacgaaa
     5281 aacttcgacg actatattct gggcggtcgt agccttgggc cattcgtgac ggcattatcg
     5341 gcgggtgcat cggatatgag cggctggctg ttaatggggt tgccgggcgc ggtttttctt
     5401 tccgggattt ccgaaagctg gatcgccatt ggcctgacat taggcgcgtg gattaactgg
     5461 aagctggtgg ccgggcggtt gcgtgtgcat accgaataca acaataacgc cttaacattg
     5521 ccggattatt tcaccgggcg ctttgaagat aaaagccgta ttttgcgcat tatctccgcg
     5581 ctggtgattt tgctgttctt caccatttat tgcgcttcgg gcatcgtggc aggcgcgcgt
     5641 ctgtttgaaa gtacctttgg catgagctac gaaacggctc tgtgggcggg cgctgcggcg
     5701 acgatccttt acacctttat tggcggtttc ctcgcggtga gctggactga cactgtacag
     5761 gccagcctga tgatttttgc cctgatcctg acgccggtta tcgtcattat cagtgtcggt
     5821 ggctttggtg actcgctgga agtaatcaaa caaaagagca tcgaaaacgt ggatatgctc
     5881 aaagggctga actttgttgc cattatctcg ctgatgggct ggggactggg ttacttcggg
     5941 cagccgcaca tcctggcgcg ttttatggcg gcggattctc accacagcat tgtccatgcg
     6001 cgtcgtatca gtatgacctg gatgatcctc tgcctggcag gggcggtggc tgtcggcttc
     6061 tttggtattg cttactttaa tgagcacccg gcggtagcag gtgcggtaaa ccagaacgct
     6121 gaacgcgtgt ttatcgaact ggcgcaaatt ctgtttaacc cgtggattgc cgggattctg
     6181 ctgtcggcga ttctggcggc ggtaatgtca acgttaagtt gccagctgct ggtatgttcc
     6241 agtgcgatta ccgaagattt atacaaagcg tttctgcgta aacatgccag ccagaaagag
     6301 ctggtgtggg tagggcgtgt gatggtgctg gtggtggcgc tggtggcgat tgcgctggcg
     6361 gcgaacccgg aaaaccgcgt gcttggctta gtgagctacg cgtgggcagg ctttggcgcg
     6421 gcgtttggtc cggtggtgct gttctcggtg atgtggtcac gcatgacgcg caacggtgcg
     6481 ctggcgggga tgatcatcgg tgcgctgacg gttatcgtct ggaagcagtt cggctggttg
     6541 ggactgtacg aaattattcc gggctttatc tttggtagta tcgggattgt agtgtttagt
     6601 ttgctgggta aagcgccgtc agcggcgatg caaaaacgct ttgccgaggc cgatgcgcac
     6661 tatcattcgg ctccgccgtc acggttgcag gaagggtaag ctgaagagca ataatttatc
     6721 ttttgctttt taatccgaat gttccctgcc atgccgtata aattgcctgg cagggagcgt
     6781 tcgggggcaa ttgctatttg ccacctggct cagatgaaca aaattgccgc tatcggtacc
     6841 ttcagcttat gtcctgcggc tggtaggttg ggcgagatag tgtggattcg acatattccc
     6901 gctcttgttc agccacgcca gttttttcgc gccttgtacc gtcaatccgc tcaatttcag
     6961 gtaggatctg gaagtagacc actggaagcc gcgtccttta ctccacttgt gggctccccc
     7021 tgcgcggtca cgatcctcct ggctcatatt cgcccattgc acgatgtccc cgaaggtgaa
     7081 agagacacat ttctgcggcc ctttcagctt caactcaccc agggcggtaa gcccgtcgtt
     7141 cttcaggtag ttcctggcag tagtccgcga gaggtttctt ttcttgcacc acttttctaa
     7201 ataaccaacc ggttttcgca ccttcttgtc taaggctgcc cactggcgga tatgttcaag
     7261 ggtgaatttg gcatattcat gcggcccgtt tacctttacc tctcccgcgt agttcaggcc
     7321 gtgatcggtc aggtaattcc tggcattttt gatattaaaa tgattgcgcg tacaccatcc
     7381 ttgaactccc cctagatcgt ttcgcgcctg ctggtccata gccgcccatt ttcgcatgtc
     7441 gtcgacagtc aatttaacat attgctgtgg ctcgtccgcc tctggctggt cctcggtggt
     7501 ctgcctggtg ttgtttatgt cgtcattcgc agtatttggt gggatgttgc ttgtattaca
     7561 cgattgctgt aattccccat cgttacgcca cgccagcggg gacatatttc caaattctcg
     7621 cattccgttg ttggtaatgt gtgtttcttc tcctccgtat tgttgggccg gggagtagcg
     7681 agctgcttca tgcaccctgt tttgactttc atccgtgagt attggccgta cggaccagag
     7741 atcaaatgtt ccatactgac tgcgtgacat cagtgttgtg acgatggagt gaaacagctc
     7801 aagagcaata tttgtgctgg tgcatacacc gttcttactg actgcaattt tcacgagagt
     7861 gcccgtgttg tcatgctctt gcgtcttcgt gaaggtgatg gtgaaaccgg caacatcgaa
     7921 ggtgaacggt tcaggaagtt gcagatcatt tgcatttacc accggttgaa actgatgtcg
     7981 atgtagttcg gttgtcatcg caacagccag caagtgaacc tcttcaggaa ccagtggagt
     8041 aacagccatg cccgtgctgt agtccgcatc ccttttgata taattattca tctgcaccag
     8101 aagcgtcaaa gtttccagac aactactcgg gagttgctgc ggtggaatat aggttgctgc
     8161 gctcgtatta aggttggata ttggtctcat gtcatgctct ccgtatcctt acagggttaa
     8221 tgattcgtat taagtcgtag ccatccgcat ggctccgggc gattcagtct gcgcaccagg
     8281 atgtacactt cggatgcgtt cttgatgtag taatcaatcc gatcattctg gtgttaatgg
     8341 tgtaactacg aaagcacccg taaaaagcag gaggaatgcc agttattggc gaatttacaa
     8401 tgctgccatc ggggtgagtt tcatgaagat tgttattttt tttacgcctt gaatcatcgc
     8461 ggtaaataat aatttcttcc tgtgtttcat aaagtacgta gttacaatta ttcgtgagcc
     8521 ttgttgttaa ttcgatgtta gcccttggtc agagggaaaa ttaacgcgta agattttcaa
     8581 attcatcgac cgagaaatag atggattata tgctcgaccc taattacagg ggtcatctga
     8641 aaacagtggc gtctcatctt attccttctc tttctgtatt caatactagt ggggttgtaa
     8701 taatatagta aaaagtcgtg agtaaaaagc tttgattaat tatcagctta ttgattttta
     8761 tgtgttagtg tgtttgatga ttgattttca cgagaatctc ttacttttgg ttaagatttc
     8821 tatttttatt atatgtgact tttttaattc gcatcctgct tcgttttatc gtgatgtaga
     8881 attgtatata gcattagcca taaaatgata tcactggaaa tataataagg aggattataa
     8941 tttttggggt taattacgca ctatcaaaaa tcgctcattc caggcttaaa tacacgtttt
     9001 ctcctgttta ggttggggcg aaaaattcga tgaggagatt aggtataatt ttattcacaa
     9061 ccttatcact atctattaat aagcaaaaaa caacgttctt ttgtgaaacg acaaaatgaa
     9121 aattcaaccc ttcgggtcac gctgcagcga gtttgtcgcc accaacattc gtgcaagctt
     9181 catctgccgg gcgaaaagtc agctaaatac cttaaaataa tcgcttttat ctcctgattt
     9241 gaactcctcg tttttacatt ccaacatcat tttataaaca ttccgcttgt atttttcttt
     9301 accgtaatga taatcgctat cactgcgatt tacttttctt tgcatagatt gactcagaaa
     9361 aacgtttaag ggtgggtggc atgtttgttc cgtttctcat tatgttgcgc gaaggacttg
     9421 aagccgcgct gattgtcagt ttgattgcca gctatcttaa gcgtacccag cgaggccgat
     9481 ggattggtgt gatgtggatt ggcgtgctgt ttgccgctgc gttgtgcctg ggcttgggta
     9541 tcttcattaa cgaaaccacc ggcgaatttc cgcaaaaaga acaggaactg tttgaaggta
     9601 tcgtggcggt gatcgccgtg gtgatcctta cctggatggt tttctggatg cgcaaagtgt
     9661 cgcgcaacgt caaagtgcaa ctggaacagg cagtcgatag cgcattgcag cgtggaaatc
     9721 atcatggctg ggcgctggtg atgatggtct tttttgccgt tgcaagggaa gggctggagt
     9781 cggtcttttt cctgctggcg gcatttcaac aagatgtcgg gatctggccg ccgctgggtg
     9841 caatgctcgg tcttgctaca gccgtggtgc tcggcttcct gctctactgg ggcggtattc
     9901 gcctcaatct tggtgcattt tttaaatgga ccagcctgtt tattctcttc gtcgccgcag
     9961 ggctggcagc tggtgctatt cgcgcatttc atgaagccgg attgtggaac cactttcagg
    10021 aaatcgcctt cgatatgagt gcggtgctgt caactcactc gctgtttggc acgctgatgg
    10081 aagggatttt tggctatcag gaagcgccaa gtgtcagcga agtcgccgtc tggtttattt
    10141 atctcatccc ggcgctggtg gcatttgccc tgccaccacg cgcaggggcg acagcgtctc
    10201 gctccgcgta gcaaatacga cgcaaactct tgcttagtta caacatactt taaagggata
    10261 gtctcgtcat gaccattaac ttccgccgta acgcattgca gttgagcgtg gctgcgctgt
    10321 tttcttctgc ttttatggct aacgccgctg atgtgccgca ggtcaaagtg accgtgacgg
    10381 ataagcagtg cgaaccgatg accattacgg ttaacgccgg gaaaacacag ttcattattc
    10441 agaaccacag ccagaaggcg ctggagtggg agatcctcaa aggcgtgatg gtggtggaag
    10501 agcgggaaaa tatcgcccct ggttttagcc agaaaatgac ggcgaattta cagcctggtg
    10561 aatacgatat gacctgcggt ctgctgacta acccgaaagg gaagttgatc gtcaaaggtg
    10621 aagcaacggc ggatgcggcg caaagtgatg cgctgttaag tcttggtgat gcaattactg
    10681 catataaagc gtatgtcatg gcggaaacca cgcagctggt gaccgacacc aaagccttta
    10741 ccgacgctat taaagcaggc gatatcgaaa aagcaaaagc actgtatgcg ccgacgcgcc
    10801 agcactatga gcgcattgaa ccgattgctg aactgttctc cgatctggat ggcagcattg
    10861 acgcccgtga agatgattac gagcaaaaag ccgccgatcc gaaattcact ggtttccacc
    10921 gtctggaaaa agcattgttt ggcgacaaca ccaccaaagg aatggatcag tacgctgacc
    10981 agctttatag cgatgtggtc gatttgcaaa aacgcatcag tgaactggct ttccctccgt
    11041 caaaagtggt cggcggcgca gccggactga ttgaggaagt ggcagccagc aaaatcagcg
    11101 gtgaagaaga tcgctacagc cacaccgatc tgtgggattt tcaggctaac gttgaaggct
    11161 cgcagaaaat tgtcgatctg ctgcgtccac aactgcaaaa agccaacccg gaactgttgg
    11221 caaaagtcga tgccaacttc aaaaaggtcg ataccattct ggcgaaatac cgtactaaag
    11281 gcggttttga aacctacgac aaattgaccg atgccgaccg taacgcactg aaagggccga
    11341 ttactgcgct ggcggaagat ctggcgcaac ttcgcggtgt gctgggactg gattaagtgt
    11401 tatgcagtat gaagatgaaa acggcgtgaa tgaaccgtca cgccgacgtt tactgaaagg
    11461 aattggcgcg ctggcgctgg caggaagttg tccggtcgct catgcacaaa aaacgcaaag
    11521 tgcaccgggg acgctttcac cggatgcacg caatgagaag cagccttttt atggtgagca
    11581 tcaggcaggg atactgacgc cacaacaggc tgcaatgatg ctggtggcgt ttgatgtgct
    11641 tgccagcgat aaagccgatc ttgaacggtt gtttcgctta ttgactcagc gttttgcttt
    11701 tctgactcag ggcggagcgg caccagaaac gccaaatccg cgcctgccac cactcgattc
    11761 cggcattctt ggcggctaca ttgcgcccga taatctcacc ataacgttat cggtgggcca
    11821 ctcattgttt gatgagcgct ttggccttgc gccacagatg ccgaaaaagt tgcagaagat
    11881 gacgcgtttc cccaacgact cgctagatgc agcgttatgt catggtgatg tgttgctaca
    11941 aatttgcgcc aacacccagg atacggtgat ccatgcgctg cgcgatatca tcaaacacac
    12001 gccggatttg ctcagtgtgc gctggaagcg ggaagggttt atttccgatc acgcggcgcg
    12061 tagtaaaggc aaagagacgc cgataaattt gctgggtttc aaagacggca ctgccaatcc
    12121 cgatagccag aatgataagt tgatgcaaca agtggtgtgg gtgacggcag atcagcagga
    12181 gcctgcgtgg gctatcggcg gcagctacca ggcggtgcgg ttgattcagt ttcgggtgga
    12241 attttgggac agaacgccgc tgaaagaaca gcagacgata tttgggcgtg ataagcaaac
    12301 cggcgcgccg ctgggaatgc agcatgagca tgatgtgcct gattacgcca gcgatccgga
    12361 ggggaaggtg atcgcgctgg acagccatat ccggctggcg aatccccgca cggcggagag
    12421 tgagtccagc ctgatgctgc gtcgtggcta cagttattca ctgggcgtca ccaactccgg
    12481 acaactggat atggggctac tgtttgtctg ctatcaacac gatctggaaa aaggcttcct
    12541 gacagtacaa aaaaggctca atggcgaagc gctggaggaa tacgttaaac ctatcggcgg
    12601 cggttatttt tttgcgctgc cgggggtgaa ggacacgaac gattatctcg gaagcgcgtt
    12661 attgcgggtt taatgttttc aggcggataa ggcgttcgtg ccgcaccagg cgttcgtgcg
    12721 ctgatgcgac gcatgcgcgt cttatcatgc ctacaatcgg tgcgggtcgg taggccggat
    12781 aaggcgttca cgccgcatcc ggcaagcttt taccagccct gcgaatcctg ctgaaagcac
    12841 acagtttttt tatcactgtc atcactctgt catctttcca gtagaaacta atgtcactga
    12901 aatgatgttt tatagttaaa tataagtgaa tataatgttg caatgaatgc gagatctgtt
    12961 gtaattatta agtagcagcg gaagttcccg gcagtgatag tcagtcacta tggagatcgc
    13021 ggatggtaac gtcctgtagt ggacatgttt tagataatca acgcgccacc actcgcggag
    13081 atttctcctc cggtagtcat ctcgtcactt tgcattttca acctcatcct ttcttttctt
    13141 gtgttaccga cgccgtaaac ggtgcgcgta gccgtttttc ggcattttat ccaaaagcaa
    13201 actatggctt acaaggaagc caaccctctg atgttcgtgc gcataatcgc gctgccaacg
    13261 gcgcgtgtga tgaatacaaa caactcaagg tgctctccat gggaagacaa aaagcagtga
    13321 tcaaagctcg tcgcgaggca aaacgtgtgc tgagacggga ttctcgcagc cataagcagc
    13381 gtgaagaaga atcggtcacc tcgcttgtgc agatgagcgg cgtagaagcc attggtatgg
    13441 cccgcgacag tcgcgatacc tcgcccatcc tcgcgcgaaa tgaagcgcaa ttgcactatc
    13501 tgaaggctat tgagagtaag cagctgatat tcgccacggg cgaagccggg tgcggaaaaa
    13561 cctggatcag cgcagcaaaa gcggcagagg ccctgataca taaggatgtc gacaggatta
    13621 tcgtcacccg tccagttctg caagccgatg aagatcttgg cttcttacct ggagatatcg
    13681 cagaaaagtt tgctccctat tttcgtccgg tctacgacgt gctggtccgg cgcttagggg
    13741 cttcttttat gcagtactgc ctgcgaccgg aaatagggaa ggtggaaatt gcgccgttcg
    13801 cctatatgcg tggacgtacc tttgaaaatg cagtcgtcat tcttgacgag gcgcagaatg
    13861 tgactgccgc gcaaatgaaa atgtttttaa cccgcctcgg ggagaacgtg acggttatcg
    13921 tcaacggtga tatcacgcaa tgcgatttgc ctcgcggcgt gtgctccgga ttaagtgacg
    13981 cgctggaacg tttcgaagaa gatgaaatgg tcggcatcgt ccgattcggt aaagaggatt
    14041 gcgtacgttc ggcactttgc caacgtacgc tacatgcata cagttaagtg tattatcggc
    14101 gcagagcccg ggcgaaccgg gctttgtttt atttcattaa cagcaaagac tttcacttca
    14161 ggttaacgtt caggggggcg actttgagcg tgttgataag ctgaaatgaa ttctggttta
    14221 gatactctta ccaggcattt ttcgtcatag cacccgacca gtgtaatgcc gccagcgcgg
    14281 agagtttttc ttccagaagc ccaaaaagcg agtatctgcc tatctttttc cagccattca
    14341 acctgtacag gaggtctggt gccaattttg cgtatgaggt ttaatcaaca atgggccaaa
    14401 atttgactga ctgattaaca ccgaatatat tctcctgatt ttttttaggg ataatattaa
    14461 aaatataagg ttaatcttaa ttaatttttt ggctaatatt gtgtgttgtt attttttgtg
    14521 gacggcaagc cgcaatggca aaatagggat ggttgtaata atcaaaacat gatactaact
    14581 atatatagat cgttgaattg ttggcatgta actcgctaca gcgagtagcg ttagtaatga
    14641 aatataatca acatggatag gtgaagatta attatgagct ttatggtttt tgtaacttgt
    14701 ttgacaattg catttgtcgc cggttttgct gttgccagaa aattgacact ccgaaaagtt
    14761 gcctcatcgg taatcatcac taaatcgcaa ccagtccctt cctctgcaac ttcccctgct
    14821 gctaaggcgt cgacaaggag ctatacaccg cagaaaacct atcgtgactc tcgttggcaa
    14881 gaacgagatg agttatttca gggaatggtt gccgaaggta ttttattgga aggaaaagag
    14941 tggttaatta cgcgttgtga aaaaatctat tttgataatc tggagaaatg gtttggacat
    15001 tattgtcata tccattgcca ggtctctctg gggcgcttga tcaactttcc agaacaaccc
    15061 gagtttagcg aagaagaaag aaagcgtttc tttgcaattt ataatggaat ggcaatggat
    15121 tatgttctgg tttcaaagaa gaacaacaaa atagtctgtg ttattgagct ggatgatccg
    15181 agtcatgaaa gggcagatcg cattattcgt gaccgttggt tgaatacgat tatgactatt
    15241 gcacgggtgc cttttgtacg agcttctgtc actaaattgg atgaaatccc tccggtctgg
    15301 gatacccgtg acaaaattcg cccattagcc gtggcgacaa agggtgaacc gttgcattaa
    15361 ctaatgtacc ccggtcgggg cttctcatcc ccccggtgtg tgcaatatac gaaaaaaaaa
    15421 gcccgtacct ccgtacgagc tcttctttaa agatggcggt gaggggggga ttcgaacccc
    15481 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
    15541 caaattgtcg ttcctgtctt gctggaacgg gcgctaattt agggaaatca tgacctgagg
    15601 tcaacaaact tttttaaaaa atcgcgcgtt tattcaaact tcaatcaata tgcagtttta
    15661 ataagcgaaa tctgcttttt ttgccaccga ccacggattt gttatgctgg tggtctttgt
    15721 agatcataac gataagtgcg aataaatttc gcgcaacgct tttcgggagt ctgtatggat
    15781 atcatctttt atcacccaac gttcgatacc caatggtgga ttgaggcact gcgcaaagct
    15841 attcctcagg caagagtcag agcatggaaa agcggagata atgaccctgc tgattatgct
    15901 ttagtctggc atcctccagt tgaaatgctg gcagggcgcg atcttaaagc ggtgttcgca
    15961 ctcggggccg gtgttgattc tattttgagc aagctaaagg cacaccctga aatgctgaag
    16021 ccttctgttc cactttttcg gctggaagat accggtatgg gcgagcaaat gcaggaatat
    16081 gctgtcagtc aggtgctgca ttggtttcga cgttttgacg attatcgcat ccagcaaaat
    16141 agttcgcatt ggcaaccgtt gcctgaatat catcgggaag attttaccat cggcattttg
    16201 ggcgcaggcg tactgggcag taaagttgca cagagtctgc aaacctggcg ctttccgctg
    16261 cgttgctgga gtcgaacccg taaatcgtgg tctggcgtgc aaagctttgc cggacgggaa
    16321 gaactttctg catttctgag ccaatgccgg gtattgatta atttgttacc gaatacccct
    16381 gaaaccgtcg gcattattaa tcaacaatta atcgaaaaat taccggatgg cgcgtatctc
    16441 ctcaacctgg cgcgtggtgt tcatgtcgtg gaagatgacc tgctcgcggc gctggatagc
    16501 ggcaaagtta aaggtgcaat gctggatgtt tttaatcgcg aacccttacc gccggaaagt
    16561 ccgctctggc tacatcctcg cgtggcgata acaccacatg tcgccgcaat tacccgtccc
    16621 gctgaagctg tggagtacat ttctcgcacc attgcccagc tcgaaaaagg ggagagggtc
    16681 tgcgggcaag tcgaccgcgc acgcggctac taataaagca tcaggtttcc tgctatcctt
    16741 ggcgggaatt gaatacagga gagagttatg tatcccgtcg accttcatat gcataccgtt
    16801 gccagcacgc atgcatatag cacattgagt gattacattg cccaggccaa acaaaagggc
    16861 attaaacttt ttgcgatcac cgatcatggc ccggatatgg aagatgcgcc gcatcactgg
    16921 cacttcatta acatgcgtat ctggccgcga gtggttgatg gggttgggat cctgcgcggc
    16981 atcgaagcta acattaaaaa tgttgacggt gaaattgact gcagtggcaa aatgtttgat
    17041 tcgctggatc tgattattgc cggttttcat gagccggtgt ttgcgccgca tgacaaagcg
    17101 actaatacgc aagcgatgat cgccactatc gccagcggca atgtgcatat aataagccat
    17161 cccggaaatc cgaaatatga aatagatgtt aaagccgttg ctgaagcagc tgcgaaacat
    17221 caggtggcgc tggaaatcaa taattcctca tttttacact cacgtaaggg cagtgaagac
    17281 aactgtcgtg cggtagccgc agcggtacgt gacgctggag gttgggtggc attaggttcg
    17341 gattctcaca ccgcgtttac catgggtgaa tttgaagagt gtcttaaaat cctcgacgcg
    17401 gtagattttc cgccagagcg cattttgaat gtttctccgc gccgcttact gaacttcctt
    17461 gaatctcgcg gtatggcacc gattgcggaa tttgcagacc tttaattact agtaatggaa
    17521 atatattaat gaacgagttt tctatcctct gtcgtgtgct gggttcgctc tattaccgcc
    17581 aaccgcaaga tcctttactg gtgccgctgt ttaccctgat tcgtgagggg aaactggctg
    17641 cgaactggcc actggagcag gatgagttac tgacgcgttt acagaaaagc tgtgatatgg
    17701 cgcaagtctc tgccgattac aatgcgttgt ttatcggcga tgaatgtgct gtgccgccat
    17761 atcgtagcgc atgggttgag ggcgcgacgg aagcggaagt gcgcgctttt ctttccgaac
    17821 gggggatgcc attagcggat acgccagccg atcacatcgg cacattgctg ctcgcggctt
    17881 cctggctgga agatcagtca acggaagatg agagcgaagc actggaaaca ctgttcagtg
    17941 agtatctgtt accctggtgt ggtgcgttcc ttggcaaagt ggaggcccat gcaactacgc
    18001 ctttctggcg caccatggca ccgctaaccc gcgatgctat tagtgcaatg tgggacgagc
    18061 tggaagaaga ttctgaagag taaatgtgat ctgcatcact cataactgca acagataatt
    18121 tgttgttgca taaaatgtgt gctcgatctc attcatggcc gcgttttctg ctatcatgcg
    18181 cggcatgaac atacttctct ctattgcaat cacaacaggc attctctccg gtatctgggg
    18241 atgggtggct gtttccctgg gcttactaag ctgggcgggc ttcctgggct gtacggccta
    18301 ctttgcctgc ccgcaaggtg ggctgaaagg gctggcgatc tccgctgcaa ccctgcttag
    18361 tggcgtggtg tgggcgatgg tcattattta cggtagtgca ctggcaccac atctggaaat
    18421 tctcggttat gtcataaccg gtattgtcgc gtttctgatg tgtattcagg cgaaacagct
    18481 gctgctttca tttgttccgg ggacgtttat aggcgcatgc gcgacgtttg ccgggcaggg
    18541 tgactggaaa ctggtgttac cttcgctggc gcttggactg gtatttggtt acgcaatgaa
    18601 aaacagcggc ctgtggctgg cggcgcgtag tacgaagacc gctcaccgtg agcagcaaat
    18661 caaaaataaa gcgtgacggg cactcacgct ttcgcttaaa cagtaaaatg ccggatgata
    18721 attccggcat ttttatctat caggattccg gtggaaccga catatggcgg tatttcacca
    18781 gaatgtcatt ctgccgttct gctttatttt gcaaatccca cagaccacgg tcgataccat
    18841 cattaatcag gaaaatgacc cctgtttcga tagccgacat caggcacagc atcacgggtt
    18901 cgttcgaggt gtaacccact tccccttcca gcaagcgctg atagtcaata aagcggaaaa
    18961 ccccggcctg aacttcatag gaaagtatcg tcttactggt gttcaccgag gagaggatct
    19021 caccggtact cacattgacg acgcgcaggt tcacggcaat ctgatcgagc tggtattgcg
    19081 tgtcggcacc gatcccaaaa tatcttgccc caacaccgcc cgatttaacg ttgctttcat
    19141 aaccgataat cgaaccttca accatgatat ttgccgccgt taaagattgc agcgggattc
    19201 ggttattaat ggcaaccgtg ccgttttctt gtgccgcacg aataatcttg cgctcgttaa
    19261 gcaggttttg taagccctgg cgctccagcg gtataaacca gcgagaatct ttcaatgccg
    19321 tgaccagcat tgccgtggcg ctttgcggaa cagcagtgga gaagttactt gccgggtaag
    19381 gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc ttacccgtcg
    19441 gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat gtcggtctgg
    19501 cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca accaaaagaa
    19561 ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg ttgagttatt
    19621 ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg tcacgttcaa
    19681 ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca ccatgcggcc
    19741 cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact ggatggcctg
    19801 agtaaagtta tctaacgctg agggtgtttc aataccatag tcatcgttat agctcggatc
    19861 tttatacgag ttttgtgcct gggcgctatt taataaaaaa gcgccattat ttgggttacc
    19921 accaaagttt ggattacgga actgaaaagt catggttcca gcccaactta atggcgaaat
    19981 aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac aattttttat
    20041 ttagaattca tcatgcgcca aatcgcccgt acttaataac gcctgattta tctggcgacg
    20101 atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttttcga agtctctttt
    20161 caacgggaat aaaaaagtct ggaaaataac gtcctgattg accgttatag tgatccagct
    20221 tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt cactttccca
    20281 tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat ggtcagttaa
    20341 caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca gaaattctgc
    20401 cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta tcgtttgccc
    20461 aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta taaagatgcg
    20521 ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta gacgcgccga
    20581 tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat tccgtgctgt
    20641 tataacgata gttacctgaa tgcgtaatca ggtaactggc aagcttttgc gtaaagtagc
    20701 attcgccgcg caggacgcct tgcaacccat tgacaacacg ttcttgatcc tccatggcat
    20761 aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa tcttcaggcg
    20821 tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc tgccaataat
    20881 ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct gaagatatat
    20941 cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgccagcgat tgtttaaggt
    21001 gctgcaagag agctgtcgcc tgcaaagaag gtttggtgat caacaataat gtatgaccat
    21061 gaatactatg gacttcatta aacatgatga aaccccgctt tttttattga tcgcacacct
    21121 gacagctgcc tctaaaatag aagcaccaga agtactgaca gatgttgcac tgctgtgtgt
    21181 agtaataaat cagccctaaa tgggtaaaat ataaaactaa tggattacat ctgatttcaa
    21241 tcta
//
LOCUS       DAYPHN010000075        21121 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000075 DAYPHN010000000
VERSION     DAYPHN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.075"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..517)
                     /locus_tag="ACG0BW_002654"
     CDS             complement(<1..517)
                     /locus_tag="ACG0BW_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259603.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     HINSTFSGEGYNPEQFDLAQHQLQQINACHTNTTDTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTIFSDTNFIAE"
     gene            complement(1098..2099)
                     /locus_tag="ACG0BW_002655"
     CDS             complement(1098..2099)
                     /locus_tag="ACG0BW_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFK7259604.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGISVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKVIEKPAVNCCSTKAVASINPIKLAIKDALQQFKDVLPY
                     LVLGVLLGSFIYGFIPSQWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2207..2503
                     /locus_tag="ACG0BW_002656"
     CDS             2207..2503
                     /locus_tag="ACG0BW_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFK7259605.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2637..3062)
                     /gene="arsC"
                     /locus_tag="ACG0BW_002657"
     CDS             complement(2637..3062)
                     /gene="arsC"
                     /locus_tag="ACG0BW_002657"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFK7259606.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQRAFTKEDGEKVVDEAGKRLK"
     gene            complement(3075..4364)
                     /gene="arsB"
                     /locus_tag="ACG0BW_002658"
     CDS             complement(3075..4364)
                     /gene="arsB"
                     /locus_tag="ACG0BW_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFK7259607.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALLTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWFVLLFLLVGFFVLEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLAENGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(4407..6158)
                     /gene="arsA"
                     /locus_tag="ACG0BW_002659"
     CDS             complement(4407..6158)
                     /gene="arsA"
                     /locus_tag="ACG0BW_002659"
                     /EC_number="7.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HFK7259608.1"
                     /translation="MKFLQNIPPYLFFTGKGGVGKTSISCATAIHLAEQGKRVLLVST
                     DPASNVGQVFNQTIGNAITPVTAVPDLSALEIDPQQAAEQYRARIVDPIKGLLPEEVV
                     NSIREQLSGACTTEIAAFDEFTGLLTDASLLARFDHIIFDTAPTGHTIRLLQLPGAWS
                     SFIESNPDGASCLGPMAGLEKQREQYADAVEALSDPERTRLVLVARLQKSTLQEVART
                     HEELAAIGLKNQYLVINGVLPESGQQNDPLAMAIVQREQEALVNLPAGLSTIPADTLF
                     LQPVNMVGVPALKGLFNTRFEPAPLSGQVRQQFSENMSFSELVNDIARSEHGLIMLMG
                     KGGVGKTTMAAAIAVRLADMGFDVHLTTSDPAAHINTTLNGTLNNLQVSRINPQEETE
                     RYRQHVLETKGKDLDEAGKRLLEEDLRSPCTEEIAVFQAFSRVIREAGKRFVVMDTAP
                     TGHTLLLLDATGAYHREIAKKMGEKGHFTTPMMQLQDPQRTKVLLVTLPETTPVLEAA
                     NLQADLKRAGIHPWGWIINNCLSIAETRSSLLCQRATQERPQIEAVMARHAKRVALVP
                     LFAQEPTGIEKLRKLAS"
     gene            complement(6176..6538)
                     /gene="arsD"
                     /locus_tag="ACG0BW_002660"
     CDS             complement(6176..6538)
                     /gene="arsD"
                     /locus_tag="ACG0BW_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HFK7259609.1"
                     /translation="MKTLTVFDPAMCCSTGVCGSDVDQVLVDFSADVQWLKSRGVQVE
                     RYNLAQQPMSFVQNEKAKAFLEASGAEGLPLILLGGETVMAGRYPKRAELARWFGIPL
                     EKVGLAPTHCCGGKTDCC"
     gene            complement(6586..6939)
                     /locus_tag="ACG0BW_002661"
     CDS             complement(6586..6939)
                     /locus_tag="ACG0BW_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7259610.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRECGLLLDRKQGKWVHYRLSPHIPAWAAKIIEQTRLSQLDDVQAIARK
                     LAAANCSADGKASCS"
     gene            complement(7478..7651)
                     /locus_tag="ACG0BW_002662"
     CDS             complement(7478..7651)
                     /locus_tag="ACG0BW_002662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259611.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHCQSENVSELTG"
     gene            7679..7762
                     /gene="dinQ"
                     /locus_tag="ACG0BW_002663"
     CDS             7679..7762
                     /gene="dinQ"
                     /locus_tag="ACG0BW_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFK7259612.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(7816..9168)
                     /gene="gorA"
                     /locus_tag="ACG0BW_002664"
     CDS             complement(7816..9168)
                     /gene="gorA"
                     /locus_tag="ACG0BW_002664"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFK7259613.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(9240..10082)
                     /gene="rlmJ"
                     /locus_tag="ACG0BW_002665"
     CDS             complement(9240..10082)
                     /gene="rlmJ"
                     /locus_tag="ACG0BW_002665"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFK7259614.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            10285..12327
                     /gene="prlC"
                     /locus_tag="ACG0BW_002666"
     CDS             10285..12327
                     /gene="prlC"
                     /locus_tag="ACG0BW_002666"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFK7259615.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELTQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKVVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            12335..13087
                     /gene="rsmJ"
                     /locus_tag="ACG0BW_002667"
     CDS             12335..13087
                     /gene="rsmJ"
                     /locus_tag="ACG0BW_002667"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFK7259616.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(13136..14605)
                     /gene="dtpB"
                     /locus_tag="ACG0BW_002668"
     CDS             complement(13136..14605)
                     /gene="dtpB"
                     /locus_tag="ACG0BW_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFK7259617.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            14771..14824
                     /locus_tag="ACG0BW_002669"
     CDS             14771..14824
                     /locus_tag="ACG0BW_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259618.1"
                     /translation="MVLNVVLAGDFDCVRRP"
     gene            complement(14921..15355)
                     /gene="uspA"
                     /locus_tag="ACG0BW_002670"
     CDS             complement(14921..15355)
                     /gene="uspA"
                     /locus_tag="ACG0BW_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFK7259619.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            15746..16081
                     /gene="uspB"
                     /locus_tag="ACG0BW_002671"
     CDS             15746..16081
                     /gene="uspB"
                     /locus_tag="ACG0BW_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFK7259620.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(16152..17651)
                     /gene="pitA"
                     /locus_tag="ACG0BW_002672"
     CDS             complement(16152..17651)
                     /gene="pitA"
                     /locus_tag="ACG0BW_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFK7259621.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            17883..19085
                     /gene="yhiN"
                     /locus_tag="ACG0BW_002673"
     CDS             17883..19085
                     /gene="yhiN"
                     /locus_tag="ACG0BW_002673"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFK7259622.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(19400..20452)
                     /gene="yhiM"
                     /locus_tag="ACG0BW_002674"
     CDS             complement(19400..20452)
                     /gene="yhiM"
                     /locus_tag="ACG0BW_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFK7259623.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIILGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDIGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            20835..>21121
                     /locus_tag="ACG0BW_002675"
     CDS             20835..>21121
                     /locus_tag="ACG0BW_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259624.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGNVDKKHRSKIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKLWFYNSEPSIDIKEY"
BASE COUNT         5474 a         5218 c         5334 g         5095 t
ORIGIN      
        1 gttcagcaat aaaattagta tctgaaaata tggtttggac actgaatgtt atgggtattc
       61 cctcttctac gctaatttta gttatgattg ccttgtcggg tttaggatta gcgacaacaa
      121 tgattcgaca ttcagcactt ttgttttttt cgtatctaat gttattaatc tgattaagta
      181 ttgattgtgt ctgttgcgta agttgtgtcg aacttatcgt ttcaatgtta gtaataagac
      241 ttacaagttg agccacaact tttgagtagt ctgcatttgt atctgtagtg ttcgtgtggc
      301 acgcattaat ttgttggagt tggtgttgtg ctaaatcaaa ttgttcaggg ttgtatcctt
      361 ctccagagaa tgtggaatta atgtgttgtt gttcttccca attatcacaa ggccacttcg
      421 gtgtgtccgg cgttttaaca ttatcggtat ttgttgtgca gttataaata tcgccgtgat
      481 atgtatttat tatacctggg gtaaagtcaa ttgacataac attccttatt gttcatctat
      541 ttcgctaaat ttttgtaaat cggagaatta gagcaggaat ggattttctt ctacgcggaa
      601 ataaacacag tgcgtcatat cttcctgata taattacctt taacttccct ttcatttcca
      661 cccagtaata tttgtgtata aaaaaaaggc agtgatagta tcgtaaagga aaccgttaac
      721 aactatcaaa aagcgctcat tttaaaaagt tatgatgttc tccgatagtg tgtgaagtga
      781 tatttttgtg atgttttaga tgtgtaataa tgaaccaggt taaagtgtat ttaaaaatgt
      841 gttgtattgg cgactgaaat ctgcccttta aaagggggga tagcactgaa acaaagagac
      901 attgcttctc cgataaaatg tcagagaagc atgattgcgt ggtgattgtc gattgatgac
      961 ttactgacgg atccgtcatc attctcaaag cattagttaa gttatttgtg atttttaagg
     1021 attatcctat ctatgatttt ctactcagaa ggataaggca ctcacaatgc tccttatagt
     1081 ttactgctgg catctattta aaatagcatt tgagtcaaat agcccatcaa gatagccata
     1141 cctaatataa ccgtcaggaa tgcaactatc atcggtattc tgaacattga tttaagtaat
     1201 atcacttccg tcaggcttgc tccggcactg ccgatgatta atgccattaa tgctcccaga
     1261 cccattcctt ttgtcatcag aacagatgcc agagggataa ccgcctctgc ccggatatac
     1321 agcggaattc cgacaacggc gctcaatggg atggcgaagg gattatctgc ccctgcatga
     1381 gcggcaatcc actgtgaggg gataaagcca taaataaaag agcctagtaa tacccctaac
     1441 acaagatatg gcagtacgtc tttaaactgc tgcaatgcat ctttgatggc taattttatg
     1501 ggattaatac ttgccactgc tttggtactg caacagttaa ctgcgggttt ctcaatgact
     1561 ttagccgctg gactacagca actcactttt atgggcgtat atgtcgtccc tggcgaagtt
     1621 ttggctggag cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac
     1681 cccagagaat cgaggataat actggcaagt acggagatgc cggcggcgat aatcgcgtac
     1741 aacaaggtaa ctttccagcc aaaggttacc cacattagtc cgacgataat gggattaagt
     1801 aacggtgaaa caaataaaaa agtgagggtc ggaccgaacc cggctttcgc tgacaacaat
     1861 ccacgtaaca tggggattgt cgagcaacta cagaacgggg taacggctcc caacagagcc
     1921 gccaggagat agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc
     1981 accttttgtc ttatcaggct gacaccggca ctaatcacta taaacagcaa agaaagctcg
     2041 acggcgagaa acacaaacat ctctgcggca tcttgcagca tcgcaagcca actactcata
     2101 cgtaacctca ttgaatgaat ccatatttcc agaataatcg aaatgaaatt cagatcaaga
     2161 tatttctggt attatcgaaa tatcaaccag acaaaggggg tgtcatatgc agttggaaga
     2221 agttgctaaa gcgctgaaag agctaggtca cccgacccgt ttgttcatat tcaaacacct
     2281 ggtcaaagcg ggggaacaag gtttgccggt cggtgagttg caaaagcagc ttgggatacc
     2341 cggctctaca ctcagtcacc atatttcagc actggtatcg gtagggctgg tcaaacaaaa
     2401 ccgtgagagt cggacgctga tgtgtgtttc gcaatacgca atgctggaag cgataattga
     2461 attcttacgc gaggagtgct gcgtaaatag caaaattgcc tgatgcgcta cgcgctacag
     2521 atttcatgcc atatattgaa tttgcacggt tttgtaggtc ggataagacg cgttagcgtc
     2581 gcatccgaca taaacgaagc gcactttacc aactatcggc agtggatatc gccgctttat
     2641 ttcaggcgtt tacccgcttc atcaaccact ttctcgccat cttccttggt gaacgcgcgt
     2701 tgttgcgcat ctggcagaat ttccagcacc acttctgaag ggcggcacag gcgagttccc
     2761 agcggcgtca ccacaatcgg gcgattaatc agaatcgggt gctgaagcat aaagtcgatt
     2821 agccgatcgt cagtaaattt atcttctgca aggcccagtt cctcatacgg ctcgacgttt
     2881 ttacgcaaca gcgcgcgtac ggaaatcccc atatcggcaa tgagtttgac cagctcatcg
     2941 cgcgttggcg gtgtttccag ataatagata atggtcggtt ctgtaccgct gttgcggatc
     3001 atctccagcg tattacgcga cgtaccgcag gccgggttgt gataaatggt aatgttgctc
     3061 atatcaatat ctcattacaa agtgaaagag aggcgtagcg ccagcgcagc cagcgtcaca
     3121 aaaagcaccg gcagggtcat gataattcct gtacggaagt aatatcccca gctgatagtc
     3181 atattcttct gcgaaagtac gtgcagccag agtagcgtag ccaggctacc aattggggta
     3241 attttcggtc ccaaatcgca gccaatcacg ttggcataaa ccatcgcttc tttgataacg
     3301 ccagatgccg tgctgccatc aatggacaac gcgccaacca gtaccgtcgg catattgttc
     3361 ataatagaag agaggaaggc ggtgaggaat ccggtgccga gcgtcgcggc ccacaggccg
     3421 ttttccgcca gcacgttgag tacgccagaa agatattccg ttagtccggc attgcgcagg
     3481 ccataaacca ccagatacat gccgagcgag aagatgacaa tctgccaggg cgcaccgcgc
     3541 aggactttac ccgtattaat tgcatgaccg cgtttagcga cggcaaataa tatcagcgcg
     3601 cccactgccg caatggcgct taccggaatg ccgagcggtt ccaggacgaa aaatcccacc
     3661 agcagaaaca gtaaaacaaa ccagccagtt ttgaacgtag caggatcttt aatcgcttct
     3721 gcgggcgatt tcagcagcgc catgtcgtag ttctgcggaa tatctttgcg gaaataaaga
     3781 tgcaacatca ccagcgtggc aacaattgcg gcgatatcca ccggcaccat caccgatgcg
     3841 tattcgcgaa agccgaggcc aaagaaatct gcggaaacga tattcaccag gttggagaca
     3901 ataagcggca ggctggcggt atcggcaatg aacccggccg ccatcacaaa cgcaagcgta
     3961 gtgcctttac tgaatcctaa agccagcagc atggcgatga caattggcgt taaaatcagc
     4021 gccgcgccat cattggcaaa cagagctgca acggcggcac cgagcaggac aatccaggtg
     4081 aacagcaagc gaccgcgtcc attgccccag cgtgagacgt gcagcgccgc ccattcaaaa
     4141 aagccggact catccagcag caggctgatg ataatgacgg caataaacgc cgccgtcgcg
     4201 ttccagacga tattccacac caccggaata tcacccggat gaaccacgcc cgtaagcaac
     4261 gccagtaccg cgccgagcgt tgcactccag ccgatgccta aacctttcgg ctgccagata
     4321 accaatacga tggtcaggac aaaaatagcg cctgccagta acatacaccc tcctgacagg
     4381 gcgacatggt cgccctgtat aaaaaattaa cttgccagct ttctgagttt ttcgataccc
     4441 gtaggttctt gtgcgaatag agggaccagc gctacacgct tagcatgtcg ggccatcacc
     4501 gcctcaattt gtggtcgttc ctgcgttgct cgctgacaca gtagcgacga acgagtttcc
     4561 gcaatagaaa ggcagttatt gattatccag ccccagggat gaatccctgc gcgtttaaga
     4621 tcggcctgca aattagccgc ttccagtacg ggtgtggttt caggcagcgt aaccagcagc
     4681 actttggtgc gttgcggatc ctgaagttgc atcattggcg tagtaaagtg acctttttcg
     4741 cccatttttt tcgcaatttc acggtgataa gccccggtgg catccagcag taacagcgtg
     4801 tgaccggttg gtgcggtatc catcaccaca aaacgtttac ccgcctcacg gatcacgcgc
     4861 gagaacgcct gaaatacagc aatctcttcg gtgcagggag agcgtaaatc ctcttccagc
     4921 agtcgtttcc ccgcttcatc gagatctttt ccctttgtct caaggacatg ctggcgatag
     4981 cgctcggtct cttcctgcgg attgatcctg ctgacctgta ggttgttaag tgtgccgttt
     5041 agggtagtgt taatgtgggc agcaggatcg gaggttgtaa gatgcacatc gaatcccata
     5101 tctgccagcc tgacggcgat agctgcggcc attgtggttt tccccacgcc gccttttccc
     5161 atcagcatga tcagaccgtg ttcactgcgg gcaatatcat tcactaactc agagaatgac
     5221 atgttttcag agaattgctg gcgcacctgg ccagagagtg gggcaggctc gaaacgggta
     5281 ttaaaaagac cttttaatgc aggcacgcca accatgttca ccggttgaag aaagagggta
     5341 tctgctggaa tggtcgataa accggcgggt aaatttacca gggcttcctg ctcacgctga
     5401 actattgcca ttgctagtgg gtcattttgc tgcccggact caggcaatac gccgttgatc
     5461 accagatact gatttttaag accaatcgct gccagttctt catgagtgcg agcgacttcc
     5521 tgcaatgttg atttttgcag gcgtgcaacc agcaccaggc gggtacgctc aggatcggat
     5581 aatgcttcta ccgcatcagc gtactgctca cgctgttttt ccagcccagc catcgggcca
     5641 agacaggagg caccatcggg attactttca ataaaactgc tccaggcacc ggggagctgc
     5701 agaagtcgta tggtatggcc cgttggcgcg gtatcaaaaa tgatgtgatc aaagcgggcc
     5761 agcagagagg catcggtcag taaaccggtg aactcatcga acgccgcgat ctctgtcgta
     5821 catgctcccg aaagttgttc gcgtatacta tttacaacct cctcagggag aagtcctttg
     5881 atagggtcaa cgattctggc ccgatactgt tcagcggctt gctgagggtc aatttccagc
     5941 gctgacaaat cgggaaccgc tgtcacagga gtaatagcgt taccaatagt ttgattaaat
     6001 acctgaccga cattcgacgc ggggtcggtg ctgacaagca gaacccgttt tccctgttcc
     6061 gccagatgga tagcagttgc gcaggaaatt gacgttttac ctacacctcc cttaccggta
     6121 aaaaacagat aaggcgggat attctgtaag aatttcatat gtccccctga ccggctcaac
     6181 aacagtcggt ttttccgcca cagcagtgtg ttggtgccag ccccaccttc tccagcggga
     6241 tcccaaacca gcgggctagc tcggcgcgtt tcggataacg gcccgccatc acggtttcgc
     6301 ctcccagtaa gatcaagggc aatccttccg ccccggaagc ctcaagaaac gctttcgctt
     6361 tttcgttttg cacgaaactc atcggttgct gcgcgagatt gtaacgttcg acctgaacac
     6421 cgcggctttt cagccattgc acatctgcgg aaaaatcgac cagcacctga tcaacatcag
     6481 aaccacagac gccggtactg caacacatcg ccggatcaaa caccgttaac gtcttcatct
     6541 tcgacacctc acattcggaa aatcatatat atccaggcaa attttttaac tgcagcttgc
     6601 tttaccgtct gcagagcagt tggctgcggc cagcttacgg gcgatagcct gcacgtcgtc
     6661 cagttggctt aatcttgttt gctcaataat tttcgctgcc catgctggaa tatgcggcga
     6721 taagcggtaa tgaacccact taccttgctt gcgatccagc aatagcccgc attcacgcag
     6781 caatgccagg tggcgggaga tcttgggctg cgactggtcg agagcagtgc agagatcgca
     6841 gacgcataac tctcccagtt cgctgagcag taaaacgatg cccagacggg tttcatcggc
     6901 aagaattttg aacagttgga tgggtaacag aaatgacata ttgcgctcct gattgttgca
     6961 ggtagtgtct ctcttcgaat cggataagtc aaaaacatat atggtttaac gaatgtgtaa
     7021 gtgcagagga aggtaatagg tgtgaatttt gagttgggta ttcatttgaa aggaggtctg
     7081 aatcaggacg taaaaaaacc gactttgcgt cggtttttta ctcttcagcc tgagttggtg
     7141 gctctggctg gagtgagaga gctcttatct aacagctcaa tactattagc cagtggctaa
     7201 cattgcaaga attagcgatt tcttcagctg gcggaggggg cacttcggta tatatcgcag
     7261 tgacgattta tatactttca ctgggtcatc gtcatattaa gccttgtccg aatccggatg
     7321 taaaaaaacc gacttcgcgt cggttttttt actcttcagc cctgagttgg tggctctgac
     7381 tggagtgaga tagtcatcat ctaacgcatc aatgctaata ccatactgaa actattgcaa
     7441 ggacatgctg gttttataac ccgcaagtac tgtatgatta tccagttagc tctgagacat
     7501 tttcactctg gcaatgcgca taaacgcttt caaagtcctg gtcagaagta cgggtggtgc
     7561 cgttaactga tgctctggcc ggagtgagat agtcgtcatc taacaatgag acatgcgccg
     7621 tgacaggcag tggatgagta agcggatgca ttctcactcc atcgcatgga gaaaacgggt
     7681 gattgataaa gcaatcatcg ttcttggagc gttaattgcg ctgctggaac tgatccgctt
     7741 tctgcttcag cttctgaact gatagcggaa acgtaattaa gggctaagag cacactactc
     7801 ttagcccttt aacatttaac gcattgtcac gaactcttct gccgctgtcg ggtgaatggc
     7861 aaccgtgttg tcgaagtctt ttttggttgc gcccatcttc agcgccaccg cgaagccctg
     7921 caacatttca tccataccaa aaccaatgcc gtgaattccg acaatcttct cttccggtcc
     7981 aacgcacacc agcttcatgc ggcacggctg gcgatgagtg gtgacggcgg tatacattgc
     8041 ggtgaaagag gatttataca ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg
     8101 ttccgttaaa ccaacagtac caatcggcgg atggctgaag accacggtcg gaatgttgct
     8161 gtaatccaga tgctcatccg gcttgttatt aaacaggcgt tcagagagac ggcgacccgc
     8221 tgcaactgcc accggtgtca gttctactgc acccgtgtta tcgcccaccg cgtaaatacc
     8281 ttcaacgttg gtgttttgat atttatcgac gacgatatag cctttttcgt tggttttaac
     8341 gccagcggct tccaggttga tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag
     8401 gcaatccacc gtttcactgc gcccatcttc cagctccagc gtcaggctac catcagcatt
     8461 tttcactact gctttcggga tggcgttggt gtgcaactgc gggccttcgg cgttcatcac
     8521 ttcgaccagc gtttcagaga tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac
     8581 aaacagatgc gttttcgcgc cgaggccgtt aatcacgcca gccagctcaa cggcgatgta
     8641 acccgcgcca acaaccgcca cgcgctctgg caaagcagga agggcgaaga agccatcaga
     8701 atcaataccg tattccacgc ccggaatatc cgggtggctc ggacgaccgc cggtggcgat
     8761 gaggatatga tcggccgtga tggtttcacc gttaacttcc agcgttttgg catcaacgaa
     8821 gcgggcaaag cctttgatta catcaacgtt atttttaccg agcacgtttt cataggaagt
     8881 atgaattcgg tcgatatagg cggtacggct ggcgatcaac gtttcccagt tgaacttatt
     8941 gatggtggta tcgaaaccat aatccgggcc gtacatgtgg atcgcttcac ggatttgcgc
     9001 cgcgtgccac atcacttttt tcggcacgca gccgacgttc acgcaggtgc cgcccagctc
     9061 tttggcttca atcagcgcgc atttctggcc gtacatagcc gcgcggttga tggaggcgat
     9121 accgccgctg ccgccgccga tggcgatgta atcatagtgt ttagtcatga catattgtcc
     9181 ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat
     9241 tactccggta cgatccagct tacggtggcg tgcccggtgc ctgccggaac cagtttgctg
     9301 tgcagccacg gcagcacgtt attcatctgc tgttccagct tccacggcgg attaatcaca
     9361 atcatgccgg aagcggtcat gccacggcga tcgctgtctg gcagtaccgc cagttcaatt
     9421 tgcagaattt tgcgaatgcc ggtcgcttcc aggtcgtgga tcatgcgctt aatttgctgg
     9481 cgcagcacca ccggatacca cagtgcgtaa gtgccagtgg cgaaacgttt gtaaccttct
     9541 gctatcccgc tgaccaccgc ctgatagtcg gttttcattt catacggcgg atcgataagg
     9601 attaaaccac ggcgggaaac cggcggcagt ttggccttaa gctgctggaa gccatcggct
     9661 ttttcgacgc gcgcacggct gtctttctga aattcagaac gcagcaacgg gtaatcgctc
     9721 gggtgcagtt cggtcagttg caggctgtcc tgttcacgca gtaactgacg ggcaatcaac
     9781 ggtgaaccgg ggtagtaacg caactgaccg ctacggttaa agtgttttac cacattgatg
     9841 tacgcctcca gctctgcagg caaatcgtcc tgctgccaga tacgggcgat accttcgaga
     9901 tattcgccgg tacgctcggc atgttcgctg cctaactgat aacgcccggc ccctgcgtgg
     9961 gtatcgagat agagaaacgg tttatctttc tctttcagcg actcgatgat caggctctga
    10021 acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc
    10081 atgggtaaag gtgttccggt aagtaaaaat cggcccgtat tcggggcgca caaaagccta
    10141 tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat
    10201 tcactacact taaccccatg ctacacacat tatgtaaagc gcctgttgag cgcttcctta
    10261 acctctttaa ccaggactgc gctaatgacg aatccgttac tgactccctt tgaattgcct
    10321 ccgttttcta aaattctccc ggaacatgtc gttccagccg tgactaaggc gctgaacgac
    10381 tgccgcgaaa atgtggagcg cgtagtagcg caaggggcac cgtacacctg ggaaaatctc
    10441 tgccagccgt tggcggaagt ggacgatgtg ctggggcgta tcttctcccc ggtcagccat
    10501 ctgaactcgg tgaaaaatag cccggaactg cgtgaagcgt acgaacaaac cctgccgctg
    10561 ctgtcagaat acagcacctg ggtagggcaa catgaagggc tgtataaagc atatcgcgac
    10621 ctgcgcgatg gcgatcatta cgccacgctg aacacggctc agaaaaaagc ggttgataac
    10681 gcactgcgcg acttcgaact ctctggcatc ggtctgccga aagagaaaca gcagcgttac
    10741 ggcgaaattg ctacccgtct ttctgaactg ggcaatcagt acagcaacaa cgtcctcgat
    10801 gcgacgatgg gctggaccaa actcgttacc gacgaagcgg agctggcggg gatgccagaa
    10861 agcgcgctgg ctgcggcaaa agcccaggct gaagcgaaag agctggaagg ttatttgctg
    10921 acactggata tcccaagcta cttgccggta atgacctact gcgacaacca ggctctgcgc
    10981 gaagagatgt atcgtgctta cagcacccgc gcttccgatc aaggcccgaa cgccggtaag
    11041 tgggacaaca gcaaggtgat ggaagagatc ctcgcgctgc gtcacgaact ggcgcaactg
    11101 ctgggttttg aaaactacgc ctttaaatcc cttgccacta aaatggcaga aaaccctcag
    11161 caggtgctgg atttcttaac cgatctggca aaacgcgcgc gtccgcaggg cgaaaaagag
    11221 ctgacacaac tgcgtgcctt cgccaaagcg gaatttggcg tcgatgagtt gcagccgtgg
    11281 gatatcgcat actacagcga aaaacagaaa cagcacctct acagcatcag tgacgaacaa
    11341 ctgcgtccgt acttccctga aaacaaagtg gttaacggcc tgtttgaagt ggtgaaacgt
    11401 atttacggta tcaccgctaa agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt
    11461 ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca gcttctatct cgacctgtac
    11521 gcccgtgaga acaagcgcgg cggggcgtgg atggatgact gcgtaggcca gatgcgtaaa
    11581 gccgacggtt cgctgcaaaa accggtcgcg tatctgacct gtaacttcaa ccgcccagta
    11641 aatggtaaac cggcactgtt tactcacgac gaagtgatca ccctgttcca cgagttcggt
    11701 catggtctgc accatatgct gacccgcatt gaaaccgctg gtgtttccgg gatcagcggg
    11761 gtgccgtggg atgcggtcga actgccgagc cagtttatgg aaaactggtg ctgggagcct
    11821 gaggcgctgg cgtttatctc cggtcactat gaaaccggcg aaccgctgcc gaaggagctg
    11881 ctggataaaa tgctggcggc taagaactac caggcggcgc tgtttattct gcgccagctg
    11941 gagttcggcc tgtttgattt ccgcctccat gccgagttcc gcccggatca gggagcgaaa
    12001 atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg tagtaccgtc tccatcctgg
    12061 ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg gttatgccgc aggttactac
    12121 agctacctgt gggccgacgt gctggcggca gatgccttct cgcgctttga ggaagagggc
    12181 attttcaacc gtgaaaccgg acagtcgttc ctcgacaaca ttctgagccg tggcggttca
    12241 gaagagccga tggatctgtt caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg
    12301 ctggagcatt acggcattaa gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac
    12361 aggcaccgga gacggtgcct tatctgttct ggcggcccgc tgggggctgg agcacgatga
    12421 agacaacctg atggcgctgg tgttaacgcc ggaacatctg gaattgcgca agcgtgatga
    12481 gccaaaactt ggcggcatct ttgttgattt tgttggcgga gcgatggcgc accgacgcaa
    12541 attcggcggc ggacgtgggg aggcagtggc gaaagcggtg ggcattaaag gcgattattt
    12601 gccggatgtg gtggatgcca ctgcaggact tgggcgcgat gcctttgtgt tggcttcagt
    12661 cggctgccgc gtgcggatgc tggagcgtaa tccggtggtt gctgcattgc tcgacgacgg
    12721 cctggcgcgt ggttatgcgg atgcggaaat cggcggctgg ttgcaggagc ggttgcagtt
    12781 aattcacgct tccagcctga cggcgctgac cgatattact ccgcgcccgc aggtggtcta
    12841 tctcgatccg atgttcccgc ataagcagaa aagcgcgttg gtgaaaaaag agatgcgagt
    12901 ctttcagtcg ctggtggggc cggatcttga tgccgatgga ttgctggagc ctgcacgctt
    12961 gctggcgacc aaacgcgtgg tggtaaagcg cccggactac gcgccgccgc tggcgaatgt
    13021 cgccacgcca aacgcggtag tgaccaaagg gcatcgcttt gatatttatg ccggaacgcc
    13081 ggtgtaaaag taaagcaggg tagcgttatc gctaccctgc tttctgcaag aataattaat
    13141 ggctttcggg cgtcgcaatc attcgtttca gccacggcac catcagcagc atcaccaccg
    13201 caacgcccag cgtaaccagg ccaatcttac caaacacgtt ggtatagacg ggcaacgtct
    13261 caagcggatc ggtaatgttg tctggtactg cggtaaatgt tgccacatag ccgcccagca
    13321 agaacgcggc agcctgcgtc aggaaccaca tcccgagaat aaagcccatc aaatgctgcg
    13381 gcaccagggc agcaatcatc gccagaccca gggcgctaat aaacagttca cccaggctct
    13441 ggaataagta aaccagcaca ataaaccacg gcgatgtcag cccctgggca tccgcaaacc
    13501 acattcccgc agctgcagcc gtcaaaaagc ccagtgagca cataaacatg ccgagagtaa
    13561 atttcatcgg catagagaga tctttgcctt tgttacccag atgcgtgtaa atgcctgcca
    13621 gtattgggct ggcgagtacc acccagaacg ggttaagcgc ctggaagctg accgggttga
    13681 tggaaaagcc gagaatttca tgatgcacgt tgttgatggc aaagaagttc agcgacgttg
    13741 gcatctgggc gtagagaatg taaaacacca ccgcttcgag catcaggaca aaggcgacaa
    13801 acattttatt gcgcccggtt ttatccagct tgaatgcctg acgaaagaaa atgatggtga
    13861 cgacgatgga gagaacaatc agcaccagat tggcgacttc tacgttgtgc atcagccatg
    13921 cacagacgaa gatcatcacc acgctgccaa gtaacacgta caacagtttg ctgaagctca
    13981 tcggccggaa gtcgggttca gaaccaatgt ctttcaccat tccgcgacag gcgatgtaaa
    14041 ccaatagcgc gataattaat cctgcaccgc acaggttgta ggtgactgaa tagccgaatc
    14101 tatcagcgat cacaggggcc agcgataacg ctatcaacga gccgatgttg atcgacatat
    14161 agaacagggt aaatgcgcca tcaagccgcg gatctttcgg cggatagcac ttcgaaagca
    14221 agctggctgg gttagcttta aacaggccgt taccgacagc gatagtcccc agggcgatga
    14281 aaatcaggtc aggcttaagt agcgacaggc cggtcatgaa gtagccaatc gccagcacaa
    14341 gtgctccgag aacaatggtg cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa
    14401 tggaaatgag gccatagacc agcgcagcaa aagcaccaaa agtgacaaaa gcctgttctt
    14461 gcgagaatcc aagctgctta acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc
    14521 cgaatcgctc ccataactcg acaaaaaaaa tcatgaaaaa tgggcgaggt tgctgcagca
    14581 tccccatggg tgttgttgta ttcatattta ttaaccttcc aataccatcc tgaaaagtat
    14641 gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt cagagattct taatattgat
    14701 gcggtgaata gattaataca ctattacaac agaaaataac cagctgatca cactaatgat
    14761 ggaaaatatg atggtgttaa atgttgtatt ggcaggggat tttgattgcg tacgcagacc
    14821 gtaggccaga taaggtgttt acgctgcaag gtaacaaggc gcgccaaaaa gcaaaacgcc
    14881 cgcatatgcg ggcgttcagg aacacgtcgt agagggaaga ttattcttct tcgtcgcgca
    14941 gcggaacaat cagcatatca acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca
    15001 gtttgctcca gaagtcctgg tggtgaccac aaaccaccag atccatatcg tatttcttga
    15061 ttgcatcgac cagaacctgg cccagatcgc cgctgccgct cagggtttca gtgattgggt
    15121 agcctgcatt agtggaaagc tcggtcagcg catgatgtgt ctcttcagag atgcgtttct
    15181 gcatatcacc cagattcaca tcaataagcc cggtgtatag gtcagagtag tttacatcta
    15241 cgtggatcag agaaactttc gcattgtagg ggcgagccat agagactgct ttctctacca
    15301 gaactttgct ttccggggag agatcgaccg cgatgagaat gtgtttataa gccatagtgt
    15361 tactccttcc ataaagttgt cgatgactgg ccagctagcg tttcttgtgc cgttacgtca
    15421 cccgcgtcct gcgaactacg tgcgcggggc taacccagcc gtaaagattc agtgttaaga
    15481 ccatccttac cttatagcga cccggatgat ccgtcaatcc gccttgctta cccgttagat
    15541 aaacaaattt tcgacatgaa tatattgata gtggttaacc ttctggaaaa aaaacaacct
    15601 gatctcctac actatctata gagccgctcg gatgttggtc accacaaaag cggtttctcc
    15661 aggacctggt gttaactgat tggcagcgta tcgggagcgg tagccccggg gtggaactcc
    15721 gcgggcaggt cgccggggag gaggtatgat aagcaccgtc gcattatttt gggctttatg
    15781 tgtcgtttgc attgttaaca tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt
    15841 actgcgtaac tgcgatccat tgctctatca atatgttgat ggagggggct tttttacctc
    15901 acatggccaa cccaacaaac aggtgcgtct cgtttggtat atctatgccc aacgttatcg
    15961 cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct
    16021 gaccagcgca ttgtgtggtc tggtggtggt cagcctgatt gcattgatga tttggcatta
    16081 agcacttcag caaaaataac gcataaaaaa agcgggccag taaactgacc cgctcgtttt
    16141 ggtgcgtgcg attacaggaa ctgcaaggag agccagtaca gcccgccgga aagcagtacc
    16201 gcagccggaa gggtaaacac ccaggccatc agaatactgg ttacggtttt acgctgtaag
    16261 ccgccgccat ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtagtggaa
    16321 accggcatcc cggtataact tgccaggccg atggacactg ccgccgtcat ctgggcagac
    16381 atcccctgag cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca
    16441 cgacgccagc caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac
    16501 accggcgcat actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga
    16561 tcgtctgcac tcacgccagg catcttcacc actttgtcga tagtgtcaga aacgcacagc
    16621 ataatgcggc gcatctggct acgttgatca agcgacagct tatcgtagct ttccacatcg
    16681 gtagtcaaca tgcctttcag gcggttgagc gcgttaatgg tattcgacgg atggcaatgg
    16741 aactccgcag gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta
    16801 gcctgtttca gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca
    16861 cgggtacggg tgatttcgta gccagtggca ttcatattca ccacgaagcc tgctggtgcg
    16921 acgccaatca ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc
    16981 gaaaacgcca cgccgatagc ggaaaggatc agcgctatac gcgtccagaa cggcggcttt
    17041 ttcttgccgt ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg
    17101 gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca
    17161 ataggggaaa cgatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc
    17221 accactgacg tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc
    17281 agcgtatgag agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc
    17341 gccgccagca acatagagaa caccatggca aggccatgag acgatcccat attaagcagc
    17401 agatccgtcg gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca
    17461 cccaagaagt tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta
    17521 tagataacgg ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac
    17581 agcacaaaag ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac
    17641 aaatgtagca taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac
    17701 tttcgcggcg cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt
    17761 cactcaaaaa ataattatct tatataattc aggtaaatac ttccttttag taatattgat
    17821 gctggtgcga ccactgagga atctttacaa ttcacgcccg ttttttctta gaggagcgca
    17881 acgtggaaag gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg
    17941 cgctggcagg tcaggcagga cgccgggttc tgctgatcga taatggtaaa aaaccagggc
    18001 gcaaaatcct catgtctggc ggtgggcgct gcaactttac caatctttat gtcgaaccgg
    18061 gcgcttatct gagccagaat ccgcattttt gtaagtctgc actcgcgcgt tttacccagt
    18121 gggatttcat tgatctggtc aataaacacg gcatcgcctg gcacgagaaa acgttagggc
    18181 aactcttctg cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga
    18241 agggcaatgt gaccttcaga ttgcgtagcg aagtgctgag cgtggagaag gatgatacag
    18301 gcttcacgct tgaactgaac ggcatgactg tcggttgcga aaagctagtc atcgcgaccg
    18361 gtggactgtc aatgccgggg ctgggcgcgt cgccgtttgg ttataagatt gccgaacaat
    18421 ttggcctcaa cgtgctgccg acccgcgcgg gtctggtgcc attcactctg cataaaccgt
    18481 tgctcgaaga gttacaggtg ctggcaggcg tggcggtgcc ttccgtgatt accgctgaaa
    18541 acggcaccgt tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg
    18601 tgttgcaaat ttccagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg
    18661 atgtggacct cgaaaccttc ctgaatgaac agcgtaatgc acatccgaat cagagcctga
    18721 aaaacacact ggcggttcat ctaccgaagc ggttggttga acgtttacaa caactcgggc
    18781 aaatcccgga tgtttcgcta aaacagctca acgtgcgtga ccaacaggca ctgattagca
    18841 cattgaccga ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga
    18901 cgctcggcgg cgtggacacc aacgaactct cttcacggac gatggaagcg cgcaaagtgc
    18961 ctgggctgta cttcatcggc gaagtgatgg acgtcaccgg ctggctgggg ggctataact
    19021 tccagtgggc gtggtcgagt gcatgggctt gtgcgcagga tttgattgca gcgaagtcgt
    19081 cctgagctat ttcctaaaga tgaaaattat tagagtcgca ttaaaaatga gctaattttg
    19141 atagtggtta tcttgtgatt attttctaat gagcctgtga actgaaaccc tccatgctta
    19201 atataaggtg gaaggaaagg tgattaaaac tcccccagtg gccagatctt tataaaatat
    19261 gaacaggttg aaaaaatcag caactttgct gtttttttat acttaaccac tatgcattaa
    19321 ttctgcgtta tttcatgata cctgagaaaa accagcgtta cgcaaatggt caacgctggt
    19381 tttatccggt acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg
    19441 agaacaacgt aaagcatacc gctcccaaac cgaccagaac ccgcgaagga ataaaatacc
    19501 ctatgtcggt ctgcgccatt tctgcaagaa acgatgccag gaaaaggcaa aacaggcagg
    19561 tgaagacggg aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cagaccagtg
    19621 ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa atgaggataa
    19681 tgccgggagc cagtcgggcg cttgcatcgg agctgactaa cacgtatatg ccctgcagca
    19741 ccgtgattga gccgagaata ataacccaat aacaccacag ccagtgttct ttggatgaga
    19801 acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg
    19861 cggttaaccc aagcaatacg tgaccggcga cataatgcgg agttatgtcg gcactacgta
    19921 acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc
    19981 aattaccgat taatgaagaa taagcctgta ccggtgttcc atcgctcttg ctccctgctg
    20041 cgtttttggg aatgagcaga aagtgaccgg atgacgctgc cactgtcgat acacaggcgg
    20101 caatcatacc aacgccaaaa ataacatggc cggcaacaaa ctcgtctgcc atcacatcat
    20161 tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa
    20221 tggggaataa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa
    20281 atgcggtagt gaataatgcc aggcatatgg ctgccagaga aatcagcaca tgacccgcga
    20341 cgaaatactc actttgccca gaactttcca gcacaaagcc acctaacgcg atacaaatta
    20401 agcccataat taaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact
    20461 cctcaggtat ccctgctaat attgaaatgt gcattaatga taatttcagc ctgttaatac
    20521 aatgacgaga tatcattaaa gacaaatgca tagcataact atagcgggat ttactttgca
    20581 gagcgctact tttcagcgtt caaaaggcca tggtaaataa atatttatcc taagattaat
    20641 tcttattctg ttgtttttta caattatctt ccagggaggg attctttttc attatttggt
    20701 aaaagaccgg atggtatcgg tgcttaaaat catcgaacat ataagtatta ttgctttgta
    20761 tcatcgtaaa aatgtctttc agacatgtgt catttatttt gttactaaca agtcaaaaat
    20821 atcacgggtt atttatgaaa gtatttcaca atatatttaa gcacttctct tcaaaccatc
    20881 aagataagca ttcagataaa gttaacagtc atcaacacca tggcaatgtt gataaaaaac
    20941 atcgttcaaa gatagttgaa attgataagc tggataaaca ttcgcaaata gataacgatt
    21001 ttggcctgca tatcatctat ttcctgcaac atggctgctg gaacgtaact gaacatagtc
    21061 ctcagataga aaaactgtgg ttttacaata gtgagccatc tatagatatt aaagaatata
    21121 a
//
LOCUS       DAYPHN010000076        20615 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000076 DAYPHN010000000
VERSION     DAYPHN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.076"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            288..1535
                     /gene="mdtA"
                     /locus_tag="ACG0BW_002676"
     CDS             288..1535
                     /gene="mdtA"
                     /locus_tag="ACG0BW_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFK7259625.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTSEEKATSREYAKKGARS"
     gene            1535..4657
                     /gene="mdtB"
                     /locus_tag="ACG0BW_002677"
     CDS             1535..4657
                     /gene="mdtB"
                     /locus_tag="ACG0BW_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFK7259626.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFERIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGINMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            4658..7735
                     /gene="mdtC"
                     /locus_tag="ACG0BW_002678"
     CDS             4658..7735
                     /gene="mdtC"
                     /locus_tag="ACG0BW_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFK7259627.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7736..9151
                     /locus_tag="ACG0BW_002679"
     CDS             7736..9151
                     /locus_tag="ACG0BW_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259628.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAIGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSIDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9148..10551
                     /gene="baeS"
                     /locus_tag="ACG0BW_002680"
     CDS             9148..10551
                     /gene="baeS"
                     /locus_tag="ACG0BW_002680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFK7259629.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10548..11270
                     /gene="baeR"
                     /locus_tag="ACG0BW_002681"
     CDS             10548..11270
                     /gene="baeR"
                     /locus_tag="ACG0BW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFK7259630.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11450..11782
                     /locus_tag="ACG0BW_002682"
     CDS             11450..11782
                     /locus_tag="ACG0BW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFK7259631.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11930..13291
                     /gene="yegQ"
                     /locus_tag="ACG0BW_002683"
     CDS             11930..13291
                     /gene="yegQ"
                     /locus_tag="ACG0BW_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFK7259632.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPHGNINFTLEQMENAKGEAMPVAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13621..13938)
                     /gene="yegR"
                     /locus_tag="ACG0BW_002684"
     CDS             complement(13621..13938)
                     /gene="yegR"
                     /locus_tag="ACG0BW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFK7259633.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            14353..15252
                     /gene="yegS"
                     /locus_tag="ACG0BW_002685"
     CDS             14353..15252
                     /gene="yegS"
                     /locus_tag="ACG0BW_002685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFK7259634.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VRIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(15334..16107)
                     /locus_tag="ACG0BW_002686"
     CDS             complement(15334..16107)
                     /locus_tag="ACG0BW_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFK7259635.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(16213..17253)
                     /gene="gatD"
                     /locus_tag="ACG0BW_002687"
     CDS             complement(16213..17253)
                     /gene="gatD"
                     /locus_tag="ACG0BW_002687"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFK7259636.1"
                     /translation="MKSVVNDTDGIVRIAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMSIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSLEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(17301..18656)
                     /locus_tag="ACG0BW_002688"
     CDS             complement(17301..18656)
                     /locus_tag="ACG0BW_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFK7259637.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(18660..18944)
                     /gene="gatB"
                     /locus_tag="ACG0BW_002689"
     CDS             complement(18660..18944)
                     /gene="gatB"
                     /locus_tag="ACG0BW_002689"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFK7259638.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(18975..19427)
                     /gene="gatA"
                     /locus_tag="ACG0BW_002690"
     CDS             complement(18975..19427)
                     /gene="gatA"
                     /locus_tag="ACG0BW_002690"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFK7259639.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(19437..>20615)
                     /gene="gatZ"
                     /locus_tag="ACG0BW_002691"
     CDS             complement(19437..>20615)
                     /gene="gatZ"
                     /locus_tag="ACG0BW_002691"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFK7259640.1"
                     /translation="AALAFDRNSTRKVLIEATSNQVNQFGGYTGMTPADFREFVFAIA
                     DRVGFARERIILGGDHLGPNCWQQENADAAMEKSVELVKAYVRAGFSKIHLDASMSCA
                     GDPIPLAPETVAERAAVLCFAAESVATDCQREQLSYVIGTEVPVPGGEASAIQSVHIT
                     HVEDAANTLRTHQKAFIARGLAEALTRVIAIVVQPGVEFDHSNIIHYQPQEAQPLAQW
                     IENTRMVYEAHSTDYQTRTAYWELVRDHFAILKVGPALTFALREAIFALAQIEQELIA
                     PENRSGCLAVIEEVMLDEPQYWKKYYRPGFNDSLLDIRYSLSDRIRYYWPHSRIKNSV
                     ETMMVNLEGMEIPLGMISQYLPKQFERIQSGELSAIPHQLIMDKIYDVLRAYRYGCAE
                     "
BASE COUNT         4895 a         5530 c         5256 g         4934 t
ORIGIN      
        1 cttcatcatg accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca
       61 gcgtcccgct gaaatcgtcg gcttacctcc tttcgccatg caagcagtct atcgctaacg
      121 cgtagataaa atagtttcat gtgttattac tggatgcgtg ctcgcaaatg tgcccgtcat
      181 tcagacgatt cccgacagtg tttcataatt cctccatttt tctcccttat tggctggcta
      241 cactagtatc attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt
      301 ataaatcccg ttgggtaatc gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt
      361 tctggcaagg ccgcaatgac tcccggagtg cagccccagg ggcgacgaaa caagcgcagc
      421 aatcgccagc gggtggtcgc cgtggtatgc gttccggccc attagccccg gttcaggcgg
      481 cgaccgccgt agaacaggca gttccgcgtt acctcaccgg gcttggcacc attaccgccg
      541 ctaataccgt tacggtgcgc agccgcgtgg acggccaact gatagcgtta catttccagg
      601 aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgacccaagc cagttcaaag
      661 ttgcattagc acaagcccag ggccaactgg caaaagataa agccacgctt gccaacgccc
      721 gccgtgacct ggcgcgttat caacaactgg taaaaaccaa tctcgtatct cgtcaggagc
      781 tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa
      841 gcgtcgccag cgcgcagctg caactcgact ggagccgcat caccgcacca gtcgatggtc
      901 gcgttggtct caagcaggtt gatgttggta accaaatctc cagtggtgat accaccggga
      961 ttgtggtgat cacccagacg caccctatcg atttagtctt taccctgccg gaaagcgata
     1021 tcgctaccgt agtacaggcg caaaaagccg gaaaaccgct ggttgttgaa gcctgggatc
     1081 gcaccaactc gaagaaatta agtgaaggca cgctgttaag cctcgataac caaatcgatg
     1141 ccactaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcactgtttc
     1201 ccaatcagtt tgttaacgcg cgtatgttag tcgacaccga acaaaacgcc gtggtgatcc
     1261 ccaccgccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa
     1321 acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc
     1381 gcgcaggtat ttctgcgggc gaccgcgtag tgacggacgg aattgatcgc ctgaccgaag
     1441 gggcgaaagt ggaagtggta gaagctcaaa gcgccaccac ttcggaagag aaagccacca
     1501 gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc cgagcagcac
     1561 aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc tgatggtggc
     1621 gatcttactc gccgggatta tcggttatcg tgccctgccc gtttcggcgc tgccggaagt
     1681 ggactatcca accattcagg tggtcaccct ctacccaggt gccagcccag atgtcatgac
     1741 ctctgccgtt accgcaccgc tcgaacgcca gttcgggcag atgtccggcc tgaaacagat
     1801 gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc taacattacc
     1861 gctggatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca acctgttgcc
     1921 gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc cgccaatcat
     1981 gacgctcgct gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata tggtggaaac
     2041 ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg
     2101 ccagcgtccg gctgttcgcg tcaaacttaa cgcccaggcg attgccgccc tcggcctgac
     2161 cagcgaaacc gtgcgcaccg ccattaccgg tgctaacgtt aactcggcta aaggtagcct
     2221 cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga
     2281 atatcgccag ctaatcatcg cctaccagaa cggcgcaccg attcgtctgg gcgatgtcgc
     2341 aaccgtagag caaggtgcag aaaatagctg gcttggggcg tgggcaaata aagaacaggc
     2401 cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg ccgacagcat
     2461 ccggcagatg ctgccacagc tcaccgagag tctgccgaaa tcggtgaagg tgacggtact
     2521 ttctgaccgt accaccaata tccgcgcatc ggtcaacgat acccagtttg aactgatgat
     2581 ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc cggcgaccat
     2641 cattcctggc gtcgcagtgc cgctgtcgtt aattggcacc tttgcggtta tggtgtttct
     2701 cgatttttca atcaataacc tgacgctgat ggcgttaact atcgccaccg gattcgtggt
     2761 cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc
     2821 gctggcggcg gcgctcaaag gcgcaggtga aatcggtttt accattatct cgctgacctt
     2881 ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg ggcgactgtt
     2941 ccgcgaattt gctattacac tggcggtagc gattttgatc tcagccgtgg tgtcgctgac
     3001 cctgacgccg atgatgtgcg cacggatgct cagccaggag tcgctgcgta aacagaaccg
     3061 cttctcccgt gcctcagaga aaatgttcga aaggataatc gccgcctatg gtcgcggact
     3121 ggcgaaagtg ctgaatcatc cgtggctgac tttaagcgta gcgctcagca cgctgctgct
     3181 tagcgttctc ttgtgggtgt ttattccgaa aggtttcttc ccggtacagg acaacggcat
     3241 tattcagggt actttgcagg caccgcaatc cagctccttt accaatatgg cccagcggca
     3301 acgccaggtc gcggatgtga ttttgcagga tccggcagtg caaagtctga cctcatttgt
     3361 tggcgttgat ggcactaacc cgtcgctgaa cagtgcgcgt ttgcagatca acctcaaacc
     3421 gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtgga
     3481 taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gacctgacca tcgatactca
     3541 ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg cgctcagtac
     3601 ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg atgtctccag
     3661 cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg
     3721 tctggggatc aacatggcgg atgtcgataa cgccctgtac aacgcgtttg gtcagcggct
     3781 gatttccact atttatactc aggctaacca gtatcgcgtg gtgctggaac acaacactga
     3841 aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg acggtggcgt
     3901 ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgctct ccatcaacca
     3961 tctggatcag ttcccggtaa cgaccatctc ctttaacgtg ccagataact attcgctggg
     4021 cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg tggatatcac
     4081 cacgcagttc cagggcagca ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct
     4141 gattgtcgcg gcggtggtgg cgatgtatat cgtactcggc attctttacg aaagctttat
     4201 tcacccgatc accattctct cgacgttgcc aacggcaggg gtcggcgcac tgctggcgtt
     4261 gctgattgcc ggtagcgaac tggatgtaat cgccattatc ggcattattt tgctgatcgg
     4321 tatcgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg agcgcgagca
     4381 aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc gtccgatcct
     4441 gatgactact ctggcggctc tgcttggcgc gttgccgctg atgttaagta ccggggtcgg
     4501 cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgatag tcagccaggt
     4561 gctgacgctg tttaccacgc cggtgattta tttgctgttc gaccgcctgg cattgtggac
     4621 caaaagccgc tttgctcgtc atgaagagga ggcgtaagtg aagttttttg ccctcttcat
     4681 ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg gcatactggg
     4741 cttccgtatg ctgccggtcg ccccactgcc gcaggtcgat tttccggtga ttatggtcag
     4801 cgcctcgctg cccggtgcgt caccagaaac aatggcgtct tccgttgcca cgccgctgga
     4861 acgttcgctt gggcgcattg ccggggtcag tgaaatgacc tccagcagtt cgctcggcag
     4921 cacgcgcatt attttgcagt ttgattttga ccgtgatatc aacggcgcag cgcgcgatgt
     4981 gcaagcggcg atcaacgccg cgcaaagttt gctgcccagc gggatgccaa gccgtccgac
     5041 ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctgacgctaa catccgatac
     5101 ttattcgcaa ggtgaactgt acgattttgc ctcgacgcag ctggctccga cgatttcgca
     5161 aatcgacggt gtcggtgatg tcgatgttgg cggcagctcg ctacctgcgg tgcgcgtcgg
     5221 gctgaacccg caggcgctgt ttaatcaggg cgtctcgctg gacgacgtac gcactgccat
     5281 cagcaatgcc aacgtgcgta aaccgcaagg ggcgctggaa gatggcactc accgctggca
     5341 gatccagact aacgatgaac tgaaaactgc cgctgaatat cagccgctga ttattcatta
     5401 caacaacggc ggcgcggttc gtctgggcga tgtagcgacg gtgaccgact cagtgcagga
     5461 tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttgctgatga tccgcaagct
     5521 gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac cggagttgca
     5581 ggagaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc ccactattcg
     5641 cgcctcgctg gaggaagtcg agcaaacgct gattatctcg gtggcgctgg tcattctggt
     5701 ggtcttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg cggtgccggt
     5761 ttcgctgatt ggtacgtttg cggcgatgta cctgtgcggt ttcagtctca ataatctttc
     5821 gttaatggcg ctcaccattg ctactggttt cgtggtggat gacgccatcg tggtgctgga
     5881 aaacattgca cgtcatctgg aagcgggaat gaaaccgttg caagccgcac tgcaaggtac
     5941 tcgcgaagtc ggttttacgg tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc
     6001 gctgctgttg atgggcggat tgccgggccg actgttacgc gagtttgccg tgacgctttc
     6061 tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta acgccgatga tgtgtggctg
     6121 gatgctgaaa gccagcaagc cgcgcgagca aaagcgactg cgtggttttg gtcgcatgtt
     6181 ggtagccctg caacaaggct atggcaagtc gctgaaatgg gtgctcaatc atacccgtct
     6241 ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tggctatata tctcgatccc
     6301 gaaaaccttc ttcccggagc aggacaccgg cgtgttgatg ggcgggatac aggcggatca
     6361 gagtatttcg tttcaggcga tgcgcggtaa gttgcaggat tttatgaaaa ttatccgtga
     6421 cgatccggcg gtggataatg tcaccggctt taccggcggt tcgcgggtga acagtgggat
     6481 gatgtttatc accctcaagc cacgcgacga acgcagcgaa acggcgcagc aaattatcga
     6541 ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat ctgttcctga tggcggtaca
     6601 ggatattcgc gttggtgggc gtcagtcgaa cgccagctac cagtacacgt tgttatccga
     6661 cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc
     6721 ggaactggcg gacgtgaact ccgatcagca ggataacggc gcggagatga atctggttta
     6781 cgatcgcgac accatggcac ggctgggaat cgacgtacag gccgccaaca gcctgttaaa
     6841 taacgccttc ggtcagcggc aaatctcgac catttatcag ccaatgaacc agtacaaagt
     6901 ggtgatggaa gtggatccgc gttataccca ggacatcagc gcgctggaaa aaatgttcgt
     6961 tatcaataac gaaggaaaag cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa
     7021 tgccccacta tcggtgaatc atcagggatt atcggcagcc tcgaccatct cgttcaacct
     7081 gccgaccggg aaatcgctct cggacgccag tgcggcgatc gatcgcgcaa tgacccagct
     7141 tggtgtgcct tcgacggtgc gcggtagttt tgccggcacg gcgcaggtgt tccaggagac
     7201 gatgaactcg caggtgatcc tgattatcgc cgccatcgcc acggtgtata tcgtgctggg
     7261 tatcctttac gagagttacg tacatccgct gacgattctc tccactctgc cctcggcggg
     7321 cgttggagcg ctgctggcgc tggagctgtt caatgccccg ttcagcctaa tcgccctgat
     7381 agggatcatg ctattaatcg gcatcgtaaa gaaaaacgcc atcatgatgg tcgattttgc
     7441 gcttgaagcc caacggcacg gtaacctgac gccgcaggaa gctattttcc aggcctgtct
     7501 gctgcgtttt cgcccgatta tgatgactac cctggcggcg ctgtttggcg cgctgccgct
     7561 ggtgttgtcg ggcggtgacg gctcggagct gcgccaaccc ctggggatca ccattgtcgg
     7621 cggtctggta atgagccagc ttctgacgct gtataccacg ccggtggtgt atctcttttt
     7681 cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac
     7741 agatcttccc gacagcaccc gctggcaatt gtggattgtg gctttcggct tctttatgca
     7801 gtcgctggac accaccatcg tcaacaccgc gcttccctca atggcgcaaa gcctcgggga
     7861 aagtccgttg catatgcaca tggtcattgt ctcttatgta ctgaccgtgg cggtgatgct
     7921 gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc aatattttct ttaccgccat
     7981 cgtgctgttt actctcggtt cactgttttg cgcgctttcc ggcacgctga acgaactgtt
     8041 gctggcacgc gcgttacagg gcgttggcgg tgcgatgatg gtgccggtcg gcaggttgac
     8101 ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg atgacctttg tcacgttacc
     8161 cggtcaggtc gggccgctgc tcggtccggc gctcggcggc ctgctggtgg agtacgcgtc
     8221 gtggcactgg atctttttga tcaacattcc ggtgggaatt atcggcgcga tcgccacact
     8281 gatgttaatg ccgaactaca ccatgcagac gcggcgcttc gatctctccg gctttttatt
     8341 gctggcgatt ggcatggcgg tattaaccct ggcgctggac ggcagtaaag gtacaggttt
     8401 atcgccgctg gcgattgctg gcctggtcgc agttggcgtg gtggcactgg tgctttatct
     8461 gctgcacgcc agaaataaca accgcgccct gttcagtctg aaactgttcc gcactcgtac
     8521 cttttcgctg ggcctggcgg ggagctttgc cggacgtatt ggcagtggca tgttgccctt
     8581 tatgacaccg gttttcctgc aaattggcct cggtttctcg ccgtttcatg ccggactgat
     8641 gatgatcccg atggtactcg gcagcatggg aatgaagcga attgtggtac aggtagtgaa
     8701 tcgctttggc taccgtcggg tgctggtggc gaccacgctg ggtctgtcgc tggtcaccct
     8761 attgtttatg actaccgctc tgctgggctg gtactacgtt ttgccgttcg tcctgttttt
     8821 acaagggatg gtcaactcga cccgtttctc ctccatgaac accctgacgc tgaaagatct
     8881 cccggacaat ctggcgagca gcggcaacag tctgctgtcg atgattatgc aattgtcgat
     8941 gagtatcggc gtcaccatcg ccgggctgtt gctggggctt tttggttcgc agcatgtcag
     9001 catcgacagc ggcaccacac aaaccgtctt tatgtacacc tggcttagca tggcgtttat
     9061 catcgccctt ccggcgttca tctttgccag agtgccgaac gatacgcatc aaaatgtagc
     9121 tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc gacccggtat taccggcaaa
     9181 ctgtttctgg cgattttcgc cacctgcatt gtcttgctga tcagtatgca ctgggcggtg
     9241 cgtatcagtt ttgagcgtgg ctttattgat tacatcaaac acggcaatga acagcgatta
     9301 cagctgttaa gtgatgcgct tggcgagcag tatgcgcagc atggtaactg gcgcttcctg
     9361 cgcaacaacg atcgctttgt ctttcagatc ctgcgttcat ttgaacacga taattcggaa
     9421 gataaacccg gcccgggtat gccgccgcac ggctggcgta ctcagttctg ggtggtcgat
     9481 caaaacaaca aagtgctggt tggtccgcga gcgccgattc cacctgacgg tacacggcga
     9541 cccattctgg tcaacggtgc ggaagtgggt gcggtgattg cctcccctgt tgagcgactg
     9601 acccgcaata ctgatatcaa tttcgataaa caacagcggc aaaccagctg gctgattgtc
     9661 gccctggcta cgttactcgc ggcactcgcc acctttctgc tggcgcgtgg tctgttggcc
     9721 ccggtcaaac ggctggtgga cggcacgcac aaactggcgg cgggcgattt cactacccgc
     9781 gtgacaccca ccagtgaaga tgaactgggc aaactggcgc aagacttcaa ccagcttgcc
     9841 agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta tggccgatat ttctcacgaa
     9901 ctgcgtacgc cattagcggt gctgcgcggt gaactggaag ccattcagga tggcgtgcgt
     9961 aaattcacgc cggagacggt ggcgtcttta caggcggagg tcggtacact gaccaaactg
    10021 gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca
    10081 ccggtagatt tgatcccact gctggaagtg gcgggcggcg catttcgcga aagattcgcc
    10141 agccgtggcc tgaaactgca attttccctg ccagacagta ttaccgtatt tggcgatcgc
    10201 gaccgtttaa tgcagttatt caataactta ctggaaaaca gcctgcgcta cactgacagc
    10261 ggcggtagcc tgaaaatctc tgccgagcag cacgacaaaa cggtgcgact gacctttgcc
    10321 gacagcgcgc cgggcgtcag tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc
    10381 accgaaggtt cccgcaaccg agccagcggc ggttccgggc tggggctggc gatttgcctg
    10441 aacattgttg aagcacataa tggccgcatt attgccgccc attcgccttt tggcggggta
    10501 agcattacag tagagttacc gctggaacgg gatttacaga gagaagtatg accgagttac
    10561 caatcgacga aaacacaccg cgtattttga tcgtggaaga tgaaccgaag ctggggcagt
    10621 tgctcattga ttatctgcgt gctgcgagct atgcgccgac gcttatcagc cacggcgatc
    10681 aggtactgcc gtatgtgcgc cagacaccac cggatctgat cctgctggat cttatgcttc
    10741 ctggcaccga tggcctgacg ctgtgccggg aaattcgtcg tttttctgac attccgatcg
    10801 tgatggtgac agcaaaaatc gaagagatcg accgcctgct ggggctggag attggtgcag
    10861 atgactacat ctgcaagccg tacagcccac gggaagtggt agcgcgcgtc aaaaccattt
    10921 tgcgccgttg caaaccgcag cgtgagttgc agcaacagga tgctgaaagc ccgttgatta
    10981 tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat gcttgacctg acgcctgcgg
    11041 aatttcgtct gctgaaaacg ctctctcatg aaccaggaaa agtgttctcc cgcgagcaat
    11101 tgctcaatca tctttatgac gactaccgcg tagtaaccga ccgcaccatc gacagccaca
    11161 ttaaaaacct gcgtcgcaag ctggaatctc tcgacgccga acagtccttt atccgtgccg
    11221 tttacggcgt cggttaccgc tgggaagccg acgcctgccg catcgtttaa catttttacc
    11281 tgccggagaa ggcattcacg ccgcatccgg caacataatc ccacgcatgg tcagcaaact
    11341 gacattatct ctccgccctg gcaggcacca gaaaatcgct catactttaa tcggtaaaca
    11401 gtccttttag atgctgtttt cgatacgcaa tttcaatcaa ggagtcatta tggctggttg
    11461 gtttgaactc agtaaaagca gtgataatca atttcggttt gtgctaaaag cgggcaatgg
    11521 tgagactatc cttaccagcg agctgtatac ctcaaaagcc tctgcggaaa agggcatcgc
    11581 gtcggtgcgt agcaacagcc cgcaagaaga acgctatgag aaaaaaacgg caagcaacgg
    11641 caaattctat ttcaatctga aagccgctaa tcatcaaatt atcggctcca gccagatgta
    11701 cgccaccgcg caatctcgtg aaaccggaat tgcctccgtt aaagccaatg gcacaagcca
    11761 gacggtgaaa gacaatacgt aattacgatg ccgggcgtgg gttgttggcg tccggcgctt
    11821 taccagctcc cccctacagc gctacaatgc ccgcccttaa agtgggggca ctcccctggc
    11881 cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta tgtttaaacc
    11941 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
    12001 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaatttaa
    12061 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
    12121 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
    12181 agtggtggag atggggccgg atgcgctgat tatgtccgat ccggggctga ttatgctggt
    12241 gcgcgagcac ttccctgaaa tgccgatcca tctctcggta caggctaacg ccgtgaactg
    12301 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
    12361 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga tcgagatctt
    12421 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
    12481 caagcgcgac ccgaaccagg gcacctgtac caacgcctgc cgctgggagt acaacgtcca
    12541 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
    12601 aaatgttgag ccgacgctgg gtatcggcgc gccaaccgac aaagtcttta tgatcgaaga
    12661 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
    12721 gaactcgaaa gatctgcgtg ccatcgccca cgttgaacgc ctgaccaaaa tgggcgtgca
    12781 ttcgctgaaa atcgaaggcc gtaccaaatc tttctactat tgcgcacgca ccgcgcaggt
    12841 ttatcgtaaa gctatcgatg acgccgctgc gggcaaaccg ttcgatacca gcctgctgga
    12901 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
    12961 cgatgattat cagaactacg aatacggtta ttcagtctct gaccgccagc agtttgttgg
    13021 tgagtttacc ggtgagcgca agggcgacct cgcggcggta gcggtgaaaa ataaattctc
    13081 cgttggcgac agccttgagc tgatgacgcc gcatggcaac attaacttta cccttgagca
    13141 gatggaaaac gccaaaggcg aagcaatgcc ggtcgcaccg ggcgatggtt atactgtgtg
    13201 gatcccggtg ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
    13261 ggaaaccacg cgtaaccccc acggtaagtg attaatttcg attatttttc ccggatggaa
    13321 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
    13381 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
    13441 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccat cttccattga
    13501 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctca
    13561 cacaggttcc ggctacgcac agccagaacg cgcatgtttg acgcttacca aaaaatattc
    13621 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
    13681 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgagctg catcattttt
    13741 tactatattt tctgcaccag aaattctgcc cctggctaaa gaagcttcgg cttcggtgta
    13801 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
    13861 acttgtctca tcattatgta cagcacaccc agacataaga aaaacactaa ttaatgatat
    13921 agcagctatc tttttcatct taccttcccc cattaaatac caacgacacg ctctggtgtt
    13981 aaaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
    14041 acatttccta cataagtagg aattacatac acggaggccc ttccgggtaa ccccatgggg
    14101 gggttaatat atttttaata gtatattgaa gatgccactg tttagttgaa tattaggtat
    14161 atgctctttt ttgaaattta cctatggcta ccgttaacat tcactgtccc cattgcaagc
    14221 tcctggtggt aaccactgaa tcctccatac ttgaactgat tttttatcct ccgactttca
    14281 tcctgttctg actccacctt ttcttttctg ctctacacta tctacagacc aatcataaag
    14341 gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact
    14401 gacaatttac cattgcgcga agcaattatg ttgttgcgtg aggaaggaat gacgatccat
    14461 gtgcgggtca cctgggagaa aggcgatgcc gcacgatatg tagaggaggc ccggaagttg
    14521 ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg
    14581 ttgattcagt gtgaggggga tgacataccc gcgctaggaa ttttgccatt aggaaccgcc
    14641 aatgattttg ccaccagtgt aaggattcct gaggcactgg ataaggcgct gaaactggca
    14701 attgccggtg acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt
    14761 aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa
    14821 gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa
    14881 ccggaccgtt gtgagatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt
    14941 ggtattggta acgggcgtca ggccggtggc ggtcagcaat tgtgcccgaa cgcgttaatt
    15001 aacgatggct tactgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta
    15061 tcaacattaa aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt
    15121 gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagcggacaa
    15181 aactttcata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgccca
    15241 ttgttgcgtt aatcacacta ttctctcttg aaccagggag aatccgtaat gccggatgcg
    15301 gtgtaaaaac tttatccggc atacagagca atactacacc gtaattaatt tcacccgtgt
    15361 cttctgcaat gccagtttat ccacctctga cacggcatca tcggtaataa taatgtcaat
    15421 tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg aatcgagaac
    15481 ggcaatcact ttatttgcgg ctgtaaccat cgccccgcta atcgaataac cttcattaaa
    15541 ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt
    15601 aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgtgttt tatggcggac
    15661 ggtcccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa atgctgccgg
    15721 gagactgttg gtaatcaccg tgatattttt ggcggtcatt aatccttcgg cgataagcat
    15781 tgtcgtactt ccgctgtcga gaataacggt catcccttcg tggatcattt tgactgccgc
    15841 ttgcgctatt ctgttttttg gcgcactggc gagctgaaat cgctctttaa acccaacttc
    15901 ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt
    15961 aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc
    16021 aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat
    16081 cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt
    16141 aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc
    16201 tggcccgcgg tttcagggaa tgagcaatac tttgcccggc atagcattac gagcgatgtc
    16261 acgcaccgcc tgggcgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct
    16321 taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct
    16381 ggagtagttc atccaactgc cgataaccgt cagctcttta cgtaatattt tgccaaacgt
    16441 tgccgatgtt aaatgcaaat cctgatgcaa cgtgcccacc agcgccagct gggcatgagg
    16501 cccggcaatc tctaccgcca gttcgacggt ttgcgggacg ccagccgtct cgaggataag
    16561 ctgattaaag cgcagctcgc gtaaaacgcc ctgcatttgc ggcgcgctca tttcaaggct
    16621 gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc
    16681 gatagccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat
    16741 ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc
    16801 atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaatagaca tatccgtggg
    16861 tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac catcacgccg
    16921 cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggaca
    16981 agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac
    17041 accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata
    17101 atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat
    17161 ttttacccgt acctcatcct gatgtttaat ttcaggaatg acgctttctg caatgcgcac
    17221 gataccatca gtatcattca ccactgattt cataaaaact cctgattgtt aagggggatt
    17281 acctccccct caggtaaaaa ttattctgcg agaacggctt tctcttgttt aataaaacca
    17341 cgcgctctac gccaggtcat gaaaataccg gtcagataaa ttgcgccgat aataatgaaa
    17401 ccgggaatat tttgcgggga gaaaacctga atcagtaacc aggtaatggg agaaccgccc
    17461 tgatccattg aagccaccat acccccggct tttaacgccc cagcattagc cgccagttgg
    17521 gtgtgcaaac caatagtttg cgtcgcgatc cacagggtga tgctcataat gatgacaccc
    17581 gagattaagg tgcggaacag atttccacga tgcacggcaa ctgccatcgc cacaaagaag
    17641 ccgatagttg caagatcgcc aaacggcagc acctgattac ccggcacaca aacagcaatt
    17701 aaaatggtga gcgggataaa aatcaggctt gccgatacca ccgccgtatg ccccagcagt
    17761 aatgccggat ccaggccaat caggaactcc tgaccaccga acttcgcctg caaacggcta
    17821 cgcgcctgct tcgcaatagg tgttaagcca tccatgatgg gtttaatcac tcgtggcatc
    17881 agcagcataa ctgccgccgt ttttaccgcc agctgcaata cacctttgac atcgtaaccc
    17941 gcgaggatgc cgataatcag ccccatcaca aaacccacgg tgacaggttc gccaaatggg
    18001 ccaaacttgc gctgaatatc atcggcacta aatttaattc ggttaacgcc tgggattttc
    18061 tcaatgatag catcgaccag caccgcaatc ggccccagat acgccgacgt accgtgcgga
    18121 atagcaatgc cttccagctc aaagaaattt cgggtatcgc gggcaaacca gtcgccgagc
    18181 ttataaacaa acgccgcgtg aattaccaca cccgccatcc ctatcatcca tgaaccggtt
    18241 gccagatgca gcaacgcgcc ggtgaaggtc atatgccaga tattccagat atcaacattt
    18301 accacccgcg tcatacgggt cagtaacatc gccacgttaa ccagaatcgc aatcggaatc
    18361 gccaccagcg caatttgcga cgcccaggtc attggtgaag agcccggcca gccgacatcg
    18421 accacatgca gattcaggtc gaaattttcc gccatcgctt tcgccgccgg accaatggaa
    18481 tccagcatta agccaatcac aaggccaatg ccaacaaagc caatcccgat atgcagaccc
    18541 gctttaaagc aatcgcctgc cttcatgcct aatattttag aaaaaataat gatgacaatc
    18601 ggcagcatca ccgtagggcc gaggtcgaga atataacgca tgacttctga aaacataggt
    18661 cacccctgta agatagtcag aattttattt tgtaatgctt cgataccgac accagaaaca
    18721 aaaggcatgc cgtgaactaa cggaatatcg ccaaaactac ggtcaacttt ggcggtagta
    18781 catatcaaat gcacgccatc catataggtc tctatttcat taacccgaca ctggattaat
    18841 tcaacaggaa tattatgact ctgacacaac tctttaattt cttccgccgc catcgtagag
    18901 gtcgcaaccg cgcctccgca agcgacaata atcttgcgtt tcataaatag tcctttttat
    18961 tgttacagag ggatttattc gtctgaatct aaaacatact ttgtgaagta ttcctttaac
    19021 tgagtttcag gaagagtaat tagtgtctcg acgatctcgg gctgttgtaa cttgccaaat
    19081 aaacagcgta aaagtttcaa ttgctgctgc ggattttcca caatcaacgc aataaccaac
    19141 gataccgcca cgtcattatc atcatccgct tgctgaaaat gaactttatt tgttggcctt
    19201 aacagataaa tggctgacga cttagcatga atcgcctcac aatgcggtat tgcaatggcg
    19261 tgctgctcaa gcattatccc ggtagggaat tctgcttctc tggcaattaa tgcctgtggc
    19321 caggtatcat gaaccacacc tttcgcgagc atctcattac cgatatgggt taaaacttcg
    19381 ctacgatcga caaaagaaat tccgctacga acaaacaggt tagtcatata ccgtccttat
    19441 tccgcacagc cgtagcgata ggcgcgcaaa acatcataaa ttttatccat aatcaactga
    19501 tgcggtattg ctgataattc cccggactga atgcgttcaa attgtttagg aagatactga
    19561 ctaatcatgc ccagagggat ttccatgcct tccagattca ccatcatcgt ttcgacgcta
    19621 tttttaatcc gactatgcgg ccagtaataa cgaatacgat ccgacaggct gtaacgaata
    19681 tccagtaatg aatcgttaaa acccgggcga taatatttct tccagtactg cggttcgtcg
    19741 agcatcactt cttcaattac agccaggcaa ccgctgcgat tttcgggggc gataagttcc
    19801 tgctcaattt gtgccaacgc aaatatcgcc tcacgtaaag caaaggttaa tgcgggaccg
    19861 actttcaata ttgcaaagtg atcgcggact aattcccaat aagccgtccg ggtctggtaa
    19921 tcggtagaat gtgcttcata aaccattcgg gtgttttcta tccattgcgc cagcggctgt
    19981 gcttcctgcg gctgataatg gataatattg ctgtgatcaa attccacgcc tggctgcacc
    20041 acaatggcaa tcacgcgtgt taacgcctct gccagcccac gggcaataaa ggccttttga
    20101 tgcgtacgta aagtattggc ggcatcttca acatgggtga tgtgtactga ctgaatggcg
    20161 ctggcctcac cgcccggaac cggcacttcg gtgccaatga catagctcag ttgctcacgc
    20221 tggcaatctg tcgccacact ttccgcagca aagcaaagca cagcagctcg ttccgcaacc
    20281 gtttctggtg ctaacggtat gggatccccc gcgcaggaca ttgacgcatc aagatgaatt
    20341 ttactgaagc cggcacgaac atatgccttt accagctcga cggatttttc catcgccgca
    20401 tccgcatttt cttgctgcca gcagtttggc cccagatggt cgccgccgag aataatgcgt
    20461 tcgcgcgcaa acccaactct atcggcaatc gcaaaaacaa attcgcgaaa gtccgccggt
    20521 gtcatgccgg tataaccgcc aaattgattg acctggtttg acgttgcttc aatcagcact
    20581 ttgcgcgtgc tgttgcgatc aaatgccagc gccgc
//
LOCUS       DAYPHN010000077        20292 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000077 DAYPHN010000000
VERSION     DAYPHN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.077"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(112..597)
                     /gene="sixA"
                     /locus_tag="ACG0BW_002692"
     CDS             complement(112..597)
                     /gene="sixA"
                     /locus_tag="ACG0BW_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFK7259641.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(800..2944)
                     /gene="fadJ"
                     /locus_tag="ACG0BW_002693"
     CDS             complement(800..2944)
                     /gene="fadJ"
                     /locus_tag="ACG0BW_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFK7259642.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2944..4254)
                     /gene="fadI"
                     /locus_tag="ACG0BW_002694"
     CDS             complement(2944..4254)
                     /gene="fadI"
                     /locus_tag="ACG0BW_002694"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFK7259643.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4435..4719)
                     /gene="yfcZ"
                     /locus_tag="ACG0BW_002695"
     CDS             complement(4435..4719)
                     /gene="yfcZ"
                     /locus_tag="ACG0BW_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFK7259644.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5091..6431
                     /gene="fadL"
                     /locus_tag="ACG0BW_002696"
     CDS             5091..6431
                     /gene="fadL"
                     /locus_tag="ACG0BW_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFK7259645.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6798..7178
                     /locus_tag="ACG0BW_002697"
                     /pseudo
     CDS             6798..7178
                     /locus_tag="ACG0BW_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8224..8979)
                     /gene="mlaA"
                     /locus_tag="ACG0BW_002698"
     CDS             complement(8224..8979)
                     /gene="mlaA"
                     /locus_tag="ACG0BW_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFK7259646.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            9273..10205
                     /gene="yfdC"
                     /locus_tag="ACG0BW_002699"
     CDS             9273..10205
                     /gene="yfdC"
                     /locus_tag="ACG0BW_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFK7259647.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            10281..10355
                     /locus_tag="ACG0BW_002700"
     tRNA            10281..10355
                     /locus_tag="ACG0BW_002700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10314..10316,aa:Arg,seq:cct)
     gene            10535..10717
                     /locus_tag="ACG0BW_002701"
     CDS             10535..10717
                     /locus_tag="ACG0BW_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFK7259648.1"
                     /translation="MWRDSILHSAMRSLMNSQARDTRDKSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(10797..12044)
                     /locus_tag="ACG0BW_002702"
     CDS             complement(10797..12044)
                     /locus_tag="ACG0BW_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFK7259649.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKGHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(12106..13029)
                     /locus_tag="ACG0BW_002703"
     CDS             complement(12106..13029)
                     /locus_tag="ACG0BW_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFK7259650.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKDYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSSGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELESEK"
     gene            13245..14678
                     /gene="cscA"
                     /locus_tag="ACG0BW_002704"
     CDS             13245..14678
                     /gene="cscA"
                     /locus_tag="ACG0BW_002704"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFK7259651.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDSEGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWELKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDTLALRIFIDTSSVEVFINDGETVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(14686..15681)
                     /locus_tag="ACG0BW_002705"
     CDS             complement(14686..15681)
                     /locus_tag="ACG0BW_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFK7259652.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRNRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYCQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            15924..16142
                     /locus_tag="ACG0BW_002706"
                     /pseudo
     CDS             15924..16142
                     /locus_tag="ACG0BW_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            16160..17491
                     /gene="dsdA"
                     /locus_tag="ACG0BW_002707"
     CDS             16160..17491
                     /gene="dsdA"
                     /locus_tag="ACG0BW_002707"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFK7259653.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALQETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(17597..19135)
                     /gene="emrY"
                     /locus_tag="ACG0BW_002708"
     CDS             complement(17597..19135)
                     /gene="emrY"
                     /locus_tag="ACG0BW_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFK7259654.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(19135..>20292)
                     /gene="emrK"
                     /locus_tag="ACG0BW_002709"
     CDS             complement(19135..>20292)
                     /gene="emrK"
                     /locus_tag="ACG0BW_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFK7259655.1"
                     /translation="QINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTD
                     DAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANI
                     VRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLIS
                     SKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIKSPVTGYIA
                     QRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVF
                     HGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTA
                     TIDTRNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         5612 a         4993 c         4638 g         5049 t
ORIGIN      
        1 cattcgatca caagcggatc attgttagta caataagatt gcataaattc aacattgcca
       61 gaaaaaaata aaggcccgat agattacacc agcgggcctt gtgcatgcgt attaaatcgc
      121 ttttgccatc ttcagattac acgggctcat ctgccagttg aatgtgccgt ttccgctttc
      181 atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt cgcccggaca
      241 taactcagca accagatacc cgactaaggg taagtgggag atcaccagta ccgaagcaac
      301 accttcattg gtcaacgcct gtaaataggc actgaccaga ccaacatcgc cgcagggcgt
      361 taactccggc agaacttctg cactggaagg caggttcaga caatccccta cttcttccag
      421 tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca ctttttgacc
      481 tttcagccag ttcgccatca ggcgagattc gtcacaaccg ttagtagtca gaggacgaac
      541 cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa cttgcatatt
      601 gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag agaaagaaaa
      661 cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt tacctcaatg
      721 gcgtaagtat agtcaatatg cgtttacatt ttgagcaaca ctccgccatt cagcgcggat
      781 tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa aaactttccc
      841 cacgcttgct catctcgact aaacgctcgc aaggggtaaa acgggaacca tactgcgtgg
      901 ccagtcgttg cattattgca accacttcac ccgcaccgag agaatcgatg tagcggaacg
      961 gaccaccgag aaatggcgga aaaccaatgc caaataccgc gccaatatct ccgtcacgca
     1021 cgctacggat aacctgctcg tccagacaac gtactgcttc attcagcatc aacatcacac
     1081 accgttcagc tacctgcggt gcggagagtc gcccctgccc ttgtgtgcca atcagcgggt
     1141 aaatggcggg atcgacctgt tttttgcttt tacgcccttt ctgaccataa agatagaaac
     1201 cccggccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca acatttgcag
     1261 gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta gtcccggtgt
     1321 cgattcctac ctcatccaaa agttggattg ggcctaccgg aaaaccaaat ttcaccagcg
     1381 cggcatcaat gtgctctacc cgttcacctt cggtcaacat gcggatagct tcattaatgt
     1441 aaggcgctaa aatgcgattg acgtaaaacc cggctttgtc acgcacgaca atgggcgttt
     1501 taccctgttt tttcgccagt tttactgtgg tagcgatggt ttgcgccgat gtccccgcat
     1561 gaggaataat ctccaccagc ggcatttttt ccaccggact gaagaaatgc aggccaataa
     1621 cttgctcagg tcgcgtagcg tgagcggcga tatcaccaat cggtaaagat gacgtattcg
     1681 aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc cgccaccatt tgttgtttta
     1741 attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag ccgcgatagt
     1801 ccgtcgttcc ggagattaat gccagctgtc tgtcacgttc gctggctttg agatgacgac
     1861 ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt atgccctgcg
     1921 ggttgatatc tttaattctg accggaagcc ccgctttaca agcagtgacg taagcaatac
     1981 cgccgcccat caagccacca cctaaaattc ccacgctgtt taatggcgca ggcggcgcat
     2041 cactgccagg atctttcttc acttccgtac tggcaaaaaa gatactacgc agcgcctgcg
     2101 attgcggcgt catcgccagt tcgccaaaag cacgggcttc agcgtcatag ccgctactgg
     2161 tgccctgcgc taatcccgtt tcgacaacct ccaggatgcg ttctgtcgcc ggataattgc
     2221 cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca cgacctaacg
     2281 gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga tcctgctttg
     2341 ccagctcaac agcggcttcc agtaaaatgg attgcggaac gacgtcatcc accagcccca
     2401 gctttaatgc ctgtttcgcc cgaagttgtt ttccagtgag gatcatctcc aatgctgtgc
     2461 tgacacctat caggcgcggt aaacgctggg tgccgcctga accgggtaat aatccaagtt
     2521 gtacttcagg caaaccgagc accgttttag gatcgtcagt acacatgcga ccgtggcacg
     2581 ccagcgccaa ctccagccca ccgcccaggc aagcgccatg aatagccgcg ataaccggaa
     2641 cgggcaaagc atgaatctcc gccattaact gttgaccctg ccgcgccagt gcttccgctt
     2701 cttgcgccgt tttgcagttg ccgatcatgt tgatgtctgc gccagcaatg aagttgtccg
     2761 gtttagcgga gacaaacacc acgcctctca actctttgtt ttcacggagt tgcttaataa
     2821 tggcgcgcac ctgcgaggca aactccgcct tcagggtatt cattttctca cccggtacgt
     2881 cgatggtgat aacagcaatg ttgtccagac ggacattaag ggtaaacgct gatgccattt
     2941 ccattattcc gcctccacaa ccattgccgc gccaagccca cccgcagcac aggcggtcac
     3001 taatccaaat ccaccgccgc gacgccgaag ttcatgcaat gtctgggtaa tcatccgcgc
     3061 gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg ccaagcacgt taaatttgct
     3121 atcgtccact tcgccagtgg catgtgcacg ccccagtaca tcacgagcaa aacgttcgct
     3181 acccagcaac tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca tatcgatcaa
     3241 tgtcagatcg ctcattgtca aaccggcacg ttccagcgcc agcggcgttg accaggccgg
     3301 accgagcaac atgtcctgcc agacatctat cgcagtaaat gcgtaactgc gcagataccc
     3361 cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca ccgctgccgc
     3421 gccatcggtc agcggcgtac tgtttgccgc cgttaccgtc ccgtgtttac gatcaaacgc
     3481 cggacgcaac tttgcgtaat cagcaagcga ggaattaccg cgaatattgt tgtcttcggc
     3541 aagcggttgt ttataaggag ggataaaggc agtcatcacc tcttctttga gtttaccatc
     3601 tgaccacgcc tgagcggctc gctgatgcga acggtgcgct aatgcatctt gctgttcgcg
     3661 agtaataccg taggttttcg ccatttgttc agcggtgtcg cccatccgca agccagtaga
     3721 gtattccgcc actgccggag gcaccggcat taagtcgcgc aaacgcaggc gagagaagag
     3781 tttcaggcgc tggctcatgg ttcgagcttt gttgacatca accagcacgc gcgccagttt
     3841 tttactgacg ccaattggca atacggaaga ggaatcggcc ccaccggcaa tccccgcgcg
     3901 aatagttccc gccatcaggc tttctgcgac gtttgcaatc gcctggaaac tggtagcgca
     3961 agcgcggctg acgctgtaag catcggtatg cacattcatt ccggtgccga gaacaatttc
     4021 acgcgcaatg ttgggggctt ccggcatttg tacgacctga ccaaagacca gttgttcaat
     4081 cacttcggcg gggatctcgc tgcgtgccag caattcgcct accaccatct tccctaaatc
     4141 aaccgcggga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac gtaaaccgct
     4201 aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac ccataacact
     4261 cccctgtaaa aaaagatata aagtggtctg acctgatcat agtcttaacc atttttttac
     4321 atttagccaa gcggagaaaa aggaaagtgg gagctatgac acagagagaa aagtagaaga
     4381 gaaaataaaa cgcccccgcc aacaactgac aggggcgtac gtacccgaga ggaattaacg
     4441 cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat cgatatccag
     4501 gcgcacaccg tcagactctt cagtgaaagt cggggtgatt ttgcacggtt cggattccac
     4561 gctacgggct ttttcagtca gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa
     4621 cacgcggctg taagacgcgg tgcagtcgga gttgtccata atggtgccaa catccataca
     4681 gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc tctgtatgtg
     4741 cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt gctgctccag
     4801 ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg atctaaaaca
     4861 aaattcaccc gaatccatga gtgcgccacc tccaaatttt gccagctgga tcgcgtttct
     4921 tagatcatat ttgaaaaaag atagaaacat acttgcaaca ttccagctgg tccgacctat
     4981 actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc gcgctcctgt
     5041 tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc atgagccaga
     5101 aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc acccaggcct
     5161 ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg gcttattcag
     5221 gggaaggtgc aattgccgat gatgcaggta acgttagccg taaccccgca ttgattacca
     5281 tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgacccggat gtaaatatca
     5341 gcggaacgtc tccatctggt cgtagcctga aagccgataa catcgcgcct acggcatggg
     5401 ttccgaacat gcactttgtt gcaccgatta acgaccaatt tggttggggc gcttctatta
     5461 cctctaacta tggcctggca acagagttta acgatactta tgcaggcggc tctgtcgggg
     5521 gtacaaccga ccttgaaacc atgaacctga acttaagcgg tgcgtatcgc ttaaataatg
     5581 catggagctt tggtcttggt ttcaacgccg tctacgctcg cgcgaagatt gaacgtttcg
     5641 caggcgatct ggggcagctg gttgccggtc agatcatgca atctcctgcc gggcagactc
     5701 ctcaagggca agcgttggca gctaccgcca acggtatcga cagtaatacc aaaatcgctc
     5761 atctgaacgg taaccagtgg ggctttggct ggaacgccgg tatcctgtat gaactggata
     5821 aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa aattgacttc aaaggtaact
     5881 acagcagcga tcttaatcgt gcgtttaata actacggttt accaatacct accgcgacag
     5941 gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc tgaaatgtgg gaagtgtcgg
     6001 gttataaccg tgttgatccg cagtgggcaa ttcactatag cctggcttac accagttgga
     6061 gtcagttcca gcagctaaaa gcaacctcaa cgagtggcga cacgctgttc cagaaacatg
     6121 aaggctttaa agatgcttac cgcatcgcgt tgggtaccac ttattactac gatgataact
     6181 ggaccttccg taccggtatc gcctttgatg acagcccagt tccggcacag aatcgttcta
     6241 tctccattcc ggaccaggac cgtttctggc tgagtgcagg tacgacttac gcatttaata
     6301 aagatgcttc agtcgatgtt ggtgtttctt atatgcacgg tcagagcgtg aaaattaacg
     6361 aaggcccata ccagttcgag tctgaaggta aagcctggct gttcggtact aactttaact
     6421 acgcgttctg ataacgcgtt cgcctggata aagtcacctg ctatgcaggt gactttaact
     6481 cccacacttc aacgaaagtt gtttcctcat tgcttgcctc ctgagttttg caaacagcct
     6541 gatggtagct tgccctttca aatcattaag tggtatccac agacaaacct gaagggaaag
     6601 gcatattttc aggcgttctg ctcgtctttc tcaaagagtt tacttttctg aatttccagg
     6661 atactcaaac caccctggct attgtgcgct catacactca aattaaagat agggtctaaa
     6721 taaatgagcg ttttttgata gtctatttta ttaggtaata tatatttgta acaaatcaat
     6781 caacatggaa taaaatcatg ctaccatcta tttcaatcaa caataccagc gcagcttaca
     6841 cagaatccat caatgaaaat aacaatgatg aagttaatag attagtacaa gagctcaaaa
     6901 acctttttaa tggtaagcaa ggaataagca cctgtgttaa acatctactt gcgcttataa
     6961 aaaacgccat acgaggaaac gacaatcctg atagatttaa tattaataat tcctcagtta
     7021 cttatattga tattggctcc aatgatacag accacattac tattggtatc gataaccaag
     7081 aaacaataaa attacttgcg agctataaag acaaagaact cgtccgtgct atcattaatg
     7141 acaacatagt tgagaagact catgatgata taaataatgc tgactttact gatggtagta
     7201 tcgttgttag tggtaataag ggtgctattg actccaatga tacagccgat attactagtg
     7261 gtatctataa tcaagaacca ataaaatcac ctacgaacga tgaaaacaaa aaagtcggcc
     7321 aaaccatcat ccatgaaaat gtagtagaga atactcatga tatcaataac aaggaaatga
     7381 tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc gataagatat
     7441 cacaaaaact cagacatacg ataacggaat ttgatgagaa tgggaaaagc gaaccaacgg
     7501 acttatttac ctggtacggt aaagataaaa aaggcgattc tctcgctatt gtaattaaaa
     7561 ataaaaacgg aaatgattac ttatctctcg gttactatga tcaggacgac taccacattc
     7621 aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa aaagccagga
     7681 atgcttcagc gtggtttgaa agcagtaaag ctatcatggc agaatcattt gcaacaggtt
     7741 ccgatcatca ggttgtaaac gagctcaacg gggaaagact gagagagcca aacgaagttt
     7801 ttaaacgttt aggtcgagca ataagatata attttcaagt agacgatgca aaatttagac
     7861 gcgataatgt aaaagaaata atttctactt tagtcgataa cgaagttgac cgttctcaaa
     7921 ataaatatga ccactttaag gaaatcgaag ataaagttga aaaaaggctt caaaatcgcc
     7981 aggctaaata tcaaaatgaa attaatcaac tatctgcatt gggtgttaat tttgatgata
     8041 attaaaattg tccagttttt ttgatttttc aacgcctgaa tatcggaaat agtcagtgac
     8101 gaacatcagt atttttacat aattatgttc aggtcattgt cggtttatct tcttttactt
     8161 ggggtaattg agggataaca acaaaaaagg tgagttttgt gactcacctt ttttatttgt
     8221 ttcttattca gaatcgatat cttttaaatc atcctgaatc gcttgcgcgt tcggattttc
     8281 ctgcggtttg agttcgccgc cattagcgat gaaatcatga cgctggaagt acgcttcgcg
     8341 caccataata taaggatcgg acgactgacg cagcagacca tcggaatcca gcagctgtgc
     8401 gcgggtttcg atcccttcaa gcgtccattt acccacagac atcggccagg tcagccagga
     8461 aagaaccggg taaagaccat ccgccatatc accaccgtca tcacgcagcg tgaagctacc
     8521 gtagaacggt aactgaacgt atggcccata acccacacca taatgaccaa gcgtactacc
     8581 gaagcggtga ggttcagtcc gttgcagttt cgggttcgcc atccctgcaa catcaataaa
     8641 gccgcccatc cccaaaatgg tgttcaggaa aaagcgggta aagtggacca tcccctgata
     8701 agggtcgccc tgcaagaagt agttaaccat caccgcaggt tcttcaaggt tgccagtaaa
     8761 gttgctcaaa ccgttacgcg ccggttgcgg aacataatca cgccaggcga cagcgaccgg
     8821 tcgaacaata tacgggtcta atacattgaa gttgaagttg tacatggtgc ggttgaaccc
     8881 ttctaacggg tcagaacgcc cttgctgatc tgtaccggaa ctcgcacacc ccaccagaag
     8941 cgtagttccc agagcaagcg ccgacaggcg aagcttcata aatgtctccc tgttttttta
     9001 tggcttatgc agtttgccat ccatgacgga acgataccgt atccgcctgt ttaggtgtgg
     9061 gcgattgtaa cagcacgtca actgatgtcc agacgccctg atttgctgat ttgatcatag
     9121 cctggtaatc gccgccctgt aggctacttg attctataga aacagaaaaa ggcaaacgcg
     9181 gccttttcta cattttcaga gtaactcccg ccagttgcga gcaaaaaagc cgctacgctt
     9241 tagctatacg tgctaattca agagaagaga ccatggacaa cgacaaaatt gatcaacaca
     9301 gcgacgaaat tgaagttgag agcgaagaaa aagagcgcgg caaaaaaata gaaatagatg
     9361 aagatcgact cccctcccgg gcgatggcaa ttcatgagca tatccgccag gatggtgaaa
     9421 aagagctgga acgcgacgca atggcactac tgtggtcagc cattgcggcg ggtctgtcga
     9481 tgggcgcttc attactggca aaagggatat ttcatgtcga actggaaggt gtgccgggca
     9541 gcttcttgct ggagaatctc ggttatacct ttggttttat tatcgtcatt atggcccgcc
     9601 agcaattatt taccgaaaac accgtgactg cggtactacc cgtcatgcaa aaaccgacaa
     9661 tgagcaacgt tggcttactt atgcggttat ggggcgtcgt gctgctgggt aatattctcg
     9721 ggacaggtat tgcggcgtgg gcatttgaat atatgccaat cttcaatgaa gaaactcgcg
     9781 atgcatttgt caaaatcggc atggatgtga tgaagaacac ccccagcgag atgtttgcca
     9841 acgcgatcat ttccggctgg ctgatcgcca ctatggtatg gatgtttcct gcagcgggtg
     9901 cggcaaagat tgtggtgatt atattgatga cctggcttat tgccctgggt gacaccactc
     9961 acatcgttgt cggttctgtt gaaatcctct atctggtgtt taacggtacg ctgcactgga
    10021 gcgatttcat ctggcccttc gcactaccta ctttagcggg gaacatctgc ggcggcacct
    10081 ttatcttcgc gttaatgagt catgcacaga ttcgtaacga catgagcaac aagcgcaaag
    10141 cagaagcacg ccaaaaagca gaacgtgcgg aaaacattaa gaaaaatgat aaaaacccgg
    10201 cataaatggc gagggtttaa gcaatcgagc ggcagcgtac ttaccccgca gtccattagc
    10261 gggtatactc atgccgcatt gtcctcttag ttaaatggat ataacgagcc cctcctaagg
    10321 gctaattgca ggttcgattc ctgcagggga caccagatac ccttctaaca atatctacct
    10381 tcaccccgta aaagatgggt ttggcagcac acttgcccta tatctactca tttttactgc
    10441 aacaggttga aatctcagca ctgtcagaaa gcgctgatga ctaaacagcc ctgggccggg
    10501 cgatgtaacc atcatacaga atcctgatag cgaaatatgg cgtgactcga tacttcactc
    10561 tgcaatgcgt tccttgatga attcgcaggc ccgtgataca cgggacaagt cgctgaatga
    10621 cgacaatgtc ctggaaatca gcgaaccgcg tatccggagt acatttgagc gactgtacca
    10681 gaacatgaat gaggcgtttg gattaggtga ttattagttg ggctaagcat ttttgtatta
    10741 ttattttccg gttgagggat atggagatat cgacaacaac cggaaaaagt ttacgtctat
    10801 attgctgaag gtacaggcgt ttccataact atttgctcgc gttttttact caggaagaaa
    10861 atgccaaata gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag
    10921 cctgcgtggt caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg
    10981 gcaatttgaa aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa gtttgccacg
    11041 ctgtatttga agacggatat gacacaaagt ggaacctcaa tggcatgtaa cagcttcact
    11101 aatgaaataa tccaggggtt aacgaacaac gcgcaggaaa ggatacgcaa cgccataatc
    11161 acaacaccga taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc
    11221 gccatgcaca gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt
    11281 cctacatcgt gtgattcgaa taaacctgca taaaagacag gaaagagttg ttgatcaaaa
    11341 atgttataga aagaccacgt ccccacaata aatatgacaa aaacccagaa gtttcgatcc
    11401 ttgaaaactg cgataaaatc ctcttttttt acccctcccg catccgccgc tacacactgg
    11461 tgacccttat ctttaaaacg catgttgatc atcataaata cagcgccaaa tagcgagacc
    11521 agccagaagt tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata
    11581 gcatagccaa aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc
    11641 attttttcag tgaagctgtc aagcaaacca catcccgcca gataccccag gccaaaaaag
    11701 agcgccccca gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa
    11761 atcataaacg gtccggtcaa gaccagaatg aaactcatac accagatgag cggtttcttc
    11821 agaccgagtt tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg
    11881 ttgaccgaat aaagtgtacc taattccgtc cctgttaatc ctagatgtcc tttcagccaa
    11941 atagcgtata acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat
    12001 gcaaaacgat agtacgcatt tctgaatgga atattcagtg ccataattac ctgcccgtcg
    12061 ttaaaaaatt catgtcctat ttagagataa gagcggcctc gccgtttact tctcactttc
    12121 cagttcttgt cgacatggca gcgctgtcat tgcccctttc gctgttactg caagcgctcc
    12181 gcaacgttga gcgagatcga taattcgtcg catttctctc tcatctgtag ataatcccga
    12241 agaggacaga cctgtgagta acccggcaac gaacgcatct cccgccccag tgctatcgac
    12301 acaattcaca gacattccag caaaatggtg gacttgtcct cgataacaga ccaccacccc
    12361 ttctgcacct ttagtcacca acagcatggc gatctcataa tcttttgcca gggcgcatat
    12421 atcccgatcg ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac
    12481 gacatccgcc agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca
    12541 tagatcttca cgaatattgg gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt
    12601 catcgcagta aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg
    12661 taaccattcg ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact
    12721 ggggcggacc ataaacgtaa atgaacgttc tccttgatcg ttcagatcga caagcaccgt
    12781 ggatgtccgg tgccattcat cttgcttcag atacgtgata tcgacaccct cagttagcag
    12841 cgttctttgc attaacgcac caaaaggatc atcaccgacc cgacctataa acccacttgt
    12901 tccgcctaat ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag
    12961 tagccgcccg tctgattctg gcaagagatc tacgaccgca tcccctaaaa cccatacttt
    13021 ggctgacatt tttttccctt aaattcatct gacttacgca tagtgataaa cctctttttc
    13081 gcaaaatcgt catggattta ctaaaacatg catattcgat cacaaaacat catagttaac
    13141 gttaacattt gtgatattca tcgcatttat gaaagtaagt gactttattt ttataaaagt
    13201 taacgttaac aattcaccaa atttgcttaa ccaggatgat taaaatgacg caatctcgat
    13261 tgcatgcggc gcaaaacgca ctagcaaaac ttcacgagcg ccgaggtaac actttctatc
    13321 cccattttca cctcgcgcct cctgccgggt ggatgaacga tccaaacggc ctgatctggt
    13381 ttaacgatcg ttatcacgcg ttttatcaac atcacccgat gagcgaacac tgggggccaa
    13441 tgcactgggg acatgccacc agcgacgata tgatccactg gcagcatgag cctattgcgc
    13501 tagcgccagg agacgagaat gacaaagacg gatgtttttc aggtagtgct gtcgatgaca
    13561 atggtgtcct ctcacttatc tacaccggac acgtctggct cgatagtgaa ggtaatgacg
    13621 atgcaattcg cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac
    13681 agggtgtgat cctcactcca ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc
    13741 gtgaagccga cacatggtgg atggtagtcg gggcgaaaga cccaggcaac acggggcaga
    13801 tcctgcttta tcgcggcagt tcattgcgtg aatggacttt cgatcgcgta ctggcccacg
    13861 ctgatgcggg tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc
    13921 attatctgat gttttccccg cagggaatga atgccgaggg atacagttat cgaaatcgct
    13981 ttcaaagtgg cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc
    14041 attttactga acttgataac gggcatgact tttatgcacc acaaagcttt gtagcgaagg
    14101 atggtcggcg tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac
    14161 gtgaaggctg ggcaggctgc atgacgctgg cgcgcgagct atcagagagc aatggcaaac
    14221 tcctacaacg cccggtacac gaagctgagt cgttacgcca gcagcatcaa tctatctctc
    14281 cccgcacaat cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagt
    14341 tgcagtggga gctgaagaac agtgatgccg aacattacgg attacaactc ggcacaggaa
    14401 tgcggctgta tattgataac caatctgagc gacttgtttt gtggcgatat tacccacacg
    14461 agaatttaga cggctaccgt agtattcccc tcccgcaggg tgacacgctc gccctaagga
    14521 tatttatcga tacatcatcc gtggaagtat ttattaacga cggggaaacg gtgatgagta
    14581 gccgaatcta tccgcagcca gaagaacggg aactgtcgct ctatgcctcc cacggagtgg
    14641 ctgtgctgca acatggagca ctctggcaac tgggttaaca taatatcagg tggaacaacg
    14701 gatcaacagc gggcaaggga tccgcgtcac tcttccccct tcacgacctt caataatatg
    14761 caatgcagct tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa
    14821 aaacagatgc ccgacgccaa ccagattatc aaagcccatt acggcgacat cctgcgggat
    14881 acgtaccccc ttcgccagga gaacctgata agcgacaaag gctgcgcgat cgttaccaca
    14941 tatcagaaca tcaaaatctg gtttgccaga tttgaagtgg tcattgagta aacttgcgag
    15001 atcggtgtag tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg
    15061 tccagcatca cgccaggcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact
    15121 ttccggtagc cagaagcata acggttgaca atagcccgcc gcgagcaaat gctgtgttga
    15181 ttcatattgt gcagtgtaat catcagggat ataactgggt aacgctgggt catccgcaac
    15241 acagttcgcc aatacaatat tttcaccata cagagactca ggcagcgtga tatgtcgcag
    15301 ccccattgta gtatagataa tgccatccgg acggtgggca agcagctgac gtgccgcgcg
    15361 ggcagcgtca tcttcagaaa aaatattgat taaaaaacta ttccagccga actcgctggc
    15421 ggtttgctca atggcaagca gaatatcaac agagaaagga gtggtagccg tgtcctgcgc
    15481 cagcacggcg agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc
    15541 aggaacataa ttcagggtct ggattgcctg caatacgcgg ttacgcgttg caggacgcac
    15601 agattctgca ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc
    15661 gacatccttt aatgaagcca tacccaagcc gtttgccgta aaacgggcac tgtagcagaa
    15721 acagacgtca ctggcgagat ccaacgatat tctcgacgcc ctatcacccg acacggcaat
    15781 acaataaaaa ataacaataa ttcccggaca attgtaccca attccgcctc tgttctcgcc
    15841 aacgagtaac catgtaggca agtaggtaaa acatgccaac tggcatcgcc aatctggaga
    15901 agaaaatata aatatggatc attgtgctgg cgctgtatcc cgacatcagc ccggaaattt
    15961 ttacgattga tatcggttca agtgcaattg gctgcacgat cgttacggac tcgcttttct
    16021 ggctggtgaa gcaatattgc ggcgctacgc tcaatgaaac atttaaatac tatacgacag
    16081 cgacatttat cgcttcagtc gtcgctctgg cgggcacatt cctgctgtca tttatcatct
    16141 aagcgcaaag agacgtacta tggaaaacgc taaaatgaat tcgctcatcg cccagtatcc
    16201 gttggtaaag gatctggttg ctcttcaaga aaccacctgg tttaatcctg gcacgacctc
    16261 attggctgaa ggtttacctt atgttggcct gaccgaacag gatgttcagg acgcccatgc
    16321 gcgcttatcc cgttttgcac cctatctggc aaaagcattt cctgaaactg ctgctactgg
    16381 ggggattatt gaatcagaac tggttgccat tccggctatg caaaaacggc tggaaaagga
    16441 atatcaacaa ccgatcagcg ggcaactgct actgaaaaaa gatagccatt tgcccatttc
    16501 cggctccata aaagcacgcg gcgggattta tgaagtcctg gcacatgcag aaaaactggc
    16561 tctggaagcg gggttgctga cgcttgaaga tgactacagc aaactgcttt ctccggagtt
    16621 taaacagttc tttagccaat acagcattgc tgtgggctca accggaaatc tggggttatc
    16681 aatcggcatt atgagcgccc gcattggctt taaggtgaca gtacatatgt ctgctgatgc
    16741 ccgggcatgg aaaaaagcga aactgcgcag ccatggcgtt acggtcgtgg aatatgagca
    16801 agattatggt gttgcagtcg aggaaggacg taaagcagcg cagtctgacc cgaactgttt
    16861 ctttattgat gacgagaatt cccgcacgtt gttccttggg tattccgtcg caggccagcg
    16921 tcttaaagcg caatttgccg agcaaggtcg tatcgtcgat gctgataacc ctctgtttgt
    16981 ctatctgccg tgtggtgttg gcggtggtcc tggtggcgtc gcattcggac ttaaactggc
    17041 gtttggcgat catgttcact gcttttttgc cgaaccaacg cactcccctt gtatgttgtt
    17101 aggcgtccat actggattac acgatcagat ttctgttcag gatattggta tcgacaacct
    17161 taccgcagcg gatggccttg cagttggtcg cgcatcaggc tttgtcgggc gggcaatgga
    17221 gcgtctactg gatggcttct atacccttag cgatcaaacc atgtatgaca tgcttggctg
    17281 gctggcgcag gaagaaggca ttcgtcttga accttcggca ctggcgggta tggccgggcc
    17341 tcagcgcgtg tgcgcatcag taagttacca acagatgcac ggtttcagcg cagaacaact
    17401 gcgtaatgcc actcatctgg tgtgggcgac gggaggtgga atggtgccgg aagaagagat
    17461 ggagcaatat ctggcaaaag gccgttcata acgtttcaac gcagcatcgc catcctttcc
    17521 ctgggtgagc gatgctgccg atggcgcaga cttaagattc taccgtctta cctgctatag
    17581 ctctctcttt ttaccatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
    17641 ccaataccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
    17701 ttgaaagtga ctgctgagtt atttcattat taatttcatt aagaacccct gaaagcgaac
    17761 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgattg
    17821 ttgctgtcaa ctgactatgg tgtaatgatt ctcggcgtcc ccacagcgtc attgtcaacg
    17881 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
    17941 atttattatc tggcaaacct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcga
    18001 cggcgaatcc ctgaaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
    18061 atgtcacaga acgccagtaa tagcaaaccg catacattaa aaaactaaat gtcaccaaca
    18121 accgcatatc aattttgttg ccataacgtc ctatcaaagg tgatattaat agcggcatga
    18181 tgccgatggg cgcataggca agtccggccc atatcgcatt ataccccatc gtttcctgga
    18241 gtaactgcgg cataaggact atcgccccag agtaaaataa atacgcgcat gtgatactca
    18301 caataccaat ggtgaaatta cgggacttaa acaaactgag atcaagaatc gggttctctg
    18361 aggtcgactc ccaaatgact aaagaaatca gagaaataac tgacactact gttagtatta
    18421 ttattgtact cgagttgaac caatccaggt cgcgcccttt atcaagcatt atctgcaagc
    18481 caccaacacc gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagtct
    18541 cagtttctct tcctttaagt aaggttaagc ataatgtcag aacgataatc cccataggga
    18601 cattgattaa aaatatccaa ccccagctaa agttatcgca aatatagccg cccaatatcg
    18661 gcccacatat cggagcgata attacggtca ttgaccataa tgccaaagca aatgttcttt
    18721 tttctggcgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
    18781 ttaacccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aggctacaca
    18841 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taatcttaat tcgcctattc
    18901 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaagtga
    18961 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
    19021 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtga
    19081 cgcaccataa cgtcccaccg gttaatggtg ccggagttga tttagtgatt gccattaaag
    19141 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
    19201 taaagcctta ctggtataag caggcatgga cgtcacggtt gaagccagat cgggcatctc
    19261 ggcaatgtct tcgttcctgg tatcaatagt tgcagtcatc gataaaccaa tgcgcaaggg
    19321 atgttccatg agttcttttg gatcaagaga aacttcaacc gggacacgct gaacgatttt
    19381 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
    19441 attgatccct gtcacccgac catgaaacac aacattttca ccatagagat cgctgataat
    19501 attgaccgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
    19561 ccacatttga cgtgccggta caacagccat taacgattgt ccgggactca ctgtttcgcc
    19621 aacctgaacg cttctctggg caatatagcc ggtaacagga ctcttaatat ccgtacgttt
    19681 aagcactaac caggcttctt tggttgcatc cgccgcttca acgacttgtg gctgacggtt
    19741 taatggtgtg ttcattacta aagctttatt cgctttataa gcctgaatag cagcattcaa
    19801 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
    19861 cccctgcttc gctaaaggta cacgacggtt ataatcttct aaagattgtt gatactgaat
    19921 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
    19981 ccgaacaata ttggccagat tatttttagc tttattgagt gcgatagtgg catcagtctt
    20041 atccagtgag actaaaatgt caccctgtcg aacgtagttc gtatctttat gattaacgac
    20101 agtgacgctg cctgaaactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
    20161 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
    20221 aataaataaa actaccgcca ataaagaaaa gtattttctt ctgttagaat gttttttatt
    20281 tgaattaatc tg
//
LOCUS       DAYPHN010000078        20279 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000078 DAYPHN010000000
VERSION     DAYPHN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.078"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..759
                     /gene="bet"
                     /locus_tag="ACG0BW_002710"
     CDS             <1..759
                     /gene="bet"
                     /locus_tag="ACG0BW_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFK7259656.1"
                     /translation="GKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFIALLIVANQ
                     YGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESCTCRIYRK
                     DRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLAFGFAGI
                     YDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQI
                     FRRDIRASSELTQAEAVKALGFLKQKSTEQKVAA"
     gene            756..1436
                     /locus_tag="ACG0BW_002711"
     CDS             756..1436
                     /locus_tag="ACG0BW_002711"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFK7259657.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FAFGEQWR"
     gene            1433..1594
                     /locus_tag="ACG0BW_002712"
     CDS             1433..1594
                     /locus_tag="ACG0BW_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFK7259658.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGLYD"
     gene            1587..2144
                     /locus_tag="ACG0BW_002713"
     CDS             1587..2144
                     /locus_tag="ACG0BW_002713"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFK7259659.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            2221..2436
                     /locus_tag="ACG0BW_002714"
     CDS             2221..2436
                     /locus_tag="ACG0BW_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFK7259660.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2535..2753
                     /locus_tag="ACG0BW_002715"
     CDS             2535..2753
                     /locus_tag="ACG0BW_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFK7259661.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEEIELKNKQWGL"
     gene            2801..3079
                     /locus_tag="ACG0BW_002716"
     CDS             2801..3079
                     /locus_tag="ACG0BW_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFK7259662.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            3051..3401
                     /locus_tag="ACG0BW_002717"
     CDS             3051..3401
                     /locus_tag="ACG0BW_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7259663.1"
                     /translation="MESYSLTLDEACQFLKISRPTATNWIRTGRLQATRKDPTKPKSP
                     YLTTRQACIAALQSPLHTIKVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQSSMTS"
     gene            3323..4441
                     /locus_tag="ACG0BW_002718"
     CDS             3323..4441
                     /locus_tag="ACG0BW_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7259664.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDMTF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(4456..4531)
                     /locus_tag="ACG0BW_002719"
     tRNA            complement(4456..4531)
                     /locus_tag="ACG0BW_002719"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4496..4498),aa:Thr,seq:cgt)
     gene            complement(4646..5899)
                     /gene="proA"
                     /locus_tag="ACG0BW_002720"
     CDS             complement(4646..5899)
                     /gene="proA"
                     /locus_tag="ACG0BW_002720"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFK7259665.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(5911..7014)
                     /gene="proB"
                     /locus_tag="ACG0BW_002721"
     CDS             complement(5911..7014)
                     /gene="proB"
                     /locus_tag="ACG0BW_002721"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFK7259666.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7302..8357
                     /gene="phoE"
                     /locus_tag="ACG0BW_002722"
     CDS             7302..8357
                     /gene="phoE"
                     /locus_tag="ACG0BW_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFK7259667.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(8396..8797)
                     /gene="crl"
                     /locus_tag="ACG0BW_002723"
     CDS             complement(8396..8797)
                     /gene="crl"
                     /locus_tag="ACG0BW_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFK7259668.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(8855..10099)
                     /gene="frsA"
                     /locus_tag="ACG0BW_002724"
     CDS             complement(8855..10099)
                     /gene="frsA"
                     /locus_tag="ACG0BW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFK7259669.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSTADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(10191..10649)
                     /gene="gpt"
                     /locus_tag="ACG0BW_002725"
     CDS             complement(10191..10649)
                     /gene="gpt"
                     /locus_tag="ACG0BW_002725"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFK7259670.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            10910..12367
                     /gene="pepD"
                     /locus_tag="ACG0BW_002726"
     CDS             10910..12367
                     /gene="pepD"
                     /locus_tag="ACG0BW_002726"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFK7259671.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            12376..12657
                     /locus_tag="ACG0BW_002727"
     CDS             12376..12657
                     /locus_tag="ACG0BW_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259672.1"
                     /translation="MSTPAGVDYCLKPMAIYIEITKHYETHEKVAYLYSCDHISWGEF
                     LITKEGFALESVKELKGEKQKFYMFRAWGKVRRAYLTTGEFPQETCYAA"
     gene            complement(12674..13183)
                     /locus_tag="ACG0BW_002728"
     CDS             complement(12674..13183)
                     /locus_tag="ACG0BW_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFK7259673.1"
                     /translation="MFIGHLPAGYLITCTLMKRLPLIARHARWAMVIGLLGAVAPDFD
                     LFWCYLVDNGQRHHHLYPSHWPLLWFALLAATLVWAGIAKNKLPAWLGVVFCLNSIAH
                     LLLDTLVGDIWWLMPFVNQPFAFFHITAVHHPWWLNFLLHWSFLLELALVVAAIAMWC
                     RRNAKMVNA"
     gene            complement(13245..13859)
                     /gene="prfH"
                     /locus_tag="ACG0BW_002729"
     CDS             complement(13245..13859)
                     /gene="prfH"
                     /locus_tag="ACG0BW_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFK7259674.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAITVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFVAV"
     gene            complement(13856..14995)
                     /locus_tag="ACG0BW_002730"
     CDS             complement(13856..14995)
                     /locus_tag="ACG0BW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFK7259675.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDEIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDVLAFARINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKCTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(15186..15638)
                     /gene="yafP"
                     /locus_tag="ACG0BW_002731"
     CDS             complement(15186..15638)
                     /gene="yafP"
                     /locus_tag="ACG0BW_002731"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7259676.1"
                     /translation="MNNIHIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKDKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQCVECRGAWFTNFYMRYKPQH"
     gene            complement(15648..16046)
                     /gene="yafO"
                     /locus_tag="ACG0BW_002732"
                     /pseudo
     CDS             complement(15648..16046)
                     /gene="yafO"
                     /locus_tag="ACG0BW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414768.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
     gene            complement(16049..16342)
                     /gene="yafN"
                     /locus_tag="ACG0BW_002733"
     CDS             complement(16049..16342)
                     /gene="yafN"
                     /locus_tag="ACG0BW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFK7259677.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(16394..17449)
                     /gene="dinB"
                     /locus_tag="ACG0BW_002734"
     CDS             complement(16394..17449)
                     /gene="dinB"
                     /locus_tag="ACG0BW_002734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFK7259678.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKFCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(17520..18305)
                     /gene="lafU"
                     /locus_tag="ACG0BW_002735"
     CDS             complement(17520..18305)
                     /gene="lafU"
                     /locus_tag="ACG0BW_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFK7259679.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKIRPSH
                     PPKPATVAVPEETEKKAHDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            18277..19989
                     /locus_tag="ACG0BW_002736"
                     /pseudo
     CDS             18277..19989
                     /locus_tag="ACG0BW_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
BASE COUNT         5208 a         5221 c         5060 g         4790 t
ORIGIN      
        1 gggaagctgg ctgaacgagt cggcatggat tctgtcgacc cacaggaact gatcaccact
       61 cttcgccaga cggcatttaa aggtgatgcc agcgatgcgc agttcatcgc attgctgatc
      121 gtcgccaacc agtacggtct taatccgtgg acgaaagaaa tttacgcctt ccctgataag
      181 cagaacggca tcgttccggt ggtgggcgtt gatggctggt cccgcatcat caacgaaaac
      241 cagcagtttg acggcatgga ctttgaacag gataacgaat cctgcacatg ccggatttac
      301 cgcaaagacc gcaatcatcc gatctgcgtt accgagtgga tggatgaatg ccgccgcgaa
      361 ccattcaaaa cccgcgaagg cagagaaatc accggaccgt ggcagtcgca tcccaaacgg
      421 atgttacggc ataaagccat gattcagtgt gcccgtctgg ccttcggatt tgctggtatc
      481 tatgacaagg atgaagccga gcgcattgtc gaaaatactg catacactgc agaacgtcag
      541 ccggaacgcg acatcactcc ggttaacgat gaaaccatgc aggagattaa cactctgctg
      601 atcgccctgg ataaaacatg ggatgacgac ttattgccgc tctgttccca gatatttcgc
      661 cgcgacattc gcgcatcgtc agaactgaca caggccgaag cagtgaaagc tcttggattc
      721 ctgaaacaga aatccactga gcagaaggtg gcagcatgac accggacatt atcctgcagc
      781 gtaccgggat cgacgtgaga gctgtcgaac agggggatga tgcatggcac aaattacggc
      841 tcggcgtcat caccgcttca gaagttcaca acgtgatagc aaagccccga tcaggaaaga
      901 agtggcctga catgaaaatg tcctacttcc acaccctgct ggctgaggtt tgcaccggtg
      961 tggctccgga agttaacgct aaagcgctgg cctggggaaa acagtacgag aacgacgcca
     1021 gaaccctgtt tgagttcact tccggcgtaa atgttattga atccccgatc atctatcgcg
     1081 acgaaagtat gcgcaccgcc tgctctcccg atggtttatg cagtgacggc aatggccttg
     1141 agctgaaatg cccgtttacc tcccgggatt tcatgaagtt ccggctcggt ggtttcgagg
     1201 ccataaagtc ggcttacatg gcccaggtgc agtacagcat gtgggtgacg cgaaaagatg
     1261 cctggtactt tgccaactat gacccgcgta tgaagcgtga aggcctgcat tatgtcgtgg
     1321 ttgagcggga tgaaaagtac atggcgagtt ttgacgagat ggtgccggag ttcatcgaaa
     1381 aaatggacga ggcactagct gaaattggtt ttgcatttgg ggagcaatgg cgatgaagca
     1441 tcctcacgat aatatccggg taggcacgat cactttcgtc tactccgtta caaagcgagg
     1501 ctgggtattt cccggccttt ctgttatccg aaatcccctg aaagcacagc ggctggctga
     1561 ggagataaat aataaacggg ggctgtatga ctgatttcac cggaagcaat actcctgccg
     1621 aacatcgcga cagctggcgc acaccaccag agatttttgc tgcgcttaat gcagagttcg
     1681 tttttcaact tgatgctgcc gccagcgaaa aaaaccgact atgtcggctt tttatctcac
     1741 aggagcagaa cacattaacc acttcatggc ctgaagcaat gggatatgcc tctggttatg
     1801 tctggttgaa tccaccatac agcaatattt ccccttttgt gaaaaaggca gccactgaaa
     1861 acaaattcag tagtgtggga tgtgtaatgt tattgcctgc tgacacatct gtcggatggt
     1921 ttcatgaagc gatacaaacc gccagtgagg tcagattcat cacggcagga cgactggcat
     1981 ttattaaccc actcactgag aaacccgtca gtggaaataa taaaggctcg atgctcatta
     2041 tctggcaccc atacccccgt acacactgcc actttacgac cgttgatcgt ggagagttga
     2101 tggcgttcgg ctcaaggatt cttgcccgtc gggaggctgc atgacaacca cggaatgcat
     2161 ttttctggca gcgggcttca tattctgtgt gcttatgctt gccgacatgg gacttgttca
     2221 atgacacctc agcaggaaaa cgcccttcgc agcattgccc gtcaggctaa ttctgaaatc
     2281 aaaaaagcca gacagcagtt tccggataaa aacgtcgatg acatttgccg tagcgtgctg
     2341 aagaagcacc gcgaaacggt aacgctgatg ggattcacac cgactcattt aagcctggca
     2401 atcggcatgt taaacggcgt ctttaaggaa cgatgaacat gaaaagcaaa atcatcaggg
     2461 agctacaggc tcctttttta ttattcgcat tcaccctcaa gcgtattaac caacaattca
     2521 gggattaatg gaagatggca gacatcattg attcagcatc agaaattgaa gaattacagc
     2581 gcaacacagc aataaaaatg cgccgcctga accaccaggc tatatctgcc actcattgtt
     2641 gtgagtgtgg cgatccgata gatgaacgaa gacgcctggt cgttcagggt tgtcggactt
     2701 gtgcaagttg ccaggaggag atcgaactta agaacaaaca atggggattg tgatggcctc
     2761 aaagcagcaa atttcaacat cgtccaactg aggtgtaaaa atgttcagaa tcatttttcc
     2821 taacacctgg tacgtcgacc accacggcac tccctgcaaa atcctgcgtt ctacccacaa
     2881 caaagttcac tacatccgaa aaggcagaac atgtatcgcc agcatgttcc gctttaatca
     2941 tgactttgaa cctgtgaata aagctgatgc agatcggata gcagaagaga tcgaaacggc
     3001 agaacacatt aagaagttac gtgacatgcg ttcaaaaagc agaggtaatc atggaatcat
     3061 acagcctcac actcgatgag gcctgtcagt ttcttaagat atccagacca accgccacca
     3121 actggatacg aacaggccgc ctacaggcaa cacgcaaaga ccccactaaa ccaaaatctc
     3181 cttacctcac aacgcgacaa gcctgcattg cggcgcttca gtctccgctg catactatca
     3241 aggtgagcgc gggtgatggc ataacagagg aaagaaaatg tcactcttcc gcagaagtga
     3301 aatatggtac gccagtttca cattgccgaa cggtaaaaga tttaaacagt ctcttggaac
     3361 aaaggacaaa aggcaggcga cagagctcca tgacaagcta aaggctgaag catggcgggt
     3421 cagcaaactt ggtgaaatac ctgatatgac gtttgaggaa gcgtgtgtca ggtggctcga
     3481 agagaaagca cataagaaat cactggacga tgacaaaagc cggatcggat tctggcttca
     3541 acatttcgca gggatgcaac taagagacat cactgaatca aaaatttatt cagcaatgca
     3601 gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc agggcagaag catgcagaaa
     3661 aaaagggaaa cctgttccag aatacacgcc aaaaccagcg tccgttgcaa cgaaggctac
     3721 gcatctttca tttataaagg ccctactaag agccgcagag cgtgaatgga aaatgctgga
     3781 taaggcacca attattaaag tgcctcaacc aaagaataaa cggatccgct ggctggagcc
     3841 ccatgaagca caaaggctga ttgatgaatg tccggagcca ttaaagtctg ttgttgaatt
     3901 tgcactggca acaggcttaa gacgctcgaa catcatcaac cttgaatggc aacaaataga
     3961 tatgcagcgc cgggtggcat ggataaaccc ggaagagagt aaatcaaacc gcgcaattgg
     4021 cgttgcgctg aatgatactg catgtcgcgt attgaaaaaa caaatcggga atcatcaccg
     4081 ttgggtattt gtgtacaagg aaagctgtac caaaccagac ggaacgaaag cgccaacagt
     4141 caggaagatg cggtatgacg caaacacagc ctggaaagcg gcgctgagac gagctggtat
     4201 tgatgatttc agatttcatg acttgagaca cacctgggca agttggctgg ttcaagccgg
     4261 tgtcccgttg tcagtgttac aggaaatggg aggctgggag tctatcgaaa tggttcgtcg
     4321 atatgctcac ctcgcaccta atcaccttac cgaacacgca cggcaaatag actcgatcct
     4381 gaacccatcg gtcccaaatt tgtcccagtc aaaaaataag gaaggtacta atgatgtgta
     4441 acttattgat ttaaatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa
     4501 tgcgctgctc taccaactga gctatatcgg ccctgaaagg acatgttcac gaacgtgaat
     4561 cacggtggac aaggttaaaa ctaaccgggc gatgcgtcaa tggccttgtg aatcaaatgg
     4621 ctacttttgc atcacccggt tttatttacg cacgaatggt gtaatcacca atgccgatcc
     4681 acttgtaagt ggtcagtgct tccagcccca ttgggccacg cgcgtggagt ttttgtgtgc
     4741 ttaccgccac ttccgcaccc agtccaaact gaccgccgtc ggtaaaacgc gtagaggcgt
     4801 taacgtaaac agcggacgaa tccacttcgt taacaaaacg ctgggcgttg cgcatatcgc
     4861 gggtcaggat cgcatcggag tgttgtgtgc cgtgttcacg aatatgggcg atggcatcgt
     4921 caagatcgct gacgattttg acgttcaaat ctaatgacag aaactcatcg tcatactctt
     4981 ccgctttaac agccaccacc ttcgcggggc ctgtctgcaa ctgcgccagt gcacctgcat
     5041 cagcgtgtaa cgtcacgcca ctttccgcca tttgtttgct taatgcgggc aggaaactat
     5101 cggcgatgtt tttattcacc agcaacgttt caaccgtatt acatgtgctc ggtcgctgag
     5161 ttttcgcgtt gacgatcact tttaatgctt cagcgatctc cgcgctttca tcaacgtaaa
     5221 tatggcatac gcctatacca cctgtgatca ccgggatcgt cgactgttcg cggcacagtt
     5281 tatgcaggcc agcgccacca cgcgggatca gcatgtcgat gtatttatcc atacgcagca
     5341 tttcactgac cagcgcacgg tcaggattat caatcgcctg cacggcaccc gccggtaagc
     5401 cgcaggattt cagggcgtcc tgaatcaccg ccaccgttgc agcgttagtg cgacaggttt
     5461 ctttgccacc acgcaggatc actgcgttac cggttttcag gcacagcgaa gcgacatcaa
     5521 ccgtcacgtt cgggcgagct tcataaatca cgccaataac ccccagcggt acgcgacgac
     5581 gctcaagacg caggccgctg tccagtacgc caccatcaat cacctgcccc accggatcgg
     5641 cgaggttgca cacctggcgc acatcatcgg caatgccttt cagccgtgcg ggcgtcagtg
     5701 ccagacggtc aagcatcgct tcgctaaggc cattggcacg cgcgtcagca acatcctggg
     5761 cgttagcgtt gaggatgatt tcgctttgtg cttccagttc atcggcgatt ttttccagca
     5821 cgcgattttt ttcgcggctg gagagttgcg ctaatttata cgaggcttgc tttgcggcaa
     5881 tgcccatttg ttccagcatc ggccagctcc ttaacgggta atcatgtcat cacggtgaac
     5941 ggcaaccggg ccatattcat atcccagtat tgcatcaatt tcttgcgagt ggtgtccagc
     6001 aatacggcgt aatgcatcgc tgttgtaacg actgacgccg tgggcgatat cgcgaccttc
     6061 gaggttgcaa atgcggatga cttcaccacg cgagaaattg ccagtcacgc ttttaatacc
     6121 tttcggcaac agggagctgc cgcgttcaag aatggcggcg gttgcccctt catctaccgt
     6181 gatttcaccc gcaggtggtg caccgaaaat ccagcgttta cggttttcaa gcggggtcgc
     6241 ctgggcatga aacagggtac cgacggaaat gccttccatc acatcaccaa taacgcccgg
     6301 cttgctgccc gcggcaataa tggtgtcgat acccgcacgg caagccacgt ccgcggcctg
     6361 caatttggta ctcatgccgc cagttccgag gcctgaaacg ctgtcgccag caatcgcgcg
     6421 cagtgcgtca tcaatgccgt aaacatcttt aatcagttct gcctgcggat tgctgcgcgg
     6481 gtcagcggta tacaaacctt tttgatcggt cagcagcaac agtttatcgg cacccgccag
     6541 aatcgccgcc agcgcagaaa ggttatcgtt atcgccgacc ttaatctctg cagtagcgac
     6601 agcatcgttc tcattgatta ccggaacgat attgttatcg agcaacgctc gcagggtgtc
     6661 gcgggcgttc aggaagcgtt cacggtcttc catatcagca cgggtcagca gcatttgccc
     6721 gacgtgaatg ccataaatcg aaaacagctg ttcccacagt tgaatcagtc gactctgccc
     6781 taccgccgcc agcagttgtt tcgaggcgat agtcgctggc agttccgggt aacctaggtg
     6841 ctcacgtccg gcggcgatcg cgcccgacgt cacaataaca atccgatgcc cggcggcatg
     6901 taactgcgcg cactggcgaa caagttcaac gatatgggca cggttcagac ggcgcgatcc
     6961 gcctgttagc acactggtgc cgagttttac caccagcgtc tggctgtcac tcatgattct
     7021 ctgccattca attttaggaa aaatgatatc aaacgaacgt tttagcagga ctgtcgtcgg
     7081 ttgccaacca tctgcgagca aagcatggcg ttttgttgcg cgggatcagc aagcgtagcg
     7141 gcagttgttt acgcttttat tacagattta ataaattacc acattttaag aatattatta
     7201 atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca acgggactct
     7261 cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag agcactctgg
     7321 cattagtggt gatgggcatt gtggcatctg catccgtaca ggccgcagaa atatataaca
     7381 aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat atgagtgata
     7441 acgacagtaa agatggcgac cagagttata tccgttttgg ttttaaaggc gaaacacaaa
     7501 ttaacgatca actgactggt tatggtcgtt gggaagcaga gtttgccggt aataaagcgg
     7561 agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa tataaagatt
     7621 tgggttcttt cgattatggt cgtaacctgg gcgcgctgta tgacgtggaa gcctggaccg
     7681 atatgttccc ggaatttggt ggcgactcct cggcgcagac cgacaacttt atgaccaaac
     7741 gcgccagcgg tctggccact taccgtaaca ctgacttctt cggcgttatc gatggcctga
     7801 acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag caaaacggcg
     7861 atggcttcgg tacgtcattg acatatgact ttggtggcag cgatttcgcc atcagtgggg
     7921 cctataccaa ctccgatcgc actaacgagc agaacctgca aagccgtggc acaggcaagc
     7981 gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat ctggcaactt
     8041 tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat aagacacaga
     8101 actttgaagc ggtcgcccaa taccagtttg actttggtct gcgtccgtcg ctgggttatg
     8161 tcttatcgaa agggaaagat attgaaggca tcggtgatga agatctggtc aattatatcg
     8221 atgttggtgc tacgtattat ttcaacaaaa atatgtcggc atttgttgat tataaaataa
     8281 accaactgga tagcgataat aagctgaaca tcaataatga tgatattgtc gcggttggca
     8341 tgacctatca gttttaatgg atattgccgg atgtgatgca tccggcacat ttcactcacg
     8401 ccgttaactt caccggttca tcacgaaaat catccgccgg ttccagcttc agattcagcg
     8461 tcgccagcag ctcacgcagc ttctcgtgaa attcacgcag ggtgtgctcc agtcgttcaa
     8521 ccacttcggc atcttttatc tgaacactcg tccagtcgcc tgctttatcg aacagaccaa
     8581 actggtaact gtaggtaaaa cgggattcct gcgcttccag ttccatccac cagccccaga
     8641 actcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga
     8701 atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta aattttttga
     8761 tcaatctgct cttcgggtgt ccactcggta acgtcattgc aatctcctgt tgtgatgcaa
     8821 ctgttttacc aaattggcaa aatttagcaa atttttaaca caatcgtttt tcgatccagt
     8881 cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat gggatctcta
     8941 acaatttacc gtcagcagtt gatgaggtga ttaagcgtga gtcctcttcc gggctgaacg
     9001 gatcgttctt ccagtagcct gataacattg gcgttgggca acgacgtccc agcaatcctt
     9061 gcacttttaa tgaatagcga ttcaactcca cgcgtaacgc ttcgtcggaa gcatcatgca
     9121 tacccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc tggcacttaa
     9181 aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc agacgcggcg
     9241 attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca aaggccgcga
     9301 cgcgagtgtg atctacccac ggtacgttag gcagcgcctt taagacgtgc tgatgcaaca
     9361 ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc atatcaatag
     9421 tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa tagtccgtct
     9481 gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg cctttcggca
     9541 tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc tgccgcatgg
     9601 tgcccggcag acgctgagcg gcctcttcat aggcgcggtt tgacaaagcc tgcgcttgct
     9661 cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac gtagccgcat
     9721 gcagccagtg acgaccactg agttgcggat cttcttccgc acaggctttt tgttgccaca
     9781 ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca atcaccgtat
     9841 cgtataaatc atcgtcggta cgttcggcat cgctcatcac aatacgtgcc tggacgtcga
     9901 ggatttcacg tgggtcaatg ccgcgccaga tccacatcag acggttaatc atgcgatacc
     9961 agtgagggat ggttttacca tcaagggccg actgcacagg cggttgtgcg ccgtgattaa
    10021 agcggcgaac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac agggtttcgc
    10081 tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac tattgtaacc
    10141 tgcctgaagt taaaaagaac aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg
    10201 gagattggcg ggacgaatac gacgcccata tcccacggtt gttcgatcca ggtatcttgc
    10261 gggatatcaa caacataatc atcaaccagc ggacgaccag ccggttttgc gaagatggtg
    10321 acaaagtgcg cttttggata catttcacga atcgcaaccg cagtgccacc ggtatccacc
    10381 aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgttttag cactttaagc
    10441 tcgcgctggt tgtcgtgatc atagctggaa atacaaacgg tatcgacatg acgaataccc
    10501 agttcacgcg ccagtaacgc acccggtacc agaccgccac ggcttacggc aataatgcct
    10561 ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat ctgcaacatg
    10621 tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt atctacggct
    10681 taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg cgcactaaaa
    10741 accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga cgcgggataa
    10801 agtggtattc tcaaacatat ctcgcaagcc tgtcttgtgt tgacaacatt ttctgctaac
    10861 cctgtgacct gcaatactgt tttgcgggtg atcgacaagg agacttaacg tgtctgaact
    10921 gtctcaatta tctccacagc cgctgtggga tatttttgcc aaaatctgtt ctattcctca
    10981 cccgtcctat catgaagagc aactcgctga atacattgtt ggttgggcaa aagagaaagg
    11041 tttccatgtc gaacgcgatc aggtaggtaa tatcctgatt cgtaaacctg ctaccgcagg
    11101 tatggaaaat cgtaaaccgg tcgtcttgca ggcccacctc gatatggtgc cgcagaaaaa
    11161 taacgacacc gtgcatgact tcactaaaga tcctatccag ccttatattg atggcgaatg
    11221 ggttaaagcg cgcggcacca cgctgggtgc ggataacggc attggtatgg cgtctgcgct
    11281 ggcggttctg gctgacgaaa acgtggttca cggcccgctg gaagtgctgc tgaccatgac
    11341 cgaagaagcc ggtatggatg gtgcttttgg cttacagagc aactggttgc aggctgatat
    11401 tctgattaac accgactccg aagaggaagg tgaaatctac atgggttgcg cggggggtat
    11461 cgacttcacc tccaacctgc atttagatcg tgaagcagtt ccagctggtt ttgaaacctt
    11521 caagttaacc ttaaaaggtc taaaaggcgg tcactccggc ggtgaaatcc acgttggcct
    11581 gggtaatgcc aacaaactgc tggtgcgctt cctggcgggt catgcagaag agctggacct
    11641 gcgccttatc gatttcaacg gtggcacact gcgtaacgcc atcccgcgtg aagcctttgc
    11701 gaccattgct gtcgcagctg ataaagtcga cgccctgaaa tctctggtga atacctatca
    11761 ggaaatcctg aaaaacgagc tggcagagaa agagaaaaat ctggcattgc tgctggactc
    11821 tgttgcgaac gataaagctg ccctgattgc gaaatctcgc gataccttta ttcgtctgct
    11881 gaacgccacc ccgaacggtg tgatccgcaa ttccgatgtg gcaaaaggtg tggtggaaac
    11941 ctccctgaac gtcggtgtgg tgaccatgac tgacaataac gtagaaattc actgcctgat
    12001 ccgttctctg atcgacagcg gtaaagacta cgtggtgagc atgctggatt cgctgggtaa
    12061 actggctggc gcgaaaaccg aagcgaaagg cgcatatcct ggctggcagc cggacgctaa
    12121 ttctccggtg atgcatctgg tacgtgaaac ttatcagcgt ctgttcaaca agacgccgaa
    12181 catccagatt atccacgcgg gcctggaatg tggtctgttc aaaaaaccgt atccggaaat
    12241 ggacatggtt tctatcgggc caaccatcac cggtccacac tctccggatg agcaagttca
    12301 catcgaaagc gtaggtcatt actggacact actgactgaa ctgctgaaag aaattccggc
    12361 gaagtaatta caataatgtc cacgcctgcg ggcgtggact actgtttgaa gccaatggct
    12421 atctatatcg aaataacaaa acattatgaa actcatgaaa aggtcgcata tttatatagc
    12481 tgcgaccata tttcatgggg agaatttctg atcaccaaag aaggttttgc gctggaatcc
    12541 gtgaaagaac ttaagggtga aaagcagaaa ttttatatgt tcagagcctg gggcaaagtc
    12601 aggagagcat acttaaccac gggagaattt ccccaggaaa catgttacgc cgcttaaagt
    12661 cagaataatt ttttcatgca tttaccattt tggcattacg cctgcaccac atggcaatgg
    12721 cggcaaccac taatgccagt tccagtaaaa acgaccagtg taataaaaaa ttgagccacc
    12781 acggatgatg taccgccgta atatgaaaga atgcaaacgg ttggttaacg aaaggcatca
    12841 gccaccagat atcaccgacc agcgtatcga gtaataaatg cgcgatacta ttcaggcaaa
    12901 acaccacgcc cagccacgcc ggaagtttat tcttcgcaat tcctgcccag actaatgtcg
    12961 ccgccagcag agcaaaccat aataatggcc aatgagacgg gtaaagatga tgatgtcgtt
    13021 gcccgttatc aaccagatag caccaaaaaa gatcaaaatc gggcgcaacg gccccaagta
    13081 agccaataac catcgcccag cgcgcatgac gagcaatcag tggcaagcgt ttcattaaag
    13141 tacaagttat taaataacca gccggaagat gaccgataaa catgactatt cctttcgttt
    13201 ttcccgccgc tttcatatag aaaacggcgg gagacattcc tcaattacac agcgacaaaa
    13261 gccatccctg taaacgttcg tcgcgggttg ccacgttcaa tctggtgatg gaacattcgc
    13321 cgctgcgatt tcagcgccgc gctattttcc tgttgctgtt gctccagctt ccaggcaatc
    13381 agcaatcgcg ccagccgttt gttggcatgt tggctgcgct ctgactgaac cttcacgcta
    13441 atcccggatg ccagatgcgt ggcgcgtacc gccgagtcgg ttttattgac atgttgaccg
    13501 cccggccccg acgaacgcag cgtctcataa cggattgcat ccgattgttc ctgctcatca
    13561 gcagtaaaac gcccaatgcc cagaaaccag tttttgcgcc catgatgagg ccgatacgga
    13621 ctcggacaaa tccactggat agtgccgcac caactttcgc ttaatgccca tgcgttatcg
    13681 ccatccagag aaatcagcgc tgaacgcaac gtgtcagagt agcgacccgt ttctgtttcc
    13741 agcaccgtta tcgcgacgtc ttgtcgggta gcttctttaa tcaacctgtc cagcgccttt
    13801 ttcactgcga ggcaacattc ttccggcccc tgcgctgagg agagttgtag caagatcatc
    13861 cttttttccc tccactgttt ttgagcgtta gcaccggacg cagccgcgcg acaggaataa
    13921 ttaaaccggc ttgcaccaga cattgcacca cgctttcagc cgatttatag gcttgtggcg
    13981 cttcttcaaa gatgagttgt ttatcgcgac aaattacccg actgccaagt tcagtacgcg
    14041 agagttgcgt cgccgtgcat ttcgctgcca gacgcccttt acactcggtg cgcccccatt
    14101 tacgccctgc cccatgcgcc agcgagtgga gcgttttttc gttcgcaaca ggctgtacaa
    14161 gccaggagta atcaccgcgt gaaccgggaa taatcaccag accgttgtca tccggcgtgg
    14221 cacctttacg atgcaaccaa ccctgttgat cgccgatttg gcacgcgcta acaaagttat
    14281 gcgccacatc cagaaccgga cttccggtgg ccttaacttg ttgcattatg cgcaaagcaa
    14341 tcatctggcg attaatacgc gcaaacgcta gcacatcgtc atgttccgca atataccgta
    14401 gcgcgtcgtc actgccttca ggcaaaccat gatgcgaaaa cgaggcaata tgccgctgta
    14461 aaatagactg ccccaacccc ctcgagccgc tatgaaccag cagttgcaga tgctgcgcat
    14521 ccagacctgc cagtgcaaac agttcagcgt cgatgatttc atcaacttgt tgcagttctg
    14581 cgaagtggtt tccgccaccg atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg
    14641 ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaaa gctgataatc
    14701 gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc catagcgcca
    14761 tgccgcagcc gatatcattg ccgaccagtg ccgggtaaaa acggcctgcg gagaagaacg
    14821 ctgcgccaat cgggtagccg cgtccgggat gtaaatctgg catccctact acgcgctgca
    14881 tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt
    14941 catcagatgc gatggtaaat accgcatcgg ataagggacg aatatatttg cccataaacc
    15001 attccattta tgaataaaaa tcaggaaatg gcagacgaat ccagggcaag aaaaaaagaa
    15061 ttaatcctgg aaaggtctgc aaagagtcat tgataaagga tttgtcatga tttacctcct
    15121 tgcagtttgt ggaataagaa aacgcgcaca tactaggcgg gcgttatttt cattgcaagc
    15181 tggatttaat gttgcggttt atatcgcata taaaaattag taaaccacgc tccccggcat
    15241 tcaacgcact gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg ctttgcggtt
    15301 atgctggcgt ccaccgtaag ttcggattca gacttaatca aaggttttaa caaagcgctg
    15361 gcaaccccac ggcgggtgta ttcagggtca acaaataaca tatcgatata atgttcaatg
    15421 cgggaaataa aaccaaccgg ttgtgcatta atgactgcaa cccgcacttg tgatttcgcg
    15481 agtttatctt tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg ctgtggagaa
    15541 tagtgctgac tggcagtcat cgtaaccgct cgaaggaaaa tagcgcatag ttgctgaaaa
    15601 tcgccaggct gatagtttct tatatgtatg ttattcataa tataaattca aaaacgcatg
    15661 cgaaacgctt ctgccatttt cccaatttta tgcatttggt tgttatctcg agccagttta
    15721 tgaggttcag gtttcagaat ggcaatgagc aaccatgctt gctcatcaaa cgcaccctga
    15781 caatatacca aatgcgcttc gtcattcgtt ctgctgaatt ggcgcaactg tggcggaaat
    15841 ggattattct aatttgccag atgaatatga gcaactcgct caaatttgat taatggccag
    15901 gtaaacgagt cgtcgtagag tgcatcgcga ccaaatatat ctggcaaaac accgtcacgc
    15961 ttataggaaa taaaatccgc cgttaacgct tcaagttcct ctgctgtaag ttgcaggcga
    16021 atgagttttg ttttgaatac ccgcatcctt attccttaaa gtcattgaaa tcatcatccg
    16081 tcatatcatt aagatatttt tcagctcggc gtgtaatttc ctctaatctt gcagcacttg
    16141 gacggaagcg cgcctttata ggctttttct cctcttgttc agcaagcatg tccattaacg
    16201 cttcgaatgc gctggcactt aagagatatc ctgcggggcg attattagaa agaaccgcaa
    16261 ctggttgatc aataaagtat ttagctgggt ttttacgtaa ctcagtgata ttgaccgatt
    16321 tttccgcaag aattcgatgc atacagtgat cccctcataa taatgcacac cagaatagac
    16381 ataatagtat acatcataat cccagcacca gttgtctttc catttgaggg tcaagcaacg
    16441 tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg gcgttcatcc caggttttac
    16501 gcgcggtggc gattagatca gctttattca gccgtggcca gacgtgctcc tgagtggttt
    16561 gctggaaatc gtcgaacttt aatttcactc cctgacgggc aatcagtaaa tcaggtttta
    16621 cctttgccag acggcgttca agttccgggt acagccgctc gataatcgct tcacattcag
    16681 accagtgatg aatatcttcc gccatcgtgc gttccacgcc gacggatttt cgcaaccgtt
    16741 cgctgttaac gtcgcgctcg tcaatcccct gactacgctc ccacaaaatg cggccaaatt
    16801 tgccaaaacg tttgagcaga gtcaccagat cacacttttg cacatcaccg caggtccgca
    16861 gccccatcgc ttccagtttt gccgccgaga ctttgccgac gccaggaatt tttgccagcg
    16921 gtaaggtttg cagaaatgcc ggaacttctg ccggagtaat cacaaactga ccgttgggtt
    16981 tattcatgtc ggaagcgatt ttagcgagaa actttaccgg cgcaacgccc gcagacgccg
    17041 tcagttgcag ctcgttgaag attgtctggc ggatttcctg ggcgatgagg gtcgcagaac
    17101 cgtggcaatg cacactatcg gtgacgtcga gataggcttc atccagtgat aacggttcaa
    17161 tgcgcgaggt atagcgcgag aaaatttcgc ggatatgatt tgaggcttct ttgtaggcgt
    17221 caaagcgccc tggaagcagc gtgagatgtg ggcaaaattt gagcgccatc cctgtcggca
    17281 tagcgctacg tacgccaaat ttacgcgcgg gataattggc ggtgctgatc accccccgac
    17341 gttcgcggct gccgccaata gcaataggga tatcgcgcag ggcaggattg tcgcgcatct
    17401 ccaccgctgc gaaaaagcag tccatatcca catgaatgat tttacgcatt gctcacctct
    17461 caacactggt aaagtataca gtgatttcag agtttgagaa atgcataaag attcagcatt
    17521 taacgcgttc gctgcgaaag cacctgctgc ttatccagct tttgcaccag cggttgcacc
    17581 actttatccc catgctgacc aaagtattga tacagcgtat cggacgcact tttggtcagc
    17641 accataatct caatgcgccg gttgcccgcg ctttgcggat ttttggcatc cagcagcatc
    17701 tggtccgcca ttgcacttac ctgcatcact ttatcttccg gcattccggc ctcttccagc
    17761 acccgacgag ctgaaagcgc gcgatcgccc gaaaggttcc agttgttgta gatattgttt
    17821 ttgtaggcca tcgcatcggt atgcccggtg atgatgatct tattgtcgag cgaatcaaaa
    17881 accggtgcta actccaccag caaagtttta aagaacggca taatctgcgc gctgccgcgt
    17941 tcaaacatat tgcggttctg gtcatcttta atcagcacgc gcagtccctg gggaacaatc
    18001 tccatctcca gattcgcttc catatgcgca tcacgggcaa tggtgttgat actggttgcc
    18061 agctcgccaa gctcagtagc ggatttcttc ttcagcagcg ccgttttctc gttgacgtca
    18121 tgcgcttttt tctccgtctc ttccggcacc gccacggtgg caggttttgg cggatgagac
    18181 ggtctgattt tatttaacgg cgacagcccg ccaccgttaa aaatcgactg cccgtgcagc
    18241 gcggcgataa tgctttcacg ctcggattta ctgacgctgt tgacaatcaa ctttatcgtc
    18301 gtcaccaaag gggccgagcg tatttccgag gtttctgccc gctttacctt agacgcgatg
    18361 cccggtaaac agatggcgat tgacgccgat cttaacgctg ggttgatcaa ccaggcgcag
    18421 gcgcaaaccc ggcgtaaaga tgttgccagc gaggccgatt tctacggcgc gatggacggg
    18481 gcatcgaagt ttgtgcgcgg ggacgccatc gccgggatga tgattctggc gatcaacctg
    18541 atcggcggcg tctgtatcgg catcttcaaa tacaacctga gcgccgacgc cgccttccag
    18601 cagtatgtgc tgatgaccat cggcgacggg ctggtggcgc agatcccgtc cctgctgctc
    18661 tccaccgcag cggcaattat cgtcacccgc gtcagcgaca acggcgatat cgcccatgac
    18721 gtgcgccacc agctgctggc aagcccgtcg gtgctctaca ccgccaccgg gatcatgttt
    18781 gtgctggcgg tggtgccggg gatgccgcat ctgccatttt tgctgttcag cgccctgctt
    18841 ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg cggaggcgga ggagaaaagc
    18901 ctcgaaacgc tgacccgaac catcaccgaa accagcgagc aacaggtcag ttgggaaacc
    18961 attccgctga tcgagcccat cagcttaagc ctcggctaca agctggtggc gctagtcgat
    19021 aaagcccaag gtaacccgct cacccagcgg attcgcggcg tgcggcaggt gatttccgac
    19081 ggcaacggcg tgctgctgcc ggagatccgt attcgggaaa acttccgcct caagcccagc
    19141 cagtacgcca ttttcatcaa tggcattaag gctgatgaag cggatattcc ggcggataaa
    19201 ctgatggccc tgccctccag cgaaacctac ggcgagattg acggcgtgct ggggaacgac
    19261 ccggcgtacg ggatgccggt gacctggatc cagcccgcgc agaaagcgaa ggcgctgaat
    19321 atggggtatc aggtgatcga cagcgccagc gtgattgcca cccatgtgaa taagattgtg
    19381 cgcagctata ttcctgattt atttaactat gacgatatta cgcagttgca taatcgtttg
    19441 tcgtcgatgg caccgcgact ggcggaagat ttgagcgcag cgctcaatta cagccagttg
    19501 ctgaaagtgt accgtgcatt actgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc
    19561 gccactgtgc tggtcgccag tagcacattg accaaagatc atattctgct ggcggccgat
    19621 gtgcgcctgg cgctgcggcg cagcattacc catccgttcg ttcgcaaaca ggagctgacg
    19681 gtgtatacgc tgaataatga gctggaaaat ctgctgacca atgtggtgaa tcaggcgcaa
    19741 caggccggca aagtgatgct cgacagcgtg ccggttgacc cgaatatgct caaccagttc
    19801 cagagtacga tgccgcaggt gaaggagcag atgaaagcag cggggaaagc ccctgtgctg
    19861 ctggtgccgc cgcagctgcg tcctttgctg gcgcgttatg caaggttgtt tgcgccgggt
    19921 ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg agttgaagat tatgggggcg
    19981 ttgagttgat ggcctttggg gcgaatccgg catcggtgca caattgccgg atgcggcgtg
    20041 aacgccttat ccggcctacc gatccggcac aagtttgtag gcatgataag acgcgtcagc
    20101 gtcgcatcag gcatctgcgc acaattgccg gatgcggtgt gaacgcctta tccagcccac
    20161 cgatccggca caggtttgta ggcatgataa gacgcgccag cgtcgcatca ggcaccggtg
    20221 cacaattgcc ggatgcggtg tgaacgcctt atccggccca ccgatccggc acaggtttg
//
LOCUS       DAYPHN010000079        20247 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000079 DAYPHN010000000
VERSION     DAYPHN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.079"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            70..1104
                     /gene="fnlA"
                     /locus_tag="ACG0BW_002737"
     CDS             70..1104
                     /gene="fnlA"
                     /locus_tag="ACG0BW_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HFK7259680.1"
                     /translation="MFSSKTLLITGGTGSFGNAVLNRFLDTDIAEIRIFSRDEKKQDD
                     MRKKYNNQKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPMEAVKTNIL
                     GTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDRNKTVIC
                     GTRYGNVMASRGSVIPLFVDLIRAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIDTLAIALKELLNVPDHPVNVIGTRHGEKLYEALLSREEMIAAIDM
                     GDYYRVPPDLRDLNYGKYVEQGDSRISEIEDYNSHNTQRLDVEGMKELLLKLAFIRAI
                     RAGEKYNLDS"
     gene            1106..2209
                     /locus_tag="ACG0BW_002738"
     CDS             1106..2209
                     /locus_tag="ACG0BW_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFK7259681.1"
                     /translation="MKILVTGANGFIGRNLCLRLEELGYKDLIRIDRESTKQDLEQGL
                     QDADFIYHLAGINRPKTDDEFISGNSDLTKHIVEYLLSIGKNTPIMLSSSIQAELNNA
                     YGVSKAVAESYVEKYAAASGSSYYIFRYPNVFGKWCKPNYNSFIATFCYNISNDIEIT
                     INDAAAPVNLVYIDDVCTDAIALLSGTVESGYKVVAPIYSTTVGEVAELIYSFKNSRS
                     TLITEAVGAGFTRALYSTWLSYLPAEKFAYKVPFYGDARGVFCEMLKTPSAGQFSFFT
                     AHPGITRGGHYHHSKNEKFLVIRGQACFKFEHVITGERYELKVSSGEFKIVETVPGWT
                     HDITNIGTDELIVMLWANEIFNRDEPDTIARPL"
     gene            2209..3339
                     /gene="wecB"
                     /locus_tag="ACG0BW_002739"
     CDS             2209..3339
                     /gene="wecB"
                     /locus_tag="ACG0BW_002739"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFK7259682.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKFDEYCEHIIVHTGQNYDYE
                     LNEVFFNDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSCI
                     SAIPAKRRKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEGI
                     PADRIIKTGSPMFEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLVK
                     LANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNSR
                     AVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            3339..4550
                     /locus_tag="ACG0BW_002740"
     CDS             3339..4550
                     /locus_tag="ACG0BW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFK7259683.1"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRAVNETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGPLVKKIKARCQCPAYLILRDMFPQWVIDAGMLNAGSPIERYFRLFEKI
                     SYRQANRIGLMSDKNLDVFRKDNTGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAAYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGSGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            4537..4935
                     /locus_tag="ACG0BW_002741"
     CDS             4537..4935
                     /locus_tag="ACG0BW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFK7259684.1"
                     /translation="MRLIDNDQLDELYDQAGQSERLRSHLMMHGSHQEKVQRLLIALV
                     KGSYVEPHYHELPHQWEMFIVMEGQLQVCLYGRNGEVIKQFIAGDNTGMSIVEFSPGD
                     IHSVECLSPRALMVEVKEGPFDPSFAKSFV"
     gene            5053..6459
                     /gene="gndA"
                     /locus_tag="ACG0BW_002742"
     CDS             5053..6459
                     /gene="gndA"
                     /locus_tag="ACG0BW_002742"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFK7259685.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            6708..7874
                     /gene="ugd"
                     /locus_tag="ACG0BW_002743"
     CDS             6708..7874
                     /gene="ugd"
                     /locus_tag="ACG0BW_002743"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFK7259686.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPMLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            8020..9000
                     /gene="wzzB"
                     /locus_tag="ACG0BW_002744"
     CDS             8020..9000
                     /gene="wzzB"
                     /locus_tag="ACG0BW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFK7259687.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEREKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9097..9708)
                     /gene="hisIE"
                     /locus_tag="ACG0BW_002745"
     CDS             complement(9097..9708)
                     /gene="hisIE"
                     /locus_tag="ACG0BW_002745"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFK7259688.1"
                     /translation="MLTEQLRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(9702..10478)
                     /gene="hisF"
                     /locus_tag="ACG0BW_002746"
     CDS             complement(9702..10478)
                     /gene="hisF"
                     /locus_tag="ACG0BW_002746"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFK7259689.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINISELKTYLATQGVEIRIC"
     gene            complement(10460..11197)
                     /gene="hisA"
                     /locus_tag="ACG0BW_002747"
     CDS             complement(10460..11197)
                     /gene="hisA"
                     /locus_tag="ACG0BW_002747"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFK7259690.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(11197..11787)
                     /gene="hisH"
                     /locus_tag="ACG0BW_002748"
     CDS             complement(11197..11787)
                     /gene="hisH"
                     /locus_tag="ACG0BW_002748"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFK7259691.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(11787..12854)
                     /gene="hisB"
                     /locus_tag="ACG0BW_002749"
     CDS             complement(11787..12854)
                     /gene="hisB"
                     /locus_tag="ACG0BW_002749"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFK7259692.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVIITNQDGLGTQSFPQADFEGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVARNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(12854..13924)
                     /gene="hisC"
                     /locus_tag="ACG0BW_002750"
     CDS             complement(12854..13924)
                     /gene="hisC"
                     /locus_tag="ACG0BW_002750"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFK7259693.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALQEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(13921..15225)
                     /gene="hisD"
                     /locus_tag="ACG0BW_002751"
     CDS             complement(13921..15225)
                     /gene="hisD"
                     /locus_tag="ACG0BW_002751"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFK7259694.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            complement(15231..16130)
                     /gene="hisG"
                     /locus_tag="ACG0BW_002752"
     CDS             complement(15231..16130)
                     /gene="hisG"
                     /locus_tag="ACG0BW_002752"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFK7259695.1"
                     /translation="MTDNSRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(16178..16301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(16277..16327)
                     /gene="hisL"
                     /locus_tag="ACG0BW_002753"
     CDS             complement(16277..16327)
                     /gene="hisL"
                     /locus_tag="ACG0BW_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFK7259696.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            16754..17578
                     /gene="yeeZ"
                     /locus_tag="ACG0BW_002754"
     CDS             16754..17578
                     /gene="yeeZ"
                     /locus_tag="ACG0BW_002754"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFK7259697.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            17624..18553
                     /gene="yeeY"
                     /locus_tag="ACG0BW_002755"
     CDS             17624..18553
                     /gene="yeeY"
                     /locus_tag="ACG0BW_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFK7259698.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASAWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            18768..18830
                     /gene="yoeI"
                     /locus_tag="ACG0BW_002756"
     CDS             18768..18830
                     /gene="yoeI"
                     /locus_tag="ACG0BW_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFK7259699.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            18820..20178
                     /gene="plaP"
                     /locus_tag="ACG0BW_002757"
     CDS             18820..20178
                     /gene="plaP"
                     /locus_tag="ACG0BW_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFK7259700.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT         5064 a         4720 c         5053 g         5410 t
ORIGIN      
        1 taataagtta ggtcaagtaa gtaaatagct tccagagtat atttgtcaat gatttgaggt
       61 tcggttatta tgttttcatc taaaacactg ttaattactg gtggtactgg ctctttcggg
      121 aatgctgtat taaatagatt tcttgataca gatattgcag aaatccgtat atttagtcgt
      181 gatgaaaaaa aacaagatga tatgcggaaa aaatacaata atcaaaaatt aaagttctat
      241 attggtgatg tcagagatta ccgtagtatt ttgaatgcga ctcgcggtgt tgattttata
      301 tatcatgcag cggcacttaa gcaagttcca tcatgtgaat ttcatcctat ggaagccgtt
      361 aaaactaata tccttggtac ggaaaatgtt cttgaagcag ctatagcgaa tgaagtgaag
      421 agggttgtat gcctaagtac tgataaagct gtatacccga ttaacgcaat gggtatttca
      481 aaagctatga tggaaaaggt catggtcgcg aaatcccgta atgttgatcg caataaaaca
      541 gtaatatgtg gtacccgtta tgggaatgtt atggcatctc gcggttcagt tattccatta
      601 tttgttgatc ttattagagc gggcaagcca ctcacaataa ctgatcctaa tatgacccgc
      661 tttatgatga ctcttgagga tgcggtagat ttagttcttt atgcgtttga acatggtaat
      721 aatggtgata tctttgtgca aaaagcacct gcagcaacta ttgacacatt agctattgct
      781 ttaaaggaat tactaaatgt tcctgaccat ccggtaaatg tcattggaac gcgtcatggc
      841 gagaaattat atgaagctct acttagtcgt gaggaaatga tcgctgctat agatatgggc
      901 gattattacc gtgtcccgcc agatcttcgt gaccttaatt atggcaaata tgttgagcaa
      961 ggtgatagcc gaatatctga aatagaagat tataactctc ataatactca acggttagat
     1021 gttgaaggca tgaaagagct cttgctaaaa ttagccttta ttcgagcaat tcgtgctggt
     1081 gaaaaatata atctggattc atgatatgaa aatattagtt actggtgcaa atggttttat
     1141 tggtcgtaat ttatgtttga ggcttgagga acttggttat aaagatctta ttagaattga
     1201 tcgagaatca acgaagcaag atcttgaaca aggcttacag gatgccgatt ttatttatca
     1261 cttagctggt atcaatagac ctaagactga tgatgagttt atttctggaa acagtgattt
     1321 aacaaagcat atagttgagt atctcctttc tattggtaag aatacaccaa ttatgctaag
     1381 ttcttcgata caagctgaac ttaataatgc ttatggggtt agcaaagctg tagctgaaag
     1441 ctatgtcgaa aaatatgctg ctgctagtgg ttcttcgtat tatattttca gatatccaaa
     1501 cgtttttggt aaatggtgta agccaaacta taattctttt atagcaactt tttgctacaa
     1561 tatttccaat gatattgaga ttactatcaa tgatgcagca gcgccagtca atctggtcta
     1621 tattgatgat gtttgtactg atgctatagc tcttctctct gggacggttg aaagtggata
     1681 taaagttgtt gcaccaattt attcaacaac agttggtgaa gttgcagaat taatttatag
     1741 cttcaaaaat agccgttcca ccctgatcac agaggctgtc ggggcgggat ttacccgtgc
     1801 attgtattct acatggctga gttatttacc agcagagaag tttgcgtaca aggtaccttt
     1861 ttatggggat gcccgcggag tcttttgtga gatgttgaaa acgccttcag cggggcagtt
     1921 ttcatttttt actgctcacc ctggtattac gcgtggcgga cattaccatc acagtaaaaa
     1981 tgagaagttt ttggtcattc gaggtcaggc atgctttaaa tttgaacatg tgattaccgg
     2041 tgagcgatat gaactgaaag tttcatcggg tgagtttaag attgttgaaa cagttcctgg
     2101 ttggacacat gacattacaa atattggaac tgatgaatta atagtcatgc tctgggcaaa
     2161 tgaaattttc aaccgtgatg agcccgatac tattgcgaga cctctataat gaaaaaatta
     2221 aaagttatgt ctgttgttgg aacccgtcct gagattatcc gtttgtcgag ggttcttgct
     2281 aagtttgatg aatactgcga gcatattatt gtccatactg gtcaaaatta tgattacgaa
     2341 ttaaatgaag tgttcttcaa tgacttgggt gttcgaaaac ctgattattt tttaaatgca
     2401 gcgggtaaaa atgcggcgga aaccattggt caggttatta ttaaggtaga tgaagtatta
     2461 gaaatcgaaa aacctgaagc aatactggta ttgggcgata cgaattcatg tatttctgcc
     2521 attccggcca aacgccgtaa agtgcctata tttcatatgg aagcaggtaa ccgttgtttc
     2581 gatcaacgcg tgcctgaaga aaccaacaga cgtattgttg accatacggc tgatatcaat
     2641 atgacctaca gtgatattgc tcgtgaatat ctcttggctg aaggtatccc agctgatcgg
     2701 atcataaaaa ctggtagccc tatgtttgag gttctttcat attatatgcc ccaaattgat
     2761 ggttcagatg tgctatcgcg tttgaatcta cagtctggtg agttttttgt agtaagtgcg
     2821 catcgtgaag agaatgttga ttctccaaaa cagctcgtaa agcttgcgaa cattctaaat
     2881 actgttgctg aaaaatataa tcttccagtt attgtctcca cacacccaag gacacgtaac
     2941 cgaatccgtg agcaaggaat tgaatttcat tcaaatataa atctactgaa accattgggt
     3001 ttccatgatt ataaccactt gcagaagaac tcacgagctg tgctttcaga tagcggtact
     3061 atcactgaag agtcatccat catgaatttc ccagcggtaa acatccggga agcgcatgag
     3121 cgtccggaag gctttgagga agcatccgtc atgatggtgg ggttagagtg tgaacgcgta
     3181 ttacaagcgc tggatattct ggcaacacaa ccgcgaggtg aagtccgtct tttacgtcag
     3241 gttagtgatt acagcatgcc aaatgtgtcg gataaagttg tcagaattgt tcactcttac
     3301 acagattatg ttaagagagt cgtctggaaa gaatattgat gaaacttgct ttaatcatag
     3361 atgattacct gcccaacagt actcgtgttg gtgcaaaaat gtttcatgaa cttgctcaag
     3421 aatttatcca gcgtgggcac gatgttacgg taattactcc tggtacgggc atgcaagaag
     3481 agatttcttt tgataccttt cagggggtaa aaacatggcg ttttaaaagc gggccgctca
     3541 aggatgtaag taaaattcag cgagcggtca atgaaacgct tttgtcctat cgggcgtgga
     3601 aagccatcaa aaaatgggta aaaaaagaga cctttgaggg ggtgatttat tattcacctt
     3661 ccatattctg ggggccttta gttaaaaaaa ttaaagctcg ttgccaatgt cctgcttatc
     3721 ttattttaag agatatgttt ccacaatggg taattgatgc aggaatgctt aatgctggtt
     3781 ccccaataga acgctacttt cgtctttttg aaaaaatatc ttatcgtcag gcaaatcgta
     3841 ttggacttat gtctgataag aatcttgatg tttttcggaa agataataca ggctatccgt
     3901 gcgaagtttt gcgtaattgg gcatccctaa caccaacgat catacccaag gattatatac
     3961 cactacgtaa gcgacttggc ctagaggata aaaccatttt cttctatggt ggaaacatag
     4021 gtcatgcaca ggacatgaca aacttgatgc gacttgtgag aaacatggca gcatatcctc
     4081 aagctcattt cctatttatt ggccaggggg atgaagttga attaattaat tcattagcat
     4141 ctgagtgggc attgacgaat ttcacctatt tgccctcggt taaccaagat gaatttaagt
     4201 tcattttgtc ggaaatggat atcggcttgt tttctctttc cgctagacac tcttcccata
     4261 attttcctgg taagttatta ggctatatgg ttcagtcgct acctatttta ggtagcgtaa
     4321 atgccggaaa tgatttgctc gacattgtca atcaaaataa tgcgggatta atccatgtca
     4381 atggtgagga cgataaatta tgtcaatctg cgctattaat gttgcatgat attgatgtgc
     4441 gccggcaact tggttcgggg gcgaatatat tgttgaaaga acaattctcc gttgagtctg
     4501 cggcacagac gatagaaatg aggttggagg catgcaatgc gattaattga taatgaccaa
     4561 ctcgacgaat tatatgatca agccgggcaa tcggaacgtt tacgttccca ccttatgatg
     4621 cacggctcgc atcaagaaaa ggtacagcgt ttacttattg cattagtaaa gggcagctat
     4681 gttgaaccgc attatcacga acttcctcat cagtgggaaa tgttcattgt tatggagggg
     4741 caacttcagg tttgtttgta tggtagaaat ggtgaggtta taaagcaatt tatagcagga
     4801 gataatactg gaatgagcat tgtggagttt tctccgggcg atatacacag tgtcgaatgc
     4861 ctatctccgc gtgctcttat ggtggaagtt aaggaggggc catttgaccc ttcttttgca
     4921 aaatcgttcg tgtgatgctt gtctaaagta catcttctgc tatctaccca agctaaacct
     4981 gagttaacat ccataccata tttcaagctg cgcatatctt gcgcggtgac caccccctga
     5041 caggagtaaa caatgtcaaa gcaacagatc ggcgtagtcg gtatggcagt gatggggcgc
     5101 aaccttgcgc tcaacatcga aagccgtggt tataccgtct ctattttcaa ccgttcccgt
     5161 gaaaagacgg aagaagtgat tgccgaaaat ccaggcaaga aactggttcc ttactatacg
     5221 gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca tcctgttaat ggtgaaagca
     5281 ggtgcaggca cggatgctgc tattgattcc ctcaaaccat atctcgataa aggtgacatc
     5341 atcattgatg gtggcaatac cttcttccag gacaccattc gtcgtaaccg tgaactttct
     5401 gccgaaggct ttaacttcat tggtaccggt gtctccggtg gtgaagaagg cgcgctgaaa
     5461 ggtccttcta ttatgcctgg tgggcagaaa gaagcctatg aactggttgc accaatcctg
     5521 accaaaatcg ccgcagtagc tgaagacggg gagccatgcg ttacctatat tggtgccgat
     5581 ggcgcaggcc attatgtgaa gatggttcac aacggtatcg aatacggtga tatgcaactg
     5641 attgctgaag cctattctct gcttaaaggt ggcctgaacc tcaccaacga agaactggcg
     5701 cagaccttta ccgagtggaa taacggtgaa ctgagcagtt acctgatcga catcaccaaa
     5761 gatatcttca ccaaaaaaga tgaagacggt aactacctgg ttgatgtgat cctggatgaa
     5821 gcggctaaca aaggtaccgg taaatggacc agccagagcg cgctggatct cggcgaaccg
     5881 ctgtcgctga ttaccgagtc tgtgtttgca cgttatatct cttctctgaa agatcagcgt
     5941 gttgccgcat ctaaagttct ctctggtccg caagcacagc cagcaggcga caaggctgag
     6001 ttcatcgaaa aagttcgtcg tgcgctgtat ctgggcaaaa tcgtttctta cgcccagggc
     6061 ttctctcagc tgcgtgctgc gtctgaagag tacaactggg atctgaacta cggcgaaatc
     6121 gcgaagattt tccgtgctgg ctgcatcatc cgtgcgcagt tcctgcagaa aatcaccgat
     6181 gcttatgccg aaaatccgca gatcgctaac ctgctgctgg ctccgtactt caagcaaatt
     6241 gccgatgact accagcaggc gctgcgtgat gtcgttgctt atgcagtaca gaacggtatt
     6301 ccggttccga ccttctccgc agcggttgcc tattacgaca gctaccgtgc tgctgttctg
     6361 cctgcgaacc tgatccaggc acagcgtgac tattttggtg cgcatactta taagcgtatt
     6421 gataaagaag gtgtgttcca taccgaatgg ctggattaat ctgatttaac caacaataaa
     6481 attgaggccc ggcgtatatt gcaccgggct tttttttgcc aaatatctta tagactaaat
     6541 ttcactgctt aatattaact taataaatat cagctattct tataaagaaa atctgaattg
     6601 tttttcgttg cgttgaccat cgaacaacgt agcgttaaaa cttttagctc ttatcaggat
     6661 gctaaaaaca tcatgattca cagttaagtt aattctgaga gcatgaaatg aaaatcacca
     6721 tttccggtac tggctatgta ggcttgtcaa acgggcttct aatcgcacaa aatcatgagg
     6781 ttgtggcatt agatatttta ccgtcacgcg ttgctatgct gaatgatcgg atatctccta
     6841 ttgttgataa ggaaattcag cagtttttgc aatcagataa aatacacttt aatgccacat
     6901 tagataaaaa tgaagcctac cgggatgctg attatgttat catcgccact ccaaccgact
     6961 atgatcctaa aactaattat ttcaatacat ccagtgtaga atcagtaatt aaagacgtag
     7021 ttgagataaa tccttatgcg gttatggtca tcaaatcaac ggttcccgtt ggttttaccg
     7081 cagcgatgca taagaaatat cgcactgaaa atattatatt ctccccggaa tttctccgtg
     7141 agggtaaagc cctttacgat aatctccatc cttcacgtat tgtcatcggt gagcgttcag
     7201 aacgcgcaga acgtttcgct gctctgttac aggaaggcgc gattaagcaa aatatcccga
     7261 tgctgtttac cgactccact gaagcagaag cgattaaact ttttgcaaac acctacctgg
     7321 cgatgcgcgt ggcgtacttt aacgaactgg atagctatgc agaaagttta ggtctgaatt
     7381 cccgtcaaat aatcgaaggc gtttgtctcg acccacgtat tggcaaccat tacaacaatc
     7441 cgtcgtttgg ttatgggggt tattgtctgc cgaaagatac caagcagtta ctggcgaact
     7501 accagtctgt gccgaataac ctgatctcgg caattgtcga tgctaaccgc acgcgtaaag
     7561 attttattgc cgatgccatt ttgtcacgca agccgcaagt ggtgggtatt tatcgtctga
     7621 ttatgaagag cggttcagat aacttccgtg cgtcttctat tcaggggatt atgaaacgta
     7681 tcaaggcgaa aggtgttgaa gtgatcatct acgagccagt gatgaaagaa gactcattct
     7741 tcaactctcg cctggaacgt gatctcgcca ccttcaaaca acaagccgac gtcattatct
     7801 ctaaccgaat ggcagaagag cttaaggatg tggcagataa ggtatacacc cgcgatctct
     7861 ttggcagcga ctaacatctt gttatcaggg ctatttacgc cctgattgtc ttttgttact
     7921 tacacaacaa ttcattattt ttatcactta tcctatagca ttcacgagga ttatcgctaa
     7981 actatgcgga cttggaaatt tccgtcagtt agggtaatga tgagagtaga aaataataat
     8041 gtttctgggc aaaaccatga cccggaacag attgatttga ttgatttact agtgcagttg
     8101 tggcgtggca agatgacaat catcatttcc gtcattgtgg ctattgccct ggctattgga
     8161 tatttggcag tagcgaagga gaaatggacg tcaacagcaa ttatcactca gcccgacgtg
     8221 gggcaaattg ctggctataa caatgccatg aatgttatct atggtcaggc tgcaccgaaa
     8281 gtatcggatt tgcaggagac gttaattggt cgcttcagtt ctgccttctc tgcattagca
     8341 gaaacgctgg ataatcagga agaacgagaa aaacttacca tcgaaccttc tgttaagaac
     8401 cagcaattac cattgactgt ttcttatgtt gggcaaactg cagagggcgc acaaatgaag
     8461 ttggcccaat acattcagca agttgacgat aaagtgaatc aagagctaga aaaggatctc
     8521 aaagacaaca ttgctctggg acggaaaaac ttgcaggact ctttaagaac gcaggaagta
     8581 gtcgcgcagg agcagaaaga tctgcgtatc cgtcagattc aggaagcgtt gcagtatgcg
     8641 aatcaggcgc aggtgacaaa accgcagatt caacagactg gcgaagatat cacacaagat
     8701 acgttgttcc ttttggggag cgaagcgctg gagtcgatga ttaagcacga ggcgacccgt
     8761 ccgttggtgt tctcgccaaa ctactatcag actcgtcaaa acctgctgga tatcgaaagc
     8821 ttaaaggttg atgatcttga tattcatgct taccgttatg tgatgaaacc gacgttacct
     8881 attcgtcgcg atagtccgaa aaaggcaatt accttgattc tggcagtgct gctgggcggc
     8941 atggttggcg cggggattgt gttggggcgt aacgctctgc gtaattacaa cgcgaagtaa
     9001 tctttgcagt tttaaagaaa aagggcaggg tggtgacacc ttgcccgttt tttttgccgg
     9061 atgcgacaac aatatcgcat ccgcttaccc cgcaactcac tgatgtcgtt tacgcaggtt
     9121 ctcaattacc gtcgttaaat ccagatcctg atcctgtaac agcaccagca ggtgatacat
     9181 caaatcagag gcttcgttgg ttaattcgaa gcggtcattg accgtcgcgg ccagcgcggt
     9241 ttccacgcct tcttcaccca ctttctgcgc aatgcgtttg gtgccgctgg catacagttt
     9301 ggcggtgtag gaggtttccg gatcggcaga tttgcgctcg gcgagcagtt gttccagttg
     9361 atacaggaac agccactggt gagcggtgtc gccgaagcaa ctgctggtgc ctttgtggca
     9421 ggtcgggccg atgggattcg ccagcaccag taacgtgtcg ttgtcgcagt ccggcgcaat
     9481 atttactacg ttgaggaaat tgcccgacgt ttcgcctttg gtccacagtc gctgtttagt
     9541 gcgcgagaag aaggtgactt tgccgctttc gatggttttg tccagagctt ccgggttcat
     9601 atagcccagc attaacactt cgccagatac cgcgtgttgc acaatcaccg gcataagtcc
     9661 gtcggttttt tcccagtcca gttcgcgacg tagttgttct gttaacatat cctgatctcc
     9721 acgccctgtg ttgccaggta cgtttttaat tcactaatat tgataatttg tttgtggaat
     9781 acggaagctg ccagcgcacc gtcaacgtcg gcatcgcgga aggcttcgag gaagtgttcc
     9841 atggtgcccg cgccaccgga ggcaatcagc gggacgtggc aaacttcacg cacttttttc
     9901 agctgttcga ggtcgtaacc gttacgcacg ccgtcctgat tcatcatatt gaggacgatt
     9961 tctccggcac cgcgtttttg cacttcctgt acccagtcga gcgtttccca ctgagtgacg
    10021 cgggtgcggc tttcatcgcc ggtatattga ttcacatgat atttaccggt ttcggcgtcg
    10081 taccaggtat caataccaac cacaatacac tgcacgccaa agcgatccgc caggcgagta
    10141 attaacgtcg ggtccgcaag cgcaggggag ttaatggaga ttttatccgc gccaaaggaa
    10201 agaattttgg cggcgtcatc cagcgattta atcccacccg ccacgcagaa cggaatgtcg
    10261 atgacttccg ccacgcgaga tacccagctt ttatctacca cacgaccatc gctggaagcg
    10321 gtgatatcgt agaacaccag ttcgtcagcg ccttcttcgg cgtagcgttt tgccagcggt
    10381 acaatatcgc caatgatttc atggttgcga aactgtacgc ctttcaccac ctgaccatca
    10441 cgaacgtcga gacatggaat tatgcgtttt gccagcatgc gatggcctcc ttcacggtga
    10501 atttaccttc cagtaatgcc cgaccaacta ttacgccgcg cacgccagtg ccacgcaggg
    10561 cagcaacatc gttaatgtcg ccaatgccgc cggatgactg aaatgccacc tgcggatatc
    10621 tggcgcacac ttcttcatat aaagagacgt tagagcctgc cagcgtgccg tcgcgcgaga
    10681 tatcggtaca cagcacatgt ttcaggccga cgggcagata ggtttccacc agttgttcca
    10741 gcgacacacc cgagttctct tgccagccgc tgactgccac ctgcttgttg ccttgctcgt
    10801 caatacggac atccagcgcc agcactaagg catcggcacc gaaacgttca aaccagcctt
    10861 tcaccatttc tggtgatttc accgcggtgg agccaaccac cacgcgcgca acgcctgctt
    10921 ccagtaacgc cgccacgtct tcttcgctac gcacgccgcc gccaacctgc accgggacgt
    10981 taacgcccgc gaccagggtt ttaatcagcg ggatttgacg cttagccgga tcttttgccc
    11041 cggtcagatc caccaggtgc aacacttcgg caccttgcgc agcgtaatcc tgtaagcgtg
    11101 gcagcgggtc gttaccgtaa tcgcgttgtt tgccgtaatc gccctgatgg agacgcacca
    11161 cggtgccgtc gattaaatct aatgccggaa taatcatcac atctccagga aatttttcag
    11221 caacttagcg ccagcggcac cagaacgctc cgggtggaac tgcacgccgt agaagttatc
    11281 tttttgtacc gccgcggtga acggttcgcc gtaattacac tgggcgatgg tccacggatt
    11341 gactggcatt gcgtagctgt gaacaaagta aaagtacgcg ccgtcttcaa tcccctgaaa
    11401 caggcggttg cctgcctgcg gataaacgcg gttccagccc atatgtggca gtggcagacc
    11461 aaagtcggtc attttcggca cgtcttcgtc gatgatgccc agcaagtcga cgccgttgct
    11521 ctcttcgctg cgccgtccca gcagttgcat ccctaagcag atgcccagca ccggttgggt
    11581 acaggctttg atgagatcaa acagctcgcg ctcacgcacc tggtccatcg ccgcttgcgc
    11641 ggtgccaacg ccgggtaaaa acagtttatc ggccagtaac acgacgtcag ggtcacggct
    11701 gactttgggt tcataaccgt ggcgcgcaat ggcagacttc accgagttca ggttggcgca
    11761 gccagtatca aggatcacca cgttcattac agcactcctt tcgacgaggg cagggtgtcg
    11821 ccttccacgc ggatggcctg gcgcagggtg cgaccaaagg ctttaaagag actctctaca
    11881 cggtggtgat cgtttttacc tttggttttc aggtgtagcg tcacgcccat ggtgtatgag
    11941 agcgaacgga agaagtgctc gatcatctcg gtgctgagat cgcccacgcg ctggtaggta
    12001 aattcagctt tatattccag gtgcgggcga ccagagatat ccagcgcgca gcgggcaagg
    12061 cattcgtcca tcggcagcac aaaaccgaag cggcaaatac cgcgtttgtc gccaagagca
    12121 atttttaatg cttcccccag cgccaggccg gtatcttcga cggtgtgatg atcgtcgata
    12181 tagaggtcgc ctttaacgtt gatttccata cggaaaccac cgtgggtagc gatctgatcc
    12241 agcatgtgat caaagaagcc aacgccggtg ttaatcttgc tgccaccttc gcgatccagc
    12301 cacacctgga cgtcaatttg cgtctctttg gtgttacgcg ctacatgggc gtatcggtct
    12361 cgtttagtga gttgctcgcc aatcattggc cagttcaggg tttcgcggtc gtagcgtaaa
    12421 ccattaatgc ccatgttttc cgccagttga atgtcggtcg cgcgatcgcc aatcacataa
    12481 ctgttggcgc gatccatcgc ttgctcagcc agataacgct ccaccagttt tactttcggc
    12541 ttacggcagt cgcactcatc ggcgggcagg tgcggacaaa tcagcacttc atcaaactgc
    12601 acgccttgcg aggtgaagat ctgcatcatc aggttgtgcg ggccttcgaa atccgcctgc
    12661 gggaaacttt gtgttcccag accatcctga ttagtgatta tcaccagctt gtagcccgct
    12721 ttttgcagct tcagcagctc cgggatcacg cccggctcaa aggcgagttt gtcaaaacga
    12781 tccacctgaa aatcactcgg cggttcgcta atcagggttc catcgcgatc gataaaaaga
    12841 tacttctgac tcatcaaact tgctccgcac gtaaggcgtc aatgacgcgc tggctttctt
    12901 cacgggttcc gacggtaatt cgcaggcagc cgcttaaaga gggttgttta ttctgatcac
    12961 gtaagataat gccctgatcc cacaaagatt taaacactgc gctggaggct ttaaagcgcg
    13021 ccagaatgta gttggtttca gagtcaaaaa cctgctccac gcaggggatc tcttgcagtg
    13081 cggcaatcag gtattcgcgt tcagcaataa tttgcgccac tcgttcgcgc atggcgacga
    13141 ttccctgcgg gcttaacgcc tgtgcggcaa tgtcggcaac cggcgtcgag agcgggtagg
    13201 gggcgatcac tttcatcagc aggttgatga cttcttcgtt tgccagcgta aatccgcaac
    13261 gaagccccgc cagagcaaaa gctttcgaca gtgtgcgcaa aatcgccagg tgcggatact
    13321 ccgccagcca gccagccagc gatgcctgcg ggcaaaactc gatataggct tcatcggcaa
    13381 ccacaatcgc cttaccgcgg gttaactcca gcagggtgcg aaaatcctgc gggttgatca
    13441 gttgcccggt ggggttgttg gggctgcaaa catagaccac ttttacgccg tccagcttgt
    13501 cggaaatgcc ctgtaagtcc agttgccagt tgtccagcgt cggcactgtg cggcactcga
    13561 cgccaatggt ttcagcgctg acgctgtaca tgccgtacgt tggcgggcag tagaggatgg
    13621 cgtctttacc cggttcgcaa aaagcgcgaa tcagcagctc aataccttcg tccgcgccac
    13681 ggctgaccag cacctgttcc ggttttacgc ctgcatattg cgcgtaattt tcaatcaccg
    13741 ctttcggttg gcattccgga tagcggttga gcgtttgctg agtgagctga aactccacgg
    13801 cagtgggata ttcgttggcg ttcagccaga catcgccgtt accgcccaga cgacgcgctg
    13861 actgatacgg cgtcaggttg cggacgtttt cacgcgctaa atcggtaata gtcacggtgc
    13921 tcatgcttgc tccttaaggg cgttaacacg caaagtaacg gcatttttgt gggcggtcag
    13981 gcgctcggcg gcggccagtg tttcaatggt cgaagccagc gcggagaaac cttctttcga
    14041 gagttcctgc acggtcatgc gcttctggaa atccgccagc ccgaggctgg aacaggtggc
    14101 ggtgtaaccg taagtcggca gaacgtggtt agtgccggag gcatagtcgc ctgccgattc
    14161 cggtgaccag tcaccaagaa ataccgaacc ggcgctggtg atgccatcga ccagttcgcg
    14221 ggcgttgcgg gtctgaatga tcaggtgctc cgggccgtac tggttggaga tctctacgca
    14281 ctgcgctaaa tctttagtca cgatcaggcg gctggcgttc agtgcctggc gggcggtttc
    14341 ggcacgcggc agttctgcca gttggcgttc gacggcctcg gcaacgcgac gcgccatatc
    14401 agcgtcgggg gtcagtaaaa ttacctgtga gtcagggccg tgttcagcct gagaaagcaa
    14461 atcagaagcc acgaaatccg gcgttgcgcc gctgtcagca atcaccagca cttccgacgg
    14521 gcctgcgggc atatcgatcg ccgcaccgtc cagacgctgg cttacctggc gttttgcttc
    14581 ggtgacaaag gcgttacctg gcccgaagat tttgtccact ttcggcacgg attctgtacc
    14641 aaacgccagc gcggcaatgg cctgtgcgcc gccgacgtta aacacatcct gcacaccgca
    14701 cagctgtgcc gcgtaaagga tctcatcggc aatcggcggc ggtgagcaca gtacgacttt
    14761 tttacagccc gcaatacgcg ccggagtcgc cagcattaat accgttgaga agagcggggc
    14821 ggagccgcca ggaatataca acccaactga agctaccgga cgcgtgactt gctggcaacg
    14881 tacgcctggc tgcgtttcta catctaccgg cggcagtttt tgcgcagtgt ggaaggtttc
    14941 aatattcttt actgccaccg ccatcgcctg ttttagctcg tcgctcaggc gttcgctggc
    15001 ggcggcgatc tcatcagcag acaccttcag cgcggtaacc gtggttttat caaacttcgc
    15061 gctgtattcc cgcaaggcct catcgccacg cgttttcacg ttatcgagaa tatcgttaac
    15121 agtgcgggta atgctttcag aggcggagat cgccgggcgc attaacagct ggcgttgttg
    15181 ctccgcagta cagctattcc agtcaatgat tgtgttaaag ctcatggcga tcactccatc
    15241 atcttctcaa tcggcaggac caggattgaa ctggcaccca gcgctttcag tttttccatc
    15301 gtttcccaga acagggtttc gctgctgacc atgtgcatcg ctacgcgctg ttggtcacct
    15361 gccagcggca gaatagttgg gcgttcggca cctggcagca gggcgatgac ttcatccaga
    15421 cgttcggtcg gtgcgtgcat catgatgtat tttgattcgc gcgcctggat cacgccctga
    15481 atacgggtca gcagcttgtc gatcagttgc tgtttagatt cttccatttc gccatcgcgc
    15541 tggatcaggc aagctttcga gcggtagata acttcgactt cgcgcaggcc gttagcttcc
    15601 agcgtggcac cggtggaaac cagatcgcaa atcgcatccg ccagtccggc acgcggggcg
    15661 acttcaacag aaccgttcag taagcaggat ttaaaagaga tacctttctg gtcgagataa
    15721 cgcttgagca ggtgaggata agaggtggcg atacgtttac cgtttaagga gagcggaccg
    15781 tcccaggctt catcaaccgg cgttgccagc gaaaggcggc agccgccgaa atccagacga
    15841 cgcagggtaa agtagcgcgg atcttcaccc tgggcgcggc ggttaagcag ctcttcttcc
    15901 agcacgtttt cgccgataat cccaaggtct accacgccgt ccattaccag accgggaatg
    15961 tcgtcgtcac gtacgcgcag aatatcaatc ggcatgtttt ctgccatcgc gatcaggcgc
    16021 tgggtgtgaa gattgatttt aatgccacag cgcgccagca attcgcgtga gtcatcactt
    16081 aaacggccgg atttctgcat agctatgcgt aaacgagagt tgtctgtcat tttcttattc
    16141 ctctttaaaa cctgtctgaa ccgttatcaa ccgcgcaata aaaaagcccc cggaaggtga
    16201 tcttccgggg gctttctcat gcgttcatgc accactggaa gatctgaatg tcttccagca
    16261 cacatcgcct gaaagactag tcaggatgat ggtgatgatg gtggtgttta aattgaacgc
    16321 gtgtcataaa agtctctgtg aatgtttatt caactgatgt cttttaacct aaaccacttt
    16381 cacgttggaa agcaaggggt tttttatata ttttatggcg atggatttgt ttaatgaatg
    16441 agcggggaaa ggagggggcc aggagtggct acgttaaaaa agtaatcgtg aaaatatgtc
    16501 tttaaaaccc tcaagaacat tgtgttctgt aactaaagtg ggctttgttc ctgccggatg
    16561 cggcgtgaat gccttatctg gtctacaaat gttgcctaaa ttcaatatat tgatgttttt
    16621 gtaggactga taagcggagc acatcagaca attgtatatt tgctaacagt ttcgatttca
    16681 ttcatagaat gaattgtcag ttttgatacg ctagcgtagc cttatagatc taaggtgtat
    16741 caggagataa cggatgaaaa aggtcgcaat tgtcgggtta gggtggttag gcatgccgct
    16801 ggcgatgtca ctttcagcgc gaggctggca agttaccggg agtaaaacca cacaagatgg
    16861 tgtcgaagcg gcccgaatga gtggcattga tagctatctg cttcgcatgg agcctgagtt
    16921 agtttgcgat tctgacgatc tggatgccct gatggatgcc gatgcgctgg tcattacgct
    16981 tccggcacgt cgtagcggcc ccggcgatga gttctattta caagcggtac aagagttagt
    17041 agatagcgcg ctggcccatc gtattccccg cattattttt accagctcaa catctgtcta
    17101 tggcgacgcg caaggcacgg tgaaagaaac caccccgcgt aatccggtaa ccaacagtgg
    17161 acgagtatta gaagaactcg aagactggct gcacaattta cccggaactt cggtcgatat
    17221 tctacgtctt gcgggcctgg tcggaccggg acgtcatccc ggacgtttct ttgccggaaa
    17281 aaccgcccct gatggtgaac atggtgttaa tttagtccat ttagaagatg ttattggcgc
    17341 tatcactctg ttgttacagg cacctaaagg cggacacatc tataatatat gtgcgccagc
    17401 tcaccctgcg cgtaatgttt tctatccgca gatggcccgt ttactgggac tggaacctcc
    17461 gcagttcaga aatagcctgg acagcggaaa aggtaagatt attgatggca gtcggatttg
    17521 taatgaactg ggatttgaat accagtatcc cgatccgctg gtaatgccgc tggagtaaac
    17581 gatcacgcca gcaggacgcc gcatgcactg caagggggcg tgtatgaaac cactgctgga
    17641 tgtgttgatg atcctcgatg ccttagaaaa agaaggcagt tttgcggcgg cgtcggccaa
    17701 actctataag acgccgtccg ctctgagtta caccgttcac aagctggaaa gcgaccttaa
    17761 cattcaattg cttgatcgta gcggtcaccg cgctaaattc acccgcaccg gaaaaatgtt
    17821 attagagaag gggcgggaag ttctgcatac cgtgcgagaa ctggaaaagc aggcgataaa
    17881 actgcatgaa ggctgggaaa acgagctggt gattggcgtg gacgacacgt ttcctttttc
    17941 tctgcttgcg ccactcattg aagcttttta tcaacatcat agcgtgacgc gcctgaagtt
    18001 tatcaatggc gtactcggtg gttcatggga tgccctgact caggggcgag cggatattat
    18061 tgttggcgcg atgcatgagc caccatcttc cagtgagttt ggtttctcgc ggttgggcga
    18121 tctggaacaa gtctttgcgg tcgcgcctca tcatccgtta gccctggaag aagaaccgtt
    18181 aaaccgtcgt atcattaagc gatatcgggc gattgtagta ggagatactg cgcaggcagg
    18241 cgcttctacg gcctcgcaac ttctcgacga acaagaagcc attaccgttt tcgattttaa
    18301 aaccaagctg gagctgcaaa ttagcggcct cggctgcggc tatttacccc gttatctggc
    18361 gcaacgtttt ctcgatagtg gcgcgttaat cgagaagaaa gtggtcgccc aaactctctt
    18421 tgaacctgtc tggattggct ggaacgaaca gaccgcagga cttgccagcg cctggtggcg
    18481 agacgagatt ttagcaaata gtgcgatcgc cggtgtttat gcaaaatctg atgacggaaa
    18541 atcagccatt taaagaaaaa ttattatgac aagcctctca ttctcttgtc atttcccccc
    18601 catttaggca caatgcgccg ctgtcaaaaa atgactaaaa accgacgttt aattagcgtc
    18661 ggttattttt tgcttcaaac caatcattca taccaagagg ccgggcttcg taccggatag
    18721 atatttacta aaaatcgaca gttgttgtcg ctgaggaatc caaaaaaatg gggcaatttt
    18781 ttgcttacgc gacggttatt accgtaaagg agaatgacca tgtcgcataa cgttactcca
    18841 aacacctctc gcgtggaatt gcgtaaaacg cttacgttag ttccggttgt aatgatgggt
    18901 cttgcctata tgcagccgat gacgctgttt gatacatttg gtatcgtttc aggcctcacg
    18961 gatggtcatg tgccgacagc ctatgcgttc gcattgattg cgatcctgtt tacggctctg
    19021 agctacggga agctggtccg ccgctatcct tctgctggct ctgcatacac ttacgcccag
    19081 aaatccatta gcccgactgt cggctttatg gtgggttggt cttctctgct cgactatctg
    19141 ttcgcgccga tgatcaacat tctgctggcg aaaatctatt ttgaagctct ggtgccttcc
    19201 atcccatcgt ggatgtttgt ggtggcgctg gtggccttta tgaccgcctt taacctgcgt
    19261 agtctgaaat ccgtagcgaa cttcaacact gtaatcgtcg tgttgcaggt agtgctgatc
    19321 gcggtgattc tgggcatggt tgtttatggc gtatttgaag gtgaaggcgc gggtacgctg
    19381 gcgagcactc gtccgttctg gtctggcgat gcgcatgtta tcccgatgat taccggggcg
    19441 acaatcctgt gcttctcctt taccggcttt gacggcatca gcaacctgtc ggaagaaacc
    19501 aaagatgcag agcgcgtgat cccgcgtgcg attttcctga ccgcgctgat tggcggcatg
    19561 atcttcatct ttgcaactta cttcctgcag ctgtacttcc cggatatctc tcgctttaaa
    19621 gatccggatg cgtcacagcc tgaaatcatg ctgtacgttg cgggtaaagc gttccaggtt
    19681 ggcgcgctga tcttctccac cattaccgta ctggcgtccg gtatggcggc gcacgcaggc
    19741 gtagcgcgtc tgatgtacgt aatgggtcgt gacggcgtgt tcccgaaaag cttcttcggt
    19801 tatgtacacc cgaaatggcg tactccggcg atgaacatca tcctggttgg cgcgattgcc
    19861 ctgctggcaa tcaacttcga cctggtaatg gctacggcgc tgattaactt tggtgcgttg
    19921 gtggcgttca ccttcgttaa cctgtcggtc atctcgcagt tctggatccg tgagaagcgt
    19981 aacaagacgc tgaaagatca cttccagtat ctgttcctgc cgatgtgtgg tgcgctgacc
    20041 gttggtgcgc tgtgggttaa cctggaagaa agctcaatgg ttctgggtct gatctgggct
    20101 gctatcggtc tgatttacct ggcatgcgta accaagagct tccgtaaccc ggttccgcag
    20161 tacgaagacg tagcataatc atcttttcta ataatcaacc ggagtcgaat gaatatttgg
    20221 ctccggtttt tttattcttt tttatcg
//
LOCUS       DAYPHN010000080        19874 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000080 DAYPHN010000000
VERSION     DAYPHN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.080"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACG0BW_002758"
     CDS             complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACG0BW_002758"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFK7259701.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATAHARELLAKVGLEGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLH"
     gene            complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACG0BW_002759"
     CDS             complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACG0BW_002759"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFK7259702.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACG0BW_002760"
     CDS             complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACG0BW_002760"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFK7259703.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVVDQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRCKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2645..3445)
                     /gene="tcyJ"
                     /locus_tag="ACG0BW_002761"
     CDS             complement(2645..3445)
                     /gene="tcyJ"
                     /locus_tag="ACG0BW_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7259704.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3533..4084)
                     /gene="fliZ"
                     /locus_tag="ACG0BW_002762"
     CDS             complement(3533..4084)
                     /gene="fliZ"
                     /locus_tag="ACG0BW_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFK7259705.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4130..4849)
                     /gene="fliA"
                     /locus_tag="ACG0BW_002763"
     CDS             complement(4130..4849)
                     /gene="fliA"
                     /locus_tag="ACG0BW_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFK7259706.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5169..6833)
                     /locus_tag="ACG0BW_002764"
     CDS             complement(5169..6833)
                     /locus_tag="ACG0BW_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFK7259707.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGEGETANTAATLKDMVGLKLDNTGVTTAGVNRYIADKAVASS
                     TDILNAVAGVDGSKVSTEADVGFGAAAPGTPVEYTYHKDTNTYTASASVDATQLAAFL
                     NPEAGGTTAATVSIGNGTTAQEQKVIIAKDGSLTAADDGAALYLDDTGNLSKTNAGTD
                     TQAKLSDLMANNANAKTVITTDKGTFTANTTKFDGVDISVDASTFANAVKNETYTATV
                     GVTLPATYTVNNGTAASAYLVDGKVSKTPAEYFAQADGTITSGENAATSKAIYVSANG
                     NLTTNTTSESEATTNPLAALDDAIASIDKFRSSLGAIQNRLDSAVTNLNNTTTNLSEA
                     QSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7099..8505
                     /gene="fliD"
                     /locus_tag="ACG0BW_002765"
     CDS             7099..8505
                     /gene="fliD"
                     /locus_tag="ACG0BW_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFK7259708.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKAELFSATSTTSSTTAFSATTAGKAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTVIATSDSKITIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8530..8940
                     /gene="fliS"
                     /locus_tag="ACG0BW_002766"
     CDS             8530..8940
                     /gene="fliS"
                     /locus_tag="ACG0BW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFK7259709.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8940..9305
                     /gene="fliT"
                     /locus_tag="ACG0BW_002767"
     CDS             8940..9305
                     /gene="fliT"
                     /locus_tag="ACG0BW_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFK7259710.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVEPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9383..10870
                     /gene="amyA"
                     /locus_tag="ACG0BW_002768"
     CDS             9383..10870
                     /gene="amyA"
                     /locus_tag="ACG0BW_002768"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFK7259711.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10904..11317)
                     /gene="yedD"
                     /locus_tag="ACG0BW_002769"
     CDS             complement(10904..11317)
                     /gene="yedD"
                     /locus_tag="ACG0BW_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFK7259712.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11504..12709
                     /gene="yedE"
                     /locus_tag="ACG0BW_002770"
     CDS             11504..12709
                     /gene="yedE"
                     /locus_tag="ACG0BW_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFK7259713.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYI
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12706..12939
                     /gene="yedF"
                     /locus_tag="ACG0BW_002771"
     CDS             12706..12939
                     /gene="yedF"
                     /locus_tag="ACG0BW_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFK7259714.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13046..13714
                     /gene="yedK"
                     /locus_tag="ACG0BW_002772"
     CDS             13046..13714
                     /gene="yedK"
                     /locus_tag="ACG0BW_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFK7259715.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFLAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAARKWMRQEIGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPI"
     gene            13825..14305
                     /gene="yedL"
                     /locus_tag="ACG0BW_002773"
                     /pseudo
     CDS             13825..14305
                     /gene="yedL"
                     /locus_tag="ACG0BW_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(14443..15557)
                     /locus_tag="ACG0BW_002774"
                     /pseudo
     CDS             complement(14443..15557)
                     /locus_tag="ACG0BW_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
     gene            15741..15899
                     /locus_tag="ACG0BW_002775"
                     /pseudo
     CDS             15741..15899
                     /locus_tag="ACG0BW_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(15917..16231)
                     /gene="fliE"
                     /locus_tag="ACG0BW_002776"
     CDS             complement(15917..16231)
                     /gene="fliE"
                     /locus_tag="ACG0BW_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFK7259716.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            16446..18104
                     /gene="fliF"
                     /locus_tag="ACG0BW_002777"
     CDS             16446..18104
                     /gene="fliF"
                     /locus_tag="ACG0BW_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFK7259717.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LMAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            18097..19092
                     /gene="fliG"
                     /locus_tag="ACG0BW_002778"
     CDS             18097..19092
                     /gene="fliG"
                     /locus_tag="ACG0BW_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFK7259718.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            19085..19771
                     /gene="fliH"
                     /locus_tag="ACG0BW_002779"
     CDS             19085..19771
                     /gene="fliH"
                     /locus_tag="ACG0BW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFK7259719.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            19771..>19874
                     /locus_tag="ACG0BW_002780"
     CDS             19771..>19874
                     /locus_tag="ACG0BW_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259720.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRAT"
BASE COUNT         4994 a         5055 c         4925 g         4900 t
ORIGIN      
        1 ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagatt
       61 tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt
      121 attaatgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgctga ggatcggcaa
      181 ataacgcttt tgcggccccc tgctcgacta tccgcccctg gtccataaag atcgcccggt
      241 ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct
      301 gcgccagctg acggatggtg ttcagcactt cgcccaccag ctctggatcc agcgccgacg
      361 ttggctcgtc aaacaaaatc acctcaggac gcatagccag cgctcgcgca atcgcgacac
      421 gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttacct tccagcccaa
      481 ctttcgccag cagctcgcga gcgtgcgccg tggcctcttc tttcggttcg cctttgacga
      541 tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct
      601 ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg
      661 aacgtgcagt atcaatggtg atatcgccaa cggtgatggt tcccgcttcc ggttgttcca
      721 gcagatttat gctgcgtagc aacgtggttt tgccggaacc acttggcccg ataattgcca
      781 ccacttcgcc aggcttcacc tcaaggtcga taccgtgcaa caccgtctga ccgtggaatt
      841 ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag
      901 ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc agtagatgag
      961 cgaagccgcc aggtacatgg tgaacacctc cagcgtacgc gaggtaatca actgcgcctg
     1021 acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatctttta ccaggctgat
     1081 aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg
     1141 catggtctgc cacggcgtca taccaatact ggcagcagct tcccactgac ctttatcaat
     1201 agaagaaatg gcggcgcgca gcgtttcggc agcataggcg gcagtgttta gcgacagacc
     1261 aatcattgct gacggaatgg gatctagttc gatgccaaac tgcggcaagc cgtaatagat
     1321 cataaacagt tgggcgatga gcggcgtacc acgaaaaata gagatataaa aacgcgccag
     1381 ccagcgcacg ggccagattg gcgaaaggcg catcagcgcg agaataaacc cgagcagtaa
     1441 gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt tcagcaggaa
     1501 cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgtttccg gagctaaacg
     1561 tggggatgat aggcgaacag cgcaggcgcg ccgccggtat gaataaacag aatcggccct
     1621 tcatctttgc agcgtttctg actgataccg tcaatcagac ccgccatcgc ttttccggta
     1681 tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct
     1741 tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa aatttccgct
     1801 gatgcggtca gctccagttc tttcgcaatc gcctgttgga ggttaaccac tttcggcaat
     1861 tgatcgacaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt
     1921 tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgacgcgac caccaccgac
     1981 gaaatattaa ctgccccttc acactgttgc gcaatttcca gcgcactctc cacataaccc
     2041 aatgcgccca gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct
     2101 tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc gtcgcacatt
     2161 tcaatctggg tattgaacag atccagcaac aaacgattgc cgttggttaa atagttttct
     2221 gcggttgtgc caattggatt ttccagcagc gccacgcagt gcagaccgag tttcgccgca
     2281 actgcggcag tctggcgcac atggttagac tggatcgccc cggcagtaat cagcgtatcg
     2341 gcaccttcgc gcagagcatc tgccgcgaga aattccagct tacgtaattt attgccgccc
     2401 attgccatgg gagtgacgtc atcccgtttg ataaaaattt cccgtccaag ataatcagaa
     2461 aagcgcggca gatattcgag cggcgttggc gcgccgataa actccagccg tggaaaacgg
     2521 gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg
     2581 cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt tgtcattatg
     2641 ctgattattt ggtcacatca gcaccaaacc atttttcgga aagggcttgc agagtgccat
     2701 ctttttgcat ttccgcaatt gcatcattca ccgctttcag caggtcttca tttcctttac
     2761 gcagcgccac accagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt
     2821 tggttttctt caccagatcc agcgccgcca gacggtcaac gaggatcgca tcgatacggc
     2881 ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacgccct
     2941 gaacattctg acgcagccac tcttcatagt tggtgcccag accgacaccc acttttttgc
     3001 ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa
     3061 taccggaaat ggtgtacggg gtagagaaat cgtatttttt cttgcgctcg tcagaaatgg
     3121 tgacctgatt aatcaccaca tcaatgcgtt tagagtccag cgacgccagc ataccgtccc
     3181 atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt
     3241 ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagttc
     3301 cttccagccc taccagcagc gtgccgcgct ctttgacttt attaagcaga ccttcatctg
     3361 caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc atcaatgcct
     3421 gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct gtttgcagtg
     3481 tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt atttaatata
     3541 tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacacgtt tgacgctgat
     3601 agtgttgata cttccgtaac gcaatgcggt aattgttggt gctggtggtg ggcagccacg
     3661 gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata ttttgctcgt
     3721 gtagatgatt acccaaacgg cgcagacgaa cgacatattc acgcaccgta cctgggctca
     3781 tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa tcactctgcg
     3841 ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgatttt tgttccactt
     3901 gccagccatt ttcatcaagc agcatgtcca ggcgggaaat ctcgatttta ttcactattt
     3961 tgccgtcgcg aacgagggta atgcgatcga gtaatttacg gcaatgcgcg caatgggtct
     4021 ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca
     4081 tcattaagaa ctcctggtag tcaaaattaa agtgcggcat ttactgacgt tataacttac
     4141 ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc cgcgattccc
     4201 cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta
     4261 ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc catcacccgc tggcgcagat
     4321 tactgtccag cagctgttgt agcgggtttt ctcgctgatg atcatcagta accagttcaa
     4381 tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg ttattggtgt
     4441 cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct acctcagttt
     4501 ccgtggcgtt acggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg
     4561 cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc
     4621 cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt
     4681 cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg
     4741 cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc
     4801 acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc
     4861 ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta atcggacgat
     4921 tagtgggtga aatgaggggt tatttggggg ttaccagtaa attgcggaca gaaaaaaccc
     4981 cgccggtggc ggggaagtac gttgctgaca aattacgctt catgttgccg gatgcggcgt
     5041 aaacgtcttt tccggcctac aaaaattagc aaattcaata aattgcaatt caccttgtag
     5101 gcctgataag cgcagcgcat caggcaattt ggcgttgccg tcagtctcag ttaatcaggt
     5161 taggacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt ggcttttgcc
     5221 agcacggagt taccggcctg ctgaatgatc tgcgctttcg acatgttgga cacttcggtc
     5281 gcatagtcgg cgtcctgaat acgggactgc gcttcagaca ggttggtagt ggtgttgttc
     5341 aggttggtga ctgcggaatc cagacggttc tggatagcac ccagggaaga acggaatttg
     5401 tcgatagacg cgatagcgtc atccaatgct gccagcgggt tggtagtagc ttcagattca
     5461 ctagttgtat tagtcgttaa gttaccattg gcacttacat agatagcttt actggtagcc
     5521 gcattttcac cactagtaat agtgccatca gcttgagcaa aatactcggc aggagttttg
     5581 ctcacttttc catcgactaa atacgctgat gcagcagtgc cattattgac tgtatatgtc
     5641 gcaggtaaag ttacaccaac agttgcagtg taagtctcat ttttaacggc gttagcaaac
     5701 gttgaagcat caacagaaat atctacccca tcaaactttg tcgtattagc agtaaatgta
     5761 cctttatctg ttgtaatgac tgttttggca ttagcattgt ttgccattaa gtcagacagt
     5821 ttagcttgag tatcagtgcc tgcgttagtt ttacttaagt taccagtatc atcaagatag
     5881 agagcggcac cgtcatcagc agcagttaaa gaaccatctt tagcaataat gactttttgc
     5941 tcttgagctg ttgtaccgtt gccaatactt actgttgcag cagtggtacc acccgcttca
     6001 ggattcagga atgccgccag ttgagtcgca tcaactgaag cagaagccgt atatgtgtta
     6061 gtatctttat gataagtata ttccactggc gtaccagggg cagctgcacc aaaaccaaca
     6121 tctgcctccg tggaaacttt actgccatca acaccagcta ccgcattcaa aatatccgtg
     6181 ctacttgcga cggctttgtc agcaatatat ctattaactc cagctgtagt gacccccgta
     6241 ttatcgagtt ttaaaccaac catatcttta agtgttgcag cagtattggc tgtttcacct
     6301 tcgccattca cgttaaaacc agccagattc agcgtatcag agtcaatttt cttcagatca
     6361 atcgtgatgg tttcaccatc atttgcgccg acctgaattt tcattgaacc atctttggac
     6421 agcacgttca cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtccagacga
     6481 gatttgattt cgtcctggat ggagtccagg tcagaatcgg agttagtccc tgtagtggcc
     6541 tgaaccgtca gttcacgaat acgctgtaag ttgttgttga tttcggacag cgcgccttca
     6601 gtggtctgtg caacagaaat accgtcattg gcgttacgtg cagcctgagt caggccttta
     6661 atattagaag taaaacggtt agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta
     6721 atacgcaagc cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg
     6781 atattatttt gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt
     6841 tatcctatat tgcaagtcgt tgattacgta ttgggtttcc acccgtcggc tcaatcgccg
     6901 tcaaccctgt tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt
     6961 ttttgttagt cgccgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca
     7021 attaaacgta aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga
     7081 aagataaaag gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggcctggat
     7141 ttaagttcaa tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca
     7201 aatcagcaat cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg
     7261 acgactttcc agaccgccaa tactgcattg tctaaagccg aacttttttc cgccaccagc
     7321 accaccagca gcaccaccgc gttcagtgcc accaccgcgg gtaaggccat cgccgggaaa
     7381 tacaccatca gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga
     7441 gatgatacga aaacggtgat cgccaccagc gacagtaaaa tcaccattca acaaggcggc
     7501 gacaaagatc cgattaccat tgatatcagc gcggctaact cgtcgttaag cgggatccgt
     7561 gatgccatca acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt
     7621 gaatatcgtc tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg
     7681 gtcagcggtg atgatgcgct acaaagtttt atgggctatg acgccagtgc cagtagcaac
     7741 ggtatggagg tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatt
     7801 gagaacagca gcaacaccat cagcgacgcg ctggaaaaca tcaccctgaa cctgaatgat
     7861 gtcaccacgg gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg
     7921 attaaagact gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa
     7981 tacaccgccg tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc
     8041 gactccacgc tgcggacgat tcagacgcag ttgaaatcga tgctgagtaa taccgtcagt
     8101 tcttccaact ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa
     8161 ctggaactgg atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt
     8221 gcattgattg ttggcgatgg taaaaaaacc ggcatcacga ccaccatcgg cagcaacctg
     8281 accagttggc tttcgacaac aggcattatt aaagccgcta ccgatggcgt tagtaagacc
     8341 ctgaataagt taactaaaga ctacaacgcc gccagcgatc gcattgatgc acaggtcgct
     8401 cgctacaaag aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc
     8461 agctacttaa cgcagcagtt cgaaaacaac agtaattcca agtaatcaat attcatcggg
     8521 agacaggtca tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc
     8581 gccgtaatga gcgccagcca acagcagctg gtcaccatgc tatttgatgg agtgctgagc
     8641 gcactggtta gagcgcgcct gtttatgcag gacaacaatc agcaaggcaa aggcgtctct
     8701 ttgtcaaaag cgatcaacat cattgagaac gggctgcggg tgagtcttga tgaagagagc
     8761 aaagacgaac taacccaaaa cttgattgct ctttatagct atatggtcag gcgcttgctg
     8821 caagccaatt tacgcaacga tgtctccgca gtcgaagaag tggaagcatt aatgcgcaat
     8881 attgccgatg cctggaaaga gtctttactc tccccttctt tgattcagga cccagtctga
     8941 tgaacaatgc accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca
     9001 tgttacgcct ggcaacggaa gaacaatggg acgaactcat cgccagcgaa atggcgtatg
     9061 tgaatgcggt gcaggagatt gcacacctga ctgaagaggt tgagccgtcc accacgatgc
     9121 aggagcaact ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt
     9181 tattacagat tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat
     9241 cggtgttaag tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct
     9301 tttgaatctt aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatgt
     9361 tccccttgaa tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta
     9421 ttacccggaa ggcggtaagc tctggcctga actggccgag cgcgccgacg gttttaatga
     9481 tattggtatc aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcgggtattc
     9541 ggtcggctac gactcctatg atttatttga tttaggcgag tttgatcaga aaggcagcat
     9601 ccctactaaa tatggcgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa
     9661 tgacattgcg gtgctgttgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga
     9721 agctattcgc gtgcaacgtg taaatgctga tgaccgtacg caaattgatg aagaaatcat
     9781 tgagtgcgaa ggctggacgc gttacacctt ccccgcccgt gccgggcaat actcgcagtt
     9841 tatctgggat ttcaaatgtt ttagcggtat cgaccatatc gaaaatcctg atgaagatgg
     9901 catatttaaa attgttaacg actacaccgg cgaaggctgg aacgatcagg ttgatgatga
     9961 actcggtaat ttcgattatc tgatgggtga gaatatcgat ttccgcaatc atgccgtgac
    10021 ggaagagatt aaatactggg cgcgctgggt gatggaacaa acgcagtgcg acggttttcg
    10081 tcttgatgcg gtcaaacata ttccggcgtg gttttataaa gagtggatcg aacacgtaca
    10141 ggaagttgcg ccaaagccgc tgtttattgt ggcagagtac tggtcgcatg aagtcgataa
    10201 actgcaaacg tatattgatc aggtggaagg caaaaccatg ctgttcgatg caccgctgca
    10261 gatgaaattc catgaagcat cgcgcatggg gcgcgactac gacatgacgc agattttcac
    10321 gggtacatta gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac
    10381 ccaaccgttg caagccctcg aagcccccgt cgaaccgtgg tttaaaccgc tggcgtatgc
    10441 tctaattttg ttgcgggaaa atggcgttcc ttcggtgttc tatcctgacc tctatggcgc
    10501 gcattacgaa gatgttggcg gtgacgggca aacctatccg atagatatgc caataatcga
    10561 acagcttgat gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacgttatt
    10621 tttcgaccat ccgaactgca ttgcctttag ccgcagtggc actgacgaat atcccggttg
    10681 cgtggtggtc atgtcgaacg gggatgatgg cgaaaaaacc attcatctgg gagagaatta
    10741 cggcaataaa acctggcgtg attttctggg gaaccggcaa gagagcgtag tgaccgacga
    10801 aaacggcgaa gcaaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga
    10861 ggtgatttaa attcatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg
    10921 gtaacggcgc tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca
    10981 tcgtcatacc atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt
    11041 ttacggtaac gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acagcaatca
    11101 cccgctgcca ctgacggcaa tccagagtat cgccctcttt agtgacaatc aggctgccta
    11161 tcgcttccgg actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc
    11221 agtccggcgc gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca
    11281 gcaacatgag cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa
    11341 agaggattgt ggcattaaag ccctgatgtc gccagccttt ccaggttcag agagaaattt
    11401 gatctaacta ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt
    11461 cgcgactcct gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt
    11521 caaacacgcc tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat
    11581 tctctccact tactattttg gcatcactgg caccttttgg gctgtcacgg gtgaatttac
    11641 ccgttggggc ggtcagctcc tgcagctgtt cggcgtccat gctgaagagt ggggttattt
    11701 taaaattatc catctggaag gatcgccatt aacccgcatc gacgggatga tgatcctcgg
    11761 tatgtttggc ggctgctttg ccgcagcgct gtgggccaac aatgtcaaac tacgcatgcc
    11821 gcgcagccgt atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc
    11881 gcgtctggca atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct
    11941 gcatgcctgg ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac
    12001 cctgctgccc atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct
    12061 gacgcaaaaa ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg
    12121 catgctgggc tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct
    12181 gtttggcgtc ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt
    12241 ccgcgatatg tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc
    12301 ggtaagtgcc atcgggatct tcagttacat acagttaggc gttgaaccta aaatcatgtg
    12361 ggcgggacca aacgcggtaa ttggtggttt actgtttggt tttggcatcg tgctggcagg
    12421 cggctgcgaa accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt
    12481 cggtctgggc aatgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc
    12541 ggcgctggcc accgactggg acaaaatcaa cctgctaaaa acctttggtc cgatgggtgg
    12601 cctgctggtg acatatttgc tgttgtttgc tgcgctgatg ttgattatcg gctgggaaaa
    12661 acgcttcttc cgccgtgcgg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt
    12721 cctgattacc gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt
    12781 gaggcgatgc cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag
    12841 tcgatcaata atattccact ggatgcgcgt aatcacggct atacggtgct ggatattcag
    12901 caagacgggc cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc
    12961 aacagcggga acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt
    13021 ttcagtatga tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga
    13081 agattacctt gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc
    13141 cattggcaga tacaacgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga
    13201 acaccttcat ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc
    13261 gctgattaac gcccgcgtag aaactgccgc aaccagtcgc atgtttaaac cgctctggca
    13321 acacggtcgg gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa
    13381 aaagcagcct tattttatct atcgtgctga tggacaacct gtttttctgg ccgcgatagg
    13441 cagcacaccg ttcgagcgtg gtgacgaagc cgaaggattt ttgatcgtca ctgcagcagc
    13501 agaccaaggt ctggtagata ttcatgaccg ccgcccgctg gtactgtcgc cagaagctgc
    13561 gcggaaatgg atgcggcaag agattggcgg taaagaagcc tctgaaatcg ctaccagtgg
    13621 ctgtgtgcca gcaaaccagt tcacctggca cccggtatcg cgcgcggtgg gtaatgttaa
    13681 aaaccagggc gcggagttaa ttcaacctat ctgataagcc tggagattat tgatcaacaa
    13741 tactgcgtca taagaaatct ctattagaca aagatttcag tacctgttgg catattgcaa
    13801 aaataacacc aatacggaat cgtcatgttc acgattaaaa cagatgatct cacccatcca
    13861 gcagtgcaag cattagtggc ttaccatatt tccggcatgc tgcagcagtc tccccccgaa
    13921 agcagtcatg ctttagatgt gcaaaaatta cgtaacccga cagtgacatt ctggtcagta
    13981 tgggaaggcg aacaactcgc aggaattggt gcgctgaagt tgctggatga taaacatggc
    14041 gaactgaaat caatgcgtac cgcgccaaat tatttacgtc gaggtgtcgc cagtctgatt
    14101 ttacgccaca ttttgcaggt cgcccatgac agatgccttc atcgcctgag cttagaaacg
    14161 ggtacacagg ctggatttac ggcctgccat caactttatt tgaagcatgg ttttcgttga
    14221 ttgcgaaccg tttgctgatt atcaacttga tccacacagt cgatttttgt cattgacgct
    14281 atgcgaaaat aatgaattgc catgagccag acgcagcaca ttcttgcatt cgacgtgctg
    14341 cgcctttatt catcaccagc cgcatatgtc ttgtccttag acgctccttc cacatgcgcc
    14401 acaagaaacc tctattccag tgacacaatt gcgcctaact atttaattct taccatctat
    14461 tacgcccctt ttgactatgt cagaatattt atcataatca gcggaattcg taacaatata
    14521 ttttcgctgc gatatttcat aggtaatgtc tgtcaaagtc cacggcatta aaaactcctc
    14581 tttaatagga taacattcta taaaaagtaa ttttaaattg ctatgtatcg cagggacaac
    14641 ctcaatatta tactccatac gtgctgtctc cagatgattt gggaagtatt gaattttttt
    14701 atcttattat caccacaatt aaacagatta gtagtctcag gcattgctgg tatttaccat
    14761 attttattgc gataaataaa aattttcata gcctttgacg gagatgagtc aacacgcgat
    14821 atcagattat tatctacgga gacctgggta attccatcgg atacttttgg aaataccata
    14881 taattattaa aatcgagttc taattctttg cagaatttgg gaatttgtat tgtggcaagg
    14941 ccattatgac gaagagaaag cacctcaatc cagtctggaa gaggtggcaa agaccccaag
    15001 tgaagatctg ttaagtcaag tttactattg tggagttcaa cacatttaaa caatctcacc
    15061 aatgctatat ccacattttc tttgaagtca ttcgatgcgc cttctttgac tgaattagcc
    15121 cagtcactga aacctttgca aagtctggca tctgtaattt tcgttaactt gtctagaaac
    15181 tctgcaatat tagtcgatat aaactctaat gtatgccgaa tgggtggatt accagctccc
    15241 tgactttttt ctatcatgat aaaacctgtc ttatcataaa tcgagaaacc ttgaaaccag
    15301 agactataat caccccattc gaaatattct gagtgcaata acttaaattc tgacagcata
    15361 ttaaataatt tgctgtcttc actaattggt gtaaacttac catttccaag acaactgact
    15421 aactcacttg ataattcatt tatgattgca tcatcagata ctaactcgtg ttgttttatc
    15481 gtagattcag tattagatgt gactttattt aattgaaccg gctgagtttt taacgaaaga
    15541 tcatttaatg gaaacattta cactcctgtt taataaatta tattgcccag ggagcattct
    15601 aaaacataat aaaattacca ctatcaaaaa tcgctcattt ttataacgca gtatattaat
    15661 aaaaataaga acgaagatta aattatccag aggttactgt tgcgtctgtg gtcgtaacaa
    15721 catccgacgt ggtgatgtgt caaatgagac actctgatgg tcaaagggtt tattgcgttt
    15781 acggatcgtg gatggcagaa aacgttcaga atcaggtttc cattttaaac cagaaattga
    15841 gtgtgtttgc cccatccatg ccccacgcgg tgagatcgga tgtaattaaa caagcatagt
    15901 caacaggtta cacaacctac acctgcatgc tcatcacttc ctgatacgcc gccaccagct
    15961 tattacgcac ctgaatcccc atttgcattg aaactgaggc tttttgcata tcggtcatca
    16021 catcgtttaa cgccacgccg ggttcaccga gagtgaattt ttctgcctgc gtgcgggcgg
    16081 ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg
    16141 tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtggtctgt aactggctga
    16201 taaccccttc aatcccctgt atcgctgaca ttttcatctc ccgcataatt tctggtagca
    16261 aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa acgggtttat
    16321 tggcggatag aaaaaaacga aagcacaaat aatgggagcg tcaatttttc gagtttgctg
    16381 acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca gccacgaggt
    16441 gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat cgcctgcgtg
    16501 cgaatccgaa aattccattg attgttgccg gttccgcagc agtggcggtc atggtcgcac
    16561 tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt tccgatcagg
    16621 atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc ttcagcgaag
    16681 ccagcggcgc tattgaagtt ccggcagata aagttcacga gctgcgtctg cgcctggcgc
    16741 aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact gctcgatcag gaaaagtttg
    16801 gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc gagctttctc
    16861 gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca atgccgaaac
    16921 cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta aatctgttac
    16981 ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcatctggtt tccagtgcgg
    17041 ttgctggtct gccgccaggg aacgtcacgc tggtggatca gggtggacat ctgttaaccc
    17101 agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc agcgatgtcg
    17161 aaggccggat tcagcggcgt attgaagcga tcctgtcgcc aattgttggt aacggtaata
    17221 ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg gaagaacagt
    17281 atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt aatgagagcg
    17341 agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat caaccggctc
    17401 ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc cagcagcagg
    17461 cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc agtaactacg
    17521 aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtccg
    17581 tcgcggtcgt ggtgaattac aaaaccttgc cagatggcaa accgttgcct ctcagcaacg
    17641 aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat gggcttttct gaaaaacgcg
    17701 gtgattcgct caatgttgtt aactcgccgt tcaatagtag tgatgaaagc ggtggagaac
    17761 tgccgttctg gcaacagcaa gcgtttatcg atcagttaat ggctgccggt cgctggttgc
    17821 tggtactgct ggtggcgtgg ctgctgtggc ggaaagcggt acgcccgcag ctaacacgtc
    17881 gcgctgaggc gatgaaagct gtgcagcaac aggcgcaggc ccgtgaggaa gtggaagatg
    17941 cggtggaagt ccgcctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac
    18001 tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg
    18061 tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat
    18121 aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaaacac
    18181 ctttcccagc gcgaagtgca aactctgagc gctgcaatgg cgaacgtcac gcagatctcc
    18241 aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgccgca
    18301 ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa
    18361 cgtgccgcca gcctgctgga ggatattctc gaaacccgcg ataccgccag cggtattgaa
    18421 acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatcctcaa
    18481 attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat tctggcgttg
    18541 ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacttttgg tggcgtgcag
    18601 ccagccgcgc tggcggaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc
    18661 aagcgcagta aaatgggcgg cgtgagaaca gcagccgaaa ttatcaacct gatgaaaact
    18721 cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa
    18781 atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgacgatcg cagcattcag
    18841 cgactgttgc aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg ggccgagcaa
    18901 ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac
    18961 gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca gaaagcgatt
    19021 ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag cggcgaggat
    19081 acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccaca
    19141 ggcagagttt gtgcccatgg tcgagccgga agaaaccatc attgaagagg ccgaacccag
    19201 ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcaaggtt atcaggcggg
    19261 gattgccgaa ggtcgccagc aaggtcatga gcagggctat caggaaggac tggcccaggg
    19321 gctggaacaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg cccggatgca
    19381 gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga ttgcttcgcg
    19441 cctgatgcag atggcgctgg aggcggcacg tcaggtgatc ggtcagacgc caacggtgga
    19501 caactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg
    19561 taaaccacag ctgcgcgtgc acccggatga tctgcaacgt gtggatgata tgctcggcgc
    19621 aaccttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccatc ctggcggctg
    19681 taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact
    19741 ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc
    19801 acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga
    19861 ttaactcgcg ctac
//
LOCUS       DAYPHN010000081        19865 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000081 DAYPHN010000000
VERSION     DAYPHN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.081"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(34..687)
                     /locus_tag="ACG0BW_002781"
     CDS             complement(34..687)
                     /locus_tag="ACG0BW_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFK7259721.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(1278..1975)
                     /locus_tag="ACG0BW_002782"
     CDS             complement(join(1278..1726,1726..1975))
                     /locus_tag="ACG0BW_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFK7259722.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(2031..2261)
                     /locus_tag="ACG0BW_002783"
     CDS             complement(2031..2261)
                     /locus_tag="ACG0BW_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259723.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMLLFLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            complement(2287..2850)
                     /locus_tag="ACG0BW_002784"
     CDS             complement(2287..2850)
                     /locus_tag="ACG0BW_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFK7259724.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2898..4259)
                     /locus_tag="ACG0BW_002785"
     CDS             complement(2898..4259)
                     /locus_tag="ACG0BW_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFK7259725.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            complement(4311..4541)
                     /locus_tag="ACG0BW_002786"
     CDS             complement(4311..4541)
                     /locus_tag="ACG0BW_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259726.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(5576..5767)
                     /locus_tag="ACG0BW_002787"
     CDS             complement(5576..5767)
                     /locus_tag="ACG0BW_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259727.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(5764..6186)
                     /locus_tag="ACG0BW_002788"
     CDS             complement(5764..6186)
                     /locus_tag="ACG0BW_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFK7259728.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(6233..6658)
                     /locus_tag="ACG0BW_002789"
     CDS             complement(6233..6658)
                     /locus_tag="ACG0BW_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFK7259729.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(7075..7851)
                     /locus_tag="ACG0BW_002790"
     CDS             complement(7075..7851)
                     /locus_tag="ACG0BW_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259730.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(7902..8420)
                     /locus_tag="ACG0BW_002791"
     CDS             complement(7902..8420)
                     /locus_tag="ACG0BW_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFK7259731.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDAPLMLVIWSPEEIQALADGMEISLTDHEIRTVLARMEDIPEDQRTESGISSAAVM
                     EIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RTLLKNNTHENN"
     gene            complement(8350..8571)
                     /locus_tag="ACG0BW_002792"
     CDS             complement(8350..8571)
                     /locus_tag="ACG0BW_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259732.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(8572..9255)
                     /locus_tag="ACG0BW_002793"
     CDS             complement(8572..9255)
                     /locus_tag="ACG0BW_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFK7259733.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(9332..9643)
                     /locus_tag="ACG0BW_002794"
     CDS             complement(9332..9643)
                     /locus_tag="ACG0BW_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259734.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARQERCLWSVSRV
                     RWARDGRFYCSRLAPVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(9640..10542)
                     /locus_tag="ACG0BW_002795"
     CDS             complement(9640..10542)
                     /locus_tag="ACG0BW_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFK7259735.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(11409..12380)
                     /locus_tag="ACG0BW_002796"
     CDS             complement(11409..12380)
                     /locus_tag="ACG0BW_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFK7259736.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(12380..13546)
                     /gene="sopA"
                     /locus_tag="ACG0BW_002797"
     CDS             complement(12380..13546)
                     /gene="sopA"
                     /locus_tag="ACG0BW_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFK7259737.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(14134..14889)
                     /gene="repE"
                     /locus_tag="ACG0BW_002798"
     CDS             complement(14134..14889)
                     /gene="repE"
                     /locus_tag="ACG0BW_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HFK7259738.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(15663..16469)
                     /locus_tag="ACG0BW_002799"
     CDS             complement(15663..16469)
                     /locus_tag="ACG0BW_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFK7259739.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(16470..16775)
                     /gene="ccdB"
                     /locus_tag="ACG0BW_002800"
     CDS             complement(16470..16775)
                     /gene="ccdB"
                     /locus_tag="ACG0BW_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFK7259740.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(16777..16995)
                     /gene="ccdA"
                     /locus_tag="ACG0BW_002801"
     CDS             complement(16777..16995)
                     /gene="ccdA"
                     /locus_tag="ACG0BW_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFK7259741.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            17703..18698
                     /locus_tag="ACG0BW_002802"
     CDS             17703..18698
                     /locus_tag="ACG0BW_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFK7259742.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            18702..19634
                     /locus_tag="ACG0BW_002803"
     CDS             18702..19634
                     /locus_tag="ACG0BW_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="HFK7259743.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
     gene            complement(19771..>19865)
                     /locus_tag="ACG0BW_002804"
     CDS             complement(19771..>19865)
                     /locus_tag="ACG0BW_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFK7259744.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         4850 a         5412 c         5019 g         4584 t
ORIGIN      
        1 tttaacgcac aatgtttaat aaaaatataa ctttcaacca aacagtaaac ccagcgtggt
       61 tgcttccata tgcagcaata ccggcagcag caggccacca cttctgatcc tggccactga
      121 tgtaatcaac cccaccagga acagttctgc cagtgtcagc aggttctgat actggctgtg
      181 cgcggcgacg aacaacaacg acgttatcag cgcccccagc cacatcgtcc agcagtaccg
      241 tgaacggaag acgttcagca taatcccccg gaacagcgtt tcctcattca acggggcaag
      301 gataaagatg gtcagcaacg tcaggatcac gtcaggtatg gacttatcgg caaaaagttt
      361 cgtcataaat ggctcagcag gcagagccag cgccttaccg agcagaaata caccgacata
      421 caccacggcc atcgcaccga ccagccacgg tacgccaacg ttgcgcagct gaccaacgac
      481 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac
      541 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc
      601 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaactg
      661 cagatattga ttacgtgttt gaatcattgc atcgcctgta aatttttaac ttgtcctatt
      721 tttgtcatta ccacgtatat acacatgtat aacaattcag atatcgttac caggatatgc
      781 cgcatcagcg gcatggaagg cggcactctg ttgtttcata tgatacagga gtaaaaccgc
      841 cgaagcccgg cgtaagccgg tactgattga tagatttcac cttacccatc cccagccctg
      901 ccagaccata cccgctttca gccatgagag agcttctgtg cgcggtcgga gtggtcccga
      961 cgagggttta cccgaagtcg gggcgtatct ccgcgttagc gggccgtgag ggccgcttac
     1021 gagcgtgtac tgagaacttc cagcgagaag actgacagcg atgaagatgt agttacaaca
     1081 ttcataatta aaagcgactc tgttccggcc cgaagggccg gggcggggcc gcttttcagt
     1141 tatgagggag gggctttgtg gtttcagttc tgcgctggtt cggggttttt ctggaggttg
     1201 gttttgtgtg ttgtaactaa agtggctccg gttggggccc gccgtttacg gtgggaggtg
     1261 caggtaatga ctccaactta ctgatagtgt tttatgttca gataatgccc gatgactttg
     1321 tcatgcagct ccaccgattt tgagaacgac agtgacttcc gtcccagcct tgccagatgt
     1381 tgtctcagat tcaggttatg tcgctcaatg cgctgagtgt aacgcttgct gattacgtgc
     1441 agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca taccacgacc
     1501 tcaaaggccg acagcaggct cagaagacgc tccagtgtgg ccagagtgcg ttcaccgaag
     1561 acgtgcgcca caaccgtcct ccgtatcctg tcatacgcgt aaaacagcca gcgctgacgt
     1621 gatttagcac cgacgtagcc ccactgttcg tccatttcag cgcagacaat cacatcactg
     1681 cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg
     1741 tgttgaggcc aacgcccata atgcgggcgg ttgcccggca tccaacacca ttcatggcca
     1801 tatcaatgat tttctggtgt gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc
     1861 atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcagtactt ttaccgttac
     1921 gcaccacgcc ttcagtagct gagcaggagg gacaactgat ggagatggaa gccacgggag
     1981 cacctcaaaa acaccatcat acactaaatc agtaagttgg caccattacc tcagagcagc
     2041 aggaaaaaga agaacagtag cagtagcagt agcagtagca gtagcagtag cagtagcagt
     2101 agcagtagca ggaacaacaa catcagtaac agacaaatga caggcatgaa gaaacagaag
     2161 cccccatcag aacgtccgcg taatgaaagg ggcggcgcgt gagcgcggcc cctttcagcg
     2221 ccaccggcgc gcgttttttc ccggcgcggg cagacaggca tttatcctcc cgccggccct
     2281 gtgtcatcag gcacgcagac gaataatcat cgtcgggaca tcggtataag caaacgtgcc
     2341 gcgcggcagc tcctcgcgga cgtcagaaaa cggtaacagc ttctcctgct ggcgtggtcc
     2401 gttgagacag acggcgacca gcacgccacc cggacgcaac agggaaaagg cccgcaggat
     2461 atgccggata tcctgcccgt ggctgaacgg cggattcatg ataacccggc tgtaatactg
     2521 caccggcagc cattccatga agtcgccaca ctgaaccctg acaccgttaa aattttcccg
     2581 cagataccgg accagcccgc tgttgatttc caccgcatca cacatggccc ccggcgcggt
     2641 atcccgaatc gcccgcagta tggccccggt gccggcagag ggttccagaa tgtgatcacg
     2701 gttgctgatc tccgccagca tcaccaggcg atcacagacc ggggcaggag tcacaaaaag
     2761 ctgagattca gcccggattt cagcaaccgg cgcggcgtta tccagccttg cgcgcatcag
     2821 ccggatacgt tctcttaact gactgtgcat atcattatct ccgtcatccc ccggggattt
     2881 tcccgggggg atatacctta tttctgtggg atttccgtga ttttcatgtc ggagatatac
     2941 acattcacca ggcggaaatt attgtccatt gtgcggcggt accggtatgc cccgtggtct
     3001 tctgcttctg ccacactgcg cacggcctta cagtcccggg gcagggcggc ccactgtgcc
     3061 tttgtcattt cccggaaacc ttcccccgga tagttgacta ccggcggacg cttcgcggcc
     3121 tgactggcga cggcagcctc ttctgccgat ggtgctttgt agtccgtgat gcgatcgggc
     3181 gtcactttca tcgtgccgct gtacccgaga aaactgtaat ttggcgttgt gactgaactc
     3241 accgccccgt tgcttttgtt cacacggatg atggtcagcc actcgccccg gctgaaaacc
     3301 tgtccgcccg gctcaaaatc ctgtgtccgg gtaaccacgc cgccgctttc gtccagcatg
     3361 gcacgctcat agatcaggcg gttctgatag tggttaaccc agcgttgttg atgctgaatc
     3421 tgccgttcat gacagcggat cgcaatttcg cgggcctgct ccgtggtgat gatgtcatca
     3481 tccagggccg accataacga tcgacttcct tcatactggc ttttttctgc cgggcgcgga
     3541 tacgtatcca gcgggaaaca ggcgctgata tggtcatgac tgctgatgag ttttgccata
     3601 ttcagatcca gcgattcagc ccgccacatc gtcagatatt tctgcgactg cgcgatggtc
     3661 ttttcagcct tgcgcaaatc agcttcgatt tttttgatac gacgccagcg aacgtccgga
     3721 cgttctttat acttcgcgtg aagcagtgcc gaccgcgccc gctcttccca gtattccgca
     3781 cgttcaaaga gcatcacggc tcgtttcatg ccgttttcaa tacgctgcgc atcacgacgg
     3841 gcgcggcgtt cgctgtgatg ccccacaaga atgggctgac cgggcgggat catcgcggcc
     3901 agtctttcca cttcatcaag tgcctgtgcg gattcactgg cccgctttcc gctgtatccg
     3961 gtaaaccgct ccgcccgtgc ttcctgacgt tcagcgagcg tgctgtcttc atcctcaatc
     4021 tctccggcaa gtgagaggag cacgtcttcc cggcccggtg tccaggctgg cgcgacaaac
     4081 agcgcctgtt ttggtgccca gcggaaaccg gcatcatgca cttttttata cgtctccggg
     4141 tcaagacgtg acacggcata caggcgcagt ttgttgtcat ccggtgagta ggtcgcgcgg
     4201 tacatctgca cagggctggc ggcttccggg gcggtcatgg tgattgctgc taatgtcata
     4261 aaatgttctc cttccgtctg ttgccgcccc tcgcggggcg gctcacctca tcagttaaaa
     4321 gcgcgtttca gttcgagtgt gcggagcacc tgcccgaagg ccgtgttttt catggcgggg
     4381 atgtgtacac cacgcccccg gtgaaaactc cgggcaatgt gtcgcccgaa ggctttcagc
     4441 acggggtcaa tttccggcgc ggggatgtct tcgcgtctga aacggggatg acggcggggc
     4501 tttacagccg ctgcggaagg tgaaacaata cttactgtca taatttagcc tcttatgacg
     4561 ttaattaatg aaaccgtaaa gactgttcgt gcagtctctg cggtgtggat taagcaggtc
     4621 cctgacctgc ttttttcata tctgcgtctg acgttctccg gtgttttcct ccttctgttc
     4681 ctgcgttctc attacctgcc agccggatga ctgacagaac acataaatgc gtattgccgg
     4741 agcctgaaat aaatcctgat ttttaaagaa catcacccgt tgagtttcgg gagccgcggt
     4801 tttgacgttt cgcgggaacc tgctgctgta taccctgtgg tatgaacttc acccgtttga
     4861 gaaccttcgc cgcaaggggc atcactgcca cggcctgacg cacctgtcct gggcagaacg
     4921 caaaattttt taccgggttt tgggtaaaaa attttgtggg ctgttcattt accctggaca
     4981 gtcagtgcgg caggttgtgg ctgtatcagc cccaggcgac agggactcat acgggtgtgt
     5041 ggcaccggca ggcaatgagc agggacccgc cgcaacgacg gagagcgcgt gcgcgaaacg
     5101 gcgttgcccc ttacatcccg gcggcctttt gccgggatgc cgggagcgca ggccacggcg
     5161 cgacaagggt ggcgcgaccg gaacggtcgc tgcaagcccg gggttttcac gcatcggacc
     5221 gtagcgacag cccgaaaccg cgccagcggg ttcggcggag actggcgggt gcgccagcag
     5281 ctgcctgtcg gagtcgggcc tgacgaccgg atgccgccag gcagccggga gccgaaggtg
     5341 cgcccggcag acagaacaac actgcagtag tcagaatcag agcagcagat aaaggaacaa
     5401 caacatcagg aacagttcca taacataaga aagaaacact cctgagtcag acacatcaca
     5461 gacgggaagg gtaacgggcg acgcgccagc gtggcccgct tcccttccgg caggcgtcag
     5521 cctgccaggg gttttcgcgc ggactgacag gcggcctcca ccgcctgccc ctgtatcacc
     5581 tgtttctgct accctgtaag atgtagtgac cgcccaggaa aaccgcctgc tgattcgcca
     5641 caaaatcgca gatttcatca acaatctgcg cggtggtgag cggtttctgc cccatttcac
     5701 gacgccgcgc attaatcacg gccctgtaat tacgcagaat ttcgcgggtt tctgtgctga
     5761 tattcatgac atcgtctccc cgtccagcgc ctgtaacaca acgtccattg cttcgcgttc
     5821 ctccctgacg aatgcgtcac cgtcgccccc gttctcactc ccgacggcat acaggcgctt
     5881 catttcactg cctgccagcg ccatcacttt ttccagcatc accgccggga ctgtcacctg
     5941 acggctggcg cgcttttctt ccatcagttc acgaacaacg ccgtgaacaa cgctgacatc
     6001 cgcaccatat tcaaaggcat cgtccagccg ctgcatgacg gtttccagct catcgtctgt
     6061 cagactccag tcctgggcta tgtgacgcac atccccacgg gtcagcacta cggcaaaaac
     6121 gcattcactg tccggcacgt ctgtatccag cacttcgcgg ataatttctt taacagtaca
     6181 gtacatgttc tcgtctcctt tacaggggcg gcattgccgc cccgtccgtt aatcagtcca
     6241 gagccgcgta aatcgcgttg cattcggggt gaaattcgat gtgacgccac aactgcgcat
     6301 cgcgcatccg gtaaagctgg gtcagtcctg catccccgct gtcgtggtac agccacaact
     6361 ggcggttaat cacaagggag gtcaggatga tgcctgcggc atcagcactc acggtacggt
     6421 caaaccagtt tgcaccgttc accatgtgga aacggtcgcc atccggcatc atgtaaccgc
     6481 caccgtccgg caggcggatg aagtgccaga agcctccctg atattccggc atcatttttt
     6541 gcgccagcgc gtacacatac gcctcggcat acagaaaatc gttgccaaac agatacggca
     6601 gaaaggagag acggtcgcac tcttcaacat tcagagtgac aggtttcgca tattgcatga
     6661 tgttttcctc cgttgttcac atattccgcg acaccgggcg gtgccgtgcc tccataaaag
     6721 ccgggcgaac aggcggggat gcaggggcgc aagggaagcc gcagcctgac gcacacggct
     6781 gacgggagtt gcagggtgag accgcgaaat ctgagcgggt gaacgaccgg aaaatcacgg
     6841 cagacgggaa ggagggaccg ggtgaccgca gcggcgaagc gtacccttgc atcaacgcca
     6901 gcccggctaa gatggaggca cggcacagcc cgcacacgac gctgaggcca cataactgaa
     6961 aaaccacaag gccatgccct gcatggccag gtaaagcgga gccggcggcg acttcctttg
     7021 tgctctgtcc ggggaccggc aggaaaaagg cggggattta ccccgccagt gtcatcattt
     7081 ccggattttt gccagggcat ccgccatgct gcggatttcg gtccgggctt cccggaccac
     7141 ctcccgcaga acaccacggt caaaccagcg gcggggcggt tcatccggta tacgcagcac
     7201 atcgcaggcc agcccactgg acaggatatg ctcaatacac tcactgacca gatacatttc
     7261 caccgacgaa aaacgtgctc cccggttcac gatggcatcg agcaccacgg gcaggttttc
     7321 gtcatcttca tcgagccagc aggggtgatc gctttccttc acgaccaggc gaaagcggcg
     7381 cgtctggcgg gtcagcaacg tctgaccggt ggtggtcagc gccgcacgga tacgccgcgc
     7441 atcttctgcc acctggtaac agatgcttcg taccgcatcg tgcaccgcat caagaacagc
     7501 cctgatttcg tcttcggtca gccattctcc ggcatacggg aaagacacct gcgttccgtc
     7561 tgccggaagt accggcagcg actgccactg actgccggac agtaccagtt ccgccccggt
     7621 tccggtcagt ctctgaatgg cggaggccag gagcggcggc agtacgccgc cgtgacggtt
     7681 gataagtaac gtcgccagcc ggggcgcatc cagccgcacg taaatgctga tccagcccgg
     7741 atagttcgga tttcgctggt caacatccgt ctcttccggc cagccctgcg ccagcagcag
     7801 gttacgggcg tgacgacaaa tcagtgcgtt cagcgtgctg ttttcgttca tgattaagcg
     7861 ttctccctgt atcgccgccc gcagggcagg cggcgataac atcagttgtt ttcgtgtgtg
     7921 ttgtttttca gcagggtgcg ggcctgatta accaccgcct gacggcgggt aacgcattcc
     7981 gggacggcga ggccgttatc acgggcggcc cactcacgtt tccacagtgc ctgttcagcg
     8041 gtctgaatca gggacgccag cagttcagca gggacggtca cctggcggtt ttcgctcacg
     8101 ttgctgataa tctccatcac cgcggcggaa gaaataccgg attcagtccg ctggtcttcc
     8161 gggatgtcct ccatgcgcgc caggacggtc ctgatttcat gatcggtcag agaaatttcc
     8221 ataccgtcag ccagtgcctg aatctcttcc ggggaccaga taaccagcat cagcggcgcg
     8281 tctcccggat actttgctgc cagctcccgg catagcgttt cgcatgttcc gtacattctc
     8341 gtctccttct caatctttga agcaggaatc cagttccaga atttcggtat acgcctccat
     8401 aaacaggcga ttggcgcgat acagcgtctg gcgtgtcagg cgttcaacaa gcacatcgga
     8461 atcaaagcga cagcgatgct ccatatcgtt cagcctggcc cgaaggtcac acatggtgtt
     8521 ggccacctcc gccacgtcgg cgcgaagttt ttcgtgtccg gcatagttca tttacgcagc
     8581 ctccggcata aaccagtcat cattcgcggc cccctgctgc atggcccgtt gcacggcggc
     8641 aaggcgttgc tgcccggcac ggtgatactg ctcaagcagc tcgataccga tataccggcg
     8701 tccggactgg agggcggcga cgcaggttga gccgctgcct gcaaacgggt ccagcacaat
     8761 tgcgttcggg tgtgtgaagc tctcaatcag cggttgcagg ctggtaacgg gcttttccgt
     8821 cgggtgatga cgattgccag aatatttcca gcccagcacg tccggcagcg ggttttgtgg
     8881 cagacgtgga cggccttttg ccaggatgta ggcgcattcg tggcgatagc ccacatatgc
     8941 ggccttcgat gtgtagtttt tggtgaagac caggtgacca acaacgctga atcccgcatt
     9001 tttccaggcg gccataaagc gatcgacgcg gttccagccg tagaagctca ccatcagcgc
     9061 gtcttttttg agtacgcggt acatttcatt acaggccggt tgcagccatt catcggtttt
     9121 atcgccggcg atggtgcgcc cggaacggtc acgaaaaccg acgagatatg gcgggtcagt
     9181 taatatgaaa tcaatggcgt tatcagggat acgcgtcata acatcgatgc agttaccaag
     9241 gacaaaacgg gacataacaa cgctccttct cttacagagg gcaccgggag ggcccccctg
     9301 tggtgcccgt tccccgctgc ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga
     9361 ccgtggaata tcgcagccgt cgtctttttg acggcgagaa tatgccgcga aagcggcttg
     9421 aagggcgttc atcagaaaag ggcgacaggc tggcgtacac tgtcaccggt gccagccggg
     9481 agcagtaaaa gcgcccgtcc cgtgcccacc ggacgcggga cactgaccac aggcagcgtt
     9541 cctgccgcgc cgcgtcagcc ggcgcagtaa aaagaggagc acggcgacat cctttgtcgg
     9601 aaaagaaacg ggcggttgcc cgcccgtctc cgtcatactt catcccccgt aatgcgccac
     9661 cttgtcgact atccactccg gcgcggtgac ttccggcagc tcatcatcat ctgtcgggga
     9721 agaccattcc agttcccccc acagcacatc aacaagctct ccacgctcat atcgctgcca
     9781 gccacagaaa ttgcagccct gttcggcgta ccagtgctcc agcgtgcagc cgaaacggcg
     9841 gcttaattct gcaacaacgt tgctttccgg ctgacaccac ggcgtgtcga aatccacttg
     9901 aatgaagttt tctccctgac ggctgatatt cacctcatac cccacaggcc acttcacgcc
     9961 atactgttcc gtataccagt gatacgcaga aggaacaccg ttcagaacac caccgttaaa
    10021 gccattcact tcaacatcca ggcgggtggg cagaaccgtc agcaggtcaa acggcatggt
    10081 tttttccggc agtacattgt cacacagacg gttccaccac accgatacat cctcgttact
    10141 ccagaagcca caccagtcac ctctttttgc ggtgaaatgc acggtgatgg tttccctgac
    10201 ctcatccggt aatccttccc agcgacgctg gccagtacca ctctgcagcc agagttcatg
    10261 cagcatcagg cagttttgct catccagcag tacaccgttc tgcaggtgtg tcagccagcg
    10321 ggtgaacgcg atattctccg gcgatacaac accacgtccg gcatccgtca gtccggggca
    10381 cggttcaaac tgcacatctt cagtggtctg cagaagtccg gcacttccgg ccagaaacag
    10441 ctgaatacct tcatttgtgg cgcggcgata aagcggtgtg acggcaccgc tggccagtcg
    10501 tttaatctca gcaatctgtg ccggttcacc agaaaaatgc atacgattcg agcaccagtt
    10561 tggcatggga attctcctct cacatgcggc ctccatcagg ccgtgccgca cctgtaaccg
    10621 ggcgaccgca tacgaatgca ggtgcggccc cgaagccgca gcgcgtggag gaggaacgac
    10681 gaaacgtgac cgggtgagga ggccggcgac agcgacacac ttgcatcgga tgcagcccgg
    10741 ttaacgtgcc ggcacggcct gggtaaccag gtattttgtc cacataaccg tgcgcaaaat
    10801 gttgtggata agcaggacac agcagcaatc cacagcaggc atacaaccgc acaccgaggt
    10861 tactccgttc tacaggttac gacgacatgt caatacttgc ccttgacagg cattgatgga
    10921 atcgtagtct cacgctgata gtctgatcga caatacaagt gggaccgtgg tcccagaccg
    10981 ataatcagac cgacaacacg agtgggatcg tggtcccaga ctaataatca gaccgacgat
    11041 acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt
    11101 cccagactaa taatcagacc gacgatacga gtgggaccat ggtcccagac taataatcag
    11161 accgacgata cgagtgggac catggtccca gactaataat cagaccgacg atacgagtgg
    11221 gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagtct
    11281 gattatcaga ccgacgatac aagtggaaca gtgggcccag agagaatatt caggccagtt
    11341 atgctttctg gcctgtaaca aaggacatta agtaaagaca gataaacgta gactaaaacg
    11401 tggtcgcatc agggtgctgg cttttcaagt tccttaagaa tggcctcaat tttctctata
    11461 cactcagttg gaacacgaga cctgtccagg ttaagcacca ttttatcgcc cttatacaat
    11521 actgtcgctc caggagcaaa ctgatgtcgt gagcttaaac tagttcttga tgcagatgac
    11581 gttttaagca cagaagttaa aagagtgata acttcttcag cttcaaatat caccccagct
    11641 tttttctgct catgaaggtt agatgcctgc tgcttaagta attcctcttt atctgtaaag
    11701 gctttttgaa gtgcatcacc tgaccgggca gatagttcac cggggtgaga aaaaagagca
    11761 acaactgatt taggcaattt ggctgtgttg atacagcggg taataatctt acgtgaaata
    11821 ttttccgcat cagccagcgc agaaatattt ccagcaaatt cattctgcaa tcggcttgca
    11881 taacgctgac cacgttcata agcacttgtt gggcgataat cgttacccaa tctggataat
    11941 gcagccatct gctcatcatc cagctcgcca accagaacac gataatcact ttcggtaagt
    12001 gcagcagctt tacgacggcg actcccatcg gcaatttcta tgacaccaga tactcttcga
    12061 ccgaacgccg gtgtctgttg accagtcagt agaaaagaag ggatgagatc atccagtgag
    12121 tcctcagtaa gcagctcctg gtcacgttca ttacctgacc atacccgaga ggtcttctca
    12181 acactatcac cccggagcac ttcaagagta aacttcacat cccgaccaca tacaggcaaa
    12241 gtaatggcat taccgcgagc cattactcct acgcgcgcaa ttaacgaatc caccatcggg
    12301 gcagctggtg tcgataacga agtatctcca accggttgag tattgagcgt atgttttgga
    12361 ataacaggcg cacgcttcat tatctaatct cccagcgtgg tttaatcaga cgatcgaaaa
    12421 tttcattgca gacaggttcc caaatagaaa gagcatttct ccaggcacca gttgaagagc
    12481 gttgatcaat ggcctgttca aaaacagttc tcatccggat ctgaccttta ccaacttcat
    12541 ccgtttcacg tacaacattt tttagaacca tgcttcccca ggcatcccga atttgctcct
    12601 ccatccacgg ggactgagag ccattactat tgctgtattt ggtaagcaaa atacgtacat
    12661 caggctcgaa ccctttaaga tcaacgttct tgagcagatc acgaagcata tcgaaaaact
    12721 gcagtgcgga ggtgtagtca aacaactcag caggcgtggg aacaatcagc acatcagcag
    12781 cacatacgac attaatcgtg ccgataccca ggttaggcgc gctgtcaata actatgacat
    12841 catagtcatg agcaacagtt tcaatggcca gtcggagcat caggtgtgga tcggtgggca
    12901 gtttaccttc atcaaatttg cccattaact cagtttcaat acggtgcaga gccagacagg
    12961 aaggaataat gtcaagccca ggccagcaag tgggctttat tgcataagtg acatcgtcct
    13021 tttccccaag atagaaaggc aggagagtgt cttctgcatg aatatgaaga tctggtaccc
    13081 atccgtgata cattgaggct gttccctggg ggtcgttacc ttccacgagc aaaacacgta
    13141 gccccttcag agccagatcc tgagcaagat gaacagaaac tgaggttttg taaacgccac
    13201 ctttatgggc agcaaccccg atcaccggtg gaaatacgtc ttcagcacgt cgcaatcgcg
    13261 taccaaacac atcacgcata tgattaattt gttcaattgt ataaccaaca cgttgctcaa
    13321 cccgtcctcg aatttccata tccgggtgcg gtagtcgccc tgctttctcg gcatctctga
    13381 tagcctgaga agaaacccca actaaatccg ctgcttcacc tattctccag cgccgggtta
    13441 ttttcctcgc ttccgggctg tcatcattaa actgtgcaat ggcgatagcc ttcgtcattt
    13501 catgaccagc gtttatgcac tggttaagtg tttccatgag tttcattctg aacatccttt
    13561 aatcattgct ttgcgttttt ttattaaatc ttgcaattta ctgcaaagca acaacaaaat
    13621 cgcaaagtca tcaaaaaacc gcaaagttgt ttaaaataag agcaacacta caaaaggaga
    13681 taagaagagc acatacctca gtcacttatt atcactagcg ctcgccgcag ccgtgtaacc
    13741 gagcatagcg agcgaactgg cgaggaagca aagaagaact gttctgtcag atagctctta
    13801 cgctcagcgc aagaagaaat atccaccgtg ggaaaaactc caggtagagg tacacacgcg
    13861 gatagccaat tcagagtaat aaactgtgat aatcaaccct catcaatgat gacgaactaa
    13921 cccccgatat caggtcacat gacgaaggga aagagaagga aatcaactgt gacaaactgc
    13981 cctcaaattt ggcttcctta aaaattacag ttcaaaaagt atgagaaaat ccatgcaggc
    14041 tgaaggaaac agcaaaactg tgacaaatta ccctcagtag gtcagaacaa atgtgacgaa
    14101 ccaccctcaa atctgtgaca gataaccctc agactatcct gtcgtcatgg aagtgatatc
    14161 gcggaaggaa aatacgatat gagtcgtctg gcggcctttc tttttctcaa tgtatgagag
    14221 gcgcattgga gttctgctgt tgatctcatt aacacagacc tgcaggaagc ggcggcggaa
    14281 gtcaggcata cgctggtaac tttgaggcag ctggtaacgc tctatgatcc agtcgatttt
    14341 cagagagacg atgcctgagc catccggctt acgatactga cacagggatt cgtataaacg
    14401 catggcatac ggattggtga tttcttttgt ttcactaagc cgaaactgcg taaaccggtt
    14461 ctgtaacccg ataaagaagg gaatgagata tgggttgata tgtacactgt aaagccctct
    14521 ggatggactg tgcgcacgtt tgataaacca gggaaaagat tcatagcctt tttcatcgcc
    14581 ggcatcctct tcagggcgat aaaaaaccac ttccttcccc gcgaaactct tcaatgcctg
    14641 ccgtatatcc ttactggctt ccgcagaggt caatccgaat atttcagcat atttagcaac
    14701 atggatctcg cagataccgt catgttcctg tagggtgcca tcagattttc tgatctggtc
    14761 aacgaacaga tacagcatac gtttttgatc ccgggagaga ctatatgccg cctcagtgag
    14821 gtcgtttgac tggacgattc gcgggctatt tttacgtttc ttgtgattga taaccgctgt
    14881 ttccgccatg acagatccat gtgaagtgtg acaagttttt agattgtcac actaaataaa
    14941 aaagagtcaa taagcaggga taactttgtg aaaaaacagc ttcttctgag ggcaatttgt
    15001 cacagggtta agggcaattt gtcacagaca ggactgtcat ttgagggtga tttgtcacac
    15061 tgaaagggca atttgtcaca acaccttctc tagaaccagc atggataaag gcctacaagg
    15121 cgctctaaaa aagaagatct aaaaactata aaaaaaataa ttataaaaat atccccgtgg
    15181 ataagtggat aaccccaagg gaagtttttt caggcatcgt gtgtaagcag aatatataag
    15241 tgctgttccc tggtgcttcc tcgctcactc gagggcttcg ccctgtcgct cgactgcggc
    15301 gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat taggttgttc
    15361 tgtccattgc tgacataatc cgctccactt caacgtaaca ccacacgaag atttctattg
    15421 ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg acagaacact
    15481 acttcctata aacgctacac aggctcctga gattaataat gcggatctct acgataatgg
    15541 gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt ctctgtttaa
    15601 cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca aggcatttat
    15661 tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc aaactgcacc
    15721 cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt tgaactgatg
    15781 gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata cagcatgtgc
    15841 atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt cacaccgtca
    15901 gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc cgtcacttcc
    15961 cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat gggtattttc
    16021 agtgttgcca ccatcgtctg cagctggctg acgtaccagg agtcagagag cggaaccagc
    16081 cggtgagtct gctgaccggc gggcattctc cccgccgtcc tggcagcttt ttcggtccgt
    16141 tgtttcaggg tcgcaagctg cacaaacgga tacggaggcg caagcgaaaa atccccccgc
    16201 gtcagcgcca gtgcttcatt aatgcgtgct ccggtgttcc acagtgtggc cagcagcatc
    16261 ttgcggtgca gatccgggac gtaatggagc agggcactca cttccggagc cagcagatat
    16321 tttggcagtt catcatggac catcgacatc tggcgaagtg ccagagctgc cggataatca
    16381 atggcaacag gcagcgatgc aggctgcccg gcagaataca ctgccggtac catgactgca
    16441 gactggctgt gtataacgga gcctgacatt tatattcccc agaacatcag gttaatggcg
    16501 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
    16561 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
    16621 gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc
    16681 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
    16741 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagtccctgt tctcgtcagc
    16801 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
    16861 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc
    16921 ggagatattg acatcatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
    16981 aatacgctgc ttcatagcac acctcttttt gacatacttc gggtatacat atcagtatat
    17041 attcttatgc cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
    17101 ccttatgtat tttacctttc gttatgttaa ccaataaaaa ttaaaatctg acttataaaa
    17161 acaaagcgta attaccgcat tcccgtttcg tatggatccg actttatttg aataaacgac
    17221 tttccggaag tgtacaacgc tgagaaaatc atcatatcgc tgttttccgc ccctgcgcgg
    17281 cgcatcacag tccagagata caacacaaca tatagtgcca ttggcaactg tatgaggcac
    17341 tacatgctgt gctgacggga tgtaacgctg tgcggaggga gaggaagatc cgtaacttac
    17401 ttttttcgga tctctggaga ggggaagaag gatttgtgct gatgatgcaa tactaatgat
    17461 atattttgaa cttaattgca ttattgctta tctggcatta ataactgaaa tactttgcat
    17521 aattatcaat gaaagaacga ggctcatatt tcccatgtaa attatatgcc cttgcagaga
    17581 gcgagacggt agtgaacttg cggcactaat aataaactgt accgactctt cccgggcact
    17641 ggaatgttca gatgtacgac agagccccgc gccttttgca ataaataatt aaaggactga
    17701 acatggcgac cacagtaata gctgccttta acgaatttat gaaagatacc gtgaatctca
    17761 aaaaggcaga taccgatgac gcgcgtgcaa gtcgcgactg gcttatcggt aagatgaatg
    17821 attttgagaa ggacgataaa tttccggtga gttttccagc gatccatatt gcctttggct
    17881 cctttgccag aagaaccaaa atccgtccgc ttgatgatat cgatctgatg tttggcttaa
    17941 ccgggcaagg tgccacctat accatcctga gtgaccggat aactgtaacc tcctccggag
    18001 aggggtcacg tctgcacagt taccgccatt ccggagctga caccgtctgc tccgtcagaa
    18061 ttctgaatgc ctttaaaaac cgcctgcagg acattgccca gtacgcccag gctgatatca
    18121 ggcgtaacca ggaagctgtc acgctgaaac tggtcagcaa ggactggaat tttgacatcg
    18181 tcccctgctt tattaccagc gaagatgcct ttggacggac ttactacctg atccccgatg
    18241 gcaacggcca ctggaagttt accgaccccc gtaaagacag ggacagagtc actactatca
    18301 atgtgcagaa taacggcaat gtgctcaatg tcatcagagc agtcaaatac tggcagcggc
    18361 gaccgacgat gccatccatg agttcctacc tgctggaaac gctgattctg gactattacg
    18421 ctggcaggac atcctgctca tcgtttgttg acatggagct ggaagcgctt ttccgccacc
    18481 ttggtcaatc cgtgcggtac tctgtcaacg atcccaaagg catacagggt gatatcaact
    18541 cactttcagc cgaggcacgt aaggcgatat cggacagatg ttatctcgat gcgcaaaagg
    18601 tatcggaggc gagatggttt gaaaataata aagagtatga aaaatccatc aataaatggc
    18661 gcgatgtgtt cgggccattc tttcctgttt acgggtgaat catgaatgcc atttacacaa
    18721 accagtcgac cagagaaaac atggatttac tgtatgcgca ggcccgtgtc tacgacaggg
    18781 taaaaaactg gaacagactg aattttctct tcagcatcat cgtaccgctc ctgctttctt
    18841 tcgttaccgt atacaaccgg agccgggaat ttgtcgattc agagttgttg tcttcgctgt
    18901 tggggttata tggactcctg gtgcttacct ttaacattgt tatcagtgga cacatctcgg
    18961 ctctgcgcag gaaggcagcc tcaatccagg aaatgtacga ctgccgggta cttggcatcc
    19021 ggcgtaatga actgaaggtg gaggagatcc cacgggatga catcatccgg gaagctgcat
    19081 attttagaga cagcccggaa aaagcacgaa agcgatttgg cgaagagggg tggtacgtca
    19141 gtaaggtgta tgacgcgccg caggccgtaa tggcgctcct ttgccacgga aaaaatctcg
    19201 gctgggacaa atcgctcagg gaggttctgc acgtatttta tctttccgct tttattgtct
    19261 cgcctgtcgc gatgcttgtt tacggcatcg tcatgaaatc cgggctgaat gagatcctct
    19321 tctatgtggt gttcacactg ccggtcatcc gctatttctt gcttcagttt ctggataatc
    19381 gcagcagtat gaaaagaagc gagaagctga aaaagtacgt tgagaaggaa ttatccggca
    19441 taagggtgtc cggaagggca gaagaagagc aactggctta tacgctccgg aatatccaag
    19501 atgagatgtt tacctaccgg gcctcatgtc cgcccgtacc caacggtatt cagctgataa
    19561 tgaagcccaa aaatgaacga atttatgttg attactttga aacaaatctg aaggagctgc
    19621 accttcagaa gtaatccctc cttagctccg gtgtaggatt tacgtatgcc ggagcacatc
    19681 cgataaacaa ttcttggttg cctgagtgtc agcgtaaacc catagtcacc ttcgcctcag
    19741 acggttagta acacaggtaa tgactccaac ttactgatag tgttttatgt tcagataatg
    19801 cccgatgact ttgtcatgca gctccaccga ttttgagaac gacagcgact tccgtcccag
    19861 ccgtg
//
LOCUS       DAYPHN010000082        19775 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000082 DAYPHN010000000
VERSION     DAYPHN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.082"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(212..355)
                     /gene="ypaB"
                     /locus_tag="ACG0BW_002805"
     CDS             complement(212..355)
                     /gene="ypaB"
                     /locus_tag="ACG0BW_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFK7259745.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            483..2768
                     /gene="nrdA"
                     /locus_tag="ACG0BW_002806"
     CDS             483..2768
                     /gene="nrdA"
                     /locus_tag="ACG0BW_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFK7259746.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3002..4132
                     /gene="nrdB"
                     /locus_tag="ACG0BW_002807"
     CDS             3002..4132
                     /gene="nrdB"
                     /locus_tag="ACG0BW_002807"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFK7259747.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4132..4386
                     /gene="yfaE"
                     /locus_tag="ACG0BW_002808"
     CDS             4132..4386
                     /gene="yfaE"
                     /locus_tag="ACG0BW_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFK7259748.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4440..5090)
                     /gene="inaA"
                     /locus_tag="ACG0BW_002809"
     CDS             complement(4440..5090)
                     /gene="inaA"
                     /locus_tag="ACG0BW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFK7259749.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5171..6361)
                     /locus_tag="ACG0BW_002810"
     CDS             complement(5171..6361)
                     /locus_tag="ACG0BW_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259750.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLLVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLVFFTVGLVVLGYTQGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6513..7391
                     /locus_tag="ACG0BW_002811"
     CDS             6513..7391
                     /locus_tag="ACG0BW_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7259751.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVHHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPAVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
     gene            7857..8927
                     /locus_tag="ACG0BW_002812"
                     /pseudo
     CDS             7857..8927
                     /locus_tag="ACG0BW_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9148..10224)
                     /gene="glpQ"
                     /locus_tag="ACG0BW_002813"
     CDS             complement(9148..10224)
                     /gene="glpQ"
                     /locus_tag="ACG0BW_002813"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFK7259752.1"
                     /translation="MKLTLKNLSMAIMMSTMVMGSGAMAADNNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10229..11587)
                     /gene="glpT"
                     /locus_tag="ACG0BW_002814"
     CDS             complement(10229..11587)
                     /gene="glpT"
                     /locus_tag="ACG0BW_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFK7259753.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPNVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            11860..13488
                     /gene="glpA"
                     /locus_tag="ACG0BW_002815"
     CDS             11860..13488
                     /gene="glpA"
                     /locus_tag="ACG0BW_002815"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFK7259754.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDSESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13478..14737
                     /gene="glpB"
                     /locus_tag="ACG0BW_002816"
     CDS             13478..14737
                     /gene="glpB"
                     /locus_tag="ACG0BW_002816"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFK7259755.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCTIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSVLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            14734..15924
                     /gene="glpC"
                     /locus_tag="ACG0BW_002817"
     CDS             14734..15924
                     /gene="glpC"
                     /locus_tag="ACG0BW_002817"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFK7259756.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYHSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            16117..17076
                     /gene="rpnE"
                     /locus_tag="ACG0BW_002818"
     CDS             16117..17076
                     /gene="rpnE"
                     /locus_tag="ACG0BW_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFK7259757.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNGH"
     gene            17077..17286
                     /locus_tag="ACG0BW_002819"
                     /pseudo
     CDS             17077..17286
                     /locus_tag="ACG0BW_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17327..18130)
                     /gene="yfaU"
                     /locus_tag="ACG0BW_002820"
     CDS             complement(17327..18130)
                     /gene="yfaU"
                     /locus_tag="ACG0BW_002820"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFK7259758.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(18147..19421)
                     /gene="yfaV"
                     /locus_tag="ACG0BW_002821"
     CDS             complement(18147..19421)
                     /gene="yfaV"
                     /locus_tag="ACG0BW_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259759.1"
                     /translation="MNVAGIHVLQGVEDVKLALYVLAFLDRSNIGFAKQTYQIDTGLS
                     NEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWADTEAKF
                     LIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLSGALLE
                     MHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQLASEE
                     QQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFTASVVT
                     AIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVALCVAA
                     IGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFASDAAG
                     LLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(19418..>19775)
                     /locus_tag="ACG0BW_002822"
     CDS             complement(19418..>19775)
                     /locus_tag="ACG0BW_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFK7259760.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQLLAVPPA
                     YTSQRCACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         5266 a         5194 c         4705 g         4610 t
ORIGIN      
        1 ataaggtaaa ttaacaaaat ggcttcgcat ttaacaataa ccgaagagaa aacaaccatt
       61 tcgatatcaa caatcgctta cattcgctta tatattgacc agaactgata catcagatta
      121 tgtgatgact cgtgcttaga tcaatttttg caatcattag ccaaaagatt aataaaccat
      181 ctatatcaat ttatctaacc tattcttccg ttcaagaaat cgccgaacag ttatttttaa
      241 caaatttttc tcttcccatt gactttcccg gacaccttgt ctgacctaag gtgcgcgaaa
      301 gccacttttt ccttcctgag ttatccacaa agttatgcac ttgcaagagg gccattttca
      361 cactatcttg cagtgaatcc caaacatacc ccctatatat agtgttctaa gcagcttccc
      421 tcactacagg tagtctgcat gaaactattg cggaaagaat tccaaaaaca ggtacgacat
      481 acatgaatca gaatctgctg gtgacaaagc gcgacggtag cacagagcgc atcaatctcg
      541 acaaaatcca tcgcgttctg gattgggcgg cagaaggact gcataacgtt tcgatttccc
      601 aggtcgaact gcgctcccac attcagtttt atgacggtat caagacctct gacatccacg
      661 aaaccattat caaggctgcc gcagacctga tctcccgtga tgcgccggat tatcagtatc
      721 tcgccgcgcg cctggcaatc ttccacctgc gtaaaaaagc ctacggccag tttgagccgc
      781 ctgcgctgta cgaccacgtg gtgaaaatgg tcgagatggg caaatacgat aatcatctgc
      841 tggaagacta cacggaagaa gagttcaagc agatggacac gtttatcgat cacgaccgtg
      901 atatgacctt ctcttatgct gccgttaagc agctggaagg caaatatctg gtacagaacc
      961 gcgtgaccgg cgaaatctac gaaagcgccc agttccttta tattctggtt gccgcgtgcc
     1021 tgttctcgaa ctacccgcgt gaaacgcgcc tgcaatacgt gaagcgtttt tacgacgcgg
     1081 tttccacatt taaaatttcg ctgccgacgc caatcatgtc cggcgtgcgt accccgactc
     1141 gtcagttcag ctcctgcgta ctgatcgagt gcggcgacag cctggattcc atcaacgcca
     1201 cctccagcgc gattgtgaaa tatgtttccc agcgtgccgg gatcggcatc aatgccgggc
     1261 gtattcgtgc gctgggtagc ccgattcgcg gtggcgaagc gttccatacc ggttgtattc
     1321 cgttctataa gcatttccag acagcggtga aatcctgctc tcagggcggt gtgcgtggcg
     1381 gtgcggctac gctgttctac ccgatgtggc atctggaagt ggaaagcctg ttggtgttga
     1441 aaaacaaccg tggggtggaa ggcaaccgcg tgcgtcatat ggactacggg gtacaaatca
     1501 acaaactgat gtatacccgt ctgctgaaag gtgaagatat caccctgttc agcccgtccg
     1561 acgtaccggg gctgtacgac gcgttcttcg ccgatcagga agagtttgaa cgcctgtaca
     1621 ccaaatatga gaaagacgac agcatccgca agcagcgtgt gaaagccgtt gagctgttct
     1681 cgctgatgat gcaggaacgt gcgtctaccg gtcgaatcta tattcagaac gttgaccact
     1741 gcaataccca tagcccgttt gatccggcca tcgcgccagt gcgccagtct aacctgtgcc
     1801 tggaaatcgc cctgccgacc aaaccgctga atgatgtcaa cgacgagaac ggtgaaatcg
     1861 cgctgtgtac gttgtctgct ttcaacctgg gcgcaattaa tagcctggat gaactggaag
     1921 agctggcgat tctggcagta cgcgcactcg acgcgctgct ggattatcag gattacccga
     1981 tcccggccgc caaacgtgga gcgatgggtc gtcgtacgct gggtattggt gtgatcaact
     2041 tcgcttacta cctggcgaag cacggtaaac gctactccga cggcagcgcc aacaacctga
     2101 cacataaaac cttcgaagct attcagtatt acctgctgaa agcctctaat gagctggcga
     2161 aagagcaagg cgcgtgcccg tggtttaacg aaaccactta cgcgaaaggg atcctgccga
     2221 tcgataccta taagaaagat ctggatgcca tcgctaatga gccgctgcat tacgactggg
     2281 aagcactgcg tgagtcaatc aaaacgcacg gcctgcgtaa ctccacgctt tctgctctga
     2341 tgccgtcaga gacttcttcg cagatctcta acgccactaa cggtattgaa ccgccgcgcg
     2401 gttacgtcag catcaaagcg tcgaaagacg gtattctccg ccaggtggtg ccggactacg
     2461 agcatctgca cgacgcctat gagctgctgt gggaaatgcc gggtaacgat ggctacctgc
     2521 aactggtggg tatcatgcag aaatttatcg atcagtcgat ctctgctaac accaactacg
     2581 atccgtcacg cttcccgtca ggaaaagtgc cgatgcagca actgctgaaa gacctgctca
     2641 ccgcctacaa attcggggtc aaaacgctgt attatcagaa cacccgtgac ggcgctgaag
     2701 acgcacaaga cgatctggtg ccgtcaatcc aggacgatgg ctgcgaaagc ggcgcatgta
     2761 agatctgata ttgagatgcc ggatgcggcg tgaacgcctt atccggccta cggctcggtt
     2821 tgtaggcctg ataagacgcg ccagcgtcgc atcaggctcc gggtgccgga tgcggcgtaa
     2881 acgccttatc cggcctacgg ctcggatttg taggcctgat aagacgcgcc agcgtcgcat
     2941 caggcacagg atgcggcgta aaatgcctta tccggcatta aactcccaac aggacacatt
     3001 catggcatat accacctttt cacagacgaa aaatgatcag ctgaaagaac cgatgttctt
     3061 tggtcagccg gttaacgtgg ctcgctacga tcagcaaaaa tatgacatct tcgaaaagct
     3121 gatcgaaaag cagctctctt tcttctggcg tccggaagaa gttgacgttt ctcgtgaccg
     3181 tatcgattac caggcactgc ctgaacacga aaaacacatc tttatcagca acctgaaata
     3241 tcagacgctg ctggactcca ttcagggtcg cagcccgaac gtggcactgc tgccgctgat
     3301 ctcgatccct gaactggaaa cctgggtcga aacctgggcg ttctcagaaa ccattcactc
     3361 tcgttcttat acccatatca ttcgtaatat cgttaacgat ccgtccgttg tgtttgatga
     3421 tatcgtcacc aacgagcaga tccagaaacg tgcggaaggg atctccagct attacgatga
     3481 gctgatcgaa atgaccagct actggcatct gctgggcgaa ggtacccaca ccgttaacgg
     3541 taaaaccgta accgttagcc tgcgcgagct gaagaaaaaa ctgtatctct gcctgatgag
     3601 cgttaacgcg ctggaagcga tccgcttcta cgtcagcttt gcttgttcct ttgcctttgc
     3661 agaacgcgaa ctgatggaag gtaacgccaa aattattcgt ctgattgccc gcgacgaagc
     3721 cctgcacctg accggtactc agcatatgct gaatctgctg cgcagcggtg cggatgatcc
     3781 ggagatggcg gaaattgccg aagaatgtaa gcaggaatgc tatgacctgt ttgtccaggc
     3841 ggcacagcaa gagaaagact gggcggatta tctgttccgt gacggttcga tgattggtct
     3901 gaataaagac attctctgcc agtacgttga atacatcacc aatatccgca tgcaggcagt
     3961 cggtttggat ctgccgttcc agacacgctc caacccgatc ccgtggatca atacctggct
     4021 ggtgtctgat aacgtgcagg ttgctccgca ggaagtggaa gtcagttctt atctggtcgg
     4081 gcagattgac tcggaagtgg acaccgacga tttgagtaac ttccagctct gatggctcgc
     4141 gttaccctgc gcatcactgg cacacaactg ctgtgccagg atgaacaccc ttcccttctg
     4201 gcggcgctgg aatcccacaa tgtggcggtt gagtaccagt gccgcgaagg ttactgcggt
     4261 tcctgtcgca cgcgccttgt tgcaggccag gtggactgga ttgccgaacc gttagccttt
     4321 attcagccgg gggaaatttt gccctgttgt tgccgggcaa aaggcgatat tgaaatcgag
     4381 ttgtgatcgg gtactagcgc cagatacgac gcttacgcgt attatctggc ctacaacgat
     4441 tacatggcgt agtaatacgc tttcacctgc tcccagtccg ctttagggat cggctccaga
     4501 tatttttcca gctggcggaa gtcatgatta atcgctttat cgcgacgcaa gcggcgacga
     4561 cttttctcca gatcaagaaa accagcttct gccttacctt ctgttttcac atagatatgg
     4621 cgaacataac aacagccatg ctgacgatta acgctatgca ttttcttaaa cgccagcgcc
     4681 actgctttca acatggcttg ccgtacttcg tcagaatagg gcgttactgc atgctgggca
     4741 taccagtcag caatgctgat gaaccccgcc atatcttcag tcaccagcaa cgcgcgccat
     4801 tccccctcaa ttttcaccgc ttcaccaaaa acgatcttcg gcacgatgac accggcccgt
     4861 tccagttctt taattaccgc aacctcacgg acaatcgttg gtcggccgaa aggataacgt
     4921 acggaatgaa acagatgatg cgtcatgcgc tttacatata gctttttgcc gttgcgctcg
     4981 acgcattgca ccccgctcat accattacgg cgatagttag gctcttcaac ccagtcgccc
     5041 tctgttgccc accagtgatt aaattcgtcg tgctttgctg aaactgccat accctatcgc
     5101 ctgtcatttt ttattaacga caatgactat aggtggttac ctgcgaaaaa tcttaatgaa
     5161 acgtgtcgta ttaatgaatt tcttttgcca ctactagcgc gacaccgctt ttacccttca
     5221 tttgcagcgc atagactcca ccagccacta ccactaatag tgcacccagc agataaatca
     5281 ttcggtagcc agtatgtgca accatcatcc ccatcacaca ggctcccagc gccaggcctg
     5341 catccatcgc attgaagaat agggagtttg cgacaccgat tttatgcggt tccactgaac
     5401 tgataatctg cgtctgaaaa actggcgtca cggaaccata cccgatacca atcaacgcgc
     5461 cagaaacaac catcattatg cttccctggg tgtagccgag aaccaccagt ccaaccgtaa
     5521 agaacacaag gcaagggtaa accacatatt tcggtccttt tttgtcgcag acattgccgg
     5581 taaaggtacg gcagatcatc aggaagatag cgtagcaaag caggaaatta ctggctgccg
     5641 acatcaggtt aagttcgcgt gcataaagcg ccagaaaagc agaaaccccg gcatacgaaa
     5701 aggtcataaa aaaggtgacc atcgcaaatg gcaacgccgc gcgatcgaac atggcggcga
     5761 agcccatttt cggctttgaa ccatcagcat ggctgataac cggcggcact gtcataatca
     5821 gcgacagaat aatccccacc gctgccacgc cggtgcataa ccagaaagcc atattaaagg
     5881 cctcccagcg cgccatattc aggccaatcc acggcccgac caccattgcc agccccattg
     5941 ccagagagaa aaaactgatg ccctccccac gtcgggaggc cgggatcagc cgcgcggaaa
     6001 tcgttccttt taccgtagta ataaccccga aggtaatacc atgaagcacc cgaataaaaa
     6061 gcagtgattc aattgagtgg caaaaaggat acaacgcagt gaccaacaaa aaggccagag
     6121 aagagagcac cagaatagtt ttattcgaat atttacccac ccattgcccg gcaaaggggc
     6181 gaataacaat ggcggcaatt aaaaataaag tcaccaataa accggcttta tcaggagagg
     6241 catgcaagcg gtcagagata taaattggca acaatgttag taaaacataa aagacgaaga
     6301 aaataataaa gctgatgatg gttattgccc aaaaatcctt cgtccataac ttctctttca
     6361 taccctaccg cctgtcagga tgacgcacgc agacaacact cccgttactg cggttgcgtc
     6421 aaattgtgat gtactttgca gaacagaagt ataaatggcg gcatgctatt agagaaatta
     6481 atcgttttaa tattattgat tagcaaactc taatgactct tacccaaata aattctttac
     6541 tcgccgtgct ggattacggt ggctttactg aagccagcaa gcgattgttc atgacgcagt
     6601 cggcggtaag tcaggcaatt gctgccctcg aacaagaact tggtgtgaat atactgttac
     6661 gcgatcgccg taaagctata caactgacca cagcaggtca tcgcattgtg catcatctgc
     6721 gcgccattaa tcgggaagtt aacgcagtaa aagagatagc cgagcaggaa aagcagaacc
     6781 ctcaaagaac gctgagactt ggttgtttcc ccagcgtctg cgcctgtatc ttaccggctg
     6841 tgattcgcta ctttgaaatt catcatccga atataaaaat tattcctttt gaggaaaata
     6901 gcacagcgat tattgattcc cttcagaatg aatccattga tgccggtttt gttcattttc
     6961 cggtgagcgg aatgtactcc gtgcccattt atcaggataa attcaccgta gtgttaccgc
     7021 ccaaccatgc tctggcaaaa aacagcacta ttacagtgga agagttaatg ggtgaaccat
     7081 taattattag taaaggacgc tatgaattaa gcattatggc actatttaaa gaaaagaata
     7141 ttaccccaca gataaaatat gaatttaatc acccggatac agcgatcagt tttattcgac
     7201 agggattagg catcgcctta ttaccagagt taacgctgaa aaccattgca gatgaattgt
     7261 gttccgttcc tctcgaacca accttctatc gacagatttc gttgctggct aaagaaaagc
     7321 cggtagaagg cagtccactg tttttactac aaatgtgtac ggaacaactc gtggtgagtg
     7381 gaaaaatttg atgtaagaca tgagattgat gacaaatgca agactgcctg attcgccaca
     7441 cttagttgta ctatagcctt taccaggcgt aataattcgt tttcacctga ttttggccca
     7501 ctttgtgaat tggcgatata tatttatcca gttgggagaa gtgacaatga ctccaggtag
     7561 aaaccggtat gaagcgcccg actcactgtc aaaaccgatt gttgagagta atgacactcc
     7621 ggcgaacagt acacagttta ctctggagcc attctcaatc ttcaagtggg atacctaata
     7681 acttgtcagg gaagttatca atatctgttt gttacttatt accgtattta ttaacaataa
     7741 tgatactttc taacatgcgt aaaggtcttc gtaataaaat gagctaaatt tgatagtgaa
     7801 tatattttgc aaagattaga ttgtatcttg ttaactcaca ctacaaggaa tcatttatgc
     7861 caaatacatc tattcactta tctcgctgta atattttaca aaataataaa ttgcaaccag
     7921 cagaagttta caaagaatca caacaaaccg cgaaacttga gattttttgc gatgagtttc
     7981 taaaaatatc tcaaagtaga tacgggttat caacctcagc agacagcata gctaatttat
     8041 tggctttttt cacgaaagcc tcagatgcaa ttgatagtat caagacccaa aaaattgacg
     8101 ttcacagtta tggtttcgta ccaataagac attttgtcga acatgtcata acgtataaaa
     8161 atgaatttgc agcggattat gaaccctata cccttacctt tacccgaggt gaaaacaacg
     8221 aaggtatttt gagtgtcgaa agcaaagaag ggtttatttc acaaagaaca ataaacttaa
     8281 atgaatatga aactgcaatt aacatcatca atgaacatgt tataaaagaa aatatccaca
     8341 acacggtcca gtcaccgaca gaaaaagaca tttcaaaaat aaacagctct gataaacctt
     8401 ataaaaccag cagtgaggaa agtattaaaa gtcaacttta cagcgatcaa aaaaaatata
     8461 cagacctgct actacattct gagaaaaata cagaatggta caaatacgca tcctccgtgg
     8521 aacgctatga taaattcaag aattcttcta aggaaattaa aaatacctac aagcaaattg
     8581 tacaagcgca gaaaaaattg aaccaaatga aatacataaa taaattaagt ggtgagctta
     8641 tcgatatcgc cgacaaaaag ttagcaccat taataaatga tagtttcagc tatacacgag
     8701 atttctttgc ttactcaaaa caggaaaata acatcttcac gtttgataat tctaagtttg
     8761 aagatccaaa ggaaaaaaaa ggtttaatgg tacaacacag caatggacaa ctggttatta
     8821 ctggtgaata ttgtcctgaa ggtgtgcaaa cagcattcac tcaagaccaa tatgataaat
     8881 taataagata cattaatatt ttctttactt ttccgaaatg cgaataatac tttacagtat
     8941 aaactgcaat acaatttatt attgaaccca attcatacta tttaaggaat aacaatgccc
     9001 agtgttagct ttcttaacga atgagtttta cgcaaatatg ttggcatctt ttttaacagg
     9061 atgcaccggt taatgaatag aacaaccggg agtaacatta tttctcttta gcagaggatg
     9121 gcgcatgaac gccatcctgc aatagcatta ctctttatta aggaatttta ctgccttatc
     9181 agggaaatca gtaaacagcc catttacacc cgctttgtta tacagagcat catataactg
     9241 attcacatca gtagtgtatt caggcagttt atctgaccgc acggtataag gatgcactac
     9301 cagcttgttc tgctgagcat cttgcaccat gccagtgagt ttgatattac ccggctgcga
     9361 tgtctcctca atcaacatat ggtaatccgg accaatacca tctgcatatt ccgccacctg
     9421 tttcatggct cccggcttaa acatccagtc gtagttgtaa ttaacccagc ttccgtccgg
     9481 ctgtttctgc tgagtttcgt tccagtcggt ataggcaatc agctgtacca gattgaggtc
     9541 catgcccatt ttaggttcca gctcattctt aatacgcttc agttcatcgg catcaaaaca
     9601 ttgcaaataa actttgtcgt ccttaccggt gtaaccatat ttcttcagca cttccagcgt
     9661 ttttgccgca atatccttcc cttcctgatg atggaaccac ggcgctttaa tttctggata
     9721 gataccgata tttttcccgg tagagtgatt taacccctga acaaattcaa tctcttcttc
     9781 gaaagtgtgt acccggaagt cagatttacc catcgggaaa cggcccggat aagtctgtac
     9841 tttcttaccg ttttcaatat cgaaaccttc ggtaaatttc agtgacttaa tttcatcgag
     9901 cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc cgatccggga aacgatcggc
     9961 aacatcagta acacgatcga gataatggtc atgcaggaca accagatggt cgtctttggt
    10021 catcaccaaa tcttgttcca gataatccgc tccctgcgca tacgccatcg cttttgctgg
    10081 cagcgtatgc tccggcaaat atccactggc accgcgatgg gcgatgacta ttttttcgtt
    10141 gttatccgcc gccattgcac cgcttcccat gaccatagtg ctcatcatga tcgccatgct
    10201 aaggtttttc agcgtcaatt tcatgccatt agcctccgtt gcgtttttgc agtaattcct
    10261 gatggcgacg tttttcgcca atcatcacaa caatcaacaa gataaccgcc agaatgctgc
    10321 cgccaatcat taccataaag ccgccatccc agccgaagaa gtccacggtg tagccaacaa
    10381 tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag cccggtaaag cccgctgccg
    10441 tacctgccgc ttttttcggt gccagttcca gcgcatgcag accgatcaac atcacaggac
    10501 cgtaaatcag gaagccgata acaatcatac agatcatatc gacgttcgga ttgcccgccg
    10561 ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt cataaagaag acgccggtgg
    10621 ccccacggtt accgcggaag actttgtccg acatccagcc gcacagcagg gtgcccggaa
    10681 tgcccgcata ctcatagaag aagtaggccc aggaggattt atccagcgcg aagtgtttca
    10741 cctctttcag ataagtcggt gaccagtcga ggatgccgta acgcagcaga taaacgaaca
    10801 cgttggcgat agcgatgtac cacagcagtt tgttcggcag cacgtactgc atgaagattt
    10861 gcttcgcagt cagctcctgt tccgcttttt cgttatagtc gtccggataa tcatttttgt
    10921 actcttcgat cggcggcagg ccacaggatt gcggggtatc gcgcatcatc gcaaaggcga
    10981 acagtgccac cagaatggcg cagaatgcgg gcatatagag cgcggcgtgc cagtcattga
    11041 accaggccat ccccagcagg aacagcagcg gcggaatacc accaccgacg ttgtgcgcac
    11101 agttccatac tgacacaatg ccgccacgtt ctttctgcga ccaccagtgc accatagtac
    11161 gaccgcacgg cggccatccc atcccctgga accaaccgca gaggaacagc agcacaaaca
    11221 tcaccgcaat gctcgacgtc gcccagggca caaagcccat aaaaagcatc accgccgccg
    11281 ccagaatcaa acctgcgggc aggaaaacgc gcggattcga gcgatccgac accgagccca
    11341 tgatgaattt cgaaaatcca taagcaatcg agatccccga aagggcaaaa cctaaatcac
    11401 cgcgtgagaa gccctgctcg accagataag gcatagcgag cgcaaagttc ttacgaacca
    11461 aatagtaagc agcatagcca aagaatatcc ccaggaaaat ttgccagcgc aatcgacgat
    11521 aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg tggcgctggt ttaaaaatac
    11581 tcaacattga tagcctccgt ggcccgtggt cttatttatg attaacagcc tgattcagtg
    11641 ggagaacctg ccgtttcttg agttgccgcg atgttaagaa aacattcata aattaaatgt
    11701 gaattaccgc acacattatt aaatacgatg ggttaaatgt tcagaatgac gcatgaaatc
    11761 acgtttcact ttcgaattat gagcgaatat gcgcgaaatc aaacaattca tgtttttact
    11821 atggctaaat ggtaaaaaac gaacttcaga gggataacaa tgaaaactcg cgactcgcaa
    11881 tcaagtgacg tgattatcat tggcggcggc gcaacgggag ccgggattgc ccgcgactgt
    11941 gccctgcgcg ggctgcgcgt gattttggta gaacgccacg acatcgcaac cggtgccacc
    12001 gggcgtaacc acggcctgct gcacagcggt gcgcgctatg cggtaaccga ttcggaatcg
    12061 gcccgcgaat gtattagtga aaaccagatc ctgaaacgca ttgcgcgtca ctgcgttgaa
    12121 ccgaccaacg gcctgtttat caccctgccg gaagatgatc tctccttcca ggccactttt
    12181 atccgcgcct gcgaagaagc agggatcagc gcagaagcta tagacccgca gcaagcgcgc
    12241 attatcgaac ctgccgttaa cccggcactg attggcgcgg tgaaagttcc ggatggcacc
    12301 gttgatccat ttcgtctgac cgcagcaaac atgctggatg ccaaagaaca cggtgccgtt
    12361 atccttaccg ctcatgaggt cacgggactg attcgtgaag gcgcgacggt gtgcggcgtt
    12421 cgtgtacgta accatctcac cggcgaaact caggcccttc atgcgcctgt cgtggttaat
    12481 gccgctggga tctgggggca acacattgcc gaatatgccg atctccgcat tcgcatgttc
    12541 ccggcgaaag gatcgctgct gatcatggac caccgcatta atcagcatgt gatcaaccgc
    12601 tgccgcaaac cgtccgacgc cgatattctg gtgcctggcg ataccatttc gctgattggc
    12661 accacctctt tacgtattga ttacaacgag attgacgata atcgagtgac ggcagaagaa
    12721 gttgatattc tgctgcgtga aggggaaaaa ctggcccccg tgatggcgaa aacgcgcatt
    12781 ttgcgggcct actctggcgt gcgtccactg gttgccagcg atgacgaccc gagcggacgt
    12841 aacgtcagcc gtggcatcgt gctgctcgat catgctgaac gcgatggtct ggacggattt
    12901 atcaccatca ccggtggcaa actgatgacc tatcggctga tggcggaatg ggctaccgac
    12961 gcggtatgtc gcaagctggg caatacgcgc ccctgtacca ctgccgacac gccgttgcct
    13021 ggctcacagg aaccggcaga agtcacgctg cgcaaagtga tctccctgcc cgctccgctg
    13081 cgcggttccg cagtttaccg gcacggcgat cgcacaccag cctggctgag cgaaggccgc
    13141 ctgcaccgca gcctggtatg tgagtgcgaa gcggtaactg cgggtgaagt gcagtacgca
    13201 gtagaaaatt taaatgttaa cagcctgctg gatttacgcc gtcgtacccg tgtagggatg
    13261 ggtacctgcc agggcgaact ctgcgcctgc cgggctgccg gactgctgca acgttttaac
    13321 gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct tccttaacga acgctggaaa
    13381 ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa gcgaatttac ccgctgggtt
    13441 tatcagggac tgtgtggtct ggagaaggag cagaaagatg cgctttgata ctgtcattat
    13501 gggcggcggc ctcgccggat tactctgtgg cctgcaactg caaaaacacg gcctgcgctg
    13561 taccattgtc actcgtggtc aaagcgcgct ccatttctca tctggatcgc tggatttgct
    13621 gagccatctg ccggatggtc aaccggtgac agacattcac agtggactgg aatctttgcg
    13681 tcagcaggca cctgcccatc cttacagcgt tctcggccca cagcgcgtgc tcgatctcgc
    13741 ttgccaggcg caagcattaa tcgctgaaag cggtgcgcaa ttacagggca gcgtagaact
    13801 ggcccaccag cggattacgc cactcggcac tctgcgctca acctggctaa gttcgccaga
    13861 agttcctgtc tggccgttgc ccgcgaagaa aatatgtgtg gtcgggataa gcggcctgat
    13921 ggattttcag gcgcatcttg cggcggcttc attgcgcgaa ttggatctta aagttgaaac
    13981 cgcagaaatt gaactgccgg aactggatgt gctgcgcaac aacgccaccg agtttcgcgc
    14041 ggtgaatatc gcccgtttcc ttgataacga agagaactgg tcgctgttac ttgatgcgct
    14101 tattcctgtc gccaatacct gcgaaatgat cctgatgccc gcctgcttcg gcctggctga
    14161 tgacaaactc tggcgttggt tgaacgaaaa actaccttgt tcattgatgc ttttgccaac
    14221 gctgccgcct tccgtgctgg gcattcgtct gcaaaatcag cttcaacgtc agtttgtgcg
    14281 ccagggtggc gtgtggatgc caggcgatga agtgaaaaaa gtgacctgta aaaatggcgt
    14341 agtgaatgaa atctggaccc gcaatcacgc cgatatcccg ctgcgcccac gatttgcggt
    14401 gcttgcgagc ggcagtttct ttagcggcgg actggtggca gaacgtgacg gcattcgcga
    14461 gccgattctc ggccttgatg tgctacaaac cgccacgcgg ggtgaatggt ataagggcga
    14521 tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc actgatgagg cactacgccc
    14581 gtcgcaggca ggtcaaacca ttgagaacct gtttgccatc ggttcggtgc tgggcggatt
    14641 tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc gtcagtgctt tacatgccgc
    14701 tcaacagatt gcccaacgcg caggaggcca acaatgaacg acaccagctt cgaaaactgc
    14761 attaagtgca ccgtctgcac caccgcctgc ccggtgagcc gggtgaatcc gggatatccg
    14821 gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt tgaaagatgg cgcactgtat
    14881 gacgaggcgc tgaaatattg catcaactgc aaacgttgcg aagtcgcctg cccgtccgat
    14941 gtgaagattg gcgatatcat ccagcgcgcg cgggcgaaat atgacaccac acgcccgtcg
    15001 ctgcgcaatt ttgtgttgag tcataccgac ctgatgggta gcgtttccac gccgttcgca
    15061 ccaatcgtca ataccgctac ctcgctgaaa ccagtgcggc agctgcttga tgcggcgtta
    15121 aaaatcgatc atcgccgcac gctaccgaaa tactccttcg gtacgttccg tcgctggtat
    15181 cacagcgtgg cggcccagca ggcacaatat aaagaccagg tggctttctt tcacggctgc
    15241 ttcgttaact acaaccatcc gcagttaggc aaagatttaa ttaaagtgct caacgcaatg
    15301 ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg gcgtaccgct gatcgccaac
    15361 ggctttaccg ataaagcacg taaacaggca atcacaaatg tagagtcgat ccgcgaagca
    15421 gtgggagtaa aaggtattcc ggtgattgcc acctcctcaa cctgtacctt tgccctgcgc
    15481 gacgaatacc cggaagtgct gaatgtcgac aacaaaggct tgcgcgatca tatcgaactg
    15541 gcgacccgct ggctgtggcg caagctggac gaaggcaaaa cgttaccgct gaaaccgctg
    15601 ccgctgaaag tggtttatca cactccgtgc catatggaaa aaatgggctg gacgctctac
    15661 accctggagc tgttgcgtaa aatcccgggg cttgagttaa cggtgctgga ttcccagtgc
    15721 tgcggtattg cgggtactta cggtttcaaa aaagagaact accccacctc acaagccatc
    15781 ggcgcaccac tgttccgtca gatagaagaa agcggcgcag atctggtggt taccgactgc
    15841 gaaacctgca aatggcagat tgagatgtcc acaagtcttc gctgcgaaca tccgattacg
    15901 ctgctggccc aggcgctggc ttaaactcct ttctgatgcc cggtaagcat gttgttaccg
    15961 ggcatttttg cgtacacgat tccgtgccca atgtatgcgt tgcaacgcag tgaaaattcc
    16021 tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca aaggcctggg ataaccgtaa
    16081 tgtcgcgtca tcataaatat caggtgacgg acaaccatga ccgaatcaac aacctcctcc
    16141 ccgcatgatg cggtgtttaa aacctttatg ttcacacccg aaaccgctcg ggattttctg
    16201 gaaatacatc taccagagcc gctgcgcaag ctttgcaacc tgcaaacttt acgcctggaa
    16261 cccaccagct ttattgaaaa aagtttacgc gcttactact cggatgtttt gtggtctgtg
    16321 gaaaccagcg acggtgaagg ctatatctac tgcgtgattg aacatcaaag ctctgcagaa
    16381 aagaatatgg cttttcggct aatgcgctat gccactgccg ccatgcagcg tcacctggat
    16441 aaaggctatg acagagttcc gctggtggtg ccattgctgt tttatcatgg cgaaacctcg
    16501 ccctacccgt actcactcaa ctggctggat gagtttgacg atccgcaact tgcccggcag
    16561 ttgtacaccg aagcttttcc gttggtggat atcaccatcg tacctgacga tgagatcatg
    16621 caacatcggc gtatagctct gctggaactg attcaaaagc atattcgcga ccgcgattta
    16681 atcggtatgg tcgacaggat caccacgctt ttggttagag gcttcactaa tgacagccag
    16741 ctacaaacgc tgtttaatta tctgctgcaa tgcggcgata cctcccgttt cacccgcttt
    16801 attcaggaga ttgccgaacg ttcaccacta caaaaggaga gattaatgac tattgctgaa
    16861 cggctgcggc aggaaggaca tcaaattggc tggcaggaag gtaaaattga aggttggcag
    16921 gaggggaaat tggaggggtt gcagaaaggc aaagtagaag gtatgcatga acaagccatt
    16981 aaaattgcct tgcgcatgct ggaacagggc tttgaacgtg agattgtgct ggcgacaacc
    17041 caactcactg atgctgatat tccgaacggt cattaaaagg agagattaat gactattgct
    17101 gaacggctac ggcaggaagg acatcaaatt ggctggcagg agggtaaatt agaaggtttg
    17161 caggaaggca tgcatgaaca agccattaaa attgctttgc gcatgctgga acagggcatt
    17221 gatcgtgacc tggtgctcgc ggccacccag ctaagcgaag ccgatctggc agcgaataac
    17281 cactaattaa cacaggccca cagccgatcc ccatgggcct ttgatatcaa taactacctt
    17341 ttatgcgtgg cccatttttg cctgatttaa acatcgccag tcgttgatcc agggcatcgc
    17401 tgtagagcat cgtgtcaacg ccaacagcga caaagttcgc tccccacgcc aggcattgct
    17461 gcgccatatc aggagccaca gccagaaaac cagccgcttt acccgcagca cggatccgcc
    17521 gaatgctggt ttcaataatt cgctgcactt ccgggtgccc ggcgttatcc gggtagccca
    17581 acgaggcaga aagatccgca ggtccaataa acacgccgtc aatcccttcg acgtcgagga
    17641 tttcgtccag gttatccagt gctgttttac tttccacctg caccaacaga caaagcgaat
    17701 cgttgacttg cgccatgtaa ttctcaatgc gtccccagcg cgcagcccgt gccacactgg
    17761 ccccgacacc acgctcaccg tagggaggat agcgcgtggc agacaccacc tgacgtgcct
    17821 gatcggcagt atcgaccatc gggatcagta gagtttgcgc gccaatatcc aggacttgtt
    17881 taatcagcga cttactgcct tccaccggac ggatcacggg ttggctggca tagggcgcta
    17941 ccgcctgtag ctggtgataa agatcctgaa tggtgtttgg cgcgtgctcc ccgtcaatca
    18001 gcaaccagtc ataaccggag gtggcggcaa tttctgccat ataagaagta gtcgagctta
    18061 accacagacc aatttgcact tcgcccttgc gtaaacgttc tttaaaggga ttggttaata
    18121 atgcgttcat ggtgttcctt aacctattaa tgatgtgcca cgtcggtctg cgcaacagtg
    18181 cgatttacac gcagagtgaa aataatcagc gaaccgatga ccgccaccgc tgccagcgtc
    18241 agtaatcccg ccgcatcgct ggcaaacaac gtttctgctt tcacgcgcag gatcggggca
    18301 ataaacccac ccactgcacc aaacaggttt acaaaaccaa tccccgccgc cagcgccgta
    18361 ccggaaagca gttgtgtcgg catcgtccag aacaccggct gcacggcaat aaacccgatg
    18421 gctgcaacac acagcgctac gatcgccagt actggagaaa gcagcccgga gagaccaata
    18481 ccaatgcctg ccgccagtaa tgtcagcgcc gcgacattac gccgttcgcc ggttttatcg
    18541 gaatagcgcg gaataagcca ggtcccaaac aaggccgcga cccacggaat ggcggtgacc
    18601 accgacgccg taaagcccac ttttgtcccc aacaatgcag caacctgcgt cggcaggaag
    18661 aaaattaatc cgtaaaccgc cacctgaatg gtcaggtaga taatcgccag ttgccagact
    18721 cgcccattac gcagcgcatc gctcagtcga gaagtcactt tctgttgttc ttcacttgcc
    18781 agttggttga taagcaacgt tttttcttct ttactcagaa aacgtgcctg ctccggcgtg
    18841 tcatcaagcc aaaagaatgt gaatacccca gcgccgactg ccagcaatcc ttcaatcaca
    18901 aacatccaga accagccggg atgccccata aatccatgca tctccagcag cgcgccggaa
    18961 agcggtgatc ccagtgttaa cgccaacggt gcgcccatat agaacagccc cataatgctg
    19021 gcgcgattac gctgcggaaa ccattgcgag gtgagataaa tcataccagg gaagaacccg
    19081 gcctccgcag caccaagcag agtgcgaacg atcagaaatt tcgcttcagt atcggcccat
    19141 gccatggctg ccgaaagaaa tccccacagc agtgttgtcg taccaatcca ggttctggcc
    19201 cccagtttgc gcatcaaaag attcgccgga acacccagaa acgcatatac cacaaagaaa
    19261 atgcctgctc ccagcgcata agcttcatta ctcagcccgg tatcaatctg gtaggtttgt
    19321 ttggcaaaac cgatattcga acggtcgaga aacgccagca catacagcgc caacttgaca
    19381 tcctccacgc cctgaagaac gtggatccct gctacgttca ggctgtcgcc tgaatcattt
    19441 cggtgggttc ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg
    19501 cgtgccccgc cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac
    19561 atacctggca tctgaattga ctttgtgaca ggcgattttc tttcgcagta tgaccacagc
    19621 acgcgcaacg ctggcttgtg tacgctggcg gaacagcaag caactgaccg ccactccaga
    19681 gctgcttgta ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt
    19741 ttaaacctga ttttgcccgg acattgcgcc ccggc
//
LOCUS       DAYPHN010000083        19764 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000083 DAYPHN010000000
VERSION     DAYPHN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.083"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..587
                     /locus_tag="ACG0BW_002823"
     CDS             <1..587
                     /locus_tag="ACG0BW_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFK7259761.1"
                     /translation="ASVSDSSDTADIFPGTPFPLLFEIEVSSCTGNNTNCNSVRFNFN
                     LNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDV
                     EYYFEPVTIQESGTSILKNDLENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHE
                     GSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(625..1524)
                     /gene="nhaR"
                     /locus_tag="ACG0BW_002824"
     CDS             complement(625..1524)
                     /gene="nhaR"
                     /locus_tag="ACG0BW_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFK7259762.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(1590..2756)
                     /gene="nhaA"
                     /locus_tag="ACG0BW_002825"
     CDS             complement(1590..2756)
                     /gene="nhaA"
                     /locus_tag="ACG0BW_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFK7259763.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3342..3494
                     /gene="mokC"
                     /locus_tag="ACG0BW_002826"
     CDS             3342..3494
                     /gene="mokC"
                     /locus_tag="ACG0BW_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFK7259764.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3598..4728)
                     /gene="dnaJ"
                     /locus_tag="ACG0BW_002827"
     CDS             complement(3598..4728)
                     /gene="dnaJ"
                     /locus_tag="ACG0BW_002827"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFK7259765.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4817..6733)
                     /gene="dnaK"
                     /locus_tag="ACG0BW_002828"
     CDS             complement(4817..6733)
                     /gene="dnaK"
                     /locus_tag="ACG0BW_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFK7259766.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7109..7513
                     /gene="yaaI"
                     /locus_tag="ACG0BW_002829"
     CDS             7109..7513
                     /gene="yaaI"
                     /locus_tag="ACG0BW_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFK7259767.1"
                     /translation="MKSVFTLSASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7539..8252
                     /gene="msyB"
                     /locus_tag="ACG0BW_002830"
     CDS             7539..8252
                     /gene="msyB"
                     /locus_tag="ACG0BW_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFK7259768.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8401..8967
                     /gene="satP"
                     /locus_tag="ACG0BW_002831"
     CDS             8401..8967
                     /gene="satP"
                     /locus_tag="ACG0BW_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFK7259769.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(9002..9589)
                     /gene="mog"
                     /locus_tag="ACG0BW_002832"
     CDS             complement(9002..9589)
                     /gene="mog"
                     /locus_tag="ACG0BW_002832"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFK7259770.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(9704..10657)
                     /gene="tal"
                     /locus_tag="ACG0BW_002833"
     CDS             complement(9704..10657)
                     /gene="tal"
                     /locus_tag="ACG0BW_002833"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFK7259771.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            10936..12366
                     /gene="yaaJ"
                     /locus_tag="ACG0BW_002834"
     CDS             10936..12366
                     /gene="yaaJ"
                     /locus_tag="ACG0BW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFK7259772.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMALIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            12436..13212
                     /gene="yaaA"
                     /locus_tag="ACG0BW_002835"
     CDS             12436..13212
                     /gene="yaaA"
                     /locus_tag="ACG0BW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFK7259773.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFNDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13365..13661)
                     /gene="yaaX"
                     /locus_tag="ACG0BW_002836"
     CDS             complement(13365..13661)
                     /gene="yaaX"
                     /locus_tag="ACG0BW_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFK7259774.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13875..15161)
                     /gene="thrC"
                     /locus_tag="ACG0BW_002837"
     CDS             complement(13875..15161)
                     /gene="thrC"
                     /locus_tag="ACG0BW_002837"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFK7259775.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWLPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(15162..16094)
                     /gene="thrB"
                     /locus_tag="ACG0BW_002838"
     CDS             complement(15162..16094)
                     /gene="thrB"
                     /locus_tag="ACG0BW_002838"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFK7259776.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMALEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(16096..18558)
                     /gene="thrA"
                     /locus_tag="ACG0BW_002839"
     CDS             complement(16096..18558)
                     /gene="thrA"
                     /locus_tag="ACG0BW_002839"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFK7259777.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18584..18703)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18639..18704)
                     /gene="thrL"
                     /locus_tag="ACG0BW_002840"
     CDS             complement(18639..18704)
                     /gene="thrL"
                     /locus_tag="ACG0BW_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFK7259778.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18918..19604)
                     /gene="yjtD"
                     /locus_tag="ACG0BW_002841"
     CDS             complement(18918..19604)
                     /gene="yjtD"
                     /locus_tag="ACG0BW_002841"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFK7259779.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         4793 a         5190 c         4788 g         4993 t
ORIGIN      
        1 tcgcatctgt atcagatagt tctgacaccg cagatatttt tccaggaact ccttttcctt
       61 tgctgttcga aattgaagtg agttcatgca cagggaataa tactaattgt aatagcgtcc
      121 gctttaattt taatttaaat actattttac aggtaaattt aatgacctgt ggttttgaag
      181 atcaatccat cgacactgga cgatttgtac tgtctcgtat caataatgaa aattatcagt
      241 ttcatcatat aagatttgac tgtatccagg gtgagcaagg tgaattagtt tttgaaccca
      301 gtgatgttga atattacttt gagccagtga cgatacagga gtcaggaacc agcatattaa
      361 aaaatgattt agaaaacagt gaggacggtg cgggtcaaat aggctttcaa ctctccaatg
      421 atggtacgaa tgaaatccag tatggtaaaa gcaattacta ctcttttcag catccccatg
      481 aaggaagtaa tcagattcct ctatttattc gcccgcgtac atatggaaac aatgtctcaa
      541 gcgggcaaat aatgagccga gtcaaaatcg tggtaatgta caactaatct atctccccca
      601 ccttaacttt gggggagctg ccgattaacg tgccgctggg ctaaaaagcg cagaataatc
      661 cgtattgcag attcgctgta ccgccgggtg ctgaatcatc cgctcagcaa aaatagcatg
      721 gtactcttcc atcacatttt cgacgcgacc aatttctacg acagttttat cggcataaaa
      781 gtcatatgca taaagcgttg gggcaacgaa gattgcattg tgcatcgcac caaaagcttt
      841 catcaacgcg gcatcatcaa actcgccgag gatttctacg tttaatccct gggagttaaa
      901 ccagttaagc aatttgcgcc ctaacattga acgtcgccca ggaatcaaaa gccgccgttc
      961 ttccagacaa gccgggaacg gtttttccgg tggtggattt gtacaccaga aactcacgcc
     1021 acattcgcca attctcacgg agaacagacc ttcctgctgc gtagaatcta tcggacagtc
     1081 ggaaatgatc atatccagtt tatgctgact taattgctcc agcagcattt cgtgggtgga
     1141 ttcgaagcag cgaagatgaa tcggctcgcc ttctaccact gcggcgttaa gtacgctact
     1201 gaccaggcgt ttagaaagtg catcagccac gccaacgtca aacaataaat tggattcttt
     1261 gcgatagttc acgatatcca gcatttcctg gcttaaggtg aacattttat cggcatagcg
     1321 atagaccagc tctcccagct cgctgggctc gaggccacgt cccttgcgct taaataattt
     1381 gccttgcagg cgctcttcca gcgcccgaat ctgtccggta atggtttgtg gtgttaaata
     1441 aagcgcctcc gctgcgccaa ccacggaacc ttctttatag acatgccaga agtaatacaa
     1501 gtggttgtaa ttgatatgag acatgctcat ttctctccct gataacaatg aaaagggagc
     1561 cgtttatggc tccccggtaa accgtcctgt caaactgatg gacgcaaacg aacgcgtaac
     1621 cagctgtatc caattaccgc cgaagagata gaaccgacca ggataccgag tttcgcccag
     1681 ttaatcagtt ctggatctac gctaccaaag gccaggctgg caataaagat agacatagta
     1741 aaaccgatac cgcacaggat ccccaccgcc ataatttgct gataagtcgt tccctcaggc
     1801 agatgcgcca atttcaaacg cagcgccaac cagcagaaca gactaatccc cagcggtttg
     1861 ccaatcagca agccagcgat gatccccaat ggcagaatgg aggtcaagcc atccagcgtg
     1921 acgccttgca gtgaaacgcc agcattagca aatgcaaaca gcggtaaaat cagatacgcc
     1981 acccacgggt gcaacacatg ctccagtcgc ttcgctggag aacgcccatg cttctctttc
     2041 aaaggaataa agaagccgac aattaccccc gccagggttg cgtgaacccc cgatttcaac
     2101 accgcagtcc acaacaccac gccaaccaga atatagacgc ccgtgcggcg tacaccacac
     2161 agattcaata ccgcgagtac cgcaattgct acagccgcga caccaagaga ggccatcgat
     2221 aagtcattag tgtagaacaa tgcgatgata atgatggccc caagatcgtc gataatagcc
     2281 agagccatca aaaagatctt cagcgctaac ggaacacgac ttcccaacag cgccagcaca
     2341 ccaagtgcaa aggcaatgtc agtagccgcc gggatcgccc acccttcgcg ggtaatcgga
     2401 tcggcatagt taaaggccag atagagcaat gccggcacaa tcatcccacc aatagcggcg
     2461 ataactggaa atgcggcctg gcgtaagctg gctagcgatc cttgcatcag ttcacgttta
     2521 acttccaaac cgactaacag gaaaaatacc gccatcagcg cgtcatttat ccataacagc
     2581 atgtttttgt tgatttcgag tgaaccaacc cggagctgaa ccggcgtctc cagaaagtcg
     2641 tgataccatc cactggttgc gccgctattg gccataatca tcgccaggat agcggcaatg
     2701 ataagaataa tgcctcccga ggcatcactg ctaaagaatc gatgcagatg tttcactttt
     2761 tatttctctt tcaggtgaat agatcgtttt tactatttta ccccgcacga atcatcgtta
     2821 aaatcagatt atagtgtagg aataattcgt tttttacgaa ccattgaagg atttgcgtca
     2881 tcagtgatga gatgagtttt tgacggctgt aagcgctgac gtgtgaccct aagccctgat
     2941 aatcatagct taaggtcagg atttctgagg gattaattag gaaaagtaat aattaatttg
     3001 ttgtcagtct gcgatgtttg ttaggccaat atttcaaaaa taatccagtt tgtcgacaat
     3061 aattgcgatg aagagataaa ttaaagttaa ataattaaat cattaaaaaa ttacgccata
     3121 aaaatgcgag gcgtcttcag gattagaatc gataaaacag aatgggttaa agagagcgat
     3181 ataatagcgg cgggtgcttg aggctgtctg tctcaggcat tagctgaacg gcagatagag
     3241 aaaagccccg agtgatattt taccatcaac ccgaggcccc ctatatgctg aacacatgta
     3301 gagtgcctct tactgaccgt aaggtcaagg agaagagagc aatgaagcag cataaggcga
     3361 tgattgtcgc cctgatcgtc atctgtatca ccgccgtagt ggcggcgctg gtaacgagaa
     3421 aagacctctg tgaggttcac atccgaactg gccagacgga ggttgctgtt ttcacggctt
     3481 acgaatccga gtaagagcaa cggcggggag tgatccccgc cactttacag gtgctcgcat
     3541 atcttcaacg caccctattt ttacccaagc ctgcccacgg gcaggctttt ggggaggtta
     3601 gcgagtcagg tcgtcgaaaa acttcttcac accatcaaag aagctctttg aacgcgggct
     3661 gttgtgctcg ccggttgggc caccgaagct ttcctgcagc tcttgcagca gctgtttctg
     3721 cttctcgttc agacctaccg gtgtttcgac gacaacgcgg cacagcaaat caccctgtgc
     3781 gccaccgcgg acagacttga cgcctttacc gcgcatacgg aacagcttac cggtctgggt
     3841 ttcgccaggc actttcagtt tgacgcgacc atcaagggtc ggtacttcga tttcgccacc
     3901 cagcgccgcc atagcgaagt tgatcgggac ttcgcaatag aggttgttgc cttcacgctc
     3961 gaaaatcggg tgctgtttaa cctgaacctg aacgtacaga tcgcctgccg gtgcgccatg
     4021 ctcgcccgct tcaccttcgc ccgcaagacg gatgcggtct ccagtgtcca cccctgccgg
     4081 gattttaacg gacagcgttt tgctgcgctc aacacgacca tgaccatgac atttgttgca
     4141 cggatctttg atcagcgtac cccggccctg acagtgtgga caggtctgct gcacggcaaa
     4201 gaaaccctgg cgcatctgca cctgaccaga accatgacag gtcggacagg tctgcggctg
     4261 tgtacctggt tttgcaccgc taccgtggca aacgtcacac tcttccagag tcggaatgcg
     4321 gatctctttg gtcacgccac gtacagcttc ttctagggtg agctccatgt tatagcgtaa
     4381 atcagcaccg cgcgccgcac gttgacgacc acgtccgccg ccaaaaatat cgccgaaaac
     4441 gtcaccaaaa atatcgctga agtctgcgcc gccgccaaaa ccgccgccgc ccatgccgcc
     4501 ttgctcaaac gcagcatgac catactgatc gtatgccgca cgtttttgcg agtcggtcag
     4561 aacttcataa gcttccttga tctctttaaa tttcgcctcg gcctctttgt caccctggtt
     4621 acggtccggg tggtatttca tggccaggcg tttgtaggcc tttttgattt cacgctcttc
     4681 cgctgttttg gaaacgccta aaatctcgta ataatcttgc ttagccatct tttttaaatt
     4741 gcccctagat gaatgcacgg gcggagagga aatacccctt cgcccgtgtc agtataatta
     4801 cccgtttata gggcgattat tttttgtctt tgacttcttc aaattcagcg tcgacaacat
     4861 cgtcatcttt cgcgttgttt gcagaagcat cagcaccggc agtctgctgc tgggcatgtt
     4921 gctgctgggc gatttccatc agtttctggg aaacctgtgc cagttcctgc attttcgctt
     4981 cgatagcggc tttgtcttca cctttcagag cagtttccag tgcagtcagt gcagactcga
     5041 tagcagtttt gtcgtcagcc ggcagtttgt cgcctgcttc ttcaacctgc ttacgggtgc
     5101 tgtgcagcag atggtcgccc tggttgcgag tctgtaccag ctcttcaaac ttacggtcag
     5161 cttcggcgtt agcttctgcg tcgcgtacca ttttctggat ttcatcttcg ttcagaccag
     5221 aagaagcctt gatggtgatc ttctgctctt taccgctgtt tttatctttc gcggaaacgt
     5281 gcaggatacc gtcagcatcg atatcgaagg taacttcgat ctgcggcatg ccgcgcggtg
     5341 ccgggttgat accatccagg ttgaactgac ccagagattt gttatcagct gcacgtttac
     5401 gttcaccctg cagcacatgg atggttaccg cagactggtt gtcttcagcg gtagagaaca
     5461 cctggctgtg cttggtcggg atagtggtgt ttttcgcgat cagcgtcgtc atcacaccgc
     5521 ccatggtttc gatacccaga gacagcgggg taacgtccag cagcagtacg tcttttacgt
     5581 caccagtcag aacaccaccc tgaacagcag caccgattgc tacagcttcg tccgggttaa
     5641 cgtctttacg cggctcttta ccaaagaact cagcaacttt cttctgaacc attggcatac
     5701 gagtctgacc accaacgagg ataacgtcgt cgatatcaga tacggacagg ccagcgtcct
     5761 gcagtgcaac tttcagcggc tcaatggaac ggtttaccag atcttcaacc aggctttcca
     5821 gtttcgcacg agtcactttg atgttcatgt gtttcggacc ggtcgcgtct gcagtgatgt
     5881 acggcaggtt aacgtcggtc tgctgagcgg aagacagctc aattttcgct ttttctgccg
     5941 cttctttcag gcgctgcatt gccagcggat cgttgcgcag gtcaatgccc tgatctttct
     6001 tgaattcttc aaccaggtag ttgatcagac ggctgtcgaa gtcttcacca cccagatggg
     6061 tatcaccgtt ggttgccaga acttcgaagg ttttttcgcc gtcaacttcg tcgatttcga
     6121 taatagaaat atcgaaagta ccaccaccca ggtcataaac cgcgatagta cggttgccag
     6181 tacctttgtc cagaccgtaa gccagcgcag ctgcggtcgg ttcgttgatg atacgtttta
     6241 cttccagacc agcgatacgg cctgcgtctt tggttgcctg acgctgagca tcgttaaagt
     6301 atgccggtac ggtgataaca gcttcagtta ccggttcacc caggtaatct tcagcggttt
     6361 tcttcatttt tttcagcact tcagcagaga tctgcggcgg tgccattttc tggcctttaa
     6421 cttcgaccca tgcgtcgccg ttatcagcag caataatttt gaacggcatg atggaaacat
     6481 cacgctgtac ttcttcgtcc tggaagcggc ggccaatcag gcgtttaatc gcaaacaggg
     6541 tattttgcgg gttcgtcact gcctgacgtt tagccggctg accaaccaga gtttcaccat
     6601 cctgggtata ggcaatgata gaaggcgtgg tgcgatcgcc ttcggcgttc tccagtacac
     6661 gaggagtggt gccatccata atcgctacac aagagttggt agtacccagg tcgataccaa
     6721 ttattttacc catctaaacg tctccactat atattcggtc atcatgtggt tgtgagtctg
     6781 taataggggc gaaacgtctg gtttcaactg cccaatttca tttttttgca gactcactca
     6841 ctgcggttga ctactaaatg gggtcgtaac ccacgtcatc aagggggaga tgcaaaaatt
     6901 ttttgtgctt ttgcgcagaa atttcgcgat atgccagaac aagacatgaa actgacagca
     6961 attttattca tatagagaat gaatagcagc caagacattc atagaaaaat tgtaataaaa
     7021 agaaaatatt acgtacctga atcttctctt tatcagcagt aaactagtgg gtattcatcc
     7081 cctacctctc cccactaaga gaatccttat gaaatccgtt tttacgcttt ccgccagcct
     7141 ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaaa tcctcggcgt
     7201 cattgcaatg ccacgtaacg aaaccaacga tctggcgctg aaacttcctg tttgccgaat
     7261 tgttaaacgc atacaactct cagccgacca tggcgatttg cagttaagcg gtgcatcggt
     7321 ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag aaataaaaga
     7381 agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc
     7441 aaaaatcacc ttttccggcc atacggtgaa ctcatcggat atggccacgc tgaaaattat
     7501 cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg
     7561 attcagatct ggattttctc cagcattgta gtgaggaaca attggcaaat ttcgcccggc
     7621 tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg cgcaacgaac
     7681 tgtttaaatc gatggaagga catcccgagc aacaccgccg caactggcag ctgattgccg
     7741 gagaattaca acattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat
     7801 tgtatcgggc cattttgctc gatgtttcaa aacgattgaa gctgaaagcc gacaaagaga
     7861 tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga
     7921 aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc
     7981 tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggc gtgtcgcatt
     8041 tgctttccag ccaattgacc cgcattttac gcacccacgc agcaatgagc gtacttgggc
     8101 atggtttgct tcgcggcgcg gggctgggag gccctgtagg cgcggcacta aatggggtta
     8161 aagcggtcag cggcagcgcc tatcgcgtga cgattccagc cgtactgcaa atcgcctgcc
     8221 tgcgccggat ggttagtgcc actcaggtct gataccagaa accggggaaa attttcattt
     8281 tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa
     8341 cactacagtt attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt
     8401 atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc
     8461 accattctgc ttaacctgca caacgtgggt tatttcgctc ttgacggtat tattcttgcc
     8521 atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa
     8581 ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt
     8641 gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt
     8701 gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac gctgaaaggc
     8761 gcacgcgttc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc
     8821 ggtaacattg ccggtaacgc cgcaatcatc cactttgccg gctggattgg tctgatctgc
     8881 ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc
     8941 gttctgccga ttggtgaatc gcactaattg ttttgagatc cccccgctcg gggggatttt
     9001 tttattcgct aacttcgcgt cttgcactct tcggtctgaa tgcagcaacc acttccggtg
     9061 ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac
     9121 cgtgcaccac aacgttaccc tcagcgtcct ttacaccttc cagcgtctct ttaatcgact
     9181 tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacaccc acctgacgcg
     9241 aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagcctg
     9301 gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc
     9361 cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg
     9421 tttgctcgat gatcgcctgc tcgtcgggga ttaagcgggt ttccagttca aacggcgtgg
     9481 ttagcgccga tgtcagccat tcttccaacg cagggatgcc tttatcctga taaacgccgc
     9541 tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc
     9601 cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc
     9661 agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga
     9721 tcattttttc cagtttttct tggtcaacag caaacttacg gataccttcc gccagtttat
     9781 ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg
     9841 gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct tcgctctcag
     9901 ccagctcttt cagcagtgcc ggagcgatgg tcagacggtc gcaacctgcc agttccagaa
     9961 tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt
    10021 agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt
    10081 tcttgtcggt attcgctttg taccagtcaa gaatacggcc aacaaatggc gagatcagga
    10141 acacgcccgc ttccgcacaa gcacgagcct gagcgaagga gaacagcagt gtcaggttac
    10201 agttaatacc ttctttttcc agctgttctg cagcacggat accctgccag gtagaagcca
    10261 gtttgatcag aatacgatcg ttgctaatcc ctgcatcgtt gtagagtttg atcaggcgtt
    10321 ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact tcagttgaga
    10381 tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcggtcg
    10441 cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat
    10501 caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg
    10561 tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg
    10621 tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc
    10681 ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc
    10741 gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat
    10801 cgcccgccat ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt
    10861 agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca
    10921 ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg
    10981 gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg
    11041 cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca
    11101 ggcggtttaa cctcatttca atcattgtgt accagtcttg cggcgcgcgt gggtagcggc
    11161 aacctggctg gcgttgcgct ggctattacc gccggtggac caggtgccgt cttctggatg
    11221 tgggttgccg cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt
    11281 tataaagaac gtgacgttaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc
    11341 gggctgggga tgcgctggat gggcgttctg ttcgccctct ttttgctcat cgcctacggc
    11401 ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt ttcttttgat
    11461 tttcccccgc tggtgacagg cattattctt gctgtctttg ctctgctggc aatcactcgc
    11521 ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gttaatctgg
    11581 gtactgacca gcctggtaat ttgcgtaatg aatatcgggc aacttcccca cgtcatttgg
    11641 tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc
    11701 ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga ggcaggaatg
    11761 gggtcgacgc caaacgcggc agcggcggct gcgtcctggc ctccgcatcc cgcagcgcaa
    11821 ggaattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc
    11881 atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt
    11941 atccagaagg cgatgcgggt gttaatgggt tcctggggtg cggagtttgt taccctcgtg
    12001 gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc
    12061 ttcttcttac gcctgaacaa ccctaaagcg atctggtgtc tgcgaatctg caccttcgca
    12121 acggtcatcg gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcatt
    12181 atggcctgca tggctattac caatttgacc gccatattac tgctctcgcc tgtggttcat
    12241 accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg
    12301 ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag
    12361 gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc
    12421 ctgcaaggac tggatatgct gattcttatt tcacctgcaa aaacgctcga ttaccaaagc
    12481 ccattgccca ccacgcgcta tacgctgccg gagctgttag acaattccca gcagttgatc
    12541 catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa
    12601 ctggcgggta tcaacgccgc tcgctttaat gactggcagc cagatttcac gccggagaat
    12661 gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc
    12721 ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat
    12781 ggcgtactac gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt
    12841 gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg
    12901 aacgaggcgc tcgcagcaca aggcgataat gtggtgataa atctggcgtc agatgagtat
    12961 tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccagt gttcctcgat
    13021 gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg
    13081 agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc
    13141 gaaggttact tctttgatga agcttcctcc agcaatggtg aactggtgtt taaacgctac
    13201 gagcagcgtt aagtttgcag taaatgccgg atgcggcgtg aacgccttat ccggcctaca
    13261 aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac gcgccagcgt
    13321 cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg
    13381 cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg cggcggtggc
    13441 ggcggtccat atgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg
    13501 tggtcgcgcc agtgaccgcc atcccagtaa tagccacgat tatcacgatc gcctatctgt
    13561 aattttactg acgggactag cgtaatttcc gcagcctgcg ctgccatggg agcgaccaga
    13621 accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat
    13681 tgcccgttat gggtcgatat ccggagatta cgcgatgaga acggcagttg ttattctccg
    13741 caatcctaat ctctaagtta ccgcatttat tgagaatttc tcctttttcc ctgtcgtttt
    13801 tctccatatt ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag
    13861 ataatgaata gatgttactg atgattcatc atcaatttac gcaacgcagc aaaatcggcg
    13921 ggcaggttat gcgaaagcaa gggtaaatca gcacgttctg ccagctcttt tggcagatcc
    13981 aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg
    14041 aggaacaagc catattcgcc tggatgcaac tggtcacgca gcgcacgata agctacggca
    14101 gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt
    14161 tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac
    14221 tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc
    14281 tgagtcgctt tgggtaacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg
    14341 ttggtcgcag caataaaacg tttcaccggc agaccgagtg acttcgccag cagacccgcc
    14401 gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcttcctgc
    14461 ggcaactgcg ccacggcttc aaagtagtag caaatctgcg ccagcaaacg gctgatgtta
    14521 atcgagttag ctgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc
    14581 ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc aatattgccg
    14641 cccaatgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata
    14701 accactttca cattcggcaa accgtagaaa gcatgagcca ctgccgctcc ggtatcaccg
    14761 gaggtcgcgg tcagaatggt cactggctta tcgcccgcaa tatgggtcag catttgtgcc
    14821 ataaagcgac cgccgaaatc tttaaatgca agcgttggcc cgtggaacaa ctccagacaa
    14881 ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgtacgcgc
    14941 tcttccagga tttcctgcgg gatttcatca ccaataaacg ccgagaggat cttcgcactg
    15001 cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg
    15061 tcgtgcggga aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag
    15121 ctgacctgct cattgtgatc tttaagattg tagagtttca tttagttttc cagtactcgt
    15181 gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc
    15241 ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacacag agcgaacaaa
    15301 gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc
    15361 gcctgccgga agcctggcag taaccgttca cggtagggtt cagcgataac atctttcatc
    15421 agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaataaagcc tgccagatgt
    15481 cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc cctggcttct
    15541 gccgtcgaga ctttaatccc cggatacgcc agcacccaca gccactcatc aaaccctggc
    15601 acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac
    15661 ggtgccacgt tgtcgtaatg aatgctgccg gagatacgcc cttccagctc gcccatcaaa
    15721 gccagcaaac gggtgtcatt aagcggcttg ccgcagtgtt cattcatcgc catcagcgcc
    15781 gcgaccactg agcaggcgct ggagcctaag cccgagccga tcggcatatt cttttccagg
    15841 gccatcgcca ctggaatttg cttacccagc tcctggcaaa aacgctccca acactgataa
    15901 acgatatttt cccgaggttc tgacggcagc ttatcggcaa agcgtccgag gttattgaga
    15961 ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc atcaacgggt
    16021 gtcaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca
    16081 taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa
    16141 aacaccggca gctgtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg
    16201 ctgataatag tggctataga aggccagggc gttttcgccg tttttcactt tgaacagcgg
    16261 atcgttgcca tccacttcag caatcttcac gcggcagacg ccatcttcat caatattgcc
    16321 aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc
    16381 gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc
    16441 gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg tttcacgagc
    16501 gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg ggtccggttc
    16561 ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa
    16621 cttgccgaag atataagaaa gcgaaccaga aagaatgccg gagaacttca tcaattcatc
    16681 acctgcattg agtagatttt gcaggttctc aataaccggt aatccagccc caacgttggt
    16741 gtcatagagg aatttacgcc gcgatttttc cgccgcatga cgcaacagat ggtagtaatc
    16801 catcgacgag gtgttagcct ttttgttcgg cgtaacaacg tggaaacctt cgcgcaggaa
    16861 gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag
    16921 cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg
    16981 cgccagttct tcctgccagt tttccagatt aaggccatgt acattggtga gcagagcctt
    17041 cgagttggca acaccacaga cacgtaagtc gatatgttta ttcttcagcc agctttgctg
    17101 acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc
    17161 gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc
    17221 atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat
    17281 gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg
    17341 cataccatca cctaccaccg agataatggc cagccgttcc gtcactgcca gcggctccag
    17401 taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca
    17461 gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac
    17521 cacggaaata cgggcgcgtg acatcgctgc aaagacgcgc gccgccatgc caaccattcc
    17581 tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt
    17641 gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg cttgaggatt
    17701 tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg
    17761 gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgactt
    17821 caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt caacgtctgt
    17881 ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgcagcag agtagtcgga
    17941 accgttgcgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga aacctgccat
    18001 cagcaccatg tgatcagccg gaatacgact tgccgcaata cggcgggtgg actcagcaat
    18061 atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg gatcgataac
    18121 ggtaacgttg tgaccgcgcg cttctaatac gccggccata atggcgatcg acattttctc
    18181 gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca aactaatgcc
    18241 atgcaggaca tgttttattt gggcaaattc ctggtcgacg aaagttttca gttgcgccag
    18301 cgggaatccc ggctgggcgg cggcgagtcc cgtcagaagt tcggcaaaaa tacgttcggc
    18361 atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag
    18421 gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt
    18481 gctttccaga atatcggcaa cccgcagaaa acgttctgca tttgccactg atgtaccgcc
    18541 gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac
    18601 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
    18661 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
    18721 gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag tgacctaagt
    18781 caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta tcagacactc
    18841 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
    18901 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
    18961 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gttgtaacca gtcgaccagt
    19021 tttatgtcat ctgccactgc cagagtcgtc agcagtgcca tggctcgttc gcgtaaagct
    19081 tgcagttgat gtttgtctgc cgttgtgtca cttttcgccg gttgttgtat taatgttgct
    19141 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
    19201 atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcaa cccggaatct
    19261 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
    19321 accagttcta ctggcgtagc gtagtaatga tatttcgccc gactgcgcgc agtggtggca
    19381 acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt aatattatca
    19441 ataatatcac cagatccatg tgcgacccag cgagtagctg gctccagatg tgcctgacta
    19501 tcgacaatcc gcagttcgct aaaccccatc gttttcatag cccgcgccgc tgccccaata
    19561 ttttctgctc tggcgggtgc gaccagaata atcgttatac gcatattgcc actcttcttg
    19621 accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
    19681 ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttc
    19741 ctgactgtac taacggttta attg
//
LOCUS       DAYPHN010000084        19734 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000084 DAYPHN010000000
VERSION     DAYPHN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.084"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..351
                     /gene="mepH"
                     /locus_tag="ACG0BW_002842"
     CDS             <1..351
                     /gene="mepH"
                     /locus_tag="ACG0BW_002842"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFK7259780.1"
                     /translation="YRWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAA
                     PIERSELKNGDLVFFRTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRH
                     YVGARRVMTPKTLR"
     gene            479..1060
                     /gene="sodB"
                     /locus_tag="ACG0BW_002843"
     CDS             479..1060
                     /gene="sodB"
                     /locus_tag="ACG0BW_002843"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFK7259781.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1206..2375)
                     /locus_tag="ACG0BW_002844"
     CDS             complement(1206..2375)
                     /locus_tag="ACG0BW_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7259782.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(2541..2630)
                     /gene="cydH"
                     /locus_tag="ACG0BW_002845"
                     /gene_synonym="ynhF"
     CDS             complement(2541..2630)
                     /gene="cydH"
                     /locus_tag="ACG0BW_002845"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFK7259783.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            2929..3954
                     /gene="purR"
                     /locus_tag="ACG0BW_002846"
     CDS             2929..3954
                     /gene="purR"
                     /locus_tag="ACG0BW_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFK7259784.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(3951..4883)
                     /gene="punR"
                     /locus_tag="ACG0BW_002847"
     CDS             complement(3951..4883)
                     /gene="punR"
                     /locus_tag="ACG0BW_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFK7259785.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            4996..6207
                     /gene="punC"
                     /locus_tag="ACG0BW_002848"
     CDS             4996..6207
                     /gene="punC"
                     /locus_tag="ACG0BW_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFK7259786.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            6498..7646
                     /gene="cfa"
                     /locus_tag="ACG0BW_002849"
     CDS             6498..7646
                     /gene="cfa"
                     /locus_tag="ACG0BW_002849"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFK7259787.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIHVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(7686..8327)
                     /gene="ribE"
                     /locus_tag="ACG0BW_002850"
     CDS             complement(7686..8327)
                     /gene="ribE"
                     /locus_tag="ACG0BW_002850"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFK7259788.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            8542..9915
                     /gene="mdtK"
                     /locus_tag="ACG0BW_002851"
     CDS             8542..9915
                     /gene="mdtK"
                     /locus_tag="ACG0BW_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFK7259789.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(9956..11212)
                     /locus_tag="ACG0BW_002852"
     CDS             complement(9956..11212)
                     /locus_tag="ACG0BW_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFK7259790.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSMVKGDSVTFNVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            11521..11597
                     /locus_tag="ACG0BW_002853"
     tRNA            11521..11597
                     /locus_tag="ACG0BW_002853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11555..11557,aa:Val,seq:gac)
     gene            11602..11678
                     /locus_tag="ACG0BW_002854"
     tRNA            11602..11678
                     /locus_tag="ACG0BW_002854"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11636..11638,aa:Val,seq:gac)
     gene            11786..12091
                     /locus_tag="ACG0BW_002855"
     CDS             11786..12091
                     /locus_tag="ACG0BW_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFK7259791.1"
                     /translation="MATLLQLHFAFNGPFGDAMVEQLEPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12217..13821
                     /locus_tag="ACG0BW_002856"
     CDS             12217..13821
                     /locus_tag="ACG0BW_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFK7259792.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQIINAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(13833..14645)
                     /gene="ydhT"
                     /locus_tag="ACG0BW_002857"
     CDS             complement(13833..14645)
                     /gene="ydhT"
                     /locus_tag="ACG0BW_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFK7259793.1"
                     /translation="MIITRAGLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(14649..15434)
                     /gene="phsC"
                     /locus_tag="ACG0BW_002858"
     CDS             complement(14649..15434)
                     /gene="phsC"
                     /locus_tag="ACG0BW_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFK7259794.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVALHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYIGVMFGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(15431..16099)
                     /locus_tag="ACG0BW_002859"
     CDS             complement(15431..16099)
                     /locus_tag="ACG0BW_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFK7259795.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHYFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16163..16801)
                     /locus_tag="ACG0BW_002860"
     CDS             complement(16163..16801)
                     /locus_tag="ACG0BW_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFK7259796.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDHADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(16813..18915)
                     /locus_tag="ACG0BW_002861"
     CDS             complement(16813..18915)
                     /locus_tag="ACG0BW_002861"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFK7259797.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLNIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAIEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPLLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(18936..19562)
                     /locus_tag="ACG0BW_002862"
     CDS             complement(18936..19562)
                     /locus_tag="ACG0BW_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFK7259798.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECAYACPTGALKIIEWKDITV"
BASE COUNT         4885 a         5120 c         4640 g         5089 t
ORIGIN      
        1 tatcgttggg gtggcagctc accgcgtacc ggttttgatt gcagcggcct ggtttattac
       61 gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg
      121 cgcgatgcag cgccaatcga acgtagtgaa ctgaaaaacg gcgacctggt ctttttccgt
      181 actcagggac gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt
      241 cagtcaccgc gcacgggtca ggaaattcaa atcacttctc tcagtgaaga ctactggcag
      301 cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta aaactttacc
      361 ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta
      421 tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat
      481 gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc
      541 ggaaaccatc gagtatcact acggcaagca tcatcagact tatgtcacta acctgaacaa
      601 cctgatcaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc gcagctctga
      661 aggtggtgta ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct
      721 ggcaccgaac gcgggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt
      781 tggcagcttt gccgatttca aagctcagtt tactgatgca gcgatcaaaa actttggttc
      841 tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa
      901 cgcgggtact ccactgacca ccgatgcaac tccgctgctg accgttgatg tctgggaaca
      961 cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact tctgggcgct
     1021 ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcaac
     1081 attgcctgat gcgcttcgct taccatacca actcggatga agcgttaaca ctgcatccgg
     1141 caagataaat cgcacgttgc cagcaactgt aacgcagaag gttatccttc tgcgttttta
     1201 tttaattagc tgttggcaac gcaaactgtt tcaggttgtt ttctggctga cataaacacc
     1261 agcaataatg ccagtcccgc gacaatcgcc cccatcaccg gcacaaaact gtatcccagc
     1321 ccagcggaaa ttaccgcacc accagcagct gctcccagag catttccaag attaaaggca
     1381 ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacacgcat cacgcgcatc
     1441 tgtaacggcg gtacgaccgc aaaggttgca gctccccaca ccaccatgct aatagctgcg
     1501 ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcag cagcaacaaa
     1561 aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg
     1621 atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg cgttgcgtgg
     1681 gtaatgcttt gcagtaccgg agagatatag gtgtagaggg taaacattgc accagctccc
     1741 agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt
     1801 ttcacttcag gtcgcgcccc tgcgccacct ttaggtaatg agaagaacag acttaccatt
     1861 gaaatcacgc ccagccccgc cgttgccaga aatgacatcc gccagccgat ggtttcgccc
     1921 aaccaggtcg ctgccggaac gccaccgata tttgccaggg ttaaccccat aaacatagtg
     1981 gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacaac tgaacccaaa
     2041 ccaaaaaatg ctccgtgatt caggctggtc aaaatgcgtg aaagcatgag agtcatataa
     2101 tccggcgcga tggcggaaag tacgttgccg agcgtgaaaa tcgccatcag gaaaatcaac
     2161 gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc caccattacg
     2221 ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc cacaccgcgc
     2281 gcaatgacgg gcaataagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca
     2341 atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca
     2401 ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa atgaaaataa
     2461 caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa
     2521 cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc cacgatcaaa
     2581 ccgaggacga taatcgttgt taccagtgaa aatttaaggt cggtgctcat caagttttct
     2641 ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt gatgaaaaaa
     2701 tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc aagatcggcc
     2761 aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga cgctccgtgt
     2821 cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt
     2881 accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat ggcaacaata
     2941 aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt gatcaacaaa
     3001 acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgatcaa agaattacac
     3061 tactccccta gcgcggtggc gcgtagtctg aaggttaacc acaccaagtc tatcggtttg
     3121 ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcggt tgaaaaaaat
     3181 tgcttccaga aaggttacac cctgattctg ggcaatgcgt ggaacaatct tgagaaacag
     3241 cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct
     3301 gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc aatggtggtg
     3361 atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa cgcgttcgaa
     3421 ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat cggcgttatc
     3481 cctggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat gaaggcgatg
     3541 gaagaagcga tgatcaaggt gccggaaagc tggattgtgc agggtgactt tgaacccgaa
     3601 tctggttatc gcgccatgca gcaaatcctg tcgcagccgc atcgccctac tgccgtcttc
     3661 tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat gggtctgcgt
     3721 gtcccgcagg atgtttcgct gatcgggtat gataacgtgc gcaacgcccg ctattttacg
     3781 ccggcgctga ccacgatcca tcagccaaaa gattcgctgg gcgaaacggc gttcaacatg
     3841 ctgttggatc gtatcgtcaa taaacgcgaa gaaccgcaat ccattgaagt gcatccgcgg
     3901 ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg ttaatcaccc
     3961 gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc actatcgccg
     4021 agatactcaa gcaaccaggt taacgcaggc gacatatcat tttgctgcca tgtcagacaa
     4081 catgccgaat ccgggaaggg gttttccagt tctagtgcta cccacttacc ctcattaagc
     4141 cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga gatacaggtt
     4201 gccgatgatt cccagtcagg cacgacaagt cttttttggt tatccagcaa ccaggtaata
     4261 cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta
     4321 tcatcgctga acgggccatc catcaacgcc agcgggtggt gactggcaac aacgcaactc
     4381 cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt
     4441 gcgccaatcg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc gttgaacact
     4501 tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac gatcatctgc
     4561 cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc gcgccagccg
     4621 ttcgctatct gctgacattg ctgccgggtg atctgcattt ttttgacaac agagcgccct
     4681 tctttgagaa accacgcccc ggcagcggtc agttccacat cacggtgacg tcgttcaaac
     4741 agcggcaccg ccagccactc ttccagctga cgcacggtat agctgaccgc agaaggaacg
     4801 cgatgcaact cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc atcaacaact
     4861 tcgagtgaat attctgacca catagtctgc ctgcaaaatt tttgaaacca gtcatcaaat
     4921 attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt taattgagag
     4981 atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc gggtttgagc
     5041 gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat acaggccgac
     5101 ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc cggttttgcc
     5161 gcagcccagc ttctgtgggg gccgctctcc gaccgttatg gtcgtaaacc ggtattatta
     5221 atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga aaacgccgct
     5281 acgctgctgg tattgcgttt tgtacaggct gtgggtgtct gcgccgcggc ggttatctgg
     5341 caagcgttag tgacggatta ttatccttca cagaaagtta accgtatttt tgcgaccatc
     5401 atgccgctgg tgggtctatc tccggcactg gctcctctgt taggaagctg gctgctggtc
     5461 catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt gctgattctg
     5521 cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga tggtctgacc
     5581 tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata tgcggcctgt
     5641 tcagccagtt tttttgcatg gctgaccggc tcaccgttca tccttagtga aatgggctac
     5701 agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct gattggtggt
     5761 tatggctgtc gcgccgcact gcagaaatgg caaggcaagc agttattacc gtggttgctg
     5821 gtactgtttg ctgtcagcgt cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg
     5881 ctagtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc gatctacccg
     5941 attgttgtcg cccaggcgct gcgtcccttc ccacatgcaa ctggtcgcgc cgcagcgttg
     6001 cagaacactc tacaactggg tctgtgcttc ctcgcaagtc tggtagtttc ctggcttatc
     6061 agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt agtgctggtt
     6121 gctctgggtt acatgatgca acgttgtgaa gaagtagact gccagaatca tggcaatgcc
     6181 gaagtcgctc atagcgaatc acactgatct atatcgatat gcttatactt aggctgctaa
     6241 caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt gttaaagcta
     6301 cgataaatat tatgttttta cggggacagg atcgttcccg actcgctatg gatggtcatt
     6361 tcggcaaggg ttcctccttt ccctctgttc tacgtcggat tatagactcg cggttttttc
     6421 tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga
     6481 ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta ccggatgaca
     6541 actggtaccg tatcgccaac gaattactta gccgtgccgg tatagccatt aacggttctg
     6601 ccccggcgga tattcatgtg aaaaaccccg atttttttaa acgcgttctg caggaaggat
     6661 cgttggggtt aggcgaaagt tatatggatg gctggtggga atgtgaccga ctggatatgt
     6721 tttttagcaa agtcttacgc gcaggtctcg agaaccaact cccccatcat ttcaaagaca
     6781 cgctgcgtat tgccggcgct cgtctcttca atctgcagag taaaaaacgt gcctggatag
     6841 tcggcaaaga gcattacgat ttgggtaatg acttgttcag ccgcatgctt gatcccttca
     6901 tgcaatattc ctgcgcttac tggaaagatg ccgataatct ggaatctgcc cagcaggcga
     6961 agctcaaaat gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta ctggatattg
     7021 gctgcggctg gggcggactg gcgcactaca tggcatctaa ttatgacgta agcgtggttg
     7081 gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga acgctgcgaa ggcctggatg
     7141 tcaccatttt gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt attgtttctg
     7201 tggggatgtt cgagcacgtc ggaccgaaaa attacgatac ctattttgcg gtggtggatc
     7261 gtaatttgaa accggaaggc atattcctgc tccatactat cggttcgaaa aaaaccgatc
     7321 tgaatgttga tccctggatt aataaatata tttttccgaa cggttgcctg ccctctgtac
     7381 gccagattgc tcagtccagc gaaccccact ttgtgatgga agactggcat aacttcggtg
     7441 ctgattacga tactacgttg atggcgtggt atgaacgatt cctcgccgca tggccagaaa
     7501 ttgcggataa ctacagtgaa cgctttaaac ggatgtttac ctattatctg aatgcctgtg
     7561 cgggtgcttt ccgcgcccgt gatattcagc tctggcaggt cgtgttctca cgcggtgttg
     7621 aaaacggcct tcgagtggct cgctaaaggc tattctatcg ccccctctcc gggggcgatt
     7681 tcagatcagg cttctgtacc tggttgattc atggcatttt ctcgtgccgc cagcactcgt
     7741 tctaccgtat ctaccactgc ctgagtttgt ggatcgattt caatgttgac gcgtgcgcca
     7801 agttttttct tcccaagagt cgtgcgttcc agtgtttccg gaattaaatg gacgcaaaaa
     7861 cgcgttggcg tgacttcacc gacggtcagg ctaataccgt cgatgccaat aaatcctttg
     7921 tacagaatat atttcatcaa ctgactatcc tggactttaa accagatctg gcgattattt
     7981 tccgaggtta atattttcgc cacttcagcg gtggtcataa tatgacctga cattaagtgt
     8041 ccgccaattt catcactgaa tttcgccgca cgctcaacgt ttacccaatc ccccactttt
     8101 aaatcgccaa gattggtaat gcgtaacgtt tctttcatca ggtcaaaact gacatggttg
     8161 ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc accggtttcc
     8221 agaccgtcca gcatgtggtc gggtaactcc accacatgtg tgcgaaaatt tggtttctcg
     8281 tcaatcgaca ccagttttgc ggtgccctgt acaatccccg taaacatact tacaactcct
     8341 gaaatcagtt aagacattct gttcagcaca atagcaggtg gaaaacgccc ttaccagtga
     8401 aggggtaaga atgactattt tttcactgga gaattaataa atcctcgcta caatagactg
     8461 aatttcccct gcttcttctt tttgctgccc attcaggcgg ctttttagtc tctcatataa
     8521 ctacaaataa aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct gttattagca
     8581 ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc
     8641 gtgatggcgg gcggctatag tgccaccgac atggcggcgg tcgctatcgg tacttctatc
     8701 tggcttccgg cgatcctctt tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg
     8761 caattaaatg gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca aggtttctgg
     8821 ctggcgggtt ttgtttccgt tctcattatg ctggtgctgt ggaatgcagg ttacattatc
     8881 cgctccatgg aaaacatcga tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg
     8941 ttgttgtggg gcgcgccggg ctatctgttc ttccaggttg cccgtaacca atgtgaaggt
     9001 ctggcaaaaa ccaagccggg tatggtaatg ggctttatcg gcctgctggt gaacattccg
     9061 gtgaactata tctttattta tggtcatttc ggtatgcctg agctcggtgg cgttggttgt
     9121 ggcgtggcta ctgcggcggt gtattgggtc atgttccttg ccatggtttc ttacattaaa
     9181 cgcgcccgct ccatgcgcga tattcgtaac gaaaaaggca ccgcgaagcc cgatcctgcg
     9241 gttatgaaac gactgattca actcggtttg ccaattgcgc tggcgctgtt ctttgaagta
     9301 acgctgtttg ccgtcgtggc tctgttagtg tctccgctcg gtattgttga tgtcgcagga
     9361 catcagattg ccctgaactt tagttcactg atgtttgtgc ttccaatgtc gctggcggca
     9421 gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct caacgctgga tgcgcaaacc
     9481 gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc cattttcacg
     9541 gtttcactgc gggagcaaat cgccctgttg tacaacgaca atcccgaggt tgtaacgctg
     9601 gctgcgcatt tgatgttgct ggcggcggta tatcagattt ctgactctat ccaggtgatt
     9661 ggcagtggga ttttgcgtgg ttataaagat acgcgttcca ttttctatat cacctttacg
     9721 gcttactggg tgctgggctt gccaagcggc tatattctgg cactgaccga tctggtcgtt
     9781 gaacctatgg ggccagcagg cttctggata ggctttatta ttggcctgac gtcggcagcc
     9841 attatgatga tgctgcgtat gcggttcctg caacgtctgc cgtcagccat cattctgcaa
     9901 cgagcagctc gctagtaaag acaaggcgca aacttcacgg gttgcgcctg tgtttttacg
     9961 caggctggag cgttgcgcca atcccgtctt cgtctggctg tagtttcagt gcgttacaca
    10021 gagtttgccg actggcatct gttatctcaa cgtgtatatc accagtaact tcccctttca
    10081 cattgatgaa gtcaggtgaa atttctttcg cactggtttg caggcaaatg gctccctctc
    10141 cacgcaggcg tttcactttc agttgcacat gcgttgccgc accagtgatt agccacagtt
    10201 ttccgccatt tagattgatg ttaccaacat tgaaagtgac actgtcacct tttaccatac
    10261 tgccgtcgag aatcaataca ccgccctgct gtacagtaat cgttgattgt tgcagatctg
    10321 cgccatagcc aataatcatt gaagcgccgt ccagtaatgc aacactaccg gtcagttcaa
    10381 ccggtgcgcg atcttcctct acgacaaaat tttcgtccgc gctggtgggt gacaacagga
    10441 tgactgtccc accttgtatt accgcatttt ccagcgtacc ggcatgcact tcagcccgac
    10501 cacccacatt aacggacagg ttttgcgtct taccggaact gtagagctga agaccatccg
    10561 tccccagacg ataagtggat ccattctcaa caatagtgtc agagtcttca ccaccgagat
    10621 tattcatggt gccaccagca agcaatgtat cactggcttt ccccgccagc ataaaaacgc
    10681 cgccctgatt aatctcacaa ttgctggctt ccccacctgg atacaccaac aattcaccac
    10741 cttcatctac cacgaccgct gaggttgtcc cattgaccaa caggccaccc tcttgatcga
    10801 gaataatttt ttccgcgcga tggccttcca gtacacgcag gttaccgcca ttttccagca
    10861 acaaaccgca ggcataacct ttatcaacgc tgaattcacc ttcgggatgg cggccattaa
    10921 ccgtagcgag cgtagagaga ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa
    10981 tggcaccgtc gctgaccgat agcgtgccat tgttgccgta ctgtgtgcat ttggcgatcc
    11041 caccggttgc cactttcaac cagccagccc ccgtgaccac ggtattttcg gctcttccgc
    11101 cagcttcaac aattaactca ccttcagtaa gttgcgtcgc gccaatgacc tcgccggtct
    11161 taacaatttg cacgttcccg tcgctcatca agtttttcgc atcagatccc atgatttatt
    11221 cctttgctgc atctgtgtgc ctttattgct acctaagtgt aaaggctacg gaggatttat
    11281 ccacgacaga ttttgagatg gtggcaaaca actctgttta aactctgata cacggattat
    11341 tgggttgtat ctgatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    11401 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    11461 gcctcttgcc accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    11521 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    11581 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    11641 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct
    11701 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    11761 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    11821 tggcccattt ggcgacgcaa tggttgagca gcttgaaccg cttgctgagt cgattaatca
    11881 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    11941 gatctacctt tttactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    12001 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    12061 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    12121 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    12181 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    12241 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    12301 atttctatct tcgagcaggc tgatgaagcc ggtgtcggga tgccatacag tgatgaggaa
    12361 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat ttattgtacg
    12421 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    12481 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    12541 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    12601 tcatgccagg ttaccgatct gcaaattata aatgctggcg tcatgctcgc tacaaatcag
    12661 gatttaccca gcgagacgtt tgatttagca gtgatcgcga cgggtcacgt ctggcctgat
    12721 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    12781 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactaga tgcggcaatg
    12841 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt catttttcac
    12901 cgcgataacg caagtgaaaa gctaaatatt acgttaatgt cgcgcacggg tattttaccc
    12961 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac tgatcaggca
    13021 ttaaatgctg agattcaaaa aggcgaagag ggccttttgg atcgggtatt tagattgata
    13081 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    13141 aatgttgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    13201 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    13261 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    13321 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    13381 gcaatccctt cagagtctat tcgtcgccta cttgccttac gtgaagcggg aatcattcat
    13441 attctcgccc tcggtgaaga ctacaaaatg gaaattaatg agtcgcgcac cgtcctgaaa
    13501 acggaagaca acagctactc gtttgacgtt tttattgatg cccgcggaca gcgtccgctt
    13561 aaagtgaaag atattccttt ccctggacta cgcgaacaat tacagaaaac aggggatgaa
    13621 atccctgatg ttggcgaaga ttatacgtta cagcaacccg aagatattcg tggacgcgta
    13681 gcgttcggcg cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca
    13741 tgtgcagaaa ttggtgaggc gatggctcgg gcggtcgtaa agcctgcatc ccgtgctcgt
    13801 cggcgtcttt cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca
    13861 ttaagacgat agcggactcc ggttcgaata ttgttttcac cttcaatggc gacggcgaag
    13921 cgaactcttt cgccgtcatg atatgccagt gcggcgcttc cccctggccc gagaataatt
    13981 gagtcgacca cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca
    14041 ttgtcgccgc tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca
    14101 ccattactgg cgacatgaca tcgttcaccc aatgtacaaa cgcgtactcg cattccagta
    14161 ttcgcaatcc gactactatc tccagcgctg cttatccgcg aagaattacc ggttacagca
    14221 acccgtgcgc gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc
    14281 acgctataac ccatactggc aatttgcgcg gcatacccag cggttgcgat tctggcatta
    14341 tcctcacaac agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatgct
    14401 ctctctccgg gatctgtggc cagtttttgc atagaagaaa cttcctgatg cgcgaagttt
    14461 tcatccgcca gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat
    14521 ccttcctcaa tcagtgcatg atgaatatcg gcataactac cgccgcgtgg gaaatggcgc
    14581 aggaaccagc gatacatcac cgctccgata cgccattctc gcaggcctgc tcgtgtaatg
    14641 atcattcgtt agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta
    14701 cgccccgtgg tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga
    14761 gcaaaatgcg cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc
    14821 ggatagagac acagcagccc cgtcaatagt aacaacggca gcaatccaaa catgacacca
    14881 atataggcaa cctgctgtaa gggattaaat ttagactggg ttgttgccgg gaaaggatgt
    14941 tcttccccct gcataatgcc aaacaaataa aatcgcgttt gttttgccgc tcgctccagc
    15001 caaccctgac gacgaatgcg atagtggtga ccattacccc caacggcatt gatcagaaca
    15061 aagccgagcc agcatgccag taacaaaaat ccgcaaactt catgcaacgc aaccagactt
    15121 ttaaccgcag ttgcgcccac cagcgcaaag tgatttatca gcccacttgc cagcaacaat
    15181 acaaagagta acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaatc
    15241 ttctcaccat gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcatgcagc
    15301 cccagcacca accacatccc aaccagcaat agtccggcaa tgatcaacca caccggccaa
    15361 aattcgggag tgaattgtgg cacatagttc gccaactggc tctgaaactg ttcagcatgt
    15421 tgcgacgggt tcatacattt tcctttttaa tcaaatgttg accgaatcgg cgatacagat
    15481 gcggtttccc ggcaccaggt agctgatatt gatagtattt attttcctgc aaccacgcct
    15541 gaatttctgg gctatcttcg cgaccaaaga tcaatgcatg ttccggacag gcgctgacgc
    15601 aaatgggcgg aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca
    15661 ctttagtgac agggttaagg tagcgaacct ggtaaggaca agcgccaatg caataactgc
    15721 agccgatgca ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgacgccc
    15781 ctgtcgggca tacgtcgatg caaggagcat cttcacagtg ctggcatgac tggcgaaaat
    15841 agtgatactg cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc
    15901 ttccctgagc ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat
    15961 tgcaccgtga ctcgtcatga atcatggcat accgaacttt cttttcttgc ctcgtgttcg
    16021 ctaacagaga agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt
    16081 tgcgtcgagt gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta
    16141 tccatgacat ctccctgaaa tttcaggggt tctgaaactg ctctacctca aaccattcgc
    16201 ataagccata cgccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg
    16261 tcgatgactc cacctgccgt atatcagcat attcaggtgc aatatccatc gcggcgatcg
    16321 cggcttcgat ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt
    16381 aaggacgagg atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt
    16441 cctgctccac aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt
    16501 aatagtaatc atcctggctt cccgtcaaac aagcgatatc cgcgtggtct ccgttctgct
    16561 taatgctttc cagcaaaact gacagttcct cttctgcaac cgaatagggt ggcgagagaa
    16621 acacggcgcg cgccaccagc tgcccagcag cagaatgctg acgaatgaac tctgccagga
    16681 tttcagcagg ttctggttcg gttaccgtat caacggggtg acgcatcccc tgcaaccatt
    16741 gccgcttcgc ctcgtcaatt tcgctgactt ccgctaacgg caattcatcc tgatggttca
    16801 tctcaccatt ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg caatatcttc
    16861 cagccccaga cgctgcaatg tttcacgggt cggacaacca agcagtgggt cccagcccat
    16921 ttctttgtag aacatcgtga gcgaagcatg catatcgtca cggtccattt tgtccgtacc
    16981 ttcagtaaag accgggatct gaggatcctt gtcgaatacc caggaacaga taagatcgtg
    17041 ttcattacgc atatctttgg tttgcatcag ttttaccgtg taggcgcgat gcaacgtaaa
    17101 gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccag tgatcgcttt
    17161 gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca gcggcgaaac
    17221 ggtcattggc cagacccagt tgcacagggt gacggcatta tgcaaacaga cccgcaacag
    17281 cgaccactta gcatatttaa tttttgcgtc attgattggc gtgtagtttt tggtttcatc
    17341 gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt tcaatggcaa
    17401 gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga acatacagtt
    17461 aacaatggaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat agccaaatgg
    17521 cgaccagagt ttatttttcg cgtagcccca gtactcttca cccaaattcc agcgttctgc
    17581 gatggcatat gaaccatcag ccaggtgact cagctcaccc acacgatgcg ccagacggta
    17641 gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc gaatttccgc
    17701 atactcttca gctggtagaa cacgcttgaa cacccctttg ctgtaacaat aagtaaagtc
    17761 gcgatgcagc tgcccatagt tacaccatag gccgtagtca tcgaatagat tcagaccaac
    17821 caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc cgtttgggaa
    17881 gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgccag gcacgccaaa
    17941 ctctttcact cgaggaatat tcatttgggt catacaacgg atcgggcaag aatggcagcc
    18001 gctcattttt actgtgtatt tctctgccgc cggacctaag tcaaaaacgg atttataggt
    18061 acgaaagccg accgtgttct gattgcctgg cggaatttcc cccgtttcaa tcggaccacc
    18121 ttcagccgcg ccccaaaaca gccctttacg tgctgtccaa cgtgacttgg gatctgaata
    18181 ctctgcccac gattgcggcg tacttggcac gacatggttg ttattcgcac cgataagttc
    18241 agtcatcatg taatcgttga gacgcttcat ctcctgacga tcggcaatgt tgaccccttt
    18301 cgtcccctca atcgcaatcg ctttaaggtt tttcgaaccc attattgcgc cagttcctgc
    18361 accaccgctg tggttacggc tattcaacat gccagagaga ggaacaaggt tttccccagc
    18421 ctgaccaata gccgccacac aggtttcagg gctggtcaat cgacaaatct cttccgtcgt
    18481 tgcgcgcgta ccttttcccc ataagaaatc ggctttttcc aggctaactt tgtcatcttt
    18541 aatattcagc cataccggtg atttcgcttt cccttcgata ataatgacgt cgtatccggc
    18601 gaatttcatt tgcgctgcaa aaaagccccc catatgggca tcgacgacta aatttccttt
    18661 ggtaaaagta gaaagtgagg tgatatttac gcgagaactg cagggggcac cagatccagt
    18721 taatgggccg gtagcaaaga ctaatttatt cgcttcatcg aaaggtttcg tgcctggcgg
    18781 tacttcgtca tacataattt tgtagccgaa gcccatacca ccgacaaaac ttttaaactt
    18841 actggaatct tcgagggtaa tatttcctgt cgtaagattg actcttaata tattacctgt
    18901 ccaaccgtta gccatgattt tttcctttgc aagattcaca cagtaatatc tttccactcg
    18961 ataattttta acgcccctgt tgggcaggcg tatgcgcatt caccgcataa cacacatttc
    19021 gaggattttt tactttcggt atttacggtg gccatcatcc acgggcatgc cgtagtacag
    19081 gcgctacagc caatacaacg tttatgatcg acggtaatac agccttcttt ctgctgccag
    19141 gtaatcgcac caatcgggca gacgttcatg cattgcggct ctttgcattg acggcaggtg
    19201 tccgcggtat agttgagatc gccatacagg ccgccgccag agccaacccc gttgtcgcca
    19261 aagaaataat tgcgatggat tttgatacgg gagaagaatg tccctactga accatcgttg
    19321 aagttggtac aagagatttc acagcggtgg caacccgtgc atcgagcacg ttgggtcacc
    19381 agcacccctt tgggtgtatt tatcaaccct actgtgccgc tatcaatatc ttcctgtttg
    19441 caacccaaaa gtgacagtaa cgcaggggca attgttaaac cagcgaggcc ttttcctgat
    19501 atacgcaaga attctaatcg cgttaagcca atatctaata gtggacgatc aaccgggttc
    19561 atttatttcg catcctcttc gcaaacacag tgctgacaat tcagcagact gatattaagc
    19621 attcaactaa tacgctgctg actctgtccc caacaccaga tattttccct aacccgatag
    19681 tgtatgagaa aagatttaac gctgataaac taatccttta tggttaggag aaaa
//
LOCUS       DAYPHN010000085        19732 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000085 DAYPHN010000000
VERSION     DAYPHN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.085"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(46..525)
                     /locus_tag="ACG0BW_002863"
     CDS             complement(46..525)
                     /locus_tag="ACG0BW_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFK7259799.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVMSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLTNARISNIGLTVPHTINDSPGQPEESVSFTYESINWEHIVAGTSAYSLWSERIF"
     gene            complement(543..1901)
                     /locus_tag="ACG0BW_002864"
     CDS             complement(543..1901)
                     /locus_tag="ACG0BW_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFK7259800.1"
                     /translation="MSNYALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKL
                     VESLALSIFKANGVDLHTATYYTLARTRNQGLAGFCEGSELLAAMITHEWDKFWPQGG
                     PARTEMLDWFNTRTGNILRQQVSFSENDLPLLYRTERALQLICDKLQQVELKRQPRVE
                     NLLYFVQNTRKRFEPQPGNRTDTAAQTTVRTLVYAPESTASTTAEAVPPLPDLPEMRV
                     EVRGVANNADNVRQGSTVKGFVAGAACTAVIAAVLWWWQAYPIQRQLTQVRDTAQGAA
                     TAWLASPVLKEYEQYLQQLLDAPPLQPLETGMQMMRTADTLWPESLQQQEASRMWSNT
                     LRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQET
                     PLEYLLTQYQETRARKQDTQALEKEINERLDGLLSRWLLLKNTGQDMATDTRTEPVHP
                     TH"
     gene            complement(1912..5349)
                     /gene="tssM"
                     /locus_tag="ACG0BW_002865"
     CDS             complement(1912..5349)
                     /gene="tssM"
                     /locus_tag="ACG0BW_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFK7259801.1"
                     /translation="MLALAWIFLLVWIWWQGPKWTLYEQHWLAPLTNRWLATAVWGLI
                     ALIWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLRLRRHLDNRRY
                     LWQLPWYMVIGPAGSGKSALLREGFPSDIIYTPESIRGTEYHPLITPRVGNQAVIFDV
                     DGVLTSPGGDDLLHRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLTADKSRRETLV
                     QNLRQQLQEIRQSLHCRLPVYVVLTRLDLLTGFAALFHSLDKKDRDAILGVTFTRRAH
                     ESDDWRSELGAFWQTWVQQVNLALSDLMLAQTGAAPRSAVFSFSRQMQGTGEIVTALL
                     AALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLATWPLVETTPYF
                     TRRLFPEVLLAEPNLAGENSVWLNSSRRRLTAFSACGAALAALLVGSWHHYYNQNWQS
                     GVNVLAQAKAFMDVPPPQGTDEFGNLQLSLLNPVRDATLAYGDYRDRGFLADMGLYQG
                     VRVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNNAPPESEEKLAVLRVLRMMEDKSGRN
                     NEAVKQYMARRWSNEFHGQRDIQAQLMAHLDYALEHTDWHAQRQSGDSDAVSRWTPYD
                     KPVINAQQELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNVFVAGNDEKLV
                     IPQFLTRYGLQSYFVKQREGLVELTALDSWVLNLTQSVAYSEADREEIQRHITEQYIS
                     DYTATWRAGMDNLNVRDYEAMSALTDALEQIISGDQPFQRALTALRDNTHALTLSGKL
                     DDKAREAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQLTELHRYLLAI
                     QNSPVPGKSALKAVQLRLDQYSSDPIFATRQMAKTLPAPLNRWVGKLADQAWHVVMVE
                     AVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKPDGILDNFYKNNL
                     RLFLENDLTFGDDGRVLIREDIRQQLDTAQKIRDIFFSQQNGLGAQFAVETVSLSGNK
                     RRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGRAPHSIAFSGPWA
                     QFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYRVHVDTEDNPFTGGLFSLFRLPDTLY"
     gene            complement(5459..6901)
                     /gene="tssA"
                     /locus_tag="ACG0BW_002866"
     CDS             complement(5459..6901)
                     /gene="tssA"
                     /locus_tag="ACG0BW_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFK7259802.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFVDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATIQRDALLGELAKLAQCWQAHN
                     APELAKATDDLFALFQRAFRDVAPEMPSSARTAAIAPQATAGSVPDMSPHTVAAQPPV
                     AATPIPQVTVDSHDDKAWRDTLLKVAAILCERQPESPQGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YKQVADAIRDEVTGFLARLPALINLLFNDRTPFVSEQTKQWLASSGSVNQTAPVVQTD
                     EELQAARACFDENGLEAALRYLENLPEGDPRHQFHRQFFGAQLLEEAGMVQLAQQQYR
                     ILFRTGLHMMLSEWEPSLLEALEQKFTAEQ"
     gene            complement(6906..7649)
                     /gene="vasI"
                     /locus_tag="ACG0BW_002867"
     CDS             complement(6906..7649)
                     /gene="vasI"
                     /locus_tag="ACG0BW_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFK7259803.1"
                     /translation="MSRLRVFLQTGWFIAGLMAGLPASAAPAETSSMAGVAVAAATAT
                     PSDAAATLQSMRSCRREPAALERLDCYDRILSPEQPAFGGAALVKARYQGEAWTRATE
                     QEKRRQGNTTGLLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGTD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            complement(7646..10378)
                     /gene="tssH"
                     /locus_tag="ACG0BW_002868"
                     /gene_synonym="clpV"
     CDS             complement(7646..10378)
                     /gene="tssH"
                     /locus_tag="ACG0BW_002868"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFK7259804.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHMLLQ
                     MLVIPRSDVRVIAERTGISAEDLRQALTVESYPGGRSAEGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTAINRDQLQQDFTAWTKESAESV
                     DLAGGQAFRATETGDTLLARYAKNMTEDARNGKLDPVLCRDHEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVISSPVPIILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDEALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALATLSQQQETEIRQLERER
                     RIGLRTDTARMTEVLVQYDETLTALDELEAAWHQQQALVQEIITLRQQLLGVAEDTDA
                     VVDTPPESEQDNTGAIPADEAGSVQPEETAETVSPVQRLAQLTAELDALHNDRLLVSP
                     HVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIASLHKHLLTA
                     RADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSR
                     LIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGE
                     GRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFKPALLARMEVVPYL
                     PLSKATLATIIAGKLARLDNVLRSRFGADVIIEPEVTDEIMSRVTRAENGARMLESVI
                     DGDMLPPLSLLLLQKMAANTAIARIRLSVADGAFTADVEDVLNDESVTEDETVL"
     gene            complement(10388..11119)
                     /gene="icmH"
                     /locus_tag="ACG0BW_002869"
     CDS             complement(10388..11119)
                     /gene="icmH"
                     /locus_tag="ACG0BW_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFK7259805.1"
                     /translation="MEPESGTSGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQ
                     AMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAALGNGWSNKNEWI
                     KQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGFRGRYKVAIQQQ
                     DEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIRHVLCGGLVVLAV
                     FYLFYLLRLDSQTQDILHQLNKLLR"
     gene            complement(11166..12512)
                     /gene="tssK"
                     /locus_tag="ACG0BW_002870"
     CDS             complement(11166..12512)
                     /gene="tssK"
                     /locus_tag="ACG0BW_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFK7259806.1"
                     /translation="MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLNRRLDALGDFFW
                     GFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIY
                     LALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
                     ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRA
                     ADLAGRIGSPEQSGIADVAEFMMLQMLNRHQMQFTHRSQLHTLHPEAFYQDLVGLLGE
                     LMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTW
                     IATISDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLH
                     TLTAAPRQLPWHEGYVYFELEKGTPAWQDVIKAGALALHISGTFPDLNLQLWAIRG"
     gene            complement(12515..13039)
                     /gene="tssJ"
                     /locus_tag="ACG0BW_002871"
     CDS             complement(12515..13039)
                     /gene="tssJ"
                     /locus_tag="ACG0BW_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFK7259807.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFQAADYDQIATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            complement(13036..14316)
                     /gene="tagH"
                     /locus_tag="ACG0BW_002872"
     CDS             complement(13036..14316)
                     /gene="tagH"
                     /locus_tag="ACG0BW_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001511117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFK7259808.1"
                     /translation="MAEEKQQSRQASLTLQVMNGNELESGRAAKCLFSENGGDIGHTP
                     ECHWQVQDRAGSIAARACTVIRHDGAYCLRCLTPGLMINLAPVSSDALIRLRQGDEIQ
                     LGALALKVFLHDGVAVTYDERMATPETIVMNRDSLADTLLTTEGQPAYPGMPFQHQLA
                     PTVAHGFSSDPLQALQTESLMVADDPIAPRVSRPVAPASDLTGANGINTPFMDLPPGN
                     ARHEDGDEFSAMAQYHLAVTPLLRGMECPLTLHNSQDADEFLEEAGRALQAAIKGLLS
                     LQQQQNSLSDKHLRPLEDNPLRLDMDYPTALNVMFAEGKSPVHLAAPAAIAESLRNIR
                     HHEEANRAAIVEALRVMLDAFSPGNLMRRFAQYRRSHELRQKMDNAWAWQMYSNYYDE
                     LASSRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            complement(14333..15382)
                     /gene="tssG"
                     /locus_tag="ACG0BW_002873"
     CDS             complement(14333..15382)
                     /gene="tssG"
                     /locus_tag="ACG0BW_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFK7259809.1"
                     /translation="MAGENGSARPYIATKPPLSADVRNCNFYVLMEALYRRYGAPGQD
                     ISLRTEPAQEIVRFSSDANIGFPGTDLSVLSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRNGGTDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLGDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            complement(15346..17187)
                     /gene="tssF"
                     /locus_tag="ACG0BW_002874"
     CDS             complement(15346..17187)
                     /gene="tssF"
                     /locus_tag="ACG0BW_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFK7259810.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSEKSALAAQKVLSDNHDKIVSSVACHFTLARDIWLQPLRILDTHNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLEAAGFENQDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPASAFTLRLHFNRPLPADIKLRRDSLRLHCTPAINLFTHYAEPVRPDGRIAEYPL
                     RASHRHPDAYDIFQISTVTSKVKVSGTESNPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLFHRGLEHSIAFVHADGSIPDSSRFNDDVITTSLICTNRMIPARLHIGDI
                     CVAVNKNPAVASFHNVTRPTQPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQEAWEWQEKMGQHALI"
     gene            complement(17193..17618)
                     /gene="tssE"
                     /locus_tag="ACG0BW_002875"
     CDS             complement(17193..17618)
                     /gene="tssE"
                     /locus_tag="ACG0BW_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFK7259811.1"
                     /translation="MQRRDSGPTSSLFERIREAANPPSYQNTKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELRFHIVATVDVSETRGVFEFDILLDNHQRYCVE"
     gene            complement(17623..19107)
                     /gene="tssC"
                     /locus_tag="ACG0BW_002876"
     CDS             complement(17623..19107)
                     /gene="tssC"
                     /locus_tag="ACG0BW_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFK7259812.1"
                     /translation="MSVKEDIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPARTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKKFPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYVADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            complement(19130..19633)
                     /gene="tssB"
                     /locus_tag="ACG0BW_002877"
     CDS             complement(19130..19633)
                     /gene="tssB"
                     /locus_tag="ACG0BW_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFK7259813.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDSREQLIQE
                     LGIAAQK"
BASE COUNT         4090 a         5500 c         5579 g         4563 t
ORIGIN      
        1 tttttaaaat ataacaataa tgcagatgaa agactcccag gtaacttaga aaatccgttc
       61 actccacaga ctgtacgcac tggttccggc gacgatgtgc tcccagttga tgctctcata
      121 ggtaaatgac acagactctt ccggctgccc tgggctgtcg ttaatggtat ggggaacggt
      181 aaggccgatg tttgagatcc gggcattagt cagtttcagc ttgtagtacg gctcattaat
      241 accaaaacgg ttggtgcgat agaagacaaa atcacaggtc agacattcat tctccgaaat
      301 ggccttgccc agaagggggg aggatttgtc caccggtttc gtgatagtca gttcctggtg
      361 attgacgttc tgtgcgcgac tcagcccatg ggacagtgac atcaccatga tctgatcctg
      421 atgtgcaatc tgggccttgt taccgatgga cggcattgac gagcatccgg atgaaatcag
      481 tccctggagt tctccgttta gtgtcagata gacgggattt gccattgtta ttctccttaa
      541 cctcaatgag tcggatggac aggttcggtt ctggtatccg ttgccatatc ctgtccggta
      601 tttttcagca gtagccagcg gcttaacagc ccgtccagcc gttcgttgat ctctttttcc
      661 agcgcctgtg tgtcctgctt ccgtgcgcgt gtctcctggt actgtgtcag caggtactcc
      721 agcggtgttt cctgattcag taaccgttcg gcctgccagg tcaccgtctt gatatacgag
      781 agggtgaaac cctgtttctc cgtctccttt ttcatcatca gatccgcaaa gtcccgcaaa
      841 ttctgccgcg cctgttgcca gcccttcatc tgcggactgg cttgcgcccg gtttctcagc
      901 gtgttgctcc acatcctgct ggcttcctgc tgttgcaggc tttccggcca gagggtgtct
      961 gcggtgcgca tcatctgcat tccggtttcc agcggctgta gtggcggggc atccagaagc
     1021 tgttgcaggt actgttcata ttctttcagt accggggagg ccaaccaggc ggtggcggct
     1081 ccctgtgcgg tatccctgac ttgcgtaagc tgccgttgta tcggatacgc ctgccaccac
     1141 cagagtactg ccgcgataac ggcagtacag gcggcaccgg caacaaaacc ttttaccgtg
     1201 ctgccctgtc taacgttgtc ggcgttattt gccacaccgc gcacctccac ccgcatttcc
     1261 ggcaggtcgg gcaggggagg cacggcttcc gctgtggtgg aagccgtgct ttccggcgca
     1321 tagaccagcg tccggaccgt ggtctgtgcc gccgtatcag tacggtttcc gggctgcggc
     1381 tcaaaccgct tgcgtgtgtt ctgcacaaaa taaagcaggt tttccacgcg cggctggcgc
     1441 ttcagttcca cctgctggag tttgtcgcaa ataagctgta aagcccgttc cgtgcggtag
     1501 agcaacggca gatcattttc ggaaaaggac acctgctggc gcagaatatt cccggtgcgg
     1561 gtattgaacc agtccagcat ctcagtccgc gccgggccac cctgcggcca gaatttatcc
     1621 cattcgtggg tgatcatcgc agccagcagt tccgagcctt cgcagaaccc tgccagcccc
     1681 tgattacggg tacgcgccag cgtatagtag gtggcggtat gcaaatccac cccgttggcc
     1741 ttaaaaatcg acagcgccag cgattccacc agtttccagt tcagctccgg ctgtgacggg
     1801 tggttagctt tgttaatttc ttcgcggatg gcgctgaact ccggcagggc gcgcggatcg
     1861 ctgccggtaa cgatagtttg tgtcagtgcg taattgctca tcactatccc cttaatacaa
     1921 cgtatccggt aaacggaaca ggctgaacag accgccggtg aacgggttat cttcggtatc
     1981 cacatgcacc cggtaaacca ttgcgccgcc gtccacgtta aagcgcacgt taaaggtgtc
     2041 actggtcaca ttggtcaact ggcccgcgcc gaacaggcgg aactgcgccc acggtccact
     2101 gaacgcgata ctgtgcggtg ctctgccgct ggtgccaatc agcgtcagct tgctttcatt
     2161 gccttcacgc atgttgttcg gccagaccag atgggcggtg tagttgcgtc cctggctgta
     2221 gtccactaac tggccgtcca ggttaagtac gctgcgccgc ttattgccgg aaagcgatac
     2281 ggtttccacg gcaaactgtg cgcccagccc gttctgctgg ctgaagaaga tgtcgcggat
     2341 tttctgcgcg gtatccagtt gctgccggat atcttcacgg attaacactc tgccgtcgtc
     2401 gccaaaggtc agatcgtttt caaggaacag gcgcaggttg ttcttgtaga aattgtccag
     2461 aatgccatcc ggtttaaaga aacgttcaaa cgaatccagt gaggcatcct gtgtggcgcg
     2521 cggattaaac ggatagttat cggcaagctg ctcgttgaag ggtttcacta cattgtcgcg
     2581 ccagtccact tccatgtaac gaacggcttc caccatcacc acatgccagg cctgatccgc
     2641 gagcttacct acccagcggt taagcggtgc agggagagtt tttgccatct ggcgggtagc
     2701 gaagattgga tcgctgctgt attgatccag acgtagctgt actgctttca gcgccgattt
     2761 ccccggcact ggcgagttct ggatcgccag caggtaacgg tgcagctcgg tcagttgctg
     2821 gtacacggcc tgtagcgtac tcgccttgtc cttttgctcc tccagtgcgc tgttttccgg
     2881 tgcgaactca tgccccagcc gggataacag gcggtaatcc atctcattta ttgccgcttc
     2941 cctcgcctta tcatccagtt tgccggagag cgtcagcgcg tgggtattat cgcgcagcgc
     3001 cgtcagcgca cgctggaatg gctgatcgcc gctgataatc tgctccagcg cgtcggtcag
     3061 cgccgacatg gcctcatagt cacggacgtt gaggttatcc attccggcac gccaggtggc
     3121 ggtatagtca ctgatgtact gttcggtgat atggcgctgg atctcttcac ggtcggcctc
     3181 gctgtaggcg acgctttgcg tcaggttcag tacccacgaa tccagcgcgg tcagctcaac
     3241 gaggccctca cgctgtttga caaaatagct ttgcagtcca tagcgggtga ggaactgcgg
     3301 gatcaccagt ttttcatcat taccggcgac gaacacgttg tcgaaggtgg gaccaacctg
     3361 gtcgcgcaaa ttcaaatcgg cgggcaacac gcttaatgct ttggtgcgca gggtctggta
     3421 gacacgctgg tatatgggca gcttgctcag ttcctgctgc gcattaatga ccggtttatc
     3481 atagggggtc cagcggctga cagcatcgct gtcaccgctc tggcgctgcg cgtgccagtc
     3541 ggtgtgctcc agcgcatagt ccagatgcgc catcagttgc gcctgaatat cgcgctggcc
     3601 gtgaaattca ttgctccagc gccgcgccat gtactgtttt accgcctcgt tgttgcgccc
     3661 acttttgtct tccatcatgc gcagtacgcg cagcacggcg agcttttctt cgctctctgg
     3721 cggggcattg tttagatccc ggatcaggcc gttcattaac gaggggagat aacgctgctc
     3781 aagaagctga atgtaggttt gctccacata cggccctacg cggacgccct ggtacaatcc
     3841 catatccgcc agaaaaccgc gatcgcggta atcgccatag gccagggtgg catcgcgtac
     3901 cggattaagc aacgacagtt gcagattgcc gaattcatcc gttccttgcg gtggtggtac
     3961 gtccataaag gctttagcct gtgccagtac gttaacgccg gactgccagt tctgattgta
     4021 ataatggtgc cagcttccga ccagcaatgc cgccagcgcc gcgccacagg cggaaaaggc
     4081 ggtcagcctg cgccgggagc tgttcagcca gacgctgttt tcacccgcca ggttcggctc
     4141 agccagcagg acttcaggga agaggcggcg agtaaaatac ggcgtcgtct ccaccagagg
     4201 ccaggttgcc agcgagctgt tacccagccc gtactggcgg gcggcggact gcgtgaaaat
     4261 atcatccacc tggccacgct gtagcgatga tgtgagccag acgccacgca gcattacatc
     4321 catgttctca ccgtccagca atgcggcgag cagtgcggtg acgatttctc ctgttccctg
     4381 catctgacgg gagaagctga acacggcgct gcggggagca gcaccggttt gtgcgagcat
     4441 cagatccgac agcgccaggt tcacctgttg tacccacgtc tgccagaaag cccccagttc
     4501 gctgcgccag tcgtcacttt catgggcgcg gcgggtaaac gtgacgccga ggatcgcatc
     4561 gcggtctttt ttatccagtg aatggaacag cgcggcaaag ccggtcagca gatccagccg
     4621 tgtcagcacc acgtaaacgg gcagacggca gtgcagactc tggcggatct cctgaagttg
     4681 ctggcgcaaa ttttgtacca gtgtctcacg gcgggattta tccgccgtca gcagatcggg
     4741 aagatcgagc gtcaggatca ggccgttgag cggctggcgc gcgcgcgttt gcatcagcca
     4801 gcccagccag tgttcgcgca ggcggcggtg gagcagatca tccccgccgg gcgaggtcag
     4861 tacgccgtca acatcgaaaa tcaccgcttg gttgcccact cgcggtgtga tcagcggatg
     4921 gtattccgta ccccggatgc tttccggcgt gtaaataatg tcagatggaa agccctcgcg
     4981 cagcagagcg cttttaccgc tacccgcagg accaatgacc atataccacg gcaactgcca
     5041 cagataacgg cggttatcca gatggcggcg caggcgcagc agccagtgat ccagatattg
     5101 ctgctggcgg tggagttcca cggtcaacgg atctttttct tcctcccgct gctgtttctg
     5161 ctgtttttcc agcttttgca gacgcttcat tacccgccag gtgagccaga tcagagcgat
     5221 aagcccccag acggcggtcg ccagccagcg gtttgttaga ggagccagcc agtgctgctc
     5281 atagagcgtc catttcggac cttgccacca gatccacacc agcagaaaaa tccatgccag
     5341 cgccagcagc caggcggcgg ataccctgaa tcgcggcatc gctggcctga gagcagatct
     5401 cagcgtgctg aacagtcggg atgtcggaaa tctgaacacg gtaactcctt gttatctttt
     5461 actgttctgc cgtgaatttt tgttccagtg cttccagcag ggacggttcc cattcggaca
     5521 gcatcatgtg taatcctgtt ctgaagagta tccggtactg ctgttgtgca agctgaacca
     5581 taccggcctc ttccagtaac tgcgcgccga aaaactggcg gtgaaactgg tggcggggat
     5641 caccttcggg caggttttcc aggtaccgca gcgcggcttc cagcccgttt tcatcgaagc
     5701 acgccctggc cgcctgtaat tcttcatcgg tctgtacaac cggggcggtc tggttgactg
     5761 agccggatga tgccagccac tgtttcgtct gttcggacac aaacggagtg cggtcgttaa
     5821 acaacaggtt gatcagtgcg ggcaggcggg ccagaaaacc ggtaacttca tcacggatag
     5881 catccgccac ctgtttataa ccgaggcgca gcgcggtctg tgctgagagg taatgaccgt
     5941 ccagccagta cggggccagc agcaggcttt tttccacctg ctgccacagc gccatgtcgg
     6001 cattgttcag ccgcgccagg taatcatcca tcatatcgac agggaaggct gccagtgggg
     6061 tccggccatc gctttctgcc tgcggggtac tggtgatggt ctgccacaga gcatggcggc
     6121 gcagtcggta accctgcggt gactccggct ggcgctcaca gagaatggcg gccaccttca
     6181 gcagggtgtc gcgccatgct ttatcgtcgt ggctgtcaac agtgacctgc gggattggag
     6241 tggctgcaac aggaggctga gcggcaaccg tgtgcggtga catatccggt acagatccgg
     6301 ctgtggcctg cggcgcaatg gcggcggttc gtgcagatga tggcatttca ggcgctacat
     6361 cacgaaacgc gcgctgaaac agggcaaata aatcgtctgt cgccttagcc agctcaggag
     6421 cgttgtgcgc ctgccagcac tgtgccagtt ttgccagctc tcccagcaaa gcatcgcgct
     6481 gtattgtcgc agcattcccg gcaaagtcct gaacggcaga ctcaaagcgt tttatcacct
     6541 gggcggcaaa gcgttttttg tgggccatgt tctgcggtgc ggcgcatgtc cagtagtgtt
     6601 ctgtatattg tgccagcatc ctggaggcca gcagaatatc accggcatgt tgcagggtac
     6661 gcagcaggtg ggccagcagg cgaaaatctt tggtttctga ggccagaagc atcaggcccc
     6721 ggcgctgaag ttccgggata tcaagctggc tgtgcgccag tgaaccgagc ttgacgattt
     6781 cgctgtcgac aaactcccag tctgcattgc catcatcaag cgcggcggac agcttatctt
     6841 ccggcaggtt ttccagcagg tgtgcaagcc atacatcggg attacgtaag tccattgcca
     6901 tattcttatt ctcccgccca gtggcaggcg tcgcgtagcg gcgcaataac ccgtgccagc
     6961 ccgtcaatgt taaaggtcag ctttccggcg gcgttgtctg tgccggtatc caccgtcagc
     7021 gtttttgcgc cgaacagttg cttaatttca tcgataccgg ataacccccg gctggactcc
     7081 agcagggtgc cgttctcgcg cacaaaccag tggctgcgta atgcccggct gtcggcattc
     7141 agggtgacgg cgatatcatg gacatccaga gggtgcatca gcgccacctg catccgggtg
     7201 atattgtcca cgcaactgaa catcagcacc gggcgcggcg gaacatggcc gatagccggg
     7261 gtggttatca ctaccgtcgg gcgttcaccc ggcacctgcg tcaccagcag ccctgtggta
     7321 ttgccctggc ggcgtttttc ctgctctgtg gcgcgggtcc aggcttcgcc ctggtaacgg
     7381 gctttcacca gtgcagcccc accaaaggct ggctgctctg gcgaaagaat gcggtcataa
     7441 cagtcaagcc gctccagcgc cgcaggctcc cgccgacagg accgcatgga ctgtaacgtg
     7501 gctgccgcat ccgaaggtgt cgctgttgca gcggctaccg ccacaccggc cattgacgat
     7561 gtttctgcgg gcgcggcact ggcgggtaag cccgccatca ggcccgcgat aaaccatcct
     7621 gtctgcagga aaacgcgcaa cctgctcata aaaccgtttc atcctctgtg acggactcgt
     7681 cgttcagaac atcttccacg tctgctgtga atgcaccgtc tgccaccgac aggcgaatcc
     7741 gggcaatcgc cgtgttagcc gccattttct gcaacagcag cagcgagagc ggcggcagca
     7801 tgtcgccgtc aatgaccgat tcgagcatcc tcgcgccgtt ttccgcgcgg gtgacgcggc
     7861 tcatgatttc gtccgtcact tccggttcaa taatcacgtc cgcaccaaag cggctgcgca
     7921 gcacgttatc caggcgggcc agtttcccgg caataatggt agcgagcgtc gctttcgaca
     7981 gcggcaggta tggcaccact tccatacgcg ccagcagggc gggtttaaag aagtctgcca
     8041 gcaccggata cagggcttcc tgcatggttt ccgggtcatc ggcgtgttcg acaatcacct
     8101 gatagccgag gttggaggtg aggaagaaga cgatgttttt acagtcaatc aggcggccct
     8161 cgccgtccgc catctcgccc ttgtcaaacg cctggtagaa caggttgagc acatccgggt
     8221 gcgctttttc cacttcatcg agaagtacca ccgagtaggg tttctggcgg atggcctcag
     8281 tcagcacgcc gccttcgcca tagccgacat agcccggagg ggaaccaatc agccgcgaga
     8341 cggtgtgctt ctcctggaac tcggacatat tgatggtggt caggtactgg cgaccgccgt
     8401 agagcagctc tgccagttgc aggacggttt cggttttacc cacgccactg ggaccagcca
     8461 gcagaaacgc gccaagcggg cgtcccggac ggcgcaggtc ggcgcgtgcg gtcagcaggt
     8521 gtttatgcag gctggcaatc gccaggtcct ggcctttgat ggtgtcacca agccacttcg
     8581 gcaggtcggt gatgaccgac atttcattct gcgacaggcg gttaagcgga acaccagtcc
     8641 attcggcaat taccgccgca atctgttttt tatcgacatg cggagagacc agcaaccgat
     8701 cgttatgcag ggcgtccagt tcggcagtaa gctgtgccag ccgctgtacc ggggaaactg
     8761 tttcagcggt ctcttccggc tgtacgctgc cagcttcatc agccggtatg gcaccagtat
     8821 tatcctgttc tgactctggc ggcgtatcca ctacggcatc tgtgtcctct gcaacgccca
     8881 gtaactgctg gcgtagcgta ataatctcct gtaccagcgc ctgttgctgg tgccaggcgg
     8941 cttccagttc atccagcgcc gtcagtgttt catcgtactg caccagcact tccgtcatcc
     9001 gggcggtatc ggtacgcagc ccgatacgcc tttcgcgctc aagctggcgg atttccgtct
     9061 cctgctgctg gcttaacgtg gccagcgccg aaatctgttt cggcggcgaa gaaaggttga
     9121 ttgccacgcg ggcgcaggcg gtgtccagca catcaatcgc tttgtccggc aactgacgtc
     9181 cggagagata acgctcgctc agtgttgctg cggcttgtaa ggcctcatcg tcaatcagca
     9241 caccgtgaga ctgttcatag accgccgaca ggccacgcag aataatcgtc gcttcggcag
     9301 cgttaggttc gctgacctta actaactgga agcggcgcga taaggcagcg tctttttcga
     9361 agtatttttt gtattcgctc caggttgtgg cggcgatggt tttcagttcg ccacgcgcca
     9421 gcgccggttt gagcaggttg gagatatcca gcccgccctg ctggttgccc gcgccaatca
     9481 gggtatgcgc ttcgtcgata aacagaatga tcggcaccgg ggaggaaatg acctccgcca
     9541 tcagcccttt gaaacgtttt tcgaattcgc ccttcaccga cgctccggcc tgcaatgcgc
     9601 ccaggtcaag ggtcatgata tcggtgtttt tcagcttgtc cggcacctgg cctgccacga
     9661 tgcgcagcgc cagcccttca atcagtgcgc ttttgcccac gcccgcttcg cccactacca
     9721 ccgggttatt tttacgacgg cggcagagaa tgtcgatcat caaatcgatt tcgtggtcgc
     9781 ggcacagtac cggatcgagt tttccgttac gggcgtcttc ggtcatattt ttggcatagc
     9841 gggcaagcag ggtgtcgccg gtttctgttg cacggaacgc ttgcccacca gccagatcca
     9901 cggattcggc ggattctttt gtccatgcgg tgaaatcctg ctgtaactgg tcacggttta
     9961 tggccgtcag cagacgggca gcagcgggtg gaatatagcg cagcggtgaa tgcagcagag
    10021 taagcagcag gacgccgctg cgcagttcgc tgtgctgcat ctgggctgag gccagcagcc
    10081 atgactcctt gagccattcg atcagcatcg gggagaagct gggatagcct tcggcggaac
    10141 gtccgccggg ataactctcc acggtcagcg cctggcgtaa atcctccgca ctgatgcctg
    10201 tgcgttcggc aatcaccctt acgtcgctgc gcgggataac cagcatctgc aacagcatat
    10261 gggcaacggt gatctcactg gcctgctggc tcatacactc cgaggcagcc atttccagcg
    10321 cctgttttgc aaaggggtta agccgcttta ccagcgtggc taaatcaatc tggatcataa
    10381 gatgtcctta tcggagtaat ttattgagct gatgcagaat atcctgtgtc tggctgtcca
    10441 gacgcagcag ataaaacaga taaaatacgg ccagcacaac aagaccgccg cacagtacat
    10501 gcctgatggt cagccgccgg attaactgat aacgcccgcc ctgtatgttg ttcttctggt
    10561 gaagcagcgg aaatggcgcg tcaccgcgca gtttgtgcag ggcatggtgc aggcgctggt
    10621 agatccgctc aaattcatcc tgttgctgaa tggcaacctt gtagcgcccg cgaaagccca
    10681 gcgaaaagca gaggtataaa aattccagca gatcctggta acgtaccggc tcccggatca
    10741 ggcgttcgag caggataaac actttctcgc cgccccaggc ttcgttatgg aaatgaacca
    10801 gtagcgactg ttttatccat tcgtttttgt tggaccagcc gttgcccagc gccgcttcgt
    10861 caataaacgt gcagagaatg tagcggaacg aaacaatgac gcccggctcg taaccctgct
    10921 cctggagtag ttgttccaca gcctggacat cggttaccac ctgggcaaac agatgttccg
    10981 gcatggcctg gctgttcatg gtggacagac gcatcaccat gccgagcaac ggcgtggcgg
    11041 cgtcaatcat tgggttcagg ctgttgccgc gaagggcgag acggtactga cgtcctgatg
    11101 ttccgctctc cggctccatg ggaaaaaccg acggttcacc catatcgctg atatcgctca
    11161 ttcacttatc cccttatcgc ccagagctga aggttcagat cggggaaggt gccggagata
    11221 tgcagcgcca gcgcgcccgc ttttatcacg tcctgccagg ctggggtgcc tttttccagt
    11281 tcgaagtaaa cataaccttc atgccagggc aactgacgcg gcgcagcggt aagagtgtgc
    11341 agcggaatac ccggcacctg cacgctgacc acgctgcgga ttttgtcggt agcggccact
    11401 tttgactgct ggatgaactg gcgttgtacc tggtcgtagg gcatcctggc gcgcactgcc
    11461 agtaccagct tgcaggactg caacagtgtg ggatcgctga tggtggcgat ccaggtcccc
    11521 tctgaaaaga acagcggcag attctgcgca cgcggcacca gtacggtatt gagtgcgcgc
    11581 cgcagttccg ggatcacggt tctggtgaag gtcgcggtaa gatcccggtg attatacggc
    11641 tccacggtgc agggcaggcg attaccttcg gtaaaggtca tcagttctcc cagcagcccc
    11701 accagatctt ggtagaaggc ttccggatgc agcgtgtgca gttgtgaacg gtgcgtgaac
    11761 tgcatttgat ggcggttaag catctgcaac atcataaatt cggccacatc ggcgatcccg
    11821 ctctgttcag gcgaaccgat gcgcccggcc aggtcggcgg cgcgggtggc aagcagcccc
    11881 tgcacctcgc cggagaatgc cccgagcaac ccgctgacac ggacggcctg aatggtggga
    11941 ataaaggtgt tgtccagcac cagtgcgccg gtagcatttt tattgaggat acggcacagg
    12001 gggagggtca ccatcgcgct gagatcatct gcgccgctga ctatcctggg gatcaactgc
    12061 cccagaccga ccggctgttc gtcgccttcg tcagtgtgaa aatcacggac gcgggtatgg
    12121 gtgaggcggt aacgctccgt accctgaccg gcgctgtgca acccctggat ttcattgatc
    12181 acgtcgctga ttacaggcag cgccagataa atattgtggc tctctttcga tgaaagggtt
    12241 cccggctgga aaggttccgg cagcagatcc tggtcgggaa tgctgaatac ggtgccgtca
    12301 ggcatacagc ccgccgcacg gtcaatcatg attttgccct gggcaagcag ttcggtattg
    12361 atggagagtt ccgtaaagcc ccagaaaaaa tcacccagtg catccagacg acggttcagc
    12421 aggtactcgc aatgacgctg ctgttgctga aaatgctgcg gcagggcaaa cagcccttcc
    12481 tgccagatca ccttgttttt cattgttgcc atcgtcagtt ttcctctttt ttcatctcaa
    12541 tcgcactggc gcggatatgc accagcaggc gatagtggtg gccgatactt tctaccggct
    12601 caatttgttt ccattctgtg gcctggtcgt ctgaaaaata ggcaatgaca ccgatataac
    12661 cggttttctc atccagcttg atcggcggca gtgtttttac ggtgtccggc aacaggttga
    12721 agtcctggtg atcgatatag ttcttcccca gcacgtcggg cagcgcggtg gtggcaatct
    12781 gatcgtaatc ggcggcctgg aatttggagt cgtcgctcag atacaccaac tggacatcca
    12841 ccggcgcggc ttcaccgtcc gccgtcaggt tgctgtccgg ctcggtcagc agcgtgacgg
    12901 tcagctcggt tgactgatcc atcagctttc cgacctgaat atccggattg cggatcacct
    12961 ggctgatttt tttcgctgtt tcacagccag ttaacacaac gctcatcaga acgcaggcca
    13021 gaaacgcttt gccgttcatg cttccggctc ccgctgtttt tcgcgcagca cccggtcata
    13081 gacctgggca taaacttcat tgaacagcat ttcaaatccc tgctgtcggc tggaggccag
    13141 ttcatcgtaa tagttgctgt acatctgcca tgcccaggcg ttgtccattt tctggcgcag
    13201 ctcatggctg cggcggtatt gcgcaaagcg gcgcatcagg ttgcccggcg agaaggcgtc
    13261 cagcatgaca cgcaacgctt ccacaatcgc cgcccggttg gcctcttcat gatgccggat
    13321 gttgcgcagg ctttcggcga tggccgcagg tgcggcgaga tgtaccgggc ttttaccctc
    13381 ggcgaacatg acgttcagcg cggtgggata gtccatatca aggcgcaacg ggttgtcttc
    13441 cagtggacgc aggtgtttat ccgacaggct gttctgttgt tgttgcagac tcagcaatcc
    13501 cttaatggcg gcctgcaacg cccgtccggc ctcttcgaga aactcatccg catcctgaga
    13561 attgtgcagc gtcagcggac actccatacc gcgcagcagc ggggttacgg ccaggtggta
    13621 ctgcgccatg gcagaaaatt catcgccgtc ctcatgacgg gcattgccgg gcggcagatc
    13681 cataaacggc gtgttgatcc cgtttgcacc tgtcagatcg gaggcggggg caacgggccg
    13741 ggaaacgcgt ggggcgatcg gatcatctgc caccatcagg ctttccgtct ggagtgcctg
    13801 gagcggatcg ctggaaaaac cgtgcgcaac cgtgggggca agctgatgct ggaacggcat
    13861 tcccggatac gccggttgtc cctcggtggt caacagggtg tccgccagac tgtcgcggtt
    13921 cattacaatc gtttccgggg tcgccatccg ctcgtcatag gtaacagcta caccgtcatg
    13981 cagaaacact ttcagcgcca gtgcgccgag ctggatctca tccccctggc gcaggcggat
    14041 gagcgcatcg gaagagaccg gggccagatt aatcatcagt cccggcgtca ggcaccgcag
    14101 gcaataggct ccgtcgtgcc ggataacggt acaggcacgc gcagcaatgc ttcccgcccg
    14161 gtcctgtacc tgccagtgac attccggcgt atggccaata tcaccgccgt tttcgctgaa
    14221 caggcatttt gcggcgcgtc cgctttccag ttcgttgcca ttcatcacct gtaaagtcag
    14281 tgaggcctgg cgggattgct gtttttcctc agccataagt ggttccatag aattattccc
    14341 tgattcggat tgtgatggcg ggcggtactt tcggctgacc aataaagctg gttctgccga
    14401 ggcgggagct ggcaggatcg ccaaggcgca tccctttagc ctgctgtggt gctaggcaca
    14461 gccgtaaatc ccaggcaaac tggtcgcgca gaataaagga aaggaacatg gtcagcggca
    14521 ggtgattttc gccatcgggt aaaaatgaga gataacgctc ccgcgacagg ccgccaatct
    14581 gcaacaggaa ttttccgctg cgatccggca cccgtgcgcc gagggtaaaa ttaacgccga
    14641 gtatggagcg tcccggaata tgtccagccg tttttccttt cggattacgt acacccagcc
    14701 ggttctggtg tgccggatca atggcgactt tacgcagttg ccagcgttca atggtgacat
    14761 ccggcaaatc aaaacagtgc gacaccagat tacagataac ctccggcgcg cggcctggtg
    14821 ttgccagaat tccggcgtag gccagcattt tgctgtggtt aatggcaagc ctgtcacgca
    14881 cgctctgatt acccagcccg accagtgcgt acatgcgctg tgaaaaggtg tcagtaccgc
    14941 cgttacgaaa gcagatgtag tagcggtatt tgcgccaggc atgataggca aactgtgtcc
    15001 agcggtggct gaatagatcg agaaattctt tcaggccgtc ttcgttctgt gcggactcct
    15061 gcgccatctg atccagataa tagcccggta gcggcgactg gctgccggaa aagcccagga
    15121 aacgggtttg cagtacatac tgtccgttct ggctacggga aagcacactc aggtccgtac
    15181 ccggaaaacc gatattggcg tcagagctga aacgcacgat ctcttgggct ggctcggtac
    15241 gcagggaaat atcctggccc ggagcgccat accgacggta cagcgcctcc atcagcacat
    15301 agaaattgca gttgcgcaca tccgccgata acggcggttt tgtcgctata taagggcgtg
    15361 ctgacccatt ttctcctgcc attcccaggc ctcctgactt tccgtgttga tgattttcag
    15421 acagtggtac gaattgatgc tggcgtagag agcaaaaaag tgcgagagca ctgtaccaag
    15481 aagataaatt tcaccttcgc agataaatgg atcgggacgt atccacagcg tggtcgccag
    15541 cccgcgaacc ggcacgcctt taaacaacct gtcaacaggt ttggtttcga gtttttctat
    15601 ggcatccagt ttctggcgcg acagtctggc ccgctgcgga tggtgagcgc ctggcaggtc
    15661 gaaggtccgc aggatctgga tcagtgtctc cctgtccagc agggataggt agttcaggtt
    15721 catcgcggaa atcagcgacc agtgcatgtc gccgtccgtg actggccaga gcggctgcgt
    15781 cgggcgtgtg acattatgaa atgaggccac cgccgggttt ttgttgacgg ccacgcagat
    15841 atcgccgata tgcagccgag cagggatcat acggttggta cagattaatg acgtggtgat
    15901 cacatcatca ttaaaccgtg agctgtccgg aatgctgccg tccgcatgga caaaggcgat
    15961 actatgttca agcccgcggt gaaacaggga tgttttagtc cggtgatgcc agtagacgac
    16021 ttcccgctgg cggctgtact ccatctggtg ctggaaactt tcaaattcgg gccagacacg
    16081 cgcctggctg ccagggttgg attcagtacc tgaaactttg actttgctgg tgactgtgct
    16141 tatctgaaaa atgtcataag cgtcgggatg ccggtggctg gcgcgcagcg ggtattccgc
    16201 tatccgccca tcaggcctga cgggttccgc ataatgcgta aacaggttaa tggctggcgt
    16261 acagtgcaga cgcagtgagt cccggcgtag cttgatgtcg gcaggtaacg ggcggttaaa
    16321 gtgcaggcgc agcgtaaacg cgctggccgg gaaatcgtcc ggtagtggcg caacatcgcg
    16381 cagatggaaa aaaaagaagg cttcgggata gcagaaatac tcctggagta cccgatagcc
    16441 gctgtggaca tttttcggcc acggcagcag tgcgtcctgg ttttcaaatc cggcggcctc
    16501 cagccacaga tccggcagcg gcaggcggta ctctccggca atcagttccg catccattag
    16561 gcattcgcaa aaccacagat aaagttggtg tcgggtataa tcgtcttcat tacccagcca
    16621 gaacgttatt ttgttcaggt caagcatggc gggggagaca ttgttatctg cggtaaaact
    16681 gatatctatg atgccttctt tcaggctgct ggcgttgtgg gtgtcgagaa tgcgcagtgg
    16741 ttgcagccag atatcgcgcg ccagcgtgaa atgacaggct acggaagaaa cgattttatc
    16801 atgattgtca gacagcacct tctgagcagc cagggcggac ttttctgagc ggcttctgat
    16861 cagttcatta cggcagactt gaacagggac ttttaactga tcggttttgg gttcataggc
    16921 aataaccgtc atggccggaa ctgggcgtaa atagtttgcc cacagcatac ggattagccc
    16981 atgggtgaat tcggggaatt cgtcctcaag tttctgacgc aggctaccgg acataaaggc
    17041 aaaggcttcc atcagacgct ccacgtccgg atcaccctct ttctcggcca gaaagtgggc
    17101 cagatgtggt ttttcctgcg ccagcagttt gccaagctgg cgaagataat ccagctcttc
    17161 gcggtagtaa cgttcttcaa aagccacaga gatcactcca cacaatagcg ttgatgattg
    17221 tccagcaaaa tgtcaaattc aaacactccc ctggtttcgc tgacatcgac cgtagccaca
    17281 atatgaaaac gtagttccac aggggcgtat tcatccggtg ccgcagccgt aacaacaaca
    17341 tcggtaatac gaggttcgta atgtaaaatg cactggctaa tggacttacg gatttctcgt
    17401 ctgaaatcgc tggaggccag cgattcatca tttaaatcag taatccccag ctctggtgag
    17461 ccataacaac tgccggagcg ggtattcagg acctgtcgca ggttacgtct gatggaacgt
    17521 atcagtgctt cttttgtatt ctggtatgaa ggcgggttgg ccgcctcacg aatacgttca
    17581 aaaagactgc tggttggccc tgaatcccgt ctttgcatct gattattcct tatccagtcg
    17641 tccgaccaga ctcaggtcga aactggcacc catgtattta aaatgcggtc tgacagaaat
    17701 cgcgacctgg taccagcctg gctcaccatc cacatcaagt acctcgatct tcgcctgacg
    17761 taatggtcgg tggctgcgga cttcggcagg cggattctcc tggtcggcca catactggcg
    17821 aatccaggtg ttcagctcac gctccaggtc actgcgttct ttccatgagc caagctgttc
    17881 gcgctgaatt accttgatgt aatgcgctaa cctactgatt acaaataagt atggcagctg
    17941 agtgccgagt ttatagttgg tctccgcagc cttgccttcg ggggttttgg ggaatttttt
    18001 gggtttctgc accgagttgg ccgagaaaaa gcacgcatta tcagatcctt tacgcatggt
    18061 cagtgtgata aagccttctt ccgccagctc atactctctc cggtcagtga tcaacacttc
    18121 agtcggaatt tttgcctgca tttgccccat ggattcatac agatgcaccg ggaggtcttt
    18181 gactgcgccg ccgctggaag ggccaatgat attgggacac cagcgatatt tagcaaaact
    18241 ttcagccagg ttcgcagcta gtaaaaacga ggtgttcccc cacaaataag actcgtgact
    18301 ctggctgacg tcttcatggt aacggaaggt cctggctggg ttctcatctg gcgagtaagg
    18361 gtgacgcagc aggaaccggg gggcagtcag tcccaggtag cgtgaatctt cagattcacg
    18421 caaggcacgc cagcgggaat aggccgggcc ttcaaagatt gctcctggtt ctttgatccc
    18481 tggtaactct gtccatgagt ccagaccgaa aaattcggat gatactgatg agaggaacgg
    18541 gctgtgcgcc atcgcactga cctggctgat atatttcagc aacttcatat ccggcgtggt
    18601 gttcttaaag gcaaagttgc cgattaccgc cgccactggc tcaccgccaa attgtccgta
    18661 accgcttgaa tatacatgct tgtaatacgc agactgcgtg atttcaggtg caaattcgaa
    18721 atcatccagc agttcttcct gagtgacatt caggaaatgg accttgatgt tttcccgtgt
    18781 atccgcgcgt tcaaccagca gcttcagcga gcgcagcagg gattccagtt cctgaaactc
    18841 cggggcatga ataatggcat ccatctgcct gctggttcgg gcatcaatat cagcaatcat
    18901 ggcgtcaacg gccaggatat tgataggttc agcgctactg tcgccctgta acatggcggc
    18961 aataaaggcg ctcacgccct ggcgggtgat atcgtagctt tctgatttag gctggacgcg
    19021 ggtttgcgcc atgatttcat caagcagaga tgggggcgcg gcggtttgcg acgcgctgac
    19081 gggagcaata tcttccttta ctgacatact gaaaatcctt ctgtgaaaat tatttctgag
    19141 cggcgatacc cagttcttgg atcagctgct cgcggctgtc ttcgttttcc agtagcgcct
    19201 gaagttgtgc gcggaaggcc ggaatattgc ctagtggaga cttaagtgcg accagagctt
    19261 cacgcaattc gagcagacgt tttatttctg gtacctggct ggcaatatgg tccggagaaa
    19321 ggtcatccat agatttgact ttcaggttga cgtacatttg ctcattggga gcgtcactga
    19381 gttcagccgg aacaacaaac tcccgttcaa tgtcagcttc tgaaatgact gcgttgaggt
    19441 tatagcggtt gatagcaatg gcagttcgct cgtccaacgg cgtgttatct ggtttccctt
    19501 tcagattgcc ggtgatcaat aaattcagcg gcaattcgat atcagcagcc accccatcaa
    19561 cttttggtgt ataccgaacg ctgattcttt ctttcggcgc gacactgccg ccattgttgt
    19621 tcattttgct cataaaaatg ttcccgttca ggccatgagc acagcatttt cgtactgcat
    19681 aaaaataccg aagataccgt ttcagtggca gttagataat gtaaccttcc tt
//
LOCUS       DAYPHN010000086        19610 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000086 DAYPHN010000000
VERSION     DAYPHN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.086"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(149..895)
                     /gene="cutC"
                     /locus_tag="ACG0BW_002878"
     CDS             complement(149..895)
                     /gene="cutC"
                     /locus_tag="ACG0BW_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFK7259814.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(909..1475)
                     /gene="yecM"
                     /locus_tag="ACG0BW_002879"
     CDS             complement(909..1475)
                     /gene="yecM"
                     /locus_tag="ACG0BW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFK7259815.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1691..3424
                     /gene="argS"
                     /locus_tag="ACG0BW_002880"
     CDS             1691..3424
                     /gene="argS"
                     /locus_tag="ACG0BW_002880"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFK7259816.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3477..3869)
                     /gene="flhe"
                     /locus_tag="ACG0BW_002881"
     CDS             complement(3477..3869)
                     /gene="flhe"
                     /locus_tag="ACG0BW_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFK7259817.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIPAIEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3869..5947)
                     /gene="flhA"
                     /locus_tag="ACG0BW_002882"
     CDS             complement(3869..5947)
                     /gene="flhA"
                     /locus_tag="ACG0BW_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFK7259818.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGVLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5940..7088)
                     /gene="flhB"
                     /locus_tag="ACG0BW_002883"
     CDS             complement(5940..7088)
                     /gene="flhB"
                     /locus_tag="ACG0BW_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFK7259819.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7290..7934)
                     /gene="cheZ"
                     /locus_tag="ACG0BW_002884"
     CDS             complement(7290..7934)
                     /gene="cheZ"
                     /locus_tag="ACG0BW_002884"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFK7259820.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7945..8334)
                     /gene="cheY"
                     /locus_tag="ACG0BW_002885"
     CDS             complement(7945..8334)
                     /gene="cheY"
                     /locus_tag="ACG0BW_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFK7259821.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8349..9398)
                     /gene="cheB"
                     /locus_tag="ACG0BW_002886"
     CDS             complement(8349..9398)
                     /gene="cheB"
                     /locus_tag="ACG0BW_002886"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFK7259822.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9401..10261)
                     /gene="cheR"
                     /locus_tag="ACG0BW_002887"
     CDS             complement(9401..10261)
                     /gene="cheR"
                     /locus_tag="ACG0BW_002887"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFK7259823.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHQGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10280..11881)
                     /gene="tap"
                     /locus_tag="ACG0BW_002888"
     CDS             complement(10280..11881)
                     /gene="tap"
                     /locus_tag="ACG0BW_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFK7259824.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFN
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYHISALVFISMIIVAALYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11927..13588)
                     /gene="tar"
                     /locus_tag="ACG0BW_002889"
     CDS             complement(11927..13588)
                     /gene="tar"
                     /locus_tag="ACG0BW_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFK7259825.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIASGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(13733..14236)
                     /gene="cheW"
                     /locus_tag="ACG0BW_002890"
     CDS             complement(13733..14236)
                     /gene="cheW"
                     /locus_tag="ACG0BW_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFK7259826.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNFGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14257..16221)
                     /gene="cheA"
                     /locus_tag="ACG0BW_002891"
     CDS             complement(14257..16221)
                     /gene="cheA"
                     /locus_tag="ACG0BW_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFK7259827.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRNQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILTGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQDTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16226..17152)
                     /gene="motB"
                     /locus_tag="ACG0BW_002892"
     CDS             complement(16226..17152)
                     /gene="motB"
                     /locus_tag="ACG0BW_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFK7259828.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17149..18036)
                     /gene="motA"
                     /locus_tag="ACG0BW_002893"
     CDS             complement(17149..18036)
                     /gene="motA"
                     /locus_tag="ACG0BW_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFK7259829.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMHCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18163..18741)
                     /gene="flhC"
                     /locus_tag="ACG0BW_002894"
     CDS             complement(18163..18741)
                     /gene="flhC"
                     /locus_tag="ACG0BW_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFK7259830.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18744..19094)
                     /gene="flhD"
                     /locus_tag="ACG0BW_002895"
     CDS             complement(18744..19094)
                     /gene="flhD"
                     /locus_tag="ACG0BW_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFK7259831.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4654 a         5464 c         4806 g         4686 t
ORIGIN      
        1 aacgctatgc gcactgtcac gcgctcaact gatcaccgaa atcgttcgtt ttttatactt
       61 ctcggggata aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag caagcatcat
      121 attgggcgac atgatgcaac ggtaaaaatc atttggcctg atggcgttca atgattcctt
      181 tcatttcagc aaccgccgcc ccgtcgacga cataacgcga atactcgtcc gcgtgtgcat
      241 ctgatgacat ggacaatcct tgattacgat aacgcattgg tgaggcttgc cacgctcccg
      301 cggagctatg gacttccagc actccggcat cgaggaagtg gtgcaggttt tctgcacgga
      361 ctcctgctcc ggccataatg attggagcat caccatgggc aataagttcc ataatttttg
      421 ataaaccttg cagtgcgtct gatttttgcc ctgatgtcag tacccgagca acgcccaatt
      481 ccgcaagatt attgagtgta tttaaagggt tagcgcacat atcgaaggcg cgatgaaaag
      541 tcactgccag cggaccggca gcagccatta ttttttccat tcgtggcata tcgacattcc
      601 cgtcaacatc gagaacgccc gtcaccagtc caggaaaacc taattcgcgg actgtgcgca
      661 catcctcaag aatggcggca aattcaccgt cgctgtagca aaaatcacca ccgcgtgggc
      721 ggataatcgg atgcacaggg atcgtcaccc gctggcgcac ggatttcagt acccccaacg
      781 acggcgttaa gcccccctct tttggggctg cgcataattc aactctgtct gcgccgtttt
      841 gctgcgccgt tagcgcgcat tccatgctgt aacagcaaat ttccagtaat gccatttttg
      901 ctccttaatt acgccgactg ctcgctggca acgatctctt caatggacca cggatgaaat
      961 ttaatggtcg ttttgccatc ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca
     1021 ccttttggcg aactggtttt cactgagatc cccggcaggc tgagtccttc atcagaaagc
     1081 aatgccagcg cacgggcatt cagcgtttct ggatcgcccg gcagaacgat ttcaatatgt
     1141 tcccaacctt cgtgcgggta acgtttttcc ccgggccacg gtagctccac aatggaaaac
     1201 tgccagtgcg caacctgtac cggttcatgc aatttaaaca gacaaatcgg cctgccattg
     1261 atcatatttt ctgacaaaag ctcgccgcac tgttcaaacc cgcgacgcca gcgttcagcg
     1321 gtgacgtttt gatggcaacg caaggaaatg tgatcggcag tcagcggagt gatatccaac
     1381 cccagacggc gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata
     1441 tcctgcagct cgtcgataga ttgccagttc gccataatca ctcttcgtct ttcagtaaaa
     1501 gcgttaattt accctgttgc cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg
     1561 caatagtgaa aacggcaata cgccacgcgc acgttgctga cgaaaacagc catttgcagt
     1621 atactcccgc cctaatttct ttaactggtg cgggcaattt ttgctcgctt catcaatgta
     1681 aggtattccg gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc
     1741 ggcaggcgcg cctgcggatt gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg
     1801 cgactatcag gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca
     1861 attagcagaa caggtgctga ctcatctgga tcttaacggt atcgccagca aagttgagat
     1921 cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg atcatgttca
     1981 gcaggcgctg gcgtccgatc gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt
     2041 tgactactct gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat
     2101 tattggtgac gcagcagttc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa
     2161 ccacgtcggc gactggggta ctcagttcgg tatgctgatt gcgtggttgg aaaagcagca
     2221 gcaagaaaac gccggtgaaa tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa
     2281 aaagcattac gacgaagatg aagagtttgc cgagcgtgca cgtaactacg tagtcaaact
     2341 gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac
     2401 gcagaaccag atcacctacg atcgtctcaa cgtgacgttg acccgcgatg acgtcatggg
     2461 tgaaagtctc tacaacccaa tgctgccggg tatcgtggcg gacctgaaag caaaaggtct
     2521 ggcagtagaa agtgaaggcg cgaccgtcgt attccttgat gagttcaaaa acaaggaagg
     2581 cgaaccgatg ggcgtgatca ttcagaagaa agatggtggc tatctctaca ccaccactga
     2641 tatcgcctgt gcgaaatatc gttatgaaac actgcatgcc gatcgcgtgc tgtattacat
     2701 cgactcccgt cagcatcaac acctgatgca ggcgtgggct atcgtccgta aagcaggcta
     2761 tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg gcaaagacgg
     2821 caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc tggatgaagc
     2881 cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag ccgacgagct
     2941 ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa
     3001 ccgcaccacg gactacatct tcgactggga caacatgctg gcgtttgagg gtaataccgc
     3061 gccatacatg cagtatgctt acacgcgtgt attgtccgtg ttccgtaaag cagaaattga
     3121 cgaagagcaa ctggctgcag ctccggttat catccgtgaa gatcgtgaag cgcaactggc
     3181 agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag gcacgccgca
     3241 tgtgatgtgt gcttacctgt acgatctggc tggtctgttc tctggcttct acgagcactg
     3301 cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac tggcacaact
     3361 gacggcgaag acgctgaaac tgggtctgga tacgctgggt attgagacag tagagcgtat
     3421 gtaatcgcgt tatacggcaa aagccgatgt catggcagac atcggctttt ttatcattaa
     3481 cgataattca caatcacttc attacgttgt actttcagcg gcggaattaa ccgcccaccg
     3541 ccaggcactt cccagataaa tcgtaacggt tctatcgccg gtataccgga aaaggccacg
     3601 gtggttccgc tctgcccatc taattcgaca caacgagatt gcgaacacaa gcgaacccgc
     3661 aatcctgcag gtgtcggacc gataagctga taacgccacg ccaccagcgt catcaatcct
     3721 gcagcaggtt gtcgagtaga aagaggcgct gacgacatcg actcaccacg gtgattcagc
     3781 gtaacgccca cactactcgc ctgccacatc ccctccccgg cggcttgcac cagcagcgga
     3841 aacaataata ttgctaataa ggttctcatc atttaccgcc aattgtcgcc gtcatgcgga
     3901 tatgtcggtt atcagatagt tccagattcg acagcaccac taactgcggc aagctgcggc
     3961 gcaggaagcg agacaataat ggtcgcagcg cgtggttcac caataaaact ggcggcgcac
     4021 ccaacatctc ctgacgggat agagcttcct gagtttgcgc cagtaaacga tccgccagcc
     4081 ctggctccag tccgcccccg ccctgcaacg cctgtagcaa caaacgttcc agcggtgtat
     4141 cgaggccaat aacatgaact tcatctttgc caggaaacca ctgctgggta atcgcccgtc
     4201 ccaacgccac gcgcacgacg gcggttaatt catgtggatc gctttggatg ggcgcatgtt
     4261 ccgccagcgt ttcgagaata gtgcgcatat cgcgaatcgg cactttttca tcgagcagat
     4321 tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca
     4381 gctttggcat ctcctgggcg acgcgatcca gcaactgttg cgcctcctga cgaccaaaca
     4441 gctctgcggc atgctggcta atgaggtggt taagatgcgt tgccaccacc gtgctggcct
     4501 caaccacggt gtacccctga atctgcgcct gttctttaag cgcactttca atccagatag
     4561 cattcaggcc aaatgccgga tcgacggtcg cctcaccagg taacgtcccg gcagcggtac
     4621 cagggttaat cgccaaccaa cgccccggat aagcatcacc actgcctatc tccacgcctt
     4681 tcatcaaaat gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg tgcaccactg
     4741 gcggcagaac ccccatctcc tgggcaaatt tcttgcgaat actgcgtata cggcccagca
     4801 actcaccatc ctgctggaaa tcgaccatcg ggatcaggcg ataacccact tccattccca
     4861 gagaatcttc cagttgtaca tcgttccacg tcgcttcgac aacggcatta ttctctgcca
     4921 tttttaccgg ttttggttcg gcaggcgctt tttgttcgcg tccgcggatc caccaggcca
     4981 gcccaagcaa tccggcagtg aacagcaaaa ataccaggtt cggcattcca ggcaccaggc
     5041 cgagtaaacc gagcacggcg gcgcttaaca acataacgct cgggttactg aaaagctgat
     5101 tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg
     5161 cggtagaaat caccaacgcc ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg
     5221 tataactttc cgccgcgtgc cccatgctca tgccatgttg cagcacgccg accagcaacc
     5281 caccgacaac gtttatgacc atgatgagga tcccggcgat ggcatcgccg cgaacaaact
     5341 tacttgcccc gtccattgaa ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt
     5401 ttttcgcttc atcttcacca atcaatccgg cgttaaggtc ggcgtcaatc gccatctgct
     5461 tacccggcat accatcgaga acaaagcgcg cccccacttc tgcgatacgc ccggcacctt
     5521 tggtaatgac cataaagttg atgatcacga gaatgacgaa caccacgata ccgatagcga
     5581 aattgccacc aacgaggaag tgaccgaacg cttcgaccac cttcccagcc gccgccgcgc
     5641 cggtatgccc ttccatcaaa atgatgcggg ttgaagctac gttaagtgcc agacgcaaca
     5701 acgtggtaaa caacagaatc gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca
     5761 tcgccaccag caacaccatg atcgacaagg cgatattaaa ggtaaacagc aggtcgagaa
     5821 tgaatgcagg caatggcagc accatcatcg acaaaatcag caagatcaaa atcggtccgg
     5881 caaggatctg ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc gcggccagat
     5941 tactcatggg tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta
     6001 ggttgtacag ggcgctgtcc accagccaga cgccagcgtt tcagttgcca gacccaggcc
     6061 agcacttccg ccactgcggc gtacagttga cccgggattt gttgaccgat ctccgcatgt
     6121 cgatacagcg ctcgcgccag cggcggcgct tcaagcgtag ggacgttatt ttcagcgcca
     6181 atttcacgaa tgcgcagcgc gaccagccct gcacctttag cgaccacttt cggtgcgctc
     6241 attttgtttt cgtcatactg caacgctacc gaatagtggg tcgggttatt gacaatgaca
     6301 tccgctttcg gcacatcggc catcatccga cgccgtgcag ccgctcgctg catctgacgg
     6361 atccgccctt taacatgggg gtcgccttcg ctttgtttga actcatcacg aatatcctgc
     6421 cgcgacattc gcagcttttt caggtggctg aagatttgga aaaagacgtc aaatcccacc
     6481 atcggaataa caccaagtac caccagcagc gcgcacagcc ctaccagatc catcgcatta
     6541 cccatggcgg taataggaga ctcggccatt aagcgcatca tctgcggcca gtgatgccag
     6601 agaaaaaatc ccgtcacgct gccaaccagg atggttttca aaattgcttt aagcaactcc
     6661 gcgccagtct gtgccgagaa catccgttta atccccggta gcgggttgag tttggaaaac
     6721 ttcggctgca aggatttgcc gctaaatacc agccctccca gcatgaccgg agaaatgagc
     6781 gccaccagca ccacgccgct aatcagcggc agcagcgcca gcatggcttc tctgatcagc
     6841 agaataattt gcccgaggat cagattcggg tcattgatga tgctgtgatc aaaatgcaat
     6901 cccgcggaga gcatgcccga caatcgacgg gccagcgaca caccgccaaa ccagataaca
     6961 cacacgccca ctaacaaaat cagcagtgac gttaattcac gtgaacgcgg gatttgcccc
     7021 tcttcccgcg ctttttctag tcggtgaggt gtgggggctt ctgttttgtc gtcgctctcg
     7081 tcagacacgt cgccaatcct ggaaagagta ttaagcgtaa atgatgccag agcgcaaagc
     7141 gttcaatggt ttgagtaagg ggcaaaacag gcgggattta gggcttttgc tgccacacat
     7201 caagcatagt atgcgtttgt cggatgcggc gtcatcgcgt tatccgaccg cctgatatga
     7261 cgtagtcacg ccacatcagg caatacaaat caaaatccaa gactatccaa caaatcgtcc
     7321 acctgatcct gactggctac cacaccggct ttgctggtat cgacctgagg tccattaagc
     7381 aaactctggt tttcacgttt tggacgcgac tcctgttccg ggatgttttc caacagcacc
     7441 atcaacaact ggcgttcgat ctcctgaatg acatccatca tccgcttaat gacctgtccg
     7501 gtgagatcct gaaaatcctg cgccatcatg atttccagca gttgcgcgtt agtaaagctg
     7561 gtatgcgcgg ggacatctgc cagaaattgc cgcgtatctg ttaccagttc tcgggcgtcg
     7621 gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt gggttaacgc ttttgctgat
     7681 ttctccattt gatcctgatg cggttgtgac gcctcaacac tgttcagcgc ccgctccgca
     7741 gcctgggcgg tcatctgcac aacatagtac aaacgatcgc gcgcatcggg gatggcttcc
     7801 gccgcttcgg caatggcctg atccagcccc agctcccgca aactgtcgcg cagcatacgc
     7861 gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc aggtttgatt
     7921 gatggttgca tcatagtcgc atcctcacat gcccagtttc tcaaagattt tgttgagttt
     7981 ttcctccagc gtcgcggcgg taaatggctt caccacatag ccactggccc ccgcttgcgc
     8041 cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc attaacactg gcaatgctga
     8101 catcgcgcca tccgcacgaa ttgttttcag caattccagg ccatccatgt tgggcatgtt
     8161 ccagtcggag ataacaaatc cataaccgcc tgcctgcaac ttattgagag cgtcgacacc
     8221 atcttccgct tcctcaacat tattgaatcc tagctctttc agcaggttac gcactatgcg
     8281 tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt tctttatccg ccatttcaca
     8341 ctcctgattt aaatacgtat cgcctgtccg gcactaattt ttgccaacat ttgctggctt
     8401 acctggctaa gatcgaccac ttcgcagaca ccacccatat tgatggcctc gcgcggcatg
     8461 ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc ctgacgcatc
     8521 gccaacattc ccgccgcacc gtcgttgccc ataccggtca ggatcacccc aaccgcatta
     8581 cgccccgcct gtttcgcgac agaatggaac aacacatcta ccgaaggccg atgacggtta
     8641 accgccgggc catcgtgaat tttgatttgg taatttgcac cactacgcgc cagctccata
     8701 tgccgatcgc ctggcgcaat ataggcatgc cccggtaaga cacgttctcc gtcttcggct
     8761 tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg ggtgaaaccg
     8821 ggcggcatat gctgggtaat taacagtgcc gggctggaaa gcggcaacgg ttgcagtacg
     8881 tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc
     8941 aacaacggcc ctgccttcag cgttgtcggt gccgacaatg gcttatgtgc tgcaaggctc
     9001 gcctttgctg ctgtacgcac cttttcagca atcatttcgc tatacgccag cattccttcg
     9061 cgaataccca gttgcggttt ggtgacaaaa tctatcgccc ccagctccaa tgcgcgcagc
     9121 gtgacttctg accctttgcc ggtcagggaa gaaaccatca caacgggcat tgggcgcaaa
     9181 cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc
     9241 aatacatcgg gattgaattt cttaatcaaa tcccgcgcaa ccagcggatc aggggcggtc
     9301 gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt
     9361 gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta cttagcgcat
     9421 acaccgtctg accacgcagc gtgaagcggc gctcaaggtg gctaaagttt tcagagtgac
     9481 ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atctcctgct
     9541 gggtagtttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg
     9601 gcacggtgta ctgtttcgcc agtaaattca gcggggcaaa atcaacataa ttcgccagtt
     9661 cctgacgcac gcgtaccagc ccttgatgcg gtcccgtccc tcgcatgaag taccgctgca
     9721 gttgctgcgg cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt
     9781 ccagcacttc ggtgtcaatg tcgctggcaa acactttcca gcgtccgggc gcggtgccca
     9841 atgtgtcagc cagtgtcatc gcaatgctgt acggctcttc gccggtcgaa gccgccgcgc
     9901 tccatacgcg atactcgcca gaacgacgcc gggcgtgatc cgcgagcaga ggaaaatgat
     9961 gcgcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact
    10021 caccgctgtg ctggttagat tccagcaagt tcaagtaatg gccgaaatcc gtcagtccca
    10081 gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg tcggccagga
    10141 cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaatgcgcg tcggaaagcg
    10201 ccaggcgctc ggtcatctgt aacaataaag acgtttgccc acagggcaga gatgaagtca
    10261 tagcgccttc tcaatcactt caggatacca ctggcgcaat ttgtaactgc gccgactcat
    10321 gtcgtgctac ttcatgttct tcaagggtaa ataccgccac acgcgacgaa agatggtcgg
    10381 cctgattcgc cagttgttcc gttgccaccg ccgcttcttc taccagcgag gcgttctgct
    10441 gtgtcacctg atccatctgg ctgacagcct gcgcaacctg ctcaatcccc cgccgttgtt
    10501 cttccgacgc cgaagcaatt tctcccataa tgtcgttcac gcgggttacc gaactgacaa
    10561 tatcagtcat ggtcgcggcg gcgttattca ccagtttcga accctgctgg acacgattga
    10621 ctgactcttc gatcagccct ttgatctctt ttgctgcctg cgcgctacgg ctggcaagat
    10681 tgcgcacttc acctgccact accgcaaaac cacgcccctg ctctccggcg cgagccgctt
    10741 ccactgccgc attcagggcc agaatattgg tctggaaagc aatgccgtcg ataacgctga
    10801 taatgtcgcc tattttttgc gagctggtgg cgatctcctg catggtgtga gtcatggtac
    10861 tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt tccgacgcct
    10921 ggcgcgcgtt atcggcgttt tgccctaccg tggcggttaa ttgctccata ctggcggccg
    10981 tttgtgccag cgatgccgcc tgctgttcgg tacgacttga gagatcatta ttgcctgcga
    11041 caatctccgc gataccaatg tgcatctcct ggcttccctt acgcacatca cttaccgtcc
    11101 cacgcaaagc ctgctgcatg gtcttcagac tggcaaaaat ggcggtgatc tcattgcgac
    11161 catacaccgc aatcggacgc gccagattac ccgcagcaat gctgtcaaaa tggctaccga
    11221 tgatggccag tggttgaaca atcatcttgc gcgtccacca cagtgcactg ctgatgtaga
    11281 gcgctgcaac aataatcatg ctgataaaca ccagcgccga aatgtgatag ttacgctggc
    11341 tttgcgcact ggcagcttcc agcacatggt tgatctccag ctgccaggcc tcaaagttaa
    11401 cgtcaaacgc attctgtgat tcctgcaccg gcgcagtgag gaaatccgaa agttgattac
    11461 tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa cttttctccg
    11521 tctctttttg caacgccctg acgtgctcat tgcccgcagt catcgccata aaactattaa
    11581 acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt ttaatgtcat
    11641 ccgccggata gctaagcgct gtcagagtgc ctgctttgtt cagcgcggta ctggcctgta
    11701 acattactgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc tcgacctgat
    11761 tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg ccaatctgca
    11821 agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata cgattaaaca
    11881 tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa aatgtttccc
    11941 agtttggatc ttgttcagta attcgcagtc gtggctgtgc cggtggttgc tcactggcag
    12001 gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacga aacgcggaaa
    12061 ccgcttgcgt taaacgactg gcctgttctt ccagcgcagc tgcggcggca gctgattcct
    12121 gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc aatgcgactt
    12181 gatcgatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata atgtcagtca
    12241 cgcgagtgac agcattgacg atattgttca ttgtttcccc ggcgctttcg accagcaccg
    12301 aaccggtatc aacgcgcgag acggagtctt caatgagggc tttgatctct tttgccgcct
    12361 gggcgctgcg actggcaaga ttacgcactt cgcccgccac cacggcaaaa ccacggccct
    12421 gttcacccgc acgcgcggct tcaacagcgg cattcaacgc gaggatatta gtctggaagg
    12481 caataccatc gataacgctg ataatgtcgg caattttctt cgaactatcg gcgatctcat
    12541 gcatcgtttt cactacacca tccactactt ttccgccgtg ctgggcagta tcggaggcac
    12601 tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact gtcgcggtga
    12661 gctgctccat gctggcggca gtttcttcca gcgcagatgc ctgctgttca gtacgggagg
    12721 aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca tctgaacctt
    12781 cacgcacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg ctctgcgcca
    12841 ggtcgcccat ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta ccactggcga
    12901 tttcgcgaat gtgagcaata atttttgcca gcggtgtaag caacatacgg cgaatgccgt
    12961 accacgccac cagcagaatc aataccacca ccagcgcgat aaccgccagt tgccactggg
    13021 caaatcggta atcatctgcg ttgtcagtga cgatatcgcg atacagtttt tcactgctga
    13081 gggcgtactg agcaaacgct tcgcccattg cattttgcat tccctgggtt ggctgagcga
    13141 aataagctcc agtattgcca taatcaagat aatcaatcag ttcagttaac gctgtgtgat
    13201 agtttttata tttttcatca atattacgac tggtagcgac catttcaggt aacggtgcca
    13261 tgcttttgaa ttttttataa tgcgtcgcgg cctgggccaa tgttttcctg gcgctatcga
    13321 gcaattcaac tttggcgtta ctttgttgat tggaggaatc catcatcatc cgcaccgctg
    13381 aacgactcag gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc agctcgccct
    13441 gctgttcccg taattgattg gaaaccacaa agctcttctg gctatggtga agggaagaaa
    13501 aaaacagact gccggaaata agctgtaaca gtgcgaatac ccccagcacc attaccaaca
    13561 gcgtgactac gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag ttgatctcgt
    13621 tatcggcaag gaagggggaa actttattgc tgatgccacc cgccgcgaaa ctgaaataaa
    13681 aaacccgatg cgcaggtcat cgggttcatt gcaattgagg aaatcgggag aattacgcca
    13741 cttctgacgc cgcgctatct aacagcgcca tctcttcgct gttcagcagt ttttcgatgt
    13801 tcaccagaat caacatccgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa
    13861 gcgtcacggc aaattccggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg
    13921 aaacgccgtc aaccacgatg ccgaccaccc gctgtccgaa attcaggacg ataactaccg
    13981 tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtcg acaatcggca
    14041 caataacgcc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg
    14101 ttacctgatc gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat
    14161 caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat
    14221 tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg
    14281 ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc
    14341 gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt
    14401 aaccacaacc tggtgttgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc
    14461 actttgtaag atcaccacaa ttccctgggt agcttcggtt ttcgcgcccg cgacgttgaa
    14521 cactttccac agttcgacga tgggcagata ttcaccccgc acttccagca cccgctcgcc
    14581 gcctgccagt ggatgcagat cggcttcacg gggttgcagt gattccataa cagcattcag
    14641 cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt
    14701 tagcggcagt aaaatgcgga tcgtagtgcc agtatcctgc ttcgactgga tttcgacatg
    14761 accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccagagac
    14821 gtcggtgacc tgctctgccg tggagaagcc tggtgcaaat atcagcatcg cgacttcgtc
    14881 gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc
    14941 acggtttagc cctgcaccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc
    15001 ggcagaaaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag
    15061 ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc
    15121 tatcaggctc ttgtcgagtt cagtagaact gcccaccagc gtcagttcta cctgcttgcc
    15181 gagttttccc gccagatcac gcaccagccg gggataacgg ctaaagacat attccatcgg
    15241 catcatgcga atcgacatca ctgattcctg caaatcacga gcgttacgtt gtaactgccc
    15301 catgctggta atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag
    15361 catggactgg gtgatgacca gctcgccgac gaggttaatt aattgatcaa ccttttctac
    15421 cgctacacgg atgctggtgg attcactgct gcgcgtcgtt ttttcccgct ccacgcgacc
    15481 ggttggcgct tgttcagctg ccagtttaag cactggtggg ggggatattt tgggcgagac
    15541 ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat
    15601 gtcatcttcg gcgatgtcgc ccgttaatat tgccgagaga gaatccgccc ctttcaccac
    15661 gtcggttaac gttgtcagat gccccagctc ttcttccagc aggtcgactt ccccggcctt
    15721 caggcgcgaa aggataattc gtcgcggcga ctgattgcgg ctctgctcat cttgcggttc
    15781 acttttggca accacactta atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa
    15841 tgccagttga cgcaaggcct ggcagatata atcgaagctg gcggcatccg gctcttgcga
    15901 ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat
    15961 aatgtcggtg ttgagttgca tctcacctcg tctggcttcg tcgagcaggt tttccatcag
    16021 atgcgtggtt tcctgcaaaa cgctgaagcc aaaggttcct gcccctcctt tgatcgagtg
    16081 ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag
    16141 cagatgctgc tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa
    16201 atcgcttata tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac
    16261 tgacctgtgg tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg
    16321 cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac
    16381 ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacga
    16441 cacgtaacac tttgccgcta tccaacccgc cgaccatcag ttcgcggcgg gatgcattgg
    16501 cccgatcggc agaaagctcc cagttgctgt aacctttctc accgctggcg tagggaaaat
    16561 catcggtatg acctgaaagg ctaatacggt tgggaatacc gttcagtaca ggcgcgatgg
    16621 cgcgcagaat gtcgcgcata tagggttcca catcggcact gccggttctg aacatcgggc
    16681 gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat
    16741 gtggacgtaa cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga tcgccacgca
    16801 atttccgcaa tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc ggctgcttat
    16861 tcacttcccc ctggctttgg gtgtaatcat cacccccacc gggaattggg ctttcactat
    16921 tagaaatgcg atcgccgccc gtaaccgcag tcgccagtgg agtccggaag tactccgcaa
    16981 tctgaatcag ctcttttggg ctggagatgg agattagcca catcaccaga aaaaaggcca
    17041 tcatcgcggt cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc
    17101 ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc
    17161 ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa
    17221 cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc
    17281 gtaaccgttc agattagata gcagtgtgac tttgacgcag tgcatcattt tgctggtttc
    17341 ggcgcttttc tgacgtaaaa cactcgctaa tggggaaata aatccgtaag ccaataaaat
    17401 gccgaggaaa gtccccacca tcgcatgtgc gataagcgcc cccagctcgg cggcaggacg
    17461 atcggctgaa cctaacgcgt gaacgacccc catcacagcc gcgacaatac caaacgccgg
    17521 aagtgagtcc ccgaccagcg ccagactgtt cgccgggact tctgcttcgc tttcgtgcgt
    17581 ctcaatctct tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat
    17641 cagacgcaga taatcgacga taaaatcaag catgacgcta tccgcaagga tgcgtgggta
    17701 gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc
    17761 catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca tatacattgc
    17821 tttggtgtat ttggagcgac gaaacagcaa cggcagcgcc ttcagcgtgc ctttaatcgc
    17881 tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa tcaccagttc
    17941 agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt
    18001 accgagaaca accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc
    18061 atgacaggat gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt
    18121 acgtcgttac cgctgctgga atgttgcgcc acaccgtata agttaaacag cctgtactct
    18181 ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt
    18241 tactgcccgg gatggcggtt gacataagct gcaggcaaag ctgccaacag gctgatgagc
    18301 gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc
    18361 aacaaaccgc accaatgtcc aggcacgggt taatgccagc agcggtcctt cttctgcttg
    18421 cgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa
    18481 accggttttc agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc
    18541 ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg
    18601 cagttcttta taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat
    18661 ctgcaaacga gcgcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg
    18721 aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga
    18781 ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg
    18841 tcaacgcggg aatcttgcgt caactgagta atcgtctggt ggctgtcaaa acggaagtga
    18901 caaaccagct gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaat
    18961 gttgtcgcca tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca
    19021 atcagacgct gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac
    19081 tcggaggtat gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg
    19141 caccccgtga tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga
    19201 taagctgcaa taagcagagc cacctttttg gtttaataag tccttacaaa tagaaggggg
    19261 gtctttacac ttatctaaga tttttcctaa atcgacgcaa ctgtactcgt cactacacgc
    19321 acatacaacg gaggggggct gcgattttca ataatgtgtg atgcagatca cacaaaacac
    19381 tcaattactt aacataaatg tataagtcat acttttgttt tgggtgtatt tcaatctgtt
    19441 aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag gaataatcga aatgggatgt
    19501 tgcgcacagt taaaataact caccgtaaat aatcatctgc tataaataat cacttttatg
    19561 caataccaga taagctattt ttaaacagac acttaccgca caacaaacta
//
LOCUS       DAYPHN010000087        19402 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000087 DAYPHN010000000
VERSION     DAYPHN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.087"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..244
                     /locus_tag="ACG0BW_002896"
     CDS             <1..244
                     /locus_tag="ACG0BW_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259832.1"
                     /translation="EKYCKKYNLNGSLAYKITAIKFLPDGMKSYGLDIPLWMVVRDVK
                     GWDDMEITIFISDKNSEVLCCSNIWYPVYRPHQADL"
     gene            complement(350..4252)
                     /gene="hrpA"
                     /locus_tag="ACG0BW_002897"
     CDS             complement(350..4252)
                     /gene="hrpA"
                     /locus_tag="ACG0BW_002897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFK7259833.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRQRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILDAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4453..5058
                     /gene="azoR"
                     /locus_tag="ACG0BW_002898"
     CDS             4453..5058
                     /gene="azoR"
                     /locus_tag="ACG0BW_002898"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFK7259834.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADKITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(5112..6428)
                     /locus_tag="ACG0BW_002899"
     CDS             complement(5112..6428)
                     /locus_tag="ACG0BW_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFK7259835.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGFLNQFTAVQDLKSHDI
                     PSQVFGWETAIPFLPWTIFPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMIMLQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVPDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSRIMLKEEHKAMLKLWANR"
     gene            complement(6418..8175)
                     /locus_tag="ACG0BW_002900"
     CDS             complement(6418..8175)
                     /locus_tag="ACG0BW_002900"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFK7259836.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNVFGRFIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAISDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRYEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8191..9087)
                     /locus_tag="ACG0BW_002901"
     CDS             complement(8191..9087)
                     /locus_tag="ACG0BW_002901"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFK7259837.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVFVGDPSGCLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     INIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(9087..9692)
                     /locus_tag="ACG0BW_002902"
     CDS             complement(9087..9692)
                     /locus_tag="ACG0BW_002902"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFK7259838.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKLQVSANQITLAALALSLLTGL
                     LLALVPYPGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AIAIYPQWMQWNNFLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(9861..12167)
                     /locus_tag="ACG0BW_002903"
     CDS             complement(9861..12167)
                     /locus_tag="ACG0BW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFK7259839.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNLMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLSYNQAMQLANDSSNDVVTSLASKLAEMKHHGQLLRM
                     TPQESDKIAVYLHQKFENDDDLIRVLFLALPDNLQFNFVKKMEKKFPAYFCCGDMQVI
                     HSDAALQRLLTRFNDPAGWSNLAKNQYLSTAMKQKIWHRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATSLIRSEGWDFLESALVSWDNLPAVVLKELQQNMPR
                     NDIWAKFFLKQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPSAALAAEIDPEWWIMAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELH"
     gene            complement(12231..13091)
                     /gene="pdxI"
                     /locus_tag="ACG0BW_002904"
     CDS             complement(12231..13091)
                     /gene="pdxI"
                     /locus_tag="ACG0BW_002904"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFK7259840.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIVEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(13299..19208)
                     /locus_tag="ACG0BW_002905"
     CDS             complement(13299..19208)
                     /locus_tag="ACG0BW_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFK7259841.1"
                     /translation="MQRKKLLSVCVAMALSGQTWATDTSILDITDDTRKSSKITCPAN
                     IHSISKEQLAKLPSECRDSDDSAVMSWTAAGLVAVVTGAAVMALNDGDHHDSSEPDDG
                     GDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPD
                     DGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDVTPPKEPKIFNNSVTFDEDKGTLQ
                     IRNATFTYSKNSDGSYTLTAEDGRTSIVQGWDVDTDANTVEITGVNNLGGMTWCYGKD
                     GIIYITKTVGATVDDPANSNVFNLSDAVLTDQGGNAALNGATVIEINGSRIALNNDGD
                     ISATGKDSVVVAITGNDITVNNNGHMVVDGGTAGVVNGDRAILNNRGDAVITNGGTGV
                     IVTGDNAVINNMGQSDIDGDNSVSVKVAGNATRIRMEGGINVSGGAHGIDATGDNNEV
                     SNKGNISVVDAHSTGVLLNGDRASFINMGDINVSGGAADDHSNGVQINGDNSTFINVG
                     DLNADDRATGVKINGDASDIALAGAMHVGDFSTGLEVMGKNNDLSLSTNMMDVTGKQS
                     TGVTITGDDNTIDITGDMVVDQNSVGAKIVGDRVSLQQKGDITVNGAGHGVEVSGSKT
                     TINNQGKLTVKDQDSIGIVITGDDAQFTTVGEIDVSLNGTGVAISGDREQVNLSGDIN
                     VIQERDGSGTFQGGTGISIMSNDSSMLLTGNINVTSSMGDQPSTSPQSLTGVTIEGEN
                     NTVDLEGDINITVDSDYLGDENSLYGVVVLGTNNTINLDGGINISGDSGGHTIKGVQV
                     TGNNTVNISGHSVMNTRQILGSFSLISVADGGNVIFDENAITEIQSSVRDSDYLFFDA
                     SLIVATGNQSVIQNNGIINTTDAQELMMANSGAQVVNAGVINIRPDAESHTFFAGMMA
                     RSDDSQAKNASGGTINLTSITRPFSSGGFNERPIKWYHNTGYALLASNYGSVINETGA
                     TINLHGAGTYGVSASKGTATNAGEINVDGFVPIVDENGNIIDETYWQTNSVYLMGGGM
                     LAGSTDTGDGDAKAVNTGIINVKNEGFGMLAMNGGTVVNQGTINLTADEGVTKQQDNQ
                     LFAMGAIQGGLAINDQDGVININTDIGQAFYKDGTGTILNYGKINLFGNPMDESDSHI
                     GVTPDDKDILSELSGSGESISKTTTSDGFIAVNNLANYGDETLNGDVIANGWIFNQPD
                     ASLTINGELSVNQGLENSGHLDVDSINSKTTIYNRETGSITTDLLTLNGAVSFFNEGE
                     FNGSITGDSYQQNVVNTDEMTVTEDGHSLVNGSFLFFNEAGATLTNSGNAVTGGENAI
                     IHVTRTSDSVSQVNRGTITATNGYSAIKTENTGSNSNGKWIWNTETGVINGINPAAPL
                     VDLGRGYNFANAGVINVQGDNAVGISGGTTSYVVQLVNSGIINVGTEQGQLDGTNGEG
                     LIGIKGNGRETTINNTQTGVINVYADNSWAFGGQTKAIINNGEINLLCDTGCDIYAPG
                     TTGAQNDHNGTADITVPEASTTPPQGNVPTPPVDSNAPQLLSNYTIGTNSDGSSGTLS
                     ANNLVIGDNVSVNAGFSAGTADTTVVVNDVFKGENISGVDNIVSSTVVWNAKGSTDAS
                     GNVDVTMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAELNSALKQVSG
                     SQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNNTEYDMLALRK
                     TIDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSFDNGMNWNNAL
                     RYDVHNLDSSRSIAFSNTNKTADTDVKQQYLEFRSEGAKTFEPSEGLKVTPYAGVKLR
                     HTLEGGYQERNAGDFNLNMNSGSETAVDSIVGLKLDYAGKDGWSASATLEGGPNLSYA
                     KSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDAYHWKEDGISD
                     KGVMLNFKKTF"
BASE COUNT         4930 a         5167 c         4316 g         4989 t
ORIGIN      
        1 agaaaaatat tgtaaaaagt ataatcttaa tggctcgctg gcatacaaaa tcactgctat
       61 taaatttctt cctgatggta tgaaatcata cggccttgat attccattat ggatggtagt
      121 cagagatgtt aaaggttggg atgatatgga aattacgatt tttatttctg acaaaaacag
      181 tgaagttctt tgttgcagta atatttggta tccagtatat cgtcctcatc aagcagattt
      241 gtaataagca cgtaaggagt atctcccttg ctatttttca gaattcataa agaaaaaacc
      301 cggcgctaaa tgcttaccgg gttttagatt tatcaggcaa atagcagtgt taaccgctaa
      361 tctgctccat cgcctgcaaa atacgcttat ctgaaatcgg ataaggcgta ccaagttgtt
      421 gagcgaagta actaacgcgc agctcttcta tcatccagcg gatctctttc acgtcttcat
      481 cctcacgacg tgcgggcggc agtttgttga tccattgctg ccacgcctgc tggacatttt
      541 cgactttcag catctgagct cggtcgcgat gtgggtcaac cgccagtttt tccagccgtt
      601 tttcaatcgc ctgcaaataa cgcagcgtgt cgcccaaccg tttgaagccg ttaccagtga
      661 cgaaaccgcg atataccaac ccgcccatct gcgctttaat gtcagaaagc cccagcgcca
      721 tggtcatatc cacccgccct ttcagacgtt tattgatatt gaacaccgca gtaaggattt
      781 gctcgacctg cttcgcaata tccaccaccg tgtcgttcag ttcagcacgg actttttcat
      841 gcagcgcagc aaaaccttct tccgtccaga ccgggccacc attggcgtcg atcagttgat
      901 ccacaccgca ggagatacag tcgtctatca gctccagcac tttgccatac gggttaaagt
      961 acagcccaag tttggctttg tttggcaact tctcatgcaa atacttgatc ggcgatggaa
     1021 tattcagcag cagtagacgg cgaagaccgt tccacattgc ctgtttttgc tccagcgggt
     1081 tatcaaacag tttgatcgcc acactgtcgc gctcatccac caacgctggc cacgccttca
     1141 ctttgtagtt gccacgcttc tgttcgtagc tttccggcag ctgaccaaaa ctccagatat
     1201 gtaagccact ctgctcgata ccgtcatccg ccacggcaga tagcgtttcc tgtactttgc
     1261 ctttcagagc atctttcaga tcttgtaatg agcgcccttc ttttagcttc ttgtttttgt
     1321 catccaccac gcggaatgtg attttcaggt gatcgggcac ctgatcccag tgccagtctt
     1381 cgcggtcaac ggtgacgcca gtcatccgcc gtagctctcg ttcaaggctg tcgagcaacg
     1441 gcaattccag cggtgtgacg cggcctaaaa acgcttcggc atagtttggc gcaggtacaa
     1501 aattacggcg tactggtttc ggcaatgatt taatcagtgc gatgatcagt tcacgacgca
     1561 ggccggggat ctgccactca aacccactct cctcaacctg gttaagtaac ggcagcggga
     1621 tatgcacagt cacaccgtca gcatccgcgc cgggctcaaa ctgatagctc aaacgcagct
     1681 tgagattgcc ctgatgccag aagttcggat aatccagctt gctgatcttt tccgcccctt
     1741 ctttgatcaa catgctcttt tcaaagttga gcaaatcagg cgtttcgcgg ctgacttttt
     1801 tccaccagct gtcgaagtga cgtgctgaga tcacatcgtg gctgatgcgc tggtcgtaaa
     1861 actcaaacag cgtttcgtca tcaaccagaa tatcgcggcg acgtgatttg tgttccagct
     1921 cttccacttc ggcccgcagt ttcaggtttt cacggaagaa tgcgtgacgc gtctgccagt
     1981 caccttccac cagcgcgtgg cgaataaaga gttcacgaca taacgccgga tcgatctggc
     2041 tgtagttgac cttacgcgca gcaacaatcg gcaaaccata aacagtgact ttttccgttg
     2101 ccatcaccgc gccctgcgcc cgttcccagt gcggttcgct gtaggtgcgt ttaatcaaat
     2161 gctgagcaac gggttctacc cattcagggt cgatacgtgc cgcaatgcgc ccccacaggc
     2221 ggctggtttc taccagttcc gccaccatta cccatttcgg cggcttttta aataagccgg
     2281 aaccggggaa aatggagaaa cgcgcgttac gtgcgccggt atattcttgt ttatcggcat
     2341 ctttcatgcc gatatgggaa agtaaacccg tcagcaacgc aatgtgaatt tcacgatact
     2401 ccgccggttc gctgttaacc ggaatgccaa gttctttcac cacctggcgc aactgggtgt
     2461 agatatcctg ccattcgcgc acgcgcagat agttgagata atcggtacga cacaggcgac
     2521 ggaaggcgtt ggaagaaagc gccttttgct gctcgccaag ataattccac agatttacaa
     2581 acgcgaggaa gtcggattct ttgtcgtgga agcggcgatg tttttcgtcc gatgcctgct
     2641 gtttatccat cggacgctcg cgcggatcct gaatggagag cgcggacgtg ataatcatcg
     2701 cctcacgcac gcagccatgt ttttgcgctt ccagcaccat acgcgccaga cgaggatcga
     2761 caggcaattg cgaaagctgg cgaccgagcg gtgtcagttt ataggcgctg gcctgttcat
     2821 ccgtggtgat cgcgcccagc tcttcgagca gacgcacgcc atcctggata ttacgtttat
     2881 ccggtgcttc gacaaatggg aacgcggcga tatcgcccaa ccccagcgcg gtcatctgta
     2941 aaataaccga ggccaggttg gtacgcagaa tctccggatc ggtaaactcc gggcgcgaga
     3001 ggaaatcgtc ttcggaatag agacgaatac agatcccttc ggacacacga ccacagcggc
     3061 ctttacgctg gttggcagac gcctgcgaaa tgggctcaat cggcaaacgc tgcactttgg
     3121 tgcgatagct gtagcggctg atacgcgccg tacctgggtc gataacgtat ttaatccccg
     3181 gaacggtcag cgacgtttcc gcgacgttgg tcgccagcac aatacgccgt ccgctgtgcg
     3241 actggaacac gcggttctgt tcgctgttcg aaagccgcgc ataaagcggc aagatttcgg
     3301 tatggcgtaa gttcagcttg ttcagcgcat cggcggtatc gcgaatttcc cgctcaccac
     3361 tcataaagat cagaatgtcg ccagggcttt cctgactcag ttcgtctacg gcgtcaaaaa
     3421 tcgcctgcaa ctgatcgcgc tcggtgtcat cggcttcttc aacaatcggg cgatagcgca
     3481 cttccaccgg ataggtccga ccggagactt cgataatcgg cgcattatta aagtggcgcg
     3541 aaaagcgttc cgggtcgata gtcgcggaag tgataatgat ttttaggtca ggacgccgcg
     3601 gcagcaactc tttcaaatag ccgagcagaa aatcgatatt caggctacgt tcgtgcgctt
     3661 cgtcaataat gatagtgtcg tactgcatca gcaggcggtc ttgctggatc tccgccagca
     3721 ggataccgtc ggtcatcagc ttgaccatcg tgttatcact tacgtgatcg ctgaaacgca
     3781 ctttgtaacc gatgcaaccg cccggctccg ttttcagctc ttccgcaata cggttcgcca
     3841 ccgttcgtgc cgccaggcga cgcggttggg tatgaccgat cagcccttta atcccgcgcc
     3901 ccagctccat acagattttc ggtagctgag tcgttttacc ggaaccggtt tccccggcga
     3961 cgatcaccac ctggtgatca cgaatcgcgt cgagaatgtc ctgctttttc tgactaaccg
     4021 gcaaattgtc gggataagtg atttccggtc gtgccgcttc acgcagcagg actttccctg
     4081 ccgcctggtc aatctctttc gccatctcct ggaaaatggc ctgttgtgca tcaggatttt
     4141 taaccttctt aacaccgtgc agacggcgag aaaaacgctg tctgtcgcgc agcatcagcg
     4201 aatccagccg ctgctgcaag gccgtaaagg tcaatttttg ttgttctgtc ataacgttag
     4261 tgggcagtag gctgcctgat taattttctt ttaaatgata gatatagagt accacatcgc
     4321 ggctttctat gcgttgttca ataaattcga acataggctt cgatttattg cgctatctct
     4381 gtggcaggat tgtgaataaa gtgtcagcaa gcaacggggc atcgcccatt caaacatcta
     4441 taaggaaaca ccatgagcaa ggtattagtt cttaaatcca gcatcctggc agggtactct
     4501 cagtctaatc agttgtccga ttattttgtt gaacaatggc gcgaaaagca ctccgctgat
     4561 aaaatcaccg ttcgcgacct ggctgcaaat ccgattccgg tgctggatgg cgaactggtt
     4621 ggtgcgctgc gtccgagcga tgcaccgctg actccgcgtc agcaggaagc tctggcactt
     4681 tccgatgagc tgattgccga gctgaaagcc cacgacgtta tcgttattgc ggcaccgatg
     4741 tataacttca acatctcgac tcagttgaaa aactattttg acctggttgc acgtgcaggc
     4801 gttactttcc gctataccga gaacggtccg gaaggtctgg taacgggtaa aaaagccatc
     4861 gttattacca gccgcggtgg gatccacaaa gatggaccaa cggacctggt gacgccgtat
     4921 ctgtccacgt tccttggctt tatcggcatt actgatgtga aatttgtctt cgccgaaggg
     4981 atcgcatacg gtcctgaaat ggcagcgaaa gcgcagtctg acgcgaaagc agctatcgac
     5041 agtattgttg ctgaataacc ggacacgatc ttgatgtgga ctgtagatat tcagtccaca
     5101 tctcaatccg cttacctgtt tgcccataat ttcagcatcg ctttgtgctc ttccttaagc
     5161 ataatccgcg agcgtctggc tcgaatataa ctaatcgctt catcaacggt tttacaatgt
     5221 ccgtaacata acagccatgc cgccactacc agcgcactgc gcgataatcc caacgcgcaa
     5281 tggaccagaa cgctgccttg ctcttcgcgt aatgtttcca gcatcgccac ggcctgtcgg
     5341 agctccccct cttccggaac caccagatcc agcatcggta cacagaaata gagtcgatct
     5401 tttgtcgctc gtccccgagg gaattcaaag gtgacgtccg gaaccgcatt ctgtgccgga
     5461 atatgtcgtg gaaacgcccc taaataaaca ccagcagtaa ttttgctcac cggctccagg
     5521 cgacgacaaa accaacgcat agacagccac atcccgatgc gccagggcaa tgtcagccag
     5581 taaacggcgg gcgggagttt cccctgacta tctttccctg ttgttatcgc gccaagccca
     5641 ccgtacccac ggccaataat gagtagcgat aatactggcc aacataacca gactgaccac
     5701 cataactgaa gcattatcat tagctccatc aacacaatgc acgagcccgc gcctacgaca
     5761 tatggcagtg ctattttgat tcgacgttga tcaggtttct gataattcca acgacgatcg
     5821 acgggcacca tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa
     5881 tgatgctgcc aggtcgtcag agtagaaata gcgatgagta aaaaccatcc gccacagact
     5941 ttacgccacc tctcagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata
     6001 atatgcagcg aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaatagc
     6061 catcccgtca ccccactcac ttcaggacgg ataaaactaa acttcagcgg atagagcaaa
     6121 aaaccgcagc aggccattac cgttgccaga ataagccggt ggacaagtcg gcgctgctca
     6181 aatgtcgagc tacaaacgaa cagcgaaaat ccatataaaa gatccagact ccagtaagga
     6241 aaaatagtcc agggaagaaa agggatcgcc gtttcccaac cgaatacctg actggggata
     6301 tcatggcttt taaggtcctg aaccgcggtg aactgattaa gaaatccata ggtgaagaag
     6361 aaaaacgggg ccagcaacaa taaccagcca gctccttgta gcaatacgtt ccggcgttca
     6421 gttatcacga cgcaccgcca tcgaaaccgt aaaaatgccc cactcatcaa tccgttgtgt
     6481 gcatttatca aatccggcat catgcacgag cgaatccatc tccccttgcg aacgcacgcg
     6541 catcacccac ggtttaccat ctttatgact ggttaacacc ccggcaatca gctccagttg
     6601 tgggtgccac ggctgcccgg tgtagatgag aatgccaccc ggttcgatgg cattggcaag
     6661 acctgcgagg gagtttttta cctgctcatt ttcgggaaaa agctcataca gtccagagac
     6721 aatcgccagc gtaggccgcg gagttaacgc gctgagttcc tccgggttaa acgcatcgcc
     6781 ctgttcataa cgcacccgcc cagacattcc ccgttgggca atcatctctt gcccctgtgc
     6841 aacatttaac tcactgtaat cacgtaacaa aatatcgctt atggcgggct cgtttgccag
     6901 cgcatccagt acatagcgcc catgccctgc ggcaatgtca accacgcgga cggctaaacc
     6961 tttggcgtgg agatcggcaa cggcttgttt aatcagcatt tgtaaatggg ttttgcgctg
     7021 gcgaataccg cgccagccaa cactgttcag gtagattttg tcgataaagc gcccgaatac
     7081 gttactgcct tgcggttgat tttgatagac ataatccagc gaactgcctg aatcaaagcc
     7141 ggtgctcatt ccgagatgca ggcccgcaga gtgtgccccg aacagtttca tcgcctttcg
     7201 cataaagcga tacgccaaat caaccggcga taacggcacg ggtccaccag aaaggagccg
     7261 ccatcgatcc gctgatggtc ctgtgcggtc ttcatgctga taatcaaatt tttgcgactt
     7321 gttagcatat aaacggctga taaagctttg cattttttca aatgcctgcg ccctgttctc
     7381 ttcacccaac gtgtcgtgat aaaagcctgg cagcagatgc agctctttga gagggctacg
     7441 taaacgctga taaaaatcaa tctgcggttg gcgatgcacc acatagtcat cgcctgatat
     7501 cagaagttgc gtggggagcg taatcgccgc cgcatctcta ataatacgtt cagacgtttt
     7561 gtagagatcg agcaagatat tgacggcaat cgcccgtgtg atcagcggat cattattgaa
     7621 actcgccacc cgttgccgat cgtgggtcag ataacgccct ttcacatagg aattaataaa
     7681 aaacagacca cgcagacgat gccataacgc cagtccagga cgtgccagcg gcacatacaa
     7741 tttaacctta aaggccggag atgccagcac cagtccgcga atagcaggcg cataatcatg
     7801 aacccatgtg gcaaccatca ctgcgccgac gctttgcgcg atcacaacca cctcttccag
     7861 tccaacctgg ctgtcgctgg cagcaaaacg gacaaattca tcgacatcct gcactgaacg
     7921 cgcaagagat gggctataac cacgcggccc tgacgttttt ccatgccctc gggcatccca
     7981 ggcataaaaa gcagtatctg gcatcgccag ttcatcaacg agatgttgta gacgcccgga
     8041 atgttcatga ccgcgatgaa ataagactat gacctttttc gccccgggct gtaaagcggg
     8101 ccagtgccga taaaacaacg ctgtattatc actggtggta aaaaaatgtt ccccagggat
     8161 gcgtgaattt tccattttct ctattttcct tcagtaacag cagtagcgaa taaaataaaa
     8221 aaataccgga gcggtgaaaa ttaatgagtc aatcctgtca aggagtccgc catgtccggg
     8281 taatagtttt ccactatctt taactccaac atctcgtttg atggctgaca tcactacatc
     8341 gccgcaaaaa ccactaatac caattaacaa acccgctaat aatgcctgta atgtatttag
     8401 cggtgtcagt aacggtccaa taatcagtga cacaatcata gtagtgatta cgccccccac
     8461 cagaccttcc agcgtttttc ccgggctgac tttggggacc acttttcttc tgccgcagga
     8521 tttcccccat aaatactgtg caatatcgtt tgactcggtt aatgccaaaa gaaataacac
     8581 cagtaacgcc tcgccctgga tatttatggt tggcaacatt aacaaccagg cggcatgact
     8641 caaggcgaaa acggtcatta tccagcccca aaaaatggcg ctcacggtat gcagacaacc
     8701 ggaggggtca cccacaaaaa ctcgccaggt ggctaatatc agaaacccag ccaaaggtat
     8761 aaataacaga aacagctcaa aacaattaaa accgataagc aaatagttaa gaggaatacc
     8821 ccaatataac caacgcggaa aatgtacaga tataagcgta caatattctt tcaatgccag
     8881 aaaacttatc agcgcaaaaa acgtcaacgt catccagcgt ggacctgaaa tcaccagtga
     8941 aaaacaaatg acgatgcccc accaggtacg aatacgtaat gtaacctccc ccccttttct
     9001 ctccggtaac cgccatacca gaaaacggtt tatcagcgtt gctaaaatta acaacgcgaa
     9061 aagtgttgcc agagattttt ccagcattat ctttcagcac tcataaaaag cacactccga
     9121 caacgattaa tcgcagtcca gagaagcaga attgaagcaa tgctccataa aaaattattc
     9181 cattgcatcc attgtggata aatggcaatg gccagccccc acagaccaaa tattaacgcg
     9241 cgatcgcttt tgccaaatgg accgacataa ctacgaatac cgttaatcgt ctgcgcgagt
     9301 aaaccgcaaa actcggtcaa tatggtgcaa aataacatga gtattacgag tgatgcgtta
     9361 ctttccggta aaaataaaaa cggtaaataa agcgcaatat cggaaataac atcgcctgtt
     9421 tcattcaaaa tcgccccaag acgtgtttgc tgattgcact cacgcgccat catgccatcc
     9481 agcgcattga gtgccatacg gacaaaaagc acgatgggca atagcagaaa catgccagga
     9541 tatggcacca acgccaacaa caaaccggtg agaagagaaa gtgccaaagc ggcaagtgtg
     9601 atctgatttg cagacacttg cagcttatga agccaaaaca tcgtcggcct taacagcgac
     9661 tgaaagagcg gttttatctg gtagagcgtc attatcaatc cttgataata cggtttgcgc
     9721 tatggtgcat caattgccct ggcagagata tcgcaacacg ccgcttcatt aaaaaatcag
     9781 aatatatcaa ggtactgggg ctcactgtca ggaatgatta tccccaatta atcaaatagt
     9841 taaaaatata acgctgttat tcaatgcaac tcatcaagtt ttctcatcgc ttgttcccgt
     9901 attgcgcgcg tcttaccgtt aagcgccagc tgccgaacta aatccagttc agggttaaca
     9961 agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc aactggaaaa
    10021 cggggattat tcatcgccat aatccaccac tcgggatcaa tttctgctgc cagtgccgca
    10081 gacggtgtat ggcgattgtt aagtgcgccg aacttgaaaa atgtaagatt gcttttcgcc
    10141 agcgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
    10201 tcggggtcta gtgcataata aacacgtaac gcttcgttaa cctgcgcacg agagctgttt
    10261 tcctgcttca gaaaaaactt cgcccaaata tcattgcgtg gcatattttg ctgtaattcc
    10321 ttgaggacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
    10381 ccttccgaac gtatcagaga ggtggcattt aatagcatct ggtcatcaac ttcaccgtgg
    10441 ctaatcagct cagacaaaat catatcagcg ctggtttcat atgctgcaga atctgctttc
    10501 ggattatttt ttcgatgcga taaagcgcga tgccaaattt tttgtttcat cgccgtactc
    10561 agatactgat ttttcgccag attactccac cccgcaggat cgttgaaacg tgtcaataac
    10621 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc cacagcaaaa ataggcagga
    10681 aattttttct ccatcttttt aacaaaatta aactgtaagt tatctggcaa tgctaaaaat
    10741 aacacacgta ttagatcatc gtcattctcg aatttttggt gtaaatatac tgctattttg
    10801 tcactctctt gtggcgtcat ccgcaacaat tgaccatggt gtttcatctc tgccagcttt
    10861 gacgctaagc ttgtgacaac atcgtttgat gaatcgttcg ccaactgcat tgcctgattg
    10921 tagctgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
    10981 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
    11041 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
    11101 agattcaacg gaccgacacc atcaggtaga tgactcattc gcgtcattat ttcgacagag
    11161 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
    11221 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgttcatca
    11281 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
    11341 cgcattatgg caagatcaaa tgtcgcaatg tctcgtcctt caaatgcagc aacgttgcct
    11401 tcatccagca tttgtaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
    11461 tcttttttca tcagattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
    11521 tctttcagag catcttcatc atttattagg ccccaataat atgcatcagg atttattatc
    11581 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
    11641 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatat cccacgcgca
    11701 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
    11761 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
    11821 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
    11881 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
    11941 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
    12001 ctgttgatag cttgttcaac ttgttgttta tcttcagcgg tagccgcaga aaaaaatggt
    12061 tcaatatcaa ctgcatttgc aacacatcga agttccgcct gtactggaag actcagcata
    12121 aacaggggaa gtaaacctat ccagctgcaa atcctgggct taaacatatg cactccatgc
    12181 cattaaaaaa gaaacaaaag ggaaatgcat ctttcccttt tgtatattcg ttattctcgc
    12241 gaaataccat ccaacgtaga caacacttcc tcagaaagat gcaatttttc agcagccata
    12301 ttctcccgta aatgcgcaac agaagacgtc cctgggatca gcaaaatatt cggtgaacgt
    12361 tgtaacagcc acgccagcgc cacctgcatt ggtgttgcac ccaggctcgc agcaacatcg
    12421 gaaagcgtgg aagattgcag cggcgtaaag cccccgagcg gaaagaacgg cacgtaggca
    12481 atgccgtcac gagccaaagc ttcaatcatt gcgtcatcag cacgatgagc aatgttgtat
    12541 tcgttctgca cacagacgat ttcgacaatc ttgcgcgcct ctgcaacctg cgtcggtgtg
    12601 acgttgctta ggccaatatg ttttaccagg ccttgttgtt gcatctctgc cagcacggtc
    12661 aggctggctt ctatcgatcc ttccgctggg ccatgaccat cccccatcat aatgcgcaag
    12721 ttaaccacat ccagtacgtc cagcccgaga ttacgtagat tatcgtgcac cgctttttgc
    12781 agctctgccg gagaaaatgc gggcaaccag gatgcgtcct ctccacgccg cgcaccaatt
    12841 ttagtgacaa ttgtcaggtc gtcagagtaa ggataaagcg cttcgcggat aatctgattg
    12901 gtgacgtgcg gaccataaaa gtcgctggta tcaatgtgat tgacgcccaa tgccagcgcc
    12961 tcacgcagca cggttatagc gacgtggcga tctcttgggg ggccaaaaac cccaggacct
    13021 gccagttgca tcgcgccata accaagacgg ttaacggatt ttgtaccgag agtaaatgta
    13081 ttgctgctca tcgcaccctg ccctgtaata aaacggatta agaaaaatca gtatagaaac
    13141 gaaaagagat aaataatgcg aaggcgtaaa caagattact ggatgagttg ctctgttatc
    13201 cttgaagaca ccaaaacaaa acgcctaaag catcgcatcc ggcaataaaa aaccgcaacc
    13261 tgtatcagca ttgcggtttg tgtatcacat gctaaaaatt agaacgtttt cttgaagttc
    13321 agcatcacgc ctttatcgct gatgccgtcc tctttccagt ggtaagcatc cagattcagc
    13381 gaactttctt tgctgctgta cttcacgccg acgcttgcca ggctgttgat gccgccgccc
    13441 ttctgaccgt catcgacatt aaagtgctga ctgcctgcgc ctgccaggct tgccgtacgc
    13501 tggctcttcg cgtagctcag gttcggcccg ccttccagcg tagcgctagc gctccagccg
    13561 tctttacctg cgtaatccag tttcaacccg acgatgctgt ccaccgccgt ttcgctgcca
    13621 ctgttcatat tcaggttaaa gtctccggca ttgcgctctt gatagccacc ttccagtgtg
    13681 tgacgcagtt taacacccgc atatggcgta accttcagtc cttcgctcgg ttcgaaagtc
    13741 ttcgcccctt cgctgcggaa ctccaggtac tgctgcttca cgtcggtatc agccgttttg
    13801 tttgtgttgc taaatgcaat cgaacggctg ctgtcaaggt tgtgaacgtc ataacgcaat
    13861 gcgttattcc agttcatgcc gttgtcgaag gacatctgat gtttcaggcc aaagaactgg
    13921 ctataaccgc ctgtaacgcc attatcaccc gctttctgcg caccgtcgcc atcgaggcgc
    13981 gcgataccgt actccagact catcgtctgg ctttcgctca ggtcgatggt tttacgcaat
    14041 gccagcatgt cgtactcggt attgttcccc agctccgcgc gcggatcgcc tttcgcaaca
    14101 acgttgaacg ccagaccgtt acccactttc ggtgcggcat ccgccagcat gctaaagcgg
    14161 ttgcttagca cgcgcgcttc gcggaatacc gtggtcgcct ggctaccgct gacctgtttc
    14221 agggcactgt tcagttcggc agtcgtgccg acgttcaggc tggtatacag ctcgttgttg
    14281 gtgtaacccg catccagtgc tttcgcgacg tcattcaccg aagcatcggt cgccacatcg
    14341 gtataagcgt ttttgctcat agtgacgtca acgttgccgc tggcatcggt gctgccttta
    14401 gcgttccaga caacagtaga ggaaacgatg ttatccacac cgctgatgtt ttcgcctttg
    14461 aatacatcgt taacgacaac ggtggtgtcc gcagttcctg cactaaaccc ggcgttaaca
    14521 ctcacgttgt cgccaataac caggttattc gcgctgagcg tacccgaact cccgtcgctg
    14581 ttggtaccaa tggtatagtt gctcagcagt tgcggtgcat ttgaatcgac aggtggcgtt
    14641 ggaacattac cttgtggcgg cgtggttgat gcctctggta cggtaatatc cgcagtaccg
    14701 ttgtgatcgt tttgcgcccc cgtcgttcct ggagcataaa tatcgcatcc ggtgtcacac
    14761 agcagattaa tttcaccgtt gttaatgatg gctttcgttt gcccaccaaa cgcccaggag
    14821 ttatcggcat agacattaat cacacccgtt tgcgtgttat taattgtcgt ttcccgacca
    14881 ttgcccttaa taccaattaa accttcgccg ttggttccat ccagttgccc ctgctcagta
    14941 cccacattaa tgatgccact gttcaccaac tggacaacat agctggttgt gccgccactg
    15001 atgcccactg cattatcgcc ctgaacatta ataacgccag cgttagcaaa gttataacct
    15061 cgtcctaaat caaccaacgg tgcagcagga ttaataccat ttatcactcc tgtttccgta
    15121 ttccaaatcc atttcccgtt actgttcgag ccagtatttt cagttttgat tgcgctatag
    15181 ccatttgtgg ccgtgatggt gccacggtta acctgcgaaa cagaatcact ggttctggtt
    15241 acatgaataa tcgcgttttc cccgcccgtt accgcattac cgctatttgt cagggtcgcg
    15301 ccagcctcat tgaagaacag gaaacttccg ttgacgagtg agtggccatc ttcggtcacc
    15361 gtcatttcat cggtattcac cacattctgt tggtagctgt ctccggtaat tgaaccatta
    15421 aattcgcctt cattaaagaa ggaaaccgcg ccattcaacg tcagcagatc ggttgttatg
    15481 ctccctgttt cacggttata tatcgtggtt ttgctgttaa tgctgtcaac atccaggtga
    15541 ccactgtttt ccaacccctg gtttacgctc agttcgccgt tgattgttag gctggcatca
    15601 ggttggttga atatccagcc atttgctata acgtcgccgt taagcgtttc gtcgccatag
    15661 tttgccaggt tattaactgc gataaaaccg tcgctagttg tagttttact gatgctctcg
    15721 ccgctgccag acagctcact gagaatatct ttgtcatccg gcgtaacgcc aatgtgggaa
    15781 tcactttcat ccattggatt accgaaaaga ttaatttttc catagttgag aatggtgccg
    15841 gtgccatctt tataaaacgc ctggccaata tcagtgttaa tattaataac cccatcctgg
    15901 tcattaatcg ccagaccacc ctgaatggct cccatcgcga acagttggtt atcttgctgc
    15961 ttcgtcacgc cctcatcggc ggtgagattg attgtccctt ggtttacaac ggtcccaccg
    16021 ttcatcgcca gcatgccgaa gccttcattc ttaacgttga tgataccggt attcaccgct
    16081 ttagcatcac cgtcgccggt atccgttgaa cctgccaaca tcccgccccc catcagatat
    16141 acggaattgg tctgccagta agtttcatca ataatattac cgttttcatc tactattgga
    16201 acaaagccgt cgacatttat ctcgcccgca ttggtcgccg ttccttttga agcggaaaca
    16261 ccataggttc ctgcaccatg caaattaatg gttgctcctg tttcattgat taccgatccg
    16321 taattactgg ccaacaaggc ataacccgtg ttgtgatacc atttgatcgg gcgctcgtta
    16381 aatcctccgc tgctaaaagg ccgggtaatg gatgtcagat tgattgttcc tccggaggca
    16441 ttttttgcct gcgagtcatc gcttcttgcc atcattccag caaagaaagt gtgtgattca
    16501 gcatctggcc tgatattaat caccccggca ttgacaactt gcgcccccga attcgccatc
    16561 attaattctt gagcatcggt tgtattaata atgccgttgt tctgaataac agactggttt
    16621 cccgtagcaa caattaacga tgcatcgaaa aaaagatagt cactatctct cactgaactc
    16681 tgaatctcgg ttatcgcgtt ttcatcaaat atgacattcc cgccatcagc caccgaaatc
    16741 agagagaagg atcccaatat ctgtcgggta ttcattacgg aatgaccact tatattgacg
    16801 gtattatttc ctgttacctg cacacctttg atagtatgtc caccactatc accagagata
    16861 ttgataccac catcgagatt gatggtgttg tttgtaccca aaacaaccac gccgtaaaga
    16921 ctgttttcat cccccaggta atccgaatcg acagtaatat tgatgtcgcc ttccagatcg
    16981 acagtattgt tttccccctc gatggtaacg ccggttaacg actggggtga ggtggacggt
    17041 tggtccccca tactggacgt cacattaatg tttcccgtca acagcatact gctgtcgttg
    17101 ctcattatgc tgatgcccgt tccgccctgg aaagtaccgc tgccgtcacg ctcctgaatg
    17161 acatttatat caccgctcaa attcacttgt tcacgatcgc cgctgatcgc aacacccgta
    17221 ccgttcagcg agacatcaat ctcacccacg gtagtaaatt gcgcgtcatc tccggtgata
    17281 acgataccga ttgagtcttg atcttttacg gtcagtttgc cctgattatt gattgtcgtc
    17341 ttactaccgc tgacttcaac gccgtgcccc gcaccgttaa cggtaatatc acctttctgt
    17401 tgcaacgaaa cgcgatcgcc gacaattttc gccccgacag agttttgatc aacgaccata
    17461 tcaccggtga tatcaatggt attatcatca cctgtaatgg tcaccccggt ggactgtttc
    17521 ccggtgacat ccatcatatt ggtggacaac gacagatcat tgtttttacc catcacctcc
    17581 agcccggtgg aaaaatcccc gacatgcatc gccccggcca gggcaatgtc gctggcgtca
    17641 ccgttaattt tcaccccggt cgccctgtcg tcggcattta aatctccaac gttgataaag
    17701 gtactgttat cgccgttgat ctgcacacca ttagagtgat cgtccgctgc acctccgcta
    17761 acgttaatat cacccatatt aatgaatgac gcgcgatcgc cgttcagcag cacgcccgtg
    17821 gaatgcgcat ccaccaccga aatgttgccc ttgttgctga cttcgttatt atcaccggtg
    17881 gcatcaatgc catgcgcgcc accgctgaca ttgatcccgc cttccattct gattctggtt
    17941 gcgtttcctg caacttttac cgataccgag ttgtcgccat caatatcgct ttgccccatg
    18001 ttgttgataa ccgcgttgtc acccgtcaca atgacacccg tgccgccgtt agtgatcact
    18061 gcatcaccgc ggttattcag gattgcacga tcgccgttaa ccacgcccgc tgtgccgcca
    18121 tcgacaacca tatggccgtt attgttaacg gtaatatcgt ttccggttat cgccacaacc
    18181 acagaatctt ttcccgtagc agaaatatca ccgtcgttat taagggcgat cctgctgccg
    18241 tttatttcaa tcaccgtcgc gccattcagc gcggcattac cgccctggtc ggtgagcacc
    18301 gcatcgctga ggttaaatac gttgctgttc gcggggtcat ccaccgttgc gccgacggtt
    18361 ttggtgatat agataatgcc gtctttaccg taacaccacg tcataccgcc aaggttattc
    18421 acgccggtaa tttctaccgt attggcatcg gtgtcgacat cccagccctg cacaatagag
    18481 gttcggccat cttcagccgt cagggtgtaa cttccatcac tgtttttgct gtaggtaaag
    18541 gtggcattgc gaatttgcag cgtgcctttg tcttcgtcga atgtgacact gttattgaag
    18601 attttgggct cttttggtgg agtgacgtcg ccaccgtcat ctggaggaac aggtgtatca
    18661 ccaccgtcgt ctggtggaac tggtgtatcg ccaccatcgt ctggtggaac tggtgtatcg
    18721 ccaccgtcat ctggaggaac aggtgtatca ccaccgtcat ctggtggaac aggtgtatca
    18781 ccaccgtcat ctggtggaac aggtgtatca ccaccgtcat ctggtggaac aggtgtatca
    18841 ccaccgtcat ctggtggaac aggtgtatca ccaccgtcat ctggtggaac aggtgtatca
    18901 ccaccgtcat ctggttctga actgtcatga tgatcaccat cattaagcgc catcaccgcg
    18961 gctcctgtaa cgactgcaac caggccagct gcagtccagc tcattacggc gctgtcatca
    19021 gaatcccgac attctgatgg taatttagct aactgctctt tgctaataga atgaatattc
    19081 gccggacatg tgatcttgct ggatttccgg gtgtcatcag ttatatccag gatcgatgta
    19141 tcagttgccc atgtctgacc gctcaatgcc atcgccacac acacagacaa caattttttc
    19201 ctttgcattt atatttcctt agaaatagag cgttgcacgc cagtcgacaa aaataaaagc
    19261 gggccttttt gacggaacgc aagaccatga ttggtgttta tattcacgaa agaaatataa
    19321 ataaagaacc aatcaaaaat atgtgatgtt ctaataattt atactatccg ttgaagtcaa
    19381 ttcaacttaa atgatattta at
//
LOCUS       DAYPHN010000088        19292 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000088 DAYPHN010000000
VERSION     DAYPHN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.088"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            30..914
                     /gene="yeaD"
                     /locus_tag="ACG0BW_002906"
     CDS             30..914
                     /gene="yeaD"
                     /locus_tag="ACG0BW_002906"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFK7259842.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAYFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(965..1819)
                     /gene="yeaE"
                     /locus_tag="ACG0BW_002907"
     CDS             complement(965..1819)
                     /gene="yeaE"
                     /locus_tag="ACG0BW_002907"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFK7259843.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLVAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSPAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1909..2655)
                     /gene="mipA"
                     /locus_tag="ACG0BW_002908"
     CDS             complement(1909..2655)
                     /gene="mipA"
                     /locus_tag="ACG0BW_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFK7259844.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3091..5025
                     /gene="yeaG"
                     /locus_tag="ACG0BW_002909"
     CDS             3091..5025
                     /gene="yeaG"
                     /locus_tag="ACG0BW_002909"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFK7259845.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5138..6421
                     /gene="yeaH"
                     /locus_tag="ACG0BW_002910"
     CDS             5138..6421
                     /gene="yeaH"
                     /locus_tag="ACG0BW_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFK7259846.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6568..8043
                     /gene="cdgI"
                     /locus_tag="ACG0BW_002911"
     CDS             6568..8043
                     /gene="cdgI"
                     /locus_tag="ACG0BW_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFK7259847.1"
                     /translation="MIRSTRVSLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8224..9714
                     /gene="dgcJ"
                     /locus_tag="ACG0BW_002912"
     CDS             8224..9714
                     /gene="dgcJ"
                     /locus_tag="ACG0BW_002912"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFK7259848.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRTICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            9757..10260
                     /gene="yeaK"
                     /locus_tag="ACG0BW_002913"
     CDS             9757..10260
                     /gene="yeaK"
                     /locus_tag="ACG0BW_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFK7259849.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(10261..10365)
                     /gene="yoaI"
                     /locus_tag="ACG0BW_002914"
     CDS             complement(10261..10365)
                     /gene="yoaI"
                     /locus_tag="ACG0BW_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFK7259850.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            10535..10981
                     /gene="yeaL"
                     /locus_tag="ACG0BW_002915"
     CDS             10535..10981
                     /gene="yeaL"
                     /locus_tag="ACG0BW_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFK7259851.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(10938..11759)
                     /gene="nimR"
                     /locus_tag="ACG0BW_002916"
     CDS             complement(10938..11759)
                     /gene="nimR"
                     /locus_tag="ACG0BW_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFK7259852.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTSTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            11856..13037
                     /gene="nimT"
                     /locus_tag="ACG0BW_002917"
     CDS             11856..13037
                     /gene="nimT"
                     /locus_tag="ACG0BW_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFK7259853.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDTNGDWSIPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            13092..13439
                     /gene="yeaO"
                     /locus_tag="ACG0BW_002918"
     CDS             13092..13439
                     /gene="yeaO"
                     /locus_tag="ACG0BW_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFK7259854.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(13461..13715)
                     /gene="yoaF"
                     /locus_tag="ACG0BW_002919"
     CDS             complement(13461..13715)
                     /gene="yoaF"
                     /locus_tag="ACG0BW_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFK7259855.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTTQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            13898..14923
                     /gene="dgcP"
                     /locus_tag="ACG0BW_002920"
     CDS             13898..14923
                     /gene="dgcP"
                     /locus_tag="ACG0BW_002920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFK7259856.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            14956..15054
                     /gene="yoaK"
                     /locus_tag="ACG0BW_002921"
     CDS             14956..15054
                     /gene="yoaK"
                     /locus_tag="ACG0BW_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFK7259857.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            15057..15131
                     /gene="yoaJ"
                     /locus_tag="ACG0BW_002922"
     CDS             15057..15131
                     /gene="yoaJ"
                     /locus_tag="ACG0BW_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFK7259858.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(15190..15438)
                     /gene="yeaQ"
                     /locus_tag="ACG0BW_002923"
     CDS             complement(15190..15438)
                     /gene="yeaQ"
                     /locus_tag="ACG0BW_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFK7259859.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(15586..15768)
                     /gene="yoaG"
                     /locus_tag="ACG0BW_002924"
     CDS             complement(15586..15768)
                     /gene="yoaG"
                     /locus_tag="ACG0BW_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFK7259860.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(15772..16131)
                     /gene="yeaR"
                     /locus_tag="ACG0BW_002925"
     CDS             complement(15772..16131)
                     /gene="yeaR"
                     /locus_tag="ACG0BW_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFK7259861.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(16304..16942)
                     /gene="leuE"
                     /locus_tag="ACG0BW_002926"
     CDS             complement(16304..16942)
                     /gene="leuE"
                     /locus_tag="ACG0BW_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFK7259862.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(17069..17992)
                     /gene="dmlR"
                     /locus_tag="ACG0BW_002927"
     CDS             complement(17069..17992)
                     /gene="dmlR"
                     /locus_tag="ACG0BW_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFK7259863.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            18095..19180
                     /gene="dmlA"
                     /locus_tag="ACG0BW_002928"
     CDS             18095..19180
                     /gene="dmlA"
                     /locus_tag="ACG0BW_002928"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFK7259864.1"
                     /translation="MMKTMRIAAIPGDGIGREVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
BASE COUNT         4913 a         4704 c         4589 g         5086 t
ORIGIN      
        1 ctgataaaat gaagttaaag gactgcgtca tgattaagaa aatttttgcc cttccggtca
       61 tcgaacaaat ctcccctgtc ctctcccgtc gtaaactgga tgaactggac ctcattgtgg
      121 tcgatcatcc ccaggtaaaa gcctcttttg cattacaggg cgcacacctt ctctcatgga
      181 aacctgcggg tgaagaagaa gttctgtggt tgagtaacaa cacaccgttc aaaaatggcg
      241 tcgctattcg cggtggcgta ccggtttgct ggccgtggtt tggtccggct gcacaacaag
      301 gcctgcctgc gcacggtttt gcccgcaacc tgccgtggac gctgaaatca catcatgaag
      361 atgctgatgg cgtagcgctg acttttgaat tgacgcaaag cgaagagacg aaaaaattct
      421 ggccgcacga ctttacgctg ttagcgtatt tccgcgtggg taaaacctgt gaaatcgatc
      481 ttgaagcaca cggcgaattt gaaaccacct ctgccctgca tacctacttt aacgtgggtg
      541 atatcgctaa ggtaagcgtc agtgggctgg gcgatcgctt cattgataaa gtgaatgacg
      601 cgaaagaaga tgtactgacc gacggtattc agaccttccc tgaccgtacc gatcgcgtgt
      661 atctgaatcc gcaagattgc agcgtgatta atgatgaagc gctgaatcgt attatcgccg
      721 taggtcacca gcatcatctg aacgttgtag gctggaatcc gggaccggcg ctttcagtta
      781 gcatgggcga catgccggac gatggctaca agacctttgt ttgtgtagaa acggcttacg
      841 cttcagaaac gcaaaaagtc accaaagaaa aacctgcaca tctggcgcaa tccattcgcg
      901 ttgccaaacg ttaatttgcg ttaatgttgt gtgccgggtg cgatgcatcc ggcacgtatc
      961 aacatcacac catatccagc gcagtttttc cttttggtgc cggatacgct ttatccagca
     1021 tagctaattc cgctggagaa agttcgacct caagcacagc cgcattctgt tggacatggg
     1081 caatcgtggc cgcttttgga atcgccatca caccctgatg gctgattacc cacgccaaca
     1141 atacttgtgc cgagctgata ttgtgagcat gtgcaatttc gttgactact gcgtttttta
     1201 gcagcccatt gcgcaaccgc ccggcctgag ctaacggact gtaagccatc accggtatct
     1261 gctgttgctg gcaccaaggg agtagatcgt actcaatccc tcgtgaaccg agatggtaaa
     1321 gtacctgatt agtggcacac tgatttcccc ccggcagctg ccagagttcc tgcatatcgg
     1381 cataatcaag gttagaaacg ccccagcggc ggattttgcc ctgggcgacc aatttttcca
     1441 tcgctgcgac agtctcttcg agagcgaaac tgccagacca gtgtaataag tagagatcga
     1501 gataatcagt attgagacgg cgtaaactgg cttcgcatgc atttatcgct ttttgcccgc
     1561 cagcattcca cggatagact ttagagacga gaaagacctt ctctcgcaga ccggttaatg
     1621 cttccccaac caccttttcg gcaccaccat cggcatacat ttcggcggta tcaatgaggg
     1681 ttaaaccgag ttcaatgccc gcgcgtagtg cagctacttc tgttttgcgc tgactggcat
     1741 cttcgcccat ataccatgtt ccctgcccta tggctggcag tgagacatcg ccactaaatt
     1801 gaatcatttt ttgttgcatt gtttcctccc aggtattgca ccaccgtaat gcaaaacagg
     1861 gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag atgcattatc agaatttgta
     1921 ggtgatcccg gtagaaatca ggccagtcca ggatttatcc accattgggc tgtcagtaac
     1981 ttcatcagac agacgggtgt agcgcgcggt accgtaaaca ctccagtcgc cgaggaagtt
     2041 gtagctggcg ctcagctcca ggtaagggct ccagctgtcg ttcgggttat agccacgcag
     2101 accgctgcga gcggactctt tgcgcgatac gccataatag tattcgttct ggttttcgct
     2161 gttccactgc acaccaatac ccggagtcac ggtcaggcca ccgttggtgt aacgatacaa
     2221 ccaggccata tcccagacga tgccgttgct gttatccagg gtatcgccag ccagggtggt
     2281 acgcaggtaa ccgtactggg taaagtgagc ataagacaga ccagccatca tggtgctctt
     2341 acggtcatcc aggtgacgca tttggtgatc gccactgtct ttcgctttga agtaaagcgg
     2401 cgaccaataa gcggtaattg aaagtttgtc cgttgcgtca ttccacaggt agtaaccacc
     2461 acctaagcca cggaaccaga agttatcgcc ttcatagttg attaccggta ctgggtagac
     2521 atcggtatcg taatctttat atgggtgttc aacgacacct acgcctgcgc ccagggaaaa
     2581 tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata agcactccaa gtgccagaag
     2641 tttgagtttg gtcacaatta atcattcctt aaacaaatgt ttagcgggcg acaaagttta
     2701 cccgtcaata catcgaaacc caaccttttt acgttttcat tttttaaagt aactgtttaa
     2761 ttttcctgac gcggatgaca ccgcgcttac agccaaatga atttagcgtt actggcgagc
     2821 ctggtcttta cattaattat gcaaaattta tggatgagtt gttgatatgc cattgaaatt
     2881 aagaaagccg tgcaggcaag ttttccattt gccatctacg cttaattttg aaggtgtatc
     2941 accgggcacg ttgttctcat cgtcgataaa atggcatgag agttgctgtg ttttagcaag
     3001 agacgtcgtt cagtttacct cttccgggag cctctactat tcatatgaac ggctcttaac
     3061 ctgtgctaaa aaacgaaagg acggcatacc atgaatatat tcgatcacta tcgccagcga
     3121 tatgaagctg ccaaggacga agagttcaca ctgcaggagt ttcttaccac ttgtcggcaa
     3181 gatcgcagtg cttatgccaa cgcggctgag cggctattga tggctatcgg tgagcctgtc
     3241 atggtcgata cagcccagga acccagactt tctcgactct tttctaaccg ggtcattgca
     3301 cgttatccgg cgtttgaaga gttttacggc atggaagacg cgattgaaca gattgtctct
     3361 tatctgaaac acgcggctca ggggctggaa gagaagaaac aaattctgta tctgctgggg
     3421 cctgtgggtg ggggtaaatc atcgcttgct gagcgactga aatcattaat gcagctcgta
     3481 ccgatttatg tattgagcgc caacggtgag cgtagcccgg tcaacgatca tccgttctgt
     3541 cttttcaatc cgcaggaaga tgcgcagatt ctggaaaaag agtatggcat tcctcgccgt
     3601 tatctcggca ccatcatgtc gccgtgggcg gcaaaacgcc tgcatgaatt tggtggcgat
     3661 atcactaagt tccgggtagt gaaggtctgg ccgtcaattc tgcaacaaat tgctatcgcc
     3721 aaaacggaac ccggtgatga gaacaaccag gacatctccg cgctggttgg gaaagtcgat
     3781 attcgtaaac tcgaacacta cgcgcagaat gacccggacg cctacggcta ttccggtgca
     3841 ctgtgccgcg ccaaccaggg gatcatggaa tttgtcgaga tgtttaaagc accgattaaa
     3901 gtgctgcatc ccttgctaac cgccactcag gaaggtaact acaacgggac ggaaggtatc
     3961 tccgccctgc cgttcaacgg gattattctc gcccactcga acgaatccga atgggtcact
     4021 ttccgtaata acaaaaacaa cgaagccttc ctcgatcgtg tttacatcgt gaaggtgccg
     4081 tattgcttgc gcatttccga agagatcaaa atctacgaga aattgcttaa tcacagtgaa
     4141 ttgactcacg ccccctgcgc ccctggcacg ctggaaacgc tgtcacgttt ttccattctt
     4201 tcgcgcctta aagagccaga aaactccagc atttattcaa agatgcgggt ttatgatggc
     4261 gaaagtctga aagacacaga tcccaaagcc aagtcgtatc aggaatatcg tgactacgcc
     4321 ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg cgtttaagat cctctcccgc
     4381 gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg tccatctgtt ctacgtcctg
     4441 gaacagcaga tcgagcgcga gcagttcccg caagagcagg cagaacgcta tctggagttc
     4501 ctgaaaggtt acttgatccc gaaatatgcc gagtttatcg gcaaagagat ccagacggcc
     4561 taccttgaat cctattccga atatgggcaa aacattttcg accgttatgt tacctacgcg
     4621 gatttctgga ttcaggatca ggagtatcgc gatccggata ccgggcagtt gtttgaccgc
     4681 gagtctctta acgccgagct ggagaaaatc gagaaaccgg cggggatcag taatccaaaa
     4741 gatttccgca acgagattgt taacttcgta ctgcgcgcca gagcgaataa cagcggacgc
     4801 aatccgaact ggaccagcta tgaaaaactg cgcacggtta ttgagaagaa aatgttctcc
     4861 aataccgagg agctgttgcc ggttatttcg tttaacgcca aaacgtcaac cgacgagcaa
     4921 aagaaacacg acgattttgt cgaccgtatg atggaaaaag gctacacccg taaacaggtg
     4981 cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt cttaacaacc ctggcccggt
     5041 acacatgcta ccgggcctac aacgacagcg aaccgtgggc cagagaagcg gcaacacagg
     5101 cgtagcatac agttggcaaa tgtagtacgg ggggcatatg acctggttta ttgaccggcg
     5161 tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt tttttacgcc gttataaagc
     5221 gcaaattaaa cagtcgatct ccgaggccat taataagcgt tcggtgactg acgtcgacag
     5281 cggcgagtct gtatccattc ccacggaaga tattagcgaa ccgatgtttc atcaggggcg
     5341 tggcggtctg cgccaccgcg tgcatccggg caatgaccat ttcgtccaga atgaccgaat
     5401 tgaacgtccc cagggtggcg gcggaggttc cggcagtggt cagggccagg ccagccagga
     5461 tggtgaaggt caggatgaat ttgtctttca gatttcgaaa gatgagtatc ttgatctgct
     5521 ctttgaagat ctggccttac cgaatctgaa acaaaaccaa caacgccagc tgaccgaata
     5581 taaaacgcat cgtgcgggtt ataccgctaa cggcgttccg gccaatatca gtgttgtgcg
     5641 ttcattgcag aactcactgg cacgacgcac agccatgacg gcgggcaagc ggcgggaact
     5701 tcatgcgctg gaagagaatt tggccatcat cagcaatagt gaacctgcgc aactgctgga
     5761 agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc aaaattgaac gcgtcccttt
     5821 cattgacacc ttcgatttac gttacaagaa ctacgagaag cggcccgatc cctccagcca
     5881 ggcagtgatg ttttgcctga tggacgtttc cggttcaatg gatcagtcca ctaaagatat
     5941 ggctaagcgt ttttatattc tgctgtatct gttccttagc agaacgtata agaacgtgga
     6001 agtcgtatac atccgccatc atacccaggc gaaagaagtc gatgaacatg agtttttcta
     6061 ctcgcaggaa actggcggca ccattgtttc cagcgccctg aaactgatgg atgaggtagt
     6121 gaaagagcgt tataacccgg cacagtggaa tatttacgcg gcacaagcat cggacggcga
     6181 taactgggcc gatgactctc cgctttgcca tgaaatcctg gcgaaaaaat tattacccgt
     6241 cgttcgttat tacagctata tcgaaattac ccgtcgtgca catcagacac tgtggcgaga
     6301 atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg cagcatatcc gcgaccagga
     6361 tgatatttat ccggtgttcc gtgaactgtt tcataaacaa aatgcaacag ctaaagacta
     6421 aaactatcag ccgggtcatt atcgcctggc tgatttttag ctcactgtaa attatctcat
     6481 ttattacata cattagctta caatcgcttt aaatatgaca gcataacctt tacacaattt
     6541 agttccagaa aacaatcatt cggaaaaatg attcggtcaa cacgtgtttc cctggggtta
     6601 ttctttaaat attttttatc gttaacgaaa attgatcctg gtcaaaacta tatatctctg
     6661 ccatcaataa aatccagcac tcacattgct ctccttttta tggcttctat gggtacacaa
     6721 aaattaaaag ctcaaggctt ttttattttc agcttattgc tgacgttaat tttattttgc
     6781 attactacct tatataacga aaacacgaat gtaaaactca tcccacagat gaattacctg
     6841 atggttgttg tggctttgtt tttccttaac gccgtcattt ttcttttcat gttaatgaag
     6901 tatttcacta acaaacaaat tttaccaaca ctcattttaa gccttgcatt tttaagtggc
     6961 cttatctatt tagttgaaac cattgtaatt atccataaac caatcaacgg cagtacactg
     7021 atccagacaa agtcgaatga tgtttctatt ttctatattt tccgccaact cagtttcatt
     7081 tgtttaacct cgctggcgct cttttgttat gggaaagaca acatccttga caacaataag
     7141 aaaaaaactg gcattctgtt actggcactg attccttttt tagtttttcc ccttctggca
     7201 cacaatctga gcagttataa cgctgactat tctttgtatg tcgtcgatta ctgtccggac
     7261 aaccatactg cgacctgggg aatcaactat acaaaaatat tggtttgtct gtgggcattt
     7321 ttactgttct ttattatcat gcgcacacga ttagccagcg aactatggcc gttaatagca
     7381 ttattatgtc tggcatcgct atgctgcaac ttacttctac taactctgga tgagtataat
     7441 tacaccatct ggtatatcag tcgcgggatt gaagtctcca gtaaactgtt tgttgtgtct
     7501 tttctgattt ataacatttt tcaggaactg caactctcca gcaaactggc agttcatgat
     7561 gtgctgacca atatttataa tcgccgctac tttttcaaca gcgtagagtc attattgtcg
     7621 cgacctgttg ttaaggactt ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc
     7681 aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg tttcaattgt cgatattatc
     7741 cagcaaagca tccgccccga tgatatttta gcgcgactgg agggtgaggt gtttggcttg
     7801 ctatttaccg aactgaatag tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat
     7861 gtcgaactcc tgaccggctt tagtaacaga tatgatgttc ctgagcaaat gaccatcagt
     7921 attggcacgg ttttttcaac gggtgacaca cgtaatatct cgcttgtcat gacggaagca
     7981 gataaagcct tacgcgaagc gaaaagcgag gggggcaaca aagtgattat tcatcatatt
     8041 taagtgcaaa aatattcaga gccatgcttt ttgcgtggct tttgcataca atttattact
     8101 attaccccta aatttcccct ccccagcggc gcagagatga gtataattag cgcccctgtg
     8161 ccaggccgca atcgaacttt atctggtttt ctcgtttcac taaccgaagg agtgccattt
     8221 atcatgaaat tgcaccatag aatgctccgg cattttatcg ccgcaagtgt cattgtgctg
     8281 acatcttcct tccttatttt tgaacttgtc gccagtgaca gagcaatgag tgcctatctg
     8341 cgctatatcg tgcagagagc agactcctcc tttctttatg ataagtatca gaatcagagt
     8401 attgccgcgc atgtgatgcg cgctctcgct gctgagcagt cggaagtgtc gccagaacag
     8461 cggcgcacca tctgcgaggc ttttgagtct gccaataata cccatggctt aaacctgact
     8521 gcccataaat accctggctt acgcggcaca ctacaaaccg catccactga ctgcgacaca
     8581 attgtggaag ctgcagcact attacccgct tttgatcagg cagtggaagg caaccgccac
     8641 caggatgatt acggttcagg tcttgggatg gccgaagaga aatttcacta ttatctcgat
     8701 ctcaatgacc gctatgtcta tttttatgag ccggttaatg ttgaatactt tgcgatgaat
     8761 aactggtctt tcctgcagtc aggaagtatt ggcatcgatc gcaaagatat tgaaaaggta
     8821 tttaccgggc gtaccgtttt atcgagcatt taccaggatc agcgtactaa acagaacgtg
     8881 atgagtttgc tgacgccggt atatgtcgca gggcagctaa aagggattgt gctgctggat
     8941 attaacaaaa acaatctgcg gaatatcttt tacactcatg accgccctct cctctggcgt
     9001 tttctcaatg tcacgctaac cgataccgat tcggggcgcg acattatcat caaccagagc
     9061 gaagacaatc tgttccagta tgtcagttac gtccatgact taccgggcgg cattcgtgtc
     9121 tcgttatcca ttgatattct ttactttatc acgtcttcgt ggaaaagcgt tctgttctgg
     9181 attttgacgg cgttaatttt gctgaatatg gtgcggatgc acttccgttt ataccaaaat
     9241 gtgtcgcgag aaaatattag tgatgcgatg actggactct ataatcgcaa aattttaacc
     9301 cctgaactgg agcagcggtt gcaaaaactg gtgcaatccg gttcttcggt gatgtttatt
     9361 gcaattgaca tggacaagtt aaagcaaata aatgacaccc tcggtcatca ggagggggat
     9421 ttagcgatta cgttattagc gcaggcgatt aaacagtcaa ttcgtaaaag tgattatgcc
     9481 atccgactcg gtggcgatga attctgcatc attcttgtcg attcgacgcc gcaaattgca
     9541 gcacaactgc ctgaacgtat cgaaaaacgt ctgcaacata tcgcgccgca gaaagagatc
     9601 ggcttctctt ccggtattta cgcgatgaaa gaaaacgata cgttacatga tgcgtataaa
     9661 gcttccgatg agcgtttata tgtcaataag cagaacaaaa acagccgttc atgataacct
     9721 tctatggttg tttgcttgta atctcaggag catgaaatga ctgaaatggc taaaggaagc
     9781 gtgacgcatc agcggttaat cgctttatta tcacaagaag gtgctgactt ccgcgtcgtt
     9841 acccatgaag cggtaggtaa atgtgaagcg gtgtcagaaa ttcgtggcac cgctttaggc
     9901 cagggggcta aggcactggt atgtaaagtc aaaggcaatg gtgtaaatca acatgttctg
     9961 gcgatcctcg cggccgatca acaggccgat ctgagccaac tcgccagcca tataggcgga
    10021 ttacgcgctt cgttggccag cccggcggaa gtcgatgaac tcacaggctg tgtcttcggc
    10081 gcgatccccc ctttcagctt ccatccaaaa ctcaaactgg ttgccgatcc cctactcttc
    10141 gaacgatttg atgaaatcgc cttcaatgca ggcatgctgg ataaatccgt tattttgaaa
    10201 accgcggatt atttgcgcat tgcgcaacca gaactggtca atttccgccg cactgcgtaa
    10261 ctagcctgtc cgttcgataa gaagaacgga caaaaccagc acaacagcaa tggcaaaaaa
    10321 cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagtat gcccccggga
    10381 gatagctgtt ttcacgttat tgttagcgtg cacaaattgc agtttgatga cagttcgcct
    10441 gattttttat gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa gcgtaaagta
    10501 agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt tgctgatcct
    10561 gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg tttcaattct
    10621 ggtgttaatc attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga ttgaaaaaca
    10681 ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca tcgccagcgg
    10741 gacgctaccg ccctcaaccc tgattcactc gtttctgaac tggaaatcgc tggtggcaat
    10801 tgcagtaggc gttattgtct cctggctagg tgggcgcggc gtgacgttaa tgggcagcca
    10861 gccgcaactg gtcgccgggc tgctggtggg cactgttttg ggcgtggcgc tgtttcgcgg
    10921 cgtaccggtt ggtccgctta ttgccgcggg tctggtttcg ctgattgtgg ggaaacagta
    10981 gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa catagtaata
    11041 aaggccgttg ttgaatcata ccccagcgta tgagcaactt tctgtacagt atcgcctttc
    11101 actaatccct gaagcgccat aatcagctgt aactgctgac gccactggcg aaaactcaac
    11161 cccgtctctt tcactatcag tcgggcaaga ttgcgttcac tcatcgcaaa aaagcgagcc
    11221 cattgcccca atgaccccca ctcgacgggc tctttcgcca tcatctccac catcgcgcga
    11281 attttaggat gagaagaaac gggtaggtgc agctgttgtt gtggctgctg agggagttca
    11341 tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt cgacgtcctg
    11401 tttgccaggg ttaaaattaa ttcccggcat aagggcgata ttttcaatgt gcaacaggtt
    11461 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
    11521 acttgattgc tatgttccac cccgcccggt atccaaaccg catattgcgg tggcaccatc
    11581 cacaaagcat tttccaccgt acaggtaatt gcaccatgta gcgccagtat cagctgtcct
    11641 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
    11701 accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct gtgcatcatt
    11761 ttgtccgaat ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg
    11821 taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg
    11881 gcaaaaacag gattgtcctt atcgctggca ttttaatgat tgccacaaca ttacgcgtca
    11941 cctttaccgg cgcagcaccg ttactggata cgattcgttc cgcttactcg ctgacgacgg
    12001 cgcaaaccgg cttattgacc accctgccat tattagcctt tgcgctaatc tcacctctgg
    12061 ctgccccggt ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga
    12121 tctgtgctgg tatcgcaatt cgctctctcc cttcgcctta cttattattt ggcggtacag
    12181 cggtcattgg cggtggaatt gcattaggca atgtcttact gccaggatta attaaacgcg
    12241 atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag
    12301 cggcactggg atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg
    12361 cgttgctcat gctgatgtct tttcctctgc tggctctttt tttatggctg ccacagtggc
    12421 gaagtcaaca acatgcaaat ttgagtacct cacgcgcctt acatactcgg ggtatctggc
    12481 gttctccgct tgcctggcag gtaacattgt ttcttgggat caactcactg gtctattacg
    12541 tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg
    12601 gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg atcccacttt
    12661 tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag
    12721 tgggcgcggt tgggctctgc tttatgccag cgcacgcgat cacctggact ctgcttttcg
    12781 gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt cgggcaagtt
    12841 ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat ttgctggcag
    12901 cctgtgggcc gccgctgatg ggtaaaatac acgatactaa cggtgactgg tccataccat
    12961 tgatgggtgt tgccatactt tcactactga tggcgatttt cggactttgc gccgggagag
    13021 acaaagaaat tcgctaatat ccggtgctat agtgacgtga ccaatcatgc gtggaaggga
    13081 gaacaaacac gatgaatatt cagtgcaaac gcgtttatga tccggcagaa cagagcgatg
    13141 gttatcgcgt actggtcgac cgcctctggc cgcgtggtat caaaaaaacc gatttagccc
    13201 ttgatgagtg ggataaagaa atcacgccgt caacggaact gcgcaaagcc tttcacggcg
    13261 aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg caacatgagc
    13321 aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc gctgaccctg ctctactcag
    13381 caaaaaacac cacgcagaac catgcgctgg tgctggccga ctggctacgt agcttgtgat
    13441 tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg ccacaatgcc
    13501 cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc ttgcgttgtc
    13561 tgcactggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg atttgccata
    13621 ccaatctgcg gcggtttggg tgcttctggc tgagaagggg ttgaacaacc agccaggacc
    13681 agcaagcaag gcagaacaaa actgataatt ttcatttatt gatctcacat atttatccag
    13741 acttagagta tcgcggtatc gttttgtttt gcagcactat ttttattaca ttcactcaaa
    13801 tcatattacg ccttgtttca tctttgttga tgatgtttta tcatgcctgc aaagattaaa
    13861 taatcagcat ttacccgccg tatcctggag ttgtttcgtg tcagatcaga ttatcgcccg
    13921 cgtctcacaa tcccttgcca aagaacagtc actggaaagt ctggtccgac agcttctgga
    13981 gatgctggaa atggtcactg atatggaatc aacctacctg accaaagtgg atgtcgaagc
    14041 gcgcctgcag catattatgt ttgcccgtaa cagccagaaa atgcacatcc cggagaattt
    14101 taccgtctcg tgggattact cgttatgcaa acgcgccatt gatgaaaact gctttttcag
    14161 cgatgaagtc cctgaccgtt ggggcgactg tattgcggca cgcaatcttg gcatcaccac
    14221 atttctgagc acgccaattc acttaccgga tggatcattc tatggcacgc tttgcgccgc
    14281 cagtagtgag aagcgccagt ggagtgaacg cgcggaacag gttttacagt tattcgccgg
    14341 actgattgca caatatattc aaaaagaggc actggttgaa cagctgcgcg aagccaatgc
    14401 tgcgctgatt gcgcaatcgt ataccgactc gttaaccggg ctaccgaatc ggcgggcgat
    14461 ttttgaaaac ctgacgacgc tgttttctct cgcccgacat cttaaccata agataatgat
    14521 cgcgtttatc gatctggata acttcaaatt aatcaacgat cgttttggtc ataatagtgg
    14581 tgatttgttc ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa atggcgaagt
    14641 tattggtcgt ctcggcggtg atgagttttt ggttgtttca ctgaacaacg agaatgcgga
    14701 tatttcgtct ctgcgagaac gtattcagca gcaaatacgc ggagaatatc acttaggtga
    14761 tgttgatttg tattatcccg gtgccagtct tggcatagta gaagtcgatc ctgaaacgac
    14821 cgatgcagac agtgccctgc atgctgccga tatcgcgatg tatcaggaga aaaaacacaa
    14881 acagaaaaca ccttttgtcg cgcatccagc gctacattcc tgaggcgtat tcacatcctt
    14941 ttgattggtg ataacatgcg aatcggtatt atttttccgg ttgtaatctt tattacagcg
    15001 gtcgtatttt tagcatggtt ttttattggc ggctatgctg ccccgggagc ataaagatga
    15061 aaaaaacaac gattattatg atgggtgtgg cgattattgt cgtactcggc actgagctgg
    15121 gatggtggta acgtcacatc tagaaaatag caaaggctgc ctgtgtgcag cctttgtgca
    15181 atttacgcgt taacttttaa tctttctgta gataaacagc acgacaatcg cgccaataac
    15241 ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa acagcgtgct
    15301 gatccatccg ccgactacgg caccgactat ccccagcagg atagtcataa agaatccacc
    15361 accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc caaaaataat
    15421 ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg gttagtcgct
    15481 gataaaaagc atagcacaac atcgggaggg caagatttgt gacgagcatc acggaggatt
    15541 tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc aaccacaaac
    15601 atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt ttatcacttc
    15661 agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat cgacagaaac
    15721 gccattgctg ttattggtga cggtcacggt atacgttgct ttgcccatga ttcatttccc
    15781 gttatgaatg acttttcgtt gttgcgcgcc ttccatcagg acttcagggg caacgaagaa
    15841 gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact tttctggagg
    15901 gaacaccgca aactgccccg cttcgataag gattacttga tcaggctctg tactgtgttc
    15961 atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg ggtaaacccc
    16021 cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag tttgtttatt
    16081 ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca tccttttccc
    16141 tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac gcctgataat
    16201 ttgcatttta aataccattt attggttact ttttagcacc gtatcagaga agaatcaggg
    16261 aggattacag gtgggaagcc cgcgacaacg cgggctgaat gcatcaggac tggagcgtcg
    16321 ccagtcgggc ggcgaaaccc acaaacatca aaccaatcag tgagttgccc actttagcca
    16381 gtttcttttt ggtacgtata tactgcgtga caaaagcacc agaaataatc aggaagctca
    16441 aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa attcccgtat
    16501 gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa atggctttcg
    16561 gattagtcag gctcaaaatt aacgcgcgtt taaaaatggc accgtattgg ggctcatcgg
    16621 atttggcctc gttattttta cccttcaggg tcgcgtagag aattttactc cccagataga
    16681 gcaaataaaa tgcaccaaga taacgtacaa tgttgaataa tatcggggtg gtcttaatta
    16741 atgtcgccac tccagcccat gccagaaaca tcaataccgc atcgccaata aatacaccgc
    16801 aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt acaaacaggg
    16861 tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc cagtaattca
    16921 gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca tgcgcgatta
    16981 aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag gttttaaccc
    17041 gatatcaacc cgataattga atcattaatc aggcatgctc cagtgaaaaa ttcgggtagt
    17101 gctcggcaaa atactggcgt aaaaattcta ccgttatccg cactttcgct gacgtcgcca
    17161 gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt agcacttgta
    17221 ctaaatgacc actggcaatg ttttcgctaa catcccacca ggagcgcaga gcaatcccct
    17281 gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt gggccggtaa
    17341 ctttaatcgc atggtggcct tctttattgc gtaattgcca gacaccaaaa ggatgatcgc
    17401 gctctttaat gaccaggcac ggcaatgcag aaagatcggt taaatgtttc ggcgcgccat
    17461 gttgcgcaat aaattccggc gacgcgcaga gaatgcggta attggtcgcc agtttgcggg
    17521 cgattaaatt aggggcgata tcatcgccaa tgcgaatatc gagatcgaca ccttcattga
    17581 ccaaatcgac caaccggtct tccacatcaa aacgtaattc cagttgcgga tacgccttcg
    17641 ccagcgctga taacgccgga gccactacct gtcgtccaaa accaaagctg ctgataatac
    17701 gcagcatccc ctgcggcacc tgacgcacgt cagaaagttc gtccatcatt tgaccgacat
    17761 cctgcaaaat ccgctgcgcc cattcataaa tccgctctcc ttcttcggta atggtgacgc
    17821 ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag gcgatgcgct
    17881 tgctgacgaa cgccggagaa acgcccagtt cttccgccac ggcggcaaaa ccagcccggc
    17941 gagccaccag cataaagacg cgcaaatcgt tcagcagcgg taaattattc atgattcgtg
    18001 ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag gatgagggta
    18061 ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc gtattgctgc
    18121 gatcccggga gacgggattg gcagagaagt ccttcctgaa gggattcgcg tgttacaggc
    18181 tgccgctgag cgctggggct tcgccttgag ttttgagcaa atggagtggg cgagctgcga
    18241 gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac ttagccgttt
    18301 cgacgccatc tattttggtg ccgtcggctg gccggacacc gttccggacc atatttcgtt
    18361 gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc tgcgcccggt
    18421 tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg acatcgattt
    18481 ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggca gagtgaatga
    18541 aggtacagag catgaagtcg tcattcagga atcggtattt acgcgtcgtg gtgtcgatcg
    18601 cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc taacttctgc
    18661 cactaaatcg aacggtttag ccatcagcat gccgtactgg gatgagcgag tggaagcaat
    18721 ggctgagaat tacccggaga tccgctggga caagcagcat attgacattc tctgcgcacg
    18781 ttttgtgatg cagccggaac gcttcgatgt ggtagtggcg tccaatttgt ttggcgatat
    18841 cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat ccgccaacct
    18901 gaatccggaa cgcactttcc cgtcgctctt cgagccagtc cacggttccg cgccggatat
    18961 ctacgggaaa aacattgcta accctatcgc cacaatttgg gccggtgcaa tgatgctcga
    19021 ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc tggccgcgat
    19081 tgaagaagtg attgctcacg ggccgaaaac accggatatg aaaggcaatg ccaccacgcc
    19141 acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca gtttatttga
    19201 accgcgtcac tgacgcggtt tttttattcg ttctttgcag taaataacct gcgtcatttc
    19261 accttttatt gtttccgttt cgtgttttat gg
//
LOCUS       DAYPHN010000089        19290 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000089 DAYPHN010000000
VERSION     DAYPHN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.089"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(44..946)
                     /gene="yihR"
                     /locus_tag="ACG0BW_002929"
     CDS             complement(44..946)
                     /gene="yihR"
                     /locus_tag="ACG0BW_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFK7259865.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQDYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDDYLFQLPAEQVYAVDEHANPTTLHHVDELNLNFTRATKIAATKIDH
                     TFKTANDLWEMTITHPQHLLSVSLCCDQPWVQVYSGEKLQRQGLAVEPMSCPPDAFNS
                     GTDLILLQPGKTHRLFFNIYGQRK"
     gene            complement(1014..2255)
                     /gene="yihS"
                     /locus_tag="ACG0BW_002930"
     CDS             complement(1014..2255)
                     /gene="yihS"
                     /locus_tag="ACG0BW_002930"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFK7259866.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNAVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(2271..3149)
                     /gene="yihT"
                     /locus_tag="ACG0BW_002931"
     CDS             complement(2271..3149)
                     /gene="yihT"
                     /locus_tag="ACG0BW_002931"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFK7259867.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3173..4069)
                     /gene="yihU"
                     /locus_tag="ACG0BW_002932"
     CDS             complement(3173..4069)
                     /gene="yihU"
                     /locus_tag="ACG0BW_002932"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFK7259868.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4237..5133
                     /gene="yihV"
                     /locus_tag="ACG0BW_002933"
     CDS             4237..5133
                     /gene="yihV"
                     /locus_tag="ACG0BW_002933"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFK7259869.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATDGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5167..5952
                     /gene="csqR"
                     /locus_tag="ACG0BW_002934"
     CDS             5167..5952
                     /gene="csqR"
                     /locus_tag="ACG0BW_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFK7259870.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6051..6650
                     /gene="yihX"
                     /locus_tag="ACG0BW_002935"
     CDS             6051..6650
                     /gene="yihX"
                     /locus_tag="ACG0BW_002935"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFK7259871.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPGYFAKVLC"
     gene            6644..7516
                     /gene="yihY"
                     /locus_tag="ACG0BW_002936"
     CDS             6644..7516
                     /gene="yihY"
                     /locus_tag="ACG0BW_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFK7259872.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7513..7950
                     /gene="dtd"
                     /locus_tag="ACG0BW_002937"
     CDS             7513..7950
                     /gene="dtd"
                     /locus_tag="ACG0BW_002937"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFK7259873.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            7995..8936
                     /gene="fabY"
                     /locus_tag="ACG0BW_002938"
                     /gene_synonym="yiiD"
     CDS             7995..8936
                     /gene="fabY"
                     /locus_tag="ACG0BW_002938"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFK7259874.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETLGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            9352..10239
                     /locus_tag="ACG0BW_002939"
                     /pseudo
     CDS             9352..10239
                     /locus_tag="ACG0BW_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10252..11166
                     /locus_tag="ACG0BW_002940"
     CDS             10252..11166
                     /locus_tag="ACG0BW_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7259875.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGSALMYATSSGPYYAQSLSGSPDIEMIKNRMAQLFTQLDASVRNKYRPAFDKWNELV
                     DKLNHERNTVPFACLTTILSTAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFNCDSLTAKELMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESSSYTVGMGV"
     gene            11862..12080
                     /gene="yiiE"
                     /locus_tag="ACG0BW_002941"
     CDS             11862..12080
                     /gene="yiiE"
                     /locus_tag="ACG0BW_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFK7259876.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            12299..12541
                     /locus_tag="ACG0BW_002942"
     CDS             12299..12541
                     /locus_tag="ACG0BW_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFK7259877.1"
                     /translation="MNSLAGIDMGRILLNLSNELFKQLDDLEVQRNLPRAELLREAVD
                     QYLVNQSQTARASAFGIWQGSEEDGVEYQRKLREEW"
     gene            complement(12723..13652)
                     /gene="fdhE"
                     /locus_tag="ACG0BW_002943"
     CDS             complement(12723..13652)
                     /gene="fdhE"
                     /locus_tag="ACG0BW_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFK7259878.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(13649..14284)
                     /gene="fdoI"
                     /locus_tag="ACG0BW_002944"
     CDS             complement(13649..14284)
                     /gene="fdoI"
                     /locus_tag="ACG0BW_002944"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFK7259879.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(14281..15183)
                     /gene="fdoH"
                     /locus_tag="ACG0BW_002945"
     CDS             complement(14281..15183)
                     /gene="fdoH"
                     /locus_tag="ACG0BW_002945"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFK7259880.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(15196..18246)
                     /gene="fdnG"
                     /locus_tag="ACG0BW_002946"
     CDS             complement(15196..18246)
                     /gene="fdnG"
                     /locus_tag="ACG0BW_002946"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(17659..17661),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFK7259881.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            18440..19273
                     /gene="fdhD"
                     /locus_tag="ACG0BW_002947"
     CDS             18440..19273
                     /gene="fdhD"
                     /locus_tag="ACG0BW_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFK7259882.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         4678 a         4742 c         5045 g         4825 t
ORIGIN      
        1 agtgaaatgt tcttcaatga ttttgactgc taatcaatac aagttatttt ctctgtccgt
       61 aaatattaaa aaacaggcgg tgtgtttttc cgggttgcag taatattaaa tcggtgccgg
      121 aattaaacgc atcaggcggg caactcatag gttcaactgc cagtccctga cgctgtaatt
      181 tttcaccgct ataaacctgc acccagggtt gatcgcaaca gaggctcacc gaaagcaaat
      241 gctgtggatg ggtaatggtc atttcccaca ggtcattagc ggttttaaag gtatggtcta
      301 tttttgtcgc ggctattttt gtcgcccgcg tgaaatttaa attcagctca tcaacgtggt
      361 gaagcgttgt cggatttgcg tgctcatcga cggcataaac ctgttctgcc ggaagctgga
      421 aaagatagtc atcaacagat gttaagttgc aggttaaata aggatgaata cccacaccat
      481 aaggtgcggt cacagtacca atattctggc ttgcgatctc aacggaaagg cccgtgcgag
      541 cgttgagcga ataaacaacc tgcgaagcca gcataaacgg atagccgtaa ctgggcggca
      601 gaaaagctgt cagggtaacg ctggtggcgg ttaattcgct aatttgccag tcacgccagg
      661 caagcagccc gtgaatagcg gcttttgaac tatgttcatt aatgggtaac tgatagtcct
      721 gcccctgata gcggtaacag ccgttggcga tccggttcgg ccacggaata agcactttac
      781 ccagatgcgc cagcggcatc tcttcgggtt tgtgcgggat gactaaatga caaccctgaa
      841 aggttaattc agccagacca gcaccaaccg tcacgatagt cgcgtgataa tcccccgcgg
      901 caagtgaaac ggtttgtcca ctgcaatgca tgttggtgat ttgcatacga ggcaccttta
      961 ttgaataacg acatacccgg catgtagcct gatatgccgt tatttcagct cagttatttc
     1021 gcgttaatgt ccagcaggcc tgcggcaaca gccggtgcca gaccaggggc taacgggata
     1081 cgcgggatca ccaggcaatg cagcaggtga taaatatcct gtttgccgtc ccagactttc
     1141 gtggtgactt tattgtccgc atccagctcc tgccaccagg aaccattttc atagtccatc
     1201 aggtacttaa tgcagtagtc ccaccatgtc tgataccagg tttcatactg gcgatcgccg
     1261 gtgacggtgt agagcgcgta agccgtaccc atcgcttcga cgataggcca acgcacacgt
     1321 tcgcgaacca ccggtttgcc ttcccagtca acggtataaa caatcccgtc cgcgccatcg
     1381 ggtgcccagg catcgcgcac ggtggcgtta aatagacctt tggcatcttc tagcagccag
     1441 gcaggcggct gttcacaacg ggcttccagg gctgcgtgga tgtgcagcat taaacggccc
     1501 cattcgatcc agtggcccgg cgtgccaccg aatgcgcgga agcggtgcgc cgggttatct
     1561 ttgttgtaat ccggcagcgg attccactgg gtatcgaagt gctcgttaac gcgataatga
     1621 ttatttctgg cgacgtcgtg gataatcacg gaagccacgc gaatcgcgcg atccagccat
     1681 tttttgtcgt gcgttacgtc ataaacaatc aggaaggctt ccaccgcgtg catgttggca
     1741 ttgccgccgc ggtactcttc ggttttgctg aaggcttcgt cccacgactc caggcacatc
     1801 tgctcttctt cactccagaa atatttttca ataatttcaa tggtgtaatc gagcagcttg
     1861 cgtgcttccg ggtgaccggt agtgacggca ctggcggcac ccagcagagc aaagaagtgt
     1921 tgatagcctt gtttggaggc atccaccacg ccctcgtcat tcacgcaggc ataccagccg
     1981 ccatattttt tatcgcgcag tgcgccattc atggctttga taccgtgatc aaccaacgag
     2041 taagcgccag ggcggcccat cgccgcagca acggaataaa cgtgcagcat acgggcggtg
     2101 atccacagat gcgtgcccat ctcttctttg atttgccctt tattgcccag ccagccaaag
     2161 ccagtcggca ctacggcatt cttaccaaaa tcaaagatgc gatcggtttc ctgttccagc
     2221 cagcggttgt gacttaaggt gttaaaccat ttcatttttg tatccccatc ttaacggcgt
     2281 ttagccatca tttcgtcgac gatatcgcca agttgttgta atttcggtgc gcaaacgtca
     2341 cgcagcatca gctcgttgtc tggtaaaccg acaaccgatg cccagacggc acgaccagcc
     2401 aggaagcccg atgcacccgc tgtcattgcc acgcgtacgg cacgcgggaa cagtttttcg
     2461 tcgacaccgg aagaaagtat cacccacggc atattgatat ggtcattcag acgttgtgaa
     2521 gcactgagaa gctcttgttg cggaccttta ccgtaaagcg gcatttcaac tttgtagagg
     2581 tcagcgccac tgtcgcccag ctctttggca gcatcgatga tcgcttgttc gcgatcgaat
     2641 ttatcgccac gacgtggtgg gcggacgact ggctcaataa tgcttaccag gccgtgtgag
     2701 tggcacagtt cgttaaactc tttcaccata tccagacgtt gctgcgcatc ttcatcacta
     2761 cgccacagca ccagcagttt taaggctttg ccaccgtctt gtttgatttg tagcggattg
     2821 attttgcggt caatgaccac gctatctacc ggaataccgt tgccaggaat gaactcatcg
     2881 gcggcgacaa tcatggcgca acttttggca atcgcgtttt gctcaaccac ctggcggtaa
     2941 cagaattgtt gatctaccag aatcgccgag gcataaggcg agagggtctt cgccgcgtta
     3001 actttgaaat cagttaaaac gctatcggct accggagcgg gtgcgccagc cgcagcaaac
     3061 atcatgcgca tggcttcgcg ctgatcgacc gccagcatgg caaaaccgcc cgatgcgcgc
     3121 gtaatgtcgt tgatggtgta cttattcatt catttattcc tttatccagt cgttacattt
     3181 ttactttggc agtcatcccg gcactgacac ggacctgttc cagaatggcg gaccagtcct
     3241 ggcgaccgcg acccgctgcg cgcgcctggc tataaacctc ccgtgaggcg gcccccagcg
     3301 gcattggcac atgcagctgg ttggcgacat caagggcgat accaagatcc ttatgggcaa
     3361 gatcgatcat gaaggcggga gaaagatccc cgctgaggac tttgtttggc caggaagtgg
     3421 tgaagtggcc tttaccggcg gcggtgccgc tcatcacctt gacggcaaca tcgaagggaa
     3481 gattcagggc ttcgcacaaa acagcggctt ctgccgaaag cgcattgagc gcgatgctca
     3541 tgtagttgtt gatgagctta acgcggatcc ccatgcccgg accgcccgcg ttgatcaact
     3601 cactgcccat cgccatcagg atcggtgtgg cgcgttcaac ttgttcagcg gtgccgccag
     3661 ccagcagtaa cagagtaccg gtaatggcat ttgcagaagt acggcctacc ggaacatcca
     3721 tcatgctgaa gcctttggct tgcatatcgg caatcaattt atcggtttgc agcgggtgga
     3781 tggtcgacat atcgatgacc agcgcatcgg tagataagct ttcgcaaacg ccgttttcac
     3841 cgaacaacac gctgcgcacc agatcgccat tcggcaacat ggtaatgata aattcggcat
     3901 ctttagcggc ctgcgccggg ttggcggcgg gagtcgcacc tttgtctacc agatgccgca
     3961 cagcttcggc gttcacatca aagacgcgaa gttgatgccc ttgctgcaat aaattgctcg
     4021 ccattggcga acccatttgt cctaaaccga taaacgcgat tgctgccata accctctcct
     4081 gaatacagtg atgtcacttt ttgtcattta tgacatgctt tgcttgtctg tttttgatcg
     4141 tatttgtaat ttatcgtcaa aaaattgaca gccgtcactt tttaaacaat tggtgaaatt
     4201 aaaatgaacg catcccaaaa tgcttaagga atgaccatga ttcgtgttgc ttgtgtaggt
     4261 attaccgtga tggatcgtat ttattacgtg gaaggattac cgacggagag cggtaaatac
     4321 gtggcgagaa attatacgga agttggtggc gggccagcgg cgacggcagc ggttgcggcg
     4381 gcaaggctgg gggcgcaggt tgattttatt ggtcgcgtag gtgatgacga cacgggcaac
     4441 agcctgctgg cagagctgga atcctggggc gttaacaccc gttacaccaa acggtataac
     4501 caggcgaaat cttcgcaatc cgccatcatg gtggatgcca aaggcgagcg gataatcatt
     4561 aactacccca gcccggatct gctgcctgac gcagagtggt tggaggaaat tgatttctct
     4621 cagtgggatg ttgtgctggc agatgtacgc tggcacgacg gtgctaaaaa agccttcact
     4681 ctggcccggc aggcgggcgt gatgactgtt ctggacgggg acattacgcc gcaggatatc
     4741 agcgagctgg tggcattaag cgatcacgcg gccttttcag aaccgggtct ggcgcgctta
     4801 acgggcgtga aagagatggc cagtgcgcta aaacaggcac aaacgctcac aaatggtcat
     4861 gtctatgtga cccaggggag tgccggctgt gactggctgg aaaatggcgg acgtcaacat
     4921 caaccggcct tcaaagttga tgtggtagat accaccggtg cgggtgatgt ttttcacggc
     4981 gcattggcgg tggcgctggc aacagatggg gatttagcgg agtcagtccg cttcgccagc
     5041 ggtgtagcgg cgttgaaatg cacgcgtccc ggtggacgag ccgggatccc tgactgtgat
     5101 caaacccgat cttttttgtc actttttgta taaaatgcca gggtgatggt ttttcgagga
     5161 attttcatga gccttaccga actaaccggt aacccgcggc acgaccaact cctcatgctg
     5221 atcgccgagc gtgggtatat gaatattgat gagctggcaa atctgctgga tgtctccacg
     5281 cagacggtcc gccgggatat tcgtaaatta agcgagcaag ggctgatcac gcgccatcac
     5341 ggtggcgcgg gtcgggcctc cagcgtcgtt aatacggcgt ttgagcagcg tgaggtttcg
     5401 caaactgaag agaaaaaagc gattgccgaa gcggtggcag actatattcc tgatggttca
     5461 acaatattta tcaccattgg tacgaccgtt gaacatgtcg cccgggcgtt acttaaccac
     5521 aatcatttgc ggataatcac caacagcctg cgtgtggcgc atattcttta ccacaacccg
     5581 cgctttgaag tgatggtgcc cggcggtacg ttgcgctctc ataatagcgg gatcattggc
     5641 ccttcagcgg cgtcctttgt ggctgatttt cgtgctgatt atctggtaac aagcgttggg
     5701 gcgattgaga gcgatggcgc gttgatggag tttgatgtaa acgaagctaa cgtggtgaaa
     5761 acgatgatgg cgcacgcgag aaatattctg ctggtcgccg atcacactaa gtatcatgct
     5821 tcggcggcgg ttgaaattgg taacgtggca caggtcactg cgctctttac cgacgagctg
     5881 ccgcccgctg cgctaaaatc acgcttacaa gacagccaaa ttgaaatcat ccttccccag
     5941 gaagacgcgt agaattttgt gaccgttgac cttgcttcat acctgaccac atagccaaac
     6001 ctatcctttc ccgctacagt taatttcttg tggcgcgaaa ggaggcaaaa atgctctata
     6061 tctttgattt aggtaatgtg attgtcgata tcgactttaa ccgtgtgctg ggtgcctgga
     6121 gcgatttaac gcgtgttcca ctggctacgc ttaagaagag tttccacatg ggcgaggcgt
     6181 ttcatcagca tgagcgtggg gaaattagcg acgaagcgtt cgcagaggcg ctgtgtcatg
     6241 agatggctct accgctaagc tatgagcagt tctctcacgg ctggcaggcg gtgtttgttg
     6301 cgctgcgccc ggaagtgatc gccatcatgc ataaactgcg tgagcagggg catcgcgtgg
     6361 tggtgctttc caataccaac cgcctgcata ccaccttctg gccggaagaa tacccggaaa
     6421 ttcgtgatgc tgctgaccat atctatctgt cgcaagatct ggggatgcgc aaacctgaag
     6481 cacgaattta ccagcatgtt ttgcaggcgg aaggtttttc acccagcgat acggtctttt
     6541 tcgacgataa cgccgataat atagaaggag ctaatcagct aggcattacc agcattctgg
     6601 tgaaagataa aaccaccatc ccgggctatt tcgcgaaggt gttatgctaa aaaccattca
     6661 ggacaaagcc aggcaccgta cccgtccact atgggcctgg ctaaaactac tctggcaacg
     6721 cattgatgag gacaacatga ctaccttggc aggtaacctt gcctatgtgt cgttgctctc
     6781 attagtgccg ctggttgccg ttgtttttgc gcttttcgcc gcttttccca tgttttccga
     6841 cgtcagcatt cagttgcgtc actttatttt tgccaacttt ctgcctgcta ctggcgatgt
     6901 tattcagcgg tatatcgaac aatttgttgc caattccaac aagatgaccg ccgttggggc
     6961 gtgcgggctg atcgtcaccg cgttattgtt gatgtactcc atcgatagcg cgttgaatac
     7021 catctggcgc agtaaacgag ctcgaccaaa aatttactcg ttcgccgtgt actggatgat
     7081 tttaacgctg gggccgctgc tggcaggggc cagtctggcg atcagttcct atttgctctc
     7141 cctgcgctgg gcgagcgatc tcaatactgt catcgacaac gtgctgcgta tttttccgct
     7201 gctgttgtcg tggatctcct tctggttgct gtacagcatt gttcctacca tccgcgtacc
     7261 taaccgcgac gcgattgtcg gcgcgtttgt cgctgcactc ctgttcgaag caggaaagaa
     7321 aggtttcgcg ctttatatca ccatgttccc gtcatatcag ctcatttacg gcgtgctggc
     7381 ggtgatcccc attctctttg tttgggtcta ctggacatgg tgtatcgtct tgcttggcgc
     7441 ggaaattact gtcactctcg gggaataccg caaattaaaa caagctgctg aacaagaaga
     7501 agacgacgaa ccatgattgc attaattcaa cgcgtaaccc gtgccagcgt caccgtggag
     7561 ggagaagtga cgggcgaaat tggcgcggga cttttggtgt tattgggtgt cgaaaaggat
     7621 gacgacgaac agaaagcaaa ccgtctgtgc gagcgtgtgc tcggctaccg catctttagc
     7681 gatgccgaag gcaagatgaa tctcaacgtg caacaggcgg gcggcagtgt actggtggtt
     7741 tcccagttta cccttgccgc agataccgaa cgggggatgc gtccaagttt ctccaaaggt
     7801 gcatcaccgg atcgcgcaga ggcgttatat gactatttcg tcgaacgctg ccgtcagcaa
     7861 gagatgaaca cgcaaacagg acgcttcgct gcggatatgc aggtatcgct ggtcaatgat
     7921 ggccccgtga cattctggtt gcaggtatga gccagcttcc agggttgtca cgggaaacaa
     7981 gagagagtat cgctatgtat caccttcggg ttccacaaac agaagaagaa ttagagcgtt
     8041 actatcagtt tcgctgggaa atgttgcgta agcccctgca tcaaccgaaa ggttcggaac
     8101 gcgacgcgtg ggatgcgatg gcgcatcacc agatggtcgt cgacgagcag ggtaatctgg
     8161 tggcggtagg ccgactgtat attaatgccg acaatgaagc gtccattcgc tttatggccg
     8221 ttcatcccga cgtgcaggac aaagggttag gaacgctgat ggcgatgacc ctggagtcgg
     8281 tggcgcgtca ggaaggcgtt aagcgcgtga cctgtagcgc ccgtgaagac gcggtggagt
     8341 ttttcgccaa gctggggttt gtcaatcaag gggagatcac cacgccaacc accacgccga
     8401 ttcgccattt tttgatgata aaacctgtcg ccactctgga tgacattctg catcgcggcg
     8461 actggtgcgc gcagctgcaa caggcgtggt acgaacatat cccgctaagt gaaaaaatgg
     8521 gcgtgcgcat tcagcaatat accgggcaaa aatttatcac caccatgccg gaaaccggca
     8581 atcagaatcc gcaccacacg ttgtttgccg ggagtttatt ctcactggcg acgctcaccg
     8641 gttgggggct tatctggctg atgctgcgtg aacggcacct cggcggaacg attattcttg
     8701 cggatgcgca tatccgctac agcaagccga ttagcggtaa acctcatgcg gtagccgacc
     8761 tcggtgcctt aagcggcgat ctcgaccgtc tggcgcgcgg gcgcaaagcg cgggtacaaa
     8821 tgcaggtcga aatctttggc gacgagacgc tgggtgcagt gtttgaaggc acgtatatcg
     8881 ttctgcccgc gaagccattt ggcccgtatg aagagggcgg gaacgaagaa gagtagctga
     8941 tggagagggg cggtgctgcc tctctcattc tttcaggtca tttacttcag tctctgacta
     9001 ttccggttat attgaataag aactctataa atttttattc ttcgttggca ccatacgtgt
     9061 cttgctacat cactgttcag gagcatttaa atattatttg tagagctgag tggcaattat
     9121 cgctgataat gcttaattca tcatcttagt tcatgtttga gcgatttttg atagtcaaaa
     9181 ccccctctgg tgccatactt ttctcataaa tatttttaaa atctatagct aacaaacatt
     9241 atgcaaagat aatggattgg cgtcattgtc agtatcattc atttactgct tctggatgtt
     9301 gtaaataatt atgtggcatg tttgctctta aaccgtggga aggaggctgt aatggttgcc
     9361 aatattaatc tcattaaaca agaaagttat tctattgtta atttagaaaa acaactaatg
     9421 agtaatgaat caccgaagca aacgctatta aatgattttt cacatcgcct taaaatggaa
     9481 ggtgaggagc aactgtgcga ttgtgttaac gaacttaaac tcgggttgtc gagtaataat
     9541 aaatatgacc tgtttaaaaa taaagaaacc tcactgtgga aacgcaaagt accagaaagt
     9601 gcagcgctgg caatgacggg aacagtatat aaattatttg gtagtcgctt actcaacgcc
     9661 cgcaatcagt tagataaaaa tctcctcgaa atggctatgc aaatggttta tggcaaagat
     9721 atggtgacaa agtccgggga tatattgatt gatatacaat taaataagga tggcagcctc
     9781 cagagtaagc actacacatt tgatgtaggt gaagttataa acagttataa tattgacctc
     9841 tttgtgaatg cttcgagtat taatcaaact tacttacatg ataaaaatcc tggtgacgtt
     9901 attagcctgt atggcacttt agataacatt cctcttgtag ttaaggaatg cacgggtaaa
     9961 aatcatcaaa ttaacgttaa agccaaattg ccacaagaac gaagtgaaaa agttgaggat
    10021 atgtgtgaaa gaatgagagg tggcatctct atgtttaacc acactacaaa aacagctggt
    10081 aatatagagc ataatttgca gctaagtttt ctcgtggatt cacgaccgtc gatcacaaat
    10141 aaatactcag ccaaagatgt aggggaagat atacttgcat tgcccgtcaa cgtatatcat
    10201 gcagatttta aaattaaaat caataacgca gtagagaagg cttcgtcaat tgtgttatgt
    10261 gctgtgcaaa aaaatctcca tgcagcaagg aaggttctta gcgggataat aggaaatgtg
    10321 ccaaaaaata ataaaccaaa aacaatcatt tttgatttac gttttcttag tgcaattaag
    10381 cagaaagttt ttttgggcag tgaaaaaccg ttgtttgtaa agcataccgc aatgatgagg
    10441 gaggcatgta aggaattacc gcagtcagtg gaatatgttc ctttagcctg tgaagcgcat
    10501 agccatcgca gcgtcgccct tgccaaaacc gtagctgcgc ccatcatggc gacaggcgtg
    10561 ggatcggctt taatgtatgc aacatcatca ggaccgtatt atgcgcaaag tctttctggt
    10621 tctccggata tcgagatgat aaaaaatcgc atggctcaat tgtttaccca gttggatgct
    10681 tctgtgcgca acaaatatcg cccggctttt gataaatgga atgagctggt agataaatta
    10741 aatcacgaaa gaaatacagt tccgtttgcg tgtttgacca ccattttatc gacggcgata
    10801 aatgaaactc ctgaggggga tacgaatgtt gccgttgtga tggggtgtaa atctgcgaaa
    10861 gatcgcacga tatccattgt ccttggcaac tcgatgttgc aaacactctt tgaaaaacgc
    10921 ctcgccgatg atggagaaat agaaaatctt tttgatcaac agggatattt taactgcgat
    10981 tcattaacag ccaaagaatt aatgatgtta aaagaccttt ttgatattcg agttcttcat
    11041 ctctctaaca aatttaatgt tgggttgcaa ggtaatatca acacggatgt tttacaagat
    11101 tcgtttttta aaaatgtcga tttcattcat gaatctagct cgtataccgt agggatggga
    11161 gtctgacggg taaaactcat taggattatc agaattcgga caatgcattg caaaaatgac
    11221 attcaaagag agatgtgtgc aggcgcttag ccgcgagggg catagcgaag ccatacagaa
    11281 agcactagca ttcagaactc ctgtgaaagc ggcatagtgg ccgcctgtgc aggagttttg
    11341 ctatgacccg tatcgtgctg accgttgcca ccctatgatg cgattaaaaa aactcatcgt
    11401 cgcgccgatg attgacgaca atgctagcgg atgatgtcag gggtgttccg gaaggaattc
    11461 cttgtttccc gaactgttac ccacaaaatt ataacgacga gcaagtaaag accggttggt
    11521 ggcagcattg aacggtctgt gatggatgtt aaatttcacc ttaaatcctg gtgctgttag
    11581 gatgctgacg tttttcgggt acaccattca gattatgcaa ataaagatcc aatcaaaaaa
    11641 taaatatccc tgaatataat atattcatag cacccataac tcatcttggg cgataactaa
    11701 accatttttg agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct
    11761 cgcaaaaaca aaacaacatg caaaaatccg ctttttttaa atgattttcc ggcgtaattt
    11821 tcgcacatac attaatgagt aatatatgta aggtggtggt aatggcgatg aatacggttt
    11881 ttcttcattt atcagaagag gcaattaaac ggctgaacaa gcttcggggc tggcgtaagg
    11941 tttcacgatc tgcaatttta cgcgaagcgg tagagcaata tctggagcgt cagcaattcc
    12001 cggtgcgtaa agcaaagggt ggcaggcaaa agggggaggt agttggtgtt gatgatcagt
    12061 gtaaggagca taaggaatga caaaaatttt tcattcttga atataaaaaa acagatgccc
    12121 ttattctggt attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca
    12181 tcatatctgg aaaacgcctc gcaaaaataa aaaatgatgc gtaaataagc tttctttaaa
    12241 gattcggcaa gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat
    12301 gaactctctg gcaggtatag atatgggcag aattttactc aatttatcga atgagttgtt
    12361 taagcaactt gatgatctcg aggtgcagcg taatcttcct cgtgcggagc tattacggga
    12421 agcggtagat caatacctgg taaatcaatc gcaaacagca agagccagtg cttttggcat
    12481 ctggcaaggg agtgaggaag atggtgtcga ataccagcgt aagctgcgcg aggaatggta
    12541 atggcaaaaa gatttggcta tttgatatcg atatccttat cgattttctc agtgtgaaaa
    12601 ttgaggcaaa acaggcactg gcggcatagc ttgaggttgt aacgcggaat acgagagata
    12661 gtgtcgtcac accctatgag ctgtaatccg ggcgaccagc gccacccgga aaacgtcaga
    12721 gattactccc cttcacccgg gaacaggaac gggttgatgg aactgcgggc gtagccttct
    12781 tgctccattc gcgcgtccag taccagagag gcgaggtcat ctgccacggc ttcaactttc
    12841 ggttcttttt cctgataaag aatcttcagg taagtgccac agtcatcgca gctttcggct
    12901 ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca gtttgccgct ctgttcacag
    12961 ttgctgcatt ttacgcgcac cacgtgccat tcggtttcgc acaggttgca gtgcaggtaa
    13021 cgcagaccct gagtggtgcc aatttgcacc atgctggaca ccggcataga gccacaaacc
    13081 gggcaatatt gacgttgttc gccatattca gcgcgggctt tgccggggat cagattagcc
    13141 atctgcgccc agtagagcga cagtgcagcc cagataaacg gcgctttatc gctgctgacg
    13201 gacgagaaat cagaggcaaa cagtgcgctg gccatatctt ccagctcctg agtcgatgcc
    13261 ttctccagat tctcaatcac tgccagcgcc gggccgctca tttcaggttt cagctcagca
    13321 atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg aatatccagc
    13381 gggggcttgc cttgtgcgct ggcttcttta atgcgcgtgg tcagatccat ctccagcgga
    13441 tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg caggtaatca
    13501 cccagcggat tattttctgc cagctcgcgc agacgctcgg cgcggcggtt gtataaattc
    13561 ttgagccgag ggaacaataa cggcggaatc atatccgccg tacgtttctc gctcgaaccc
    13621 agctcatctt gcgggattat gcgaatactc attcagcttt cttttccgtt gtcttgcgga
    13681 cctcacggta ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc catccttcca
    13741 ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc atgataacca
    13801 caattaacgc cactgcggca aatgaatgca gcattaacgc gaatcggatc accgggattg
    13861 agaaagcagg cgcaaaataa ggacgccaga taatcacgcc gctcaccagc agcagaacca
    13921 ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa gttataacgc ccggtgtcac
    13981 ctacttcctc gttgacgacg atcttacgaa tattcttcgc ccaaaagata tcatcccgat
    14041 tgattaggtt gtgatgccag taacggaaaa acatgatgat gaacgaggca aacataacca
    14101 cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt gcccatgatt tgcatcaacc
    14161 agttgaagga cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg aagcagaagg
    14221 cggtgatcca gtggttgata cgttccggcg cggtgtagcg cacgatggtg tcacgtcgtt
    14281 tcatttgcgc tcctcgtctt tctcttcgtg cagattattc tcttcctcat ccgcacggtt
    14341 cggaccgaca cccacgtagt ggaagatact ggctgcgaag gtagccgcaa agccaacagc
    14401 tgcgagcggt ttccagatgc ctttccagaa tttcacggtt tcgctgattt ccgggttctc
    14461 cggcaagcca tgatacagat ttggcttgtc agcatggtgc agcacgtaca taacgtgtgt
    14521 accaccaacg cctgccggat cgtacagacc cgcattgtcg taaccgcgag ttttcagctc
    14581 cgccacgcgc tcgctcgcca gcgttttcat cgactctttc gtaccgaagt gaatcgcgcc
    14641 cgttgggcag gtcttcacgc aggccggttc ttgcccaacc accacgcggt caacgcacag
    14701 cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga acggacagcc
    14761 cgcaatgcaa taaccgcagc cgatgcactg ctcggactgg aagtcgacga taccgttggc
    14821 atactgaata attgctcctt ccgccgggca cgctttcagg cagcctggat cggaacagtg
    14881 catacagccg tctttgcgga tcagccattc cagtttgtcg ttctgttcca cttccgagaa
    14941 gcgcatcacc gtccacgatt tggcacttaa atcattgggg ttgtcgtaca ccccaatgtt
    15001 attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac aggctttaca
    15061 gccgatacag gtggtgacgt cgatgagttt cgccacttct tcctggaagt cccgcgcctg
    15121 aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg atatcttgag attgataagc
    15181 catatgtcgt ctccgttaca ccttttccac attcacaagg aaggacttaa actctggcgt
    15241 ctgcgtgttc gcatcaccaa cgaatggtgt taacgtattg gcaataaagc cttttttcgc
    15301 aacaccttca tagccccagt gaatcggaat accgatggta tcgatatctt tgccgttcgc
    15361 tttcagcgtg cgaatacgtt tggtcaccac cgctttggct ttgatatagc cacggttgga
    15421 ggagactttc acggtatcgc cctgggcaat gccaagttta ttcgccagcg actcaccgat
    15481 ttccacaaac tgctctggtt gcaaaatcgc gttcaacagc gcgtgtttgg tccagtagtg
    15541 gaagtgctcg gtcagacgat aggtggttcc gacatacggg aacttatcgg ctttacccaa
    15601 tgcttcggcg tcgtctttaa agatacgtgc agccgggttc gagataacgt ttggatgcag
    15661 cgggttagtc cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg gaccttccgc
    15721 catcttatcg agggcaaaca gacgccccat gccttcctgc tgcatgataa acggcccgac
    15781 gccactgccc ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc acttggtgcc
    15841 gtcccatttc agcaactgac gcttcggatc ccacgggtta ccctgcggat ctgcggaggc
    15901 gcggttatac agaatacggc ggttaagcgg ccatgcccat gcccagccca gcgtgttacc
    15961 gaggccagac ggatcggcgt tatcacggcg tgccatctgg ttgccctctg gcgtccagct
    16021 accggcgaaa atccagcagc cacaggaggt tgtaccgtca tcgcgcagtt gggcgaacga
    16081 gctaagttgt tggcctttct tgacgataac cgcaccggtt gccggatcgg taatatcggc
    16141 cagcgcctta ccgttactct ccatcgccac ttcttctgaa gatggctcat gcggaatggc
    16201 gtagttccag gtcatgttca gcacctggtc cgggttcgcg ccaccctgtt cggcatacat
    16261 cttgcgcaag cgcaggaaga taccggagag gatctcgccg tcagtcagcg caatccccgg
    16321 ggcgtccgca cctttccagt gccactgcaa ccagcgacca gagttgacga ttgaaccgtt
    16381 ctcttccgcg aagcaggtcg atggcagacg gaacacttcg gtctggatct tcgacgagtc
    16441 aacttcgttc agttcaccgt ggttctgcca gaagttagag gtttcagtgt tcagcgggtc
    16501 gatggtcacg aggaacttca gtttcgacag acagccgatc actttgtttt tgttcgggaa
    16561 tgaggcaacc gggttaaagc cctggcagat atagccattg actttgccct ctttcatcat
    16621 ctcgaagtat tgcaggacgt cgtagccttt atcccacttc ggcaaccagt caaagcccca
    16681 gctgttttct gccgtcgctt tatcaccaaa gaaggccttc atcatggaga cgaagaattt
    16741 cgggtaattg ccccagtagt taacctggcc ttccagcagc ggtttcggcg tgttggcggc
    16801 aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac ctggcaggct
    16861 ctgcgacagc agccccaggt cggtcagccc ctgaatattg gagtgaccgc gcagggcgtt
    16921 aacgccgccg ccagccatcc ccatattgcc gagcagcagc tggatcatcg ccatcgtacg
    16981 aatgttttgc gcaccaatgg agtgttgcgt ccagccgagg gcatagagga acgacgctgt
    17041 tttatcgtga gcactggttt cggcgatgta ttcgcagact ttcaggaacg cgtcttttgg
    17101 cgtaccgcag atgttttcaa ccacatctgg cgtataacgg gaaacgtgct gtttcagcaa
    17161 gttccacacg caacgcggat gttgcagcgt ggtatcgcgt ttggcgaagc cgttttcgtc
    17221 cagttcgtaa gtccaggagg atttatcgta cttgcgtttt tccgcgtcgt agccggtgaa
    17281 caggccatct tcaaagccgt aatcctcacg cacgatcagg ctggcgttgg tataggcttc
    17341 ggtgtattcg cggttgaatt tttcattgtt cagcaggtac agcaatacgc ctgacaggaa
    17401 agcaatgtca gtcccggaac gaataggggc atagtagtca gccaccgccg ccgtacgcgt
    17461 aaagcgagga tcgatcacaa tcagcttcgc gccgttgtga attttggctt ccatcgccca
    17521 gcggaacccg accgggtgag cttcagcggc attaccgccc atcaccacga cgaggttggc
    17581 gttcttgatg tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg gagcaagact
    17641 tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca tccccagcgc
    17701 gcgggagaat ttttgcgtta aatagccggt ttcgttactc gacgcggaag cacacagcat
    17761 cccggtggag agccagcggt taacagtcac gccttcggcg ttttgcgcaa tgtagttagc
    17821 atcgcggtct tctttcatca gtttggcgat gcgatcaaac gcctcttccc aactgatttg
    17881 ctgccattta tcagaacctg gcgcacggta ttccggaaac ttcagacggc tttcggagtg
    17941 gatgaaatcc accaggccag cgcctttcgg acaaagtgca ccgcggttga ccgggtgatc
    18001 cggatcgcct tcgatatgga agatagatgc tttggcgttt tttgctccgt caccgaggct
    18061 gtacatcaac agcccacagc ctacggaaca ataggtgcag gtattacggg tttcgcgggt
    18121 gcgcagcagt ttatactgcc gggtttccgc gagtgctacg ctgggtgcaa aacccagtgc
    18181 cgctgccgtg gtgcctgcca taccgccagc gcagatctta aagaactgcc ttctgctgac
    18241 ctgcatggat tgctccttgt ttcgacattg tcacgtccca tttacattcg cttgctgcgt
    18301 gtgcagggag tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc agaattggct
    18361 caatttccct ccatccagga agggtttgta acagaatacc ataatgttgg tgtgtgtgtt
    18421 cttatctggt taagagaaag tgaaaaaaac acagcaaaaa gaaatcgaaa atgtgacaaa
    18481 tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat gatttacaac acccacggtt
    18541 agatgaggtc gcggaagaag ttcccgttgc gctggtctac aacggcattt cgcatgtggt
    18601 gatgatggcg tcgcccaaag accttgagta ctttgcgctc ggtttttcgc tttccgaggg
    18661 gattatcgaa agtccgcgcg atatcttcgg tatggatgtc gttccttcct gtaatggtct
    18721 ggaagtgcaa attgagcttt ccagccgccg ctttatgggg ttgaaggagc gccgccgggc
    18781 gctggcggga cgtacgggct gtggcgtatg cggtgtagaa cagcttaacg atatcggtaa
    18841 accggtgcag ccgctgccgt tcacccagac gtttgatctc aacaaactgg atgatgcatt
    18901 acgtcatctc aatgatttcc agccagtggg gcaactgact ggttgtacac acgccgctgc
    18961 ctggatgttg ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc gccatgtggc
    19021 gctggacaaa ctgttaggcc gccggtcaca agaaggggaa agctggcagc aaggtgcggt
    19081 gctggtttcc agtcgtgcca gttatgaaat ggtgcaaaag tcggcgatgt gtggcgtaga
    19141 gattttgttt gcggtgtctg ccgcgaccac acttgctgta gaagtggctg aacgctgtaa
    19201 tttgacgctg gtgggctttt gtaaaccggg tcgggcaacg gtttataccc atccgcagcg
    19261 tttgagcaat taattaaaat aagtcctgcg
//
LOCUS       DAYPHN010000090        18721 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000090 DAYPHN010000000
VERSION     DAYPHN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.090"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..581)
                     /locus_tag="ACG0BW_002948"
     CDS             complement(<1..581)
                     /locus_tag="ACG0BW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7259883.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVA"
     gene            complement(578..1354)
                     /locus_tag="ACG0BW_002949"
     CDS             complement(578..1354)
                     /locus_tag="ACG0BW_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7259884.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVTVYILLMLIVWMAGEYFEITNFLQLVATWSLLIILSCGVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFISLALYRTREEAMLTS"
     gene            complement(2411..2947)
                     /gene="yghD"
                     /locus_tag="ACG0BW_002950"
     CDS             complement(2411..2947)
                     /gene="yghD"
                     /locus_tag="ACG0BW_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFK7259885.1"
                     /translation="MLRDKFIQYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPNIIAARKSGTTTPVQL
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLNWLNALDEKYALRVTQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            complement(2949..4127)
                     /gene="gspL"
                     /locus_tag="ACG0BW_002951"
     CDS             complement(2949..4127)
                     /gene="gspL"
                     /locus_tag="ACG0BW_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFK7259886.1"
                     /translation="MSSILEIFFPLCATDPIRWQRRTADVECGIWPDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEELSAWADADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(4124..5101)
                     /gene="gspK"
                     /locus_tag="ACG0BW_002952"
     CDS             complement(4124..5101)
                     /gene="gspK"
                     /locus_tag="ACG0BW_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFK7259887.1"
                     /translation="MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVEQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5098..5703)
                     /gene="gspJ"
                     /locus_tag="ACG0BW_002953"
     CDS             complement(5098..5703)
                     /gene="gspJ"
                     /locus_tag="ACG0BW_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFK7259888.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGVRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(5700..6071)
                     /gene="gspI"
                     /locus_tag="ACG0BW_002954"
     CDS             complement(5700..6071)
                     /gene="gspI"
                     /locus_tag="ACG0BW_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFK7259889.1"
                     /translation="MKRGFTLMEVMLALAIFALAATAVLQTASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLNTGNALLQAVDIEVSLH
                     EDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(6068..6631)
                     /gene="gspH"
                     /locus_tag="ACG0BW_002955"
     CDS             complement(6068..6631)
                     /gene="gspH"
                     /locus_tag="ACG0BW_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFK7259890.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYTLELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(6635..7090)
                     /gene="gspG"
                     /locus_tag="ACG0BW_002956"
     CDS             complement(6635..7090)
                     /gene="gspG"
                     /locus_tag="ACG0BW_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFK7259891.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7107..8330)
                     /gene="gspF"
                     /locus_tag="ACG0BW_002957"
     CDS             complement(7107..8330)
                     /gene="gspF"
                     /locus_tag="ACG0BW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFK7259892.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLAGYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVRLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(8330..9823)
                     /gene="gspE"
                     /locus_tag="ACG0BW_002958"
     CDS             complement(8330..9823)
                     /gene="gspE"
                     /locus_tag="ACG0BW_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFK7259893.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(9823..11883)
                     /gene="gspD"
                     /locus_tag="ACG0BW_002959"
     CDS             complement(9823..11883)
                     /gene="gspD"
                     /locus_tag="ACG0BW_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFK7259894.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDANGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(11913..12872)
                     /gene="gspC"
                     /locus_tag="ACG0BW_002960"
     CDS             complement(11913..12872)
                     /gene="gspC"
                     /locus_tag="ACG0BW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFK7259895.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPLRADAKPFDKNDMQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEKRPTIAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(12890..13300)
                     /gene="gspS2"
                     /locus_tag="ACG0BW_002961"
     CDS             complement(12890..13300)
                     /gene="gspS2"
                     /locus_tag="ACG0BW_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFK7259896.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(13366..14175)
                     /gene="pppA"
                     /locus_tag="ACG0BW_002962"
     CDS             complement(13366..14175)
                     /gene="pppA"
                     /locus_tag="ACG0BW_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFK7259897.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(14331..>18721)
                     /gene="sslE"
                     /locus_tag="ACG0BW_002963"
     CDS             complement(14331..>18721)
                     /gene="sslE"
                     /locus_tag="ACG0BW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFK7259898.1"
                     /translation="PEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRIT
                     GATCNGESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQE
                     LAASDDKKSNAVSLVTSSNSCPADTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKL
                     VNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSE
                     GRLVDSMGNGVVGVNYYTSSGRGVTGENGKFNFSWGETISFGIDTFELGSVRGNKSTI
                     ALTELGDEVRGANIDQLIHRYSQAGKNDEREVPDVVRKVFAEYPNVINEIINLSLSNG
                     EALSEGDQTFERTNEFLEQFESGQAKEIDTAICDSLGGYNSQRWFSLTARNVNEGQIQ
                     GVINKLWGVDKDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKN
                     YWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGN
                     PHYNSILRCPNGYSWEGGVDKNGQCTRNSDSNDMKHFMQNVLRYLSDDKWTPDAKASM
                     TVGTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFE
                     YVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASG
                     FVRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSK
                     TGEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKI
                     FEKFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAA
                     DLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNKIEYRYENDKDDEL
                     GFKTFTEFLNCYANDAYTGGTQCSDELKKSLVDNNMIYGEKSVNKAGMMNPSYPLNYM
                     EKPLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTG
                     LWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPKVTKTYELKANGEVK
                     FTVPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGAWKNALNSPAPLGELESDAFV
                     YTTPKKNLEASNYKGGQEQFAEELDTFASSMNDFYGRDGENGKHRMFTYKNLTGHKHR
                     FTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGA
                     TEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLK
                     EWAEKNFDIKQWYPEGSLPEFYSEREGMKGWNLFQLMHRKARGDEVGKTKFGERNYCA
                     ESNGNTADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTL
                     ASLKLPKPEQGPETINKVTEYSMPAE"
BASE COUNT         4282 a         5235 c         4559 g         4645 t
ORIGIN      
        1 atcgccacat cacaaaactc tttcaatgaa ttcaaactat gtgaaaccat caaaaagcta
       61 gactctttat gcctttcttt aaacaattga gcgcacttct ctttaaacct ggcatcaccg
      121 accgctgtta cttcatcgac gatataataa tcaaacttaa atgccatgct taaaccaaag
      181 cctaaacgcg atcgcatacc tgaggagtaa gttttgatcg gcatatcaaa gtacttaccg
      241 agttcggcaa attcttcgac aaactcaatt ttctctttca gctcttcttg cttcgcgtat
      301 aaccgcgcaa caaattttac gttttcgcgt ccggttaaac ttccctggaa tccaccggcg
      361 aggccgactg gccatgatat cgttttattg gtgatgatct ttccgctatc ggggcggtca
      421 atgccaccaa tcattctcag taacgttgat ttacccgcac cattacgacc aataaaggca
      481 acgctttttc ctgaagggat ttcgatgttt aaatccttaa acacatagtg tcgccccgtt
      541 ggcgtacgat atgacttcgt caaattctca atcttaatca tgatgtcagc attgcctctt
      601 cacgcgttcg gtataacgcc agactgatga acagggtgac cagagtaaac attgccaggt
      661 agttcagact cacgccttcg ctgatatagc caggcataac tgcctcgcgg cttaactcta
      721 cgacatgcac taatggattc cagagtagat atgaccagta ttgcttcgga atcgagtgta
      781 gagggaacat gatgcaggag atgaaataca gcggcttaag cagtatcggc agaacctttt
      841 gcatttcggg gaaagtttta ccaacgacca tgaatattaa accgacgcca catgaaagaa
      901 tgatcagcag gctccaggta gccacaagtt gtaaaaaatt tgttatctcg aaatattcgc
      961 ctgccatcca gacgataagc ataagcaata tataaacagt aacgtaaatc agtgtctcaa
     1021 gcaatgcacg cgcaatgatc gtatcgatgg gttttactgg tcgataatta aacagcccct
     1081 gattcgcttc aatagcacct acagaacgat tgctgatact gctaaagata aaaaagggaa
     1141 tcaggccatt aagtaaaaac accgggaatg agatgtctgg catcgtgcgg tgcataatgt
     1201 aaccaaaaat gcccaacagt atcagcaaat gcgcagaggg ttcgagaatc gcccacaaat
     1261 atcccagacg gaacttacca aagcgtgttc gtatttctcg tagaaataat gcctctacgg
     1321 tgactttctg gacttcaaat ccacttcttg ccatttgatg atgtgatcct aatctcttca
     1381 ggtacgctac cgcccttggc ttaacagcta ccaatacact ggaggtaatg cttatttgta
     1441 actggataaa atttactcag acggtttgac ctttttaata gccgcaaaaa atttcagcag
     1501 aaattatttc cgcagagata aatagatcta tttccgtctc gtttcttcta atccattctt
     1561 caggcatgaa tgtattcggt cttctggtaa tttcagaaaa ctggccgttt tagaatgcag
     1621 agttaatttc tcaggaaatt atttttcact attactgtta actacttgat tataataccc
     1681 cctatggatt ttatgcgtaa caatacctgc aatctgtttc aaaccaggac gcatgcgatt
     1741 gattaccaat aaattgttta gcaagaaact atttccctat ttaaaattca ctcatgtact
     1801 tcttatttat atctacagcc ccctctttac agtcacattt gtgatttaca tcacatttaa
     1861 acggtattat ttaacaccta ttaataattc cgtgagtaat tattttcgtc aattaaaata
     1921 tacatcttga ttattttgtg catttatttt aaccagcgtt acagccagca ttatccctca
     1981 gtagatatac ctgagcagac atttcttaaa atcgacgaat agtttccctg gaaattatta
     2041 tgcttatttg aaattccctt tctgattatc tccaggaatt ttactaattt ccagactaca
     2101 caaaatacag atcacataag aattatccta tatgttttga cctatcaatt gttactcaat
     2161 gatttattac aactattaat aatcataatg tgtcaaaaca cttaagcatc tttacagtct
     2221 tagaaaggtg tcgattaacg aaggctacat ggtgaaaaac aggttccttc agtgttaccg
     2281 aatattccgt ggtggtctgg agggagataa ttcttgctac atcgaaactc agcccggtga
     2341 gcgggtttga cagttccgca ctcaccattg ggctaagggt tatcaggtgg ggttaaggaa
     2401 atggcaatac ctacccccgt ccaaactcca gtcgctgcac attcaccatc ccaggcttct
     2461 cacctgcgct gacatcaatt tgtgtcaccc gcagcgcata tttttcatcc agcgcgttta
     2521 accagttcag caggtcatta aacaccacag gttctatcca gacctgaata ttctccccgc
     2581 gctcggctat ccgcttgatg accacagagt gcgccgacgc actgtcactg ataacccgcg
     2641 atagctgaac gggcgttgtc gtgccggatt ttcgcgccgc aataatattc ggcgcagtgc
     2701 tcttcagtcg cgcgttcatc gccacgagct gctgcaacat tgtctcctgc tgctcaatcc
     2761 gttcgctcaa cggctgccag atgaggacgt aatatccgac gctaaacagg aacaccaccg
     2821 ctgccagtaa catgcctttt tcacgcggcg aacgccccgc caggtgctgt gctagccagt
     2881 gttcgccacg gcttaactgg cgttcacgcc attgctgaaa atattgaata aatttatcgc
     2941 gtaacatgtt atttcctccg caacgttacg ccgccggaaa ccgcatcacc ctctttctgt
     3001 aacgcgtcct gctgcacaac ataatctgcc gccagtgcgc tacgcagttt gtcgaagctg
     3061 gcaaagttcg cagcccgtag ctggaggtga agcgtctggc gtttttgatc aaaggtgaaa
     3121 ccacgcattt cgatgtcggt aagtgacgct gatttcaggg tactggcgat cgctgacaat
     3181 tctgcgagca gccgggtatc gtcggcctgt gggcgatatt ttttcagcgc catcgtcacc
     3241 tgagagcgta aattcacaat ccgcttctgc tccgggaata gcgttaagaa ctgtttctcc
     3301 gcctgggtgc ggctttgcgc cacctgttcg ctgacgctcc ataacgtcac gccccgttcc
     3361 actaccagcg caaccagaat cagcaatatc ggcagaatca tcacccgcca gcgcgcccac
     3421 tgttttcggt agctgacacg aggctgccac ggccctgtta gcaggttccc ttccggttcg
     3481 ccataagtgg taacggctgg cagggccgta actgtcaggc gttcagcgtc tgcgtcagcc
     3541 catgcagata gctcttccgg cgcgatcccg actacggtta gcgaaagcgg taaattctgc
     3601 tcattgagct gtgcgcggaa catgaccgga gccagcgccc gcccggcgct ccatccccgg
     3661 cattcatcga tgcggcagat aacccgttgc gcatcgccag ccatatagcc acaaggaatg
     3721 gacatccagt ctggcgcgac ggtagcgcgg gtgatgccgt ttgcctgcaa ccactgcgca
     3781 atgttgcgca tatgctgctg gtgaatcaca gctacggttg ccagttgctg gtcgattttc
     3841 aacggggcga aatgcagttc atcgatatcc tggttcagtt cttcttccag taaggcgggc
     3901 agaatcgtcg gtatctgctt gcggggcaca tcaggcagtt caacctgcca gacgctgatc
     3961 cattcgccgg gaatgtagag tcgaatcgca tcagtttgca gccattgctg aagctgttcg
     4021 ttagcgacgt caggccagat gccgcactcc acgtcggcgg tacgacgctg ccaacggatg
     4081 ggatcagtgg cgcaaagcgg gaaaaaaatc tcaaggatag aactcactca ctttctcctg
     4141 tctgatgcca gagaacagaa aagtgttgtg ggcccatgcg gacaattaac gaattcatcg
     4201 tcagttcaat ctcattcacg gtgatatctg aacgcagcca gaagtaattg ctgtccacgc
     4261 tcaggacggt ttttagctgt tttttagttc gctcatcgac gtcagcaagt agcggctgtg
     4321 caagaaactg ctcgacatct tcccagccct tcgccggacg ttgttgtaat aatgcccgcg
     4381 cctgaacagg gcttaaccac gggtcaaaca gcgcctcaag aatcacactt tgcgtgacat
     4441 ctaatgtatt gatgttgatt tgctggcggg ccatcggcag cgcacaaacc aacggtttca
     4501 gtttttgata aagcccggcg tccattccct gtaccacgcg catttcactg atatccgcca
     4561 gcggttgatt cgcggcgtag aacggcaccg aacgggcaag atactcgctg tcttcacgac
     4621 ccagacgcgt ctgcacgctg cggtcttcgt caataaactc ccacaggctt tcggctatca
     4681 gttcggcccg ataagcaggc acatcgaggc gcgagatcag ggcaatcagt tgttgtaccg
     4741 cgagcggacg cgacgccgtc gtcggctgag cgagggcatt caggttaaag caggcctgtg
     4801 cgtcacgcag agtgacggcg atttgcccct gcggcagtgg gaaaaaacgc gggccagaag
     4861 cccacggctg tgccagatgc acacgctttt catttttcag gctcagactg agtgcgctca
     4921 acgccaggct ttctgcactg gcgctgtacc acagcgcctg ctggtactcc tgctggctgc
     4981 gcgttcgccc aagttgttgc tgcatccgcc cggaaagcgt gatggtcacc agcatcataa
     5041 ccgccagcaa taccagcacc acgaccagtg ccattccgcg ttttggtggt aaggtgatca
     5101 tgataattgc ggcccgcgta acaaccagat gcgttcaatc tcgccccatt gcggcgagtg
     5161 cagggtcatg cgtactgcca cagggatcgc ctgcactgat gaccaactct cctgccagcg
     5221 cgtgccgtcg tagaactgca aacggagcga atccgccgga atcaactttt gcattgtcgg
     5281 cttcacactg cctgctgcgt cggtcagcgg ccaggctaac cgttcgagat aaccatcatg
     5341 aatgcggtaa ccgacggtga gcagattact gcgcggcaga cgcatcaacg gattgaccac
     5401 gccgccacgt acaaaacgca ccccttcact ctcagacgcc agcacgccag cgcccgccag
     5461 taacgctggt tcacgctggc cctgatcgcc tcttaccgga cgcggcatca tttgggtcag
     5521 atcgtgggtc agaaaactca tcgtttgctg catgaggttt agtttttgat cgtgatcggc
     5581 gacggcgcta ttcacgcgcg tgacgccgtt tgtcacctgc tgcgccatca gtgccagtga
     5641 ggcaaaaatg gctatcgcca ccagcatttc cagtaacgtg aaaccagcgc gagtccttct
     5701 cactgttggc ctcccacggc gctaaaccag gcgcgtcgtg actgaatcac cggcgaaaag
     5761 tcttcgtgaa ggctgacttc aatatccacc gcctggagca gcgcgttacc ggtattcagc
     5821 ggtgtggttc gccagtacca gcggcttcct gccatatcac tctcgccctg gtgcctgacc
     5881 gctcgttgtt cacgggtcat caggtaaagc agcgcagtct ggttttcagc tacccagccc
     5941 gctaccgttt tctcctcaag gatttgctga ttactcagcg caccgctggc agtctgtaac
     6001 accgccgtgg cagccagcgc aaaaatcgcc agcgcgagca tcacttccat caaggtaaat
     6061 ccgcgcttca tggcatcctc tcatcacatt gattgaggga taacgcgcca tcgtgcgcca
     6121 gctttaccgc ccaacatgcg ttttgcgccg ccgagtagaa gcgcagcgta aacggcgtgg
     6181 cgggttcaaa aggcgaaaaa cggatctgcg gcgtcttctt tttcgcctct ttttgcagct
     6241 ccagttcaat atcgtgcagc gtcaggcgac gacgctgcag ctccagcgta tattccttct
     6301 gccagatatc actgccgggt tgtaacagca tctgcacctg tttcggcacc gtaacctgtg
     6361 ccgataaccg ggtcgcagaa acgggcaacc attgcccgtg acgacgctgc ataaactggt
     6421 aacctggcgg gtcgattagc acaccgagtg tttgcccttc gatcactgcc ctgtccttaa
     6481 actgcgcaaa gcgagtcaga aaatcctgcg ccgctttttt cgcaggcggc tctgaagcgg
     6541 tcgcaaacgt ctgtaccacg cccgcactgg caaggccgat aaggaaaatc accagcatga
     6601 tttccagaag tgtgaatccg cgttcaggca ctgattactg aaactcctgc aaattccagt
     6661 taccgatatc tgcgccagcg ccctccccgt tttcctgacc atctgctccc agggtataaa
     6721 catcaaacag ccctttttca cccgggctga gatactgata atcattgccc cacggatcct
     6781 ttggcagtcg tttaatgtat ccaccggtac ggtagttacg ggaatccgcc atattggccg
     6841 gttgctggat cagcgcctcc agcccctgtt cggtagtcgg ataacgcccg ttatccagtc
     6901 ggtacatatc cagcgcattc tccagcgcca cgatatcgct gatggctttt tgccgatcgg
     6961 ctttctcttt gttgcccaac aggttaggca ccaccagact tgccaggacg ccaagaataa
     7021 caatcaccac catcacttcc agcagggtaa aacctgcccg tggtttttgt gtgcgggata
     7081 acgaattcat gtgataactc cgtaaattac attccaacca tattgttcag ttgcagcatc
     7141 ggctcgagga tggcgatgac gataaacagc accacgcccg ccatcatcac caccagagca
     7201 ggctcaaaca gccccaacgc cagcctcacc tgggtatcaa actcccgttc ctgattgacc
     7261 gcggcctgtt caagcatggt ttccagttca ccgctctgtt cgccggaggc gatcatgtac
     7321 agcatcatcg gtgggaacag gcgcaactcc gccagcgcag cgcgcagact gcttccttcg
     7381 cggacgcgat ctgccgccag cagcagttgt tgctcgacat aacgatttgc cgacacggcg
     7441 gcagcggtct gaatgccttc cagcagcgga acactgctgg cggtgaggat gcttaacgtg
     7501 cgggaaaaac gcgccgtatt cagcccgcgc gcaacacgcc ccgtcacggg caaacgcagc
     7561 aaggttttat cccagcgcag gcgcatggca ggatttttga gtagccgttg ccccagcacc
     7621 agaagcccca gcaatcccgc cagccagtaa acgccgctgg tctgtaacgt gtcgctcatg
     7681 gcgatgagcg tgcgggtgga ggcaggcagc gcgtgtccga gatgatcaaa ctgttcgatg
     7741 atttttggca ccactgccgt cagcaaaata gtgaccacgc ccgttgccac caccagcagt
     7801 accagcgggt agagcatggc ctgcagcagg cgtgatttca gacgctgccg ctgttcggtg
     7861 taacccgcca ggcgattgag caccacgtcg agatgtccgg atttttctcc ggcagcaacc
     7921 atcgaacaaa acagggaatc aaagacgcgg ggatgttcgc gcaggctgtc cgacagggtg
     7981 taaccttcct gaatccggct gcgcagcgcc attccgaggc tttttacatg cagtttttca
     8041 ctttgctcac tgaccgcctg taagcaggtt tccagcggca ttgctgcctg taccagcgtt
     8101 gccagttggc gcgtgaacag cgcaagatct gccgccgcca cgcgacgatg tgcgtgccgc
     8161 cgacgctgca acatcccccc tgacgaagta ttcatccggg cttcaatatg cacggggata
     8221 agctctttac cgcgcaacaa ctggcgggca tgacgcgcgg aatccgcctc aatcatacct
     8281 ttggttttgc gaccattacg ctccagcgcc tgatagtaaa acagtgccat tacgcctcca
     8341 tggttacccg cagaacttca tcgagagagg tttctccggc gagcactttc tcaatgccgt
     8401 tgctgcggat acccgcagag tgttgtcgga cataacgttc cagctccagc tccccggcct
     8461 gacggtggat caaatcacgc aatgtggcat ccaccacgat cagctcatgg atggcagtcc
     8521 gtccgcgaaa acctttgtga ttacaggcgg gacagccctg tggatggtac agagtgacgg
     8581 tacgggcgtc ggtaattccc agcaggcgtt tttcttcgtc agtggctggt gcgggctggc
     8641 ggcagtcggg acacagcgtg cgaaccagtc gctgcgccat cacgcccgtc agactggaag
     8701 agagcaggaa aggctccacg cccatatcct gcaaacgtgt gatcgccccc accgctgtgt
     8761 tggtatgcag cgtggaaagg accaggtgtc cggtaagtga agcctggacg gcgatttctg
     8821 cggtttcggt atcgcgaatt tcaccgacca tcaccacatc cgggtcctga cgcaaaatcg
     8881 cgcgcagccc acgggcaaag gtcatgccga cgcgggtgtt aacctgcgtc tgaccgatcc
     8941 cttcaatcat gtattcgatg ggatcttcaa ccgtgagaat gttgcgcgaa tggttgttca
     9001 gctcctgcaa tccggcgtac agcgtggtgc ttttgccgga acccgtcggc cccgtcacca
     9061 gaaagatgcc gtgcggtttg tgtaacagct ggcgcaactg cgcggtcagt tgctggctaa
     9121 gccccagacg ctccagcgtc aggcgggcct ggtttttgtc cagcagtcgc agcaccacgc
     9181 gctcgcccca ggcagaaggc atggtggaga cacgcacgtc aatcgcccga ccgcccagca
     9241 gcagcgcaat gcgcccatcc tgcggtacgc gtttttcggc gatatccagc cgtgccatca
     9301 ccttaatacg cgataccagc agcgaggcca gtttgcgccc cggacgcagc atttcatgta
     9361 gtgtgccgtc aacacgaaaa cggatcacca gactcttttc aaacgtctcg atgtggatat
     9421 ccgaagcgcc ttctttaatc gcctctgcca acatggcgtt gatcagtttg atgattggcg
     9481 catcgtcgtc actttccagc agatcttccg tttccggtag ttcttctgcg agagtaaaaa
     9541 agtcctcagc agaaccgaga tcttccatca gctggcgggc ctcggaagag tcccgctgcc
     9601 agaccgcatt cagccgctgt tcaaattcgg cctcgtcgat ttgcctcagc gtaaaagacg
     9661 cgttcagccc ccgctgcagc tcctgcaaaa cagagagcga caacggatga acatggagga
     9721 tctccagcga cgcttcgcac catgccacca ggctaaaccg acggctgaaa ctgtagggca
     9781 gacgcacggt gttagcggtg gtttcctgtg ctacaggcac cattaacgcg ttctcccggc
     9841 attaaggaac gcgcgaactt ctggcggtag agcctgattt tgcgctggca gtaccggctg
     9901 cgcggtgtgc ggcatcaggc ttaagccctg ctcatcgcgg tagatttgct cggcgcgcat
     9961 gtagttatat ttgcgctgcg acacaccgtc tgccgccata ccgtcacgca gaatggtcgg
    10021 gcggataaac accatcaggt tacgtttttc ttttttatcc gccgtcgatt taaacaggtt
    10081 accaatcaac gggatatcgc ccagcagcgg cactttcgcc acgctttctc ccgcctggtc
    10141 gtccatcaga ccgccaagca caattagctc accatcgtta gccaacacgg tggttttcag
    10201 tttgcgctca ccaaacacca cgtcgaggct ggtctgtcct tccaccttcg acacttcctg
    10261 ctcaatcacc atctgtaccg cgtttccttc gttaatctgc ggcgtgactt tcagcatgat
    10321 gccgactttt ttcctctcta ccgtgttgaa aggattgctg ttattggagc caacggtaga
    10381 tccagttaat accggaacgt cctggcccac catgaagaag gcttcctggt tgtccagcgt
    10441 ggtgatgctc ggcgtggaga gcacgttcga gctggagtcg tttttgaccg cctgtaccag
    10501 cgccatccag tcgcctttca ccacgccaac cgccgtaccg ctaaagccag aaagaagctg
    10561 agcaagcgtg gagagatcgc cgttagcatc cggatttatg gtggtagcgc cgttttcact
    10621 gatcaccgtg gagcctttct gcggttttgc ctgagaaatc gctgcgccca gcgtaccaat
    10681 agggatctgc gtaccgttag caaactgcat taatccggca tctttcgacg cccactgcac
    10741 gccgaaattg atattactgc cttcggcaac ttccacgatc aacgcctcga catgtacctg
    10801 agcacggcga atatccagtt gttcaatcac gctttgcagc gactgcatga tgtcctgcgg
    10861 cgcggtaaca atcagggcat tactgtgttt gctggcggcg atggagacaa cctcacgccc
    10921 gctaccgacc gtgccttccg cctcttcttt agccgccgtg agcgtgccgc tgacctgctt
    10981 cagcacatcg accagatctt cggctttgct gtatttgaga tagaaaacct ggctgttgcc
    11041 gctgcgttcc atttctgagt ccagccgacg gatcaggcgg cgcattttgt cccgcgtggc
    11101 cgggtcacca ctgacaatca cactgttggt gcgttcgtcg gcgacaattt gagatttcag
    11161 cgtcgcaggc tggttctcgc cgctgttttt agtcaggctt tccagcacgc gggcgatttc
    11221 cgaagcagag gcgttatcca gcgggatcac ctcttcagtg cgattacccg cgtgatccac
    11281 acgctggatc acttccgtca gccgctccac gacggaggcg cgcccggtga gcataatcac
    11341 gttggaggga tcgtaattaa caacgttgcc tgagcctgcg ctgtcgatca tctggcgcag
    11401 aatcggtgcc agttcgcgta ccgaaacatt acgtaccggc acgactttgg tgaccatttc
    11461 atcgcccgcg tagttgtcgc tgccttcacc aaccagcggc agcggctcga ctttcgcggc
    11521 actggatttc accaccttca gcacgtcgtt ttccatcggt acgaccgcat acccctgtgc
    11581 ttccagcagg ttaaggaata gctggtaata ctggcgttca ttgagcgggg tcatggtgcg
    11641 aatactcact ttcccctgta cgcccggccc cataatgatg gttttattaa ggttagcgcc
    11701 gacggtttcg atgaacgatt tcaggtcggt atctttaaaa ttagcggtga aagtggcttc
    11761 ttccgcccag accggtgaac tgcataatgc cgctgccagc accagcggca gtaaacgctt
    11821 ttttgttttg aggccagttg tcttcttacg ccagaccgac aacgtcatat cacgccaaaa
    11881 cacgatgaat gattctcctg gattaaatgc ggttagcgca gcgcgatgga aatgtcgtgg
    11941 cgcgcaccct tgcgtaaaac cgtaagttga atggaatcca ttgaaggtaa ctgccgcatc
    12001 agagcaatca ttgctcgtgg atcagtgaaa tcctgctgat ttagcgcaat ggcgatatcg
    12061 ccttccttaa aaccgctggc atcgaacaga gaacgatctg cccccggttt cactgcataa
    12121 ccgacaatcc cttccttacg cacaggcgta agctggatat agttaaaaat tttctgcgga
    12181 tctttcgcca gtgcctgacg cacggcagcc gggatctcca ctggcgcact ggcagcaggc
    12241 tcagcaacag cttgctttgc ttcgtcgctg acggcttttt tgctggtcac ggctatggtg
    12301 ggacgcttct cttctgccag actcagacgt tccattttcc cctgatagcg cagcatcaca
    12361 tggtcgcggt tgatttcctc aatcactgcg ttgtgagagc caagccgttc accctgcaaa
    12421 tagacctgct gtttgccgcc ttcttcaata accgcgccgg gtctggcacc aaaggcgatc
    12481 ccgcgcagca ccacattaag acgcgtttct gccacaggcg cagattcagg ttgttttacc
    12541 ggtgcggcga cgggttgata tttgccaaac cagttttgtt ggctgactaa ttgcatgtca
    12601 tttttatcga acggttttgc atccgcacgg agcggtttat tcgccgatgg ggaaaccgcc
    12661 gtatattccg cagaaaagga gaaatagcgc cagagtgaat gcgccacttt tgcagaaata
    12721 ataagcagca tcagccagaa catcccgcgt gcaattttgc gcagatgctc tttgtctgta
    12781 ttaagagatt gtctctggtt aagagtgtga cgaatttttg tcagccattg aatgagataa
    12841 attcgtgcgt cacgaaaaac aacccgcgcc aatttatatc tacccgacgt tatgctttga
    12901 ctataccaca ggtggtacga tccagtttcc gctgatactg gttacctgta cgtgtatcat
    12961 taatcgttat gctgtaatta attccctcag tgatcattaa tttcaccgtc gggtcagcgc
    13021 acatttgtcg ttgatagctg gttaaaaagg cgtcaggtgt ctgcgtggtc tgagtacccg
    13081 cttcgctgat aatggtcatt tttaccgtcg tgccgctact ttgtgccagc actaaggtat
    13141 atcccgcaga tttaatcggc aggttttggc tgatattttg cgcctgtttt ttcgccagta
    13201 aactggcgtt ttcattatgg ctggcgcagc cacttaataa tcctgtaacg ctcaacaata
    13261 gcgatattaa taccctccct ggcatctgtt ttatcgacat ggtaaataat ctctaaggtt
    13321 attaataaga gttaaaatgt cactttgata atgacgtggt tatcattaaa acaatgcctg
    13381 tagataaagt gttgctatac cgcccagact taaacacggt ccaaaaggca gtgtggttga
    13441 tcctcttttt gtaataacgg catatatcag accgcagcat gatgcgatta aagcaacatt
    13501 gggtagcgac aacggcccca cccagccacc taaagcggcg aaaagtaata catcgcccat
    13561 gcctaatgct tctttacgca gaactattcc ggctatccag cgcagggagt aaaaagtgat
    13621 aaaccccacc aggacgccgg taactgcgtc ttgtagcgtt aacggactct gttgcgccca
    13681 tgccgcaatc agcccggtcc acaatacgcc ctgagtaaaa acatcgggca gccattggtt
    13741 atcgaggtca atgacgctcg cggcaatcag ccaggcggat aatatcatca ccgccagccc
    13801 ccatccactt tctggccaca ccagactcgc cagcaaaaaa gcgagtgccg tcaataactc
    13861 caccaacgga taacgcttgc tgattttcgc ctgacaatca cggcagcgcc ctttgagcat
    13921 caaccaggag agcagcggaa tattgtcacg aacgcggata gtctgctggc aatgcggaca
    13981 gtgcgaacgc ggcagcgcaa ggcttatttt tgactgcgca ctcgacattt caccgtgaaa
    14041 ctccgccatt tgttggcgca gcatgatggg gtaacgccaa atcaccacat tcaaaaaact
    14101 gccgatgatc aatcctccga cggttgccag tatgggcatc gccgcagggt attgctgaaa
    14161 aacatcaaaa agcatggtta aaggttattt gttgtaactt gccggatgcg ggcctgcggg
    14221 tgtatgccat atggctttcc tccaggcccg ataaactggc gcatcgggca tatgtgttga
    14281 cccgatgcgc cttatttcat gccggatgcg gcgcaagcgc cttatccggg ttattcagca
    14341 ggcatagaat attcggtaac cttattaatg gtttccggcc cctgttccgg tttcggcagc
    14401 ttgagcgacg ccagcgtgtt gtatgccgac tggctcacac cgccctcgaa gctcatctct
    14461 gtcgctcccg gcaactggta agcattcgcg cccggattcc atttcttaaa gaactccgaa
    14521 agatccgtct gggcgaccca ggaggcacac agcatcagcg tgtcggcagt gttaccgttg
    14581 gattcggcac agtaatttct ttctccaaac ttggttttgc caacctcatc gccgcgcgct
    14641 ttacggtgca tcaactggaa caggttccag cctttcatcc cttcacgctc gctgtagaac
    14701 tctggcagag agccttctgg ataccactgt ttgatatcaa agtttttctc tgcccattcc
    14761 ttcagctgcg cgtacatcag cagacggtca cccgcaccgc cgcgcgccca tgcctgaccg
    14821 ttgctctctt ccagatattc cggcgcgacg gtaatgtcgt cagcgacacg gttcatcttg
    14881 ccgagatagc gatcctgcat gtacagcgcc agcacgttgt tcgctacttc agttgcgcca
    14941 ggaacagtca gcggcgtttc ggcggcgttg tgaccaactt cgtgccagat cagccagtcg
    15001 ttcagcggcg tcgtcggcag cgtggtgctg ttcgtcgaga agctgctgtt cattaccgga
    15061 taaccagagt gcgcatcacc gatggagatc tgcacatcgt tggtgaaacg atgcttgtgc
    15121 cccgtcaagt ttttataggt aaacatccgg tgcttaccgt tttcgccatc acgtccgtag
    15181 aagtcattca tcgagctggc aaaggtatcc agttcctcag cgaattgttc ctgaccgccc
    15241 ttgtaattgc tggcctcaag gttcttcttc ggcgtggtgt agacgaaagc gtctgactcc
    15301 agctcgccca acggcgcagg ggagttcaga gcgtttttcc atgcgccatc tttatagaac
    15361 ggcgctttca ccacaccagt aaaggtgaat tcggctgact cattctttgg gctgttgccc
    15421 ttgatataaa tcagaccacc gtaaggaacc gtaaacttca cctcaccatt ggctttcagc
    15481 tcataggttt tcgtcacttt tggcggacgg ttcagagcaa cttcatgctt ctcacgtccg
    15541 gtcaggtcgt cggccagcgc cacggtgaca gtcacaggaa cgttcgcatt ggacttaatg
    15601 gtgacctctt tctgagccgg tgcccacagg ccagttgact gcatgttacc tgcaaaccat
    15661 ttggtcggat tcgagtacag gctgatggtt tcagtaacct tctcaccttc tgccgatacc
    15721 gctcccggat acttctcgac atcaactttg atgttcagat cccaccagga acgacccagc
    15781 atcaggcgcg tcagcggttt ttccatatag ttgagcggat agctcgggtt catcatgccc
    15841 gctttattaa cgctcttctc gccgtagatc atgttgttat cgaccagcga ttttttcagc
    15901 tcatcagaac actgcgtgcc gccagtatag gcatcgttgg cgtagcagtt caggaactcg
    15961 gtgaacgttt taaagcccag ctcgtcatcc ttgtcgtttt catagcgata ttcaatttta
    16021 ttccacagcc agaccgacat gttctggtac agacgttcca gatcgacgct gctcagacgc
    16081 tcacctttgc gaccattggt ccggaagtag agctcatgct gatacagacg ctgaatgttg
    16141 gtgcctaaat ccgcagcctg caccatcgct tttgccgtgt cggcgttaag gcttagttgc
    16201 gtatactgtg gaacatacat gccaccagta accggaaccc ccgtgccagg acgatattcc
    16261 agacagttga cctcgtagtg gtaagttggg tccttacact cctttaatcc aggaaacttc
    16321 tcaaagattt ttgccttcgc agccttcaga gaatcctctg ttttatgatc ggcctcatca
    16381 ataaaggcat aacgcgtttc ctgtttgcca tctacatctt ccagccagct ggcaacttcc
    16441 agcttcggtt tgtcatcagg tttgttttct acctgatatt tccacttaac ttcccctgtc
    16501 ttactatcga tggtgtacgg cagcgcacca tctacggcag gataacgttc atagacccaa
    16561 atgcccgttg cgcgctgctg acgaacgcgg ttcggatacc cttgcggatc gttatttact
    16621 accgacttgt tcagtgccat cgacagacct gcggcatcaa gcagacgtac aaagccagac
    16681 gcgctctctt ccttaagatt gctcatcacg ttttccatga tcagcaccga tccacctttg
    16741 ttcagatagg cgatcagatc ggtcacatcc tgctgagtca gcttcggttt gctggtatct
    16801 gctcgcagcg ggattgcata aggatcgtta ccaacctgag tcacatactc aaagccgttg
    16861 aggatcagca gcggcatttc ctgcgggtcg agatcaccat agctacttaa atgctcaaca
    16921 gagatacccg caaaatccgg atgaaagccg aactcagcgc tgtttcctgt aacctgacca
    16981 tgacgtttga aatagacagt atccaggttg gtgcctacgg tcatgctggc tttcgcgtcc
    17041 ggcgtccatt tatcgtcgga cagatagcgc aacacgttct gcataaagtg cttcatatca
    17101 ttagaatcac tgttacgcgt acactgaccg tttttatcaa caccgccttc ccagctgtaa
    17161 ccgttcggac aacgcaaaat gctgttgtag tgcgggttac cgataaccat cagtttgccc
    17221 tcaccgactt gccccagcga aataaacggc aggttaaagg tggcggtatc gcgcgtaacg
    17281 ttttccggct caacgagaga aggcgcttcc gtaatgtacg ccagctcgtt tttatcccag
    17341 gcgcgttttt cgccaaacgc cagccagtag tttttatcat tacgcgccat cagaatcggg
    17401 aaggccgcgt tggagatatt caccactgcc tgaccgcgcg cattaccggt actgccatag
    17461 aagttggtag agtcatggaa gacgtggaat ttcgtcactg atttgtaatc tttatccaca
    17521 ccccacagct tgttgataac gccctgaatc tgaccttcgt taacattgcg tgctgtcaac
    17581 gagaaccaac gctgagagtt gtagccccca agggagtcac aaatcgccgt atcaatctct
    17641 ttagcctgcc cggattcaaa ctgctcaaga aattcgtttg tccgctcaaa ggtttgatcg
    17701 ccttcgctca acgcctcgcc attggataac gagagattga taatctcgtt gattacgttg
    17761 ggatactcgg caaagacctt gcgcactacg tccggcactt cacgctcatc attttttccg
    17821 gcctgggagt aacgatgaat aagctgatca atattcgcgc cgcgaacttc gtcacccagt
    17881 tcagtcaacg caatggtcga cttattgccg cgcactgagc ccagttcaaa ggtgtcgata
    17941 ccaaaggaga tggtttcgcc ccagctgaag ttgaatttgc cgttttcgcc agttacgcca
    18001 cggcctgagc tggtgtagta atttacgcca accacaccat tgcccatgct atccaccagt
    18061 cggccttcgg aaagaatgat ttcagtgggt tgatactgat aaaactgttc cgcgttagcc
    18121 gacacgaagg acgcgttcag atccggtttt gttcccggcg tggtgacagg cacgaccggt
    18181 gaggtatgag tggatggcgc tttatcggtc gcagcattgt tttccacctc ttcattgacc
    18241 agctttttga actcttccgg tgccagatcg atttgcttat acagcgagtc gaaacgttta
    18301 ctctcgatca ccgaggagaa cgtcaggcaa acctgttctg tatccgccgg acagctgtta
    18361 ctggacgtta ccagcgaaac cgcattgctt ttcttgtcat cggaagctgc cagttcttgc
    18421 gcgtcctcaa gactaaacga cactttttca accgcacgca ggctacgcgc agcttctgac
    18481 tgggtgtcga aggtagcaat tgtcgtgttg ttccctgcca cacaggtgac tttgtcgcct
    18541 ggcgtaaagg taaagccatc gctggattca ccattacaag tagcaccagt tatccgctgg
    18601 cttccgccca gggtcagata acccgttttc gtaggaacag gttctggctc cggttctggt
    18661 gttggctcag gatcaggtgt cggttccggc gtaggttctg ggtccggcgt tggctcaggc
    18721 g
//
LOCUS       DAYPHN010000091        18606 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000091 DAYPHN010000000
VERSION     DAYPHN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.091"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            7..546
                     /gene="rplE"
                     /locus_tag="ACG0BW_002964"
     CDS             7..546
                     /gene="rplE"
                     /locus_tag="ACG0BW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFK7259899.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            561..866
                     /gene="rpsN"
                     /locus_tag="ACG0BW_002965"
     CDS             561..866
                     /gene="rpsN"
                     /locus_tag="ACG0BW_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFK7259900.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            900..1292
                     /gene="rpsH"
                     /locus_tag="ACG0BW_002966"
     CDS             900..1292
                     /gene="rpsH"
                     /locus_tag="ACG0BW_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFK7259901.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            1305..1838
                     /gene="rplF"
                     /locus_tag="ACG0BW_002967"
     CDS             1305..1838
                     /gene="rplF"
                     /locus_tag="ACG0BW_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFK7259902.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            1848..2201
                     /gene="rplR"
                     /locus_tag="ACG0BW_002968"
     CDS             1848..2201
                     /gene="rplR"
                     /locus_tag="ACG0BW_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFK7259903.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            2216..2719
                     /gene="rpsE"
                     /locus_tag="ACG0BW_002969"
     CDS             2216..2719
                     /gene="rpsE"
                     /locus_tag="ACG0BW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFK7259904.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            2723..2902
                     /gene="rpmD"
                     /locus_tag="ACG0BW_002970"
     CDS             2723..2902
                     /gene="rpmD"
                     /locus_tag="ACG0BW_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFK7259905.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            2906..3340
                     /gene="rplO"
                     /locus_tag="ACG0BW_002971"
     CDS             2906..3340
                     /gene="rplO"
                     /locus_tag="ACG0BW_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFK7259906.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            3348..4679
                     /gene="secY"
                     /locus_tag="ACG0BW_002972"
     CDS             3348..4679
                     /gene="secY"
                     /locus_tag="ACG0BW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFK7259907.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            4711..4827
                     /gene="rpmJ"
                     /locus_tag="ACG0BW_002973"
     CDS             4711..4827
                     /gene="rpmJ"
                     /locus_tag="ACG0BW_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFK7259908.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            4974..5330
                     /gene="rpsM"
                     /locus_tag="ACG0BW_002974"
     CDS             4974..5330
                     /gene="rpsM"
                     /locus_tag="ACG0BW_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFK7259909.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            5347..5736
                     /gene="rpsK"
                     /locus_tag="ACG0BW_002975"
     CDS             5347..5736
                     /gene="rpsK"
                     /locus_tag="ACG0BW_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFK7259910.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            5770..6390
                     /gene="rpsD"
                     /locus_tag="ACG0BW_002976"
     CDS             5770..6390
                     /gene="rpsD"
                     /locus_tag="ACG0BW_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFK7259911.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            6416..7405
                     /gene="rpoA"
                     /locus_tag="ACG0BW_002977"
     CDS             6416..7405
                     /gene="rpoA"
                     /locus_tag="ACG0BW_002977"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFK7259912.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            7446..7829
                     /gene="rplQ"
                     /locus_tag="ACG0BW_002978"
     CDS             7446..7829
                     /gene="rplQ"
                     /locus_tag="ACG0BW_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFK7259913.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            7936..8304
                     /gene="yhdN"
                     /locus_tag="ACG0BW_002979"
     CDS             7936..8304
                     /gene="yhdN"
                     /locus_tag="ACG0BW_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFK7259914.1"
                     /translation="MWLLDQWAERHITEAQSKGEFDNLPGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYKEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            8315..8740
                     /gene="zntR"
                     /locus_tag="ACG0BW_002980"
     CDS             8315..8740
                     /gene="zntR"
                     /locus_tag="ACG0BW_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFK7259915.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            8796..9014
                     /gene="arfA"
                     /locus_tag="ACG0BW_002981"
     CDS             8796..9014
                     /gene="arfA"
                     /locus_tag="ACG0BW_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFK7259916.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(9011..9421)
                     /gene="mscL"
                     /locus_tag="ACG0BW_002982"
     CDS             complement(9011..9421)
                     /gene="mscL"
                     /locus_tag="ACG0BW_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFK7259917.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(9551..10927)
                     /gene="trkA"
                     /locus_tag="ACG0BW_002983"
     CDS             complement(9551..10927)
                     /gene="trkA"
                     /locus_tag="ACG0BW_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFK7259918.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(10949..12238)
                     /gene="rsmB"
                     /locus_tag="ACG0BW_002984"
     CDS             complement(10949..12238)
                     /gene="rsmB"
                     /locus_tag="ACG0BW_002984"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFK7259919.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDACYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     VLPGFEEGWVTVQDASAQGCMAWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLIYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(12284..13231)
                     /gene="fmt"
                     /locus_tag="ACG0BW_002985"
     CDS             complement(12284..13231)
                     /gene="fmt"
                     /locus_tag="ACG0BW_002985"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFK7259920.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKALPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(13246..13755)
                     /gene="def"
                     /locus_tag="ACG0BW_002986"
     CDS             complement(13246..13755)
                     /gene="def"
                     /locus_tag="ACG0BW_002986"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFK7259921.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            13885..15009
                     /gene="dprA"
                     /locus_tag="ACG0BW_002987"
     CDS             13885..15009
                     /gene="dprA"
                     /locus_tag="ACG0BW_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFK7259922.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSYIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            14981..15454
                     /gene="smg"
                     /locus_tag="ACG0BW_002988"
     CDS             14981..15454
                     /gene="smg"
                     /locus_tag="ACG0BW_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFK7259923.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            15483..16025
                     /gene="yrdD"
                     /locus_tag="ACG0BW_002989"
     CDS             15483..16025
                     /gene="yrdD"
                     /locus_tag="ACG0BW_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFK7259924.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            16030..16602
                     /gene="tsaC"
                     /locus_tag="ACG0BW_002990"
     CDS             16030..16602
                     /gene="tsaC"
                     /locus_tag="ACG0BW_002990"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFK7259925.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSEIAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            16607..17425
                     /gene="aroE"
                     /locus_tag="ACG0BW_002991"
     CDS             16607..17425
                     /gene="aroE"
                     /locus_tag="ACG0BW_002991"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFK7259926.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            17422..17679
                     /gene="yrdB"
                     /locus_tag="ACG0BW_002992"
     CDS             17422..17679
                     /gene="yrdB"
                     /locus_tag="ACG0BW_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFK7259927.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLL"
     gene            complement(17655..18209)
                     /gene="yrdA"
                     /locus_tag="ACG0BW_002993"
     CDS             complement(17655..18209)
                     /gene="yrdA"
                     /locus_tag="ACG0BW_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFK7259928.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQSNQTQP"
BASE COUNT         4676 a         4443 c         4778 g         4709 t
ORIGIN      
        1 agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa actcatgact
       61 gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac cctgaacatg
      121 ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc agacctggca
      181 gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc aggcttcaaa
      241 atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg catgtgggag
      301 ttctttgagc gcctgatcac tattgctgta cctcgtatcc gtgacttccg tggcctgtcc
      361 gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca gatcatcttc
      421 ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac cattaccact
      481 actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt cccgttccgc
      541 aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa gtaaaacgcg
      601 tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc atctctgatg
      661 tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact ctgccgcgtg
      721 attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgtccg catggtttcc
      781 tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc ggtgaaatcc
      841 cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag taaagacaga
      901 tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt caggccgcga
      961 acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc aacgtgctga
     1021 aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct gaactggaac
     1081 ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt gtcagccgcc
     1141 caggtctgcg catctacaaa cgtaaagatg agctgccgaa agttatggcg ggtctgggta
     1201 tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc caggctggtc
     1261 ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct cgtgttgcta
     1321 aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag gttattacga
     1381 tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa gttaaacatg
     1441 cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg gcacaggctg
     1501 gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc ttcactaaga
     1561 agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg attaacctgt
     1621 ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact gctgaatgtc
     1681 cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca ggtgatcggc caggttgcag
     1741 cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg caagggtgtt cgttacgccg
     1801 acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg gataagaaat
     1861 ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg ggcgcaactc
     1921 gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca ccgaacggtt
     1981 ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa ctgaagtaca
     2041 ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc gctctggaaa
     2101 aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt cgtgtccagg
     2161 cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt gtaagatggc
     2221 tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa accgcgtatc
     2281 taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag ttggcgatgg
     2341 taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag cgatccagaa
     2401 agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg gcactctgca
     2461 acaccctgtt aaaggtgttc atacgggttc tcgcgtattc atgcagccgg cttccgaagg
     2521 taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg gggttcataa
     2581 cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg caactattga
     2641 tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta aatccgttga
     2701 agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa cccgcagtgc
     2761 aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ctgggtctgc gtcgtattgg
     2821 tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg cggtttcctt
     2881 catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg ccgaaggctc
     2941 caaaaaggcg ggtaaacgcc tgggtcgtgg tatcggttct ggcctcggta aaaccggtgg
     3001 tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg gtttcgaggg
     3061 tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc gtaaagcagc
     3121 gattacagcc gaagttcgtc tgtctgacct ggctaaagta gaaggcggtg ttgtagacct
     3181 gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga aagtgatcct
     3241 ggctggcgaa gtaactactc cggtaactgt tcgtggcctg cgtgttacta aaggcgctcg
     3301 tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg gctaaacaac
     3361 cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc agactgctgt
     3421 ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc cctggtattg
     3481 atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt gaaatgttta
     3541 acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg atcatgccgt
     3601 atatttcggc atcgatcatt atccagctgc tgacggtggt tcacccaacg ttggcagaaa
     3661 ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgc tacggtactc
     3721 tggtgctggc aatattccag tcgatcggta ttgctaccgg tctgccgaat atgcctggta
     3781 tgcaaggcct ggtcattaac ccgggctttg cattctactt caccgctgtt gtaagtctgg
     3841 tcacaggaac catgttcctg atgtggttgg gcgaacagat tactgaacga ggtatcggca
     3901 acggtatttc aatcattatc ttcgccggta ttgtcgcggg actcccgcca gccattgccc
     3961 atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg ttggttgcag
     4021 tattagtatt tgcagtgacg ttctttgttg tatttgttga gcgtggtcaa cgccgcattg
     4081 tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag agcacacatt
     4141 taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc agtattattc
     4201 tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac tggctgacaa
     4261 caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat gcgtctgcaa
     4321 tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa acagcagata
     4381 acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa acggcgaagt
     4441 atatcgataa agtaatgacc cgcctgacct tggttggtgc gctgtacatt acctttatct
     4501 gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc ggtgggacct
     4561 cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa actctgatga
     4621 tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac ggccgataat
     4681 tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt caagaaatta
     4741 tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg cagtgccgag
     4801 ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca aagttgggtt
     4861 gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat ttgagtatcc
     4921 tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc atagtggccc
     4981 gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta acttcgattt
     5041 atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc gctgaagatg
     5101 ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa gttgccaaat
     5161 ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc ctgatggatc
     5221 ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt cagcgtacca
     5281 agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa tcggggtgat
     5341 tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca agtctctgac
     5401 ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac tgatcgtcag
     5461 ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc tcgcaaatcc
     5521 actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa agaatacggc
     5581 atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc tactattcgt
     5641 gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc gatccctcat
     5701 aacggttgtc gtccgccgaa aaaacgtcgc gtataacgct tcgttttcca ggtttgttgg
     5761 agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg tgagggcacc
     5821 gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct
     5881 cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa
     5941 aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa
     6001 gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct ggaaggtcgt
     6061 ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc acgtcagctg
     6121 gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt
     6181 agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc
     6241 gctctggagc tggctgagca gcgtgaaaag ccaacctggc tggaagttga tgctggcaag
     6301 atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga cattaacgaa
     6361 cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg acacaatgca
     6421 gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag tgagttcgac
     6481 gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc tgggtaacgc
     6541 actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg ttgagattga
     6601 tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc tggaaatcct
     6661 gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta ttcttacctt
     6721 gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg gtgatgtcga
     6781 aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt ctattagcat
     6841 gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa ttcattcgga
     6901 agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc ctgtggagcg
     6961 tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg acaagctggt
     7021 catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc gtgcggcaac
     7081 cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc agcctgaagt
     7141 gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg atctggaatt
     7201 gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg gtgatctggt
     7261 acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat ctcttactga
     7321 gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcctgg aaaactggcc
     7381 accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact gagaaggata
     7441 aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc catcgccagg
     7501 ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag acgactctgc
     7561 ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc aagactgata
     7621 gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga taacgagatc gtggcaaaac
     7681 tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact cgtattctga
     7741 agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg gttgatcgtt
     7801 cagagaaagc agaagctgct gcagagtaat ctgaagcaac gtaaaaaaac ccgccccggc
     7861 gggttttttt atacccttag tatccccact tatctacaat agctgtactc tttttgttca
     7921 tcccctggag tatttatgtg gttacttgac cagtgggcag agcgtcatat tactgaggca
     7981 caatccaaag gtgagtttga taatcttccg ggaagtggcg agccattgat tctggatgat
     8041 gattcacacg tgccaccaga attacgtgcg gggtatcgct tgctgaagaa tgccggttgc
     8101 ttaccgccag aacttgagca gcggagggaa gcaatacagc ttctggatat cctcaaaggt
     8161 atccgtcatg atgatccgca atataaagag gttagtcgtc gattgtcatt actggaattg
     8221 aagctgcgac aagctggatt gagtaccgat tttttacgcg gcgattatgc tgacaagttg
     8281 ttgaacaaaa tcaacgataa ctagtggagt atgtatgtat cgcattggcg agctggcaaa
     8341 aatggcggaa gtaacacccg acacgattcg ttattacgaa aagcagcaga tgatggagca
     8401 tgaagtacgc accgaaggtg ggtttcgcct gtacaccgaa agcgatctcc agcgattgaa
     8461 atttatccgc catgccagac aacttggttt cagtctggag tcgatccgcg agttgctgtc
     8521 gatccgtatc gatcctgaac accatacctg tcaggagtca aaaggcattg tgcaggaaag
     8581 attgcaggaa gtcgaagcac ggatagccga gttgcagagt atgcagcgtt ccctccaacg
     8641 ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt tattgctcga ttcttgaagc
     8701 tcttgaacaa ggggcgagtg gcgttaagag tggctgttga ttttttacac tggcaggatt
     8761 acactcgcgc cgttaaataa ccaattggag tttttatgag ccgatatcag catactaaag
     8821 ggcagataaa ggataatgcg atagaagcat tactacatga tcccttattc cgacagcgcg
     8881 tagagaaaaa taagaaaggg aaaggcagtt acatgcgaaa aggaaaacat ggcaatcggg
     8941 ggaactggga ggccagtggc aagaaagtaa atcacttttt taccactggc cttctgcttt
     9001 caggcgcttg ttaagagcgg ttattctgct ctttcagcaa atcacgaatt tctgtcagta
     9061 atacttcttc tttagttggt gcaggcgcgg ctgctggttc ttcttttttc cgattcagtt
     9121 tgttgattag cttaatcgcc ataaagatgg caaaggctac aatcagaaaa tcaaagacgt
     9181 tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat atccccctgc gcatcgcgta
     9241 gcgtgacagc aaactgtttg aaatcgatcc cgccaattaa taagcccaga ggtggcataa
     9301 tgatatcggc aaccaatgaa gatacaatct tcccgaatgc cgcaccgata atgacaccca
     9361 ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg aaattcttta ataatgctca
     9421 tgttattctc cctatgaagc cgacaatcat aagtctaaca aatgttaagc tattttcctg
     9481 ccaggaatgg atttaattaa tccttgataa tcaaagggaa ataataaggc gtcattagac
     9541 gccttattaa ttacaagaag aaaggacttg gctggaagag tctttcgacg tcggtaataa
     9601 actttttatc tgtgaggaac ataattacgt gatcgccttg ctcaatgcgc agattgtcat
     9661 tggcaatcat cacgtcgttt ccacgtacca ccgctccaat aatcgttcct ggcggtagct
     9721 tgatttcatc aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg tgagcaacgg
     9781 cttcaatagc ttctgctacg ccgcggcgta atgaggaaac accaacaata tctgcttttc
     9841 gcacatggct aagcaacgca gaaatagttg cttgttgcgg tgaaatcgca atatcgatca
     9901 cgctcccctg aaccagatcc acataagcgc gacgctggat caataccatc acctttttcg
     9961 cacccatacg tttggcaagc atggcggaca tgatattggc ttcgtcatcg ttggtgacgg
    10021 caataaacag atcaacttga tcgatatgtt cttcggccag cagttcttga tccgacgcat
    10081 caccaaaaaa gacgatcgta ttctgcaact tttccgccag ttcggcagcg cgctgctgat
    10141 tacgttcgat gagtttgacg ctgtaatctt tttccagacg acgcgccagc cctgcaccga
    10201 tattaccacc accaaccagc atgatccgct tatacggttt ttccagtcgc tgtaattcac
    10261 tcatcaccgc gcggatatgc tgtgaagcgg caataaagaa cacttcatca ccagcttcaa
    10321 caatggtcga accttgcgga cgaatggggc gatcatggcg gaaaattgct gccacacgag
    10381 tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc agcgggccgc
    10441 cataataggc tttaaccaca gccaggctga ctttaccctc agcgaagttc accacctgca
    10501 atgcgccggg atactcaatc agtcggtaaa tattatcgat aaccaactgc tctggtgcga
    10561 tcagatgatc aatcggtaca gcatctgaat gaaatagctt atcggcatcg cgcacgtagt
    10621 ctggtgagcg gatacgagcg atgcgattag gggtgttgaa aagtgagtag gctacctggc
    10681 aggcaaccat attggtttca tctgaactgg ttacagcaac cagcatatcg gcgtcgtcgg
    10741 cacctgcctc ccgcaatacg cgtggatgag agccatgccc ctgcacgact cgcaggtcaa
    10801 atttatcctg caaggtccgc agacgctcac cgttggtatc gacaacagta atatcgttgt
    10861 tctcgccaac caggttttcc gccagtgtgc cgccaacctg gccggcaccc agaatgataa
    10921 ttttcatcag tcgcgacccg ttatctcatc actttttgat tagcttagcg taaaagaagc
    10981 cgtcgccctc ttcggcacca ggcagatttt gtttacccgg ttgctctggt gttcctgttt
    11041 cgcaaagttc ggcatcagcg gtacgttgca aaaaggcttt aatctgcagg ctattctctt
    11101 ccggtaacac cgaacaggtg gcatagatca gggttccacc ggattttaaa tgcggccaaa
    11161 tggcgtcgag aatttcagac tgcaattgcg cgagttccgg gatatcgcga tcgcgacgta
    11221 accatttaat atccggatgg cgacgaatca caccggttgc tgaacaaggc gcatctaata
    11281 aaatgcgatc aaactgttgc tcgccacacc attgggaagg gtagcggcca tcaccttgtt
    11341 tcacggtcgc cttcatacca aggcgtttta aattgtcgta aacgcgagag aggcgctgtt
    11401 cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac ctcaaggata tgcgttgttt
    11461 taccgccggg ggcagcgcaa agatccagaa tgtgttcacc gttttgtggc gcaagccagg
    11521 ccatgcaacc ttgtgctgat gcatcctgaa cggtaaccca tccttcttca aaacctggta
    11581 gcacatgaac aggtgcaggt gtttccagcc gtacagcatc agggtaatcc gcatgcggga
    11641 aacctttcat tcctgcttca tccagcaatg caagccagct gtcgcgggaa tgatgcgtac
    11701 gattaacgcg cagccacatt ggcggacgct ggttattggc ttcaacgatg gattgccatt
    11761 gctctggata cgctttttgc agacgtttca gtaaccagga aggatgcaga taacatgcat
    11821 cactggcatt aaactcggct aataactctt cttgctgacg ttggaactgg cgtaacacgc
    11881 cgttaatcag ccccttaagt tgcgggcgct taattgcgac agcaccttca accgtttcag
    11941 ccagcgcagc atgaggtgga atgcgggtat aaagcagttg atacaaacca accataatca
    12001 ggtaatgcac ggtccgctgt ttgccggtca tcggacgggc cattaactta ttaatcagcc
    12061 agtctaactg cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga agtgctttgt
    12121 ctttatcgga aactttttgc tggagcggtg gcagaatgtt gcttaatgat tgcccttgct
    12181 cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa attacgttgt tttttcataa
    12241 gtataaaaat gcccggcaag accgggctta gaagagtgga ctatcagacc agacggttgc
    12301 ccggaacaaa ccattcccga cgagaattca ggaggtcttg cgcgctcatc gctttcttac
    12361 ccgcaggttg taacgagagc aggttcagga tgccatcacc agtcgcaacc tgaatgcctt
    12421 gtttattggc ttcaaggatc gttcctggtg cagcgttggt tgccgtatca atgaccgacg
    12481 ctttccagac tttaaccggc tgtccttcaa tttccagcca gctcattggc catggattga
    12541 aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga ccagtcaata cgcgcttctt
    12601 ctttactcaa cttttcggcg taagtgacaa gagtttcgtc ctgaacttct ggtttcgccg
    12661 tgccgtctgc cagttgtttc aatgtggtga taagcccttg tgggccaagc tctgccagct
    12721 tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg gcaggagagc ttatagagca
    12781 tgtcaccggt gtctaaacca acatccattt gcataatggt cacaccagtt tctgcatcac
    12841 ctgcccatag tgagcgttgg attggtgcag caccgcgcca gcgtggcagc agtgaaccat
    12901 gaacgttgat acagccaaga cgcggcatct ccagcactgc tttaggcaga attaagccat
    12961 aggcgacgac aaccataaca tcagcctgta gatcggcgac cagttgctgg ttttcttgtg
    13021 gacgcaggga aaccggttga aaaacgggca gagctttttc ctcagccaga actttaaccg
    13081 ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg aacacgccaa
    13141 cgacgttatg accagaagac aacagcgcgt cgagatgacg cgctgcaaag tcaggtgtac
    13201 ccgcaaaaat aatacgtagt gattctgaca cgttagttct tatccttaag cccgggcttt
    13261 cagacgatcc agtttttcaa ctttctgacg aatacgttgt tgtttcagcg gtgacagata
    13321 atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga tggctaacag
    13381 accgtctgct tccagttcaa atggtttacc gtcgcggtcc agggcgcgaa ttttaacttt
    13441 ctctgcgcgt ggcactaaag cacgttgttc agggatcgac aggcaacctt cttcaatgcc
    13501 tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt cgtcacggtt
    13561 ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg ccgccaggcc
    13621 aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct gaatttctgc
    13681 attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa tatgtaacac
    13741 ttgcaaaact gacataaatc tccagagatg tgttcaggag ttagaaagat tatttcttct
    13801 attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa gattgctaag
    13861 gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg tttaatgagc
    13921 atcagcagct tgtacggcga tgatatggtc cgtatagctc actggctggc aaaacagtcg
    13981 tatattgatg cggttgtatt gcagcaaaca gggcttacat tgcggcaggc acaacgcttt
    14041 ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt ggttggagca acccaaccat
    14101 catttaatcc ctgcggacag cgaattttat cctcctcaac ttcttgcgac gacagattac
    14161 cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca gcttgccgta
    14221 gtggggagtc gggcgcattc atggtatggc gagcgatggg gacgattatt ttgcgaaact
    14281 ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga tggtgtggcg
    14341 cataaagcgg ccttacaggt aaatggcgtc agcattgctg tattagggaa tggacttaat
    14401 accattcatc cgcgtcgcca tgcccgactg gctaccagtt tgcttgaaca tggtggggct
    14461 cttgtctcgg aatttcccct cgatgttcct ccccttgctt acaatttccc acgaagaaat
    14521 cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt gcgcagtggt
    14581 tcgctggtga cagcacgttg tgcgcttgag cagggacgtg aagtttttgc cttgccgggt
    14641 ccattaggga atccgggaag cgaagggcct cactggttaa taaaacaagg tgcgattctt
    14701 gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg gttgccagac
    14761 gcccctgaaa attcatttta ttcaccagat caggaagacg tggcattgcc atttcctgag
    14821 ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga acgtgccggc
    14881 caacctgtgc cagaggtagt aactcaacta ctcgaactgg agttagcagg atggatcgca
    14941 gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg tactaatgta
    15001 tttgtttgaa acctatattc acacagaagc tgagttgcgt gtggatcaag acaaacttga
    15061 acaggatctt accgacgcag gatttgatcg agaagatatc tacaatgccc tgctttggct
    15121 ggaaaagctt gcggattatc aggaaggact ggcagaaccg atgcaactgg cctctgatcc
    15181 actctccatg cgtatttata cccctgaaga gtgtgaaagg ctggatgcca gctgtcgtgg
    15241 ttttctgctt ttccttgagc aaattcaggt gcttaacctt gaaacccgtg aaatggtgat
    15301 agagcgagtg ctcgcgctgg ataccgcaga gttcgacctg gaagacctga aatgggtaat
    15361 cctgatggtg ttgttcaata ttccgggctg cgaaaatgcg taccagcaaa tggaagaatt
    15421 actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca gcataagttg
    15481 ttatggcaaa atcagcactg ttcacggtgc gtaataatga gtcctgccca aagtgcgggg
    15541 ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggatgctca cagtatccgg
    15601 cgtgtgacta cgtccgtcct ctgaaatctt cagcggatgg acatatcgtc aaagttctgg
    15661 aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga cgctttggta
    15721 tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc gataaaccgg
    15781 acgaaacagc aattacatgc ccccaatgcc ggacgggcca tctggtccag cgccgctccc
    15841 gttatggcaa aacgtttcac tcctgtgatc gctacccgga gtgtcaattt gccattaact
    15901 tcaaacccat agctggagaa tgccctgagt gtcattatcc gctactcatc gaaaagaaaa
    15961 ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg gtttcggcgg
    16021 aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat agatgttctc
    16081 aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg gtgtgatcct
    16141 gatagcgaaa tagcagtgat gagactgttg gagttaaaac agcgtccggt tgataagggg
    16201 ctgattttaa tcgcagcaaa ttacgagcag cttaaaccct atattgatga caccatgctg
    16261 actgatgcgc agcgtgaaac cattttttcc cgctggccag gtcctgtcac ctttgtcttt
    16321 cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct tgctgtacga
    16381 gtcaccgacc atccgttggt ggttgctttg tgccaggctt atggtaaacc gctggtttct
    16441 accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt tcgcgcacaa
    16501 tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa tccttcagaa
    16561 atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg aaacctatgc
    16621 tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc aatttgctca
    16681 gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg atttcatcaa
    16741 cacactgaac gctttcttta gtgctggtgg taaaggtgcg aatgtgacgg taccttttaa
    16801 agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg ctggtgctgt
    16861 taataccctc aagcggttag aagatggacg cctgctgggt gacaataccg atggtatagg
    16921 tttgttaagc gatctggaac gtctgtcttt tatccgtcct ggtttacgta ttctgctcat
    16981 tggcgctggt ggagcatctc gcggcgtact actgccactc ctttccctgg actgtgcggt
    17041 gacaatcact aatcggacgg tatctcgcgc agaagagttg actaaattgt ttgcgcacac
    17101 tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg atctcattat
    17161 taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat cgctcattca
    17221 tcctggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt ttctggcatg
    17281 gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg tggcacaggc
    17341 ggctcatgcc ttccttcttt ggcatggcgt tctgcctgat gtagaaccgg tcatcaaact
    17401 attgcaacag gagttgtcag cgtgaatcag gccatccagt ttccggatag ggaagagtgg
    17461 gacgagaata aaaaatgtgt atgttttccc gctctcgtga atggtatgca actgacatgc
    17521 gcgatctctg gcgagagtct ggcgtatcgc tttactggag atacgccaga acagtggtta
    17581 gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt aattcaggaa
    17641 caaagtgaag atgatcaagg ctgggtctgg ttactctgat ccagatattc gtccttccat
    17701 ttcacgtaat tattcgcgga atagcgtaac ccagccttct cttcatcact taacgggcgg
    17761 atctgtttga cggggctacc gagatacaga tatccgctct ccagccgttt attttgtggg
    17821 accagactac ccgcaccaat catcacatca tcttctacta ttgcgccatc aagtaaaatt
    17881 gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat caccttgtga
    17941 ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta cgaggattta
    18001 tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat ctgtacataa
    18061 tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc cagacgaacg
    18121 tcaccaatca cgacgctgct atcgtcgatc attacgcgct gaccgatttg tggaaaaaga
    18181 tcgcggtatg ggcgtaaaac atcagacata ctcacctcaa caataaatga tttcctaatg
    18241 actttgggtg cattattggc cttgtgcaag tcttttagta tgcaaaaaag caccgttttg
    18301 tgtgcgaatg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat caaaaaagta
    18361 cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa cgtgaaacac
    18421 ttcacaagat ggtcgtaaca acaaagagaa aaaatcctga aattcagggt tgactctgaa
    18481 agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaagccgcg tcgcaactgc
    18541 tctttaacaa tttatcagac aatctgtgtg ggtactcgaa gatacggatt cttaacgtcg
    18601 caagac
//
LOCUS       DAYPHN010000092        18512 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000092 DAYPHN010000000
VERSION     DAYPHN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.092"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            25..996
                     /gene="yfbL"
                     /locus_tag="ACG0BW_002994"
     CDS             25..996
                     /gene="yfbL"
                     /locus_tag="ACG0BW_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFK7259929.1"
                     /translation="MKKITPLCFISLLLSLPLIIFCQPWVNALPSTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARVTSQDVPIAGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDQLTYTSGADDNASGVAGLLELARLLQQQAPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVRLMIALEMIGYYDSAPGSQDYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVITLLYNSK"
     gene            1099..1602
                     /gene="yfbM"
                     /locus_tag="ACG0BW_002995"
     CDS             1099..1602
                     /gene="yfbM"
                     /locus_tag="ACG0BW_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFK7259930.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHNLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1670..3127)
                     /gene="nuoN"
                     /locus_tag="ACG0BW_002996"
     CDS             complement(1670..3127)
                     /gene="nuoN"
                     /locus_tag="ACG0BW_002996"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFK7259931.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3134..4663)
                     /gene="nuoM"
                     /locus_tag="ACG0BW_002997"
     CDS             complement(3134..4663)
                     /gene="nuoM"
                     /locus_tag="ACG0BW_002997"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFK7259932.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4827..6668)
                     /gene="nuoL"
                     /locus_tag="ACG0BW_002998"
     CDS             complement(4827..6668)
                     /gene="nuoL"
                     /locus_tag="ACG0BW_002998"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFK7259933.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     ITHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6665..6967)
                     /gene="nuoK"
                     /locus_tag="ACG0BW_002999"
     CDS             complement(6665..6967)
                     /gene="nuoK"
                     /locus_tag="ACG0BW_002999"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFK7259934.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6964..7518)
                     /gene="nuoJ"
                     /locus_tag="ACG0BW_003000"
     CDS             complement(6964..7518)
                     /gene="nuoJ"
                     /locus_tag="ACG0BW_003000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFK7259935.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7530..8072)
                     /gene="nuoI"
                     /locus_tag="ACG0BW_003001"
     CDS             complement(7530..8072)
                     /gene="nuoI"
                     /locus_tag="ACG0BW_003001"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFK7259936.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8087..9064)
                     /gene="nuoH"
                     /locus_tag="ACG0BW_003002"
     CDS             complement(8087..9064)
                     /gene="nuoH"
                     /locus_tag="ACG0BW_003002"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFK7259937.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9061..11787)
                     /gene="nuoG"
                     /locus_tag="ACG0BW_003003"
     CDS             complement(9061..11787)
                     /gene="nuoG"
                     /locus_tag="ACG0BW_003003"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFK7259938.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGANVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11840..13177)
                     /gene="nuoF"
                     /locus_tag="ACG0BW_003004"
     CDS             complement(11840..13177)
                     /gene="nuoF"
                     /locus_tag="ACG0BW_003004"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFK7259939.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13174..13674)
                     /gene="nuoE"
                     /locus_tag="ACG0BW_003005"
     CDS             complement(13174..13674)
                     /gene="nuoE"
                     /locus_tag="ACG0BW_003005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFK7259940.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13677..15479)
                     /gene="nuoC"
                     /locus_tag="ACG0BW_003006"
     CDS             complement(13677..15479)
                     /gene="nuoC"
                     /locus_tag="ACG0BW_003006"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFK7259941.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15573..16235)
                     /gene="nuoB"
                     /locus_tag="ACG0BW_003007"
     CDS             complement(15573..16235)
                     /gene="nuoB"
                     /locus_tag="ACG0BW_003007"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFK7259942.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16251..16694)
                     /gene="nuoA"
                     /locus_tag="ACG0BW_003008"
     CDS             complement(16251..16694)
                     /gene="nuoA"
                     /locus_tag="ACG0BW_003008"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFK7259943.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17324..18262)
                     /gene="lrhA"
                     /locus_tag="ACG0BW_003009"
     CDS             complement(17324..18262)
                     /gene="lrhA"
                     /locus_tag="ACG0BW_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFK7259944.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4605 a         5144 c         4783 g         3980 t
ORIGIN      
        1 acgcctttta ttgataattt acatatgaaa aaaataacac ctctttgttt tatatcatta
       61 ctactatcac tccctctgat tattttttgc caaccttggg tcaatgcatt accgtcaacg
      121 ccccgacacg caagtcctga gcaattagaa aaaacagttc gttatcttac acaaactgtg
      181 catccacgta gtgccgacaa tattgataat ctgaataggt ccgcagagta tataaaagag
      241 gtctttgtca gtagcggtgc cagagttacc tcgcaggacg tccccattgc gggtggcccc
      301 tacaaaaaca ttgttgctga ttatggtcct gccgatggac cgctgattat tattggtgcg
      361 cactatgact ctgccagcag ttatgaaaac gatcaattga cctatacgtc gggcgcggat
      421 gataacgcca gtggcgtggc aggattactc gaactggcac gtttgttaca gcagcaagca
      481 ccgaaaactg gcgtgcagct ggtcgcctat gcgtcggaag aaccgccctt ctttcgtagc
      541 gatgaaatgg ggagcgcggt acatgcagct tcgcttgagc gtccagtgag attaatgata
      601 gcgctggaga tgattggcta ttacgactct gcgcctggta gtcaggatta cccttacccg
      661 gcaatgtcct ggctttatcc cgatcgggga gactttattg ccgtggtcgg cagaatgcag
      721 gatatcaacg ccgttcgtca ggtaaaagcg gcattgttgt catcccggga tttatctgtt
      781 tattctatga atgccccagg gtttattccc ggtattgatt tctctgatca ccttaattat
      841 tggcaacacg atattcccgc cgtaatgatt accgacaccg ctttttatcg taataaacaa
      901 taccacttgc ccggtgatac cgcagacaga ttgaattatc agaaaatggc tcaggtagtg
      961 gatggggtta taactttgtt atacaacagt aaataataaa aatttctcga ggcgagtcca
     1021 gaaaaagcga catgtacatt ttattttccc tcgtctgttt taagatgcga aacatgaaat
     1081 tcaacggagg aaaataaaat gggaatgatt ggctattttg cagaaattga ctcagaaaag
     1141 attaaccaac tgcttgagtc tactgaaaaa cccttaatgg acaatattca tgacacgctt
     1201 tcaggattac gccgactgga tattgataaa cgctgggatt ttttacattt tggcctgaca
     1261 ggcacctctg cttttgatcc cgcgaagaat gatccgctga gtcgtgccgt gcttggtgaa
     1321 cacaacctcg aagatggcat agatggcttc ctgggattga cctggaatca ggagttagcc
     1381 gcgacaatcg accgactgga agctctggat cgaagtgagc tgcgtaaaca gttttccatt
     1441 aaacgactca atgaaatgga gatttaccct ggtgtgactt tttcagaaga gttagagggg
     1501 caactctttg cttcgatcat gcttgatatg gaaaagctga ttagtgcgta tcgacgtatg
     1561 ctgcgacaag ggaatcatgc gctgacggtt attgttgggt gatggggtca gggcaccaaa
     1621 aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actgactgct tacatcagcg
     1681 gcatggccaa ccgcacaatg ctaatcagcg gttgcggcca tacacccagc accagtacca
     1741 acagtgcaga gatcagcacc acgataccgc ccgcgctgta ctgccagttt gatggtgcat
     1801 cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt
     1861 agaggccgat tgccgaaccg acaaccacgg cacccaccag ccaccacaag tgtgcctgga
     1921 caccgaccgc cagcacgtag aacttaccaa taaagcccag cgtcatcgga ataccggcca
     1981 gagacagcat catcaccgtc atcaccgccg cgaggatcgg acgatgccag aacagtccgc
     2041 ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc aggctgacca
     2101 cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaacccct accgcttcca
     2161 tcgacatctc gccggtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga
     2221 tagatgagta accgagcaga cgtttgatat tggtctggct cagcgccatc aggttaccga
     2281 agatgataga ggcaaaggcg ataatcgcca gcaccacgcg aatcgcttcg ctgtcaccca
     2341 ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg
     2401 ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga
     2461 acggcaccag agagagtttg aagccgaggc caacaatcat caggccgaaa cctgccagca
     2521 acagcggctc gttgagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt
     2581 cgccagactg cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag
     2641 agagaatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga
     2701 ccaggccaaa cagcggcaaa gagatcagtt cgataccgag gaacagagac gccagatggt
     2761 tggcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcgt
     2821 ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg
     2881 ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agtggcgtaa
     2941 cgtccatagc gcccgcctgg ccgacaaacc agagcgaaac cagcgccgcg ttaagcccaa
     3001 taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa
     3061 ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta
     3121 ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat
     3181 tgccaatcgc ggaatgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca
     3241 gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct
     3301 ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgcaacatc gccagcgaat
     3361 aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga
     3421 agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc atcccaagcg
     3481 ttgccaccgc aaagaacagc gacagtgctg gcagccattt catcttgctc cacagaccgc
     3541 ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccgcag agaataaaca
     3601 gacccgccgc cgacaagccg tgcgcaatca tctggattac cgcgccctgg taggccaact
     3661 ggctgccggt atagatagca atcagcacga agcccatgtg ggaaaccgag gtgtaggcga
     3721 tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa
     3781 cacccagcca catagcgatt ggcgcgaact ctgccgacgc gttcgggaac agcggtaggg
     3841 agaaacgcag cagaccgtaa gcggcggttt tcagcaagat ccccgcgagg tcaacggaac
     3901 cggcggttgg agcctgtgag tgcgcatccg gcagccagcc atgcagcgga accaccggca
     3961 ttttcaccgc aaacgcgatg aagaagccca gcatcagcaa atattccacg ccgttagaca
     4021 ttggcgtatt cagcagctct tcatagttga aggtccagac gccagtcgca ttgtagtgaa
     4081 caaaaaccag cgccaggatg gcgatcaaca tcaccagacc actcgcctgg gtgtaaatga
     4141 agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc ccccacagtg
     4201 cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt
     4261 cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga
     4321 agccctgata tttttcgatc tctttccacg aacagagtac cgccagcaca ccgagcagac
     4381 cggtcagcac gaccatcagc agcgatagcc cgtcaatggc gagatggata gagataccaa
     4441 aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg
     4501 tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg
     4561 tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga
     4621 agccgccgat aaagggaatt aatattagcc agggtagtaa catggcgatc tttattcctt
     4681 gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa ttctcttcgg
     4741 attgggggta gtgtcggatg caccgcgaat gcggcttatc cgacctacgt tcttatatgc
     4801 ccaggggcaa gcccacaatc cttaactcaa cgcagtacca tcaacagtgc cagcaccacg
     4861 accgcaccga tgctcatgga tgccacatac cagcgcagat aaccgttctc gcttaacagc
     4921 agacctttac ctgcaaagcg ggaaaggaca gccgggatgt tcatcattga gttcagcgga
     4981 tcgcgtttca gcaaccaggc aatacccagg aacggcttga cgaacacttt gtcatacagc
     5041 cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca gacggcccgg agcactgttg
     5101 gcgatggagg tcaccagagt acgtttaccc agccacagcc aggctgccag cagaatgccg
     5161 accaccgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg cgccagctcc
     5221 gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa
     5281 aggatcagca gcacaatcag cggcaggctg tgagttatcc ctttcacggc gtgagcgtga
     5341 atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtagag cgaggtcata
     5401 aacgcaccga ccagacctgc caccatcaga ttgatatgac cattcgccat cgcacccgcg
     5461 aggatctcat ccttactgaa gaagcccgca gtgaccagcg gcagtgccga cagtgctgcg
     5521 ccacccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc gcccatcttg
     5581 aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag gaacagcagc
     5641 gctttaaaga atgcgtgagt catcaagtgg aaaatcgccg catcccatgc ctgcacgcca
     5701 agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac acgtttgatg
     5761 tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc aacaatgccc
     5821 accagatgca gaacttccgg cgtcatcagg aacaggccgt gggtacgggc gatcaggtag
     5881 acacccgcgg ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt cgggcctgcc
     5941 atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag atttaccgac cgcaccgccc
     6001 agcagcatca gcgtcgccca catcagcatg ttattgccgt cagcaaagtg cgctggtgcc
     6061 agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat gaacagcgcg
     6121 aaagcgagga acacgtcacc cacacgggtc acgacgaacg ctttcattgc cgctgcacca
     6181 ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc cacgccttcc
     6241 cagccgaggt acatcagcag caggttgtcg gcaagcacca gaactaccat gctggcgatg
     6301 aacaggttgg tgtaagcgaa gaagcgagag tagccctctt caccgcgcat ataccaggag
     6361 gcgtacatgt gaataaggaa acccacacca gtgaccaccg agagcatggt cagcgacagg
     6421 ccgtccagca ccaggttaaa accgatgtta aagtcgccta ccgacatcca cgtccacagc
     6481 ggctggctgt atgcctgctc accgttagcg aagaagtcaa cgccgataaa ggcagttacc
     6541 agcgccgcca ggcccacaga gccaacgccg acgatcgccg agacgttttc agaccagcgc
     6601 ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt taaggcaagc
     6661 atgttcatcc gcgcatctca cttactgaat caatgttcag gttctggcga cgacggtgaa
     6721 gttgcagcag cagcgctagg ccgatactcg cttctgccgc cgcgaggctg atggcgagaa
     6781 tgtacatcac ctgaccgtcg gtctggcccc agtagcttcc ggcgaccaca aaggccagcg
     6841 cggaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg cgacggataa
     6901 ccagaccagt taagccaaga acgaataaga ttgccgcgag gatcagtcca tgttgtaagg
     6961 ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat tgctcagcac
     7021 ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacccg cgagcagcag
     7081 catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac ccactgcttt
     7141 agcactgatt ggcgtaccgt cgataccttg atcgttaaca ccgagaatgg cgtaaacaat
     7201 caccaccagc atgatggccg acaaaatcgc cggaccaatc cacacctgcg gtttcagcca
     7261 ctggcgttcc tgttcaattt ctgaaccgcc caggttgagc atcatcacca cgaacacgaa
     7321 cagcaccata atggcacccg cgtagacgat aatttccagc gcaccggcaa agtaagcgcc
     7381 cagtgagaag aacaccccgg aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg
     7441 taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca ggccacagat
     7501 ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac gtcgataggc
     7561 ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc tgccatccgg
     7621 tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc tttctcgtaa
     7681 accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg aatcgccgtg
     7741 gtcggacagg cttcttcgca cagaccacag aaaatgcagc gtgagaagtt gatgcggaaa
     7801 aattccggat accagcgacc gtctttggtt tctgcttttt gcagcgagat acagccgacc
     7861 gggcaggcta ccgcgcagag gttacaggct acgcaacgct cttcgccgtc cggatcgcgg
     7921 gtcagaacga tacgaccacg ataacggggc ggcagataga ccggctcttc cgggtacatt
     7981 cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg aacctgggtg
     8041 ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat tgcgcctgcc
     8101 agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg cagattttcc
     8161 agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga atcaaaatga
     8221 acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt aacaacgggc
     8281 cttgccagcc accgaagaac agcgtcacca tcagtgcaga gatggtcaca atcccgatgt
     8341 attcgcccac gaagaacaga ccgaacttca tgccggaata ttcaatgtgg taaccatccg
     8401 ccagttcctg ctcggcttcc ggctggtcaa atgggtgacg gtgacacacc gccacgcccg
     8461 cgatggcaaa ggtaataaaa ccaaagaatt gcgggatgac gttccacaca tgcgcctggc
     8521 tgttaacgat gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc atcagggaaa
     8581 gcccgaggaa cacttcgtag ctcagagtct gcgcagaagc acgcatcgcc cccagcaacg
     8641 agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtagaccgcc agaccggcca
     8701 tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaacct gggctgactg
     8761 gcacaatcgc aaaggccagc agcagcgagg taaaggcaat catcggtgcc agggtaaaga
     8821 tgacgcgatc cgagaatttc gggatccagt cttctttaaa gaacatcttg atcatgtccg
     8881 caaccagctg gagcgaaccg ccccagccaa cacggttagg tccgtaacgg ttctggaaca
     8941 gacccagcag gcgacgttcg ccaaagctca tgaatgcccc acaggtgaca accaccagca
     9001 ggatcaccac cgctttgagg atggtcagca ggatctcaat cagttccggt gatatccaac
     9061 tcattgttgt gcctccttga gatcctcaag atgcgcgccc gccagcaccg gagcaatgcc
     9121 ggacataccc atcggcaagc ccacctgccc tgccgtcagc ccttcggcga tttcaaccgg
     9181 cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt gtacccgcgt tcacacccaa
     9241 cttcgcggca tccgccgggt taagtttgat gtacggctgt ggcatacggc tctggaaaac
     9301 cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg tagtacggcg cgatacgcca
     9361 tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca gaccgttttc
     9421 gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc caacttcgtc
     9481 ctggaatttg ttccacgcct gcggggagtt ccagcccgga gcccaggcaa acggcacttg
     9541 cgaacggtgc gcggtcggct ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat
     9601 atcctgcggc tgacgcggct catgaacgct gatattggcg cgcatggcgg tacgaccgct
     9661 gtaacggtgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg catccggcgc
     9721 agcatctttg atacccgcca gttccgggat tttcgccaca acagcgtcaa tcacatggtc
     9781 gagctgcgtc cagtccactt cacggctcag cagggtgctg tgcagcgagt gtaaccaacg
     9841 ccagctttcc agcatgacag ttttgctgtc gtaataagcc ggatcgtaaa cctggaagaa
     9901 acgttgggcg cgaccttcat tattaattac cgtaccgtcg ctttcagcaa agctggcggc
     9961 ggaaagtacc agatgggcgt tttccataat cgctgtgcgt tgatggtcaa ccaccatcac
    10021 cagcggcgct ttagccagcg cagcattcac gcgggtagcg gaagcgtgac gatgcagatc
    10081 gttttccagc accaccaccg catcggcgcg tccggtttcc agttcggtta acgcttcttc
    10141 aagcgatcca ccgcccataa tgcccagccc catgctgttg acggaacggg caatcatggt
    10201 gataccgacg ttagcgccgc gacctttcag ggctttcgcc acgttagccg cagcctgaat
    10261 cacttcagcg ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg cacccgccag
    10321 agcctgcacg atgacgtcga ttttgctttg cagctcaggt tcgataccgt caaccgctgg
    10381 tgcggagtta tccagagcat gggcaatggc aaaacctaaa cgcgcctgat cttcaaccgg
    10441 tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa
    10501 cagcggatgc ttcgctcgtt ggccaatatt gaggattgcc gcaatctgcc agtcagccac
    10561 tttctgtgct gccgccattt cgcgcgcttt acctttcacc gcctgacgca ccgccagtgc
    10621 gacacgagcg ccagtctgag taacgtcttc gcccagcacc agtaccgcat cgtaagattc
    10681 gatttcgcgc agtgccggcg tataaatgcc gccttcacgc agcactttca gcgccagttg
    10741 cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc ccaccagctc
    10801 acgcagcgca aagttgcttt ccacgctggc acgcggagaa ccaataccga tcactttctt
    10861 cgactgacgc agaatatctg ctgcgccctg cattgcctgt tcggcgttga gggtaatgaa
    10921 atcatcgcca cgacgctgta ctggctgacg cggacgatct ttcaggttga cgtaaccgta
    10981 accgaaacga ccacggtcgc agaggaagta gtggtttact gtaccgttgt aacggttttc
    11041 gattcgacgc agttcgccat aacgctcgcc ggggctgatg ttacagccga tggaacattg
    11101 ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct cggagtgcgt
    11161 tttgtcggtg aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc
    11221 cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt aaacgcccag
    11281 atccgtgccg tcagcgtaat ctttgtagta acgcacgcag cggtagcagg cgatgcagcg
    11341 gttcatttcg tgagagatga atggccccaa atcctgatta cggtgggtac gtttggtgaa
    11401 acggtagcga cggaagctgt gtccggtcat caccgtcata tcctgaagat ggcagttacc
    11461 gccctcttca cataccggac agtcgtgcgg gtggttggtc attaaccact cgaccacgct
    11521 ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat cggaagccgg
    11581 tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac
    11641 cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat
    11701 atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga cctcgtattc
    11761 tttgccgtct acatgaattg tagccattag catgcttcca gttttctcag tcagagactg
    11821 agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga atcccattaa
    11881 tcaaatgggt attgctgaac ggctgtttga ttcccgcctc aaattcttcg cggaaatatt
    11941 tgatggcgct ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa gttttacccg
    12001 ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc gcccggttga ccttcgccac
    12061 gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc
    12121 agccgcagga ctcacgggcg aaaaactctt ccaggttacg caccagcgac accatgttga
    12181 tctcatggtc aaccgccatc gccagcgccg tacccagacg gcttcccgct ttaccgatac
    12241 tttcgaattc catcggcagg tcaaggtgcg cttcagtcaa gaagtcagta cccgcgccgc
    12301 ctggctgcca ggctttgaat ttcagaccgt cacgcatacc accggcgtaa tcttcgagga
    12361 tctcgcgtgc ggtggtgcca aacggcagtt cccacaggcc cggatttttc acccgaccgg
    12421 agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga taccactcca
    12481 cgccgttagc aaggatcgcc ggaacgttac acagggtttc gacgttattg acacaggtcg
    12541 gtttgcccca tacgccggag gttgccggga aggggggttt cgagcgtggg ttagcacgac
    12601 gcccttccag ggagttgatt aacgctgttt cttccccgca gatgtagcgc cctgcaccgg
    12661 tatggacgaa cagttcgaag tcgaaacctg ttcccataat gtttttgcca agcaagcccg
    12721 cttcagtcgc ttcggcaatg gcgcggcgca gattcactgc cgcttcaata tattcgccac
    12781 gcaggaagat gtagccacgg taagctttca gcgcaaacgc ggagataagc ataccttcca
    12841 ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt ataggtgccc ggttccattt
    12901 catcggcgtt acacagcagg taacggatgt tcatggattc gtctttcggc atcaggctcc
    12961 acttcaggcc agtcgaaaag cccgcgccgc cgcgcccttt cagaccagcg tcttttacct
    13021 gattaacgat ttcgtccgga gacagcccgg tcagcgcctt acgcgcgcct tcgtaaccgt
    13081 ttttgctgcg atattcgtcc agccacactg gctgtttgtc atcgcgcaga cgccaggtca
    13141 gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca gcagttcagg
    13201 gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg atcatcatgt ttggcccttt
    13261 atcacagttc cccaggcagc aagttggcag cagcgtaaag cggccatcaa atgtcgtttg
    13321 ccctggtttg atgttcagct ttttctcgag cgccgcctga ataccctgat aaccgttgat
    13381 atgacagacc acgctgtcgc aataacggat cacatggcgg ccaaccggct ggcggaagat
    13441 ctggctgtag aacgttgcca cgccttcgac gtcgctcgcc ggaataccca gcacatcggc
    13501 gatcgcgtgg atcgcgccat ccggcaccca gccacgctgc ttctgaacga ttttcagcgc
    13561 ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg
    13621 ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt gcataattag
    13681 cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag gtcagacacc
    13741 aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg cgtacggata
    13801 cgggtgcgat aactcatggt gctaccgtcg ctggtcaggt agtaactgtt gatccccttg
    13861 gtcgcctcaa tcatctggaa agattcattg gctggcatca ccggacccca cgacacttgc
    13921 aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg
    13981 gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca ctgctcaaga
    14041 atgcgcagac tctggcgcag ctcttccact ttcagcatta cgcgggtgta gcagtcagaa
    14101 acaccaccac ccaccgggat ttcaaagtcg aagttttcat agccagaata aggacgcgcc
    14161 ttacgcacgt cgaagtcgat cccggtggcg cgcaggcccg cgccagtggt gccccactcc
    14221 agcgcctctt tcgcgccata ggcggcaacg ccctgggaac gacctttcag aatggtgttt
    14281 tgcagcgccg ctttctcgta agacgccaga cgtttcggca tccagtcgag gaactcacgc
    14341 agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc
    14401 gggtgcatac gaaaaccagt gattgcttcc accagatcgt aaattttctg acgatcggta
    14461 aaggcgaaga acactggcgt catcgcgccg acgtcctgaa taaaggtgga tatatacagc
    14521 agatggctgt tgatgcggaa cagctcagaa agcattacgc gaatgacgtt aacgcgatcc
    14581 ggcacggtga tcccggccag tttctctacc gccagcacgt aaggcatttc gttaacgcag
    14641 ccgccgaggt attcgatacg gtcggtatac ggaatgtagc tgtgccagga ctggcgttcg
    14701 cccattttct ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc gacaatttct
    14761 tcgccatcaa gctgcaaaac gatgcggaaa gccccgtgcg ccgacgggtg gttcgggccg
    14821 aggttgagga acatgaagtc ctcgttttcg gtgccgcgct tcatccccca ctcttccggt
    14881 ttgaaggtca gggcttccat ctccaggtcc tgtttggctt tggtcagctc aaacggcgag
    14941 aattcggtag cgcgcgccgg ataatcttta cgcagcgggt gacctttcca ggtttgcggc
    15001 atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa tgccaaacag atcccaggtt
    15061 tcacgctcat accagttggc gttcgggaac agtttggtga aggtcggtac gtgcaggtcg
    15121 ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga aatcagatgg
    15181 tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc
    15241 atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa gaaatcgcca
    15301 acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg agtcgcctga
    15361 acagtaaagg catccggccc aaaacggttg cgcagttcgc caatcaccgg atcatcaaga
    15421 tgatcgcggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt gttcaccatt
    15481 gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt taggaataag
    15541 cgaagaaaaa tccctttgcc gacaggcgca aattaaatct cgtcaggtgt acgcaggttg
    15601 gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa
    15661 acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttgccgat agattcctgc
    15721 aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg gatatacaca
    15781 tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta cataccgccg
    15841 gagttggcac aggcacccat tgagataacc cattttggtt ccaacatctg gtcatacaga
    15901 cgctgaataa ccggtgccat tttggtaaag caggttcctg ccaccaccat caggtcagcc
    15961 tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta
    16021 aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg ccaaattgag
    16081 tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt tttgttaact
    16141 tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg gtcgttctca
    16201 ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga
    16261 cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc
    16321 cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac aaaaataaaa
    16381 attgcagctt ccacaaagcc tacccagccg ctttcgcgga tagaggttga ccatgcgaac
    16441 agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata aaacttggcg
    16501 gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg aacgtttttc
    16561 gaccttgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag gcaacacagg
    16621 ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac ttcagtggat
    16681 gttgacatac tcattgctta ctcatcaaaa gtagcgccag attcactgct cttcacggca
    16741 gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaatc actgtagaaa
    16801 ttcttttata cagctaattg atgtggtctt ttactccttt ctataacctt ttgtcaactt
    16861 taacaaaagt ttcttcacat tagtttacat aatatcaaca ccattagcat ttaatgccct
    16921 ttcaccccag atccttgacg actccaggat aattagatgt tgttgaatcg tgtccgttgt
    16981 gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt cctcccccca
    17041 aaagagagta ttttcttgat ctgtgacacg cttttgtcat tccataacaa aaacgcagca
    17101 acaaatttac gtatttttta acatcattgt agcaggtgat ttttttcagg cgattatttg
    17161 tgcgttcggg acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcacg
    17221 atacgaggaa ggctttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct
    17281 cgtttttaca ctattgtctc agaaattatc tatggtccgt cgattactcg atatcccttt
    17341 caatcaacaa tgagtcatcg ccttccggag cagacattgg gctgtattgc cacggattat
    17401 ggtagctctc catcgcctga tagatcacct gagccagctc attattactg gacgggtcat
    17461 agcacagcaa atattcggta tcgggtagcg gcggtaagcc atctactcca ctcagcacac
    17521 gcaggtctgg actcatcatc tcgaccggcc ttgccgtcac gccaagaccg gcttttactg
    17581 ccgcacgaac tgccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt
    17641 tattaagcgt cgccagcacc atatcgcgaa acgggctagg atcatccagc aatacaagag
    17701 ggatcggctc gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg
    17761 tacgcaggtt caatgcctta aacgttgaag gacgatgggt ggttaccatc aaatccactt
    17821 cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta
    17881 gtttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt
    17941 catctgaagc accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg
    18001 cctcatcatt aaaacgcaga atttttctgg cgtaaccaag aagttgaatg ccatgttcag
    18061 ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacaagt tgttccagac
    18121 gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa
    18181 aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta
    18241 tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact gagctggtta
    18301 atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc
    18361 cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt
    18421 tccgttataa tgcctgaagt agatcacaga atatatcttc aggaatcgca tatctattaa
    18481 gttactcact cttttgtatt tatgacatgc gc
//
LOCUS       DAYPHN010000093        18389 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000093 DAYPHN010000000
VERSION     DAYPHN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.093"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(19..95)
                     /locus_tag="ACG0BW_003010"
     tRNA            complement(19..95)
                     /locus_tag="ACG0BW_003010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:Arg,seq:acg)
     gene            complement(293..369)
                     /locus_tag="ACG0BW_003011"
     tRNA            complement(293..369)
                     /locus_tag="ACG0BW_003011"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Arg,seq:acg)
     gene            complement(568..644)
                     /locus_tag="ACG0BW_003012"
     tRNA            complement(568..644)
                     /locus_tag="ACG0BW_003012"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Arg,seq:acg)
     gene            complement(648..740)
                     /locus_tag="ACG0BW_003013"
     tRNA            complement(648..740)
                     /locus_tag="ACG0BW_003013"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:Ser,seq:gct)
     gene            complement(1056..1241)
                     /gene="csrA"
                     /locus_tag="ACG0BW_003014"
     CDS             complement(1056..1241)
                     /gene="csrA"
                     /locus_tag="ACG0BW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFK7259945.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1476..4106)
                     /gene="alaS"
                     /locus_tag="ACG0BW_003015"
     CDS             complement(1476..4106)
                     /gene="alaS"
                     /locus_tag="ACG0BW_003015"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFK7259946.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGTNFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4234..4734)
                     /gene="recX"
                     /locus_tag="ACG0BW_003016"
     CDS             complement(4234..4734)
                     /gene="recX"
                     /locus_tag="ACG0BW_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFK7259947.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4803..5864)
                     /gene="recA"
                     /locus_tag="ACG0BW_003017"
     CDS             complement(4803..5864)
                     /gene="recA"
                     /locus_tag="ACG0BW_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFK7259948.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5944..6441)
                     /gene="pncC"
                     /locus_tag="ACG0BW_003018"
     CDS             complement(5944..6441)
                     /gene="pncC"
                     /locus_tag="ACG0BW_003018"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFK7259949.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6586..7671)
                     /gene="mltB"
                     /locus_tag="ACG0BW_003019"
     CDS             complement(6586..7671)
                     /gene="mltB"
                     /locus_tag="ACG0BW_003019"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFK7259950.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7927..8490
                     /gene="srlA"
                     /locus_tag="ACG0BW_003020"
                     /gene_synonym="gutA"
     CDS             7927..8490
                     /gene="srlA"
                     /locus_tag="ACG0BW_003020"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFK7259951.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8487..9446
                     /gene="srlE"
                     /locus_tag="ACG0BW_003021"
                     /gene_synonym="gutE"
     CDS             8487..9446
                     /gene="srlE"
                     /locus_tag="ACG0BW_003021"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFK7259952.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9457..9828
                     /gene="srlB"
                     /locus_tag="ACG0BW_003022"
                     /gene_synonym="gutB"
     CDS             9457..9828
                     /gene="srlB"
                     /locus_tag="ACG0BW_003022"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFK7259953.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9832..10611
                     /gene="srlD"
                     /locus_tag="ACG0BW_003023"
     CDS             9832..10611
                     /gene="srlD"
                     /locus_tag="ACG0BW_003023"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFK7259954.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10717..11076
                     /gene="gutM"
                     /locus_tag="ACG0BW_003024"
     CDS             10717..11076
                     /gene="gutM"
                     /locus_tag="ACG0BW_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFK7259955.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVVIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11143..11916
                     /gene="srlR"
                     /locus_tag="ACG0BW_003025"
                     /gene_synonym="gutR"
     CDS             11143..11916
                     /gene="srlR"
                     /locus_tag="ACG0BW_003025"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFK7259956.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            11909..12874
                     /gene="gutQ"
                     /locus_tag="ACG0BW_003026"
     CDS             11909..12874
                     /gene="gutQ"
                     /locus_tag="ACG0BW_003026"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFK7259957.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12871..14385)
                     /gene="norR"
                     /locus_tag="ACG0BW_003027"
     CDS             complement(12871..14385)
                     /gene="norR"
                     /locus_tag="ACG0BW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFK7259958.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPDVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14572..16011
                     /gene="norV"
                     /locus_tag="ACG0BW_003028"
     CDS             14572..16011
                     /gene="norV"
                     /locus_tag="ACG0BW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFK7259959.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLGIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            16008..17141
                     /gene="norW"
                     /locus_tag="ACG0BW_003029"
     CDS             16008..17141
                     /gene="norW"
                     /locus_tag="ACG0BW_003029"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFK7259960.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWIMDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCTE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17219..>18389)
                     /locus_tag="ACG0BW_003030"
     CDS             complement(17219..>18389)
                     /locus_tag="ACG0BW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546942.1"
                     /note="carbamoyltransferase; involved in the formation of
                     the cyanate group of hydrogenase; catalyzes the formation
                     of carbamoyladenylate from carbamoylphosphate and then
                     transfers the carbamoyl group to HypE; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFK7259961.1"
                     /translation="RMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADL
                     KNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVS
                     SQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGENGALWGGEC
                     LRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNW
                     SVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCDGVT
                     HPVTMPQVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITT
                     LVFSGGVTHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARILHAA"
BASE COUNT         4314 a         5111 c         4669 g         4295 t
ORIGIN      
        1 tgaagatttc ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
       61 tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaatttatt ttactgctga
      121 tattgatacc gctactaaag ccatatcaag taagagatgg tgcatccggg aggattactc
      181 gccttgcggc tcgcccttcg ggccgttgct aaagcaacgt tatcctccct ggtgcctgcg
      241 gttattaccg ccacctcttc cataccttca tcgctaaagg ttacggagaa tatggtgcat
      301 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
      361 tacggatgca tcgggaaact tactttactg cagatttttt gataccgcta ctaaagccgt
      421 atcaagtaag agatggtgca tccgggagga ttactcgcct tgcggctcgc ccttcgggcc
      481 gttgctaaag caacgttatc ctccctggtg cctgcggtta ttaccgccac ctcttctata
      541 ccttcatcgc tgaaagtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg
      601 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
      661 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
      721 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
      781 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
      841 ccacactttt atcgtttgca cacttcacgc tcaattagtc tgtaaaaacg tcaaaaagag
      901 tgttttatca acaaaaaaac ggaggtctgt cagatagcag taatgcaaaa aaatggagac
      961 ttaagttgaa tgaacgggag taaagcgaaa agacaatgga gtgaagaaga aattttgagg
     1021 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
     1081 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
     1141 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
     1201 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
     1261 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
     1321 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
     1381 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
     1441 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
     1501 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
     1561 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
     1621 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
     1681 tcgactaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
     1741 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
     1801 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
     1861 agctgttgta actctttttc cagctgacgc gtacgttcca gtactgagcg cactttatca
     1921 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
     1981 gcatgaacgg tggcgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
     2041 gcagtacccg attcagagat gatgcggaac agaccaatat caccggtgcg gcttgcgtga
     2101 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac gcgctcatca
     2161 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
     2221 ttggtttcga tcggcaagtt acggcgaatc tgtgcgttca ccaggtcttc gaccgcacga
     2281 atctcttctg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac tttgtcgtta
     2341 accagtgaac ctttctgcga tacatgagta cccagaacct ggcgcagcgc agcgtgcatc
     2401 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcttcatc aacatcagcc
     2461 tgcaccgcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
     2521 tggccgtatt tctgcgtatc ttccaccgca aaggagaagt tagtgccttt cagttcgcct
     2581 ttatcaccaa cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca
     2641 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact
     2701 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactgtc aacacggatc
     2761 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc
     2821 attgctgctt cgaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
     2881 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc
     2941 gtatcaccag aaagttttgc cagctcttca tccagcagcg ccagaccgcg ctccagagta
     3001 cgggcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
     3061 aggtcttcac ccgcagagcc cattacgtcg atcagcggac caaccagttt gtagaagaag
     3121 gtttctttcg cgcccagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt
     3181 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
     3241 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact
     3301 ttcgctaccg cctggatcag cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
     3361 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
     3421 ggttccatcg tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca
     3481 atgtagcgat cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg
     3541 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
     3601 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
     3661 atccctactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
     3721 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
     3781 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
     3841 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
     3901 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
     3961 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
     4021 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
     4081 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
     4141 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
     4201 tttcttgact gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
     4261 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
     4321 tgaaaagaca gttggcaaag gttcgccata ttttcgcgtc gcctgatcgc gcgccagtgc
     4381 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
     4441 ctgattcagt tcctgacgaa tacgcgcagg tccataacct ttgcggctac ggctggcgat
     4501 aaagcgcgcc acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
     4561 gcgctcgtaa tcttctgccg tggcatcaat ctcttccggg ccatttttgc ccataatcgg
     4621 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
     4681 cgcacgatcc aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata
     4741 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccttgtgtat caaacaagac
     4801 gattaaaaat cttcgttagt ttctgctacg ccttcgctgt catctacaga gaaatccggc
     4861 gttgagttcg ggttgctcag cagcaactca cgtactttct tctcgatctc tttcgcggtt
     4921 tccgggttat ctttcagcca ggcagtcgca ttcgctttac cctgaccgat cttctcacct
     4981 ttgtagctgt accacgcacc tgctttctcg atcagcttct ctttcacgcc cagatcaacc
     5041 agttcgccat agaagttgat accttcgccg tagaggatct ggaattcagc ctgtttaaac
     5101 ggcgcagcga ttttgttctt caccactttc acacgggttt cgctacccac cacgttttcg
     5161 ccctctttca ccgcgccgat acgacggatg tcgagacgaa cagaggcgta gaatttcagc
     5221 gcgttaccac cggtagtggt ttccgggtta ccgaacatca caccaatttt catacggatc
     5281 tggttgatga agatcagcag cgtgttggac tgcttcaggt tacccgccag cttacgcatc
     5341 gcctggctca tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc gccttcgatt
     5401 tccgctttcg gcgtcagtgc cgccacggag tcaacgacga taacgtctac tgcgccagaa
     5461 cgcgccaggg cgtcacagat ttccagtgcc tgctcgccag tgtccggctg ggagcacagc
     5521 aggttgtcga tatcgacgcc cagtttacgt gcatagattg ggtccagcgc gtgttcagca
     5581 tcgataaacg cacaggtttt accttcacgc tgcgctgcgg cgatcacctg caacgtcagc
     5641 gtggttttac cggaagattc cggtccgtag atttcgacga tacggcccat cggcagacca
     5701 cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag agatggtttc cacatccatg
     5761 gaacggtctt cacccaggcg catgatggag cctttaccaa attgtttctc aatctggccc
     5821 agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg
     5881 ccgggtaata ccggatagtc aatatgttct gttgaagcaa ttatactgta tgctcataca
     5941 gtatcaagtg ttttgtagaa attgttgcca caaggtctgc aatgcatacg cagtagcctg
     6001 acgacgtacc gcatcacggt caccgctgaa gcattcccgc cgggtaatgc cttcaccacg
     6061 ggcagtggca aaagcaaacc agacggtgcc gacaggcttc tcttcactgc cgccatccgg
     6121 cccggcgata ccactaatag acacagcata atcagcacga gccgctttca gtgcgcctat
     6181 cgccatttcc accacgacag gctcgctcac cgcgccatgc tgcgccagcg tctcttcacg
     6241 tacgccgatc atttgcgctt tggcttcgtt actgtaggtg acaaatccgc gttcaaacca
     6301 ggcggaactg ccggcaatat cggtaatcac tttcgctacc caaccgccgg tacaagactc
     6361 agcagttgtt acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt cgcttaactg
     6421 catcagttca ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc cggacaagat
     6481 agcactttcg tcaggaagat ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa
     6541 agctgattag ccagagggaa gctcacgctc ccctcttgca aatagttact gcacgcgcgc
     6601 cagcgccacg gcttgtccta actgccagac cgccattgcg taatgggtac tgtggttgta
     6661 acgggtgatg gtgtagaagt tcggcagacc gtaccagtac tggtaaccgg taccgacatc
     6721 cagacgcagc aggctggctt gttgatggtt gcccaacggc tgctgtggcg ttaaacctgc
     6781 cgcggcaagc tgcgaaatgc tgtacctggt tttgaagcca tttggcaagc ctggagcctg
     6841 accgtttgcc atcaccgcaa cctgatcacc tttcacccag ccgtgcgctt tgaaatagtt
     6901 cgccacgcta ccgatcgcat caaccggatc ccacaggttg atatgcccgt cgccgctgaa
     6961 atctaccgca tattgtttgt aagacgacgg cataaactgt ccgtagccca tcgccccggc
     7021 aaaggaacct ttcagattga gcggatcgtc ctgctcatcg cgcgccatca gcaggaaagt
     7081 ttccagctcg ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag
     7141 cgcatcgagg atacgggttt tccccatcac gcgcccccag cgggtttcaa cgccgataat
     7201 cccgacgata atttccggcg gtacaccata cacctgccac gcgcgattca acgcatcttc
     7261 atactgattc cagaaaacca caccgttctg cacgttgtcc ggcgtaataa attttttgcg
     7321 ataacgcagc catgcgccgt tcggacctga tggcggtttc accgatgtgg ttggtgcctg
     7381 gttatccatc agccgcagta ccgaatccag acgtttcgcc tgggagagaa tttcctgcaa
     7441 ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg atgaactgct gggcattcgg
     7501 gttattagcg aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc
     7561 gccagacggc gttccggtgg tcgtttcagt ctcagtaggt tttggcttgc tgctacaggc
     7621 ggcaagcaac acaaaaaggg gaagcaatgt tacataacga cgcttgaaca tgaggggtcc
     7681 atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc acgccccgca caaggaagcg
     7741 gtagtcactg cccgatacgg actttacata actcaactca ttctcctcgc tatcctttta
     7801 ttgaaacttt caagttaaaa tatttatctt tcattttgcg atcaaaataa cacttttaaa
     7861 tctttcaatc tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag
     7921 agaacaatga tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag
     7981 ggcggagagg tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg
     8041 gttatcatga acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa
     8101 cgttgcgccg gtaaccctgt ttcccgttac ctgctgctac cgtgcattgg cacgtttgtc
     8161 ttttgcaatc cgatgacctt aagcctgggt cgctttatgc cggaaaagta caaacccagc
     8221 tactacgcgg ccgcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac
     8281 ccaggcgaac tgtttgttta tcttggcatt gccagcggtc tgacaacact gaacctgcca
     8341 cttggcccac tggcggtgag ttatctgctg gttggtctgg tcaccaactt cttccgcggc
     8401 tgggtgaccg atctgaccac cgccattttt gagaaaaaga tgggcattca acttgaacaa
     8461 aaagttcatc tggcaggagc aacatcatga cgcgtattcg gatcgaaaaa ggaacgggtg
     8521 gctggggcgg cccgcttgag ctggaagcca cgccgggcaa aaaaatcgtc tatatcaccg
     8581 ccggtacccg gcctgcgatt gtcgacaaac tggcacagct tactggctgg caggctattg
     8641 acggatttaa agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta atcgactgtg
     8701 gcggcacatt acgctgcggc atctatccga aacgacgtat tcccaccatt aatatccact
     8761 cgacgggcaa gtccggtcca ctggcgcagt acattgtaga agatatttat gtctctggcg
     8821 taaaagaaga aaacatcact gtagtgggtg atgcgacacc acaaccctct tccgtgggcc
     8881 gtgactatga caccagcaag aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg
     8941 gaatgggtat gggttctgcc gttgccgtgt tgtttcaatc tggtcgtgac accatcgaca
     9001 ctgtattaaa aaccattctg ccgtttatgg cgttcgtctc ggcgcttatc ggcatcatta
     9061 tggcttccgg tcttggcgac tggattgccc acggtcttgc tccgctggct agccatcctc
     9121 tggggctggt catgctggcg ctcatctgct ccttcccgct gctttcacct ttcctcggcc
     9181 caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt ggtctcggca
     9241 atattccgcc tcatctggct ttacctgcac tgtttgccat caacgcgcag gcggcctgcg
     9301 acttcatccc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg
     9361 tcccttctgt actggtgagc cgctttttaa ccggcgcgcc gactgtactg atcgcctggt
     9421 ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta tcagaccacc
     9481 atcacccgta tcggcgcgag tgccactgac gccctcagcg accagatgct catcaccttt
     9541 cgtgaaggcg cgcctgcgga tctcgaagag tattgcttca ttcattgcca cggcgagttg
     9601 aaaggtgcac tccatcccgg tttgcaattt tcgctcgggc agcatcgcta tccggtgacc
     9661 gccgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc
     9721 gatggtttaa gcgaagcgga atttccgggc actgtccatg tggcaggccc tgtacccgac
     9781 gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa aatgaatcag
     9841 gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct
     9901 gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagctgc aaatgtggca
     9961 caagaaatta acgccgaata tggtgaagga acagcgtacg gttttggtgc tgacgccacc
    10021 agcgagcaaa gcgttctggc gctttctcgt ggggtagatg aaatctttgg tcgcgtggat
    10081 ttgctggtct acagcgccgg aatagcaaaa gcagccttta tcagcgattt ccagcttgga
    10141 gattttgacc gctcgctaca ggtgaatctg gtgggctatt tcctgtgtgc ccgtgaattt
    10201 tcgcgtttga tgatccgcga cggcattcag ggacgcatta ttcagatcaa ctcgaaatcc
    10261 ggcaaagtgg gcagcaaaca caactctggt tatagcgccg cgaaatttgg tggcgtcggg
    10321 ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca ttcactgatg
    10381 ctcggtaatc tgctgaaatc gccgatgttt cagtcgctat tgccacaata tgcgaccaag
    10441 ctgggtatca aaccggatca agtcgagcag tattacatcg acaaagtacc gctcaaacgc
    10501 ggctgcgatt atcaggatgt gctgaatatg ctgctgttct acgccagtcc taaggcgtcg
    10561 tactgcactg gtcagtcgat caatgtcacc ggcggtcagg tgatgttctg attaacagcg
    10621 gagatccgtt aaggatctcc gcgagactaa taaaatgcct gatgcgctac gctcatcagg
    10681 catacaggac ttccgccact acattaagga aaagttatgg tatccgcact catcaccgtc
    10741 gccgttatcg cctggtgtgc gcaactggcc ttaggcggtt ggcaaatttc tcgttttaac
    10801 cgtgccttcg acacactatg ccagcaaggg cgggttggcg tgggccgttc cagcgggcgc
    10861 tttaaaccgc gggtcgtggt cgtcatcgcc ctggacgatc agcaacgcat cgtcgacacc
    10921 ttgtttatga aaggactgac cgtcttcgcc cgaccacaaa aaattcccgc aattaccggt
    10981 atgtatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat
    11041 gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt
    11101 gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt
    11161 caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga agaattggcg
    11221 caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc
    11281 ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc cgatccgcct
    11341 atcgatcata aaacactcat caacacccac aagaaagagc tgattgcaga agctgccgtt
    11401 agttttatcc atgatggcga ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg
    11461 gttcccctgc tctcgcgctt taataacatc acagtgatga ccaacagcct gcatatcgtc
    11521 aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg aacatttcgt
    11581 aaaaaatcgg cctcatttca cgggcaactg gcagagaatg ccttcgagca tttcaccttc
    11641 gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac cacgttcaac
    11701 gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg
    11761 gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga aagcgtcgat
    11821 aagctaatta ccgacgcagg tatcgatcca gcgtttcgtc aggcgctgga aaagaaaggg
    11881 atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg cgggacgtca
    11941 gacgttaatg ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt
    12001 tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg
    12061 caaatcgggc cacattggta agaaaatcgc cgcaacactt gccagtaccg gcacaccggc
    12121 tttttttgtc catccggcag aggcattgca cggcgacctg gggatgatcg aaagccgcga
    12181 tgtgatgctg tttatctctt actccggtgg cgcgaaggaa ctggatctga ttattccgcg
    12241 tctggaagat aaatctatcg cgctgctggc gatgaccggc aaaccgacgt caccgctggg
    12301 cctggcggcg aaagcggtgc tggatatctc cgtagaacgc gaagcctgtc cgatgcacct
    12361 tgcgccgacc tccagcaccg tgaataccct gatgatgggt gacgcgctgg cgatggcggt
    12421 catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccacccag ccggggcact
    12481 gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgatgacg ctatcccgca
    12541 ggtagcgtta accgccagcg tgatggatgc gatgctggaa ctcagccgca ctggtctggg
    12601 gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg acggcgattt
    12661 acgtcgctgg ctggttggcg gcggcgcact caccacgcca gttaatgaag cgatgacgac
    12721 aggcggcacc acattacagg cacaaagtcg cgccatcgac gccaaagaga tcctgatgaa
    12781 gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg gcgcaattaa
    12841 cttgcaggat ttctatcagg ccgggattat ttaatccttc aatcccagac gtttcgccag
    12901 ccgatgcagg ttggcgacgt cagtttccag cattcgcgcg caggcagccc agttgtgatg
    12961 attttgtgcc agtgcctggc gaatggtttc acgctggaac gcttctgtcg cttcacgaag
    13021 attttgctta acaacgggca ccgccgctgc ttctggcggc ggcaacgtca catcaggaaa
    13081 agcaaaatgt tgcgcctcaa gaatcacttc atcgccgctg cgggttgctc tcgccagcac
    13141 taccgcccga tgaatagcat gttccagttc acgaacgttc cctggaaaat tgtagtgttg
    13201 cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc gcaaacgaca
    13261 ctgctcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg
    13321 cactgaaagt ggaaacacgc tcaggcgatg aaacaaatcg gcgcgaaatc gccctgccag
    13381 tacctcttcc cgtaagtcac ggttagtcgc cgccagcacg cgcacatcga cccgcaaact
    13441 gcggtcatcg ccaacacgct gaatatcgcc atactgcaac actcgcagca acttggcctg
    13501 caatgccaac gacaactcgc cgatctcatc gagaaacagt gtgccgttat ccgccatttc
    13561 aaacttcccg ctgcggttac tgatagcgcc agtaaacgcc cctttcacat gcccgaacag
    13621 ttcactttcc gccacacttt ccggcagtgc ggcacagttg agatagacca gcggattcac
    13681 cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc
    13741 accgctgatc aggacgttga gatcggacgc cgccacaatc tcaatctctt ttttcaattg
    13801 cgtcatgcct ggcgacaagc caatcatctg cgtctgtttc accgcttcaa acggcgcggc
    13861 atcgcccggc agcatattct ggctttccag ttgctcaatc agcaacgcat tgcttaacgc
    13921 tcccgccgcc agcgcggcaa tcaggcgtaa ctcttcgtcg ctgaaaacat cgaactgatc
    13981 gggctgcatc ccgtcgagcg tcaatgcgcc gatcaggttt tgcccggcaa acaatggcag
    14041 accaacgcag gcgtgaacct tcagactctc ctgcccggga atcaaaccgt catagggatc
    14101 gggcaattcg ctgtctgcgg gaaagcgcac cacgtccccg gcgcgggcaa tcgcttccag
    14161 ccgtggatgc ccttccagcg caaagcgtct accgagtaca tcctttgcca gcccgtcgat
    14221 ggcaagcgga ataaactgcc gcgagtcgta acgcagcaat gcagacgcat cgcactccag
    14281 cacctgacgc agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg
    14341 caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc
    14401 aatttgacta tagatattgt catatcgacc atttgattaa tagtcatttt gactactcat
    14461 taatgagcat gaattaattt acacgtaaaa atcaatcaga taaaaaactg gcacgcaatc
    14521 tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt
    14581 gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg tgattttcac
    14641 ggcacagaat ataaaacgct gcgcggcagc agctacaaca gctacctcat tcgcgaagaa
    14701 aaaaacgtac tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg
    14761 cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc agaagaggac
    14821 cacgccgggg cgctgaccga actgatggca caaattcccg atacgccgat ctactgtaca
    14881 gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg
    14941 gtgaaaactg gcgacacgct gggtatcggc aacggcaaac agctcatttt tgtcgaaaca
    15001 ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc ggtgctgttc
    15061 agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat
    15121 cagacggagc tttttgagca gtgccagcgt tactacgcca atatccttac gccgttcagc
    15181 cgcctggtaa cgccgaaaat taccgagatc ctgggcttta acttaccagt cgatatgata
    15241 gcgacctctc acggcgtggt atggcgcgat aacccaacgc aaattgtcga gctgtacctg
    15301 aaatgggcgg ctgattatca ggaagacaga atcaccattt tctacgacac catgtcgaat
    15361 aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga cccacgcgtg
    15421 gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc
    15481 cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc
    15541 gccgggctgg tggaggagat gaccggatta cgctttcgta acaaacgcgc cagtgctttc
    15601 ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg
    15661 ggtttcgaaa tgtcgcttag cctgaaagcc aaatggcgac cggaccagga cgctctggag
    15721 ctatgtcgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag
    15781 agcacggtga atacggtggt taaagaagaa acctctgcca ccacgacggc tgacctcggc
    15841 ccacggatgc agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg
    15901 caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct gtgcccggaa
    15961 tgctcccttg gcaaagacgt ctttgacgaa ctggcatcgg aggcaaaatg agcaacggca
    16021 ttgtgatcat tggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg
    16081 acgcctgtat tccattaacc ctgattgccg ccgacagcat ggatgagtac aacaaaccgg
    16141 acctcagcca tgttatcagc cagaggcaac gtgccgatga ccttacccgc cagacggcgg
    16201 gtgaatttgc cgagcagttt aatctgcacc tgtttccgca cacctggata atggatatcg
    16261 atgccgaagc ccatgtggtg aaaagccaga ataatcagtg gcaatacgac aagctggtgc
    16321 tggcaactgg tgccagtgcc tttgtcccgc cagtgcctgg gcgtgagtta atgctgacgt
    16381 taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt
    16441 tgattgttgg cggtggcttg attggtagcg aactggcgat ggatttttgt cgtgcaggca
    16501 aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcgttaatg ccaccggaag
    16561 taagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgctg ttgaaatctc
    16621 agttgcaggg gctggaaaaa acggattctg gcattctggc aacgctggac cgccagcgca
    16681 gcatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaacc gccctggcac
    16741 gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta
    16801 atgccgatat ttatgcgctg ggcgattgca cggaaattaa cggtcaggta ttgccgttcc
    16861 tccagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc
    16921 cgctgaaact ccctgcgatg ctggtgaaaa tcaaaacgcc agaattaccg ctgcatctgg
    16981 caggcgaaac ccagcgtcag gatttacgct ggcaaattaa taccgaacgc cagggaatgg
    17041 tggcgcgcgg tgttgatgat gccgaccagc ttcgcgcctt tgtggtcagt gaggatcgga
    17101 tgaaagaggc gtttggattg ttgaaaacgt tgccggtgta ggcatggttt tctccctcgc
    17161 ccaaagggcg aggggactgt ccgcgctctt tttacgctta gttacgccac atccgccact
    17221 atgcagcatg caggatgcgc gccgcagcaa taaccccctg ccccagagac aagccgccat
    17281 cacccgccgg taaactctgc ggaaagagta atgtgaaatc agcgagataa tgcgccaggc
    17341 gtgtacgcag caatcggtta tgagtaaccc caccgctaaa taccagcgta gtgataccgc
    17401 gcatcgtggc ctgctcacgc atcaacgcgg caaaaccctg cgccagcgca tcatgaaacg
    17461 cccacgcgcg ttgattaacc ggtgcctgcc agttcagcca ctgctgccag aaagtggcga
    17521 gatccagttg attgtccacc tgcggcatcg tcaccggatg cgtcactccg tcgcatgagg
    17581 ctgcgagcgc ctccagagca caagccgctt caccttcata acttaacgtg gctggcgcac
    17641 agcccagtgc cgccgccaca gcatcgaaca aacgtccaca cgatgacgcc agcggcgcgt
    17701 taattccacg ctcaatggcc cgcgccagca cgctccagtt ttgctgttgc acacttgccg
    17761 tttcagggta attctgccac tccggcacaa agcgcaggca ctgcgccagc aggttacgcc
    17821 acggctgctt tgcagccaaa tcgccccccg gaagcgccac cgcaggcaag ccgcccaggt
    17881 gctcacattc gcgatagttc acccgcaggc attcgccgcc ccataaagcg ccgttctccc
    17941 ccataccgat accgtcgagc gtcaacgcaa tgacatcacc gccatccagt ggccagtgat
    18001 gttctgccag acacgccgcc gcatgagcat gatgatgcag caccgtttgt gtcggcagat
    18061 tcatttcgcg cgcccactgg ctggagacat agcccgggtg cgcgtcatgc acaacgtatt
    18121 gcggggtgaa atcgtagatg ttttgcatca ggcgtaacgc ttcgcgccac tgcatctgga
    18181 tgccatcgtc acttaaatcg cccagatgct gactcaacac cgcttgttcg ccgcgcacca
    18241 ggcagaaggt gtttttcaga tccgcaccga gacacagcac aggcggaaca tttttaaagc
    18301 ccggaggcaa agccagcgca tccggcacat acccccgcga acggcgcagc atttcgccgc
    18361 tttcgcgcac caccgaatca tccatccgc
//
LOCUS       DAYPHN010000094        18194 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000094 DAYPHN010000000
VERSION     DAYPHN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.094"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..2855)
                     /locus_tag="ACG0BW_003031"
     CDS             complement(<1..2855)
                     /locus_tag="ACG0BW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFK7259962.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTA"
     gene            complement(2852..3631)
                     /gene="ygfM"
                     /locus_tag="ACG0BW_003032"
     CDS             complement(2852..3631)
                     /gene="ygfM"
                     /locus_tag="ACG0BW_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFK7259963.1"
                     /translation="MIEQFFRPDSVEQALELKRRYKDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTIVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3682..5010)
                     /gene="ssnA"
                     /locus_tag="ACG0BW_003033"
     CDS             complement(3682..5010)
                     /gene="ssnA"
                     /locus_tag="ACG0BW_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFK7259964.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5013..8111)
                     /gene="ygfK"
                     /locus_tag="ACG0BW_003034"
     CDS             complement(5013..8111)
                     /gene="ygfK"
                     /locus_tag="ACG0BW_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFK7259965.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSGSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRECLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNKLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8433..9011)
                     /gene="mocA"
                     /locus_tag="ACG0BW_003035"
     CDS             complement(8433..9011)
                     /gene="mocA"
                     /locus_tag="ACG0BW_003035"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFK7259966.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEYCFLTHGDM
                     PTLTIDIFSKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNITNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRHTEI"
     gene            9114..9884
                     /gene="yqeC"
                     /locus_tag="ACG0BW_003036"
     CDS             9114..9884
                     /gene="yqeC"
                     /locus_tag="ACG0BW_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFK7259967.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            9932..11557
                     /gene="yqeB"
                     /locus_tag="ACG0BW_003037"
     CDS             9932..11557
                     /gene="yqeB"
                     /locus_tag="ACG0BW_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFK7259968.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11777..12709)
                     /gene="arcC"
                     /locus_tag="ACG0BW_003038"
     CDS             complement(11777..12709)
                     /gene="arcC"
                     /locus_tag="ACG0BW_003038"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFK7259969.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12757..14142)
                     /gene="hyuA"
                     /locus_tag="ACG0BW_003039"
     CDS             complement(12757..14142)
                     /gene="hyuA"
                     /locus_tag="ACG0BW_003039"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFK7259970.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESAIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14195..15406)
                     /locus_tag="ACG0BW_003040"
     CDS             complement(14195..15406)
                     /locus_tag="ACG0BW_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFK7259971.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15464..16660)
                     /gene="dpaL"
                     /locus_tag="ACG0BW_003041"
     CDS             complement(15464..16660)
                     /gene="dpaL"
                     /locus_tag="ACG0BW_003041"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFK7259972.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16718..17905)
                     /gene="ygeW"
                     /locus_tag="ACG0BW_003042"
     CDS             complement(16718..17905)
                     /gene="ygeW"
                     /locus_tag="ACG0BW_003042"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFK7259973.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         4223 a         4574 c         4770 g         4627 t
ORIGIN      
        1 gcggtgagta ttttctccgg tgtgaaatgc cattcgcgta accagatgcc gcaggcatcg
       61 tgaattgcgg tagcaatcgc cggagctgcg ccatttacac cgatttccga gatcgatttc
      121 gccccgaacg ggccgacttt atcgtcgctc ggcaccagca cagcgcggaa atcacgcgga
      181 atatcgccta ttttcggtgc gccataactg cgtaaatcac gcgttaacgg gtgaccttcg
      241 gcgtcataaa tgatttcttc gctcatactg tggccgatag cacgtagggt agcaccgtag
      301 atttgtccca acgctaactc tggattgacc ggtgtaccgc agtccagcaa ggcgtagaat
      361 ttatccaggc ggatttcacc cgtacgcgta ttgacggcaa cttcagcgaa gttcgcgcca
      421 tacgggaagg cgaaatcagg cgtgatataa ctgccagttc ccaccagtga accaaagccg
      481 gtgccggttt cgcctttatg ggcaatatcc ccgaaactaa cttcgccttt cttgccgcga
      541 acgacgcccg gcgttgctag ttgaacatct gccactggct cacccaacat ttgcgcgccg
      601 tggaacagaa ttttctcccg cagattttcc gctgccaaac gcgccgcgtt acccgagaag
      661 caagtaccgg acgaggcata tgcgccttta tcaaacaacg catgatcggt atcaccggag
      721 ataacatgca cgtcctgcgg tgggcagtgc agcacttctg ctgccagttt cgtcactaca
      781 gtatccagac cagtaccaat atccgcaccg ccagaatgaa cgataaaggt gccgtccgat
      841 tccagtttga tcatacagtt agcctgatcg atatccggga tacccgattt ctgcatgata
      901 atggcgacgc cgcgaccgat gtgccagtca ccattttgcg gtttcggtga agaccattgg
      961 atcatctcgc gaccctgacg caggatctct tccagtgcgc agctggcggc ggaaggaacg
     1021 gaggtcggcg ctttaccttc accgattgca ccgagaattt tcagctcttg cccttcgtgt
     1081 acccggttac gttcgataat ttccagttgg tcgatctgta actgttcagc cagttccgct
     1141 aatgccatgg tgatagcgaa gttacctttc ggtgcgccat aaccctgata agcaccattt
     1201 gggcaaatgt tgctgtagta ggtggtgacc tggaaatcga cgttatcgca cggatataac
     1261 ggcagcgaca gcgccggtcc gttacacggt acggtgagtg agtggttgcc gtaagggcca
     1321 gtgttggcgc ggaaatccat cttcactgcc gtcaggcgac catctttttt cgctcccagt
     1381 ttgacggtga ctttcgcgac gtgacgagag gtgttagcaa taaactcttc ttcacgggtg
     1441 tagcggaaca gtaccggacg cccggtcacg caggttgccc aagcgcacac ttcttccagc
     1501 aggatgtcct gtttagaccc aaaaccgccg ccaactcgct ctttaatgac atgaacttta
     1561 tgttgtttca tgcccacgag gcgcgcgacc tggcggcgta agtgccatgg tacctgggtg
     1621 gaggcgtgga taaccagacg atcgccgtcc atacgggtaa agcagatatg tgtttcagtc
     1681 gggcactgct gcgcctgcgt tgagttatag gttcgctcaa tgatcacatc ggcatcggca
     1741 aagcctttgt ccatatcgcc aatatgacca tgaatactgg cggcgatatt tttgcgaggg
     1801 cgagaaccga tcgggaagtt gatgatcata tgctcgccgc gctgggctgc atggctgtta
     1861 tcgtcttcca gagtatctgg cgcaccagca acatacacca ccggttcatc gtgcacgaca
     1921 ggcgcatctt ccgccattgc ttcgtcgatc gacattaccg gcttaagcac ttcatattca
     1981 acgtcgatga gcttcagtgc ttcgagcgca atttcttcac tttctgcgac gaccgcagca
     2041 acgcgatcgc cgacgtgacg cattttctta ccgaacatac ggcggtcaag cggtgacggt
     2101 tccggtgcgc tctgaccgcc cggggtatag tagatatccg ggcaattcag gtgagtaata
     2161 acgtgaacaa cgcccggtaa ggcttcagct ttgctgacat ccagatgagt aatcagtgcg
     2221 tgagcgtgtg ggctacgtaa cattttaatg acgcaggcgt cagccgttac gcggtcttca
     2281 acatagcagg gtttcgcctg caccattttc gcggcatcag ttttaggata atgcttgcca
     2341 atgacttcaa ggtcgtcacg gaaagtcgga gcgatatcaa tggtggcctg cggattattt
     2401 ttacgtgcaa ccgccagttc aatgacctga tagtattgct gccagccagc atcgcggcta
     2461 aacagacctg agagtgcatc gtcgatctct tcgcgggtag gtgcgtcgat gcgatcgaga
     2521 agatcggtga taatcagagc cgcagctgga tcgttataac cagactgcac cacgccaaca
     2581 tcaaccatgg cctgttgaac cagacttaac tcattccatt tgcccagaga ttctgcagta
     2641 cgaatatctg ccttctctaa ctgtgcggcg ataagcaagg acgcgttaac gatattaccg
     2701 ttaaagataa ttgcgtcaga accggcaaac ccaaagccat catcactgtt gcgtacagag
     2761 tgcattccca tgttaaacaa cagcttttgc acgttttcgc ctggattaac ggttagctcc
     2821 tgaggcgcgc catttaaagt aaagtggatg atcatacggc ttcctcccca gcctgttggc
     2881 agtcggcata caggtcggct accagaactc ccgtgatata gcgtttatag gccacgctgc
     2941 cccgcaaatc ttcctgcggg aaaatggcgt tggcgacagc ctgttcaagt gcattgcctt
     3001 ccagattttg tttttcgaca tcgtgcagac gcagggcttt actggcgacg ccatccagcg
     3061 caattcgcat accgtcgtgg tctgtcattg caacggctgc cgtcacgatg gttaaacctg
     3121 cctgagaacg gctaattttg cgggtcgcac aggtgcgata cggatcttta atgataattt
     3181 cggttagcag gcgatcgcat gggcaggcca ggtagtcctc gattgacagc gtttcgccgt
     3241 tgccaaaaac cagttcagca tccagtgcca gcaggacggg aagcagcacc gactcttcct
     3301 gccgggcggc gatttcacca ccaatggtcg actgattacg aacatggcgt gagtaaacaa
     3361 aaccgagggc ttcacgcagt gctgcaggaa taaatcgcgc atcacgcagt ggctgcaagc
     3421 gagacattgc accaatccgc agtgcaccgt tatcccagtc aacccagtcc agttccagat
     3481 cctgtaagga aatggcaatc tttttatcgg tacgggttgg tgtagcgttg agtttgctgc
     3541 ccccggcgaa ccagacggct tcatccttgt agcggcgctt cagttccagc gcctgttcga
     3601 cagagtcggg cctgaaaaat tgttcaatca tcttctttcc ttatttaacc ctcttccctg
     3661 cgggaagagg gtatttgtca tttatgccag cgcatccatc cgacgccaca tactggcagc
     3721 ggcttttctg gcttgcgcat aaatggaatc gcaatcgaag ttaaactgac ggtcttcata
     3781 gaccatcacg ccattgacca tcacgctatg aacgctgcct gagcccatac cgaaagcgat
     3841 atgcccggcg atattgtctg caagcagcgg tgtcggcgag ttgtaatcgc aaatggttaa
     3901 atcggctttg taaccggctt ccagaaggcc aaatttcgcg ccaaagttgc ggctcatcag
     3961 ttcgttgccg ttagccagtg ctttggcaaa actgtcaggc cacagcggac cacccgcatc
     4021 gcgatgttta aagaaggcaa atttcatctc ttcaaacatg tccgaaccaa tgccgtcagt
     4081 tcccaacgcc agattgcgga tgtcgctaag gtgatggttg tagccgacat ggttgttcat
     4141 gtttgaacgg gcgttatgca ccaggaacgc atcgcgctga ttgagtaggg cgatgtcatc
     4201 tttcgacaag tacagcccat gagcgaccag cgttttgctg tcgatgagat cgaattgcgc
     4261 cagtcgtgcc agcaggtctt tgccgtacca gtggtgactg taggaaacgt cgtaaaggtc
     4321 ttccgcagcg tgaatatgca aaccacgacc tgtggctttc acggcttcac gcagcatctc
     4381 cagaccggca tccggcacgg taaacggcgc gtgagcgccg atatgtgctt ccaccagata
     4441 cggctcggtt gctgctttct tcgcctcatc aatctgacgg gcgaagcgga tgttttcttc
     4501 tacaccttcc tgcaactctt tgatgccgtt gttacggtca gtagtttcaa aacaggtcat
     4561 cgcgcgcagg ccaactttta aaaatgcgtt gcgcaatgtg gagagcgacc cgccgatata
     4621 cgccggagag gcatggtgat cgataaccga tgtacatccg ctcttaatcg cttccaggga
     4681 acaaatcagt ccgctgtaat agagcgactc ttcatcaagg gcgcgatcga gtcgccacca
     4741 gagatttttc agcgttgaga tgaaatccgg gcagggggcg atgtttgcca taattccgcg
     4801 ggacagtccc gagtaaaaat ggttgtgcga gcagacaatt cccggcatca caatccgacc
     4861 atgcatctct ttgtagctgg catcggggta gcgttgcgtc agggcatcgc cgatagcgac
     4921 aatcacatca ttttcgatgg cgatatcaac gccttcctgc actttcgccg ggtgtaactg
     4981 cactgcggtg acattcttca gaatcaacat gattacacct ccacgcggcc cagcagataa
     5041 tgatgatgct ggtggacatg gctaataatg cggcacatat cgttcagctc cggtggtacg
     5101 ttgttgaact gaccttcgct gtcgatgttt aacacccagc tttggttatt cagacgtact
     5161 cgtacccggc aatcttccac aaggaagcca ggattgctgc tgttatcaaa gtcttgcgac
     5221 aggctgaaga cggtgatttt gtctttgtac ggtttaccgt tccacgggca gaactgagcg
     5281 cagttgccgc attcgttaca gtaagcgtcg aggtgcagcg tctggaaacg gttctggaag
     5341 ccagggaccg caatggatac gttggcacgg ttcgggcaga catccacaca cttgctgcaa
     5401 acgtagttac attcaaggca gcgtgcggct tcctgggcga caaacgcgtc acgatcgtcg
     5461 ctatccacca gggtgataga gatatcgcct ttgcgttgat agatttccgc cggattgacg
     5521 ttgttccagt atttatcgtt ctggtgggaa cggatatttt cccgactaag gatggcatcg
     5581 gtcgcccgac gcgcggttcc gacagcagcg acaatggagg atggtccgcg ctgtacgtcg
     5641 ccgatcataa agacgtcagt cagacgagtt tcgccattat ggtcgacgtc tggccagccg
     5701 tttttgtcca gcggcacgcc catcgcattc agggcttcag tatcctgctg ttcaccaatg
     5761 gcggtgatca ggctgtctac atgcagtgtt actgtttcgt tggtttcaac cggacggcga
     5821 cgacctttct catcaggttc gccaagcgac ataacgcgca aggttaaggt gccatcagca
     5881 tcgaaacgtt ccggattatt caggaaacgg aactccacgc cgtcgtgcaa cgcttcttca
     5941 tactcttcgc gccatgctgg catctcttgc agtgaacgac ggtaaacgac cgttgctttt
     6001 tctacgcctg gaacgcgtaa cgccgcacga gcgcagtcca ttgccgtgtt acccgccccg
     6061 acaacgacca catgtttgcc cagcttgagc gctgtgccct tgttgtattc acgcaggaag
     6121 gggagtgatt tccagacatt ttggttgtcg cccgccagtt tcacaccgct atttttatca
     6181 gtgccggtgg caatcagaac atagtggaag ccctgatttt ttaactgttc aacggtcaaa
     6241 tcgggtgagc agccatactc aaatttcacg ccgtgatcgg caacaaaatc gatatcgtgc
     6301 tgaattaact ctgcaggaat acggaactga ggaatgatat ttttcactac gccgcccgca
     6361 ttggcttcgc gttcaaacag cgtaaccgga tggcccgctc tggcaaggaa gtaacctgct
     6421 gccagacccg ccggacctgc accaatcacg gcaaccggat ggcgtgaacc agaaccggcg
     6481 ggtttgtgcc agcgttgctt atattcatcc caaccttttt ccagcgcgac ttttttcagt
     6541 tcgcggatat tcagcgcact gtcgtaatcc aggcgggtac agttgtattg gcactggtga
     6601 tcgcaaatat gaccggtaat ggcgggcagg gcgttgcgtt ggtagatgag ttccagcgcg
     6661 tcagcatagc ggtgttcgcc aagcagccga atgtattccg gaatatcttg cttgatagcg
     6721 caggcagtaa cacagggggc aacgtagcag tcggtcagcg gcaggtcttc tgccacttca
     6781 atacgctctt ctggcttcca gtgtttctgg gtgtattcca tggttaacgc gtctgctgcc
     6841 agtttgttca ggcgttcgac gtcaacatgg tcaagccccc aggcgtcgga gccttccagc
     6901 tcgcgcatgc aggcacttaa gcgcagatag ccgccaggtt tcagcaggtc ggttgccata
     6961 gtaatagggc gaataccagt atcaaaaata tcgcggatag tcagctgact ggcaccaccg
     7021 gaataagaaa tgggcagttt gccgtcaaag gcgcgagaga gaactgctgc aacattgatg
     7081 gagagcggga acagcgcacg gcctgacata tacatctctt caccaggcag tgcgccttta
     7141 ttgttgatgg tgccgagagt gttagtcagt tttacgccaa agccgagtga tttttctttt
     7201 gccagtacca tcaggcgttc cagcatttcc agcgcttgcg tcagcttgag gtcgtgatca
     7261 aatgactctt cttttaagcc tatgtaaccg aaaccgcaga catcgaggat ctcacgaaca
     7321 cgcgcgtacc ccagtaaggt cgggttgagt ttcacaaagg tattgagccc tttttcttcc
     7381 agcatgtagc ggcaaatggc ttcgatttca tgcggaggac agccgtgcat cgtggagagg
     7441 gtaacgcctt gtaccatgct ggtggggatg cgtgccggta aggcctgcaa gcattcgcgt
     7501 ttttcctgta agccgtggcg ggctaaaaat gcgtcatcct ggagtagttt attaagcgta
     7561 tcgcggtact gtgcgaattt cggatggtca gacgcgtcca tcatattatc gatgaactgc
     7621 tgcatgggcg gctgtttaat cccttcaagg ttataaccga cgctcatatt aaagataaat
     7681 gacttaccgg atcctgatgg ctggaacatc gcttccaaca gatgcagggc aaaccaggct
     7741 ttgaggtatt catcccaggc tttaagcagg gtaaattcgg tggaccattc ggtgttaaag
     7801 cactcgtctt cggcatcaat acagggcttt tccagctcca ggcggtcaag aatttggacg
     7861 gtttttagtt cgatgaagcg tccgccagtt agccaggaag tgacaatatt ttgcgcgagc
     7921 tgcgtgtgcg ggccagcggc agggccgacg ggagtggcac aggtttcacc gaagacacta
     7981 acagttttac cttttacggg tgagtaaaac tgttgctcgg gaataccaaa gattgagcgt
     8041 tgttgttggt attcatcaaa tatgcgcgtc aaaagttcct caaacggaat gggacgcata
     8101 atatccccca taaccctctc cttagcttat acagatatca ttgagtgacc ggtgttgaga
     8161 aatccggtgg tttaggggga tagagcaaca atcgcaccac tcttgccagg tttggcggga
     8221 aatataaaaa aatgtgaggc ggctcgaatc ttaggagctt tttgtgaatg aactcacagg
     8281 tgccaaatga gaattaaaat cactcctgaa aattattatt gccagcccat ttatcacttt
     8341 ttctcttttc tcaaaatcag tgattcctta tcattatgat aaagagtgct ggagggatta
     8401 aatatttgaa cgcaatcgtt taactacgtt ttctaaattt cagtatgcct ttttttcgct
     8461 gtaataaagt cgtccggagt atcaatatct aaaattattt ctgcattttc tatttcgacg
     8521 gaataatgtt ctcccataag gagtgcctga cgcatattgg tgatattggg acgctggatt
     8581 gcctgcatca ggcatggctt tgacactaaa atcggatggc cgggaatgcc attatggagt
     8641 ggcagtattg cgccatcatt tcgtaacgac cagattttac taaaaatatc gatggtgagg
     8701 gtaggcatgt caccgtgggt gagaaaacaa tattctgttt gtaccgcagg tactgcggcc
     8761 tttactgatg ttagtaaacc ctgtgcataa tctgggttgt ggataatagt gatattgctc
     8821 tggttcgcgt aacgttcgtg cagttcatta ccacgatagc cggtgactaa aataattcgg
     8881 ctacaaaact gcaacgcatt tttgatactt gtatcaagaa ttgttccctg ttgccagggt
     8941 aacatcattt tccattgccc cattcttgat gataatccgg cagcggtaat tatacagtcg
     9001 atggctgaca tagtggttcc attatttata ggggctaata acgcctgata attgcgttac
     9061 ttcctgatgg attaagttgg ggataattta tcccagagag gtcataaaga ctcgtgaaaa
     9121 gtatagttga cccatcggca ttattcattg atttaggcgc gcagaaacgt cccactgtga
     9181 tttctgttgt tggggctggg ggaaaaacca gcttgctttt ttggctggca gaactgctcc
     9241 aggctagcgg caggcgtgtg ttaatcacta cgacgacaca tatgtttatg ccaacgtctc
     9301 actggcccgt ggttttctgt cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc
     9361 ccatttcatt ttgttttcac agctggaaag cgaaccaggg aaaagtgcag ggatttacgc
     9421 cagaagcgat tgatgcactg gtgcaacgac cagagtgtga cgtaattctc attgaagcag
     9481 atggctcgcg tggaatgccg ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa
     9541 gcagttgctg cgtgattgct gtgatgggag gacatatttt gggcgcgaaa gtgagcacag
     9601 aaaatgtcca tcgctggtcg cagtttgctg atattaccgg gttaacacct gatgcaacct
     9661 tgcaactgag cgatctcgtt gcgctggttc gccaccctca gggcgcattt aaaaacgtac
     9721 cgcaaggttg tcggcgagtc tggttcatta accgtttttc tcaatgtgag aatgcgattg
     9781 cgcaaagcga gctccttcaa ccgctgcaac aacacgacgt agaggcaatc tggctgggcg
     9841 atatacaaga acatcctgca atcgcgcgca gatttgtgaa ttagcggccc gggtattccg
     9901 ggttactgaa aggatatcca ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact
     9961 cgaagagcaa aattgtccgt ttgcgatggc gcaaattgtt gatagccgag gctctactcc
    10021 ccgccattct gcacaaatgt tagtgcgcgc cgatggttct atcgtcggta caattggtgg
    10081 cggaatggtt gagcggaagg tgattgaaga gtcgcttcag gcattgcagg aacgtaagcc
    10141 gcgattattc catggacgta tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg
    10201 aggtgcaatg tcagtattta tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg
    10261 cgcggggcat gtcaaccggg cgatagccca gagtgcggcg ctattaggat ttgatattgc
    10321 cgttgctgat atttatcgcg aaagcctcaa tcctgaacta ttcccaccat caaccacgct
    10381 tctccatgct gagtcgtttg gtgcggcagt ggaagcactg gatattcgcc ctgataattt
    10441 tgtcctgatt gccacgaata atcaggatcg tgaagccctc gacaaactca ttgaacagcc
    10501 cattgcatgg ctggggttgc tggcaagtcg ccgcaaggtt cagcttttcc tacgtcaatt
    10561 gcgtgagaaa ggcgtggctg aagaacatat tgcccgttta catgcgcccg ttggttacaa
    10621 cataggtgcg gaaacgccgc aggagatcgc catcagcgtg ctggcagaaa tattacaggt
    10681 gaaaaataac gcgccgggtg ggctgatgat gaaaccttct catccttccg gacaccagct
    10741 ggtggtgatt cgcggtgcgg gggatatcgc cagtggtgtg gcgctacgtc tgtatcatgc
    10801 gggttttaaa gtgatcatgt tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc
    10861 gtttgcccag gccgtgttcg atggcgaaat gacggtcgaa ggcgtcactg cccgcctggc
    10921 aaccagctct gcggaagcga tgaaacttac cgaacgcgga ttcatccctg tgatggtaga
    10981 tcccgcctgt tcattgcttg atgaactgaa accgctttgc gtggtggacg ctattctggc
    11041 gaaacagaat ttggggacgc gggcagatat ggcaccagta acaatcgcgc ttgggccggg
    11101 ctttattgca gggaaggatt gtcatgcggt aattgaaaca aatcgcgggc actggctcgg
    11161 tcaggtgatt tactctggtt gtgcgcagga gaataccggt gttcctggca atattatggg
    11221 gcataccacc cgacgggtga tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa
    11281 attaggcgat ctggtgaaag agggcgatgt gattgcctgg attggtgagc atgaaattaa
    11341 agcaccgttg acggggatgg tgcgtggctt gttgaacgac ggactagcag tggtcggtgg
    11401 ttttaaaatt ggtgatatcg atcctcgtgg tgaaacggct gatttcacca gcgtttctga
    11461 taaagcccgg gcaattggcg gcggcgtact tgaggcgtta atgatgttga tgcatcaggg
    11521 cgtgaaagcg acaaaagaag tgctggaagt ggcttaaatg aaaatggccg gatggtatta
    11581 ccccccggcc tgagactgat gacaaacgcc aaattgcctg atgcgctacg cttatcaggc
    11641 ctacaagtgc attgcaatct attgaatttg cgatgttttg taggacggat aaggcgctcg
    11701 cgccgcatcc ggcatcaaca aagcgcactt acttgacaat ctttggccgg atggtattac
    11761 ccacccggcc tttaaattac tgacaaatca ccgttccggt tttgccttca atcccttctt
    11821 tcgctttgct caatacggta atcagtgctt cacgacctgc gcgagagcga gcaaacgagg
    11881 cggcagcttc cactttaggc agcatagaac ctttcgcaaa atgaccttct tcgataaagc
    11941 gttcagcatc acttaatgac aggcgatcga gccactgttc attctcttta ccgaagttaa
    12001 tggccacttt ttctaccgca gtcagaatga tcagcatatc ggcatcaatc atttctgcta
    12061 aacgggcgct ggcccagtct ttatcgataa ccgcactggc accacgcagg tggttacctt
    12121 cacgaataac agggatacca ccaccgccaa cggtgatcac cacctggcct gcatctacca
    12181 acgctttaac cgtttctttt tcaatgatat caaccggttt tggcgaggca acaacacggc
    12241 gatagccgcg accagcatct tctttcagag tgtaaccctg ttttgtcagt tgctctgctt
    12301 cttgctcggt aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc gggtcgttag
    12361 catcgacttc gacctgggta accagcgttg ctacaggttt attaatgcca cgagaaagca
    12421 gttcttctct taatgcgttt tgtagatcgt aaccaatata accctggctt aaggcaacac
    12481 agacagacat cggcagcatt ggcgagtgcg cttcagtttt tgcggcagct tcaaaggcct
    12541 ggttaatcat gcccacctgt ggcccgttac cgtgagtgac gatgacctca tgtccctggg
    12601 caattaaatc aacaattgcc tgagaagtaa tttttaccgc tttcatttgt ccggccaggt
    12661 catcgcccag cgcattcccg cccagggcga gaacaatttt cttactcatt tttcctcact
    12721 catcattctt gattgtgtat agatccctgg cagtggttag agcacgggag ggacaaacgg
    12781 tttgcgtcgc aggaaacgac ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat
    12841 cgtttcacca cgggataaag ttctgacaat cgcgccctga caggtaaaac cctcccatgg
    12901 cgaatagtca gcgttgtcgt ggagatggcg atgctgaatt tgttggctct gacgtgggtc
    12961 gataatcacc acgtcgccat cggaaccggg cgctaataat cctttttgcg gccacaggcc
    13021 aaacagcctg gcgggcatcg cgctggttaa ttcaacaaaa cgttccggtg ttatacgtcc
    13081 cgtcattacg ccactggaaa acagtaactg catgcggttt tccacaccgg gtaagccatt
    13141 tgggcagcga ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg agaacgtgca
    13201 gtgatcggtc gctaccacgt caatcgcacc atcgctgatg ccacaccaca gtttgtcctg
    13261 ctcgcgtacg ttacgcagcg gtgggctaag aatgaacttc atgccatctt ctgtatcgta
    13321 actgcgttcg tccaacagga gatattgtgg gcaggtttca acccagactg gctggtgatt
    13381 cgcacgggca agacgcagat aatccagacc taagccgtta gacaggtgca cgatatagag
    13441 cggggcgtta ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca
    13501 ttccagaggg cgactcaagg cgtgatagcg cggcgcggtt aaccctgcgg cgataaactc
    13561 cgcccgcttg ctggcgatag ctgcatcatt ttccgggtgc acggtggtca gcgcgccgga
    13621 ctcatgcaag cggcgtaacg cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt
    13681 taaatagagt ttaaaactgc tcagtccttc ttcgaccatc atcggaattt cgtcgaggat
    13741 ggcgtgatta atgtgctgga tcacaccgtg aaagctgtag tcgatgaccg ccttatgggc
    13801 ggcataacca cgataaacct ccagttgatg gcgtaaccgg cagccattcg ggccaaatcc
    13861 catatggtca ataatggttg ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa
    13921 atcatcacaa ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga catccacgcc
    13981 gccagggaaa acgtaacagc cagtggcatc aatttcttca tacgggagct gtggcgaaat
    14041 attgttgccc aactggcgaa caatcgcgct ttcaatcagc aaatcctgtt tggcttgtcc
    14101 atctgcgtta acgacagtgc cgtttttgat caatacgcgc atagcaaact ccaaaaaccc
    14161 tccggcgaac cggaggggaa taagtgaaga gtaattattc ggttgccagc cagcttaacg
    14221 ggattgccgc gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt ttttcgttag
    14281 gtgcatgcgc ttcaggttct ttacctgggc caaagccgat cactggaatg ccgtgacggc
    14341 ccatgataga tacgccgtta gttgagaagg tccacttatc aacaaccggc gctttgccaa
    14401 acagaccttc gtaggcattc accagtgctt taacggtgaa gtgatcttct tccactttcc
    14461 aggtcgggaa gtagcattcg gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt
    14521 tgtacataga aacaaccgcg ttagctttct gtactgcagg cagggcgcgg atttcgtcca
    14581 gcgcgccttc ccaggtttcg ccccaggtca gacggcggtc aatagagacg gcgcagctat
    14641 ctgctacagc gcaacggctt ggggatgtga agaagatttc agaaacggtg agggtgcctt
    14701 tgccgaggaa ttcgtcataa cccagacgtt gggagagttc ttgtaattcg ccaagaatcg
    14761 gacccatttt gaaaatggcg ttgtcaccgc gttctggtgc agaaccgtgg cagctaacac
    14821 cctgaacatc aatacgaatt tccatacgac cgcgctgacc acggtatacc tggcagtcgg
    14881 ttggttcggt actgaccaca aattccgggc gaatgccgga ttgttcaata atgtactgcc
    14941 agcacaggcc gtcgcagtct tcttcctgca ccgtaccggt aaccagcagg gtatattcat
    15001 cttccagacc gaggtcttta atgattttac cggcataaac catagaggcc atgccgcctt
    15061 cctggtcgga agtaccgcga ccaccgatca gttcatcagt ttccatgcct tcgtacggat
    15121 cgaagtccca gtttttgatg ttaccaatgc cgacggtatc aatatgagcg tccattgcca
    15181 ccagacgcgg gccgtggccg atataaccga gaacgttgcc catcgggtcg atttcaactt
    15241 tatcgaagcc gactttttcc atctcttctt taatacgatg tactacgcgt ttttcgtcgc
    15301 agctttcact gggaatagca accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat
    15361 cttttgcttt ttcaagaatc agtttgaatg gaatattctt agccattatt tttctccaga
    15421 ttttttactc cccgttttgc agggagcagt tccaataggg tgattaaggt gctacagcgt
    15481 gtttgccttc ccagacaact tcgcggtagt gcttcacgtc tgtgtcgcct tcagtgctga
    15541 taaccagcac tacggcatct ttgttcagcg ccagtttttc catcaggctt tgacgttgcg
    15601 ggtgataatg aaccgctgcg agaacgccca aaccgacagc gccggattca ccggagatga
    15661 tgcgcgggtc attgccgtac ggattaccca gcacacgcat acctaatgcg gcaacgctgt
    15721 cctggcagga gatgaattgg gtggcgcagt tacgtaggat ttcccagccc agcgggttag
    15781 gttcgccgca ggccaggccc gccatgatgg tggccatatc accgccaacg ttgacgatgt
    15841 cgcctttgac gccggagcga taaatacagt cagctttgtc aggttcaaca ataatgctgt
    15901 gcagattttg cgggctgtag acgtcgacca gataacccag cacaccaccg gccattgcac
    15961 cgacaccggc ttgcagcaga acgtgtgtcg gggttacgcc catttcacgc atttgctcga
    16021 cggcttcatc tgccagtgtt gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac
    16081 cttcccaggc ggtatcttgc accacttccc agccgtgctg ctgcgcgtgt tgcatggtca
    16141 ggcgaacggt atcgtcatag ttcatatccg tgacgatgca ctcggcaccg aggttcagaa
    16201 tggcgtcaac gcgttcctga gcagaacctt tcggcatgta aatcaccgca ttctgtccga
    16261 gttgctgtgc tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag
    16321 tcattttttc gccgatggca tttttcaggt gctcaaatga cagcgtttcg atatcaagat
    16381 gatatttttc gcacaataat tgagcgatgg cgtatgcacc gccgagcatt ttgaaggcgt
    16441 tcagaccgaa tcgttttgat tcgtctttga cgagaatttt cttcacacca aaaaggtttg
    16501 cgagatcgtc cagcgcacaa agcggtgttg ggcgataacc agcaattttc tggtggaact
    16561 ggcgcgccag tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt
    16621 tgttatcggc gatatcaatc ttcaatgaga aaacggacat atcctatcct tttggcctct
    16681 ggaaatagag ggccgaccag ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt
    16741 ctttcagcag ttgctcgagc agtgcacctg gtttggcgta tttacgggac aggatcatcg
    16801 cggcgatgat ataaggcttc cagctggctt ctttgtaggt cgcgatacgg tatttttcga
    16861 atacgccttc agtcacttca ccttctttgc aggatacgcc gctgatatca gctggcaggc
    16921 agtgcatgta cagggcttcg ccatcacggg tcagttccat catctcttca gtacaatgcc
    16981 agtctttgtg ttgcgcgttc tgtgccagac actgtttttc cagtgctttt aagccttcgt
    17041 gatcgttcgc acgcagcaat tcagtacgct cttccatcac tttgtaaggt gcccatgact
    17101 tcggataaac gatgtctgcg tctttgaagg cttcttccat gctggtgacc tgacggaagc
    17161 taccgccgga ggctttagca ttgtttttag cgacttcaac gacatccggg atcaggtcgt
    17221 agccttccgg atgggccagg gtgacatcca taccgaagcg agtcatcaga ccgatgatgc
    17281 cttgtggtac agagagcggt ttgccatagc ttggggagta ggcccaggtc atggcgattt
    17341 ttttaccttt caggttttcc agtgaaccaa agtgttcacg taaccatgcg aggtcagcca
    17401 ttgactgagt cgggtggtca atatcgcatt gcaggttcac taaagccgga cgctgcggca
    17461 gtacgccctg cttgtagccg tcatcaagtg cagcgccaac ttcacgcata taagcgttgc
    17521 ctgcgcccag atacatatcg tcacgaatac cgatggcatc ggcgcagaag gagatcatat
    17581 tggcggtttc acgcacggtt tcgccgtgag cgatttgtga tttgccttcg tcgagatctt
    17641 gctgtgcgag gccgagcagg ttaagcgcgg aagcataaga gaagcgggta cgggtggagt
    17701 tgtcgcggaa tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag
    17761 cacgcagtgc ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct
    17821 gttcccacgt taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct
    17881 taatcagctc attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagag
    17941 gtttatttca ctgtgatttg ccaaaagtga ttggtctttt cacagctata cctgggacta
    18001 acaataacta tgccagaatt atccaagcgg ctgcaaaaga ctaatttgac gggtaatgtg
    18061 tggggttgat cacaaattga tgccagcttc tggttgtttg taaaatacga aaacgtttgg
    18121 ctttttttat cgcgttattt atttgctgct aagtatcaga aataagataa aggggcaggt
    18181 tatccctcgt cagt
//
LOCUS       DAYPHN010000095        18145 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000095 DAYPHN010000000
VERSION     DAYPHN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.095"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            144..1130
                     /locus_tag="ACG0BW_003043"
     CDS             144..1130
                     /locus_tag="ACG0BW_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFK7259974.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            1175..1678
                     /locus_tag="ACG0BW_003044"
     CDS             1175..1678
                     /locus_tag="ACG0BW_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFK7259975.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            1678..2979
                     /locus_tag="ACG0BW_003045"
     CDS             1678..2979
                     /locus_tag="ACG0BW_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFK7259976.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWSMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDSVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(3035..4042)
                     /gene="yggM"
                     /locus_tag="ACG0BW_003046"
     CDS             complement(3035..4042)
                     /gene="yggM"
                     /locus_tag="ACG0BW_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFK7259977.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTTKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIVACHKQSAECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(4158..5204)
                     /gene="ansB"
                     /locus_tag="ACG0BW_003047"
     CDS             complement(4158..5204)
                     /gene="ansB"
                     /locus_tag="ACG0BW_003047"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFK7259978.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(5380..6099)
                     /gene="yggN"
                     /locus_tag="ACG0BW_003048"
     CDS             complement(5380..6099)
                     /gene="yggN"
                     /locus_tag="ACG0BW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFK7259979.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(6120..6260)
                     /locus_tag="ACG0BW_003049"
     CDS             complement(6120..6260)
                     /locus_tag="ACG0BW_003049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259980.1"
                     /translation="MQNCLMRYAYQAWKDAQSSRPDKAFTPHPAWMTCTLLSIWGQQM
                     LA"
     gene            complement(6283..6609)
                     /gene="yggL"
                     /locus_tag="ACG0BW_003050"
     CDS             complement(6283..6609)
                     /gene="yggL"
                     /locus_tag="ACG0BW_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFK7259981.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEEHKLEEVRTSEL
                     FDVWWD"
     gene            complement(6609..7328)
                     /gene="trmB"
                     /locus_tag="ACG0BW_003051"
     CDS             complement(6609..7328)
                     /gene="trmB"
                     /locus_tag="ACG0BW_003051"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFK7259982.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            7489..8541
                     /gene="mutY"
                     /locus_tag="ACG0BW_003052"
     CDS             7489..8541
                     /gene="mutY"
                     /locus_tag="ACG0BW_003052"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFK7259983.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHSG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVDRFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHDDEVLLAQRPPSGLWGGLYCFPQFADEESLRLWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8569..8844
                     /gene="yggX"
                     /locus_tag="ACG0BW_003053"
     CDS             8569..8844
                     /gene="yggX"
                     /locus_tag="ACG0BW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFK7259984.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            8908..9987
                     /gene="mltC"
                     /locus_tag="ACG0BW_003054"
     CDS             8908..9987
                     /gene="mltC"
                     /locus_tag="ACG0BW_003054"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFK7259985.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            10189..11445
                     /gene="nupG"
                     /locus_tag="ACG0BW_003055"
     CDS             10189..11445
                     /gene="nupG"
                     /locus_tag="ACG0BW_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFK7259986.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11494..13629)
                     /gene="speC"
                     /locus_tag="ACG0BW_003056"
     CDS             complement(11494..13629)
                     /gene="speC"
                     /locus_tag="ACG0BW_003056"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFK7259987.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVVDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(13792..13869)
                     /locus_tag="ACG0BW_003057"
     CDS             complement(13792..13869)
                     /locus_tag="ACG0BW_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259988.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            14022..14729
                     /gene="yqgA"
                     /locus_tag="ACG0BW_003058"
     CDS             14022..14729
                     /gene="yqgA"
                     /locus_tag="ACG0BW_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFK7259989.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTADPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            14835..14910
                     /locus_tag="ACG0BW_003059"
     tRNA            14835..14910
                     /locus_tag="ACG0BW_003059"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14868..14870,aa:Phe,seq:gaa)
     gene            15108..16373
                     /locus_tag="ACG0BW_003060"
     CDS             15108..16373
                     /locus_tag="ACG0BW_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7259990.1"
                     /translation="MALTDAKIRATKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            16629..17672
                     /locus_tag="ACG0BW_003061"
     CDS             16629..17672
                     /locus_tag="ACG0BW_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFK7259991.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            17815..18045
                     /locus_tag="ACG0BW_003062"
     CDS             17815..18045
                     /locus_tag="ACG0BW_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7259992.1"
                     /translation="MNYPMDSLTPFHDALLSNTLWLTSKKTGFPLPAGNHLPGVFGWS
                     GYSCLVISKIIAINSGKLIVHNLVMNITLIFP"
BASE COUNT         4520 a         4536 c         4278 g         4811 t
ORIGIN      
        1 attaatattt aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagac
       61 aggttaacca atttgtcgca caaagcactg ataagcacaa ttagatatca accaatttcc
      121 attcttcaac agggagtttt tttatgaaaa tgaggtcatt tacccgctca ttagtctgcg
      181 catccttgct ggctctggtt agcactggcg taaacgccgc agaaaaagtg acgttaaagt
      241 tagcacacaa cctggaaaga agtcatgttg tacatcagag ttttgaagag ctggcgaaag
      301 aagtaaaaca gctgtcaaaa ggtaatatgg tgatccgtat ttaccccagc agccagatgg
      361 gtaatgcgcg agaaactatg gagttgttgc aaaatggtgc actggatatg accaaaggtt
      421 ccgccagtga tctggagtct tttgataata tttatgctat ctataatttg ccgttcttat
      481 ttaaagatca ggcgcacttc aacaaagtgg tttttggtga agttggtaaa gaaattatgg
      541 attcaacaaa agataaaggc ttttttgctt tatcagctta tgtagcgggt acacgcagtt
      601 tctatgcgaa aaaaccgatt actaagccag aagatcttaa agggctgaaa attcgtgttc
      661 agccaagccc gaccaccatc aaaatgattg aactgatggg tggctcaccc actccaattt
      721 catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca gaaaataacg
      781 tgccttcctg ggtacagact cgacatatcg aaattgccaa agtcttttct gaagatgaac
      841 atgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa ttaaccccag
      901 aacagcaaca aatcctcgaa acagcggcga agaaatccga agcttatcaa caaaaattgt
      961 gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatgggtggt gaaatcgtta
     1021 aggttgataa agccccattc cgtgccgcag ttcaacctct gtttgacgac ttcaaaaaag
     1081 atccaaaaca agctgctttg ttagagaaat tcgacaacgc tgctcaataa tatttcgggc
     1141 cagtctctgg ccctgatttt ccaggatgct tacaatgata tttaaccgcc tgaaacttgc
     1201 ggtagaccgc gtcattgccg cgttttctgt cgccgtaatg cttgctctgg ttgtatgcgt
     1261 agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa ccgatgagct
     1321 ggcacgcttt ttgatgatct gggtaggatt attaggtgct gcatataccg tcggcgcgca
     1381 gagacatctc tctatcgatt tatttgctct cgccctgaat aagcgcaagc aattacttct
     1441 tagtatagtc attaacgttc ttattttagg ttttgccggt tcggtcattg tgactggtgg
     1501 cctgaaatta attgataaaa cactggcaac atcacaggta tctgccgcaa tgcaaatacc
     1561 gatgggatac gtatatatca ttctgccatt aagtggattg gtgatgatgt tttatgcctt
     1621 gtgttttatt aatcaaagca ttcaacaact gaaacaacct gcgcaggagg ccagttaatg
     1681 gatagttata tcgcgattac actgtttggc tgtttttttg tgctggtctt tattggtgtg
     1741 cccatttcgt tctcaatcgg tattgccact gtagcttcaa tgcttttgat gtttccctgg
     1801 gatatcgcgg caatcactgt ttcgcagcga ctggctaatg gactggataa ctttgcccta
     1861 ttggcaatcc cctttttcat ctttgccgga acgctgatga acagcggcgg tattgcgata
     1921 cgtttgatta acctcgccca ggtaatggtt ggtcgggttc ctggttcact tggtcatgtg
     1981 aacgtcctgg cgaatatgat gtttggttcc atttctggtt cagccgttgc ggcagctgct
     2041 gccgttggtg gcacactgaa tcccattcag accaaacaag gttatgatcc ggcattttct
     2101 accgctgtaa acgtctcatc ctgtattact ggattactga taccgccctc aaacgtgtta
     2161 attgtgtttt ccctgacagc tggcggcgtt tctgtagcat cgctatttat ggcgggatat
     2221 ttgccaggga tcttaatggg tcttgcggta atgatagtct gcggcattat tgctaaacgc
     2281 cgtggctacc cactttcaga aagagcaacc tttgctcagg cctgtaaagc gtttctggat
     2341 gccttaccaa gtttgcttct ggtgttcatt gtaatgggcg gcattctggg cggcattttt
     2401 accgcaactg aagcgtctgc gattgccgtt gtctacacat ttattctctc ggtgctgatt
     2461 taccgggaag taaaatggcg cgatttaccc aaactgattc tggaatcggt tgttaccact
     2521 tcgattgtat tactgttgat cggtttttcc gtgggcatgt cctggtcaat gaccaatgct
     2581 gatattcctt atatgatcag cgacgcgttg atggggattt ctgataaccc actgatcata
     2641 ttgctgctga ttaatatcgt cttattaatt gtcggcatct ttatggacat gacaccggct
     2701 gtactaatct tcacaccaat cttcttgccg attgcacaag agctgggaat ggattcggta
     2761 catttcggta ttatgatggt ggctaacctt tgtattgggc tgttaacacc gccggtgggt
     2821 agtgcgctat ttgttggttg ttctatttca ggtgtgaaga ttcaacattt gatcaaacca
     2881 ttgctgccat tttatgccgc attgttaatc gcgttgatga tgatcactta catcccgcaa
     2941 atatccttgt tcattccaca gctactaggg ctaatgtaaa aaataatttg ccggataact
     3001 ttttatatta tccggcaaag gtacttaaca tgagttactt aatcactcca ttgcgccgca
     3061 attgttcggt atcttttttc cagcgttcat tagcctggtt gatatcatca attttcatca
     3121 aaatcgcgac ttttttactg tttatttctg aaactttcat cgttaacgga tattgccctt
     3181 tttcgagcgt tatccagtca tcctgcaatt tacggctaag tttttgtgat tgcagaaaag
     3241 tctgtcgccg ttgttcgttg atatcgaaat ctcgcttctg ctgaattggc acttcataac
     3301 actctgcact ctgtttatga catgccacaa tcgccggttg ataaacctct ttgtcatatt
     3361 taaccgcgaa agcctcgcta tcgttctgtt tattaaactc atcacgctgt tggtgaattt
     3421 ttttaaaggc gtcttcgcgg gtcatttgct taccgtttgc atcttttccc caatatgcat
     3481 ccgctgcttt ctgcgccgct accatttgct tatcaagcgc tttcacttcc ttttccagcg
     3541 caggaatcga gttcttctgg ttattcaacg ctgattcaag ctgactaaag cgataattga
     3601 aatcttcgct attcacgatc agatatttat tattcgtttt gatatcgtcg accacccgcc
     3661 cacgatcgct ggctgccgcc tgaaaagagt aaaagttcac cgaccagcca gacgcgggcg
     3721 ttcctttagt ggttaacatc gccgagaatt ttatcggttt atctttcgtc caggtctgtt
     3781 cgagcagttc gtaatctgcc aactgaccga cccaggtata gagatcgtca ctggaagaga
     3841 catcgccttc cgctgaccag gtggcctgat tacccttagc atcaagattt tttaaggtga
     3901 tgacatcaag cttgagaatg ccgtgatact gctttttgaa ttgatctttt aagatatttt
     3961 cagttggcgg ttcgccatct gcccagacat taccagtcat caacataagt agtgccgtgc
     4021 caacaatcca ttgctttttc acccgaaaac cctcacgagg aataatttat tccggtgatt
     4081 atcatcaggg taatgaaaat tggcaatgga cgtaagtctg aagtgaaaag ccccggcacg
     4141 ataccggggc gaggtgatta gtactgattg aagatctgct ggatctgctg cggatctttg
     4201 gtttgcgtca gagcaagctg cagcagaacg cgcgcttttt gcgggttcag cgtgccagag
     4261 gcgacgaagc cgtatttcgc atcatccact tcagcatcct gagtggtagc gcccgtcggt
     4321 acgcgggaag aacgcacgac tgcagtgccg tttttcgcgg cggtcgccag agtgtcaaat
     4381 acggttttat acaggttgcc gttacccaca ccagcgctaa caatgccatc atagcccgca
     4441 tctaccagtg ctttagccgg aagatcggat gcgttagcgt agttataaac aatgccgact
     4501 ttcggcagtt cattcagctt agagacatcg aacggcgtgt cgctggtgtg cttacgtgcc
     4561 ggggtacgtt ggtagtcaat cttaccgttg tgaatgtaac ccagaggacc gtagttaaca
     4621 gacttgaagg tcgctacgtc ggtggtgttg gttttggtga catcgcgacc gtccagtacg
     4681 gtgtcgttca tcaccaccag cacgccacga ttagcggagg ctttatcagc tgcggtcact
     4741 accgcgttat acaggttgaa tggaccgtct gcgctcatgg acgtggacgg gcgcattgcg
     4801 ccgaccatca ccaccggttt gtcgcatttc accgtcaggt cgaggaagta agcggtttct
     4861 tccatcgtgt cggtaccgtg ggtaatgacg aagccgtcgg ttttatcgca gtcggtgtta
     4921 attttcttcg ccagtgtcag ccagacatta tcgttcatgt cctgggagcc gatattcact
     4981 acctgctcgc cttttacatt cgcaatgtcc ttcagttgcg gcaccgcatt aaccagattt
     5041 tctacgccaa ctttacccgc tgtgtagtta gatttggttg cggagtcacc accaccggca
     5101 atggtcccgc cggtcgctaa aatggtgata ttgggtaatg ccaatgctgc accactaaaa
     5161 cccataacca gtgcggcaag tgccgtcttt ttgaaaaact ccatttcatt cctccagtta
     5221 cgtgaacgct acgcattatc ccttagcttt gcatgggaaa tttgacgtta aacaatttac
     5281 aacgtgaata tattttggag atctacaaag ttagaggcag gtaacaaaac gaagaattaa
     5341 acggcataaa aaagtattat gccgtcttaa aatagaggat tattttaaat tcccgaccag
     5401 ggctttgcga ctatcttcca gagtcacaac gcggctacaa acatctttgc caaactgttg
     5461 gaaatctttt tcctgctttt tccattcggt ttggattgag gattgcagac cgcccaggct
     5521 tcccagcaca ttctgtaatg ggttaccgcc gcttttcagc accgctttcg cgcccatttc
     5581 attaatgctg tcctgtaaaa ttccgcccat tgcctgattc actaattgct ggccttcggc
     5641 gcgaacctga tcaatggctt tatagtgaaa cgtcaggcca tcgctgcgcg tttcgataat
     5701 gcggttcatc tgctctttca gctgcgcatc aagtttggtc agacggctgc gcattttgct
     5761 gctttcgccc atctcctgaa cgataatttt atccagcgca atacgggctt tctcgacgcg
     5821 gcttttcgcg ccttcatcaa tccacggcag cgtgctacgc agttcagcct gataatcctt
     5881 cgcctgctcg cgctgggcgg cattcaggga atattgctta ccgttataca tcacgttgcc
     5941 gtctggcgtg atcaccagat tgccgttttc gcccttcacc tgcacggttt gcgggctgac
     6001 aatcacatcg tcacgcggcg tgacgctgca ctggtagtcg gcgtgagcgg tcattgccgt
     6061 cactgaaagt gctgccgcca gcagcatttt gcgcatcata gtcttccctc aagaacaaat
     6121 caggccagca tttgctggcc ccagattgat aacaaagtac atgtcatcca tgccggatgc
     6181 ggcgtaaacg ccttatccgg cctactcgat tgtgcatctt tccaggcctg ataagcgtag
     6241 cgcatcagac agttttgcat ttgtcatcgc ccgtatgctt tcttagtccc accaaacgtc
     6301 gaaaagttcg ctggtgcgta cctcttccag tttgtgttct tccagccact tacgcacaat
     6361 cgcctgatgc tcttcggtgc atttgccgat ttcctgcatg cagatcagac cttcccaggc
     6421 cagataaccg ctgccgtcaa aggccagttt gttcggttcg ataacctcgt taataaagtc
     6481 atcaacggtt ttatcaatct gttcttccga tgtaccttcc gggaatcgcc atgccaccga
     6541 aaatcctaat tcctggaatt cgtcgatgtg cattttttta cgcagacgac ggctacggtt
     6601 ctttgccatt atttcaccct ctcgaacatt aagtcccata ctccgtgacc aagacgatga
     6661 ccacgttgtt caaatttcgt caccggacgt gatgccggac gcggtacgta atcattgctc
     6721 tctgacaggt ttttataacc gtcaatagaa gacatcactt caagcatatg ttccgcataa
     6781 ggttcccagt cggtcgccat atggaatacg ccccccagtt gcagtttgct ttttaccagt
     6841 tcggcaaacg gcacctgaac gatacggcgt ttattatggc gcgctttgtg ccacgggtca
     6901 gggaaaaaga gctgcaccat gcgcaatgaa ttgtcaggaa tcattttatg cagcacttca
     6961 accgcatcgt gacacatcac gcgcaggttg cttaagccct cttcatgcgc agaagccagg
     7021 cacgcaccaa cgcccggtga atgcacttca atgccgagga agtcctgctc ggggcgatct
     7081 ttagccattg ccaccagcga cgcccccatg ccaaaaccaa tctcaagcgt caccggcgct
     7141 tcacggccaa aaagcgcagg gaaatccagc atatcttcgc tgaactcaac gcccatcacc
     7201 ggccagtagt tttccagcgc atgttcctgg cctttggtca gtcgcccctg gcggcgcaca
     7261 aaactacgga tacggcgcag tgggcggccg ttttcatcaa attccggtga gatgacgtcg
     7321 tttttcataa agatttagtc gcttgtgaaa gtgttctgaa aacgggcatt atccaaagtt
     7381 agttgccgga tgcaagcatg ataaggccgt ggctgcggaa agttccggtt tacaccctgc
     7441 cgtcgctgtg ctgcaatctt gcccccaaca acagtgaatt cggtgaccat gcaagcgtcg
     7501 caattttcag cccaggttct ggactggtac gataaatacg ggcggaaaac gctgccctgg
     7561 caaattgaca agacgcccta caaagtatgg ctctcagaag tgatgttgca acaaactcag
     7621 gttgcgaccg ttatccccta ttttgaacgc tttatggtgc gcttcccaac ggtgaccgat
     7681 ctcgccaatg cgccgctgga cgaagttctc cacttgtgga ccgggcttgg ctattacgcc
     7741 cgcgcgcgca atctgcataa agcggcacaa caagtggcca ccttacacag cggtaaattc
     7801 ccggaaacct ttgaagaagt cgcggcgtta ccgggcgtcg ggcgttccac cgcaggcgcg
     7861 attctctcgc tttctctggg taagcacttt ccgattctcg acggtaacgt caaacgggtg
     7921 ctggcgcgct gctatgctgt aagcggctgg cctgggaaaa aagaggtcga gaataaactg
     7981 tggagtttaa gcgagcaggt gacgcccgcg gtcggcgtgg atcggtttaa tcaggcgatg
     8041 atggatttgg gcgcgatgat ttgcacgcgc tcgaagccga aatgttcgct ctgtccgcta
     8101 caaaacgggt gtattgccgc cgccaacaat agctggtcgc tttatccggg caaaaaacca
     8161 aagcagacgc taccggagcg cacgggttac tttttactgt tacagcatga tgatgaggta
     8221 ttgctggcgc agcgcccgcc gagtggattg tggggcggtt tatactgttt cccgcagttt
     8281 gccgatgaag aaagtttgcg actatggctg gcgcaacggc agattgctgc cgataacctg
     8341 acgcagctga ccgcgtttcg gcataccttc agccatttcc acttagatat tgtgcctatg
     8401 tggcttcccg tgtcgtcatt caccggctgc atggatgaag acaatgcgct ctggtataac
     8461 ttagcgcaac cgccgtcagt tggcctggcg gctcccgtgg agcgtttgtt acagcagtta
     8521 cgcactggcg cgccggttta gcgcgtgagt cgataaagag gatgatttat gagcagaacg
     8581 attttttgta ctttcctgca acgtgaagca gaaggtcagg attttcagct gtaccccggc
     8641 gaactgggaa aacgcatcta taacgagatc tccaaagaag cctgggcgca gtggcagcac
     8701 aaacaaacca tgctgattaa tgaaaagaaa ctcaacatga tgaatgccga gcaccgcaaa
     8761 ctgctggaac aggagatggt caacttcctg ttcgagggta aagaggtgca tatcgagggc
     8821 tatacgccgg aagacaaaaa ataaaaacag tgccggagca cgcctccggc aacttgcata
     8881 aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc tttgattgcg
     8941 ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga agcctgggtc
     9001 aaagacacca acggttttga tattctgatg gggcaatttg cccacaatat tgagaacatc
     9061 tggggattca aagaggtagt gatcgccggt cctaaggact acgtgaaata caccgatcaa
     9121 tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga aaccatcgcc
     9181 gggacagaac ctgccgcgca tttgcgccgg gcaataatca aaacgttgct gatgggggac
     9241 gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc gaaagaacct
     9301 ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga aggtcgcgcg
     9361 agcaacttcg cggattatct gctgaaaaac cgtctgaaga gccgcagcaa cgggctgcgt
     9421 atcatctaca gcgtcaccat taacatggtg ccgaaccacc ttgataaacg tgcgcacaaa
     9481 tatctcggta tggtccgcca ggcgtcacgc aaatatggcg ttgatgagtc gctgattctg
     9541 gcaattatgc agactgaatc ttcctttaac ccgtatgcag tcagccgttc cgatgcgctg
     9601 ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc gcaggggaaa
     9661 tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga taccggcacc
     9721 gcgtatctgg cgatgctgaa caacgtttat ctcggcggaa ttgataaccc tacatcccgg
     9781 cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg agtcttttcg
     9841 aatgacaaga ttcaggcagc caatattatt aacaccatga cgccgggcga tgtttatcag
     9901 acgctgacga cccgccatcc ttctgcggaa tctcgccgtt atctttataa agtgaatacc
     9961 gcgcaaaaat cctaccgccg ccgataattc cactaaccgc ccctgacgat gctcaggggt
    10021 aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc aattatttgc
    10081 cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc gcatcatctc gcacggtcaa
    10141 atgtgctttt tcaaacactc atccgcatca cgatgtgagg aaattaacat gaatcttaag
    10201 ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg gctgacgacc
    10261 ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg cgcagtttat
    10321 agttcactgg gtatcgccgc ggtctttatg cctgcgctgc tggggattgt ggccgacaaa
    10381 tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat cacgctgttc
    10441 atggcggcac aggtcacgac gccggaagcg atgttccttg tgatattgat taactcgttt
    10501 gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct gcaaaatgcc
    10561 gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat cggctttatc
    10621 atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca gctgtatatt
    10681 ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca tattccggtt
    10741 gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc attcgcgctg
    10801 tttaaaaaca agcgtatggc aatcttcttc atcttctcaa tgctgctggg cgcggaactg
    10861 cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga tccgatgttt
    10921 gccagcagct tcatcgtgca gcatgcgtca atcatcatgt cgatttcgca gatctctgaa
    10981 accctgttca ttctgaccat cccgttcttc ttaagccgct acggcattaa gaacgtaatg
    11041 atgatcagta tcgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg tgaccctact
    11101 ccgttcggca ccgtactgct ggttctgtcg atgattgttt acggctgcgc attcgacttc
    11161 ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat tcgcgccagt
    11221 gcgcagggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg cggcatcgtg
    11281 agcggtaaag tggttgagat gtacacccaa aacggcatta ccgactggca gaccgtatgg
    11341 ctgattttcg caggttactc cgtggttctg gccttcgcgt tcatggcgat gttcaaatat
    11401 aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg caaagaaaaa
    11461 cgggtcgcca gaaggtgacc cgttttttta ttcttacttc aacacatacc cgtacaaccg
    11521 tttcatgcca tctgcatcgg tttcgctata gacaccttgc agctccggcg aaaatcccgg
    11581 caacaaattc accccttctt ccagcgcaag gaaataacgt tgaaccgccc caccccagac
    11641 ttctccgggt accacgcaaa gcacgcctgg tggataaggc aacgcccctt ctgccgcaat
    11701 tcgcccttcg gcatcacgaa tccgcaccaa ctccacttca ccgcgaatat aagcgctatg
    11761 cgcatcctgg ggattcatca ccactgacgg gaaactctgc tggcggaaca tcgctttttg
    11821 taggtctttg acgtcgaaac tgacatacag atcgtgcatc tcctgacaca actggcgcag
    11881 ggtgtagtcg cgataacgca ccggatattt gttataaacg ctcggcaaca cctcaaccag
    11941 cggcgagtca tcctcaatat gctgttcaaa ctgcgccaac atcgccacca gttgtgccag
    12001 cttctcgtgg ctttccgccg gagttaataa gaacagaatg gaattgagat cgcacttctc
    12061 cggcacaatg ccgttctcac gcagatagtg cgccagaatc gtcgccggaa cgccaaagtc
    12121 gctatattcg ccagtttcgg cattgatacc tggtgtagtg agtaacagct tgcacggatc
    12181 aacaaaatac tgatccgcgg catatccttc aaagccgtgc cacttcgccc ccggctcaaa
    12241 actgaaaaaa cggcggtcgc tggctaatac tgacgtcgga taatcctgcc acaatttgcc
    12301 atcaacaaca ggcgggataa acgggcggaa cagcttacag cgcgcaagaa tagccttgcg
    12361 cgcttcaatc cctatctcaa cacactcagc ccacagccga cgcccactct ccccttcatg
    12421 aattttggcg ttaacgtcca gcgcggcaaa cagcggatag aacgggctgg tagaagcatg
    12481 gagcataaag gcattattta accgcttatg cgggcaaaaa cgcgcctgcc cgcgaatatg
    12541 gttatctttt ttatggatct gcgatgtctg cgagaagccc gcctgctgtt tatgcaccga
    12601 ctgcgtcaca aagatccccg gatcattttc gttaagctct aacagcagcg gcgagctatc
    12661 tgccatcatc gggataaact gttcgtaacc gacccatgcg gaatcaaaca gaatgtaatc
    12721 acacagatgc ccaacggtat cgaccacctg acgggcgtta tagacagtgc cgtcataggt
    12781 tcccaactga ataatcgcca ggcgaaacgg gcgcggcaac tcagcttttt ctggcgcaac
    12841 gtcgcgaatt tgctggcgca gatactcttc attaaaacag tgcgcatcaa taccgccaat
    12901 gaaaccaaac gggttacgtg aagcttccag atagaccggc gtcgcccccg cctgaatcag
    12961 cgcgccgtga tgattcgact tatggttgtt acggtcgaag agcaccagat cgccacgcgt
    13021 taacagcgca ttcgtcacca ctttattcgc tgccgatgtg ccgttcagta caaaataggt
    13081 tttatcggca tgaaagactt tggctgcgaa tttctgcgca tccttcgccg atccttcatg
    13141 aatcagcaga tcgcccaatt ttacgtcagc gttacacata tcggcgcgaa agatgttctc
    13201 gccaaagaaa tcgtaaaaat ggcgtccggc aggatgcttt ttaaagaacg caccatgttg
    13261 atgtccaggg caagcaaagg tgctgttgcc catctcaacg tactgcgtca gcgtgtcata
    13321 aaacggtggc agcaaattct cttcatactg acaggctgcg gattccagct ccagccactg
    13381 ctgctcgttg ccgttgatta ccgccgtaac gcccgcaggt aattcaacaa catgttcgga
    13441 atacaaaaac accggtagat gaaaaccggt gcgcttaagc aacgcaagaa tgccactgcg
    13501 actatccgca gcggtaatga cgactgccgc gacgtccgta aaatcagtat ctgccaacgc
    13561 caccacgcgg cgatgagtag aaagtcggga taccagttca ctactggcgg caatattcat
    13621 tgatttcata agcgcaaaac ccgtttcggg gaagtaagaa taccggacaa ggtgaaaaac
    13681 cctgccccac gagatatggg tcaaactggt caccagctcc gaccgccaga catcagtaaa
    13741 agcagaaacg ctctgatttt actgttgtcc tgcagtgagc gtgcgttaac ttcaccgcat
    13801 gagcagaaaa agggaggagt ttcgcgcgaa acggcgataa gcgagagaat gtaaggaggt
    13861 ggcgtgcatc gggcaaactc cgtacagaga ggagaaaatt cgcgcaatca tggcaccttt
    13921 cgctaaagcg tgcaagccag gactttgcga ccagacaagc catcaacaag gcaaaaagtt
    13981 cataataaga aaatcaaaca atacagttaa caggaactct tgtggtcatc ggccctttta
    14041 tcaacgctag tgcagtctta ctgggtggcg ttctcggcgc actgctcagc caacgcttac
    14101 cggaacgtat ccgcgtctcc atgacatcaa tttttggtct ggcatcgctg gggattggta
    14161 ttttactggt ggtgaaatgc gccaaccttc cggcgatggt tttagcaacc ttactcggtg
    14221 cattaatcgg cgaaatttgt ctgctggaaa aaggcgtcaa taccgcggtc accaaagcac
    14281 aaaatctgtt tcgccactca cgtaagaagc cagcgcatga atcttttatt cagaattatg
    14341 tcgcgattat tgtcctgttt tgcgccagcg gcaccgggat cttcggggcg atgaacgaag
    14401 ggatgaccgc cgatccgagt attttaatcg ccaagtcatt tcttgatttc tttacggcga
    14461 tgatcttcgc ctgctcgctg ggtattgcgg tatcggtgat tagtatccca ttactgatca
    14521 tccagttaac gctggcgtgg gctgccgcgc tgatattacc gctgaccaca ccgtcgatga
    14581 tggcagactt cagcgccgta ggcggtttat tgctgctggc aaccggatta cgcatctgtg
    14641 gcattaaaat gttcccggtg gtcaacatgc ttccagcact ccttctggca atgccccttt
    14701 ccgccgcctg gaccgcctgg tttgcctgac aatgcgtgca ttagcggcaa agtgatgata
    14761 gattgtgcag tctgcagtaa attgaagaaa tttgattgac gagacgaggc gaatcaggtt
    14821 taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa aatccccgtg
    14881 tccttggttc gattccgagt ccgggcacca agttcatatc aacggacctc cacggaggtc
    14941 cgtttttcgt ttcagaacgc cacaatttaa gcgttctgcc tccaaatcaa ttctaccgaa
    15001 ctcaaccaga ttccccccac atcaacccca ttgtgtgggt ataattgcgg gtatacccca
    15061 gttcgacaga atttgtaccc tctttaacgc attcagaagg ctgaactatg gcactgactg
    15121 acgcaaaaat ccgggctaca aagcccactg acaaggctta taaactcact gacggggctg
    15181 gcatgttcct gctggtacat cctaatggtt cccgttactg gcgtctccgt tatcgtattc
    15241 tgggtaagga gaaaaccctg gcacttggtg tgtatccgga agtttctctc tccgaagctc
    15301 gtacaaaacg ggatgaggcc cgaaaactga tttcggaggg ggttgaccct tgcgaacaga
    15361 aaagagctaa aaaagtagtc cctgatttac agctctcttt tgaacatatt gcacgacgct
    15421 ggcatgccag taataaacaa tgggcacaat cacacagcga taaagtactc aaaagcctcg
    15481 agactcacgt tttccccttt atcggcaacc gggatatcac aacactcagt accccggatc
    15541 tgcttatccc tgttcgtgct gcagaagtta aacaaattta tgaaatcgcc agtcgtctgc
    15601 agcaaagaat atctgctgta atgcgttatg ccgtacagtc tggcatcatc agatataacc
    15661 cggctctgga tatggctggt gcattgacca cggtaaaacg ccagcatcgc cctgctctgg
    15721 atctttcacg cctgcctgaa cttctgtcgc gtattgacgg ttataaaggc cagcctgtca
    15781 cccgactggc agttatgctg aatttactgg tttttattcg ttccagtgaa ctcagatatg
    15841 cccgctggtc tgaaattgat attgacaatg ccatgtggac tattccagcc gaacgcgaac
    15901 ctctgccagg cgtaaaattt tcataccggg gctccaagat gcgaacacca catcttgtgc
    15961 cactcagcca acaggctgta gccatactgg cagaacttca gacatgggca ggtgaaaatg
    16021 gtctgatctt tacgggagca cacgacccac gtaaaccaat cagtgaaaat accgtaaata
    16081 aggccctcag agtgatgggg tatgacacaa ccaaggaagt ctgtggccat ggattccggg
    16141 cgatggcgtg cagtgcattg attgaatccg gcttatggtc aagagatgcg gttgaacgtc
    16201 agatgagtca tcaggagcgc aacggagtac gtgccgcgta tattcataaa gcagaacatc
    16261 tggaagaacg tcggcttatg ctgcaatggt gggcagattt tctggatgca aacagggagg
    16321 agtgtatcag tccgtttgaa tatgcaaaag ttaacaatcc actgaaaagg tgactgaacg
    16381 ccatttacac acatgctctt cctcttaagg ggaagagcac ccgcctcaat caatttcttt
    16441 tttatgccgg cggaaccatc agccaggctg aggttctcat tcaataactc aacgtcttac
    16501 cacagaacag aattcactgt attatcatgg gcaacgttaa tcccggacaa taaaaggaca
    16561 ccgatgccag gcatcacaat ttttatagcc tgcaggttat ggtccatcct tttttacagg
    16621 ttcagaatat gactggcaaa ttacgctttg aagtgaacga taaccagggc tgtttcatat
    16681 ttccggaaac ctggtttggc tcgttgctgg atgagtttga agaactgatt gatgcatacg
    16741 atgcggatga aatcagcgaa acaagttaca tcaacaaact caggcgcctt gcccgacagg
    16801 agaatgactt tattgatgta cacgctcact tggcatacgt atttctggag cagaatgccc
    16861 cgcgtaaggc gctgaatgct gcccttaaag ggctggcaat tggcaatcgt cttatccctg
    16921 aaggtttcag tggcagaatc atctggatac atcctgacaa ccgacctttt cttcgtgccc
    16981 tctatgcggc aatcctggca aatgcacatt tacagcgtca tcaggatgcc atcatgctca
    17041 ttgaaaaaat actggattac aatcccgaag ataatcatgg tgcacgctgg ctactgggac
    17101 cggaactgtt acgcactggc gctcatgaac aggcacgcca tatactgcag gaacatgcag
    17161 atgaattttc accttactgg tatgaactcg gccttctgca ttttcttaat ggggaactgg
    17221 taaaagctgc aactgcattt cgtcgcggct ttgcggcaaa tacttacatc gcagaaatac
    17281 tgtgcggaaa tctgcatcca tttccacttg ctgtatggca taacttttca ggcggtccgg
    17341 atactgccga agattattat gccacatacc acccgttatg ggggcaatat ccggaagcat
    17401 tgctgtttgt aaactggctc tataatcatt cctctgtgct gcatgaacgg gcagaaatca
    17461 ttaaatgcgc cgaaatgctg atgcaggaag atgatttcga aatatgcgaa agtattttaa
    17521 gacagcagga gaagttgcgt gaaagaattg atgagacgct ttctgagaaa attgtacaga
    17581 aatgcagaaa tatgaatggt gaatatgtct ggccctggat attgccgttt tcagcggcag
    17641 gcatgaaaca tactggcata cagtatcagt agatattgca ttagtgtatc ctgcacacaa
    17701 gtaataattt attcaccaat aataacactg ttaatgcccc cttcccctgg ttgtcagcca
    17761 ggggttatct tctgaatatt tcttttgaaa aggataacac agtaaattat ttttatgaat
    17821 tatcccatgg actcattaac accctttcat gatgctttat tgtcaaacac gttatggctg
    17881 acatcaaaaa aaaccggatt tcctctgcca gcgggtaatc accttcccgg tgttttcggt
    17941 tggtctggtt actcctgtct ggttattagc aagataattg ctataaacag tggaaaactc
    18001 atcgtacata atctggtgat gaacattacg cttattttcc cttgaccgga agaatcagag
    18061 gctgtggttt cagactgtct gccagtacat tcctctctcc gttaaaaacc ataacgggtt
    18121 cattatcttc gtctgtcagc agatt
//
LOCUS       DAYPHN010000096        17894 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000096 DAYPHN010000000
VERSION     DAYPHN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.096"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(174..1019)
                     /gene="sseA"
                     /locus_tag="ACG0BW_003063"
     CDS             complement(174..1019)
                     /gene="sseA"
                     /locus_tag="ACG0BW_003063"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFK7259993.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            1226..6187
                     /gene="yfhM"
                     /locus_tag="ACG0BW_003064"
     CDS             1226..6187
                     /gene="yfhM"
                     /locus_tag="ACG0BW_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFK7259994.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKIGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNERKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALQGYGDGEIQASISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLQGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAT
                     QLQALGIKGDSDEKRRASVDIGIFRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TVQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            6188..8500
                     /gene="pbpC"
                     /locus_tag="ACG0BW_003065"
     CDS             6188..8500
                     /gene="pbpC"
                     /locus_tag="ACG0BW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFK7259995.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLASGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKQFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLMDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            8649..9080
                     /gene="ndk"
                     /locus_tag="ACG0BW_003066"
     CDS             8649..9080
                     /gene="ndk"
                     /locus_tag="ACG0BW_003066"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFK7259996.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            9230..10384
                     /locus_tag="ACG0BW_003067"
     CDS             9230..10384
                     /locus_tag="ACG0BW_003067"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFK7259997.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRCYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            10669..11682
                     /gene="rodZ"
                     /locus_tag="ACG0BW_003068"
     CDS             10669..11682
                     /gene="rodZ"
                     /locus_tag="ACG0BW_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFK7259998.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATITPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            11709..12827
                     /gene="ispG"
                     /locus_tag="ACG0BW_003069"
                     /gene_synonym="gcpE"
     CDS             11709..12827
                     /gene="ispG"
                     /locus_tag="ACG0BW_003069"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFK7259999.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            12938..14212
                     /gene="hisS"
                     /locus_tag="ACG0BW_003070"
     CDS             12938..14212
                     /gene="hisS"
                     /locus_tag="ACG0BW_003070"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFK7260000.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPVLGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            14230..14850
                     /gene="yfgM"
                     /locus_tag="ACG0BW_003071"
     CDS             14230..14850
                     /gene="yfgM"
                     /locus_tag="ACG0BW_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFK7260001.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            14861..16039
                     /gene="bamB"
                     /locus_tag="ACG0BW_003072"
     CDS             14861..16039
                     /gene="bamB"
                     /locus_tag="ACG0BW_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFK7260002.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            16157..17629
                     /gene="der"
                     /locus_tag="ACG0BW_003073"
     CDS             16157..17629
                     /gene="der"
                     /locus_tag="ACG0BW_003073"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFK7260003.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            17692..>17894
                     /locus_tag="ACG0BW_003074"
     CDS             17692..>17894
                     /locus_tag="ACG0BW_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFK7260004.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVE"
BASE COUNT         4309 a         4405 c         5006 g         4174 t
ORIGIN      
        1 ccaaatgaat tcaaaatcta tcctttttta tctacttgac atcgttgatg cataaagcga
       61 gtattgagta gacgagatac acttgcagta atacaacctg ctggaaaggc cgcgaaccag
      121 accagcaata aaaaaccgcc aaattcggcg gttttttatt gcctgtaaaa agcttatttt
      181 acgggctcaa ccggtaaatc tgctcgtgcg ccccattcac tccatgcgcc gtcgtacagt
      241 ttcacgtttg gcacatcaag tgtcgcgagt gccaacaaaa ccacggctgc cgttacacca
      301 gagccgcagc tgacgataat cggtttgtca tagctgacgc cgcgaccaaa aaatatcgca
      361 tccagttcat cggtcgtttt tagttcgcct tcgcgcacca gttccgtcca cggaacgttc
      421 aatgcaccgg gaatatgtcc gcgacgtaaa cctgggcgag gttcatcaac ttctgcgtta
      481 aaacgtgcag ccgggcgggc atcgataatt tgcgccgtat tttcatggct tgccaataac
      541 acatcggtga ctttcaccac ggcttcagga ttaaacgcgg cgttaaactc tccttccggc
      601 agttctactg cgccttcttc taatagcaga tcatcacgct gccagcctgc aagtccacca
      661 cccagaatcg acactttctc tacaccaaag gtgcgcagca tccaccatgc tcgtggggct
      721 gagaaaagat taccttcgtc atagacaatc aggtgcttat cctggttaac gcctaattca
      781 cgcatcgcca cggcgaacgt ttccgggcgc ggcagcatgt gtggaagcgg ggaagtgtga
      841 tcagaaagcg cttctatatc aaaaaacact gcgccgggaa tatgtccatt cagatactcc
      901 tgagcaacgt tacgatcttc ctgtccaggc gacgccatgc gggcatcgat aatctgaatt
      961 tccgggtcat caatatgttc ggcgagccag tcggctccta caaaccatgt cgtggacata
     1021 ggcatctcca tttttgccgg ggttaggcta aattgtcgga tgtttagtga aaggcgacaa
     1081 gcaaaagagg ctgatttggc tgtctgagcc acaattctca ctaaaattcg gattgtatgg
     1141 gcgtgatact gaattacata acgtaaattc tcacgcataa taattattcg acgttgctaa
     1201 cgggccattg tggcagggat gaaaaatgaa aaagttacgc gtagccgcct gcatgctaat
     1261 gctggcgctg gcagggtgcg acaacaacga taacgcgcca acagcggtga aaaaagatgc
     1321 gccttctgaa gttactaaag cggcctcttc agaaaacgtg agttcagcaa aactttccgc
     1381 gccggagcga caaaaactgg cccaacagag tgccggtaag gcgctgacat tgctggatct
     1441 ctctgaagtt caacttgatg gcgcagccac gctggtgctg acgttctcca ttcctctcga
     1501 cccggatcag gatttctcac gcgttatcca tgtcgtcgat aagaaaatcg gcaaagtgga
     1561 tggtgcctgg gagctgtcag ataatcttaa agagttgcgt ttacgccacc tcgaaccgaa
     1621 acgtgatttg atcgttacta ttggcaagga agtcaaagca ctcaacaacg caaccttcag
     1681 taaagattat gaaaaaacta taactacccg cgacatccaa cccagcgtcg gttttgccag
     1741 ccgtggttcg ctgctgcctg gcaaagtcgt tgaagggctg ccggtaatgg cgctcaacgt
     1801 taataatgtc gatgttaact tcttccgcgt taagccagaa tctctgccag cattcattag
     1861 ccaatgggaa taccgcaatt cgctggcgaa ctggcagtca gacaaactgc tgcagatggc
     1921 ggatctggtc tacaccggac ggtttgatct caatcctgcg cgtaacaccc gtgaaaaatt
     1981 attgctgccg ctgggcgata tcaaaccgct tcagcaggcg ggcgtgtatc tggctgtgat
     2041 gaatcaggct ggacgttacg attacagtaa tcccgcgacg ctgtttacgt taagtgatat
     2101 cggcgtttca gctcaccgtt atcacaatcg tctggatatc tttacccaaa gtctggaaaa
     2161 cggcgcggcc cagcaaggaa ttgaagtctc tttattaaat gagaaagggc agactctgac
     2221 tcaggcaacc agtgacgctc aggggcatgt gcagctggaa aatgataaaa acgcggcatt
     2281 actgttggcg cgtaaagacg gtcagacaac gctactcgat ttaaaacttc cggcgctgga
     2341 cttagcagaa tttaacattg ctggcgcgcc aggctatagc aaacagtttt tcatgtttgg
     2401 cccacgcgat ctttatcgcc caggtgaaac ggtaatcctc aatggtttgc tgcgtgatgc
     2461 agacggtaaa gcgttgccca atcaacccat caagttagac gtgattaaac ccgatgggca
     2521 ggtactcagg agcgtcgtta gtcagccgga gaatggcctc taccacttta cctggccact
     2581 cgatagcaat gcggcaaccg gtatgtggca tattcgcgct aacacgggcg ataaccagta
     2641 tcggatgtgg gatttccacg tcgaagattt tatgccagag cgcatggcgc tgaatctgac
     2701 cggtgagaaa accccgctaa cgccgaaaga tgaagtgaaa ttctccgtgg tgggatacta
     2761 cctgtacggt gcgcctgcta atggtaatac tttgcaaggg caacttttcc tgcgcccact
     2821 gcgtgaagct gtgtcagcct tacctggttt tgaattcggc gatatagctg ccgaaaatct
     2881 ttcccgcacg ctggatgaag ttcagttgac gctggatgat aaagggcgcg gcgaagtttc
     2941 tacagaaagc cagtggaagg aaacgcattc cccattacag gttattttcc agggtagttt
     3001 gctggaatcg ggcggtcgcc cggtgacgcg ccgcgctgag caggctatct ggcctgccga
     3061 tgcattgccg gggatccgtc cgcagttcgc ctcgaaatcg gtttacgatt atcgtactga
     3121 cagcacggtg aaacagccca ttgttgatga aggcagtaac gccgcttttg acatcgttta
     3181 tagcgatgcg caaggcgtga aaaaagccgt gtcgggcttg caggtgcgcc tgattcgcga
     3241 acgccgcgat tactactgga actggtcaga agatgaaggc tggcagtcac agtttgatca
     3301 aaaagatctg atcgaaaatg aacaaactct ggatctgaaa gcggacgaaa ccggcaaggt
     3361 cagttttccg gtagagtggg gcgcttatcg tctggaagtc aaagcgccga atgaagcggt
     3421 cagtagtgtt cgtttctggg ctggctatag ctggcaggac aacagcgacg gtagcggcgc
     3481 agtgcgaccc gaccgtgtca cgctgaaact ggataaagcc agttatcgcc ctggcgacac
     3541 cattaagttg catatcgccg cgccaacggc gggtaaaggt tatgcgatgg tcgagtccag
     3601 tgaagggccg ctgtggtggc aagagattga tgttcgggct caagggctgg atctgacgat
     3661 tccggtcgat aaaacctgga atcgtcatga tctgtattta agtacgctgg tggtacgtcc
     3721 tggcgataaa tctcgctccg cgacgccaaa acgcgcggtt ggtgtgttgc atctgccgct
     3781 tggcgatgaa aaccgtcgcc tcgatctggc gctggaaaca ccagcaaaaa tgcgtcccaa
     3841 tcaaccatta accgtgaaaa ttaaagccag cactaaaaat ggcgagaagc ctaaacaggt
     3901 gaatgtgctg gtgtctgccg ttgatagtgg tgtgctgaat attactgact acgtcacgcc
     3961 agatccgtgg caggcgttct ttggtcagaa acgctatggc gcagacattt acgatattta
     4021 cggtcaggtt attgaaggtc aggggcgtct ggcagctctg cgtttcggtg gcgatggtga
     4081 tgagctgaaa cgtggtggta aaccgccggt caatcacgtc aatattgtcg cgcagcaggc
     4141 gctgccggta acgctcaacg aacagggtga aggctcggtt acactgccga ttggcgattt
     4201 taacggtgaa ttgcgcgtca tggcgcaagc ctggacggca gatgacttcg gtagcaacga
     4261 aaggaaagtg atagttgccg caccggtgat tgctgaactg aacatgccgc gttttatggc
     4321 gagtggcgat acctcgcgcc tgacgctgga tatcactaat cttaccgata aaccgcaaaa
     4381 actaaacgtt gccctgaccg ccagtggttt gcttgaactg gtcagcgatt cacccgcagc
     4441 cgttgaatta gcgccaggcg tacgtactac gctgtttatc ccggtgcgag cattgcaagg
     4501 ttatggcgat ggagaaattc aggcctccat tagtgggtta gcgttaccag gtgaaaccgt
     4561 tgccgaccag cataagcagt ggaaaatcgg cgtccgtcca gcgttcccgg cacaaacggt
     4621 taattacggt acggcgttgc agcctggtga aacctgggcg ctcccggcgg atggattgca
     4681 aaacttctcg cctgttacgc tgcaaggtca gctattgttg agcggcaaac caccgctgaa
     4741 tatcgcacgt tatatcaaag agttaaaagc gtatccttac ggctgtctcg agcaaaccac
     4801 cagcggcctg ttcccgtcac tttataccaa cgcaacccag ctacaggcgt tgggcatcaa
     4861 aggcgacagt gatgagaaac gccgtgcatc ggtcgatatc ggcattttcc gtttgctgca
     4921 aatgcaacgt gataacggtg gctttgcgct atgggataaa aacggtgacg aagagtactg
     4981 gctgacggct tacgtgatgg atttcctggt ccgcgcaggt gagcagggtt acagcgtgcc
     5041 gacagacgcc attaaccggg gaaatgagcg tctgctgcgc tatttacaag atccgggcat
     5101 gatgtcgatc ccgtacgcgg ataatctcaa agccagtaaa ttcgccgtac agtcttacgc
     5161 tgcgctggtg ttggcccgtc agcaaaaggc accactgggt gcgctgcgtg aaatctggga
     5221 gcatcgtgca gatgctgctt ctggattacc gctgctgcaa cttggcgttg cgctgaaaac
     5281 catgggtgat gcaacgcgtg gtgaagaagc gattgtgctg gcgctgaaaa cgccgcgtaa
     5341 tagtgatgag cggatatggc tgggtgatta cggtagtcca ctgcgcgaca acgcgttgat
     5401 gctctccttg ctggaagaaa ataaactgct acccgatgag caaaacactt tgctgaacac
     5461 gctttcgcag caggcgtttg gtgaacgctg gctatcaacc caagaaagta acgcgttgtt
     5521 cctggctgcc cgtacggttc aggatttacc gggtaaatgg caggcgcaaa cctctttctc
     5581 agctgagccg ctgacaggcg agaaagcgca aaacagtaat ctgaatagcg atcaacttgc
     5641 taccttacag gtgaccaaca gtggcgatca gccgttatgg ctgcgtgtgg atgccagcgg
     5701 ttatccgcaa tccgcacctt taccggcgaa caatgtgctg caaatcgagc gtcatattct
     5761 tggtactgat ggtaagagca aatcgctgga ctcgttacgt agcggcgatc tggtgctggt
     5821 gtggttgcag gtaaaagcca gcaacagcgt gccggatgcg ttagtcgttg atttactgcc
     5881 agcgggtctg gaactggaaa accagaacct ggcgaacggt agcgccagcc tggagcaaag
     5941 tggtggcgaa gtgcagaact tactgaacca gatgcagcag gcgagtatta agcatattga
     6001 gttccgtgac gatcgctttg tggcggcggt ggccgttgat gaataccaac cggtaacgct
     6061 ggtgtatctg gcgcgggcgg taacgccggg aacgtatcag gtaccgcaac cgatggtgga
     6121 atcaatgtat gttccccaat ggcgggcgac cggcgcggct gaagatttgc tgattgtcag
     6181 accgtaaatg cctcgcctgt taaccaaacg cggctgctgg ataacgctgg cagccgcgcc
     6241 ctttctcctt tttcttgcag cgtggggagc agataaactc tggcctctac cgctgcatga
     6301 agtcaatccc gcacgagtgg tcgtggcgca ggatggtacg ccgctctggc gtttcgccga
     6361 tgctgagggc atctggcgtt atccggtaac aatcgaagat gtttctccac gttaccttga
     6421 agcgctgatc aattatgaag atcgctggtt ctggaagcat ccgggggtga atccattctc
     6481 ggtggcgcgc gcagcatggc aagatcttgc ttcgggacgg gttatttccg gtggcagcac
     6541 gctcactatg caggttgctc gtttgcttga tcctcacccc aaaacatttg gcggcaaaat
     6601 tcgccagctc tggcgcgcgt tgcaactgga gtggcatctg tctaagcgtg aaattctgac
     6661 cttgtatcta aaccgcgctc cgtttggcgg tacgttgcag gggatcggtg cggcaagttg
     6721 ggcttatctc ggaaaatcgc ctgcgaattt aagctattcc gaggcggcaa tgctggcggt
     6781 gttgccgcaa gcgcccagcc gactacgccc ggatcgctgg ccggaacgtg ccgaagccgc
     6841 gcgtaataaa gttctcgaac ggatggccgt gcaaggtgtg tggtcccgtg agcaggtaaa
     6901 agagtcaagg gaagaaccca tctggctggc accccgacaa atgccgcaat tggcaccgct
     6961 gttttcgcgc atgatgctcg gtaaaagcaa aagcgacaaa atcgttacta cgctggatgc
     7021 cggtcttcaa cgacgtctgg aagaactggc gcaaaactgg aaaggacgat taccaccgcg
     7081 cagctcactg gcgatgatcg tggttgatca taccgatatg cgtgttcgcg gctgggtggg
     7141 ttcagttgat ctcaacgatg attcacgctt tggtcatgtt gatatggtca atgcgatccg
     7201 atcgccagga tcggtgctca aaccatttgt ttacggtctg gcgctggatg aaggattgat
     7261 ccacccggca tcactgctgc aagacgtccc ccggcgtacc ggtgattacc gacctggcaa
     7321 ctttgacagc ggttttcatg ggccgatcag catgagcgaa gcgctggtgc gctcgctgaa
     7381 tttaccagct gtgcaggtgc tggaagccta tggaccgaaa cagtttgcgg caaagttacg
     7441 caatgttgga ttgccgttat atttgcccaa cggcgctgcg ccgaatcttt cactcattct
     7501 cggcggcgct ggtgcaaaac tggaagatat ggcggcagcg tataccgcgt ttgctcgcca
     7561 tggcaaggca ggcaaattgc gcttacagcc tgacgatccg ctgcttgaac ggcctttaat
     7621 gtcgtcgggg gcggcgtgga tcattcgccg gattatggct gatgaagcgc aacccttgcc
     7681 ggatggtgcc ttgccgcgcg tcgccccact ggcatggaaa acgggcacca gctatggcta
     7741 tcgtgatgcc tgggcgattg gggttaacgc tcgctatgtc attgggatct ggactggcag
     7801 accggacggc acgcccgttg ttggtcagtt tggctttgcc agcgccgtac cattgttgaa
     7861 tcaggttaat aatatcttac tgtcgcgcag tgcgaatctg ccggaagacc cacgtcctga
     7921 ctctgtaagt cgtggcgtta tctgctggcc gggcggacag tctttgccgg aaggtgacgg
     7981 taactgtcgc cgccgcctgg caacctggct gctggacggg agtcagccgc caactctatt
     8041 actgccggag caggaaggca ttaatggcat tcgtttcccc atctggctgg atgaaaacgg
     8101 caaacgtgtt gccgctgatt gccctcaggc gcgacaagaa atgataaatg tctggccgct
     8161 accgctggaa ccctggttgc ccgcatcaga acgccgcgct gtgcgcttac caccggcctc
     8221 gacaatttgc ccgccatacg gtcacgatgc acaactccca ctgcagctga caggtgttcg
     8281 cgatggcgcg attattaaac gtttaccggg cgcagcggaa gcgactttgc cgttgcaatc
     8341 aagtggaggg gcaggtgaac gctggtggtt tctgaatggc gaaccgttaa ctgaacgcgg
     8401 gcgcaacgtg accctgcatt tgatggataa aggcgattat caattgctgg tgatggatga
     8461 ggtggggcaa atcgcgacag tgaaatttgt catgcaatag tctgttttgt ctctgattgt
     8521 tgcttaaact aggtttattt taaaaaaatg ttactttcat caatagtcaa cggccctgtt
     8581 gctcattata atccgcgcca tctcgtacgc tggtacagac aacaacagaa caatttacag
     8641 aggtaaaaat ggctattgaa cgtacttttt ccatcatcaa accgaacgcg gtagcaaaaa
     8701 acgtcatagg taatatcttt gcgcgctttg aagctgcagg gttcaaaatt gtcggcacca
     8761 aaatgctgca cctgaccgtt gaacaggctc gtggctttta tgctgaacac gatggaaaac
     8821 cgttctttga tggtctggtt gaattcatga cctctggccc gatcgtggtt tccgtgctgg
     8881 agggtgaaaa cgccgttcag cgtcaccgcg atctgctggg cgcgaccaat ccggcaaacg
     8941 cactggccgg tactctgcgt gctgattacg ctgacagcct gaccgaaaat ggtacccacg
     9001 gttctgattc cgtcgaatct gccgctcgcg aaatcgctta tttcttcggc gaaggcgaag
     9061 tgtgcccgcg cacccgttaa taatttcgta aatgccgcgt gcaaacgtgg catccgtgcg
     9121 ccagaatttg tacaatgcag cgcccccgga cgagcagccg ctcaccgggg cgtttctttt
     9181 ttcaaccctc caggggccat aacgtgtaat aacgaggccg gaatagcata tgtctgaaca
     9241 attagtcaca cctgaaaacg tcaccacgaa agatggaaaa atcaacctgc tggatctcaa
     9301 ccgtcagcag atgcgggagt tttttaaaga tttaggtgaa aaacccttcc gcgccgatca
     9361 ggtgatgaag tggatgtatc actattgctg cgacaacttt gatgagatga ccgacatcaa
     9421 taaagtgttg cgcggcaaac tgaaagaggt ggcggaaatc cgtgcaccgg aagtggttga
     9481 agaacagcgt tcatctgacg gcaccattaa atgggcgatc gccgttggcg atcagcgcgt
     9541 tgaaacggtg tatatcccgg aagacgaccg tgccacgctc tgcgtctctt cgcaggtggg
     9601 gtgtgcgctg gagtgtaaat tctgttccac cgcccagcag ggctttaacc gcaacctgcg
     9661 ggtatcggaa attatcggcc aggtgtggcg tgcggcgaaa attgtcggcg cagcaaaagt
     9721 caccggtcag cgcccaatca ctaacgtagt gatgatgggt atgggcgagc cgctgctcaa
     9781 cctgaacaat gtcgttccgg cgatggaaat catgcttgat gatttcggtt ttggcctgtc
     9841 taaacgtcgc gtgacgcttt ccacttccgg tgtagttccg gcgctggata aactgggcga
     9901 tatgatcgac gttgcactgg caatttccct gcacgcgccg aacgacgaaa ttcgtgacga
     9961 aattgtgccg atcaacaaaa agtacaatat cgaaacgttc cttgctgcgg tgcgctgtta
    10021 tctggagaaa tccaacgcca atcagggccg agtcactatt gaatacgtga tgcttgacca
    10081 cgttaacgac ggcactgaac acgcgcacca actggcggag ctactgaaag atacgccgtg
    10141 taagatcaac ctgatcccgt ggaacccgtt ccctggcgca ccgtatggac gcagttcgaa
    10201 cagccgtatc gaccgtttct caaaagtgct gatgagctac ggtttcacca ctattgtgcg
    10261 taaaactcgt ggtgatgata tcgatgctgc ctgtggtcag ttggcgggcg atgttatcga
    10321 ccgtacgaaa cgtaccctgc gtaaacggat gcagggtgaa gccatcgata ttaaagcggt
    10381 ctgatagcca ttacgccacg gttactttat gtaccgtggc gtaatctttt atgaatgaag
    10441 tattaagttg caattgtctg gtcaatatta acggtgcgtt tttgctgact ttaaggcagt
    10501 atgtaacgat gcaacagtaa gttagcctta gtgaatgtgg gctttgtcac gagcacacag
    10561 acggtcttat actgtatgat aacggttaac ttaacggatg tttcgcggtg tgggtgagca
    10621 tgatggttca ccggcatctc aattctcatt taaacgtacc tgcagcgaat gaatactgaa
    10681 gccacgcacg accaaaatga agcacttact accggcgctc gcctgcgtaa tgctcgcgaa
    10741 caactaggac ttagtcagca ggccgttgcc gagcgacttt gcctgaaggt ttccacggta
    10801 cgcgacattg aagaagataa ggcacccgcc gatcttgctt caacattcct gcgcggatat
    10861 atccgctctt atgcgcgtct ggtacatatt ccagaagaag aactgctgcc agggctggaa
    10921 aagcaggctc cactacgggc tgcaaaagtt gcgccgatgc agagtttttc cctcggtaaa
    10981 cgccgcaaaa aacgcgacgg ctggctgatg accttcacct ggctggtgtt gtttgtggtt
    11041 atcggcctga gcggtgcctg gtggtggcaa gaccacaaag ctcagcagga agagatcacc
    11101 actatggccg atcaatcttc ggcggaactg agcagtaata gcgagcaggg gcagagtgtt
    11161 ccgttaaata cgtcgacaac tacagacccg gctacaacgt ccacgccgcc agcgtctgtg
    11221 gatactaccg caaccaacac gcaaacacct gccgtaactg cgccagcacc agctgttgat
    11281 ccgcaacaga atgcggttgt ttcgccttca caggcaaatg ttgatactgc tgcgaccccg
    11341 gcaccaacgg caacaataac gccagatggt gctgcgccgt tgccaaccga tcaggcaggc
    11401 gtgactacgc cggcggctga tccgaatgcg ctggtgatga actttactgc cgattgctgg
    11461 ctggaggtca ctgatgctac cggtaaaaaa ctgtttagcg ggatgcagcg taaagacggt
    11521 aatttgaatt taaccggcca ggcaccgtat aaactgaaaa ttggtgcgcc agccgcagta
    11581 cagatccagt atcaagggaa acctgtcgat ctgagtcgtt ttatcagaac taaccaggtt
    11641 gcgcgtctga ccctcaatgc cgaacaatca ccggcgcagt aacagacggg taacgcggga
    11701 gatttttcat gcataaccag gctccaattc aacgtagaaa atcaacacgt atttacgttg
    11761 ggaatgtgcc gattggcgat ggtgctccca tcgccgtaca gtccatgacc aatacgcgta
    11821 cgacagacgt cgaagcaacg gtcaatcaaa tcaaggcgct ggaacgcgtt ggcgctgata
    11881 tcgtccgtgt ctccgtaccg acgatggacg cggcagaagc gttcaaactc atcaaacagc
    11941 gggttaacgt gccgctggtg gctgacatcc acttcgacta tcgcattgcg ctgaaagtag
    12001 cggaatacgg cgtcgattgt ctgcgtatta accctggcaa tatcggtaat gaagagcgta
    12061 ttcgcatggt ggttgactgt gcgcgcgata aaaacattcc gatccgtatt ggcgttaacg
    12121 ccggatcgct ggaaaaagat ctgcaagaaa agtatggcga accgacgccg caggcgttgc
    12181 tggaatccgc catgcgtcat gttgatcatc tcgatcgcct gaacttcgat cagttcaaag
    12241 tcagcgtgaa agcgtcagac gtcttcctcg ctgttgagtc ttatcgtttg ctggcaaaac
    12301 agatcgatca gccgctgcat ctgggtatca cagaagccgg tggtgcgcgc agcggggcgg
    12361 ttaaatccgc cattggttta ggtctgctgc tgtctgaagg cattggcgac acgctgcgcg
    12421 tatcgctggc ggccgatccg gttgaagaga tcaaagtcgg tttcgatatt ttgaaatcgc
    12481 tacgtatccg ttcgcgcggg atcaacttca ttgcctgccc gacctgttcg cgtcaggagt
    12541 ttgatgttat cggtacggtt aacgcgttgg agcaacgcct ggaagatatc atcactccaa
    12601 tggacgtttc gattatcggc tgcgtggtga atggcccagg tgaggcgctg gtttctacac
    12661 tcggcgtcac cggcggtaac aagaaaagcg gcctctatga agatggcgta cgcaaagacc
    12721 gtctggacaa caacgatatg atcgaccagc tggaagcgcg cattcgtgcg aaagccagtc
    12781 agctggacga agcgcgtcga attgacgttc agcaggttga aaaataataa cgtgatggga
    12841 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
    12901 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
    12961 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
    13021 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
    13081 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
    13141 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
    13201 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
    13261 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
    13321 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaattga ttatgctcac
    13381 cgcccgctgg tggcgcgcgc tgggtatctc cgagcacgta actcttgagc tgaactctat
    13441 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
    13501 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
    13561 ggattcaaaa aatccggaag tgcaggcgct tctcaacgat gctccggtat taggcgatta
    13621 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgcttg agagcgcggg
    13681 gatcgcttat accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
    13741 tttcgagtgg gtgactaaca gcctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
    13801 tgacggtctg gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
    13861 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
    13921 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
    13981 tgagcgtctg cgtgatgaat taccgggcgt gaaattgatg accaaccacg gcggcggcaa
    14041 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
    14101 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
    14161 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
    14221 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
    14281 tttgctgaaa atggcaaagc actggctgtt ggggtgattt tgggcgttgg cgcgctaatt
    14341 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
    14401 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
    14461 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
    14521 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
    14581 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
    14641 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
    14701 gccattgttg ccgacctgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
    14761 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
    14821 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
    14881 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
    14941 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
    15001 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
    15061 ggcggacaac gttgtctatg cagcggaccg cgctggttta gtaaaagcgc tgaatgcgga
    15121 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
    15181 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
    15241 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
    15301 cgcgggtgaa gcactttcgc gtccggtggt cagcgacggt ctggtgttaa tccacaccag
    15361 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
    15421 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
    15481 cgtcgtgggt ggtgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
    15541 ttggcagcag cgaatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
    15601 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
    15661 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
    15721 tgatttcatc gtcgacggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
    15781 gttgaccatt gatggcggcg ttacgctgtg gacacaaagc gatctgctgc atcgcctgct
    15841 gacttctccg gtgctgtata atggcaacct ggtggtaggt gacagcgaag gttatctgca
    15901 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
    15961 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
    16021 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
    16081 gccgttttcc tgtttttaac aacgacacga atatagcgtg cgttgtctga tgatttataa
    16141 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
    16201 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
    16261 tctgactcgt gaccgtaagt acggtcgtgc agaaattgaa ggccgtgagt ttatctgcat
    16321 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
    16381 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
    16441 gatgccggcg gatgaagcga ttgctaaaca tctgcgctcc cgtgaaaaac cgaccttcct
    16501 ggtggcaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
    16561 tggtttaggg gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
    16621 ggagcacgtg ctgctgccgt ggatggaaga tctcgcaccg caagaagaag tcgacgaaga
    16681 cgctgaatac tgggcgcaat ttgaagcaga agaaaacggc gaagaagaag aggaagatga
    16741 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
    16801 gtctacactc actaaccgta ttcttggtga agagcgcgtt gttgtttacg acatgcctgg
    16861 cacgacgcgt gacagtatct acattccaat ggaacgcgac ggacgtgagt atgtgctcat
    16921 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctcggt
    16981 aatcaaaacg ttgcaggcca ttgaagatgc caacgtggtg atgttggtga ttgacgcccg
    17041 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
    17101 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
    17161 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
    17221 cttgcacggc agtggtgttg gtaacttgtt tgaatcagtg cgtgaagcgt atgacagctc
    17281 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    17341 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ccggtggtta
    17401 taacccgccg attgtggtga ttcacggtaa ccaggtgaaa gacctgcctg attcctacaa
    17461 gcgttatttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    17521 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    17581 gatgcgtaaa cgtaagcgcc tgatgaagca tatcaagaaa agtaagtaat cacttatctg
    17641 gtttcccttt ccacacggag agggaaaccg aataacacgc gaggagtatt tatggcactc
    17701 aactgtccaa cctgcaaaac ccctctcgaa cgcaatggcg agactgccca ctgtgcaact
    17761 tgcgacaaag actttaccgt ccaggcactt tgccctgagt gtcatcagcc gttacaagtg
    17821 ttaaaagctt gtggtgctgt ggattatttc tgccaacatg ggcacggttt aatatctaaa
    17881 aaacgcgttg aatt
//
LOCUS       DAYPHN010000097        17704 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000097 DAYPHN010000000
VERSION     DAYPHN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.097"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(124..945)
                     /gene="cysE"
                     /locus_tag="ACG0BW_003075"
     CDS             complement(124..945)
                     /gene="cysE"
                     /locus_tag="ACG0BW_003075"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFK7260005.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1025..2044)
                     /gene="gpsA"
                     /locus_tag="ACG0BW_003076"
     CDS             complement(1025..2044)
                     /gene="gpsA"
                     /locus_tag="ACG0BW_003076"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFK7260006.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDHIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2044..2511)
                     /gene="secB"
                     /locus_tag="ACG0BW_003077"
     CDS             complement(2044..2511)
                     /gene="secB"
                     /locus_tag="ACG0BW_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFK7260007.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(2574..2825)
                     /gene="grxC"
                     /locus_tag="ACG0BW_003078"
     CDS             complement(2574..2825)
                     /gene="grxC"
                     /locus_tag="ACG0BW_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFK7260008.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDANAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(2968..3399)
                     /locus_tag="ACG0BW_003079"
     CDS             complement(2968..3399)
                     /locus_tag="ACG0BW_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFK7260009.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            3644..5188
                     /gene="gpmM"
                     /locus_tag="ACG0BW_003080"
                     /gene_synonym="pgmI"
     CDS             3644..5188
                     /gene="gpmM"
                     /locus_tag="ACG0BW_003080"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFK7260010.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5222..6481
                     /gene="envC"
                     /locus_tag="ACG0BW_003081"
     CDS             5222..6481
                     /gene="envC"
                     /locus_tag="ACG0BW_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFK7260011.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRSSLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            6485..7444
                     /locus_tag="ACG0BW_003082"
     CDS             6485..7444
                     /locus_tag="ACG0BW_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFK7260012.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(7431..8465)
                     /gene="waaH"
                     /locus_tag="ACG0BW_003083"
     CDS             complement(7431..8465)
                     /gene="waaH"
                     /locus_tag="ACG0BW_003083"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFK7260013.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(8704..9729)
                     /gene="tdh"
                     /locus_tag="ACG0BW_003084"
     CDS             complement(8704..9729)
                     /gene="tdh"
                     /locus_tag="ACG0BW_003084"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFK7260014.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGVTRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(9739..10935)
                     /gene="kbl"
                     /locus_tag="ACG0BW_003085"
     CDS             complement(9739..10935)
                     /gene="kbl"
                     /locus_tag="ACG0BW_003085"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFK7260015.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(11211..12068)
                     /gene="yibB"
                     /locus_tag="ACG0BW_003086"
     CDS             complement(11211..12068)
                     /gene="yibB"
                     /locus_tag="ACG0BW_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFK7260016.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKSRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            12372..13304
                     /gene="rfaD"
                     /locus_tag="ACG0BW_003087"
     CDS             12372..13304
                     /gene="rfaD"
                     /locus_tag="ACG0BW_003087"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFK7260017.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            13314..14360
                     /gene="rfaF"
                     /locus_tag="ACG0BW_003088"
     CDS             13314..14360
                     /gene="rfaF"
                     /locus_tag="ACG0BW_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFK7260018.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     ALEELNALLLQEEA"
     gene            14364..15356
                     /gene="rfaC"
                     /locus_tag="ACG0BW_003089"
     CDS             14364..15356
                     /gene="rfaC"
                     /locus_tag="ACG0BW_003089"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFK7260019.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRDLIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            15353..16561
                     /gene="rfaL"
                     /locus_tag="ACG0BW_003090"
     CDS             15353..16561
                     /gene="rfaL"
                     /locus_tag="ACG0BW_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFK7260020.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTCLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELIAYYKDYQQGVMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFAVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTANLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVVDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     ISLMMLYCAILKECIKNGVQNKNRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(16599..>17704)
                     /locus_tag="ACG0BW_003091"
     CDS             complement(16599..>17704)
                     /locus_tag="ACG0BW_003091"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFK7260021.1"
                     /translation="SIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFVNDN
                     CSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESIIVVHNSMKLYTQI
                     RKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLE
                     TYQSTFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAVAHKNLKLVVIG
                     DYTEMSKSDKGAYQHNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEP
                     FCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPEL
                     TAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         4674 a         4408 c         4234 g         4388 t
ORIGIN      
        1 tgatttacag cggcaagttt tacttcaaat gaatgtttca taaactgcac cttcagatgt
       61 cagattatgt ccaacatttg gggtgcagtt caattacatc gcatccggca caatcacagg
      121 acgtcagatc ccatccccat attcaaacgt atggttaata ccgttgaaat gctgatccat
      181 atccattgac ggtttatcgc tgtctggttt accgacaatg cgtgccggaa cgccagcggc
      241 ggtggtatgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg
      301 cccaacttca atattgccga gaattttcgc gcccgcgcca atcatcacac cttcacgaat
      361 tttcggatga cggtcgccac cagatttacc cgtaccgcca agcgtcacag attgcagaat
      421 cgatacgtcg ttttcaatca ccgccgtttc acccacaacg atgcccgtgg cgtggtcaag
      481 catgataccg cggccaattt ttgctgccgg gtgaatatca acctggaacg ttacagaaac
      541 ctggttttgc agaaatattg ccagtgcgcg gcggccctga ttccacagcc agtgaccgat
      601 acgataggcc tgcaaggcat gaaaaccttt cagatataac aagggggttg agtatttatc
      661 caccgccggg tcgcgggtac gcactgcctg aatatcacag gccgcagagg cgatcatttc
      721 cgggtcggcg gcgtaggctt cttccaccac ttcacggata gcaatagcag gcataattgg
      781 cgatgacagc ttgttcgcca gcatgtagct cagtgcactg cccaggtttt cgtgcttgag
      841 tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc
      901 agctttaata ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg
      961 atgagataat gaccggcccg ttctgcgcgg gccgggtcat agaggtaaca aaggttccct
     1021 tggtttagtg gctgctgcgc tcgtccttgc gtgcacgacc taataacgtc aatgctgcct
     1081 cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcaa
     1141 cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccacct
     1201 gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc atgccaaaac
     1261 ggcggttacg cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc
     1321 ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag acgcgacatc tcagccagcc
     1381 cacgagtgat cagcgccgta cgcgcattcg caccaaaacc gataccgtcg gacattcccg
     1441 cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg cacaccgatg aaatccggat
     1501 tgctgtaaac gcggaaactt ttgccgcagt gcagcagttg ctggagatca tcagcaaagg
     1561 tctggtcggt agaggccagc gaaatagccg tcggtaaacc tgctgccagt tctttcgcaa
     1621 acgttgggcc agagataacc gccagcggaa tgtgatcgcc taacgcttca cgcgccacgt
     1681 cctgtaacag acgcccggtt tccgcttcca gacctttggt cgcccacacc agccgcgcat
     1741 caggacgcat cagcggttta atctgacgca gcacttcacc aaagacatgg ctgggtacga
     1801 cgacgagaat attacggctg gctgccagcg cagtggcgag atcgctttca agatggagcg
     1861 tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg
     1921 cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtga
     1981 tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt
     2041 tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat
     2101 gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac cgcgggatac
     2161 catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcacccag
     2221 gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac
     2281 ttcacacagg aacgcggttt cttcgcccag ggaggccgtt acggtaacac gcagtaccac
     2341 ttcgtaaacg tcatctgcca gttgagaaga tgccgtatcc agatcaagtt taacttctgg
     2401 ctgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaaa tatccttggt
     2461 ataaatacgt tggatctgga aagtcatttc ggtgttgttt tgttctgaca tgtgtagaaa
     2521 acccttaagt gttgtcctta aatactgcgc aatgtaatgc caacgttcac acgttatttc
     2581 agcaggggat ccagtccacc acgtgcatcc aatgcataca agtcatcaca gccgccaatg
     2641 tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac cgctgcgttt gatcatctct
     2701 tcacgcttag cggcgttggc atcgatcggt aactcctgaa aactcacgcc cttgctgctc
     2761 agcagtgctt tcgcacgatg gcaatacggg caggtttctt tggtatagat ttcaacattg
     2821 gccataactt agctcctgtt tttttaccct gaaaatttct tgttgcaggg cggctgcaaa
     2881 atggagtgtc cccagaagct tataacaata agtcgctggg ggcctaattg cagctaacgg
     2941 cctgcatcat gaaagacgac aggtaaatta tttgccgcgc accagaggta agttttcgcc
     3001 agcccagcca gcaacgccct ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag
     3061 cgcgtttgca ggctcctggc actgcatgcc agaaccgtct accacgataa ccggtttgtc
     3121 tttgtgcttc tcaagctcac caacattgtt ggctttgatt tcgctcggca acaggttgat
     3181 agaacctgcg atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc
     3241 atcttctttg ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt
     3301 caggctctta aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag
     3361 tatgggatga cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg
     3421 tagtgattaa aaaaaccaag aaaggagtat acctgcgcgg tgcggcaaat acagccagcg
     3481 cgttaactgg aatgcaattt tgcggggcgc gacgaaaaaa aagcgcaaat ctggcatcgg
     3541 cttgctgcga gcggtcgtat attttgatct tcagaggcta ttttatcgat tcagctgtag
     3601 taaaattacg caaattttga ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa
     3661 acctatggta ctggtgattc tggatggcta tggctatcgt gaagaacagc aggataacgc
     3721 catttttagt gctaaaaccc cggtaatgga tgcactgtgg gccaatcgtc cgcataccct
     3781 gatcgacgct tccggtctgg aagtcggtct gcctgaccgt cagatgggta actccgaagt
     3841 aggccacgtt aacctgggtg ccggccgcat cgtgtatcag gacctgactc gtctggacgt
     3901 tgaaatcaaa gatcgcgctt tcttcgctaa cccggtactg actggcgcgg tagataaagc
     3961 gaaaaacgca ggcaaagcag ttcacattat gggtctgctg tccgcaggcg gcgtacacag
     4021 ccacgaagat cacatcatgg cgatggtaga actggccgct gaacgcggcg cagaaaaaat
     4081 ctacctgcac gcattccttg acggtcgcga cactccaccg cgcagtgccg aatcctcgct
     4141 gaaaaaattc gaagaaaaat ttgccgcact gggcaaaggc cgcgtagcgt ccatcattgg
     4201 tcgttactac gccatggacc gcgataaccg ttgggatcgc gttgaaaaag cttatgacct
     4261 gctaactctg gcgcagggtg agttccaggc cgataccgcc gttgctggtt tgcaggctgc
     4321 ttatgctcgc gacgaaaacg acgaattcgt gaaagcgacc gttatccgtg cagaaggtca
     4381 gccagacgcg gcaatggaag acggcgatgc gctgattttc atgaacttcc gtgctgaccg
     4441 cgcacgtgaa atcactcgtg cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa
     4501 agtggttaac gtcgatttcg tgatgctgac cgaatacgcc gctgacatca aaactgcggt
     4561 tgcttaccca cccgcttccc tggttaacac cttcggcgag tggatggcga aaaacgacaa
     4621 aactcagtta cgtatttccg aaaccgaaaa atatgcccac gttactttct tcttcaacgg
     4681 tggcgtagaa gagtcgttca aaggcgaaga tcgcattctg atcaactcac cgaaagtggc
     4741 tacctacgat ctgcaaccgg aaatgagctc cgcagagctg accgaaaaac tggttgcggc
     4801 aatcaagagc ggcaaatacg acaccattat ctgtaactat ccgaacggcg acatggtagg
     4861 tcacaccggg gtgatggaag cggcggttaa agcggttgaa gcgctggatc actgcgtgga
     4921 agaagtcgcg aaagcagttg agtccgtggg tggacaactg ctgatcaccg ctgaccacgg
     4981 taacgctgaa cagatgcgcg atccggcaac gggtcaggca cacacggcac acaccaacct
     5041 gccagttccg ctgatttatg ttggtgataa gaacgtgaaa gcggttgaag gcggcaaact
     5101 ttctgacatc gcgccgacca tgttgtcgct gatgggtatg gaaatcccgc aagagatgac
     5161 tggtaagccg ctgttcatcg tggaataatc cctccccatg aggggaaagg cgattaatac
     5221 catgacacgg gccgtgaaac cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt
     5281 tcttagcgct ggcgtattgt tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact
     5341 caaatctatt caggccgata tcgccgcgaa agagcgcgcg gtacgccaaa agcaacaaca
     5401 acgctccagc ctgcttgctc agttgaaaaa gcaggaagaa gcgatctcgg aagccacccg
     5461 taagctgcgc gaaacgcaaa acacgctcaa tcaactgaat aaacagattg atgagatgaa
     5521 cgcgtcgatt gccaaactgg agcagcaaaa agccgcccag gagcgcagcc tcgccgcaca
     5581 actggatgcc gcattccgtc agggcgagca taccggtatt cagctgattc tcagcggtga
     5641 agaaagccag cgtggacagc gtttacaggc ttatttcggc tatctcaacc aggcgcgaca
     5701 agaaaccatt gctcagttga aacaaacgcg tgaagaagtc gctatgcaac gcgccgagct
     5761 ggaagagaaa cagagcgagc aacaaacgct gttgtatgag cagcgcgccc aacaggcgaa
     5821 gctgacccag gcgctgaacg agcgtaaaaa gacgctggca gggctggagt cttccatcca
     5881 gcaaggtcag caacagttga gcgagctgcg cgccaacgaa tcccgtctgc gtaacagcat
     5941 tgcccgtgcg gaagccgcgg cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc
     6001 ggttcgcgac cgccagaaag aagcgacgcg caaaggcacc acctacaaac cgaccgaaag
     6061 cgaaaaatcg ctgatgtccc gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg
     6121 gccagttcgc gggccgacgc tgcatcgcta tggcgagcag ctacagggtg aattacgctg
     6181 gaaaggaatg gttatcggtg cctctgaagg tactgaagtt aaagcaattg ccgacggtcg
     6241 ggtgattctg gctgactggc tgcaaggcta cggtctggtg gtggtggttg agcatggtaa
     6301 aggcgacatg agtctttacg gctataatca gagcgcactg gtgagcgttg gttcgcaggt
     6361 tcgcgcgggc cagccgattg cactggtggg cagcagtggc ggtcagggtc ggccttcact
     6421 ctatttcgaa attcgccgcc agggtcaggc ggtcaatcca cagccgtggt tgggaagata
     6481 agttttgttt ccatttcgtc gtaacgttct tgcatttgcc gctctgttgg cgctctcctc
     6541 ccccgtaatt gctggcaagc ttgccatcgt cattgatgat tttgggtatc gcccgcacaa
     6601 cgaaaaccag gtgctggcga tgccttccgc tatatccgtc gctgtattac ccgattcacc
     6661 gcacgccaga gagatggcga ccaaagcgca taacagcggt cacgaagtgt tgattcatct
     6721 accgatggca ccactgagta aacagccgct ggagaaaaat acgctacgcc cggagatgag
     6781 cagcgacgaa attgagcgca ttattcgtag tgcggtcaat aacgtgccct atgccgtggg
     6841 gatcaacaac cacatgggca gcaagatgac ctctaacctg tttggtatgc agaaagtgat
     6901 gcaggcgctg gagcgttaca atctttactt cctcgacagc gtaaccatcg gtaataccca
     6961 ggcgatgcgc gcagcgcaag gcactggcgt gaaggtgatc aaacggaaag tgttcctcga
     7021 cgattcgcaa aatgaagcgg acatccgtgt gcaattcaat cgcgcaattg acctggcacg
     7081 tcgcaacggt tcgaccattg ccattgggca tcctcaccct tccacggtac gcgtgttaca
     7141 acagatggtt tataacctgc cgccagacat tacgctggtg aaagccagca gcttgctgaa
     7201 tgaaccgcag gttgatacgt ctacaccgcc gaaaaacact gtgcccgaca caccgcgtaa
     7261 tccgttccgt ggcgtgaagc tctgcaaacc gaagaaaccg ctggaacctg tttatgctaa
     7321 tcgcttcttt gaagtattaa gcgaaagcat cagccagagc acgctgatcg tttacttcca
     7381 gcatcagtgg caaggctggg gcaaacagcc tgaagcggcg aagcttaacg ctagcgtaaa
     7441 ttaaaggcgc tacgcgtaat gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag
     7501 cggaaagacc acagtaacgc ctgataaccc accttcacgc tgcgcacatt ggtaatcagg
     7561 cgcttataca taccggaagt aaatatctca gcaatcatcc gttggcgggt aataatatcc
     7621 ggctctttgc gcaccgcatg gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat
     7681 tccggataaa tcgtaatttt gtcggcataa tttcgattta atttctctag caggcgggta
     7741 atcttaatat agtgacgttg ataattaagg tttttattcc cttgtctatt caaccgactc
     7801 actgacgtat tatgcagata atatttatat aacgattgct cggtatatcg cgcacgcagc
     7861 gcattaaaca taaattctgt tgtccagaca atatcctgat gatgtaatcc ggcaataaat
     7921 ttaatgttat ttttaacaat aacatcgcgg cgataaaccc ccatccagac aacgtgagtc
     7981 caacggcggg aagaaagccc catccgcaac caatccgggc cggttaatac gccggttgag
     8041 cgaaggcgat cggtggggat ggattgccag gtttctcccg tttcacgaaa acaccagtca
     8101 gcgttgcact gcgccacgtc gaggtcgtcc tctaacgcca tggtcatcag cgtttcgtac
     8161 atggtgggat acacctcatc atcagcatcg acaaaagcga catatttgcc cgtcgccacc
     8221 tcgatcccac gattacgcgc caccgatgcg ccagcattcg cctgatgcaa caaacgaacg
     8281 tgcggatagt tttctgcgta atgctttgct atttcaacag aattatccgt tgaaccatcg
     8341 ttaataataa tgatttccag agcagtccag gtttgcgtaa ttaaagattc catacaagtg
     8401 cggaaatcat cacccgcatt atataacggg ataataacac taagtttatt ggtgctgttc
     8461 atcataaatt accgacaacc tctgaagtta tcctgacgtt ttacaggctt tgtattaaga
     8521 aactattaag cctgtgagat attattttta caggtgagag agtcacttca gggtagagag
     8581 ataatgagaa cagctatata agagatcagc gcaattacga actctttatt acactgacag
     8641 gattttacat atttaacgga ttatcctcag gaaaaagggc tggaatacca gcccttgttt
     8701 gtgttaatcc cagctcagga taactttccc ggactggccc gaacgcatgg cgtcaaagcc
     8761 cttctggaaa tcatcgatag agaaacgatg ggtaatgatc ggcgagagat cgaggccaga
     8821 ctgaatcagc gccgccatct tgtaccaggt ttcaaacatc tcacgaccgt aaataccttt
     8881 aatgaacaag cccttaaaga tcactttggt ccagtcgata gacatatcag atggtggaat
     8941 acccagcatc gcaatacgac cgccgtggtt catggtgtcg agcatggtac gaaacgctgg
     9001 cggcgcaccg gacatttcca gaccgacatc aaagccttcg gtcatgccca gttctgccat
     9061 cacatcattg aggttctctt tagcgacgtt aaccgcacgg gtgacaccca ttttacgcgc
     9121 caactcaagg cggtattcgt taacatcagt aatcaccacg ttacgtgcac caacgtgttt
     9181 cgccaccgcc gccgccataa taccaatcgg gcctgcacca gagaccagca catcctcgcc
     9241 caccagatca aacgacagcg cggtatgcac ggcgttaccg aagggatcaa aaatggaagc
     9301 cagatcatcg gagatattgt cagggatttt gaaagcgttg aacgccggaa tcaccagata
     9361 ctcggcgaag cagcccggac ggttaacgcc gacgccgatc gtgttgcggc acagatgggt
     9421 acgaccgcca cgacagttgc gacagtgacc gcaggtgata tggccttcgc cagaaacgcg
     9481 atcgccaatc ttgaagcctt tcacttcctg accaatacct accacttcac cgacatattc
     9541 atggcccacg accatcggca ccgggatggt tttttgcgac cactcatccc agttatagat
     9601 gtgaacgtca gtcccgcaga tggctgtttt acggatttta atcagcagat cgttatgccc
     9661 gagttccggt acaggaacgt cggtcatcca gatgccctct tccgctttca gtttggataa
     9721 cgctttcatc tcacatcctc aggcgataac gcccagttgt ttaccaatac gcgtgaatgc
     9781 ttctactgca cgcgtaattt gctcaggggt atgcgccgca gacatctggg tacgaatacg
     9841 cgcctgacct ttcggaacga ccggatagaa gaaaccggtg acgtaaatgc cctctttttg
     9901 cagctcacgg gcaaatttct gcgctactac cgcatcacca agcatgaccg gaataatggc
     9961 gtgatcggct cccgctaggg taaagcccgc cgccgacatt tgctcacgga actgacgcgc
    10021 gttcgcccag agacggtcac gcagctcgct acccgcttca accatctcca gcactttgat
    10081 ggaagcggca acaatggccg gtgccagcga gttggagaac agatacggac gagaacgctg
    10141 gcgcagccac tcaaccactt ctttgcgcgc cgcggtataa ccaccagaag ccccgcccag
    10201 cgctttaccg agcgtgccgg tgataatatc gacccggccc atcacatcgc agtattcatg
    10261 ggaaccacga ccgttttcac cgacaaaacc taccgcgtgg gagtcatcta ccatcaccag
    10321 ggcatcatat ttatctgcca gatcgcaaac gcctttcaga ttggcaatca cgccgtccat
    10381 cgagaacaca ccatcggtgg caatcagcac atgacgcgca ccggcttcac gcgcttcttt
    10441 cagacgtgct tccagctcct gcatatcgtt gttggcataa cgatagcgtt tagctttgca
    10501 cagacgcaca ccatcaataa tagacgcgtg gttcagtgcg tcggagataa tggcgtcttc
    10561 cgcgcccaac agcgtttcaa acaggccacc gttagcatca aagcaggaag agtagagaat
    10621 cgcatcttcc atccccagga aggccgccag tttttgttca agctctttat ggctgtcctg
    10681 agtgccgcaa ataaaacgca ccgaagccat gccgaaaccg tgagaatcca ttcccgcctt
    10741 tgccgccgca atcagatccg gatgattcgc cagtccgaga tagttattgg cacaaaagtt
    10801 aatgacgtgg cttccatcag ccacagtgat atctgcttgc tgcgcagacg taataatgcg
    10861 ctcttcttta aacaaccctt ccgcccgtgc ggtttccaga tcgttggtta actgctgata
    10921 aaattctcca cgcattgcga ttctccagac agggcaaatt ccagcacata ttacccaaac
    10981 ttataggttg cgacgagatg acgcgttaac acttctgcaa aattcaggat aaataacgtg
    11041 aatcttgatg tctctcaaca cctatagata gtagaaaaac tgtacagttt tggctgggca
    11101 taatgatgag tgattttact gcattgatat aaaaagaaaa aatgatgcca aagcatcatt
    11161 ttttcctatc tcagttggca tcaaaacgct acccactaat ggagggaaac ctattttctt
    11221 gaaaggaaaa ttctcagcgc ctgcattttt gcccctaaag tgttactcct gaagcagcga
    11281 aacaaatcga accattttcc tctccccaga tagtttaggt tgaagagatc ggaacgtttg
    11341 taatagcaca tgacaaagat gccctggtca tcgtccacaa tgttattgtt tagggttatt
    11401 ttttgacttt ccagaactaa tttatagaat tctttccatt tatgctgtga accaacaatt
    11461 gcgccaccaa taatatatac atgattgcca atcatgaaat caaaagcctg ttgttgagat
    11521 gtgacagtaa gccctttttt tatagtgaaa agatgcatct tattttcatc aaatgggaaa
    11581 tcccaaattt tcaatcctcg agttacattt ggcttacggc aatagccaaa atctatccac
    11641 gcaacaagag gcgttttcac taagcccatg ttgatagctt tatttacaaa ataggcctta
    11701 aggttacata ccaaaacata ctctggtgac cagtactctg ggttttttaa ttgtcgaggc
    11761 tcaagtctat ttgtaaacga ctcatctttt tgaatttttt cgatacgact tctgatatat
    11821 ctaaatttct tttttatatc gataacgata accgttgttg gttttccgtt gcgaatcgct
    11881 tcaacccttg atttcaaatc gggagaagtg aaaataatca tctcattttc aagtgcggcc
    11941 agacgctcaa agtaactaaa ataaacatca actgaacgag ctagtttttc acggaaccct
    12001 ttattagcag tccaatcccc tctaccaatg tcaaaatatg cagtgataat tgtggtggat
    12061 gatttcatta ctaataagac cattaatata aaaaaagaat tatatcataa atattaacag
    12121 aatgtatact gtatattatc cattcaaatg tttcagttat ttatcataca tcggaatatt
    12181 gatactaaag cactattcac gtgcaaatcc aacatccgcc atgaaggact ggctaaaacc
    12241 caaactagtt tgttgcaatt agcatccttg catctctatg taaagggctg aagggattcg
    12301 gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc cataattcga
    12361 aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc aacatcgtta
    12421 aagccctgaa tgacaaaggc atcaccgata ttctggtggt ggacaacctg aaagacggca
    12481 ccaagtttgt gaacctggtg gatctggata tcgcggacta tatggataag gaagacttcc
    12541 tgatccagat tatggctggc gaagagttcg gcgatgtcga agcgattttc cacgaaggcg
    12601 cgtgctcttc caccaccgag tgggacggca aatatatgat ggataacaac tatcaatact
    12661 ccaaggagct gctgcactac tgccttgagc gcgaaatccc gttcctgtac gcttcttctg
    12721 cagccactta cggcggtcgc acctccgact ttattgaatc ccgcgaatat gaaaaaccgt
    12781 tgaatgtcta cggttactca aaattcctgt ttgatgaata tgttcgccaa atcctgcctg
    12841 aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc
    12901 ataaaggtag catggcgagc gtcgctttcc atctcaacac tcagcttaac aacggtgaat
    12961 caccgaagct gtttgaaggt agcgagaact tcaaacgtga cttcgtctat gtgggcgacg
    13021 tagcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggtatcttc aacctcggca
    13081 ccggtcgtgc agaatccttc caggcagtag cagatgctac gcttgcttat cacaagaaag
    13141 ggcagatcga atacattccg ttcccggata aactgaaagg ccgctatcag gcgttcactc
    13201 aggctgatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa accgttgctg
    13261 aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc tgcatgaaaa
    13321 tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt ctctatcgca
    13381 cgctccaggc gcgctatccc caggcgataa tcgacgtgat ggcaccggca tggtgccgtc
    13441 cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc ggtcacggag
    13501 cgctggaaat cggcgaacgc cgcaaactgg gtcatagtct gcgtgaaaag cgctacgacc
    13561 gcgcctacgt cttacccaac tccttcaagt ccgcattagt gcctttcttc gcgggtattc
    13621 ctcatcgcac cggctggcgc ggcgagatgc gctacggttt acttaacgat gtacgcgtgc
    13681 tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc tatgacaaag
    13741 gcattatgcg tacagcacaa gatctgccgc agccattgtt atggccgcag ttgcaggtga
    13801 gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa cgtccgatga
    13861 ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac taccactatg
    13921 cggaactggc aaagcagctg attgatgaag gttatcaggt ggttctgttt ggctcggcga
    13981 aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag caggcatggt
    14041 gccggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt gcagcctgta
    14101 aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc
    14161 tggttgccct gtatggtccg agtagcccgg acttcacacc gccgctatcc cataaagcac
    14221 gcgtgatccg cctgattacc ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt
    14281 atcaccagag cttgatcgac attactcccc agcgcgcact ggaagaactc aacgcgctat
    14341 tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg
    14401 atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca gggattaagt
    14461 ttgactgggt ggtggaagaa gggttcgcac aaattccttc ctggcacgcc gctgttgagc
    14521 gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggct cccataaaag
    14581 ctgaacggaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca gttatcgacg
    14641 ctcaggggct ggtaaaaagc gcggcgctgg tgacgcgtct ggcacatggc gtaaagcatg
    14701 gaatggactg gcaaaccgct cgcgaaccac tggcaagctt gttttacaat cgtaagcatc
    14761 atattgcgaa acagcagcac gccgtagaac gcacccgcga actgtttgcc aaaagtctgg
    14821 gctatagcaa accgcaaacc cagggcgatt atgctattgc acagcatttc ctgacgaacc
    14881 tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt gatgataaac
    14941 actggccgga agaacactgg cgagatttga ttggtttact ggcagattca ggaatacgga
    15001 ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg gcggaaggat
    15061 ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc gtgctggccg
    15121 gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg gcactggata
    15181 gacccaatat cacggtttat ggacctaccg atccgggatt aattggcggg tatgggaaga
    15241 atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct caaaccgttt
    15301 ttcaaaattt aaatcttgaa ataataacca ataagttgac atcggagata agatgacctc
    15361 aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca gagctctcgt
    15421 attcttattc attaccacct attttttggg tgggattaca aggtataaac atcttattgt
    15481 tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac actatctttc
    15541 actatttaaa acatgtcttt ttggtagtgt tgccatatta actattgctg cattgctatc
    15601 acttcttcaa tccccagatg caggtgctag catgaaggaa attttcaaag ctatcattga
    15661 aaatacttta ctatgcacaa tagcaatacc ggtcatattg agagacgaga aaagagaaga
    15721 tgtcgaaaaa atcgttttct tctcatttat aagtgcgttg ggcttacgtt gtttttctga
    15781 attgattgcc tattataagg actatcaaca aggggtaatg ccatttgcag attatagaca
    15841 ccgtagcatt tctgactcga tggtcttttt atttcctgca ttgttaaatc tctggcttat
    15901 caaatcagca aaataccgca tttcttttgc ggttctaagc gttattttta tttttctgat
    15961 attaggaact ttatccagag gagcctggct ttccgtgtta gtcattggat taatatggat
    16021 tctgatgttt aaacaatgga agttactatt agtaggagta atggtttcca tcattgcatt
    16081 atcggttatt ttcacacata aggagatgac cgcaaatcta acgtataaac ttcaacaaac
    16141 taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc taatattaga
    16201 aaatcctgtt attggttacg gttacggtaa cgttgcatat aaagatgtct ataataaacg
    16261 tgtcgttgat tatccagaat ggacctttag acaatcgata gggccacata attttgcgct
    16321 attcatctgg tttggcactg gtttattagg gctgataagt cttatgatgc tatactgcgc
    16381 aatattgaaa gagtgtataa aaaatggcgt ccagaataaa aaccgctcac catataatgc
    16441 atattatata atcttactat cttttatagg ttattttgtt atccgtggaa acgtagaaca
    16501 aattgaacca aatttattag gcgtttacgc cggcttatta ttagcaatga aaaacaagta
    16561 aaaaaaataa aaaaggctgc ataatgcggc ctttttattt attcaaacca attatgaatg
    16621 acctcttcga atctttgagt tacgccatcc caactgtagt gatcaaacac aaaatcctga
    16681 ccttgctttg caactgctgt taattcagga ttcgctaacg tttttaaaat atcgctgctt
    16741 atggagtctg cagtcatagg ttcttttaaa tggaatcccg tagtattttc tttgacaaat
    16801 tccgtcatcc ccccacgcgt actcactaaa acaggcttac cagcccccat agcttcaatt
    16861 gccaccatgc aaaatggttc ctggaactgg gaaggaatga caactaaatc tgccagtggg
    16921 taataacaat gcatcttttc aggcggtatg cttcctaaca taatgcaacg atccttcaaa
    16981 cgttttgcaa tttccctgac attgtgttga tatgcacctt tgtcactttt actcatttct
    17041 gtataatcac caatcacaac taattttaaa tttttgtgag caacggccaa tttttcgaat
    17101 gattgcatca acagtaatat gcctttgtca gggacgatac gcccagcata aaaaataatt
    17161 ttctcttcag gggagatatt aagttcgctt ctagtgatcg gctgaaaagt agattgatag
    17221 gtttctaaat caattccatt tggtacaatc tcaatatctg catttgctaa ataagattgg
    17281 taatactttt tcaagtataa actaggcact atcattttaa cattttgctc aagcaatttt
    17341 ggttcaaatg cattatgcat atgaataacc actcttgcct gaggagctcg cttacgaatt
    17401 tgagtgtata gcttcatgct attatgaact acaataatac tttctttcgt tataggaaaa
    17461 tcatgggcaa tattcaatat tctctgtgaa tatggtagag gatcaagtct tgtccatttt
    17521 tgaaataacc gcttatatat gcgactgaat ccaaccctat gaatactaca attatcatta
    17581 acaaaggtgt aatttgagta tcctggattc ttgatacaaa caatgcgatt tgggaaattg
    17641 gtacgctgag caacctgata catccacgtt tcaacagcgg ccgctccgcg gggaggaatt
    17701 gaaa
//
LOCUS       DAYPHN010000098        17699 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000098 DAYPHN010000000
VERSION     DAYPHN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.098"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(92..2539)
                     /gene="glgP"
                     /locus_tag="ACG0BW_003092"
     CDS             complement(92..2539)
                     /gene="glgP"
                     /locus_tag="ACG0BW_003092"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFK7260022.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPELMRL
                     LIDEHQFSWDDAFGVCCEVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLIDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2558..3991)
                     /gene="glgA"
                     /locus_tag="ACG0BW_003093"
     CDS             complement(2558..3991)
                     /gene="glgA"
                     /locus_tag="ACG0BW_003093"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFK7260023.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3991..5286)
                     /gene="glgC"
                     /locus_tag="ACG0BW_003094"
     CDS             complement(3991..5286)
                     /gene="glgC"
                     /locus_tag="ACG0BW_003094"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFK7260024.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5304..7277)
                     /gene="glgX"
                     /locus_tag="ACG0BW_003095"
     CDS             complement(5304..7277)
                     /gene="glgX"
                     /locus_tag="ACG0BW_003095"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFK7260025.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCIFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7274..9460)
                     /gene="glgB"
                     /locus_tag="ACG0BW_003096"
     CDS             complement(7274..9460)
                     /gene="glgB"
                     /locus_tag="ACG0BW_003096"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFK7260026.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKVGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDTSLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9733..10836)
                     /gene="asd"
                     /locus_tag="ACG0BW_003097"
     CDS             complement(9733..10836)
                     /gene="asd"
                     /locus_tag="ACG0BW_003097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFK7260027.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTDTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11028..11621
                     /gene="yhgN"
                     /locus_tag="ACG0BW_003098"
     CDS             11028..11621
                     /gene="yhgN"
                     /locus_tag="ACG0BW_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFK7260028.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11678..13018)
                     /gene="gntU"
                     /locus_tag="ACG0BW_003099"
     CDS             complement(11678..13018)
                     /gene="gntU"
                     /locus_tag="ACG0BW_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFK7260029.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13022..13549)
                     /gene="gntK"
                     /locus_tag="ACG0BW_003100"
     CDS             complement(13022..13549)
                     /gene="gntK"
                     /locus_tag="ACG0BW_003100"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFK7260030.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13688..14683)
                     /gene="gntR"
                     /locus_tag="ACG0BW_003101"
     CDS             complement(13688..14683)
                     /gene="gntR"
                     /locus_tag="ACG0BW_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFK7260031.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14907..15602)
                     /gene="yhhW"
                     /locus_tag="ACG0BW_003102"
     CDS             complement(14907..15602)
                     /gene="yhhW"
                     /locus_tag="ACG0BW_003102"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFK7260032.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVYQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15725..16762)
                     /locus_tag="ACG0BW_003103"
     CDS             complement(15725..16762)
                     /locus_tag="ACG0BW_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFK7260033.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17097..17585
                     /gene="yhhY"
                     /locus_tag="ACG0BW_003104"
     CDS             17097..17585
                     /gene="yhhY"
                     /locus_tag="ACG0BW_003104"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFK7260034.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4363 a         4815 c         4409 g         4112 t
ORIGIN      
        1 gtcatgacat atcgtttatc tgatataaaa atgccgtgtg ccccttaacg atagctttga
       61 gggaacacac ggcagttatc actttgcaaa cttacaatct caccggatcg atatgccaga
      121 tatgatcagc gtactctttg atcgtacggt cagaagagaa gtagcccata ttggcaatgt
      181 ttagcatcgc ttttgcggtc cactcttcct gacgctcgta gagttcgtcg actttatcct
      241 gacaatcgac ataactgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      301 gcgaatcaat cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      361 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      421 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      481 caccgacatg atccagcatc tcgacattcg caccgtccag cgtaccgatg gtcagcgcgc
      541 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccccgcc agcgaaatct
      601 gttcagacag atcggccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      661 tgaacacgac tttcagcttg tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      721 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      781 aaatattcac gcgcggtacc catttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      841 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttaatttgca
      901 catcgaacag cgctttcgga tttaccacca cattcagctg ctgggcgata tactccgcca
      961 gacgcttttt gttctccagc ttcgcctgat gcaccgcgtg attaaccatt gggaaatcac
     1021 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1081 cgtccagcac agctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggggtcacac
     1141 cgttggtaac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1201 gcaccatcag gttagagtgc agttccgata caccgttaac tttgtggctc accacaaccg
     1261 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1321 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttaattt
     1381 caaagatgat ctgtagatga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1441 ccagcgcctc gctcatcagc gtgtggttgg tgtaagagaa aacctcgcaa cagaccccaa
     1501 acgcgtcgtc ccaactaaat tggtgctcat caatcagcag gcgcatcaac tcaggaatcg
     1561 acaacaccgg atgggtatcg ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1621 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaaat
     1681 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcgtccgga tacagtacgc
     1741 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1801 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1861 cgtcagtgtc gtaacccggg ataatctgat cgtaagccac tcccagaatc tcttcggttt
     1921 caatccagcg cgttttttta ccttcctgct gaatgcgacc gccaaaacgg actttatagc
     1981 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tcaggtgact
     2041 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2101 aaccgcgccc cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc gccagacgtc
     2161 ccaggccacc gttaccgagg cccgggtcat tttcttcatc aatcagctct tcgagattta
     2221 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2281 tggaaagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2341 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2401 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2461 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2521 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2581 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2641 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca
     2701 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2761 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2821 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2881 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2941 cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     3001 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aaggcccggt aaggcttcca
     3061 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3121 tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt
     3181 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3241 tctcgtccac gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca
     3301 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3361 ctgccgtaat gtgatcggca tagtacagac cggccttcag aaaagagatt tgtccgttga
     3421 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3481 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3541 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3601 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3661 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3721 gacgatcata gagatgcggc gcgtcaatca ggtaaatgcc aaccccgttg taatgaccga
     3781 acaacagcgt gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtca
     3841 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3901 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3961 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     4021 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4081 ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac
     4141 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4201 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4261 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4321 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4381 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta
     4441 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4501 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4561 tttgccaaag tcgtggctgg agttctcatc gcgatcgtct tcttccagca gttcatacag
     4621 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4681 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4741 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4801 acgtgcgcct ttttcgacgt ggtcgataag catacgcgag tagtcttgct tgtagatatg
     4861 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4921 ggtaactgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4981 cagcagatcg acaaactcat tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     5041 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga taccggagtt
     5101 gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg
     5161 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5221 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5281 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5341 tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5401 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5461 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5521 ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc
     5581 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5641 ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg
     5701 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg
     5761 gagagcaaca acgttgttaa cagggcgtga atgctgtcgc gacgccgttc aaccaggtca
     5821 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5881 cgattttctt ctccgtttgc ttcattatgt ttatggttga agcaaacgca gtcgcgaagc
     5941 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     6001 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6061 tgaagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6121 gggaaatttc ccacctgata accaccagga gcaatatccc acggttcagc aattaacttc
     6181 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6241 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6301 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6361 agcgtgttgc cacaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6421 ctacggttgt cgatcccacg cagagagaat aacggaccgt cgaggtccag ttccgcacta
     6481 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6541 tcgcgaaact catccagcgc cgtttctggg gagcaggcat acgccggatg cagtgcaaac
     6601 atcgccaccg ggttgtaacc ccagtagtta cttagaccca tacgttgcag gcgtggttcg
     6661 ctggcaaact gcgccaccgg cagcagttcc agcgcggtaa tgcccaactg tttcaaatag
     6721 ttgatcatca ccggatgccc cagggcttta taagtgccac ggatctcgac cgggatctcc
     6781 gggtgcaggt acgttaatcc tttgacatgg gcttcataaa tgatggtact gccccacggc
     6841 gtgcgcggcg gggcatcatc ctcccagtca tagtgatcaa ccaccactac gcatttcggc
     6901 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6961 tctttaaatt ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     7021 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7081 ggacgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag
     7141 cgatgttcct ggccattggc gtcaaagata cacagttcta cccgctcggc atgagcggag
     7201 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggtgc gggtttgcca
     7261 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7321 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7381 cattgccggc attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt
     7441 tgcccggctg gttaatgccg aagcgataat catgacgcgg caccggagta aagttactgg
     7501 caacgatgat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7561 tgtcatccac caccagccat tcaaaaccgt acgggtcaaa atccagttca tgcatcgctt
     7621 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7681 tatcgccgcc ttccaacaga tgccagtcga ggctggtgtc atggttccac tcgcggccct
     7741 gggcaaattc gttccccatg aacagcagtt tcttgcccgg gaatgcccac atccagccgt
     7801 agtaggcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatccgg tcgagaatcg
     7861 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7921 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaacc gggtcgagct
     7981 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     8041 cgcccatgtc ctgcggacga gaaacgccag ggaaatcggt agactcctcc gccattgtca
     8101 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8161 caagattctc gcgcccgcca aattcgttcg ggatccactc ccccacttta cggctgtagt
     8221 cacgataaat cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcga
     8281 tccagtaaag cgcgttaccg acaaggaagt tactgacttc acggcgacca tagttgtaga
     8341 tcagcgtatt ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg
     8401 tgccatcaaa ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggc acccagtcga
     8461 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcggg
     8521 taccaaaacg gcgggtcggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8581 acggatgctc gttaatgggc agcagttcga ggtgggtaaa gcccatccat ttagcataag
     8641 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8701 gccaggagcc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8761 tgcgctcttc agtctgcaca accttttccg gtagtccgca aataagagac gcggtttccg
     8821 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8881 caatcatctc atatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8941 tgccgctctc tttacgcagg cgcatcggat gacggcgacc gtcccagtag ttgaattgcc
     9001 caaccaccga gacccgacgg gcgtttggag cccagacaga aaaacgcgta cctgtgacgc
     9061 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9121 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatctatca
     9181 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9241 gaatgacacc gctaaagaat ccacgggagt cgagacactc cagttttgcg agtttgcgcc
     9301 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9361 gtcccgcggt ggttttatgc atccccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9421 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9481 gtgtcacccc atccaactgg atttttgact tctggttgtg acattttttt gacctgaacg
     9541 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9601 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9661 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9721 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9781 cagcagctgg tcacccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9841 cagacggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9901 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgtgcag ccagcagttc
     9961 ttccacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
    10021 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10081 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10141 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10201 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10261 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtca ataactcacg
    10321 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10381 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10441 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10501 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10561 accttgccat ccgctttcac gaagcttcgg atagatttcg ttggtataat cgccgccctg
    10621 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10681 gtcagtagtt ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag aaaagaagac
    10741 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10801 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10861 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10921 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10981 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    11041 tttctgcagc agttttattg atcctgatta tggatccgct cggaaaccta cctattttca
    11101 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11161 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11221 ttagcctgcg ggcagaaacc gtctccattt ctggcggcat cattctgttt ctgatcgcca
    11281 ttaagatgat tttccccagt gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11341 cgtttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11401 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtaattgct ctgctactgg
    11461 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11521 agaaaggggt gaatgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc
    11581 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11641 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaaagcaa
    11701 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11761 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa
    11821 cgtggctgac aacaatcgaa ccaccggcga tacaaatcga cagcgccgcc atttgcgcac
    11881 cggagtagtt cagttgttca ataaccggca tcaccagtcc caccgccgtt aaacaggcaa
    11941 cggtggcaga accctgaatg atacgcaccg ccgcagccag cacaaagcag gtgatggcaa
    12001 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12061 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12121 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12181 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12241 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccta
    12301 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttgcctt
    12361 cgccgagatg cggttcgctg atgtcgtcag gaatatgcaa ctccacgtag cggctgatga
    12421 aattacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12481 ggatcatcca gccaaagtcg gcgttcatct gcgatgccag cagcattggc gctggccccg
    12541 gtaccaggaa cgcagccgcg gccgccacgc ctgcgaataa tgggattacc agtttcacca
    12601 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12661 cttcaaagaa cagcggtagc gcacaaacca gccccgcaag gccgatggca taatgtgctc
    12721 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccagtttcat
    12781 gtaagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12841 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12901 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12961 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    13021 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13081 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13141 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa
    13201 tcacatcaaa atcgcctttc aggtagatga acgagagatt cggattacct tcacgcagca
    13261 agtcgcgata gtgttttttc agtgcagaac agacgatcag cgacacttta ttggtgcgct
    13321 gcatagcaaa cgcggcgtcg ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca
    13381 gcggttcacc agacgccatt ttttcgatat tgcgccttgg atggaggaaa tcgccatcaa
    13441 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13501 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13561 cacaatacaa gaaataaaaa tgttacgggt aactgttatc ggtaacattg tccggccgga
    13621 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13681 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13741 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13801 cacgcggcgt cagtacgctc gccaaccgtg gttccatcac ctgaccaatg tcatgaccat
    13861 ggaaaccggc aatcgccata tcgtcaggaa ctttcaaccc cagacgctgg cattcaaacg
    13921 ccgcgccgac cgccaggtca tcattagtac agaacacgcc atccagctgc ggatattccc
    13981 gcctcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    14041 cgctatacgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14101 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa
    14161 tggcggtggt catctggcgt gccgcttcaa agttatcaaa accgacggca atatcgaggc
    14221 acggcgactt gctgtccatc aactccacca ccggaatccc cgccacttca atcatcttta
    14281 aggtgcgtgg cgtgtgggta cgttcggtga ggatcagtcc atcgatattc catgagagca
    14341 ttgattcgag gcgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatagttt
    14401 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14461 tggtgagaga aggtaacagg acgccaatcg cccggctggt ggcattagag aggatatcgg
    14521 gcgcgcgatt gggaatataa cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14581 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14641 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14701 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14761 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14821 caaaaccccg gaagcgtaca tcagcacgcg accggagcga gtgaggaagc ctgcaaaaag
    14881 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14941 ctgtcgctat ccgcatgaat ggagattgcc tgctcatccc agattgccag accatcgctg
    15001 gtcgaggctt tcacgccgtt aatggtgaca ttgcctttca ccacctgaat ccagacgcgg
    15061 cgttcagcgg caatctgatg caccgactgc tcatctttta ccagcgccca gcggtacagt
    15121 tccatatcct gatacacttt cagcgaacca tcacgcgcat ccggcgagag caccagctgt
    15181 ttgccctgta cggcatcgaa gcgacgctgt tcataacgcg gcgtaatacc gttttcttca
    15241 ggcatgatcc agatttgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15301 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15361 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15421 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15481 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc
    15541 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15601 atttttattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ccgcctggga
    15661 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15721 aaacttactt cgcgagagtc actgtggagg gagaggcttg ctcaaatcct cgttcaagga
    15781 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg ttggtgatgg
    15841 tttgatacaa cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc
    15901 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15961 atcccggttc gcccggcata atattggcct tgaggctggt ttcctgctgg tcgataccgt
    16021 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16081 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagctgcgct tcaaaggtgt
    16141 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16201 cgaacagaga aataatctgg tccatcgtat gcacaccaag accatagaac gcgccatcct
    16261 gcggcagccc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16321 cgacaatctc tccaagcttg ccgctttcaa tcgctttttt cgctgtcagg aagcaggaat
    16381 caaagcgacg attctgatac ggcgtgacgg tcagcccttt gcttttcgcc aacgcaaaca
    16441 gctccttcgc ctgggcaagc gtcggggtga acggtttttc gaccagcaca tttttcccgg
    16501 cttccagcgc gcgcttcgcg tactcgaagt ggctgtctgc atgggtgcag accacaacca
    16561 gcttaacatc gggatcgttt agtacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16621 aaatgggggc ctgttcttcc ggcttcgcat ggcggcgaaa aatatgcgcg acatgccagc
    16681 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16741 taaaggcgca gttaatgacc atgatttcgt cctttttaag ggggttattt acaccttagc
    16801 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact tcccgtggat
    16861 aaattgaaaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16921 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca
    16981 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaaat gcatttgact
    17041 cgcatttgaa agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga
    17101 gtgagatagt gatacgccat gctgaaacgc gggactacga ggccatcagg cagattcacg
    17161 cccagccgga ggtgtattgc aacacgctac aggtgcctca tccctccgat catatgtggc
    17221 aggagcgact cgccgatcgc cccggcatca agcagttagt cgcctgtatt gatggaatcg
    17281 tcgtaggcca tctcaccatt gacgtgcaac aacgcccacg ccgcagtcat gttgccgatt
    17341 ttggtatctg tgtcgactcc cgttggaaga atcgcggtgt cgccagcgcc ctgatgcgag
    17401 agatgattga aatgtgcgac aactggttgc gggtagatcg tattgagcta acggtgttcg
    17461 tcgataacgc gccagcaatt aaggtctata aaaaatttgg atttgagatt gaagggactg
    17521 gcaagaagta cgcattacgt aatggtgaat atgtcgatgc gtattatatg gcgcgggtga
    17581 agtaagatag tgcccttttt ctgagatgga aaaagggtgt cattcaaaat cggcatacct
    17641 tcctttaagg tatttatttt cccaataaat atataagatt tacctcataa ttacgtcct
//
LOCUS       DAYPHN010000099        17646 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000099 DAYPHN010000000
VERSION     DAYPHN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.099"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            118..372
                     /gene="bssS"
                     /locus_tag="ACG0BW_003105"
     CDS             118..372
                     /gene="bssS"
                     /locus_tag="ACG0BW_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFK7260035.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYQRH"
     gene            487..1605
                     /gene="solA"
                     /locus_tag="ACG0BW_003106"
     CDS             487..1605
                     /gene="solA"
                     /locus_tag="ACG0BW_003106"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFK7260036.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGDYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1653..1766
                     /locus_tag="ACG0BW_003107"
     CDS             1653..1766
                     /locus_tag="ACG0BW_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFK7260037.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2027..2593
                     /gene="yceJ"
                     /locus_tag="ACG0BW_003108"
     CDS             2027..2593
                     /gene="yceJ"
                     /locus_tag="ACG0BW_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFK7260038.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2597..3172
                     /gene="yceI"
                     /locus_tag="ACG0BW_003109"
     CDS             2597..3172
                     /gene="yceI"
                     /locus_tag="ACG0BW_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFK7260039.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDDLDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3214..4266)
                     /gene="trhO"
                     /locus_tag="ACG0BW_003110"
     CDS             complement(3214..4266)
                     /gene="trhO"
                     /locus_tag="ACG0BW_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFK7260040.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4491..5411
                     /gene="lpxL"
                     /locus_tag="ACG0BW_003111"
     CDS             4491..5411
                     /gene="lpxL"
                     /locus_tag="ACG0BW_003111"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFK7260041.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5583..6809
                     /gene="mdtG"
                     /locus_tag="ACG0BW_003112"
     CDS             5583..6809
                     /gene="mdtG"
                     /locus_tag="ACG0BW_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFK7260042.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKKP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6892..7266
                     /gene="msyB"
                     /locus_tag="ACG0BW_003113"
     CDS             6892..7266
                     /gene="msyB"
                     /locus_tag="ACG0BW_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFK7260043.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPSIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7267..7494)
                     /gene="yceK"
                     /locus_tag="ACG0BW_003114"
     CDS             complement(7267..7494)
                     /gene="yceK"
                     /locus_tag="ACG0BW_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFK7260044.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQSYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7667..10210)
                     /gene="mdoH"
                     /locus_tag="ACG0BW_003115"
     CDS             complement(7667..10210)
                     /gene="mdoH"
                     /locus_tag="ACG0BW_003115"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFK7260045.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDADHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10203..11738)
                     /gene="mdoG"
                     /locus_tag="ACG0BW_003116"
     CDS             complement(10203..11738)
                     /gene="mdoG"
                     /locus_tag="ACG0BW_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFK7260046.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12132..13289
                     /gene="mdoC"
                     /locus_tag="ACG0BW_003117"
     CDS             12132..13289
                     /gene="mdoC"
                     /locus_tag="ACG0BW_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFK7260047.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKTLFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13297..14778)
                     /gene="clsC"
                     /locus_tag="ACG0BW_003118"
     CDS             complement(13297..14778)
                     /gene="clsC"
                     /locus_tag="ACG0BW_003118"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFK7260048.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14720..15253)
                     /gene="ymdB"
                     /locus_tag="ACG0BW_003119"
     CDS             complement(14720..15253)
                     /gene="ymdB"
                     /locus_tag="ACG0BW_003119"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFK7260049.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLSAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENTHLYERLLTQQGDE"
     gene            complement(15348..15659)
                     /gene="ymdA"
                     /locus_tag="ACG0BW_003120"
     CDS             complement(15348..15659)
                     /gene="ymdA"
                     /locus_tag="ACG0BW_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFK7260050.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15780..16112)
                     /gene="csgC"
                     /locus_tag="ACG0BW_003121"
     CDS             complement(15780..16112)
                     /gene="csgC"
                     /locus_tag="ACG0BW_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFK7260051.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16171..16626)
                     /gene="csgA"
                     /locus_tag="ACG0BW_003122"
     CDS             complement(16171..16626)
                     /gene="csgA"
                     /locus_tag="ACG0BW_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFK7260052.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16667..17122)
                     /gene="csgB"
                     /locus_tag="ACG0BW_003123"
     CDS             complement(16667..17122)
                     /gene="csgB"
                     /locus_tag="ACG0BW_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFK7260053.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4447 a         4461 c         4158 g         4580 t
ORIGIN      
        1 tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag ttgcttcaga
       61 taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg ggccatgatg
      121 gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat cagcaccgtt
      181 gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa gtccgagcag
      241 gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag acaatttatt
      301 tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt aaacgagtat
      361 cagcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt gcctttacca
      421 tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga gagcagagag
      481 aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc tgccgccggg
      541 tattatgcaa cccgtgccgg tttaaacgtg ctaatgaccg acgcccatat gccaccgcat
      601 caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa
      661 aagtatgtcc cgctggttct ccgcgcgcaa acgctgtggg atgaactctc ccgccacaac
      721 gaagaagatc ccatttttgt acgctctggc gtcattaacc ttggcccggc tgattccaca
      781 tttctcgcca acgtcgccca cagtgccgaa caatggcaac tcaacgttga aaagctcgat
      841 gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta tatcggctta
      901 tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg gatccaactg
      961 gcgaaggaag cgggctgtgc gcagctgttc aactgcccgg tcaccgcaat tcgtcatgac
     1021 gatgatggcg taactattga aacggctgac ggtgattatc aggcgaaaaa agcgattgtc
     1081 tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc cgtacgcaaa
     1141 gtatttgcct ggtatcaggc cgatggtcgc tatagcgtga agaataaatt cccggcgttt
     1201 accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa cgacgcgttg
     1261 aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg tgttccgttt
     1321 gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt
     1381 atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga cgaagatttt
     1441 attatcgata ctctacccgg ccacgataat acactgctca ttaccggcct gagcgggcac
     1501 ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca agacaaaaaa
     1561 agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc attacggcct
     1621 ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac tatctcatca
     1681 ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg gcaattatgg
     1741 atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac aaatatttaa
     1801 tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
     1861 cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta ttaattcaaa
     1921 attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc
     1981 cgacacgcca ttttctttct ctcgcaaatt cgttttggat gcacttatgt cattcacaaa
     2041 tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt
     2101 ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta
     2161 tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat
     2221 tcgcgttctg tggcgcgtca tatctccacc gccgggtccg ctgcagagtt attcgcccat
     2281 gactcgtctt ggcgcaaagg ctggacatct cgcattgtac ttattgctgt tcgccatcgg
     2341 catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt
     2401 tgacgtcccc gcgaccctcg ccgacgctgg cgcacaggct gactttgccg gtgccctgca
     2461 tttttggctc gcgtggagcg tcgtagtact gtccgttatg cacggattta tggccctgaa
     2521 gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga
     2581 ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgctt ccctgatgtt
     2641 ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt
     2701 tgttaacttc cgcatccagc atcttggcta tagctggtta tatggcacct ttaaagattt
     2761 cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga atgtgacaat
     2821 taacaccacc agcgtcgata ctaatcacgc cgaacgcgat aaacatcttc gcagtgcaga
     2881 tttcctcaat accgcaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga
     2941 cggtgacgac ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac
     3001 gctggaagcg aaattaattg gtcagggcga cgacccgtgg ggcggtaaac gtgcaggctt
     3061 tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt taggtccagc
     3121 ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa
     3181 tgttgccgga tggcacgcat ccggcaatga tatttattcc gttggatcag gaatgcccag
     3241 tgttgtattc aaacgcccac gagacttatt aaagatctta ttgccatttt cacgtcctgc
     3301 ccgacggcgt cgctgttctt caggcggtaa cgcgctttct tcgcagcaaa tctcactaca
     3361 acaacctttg tatttttccg cacatactgg acactgaata aaaagcaggt ggcagccatc
     3421 atttttacag ttggtatggc tgtcgcacgg cgcaccgcac tggtggcaat gcgcgataat
     3481 ctcatcagat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg
     3541 taccggtaaa ccctgctcgc gcgccttgcg ggcgtattca ataattccgc cctcgatatg
     3601 ccagacttta ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc
     3661 gccggtgcag tacataacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt
     3721 tggcagctgc tcacggaagg tatctgccgg aatttccagc gcgttttcaa agtgccccac
     3781 ttcatactca tagtggttac gcatgtcgat aaacaatgca tcgggatcgt caagcatagc
     3841 gttcacttcc gccgcttgca gatactcacc cacattgctg gcatcaaagt gaggatcgtc
     3901 aataccgtcg gcaacgatac gatcgcggac cttcatgcgc agtacccaga aggatttccc
     3961 gtcatcatcc aacgcgatat tcaggcgtaa gccctctaaa gccgggtcga aggcataaag
     4021 ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc
     4081 atgcgccaga tacactcgcc caaaaacatc cagcgcggta aacagctgat ataaagcgtc
     4141 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
     4201 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
     4261 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
     4321 ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt
     4381 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
     4441 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
     4501 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
     4561 gcgtactttg gttagtcgtg caattgccct acccggttat ctaccgcctc ggttgtggat
     4621 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
     4681 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
     4741 aatccgttgg catgggactg atggaaactg gcatggcgtg gttctggccg gaccgcagaa
     4801 tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
     4861 gcggcatcct gttagttggc atccattttc tgacactgga gctgggtgcg cggcagtttg
     4921 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
     4981 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
     5041 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccggat catgattacg
     5101 gcccgcgctc aagcgttttc gtcccgttgt ttgccgttga gcaggctgcg accacgaccg
     5161 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
     5221 agccagatgg caaagggtat cagttgatta tgctgccgcc agagtgttct ccgccgctgg
     5281 atgatgccga aaccaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
     5341 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
     5401 cacgctatta aatctcccat gccggatgct tcagaagggt atccggcatt accacagcaa
     5461 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
     5521 attagtgtgc ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
     5581 ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct
     5641 ggctaggctg ttttcttacg ggtgccgcct tcagcctggt aatgcccttc ttacccctct
     5701 acgttgagca gcttggcgtt acgggccact ccgccttgaa tatgtggtcc ggtattgtct
     5761 tcagcattac atttttattt tcggccatcg cctcaccgtt ttggggtgga ctcgccgacc
     5821 gtaaaggccg aaaactcatg ctattacgct ctgccctcgg catgggcatc gtgatggtgt
     5881 tgatggggct ggcacaaaat atctggcagt ttttgatcct acgggcgctt cttgggttac
     5941 ttggcggatt tgtccccaac gctaatgctc ttatcgccac acaagtacca cgtaataaaa
     6001 gcggctgggc gctggggacg ctttccacag gcggcgttag cggtgcgttg ctcggcccaa
     6061 tggctggcgg tttgcttgcc gatagctacg gcttacgtcc ggtattcttt attaccgcca
     6121 gtgtgctcat actctgcttt ttcgtcaccc tgttttgcat cagagaaaaa ttccagccgg
     6181 tcagcaaaaa agagatgctg cacatgcggg aagtggtgac atcacttaaa aaaccgaaac
     6241 tggtactcag cctgtttgtc actacgttaa tcatccaggt ggcgacgggc tcaattgccc
     6301 ccattctgac gctgtatgtc cgcgaactgg cgggtaacgt cagtaacgtc gcctttatca
     6361 gtggcatgat cgcctcggtg ccaggcgtgg cggctctgct gagtgcacca cgactcggca
     6421 aacttggcga tcgaatcgga cccgaaaaga tcctgattac ggcgctgatc ttttctgtac
     6481 tgctattgat cccaatgtct tacgttcaga cgccattaca acttgggatt ttacgttttt
     6541 tgctcggtgc cgccgatggt gcactactcc ccgccgtaca gacactgttg gtttacaact
     6601 cgagcaacca aatcgccggg cgtatcttca gctataacca gtcgtttcgt gatattggaa
     6661 acgttaccgg accattgatg ggagccgcga tttcagcgaa ctacggtttc agagcggtat
     6721 ttctcgtcac cgctggcgta gtgttattca acgcagtcta ttcatggaac agtctacgtc
     6781 gtcgtcgaat accccaggta tcgaactgat ttttcgcctt tcatacttgc aaaagcggag
     6841 aatcagctat ccttttccct gaaacctcat caactcaaag ggagaatcgt gatgaccatg
     6901 tacgcaacgc ttgaagaagc cattgacgct gcacgcgaag aatttcttgc agacaacccc
     6961 agcatcgacg ccgaaaatgc gaatgtgcaa cagttcaatg cccaaaaata cgttttgcag
     7021 gacggcgaca tcatgtggca agttgagttt tttgccgacg aaggggaaga aggtgaatgt
     7081 ttacctatgc ttagcggtga agccgcgcaa agtgtttttg atggcgacta tgatgagata
     7141 gagatccgcc aggagtggca ggaagagaat acattacatg aatgggacga gggggaattt
     7201 cagcttgagc caccgctgga taccgaggaa ggacgcgcag cagctgatga gtgggatgaa
     7261 cgttaatcac tcatacgggc catgatgaat gtcgatcggc agcagtaaag tatcaaaaac
     7321 cagggagaat ggcagatcaa ggatcgtgac ataacgccaa gcggagtcac gcacatccca
     7381 ttgcacaccg ggataagatt ggttgccatg cccctgcccc ggtatagtgc gactaataat
     7441 gctgccacag ccactcagca aggtcaccat gatgctcacc acaattaaac gcatttgata
     7501 cctcttccgt aaaaaaatac cggcataacg ccggtatttt tattggctcg ttgtttttgc
     7561 cagatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa cacattgcag
     7621 gagcgatgta ggcccgataa gcgtagtgca tcaggcaact acgtttttat tgcgaagccg
     7681 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
     7741 aagaatatct ctcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
     7801 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttcagcgcct
     7861 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcccggtga cgcgcggtcg
     7921 ccattgcggt tgcgagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
     7981 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
     8041 gtgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg
     8101 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggagcca
     8161 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccctaaca
     8221 gcagctgtga gccgtggcgt ttgaacgctt ccccccagga agtggagtca tcatcacgct
     8281 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacgacgaag accgtatgga
     8341 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
     8401 taacgcgcca gaagccgcca tattctttcg ttcctttgca ccaaataagc aaaatgctca
     8461 atagctttgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     8521 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     8581 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     8641 cggagagata agacatcacg cccgtcagaa ataccgcacg gtgaaccggg tgcataccct
     8701 tcaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     8761 gctcatcaag caagttaggc ggtaattctt cataagaacc cgggagatcg taagcaatcc
     8821 agactcccca acctgcacgg cgcatcaacg ctgcttccac gaagtcatgt gacaggattg
     8881 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaacggtt
     8941 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     9001 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcatata
     9061 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     9121 ccatcaggcg caccaggccg cacaaacaat caccggtcat taccgagtca gcatccagca
     9181 ccaccatgta gctgtactgg ctgccccatc gacggcagaa gtcatcgata ttaccgcttt
     9241 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcgg
     9301 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     9361 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     9421 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     9481 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     9541 cgcgaccaat aagcagttgc aggaacccca tcaacgccgt ccagaatccg gcggacaccc
     9601 aacagaacag taccgcaaag aggatcagga taccggtttg cagcatataa ggcagaagct
     9661 gcataaagga aacccacaca tcctgaccaa ccatatccat aggattaatc agcgcccacc
     9721 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtaa
     9781 ggattaacaa aatgtaacgg cggatggtac cgacggtacg ccacttttgc tcgctttcct
     9841 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcagacgat
     9901 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     9961 cttctggcat cgctttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
    10021 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggagtcat
    10081 cctcccgcgc ccaggtacgg tggtcggcat ccagcgcctg atgaacggcg cggatatcag
    10141 tcttcggcaa tgccgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
    10201 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
    10261 accagcgcag cacgcatttc agtggttttc ttggcatctt tcactttcac acgcatcacc
    10321 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
    10381 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
    10441 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
    10501 ttcacatccc ccgttgaacg acgcgtttgt tgtacccatg cgttatctgg cgcatgcagt
    10561 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
    10621 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
    10681 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
    10741 ggacgaagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg accgcgctgc
    10801 aacagaccaa agccttgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
    10861 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagagag accgttagag
    10921 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
    10981 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
    11041 tgcacatcca caaccgtgtc acgccctggc ataactacga atttgtaagc acctgtcgcg
    11101 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
    11161 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaggcggt atcaattgcc
    11221 aggccgcgtg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
    11281 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
    11341 aaaccggcaa aaccaagatc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
    11401 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
    11461 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
    11521 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
    11581 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
    11641 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
    11701 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
    11761 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
    11821 ccattccgac aacagaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
    11881 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
    11941 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
    12001 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
    12061 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
    12121 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
    12181 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
    12241 ggcatgtgaa tagcgccgaa ccgtcattgt ggctgaccct ttttaatgac ttcatccact
    12301 cgttccgcat gcaggtattt ttcgttatat ccgggtactt ttcctacatg ctttttttac
    12361 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
    12421 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatacgtc aaagggaaag
    12481 cggaaagttg gcctggcctg tcattgtatg acaaatataa tacgttggcc tgggaattaa
    12541 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgc gtatggatat
    12601 ttaaacgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
    12661 taaaactatc ggtgattttt ttgtgcctcg gcatcggcta tgcggtaata agaagaacga
    12721 tttttattgt gtatccgccc attctgagta acggcatgtt caattttatt gtcatgcaaa
    12781 cgctatttta tttgccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
    12841 aaaccttgtt taccacgccg tctcgtggct gtacccttgc agcagcattg gcatttgtcg
    12901 cttacttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
    12961 tgatcaccat ggtcctcggt ctgtggatgg tgaatgtggt cttctccttc ggccaccgtt
    13021 tgcttaactt ccagtcagcg cgggtgactt actttgttaa tgcatcgctg tttatctatc
    13081 tggttcacca cccgttaact ctgtttttcg gcgcgtacat tacaccgcac atcacatcca
    13141 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
    13201 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
    13261 gtgagaacga taaagcacca gcccgttaag ccgcgtttat aataaccatt ccacgggcaa
    13321 tatcgatgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag
    13381 aacaatttct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
    13441 gagctgccag gccgcatcat actggctctg aataaagcgt ttatcaatta actgtgccag
    13501 cgtttcgctc tctatcacga accccatttc agtattgagc aatgtcgaac gcggatcgaa
    13561 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaggttttag catgcaggct
    13621 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctactttgtt cacgcgtcgg
    13681 cttgagttca tataattcca cgccgtagcg gagcaatttt ttgcgccagc gtgcgtatcc
    13741 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
    13801 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
    13861 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
    13921 cagtgaatgc cgttttgcct tgccttcccc tttcgccgga tcatcactta ataaacgtgt
    13981 tttcgcccag ataagcggca acgttccatc aaccagatga tttataaacg ggctggattc
    14041 cattttgcgt aaataacgat gcgtcatggc atcgttgtgc caggatgctg gtaactcgat
    14101 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
    14161 gcaataccag tagcgggcga aatcatcggc aacgtcctct acaactggtc ctattgccat
    14221 gacatctaaa tccgaaaaaa gtggctcttc ccctgcccca aaataggcat caccaatatt
    14281 tcgcccccct accagcgtca ccacgccatc gacagtgaaa cttttattgt gcatacggcg
    14341 attgagacgg gaaaagtcgg tgatataacc aagcggacgt agcaagcgaa acgagaaagg
    14401 attaaaaagc cggacttcaa tgcggggatg actgtcaagc aagcgtaaaa tgtcgtcaag
    14461 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
    14521 taacagggcg gaaaagagta accgacccga catatcgtcc tgccagatgt aatactgaac
    14581 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
    14641 tttctccaga ggaaaaaggc cacactgacc gggatgttgc gaacacagtg gcagcaccgc
    14701 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
    14761 gggtgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
    14821 taaattctga aacggttttt actgcgattt cagccgctgc cgcacgaggg taaccataaa
    14881 ccccagtact gattgcagga aatgccactg acgtatagct gtttgccgcc accagtcgca
    14941 gactattgag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga
    15001 ctggcccgac ggtgtgcact acggctttag cggaaagatc gcctgcaagc gtaataacgg
    15061 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcagag
    15121 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
    15181 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
    15241 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
    15301 tggttaaatc gcatttttct gctcagcaag gtgagttgtt cagaaactta gttatattcg
    15361 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
    15421 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg aataggttct gcccggaaca
    15481 gagccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
    15541 ggttccacaa tttggccata aaagtggatc acgccccctt gcgcgctgct tcctgcgatg
    15601 gcaataaaag gaaaaaacat actgccgagc attagagagt ctaaaaaagg tctgaacatt
    15661 tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt
    15721 ggctgatccg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
    15781 taagactttt ctgaagaggg cggccattgt tgtgataaat gaagtgactg tccatcagaa
    15841 acagtaacaa ctattttcac ccgatcgtcc ggggaaatat ttaaactcaa cttagtcaaa
    15901 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
    15961 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagtg
    16021 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctgactg
    16081 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
    16141 gcgccctgtt tctttaatac agaggatgta ttagtactga tgagcggtcg cgttgttacc
    16201 aaagccaacc tgagtgacgt taacgctgga gttagatgca gtctggtcaa cagcagcacc
    16261 gttaccgcca ccgaactgtt taacagtcat agtagaatct ttaccattcc actgatcaag
    16321 agtagcgctg ttaccgaagc cacgctgagt cagatcgatt gagctgtcat cagaaccttg
    16381 gccaacatct gcgccgttac cgccgccgtg ctgggtaatg gtcagatcag agttacgggc
    16441 gtcagcttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
    16501 atttgggccg ctattattac cgccaccacc gtggttaccg ccgccgccgt actgaggaac
    16561 aacacctgcc agagcgctac cggagaatac gattgctgca attgctgcta cttttaaaag
    16621 tttcatgtaa aacccccatc ggattgattt aaaagtcgaa tagtaattaa cgttgtgtca
    16681 cgcgaattgc catttgcgac tgtctctgca ctacaattgc cgttttttga gtaccatact
    16741 gtgtaatatt tgctttatta ccagaacctt tctggataat catcgcagta ttaccataag
    16801 caccttgcga aatactggca tcgttggcac tgcccgcctg atcaatatat gcaaggttat
    16861 aatctcctgt ctggtcaatc tttgcccgat tgctactacc ttcttgcgca acaaccgcca
    16921 aaagttttga gcctccctgc cgtaactgag cactattatt agtcccagct tgaccaatta
    16981 tggctgcctg attaaatgaa gacttactca attcatttac cgcgaagtta tattctgaat
    17041 tagctaaatc ataacctgct gcggctgcaa tcccaggcgc acccagtatt gttaacatca
    17101 taaataacaa tttgtttttc atgttgtcac cctggacctg gtcgtacact taagaaatta
    17161 aatcatttca acttggttgt taacgcaacc tgtattttgt taacgctgcg ttacgatgga
    17221 aagtatgtct gcggaaatat ttttaataac tcacccgcgc gttgtatttt tctttttttg
    17281 ttcataccaa agtattaaaa cccacgtaaa tacgctgtta tctacgcaaa aaaatatttt
    17341 gttttctttt aaatctccgt tttccgcaat caaaaatcac cagacagcca ttcttcttgc
    17401 ccgtcgctga ttgctgcgat atgtcttgcg cacaagccgt gacaaacctc gtctttttgc
    17461 agtaaaaaat tgtccacgga ggtgtgaaga aaaaacaaga acgttttaca tgacgaaagg
    17521 actacaccga aatatttttt atatgcatta ttagtaagtt agcaccattt gtatgatttt
    17581 ttaaaattgt gcaataaaaa ccaaatgtac aacttttcta tcatttctaa acttaataaa
    17641 acctta
//
LOCUS       DAYPHN010000100        17577 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000100 DAYPHN010000000
VERSION     DAYPHN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.100"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..607
                     /locus_tag="ACG0BW_003124"
     CDS             <1..607
                     /locus_tag="ACG0BW_003124"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HFK7260054.1"
                     /translation="ENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQRLGEW
                     LMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADD
                     VICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAK
                     ISGNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            696..2366
                     /gene="menD"
                     /locus_tag="ACG0BW_003125"
     CDS             696..2366
                     /gene="menD"
                     /locus_tag="ACG0BW_003125"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFK7260055.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVTLWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKAISEL
                     QQAQIVVQLGSSLTGKRLLQWQAICEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            2363..3121
                     /gene="menH"
                     /locus_tag="ACG0BW_003126"
     CDS             2363..3121
                     /gene="menH"
                     /locus_tag="ACG0BW_003126"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFK7260056.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFTSLNDDQ
                     RRELVALRSNNNGTTLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            3136..3993
                     /gene="menB"
                     /locus_tag="ACG0BW_003127"
     CDS             3136..3993
                     /gene="menB"
                     /locus_tag="ACG0BW_003127"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFK7260057.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSADGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            3993..4955
                     /gene="menC"
                     /locus_tag="ACG0BW_003128"
     CDS             3993..4955
                     /gene="menC"
                     /locus_tag="ACG0BW_003128"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFK7260058.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDALPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFTFVAEEGVRAVVIKPTLTGSLEKVREQVEAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPETIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            4952..6307
                     /gene="menE"
                     /locus_tag="ACG0BW_003129"
     CDS             4952..6307
                     /gene="menE"
                     /locus_tag="ACG0BW_003129"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFK7260059.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLDQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQLVPLVNDEGWCAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            6417..6683
                     /gene="pmrD"
                     /locus_tag="ACG0BW_003130"
     CDS             6417..6683
                     /gene="pmrD"
                     /locus_tag="ACG0BW_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFK7260060.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(6677..7063)
                     /gene="arnF"
                     /locus_tag="ACG0BW_003131"
     CDS             complement(6677..7063)
                     /gene="arnF"
                     /locus_tag="ACG0BW_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFK7260061.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMILPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7063..7398)
                     /gene="arnE"
                     /locus_tag="ACG0BW_003132"
     CDS             complement(7063..7398)
                     /gene="arnE"
                     /locus_tag="ACG0BW_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFK7260062.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(7395..9047)
                     /gene="arnT"
                     /locus_tag="ACG0BW_003133"
     CDS             complement(7395..9047)
                     /gene="arnT"
                     /locus_tag="ACG0BW_003133"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFK7260063.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(9047..9937)
                     /gene="arnD"
                     /locus_tag="ACG0BW_003134"
     CDS             complement(9047..9937)
                     /gene="arnD"
                     /locus_tag="ACG0BW_003134"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFK7260064.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPEN
                     MGRHLWRLVKPRFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMVDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGKPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGMPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(9934..11916)
                     /gene="arnA"
                     /locus_tag="ACG0BW_003135"
     CDS             complement(9934..11916)
                     /gene="arnA"
                     /locus_tag="ACG0BW_003135"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFK7260065.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPEDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHSHAPAAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPDNEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(11916..12884)
                     /gene="arnC"
                     /locus_tag="ACG0BW_003136"
     CDS             complement(11916..12884)
                     /gene="arnC"
                     /locus_tag="ACG0BW_003136"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFK7260066.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(12888..14027)
                     /gene="arnB"
                     /locus_tag="ACG0BW_003137"
     CDS             complement(12888..14027)
                     /gene="arnB"
                     /locus_tag="ACG0BW_003137"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFK7260067.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARLLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVNEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTMSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            14335..14937
                     /gene="ais"
                     /locus_tag="ACG0BW_003138"
     CDS             14335..14937
                     /gene="ais"
                     /locus_tag="ACG0BW_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFK7260068.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(14976..15401)
                     /gene="nudI"
                     /locus_tag="ACG0BW_003139"
     CDS             complement(14976..15401)
                     /gene="nudI"
                     /locus_tag="ACG0BW_003139"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFK7260069.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLTFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            15680..16222
                     /gene="yfaZ"
                     /locus_tag="ACG0BW_003140"
     CDS             15680..16222
                     /gene="yfaZ"
                     /locus_tag="ACG0BW_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFK7260070.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            16322..17530
                     /gene="yfaY"
                     /locus_tag="ACG0BW_003141"
     CDS             16322..17530
                     /gene="yfaY"
                     /locus_tag="ACG0BW_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFK7260071.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMTPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSSAL"
     gene            complement(17514..17577)
                     /locus_tag="ACG0BW_003142"
                     /pseudo
     CDS             complement(17514..17577)
                     /locus_tag="ACG0BW_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4227 a         4480 c         4729 g         4141 t
ORIGIN      
        1 cgaaaatgct tttcttggct cttcaccgga acgattatgg cggcggcgtg acaaagcgct
       61 gcgtactgaa gcgctggcgg gaacggtagc aaatcatcct gatgataagc aggcgcagcg
      121 gttgggtgag tggctgatgg cggatgataa aaaccagcgc gagaacatgt tggtggtaga
      181 ggatatctgc caacgattgc aggccgatac ccagacgttg gatgttttac cgccgcaggt
      241 actgcgtctg cgtaaagtgc agcatcttcg ccgctgtatc tggactgcac tcaacaaagc
      301 ggatgatgtg atctgtttac atcagttgca gccaacggca gcagttgctg gcttaccgcg
      361 cgatctggcg cgacagttta tcgcccgtca cgaaccgttc acccgagaat ggtacgccgg
      421 ttctgcgggc tatctctctt tacaacaaag cgaattctgc gtttccctgc gctcagcaaa
      481 aatcagtggc aatgtcgtgc gattatatgc tggcgcgggc attgtccgtg gttccgaccc
      541 cgagcaagag tggcaggaaa tcgacaacaa agcggcaggg ctgcgtactt tattacaaat
      601 ggaatagtaa tgagtcgcat cattaccgat tcatatcaat aatctatttt tgtagctcct
      661 tatacttagc cgcaatattg ataccggaca aactcatgtc agtaagcgca tttaaccgac
      721 gctgggcggc ggtcattctg gaagcattaa cgcgtcacgg cgtcagacac atctgtatcg
      781 cccctggctc gcgttctaca ccgttaacgt tagcggcggc ggagaattcc gcattcattc
      841 accacaccca tttcgatgag cgtggactgg ggcatctggc gctggggctg gcgaaagtca
      901 gcaagcagcc ggtggcggtg attgtgacct ccggcacggc ggtagcaaat ctctatcctg
      961 cactgattga agctgggtta accggagaaa aactgatcct gttaaccgcc gatcgcccgc
     1021 cggagctaat tgactgcggc gcgaatcagg cgattcgtca gccgggaatg ttcgcctctc
     1081 accccacgca cagtatttca ctgccgcgcc cgacccagga tatccccgca cgttggctgg
     1141 tttctaccat cgaccatgct ctcggtacgc ttcatgctgg tggagtccat atcaactgcc
     1201 cgtttgctga accgctgtat ggcgaaatgg acgacaccgg gcttagctgg caacagcgtc
     1261 tgggcgactg gtggcaggac gataagccgt ggctgcgtga agcgcctcgt ctggaaagtg
     1321 aaaaacagcg cgactggttc ttctggcgac aaaagcgcgg cgtggtggtt gccgggcgca
     1381 tgagtgcgga agagggcaaa aaagttacac tgtgggcgca aactcttggc tggccgctga
     1441 tcggcgatgt gctgtcgcaa accgggcagc cgctgccgtg tgccgatctc tggttaggca
     1501 atgccaaagc gatcagcgaa ctgcaacagg cgcaaattgt ggtgcaactg ggaagcagcc
     1561 tgacgggcaa acggctcctg caatggcagg caatctgtga acccgaagag tactggattg
     1621 ttgatgacat tgaagggcga cttgatccgg cacaccatcg cggacgtcgc ttaattgcca
     1681 atattgccga ctggctggag caacatccgg cagaaaaacg ccagccctgg tgcgttgaaa
     1741 tcccgcgcct ggctgaacag gcaatgcagg cggttattgc ccgtcgcgat gcgtttggcg
     1801 aagcgcaact ggcgcatcgc atcagcgact atctgcctga acaggggcaa ttgtttgtcg
     1861 gtaacagcct ggtggtacgt ctgattgatg cgctttcgca acttccggca ggttacccgg
     1921 tgtacagcaa ccgtggtgcc agcggtatcg acgggctgct ctcaactgcc gctggcgttc
     1981 agcgggccag tggcaaaccg acgctggcga ttgtgggcga tctctcagca ctttacgatc
     2041 tcaacgcgct ggcgttatta cgtcaggttt ccgcgccgct ggtattaatt gtggtgaaca
     2101 acaacggcgg gcaaattttc tcgctgttgc caacgccgaa aaacgagcgc gagcgtttct
     2161 atctgatgcc gcaaaacgtc cattttgagc acgccgccgc gatgttcgag ctgaaatatc
     2221 atcgtccgca aaactggcag gaacttgaaa cggcgcttgc tgacgcctgg cgcacgccaa
     2281 ccaccacggt gattgaaatg gtggttaacg acaccgatgg tgcgcaaacg ctccagcaac
     2341 ttctggcgca ggtaagccat ttatgatcct gcacgcgcag gcaaaacacg gcaaaccagg
     2401 tttaccctgg ctggtgtttt tgcacggttt ttccggcgat tgccacgaat ggcaagaagt
     2461 gggcgaggcg tttgccgact actcgcggtt gtatgttgat ctcccaggtc acggtggttc
     2521 agcgacgatc agcgtcgatg gatttgatga tgtcaccgac ttactgtgta aaacgctggt
     2581 tagttacaac atccttaact tctggctggt ggggtactcg cttggcggac gggtggcgat
     2641 gatggcggct tgccaggagc tggcggggct ttgtggagtt gttgtcgaag gcgggcatcc
     2701 tgggttgcaa aatgctgaac aacgcgcgga acgtcagcgt tccgatcgcc aatgggcgca
     2761 gcgttttcgt acagaaccgt taacggcggt atttgccgat tggtatcaac agcctgtttt
     2821 tacctcgctc aatgacgatc aacgccggga gctggtggcg ctgcgcagca acaataatgg
     2881 cacaaccctt gccgccatgc tggaggcgac ttctctcgcc gttcagcctg atttacgtgc
     2941 taaccttagc gcccgcacat ttgcgtttta ttatttatgt ggtgaacgtg acagcaaatt
     3001 ccgcgccctg gcggcggaac tggctgccga atgccatgtc attcctcgcg ccggacataa
     3061 cgcgcatcgg gaaaatcccg ctggcgtaat cgcaagtctg gcgcagatct tgcgtttctg
     3121 actaaaggac acaatatgat ttatcctgat gaagcaatgc tttacgcacc ggttgaatgg
     3181 cacgactgct ccgaaggttt cgaggacatt cgttatgaaa aatctgccga cggtatcgca
     3241 aaaatcacca ttaatcgtcc gcaggtgcgc aatgccttcc gtcctctgac ggtaaaagag
     3301 atgatccagg cgctggcaga tgcgcgttat gacgacaaca tcggcgtgat cattctgact
     3361 ggtgcaggcg ataaagcgtt ctgctccggt ggtgaccaga aagtgcgtgg cgattacggc
     3421 ggctataaag atgattccgg cgtacatcac ctgaacgtgc tggacttcca gcgtcagatc
     3481 cgtacctgtc cgaaaccggt tgtcgcgatg gtggctggct actccatcgg cggcggtcac
     3541 gttctgcaca tgatgtgcga cctgactatc gcggcagata atgccatctt cggtcagact
     3601 ggcccgaaag tcggttcctt cgacggcggc tggggcgctt cctacatggc gcgcatcgtc
     3661 gggcagaaaa aagcacgtga aatctggttc ctgtgccgtc agtacgacgc gaaacaggcg
     3721 ctggatatgg gccttgtaaa caccgtggta ccgctggcgg atctggaaaa agaaaccgtc
     3781 cgctggtgcc gcgaaatgct gcaaaacagc ccgatggcgc tgcgctgcct gaaagctgca
     3841 ctgaacgccg actgtgacgg tcaggcaggt ctgcaggagc tggcgggcaa cgccaccatg
     3901 ctgttctaca tgacggaaga aggtcaggaa ggtcgcaacg ccttcaacca gaaacgtcag
     3961 cctgacttca gcaaattcaa acggaatccg taatgcgtag cgcgcaggta taccgctggc
     4021 agatccccat ggacgcgggg gtggttctgc gcgacaggcg gttaaaaacc cgcgacgggc
     4081 tgtacgtttg cctgcgtgag ggcgagcgcg aagggtgggg ggagatctcc ccactgccgg
     4141 gcttcagcca ggaaacctgg gaagaggcgc aaagcgtgct gcttgcctgg gtaaataact
     4201 ggctggcagg cgattgcgag ctaccgcaga tgccttccgt ggcctttggc gtaagctgtg
     4261 cattggcaga actgaccgat gcgttgccgc aagcagccaa ctaccgtgcg gcaccgctgt
     4321 gtaatggcga tccggacgat ctgatcctca aacttgccga tatgccgggc gagaaagtgg
     4381 cgaaggtcaa agtgggattg tacgaagccg tgcgcgacgg catggtggtc aatctgttgc
     4441 tggaggcaat tccggatctg catttgcgtc ttgacgcgaa ccgcgcctgg acaccgctga
     4501 aaggccagca gtttgccaaa tacgttaacc cggattatcg ccaccgcatt gcttttctcg
     4561 aagagccgtg caaaacccgc gatgattcgc gagcgtttgc ccgtgaaacc ggcattgcca
     4621 ttgcctggga tgaaagcctg cgcgagccgg attttacctt tgtggctgaa gagggcgtgc
     4681 gcgcggtagt tatcaaaccc acgctcacgg gcagcctgga gaaagtacgc gagcaggtag
     4741 aggcggcgca cgcgttgggg ctgacggcgg tgatcagttc atctattgag tcgagcttag
     4801 gcttaacgca actggcgcgg attgccgcct ggttaacgcc agagactatt ccagggctgg
     4861 acacgctgga tctgatgcag gcgcagcagg tacgtcgctg gccgggtagc acgctgcctg
     4921 tcgtggaagt tgatgcgctg gagcggttgt tatgatcttc tctgactggc cgtggcgtca
     4981 ctggcggcaa gtgcggggag aagccatcgc cttacgtctt aatgacgagc aactcaactg
     5041 gcgcgagctt tgtgctcgcg tcgatgaatt agcctccggc tttgcggcgc agggcgttgt
     5101 tgagggcagc ggtgtgatgt tgcgggcgtg gaatacgcca caaacgctgc tcgcctggct
     5161 ggcgttactg caatgcgggg cgcgggtgct gcccgtgaac cctcagctgc cgcaaccgtt
     5221 gcttgaagaa ttgctgccca atcttacgct gcaatttgcg ctggtgccgg atggggaaaa
     5281 cacgtttcca gcattaactt cgctgcacat tcagctggtt gaaggcgcac atgccgctgc
     5341 gtggcagccg acgcgtctgt gctcaatgac gttaacctct ggttccactg gcttgccgaa
     5401 agccgctgtt catacttatc aggctcatct tgccagcgcc gaaggggtgt tatcgctgat
     5461 tccgtttggc gatcacgatg actggttgct ctctttaccg ctgtttcacg tctccggtca
     5521 gggaattatg tggcgctggt tatacgctgg tgcgcggatg acggtgcgtg ataaacagcc
     5581 attggatcaa atgctggcag gctgtactca cgcttcactg gtgccaacac aactctggcg
     5641 tttgctggtt aaccgcagtt ccgtttccct gaaagcggtg ttacttggtg gtgcggcaat
     5701 tccagtcgag ttgacggaac aggcgcgcga gcaggggatt cgttgctttt gcggctatgg
     5761 tctgaccgag tttgcctcca cagtgtgtgc gaaagaagcc gacggtctgg cagacgtcgg
     5821 ttcgccgctg ccgggtcggg aagtgaaaat cgtaaacgac gaagtgtggc tgcgggctgc
     5881 cagcatggca gaaggttact ggcgtaacgg gcaactggtt ccactggtta atgatgaagg
     5941 ctggtgcgcc acgcgcgatc gcggtgagat gcataatggc aagctgacca ttgtcggacg
     6001 tttagacaat ctgttcttca gtggtggcga aggcattcag ccagaagaag tcgagcgcgt
     6061 tattgctgca catcctgcgg ttttgcaggt gtttatcgtt cccgttgctg acaaggagtt
     6121 tggtcatcga ccggtagcgg tggtggagta tgaccagcaa accgttgatc ttggcgaatg
     6181 ggtgaaagat aagctggccc gttttcagca accggtgcgt tggttaacgt tgccgcctga
     6241 actgaaaaac ggcggtatta aaatttcacg tcaggcgcta aaagagtggg tgcaacgtca
     6301 gcaataatta accctccgtt tcacttctca tttctgccat tccattgccg ttgataaaga
     6361 gtttatctgt taatctgtaa attgatgtga aaactcttag caaacaggat aatgcaatgg
     6421 aatggctggt caaaaaatcg tgctgcaata aacaagataa cagacatgta cttatgctct
     6481 gcgatgctgg cggcgcgata aaaatgatcg ccgaagtgaa gagtgacttc gccgtgaaag
     6541 tgggggattt actctcccct ttgcagaatg cgctttattg tattaatcgt gaaaagctgc
     6601 atacggtgaa agtcctctcg gcaagcagtt atagccccga tgagtgggaa cggcagtgta
     6661 cagcggcagg gaaaactcag taacgttgtt ttgtcgtggg caggaaaatc aacatcaacc
     6721 cgctcataat acaagcaact cccagtagtg ccttcagcga aaaagttcct tcccagccgg
     6781 gaagtatcat tgatgcaatc cataccagca catagctcat actgagcaag gcataggctt
     6841 tgcttaacgc aagtttatgt agcgttttat accaacaaaa aacggaaagc agataaccca
     6901 gcagcccgag taacagtatc cgggcatcca ggccaaaagc taacagcgcc gcgataaaat
     6961 cccataagtg cgtcatcggc ggcagatgac tcgccgcaaa acccaggctt aattgcgcag
     7021 cggaggcgat aatgacgctg aataatcccc acatcaggcc cattacacgg tactcccgag
     7081 gatcacaatg ccaccaataa tgaacgccac cccacaccag tgacgcggcg ataccggttc
     7141 gtgccacagt tttactgcag ccagcgtcac ccagacaaaa ttcagactta acatcgggta
     7201 ggcaatgcct accggtacgt tctgcaagac cagcagccag agcaccatgg caagaccaag
     7261 acaagccagc gccagtccca gccacagcac gatatgtttg cgccgtttgt ttatcgccac
     7321 aaagcaggtt gcctgtttct gacacaactg cccggcaacg ctaagcaagc tggcaaagac
     7381 taatgttagc cagatcattt gggacgatac tgaatcagca ccagacgctc ctgacgatcg
     7441 atggcatctg cgggcggaat ggcgagactg ttgatatctt cattgcggtc aaccgaaagc
     7501 acgagtgtaa taatcccctc ctgacgatgt tgattaagcc agtttgcgaa ctcatcaccg
     7561 ctgacaaatc tccctttcgc atccggataa ttaaggccgt atttcaactc acctgtctgg
     7621 cgatacatga tgatgtcatc gcgttgcagg ctccatgcca gacctgcggc aacaccgacg
     7681 ctatcagtaa gaatatagcg gcttggctga agtgattctt gtgtcatctc gacaaaaaat
     7741 tgcggatgtt ttccttccat gactctgtca ggaattgaaa atcctaccag caacgccagc
     7801 cccagcgggc aaagcgcggc aaaagaccag gtcttttcga cgtttgttaa ggtgtaccag
     7861 ccgaaaaatg cccatagcga aaaaatcgac caggcacaaa agactttata gctctcgaag
     7921 gtttgccaca ctggcgtgtt cattggtccc catggggaga ccacaaatgt ggcaataatg
     7981 ccagtgacac caaaagcgat gttaatccag ccattaatcc gcagcgccag aggattattt
     8041 tttgctgcca gcaaagcgta atgcgccatc agcattgcca gaggtgcaaa gcaggaaaga
     8101 atataggtgg gcaatttgcc tttggcgacg gagaaaaaca gcagcggcat tatcgtccag
     8161 ctcaacaaat agacggttgc ggaatgcttg cggtttttcc agcctgcgta cagtgctccg
     8221 gggagtaatc ccagccacgg caggctaccg gcaatgatga ccggcaagta gtaccagaac
     8281 ggagctctat gctgagcatc atccattgca aagcgttgaa tatgctcaac ccagaaaaaa
     8341 tagtgccaga agtcaggctc ccgctgcgct atcgccagtc cccaggggag aaccgtcagt
     8401 acgcaactga tgaccgccag ccagccgtaa ataaagagat ctttccagcg tttttgcgtt
     8461 gcaacccatg gaagcacgct taataccggc acggcaaggg cgagaaaacc tttggtcatc
     8521 acccccatgc cgcaggtgat tcccagcagt aaaaatcctg cgcttttgcc tttccacgtc
     8581 tgtgcctgca ttgccagcca gaagctgcac attcccgcca ccagccagaa ggcaataaac
     8641 ggatcgagca cggcataagt gccgatggca tagacaataa acaatgagag ataaatcact
     8701 gcggcgagta atgccagacg tttatcgcgc cataagcgca gagtaaacca ggtcaccagc
     8761 gcggcagtta acagggtcgc aaagataacg cctgcccgca caccaaagtt attcgcgcca
     8821 aatagccatt gcccaatgct gttaatccag tatccggcaa tgggtttttc gaaataacgt
     8881 agccctaaca gatggggcac aatccagtcg ccggatgcca gcatttcccg actgatttcc
     8941 gcataacgcg tttcatcggg ttgccagagc agacgcgtgc tgatcggtaa caggtaatag
     9001 caggcaataa acgcgaagag gccgataagg taacgtaccg atttcatcag cgactacccg
     9061 caatttgttg gcaacccagc cagccttcac gtccggcaat atttccgcga acaacttgcc
     9121 cgagcggcaa cgtccctgat aacagttcgc ttaaagggca aaatgtcacg ccttcctggg
     9181 ctgcccgttt caggagatcc acaaaattat gctgataagc gcagccttca acttctgcat
     9241 gaatggtata aaccggcatg cctttatctc gcaggatgcg gttgagtaac caaccgttaa
     9301 aatcttctgc tttcacatcc cgaccaatca cttcatccca ggtgggtaag gtcaccggaa
     9361 tttgcgcagt accgggtttt cctgattcga gcaatggacg aaacggcatc gccccacgac
     9421 aatcgctgtt gtagcgcaaa tggaacgctt cttttgcttc gatcacctgc tggtcggcac
     9481 gccagccagc ggctgcggag caagttaccg gttggccgat aatttcttcc agggtgcgaa
     9541 ggccgcgtgc gatatcgtcg accattgttt gccgatccca gttaccgcta tgggcttgcc
     9601 atgcatggtg atcccaggcg tgcaggccga cttcgtggtg tttagccgct tcacgaatga
     9661 tatcggcatt ggcatgacca atctctttac ctggccaggc cgtacctgcc agtaaaatat
     9721 cccagccata aagcgatgcc gcgtttgagc gcagcatctt ccacaaaaac cttggcttca
     9781 ccagtcgcca gagatggcgg cccatatttt ccgggccgac gctgaaaaaa atgctggcct
     9841 gaatattatg cttactcaag atttccagca gacgcggcac gccttcacgg gtgccacgaa
     9901 aggtatcgac atcaatgcgt aagccgactt tggtcatgat ggtttatccg taagatcaac
     9961 ggtgcgcagg aagaaatcca gcgtttcgtc gatggtttcc tgcatatcaa tttttggttc
    10021 ccagtccagg cagcggcggg cattgcggat gctcggttta cgatgctcta catcctgata
    10081 tcctttgccg tagtagctgc tactttcgac aacacgaaag cccgcaaacg gtgggaagtg
    10141 atggcgcagc ggatgttttt cgaagctcgc cagcagcatc tcgcccagtt cctcaatgct
    10201 cgcttcgtta tcaggattgc caatgttgat aatttcgccg tcgcagcgat ttcccgcatt
    10261 ttcgataatg cggtataacg cctcgatacc atcgcgaata tcagtaaagc agcgtttttg
    10321 ttttccgcca tcaatcagct taatcggtga accctctacc agattgagaa tgagttgcgt
    10381 aatagcgcgg gagctgccga ttcgtgccgc attaaggtta tccagtcgtg gtcccatcca
    10441 gttaaacggg cggaagaggg tgaactgtaa accctctttt tcgccatagg cccagataac
    10501 ccgatcaagt aattgtttag acaccgaata aatccagcgt ggtttattca ccgggccgac
    10561 gattaaatta gaatgatcct catcgaagta tttatcgcta cacatcccat aaacttctga
    10621 agtcgacggg aagatgattc gcttacggta cttcacgcag tagcggataa tgcgcagatt
    10681 ctcttcaaaa tcgagttcaa atacgcgcag tgggttgcgg gtatattcaa tcggcgtggc
    10741 tatcgccacc agcggcaaga cgacatcaca ttttttgacg tgatactcaa tccattcgga
    10801 atgaatactg atatcgcctt cgacaaagtg aaaatgcgga tgattcagaa aacggcttat
    10861 cgcatcgctg ccgatatcca gaccgtaaac ttcataatga tcttcgcgca gcaggcgttc
    10921 tgtcagatgg ttgccaataa agccattcac cccgaggatg agtacccggg tacggcgtcg
    10981 ggcggcacag gcaggctggc tattcaagcg tgaaccttgc accaggccca gcgtctgcgc
    11041 taattgcgag ccctgcatag taatgccgtc gcccgcctgt ccggtgacga tttccagcgc
    11101 gccatcgcca caggcaatca gcagtggcgc aacagaaatc acgctccccg gttgtgctgc
    11161 gggcgcatga gaatgcacgc gtgacgacca gacggtaaat ttctgattac caacatagct
    11221 gaaggcaccc ggccacggat cggcaacggc acgcaccatg ttgtgcagca cggatgccgg
    11281 tttatgccat tcgaggaaac tgtcttccgg tgttctgcga ccaaaacagg tggcttcgtt
    11341 ttcgcgctgg gcgatttcca gaatattacc gtgtttaatg gcgggtaatg tctgttccag
    11401 tagctggcgc gcggcatggc acaatttatg atgcagcgta atagcgatat cgtctggcgc
    11461 aatggcaatg cgcagttgcg ccacaatggc cccggcatcg gcacgtttca ccattcggtg
    11521 caatgtaacg ccagtttccg tttcaccgtt caccagcacc cagttcagcg gcgcgcgacc
    11581 acgatatttt ggtaacagcg aaccatgcag attaaatgca cctgcgggag cgagctgcaa
    11641 aatctcgtcg caaataagat ggcgataata aaaagagaaa atcacctctg gtgagagttg
    11701 ggcaatacgt tccacccaca gcggatgatt aacgttatcc ggcgcataaa ccggaatgcc
    11761 tctttccgcc gccagacgag ctacggaacc ataaaaggct ttttcaccgg gattatcggt
    11821 atgggtaaaa atggcgctaa tttcgtaacc ggcagccagc agggcttcaa taccgaggca
    11881 tcccatatcg tggtaggcaa aaacgacggt tttcattatt cattttcctt gctggatgga
    11941 cggataactt gctgaacaaa atagcggggg cgggcgcgga catcggtgta gatcctgccg
    12001 atatattcac cgagtaatcc catgccgata aactgagcgc caataaaagt aaacagcacg
    12061 gcaaatagca taaagacgcc ttctgccgcc cattgtggtc cgaaggtcag gcgtaaaatc
    12121 accagcagca cagcaatgct aaaacctcca atcgcaataa tgctgccaag cagactcagc
    12181 atacgtagcg gcgttgtggt aaggcaggtc accaggtcgt acatcaaatt aatcaggcgc
    12241 ataaaactgt atttggattc accaaactca cgctcggcat gatgcacggg aatttcaatg
    12301 gcacggcggg cgaagatatt cgccagaatc gggataaagg tactgcgttc atggcagtgc
    12361 aacatcgcat cgacaatatg acggcgatag gcgcgcagca tacaaccgta gtcacccatc
    12421 gctttgccgg tggtgcgctg aataagccgg ttaatcatct tcgatgccgt tttacgaaac
    12481 cagctgtcct ggcggttctg acgcacagtt cctaccacgt cgtagccttc atcggctttt
    12541 gccaccaggc gggggatctc ctctggcgga ttctggagat cggcatcaag ggtaataatt
    12601 aagtcgccag taacgtgact gaatcccgcc ataatcgctg aatgttgccc gtaattgcgg
    12661 ttaagcaaaa tagacacaat atggctgttc tccgcttgtg aggcttcgac cagcatatgc
    12721 gcggaattat cgctactgcc gtcatcaatc agcaggatct catactcttt ccccagtgat
    12781 tcacaggctt ttgtggtgcg cctgattaat tccggtaagc tttcctgctc gttataaacg
    12841 ggaataacca ccgagacttt cttaacaggg tggatttcaa acatggctta ttgtcctgcg
    12901 agttgctgaa gggctgtgat gacgcggtcg gcatcggcgg tggtcatatc cgggaacagc
    12961 ggcaacgaac agatgcgttc gctattccat tcggtattcg gtaacgacat cgtgggaaaa
    13021 cgctcgcgat aatatttttg tgtgtgagcg gcgcggaaat gtaacccggt accaatgcct
    13081 ctttctttta acgcttccat caacgcatcg cgactgatac cacaacgttg ttcattgaca
    13141 cgaataataa acagatgcca ggcgtgaacg tgcggccagg cgggaaggct taatggctga
    13201 aaggggagag ctgccagtgc ttgctgatat tgcagagcaa tttcgcgccg acgggtgttg
    13261 aggtgctcta atttggctaa ctgggtcagg gcaatcgcgg cgttaatatc ggtcagatta
    13321 tacttatagc ccggcgttaa gacttcagct tgcggtgcac ggccccaggt ttgtctgtca
    13381 taggcatcga cacccagacc gtgaaatttc agcatccgta gcaggcgggc aaggttttca
    13441 ttatctgtaa caatcaggcc accttccgca caggtaatat ttttgatggc gtgaaacgaa
    13501 aaaatagcgg taccttttgc gccaatatgt cgccctttgt aatacgtacc gacggcatgg
    13561 gcagcatctt cgataactgc gatgccgtaa cgttcgccaa tggcgcgaat ggcgtcaata
    13621 tctgctggcg caccggcata atgcaccgga atgatggctt tagtgcgtgg cgtaatggct
    13681 gactcgatcg cttcaggcgt gaccatcagc gtatcgcggt cgacatccac cattaccggc
    13741 gttgcaccca acaaggaaat catattgagg gttgaaaccc aggtcaggga aggcgtaatc
    13801 acttcatcgc ccttgccaat ttccaacgcc attagcgtga tatgcattcc ggcggtggct
    13861 gaactgaccg cgatggcatg ctgatttccc gtcaactggc aaaaggcttg ttcaagcgcc
    13921 tgattcttcg gaccggttgt gatccaaccg gattcgagaa cctctttaac tgcagcgagt
    13981 tcctccacgc ccattgctgg tcgcgaaaaa ggcaaaaatt ctgacattgc ttttccttcc
    14041 gccattgaat acctgtccac ttatatttgc tataaagagt gttgtgtata ttttgccatt
    14101 tggagcaaaa ttttaaggat agaatattaa cttaacctta agaaactaat attagacgta
    14161 aatattgaaa tttttatatt ttttcttatt taggctttgc atttggcaaa attttgaggc
    14221 attttgccgg catcgcagga tttttaatat tacaccaact gctaattatc gccagaaatg
    14281 tcgctcaatt tcacggtaat tgtctggttg cgcttgtcta taggtggagt ttacgtgtta
    14341 gctttttgcc gctcttcgtt gaagtcaaaa aaatatttca tcattttact ggcgctcgct
    14401 gcaattgccg gactgggtac tcatgccgcc tggagtagca atggtttgcc acgtatcgac
    14461 aataaaacgc tggccagact ggcacagcag catccggttg tcgttttgtt tcgccatgct
    14521 gaacgttgcg atcgttcaac caatcaatgc ttgtcagata aaacaggtat tacggtcaaa
    14581 ggtacccagg atgcccgtga actgggcaac gcttttagtg ctgatatccc tgatttcgat
    14641 ctttattcca gtaataccgt ccggaccatt cagtcggcga cctggttttc agcgggtaaa
    14701 aagctgacgg tagataaacg acttcttcag tgcggtaatg agatttatag tgcaattaag
    14761 gacttacaaa gcaaagcgcc tgataaaaat atcgttattt tcacccataa tcattgcctg
    14821 acatatattg ctaaagataa gcgtgatgcg acatttaaac ctgattatct ggatggttta
    14881 gtcatgcatg tggaaaaagg caaagtttat ctggatgggg aattcgttaa ccactaaatt
    14941 ttcaatctga cagccagtaa tggctgtcat cattattaca gaagaccttt caaacgtaac
    15001 gtttttcggg tggcgacatt caaatcataa tgcaccagat cttcaggttt tacccacgcg
    15061 tagtcctgaa actcttcgtt tattttcact tctcggttgg cagaaacgca gtcaaaagtc
    15121 aggtaaatca tataaatctc ttccttgcga ccatctgcat acgtcttggt gcgaatatca
    15181 tcgctgaagg tccacggcgt gatttctgtc aaaagcagct gttctcccag ttcttcgcga
    15241 atttcgcggc gtagtgcctc ttcaattcgt tcgccaggct ccacgccacc acccgaaagc
    15301 gcccattgac cggggaaaac gccgcgatcg tcggccattt tacacagcaa ataagcacca
    15361 tcattttgaa tcaaagggca tacaatagtc cgttgtcgca cggggatatt ccttataaat
    15421 aaaaagaaac catgagaata cggtaactca gaaatatgtg aaagtgacag taatctcttt
    15481 tgcgatgata actgcactta ttattatttt tgtgtgtatc ggtaaagtgt gtttattttt
    15541 tcggcgttta attatttaag aagtatttag ttacaataag tgactagatc ccttttttcg
    15601 ctcattgaat cctctgtgcc aggcagtaca atcccgcagc tttgggaatt ttgatttatt
    15661 gcttgtggga gaggggaaaa tgaaaaaaat tgcgctagca ggtctggccg gaatgctgtt
    15721 ggtttctgca tcggtcaatg caatgagcat cagcggtcag gcgggtaaag aatacaccaa
    15781 tattggtgtc ggttttggga ctgaatcgac gggcctggcg ttaagcggta actggacaca
    15841 taacgacgac gacggtgacg tcgcgggcgt ggggctgggg ttgaatctgc ctctcgggcc
    15901 gttaatggcg accgttggcg gaaaaggcgt ttacaccaac ccgaattacg gcgatgaagg
    15961 ttatgccgca gcggtaggag gtggtttgca gtggaaaatt ggcaacagct tccgtttgtt
    16021 tggcgagtat tactactctc cggattcgct ctccagcggt attaaaagtt atgaggaagc
    16081 gaatgcgggc gcgcgttaca ccattatgcg tccagtgagt attgaggcgg gctatcgcta
    16141 cctgaacctt tctggtaaag acggtaaccg cgacaacgcc gtggctgacg gcccgtatgt
    16201 tggggttaac gccagtttct gatcccttcc ggcgcggcat tctgtcgcgc cattcagttt
    16261 cttactacgc cttatcttcc tttgcgttat agtgattcga taacaaaagc aggagataac
    16321 catgttaaaa gtggaaatgc tatccaccgg ggatgaagtg ttacacgggc aaatcgttga
    16381 cactaacgct gcctggctgg ccgatttttt ctttcatcag gggttgccat tatctcgccg
    16441 caatacggtg ggagataacc ttgatgactt agtgaccatt cttcgcgaac ggagtcagca
    16501 cgccgatgtg ctgatcgtta acggcgggct ggggccgacc agtgatgatt taagcgcact
    16561 cgccgctgca acagcaaaag gtgaaggcct ggtgctgcat gaggcctggc tcaaagagat
    16621 ggaacgctat ttccacgaac gtggacgagt aatgacaccg agcaaccgta aacaagcgga
    16681 gctacctgcc agtgctgaat ttatcaataa cccggtaggc accgcctgtg gttttgccgt
    16741 gcagcttaat cgttgcctga tgttctttac ccccggcgta ccgtcagaat ttaaggtgat
    16801 ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt tctttaccgc agccgccggt
    16861 ttgtctgcgt ttgactacat ttggtcgttc ggaaagcgat ctggcacaaa gcctggacac
    16921 cctgcaactg ccgccggggg taacaatggg ctatcgctct tctatgccga ttatcgaact
    16981 gaaactcacc ggaccggcaa gcgagaaaca ggcgatggaa aaactgtggc tggacgttaa
    17041 acgtgttgcc ggacagagcg tgattttcga aggtaccgaa ggactgccct cgcagatcag
    17101 ccgcgaattg caaaaccgcc agttcagcct gacgttgagc gagcaattca ccggtggttt
    17161 attggctttg caactttctc gcgcaggtgc tccattgctg gcgtgtgaag tggttccttc
    17221 acaggaggaa accctggcgc aaactgcgca ctggattaca gaacggcggg ccaaccattt
    17281 tgccgggctg gcactggctg tttctggttt cgagaacgaa catctcaact ttgcgctagc
    17341 cacgccagac ggcactttcg ctctgcgtgt gcgtttcagc actacgcgct acagcctggc
    17401 tatccgtcag gaagtgtgcg caatgatggc actgaatatg ctgcgccgtt ggttaaacgg
    17461 ccaggacatc gccagtgagc atggctggat tgaggttgtt gagtccatga ctttatccag
    17521 tgcgctgtaa aaccccgtcc ttcagggcgg ggatataagg cgcggttttc cacctga
//
LOCUS       DAYPHN010000101        17548 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000101 DAYPHN010000000
VERSION     DAYPHN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.101"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            36..1295
                     /gene="mtnK"
                     /locus_tag="ACG0BW_003143"
     CDS             36..1295
                     /gene="mtnK"
                     /locus_tag="ACG0BW_003143"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HFK7260072.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            1305..2420
                     /gene="mtnA"
                     /locus_tag="ACG0BW_003144"
     CDS             1305..2420
                     /gene="mtnA"
                     /locus_tag="ACG0BW_003144"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HFK7260073.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            2451..3092
                     /locus_tag="ACG0BW_003145"
     CDS             2451..3092
                     /locus_tag="ACG0BW_003145"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFK7260074.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            3196..4200
                     /locus_tag="ACG0BW_003146"
     CDS             3196..4200
                     /locus_tag="ACG0BW_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFK7260075.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVVCVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(4241..5227)
                     /gene="yjhU"
                     /locus_tag="ACG0BW_003147"
     CDS             complement(4241..5227)
                     /gene="yjhU"
                     /locus_tag="ACG0BW_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFK7260076.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(5574..6923)
                     /gene="yjhF"
                     /locus_tag="ACG0BW_003148"
     CDS             complement(5574..6923)
                     /gene="yjhF"
                     /locus_tag="ACG0BW_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFK7260077.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            complement(7030..8997)
                     /gene="yjhG"
                     /locus_tag="ACG0BW_003149"
     CDS             complement(7030..8997)
                     /gene="yjhG"
                     /locus_tag="ACG0BW_003149"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HFK7260078.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDL
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTRLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            complement(9008..9913)
                     /gene="yjhH"
                     /locus_tag="ACG0BW_003150"
     CDS             complement(9008..9913)
                     /gene="yjhH"
                     /locus_tag="ACG0BW_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFK7260079.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            complement(9918..10706)
                     /gene="yjhI"
                     /locus_tag="ACG0BW_003151"
     CDS             complement(9918..10706)
                     /gene="yjhI"
                     /locus_tag="ACG0BW_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFK7260080.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVDCGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            complement(11009..11791)
                     /gene="sgcR"
                     /locus_tag="ACG0BW_003152"
     CDS             complement(11009..11791)
                     /gene="sgcR"
                     /locus_tag="ACG0BW_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFK7260081.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IVNQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            complement(11808..12440)
                     /gene="sgcE"
                     /locus_tag="ACG0BW_003153"
     CDS             complement(11808..12440)
                     /gene="sgcE"
                     /locus_tag="ACG0BW_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HFK7260082.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            complement(12452..12883)
                     /gene="sgcA"
                     /locus_tag="ACG0BW_003154"
     CDS             complement(12452..12883)
                     /gene="sgcA"
                     /locus_tag="ACG0BW_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcA family PTS sugar transporter subunit IIA"
                     /protein_id="HFK7260083.1"
                     /translation="MINDIKWVQAQRKATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLTAVGNASTDDALFALVSG"
     gene            complement(13014..13820)
                     /gene="sgcQ"
                     /locus_tag="ACG0BW_003155"
     CDS             complement(13014..13820)
                     /gene="sgcQ"
                     /locus_tag="ACG0BW_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HFK7260084.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            complement(13833..15146)
                     /gene="sgcC"
                     /locus_tag="ACG0BW_003156"
     CDS             complement(13833..15146)
                     /gene="sgcC"
                     /locus_tag="ACG0BW_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC SgcC"
                     /protein_id="HFK7260085.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLLPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            complement(15158..15436)
                     /gene="sgcB"
                     /locus_tag="ACG0BW_003157"
     CDS             complement(15158..15436)
                     /gene="sgcB"
                     /locus_tag="ACG0BW_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB SgcB"
                     /protein_id="HFK7260086.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            complement(15433..16554)
                     /gene="sgcX"
                     /locus_tag="ACG0BW_003158"
     CDS             complement(15433..16554)
                     /gene="sgcX"
                     /locus_tag="ACG0BW_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFK7260087.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTMELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            complement(16799..16915)
                     /locus_tag="ACG0BW_003159"
                     /pseudo
     CDS             complement(16799..16915)
                     /locus_tag="ACG0BW_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(16953..17171)
                     /locus_tag="ACG0BW_003160"
                     /pseudo
     CDS             complement(16953..17171)
                     /locus_tag="ACG0BW_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17340..>17548)
                     /locus_tag="ACG0BW_003161"
     CDS             complement(17340..>17548)
                     /locus_tag="ACG0BW_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFK7260088.1"
                     /translation="VVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAEVRAELN
                     IAPKRYVTYARECFGWGVFALIAR"
BASE COUNT         4395 a         4423 c         4399 g         4331 t
ORIGIN      
        1 ttgattatca ttctgtgtag acaaggagaa ctgttatgac ggattcaatt ccatcaggtt
       61 acaagccgct gacatgtgac acgctgccgg gttatctctc gtccagactg accccttcat
      121 gcgaaccggg agggttacct gaagagtgga aagtttcaga agtgggggac ggaaacctga
      181 acatggtgtt tatcgttgag gggacacata aaaccatcat tgtaaaacaa gctctgccct
      241 ggcttcgtgc cgggggcgaa ggatggcctt tatctctgag ccgtgcgggc tttgagtaca
      301 acgtcttatg tcaggaagcc aagtacgcgg gtcacacact gattccgcag gtctattttt
      361 acgacccgga aatggcgctg tttgccatgg agtatctgac tcctcacgtg attctgcgta
      421 aggaactgat taacggtaaa aaattcccta aactagctga agatatcggc agatttttag
      481 cacagactct cttcaatacg tctgacattg gcatgtcagc agaacagaaa aaagcgctta
      541 ctgccgagtt cgcgttgaat catgagctgt gcaaaattac ggaagatctg atcttcacag
      601 agccctatta caacgctgaa cggaataact ggacttctcc tgagctggac gatgccgtcc
      661 ataaggcctg ggctgatgta gagatgatcc aggttgccat gcgttataag tacaaattta
      721 tgacagaagc gcaggcattg ttacatggcg accttcattc aggctcaatc atggtgaccg
      781 acacggatac caaagtgatt gatccggagt tcggtttcat ggggccaatg gcgtttgata
      841 tcggcaacta tattggcaac ctcctgctgg cgtacttctc acgccctggg tgggatgcga
      901 atgagcaacg tcgcgctgac tatcaggaat ggctgcttca gcagattgtc caaacctggt
      961 ccgttttcac ccgggagttc cgccagctct gggacaacaa aacacagggc gacgcgtggt
     1021 cgacagaaat gtatcaacag aacagggccg ctcttgagga cgcacaggat cagttttttg
     1081 ccacgctgct tgaagattcc ctggtgaatg ccggcatgga aatgaatcgc cggatcattg
     1141 gttttgctgg cgttgccgag ctgaaacaga ttgaaaatac agagcttcgc gcaggatgtg
     1201 aacgacgtgc attgaccatg gcgcgcgatc ttatcgtcaa tgcccgccag tttaaaaata
     1261 tggattccgt catccagtct gcgaaggtta agtaagaggt cattatgaat attaaaggta
     1321 aacactatcg tacggtctgg gtttccgggg atggaaaagc ggtagaaatc atcgatcaga
     1381 ctaagttgcc ttttaagttc gaggtggtgg cgcttacctc tgcggaaatg gccgctacgg
     1441 ctattcagga tatgtgggtt cgtggcgctc cgcttattgg tgtcgttgca gcctatggca
     1501 tcgcgctggg aatgaatcac gatgccagtg acatgggtct gcagcgttac tatgaccttc
     1561 ttatcaaaac ccgaccaacg gcaattaatc tcaaatgggc cctcgacagg atgatagaca
     1621 cactgaagga tctctgtgta tcagagcgca aggatgtggc ctgggcgctg gcggcagaaa
     1681 ttgccgagga agatgtcgcg ttgtgtgaac aaattggatt acatggtgca gaagtcatcc
     1741 gcgaaatagc ccagaaaaaa ccggccggga gtgtggtcaa tatcctgaca cattgtaacg
     1801 caggctggct ggcgactgta gactggggaa ccgcgctttc tcccatctac aaagcgcatg
     1861 aaaacggaat ccctgttcac gtctgggtgg atgaaacgcg gccacgtaat cagggcggac
     1921 tcacggcatt tgaactgggc tcacacggta ttcctcacac cctgatcgcc gacaacgcgg
     1981 gtggccacct gatgcaacac ggtgacgttg atctctgcat tgttgggacc gatcgaacca
     2041 cggccagagg ggatgtctgt aataaaattg gtacctatct taaggcgctg gcggcccatg
     2101 ataaccatgt tcccttctac gtcgcgttac cttctcccac tattgactgg actatcgagg
     2161 acggaaaaag cattccgatt gagcaacgtg acggaaaaga gcagtcccat gtctatggga
     2221 taaaccctca gggagaattg agctgggtca ataccgcccc tgaaggaacc cgttgtggga
     2281 actacgcttt cgacgtcacg cctgcccgat acattaccgg cttcattact gagcggggag
     2341 tctgtgctgc cagtaagtca gcgctggcgg atatgtttgc tgacctgaag agtaaagcac
     2401 tccaggggga acagcattaa aacagcggcc attgctatta aggatcagga atggaacgga
     2461 ttaagttagc agaaaaaatc atttccactt gtcgggaaat gaatgcgtca ggtcttaatc
     2521 aaggaacatc aggtaatgtg agtgccaggt ataccggtgg catgttgatt acgccgagtg
     2581 ggatcgctta ttcaaagatg acgccggaca tgattgtctt tgtagacgat aagggaaaac
     2641 ctgaagcggg taaaatacca tcaagtgaat ggttaattca tctggcgtgt tataaagcca
     2701 gaccagaatt gaatgctgtc atacatacgc atgcggtgaa ttccacggcg gtggcaatac
     2761 ataatcattc aattccggcc attcattata tggtggcggt atcggggacg gatcatattc
     2821 cctgtattcc ctattatacc tttggcagtc ctgaactggc cgacggtgta tctaaaggaa
     2881 tcagagaaag taagtccttg ctgatgcagc accacggtat gctggccatg gacgtcacgc
     2941 tggaaaagac gttgtggctg gcgggtgaaa ccgagacgct ggctgattta tacatcaaat
     3001 gtggcggatt acatcacgat gttcccgtgc tgtctgaagc cgaaatgacc atcgtccttg
     3061 aaaaattcaa aacgtatggt ttgaaagcgt aattacagct ttattttaat taataacata
     3121 tgacagccat tcgcagaatg gctgctattc cctctgtcca caacgattga ttataactac
     3181 cacaaggagt tatttatgtt tatcgtggaa tcgtatgctg tcgccataat aatgtgtttt
     3241 attaccatga tttgctgggg ttcatgggcg aatacaacta aacttgtcag caataaaaaa
     3301 tgggagttcc ctctattcta ttgggattat tcaattggat tattgctgtg ttctttgtta
     3361 tttgccttca cgctgggttc tatgggcgaa gccggtcgca gttttatacc tgatattcag
     3421 caggcgagca gcagtagtct gatgtcggca atactggcag ggattatctt caatatttcc
     3481 aatattcttc tggtcgcttc tattaatctt gccggtatgg cggtagcttt tcctgtgggt
     3541 gtggggctgg cactggctct gggtgtgatc accacctata tcggcaatcc gcagggcgat
     3601 ccgctcattc tgttcctcgg tgtcgtctgc gttgtgatcg ccattatttt taccgcgata
     3661 gcctatggtc gtgtcacgca ggaggcggat aaatctcgcc gtaataaagg tctcattact
     3721 gccattctgg cgggtatcat catgggatgg tttttccgct tcctggcgga ctccatgtct
     3781 gacaatttca gccagcctgc cagcggtctg atgacgcctt actcagcact tgttctgttt
     3841 gccgtggggc tgtttttgtc gaactttgtc cttaacaggc tggtcatgaa aaaacctatt
     3901 tcaggagaac cggtcaacgg gaaaatgtat ttttccggga gtttacggga tcatgtttgc
     3961 ggctggctgg gcggtatgat ttggtgtgtc ggtctggcat tcagtctgat tgcgtctggt
     4021 caggcggggt atgccatttc ctacggtctg ggacaggggg ccacaatgat tgccgtgatc
     4081 tggggagtct tcatctggag agaatttgcc agtgcgccag ccggaaccaa taaactgctg
     4141 ctgaccatgt ttatttcata cattgtgggt attgttctta ttatcgccgc taatcaataa
     4201 aatcctgccc catatctaca tggggcagtt gttcattctt ttagtgtggt aattcacacg
     4261 ccagcaaaaa ctctgccgtt ccttcatcaa caatcaggtc cgtgacatat cctcccagca
     4321 gggcacccaa cgttgcgtca tagcctcttt ccccaccagc aaggaagatc ttccgttcaa
     4381 tctgccttag ctgagccaga ctgattccca gaatacgctg gtcaacatca gccacgacgg
     4441 gcatcccttc cttgtcatag aagcgaccac aaatgacacc tactgcgcct aaatcccgat
     4501 acgtctgcat ttccttctta ttcagcacgc ccacccgaat caggggattt tcatcaagcg
     4561 cgttacccac gacaaaaaag gctttcgtac agcgagtcag aacgtcaaaa ttcgatcgaa
     4621 taataggctc tgcctgtaac tccatggcca gtctggcgct tgataccacg gcgggcacgt
     4681 gcagcgaggc gacacggcct gaaagcttgt aggcgatttg cgatgaggat tcaatgatcg
     4741 tcaggtctgg ctgagatggc atggaaccga gcatctgtat aacggtgaca tttttacatg
     4801 acttcggcgt catggttttg ctcatctgat ggattgttct tccccaggca acccccagca
     4861 catcaccatt ctcaatcacc tgattcagat acatgccacc ggcacgtgaa aggcgagtcc
     4921 gcatcagctt tgtatcatca cgttttccgg catgttcgcc atccggcacg atgatcactc
     4981 tctggagatt gaatttatcg cgaatttgca aagcagcgtc gatagagcca aagacattca
     5041 catccaggtt gatatgaacc agtccatttt cccgggctgt ttgcaggtat ttgacaaccg
     5101 tgacgcggga cacccccatg atagctgcaa cttcagtctg gcttttacct tcctgatagt
     5161 acagccatgt tgcatgaagt acgggatcat cattgaacag gctgctgttt gtttgatctc
     5221 tatccacttt tgtatacctg tattaaaccg gggaaatgct catcttaacc ctgtttaccc
     5281 ggagagttga tgcagattgc cagtggcatg attttataga gatattttat tcttttacat
     5341 gaaataaacc agcataccat ttgagcgatc gcccgctgtt cccccatact gtaaagccgg
     5401 agacatgctc cggctttaca gtattactga gaatacaccc atcaagcata atcgaaggtt
     5461 caatatcgcc cctttttgat cgccagcgat tcgtcaccgt gcagacggtt gccgtcactc
     5521 actaaattat gggggacagt gaacaacagg ttggctcact ggcaagaacc cgattataaa
     5581 actaagctgg cgagcagaat aaaaatcagc ccactgatgg ataagagtgt ggacatcagc
     5641 gtccaggata acagcgtttc tttggtggtc aggccaaaaa agtctttaat catccagaaa
     5701 ctggcatcgt taacgtgaga acaaatacag gatccggcac cggtagccag cgtaatcaag
     5761 gccaggttgg tattgggatg tactgccaac aatgggatga ccagaccggc ggtagagatc
     5821 gccgcgacgg tggctgaacc cagacaaata cgcaggaatg cggccacgcc ccaggccatc
     5881 aggatcgggt taatatccat acccgaaacc agggtggaaa tatactgacc aacgccagag
     5941 tcgatgagaa cttgcttgaa tgcaccacca ccaccgataa tcagcagcag accggcaata
     6001 cccgcgatag cttttccgca agaatccatc aggtcaggga ttgttttccc gcgccccagg
     6061 cccatggtat agatcgcgaa tagcagtgaa atcagcatgg cgatcgttga attacctaag
     6121 aacagtatca cgttgtaaaa taagcctgaa tccgcggcac ttttggcatg agtcatctgg
     6181 atgatggtga caaccgccat caggatcacc ggcagcattg ccgtgagaaa actgatccca
     6241 aagcctggca tttcgctttc gctgaatcgg cgggtcgcgc cgagagaggc tatattcccc
     6301 tcttttttaa aggcgtccgg aataatgcgc tggcagaatt tattcaatac cgggccgcaa
     6361 agaataaagg ttggaatgcc gacaatgata ccgtatatca gtaccaatcc tacatcagcg
     6421 ccatattccc tggcgataac agtcgggccg gggtgcggcg gcaaaaaacc gtgtgcgacc
     6481 agcaaaccag aaagcatcgg cacacacata aacatcggtg atatttttgc ttcacgggca
     6541 atagcgaata aaataggtac cagaagaatt aaaccgactt cgaaaaaaag tgcgataccg
     6601 acaataaacg ccgaacagac cactgcccag tcaagtttat ttttcccgaa ataattcagc
     6661 atggtcagcg ctattcgctg cgcaccaccc gcatcggcca gcaagcgacc gagcataaca
     6721 ccgaagccaa atatcaaacc aatatggccc agcgtaccgc caagtccgga ttcgactgag
     6781 gtgacgactt tactcaaatc cataccactg gcgatggcga cagcaatcga gacaataatt
     6841 aacgaaacaa acgtattaag cttaattttt atggttaaaa gcaggagtaa agcaatccct
     6901 gccacaacga taattagtgg cataacgtcc tctcataaca gggtagcgac ccatcattat
     6961 ccgactgagc aaataacgaa tgatgcagag tcggataatt caggtgtctg gatgttttta
     7021 tttatgcttt cagtttttat tcataaaatc gcgcaaagcc gcgccaattt tgtttacatc
     7081 ataaatacaa ccggtccatg tcccaccact cacggcctga agcattgccc atagccgggt
     7141 atcatctggc aattcaggat cgggaagtaa atcctgatgg ctgggtctgg catttaatat
     7201 tgcagttgcc tcctcctgtg aaggtaattg ttcatcgcta cgggttccga ggaaattgac
     7261 ttcgccgtga agctcgcgac aatcaatttt aatttcaatt aaatccccgg tgcgtaattt
     7321 accgatgggg cctccggcca gcgcttctgg ccccacatgg ccgatgcacg cgccagtaga
     7381 aacgcccgag aaacgtgcat cggtgattaa cgaaacatgc ttaccgtatg acagatgctt
     7441 cagggcactg gtaacctggt aggtttcttc catccctgta cctgaaggtc caacgccaat
     7501 aatgaccaga atatcgcccg ccttgatctt gtcatgtttg atatcgtaaa tcgcactttt
     7561 ctcggacaga taaaccttcg ccacaccttt atggtaatag ataccttgct catcaatcat
     7621 cgaggggtca atggcggtgg atttgatcac cgaaccttct ggcgcaatat tgcccaccgg
     7681 gaaggtgata gttgaggtta atccgcgcgc ttttgcttgc tgcggagaca tgatcacttc
     7741 gtcagcgttg atttgttcct gatcgagcag gagttgcttg aaccgctgac gccgttcgga
     7801 gtgctcccac cagtcgaggt tttctttcag cgtgctgccg gtaaccgtca taacgtcttc
     7861 atgcaacaat ccgaggctgc gcagatgcaa catgacttcc ggcacaccac ctgccataaa
     7921 ggcattgacc gttggatgat aaaccgggcc attaggcagt acgctcacca gtcggggcac
     7981 gcgcttgttg atgcggatcc agtcatcaac ggtcgggata tggcaacctg cctggtgagc
     8041 aattgccggg atgtgtaaca gcaggtttgt tgaaccaccg aacgcggcat ggaccgtcat
     8101 cgcattctct atcgctttat cggtgagaat ttcccgggtg gtgatgcctt tttgactcag
     8161 gttcagcgca gctcgcgcgg aagctctggc gatctcccgc cacacaggct caccggaagg
     8221 ggccagggct gaatgtggga ttgccagtcc caatccttcg gccaccacct gagatgtccc
     8281 ggcagtgccc aaaaattgac agccgccgcc ggaagaggca caggctttac agcccgcacg
     8341 gcgtgcgtcc tgtagagata attcgccatt ggcgaagcgt gcgccaatgg tttgcacctt
     8401 gccgttgtct tctccatcct ttgcgggcag cgtcgcgccg ccggggacca gcacggttgc
     8461 gatgttgtgc tgcgcggcga gtgccatcat ggtggccgga agccccttat cgcaactcgc
     8521 cacaccaata actgctttcg cgtcgggcag agagcgaata aggcggcgca ttaccatcga
     8581 tgcgtcattt cggtatggta gcgaatcaaa catccccgtt gtaccctgag tacgcccgtc
     8641 acaaggatcg gagacgtaca cggcataggg cagggcatgg ttggctttaa taacctcggc
     8701 cgccgctttc atctggatgt ccagttcgta atgcccctgg tgcaacgcca gcgccacggg
     8761 tttaccgtct gcgccgcgta agccaccgag tgtactgagc agtaaaatac cgtcccgatc
     8821 cagctcgtcc ggagaccaac ccattccggc attcatggtc ataccgaaca gatccccgct
     8881 cgggcggttg ataagcatct ctgcggttaa tgggagttcg ccgtccgggc catcggcgtg
     8941 cgttctgacg gtgtaaatat cgtggctctc gtcagcaaaa atattgcgaa cagacatgag
     9001 tttctcctca gactggtaaa atgccctgcg cggtaagcag cacgtggact ttatcttttg
     9061 cttcttcaga tgcttcaaga atcggtggta agcaatatgt ctctacaggc agcccgacac
     9121 actgcatgct gtatttgatc agtgagacaa acggtgtttc gagggcgtaa atagcgggca
     9181 gttgtagtag ttttttattc agcgtcgcag cggtcgccag atcgccttca cgccaggcgc
     9241 ggtagatgcc gacggagagt tccggagcaa agttagcgct ggcggttatc gcaccgtcgc
     9301 cgcccagcag catcgtattc agcaaatgat catcgtaacc gcagaatacc gaaaacgacg
     9361 ggcgtaccga cttaactgtg ttgatcatcg tacgcaagtg accaacgctg tcgatggtgt
     9421 ctttgatgcc aacgatattc tcgttttgca gagccagacg cgtcacggtt tccggggtta
     9481 agtcctgacc cgtcagatcc ggaaagttgt acaggatcac cggtagggtg acgctacggg
     9541 cgatctgctg gtaatagtcg tcaagatttc gtggtgcgac tttccagtag taggggttga
     9601 tggcgacgat accatcagcg ccgtaggctt gcgcatgctg cgccagtttg accgcttcgt
     9661 cagtggaagg ggaaccgacg ccaatcaata ccggcactcg cccgtcgaca atggttacag
     9721 cttcttcggc gagtgccatg cgctgggctg tattcatttg gctaaattca ccaccggtac
     9781 ccagataaaa cagcccgtcg acccctttat taatcaggaa gtcggcaact tcgcgcattg
     9841 cctttttatc aagggttccg tcacgatgaa acgtgctgga taccggtgga ataatgccgc
     9901 tgaatttttt cattacctca gtttcctttt cgtaagtgct cattttcgtt tctgtatccc
     9961 agcccatatg atatttgctc agcgcatttc cggagcaatt ccagatagct gtctatctta
    10021 tttcctgaat aaactaccgg atcgccagaa agtgagattg catagttaac tctgttatac
    10081 atattgaata ccggcatact taaacatacc gccccatagg ttgattcttc gttgtctatg
    10141 gcccatcccc gaaggcgtgt tttttgtaat tcttccagaa acaatttttt atcggtaaat
    10201 gtattgcgcg tatgtggagt taacgtgagt gcttctaaaa aataatccag ttcttctcgt
    10261 gtcttccagg ctaataatgc cttacccaac gcggtaatat gcaactcaag ttttttgcca
    10321 atccagcttt tgctcgtcgg tacagagtcc ggactttcga tcttgtcgag atagatagcc
    10381 gaaatgctct ccatcgcccc aagatgacaa accagtccac ttttcagcga caactcctgc
    10441 attggccgct tagtcacctc aaatatattt tgacggtgta acgcctgaca acccaattcg
    10501 tagtttttta ttcccagtga atagtagccg tttttgtttt taattaagaa accacaatcc
    10561 accattacat tcaataaatt aagcaggctg cttttggggt actggaattt atttaataac
    10621 tccatataac ttgccatacc aacccatgca atgtgggtta atattttttc agcacggact
    10681 aatgaattac accctttgcg aaccataatg gctcctcctt gctcatggaa taaatggtat
    10741 cggcattaca tagtggaaaa tatgctttgt tcagcatgct gaaccgtaat gatgtgatta
    10801 aatgtgatct tgtacacact ttgaataatg aatccagaac agccacattt agtgaatgag
    10861 aataaaaagt agatggcgtg ttgttgcaac atcgtagtgt gtggattgcg cctagagctt
    10921 ctttattcgt tcgagcgcat cgccaatatt ttctattggg tgccattgat tatttttgct
    10981 tgaaatccct ctcgaatgaa cttacttatc actttattga atgaggtaaa aacaactgac
    11041 atccggctcg ctgataacgc tgtgcaatag tctcaggtaa cccgctgtca ctgatgatcg
    11101 ttttaataca tgatagtgtt gctacagcat gcggctcaac ggcatcaaat tttgagtgat
    11161 cggccatgag aataacttca cgcgagcgtt gaataatccg tgttttcacc ccgacctcaa
    11221 acatggtggc attggtgatc cccgtctcca gcgacagcgc atcgcaggag ataaacgcac
    11281 gttctacgga aaaggcattg atcatctcca gcgccagact ctctcctacc gaaaaataac
    11341 ctgggcgaat cagtcctccg ataatgtaac tttccacatg gggaaaacag cccagttcat
    11401 tggcgatttt aatgtcgttg cagatgacct ttacccgcgc atcggccagg catttcgcca
    11461 gttcgaggca ggttgagccg gagtcgagaa aaaagcagtc gccatctttg atcattgttc
    11521 gggcaagggc agctatttct cttttcgcca cagattgcaa tgtacgtttc acgtcgaaga
    11581 cgtactcttt gtcgtctgta ctgtcgtcga agtccaggca accgtgaccg cggatcatgc
    11641 ctggatactg gttgacaata tactggaaat cacgacggac agtcgcttcc gcatagccaa
    11701 aaagctccat ggcctgctgt gtggagaggt gacgatgttg ccacaggtag tgcagcatct
    11761 gtttgatacg gtcaggtctc tgttggctca tcagaaacaa tctcctctta cagggtggcg
    11821 aattgagcta aggtcgcgcg atagtcagat gaagagaaca gggcgcgccc aatcaccata
    11881 tgttgcgccc cagctgctgc cagttgctgt gcagctgcca gcgtaattcc accgtcggcc
    11941 cagcactcgg tctgaggaaa agccgtgcgt actttttgga ttttctcgca catggaggga
    12001 ataaaccgct gtccctgccc atcgggctca ctggtcatca ccatcactcc gtccagctca
    12061 gacgccagat atcgccaggc gtcgatcggg gttgccggat taaataccag tccggctcgt
    12121 gcgccggtat ggcggatctc agttaacgtt tccgaaggat agtccagcgt ttctgcatga
    12181 acgaagatcc acgcgggtct gatttcagcc agcgcgttaa accagcgctg tggccgggca
    12241 agcatgaaat gaaaggacaa gggatgaggc gtctgtctgg cgacggcctg caccgttttc
    12301 atcccaaacg taatgttgtt aatgaagctg ctgtcctcaa tatccagatg caggctccca
    12361 aaatccagat tgtccaatgc cgtcagctca cgaccatagt gcagaggatt tgcgctggcg
    12421 agagaagggt gcaggatcat aagcgcctcc gttaacctga taccagcgca aacagtgcat
    12481 cgtctgtgct ggcatttccc actgccgtta agcgttgtgg cgaatcaata aactggctga
    12541 tgcgctgaat ggtcaaaata tgtgcgttgg catccgtggc gctgacgcag agaagcagcc
    12601 agacggggtc gcactcctcg ttaccaaagg cgaccggggt gcgtagcgtg gtgatactga
    12661 cctgattata gtttgcgcct tgctctggtc gggcgtgagg caaggcgatt cctggggcta
    12721 tcagataata aggcccccag ttaagggtat tttcgatgat cccattaacg tagcatggct
    12781 gcgcggcacc ataggctacc agcgggcggg tggcgatttc cacggcctga cgccagtcgg
    12841 tggctttacg ttgtgcctgt acccatttaa tatcgttaat catccgtcgt tgactccatg
    12901 ccgattcggg ttaatctggt agcgatcccc gtcgatactt ttgacgaagg cggcagggat
    12961 cgcagaaggg ttattgctct ttgccgggga taagcgctct gcttatcccc atcttatcgg
    13021 cgtatatgat gcactttctc cataaactga ctgactcgcg cctggtcaac gaaattggca
    13081 aaaacgccgt cctttttaaa ggtggttgcg gtcacacagc catcggcgat actgagttgt
    13141 tcctccacgt tttccaggca gacgcccgtg ttggccaaca cgacggtgtc aggcaccgtt
    13201 tctttgaccc gttttaacag cgcgctgtcg gtacgcgtcc cggcggtcag gccagaaaca
    13261 cagagcgcat caggatggtt gttaaatact gtcgatttag caatagaaca gatatcgcga
    13321 ttgcccagat acacggcggc ctctgggacg atattgaaca aggttttcac ttcgcctgca
    13381 ccaatccggt gttgatgacg gatggtctca ccgacgttag tgtcccagac gccaaagtcg
    13441 ctggcatagg caccggtaaa tatctcgcgg ataaacttag cgccggtagc catcgccagg
    13501 tcgaatgagg cgaccggatc ccacagaaca ttcacgccaa acgggatgcg gatatcgctc
    13561 attaattgcc cgatgattcg tgccattgcc gcagtggttt ccggacgcac cttcgtgaga
    13621 tagggaagac taaactcgtt ggagaacatg actgcatcca ccccaccatt ttggagcgcc
    13681 atcagatcgt cccaggcttt gtcgataacc cagttcatcc ccaactgcgc atcaaaactc
    13741 gggtcgccgg gcaatgcacg tagatggcac atggcgatga ccgctttttc cgtcccaata
    13801 acctctttca gccaactcat tgaaagtact ccttattccg ataattcacg tttacgtaag
    13861 aacagcacga cgctggcgac aataccgacc gccacgacaa cgccaataat cccaagcgac
    13921 atcaattcag aaatcgacca accgaacata ttgcccacgg acaacgctga gatttgcgca
    13981 ctttcaccgg caaaactgaa accgcctttg agcgccattt cagtgaagta aggcgcgaac
    14041 tgggtggcga tcagcaggac ggtaatcatc acgatgaccc cgcttatcaa cgtcctgacc
    14101 aggtctccgc ggtggataac cgtagccata cagataaaga acggtgctac cggcaggtct
    14161 gcgaggggta acactttgtt gcccggtaaa atgctggcca gaattaacat aatgggtatc
    14221 agtagcagac cgacagcaat ggtggttgga tgccccagcg tcacagcagt atccagaccg
    14281 atatacactt cacgtccttt gaaatatttc tggaagaact ttcttgcacc gtcggagatg
    14341 ggcagcaggc cctcaacaat caggcggatc atgcgcggga acaacaccat gattgccgca
    14401 acggtaatca tcagactggc gcaaccttta aagccttcac cagccgccag gccaaaaatc
    14461 agaccgagca ctacgccgat aatcaccgga tcgccgacca tgccgtagcg tttctggatc
    14521 tcctgagcat cgatattgcg tcccttcatg aaagggattt tttcatagat agcatcaagt
    14581 agaacgaaaa gcggtactga actggaaccg tagccttgcg gtatcgaaat accttccagc
    14641 ccgacgatgt tctgtacccg ttttgccgtc cagtcggcca tttttaacga tagcgccgca
    14701 tggcagattg cccccagcac accataaatc aggctaccgg tcataagctg gaccaccgta
    14761 cccgtaatgg cgtagtgcca gtaattgtaa atatcgacgt tcatcgtttt ggtcagacgg
    14821 gtgaccagca tggcgacatt gagaagaaaa ataactggga taatcatggc gccaatggct
    14881 gtcgcataac caacgccgga ggcagggcct gcgccaacgt caaagacatg cagggctagg
    14941 ccgaaacgct caatcatcac tttgataggg ggactgaggc tgtcgatcgc catgacgatc
    15001 accagtccca taccgacaaa accaatccca accgttaccc ctgccttaat cgcctgcagc
    15061 caggggatgc ggaagataag gccaatcacg atcatgatga tagggacgaa aacggtaccg
    15121 ccgagagaca ggatgtaatc aaacataatg cctcctttta ttgagttaac agagccttga
    15181 tttgttgttt taacgcgtcg tcgttgatcc ctgtgagcag ggcggcgcca ttaagcgtag
    15241 ggatgccgta atcactgttg gttcgcatgg aggttacaat aaggtccata ccgttacagt
    15301 taagagggat ctcattcaga cagcattggg cggtagtggc tgaaatgccc tgttcagtca
    15361 ggaactcttg cagcttgtgt gcaatcatgg ttgaagtcga catgccggta ccgcatgcca
    15421 caaggatctt tttcataatg gatgtgcctc ttgtgtagtg cctgaatcag gctcaacggg
    15481 gaaatgtgct gctgacatac ctgccagagc ggtcaatagg cggatgcaat cagtcaaatc
    15541 acgcaggctg gcaacctccg ccggagaatg ggtatagcga caaggaatag agagactggc
    15601 gcagggaata ccatcctgct caacctggat ataaccggtt tctgttatca cgccgggagc
    15661 cacttctcgc tgcaccggaa tgttgtgttc aagagccgtc tgttccaaca tccgtatcag
    15721 gcgaggaggc gtgattaatc cggccagcgt tccccgacca tggtagttca ggcaggtgat
    15781 cccaacgccc tgattaatcc tgacctcgga ataatcgtgt aaatcagggg tgtcacacga
    15841 tggagtgata tcaataccaa tcgccaggtc aggttttaca cggcgtaata cgggaacaat
    15901 gccgcggata ttaaattctt cctgtaccga agccaccaga taaacggcga tatcaagctc
    15961 catagtactg atagcatccg ctacaccgag cagggcagta cagcctagac gatcgtctag
    16021 tgctttactg cacaccagat cgtttgccag gagttgcggc gggttataca gagtgacagg
    16081 cgtaccgacc tgaatgccca tccgtatagc gtcgtctttg tctttggcac caatatcaat
    16141 ccacagtttg tcgacagaag gtgactgcgt gcgctcgtcg cctttggcaa agtggtagga
    16201 cttaatgccg atacacccca tgactggccc tttgtccccg gtgagggtga cgatggaacc
    16261 agccatagtg acctgcgcag gaccgcctac gcgttcaaag cgtaaaaatc cagacggctc
    16321 tattttgcgc accataaaac cgacttcatc catatgcgca aaaatcatca gtcgcagcgc
    16381 atcgggttta tcactaccgt agcgcgcgac gacattccca agcctgtctc gccagacctc
    16441 ttttgcctga cgcctgaatt cgcacagcat gacgtcagcg acagcgtttt cgtgtcctga
    16501 aatcgcattg tgctgcaaaa gcgagaacag ggtttcctgc acagaaaatg acatagctat
    16561 ctccgtagac cgtaaaacaa tcactcgttg agcgttttat aggcgcagac tggtcgaata
    16621 attgtgagaa cgttcacgtg aaaacgcgca aagctgaaca aataaaaaat tatgtgagcc
    16681 gagtggaata aaagcgctca acgtttgtga aagttacggg ctggagagac cagtgattat
    16741 ggttgaaata atgaggatga ataaaacgcg gtaactgtta tagcaccact ttgttgcagg
    16801 acgctccctc ttcattacgt cctgcacgtg gataccccag catctccatc agcggatctt
    16861 agaatgtaat ggcgcgtggc aggtcgttgg ttcccagcat aatgtaagaa aacatcggat
    16921 ctcctgaata gattcagagg gagtatagat aatcagctca aatcactctc cgctttatga
    16981 gcccaggctg gcattgccgt cagataacgt tttgcacgct tatgtgcttc ctccgggccg
    17041 actttttgcc aggaatcaag gaagctggct aaacctgata ccgcttcttc ccagggtttg
    17101 agattgccgt tgctgtcgct gcattacgca caatattgat cactggcact gtgtgcatct
    17161 ggtgccgaca tcggcattcc gcaagcctga cagaactgtt catgttgtga catatgatgc
    17221 tccttatggt gacatcttga ttaataaaca tactgaatat gtattgtgca ggtgatacat
    17281 cagatcaaag caaaggtaaa gcctcgatat ttccagccag aaataccggg aacctgttgt
    17341 taccgggcga tgagtgcgaa cacgccccag ccaaagcatt cccgcgcgta ggtcacgtag
    17401 cgtttcggcg cgatgtttag ttcggccctg acttcggcgg cgaagtcatc gtcaggattc
    17461 gcttccagcc agcggcgcat ggtcagccat tttgcggctt cataccggtc ccagccttcc
    17521 tggtctgcca gtaccatttc caccacgt
//
LOCUS       DAYPHN010000102        17244 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000102 DAYPHN010000000
VERSION     DAYPHN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.102"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            546..827
                     /gene="drpB"
                     /locus_tag="ACG0BW_003162"
     CDS             546..827
                     /gene="drpB"
                     /locus_tag="ACG0BW_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFK7260089.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            867..1562
                     /gene="yedJ"
                     /locus_tag="ACG0BW_003163"
     CDS             867..1562
                     /gene="yedJ"
                     /locus_tag="ACG0BW_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFK7260090.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1629..3047
                     /gene="dcm"
                     /locus_tag="ACG0BW_003164"
     CDS             1629..3047
                     /gene="dcm"
                     /locus_tag="ACG0BW_003164"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFK7260091.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTSVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3028..3498
                     /gene="vsr"
                     /locus_tag="ACG0BW_003165"
     CDS             3028..3498
                     /gene="vsr"
                     /locus_tag="ACG0BW_003165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFK7260092.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGLGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3487..4407)
                     /gene="yedA"
                     /locus_tag="ACG0BW_003166"
     CDS             complement(3487..4407)
                     /gene="yedA"
                     /locus_tag="ACG0BW_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFK7260093.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVLLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4580..5497
                     /gene="yedI"
                     /locus_tag="ACG0BW_003167"
     CDS             4580..5497
                     /gene="yedI"
                     /locus_tag="ACG0BW_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFK7260094.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5576..5758
                     /gene="yodC"
                     /locus_tag="ACG0BW_003168"
     CDS             5576..5758
                     /gene="yodC"
                     /locus_tag="ACG0BW_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFK7260095.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            5929..7623
                     /gene="dgcQ"
                     /locus_tag="ACG0BW_003169"
     CDS             5929..7623
                     /gene="dgcQ"
                     /locus_tag="ACG0BW_003169"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFK7260096.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSDNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7620..8435)
                     /gene="yedP"
                     /locus_tag="ACG0BW_003170"
     CDS             complement(7620..8435)
                     /gene="yedP"
                     /locus_tag="ACG0BW_003170"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFK7260097.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTTRLHELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(8733..8960)
                     /gene="yodD"
                     /locus_tag="ACG0BW_003171"
     CDS             complement(8733..8960)
                     /gene="yodD"
                     /locus_tag="ACG0BW_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFK7260098.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SADGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9123..9311
                     /gene="dsrB"
                     /locus_tag="ACG0BW_003172"
     CDS             9123..9311
                     /gene="dsrB"
                     /locus_tag="ACG0BW_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFK7260099.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9355..9978)
                     /gene="rcsA"
                     /locus_tag="ACG0BW_003173"
     CDS             complement(9355..9978)
                     /gene="rcsA"
                     /locus_tag="ACG0BW_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFK7260100.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10268..11053)
                     /gene="fliR"
                     /locus_tag="ACG0BW_003174"
     CDS             complement(10268..11053)
                     /gene="fliR"
                     /locus_tag="ACG0BW_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFK7260101.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11062..11331)
                     /gene="fliQ"
                     /locus_tag="ACG0BW_003175"
     CDS             complement(11062..11331)
                     /gene="fliQ"
                     /locus_tag="ACG0BW_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFK7260102.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11341..12078)
                     /gene="fliP"
                     /locus_tag="ACG0BW_003176"
     CDS             complement(11341..12078)
                     /gene="fliP"
                     /locus_tag="ACG0BW_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFK7260103.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12078..12443)
                     /gene="fliO"
                     /locus_tag="ACG0BW_003177"
     CDS             complement(12078..12443)
                     /gene="fliO"
                     /locus_tag="ACG0BW_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFK7260104.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12446..12859)
                     /gene="fliN"
                     /locus_tag="ACG0BW_003178"
     CDS             complement(12446..12859)
                     /gene="fliN"
                     /locus_tag="ACG0BW_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFK7260105.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12856..13860)
                     /gene="fliM"
                     /locus_tag="ACG0BW_003179"
     CDS             complement(12856..13860)
                     /gene="fliM"
                     /locus_tag="ACG0BW_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFK7260106.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13865..14329)
                     /gene="fliL"
                     /locus_tag="ACG0BW_003180"
     CDS             complement(13865..14329)
                     /gene="fliL"
                     /locus_tag="ACG0BW_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFK7260107.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14434..15561)
                     /gene="fliK"
                     /locus_tag="ACG0BW_003181"
     CDS             complement(14434..15561)
                     /gene="fliK"
                     /locus_tag="ACG0BW_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFK7260108.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGEATTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAHQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAAADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEIISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15558..16001)
                     /gene="fliJ"
                     /locus_tag="ACG0BW_003182"
     CDS             complement(15558..16001)
                     /gene="fliJ"
                     /locus_tag="ACG0BW_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFK7260109.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQHAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16020..>17244)
                     /gene="fliI"
                     /locus_tag="ACG0BW_003183"
     CDS             complement(16020..>17244)
                     /gene="fliI"
                     /locus_tag="ACG0BW_003183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFK7260110.1"
                     /translation="TCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARV
                     YAKNISTEGLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQR
                     TPIEHVLDTGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIG
                     ERGREVKDFIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVL
                     LIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFY
                     TVLTEGDDQQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQH
                     YARVRTFKQLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADW
                     EASLQGLERIFPTVS"
BASE COUNT         4237 a         4310 c         4562 g         4135 t
ORIGIN      
        1 agattacatg taacaaatgt atttaaaaga tttcaaaatg tttctaatct atttaatata
       61 attcagaatt atgaattcta aatataaata ttcatgaaaa ttataaatat atatcaatat
      121 gttatgagtt gagtggaaac atagggggta tcaatgattg ttgcgccaaa cggctggtgt
      181 gatcacgcgc acattaatga aggcataaaa aaagccggca tcatgccggc ccaaaaaaaa
      241 ataaacaaga cgataaaagg ctaataacgg aggcatcatg acacagaaaa ataaaaaggt
      301 ataattgtaa tcagtgctaa tatattgatt gaatgaatta ataaagccga tatcaaagat
      361 aaataaaatc gctcaaaacg atttttcctg tattcactgc cattgcgcaa aagttatcta
      421 tttgttcaaa aaatgattga gtcttgactg gctcatccaa tgtggaaaaa tgtgactttt
      481 atcacataat agtactaagt ctgaattttc cgggttatct caaaatggaa tacggttcga
      541 caaagatgga agagagactc tctcgcagcc ctggcggcaa attggctttg tgggctttct
      601 atacatggtg tggctatttc gtctgggcaa tggcgcgtta catctgggtg atgagccgga
      661 tccctgatgc cccggtgtcg ggatttgaaa gcgatttagg ctctaccgcg ggaaagtggt
      721 taggggcact ggtcggattt ttatttatgg ctctggtcgg ggcattgtta ggcagcattg
      781 cctggtacac ccgacctcgt cccgcacgta gcagacgcta tgaataaaaa ctgctaaggt
      841 tgcgactctt tggcaacgga gcgcgtatgg acttacaaca ctggcaggca cagttcgaaa
      901 actggttgaa aaaccatcat cagcaccagg acgcggccca tgacgtgtgt cattttcgcc
      961 gcgtctgggc tacggcacag aagctggcgg cagatgacga tgtcgatatg ctggtgattt
     1021 taaccgcttg ttattttcac gatattgtga gcctggcgaa aaatcatcca caacggcagc
     1081 gttcttcaat cctggcggca gaagagacgc gtcgcctgct ccgtgaagag ttcgtgcaat
     1141 ttccggcgga gaaaatcgag gccgtttgtc atgccattgc cgctcacagt ttcagcgcgc
     1201 aaatcgcccc cttaacaacg gaagctaaaa tcgtccagga tgcggatcgg ctggaagccc
     1261 tgggggcgat tggcctggcg cgcgtgtttg ccgtttcagg ggcattgggc gtggcgctgt
     1321 ttgatggcga agatccgttc gcacagcatc gcccgctgga tgataaacgc tacgcgctgg
     1381 atcatttcca gactaagttg ctgaagctac cgcaaaccat gcaaaccgca agaggcaagc
     1441 agctggcgca gcacaacgcg caatttttag tcgagtttat ggcgaagctc agtgccgaac
     1501 tggcggggga gaatgaaggt gtcgatcaca aggtgataga tgcgttttca cccgcgggtt
     1561 gagcgcgtgg ccctaaatgg ctgtaattat gttaacctgt cggccatctc agatggccgg
     1621 ttaaatctat gcaggaaaat atatcagtaa ccgattcata cagcaccggg aatgccgcac
     1681 aggcaatgct ggagaaactg ctgcaaattt atgatgttaa aacgctggtg gcgcagctta
     1741 atggtgtggg tgagaatcac tggagcgcgg caattttaaa acgtgcgctg gcgaatgact
     1801 cggcatggca ccgtttaagt gagaaagagt tcgcccatct gcaaacattg ctacccaaac
     1861 caccggcaca tcatccgcat tatgcgtttc gctttatcga tctatttgcc ggaattggcg
     1921 gcatccgtcg cggttttgaa tcgattggcg gacagtgcgt gtttaccagc gaatggaaca
     1981 aacatgcggt acgcacttat aaagccaatc attattgcga cccggcgacg catcatttta
     2041 atgaagatat ccgcgacatc accctcagcc ataaagaagg cgtgagtgat gaggcggcgg
     2101 cggaacatat tcgtcaacac attcctgaac acgatgtatt actggcgggt tttccttgtc
     2161 agccattttc gctggctggc gtatcgaaaa agaactcgct cgggcgggcg cacggttttg
     2221 cctgcgatac ccagggcacg ctgttctttg atgtggtgcg cattatcgac gcgcgtcgtc
     2281 cggcgatgtt tgtgctcgaa aacgtcaaaa acctgaaaag tcacgaccag ggtaaaacgt
     2341 tccgcatcat catgcagacg ctggacgaac tgggctatga cgtggctgat gcagaagata
     2401 acgggccgga cgatccgaaa atcatcgacg gcaaacattt tctgccgcag caccgtgaac
     2461 gcatcgtgct ggtgggtttt cgtcgcgatc tgaatctgaa agccgatttt actctgcgtg
     2521 atatcagcaa atgtttccct gcacagcgag tgacgctggc gcagctgctg gacccgatgg
     2581 tcgaggcgaa atatatcctg acgtcggtgc tgtggaagta cctctatcgt tatgcgaaaa
     2641 aacatcaggc gcgcggtaac ggcttcggtt atggaatggt ttatccgaac aatccgcaaa
     2701 gcgtcacgcg tacgctgtct gcgcgttatt acaaagatgg cgcggaaatt ttaatcgatc
     2761 gcggctggga tatggcaacg ggtgagaaag actttgacga tccgctgaat cagcaacatc
     2821 gtccacgtcg gttaacgcct cgtgaatgtg cgcgcttaat gggttttgaa gcgccgggag
     2881 aagcgaaatt ccgcattccg gtttcggaca ctcaggccta tcgccagttc ggtaactcgg
     2941 tggtcgtacc ggtctttgcc gcggtggcaa aactgcttga gccaaaaatc aaacaggcgg
     3001 tggcgttgcg tcagcaagag gcacaacatg gccgacgttc acgataaggc cactcgcagc
     3061 aaaaatatgc gcgcgattgc cacgcgtgat acggcgattg agaagcgcct cgccagtctg
     3121 ttaaccgggc tgggcctggc gtttcgcgtt caggacgcca gtctgcccgg acgcccggat
     3181 tttgtcgttg atgaatatcg ctgcgtgata tttacccacg gctgcttctg gcaccatcat
     3241 cactgctatt tgtttaaagt gcctgcgact cgaaccgagt tctggctgga gaagataggt
     3301 aaaaatgttg aacgcgatcg ccgcgatatc agtcgcttgc aggaactcgg ctggcgcgta
     3361 ttgattgtct gggagtgtgc gttacgtggg cgcgagaagc tgacggatga agcgcttagc
     3421 gagcgtctgg aagagtggat ctgcggcgaa ggtgccagcg cgcagatcga cacgcagggg
     3481 attcatttac tcgcttgatg cgtcctgaat aacgggcgca actacgggtt ttgccgggaa
     3541 gagatatttt cccaacgtga ccagtaacac cgcgaagaca atcacgccga gcgccagcca
     3601 ttcaatcttc gacagcgtct ctccgcccag tcccgtaccc agcaagaccg cgaccaccgg
     3661 gttaacgtag gcgtagctgg tggcgagagc cggactgacg ttgcggatta aatacatata
     3721 agcgttaatg gcgataatcg aaccaaacag cgccagatag ccgaccgcaa ggaagcctga
     3781 aagggaaggg agcgccgtca gtttttcacc cgcaatcatc gacgcgatca ttaacaccac
     3841 gcctgccgcc agcatctcaa tcgcacccgc catcatccct acaggtaagg taatgcgcga
     3901 gccataaacc gagccaaacg cccagctaat cgagccgatt aaaatcagaa tcgcgcccca
     3961 cggattgccg cttaaatttc caccgctatt gagcatgatg attccggcaa gcccaatggc
     4021 gatacccacc cattccagtt tgcgcgtttt aatgccaaac aggcggctga agcacagggt
     4081 aaagaggggc acggttgcaa ccactacggc ggcgatgccg gaaggaacat tttgatgctc
     4141 ggcaaccgtc accatgccat taccgacagc caataataac aggccaatca gtgcggcatt
     4201 gagcagcgga cgtagcgggg gaagtttgtg tccgcgcagt agcaaaaatg ccagcaataa
     4261 aataccggct gccaggaatc gcacgcccgc catcattaac ggcggccagc tttccacgcc
     4321 aatccgaatg acaaaatagg ttgagcccca aatgatatac aacgcaaaca acgcgccaaa
     4381 aagcggtaac aactggcgga aacgcataat ccctcacggc ggaaataaaa aggtggttca
     4441 tagtaaacgt gaaaatcatt ctgctggcga gagatataat tgcacttgat tgttaaaaaa
     4501 atgttgacct gtgaagcaag tatcagagct accgtttttg cttcatactt acacccttca
     4561 acaataaaaa tgagagggaa tgcttttggc cgggagtagt ttactgacgt tgctcgatga
     4621 tatcgccaca ctgctggacg atatctccgt gatgggcaaa ctggcggcga agaaaaccgc
     4681 cggtgtatta ggggatgact tatcgctcaa tgcgcaacaa gtttcaggcg tgcgggccaa
     4741 ccgggaactt cccgtggtct ggggcgtggc gaaaggatcg ctgatcaata aagtgattct
     4801 ggtgccgctg gcgctgatca tcagtgcgtt tatcccgtgg gcgattacgc cactgttgat
     4861 gattggcggc gcgtttctct gctttgaagg ggtagagaaa gtgctgcata tgctggaggc
     4921 gcgtaaacat aaagaagatc cggcgcagag tcagcagcgt ctggagaagc tggcggcgca
     4981 ggatccgctg aagtttgaaa aggacaaaat aaaaggggcg attcgtaccg attttatatt
     5041 gtctgcggaa atcgtcgcca tcacgctggg gattgtggca gaagcgccgt tgcttaatca
     5101 ggtgctggtg ctttcaggca tcgcgctggt agtgaccgtg ggcgtctacg gtctggtagg
     5161 ggttatcgtt aagattgatg acctggggta ttggctggcg gaaaaatcca gcgcgctgat
     5221 gcaggcatta ggtaaagggt tattgatcat tgcaccctgg ttgatgaaag cattatcgat
     5281 tgtcggcacg ctggcgatgt tcctcgtcgg cggcgggatt gtggtacatg gtattgcgcc
     5341 gctgcatcac gccattgaac atttcgccgg gcagcaaagt gcagtggtgg cgatgatatt
     5401 accgactgtt ttaaatctga ttcttggatt tatcatcggc ggcatcgtgg tgctgggagt
     5461 gaaagcagta gcgaaaatgc gcggtcaggc acattaatac tcgcactggc tattatgcaa
     5521 aattcgccat cttcaactaa ggtgaacaag tttcactcga aaaaaggagg cgagtatgag
     5581 ctttatggtt agtgaggaag ttacggttaa agagggcggc ccgcggatga ttgtcactgg
     5641 atactccagc ggtatggttg agtgtcgttg gtatgacggt tacggggtca agcgggaagc
     5701 ttttcatgaa accgagcttg ttccagggga ggggagtcgt cctgcggaag aagtttgaca
     5761 gggtatcatt aagaccaggc cggatcacat ccggcctggt gtgccaatgc ctgcaatgaa
     5821 tgatagtcgc gtcttatcat gccaccatta taaaattatc gctgttttgc ggtacgttat
     5881 cgccgggtta tgccagaatc ataaaaaagc aggttgggag tcgtcagggt gcagcacgag
     5941 acaaaaatgg aaaaccagag ctggttgaaa aaactcgcac gccgcctggg gcctggtcat
     6001 gtcgttaatc tctgctttat cgtggtattg cttttttcca ccttgctcac ctggcgtgaa
     6061 gtggtggtgc tggaagatgc ctatatctcc agccagcgta atcatctgga aaacgtagcc
     6121 aacgcgctcg ataagcattt gcagtataac gtcgacaaac tgatcttttt gcgtaatggc
     6181 atgcgcgaag ctctcgtagc gccactggat ttcacctctc tgcgtaatgc cgtaaccgag
     6241 ttcgaacagc atcgtgacga gcacgcctgg caaatcgaac tcaaccgacg acgcaccctg
     6301 ccagtcaatg gcgtgtcgga tgcattagtc agcgagggga atctcctgtc tcgcgaaaat
     6361 gaaagcctcg acaatgaaat taccgctgca ctggaagttg gttacttgct gcgactggcg
     6421 cacaactcct cgtcgatggt tgaacaggcg atgtatgtct cgcgtgccgg attttacgtt
     6481 tcgacgcagc cgaccttgtt tacgcgcaat gtaccaacgc gttattacgg ctatgtcacc
     6541 caaccctggt ttatcggcca ttcgcaacga gaaaatcgtc accgcgcggt acgctggttc
     6601 acatcgcaac cggaacacgc cagcaatact gaaccgcagg ttaccgtcag tgttccggta
     6661 gacagtgata actactggta tggcgtgctg gggatgagta ttcccgtgcg taccatgcag
     6721 caatttttaa gaaatgccat cgataaaaac ctcgatggtg agtatcagct ctatgacagt
     6781 aagctgagat tattgacctc ttccaatcct gaccatccaa cagggaatat ttttgatcct
     6841 cgtgaactgg ccttgctggc gcaggcaatg gaacatgaca cacggggcgg cattcgtatg
     6901 aacagtcgtt atgttagctg ggaacgtctg gaccatttcg acggtgtgct ggtgcgcgtc
     6961 catacgctaa gcgaaggcgt gcgtggtgat ttcggcagta tcagcattgc attaaccctg
     7021 ctgtgggcgc tctttaccac catgttactc atctcctggt atgtgattcg ccggatggtc
     7081 agcaacatgt atgttttgca aagctcgttg cagtggcagg cgtggcacga caccttaacg
     7141 cgtttatata accgtggcgc actgttcgaa aaagcccgtc cgctcgctaa attgtgtcag
     7201 acgcaccaac atcctttttc tgtcattcag gtcgaccttg accattttaa agcgattaat
     7261 gaccgctttg gtcatcaggc gggcgaccgt gttctttctc atgctgccgg attaattagc
     7321 agttccttgc gtgcgcagga cgttgccggg cgggtcggtg gtgaggagtt ttgtgtgatt
     7381 ctgccaggcg cgagtctgac gcaggctgcg gaggttgcag aacgtattcg cctgaagcta
     7441 aatgaaaaag agatgttgat cgccaagagt acgacgatac gcatcagtgc ctcgctgggg
     7501 gtaagcagca gcgaggaaac cggtgattat gattttgaac aactccagtc actggccgac
     7561 cgtcgacttt atctcgctaa acaggccggg cgcaatcggg tattcgcgag cgataacgct
     7621 taatgggcgg agaaaaaatg gtccagccct tcacgccagc cttccggtcc ttcacgctgc
     7681 gttcgccaga cgcgggctgg atcctcatca tgcagatgca ccccttcacg gtttaaccct
     7741 ttcacaatca ccgcgtaatc cattacctcc agtaagggtg catcgtttgg cccatcgccc
     7801 aggccaagtg tggttgggcg tttgcctgac aattgttgat aggtcgcgat aatccagttg
     7861 gcagcctgat cttttccggc agaggcatcc aggacgtgcc agaagcgcgc accctgcata
     7921 aactgcaagc ccagctcgtg cagacgagtg gtaaattgtg ccatacgctc gtcactgtcg
     7981 cgccagatta gcgttaccga cgcctcatgc agctgcgtca gcgccgcctg gctacggctt
     8041 aatcctgtcc attcggcgat ggttgcatcg tcgacatcat caaaagttgt gaatttaaaa
     8101 tgttctttct cgcgtagcgt atttaaaacc tggctgattt cgccatggct aatacctgag
     8161 atgatgcgtg gaaaaccgtc tatatcctgc cattgctcag caagctggat cactgcgcca
     8221 ttttcggcaa tcagcggtaa accttgtagc cccaacattt tttgcaagta cagcatttcc
     8281 gctgatgtct tactgctaca gagaatgacg ggaacattcg cttcgcgtaa acggctgagc
     8341 cagggggcag ctggttgcca gtcataacta tgactgtcca gcagggtgcc atcaagatcg
     8401 ctaaaaacca gtagtggttg ttgaattgaa agcatggttg cgcagtactc cccttaccag
     8461 gatgattctt aaaacagcac aaattatcaa agatgatttt ttcgggagcg atcccgttga
     8521 cgccagagcc accaaggggt gacgcgcgtc acatttctat tcataagtag cgttaatcat
     8581 tcatatcacg aatattttct tgtcagcgaa aaaaattgcg gataaggtga tgaacacatc
     8641 agatttcctg gtgtaacgaa ttttttaagt gcttcttgct taagcaagtt tcatcccgac
     8701 cccctcaggg tcgggatttt tttattttgc attcaacgat tcacttcaga gacgctgaag
     8761 tcatgaatat ccagttcgaa ggcatccgcc aattcacgcc aagtatgata ttcacgtcca
     8821 tcggcgctaa cgtgttcaga atcgttctgg ctgcgatgaa cttcgctttc gctaatttca
     8881 ttgatcgccg ccagcagggc atcgacatcg acgctgacct cacgttttgc ggtatcgctg
     8941 tactcttttg cggttttcat catctttcct cgcaaccgtt gctctgttgt tgccgccagt
     9001 cgttaagtcc gcctggcatc tcttaagtat agaccgtcga gaaaatcagc actcgcgcgc
     9061 gcctggcgct gccagaaaaa cgaaattgtt ctacactggc acaaagccac aggaggaaaa
     9121 cgatgaaggt gaatgatcgg gtaacagtca aaacggatgg cggtccgcgt cgtcctggcg
     9181 tggtactggc agttgaggag tttagtgaag gcacaatgta cctggtttcg ctggaagact
     9241 acccgctcgg catctggttc tttaatgaag cagggcatca ggacggtatc tttgtggaga
     9301 aagcagagta atctaattca gcctgactgg tgggaaacca ccagtcagaa tgtgttagcg
     9361 catgttgaca aaaataccat tagtcacatt atccgtcagt cggacgacat ggtagataac
     9421 ctgtttatta tgcgttttga tcttacgttt aatattacct ttatgcgatg aaacggtctt
     9481 ggctttgata ttcatttggt cggagatttg aatggttccc tgacctgcca tccacattcg
     9541 caacatactc gattcggttc ggctcaatga taacgtcggc atatttaaaa acgaggttat
     9601 cgttgtctct tttttcagaa tatcgcccag gatatcgtcg agagattccg gtttaatcga
     9661 tttagaactg atcaataaat tttttctgac caatagatat tcatcaaaat gaacattggc
     9721 aattgccata aaaacgataa ataacgtatt gggatgttga ttaatgatga gcttgatacg
     9781 ctgactgtta gaagcatcgt ggatgaaaca gtcctcatta ataaacacca ctgaagggcg
     9841 ctgtgaatca caagctatgg caaggtcatc aacggtttca atgtcgttga tttctctttt
     9901 tttaacccct ctactcaaca gatacccggt taaacctagt cgggtgtaac tacataaatc
     9961 cataataatc gttgacatgg cataccctca ctcaatgcgt aacgataatt ccccttacct
    10021 gaatatttca tcatgactaa acggaacaac atgggtcacc taatgcgcca ctctcgcgat
    10081 ttttcaggcg gacttactat cccgtaaagt gttgtataat ttgcctggaa ttgtcttaaa
    10141 gtaaagtaaa tgttgcgata tgtgagtgag cttaaaacaa atatttcgct gcaggagtat
    10201 cctggaagat gttcgtaggt attatttttc ggataatcct taggataaca tgataaacgt
    10261 tacggaatta tattaatggc aattcactaa taatatcagc cagcaaatta aacatctcac
    10321 tgaataaatg ttcgcaaaaa ggcgcaatta acggcattaa tgccgccatt aaagagatac
    10381 cgacagtcag agttaatgga aatccaataa caaaaatgga taattgcggg gccatacgat
    10441 taagtaagcc taatgccaga ttcagggtca gcagcagagt aatgagcggt aatgccagca
    10501 tcagcccgtt aaggaaaatc aaactccctg ctttggtgag tgccagaaac gcattgctgt
    10561 tcagtggttc gccaccaatt ggcagggtgt gaaaggtatc gaccagcagt gaaatcaacc
    10621 ataaatgacc gttaaatgtc aggaacagca ataatgccag catatccatg atacgcgcta
    10681 gaacgggcat attgagatgg ctggccggat cgacaaacgt cgcaaatgac agccccattt
    10741 gcagaccgat aatttcgcca gcggttcgta cagcggcaaa ggcaaattgc atggtaaaac
    10801 caagcgcaat gccgatcagg atctgctgca cggccagcca cagagcaaag aacgaaaaaa
    10861 caggaacatc gttggcaggt aatgatgggg caatagcgaa cgtgatcatc attgccagac
    10921 ccagttttac ccgtttcggc acgctgcgtt cgctcagaat tggtgcggta gaaatgagcg
    10981 ccagcacgcg cagtaacggc cagaagtaca ggcttaacca ggaaagccat tgatcgcttg
    11041 tcacctgcat cattgtacgg cctacccgat gatatacggc aggttagtga acaaggtgcg
    11101 gacgtaatcc agcaacagat tgagcatcca cggtccggca ataataatgg cgataaatac
    11161 ggcgatgatt ttcggaataa acgacagcgt catttcgtta atctgcgtgg cggcctgcaa
    11221 aatactgatg ataaggcccg tgaccagcgc caccagcaac agcggggcag ccagtgccag
    11281 cgcgactttc atcgcttcag tccccatcat catgaccgat tcaggtgtca ttttgcctct
    11341 ctagctgtaa aagctctgcg ccagcgaacc gaccagcaat tgccagccat ccaccagtac
    11401 aaacagcatc agtttaaagg gcagagcaat ggtggctggg ggaaccatca tcatccccaa
    11461 tgccatcaac acgctggcaa tcaccaggtc gataatcaaa aaaggaatga aaatcgtgaa
    11521 gcctatctga aatgcggttt tcaactcgct ggtcacgtaa gccgggagca aaatgcgcat
    11581 cggcacggct tcaggtccct gcaacgggcc ggtattcgcc agtctggcaa acaaccctaa
    11641 atctgcctca cgggtctgac gcagcataaa ctcacgcagc ggctgcgccc ctttttccag
    11701 cgcctcctgc attgatattt tctcttcgct aaatggctgg tacgcatcga cataaatttt
    11761 gtcgatcacc ggcgacataa taaaaaaggt caaaaacagt gccagcccca gcaatacctg
    11821 gttaggtggc gcggagggcg ttccaagcgc gttacgcaat aaaccaaaaa caatgatgat
    11881 gcgggtgaaa ctggtcatca tgagtaaaat tgccggaata aacgtcaacg aggtgatgaa
    11941 taccagcgtc tgcaccggga gcgaccagct ttgtccaccg ccaggcagcg gctggctggt
    12001 gatacccggc agttgcgcga aggcgagggg cgtaaccagc cagagaagga caggtgcgac
    12061 agacaacaaa cgacgcatca ggatctcccg ctacgtttaa gcaagttttt catgaccgac
    12121 tgaaaatcgg tctgcggtat ctcttccgtt ggtgctgaag ggggaagttt atgcagcaga
    12181 ttgatttgcc cagcggtaac gccgagcacc agccgtgcat cttccacatc gactaccacg
    12241 acccgttcac gcgcgcccag cgaagcactg gcgctaattt tcagaccgtt aacgccagtg
    12301 cgtttcgggg caaatcccag ccgttttacc agccaggcag cagcgaggat cagggcaata
    12361 atggcgatga gtgcgccgct cacctgcagc agtggcgcag cagaaaccgg cgcggaagat
    12421 tgcacagtag cgtggttatt catcactaac ggctcaggcg gcgcatccgc tcagacggag
    12481 taatgatatc ggtgatccgc acgccatatt tatcagcgac tacgacaact tcgccctgag
    12541 caattaaata accgttgatc agaatatcca gcggttcgcc cgccagaccg tccagcgcca
    12601 cgactgatcc ttgcgtcaga cgcaataact ctttgatggt cattcgtgtg cggcccagct
    12661 cgacggtcag cttgaccgga atatccataa tcaggtcgat atcctgcaac gttccgctga
    12721 catcaccacc gccaaattgc tggaacaccg cgtcggcagc gcttttgccg ctggttgatt
    12781 tttgttcgct caacgcttca gcccacagat cgtccattgc gccgttgttg tcatcggccg
    12841 gattattcat gtcactcatt tgggctgttc ctcgttcaga gaatttaaaa tcgggttaat
    12901 caaatgttct atccgtaacg catactggcc gttgagagtg ccgtactgac tggtcagcac
    12961 cgggacgccg tcaacatggg cgatgatgcg atcgggtttt tctatcggca ggacgtcgcc
    13021 gggtttcagt tttaaaatct gcgacaggcg tagcgagata tcggcaaagt tggcgaccag
    13081 ctccagctgc gaatgctgca cctggcgcac caggttatcg cgccagttct gatcttcatt
    13141 acgcgagttt tccagcggcg ggttaaccaa caattcccgt agcggctcga tcatgctgaa
    13201 tggcaggcag atattaaatt cgcctgtcag gttgccaatc tccacatgga acggcgtgtt
    13261 aaccacgatg tcgttcggcg aggtggtgat attggtaaat ttcacctgca tttccgaacg
    13321 cacgtactca acttccagcg gattaatcgc cttccaggcg tcgctatagc cttcaagcgc
    13381 cagctttaac atgcggttga tgacacgctg ttcggtatgg gtaaactcgc gaccttccac
    13441 tttggtcggg aagcgtccat cgccgccaaa caggttatcc acggcgataa acaccagact
    13501 cggtgagaac accaccagcc ctgtgccgcg cagcggtttc agatggataa ggttcaggtt
    13561 ggtcggcacc ggcaggttgc gggcaaattc atggtacggc tgaatgcgga tggccccgac
    13621 ggtaatgtcc gggctacggc gcagcaggtt gaacagcccc atacgaaaat ggcgggcaaa
    13681 gcgctcatta atgatttcca gcgcctgcaa acgttcgcgc acaacccgtc gttgggtatt
    13741 cggatcgtac ggacgaatgt cactttcgcc gctaacactg gctgtcggtt cgtctttgac
    13801 ttcgctgtca ccattcaaca gcgcatcaat ttcagcttga gaaagaatac tatcgcccat
    13861 gtcgttatcg cagaataaaa gcggtataca gcacgtcggt gacatcctgt ttcggttgcc
    13921 cggcaacaag cggggtggaa agtgtggttt taatctcggc aatcaggttt ttcttgcctt
    13981 cttctgtcgc cagtacggca gcatcctgac gcgaaaacag taacagcaag cgactacgga
    14041 cttccggcaa atactcactc agccgcgagc gggtagcttc atctttcagg cgcagggtta
    14101 tgccgatata aagcacgcga tccgcatcgc ccagattgac cgtaaaggta tccagcgcat
    14161 agaagaccgg ggagggcacg acgcgttgct gcgctttgtc ttcagcggca acctgatgcg
    14221 aatgccagta gctgtaacct gcgctggcac aggccgcgag ggtaatgaat accagaatcg
    14281 ggatccaaag cgatcgcttg cttttcttgc ttatcgcgta atcagtcatg tgttgcggtc
    14341 ttcctgtgtc gctactgctt atcggctaat tatcctgcgt cttgagcgca acaaagcggg
    14401 gaaaagacgc ggattacggt gctacctctg acgttaggcg aaaatatcaa cgccgctgtt
    14461 gcctgttaca cgcccttgta aagagacggg aaccggaagc gtatcgtcgt cttcccccgc
    14521 cagaggttca tggtttgctg tgcgttggct ttgctgttgc tgggaagcgg cctgctgctg
    14581 accactaaag ctttcgccac tgatgttgct ttgccctaac tgaatgccac tttcggccag
    14641 ctgcgtacgc agtaccggca gcgctgcttc cagggcggcg cgtacgtgct gatgcggcga
    14701 aaccatctgg atttgcgcct ggttatcatc cactttgagg gagatttgca cttcacctaa
    14761 atcctgtggg tgcagacgca actctgcact ttgttgcccc tggcgggtga acagcgaaat
    14821 atgctggctt aatgattgtt gccattcgtg agaacccagc ggtgcactca aaacaggcgc
    14881 ggcgacggtg ggtagtggct gtgtctggtg tggagtgatt agcgggctgg cggcagcggt
    14941 caccggcgaa ggcgtgctga tgatttccgc tttactctgg gcttctgcta ccagcggtgt
    15001 taatggctga gctggtgtgc cgggggcatc atcaggctgt gctgttgtga gttgcgcaga
    15061 agtcagtttt gtgaagagcg ttggtttctc tgccggtaac acggttgacg gcacatcagt
    15121 cactttgggc gtattgtcaa aacccggcaa catcgcaaaa agggcgctca ggcttgcggt
    15181 gacgtcttca ttcagatcat ccgctttttc gtcttttgtc gtatttttgt cagccgccgc
    15241 agccaacgtc atcgtctgag cggtggttaa cggtgttgat gtggattgtt cgtcgttgat
    15301 gacaggcagt gtttcatcca cggggatcag taaatccgct tggtgcgcgt cggaaacaat
    15361 atcgctgacc agcggctcgc cttttgtcgt gggcttatct gttgccacca gcagctgggg
    15421 ggccgctttg tcggtagttg cctcgcctgc taatgcttcg cttaacaacg cgagaaaatc
    15481 ttgtgcagca tcgctggctt tgccgccagg caatgtggtg gtgtcaacgt cggcggtaat
    15541 caagggcgct aagcgaatca ttcaggtttc ctcatggcgg cgcgctgggc gaactcatcc
    15601 atctttttct gatcgaggcg gttttctgca agcagtgccg ccgtggattg ccgttcctgc
    15661 agtgtctgcc aggcctgcaa acgttgtttc ttttctcgcc agctgttcag ggcaatgtca
    15721 actttctgcg tccactgatt aagttgctgg cgatgctgag taatggcttt ttccagcgtc
    15781 tggataaact gctgatagtt gatccagcgg ttgctggtca tcccggcact catatcgcta
    15841 ttgaggttat tgcgatattc attctgataa tcgatcagca ttttgagctg ttcttccgca
    15901 tgctgacatc cgcgacgcat ttcacccagc aggcgcgcgg catcctctac ctctttttct
    15961 gccagatctt tcagggtcgc cagcgcgcca tgttctgcca tctgccgtta tctcctgggt
    16021 tatgacactg tcgggaaaat acgctccagc ccctggagag acgcttccca gtccgcgcgt
    16081 tcaaaaatgc cttgttgcaa atagccctcc agctgcggcc acagggcgat ggctttatcg
    16141 agcatcggat cgctgccttt ggcatacgca ccgacgctaa ccagatcgcg gttacgctga
    16201 aaactcgaca acagctgttt gaaggtgcgt actcgcgcat aatgttgctc gctgatcaac
    16261 gccgtcattg cgcggctgat cgacgcttca atatcgatag ccggatagtg cccggcttcc
    16321 gccagtcggc gagacagcac aatgtgaccg tcgaggattg cccgcgcgga gtcggcaatg
    16381 gggtcctgct ggtcatcgcc ctcggtgagc acggtataaa acgcggtaat cgatccgccg
    16441 ccgctaatgc catttccggc acgctcgacc agtgccggta atttggcaaa caccgacggt
    16501 ggatagcctt tagtggcagg tggttcgcca atcgccagcg caatctcacg ctgggccatc
    16561 gcgtagcggg tgagggagtc cataatcagc aacacatgct gaccacgatc gcgaaaatct
    16621 tcggcaatgc gtgtggcata ggcggcaccc tgcattcgca ggagcggaga aacatccgcc
    16681 ggagcggcaa tcaccacgga gcgtgcacgc ccttcggcac cgaggatatt ctcaataaaa
    16741 tcttttactt cgcgcccacg ttcaccaatc aaacccacga caatgacatc ggcgcgggtg
    16801 taacgtgcca tcatccccag cagcacactt ttaccaacgc ccgacccggc aaacagcccc
    16861 atacgctgcc cgcgcccgac ggtaagcagg gcattgattg ggcgcacgcc ggtgtccagc
    16921 acatgttcaa tcggtgtacg ttgcaacggg ttaaatggcg gggtaatcag cgcaccggtt
    16981 tccgtcgtat cggggaaggg caggccatcg agcggtttac cgctgccatc cagaacgcga
    17041 cctaataacg ccggaccgag cggcaactgc ttgccgcttt gcagtccttc agtcgaaatg
    17101 tttttggcat aaacacgcgc gccgggcagg acaccttcca cttcctccag cggcattaaa
    17161 aacagccgtt gaccgttaaa gccaacgact tcgctttcta cttcgtgggt ttcgctgccg
    17221 ttctggcgtt caatgacaca ggtt
//
LOCUS       DAYPHN010000103        17217 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000103 DAYPHN010000000
VERSION     DAYPHN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.103"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(44..1072)
                     /gene="epmB"
                     /locus_tag="ACG0BW_003184"
     CDS             complement(44..1072)
                     /gene="epmB"
                     /locus_tag="ACG0BW_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFK7260111.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVEHFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1114..1680
                     /gene="efp"
                     /locus_tag="ACG0BW_003185"
     CDS             1114..1680
                     /gene="efp"
                     /locus_tag="ACG0BW_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFK7260112.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1732..1857
                     /gene="ecnA"
                     /locus_tag="ACG0BW_003186"
     CDS             1732..1857
                     /gene="ecnA"
                     /locus_tag="ACG0BW_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFK7260113.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            1968..2114
                     /gene="ecnB"
                     /locus_tag="ACG0BW_003187"
     CDS             1968..2114
                     /gene="ecnB"
                     /locus_tag="ACG0BW_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFK7260114.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2289..2606
                     /gene="sugE"
                     /locus_tag="ACG0BW_003188"
     CDS             2289..2606
                     /gene="sugE"
                     /locus_tag="ACG0BW_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFK7260115.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2603..3136)
                     /gene="blc"
                     /locus_tag="ACG0BW_003189"
     CDS             complement(2603..3136)
                     /gene="blc"
                     /locus_tag="ACG0BW_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFK7260116.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3225..4358)
                     /locus_tag="ACG0BW_003190"
     CDS             complement(3225..4358)
                     /locus_tag="ACG0BW_003190"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFK7260117.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVCASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            complement(4421..4780)
                     /gene="frdD"
                     /locus_tag="ACG0BW_003191"
     CDS             complement(4421..4780)
                     /gene="frdD"
                     /locus_tag="ACG0BW_003191"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFK7260118.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4791..5186)
                     /gene="frdC"
                     /locus_tag="ACG0BW_003192"
     CDS             complement(4791..5186)
                     /gene="frdC"
                     /locus_tag="ACG0BW_003192"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFK7260119.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPESWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5197..5931)
                     /gene="frdB"
                     /locus_tag="ACG0BW_003193"
     CDS             complement(5197..5931)
                     /gene="frdB"
                     /locus_tag="ACG0BW_003193"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFK7260120.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5924..7732)
                     /gene="frdA"
                     /locus_tag="ACG0BW_003194"
     CDS             complement(5924..7732)
                     /gene="frdA"
                     /locus_tag="ACG0BW_003194"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFK7260121.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8057..9034
                     /gene="epmA"
                     /locus_tag="ACG0BW_003195"
     CDS             8057..9034
                     /gene="epmA"
                     /locus_tag="ACG0BW_003195"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFK7260122.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9253..10755
                     /gene="yjeM"
                     /locus_tag="ACG0BW_003196"
     CDS             9253..10755
                     /gene="yjeM"
                     /locus_tag="ACG0BW_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFK7260123.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(10906..14229)
                     /gene="mscM"
                     /locus_tag="ACG0BW_003197"
     CDS             complement(10906..14229)
                     /gene="mscM"
                     /locus_tag="ACG0BW_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFK7260124.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14251..15219)
                     /gene="asd"
                     /locus_tag="ACG0BW_003198"
     CDS             complement(14251..15219)
                     /gene="asd"
                     /locus_tag="ACG0BW_003198"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFK7260125.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15316..16368)
                     /gene="rsgA"
                     /locus_tag="ACG0BW_003199"
     CDS             complement(15316..16368)
                     /gene="rsgA"
                     /locus_tag="ACG0BW_003199"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFK7260126.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16463..17008
                     /gene="orn"
                     /locus_tag="ACG0BW_003200"
     CDS             16463..17008
                     /gene="orn"
                     /locus_tag="ACG0BW_003200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFK7260127.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         3961 a         4447 c         4524 g         4285 t
ORIGIN      
        1 aggttatgca acgtgcaaag attaaacttc cctgtttaat cagttactgc tggcgtagct
       61 ggagatccag cggcgttttg ctgggttcgc cgccaatttc tcgcgccagt ttaggcacca
      121 gatatcccga caccagtgtc agcaactcac gcataatctg ccgtgcttcg tcatcactca
      181 ccataaaatg cgccgcgccc tgtactttat cgagcacatg caggtaatac ggcatcacgc
      241 ccgcatcgaa caacgcatta ctcaggtttg ccagcgtttg cgcgttatcg ttcacaccac
      301 gtaacagaac gctctggttc agcaaagtaa cgcctacccg gcgcaactta gccatcgcct
      361 gacggaatgt ttcatctacc tcattggcat ggttgatgtg attcaccagc aagatttgca
      421 gcgtagaatg tgaaaagtgt tcaaccagcg catctgtgat gcgtgccggg atcacaatcg
      481 gcagacggct gtgaatccgc agacgtttga tatgcgggat ggcttccagt tgtgtgagca
      541 accagtccag ctcgtgatct ttcgccatca gcggatcgcc gccggagaaa atcatctcgt
      601 ccagttccgg atgcgcagca acatactcaa gtgcagtttg ccagttacgc ttgttgccct
      661 gattttcggc ataggggaag tggcgacgga agcaatagcg gcaatttacc gcgcagccgc
      721 ctttgaccag caaaagcgcc cggttgtggt atttatgcaa caaacctggc actacgctgt
      781 gctgttcttc cagcgggtcg gtggagaatc cgggcgcgac gacaaactca tcttgcgagg
      841 taattacctg acgcaaaagc ggatcgttcg gattgccttt ctccatgcga tcgataaatg
      901 agcggggcac acgcagggca aacagctttt tggcgctgcg tccggccaac agtttttcgt
      961 ccgcgtctat attcaaaaga cgcagaagtt catcaggatc ggtcacaaca tcggcaagtt
     1021 gcgttaacca atcttctctg gatggggtat ttagggttac aatatgcgcc attttgtggc
     1081 ttagctacca attaacaaat ttcagagggc cttatggcaa cgtactatag caacgatttt
     1141 cgtgctggtc ttaaaatcat gttagacggc gaaccttacg cggttgaagc gagtgaattc
     1201 gtaaaaccgg gtaaaggcca ggcatttgct cgcgttaaac tgcgtcgtct gctgaccggt
     1261 actcgcgtag aaaaaacctt caaatctact gattccgctg aaggcgctga tgttgtcgat
     1321 atgaacctga cttacctgta caacgacggt gagttctggc acttcatgaa caacgaaact
     1381 ttcgagcagc tgtctgctga tgcaaaagca attggcgaca acgctaaatg gctgctggat
     1441 caggcagagt gtatcgtaac tctgtggaat ggtcagccga tctccgttac tccgccgaac
     1501 ttcgttgaac tggaaatcgt ggataccgat ccgggcctga aaggtgatac cgcaggtact
     1561 ggcggcaaac cggctaccct gtctactggc gctgtggtta aagttccgct gtttgtacaa
     1621 atcggcgaag tcatcaaagt ggatacccgc tctggtgaat acgtctctcg cgtgaagtaa
     1681 tgcggttgtg gtgcggcctg caggctgcac catcacttat tcaggtcaga gatgatgaaa
     1741 cgccttatcg ttcttgtttt gcttgccagt gcgctgctca cgggctgtaa caccgctcgc
     1801 ggttttggtg aagacattaa acatctcggt aattccatct cccgcgctgc cagctaattt
     1861 ttcttctctt ccgaaaaatc atcagatttc catcattttt ggtgatgttg tctattatta
     1921 atttgctata ggcaaacata aataacatta cctaaaagga agacgttatg gtgaagaaga
     1981 caattgcagc gatcttttct gttctggtgc tttcaacagt attaactgcc tgcaacacca
     2041 cgcgtggcgt tggtgaagac atttctgatg gcggtaacgc gatttctggc gcagcaacga
     2101 aagcgcagca gtaagcaata acggtacgac agttatgtcg tgccgtttgt ttttttgcga
     2161 tagtcacaaa ggtaatagtt gaaattcccc tgccacctgg caaaatatcc gttcaaccat
     2221 cagctttgca ggacgacctg caaacgcctc ttttcaccgg ggacggcccc aattctccgg
     2281 agcctgatat gtcctggatt atcttagtta ttgctggtct gctggaagtg gtatgggccg
     2341 ttggcctgaa atatacccac ggctttagtc gtttgacgcc aagtgttatt actgtgacgg
     2401 cgatgattgt cagtatggcg ctacttgcct gggcgatgaa atcgttacca gtagggacgg
     2461 cttatgccgt gtggacgggt attggcgcag tcggcgcggc catcaccggc attgtgctgc
     2521 tcggcgagtc cgctaacccg atgcgcctgg cgagtctggc gttaatcgta ttggggatta
     2581 ttggtctgaa actcagcact cactaactac cgggctgctg tacccaaata aacttactga
     2641 catcaaaccc ttcccgggtc gcgactgcca gcatctcctg tttcacttcg tcagaaatgg
     2701 ttggcgtgcg ggagagtatc cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc
     2761 ggtattcccg atcgagtgca ataacgttat aaccgccata gaaaggacca aagaatgaca
     2821 ctttcagcgc agcgcgagtt ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc
     2881 acatttctct gtcagggtta tagcctttat taatgacatt caggccgccg tcatcccgca
     2941 ggctgtatgt tgcggtgact ttttccagtc cacgttcaaa gcggtgatca aaacgggcaa
     3001 tctcatacca ggtaccgaga tagcgtttag catcgaaatt attcactact gtcacgccac
     3061 ggggcggcgt aggagaactg caggcaacga ccagaaatgc agctgtcgct gcggcaacga
     3121 gagggagcag gcgcataaat gtttccttac tggttttttt ctaagtgtag atgacagcaa
     3181 ggaaaagcgg agaaaaggtc caaaaattcg gacccgatga aattttactg tagagcgttg
     3241 agaatctgcc aggcggcagt gactctcgct ggattgggat agtttttgtt agccagcatc
     3301 acgataccca gctctttttc tggaataaac gcaacatagc taccaaatcc gccggtcgcc
     3361 cccgttttat gtacccatga tgcgcatact gcaggagttg ggggcgtaat cgcttttacg
     3421 gggcgtgctg ccagtgcaat tttattgtca ctgccgttaa tgatgatgtc aggatttacc
     3481 ggccagtcca gcatttccca gcccaggccc tgatacatat cgccggtttg ccagtagcga
     3541 gattgtgcca gttgtatccc ttgttgaagc gttttctcag tgatatcaag gggttttaaa
     3601 ttgctttgca cccagcgggc catatcttca atggtcgact tcacaccata agcttcagca
     3661 tctaacgccc caggcgaaac atgcactgcc ttaccttcgc gatatcccca ggcgtaattc
     3721 ttttcttctg cggacggcac attaatccac gtatgagtga gtttgagtgg ctggaagaca
     3781 cgagtttgca tcgcctgctc aaaactcaaa ccagacggct tcacagccag tgcgccgaac
     3841 aaaccgatac tggagttggc atacagacgt tgtgttcctg gagcccatgc aggttgccag
     3901 ttttgataga agcgcagcaa gtcgcttgag gatttaacgt catccggcac ctgcaatggc
     3961 agaccgccag cagtgtaggt tgcgagatgt aatagtgtga tcccattcca ctgtttagcg
     4021 gtaagttcag gccagtattt tgttgcggga tcgcttaact tgatttcccc tcgagcaata
     4081 gcgtcgccac caagcacgcc cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt
     4141 tgctgtgtga cgggctgctt tttggcgatg tccgcatagc cccaggtaaa gtaataaggt
     4201 ttaccctgat aaattaccgc caccgccata ccggggatct tttgttgctc tataagcggg
     4261 gttattgtgc gatgcacaat atcgttgatt tgttgagggg cagcaaatgt ggagcaagag
     4321 gcggtaatta ataaggcgca gagcgtcatt ttgaacatag ggtctggttt ccatacaaaa
     4381 cggcccgcca taggcgggcc ggatttacat tggcgatgcg ttagattgta acgacaccaa
     4441 tcagcgtgac aactgtcagg atagcagcca gaccgtagaa aacccatttg cccgcaggta
     4501 cgtggatttt cagatcgtgc atcgcgtggt gcatacggtg taaaccacac cacagcggca
     4561 gaacgatcat caggaacagg aatacgcgac caatgaagct ctgcgcgaac gccagaacgc
     4621 gctcgtagct cagtgcatca cccggaaaca gccccagcgg cagcagaata cccaccagca
     4681 ggatcatcac cggcgcaata attgcgctcc acataccacc ggccccgaag aggccccaga
     4741 ataccggttc gtcagaacgc tttggatttg gattaatcat ctcaggctcc ttaccagtac
     4801 agggcaacaa acaggattac gatggtggca accacagtta ccgcccagag acttttgata
     4861 attggctctg gtcccatttt ttcgtctttt acaatgatat tggccgcttt cggtgccagt
     4921 tcaaaccagg ttttggtgtg cagcagagct gccgccagag tgatcaggtt aatgatcaca
     4981 ataaccgggt tttgtaaaaa gtcgacgaat cccgcccagc tttccgggcc atttttcagg
     5041 gcaaacagcc cgaaaatcag ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg
     5101 cgcagcatgt aaaagcgata aaacggcaat tttttccacc aggtggacgt cattggccgt
     5161 acatacggtt tacgtttagt cgtcatgttg cactccttag cgtggtttca gggtcgcgat
     5221 aagaaaatct ttcgaacttt ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg
     5281 tttcgggcag acttcggagc agtagcccac gaaagtacag ctccatacgc cgttctggct
     5341 gttcaactgc gccatacgct ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg
     5401 cgccagcgta atggcagccg gaccgatgaa ctcggggttc aggccaaact gcgggcacgc
     5461 ggcgtagcac agaccacagt tgatgcaacc ggagaactgg tgatacttgg ccatctgcgc
     5521 cggggtctgg atgttagtac cctgatccgc ggtgcgggag ttgccgatga tgtacggttt
     5581 gatcgcttcc agactttcga tgaagtgggt catatcgacc accagatcgc gttcaatcgg
     5641 gaagttagct aacgcttcaa ccttcatacc gtcggtgtaa tcacgcagga aggttttaca
     5701 tgccagtttt ggcacgttgt taaccatcat gccgcaggaa ccacaaatcg ccatacggca
     5761 ggaccagcgg tagctcaggt ccggtgccag gttgtctttg atgtagccca gcgcatccag
     5821 taatgaggta gttgcgtcat aaggcacttc atagaatgcg ctatgcggtg cggtatcgac
     5881 ttccgggtta tagcgcacca cctcaatttt caggtttttc atctcagcca ttcgccttct
     5941 ccttcttatt ggctgcttcc gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt
     6001 tagctggcgg cagcgtagta atcttcacgt cgctgtactc caggcgagtt gtgccatcag
     6061 catcgcggaa ggcgagggtg tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt
     6121 cgtccagacg ctggtgcgcg ccgcgggact ctttacgtgc cattgcggag tgcgccatac
     6181 attcagcaac gttcagaccg tggcccagtt caatggtgta gagcaggtcg gtgttgaaca
     6241 cgctggaggt gtcggtgatg cgcacgcgct tgaagcgttc ctgcagctca gccagcttgt
     6301 cgatggtttt ctgcatcagt tccggcgtac ggtagatacc gcaaccttct tccatagcca
     6361 ggcccatttc gtcgcggatc ttcgcccagt tttcgccgcc atcctggtta accagatctt
     6421 tcagacgttg ttcaacgcca gctgcctgcg cttcaattgc cgcttcgttg ccattaccag
     6481 cagttgctgc acgctctgtc gcttgctcac cggccagacg accgaagacc accagttccg
     6541 ccagggagtt ggagcccaga cggtttgcac cgtgcagacc aacagaggaa cattcaccca
     6601 cggcgaacag acctttaatg cgggtttcac agttctgatc ggtttcgata ccgcccatgg
     6661 tgtagtgtgc ggtcggacgt accggaatcg gttctttaac cggatcgacg ccaacgtacg
     6721 ctttcgccag ttcgcagatg aacggcagac gttcatgcag ttttttctcg ccgaggtgac
     6781 gcaggtcgag ataaaccaca tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt
     6841 cgtgccagaa ggcctgagaa actttgtcgc gtggacccag ttccatatat ttgtttttcg
     6901 gctcgcccag cggagtttcc gggcccatgc cgtaatcttg cagataacgg tagccatttt
     6961 tgttgaccag aataccgcct tcaccgcggc agccttcggt catcaggata ccggaacctg
     7021 gcagaccggt tgggtgatac tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta
     7081 gcgccatacc cataccgtca ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc
     7141 gacccgcacc gccggtagcc ataacgaccg cgttagcacg gatctgcacc agcgtgcctt
     7201 ccatcatgtt cattgctacc aggccgcgaa catgaccatc atcaaccaga atatccagca
     7261 cgaaatgttc gtcaaaacgc tggatctgcg ggaattgcag agaggtctgg aacagcgtgt
     7321 gtagcatatg gaagccggtc ttatcggcgg cgaaccaggt acgctcgatt ttcatgccgc
     7381 cgaagcgacg tacgttgacg ctaccatccg ggcgacggct ccatgggcat ccccacagtt
     7441 ccagttgggt catttcggtt gggcagtggt ggacgaaata atccacgaca tcctgctcac
     7501 acaaccagtc gccacccgct actgtatcgt gaaagtgata ttcgaagctg tcatgatcct
     7561 gcgcgacagc ggcggagccc ccttctgcag caacggtatg gctacgcatc gggtatactt
     7621 ttgagattag tgcgattttt gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta
     7681 atcccgcgcc accggcgcct acaatggcaa gatcggcttg aaaggtttgc acgacattcc
     7741 tccagattgt ttttatccca cagccccgta cttcagggta agtgcctgaa agttacggtc
     7801 tgcgaacgct attccactgc tcctttatag gtacaacagt atagtctgat ggataagtct
     7861 gaaatttgac gagatcgatt tttttagtgc gcaagagggc taaattatca ctgaagatga
     7921 ttaatttaat tactaaacca tcagatcgtg ctttttttag tcactgccca cccttgtttg
     7981 attcctgcgt aaaatttgtc tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa
     8041 aactggagat ttaactatga gcgaaacggc atcctggcag ccgagcgcat ccatccctaa
     8101 cttattaaaa cgcgcggcga ttatggcgga gatccgtcgt ttctttgccg atcgtggagt
     8161 gctggaggtg gagacgcctt gtatgagcca ggcgacggta accgatattc atttggtccc
     8221 gtttgagaca cgtttcgttg gccccgggca ttcgcagggg atgaatctct ggttaatgac
     8281 cagcccggaa taccatatga aacgcctgct ggttgctggt tgtgggccgg tattccagct
     8341 gtgccgcagc ttccgtaatg aagagatggg gcgttatcac aaccctgagt tcactatgct
     8401 tgagtggtat cgaccgcact atgatatgta ccggttgatg aacgaggtgg acgatctctt
     8461 acaacaagtg ctggactgcc cggcagcaga aagcctttct tatcaacaag ctttcttgcg
     8521 ttatctggaa attgacccgc tctctgccga caaaacgcaa ctgcgggaag tggcagcgaa
     8581 actggattta agcaacgttg ctgataccga agaagaccgc gacacgttgc tacaattgct
     8641 gtttaccttt ggcgtagagc caaatattgg caaagaaaaa ccgacctttg tgtaccactt
     8701 tccagccagc caggcatcac tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg
     8761 ctttgaggtt tattataaag gtattgagct ggcgaatggt ttccatgaat tgacggatgc
     8821 ccgtgagcag caacaacgtt ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc
     8881 gcagcacccc attgaccaga atctgattga agccttgaaa gtcggtatgc ctgactgttc
     8941 cggcgtggca ttaggtgttg atcgtctggt gatgttggcg ctgggcgcgg agacactggc
     9001 tgaagtcatc gcctttagcg ttgaccgggc ataattctga aaaattactg aattaaccgc
     9061 gaagaatggc gtgataagcg ccattctgta gcatatgttt tcatttttcc gctaaaggcc
     9121 acaatttaag actggatatt tgatatcatc caggtatcaa ttctgtattg gtttttacgc
     9181 tgcccgctca atgttgggca gtgcagttca gtgggcgcgt tttatacacg cgctgaaatg
     9241 aaggatggtt tcatgcctca cacgataaaa aagatgagtc tgataggact catattgatg
     9301 atctttactt ccgtatttgg atttgccaat agcccatcgg cttattactt aatgggttat
     9361 agtgcgattc ccttttatat attttctgca ttgttattct ttattccatt cgccttaatg
     9421 atggctgaaa tgggagctgc ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat
     9481 aatagtgtcg gaccacgttt tgccttcatt ggtacgttta tgtggttttc ctcttatatc
     9541 atctggatgg tgagtacctc cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt
     9601 agcgacatga cccagcactg gcatattgcc ggactggagc ctacgcaggt ggttggtctg
     9661 ctggcagtgg catggatgat tctggtcacc gtcgttgctt caaaggggat taataaaatt
     9721 gcccgcatta ctgcggtggg cggtattgca gtaatgtgtc tgaatttagt attgctgtta
     9781 gtaagcatta ctattttgtt attaaatggt gggcatttcg cgcaggatat taatttcctt
     9841 gcatcaccga acccaggtta tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct
     9901 atttttgcct atggcggaat tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca
     9961 gaaaagaact ttgccaaagg tattgttttt gccgctattg ttatttcaat cggttattcg
    10021 ctggcaatat ttttatgggg cgtcagcaca aactggcagc aggtattaag taatggttcc
    10081 gttaacctcg gcaatattac ctatgtgctg atgaagagcc tcggggtgac gctgggtaac
    10141 gcactgcatt tgtcaccaga agcgtcattg tcgctgggcg tatggtttgc gcgtattacc
    10201 ggactttcga tgttcctcgc ttataccggt gcgttcttta cgctttgcta ttcaccgctg
    10261 aaagccatca tccagggaac gccgaaagca ttgtggccgg aaccgatgac gcgcctgaat
    10321 gcgatgggga tgccgtctat cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc
    10381 ctgctggttt cgtttggtgg cggtaccgca tcggcgttct ttaacaagct gacgctgatg
    10441 gcgaacgtgt ctatgacgct tccttacttg ttcctcgcgc tggctttccc attctttaaa
    10501 gcacgtcagg atctcgacag accgtttgtg attttcaaaa cgcgtatgtc ggcaatgatt
    10561 gcgacggtgg ttgtcgttct ggtggtgaca tttgcgaacg tcttcaccat tattcagcct
    10621 gtggttgaag ccggagactg ggacagcaca ttgtggatga ttggcggccc tgtcttcttc
    10681 tcgctgttag cgatggcgat ttaccagaac tattgcagtc gcatggcgaa taaacctgag
    10741 ttagctctcg actgatccta tgaaatcctg caactcatcg tctctccgac gatgagttgt
    10801 caggaagtaa tgcgtatgga tgatactctc cagggccggt gataactgaa gatgatgtta
    10861 agctgctaaa tgagccacgt tgacatcatt tggcagcttc atgcattaca aacttcccgc
    10921 ctgacgacct ttgcccgcag acgtcagcgt tctccccgtt tgtttaccgt tgaggctttc
    10981 cagacgcatc tggaagggcg ggaatggcat atcgataccg tgggcgtgga agccagccag
    11041 aatcagctgg tggatctcat ggcgtagcgg catacggtga cccatctcag cggcgtaaat
    11101 acgcagctcg aaaatctgaa tcccctgttg cagatccacc aggaagactt ccggtgccgg
    11161 gttgtcgatc accagcgagc agcgacgcgc tgcggtgagc aggatttccg tcacttcttc
    11221 actgttggca tcggcagggg ccggaatcgt caacaccacg cgcgtgaccg agtcagagag
    11281 cgaccagttg ataaactgct cggtaataaa tgccttgttc ggcacgatta tctctttacg
    11341 gtcccagtcg ctgatggtgg tggcacgggt gttaattttc gtcacgctac cagtaagatc
    11401 gcggatcgtt accgtatcgc caatgcgaat cggtttttcg aacaggatga tcaggccaga
    11461 gataaagttg gcgaaaattt cctgcaaacc aaaaccgaga ccaacaccga gcgcggcaac
    11521 cagccactgc aatttcgacc actcaatacc aatcattgag aagccgacca gcccgccaat
    11581 cagcatcagc agatatttgg tgatggtggt gatggcgtag cccgtacccg gcgttaaatc
    11641 caggtgctgc aaaatcgcca gttccagcag cgcgggcagg ttgcgtacca gctgcgtggt
    11701 gatgataaac accagaatgg caatcagcac cgcaccgagg gtaattggct ccaggctttc
    11761 tacgccctgt accgtggaag tgacatccca cagcgaaata ttttcgagga agccgaaagc
    11821 ggaatggatt tctgaccaca gcacaatgac cgaaagcagg gcgatcaaca tcaaaattga
    11881 gcgcaccagc cgtaaggact gcgcactgat ggcatcgaga tcgacttcgc tttcatcgac
    11941 ttcaattgcc ccttccgggc tactgtgatg atgcgcttcc tcttcgccac gcgcacgttg
    12001 cgctaacatc tctgcccggc gatgcttcgc ccggtcaaac gccagcctgc ggcgctggat
    12061 cagcatccag cggcggataa cgtgataaac caccagtagc aaaaaccaga tggcaaccga
    12121 ggtttcaagc ctcgctaaca gcgcctgtgc cgttgccaga taacccaccg ccgacgccag
    12181 aatggcaacc aacggtgcgc caatcatcat gttccacagc atatggttgg taatgttgtc
    12241 accgctgccc tctttgttga gatacagcgg gatcccggcc tttttcagac tgagagtgac
    12301 caccgccagc gcaccgcaaa tgaggataaa acaaagccgt cccagcgaac cggagaattc
    12361 acggtcgtcg aggttatcga acatcatcag cgccataatc agcggcacaa taagcccgat
    12421 gctcatcagg tagtagcgca tcccacggga aacccgttcg cgcggccagc caaaatgagc
    12481 gataaacaag ccgttcgggc gggcaaaggt agcgcaaatc atcactaccc atagcagcgg
    12541 tacggtggct gttacgccat caccaatcgc taccgccagc ggataaggcc acgcctcgcg
    12601 caagccgtaa cccagcgtca tccacagcac cgggagcggt gacgcgacga gaatcgacca
    12661 gaaaagagtg cgcaacgtca gccaaaagtg atcctgagtc actttgccga ctttcgccgc
    12721 cgaacgttca agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac cgaccagaat
    12781 caacgcgcca aacagcggca aaattgtctc tttgctggtc agcatcatca cactggcttt
    12841 gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg cgatctccag
    12901 cggccaggcg atggtcatcg ggcgcacgtc agaggtccag aacagatagc ggtgcgtcgc
    12961 ttcgttcacc tctttcagcg catcctccag ttgcccgttg gagactttca gcttggtcag
    13021 ttccagcagt agcgtgtcgc caccctgcaa caatgagttc agcaactcac gctgagtgcg
    13081 tagctgtgct tccagaatac ggttttgctc ggcggtcagc ggctgaccgt cggcctgatg
    13141 gatttgcctt agcagcggct gtttatttag cagatcctca taacgtaacc gttgcacacg
    13201 caactgcgcc atttcggtat caagctgttg tggttttggc atttccggta gccgtgccac
    13261 ctgcgcccgc aacgcttcac cgagcaggtt ggacgatccc agccattgcg actgttcacg
    13321 cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac gctgttgcga
    13381 ggcaacgaga tccatccgct gcgcctgttg attcagcgcc gccgatagtt cgcggttaat
    13441 tttgaattgc gcgacgatat ctttcggcaa atcggcgctg ttttctgcca gcaattcggt
    13501 actttccagc gcccgctccg cctcaagctg acgttggctg ttcaactgat tacgtaaggc
    13561 ctgcaaatac gcatccagtt gctggctctc tttttccgcc agctctgagc gtaagcgcgc
    13621 taattcctgg cggttattgg cggacagctg cgccagttcc agttcatcga cgagcgcttt
    13681 caggcgtgcg gagtcagact gcaacgcgaa attttgtgcc tgattgagcg gggaattgcc
    13741 ggtaagcgtc cccaggcggc gctcgatttc atttaactga cggcgggcat cggtttgctg
    13801 ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg cgttcctgct cttgctgggc
    13861 ctgacggctt ttatccagca actggctact gacctggaga atttcctgat tcagcgcgtc
    13921 ggtagacatt cccggtgaca cgctgcgcgg ctcgtcacgc atgttgttta attgcgcgcg
    13981 cagagtagcg gagagtttcg gataattatc aatgacttct tgatattgct tgatgcgctc
    14041 aagggaacct ttccgttcct caagcgcatt taaggcagac tggagcgcct ctacgacttc
    14101 cggttgcgcg ggtttcgccg cttttgcctg ctccagttct tgtgagattt gtttgctatc
    14161 gggggccgtc gcggcgtacg ccccccaact gaggcaccag gccatcagaa aagtgataat
    14221 caggcgcacg tcagcgtttc ctttgatgga ttagacctgg tcttttttat cgtcaaccaa
    14281 tgggctggcg tcgtgttctg cttcgatctc ttcagcagga agcggggcgg gttcagcgtc
    14341 tggcgtaaca aaggtttcgg tagatactgc cagcggctgg ccaattttcg tgacagacag
    14401 gctttccagt tgctcaacca gattcacttt acccggtgca aacaggttga taacggtgga
    14461 accgagttta aagcgaccca tttcctggcc tttcagcagt gccacagaac cgtcgttttc
    14521 cccggcaggc caggtccagc gcttgatgat gccttcgcgc ggcggcgtaa tggtgcccgc
    14581 ccagaccgtc tcaatgctgc ctacaatcgt cgcaccgacc agaatctgcg ccattgggcc
    14641 aaattcggta tcgaacaggc agatcacgcg ttcgttacgg gcaaacagat tcggcacgtt
    14701 ctgagcggtg agatggttaa cggagaagag atcgcccggc acgtagatca tctcacgcag
    14761 aataccgttg cacggcatgt gtacgcggtg gtagtcacgt ggggagaggt aagtggtcac
    14821 aaacgtaccg ttacggaaga gatccgccat cagatagttg cctgccagca gggcttcgag
    14881 gctgtagttg tggcctttgg cttgcaggat tttatcttct tcgattttac ccaactggct
    14941 gataacgcca tcggcaggca taaccagtac attcggatcg gtatcgattg ggcgtacttc
    15001 gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg tatccggctt
    15061 ttgcgcctct ttcatgtcga ccttgtagta tttaacgaac agatcgataa ccagttttgt
    15121 cagccatcct gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc atagtttcgg
    15181 cagaatgtac tgtagcgaaa gtttaaatga atttaacaag gtagcctcca ggccattgtt
    15241 ttgtcgttcc tgatccggcc tacatgccgg atcctgaaaa aaaggggacg attctaacga
    15301 cggttagctt aattgtcagt catccgtatc agaaaagttt ttacgcgttt ttacctgcgc
    15361 catgctttcc agaatacggt gatagttttc gaaacgggtt tccgcgattt ttccttcctc
    15421 aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc gatatttaca
    15481 cagacctaag tagtcatgga attcgacaaa gccctgagtg atttgttccg gctccaggtg
    15541 ccagaggccg aactcacgca cacctgggga gtcaatcaca tcaccgccgt gcgggaagtg
    15601 atacagccga gcggcggtcg tggtgtgctg accgagaccc gagttgtcag aaacatcgtt
    15661 ggtcaggatc tctttttgca gccccagcaa cgcgttaagc aggctggatt tgccaacgcc
    15721 agactgcccg gcaaaaatgc tgatgcgtcc ggtcaacgcc tcttccagcg gtttcagccc
    15781 atcctgagta tgactggaaa ccatcaatac gcgataaccg atattgcggt agatatccat
    15841 ctgctcgttg acgaactcca taccttcgtc gtccagcagg tctatcttgt tgagcacaat
    15901 aatcggctca atctgcaagg tttcgcaggc caccaggtaa cggtcgataa tattgagcga
    15961 cagctccggc aaaatggcgg agacaatgac aatctggtcg atgttggcgg caataggttt
    16021 cacgccatcg tagaagtccg ggcgcgttaa cactgaggtg cgctcatgca ccgcttccac
    16081 gatccctttg acatttacgc cttccgccgc cggtttaccc ggacgccaga ctacgcggtc
    16141 gccggtcacc agcgaacgga tggtacggcg aatattgcag cggtgaacgt cgccatctgc
    16201 ggattccaca tcagcgtgca taccaaagcg gctgatgacg ataccttcat caggctcgcc
    16261 aaacagattg tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac gctggtgatt
    16321 ggcgttcacg cggcgctgct ggcctttgga gagtttattt ttactcaatc gtacagactc
    16381 ctggtcgccc ctgatgggca aaacatctat gatacacgca attgtggatc aatatagtca
    16441 ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac aacctgattt ggatcgatct
    16501 tgagatgacc ggtctggatc ccgagcgcga tcgcattatt gagattgcca cgctggtgac
    16561 cgatgccaac ctgaatattc tggcagaagg gccgaccatt gcagtacacc agtctgatga
    16621 acagctggcg ctgatggatg actggaatgt ccgtacccat accgccagcg ggctggtgga
    16681 gcgcgtgaaa gcgagcacga tgggcgatcg ggaagctgaa ctggcaacgc tcgaattttt
    16741 aaaacagtgg gtgcctgcgg gtaaatcgcc gatttgcggt aacagtatcg gccaggatcg
    16801 tcgcttcctg tttaaataca tgccggagct ggaagcgtac ttccactacc gttatcttga
    16861 tgtcagcacg ctgaaagagc tggcgcgccg ctggaagccg gaaattcttg atggttttac
    16921 caagcagggg acgcatcagg cgatggatga tatccgtgag tcggtggcag aactggctta
    16981 ctaccgcgag cattttatca agctgtaaaa tttttcctgg tcacgtaagc gccggaatcc
    17041 acttgcggcg gcgtgatttt gacgctaaat tgaacgtctt gctgataatt tcagcatttg
    17101 agataaaaac tcaaaaaaaa ttttttaggg gggttgcaga gggaaagatt tctcgtataa
    17161 tgcgcctccc gtaacgacgc agaaatgcga aaattacgaa agcaaaatta agtagta
//
LOCUS       DAYPHN010000104        17189 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000104 DAYPHN010000000
VERSION     DAYPHN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.104"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..356)
                     /locus_tag="ACG0BW_003201"
     CDS             complement(<1..356)
                     /locus_tag="ACG0BW_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260128.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERMLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPR"
     gene            complement(364..1074)
                     /gene="yihL"
                     /locus_tag="ACG0BW_003202"
     CDS             complement(364..1074)
                     /gene="yihL"
                     /locus_tag="ACG0BW_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFK7260129.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSVGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1291..3114)
                     /gene="typA"
                     /locus_tag="ACG0BW_003203"
     CDS             complement(1291..3114)
                     /gene="typA"
                     /locus_tag="ACG0BW_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFK7260130.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3487..4896
                     /gene="glnA"
                     /locus_tag="ACG0BW_003204"
     CDS             3487..4896
                     /gene="glnA"
                     /locus_tag="ACG0BW_003204"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFK7260131.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5070..6119
                     /gene="glnL"
                     /locus_tag="ACG0BW_003205"
     CDS             5070..6119
                     /gene="glnL"
                     /locus_tag="ACG0BW_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFK7260132.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6131..7540
                     /gene="glnG"
                     /locus_tag="ACG0BW_003206"
     CDS             6131..7540
                     /gene="glnG"
                     /locus_tag="ACG0BW_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFK7260133.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPSELFESTVAE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7652..7762
                     /gene="yshB"
                     /locus_tag="ACG0BW_003207"
     CDS             7652..7762
                     /gene="yshB"
                     /locus_tag="ACG0BW_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFK7260134.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7952..9325)
                     /gene="hemN"
                     /locus_tag="ACG0BW_003208"
     CDS             complement(7952..9325)
                     /gene="hemN"
                     /locus_tag="ACG0BW_003208"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFK7260135.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDFAPIEQQW
                     DLHFPDYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9514..10023)
                     /gene="yihI"
                     /locus_tag="ACG0BW_003209"
     CDS             complement(9514..10023)
                     /gene="yihI"
                     /locus_tag="ACG0BW_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFK7260136.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(10142..10308)
                     /locus_tag="ACG0BW_003210"
                     /pseudo
     CDS             complement(10142..10308)
                     /locus_tag="ACG0BW_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10605..11237
                     /gene="yihA"
                     /locus_tag="ACG0BW_003211"
     CDS             10605..11237
                     /gene="yihA"
                     /locus_tag="ACG0BW_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFK7260137.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11619..14405)
                     /gene="polA"
                     /locus_tag="ACG0BW_003212"
     CDS             complement(11619..14405)
                     /gene="polA"
                     /locus_tag="ACG0BW_003212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFK7260138.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLENEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIGPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14769..15701
                     /gene="yihG"
                     /locus_tag="ACG0BW_003213"
     CDS             14769..15701
                     /gene="yihG"
                     /locus_tag="ACG0BW_003213"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFK7260139.1"
                     /translation="MANFLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(15742..17172)
                     /gene="yihF"
                     /locus_tag="ACG0BW_003214"
     CDS             complement(15742..17172)
                     /gene="yihF"
                     /locus_tag="ACG0BW_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFK7260140.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHACDMTLSYKNFDKGFFNSHFQMQITFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4088 a         4369 c         4213 g         4519 t
ORIGIN      
        1 taacgtggat aaaaacgtac ggacgatgcc ccagttttca acgcaatggc gacggcttcg
       61 tcaatagatg ccttatctga agcgctggtt tcaatatgaa tatcaaggtt cagacggcgg
      121 gctttatccc caaaggcaga gagttcttta tcgtccatat taccaagaga aaaacgctcg
      181 ccatccagaa catggatttt gacgccacgt agattatttt cacttgcgat atcaagtaaa
      241 tcagcaggca acattctttc cagccgcata tttaaatgaa aggcataagc atgtaaataa
      301 agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt caccataaaa
      361 cacctacaga ttaatgatga ggtctacggc ctcaaatcgc caatattcaa tatctatctg
      421 gacaatattg ccttgctggt cggcgcgatg cttttcaatt aagatcgctg gcatacccac
      481 agaaccgcca agatacttgc tgatatcgcc cggcattctg acgggtttga aaatcaattt
      541 ttttactacc gtctcttttt ggtagcactt ttcccagact gcagaaaatg agtggttttc
      601 aagttgttca ataaaacccg gagcaacttc aggattaata tatgtttcgt gatagaaaac
      661 tttatgtcct tccagcgccc cccagccggt gatgcgatag agttcagttg atggtgtcac
      721 tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta cggtttttct cggtaaaccc
      781 ccaggaaggc tctcgtcctt gctcaattgc ggcccgctga aagctcgctg acagctccgg
      841 actgtaatta aatcgcggct gggtcacgaa ccacccctta cgatccttac ggaagatttt
      901 ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc atacgtttaa tgcccagcag
      961 ttctcccagc tctctttctg aagggagttt tccacccggc gtggtgatgc catctttcaa
     1021 ccaccgatcc agtttctctt ttgcattttc tacggtggat tgattctccg ccattgttta
     1081 atccttttgg tttaaaccag atgaagcatc ttacgcttac gccaaaaaag aaagcaatta
     1141 acggtgtatg actttgcaga taattgtgac aaagctctct attgacgtaa tcacagactt
     1201 ataagggaga tactcgcatt ggtgtgcgct cacgacaggt aaaaaaaaac ctgccagcaa
     1261 tggcaggttt tttatgactg aaaaacgaaa ttaatcgtct ttcggtgcgc ggttggcacg
     1321 gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggttg gagtcacttc
     1381 taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc ggataggcgg
     1441 aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt tcttaccggt
     1501 caggcagttt acagtcaggt cgttagagcg gctgtgaata ccgataatct gaccttcgta
     1561 aacttctgca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga acagcgcgaa
     1621 cgctaccgct ttaccctgac cgttagagat cagtacgccg ttctgacgct gacccacttc
     1681 acccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagaccag taccggaagt
     1741 catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca cgtagtcgag
     1801 acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac gttcgcccag
     1861 cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc agcgtcacgt tttcatacgg
     1921 ctcttgttta cgaccgtcga tttcacggaa gattactttc ggacgggata ccgccagttc
     1981 gaaaccttca cgacgcatgt tttcgataag aacagacagg tgcagttcgc cacgaccaga
     2041 aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt tgtgtaccag
     2101 ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt ctttaccgca
     2161 gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa cggagagtgc
     2221 cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca gttcgcccag
     2281 gcccgtgatc gccacgatat cgccagcttc cgccagatcg gtttcaatac gttccagacc
     2341 gaggtggccc agcactttac cgactttcgc gttacgggtt ttgccttcgc tatcgatgat
     2401 agtgacctgc tggttcggct tcactttacc gcgcttgatg cggccaatgc cgataacgcc
     2461 aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt caaggtcaac
     2521 gtccggcgca ggaacgtggt caacaatcgc ctgatacagc ggggtcatgt cttccgccat
     2581 atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga tcgggaagtc
     2641 cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat ccacaaccca
     2701 atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct tcaggccgta
     2761 agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa atgcgtcaac
     2821 caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac cgaagtcggc
     2881 gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag cggtgttttt
     2941 cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca cgcgctcttg
     3001 ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta ccagggtggt
     3061 tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga tcacaacttt
     3121 gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta caaaacagga
     3181 tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct ttcacggagc
     3241 ataaaaaggg ttatccaaag gtcattgcac caatatggtg cttaatgttt ccattgaagc
     3301 actatattgg tgcaacattc atatcatggt gcagcccttt tgcacgatgt taagcatgat
     3361 aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt ttttacggcg
     3421 acacggccaa aataattgca gatttcgtta ccacgacgac catgaccaat ccaggagagt
     3481 taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt gaagtttgtt
     3541 gatttgcgct tcaccgatac caaaggtaaa gaacagcacg tcactatccc tgctcatcag
     3601 gtgaatgctg aattcttcga agaaggcaaa atgtttgacg gctcctcgat tggcggctgg
     3661 aaaggcatta acgaatccga catggtgctg atgccagacg cgtccaccgc agtgattgac
     3721 ccgttcttcg ccgattctac cctgattatc cgttgcgaca tccttgaacc tggcaccctg
     3781 caaggctatg accgtgaccc gcgctctatt gcgaagcgcg ccgaagatta cctgcgttcc
     3841 actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct gttcgatgac
     3901 atccgtttcg gatcttctat ctccggttcc cacgttgcta tcgacgatat cgaaggcgca
     3961 tggaactcct ccacccaata cgaaggtggt aacaaaggtc accgtccggc agtgaaaggc
     4021 ggttacttcc cggttccgcc ggtagactct gctcaggata ttcgttctga aatgtgtctg
     4081 gtgatggaac agatgggtct ggtggttgaa gcccatcacc acgaagtagc gactgctggt
     4141 cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga aattcagatc
     4201 tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta aaaccgcgac ctttatgcca
     4261 aaaccgatgt tcggtgataa cggctccggt atgcactgcc acatgtctct gtctaaaaac
     4321 ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc gctgtactac
     4381 attggcggcg taatcaaaca cgctaaagcg attaacgccc tggcaaaccc gaccaccaac
     4441 tcttataagc gtctggtccc gggctacgaa gcaccggtaa tgctggctta ctctgcacgc
     4501 aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg tcgtatcgaa
     4561 gtacgtttcc cggatccggc ggctaacccg tacctgtgct ttgctgccct gctgatggcc
     4621 ggtcttgatg gtatcaagaa caagatccat ccgggcgaag ccatggacaa aaacctgtat
     4681 gacctgccgc cagaagaagc gaaagagatc ccacaagttg caggctctct ggaagaagca
     4741 ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt cactgatgaa
     4801 gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg
     4861 catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta ctacccggcg
     4921 caacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
     4981 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
     5041 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
     5101 agatcctcaa ctcgctgatt aacagtattt tgttaatcga tgacaacctg gcgatccatt
     5161 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacac
     5221 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
     5281 aggcggggca aggttttacc gataacgaag tgacgctggt catcgacggg cgctcgcata
     5341 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggcaccga
     5401 tggataacca gcgccgctta agtcaggaac agctacagca cgcccagcag gttgctgccc
     5461 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
     5521 cggcgcagct gctcagcaaa gcattacctg acccgtcact actcgaatat accaaagtta
     5581 ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg tctgttggga ccgcaactgc
     5641 ccggtacgcg cgttaccgaa agtattcata aagtggctga acgcgtggtg acgctggtgt
     5701 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgaccccagc ctgccggaac
     5761 tggcgcacga cccggatcaa attgagcaag tgctcctgaa tattgtgcgc aatgcgctac
     5821 aggcgctggg gccagagggt ggtgaaatca ttctgcgtac ccgcacagct ttccaactga
     5881 ccttacacgg cgagcgttat cggctggcgg cgcgaattga tgtggaagat aacgggccag
     5941 gtattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
     6001 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
     6061 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
     6121 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
     6181 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg agaacggcgc
     6241 ggaggtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
     6301 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc cgatgcttcc
     6361 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
     6421 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gccgtcgcgc tggttgagcg
     6481 cgccatcagt cattaccagg aacagcagca gccgcgtaat gttcagctta acggcccaac
     6541 gaccgatatc atcggcgaag cgccagccat gcaggacgtg ttccgtatta tcggtcggct
     6601 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
     6661 cgctcatgcc ctgcatcgcc acagtccgcg agccaaagcg ccatttatcg cgctgaatat
     6721 ggcggctatc ccgaaggatt tgatcgaatc agaactgttt ggtcacgaga aaggcgcgtt
     6781 tactggcgcg aacaccattc gtcaggggcg ttttgaacag gccgatggcg gcacgttatt
     6841 tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
     6901 agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
     6961 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct
     7021 gttccaccgc ctgaacgtca tccgcgttca tctgccgcca ctgcgtgagc gtcgggaaga
     7081 tattccccgt ctggcacgtc attttttaca ggttgccgcg cgcgagctgg gcgtagaagc
     7141 gaagttgctg catccggaaa ccgaagccgc gctgacgcgt ctggcatggc cgggtaacgt
     7201 acgccagctg gaaaacacct gtcgctggct aacggtgatg gctgccggac aggaagtgtt
     7261 gattcaggat ttgcccagcg aactgtttga gtcaacggtt gcggagagta cttcgcatat
     7321 gcaaccggac agttgggcga cgctgttagc gcagtgggca gacagggcgc tgcgttccgg
     7381 tcatcaaaat ctgctttccg aagcgcagcc tgagctggag cggactttac tgacaaccgc
     7441 attgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa
     7501 cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
     7561 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
     7621 tgatcttgcc cggaaaacgg ggagagtcgt tatgctggaa tcaataatta atctggtatc
     7681 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
     7741 aatgattggg ctgtttagtt gagtttcaga ttgctgatga agtgctcttt gttcatgccg
     7801 gatgcggcgt gaacgcctta tccggcctac aaaagcaata ttttcaacag gttttgtagg
     7861 cctgataagc gtagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt
     7921 tctactttgt aaacgaagcg ccattcacta tttaaatcac ccgagagaac tgctgcatcc
     7981 gcgctttctg gcgcagatag gtatcaaagc acatgcaaat gttgcggata agcaggcgac
     8041 ctttcgccgt cacctgaatc cccttctcat ccacatccac cagcccatct tttgctaacg
     8101 gggcgagcag cttgagatct tcggcaaagt aatcagggaa gtgcaaatcc cactgttgct
     8161 caatgggggc gaaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa
     8221 tacagtcatc acgcgttagc gcaataccac gccacagcgc attgccttgt tcatccactt
     8281 gctgatagta ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca atcatgctga
     8341 tggcggaaac gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt
     8401 tacgatgcag cacgccttca cgctgggcca ccgccagctc gtcatccggg cgagcaaagt
     8461 gatccatacc gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga
     8521 tatcgagttt ttgctgcgga ctcggcaagt cagcatcttt gattttgcgc tgagcagcaa
     8581 aaatggtcgg cagatgcgcg tagttgaaga cactcagacg atcggggttc agctccgcca
     8641 cgcgtttcag ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg taaatcaggt
     8701 cgatgttggt ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca aagatgaact
     8761 cttcatcctg ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca
     8821 tgctcaggcg attaaagccc tcggcgcgta aatgatcgag tacatccagt tcgatttcac
     8881 gcggatcgac ttcgatcgaa atctccgcat cggcattgaa ctggaagttt tcgcgcagca
     8941 gcttcatcag acggctgatt tgcgctttgt tcagatacgt cggcgtacca ccgccccagt
     9001 gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt tcttgctcca
     9061 gcgcgtccag atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc
     9121 agaagtaaca aagcttatgg cagaacggaa tatgtacgta gagagataat ggacgctcag
     9181 gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg
     9241 cggtcgggta cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat
     9301 cccagtcgat ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg
     9361 gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg
     9421 cgacaaccgc tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat
     9481 cagcagaacg ggaaggccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat
     9541 gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt
     9601 ctgcatcaac tcatcaatac gatccagttt ggcatccacc caggattgct cttcggcact
     9661 cagggtttcg cctgcttcca gacgttccag cagcgcatcc agacgctcat ccgtttccag
     9721 taattccaac tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt
     9781 ggtgactttt tcagtcacgc ccaatggaat aggggtttta ctgccaatac gtggatcttt
     9841 cggtgcgttc tggcctttgc tgcctgacgt ggtgttaccg cccgctgcgc ggctgcccgg
     9901 cgcatgacca cgacgttttt tctgacgctt acggtcacga gcttcctgat ccagctcctc
     9961 gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt
    10021 cataatggtt cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga
    10081 aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc
    10141 cttaacgggt cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc attagccgga
    10201 acaatccgtg ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca
    10261 atcctctgtc ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag
    10321 cgtcctcgct ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat
    10381 cagtatttgc acattttcag agttttatat aaaagcaatt aactgattta tatagcttat
    10441 ttgacatgca tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca
    10501 caaagcgtat ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt
    10561 caggtataat cgccgcaaag ctttgactga cggagacgac cgctttgact aatttgaatt
    10621 atcaacagac gcattttgtg atgagtgcgc ctgatattcg ccacttacct tccgataccg
    10681 gaattgaagt ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc
    10741 tgactaacca gaaaagcctg gcgcggacct caaaaacccc agggcgcacc cagcttatca
    10801 acctgtttga agtggctgac ggcaagcgtc tggttgactt gcccggctat ggctacgcgg
    10861 aagtcccgga agagatgaag cgcaaatggc agcgcgcgct cggcgaatac ctcgaaaaac
    10921 gtcagagcct gcaaggtctg gtggtgctaa tggatattcg ccatccgctg aaagatttgg
    10981 atcagcagat gattgagtgg gcggtagaca gcaatatcgc cgtgctggtg ctgctgacca
    11041 aagcggacaa actggcaagc ggcgcacgta aagcgcaatt gaatatggtg cgtgaagcgg
    11101 tgctggcgtt taacggtgat gtgcaggttg aaacgttctc ttcactgaag aaacaaggcg
    11161 tggacaagct gcggcagaaa ctggatacct ggtttagcga gatgcagcct gtagaagaaa
    11221 cgcaggacgg cgaataattt tcttgcctta atgcttgtgc cggatgtggc gtatccggcc
    11281 cgtaaattca atagattaca cttctaccgt tggcctgata aacagggcgc atcaggcatt
    11341 acggagcttt tctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa
    11401 aatattcagc caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct
    11461 ctgtacccta cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa
    11521 ttttttttca gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg
    11581 ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt
    11641 tcgccactcc ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt
    11701 tgatgaatct gcttcgcgac ggcatcgaca tcatctttat gaacttcaaa taccagttca
    11761 tcgtgtacct gcatgatcat acgcacacgc ggttgctcag cctgtagcca cgcatcaacg
    11821 gcaatcatcg cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct
    11881 gcacgttcag ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga
    11941 cggcgtccgt ccagtgtttc aacgtagccc tgctctttcg cctgagcacg ggtgcgttcc
    12001 atatactcca gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct
    12061 ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca
    12121 aagttgatcg ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca
    12181 aacacttctg ccgccgtagc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag
    12241 cctttgtcac gcgagagatg cgccataatg cgcagttcaa tctgcgagta gtccgccgag
    12301 acaatcacat aatcctctgg cccaataaac gcctggcgga tacgacgacc ttcttcgtta
    12361 cgcaccggaa tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttact
    12421 gcctggtgat aagaggtatg tacacgcccg gttttcgggt tgatcatcag cggcagcttg
    12481 tcggtgtagg tcgatttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac
    12541 ggatagtcca gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc
    12601 gttttcttca gcggtttaat tccctgtttt tcaaacagaa tggtttgcaa ctgcttggtg
    12661 gaggaaaggt taaactcctc acctgcaatt tcatgcgctt tcttttccag ctcagccaga
    12721 cgaagagtga gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta
    12781 cgttcaatgc gtgaaagcac cggcaccagc ggcatttcga tattctcgaa gacgttcaac
    12841 ggccctttgt gtttttgcag atccggccac attttcagat gcaactgcaa ggtgacatcc
    12901 gcatcttcgg cggcgtaacg tccggcctct tcgagggcaa tctggttaaa ggtcagttga
    12961 tttttgcctt tacccgcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc
    13021 gcgaggctgt ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg
    13081 gtatcaaacg caatcccacg cagctcaatt ccgtaattcg ccagaatacc gcggtcgtat
    13141 ttcaggtttt gcccgacctt cagcgccttt tcattttcca gcaacggttt tagcaactcg
    13201 agtgcacgct cgcgagagat ttgatcgggc gcatcaagat aatcatgagc aaccggaata
    13261 tatgccgcta cgcctggctc gatagcaaaa gaaagcccga ccaggttggc agagatgtta
    13321 tcgaggctgt cggtttcggt atcaaatgca aatactggcg ctttttccag cttcgcaatc
    13381 cacgctttca gtgtttcttc atcaaggatg gtgacgtagt tgtcataaga aatcaccgtt
    13441 gccgtcactt ctggcgcttc gtctgcaaca ctggtttcct gcggcctggc tgcgggtttc
    13501 gcccctttag cctgtaacca tttgcccgct tcgacatcag cagtccagcg tttgaactca
    13561 tactttttga acagccccaa caactcttcc gccgccggtt gctgcacttc cagttgttca
    13621 caggtcagct ccagttcaac gtcggtttta atcgtcgcca gctggtatga gagataagca
    13681 acttctttgt tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca
    13741 gcaatttttt ctggctcggc atacagcgta tccagtccgc caagaccttg cagcaatgcc
    13801 tgcgcggttt tttcaccgac gcccggtacg ccagggatgt tatcagagga gtcacccatc
    13861 agcgccagga agtcgatgat cagttctggc ggcacgccgt acttattcac cacctcttcc
    13921 gggccgagga tggtattcgt catggtattg ataagcgtaa tatttggcgt caccagctgc
    13981 gccatatctt tatcgccagt gctgatcagc accggacgcc cggctttttc ggcttcccgc
    14041 gccagagtac cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc
    14101 atcgctttaa ccatcgcgtg caagggttcg atttgtgcac gcagatcgtc cggcattggc
    14161 gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca
    14221 aagaccaccg ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg
    14281 acaccataca tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga
    14341 tatgcgcgat aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga
    14401 accataatgt ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggatgaa
    14461 aacatgagtt tttcaccgct tttcgtgaaa atcttttgta gatcttctgg atcgctcgca
    14521 aatcgtcatg tggataactt tgtgcataaa ttatttacat cgcttttaat ttattatcat
    14581 gctttttaca gcatgatgtt tgtctttgtt tttcatgaag ttaaaaataa gaccatccct
    14641 aataattgag atgatgggat tttttctaat gtggatagat atgaattatt tttctcctta
    14701 aggatcatcc gttatttggg tcgttttctg ctaaaatctg cgccttgcgc ttctttagcg
    14761 cactgtctat ggctaacttt ttgaataaat tcattatgac gagaatactc gctgcgataa
    14821 cccttttgct gagtatcgta ttgaccattt tggtcactat tttttgctcc gtcccgatca
    14881 tcattgccgg gattgtaaaa ctattgcttc ctgtgccagt catttggcga aaggtttcgc
    14941 gtttttgtga ttttatgatg tattgctggt gtgaaggtct ggcggtatta ctgcatttga
    15001 acccacacct gcaatgggaa gttcacgggc tggaagggtt aagtaagaaa aactggtatc
    15061 tgcttatttg taaccaccgt agttgggcag atattgtcgt actgtgcgtg ctgtttcgta
    15121 agcacattcc gatgaataaa tatttcctca agcagcagct ggcctgggtg ccttttcttg
    15181 gcctggcatg ctgggcgctg gatatgccgt ttatgaagcg ttattcccgc gcttatttat
    15241 tacgtcatcc ggagagacgt ggtaaagatg ttgaaactac tcggcgttct tgtgaaaagt
    15301 ttcgcctgca tcccaccacc attgttaatt tcgttgaagg ctcccgtttc acgcaagaaa
    15361 aacatcagca aacgcactcc acttttcaaa acttgttgcc accaaaggcg gcaggcattg
    15421 cgatggcgct aaatgtactg ggtaaacaat tcgataaact gcttaatgtt acgctatgtt
    15481 atccggacaa taaccgtcag ccgttcttcg atatgttaag cggtaaactg acgcgaattg
    15541 tcgtacatgt ggatttacag cctattgccg atgagttaca tggcgactac atcaatgaca
    15601 aaagctttaa gcgccatttt cagcaatggc tgaactcact gtggcaggaa aaagaccgac
    15661 ttctgacgtc gttgatgtca tcacagcgtc agaataagta agttttactc gcctctgaat
    15721 gagcagaggc gagtgagtat tttaatgaac aaaacgtccg gcacgagaca taaattcttc
    15781 ttcgctcatt tcctgtccgt taaaaacaac tttacccggt gtataacgca gttgcagcga
    15841 ggcggtattg ttgtcaatcg tgattaactg aaacatctga cctaatgtca tcatcccgct
    15901 gatttgttta tcagcccgct tttgcgcttt ttcagcatcc attccttcag ataaattaag
    15961 ctgttttgcg gtttctgtga cgacattaag cggtaacttc acatcaaaat tgagcgattt
    16021 aatatctttg tttgtggatg atgaggattt ggctgggtcg gcaatactga tatccagatt
    16081 ggcatttagt tcgccgagtg cattcttcca tcttaccggt tgtttaatgg tcggctcact
    16141 tttttgtaat aacggtaaat actctttgaa caatgcagca ttcacttctt gcagagcaac
    16201 ttcatcgttt gctaactcag ggtgtgcagc aagctgcttc tgcatcgcaa tgttatactg
    16261 gatgataaat tggcgcactg ctgagggatc aatagattca gcaatcaatg accactcacc
    16321 actaccgagt tgctgattcc cttttgttaa cttatcaagt tcgtaggtca gattgacatt
    16381 gatataatct ttgtctttaa cgcgatccag cctggttttt gcaacgaaag catcgatttg
    16441 ggcaacgtct tccccgtgat tgataatgtt aatctgatta aatttcgact cgctttcacc
    16501 aaccggaaat ttcttttctt ctaatcgcgc cagagaatca atggtaaaac tttttgccgt
    16561 tactttgttc attggtgata attgcagaac aatatcttcg actttgcctt caatatccag
    16621 gttagacaat gaactaccat caccattaaa agtaaactgg ccctcaccaa aagaaaactt
    16681 tccatactct gcaggggcaa catctaatgt cgtagagaat gagccaccaa acgcgaatcg
    16741 caatgtcgct tccacgggcg atttattttt cgcggcgata aatagcggtt gtgtcagttc
    16801 attattcact aagttcactt ttgccgctgc cagtgcgggg atgacattac cacgcattaa
    16861 cattgtgatg ggcaacggac cgtgctcaac atccacatca aatgcaactt tctggcctgg
    16921 cttgatattg agatcgggtg caccgttatc aaatgttatt tgcatttgaa aatgagaatt
    16981 aaaaaagcct ttgtcaaagt ttttataact taacgtcata tcgcaggcat gttcaccttt
    17041 ctttttagca tcgttaaaat ctttaataaa cttttcgaca ccaggctgaa tttgcgtacc
    17101 tgtgtaccat gtgccaccac cccagattac ggctaacgca acaataacac ctgtagctga
    17161 cttacgtatc atataaatat tccctgata
//
LOCUS       DAYPHN010000105        17068 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000105 DAYPHN010000000
VERSION     DAYPHN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.105"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..257
                     /locus_tag="ACG0BW_003215"
     CDS             <1..257
                     /locus_tag="ACG0BW_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFK7260141.1"
                     /translation="TVTMLPSFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTS
                     DSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(399..725)
                     /gene="ypeC"
                     /locus_tag="ACG0BW_003216"
     CDS             complement(399..725)
                     /gene="ypeC"
                     /locus_tag="ACG0BW_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFK7260142.1"
                     /translation="MFRSLFLAATLMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(840..2096)
                     /gene="yfeO"
                     /locus_tag="ACG0BW_003217"
     CDS             complement(840..2096)
                     /gene="yfeO"
                     /locus_tag="ACG0BW_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFK7260143.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIAILTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALLG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGKM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2300..3265
                     /gene="glk"
                     /locus_tag="ACG0BW_003218"
     CDS             2300..3265
                     /gene="glk"
                     /locus_tag="ACG0BW_003218"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFK7260144.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3485..3811
                     /gene="fryB"
                     /locus_tag="ACG0BW_003219"
     CDS             3485..3811
                     /gene="fryB"
                     /locus_tag="ACG0BW_003219"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFK7260145.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3833..5080
                     /gene="fryC"
                     /locus_tag="ACG0BW_003220"
     CDS             3833..5080
                     /gene="fryC"
                     /locus_tag="ACG0BW_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFK7260146.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5095..6180
                     /gene="ypdF"
                     /locus_tag="ACG0BW_003221"
     CDS             5095..6180
                     /gene="ypdF"
                     /locus_tag="ACG0BW_003221"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFK7260147.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     VSAHILVDSRYYADVEARTQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6180..7217
                     /gene="ypdE"
                     /locus_tag="ACG0BW_003222"
     CDS             6180..7217
                     /gene="ypdE"
                     /locus_tag="ACG0BW_003222"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFK7260148.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGIPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7242..9737
                     /gene="ptsP"
                     /locus_tag="ACG0BW_003223"
     CDS             7242..9737
                     /gene="ptsP"
                     /locus_tag="ACG0BW_003223"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFK7260149.1"
                     /translation="MLTIQFLCPLPNGIHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIITTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9740..10597)
                     /gene="ypdC"
                     /locus_tag="ACG0BW_003224"
     CDS             complement(9740..10597)
                     /gene="ypdC"
                     /locus_tag="ACG0BW_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7260150.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRSDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10610..11344)
                     /gene="ypdB"
                     /locus_tag="ACG0BW_003225"
     CDS             complement(10610..11344)
                     /gene="ypdB"
                     /locus_tag="ACG0BW_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFK7260151.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11359..13056)
                     /gene="ypdA"
                     /locus_tag="ACG0BW_003226"
     CDS             complement(11359..13056)
                     /gene="ypdA"
                     /locus_tag="ACG0BW_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFK7260152.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            13433..14671
                     /gene="alaC"
                     /locus_tag="ACG0BW_003227"
     CDS             13433..14671
                     /gene="alaC"
                     /locus_tag="ACG0BW_003227"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFK7260153.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISHWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFVKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(14736..14807)
                     /gene="ypdK"
                     /locus_tag="ACG0BW_003228"
     CDS             complement(14736..14807)
                     /gene="ypdK"
                     /locus_tag="ACG0BW_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFK7260154.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(15163..16083)
                     /gene="lpxP"
                     /locus_tag="ACG0BW_003229"
     CDS             complement(15163..16083)
                     /gene="lpxP"
                     /locus_tag="ACG0BW_003229"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFK7260155.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAIARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            16436..16678
                     /gene="yfdY"
                     /locus_tag="ACG0BW_003230"
     CDS             16436..16678
                     /gene="yfdY"
                     /locus_tag="ACG0BW_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFK7260156.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(16755..17015)
                     /gene="ypdI"
                     /locus_tag="ACG0BW_003231"
     CDS             complement(16755..17015)
                     /gene="ypdI"
                     /locus_tag="ACG0BW_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFK7260157.1"
                     /translation="MLFSLFLLVSIMACNVFAFSISGGGSEASYKETEKTSAMTTTHS
                     TKLQPSQAILFKMREDAPPLNLTEDITPPFSTKANYLIHPVR"
BASE COUNT         4098 a         4289 c         4568 g         4113 t
ORIGIN      
        1 gtacagtcac tatgttgccg tcatttattg tgattctgat gggattagat ccgacacgga
       61 ttctggttat gagtcaggtg ttgttaagtt ttggtatcgc cctggcgctg gttccactgc
      121 tgattttcac cagtgacagc aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac
      181 agacaggctg ggtgattgtg gtgctggtag tggcgctgaa tatctggtta ctggtgggaa
      241 cggcactggg attgtagttc agctggtgtc gcatctggca ccattggcgg atgcggcgca
      301 aaacgcctta tccgccctac gcgttaaaga gacggtttgt aggcctgata agacgcgcta
      361 gcgtcgcatc aggcattgtg ctccattgac ggacgcgttt aatggcgatg tccatgtccg
      421 cggccttttc cgcgatgatc gtcacgatcg cgccagcctt cacgatagcc acgctcatac
      481 gctttacgct tatcccagcc acgatggtag ccattatcat gacgccacca gcggttctta
      541 cgccattcat aattgcgatg ccagtagtca cggtcgcgcc agtgaccacc gtcccagtaa
      601 ttaccgtaat gatcgcgatc gccaatttgt aatttgattg atggcagtag ggtgatttca
      661 cctgcgtttg ccgcaagcgg ggtaaatgcc atcagggtgg ccgccagaaa cagtgacctg
      721 aacattgtta ttctccttca cgatcgaagc cgtcagcggc ctgttaacgc aatattacgg
      781 ggaggtaaag ccccgtatca tcgccataac tcttaaatca taaaggagag cattttttgc
      841 taaacgttat cgtggggtgg ctgttgcttc ggacgattga ccatcatcat cggcttacct
      901 gctaataaca gccatgccgg aagcatgacg atacagagca gcggtagcaa tgtggtattg
      961 ggtacaacga ctgccgccat aaaaagactt aaccagccat cgcgtgttac caccagcacg
     1021 atgccgagaa tagcgcagga aacggttatt gccgctggaa cggcgggaac gtgctcatgc
     1081 agcatcaacc ctaatgccac cccgacaaac actgccggga agattcgccc accgcgaaag
     1141 ccactggcgg cagcaacgac cagggcggca agtttaatta ccgccagcaa aaagtaatcg
     1201 ctggtgctga aagcctgatt tgccaccatc tgctgcatct catccagccc tttaaacagc
     1261 gaaactggtc cacccataac ccccagaata ccgagaataa atccgccaat acccagcacg
     1321 agcaccggat ttttcatttg atgcatcatc gcgtgcaacc gtggtaagca ccatacggcg
     1381 accatccctg ctgcgatggc aatcgccgcg acaattgcac cgctgagaat atcggtcatt
     1441 tccatttttc cgtaatgcgc aatgggcagt gaaaaatggg gatggaaaaa taatccggta
     1501 gtaagtgcac cagctgctgc cgccattaac ggcgcaaaaa gacgatccca tagcggaact
     1561 tcactactac catttaaggt ttgcgaaaat atcaacgccg ccgcaacagg tgtgccaaac
     1621 agtgcgccga tggtgccggc agaggctaaa atagtccact ccattcggtt gacgcgcggt
     1681 aacagacgag cgccaatggc aaccgcaagg gcgatattga cagtcatgat cggatgttcc
     1741 ggccccaggc tgacgccgcc agcaagaccg agaattaatg ctacgataag tccaagtagc
     1801 gcagagggcg gaaccggtgc gccgatcagc ggttcacagg cggggtctgg tccagcatgc
     1861 ccctggctga aacggataac caaccccacc gcaatacctg ttagcgttaa tatggcgatg
     1921 atccagaaag gtgaatcctg agctatcccc agagttcccg gcagtcgttg ccagagcaaa
     1981 ttctgtaata ccgaggcgat tttcatcacc acaatcagaa taagactgga cgcaatccca
     2041 attgccacgg cggggagcga taataacaac atggttctgg ctcgcggatg gagcatgttc
     2101 tattccttat gcggggtcag atacttagtt tgcccagctt gcaaaaaggc atcgctgcaa
     2161 ttggtgctga aacgataaag taattgtgtg acccagatcg atatttacag ggagcctgcc
     2221 tttccggcgt tgttgttatg cccccaggta tttacagtgt gagaaagaat tattttgact
     2281 ttagcggagc agttgaagaa tgacaaagta tgcattagtc ggtgatgtgg gcggcaccaa
     2341 cgcacgtctt gctctgtgtg atattgccag tggtgaaatc tcgcaggcta agacctattc
     2401 agggcttgat taccccagcc tcgaagcggt cattcgcgtt tatcttgaag aacataaggt
     2461 cgaggtgaaa gacggctgta ttgccatcgc ttgcccaatt accggtgact gggtggcaat
     2521 gaccaaccat acctgggcgt tctcaattgc cgaaatgaaa aagaatctcg gttttagcca
     2581 tctggaaatt attaacgatt ttaccgctgt atcgatggcg atcccgatgc tgaaaaaaga
     2641 gcatctgatt cagtttggtg gcgcagaacc ggtcgaaggt aagcctattg cggtttacgg
     2701 tgccggaacg gggcttgggg ttgcgcatct ggtccatgtc gataagcgtt gggtaagctt
     2761 gccaggcgaa ggcggtcacg ttgattttgc gccgaacagt gaagaagagg ccattatcct
     2821 cgaaatactg cgtgcagaaa ttggtcatgt ttcggcagag cgcgtgcttt ctggcccagg
     2881 actggtgaat ttgtatcgcg cgattgtgaa agccgacaac cgcctgccag aaaatctcaa
     2941 gccaaaagat attaccgaac gtgcgctggc tgacagctgc accgattgcc gccgcgcgtt
     3001 gtcgctgttt tgcgtcatta tgggccgttt tggcggcaat ctggcgctca atctcgggac
     3061 atttggcggc gtgtttattg cgggtggtat cgtgccgcgc ttccttgagt tcttcaaagc
     3121 ctctggtttc cgtgccgcat ttgaagataa agggcgcttt aaagaatatg tccatgatat
     3181 tccggtgtat ctcatcgtcc atgacaatcc gggccttctt ggttccggtg cacatttacg
     3241 ccagacctta ggtcacattc tgtaaatcct tcctgttata tcgggaggta actctcccga
     3301 taatctttta aatcatacag ttaattcaat ttttctttgc gtcctcctca caaggccgac
     3361 ctgcgtcaca cttccgtaca gcgggattaa ttctccagta aatgcattat ttgtctggta
     3421 acggcgattt gttttgcacg ttcataattt cactcgtcaa ctatcactga acgaggaatt
     3481 aacgatgagt aagaaactga ttgccttatg tgcctgccca atgggcctgg ctcacacctt
     3541 tatggccgct caggcgctgg aagaagcggc ggtagaagca ggctatgaag taaaaattga
     3601 aacccagggc gcggacggta tccagaatcg cctgacggcg caggatatcg ccgaagcgac
     3661 tatcatcatc cactccgtgg cggttacccc ggaagataac gaacgtttcg aatcacgcga
     3721 cgtttatgaa atcactttgc aggacgcaat taaaaacgct gcgggcatca tcaaagaaat
     3781 cgaagagatg attgcctctg aacaacagta atattcgaca gggataattg ttatggccat
     3841 taaaaaacgc agtgcaaccg ttgtgcctgg cgcatccggt gcggcagcgg cagttaagaa
     3901 tccgcaggcc tctaaaagca gcttctgggg tgaactcccg cagcatgtga tgtcagggat
     3961 ttcacgcatg gtgccgacct taattatggg cggtgtgatc ctcgctttca gccagcttat
     4021 tgcttatagc tggcttaaaa ttcctgctga tatcggcatc atggatgctc ttaatagcgg
     4081 gaaattctcc ggtttcgacc tttccttact gaaatttgcc tggctgtcgc agtccttcgg
     4141 cggcgtgttg tttggtttcg ccattccgat gtttgccgct tttgtggcga actctatagg
     4201 tggcaaactg gcttttccgg ctggttttat cggcggcttg atgtctaccc agccgacgca
     4261 actgctgaac ttcgatccca gcactatgca atgggcgacc tcttcgccgg tgccgtccac
     4321 cttcattggt gcgctgatta tttctatcgt tgccggttac cttgtgaaat ggatgaacca
     4381 gaaaatccag ttaccggatt tcctgttagc gttcaaaacc acatttttgc taccgattct
     4441 ttccgccatt tttgtcatgc tggcgatgta ctacgtcatc accccctttg gcggctggat
     4501 caacggcggt atccgtaccg tgctgactgc cgcaggtgag aagggtgcgc tgatgtacgc
     4561 aatggggatc gccgcagcga cagcaattga ccttggtggc ccgatcaaca aagcagccgg
     4621 tttcgttgcc ttcagcttta ccactgacca cgtattaccg gtcaccgcac gttctatcgc
     4681 tatcgttatt ccgccgattg gtctgggtct ggcaaccatt attgaccgtc gtttaaccgg
     4741 caaacgtctg ttcaacgctc aactttatcc gcagggtaag accgcgatgt tccttgcctt
     4801 tatggggatt agtgagggtg cgattccgtt tgcactggaa agccctatca ccgccattcc
     4861 gtcctatatg gtcggcgcga ttgtcggctc aaccgccgct gtctggctgg gcgcagtgca
     4921 atggttcccg gaatccgcta tctgggcatg gccgctggtc actaacctcg gcgtctacat
     4981 ggcggggatc gcgctggggg cagtcattac tgcgctgatg gtggtgttcc tgcgtctgat
     5041 gatgttccgt aaaggcaaat tgttaatcga tagcctgtaa gaaggacaat cctgatgaca
     5101 ttactcgctt cgctgcgcga ctggcttaag gcgcaacaac tggatgcggt gcttctctcc
     5161 tcacggcaga acaaacagcc gcatctgggg atctccaccg gatcaggcta tgtggtgatt
     5221 agccgtgtaa gtgcgcacat tctggtggat tcgcgctact acgcggatgt agaagcccgc
     5281 acgcaaggct accagctgca tttgcttgac gcgacgaaca cgcttaccac tatcgtcaat
     5341 caaatcattg ccgatgagca gttgcaaacg ctcggttttg aaggccagca ggtgagttgg
     5401 gaaaccgcgc accgctggca gtctgaactc aatgcgaaac tggttagcgc cacgccggat
     5461 gtgctgcggc aaatcaaaac gccagaggag gtggagaaaa tccgcctcgc ctgtgggatt
     5521 gccgatcgtg gtgcggagca tattcgccgc tttattcagg cggggatgag cgagcgcgag
     5581 atagccgctg aactggagtg gtttatgcgc cagcagggcg cagaaaaagc ctcttttgac
     5641 accattgtcg ccagcggctg gcgtggggcg ctgccgcacg gtaaagccag cgacaagatt
     5701 gttgcagcgg gcgagtttgt cactcttgat tttggtgcgc tctatcaggg gtactgctct
     5761 gatatgacgc gcaccttgct ggtgaacggc gaaggggtga gcgccgaatc tcacccgctg
     5821 tttaacgtct atcaaattgt cctgcaggca cagctcgcag caatttccgc gattcgcccc
     5881 ggcgtgcgct gccagcaggt tgacgatgcc gcgcgccggg tcattacaga agcaggttat
     5941 ggcgactatt tcggtcataa caccggtcat gctatcggca ttgaagtcca tgaagagccg
     6001 cgtttttcac cgcgggatac cacgacgcta cagccaggca tgttactgac cgtggagccg
     6061 gggatttatt tgccagggca agggggcgtg cgcatcgaag atgttgtgct ggtcaccccg
     6121 caaggcgcag aagtgctcta cgccatgccg aaaacagtgt tgctcacggg agaggcataa
     6181 tggatttatc gttattaaaa gcgttgagcg aggcagatgc gatcgcctcc tcggaacagg
     6241 aagtacggca gatcctgctg gaagaagcgg atcgcctgca aaaagaagtg cgatttgatg
     6301 ggctgggatc ggtgctgatc cgcctcaatg aatcgacagg tccgaaggtg atgatctgtg
     6361 cgcatatgga cgaagtggga tttatggtgc gcagcatctc ccgcgaaggg gcgatcgatg
     6421 tgttgccggt tggcaatgta cgcatggctg cccgccagct tcagtcggtg cgtatcacta
     6481 cccgtgaaga gtgcaaaatt ccaggcctgc ttgacggcga ccggcagggg aatgatgtca
     6541 gcgccatgcg cgtggacatt ggtgcgcgct cgtatgacga agtgatgcag gcgggaattc
     6601 gtccaggcga tcgcgtcacc tttgatacca cttttcaggt tctccctcac cagcgggtga
     6661 tggggaaagc ctttgatgat cgcctcggtt gctatctgct ggtgacgtta ctacgtgagc
     6721 tgcacgacgc cgaactgcct gcggaggtgt ggctggtggc aagttccagc gaagaggtgg
     6781 gattacgcgg cgggcaaact gccacccgcg cggtgtcgcc ggacgtcgcc attgtgcttg
     6841 ataccgcctg ctgggcgaaa aactttgatt atggcgcggc taaccatcgc cagattggta
     6901 acgggccgat gctggtatta agcgacaagt cgctgattgc gccgccaaaa cttaccgcgt
     6961 ggattgaaac cgtggcggca gaaattggcg taccgttgca ggcggatatg ttcagcaacg
     7021 gcggcacaga tggcggagcg gtgcatttaa ctggcaccgg catacccacg gtggtgatgg
     7081 ggccagcgac gcgccacgga cattgtgcgg catcgattgc cgattgccgc gacattttgc
     7141 agatgcagca gcttttatct gcccttattc aacgtcttac ccgtgagacg gttgttcaac
     7201 tgacggattt cagatgatct cctgattaac ccggagcggt tatgttaacg attcaatttc
     7261 tctgtccttt gcctaacggt attcatgctc gtccggcgtg ggaacttaaa gaacagtgca
     7321 gccagtggca aagcgaaatc acttttatta accatcgcca gaacgcaaag gccgatgcga
     7381 aaagttcgct ggcgctgatt ggcaccggca ctttatttaa tgacagttgc agcctgaaca
     7441 ttagcggcag cgatgaagag caggcgcggc gcgtgctgga agagtacatc caggtgcgct
     7501 ttatcgacag cgacagcatc cagccaacac aggcagaact gacggcgcat ccgctgccgc
     7561 gttcattaag ccgtctgaat cctgatttac tgtacggcaa tgtgctggca agcggcgtcg
     7621 gcgtgggtac gctgaccctg ttacagagcg acagcctcga cagttatcgg gccatcccgg
     7681 ccagtgcgca agactccacc cggctggagc acagcctggc aacgcttgcc gagcaactga
     7741 accagcagct gcgtgagcgt gacggcgaaa gcaaaactat cctcagcgcc catttgtcgc
     7801 tgattcagga tgatgaattt gcaggcaata tccgtcgcct tatggcagaa cagcatcagg
     7861 ggctgggggc ggcgatcatc agcaatatgg agcaggtttg cgccaaacta tctgcttctg
     7921 ccagcgatta tctgcgcgag cgggtcagcg acattcgcga tatcagcgaa cagttgctgc
     7981 atatcacctg gccggaactg aagccgcgca acaatctggt gctggaaaaa ccgactattc
     8041 tggtggccga ggatttaacc ccaagccagt ttttgagcct cgatttaaaa aatcttgcgg
     8101 gcatgattct ggagaaaacc gggcgcacct cgcataccct gattctggcc cgtgcctcgg
     8161 cgatcccggt tcttagcggc ctgccgctgg atgcgatcgc gcgctatgcc gggcaaccgg
     8221 cggtgcttga cgcccagtgc ggtgtgctgg cgattaaccc gaatgacgcg gtgagcggtt
     8281 attatcaggt tgcgcagacg ctggcagata aacgtcagca gcagcaggcg caggctgccg
     8341 cgcagctggc ctattcccgt gataacaagc gcattgatat cgcggcgaat attggcaccg
     8401 ctctggaagc gcctggcgcg tttgccaacg gcgcggaagg cgtcgggctg ttccgtaccg
     8461 aaatgctcta tatggatcgc gacagcgcgc cggacgagca ggagcaattt gaagcctacc
     8521 agcaggtgct actggcggcg ggcgacaagc cgattatctt ccgcacaatg gacatcggag
     8581 gcgataaaag cattccttat ctgaatattc cccaggaaga gaacccgttc ctcggctatc
     8641 gcgcggtacg tatttacccg gaatttgcgg gcctgttccg cactcaactg cgggccattt
     8701 tgcgcgccgc cagtttcggc aacgcccagt tgatgatccc aatggttcac agcctcgatc
     8761 agatcttatg ggtgaagggc gagatccaaa aagcgatcgt tgagcttaag cgcgatggcc
     8821 tgcgtcatgc agagacgatt acgctgggta tcatggtgga agtgccgtcg gtgtgctaca
     8881 tcatcgatca cttctgcgat gaggtagatt tcttcagtat cggctccaac gatatgaccc
     8941 agtatctgta tgcggtcgat cgtaataacc cgcgcgtatc gccgctgtat aacccgatta
     9001 cgccatcgtt cctgcgcatg ttgcagcaga ttattaccac tgcgcatcag cggggtaaat
     9061 gggtaggcat ttgcggtgaa ctgggcggtg aaagccgtta tctgccgcta ctgcttgggc
     9121 tgggcctgga cgagttaagt atgagtagcc cgcgcattcc ggcggtgaaa agccagcttc
     9181 gtcaactgga tagcgaggcg tgtcgggaac tggcgcgtca ggcatgtgaa tgccgcagcg
     9241 cgcaggaaat tgaagcgtta ctcaccgcct ttacgccgga agaagacgtt cgcccactgc
     9301 tggcgctgga gaatatcttt gttgatcagt cctttagcaa taaagagcag gcgatccagt
     9361 tcctgtgcgg taacctcggc gttaacgggc gcactgaaca tccgttcgag ctggaagagg
     9421 atgtctggca gcgggaagag attgttacca caggcgttgg ttttggcgta gcgatcccgc
     9481 acaccaaatc tcagtggatc cgtcattcca gtatcagcat tgcccggctg gtgaaaccgg
     9541 ttgactggca gtcagaaatg ggcgaagtcg aactggtgat catgctgaca ctgggcgcta
     9601 acgaagggat gaatcatgtg aaagtcttct cgcagctggc gcgtaaactg gtgaataaaa
     9661 acttccgcca gtcgctgttc gccgcgcaag atgcgcaaag catcctgacg ctgctggaaa
     9721 cggaattaac cttctgaagt tagccctgaa agcgggcgct gtactctccc ggcgtcagac
     9781 caaactgacg ccggaagacg cgacaaaaat aatcgctatc cggaaaaccg caacgctgcg
     9841 ccacttcatg aatcgacaga tgatatttct gcaaaatcat cctcgccttc gccattcgca
     9901 cccagcgaac atactcgata aaacgcatcg tcccatgttg ggcaaacagt tttgacagat
     9961 gattgggcgt gatattaaaa aactgcgcca cgctctcgcg ggtgagcggc tgggcataat
    10021 tatcctgaac ccagttacaa atgctgtgat agagaaaatc gctgcgcggc tgcgaattgc
    10081 ccggtggcat attcaccacg ttgcggcaaa gatgcagcaa acttaacacc agtggctgaa
    10141 tgatattctg ctccagcggt gaacggctaa gatgggtgag ggcggtaagc atcgcttcac
    10201 cttcaccgcg ttgcaggctg ggaagctgga tctggcgagc aggatgcaac aacgacgtgg
    10261 tacggctatc gtaaaacgat aacccaagcc aggtcggtgc gaacaccagg cttaacagca
    10321 tcaccggttt gttgttgacc ggtaaattag ccgcgcgtgc cggaataaac agcatttctc
    10381 cttccaccat acgttgctgc tttgcttcca gcagattgcc gtattcgccg cgtagcacga
    10441 tatccagacg gggaaaatca atacataaac tgcccactgg caatgaggca ggctggctgg
    10501 caaaccagac gcgccccagt agttgcgggt taagcaccag gccgctgaac agatcggcga
    10561 aaaattgttg atcggcgggc aagcctggtg ctttcatgaa agttcccgat taaagatgca
    10621 ttaactggcg gaattcttta actttgctgc ggctgaccgg cacttcaaaa tccagatctt
    10681 tcagccgcag aatataggtg ttattaaacc acggttcgat ctcgcggatt ttgttcagat
    10741 tgacacaaaa tgagcgatgg cagcggaaaa aatgcgacgg cggcagcttg ctgcaaaact
    10801 cggtaatgtt catcggcatt acgtaggatt cacgccgcgt atagacaaag gtcattttct
    10861 cgtgcgcttc ggcgtaatag atatcgttaa ttggcgtgac aatgattcgc tcatctttca
    10921 ccagattaat ggtgtcattc tcacgcgtta ccgtcgcggc aggcgtactg ctggtctgct
    10981 gttgttgcca ggctgcttcc agtttttgca gcatcccggt aatgcgtgac tcctgatacg
    11041 gtttgagaat gtagtcaaac gcttccagtt caaatgcttc taccgcatgt tctttccacg
    11101 cggtgatgaa cacaataaac ggtttatggg cgaactggct gatgttttgc gccagcaaca
    11161 cgccatccag cgacggaata ttgatatcca gaaaaatggc gtcgacgcgg ttatgctgca
    11221 aaaacttcaa cacgtccaga ccgtcgtcaa aggtgccgac aatctccatc tggctgtgct
    11281 ctttaattag ccagctcagt tcctgttgtg ccaggaattc gtcttcaaca atgatgactt
    11341 tcacaatatc actccggctc aaagcaataa cgtagcctgt gaggcgactg gggtgcgttg
    11401 gttaggaatg taaaacgcaa tttccgttcc tggttccagg cggcggatat gcagcccctc
    11461 gccatacaat aacttcacgc gatgatggac attcagcaaa ccaattttat tgcccggcat
    11521 ttcgttcgct tcgacccgct caatgacttt tggatcgatg ccgtgcccgg tgtctcgcac
    11581 cgcaatgcgt acccgattac cgcactctgc aacgctgatg gtgacaacgc ctttaccttt
    11641 gcaaggctga ataccgtgga caatggcatt ctccaccaac ggctggatca gcaggctggg
    11701 aatgcagcaa ttcacctctt catcaatatc atagataacc gtcagcttgt cgccaaagcg
    11761 ggcctgctca atggcaatat agtctttaat ttgatacagc tcttttttga tatcgatttg
    11821 ctcatcgtct tttaattcaa tgttatagcg cagataacgc gacagattaa agatcagctg
    11881 gcgagcggta tccggattca gacggatcga cgatgaaata gcgttcaaag cgttaaacag
    11941 gaaatgggga ttaattttgc tttgcagggc gcgaagctct gccttatttg ccatttcacg
    12001 tagctgctcg gcgcgtgaaa cctccagttg cgtggagatg atttgcgaca gaccgaccgc
    12061 catttcctgt aatgatgacg taatctgatg cgcgtggcag tagtaaattt tcagtgttcc
    12121 ggtcacaacg cctttctccc acaatgggat caccagcatg gaatgaatct ctggtgtgcg
    12181 gtgggcttca tcattgtttt taatgatgat ttttccgtaa ttcatcgcct gtcgggtagt
    12241 cgggctaatg aagtcatcgc cattctgata gttgtgttca cccacgccaa cataggccag
    12301 cacatgatcg gtattggtaa tcgccaccgc atcggcatga atatcatcgc ggataatttc
    12361 acaaactttg cgtaatgact cgctattgac atggcgaaac agcggcagcg ttttgttggc
    12421 gatatccagc gccagcttgg cctgccgcgc ggcgctggcc tctttttcgc cctcaacgct
    12481 ttgcaccagc agcacaataa agccgataca gacgctaccg aggatcattg gaatcccgat
    12541 tttagagacg atatcgatcc ccaacgcggt agttggtgcc cagacaatca ccagaatcat
    12601 ggtcaacgtt tcacataaca tgccgccgag aataccgacg cgccagcgct gtgctttggg
    12661 gattttcagg ttgatccagc ccgatataca acccgccaga atgctggtga taaagcaggg
    12721 gatcgccgtc acgccgccga tatcaattaa ataccggtga atacccgcaa tcacgccggt
    12781 aatgatgcct acccacgggc caaacagaat cccgcctgac attaccgcga taatacgaac
    12841 gttcaccagc gagccttcta cgggaacgcc ggaccaggtg ctgaacaggg caaacaacga
    12901 gaaaatggcg gtaacggcga gcagctcttt cggtgagtgt gccgacttgt gcaacagttc
    12961 gcgaaacagg cggatacgga tgagaaagaa caggcagata agcattaacg ctgcccgatc
    13021 gaagaccgcc agcagcatgt tgaatatttc gtgcacgggt gactctgaat aaggattaaa
    13081 cctctatgat aggtaacctg aaggctgatg accagcaggc cgtttttgag gaaaaaccac
    13141 aattttaaaa ggggattgaa agataaatgg tgtattagca atgcatctct tgataatgca
    13201 catctgcttg gtaaatttat tgaaattata atgttaataa aatgagtggt aattttcttg
    13261 ttagcgcaga taaatttcct gtttttgaag tggcgttcct catttaaagc cattgataaa
    13321 aatgttctaa aacgctggac agacagaaat taatcaggct attttcaaaa cagccaccat
    13381 cgtggcagcg tcgctcggac ggtccgggcg ctaacgttaa tctgaggaaa ttatggctga
    13441 cactcgccct gaacgtcgct ttacgcgcat tgatcgtctc ccgccctacg tttttaatat
    13501 taccgctgaa ctgaaaatgg ctgcgcgtcg gcgcggcgaa gatattatcg atttcagtat
    13561 gggtaacccg gacggtgcga ctccgccgca catcgtcgaa aaattatgta cggtggccca
    13621 gcgcccggac acgcatggtt actccacttc acgcggcatt ccgcggctac gtcgcgccat
    13681 ttcacactgg tatcaggatc gctacgacgt cgagatcgac ccggaatcag aagccatcgt
    13741 caccattggt tcgaaagagg gactggcgca tctgatgctg gcgacgctgg atcatggtga
    13801 cactgtactg gtgcctaatc caagctaccc gattcatatt tacggcgcgg tgattgccgg
    13861 ggcgcaggta cgctcagtgc cgctggtgga aggtgtcgat ttcttcaacg aactggaacg
    13921 cgccattcgc gaaagttatc cgaaaccgaa gatgatgatc ctcggcttcc cgtcgaaccc
    13981 aaccgcgcaa tgcgtggaac tggaattctt tgtgaaggtt gtggcgctgg cgaaacgcta
    14041 cgatgtgctg gtggtccatg acctggccta tgccgatatc gtctacgatg gctggaaagc
    14101 gccgtcaatc atgcaggtgc cgggtgcacg cgatgtggcg gtcgagttct ttacgctgtc
    14161 gaaaagctac aacatggcgg gctggcgtat cggctttatg gttggcaaca aaacgctggt
    14221 cagcgcgctg gcacgtatta aaagctatca cgattacggc acctttacgc cgttgcaggt
    14281 ggcagcgatt gcggcgctgg agggcgatca acagtgcgtg cgcgatattg ccgaacagta
    14341 caaacgccgc cgtgatgtac tggttaaagg gctgcatgaa gcgggctgga tggtcgaaat
    14401 gccgaaggct tcgatgtatg tctgggcgaa aatcccggaa ccgtatgctg ccatgggctc
    14461 gctggaattt gccaagaagc tgctgaacga agcgaaggtc tgtgtctctc cggggattgg
    14521 ctttggcgac tacggcgaca cgcatgttcg ctttgcactg attgaaaacc gcgaccgtat
    14581 tcgtcaggcg attcgtggaa ttaaagcgat gttccgtgcc gacggtttac ttcccgccag
    14641 cagcaaacat attcacgaaa acgcggaata aacaccatcg ccagaaatgc aaacaggagc
    14701 cagatggctc ctgtttttca ttgcacagtt tgcttttaga tcatcagggc aaaagtaccg
    14761 gcccaaacga tgaccataaa agaaatgccc ataaagaaat atttcacttt acatctctcc
    14821 gcgtaattac tgttacgcct gaatgacttc attacacctg atacagtgag ttgacaccgg
    14881 gcaaaacgac cttaaaagtc ctgtatttta ccgtgggcat caccccagaa ttctgagcta
    14941 aactgctcca gacggcgcta atgtacgcct aaaactgaaa cgagacaaga ggcattttct
    15001 tatttacttt tagtaactta caggtgtctc atatcggcga cagtttaatt tctgacggta
    15061 ataattagag attcagcgaa ggaaaaaacc ttaagaaaaa agccataaaa accatgaggt
    15121 tattatggct gatttgagga gggaaagagt tagagcagtt tgttaaatgt atagcgacga
    15181 ttctcccacc gggcgcgttt taaagcgacg gtggatccag aggtactgct ccggtgcgcg
    15241 catgatctct ttttcgataa tcttgttcat ataggcagcg gcttgatttt catctgccgg
    15301 gtagccttcc atctctgggg tgatgtacaa acgatatccg ctgtaatccg cttttcttac
    15361 catcgttacg gttaacatgg ctgcgccaga gagacgtgaa aggacatacg taccattggt
    15421 tgtggcgaca ttttccaccg caaagaacgg cgcgaaggag ctgcctttac gaccataatc
    15481 ctgatcggga gcaaaccata ccgcttcacc tttcttcagt gcaccgacaa tgccgcgcag
    15541 attatttctg ccgatcatcg ctttgttaga gcgcatgcgc ccacgggtct gcacccattc
    15601 catcagctga ttattatgtg gacgataggt agccatcatt ggttggcaca gtcccatcac
    15661 gcggccgccc agttccagcg acataaaatg gacgccgaca accattacgc cgcgattttg
    15721 catttgtgcg cgtttaaggt tatccaaccc ttcaacatca aaccatttac gtacgcgact
    15781 gtcgggccag aaccacgcca tgccggtttc taccagcgcc atgccgagtg aacgaaagtt
    15841 ttcggcaatc atcttctcgc gttcttccgc agaatgctgt gggaaacaaa gttccaggtt
    15901 tttacgggcg atagattcac gccgtttcag gaatggtcgc gctattgcgc caatacgcgt
    15961 gccgagaaag cagagaacag gataaggaag ctgtacccaa agccagagta caccaagccc
    16021 aaaccatgtg agccagtagc gaggatgtag aaactcgcgg gaaaatttgc attgtggaaa
    16081 catagttacc ttctgtagat acgaattgtt gtatcgcaga agattccatg tcgacagcgg
    16141 tacactcaat tggttgggcc atcatgtacg acaatggttc ctgtaaagag tgtaaagact
    16201 agtcaggaca taaccacgag gagcatgaat gcggtggctc tactttatca ctggctgact
    16261 gctttcagct actgattatt aatcgtgcaa agtaaaaagt tttccccacg caatagtgtt
    16321 gcatttaagg tgctgttttc attgatgctt agcaaaaatt gccagaggtt aaggattgtc
    16381 ttactattaa agtgactgaa gttacaggtt ttttagcgcg gcaggaggtt gagctatgat
    16441 ttatttatgg atgtttctcg ccctgtgtat tgtctgcgta agcggctaca ttggtcaggt
    16501 cctgaacgtg gtatcggcgg tctcttcttt cttcggcatg gtgatcctcg ctgcactcat
    16561 ttattacttc accatgtggt taactggcgg taatgaactg gtgacgggga tatttatgtt
    16621 tcttgccccg gcttgtggct tgatgattcg ctttatggtg ggctatggca ggcggtagtg
    16681 ggtcaggatg gttcatttgg gcaggtgttc acatcagcat cagacatgat tgttcacctg
    16741 ccatgtgaca gacgctatcg cacaggatga ataagataat tcgcctttgt cgaaaaaggg
    16801 ggcgtaatgt cttctgtgag atttaatggt ggcgcatctt ctctcatctt aaacaaaatc
    16861 gcctgtgatg gctgaagttt tgtagagtgt gtggtcgtca tcgctgacgt tttttccgtc
    16921 tctttatagc tcgcctcact accaccacca gaaatagaaa atgcaaaaac attgcatgcc
    16981 ataatagaga ccaataaaaa taagctaaaa agcattaagt ttactttcat agaaagttat
    17041 cctgaaaatg gttcactgaa aacctacg
//
LOCUS       DAYPHN010000106        16920 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000106 DAYPHN010000000
VERSION     DAYPHN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.106"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..562
                     /locus_tag="ACG0BW_003232"
     CDS             <1..562
                     /locus_tag="ACG0BW_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFK7260158.1"
                     /translation="DEDNKFDNFMRQNRFSKMNFDTQQMIDFINRFNMNKWLIDQNNN
                     FFIQLIDQALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEVEDNLRTFNTNELTRNDN
                     IFILFSSVYDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGS
                     KSQDKHALFPIFTASWRANRIKNKGI"
     gene            785..1282
                     /gene="ubiC"
                     /locus_tag="ACG0BW_003233"
     CDS             785..1282
                     /gene="ubiC"
                     /locus_tag="ACG0BW_003233"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFK7260159.1"
                     /translation="MSHPALTQLRALRYFKEIPALDTQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1295..2167
                     /gene="ubiA"
                     /locus_tag="ACG0BW_003234"
     CDS             1295..2167
                     /gene="ubiA"
                     /locus_tag="ACG0BW_003234"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFK7260160.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2322..4745)
                     /gene="plsB"
                     /locus_tag="ACG0BW_003235"
     CDS             complement(2322..4745)
                     /gene="plsB"
                     /locus_tag="ACG0BW_003235"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFK7260161.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4916..5284
                     /gene="dgkA"
                     /locus_tag="ACG0BW_003236"
     CDS             4916..5284
                     /gene="dgkA"
                     /locus_tag="ACG0BW_003236"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFK7260162.1"
                     /translation="MANNTTGLTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5394..6002
                     /gene="lexA"
                     /locus_tag="ACG0BW_003237"
     CDS             5394..6002
                     /gene="lexA"
                     /locus_tag="ACG0BW_003237"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFK7260163.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6075..7400
                     /gene="dinF"
                     /locus_tag="ACG0BW_003238"
     CDS             6075..7400
                     /gene="dinF"
                     /locus_tag="ACG0BW_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFK7260164.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7516..7725
                     /gene="yjbJ"
                     /locus_tag="ACG0BW_003239"
     CDS             7516..7725
                     /gene="yjbJ"
                     /locus_tag="ACG0BW_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFK7260165.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7767..8282)
                     /gene="zur"
                     /locus_tag="ACG0BW_003240"
     CDS             complement(7767..8282)
                     /gene="zur"
                     /locus_tag="ACG0BW_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFK7260166.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
     gene            8600..9562
                     /locus_tag="ACG0BW_003241"
     CDS             8600..9562
                     /locus_tag="ACG0BW_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFK7260167.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSAM
                     RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDRE
                     FIASVVDRAIFHFAINSICNPEDNKDATPIERCTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEYDDNSLKLFASLLISLSYSSAYVDLAGAERVKINE
                     YNENYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDDYLDKMSLLVNEGKLEPDILS
                     KMRDAAPKLIDFAKSFDPNSKEKIKILTDTSKLIYDLFGVKSEK"
     gene            9925..10962
                     /gene="dusA"
                     /locus_tag="ACG0BW_003242"
     CDS             9925..10962
                     /gene="dusA"
                     /locus_tag="ACG0BW_003242"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFK7260168.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            11096..11338
                     /gene="pspG"
                     /locus_tag="ACG0BW_003243"
     CDS             11096..11338
                     /gene="pspG"
                     /locus_tag="ACG0BW_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFK7260169.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAVLVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11504..12487)
                     /gene="qorA"
                     /locus_tag="ACG0BW_003244"
     CDS             complement(11504..12487)
                     /gene="qorA"
                     /locus_tag="ACG0BW_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFK7260170.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVSVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12570..13985
                     /gene="dnaB"
                     /locus_tag="ACG0BW_003245"
     CDS             12570..13985
                     /gene="dnaB"
                     /locus_tag="ACG0BW_003245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFK7260171.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRTLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            14038..15117
                     /gene="alr"
                     /locus_tag="ACG0BW_003246"
     CDS             14038..15117
                     /gene="alr"
                     /locus_tag="ACG0BW_003246"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFK7260172.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCDATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15370..16563
                     /gene="tyrB"
                     /locus_tag="ACG0BW_003247"
     CDS             15370..16563
                     /gene="tyrB"
                     /locus_tag="ACG0BW_003247"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFK7260173.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNCVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         4086 a         3988 c         4434 g         4412 t
ORIGIN      
        1 tgatgaagat aacaagttcg ataattttat gcgtcaaaat cgattcagta agatgaattt
       61 cgatacccaa cagatgatcg attttattaa cagatttaat atgaataaat ggctgattga
      121 tcaaaataac aattttttta tccaacttat cgatcaggct ctacgatcaa cgaatgatac
      181 gatcaaagaa aatgcctggc atctttataa agagtggatt cgtagtgatg atgtttcacc
      241 tttatttata gaagttgaag ataatttaag aacctttaac acgaatgaat taacacgaaa
      301 cgataatatc tttatcctgt tctcctctgt ctatgatggg ccagttatgg tggtaagctc
      361 ccagcgctta catgatatgt tgaatcctac aaaagatacc aattggaatt ccacgtatat
      421 ctacaaatcc agacatgaga tgttgcctgt taatcttact ccggaaacac ttttcggctc
      481 taaatctcag gataaacatg cgcttttccc aatttttact gcgagttggc gagctaatcg
      541 tataaagaat aaaggtattt aagtaaagga aaacatcatc gttcctggca tcctgggcgg
      601 tgatgtccct atggttgccc tcgccagcac gggcatcggc aaagcgtaag gttcaacagc
      661 gttttaccac ttcatgcgat tgttgcgttt ttgttgcgca ttagatcact taatttgctt
      721 tacatctccc gtaaacactt ttctgcgata caatgccttt acgttatgta acggagagtt
      781 cggcatgtca caccccgcgt taacgcaact gcgtgcgctg cgctatttta aagagatccc
      841 tgccctggat acgcaactgc tcgactggct gttgctggaa gattccatga caaaacgttt
      901 tgaacagcag ggaaaaacgg tgagcgtgac gatgatccgc gaagggtttg tcgagcagaa
      961 tgaaatcccc gaagaactgc cgctgctgcc gaaagagtct cgttactggt tacgtgaaat
     1021 tttgttatgt gccgatggtg aaccgtggct tgccggtcgt accgtcgttc ctgtgtcaac
     1081 gttaagcggg ccggagctgg cgttacaaaa attgggtaaa acgccgttag gacgctatct
     1141 gttcacatca tcgacattaa cccgggactt tattgagata ggccgtgatg ccggactgtg
     1201 ggggcgacgt tcccgcctgc gattaagcgg taaaccgctg ttgctaacag aactgttttt
     1261 accggcgtca ccgttgtact aagaggaaaa aaatatggag tggagtctga cgcagaataa
     1321 gctgctggcg tttcatcgct taatgcgtac ggataagcca attggcgcgt tactgctgct
     1381 ctggccaaca ttatgggcgt tgtgggtggc gacaccgggc gttccccagc tctggatcct
     1441 ggcggtgttt gtcgcgggtg tctggctgat gcgcgctgct ggttgcgtgg tgaatgatta
     1501 tgctgaccgc aagtttgatg gtcatgttaa gcgcacggcg aaccgaccgc ttcccagcgg
     1561 cgcggtaacg gagaaagagg cgcgcgcgct gtttgtcgtg ctggtactga tttcgttttt
     1621 actggtgctg acgctgaata cgatgaccat tctgttgtcg attgccgcgc tagcgctggc
     1681 gtgggtgtac ccgtttatga agcggtatac ccatctaccg caagtggtgc tgggcgcggc
     1741 gtttggctgg tcgattccaa tggcttttgc tgctgtgagt gagtcggtgc cattgagttg
     1801 ctggttaatg ttcctcgcca atattctctg ggcggtggca tacgacacgc agtatgcgat
     1861 ggttgaccgc gatgatgatg tgaagattgg cattaaatcc accgcaatcc tgttcggcca
     1921 atacgataaa ttgattattg gtatattgca gattggcgta ctggcgttga tggcgattat
     1981 cggtgagtta aatggcttag gctggggata ttactggtca attctggtgg ctggcgcgct
     2041 gtttgtttat caacaaaaac tgattgccaa ccgcgagcgt gaagcctgct ttaaagcgtt
     2101 tatgaataat aactatgttg gtctggtact gtttttaggg ctggcaatga gttactggca
     2161 tttctgatga tgtaaaaaag ccggatgatc atccggcttt cttctgggct gcctgatgcg
     2221 ctgcgcttct cagacctaca caacatatcg caatttattg aatttgcaga tcatggtagg
     2281 ccgaataagg cgtttgcgcc gcatccggca attctcactg attacccttc gccctgcgtc
     2341 gcactctcaa tcgtcaaacg cacgtctgat gtaatcaact ccgccagcaa ctgatacacc
     2401 ttcatcgttt ctgccggttc ggcgtcgccg ctatcgctga tatacccttc atcacgcagt
     2461 gtcagcacca gagaactgaa caccgccttg tcgaagaact ccggcgcgtt gataccgtgc
     2521 agcacggaga gacgttgcgc gacggtgcgg ctctctttct ccagcgtacc gcggttgatc
     2581 gacgggttgg cgctcaacag ccaaaaagtg atggcataac gttgcagcgt ttcgcgcgcg
     2641 cctgcggcca gcagctgtag cgtgcgagaa tgcgccgggt tgatatgcaa ctcatcatct
     2701 tgcagggtaa tcagcccctg acgttgcatc tcatttgcca gcgcatcaat aacgtccggt
     2761 aactcgtcgc gatcccagcg caggaacagt tccgctttca gcattgggta aagcacattg
     2821 acgtgctcca tcaatacgtc gcgggagatg tggcgatgct gggtgacaat tgccgccatc
     2881 agcgaaggca gcaccaacat atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct
     2941 tgctcgcgcg gcagaatgat gatgtcgccg atagtgtctt tctcaacttc aaacttgttc
     3001 atttgcagcg cgtgatcgat aagctcgctg gcgctggctg aaggaacggt agagtccgtg
     3061 gagtaaggta cgttgcgcat cagatccagg tagcagttga gttgctcggt taactgctcg
     3121 cgggtgagtg agcgctgacg tgacgccagt agcgcagtac agcacaggtt catggcgttt
     3181 gccgcgcctg cgttgttaat gcgtaccatc agatcggcag caatattatt gaccgtcggc
     3241 gttaaccagg ccggacgcac cgcttcgatg ggatcgatag attcacgcca gtctggtacg
     3301 tgctggttaa ggtaggtcat caacggcatt ggttcaccga agttgacgta accctgaccg
     3361 agattacgca gcttgcttaa accgcgcagc atctgcggca ggctctcttt ctctttcgtc
     3421 gcgccgcgca gttctttggc gtaagtaccc acttccatga cgtgctcata accgatatag
     3481 atcggaatca gcgtaatcgg acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac
     3541 agcgtaccgg ttttcggatc cagcaaacgc cccgtacggg aacgaccgcc ttctacgaag
     3601 tactcgacgg aataaccacg gctgaacagt tcgccgagat actcacggaa aacggtggaa
     3661 tagagcttat tgcctttaaa cgtacggcga ataaagaacg cacccagacg gcggaaaatc
     3721 ggcccggccg gccagaagtt caggttgatc ccggcggcga tatgcggcgg caccagcccc
     3781 tggtgataca gcacgtaaga aagcagcagg tagtccatgt gactgcggtg gcaaggcaca
     3841 tacaccagct catgaccgtc gtgggccaac tggcgaacgc gctcggcgtt atggacgttg
     3901 ataccctggt aaagtcggtt ccaggtgaag cccagaatac ggtcagtcag gcgaatcatc
     3961 tcgtaagaga aattcgccgc aatctcttcc atcagtgcaa tcgcgttctg ctgcgctttt
     4021 tcatgggaga tttttttgct gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag
     4081 gcgagcagct tattaaacag atcctgacga gcaggcaaac gtgggcctac ggcagccaga
     4141 cgttgacggg caaagtgcat acgcgccacg cgcgccagtt tttgcgcgat agttttatcc
     4201 gtgccgtgtt catccgccat acggcgcagc gaaactgacg gtgagaaacg cacaaaactg
     4261 tcgcgaccga gccacagtac ggcgaaaaat ttctgtacgc cgttaagcat acgcagtggc
     4321 gggtttactt cgcctttttc acgccccggc gcgcgaccaa acatcaccga tactggcacc
     4381 atctgcacat ccagatttgg gttgctacgg tgcaaatcga gatagtcgtg gaacagctta
     4441 atagactctt ctttcggcgt gtaataggtg aatacacgcg gcccgccgtg aatgaacaca
     4501 tagcgcggca gtagcgtgcc gtcgatttcc agcggctcta acgggtcagg caagtcatga
     4561 gccagacact gggcgcgcaa cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac
     4621 ataattggac gagaagtatc cagccccagt tccggggcag gatctgccgg aatagacttg
     4681 ctttttacca ggatgcttaa tggtaaattc agtaatttgt agtaaattcg tggccagccg
     4741 gacataaacg atgtaaagcc tctggttaat aatgcaaatg cgcggcaagg atagcagaaa
     4801 gtcatgggaa attctgtggt atccgctcat gtttcgcgcg gcgttacgca aacccgaatc
     4861 atcggattta acggtacact gatattgacg ctcataatgt aaaaaggttc tttcaatggc
     4921 caataatacc actggactca cccgaattat caaagctgct ggctattcct ggaaaggttt
     4981 acgcgctgca tggatcaacg aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc
     5041 ggtggtcatc gcctgctggc tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc
     5101 cgtgatgctg gtgatgattg tggaaatcct caatagcgcc atcgaagcag tggttgaccg
     5161 aattggctct gaataccatg agctatccgg acgcgctaaa gatatgggat ccgccgcggt
     5221 gctgattgcg attatcgtcg ccgtgattac ctggtgcatc ctgttatggt cgcattttgg
     5281 ataacccttc cagatttcga taaatctctg gtttattgtg cagtttatgg ttccaaaatc
     5341 gccttttgct gtatatactc acagcataac tgtatataca cccagggggc ggaatgaaag
     5401 cgttaacggc caggcaacaa gaggtgtttg atctcatccg tgatcacatc agccagacag
     5461 gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg
     5521 ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat tgaaattgtt tccggcgcat
     5581 cacgcgggat tcgtctgttg caggaagagg aagaagggtt gccgctggta ggtcgtgtgg
     5641 ctgccggtga accgcttctg gcgcaacagc atattgaagg tcattatcag gtcgatcctt
     5701 ccttattcaa gccgaatgct gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata
     5761 tcggcattat ggatggcgac ttgctggcag tgcataaaac tcaggatgta cgtaacggtc
     5821 aggtcgttgt cgcacgtatt gatgacgagg ttactgttaa gcgcctgaaa aaacagggca
     5881 ataaagtcga actgttgcca gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc
     5941 agcagagctt caccattgaa gggctggcgg ttggagttat tcgcaacggc gactggctgt
     6001 aacatatctc tgagaccgcg atgccgcctg gcgtcgcggt ttgtttttca tctctcttca
     6061 tcaggcttgt ctgcatggca ttcctcactt catctgataa agcactctgg catctcgcct
     6121 tacccatgat tttctccaat atcaccgttc cgttgctggg actggtcgat acggcggtaa
     6181 ttggtcatct tgatagcccg gtttatttgg gcggcgtggc ggttggtgca acggcgacca
     6241 gctttctctt tatgctgttg ctttttttgc gcatgagcac taccgggctg actgcgcagg
     6301 cttatggtgc caaaaatcct caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg
     6361 cgttgggggc cggggcgtta attgcgctgc tgcgtacgcc gattatcgat ctggcgctgc
     6421 atattgttgg cggcagcgaa gcggtgctag aacaggcgcg gcgctttctt gaaatccgct
     6481 ggttaagcgc accggcgtcg ctggcgaatc tggtattact tggttggtta ctcggcgtgc
     6541 aatatgcccg tgcaccagta attttgttag tggtcggcaa tatcctcaac attgtgctgg
     6601 atgtctggct ggtgatgggg ctgcatatga acgtgcaggg cgcggcgctg gcgacggtta
     6661 ttgcggaata tgcaacattg ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac
     6721 gcggaatctc cggcgaaatg ctgaaaactg cctggcgagg aaacttccgt cgcttgctgg
     6781 cgcttaaccg cgatatcatg ctgcgctcgc tgttgttgca actctgtttc ggcgcgatca
     6841 ccgtacttgg cgcgcgactg gggagtgaca ttatcgctgt taacgcggtt ctgatgacgt
     6901 tactcacctt taccgcctat gcgctggatg gttttgccta cgcggttgaa gcacattctg
     6961 gtcaggcgta cggtgcgcgc gacggtagcc agttactgga tgtctggcgg gcagcgtgcc
     7021 gccagtcggg gattgtagcg ttactgtttt cggtggttta tttgctggca ggggaacaca
     7081 tcattgcatt gctgacgtcg ttaacccaga ttcagcagct ggccgaccgc tatcttatct
     7141 ggcaggtgat tttgccgttg gtcggcgtct ggtgttatct gctcgacggc atgtttatag
     7201 gtgcaacgcg cgccgccgaa atgcgtaaca gtatggcggt ggccgccgca ggttttgcgc
     7261 tgacgctcct tacgctgccg tggctgggta atcatggttt gtggctggca ttaaccgtct
     7321 ttctggcttt gcgcgggctt tctctggcgg ctatctggcg gcgtcactgg cgcaacggta
     7381 cctggtttgc cgcaacgtga cggttaaaaa ttctgaataa ataatcctaa gccaaattgc
     7441 tgactacact taatctcacg ttcagaagaa aagtgaacgt actctcattc acaacctaac
     7501 gatgaggtct tgattatgaa taaagatgaa gccggcggta actggaaaca gtttaaaggt
     7561 aaagtgaaag agcaatgggg caaactgacc gatgatgata tgacgatcat tgaaggtaaa
     7621 cgtgatcaac tggtcggtaa aatccaggaa cgttatggtt atcagaaaga tcaggcagaa
     7681 aaagaggtcg tggattggga aacccgcaat gagtatcgct ggtaattaat ccctcctgcc
     7741 cgacgtgtac aaggatgtac gccctcttaa cgtgatttct ttttcacctg tacagagtga
     7801 tcatgctggc actgttcagg atgacgacac gcttccactt ccacgcatgc cgcacagagt
     7861 ccatgtgctt caatcacatt atgccgcagg gcaaacccca ttttggccgc cagcgtatgc
     7921 ataatgtctt ccacgccttc tgcacactct tctttcactg cgccgcagcg atcgcaaata
     7981 aacatggctg aagtatgagt gggctggtcg aacagatgac agagcacata actgttggtg
     8041 gattccacct tatgcacaaa accttgctca agcagaaaat ccagcgcgcg ataaaccgtt
     8101 ggcggcttgg cttgaggttc agcttcgcgc agcaaatcaa gcagatcata agcgctgata
     8161 gcgccatcct gcagactcat caggcgcaac acttccaggc gctgtggggt caggcgcaca
     8221 ttacgctgcg cacagatttt ttcagcctgc gctaataact cctgcgttgt ggtcttttcc
     8281 atcgggcacc tcaaagtggg ggttaagaat gtccttactt taccatgttc caggaaaaac
     8341 accgagaacc cttaatgttg ccgtaatgtt gattttttga tttgtcggta aggtgttgtg
     8401 ttgcctggta gtaccattat ctacacgata ataattaata attcattttt aaataaattg
     8461 cttcaagata actcgctatt tatttagctg ggtttgatcg catacctgta cttcagaaat
     8521 tatgagcgat ttttgatagt gagcagatgg caaaagaatt ataaggagga cacggtacga
     8581 caaaaaaggt agatgaatca tgttgaagat aatacccggt gcaactggat actttaataa
     8641 aacactcaat tcaaatcaat tcgataatga agatgcaatc aaaggtaaag taaatagtat
     8701 atatggtaaa tctattgatt attcagcgat gcgtcatcgg gatataatta ttgcaaaaat
     8761 agatttgttt attcaaagaa tcactcataa tttatggact gcacgtgaaa aaaatgtgac
     8821 gttaattaag caaataaatg atctcaaaat gtgtgtcaat gagtatattg gagattgtac
     8881 tgatgaggaa ttaaacgatc gtgaatttat tgcatcagtt gttgaccggg ctatttttca
     8941 tttcgcgatt aatagtatat gtaatcccga ggataataaa gatgcgacgc ccatagaacg
     9001 atgtactttt gacgtagaaa ctaagaatgg ccttccctcc acggttcagc ttttttatga
     9061 ggaatctaaa gataatgaac ctttagcgaa tatacatttg caagcaatag gttctggttt
     9121 tttaacgttt gttaatgcat gtcaggaata tgatgacaac agcttaaaat tattcgcttc
     9181 gctgttaatt tcactttcat attctagtgc ctacgtagat ttagcaggag cagaaagagt
     9241 gaagattaat gaatataatg agaactatct gacagctcag tttgaagaat tatctcaacg
     9301 tgatatgaag aagtacctgg gagagatgaa gcgtctggca gatgggggag aaatgaattt
     9361 tgatgactat ctggataaaa tgtcacttct ggtgaatgaa ggaaagctcg aacctgatat
     9421 tttaagcaaa atgcgagatg ctgcaccgaa attaattgac tttgctaagt cgtttgaccc
     9481 aaactcaaag gaaaagatta aaatacttac agacacttct aaattaattt atgatttatt
     9541 cggggttaaa tcggagaaat aatatgtgaa gttcttcgat agtatggaag gcattatata
     9601 aaaggaccca atgcttattg ggttcttttt ttctctatca gtgctattag cagggagaga
     9661 tatcaccgta gtttaatgtg tgatttttat ttatcgtcga acctggattg tttatcattg
     9721 gccttaacaa agctaacggc taataagatt atttccatca cttcgtgaga acttcatgcc
     9781 ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt tatggtatag
     9841 taccccgcta ttgagcctcc tgaacagtga tgctgaataa cataacccca tgatatatcg
     9901 ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa
     9961 accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag cgttgcacca
    10021 atgctcgact ggacggacag acattgccgc tatttcttgc gtctgctttc ccgcaatacg
    10081 ttgctgtata ccgaaatggt gactacaggg gcgattattc acggtaaagg tgattacctg
    10141 gcgtacagtg aagaagaaca tccggtagca ttgcaactgg gcggtagcga tccggcggcg
    10201 ctggcgcagt gtgcaaagct ggcagaagcg cgcggatatg atgagatcaa cctgaatgtc
    10261 ggctgcccgt ctgaccgggt gcagaacggc atgtttgggg cgtgtctgat gggtaatgcg
    10321 cagctggttg ccgactgcgt gaaagcgatg cgcgatgtgg tgtcgattcc ggtgacggtg
    10381 aaaacgcgta ttggcatcga cgaccaggac agctatgaat ttctctgcga tttcatcaac
    10441 accgtttccg gcaaaggcga gtgtgagatg ttcatcatcc acgcacgtaa agcctggctt
    10501 tcggggttaa gcccgaaaga aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat
    10561 caactgaagc gtgactttcc gcatctgaca atgtcgatta acggtggcat caagtcgctg
    10621 gaagaggcta aagcgcattt gcaacatatg gatggcgtga tggtcgggcg cgaagcgtat
    10681 cagaatccgg gtattctggc ggcggtagac cgagagatct ttggttcctc ggataccgat
    10741 gccgatccgg tggcggtagt gcgcgccatg tatccgtaca ttgagcgtga actcagccag
    10801 gggacgtatc ttggccatat tacccggcat atgctgggct tgttccaggg tattcctggc
    10861 gcgcggcagt ggcggcgtta tttaagtgaa aatgcccata aagcgggtgc agacattaat
    10921 gtgctggaac acgcgctcaa actggtggcg gataagcgtt aacttttcac caaaaaatag
    10981 tcaaattcac cacgccctgc gcaccgtcgc ggggcgtttt gctgttaaat caatagatta
    11041 tttttggcat gattcttgta atgccagcaa gagatttcat atttgggaga gcatcatgct
    11101 ggaactactt tttgtgattg gcttttttgt catgttgatg gtcaccggcg tttcgttgct
    11161 gggcattatc gccgtgctgg ttgtggcgac ggccattatg ttcctcggcg gtatgctggc
    11221 attgatgatt aagttgctgc cgtggttact cctggcgatt gcggtggtgt gggttattaa
    11281 ggcgattaaa gcaccaaaag tgccgaaata tcagcgttat gaccgctggc gttactaagg
    11341 gattgtgcgg atgatcacaa cctaaggttt tatccttaga acaaaatagg aattgataat
    11401 caaatctgtc actattgcgc ctctaacaga ttcatcgtgc tgtaccctac atacagccga
    11461 actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga atcaacaggc
    11521 tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca tccttcagcg
    11581 gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc aaagagaaca
    11641 gttcattact ggcctcggtt aattcctccc gcgtggtgat atagccttgc agggaagggc
    11701 gtgtcacata caacgagcct ttctgattga gaatgcctaa gttcacaccg gtaaccgcac
    11761 ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc agcgaccgct
    11821 cccaggtgtc tctgcccacg gaatcgtaca ccacgctcac tttcttgcca ccggtgatct
    11881 cttttaaccg ctcgaccaga ttctcttcgc gatagttaat aacctgccat gcgcccgctt
    11941 ttagcgcact ctgcgctttt tgcgcggttc ccacggtgcc gataagtttc gccccaaggg
    12001 cttttgccca ctggcaagca attaagccaa cgccgccagc cgctgcgtgg aacaggaact
    12061 gctcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt aagcctttca
    12121 ggaaggatgc cgcagcttgc tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg
    12181 cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag actacacgat
    12241 cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct gccgcttcgg
    12301 tacctaatcc gctgggtagc gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga
    12361 taaaattgat gccgatggct ttattttcga cctggatttc attctccgcc ggatcggcag
    12421 gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg
    12481 ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa agtttcgacc
    12541 cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc aacaaacagc
    12601 aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct ccgcactcga
    12661 tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgt tgggacgatg
    12721 tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgtcat atctttactg
    12781 aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt gcggaatcgc
    12841 tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca gagttgtcaa
    12901 aaaatacgcc aagtgcagcg aacatcagtg cctatgcgga catcgtgcgt gaacgtgccg
    12961 ttgttcgcga gatgatttcg gttgcgaatg agattgccga agccggtttt gatccgcagg
    13021 ggcgcaccag cgaagatctg ctggacctgg ctgaatcccg cgtctttaaa attgccgaaa
    13081 gtcgtgcaaa caaagacgaa ggcccgaaga acatcgccga tgtgctcgac gcaaccgtgg
    13141 cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggggta aacaccggtt
    13201 atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc atcgtcgccg
    13261 cgcgtccgtc gatgggtaaa acgacatttg cgatgaacct cgtcgaaaac gcggcgatgt
    13321 tgcaggataa accggtactt atcttctcgc tggagatgcc atcagaacag attatgatgc
    13381 gtactctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg cagctcgatg
    13441 atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa cgtaatatct
    13501 atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgcg cgccgtattg
    13561 cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg atgcgcgtac
    13621 cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctcccgctcg ctgaaagcac
    13681 tggcgaaaga attgaacgtg ccggtggtgg cgctgtccca gttgaaccgt tctctggaac
    13741 aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct atcgagcagg
    13801 atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac agtgatttaa
    13861 aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg acggtacgcc
    13921 tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag tacgacgacg
    13981 aataataatt attttatgaa ttaggtaatt aaagcaaaca cttatcaagg aacacaaatg
    14041 caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca acgtcttcgt
    14101 gaactggctc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta tggtcacggt
    14161 cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa
    14221 gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact cgaaggcttt
    14281 tttgatgcca gagatctacc gacgatttct gcgcaacatt ttcataccgc cgtgcataac
    14341 gaagaacagc ttgctgcgct ggaagaggcc agcctggacg agccggttac cgtctggatg
    14401 aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctga ggcgttttat
    14461 catcgcctga cccagtgcaa aaacgttcgt cagccggtga atatcgtcag ccattttgcg
    14521 cgcgcggatg aaccaaaatg cgacgcaacc gagaaacaac tcgctatctt taataccttt
    14581 tgcgaaggca aaccaggtca acgttccatt gccgcatcgg gtggcatttt actctggcca
    14641 cagtcgcatt ttgactgggt gcgcccgggc attattcttt acggtgtatc tccgctggaa
    14701 gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac ctccagcctg
    14761 attgccgtgc gtgagcataa agccggagaa cctgttggtt atggtggaac ctgggtaagc
    14821 gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc
    14881 gcgccgtccg gtacgccagt gctggtaaac ggtcgcgaag tgccgattgt cgggcgcgtg
    14941 gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggataaagc cggggacccg
    15001 gtcattttgt ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac aaaagtgagc
    15061 gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt ggattaatcg
    15121 ttctgtaata tttgattgtt tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa
    15181 atcctaccaa ttcaataagt tgatattctt tcatgctctt atgaaggccg tgcctctggc
    15241 ggatgtacgt ttgtcatgag tctcactctg ttgctaattg ccgttcgccc ctgaacatcc
    15301 tctcgatctt cgcctacttc cggtttattg tgttttaacc acctgcccgt aaacctggag
    15361 aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct tacgcttatg
    15421 gagcgtttta aagaagatcc tcgcagcgac aaagtgaatt taagtatcgg tctgtactac
    15481 aacgaagacg gaattattcc acaactgaaa gccgtggcgg atgcggaagc gcgcctgaat
    15541 gcgcagcctc atggtgcttc gctttattta ccgatggaag ggcttaacag ctatcgccat
    15601 gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca gcgcgtagca
    15661 accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcagattt cctgaaacgc
    15721 tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca cgtagcaata
    15781 ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc gactaactgc
    15841 gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag tattgtgttg
    15901 ctgcatccat gttgccacaa cccaacgggt gccgatctca ctaatgatca gtgggatgcg
    15961 gtgattgaaa ttctcaaagc ccgcgagctt attccattcc tcgatattgc ctatcaagga
    16021 tttggtgccg gtatggaaga ggatgcctac gccattcgtg ccattgccag cgctggatta
    16081 cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga gcgcgttggc
    16141 ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg gcaattgaaa
    16201 gcaacagttc gccgcaacta ctccagcccg ccgaattttg gtgcgcaggt ggtggctgca
    16261 gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga gatgcgtact
    16321 cgcattctgg cgatgcgtca ggaactggtg aaggtattga gcacagagat gccagaacgc
    16381 aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt aagtgccgct
    16441 caggttgacc gactacgtga ggagtttggt gtttatctca tcgccagcgg tcgcatgtgt
    16501 gtcgccgggt taaatacggc aaatgtacaa cgtgtagcaa aggcgtttgc tgcggtaatg
    16561 taatgcagga aagcaggctg gagttatcca gcctgcagtg aaattaaact gtcgtcgctt
    16621 tcactcttgc tttatagatg atttttttga tgccatcgtt ctacgtgaga ggtaataaaa
    16681 cgttgttagt tctaacttgt taagtttatc ccaatgttct ggagtacttt tccctgtttt
    16741 accgtgtgga gtaataattt ctgcacatct aaccattatt aaatttaata ataagtatcg
    16801 ggtttgttac gatagaatat ttctatatcc tcaagaatat gctttgcctt attgcctgtg
    16861 actttctgag attcaattat agtatcgtta aattctaatg ttaaagagaa ctcttttttt
//
LOCUS       DAYPHN010000107        16727 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000107 DAYPHN010000000
VERSION     DAYPHN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.107"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            86..520
                     /locus_tag="ACG0BW_003248"
     CDS             86..520
                     /locus_tag="ACG0BW_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260174.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIEGILKAQGGE"
     gene            520..1353
                     /locus_tag="ACG0BW_003249"
     CDS             520..1353
                     /locus_tag="ACG0BW_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260175.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNSFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            1433..1777
                     /locus_tag="ACG0BW_003250"
     CDS             1433..1777
                     /locus_tag="ACG0BW_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260176.1"
                     /translation="MNISWQTEIAIYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            1768..2241
                     /locus_tag="ACG0BW_003251"
     CDS             1768..2241
                     /locus_tag="ACG0BW_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260177.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKAGFTRG"
     gene            2243..2626
                     /locus_tag="ACG0BW_003252"
     CDS             2243..2626
                     /locus_tag="ACG0BW_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFK7260178.1"
                     /translation="MFIEAFASLMQKAKIGTVGADIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIPVEFAGYLLN"
     gene            2701..3447
                     /locus_tag="ACG0BW_003253"
     CDS             2701..3447
                     /locus_tag="ACG0BW_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFK7260179.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QLGQTVISELVQGRNITITAPLAESVLQNMVDLMPGSTLSEDDNSVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGKMS
                     GPKPVKTVSISPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINSAG"
     gene            3503..3820
                     /locus_tag="ACG0BW_003254"
     CDS             3503..3820
                     /locus_tag="ACG0BW_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260180.1"
                     /translation="MTKLLDLDSILPPKKSIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            3946..4170
                     /locus_tag="ACG0BW_003255"
     CDS             3946..4170
                     /locus_tag="ACG0BW_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260181.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            4178..8749
                     /locus_tag="ACG0BW_003256"
     CDS             4178..8749
                     /locus_tag="ACG0BW_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFK7260182.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGRRS
                     GASGHSGKSNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRV
                     MLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLKA
                     LVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGIT
                     MGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFADR
                     VGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQELV
                     INLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNRA
                     ARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMTM
                     LRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNSA
                     GVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAENQ
                     IFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSLS
                     SRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFERD
                     NAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFEG
                     KPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRSA
                     NASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTD
                     LANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPEADSIISA
                     GYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIKE
                     TGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITN
                     MVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGEA
                     TKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQ
                     NLSSVTGALSAAFAMLGANASGSGNKWLNFGATVASGLVSVWAGGGFDGLMSGSSGST
                     SGFNNLTSSAADGTNGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGD
                     MNEAYVPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNA
                     AQRMKAIALETIAQEKRPGGSLNPNTQRN"
     gene            8792..9127
                     /locus_tag="ACG0BW_003257"
     CDS             8792..9127
                     /locus_tag="ACG0BW_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260183.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERAGVESFYWVNPRGKRIVVVCDSHTVKRYQGYAVLT
                     APFRQVFEA"
     gene            9210..9908
                     /locus_tag="ACG0BW_003258"
     CDS             9210..9908
                     /locus_tag="ACG0BW_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFK7260184.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFANG
                     VIHFGGFIGATRYG"
     gene            9901..10698
                     /locus_tag="ACG0BW_003259"
     CDS             9901..10698
                     /locus_tag="ACG0BW_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFK7260185.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDKNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            10686..11279
                     /locus_tag="ACG0BW_003260"
     CDS             10686..11279
                     /locus_tag="ACG0BW_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFK7260186.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVVMPALAPLGLSLMMGGISQIISPQATNESVRQADNSNSYYFD
                     GPQNTENQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            11297..16024
                     /locus_tag="ACG0BW_003261"
     CDS             11297..16024
                     /locus_tag="ACG0BW_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260187.1"
                     /translation="MEQFKKKKLPLFIAGAGGKKSSKSSSRTPVEADDTVNSRAMAAI
                     LDLLGEGVIGGLVNGARSIFIDDLPIVNEDGSSNFSGITWDFRDGSQDQTPMSGFDFV
                     ETPKSVNIQLKKTHYVTVSIDNDEADRVRVIMKFPSLRSIDKKTGDTNGTTVEYKFQI
                     ANGDSTFVDVVAEGEKSVGIKLTAKKTGVYYRSYELKLPKPGRAYKVRVVRITDDNSS
                     QYLYNDTWVDSIGEIVDTPMNYPNSVLVGLKVNSEQFGSTMPSRSYLVRGLKIRVPSN
                     YNESSNTYAGVWDGTFKLLSSSNPAWILFDLLTNARYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVAFTKNSVTLDAPVSLAKGGCFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLTGKITSSELGQALL
                     EDINSKASQEAVDELNEHINQSVESLEGAVNDVKEDIAELDKQFSDNLADFEIKFNER
                     SDALENAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIK
                     AARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAE
                     LTTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEI
                     GEDIKASILEESTARADGDEALAKRVTQLQSKFEGDISAAISTEQEARTSADEALTKQ
                     ITQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDDDISAAIRSEQEARAS
                     GDSALAKQINQLQAKVDGDISAAITQEQEARASADEALSREINSLRAQTGTDIAAAVA
                     VETKARTDADSALSSQITSLTAKANDLEASLAKETTARADGDTALTKEVSSLKAQTAK
                     DISAAVAVETQARTDADSALSSQITKLTSQYKEDIKAAVATETKTRTEQDAALATQIT
                     NLESQTAANISAAVTTETTARTQADNALSGRIDTLKAEVDGNTATIQQQATAIADTNK
                     KVSTAWTLKMETSTSGGQKYVAGIALGIDTTGLSQFLVQADRFGLVNSVNGKITTPFV
                     IENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSGRSGWRFGKNGTLEINGNSGGNGRL
                     VINGQRIDVYDDNNVLRVRIGRL"
     gene            16118..>16727
                     /locus_tag="ACG0BW_003262"
     CDS             16118..>16727
                     /locus_tag="ACG0BW_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260188.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVIGPDNKL
                     YEIKRVISDTNIVLSEPYTGETQSEVPCRIITTYEGDLTQFSARFTALMSRMSADSKS
                     MRSWLTALDEVTIEREDGTEVTVKPLMQIVNEHNENVEWYKNNTDAIDAAGDKAREAA
                     ASAAAAAESANTAGEKASQASQSASAAASSQSAASASATAAKK"
BASE COUNT         4691 a         3645 c         4422 g         3969 t
ORIGIN      
        1 aagcgacagc gaccgtagaa gtcactgctg cacaggaata atcaatattg ggcggcgaaa
       61 gccgcccatg tagagagaat gcgttatgaa accagcaaaa attgttttat tagaaccgca
      121 attttccggt tattcgggaa tgttgtgcgg cgttcagttc gagaacgggg tgtccgtagc
      181 ggagttgcct tttatcgatc agcaaaggat ttgtgcctca atgcgagcat caacagtcga
      241 gggcaaaaat gtttctccgt ctgccgcata cagtgatcgt ggcgagttga ccgcagacct
      301 gattaccgag ccagcagcac ctgacattgt gccaatgaaa cgtgggacac cagatgaacc
      361 ggccaaacag atccagactt tcacacgaga agagttggag tcaattgcag acaatgaagg
      421 cattgccggt ctacgtgtga ttggtaatca ggttggtgtc aaagcgaaag gaattgtcga
      481 gatgattgaa ggcatcctga aagcacaagg cggtgagtaa tggcgcagat cgactcgtat
      541 cgtagcggtg aagctgtttc cctttcattt gccttcaacg tgctggatat cgaatctgct
      601 acctacacag taaaggacag caccggggct attctcgttg atggcgagcc gctagaaatt
      661 accagcggtc aaatgtcgat tccggttgtc gtgtcggctg aatataacca gctctctgag
      721 aaagagcgag atctgcggta cgtcattgtg aaggctgttg catcgggtct gacgcatgaa
      781 gagcggcaaa tgtatgttct gctgaatagt tttgaactgt cgataccaga acagtcgttt
      841 gcaactgtcg ctgatgctca aatgcaggcg atcgatatgc tgaatgggga cactttgctg
      901 tcggatggtg aaggcttaat gcgcaagcgt ctcattgagg ctaccagacg aattaaaact
      961 ttaccgttct caatccgcaa aattctacgt atcgactttg accgatacga tcgccctcaa
     1021 aatatgctga atgtgtatga cattccgtgg ggagcagatg gagcgtaccg gcatgattta
     1081 gtcgattggg aaaagatgac gcaggagaag tttgaagagt tccctgacta cttcaaagaa
     1141 gcgttgatgc ttgccgttgt caacgaagcg tgtgagatcg caaatggcaa tgatgtagcc
     1201 gcagcacgag aggatggcat tttgtcagag tccatcggtg aaacaaccaa catgtaccgt
     1261 accggtaaag ctgcaaacgt gcatgtggct cgtagtacct ggcgcttgct ggtcagttac
     1321 atcaacaacc gtatgattgt tcgccgtgcg taacgccagt cgcattattt acttttggtc
     1381 gaaagggttt ttggccagta atgagagcga ccgacaacaa cagggagaga gcatgaatat
     1441 ttcatggcaa acagaaattg cgatctaccg ctttggcgca aagaacgtat acggcgaggc
     1501 gcaattacaa ttcgtcagga agacgaatgt cggcgtggtt aagttcgaac agagtaatga
     1561 gaaatcgtca gttcgtgctg atagctctgg tagtcgtggt aaggcgagtc tggagctgtt
     1621 tgatgctgtg ttagttgtcc ctttagaagc ggctgtacaa cttgatgatg tgctgatcct
     1681 tgaaggtcaa aaattaaagg tgtccagtgt tcatcgccgc tgggggctac gaggaagacc
     1741 tgggcatctt gaagtggggg cgaatatatg ggtctgaaat acgatgcgca tcagttcaaa
     1801 cgtgcgggga atagactcaa taacagccag aaagcattta agcgatatct tattcgggac
     1861 atggaaaagc tggcgcgttt ggttgagcgt ctcagccgtg caatggcccc gctggaaacc
     1921 ggatctctgg agacagccat ctttgccagg gtgattaaag aaggttattc agggctgcgc
     1981 attgagcttt ctgtgtctgg agcaaaacca cgtcaggggc atccgggcgt agaagtcggt
     2041 gactatgcta agtacatgga gctgggtaaa taccgactcg gttatctttc tcgaatgaag
     2101 aacgtgacaa acccaccaat tgccggtgtt aaacctcgtg ttgggccaca ctttctggaa
     2161 agagcggtgg aaattagcga gaagcagttc tcagaagcga ttcttgaggc tgccagaaaa
     2221 gccggtttta cgagaggtta atgtgtttat tgaagcgttt gcaagtttga tgcagaaggc
     2281 aaagattggc acggtcgggg ctgacatttt ctgtcactac ttgcctgcca atgtgaaatc
     2341 tggcgtcctg cttattaacc caaataccgg tatcagcatc gatcatgagc tacagggctt
     2401 ttaccacgaa tcattcacaa taattgtgcg tggttcgtca attactacga cggttgagaa
     2461 agccaataaa atcattgaga tgtttccagt agaggaaacg gaatctggtg gtgtttattt
     2521 tcgactggta cgaccgatgg cgatgccaat catttatccc aaaaatgatg gagcattaat
     2581 agaagcaggc attcctgttg aatttgctgg ctatttattg aattaagtta atcggtaagt
     2641 atatacttat tgttagcgca atgaatgcgt agaattaacg gaaaaaggag ttttccatca
     2701 atgtctaata cccatgtaaa aaacatcaaa cttggtgcct gtaaggtgtc gtttggtggt
     2761 gtggatttgg gttacaccaa aggtggtgtt caggttgaag tagccactga aacgctgaaa
     2821 gtcaccgtcg atcagttggg gcagaccgta atttccgaat tagttcaggg gcgcaacatt
     2881 accatcaccg cgcctctggc tgagtctgta ctccaaaaca tggttgacct gatgcctggt
     2941 tctactctga gcgaagatga caactctgtc acgatcacat ctgcacaggg cgttaacctg
     3001 attgatgtgg cgaaggagct ggttctgact ccgcaggaca ccaccgacta tgtgttgacc
     3061 atcccgaaag cggcaaccgc gggcaacttc actatgacct accagtctga tgatgttcgt
     3121 gtgttctctg ttcagttcag cgcatacccg gatgatgaag gcgttctggg caaaatgagc
     3181 ggtcctaaac cggtaaaaac tgtgtcgatc tcaccggaat ctccaaccgt aaaagctgga
     3241 gaaactgtgc aactgacagc cgaaatcact ccagctgatg ccgcagacaa aaccggtgtt
     3301 tgggaatccg aagaccagga aaaggccacg gtagatcaaa cgggtctggt acgtggtgtg
     3361 gcgcagggtt ctacaaatat ctccttcacc agcaatagcg gtggtaagaa ggcaactaag
     3421 tcagtaacga ttaactccgc aggttaatca gtaattaagc agaggctcag gatgagcctc
     3481 tcttttaaaa ggattttaac caatgaccaa attactcgat ctcgattcca ttctgcctcc
     3541 gaagaaaagc atcaaatttg gcggtcagga atatcccatc gttgaaatga cggtaggtct
     3601 gttcgtttcc atcaagcaga tggaaggcaa agatctcatg aacatgtctc ctgttgagca
     3661 agtgacagct tatgcagatc tggttcgtaa ggttatccct tcagtacctg acgaagttct
     3721 tgaaaaactg actgttccgc aactccagca gatcttcacc ttcgctatgg aagtgattga
     3781 tgaagaaaac gaaaaagcgg ctggcgaagg ggcaaagtaa tttcccgcga tgaatccggg
     3841 acaaggaccg tctcaataga tctcggattc tatttcagtc gtgtagttgc tcactacgcc
     3901 gtgtcgccat tagagctact gaacgttcct ctcacgatgt tctggatgct cagtcgcaat
     3961 atagaccgtc tgcgtgcgga agaggatgtc cgcaacttac aagtcgctcg tgcgtcccag
     4021 gcggatggcg aggctgtgaa ggcgttcatg gagggtttgc aactcaggat tggaagacca
     4081 gtcgtaacag ataaagtcta cgatccaagc caggataagg cagaccctga cgccaaagag
     4141 caactgatgc aaatatttgg cagaggatga caagggaatg tcacaaaacg tagagtttat
     4201 cctgtcgctg gaagacaaac agtttacagc gtcaatcgat cgtgcgggga aactgcttac
     4261 tcgatttggt gagcaggtaa caaagcctgc tcaaaaaatc caaacctttg aacgctcttt
     4321 gggttcggtc gcccgtatca ttggcgttct ggaaagcaag ctcgattcta cggcagataa
     4381 actacaggat gtagctgccg gttttgagct tgcttcagat gctacgcgta aaatgcgtgg
     4441 caacatcacc agcctcaatt ctggtcttaa agccctgatt gagcgcgtcg atacgacaac
     4501 ttcttcagta gataaactca ccgcgtcatt acgtaaagtg caatcagagc taaatgattt
     4561 ctctgattgg gcgacctatg caagcaaaag cgcaagccgc tttggtacgg aggtcaaaga
     4621 agcctctgcg tccgtgagtg gcatgaatac gcgccttaat accacgacga agcgactcag
     4681 taattggggt gtcacaacga gtcaggctgc cgagggactg aaaaaggttc gtgagcagat
     4741 ggacgaagtt atcggacgtc agcaactgat tagcaagccg gtacgcgttc gtacatctgg
     4801 aagtggtgat ggtggtagcg ggcggcgtag cggcgcttct ggccatagcg gtaaaagtaa
     4861 cgaaggaggt atgttctctg gcctgcgtgg caacattttc ctgcttggcg agatcggaga
     4921 tgcagccaga acggttactg acatcatgtt tgggtggcag aagcctattg ttgaagctgc
     4981 ggccgaaatg gaacgtatgc gggtgatgct tcgagggttg aataaggaga agtccaaccc
     5041 gggccaggct gccgctgatg atatgaagta catcgtagac atggctcaaa atgcgccgtt
     5101 cgcgatgcag gcgttaacag actccttcgt gaagtttcgt tctgctggct tagatccaac
     5161 cgatggttct ctgaaggcgc tggtggactc cgttgctcgt tttggtggtg atagtgagct
     5221 gttgaaacgt gcggctgtgg ccgttcagca gatgtccggt aagggcgttg tgtcaatgga
     5281 agagctacgt cagcaattag gtgaagccgt tcctaacgcg atgcaggcaa tggcagacgc
     5341 cgcaggcatc actatggggg aactgactaa agccgttgcc agcggtacgg ttgaggcaaa
     5401 acaggcgttg tctctgatgt ttgttggtct gcgtgcggag aatgagaacg cagcgaaaga
     5461 catgatgcaa acctacacag gtgcgctggc gcaacttcag acgtcattca cgttatttgc
     5521 tgatcgggtt ggtcaggccg gatatctgga ttctctatcg aaggggatga aagaactggc
     5581 ttcaatcatg aatagcgccg aagggatttc gtttgccaat tctttaggtt cggggttaac
     5641 aacggcaatc gatgggttgc gtcagcttgc tcaatggtta gcaaagaacc aagagctggt
     5701 aattaatctc ggtaaggtcg tggccgcgat ggttgcgttc aaactgatgc gagcagggat
     5761 catgggggta gttggcgctg ggagccagat ggttagcacc tttgccacga tggcgaccgc
     5821 catacagact ccatttaacc tcggcgctac agcagtaact cgattcaatc gtgcggcacg
     5881 tatggggctg gctccgatcc cctctcttat tttcgccatc cgtggggcga ttacggggct
     5941 taaaggcgct tttgctggat taacggcgtt cattgcagca aaccctatag gggcagcatt
     6001 tacagttgcc actgtagctg ttgctggcct aatcacgtac atgaccatgc tccgcagcga
     6061 aacttcaaag gtcgttgacg agattaggaa aataccagaa gcgatgacag cggccaaacg
     6121 tgcacaaatg gccgaccgcg cagaaaagct cgaaaagcag attcttgctg atcaacgagc
     6181 attaaaaact ggtgagggtg ttaattacgt atctaactct gccggtgtta ttactcacaa
     6241 agagtcgaag gctgacatcg aagcacgcct gaaaaaaaat caggaggagt atcaaaagat
     6301 aaccggcacg atagctcttg gtgacggcgc tgtagcaaag cgtttggcta aagaggctgc
     6361 cgaatctcag attgagaaaa ttcgagcgga aaaccagatt ttcgcggcaa cattcgtgaa
     6421 agcccggcaa gaggctctgg ataagatcca gaaaatcaat gatgacggtt cactttcaga
     6481 tgacgaaaag aacaagctat tggcaccgtt acgtgaaacg gtaaacaaaa gctatctgga
     6541 gcctgcgcaa aaactggttg attcactttc ttctcgtaag aatgcgaccg agaagcaaat
     6601 tgcgactctt aatgatcagc ttgaaaaagc caaaaaggat ggaaataccg agcagatcca
     6661 gaaactgcaa ggcagtattc gtggttatca ggagcatttg gaagccgttg ctcaggagct
     6721 gactcaggca gagttcgaga gagataacgc ggccaaaact ggtaagggcg taatgtcaaa
     6781 ccaggggact gttcttgggt taggtacaac tgataaagct gctcagaagg cgctggcgca
     6841 atatatgcga aaccagatgg attctgcgac ttatcaacgt actttgcctg acggcactcc
     6901 gatgatggac ttcgaaggta agccgattat tgggcctaaa caactcaaga cgcagcttaa
     6961 tttgcagaaa gcatccagcg ccagctctct ggagaaaatg agcgatgaag agcgtgccgc
     7021 agccattgct gcactgacta aagctcgtga acaggatgcc gcagccgccg agaaagcagc
     7081 ccaacgatca gctaacgcct cgcagcgtgc ggccaagaag gaacaggcag cgcaacagaa
     7141 actggcagcc ggataccaga aggctctgga caaagccgat cagcttatgg ggcaaatggg
     7201 tgaaagctct aaggctacgg tatcgtttga tcagtctctt cgcgatacaa cgaaatcgct
     7261 gacagatttg gctaatgcgg ttcctaacga gttcatcact caagagatga tcgacaaggc
     7321 gaaaaaacgt cttgaagacc tcaaaaatgc gacacctgaa tatcgcgaga tgtttaatcg
     7381 ccgcaatgtt gagcagatga tctccacctg ggcaccggag gcggattcca ttattagtgc
     7441 tggctatacg ccgtctcgcg aagagaaagt tgctgatttc gaagacacct acaaccgcaa
     7501 tctcaaagcg ttgatagaac ttcgtgataa ggcgtctgat cctaaagttg tggcgcttta
     7561 tacaaagaaa atcaatcaac tgattgctgc tggcaatacc gcgcttatta aagagacggg
     7621 gactgcgacg cagaagttgg cactggaata cgaaaacctg gcagagcaga tcgaaagcac
     7681 ctggactgat ttgtttagtg gcttaactga tgtcctgact gatttcgtta ttaacgggaa
     7741 gatgagcttc tccagcctat ctcagtccat tttgaaagat atcaccaata tggtcgtgaa
     7801 gtcgcaaatc acgctgcctc taatgaacat gttggggatg ggaaccaccg cagctggtag
     7861 ttcacagagt ggcaatttgc tcactggagt tgcttctgcc gttgccaatc aaggtgtacg
     7921 aatgggcaac actgttaatg gcgacaagtc ggtaggagaa gccacgaagg agacgtccag
     7981 ttcggtaact ggattggggc aaacaacaca gcagaccacc agcgcaattg gcacagcaac
     8041 aaatgcgatt ggtagctggg tatcggggct atttgatagc accgaagcca aagatgctga
     8101 gacaaaagca gtgaaggact ccatcttctc gatgcagaac ctcagctctg ttaccggcgc
     8161 tctgtctgcc gcgtttgcaa tgcttggagc taatgcttcc ggctctggta ataagtggtt
     8221 gaatttcggc gcgacagttg catctggttt agtttcagta tgggctggtg gtggattcga
     8281 tggattgatg tccggttctt ctggctcaac ttccggcttc aacaatctga ccagttctgc
     8341 tgccgatggc acgaatggta ttcctgcaat tccgaagttt gcaaatggtg gcatattcgg
     8401 aaaagacggc gtgatcccgc tccgggcata ccagaaaggc ggtatcgcca actcacctca
     8461 attggcgttg tttggggaag gcgatatgaa tgaggcgtat gttccattgc ctgatggtcg
     8521 aacaatccct gtaacgctca gtaccgatgg tatgagtgga ggaggaaatg ttctttctcc
     8581 ggtatcaatt gagatcaacg tccatagtga cggtagcaca actgaatccg gcgatacaga
     8641 aagcatatgg aacaatgccg ctcaacggat gaaagcaatc gcgcttgaga ctatcgctca
     8701 agagaaacgc cctggcggat cactcaaccc aaacactcaa cgtaactaac tatcgactgc
     8761 cccggtcggg gcagtctcac aaggatgtga gatggaaaga caaacgttta attggtatcc
     8821 agattacgaa tctgaaaaaa gcgtaaaacc gaatgtaacg gtacttaatt ttggtgatga
     8881 ctacgagcag cgacaggctc aaggtcttaa tcgtattaaa gaagaatggt cgttaacctt
     8941 taccagatca tacaacgaaa ttaatgcaat cgatgacttc ctgactgagc gagcaggcgt
     9001 tgagtcgttc tattgggtta atccaagagg aaaacggatt gtagttgtat gtgacagtca
     9061 tacggtcaag cgatatcagg ggtacgcagt attaaccgct ccattcagac aagtatttga
     9121 ggcttaagta tctggataag taagtactaa tttactatca ttgtggcgct gacaggatgt
     9181 tagcgcctac ttatttcaag gatgaaacaa tgggaattaa agctgatatt cagagcttat
     9241 ctccctctgc actcattgag ttgttcgtac tggatatgtc gaacacaact tcagggggga
     9301 agctattctt ccacgccgga acaaacgaac tgatgcaacc ggtcgtctgg caaggagtga
     9361 catacgagcc gtggccaatc aaagcatcag gctttgacaa aacgggccag ggaacgttgc
     9421 cacgtccaaa aattcaggta tcgaactttg ccggaaccgt ctctgcggaa gttcaggcaa
     9481 acgacgatct tgttggctgc cgcattattc gcaagatgac gctggctcgc ttcctcgatg
     9541 ccgttaattt taaagacggt aatccaacag cagatccaaa ccaacatttc ccggatgaaa
     9601 tgtggtttat cgaacagaaa acgctcgaaa ctcatcaggt tgtcgagttt gagttgtcca
     9661 gtgtgttcga tttgatgggg gtgcaactgc cgtatcgtca gatcattaaa aacacctgcc
     9721 cgtggaaata ccgagggcca gaatgcggct ataccggtcc atatttcgac aaaaataacc
     9781 agcagacgtc tatgtctggt gctgattact gcacaaaacg ttatgacgcc tgtaatgcgc
     9841 gtcggaatta ttttgccaac ggagtgatcc attttggcgg gtttattgga gctacgcgat
     9901 atgggtaata aagcaatccc tgagcttggc tctgatgtta tgcagcaaat ctatctctgc
     9961 gccataaatc gctaccctaa tgaagcgtgt ggctttctgg tgagaactaa tggcgacaaa
    10021 tatcgcttta tggaagcgcg aaatgtttcg gagaacccgc agaacacttt tgtaatgcac
    10081 gttgacgaca ttatggcggc agaggatgca ggtgacgtaa tcgcaatctg gcattcacat
    10141 actgatgaat cagcagaagc atctgatgcc gatcgtgcag gctgtgaagc gacggaagtt
    10201 ccgtggatga ttctggctat tcgcaagaat gttgagggcg atgccccttt ccattttagc
    10261 gagatgaatg tgatcacacc tgatggtttc gaaatgccat acctgggtag accgtatgta
    10321 tttggcgtat tcgattgctg gatgttgtgt cgggactatt tgaagcgtga gtttaacgtc
    10381 gagcttaacc cgaacgcaca cctgcatatt ccatcgtggt acaccggcga taacgacatt
    10441 ctcgacaaga actaccgaaa cgaaggattg gtacggcttg cgcccggaac agagcctcaa
    10501 cgtggggacg tcttctttat tcaatacggg aaaatgccag atcactgcgc ggtttatatc
    10561 ggcgatggaa tgattcttca tcaccagatc gaccgtctta gttgtcgtgc ttattacggc
    10621 ggaatgtatc agaaacatac gacgcatcac ttgcgtcaca gagacttgct caagggagat
    10681 gagacgtgtc tgagttagtt catgtgcagc ttggtggccc tatggccaaa cattttggtc
    10741 gccactggca tctaaaggtg cgcaatacaa aacaggctct ggatttaatt gaggccaaca
    10801 agccagggtt taaagcatgg atgaagcgca acatcaaaac ctatgaccgt taccacatcc
    10861 agatcaccaa taaacagggc cacaagtggt ctgttgacga aagtgaatat cagatgatgg
    10921 ggcagtctga caacattgcc aaaatccgca ttacacctgt tccgcgagga agcggtggat
    10981 ctgcttttgg gtggtttcag acggtagtag gggccgcttt gttggttgta tcggcggtag
    11041 tgatgcctgc tttagcacct ctcggtttgt cactgatgat gggcggcata tcacaaatca
    11101 tatcgccgca agccactaac gaaagtgtga gacaggcaga taactcgaac tcttattatt
    11161 tcgacggccc tcaaaataca gaaaaccagg gcaacccagt acaacttatc tatggcgagg
    11221 aaattctggt tggctcacag gttgtgagtt cttcaatcac gattgaccag ctaatgtaaa
    11281 caaggatttt ttgaacatgg aacagttcaa gaagaaaaag ttaccgctgt ttattgccgg
    11341 tgcgggtggt aaaaaaagca gcaaaagctc cagtcgtaca ccggttgaag ccgacgatac
    11401 cgtaaattct cgtgcaatgg ccgctatcct cgatcttctt ggggaagggg taattggcgg
    11461 cttagtaaat ggcgcaagat ctatttttat cgacgatctg ccgattgtaa atgaagacgg
    11521 ttcctccaac ttcagcggaa tcacatggga ttttcgcgac ggttcacaag accagactcc
    11581 aatgtctggc tttgatttcg ttgaaacgcc taaatccgtc aatatccagt taaaaaaaac
    11641 acattatgta acggtttcaa tcgataacga tgaagctgat cgggttcgtg tcatcatgaa
    11701 gtttccttct ttgcgaagca tagataaaaa aactggtgac acgaacggta cgactgttga
    11761 gtacaagttc cagatagcaa atggcgactc aacatttgta gatgtggtcg cggaaggtga
    11821 aaaaagcgtt ggcattaagc taacagcaaa gaagaccggc gtttattacc gtagctatga
    11881 gctgaagctg cctaagcctg gacgcgcata caaggttcgt gtagtccgta ttaccgatga
    11941 taacagtagc cagtatctct ataacgatac atgggtggat tcaatcgggg aaattgtcga
    12001 tacaccgatg aactatccaa actctgtttt ggttggattg aaggtaaact ctgagcaatt
    12061 cggcagcacc atgccatctc gctcttatct ggtgcgtggc ctaaaaattc gagtaccttc
    12121 aaactataac gagtccagta acacttacgc tggggtatgg gatggcactt ttaagctgtt
    12181 gtcttcttcg aaccccgcgt ggattctctt tgatttgctg accaatgctc gatatggcct
    12241 tggtcagtat gtgtcggaat ctatgattga ccttggccag ttgtaccaga ttggtcgata
    12301 ttgtgacgaa gaggtcgatg atggctttgg tggcaaagag aaacgctttg caatcaatac
    12361 gcagatcacc agtcgacagg acgcataccg gctaattcag gatattgctg gagcattccg
    12421 cggcatggtg ttttgggctg gtgggatggt taacatcatg caggatagcc catcagatcc
    12481 ggtaatgatg tttaccaact ctaacgtcaa agatggattg tttacctata aaggttctgc
    12541 gcgtaaagat cgcccatccg ttgcgctcgt aacctacaac aacaaggagg acggttataa
    12601 gcaaaacatc gagtacgttg aagatcagga cgcaatgcgc cgttatggtg agcgtaagac
    12661 agaagtcgta gcatttggct gtacaagccg agggcaggct caccgagttg gtttatggct
    12721 tttatatacc gccagaatgg agtcggatgt aattacattt actgccggct tagacgcgtc
    12781 atttctgatg cctggtgaaa ccgttctgat tcagaacaaa tatcgcgcag gcaaacgtaa
    12841 ctcaggtcga attgtggcgt ttaccaaaaa cagcgtcaca ctcgatgcgc ctgtgtcgtt
    12901 agccaaaggc ggctgcttta ttcgaatact gaatcaggaa ggcaaaatcg ttgaacgcga
    12961 tgttcttgaa actggcgaaa atataacaaa agttacgttt tcaaaagccc tgtcttcggc
    13021 tgaaacgcct gttttgaacg gcgtctggac tattacagaa ccagacctcg aacctatgcg
    13081 cgttcgcatc gttaacatcg cgcaggggga aacgccgggt agctttgaca tcaccgctgt
    13141 tgagaacaat ccgtctaaat atgaggcgat cgacaatggt gcaacgctta tcccgcaaaa
    13201 tacgacggta ttggacccga cttactccaa gccgtctaat ttgcaaatca ccgaagggac
    13261 ttatctctca agccctggca acctgtcagt aaaactgact gcaacatggg aagggaaatc
    13321 tccagagtat tggatcagtt ggcgacgttc tgatgaaaac aatgtatcga actggcaatc
    13381 ggcgcgtgta accgaagagc aatacgaaat cgttaatgtt gcggagaatg gacgctacga
    13441 cttccagctg tatgcggttt cattcaacgg taaaaaaaca gagattatca gtaccgttta
    13501 tcaggtgctg ggcacaatga caccgccaga tgccccaacg tcattaacgg ccgttggaga
    13561 ctatcgtaat gtgatactga attgggttaa tccagattcg gtagaccttg atcacattaa
    13621 tgtttacgcg tcccagacca ataatctgga tacggcgaaa ctgatcgcag agtctgcaag
    13681 caccaccttt acacatgctg gtcttgggga tagcgaaacg tggtattact gggttcgagc
    13741 atcgaacaag cgaggtatgt taagtcctcc gaactcaaac ttgggtacag aggcgacaac
    13801 tcgcgatgta ctgtcgttcc tgactggcaa aattacatct tccgagcttg gtcaggctct
    13861 gcttgaggac atcaacagca aagcctctca agaagccgtt gacgagttaa atgagcatat
    13921 caaccagagc gtcgaatctt tggagggggc ggtaaacgac gttaaggaag atatagctga
    13981 attagataag cagttcagcg ataacctcgc ggattttgaa ataaaattca acgagcgtag
    14041 cgatgctttg gagaacgcac aaaccgagct taaaggtgag gtttcggcaa cgatcgacaa
    14101 ggtcaatgaa gcgtttgaaa aaattgatgc tactgatgct gcaattgttg aaatcgaaaa
    14161 taccgtatct gaacacgata aagctctcgc taatacagtc gaggcaataa aggctgcaag
    14221 agatgaagcg gcggctctta ttgctaagga aagcgaggct cgtgttgaag gcgatgctgc
    14281 aaacgctaaa caactggaag tgttgcagtc aacggtagag gaaagctccg ctgccgttga
    14341 agaaatgaaa aagacggttg cagaggtcga tcgtgcaagt gcagaactga ctacgaacat
    14401 tgaggcgtta gccaaaacaa atattgacct ggctcttcgt caagatgaag accagcacaa
    14461 gcagatggtc aataacgcga agatcgcaac aacacagaaa acctttgctg atgatatgtc
    14521 tgcaatggct acgaaggttg aagaaattcg cgtagaaatt ggcgaagaca ttaaggccag
    14581 tattctggaa gagtccaccg cgcgtgcaga tggcgatgaa gcgttagcaa agcgtgtcac
    14641 tcagctgcaa tctaagtttg aaggagatat cagcgcggcg attagtacag aacaggaggc
    14701 tcgtacatct gccgatgaag ctttgacgaa acaaataact cagcttgagt cgaaagtaag
    14761 caacgatatt gtcgcagcca ttaaggaaga gcaagaagcc cgagcaacgg aagattcggc
    14821 tctggcaagc cagatcaccc aacttcaggc aaaagttgat gatgatattt ctgctgcaat
    14881 acgcagtgag caggaggctc gtgcgagcgg agactctgcg ttagctaaac aaataaacca
    14941 gcttcaagca aaggttgacg gtgatatatc cgccgccatc actcaggagc aggaggccag
    15001 agcaagtgct gacgaggcgc tctcacgaga aattaacagc ctgcgggcgc agaccggaac
    15061 tgacattgcc gcagccgttg ctgtagagac aaaggcaagg actgacgctg atagtgcatt
    15121 gtccagccag attacatctc ttacagcaaa agctaatgat ctcgaggcgt ctcttgccaa
    15181 agaaacaacg gctcgtgcag atggtgatac cgctttaaca aaagaggttt caagtttaaa
    15241 agcacagact gcgaaagata ttagtgcggc cgttgcagtg gaaacgcagg ctcgaactga
    15301 tgctgactcg gcgttgtcat ctcagatcac caagctgacc tctcaataca aagaggatat
    15361 taaagcggct gtggcaaccg aaacgaaaac gcgtacagag caagatgcgg cattggcgac
    15421 ccaaatcaca aacttagagt ctcaaacagc agccaacatt tctgcggcgg tcacaactga
    15481 aacgacagcg agaacgcagg cagataatgc tctaagtggg cgaatcgata ctctgaaagc
    15541 agaggtggat ggaaataccg cgacaattca acagcaggca acagctattg ctgataccaa
    15601 caaaaaagtg tcaaccgcgt ggacgttgaa aatggagact tcaacgagcg gagggcagaa
    15661 gtatgttgcc ggtattgcgc ttggtattga caccactggg ctatctcagt ttttggttca
    15721 ggcggacagg tttggtttgg tcaactctgt taacgggaag atcacaacgc catttgttat
    15781 cgaaaacagt atcgcctata tgaatggagc gtatatcaag gatggcacaa tcaccaatgc
    15841 aaaagtaggc gatctgcaat ctaccaattt tgttagcggt agatctggat ggcggttcgg
    15901 caaaaatgga acgcttgaga tcaacggtaa tagcggcggc aatgggcgat tggttataaa
    15961 tggtcagcgg attgacgttt atgacgataa caacgtccta cgagtaagaa ttggccgtct
    16021 gtgatggtgg aaaaaaaatt ttatatcggt aaaaataggt aagtatttac ttaatggcgg
    16081 gcaaggataa cccgccaaat caaggagcat attcaacatg tggtacaggg aaggtactat
    16141 cacatttaca cagggtagca atactctggt tggggcaggg acagcctgga acgtaacagc
    16201 taatggtgtg ttgccgggga tgatcgtcat tggccccgac aataagctgt atgagatcaa
    16261 gcgcgtaata agcgatacga atattgttct ctcagaacct tataccggcg aaactcagtc
    16321 tgaggttccg tgccgaatca ttacgaccta tgaaggcgac ttaacacagt ttagcgcgcg
    16381 ctttacagca ctaatgtcgc gtatgtcggc tgattccaag tctatgcgca gttggttgac
    16441 tgctctggat gaggtgacaa tcgagcgtga agacggtaca gaagtaaccg ttaagccgct
    16501 aatgcagatc gtcaacgagc acaacgaaaa cgttgagtgg tataaaaata acactgacgc
    16561 gattgatgct gctggcgaca aagctcgtga ggcggcggcc agtgctgccg cagcagcaga
    16621 aagcgccaat accgctggag aaaaagcctc tcaagcgtct caaagtgcat ccgctgcggc
    16681 atcatcacaa agtgccgcaa gtgcgagtgc aactgctgcg aaaaaat
//
LOCUS       DAYPHN010000108        16418 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000108 DAYPHN010000000
VERSION     DAYPHN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.108"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(58..210)
                     /locus_tag="ACG0BW_003263"
     CDS             complement(58..210)
                     /locus_tag="ACG0BW_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFK7260189.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(475..1209)
                     /gene="cysH"
                     /locus_tag="ACG0BW_003264"
     CDS             complement(475..1209)
                     /gene="cysH"
                     /locus_tag="ACG0BW_003264"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFK7260190.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1283..2995)
                     /gene="cysI"
                     /locus_tag="ACG0BW_003265"
     CDS             complement(1283..2995)
                     /gene="cysI"
                     /locus_tag="ACG0BW_003265"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFK7260191.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2995..4794)
                     /gene="cysJ"
                     /locus_tag="ACG0BW_003266"
     CDS             complement(2995..4794)
                     /gene="cysJ"
                     /locus_tag="ACG0BW_003266"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFK7260192.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDVLKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GIWYQNDPALVKELVELLWLKGDETVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5113..5475
                     /gene="queD"
                     /locus_tag="ACG0BW_003267"
     CDS             5113..5475
                     /gene="queD"
                     /locus_tag="ACG0BW_003267"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFK7260193.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5553..6824
                     /locus_tag="ACG0BW_003268"
     CDS             5553..6824
                     /locus_tag="ACG0BW_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFK7260194.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTQALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLALETSAIEERFHLENNEGAALLFSGGICDDLPGGT
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNIERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIMSLRCL"
     gene            6815..7075
                     /locus_tag="ACG0BW_003269"
     CDS             6815..7075
                     /locus_tag="ACG0BW_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFK7260195.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7092..7667
                     /locus_tag="ACG0BW_003270"
     CDS             7092..7667
                     /locus_tag="ACG0BW_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFK7260196.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7814..8674)
                     /locus_tag="ACG0BW_003271"
     CDS             complement(7814..8674)
                     /locus_tag="ACG0BW_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFK7260197.1"
                     /translation="MNIAIVTINQENAAIASWQAAQDFSGCTLAHWKIEPQPAVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(8671..9450)
                     /locus_tag="ACG0BW_003272"
     CDS             complement(8671..9450)
                     /locus_tag="ACG0BW_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFK7260198.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLELIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAAESPA
                     MQCRQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9428..10837)
                     /locus_tag="ACG0BW_003273"
     CDS             complement(9428..10837)
                     /locus_tag="ACG0BW_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7260199.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGIKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10859..12313)
                     /locus_tag="ACG0BW_003274"
     CDS             complement(10859..12313)
                     /locus_tag="ACG0BW_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFK7260200.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12383..13168)
                     /locus_tag="ACG0BW_003275"
     CDS             complement(12383..13168)
                     /locus_tag="ACG0BW_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFK7260201.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILINNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13487..14764
                     /locus_tag="ACG0BW_003276"
     CDS             13487..14764
                     /locus_tag="ACG0BW_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFK7260202.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRNTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14791..16269
                     /locus_tag="ACG0BW_003277"
     CDS             14791..16269
                     /locus_tag="ACG0BW_003277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFK7260203.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDNETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVENNAKRY
                     DAMNKGIFKELTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3869 a         4344 c         4157 g         4048 t
ORIGIN      
        1 gtgcaggaga ccaaaccatc acaattcaat acgcggggtt ttacccccgc gtggtggcta
       61 cttcttcgat tcgtaagcga gaactgcctt aaactccata ccacgttctc tgatactcaa
      121 ctcacacaac gagtcgccta ccatcagcgt gaatcccagt actgtacaac acagtacgat
      181 gattgccaca agggcatatt ttgtcagcat gtcttgcctc cctgcaaaga agagactaac
      241 attcgaattg cttaggttcg aacgctggcc tcaggttgat agaaatatcg cctggggctt
      301 ttgtccatct ggaacctcgc gaatgcttaa cgccagacag cctcaagcac ccaacgccat
      361 tctatacctg ataattcttc cagcggcgtt cacgtttaaa atcaggaatg ctgctcgcaa
      421 aataaaaacg cccccatcat ttctgacaga ggcgtttaat ttatccggca atatttaccc
      481 ttcgtgtaac ccacattctc tttttaagcc aaagaaacgc gtttcttctt ccgccatgcc
      541 gggttcccat ttacgggttg tatgggtatc accgaccgat aaatatcctt catcccataa
      601 tgggtgatat ttcaggccat gtttttgcag atactgatat atcgttcggt tatcccagtc
      661 gataatcggc agcactttaa atacgccacg ctgaattgcc agcaccggta aattggcgcg
      721 actgccggat tgttcgcggc gcaggccagc aaaccacgtt tgcgcattca gttctttcag
      781 cgcccggttc atcggttcga ctttgttgat gtcattgtac ttttcaatgc cttcaacgcc
      841 ctgctcccat agtttaccgt agcgtgcttc ctgccaggct gcgctttcgg tagcacggta
      901 cactttcagg ttgagtttga gtttgtccgt taactcgtca ataaagcggt aggtttccgg
      961 gaacaagtaa cccgtatcgg tgaggatcac tggaatatcc gggcgaatct gattcaccag
     1021 atgcaggctc accgccgcct gaatgccgaa actggaagaa agtacatatt caccgggcag
     1081 attatccagc gcccaggcaa cgcggccttc agcgtccagt ttttccagtt cggcgttagt
     1141 ttctgccagc gccagaatgc gatctacctt cggcagttcg ttcagggcgt ttagatcgag
     1201 tttggacata gattcctcac tgtttgcctt gcctgatgcg acgctcacgc gtcttatcag
     1261 gcctacaaga ccgggctgat ggttaatccc acaaatcgcg cgccggatcg agcaccgggc
     1321 gaatgatgcc cgcacgcacc gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg
     1381 cccagcgccc tatcagttca tcaagcgacg ccaggatttc cggttcggtg atgttttctt
     1441 tatacatccg tgggatacgt gtcccaatgc ggttgccgcc aagatgcagg ttgtagcgac
     1501 ccggcgcttt acccaccagg cccacttccg ccagcatcgc gcgaccacaa ccgttcgggc
     1561 agcctgttac acgcatcacg atatgctcat cgctgacacc atgtttcgcc attaaattat
     1621 cgatgttgtc gataaaagac ggcaggaaac gctccgcttc ggccatcgcc agcgggcaag
     1681 tcgggaatga cacgcaggcc attgagtttt cacgctgtgg cgtgacggca ttcattaacc
     1741 cgctctcttt ggcgatcttc tcgatcttcg ctttctcgct ttccggcact ccggcgatga
     1801 tcagattctg gttcgccgta atgcggaaat cacctttgtg gatcttcgcg atctccagca
     1861 ggccggtttt cagcggacgc ccaggataat caaggatgcg accattttcg ataaacagcg
     1921 tcaggtgcca gttatcatca atgcctttaa cccagccaat acgatcgccg cgtccggtga
     1981 actcatatgg acggatcggt tcaaatttga tccccgcgcg acgctccact tccgctttaa
     2041 acgtctcaac ccccacgcgc tccagcgtgt atttggtttt ggcattttta cgatcggtac
     2101 ggttacccca gtcgcgctga gtcgtcacga cggcttctgc caccgccagc gtatgctcca
     2161 gcggcagata accaaactca ctcgccgtgc gggcgtaggt tttcttgttg ccgtgctcga
     2221 tggaaagccc accgcccacc agcaggttaa agcccaccag cttgccgttt tcggcgatcg
     2281 ccacgaagtt catgtcgttg gcgtgcagat cgatatcgtt ctgcggcggg atcactaccg
     2341 tggttttgaa tttacgcggc aaataagtct gaccgaggat cggttcttca tcggtggtgg
     2401 cgactttttc ctgatcgagc cagatctccg catacgcgcg ggtacgcggc agcagatgtt
     2461 cagagatttt cttcgcccat tcatatgctt ccgcgtgcaa ctgcgactcg taagggttcg
     2521 aggtgcagag tacgttacgg ttcatgtcgt tagcagtcgc cagcgcatca agaccgaccg
     2581 agtgcagcat ctggtgcacc ggtttgacgt tctttttcag aatgccgtgg aactggaacg
     2641 tctggcggtt ggtcaggcga atgctgccat aaatggtgtt ttcaccggca aatttatcga
     2701 tcgcctgcca ctgtttggtg gtgatcacgc cacccggcag acggcagcgc aacagcatcg
     2761 cgtggcgcgg ctccagcttc tgttcagcac gttcggcgcg aatgtcgcgg tcatcctgtt
     2821 gatacatgcc gtggaagcgg atcagcagaa agttatcgcc cttaaagccg ccggtcagac
     2881 cgtcgtttaa atcttccgca atggtgccgc gcaggtagtt gctttcaagc ttcatgcgct
     2941 cggcgtctgt cagttttcct tcgaccacta aaggccctgg atgtttttcg ctcattagta
     3001 gacatctcgc tgataacggc gctctacgcg cagctcactt aaaaattcat ccgccgcttc
     3061 ggtgtccatg ccaccaaatt cggcaatcac ttccagaagt gcctgctcaa catctttcgc
     3121 catgcgatta gcgtcgccgc agacataaat gtgggcaccg tcattgatcc agcgccacag
     3181 ctccgcgccc tgttcgcgca gtttgtcttg tacgtaaatt ttttcttttt gatcgcgcga
     3241 ccaggcaaga tcgatacgcg tcagcacgcc ctctttgacg tagcgctgcc attccacctg
     3301 gtagaggaaa tcttccgtaa agtgcgggtt gccaaagaac agccagtttt tccccggcgc
     3361 ttcgtcggcg gcgcgttgct gcataaaggc gcggaacggc gcgatgccgg tgcctgggcc
     3421 aatcataatc accgacgttt ccggattggc tggcaggcgg aagttatcgt tatgttcgat
     3481 aaagacgcgg acttcaccct cttcttccac acgatccgcg aggaagctgg aggcaccacc
     3541 cgcacgggcg cggccttcca cgtcgtaacg caccacacca acggtaacgt gcacttcgtt
     3601 ctcgacttcc gcctgcgagg aggcgatgga atacaggcgc ggggtcagcg ggcgcagcag
     3661 attaatcagc gcttcggcat ccagttgcgc cggagagaaa cgcaccatgt caacaatcgg
     3721 cgtggtcgcg gcgtaatgct gtaacttcgc tttatcgccc accagcggca gcagcgtttc
     3781 gctgcgggta agcgtggcgt agttctcaac aatgttggcg gtgttgacgg tcagttcaaa
     3841 gtgccactgg agcgcttcgc tcagggtcag cgttttgccg tcaacggtga cagtttcatc
     3901 gcctttcagc cacagcagtt cgacaagttc tttcaccagt gccggatcgt tctgatacca
     3961 tatgcccagc gcgtcacccg gctgataacg caggccagag tcacctaagt caatttcgat
     4021 atggcgaacg tctttttcag agttacgccc ggtaattttc tggttaaccg aaaggctcgc
     4081 caccagcggc gcgtctttgc tgtacgggct ggtgtggatt tcatttaccg cgccagtagc
     4141 gacggattgc gaaggtgccg cgacaggcgc acgggacttc agaacatcaa ccacgcgggc
     4201 gcgccactcg atggcagcag cctggtattc aacgtcggca tcgacacggt cgagcaggcg
     4261 ttcaccgccc agttccgcca gcttgctgtc aaaatctttc ccggactggc agaaaaattc
     4321 ataagagcta tcgccgaggc taaacacggc aaatgcggtg ttttccagct tcggcgcttt
     4381 tttggagaac aggaacttat gcagcgcgac ggcttcttcc ggcggttccc cttccccttg
     4441 cgttgacgtc accacgataa gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc
     4501 cgcgttcacc agcttaacgt tcagttttgc cgccaataaa tcatcgcgta atgcttcagc
     4561 aacccggcgc gcattgccgg tttgcgaggc ggagataata gttatacccg gcatttctgc
     4621 ggcgggcgct ggcgtcgctg caagcgcagc aggctgctgg ttgagcacgc cccagaaata
     4681 gccagaaacc caggcaagct gggtgggagt taaatcggtc gtggccgcct gaaggcgtgc
     4741 cagttgctcc gggttcaacg gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg
     4801 ttatgttcca gtaagcaaag ctgtttctgc gccctgtcag cgcccataaa acagaagaga
     4861 aggtaaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
     4921 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aacagattaa ccaacccaat
     4981 gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc
     5041 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt
     5101 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
     5161 ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
     5221 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
     5281 ctaaaagcgg cgtttaagcc aacctacgag cgcctcgatc actattatct caatgatatt
     5341 cctggcctgg aaaacccgac cagcgaggtt ttagcaaaat ggatttggga tcaggttaaa
     5401 ccggttgttc cgctgttaag tgcggttatg gtgaaagaaa cctgcaccgc aggttgcatc
     5461 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
     5521 gggtgttgtg ttctggatat ctggggcatg acatggaaga cgactgcgac attattatta
     5581 ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
     5641 ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc gggcgtttat
     5701 atacccaggc gctcgcggaa ctcctcccac aatttcatct gaccgcgcct cttgaacgac
     5761 gcatcactca cgaaagcctt tccctgttaa cgccggatgg cgcaacgacg ttttccagct
     5821 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg
     5881 ccgaagccga aaaagaaggt gtcgaatgca tccccggtgc gacggtggat gcgctgtatg
     5941 aagaaaacgg cagggtgtgt ggtgtcattt gtggtgacga tattctccgc gcccgttatg
     6001 tggtgctggc agaaggagcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc
     6061 ctgctggcga agcgatggcg ttggggatca aagaagtgct ggcgcttgaa acatccgcta
     6121 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggga
     6181 tctgtgatga cttacccggc ggcacatttc tttatactaa tcaacaaacg ctctcgttag
     6241 ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctgg
     6301 ctcgctttaa aacgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt
     6361 atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta
     6421 acggctggct gctgctgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
     6481 gcggcatgga tatggcgctg actggcgcgc aggcggcggc acaaacgctg ataagcgcct
     6541 gccagcaccg cgagccgcaa aatctgtttg cgctttatca tcacaacata gagcgcagcc
     6601 tgctatggga tgttctacaa cgttatcagc atgttccggc gcttttgcaa cgccctggat
     6661 ggtaccgtgc atggcctgcg ttaatgcagg atatttcccg tgatttatgg gatcagggtg
     6721 ataaacctgt tccaccgctg cgccagttat tctggcgtca tttacgtcgt cacggcctgt
     6781 ggcatctggc gggcgatgtt atcatgagtc tgcgatgtct gtagcccgta atctctggcg
     6841 cgttgccgat gcgccgcaca ttgttccggc tgactccgtt gagcgtcaga cggtacaacg
     6901 gttgattaac gcttgtccgg ctggtctttt ttcgctcaca ccggaaggtg acttacgcgt
     6961 tgattatcgc ggctgcctgg agtgtggcac ctgccgtttg ctgtgcgacg aatcaacact
     7021 acaacagtgg cgctatccgc cttccggatt cggcatcacc taccgttttg gataaataat
     7081 aaggataatt tatgcccctc ttacacctgc tccgccagaa tccggtgatt gctgccgtta
     7141 aagataatgc cagcctgcaa ctggcaatcg actctgaatg ccagtttatt tccgtgttgt
     7201 acggcaatat ctgcaccatc agtaatatcg ttaaaaagat taagaacgcc ggaaaatatg
     7261 cttttattca tgttgatttg ctggaaggcg cgtcaaataa agaagtggtg attcagtttt
     7321 tgaaactggt gactgaggcg gacggcatta tcagtaccaa agcctcaatg ttgaaagcgg
     7381 caagagcgga aggttttttc tgtattcatc gcctgtttat tgttgattca atttcgtttc
     7441 acaacattga taagcaagtt gcgcaatcga atccggattg tattgagatc ctgccaggct
     7501 gtatgcccaa agtgctgggc tgggtgacgg agaaaatccg ccaaccgctg attgctggcg
     7561 ggctggtgtg cgatgaagaa gatgcgcgta atgcgattaa cgcaggtgtc gtggcgcttt
     7621 ccaccacgaa taccggggtc tggacgctag cgaaaaaatt gctttgacgg gataatcgta
     7681 acgaattgaa tttgtgtgat ttgtaggctg cacgccacat ccgacattca gcgtctgatg
     7741 cgacgcttga cgcgtcttat caggcctaca agtccccagg ccagaaccgt aggcaggatc
     7801 atccggcagc acgttattgg caatctgcgt gaatggttgt caccagtgcc tccagcaccg
     7861 ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa taccgccgcg ctggcgtcat
     7921 gattaatcgc caccacaaac ttgctattgc gtacgcccgc cattaacgct gctgcgccgg
     7981 aagcgccaac aacaatacac acctcaggtg ccagcaaatg tccggaaata ccaatcactt
     8041 tttcggcatc aacgccaccg ttcattactc gcgcccgact atagcccact tcggctccca
     8101 gcttttctgc cagcatagcg atttcctggt tatctgcctc tccgccctgc cccaccacca
     8161 gcacccgtct ggcctcagct aatgggtcac gggtaacatt tttcagatcc tctgtgctaa
     8221 ccagccagtc gggaagcgca cctgggacaa tgttcagttg ctgcatacca gagggcaacg
     8281 tggcgttttt cgctgctccc gcctgacgcg ccagggaaag gcatagcggg cgtttttctg
     8341 tttgcagcgt cgccgttagc gcatttcccc agtgggattt gcgcacgctt acggttggaa
     8401 tatctaacga agtcacctgg cagatgcttg caccatgtaa acgccaggcc agccgggtcg
     8461 acagttcatc gccaaacgtt cccggcggaa agagaacaac gtccgcaggc gttcgctgcc
     8521 actgttccac cagcgcatcc agcacctgtt ccgccacagc gggctgaggt tcaatcttcc
     8581 agtgcgccag ggtacaaccg ctgaaatcct gcgccgcctg ccagctcgct attgcggcat
     8641 tctcctgatt tattgtcaca attgcgatat tcatggctgc atcctttggc gtaaatagtc
     8701 ctgccatagc ttttgcgctt tttctgctac cgtttgccca tcaatcagcg tcgctccgcg
     8761 tcgttgctct gctcttgcca gttgccgaca ctgcatcgct ggcgattcgg cagcaacagt
     8821 tttacggata attgctgctt tccccgcagc cattcgctgg cgcattcccg gtactggcaa
     8881 ggccacttca ccacactgac gcacggcaat caccgcaggc aagcgaaccc ggcaacagcg
     8941 cagcccgttt tcagtacgct gttcaagggt gataaacggc gcgtcgagag taaaacgctc
     9001 cacctgggtg aagcagggcc agcccagcat ttccgccagc aaaaatggcg tttgcccatt
     9061 ctgcccttcg ctgctttggc agccagtaat tatcagctcc agcggattct ggtactgcca
     9121 ttcggcaata tggcgagcga caaattccgg cgcaaagcgc agatctgccg ccgtctccag
     9181 caatacagct tcctcaaacc caagagccat aagatagcgc agccagtgca acgcccgttc
     9241 atcccccatg ctcaacgcgg ttaaagacat cgacgtaccg ttttttctct gcgccaacag
     9301 cagcgcggcg gcagcctgtt catcagcacc gagcaaactt cgcagaagcg aaacatccgg
     9361 tccgctatta ccctgagccg ccgcctgcca ctctttttcc gccagcattc cggcatccgg
     9421 ttcggcttta aacgctaaca gaatgttcat tcgcacctcc aacatttccc gccgccacca
     9481 gcgggagtgc tttggtttct ggtgcccata gccaggtcac gacaaaacca accaacaaca
     9541 ctgccgccag gagcaataag gtgacttgca ttccccactg cgccagcacc cacggcagca
     9601 ggccagtact taccgccgcg cccaaccgac tcatggcggt ggcaaaaccg acgcccagcg
     9661 agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc caccagatta ctgactgccg
     9721 aaatggtggt gctgaagaga acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac
     9781 aagccatcac taccagcgtt gccgccagca gcaaaaaact tcccagcaaa aatttgcgat
     9841 gtgccagcag gtgcgtcaga actaatccca gcagcgcacc cacaattaac aacgcattaa
     9901 gcatcaggct agcggtcaac gcatcttcca gaccaatagt ctgggcgata gttggcagcc
     9961 aggtataaat cacaaaccat gggattacga ggcagacaaa gaagacgctg ttaaacgctg
    10021 tgcgccgcca gtagcgcgaa gagaacaagg ttttgatgtg tttatgggtc gccgttgcca
    10081 cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg cacgatagcg tgagcttctg
    10141 caaaacgccc ctggcgcagt agccagcgtg gcgattctgg cgttccccag cgtaataacg
    10201 taatcaacaa tgcgggcaga gctgccgatg ccagcagcca gcgccaggcc tccgggcttt
    10261 cgctaataaa gtgatgtccg gcaatacttg ccagcacata gcctacagtc cacaccacgc
    10321 tgaacgcgcc cagcaaaata ccgcggtggc ggcgcgggga aaattcagcc agcaaggtgt
    10381 gaccgactga gtaatcgcct cccagaccaa tgccaatcaa aatgcgcagt ccaataagat
    10441 gctctggcgt ggtggcaaaa aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga
    10501 aggtgaagat tttttgtcgc ccaatatggt cggagatcca gcccagaacc aggctgccaa
    10561 gaaacaaacc aaggagtgct gagccgccaa tcattcccgc cataaacggc gtcagttgca
    10621 tggcgggcgt aagctgaata atggcgtaac caatgacgcc gaggacataa ccgtcggtca
    10681 ggtgtgcgcc gaaagtgagc gcagcaatgc ggcagtgaaa acggttaagt ggtaaatcat
    10741 ccattcgcac cggtgaagtg ttcatcttta tcccttcatt gtccggtaat aagaacggct
    10801 tctcgcctga gaagccgaaa aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt
    10861 atttttcaat cggatagata gtacccgtat tcataatgcc gttaggatcg aactgctttt
    10921 tcagaccttc cagcaacgtc cacgcgctgc cgtgttccag cttgctccag tgaacgcgat
    10981 gtttaccaat gccgtggtgg tgcaccatcg aaccaccgag gcgaatggtt tcttcgcaga
    11041 tgattttgtt gagcggattg tggtacttgt cgatttcctc ttccggctta cagtcaacaa
    11101 cgttgtagtc gtaaacgaag tacatgttgg tgccgttctg gtagctgtga gaggagtgac
    11161 cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcggt acgaatacgg ttaataacgc
    11221 tttcgtagat ttcgtgaatg cagctccagc agccggacac ttcggtggta aagcccatgt
    11281 tgccggtttt gaggatctgc acacgttcgg cagctacttt atccggtccc cagttcaggt
    11341 tgttaaacca ggtttcgatc agcttgctgt ccactcgctg gcattgcggg taacgtgcca
    11401 cgatttcagc aatcccttcg cccgtcgcct tcgcaatgcg agggttacct tcagccataa
    11461 agatcagcac gcattttccg tcagcaaaat gggtgaagtg ttgggtgcca tcttcagcgt
    11521 catacaaacg agcgatcgac ggacgatacc cttccaccat gatttcacgc aggatgttga
    11581 agccggtttt catgtcttcc aggatatagc cgtagaagag gttgttttcc ggggtgaact
    11641 taaagatttt cacagttact tcagtgatat agcacaatgc accttcgttg ccgatgatga
    11701 tgtgacgaat gtccgggcct gccgcgcggc gtggtacgtt tttaatgcgt gttacggtgc
    11761 catcagccaa tactgcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg
    11821 agaactgccc gatactgcgg gttgctacca ggccgcccat ctgcgccagc ggctttgact
    11881 gcggagaatg ccccgtggtg taaccttttt cacgcaacgc gttttccagc acttccagcg
    11941 gaactccgca ttgcgccgtg gcctgcatat tctcaatatc aatattaatg atttgattca
    12001 tggcagagcc gtcgagcacc accgagtttt ccacaacggt ttccagccca ccttcggtag
    12061 cggaagcacc ggtacgcggc acaccgttaa ttttgtgcgc attcataaaa ttcagcacac
    12121 gggatacttg ttctgtggaa ccgagtttta cgaccgctgc cggaatcggc aaagtataaa
    12181 tgccatgaat atccggaaat ttgcgaaaac ggtcaatact gttcttcttt aatactgttt
    12241 catcggtaat tacgcgatct gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc
    12301 gagataaaga catagtaatt ccttcctgat aataaaaatg gaataatcat taataagaga
    12361 aaacagaaac caccgttatg gattagcgca ctaaataacc accatcaacc actaataaat
    12421 gtccgttgac ataattcgat gccggacttg cgaggaatac ggctgcgccc attaaatcct
    12481 gagtatcgcc ccaacggttt gccggaatat gatcaagaac gcgctgattg gtttctggat
    12541 tactgcgtgt cgccagcgta atatctgttg cataatagcc aggggcaata ccatttacct
    12601 gaatattata ttgacctagt tcatcacagt aagctttggt gaatccggca agagcatgtt
    12661 tagtggcaga atatgcaggt gaccattgtc cacctaagta agagaataat gaacagatat
    12721 taatgatttt accgcttttc tgcgggatca taatttttgc agcttcatag cttaactcga
    12781 atgcggcggt caggttcaca tcaatcatcg ggtcccagtc ggcacgaccg aagtccagca
    12841 ccttattcag cttacaaata ccggcattgt taatcagaat atcgacggta ccgaaacgtt
    12901 cgcaacaggc ggcgattatc ttctgcggcg cgccttctgc ggtgatatcc acctgcatga
    12961 agtccacctc aacaccctgt ttttcaatca tttcctttgt ttcgccgtta tctttaacga
    13021 agcttggaat aaagatattt gcgcctgctt tggccagcgc catggcaaat gcctggccta
    13081 aaccgctatt cccgccggta acaattgcgg ttttaccttt cagggagaaa aaatccattg
    13141 agaacgcatt gagagattcg attgacatag ttagctccaa tttccgtaag gcaaaaaaaa
    13201 agaaaggtaa cttcccccgc gagagggaag ttacctttct cattatctct ggtaactgga
    13261 gataattttt atcataaggg cataatcgct aatgtgatcg taatcacagt gttatattcg
    13321 aattgtcaaa taattttaaa aagcagaagc gtgattactc tcacacaaca ccactgcttt
    13381 ccatgttatt tcttattgca attactagag acctgagaaa agtaatccac ctgaaagggc
    13441 ggcttacttt tctctttttt ttgtctatta caaatgggaa tacgatatgc aacacaactc
    13501 atatcgccgt tggataaccc tcgcgataat tagttttagc ggcggcgtta gtttcgacct
    13561 ggcttattta cgttatattt atcaaattcc catggcgaaa tttatgggat tcagcaatac
    13621 cgagataggt ttaataatga gtacctttgg tattgcggct attattcttt atgcccccag
    13681 cggcgttatt gccgataaat tttcacaccg caaaatgatt acttccgcga tgatcattac
    13741 cggattactg ggtctgttaa tggcaacata tccaccgctg tgggtaatgc tctgtattca
    13801 ggtcgccttt gcgataacga cgattttaat gctgtggtcg gtgtcgatta aagccgcctc
    13861 gttgctgggc gatcatagcg agcaagggaa aattatgggc tggatggaag ggctgcgcgg
    13921 cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc ttttctcgct ttgcaccgga
    13981 tgacagcgcc agcctgaaaa cggtcattat tatctatagt gtggtttaca ttctgctggg
    14041 cattttgtgt tggttttttg ttagcgataa caacaacctg cgcaatacca ataacgaaga
    14101 aaaacagtca ttccagctta gcgacatcct ggctgttttg cgcatcagca ccacctggta
    14161 ttgcagcatg gtgatttttg gtgtcttcac catctacgcc attctgagtt actccaccaa
    14221 ctatctgacc gaaatgtacg gcatgtcgct ggtggcggcg agctacatgg ggattgtgat
    14281 caacaaaatc ttccgcgcac tgtgcggccc acttggtggc attatcacca cctacagcaa
    14341 agtgaaatcc cctacccgcg tgatccaaat cctttccgta ctcggcttgc tggcgttaac
    14401 tgccctgctc gtcacgaact ctaacccgca atcggtcgcg atggggattg gcctgatttt
    14461 actgctggga ttcacctgtt acgcctcacg cgggctgtac tgggcctgcc ctggcgaagc
    14521 gagaacaccg tcttacatta tgggcaccac ggtaggtatt tgttcggtga ttggattcct
    14581 gccggatgtc ttcgtttacc cgattatcgg ctactggcaa gacaccctgc ccgctgcaga
    14641 agcctaccgc aatatgtggc tgatgggcat ggcggcgctt gccatggtga tcgtctttac
    14701 ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc cctgctatgg ctagcagcaa
    14761 gtaagtccag aaaattacag gagaaaaatc atgtcgaaaa aatacatcat agggattgat
    14821 ggcggaagtc agagcacaaa agtggtgatg tatgatctgg aaggtaatgt ggtttgcgaa
    14881 ggcaaaggct tattgcagcc gatgcacacg ccagatgccg ataccgcaga acatcctgac
    14941 gacgatttat gggcatcatt atgttttgcc ggtcacgatt tgatgagtca gtttgccggg
    15001 aataaagaag atattgtcgg tattggtctg ggatccatcc gttgctgccg tgcgttattg
    15061 aaagccgatg gcacgccagc tgcgccgttg attagctggc aggatgcacg cgttacacgc
    15121 ccttacgaac atacgaatcc tgacgtggca tatgtcacct ctttttcagg ttatctgacg
    15181 catcgcttaa ccggcgagtt taaagacaat atcgccaact attttggtca gtggccggtg
    15241 gattataaga cctgggcatg gagtgaagat gccgcagtca tggagaagtt taatatcccc
    15301 cgtcatatgc tttttgatgt gcaaatgcct ggcaccgtcc tcggacatat cacaccacaa
    15361 gccgcactgg cgacacattt cccggcagga ctgccggttg tttgtaccac cagtgataaa
    15421 ccggtagaag ctctgggggc cggattactg gataatgaaa ctgcggtaat ttctcttggc
    15481 acttacatcg cattgatgat gaacggcaaa gcactgccga aagatccggt ggcgtactgg
    15541 ccaattatgt cttctattcc gcaaacattg ctgtatgaag gttacggtat ccgcaaaggc
    15601 atgtggacag tgagctggct gcgcgacatg ttaggcgagt cgttaattca ggatgccaaa
    15661 gcacaggatc tttcaccgga agatttactc aacaagaaag cgtcttgtgt gccgcctggc
    15721 tgtaatggtc tgatgacggt gctggactgg ctgaccaatc cgtgggaacc gtacaaacgc
    15781 gggattatga tcggctttga ttccagcatg gattacgcat ggatatatcg ttcgatactg
    15841 gaaagcgtgg cgctgacgct gaagaacaat tacgacaata tgtgtaatga aatgaatcac
    15901 tttgccaaac atgtgatcat tactggcggc ggttcgaaca gcgatctgtt tatgcagatt
    15961 tttgccgacg tgttcaacct tccggcacgc cgtaacgcca ttaatggttg tgcaagtctg
    16021 ggggcagcga tcaatacggc ggtaggtctg gggctatacc cggattacgc aacggctgtc
    16081 gacaaaatgg ttcgcgtgaa agatatcttt atgcctgttg agaacaatgc caaacgctac
    16141 gacgcgatga ataaaggcat tttcaaggag ttaaccaaac atactgatgt aatcctgaaa
    16201 aaatcgtatg aagtgatgca tggggaattg gggaatgcgg attcgatcca gagctggtcg
    16261 aatgcgtaag tgaggaaggc cgggcgggaa actgcccggc atgaacgtac ctgaatggtt
    16321 atccccgctg acgcggggaa cataagttcg acaacttaaa aaatgataac aagtccggtt
    16381 tatccccgct gatgcaggga acataataaa aactttaa
//
LOCUS       DAYPHN010000109        16177 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000109 DAYPHN010000000
VERSION     DAYPHN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.109"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            48..2225
                     /gene="sinH"
                     /locus_tag="ACG0BW_003278"
     CDS             48..2225
                     /gene="sinH"
                     /locus_tag="ACG0BW_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFK7260204.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQTAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGKDGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
     gene            2280..3257
                     /locus_tag="ACG0BW_003279"
     CDS             2280..3257
                     /locus_tag="ACG0BW_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFK7260205.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNEDARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            3378..11420
                     /locus_tag="ACG0BW_003280"
                     /pseudo
     CDS             3378..11420
                     /locus_tag="ACG0BW_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
     gene            complement(11480..12856)
                     /gene="xseA"
                     /locus_tag="ACG0BW_003281"
     CDS             complement(11480..12856)
                     /gene="xseA"
                     /locus_tag="ACG0BW_003281"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFK7260206.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     AQQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLWLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKAGDTLTTRLGDGVVISEVSAVTKTRK
                     PRKKAANP"
     gene            13018..14484
                     /gene="guaB"
                     /locus_tag="ACG0BW_003282"
     CDS             13018..14484
                     /gene="guaB"
                     /locus_tag="ACG0BW_003282"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFK7260207.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14553..16130
                     /gene="guaA"
                     /locus_tag="ACG0BW_003283"
     CDS             14553..16130
                     /gene="guaA"
                     /locus_tag="ACG0BW_003283"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFK7260208.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEARFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
BASE COUNT         4125 a         4060 c         4371 g         3621 t
ORIGIN      
        1 aatattaaag cttatttttg actgtatcat tgtcacaggg aaagtgcatg ctgcgatgga
       61 aacgctgtat tattctaaca tttatctctg gtgctgcttt cgcggcacca gagataaatg
      121 ttaagcaaaa cgaatcgtta cctgatttag gtagccagac agcacagcag gatgaacaaa
      181 ccaacaaggg taaatcgctg aaagagcgcg gagccgatta cgtcatcaac tccgccacgc
      241 aagggtttga aaacttgacc cctgaggcgc tggaatctca ggccagaagc tatctgcaaa
      301 gtcaaatcac ctcaaccgca caatcttata ttgaagacac actttctccc tacggtaagg
      361 tccgttcgaa cctctccatt ggcaaggacg gcgatctgga tggcagctcc atcgattatt
      421 ttgttccctg gtacgataat caaaccactg tttatttcag tcaattttct gcgcagcgaa
      481 aagaagatcg tacaatcggg aatattggtc ttggggtaag atataatttt gatgaatggt
      541 tattgggcgg aaatatattt tatgattatg attttacccg aggacatcgc cgtttaggtt
      601 taggcggcga agcgtggact gattatttaa aattctctgg caactattat catccgcttt
      661 ctgactggaa agactctgaa gatttcgact tttatgaaga acgccctgcg cgcggctggg
      721 atattcgtgc cgaagcctgg ttaccttctt atccgcaact ggggggcaaa gttgtcttcg
      781 agcaatatta cggcgatgaa gtcgcccttt ttggtacgga taatttggag aaagatccct
      841 acgcggtaac acttggactg aattatcaac cggtgccatt actaaccgtt ggggcggact
      901 ataaagcggg gaccggagat aacagcgatg tcagcattaa tgccactctt aattatcagt
      961 tcggcgttcc gctaaaagat caattggata gcgataaagt gaaagcggct cactcgttga
     1021 tgggcagtcg tcatgatttt gttgagcgta ataactttat tgttctggaa tacaaagaaa
     1081 aagatccgct tgatgtcacc ctgtggttaa aagcggatgc caccaacgag caccctgagt
     1141 gcgtcattaa ggacactccc gaagcggccg tcgggctgga aaaatgtaaa tggaccatta
     1201 acgcgcttat caaccaccac tacaaaattg tcgctgcgtc atggcaggcg aaaaacaatg
     1261 ctgcccgcac gttggttatg ccggttatca aagagaatac cctgacagag gggaacaata
     1321 accactggaa cctggtgctg cccgcctggc agtacagctc tgacaaagcc gaacaagaaa
     1381 aactcaatac ctggcgagta cgtctggcac tggaagatga aaagggcaac cgacagaact
     1441 ctggcgtggt ggaaatcacc gttcagcagg accgtaaaat agagttgatt gttaataaca
     1501 tcgcgaacgt gccggaagag aacaaccaca gccacgaagc cagcgctcag gcagatggcg
     1561 ttgatggtgt ggtgatggat ctcgatgtaa ccgacagctt tggcgataac accgaccgca
     1621 atggcgatgc gctgccggaa gataacctca cgccgcagct ttacgacgcg caggacaaac
     1681 gagtgacgtt aaccaacaag ccttgctcga ccgacaaccc ctgcgttttt atcgctaaac
     1741 aagataaaga aaagggcact gtcaccctct ccagtacctt acccggcacc tatcgctgga
     1801 aagcaaaagc tgcgccctac gacgacagta actatgtgga tgtcactttc ctcggggcag
     1861 aaattggtgg gctaaatgca tttatctatc gtgtgggggc agctaaaccc agcaacctga
     1921 taggtaaaga taaagaaccg ctgccgctca acaacactta tcgatttgtt ttgtggcgtg
     1981 acagcaataa agacggtgtc ttccagcagg tcgaaaaact gactgacgaa gagatggcgc
     2041 agtacgacta caagtgggaa tttaccggca agagcatcaa tggtgaagtg ggtgcacagg
     2101 cgaacacttc caatgaggac attgtgattc cggccactaa ccgcgaagca gcgcaaacct
     2161 atggcgcaca agcgggagat ggcttgcagg gatacggttt acgcgtgctg tataccaaaa
     2221 aataagattg gcgtaaggcc agtcggccag tcgctgaagg ttcattaagg agagcatcaa
     2281 tgaagcagga taaaagacgc ggtctgaccc ggatcgcatt ggcgctggca ctggcaggtt
     2341 attgtgtggc acctgtggcg ctggcggaag aaagcgcctg ggtcgacagc ggcgaaacca
     2401 atattttcca gggaaccatt ccgtggctct attcggaagg gggaagcgct acgacagatg
     2461 ccgaccatgt aacgttgact tctgatctaa aaggcgctcg cccgacaggc agtgaaacgg
     2521 acaagcgtct ttactcgggt gataaattaa ccgtaagttg ggaaattggt gataccgaag
     2581 gcgacgtgga tcttggggga ttgggcgata acgcaaagac tatcgatact atccgctgga
     2641 tgagctacaa ggacgctcag gggggcgatc cgaaagagct ggcaacgaag gtgaccagtt
     2701 acactcttac cgatgacgac cgtggtcgtt atatcggtat tgaaattacg ccgaccacgc
     2761 agaccggtac gccaaacgtc gggactgcgc tgcatcttta tgacatttcc actgccagcg
     2821 gcggcggaag cgacagcgat aacgttgcgc cagggccggt ggttaaccag aacctgaaag
     2881 tcgccatctt tgttgatggt accagtatca accttatcaa cggcagcacg ccaatcgaac
     2941 ttggcaaaac ctacgtggcc aaactgtact cggatgagaa caaaaacggc aagtttgatg
     3001 cgggtaccga tgctgacgtt accgccaatt atgacttccg ttgggtactt tctggcagca
     3061 gccaacagct cggcacctcg ggtggtatcg ttaactcaag cttcgataat aacaatctgg
     3121 tcatccctgc gaccaacgaa gacgccagaa ccaaccttaa cggccctgcg cgcgatggta
     3181 aagaagctct taccatcccg accaacggcg acggggtaca ggggtacaaa cttcacatta
     3241 tttacaaaca caaataacgc gtactcagca gcattcccgc aagggaatgc tgtttccgca
     3301 tattttgctt aatcccggat taagcaaaat atgttgatgc gacaagcgtc cgtttaacaa
     3361 ccggtggagt ttctgtcatg tccctgaccc tgaataaaat ccttgtgtta tgcgcattgc
     3421 tgataagcgc aatgctgcct gggtggtcat gggcagaatc tgcatggcaa gactcttcag
     3481 atacggtcgg cgaatttaac ggtaccgtgc ccaccgccga cagtgccagc atacccgtct
     3541 accagggcag tgtctttctg gatcctgcaa agacgcatga ggtagcgttt accgctaaac
     3601 cgagtgaatt cagcgcggat attagcgtct ctaaattgct cgtcaccaat ccgcaagaca
     3661 gagaaggcga tatcatttcc accccacgtt gggaaaacca aacgccaccg acagtatctt
     3721 tagtttgggc ggatgccgcg acgccggaca ccttactgga cccacagcca gtcgcagatc
     3781 gctcgttttg cgctcaggga cttgccgggc gttcgctggt cgcctgggcg cagcctgatc
     3841 cccaacagac catgccgctg ttatatttac tcacctcgac gggctatccc tatgaaagtg
     3901 ttctgacgct cgccgatcag aaagtgacgc tgaagattgc gcccgcgcag ggtgacctta
     3961 tttccgtcag cgccgcgggc tatgacgaat cctccggtgc ggcgaaaacg acggttggcg
     4021 gcagtatcac gttaacggtc accaccaaag attgcgtcgg taatgttgtg gggaatattc
     4081 cttttgttat caaacgcaaa gatgctgaaa accgccaggg tgtggtgaac aatactgcgc
     4141 cggtaaaact gggcacgacc gaattaacca ccaccgcgac ggaatatcgt ggtactaccg
     4201 atgcgaatgg tgttgccacg gttacggtca cccaggccaa cggccctggt gtaaaaacgc
     4261 cgttggtcgc gagcctcgcg ggtattgctc aggccagtga aacggcagtg atctttactg
     4321 tcctcaccag cccggatgtc ccgcaggcta caatgtgggg tcatatgccc gatacgctga
     4381 aggcccgtga ttacactttt agtcgaccaa aactggcggc ggaagtcgat aatgaagacg
     4441 gtacggttaa cgatcacaat gaaacctggt ccacctttac ctggagcggc gcggataaac
     4501 attgcgacat tttgcccgga atgcgtcatt tcggtgcgct ggcgacggtg gtgccaacgt
     4561 cagtgcagga tgtggctggt tggccgatgc agggtaattt ctactggtca tcactggcgg
     4621 ggatgagcgg ccagcaccat gcggcggatg tttcgaaccg cagcgaagcg caaaaaccgg
     4681 acgatacgac gtttattgtg agctgtgtcg ataaagaagc gcccgatgtt gaaccgaagc
     4741 tggtactgac gccgggcagt tatgattcga cgataaaagc gatgaaagta aaggtggggg
     4801 aagaggcttc tttacgcctg acgattaccg atagcaagaa caacgatcag ccgctggctt
     4861 actactactt ttcgctgcat ctggatgacg gtgttaaccg taagaatcaa actgacgctg
     4921 cgtgggagac gcacccggta caaattgatg gtggcagtaa cgttcgcaaa gttgacgccc
     4981 atacctatga agggatcact gatgctaacg gagaggcctc tctgacgctg acccagccag
     5041 gcggcgtggg ggtgaaaaca cacatcaccg cgaaaatgcg cagtgatttt accgcctcgg
     5101 atgagaaaga cgttattttt acggtgatca ccagccctga ttccgacaaa gcgcggatgt
     5161 ggggacatat gttaggcatt attgaagcga ataatatctt taaacgccca cggctggcgg
     5221 atgaaaccgc caacgaactg ggagcagtgc gggaaaataa cgaagactgg gcgctgtttg
     5281 atcagaatag cagtatgcag gctgagtgtg gtttaggtca tattccgagc caaagctcat
     5341 tgcacagttt gtttgccgca catccagcca atgtcatcgg caccgaatat ggctggccaa
     5401 cgttgcaaaa agcgtatctt agtgcggtgg aagagacgtc gcacgcgtcg gtgaacctgg
     5461 ccacgggcaa catcgacacc tattccggct ttaagcaaaa ctacctttct tgttcgggca
     5521 atgagatggt cgcccagatt gctgcgacca ccgatcatga tgtttccaca agcagcagag
     5581 cgcaggcgaa agtgggtgaa acgattacca tgacggtacg cacctttaat gccttgaata
     5641 atgcgcccgt gccgtatacc gcgttcacca tcacgaaaga tatggggaaa aatcgtcagg
     5701 ggcagaccac gggtttcgat gatccaaccc gcggcgcaat cgagatgaat ggcacgctct
     5761 acggaacatc gcaaccgtct ttagtctatg caggtactac ggatgcgcag ggttttgcta
     5821 ctgtggaaat taagcaaccg caaggtgtgg gcttagcgac gccgctgaat attgcgcctg
     5881 taaactccta tattcccaat actgttaatt acaatgtgat tttcactacg ctcaccagtc
     5941 cggatgcggt tggcgcgcag atgtgggggc atatggatga gacgattacc gtcgatgcat
     6001 taacctttgc ccgaccccga ctggctgcgg aagtgtccag cccggacggc acattaacgg
     6061 aaaataacga agtatggtcg cgcgttagct aggccaacac ttccagcacc agtaaaggtg
     6121 gctgtggtgc caatatgctc ccacgccgtt cccagcttaa cgcgctctat gatgctaaca
     6181 gtggtaacgc cgttcaaacg gtacacggtt ggccaacgca gcgtcagccc tactggagca
     6241 gttcgcccgc ggatcaggtg ccgcattact acaccatagc cctcaacgat ggcgcgcgga
     6301 ccgttggtgg tagcacggcg gtttatgtca gttgcctgac gacggctaac aatcctgcca
     6361 gcagcattac gctggaagtg gtggacccgg cgcagtggaa cgcggcagca aatgcggcga
     6421 agctgaaaaa aggcgaaacg ctacaggtga aggtgacggt aaaagatgct cagggcaatc
     6481 cattgggtga tatgcctttt accctgaagc gtggcgatgg ttatacccgc tcaaatgaaa
     6541 aacacattgc cggtagcggt gatgcgctgg ttgcgccggt tgtgatcaat agtggcctgg
     6601 cggatgaagc ttcgctcaat gataccgccg cagcatacag cgcgatgacc ggcagcgatg
     6661 gcactaaaat tctcactatt acccgcccgg atacccatgg cacaaaaacg tcgctgaccg
     6721 ccgcgctcta ttcagacaca acgaaaaaag cgaccctcga taccattttt accgttgtca
     6781 ccagcccgga cagcaataaa gcgaaaatgt gggggcatat gccggaaacg gtgacggctg
     6841 cggatggaac ggtctttaaa cgcccattat tgttgaaaga gttatccagt acgtcgggtc
     6901 gaacagcgat tgcagaagat aatgaggact gggcgcaatt tacccaaact caggcgacaa
     6961 gcacatcctc taatggttgt ggtagtgaat atgttccctc tcaggccggg ctggagtcat
     7021 tgtatgaagc caaccgcggc aatgcgatga aaacggttca gggctggccc gtcgcctcaa
     7081 gttaccttac cagcacgaca ggcagcagta gtctggaaca gcgtgactta aaggccgtca
     7141 atctcagttc aggcaccagt tcgataattc catcggccac aaaagaactg cttacctgcc
     7201 agacgacgcc gatcgtcaag gctagccaaa tcgttcttga ggctgcggac cccacaaaat
     7261 ttgacagtac gaataatgtt gtcaaagcga aaaaagggga agaagtcgtg gttcgcgtga
     7321 ccactaaaga ttcgcagggt aacccggtgg gaaataccgc gtttacgctt aaacgtaacc
     7381 ttagcgtcaa tcgggcaaat gcgtctacaa cggtgacagc aggcgcgctc atcgtgacgg
     7441 atgcctgggg taacacgcag agtaattttt cctcaacaac agcactgatt tatggcgtta
     7501 ccggcgcaga tggtacgaca acacttgcac tgaaacagga caataccacg ggtttaaaaa
     7561 ccgaactgac cgccatgctg gatacagata ataacgtaaa atcaatgctt ccggtggtct
     7621 ttacggtgat aaccagcccc gataccccta aagcataatt ctgggggcat atggccgaaa
     7681 cgatgaccgg tgcaggcggg cttatttata aaagaccgtt attgcaagat gagctttccg
     7741 ttacaaccag tcgaagctct tttcaggaag atggagagag ctggtcactg ttctcgccgg
     7801 accagtcgaa caacacatct gtaaatggtt gcggtgcagg atatgtcccg attgaaagtg
     7861 agctggagtc actgtatgcc gatgaaggtt acgtgccaat acatgacgtt acgggatggc
     7921 ctgtatcacg cgcctatatc tccagtacgg ttgttgccta ttacactcaa acatttaatt
     7981 ttaaagcggt ttcgttaaaa acgggtgatg gaacggaggt catgtcaagc agtctaggac
     8041 tgctaagttg tcgggctacc ccagtggcgg tgaccagcca cattattgtt gaagcgaacg
     8101 atgcgacaca atttgtcaaa gttgacgaaa ccctcagcgc actaaaacgg aaaaaaggtg
     8161 acgacgcggt gattcgcgtg gtgacaaaag atgcgcaggg aaatgtcgta ccaaatgttc
     8221 cgtttattct caaacgtgaa ggatcgacaa atcgccagaa tgtacagttg tcgaacagaa
     8281 ctatcactgt aatcaacgct gcgggaacgt cggcaagagt ggatactcca tcaatctccc
     8341 tttatgcggt gacaggtgca gacgggacag ccacgtttac ggtaaagcag gatgattcta
     8401 taggcctggt aaccaatgtg tacgctcagg cctatcagtc gagcctggaa tccaacaaat
     8461 tacccgtgat gtttaccgtg ataaccagcc cggatacccc cctggcaagt tactgggggc
     8521 atatggcgga aacgttcact acgcgttctg gtactgcctt caaacgcccc ttactctctg
     8581 ctgaaagatc gtcgggccag tcgtttatag aagacaatga agagtgggcc gtgttgagat
     8641 cggcaacaaa gggggatatc gataaaagcg ggtgcgatgt acattatcaa ccactgttaa
     8701 gcgaactaca ggcattgtac gacgaacacc caagtcgtgc gataaagacc gatctgggta
     8761 ttccggtaaa tagctactgg tgggcttacg acatggtggc atatgcgggt aactggtacg
     8821 atcaatatat ttatctgtta aatggatcaa gtggaagagc gtcttcaacc acctcggcat
     8881 taatgctgtg cctggtaaac cctcatccag aggcggcttc tattgaaatg acctcaacgg
     8941 cggaggatgc aacgaaaacg gccagcaatg atggcaggcc cagcgcaaca gcgaagaagg
     9001 gagaggttat cccgatgact gttacggtgc gagatagcgc aggcaatcca ctgccaggtg
     9061 ccagctttaa ccttaaacga ggaactgcgc tgaaccgcgc taaagctgcg tacgatgcct
     9121 ctgcagatga tcttaccatc atacctgtgg aacctactgg ggtaacgtca atcttgtatg
     9181 gggatgggac gcaggctctt ctgaagacag ggagcgatgg taaagctacg tttgaggtca
     9241 gccagaatag cagttatggg ctgtcaacgc cgctcagcgc agaactcatg cgcgatacct
     9301 cgaaaagcgt cacgctggat gtcattttca cggtgataac cagcccggat tcgccaaaag
     9361 cgaaatactg gggccatatg ccggaaacgt ttaccagttc ggcaggcgtg accttcaagc
     9421 gcccattact ggcagcggaa gcgactacgg ggagctcggt aaacggtaat aatgagacat
     9481 ggtcatatat atatagtact caaaaagcga cggcagattg ttcgctggaa tatcaaccgc
     9541 gcctcagcga attgcagggg ttatatgacg atcacccaaa cggggcgtta acgaaagatc
     9601 tggggttgcc gatagcgtca ggaaactggt ggatttatga attacttaac tccaatggta
     9661 gctcctggta ttaccaggtc tttaacctaa gcacgggacg agcatcatct gcactctctc
     9721 cggttgctct aatgctctgc ctggcacagc ctcactctaa gccgtcaagc gttaccctga
     9781 cttcagtagc atttgatgag acgaaaaccg ccagtaacgg cggcacacct tcggcatcgg
     9841 cgaaaaaggg cgagacaatc ccgttagtcg tgacagtaaa agaccaaaat ggcaacctgc
     9901 tgagtggcga gagcgtgacg cttcagcggg cgcaggcgaa aagtcgttca ggaattaggc
     9961 cctcatcgtc ggcagatgat cttattgtag atgtcgtcac accgacagca acccgtattt
    10021 cctttgctca ggatacggca aaatggcttg gttttaccgg cagtgatggc acagtgacgt
    10081 tcaatgttac gcaaaacgcc accaccggcc tggcaacgcc gtttacggtc tcgcttgcac
    10141 aagatccgca gatcacgtca aacctggacc tgatatttac cgtggtcacc agccctgacg
    10201 cccaggatgc cgactactgg ggtcatatgc cagaaacggt gacagcggca aatggaaccg
    10261 tctttgaacg accgaaattg tggaaggagc tgccatcgac ctccggggta aataaaacca
    10321 ataacaataa tgaggactgg ccctattttt cgtcaaccac taaagcaaat gccagcctca
    10381 gcccttgtga ggaagcccgg caaccgttgc tcagcgatat gaagtcactg tatcaacgtt
    10441 atcccaataa cacccttacg acggaaactg gctggtcagt ttattattac tggtgggcgc
    10501 aggatcagtc gacagatggt aaaaatcagt cgctcaatat gaaagatggc acaacgacat
    10561 tgaatggtaa tgccgcttat caggcgtgtc tggtgtctgc gcgcgcaacg gtttccagcg
    10621 tcacactgac ctcaacagcg tttgacgcag ctattcaggc ggcgaaagtg aaaaaaggcg
    10681 aagccatgcc cgttaccgtg acggtgaaag acagcgccgg gaatacggta ccgaatgtgg
    10741 aatttacttt gaaacgtggc gatgcatcgc cgcgtaacgc tggggcgacg ttgtatggtg
    10801 atgtggtggc gatggacgat ctcatcgttc agccgttatc tggttcggct gttacgctga
    10861 gtgaaagcgg taataccatt agtggcatga caggcgcgga tgggacggca agttttacgc
    10921 tccgccagga taatacccct ggttataaaa cgccactgac ggttactctg gctaattacg
    10981 cttccgcgac agacacgctg gacgctattt ttacggtacc aaccagcccg aatgtttcct
    11041 cggcgcactt ttggggacat atggcagaca ctgtagtggt gaatggtaaa tcgctacatc
    11101 gaccgttgtt aacgacagaa ttgccgtccg gggctaatcc cgtatcctca ccgattatta
    11161 actacgaaaa ctgggcttct gcacatatca ttgatgccag taaatgggat attgcaaggc
    11221 agtgtggcag catagaaaat gcaccgacat acaatgaatt agagttgttg cacactgttt
    11281 ttaattcact gggatggccg tcttcaccaa gttttcctta tttgtcctct caacagtgtg
    11341 ggatggacga aggcactgga gcacaggatt gtagtatcac gctaatgaat aaaccgggtc
    11401 tggtgacctg cttccagtaa ccagcatggc gcatctggcg tggcctggga gccagtgccg
    11461 gatgcgatgc tggcgcatct tatggattag cagctttctt acgcggttta cgcgtttttg
    11521 ttacggcgct aacttcacta atcaccacac catcaccaag tcgagtggtc aacgtatcgc
    11581 ccgctttcac ctgcttcacc tgctttaaca ccgcgccatc tgcggcgctg gtgacgctat
    11641 aaccacgcgc cagcgtcgaa agcgggctaa ctgcttccag gtgcgttact gcattaccaa
    11701 accgttctcg cgttgcgctc agttgcgcgc gcaacgtttc tgccaggcga tattccagtt
    11761 gttgaattcg cgtctgtgcg cggtgaatac gcggctgcgg attttgctgg tttagcctct
    11821 gtgttaatcg ctgctgctgt tgcccggcac gcttaagctg attttccagc gcaaagctca
    11881 tccgcttttg caggcgctca agcatggttt gctggcgtgc cagccagagc tgtggatgct
    11941 gctgctgtaa gcgatgatgg atctgcgtaa aacgacgcgt gcggttggcg agataataat
    12001 ccattgccat ttcgaggcgc tgctgcgcag actgcacctg acgcagtaac tcctgttgat
    12061 tgcggctcac tacttcagcg gcggcagacg gcgttggcgc acgcaaatca gcaacaaaat
    12121 cggcaatcgt cacatcggtt tcatgcccga cggcacttac taccggaatg cggctggcaa
    12181 aaatcgcccg cgctacgcgt tcgtcgttaa aactccacaa atcttccagc gaaccgccgc
    12241 cgcgcccgac gattaacacg tcgcactcgt ttcgctgatt cgccagctca atggcgcgaa
    12301 caatttgccc tggcgcgtca tcgccctgaa cggcggtggg gtagatgatc accggcagag
    12361 aaggatcgcg acgttttaac acatgcaaaa tatcatgtag cgcagcaccg gtttttgagg
    12421 tgatcacacc gacgcaatgc gcaggggagg gaagtggttt tttgtattgc tgatcgaaca
    12481 gaccttctgc ctgcaatttc gctttaagct gttcgtattt ttgttgcagc aacccttcac
    12541 cggcaggctg catactttcg acgataatct ggtagtcacc gcgcggctcg tagagcgtaa
    12601 tattggcgcg gactaaaacc tgttgcccat gctgtgggcg gaaggtgacc cgtcgattgc
    12661 tgttgcggaa catcgcgcag cgcacctggg cggtatcgtc tttgagtgta aagtaccagt
    12721 gaccggaagc cggttgtgtg aaattagaga tttcgccgct gatccaaacc tgtcccatct
    12781 catgctcaag cagcagacga accgtttgat tcaggcgact aacggtaaaa attgcagggg
    12841 attgagaagg taacatgtga gcgagatcaa attctaaatc agcaggttat tcagtcgata
    12901 gtaacccgcc ctccggggat agcaagcatt ttttgcaaaa aggggtagat gcaatcggtt
    12961 acgctctgta taatgccgcg gcaatattta ttaaccactc tggtcgagat attgcccatg
    13021 ctacgtatcg ctaaagaagc tctgacgttt gacgacgttc tcctcgttcc tgctcattct
    13081 accgttctgc cgaatactgc tgacctcagc acccagctga cgaaaactat tcgtctgaat
    13141 atccctatgc tttccgcagc aatggatacc gtaacggaag cgcgcctggc tattgctctg
    13201 gctcaggaag gcggtatcgg ctttatccac aaaaatatgt ccattgaacg ccaggcggaa
    13261 gaagttcgcc gtgtgaaaaa acacgaatct ggcgtggtga ctgacccgca gactgttctg
    13321 ccaaccacga cgctgcgcga agtgaaagaa ctgaccgagc gtaacggctt tgcgggctac
    13381 ccggtcgtta ccgaagaaaa cgaactggtg ggtattatca ccggtcgtga cgtacgtttt
    13441 gttaccgacc tgagccagcc ggttagcgtt tacatgacgc cgaaagaacg tctggtcacc
    13501 gtgcgtgaag gtgaagcccg tgaagttgtg ctggcaaaaa tgcacgaaaa acgcgttgaa
    13561 aaagcgctgg tggttgatga cgaattccac ctgatcggca tgatcaccgt gaaagacttc
    13621 cagaaagcgg aacgtaaacc gaacgcctgt aaagacgagc aaggccgtct gcgtgttggt
    13681 gcagcggttg gcgcgggtgc gggtaacgaa gagcgtgttg acgcgctggt tgccgcaggc
    13741 gttgacgttc tgctgatcga ctcctctcac ggtcactcag aaggtgtact gcaacgtatc
    13801 cgtgaaaccc gtgctaaata tccggatctg caaatcatcg gcggcaacgt ggcaacagct
    13861 gcaggtgcac gcgctctggc agaagctggt tgcagtgcgg ttaaagtcgg cattggccct
    13921 ggctctatct gtacgactcg tatcgtgact ggcgtaggtg ttccgcagat caccgccgtt
    13981 gctgacgccg tagaagccct ggaaggcacc ggtattccgg ttatcgctga cggtggtatc
    14041 cgtttctcag gcgatatcgc caaagctatc gccgcaggtg caagcgcggt aatggtgggt
    14101 tccatgctgg cgggtactga agaatctccg ggtgaaatcg aactctacca gggccgttct
    14161 tacaaatctt accgtgggat gggctctctg ggtgcgatgt ccaaaggttc ttctgaccgt
    14221 tacttccaga gtgacaacgc tgccgacaaa ctggtgccgg aaggtatcga aggtcgcgtg
    14281 gcctataaag gtcgcctgaa agagatcatt caccagcaga tgggcggcct gcgctcctgt
    14341 atgggcctga ccggctgtgg tactatcgac gaactgcgta ctaaagcgga gtttgtacgt
    14401 atcagcggtg cgggcattca ggaaagccac gttcacgacg tgaccattac taaagagtcc
    14461 ccgaactacc gtctgggctc ctgattctct tcgcccgact tcatgtcggg cgatttatat
    14521 tatctgtttc acttgcctcg gaataagcgt caatgacgga aaacattcat aagcatcgca
    14581 tcctcattct ggacttcggt tctcagtaca ctcaactggt tgcgcgccgc gtgcgtgaac
    14641 tgggtgttta ctgcgaactg tgggcgtggg atgtgacaga agcacaaatt cgtgacttca
    14701 acccaagcgg cattattctt tccggcggcc cggaaagcac caccgaagaa aacagcccgc
    14761 gcgcgccgca gtatgtcttt gaagcaggcg taccggtatt cggcgtttgc tatggcatgc
    14821 agaccatggc aatgcagttg ggcggtcacg ttgaagcctc taacgaacgt gaatttggct
    14881 acgcgcaggt tgaagtcgta aacgacagcg cactggttcg cggtatcgaa gatgcgctga
    14941 ccgcagacgg taaaccgctg ctcgatgtct ggatgagcca cggcgataaa gttaccgcta
    15001 tcccgtccga cttcgtcacc gtagccagca ccgaaagctg cccgtttgcc attatggcta
    15061 acgaagaaaa acgcttctat ggcgtacagt tccacccgga agtgactcac acccgccagg
    15121 gtatgcgcat gctggagcgt tttgtgcgtg atatctgcca gtgtgaagcc ctgtggacgc
    15181 cagcgaaaat tatcgacgac gccgtagctc gcatccgcga gcaggtaggc gacgataaag
    15241 tcatcctcgg cctctctggt ggtgtggatt cctccgtaac cgcaatgctg ctgcaccgcg
    15301 ctatcggtaa aaacctgact tgcgtattcg tcgacaacgg cctgctgcgt ctcaacgaag
    15361 cagagcaggt tctggatatg tttggcgatc actttggtct gaacattgtt cacgttccgg
    15421 cagaagctcg cttcctgtca gcgttggccg gcgaaaacga tccggaagca aaacgtaaaa
    15481 tcatcgggcg cgttttcgtt gaagtattcg atgaagaagc actgaaactg gaagacgtga
    15541 agtggctggc gcagggcact atctaccctg acgttattga atctgccgca tctgcaaccg
    15601 gtaaagcaca cgtcatcaaa tctcaccaca acgtgggcgg cctgccgaaa gagatgaaga
    15661 tggggctggt tgaaccgctg aaagagctgt tcaaagacga agtgcgtaag attggtctgg
    15721 agctgggcct gccgtacgac atgctgtacc gtcacccgtt cccgggacca ggcctcggcg
    15781 ttcgtgtact gggcgaagtg aagaaagagt actgcgacct gctgcgccgt gctgacgcca
    15841 tctttattga agaactgcgt aaagcggacc tgtacgacaa agtcagccag gcgttcaccg
    15901 tcttcctgcc ggtacgttcc gttggtgtaa tgggcgatgg tcgtaagtat gactgggttg
    15961 tttctctgcg tgctgtcgaa accatcgact ttatgaccgc acactgggcg cacctgccgt
    16021 atgacttcct cggtcgcgtt tccaaccgca ttatcaatga agtgaacggt atttcccgcg
    16081 tggtgtatga catcagcggc aagccaccag cgactattga gtgggaatga tttgaccctg
    16141 cactatgaat gaacaaaacc ctctgttact acagagg
//
LOCUS       DAYPHN010000110        15986 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000110 DAYPHN010000000
VERSION     DAYPHN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.110"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(81..935)
                     /gene="gatY"
                     /locus_tag="ACG0BW_003284"
     CDS             complement(81..935)
                     /gene="gatY"
                     /locus_tag="ACG0BW_003284"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFK7260209.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(1163..2215)
                     /gene="fbaB"
                     /locus_tag="ACG0BW_003285"
     CDS             complement(1163..2215)
                     /gene="fbaB"
                     /locus_tag="ACG0BW_003285"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFK7260210.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            2472..3749
                     /locus_tag="ACG0BW_003286"
     CDS             2472..3749
                     /locus_tag="ACG0BW_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFK7260211.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            3746..4750
                     /locus_tag="ACG0BW_003287"
     CDS             3746..4750
                     /locus_tag="ACG0BW_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFK7260212.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGIESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKTELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            4747..5712
                     /locus_tag="ACG0BW_003288"
     CDS             4747..5712
                     /locus_tag="ACG0BW_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFK7260213.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAYPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(5686..6432)
                     /locus_tag="ACG0BW_003289"
     CDS             complement(5686..6432)
                     /locus_tag="ACG0BW_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFK7260214.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(6484..7302)
                     /locus_tag="ACG0BW_003290"
     CDS             complement(6484..7302)
                     /locus_tag="ACG0BW_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFK7260215.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFAHGDLPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHSNLAGYFNEYPWWVAH
                     YYQRRPDNDGIAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(7367..8167)
                     /gene="thiD"
                     /locus_tag="ACG0BW_003291"
     CDS             complement(7367..8167)
                     /gene="thiD"
                     /locus_tag="ACG0BW_003291"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFK7260216.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(8164..8952)
                     /gene="thiM"
                     /locus_tag="ACG0BW_003292"
     CDS             complement(8164..8952)
                     /gene="thiM"
                     /locus_tag="ACG0BW_003292"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFK7260217.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKRSQTPWTLDPVA
                     VGALDYRRCFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTPEVQA"
     regulatory      complement(8987..9083)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9175..9447)
                     /gene="rcnR"
                     /locus_tag="ACG0BW_003293"
     CDS             complement(9175..9447)
                     /gene="rcnR"
                     /locus_tag="ACG0BW_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFK7260218.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            9568..10392
                     /gene="rcnA"
                     /locus_tag="ACG0BW_003294"
     CDS             9568..10392
                     /gene="rcnA"
                     /locus_tag="ACG0BW_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFK7260219.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKDTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            10611..10949
                     /gene="rcnB"
                     /locus_tag="ACG0BW_003295"
     CDS             10611..10949
                     /gene="rcnB"
                     /locus_tag="ACG0BW_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFK7260220.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLINDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(11031..12065)
                     /locus_tag="ACG0BW_003296"
     CDS             complement(11031..12065)
                     /locus_tag="ACG0BW_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFK7260221.1"
                     /translation="MKIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALSQTQ
                     IATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNFSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLFGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPAKGN
                     KATINSNKGDIVLRCKNLLGQQNQTSRKMQVYLSSSDLWGSSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTISIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(12081..14561)
                     /locus_tag="ACG0BW_003297"
     CDS             complement(12081..14561)
                     /locus_tag="ACG0BW_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFK7260222.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTSW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(14577..15251)
                     /locus_tag="ACG0BW_003298"
     CDS             complement(14577..15251)
                     /locus_tag="ACG0BW_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFK7260223.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGENSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(15332..15874)
                     /locus_tag="ACG0BW_003299"
     CDS             complement(15332..15874)
                     /locus_tag="ACG0BW_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7260224.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         3944 a         4025 c         3727 g         4290 t
ORIGIN      
        1 aacatatgcc aatatgttca ccagctttat gccgggcaat taacgttttc atgttttttc
       61 cttgttaaat ggcaggtgcg ttatgccctg ccctcgcagc cacaatcggc aatcactttg
      121 ctcaccacat cgcgcattgc ggatttagcc gactgcaaat aatcccgggg atcggtcgct
      181 tcggggtgct cggtcaggta attttttaac gcctgcgaga aggcattttt cagctccgtt
      241 gcaacgttga ttttgcatat ccccagtttg atggtttgct gaatatcctt agtcgataac
      301 cctgacgcgc catgcagcac cagcggtaag ttcacccact ggcgaatgtt ctccagtcga
      361 gaaaaatcaa gcaccggtgc gctggcatac ataccatgag ccgtgccgat ggcgaccgcc
      421 agggaatcaa tcccggttgc ctcggcaaat tcacgcgcct gaacagggtt ggtgtacaac
      481 gcatcggctt cattgacttg cacatcatct tcctggccgc caagttgccc cagctccgct
      541 tcgacgctga catcaaagcg atggcaaaaa tccaccacct ctttgacccg tgaaatattt
      601 tgcgcaaaag gcaaatgcga ggcgtcaatc atgactgagc gcacgccaga acgaaccttc
      661 tgagcgatat cgtcaaattt cgtgtgatgg tcgagatgaa ttgccagtgg atggtgatat
      721 tgcttcgcca tcgcgctgac cagcgccaac agattttctg taccagcatg agtaaatgtg
      781 ccaggcgttc cggcgatgat gaccggtgca tgcaggttgg cagcggtttc taccaccact
      841 tgcatcgttt cgagattgtg aatattgaat gccggaaccg cataaccgcc gcgctgtgcg
      901 ttgttcagca tctgctttgt cgataccacg tacattttca tatcctgtcg tttactttcg
      961 atttcaaaac ataatgaaat gatttgtttt caaatgtcga gataatgatc acaaaaacga
     1021 caattcgaaa attaatcaga atgacaggca aaaaaacggc ctcccgatag ggaagccgta
     1081 gcaaagtgcg cgtgttttta tgccggatgc gacgtaaacg ccttttccgt catacgtggt
     1141 agtgctgttc gtataaggcg tatcaggcaa tagtaatttt gctatcgaga taaacgtcct
     1201 gcacggcgtt aatcagtttc acgccgtcag ccatcgattt cttgaacgcc ttacgtccaa
     1261 gaatcagccc cattccgcct gcgcgtttgt tgataaccgc agtacgcact gcatcgctga
     1321 ggtcagtttc accgcctgca gcaccgccgg agtttatcaa ccctgcgcgg cccatatagc
     1381 agttagctaa ctgataacgc accagatcaa tcgggttttc gctggtcaac ttgctgtaca
     1441 cgcgatcgtc ggtataaccg taattaatcg ctttatagcc gccgttattt tccgccattt
     1501 tttgtttaac gatatctgca cctatggtcg ccgccagatg gtttgcctga ccggtcaggt
     1561 cggcggaaac atggtaatca acgccatctt tcttaaaggc ggagttacgc aaataggccc
     1621 acagcactgt caccatgccc agctcgtgcg cacgttcaaa agccgcagaa atttcttcaa
     1681 tctggcgacg tgactcttcc gaaccaaaat agatagtcgc accaaccgcc accgcgccca
     1741 tgttgaaggc ctgctccacg ctggcataca gcgtttgatc gtaggtattc gggtaactta
     1801 gcgtctcgtt gtgattaagt ttgacgagga atggaatgcg atgcgcgtaa cgccgcgata
     1861 ccgatgccag cacgccgtaa gttgacgcca cacagttaca gcccgcttcg atcgccagtt
     1921 caacaatgtt tttcgggtca aagtagagcg ggttagcggc aaatgaagct ccggcagagt
     1981 gctcaacgcc ctggtcaacc ggcagaatag aaagatatcc tgtgccagcc agacgcccgg
     2041 tgttgtacaa cgtctgcata ttacgtaaca ccgctggcgg gcgattattg tcaatcatca
     2101 cgcggtctac gtagtcatgt ccggggagat aaagctggtc agaaggaata gtcatacaac
     2161 ggtgctgtaa aaggttgtcg gcgtctttgc caagcaactg cgcaatatct gtcattacta
     2221 tgctcccgta aattccgatt ggatatcggc tatggattgt cctggcccgc cttttgcggg
     2281 caatcataat cctggtcgtt acaggcgagt ttttccatct tttggatgtt ttatcagcgt
     2341 tttctgttgg ctcgattcat cagaaaaaat gttaccgcgg tcaagttttc accgcaaagg
     2401 tatttaaaag gtattattaa atggtattgt catcgcgtac cttacattac ctgtcatgaa
     2461 ggaattaaaa gatgaaaaca acagcaaagc tgtcgttcat gatgtttgtt gaatggttta
     2521 tctggggcgc gtggtttgtg ccattgtggt tgtggttaag taaaagcggt tttagtgccg
     2581 gagaaattgg ctggtcgtat gcctgtaccg ccattgcggc gatcctgtcg ccaattctgg
     2641 ttggctccat cactgaccgc tttttctcgg cgcagaaagt gctggcggta ttgatgttcg
     2701 ctggtgcggc gctgatgtat ttcgctgcgc aacagaccac ttttgccggg ttcttcccgt
     2761 tactgctggc ctactcgcta acctatatgc cgaccattgc gctgactaac agtatcgctt
     2821 ttgccaacgt gccggatgtg gagcgtgatt tcccacgcat tcgtgtgatg ggcactatcg
     2881 gctggattgc ctctggtctg gcatgtggat ttttgccgca aatgctgggt tatgccgata
     2941 tctctccgac taacatcccg ctgctgatta ctgccggaag ttctgctctg ctcggtgtgt
     3001 ttgcgttttt cctgcccgac acgccgccaa aaagcaccgg caaaatggac attaaagtca
     3061 tgctcggcct ggatgcgctg atcctgctgc gcgataaaaa cttcctcgtc tttttcttct
     3121 gttcattcct gtttgcgatg ccactggcgt tctattacat ctttgccaac ggttatctga
     3181 ccgaagtcgg tatgaaaaac gccactggct ggatgacgct cggccagttc tctgaaatct
     3241 tctttatgct ggcattgccg tttttcacca aacgctttgg tatcaaaaag gtattattac
     3301 ttggtctggt caccgctgcg atccgctatg gcttctttat ttacggtagt gcggatgaat
     3361 atttcaccta cgcgttactg ttcctcggta ttttgcttca cggcgtaagt tacgattttt
     3421 actacgttac cgcttacatc tatgtcgata aaaaagcccc cgtgcatatg cgtaccgccg
     3481 cgcaggggct gatcacgctc tgctgccagg gcttcggcag tttgctcggc tatcgtcttg
     3541 gcggtgtgat gatggaaaag atgttcgctt atcaggaacc ggtaaacgga ctgactttca
     3601 actggtccgg gatgtggact tttggcgcgg tgatgattgc cattatcgcc gtgctgttca
     3661 tgattttttt ccgcgaatcc gacaacgaaa ttacggctat caaggtcgat gatcgcgata
     3721 ttgcgttgac acaaggggaa gttaaatgaa aacagaacgt attctcggtg ctctttatgg
     3781 gcaggcgtta ggggatgcga tggggatgcc ctccgagctt tggccacgca gccgcgttaa
     3841 agcacacttt ggctggattg atcgttttct gcctggacca aaggagaata acgcagcctg
     3901 ttattttaac cgcgccgaat tcaccgacga tacctcgatg gcgctgtgtc tggcggatgc
     3961 gttactggaa cgtgaaggca agatcgatcc agatctgatt gggcgtaata ttctcgactg
     4021 ggcgctgcgt ttcgacgcct ttaacaaaaa cgtactaggt ccgacctcga agattgcgct
     4081 taacgccatt cgcgacggta aacccgttgc tgaactggaa aataacggcg tgaccaacgg
     4141 cgcagcgatg cgcgtctcgc cattaggttg tttgcttcca gcgcgtgatg ttgattcctt
     4201 tattgatgat gtggcgctgg cctccagccc gacacataaa tccgatctgg cggttgcggg
     4261 ggcggtagtc atcgcatggg cgatttctcg tgccattgac ggagaaagct ggtcagcaat
     4321 tgttgattca ctgccttcaa ttgcgcgaca tgctcaacaa aaacgcatca ctaccttcag
     4381 cgcctcactg gcagcacgtc tggagattgc gctgaaaatt gtgcgcaatg ccgacggcat
     4441 cgaatccgcc agcgaacagc tttaccaggt cgttggcgca ggtaccagca ctattgagtc
     4501 cgttccgtgc gccattgcgc tggttgaact ggcacaaacc gacccgaacc gttgcgccgt
     4561 cctgtgcgct aaccttggcg gcgacacaga caccatcggt gctatggcga cggcaatttg
     4621 cggcgcattg catggcgtta acgcaatcga tcctgcgtta aagacggaac tggatgcggt
     4681 aaatcagctt gatttcaacc gctatgccac ggcgctggcg aagtatcgcc aacaacggga
     4741 ggcgatatga gcggcgctcg attacacacg ctgctgcctg aattagccac gcgtcagccg
     4801 gtgatggtcg tcggcgcggc ggtcattgat gtgatcgccg acgcttatgc cctcccctgg
     4861 cgtgggtgcg atatcgaact gaaacagcag agcgttaacg ttggcggctg cgcactgaat
     4921 attgccgtgg cgttaaagcg cctcggcatc gaagcgggta atgccttgcc gctcggtcag
     4981 ggcgtgtggg cggagattat tcgcaaccgg atggcgaaag aggggttaat cagcctgatc
     5041 gataacgccg aaggcgataa cggctggtgt ctggcgctgg ttgagccgga tggcgaacgc
     5101 acttttatgt cattcagcgg tgtggaaaat caatggaatc gccaatggtt ggcacgatta
     5161 accgttgcgc ctggcagcct gctctatttt tccggttatc aactggcctc gccctgcggc
     5221 gaattgttag tggaatggct ggaagagctg caagacgtga cgccgtttat cgattttggc
     5281 ccacgtattg gcgatatccc ggacgcatta ctggcgcgga tcatggcctg tcgaccttta
     5341 gtgtcactca atcgtcagga ggctgagatt gccgccgaac gttttgcttt atccgcagag
     5401 ataacaacac ttggcaagca atggcaggag aaatttgccg cgccgttgat cgttcgtctc
     5461 gataaagaag gcgcatggta tttcagcaac gacgcttctg gctgcattcc ggcatatcca
     5521 acgcaagttg tagacaccat tggggcgggc gacagtcatg caggtggcgt acttgccggg
     5581 ctggcctctg gtctgccact ggcggatgct gtattactgg gcaatgcagt ggcgtcgtgg
     5641 gttgtcgggc atcggggcgg tgattgtgcg ccaacgcgcg aggaactact cctcgcacac
     5701 aaaaacgtat agatcgctgc gacagtggct gatgctgtac tcaataggcc gttgctgttg
     5761 gtcaagcgcc acttgcttga tcaccagcac cggtattttg ctatctaact gaatatgtga
     5821 ctgaaactcg gcatccggca tccgggcact aacgcgggaa cgcgtacgtt gtgggtaaat
     5881 gtgctggctg cggaagtagt cataaagaga aatcccgatg gcatcaacat catgaattaa
     5941 atgtgccgga acccacgatt cctcaatcga taccgcttct tcatcgacat agcgaatacg
     6001 cttaagcaaa aagacatcac ttcccgcttc gaccgccagc tgctgcgcga cttcttcggg
     6061 acatttcacg acacgcttgt taacccatag tgtatcgggc tttttcccgc gcaataccac
     6121 ctgctgagaa aaacctcgcg cttctttcaa cgagtattcg aagatgttgt tgatctgcgt
     6181 gccgtaacca cgcgaacgcg tcaccacgcc ctcttcttcc agcgcctgca tcgccttgcg
     6241 caccgtaatg cgcgaaacgc cggttaactg acttaaatca cgctcaccgg gcaaaatatt
     6301 gccatgctcc agcaccccgc tgcgcacggc attttttacc gtttcggcaa acttaatata
     6361 aagcggcgtg ttatccgccg ctaaaatacg ttcgttcagt tgggcaatca gctgggtatg
     6421 cgcttgttcc atttgtcttt ttcctgccag ggagtctggt gggagtatat cactgcttct
     6481 tatttaaggc gtttctttaa tcccatcaac gaatgcctgc aactcctcca ccgtgccatt
     6541 aaacacatta aaatccaccg gaccattaat gccatctacc tgtccacggt cggaatgctg
     6601 ccagaagcgc caggctattc cgtcattgtc cggacgacgt tgataatagt gagccaccca
     6661 ccacggatat tcattaaaat agcccgccag attcgagtga taaaaaacgg ctcctgagta
     6721 aataatcggc ttttttcccg tgcgtttttc gaccattttt agccactgac ttacccgctt
     6781 gcgtaattct tttgccgata attttccccg ttcttctacg tccagcacgg cagggagatc
     6841 gccgtgggca aaatccaccg tttgcagaaa taatcgcgcc tgaactgaag cggataccga
     6901 cggggaaaaa taatgatacg ccccgcgcag caggccattt tcgcggctta gttgccagtt
     6961 acgcgaaaaa taggggtcca ccagcttttc gccttccgtc gccttaataa aggcaaattg
     7021 taagcgaata ccgttatcgc gcatttttgc cacccgctgc cagtcgatcc gctcctgcca
     7081 gcgcgaaaca tctattccgt gaatggtgta actggcagga atgcgaatag cgaaagattt
     7141 aaccgggcga taaccgtaga agtggatgta gtctttaatc tgaattgctg tcgaatagaa
     7201 aaaattcacc gtctgacgcg gataaattgc aacgatggaa attagcccaa gagcgcataa
     7261 taaagcggtg aatttttttc gactggtgat tctcaactgc attggtttca tccctgaatg
     7321 tcagcaccaa aggctgacaa taaccaaagc ggctatagta cggtgctcac caccaggcgt
     7381 ggaagtggtg aaccggacca ataccgtgac caacttccag cgtgtcggcc tgggctaatg
     7441 ccgatgaaag ccagcttttt gcctcctgta cggtgtcagc ccagtttgta tggcgcgggc
     7501 gcagtgcagc caacgccgca gagagcgtac aaccagtgcc gtgagtgttt ttggtcataa
     7561 tgcgctgtgc ggtaaaccgt tgttcaccct cgcgggtaaa cagccagtcc gggctttgct
     7621 catcatccag atgaccacct ttcattagca ctgcgccaca gcccatcgcc aacagcgatc
     7681 gcccttgttc cagcatttcc tgttcggtgc gcgcgtgtgg cgcgtcgagc aaggcggctg
     7741 cttcgggcaa gtttggcgtt attaatgaaa cctgtggcaa taatcgactg cgtagcgtgg
     7801 caactgccga aggtgaaagc agcgggtcgc cgctttttgc cagcataacg gtgtcgagta
     7861 ccacgttttg gatctgataa cgttgcagcc gttctgccac cgcttcaaca atatcggttt
     7921 ccgccaacat accgatttta gtggtatcga ttcgcacatc gctgaacacc gaatcgagct
     7981 gggcggcgac aaaatcaggc tcaatgcgat aaaccgactg tacgccacgg gtattttgcg
     8041 ccaccagcgc ggtgattacc gagcaaccat acgcacctag cgccgagaag gttttaagat
     8101 cggcctgaat ccccgcacca ccactcggat cagtaccggc aatcgtcaga gcgttaattc
     8161 gtttcatgcc tgcacctccg gcgtcaattg ccagagcgca tcaaggaaat gtggaacaaa
     8221 actgcctggc ccctcgcttc tggcgactgc gcgttctccg gcttgtttca tccagtgaca
     8281 ggcagatgcg acgttttcca gcatatcgcc gggtaacgca caacaggcag cgacaaccgc
     8341 cgataatgca cagccagttc ctaccacttt ggtcattaac ggatcgccac cgtgaatgcc
     8401 aatgatacga tgtccatcgg taacataatc cacctcgcca gtgaccacga cgattgcgcc
     8461 agtttcccgt gccagtgttt gtgcagcggg tatcgcatta gctgcggcgt cagtggtatc
     8521 cactccccgt ccgccattag caatgccagc taatgccatg atttccgaag cattaccacg
     8581 tatggctgtt ggcttatgag acagaagttc cagacaaaaa cagcggcgat agtcgagtgc
     8641 accaaccgca accggatcaa gcgtccaggg tgtttgagag cgttttgctt gctcaacggc
     8701 ggcacgcatc gcctgtgcgc gtggctgcgt cagtgtgcca acgttaatca gcaacgcact
     8761 ggcgatagcc gcaaactgac tggcttcttc ggtttcgata accatcgctg gcgatgcacc
     8821 gagcgccagt aaggtattgg cagtaaaagt ttgcaccaca tcattggtca tgcaatgcac
     8881 aagaggggaa tgttggtgaa aaaggtgtaa cgcgtgcgca gattgcgcgg aactcagcag
     8941 gtcgacttgc atagtttgct cctgccataa cgtgaagaag caatgacctg gtggtccgtg
     9001 acttccctac gctggcatta tccagatcag gtgatacggg tatttctcag ccttcacgca
     9061 gaagggcacc ccgagtcgtt tgattgcgat gataaatcgc agaggaggat ggtaatgtcc
     9121 agcgcacgcg ttgtaaacga gtaaaaaccg cagttttaca atcgcgttaa taaattattt
     9181 gatataagaa tccagcacct tcagaacgac atccagatct tcttcacgtt ttagctcatc
     9241 cccctggtga acgatgtgtt ccgtcagatg acctttaatc acttcccgca tcagaccgtt
     9301 taccgcgcca cggatagcag caatctgttg taaaactgca gcgcattcgt gcggctcgtc
     9361 gagcattttc ttgagcgcca cgacctggcc ctgaatctta ctggcacgcg ctttcagttt
     9421 ctgtttatca cggatggtat gagacatggc aacacctggt taacaagaat atgaaaaatc
     9481 atagcactat taatctactg gggggtagta tcaggtactg ggggggagta gaataagatt
     9541 gccgaattaa tactaagaat tattatcatg accgaattta ccactcttct tcagcaagga
     9601 aacgcctggt tcttcatccc cagcgccatc ttacttggtg cgcttcatgg cctggaacca
     9661 gggcactcaa aaacgatgat ggcggcgttt atcatcgcca tcaaagacac cattaaacag
     9721 gcggtgatgc tcggactggc agcaaccatt tcgcataccg cggtggtctg gttgattgcc
     9781 tttggcggga tggtgatcag caagcgcttt actgctcaat cagcagaacc gtggctccag
     9841 ttgatttccg cagtgatcat tattggcacc gcgttctgga tgttctggcg tacctggcgc
     9901 ggcgaacgca actggctgga gaatatgcac gagcatgatc atgagcatca tcaccacgat
     9961 cacgaagatc accacgacca tggacatcat caccatcacg aacatggcga gtatcaggat
    10021 gcccatgcac gagcccatgc caatgacatt aaacgacgct ttgatggtag agaggtcacc
    10081 aactggcaaa ttttgctatt tggcttaacc ggtggcctta tcccctgccc ggcagcaatt
    10141 accgtgctgt tgatatgcat tcagttgaaa gccctgacac tgggcgcaac actggtcgtc
    10201 agtttcagca ttggcctggc gttaacgctt gtcaccgtag gcgttggcgc agcaatcagc
    10261 gttcagcagg tcgcaaaacg ctggagcgga tttaacactc tcgctaaacg cgccccctat
    10321 ttttccagtc tgttgattgg cttagttggt gtgtatatgg gcgtacatgg cttcatgggc
    10381 ataatgcgat aactcaatcc tttaggcttg catgttatct tcagcccagg atgattctta
    10441 aatcagctat ttccgctgac tctctgctcg ctaaggatgc ttttagggca tcctttcatt
    10501 tacacttttt acgaaatcat gggatcacta acaaaatatc gcttgtcagt tatattgtat
    10561 ggcaggaaag atatgcgact gatattacag atccccaaag tggagagttt atgaccatta
    10621 aaaataagat gttgctgggt gtgcttttgc tggttaccag tgccgcctgg gccgcaccag
    10681 ccaccgcggg ttcgaccaat acctcgggaa tttctaagta tgagttaagc agtttcattg
    10741 ctgactttaa gcatttcaaa ccaggcgata ccgtcccaga gatgtaccgc accgatgagt
    10801 acaacattaa gcagtggcag ttacgtaacc tgcccgcgcc tgatgccggg acacactgga
    10861 cctatatggg tggcgcgtac gtgttgatca acgacaccga cggtaaaatc attaaagcct
    10921 acgacggtga gattttttat catcgctgaa aaaaagcccc ctcgaagaga gggggaaatg
    10981 cagacacctt attattttta ttattagcca cttgctcgtc ttgcttgtta ttagtcgtat
    11041 ttcacgttga ttaatgcggt tgcctccagt gcgccagatt taactttgtt tgtatcgtag
    11101 acgtagtaac tggcagttat cggaattgaa atcgttgtat ctgtaagttt tgacttcgca
    11161 gcaacttcct ttaaatttgt atatccttta ctgctgttag tgatatttaa aagtgtgact
    11221 ggcgaaccat tactttcaag aataaatcct acgccattat cttccgcacc tttcaggatc
    11281 gtgttactac tgccccacaa gtcagaactt gataaataca cctgcatttt ccgtgatgtt
    11341 tgattttgtt gacctaataa gtttttacaa cgcagaacaa tatccccttt gttactattt
    11401 attgttgctt tgttaccttt ggctggtaat tgtgaaacgg gaatactctc taaagtaatt
    11461 aataaatcgt ccggtttgca ggttgtggtt atcgggttgt atgacaatgt cagattaaag
    11521 gaatagacac ctgcattttg tgccaatgca tttctgtagc tatttacaca tgcatcccat
    11581 gacttgccac cgaataaaca cagtaaaaat tgtactgcag cactcccgcg tgtcgatgat
    11641 gaagcactgg ataatgttgc aacaccatta atcattactg aaccgccgcc ccccgcagaa
    11701 aaattcactt ttgcattact ggccgcaggt gtcaccgtta ccgaataacc actggtgaaa
    11761 ctggtctgat tatttgacaa cgtgatgttg tttttattgg tccattcaaa atgtaaattt
    11821 aatttaatgg tatcgttata aagtccaaca ataatatccg acgggctcat aggggtgata
    11881 gtatttgtac tcacgcatga cgaatcatta aaattatgct catatgtcgc tatttgtgtt
    11941 tgactaagtg caaaatcccc ctgaacaacc aaattgtgct gaacagataa ctcactataa
    12001 catgctgcat agctcactgg cataaccatc atcattaata taaatagcat tatgcgaatt
    12061 ttcatagaac cacctgacta ttactggcaa atataatttc tactttcatc aatctcttta
    12121 ccgaaggtga ttgtgcatga aagtccttgt tgcttatcaa ttgctacatt aaccgatggc
    12181 ggaacttcat tggtgcgaat aaataactgg cttccctggc cgacaacgcc aatattatga
    12241 ccatggatat cattgacttc ataaccaaac gttaatggtt gcccatccgt tcttaacgct
    12301 tttataaacc agggcttgcg ctgatcggta tcaaaattaa ccagtacaac cgcgccgcga
    12361 taaggtgcgg caattttccg gttaccgcgc aattctgctt cgctatcgct ttgcgacaca
    12421 tccagcatca ggtgattttc ccgataaggt gtcattccgt cgtataccac cactccatta
    12481 cggtttgttg tgcgatattt ttgcccattg acataagcat ctttaattcc tggcgcattc
    12541 atcactgcaa acgtttcgga aagacggttc gccagattaa cgccacccga ccaggcgaca
    12601 atgccccctg aaacactggc tccagcctgt cgataagtac tcgactgact ataactgcca
    12661 ttcaccgttg ccaccggcgc gttccaggtt aaattcgctc cagctgttgt ttcatttccc
    12721 tgatgctgat gactcaggtt gaccccatag ttaaattgat cccggtttcc aacggttccc
    12781 gataatcccg tattatttga ggcaaaaccc tgatcatcaa acgtcgttga gttagacata
    12841 tatatttgcc gacgaggcgt cgtaacgtca tcaccccaat caaagggaat cgatataaaa
    12901 atattaaaac gtttctcttc atgatgattc tcgtcataag cctggcttgc cgcgagggta
    12961 tagcttatcc gtcgcaggtt gttggaataa ctcaactgat aatccttact gctgccgcta
    13021 cgcccccagt aatctcgcca taacgtactt aaggacacag aaccccagcc ttctggcaat
    13081 gactggctca tattggcaga aaagctattt ttacggccaa aatcgttctg gtaataatcg
    13141 gcaatgtcat agatatcgtt ttcatcacgg cgataattat ctttattgtt tgcccaaacg
    13201 tgatcgttaa atgtccggta atcacgcgac gaataacgcc aggccgccag accaaaacgc
    13261 gtcgacgttt ggctcacaaa tttgttgtag gcaatttgat aactttgccc gtcaaacaca
    13321 tcgccgttgt cttgtttact atgcgacttc gtggcatcga cggaaatggc accaatgcgt
    13381 gtattccagc tagtccccaa agtgaacgcg taataattat tcgcgaccat cgatccgcca
    13441 tacagcgtca ataagttatt aaaaccatac tgataacccg cctggacaaa atcactttgt
    13501 ttgctcgccc cttcaatatg gctacgaccc gccgcaaaat catatttcga cacgccaggt
    13561 tgcagcatat tcggcaccgc tgcataaggc accagatagg tggttaccga accgtccgcc
    13621 tctttcacgc tgacatcaag atctgctcca ccaccggcca actgcaaatc tgtaatcgca
    13681 aacgggccag gaggaacctc tttctgataa accacaaaac cgttctgttc aatagttacc
    13741 agcgcgttac tctgagcaat cccctgcacc cgtggagtaa aattctgttt cgagttaggc
    13801 aacatctgca tatcacgaaa caaccgcaca ccgctgaagc gaacagaatc aaaaatatcg
    13861 cttgatgtgt acatatcacc cacgcgaagc gtgccgagaa gttgggcaaa tccacgttcc
    13921 agatacaggg tattgctttt ccacacccct ggattgctat ttgttttact gaaactggca
    13981 tcagaatgca gttgccaccc cagtaagttt aacccgctgt taaaacgtac atatgtactc
    14041 ttgctattac ccgacgcttt atagtcgctg taatactgac tcacataata agaggtataa
    14101 aacgcattaa tacctcgctc ccagttttcc ggtggaacat agccactttc cagttcttcc
    14161 acccacgcct gcgggacact gaaatcgaga cgaaaaactc cgatatccca ggtatagctc
    14221 ccaccctgaa caagttgctc aaatgttaaa cattgcttac cgctggcaaa gttatcggta
    14281 ttaatgccta accgcttgat aatttccctt gataaacatg tttcttgcgg gttgtcttta
    14341 acaatgatct catatttccc gcgccattgc ttattgacat aaatatcgat gtcatactgc
    14401 cccggcaagg gttgattatc atcaagacga atatttgcaa cctgctggtc tttcattcca
    14461 cctatcataa aatgggtatc aaaggtttct tcagctgcat aagcttcaat gccgagcaat
    14521 aacgctacga ttgctgatgc aagtggggtc attctcaaca taggcagctc ctgcaattaa
    14581 attttgtcac taatatagtt gccatggtca tcgataatgg tcagatacca gttattcgca
    14641 ttattacttt tgacattaat actctgactt tctaaggggg caatcataat agtttcataa
    14701 ttgactttga cattattagc tttgacatcc gaaatcgtta cccaatttgc tgagtcattt
    14761 tttatcacca atccattgcc actgcgattt accagtaact ttttaaatgt cgctttattt
    14821 accggtgcaa tacccgcagg ccggtaaaac aatttgattc tgttttgcat tgcaaatttc
    14881 agtgcattct tgccctcttg ctctggacta tttggtggaa tatccagaac attcagataa
    14941 aaaatgctct ctttattagt gggcagttta ttcggcataa ttttgatttt tacttgctgc
    15001 ccggaatttt cccctatttt tgccactggt ggcgttaaca tgaaaggaac ctgaattttt
    15061 tctggtggta acgacgtatc accatcatca atccacgcct gcagcagcga agaacggttt
    15121 ccctggttca tcaactgcac catcacttct ttgtgctctg ccgggtaaat aatacgcgtc
    15181 ccgtagataa caataccggc atgtgcagga aggaccatag aagaaatgag taaagctaat
    15241 aatcctttca tcttaatgcc tcttaaatta aaaggccgcc atggggatag cggccatgtt
    15301 tattaactgg agaaacttga aattttattt attaatcctg cgccacacgc ataaccagag
    15361 aagataacac atcacctgat tgcactgaat tatctgtcag cttcaggtat tttgctttaa
    15421 acggaatagc atattgacct tccgctgttt tataactctc aatgttaaaa ggtgcagaaa
    15481 catccaggac atcgctgtta tcacctaaat agagatagta ccccacggta tcgttgctac
    15541 tggctatcgc cttatcgttt ccggttattt tatcgttggt aatcaacgag agttttacac
    15601 cagcaacttc tttcggacag ctaattttta acatgctgct ggttacaccg actgcatcat
    15661 cataggtcga gtatttagct ttaccttcaa agacagtggt tggtacagtt ggcatattaa
    15721 tacttactga atcgccaccc gcttcgaatt tacacggaga ttctgcaata ttccctttaa
    15781 tggttaatgt tccggtatca acgtctgcgg caaatacccc agcgctcata aaaaaagaag
    15841 aaaagacagc ggcagcaata attgaacgtt tcataatatg ttccttaata aaatttattt
    15901 ttggttgtta agagtctgtg tctcttggaa acaaatttct cacatcaaaa aagttgccgc
    15961 aatgtaaaca cattgtttta ttgatt
//
LOCUS       DAYPHN010000111        15890 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000111 DAYPHN010000000
VERSION     DAYPHN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.111"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1351)
                     /gene="uidB"
                     /locus_tag="ACG0BW_003300"
     CDS             complement(<1..1351)
                     /gene="uidB"
                     /locus_tag="ACG0BW_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFK7260225.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWAPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGSCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQAPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     "
     gene            complement(1348..3159)
                     /gene="uidA"
                     /locus_tag="ACG0BW_003301"
     CDS             complement(1348..3159)
                     /gene="uidA"
                     /locus_tag="ACG0BW_003301"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFK7260226.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGIKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3550..4140)
                     /gene="uidR"
                     /locus_tag="ACG0BW_003302"
     CDS             complement(3550..4140)
                     /gene="uidR"
                     /locus_tag="ACG0BW_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFK7260227.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4361..5128)
                     /gene="hdhA"
                     /locus_tag="ACG0BW_003303"
     CDS             complement(4361..5128)
                     /gene="hdhA"
                     /locus_tag="ACG0BW_003303"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFK7260228.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5240..6268)
                     /gene="malI"
                     /locus_tag="ACG0BW_003304"
     CDS             complement(5240..6268)
                     /gene="malI"
                     /locus_tag="ACG0BW_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFK7260229.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6443..8035
                     /gene="malX"
                     /locus_tag="ACG0BW_003305"
     CDS             6443..8035
                     /gene="malX"
                     /locus_tag="ACG0BW_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFK7260230.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMNTVQA"
     gene            8045..9217
                     /gene="malY"
                     /locus_tag="ACG0BW_003306"
     CDS             8045..9217
                     /gene="malY"
                     /locus_tag="ACG0BW_003306"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFK7260231.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNSALPELNWQIPQSTYLAWIDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9321..10322
                     /gene="add"
                     /locus_tag="ACG0BW_003307"
     CDS             9321..10322
                     /gene="add"
                     /locus_tag="ACG0BW_003307"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFK7260232.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10358..11398)
                     /gene="ydgJ"
                     /locus_tag="ACG0BW_003308"
     CDS             complement(10358..11398)
                     /gene="ydgJ"
                     /locus_tag="ACG0BW_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFK7260233.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11641..11766
                     /gene="blr"
                     /locus_tag="ACG0BW_003309"
     CDS             11641..11766
                     /gene="blr"
                     /locus_tag="ACG0BW_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFK7260234.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            12039..12254
                     /gene="ydgT"
                     /locus_tag="ACG0BW_003310"
     CDS             12039..12254
                     /gene="ydgT"
                     /locus_tag="ACG0BW_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFK7260235.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12340..12780
                     /gene="ydgK"
                     /locus_tag="ACG0BW_003311"
     CDS             12340..12780
                     /gene="ydgK"
                     /locus_tag="ACG0BW_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFK7260236.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12857..13438
                     /gene="rsxA"
                     /locus_tag="ACG0BW_003312"
     CDS             12857..13438
                     /gene="rsxA"
                     /locus_tag="ACG0BW_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFK7260237.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13438..14016
                     /gene="rsxB"
                     /locus_tag="ACG0BW_003313"
     CDS             13438..14016
                     /gene="rsxB"
                     /locus_tag="ACG0BW_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFK7260238.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAFIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14009..>15890
                     /gene="rsxC"
                     /locus_tag="ACG0BW_003314"
     CDS             14009..>15890
                     /gene="rsxC"
                     /locus_tag="ACG0BW_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFK7260239.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWDDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDITLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAI"
BASE COUNT         3787 a         4163 c         3964 g         3976 t
ORIGIN      
        1 attgctgctg cattttttta cgattatcaa tttcaaccac gatttctttg aattttttat
       61 ccgtgagcgg ataaaaccag ataatgacga atgccagcag cataaatccg caaggtacta
      121 aggcaattga tgtgcggatg cccataataa cttccggcgc ttgcacctga ttggcaatat
      181 atccgcttaa tcccaaaata aaggcaggaa ttgaacctcc gattgcctga ccgcatttac
      241 gggtaaatga gaatagtgaa taggtgagcc cttctattcg cacgccggtc aggtattcac
      301 cgtattctac agtatcagct tccagtgccc acatcacggt catggtaacg ccctgaccaa
      361 ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagaccgaa acccagaaga
      421 acagcagata accgcagctt cccagcaaag cgccaatcag gaaggtattc tttttaccga
      481 tcctcgcgac catccccggc accagcggtg ccgatgccac agtaccgacc aggttttgca
      541 ccagtaccag cacagtgaac agcccggtat catttaacac atagcgcacg tagaacaacg
      601 acgaggcgct gaccgcgaag gtcgaaatca gcacacacag cgcaccgatg cacaacataa
      661 acagcgggcg attccgtttc agggtttgca gactgatctt caatgacggc tgcgccacga
      721 tacgtaccac attttcacgc gtcgatttga agcagatgaa gtaaagcacc attccggcaa
      781 tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg ctagagttct
      841 taatgctcgg tcctatcaga aatgccaggc agacaaaggt caatgaagcg gcaatcccac
      901 gagccgcgcc cagacgggcg cgggattgtg gttgttgggt cattgcggta gcaagtgaac
      961 cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc aaatatgcat
     1021 acactacttt gctgctatgg ctccagtcgg tcggtgccca gaataccagc acgctgaaga
     1081 tcattaacgg cgcggtaccg aagagtaaaa acgggcggaa ttttccccag cgggtattca
     1141 cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc cgcaccagta
     1201 acagcatggt gcccgccgca gcggcaccga cgccagcgac gtcggtgtag taactcaaca
     1261 ggaagagcgc ccccattgcg aaggcgaagt tattggcgac gtcaccgagg ctgtagccga
     1321 cgatggtgcg ccaggagagt tgttgattca ttgtttgcct ccctgctgcg gtttttcacc
     1381 gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg gtttgcggtc
     1441 gcgggtgaag atccctttct tgttaccgcc aacgcgcaat atgccttgtg aggtcgcaaa
     1501 atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa agacgcggtg
     1561 atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt acattgagtg
     1621 cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat gcagtttctc
     1681 ctgccaggcc agcagttctt tttccagtac cttctctgcc gtttccaaat cgccgctttg
     1741 gacataccaa ccgtaataac ggttcaggca cagcacatca aagagatcgc tgatggtatc
     1801 ggtgtgagcg tcgcagaaca ttacattgac acaggtgatc ggacgcgtcg ggtcgagttt
     1861 acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac gggtatccgg
     1921 ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg caatcagctc
     1981 tttaatcgcc tgtaagtgcg cctgctgagt ttccccgttg actgcctctt cgctgtacag
     2041 ttctttcggc ttgatgcccg cttcgaaacc aatgcctaaa gagaggttaa agcctacagc
     2101 tgcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca tctcttcagc
     2161 gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta atgcgtgatc
     2221 gtgcaccatc agcacgttat cgaatccttt gccacgcaaa tccgcatctt catgacgacc
     2281 aaagccagta aaatagaacg gtttgtggtt aatcaggaac tgctcgccct tcactgccac
     2341 tgaccggatg ccgacgcgca gcgggtagat atcacactct gtctggcttt tggctgtgac
     2401 gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca ccacttgcaa
     2461 agtcccgctg gtgccttgtc cagttgcaac cacctgttga tccgcatcac gcagttcaac
     2521 gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac agtcttgcgc
     2581 gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt agagcattac
     2641 gctgcgatgg atcccggcat agttaaagaa atcatggaag taagactgct ttttcttgcc
     2701 gttttcatcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt cattgttcac
     2761 acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat cggcttcaaa
     2821 tggcgtatag ccgccctgat gctccatcac ttcctgatta tttacccaca ctttgccgta
     2881 atgagtgacc gcatcgaaac gcagcacgat acgctggccc gcccaacctt tcggtataaa
     2941 gacttcgcgc tgataccaga cgttgcccac ataattacga atatctgcat cggcgaactg
     3001 atcgttaaaa ctgcccggca cagcaattgc ccggctttct tgtaacacgc tttcccacca
     3061 acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc cgtcgagttt
     3121 tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct cattaagata
     3181 aaaatactgg tcaaccttta atctgattag ttaaccaatt ctgtgttgca cacagttatc
     3241 tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg atcaaaaaac
     3301 caaaaactgc aatacccaca gcgtgatata gatcgcatta atctttaaaa cggcgttgac
     3361 agaagccata aaataagcaa ccgaacgttc gttcgagcat aatgggaacg ataaactctc
     3421 atgacgtatg gctacagtga atattttggg gggaattctc cgaacccggg aaggataatt
     3481 tttgcacaga gaaattacga tatgaaacgc gggctcagtt gtcaccagcc cgcggaaaga
     3541 gagagaggat caggatgcgg ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa
     3601 cgacgcttca cgcgccagat cttccgcttc gatatcggcc agtacaccgt aggtcagatc
     3661 cagcagtaaa cgtgaagtca ttgccgtgtt gatgtctggg cttatctcgc ctttttgctg
     3721 ggcatcacgc atccgctggg caacaaattc cgtgatcgtc atatgcttat ttttaagcat
     3781 ggcggcgacc tgtgggttac gcatcccttc cgccataatt tcgaccacca gcgcccgttg
     3841 tccaaaggct tcatgggtga gagagacaat agactcgacc atatagtcaa cgaaatgaat
     3901 cccttcaatc ggttcccgga aacgggccag cgccctctcc tggtcctgta agataatcgc
     3961 ctgaatcagg gcttctttgg agatgaaatg gtgatagagc gtcccgggac taatggcgca
     4021 agatttacag atggctttca tcgaggcact gtgaaatcca ttttctgaaa aaatctctct
     4081 ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat
     4141 aaaaccatta tagttagcgt gaataactag tttaccgcgt tgtcaggtga gcgcctatca
     4201 ttgctgttga atagtgcgaa ggcacactct attcgaatac ttttcactcg gaaattggtt
     4261 aaccaatagc cgtgatttgg ctgccagtta tttagctgag acgttgatga gtttatcccg
     4321 gtaaacaaca cgcacggttg tttaccggtc cgttagtgta ttaattgagc tcctgtaccc
     4381 caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc gagcaaagga
     4441 acagcgccgc gttagcaata tcttgcggtt ggcccagacg tctaatcggt gtgtgctgta
     4501 acattttttg ttcaatttca ggtgtaataa cggatttcag ggcatcggtt aatattgccc
     4561 ccggcgcaat gccattcacc cgaatatttt tttcacccag gtcaaacgcc atgtttctga
     4621 ccagatgact ggccgcagct ttagatgatg cataggaggt catgtttata tttttatttt
     4681 ctgccgccat agaagtaatg gtcagaataa cgccaccgcc atttttttcc atttctggcg
     4741 caacaagttg tgacagatgg aaaaaagaaa acacattcag ttcataagca cggcgaaaat
     4801 ctgccattgg catatcaaac ggtttaggac caccgccacc ggcgttgtta accaggatat
     4861 caactttacc cagcttactg acggcaaagt ctgccagtgc agagagttcc tgttcggaag
     4921 taatatcaca acggcaggca aatgcctgac cacccagttg ttgaatttca tcaacaacat
     4981 ggttagctgc gtcggcgtta atatcactga ccaccacaga tgcgccagct gtcgcgaatg
     5041 taatggcaat ttctttacca atacctgcac cggcacctgt gatgatggcg cattttccgt
     5101 cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag taaaaataaa
     5161 aggcaccgcc tgtgtgccac attttttatc attaaccttg aggaaaggac actaccaggt
     5221 atagcagata aagagagggt tatttcgccg caatgagccg ggcgggaata ataagattgc
     5281 gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg atccgcaagt gtggtaccaa
     5341 gttcccgcgc tggcgtgctg gcccaggtaa caggtatatc atcaagtgtg gttggcgttg
     5401 catcggtaaa tgccgccaac gaaacctgtt gttcaaagta acgatcgact ccgctttcac
     5461 cgctttgccg ccctgctttc agcaaaccaa accatgcccc catcgcaatg gtttcgttat
     5521 agcaaaccac ggcactgatg gtcgggttat gacgtaaaag cgccgtgata gcttccgcgg
     5581 cttgcttctg gctggaagtg cactccagca cccaatcgct gtgaaacggc aggccaaatt
     5641 ttagtagagt tgcacaatag ccccccaccc gttctgcccg ggttaatgag gaactttgcc
     5701 ctcccagcca ggcgatacgc tgatgcccat tgcgaatgag atgctccgtc aacaactgtg
     5761 cagcctgcat gttatccggg cgaaccgtat caacatcatc aagataactg gcacgggaag
     5821 cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg ctgcttcctg
     5881 cagccccggc aattaccaca ccatcgaccc cctgattcag taacagtgaa aaccgctgtg
     5941 ccaactgctc gccgtcttta ccgccgtgaa gcaaaaaaac catccgtccc tgcgcttcca
     6001 gagcttccgt caatccggcc gttaattcgg cgtaaaacgg cgcagataaa tcacgaacga
     6061 tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctgacga ttgcgcacaa
     6121 atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag attcgccctt
     6181 tgccacttag caccagcgaa acggtactta ccgacacgcc cgcagccagc gcaacatcat
     6241 gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt atgtgacaga
     6301 taaaacgttt taccttttat ttatcttata cccgctatta tcgttgcgta atgtgattta
     6361 tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg aatccagatt
     6421 gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca cgctgtggga
     6481 gttcttccag cagttaggca aaaccttcat gttacccgtg gcattattgt cgttctgcgg
     6541 cattatgctc ggcattggta gttctcttag cagccatgat gtcatcaccc tgatcccggt
     6601 cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg gctcgtttgc
     6661 ttttagtttc ctgccagtca tgttctgtat cgccatcccg ctgggcctgg cacgcgaaaa
     6721 caaaggcgta gcggcattcg ctggcttcgt cggttatgcg gtaatgaacc tcgcggtaaa
     6781 cttctggttg accaacaaag gcattctgcc aaccactgac gccgccgttc tgaaagccaa
     6841 taacatacag agcattcttg ggatccagtc gatcgatacc gggatcctcg gtgcggtgat
     6901 cgccggtatt atcgtctgga tgctgcatga gcgattccat aatatccgcc tgccggatgc
     6961 gctggcattc ttcggcggta cgcgcttcgt accaatcatc tcctcgctgg tgatgggcct
     7021 tgtcggtctg gtgattccat tagtctggcc aattttcgcc atgggtatta gcggcttagg
     7081 ccatatgatc aacagcgcgg gtgatttcgg accgatgctg tttggtaccg gtgaacgtct
     7141 gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta ccgacgcagg
     7201 cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct tccaggcgca
     7261 attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc tttcgcaagg
     7321 taaaatgcct gcgtttctcg gcggcctgcc aggtgcagcg ttagctatgt atcactgcgc
     7381 gcgcccggaa aatcgccata aaattaaagg tctgcttatt tctggcctga tcgcctgtgt
     7441 cgttggcggc acgaccgaac ctctggaatt cctgttcctg ttcgtagcgc cagttctgta
     7501 tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc tcggcgtcac
     7561 catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt tgcatggtct
     7621 gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg tttactacgt
     7681 catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg atagcgaagt
     7741 tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt acaacgttcc
     7801 ggcaatcctc gaagccttag gcggtgccga caatattgtc agcctcgata actgcattac
     7861 ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac tgaaggacaa
     7921 tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta tcgggccaca
     7981 agttcagtca gtaaaagatg aaatggccgg tctgatgaat actgtccagg cataaggata
     8041 agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta cgcagtggga
     8101 ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt cagacatgga
     8161 ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc acggcgtgtt
     8221 tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact ggttttccac
     8281 ccagcattac accgcaattg atacgcagtc ggtggtgtat ggcccttctg ttatctacat
     8341 ggtctcggaa ctgattcgtc agtggtctga aaaaggtgaa ggcgtggtga tccacacacc
     8401 cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa tgcccgtcgc
     8461 tttagagaag caggctgacg gctggttttg cgatatgggc aagctggaag ccgtgttggc
     8521 gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg ggaaagtgtg
     8581 gacgtgcgat gagctggaga tcatggctga cctgtgcgag cgtcatggtg tgcgggttat
     8641 ttccgatgaa atccatatgg atatggtttg gggcgagcag ccgcatattc cctggagtaa
     8701 tgtggcgcgc ggagactggg cattgctaac gtcgggatcg aaaagtttca atattcccgc
     8761 cctgaccggt gcttacggga ttatagaaaa tagcagcagc cgtgatgcct atttatcggc
     8821 actgaaagga cgtgatggcc tttcttcccc ttcagtactg gcgttaactg cccatatcgc
     8881 cgcctatcag caaggcgcgc cgtggctgga tgccttacgc gtctatctga aagataacct
     8941 gacgtatatc gcagataaaa tgaactccgc gctccctgaa ctcaactggc agatcccgca
     9001 atccacttat ctggcctgga ttgatctacg tccgttgaat attgacgaca acgcgttgca
     9061 aaaagcgctt atcgagcaag agaaggtcgc gatcatgccg gggtatacct acggtgaaga
     9121 aggtcgtggt tttgtccgtc tcaatgccgg gtgcccgcgt tcgaaactgg aaaaaggtgt
     9181 tgctggatta attaacgcca tccgcgctgt tcgttaaccc caattgcgca acgtaaaaaa
     9241 tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg cacttttata
     9301 tccagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat atccatcgcc
     9361 accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag tataatatct
     9421 cgcttcctgc acaatccctg gaaacactga ttccccacgt tcaggtcatt gccaacgaac
     9481 ccgatctggt gagctttctg accaaacttg actggggcgt taaagttctc gcctctcttg
     9541 atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtcac ggcctgcact
     9601 atgtcgagct gcgtttttca ccaggctaca tggcaatggc acatcagctg cctgtagcgg
     9661 gtgttgtcga agcggtgatc gatggcgtac gtgagggttg ccgcaccttt ggtgtgcagg
     9721 cgaagcttat cggcattatg agccggacct tcggcgaagc cgcctgtcag caagagctgg
     9781 aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt gatgaacttg
     9841 gtttcccggg aagtctgttc ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata
     9901 ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat ctggcaggcg attcgtgaac
     9961 tgggtgcgga gcgtattgga catggcgtaa aagccattga agatcgggcg ctgatggatt
    10021 ttctcgccga gcaacaaatt ggtattgaat cctgtctgac ctccaatatt cagaccagca
    10081 ccgtggcaga gctggcggca catccgctga aaacgttcct tgagcatggc attcgtgcca
    10141 gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa tataccgttg
    10201 ccgcgccggc tgctgggtta tcccgcgagc aaatccgcca ggcgcagatt aatggtctgg
    10261 aaatggcttt cctcagcgca gaggaaaaac gcgcactgcg agaaaaagtc gctgcgaagt
    10321 aacaaaaatg gatggtgcta attgcaccat cctcttttca cgcaaggcac agcgtcgcgc
    10381 gatgtttggc ggattcgatg cccagctcaa tcaactccat tacctggatt gcctgacttg
    10441 ccggaaccgg attttcacca tcgccattta acgcatcacg aatagccgca tagtaagccg
    10501 gataattccc tggtactgtc aacagcgttt cttcgacacg ttcctcacct tccacgcggg
    10561 taagtacgcc atcacgcata tcgtagcccc agtcttcctg cggcagacgc tcgccatttt
    10621 tcagacgttc ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca
    10681 cgatataacg tgctgactca gcggctgcca gcatggtgcc gtgtaaaatg actcgccgct
    10741 gcggatagga caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg
    10801 ccaaatctac cgtcatgcta accggtaaac caaatagcgt aatggcctga tcaagaaggt
    10861 gcggtgctaa atcgtaccaa ataccgctgc caggaccgcc ctgttcacgc caacgatcgc
    10921 gcacctgcgg acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagtacgc
    10981 cttccgcgag caaacctttt agcgtcagga aatcgctatc ccagcgacgg ttatggaata
    11041 cagacagcac acgccccagg ctttttgcca gcgcttccag ctcgcgcgct tgtgacagtg
    11101 tcacggtaaa gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta
    11161 acgggaaatg ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa
    11221 acagatgctt cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt
    11281 catcgctgct ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat
    11341 ggaaggtttt gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt
    11401 cattgcctct cattgaagta tgatggctat ttgacactat cctttaccct cgctcaacag
    11461 tttaataacc tgccagcaat aagggatgtt gtttaactta agtcaaaaaa atagcgaatt
    11521 ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct ggagctatta
    11581 ttgctaagta acatttaccc cctgaagtta atggatcaat caacagagat gtgggctgta
    11641 atgaatcgtc ttattgaatt aacaggttgg atcgttcttg tcgtttcagt cattcttctt
    11701 ggcgtggcga gtcacattga caactatcag ccacctgaac agagtactac ggtacaacac
    11761 aagtaagctc tgcgcttgta gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt
    11821 cggatattaa accaggaatt tattatcttg ttcgatgttg ttggtgattg tcagagatag
    11881 taaagcgcgt tactcttttc agagcagttt ttcatccctg aatatttgcc atcagcgcat
    11941 tcttattatt gtaataacac aagcgttcac ttttggtgaa attttatcat cactaataat
    12001 acctacccct ttggtattaa ttattttatg gaccttttat gactgttcag gactacttat
    12061 taaaatttcg caaaatcagt tcactcgaaa gtctggaaaa actctacgac catcttaatt
    12121 acaccctgac ggacgatcag gaactgatca atatgtatcg tgctgccgat caccgtcgcg
    12181 cagagctggt ttctggcggg cgtttgtttg acctcggcca ggtaccgaag tccgtctggc
    12241 actatgtcca ataaagaaag tagcgatcgg ttataaaagc cttataataa cgcccctaaa
    12301 attagatttg cctgtttgcg tcgctattct ggagaaccta tgaccaccac aacgccacaa
    12361 cgtattggag gctggttgct tggcccattg gcctggcttt tagtcgctct gttaagtacg
    12421 acgctggcgt tgttgctgta taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc
    12481 ggcgaacaag cactgacgac acaaatctta tggggcgttt cttttattac cgctatcgcc
    12541 atgtggtatt acacactgtg gttgaccatc gctttcttta aacgtcggcg ttgtgttcct
    12601 aaacactata ttatctggct gcttatttcc gtattgctgg cagtaaaagc gttcgctttt
    12661 tcacccgtgg aagatggcat agccgttcgc cagttactgt ttactttgtt ggcaaccgca
    12721 ttgatcgtcc cctattttaa acgctcgtcg cgggtgaaag cgacgtttgt gaatccgtaa
    12781 taaccttaca gttaacctgt tgtcgcctgc tctggattaa cggataatag gcggcttttt
    12841 tatttcaggc cgaaaaatga ctgactacct gttactgttt gtcggaactg tactggtcaa
    12901 taactttgta ctggtcaagt ttctcggtct ctgtccgttt atgggggttt ccaaaaagct
    12961 ggaaaccgcg atgggcatgg ggctggctac aacgtttgtg atgacgctgg cgtctatttg
    13021 cgcctggctt atcgatacgt ggattttgat cccgcttaat ctgatttacc tgcgcaccct
    13081 ggcgtttatt ctggtgattg ctgtggttgt gcagttcacc gagatggtgg tgcgcaaaac
    13141 cagcccggtg ctttaccgtc tgctggggat ttttttgccg cttatcacca ccaactgtgc
    13201 agtgctcggc gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc agtcggcgct
    13261 gtacggtttt tccgccgctg tcggtttctc gctggtgatg gtgcttttcg ccgccatccg
    13321 cgaacgcctt gctgtggctg atgtcccggc accttttcgc ggtaatgcca ttgcgttaat
    13381 taccgcaggt cttatgtctc tggcctttat gggctttagt ggtttggtga agttgtaatg
    13441 aatgctatct ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt
    13501 ctgggttatg cctcccgccg ttttgcggta gaagacgatc cggtcgttga gaaaattgac
    13561 gaaatcttac cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg
    13621 gaagccatca gctgtaacgg tgaaaaaatt aaccgctgcg ccccaggtgg cgaagctgtg
    13681 atgctaaaaa ttgctgagtt gcttaatgtc gagccacagc cgctggatgg cgaagcgcaa
    13741 gagctaacgc ctgcgcggat ggtggcgttt attgatgaaa ataactgtat tggctgcact
    13801 aaatgtattc aggcgtgtcc ggtagacgcc atcgttggtg ctacccgcgc catgcatacg
    13861 gtaatgagtg atctctgtac gggctgcaat ttatgtgttg atccgtgccc gacgcactgc
    13921 atctcgttgc aaccggttgc agaaacacct gactcctgga aatgggatct gaacaccatt
    13981 cccgtgcgta tcattcccgt ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa
    14041 taaaatctgg gatttcaacg gcggcatcca tccaccggag atgaaaaccc agtccaacgg
    14101 tacgcccctg cgccaggtac cactggcgca gcgttttgtc ataccactga aacaacatat
    14161 tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa gtactgcgcg gccagccgct
    14221 tacccgtgga cgcggcaaaa tgctgccagt tcacgcgccc acctcgggta ccgttacggc
    14281 tattgcgccc cactctacgg ctcatccttc agctttagct gaattaagcg tgattattga
    14341 tgccgatggc gaagactgct ggatcccgcg cgacggctgg gacgattatc gctctcgcag
    14401 tcgcgaagag ttaatcgagc gcattcatca gtttggtgtt gccgggctgg gtggtgcggg
    14461 attcccgaca ggcgttaaat tgcagggcgg cggagataag attgaaacgt tgattatcaa
    14521 cgctgctgag tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg attgcgcggc
    14581 tcaggtcgta gagggtattc gcattcttgc gcatattctg cagccacgcg aaattcttat
    14641 cggcattgaa gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc
    14701 tcacgatatt actctgcggg tgattccaac caaatatcct tctggcggtg ctaaacaatt
    14761 aacctacatt ctgaccggga agcaggttcc acatggcggg cgttcatccg atatcggcgt
    14821 attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt gccgttattg atggcgaacc
    14881 gattaccgag cgtgtcgtga ccctgactgg cgaagcaatc gcacgcccgg gcaacgtctg
    14941 ggcacggctg ggtacgccag tgcgtcattt attgaatgat gccggattct gcccctctgc
    15001 cgatcaaatg gtgattatgg gcggcccgct aatgggcttt accttgccat ggctggatgt
    15061 cccggtcgta aagattacca actgtctgtt ggctccctct gccaatgagc ttggcgaacc
    15121 acaggaagaa caaagctgca tccggtgtag cgcctgtgct gacgcctgcc ctgcggatct
    15181 tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa cacgataaag ccaccacgca
    15241 taacattgcc gactgcattg aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc
    15301 cctggtgcaa tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa
    15361 gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag gctcgccttg agcgcgaaaa
    15421 agcggctcgc cttgaacgac ataagagcgc agccgttcaa cctgcagcca aagataaaga
    15481 tgcgattgct gccgctctgg cgcgggtgaa agagaaacag gcccaggcta cacagcctat
    15541 tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt attgcagcac gggaagcccg
    15601 taaagcgcaa gccagagcga aacaggcaga actgcagcaa actaacgacg cagcaaccgt
    15661 tgctgatcca cgtaaaactg ccgttgaagc cgctatcgcc cgcgccaaag cgcgcaagct
    15721 ggaacagcaa caggctaatg cggaaccaga agaacaggtc gatccgcgca aagccgccgt
    15781 cgaagccgct attgcccgtg ccaaagcgcg caagctggag cagcaacagg ctaatgccga
    15841 gccagaacaa caggtcgatc cgcgcaaagc cgccgtcgaa gccgctattg
//
LOCUS       DAYPHN010000112        15796 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000112 DAYPHN010000000
VERSION     DAYPHN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.112"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            110..199
                     /gene="kdpF"
                     /locus_tag="ACG0BW_003315"
     CDS             110..199
                     /gene="kdpF"
                     /locus_tag="ACG0BW_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFK7260240.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            199..1872
                     /gene="kdpA"
                     /locus_tag="ACG0BW_003316"
     CDS             199..1872
                     /gene="kdpA"
                     /locus_tag="ACG0BW_003316"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFK7260241.1"
                     /translation="MTAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     VLFRLPGVSDSEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1895..3943
                     /gene="kdpB"
                     /locus_tag="ACG0BW_003317"
     CDS             1895..3943
                     /gene="kdpB"
                     /locus_tag="ACG0BW_003317"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFK7260242.1"
                     /translation="MSRKQLALFEPTLVLQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCICIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     NAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3952..4524
                     /gene="kdpC"
                     /locus_tag="ACG0BW_003318"
     CDS             3952..4524
                     /gene="kdpC"
                     /locus_tag="ACG0BW_003318"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFK7260243.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4517..7201
                     /gene="kdpD"
                     /locus_tag="ACG0BW_003319"
     CDS             4517..7201
                     /gene="kdpD"
                     /locus_tag="ACG0BW_003319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFK7260244.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7198..7875
                     /gene="kdpE"
                     /locus_tag="ACG0BW_003320"
     CDS             7198..7875
                     /gene="kdpE"
                     /locus_tag="ACG0BW_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFK7260245.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDRMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSNVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(7987..8481)
                     /locus_tag="ACG0BW_003321"
     CDS             complement(7987..8481)
                     /locus_tag="ACG0BW_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260246.1"
                     /translation="MKTKRSLVVRVALITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHDMSPQEKSSLNRG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSEKCQQELKQ
                     INTH"
     gene            complement(8686..10326)
                     /gene="pgm"
                     /locus_tag="ACG0BW_003322"
     CDS             complement(8686..10326)
                     /gene="pgm"
                     /locus_tag="ACG0BW_003322"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFK7260247.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(10352..10897)
                     /gene="seqA"
                     /locus_tag="ACG0BW_003323"
     CDS             complement(10352..10897)
                     /gene="seqA"
                     /locus_tag="ACG0BW_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFK7260248.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            11082..11846
                     /gene="ybfF"
                     /locus_tag="ACG0BW_003324"
     CDS             11082..11846
                     /gene="ybfF"
                     /locus_tag="ACG0BW_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFK7260249.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWEQYPHIVGWEKIPAWDLPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            11986..12279
                     /gene="ybfE"
                     /locus_tag="ACG0BW_003325"
     CDS             11986..12279
                     /gene="ybfE"
                     /locus_tag="ACG0BW_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFK7260250.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            12419..12949
                     /gene="fldA"
                     /locus_tag="ACG0BW_003326"
     CDS             12419..12949
                     /gene="fldA"
                     /locus_tag="ACG0BW_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFK7260251.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            13159..13245
                     /gene="uof"
                     /locus_tag="ACG0BW_003327"
     CDS             13159..13245
                     /gene="uof"
                     /locus_tag="ACG0BW_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFK7260252.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            13238..13684
                     /gene="fur"
                     /locus_tag="ACG0BW_003328"
     CDS             13238..13684
                     /gene="fur"
                     /locus_tag="ACG0BW_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFK7260253.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(13769..14095)
                     /gene="chiQ"
                     /locus_tag="ACG0BW_003329"
     CDS             complement(13769..14095)
                     /gene="chiQ"
                     /locus_tag="ACG0BW_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFK7260254.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(14145..15551)
                     /gene="chiP"
                     /locus_tag="ACG0BW_003330"
     CDS             complement(14145..15551)
                     /gene="chiP"
                     /locus_tag="ACG0BW_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFK7260255.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         3526 a         4103 c         4308 g         3859 t
ORIGIN      
        1 ttttgccatt tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag
       61 ccagaattct acccttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg
      121 cgtgatagcc ggcgtgttgc tggtgttttt attactgggt tatctggttt atgccctgat
      181 caatgcggag gcgttctgat gactgcgcaa gggttcttac tgatcgccac gtttttactg
      241 gtgttaatgg tgctggcacg tcctttaggc agcgggctgg cgcggctgat taatgacata
      301 cctcttcccg gtacagcggg tgttgaacgc gtactctttc gtttacctgg cgtctctgac
      361 agtgagatga actggaagca atatctctgt gccattctcg gcctgaacat gctggggctg
      421 gcggtgctgt tttttatgtt gctcggtcag cactatctgc cgcttaaccc acagcagttg
      481 ccggggctgt cgtgggatct ggcgctgaat actgccgtca gctttgtcac caataccaac
      541 tggcaatctt acagcggtga aaccacgttg agctatttca gccagatggc gggcttaacg
      601 gtgcaaaact ttctttctgc cgccagcggg attgcggtga tttttgccct cattcgtgca
      661 tttacccgcc agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg
      721 ttatgggtgc tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg
      781 ctgcaaaact ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta
      841 cccatgggtc cggtggcttc tcaggaagcg ataaagatgc tcggcactaa cggcggtggc
      901 ttctttaatg ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg
      961 cagatgctgg cgatcttctt gatcccaacg gcactgtgct ttgcctttgg cgaagtggcg
     1021 ggcgatcgcc gtcaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc
     1081 gtaggcgtgg tgatgtgggc agaagttcag ggtaatcctc atctgctggc gctgggtgcg
     1141 gacagcagca tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg
     1201 tttgcggtcg tgactacggc ggcttcctgt ggcgcggtga ttgcgatgca tgattcgttt
     1261 accgctctcg gtggcatggt gccgatgtgg ctgatgcaaa ttggtgaagt ggtattcggc
     1321 ggtgtcggtt ctggtcttta cggcatgatg ctgtttgttc tgctggcggt gtttattgcc
     1381 gggctgatga ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgtgagatg
     1441 aaactgaccg cgctggcgat tctggtcacc ccgacgctgg tgctgatggg cgcggcgctg
     1501 gcaatgatga ccgacgccgg acgtagcgcc atgctcaacc ctggcccgca tggttttagc
     1561 gaagtgctgt acgccgtgtc atccgccgct aacaacaacg gcagcgcctt tgccggatta
     1621 agcgccaact ctccgttctg gaactgttta ctggcgttct gcatgtttgt tggtcgcttc
     1681 ggggtgatta tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc
     1741 gccagctccg gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc
     1801 gtgttgctgg ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa
     1861 tatctctcct gatgatattg agtgagcact gaatatgagt cgtaaacaac tggcgctatt
     1921 cgaaccaaca cttgtccttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc
     1981 gcaatggcgc aatccggtga tgtttatcgt ctggatcggc agtttgctga ccacctgtat
     2041 ttgcatcgca atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag
     2101 cggctggctg tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg
     2161 cagtaaagcg caggccaaca gtctgaaagg ggtgaaaaaa actgcctttg cccgcaagct
     2221 gcgtgagccg aaatatggcg ctgcggcgga caaagttccc gccgatcaac tgcgtaaagg
     2281 cgatatcgta ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg
     2341 gggcgcatcg gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc
     2401 cggcggcgat tttgcctccg tcactggcgg cacgcgtatt ctttctgact ggctggtgat
     2461 tgagtgcagc gttaaccccg gcgagacatt tctggatcgg atgatcgcga tggtggaagg
     2521 cgcgcagcga cgtaaaacgc caaacgagat tgccctgacc atcctgctga ttgccctgac
     2581 tatcgtcttt ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc
     2641 agtcagcgta acggtactgg tggcgctgct ggtctgtctg atcccaacga ccattggcgg
     2701 tctgttgtca gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc
     2761 caccagcgga cgtgcagttg aagcggcggg tgacgttgac gtcctgctgc tggataaaac
     2821 cggcaccatc acgctaggta accgtcaggc atcggagttt atccctgcgc agggcgtaga
     2881 ggaaaaaacg ctggccaacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg
     2941 tcgcagtatt gtgatcctcg ccaagcagcg ttttaatctg cgcgagcgcg atgtgcagtc
     3001 gctccatgcc acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga
     3061 caaccgcatg atccgtaaag gttctgtcga tgccattcgc cgccatgttg aggctaatgg
     3121 tggtcacttc cctgccgatg tcgatcaaaa agtcgatcag gttgcgcgtc agggggccac
     3181 gccgctggtg gtggtggaag gttcccgtgt gctgggcgtt attgcgctga aagatatcgt
     3241 caaaggcggt attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat
     3301 gattaccggc gataaccgtc tgactgccgc cgccattgct gcggaagcgg gcgtcgatga
     3361 ttttctcgcc gaagcgacac cggaggccaa gctggcattg attcgtcagt atcaggcgga
     3421 aggccgtttg gtggcgatga ccggcgacgg caccaatgat gctccggcgc tggcgcaggc
     3481 agatgtcgcg gtagcgatga actccggtac tcaggcggcg aaagaagcgg gcaatatggt
     3541 ggatctcgac tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct
     3601 gatgacccgt ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc
     3661 cattattccg gcggcgttcg cggcaacata tccacagtta aatgcgctga acatcatgcg
     3721 cctgcattcg cccgactccg caatcctcag tgcggtcatt ttcaacgcct tgattatcgt
     3781 ctttttgatt cccctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat
     3841 gttgcgccgt aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat
     3901 caaagtcatt gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgcgtgga
     3961 ttacgtccgg cattatcgac atttatcttt ctgttattga ttactggcgg cgtttacccg
     4021 ctgctaacca ccgcgctggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc
     4081 cgtgaaggtg atacggtgcg cggttcggca ttaatcgggc agaattttac cggcaacggc
     4141 tattttcatg gtcgcccgtc ggcaacggca gaaatgccct ataatccaca ggcttctggc
     4201 gggagcaatc tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct
     4261 gcattacggg ccgctaaccc tgatgccagc gcgaacgttc cggttgaact ggtgacggca
     4321 tcggcaagcg ggctggacaa taacatcacc ccgcaagcgg cagcctggca gatcccacgc
     4381 gtggcgaaag cgcgtaatct cagcgttgaa cagctcacgc aactgatcgc aaaatacagc
     4441 caacaaccgc tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg
     4501 gcgctggata agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga
     4561 acaaactgcc gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gcgcaggcgt
     4621 cgggaagacc tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat
     4681 tgtggttggc gtggtagaaa cccacgggcg gaaagatacc gccgcaatgc tggaagggct
     4741 ggctgttctg ccgttaaaac gccaggcgta ccgtgggcgg cacatcagcg agtttgatct
     4801 cgatgccgcc ctcgcccgcc gtccggcact gatcttaatg gacgaactgg cgcacagtaa
     4861 tgcgccaggt tctcgtcatc ctaaacgctg gcaggacatc gaagagctgc tggaagctgg
     4921 cattgatgtt ttcacaaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag
     4981 cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga
     5041 cgacgtggtg ctggtggact tgcccccgga cgatctgcgt caacggctga aagaaggtaa
     5101 agtctatatt gccgggcagg cggaacgcgc tattgaacat ttttttcgca aaggcaatct
     5161 gatcgccctg cgcgaactgg cactgcgccg tactgccgat cgcgtagatg agcaaatgcg
     5221 cgcctggcgg gggcatcctg gcgaagagaa agtctggcac acgcgcgacg cgatcctttt
     5281 atgcatcggg cataacaccg gcagcgaaaa actggtccgc gcggcggcgc ggctggcgtc
     5341 acggctgggt agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc
     5401 ggagaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcaga
     5461 gacggcaaca ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca
     5521 taatcttggc aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac
     5581 gtttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga
     5641 tgaaccaccc gcccgcacga taaacaacgc gccggatagc cgctcgttta aagacaagtg
     5701 gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat
     5761 tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctacttgg
     5821 cgtggtggtg gtggcgctat tttatgggcg ctggccttcg gtggttgcta ccgtcattaa
     5881 tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt
     5941 gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac
     6001 tgctggcgtt cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt
     6061 atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag
     6121 cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa
     6181 cggtaaattg cagccgttaa cacatccgca aggaatgacg ccgtgggacg atgccatcgc
     6241 gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt
     6301 accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga
     6361 accggggaat ctgcgccagt tgatgatccc ggaacagcaa cgcctgctgg agacgtttac
     6421 gctgttagtc gccaatgccc ttgagcggct gacactgacc gccagcgaag aacaggcgcg
     6481 gatggcaagc gaacgagaac agatccgcaa cgccctgctg gcggcgcttt cgcatgattt
     6541 acgcacgccg cttacggtgc tgttcggtca ggcagaaatc ttaacgctcg atctggcgag
     6601 cgaaggatcg ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacacgac
     6661 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
     6721 gaaagagtgg ttaacgctgg aagaagtggt cggcagcgcg ctgcaaatgc tggaaccggg
     6781 tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
     6841 gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat acgcgggtgc
     6901 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
     6961 ggataatggc cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg
     7021 cgggaataaa gagtcggcgg tgccgggtgt agggcttggg ctggcaattt gtcgggcgat
     7081 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
     7141 tcgtgttaca cttccccagc aaactgcccc tgaacttgaa gaatttcatg aggatatgtg
     7201 acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg
     7261 gagggcgata ggatgcgcgt ctttgaggcc gaaacgctgc aacgcggctt gctggaagcg
     7321 gctacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
     7381 gagtttatcc gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct ttccgcacgt
     7441 agcgaagaga gcgataaaat cgccgcgctg gatgccggag cggatgatta cctgagtaag
     7501 ccatttggta ttggcgaatt gcaggcccgt ctgcgcgtcg cattacgccg ccactctgcc
     7561 accaccacgc ccgatccgct ggtgaaattt tccaatgtta ccgtcgattt agcggctcga
     7621 gtgatccatc gcggtgagga agaggtgcat ctcaccccga ttgagttccg cctgctggcg
     7681 gtgttgctta ataatgcggg aaaagtgctc acccagcgcc agctacttaa ccaggtgtgg
     7741 gggccaaacg cggtcgaaca cagtcattat ttgcgtattt atatggggca tctgcgacaa
     7801 aaactggagc aagatcccgc ccgcccccgc cacttcatta ctgaaaccgg tattgggtat
     7861 cggtttatgc tttagggtct gaattgccgg atgcggcgtg aacgccttat ccgacctaca
     7921 catcgtcccg taggcatgat aagacgcgtc agcgtcgcat caggcatggc actcatcgcg
     7981 atatgtttaa tgcgtattta tctgtttcaa ctcttgctgg catttttctg actggcagcg
     8041 tcgcaactcg ataatcccga catgcatcat cgagccaaaa aatccgcttt cgctgacata
     8101 atcctcatta acaatcggtc gagtgccagg cttgccctgg caaacccgca taaaaaatga
     8161 ggtgtagcac tgccagccac gatttaaact gctcttttct tgtggtgaca tatcgtgaag
     8221 ataagtaagt tctttttcgt tcccgcctga caactgccag cggcgggtat tatcaggtaa
     8281 gttatcgcca cggcaatttg tcttacaccg ggaaggcgca atatttaccg caatgctacc
     8341 cgttaatcct ctggttttat ctttaggggt cagtaaccag tcaccagcta atgatgattg
     8401 ttcaggttgg gcgcatgctg tcagtagtag tgtggcggta attaatgcca ctctgacgac
     8461 cagactcctt ttggtcttca ttttgaattc ccttgagcta ttcagtttgt tggcaaagtg
     8521 ctcgttgttc atgccggatg cagcgtgaac gccttatccg gcctacaaaa tagcgcaaat
     8581 tcaatatatt gcaccatcct cgcaggcctg ataagcgtag cgcatcaggc agtcataagt
     8641 aaaaaagggc gatcttccga ccgccctttt tcttatttaa tgtgtttacg cgtttttcag
     8701 aacttcgcta acaatctcaa ccgcttcttt ctcgatctgc ttgcgatgct cttcaccgag
     8761 gaagctttcg caataaatct tgtacgcgtc ttccgtgcct gacggacgcg cggcgaacca
     8821 gccgttgtca gtcatcactt tcagaccgcc gatagaagca ccgttgcccg gtgccgccgt
     8881 caggcgcgcg gtgatcgggt cgcctgccag ggtgctggcg ctcaccattt ccggagacag
     8941 cttagacagc gccgcttttt gtgcggaagt cgcagctgcc tgcaaacggt tgtagctcgg
     9001 cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc ggattcttac cggtgacagc
     9061 ggtgatttcc gccgccagca gacacatgat gatgccgtct ttgtcggtgg accacggcgt
     9121 gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc cgaagctgcc
     9181 gtcgaacaga ccatcaacaa accatttgaa acctaccggg acttctacca gcttacggcc
     9241 caaatcattg accacacggt cgatcatcgc agaagaaacc agcgttttac cgacagcaac
     9301 atctttgccc cactgcggac gatgctggaa caggtagttg atcgccaccg ccaggtagtg
     9361 gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt
     9421 agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga
     9481 ggagcagtcc atacggatcg cgccgtcttt atcgaggtgc ataaagcgga aggtttgatc
     9541 gacctgatcg ttaacgatgg tcaggttgag attgtaatac tcgccaatgc gcttccagta
     9601 ttcgataccg gaaccaccca gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc
     9661 cgccatatca acgatatcag ccagaccttc cacaaacggc tgcaccagat cctgctcttt
     9721 cacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc
     9781 agccatcagt gcgttggccc tgtcttccac cactttagtg acgttggtat cagccgggcc
     9841 accatttggc ggattgtact tgataccacc atcttccggc gggttatgag acggtgtaat
     9901 cacgataccg tctgccagcg ggccaccttt tttattgtga accaggatgg cattggaaac
     9961 ggcaggcgtc ggagtgaagc cattgttttc ctgcacaata acatctacgc cgttcgctgc
    10021 cagcacttcc agtacggaaa tgaacgcagg ctcggacaga gcgtgagtat ctttacccac
    10081 atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc
    10141 cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc
    10201 gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt
    10261 cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat
    10321 tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca
    10381 attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg
    10441 tgttggtgtt ggtgatcacc caatacggcg tgccaggcac atgtttcggc ttggtctgat
    10501 taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca
    10561 acgattccgt tgcctcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca
    10621 gcatgaagcg attgaccgct cgcttttgct ctgcgtattc atccgaaagc agaagttcac
    10681 gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg
    10741 acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca
    10801 acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat
    10861 agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag
    10921 ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc gtcaagcctg
    10981 gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac
    11041 ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg
    11101 cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccac ggtctgtttg
    11161 gcagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc
    11221 aggttgatat gcgtaaccac ggtctttcac cgagagatcc ggtgatgaat tacccggcga
    11281 tggcgcagga tcttgttgat actctggatg cacagcagat cgacaaagca acatttatcg
    11341 gtcactccat gggcggtaaa gcggtaatgg cacttactgc actggccccc gatcgcatcg
    11401 ataaattggt ggcgattgac atcgcgccgg tcgactatca cgtacgccgt catgatgaga
    11461 ttttcgcggc tatcaacgcc gtaagtgaat cggacgcaca aactcgccag caagcagcag
    11521 caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg
    11581 ttgacgggga gtggcgcttt aacgtgccgg tattgtggga acagtatcca catattgtgg
    11641 gttgggagaa aatcccggca tgggatctcc ctgccctgtt tatccctggc ggcaattctc
    11701 cgtatgttag cgagcagtac cgcgatgatt tactggcaca atttccacag gcacgggcgc
    11761 atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg
    11821 ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgc ccgcgtcgct
    11881 ttccgcgcgc gggcgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc
    11941 cgggcagagg cctggctgat tgtttccccc gaagtcacca aaatcatggc caaagaacaa
    12001 acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaact
    12061 aatccgcttt cgcgcgatga acagctgcgt attaataaac gcaaccagct aaaacgcgac
    12121 aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcgctg
    12181 aacgagctgg cggaatcgcg caatatgagc cgtagcgaac tgatcgaaga gatgttaatg
    12241 cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac
    12301 agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct atgattgcct
    12361 ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta tttcactcat
    12421 ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat
    12481 gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa
    12541 agaagatctg gaagcatatg acattctgct gctgggcatc ccaacctggt attacggcga
    12601 agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt tcaatggcaa
    12661 actggttgcg ctgtttggct gtggtgacca ggaagattac gctgaatact tctgtgacgc
    12721 attgggcacc atccgcgaca tcattgaacc gcgcggtgca accattgttg gtcactggcc
    12781 aactgcgggc taccatttcg aagcgtcaaa aggtctggca gatgacgacc actttgtcgg
    12841 tctggctatc gacgaagacc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt
    12901 taaacagatt tctgaagagt tgcatctcga cgaaattctc aatgcctgat gtgatgcggc
    12961 gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt
    13021 tgtaactttt gctgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta
    13081 aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt
    13141 aggcgtggca actctataat gatacgcatt atctcaagag caaattctgt cacttctcct
    13201 aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca ataccgccct
    13261 aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag ttcttcagga
    13321 gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga
    13381 agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt
    13441 cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc aacatcacca
    13501 cgatcacctg atctgcctcg actgcggcaa ggtaatcgaa tttagtgatg actccatcga
    13561 agcgcgtcag cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta
    13621 tctttacggt cactgtgccg aaggcgattg ccgcgaagat gagcacgcgc acgaaggcaa
    13681 ataagccagc ctgaatgaga aaaagccaac ctgcaggttg gcttttttat gcaagggaaa
    13741 taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt tcctgccaaa
    13801 ctttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca cgcagacact
    13861 gctgataatc cagtacgcgt acactttcct ggtcggcaaa ctgctgatgt tgtttctctt
    13921 tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttctggcgaa ccttgtgcgg
    13981 tgttgataca ggcgctgtat gcctctttca gacggctgtc ttcctgtggc gcggttgatt
    14041 gcgcacaagc caccagcccc gatgccatca tggcgatgag aatcagtttt ttcatagcaa
    14101 attcctcaaa cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat
    14161 ggtgcaatta ccataaattt cacgtcacgc tcatcctgga agatgttacc gtaaccgccg
    14221 ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg
    14281 cctttggcgc ggccatcctg aatggtgtac accgcatcca ggctgtaggc agactcttca
    14341 atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtagc gggtttagca
    14401 tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca gtttttcaag
    14461 tcatacatcg caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc agaacggtta
    14521 tcccaccaga tatccaggcg accgttggag gaagcgtagg ttggagtcat acgttgcagg
    14581 aagtatccct gctgaccgtc ggctttaacc caggtgcctt ccagacgcaa atccactaca
    14641 tcagccgccc ggtaaccaaa ggtcaatgct tgcagccagg cagtgccgtc gtaaaggtcg
    14701 ttgacgctgc gatcgtcaac tttatcgcgc gtaccgtaga actggtagct ggtggttaac
    14761 gggctaccgg cgatatcaaa tttgtagctg gctttggcaa agtattgatc gatataccct
    14821 tccgcctgac caaatgctgc ttccagtacg aagttatttt tgaagtcgta tttcgcaccg
    14881 atggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc atccatttcc
    14941 agatgccacg gtgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccagca
    15001 tcgccgtaat caaaattcgc cccggcttcc gccccctgat aagtacccgg cataaagctc
    15061 cagtgcggcg ctaacagcgt ttgaccagtt ggctgaatgt aacctgccct cgcccaaacc
    15121 ggaccgtatt taaatttggc cgctgcttta tacaggctta taccgctttt atcgccggac
    15181 cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg gtgagagctg
    15241 tcaccgtttt ccgccatttc aatcgccgta aacgcggcaa tatcaaggcc gaacatatca
    15301 gcagcataac cggactgaaa atcgaggttg gcgttccagg tggagtgaga aaggttggtt
    15361 ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca gtaatagata
    15421 ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg cattgccata
    15481 aagcccgaca ttgctgtaac accggcgata gccagcgcca gcgtactacg tttgccacta
    15541 aacgtacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag gtgaacagcg
    15601 aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac
    15661 tatttttcgc gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca
    15721 accgcacata ttttgatgat ttttgttaca aggcttaagt ttgcagttga aaggcttgca
    15781 gaagaataaa aacgtg
//
LOCUS       DAYPHN010000113        15381 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000113 DAYPHN010000000
VERSION     DAYPHN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.113"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(93..356)
                     /gene="cedA"
                     /locus_tag="ACG0BW_003331"
     CDS             complement(93..356)
                     /gene="cedA"
                     /locus_tag="ACG0BW_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFK7260256.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            539..2800
                     /gene="katE"
                     /locus_tag="ACG0BW_003332"
     CDS             539..2800
                     /gene="katE"
                     /locus_tag="ACG0BW_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFK7260257.1"
                     /translation="MSQHNEKNPHQHQSPQHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMMLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     MWSRIPKIDKIPA"
     gene            complement(2847..3596)
                     /gene="chbG"
                     /locus_tag="ACG0BW_003333"
     CDS             complement(2847..3596)
                     /gene="chbG"
                     /locus_tag="ACG0BW_003333"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFK7260258.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRADRQMVFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSAYCL
                     PRLTELDVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3609..4961)
                     /gene="celF"
                     /locus_tag="ACG0BW_003334"
                     /gene_synonym="chbF"
     CDS             complement(3609..4961)
                     /gene="celF"
                     /locus_tag="ACG0BW_003334"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFK7260259.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFNIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5066..5908)
                     /gene="chbR"
                     /locus_tag="ACG0BW_003335"
     CDS             complement(5066..5908)
                     /gene="chbR"
                     /locus_tag="ACG0BW_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFK7260260.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5916..6266)
                     /gene="chbA"
                     /locus_tag="ACG0BW_003336"
     CDS             complement(5916..6266)
                     /gene="chbA"
                     /locus_tag="ACG0BW_003336"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFK7260261.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRIALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6317..7675)
                     /gene="chbC"
                     /locus_tag="ACG0BW_003337"
     CDS             complement(6317..7675)
                     /gene="chbC"
                     /locus_tag="ACG0BW_003337"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFK7260262.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7760..8080)
                     /gene="chbB"
                     /locus_tag="ACG0BW_003338"
     CDS             complement(7760..8080)
                     /gene="chbB"
                     /locus_tag="ACG0BW_003338"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFK7260263.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8379..8717)
                     /gene="osmE"
                     /locus_tag="ACG0BW_003339"
     CDS             complement(8379..8717)
                     /gene="osmE"
                     /locus_tag="ACG0BW_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFK7260264.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8919..9746
                     /gene="nadE"
                     /locus_tag="ACG0BW_003340"
     CDS             8919..9746
                     /gene="nadE"
                     /locus_tag="ACG0BW_003340"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFK7260265.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9976..10863
                     /gene="cho"
                     /locus_tag="ACG0BW_003341"
     CDS             9976..10863
                     /gene="cho"
                     /locus_tag="ACG0BW_003341"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFK7260266.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQQAS"
     gene            complement(10823..11398)
                     /gene="ves"
                     /locus_tag="ACG0BW_003342"
     CDS             complement(10823..11398)
                     /gene="ves"
                     /locus_tag="ACG0BW_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFK7260267.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11601..12086)
                     /gene="spy"
                     /locus_tag="ACG0BW_003343"
     CDS             complement(11601..12086)
                     /gene="spy"
                     /locus_tag="ACG0BW_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFK7260268.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12416..13384)
                     /gene="astE"
                     /locus_tag="ACG0BW_003344"
     CDS             complement(12416..13384)
                     /gene="astE"
                     /locus_tag="ACG0BW_003344"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFK7260269.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWLWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQTPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGENVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13377..14720)
                     /gene="astB"
                     /locus_tag="ACG0BW_003345"
     CDS             complement(13377..14720)
                     /gene="astB"
                     /locus_tag="ACG0BW_003345"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFK7260270.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQVQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERQAVNPAVMMNDTLFN
                     VLNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14717..>15381)
                     /locus_tag="ACG0BW_003346"
     CDS             complement(14717..>15381)
                     /locus_tag="ACG0BW_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFK7260271.1"
                     /translation="AGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTPGKWDDEPQP
                     FIGGLISDQAAQQVVTAWQQLEAMGGRPLLAPCLLQAGTSLLTPGIIEMTGVAGVPDE
                     EVFGPLLRVWRYDNFDEAIRMANNTRFGLSCGLVSPAREKFDQLLLEARAGIVNWNKP
                     LTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEV
                     VR"
BASE COUNT         3977 a         4046 c         3585 g         3773 t
ORIGIN      
        1 ctttttgtta caggtggttt gaatgaatgt agcaatgaaa tacagaattt caggccatgt
       61 aactcccggc aaaaccggga ggtacgtaat ctttactcag tcacttcccc ttcctggcgg
      121 atctgatttg cccaacgctg ggcggattca ggcacagtaa acgccggtga gcgcagaaat
      181 gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac
      241 gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata
      301 atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta
      361 aaaatcagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat
      421 ttcataacac cgtttccaga atagcctccg aagcgggatc tggctggtgg tctatagtta
      481 gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat
      541 gtcgcaacat aacgaaaaga acccacatca gcaccagtca ccacaacacg attccagcga
      601 agcgaaaccg gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga
      661 accgacaccg ccaggtgcac aacctaccgc cccagggagc ctgaaagccc ctgatacgcg
      721 taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac
      781 cactaatcag ggcgtgcgca tcgccgacga tcaaaactca ctgcgtgccg gtagccgtgg
      841 tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg
      901 catcccggaa cgtattgttc atgcacgtgg atcagcagct cacggttatt tccagccata
      961 taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc
     1021 agtatttgtt aggttctcta ccgttcaggg tggtgctggc tctgccgata ccgtgcgtga
     1081 tatccgtggc tttgccacca agttctatac cgaagagggt atttttgacc tcgttggcaa
     1141 taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt
     1201 aaaaccagaa ccgcactggg caatcccaca agggcaaagc gcccacgata ctttctggga
     1261 ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg
     1321 gatcccacgc agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa
     1381 tgccgaaggg aaggcaacgt tcgtacgttt ccactggaaa ccactggcag gtaaagcctc
     1441 actcgtttgg gatgaagcac aaaaactaac cggacgtgac ccggacttcc accgccgcga
     1501 gttgtgggaa gccatcgaag caggcgattt tccggaatac gaactgggct tccagttgat
     1561 tcctgaagaa gatgaattca agtttgactt cgatcttctc gatccaacca aactcatccc
     1621 ggaagaactg gtacccgttc agcgtgttgg caaaatgatg ctcaatcgca atccggataa
     1681 cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga
     1741 cttcaccaac gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag
     1801 tcgtcttggt gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca
     1861 taatttccag cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga
     1921 accgaactcg atcaacgata actggccgcg cgaaacaccg ccggggccga aacgtggcgg
     1981 ttttgaatca taccaggaac gcgtggaagg aaataaagtt cgcgagcgca gtccatcgtt
     2041 tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg
     2101 ccatattgtc gatggtttca gttttgagtt aagcaaagta gttcgtccgt atattcgtga
     2161 gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa
     2221 tctcggtatc gagctgacgg acgaccagct gaatatcacc ccgcctccgg acgtcaacgg
     2281 tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg
     2341 cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa
     2401 ggcgctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac
     2461 tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac
     2521 ggtcgatgcg gttattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc
     2581 caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc
     2641 gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc
     2701 tgacagcgcc gatggtagtt ttatggatga actgttaacg ctgatggcag cacaccgcat
     2761 gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgccc
     2821 cctggggagt gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca
     2881 cgctcagcaa tcgcatattt caacgacgct gaagtcagca catccagttc cgttaaccga
     2941 ggtaagcaat aagcactctg gcgaatcgta ttatcgataa acgccggatg acacatcact
     3001 tccagagagc gctcgccgcg atgactggag tcatcaagca cttgcaggaa cagcgactca
     3061 ctgatctctt caccgtagaa tgcactgctg aatccctggg tagtgcgcag gttgaccggc
     3121 aaatcaaaca ccatctgacg atccgcccgc aacgcaatcc cctcttcagc cgcaaacctg
     3181 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
     3241 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
     3301 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
     3361 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
     3421 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
     3481 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
     3541 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcaataagcg ttccataatc
     3601 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
     3661 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
     3721 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcagcgttg
     3781 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
     3841 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcgtacaggt catttctacc
     3901 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
     3961 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
     4021 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
     4081 aaaagttgtt tctcgacttt ctgtactacc tgtgctcgtg cgccgccttt gtagtattcg
     4141 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
     4201 aacagattta gagaacgaat taaaccttcg ctaaatggca gatcgaaaat atttttaacg
     4261 ccagatgctt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgtgatttg
     4321 ccatttacca gcacatcttt aatgaacacc atatggttga ggccgaacag atcgatagat
     4381 aaatcatcgc tgtctttcag catcagaaca tcgcgaataa acatcttcat gccgatcgga
     4441 atattacaca cgccgataaa gcgtttaaat ccggtatgac gataaacggc ttcagtgacc
     4501 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
     4561 tttacgatgt taaaaatcac cggaatggta cgcagacctt taaacagacc gcccgcgccg
     4621 ttagtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcgcgcgcc
     4681 ggtaattggc ctacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
     4741 cgatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
     4801 agatcaaaaa taatatccag tttagcttta ccaccttcga catccaccag ccataattcg
     4861 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
     4921 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
     4981 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
     5041 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatagc
     5101 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
     5161 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgttttttgg
     5221 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
     5281 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
     5341 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactgcggta
     5401 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
     5461 ccatctcaac aaactcttcc agccctgttt cgcggaaatt caatgaagaa atcactgttt
     5521 ccacataggt gagaaaagcg ttattggttc ggtatacctg cgaagctaca aagcaataag
     5581 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
     5641 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
     5701 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
     5761 ccagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
     5821 agataaacac atggaaattt ttgccattaa acaactgctg ttctcgggca gtggcaattt
     5881 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
     5941 tcaattaatt cggtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
     6001 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
     6061 gtctgtacca gatgcgcttc attcaatgct atccgtgact gatccatcat ggcttttgct
     6121 gcggcaaaat cgccctgctt cgcctgtttc agtgccgcat acgccaggct gcgcgcttgt
     6181 ccggagttga tgataagccc catcaccact tcttccagct cttcagcttc cgtttgcgta
     6241 tcgggaatat tatcgagatc catcatacat cgttcctctc tattcttacc ggcacgatta
     6301 cccgtaccgg catcgattaa aatttcaggg cgttagcgat atcttcttcg ctctcttctt
     6361 tatcaatcgc attttgtgct ttgttagcca ccacaacaaa gggcagataa attaacgttg
     6421 cgataccaag gttgaagagt gcgaccagca atgcggcgac gctaccgttg gtgttaaaga
     6481 aggctcccag accggttggc atggtccacg gtgcaatatt ggtcaccgga ggaataatgc
     6541 ccatgtagta cgctgccagg gtgattgccg ccagaatcgg ttgtaccagt acaaacggga
     6601 taaacatcac cgggttcatg ataattggca gaccaaacag aatcggttcg ttaatctgga
     6661 agatgccgga cggcagcgcc agttttgcca cctgacgata atcagcacgg cgagaggcga
     6721 taaagatagc caggatcaga cctaaagtcg caccactgcc cccaaggaaa ataaaggagt
     6781 ccagcatcgg cttggcccag atatggaagg tcttaccggc tgccagcgcc gcttcgacgg
     6841 aaccatattg ctgataggtc gcgatatttt ccagcgccca aggcgtcata atgccgttgt
     6901 ccagtgcggt cagcgccagc gcaccatgaa taccgaagaa ccagagcagt ggaacaaaga
     6961 tcacataggc ccagcccacc acgctaccca acgatgccag tggggttgag atggtatcca
     7021 taatgatctg atggaagttg gtgccccagg tattcaacgc ccaggcaata atccccatca
     7081 cggaaagaat aataaagccg ggaattaaag ccgagaagga acgcgatact gaagcaggta
     7141 cgctatcggg caatttaatg acccaattgc ggcggacaat aaaggtaaac atttctgcca
     7201 ccaccaggcc aataataatc ccggagatga tattcgcccc acctaaccag tttgcgccaa
     7261 ccgcataggc ctcaccgaca ctgtatgggg tgacggtcat aaatgctgca acggataaca
     7321 acccagccgc cagcgcatcg actttacgct cttctgccag cgccatgcca ataaagaacg
     7381 gtgccattaa agacattatc cctaatgttc cgttatatac gttgccgcca atacctttca
     7441 gaccattaag tgtttcaatg gttgaggcat ccaggcgaat acctaaggaa taaaaaaacg
     7501 acccctcccc aaagcttaga aaaacgttgt taattaatac aaacatggcc cccgcaaggg
     7561 ttaacggcat taagcgaata aagccatttt tgattgcatt aacgtgtggc tgctttccta
     7621 ttttaactgc aaaagggagg agtacctttt caagcgatgc aataacatta ctcatagaaa
     7681 aataccctta aaaaccgcaa tttaaatatt acggtattga tttatgaaat aactctttga
     7741 cgggaaaatt taaaataaat taatttgctg cggctttttt aatcgctgca accgcagcct
     7801 taagcacgcc taaaccatcg actttgccat aaagcagcga gtcaattact tcaaccggtt
     7861 tgttgggtaa caaacgctgg atttcgggca acatataagc aatctgcggc cctaataaca
     7921 cgacatcggc attctgacct ttttcaccag ccagtgtttc cggaaatgct tcaataatga
     7981 ccggaacttc atatttttct gcctgtgcgc gcatttttga taccagtaaa gaggtagaca
     8041 tgcccgcaga acaaaacaga taaatgtgtt tcttttccat aaaactgccc tcatcgacga
     8101 ttatctgtca gccagacact ccgcaagcct taacctgctt ccatgctctg ggtaacttgc
     8161 gaaaccaaca tgactttttt tgtggctgaa aggagtatac ggtatagacc tgagcaatag
     8221 tatttctttg accttataaa ttgtctctcc ttgacctgcg gttatgaccc tcttcacatt
     8281 tcgggcaaat aattcgcgaa gataattaag aactcaggcc aaaaaaaacc ggcgcaatgg
     8341 ccggtttccg ttgtaactca agctttcagg cgaagtgatt acttcgcagc ctgtggatca
     8401 gtgtcgtatt cagcacaggt ctgataaccg gagttgatga catgtccggt atcatctaac
     8461 gcgacaaagt aggtttctgc tttaccatca cgttgaccca ggatgtaggt ctggcaagta
     8521 ccacgagcat ggatcatgct cacttcagac gaaggtttac ccgcaatttg tgcaacctgc
     8581 gcccggctca tgcctttttt gacgtctttc accacaggct gtacaaactg gtctttggta
     8641 cgatcataag ccgtacaacc cgccagcatg gttaataccg ccgctgcact cagaattcct
     8701 gccatattct tgttcatatt ccgtcctctt gtttatcagc gtgttagata agcctggaat
     8761 acattgggcg ctttttcaag cccgtgaacg aaacggctcc gctttcagag gattcctgta
     8821 tgacgtttta accaccattc agcccgctgt cgcttgtcgt ttcagtagca acgggttagc
     8881 tttaaggaag ttttgtcttt tctgtctgga ggggttcaat gacattgcaa caacaaataa
     8941 taaaggcgct gggcgcaaaa ccgcagatta atgctgaaga ggaaattcgt cgtagtgtcg
     9001 attttctgaa aagctacctg caaacttatc cgttcattaa atcactggtg ctagggatca
     9061 gcggcggtca ggactccacg cttgccggaa agctgtgcca gatggcgatt aatgagctgc
     9121 gccaggaaac tggcaacgaa tcactgcaat ttattgccgt acgcctgccc tatggtgttc
     9181 aggctgacga acaagattgc caggatgcca ttgcctttat tcaaccggat cgcgtattaa
     9241 ccgttaatat caagggcgcg gtattggcca gcgaacaggc gctgcgcgaa gcgggcattg
     9301 aactgagcga ttttgttcgt ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata
     9361 gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta
     9421 ccggattctt cactaaatat ggtgacggcg gtacggatat taatccgctg tatcgtctca
     9481 acaaacgcca gggtaaacag ttactggcgg cattaggttg cccggaacac ctttataaga
     9541 aagccccaac ggccgatctg gaagatgatc gcccttctct gccagatgaa gtggcactcg
     9601 gcgtgaccta tgacaatatc gacgactatc tggaagggaa aaacctacct gaacaggtcg
     9661 ccagaacaat agagaactgg tatctgaaaa ccgaacataa acgccgtccg ccaattaccg
     9721 ttttcgatga tttctggaaa aagtaataat ttgcacatat tggtttgtgc gaaaaagagt
     9781 actttgttca cgccggatgc ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt
     9841 caatacattg caggagtcga ataggcctga caggcgtagc acgtctcacg gtgtaacctt
     9901 tgtcatcaac ccgcctcttt tttaatcgct ccccgcctgt tacactggat agataaccag
     9961 cattcggagt caacagtggt acggcgttta acttctccgc ggctcgaatt tgaagctgcg
    10021 gcaatttatg aatatcccga acatttacgt tcattcctta atgacttacc cacccgacca
    10081 ggggtgtatc tgtttcatgg cgaaagcgac accatgccgc tctatatcgg caaaagcatt
    10141 aacatccgca gccgcgtcct ttctcattta cgcaccccgg atgaagccgc catgctacgg
    10201 caatcccggc gaattagctg gatctgcacc gcaggcgaaa tcggcgctct gctccttgaa
    10261 gcgcgattaa tcaaagaaca acagccgctg tttaataaac ggttgcgccg caatcgccag
    10321 ctctgtgccc tgcaattaaa tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat
    10381 ttttcacgag cccccaacct gtttggcctg tttgccaata ggcgcgcagc tttgcaagca
    10441 ttgcagacca tcgctgatga acaaaaactt tgctatggcc tgctgggact ggaaccgtta
    10501 agccgcgggc gtgcttgttt tcgttctgca ttaaaacgtt gcgccggggc gtgttgcggt
    10561 aaagagagcc atgaggaaca tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg
    10621 gtgtgttggc cttggcaagg ggcggtggcg ctgaaagaac agcacccgga aatgactcaa
    10681 tatcatatta ttcaaaactg gctgtggctg ggggcggtta attcgctgga agaagcgaca
    10741 acgttaattc ggacacccgc cgggtttgat cacgacggtt ataaaattct ttgtaagccg
    10801 ctgctttccg gtaactatga aattactgaa cttgatccgg cgaatgacca gcaagccagt
    10861 tgatttcact gaacaacagt ttaccctgcg gttgtaaaag ccgcagggtg tgcctgccat
    10921 cgaaccagca cgcgccctgg tcggtagtga gcagtttatc gcccaattgc cacgccccgt
    10981 taatgacaaa cactaccccg ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc
    11041 tgactttcgc cttacagaca tccaggcgtg tcatgatgtt gaaatccatc gacatctgcc
    11101 ctgccgtcag tttcgctttt accacctggt ccgccgcaaa ggcaaaaggc tgtaacggtt
    11161 ttaaggtatg gttaaagcgg tctgcgcttt caaggaacat ctcgccgcct tccagcaacg
    11221 tcactatcct ttccataccg ggaaacaaag aaaactcccc attcgccgcg atggatgcaa
    11281 tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt tcgcgcgttt
    11341 caccggcagc atttcgccac agattcaccg acattttacg catatcaaag tattccatac
    11401 tcgctcccgg cccgttcttt tcattgttat cgttcaggta accgtggatg gttatcggca
    11461 actcgggtga tttacgttag tggtgatcag gcttttttta caactgtttg agaagagaaa
    11521 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct
    11581 taagtttttt ggctgaaaga ttattcagca gttgcaggca ttttaccttt tgccgctgga
    11641 cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc cggcgtcagg
    11701 atgttgtaaa ttttgttctg ggtttccatg tgcgccagca tgttagcttt gcgctgttct
    11761 tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct cgcaatgatg
    11821 tcatgcattg cgcggcgttc ttccagcggc ggacgtttca tctggtcacg ctggcctttc
    11881 atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt gaacatcatg
    11941 tcctgatgcg gaccaaactt gcctttgtgg tgcatcatcg gcttcgcgtc agccggtgct
    12001 gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag ggtagaggca
    12061 acaaacaatg cagttaattt acgcatattc tatatccttc ctttcagtta tttattacgg
    12121 ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag cgtcaattaa
    12181 tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa
    12241 aaaagaaaat ttgatggaga gtgatgagag aatattacaa cacgatgatt ttgcagagat
    12301 tatgaagaac tataccggat gactggtgat gaataaagca aataaccagg attaatctgt
    12361 attaatttat aagaaagcaa cttaataccc gcagaatgat ttctgcgggt aagtattagc
    12421 ttattttttc gagcattaat cccgcgcgta atcccaacgc taccaaagga ttagggaata
    12481 acacatactc tacatcatgg gttacggtaa aacgttcctc cccgtcctgc gccagcaatg
    12541 ttcctttctc aaacggcata aaattcagcg tgtcacttgc catatgcatt tcgaaggacg
    12601 gcgagtggcg agtaatttgc gaaaccaccc gataacggag cggcggcgtt ctcacgatac
    12661 cgacattctc accagatagc agcgcagcaa ttgcgctggc agtgactgca aactggcgaa
    12721 gatcgttttg tccaaagggc aacgctttgc cgagttccag cgtacaggcc agcgcgccaa
    12781 aatgtctggc gctaaaatgg gtaaacgtac caccaggcgt ctgatggaat accagtgcct
    12841 ccagccccgc cgcccccagc cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg
    12901 gtaatacacc aaactgcgga tgcaaggagc cacgaattgc ggtatggaga tcaaggtgcc
    12961 agcgcacaga ttctttgccc tggtcataaa aatcttccag gcactgttcc agttcgcgcg
    13021 cgcgacaggt ttccccgctt tcagcaaata gctgccaacg accgccaaac attcggttca
    13081 tatcgctatg gcaataacgt ttcccttgct tcagcgcagg aggattcccg aggatcacca
    13141 gcaaccgcca acgtaacggg atctcgccgt gagatatcgc gccaagcaac gcgtccagca
    13201 tctccacagg tgccgtctca ttaccgtgta ttcccgctga aatcaccagt gcgccttgcg
    13261 gtggcgttaa tggcgtcagt tccagcacac catcgcccag ccagagccag cgaacgccgt
    13321 tgatttctcg ctcggtgata accggttttt tacccgttaa ggtcagagca agaaaattat
    13381 ccattgcccc ctccctcgcg ctggaacgga taaaccgaac cgagattcag taattggctc
    13441 aatacatcca gtgcttcccg cccttcacgc agcaattgcg ggtcggccag atcggcagca
    13501 gtaagacgat cgcggtagta acgatccacc cagtcattga gcacattaaa cagcgtatcg
    13561 ttcatcatca ccgccggatt caccgcctgg cgttcttctt ctgtcaatac cacgcgcaac
    13621 cgcaggcacg ccgggcctcc gccattcgcc atgctttcac gtaaatcaaa gacttttagt
    13681 tcgctaatcg ggttgtcagc ggcaaggagt tcattgagat acccccatac tccggcgtgt
    13741 tcccgacact cctgaggcag caccaacatc atggaaccat catcgcggct cagcagttgg
    13801 ctgttaaaca gatatgtaga caccgcatct gacacggaaa cctgagtcgc cggaacttct
    13861 atcgccataa aaccgttcac ccgcgcacgc aggtttgcca gtaactgcac ctgtcgagcg
    13921 aacgcctgtt gatgacaaaa cagcacctgg cggttactca cggcaatcac gtcattatga
    13981 aaaacgccct ggtcgataac gtccgggttt tgctgggcga aaatcacctg ttggggattc
    14041 acctgattca gccttgccac cgcctcgctg gcttcgcgag tctgccgcgc cggataacgg
    14101 gaaggccggg tatcattgcc ttcttctcgc ccgtagacaa aaagttgcat acccggttca
    14161 ccgtaatgac cgccgagacg attgtggttt gccgccccct catcaccgag caaggctacc
    14221 tgcggcaacg ccgaatggac actaaatttc tcttcgtcgt taaaaatcgc ttttaacagc
    14281 gattcagtga cgggggcttc cagcgaacgg tgaaatttat tgttcaggtt ggcaacggtg
    14341 agatgcactt tgccatccag cgtatcggca gatggcgcga tcgttgccgc attggctacc
    14401 cacattggcg aagcggaact aacgctggaa agccagtgcg gtgcctggcg tgcaaccttt
    14461 tccagcactt gttcatcgct accactgaat cccaactgac gtaaaaccgg tataaacgga
    14521 cgctcgtgcg gcgggatcac ggcctggggg aatcccgcat cggcaagggt tttcattttc
    14581 agtaagccct gtttcgccgc cagtcgcggg ttagagacct gaaaacggtg acgggtagag
    14641 gcttcattac caaacgacag gcccgcgtaa tgatgcgtca gccctaccag cccgtcgaaa
    14701 ttgacttccc aggcgttcat cgcaccacct catcggaaaa atccaacccg gggttaagcg
    14761 tggcgggcaa tgttaacgag tccgactcca ggctcgccat cggccaggca caataatctg
    14821 cggcatacca ggcgctgggg cgatggttac cggaagcgcc aatgccgccg aatggcgcgg
    14881 tactggcagc gccggtaagc ggtttgttcc agttaacaat ccccgcccgc gcctcaagca
    14941 gcagttgatc gaatttttcc cgcgcggggg aaaccagacc gcatgagagg ccgaagcgag
    15001 tgttattcgc cattcgaatc gcttcatcga aattgtcata acgccagacg cgcaataacg
    15061 gtccgaacac ctcttcatct ggtacgccag caacgcccgt catttcaatg atccccggcg
    15121 tcagcaacga tgtccctgct tgtaataagc acggcgcaag caggggtcgt ccacccatcg
    15181 cttccagttg ctgccaggcc gtcaccacct gctgcgcggc ctgatctgat atcagcccgc
    15241 caataaacgg ctgcggttcg tcatcccatt tgcccggcgt taatcgctgg ctgacggcaa
    15301 ccagacgagc aagaaacgca tcgccctgcg ccccgctttt cagcaataaa cggcgggcgc
    15361 aggtgcagcg ttgcccggct g
//
LOCUS       DAYPHN010000114        15311 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000114 DAYPHN010000000
VERSION     DAYPHN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.114"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1044
                     /locus_tag="ACG0BW_003347"
     CDS             <1..1044
                     /locus_tag="ACG0BW_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFK7260272.1"
                     /translation="PRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDEAPEEEG
                     QVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVALGGCAAG
                     ALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQQADIDQ
                     SHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMIAVSPLA
                     LVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVREVGMDW
                     MSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRRQLIGQV
                     KETLQKGKTPSEK"
     gene            1192..2733
                     /gene="tyrR"
                     /locus_tag="ACG0BW_003348"
     CDS             1192..2733
                     /gene="tyrR"
                     /locus_tag="ACG0BW_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFK7260273.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDATTV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(2777..3283)
                     /gene="tpx"
                     /locus_tag="ACG0BW_003349"
     CDS             complement(2777..3283)
                     /gene="tpx"
                     /locus_tag="ACG0BW_003349"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFK7260274.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            3402..4367
                     /gene="ycjG"
                     /locus_tag="ACG0BW_003350"
     CDS             3402..4367
                     /gene="ycjG"
                     /locus_tag="ACG0BW_003350"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFK7260275.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLISITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(4342..5070)
                     /gene="mpaA"
                     /locus_tag="ACG0BW_003351"
     CDS             complement(4342..5070)
                     /gene="mpaA"
                     /locus_tag="ACG0BW_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFK7260276.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(5361..5999)
                     /locus_tag="ACG0BW_003352"
     CDS             complement(5361..5999)
                     /locus_tag="ACG0BW_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFK7260277.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(6070..6990)
                     /locus_tag="ACG0BW_003353"
     CDS             complement(6070..6990)
                     /locus_tag="ACG0BW_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFK7260278.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YTTLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            7128..8027
                     /gene="pgrR"
                     /locus_tag="ACG0BW_003354"
     CDS             7128..8027
                     /gene="pgrR"
                     /locus_tag="ACG0BW_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFK7260279.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            8364..9977
                     /gene="mppA"
                     /locus_tag="ACG0BW_003355"
     CDS             8364..9977
                     /gene="mppA"
                     /locus_tag="ACG0BW_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFK7260280.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTLVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGKLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLRGYPINNPEDVAYSRTMYIVKH"
     gene            complement(10028..11059)
                     /gene="ynaI"
                     /locus_tag="ACG0BW_003356"
     CDS             complement(10028..11059)
                     /gene="ynaI"
                     /locus_tag="ACG0BW_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFK7260281.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVSEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            11303..11560
                     /locus_tag="ACG0BW_003357"
     CDS             11303..11560
                     /locus_tag="ACG0BW_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFK7260282.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(11610..12560)
                     /gene="uspE"
                     /locus_tag="ACG0BW_003358"
     CDS             complement(11610..12560)
                     /gene="uspE"
                     /locus_tag="ACG0BW_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFK7260283.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTDWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(12712..13464)
                     /gene="fnr"
                     /locus_tag="ACG0BW_003359"
     CDS             complement(12712..13464)
                     /gene="fnr"
                     /locus_tag="ACG0BW_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFK7260284.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(13659..14174)
                     /gene="ogt"
                     /locus_tag="ACG0BW_003360"
     CDS             complement(13659..14174)
                     /gene="ogt"
                     /locus_tag="ACG0BW_003360"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFK7260285.1"
                     /translation="MLRLLEEKIVTPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(14185..>15311)
                     /locus_tag="ACG0BW_003361"
     CDS             complement(14185..>15311)
                     /locus_tag="ACG0BW_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFK7260286.1"
                     /translation="FFSHISSDAALVIMPPMGALIFLAVGRHPVAGLLAAIAGVGCGF
                     TANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMASSVVVLTIVGGLITDKII
                     EPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAIALMVIPENGILRDPINHT
                     VMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPHLMIEPMKEMAGFIVMVFP
                     LAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         3968 a         3585 c         3845 g         3913 t
ORIGIN      
        1 ccacgtattg atttcgacgg tccgctggag gtcgatcaga atccaaaatt cagggcgcag
       61 cagacctttg acgaaaatca ggcgcaaaat tttgccccgg ccacgctcga tgaagcgcct
      121 gaagaagagg ggcaagttga agcggtaatg gatgcagcgt tacgtccgaa acgcagcctg
      181 tggcgcaaaa tggtgatggg cgggctggct ctgtttggcg caagcgttgt cgggcagggt
      241 gtacagtgga caatgaatgc ctggcaaact caggactggg tggcgctggg tggatgtgcc
      301 gctggggcat tgattatcgg cgctggcgta ggttctgtgg taacagagtg gcggcgctta
      361 tggcgcttgc gacagcgcgc ccatgaacgc gacgaagcgc gcgatttgtt gcacagccac
      421 ggcacgggca aaggccgcgc attttgcgaa aaactggcgc agcaggcgga tattgatcag
      481 tctcatccag cgctgcaacg ctggtatgcc tcaatccatg aaacgcaaaa cgaccgtgaa
      541 gtggtcagtt tgtatgcgca tttggtccag ccagttttag atgcccaggc gcggcgcgaa
      601 atcagccgtt cggcggcgga atcaacgttg atgattgcgg tcagcccgct ggcgttggtc
      661 gatatggcgt ttatcgcctg gcgcaatctg cgtttgatca atcgcatcgc cacgctgtat
      721 ggcattgaac tgggatatta cagccgtttg cgcctgttta agctggtatt gctgaatatc
      781 gctttcgccg gagccagcga actggtgcgc gaagtgggga tggactggat gtcgcaagat
      841 ctcgctgcgc gtttgtctac ccgggcagct caggggattg gtgcaggact tctgacggca
      901 cgactgggga ttaaagctat ggagctttgc cgccctctgc cgtggattga cgatgacaaa
      961 cctcgcctcg gggatttccg tcgtcagctt atcggtcagg tgaaagagac gctgcaaaaa
     1021 ggcaaaacgc ccagcgaaaa ataatgcaat atcgggtgct gaccggatat ctttacgccg
     1081 aagtgcccgt ttttccgtct ttgtgtcaat gattgttgac agaaaccttc ctgctatcca
     1141 aatagtgtca tatcatcata ttaattgttc ttttttcagg tgaaggttcc catgcgtctg
     1201 gaagtctttt gtgaagaccg actcggtctg acccgcgaat tactcgatct actcgtgcta
     1261 agaggcattg atttacgcgg tattgagatt gaccccattg ggcgaatcta cctcaatttt
     1321 gctgaactgg agtttgagag tttcagcagt ctgatggctg aaatacgccg tattgcaggt
     1381 gttaccgatg tccgtactgt cccgtggatg ccttctgaac gtgagcatct ggcgttgagc
     1441 gcgttactgg aagcgttgcc tgaacctgtg ctctctgtcg atatgaaaag caaagtggat
     1501 atggcgaacc cggcgagctg tcagcttttt gggcaaaaat tggatcgcct gcgcaaccat
     1561 accgccgcgc aattgattaa cggctttaat tttttacgct ggctggaaag cgaaccgcag
     1621 gattcccata acgagcatgt cgttattaat ggacagaatt tcctgatgga gattacgcct
     1681 gtttatcttc aggatgaaaa tgatcaacac gtcctgaccg gtgcggtggt gatgttgcga
     1741 tcaacgattc gtatgggccg tcagttgcaa aatgtcgccg cccaggatgt cagcgccttt
     1801 agtcaaattg tcgccgtcag cccgaaaatg aagcatgttg tcgaacaggc gcagaaactg
     1861 gcgatgctaa gcgcgccatt gctgataacc ggtgataccg gcaccggaaa agatctcttt
     1921 gcgtatgcct gtcatcaggc aagcccacgc gcgggcaaac cctatctggc gctgaactgt
     1981 gcttctatcc cggaagatgc ggtcgagagt gaactgtttg gtcatgctcc ggaagggaag
     2041 aaagggttct ttgagcaggc gaacggtggt tcggtactgc tggatgaaat aggtgagatg
     2101 tcaccacgga tgcaggcgaa attactgcgt ttccttaatg atggcacttt ccgtcgggtt
     2161 ggcgaagacc atgaggtgca tgtcgatgtg cgggtgattt gcgcgacgca gaagaatctt
     2221 gtcgaactgg tgcaaaaagg cgtgttccgt gaagatctct attatcgtct gaacgtgttg
     2281 acgctcaatc tgccgccgct acgtgactgt ccgcaggaca tcatgccgtt aaccgagctg
     2341 ttcgtcgccc gctttgccga cgagcagggc gtgccgcgtc cgaaactggc cgctgacctg
     2401 aatactgtac ttacgcgtta tgcgtggccg ggaaatgtgc ggcagttaaa gaacgctatt
     2461 tatcgcgcac tgacacaact ggacggttat gagctgcgtc cacaggatat tttgttaccg
     2521 gattatgacg ccacaacggt agccgtgggc gaagatgcga tggaaggttc gctggacgaa
     2581 atcaccagcc gttttgaacg ctcggtatta acccagcttt atcgcaatta tcccagcacg
     2641 cgcaaactgg caaaacgcct gggcgtttcg cataccgcga ttgccaataa gttgcgggaa
     2701 tatggtctga gtcagaagaa gaacgaagag taagcgcgaa tatgcctgat ggtgcaacac
     2761 catcaggcat attaacttat gctttcagta cagccagagc tgcttcgtaa tccggctcgg
     2821 tggtgatttc atccaccagc tggctgaaaa tcacattgtc attttcatca ataaccacaa
     2881 cggcacgcgc tgccagacct ttcagtgggc catcagcaat tgccacaccg taggcttgca
     2941 ggaattcagc gttacggaaa gtggagaggg tgataacgtt gttcagacct tctgcgccgc
     3001 agaaacgaga ctgggcgaac ggcagatcgg cagagataca cagcacaacg gtgttgtcga
     3061 tctcggttgc cagttggtta aacttacgta ctgatgcggc gcaaacaccg gtatcaatac
     3121 tcgggaaaat gttcagcact ttgcgtttac ccgcaaactg accgagggtg acgtcagaca
     3181 gatcttttgc cacgagagta aaagtctgcg ctttgctacc cgcctgcggg atggaattgg
     3241 cgactgtaac cgggttgccc tggaaatgaa cggtttgtga catgattatc tttcctgttt
     3301 acatatagtt aacgtcacac ctagtttatg ctaactgtca ataacacagc aaacgctatt
     3361 tgcgcttaat ccgcagacca ccgcgacaac aaggagtaaa gatgagaacc gttaaggtat
     3421 tcgaggaagg ctggccctta cataccccgt ttgtgattgc tcggggaagt cgcagtgaag
     3481 cgcgcgtggt ggtggttgaa ctggaagaag aaggtattaa aggcaccggt gaatgcacgc
     3541 cgtatccgcg ttatggagaa agtgatgcct cggtaatggc gcaaattatg agcgtcgtgc
     3601 cgcaattaga gaaagggctg acacgggagg agttgcaaaa aattctccct gccggcgcag
     3661 cacgtaatgc gctggattgt gcattgtggg atctggcggc gcgaaaacag cagcaatcgc
     3721 tggctgattt gatcagcata acgcttcccg ggacagttat cactgcacag acggtagtca
     3781 tcggtacgcc tgatcagatg gccaatagtg catcaacact ctggcaggca ggcgcgaaat
     3841 tactgaaagt gaagctggat aaccatctta tcagtgagcg gatggtggca attcgcaccg
     3901 ctgtgcccga tgcgacgctg atcgttgatg caaatgaatc ctggcgtgca gaagggttgg
     3961 cggcgcgttg ccagctattg gcggatttag gcgttgcaat gcttgaacaa ccgcttcctg
     4021 cgcaggacga tgcggcgttg gagaatttta ttcatccgtt accgatttgt gctgatgaaa
     4081 gttgtcatac tcgtagcaat ctgaaggcgc tgaaagggcg ctatgagatg gttaacatta
     4141 agctcgataa aacggggggc ctgacggaag cgctggcgct ggcgactgaa gcacgtgcac
     4201 aaggttttag tctgatgctg ggctgcatgt tgtgtacctc tcgtgccatt agcgccgctt
     4261 taccgctggt gccgcaggtc agtttcgccg atcttgacgg accgacctgg ctggcggtag
     4321 atgtggaacc ggcgcttcag ttcacaacgg gcgaattgca tctttaggat gccagcgcag
     4381 caagtttgcc atcgcaaaga gatatttttc gctggcttcg tcggaggaga tgggtggaaa
     4441 ctctgcggtg atgcaatgca ggttgagatc cgcacaccaa ctgccgaaag agccaggcgt
     4501 ttcataaccg acgctggtta ccagaggcaa ttcaaacgcc tgggccagcc attcacctaa
     4561 ttcgctgtgt ctgggatctt caatacaggc cagcggatcg tggaatgaga ccacccaggc
     4621 gggttgaatg cggtgtataa gctggcacag cgcctgggtt tcaggttcag agccaggttt
     4681 gtcacctgtc agcaatacga catcacgttc ttcagcggcg ctgttccagc gataaacggt
     4741 ttcaccttcc ttccaattcg ccgccggaaa gtttctgttt aaatccacac cattagcatt
     4801 ggcccgtaac cccaactggc agccgtcagg attcacacac agcaccacat gatgacggcg
     4861 caaagaaggt gtcaacgtcc gcagagcaca ggagagcgtg acgaccgaag agttttcatc
     4921 accgtgagtg ccagccagaa tcaaaccact ttcgcggctg gctgcagggg caggaaacca
     4981 gattaacggc gcgcccaata atgaacgtcc gtaatgttct gttccgggcg gaaatgcgcc
     5041 gcgttcggcg cgtgggcggg ttacggtcat gatcgtctct ggttcaggaa gtattacagg
     5101 cagtgttgtg caaattttgc ggattatcaa atccttttct tcaacgctat gtcatcgatc
     5161 ttgtatcact tttttgccgg aagttgtttg cattttcatt gatgtgcaga tattagaatt
     5221 gtcgtaatgt tatatacatg gcgtatttta ttcttactgg ttctacacat gttaatcgca
     5281 tctgattgat cagttcacct taataagttt aataaatcgt actgcacata gtacagtacg
     5341 aaggtagcta tataattgat ttacatgaag aatggcttat cgccatcagt cccgggctga
     5401 ttgatgccta tattctcacc aatgtgtttc tcaggtttat cgatgatatc tgtaattaat
     5461 gctgcgacac tcttacgtga tacgatcgta cctttgaatg gttcattgcg tgaggtaagc
     5521 tcataatcaa taatatcttc atcggtcagc caggcagggc gaagaattgt atattccagt
     5581 ccagaagcct caattgcatc cgcagccctg cggaatggtt ttaggggctc accaataact
     5641 gcattattcc attcgccaaa ttttcctgga acttcatcat aaataccgag ggaaagaaca
     5701 aagatcagac gtttaacatc acaggctttc atcgcagcga tcactgaatt tgcctgaata
     5761 tctaaatctt cccccgtaag gttggcatac acaacatcct gtccctgcat ggcctgcttc
     5821 agtgccgcat gattcagtac atcccccata ataatcttgc tattagttgg atagggtttg
     5881 tggatttttg ctggctgacg ggcaaataaa gtttgtttaa tggtttgttt atctgccagt
     5941 tgattaatca catggcgggc tatctgaccg ccagcaccaa gaatcagtac atttttcatg
     6001 aaacttttcc cagattattg gcagcaatat gccaggccat atcactatta cggatttgaa
     6061 tatgtttcat tacagagttt cctcaaagaa cggcgctaat actgaaattg cttcagccac
     6121 atagactttg ccgtcataaa gatccatatg gtttgcgcct tcaacaatgt gatagcgttt
     6181 atcctggctt gatgctcgat cgtacaggtc gtcactcatc catttgctcc ctgcctggct
     6241 gcccgccaca atctgtatcg gctgagtcag gtacacttcc gccatatggt aagcatcata
     6301 ggtaataatc tggttaaggc tgcgcaaagt agtgtaaccc ggtgctgttg gatactgcgc
     6361 gcgaggggtg tggtaatact cccaggcctg acgcagttct tcgttcggcg catcggactc
     6421 cttcattggt gccagtggca tggtggcgta ttctccgctg ctaatatcgc tggttcgggc
     6481 gtttgaaccc gcgtcaacgt atggtagggc atcaatagat ttcacattgt tttcccaacc
     6541 attacggaac atcgaaccaa tattgaccgc actaacagta ccgatggcct tgatgcggcg
     6601 atcctgaatt gcagcattgg ctgtatatcc tgcaccggca caaattccca tcgcaccaat
     6661 tcggctattg tcgacataag aaagcgttgt cagataatca atcacggcac tgacgtcttc
     6721 agtacgaatg tatgggtttt ctaactgacg cggctcgccg ccgctttcac cttgataaga
     6781 tgcgtcataa gcaatagtga caaatccctt ttccgccagt ttttcggcat aggttccagc
     6841 cgtttgctct ttaacgcccc cacctgggtg agataccaca attgccggat actgacgggt
     6901 ttcatcaaat tttggaggga aatagatcac tgcagacaaa gagatggttg gattattgct
     6961 gttagtgaag ctgactttat tattcatcat tccgttcctc atggagttgt cggttcgttt
     7021 taacggttgg tgatatcact atagatattg atcattaagt tgattagacc caaaatcatg
     7081 attagaccta tcatttaaat gattaataga ttgtatggag atactgaatg aagcgtgaag
     7141 aaatcgctga tctgatggcg tttgtcgtcg ttgcagagga gcgtagcttc actcgcgcag
     7201 cagcccgcct gagcatggcg cagtcagctt taagccagat agtgcgccgt atagaagaac
     7261 gattgggatt gcggcttctg acacgaacca cgcgcagcgt tgtacccact gaagcgggcg
     7321 agcatctttt gtctgttctt ggccctatgt tgcatgacat agattctgcc ctgacatccc
     7381 tgagcgatct gcagaaccgc ccatccggga caatacgtat tactactgta gaacatgcag
     7441 caaaaacgat attgttacca gctatgcgca cattcctgaa atcgcatcct gaaattgata
     7501 tccagcttac cattgattat ggtttgaccg atgtcgtttc tgaacgtttt gatgcaggcg
     7561 tccgtctggg tggggagatg gataaagata tgatcgccat tcgaatcggg ccagatatac
     7621 caatggctat tgttggctca ccggattatt tttctcgtcg aagtgctcca acgtcagtgt
     7681 cacaattaat agatcatcag gcaattaatt tgtatcttcc cacatcgggt acagcaaatc
     7741 gctggagatt aatacgcggt ggacgtgaag ttcgtgttcg catggaaggt cagcttttac
     7801 tgaatacgat agacctgatc attgatgctg caattgatgg gcatggattg gcgtatctac
     7861 cttatgatca ggttgagcgg gctattaaag aaaaaaaact gatacgtgtt ttggataaat
     7921 tcacaccaga tttacccggt tatcacctgt actatccaca ccgtcgacat gctggctcgg
     7981 cattctcatt atttatagat aggctgaagt ataaaggtgc tgtttagcac tacttgctga
     8041 tacattaatt taattcttct cttaacgtat tctcagttcc tttcaacgtt ttggtcattt
     8101 tttattcttc gtacaatggc gacagatgct gattatgata ccgaaaacgg gtttgaacgt
     8161 gcgaagcccg aacgtagtgt tcggagttct atgtgcttta ccgcattttg gagactatta
     8221 tttacactaa atctgatttg atatattgat acttaaaaca tttgatgctt cctttgtcac
     8281 ttttttgatg gaagttgttt gcatttcttt aaggcgaaac aaataattac gcatcaattt
     8341 taatgtcggt tagagggaaa cttatgaagc actctgtttc agtcacgtgt tgtgcgctgt
     8401 tggtcagcag catttctctt tcgtatgctg cagaagttcc gagcggcaca gtactggcag
     8461 agaagcagga gctggtgcgc cacattaaag atgagcctgc gtcgctggat cccgctaaag
     8521 ccgtgggcct gccagagatt caggtcattc gcgatctgtt tgaaggactg gtgaatcaga
     8581 acgagaaagg ggagattgtc cccggcgttg cgactcagtg gaaaagtaat gacaaccgta
     8641 tctggacttt taccctgcgc gataacgcaa aatgggcgga tggcacactg gtaacggcgc
     8701 aagattttgt ctacagctgg caacgtctgg tggaccctaa aacgctatcg ccatttgcat
     8761 ggtttgccgc gctggcggga atcaacaacg cacaggcgat tattgatggt aaagctacgc
     8821 ctgaccagct tggcgtcacc gcagttgatg cccatacttt gaaaatccag cttgataaac
     8881 cgttgccgtg gtttgtgaat ttaaccgcta actttgcctt cttcccggtg caaaaagcca
     8941 acgtagaaag cagtaaagag tggacgaaac ccggaaaact gatcggtaat ggcgcttatg
     9001 ttcttaaaga gcgcgtagtc aatgaaaaac tggtcgtggt accgaatacc cattattggg
     9061 ataacgccaa aacggtgctg caaaaagtga ctttcctgcc aattaatcag gaatccgcag
     9121 ccactaagcg ttacctcgcg ggggatattg atatcaccga atccttcccg aaaaatatgt
     9181 atcagaagct gttgaaggat attccggggc aggtttatac gccgccgcag ctcgggacct
     9241 attattatgc gtttaacacg caaaaagggc cgacggcaga tcagcgcgtt cgcctggcat
     9301 taagtatgac gatagatcgc cgcctgatga ccgaaaaagt gttggggacg ggcgaaaagc
     9361 cagcgtggca ttttacacca gatgttaccg cgggatttac gccggaacct tcgccgtttg
     9421 aacaaatgag tcaggaagaa ctgaatgcgc aggcaaaaac tttgttgagc gccgctggtt
     9481 atggtccgca aaaaccgctg aagctgacgc ttttgtataa cacttcagaa aaccatcaaa
     9541 aaattgcgat tgctgtagca tcaatgtgga aaaagaacct tggcgtagat gttaaattgc
     9601 aaaatcagga atggaaaacc tatatcgata gccgtaacac cggcaatttt gatgttatcc
     9661 gagcctcgtg ggtaggggat tataatgaac cctccacttt cctgacatta ttaacgtcaa
     9721 cgcattcagg aaatatttca cgctttaaca atccggcata cgacaaagta ctggcccagg
     9781 catcaacgga aaataccgtt aaagcgcgta atgccgatta caacgcggca gaaaaaatcc
     9841 tcatggagca agcaccgatt gcaccaattt atcaatatac caatggacga ttaatcaagc
     9901 cgtggctgag aggttatccc attaataacc cggaagatgt ggcgtacagt cggactatgt
     9961 atattgtgaa gcattgatgt gatgggaatt ggcgttaccc ttgtggataa cgccagtgat
    10021 gtctgattta gcggccctgg tccggcggtg taatgttatc catatacagc gtctggctgg
    10081 ggaaggcaaa gtccgcgccg tgtgactgta caatatcgat aatcttcaaa taaacgtctt
    10141 gctgtgcagc aagccattca gcccataccg tggttttggt aaagcaataa accataatat
    10201 tcaatgagga gtcagcaaac tggttgaaat aaaccagtaa ggtttgtcgc tggtcaatgg
    10261 ccgggtgatt tttcagcatc tcacgtacgg cttcgacaat aacgcccact tttgccgcat
    10321 cctcataacg taaaccaatg gtcgtggtaa tgcgccggtt ggtcattcgt cctgggtttt
    10381 ctacgctgat tgacgaaaac agcgagttcg gtacgtacaa cggacgatta tcaaaagtcg
    10441 taatcttggt aattcgccag ccaatttccg atactgtacc ttcgatattt ctgtccggtg
    10501 aacggatcca gtcgccaata ctaaaaggac ggtcgaaata gagcataatc ccggaaaaga
    10561 agttactcag aatatcttta ccggccatac cgacagccag accaccaata ccaccaaagg
    10621 tcaacaagcc agaaaggctc atgccgaaat gttcgccata aagcagaaca agcaccacaa
    10681 taatggtgat tttgatgata cgcgacataa tccgcgcact ggtgatatcg cgaccttttt
    10741 taatctgctg tttttcaaac tgattaatca gcagaaatag cttaatcgtc agaataaccg
    10801 caatcaggga cgtacagata aaatcgataa cgcctggggt gataaatttg agtttatagt
    10861 tttctataac ataattaatg atgctaccaa cagcactgat aattatggtg tagattaaaa
    10921 attgcaccgc atggaataaa aaaccttttc ttttacgatt tccgcggcga aaccaaaagc
    10981 tcatcagaat caatgctgcg cagctaccga aaataatgac cagattaagc gcattatttg
    11041 taaacagttc agcgatcatt gttttatcag gctcctccag ataattgtcg tcatgccgga
    11101 aacccctggc ggggctattt taccgcgaca attcattgag atcatcaata gtcagggaag
    11161 gaagtagcaa cattagctaa ctaatcaatg cgttgattgt aaatccagct aagaggtgag
    11221 gttttcagag cagacaacgg tgaaatgtca tggtattgtt acgtttaggt aacaagaaat
    11281 ttgtctgcac aaggattaca tcatgattat ggcgaaactg aagtcagcga aagggaagaa
    11341 atttctcttt ggtttgttgg cggttttcat tattgcggcg tcggttgtga ctcgcgcgac
    11401 catcggcggc gtgatagaac agtacaatat tccgctgtct gagtggacga catcaatgta
    11461 tgtgattcag tcatcaatga tttttgttta tagcctggtc tttactgtgt tgctggcaat
    11521 cccgttggga atttatttcc ttggcggcga agagcagtaa gtaaaaaata ggcccgataa
    11581 ctcgggcctt gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc cagttcaacg
    11641 ggtgtctgat actggtcagg tttaatgacc agcaggtcgc agcgaagatg atcaatcacc
    11701 tgttctgccg tgttgccgag gaatgctgct gaaataccgg tgcgtcctac cgtgcccaga
    11761 accacaatcc ccgcctgtaa gtgctccgcc aaatcaggaa tcacctcttc tggcagacct
    11821 ttttctacgt gcgtcatgtt ttcattaatg ccgaatttct gccgcagggc tttcattgcc
    11881 agcaaatgtt gcccacgaat ggcatcgtta taaacgctcg gatcaaattc cggcagttca
    11941 atcgcgatat taattggcgt tacgggataa gcgccaacca gatgaacttc ggtatggttg
    12001 acttgttccg ccagttcgat cgtctctttg accagttttt cattgagcgc attatggtac
    12061 ggctcttcac tggcgagatt caccgccacc agcgccttac ccccttccgg ccacggctgg
    12121 tctttcacca tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc cgttggcgta
    12181 aaaatcaccg cttccagacg gtcatgttgg tgcgccattt ttagcaccaa atcgtgcccg
    12241 ccgctgatca cttcctgaat gatggcttcg aaaggacggt tatgccagac cactttaatt
    12301 tcaatgggaa cgccagcatt gagataatat tttgcctgct cgtggatcca gtctgtacgc
    12361 tggctgatga cgccctgacg catagcggta cgttcgtccg gagagagcag ggtggtcatt
    12421 tcgtatgaga agtcatagat cggcaaaaag gctttaattt tgccaccaat ccgttgatgt
    12481 aaataaacag ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat aacaacgagc
    12541 atgttctgat acatagccat acagggtctc cttacaacaa ctgtcaacgc agtttgtaat
    12601 taaaagatta acccatatct ggtgaatgaa acagtgatga accttctgcc agatcaataa
    12661 atcagaaaaa tttaatgata tgacagaagg atagtgagtt atgcggaaaa atcaggcaac
    12721 gttacgcgta tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg tgatgtattt
    12781 acctttgact gccagcatac cgcttttctg gaagcgaccc agcagacggc tgatggtttc
    12841 taccgtcagg cccagatagt taccgatatc gccacgagtc atcgtcaggc ggaattcacg
    12901 aggggagaag ccgcgttggg caaaacgacg ggacaggttg tagatgaatg cagccagacg
    12961 ttcctcggca tttttcttcg acaacagcag gatcatgtcc tgatcgcctt tgatttcacc
    13021 gctcatcaga cgcatcatct gctgacgcag attcggcatt ttaccagaca aatcgtccag
    13081 cgtttcgaac gggatttcac ataccatcga ggtttccagc gcctgcgcga agctcgggtg
    13141 atgaccgctg ccgatagcgt caaatcccac cagatcgccc gctaaatgga aaccagtgat
    13201 ttgctcgtcg ccttgctcag tgatggtata acttttaatc gtaccggagc ggatggcata
    13261 aagcgattta agttcatctc cagccttaaa cagcgtctgg cctttctgaa taggcttctt
    13321 ccgctcaatg atattatcaa gctgatcaag ctcatgttcg ttgagtgtga acgggatgca
    13381 aagctggctg atgctgcaat cctggcaatg gatagcacaa ccgccagact gaatgcgccg
    13441 tataattcgc ttttccggga tcataggtct gctcaagccg taattgatat ttgtcaattt
    13501 taacatcttt ttagagagca agtaagtcta agcaaacctt aacagcagag aattccgata
    13561 ttagatgtaa atatatgtct atctatttga aaacccttaa gttgttaagg gtaactttac
    13621 ataaaagtgt gaacaagctg gcacaaattg tttaatgttt acagcaaaag ataaccttcg
    13681 tggcgtaata accactcttt tcgctgaact ccgcctgcat atccggtcat ggtaccgttt
    13741 ctgccaatga cccgatgaca aggcacaaca atgctgatgg gattcgatcc gtttgccgca
    13801 ccaacggcac gcgccgcacc agggcggccc aattgttcag ccagttggcc gtaatgcatt
    13861 acctgcccgc aggggattgt gcgtaatgtt ttccagactt cgcgctgaaa tggcgtcccc
    13921 cccgtggcag tgggaagcgt atcaataatg ctaagattac cggcaaaata ttcacgaagc
    13981 ttgtcgctta aaccacctgg gttggtggca gaaatgcgtt catagccttc tttgcggtaa
    14041 tggatgtcca gcagctgcac catgcgttcg ctgtactctt cccattcaac cgcccgcagg
    14101 cgaaattgct catcgcaaat cacccacagt ggacccagtg gcgtgacaat tttttcttca
    14161 agtaatctca gcatccgttc tctcttaaga caaacgtggg taaatccccg gacctatcgg
    14221 cagaccgaca agataccacg ccagcaacat cagcagccat accaccaaaa agataagtgg
    14281 atagggcaaa actaacgaat agtaagtacc cagtttcgcg tctggtttgt aacgttgcag
    14341 gaatccaaga aacagtggaa caaaaggaga taccggcgct aaaggcaata cggatgagtc
    14401 ggcaatacga aagaggattt gcgcaaatgc cgggtgaaag ccaagtagca taaacatcgg
    14461 tacgaaaatg ggggccagaa tcgaccagat tgcggaaccg ctggcgataa acatgcataa
    14521 gaaagaggaa agcaacgcca gaccgacaaa cgccgggatg ccgctaagcc ctgaactttc
    14581 cagtatatcg gtcagcccca cggcgatgaa tttccccatg ttgctccagt taaacatggc
    14641 gacaaactgg gcgaggggaa aaaccatcac gataaatccc gccatctctt tcatcggttc
    14701 aatcattaaa tgcggtaaat ccgcctgacg tcgaattgtg cgggtagcga tgccataagc
    14761 cagcgagacc acaaagaaaa aaagaatgat cagtggcacg atacctttaa taaaggggga
    14821 tggcatcacg gtgtgattaa tcggatcgcg caatatcccg ttttccggga tcaccatcag
    14881 cgcaatcgca gcaataaaaa gtagcgatac gacacctgct atgcgtaaac caaaacgctg
    14941 actttcggtt aatgtctgca gtttctcatc gctgtttccc tgccattgac ctaaacgtgg
    15001 ctcaatgatt ttgtcggtta tcaggccgcc aacaatcgtc agtacgacta cggagctggc
    15061 cataaaatac cagttatcaa ttacactgac gtgcatttgc ggattgaacg cagccgcagc
    15121 ctccgtgctg atccccgaca gcaacacgtc ggttgtgaca atcagtaaat tagccgtaaa
    15181 gccgcaacct acgcctgcaa tggcggccag taaacctgca actggatgcc tgcccaccgc
    15241 cagaaaaatc agcgcaccca tcggtggcat gatcactaac gccgcatcgg aagaaatgtg
    15301 gctaaaaaaa g
//
LOCUS       DAYPHN010000115        15195 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000115 DAYPHN010000000
VERSION     DAYPHN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.115"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            105..1082
                     /gene="ybeQ"
                     /locus_tag="ACG0BW_003362"
     CDS             105..1082
                     /gene="ybeQ"
                     /locus_tag="ACG0BW_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFK7260287.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCDAQYIIGFYYNRDSAIDSP
                     DDEKAFYWLRLAAEQGHCEAQYSLGQKYTEDKSRHKDNGQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQASGTRDVSQEIREIEDLL"
     gene            complement(1153..1635)
                     /gene="ybeL"
                     /locus_tag="ACG0BW_003363"
     CDS             complement(1153..1635)
                     /gene="ybeL"
                     /locus_tag="ACG0BW_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFK7260288.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1870..4452
                     /gene="leuS"
                     /locus_tag="ACG0BW_003364"
     CDS             1870..4452
                     /gene="leuS"
                     /locus_tag="ACG0BW_003364"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFK7260289.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKDLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4467..5048
                     /gene="lptE"
                     /locus_tag="ACG0BW_003365"
     CDS             4467..5048
                     /gene="lptE"
                     /locus_tag="ACG0BW_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFK7260290.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5048..6079
                     /gene="holA"
                     /locus_tag="ACG0BW_003366"
     CDS             5048..6079
                     /gene="holA"
                     /locus_tag="ACG0BW_003366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFK7260291.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6081..6722
                     /gene="nadD"
                     /locus_tag="ACG0BW_003367"
     CDS             6081..6722
                     /gene="nadD"
                     /locus_tag="ACG0BW_003367"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFK7260292.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQWPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6746..7357
                     /gene="cobC"
                     /locus_tag="ACG0BW_003368"
     CDS             6746..7357
                     /gene="cobC"
                     /locus_tag="ACG0BW_003368"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFK7260293.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7617..7934
                     /gene="rsfS"
                     /locus_tag="ACG0BW_003369"
     CDS             7617..7934
                     /gene="rsfS"
                     /locus_tag="ACG0BW_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFK7260294.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7938..8405
                     /gene="rlmH"
                     /locus_tag="ACG0BW_003370"
     CDS             7938..8405
                     /gene="rlmH"
                     /locus_tag="ACG0BW_003370"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFK7260295.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8436..10337
                     /gene="mrdA"
                     /locus_tag="ACG0BW_003371"
     CDS             8436..10337
                     /gene="mrdA"
                     /locus_tag="ACG0BW_003371"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFK7260296.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10340..11452
                     /gene="mrdB"
                     /locus_tag="ACG0BW_003372"
                     /gene_synonym="rodA"
     CDS             10340..11452
                     /gene="mrdB"
                     /locus_tag="ACG0BW_003372"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFK7260297.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11463..12551
                     /gene="rlpA"
                     /locus_tag="ACG0BW_003373"
     CDS             11463..12551
                     /gene="rlpA"
                     /locus_tag="ACG0BW_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFK7260298.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12691..13902
                     /gene="dacA"
                     /locus_tag="ACG0BW_003374"
     CDS             12691..13902
                     /gene="dacA"
                     /locus_tag="ACG0BW_003374"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFK7260299.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14012..14275
                     /gene="ybeD"
                     /locus_tag="ACG0BW_003375"
     CDS             14012..14275
                     /gene="ybeD"
                     /locus_tag="ACG0BW_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFK7260300.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14376..15017
                     /gene="lipB"
                     /locus_tag="ACG0BW_003376"
     CDS             14376..15017
                     /gene="lipB"
                     /locus_tag="ACG0BW_003376"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFK7260301.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3766 a         3862 c         3960 g         3607 t
ORIGIN      
        1 ttatactttt ctgacgcaac ataatacgca taaaataagg cttacgcaaa aatggaacac
       61 ataaaaatat aatatgcttt atatgcaacc ataaggaaat gataatgatt tttacgtcaa
      121 gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg
      181 atgctcaata tattattggg ttttattata atcgcgatag cgcaattgac tctccggacg
      241 acgaaaaggc cttttactgg ctgaggctgg ccgctgagca aggtcattgt gaagcacaat
      301 attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat gggcaagcca
      361 tcttttggct gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct
      421 ggacactgga tcgtggagaa gaccccaact ataaagaagc ggttgcctgg tatcagatag
      481 ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca
      541 acggagtcgc acaagactat acgctggcat ttttctggta caaacaagct gcattacaag
      601 gccatagtga cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc
      661 aaaacgagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcatg
      721 cccagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc
      781 aagccatgta ctggtatctg aaggctgccg ctcaggggag cgtcggcgct tacgtcaaca
      841 tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggcc gcctttgaat
      901 ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt
      961 atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag
     1021 ttcaggcatc cggtaccagg gatgtcagtc aagaaatccg tgagattgaa gatttactgt
     1081 aagagataaa aacaagggcc ggggaatttt attcggccca tattctcttt attgctgtgc
     1141 gaaatcatac cattaaggct cgaacgggcg tctctggaac tggtcatgac cacatttcgg
     1201 gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aaatgacatt tctcgcagac
     1261 cagattcccc agccctacca cttctccgct gtgataaacc ccgtgatgat tgaggtcctg
     1321 gaaaacctcg cgccattcaa gctgcgtttt atcggtgatg tctgccagct cctgccacaa
     1381 gctttcttta atcacccgca taaagacgct gtcagactct tctttcaggc tctcttcata
     1441 gctcatggcg aactcttcca ggtcacgtct gacagctcgc gtcagctcat cgacctcggt
     1501 tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat
     1561 atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac ggtaatattg
     1621 agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc
     1681 tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat gaataatccg
     1741 ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc
     1801 tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa aacaggacca
     1861 ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat
     1921 tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc
     1981 ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc
     2041 atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct gcagccgatc
     2101 ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgcaccg
     2161 gcaccgtgga cgtacgacaa catcgcgtac atgaaaaacc agctcaaaat gctgggcttt
     2221 ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag
     2281 aaattcttca ccgagctgta taaaaaagac ctggtatata agaagacttc tgcggtcaac
     2341 tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg
     2401 cgctgcgata ccaaagttga gcgtaaagag atcccgcaat ggtttatcaa aatcaccgct
     2461 tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga caccgttaaa
     2521 accatgcagc gtaactggat tggtcgttcc gaaggcgtag aaatcacttt caacgttaac
     2581 gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc
     2641 tacctggcgg tagcggcagg tcatccgctg gcacagaaag cggcggaaaa taatcctgaa
     2701 ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga aatggcaacg
     2761 atggagaaaa aaggcgtcga tactggcttt aaagcggttc acccattaac gggcgaagaa
     2821 attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agttatggcg
     2881 gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct gaacatcaaa
     2941 ccggttatcc tggcagctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa
     3001 aaaggcgttc tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac
     3061 gctatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg
     3121 cgcgactggg gtgtttcccg tcaacgttac tggggcgcgc cgattccgat ggtgacgctg
     3181 gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat
     3241 gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc aaaaactacc
     3301 gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc
     3361 tggtactatg cgcgctacac ttgcccggag tacaaagaag gtatgctgga ttccaaagcg
     3421 gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac
     3481 ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac
     3541 gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt
     3601 ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag
     3661 aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg
     3721 agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac
     3781 ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa
     3841 tggcaggaat ctggcgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtt
     3901 tacgagcaca cagcgaaagg tgatgttgcg gcactgaacg ttgatgcgct gactgaagat
     3961 cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga tgatatcggc
     4021 cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg
     4081 aaagcaccga ccgatggcga gcaggaccgc gccctgatgc aggaagcgct gctggcggta
     4141 gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa
     4201 ggcgaaggcg atatcgacaa cgcaccgtgg ccggttgctg acgaaaaagc gatggtggaa
     4261 gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgcaaaaat caccgttccg
     4321 gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa
     4381 tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac ccggtaaact cctcaatctg
     4441 gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg
     4501 cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag gttccttcca
     4561 ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc
     4621 gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg
     4681 ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg gtattccgta
     4741 acggtcaaac agcagagtat cagatgatca tgacggtcaa tgcgaccgtg ttgatccccg
     4801 gccgtgacat ctacccgatt agcgccaaag tcttccgttc gttcttcgat aacccgcaaa
     4861 tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg
     4921 ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag
     4981 aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctct accacgctgg
     5041 gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg
     5101 cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctat
     5161 tcgtcaggtc gctgcggcgc aagggttcga agaacaccac actttttcca ttgatcccaa
     5221 cactgactgg aatgcgatct tttcgttatg ccaggccatg agtctgtttg ccagtcgaca
     5281 aacgctattg ctgttgttac ccgaaaacgg accgaatgcg gcgatcaatg aacaacttct
     5341 cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag
     5401 caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac
     5461 ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgtg caaaacagct
     5521 caacttagaa ctggatgacg ctgcaaacca agtgctctgc tactgttatg aaggtaacct
     5581 gctggcgctg gcccaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac
     5641 attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt ttcattgggt
     5701 tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc aacttcgtct
     5761 ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactgt tgttactggt
     5821 taacctgaaa cgccagtctg cccatacgcc actgcgtgca ttgtttgata agcatcgggt
     5881 atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagcc agccgcagtt
     5941 acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacggtca
     6001 gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac ctctggcgga
     6061 cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc
     6121 cggtgcacta tggacatctt aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc
     6181 gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg
     6241 tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg
     6301 aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc
     6361 aggaacaatg gccagacgtg ccgctggcgt ttattattgg tcaggattca ctgctgacct
     6421 ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc
     6481 gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt
     6541 tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa
     6601 cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcgt
     6661 gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct
     6721 gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacgca
     6781 agcgaatatc gatggtctgt acagcggtca tgctcccaca cccctgactg cgcgcggtat
     6841 tgagcaagcg caaaatctgc atacgctgct acatggtgtt tcctttgacc tggttttatg
     6901 cagtgaactg gaacgggcac agcataccgc gcgacgggtt ctcagtgatc gccagcttcc
     6961 cgtgcaaatc atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca
     7021 tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca
     7081 tgctatcccc acgaacggtg aaggatttca ggcattttca caacgagtgg aacggtttat
     7141 cgcaaggctt agtgaatttc agcactatca gaatatttta gtcgtcagcc atcagggtgt
     7201 actgagtctg ttaatcgccc gtttaattgg tatgcctgcc gaagctatgt ggcattttcg
     7261 cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga cgctacgcgt
     7321 cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa
     7381 gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca gacttttttc
     7441 gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg
     7501 gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct
     7561 ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc
     7621 agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca
     7681 tcatcgcctt agacgttcag ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg
     7741 gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag
     7801 cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt
     7861 tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa
     7921 aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact
     7981 gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc
     8041 tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca
     8101 aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc
     8161 caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg
     8221 gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg
     8281 cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgttg gttcgcgtgc
     8341 tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg
     8401 agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg
     8461 actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt
     8521 tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg
     8581 attaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg
     8641 gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag
     8701 aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg
     8761 ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt
     8821 tcacctccat tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca
     8881 atcagtaccg ttttccgggt gttgaagtta aaggctataa acgtcgttac tatccttacg
     8941 gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac
     9001 gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg
     9061 gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag
     9121 ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt gccaccgcaa gccggacacg
     9181 atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta
     9241 gccgcgcagc ggtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca
     9301 cgcctagtta tgacccaaac ctatttgttg acggtatctc cagcaaagat tattccgcct
     9361 tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg
     9421 cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggagtg atcacgcgca
     9481 atacgacgct gtttgaccca ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg
     9541 actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg
     9601 ataccttctt ctatcaggtg gcctacgata tgggaatcga tcgcctctcc gaatggatgg
     9661 gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt tccggcaaca
     9721 tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca
     9781 ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac
     9841 tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg
     9901 aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc gatattcatt
     9961 ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta
    10021 cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc
    10081 aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac
    10141 gtgaccacaa actgatgacc gcctttgcgc catacaataa tccgcaagtg gctgtcgcca
    10201 tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg
    10261 accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca gcggttgccg
    10321 cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt
    10381 ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt
    10441 tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc
    10501 gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg
    10561 ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc
    10621 catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga
    10681 aattgccaaa atagccgtgc cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc
    10741 gccatcgttg aagaacacag gcatcgcgct ggtgctgata tttatgccca cgctgctggt
    10801 ggctgcgcag cctgacctgg ggacatcaat cctcgttgcg ctttccggtc tgtttgtact
    10861 gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt
    10921 cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct
    10981 cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc
    11041 tattggctcc ggcggattac gcggcaaagg ctggctgcat ggcactcagt cacaacttga
    11101 atttctcccc gaacgccata ccgactttat cttcgcggta ctggcggaag agctgggatt
    11161 agtgggcatt ttgattctgc tcgctcttta cattctgctg atcatgcgcg ggctgtggat
    11221 agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt
    11281 attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt
    11341 tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg
    11401 gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg
    11461 caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa
    11521 gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt aacggcccca
    11581 tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg
    11641 attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttagccagg
    11701 cgggactggc ggcaatctat gatgccgaac ccggcagtaa cctgacggcc tctggcgaag
    11761 ctttcgatcc gacacagctg acggcggccc atccaacgct tccgatcccc agctacgcca
    11821 gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggtcctt
    11881 acggcaatga ccgcgttatt tcgctttctc gcgcagcagc tgaccgtctt aacacgtcaa
    11941 acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc
    12001 ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt
    12061 taagcggcgg cgcaggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca
    12121 gtaattcgac gctaaaaagc gaagatccga ctggcgcgcc ggtaaccagc agcggtttcc
    12181 tcggcgcacc aacgacctta gcgcctggtg ttctggaagg cagcgaacca acgcctgcac
    12241 cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggttacac
    12301 cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg gccgttagcg
    12361 atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc
    12421 gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc agcaaagccg
    12481 aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacca
    12541 ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa
    12601 gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg cacttttttt
    12661 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    12721 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    12781 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    12841 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    12901 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    12961 aaatttaaag aaactgattt agtcaccatc ggcaacgacg catgggccac cggtaacccg
    13021 gtatttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    13081 ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    13141 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgccctgggc
    13201 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    13261 gcgcgagata tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg
    13321 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggtctg
    13381 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    13441 tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatctctgc ggtgatgggc
    13501 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctgacctg gggcttccgt
    13561 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    13621 tttggtgatt ctgatcgcgc ttcgttagga gttgataaag acgtgtacct gaccattccg
    13681 cgtggccgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    13741 ccgctgcaaa agaatcaggt cgtcggtact atcaacttcc agcttgatgg caaaacgatc
    13801 gaacaacgcc cgctggttgt gctgcaagaa atcccggaag gcaacttctt cggcaaaatc
    13861 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaatcgaa cacttgaaag
    13921 tgtaatttcc gtccccatat actaaacatc agtaaaaaac tcccgccttc tggcgggagt
    13981 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    14041 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    14101 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    14161 cagcaaaggc aactaccact cggtatctat cactatcaac gcgactcata tcgagcaggt
    14221 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    14281 ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    14341 ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
    14401 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
    14461 cccgcgatga aagtaccctt gatgaaatct ggctggtcga gcactatccg gtatttaccc
    14521 aagggcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg gtgattcaga
    14581 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtgttgc
    14641 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    14701 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg
    14761 tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
    14821 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    14881 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    14941 ataatattgc accgcgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
    15001 aatatattac cgcttaattg catacatcaa tggcccaatt tacactgggt cattactcat
    15061 aacacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
    15121 aatgcgatgc tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg
    15181 attatggtga gcaaa
//
LOCUS       DAYPHN010000116        15130 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000116 DAYPHN010000000
VERSION     DAYPHN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.116"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            176..904
                     /gene="gadW"
                     /locus_tag="ACG0BW_003377"
     CDS             176..904
                     /gene="gadW"
                     /locus_tag="ACG0BW_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFK7260302.1"
                     /translation="MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1267..4380)
                     /gene="mdtF"
                     /locus_tag="ACG0BW_003378"
     CDS             complement(1267..4380)
                     /gene="mdtF"
                     /locus_tag="ACG0BW_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFK7260303.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTNSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4405..5514)
                     /gene="mdtE"
                     /locus_tag="ACG0BW_003379"
     CDS             complement(4405..5514)
                     /gene="mdtE"
                     /locus_tag="ACG0BW_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFK7260304.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGTLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5622..5900
                     /locus_tag="ACG0BW_003380"
     CDS             5622..5900
                     /locus_tag="ACG0BW_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260305.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5901..6428)
                     /gene="gadE"
                     /locus_tag="ACG0BW_003381"
     CDS             complement(5901..6428)
                     /gene="gadE"
                     /locus_tag="ACG0BW_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFK7260306.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7225..7797)
                     /gene="hdeD"
                     /locus_tag="ACG0BW_003382"
     CDS             complement(7225..7797)
                     /gene="hdeD"
                     /locus_tag="ACG0BW_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFK7260307.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGVALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            8052..8384
                     /gene="hdeA"
                     /locus_tag="ACG0BW_003383"
     CDS             8052..8384
                     /gene="hdeA"
                     /locus_tag="ACG0BW_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFK7260308.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8500..8826
                     /gene="hdeB"
                     /locus_tag="ACG0BW_003384"
     CDS             8500..8826
                     /gene="hdeB"
                     /locus_tag="ACG0BW_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFK7260309.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8890..9537
                     /gene="yhiD"
                     /locus_tag="ACG0BW_003385"
     CDS             8890..9537
                     /gene="yhiD"
                     /locus_tag="ACG0BW_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFK7260310.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQKKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9589..10359)
                     /locus_tag="ACG0BW_003386"
     CDS             complement(9589..10359)
                     /locus_tag="ACG0BW_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFK7260311.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(10356..11348)
                     /gene="shuU"
                     /locus_tag="ACG0BW_003387"
                     /gene_synonym="chuU"
     CDS             complement(10356..11348)
                     /gene="shuU"
                     /locus_tag="ACG0BW_003387"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFK7260312.1"
                     /translation="MLKDSLSSARIFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALAAAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVALVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLSGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(11397..12020)
                     /gene="chuY"
                     /locus_tag="ACG0BW_003388"
     CDS             complement(11397..12020)
                     /gene="chuY"
                     /locus_tag="ACG0BW_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFK7260313.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVHRADVAAHIHELANAPALNQQVHSLIEPDLKPA"
     gene            complement(12020..12514)
                     /gene="hutX"
                     /locus_tag="ACG0BW_003389"
     CDS             complement(12020..12514)
                     /gene="hutX"
                     /locus_tag="ACG0BW_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFK7260314.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVNAFHALAASL
                     KEHA"
     gene            complement(12527..13864)
                     /gene="hutW"
                     /locus_tag="ACG0BW_003390"
     CDS             complement(12527..13864)
                     /gene="hutW"
                     /locus_tag="ACG0BW_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFK7260315.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLIYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDGCLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(13884..14798)
                     /gene="shuT"
                     /locus_tag="ACG0BW_003391"
     CDS             complement(13884..14798)
                     /gene="shuT"
                     /locus_tag="ACG0BW_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFK7260316.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         3952 a         3629 c         3655 g         3894 t
ORIGIN      
        1 aaatatattc gtgttgcatt tacttattat caattaactg ttatgcaaaa ctactttgtg
       61 gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt ctggcatcat
      121 ttcattagta tactgaaatt gaaataatcg cagtaatgaa atataaggga tagtcatgac
      181 tcatgtctgc tcggtgatcc tcgttcgtcg ttcattcgat atttatcatg aacagcaaaa
      241 aatatcgttg cacaacgaga gtatcctgtt actggataaa aatttggcag acgattttgc
      301 gttttgttca ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt
      361 acaaaatatt cgtcagatac cacgcaattt agggttacat agcaaagacc gtctgttaat
      421 taaccagtca ccccccatgc cgctggtgac ggcgatcttt gatagtttca atgactccgg
      481 ggtaaattcg ccgatactga gcaaaatgct ctatctttcc tgtttatcga tgttttctca
      541 taagaaagaa ctgatcccct tacttttcaa tagtatcagt actgtttcag gaaaagttga
      601 acgccttatt agctttgata tcgctaaacg ttggtatctg cgcgatatcg cggaaagaat
      661 gtataccagc gagagtctaa tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa
      721 aatattactc gcctccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc
      781 tctgcatact attgcggaaa aatgtggcta tagcagtaca tcgtacttta taaacacatt
      841 tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc
      901 ctgacatatt ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct
      961 tccctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat
     1021 aggtattttc aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac
     1081 gattacggga atgtgggtca ctattgtgtt attcaaattg atgacgaatg gctggagtgg
     1141 tttctacttt cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag
     1201 tggtgaatgg ttggcaggaa agagtaaggc tgaaccctca cgttcaacct tactctcatt
     1261 tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg
     1321 aacgaagtaa attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac
     1381 cgcgttttgc gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat
     1441 aaaggccaga gaggtcatca ggattgggcg taaacgcatt cgcgccgctt cgatgatcgc
     1501 ctctatcggc gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat
     1561 ggcgtttttg gcggaaagcc cgatggtggt cagcaaacca acctggaagt agacgtcatt
     1621 acttaagccg cgtagatcgg tggccagtaa tgcgccaacg acgcctaacg gtacaaccaa
     1681 catcaccgag aacggaattg accagctctc ataaagtgcg gcgagggcga ggaacaccac
     1741 gaccagtgaa atggcataca gcgcgggagc ctgatttgag gataacgctt cctgatacga
     1801 cagtccggtc catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa
     1861 tttcatggcg tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg
     1921 gatgccgttg tagcgttcca gtcgcggtga accataggtc cattcggtag acgaataggc
     1981 cgaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg
     2041 caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag
     2101 gaagtcgttc acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag
     2161 cgccacaccc attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc
     2221 ttccagtccg ttcggacgta ccccggtgac ttgattcggt gattgcgctg ccagtgataa
     2281 tagctcgttt cgcgcctggg tcagtttttc gtgccccagg ttgccgttgt ccagcagttc
     2341 catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac
     2401 gacggcttta ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga
     2461 gttttcctca ccgacacgtt cagaccacgg tttgaggctg ataaacgcca gaccgttgtt
     2521 ttgcccctgg ccgctgaagc caaagccgcc aacggtaaac accgactgaa cattatcttt
     2581 ctctttagtc agataataat ccgtcacctg ttgcagcact ttcgtggtgt taaccatggt
     2641 ggcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa
     2701 agaggtcggc gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac
     2761 catgtagcga ccggtacaac gcaacagcga gcgggtgcta ttggtatagt gctgagttga
     2821 tttttcaaac agcgtgttga agcgtgcgaa cagggcgtta ggtttatgac cgccttccgg
     2881 cgcggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga
     2941 aagcagcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcacc
     3001 gctcataaag gccatcggca taaacactgc ggaaagaaca acggcaatcc cgaccagcgc
     3061 acgttggatc tgccccatcg atttatgcgt tgcttccttc ggcggtagct tatcttccgc
     3121 aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc
     3181 cagcaccatc ccgaacatag ttaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc
     3241 aaacgtcccg agaataacca ccggtacggc aatcgtcggg atgattgtgg cacggaaatt
     3301 ctgcaaaaac agatacatga ccaggaagac gaggatgata gcctcaacca gtgttttaaa
     3361 aacttcctga atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag
     3421 acttgcgggg aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc
     3481 cagggcgttt gctccggtag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata
     3541 gcgcgccacg gtggaataat cttccgctcc aagttcgacg cgggcgacat cacgcagcag
     3601 cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg
     3661 cagacgcgtc tgcacaatga tcgaggcgtt tagctgctgg tctgccgcct gtggcatgcc
     3721 acccaattgc ccaccagaaa tctggttgtt ctgcacctta atctgggaaa taacatcgga
     3781 aggtaccaga ttgtatttat tgagtttttg cgggtccagc cagatacgca tggcgtactc
     3841 ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt
     3901 agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc
     3961 cgctaccatc aggatattac tgctcgactt atcgacgcta atcccctgct gctgcaccgc
     4021 ttcaggtaat gaaggcatag cgagctgcag tttattttgc acctgaacct gtgcgatatc
     4081 aggagatgtc ccagtctcga aggtcagagt aatagaggca ttacccgccg catcactggt
     4141 tgaagacatg tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac
     4201 cgagtcttct accgtttgcg catcggcacc tggataggta gcgctgatgg taatggtcgg
     4261 tggcgcaatc tgcggatact gcgcaaccgg taagttcatg atcgccagac cgcctgcaag
     4321 catcataata atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat
     4381 gaaagtcccc ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct
     4441 ggaggaaatt gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacaatcac
     4501 ccgatcgcca gcctgtaagc cagaggtgac gacccactgg tcgccgatgg ctttgctggc
     4561 ttcaatttcg cgtagctgca caacatcgtc tttatccaga atgagcgccg ttgctttacc
     4621 ctgggcgttg tgggtgacac cttcctgcgg caccagtaat acattctggc ggctaccttc
     4681 atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt tggggaaaat
     4741 cgcccgtaac gtcacggagc ccgtggtttc atccaccgtc gggtcggaga atttcagcgt
     4801 gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg tactgccctg
     4861 aacctgtttg atttgcccac tggcgacctc ttctttcata cgcaagaaat cctgcaccga
     4921 ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg aatctgcctg
     4981 attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg
     5041 cgaggtgaca tttgcgtatt gcaggttgat agtcgcctgt tcaacagccg ctttggcgac
     5101 ggtgacattg gcttctgctt cattcaactg ggtgcgcgcg gtgtcgtaat cctgacggct
     5161 aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg cattgctggc
     5221 ggtagagagc gctttcgcca gcgtgccttt ggcggagttc agctcggcct gtaaaggtgc
     5281 aggatcaatc tgatacagcg aatcgccctg gttcacttta tcgccttcga taaagttgcg
     5341 tttaatgata ataccgccca cctggggacg tatctcggca acttcataag gaacggttct
     5401 accgggtaat tcgctcaaca cattgaccga accgggggag agcgtgacga caccgacctc
     5461 aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga gcatcgcgcc
     5521 gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc ctgaaaattc
     5581 ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat ttacagacca
     5641 gcggcatagt ggggttgtca gccagtaagg tgggatacag gcatagtgat cgacatggtg
     5701 aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc attcgggcaa
     5761 cgaagggttc tactggtgga tacacatacc aggggaataa agtcgttttc gcctctcctt
     5821 tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc agccatcaat
     5881 ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc
     5941 atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa tgtttgttgc
     6001 gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat cagttgcata
     6061 attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa gtgtttacca
     6121 taagtaatct caagaagatt tttgatactt tctttgcggc tgacaaaata aataggcgct
     6181 ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc ggcatctaat
     6241 ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt atcaatgata
     6301 accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat ttttatcatt
     6361 tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga
     6421 aaaatcataa cttgctcctt agccgttatc gtttcgaaca tgtcatcctt ttcattattt
     6481 acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt tgcccttcgt
     6541 gaactaatac gtttattaac actaatcaat acttcagatt tatccatcac gcactccata
     6601 tattgatcct aagtaagatt cctggttgtt aacagtttgt aaatagtaaa caaaaagtta
     6661 acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga tctctgaaga
     6721 atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt ttcgacttaa
     6781 gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag taaataactc
     6841 attcatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg caatgtaatc
     6901 aacgcctgtt gcaaaaaatg aacccggatt acttatttaa ccgcccatag catcaacgct
     6961 tacggaactc tttgttcact ttcccacagc acttttaaaa cattaaacct gactacggaa
     7021 aatatcagcc atgataatgt ttgaaatggc taatttgcca tggaatgaaa aaatcagatg
     7081 aaattcagta ggttgaaata atcatcagca ggtaattatt tcaatgatag tgcgcaattg
     7141 atctacaacg ctgtgtagcg gagagagtat taatcggatc atagtcacat caagtgacta
     7201 tgatccgggt gacaactggg ataattattg ctgcttaacg aacaaactgg cgaagctgaa
     7261 caggctggcg gcgctaaata tcagttcaat acccaccagt gtggaaacca aagttacaga
     7321 caccatcggc gttgcaccaa ggaatatcca ggcaatgacg atatccagca caccaataac
     7381 gagctgtagc cagctgcctt tcattgaacg ctgacgatac caactcatca ggcgaataac
     7441 ccctgcaaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc ccagctccgg
     7501 tgcgcggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga aaccggataa
     7561 taccggccag aaattatgac tgcggttgct gaataatcca acaataagcg caacgcccga
     7621 gcatattaat aatgcaccca ctaccgtgct taaaatatcg ccagagacga acgggaaact
     7681 gatacacagc aacccgacga taaacagcaa cacggcaata aactggattg ctctgcgatg
     7741 ctttttcagc atctccagat caaacttcaa aattgttgcc ttatctatat ataacataga
     7801 accaccctat aaaattaaga agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct
     7861 aagaatgcag tcgatttaat aaaaatttcc taattacagt atctgatgta tctgtaactc
     7921 attgtattga aataaaaata tctgattttg atattttcca tcaacatgac atatacagaa
     7981 aaccaggtta taacctcagt gtcgaaattg attcgtgacg gctctttcac tttatagttg
     8041 aggatattac gatgaaaaaa gtattaggcg ttattcttgg tggtctgctt cttctgccag
     8101 ttgtgagcaa tgcagcggat gcgcaaaaag cagctgataa caaaaaaccg gtcaactcct
     8161 ggacctgtga agatttcctg gctgtggacg aatccttcca gccaactgca gttggttttg
     8221 ctgaagcgct gaacaacaaa gataaaccag aagatgcggt tttagatgtt cagggtattg
     8281 caaccgtaac cccagctatc gttcaggctt gtactcagga taaacaagcc aactttaaag
     8341 ataaagttaa aggcgaatgg gacaaaatta agaaagatat gtaattccgg gaatgcgtta
     8401 catcgtactt ccttgcatat tgaacaggcc ggaatatctt ctttaaaagc agctattcct
     8461 cctgttcata tataatctct atattgaatg ggttacaaaa tgaatatttc atctctccgt
     8521 aaagcgttta tttttatggg cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg
     8581 ctggcagcca atgaatccgc taaagatatg acctgccagg aatttattga tctgaatcca
     8641 aaagccatga caccggttgc atggtggatg ctgcatgaag aaacagtata taaaggtggc
     8701 gataccgtta ctttaaatga aaccgatctc actcaaattc ctaaagtgat cgaatactgc
     8761 aagaaaaacc cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg
     8821 aattaatgag gtacaagtaa aaaggagtag caagttgagc catcttgctg ctcctttttg
     8881 catttttata tgacagcaga atttattata cgtcttatac tcgcagcaat tgcctgtggc
     8941 gctattggca tggaaaggca aatgcgcggc aaaggagcag ggttacgcac acatgtatta
     9001 attggcatgg gaagcgccct gtttatgatt gtttcgaaat atggttttgc tgacgtactg
     9061 tctttagatc acgtcggact cgaccccagc cgtattgctg ctcaggtggt gacgggcgtc
     9121 gggtttatcg gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg tctgacgacg
     9181 gcagcagata tctgggtgac cgccgccatt ggtatggtta ttggtagcgg tatgtacgaa
     9241 ctgggcattt atggttcagt gatgacctta ctggtgctgg aagtcttcca tcaattaacc
     9301 ttccgcctga tgaacaaaaa ttaccattta cagctgacat tagtgaacgg taatacggta
     9361 tcaatgctcg actggtttaa gcagaaaaaa ataaaaacgg atttagtttc gttgcaggaa
     9421 aatgaagatc atgaagtggt cgccattgat atccagcttc atgccacgac gtcgatagaa
     9481 gatctgttgc gattattaaa aggtatggca ggggtgaaag gcgtttcaat tagctaatct
     9541 gtatagttat ttattcacag gagtaccaga gcggtactcc tgttggtttt aatgatcgag
     9601 gacgataagg ggtgaatggt tggcaggatc tttaagtacg gtaatatccg cgccgtagag
     9661 cattgtcagc gcctgctgtg tcaatacgtc ctgcggttta ccatttgcga taacctttcc
     9721 tttttgcatt aatacaatac ggtctgcata acgcgcggca agatttagat cgtgcaatac
     9781 gcagcaaaca ttgaactgcc gttcgtgaac tagctggcgt aataaacgaa acaagtgctg
     9841 ctgatggtga atatccagcg ccgaagttgg ttcatcaaga aataaccatt tcggtgaggg
     9901 agtaggttcc cagagttgca ccagcaggcg cgctaattgc acgcgctgtt gttcaccacc
     9961 ggataattgc cgatagtcgc ggtttgctaa cgcctgacag tcacataacg ccattatttg
    10021 tgctgtttca ttatcctgat taccagtacg atgcggatgc cgccccatct ggataacttc
    10081 ttgtacgctg aatggaaacg ccatgtggct attttgacgc atcacggccc ggtgtttcgc
    10141 cagttcagta atcgaccatt catttaatgg cttattaaac aaccgacatt cgccgctatc
    10201 aggttgaaga tagccggtta actggcgaag cagcgtagat tttcccgcac cattagggcc
    10261 gagaatggca acaatttccc caccgggaaa agtcagcgag acattatcag tcagacgccg
    10321 cccctgaagg ctataaacca gattctgggc gctgatcatg tgatcctccg gttgcgcaga
    10381 atcaaccaca taaaataagg accaccaagc aggctggtta ataatcccac gggcatttct
    10441 gctggctgta ccagcgtgcg ggcaagcgtg tctgccatca gcaataaaca ggctccggcg
    10501 agggcggagc aagggattag ccagcggtga tttgccccgg tagtcatacg aatcagatgc
    10561 gggatcacca ggccaataaa gccgataatg ccgcttaccg atacggcggc accaacgagc
    10621 agtgagctca ctaacagtaa ttgctggcgt ttacgcttaa cgttcacgcc gaggtagtgg
    10681 gcttcttcat caccgagctg cagtaaattc agcgttccgg cgagacaagt tgttgcgata
    10741 atggccggta ggatgaatga actggcaacc aataacgtcg gccattgcgc ctgtccaaga
    10801 ttgcccatca tccacaatgt gagttgtcgt agctgctgct catcgccgat atagctcagt
    10861 atgccgatcg ctgcgccgca cagggcattg atggcgatac cggagagtaa taattgcatc
    10921 atgctgccat cgcgatggcg ctgcgtgatg agaaaaatga tggtgcagac cactaaactt
    10981 ccggcaatgg caaacaccat ttgctcatag agcaccagcg ccacggggaa ggagaagggc
    11041 agcacaatag caacgccgac cattaatgca gaaccgctac tgacgccgag taatccaggg
    11101 tcagccattg ggttgcgaaa aagcccctgc ataattaccc cggcggctgc caacgctgca
    11161 cctaccagca ccgccagcaa aacccgtggt agacgaatat tgagccagat atcgcggtat
    11221 tcctcatcaa aaagggcatc aaaactgatc tttaacgctc cctgactggc accgaacagc
    11281 accagagcga gcaataatag tgatagcccc ataaagatcc tggcggaaga gagtgaatcc
    11341 ttgagcacgc aaaactccgt tacgacatta cggcgcggag attccgcgcc ggggtatcac
    11401 gccggtttca ggtccggttc aatcagactg tggacctgct ggttaagtgc tggcgcattt
    11461 gccagttcat ggatatgtgc ggcgacatcg gcacggtgaa caaaaccatg acactcctga
    11521 ttttgtatcc gttgtgcttt gcccgttgcc gccccatcaa gcagaccgcc agggcggaga
    11581 atggcgtaat caagctggct ggtttgcaac cagctttccg ccagcgtttt ttcccgtacc
    11641 gcctgaccaa acgcagcctt tgcccgctcc gataaaaacg gccagctatc gccgcatccc
    11701 aaagaggtca ccagaatcat gcgactgatg ccggcctttt cggcctcatc aatcactgtt
    11761 ctgtgcgcca gataatcctg agctcctccc attgttgaga tgataagcgc atccgggcca
    11821 gctgcgcggc aagcagccgc aactacgctg gcatcacagg cgtcgcctgt aaaaacctgt
    11881 acgccttgtt gcgccagttt cgtggcggca tcggcatgac gaatgacagc aacgaccgga
    11941 cgttgttccg ccagcgccag ttcaagcgtt ctggcaccca caccttttcc gcctgcgcca
    12001 aagagtagcc acggcgtcat caggcgtgtt cctttaacga tgccgccaga gcatggaaag
    12061 cgttgacctg ttcgctcagg agctgacgat ggtcatccct gccgaggaag attttcagca
    12121 tggcgctgcc ttccttattg aaaaagagaa tagaggcggt gtccatgccc ataaatttac
    12181 gttcgatcaa ggcaatatgc gtgcagtttt ctgctttgat atgcccggac atgccgtgct
    12241 ttccgcgtag gttgaaatag ccgtggcggt ggaaacccga aggcagttcg ccgctaaatt
    12301 cgaggattac atcggcagta tgtactaacg tggtgacgtt gccccattcg cagacggtat
    12361 cccagacagt atcgaattta tcgcctgaca ctatggtaga ggagggtaga tttctcacta
    12421 cctccagcag tgtggtgttg tattgttcgg ctaccacctc cagcgtaccg tccggctccg
    12481 ttttcaaaaa ttcttgtaac gagacatggc tcataaatca ctcctgttat ggtttttcac
    12541 gcacaatcgc tggcgcatta agtacctgaa ttagttcatt aagagactgc aaaatattgc
    12601 ttgcccagaa acgaccttca ttagtcagcc gcaggcaagt tgacgcatcg cggcttaagc
    12661 ctttttggtg ccattgagcc agtaacggcg cgagtttttc ggcatgtggc gtaagttcat
    12721 ccagcggtac acgcgctgtt tcaacacctg actgcaaggt atgacgccat tgcgcattac
    12781 gttcggcgtt acgcatgatc atcatcagcg gttttttgcc tgccgcgacg gattcatgcc
    12841 aggtctgcag attgcgttcg ttcatccagg agtaaccatt aatcgaaccg ccggctccgg
    12901 aaccaaaggc cagacaatcg gcaccttgtt ttatcagcag gttatagaga ttgcgttcgc
    12961 gtgtggtacg gccccagtgg ctgttactga tgcagcgcca accggcatcg tccataaaat
    13021 cacacccttg cagataaaga tcgcgacgtt ctgccggtga gggtacggta gtgcgcccat
    13081 tttccacggc tttgcccagc ggtgtattgg gcaggacatt gagcgcatag agatcgacgc
    13141 cgtcgagacc gatatcgcga gcaatagcca gatcttctcc ccaggtttgc gcatcctgac
    13201 ccggcaggcc aaacagcagg tcacagacca ccgcagcacg gtcacgtttg accaggcttt
    13261 ccataaacgc aatggcggtt gggccatccg aggtgcgggc cattttcttg cggattttgc
    13321 tgttaaacga ctggatacca attgagaagc ggtttgctcc tgcatcaaga caaccatcga
    13381 ttcgctcagc gtcaaaattc agtacccggc cttcgatagt gatttcacaa tcaggggcca
    13441 gtggcagctt ttctcgtaat gtggtgatga ttctggccaa atcgtgtgcc gaaagcgcgg
    13501 aaggcgtacc gccaccgaaa tagaccgcgt ggatgggggc agactgatgc aatacgctgt
    13561 ccgcttccat ttctatttca cgaatcaggg cgtcggtata atgagcacac gcatcttcat
    13621 taaaacggtt ctgataaaag ccacaaaacg tgcagtgtgt cgcacaaaaa ggaatgtgta
    13681 ggtaaatcag tcgcttacga ggtgatgccg tttgattgat catctcttgc caggtctgtg
    13741 ccagttgctc tttggcaacc ggaatggctc cacgaaacgg catcatggcg cgcctgtctt
    13801 taaatggctg atcaccgtct aaagcaaaat gtggcgtgag atccagggtg ttatttgtgt
    13861 tcattcgtct gatttaaatc catttattgc ggccaaagct gctggtgtaa agactcaacg
    13921 acatcagcaa tacgcggacc cattcccaga atcaggtttt gatcaacggt gataatgcgc
    13981 tggtttttcc atgcggcagt gtgggtaatt ccggcaatag aacgtagccg attaatatcg
    14041 ccatcgacca tttgtgaggt tacgacaatc acttctggat tggccgcaat cagtgattct
    14101 gcactgtagc ttttgtactg ctggtgggtt gcgacgtttt ctgctccggc aagcgaaaga
    14161 atggcgtccg cgacgctgcc tttaccggca acctgtggcg cacttccgcc tgcggagaga
    14221 ataaacatgg ctttaaccgg ggcttttttg gccgccacgt tttgctgtac tcgctccagg
    14281 cgttggttga tctgtgtcac cagcgcctcg ccttgttcag gaacctgtag cgtttttgcc
    14341 agttggcgaa tgttggcgta catctgctga agggtggcag gcacacgcgg cagggtgacg
    14401 acgttgactt tctgcgctcg tagttggtcg agcacaattt gtggcccggc atcctgccag
    14461 gtaattacgc tatccgggcg aagtgacaaa atgccttcgc tgctgagttg tttccagtag
    14521 ccaatatgcg gcagtttggc ggtttctggt ggataagatg tcgtttcatc gacaccaacc
    14581 acgcgttcgc cagcgcccat cgcgtagatt agctccgtca gcgatcctcc tgcgaccacg
    14641 atacgttcgg cagccgttac gctaaaagcc cagctaagag ccagtactgc cgtaaacaat
    14701 ttttttcgtt ttaccgcggt cttcgcggca gaggccacgg ctgaaataca taacgtcagt
    14761 ggggaaaaaa gaaacggcct ggtgatgatc cttggcataa tatgagaact cttatcaaat
    14821 tgagaatgat catgattatc atttgagtta tatatgaaat acaaccgtcg attcaatacg
    14881 caaggcgtta caagcgtatt tatttttgat ttttatcaag atgcttcgtc agctaacgtt
    14941 cttgttaccg actaaatttc agcgcgggaa aaagctgaga acccagagat atcgaggctt
    15001 gcagagcaaa gcgaagagaa aattatcact gttaacgaca aagtttagtg atgaggtcat
    15061 gggggaataa ccataaaaat aaagtggttt ccagggcgca ggataagttt cataaccccc
    15121 tgtttattgt
//
LOCUS       DAYPHN010000117        15112 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000117 DAYPHN010000000
VERSION     DAYPHN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.117"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..511)
                     /locus_tag="ACG0BW_003392"
     CDS             complement(<1..511)
                     /locus_tag="ACG0BW_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFK7260317.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMEN"
     gene            636..1544
                     /gene="mak"
                     /locus_tag="ACG0BW_003393"
     CDS             636..1544
                     /gene="mak"
                     /locus_tag="ACG0BW_003393"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFK7260318.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(1687..2871)
                     /gene="araJ"
                     /locus_tag="ACG0BW_003394"
     CDS             complement(1687..2871)
                     /gene="araJ"
                     /locus_tag="ACG0BW_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFK7260319.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFFCCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     VG"
     gene            complement(2997..6140)
                     /gene="sbcC"
                     /locus_tag="ACG0BW_003395"
     CDS             complement(2997..6140)
                     /gene="sbcC"
                     /locus_tag="ACG0BW_003395"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFK7260320.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVTIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNCIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALQSQQQTLQQQE
                     ALAAQSLQKAQAQFDTALQDSIFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQA
                     LAIQTAETLTQHQQHRPGGLALTVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESVFAVK"
     gene            complement(6137..7339)
                     /gene="sbcD"
                     /locus_tag="ACG0BW_003396"
     CDS             complement(6137..7339)
                     /gene="sbcD"
                     /locus_tag="ACG0BW_003396"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFK7260321.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7529..8218
                     /gene="phoB"
                     /locus_tag="ACG0BW_003397"
     CDS             7529..8218
                     /gene="phoB"
                     /locus_tag="ACG0BW_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFK7260322.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            8276..9571
                     /gene="phoR"
                     /locus_tag="ACG0BW_003398"
     CDS             8276..9571
                     /gene="phoR"
                     /locus_tag="ACG0BW_003398"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFK7260323.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGVFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            9978..11297
                     /gene="brnQ"
                     /locus_tag="ACG0BW_003399"
     CDS             9978..11297
                     /gene="brnQ"
                     /locus_tag="ACG0BW_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFK7260324.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            11373..12746
                     /gene="proY"
                     /locus_tag="ACG0BW_003400"
     CDS             11373..12746
                     /gene="proY"
                     /locus_tag="ACG0BW_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFK7260325.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            12902..14719
                     /gene="malZ"
                     /locus_tag="ACG0BW_003401"
     CDS             12902..14719
                     /gene="malZ"
                     /locus_tag="ACG0BW_003401"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFK7260326.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQLGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQHIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTDGILALPAISATVWIN"
     gene            14908..>15112
                     /locus_tag="ACG0BW_003402"
     CDS             14908..>15112
                     /locus_tag="ACG0BW_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260327.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSPHGKLLFI
                     SGNENLVNISAEKIDEIKNSKDFF"
BASE COUNT         3320 a         3838 c         4101 g         3853 t
ORIGIN      
        1 ggttttccat gctcaatggt acaaccggta acgaccccag gcttttacgc agtaatgcca
       61 gcgtatcttc cgcttttttg gcactggcgc agtccaccat aatcaaaccg ttaaccgtgt
      121 cgatccacat cattgtctgg ctaaaacggc tgaaagcacg tggcagcaga gagtgcagca
      181 cttcgtcttt cagcgaatct ttttcggttt tcttcagctt acgcgcctgt tccgcttcca
      241 gtttggcgat tttcgcttcc agcgcctgtt taatcaccgg agacgggagg attttttctt
      301 ctttgcgcgc gcagataaca atttgaccat tggcaacgtg cgttaacgca tcgctgtgcg
      361 atcccatcgg aggaacccag cccatcttcg ccatgtcctg gctgccgcat ggggtaaatg
      421 ccatcgaggc tagctgtttt tccatctctt ctgcacgcag cgaaatctcg cggctaagac
      481 ggtaaaccat taaatttttg aaccacagca tgataatttc cacggccttg tcgttaaatt
      541 tagcgggcat gataacgaat tgtcggcggc cttgcattgc caatccggtt gtccgtctct
      601 acgctattga tattgaaaaa aataaggaga gtaccgtgcg tataggtatc gatttaggcg
      661 gcaccaaaac tgaagtgatt gcactgggcg atgcagggga gcagttgtac cgccatcgtc
      721 tgcccacgcc gcgtgatgat taccgccaga ccattgaaac gatcgccacg ctggttgata
      781 tggcggagca ggcgacaggg cagcgcggaa cagtagggat gggcattcct ggctcaattt
      841 cgccttacac cggcgtggtg aagaatgcca attcaacctg gctcaacggt cagccattcg
      901 ataaagactt aagcgcgcgg ttgcagcggg aagtgcggct ggcaaatgac gccaactgtc
      961 tggcggtttc agaagcagta gatggcgcgg cagcgggagc gcagacggta tttgccgtga
     1021 ttatcggcac gggatgcggc gcgggcgtgg cattcaatgg gcgggcgcat atcggcggca
     1081 atggcacggc aggtgagtgg ggacacaatc cgctaccgtg gatggacgaa gacgaactgc
     1141 gttatcgcga ggaagtccct tgttattgcg gtaaacaagg ttgtattgaa acctttattt
     1201 cgggcacggg attcgcgacg gattatcgtc gtttgagcgg acatgcgctg aaaggcagtg
     1261 aaattatccg cctggttgaa gaaagcgatc cggtagcgga actggcattg cgtcgctacg
     1321 agctgcggct ggcaaaatcg ctggcacatg tcgtgaatat tctcgatccg gatgtgattg
     1381 tcctgggggg cgggatgagc aatgtagacc gtttatatca aacggttccg cagttgatta
     1441 aacaatttgt cttcggcggc gaatgtgaaa cgccggtgcg taaggcgaag cacggtgatt
     1501 ccagcggcgt acgcggcgct gcatggttat ggccacaaga gtaaaaaacg taggcaattg
     1561 gctcatcatg cctgatgcga cgcttgccgc gtcttatcag gcctgcaaaa tgtgccagaa
     1621 ccgcgtaggg cggataaggc gttcacgccg catccggcaa taagcaacgt gctccgtgac
     1681 tgcaatctac cccactggtt tcgccagcac cggactatcc gccgcttgct ggcgcttata
     1741 gcgaccatac agcagcaacg acgacatcgc agcaaacgaa agcagggcgg caggtagcgc
     1801 cacgtaatta tatgccagcc ccagcgtcag catcatacca ccgcaatatg cgccgacggc
     1861 gctaccgagg ttaaacgcta tttgcccacc tgcggcacct aataactctc cgcctttggc
     1921 attttgcagt aacaatattt gcagtggtgc tgaaagggca aataatcccg cgcaacaaaa
     1981 aaatgcaaaa ataagcgacg ttgttttcat gccgccgaag aaaaagagca tcagcagtgc
     2041 cagcacaata ataaagtcag ttactgccgc aatgcgcagt ggtgaataac gtcctgaaat
     2101 ccggccactt agcacattcc ccagcaccat ccctagccca actaacatca taataaaagt
     2161 catcgccgtt tccgaaaaac cggaaataaa catcatgtat ggctttacgt agctgaacca
     2221 ggcaaataca cctgcgttgc caaacatggt ggcggcgaaa attaaccacg gggccgggct
     2281 gcgcaaaaag tgaaattgtt cgcgtagctt ccctttcgcc tcgtcgcgaa tatccggcac
     2341 ccaaaaatag accgatgcca tcaccgcaat attaaaaaca gcgatcaata aaaaggtgta
     2401 acgccagcta aattcctgac ttaaatacgt tcccagcgga atgcccagca aattggcgac
     2461 tgtcatcccg gaaaccatcc ccgccacggc ggcggtgact ttgccgggtt tgataatttt
     2521 cgataacacg atcgcgccga cgccaaaaaa tgcaccatgc ggaaagccgg aaaccagccg
     2581 accaatggcg agcatcaggt aagacgaaga gagcgtgaac atggcgttgc caatgacgca
     2641 caacgccacc agaaacaata agatatgttt gagtgagtag cggctggaaa agagtgcgat
     2701 gattggcgca ccgaccacca cccccagcgc ataatacgag atcatatgcc cggcagcagg
     2761 aattgaaatt cctacgttat gcgccagctc tgtgagcacg cccataatgc caaattcggc
     2821 catccccaaa ccaaacgtgc ccagagccaa agataaaata acttttttca taccaccatc
     2881 acaaccacca gaacggctac taatcaccgt agccaataaa gtgatagctt gcaggataac
     2941 gaccagcgag gccaagagat aattcgcccc tctgtattca ttatcctgct gaatagttat
     3001 ttcactgcaa acacactttc cagtttgctg tagcccagtc cattgatctt cttcactttg
     3061 atctgcaccg gaatacgctc tttcatcgct tcaacgtggc taatcacacc gatggttttg
     3121 ccgctggcat tcagggcatc cagcgcatca agggcggtat ccagcgtttc actatccagt
     3181 gtgccaaaac cttcatcaag gaacagcgag tcaatacgcg ttttatggct gaccagatcc
     3241 gaaagcgcca gcgccagcgc cagactgacg aggaaacttt cgccgccaga aagggtacgg
     3301 gtgtcgcgta ccgcatctgc ctgccaggta tcaacaacct cgacttccag cgcctcgctg
     3361 gctttgcgct gtaacagata gcgcccgtgc agccgggtaa gttgctgatt agcgagatgg
     3421 actaaattat ccagcgtcag cccctgggca aacttgcgga atttatcgcc ctctttggaa
     3481 cctattagcg aattcagata tccccagtcc tcaacctgct gcgtcatttg agcaatttgc
     3541 tgcagtaagg tttgttgttg ctgacggtta tctgcatcct gcttcagctg ctggcgaatc
     3601 tcgccctgac tggtggtgtt ttcacgcaac ttttgctgag tttgcgctaa ctcttgctga
     3661 atctgctcca ccgttacagt gagagccaac ccgccaggtc ggtgttgctg atgctgtgtc
     3721 agcgtttctg ctgtctgaat ggccaacgct tgcgcctgac gacgctggtt ttccagattc
     3781 tgtttcagct gttccagctg cgttagcgtc tgctcatcca ttaacgccgc aagaaatgcc
     3841 tgctgatcgt caaaaatgct gtcctgtagc gcggtgtcaa actgcgcctg ggctttttgc
     3901 agactttgcg ctgccagagc ctcctgttgc tgcaacgttt gctgctggct ttgcaacgcg
     3961 aggcattgtt cgtgaacttg ccgccagttt tccagcgcta cagtttcttc gctgtgcggg
     4021 agttcatcac tttgcggcaa cgtttccaga atcggcgtca gttgctgaat acagttttgc
     4081 agcgcggtta attcgttctg gcgatgctgc cagctctgcg cttcttgctg acgcgtcgcc
     4141 aaccagctct cttcttcatc ttcctgtggc aatgtcagcg cataacccgc caatgccgtt
     4201 aagagctgct gctggcgttg ttcaatttgc tgttgatact ggataatttg ctgattatgc
     4261 gcggcaatct gcccttgtaa ttcatgccgt tggctgagta accgcagctg gcgttcgtgc
     4321 tcatcttgtg catccagcca cggttgaata tcgtcctgtg gctgcaaggt gatattgagg
     4381 ctggccgtga cggcttgcca ttgttgagta agtgcttgct catcttgtcg gaggctttgc
     4441 gcttcgtttt catcacgctg aagttgcttt gttaaggcgt ccagttgccc acgtagcgtc
     4501 gcaccttctt caccgagctt tttaacttcg ttttccagcg ccagtaatcg agactgatta
     4561 acgccaggct ccagcgcctg atacgcctcg accgccgggt ggctggtgga accacaaagt
     4621 ggacaaggct gacccgcctg taactgtgca cgttgagctt ccagcgtttt gatgcgcgct
     4681 tcctgctcgc aaatggtttt cacatcggca agttgctgcg tcttttcttt ataacgctgg
     4741 cgcatttcgt taagtgcggc gttacgttgc gtctgttctt gcgtgacatt ctggatagtg
     4801 acctgtaatt gcgccaggcg tttttgttgg ggaacaatct gtccatgcag cgcgaccagg
     4861 cgctgacgca gtgggcgttg ctcagcatgt tgcgccaggg cgctagcaac ttcatcggcg
     4921 gttaacgtca acgtgatcgc cgcaagcgca ttaagttttt gctctgcatg ggttaactgt
     4981 tgctgccatt gccgcagatg ctcgcgatcg ctggtttgtt gggagaactg cgcacgccaa
     5041 cccgccagtt cgttgttcca ctgacggaag cggtcgtgtt cctgtaacca ggtattcagg
     5101 ctttgttgct gctgctgtaa ttctgctgac tgcttcgccg cgtggtggcg aatgctcgcg
     5161 cgaagcgcca ttgtgctctg taagcgagta tttacttctt caatctgctg gcgagtatgc
     5221 gccagcgccg cgctgtgttc tgcgatgcgt tcccagtgcg gacgaagatt tcgtgccggt
     5281 tgtgccagac tgagcgccgc cagttgaggt tgcgcttttt cttcttcggc taacgcctgt
     5341 tgcaaggcct gttgacggcg gctggcttct tgctgcaatt cgtccagacg cgttaaccag
     5401 tttagtgatt gttgttcttg ctgctgcgcg gtaagtaact gtttttcttc gtcagtaagt
     5461 acctgcaaac tcgctgtcag cgattgcact tgttctggcg tgagcaacgc gacgccgctg
     5521 gcctgcgctt gcagcttctc cagctctgtg cgggccgatt tgtgctgctc aaaaaccatc
     5581 gccgagattt gcccgtagat ttcagtgccg gttaactcct cgagcaattc cgcgcgttct
     5641 ttgggtttgg cattcaggaa ggcagcaaat tgcccctgcg aaagcagcat cgaacgggtg
     5701 aagcgcccgt aatccagtcc ggttaacgtc gccgtcagtt ccagcttatc tttcactttg
     5761 tcggcgagaa ttttgccatc ggcacagcgc gccagctcaa cgcgtggcac ctgcaaatta
     5821 ccgtccggct ggttacgcgc ccgattctgg ctccagaatg cacggtacgc ttcacctttg
     5881 acttcaaact ccacttccgc cagacattcg gcggtatcgc gggtcatgag atcattttgc
     5941 gattgtgaaa cgttagaaag gcgcggagtt tcgtgataca gcgccagaca aatggcgtcc
     6001 agcagggtgg ttttccccgc accggttggg ccggtaatgg caaacagccc gttgctggcg
     6061 aacggctcgc gggtgaaatc aatcttccat tcgcctttta atgagttcag gtttttcagg
     6121 cgcaggctga gaattttcat gcttcgtgtt ctccggcgag ggtatgcaac gtcgtggtga
     6181 aaagatgctg cagacgttgc tgctgcgatt catccagttc ttccagtgcc agacggcgat
     6241 tgaacacctc ttcgacgctg agttcgctga gggtttcacg ctgttggctg gctaacactc
     6301 gctcgcgctg ttcacgactc cggcgtacca gcaatacttc gacaggcaat gattcggtta
     6361 atgcctggat tttgcgctga atatcatgca gatactcatc agtagtgatt tcgatatcca
     6421 gccagacagg tggctcctgt gatacatcgc gccactgttc cagctgttcg gtaatcgacg
     6481 ccagatcgcc tttcagcacc gccatgggtt gcgttaccgg tacgttcagg ttttccacgc
     6541 tctctaattt gccgtttgaa aatgtcacca gatggacata tttactctta ccgcattcat
     6601 caaaactcag tggtatgggg gaaccgcaat agcgaacatg ttccatgccg ccaataatct
     6661 gcgcgcggtg aatatgcccg agcgcgatgt agtcggctgg tggaaagttt tgtgccggaa
     6721 acgcgtccag cgtgccaata taaatgtcac gcacggcgtc acttttactg gcacccacgg
     6781 tcgttaaatg tcccgtggcg atgatgggca gaggctgatc gccgcgcagt ttgcaggcat
     6841 cggcataatg ttgttgataa taatcagtaa tagccgccag caaatgctgc tgtttttcaa
     6901 taccgttaag ccccgcctgg ctggtaataa tgtcacgcgg acgtaaaaac ggaatagggc
     6961 acagcactgc gcctggcgtc ccgtcgcgac gaggcaagat ttgcggcgca tgtccggcgc
     7021 tggcgaccac ggtcgtattg aggaacgcca tgatatcgcg cgattcattc agcgtggcga
     7081 ccgagtcatg gtttcctgcc agtaccacca gatgacagcc agtttgctgt aaattgacaa
     7141 caaaacggtt atataacgtg cgggcgtaac tggggggcga gccggtatcg aaaacatcac
     7201 cggcaacaat aatcgcatcc acctgatggg tttgtgctgt ctccagcagc cagtcaagaa
     7261 aagcctgatg ttcagcttcg cggcttttac tgtagaagtt ctggccgaga tgccagtctg
     7321 aggtgtgaag gatgcgcata acggttccct ggcgaaaaag catgggcgcg attataccca
     7381 aacagatgtg ccatttgctt ttttctgcgc cacggaaatc aataacctga agatatgtgc
     7441 gacgagcttt tcataaatct gtcataaatc tgacgcataa tgacgtcgca ttaatgatcg
     7501 caacctattt attacaacag ggcaaatcat ggcgagacgt attctggtcg tagaagatga
     7561 agcaccaatt cgcgaaatgg tctgcttcgt gctcgaacaa aatggctttc agccggtcga
     7621 agcggaagat tatgacagtg ctgtgaatca actgaatgaa ccctggccgg atttaattct
     7681 ccttgactgg atgttacctg gcggctccgg tatccagttc atcaaacacc tcaagcgcga
     7741 gtcgatgacc cgggatattc cagtggtgat gttgaccgcc agaggggaag aagaagatcg
     7801 cgtgcgcggc cttgaaaccg gtgcggatga ctatatcacc aagccgtttt cgccgaagga
     7861 gctggtggcg cgaatcaaag cggtaatgcg ccgtatttcg ccaatggcgg tggaagaggt
     7921 gatcgagatg caggggctaa gtctcgaccc gacatcccac cgggtgatgg cgggcgaaga
     7981 gccgctggag atggggccga cagaatttaa actgctgcac ttttttatga cgcatcctga
     8041 gcgcgtgtac agccgcgagc agctgttaaa ccacgtctgg ggaaccaacg tgtatgtgga
     8101 agaccgcacg gtcgatgtcc acattcgtcg cctgcgtaaa gcactggagc ccggtgggca
     8161 tgaccgcatg gtgcagaccg tgcgcggtac aggatatcgt ttttcaaccc gcttttaacg
     8221 ccttgctcat cggacgcaga gcagggctta tgatttctta actggagtat cttacgtgct
     8281 ggaacggctg tcgtggaaaa ggctggtgct ggagctgcta ctttgctgcc tcccggcttt
     8341 catcctgggt gtattttttg gttacctgcc ctggtttttg ctggcatcgg taacaggact
     8401 gcttatctgg catttctgga atttattgcg cctttcatgg tggctgtggg tggatcgcag
     8461 tatgaccccg ccaccggggc gtggtagctg ggaaccgctg ctatacggat tacaccagat
     8521 gcagctgcga aataaaaaac gccgccgcga actgggcaat ctgattaaac gctttcgtag
     8581 cggcgcggag tcgctgcccg acgcggtggt gctgaccacg gaagagggcg gtattttctg
     8641 gtgtaacggt ctggcgcaac aaattcttgg tttgcgctgg ccggaagata acgggcagaa
     8701 catccttaac ctgctgcgtt atccggagtt tacgcaatat ctgaaaacgc gtgatttttc
     8761 tcgcccgctc aatctggtgc tcaacaccgg gcggcatctg gaaattcgcg tcatgcctta
     8821 tacccacaaa cagttgctga tggtggcgcg tgatgtgacg caaatgcatc aactggaagg
     8881 ggcgcggcgt aacttttttg ccaacgtgag ccatgagtta cgtacgccat tgaccgtgtt
     8941 acagggttac ctggagatga tggatgagca accgctggaa ggcgcggtac gtgaaaaagc
     9001 gttgcacacc atgcgcgagc agacccagcg gatggaaggg ctggtgaagc aattgctgac
     9061 gctgtcgaaa atagaagccg caccgacgca tttgctcaat gaaaaggttg atgtgccaat
     9121 gatgctgcgc gttgttgagc gcgaggctca gactctgagt cagaaaaaac agacatttac
     9181 ctttgagata gataacggcc tcaaggtgtc tggcaacgaa gatcagctac gcagtgcgat
     9241 ttcgaacctg gtctataacg ccgtgaatca tacgccggaa ggcacgcata tcaccgtacg
     9301 ctggcagcga gtgccgcacg gtgccgaatt tagcgttgaa gataacggac cgggcattgc
     9361 accagagcat attccgcgcc tgaccgagcg tttttatcgc gttgataaag cgcgttcccg
     9421 gcaaaccggc ggtagcggat tagggttagc gatcgtgaaa catgcggtga atcatcacga
     9481 aagtcgcctg aatattgaga gtacagtagg aaaaggaaca cgtttcagtt ttgttatccc
     9541 ggaacgttta attgccaaaa acagcgatta atccgccttt gtcatctttt attgccataa
     9601 gccagccgat gctggcttat tttctttgca gtcaaaatac gggcgttaga ttttacaacg
     9661 gttggcgatt ttttgttcgc atgattagcc atgtcttttt cacggaaata gtgttttata
     9721 ctagttggcg atttcttatc gctatatacc tctggttttt agatccctcc ttgctttaaa
     9781 acgttataag cgtttaaatt gcgcttcagg tgctgtcata ctgactgcat taacgcggta
     9841 aatcgaaaaa ctattcttcg ccgcgcctgg ttgggagtat ttcccgctaa aattgtttaa
     9901 atataccgct gtatcatccc cagggattgg cacaaaaatt taacgttaca acaccacatc
     9961 cacaggcagt atgatttatg acccatcaat taagatcgcg cgatatcatc gctctgggct
    10021 ttatgacatt tgcgttgttc gtcggcgcag gtaacattat tttccctcca atggtcggct
    10081 tacaggcagg cgaacacgtc tggactgcgg cattcggctt cctcattact gccgttggcc
    10141 tgccggtatt aacggtagtg gcgctggcaa aagttggcgg cggtgttgac agcctcagca
    10201 cgccaatcgg taaagtcgct ggcgtgctgc tggcaacggt ttgttacctg gcggtggggc
    10261 cgcttttcgc tacgccgcgt acagctaccg tttcttttga agtgggcatt gcgccgctga
    10321 cgggtgattc cgcactgccg ctgtttatct acagcctggt ctatttcgct atcgttattc
    10381 tggtttcgct ctatccgggc aagctgctgg ataccgtggg caacttcctt gcgccgctga
    10441 aaattatcgc gctggtcatc ctgtctgttg ccgcgattgt ctggccggcg ggttctatca
    10501 gcacggcgac tgaggcttat caaaacgctg cgttttctaa cggcttcgtc aacggctatc
    10561 tgaccatgga tacgctgggc gcgatggtgt ttggtatcgt tattgttaac gcggcgcgtt
    10621 ctcgtggcgt tactgaagcg cgtctgctga cccgttatac cgtctgggct ggtctgatgg
    10681 cgggtgttgg tcttactctg ctgtacctgg cactgttccg tctgggttca gacagcgcgt
    10741 cgctggtcga tcagtctgca aacggcgctg ctattctgca tgcttacgtt cagcacacct
    10801 ttggcggcgg cggtagcttc ctgctggcgg cgttaatctt catcgcctgc ctggtcacgg
    10861 cagttggcct gacctgtgct tgtgcagaat tctttgccca gtacgtaccg ctctcttatc
    10921 gtacgctggt gtttatcctc ggcggcttct cgatggtggt ttctaacctc ggcttaagcc
    10981 agctgatcca gatctccgta ccggtgctga ccgctattta tccgccgtgt atcgcactgg
    11041 ttgtattaag ttttacacgc tcatggtggc ataattcgtc ccgcgtgatt gctccgccga
    11101 tgtttatcag cctgcttttt ggtattctcg acgggatcaa agcatctgca ttcagcgata
    11161 tcttaccgtc ctgggcgcag cgtttaccgc tggccgaaca aggtctggcg tggttaatgc
    11221 caacagtggt gatggtggtt ctggccatta tctgggatcg cgcggcaggt cgtcaggtga
    11281 cctccagcgc tcactaaatc actgaacatt tgttttaacc acggggctgc gatgccccgt
    11341 ggttttttat tgtgttgatg ggttaggaat tgatggaaag taagaacaag ctaaagcgtg
    11401 ggctaagtac ccgccacata cgctttatgg cactgggttc agcaattggc accgggttgt
    11461 tttacggttc ggcagacgcc atcaaaatgg ccggtccgag cgtgttgttg gcctatatta
    11521 tcggtggtat cgcggcgtat atcattatgc gtgcgctggg ggaaatgtcg gtacataacc
    11581 cggcagccag ttctttttcc cgttatgcgc aggaaaacct cggcccgctg gcaggttaca
    11641 ttaccggctg gacctactgc tttgaaatcc ttattgtcgc catcgccgat gtgaccgctt
    11701 ttggtatcta tatgggtgtc tggttcccga cggtgccgca ctggatttgg gtgctgagcg
    11761 tggtgctgat catttgcgcc gtaaacctga tgagcgtgaa ggtcttcggt gagctggaat
    11821 tctggttctc gttctttaaa gtcgccacca ttatcatcat gattgttgcc ggtttcggca
    11881 tcatcatctg ggggattggc aacggcgggc aaccgaccgg tattcataac ctgtggagca
    11941 acggcggctt cttcagcaac ggttggcttg ggatggtgat gtcgctgcaa atggtgatgt
    12001 ttgcttacgg tgggatcgaa attatcggga ttaccgctgg tgaagcgaaa gatcctgaga
    12061 aatcgatacc gcgtgcgatt aactccgtgc cgatgcgtat tctggtgttc tacgtcggta
    12121 cgctgttcgt cattatgtct atctacccgt ggaatcaggt tggcactgcc ggtagcccgt
    12181 ttgtgctgac gttccagcat atgggcatta cctttgccgc cagcattctt aactttgttg
    12241 tgctgaccgc ttcgctgtcg gcaattaaca gtgatgtatt tggcgtaggc cgtatgctcc
    12301 acggtatggc agagcagggc agcgcgccga aaattttcag caaaacctcg cgtcgcggta
    12361 ttccgtgggt tacggtgctg gtgatgacta ccgcgcttct gtttgctgtg tatctgaact
    12421 acatcatgcc ggaaaacgtc ttcctggtga tcgcttcgct ggcaactttc gccacggtgt
    12481 gggtgtggat tatgatcctg ctgtcgcaaa tcgccttccg tcgccgttta ccgccagaag
    12541 aagttaaggc gctgaaattt aaagtaccgg gtggggtagc aaccaccatc ggcgggctga
    12601 ttttcctgct ctttattatc gggttgattg gttatcaccc ggatacgcgt atctcgctgt
    12661 atgtcggttt cgcgtggatt gttgtgctgt tgattggctg gatgtttaag cgtcgccacg
    12721 atcgtcagct ggctgaaaac cagtaatcct tgcgttctgt aagccggttc aggaggaaat
    12781 cctgatccgg ctattccgaa agttatccgc ccccgtcctc ctcccccaaa tatccttcag
    12841 atgatgagtg atcctgcatt aggctatggc aaggtgatca gattttcatc acaggggaat
    12901 tatgatgtta aatgcatggc acctgccggt gcccccattt gttaaacaaa gcaaagatca
    12961 actgctcatt acactgtggc tgacgggcga agacccaccg cagcgcatta tgctgcgtac
    13021 agaacacgac aacgaagaaa cgtcagtacc gatgcataag cagcgcagtc agccgcagct
    13081 tggcgtcacc gcatggcggg cggcgatcga tctctccagc gggcaacctc ggcggcgtta
    13141 tagtttcaaa ttgctgtggc acgatcgcca gcgttggttt acaccgcagg gcttcagccg
    13201 aatgccgcca gcacgactgg agcagtttgc cgtcgatgta ccggatatcg gcccacaatg
    13261 ggctgcggat cagatttttt atcagatctt ccctgatcgt ttcgcccgta gtcttcctcg
    13321 tgaagctgaa caggatcatg tttattacca tcacgcagcc ggacaagaga tcatattgcg
    13381 tgactgggat gaaccggtca cggcgcaggc gggcggatca acgttctatg gcggcgatct
    13441 ggacggtatc agcgaaaaac tgccgtatct gaaaaaactt ggcgtgacgg cgctgtatct
    13501 taatccggtg tttaaagctc ccagcgtaca taaatacgat accgaggatt atcgccatgt
    13561 cgatccgcag tttggcggtg atggggcgtt gctgcgctta cgccacaata cgcagcagct
    13621 gggaatgcgg ctggtgctgg atggcgtgtt taaccacagt ggcgattccc atgcctggtt
    13681 tgacaggcac aatcgtggca cggggggagc ttgtcacaac cccgaatcgc cctggcgcga
    13741 ctggtactcg tttagtgatg atggcacggc gctcgactgg cttggctatg ccagcttgcc
    13801 gaagctggat tatcagtcgg aaagtctggt gaatgaaatt tatcgcgggg aagacagtat
    13861 tgtccgccac tggctgaaag cgccgtggag tatggacggc tggcggctgg atgtggtgca
    13921 tatgctgggg gaggcgggtg gggcgcgcaa taatatgcag cacgttgctg ggatcaccga
    13981 agcggcgaaa gaaacacagc cggaagcgta tattgtcggc gaacattttg gcgatgcacg
    14041 gcaatggtta caggccgatg tggaagatgc cgccatgaac tatcgcggct ttaccttccc
    14101 gttgtgggga tttctcgcca ataccgatat ctcttacgat ccgcagcaca ttgacgccca
    14161 aacctgtatg gcctggatgg ataattaccg cgcagggctt tctcatcaac aacaattacg
    14221 tatgtttaat cagctcgata gccacgatac tgcgcgattt aaaacgctgc tcggtcggga
    14281 tattgcgcgc ctgccgctgg cggtggtctg gctgttcacc tggcctggtg taccgtgcat
    14341 ttattacggt gatgaagtgg gactggatgg taaaaacgat ccgttttgcc gtaaaccgtt
    14401 cccctggcag gtggaaaagc aggatacggc gttattcgcg ctgtaccagc gaatgattgc
    14461 gctgcgtaag aaaagtcagg cgctgcgtcg tggcggctgt caggtgctgt atgcggaaga
    14521 taacgtggtg gtatttgtcc gcgtgctgaa tcagcaacgt gtactggtgg caatcaaccg
    14581 tggcgaggcc tgtgaagtgg tgctccccgc gtcaccgttt cttaatgtcg cgcaatggca
    14641 acgcaaagaa ggccatgggc aactgacaga cgggattctg gctttgcctg ccatttcggc
    14701 tacggtgtgg atcaactaaa gcatctgttt tatttgttac gtcgttagtc accagccctg
    14761 acactacgca gcgcaaaaaa agctgctcaa tgtcagggct gagtttgtta aatatttgtc
    14821 ccgttcgata tgagcgattt ttggtattct tttcccatga ggttatgtac aacctgctcc
    14881 aggataaccc tgtattggag caaagatatg acaatttcaa attcgaattt attgaaaccg
    14941 tttattactg gtactgagtt gccgcctttt aaagaggatg tcttttctaa aacagaagaa
    15001 caatttaaaa aaagccccca tggcaaattg cttttcattt ccggaaatga aaacttagta
    15061 aacatttcag cggaaaaaat cgatgaaata aaaaatagca aagatttctt tt
//
LOCUS       DAYPHN010000118        15072 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000118 DAYPHN010000000
VERSION     DAYPHN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.118"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(107..2386)
                     /gene="maeB"
                     /locus_tag="ACG0BW_003403"
     CDS             complement(107..2386)
                     /gene="maeB"
                     /locus_tag="ACG0BW_003403"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFK7260328.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2675..3625
                     /gene="tal"
                     /locus_tag="ACG0BW_003404"
     CDS             2675..3625
                     /gene="tal"
                     /locus_tag="ACG0BW_003404"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFK7260329.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3645..5648
                     /gene="tkt"
                     /locus_tag="ACG0BW_003405"
     CDS             3645..5648
                     /gene="tkt"
                     /locus_tag="ACG0BW_003405"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFK7260330.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKKAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5751..6794)
                     /gene="ypfG"
                     /locus_tag="ACG0BW_003406"
     CDS             complement(5751..6794)
                     /gene="ypfG"
                     /locus_tag="ACG0BW_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFK7260331.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATLTAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6920..7495)
                     /gene="nudK"
                     /locus_tag="ACG0BW_003407"
     CDS             complement(6920..7495)
                     /gene="nudK"
                     /locus_tag="ACG0BW_003407"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFK7260332.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7563..9542)
                     /gene="aegA"
                     /locus_tag="ACG0BW_003408"
     CDS             complement(7563..9542)
                     /gene="aegA"
                     /locus_tag="ACG0BW_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFK7260333.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9748..11448
                     /gene="narQ"
                     /locus_tag="ACG0BW_003409"
     CDS             9748..11448
                     /gene="narQ"
                     /locus_tag="ACG0BW_003409"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFK7260334.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNLELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11612..14725
                     /gene="acrD"
                     /locus_tag="ACG0BW_003410"
     CDS             11612..14725
                     /gene="acrD"
                     /locus_tag="ACG0BW_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFK7260335.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHEALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14824..14883)
                     /gene="ypfM"
                     /locus_tag="ACG0BW_003411"
     CDS             complement(14824..14883)
                     /gene="ypfM"
                     /locus_tag="ACG0BW_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFK7260336.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3526 a         4011 c         3935 g         3600 t
ORIGIN      
        1 actgcttttt ttattattcg taaactcagt aataagcttt actaatatat ttcaggaaaa
       61 ccgcttaccc tgaatattca gggtaagcgt gagagttaag aatgaattac agcggttggg
      121 tttgcgcttc aaccacagcc agcgccacca tgttgacgat acgacgtacc gatgcgatcg
      181 gcgttaacac gtgaaccggt ttcgccacac ccatcagcac cgggccgaca gtcacacctt
      241 ccgagctgga aacgcgcaat aagttgtaac taatgcgggc agcttccatg ttcggcatca
      301 ccagaatatt agcggaacct ttcaaagggc tgtccggcat acggtcattg cgaatcgctt
      361 ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagttctggt gcacgttccc
      421 tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt
      481 tggagtgcga caacagagcc acacgcggct caataccaaa acgacgaaca gtttctgccg
      541 ccatcaaggt gatctccgcc agctcttctg cgtctggttc gtcattgacg tatgtatcgg
      601 caataaaggt gttaccactc ggcagcagca gcgcgttcat ggcacctgcg gtgtgaacgc
      661 catcgcgata accaaagaca ttttttacca cgctaaaatg ttcatgataa tcgcccaccg
      721 taccgcagat cattgcatcg gcttcgccac gctgaaccat gattgcgccg atcactgtcg
      781 ggttactgat caatgcacgc tgcgcctgtt cctgagtgac gccgcgacgc ttcatgatct
      841 ggaagtattc agtccagtac tctttaaagc gcggatcgga ttcgttattg acgatctcaa
      901 aatcaacacc cgctttgatc tgcaagccca gtttctgaat gcgcatttcg atcacgttcg
      961 gacgaccgat aaggatcggt ttcgccagcc ccagcgttac cagttcctga gtggcatgca
     1021 gaacgcgcgc ctcttcccct tccggcagaa cgacgcgctt cggcgctttg cgagcctggg
     1081 agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctccgtcagc ttgtcgatgt
     1141 agacgtcgaa atcagcaatc ggacgcgtcg ccacgccgga ctccatcgcc gctttcgcca
     1201 ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg atgtattccg
     1261 gaccaaagct cagatcctga tcgccataag ctgaagccac cacttcactc tgttccgcat
     1321 gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg atggcggttg
     1381 cgccaacgtc cagcgcgcca cggaagatga acgggaagca aagaacgttg ttcacctggt
     1441 tcgggtagtc ggaacggccg gtacagatga tggcatccgg acgcacttct ttcgccagcg
     1501 gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggcgca cgggccattt
     1561 tcttcaccat ttcctgggtc agcactttcg ggccggaaca gcccaggaaa atatccgcgc
     1621 cttcaatcac atcatcgagg gtacgtttgc cgtcatccac caccgcatat gcggctttgg
     1681 tttccgccat gtttggctca cggccctgat agataacgcc ttttgagtcg caaaccacga
     1741 tgttatgttt ttgcagaccc agcgctacca gcaggttcat acaggcgatt gctgcggcac
     1801 ccgcaccgga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg cgcaaaccgt
     1861 tgaggatggc ggcagtgctg atgattgccg tgccgtgctg atcgtcgtgg aataccggaa
     1921 tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt
     1981 cgaggttgat gccgccgaag gtcggttcaa gcgcggcgac gacttcaata aatttgtccg
     2041 ggtcgagttc atcaacttca atgtcaaata catcaattcc ggcgaatttc ttaaacagaa
     2101 cgcccttgcc ttccatcacc ggtttgcccg ccagcgcgcc aatgttgcct aaccccagca
     2161 ccgccgtacc gttagagatc accgccacca ggttaccgcg cgcggtatat ttgtaggctt
     2221 ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag taggccagcg
     2281 ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc cctggaactg
     2341 gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg ttgttccttt
     2401 cacgtaacgt tcacaaataa agtgtgtggg caacagcccc tgcccacaac gtggcgcaca
     2461 ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc attaatataa
     2521 tgttttggta ataatcctat aacactgatg ttacctgctt aatccagcaa taccatgcct
     2581 gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact
     2641 catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg gcatcaaaca
     2701 gttcaccact gtcgtggcag acagcggcga tattgagtcc attcgccatt atcatcccca
     2761 ggatgccacc accaatcctt cgctgttact caaggctgcc ggattatcac aatatgagca
     2821 tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg aacaacaggt
     2881 ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaattctca aaatcgtacc
     2941 cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa agagtattga
     3001 gaaggcgcgc catctggtgg acttgtatca gcaacaaggc gttgagaaat cacgcattct
     3061 gatcaagctg gcttcgacct gggaaggaat tcgcgcggca gaagagctgg aaaaagaagg
     3121 tattaactgc aacctgacgc tgctgttttc ttttgcccag gcgcgggcct gtgcagaagc
     3181 aggcgttttt ctgatttcgc cgtttgtcgg gcgtatttat gactggtatc aggcacgcaa
     3241 gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt aaatcggtgc gcaatatcta
     3301 cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct tccgtcgcac
     3361 cgaacaaatc ctcgccttaa ccggctgcga tcgactgact atcgcaccga atttgctgaa
     3421 ggagctgcag gaaaaagttt cgccagtggt acgtaaatta atcccacctt ctcagacttt
     3481 cccacgccca gctcccatga gcgaagcgga gttccgttgg gagcacaatc aggatgcgat
     3541 ggcggtagaa aaactgtctg aaggcattcg tctgttcgcc gttgatcaac gcaaactgga
     3601 agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc cgaaaagacc
     3661 ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca aaaagccaat tctggtcatc
     3721 ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat tttcttaaac
     3781 ataaccccac cgacccaacc tggtatgatc gcgaccgctt tattctttcc aacggccacg
     3841 cgtcgatgct gctctacagt ttgctacatc tgaccggtta cgacctgccg ctggaagaac
     3901 tgaagaactt ccgtcagttg cattcaaaaa ccccaggtca cccggagatt ggctatacgc
     3961 caggtgttga aaccaccacc ggtccacttg gacaaggttt ggcgaacgcc gtcggactgg
     4021 cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc ggatcatgaa attgtcgatc
     4081 acttcaccta tgtgtttatg ggcgacggtt gcctgatgga aggtatttcc cacgaagtct
     4141 gttcgctggc gggcacgctg ggactgggca agctgattgg tttttacgat cacaacggta
     4201 tttccatcga cggtgaaacc gaaggctggt ttaccgacga tacagcaaaa cgttttgaag
     4261 cctatcactg gcatgtgatc catgaaatcg acggtcacga tccgcaggcg gtgaagaaag
     4321 cgatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc cgcacagtaa
     4381 ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca ccgctggggg
     4441 aagaagaagt ggcgctggca cggcaaaaac tgggctggca ccatccgcca tttgagatcc
     4501 ctaaagagat ttatcacgcc tgggatgccc gcgaaaaagg cgaaaaagcg cagcagagct
     4561 ggaatgagaa gtttgccgcc tataaaaagg cccatccgca actggcagaa gagtttaccc
     4621 gtcggatgag cggtggttta ccgaaggact gggagaaaac gactcagaaa tatatcaatg
     4681 aattacaggc gaatccggcg aaaatcgcca cccgtaaggc ttcgcaaaat acgcttaacg
     4741 cttatggccc cttgctaccg gagctgctcg gcggttcggc ggatctggct cccagcaacc
     4801 tgaccatctg gaaaggttct gtgtcgctga aggaagatcc ggcgggcaac tacattcact
     4861 acggggtgcg tgaatttggc atgaccgcta tcgccaacgg cattgcgcac cacggcggct
     4921 ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac gccgcgcgga
     4981 tggcggcact aatgaaagcg cggcagatta tggtttatac ccacgactca attggtctgg
     5041 gcgaagatgg tcctacgcac caggctgttg agcaactggc cagtctgcgc ttaacgccaa
     5101 atttcagcac ctggcgaccg tgcgatcagg tggaagcagc ggtgggctgg aagctggccg
     5161 ttgagcgcca caacggaccg acggcactga tcctctccag gcagaatctg gcccaggtgg
     5221 aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg ttatgtactg aaagacagcg
     5281 gcggtaagcc agatattatt ttgattgcca ccggttcaga gatggaaatc accctgcaag
     5341 cggcagagaa attagcggga gaaggtcgca atgtacgcgt agtttccctg ccctcgaccg
     5401 atattttcga cgcccaggat gaggaatatc gggagtcggt gttgccttct aacgttgcgg
     5461 ctcgcgtggc ggtggaagca ggcatcgccg attactggta caaatatgtt ggtctgaaag
     5521 gggcaattgt cgggatgacc ggctatgggg aatctgctcc ggcagataag ctgttcccgt
     5581 tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtgctg ggagtgaaag
     5641 gtgcctgatg gtgatcgccg gatgctgtat gttcccctca ccctaaccct ctccccagag
     5701 gggcgagggg acggtccgtg cgctttttag gggcgcatct gggattttgt ttaacgggtg
     5761 atccacaacg tgggccaggc gtctggccca tgccagttat cacaggtggg ttctgcggcg
     5821 taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg
     5881 cttaatccgc gcccttttgc taaggtcacc agttcacgcg atttttcatc gaatgttgcg
     5941 ttcatcagtt ccagttcatt cgtctcctga ccgctgttga acggcaaacg taaccgaacc
     6001 gggcgcgagg ccagtggctt tttacgcgac acaatccagg ccaaatcaat ggtgttatag
     6061 gcccctgcct cacagctaat catcatcagc gctttatcat cggtcagcgc agtgacattt
     6121 acctctcgac gcaatggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa
     6181 tccagcaaat cgttgcgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg
     6241 acctctttca gcgcaggcgc gggcggtacg ctgagcggcg gttcatcccc tttctttatc
     6301 cacgcggttt cactgccaac gcgcttttgc tgagcatcta taaacaacaa cgccgctttt
     6361 aagccactca gagaaatggt ctgattgcca tcgcgtaagg tgattgcctt cccttcctga
     6421 atcatctgca aaaacgcggt gagggttgcc gtatcatcgg ttactaataa ccatggtgaa
     6481 atccgccact tgtcaccact taatgccaac ggctcaccat ctaacagcag ccgtggcgct
     6541 atctcccctt ccgacgcatc cggcgacttc aacccgccgc gctcaatacg taaaacggca
     6601 tcggtatgcg ccccagcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta
     6661 ttgcgcgcca cacaaaaatt ttgattatta caggtgacct gccagtcgga aaacgcccgt
     6721 tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa
     6781 atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc
     6841 ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat aataaacaac
     6901 gaaatattat tgttattttt cagtccatta aatgtgacgt ttgcaaatag ttaagcaata
     6961 acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg
     7021 gcagctcgag cacttcaata tcttcatctt cgacaccgcc accggcgtta gcgcgctgat
     7081 tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcct ggggacatat
     7141 acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcctctt
     7201 tgcgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagtt
     7261 gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgg
     7321 tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac
     7381 gtttatgacg gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg
     7441 tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga
     7501 ctccacagcg cgaaatgaac aattatctta cgctgtgaag ccgggtttgt ctgcgcaggc
     7561 tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga
     7621 cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc accaccggcg
     7681 aagattttcg gattcgtggt ctgataacgg tactggcttt ccacatccgc gatgatgcgg
     7741 ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg tggattgaag
     7801 ccaaacgcca taatcaccgc gtcggctggc atgacaaatt cactgccttc caccggcact
     7861 ggacgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaattccg
     7921 caaacgtgac cttgctcatt cagctcaagc gccaccggct ggacgttaaa ttcaaagttg
     7981 gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca
     8041 cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcggtacg cacacagtcc
     8101 atcgcggtgt cgccgccgcc cagtaccacg acgttgagtc cggcggtatt gataaacggc
     8161 tcttccggta gctcttcgag tcccatcacc tgtttagtgt tggcgatgag gaacggcagg
     8221 gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg
     8281 cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta
     8341 cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt
     8401 gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccaatt
     8461 tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atccgcacag
     8521 gccagccctg ccggacctgc accgataatc gccacccgct tgtccacttt ggtgacatgg
     8581 cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatgtagcg ttcaatgttg
     8641 ccgatagtta ccgcaccgtg ctcatcgcga atagtacagg ctccttcaca caaacggtct
     8701 tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg
     8761 gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggaatatg gttatgcagc
     8821 gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt
     8881 tgtgcctgat cggcacggaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca
     8941 atcgccagtt tgtccggctc gccgcgcgcg ggcgttgcct gcatttgctc gactttactc
     9001 attaccggca tttcttgtgc cgcggtactg gcatgccacg gttgatgctc ctgacgtgcg
     9061 gtgcgcaagc ggcgggattt cgccatgccg gagagtgcgg cgtcagtgac cagttgcagc
     9121 gcgtctgccg ggcaattctc aacacaggca ggaccgtttt cgcgccctgc acaaaggtcg
     9181 catttatgcg ccgtggcttt tactttgccc gccgcgacgg gtgtcaggac gatttgcatc
     9241 gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc
     9301 tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca
     9361 tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat aaccgtaatt
     9421 cggggatgaa aatggtgttg gctcaagaca tgttgctcat cattgtgagc catgacacag
     9481 gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc
     9541 ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc
     9601 cgacttacag gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa
     9661 actgtggcag atcaaataat cccatcactg actaaattgc gtttcagcga actggaacat
     9721 taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg
     9781 gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta
     9841 actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg
     9901 cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc
     9961 cagttatttc agcaggcact gcattcaccg gtattaacca atctcaacgt ctggtatgtg
    10021 ccagaagcag taaaaacccg ctatgcgcat ctgaatgcca actggctgga gatgaataat
    10081 cggctcagca agggcgattt accgtggtat caggccaata ttaataatta tgttaatcag
    10141 atagacctgt tcgtactggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg
    10201 gcgatttccc tggctggcgg catcggtatt ttcacgctgg tcttttttac tttgcgccgc
    10261 atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa
    10321 cacgggcagt tcgactcgcc gccgctggat accagcctgc cgaatgagct tggcctgctt
    10381 gccaaaacct ttaaccagat gtcgagcgag ctgcataaat tgtaccgttc gctggaggcg
    10441 tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgcctgga ggtgttgtat
    10501 cagtgttcgc aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg
    10561 cagattgtgc gcgacaacga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg
    10621 cggattagcg aagggcaacc aaacctggaa ttgccgatgc agattttacc ggtgacaatg
    10681 caagagacgg tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg
    10741 ctgcttaaca gtgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag
    10801 cattttcagc agttattgtt gatggaagaa cgtgcgacca tcgcccgcga attgcacgac
    10861 tcgctggctc aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata
    10921 ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat
    10981 gcttatcggc agttacgcga gctgttaact actttccgcc tgacgctgca gcaggcagat
    11041 ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag cgctaaactg
    11101 accctcgact gccgcctgcc aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg
    11161 caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc
    11221 gttagctgcg tcaccgcgcc ggacggcaat cacacagtct atattcgcga caacgggatt
    11281 ggcatcggtg aaccgaaaga acccgaaggc cattatggtc tgaatatcat gcgcgaacgc
    11341 gcggaaagat tgggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg
    11401 agtattagct ttcgctccgc ggagggtgag gaaagtcagc tgatgtaatg cctccgactg
    11461 accaaagaat actggccctt aaagttcagt ataaaagggc atgataattt acattaactc
    11521 ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca
    11581 acgatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat
    11641 ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt accctggcga ttttttcatt
    11701 gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc
    11761 cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac
    11821 cggcctcgat aatctgatgt atatgtcgtc tcagagcagt ggcaccggcc aggcatctgt
    11881 cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca
    11941 gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg
    12001 taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg gttcgatgga
    12061 taaacaggat attgccgatt acgttgccag taatattcag gatccgttaa gccgcgtgaa
    12121 tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc
    12181 ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca ttgagtcaca
    12241 gaacgcgcag attgcggttg ggcaacttgg tggtacgcct tccgtcgata agcaggcgct
    12301 caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac
    12361 cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat
    12421 gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggttggg
    12481 ggtaaaactg gcctccggcg cgaacgaaat ggcgacagcg gagctggtgc tcaatcgtct
    12541 tgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt atgaaaccac
    12601 ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct
    12661 ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat
    12721 cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tatgccttcg gttacagcgt
    12781 caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgacgccat
    12841 cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc
    12901 cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc
    12961 ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt
    13021 ctctattacc attgttgcgg cgatggtgct gtcagtattg gtagcgatga tcctcactcc
    13081 ggctctgtgt gccacgctac ttaagccact gaaaaagggt gagcatcacg ggcaaaaagg
    13141 cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt
    13201 ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg
    13261 catggtgttc ctgttcctgc gtttgccgac gtcgttcttg ccactggaag accggggcat
    13321 gtttactacc tcggtacagt tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt
    13381 tgagcaaatc gagaaatact acttcaccca tgaaaaagac aacatcatgt cggtgtttgc
    13441 cactgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt ttatccgcct
    13501 gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc
    13561 aacgaaggcg tttaacaaga ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat
    13621 tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg ctggagcggg
    13681 tcacgaggcg ctgatggcag cacgtaacca gttgctggcg ctggcagcgg aaaacccgga
    13741 gctaacccgt gtgcgccata acggcctcga cgacagtccg cagttgcaga ttgatatcga
    13801 ccagcgtaaa gctcaggcgc tgggcgttgc tatcgacgat attaacgaca cactgcaaac
    13861 cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta
    13921 tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg
    13981 aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg
    14041 ctcgccgcgt ctggaacgct ataacggtta ttctgcagtt gagattgttg gggaagccgc
    14101 accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga agcagctgcc
    14161 aaacggcttt ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca
    14221 ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg cggcgctgta
    14281 tgaaagctgg tcggtgccgt tctcggtaat gctggtcgtg ccgctggggg ttatcggcgc
    14341 gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt
    14401 aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat
    14461 gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg
    14521 cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac
    14581 gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc
    14641 ggccactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt
    14701 cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat gtgtcgccct
    14761 tttcaacttt ccgattaaga acctgctcag caggttcttg ctgtttgtac tttgtctcag
    14821 gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat
    14881 cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct
    14941 aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg
    15001 tcctgcggtg atgatttgta tgtgatacac agcaacattt cgagatattc atacggcatc
    15061 taatactgat tt
//
LOCUS       DAYPHN010000119        15021 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000119 DAYPHN010000000
VERSION     DAYPHN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.119"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..637)
                     /gene="ybtQ"
                     /locus_tag="ACG0BW_003412"
     CDS             complement(<1..637)
                     /gene="ybtQ"
                     /locus_tag="ACG0BW_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFK7260337.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVE"
     gene            complement(624..2336)
                     /gene="ybtP"
                     /locus_tag="ACG0BW_003413"
     CDS             complement(624..2336)
                     /gene="ybtP"
                     /locus_tag="ACG0BW_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFK7260338.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            2593..3552
                     /gene="ybtA"
                     /locus_tag="ACG0BW_003414"
     CDS             2593..3552
                     /gene="ybtA"
                     /locus_tag="ACG0BW_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFK7260339.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRHHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNNIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            3744..9851
                     /gene="irp2"
                     /locus_tag="ACG0BW_003415"
     CDS             3744..9851
                     /gene="irp2"
                     /locus_tag="ACG0BW_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFK7260340.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLCEVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKSFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            9939..>15021
                     /gene="irp1"
                     /locus_tag="ACG0BW_003416"
     CDS             9939..>15021
                     /gene="irp1"
                     /locus_tag="ACG0BW_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFK7260341.1"
                     /translation="MDNLRFSSAPTADSIDASIVQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYHHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPNAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPG"
BASE COUNT         3067 a         4670 c         4263 g         3021 t
ORIGIN      
        1 attcaacgat atcgccgctc agacgctggt gcgcttcccc cagcgtctgc atttgtcgcc
       61 gcatcgccgg gcggcgccag taataaaacg gcaccagcag agggaagatc agcaacatca
      121 ccagccccag ccgccagtct atccacaatg tcgccagcga cgccgtcagc ggcgtgacaa
      181 tggtgagcag caaaatattg gctatcgcaa taacataatt gaggttttca tccacgctgc
      241 ccagcagcaa ggcgttcatt tcacccgccc tgccgcgctg gagcttctcc agcggcacgc
      301 ggcgtaactg ctcgccaagt cgcaggcgca actcatgggt agcctgcgcc agatgaccac
      361 ggtattcaaa gcccaggcca taccagcgta gcaccagcgt cacaattgcg gcgacgctga
      421 aggccatagc ccagttaaga agttgcggcg cgtctccccg taacagcgca tcaatgatcg
      481 gataaagaca ggcaaaggcg atcccctgca tgaatgccgc cagcaacagc gccagcatac
      541 tgcgtcgcag catcctggcc tgactgccaa cgctggagat aagctggcgc cagatgacgc
      601 gccaggccag gttatccgca ggattattgt ctttcatttt ccaccacctc ttcctgtgtt
      661 ccacccggca cccaatgctg cgcctgctga tagtgttgcc agagccgttg atacaggccg
      721 ccctgcgcca acagttgcgt atggttcccc atttccctga gctgtccgtc ggaaaacagc
      781 aatatcacat cggcctgagt caccattgag agacgatgcg cgaccatgat gaccgtccgg
      841 ccacgcatgg ccgccgcgag cgccttgata agcgccgctt cgttttccgg gtcagcaaac
      901 gccgtcgcct catcgagcac caggatgggg cgatcctgca aaagcgcccg ggcgatagta
      961 atgcgctgcc gctggccgcc ggagagaaat accccacgtt ccccgactcg agtgttgtaa
     1021 ccttctggca gagcgctaat aaaatcatgg gcctgcgcca ctctggccgc cgctataacc
     1081 gcctccagcg gcgtatccgg cgcgcccaga cgaatgttat tggctatcgt gtcggcaaaa
     1141 aggaagttgt cctgaaacac aaacgagagt tgcttcatca gggtgtccgt ctgcatatca
     1201 cgcagatcca cgccgccaat acggatatgg cctttgtccg ggtcggcgta acgcagcagc
     1261 aagcgcgcca cggtgctttt tccggcgccg cttggcccga ccagcgccac aatttgcccg
     1321 gcaggcacat gaaagctcac ctcctgcaac gcggcgccag tacgcgcttg cggataatga
     1381 aagctcacct gctcaaaggt aatgctcgcc tcttgcggct gctggtcaga ctgcggcagc
     1441 gataactccg gcatcgcgag aacctgataa atacgctgaa tgcttaaacg cgtttgcgcg
     1501 accaggttat tgagcatcat cattggcatt acggcttcgg ccattccgct gcccagcagt
     1561 aaaacggcca cccacgcgat aaaatcgaaa ctgccatagt gcaacaggcc gtatcccgac
     1621 cagatcagga caaacagggt cgggagagga ttcagaatcg agaaggaaaa acgcgctgaa
     1681 aaaccggctt tgcgatacca ggttttgagc acatcgaccc actcttcaag ggcgcgttga
     1741 tagcgcaaaa aactggtgct gccgctatca aacgttctca ccacgggcat ggcctgcacg
     1801 aactcaataa ccgccgcgct aacctgctcg cgcgccttat gataacgctg agccatattt
     1861 tccgaacgct ggcgggcgag cacgagaaca accgatccaa acgccagtac ccccagcgtc
     1921 gcgattgcca gccgccagtc cagccagaac aagataacga ttgtcgccag cggcatgatg
     1981 atcgcgcggg catagagcgg cgtgctgtcg gccacaaaaa tatgtaacga cttcacgtca
     2041 tccagcatca ctttcgccac tgacccgctg cccatttgct gtaacacgcc aggcggaagc
     2101 tgcaatgctt tacgggctaa cctgctgcgc agaatgttct ccagatgaaa agccgcgtaa
     2161 tgagaggtgt tgaacgcctg taagcgcaga acaaaagtta agaccacgca gccgatcacg
     2221 ccgcccagcg gccaggccgg aatagcgtca ggcgtcgcgc gaatgtcacg caggctccac
     2281 gccagaaaaa ggagcgcccc caggctggtt aatgccgcca gccccgccag cgccatagct
     2341 aaaataacct tcctgcgaac cggtgtaatc acctgccaga gaggaaatcg ggacagcgat
     2401 tcagtattgg atgattgaga tgacatggga gtaactgaat ttcctgatga atttttacgg
     2461 acgccaaaaa cgacaattca gggtggcgtt ctgagaatta atgtgaataa taaccattat
     2521 caataactca tctaccccat tcggttctat ttatacccgc attggtctaa gccacaggga
     2581 gataaccagg tcatgacgga gtcaccgcaa acgcaatctg aaatctctat tcaccagttg
     2641 gtggtcggga aacctgcaaa cgatggcaat attcctgctc agtgtgaact gctgcgctgt
     2701 tcgttgcagg aaggtatgga tattttgctc tggaggggcc attttgcgcg gccagagacg
     2761 ttgcagctac acgacgatct gggcaggatt aacttctcgt gcatcctgga aggcacatcg
     2821 cgctttgcga ttcagggact ccggcaccac accgactggg agcttgcgcg caatcgccat
     2881 tacattaccc atacgccaga ctgtcgcggc agcgcctcct attgtggccg attcgagagc
     2941 attaccctct ccttcagccc tgaaaccctc gcgctgtggg tgccggatat tagcgcggtc
     3001 attaagaata atatcgactc ccactgctgt tgccagcagc agcgttgtaa tgctgaaacc
     3061 cacttaacgg ctcaggcctt gcgtcacgcg ctgatgcgca tgcacggcgg cttcagtcat
     3121 gaacaaaagc cgtcgacgct atggttgcag gggcaaagtc tggtcatgct cagcctggtg
     3181 ctggatgaac atcgcgaaga cgccagttgc ctctcctgcc acttcaaccc gatggaacgc
     3241 cagaaactgc tgcgggcgaa ggatctgctg ctggctgatc tgacgcaagc gccgggcgtc
     3301 gcggagctgg ccagagagtc cggtctgagc gttctcaaaa ttaagcgagg cttccgcgtt
     3361 ctgttcaaca acagcgtcta tggcctgttt caggctgaac gaatgcagga agccagaaga
     3421 cgcctggcca acggcaatac ctccgtcatg acggtggcgg ccgatctggg ctatgccaac
     3481 gccagccatt tctctgccgc cttccagaaa cagtttggcg taactccttc gacctttaaa
     3541 cgcgggatgt aaccggggtc gcgccccccc taaactcctc cctgacagag gcccggatac
     3601 cccatccggg cctctgttct ttccgtctgt taccaacccg aaacgggcat aaataaaccc
     3661 gtttcgggta gcatccaatt gttaataatt attattctca tatgagtaat gcttttcggt
     3721 aagacgtgcc atcaggagga agaatgattt ctggcgcacc atctcaggat tcgctgttac
     3781 cggacaaccg ccacgcggct gattaccaac aattacgcga gcggctcata caggaactga
     3841 atttaacgcc gcagcagtta catgaagaga gcaacctgat ccaggccggc ctggattcca
     3901 taagattgat gagatggtta cactggtttc gtaaaaatgg ctaccgcctt acccttcgcg
     3961 agctgtatgc cgcccccacg ctggcggcat ggaaccagtt aatgctcagc cggtcgccgg
     4021 agaacgcgga agaagaaacg ccgcccgacg aatcatcctg gccgaacatg accgaaagta
     4081 cccccttccc attgacgcca gtacagcacg cctacctgac gggccgcatg ccggggcaga
     4141 cgcttggcgg cgtgggttgc cacctgtatc aggagtttga aggccattgt ctgacggcgt
     4201 cgcagctgga gcaggccatc acgaccttgc tgcaacgcca cccaatgctg catatcgcct
     4261 ttcgccccga cgggcagcag gtctggctac cgcaacctta ctggaacggc gtcaccgttc
     4321 atgatttacg ccataacgac gctgaaagcc gccaggccta tctggacgca ctgcgccagc
     4381 gcctgagcca ccgtctttta cgcgtggaaa tcggcgaaac gtttgatttt cagctgacgc
     4441 tcttgccgga caatcgccac cgcctccatg tcaatattga cctgctgatt atggatgcct
     4501 ccagctttac gcttttcttc gatgagctta acgccctgct ggccggagaa tcgctgccgg
     4561 ctatcgacac ccgctatgat ttccgctcgt atttgctgca ccagcagaag atcaatcaac
     4621 cactgagaga cgacgcgcgc gcttactggc tggcgaaagc atcgacgctt ccccccgcgc
     4681 ccgtcttgcc gctggcctgc gaacccgcca cgctatgtga agtccgtaat acccgacgcc
     4741 gcatgattgt cccggcaaca cgctggcacg cctttagcaa ccgggccggc gagtatggcg
     4801 tgacgccgac aatggcactg gcgacctgtt tttctgccgt gctggctcgc tggggcggcc
     4861 tgacgcgtct gctgcttaac atcaccttat tcgaccgcca gccgctgcac ccggcggttg
     4921 gcgcgatgct tgccgacttc accaatattc ttctgctgga taccgcctgc gatggcgata
     4981 ccgtcagcaa cctggcgcgt aaaaaccagc tcacgtttac ggaggactgg gagcatcgcc
     5041 actggtccgg cgtcgaatta ctccgtgaac tcaaacgcca gcagcgctac ccccacggcg
     5101 ccccggtggt atttaccagc aatctggggc gttccctcta cagcagccgc gcagaatcgc
     5161 cgttgggcga gccggaatgg ggcatctcgc aaacgccgca ggtctggata gatcatctgg
     5221 cgttcgagca tcacggcgag gtctggctac aatgggacag caacgacgcg ctgttccctc
     5281 cggcgttagt cgaaacattg ttcgacgcct actgccagtt gattaaccaa ctctgcgatg
     5341 acgaaagcgc ctggcaaaag tcgttcgcag atatgatgcc cgccagccag cgcgcgatac
     5401 gcgaacgggt caacgccacc ggcgccccca ttcccgaagg cttgctgcat gaaggcattt
     5461 tccgtatcgc tctgcaacag ccgcaggcgc tggcggtaac ggacatgcgt tatcagtgga
     5521 attatcatga gctgacagac tatgcccgcc gttgcgcggg caggttaatc gagtgcgggg
     5581 ttcagcccgg cgataatgtg gctatcacga tgtcgaaagg cgcaggacaa cttgttgcgg
     5641 ttctggccgt cctgctggcc ggggcggttt acgttccggt ttcgctggat cagcctgccg
     5701 cacggcgcga gaaaatctac gctgacgcca gcgtccggct ggtgctcatt tgtcagcacg
     5761 acgccagcgc cgggtcagac gatattcccg tccttgcctg gcagcaggcc attgaggcgg
     5821 agccgatcgc caacccggta gtacgcgccc ccacgcaacc ggcctacatt atctacacct
     5881 ccggctctac cggtacgccg aaaggggtag tcatttctca ccggggagcg cttaacacct
     5941 gttgcgatat caatacccgc tatcaggttg gcccgcatga cagggtgctg gccctctccg
     6001 ccctacattt tgatttatcg gtttacgaca tttttggcgt actgcgcgcg ggcggcgcgc
     6061 tggtgatggt gatggaaaat caacggcgcg atcctcacgc atggtgtgag ctgatccagc
     6121 gccatcaggt cacgctctgg aacagcgtcc cggcgctgtt cgatatgctg ctgacctggt
     6181 gtgaaggttt cgccgacgcc acgccggaaa acctgcgcgc agtgatgctt tccggcgact
     6241 ggatcgggct tgacctcccc gcccgttatc gggccttccg gccacaagga caatttatcg
     6301 cgatgggcgg tgccaccgag gcgtctatct ggtctaacgc ctgcgaaatt cacgacgtcc
     6361 ccgcccactg gcgctccatc ccttacggtt ttccgctaac caaccaacgc taccgggtgg
     6421 tggatgaaca gggccgggac tgccctgact gggtgccggg tgaattatgg attggcggca
     6481 ttggggtcgc ggaaggctat ttcaacgatc ccctgcgtag cgagcagcaa tttttgacgc
     6541 tcccggacga gcgctggtat cgcaccggcg atctcggctg ctactggcca gatggcacaa
     6601 tcgagttcct cggtcgtcgc gacaagcagg tcaaagtcgg aggatatcgc atcgagctgg
     6661 gcgaaatcga aagcgcgctc agccagctgg cgggggtgaa acaagcaacc gttctggcga
     6721 tcggcgaaaa agaaaaaacg ctggcggcat acgttgttcc tcagggcgag gctttttgcg
     6781 ttaccgatca tcggaacccg gcactgccgc aggcgtggca cacgcttgcg ggaacgttgc
     6841 cctgttgcgc catctcgcca gagatctccg cagaacaggt agccgatttc cttcagcatc
     6901 gcctgctaaa actgaagccg ggtcacaccg ctggcgccga tcctctcccc ctgatgaact
     6961 cactcgctat ccagccgcgc tggcaggccg tggtggaacg ctggttagca tttctggtga
     7021 cacaacggcg actgaagccc gctgctgaag gttatcaggt ctgcgctggt gaagaacgcg
     7081 aggatgagca cccgcacttc agcggacatg atttaacgtt atcgcaaatt cttcgcggtg
     7141 cccgtaacga actgtcgtta ctgaacgacg cgcagtggtc gccggaaagc ctggccttta
     7201 accatccggc cagcgccccg tatattcagg aactggcgac aatttgccaa cagcttgcac
     7261 agcgcttaca gcgcccggtg cgcctgcttg aggtgggaac ccgcactggc cgcgccgcag
     7321 aatcgctgtt agcacagctc aacgccggac agattgagta tgtcgggctt gagcagagcc
     7381 aggagatgct gctgagcgcc cggcagaggc tcgccccctg gcctggcgcc cgtctgtccc
     7441 tctggaatgc agacacgctg gcggcgcacg ctcactcggc ggacattatc tggcttaata
     7501 acgccctgca tcgtctgctg ccggaagatc ccgggctcct tgcgacatta caacagcttg
     7561 ccgttcccgg cgcgctgctc tacgtgatgg agtttcgcca gttaacgccg tccgccctac
     7621 tcagcacgct cctgttaacc aatgggcagc cggaggcctt gctgcataac agcgccgact
     7681 gggcggcatt atttagcgcg gccgccttca actgtcagca tggcgatgag gtcgcggggt
     7741 tacaacgctt cctcgtacaa tgtcctgaca ggcaggtgcg ccgcgatccc cgtcaacttc
     7801 aggccgccct cgccgggcgt ctgccggggt ggatggtgcc gcaacggatc gtcttcctcg
     7861 acgccttacc gctgacggct aacgggaaaa ttgactacca ggcgctgaag cgtcgtcata
     7921 cccctgaagc ggaaaacccg gccgaagcgg atttacccca gggcgacatt gaaaaacagg
     7981 ttgccgccct ctggcagcaa ctcttatcaa ctggcaatgt caccagagaa accgacttct
     8041 tccagcaagg cggcgatagc ctgctggcga cccgtctgac cgggcaactt catcaggcag
     8101 gttatgaagc gcaattaagc gacctgttta atcatccccg gctggcggat tttgccgcca
     8161 cgctgcggaa aaccgacgtc ccggtcgaac aaccattcgt ccactcccct gaagatcgct
     8221 accagccctt tgcgcttacc gacgtgcagc aggcttacct ggtggggcgt cagccgggct
     8281 ttgccctggg cggcgtcggc tcacatttct ttgttgaatt tgaaattgcc gatctggatc
     8341 tcacccggtt ggagacggtc tggaaccgat taatcgcccg ccacgatatg ctacgcgccg
     8401 tcgtgcgtga tggacagcaa caggtgctcg aacagacgcc tcgctgggta atacccgcac
     8461 acatcctcca tacgcctgaa gaggcgttgc aggtgcgcga aaaactggcc catcaggtac
     8521 tcaaccccga agtctggccg gtattcgatc tccaggtcgg ttacgttgac gggatgcccg
     8581 cccgcctgtg gctgtgtctg gataacctgt tacttgacgg gctgagcatg cagattctgt
     8641 tggcggagct ggagcacggc taccgctacc cgcaacagct gcttccgccg ctgcccgtca
     8701 ccttcaggga ttatctgcaa caaccctcgc tacagtcgcc caatccagat tctctggcat
     8761 ggtggcaggc gcagcttgat gatattcctc cggcgcctgc gttgccgctg cgctgcttgc
     8821 ctcaggaggt tgaaacaccg cgcttcgccc gcctgaacgg cgcactggac agcacgcgct
     8881 ggcatcggct gaaaaaacgg gcggctgacg cccatctcac cccgtcggcc gtactgttgt
     8941 cggtgtggtc aacggttctc tctgcatgga gtgcacagcc tgagttcacg cttaacctta
     9001 cgcttttcga caggcgaccg ctgcacccgc aaatcaacca gattctgggc gatttcacct
     9061 cgctgatgct gctgagctgg catcccggcg aaagctggct gcacagcgcg cagtcactac
     9121 agcagcggct gagccagaac ctcaaccacc gcgatgtgtc agccatccgc gtgatgcgtc
     9181 aactggcgca acggcaaaac gtgcctgccg ttccgatgcc cgtcgtcttt accagcgcac
     9241 tgggctttga gcaggataac ttcctcgccc ggcgtaatct gctcaaaccg gtctggggca
     9301 tctcccagac gccgcaggtc tggctcgatc accagattta tgaatccgaa ggcgaactgc
     9361 gctttaactg ggattttgtc gccgcgctgt ttcctgccgg gcaggtggag cgccagtttg
     9421 aacagtattg cgcattgcta aaccgaatgg ccgaggatga aagcggctgg caactgccgc
     9481 tcgccgcgct ggtgcctccc gttaaacacg cagggcaatg cgcagagcgc tcaccgcgcg
     9541 tatgccctga gcactctcag ccacacattg cggcggacga gagcaccgtc agcctgattt
     9601 gcgacgcctt ccgcgaggtg gttggcgagt ctgtcacgcc cgcagaaaac ttctttgagg
     9661 cgggcgcaac gtcgctgaat ctggtgcaac tgcacgtttt gttacaacgt cacgaatttt
     9721 ccaccctgac gttgcttgac ctcttcaccc acccttctcc tgctgccctg gccgattatc
     9781 tggccggcgt cgccacggtg gagaaaacaa aacgacctcg ccctgttcgc cgtcgtcagc
     9841 ggcggatata gcgcgaagca aactgatttt ccccggaacg ccatcgcgaa cgcatggcgt
     9901 tccattgact ttgtgaatct taggaaacgg gaccgattat ggataacttg cgcttctctt
     9961 ctgcgccgac agcagattcc attgatgcat cgatcgttca acactacccg gactgcgaac
    10021 ctgtcgcggt tatcggctac gcctgccatt ttcctgaatc gccggatggc gaaacgttct
    10081 ggcaaaatct gctggaaggt cgtgaatgca gccgacgctt tacgcgcgaa gagcttctgg
    10141 ccgtcggtct ggatgccgcc atcattgacg atcctcatta tgtcaatatc ggtacggtgt
    10201 tagacaacgc cgactgcttc gacgccaccc tgtttggcta ttcgcgacag gaagcggagt
    10261 cgatggaccc gcagcagcgc ctgtttttgc aggcggtctg gcatgcgctg gaacatgccg
    10321 gttatgcccc cggcgccgtc ccccataaga ccggcgtttt cgcctcttcc cggatgagta
    10381 cctaccccgg tcgcgaagca ttgaacgtga cagaagtcgc gcaggtaaaa ggtctgcaat
    10441 ctctgatggg caatgataaa gactatattg ccacccgcgc cgcgtacaaa ctcaacctgc
    10501 acggcccggc gttatcggta cagaccgcct gctccagctc gctggttgcc gtgcatctgg
    10561 cctgtgaaag cctgcgcgca ggcgaatccg atatggcggt tgccggcggc gtggcgctct
    10621 ctttccccca gcaggcaggc taccgctacc agcccggaat gattttctct cctgatggtc
    10681 actgtcgtcc ctttgacgcc tcggctgagg gcacctgggc cggtaacggt ctcggctgcg
    10741 tggtgctgcg tcgcctgaga gacgcgctgc tgtcaggcga tccgattatc tcggtgatcc
    10801 tctccagcgc ggtcaacaac gacggcaaca gaaaggtcgg ctataccgcc ccttccgtcg
    10861 cagggcaaca ggcagtcatc gaagaggcgt taatgctggc ggccatcgac gacaggcagg
    10921 taggttacat tgaaacccac ggcaccggca caccgctggg cgacgcgatt gaaattgaag
    10981 cgttacgcaa cgtctatgcg cctcgcccgc aggatcagcg ctgtgcgctc ggttccgtga
    11041 aaagtaacat gggccatctg gataccgcgg cgggcattgc cggactgctg aaaaccgttc
    11101 tggcagtcag tcgcgggcaa attcctccct tactgaattt tcacaccccc aacccggcgc
    11161 tgaaacttga agagagcccc tttaccatac cggtgtcggc acaggcatgg caggacgaaa
    11221 tgcgctatgc gggcgtctcc tcctttggta ttggcggcac caactgccat atgatcgtcg
    11281 cctcgctgcc cgacgcgctc aacgcgcgcc tccccaatac ggatagcggc agaaaaagta
    11341 ccgcgctgct gctcagcgcc gccagcgaca gcgcgttgcg gcggctggcg acggattatg
    11401 ccggggcgct gagagagaat gcggatgcca gctctctggc cttcacagcc ctgcacgcgc
    11461 gccgtctcga tctccccttc cgcctggcgg cgccattaaa ccgtgaaacc gccgaggcgc
    11521 tcagcgcctg ggccggtgag aaatcggggg cgctggttta cagcggccac ggcgccagcg
    11581 gcaagcaggt gtggctgttt accggccagg gctcgcactg gcgcactatg ggtcaaacga
    11641 tgtaccatca ctcaacggcg tttgccgaca cgctggatcg ctgtttttcc gcctgtagcg
    11701 aaatgctcac gccgtcactg cgcgaagcga tgtttaaccc cgattcggcg cagctggaca
    11761 atatggcctg ggcgcagccg gcgattgtcg cgtttgaaat cgcgatggcg gcgcactggc
    11821 gtgctgaagg actgaagcca gacttcgcca ttgggcattc cgtcggtgaa tttgccgctg
    11881 ccgttgtctg cggacactat acgattgaac aggtcatgcc actggtttgt cggcgcggcg
    11941 cgctaatgca gcagtgcgca agcggcgcga tggtggcggt atttgcagac gaagacacgc
    12001 tgatgccgct ggctcgccag tttgagctgg atctcgccgc caacaacggt acgcaacata
    12061 cggtattttc cgggccggaa gcccgtctcg cggtattttg cgccacgctc tcgcagcatg
    12121 acattaacta tcgtcgcctg agcgtaaccg gtgcggcgca ctccgcttta ctggagccga
    12181 tactcgatcg gttccaggac gcctgcgcgg gactgcacgc ggagccgggg caaataccga
    12241 ttatttccac gctcaccgcc gacgtcattg atgagtcaac gctcaaccag gcggattact
    12301 ggcgccgaca catgcgccag ccggtgcgtt ttatccagag tattcaggtg gcgcatcagc
    12361 tcggcgcccg cgtttttctg gagatggggc ccgatgccca gttggttgct tgcgggcagc
    12421 gcgaataccg cgataacgca tactggatag ccagcgcccg gcgtaacaaa gaggcgagcg
    12481 atgtcctcaa tcaggccctg ctccagcttt acgctgccgg cgtcgcccta ccgtgggccg
    12541 acctgctggc gggcgatgga caacgtatcg ctgcgccatg ttatccgttt gatactgaac
    12601 gttactggaa agagcgcgtc tccccggcct gcgagcctgc cgacgcagcg ctgtctgccg
    12661 ggctggaggt ggcgagtcgc gccgcgacag cgctcgatct ccctcgcctg gaagcgctta
    12721 aacagtgcgc cacgcgactg cacgccatct acgtcgatca actggtacaa cgctgtaccg
    12781 gcgatgccat tgagaacggc gtggacgcca tgaccatcat gcgccgtgga cgtctgctgc
    12841 cccgctacca gcagctactc cagcgcctgc tgaataactg cgtggtcgac ggcgattacc
    12901 gctgcaccga cgggcgatac gtccgcgccc gccccattga acatcaacag cgggaatcac
    12961 tgctgacgga acttgccggt tattgtgaag gttttcaggc tattcccgac accatcgccc
    13021 gtgccggcga tcggttatat gaaatgatga gcggcgcgga agaaccggtg gcgattatct
    13081 tcccgcaaag cgcctccgac ggcgtggaag tgctgtatca ggaattcagc tttggccgct
    13141 atttcaacca aatcgccgcc ggggtattac gcggcattgt ccagacgcgt cagccccgcc
    13201 agccgttgcg tattcttgaa gttggcggcg gaaccggcgg caccaccgcg tggctgctgc
    13261 cggaactcaa cggcgttccg gcactggagt accatttcac cgatatctcg gcgctgttca
    13321 cccgtcgcgc ccagcagaaa ttcgccgact acgattttgt gaagtatagc gagctggatc
    13381 tcgaaaaaga ggcgcagtct cagggtttcc aggcacagtc ttacgatctt atcgtggcag
    13441 cgaacgtgat tcacgccacc cgccatattg gccgcacgct cgataatctg cgccccctgc
    13501 tcaagccggg cgggcgcctg ctgatgcgcg aaatcaccca gccaatgcgt ctgtttgact
    13561 tcgttttcgg cccgctggtt cttccgctac aggatctcga cgcccgcgaa ggtgagttat
    13621 tcctcaccac cgctcagtgg caacaacagt gccgccacgc cggattcagc aaagtggcgt
    13681 ggctaccgca ggatggcagc ccgaacgccg ggatgagcga acatatcatt ctcgccacgc
    13741 tgcccggtca ggcggttagc gccgtaacat tcaccgcgcc atcagaaccc gtgttggggc
    13801 aggcgctgac ggataacggt gattatctcg ccgactggtc tgattgcgca ggtcagcccg
    13861 aacagtttaa cgcccgctgg caggaggcat ggcgtctgct ttcacagcgt catggcgacg
    13921 ctctgcctgt ggaaccgccc cccgtcgccg ccccggagtg gctggggaag gttcgcttaa
    13981 gctggcaaaa cgaagccttt tcccgcggtc agatgcgcgt tgaagcccgt catcctgctg
    14041 gcgagtggct gccgctatcg cccgccgcgc ctcttcctgc gccgcaaacg cattatcaat
    14101 ggcgctggac gcccctcaac gtcgccagca ttgaccatcc gcttaccttt agcttcagcg
    14161 ccggtacgct tgcgcgcagc gacgagctgg cgcaatacgg catcattcac gatccgcacg
    14221 cctcttcacg actgatgatt gttgaggaga gcgaggatac gctggcctta gcggagaaag
    14281 tgatagcagc gctcaccgcc agcgcagccg gattgattgt ggttactcgc cgcgcgtggc
    14341 gagtcgagga aaatgaagca ctctctgcat cccatcacgc gctatgggcc ttgcttcgcg
    14401 tcgcggccaa cgaacagccg gaacggttgc ttgccgccat cgatctcgcc gaaaacaccc
    14461 cgtgggaaac gctgcatcaa gggttgagcg cagtctcact atcacagcgc tggctcgccg
    14521 cacggggtga caccctttgg cttccttcac tgtcgcccaa tacgggatgc gccgctgaat
    14581 taccggcaaa cgtgtttacc ggcgatagcc gctggcatct ggtgaccgga gcgtttggcg
    14641 gattaggccg ccttgccgtg aactggctca gagaaaaagg ggcgcgacgc atcgccctgc
    14701 tggcgccgcg cgtggatgag tcatggctac gcgacgtgga gggcgggcag acgcgcgtct
    14761 gccgttgtga tgtgggcgat gccgggcaac tggccacggt tcttgacgat ctggcggcca
    14821 acggcggcat tgccggagcg attcatgccg ctggcgtatt ggctgacgcg cccttgcagg
    14881 agcttgatga ccaccagctg gctgccgttt tcgcggtaaa agcgcaggcg gcaagccagc
    14941 tgttgcaaac cctgcgcaac cacgacggac gctatcttat tctctactct tccgctgccg
    15001 ccaccctcgg cgcgccgggt c
//
LOCUS       DAYPHN010000120        14854 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000120 DAYPHN010000000
VERSION     DAYPHN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.120"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            106..1191
                     /locus_tag="ACG0BW_003417"
     CDS             106..1191
                     /locus_tag="ACG0BW_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFK7260342.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDYVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            1421..3337
                     /locus_tag="ACG0BW_003418"
     CDS             1421..3337
                     /locus_tag="ACG0BW_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFK7260343.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRAHNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVFAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            3327..4070
                     /locus_tag="ACG0BW_003419"
     CDS             3327..4070
                     /locus_tag="ACG0BW_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260344.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAADKRVTQENEYSDFA
                     NYPQPTLQTAIVANSLEARLLENPIWGTW"
     gene            4080..4649
                     /locus_tag="ACG0BW_003420"
     CDS             4080..4649
                     /locus_tag="ACG0BW_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260345.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            4723..7038
                     /gene="nrdA"
                     /locus_tag="ACG0BW_003421"
     CDS             4723..7038
                     /gene="nrdA"
                     /locus_tag="ACG0BW_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFK7260346.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLAQAVAVEPEDECDGACKI"
     gene            7145..8287
                     /gene="nrdB"
                     /locus_tag="ACG0BW_003422"
     CDS             7145..8287
                     /gene="nrdB"
                     /locus_tag="ACG0BW_003422"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFK7260347.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCECQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKSLFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            8365..9234
                     /locus_tag="ACG0BW_003423"
     CDS             8365..9234
                     /locus_tag="ACG0BW_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFK7260348.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLEKKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADAEERRHAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            9406..10509
                     /locus_tag="ACG0BW_003424"
     CDS             9406..10509
                     /locus_tag="ACG0BW_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFK7260349.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTETELDKLGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMQRQAPEHSDPVVI
                     FPNEWSELDDFKWDEILILGYAPLPWIKVPVAV"
     gene            10511..10924
                     /locus_tag="ACG0BW_003425"
     CDS             10511..10924
                     /locus_tag="ACG0BW_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260350.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVSDSDAGQFIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNSTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            10921..11397
                     /locus_tag="ACG0BW_003426"
     CDS             10921..11397
                     /locus_tag="ACG0BW_003426"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFK7260351.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            11397..12041
                     /locus_tag="ACG0BW_003427"
     CDS             11397..12041
                     /locus_tag="ACG0BW_003427"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFK7260352.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGDSMPLYERLQIQMEIAKHIELLTRGAEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            12103..12522
                     /locus_tag="ACG0BW_003428"
     CDS             12103..12522
                     /locus_tag="ACG0BW_003428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260353.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGTQVGEAINIAMSQFSEGSKKSLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TVSGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            12532..13089
                     /locus_tag="ACG0BW_003429"
     CDS             12532..13089
                     /locus_tag="ACG0BW_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFK7260354.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSVWVAHNGEAFDIPF
                     IRHEFSGYGVRLPEVPVIDTMLSGLWATEDGKRPRLEELAFSLGYIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            13248..14057
                     /locus_tag="ACG0BW_003430"
     CDS             13248..14057
                     /locus_tag="ACG0BW_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260355.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESVEAAENEPEQL
                     GFIEVEECVEVNDELKVQQSNDSNTNKKARTARGPRFTLSDKDDSFFNKAGLEKDIFL
                     DAYDNAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            14241..14834
                     /locus_tag="ACG0BW_003431"
     CDS             14241..14834
                     /locus_tag="ACG0BW_003431"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFK7260356.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
BASE COUNT         4104 a         3183 c         3662 g         3905 t
ORIGIN      
        1 gtatattaag acgctcgttt cattcctaaa ataccgttat gcatagttgt ttaccttctc
       61 attgctctta aaatttgtat caaaataacc acaaaggaaa aacacatgac tttgccatac
      121 ggcgtcattt ctgactgcca ctaccacaaa tgggatgcgt tctccacgac gaacgcagag
      181 gggcttaact ccagacttga aatacagttg gaagcaacga aagaagcagc catcgccatg
      241 aagaaggccg gttgtaagta catgttggtt gccggtgata catttcacgt ccgaggaact
      301 gtgtcccctt ctgttttgca ttacgtaact gaaacgtaca agtggattat caacgagctt
      361 gatctgacag tagtaatgct ggccggtaat cacgatcttg aaaccaacga ttcagtatat
      421 agcgccaacg cagcagcatc gctgagttct atcggcgtgg taatcgtatg tggcaaacgc
      481 ccacactcaa taaaaattgg tgatgtgact gtccacctga ttagctggcg taacaatcat
      541 gcggagctta tcagcgatct gaaagcatta cgtaagagcg tagaaggtga taatcatgac
      601 gttgttatcc atacatccat taacaaagcc attccaacaa tgcctgacgt cggtatcgat
      661 gcgcaggagt taaaggatat cggctttcgt ctcgtgctta gtgggcatta ccacaaccac
      721 aaagaggtca ttcctggagt tatcagtgtc ggtgcgctga cccatcaaaa ttggggagat
      781 gttggatctc tggctggtta catgatcgta aacccggacg gcagtttcag tcactacgaa
      841 accagtgcgc ctaaattcat taacctggaa gattatgttg ccgatgacca aattcgcggc
      901 aactacgtgc gttttcgcgc cgtaattgag aacgatgaag aaggcattaa gtaccagaac
      961 atcctcaaaa caatgggtgc aaaaggtgtc gtgtgcaact tcatccgtaa gtcatcaatg
     1021 atggaaggga cagcaagcac aacggaaacc agcaaaatcg atagcctggg agagtcggta
     1081 tctgcttatt gcaagattgt ccacgatact gacggcggat ttgatctgag caagttggat
     1141 attttgtgtc aggaaatcct caccgaagcg gagagttcgg aggctgtgtg aggcaaagtc
     1201 gttatgggag ctttcgagac tttgccatca cgatgaaaag acttgaacga ggccagacgg
     1261 tgatgtttca caagccctac ccgccacaag gaaatcccgt agcgttttat cttggaaggt
     1321 taaccagaaa aggcgtattg aggcgcagat ccttcccggc gcatacggag ttcagattga
     1381 aagaaggtca aaagctaaca cacggtatca aaggtgttat atgaagtttt taaagctcca
     1441 ggttgagaat tttatggcta tcgccagcgc ggaggtcgag ttagatcagc gtggtttagt
     1501 actcattcag ggtgttaata gtgatgatag ttccgcatca agtaatggct ctggaaagtc
     1561 aacgctaatg aatagcctga tgtggtgtct ttatggcgaa acagctcatg gtgtgaaggg
     1621 tgacgatgtg ttgtctaccg accatgaaaa gaactgtcgt gttgcagtaa ccatcgagga
     1681 tgaaggcaag agatatgcga tcattcgtca ccgtaaacac aaagagttca aaaatcgtct
     1741 tatcgttcgt ggtgaagatg gcgatatgac gaaaggcaaa gatgcgctga cgcaagagtt
     1801 cgtcgagcgt ctgatcggtg catctaaaga ggttttcatg gcttccatct atgcgagcca
     1861 agaagctatg ccagatttac ctggaatgtc cgacaaaaac ctcaaaacca tcgtagaaga
     1921 agccgctggc gttgacagac tgacacgcgc ctacgctatt gctcgtgagc gagctaatgc
     1981 agctgccgca cgtatggatg tggttaaaac caaattggag tcgacaatct cgaccattga
     2041 ggcaacacag tcagaaattg agtcagcgaa agcctcctct gaatcatggg agcaagagcg
     2101 ttctaaacgt tatgacgatg ccctggctgg gctggccagt gccgaagttg agttaacgga
     2161 agttgaactt gagatccgca ctcttcccga acagatccgt gataccgaga aggcaatcga
     2221 aagtgagcgc aaaaagttag cctcaaaaga agaacatgac gccaagttgc tcaaagttcg
     2281 tggtgcgata actgatattc gggcaagcat caaagctaca gaaaatagtc aggctgatgc
     2341 aatgaaccgc gcgcataatt ttaagactaa agcagaagag gttggtacta aagtgggatc
     2401 accatgccct acttgtggca aagcctactg cgaagaagat ctatcaacgg tgaaggagaa
     2461 tttcattgaa caagcacgtc aggaaattgg tcaggcgaag acacttgcag aggcaatggc
     2521 taaacacaaa acgaatcttg agaaagcgtt aagcattgag tctgcccttg ttaaaacgac
     2581 acctgatgta acggctatca ttgcccggat tgaagagctt acgaaacaac tctcatcttt
     2641 gcgtcatcgt gagaaggagg tttttgctat tgagtctctt gtgactcgtg ctcgtactga
     2701 ggtcgatcgt atatcaaaag agattaatcc gtttattgct cttatcgcca gacacgaaga
     2761 taacctggta tccagtaagt ctaccttcaa gtccttaaaa gatgagttga aggctattca
     2821 ggaacaaacg ttgctattgg aaaaagctcg tcaggtctac tctcctgccg gggtgcgttc
     2881 tcatattttg acgtctgtta cgcctttcct gaatacacgc actgccgagt atctcaatac
     2941 gttgtctgac gggaatatta ctgctgagtg gtcgacgatg gatgtcacta aaaaaggtga
     3001 gtatcgcgac aaattcaaca ttagtgtgca gaagaaaggt tcaagtaagt cgttccagac
     3061 cctctctggt ggtgagaagc ggaaggttcg cattgcgtgt tctttggcat tgcaggatct
     3121 ggttagtaac cgggcgagta aaaacatcga tttgtttatc ggcgacgaaa ttgacgatgc
     3181 actcgataca gccggtcttg aacgcctcat gggtattttg gagtccaaag ctcgcgagcg
     3241 aggtactgtg ctgattatct cccataaaga gatgaagtca tggttccggg aaactattac
     3301 gctggaagtt aaagaggggc gcagctatgt cgtttaaatt aagccgctcg cagtttttgc
     3361 aggtatttgc agtgatgcag tcgataaaac tgatcaatgg gcatacttcc aatggtgcgg
     3421 ctccacgtat tctgtggggc agcaacaata ttgacggagt acaattcgcc gcgttgcttg
     3481 gtctaatatc cgagacgcca ttgatgcaaa gtttgaaatc actaccacct ggatgtattg
     3541 cgccgatcct gattaatcct tttgttgagg ggggatatct tcccaacgtc gggcctgggt
     3601 ttattgcatc ccatgaaact gaagatctta acattaatag cgaagggttc tttgggggaa
     3661 tgggtgcgca tcactgtatg gctttcacga accttattcg acttgccaat aagcgggtgg
     3721 atagtttggc atcgccaggt gatgctttta ctggtttcct tatccaaagg agggataaaa
     3781 agtacagtgc ggacaaacta cagtttgttg gtaagtatgg agaaatggta gaaatcgaac
     3841 ttcagctccc tcatgtttta gcaaacgata gtgcagacag tcggaggctg ttgggcatca
     3901 tgcgtcattt catagcaagt ggtgttaaac atgccgcaga taaacgtgtc acgcaggaaa
     3961 atgagtattc agactttgca aactatcccc aaccaacgtt gcaaacggca atagtagcca
     4021 attcgttgga ggcgagatta ttggaaaacc ctatatgggg aacatggtaa ggagactata
     4081 tgagtaaaaa aatcagcgta gttggtgttg atccctcaat gagcaacttt gggcttgctg
     4141 tgggcacttt agaccttgag acggacgaac ttgagattca cggccttact cttgttgaga
     4201 ctaaagcggg gagtaacaaa aagaccgttc gtgtgaacag tgacgatctg cgccgagcca
     4261 gtgaaatatg gcgtgttgcg aagccaatca ttgataaggc aaatatggtt ttttgtgagc
     4321 taccggttgg gagccaaaac tctcgttcgc agacgtctta cggtatttgt atcggtgtac
     4381 ttgcgtgtgt ggataagcca ttgatccagg ttactccaaa cgaaatcaag cattttgtcg
     4441 gcaataaact tactacatcg aaagaagaga ttatccagtg ggctacgaaa aaacacccta
     4501 aagcaccgtg gctgcgtcgt aagcaatctg gacaggatgt tctcgtgaac aaaaacgaac
     4561 atttggctga tgcggtggct gccatccata ccggtatgca aacagatcag ttccgccagg
     4621 tgcgcgatgt tcttaagtct ctcatttgat ttcattgata ggtaagtgct tatctattaa
     4681 catgggccac tatatttagt ggccctcttt atttggtgat acatgataag catcgtaaaa
     4741 cgtaacggcc aaacagagcc gttatccgaa gaaaaataca accgcgtcgt aatgtatggc
     4801 gtagaaggca ttcgtggtgt aagcgcatcc gctgtagcaa tgggagctgc ggccagcatt
     4861 tttgatggga tgaccaccag ccagttgcat gaggctttgg ttaaatctgc cgctgatttg
     4921 atctcaccag aagcaccaaa ttactcacag gtggctgccc gcctgaacat ttttaaaatc
     4981 cgcaaagatg ccttcggtcg ttacgactat ccgaacttct accaacacat tgtcaagaac
     5041 gttaacaagg gcgtttatga caaggatttg ctgacacatt attcgtttga agagatcgaa
     5101 gaactcggca attacattaa gccgaaacgt gacgatcttt ttggctatgc agctacggtg
     5161 cagttgcaaa gcaaatacct cgttcaaaac cgtgttactg gtgagattca tgaggggccg
     5221 caacatatct atatgctggt aggcatgtgt ctgttccaga attgggaaga cgactgcgct
     5281 ggcaaaacac gtatggagat ggtcaaaggt ttctatgacg ttacaagtac gttcaaactg
     5341 tctctgccca caccaatcat ggccggcgtc cgtactccaa cccgtcagtt ctccagttgt
     5401 gtgctgattg agtctggcga tagtctgaaa gggattagtg cagcttcagc cgcaattatc
     5461 gactacgttt cacgtcgtgc tggaattggt attggttttg gccgtatccg tgcgctgggc
     5521 agcgagatcc gcaatggtga agccacccat accggagtta ttccattcct gaagcatttt
     5581 cagacggctg ttaaatcttg ttcgcaaggt ggtgttcgtg gtggcgcagc aacagcgttt
     5641 tacccgatct ggcatcttga agttgaaagt ctgctggtgg tgaaaaataa ccgtggtatt
     5701 gatgaaaacc gcgttcgcca tcttgattac ggcgtcatga gtaaccgtct aatgtaccgt
     5761 cgactcgtca gaagcgagaa catcactctg ttcagcccgc atgatgtgcc tgatatgtac
     5821 gaagccttct tcacagacca ggagctgttt gaaaagctgt accataaata cgaagccgat
     5881 gattcaattc gcaagaagtc agtacctgcc attgagctgt tctcatctct gatgcaggaa
     5941 cgagcgtcca cgggccgaat ttatattgcg aacgtcgatc atattaatga gcatggcgct
     6001 ttcattcctg ctcttgcacc tgtccgccag tcaaacctgt gtatggagat caccctaccc
     6061 acccgtccac tggcatttac cgacgacccg aacggtgaga tcgcgctatg cactttatcc
     6121 gcttttaacc tcggagccat ccgttcactg gagtctctta aagaggtggc gttctatgcc
     6181 gttgctgcac tggattcgtt actggattat caagactatc cgatggaggc agccgaagtg
     6241 cctgccaaag ctcgtcgtag cttgggaatt ggtgtaacca actttgctta ttacctggca
     6301 aagaatggcg ttcgttattc tgataccgct ggcaataaac tggtgcatga aacattcgaa
     6361 gctatccagt attaccttct tgatgccagc tgccgacttg ctgaagcaaa aggtgagtgt
     6421 gactggtttg agcagaccaa gtacgcaatt ggtcagttgc cgatcgacca ttaccgttct
     6481 tcattagacg aaagtggcga aaccaacttt gagttaaaga tgccgtggga agaactgcgt
     6541 gaacgtattg caaaatacgg ccttcgcaac tccacactga cggcacaaat gccatgcgag
     6601 acttccagcc agatcactaa ttccaccaac ggcatcgaac cgcctcgtgg cccggtgtca
     6661 gtgaaatctt ctaaggacgg catcgtcaag atggtcgtgc ctgagtttga aaaactgaag
     6721 gaacagtatg aatacctgtg ggatatgccg gacaaccgcg gctatctgac aaaggtggcg
     6781 atcatccaga agttctttga ccaggctatt tcggccaata ccaactatga cccttctcgc
     6841 tttgaaggcg ataaagtccc aatgatgacg ctactgtcag atttgcttct cgcctacaag
     6901 atgggagtta aaacgcttta ctaccacaac accagagatg gtgcaggaaa gcgtgatgac
     6961 gacgaaccgc agaatccact ggcgcaagct gtagccgtcg agccagaaga tgagtgcgac
     7021 ggagcctgca aaatctgaca tatggtgggg gatatcccca ccttctcttt gatttgtaag
     7081 ccttgtttaa acacataaga taacaacttg tttaaacgca ccaaaaaaga aaaaggaaaa
     7141 acacatgtca tattcaacgt tccgtttggg tgctaatgat gcaaccaaag agcctatgtt
     7201 cctcggacaa tctgtcaacg tggcacgtta cgatcagcaa aaataccgtg attttgaaaa
     7261 gttgattgaa cgtcaattgt ctttcttctg gcggccggaa gaagttgata tttcgagcga
     7321 tcgtatcgac ttcaacacga agctgcggga ccacgaacgt cacatttttc tgagcaatct
     7381 ccgttatcaa acgttactcg attcagttca gggacgtagt ccaaatgcaa cgctgctgcc
     7441 gcttatctct attcctgaac tggaaacgtg ggttgaaacg tggtctttct ctgagactat
     7501 ccatagccgc agctacaccc acattattcg tggcatggtg gacgatccga gcattgtttt
     7561 tgacggtatt gttacggatg aagaaatcat caaccgagcg atcagtatct ctgctgaata
     7621 tgacaggctt tatgggatga cctgcgagtg ccagtcgtta ggtgagaagg aatttgagcg
     7681 tctgtacgta aatgaatatg gctgggagcc ataccctttg catcgtcagc ttttccgcac
     7741 gttggtgtcc attaatgctc ttgaggcgat ccgtttctac gtaagttttg catgtacgtt
     7801 tgcctttggt gaacggaagt tgcttgaggg taacaccaaa attatgcgct ttattgcccg
     7861 tgatgaagct ctgcattgcg aaggaactga acgcatgatc cgcttcatgc gtaccggtcg
     7921 cgaaggttta ttgtggaaag agattgctgc tgatgaagaa aacgtcattt acgacaccat
     7981 gaaatcagtc gccgaacaag aaatgaactg ggcagactat ctcttcaaag acggttcgat
     8041 gattggttta aacgcggata ttctcaaaac ctatgtaaaa taccgcacca atctggctat
     8101 gaatcgtctt ggcctgaagt ctttatttcc agaagttacc actgatccgc tggtctggat
     8161 gaacaagtgg ttgttaaccg acacactgca aattgcacca caagaggcag agcaaagcac
     8221 atatctggta ggtcagatcg attctaccgt ggataaggct tctctaagcc agtttgcaga
     8281 cctgtaaacc gatacaaagc attatgtggc ctagcaacgc tgggccacaa tggatcacaa
     8341 gaattaagaa ggaacaaaac tagcatgaac tttaccaaac tgactgacca cctgaaactt
     8401 gccaccgatc gactcattgg atttaagcca gaaccatatg aattgcatga aggccatggt
     8461 gtagccactg aaagtattta caagatggtc gatcagtttc atgaactctt ccagcatccg
     8521 agacgcgtta tgccgacacc agagctgctt cgtctccgtg caagcctgat tcatgaagaa
     8581 gctgtagtgg aaggtattcc agctgcaatg aatggggata ttgagcaact gctggatgca
     8641 atggccgact ttttatacgt tggtgttggt acgatggtcg ccatcaaagg tggtatttct
     8701 accggcatga cctattacac gcaggaacag agcattgatc gctttatgca gacaattttt
     8761 gtgcctggta acactgtttt cgatgatatg gcaatgccat ttcaggaagc tcgtgaggcg
     8821 tcatgtatgc tcgaagagct ggcagataaa cttgagaaga agactgttaa ggattctgag
     8881 ctgattcagg aactgcgccg tgtaatgaac aaaatctatg tggcgtgcat gatgacttat
     8941 cgactggctg attttctcgg tatcaatgtc gtcgagctgg ttggcgaaat tcatcggtcc
     9001 aacatgacaa aattatggcc tgcagatgcc gaagagcgtc gccatgctgt ggccaactgc
     9061 aaatacgact cttctgacct gggatttcgc catgctgatg gcaccgataa gatgatcggt
     9121 tttcgaattt ccgatggaaa gattctgaag tctccaacct atagtgatgt cgatttatcc
     9181 tcctttgttg agcaagctaa agcctcagca atgtacggaa tgatcaaaaa ataattgtag
     9241 gtagttatct atctgtgtat attgcgctgg cgcgttaaat ttctgaaaca actattcgtt
     9301 tttggtggcc tatggccacc atttttttat ctgtctggtc ttgttctctc aataaatgta
     9361 aactcacgca atgaataagt ggttacttat ctttgtgagg tttttgtgtc actccttttg
     9421 aatcgtgagc atacgaacgg tcaggtaaca aacgcatcgt atgcaaaagt tattgagacg
     9481 gtgcttaaaa gcggcgtgca ggctgatgat cgcacaggca ctggtacttt aagcacctgc
     9541 tacgttccct cttactacat gcttactggt gggactgtgc cgcttatttc tggaaaggcg
     9601 gtaaatctta agccactgct tgtcgaactt gagtggtatc tgaaaggcac gggcaacatc
     9661 caatttctca aggataacgg cgttaagatt tgggatgcat gggccgatga gaatggcgat
     9721 ttggggccgg tttacggtaa gcagtggcgt cgatgggaag atacccgcat cgtgagccat
     9781 agtgaatatc tgagcaagat cgctactttc cgtgaacgcg ggtacaaagt cgagggatac
     9841 ctgggtatca gtgaagatcg cgtagtgctg tcccgtgaaa tcgatcagct acagcgtatt
     9901 gtcgatacac tgcgcacgaa ccctaccgat cgtcgcatca tgcttaacgc atggaacgta
     9961 ggcgagcttg aggatatgaa actgccacct tgccactttg tcttctcttt gtggagtcgt
    10021 gaactggatt ttgaaacccg tttaacgatg gcaactgaca ttggtctaca acacagtcgc
    10081 ctcggttatg agtctatcta caccaagatg ctatacgatc tggagatgga cggcagtgtt
    10141 actgaaactg aactggataa acttggaatc cccaaacgca tcctcaactc ctgcctcgta
    10201 cagcgtagcg tagacacttt tgttggtatg ccattcaata ttgctggcta tggcattctc
    10261 actcattttc tcgcgaagat tacgggtcac atggccggtg catttgtgca ttttggcttt
    10321 gacgtgcatt tgtacaacaa ccacatggaa ggtgtgtgtg agctaatgca acgacaggct
    10381 ccagagcatt cagatccggt cgttatattc cctaatgaat ggtctgagct ggatgatttc
    10441 aaatgggacg agattcttat tcttggctat gcccctctac cgtggatcaa ggttccagtg
    10501 gcggtgtgat atggcaagag gtatgtatgt cttatgcgaa attgaaggtg tgctggcaaa
    10561 taccagccat cgtaaatctg tatctgactc ggatgcaggc caattcattg ccggtgatga
    10621 actcattttc cccaccagcc gtatgttgcg tggttttgct cgctcagggg ctgaagtggt
    10681 gcttatcagt agccgctctg aaactcttga agcgcccact aaacgatggc tgaaagattt
    10741 tggtgttgat tacgactggc ttcacctcgt accgaatagc actagttatg agaagcatat
    10801 taagcgcaca ttagcggagc ataaaggcga tctgcttatc gctgcgctgg tgcacgatcc
    10861 tcgactccgt gccgctttag ctgactcaca tcatagaccg gtcatctatg aggtgagcaa
    10921 atgaagatga tagctgctgt tggccgtaac tatgagatcg gcatagcgaa cgaactgccc
    10981 tggcgttgtt ctaccgatct gaagctattt aagagactca ccaaaaacgc cactgtcgtt
    11041 atggggcgta aaacgatgga aagtctcaaa cgccctcttc cagagcgtca taacctcgtt
    11101 ttgacgcgct ctcatggctt tgtaccaaat ggattctacc ctgctggtgt ggatgatgtg
    11161 ttgcgattac cagagcctgt gtgggtgatt ggcggggaac aaatttactc gctattcatg
    11221 ccgcatgttg aagagatttg gctctcccac atcggcgttg atgtgccaaa cgccgatgca
    11281 ttcttcccgg caagcatgat gcgtaattta ggctttgtgc ctgttgaaac agcttatacc
    11341 caacgagcca gcgaggaaga gcctggcttt tcgcagatcg tatacagaag gtcgtaatgg
    11401 attaccggat tgggatcact ggtgctcagg gcagtgggaa aacaaccctg gctaaatata
    11461 tcgacaaaca ttacggaatc ccttacgtgg atgctggtgt cggaagtttg atgagccgcc
    11521 tcggtgttcg agtaggtgat tctatgcctc tatatgagcg gcttcagatt caaatggaaa
    11581 tagcaaagca tatagagcta cttacgcgtg gtgctgaagg ctttgttatc gatcgcacac
    11641 ctgctgatgt tatggcctac acgttggatt tggtcggcca tacaaatgaa gatcggtgta
    11701 ttgagttagc cctcgatatc gaaaagtttt gccacaaaac tgctatttca aactttaacg
    11761 ccattgctgg cctacgcccg ggggtcgctc tctcagagcg agattacttg cggtcacaac
    11821 gagcatcatt agaccgtctg tatgtcgctc gtattgatgc gttgatgtgc ggggaactga
    11881 caaaaattca cctgcatccg caaaggggag atctgcaaac cttcgtcgtt tccaaccggt
    11941 atcgcacagt tgaagcaaga gccagatcag tgatgagaat gctagataac gcggtagaaa
    12001 agatagaaaa ccggttctgt ggccgagtga ccgttcatta gaaattgttc gcctcttcga
    12061 cattgcgaca ataaaactct caaaatgggt taaggataaa aaatgtttag tgaaatgttg
    12121 cttgaagatg aactggatcg gaaaacaaca gaggctttga ttcgtgtagc ggacgaacat
    12181 tcccggtcgc ttatgagcga tcgagaggct cgtctggcta ttcgtgccat attcgaaact
    12241 gcgcaggggc ttgttggtac acaagtgggt gaagccatta acatcgccat gtctcagttc
    12301 agtgaaggca gtaaaaagtc tctgtttcct atgcatttga tgctggctgg tggcacggtg
    12361 ctttatatct ctgtttgtct ggatagcaac caaatcaata ttctcaacac tgtgtcaggt
    12421 aagtggaaag atccgattgt ctgtgaaacc agtgaagaaa ctttgaaaaa agcggctcaa
    12481 tttgtacgta gcgcactact taagggcgct aagaagttgt aaggagttct gatgacaacg
    12541 attgttgcag gcatcgatat cgagtctacg ggactggatt tccttgctgg tcataaaatt
    12601 attgaaatcg caattacccg ctatgaactg gagacacaga gacatattga tagtctggag
    12661 atgcgtttta accctcgcag aaacatagat ccgaaagctc aagccgttca tggcatttca
    12721 ttggaacagc tcgcagctga acctttgttg tcaaatcatg ccagcgaaat tggcgcttat
    12781 atgggggcat gtagtgtgtg ggttgctcat aacggcgaag catttgatat accatttatt
    12841 cgacacgagt tttcagggta tggagtaaga ctgccagaag ttcctgttat agatactatg
    12901 ttatcgggat tgtgggccac agaagacggc aaacgtcccc gccttgaaga gttggccttc
    12961 tctcttggct atatatacga tcatgccaaa gcacatagtg ccttatatga cacaaactta
    13021 atgatgcaat gcttctttaa ggcacgtaat aagtacggat tttttaaatt accctctgaa
    13081 attgtgtaaa acaaaagcct actttaaaaa gtttaaagta ggctttcttt taaagaacag
    13141 tagcctttca atcatttcct gcctgtattt aatacttttc cgcctgatag gtttagtcaa
    13201 aatgcagcca tcgaaacgca aatgtaacca aacagaagga gacttacatg agttcggttg
    13261 aaaatgtaat gacaaatgat gatctggacg agctgacagc catgttgcaa tcacttgatg
    13321 aaccagtaaa aaaagctgca caggttgaaa atactgatga tattgacgat ctgctgctcg
    13381 gcctagatgc tggcgtagcc atgagttctg atgatgttgc cgaagaactg ttcaatgaag
    13441 aaaaagcagg tgatttcagc tctgctttaa atgagttgga gttagcgcat gagcctataa
    13501 acgtaattaa cgctgaaagt gttgaagctg ccgaaaacga gccagaacaa ttgggattta
    13561 ttgaggttga agagtgtgtt gaggttaatg atgaattaaa agttcaacag tcaaatgata
    13621 gcaatacaaa taaaaaagct cgtactgcaa gaggtcctcg ttttacttta agtgataaag
    13681 atgattcgtt tttcaataaa gcgggcttag aaaaagatat tttcttagac gcttacgata
    13741 acgcgcctgt caaagcaaag gataagatat taaaccttct taattggttt agcggaggtc
    13801 cagatattag tgtttacacg gtaatttcca tgagacacct tctcacagaa aagaaggcta
    13861 caagtaatag tattaagatt gctttaatga gcaatccaga aaaaccgtat ccgcttaata
    13921 ctgcgtcaac tcaggctggg caaatgatgg ctgtatttcc agcgacagga attgccgtta
    13981 gagacggtgg aaatctaaca ttgaacgaag aatcaccgat cgttaagaag tttgtcgcgg
    14041 agtacactat tggatgacgt tcccctactt aaaataaagc ccatagagag ctttatagta
    14101 ctgggtaagc caatcacata cccagcacca caaaaacgcg ccagagagct tctcgtttgc
    14161 atttctggcg cgttttattt gattgccaga cataaaatca aatgcaaaaa taggtatata
    14221 cttacctatc gagaaagaag atgattgcag ccgaaaaaat caaacagcga aagcgcgaca
    14281 actctcttcg tgacctctgg agaacacctg actggctgtt ttctgccatt caacgttatc
    14341 ttggagtgac atttgatgtt gacgttgcct gcaacaagga caatgcaaag ctgcctaatt
    14401 tcataggcgt tgagcgtgat gctttgaaat ctgaatgggg acagccaggt acaattgcct
    14461 tcctcaatcc accctactcc aaaatctccc cctggattga tgcggctata cgtgagcagg
    14521 ctcgcggagt tacaacagtg atgctaattc ctcaatccct agatacaaag tggtatgagc
    14581 gtgcaacaga gtgtgcgaat gagacgatta ttctgtctgg tggccgcgta gcgtttgtcg
    14641 agcctgacgt aaatctgggt caggtagaag taaacatcaa cccaggtggc agtatgctcg
    14701 ttgtttttcg aggattctgt caggacgctg ggcactctat aagcaagatc cctttggacg
    14761 tcatgaaaag tctgggaggg tatgatcctg cgaatgtgat caggaaaaaa agaccatcaa
    14821 agaaggctgc ttagtttgtt ctggtgtctg taat
//
LOCUS       DAYPHN010000121        14676 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000121 DAYPHN010000000
VERSION     DAYPHN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.121"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..505
                     /locus_tag="ACG0BW_003432"
     CDS             <1..505
                     /locus_tag="ACG0BW_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFK7260357.1"
                     /translation="VFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQ
                     NASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRL
                     RGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQ
                     QNSFKHT"
     gene            661..2196
                     /gene="gadC"
                     /locus_tag="ACG0BW_003433"
     CDS             661..2196
                     /gene="gadC"
                     /locus_tag="ACG0BW_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFK7260358.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSKNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2327..3646
                     /locus_tag="ACG0BW_003434"
     CDS             2327..3646
                     /locus_tag="ACG0BW_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFK7260359.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNTTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLAQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4022..5404
                     /gene="dosC"
                     /locus_tag="ACG0BW_003435"
     CDS             4022..5404
                     /gene="dosC"
                     /locus_tag="ACG0BW_003435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFK7260360.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASLLSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKISQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5429..7828
                     /gene="dosP"
                     /locus_tag="ACG0BW_003436"
                     /gene_synonym="pdeO"
     CDS             5429..7828
                     /gene="dosP"
                     /locus_tag="ACG0BW_003436"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFK7260361.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRIRALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWISSVLPLK
                     I"
     gene            8086..8667
                     /gene="ddpX"
                     /locus_tag="ACG0BW_003437"
     CDS             8086..8667
                     /gene="ddpX"
                     /locus_tag="ACG0BW_003437"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFK7260362.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAEFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHAS"
     gene            8681..10231
                     /locus_tag="ACG0BW_003438"
     CDS             8681..10231
                     /locus_tag="ACG0BW_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFK7260363.1"
                     /translation="MKRSISFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVRFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10233..11255
                     /locus_tag="ACG0BW_003439"
     CDS             10233..11255
                     /locus_tag="ACG0BW_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7260364.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQRLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11252..12148
                     /gene="ddpC"
                     /locus_tag="ACG0BW_003440"
     CDS             11252..12148
                     /gene="ddpC"
                     /locus_tag="ACG0BW_003440"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFK7260365.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12145..13131
                     /locus_tag="ACG0BW_003441"
     CDS             12145..13131
                     /locus_tag="ACG0BW_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7260366.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDALNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPKSRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTAYGDNNHRCACWYPQQEVISV"
     gene            13124..14050
                     /locus_tag="ACG0BW_003442"
     CDS             13124..14050
                     /locus_tag="ACG0BW_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFK7260367.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRSET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSRSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14106..14537)
                     /gene="osmC"
                     /locus_tag="ACG0BW_003443"
     CDS             complement(14106..14537)
                     /gene="osmC"
                     /locus_tag="ACG0BW_003443"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFK7260368.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3592 a         3640 c         3747 g         3697 t
ORIGIN      
        1 ggtgttcaac gttgactacc tcggtggtca gattgggact ttcgccatca acttctcccg
       61 cccggcgggt caggtaattg cacagtacta tgaattcctg cgcctcggtc gtgaaggcta
      121 taccaaagta cagaacgcct cttaccaggt tgccgcttat ctggcggatg aaatcgccaa
      181 actggggccg tatgagttca tctgtaccgg tcgcccggac gaaggcatcc cggcggtttg
      241 cttcaaactg aaagagggtg aagatccggg atacaccctg tacgacctct ctgaacgtct
      301 gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca ccgacatcgt
      361 ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga
      421 agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca
      481 gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc gaaacgaacc
      541 cgtttcggga caatttccaa agtctgttca ctggcattag caacggaaaa tattgttctg
      601 aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta gggggataat
      661 atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg attctttgcc
      721 ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac atcgggcttt
      781 tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt gggactttgt
      841 gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca
      901 aatactctgg ggccgaggtg gggatttgca gcgatctcat ttggctatct gcaaatcgcc
      961 attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat cctgaaatgg
     1021 ccagcgctga atgaagaccc tattaccaaa actattgcag cactcatcat tctttgggcg
     1081 ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa agttggcttc
     1141 ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat ttatctgcac
     1201 tccggtgccc ccgttgctat cgaaatggat gcgaagacct tcttccctga cttctctaaa
     1261 gtgggcaccc tggttgtatt tgttgccttc attttgagtt atatgggcgt tgaagcttcc
     1321 gcaacccacg tcaatgaaat gagcaatcca gggcgcgact atccactggc tatgttactg
     1381 ctgatggtgg cggcaatctg cttaagctct gttggcggtt tgtctattgc gatggtcatt
     1441 ccgggtaatg aaatcaacct ctccgcaggt gtaatgcaaa cctttaccgt tctgatgtcc
     1501 catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct gttgctgggt
     1561 gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta tgtcacagcg
     1621 cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt accggtaacg
     1681 ctggtcattt cgcagctggt gattacttct atcgcgttga tcatcctcac caataccggt
     1741 ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat ttatctgtgt
     1801 gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc tgacttaaaa
     1861 cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggctgt tgtcggtctg
     1921 ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat ccagggtgac
     1981 tctaccgata tgtatgttga attactggtc gttagtttcc tggtggtact tgccctgccc
     2041 tttattctct atgctgttca tgatcgtaaa ggcaaagcga atactggcgt cactctggag
     2101 ccaatcaaca gtaagaacgc accaaaaggt cacttcttcc tgcacccgcg tgcacgttca
     2161 ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg ttataagata
     2221 agggagcgat aattcatcgc tccctttttc gtgcttgctg tcgtattgac ttaaccggga
     2281 taagtacgag aatgagcgca catctgttta ccggaaacca gcacatatgg atatctgctc
     2341 ccgaaacgag aaattagcga ttagaagacc agcgatactg gttgcactgg cacttttact
     2401 gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcgg gttcaaagcc
     2461 accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg ccacggtgtc
     2521 tcgcctcgac tggccaccgg tttcctcggt taacattagt aacaccacca gccgggcccg
     2581 tgtacaacaa caggcgatga tcgacaaact ggatcatctg caacgtctcg gcataaacac
     2641 ggtctttttc caggtcaagc cagacggtac cgccctgtgg ccatcgaaaa ttttgccgtg
     2701 gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc tgcaattcat
     2761 gctcgacgaa gcccacaagc gtgggatgaa agtacacgca tggtttaacc cctatcgcgt
     2821 atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgg ctcaacaacc
     2881 agcgagcgtc tatgtgcaac accgcgactg gatcagaacg tccggcgatc gctttgtcct
     2941 cgacccgggc atccctgaag ttcaggactg gatcacatca atagtcgcgg aagtggtttc
     3001 ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgagt caccgggttc
     3061 acggctaaat gataacgaaa cgtaccgcaa atacggaggc gcatttgcgt caaaagcaga
     3121 ctggcggcgc aacaatactc agcagttaat tgcgaaggta tcgcacacca ttaaaagcat
     3181 taagccggaa gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga
     3241 tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg ctgacacccg
     3301 tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact ggccgttctc
     3361 acggagtgcc gcacgttatg acgttttggc aaaatggtgg gcggatgtcg ttaaaccgac
     3421 caggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt caaagataga
     3481 gccagactgg atgattaacg gcggcgtgcc ggaactgaaa aagcagctcg atcttaacga
     3541 tgccgtgcca gaaattagcg gcacaatctt gttccgtgag gactatctga ataagccaca
     3601 gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaactc ccattcttac
     3661 tttgtaacaa gccggtgctt tccccccggc ttgttaccct cttgaaatat cccactagta
     3721 atcatgctta cttaagtcaa attaactacg caattaacca cacttagtta aacagtcata
     3781 aatcccctag taaaaatgta tgaatatgaa aaaacatacg atattcgctg tcaccgaaaa
     3841 ataattacga tgggctatac tcttctgaag aacagtagcg tagattgaaa ctgtcctaag
     3901 tcgcttattt gttttcagta tatcttcata tttcaggaga atatggtgca gttagtcgac
     3961 ctcgcacgtt gcgtttcgtt ttgattccgt ctgctttccc agccttataa gggtgtgaat
     4021 tatggagatg tattttaaaa gaatgaaaga tgagtggacc gggcttgtcg aacaagcaga
     4081 tccgctcatt cgtgctaaag ccgcggaaat tgccgttgcg catgcccatt atctgagtat
     4141 tgagttttac cgaattgtcc gtatcgaccc gcatgccgaa gaattcttga gtaatgaaca
     4201 agttgagcgg cagttgaaaa gtgcgatgga acgctggatt attaacgtgc tttctgccca
     4261 ggttgacgat gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag tgcatgcccg
     4321 cataggaatt ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa aaatcctcta
     4381 tccagtcatc ttctcttcgg attattccgc cgcagaaaaa cttcaggtct accatttctc
     4441 tattaacagt attgatatcg ctatggaagt gatgacccgc gcgtttacct ttagtgacag
     4501 tagtgcctca aaggaagatg aaaactatcg tatcttctcg ttactggaaa atgccgaaga
     4561 agaaaaagag cggcaaatag cctcattact ttcatgggaa atagatatta tctataaagt
     4621 cctgctggat tctgatttag gcagtagttt gcctttaagc caggctgatt ttggcctgtg
     4681 gtttaaccat aaaggtcgac attattttag cggcatagcc gaagtgggcc atatctcccg
     4741 tctgattcag gatttcgacg gtattttcaa tcaaaccatg cgtaacacca ggaatttgaa
     4801 taacagaagt ttacgggtaa aatttttatt acagataaga aataccgtat cgcaaattat
     4861 taccttgctg cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta tggatgtact
     4921 gacgaaatta cttaaccgcc gtttcctgcc gacgatcttc aaacgcgaaa ttgcccatgc
     4981 caaccggacc ggtacaccgc tgtcagtgct gattattgac gttgataaat tcaaagagat
     5041 taacgatacg tggggtcata acactggcga tgaaattctg cgtaaaatct ctcaggcctt
     5101 ttatgacaac gtccgcagta gtgattatgt tttccgctac gggggcgatg aatttatcat
     5161 tgttttgact gaagcttcgg aaaacgaaac gttacgtacc gcagaacgta ttcgcagtcg
     5221 ggtggagaaa accaaactga aagccgcaaa cggcgaagat attgccctct cactttccat
     5281 cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa tagccgatga
     5341 agctctgtat atcgccaaaa gacgaggtag aaaccgtgtt gaactctgga aagccagtct
     5401 ttagatgcgc caggatgcag aggtaatcat gaagctaacc gatgcggata atgccgccga
     5461 tggcattttt ttccccgccc ttgagcaaaa tatgatgggc gcggtgttaa ttaacgaaaa
     5521 tgatgaagtg atgtttttca accccgccgc agagaagctc tgggggtaca aacgtgaaga
     5581 agttattggc aataacattg atatgctgat tccgcgggat ttgcgtcccg cgcatcctga
     5641 atacatccgt cacaaccgtg aaggcggtaa agcgcgcgtc gaggggatga gtcgggagct
     5701 gcagctggag aaaaaagacg gcagtaaaat ctggacccgt tttgcgctat cgaaagtgag
     5761 cgccgagggg aaagtttatt acctggcgct ggtacgggat gccagcgtag aaatggcgca
     5821 aaaagaacag acccgacaat tgattattgc cgttgaccat ctcgaccgac cagtgattgt
     5881 cctcgatccg gaacgccata ttgtgcagtg caatcgtgca tttaccgaaa tgtttggtta
     5941 ctgcattagc gaagccagcg gtatgcagcc cgatacactc ctgaacattc cagaattccc
     6001 tgctgataac cgcattcgtt tacaacagtt gctatggaaa accgcccgcg atcaggacga
     6061 atttctgctg ttgacgcgca ccggtgaaaa aatctggatt aaagcctcta tcagcccggt
     6121 ctatgatgtg ctcgcgcatc tgcagaacct ggtaatgact ttctcggata tcaccgaaga
     6181 acggcaaatc cgccagcttg aaggcaatat tctcgccgcc atgtgcagca gcccgccatt
     6241 tcatgaaatg ggggaaatta tttgtcgtaa catcgaatct gtactcaacg aatcgcatgt
     6301 ttcgctgttc gcgctgcgca acgggatgcc gatacactgg gcgtcatctt cgcacggtgc
     6361 agaagttcaa aatgcgcaaa gctggtcagc gaccattcgt cagcgtgatg gtgcgcctgc
     6421 ggggatcctg caaattaaaa cctcgtcagg agcagaaacc agcgccttta tcgaacgcgt
     6481 ggcagatatc agccagcata tggccgcgct ggcgctggaa caggaaaaaa gccgtcagca
     6541 tattgaacaa ctcatccaat ttgatccgat gaccggtctg ccaaatcgca ataacctgca
     6601 caattacctc gatgacctgg tcgacaaagc cgtctctccg gtggtgtatc tcatcggtgt
     6661 tgaccatatt caggatgtga ttgatagcct tggctatgcg tgggccgatc aggcattgct
     6721 ggaagtggtc aatcgctttc gtgaaaaact caaaccggat cagtatctct gtcgtatcga
     6781 aggtacgcag tttgtcctcg tgagcctgga aaacgacgtc agtaacataa cccaaatcgc
     6841 cgatgagcta cggaatgtgg tcagcaagcc gataatgatt gacgataaac cattcccgct
     6901 caccttgagc attggcatca gctacgacgt gggtaaaaac cgcgattacc tgctctccac
     6961 tgctcacaat gcaatggatt atattcgcaa gaatggcggt aacggctggc agttcttcag
     7021 cccggcgatg aacgaaatgg taaaagagcg tttggtttta ggcgcggcgc tgaaagaagc
     7081 gattagcaat aaccaactaa aactggttta ccagccgcaa atcttcgcag aaacgggtga
     7141 gctctacggc atcgaagccc ttgctcgctg gcacgatccc ctgcatggtc atgtgccccc
     7201 ttcacggttt attcctctcg cagaagagat cggtgaaatc gaaaatattg gacgctgggt
     7261 catcgcggaa gcttgtcgtc agttagcaga atggcgtagc cagaatattc atatcccggc
     7321 gttatccgtt aacttgtcgg cgttgcactt tcgcagtaat cagctgccta atcaggtgtc
     7381 tgatgcaatg caagcctggg gtattgacgg ccaccagctg acggtggaaa tcacggaaag
     7441 catgatgatg gaacacgata ccgaaatctt taagcgcatt cagatcctgc gcgatatggg
     7501 cgtaggttta tctgtcgatg atttcggcac gggtttttct ggtttatccc gcttagtcag
     7561 tcttccggta acggaaatca aaattgacaa aagctttgtc gatcgttgtc tgaccgaaaa
     7621 acgcatccga gccttacttg aagccattac cagcattggg caaagcctca atttaaccgt
     7681 cgtggcagaa ggcgtcgaaa ccaaagaaca atttgagatg ctacgcaaga ttcactgtcg
     7741 cgttattcag ggatatttct tttcccgccc cctacccgcc gaagaaattc ctggctggat
     7801 tagcagcgtg ttaccgctga aaatctgaca attttctccc gcccgcactc gcgggttttc
     7861 atttaacgtg acactgtcac ttaaatactg tgatttcagc tacgattccg ggagattcct
     7921 tccttaactt cccctcttgc accaaaactg cactacgctg cacaaatgtt gatcaaaagt
     7981 taaaaaatat aactttaagc cagcattctg gcgcttatcc cggcgtggca tgagatctgc
     8041 ataagcggaa agcgcagcaa tttttgtctt atacaggaac cgtttatgtc agataccacc
     8101 gaactggttg atttagccgt gatcttccct gatctggaga tcgaattgaa atacgcctgc
     8161 gctgataaca tcacaggtaa agctatttat cagcaagcgc gttgtctgtt acacaaggat
     8221 gcgattaccg cgctggcgaa aagtatcagt atcgcccagc tgtcaggctt acaactggtg
     8281 atttacgatg cgtatcgccc acaacaagca caggcgatgt tgtggcaagc ctgcccagac
     8341 ccgcaatatg ttgttgatgt gacggtcggt tctaatcaca gccgtggcac ggcgatcgac
     8401 ctgacgcttc gtgatgagca cgggaacata ctggatatgg gtgcagagtt tgatgaaatg
     8461 cacgaacgtt cccatgctta tcacccttcc gtcccgcccg ccgctcagcg caatcggctg
     8521 ttgctgaatg cgattatgac tggcggtggc tttgtcggta tcccgagcga atggtggcac
     8581 ttcgaattac cccaggcagc gagttaccca ctactcgctg atcaattcac ctgttttata
     8641 tcccctggca cacagcacgc cagttaatca ggaaactgtc atgaagagat cgatttcgtt
     8701 tcgtcctaca ttgctcgcga tcgtccttgc cacaacaatg ccggttgcgc acgccgccgt
     8761 accgaaagat atgctggtga tcggtaaagc tgccgaccca caaaccctcg acccagcggt
     8821 gacaattgat aataacgact ggacagtgac ctacccgtct tatcagcgac tggttcagta
     8881 caaaacggac ggtgataaag gctcaaccga cgttgaaggc gatctggcga gtagctggaa
     8941 agcgtctgac gatcaaaaag agtggacatt caccctgaag gataatgcta aatttgccga
     9001 tggcactcct gtcactgccg aagcagtaaa actctctttt gagcggttac taaaaatcgg
     9061 ccaggggcca gcagaagcat ttcccaaaga tttaaagatt gatgctcccg acgaacatac
     9121 agtgaggttt acccttagcc agccattcgc accgttcctc tacacgctgg cgaatgacgg
     9181 tgcatccatt atcaatccgg cggtcttaaa ggagcatgcg gcggatgatg cccgtggttt
     9241 cctcgcgcaa aataccgctg gctccggacc gtttatgctg aaaagctggc aaaaaggtca
     9301 gcaattagtt ctggtgccaa atccgcatta ccccggcaat aaaccgaatt tcaaacgggt
     9361 atcggtaaaa attatcggtg aaagtgcttc ccgtcgcctg cagctctccc gtggcgacat
     9421 tgacattgcc gatgcgctgc cggtggatca actcaacgca ctgaagcagg aaaacaaagt
     9481 caatgtggca gagtatccgt cactgcgcgt cacctatctg tatctgaata acagcaaagc
     9541 gccacttaat caggcggatc tgcgtcgggc catttcctgg tctaccgatt atcagggtat
     9601 ggttaacggc attctgagtg gtaacggaaa acaaatgcgc ggcccgattc cggaaggcat
     9661 gtggggatac gatgcgacgg caatgcaata caaccatgac gaaacgaaag ccaaagccga
     9721 atgggataaa gtgacgagca aacccaccag cctgacgttt ctctattctg ataatgatcc
     9781 gaactgggag cctattgctc tggcgacaca atccagtctc aacaagctgg gcatcaacgt
     9841 gaagctggaa aagctggcga acgccaccat gcgcgacaga gtgggaaaag gtgattatga
     9901 cattgcgatt ggcaactgga gtccggattt tgccgacccg tatatgttta tgaattactg
     9961 gtttgagtca gacaaaaaag gtctgccggg taaccgctcg ttctatgaaa acagtgaggt
    10021 cgataagtta ctgcgcaatg cgctagcgac caccgaccag acgcagcgta ctcgggacta
    10081 ccagcaggca cagaaaatcg tcattgatga cgctgcttat gtgtacctgt tccagaaaaa
    10141 ctaccaactg gcgatgaaca aagaggtgaa aggttttgtg ttcaatccca tgctggaaca
    10201 ggtcttcaat atcaatacca tgagtaaata acgtgacctt ctggagtatt ttacgccaac
    10261 gctgctgggg gctggtgctc gtggtggcgg gcgtctgtgt gataacgttt attatctcgc
    10321 acctgatccc cggcgatccg gcgcggttac tggcgggtga ccgcgccagc gatgctatcg
    10381 tggataatat tcgccagcaa ctgggactgg accagccact gtacgtacag ttttaccgct
    10441 acgtcagcga cctgtttcag ggtgacctgg gaacatccat tcgtaccggg cgtccggtgc
    10501 tggaagagtt gcgtatattt ttcccggcga cgctggaact ggctttttgt gccctgctgc
    10561 tggcactcct gattggcatc ccgctgggca tactctctgc agtctggcga aatcgctggc
    10621 tggatcatct ggtgcgaata atggccatta ccggaatctc cacacctgcg ttctggcttg
    10681 ggctgggcgt cattgtgctg ttttatggtc atctgcaaat tcttcccggc ggcggaaggc
    10741 ttgatgactg gctggatcca ccaacgcacg ttaccggctt ttatctgctc gatgcgctgc
    10801 ttgaaggcaa cggtgaagtc ttcttcaatg cgctgcaacg tctcatctta ccggcattaa
    10861 cgctggcgtt cgttcacctg ggaattgtcg ctcgccagat ccgctcagct atgctggaac
    10921 aattgagtga agattacatt cgtaccgccc gggccagcgg cttgcccggc tggtatatcg
    10981 ttttatgtta tgcgctaccc aatgcgttga tcccttcgat taccgtattg ggtctggcgc
    11041 tgggcgattt gttgtatggc gcagtgctca ccgaaaccgt ttttgcctgg cccggaatgg
    11101 gggcatgggt agtgacatca atacaggcgc tcgacttccc tgcggtgatg ggatttgccg
    11161 tcgtggtttc gtttgcttat gtgctggtca acctggtggt ggatttgctc tatttgtgga
    11221 ttgatccgcg tatcggacgt ggaggtggtg aatgatgcta agcgaggaaa cgtcagccgt
    11281 gcgcccacca aaacaaacgc gatttaacgg tgcaaaactg gtatggatgc tgaaaggcag
    11341 cccgcttacc gtgaccggcg cagtcatcat tgtattaatg ctactgatga tgattttttc
    11401 accgtggctg gcgacgcatg atcccaacgc cattgattta accgcccgcc ttttgccgcc
    11461 ttctgcggcg cactggtttg gcaccgatga agtgggacgc gatctgttta gccgcgtact
    11521 ggtcggcagt cagcaatcaa ttctcgccgg attagtggtg gtcgccattg caggtatgat
    11581 ggggtcgcta ctcgggtgtc tatccggtgt gcttggcgga cgcgcagacg cgattatcat
    11641 gcgcatcatg gacattatgc tgtcgattcc ttcgctggtg ctgacaatgg cactggcagc
    11701 cgctctcggg ccgagtttgt ttaacgccat gctggcgatt gctattgtgc gaattccctt
    11761 ttatgtgcgc ctggcgcggg ggcaaacatt agttgttcgc cagtatacct atgttcaggc
    11821 ggcgaaaacc tttggtgcgt cgcgttggca tctgatcaac tggcatattt tacgtaactc
    11881 cctgccgccg ctgattgtgc aggcatcgct ggatatcggt agcgcgattt taatggccgc
    11941 cacgttggga tttattggcc tgggtgctca acaaccaagt gctgaatggg gggcgatggt
    12001 ggcgaatggt cgcaactatg tgctcgatca atggtggtat tgcgcatttc cgggggcagc
    12061 gattttgctt accgccgtcg ggtttaatct ctttggtgat ggtattcgcg atctgcttga
    12121 cccgaaagca ggaggaaagc agtcatgacc caacccgttc tggacattca acaactgcat
    12181 ttgagtttcc ccggttttaa cggtgatgtt cacgcgctca acaatgtgtc attgaaaatc
    12241 aaccgcggtg aaattgtcgg tctggtggga gaatccggct cgggtaaatc agtcactgca
    12301 atgttgatta tgcgtctgtt accgacgggc agttattgcg tacatcgggg acatatttca
    12361 ctgctgggag aagatgctct taacgctcgg gaaaaacagc ttcgtcagtg gcgcggcgca
    12421 cgagtggcga tgatctttca ggaaccgatg accgccctta atccgacacg tcgaataggt
    12481 cttcagatga tggacgtgat ccgccatcat caaccaaaaa gtcgtcggga agccagagct
    12541 aaagcgattg ccctgctgga agagatgcaa atcccggatg ccgtggaagt tatgtcgcgc
    12601 tatccgtttg agctttcagg tggtatgcga cagcgggtaa tgattgcgct ggcattctcc
    12661 tgcgagccgc aattgattat tgccgacgaa ccgactacgg cgctggacgt cacggtacag
    12721 ttgcaggtac tgcgtctgct taaacataaa gcccgcgcca gtggaactgc ggtactgttc
    12781 atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata gcgtttacgt gatgtatgcc
    12841 ggaagcgtga ttgagagcgg cgtgacggca gacgttatcc atcatccccg gcatccgtat
    12901 accattggtt tgctgcaatg cgcaccggaa catggaatac cgcgccagcc attacccgct
    12961 attccgggga cggtaccaaa cctcacccat ttgcctgacg gctgcgcttt tcgcgatcgt
    13021 tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc taacagcgta tggtgacaac
    13081 aaccaccgct gcgcctgttg gtatcctcag caggaggtca ttagtgtctg acacgttatt
    13141 aacgttacgc gacgtccata tcaatttccc ggcccgaaaa aactggcttg gtaaaactac
    13201 ggaacatgtt catgccatca atggtattga tttacagatc cgtcgtagtg aaaccttagg
    13261 gatcgtcggt gagtcaggct gcggcaaaag caccctcgca cagcttttaa tgggtatgct
    13321 gcaaccgagc cacgggcagt acatccgttc aagatcacaa cgcattatgc agatggtgtt
    13381 tcaggacccg ctctcttcgc tcaatccgcg cttaccggtg tggcgcatca tcacagaacc
    13441 gctctggata gctaagcgta gtagtgaaca acagcggcga gcgttggcag aggagctggc
    13501 tgtgcaggtg ggtattcgtc cggagtatct cgatcgcttg cctcatgcgt tctcaggcgg
    13561 gcagcggcaa cgtatcgcca ttgccagagc actctcttcg cagcctgacg tgattgtgct
    13621 tgatgaacca acctcggcgc tggatatctc cgtgcaggcg cagatcctca atttactggt
    13681 aacgctacag gaaaatcgcg ggctgactta tgtgctgatt tcacacaatg tctcggtgat
    13741 acgtcatatg agcgatcggg tggcggtgat gtatctcgga cagattgtgg aactaggaga
    13801 cgcgcaacag gtgctgacag ctccggcaca tccatacacc cgattattgc tggattccct
    13861 ccccgccatt gataaaccgc tggaggaaga atgggcatta cgtaaaacgg atctaccagg
    13921 aaaccgcacg ttgccacaag gctgtttttt ccgcgaacgc tgccctctgg caacccacgg
    13981 atgtgaagtc cggcaatcat taacaataag ggaggacgga cgtgagatcc ggtgctggcg
    14041 ggcgctgtag agacgctaac aacgcatcag gcaatcacgc gcctgacgcg tcatccggca
    14101 acgctttacg atttcaactg gtaatcaagc gtaatttcag ctttcagtac ctgagaaacc
    14161 gggcatcctg cttttgcttt ctggataatg ccgtcaaaag tagaggcatc aatacccggc
    14221 accgcaactt cgctcttcag tgcgatttta gtaatcgcaa aacctgcatc cactttatcc
    14281 agcgacacat cggcggtggt atcaatcgat gttggcgtga atcccgcttc ccccagcatt
    14341 aatgaaagcg ccattgagaa acatgcggca tgcgctgcgc caatcagttc ttcagggttg
    14401 gttccttttt cgccttcaaa acgcgtgtta aatccatacg gctgttggtt cagcacgccg
    14461 ctctcggtgg atacagttcc cttcccgcgt ttgatatcgc cttcccagtg tgcctgacct
    14521 ttcttatgga ttgtcattgt tgctctcctg tgggcttaaa aaggaaatta tagactacgt
    14581 gagaaaccgc acgaggataa gcaggatatt ccgaataaaa tccggcgaaa acattatctc
    14641 tgtacttaaa ttaaattccc acaatagata acgggg
//
LOCUS       DAYPHN010000122        14527 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000122 DAYPHN010000000
VERSION     DAYPHN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.122"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..704)
                     /locus_tag="ACG0BW_003444"
     CDS             complement(<1..704)
                     /locus_tag="ACG0BW_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFK7260369.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLARE"
     gene            complement(882..1793)
                     /gene="gsiD"
                     /locus_tag="ACG0BW_003445"
     CDS             complement(882..1793)
                     /gene="gsiD"
                     /locus_tag="ACG0BW_003445"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFK7260370.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1796..2716)
                     /gene="gsiC"
                     /locus_tag="ACG0BW_003446"
     CDS             complement(1796..2716)
                     /gene="gsiC"
                     /locus_tag="ACG0BW_003446"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFK7260371.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2734..4272)
                     /gene="gsiB"
                     /locus_tag="ACG0BW_003447"
     CDS             complement(2734..4272)
                     /gene="gsiB"
                     /locus_tag="ACG0BW_003447"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFK7260372.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4292..6163)
                     /gene="gsiA"
                     /locus_tag="ACG0BW_003448"
     CDS             complement(4292..6163)
                     /gene="gsiA"
                     /locus_tag="ACG0BW_003448"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFK7260373.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQCGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEHIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPKHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6150..7115)
                     /gene="iaaA"
                     /locus_tag="ACG0BW_003449"
     CDS             complement(6150..7115)
                     /gene="iaaA"
                     /locus_tag="ACG0BW_003449"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFK7260374.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     EMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNIALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7319..8554
                     /gene="moeA"
                     /locus_tag="ACG0BW_003450"
     CDS             7319..8554
                     /gene="moeA"
                     /locus_tag="ACG0BW_003450"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFK7260375.1"
                     /translation="MEFTTGLMSLDIALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAARLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDAGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VEAGEWVEVEPFNALFGGL"
     gene            8554..9303
                     /gene="moeB"
                     /locus_tag="ACG0BW_003451"
     CDS             8554..9303
                     /gene="moeB"
                     /locus_tag="ACG0BW_003451"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFK7260376.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVDSARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9379..10041)
                     /gene="fsa"
                     /locus_tag="ACG0BW_003452"
     CDS             complement(9379..10041)
                     /gene="fsa"
                     /locus_tag="ACG0BW_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFK7260377.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            10172..11071
                     /gene="ybiY"
                     /locus_tag="ACG0BW_003453"
     CDS             10172..11071
                     /gene="ybiY"
                     /locus_tag="ACG0BW_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFK7260378.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11077..13509
                     /gene="ybiW"
                     /locus_tag="ACG0BW_003454"
     CDS             11077..13509
                     /gene="ybiW"
                     /locus_tag="ACG0BW_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFK7260379.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13655..14470
                     /gene="ybiV"
                     /locus_tag="ACG0BW_003455"
     CDS             13655..14470
                     /gene="ybiV"
                     /locus_tag="ACG0BW_003455"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFK7260380.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
BASE COUNT         3548 a         4022 c         3630 g         3327 t
ORIGIN      
        1 ttttcacgcg cgagttgatt cttggcaatg atctcatggc cttgtttcaa aaatagaact
       61 tcatcattag cgctgatttg atcaagatac tcgttcagat atcgccagtt taaatcgaca
      121 tgaattttat ccaccgctaa caaatcgtga tctttgccgt tgatgtgtag cgtaaaagag
      181 atacattttt tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag
      241 tcatctgaag ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc
      301 cctgactcgt cgacaagcgg tccatttact tgttcctgat gataaactac gattttatca
      361 ttcgccctcc gcgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct
      421 aaaaatggca tcatttccat tttaagaatc acggcgcgtt gaatctcctc tgtatttttt
      481 aataaataca gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag
      541 ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgctccgat
      601 accagttgat agttaatgat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat
      661 aaaattatca gccttatctt tatcttttcg tataaactca gcatgttaat acatttaaca
      721 aaataactat ctgaacgctg tgccgctgca aaaattgtcc cttgctgcgg ctttttaacc
      781 aaattacccg attctacaaa atcagcagaa agttacaaca cttgcttagg gcaataattt
      841 cctgtgaggt gattaccctt tcaagcaata ttcaaacgta actatccttt aattttcgga
      901 tccagcgcat cgcgtaaacc atcacccaac aaattgaacg ccagtacagt cagaaaaata
      961 gccagggccg gaaaaatagc gacatgcggc gcgataacca tatccgctcg tgcctcattg
     1021 agcattgctc cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg
     1081 gcggcagaga taatcgaggt accgatgcgc atggtgaaaa acaccacgat agacgagaca
     1141 gtcccgggca agatatggcg caataagatg gtcatgtcgc tggcaccaat actgcgcgcc
     1201 gattcaataa aagtttgctg tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac
     1261 gcggggatgg aaaaaatggc gactgcaata atcacgttag caatgccgct tcccaacacc
     1321 gcaacaacag cgatcgccag taaaatacca gggaaggcaa acagcacatc gcaaatgcgc
     1381 atgatcagcc gatcccacca gccttcataa tatccagcca acaagcccag caacgtcccg
     1441 atcgccgcac cgataaacac ggcaaacacg cccgccgcca gcgagatttg tgcaccaacc
     1501 aggacacggc tgaaaatgtc acgccccagt gaatcaacgc caaaccagtg ctgcaaagaa
     1561 ggtccgttat tcagattgtc ataatcaaaa taattttcgg cgtcataggg agcgatccag
     1621 cgtgcaaaaa tagccaccac aatcaataaa ataacgaaca atgcggcggt catcgccata
     1681 tgctgacggc gaaatcgtcg ccagaattca tgccacggtg tacgtacctg gtcaggtttg
     1741 accagtggca tggcgtttaa caccgcctga cgtcgccagt taaatagtcg catccttact
     1801 tgtacctgat agccgggtta atggcggcgt aaagcacatc caccactaag ttgataagaa
     1861 taaattccag cgagaaaagc agaatttccg cctgaatcac cgggtaatcg cgcatttcta
     1921 cggagtcaac gagtaagcgt ccaagtccgg gccagttgaa aaccttctcc accacgatgg
     1981 aaccaccgag caaaaagcca aactgtaagc ccatcatggt cacaaccggg atcatcgcgt
     2041 tacgtagccc gtgtttgagg acaacccagg tttcgctcac ccctttcgcc ctcgcggtac
     2101 gcatataatc ttcgcttaaa acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg
     2161 ctgccactgc cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc
     2221 ccacggtagg cagccagccc agttcaacgg agaatacctg aattaaaagc atccccagag
     2281 caaatgccgg aaacgagatc cccgacaccg caatggtcat actcaatcga tccggccaac
     2341 gattacgcca gacggcggcg ataattcccg ccgccatacc aaatatcacc gcccagacca
     2401 tactggttat ggtcagccac agcgttggca taaagcggct ggcaatctca tcggcaaccg
     2461 gacgacgcga caccatcgac aggccaaaat ccccctgcac agcattgctg atatagtgcc
     2521 agaactggtg atacagcggc tgatccaacc ccagctgctg acgcaccagt tctataacct
     2581 gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc atatgcacaa
     2641 ataaaaacac cagcaccgag acgataaaca gcgttggaat caaccccagt aagcgtttga
     2701 taacgtaatt aagcattcca ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct
     2761 gaaaccggtg tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag
     2821 tttttctacc accagcggga tccacggcga ctcttgccag atgatatcct gcgccgcctt
     2881 atataaacgg gtcttttctg ccggatcatt agttttcagt gcctgagcca ggaagtcatc
     2941 cacctgttta ttgctgtaaa acgcggtatt aaacagcgtc ggtggccagt tctgcgaggc
     3001 aaacagcggc gatagtgccc agtccgcttc gccggttgaa gccgaccagc cagtgtagaa
     3061 catccgcacg ccgctctctt tttgcccttt accttcaact tctgccgccc gctgtccggc
     3121 atccatcgca gtcacctggg ctttaatccc gacctgcgct aactgctgct gggtaaattg
     3181 cagcactttc tgcgcggtgc tgtggttatg tgacgaccac agcgtggtat tgaaaccgtt
     3241 gggatatccc gcctctttca gtaattcgcg cgctttcact ggatcgtaag gccacggttt
     3301 atagctttgc gcgtaggcga tactcggcgg taccacacca gtagctggcg ttgcatagcc
     3361 cgcgaaggcc actttcacca gcgccgggcg gttaatggcg taattcagcg cctcacggac
     3421 cttcgggtta tcgaacggct tttgcgtcac gttcatactg atataacgct gcataattga
     3481 cggactggcc atcaactcga tatttttgtt tttctccagc agtgcggcct gctcgtaagg
     3541 aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc
     3601 cgccaccgga cgccaggtta tgctgtccag tttgggcaat cctggctgcc agtaacccgc
     3661 gaattttttc accttcacaa aatcggtctg attccaggta tccagttcat acggcccggt
     3721 tcccaccgga tgaaaaccaa tctccttgcc atatttttcc agcgctgccg gcgaaatcat
     3781 cgcggtcgcc ggatgggcaa gaatattaat aaacgctgag aacggctgtt tgagggtaat
     3841 ctttaccgtt gttggatcga tcgcttccgt tttggcaata ttcttataca ggttgtagcg
     3901 tttaagatga ttcgccggat cgctggcccg gtccagattc gcttttaccg ccgctgcgtt
     3961 gaagtcggtg ccatcctgga atttaattcc ttcccgcaat ttcacggtgt aagtaaggcc
     4021 gtcatcggaa acggtataac tctccgccag cacgtttttc agtttcatct ctttatcgag
     4081 accgaacagc ccctggtaaa acgatttcgc tacggcctga gataacgtgt catttgcgtc
     4141 atacggatcg agcgtggtga agttcgatcc taccgccacc accacatctt tggcagcgaa
     4201 tgcacaagat gccatcaacg ctgtcgcaat gcccagcgcc actaacccac tacggtgtac
     4261 agctcttgcc attttattct ccgcctgaat gttatctacg catgaatgcg tattctgatt
     4321 gtggttgtgc gacgtaatgc cccggcccga cgcattgcaa cgagacggct gccacctctt
     4381 cgccacgcag atgaatattg ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt
     4441 gtcgggacgg ttcagcgacc ggaactgccg ccagtaattt acgcgtataa ggatgcttcg
     4501 ggttttcaaa gaccgcgcgc cgtggaccaa tttcaacaat ttgtccgaga tacatcaccg
     4561 ccacacgatg actaatccgc tctaccacgg ccatatcgtg ggagataaac agatacgcaa
     4621 tgccgaaatc acgctggaga tcgagcaaca agttgataat ctgcccgcga atagaaacat
     4681 ccagcgccga gacggcttcg tcggcaatga tcacttttgg attcaatgcc aacgcgcgag
     4741 caatgcagat gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt
     4801 caggtaacag gcccacgcgc tccagcaacc acgcaacgcg tgcagccgct tctttacctg
     4861 gcaataaacc gtgtacgcgc agcggttcga taatcgaatc accgatggtc tgacgagggt
     4921 ccagcgaagc gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt
     4981 taccggatga caaggtatcg attcgctgac cgttaaagat aatttcgccg ccctgcgatt
     5041 cgaccaggcg cagcaacgcc cgcccggtag tggatttacc gctgccagac tcgcccacca
     5101 gcgatagcgt ttcgccaggc cagagatcaa aactgacttt ctcaacggca tgcacttccc
     5161 gcgttacgcg attcaacaaa ccgctgcgca aagggaaacg ggtgaccaga ttacgcaccc
     5221 gtaaaacagg ttcgccatcc accaccgttt tctgctcgat ggggggttcc tgtttcgctg
     5281 gatgttcaag cgatatcaat gggaaacgtc ggggataatc taaccctttc atcgcaccaa
     5341 gttgcggaac agcagctaac agcgcacggg tgtacggatg ttgcggtgca tgaaaaatat
     5401 gttcgacgct acccgtttcc accgcctcgc cctgatacat caccagtacc cgatcggcaa
     5461 tctctgccac cacgcccata tcgtgagtga taaagataac gcccatcgac atctcttttt
     5521 gcaatacttt gattaattgc aggatctgcg cctgaatagt gacatccagc gcggtggttg
     5581 gctcatcggc aatcagcacc gccgggcggc atgacagcgc catcgcaatc atcactcgct
     5641 ggcgcatccc gccagagagt tgatgcggat aacgtgaaag aatggtttgt gcctccggaa
     5701 tgcgtacctg atccagcatc cgcttcgcct cgaccattgc ttcttcacga ctggcgtttt
     5761 gatgcagacg aatagattca gcaatctgtt cacccacagt aaataccggg ttcagcgatg
     5821 tcatcggctc ctgaaatatc attgccatat ccgcaccgcg cacatggcgc atttgtgcag
     5881 cgctctgctc gctaagttca atcacttcgc gactgcgccg ccgcaacagc atttgatcgc
     5941 actgcactaa accgcccgcc tgttccaaca agcgcattaa tgccagcgca gtcactgact
     6001 taccagagcc ggattcgcca acaattgcca gcgtctcacc gcattgcagg ctaaaagaga
     6061 gattgcggac cgcagctatt ttctgctggt cctgcataaa ggcaatattc agattttcaa
     6121 ccgccagcac atcaccggca tcaagttcat cactgtgtgg caacggtgtc ccctttttca
     6181 cggtagatac cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata cattccttcg
     6241 gtgttaaacg gtagcgcgat attaccttca tggtcgatag cgattaagcc accgctaccg
     6301 ccaagcgcag ggagtttttc cattactacc cgctcgcagg cttccgcgag acttaatccg
     6361 ccgtaatcca ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa gacttcgccc
     6421 gtgccggtac atgaaaccgc cacactggcg ttattggcgt agcatccggc ccccactaag
     6481 ggactatcgc caactcgtcc gggtaattta ttggtcattc cgcctgtgga cgtggctgcc
     6541 gccagattgc cgtctaaatc caacgccacg gcccccacag tgcccatttt ctgtttttca
     6601 tccagtggcg caccgctatg gtcgaggacc atttcccctt cctcgcgcgc tgccagtagt
     6661 tgttcataac gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat gccatgagca
     6721 aacgcaaaat tttctgcccc ttcgccaatc atcatcacat gcgggctttg ctccatcacc
     6781 agccgggcgg caagaaccgg attacgcaga tgactaacgc ccgccaccgc accggctttc
     6841 agggtgttac catccatcac acaggcgtcc agttcatggg tttcatcacg cgtaaagaca
     6901 gcgccaattc cggcgttaaa cagtggacac tcttccagca gacgcaccgc ttccgtcacc
     6961 acatccagcg cactttcgcc cgcttccagc attttctgcc cggtttcaac aatggcagac
     7021 agcgcctcga tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct aattgcacct
     7081 gcgccaccat gaattgcaat gactgctttg cccatagtat tcgtccatta agggtggctc
     7141 gccacttttg ctataaatat cagaattttt atgaattact cattcaattt ttgaatatag
     7201 aaagatggat atggcatgta aaggcaggag ccagcgctac gcccgaggtg gcatacagtc
     7261 tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat
     7321 ggaatttacc accggattga tgtcgctcga catcgcgctt aatgagatgc tttctcgcgt
     7381 taccccactg accgcccagg aaacgctgcc actggtacag tgttttggtc gtattctggc
     7441 gagcgatgtc gtttcgccac tggatgtccc ggggtttgat aactccgcta tggacggcta
     7501 cgcggcgcgt ttagccgata ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt
     7561 tgccggtcag ccgtaccatg gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg
     7621 tgcgccggtg ccggaaggct gcgaagcggt ggtgatgcag gaacagaccg aacagactga
     7681 cgctggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga
     7741 agatatctct gcaggtgcgg ttgttttccc ggcgggaaca cgcctgacta ccgcagagct
     7801 gccagtaatt gcttcactgg ggattgccga agttccggtg attcgtaaag tgcgtgtagc
     7861 gctgttttct accggtgatg aactccagtt gccaggccag ccgctgggcg acggccaaat
     7921 ctacgatacc aaccgtctcg ccgtacacct gatgttagaa cagttgggat gcgaggtaat
     7981 caacttaggg attatccgcg acgatcccca tgccctgcgc gccgcattta ttgaagccga
     8041 cagccaggcg gatgtggtga tcagttccgg cggtgtttca gtgggtgaag cggattacac
     8101 caaaacgatt cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccagg
     8161 taaaccgttc gcgttcggta aactcagcaa tagctggttc tgcggcctgc cgggcaaccc
     8221 ggtttcagca acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg
     8281 caataccgcc agcggcctgc ccgcgcgcca gcgtgtacgc acggcatcgc gtctgaaaaa
     8341 aacgccagga cgccttgatt tccagcgcgg cgtgctgcaa cgcaacgccg atggcgaact
     8401 ggaagtgacg accaccggac atcagggttc acatatattt agctccttta gcctcggcaa
     8461 ctgctttatc gtgctggagc gcgaacgcgg caatgtggaa gcgggcgaat gggtggaagt
     8521 agaaccgttt aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaggagatg
     8581 ctgcgctata accggcaaat cattctgcgc ggctttgatt ttgatggtca ggaggcgcta
     8641 aaagattctc gtgtgctgat cgtcggcctc ggtggactcg gctgcgcagc ctcacagtat
     8701 ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgacacggt ttcgctctca
     8761 aatttacagc gccaaacgct gcacagcgat gccacagtcg ggcaaccgaa ggtggattct
     8821 gcccgtgacg ccctgacgcg gatcaaccca catatcgcga ttacgccagt caatgcactg
     8881 ctggatgacg cagaacttgc tgcgatgatt gcaaaacacg atctggtgct cgactgtacg
     8941 gataacgttg cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg
     9001 gtttccggcg cggcaattcg catggaaggt caaatcacgg tcttcactta tcaggacggc
     9061 gagccttgct accgctgcct cagccgtttg tttggcgaaa atgcattaac ctgcgtagaa
     9121 gcaggcgtga tggcaccgtt gatcggcgta attggttcgt tgcaggcaat ggaagcgatc
     9181 aaactgctgg caggttatgg caaacctgcc agtggcaaaa tcgtcatgta cgatgcgatg
     9241 acctgtcagt ttcgcgaaat gaaactgatg cgtaatccgg gatgtgaggt gtgcgggcag
     9301 taattgccac ctccaggcct gataagacgc ggcactgcgc cacgtcatca gcaatttatg
     9361 aggatgcaga acgtccggtt aaatcgacgt tctgccaaac gctccctgcc agtcctgctc
     9421 aaacttcgcc acagcggcat caaccgccgg atagctaatc atctgttgtg ccacatccag
     9481 tggcagagta attgattcac atcctgccag taagcagtcc agcgcctgac gcggggtttt
     9541 gaaacttgcg gctaacactt ttgcctgcgg cgcatgcatt ttcaataact ggtgtaagtc
     9601 ggtcacagtc tgaatgccgc taccgccctg agcatcaata cgattaacgt aaggcgcaac
     9661 atattccgca cctgccagcg ccgacagtag cccttgtgct gcgccatata ccgcggttcc
     9721 cagcgtcgga atcccttccg cttttaacat cttaattgct gccagcccct cagcggtcac
     9781 cggaactttc accacgatat ccgcaataat agagcgcagc ttacgcgcgt cattcaccat
     9841 cccttcagca gtggtagcca ttacctgggc aaacagacgc ccctgaccgc ccatcgcttc
     9901 atgaagttcc ggaagcacaa cctccagcgg ttttttaccc gctgcgataa tgcttgggtt
     9961 agtggttaca cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa caacgtctga
    10021 agtatccaga tacagttcca taaccatcct caaaatgtac tttttgtctg aatgtagact
    10081 atcacgggaa aactctctga acagttgacg cacatcaatc taactttcat tcgaaagtat
    10141 tttaatgttt gaacgaaaca gagaggcaac tatgattttc aatattcagc gctactcgac
    10201 ccatgatggc cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc tgggctgccg
    10261 ctggtgtcag aacccggaaa gccgcgcccg cacgcaagat ctgctgtatg acccacgact
    10321 ctgtctggaa ggctgtgagc tgtgcgctaa ggccgcgccg gaagtgattg agcgcgcgtt
    10381 gaatggtttg cttattcatc gagaaaagtt aaccccggag cattttacgg cgctaaccga
    10441 ctgctgcccg acacaggcat taaccgtgtg tggtgaagtg aaaagcgttg aggagatcat
    10501 ggcgaccgtt ttgcgggata aaccgtttta cgatcgcagc ggcggcggct taacgctttc
    10561 gggcggtgaa ccgtttatgc agccggaaat ggcgacggcg ctactgcaag ccagccacga
    10621 ggccggcatt catactgcgg tagaaacctg tctgcatgtg ccgtggaaat atatcgcccc
    10681 ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg acgcgccgtt
    10741 taaacagtgg accgacggta acgccgccag agtgctggat aacctgaaaa aactcgccgc
    10801 agcgggcaaa aaaatcatta tccgcgtgcc gctgattcag ggctttaatg ccgacgaaac
    10861 ctctgtaaaa gccattaccg attttgccgc cgacgagctg cacgttagcg aaattcattt
    10921 cctgccctac cacacgctgg gcatcaacaa atatcactta cttaatctgc cctatgacgc
    10981 cccggaaaaa ccgcttgatg cgccagaact gctcgacttt gcccagcagt atgcctgcca
    11041 gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa actggacacg
    11101 ctcagcgatc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa accgccggtc
    11161 tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga taagccgatc
    11221 ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat ctggatcaag
    11281 cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc gatcttcccg
    11341 gaatatactg tctcatggat cgaaaaagag atcgatgatc tggcagatcg tcccggtgct
    11401 ggctttgcgg tgagcgaaga gaacaaacgc gttctgcatg aagtgtgccc gtggtggcgc
    11461 ggtcagaccg tacaggatcg ctgctacggc atgtttaccg atgagcaaaa aggtctgctg
    11521 gcgaccggca tcattaaagc ggaaggcaat atgacctccg gcgatgcgca cctggcggtc
    11581 aatttcccgc tgctgctgga aaaagggctt gatggtctgc gcgagaaagt agcggaacgt
    11641 cgctcgcgca tcaacctgac ggtgctggaa gatttacatg gcgagcaatt cctgaaagcg
    11701 attgatatcg tgctggtagc ggtcagtgaa cacattgaac gtttcgctgc cctggcgcgt
    11761 gaaatggccg cgaccgaaac tcgcgaaagc cgtcgcgatg aactgctggc gatggcagaa
    11821 aactgcgatc ttatcgccca ccaaccgccg cagactttct ggcaggcgct gcaactgtgt
    11881 tacttcatcc agttgatttt gcagatcgaa tctaacggtc actcagtatc ttttggtcgt
    11941 atggaccagt atctgtaccc gtattatcgt cgcgacgttg aactcaacca gacgctggat
    12001 cgtgaacacg ccatcgagat gctgcatagc tgctggctga aattgctgga agtgaacaag
    12061 atccgctccg gctcgcactc aaaagcctct gcgggaagtc cgctgtatca gaacgtcact
    12121 attggcgggc aaaatctggt tgatggtcaa ccaatggacg cggtgaatcc actctcttac
    12181 gcgatccttg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac
    12241 cacgcgggaa tgagcaacga tttcctcgac gcctgcgtac aggtgatccg ttgcggcttc
    12301 gggatgccgg cgttcaacaa cgacgaaatc gtgatcccgg aatttattaa actcggtatt
    12361 gaaccgcagg acgcttacga ctatgcagcg attggttgta tcgaaaccgc cgtcggtggc
    12421 aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt gatgctggcg
    12481 gcgctggaag gcggtcgtga tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg
    12541 ttgtcggcgg gtaacttcaa caacttcgat gaagtaatgg acgcatggga tacgcaaatc
    12601 cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg ttgacaccat gctggaggag
    12661 aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg agcaaaaagt
    12721 atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggtcgg cattgccaac
    12781 ctcggcaaca gcctggcggc agtgaagaaa ctggtgtttg aacagggcgc gattggtcag
    12841 caacaactgg ctgccgcact ggcggatgac ttcgacggcc tgactcacga gcaactgcgt
    12901 cagcggctga ttaacggcgc gccgaagtac ggcaacgacg acgatactgt cgatacgctg
    12961 ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa cccgcgctac
    13021 ggtcgtggtc cggttggcgg caattattac gcgggtacgt cgtctatctc cgctaacgta
    13081 ccgtttggcg cgcaaactat ggcaacgccg gacggacgta aagcgcatac cccgctggca
    13141 gaaggcgcaa gcccggcctc cggtactgac catcttggcc ctaccgcggt cattggctcg
    13201 gtgggtaaac tgcctacggc cgcgattctc ggcggcgtgc tgctcaacca gaaactgaat
    13261 ccagcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct gcgcaccttc
    13321 ttcgaggtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga aacgctgctg
    13381 gaagcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc gggctattcc
    13441 gccttcttca ccgcgctctc tccagacgct caggacgata tcatcgcccg tactgaacat
    13501 atgctgtaat ctcctcagcc cggcggcggc gtcgccgggc taaatcattc acatcatcaa
    13561 tttcagcctc gctttcattc gaaatataat ttgtgctctg cgtcacattg ttattacact
    13621 gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga
    13681 catggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt ttatggcgca
    13741 atatcaggaa ctgaaaaagc gcggcattga gttcgttgtc gctagcggta atcagtatta
    13801 ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg
    13861 cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc gacatgaatc
    13921 gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct
    13981 gcaaagtgca tatgtcagcg aaaacgcccc cgaagcattc gtcgcactga tggcaaaaca
    14041 ctaccatcgc ctgaaacctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt
    14101 ttcgctcaac ctgccggatg aacaaatccc gttagtgatc gacaaactgc acatagcgct
    14161 ggatggcatt atgaaacccg ttaccagtgg ttttggcttt atcgacctga ttattcccgg
    14221 tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt cgccgcaaaa
    14281 tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc
    14341 ctttgcaatg gggaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga
    14401 taataatcat gaaggcgcgc tgaacgtgat tcaggcggtg ctggataaca catccccttt
    14461 taacagctga cctctctacc ggagccatgc gctccggttt tcccttcttt ttacatcctg
    14521 tacatca
//
LOCUS       DAYPHN010000123        14499 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000123 DAYPHN010000000
VERSION     DAYPHN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.123"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..264
                     /locus_tag="ACG0BW_003456"
     CDS             <1..264
                     /locus_tag="ACG0BW_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260381.1"
                     /translation="ATRAYFVSMFILPPCEEGTTIQIVWVWSGSPRVDRNIVLGLHLS
                     RPQSKPETGKLQAPAVILPFFSKIAVYLYSESCFGLLTPHLIN"
     gene            480..1598
                     /locus_tag="ACG0BW_003457"
     CDS             480..1598
                     /locus_tag="ACG0BW_003457"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFK7260382.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAINMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWTGE"
     gene            1664..1912
                     /locus_tag="ACG0BW_003458"
     CDS             1664..1912
                     /locus_tag="ACG0BW_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFK7260383.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1977..2360
                     /locus_tag="ACG0BW_003459"
     CDS             1977..2360
                     /locus_tag="ACG0BW_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFK7260384.1"
                     /translation="MDKQSPHETTKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPYSTVNQ"
     gene            2439..3092
                     /gene="nfsB"
                     /locus_tag="ACG0BW_003460"
     CDS             2439..3092
                     /gene="nfsB"
                     /locus_tag="ACG0BW_003460"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFK7260385.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3200..4447
                     /gene="ybdG"
                     /locus_tag="ACG0BW_003461"
     CDS             3200..4447
                     /gene="ybdG"
                     /locus_tag="ACG0BW_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFK7260386.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4515..5891)
                     /gene="pheP"
                     /locus_tag="ACG0BW_003462"
     CDS             complement(4515..5891)
                     /gene="pheP"
                     /locus_tag="ACG0BW_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFK7260387.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5993..9136)
                     /gene="cusA"
                     /locus_tag="ACG0BW_003463"
     CDS             complement(5993..9136)
                     /gene="cusA"
                     /locus_tag="ACG0BW_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFK7260388.1"
                     /translation="MIEWIIRRSVANRFLVLMGALCLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELSGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIIVIPILMGYWIRGK
                     IPPESSNPLNRFLIRAYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9148..10371)
                     /gene="cusB"
                     /locus_tag="ACG0BW_003464"
     CDS             complement(9148..10371)
                     /gene="cusB"
                     /locus_tag="ACG0BW_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFK7260389.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10387..10719)
                     /gene="cusF"
                     /locus_tag="ACG0BW_003465"
     CDS             complement(10387..10719)
                     /gene="cusF"
                     /locus_tag="ACG0BW_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFK7260390.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKITIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10743..12125)
                     /gene="cusC"
                     /locus_tag="ACG0BW_003466"
     CDS             complement(10743..12125)
                     /gene="cusC"
                     /locus_tag="ACG0BW_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFK7260391.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSHSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTTSSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12282..12965
                     /gene="cusR"
                     /locus_tag="ACG0BW_003467"
     CDS             12282..12965
                     /gene="cusR"
                     /locus_tag="ACG0BW_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFK7260392.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12955..14403
                     /gene="cusS"
                     /locus_tag="ACG0BW_003468"
     CDS             12955..14403
                     /gene="cusS"
                     /locus_tag="ACG0BW_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFK7260393.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALNNLLSNALRYTP
                     PGEAIVVRCQAVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERE"
BASE COUNT         3489 a         3910 c         3588 g         3512 t
ORIGIN      
        1 gccacaaggg catatttcgt cagcatgttt atattgcctc catgcgaaga agggactacc
       61 atccaaattg tttgggtttg gagtggcagc cccagggttg atagaaatat cgtcctgggg
      121 cttcatcttt ccagacctca gagtaaacct gaaaccggaa agcttcaggc acccgccgtt
      181 atcttaccct tcttcagcaa aatagctgtt tatttataca gtgagtcctg tttcggactt
      241 ctcactcctc atttgatcaa ctgaagctga acccgtattt ttctgcatgc ctcaatcgtc
      301 aaaattcttc gccaccagcc agctctgcca taacttgtca cattgcaaaa gtagccaaac
      361 ttcttcagtc acaatcagtt caccatcacc aaaaaaaaga agcattccag aacttccgca
      421 tctcgcccag ccgctccgac ttctatactg aatagaaaac gccaacataa gagaaaccta
      481 tgccattacc cgattttcat gtttctgaac cttttaccct cggtattgaa ctggaaatgc
      541 aggtggttaa tccgccgggc tatgacttaa gccaggactc ttcaatgctg attgacgcgg
      601 ttaaaaataa gatcacggcc ggagaggtaa agcacgatat caccgaaagt atgctggagt
      661 tggcgacgga tgtttgccgt gatatcaacc aggctgccgg gcaattttca gcgatgcaga
      721 aagtcgtatt gcaggcagcc gcagatcatc atctggaaat ttgcggcggt ggcacgcacc
      781 cgtttcagaa atggcagcgt caggaggtat gcgataacga acgctatcaa cgaacgctgg
      841 aaaactttgg ttatctcatt caacaggcga ccgtttttgg tcagcatgtc catgttggtt
      901 gcgccagtgg cgatgacgcc atttatttgc tgcacggcct gtcacggttt gtgccgcact
      961 tcatcgccct ttccgccgcg tcaccatata tgcaaggaac ggatacgcgt tttgcctcct
     1021 cacgaccgaa tattttttcc gcctttcccg ataatggccc gatgccgtgg gtcagtaact
     1081 ggcaacaatt tgaagccctg tttcgctgcc tgagttacac cacgatgatc gacagcatta
     1141 aagatctgca ctgggatatt cgccccagtc ctcattttgg cacggtggag gttcgggtaa
     1201 tggatacccc gttaaccctt agccatgcga taaatatggc aggattgatt caggctaccg
     1261 cccactggtt actgacagaa cgcccattta aacatcagga gaaagattac ctgctgtata
     1321 aattcaaccg tttccaggcc tgccgctatg ggctggaagg cgtcattacc gatccgcata
     1381 ctggcgatcg tcggtcacta acggaagaca ccttgcgttt actggaaaaa atcgcccctt
     1441 ctgcacataa aattggcgca tcgagcgcaa ttgaagcact gcaccgccag gtcgtcagtg
     1501 gtctgaatga agcgcagcta atgcgtgatt tcgtcgccga tggtggctcg ctaattgggc
     1561 tggtgaaaaa gcattgtgag atctggaccg gagaataagt cgggaattgc cttacagaag
     1621 aatatccctg acaatggtgt tttaacttac atttaacaat gttatgaaac accctttaga
     1681 aaccttgacc accgcagcag gcattttgct gatggctttc ctctcttgcc tgctgcttcc
     1741 cgcccccgca ttggggctga cgctggcaca aaaactggtg accacgttcc atctgatgga
     1801 tcttagccag ctttacactt tattgttttg tctgtggttt ttagtgctgg gcgctattga
     1861 gtattttgtt ctgcgcttta tctggcgacg ctggttctcg ctggcggatt aaacgtcgat
     1921 tatcgccgaa cttcgcggca tactttgctt attctccttc gcctaaagga acgtttatgg
     1981 ataagcaatc accgcacgaa acgacgaaac gcctggccct tgagttaccc tttgtcgagc
     2041 tttgctggcc ttttggcccg gagttcgatg tttttaaaat tggcggcaag atttttatgc
     2101 tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa gtccgatcca caaaaatccc
     2161 tgctaaatca gcaaatatac ccaagcatca agccagggta tcacatgaat aaaaagcact
     2221 ggatttcggt gtatcccggc gaggaaatct ccgaagcgtt acttcgcgat ctgatcaacg
     2281 attcgtggaa tctggtggtt gatggtctgg ctaaacgcga tcaaaaacga ctgcgtcctt
     2341 actcaaccgt aaatcaatag cgcatttttc tcgcttacca tttcatctcg atccttgtaa
     2401 tctgctggca cgcaaaatta ctttcacatg gagtctttat ggatatcatt tctgtcgcct
     2461 taaagcgtca ttccactaag gcatttgatg ccagcaaaaa actaaccccg gaacaggccg
     2521 agcagatcaa aactctcctg caatacagcc catccagcac caactcccag ccgtggcatt
     2581 ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc caaatccgct gccggtaatt
     2641 acgtgttcaa cgagcgtaaa atgcttgatg cctcgcacgt tgtggtgttc tgtgcaaaaa
     2701 ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca ggaagatgcc gatggccgct
     2761 ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg caagttcttc gccgatatgc
     2821 accgtaaaga tctgcatgat gatgcagagt ggatggcaaa acaggtttat ctcaacgtcg
     2881 gcaacttcct gctcggcgtg gcggcgctgg gtctggacgc ggtacccatc gaaggttttg
     2941 acgccgcaat cctcgatgca gaatttggtc tgaaagagaa aggctacacc agtctggtag
     3001 ttgttccggt gggtcatcac agcgttgaag attttaacgc tacgctgccg aaatctcgtc
     3061 tgccgcaaaa catcacctta accgaagtgt aattctctct tgccgggcat ctgcccggct
     3121 atttcccctc agattctcct gatttgcata accctgtttc agccgtcatc ataggctgct
     3181 gttgtataaa ggagacgtta tgcaggattt aatatcccag gttgaagatt tagcgggtat
     3241 tgagatcgat cacaccacct caatggtgat gattttcggt attatttttc tgaccgccgt
     3301 cgtggtgcat attattttgc attgggtggt actgcggacc ttcgaaaaac gcgccattgc
     3361 cagttcacgg ctttggttgc aaatcattac ccagaataaa ctcttccacc gtttagcttt
     3421 taccctgcag gggattatcg tcaatattca ggcggtattc tggctgcaaa aaggcaccga
     3481 agcggcagat attctgacta cctgcgcgca gttgtggatc atgatgtatg cgctgctttc
     3541 agtcttctcg ttgctggatg ttattttgaa tctggcgcag aaattcccgg cggcatctca
     3601 gttaccgctg aaagggatat ttcaggggat taaactgatc ggcgcgattc tggtcggcat
     3661 tttgatgatc tcgctgctga ttggtcagtc gccagcgatt ctgatcagcg gtcttggtgc
     3721 aatggctgcc gtgctaatgt tggtatttaa agatccgatt cttggtctgg tggcaggtat
     3781 tcagctttcc gcgaacgata tgctgaaact gggcgactgg ctggagatgc cgaaatacgg
     3841 cgcggatggc gcggtgatcg atattgggtt aaccaccgtc aaagtacgta actgggacaa
     3901 taccattacc actattccca cctggtctct ggtttctgac tcctttaaaa actggagcgg
     3961 gatgtcagca tctggcgggc gacgtattaa gcgcagtatc agtattgatg tcaccagtat
     4021 tcgttttctt gatgaagacg aaatgcaacg tctgaataaa gcgcatttgt taaagcctta
     4081 tttaaccagc cgccatcagg aaattaatga gtggaatcgc cagcaaggtt ctacggagtc
     4141 ggtattaaac ctgcgccgaa tgaccaatat tggaaccttt cgtgcctatc tgaacgaata
     4201 tctgcggaat catccgcgga ttcgcaaaga tatgacctta atggtgcgcc aactggctcc
     4261 tggtgataac ggtttaccgc tcgagatcta tgcgtttacc aacaccgtgg tgtggctgga
     4321 atatgaaagc attcaggctg atatattcga tcacatattt gcgattgtcg aagagtttgg
     4381 tctgcgactt catcagtcgc caaccggcaa tgatattcgc tctctggcgg gtgcatttaa
     4441 gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag cggacatcgt tttgtcggat
     4501 gtagcgtgaa tgccttattt ccgacgcagc gttttaaatg ccacaaacag gaatacaatc
     4561 cacaccggca gcaggatcgc tgacaagcgc atatcatcca tcgtgcacat cagcagcaaa
     4621 atcatgccga ggaaggcaat acaaagatag ttgccgaacg gataaagcag cgccttaaac
     4681 tgtgtttcac gtccctgacg tcgcatcgcc gcgcgaaaac gcagatgcgc caggcagatc
     4741 ataatccagt tcaacagcag cgttgctacc accagcgcca tcagcagacc aaacgctttt
     4801 tgcggcagca gatagttgat taacaccacc agcgaagtga tcgctccgga aagcatcagc
     4861 gagttaatcg gcacgccgcg acggctgacg cgagtcaaaa acttcggcgc attaccctgc
     4921 acagaaaggc caaacagcat gcggctgtta gagtaaaccc cgctgttata cactgacagc
     4981 gatgctacca gaatgacgaa gttcagcgca gaagctacca cgttgctgtc gagattatgg
     5041 aaaatcatca caaacgggct actgttggat ttcacttcca cccacggata gagtgccagt
     5101 aaaactacca gtgaaccgat ataaaacagc aggatgcgat acactacctg atttaccgct
     5161 ttcggaatgc ttttttccgg atcgcgcgct tcagcggcag taatcccaat cagctccaga
     5221 ccgccgaagg agaacataat caccgccagc gacaaaatca gcccattcca gccggtggca
     5281 aagaaaccac cgtagcgcca gaggttgtcg atactggctt tctcgccgcc gtgaccagaa
     5341 aacagcagcc acaggccaaa gccgatcata ccgatgattg ccagcacttt aatcagcgca
     5401 aaccagaact cggtttcgcc atataagcgc acgttcacca ggttaacggc gttgatgata
     5461 ataaagaagg cggcagccca aatccacgtt ggaacatccg ggaaccagta ctgcatatag
     5521 atgcccgcag cggtcagctc tgccattccc accagcacga acattaccca gtagttccag
     5581 ccagagagga agcccgcaaa cggtccccag tatttatagg caaagtgggc aaatgaaccg
     5641 gataccggct cctcaaccac catttcgcca agctggcgca taatcaggaa agcgatgatc
     5701 ccggcgacgc cgtagcccag caatacagcc ggacccgcca tctgaatcgc cgggccaatg
     5761 cctagaaaca gaccagtacc aattgcgcca cccagcgcaa tcagttgaat atgacggtta
     5821 tgtaatccgc gatgaagcgt cggctcttga ttcgacgcag tatcttccga tacggttgac
     5881 gcgtttttca cgcctttccc ctgtgtgtct tttttgttga ggggcacctt ttaacattta
     5941 gtgcccatcg tcgcaagaca caatccacac ggttaaaccg ggtatcctgc ttttatttcc
     6001 gtacccgatg tcggtgcagc cacatcagct tatacgccgc cgggataata aacagcgata
     6061 gcaaaggcgc ggtgatcatg ccgccaatca tcggcgcggc aatccggctc atcacctctg
     6121 aacctgctcc cgttccccac agaatcggca gcagaccagc gataatcacc gccaccgtca
     6181 tcgctttcgg gcgcacgcgc aggacagcgc cgtgatacaa cgcctcatcc agcttctgct
     6241 cgctgaatgt ttgcggatta ttcaacgacg gctcggcctc tatggcatga cgtaaataca
     6301 tcagcatcac cacgccaaat tcggcggcaa ccccggcaag ggcaataaaa ccggtgcccg
     6361 tcgccacgga aagatgaaag cccatccacc acagcaacca gatgccgccc accagcgcaa
     6421 acggtacgct gctgataatc agcaacgctt cgcccacccg acggaacgcc agatacaaca
     6481 gcacgaagat gatcatcaac gtcatcggca ccatcagttt cagcttatgg ttggcgcgct
     6541 ccagtagctc gaactgcccg gagaatgcca cgctggtgcc cggttttaac tgcacttttt
     6601 cagctatcgc tttttgcaga tcgtgaacca ccgacaccat gtcacgatcg cgggcatcga
     6661 tataaatcca gctcgtcggg cgcgcattct cggttttcag catcgacggt ccggtagaga
     6721 ctttaacgtc agccacgtct gccagggtga tttgctgctt catcggcgtc agaatcggca
     6781 actggcgcag cgcctgcgga ctatcgcgcc agctttgcgg ataacgcaga ttaattggat
     6841 aacgggcaat cccttccacc gtttcgccaa ccatcgcccc gcccaccgca gatgtcacaa
     6901 acaattgcac atccgccacc gtcataccgt aacgcgcggc tttttcacgg ttaatctcaa
     6961 cgttaatata gcgcccacct tccagacgct cggcaagcgc agaagctacg cctggcaccg
     7021 ttcgcgccac ttcttcaatt tgctccgcca tcgcgtcgat atccgccagc acagtgccgg
     7081 aaactttaat gccgataggg cttttaatgc cggttgagag catatcgata cggttacgaa
     7141 ttggcggcac ccacagattc gccagccccg gcagacgcac ggtgttatcc agttcttcaa
     7201 tgattttgtc catcgtcatg cctggccgcc actgatcttg tggcttaagc tggatggtcg
     7261 tttctaccat ctccagcgga gcagaatcgg tggcggtttc cgctttcccg gttttgccaa
     7321 atacccgcgc cacttcaggt acgctcataa tcagcttgtc ggttttttgc agcatactcg
     7381 ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat atacaacaag tcgccttcat
     7441 tgatctgcgg taaaaattcc ccgccaactt tattgagcgg ccagagaacc gtcagcaccg
     7501 aaagcgctgc caccagcagc gtggtttttg gccagtgcag tactttcagc aacagcggat
     7561 gataagcacg aatcaaaaag cgattgagcg ggttactgct ttctggcgga attttgccac
     7621 ggatccagta acccatcagg atagggatca ctatgatcgc cagcagcgcc gcacccgcca
     7681 tcgcatacgt tttggtgaac gccagcgggc caaacagacg cccttcctgc ccttccaggg
     7741 tgaagatcgg gataaacgac aacgtgataa tcagcagact gataaacaac gccggaccca
     7801 cttcaacaga cgcatcggtg atcacctgcc agcgcgtttt attatccagc gtggcgtcag
     7861 gatgctggtg ctgccactct tccagccgtt tatgcgcatt ctcgatcatg acgatagcgg
     7921 catcgaccat cgccccgacg gcaatcgcaa tgccgcccag cgacataata ttggcgttca
     7981 gtccctgaaa gtgcatgaca ataaaagcaa tacacaaccc cagcggcaac gaaataatcg
     8041 ccaccagcgc cgagcgcaca tgccagagaa acagcgcaca gactaccgcc accacaataa
     8101 actcttccag caacttgccg ctgaggttgt cgatggcgcg gtcaatgagc tggctgcgat
     8161 cgtatgttgt aactatctcc acgccttccg gcagactact tttcagcgtt tccagtttgt
     8221 ctttcacggc ggcgatcact tctcgggcgt ttttgccgga gcgcaggatc accaccccgc
     8281 ccgccacttc gccttcgccg ctcagttcgg caatgccccg gcgcatctcc gggccaatct
     8341 ggaccttcgc aacatcgcgc agataaacgg gcacgccatt ttcactggct tttaaaacga
     8401 tgtgattaaa gtcgtcgagc gtttgcagat agccgctggc gcgcaccata tattccgctt
     8461 ccgccagttc gatcgacgaa ccgcccgctt cctggtttga agcatccagc gcgcttttta
     8521 cttcggcgag actgatgcca tattgcgcca ggcgctgagg atcgataacc acctgatact
     8581 ctttcaccac accgcctacc gacgccactt ccgcaacgtc aggaatggtt ttcagctcat
     8641 atttgagaaa ccagtcctgc aatgagcgta aatcggcaag atcgtgctta ccgctgcgat
     8701 ccaccagtgc atattcatag atccagccaa cacccgtggc atctggcccc agctcggcgc
     8761 tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc cgcgagcgcg
     8821 cccagtatgg atcggtgcca tcttcgaaaa tgacatacac ataagagtcg ccaaactgcg
     8881 agaaaccgcg cacagtcttc gcgccaggca ccgacaacat agtggtggtt agcggataag
     8941 tcacctgatt ttcaacgatt tgcggtgcct gaccgggata gctggtttta ataatcacct
     9001 gtacatcaga gagatccggc agtgcatcca ctggcgtgtt aatgatggtc caggtgcccc
     9061 agatactcag acacaacgcg cccatcagca ccagaaaacg gttcgccacc gagcgacgaa
     9121 taatccattc aatcattggc tattccctca atgcgcatgg gtagcacttt cagagcgcat
     9181 ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc cgctggaaac
     9241 caccttttca ccttccgcca gaccagagcg taacgcggtg acgccttgcg atgcctggaa
     9301 aacggcaacg cgtttcggta caaagcgtcc gtcggcatca acggtaatca cccgctgttc
     9361 gttgccggta tcaatcagcg cctgtgacgg aatgagcagc atcggttcgc tggcggtgtt
     9421 gagttgcaac caggcgttca ttcccggttt tagcgcctcg tcggcgttgt cgacttccag
     9481 acgcagctgc agcgtgcggg tcgcggcatc cacgccgggt agcagggtcc atttgcgaat
     9541 ggtgagtgtt ttatcaggtc gcgccggaac ggtgagcgta aactgcgagg catctttcac
     9601 cagccaggcg atggactccg ggatcgcggc agtgatccac accgggtcca taccctgaat
     9661 tttcgctacc acattatctt tggcgatatt cattcccgcg cgcagatcaa acgcggtgat
     9721 cacgccatca atgggcgctt tgagcgtaaa gcgagtctgg attttttgcg tggcgatcag
     9781 gcggcgaata tccgcctccg gcattcccgc cagtcgcagc cgctcaagaa tgccttcagt
     9841 ctgggtcgcc gtaccgccgg tttcgcgcag cagtaaatac tcactctgcg cttccaccca
     9901 gtcgggaatg gtcaggtcga gaagcggtgt gcccttttgc actttatcgc ccacggtaag
     9961 cggatacacc ttgtcgataa acccggcagc gcgagcctgc acaatggcat actgatactc
    10021 gttgtaactg acattcgccg ggaaactctg ggcaaaagtc agcggcccgc gcgtgacggt
    10081 agccgttttc acccccagat tctgcgtctg agtcgggtca atgcgcacac cagacgcaga
    10141 actctcttca tcggcatatt tcggcaccag atccatatcc ataaacggcg atttacctgg
    10201 tttatcgaac cgcgtattgg gatacatcgg gtcgtaccag aataaaattt tacgttctgc
    10261 ggtcgacgtt ttttctgcgg gcggttccgc ctttgcaaac caggtaaaac ctgccgcaga
    10321 aataataccg cccgcgatca tgctgccgat aataagcgcg atttttttca tctcattaaa
    10381 cctgggttac tggctgactt taatatcctg taataaagaa aggttgccct gctggacaaa
    10441 attaaacgcc actttgtcgc cggttttaat ttcactcatt ttcgtctgcg gggtgatggt
    10501 aaagcgcatg gtcatctccg gccagttcac cgcggtaatt ggatcgtggt ggatggtgat
    10561 ttttttgctt tccagatcaa tggtttttac cacaccggtg gcgctaatga cctgtggttg
    10621 tgcctcgctc atggtttcat gatgatgttc gttagcctgg gcattaaagc caataacggt
    10681 aaacagactg aacattgcga cttgcagtgc ttttttcatt tattctctcc tggagttaaa
    10741 aattattgct gccaaccgcc gcctaaggcg gtatataaag aaatttcatt aacctgacgg
    10801 gcataattca ggtcaagtaa agtttgtcgg gttgcaaata aagaacgctc agcatccagc
    10861 acttccagat aacttaccgc gccgtgctga tataatgccc gcgcccgttg caaagtaatt
    10921 tgcagcgacg ccagataacg ctgctgggcg ctaatttgat cgttcaggct ttgacgtaat
    10981 gccagcgcat ctgccacttc tttaaaggcg ttctggattt tctgttcata attcaccacc
    11041 gactgctgct ggcgaatttc ggcgatatcc agattggcct ggttgcgtcc ggcattaaaa
    11101 atggggatct caattttggg aataaaattc cacatcccgc tgctggcgtt aaataatgac
    11161 gacagatcgc tgctggtggt cgatattccg ctggtcaggc tgatagacgg gaaaaatgct
    11221 gcacgtgcag caccaatatt ggcattagcc gccattaacg cgtgttcagc ttccataata
    11281 tccgggcgct gcaataaaat ttgcgacgac aagcccgccg gtaatttaac gctttgcagg
    11341 ctgtggctgt ttactgtctg cgcttgcggc agcttgccgt agcttcccaa taacagttgc
    11401 aatgcattat tcgcctgcgc cagttccccc tgacgtttag cgatgtcgct gcgggtactt
    11461 tcaatcaccc cgcgagcctg ttccagcgcc agaacattgc tgctaccggt caacagttgt
    11521 ttttcgacaa acgcatatga ctgctgataa ttacgcagcg tttcttcggc gatttgcaat
    11581 tgcgcatacg ccagctgctg attgaaatag ctttgcgcga cattagaaac cagcagaata
    11641 tgcaccgcgc gctgggcttc ctcagtggct aaataatttt gtcgctcggc ttcgctcatg
    11701 ttctttaagc gaccgaaaaa atcgagatca aagctggcgt taaggccagt cgagaactcc
    11761 cgcgtcgtgg ctgaatcgcc tttaagattg ccgctccagc tgccgctgcc ctcgccattg
    11821 agctgtgggt agcggtcggc atcggtcaga cgatattgcg cccgcgcttc ctgcactttc
    11881 agcgtcgcca tgcgcaaatc ccggttattc accagcgcct cgctaatcag cgtcttcacc
    11941 tgattatcaa caaaaaaggt gcgccagccc gcgttctggt agttatctgc tgcgttaacc
    12001 aggccgttct ggttgagtga gaactgctgc ggcacgggca ttgccgggcg ctgataatcc
    12061 ggtgccagtg aacaaccggt tagcgcaagg gccacacaaa atggcagaag tttacaagga
    12121 gacataggca cataatttct ggtgatttta tgtcgccaac tttacccgcc aggctctgat
    12181 tttccggtga caggaaaatg acaaaattgt cattttgcca ataagcgatt gccatctgat
    12241 cccgctactc tagaattgcc cgggcaacat gcggaggaaa tatgaaactg ttgattgtcg
    12301 aagatgaaaa gaaaaccgga gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg
    12361 tcgatttggc cgacaacggg ctgaatggct accatctggc gatgaccggt gattatgacc
    12421 tgataatcct cgatattatg ctgccagacg tgaacggctg ggatatcgtg cgtatgttgc
    12481 gttccgccaa taaagggatg ccgattctgt tgcttaccgc gcttggcacc attgaacatc
    12541 gcgtcaaggg gctggagttg ggggcagatg actacctggt gaagccattc gcttttgctg
    12601 aactgctggc gcgggtgcgc acattactgc ggcgcggggc ggcggtgatt atcgaaagtc
    12661 agtttcaggt tgccgatttg atggtcgatc tcgtcagccg caaagtcacc cgcagcggta
    12721 cgcgcatcac tctgaccagt aaagagttta ctctgctgga gtttttcctt cgccatcagg
    12781 gcgaagtgct gccccgctcg cttatcgcct cgcaggtgtg ggacatgaat ttcgacagcg
    12841 acactaacgc cattgatgtg gcggtgaagc ggctgcgtgg caaaatcgac aacgactttg
    12901 agccgaagct gattcaaacc gtgcgcggcg tgggttacat gcttgaggtg ccggatggtc
    12961 agtaagccat ttcagcgccc gttttcgctg gcaacccgcc tgaccttttt tatcagcctc
    13021 gccaccatcg cggcgttttt cgcctttgca tggataatga tccactcggt aaaagtgcat
    13081 tttgccgagc aggatattaa tgatttaaaa gaaattagcg ccacacttga acgggtactt
    13141 aatcaccctg acgaaacgca agcccgacgc ttaatgacgc tggaagatat tgtcagtggt
    13201 tattccaatg tgttgatctc cctggcagat agccacggta aaacggtata tcactccccc
    13261 ggtgcgcccg atatccgcga gtttgcacgt gatgccatac ccgataaaga cgcgcggggc
    13321 ggcgaggtgt atctcctttc cggcccgaca atgatgatgc cgggccacgg tcacgggcat
    13381 atggaacaca gcaactggcg gatgattaac ttgccggttg gcccgttggt ggacggcaaa
    13441 ccgatttata cgctctacat cgcgctttcg atcgattttc atcttcatta cataaatgat
    13501 ttgatgaata aacttattat gaccgcatcg atcatcagca tcctgatcgt ctttatcgtg
    13561 ctattggcgg tacataaagg tcacgcgccg atccgcagcg tcagccgtca aatccagaat
    13621 atcacctcga aagatctcga cgttcgcctt gacccgcaga ccgtgccgat tgagctggaa
    13681 cagctggtac tgtcgttcaa ccatatgatc gagcgtattg aggatgtctt tactcgccag
    13741 tccaatttct cagcggatat cgctcacgaa attcgtacac caattacgaa tctcataacg
    13801 caaaccgaaa tcgccctcag ccagtcacgc agccagaagg agctggaaga tgtgctctac
    13861 tctaatctcg aagagctgac gcgaatggcg aaaatggtca gcgatatgtt gtttctcgcc
    13921 caggccgata acaaccagtt aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc
    13981 ggcaaagtgt tcgatttttt cgaggcgtta gcggaagatc gcggcgtgga gttgcggttt
    14041 gttggtgacg agtgtcaggt tgcgggcgat ccgctgatgc tgcgtcgggc gttaaacaac
    14101 ctgctttcta atgccctgcg ttatacgcca cccggagagg ctattgtagt acgctgccag
    14161 gcggtcgatc atcaggtgca agttaccgtc gaaaaccccg gtacgcccat tgcgcccgaa
    14221 cacttaccgc gattgtttga ccgtttctac cgcgttgatc cttcccgcca gcgaaaaggc
    14281 gaaggtagcg gcattgggct ggcgatagtg aaatcgattg ttgtcgcgca taaaggcaca
    14341 gttgcggtaa cgtcagatgc gcgaggaaca aggtttgtga ttgtgctgcc ggagagggag
    14401 tgataggtca tataacgcaa tagatattat ttgaattgag aatttgatat ttcgccggaa
    14461 gaaaaagcgt aaaatccgtt tcgtgcagat ggtgagttt
//
LOCUS       DAYPHN010000124        14480 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000124 DAYPHN010000000
VERSION     DAYPHN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.124"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            66..422
                     /locus_tag="ACG0BW_003469"
     CDS             66..422
                     /locus_tag="ACG0BW_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFK7260394.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(473..1369)
                     /gene="yhaJ"
                     /locus_tag="ACG0BW_003470"
     CDS             complement(473..1369)
                     /gene="yhaJ"
                     /locus_tag="ACG0BW_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFK7260395.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1474..2175
                     /locus_tag="ACG0BW_003471"
     CDS             1474..2175
                     /locus_tag="ACG0BW_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFK7260396.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASSGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2198..2362
                     /gene="yhaL"
                     /locus_tag="ACG0BW_003472"
     CDS             2198..2362
                     /gene="yhaL"
                     /locus_tag="ACG0BW_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFK7260397.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTANNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2495..3805)
                     /gene="cyuA"
                     /locus_tag="ACG0BW_003473"
     CDS             complement(2495..3805)
                     /gene="cyuA"
                     /locus_tag="ACG0BW_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFK7260398.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3833..5164)
                     /locus_tag="ACG0BW_003474"
     CDS             complement(3833..5164)
                     /locus_tag="ACG0BW_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFK7260399.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5440..6804)
                     /gene="tdcG"
                     /locus_tag="ACG0BW_003475"
     CDS             complement(5440..6804)
                     /gene="tdcG"
                     /locus_tag="ACG0BW_003475"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFK7260400.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6876..7265)
                     /locus_tag="ACG0BW_003476"
     CDS             complement(6876..7265)
                     /locus_tag="ACG0BW_003476"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFK7260401.1"
                     /translation="MKKIIETQRAPDAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7279..9573)
                     /gene="tdcE"
                     /locus_tag="ACG0BW_003477"
     CDS             complement(7279..9573)
                     /gene="tdcE"
                     /locus_tag="ACG0BW_003477"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFK7260402.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIGIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9607..10815)
                     /gene="tdcD"
                     /locus_tag="ACG0BW_003478"
     CDS             complement(9607..10815)
                     /gene="tdcD"
                     /locus_tag="ACG0BW_003478"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFK7260403.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10841..12172)
                     /gene="tdcC"
                     /locus_tag="ACG0BW_003479"
     CDS             complement(10841..12172)
                     /gene="tdcC"
                     /locus_tag="ACG0BW_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFK7260404.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12194..13183)
                     /gene="tdcB"
                     /locus_tag="ACG0BW_003480"
     CDS             complement(12194..13183)
                     /gene="tdcB"
                     /locus_tag="ACG0BW_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFK7260405.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13282..14220)
                     /gene="tdcA"
                     /locus_tag="ACG0BW_003481"
     CDS             complement(13282..14220)
                     /gene="tdcA"
                     /locus_tag="ACG0BW_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFK7260406.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3753 a         3860 c         3381 g         3486 t
ORIGIN      
        1 tacgaatagc atttacacaa tacccggaat agactttcgt cacttttaat taaagggatg
       61 tttttatgca gtggtattta tccgtattaa aaaattatgt tggtttctct ggtcgcgcac
      121 gtcgtaaaga gtactggatg tttactctga ttaacgccat cgtcggcgct attatcaatg
      181 tcattcaatt gattttaggt ttagagtttc cgttcctttc tctcatttac ctggcagcaa
      241 caatcattcc ggtcattgct ctgtgcgttc gtcgtctgca tgatactgac cgttcaggtg
      301 cgtgggcgct tctctatctg gtaccgatca ttggctggtt agttctgttt gtatttgcct
      361 gtttagaggg caattctggc agcaatcgtt acggaaacga tccgaaattt ggttcaaatt
      421 aatattaaaa ttggggcgat atttcgcccc ttgttattaa caataacaaa tattattttc
      481 cgctaaaaag tttgggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      541 gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
      601 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      661 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      721 gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgccaccgca attccacgat
      781 atttcacgcg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      841 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      901 gaaaatgcat atccggcgcg ataacaatat ccgcccgccc ctgctccagc cgttcccacg
      961 cccccgccag cacttcggtg ataattgcca gttgggtatt ggcttttgcc gccagtttgt
     1021 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcctc ggtcacaatg gtgagatgcg
     1081 tttcccaacc gcgcgcgaga gcttccgcat cggtagtcag tttatctgcg gcttccagca
     1141 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
     1201 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
     1261 cggaaggcac gcgtcccagc tcatccgccg ccgccgcaaa actgccccgg cgatcgatcg
     1321 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
     1381 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
     1441 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
     1501 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1561 ttcgacccga aattgttagg ctatgcctcc ctgcgcgtgc ttaaccagga agtgctggcc
     1621 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1681 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag ctccggtgag
     1741 gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac
     1801 aaaccgttaa cgcgaatgca gctttggctg gatgcctgcc cgcagcgaga gaatccgctg
     1861 attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc agagggtgcg
     1921 atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct cgacaaaggc
     1981 gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa
     2041 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt
     2101 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     2161 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cggccaaaaa
     2221 gcgtcagcca gtgaagcccg tggtggcgaa agaacctgct cgcaccgcca ataattttgg
     2281 ctatgaagag atgttgagcg agctggaagc catcgtcgcg gatgctgaaa cgcgtttagc
     2341 cgaggatgaa gctaccgcgt aaagacaggt gccggatgcg gcgtaaacgc cttatccggc
     2401 ctacaaaacc caacaacatc aacaaattgt gcaattcgta ggccagataa gacgcgccag
     2461 catcgcatcc ggcacgatac ccaaaacctg gcgtttatct ggccttgctc gccataatct
     2521 cgataatctg ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa
     2581 tcgactgctc aacatcatgc gccacaatcc cttcattgcc ggtcacggcg gtatcatcca
     2641 gcgccattaa caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc
     2701 tgttcgacgc accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca
     2761 tcgaaatggt ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg
     2821 ctgcggtcgt tgcggcacaa agcgcagaca aacgcggtaa ctggttatgg atgtaaatcg
     2881 cgctcaaatg cgaaagcatc agcgcacgcg ccagccgttc atcatccgct ccgaagtgtt
     2941 ctgctaccac caccacaggc attgttgcgg tgatcccttg gttacccgag ccggagttac
     3001 tcattgccgg aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca
     3061 caatggatga agagagatct ttcgccagca aaccgcgcgc gcactgtttt tccagcgtcg
     3121 caccaatatg cagcccccag ttaccgctca aaccttcctg cgataacgca caatttaact
     3181 tcgcagaatc gagaataaag cggatcgccg caaacgggac ttcattgacg aacttcagga
     3241 tctcagccag cgtcgtcctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgtca
     3301 cacacgcctg ctgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat
     3361 gcccgccgac aatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga
     3421 agaggatttc atcgcaaggt tcctggatct taactgagac tttccccgcc gccagcagtg
     3481 ctttggcatc ggcaattgcc tgcgcagttg catctttcag cacttccagc ccggcgttag
     3541 catttccacc taacgctccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg
     3601 gaacggtgac gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt
     3661 caaccggacc ttccagttca gccgcagcaa ccgccgctgc cagcgccagt gaaatcggtt
     3721 cagtacaccc cagcgccggt tttacctcct cctgaacggc gaggatgtaa cgctgccata
     3781 acggatttaa agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa
     3841 atgccaggaa cggagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca
     3901 tccctttgta tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc
     3961 ctaccatgcc gaaaatcggg ctacagatag aggtgaagct caacaccggg gcgttcagta
     4021 cgatggcgct ccaggccagc aaaatggcga aaatcatgat gccgcgctga acgagatttt
     4081 cgttaatctt ctcggcaggt atcttgcgac gcaggatgtt cattacgatc ccttgcgttg
     4141 cttcgcgaaa gcctaaatag acgccaaaga atgcagtcat tacagcaaag atattgagaa
     4201 tgacgctgac cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg
     4261 ccaacgcgga aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg
     4321 tgaacgacac ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg
     4381 cccgcaatgc tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca
     4441 ccatcgggct taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa
     4501 tggcgttttt caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca
     4561 ttcccaccat cgacacgccc agcgccgcga ccaccagcag cttggtcagc accatgcccg
     4621 tcgaaatttt gaataacaat ttctcgccgc gtgaggagat cgccaccaga atgcaaatca
     4681 gtaccagacc ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca
     4741 ggtaggaagc actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca
     4801 ttacgaaata gagtgcacct aacaggatgc cccagttttt acctaaatag ccgctaatga
     4861 cgctcgggta atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa
     4921 acagatacat tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca
     4981 aaccgacctg caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa
     5041 tcacccagcc ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca
     5101 ttccagcacg acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt
     5161 ccataatttc tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg
     5221 cgatatgcgg tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag
     5281 gtcagagaaa atcttcacaa tcaagacttt gaaataacgg agatggataa gaattttcta
     5341 ctaaattaat cgcaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaaggt
     5401 gcacatttgt gcacccaatg atgaaagctg acagtaatgt cagccgcaga ccactttaat
     5461 ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata
     5521 catcgtctcg atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat
     5581 ccgtgcggca tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg
     5641 tacctgtccg gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc
     5701 cgcattgcac acctgcgccg gactgccgcc caggagttca gttaaccctg ccgccgccat
     5761 tgaacaggcc acgccaatct ccccctgaca gccgacttcc gcgccagaga tggaggcgtt
     5821 cattttatac agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc
     5881 gtttaccgga cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc
     5941 accgttagtc ggtgccgtta ccacacgccc gccagctgcg ttttcttcac taaccgccag
     6001 cgcgtacata ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga
     6061 aaccagctga cgacgcagcg ctacggcacg acgcggcaca ttgagcggac caggcagcac
     6121 gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa
     6181 ccctgcgtca atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcagaccaga
     6241 tatagacagg ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta
     6301 aggtacggac gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc
     6361 gccgccgaca gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat
     6421 ccgcattccg ttctcatggc gaggcaacat ttcgggatgg aagataatgt tttttgctac
     6481 aggaaaatca acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa
     6541 ctctataaat gcagggatct catcaatgac aacatcctgc ggactatttc ctgccagtcc
     6601 catgatgatg gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag
     6661 atcgaccaca atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa
     6721 actttttccg gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt
     6781 gaaaatatcg aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag
     6841 cggaacgacc ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatggct
     6901 tcaatttcca gcttcacatc tttcggcaaa cgcgcgacct gtacacagct ccgggtcggg
     6961 taggtcgcct gatgctcatc gaagaactgc ttatagactt cgttgatggt ggcaaaatca
     7021 ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca
     7081 accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg
     7141 atctcaccgg tctgtgggca aaccggaatt tgaccggagg tgaagaccat gctgcctaaa
     7201 tcaacgccct gaacataagg gccgattgcg tctggggcac gttgcgtttc gataatcttt
     7261 ttcatacatc ctccggcgtc agagcgcctg ggtaaaggta cgtgaaataa catcctgctg
     7321 ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt
     7381 aggatatttt tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac
     7441 gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc
     7501 gacaaggttg gttttacgta ctggatcttc tttgcccagc gccgcaggca cgatcgagaa
     7561 ggtgtacgag atcccgtctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga
     7621 ggccacggca cctttgcggt cacgaccgtg catcgggtta gcgcccggtg cgaacggtgt
     7681 acccgcacga cgaccgtctg gcgtgttacc ggttttctgg ccgtacacca cgttagaagt
     7741 gatagtcaga atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt
     7801 cttcataaag cgttcaacca ggtcgcaggc aatactgtct acgcgctcgt cgttgttgcc
     7861 gtactgcgga tattcaccgt cgatttcgaa atccaccgcc aggccgtttt cgtcacggat
     7921 tggtttcacg cgggcatatt tgatagcaga cagggagtcc gtcgccaccg acaggcccgc
     7981 gatgccgcac gccatggtgc gatagacatc acgatcgtgt agcgccatca gcgaagcttc
     8041 gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc
     8101 cagccagtcc atgaagtgat cgagactgtc catcactttg tcgtaatcca gcacgtcgtc
     8161 catcagcggt gccgttttcg gaccgacctg aatcttcagc ttctcatcca ccccgccgtt
     8221 aattgcgtac agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt
     8281 accaatcacc attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt
     8341 acgcatcaga tcgtcgtttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc
     8401 atattttttg aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg
     8461 tgccggcccc atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt
     8521 acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa
     8581 cagcgaatca aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg
     8641 atcgatcagt tcctgtgcct gctgctcatt gagtacgcca gctttaaagt cgcgctcaat
     8701 gtagatatcg aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt
     8761 cactgccgcc agataagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg
     8821 gcgagagata tcaaagccat atttcgccgc catttcctga atctgcaaaa gcgcatgacg
     8881 atgctctgcc agctcctcac gcagacggat agtggcttcc agatcctcgc ctttttccag
     8941 acgggactgg agatcggcaa attgcagttc gcgttcccgc accagataac tgatgccata
     9001 cagcgccacg cggcggtagt caccgataat gcgcccacgg ccataaccat ctggcaaacc
     9061 agtcagtaca cccgatttac ggcagcgcag catatctggt gagtaaacat caaatacgcc
     9121 ctgattatgg gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg
     9181 gccataggcg tggaatgaac ttttaatcat attaatgcca ccgaacggat gcagcgcacg
     9241 ctttaatggc gcatccgttt gcaggcccac tattttttcc agcggctggt taatatatcc
     9301 cgcatcatga gcggtaatgg tggtagcaat attagtatcg aaatcaaccg gcgcgtgggt
     9361 tgcgttttcg ataccaatgc cttccatcac tttttcccac aattccgtgg tggctggcgt
     9421 cgcttcagcg aggaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc
     9481 gcggacatta atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta
     9541 cagcttatcg ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca
     9601 actaaattat gcaaattctg cgggcgcgtt aactttgcct aaatgaatgg catccaaagc
     9661 aatcattttt tcttcgttgg ttgggataac ggcgcaaatg acacgcgcat tttcactgga
     9721 aacaattcgc tctccacagg agttagagcg attattcatt tctgtatcaa tctctacgcc
     9781 taatacagcc aaatgttcca tgaccagacg acgaattaaa cttgaatttt ctcctattcc
     9841 gccggtgaat ataattccat ccaggcgacg taatgaggct gcgtgtccgg caatatgacg
     9901 ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca
     9961 ggctttttcc agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc
    10021 tttattcact acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga
    10081 catcgccccg aagtcgacat caccactgcg ggtacccatc atcaagcctt ccagcggcgt
    10141 cattcccatt gaggtatcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt
    10201 gccaagatgc gccacaacca ggccggagtc atcttccgcc agattcagca aagaatgcgc
    10261 gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc
    10321 ttcataatat ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa
    10381 actggtatca aataccgcta cctgagttac gcccggaaat aattgctgcg ccgattcaat
    10441 accacttaaa ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc
    10501 aatgacttca tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg
    10561 gtggccaatt aaggccacac tgtcatttaa attccgtttt tccagttcaa atgcaattgc
    10621 cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga
    10681 taagaatgca ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc
    10741 gctggcatcg agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac
    10801 cggaaattca ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga
    10861 tgttcaggat ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt
    10921 atttcgccag agacggcgct ttacggatgg catacatcgg caacaggcac agcagggatg
    10981 cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa
    11041 caacccaggt ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca
    11101 ctttggtttt gtcaccttta taaccgaact tcagaatcag gccattcaag ccttccagcg
    11161 tccccagata gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg
    11221 catattccag tgtaatcgcg aacgttgttt tggtaccggt catggacgca aagtggttag
    11281 ccagataaga aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca
    11341 gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac
    11401 gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact
    11461 cttcacgttt agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca
    11521 tgatggaaat ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg
    11581 aaccgaggtc aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa
    11641 tcagcaccag gctggcgata aacggccata ccaggtagct catcacttta accatcagat
    11701 ccttaccaaa ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc
    11761 gattcagcgg tgcaaagccg agctggtttt cccagaacgt cataaaggta ttggtaatag
    11821 taacgccata aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca
    11881 cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag
    11941 agccagaaag acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca
    12001 ataccaacat cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca
    12061 gcaccccggc gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac
    12121 gccaggacga ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc
    12181 ctcaacgaat taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga
    12241 tattgccgcc ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac
    12301 cgcttaataa tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct
    12361 gaattaaggc aatcatactg tttctgattt catcttcgct gaccagcacg atgtcatcga
    12421 ctaattcacg aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg
    12481 ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc
    12541 cgtgaacgtt ttctgactgt acgccaataa cacgaatggt cgggttaata gattttattg
    12601 ccaccgcaat accagcaatt aaaccgccac caccaattgg cacaatcacg ttatcgacat
    12661 catagagatc ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat
    12721 catcgtaagg tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcga
    12781 tagtgtcgtt gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg
    12841 ctactttgga tttcggcgcg ccttttggca tcaccacttt accgtcgata cccagcatcg
    12901 cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga gcaggccact acacctttac
    12961 gtttttccgc atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac
    13021 cggtacgctg catgttttcg aatttgagga atatttcacc tttgcaacgt tcactaaaat
    13081 agttggagcg aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttagcttcaa
    13141 tgatgtcatc aatagcaacc gggagatcgt atgtaatatg cattataaaa cctcttagcc
    13201 tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta
    13261 tgtttaaata aaaacaaata actaaccaac ttcaattaat tgccttcgtc tacacccatt
    13321 ataagatgaa tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata
    13381 atttttcgac cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt
    13441 aataaattga tttgaaccaa aaggtgatgt catatcacaa ggaattacag ttaagaaatc
    13501 agcattgaga acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat
    13561 actgatgcca tttctttgta acgtggtgag cagctcgctg tagtacccca tattggtttg
    13621 tggcaacacc cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg
    13681 ggacttactg gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg
    13741 aagcttcatt tctgcgctta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc
    13801 cggtaagaac gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc
    13861 tttgaacttg ttgatcatcc cggacataaa agtaaaacca atcaatgaag gaaaaccaaa
    13921 tgagacttcc accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat
    13981 ttcacgggta atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac
    14041 accggtattt ttccgcacca ctaattccac gccaaaataa tcttcaatat cgttaatgat
    14101 tttactgaca gccggttgag ttaaccctaa ttcttttgca gccgagccga tagaaccact
    14161 tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat
    14221 aacgacctac gttaattacc tcattgacgg catgaagtgt atcaaaatga aatgaacagg
    14281 atatgtgcga ccactcacaa attaactttc aatactttcc atggtatcgt tattccaatt
    14341 aaatatcttt atatatcaat aagttaaagt ataaaaatca gcataaaccc tgatttttgt
    14401 caaaaaaaat gacggggata accatatttt atggcgataa catcattcgt tatgtggtta
    14461 atacaaaaaa ggggctgaga
//
LOCUS       DAYPHN010000125        14450 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000125 DAYPHN010000000
VERSION     DAYPHN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.125"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            162..1940
                     /locus_tag="ACG0BW_003482"
     CDS             162..1940
                     /locus_tag="ACG0BW_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFK7260407.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1980..2459)
                     /gene="xdhC"
                     /locus_tag="ACG0BW_003483"
     CDS             complement(1980..2459)
                     /gene="xdhC"
                     /locus_tag="ACG0BW_003483"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFK7260408.1"
                     /translation="MNHSETITIECAINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2456..3334)
                     /gene="xdhB"
                     /locus_tag="ACG0BW_003484"
     CDS             complement(2456..3334)
                     /gene="xdhB"
                     /locus_tag="ACG0BW_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFK7260409.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLVELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3345..5642)
                     /gene="xdhA"
                     /locus_tag="ACG0BW_003485"
     CDS             complement(3345..5642)
                     /gene="xdhA"
                     /locus_tag="ACG0BW_003485"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFK7260410.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAEPIHNGGN
                     LLKQSTMTTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLSGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTIDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6058..6810
                     /gene="actS"
                     /locus_tag="ACG0BW_003486"
     CDS             6058..6810
                     /gene="actS"
                     /locus_tag="ACG0BW_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFK7260411.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6889..6962
                     /locus_tag="ACG0BW_003487"
     tRNA            6889..6962
                     /locus_tag="ACG0BW_003487"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Gly,seq:ccc)
     gene            7195..8805
                     /locus_tag="ACG0BW_003488"
     CDS             7195..8805
                     /locus_tag="ACG0BW_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFK7260412.1"
                     /translation="MKGKSTLTLLLAGIFSCCTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQENSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREMSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            8871..9050
                     /locus_tag="ACG0BW_003489"
     CDS             8871..9050
                     /locus_tag="ACG0BW_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFK7260413.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAVITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            9396..10145
                     /locus_tag="ACG0BW_003490"
     CDS             9396..10145
                     /locus_tag="ACG0BW_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7260414.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            10142..11845
                     /gene="sctC"
                     /locus_tag="ACG0BW_003491"
     CDS             10142..11845
                     /gene="sctC"
                     /locus_tag="ACG0BW_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFK7260415.1"
                     /translation="MKIKLRITIILISALCIFNGLLTPGAYAATANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFASRNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            11850..12995
                     /locus_tag="ACG0BW_003492"
     CDS             11850..12995
                     /locus_tag="ACG0BW_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFK7260416.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLKNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNSNKKQNKKPWKKVMFNV"
     gene            12988..>14450
                     /locus_tag="ACG0BW_003493"
     CDS             12988..>14450
                     /locus_tag="ACG0BW_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFK7260417.1"
                     /translation="MFNKVLIGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGVEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NNNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSV"
BASE COUNT         4198 a         2916 c         3359 g         3977 t
ORIGIN      
        1 ttgatataga aatgtgatat ctcttccata atttcattag aaagtgataa aaatcttatg
       61 tttttatcaa agccaatttc gcctgagtta atatcatcat gatctatgaa ccatcaacgt
      121 cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc tatggagctt gctactacgc
      181 agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt tgccagaatg cttgccagcg
      241 ttttgcagct tgaggttgag atcgttgatg aaaacttgtg tcgcgtcgcc ggaacgggcg
      301 cgtatgggaa gtttcttggt cgccagttga gcggcaactc acgcctgctc cgccacgtcc
      361 tggaaacgaa aactgaaaaa gttgtgacac agtctcgctt cgatcccctt tgtgaaggtt
      421 gcgatagtaa agaaaattgc cgcgaaaaag catttctggg tacgcctgtc attttacagg
      481 atcgttgtgt tggggtgata agtttgattg ccgttaccca cgagcaacaa gagcatatca
      541 gtgataattt acgcgaattt tctgattatg ttcgccatat atccaccatt tttgtttcga
      601 aacttctgga ggatcagggg ccaggagata acatcagtaa aatattcgcg accatgatcg
      661 ataatatgga tcagggcgta ttagttgttg atgatgaaag tcgggttcag tttgttaatc
      721 agactgcctt aaaaacactt ggtgttgtac aaaataatat tattgggaaa cctatccgtt
      781 tcagaccatt aacatttgag agtaatttta cccatggaca tatgcagcat attgtttcgt
      841 gggacgataa aagtgaatta atcattggtc aattgcataa cattcagggc cgacaattat
      901 ttttaatggc gtttcaccaa tcgcatacca gtttttctgt agcaaatgca cctgatgaac
      961 cgcatattga acaattggtt ggcgagtgcc gtgttatgcg gcaattaaaa cgactcatta
     1021 gccgtattgc acccagccca tccagcgtta tggtggttgg tgaaagcggc acgggtaaag
     1081 aagttgtcgc ccgtgcaatc cataagttga gcggaagacg gaataaaccc tttattgcta
     1141 tcaactgtgc cgcgattccg gagcagcttc tggagagcga actgttcggt tatgttaaag
     1201 gcgcatttac tggcgcttct gccaacggta aaacagggtt gattcaggcg gcgaatacgg
     1261 gcacgctgtt tctcgatgaa ataggtgata tgccattaat gttgcaggct aaattactgc
     1321 gcgctattga ggcgcgtgaa attctgccga ttggtgccag tagcccaata caagtcgaca
     1381 ttcgcatcat ttctgcaact aaccagaatt tggcccagtt cattgccgaa ggtaaattcc
     1441 gcgaagatct cttctaccga cttaatgtta tcccgataac tctgccaccg ctgcgtgaac
     1501 gtcaggaaga tattgaacta ctggtgcatt actttttaca tctgcatacc cgtcgtctgg
     1561 gatcggttta tcctggcatt gctcccgatg tcgtcgaaat attgcgtaag catcgttggc
     1621 ccggaaacct gcgcgagtta agcaatttga tggaatatct ggttaacgtt gttccttcag
     1681 gtgaagttat cgacagcacg ctattgccac caaatctgct gaataatggc acaacggagc
     1741 aaagtgatgt aacagaggtc agcgaggcgc acctggcact cgatgatgcg ggcggcacgg
     1801 cgctggagga gatggaaaag caaatgatcc gcgaggcgct ttcacgtcat aacagcaaga
     1861 agcaagttgc tgatgaactg ggcatcggca ttgctacgct ctatcgcaag attaagaaat
     1921 atgagttgtt aaacacataa cctcagtgaa tccggcctga attcaggccg gatatccttt
     1981 tacttcgttt tctcgcaatc cagaactgta tttacaatca tctgataccc cgtgcagcga
     2041 caaagatttc ctgccagtcc gcgacgaatt tccgtaatgg ttaatggctt ctcgcgtggt
     2101 ttcgccagca ttgctgtggt agccataatc aggccaggcg tacaaaaccc gcactgcact
     2161 gcgccggatt tcgcataagc ctgctgaaca tgagaaagtt ttccgccttt cgcttcacct
     2221 tccagcgtgc ggatctcttt tccttcagcc caggcggcaa ggtataagca actgtctatt
     2281 gctgtgccgt cgaccagcac cgtacaggca ccacattcac ccacgcagca cccttgtttg
     2341 acacttagca gtccttgttc gcggagtaat tccgagagcg gcgtgcctgg tgcggcgtga
     2401 agctgaaaag ggatcccgtt aatggcgcat tcgatggtaa ttgtttcgct gtgattcatt
     2461 gcaattttcc ccccgccgcg gcgacggctt cgctaatcac ttttttggtc atcgtctgga
     2521 tgagatgcag acgaaactct ttactggccc gccatgaaga acgcggggcg acatcttgca
     2581 ggacagattc gctgatagct tccagcgttt gcaggtttaa tggcgcattt tgtgcagtct
     2641 gttcggcatg ttggcagcga atcggcgttg gcgcggcaac accaaatgcc agacgtaatt
     2701 cgctgaaatt gccgttatcc agtcggcaat gtgcggcgca gccaatcgtt gaaatatcca
     2761 ttgcgtcgcg catggcatat ttaaaatgcg cgctgcccgc gtgttctttc ggctggggcg
     2821 gaaaatgaaa ggcgacaagg atttcgtcat gctcaagaga cactttgccc ggcccagtgt
     2881 gaaagccatt aatcgggacg aaacgaacac cgcgcgggga gtgaatctcc agtttcgcgt
     2941 cataaattag cgttggcgtg gcagaatctg cgctggtagc accgttgcaa atatttccac
     3001 cgtaggtagc gacgttacgg atctgcggtc cggcaatgga tgaggccgca gcacataacg
     3061 ctgggagatg acgttgagtt acaggatctt caatgagctg ggtaaatgtc gttgcagagc
     3121 cgatacgtag tgagccatct tccgccagcg taattccccg cagctccacc agattatgaa
     3181 tatcaacaat atggcgataa cggtcattgt gatggtggag ctgaatcagt acgtcagtgc
     3241 caccggcgag cagtttggcc tgcgggttgt cagccagcag gttgatggca tcggcaaggg
     3301 ttgctgcgcg atggtaagaa gcaaaatcaa acatgatgtt atcctcaaat caatcctgcc
     3361 agatggaact cttcatataa ccgttttggc gtcagcggca gtgtattgat tgcaacaccg
     3421 gtagccatct tcaccgcgtt acgaatagca gcggcaacag gaattattgg tggctcaccc
     3481 agtgacttat gtccgtatgc ggattgcggc tcattgattt cgacgaacgc gctttccagt
     3541 tgtggcagat ccggcatggt cggcattttg taatccagca ggttggggtt acggaccacg
     3601 ccgcttttcg catcaatgat catctcttca aatagcgccc agccaatgcc cattcccatt
     3661 ccgccgtgta cctgaccttc tgccagtagc ggattaagaa tatggcccga atcatgaacg
     3721 ttgaggatgc ggttgatggt gactttgcac agcgcaatat cgatcgtcag atcaacaaag
     3781 gtacagccaa acgctggtgg gttagtggtg gttttgatgg agctttcggc agagagctgc
     3841 ccaccgcgtt cagggtggta gaaagcgtcc atcgccaaat cttttaacga cattaacggc
     3901 tcttccggtc gttcaaccag cacgatatgg ccttttatca aggtaagatt catcgctgac
     3961 tgatgtagca tgacagcggc gtgagcgatg attttctctt ttaataacag tgccgcactg
     4021 cgcagcgcgg gcgcggcaac atagctctgg cgcgaggcaa atgcaccggg atcgaacggc
     4081 gtaacgtcgg tatcttgtgt tgaaataacg cgaacgtcgc tgaccggaac ccccacggtt
     4141 tctgccacca tttgcgagaa cacggtgtcg gcaccctggc cgatttccgt ggcaccactc
     4201 tgtacgttga tggttccatc ctgattcatc aacaggcgcg cgccagcaat ttctacgccg
     4261 acaggccagg tgttagaggt gtagctaaaa caggcgacgc caacgccgcg gcgtaaattg
     4321 ccttgctggt tctggcattc tgcacggcgt ttttcccatt caaagatttt ccggcctttt
     4381 tcaagacact ccggcaaccc tgcgctgtaa atacgtttgc ccgagagcgg attagcatct
     4441 ccttcgcggg cggcgttgcg taaacgaatt tcaacaggat caatacctaa cgctgtcgcg
     4501 gcgtcatcaa gcatagactc aacggcaaat acgacttgtg gcgcgccata accacgcatc
     4561 gcaccagccg aggggaggtt ggtatagcag gtctttgaac tgtaagcgta ggcacaacga
     4621 ggataaaggt aagcgacttt attcccccct gcagaagcga tggagtgccc gtgagatgca
     4681 taagcgccgg tgttagacag aacatccaga ctataacctt tcaatgttcc gtcgcggttc
     4741 acgcccattt gcccgtcaat agtaaaagcg tggcgggtac gggttgcgag gaaacactct
     4801 tcacggctaa gggaaacttt caccggaatg ccgccaagct tgctggtcag gaatgctgcc
     4861 attggctctt ccagtacatc ctgtttatta ccaaaaccgc caccgacaaa cggtttgatg
     4921 actcgtacgc atgaccaggg aatattcagc gcctgaccaa ccacacggcg aacaatgtgc
     4981 gggatctggg tgctggaaac gatggtaatt cgcgagtcat cctccatcca tgccagcgat
     5041 gtcacgcttt ccatgtgaca atgttgaatg accggcgtct gatagtgccc ctgtacctgg
     5101 tagtcggcgg catcgattgt ttgttggaca ttgcccgtcg tcatcgtgct ttgttttagt
     5161 aaattgccgc cattatggat tggttcagcg tcatcagcca gcgccgcttc tggggtggtg
     5221 ataacgggta attcttgcca ctcaatgctg accaattgcg ccgctttttc tgccgtgagt
     5281 tcatcacggg ccacgacgat ggcaacggcg tcaccatggt gacgaacatg gcgagttagc
     5341 agggcacgat cggcggtatc gcgcttgttt tcgtcaagtg tccaggcatg cccagctgta
     5401 gcgaatggga tatcaggcac atcttcccag gtaaaaatcg ccagtacgcc cggtaaactt
     5461 ctggcttgtt catcattaat acttacggca taaccatgtg cgatagggct acgtacatat
     5521 ttcgcgtaac acatgcccgc cataacataa tcgtcagtat atcgtgcccg cccggtgacc
     5581 ttagcaatgg catctacgcg catgcatgat tcacctgtag cggttgcttc ccgcgcttcc
     5641 atagaaatcc cctcgatttg aacatttggt tcttatggca aagatttagt ctaagcaggc
     5701 aagatttacg ccagaaacga cgataagcgt taagggagga aggaagtgtg atgatgttaa
     5761 aaaaacacag atatgatacg aaacaggacc ctgaataggg gcagtgatac gtatcaatat
     5821 gagtggcgat aaaatcaatg tgataaatca ggattattac gcatcaggat gggaaatagg
     5881 gtaaaaaagt caggaaattc gatacctctg cagcgaatat tttgctgatg atacttccct
     5941 ttgccaaatg aaattaacct ctttctctat taccgcgttt ttccagaagc aagagattgg
     6001 gtatccagag gctggctgct atgatatggc gtcttgtttt ttaagcgagg aaagattttg
     6061 agtgcgggac gcctgaataa aaaatctctg ggtatcgtga tgttgttatc ggttggactg
     6121 cttttggcgg gctgctcggg tagcaaatca tccgatacag gaacgtattc cggctccgtt
     6181 tacaccgtga aacgggggga tacgctatat cgtatttcgc gcaccacggg aaccagcgta
     6241 aaagagctgg cgcgactgaa cggcatttcc cccccttaca ccattgaagt tggtcagaaa
     6301 ctaaaactgg gtggggcgaa aagtagcagt acacgtaaat caaccgccaa atcaacgacc
     6361 aaaaccgcat cggttacacc gtcatcagca gtaccgaaat cttcctggcc gccagtaggg
     6421 caacgttgtt ggttatggcc aacgacaggg aaagttatca tgccgtattc gacagctgat
     6481 ggcggcaata aagggattga tatctcagct ccacggggta cacctattta cgccgcgggt
     6541 gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct acggtaatct catcatgatt
     6601 aaacacagtg aagattacat tacggcttac gcccataatg acacgatgct ggtaaataat
     6661 gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg ggagcactga tgcggcatcg
     6721 gttcgcctgc atttccagat tcgttaccgt gctacggcaa ttgatccgct gcgttacttg
     6781 ccgcctcagg gcagcaagcc aaaatgctga tggcgaatta atcagcagtc agcagcgcgg
     6841 cacttgctaa ggagagcgta aggtttataa tgccttacgc atctcgaagc gggcgtagtt
     6901 caatggtaga acgagagctt cccaagctct atacgagggt tcgattccct tcgcccgctc
     6961 caatttatct tcacctcatc aacctgccat ttttgttgta tctgattttc tatattatct
     7021 taattcaatc gcttcagact tttcgcaatc gccgtaatac ttccccattt ttttgtaaat
     7081 aaaagcgttt acgcaatgca atccatttgt aacaacccag gtaaaatacc ttattttcaa
     7141 gttgttaaat aagtggttat ctacgctttt aacaaacgat tgatggaatc ttttatgaaa
     7201 gggaaatcta cattaactct tctgctcgct ggtatatttt catgctgcac atgccaggcg
     7261 acaggggcag aagtgacgag tgaatctgta tttaacattc ttaattccac gggggcagca
     7321 actgataaaa gttatttatc gttaaatcca gataagtatc ctaattatcg tcttctgatt
     7381 cactcagcca aattaaaaaa tgaaataaag agccactata ccaaagacga aattcaagga
     7441 ttgttaacat taacagaaaa tacacgaaag ttaacgttga cggagaaacc atggggaaca
     7501 tttattcttg cttctacttt cgaagatgac aaaacagcgg cagaaaccca ctatgatgct
     7561 gtctggctga gggatagttt atggggctat atggcattag tatcagatca ggaaaatagt
     7621 gtagccgcaa aaaaggttct gctcactctg tgggattata tgtctacgcc agaccagatt
     7681 aaacggatgc aggacgtcat tagcaaccca aaacgactgg atggtattcc tgggcaaatg
     7741 aatgctgtgc atatccgatt tgacagcaac tcccccgtga tggcagatgt gcaggaagaa
     7801 ggcaagccgc agctgtggaa ccataaacaa aatgatgcgt taggtcttta tcttgatctc
     7861 cttatacaag caatcgacac tggtactatt aacgctgaag attggcaaaa aggtgaccga
     7921 ttaaaatcgg ttgcgctgct gattgcttat ctggataaag ccaatttcta tgttatggaa
     7981 gattccggag cgtgggaaga agatgccaga ttaaacacat cttccgttgc actggtgacc
     8041 tctggactgg aacgtttgtc gaacttactc tcgaagaaag actctgtttt tgtctctgat
     8101 ttactacgcg aagctaaagc taatgaactg gatgaaccac tttcaacaac gaggttgaac
     8161 cacctcatag ataaaggtta tgaaagaata acgttacagc ttgatttagg gggagaatcc
     8221 ccaggttatc tggaaaaaga taaacattac agagaagccg atgcggcttt acttaatgtt
     8281 atttatcctg caaatctttc aaagataaat accagaagaa aagagcaagt tctaaaaata
     8341 gttaaaaaac tagcaggtcc gtatggtata aaaagatacg agaaggataa ttatcagtcg
     8401 gcaaattttt ggttcaatga tattaaaacc gataccgatc aaaattcaca tgcaaaaaga
     8461 gaaatgagct tcattccatc aacagaagca gaatggtttt ttgactcatg gtatgcaaaa
     8521 tcagcagcaa ttgtttataa agaatcaaga aaagaagagt atttgaatga ctcagtgcaa
     8581 tttatgaata gatcattggc ccaaataaca ggcgagaata tgattggtgc aaatggcagg
     8641 agcgtacctg aaatggcact tcccgaaagc tataactata tccacaagtc aggaactttg
     8701 catgaagcgc caagccctat cattccgcta aactggtcga aagcctcaat gactcttatg
     8761 ttgaaagaaa tgtccaatct tatcaatgat gagggaatta aatagttttc ataatgaagc
     8821 ggtcctggat taaaaggacc gctcaaagag aaatcgtaag ggaggtaaaa gtgatcaata
     8881 aattactgtt agcttacctg ataggtctgg ttgtgacatc gaccttaata tttatcttct
     8941 cggaagaaaa agtaacatat cggttgtttg ctgcagtaat tacagggttg acatggccgc
     9001 ttagcctcat tccatccatc ataagtctca tgattcgtaa atccgactaa caagaaaatt
     9061 atatgaacga ttaagaaaaa tagaatcgga aaagagaata aaatatatgc agggagtaac
     9121 aaaacactcg cagtgcgtta atgtgtaact aacaaatatg atttttattg aaaatagtca
     9181 ccaaacaagt gttataagaa atacggggta tgtttaagta aaacatatca acagttgcaa
     9241 ttctgtatat ggacaggatg acgtacaagt gtaagtggct gatagctatt cgctcataac
     9301 aaaatcatta ttttataaat aggaatcatc actttttaat catgttttta ttgcaagttt
     9361 atggagaatg agaatatgga ataaggagat gaaacatgat tgaagaaggg ctgttacttc
     9421 caatgaatgt tttgcttcaa ggacagaagt taaccctact ggaccctgaa atgtggttct
     9481 tacggggaaa tgaaaacaag gatgtaaccc ttgttattac gattggtaat aaccatcaaa
     9541 aattaatggt agtggaagac atattactgc tcattgaccg aagccaaatt gaggtaactg
     9601 cagggaaagt aatttatcat ccacttcgca ttgatatcct gagtaaatta ttagctttta
     9661 ttgatgaatc aacggggagt gtggaaaggg aacaccatga gttgtttgct gatgccttgc
     9721 cctttgcatc agaagttgtg ctattcagta aagttgcaag tgaagcctgg tttttagcca
     9781 cttatctgag cagcgataat attaatgaaa ttctgtttca gcatctaaga aaaacagaat
     9841 gctataaact ggttcgctat ttattatcgc aatcgctcat acagacttca ctctatgatc
     9901 ttggtgagct ttacggcgta tcgtattcac attttcgccg tttatgtagt tatgcattgg
     9961 gtggtaaagt taaaacggaa ttatgtggct ggagagttgc cagagccgtg ttggaaatca
    10021 ttgaaggaaa cagtgatatg acaactattg cgcataaata tggctactca tcctcatcac
    10081 acttttcagc tgaagtaaaa agtcggttag gaaaaacacc gagagaacta tgtaaaaagt
    10141 tatgaaaata aaattacgta ttactattat attaatttca gccttatgca tttttaatgg
    10201 attattgact cctggtgcat atgccgcaac agcgaatgga tacgtagcaa ataaagaaaa
    10261 tctacgtagt ttttttgaaa cggtttcatc atatgcgggt aagcctacaa tagtaagtaa
    10321 gcttgccatg aagaaacaga tcagcggaaa ttttgattta accgaaccct atgccttgat
    10381 tgaacgcctg tcggcgcaga tgggacttat ttggtacgat gatggcaaag ctatttatat
    10441 ctatgactca tcagagatgc gtaatgctct gattaatctg agaaaggtat cgactaatga
    10501 gtttaataat ttcttaaaaa aatcgggtct gtataactct cgttatgaaa ttaaaggggg
    10561 cggcaatggt actttctatg tatcggggcc accggtttat gttgacctgg tcgttaatgc
    10621 tgcaaaatta atggagcaaa acagtgatgg catcgagatc ggacgcaata aagtagggat
    10681 tattcacctg gtcaacacat tcgttaatga tcggacttat gaattacgtg gcgaaaagat
    10741 agttattcct ggcatggcaa aagtactatc gacgttgtta aataataaca ttaagcaaag
    10801 cactggggtt aacgtacttt ctgaaatttc aagtcgtcaa caactaaaaa acgtatcacg
    10861 tatgcctcct tttcctggtg ctgaagaaga tgatgatcta caagtagaga aaataatatc
    10921 gacagctgga gcgccagaga ctgacgatat acaaattatt gcatatcctg ataccaacag
    10981 tttacttgtt aaaggaacag tatctcaggt tgattttatc gagaagttag tagctacact
    11041 cgatattcca aagcgacaca ttgaattatc tttatggata atagatattg ataagactga
    11101 tctagaacag ttaggagctg actggtcggg cacgataaaa attggatcca gcctaagtgc
    11161 atcatttaat aattcaggtt caattagtac cctggatggg acacagttta ttgcaacaat
    11221 acaggcactt gcgcaaaaaa gaagagccgc cgttgttgct cgtcctgtcg ttttaacaca
    11281 ggagaatatt cctgctatat ttgataataa ccgtactttc tatactaaat tagtcggcga
    11341 gcgtactgcg gagttggatg aagtaaccta tggtacgatg attagcgtgc taccacgatt
    11401 tgcttcgcga aatcaaattg aattactctt gaatattgaa gatggcaatg aaatcaattc
    11461 tgacaagacc aatgttgatg atctgcctca ggttggtaga acgcttataa gtactattgc
    11521 gcgcgtgcct caagggaaaa gtcttttgat tggtggatat acacgagata cgaataccta
    11581 tgaaagtcgc aagatcccaa tattaggcag tattccattt attggtaaat tatttggata
    11641 tgaaggaaca aatgcgaata acatcgttcg tgtctttctt attgagccaa gagagatcga
    11701 tgagcgcatg atgaataatg cgaatgaggc tgcggttgac gccagagcaa taacacagca
    11761 aatggcaaaa aataaagaaa tcaacgatga attactgcag aaatggataa aaacttacct
    11821 gaatcgtgaa gtcgtcggag gatagtgaaa tggcgattca tgtagagcat gtcggtgtat
    11881 tagaaagagc cagagaagtt tctcgcttag aggacattat cacagaagat aatgaagata
    11941 ttgaagctga aatgcctaaa atgggggatg atcccgccgg taaagaagct cgattcttac
    12001 aagctactga cgagatgtca gcagcgttaa cgcagtttat gaagaaaaaa atctacgaag
    12061 aacaactggc caactttctg gacggagaag aatatgtcct ggaagatcag ccaatagaaa
    12121 aaaccgataa agtcatggag gcgcttaagg ctgcaactac ccatgactat gaagtgtata
    12181 gctttgctaa aaaattattt ccagatgaaa gcgatctggt ggttgtatta cgtgcaattt
    12241 tacgtaaaaa acaaatatct gaaaatgtgc ggctaaacgc tgaagcatta cttagaaaag
    12301 ttaatcaaga gactactaaa aaattcatta atagtggcat taactcggca ttaaaagcaa
    12361 agttatttgg ccaggcattg tcattaaatc caaaattact aagggcaagt tatcgacaat
    12421 ttttaatggc agaagacgat gctgtagata cttatgtaga atggattggt agttatggct
    12481 atcaaaatag aatgctagtg acaaaattta tcaaagagac gcttttctca gatataaatg
    12541 cacttgatgc gagttgttca tcattggaat ttggtatgtt tctcaataag ttgtcacaac
    12601 tattgtctct tcagtcggca gaggctttat ttctgaaaac tttaatgaat aacccaataa
    12661 ttaaaaaatt tattagcgca gaagattact ggatattttt tctgatatcg ttaataaagt
    12721 ttcctgagac tgcagaggaa ctcctgaaaa atgcactggt aaccctaccc gcagatgcta
    12781 attataaaga caaaacgtta ctcctgaagg cgatttacag cggatgtaca aatcttccat
    12841 tttcactttt tattaataat gaacaattat tagagattag ggagtgttgt aaacaggcaa
    12901 taaaagtaac atttgcagca gagctctttg atacacaaaa cagtaataaa aaacaaaata
    12961 aaaaaccatg gaagaaggta atgttcaatg tttaacaaag ttttgatagg gttaaggagt
    13021 catccagaat taataattct tggattaatg gtgatgatta tcgccatgct cataattcca
    13081 ttgcccacct acttaatcga ttttttgatt gggctgaact taacattggc aatacttgtg
    13141 tttttaggat cattctatgt tgataggatt ttaagttttt cttcattccc atcaattctt
    13201 ctcatcacaa cgttatttcg tttggcgctg gcgatcagta ccagccgact tatacttctt
    13261 gaggcggacg ccggagaaat tataacgagt tttggtgaat tcgttattgg agatagcctg
    13321 gtagttggtt ttgttatttt ctccattgtt actattgttc agtttatcgt gattactaaa
    13381 ggttcggaac gtgtggctga agttgctgcc cgcttttcgc ttgacggtat gccaggtaaa
    13441 caaatgagta ttgacgccga tctacgcgcc gggatcattg atgcagattt agcaaaagaa
    13501 cgtcgtagcg tattagaacg agagagtcag ctgtatggtt cctttgatgg tgcgatgaaa
    13561 tttatcaaag gtgatgctat tgcaaacatt attattatct ttgttaatat tatcggtggg
    13621 ctatcagtag gagtaggcca aaatggaatg gatttctcga cagcgttgac cgtctacaca
    13681 attcttaccg tcggtgatgg tcttgtttca caaattccag ctttattaat tgctattagt
    13741 gcagggttta tcgtaacgcg cgtgaatggc gatagtgata atatggggca aaatataatg
    13801 tcccagttat taagtaattc ttttgtcatt attgtgacat gtgtcctcgc gctaagtatt
    13861 gggttgcttc caggttttcc ccttcttgtg tttttatgtt tggcagtaat cttaggaatt
    13921 tatttttact ttaaattcaa aaagaaaggg gttgaagaaa cggttgttga gggagatatc
    13981 accgttgggt tggaacccta taatcaggat gatgatattt cattaggtat tattaataaa
    14041 cttgatcagg ttattaccga aacggtaccc ttggttttga ttatgaatag tgtacaggct
    14101 aaaaaataca ctgaaatcaa tcttgctgat aggattcgta gtcagttttt tattgagtat
    14161 ggtatccgta ttcccggtat tgtcattcgc gagggggaag gccttaatga tgaagatgtc
    14221 atattaatgc taaatgaagt cagagcctcg caatttaaaa tttatcatga tcttgtcttg
    14281 ttggttgaat attcagatga agtggtttcg actttgatca agaaacctgt catcgtaaat
    14341 aataatggtg aacaatatta ttgggttaca aaaagtgatg cccaaaaatt aacaaaaatt
    14401 ggctgttata ctcgcacggc catggatgaa atgtacaatc atttatctgt
//
LOCUS       DAYPHN010000126        14425 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000126 DAYPHN010000000
VERSION     DAYPHN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.126"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1052)
                     /gene="ccp"
                     /locus_tag="ACG0BW_003494"
     CDS             complement(<1..1052)
                     /gene="ccp"
                     /locus_tag="ACG0BW_003494"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFK7260418.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNQVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVELGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNK"
     gene            1457..3106
                     /locus_tag="ACG0BW_003495"
     CDS             1457..3106
                     /locus_tag="ACG0BW_003495"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFK7260419.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3157..3759)
                     /locus_tag="ACG0BW_003496"
     CDS             complement(3157..3759)
                     /locus_tag="ACG0BW_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFK7260420.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGAGQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4279..5178
                     /locus_tag="ACG0BW_003497"
     CDS             4279..5178
                     /locus_tag="ACG0BW_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7260421.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGASPEDGVIARPLGK
                     LTVVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLIYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5227..6240
                     /gene="yhjD"
                     /locus_tag="ACG0BW_003498"
     CDS             5227..6240
                     /gene="yhjD"
                     /locus_tag="ACG0BW_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFK7260422.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQR"
     gene            6632..7954
                     /locus_tag="ACG0BW_003499"
     CDS             6632..7954
                     /locus_tag="ACG0BW_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7260423.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8136..10196)
                     /locus_tag="ACG0BW_003500"
     CDS             complement(8136..10196)
                     /locus_tag="ACG0BW_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFK7260424.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVST
                     ELNRPFAIRGDLGVEWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLAGKGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     IQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINITGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10266..11033)
                     /gene="pdeH"
                     /locus_tag="ACG0BW_003501"
     CDS             complement(10266..11033)
                     /gene="pdeH"
                     /locus_tag="ACG0BW_003501"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFK7260425.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSYRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11265..12194
                     /gene="kdgK"
                     /locus_tag="ACG0BW_003502"
     CDS             11265..12194
                     /gene="kdgK"
                     /locus_tag="ACG0BW_003502"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFK7260426.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPE"
     gene            complement(12166..12750)
                     /locus_tag="ACG0BW_003503"
     CDS             complement(12166..12750)
                     /locus_tag="ACG0BW_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260427.1"
                     /translation="MRNKKRILFLLTVILCLTGLYSCNKNFLLLWDANNMYVSTRNNI
                     DKDKVKIEFGISVNTINRETDAELFADKDKYAVLFDGSLKNKMINEYGENDFLITYDN
                     RCYLSFRQFKTNRRHQHDYYFGFFNNNGNVFVTVEIKGENPLKFTRSLNDMRQQFTSN
                     YQHSSVHPCKAINGERISTRSPSKTIIPALPHAG"
     gene            complement(12812..13114)
                     /locus_tag="ACG0BW_003504"
     CDS             complement(12812..13114)
                     /locus_tag="ACG0BW_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260428.1"
                     /translation="MYRVKVVWLTENEGGRRSAPPIGRYYPVSRFPEEKTDWQNNAWS
                     VVFELEKPNVTDGRIVSMGCVQFLFDTAPEQWMTKYEAFEIYEGPRKVADVLLIGN"
     gene            complement(13136..13603)
                     /locus_tag="ACG0BW_003505"
     CDS             complement(13136..13603)
                     /locus_tag="ACG0BW_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260429.1"
                     /translation="MDILLPTRFAPEELDLQEVMVHWGGETFHRDPPVYAWCNHHLLQ
                     RCNLPITYGPPLDEHIDFNEYHVYNFNGSLVDDLEMAVNKGKDISTNPIIKFINNLVS
                     KNYGGWVILSLDDEKIEVIKNISFQYSFLSLLVDGLKWERSHGVAILYNSHLI"
     gene            complement(13715..13969)
                     /locus_tag="ACG0BW_003506"
     CDS             complement(13715..13969)
                     /locus_tag="ACG0BW_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260430.1"
                     /translation="MAYHIEILKIEETDKVVKYEFGPTSSDYCRSDYGEFVIDKRTGE
                     MTLVRMVKNDSANKFYLRASFKILTCWRKGYLPKNEEWAS"
     gene            complement(13969..>14425)
                     /locus_tag="ACG0BW_003507"
     CDS             complement(13969..>14425)
                     /locus_tag="ACG0BW_003507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260431.1"
                     /translation="IPVPTGRSEFMLVLKSQSIGFRISTTYLSLTYTESDGVNIKVDA
                     LELISTEKDYVQIELVFTTVAEVKCATVNFHEANYNNIVIKKVDGENLDSGIYQVMNS
                     VPLEEKERLYDPKKRLKLKHYIIAGYDSYVEILATGFSCIRCQAIDGVK"
BASE COUNT         3618 a         3727 c         3349 g         3731 t
ORIGIN      
        1 catttattat ctttaaataa ttgatagcct tttttctgtt gtgccgtcag cgcattctca
       61 tctccacgca accatttatc aaatggggaa tccggcgtaa ttaatgtttt ctcaaattca
      121 gcaatggcat cagtaatatt ttcgccactg aaaccttgcg gatagacttc gaggaactgc
      181 gctttaagct gcggatcttt ttccagctta gcaataattt cgtcccagga tttcgacgcc
      241 atttcaatcg gattcaacgg cggtccacca gcctgatcct gcaatgttgc cgcacgacca
      301 tcccagaact gctcaacgtt aaatactgag ttaaataccg tcggcgcgtt aatcgggccc
      361 actgcgccac caacaccaat cgatgttttt ctgccatcga cgccccccgc attcaacgca
      421 tggcaatgag cgcatgaaat ggtgctatca gccgataaac ggggatcgtg atacagcgca
      481 aaacccaact ccactttttg cgcatcggta ggcagttttt gcgggatggg ctgcaccggt
      541 tcattgcgat gctccggagc agtatcattg ctggcgtaat attccgcgcg ctgttttgca
      601 atccaggcca gtatttccgc ccgctcttca tcactcacct tacccgccca gtgtagcgcg
      661 gtataacgcg ttggcggcat agtttcatac tgcatcaccc attcaatctt attcagatcg
      721 ctttgcgaaa cgggtttatc agccaacaga gccgcacgca cggcctcgag gttaaaagat
      781 ttatatccaa gctttatgtc gtaatccatc aactgtttcg cgccgggaat ataataatag
      841 gcaggtaatt ctgccgaagg cgtatggcaa taatcgcatc ctttttcgcg gagaaagcct
      901 aaaacctgat tattttcact gacagcagat gcctgaacat cggcctgttt actgcgttta
      961 ttatcgtggt accacacata accagataac cctaaatagc aaatcgcgac gccagccagg
     1021 ccgatcgcgg taatacgtga gaccattttc attattcttc cctcacggtg gtgagttatt
     1081 gtgacccaga aaattttcca gaatgctgca aacgtctctg cgagacaaag ccatcataaa
     1141 aaacagggag atacgctttt tgataggaat caatcaatta catgaatata aactatcaat
     1201 aagatagcct gtgcctatta cccagatcag aaatggcata tcatgattgc ggcgagagca
     1261 ggattgaatg ttctgaaaat gaaaaacaaa accaaatccg tatttttata aattccattg
     1321 atattagtgc gttatatttt ttgacaagtt aataaacgtg atctaccgca cgctttgtcg
     1381 cccaccaggc ggagcgaatg actaccctta aagaaaagcc cgataattag cgacgaattt
     1441 cggaggttgg atccttatgc tcaatcagaa aattcaaaac cctaatccag acgaactgat
     1501 gatcgaggtc gatctctgct atgagctgga cccgtatgaa ttaaaactgg atgagatgat
     1561 cgaggcagaa ccggaacccg agatgattga agggctgccc gcctctgatg cgctgacgcc
     1621 tgccgatcgc tatctcgaac tgttcgagca tgttcagtcg gcgaaaattt tccccgacag
     1681 taaaaccttt cccgactgcg cacctaaaat ggacccgctg gatattttaa tccgctaccg
     1741 taaagtgcgc cgtcatcgtg attttgactt gcgcaagttt gttgaaaacc acttctggct
     1801 accggaggtc tactccagcg agtatgtatc ggacccgcaa aattccctga aagagcatat
     1861 cgaccagctg tggccggtgc taacccgcga accacaggat cacattccgt ggtcttctct
     1921 gctggcgctg ccgcagtcat atattgtccc gggcggccgt tttagcgaaa cctactattg
     1981 ggattcctat ttcaccatgc tggggctggc ggaaagtggt cgggaagatt tgctgaaatg
     2041 catggccgat aacttcgcct ggatgatcga aaactacggt cacatcccca acggcaaccg
     2101 cacctattat ttgagccgct cgcaaccacc ggtttttgcg ctgatggtgg agttgtttga
     2161 agaagatggt gtacgcggtg cgcgccgcta tctcgaccac ctgaaaatgg aatatgcctt
     2221 ctggatggac ggtgcagaat cgttgatccc taatcaggcc tatcgccatg ttgtgcggat
     2281 gccggacgga tcgctgctca accgttactg ggacgatcgc gacacaccgc gtgacgaatc
     2341 ctggcttgag gacgttgaaa ccgcgaaaca ttctggtcgc ccgcccaacg aggtgtaccg
     2401 cgatttacgc gcgggggcgg cctccggttg ggattactct tcccgctggc tgcgtgatac
     2461 tggtcgtctg gcgagcattc gtaccaccca gttcatcccc atcgatctga atgccttcct
     2521 gtttaaactg gagagcgcca tcgccaacat ctcggcgctg aaaggcgaga aagagacaga
     2581 agcgctgttc cgccagaaag ccagtgcccg tcgcgatgcg gtaaaccgtt acctctggga
     2641 tgatgaaaac ggtatctacc gcgattacga ctggcgacgc gaacaactgg cgctgttttc
     2701 cgctgccgcc attgtgccgc tctatgtcgg tatggcgaac catgaacagg ccgatcgtct
     2761 ggcaaacgcc gtgcgcagtc ggttactgac acctggcggg attctggcaa gcgagtacga
     2821 aaccggtgaa cagtgggata aacccaacgg ctgggcaccg ttacaatgga tggcgattca
     2881 gggatttaaa atgtatggcg atgaccttct gggtgatgaa atcgcgcgca gctggctgaa
     2941 gacggtgaat cagttctatc tggaacagca caaactgatc gagaaatacc atattgccga
     3001 tggtgttccc cgcgaaggcg gcggtggcga gtatccgttg caggatgggt ttggctggac
     3061 taacggtgtg gtacgccgtt taattggttt gtacggcgaa ccataatatt tttacagcca
     3121 gccgctaact tcctgctggc tgtaaaatta tcctcttcag gaggagatat ttaacatcat
     3181 tgccgcctgg gtgcgatttt tcacttccag acggcgatac agggattcca gatgcgcttt
     3241 taccgttccg gtactgatat tcagcgccct gccgatctct ttatttgatt cgcccgccgc
     3301 taacatggtt aaaatctccc gctggcgggc gcttaacgat ttgagatctt taatgtcctt
     3361 ttccggagtc gtccgccagt ctccaggcag aaacatcatc cccatcgccg cactatttac
     3421 cgccaacgca aatgtctcga cggttgaatc acgaggcaca atggccagca cattaaaatg
     3481 gataacttcc tgtaaccacc gtttattaca atccgtcgcc gtaattaaca ccttaacctc
     3541 aggaaattgc accacggttt tttgcagcaa ccagtagcaa aactcaccat cctgatcgcc
     3601 atcgagcatc actaaggctt cagggtaact ttccagcttt tgccataact cgtctgcctg
     3661 accggccccc tgaatactca ctcctggaat acgctgctgt aaactgattt tcattccatg
     3721 aataaatatt gactgcctgt caaacatgac tatttgcata actgaatctc cacctgaata
     3781 cgttaaaaag acttaagtag tggaagggta ttacccgcga gaaaaaataa gaattcgcca
     3841 tttggcggtg gccattctac agagatgacg cgtagaaaat agttaccgat ataaatagtt
     3901 acagctaaac gcctgaaatt acatgtcgag ggcactattt aaaacaattt tgaggatttc
     3961 cttatattga tggttagtac gcatgcaatt aaaaatgaaa ctccgcgacc acaagcaaaa
     4021 ataacaaacg gcaatgagac aaaaataagc acaaatagcc aacacgtcct ctgttcactt
     4081 taaagggaat cgctgaaaaa aacgctctgt ttagggaaat tcacctttct cataaagcta
     4141 ttccgccctt ttcctgctga gaaatcgcca cattcgtcat gacaacattg tgaaacccgg
     4201 cataagatgt tagaaaaaac aataaacaat gcgatatgcg cgctactgtt tcgctgtgaa
     4261 caacaatcgg tcaaagaaat ggataaaatt cacgcaatgc agttgttcat caaagtcgct
     4321 gagctggaaa gtttttcccg cgcagcggat ttctttgctt tgccaaaggg aagtgtttcg
     4381 cgccagatac aggcactgga acatcaactt ggcacccagc ttctccagcg caccacgcgg
     4441 cgggtcaaac tcacgccaga aggcatgacc tattatcaac gggcaaaaga tgtgttgagt
     4501 aatctcaacg aactggacgg tctgtttcaa caggatgcca ccagtatcag cggtaaatta
     4561 cgcatcgaca tcccgccagg aatcgcgaaa agcctgttac tgccgcgtct gtcggaattt
     4621 ctctatctgc atccgggaat tgagctggag ctgagtagcc atgaccgtcc ggtagatatt
     4681 cttcacgatg gttttgattg cgtgatacgc actggcgcgt caccggaaga tggcgttatc
     4741 gcccgtcccc tcggcaaact gaccgtggtc aattgtgcca gtccacacta tctgacgcgc
     4801 tttggttatc ctcaaagccc cgacgatctg acttcgcacg caatagtgcg ttacacaccg
     4861 cacctgggtg tacatccgtt aggttttgag gttgccagcg ttaatggcgt ccagtggttt
     4921 aagtctggcg gcatgttgac ggtaaacagt cgcgaaaact atctcgccgc cggtcttgcc
     4981 ggtctgggga ttattcagat cccgcgcatt gccgtgcgcg aagccctgcg tgccgggcgg
     5041 cttattgaag tgttgcctgg ctaccgtgcc gagccgctct ccctttcgct gatttatccg
     5101 cagcgtcggg agctttcccg gcgtgtaaac ctgtttatgc agtggctggc tggcgtaatg
     5161 aaagagtacc tggactgacc gactatactt tttaagaatg accacgacag aaggacaaaa
     5221 gagcggatga cgcaggaaaa cgagatcaaa cgtcccaccc aggatctgga gcacgagccg
     5281 attaagcagt tggataatag cgagaaaggc ggcaaagtta gccaggcgct ggaaaccgtc
     5341 accaccactg ccgaaaaagt ccaacgccaa ccggttattg cgcacctgat tcgcgcaaca
     5401 gaacgcttta acgatcggct gggtaaccag tttggtgcgg ctatcaccta tttctcattt
     5461 ttatcgatga tcccgatttt gatggtgtcg tttgccgcag ggggctttgt gctggcctcc
     5521 catccgatgt tgctacagga tatcttcgac aaaattctgc aaaacatcag cgatccgacg
     5581 ctagccgcca cgttgaaaaa caccatcaac accgccgttc agcagcgtac taccgtaggg
     5641 cttgtcggcc tggcggtggc gctttattcc ggcatcaact ggatgggtaa cctgcgtgaa
     5701 gcgattcgcg cccagtcgcg cgatgtctgg gaacgctcgc cgcaagatca ggagaagttc
     5761 tgggtaaaat atctgcgtga ttttatttcg ctgattggtt tgttgattgc actgattgtg
     5821 acgctttcga tcacctcggt tgccggttcg gcgcagcaaa tgattattag cgccctgcac
     5881 ctgaacagca ttgagtggct gaaaccgacg tggcggttga ttggcctggc gatttccatc
     5941 ttcgccaact atctgctttt cttctggatc ttctggcgct tgccgcgcca ccgtccgcgc
     6001 aaaaaagccc tgattcgcgg aacatttctg gctgcgattg gttttgaagt gattaaaatc
     6061 gtgatgacct acaccctgcc atcgctgatg aaatccccct ctggcgcagc atttggttcc
     6121 gtgctgggac tgatggcgtt tttctacttc ttcgcccgtt tgacgctgtt ttgcgcggcg
     6181 tggattgcca ccgccgaata taaagatgac ccgcgaatgc cggggaaaac gcagcggtaa
     6241 ttcaatggtt tataatgcct gataaacgca cggtcgatcc cctcgcccct tcggggagag
     6301 gattagggtg agggggtaca agccagccag agaccaggca atgacatgca cataaccacc
     6361 tgaaactaca tttcttcagg agcaaacaat ttcataccaa ctcataaccc cagcatataa
     6421 atccagttgg taacttttat ttaacctgaa accagtttta tccactattt ataaaattat
     6481 gtgaagcatt tcatagaaga aaaatcactg gcctaaacat tatccccttt ttgcctggtt
     6541 tttgaccatt tccgcgattt gttacacatt gaaatatcac ttttgctgtg cgtaatatgg
     6601 ctattcgtta gccaaaaaat aagaaaagat tatgcaagca acagccacaa cactcgacca
     6661 cgagcaagaa tacacgccga tcaactcgcg taataaagtc cttgtcgcct ctctcattgg
     6721 cacggccatt gagtttttcg acttttacat ttacgccact gcggccgtta ttgtgtttcc
     6781 gcatatcttc ttcccacagg gcgatcctac ggcagcaacg ctccagtcgc tcgccacctt
     6841 cgccatcgcc ttcgtcgcgc gccccattgg ctctgccgtt tttggtcatt ttggcgatcg
     6901 cgttgggcgt aaagcgacgc tggtcgcctc gttgctaacg atggggattt cgaccgtggt
     6961 gattggtctg ctgccgggct atgccacgat tggtattttc gccccgctgc tgctggcgct
     7021 ggctcgattt ggtcagggtc tgggcttagg cggtgaatgg ggcggcgcgg cgctgctggc
     7081 gactgaaaac gccccaccgc gcaaacgtgc actgtatggc tccttcccgc agctgggcgc
     7141 accgattggc ttcttctttg ccaacggcac tttcttgctg ctttcctggc tactgaccga
     7201 cgagcagttt atgagctggg gctggcgtgt gccatttatc ttctcagcgg tgctggtcat
     7261 tatcggcctg tatgttcgcg tgtcgctgca tgagtcgccg gtgtttgaga aagtcgctaa
     7321 agcgaaaaag caggtgaaga tcccgctggg tacgctgctg accaaacacg tacgcgtcac
     7381 ggtgctcggt accttcatca tgctggcaac ctatacgctg ttttacatca tgacggtcta
     7441 ctctatgacc tttagtaccg ccgccgcgcc agttgggctt ggcctgccgc gtaacgaagt
     7501 gttgtggatg ctgatgatgg cagttattgg ttttggcgtg atggtgccag tcgccggatt
     7561 actggctgat gcctttggtc gccgtaaaag catggtaatc atcaccacgc tgatcatcct
     7621 gttcgcgctg ttcgccttta acccgctgct cggttctggc aacccgattc tggtttttgc
     7681 cttcctgctg ctggggttaa gtctgatggg actgaccttc gggccgatgg gcgcgctgtt
     7741 accagagctg ttcccgacag aagtgcgtta caccggagca tcgttctctt acaacgtagc
     7801 gtcgattctc ggggcttccg ttgcgccgta tatcgcagcc tggttgcaga ctaactacgg
     7861 gttaggtgcg gtggggttat atctggcggc gatggctggc ttgaccttaa tcgccctact
     7921 gctgacccat gagacgcgac atcaatcgtt gtaataagag tgaaaagcct gatgcgctac
     7981 gcttatcagg cctgaatttc catacaatat attgaattcg caaagatttg taggacggat
     8041 aaggcgttca cgccgcatcc ggcatgaaca aagcacactt tgccaacact ctaaatctgt
     8101 ttaattgccg gatgtcagac atccggcaat tatcatcact tcttcatctg cgacaatatc
     8161 gtccgacact gattagcctc cccttcggaa ggtgagatca gcgccagtaa cgccgccgct
     8221 ggcgtaacca gcgttgccag tgccgccgca acggctccgc gagcaatcaa cggcccggct
     8281 ttcaccccag cctgcggatt tttaaacgtc ccacgcacat acagcggcga acgcagtgtg
     8341 ataatccgaa ttcctttact ctccggatca atagtcaaat ccagctgttc cgaagcaaaa
     8401 cttgccgtgc cggtaatgtt aatcaacgcg ttctcagtat cgaaagcaaa aatctgcgga
     8461 cgcgccacgc cgttggcaat atccagattc gccgccgcac agttcacccg cacctcatcg
     8521 tcaccaaata tcgcaccgac aatgtagtta ccgacattca gcccgacaat ctccatcagg
     8581 ttgcggctca ccagcccgtc attcatcagc agtttcaggt tgccgttact gttgcctaaa
     8641 agcgccgcca ccgagttacc gctaccgcgt agttccgcgt caccgttcat ttcccccagc
     8701 gtcttctgca tcagttctac atcgggcatc agttctttca gtttcagccg acgagcctga
     8761 atatccgccc gcccctgcat cggtttttta tcgccctcca gatgaatatt cgccgcaatg
     8821 ctgccgcccg ccatgccaaa tttcagcggt tgcaggcgca ggtcagcatt tttgaggatg
     8881 atatgagtag aaagatcgct aatcggcagg ctactgccat gctcaatgcg ccgccctttg
     8941 aagcgaacat cggcatccat aacgtcccat ttatcggttt cgaagcggtc ataaggcagc
     9001 actttgcccg caggctgaat gcttttttca cccttcttct gttcagaccg tttcgacttt
     9061 tcagctcctt tcccggaatc aacgccaatc aacggtccca ggtccgccag ccgcaattgc
     9121 cgcgactcga catcaccttc tagttttggt cgtggcttgc cggtggtgta gaccagagaa
     9181 ccgtggatat cgctatcgcc aatgcgccca ttaaaaccgc gataatcaaa gaccgacgat
     9241 ttttcagtgt cgattttcgc taccagccga ccatccgttt caaacggcgg ggtatcgggc
     9301 agcagaacgc ccgtcagttc atagagatca cccagcgaat cgccagaaaa tttaagccgt
     9361 aaatcgacac cacccatctt cattgggtca ttcacaacgc catcaaaagc aacacgggtg
     9421 ttaccagagc ggaaatcagc ctgtaccgga aacggcgtcc cttcgccacg cagcgccagc
     9481 ataccgccaa ttttcccctt acccgcgagc ggctctccgt tataacgtcc ctgcgccttc
     9541 aggccaaaaa cgtaatcgcc caccttttct ttatccgctt tacctttcga tccagtaact
     9601 tcgctgaacg gcagcggctt gcctaacgga tcgacaaaaa tttccagatc cgctttgctt
     9661 actttgtcat caatggcgat ccgcccttga tcgaacagaa tattatccag ccgaaacgac
     9721 catgccgacg gctttgcatt cgtgtctttg ttgtcatcgt tggcgagatt aaaggtccag
     9781 ttattgttct tttcagagag gcgaatcagg cgtgcgtcgg gcttttccag tttgatccac
     9841 ggtagccaga ccgttttggt cagcagcgcc aacggtgcca gcgttgcctc tacgcgtggc
     9901 aaatgcacca tcgtgacttc gggaatatcc ggtggattgc caagaatgat gtcttccgcg
     9961 tgtacatggg gccacggcac ccagctgcgc cagccagttt cttgtttttg tcgctcccac
    10021 tccacgccca gatcgccacg gatagcgaac ggacgattca gttcagtaga gactttctgg
    10081 ttgatggtcg gtttaaggcg attccagtca aaagttgcaa tcaaaatgat cgccacgaca
    10141 atcaacaaca agaaagcccc tgaaatcgca gcggttattt tgcctgcctt gctcatcgtt
    10201 cgcttcctca tattcttcct gtgccagtcc taaagatagt ccagccaggc ggaaaatgag
    10261 gcagcttata gcgccagaac cgccgtattc agcgtttcta tcggtgccgg gcgtgaaaga
    10321 aaccagcctt gtgcggcgaa tgcgggcgag ttctgaacat cacgccactc ttccggcgtt
    10381 tctacgccct cgacgatcac cccgcgacaa tagcgattca tgagatgtaa aagctgagaa
    10441 aagagtgtgc gtccttccgg cgactgacgc agcatcacaa acagctctcg cgcgattttg
    10501 atgtagtcat aacgcacttc acttagcgca gagaaatttg ccatcccggt gccaaaatca
    10561 tccagccaca gcgggccaaa ttcacacatc gaggcaaagg ttgaatcttt cggcagacgg
    10621 atgtgttcca ccagttcgaa acgcagccag ggaagacgct caatctggcg caggattttt
    10681 ggttgctgac gcagggcgat gagcgtaggg ccatcaatat tgaccgatgc cagcaggtcg
    10741 tgctctacaa agaagtcggc tttttgcgcc agcaaatcaa tctgctcttt cacaacctcc
    10801 atccgatagc tgacggtgat ttcagtaaaa tagcgatccg gcggcaggcg ttgcgaaggg
    10861 ttcaagggat gcgtgaccac cgttaatagc tccacggcca ttaaccgccc gcatgtttga
    10921 tagatcggct gccaggtgta agcacgctca cactgcaacc aaaaacgccg ttcctgcaag
    10981 ctctcgatac ttgcttcagg gttgcttatt cgctggataa cctgccttat catcaaagat
    11041 gtcctgtgtt taaaagtgat gctgcccgga ctcgacaaag attatcggcg cgcaagggca
    11101 gaactttacc gcctggccgg gttaaatttc cccactccac aacggaatct ttcaggaata
    11161 cgttctggct caaaaaaata atggaacact gttttaatat ggttgaccag caaaccacca
    11221 cagcgcaaac taacgctaat tttttacaga tcaggttcac gactatgtcc aaaaagattg
    11281 ccgtgattgg cgaatgcatg attgagcttt ccgagaaagg cgcggacgtt aagcgcggtt
    11341 tcggcggcga taccctgaac acctccgtct atatcgcccg tcaggtcgat cctgcggcat
    11401 taaccgttca ttacgtaacg gcgctgggaa cggacagttt tagccagcaa atgctggacg
    11461 cctggcacgg cgagaacgtt gatacttccc tgacccaacg gatggaaaac cgtcttccgg
    11521 gcctttacta cattgaaacc gacagcaccg gcgaacgtac gttctactac tggcgtaacg
    11581 aagccgccgc caaattctgg ctggagagcg agcagtctgc ggcgatttgc gaagagctgg
    11641 cgaatttcga ttatctctac ctgagcggga ttagcctggc gatcttaagc ccgaccagcc
    11701 gcgagaaact gctttctttg ttgcgcgaat gccgcgccaa cggcggaaaa gtaattttcg
    11761 acaataacta tcgtccgcgc ctgtgggcca gcaaagaaga gacacagcag gtgtaccagc
    11821 aaatgctgga atgcacggat atcgccttcc tgacgctgga cgacgaagac gcgctatggg
    11881 gtcaacagcc ggtggaagac gtcattgcgc gcacccataa cgcgggcgtg aaagaagtgg
    11941 tggtgaaacg cggggcggat tcttgcctgg tgtccattgc tggcgaaggg ttagtggatg
    12001 ttccggcggt gaaactgccg aaagaaaaag tgatcgatac caccgcagct ggcgactctt
    12061 tcagtgccgg ttatctggcg gtacgtttga cgggcggcag tgcggaaaac gcggcgaaac
    12121 gcgggcacct gaccgcaagt accgttattc agtatcgcgg cgcgattatc ccgcgtgagg
    12181 caatgccgga ataatggttt ttgacggaga acgggtagat attcgttctc cgttaatcgc
    12241 cttgcagggg tgaaccgaac tatgctgata atttgaagta aactgctgac gcatatcatt
    12301 caaactacgc gtaaatttaa gcgggttctc acctttaatt tcgaccgtga cgaagacatt
    12361 cccattgttg ttaaagaagc caaaataata gtcatgttga tgacgacgat tggttttaaa
    12421 ttgacgaaat gataaatagc agcggttatc gtaggtaatt aaaaaatcat tttctccgta
    12481 ttcgtttatc atcttatttt tcaggctacc atcgaagagc actgcatact tatctttgtc
    12541 agcaaataat tcggcatcag tttctcggtt gatcgtgttg acgctgatac cgaattctat
    12601 ttttaccttg tctttatcga tattatttct tgtgctgaca tacatattat tggcatccca
    12661 gagtaagaga aagtttttgt tgcaggaata aagacctgtt aagcaaagaa tgactgttag
    12721 caagaatagt atgcgttttt tgttacgcat ttatactcct gaaaatgcgt tgaggtcgca
    12781 aatataatta aatagtctga cataaataaa attaattccc tatcaataat acatctgcga
    12841 cttttcttgg tccttcgtag atctcaaatg cctcgtactt agtcatccat tgttcagggg
    12901 cggtatcgaa aaggaactga acgcacccca ttgaaacgat acgtccatcg gttacattag
    12961 gtttttccaa ttcaaaaaca acgctccatg cattattttg ccagtcagtt ttctcctctg
    13021 gaaacctgga gacgggataa tatctaccga taggaggtgc gctccttctg ccaccttcat
    13081 tttctgtaag ccaaacaacc tttacacgat acatatgttt ccattcattc attaattaaa
    13141 ttaagtgtga attatataat attgccaccc catgcgatct ttcccatttt agtccatcaa
    13201 ctaataatga tagaaaagaa tactggaagc ttatattttt aatcacttct attttttcat
    13261 catctaatga caagattacc caccccccgt aatttttgct aaccaggttg ttaataaatt
    13321 taattatagg attggttgaa atatcctttc ctttattcac agccatttcc aggtcatcga
    13381 ctaaagaacc attgaagttg taaacatgat attcattaaa gtctatgtgc tcatctaaag
    13441 gtgggccata agtaatcggt agattgcatc tctgcaaaag atgatgatta caccaggcgt
    13501 ataccggagg gtcgcgatga aaggtttccc caccccagtg aaccataacc tcttgtagat
    13561 ctagttcctc tggagcgaat cgtgtaggga gtaaaatatc caagtgaaaa tccatagcct
    13621 atcctaaatt tggcacccat aacataaact gtgtaagaat caacgactgc ccccccaaaa
    13681 atgggaaaat ttacttatta tgcaaagtta cttattaaga agcccattcc tcattttttg
    13741 gcaaatagcc ctttcgccaa caggtcagaa tcttaaaact cgctcgcaaa taaaatttat
    13801 tagccgaatc gtttttaacc attctgacta atgtcatctc acccgttctt ttatctatca
    13861 cgaactcgcc gtaatcagat ctacaatagt cagaagatgt aggtccaaat tcatatttta
    13921 ctactttatc agtttcttca attttcaata tttctatatg ataggccatt atttaacacc
    13981 atctattgcc tggcatcgta tacagctaaa accagtagct aaaatctcaa cataactatc
    14041 ataacctgct ataatataat gttttaactt taaccgtttt ttagggtcat ataatctctc
    14101 tttttcttca agcggtacag aattcattac ttgatatata ccggagtcca gattttcacc
    14161 atcaaccttc ttaataacaa tattgttata gttggcttca tgaaaattga cggtagcaca
    14221 cttaacttca gcaactgtag tgaagaccag ttcaatttga acataatctt tctctgttga
    14281 tattaactcc agagcatcta ctttaatgtt gacgccatca gactctgtat atgttaaaga
    14341 taaataagtt gtggaaatcc tgaatccaat cgattgtgat tttaacacaa gcataaactc
    14401 actccttcca gtaggcacag gtata
//
LOCUS       DAYPHN010000127        14296 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000127 DAYPHN010000000
VERSION     DAYPHN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.127"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(135..1469)
                     /gene="srmB"
                     /locus_tag="ACG0BW_003508"
     CDS             complement(135..1469)
                     /gene="srmB"
                     /locus_tag="ACG0BW_003508"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFK7260432.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            1601..2338
                     /gene="trmN"
                     /locus_tag="ACG0BW_003509"
     CDS             1601..2338
                     /gene="trmN"
                     /locus_tag="ACG0BW_003509"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFK7260433.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(2323..3945)
                     /gene="nadB"
                     /locus_tag="ACG0BW_003510"
     CDS             complement(2323..3945)
                     /gene="nadB"
                     /locus_tag="ACG0BW_003510"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFK7260434.1"
                     /translation="MNTLPDHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGGGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            4201..4356
                     /gene="rseD"
                     /locus_tag="ACG0BW_003511"
     CDS             4201..4356
                     /gene="rseD"
                     /locus_tag="ACG0BW_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFK7260435.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            4353..4928
                     /gene="rpoE"
                     /locus_tag="ACG0BW_003512"
     CDS             4353..4928
                     /gene="rpoE"
                     /locus_tag="ACG0BW_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFK7260436.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            4961..5611
                     /gene="rseA"
                     /locus_tag="ACG0BW_003513"
     CDS             4961..5611
                     /gene="rseA"
                     /locus_tag="ACG0BW_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFK7260437.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            5611..6567
                     /gene="rseB"
                     /locus_tag="ACG0BW_003514"
     CDS             5611..6567
                     /gene="rseB"
                     /locus_tag="ACG0BW_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFK7260438.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            6564..7043
                     /gene="rseC"
                     /locus_tag="ACG0BW_003515"
     CDS             6564..7043
                     /gene="rseC"
                     /locus_tag="ACG0BW_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFK7260439.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            7241..9040
                     /gene="lepA"
                     /locus_tag="ACG0BW_003516"
     CDS             7241..9040
                     /gene="lepA"
                     /locus_tag="ACG0BW_003516"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFK7260440.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            9056..10030
                     /gene="lepB"
                     /locus_tag="ACG0BW_003517"
     CDS             9056..10030
                     /gene="lepB"
                     /locus_tag="ACG0BW_003517"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFK7260441.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            10303..10983
                     /gene="rnc"
                     /locus_tag="ACG0BW_003518"
     CDS             10303..10983
                     /gene="rnc"
                     /locus_tag="ACG0BW_003518"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFK7260442.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            10980..11885
                     /gene="era"
                     /locus_tag="ACG0BW_003519"
     CDS             10980..11885
                     /gene="era"
                     /locus_tag="ACG0BW_003519"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFK7260443.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            11897..12625
                     /gene="recO"
                     /locus_tag="ACG0BW_003520"
     CDS             11897..12625
                     /gene="recO"
                     /locus_tag="ACG0BW_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFK7260444.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            12637..13368
                     /gene="pdxJ"
                     /locus_tag="ACG0BW_003521"
     CDS             12637..13368
                     /gene="pdxJ"
                     /locus_tag="ACG0BW_003521"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFK7260445.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            13368..13748
                     /gene="acpS"
                     /locus_tag="ACG0BW_003522"
     CDS             13368..13748
                     /gene="acpS"
                     /locus_tag="ACG0BW_003522"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFK7260446.1"
                     /translation="MAILGLGTDIVEIARIESVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            14168..14248
                     /gene="shoB"
                     /locus_tag="ACG0BW_003523"
     CDS             14168..14248
                     /gene="shoB"
                     /locus_tag="ACG0BW_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFK7260447.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         3478 a         3546 c         3824 g         3448 t
ORIGIN      
        1 acgccttatc cggcctacaa gatcgtgcaa attcaatata ttgcatgagc catgtaggcc
       61 tgataagcgt agcgcatcag gcagttttgc gtttgccagc acttttaatt ccgggcttaa
      121 acccggaatt aagattactc ttctgtcgtt tgcggtggca cgcccgttcc gcttggttta
      181 cggcgcttac caatattttt ggtatcgcga tggcgttttt tcactcgcgg cttctctttc
      241 tctttagctt ttttcttctc ggcgcgttta gccagcactt tcttcgatgg cttgccggtc
      301 tgcttctcgc ttggtgcacg cgttttcggg cgtaactcat caattacgcg agctttaatt
      361 ggctcttcaa tataacggcc aactttgcct agcagcagat ggtcatgggc ttccactagc
      421 gaaattgcgg tgcctttacg accggcgcgc gcggtacgtc cgatacggtg caaataagta
      481 tcaccactac gcggcatatc gaagttaaag acgtggctga catcaggaat gtcgataccg
      541 cgtgcagcaa catcggtggc aaccagtacg tttacgcggc cttcggtcaa acgcttgatc
      601 gcttcgttac gcttgccttg taccatctca ccttcgagat agcagttgtt gatgcccgcc
      661 tcgcgcagcc agtttgccag ctcatgcaca cgctcacgct tacgcacaaa cacaattgag
      721 cgagtcgctt caggctgttt taacaggtgc accagcaacg cggttttatg ctcaagatca
      781 tcggcgcggt aataccactg atggatcttt ttgcgctcac gggtggaggg attcgcagaa
      841 acttccaccg gatcttccag cagacgctcg gcaaagtcct gaatcgcatc gccttccagc
      901 gttgcggaaa agagcagggt ctgtttacgc cagcgcgttt cgccagcaat atgttcgata
      961 tcctgagcga agcccatatc cagcatacgg tctgcttcgt cgaggataag agtttcaacc
     1021 gcgcggcaat cgaagttctc ttcttttatg tattgcagca aacgtccggt cgtggcgacc
     1081 acgatgtcct gattttcgct gaacacttcc gcgtggttca tataggctac gccgccggtg
     1141 atggtggcga tatccagatg cgtatgtttc gccagttcgc gggcatgatc ggccacctgc
     1201 atcgccagct cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga tttcttacgt
     1261 gggaaatcga gcaggtgctg caacgctggc agcagatacg ccgccgtttt accggtgcct
     1321 gtcggcgcag aaccgagtac atcacggcca tcgagcgcag gcggaatggc ggcagcctga
     1381 atggcggtcg ggcgagtgaa acctttatcc tggagggctt ccagcaggct ttcgtcgagt
     1441 tcaagttcgg aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg ctgattatag
     1501 acgttacggc tgcaatcttc atctgtttgt atggatatcg cttctcgggt attgttctca
     1561 cccggtgcta tcgccgtttt tcctgcaagg ttttattttc atgtcacagt ctacatccgt
     1621 gcttcgtcgt aatggattta cttttaaaca gttttttgtt gctcacgatc gttgtgcgat
     1681 gaaagtggga acggatggca ttttgctggg cgcatgggca ccggtggctg gggtaaaacg
     1741 ttgccttgat atcggcgcgg gtagcgggtt gctggcatta atgctggcgc agcgaaccga
     1801 tgacagcgta atgattgatg cagttgaact ggaaagtgaa gctgccgcgc aggcgcagga
     1861 aaatatcaac cagtccccgt gggcagagcg gattaacgtc catacggcgg atattcagca
     1921 gtggatcaca cagcagacag tacgcttcga tttaatcatc agtaacccac cttactatca
     1981 gcagggagta gagtgctcga cacctcaacg ggaacaggct cgctatacca ctacgcttga
     2041 tcacccatcg ttgctgacct gtgcggcgga gtgcattacc gaagagggat ttttctgcgt
     2101 ggtgctgcca gagcagattg gtaatggttt tactgaactg gcattaagta tgggctggca
     2161 tttacgtttg cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct
     2221 ggcattctcc ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc gtgggccaga
     2281 ccagaactat tccgaagcgt atacggcgct gacccaggct ttttatctgt ttatgtaatg
     2341 attgccgggg gaaaggatcg acggaccgga atgggtgagc agttccggat aatccagcgt
     2401 gaaatgcaac ccccgactct ctttacgcat cattgcacag cgaacaatca actcggcaac
     2461 ctgtaccaga ttacgcagct ccagcaaatt atttgagacg cggaaatggg cgtaatattc
     2521 gtctatttct tgttggagca tggttatccg ccgcagggcg cgttcaaggc gcttcgttgt
     2581 gcgcacaatg ccgacgtaat cccacataaa caggcgtagc tcgtgccagt tatgctgaat
     2641 tactacccgt tcgtcagggt tctcaacgcg gctttcatcc cacggtggta acgtactgac
     2701 gccgtgggca taaggcatac gtctggtgat atcttctgcc gccgaccagc catagaccag
     2761 acactccagc aatgaattcg aggccatgcg gttagcgccg tgtaagccgg tataactcac
     2821 ctcgccaatg gcatacaagc cctcgacgtc cgtacgccca tgatcatcca ccatcacacc
     2881 accgcaggta tagtgtgcag caggcacaat cggtaccggt tcttgtgtga gatcaatccc
     2941 cagcccgagc aatttttcat agatcatcgg gaaatgctgg cgaataaaat cggcaggctt
     3001 atggctgatg tcgagataca tacaatccgc gccgaggcgt ttcatttcat ggtcaatggc
     3061 gcgggcgaca atatcgcgcg gggccagttc gccgcgctca tcaaaatcgg gcataaaacg
     3121 cgtaccgtcc gggcgcttga gataagcgcc ttcgccgcgc agtgcttctg ttaacaggaa
     3181 attgcgtgcc tgtgggtgat acagcgcggt agggtggaac tgattaaatt cgagattggc
     3241 aacccggcag cctgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt
     3301 ggtggtgtac tgataaacct tcgacgcacc gccggttgcc agcaccaccg cttttgcgtg
     3361 gcaggtttca accgtttctt tatttcggtt ccatacccac gcgcctacaa ctcgtcgcgt
     3421 gcccggcagg ccaattttgt cagagacaat tagatcaacc gcgttgctgc gctccagcac
     3481 gcgaatattc ggatggttca gcgccttgct caccagcgtg gtttctactt ctctaccggt
     3541 ggcgtcggcg gcatgaagaa ttcgacggtg actatgtcca ccttcacggg tcagatggta
     3601 gctttctgca ccattcggtt gaatgtgggt atcaaacaac accccctggt cgattagcca
     3661 ttgcacacag gatcgtgcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc
     3721 acccccggca atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac
     3781 ggcggcaata ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag
     3841 aacgatgacc tgatgctgat cagccaggcg cagcgccagt gaaagtccgg ctgcgccgct
     3901 accgataatc aacacgtcac atgaatgatc agggagagta ttcattttct ttgtttaatt
     3961 tactaaacac ggtttggtca gcatagcatc atgttgtgcg attaaacacc tgctatttta
     4021 atatttgtta cagtggctaa acacgctgac gcagggcggc gagaaaaaga gaagttactg
     4081 gctggtggag gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa
     4141 atgggcattt ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata
     4201 atgatccgtc tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga
     4261 gacactctaa ccctttgctt gctcaaattg cagctaatgg agtggcgttt cgatagcgcg
     4321 tggaaatttg gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc
     4381 tggttgaacg ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc
     4441 agcataaagt ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg
     4501 tacaagaagc ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt
     4561 tttatacatg gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg
     4621 ggcgtcgtcc accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg
     4681 gcgcgttgaa agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga
     4741 tagttttccg aactattgag tccctcccgg aagatttacg catggcaata accttgcggg
     4801 agctggatgg cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg
     4861 tgcgttcacg tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca
     4921 ggcgttgacg atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc
     4981 cgctttaatg gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc
     5041 agaaatgcag aaaacctggg aaagctatca cttaatccgt gactcaatgc ggggtgatac
     5101 tcccgaggtg ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc
     5161 agtacgtcaa ccggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcagaa
     5221 aatgccattc tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc
     5281 agcatgcgta tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac
     5341 gtcccagcag cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc
     5401 ggtaagcctg ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca
     5461 ggagcagcgt cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca
     5521 ctctgaacag cttcagtttg agcaggcgca aactcagcaa gccgctgtac aggtgccagg
     5581 aattcaaact ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta
     5641 gtgacaggta gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta
     5701 ttacagcaga tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc
     5761 aataaacagg gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt
     5821 gcacaattgt tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc
     5881 agctattttg aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct
     5941 ctaccatcgc tcatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc
     6001 gtcgggcgca cgcgtattgc tgatcgtctt tgcgaagtca ttcgtgtggt tgcccgagat
     6061 ggtacacgct acagctacat cgtgtggatg gacaccgaat cgaaattacc gatgcgggtt
     6121 gatctccttg atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc ttttaatgtc
     6181 aatcaggata tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt
     6241 tctgttcctg taggtgaaaa agctaaattc agctggacgc caacctggtt gccacagggt
     6301 tttagcgaag tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa
     6361 tcacgtctct attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca
     6421 tcgagcaccg atcagatgtt gcgcaccggg cgcagaaccg tcagtacaag cgtacgtgat
     6481 aacgccgaaa tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag
     6541 aatattaagt tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctgggaaa
     6601 acgggcaggc gctggttagt tgtgatgtta aagcctcatg cagcagctgc gcttcacgtg
     6661 ccggttgcgg tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg
     6721 taccctgtga tgagccgtta gtgccggggc aaaaagtaga attaggaatc gccgaaggca
     6781 gcctgcttag ctccgcatta ctggtttata tgtcgccgtt ggtgggacta ttcctcatcg
     6841 cttcactatt tcagctactc tttgcttcag atattgcagc attatgcggt gcgattctcg
     6901 gtggcgttgg tggattcctg attgcacgtg gctgctctcg aaagtttgct gcccggtctg
     6961 aatggcaacc gatcatctta agcgtggcct tgccgcctgg ccttgtgcga tttgaaacat
     7021 cttcggaaga cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg
     7081 aggacgtttc cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta
     7141 gtgtagaatg cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa
     7201 gcctggttaa ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt
     7261 ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat
     7321 ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga
     7381 gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga
     7441 cggcgaaacc tatcagctta actttatcga caccccgggc cacgtagact tctcctatga
     7501 agtttctcgt tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg
     7561 cgtagaagcg caaaccctgg caaactgcta caccgccatg gaaatggatc tcgaagtggt
     7621 gccggtactg aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat
     7681 tgaagatatc gtcggcatcg acgccaccga cgcggtgcgc tgttcagcga aaaccggcgt
     7741 tggcgtacag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc
     7801 ggaaggcccg ttgcaggcac tcattatcga ctcctggttt gacaactacc tgggcgttgt
     7861 ttcacttatc cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag
     7921 taccgggcag acctataacg ccgaccgtct gggtatcttc acgccaaaac aggttgaccg
     7981 cactgaactg aaatgtggcg aagtaggttg gctggtatgt gcgattaaag atatccacgg
     8041 cgctccagta ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg
     8101 ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta
     8161 tgaagccttc cgtgacgcgc tgggcaaact cagcctgaac gatgcttcac tgttctatga
     8221 gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttcctcg gcctgctgca
     8281 catggagatc atccaggaac gtctggaacg tgaatacgat ctggatctga ttaccactgc
     8341 gccgaccgta gtgtatgaag ttgaaaccac gtcaagagaa gttatctacg tcgacagccc
     8401 atccaagctg ccagcggtaa ataacatcta cgaactgcgc gagccgattg cagaatgtca
     8461 catgctgctg ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg
     8521 cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat
     8581 ggcggaagtt gtgctcgatt tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc
     8641 tctggattac aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat
     8701 caacggtgaa cgtgttgatg cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg
     8761 cggtcgcgag ctggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc
     8821 cattcaggca gcgattggta cgcacatcat tgcgcgctcc accgtgaaac agctgcgtaa
     8881 aaacgtactg gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa
     8941 gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gtcgagctgc cgcaggaagc
     9001 gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc
     9061 gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt
     9121 ggataaattc tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc
     9181 cggtgactca ctggataaag caacactgaa aaaggttgcg ccgaagcctg gctggctgga
     9241 aactggtgct tctgtttttc cggtgctggc tatcgtattg attgtgcgtt cgtttattta
     9301 tgaaccgttc cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat
     9361 tctggtagag aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga
     9421 aaccggtcat ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct
     9481 tgattacatc aagcgcgcgg tgggtttacc gggcgataaa gtcacttatg atccggtctc
     9541 aaaagagctg acgattcaac cgggatgcag ttccggccag gcatgtgaaa acgcgctgcc
     9601 ggtcacctac tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg
     9661 tggggaagcg accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat
     9721 tcgtctttcc gagcgtaaag agacactggg tgatgtgacg caccgcattc tgacagtgcc
     9781 gattgcgcag gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg
     9841 gattgttccg ccgggacaat acttcatgat gggtgacaac cgcgacaaca gcgcggacag
     9901 ccgttactgg ggctttgtgc cggaagcgaa tctggtcggt cgggcaacgg ctatctggat
     9961 gagtttcgat aagcaagaag gcgaatggcc gacaggtgtg cgcttaagtc gcattggcgg
    10021 catccattaa tagcccatct tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat
    10081 ttatgagata aatctcccgt ggctaacgac atcccccgtc gttgtgtata gaatattccc
    10141 ccgaagttta aggttggcac ctccaggttg ccacggcaca cgaaacagcg ttggtcccca
    10201 tataccggta aactgaaact gcagcgaagc agttagcaga gccatgtata tcaggtctgt
    10261 ttcgtgtgct gaattgttga cgcatttatt tattggtatc gcatgaaccc catcgtaatt
    10321 aatcggcttc aacggaagct gggctacact tttaatcatc aggaactgtt gcagcaggca
    10381 ttaacccatc gtagtgccag cagcaaacat aacgagcgtt tagaattttt aggcgactct
    10441 attctgagct acgttatcgc caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc
    10501 gatatgagcc ggatgcgcgc cacgctggtc cgtggcaata cgctggcaga actggcgcgc
    10561 gaatttgagt taggcgagtg cttacgttta gggccaggtg aacttaaaag cggtggattt
    10621 cgtcgtgagt caattctcgc cgacaccgtc gaagcattaa ttggtggcgt attcctcgac
    10681 agtgatattc aaaccgtcga gaaattaatc cttaactggt atcaaacccg tctggacgaa
    10741 attagcccag gtgacaaaca aaaagatccg aaaacgcgct tgcaagaata tttgcagggt
    10801 cgccatctgc cgctgccgac ttatctggtc gtccaggtac gtggcgaagc acacgatcag
    10861 gaatttacta tccactgcca ggtcagcggc ctgagtgaac cggtggttgg cacaggttca
    10921 agccgtcgta aggctgagca ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa
    10981 tgagcatcga taaaagttac tgcggattta ttgctatcgt cggacgtccg aacgttggca
    11041 aatccacatt gttgaacaaa ctgctggggc agaagatctc cattacctcc cgcaaggcgc
    11101 aaacaactcg tcaccgcatt gtggggatcc atactgaagg cgcgtatcag gcgatctacg
    11161 ttgatacccc aggcctgcat atggaagaaa aacgcgccat taaccgcctg atgaacaaag
    11221 cggcgagcag ctctatcggc gacgttgagc tggtgatttt tgtcgtagaa ggcacccgct
    11281 ggacgccgga cgatgaaatg gtgctcaaca aactgcgcga tggcaaagcg ccggtaatcc
    11341 tcgcggtgaa caaagtggat aacgtgcagg aaaaagccga tctgctgcca cacttacagt
    11401 tcctggcaag ccagatgaac ttcctcgata tcgtgccgat ctctgccgaa accgggttga
    11461 atgttgatac tattgcggca atcgtgcgta agcatctgcc tgaagcgact catcacttcc
    11521 cggaagatta catcaccgat cgctcacagc gttttatggc gtctgaaatc atccgcgaaa
    11581 aactgatgcg tttcctcggc gctgaactgc cgtactccgt gaccgtggag atcgaacgtt
    11641 tcgtctctaa cgaacgcggc ggttatgaca tcaacggttt gattctcgtt gagcgtgaag
    11701 ggcagaagaa gatggttatt ggcaacaaag gggccaagat caaaaccatc gggattgaag
    11761 cgcgtaaaga catgcaggaa atgttcgaag cgcctgttca ccttgagctg tgggtaaaag
    11821 tgaaatccgg ttgggccgac gacgaacgcg cactgcgcag tctcggttac gttgacgatc
    11881 tttaagagta actccgatgg aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg
    11941 gagcgaaacg agcctgatgc tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt
    12001 tgccaaaggc gcacgctcta aacgctctac tctgaaaggt gcattacaac ctttcacccc
    12061 tctcttgctt cgcttcggtg ggcgtggtga agtcaaaaca ctgcgcagtg ccgaagctgt
    12121 ctcgctggcg ctgccattaa gcggtatcac cctttacagc ggtttgtaca tcaacgaact
    12181 tctttcccgc gtgctggaat acgagacgcg cttctctgaa ctctttttcg attacttgca
    12241 ctgcattcag tctcttgcag gtgccactgg tacgccagaa cccgcgctgc gccgctttga
    12301 actggcactg ctcgggcatc tgggctatgg cgtcaatttt acccattgtg cgggtagcgg
    12361 cgagccggta gatgacacca tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag
    12421 cgtcgttatc gacaataaaa cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga
    12481 atttcctgac gcagacacac tacgcgccgc gaaacgcttt acccgcatgg cgcttaagcc
    12541 gtatcttggc ggtaaaccat taaagagcag ggaactgttc cggcagttta tgcctaagcg
    12601 aacggtgaaa acacattatg aatgatgagg attgtcatgg ctgaattact gttaggcgtc
    12661 aacattgacc atattgcaac gctgcgtaac gcacgcggta ctgcttaccc ggatccagtg
    12721 caagcggcgt ttattgccga gcaggcggga gcagacggca ttaccgtgca tttacgtgaa
    12781 gatcgccgcc acattactga ccgcgatgtg cgcatcctgc gtcagacgct ggatacccgc
    12841 atgaatctgg agatggcggt gaccgaagag atgctggcga tcgccgttga gacgaagcca
    12901 catttttgct gtctggtacc ggaaaagcgt caggaagtaa caaccgaagg cggcctggac
    12961 gtcgcagggc agcgtgacaa aatgcgcgat gcctgtaaac gtctggcgga ggccgggatc
    13021 caggtttctc tgtttatcga cgccgatgaa gagcagatca aagctgcggc agaggttggc
    13081 gcaccgttta tcgagatcca caccggttgc tatgctgatg ccaaaactga cgccgaacag
    13141 gcgcaagagc tggcgcgtat cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa
    13201 gttaacgctg gtcacggtct gacctatcac aacgtgaaag ccattgccgc catccctgag
    13261 atgcatgaac tgaatatagg tcatgccatt attggtcgtg cagtgatgac cggactgaaa
    13321 gatgcggtgg cagaaatgaa gcgtttgatg ctggaagcgc gtggctaatg gcaatattag
    13381 gtttaggtac ggatattgtg gagatcgctc gcattgaatc ggtgatcgcc cgatccggtg
    13441 agcgtctggc acgccgcgta ttaagcgata acgaatgggc aatctggaaa acgcaccacc
    13501 agccggtgcg ttttctggcg aagcgttttg ctgtgaaaga agcggcggca aaagcgtttg
    13561 gcactgggat ccgcaatggt ctggcgttta atcaatttga agtatttaat gatgagctcg
    13621 gcaaaccacg gctacggcta tggggtgagg cattaaaact ggcggaaaag ctcggcgttg
    13681 taaatatgca tgtaacgctg gcagatgaac gccactatgc ttgtgccacg gtaattattg
    13741 aaagttaaaa aaataccgtt ctctttataa agtaaaaccc ccgaactggt aaggaacggg
    13801 ggttttactt tttaacttta attttgtgca gcacgcaccc tcaacaagag cgattatgga
    13861 tcagggcggt tatcttttac aagcaacttg caatctttag cataaaaact cgagccttta
    13921 cgaagaaaac aatattgatg gaaagattaa cgtgaccgcc aattcggaag tacattaaaa
    13981 ttggcttcgt tattggcgat tttgctgtgc tttataccat gccacagaat tcccccattg
    14041 aaacgagtgg tgtcgtcaaa gctctggtgt ggagtgcagc atgcaccctc aataactcgc
    14101 acgttcagtt ttggggagat gtaagggcta atctgaacgg ctgcattcct tgtttaagga
    14161 aaaacgaatg actgattgcc gataccttat taaacgggtc atcaaaatca tcattgctgt
    14221 tttacagctg atccttctgt tcttataaca taaggaaacg tacttaaggc gcgtcaggtt
    14281 aaccagccag gcgcgc
//
LOCUS       DAYPHN010000128        14154 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000128 DAYPHN010000000
VERSION     DAYPHN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.128"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(30..938)
                     /gene="melR"
                     /locus_tag="ACG0BW_003524"
     CDS             complement(30..938)
                     /gene="melR"
                     /locus_tag="ACG0BW_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFK7260448.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1221..2576
                     /gene="melA"
                     /locus_tag="ACG0BW_003525"
     CDS             1221..2576
                     /gene="melA"
                     /locus_tag="ACG0BW_003525"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFK7260449.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDDLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2679..4100
                     /gene="melB"
                     /locus_tag="ACG0BW_003526"
     CDS             2679..4100
                     /gene="melB"
                     /locus_tag="ACG0BW_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFK7260450.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4239..4868)
                     /locus_tag="ACG0BW_003527"
     CDS             complement(4239..4868)
                     /locus_tag="ACG0BW_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFK7260451.1"
                     /translation="MTRTLKPLILNTSALTLTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGHKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4991..6637)
                     /gene="fumB"
                     /locus_tag="ACG0BW_003528"
     CDS             complement(4991..6637)
                     /gene="fumB"
                     /locus_tag="ACG0BW_003528"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFK7260452.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLERNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6715..8055)
                     /gene="dcuB"
                     /locus_tag="ACG0BW_003529"
     CDS             complement(6715..8055)
                     /gene="dcuB"
                     /locus_tag="ACG0BW_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFK7260453.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8626..9345)
                     /gene="dcuR"
                     /locus_tag="ACG0BW_003530"
     CDS             complement(8626..9345)
                     /gene="dcuR"
                     /locus_tag="ACG0BW_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFK7260454.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9342..10973)
                     /locus_tag="ACG0BW_003531"
     CDS             complement(9342..10973)
                     /locus_tag="ACG0BW_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFK7260455.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11154..11384
                     /gene="yjdI"
                     /locus_tag="ACG0BW_003532"
     CDS             11154..11384
                     /gene="yjdI"
                     /locus_tag="ACG0BW_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFK7260456.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            11396..11668
                     /locus_tag="ACG0BW_003533"
     CDS             11396..11668
                     /locus_tag="ACG0BW_003533"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7260457.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11897..12193
                     /gene="ghoS"
                     /locus_tag="ACG0BW_003534"
     CDS             11897..12193
                     /gene="ghoS"
                     /locus_tag="ACG0BW_003534"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFK7260458.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12221..12394
                     /gene="ghoT"
                     /locus_tag="ACG0BW_003535"
     CDS             12221..12394
                     /gene="ghoT"
                     /locus_tag="ACG0BW_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFK7260459.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12512..14029)
                     /gene="lysS"
                     /locus_tag="ACG0BW_003536"
     CDS             complement(12512..14029)
                     /gene="lysS"
                     /locus_tag="ACG0BW_003536"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFK7260460.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         3485 a         3370 c         3564 g         3735 t
ORIGIN      
        1 tatttttttg gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg
       61 ctaagtttgc ggtattgttg cggtgacatg ccgacatatt tgccgaaagt gctgtaaaaa
      121 cggctactcg aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg
      181 ctcagtaacg cgcgaacgtg gttgatgcgc atcgcggtta tgtactgttt catcgtcaat
      241 tgcatgaccc gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc
      301 acatcgttga tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta
      361 acataaaatt gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc
      421 agaatcggtt cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg
      481 gcgagctggc gaatttgctc gttcggactg ttcaattcct gctgccagcg gcgcacttca
      541 aacgggctaa gttgctgtgt cgccagtgat ttgatcacca taccgtgagt gacgtggtta
      601 atcaggtctt tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc
      661 atgctctgac aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg
      721 atatgaccct gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg
      781 aaaggcacat tcacttcgac ctggccatgc cagtggctgg tgggcatgat atgcggtgcg
      841 cgaaactcaa tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt
      901 ttttcgtcgc tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat
      961 atcagaatta tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct
     1021 cttcccacag cggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag
     1081 gtaatttatt cccataaact cagatttatt gatgcttcac gtaggatctg agtttatggg
     1141 aatgctcaac ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc
     1201 aagcaagcca ggagatctgc atgatgtctg cacccaaaat tacatttatc ggcgctggtt
     1261 cgacgatttt cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg
     1321 cgcatattgc cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc
     1381 gcaagctgat ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag
     1441 aagccttaca ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt
     1501 gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata
     1561 cgttggggcc gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt
     1621 gcgaggacat gacggaagtc tgccccgatg ccaccatgct caactacgtt aacccaatgg
     1681 cgatgaatac ctgggcgatg tatgcccgct atccgcatat caaacaggta gggctgtgcc
     1741 attcggtgca gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccggctacgc
     1801 tgcgttaccg ttgcgcaggt atcaaccata tggcgtttta cctggaactg gagcgcaaaa
     1861 ccgccgacgg cagttacgtg aatctctacc cggaactgct ggcggcttat gaagcagggc
     1921 aggcaccgaa gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctatgaaa
     1981 tgttcaaaaa gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac
     2041 cgtggtttat taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg
     2101 agtacccgaa acgctgcgtc gagcagttgg cgaactggca taaagagctg gaggagtata
     2161 aaaacgcctc ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg
     2221 ctatctggac tggcgagccg agtgtgattt acggcaacgt ccgtaacgat gatttgattg
     2281 ataacctgcc acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc
     2341 agccgaccaa agtcggtacg ctaccttcgc atctggccgc tctgatgcaa accaacatca
     2401 acgtacagac gctgctgacc gaagccattc ttacggaaaa ccgcgaccgt gtttaccacg
     2461 ccgcgatgat ggacccgcat actgccgccg tgctgggcat tgacgaaata tatgctcttg
     2521 ttgacgacct gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg
     2581 cgactaaacg ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata
     2641 acagaaggcg ggcgttggta acaacgaccc gataccctat gagcatttca atgactacaa
     2701 aactcagtta tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt
     2761 acctcatgta ttactacacc gatgtcgtcg ggctgtctgt gggtttggtc ggtactttgt
     2821 ttctggtggc gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg
     2881 ctacgcgatc gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact
     2941 ctgtaatctt atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct
     3001 ttgtttgcgt gacctacatc ctctggggca tgacttacac cattatggat attcccttct
     3061 ggtcgctggt tccaaccata acgctcgata aacgtgagcg cgaacaactg gttccttatc
     3121 cgcgtttttt tgccagtctg gcgggctttg ttacggcagg tgtgacgcta ccatttgtta
     3181 attatgtcgg cggtggcgat cggggatttg gctttcagat gttcactctg gtactgatcg
     3241 ccttttttat tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag
     3301 acaatcaacc gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgcttattt
     3361 ataaaaacga tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca
     3421 atattattac cggctttgct atctattatt tctcatatgt tatcggtgat gcggatttgt
     3481 tcccctatta tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac
     3541 gcttagttaa atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt
     3601 taagctgtgg tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg
     3661 tgattgcggg tatcttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca
     3721 tggtggcaga taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagcatcg
     3781 cttactccgt gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg
     3841 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
     3901 taggtatgca gtttattatg attgctctac cgactctgtt tttcatggta acgctgattc
     3961 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
     4021 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
     4081 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
     4141 ttatcaggcc atgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
     4201 aattcacgcc gcatccggcg tcaacaaagc gcacgttgtt atccggttat caagccaaag
     4261 cgccgtagct ggcggcaatg aaagcgagcg agaaatatca ccgtcgttaa tgcgccaagc
     4321 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
     4381 tccgctccct ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt
     4441 atcgccagcg ccacgcagca gaccagaaac gccaccatct tatgtccgcg aacgtacatc
     4501 ccgcgcacaa ggatctctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc
     4561 agtttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
     4621 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaaa gaaaatgagc
     4681 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
     4741 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
     4801 taaatcagga ttaacgttag cgtcagtgcg ctggtgttaa gaattaacgg cttgagtgtg
     4861 cgggtcatgg tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa
     4921 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     4981 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     5041 cgttgccttt gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga
     5101 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     5161 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     5221 cgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     5281 attgcagcag atccacgtag gagtccatac gacctgcggt ggttgggcca agtgaacctg
     5341 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     5401 actgcggcag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     5461 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccggatat tgcgaaagct
     5521 gggcgaggat ctctttcatc gggcggttaa ggtcgacctt caccgcttcg ccttcaccgg
     5581 cctggcgcag ttcttgtgga atgtactggc ctggattgcg ttccagtttt tcgatccaga
     5641 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     5701 ctaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     5761 atttaccacc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     5821 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcat
     5881 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     5941 tcacaaacgc gatatggtac ggcgggcagg ctgccgtacc gagggtacgc attttctcaa
     6001 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatagg
     6061 ttttgttggc agagccgccg cctttagcaa cacagaggaa tttgtactca tcgccatcta
     6121 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     6181 ccagcgccgc gttttgcgag tagcgcaggt tatcctcgat ataggtgtta tagacgcctt
     6241 tcgacagcgc ttcttcatca ccgccaccgg tccacacgcg ctggcctttt ttaccgacga
     6301 tgatcgcggt gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt
     6361 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     6421 tagccgcaac ctgtttctgg tgtgccggac ggagcatgaa agaagcgtcg tgaaaggctt
     6481 gctgcgccag cagggtcagg gcttctggtt ccactttcag aatggtttcg ccgtcgaagt
     6541 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     6601 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     6661 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     6721 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     6781 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     6841 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     6901 agccggtgct gaagccacga tgtatgctgg atcaacgccg atcgccagcg cgaccggaac
     6961 aatcgccgcc agcgccgcag cctgagagtt tacaaactta gaaaccagca gcagaacaat
     7021 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     7081 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     7141 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     7201 aataatcagc gccccggtaa gcagcataaa catctgaata accagtacca tcgacagcgg
     7261 tttgccaccg aaggatggac gcaggtccga atcagcacca agaagtgcga ctacagcgat
     7321 tgccccgagg aagatccaca ttgcaagcca gttgcttttc ggcagttttt tatcgagcag
     7381 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     7441 ctcttcgtct ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat
     7501 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     7561 atcaaaggtg acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact
     7621 ggcgataatc cccatctgtg caccgataga acttgccgcc atcgggcgtt ccggacggat
     7681 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     7741 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     7801 gttgcggcgc agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     7861 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     7921 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
     7981 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     8041 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     8101 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     8161 tcgttaccgg ctttagcaaa tacctcacag tgaatattgg ctgattaaag cagcggcaaa
     8221 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     8281 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     8341 tattttttgt tctcagggaa gatatcatta ggtttataga ttaatctgat ctacccattt
     8401 gtgggtaaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     8461 tgaaattaaa aacaccgttc atctgaagag atattaattt ttaacgatga tggagggata
     8521 attatatttg atctggcaca agttttactg atgaagaatg taacttgtgc caggggtatt
     8581 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     8641 tttcagcagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccctgtgac
     8701 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtattttcg
     8761 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     8821 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     8881 caagcgacga ggatcctgct cgttggagct gctgccgtgg attagctgat caagctcggc
     8941 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     9001 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacaacac cgtaatgcag
     9061 ggaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     9121 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     9181 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     9241 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     9301 ctccgcgacc attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc
     9361 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     9421 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     9481 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     9541 ggtccatcat cgttaacttc acagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     9601 atttcgcctc cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     9661 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagttg gctttcactg
     9721 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     9781 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg
     9841 gctgttttga gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     9901 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcgtc agcatagttg
     9961 accagaccgt cgagtcgctg catcagttta cgtacttcag ttttgtctct gaaggttgaa
    10021 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
    10081 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
    10141 tctactacct gtgaccacga gtggcttagc gtcgacagct tctcatcgtc ctgcgactta
    10201 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacttcacc gcgatcgtcc
    10261 acggcaacga cgccttcttt gatggactgc aacatggcct ggcgctgctc aaacagcgtg
    10321 gagatttcgt agggttccag gccaaaaaga atttttttca gtactttaac cagaatgcag
    10381 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
    10441 tcattgatct gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgc
    10501 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
    10561 ttgatagcga cattttcttc gccattcagc gctttaagga tgtcatcacc tttaaatggc
    10621 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca
    10681 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat gccgctctcc
    10741 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccacc
    10801 gccagtgcct tgttggctag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc
    10861 agatgcacca ccaatagcac cgagaacagt accgcgctga ccattaagat cacagtggta
    10921 ctcaatttca tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc
    10981 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
    11041 gaatgattgg ctatataggg gaagagattc tggcaacgga aactgccagt gctgtatgaa
    11101 gattccaggg ctatgcttat agtgaaaacc atactgatga gagagggaag gtcatggatc
    11161 gggcgctact ggacgggggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
    11221 atactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
    11281 tcaaacgtaa gccgtggatc atgccggatg aagtcgacgt cgccactgtg gtgaaagtga
    11341 ttgatacgtg cccgagtggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
    11401 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
    11461 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
    11521 aagcctgaaa gggcaaggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
    11581 tcgggaaaaa cgaaaaatta tcccattatg tccatttgcg aaacacgaat ttgataaaac
    11641 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
    11701 ctctccgcgg ggtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatggtc
    11761 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa aaagaatatc
    11821 tcctatatga gaatcatcga tcggggttaa taagttttgc gtccccagag cgtttaaaat
    11881 tgataggagt catattatgg aaggtaaaaa caagttcaat acttatgttg tttcttttga
    11941 ttatccatca tcttattcct cagtgttctt aaggttaaga tcattgatgt atgatatgaa
    12001 tttctcctct atcgtggctg atgaatatgg gataccacga caattgaatg aaaattcctt
    12061 cgcaataacg acatcgttag ccgcaagtga aatcgaagat ttaatcaggc ttaaatgctt
    12121 agacttaccg gatattgatt ttgacctcaa cattatgacc gttgatgact atttccgtca
    12181 gttttacaag tagccaggaa ggataagaag aacataaaaa atggcactat tctctaaaat
    12241 attaattttt tatgtgattg gtgtgaacat atcctttgtc attatctggt ttatctcaca
    12301 tgagaaaaca catattcgtt tacttagtgc attcctggta ggaataacct ggccaatgag
    12361 tctgcctgtg gcattacttt tttctctctt ttaggagctg tagttgagtg gacccgctgg
    12421 ttatcttgaa acgaagcgtc tctcctcaaa actggatttg cggaacgacg aacgaagcag
    12481 tcgcctgact gcttcgttca tttagtgaaa tttatttctg tgggcgcatc gctgggaaga
    12541 gaataacgtc gcgaatagta tggctgttag taaacagcat aatcattcgg tcgataccaa
    12601 tacccagacc agcggttggc ggcagaccat attccagcgc agtcacgtaa tcttcgtcat
    12661 agaacatggc ttcgtcgtca cctgcagctt tagcgttaac ctgttcctgg aaacgttcag
    12721 cctggtcttc tgcgtcgttt aattcgctaa aaccattacc gatttcacga ccaccgatga
    12781 agaattcaaa acggtcggtg atttccgggt taacatcatt acggcgtgcc agcggtgaca
    12841 cttctgccgg atattccgta ataaaggttg gctgaatcag atgtgcttct gccacttcat
    12901 caaagatctc cgtcacaata cgtcccagcc cccagctttt ctctaccgta ataccgatag
    12961 attcagctaa tgctttagca gcatcaaaat tatccaggtc ggccatatcg gtttctggac
    13021 gatatttttt gattgcttcg cgcatggtaa gtttttcaaa cggtttgccg aaatcaaaca
    13081 catgctcgcc ataagtgact ttagtggtac caagaacctc ttgtgccagg gtgcggaaca
    13141 gggactccgt cagttcaatc aaatcgtggt aatccgcata cgccatgtag agttccatca
    13201 ttgtgaactc aggattatgg cgaacagaaa taccttcatt acggaagtta cggttgattt
    13261 cgaatacccg ttcaaaaccg cctacaacca gacgtttcag atacagctcc ggcgcgatac
    13321 gcagatacat atctaaatcc agagcattat gatgggtaat aaacgggcga gcagatgccc
    13381 cacctggaat tacctgcatc atcggggttt ctacttccat aaagccgcgc gcgaccatga
    13441 actgacggat agcggccaga atttttgaac ggacaacaaa cgtttgacgg gatttatcgt
    13501 tagcgatgag gtccagataa cgttgacgat aacggacttc ctgatcctgc agaccatgga
    13561 atttatctgg taaaggacgt agtgctttag tcagcaggcg cagctcagta cagtgaatgg
    13621 aaagctcacc cgtttgcgtc ttaaacagcg taccgcgggc accgataatg tcacccagat
    13681 cccatttttt aaactgatcg ttataaacac cttctggcag gctatctctt gcaacgtaca
    13741 gttgaatacg gccaccgaca tcctgcaacg ttacaaagga ggctttcccc atgatacgac
    13801 gggtcatcat tcggccagca accgagactt caatgtttaa ggattccagt tcctggttat
    13861 ccttcgcatc aaactcttcg tgcaactggt cagaggtatg gtcgcggcga aaatcattgg
    13921 gaaacgccac accttgctga cgcagtgccg ccagtttttc gcggcgattt ctcagttcat
    13981 cgttaaaatc aatagcctca ttggctcccc gtgtttctcg ttcagacatt ttggttcctc
    14041 taaatccagc tttcaattta gcgtagataa agagacagat cggtacactg tattatctgc
    14101 ctcgactatt aataactcag tttatcttat cgaaactaac gcttacctca agtt
//
LOCUS       DAYPHN010000129        13988 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000129 DAYPHN010000000
VERSION     DAYPHN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.129"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            55..621
                     /locus_tag="ACG0BW_003537"
     CDS             55..621
                     /locus_tag="ACG0BW_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7260461.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            672..3131
                     /locus_tag="ACG0BW_003538"
     CDS             672..3131
                     /locus_tag="ACG0BW_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFK7260462.1"
                     /translation="MDTVNIYRLSFISCLVMAIPSALAVEFNLNVLDKSMRDRIDISL
                     LKGKGGIAPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDN
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDNDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSNHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVADNVIKETWIEGAIGYKSLASRSGK
                     DVNLIIRNASGQFPPLGADIRQEDSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSIHLPEHMEDTANRLILPCH"
     gene            3147..3878
                     /locus_tag="ACG0BW_003539"
     CDS             3147..3878
                     /locus_tag="ACG0BW_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFK7260463.1"
                     /translation="MTFMKGLPLLLLIASLCSYAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPSALRKKAGEKVELQLQVSQQG
                     NQLNLKNPTGYYLTIAYLGRNEQGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCNGHCRLQAVEAKK"
     gene            3875..4936
                     /locus_tag="ACG0BW_003540"
     CDS             3875..4936
                     /locus_tag="ACG0BW_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7260464.1"
                     /translation="MSTGKGLLLVICLLFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHNMTKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLISNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5088..6134
                     /locus_tag="ACG0BW_003541"
     CDS             5088..6134
                     /locus_tag="ACG0BW_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFK7260465.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLTILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6131..6922)
                     /gene="nei"
                     /locus_tag="ACG0BW_003542"
     CDS             complement(6131..6922)
                     /gene="nei"
                     /locus_tag="ACG0BW_003542"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFK7260466.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSQLIGQH
                     VTHVETRGKALLTHFSNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLSAAQLDALAHALLDIPRLSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6958..7692)
                     /gene="pxpA"
                     /locus_tag="ACG0BW_003543"
     CDS             complement(6958..7692)
                     /gene="pxpA"
                     /locus_tag="ACG0BW_003543"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFK7260467.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRRAMQLPPETVYAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALEQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7682..8614)
                     /gene="pxpC"
                     /locus_tag="ACG0BW_003544"
     CDS             complement(7682..8614)
                     /gene="pxpC"
                     /locus_tag="ACG0BW_003544"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFK7260468.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8608..9264)
                     /gene="pxpB"
                     /locus_tag="ACG0BW_003545"
     CDS             complement(8608..9264)
                     /gene="pxpB"
                     /locus_tag="ACG0BW_003545"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFK7260469.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9287..10030)
                     /gene="ybgI"
                     /locus_tag="ACG0BW_003546"
     CDS             complement(9287..10030)
                     /gene="ybgI"
                     /locus_tag="ACG0BW_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFK7260470.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10301..11782
                     /gene="dtpD"
                     /locus_tag="ACG0BW_003547"
     CDS             10301..11782
                     /gene="dtpD"
                     /locus_tag="ACG0BW_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFK7260471.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11824..13242)
                     /gene="phrB"
                     /locus_tag="ACG0BW_003548"
     CDS             complement(11824..13242)
                     /gene="phrB"
                     /locus_tag="ACG0BW_003548"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFK7260472.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAKLINAQLNGLRIALAEKGIPLLFREVDDFAASVEIIKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGMIEPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQS
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALEGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIVWTDRVQWQRNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13239..13748)
                     /locus_tag="ACG0BW_003549"
     CDS             complement(13239..13748)
                     /locus_tag="ACG0BW_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFK7260473.1"
                     /translation="MNQQRFDESTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
BASE COUNT         3376 a         3723 c         3427 g         3462 t
ORIGIN      
        1 gttaatatat tcttgcctcg caacactcta tctcattatt taaaggactc agctatgttt
       61 aaaggacaaa aaacattggc cgcactggcc gtatctctgc tgttcactgc acctgtttat
      121 gcggctgatg aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt
      181 gaaatacatc aggatgatat tgataaagaa gttgaactcg gtcaggtgac caccagccac
      241 attaatcagt cgcatcacag cgatgccgtt gctgtcgacc tgcgcttagt caactgcgat
      301 ctggaaaact ccaacaacgg ttccggcggc aagatttcta aagttgcagt cacctttgac
      361 agctcagcga aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcg
      421 accggtgtcg gcgtgcgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct
      481 actccagata tcgacctggc accaacctcc agcgaacaaa cgttaaattt ctttgcgtgg
      541 atggaacaaa ttgatcaggc tacgcccgta acaccaggcg cagttacggc gaatgcgacc
      601 tacgtactgg attataaata agtatcactt tggcggcgtc atcgccgcct ttcttcaaat
      661 atttatcagg tatggacacc gtgaatattt atcgactctc ttttatatcc tgcctggtca
      721 tggcgatacc ttccgcattg gcggtcgaat tcaacctgaa tgtcctcgat aaatcgatgc
      781 gagatcgcat tgatatttca ttattaaagg gaaaaggggg cattgctccc ggtgaatatt
      841 ttgttagcgt tgcagtaaat aacaaccaaa tcagtaacgg acaaaaaatt gactggaaaa
      901 agaacggtga ccaaaccatt ccgtgtatta atgatttact ggtagataaa tttggtttaa
      961 aacctgaagt tcgtcagtcg ttaccacggt taaaccagtg cgtcgatttt agctcccggc
     1021 ctgaaattct cttcattttt gatcaggcta gccagcaact gaatatcact attccacaag
     1081 cctggctggc gtggcactca gataactgga ctcccccctc aacatggaaa gaaggtgtcg
     1141 ccggcgtcct gatggactac aacctgtttg ccagcagcta ccgcccacag gatggtagca
     1201 acagcactaa cctgaacgcc tatggaacag ccggaattaa cgcgggagca tggcgtttac
     1261 gcagtgatta ccaattgaat cagaccgata gcgatgataa ccatgaacag tcaggcgaaa
     1321 tatcacgcac ctatcttttt cgtccattac cgcaattagg ttcaaaatta acccttggtg
     1381 aaacggattt cagttccaat attttcgacg gcttttctta taccggcgcg gcactggcaa
     1441 gtgacgatcg gatgttaccg tgggagctac gcggctacgc cccacaaatc agcggcattg
     1501 cacaaaccaa cgccaccgtc accgtgagtc actcagggcg agttatttac cagaaaaaag
     1561 tcccaccagg tccgtttatc attgatgacc ttaatcagtc cgtccagggt acgttggatg
     1621 tcaaagtgac ggaagaagat ggtcgggtga acaatttcca ggtttcggca gcatcgacgc
     1681 ctttccttac gcgccagggg caggttcgct ataaacttgc tgccggtcag ccgcgaacat
     1741 ctatgtctca tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc
     1801 tgtcaaatac ctcactgtac ggcggcctgc tgatttcggg tgacaactac cattctgccg
     1861 caatgggtat cgggcaaaat atgctctggc ttggtgcatt gtcgtttgat gtcacctggg
     1921 ccagtagtca gtttgatact cagcaagacg agcggggctt aagttaccgc tttaattaca
     1981 gcaaacaagt ggatgccact aacagcacga tttcgctcgc cgcttatcgc ttctcagatc
     2041 gtcattttca cagctacgcc aactatctgg atcacaaata caacgacaac gatgcgcagg
     2101 acgaaaaaca gacgatcagc ttatccgtgg ggcaaccgat taccccactg aacctcaatc
     2161 tgtacgccaa cctgctgcat caaacctggt ggaatgcaga cgcctccacg accgccaaca
     2221 tcacggcggg ttttaatgtt gatattggta actggagaga tatctcgatc tcgacgtcat
     2281 tcaatacaac ccattacgaa gataaagatc gcgacaatca gatttacctg tcgatttcgc
     2341 tccccttcgg taacgggggg cgggtcggtt atgacatgca aaacagtaat cacagcacca
     2401 cgcaccgcat gtcgtggaat gatacgttag atgaacgtaa tagctggggc atgtctaccg
     2461 gactgcaatc cgaccgtccg gataatggag cccaggtgag cggtaactat cagcacctga
     2521 gttcagcggg tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca
     2581 gcagcagctg gagcggttct ttcacggcaa ctcaatatgg tgcggcgttc catcgtcgca
     2641 gctccaccaa tgaaccacgt ctgatggtca gtaccgatgg cgtggcagat attccggttc
     2701 agggcaatct cgactacacc aaccattttg gcattgcggt ggtgcctttg atttccagtt
     2761 accagccttc caccgtggcg gtgaacatga atgacttacc cgacggcgta acagtggcag
     2821 ataacgttat caaagaaacg tggattgaag gcgcgatagg ttacaaatcg ctggcttccc
     2881 gttccggtaa agacgttaac ctcatcattc gcaacgccag cggtcagttc cctcccctcg
     2941 gtgcggatat ccgccaggag gatagcggca ttagcgtggg aatggttggc gaggaaggac
     3001 acgcctggtt aagtggagtc gctgaaaatc aacaatttac cgtgatctgg ggtgatagcc
     3061 agcgttgctc gatccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac
     3121 cttgtcatta attaaggaaa tgaaaaatga catttatgaa aggacttccc cttttgttgt
     3181 tgattgccag tctgtgcagc tacgctgcgc tacaacccga tcgcactcgc attgtgttta
     3241 acgctaatga taaagccacc agcctacgag tggataatcg tagcgataaa ctcccctacc
     3301 tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag cgacgatctt ctggtcgccc
     3361 tgccaccgat acaacgtctg gaaccgaaag caacaacgca ggtgcggata gtcaagcaag
     3421 cctcgaccgc gaaattaccg ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa
     3481 ttcctccctc cccagaaaaa aacagcggcc atgccgttct gcaggtcgcc atccagagtc
     3541 gtatcaaggt gttctggaga ccgtccgcgt tacgcaagaa agcaggagaa aaagtcgagc
     3601 tacagttaca agtcagccag cagggcaatc aacttaactt aaaaaacccg accggctatt
     3661 acctgacaat cgcctacctg ggacgaaatg agcaaggtgt tctcccgggc ttcaaaaccg
     3721 tgatggttgc gccttttagt acagtaatta caaacaccgg aagttatagc ggcagtcagt
     3781 tttatctcgg ttatatggat gattacggcg cgttacgcat gacgacgctc aattgcaacg
     3841 gacattgccg tttacaagca gtggaggcga agaaatgagt actggcaaag gattgttgct
     3901 cgtcatctgt ctgttatttc tgccattgaa gtctgcgcta gcgctgaact gctattttgg
     3961 tacttccggc ggaacggtag aaaaatcgga agcgattcaa ccctttgctg tgccaggtaa
     4021 tgccaaaccc ggcgataaga tctgggaatc tgacgatatt aaaattcccg tctattgtga
     4081 caacaatacc aacggcaact ttgaaagcga acatgtctac gcatgggtaa acccctaccc
     4141 gggggtacag gatcgttatt atcaactcgg cgtgacatac aatggtgtcg attacgatgc
     4201 cagtctgggg aaaagccgca tcgacaccaa ccagtgtatc gacagtaaga acattgacat
     4261 ttatacccct gagcagatca tcgcgatggg atggcagaac aaaatttgtt cgggcgatcc
     4321 cgctaatatt cacatgtcgc gtacctttct cgctcgcatg cggttatacg tcaaaatacg
     4381 agaaatgccg ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg tgcaatttga
     4441 cggtgccggt agcgttaacg aagatcctac cgcccaaaac ctgaaatatc atattactgg
     4501 tctggaaaac attcgcgtgc tggattgcag cgtcaatttt tctatttcac cggaaacaca
     4561 ggtgattgat tttggtaaat ttaatttgct ggatattcgg cgacataaca tgacgaaaac
     4621 gttcagtatt aaaacgacca agagccaaaa cgatcaatgc accgacggat ttaaagtcag
     4681 ttcctcgttt tatactgaag aaacgttggt tgaagaagat aaagcattgt tgattagtaa
     4741 tggtctgaag ctacgtttac tggatgaaaa cgcctcgcct tatacattca ataaatatgc
     4801 tgaatacgcc gatttcacca gtgacatgtt gatctacgaa aaaacctata cagctgaact
     4861 ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc gatacagtgg tgctttttaa
     4921 gattaactat aactgatttg ccatgatacc ctttgcagcc ggaaagggca tctctttcag
     4981 aaaattgctg attttcccct tttccttgat tgctgttatt ttttcctggc ccggctttct
     5041 ttatgctgta tcgatacacc aaagaaaaaa taatgaggga acgaaggatg ccagttttgc
     5101 agtggggaat gttatgtgtg ctctcacttc tcctttctat tggtttcctc gcggttcatc
     5161 tcccggcggc gctattactt gggccgatga tcgccgggat catctttagt atgcgcggaa
     5221 ttactctgca acttccccgc tctgcttttc tcgccgccca ggccattctt ggctgcatga
     5281 ttgcgcaaaa cctcactggt tcgattctca ccacgctggc cgtcaactgg ccgatcgtgc
     5341 ttacaatttt actggtgacg ctgctttcca gcgccatcgt gggttggtta ttggtgcgct
     5401 atagctcact gcctggaaat accggtgcct ggggttcttc tcctggcggc gcggcggcaa
     5461 tggtcgctat ggcgcaagat tacggcgcag atattcgcct ggtggcgttt atgcaatatc
     5521 tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg ctgggcgata
     5581 acgctgaagc ggttaaccag cagatcgtct ggttcccgcc agtgagcatt aatctcctgc
     5641 ttacgatttt gctcgcggtc gttgccggta cggcgggatg cttgctacgt cttccttccg
     5701 gcacgatgct catcccgatg ctggcgggcg cagtgctcca gtctggtcag ctcatcacga
     5761 ttgaactccc ggaatggctg ctggcgatgg cgtatatggc aattggctgg cggattggtc
     5821 ttggtttcga taagcaaatc ttactgcggg cattgcgccc gctgccacaa atcttgctgt
     5881 cgatttttgc tctgctggct atttgtgcag gtatggcgtg gggactgacc cggtttatgc
     5941 atattgattt tatgactgcc tacctcgcca ccagtcccgg cgggcttgat accgtggcgg
     6001 tcatcgccgc agggagcaat gccgatatgg cgcttattat ggcgatgcaa accctgcgcc
     6061 tgttcagtat tttgctgacg gggccagcca ttgcgcggtt tatttcaacc tatgcgccga
     6121 agcggtcggc ctagtgctgg cagcctggac accagtaaaa cgggcgcgag gacaacgtgg
     6181 ttttctcaat gatgctgcca cagcgttcac acggttcgcc atcacgatga aaaaccttaa
     6241 agcgaaacaa cgccccatga tgtttattct catccacctg cccccgcgta gcgtaagaaa
     6301 gtcgaggaat atccagtaac gcgtgtgcga gtgcatccag ttgcgccgca ctgagatctt
     6361 tcgctttatg attgccggtt aaccccacct gccagaggat ctccacccgc aaataattgc
     6421 caagtcctgc cagaaacgcc tgatcgagca gtaatccagc aaactgacgg ttacgaaagc
     6481 gcggcgacaa taatcgttcc tttaccacct ccggcgtcag attcggatcc agcacatcgg
     6541 ggccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc cggggtcaac atctcaatat
     6601 ccgaggcgct ataaagcaga atggttttgt cagccgtttg cagttttacc cgcaatactc
     6661 gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca gacgccgtaa agctgattat
     6721 ggctgtagag cgttaaaccg ttggaaaaat gagttaacaa cgccttacct cgcgtttcca
     6781 catgggtaac gtgttgaccg ataagttgtg attgataaga ttttaactgc gggaaggcaa
     6841 accagacatc agttagtggt ttgcctttga tcgccgcctc caggttatcc gctgcacggc
     6901 ggatctccgg gccttcaggc atggtgatat ccttttttca ttctttatta agcggggtta
     6961 tgctgcgaca acaatcccct tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag
     7021 tgcgtgctcg ccatcgccat gcaggcagac cgtttgcgcc gtgaccgttg cccattcgcc
     7081 ggtgatgctt ttgactctgc cgtgttgcac catctccaac gtttgctcca gcgcctgttc
     7141 ttcgttttca atcaacgcgc ctggctggct tcgcggcacc agcgaaccgt ctgcctgata
     7201 accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat
     7261 cagctcgctt cctgccagcc cgacgagaat caatgccgga tcgcaagcgt ataccgctct
     7321 ggcgatggcg tctgccagtt gtgcctcttt cgccgcctgg ttgtataaca tgccgtgcgg
     7381 tttgacatga cacattacgc cgccttgcgc acgggtaatc gctgccagcg cgccaatttg
     7441 atacagcgtc tgggcgtaaa cggtttctgg cggcagctgc atggctctgc gaccaaaatt
     7501 ttccctgtcg ggaaaactcg gatgagcgcc aatcgcaaca ccatttttta ttgcttcacg
     7561 cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat ccacaggcaa tattggcaga
     7621 ggaaaccagc gttaatagct ctgcatcgct ggcatagcct tcgcccagat cggcgttcag
     7681 gtcaattttc attgtgcagc cgccacgcta attgttcgaa ataacgttgc tgatcttgcc
     7741 gtgctttcag tgcttcttcc agtgaacact ggacaaaatg aatcggctga ccgagcggaa
     7801 tttgcgccag atggtacata tcagcctcga tgatacaggc aatacgcgga taaccaccgg
     7861 tggtttgcgc atcgttcatc aacacaatcg gctggccgtt atgcggcact tgcaccacac
     7921 ccggcaacaa accgtgagat aacagttcgc gatcggtggt gcgttttaaa atttgcccct
     7981 gtaagcgata gcccatgcgg ttactttgcg gactaagctg ccacggcgaa cgccagaatg
     8041 catcctgcga ggcgcgatcg aactcatgat attccggccc cggcaaggcg cgaatgcggt
     8101 tgccccacag cagctgttta acgccctgcg cttccataaa atcacgcttc gatttgccaa
     8161 tcgggagtcg gtcaccatcc ttcagtaaac ggccttccaa cccaccaatc cccactttga
     8221 gatcggtgct gcatgacccc attaccggcg gaacatcaat accacccgcg accgccagat
     8281 aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca
     8341 atcgccagcc ggtccagacg gcattatcat ccagccgcgc ttcgcaacct gcccccgtca
     8401 gggcaaacca cccatcagtt tcgaactcta ccgttaactg accgagcgtg atctccagcg
     8461 cgggggcatt ggcgtcatta cccaccagca ggttagcaat gcgtaacgca ggcatatcca
     8521 gtgcgccgca gtggctgata cccgactggc gaaaaccgtg acgaccgcca tcctgcacag
     8581 tggtatacat gcccgcacga ataatcttca gcatactccc tccttctgcg gtacaaagcg
     8641 cacgctgtct cccggacgta ataagatggg ttcgtcacgc gccggatcaa acaggctgag
     8701 tgaggtatga ccaatcaact gccagccccc cggcgttgcc agcggataaa caccagtctg
     8761 cggcccgccg atcccgacag aacctgccgg aacgagcaag cgcggttcag cgcgacgtgg
     8821 cgtgtgtagt tgttccggca aactcccgag atacgggaag cccggttgaa aacctaaaaa
     8881 ccagaccacg tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc tcaacccaca
     8941 atgcgccgcg accaccgcca aatccggtcc gcctgcgcca ccgtaaacca ccggaatttc
     9001 aataaaacga gactccggct ccagcgcctc actctcctcc caccagcgtt gcaaacgctc
     9061 tatggcatcc agtgccagcg actcaggatt acgtaaaatc accgtgatat tgttcatgcc
     9121 gggaatggct tcaaccacat tcggcatatc caccagacgc tgcgccagtc gccagatccg
     9181 tttctggcta gccagcgtca ccggcggttc cagctccagc actaccgccg tttcacctat
     9241 cagataacaa cgcgctcgtt gcactttcga tccctctgat tattcgttat gcaggattag
     9301 gaatatcaat aaaggtcaca tcaagatcgg tattttcatt cagccactcg ctcaatgcgc
     9361 gaataccacc gcgttcagtg gcatggtgac ccgcagcata aaaatgcaat ccctgctcgc
     9421 gggctgaatg aatggtctgt tcagaaactt cgccagtaat aaaagcatcc acgccaaaac
     9481 gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca ccaggcaacg cgctggacca
     9541 cctcaggtcc ggtatcgcca caccataacg gcttacgtcc cagacgcgct tcaatccagg
     9601 aggctagttc cagtcccggc acaggcatgg tcagttcgcc ccacggcacc aacggctcaa
     9661 tttcgcccat gaccgtgatc cccagtaacg ccgccagctg tgcgttattg cccagctcag
     9721 gatgtgcgtc aagcggcaaa tgccagccat acaggttgat atcattcgcc agcaacgttt
     9781 ttaaacggtt acgcttcatg ccgcgaatga ccggagattc gcctttccag aagtagccat
     9841 gatgcacaat gaccgcatcc gcgcccagac gcactgcctc atcgagcaaa gcctggctgg
     9901 cagtgacacc ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc aaaccattcg
     9961 gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt aatcagttgt tccagttcgg
    10021 tgtttttcat cttcgcgcct tccgtattct gatagctaca ccgttaacat agcgttacct
    10081 cgcctgttcg tcgatagcca atcttttgac atattaagaa acaaacccga cgccaaaaga
    10141 cgcaaattgt ttatatcatc acgcttcagc taatgtatat ttatgcaatc agacagcata
    10201 ttgcataaat aatcagatct gtatcaaagt tttaacttta aggaaacaca agcacgccgc
    10261 gcaactatta cgtgacgttg cagtcaattg tggaattatt atgaataaac acgcatcaca
    10321 gccgcgcgct atttactatg tcgttgcgct gcaaatctgg gaatatttta gcttttacgg
    10381 catgcgtgcc ctgctgattc tctatctcac caatcaacta aaatacaacg atactcacgc
    10441 ctacgagtta tttagcgcct actgttcgct ggtgtatgtc acgccaatcc tcggtggctt
    10501 tttggcggat aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt tgttgatggc
    10561 gatcggtcat gtggtgctgg gtgctagtga gatccatccg tcattcctct atctgtccct
    10621 ggcgattatc gtctgcggct atggcctgtt taaatctaac gtaagctgtt tgctcggcga
    10681 gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt atgcggcggg
    10741 taacgtgggg tctattatcg cacctatcgc ctgtggttac gcccaggaag agtatagctg
    10801 ggcgatgggc tttggcctgg cggcggttgg catgatcgcg ggtctggtca ttttcttatg
    10861 tggcaatcgt catttcactc atacccgcgg cgttaacaaa aaagtactgc gtgcgacaaa
    10921 ctttctcctg ccgaactggg gatggctgct ggttctgctg gtggcaacgc ctgcgctgat
    10981 taccgtattg ttctggaaag agtggtcggt atacgcctta attgtcgcga ctattattgg
    11041 cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaat cagaaacagc gtaaggagct
    11101 ggggctgatt gtgacgctca ccttcttcag tatgttgttc tgggccttcg cacagcaggg
    11161 cggcagctcg attagccttt atatcgaccg cttcgttaat cgcgatatgt ttggttatac
    11221 cgttccgact gctatgttcc agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt
    11281 cctggcgtgg gtggttaaag agagcgtcgc aggtaatcgt accgtgcgca tctggggaaa
    11341 atttgctctt ggccttggcc tgatgagcgc cggattctgc attctgacct taagcgcccg
    11401 ctggtccgca atgtatggtc actcttctct gccactaatg gtgttaggcc tggcggtgat
    11461 gggctttgcg gaactgttta tcgacccggt tgctatgtcg cagattacgc gcattgaaat
    11521 ccctggcgtg actggcgtat taactggcat ttatatgctg ctttccggcg cgattgcgaa
    11581 ctatctggct ggcgtgattg ccgatcagac atcgcaagct tcgtttgatg cttccggggc
    11641 gatcaactac tccatcaatg catatattga agtatttgat caaattacct ggggcgcact
    11701 ggcgtgtgta ggagtggtgt tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg
    11761 cgcgctggcg ctggaatctt aacaggccag caccatacct gataaaacgc tcgctgcgtt
    11821 ttatcaggct cctttcctcg cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt
    11881 gtgatcaact atcggttgcg gataatccag cgtcacacct gctttctgcg cccacttcca
    11941 cggctcatgc accgctttcc ctggtacatc gcgcagttcc ggtagccacc ggcggataaa
    12001 ctcgccttcg cggtcaaatt tctcgccctg ggttgtcggg ttaaaaatac gaaaatacgg
    12061 cgctgcatcg gttccggttg aagcggccca ctgccagcca ccgttattgg ctgccaaatc
    12121 accatcaatc agctgcgaca tgaaataccg ctcgccttcg cgccagtcga tcaataaatc
    12181 tttcaccaaa aaactggctg taatcatccg tagccggtta tgcatccagc cagtgctgtt
    12241 aagctgacgc atggcagcat cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg
    12301 taaatgtgcg ggattgcgct gccactgcac acgatccgtc cagacaataa acggacgatg
    12361 tttacacaac gagggataat acgtcatcaa atggcggtag aactcgcgcc agatcaactc
    12421 attaagccag acactaccgg ccccaccttc cagcgcctgc ggctgttccg ccaacaggcg
    12481 atgcagacac tggcgaggcg ataacccgcc cgttgccagg ctggcggaca aacggctggt
    12541 gccttctacc gcagggaaat ctcgctgttg ctcgtactcc cctgctccgc tctggcaaaa
    12601 ctggcgtaat tgcgcaatcg ccgctttctc ttccaccggg aaatgcgcag tatcgaaagg
    12661 ctgacgagga taattcagcg taatggatgg cgcgggttct atcattccgc tgctacgtac
    12721 ttttggcgca gcgacgcact ccggcatccc ttctcgcagc cgtttcagcc aggcgttttt
    12781 aaatggcgta aagactttgt acatctcatg attgccagtc atcaccgccc ccggcggcag
    12841 gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacttccac
    12901 atcccgcgcc cgctcattca cttcatactg atagttataa aaaagatgag taacgctgtt
    12961 ttccgcgcac acctgtttaa ttatttcgac gctggcggca aagtcatcca cttcacggaa
    13021 caataaagga ataccttttt ccgccagcgc tattcgtagc ccattcagtt gagcattgat
    13081 aagtttagcc tgacgcggcg acatgttatg cgtcgcccac tggcgtggtg tagcgatata
    13141 cagcgccagc acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg
    13201 cagacgtaaa tcctggcgaa accagaccag atgggtagtc ataaaactcc tgcatggcgc
    13261 aagttaatca cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga
    13321 taaagcgcca tatagtgttt gatacgcatc agcggcgcaa gaagcggttg ctcgccacgg
    13381 cgataactgt cgataagcgc cgccagctgc tggcgttctg tggaatcaat atgcgggcga
    13441 aaatacccct gaacgtgcat caaaacgtta gtatgatcgc gccggttggc agggtgagaa
    13501 agcaaaacaa ttacgcgttg gcggtactgg ttgtaatagt catccagatt ttgccactgg
    13561 tgaatatcag ccacaaacgg gccaagtttg cggtactccg gctgggaatg ggccagaaag
    13621 acgagtttat agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc
    13681 agtcgatgta actcgtggag agcgaagata cggattaggg tgctttcatc aaatcgttgc
    13741 tgattcatta ctgctcctga acggtttaca ggagtaagtg tagacggcag tattgaaaaa
    13801 acgaaacgaa taaatgagat cggtaaagaa attcaataac tcataataaa tttcatggtg
    13861 gcgataaata gtttcttttc ttcatcactc atggtagaag cgtttaacat gttgccataa
    13921 aatttactga tgatattttt taattgcagc aaatgcaaag gagaaaaata gtcactcccg
    13981 atgagatt
//
LOCUS       DAYPHN010000130        13947 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000130 DAYPHN010000000
VERSION     DAYPHN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.130"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..780)
                     /locus_tag="ACG0BW_003550"
     CDS             complement(<1..780)
                     /locus_tag="ACG0BW_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFK7260474.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVV"
     gene            993..2456
                     /gene="bepA"
                     /locus_tag="ACG0BW_003551"
     CDS             993..2456
                     /gene="bepA"
                     /locus_tag="ACG0BW_003551"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFK7260475.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            2477..2836
                     /gene="arsC"
                     /locus_tag="ACG0BW_003552"
     CDS             2477..2836
                     /gene="arsC"
                     /locus_tag="ACG0BW_003552"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFK7260476.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2942..3643)
                     /gene="hda"
                     /locus_tag="ACG0BW_003553"
     CDS             complement(2942..3643)
                     /gene="hda"
                     /locus_tag="ACG0BW_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFK7260477.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(3738..5027)
                     /gene="uraA"
                     /locus_tag="ACG0BW_003554"
     CDS             complement(3738..5027)
                     /gene="uraA"
                     /locus_tag="ACG0BW_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFK7260478.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKMISVLRPEEVVLDAEDADITDK"
     gene            complement(5113..5739)
                     /gene="upp"
                     /locus_tag="ACG0BW_003555"
     CDS             complement(5113..5739)
                     /gene="upp"
                     /locus_tag="ACG0BW_003555"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFK7260479.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6064..7101
                     /gene="purM"
                     /locus_tag="ACG0BW_003556"
     CDS             6064..7101
                     /gene="purM"
                     /locus_tag="ACG0BW_003556"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFK7260480.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7101..7739
                     /gene="purN"
                     /locus_tag="ACG0BW_003557"
     CDS             7101..7739
                     /gene="purN"
                     /locus_tag="ACG0BW_003557"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFK7260481.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            7911..9977
                     /gene="ppk1"
                     /locus_tag="ACG0BW_003558"
     CDS             7911..9977
                     /gene="ppk1"
                     /locus_tag="ACG0BW_003558"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFK7260482.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9982..11523
                     /gene="ppx"
                     /locus_tag="ACG0BW_003559"
     CDS             9982..11523
                     /gene="ppx"
                     /locus_tag="ACG0BW_003559"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFK7260483.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11562..13805)
                     /gene="pdeF"
                     /locus_tag="ACG0BW_003560"
     CDS             complement(11562..13805)
                     /gene="pdeF"
                     /locus_tag="ACG0BW_003560"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFK7260484.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITELDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQLITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         3653 a         3548 c         3502 g         3244 t
ORIGIN      
        1 caccacaaat gcaccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
       61 cagcgaatag ttcagcccga agagtaagaa tcccagccag gtggcaatcc ccaccacgat
      121 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      181 ctgtcctgcc agtccacggt tacgtggcag cacccggcga acggcgttca gcatctgctc
      241 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      301 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      361 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta acgccggata
      421 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      481 gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
      541 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
      601 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
      661 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
      721 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
      781 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
      841 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
      901 atcttcgccg ttacactcaa aggcggcgcg gtgggaacga tatttcacag tatcggtcaa
      961 atgactatct ccagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1021 ttgcaaccct cattgctgcc atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1081 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1141 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1201 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1261 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1321 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1381 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1441 tggaagatca acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1501 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     1561 gccaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     1621 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc gaccttcctc gaaaaattgc
     1681 tcgatcaggc gcgttactcc tcgcgtccgc cggaaatttt attgactcac ccgttgccgg
     1741 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgtccgatg gtggtgcagt
     1801 cgtcggagga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     1861 atcagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     1921 cagcacaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     1981 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2041 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2101 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg
     2161 gtcaaccaca agaagcggcc aatattctga atcgctacac ttttaacaat aaagatgaca
     2221 gcaatggctg ggatttactg gcacaggcgg aggcggcgct aaataaccgc gatcaggaac
     2281 tggctgcgcg agcagaaggt tatgcgctcg ccgggcgact cgatcaggcc atttccttgt
     2341 tgagtagcgc cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc
     2401 gcatcgacca attgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2461 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     2521 gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtgctcta
     2581 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     2641 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     2701 cagttcactt agcgaagagg cgctgattca ggcaatggtg gataatccga agctaatgga
     2761 gcgcccgatt gtggtcgcga atggcaaagc gcgaattggt cgtccaccgg agcaggtgct
     2821 ggaaatcgtg ggttaagaag tgctccatcg ctagttgaag cacattgccg gatgcgacgc
     2881 ttgccgcgtc ttatccggcc gacgaatact atgggtgcat ttccgacaat aaacgtcttg
     2941 tctacagttt cagaatttct ttcacaaacg gaatggtcag cttacgttgc gcggtaatcg
     3001 acgcacgatc caactgatcc aacgtcataa ataacgtgcg catttctctg tcgagccgct
     3061 tcagcaagaa acgccccaca tcttccggca gttcaaaacc acgcaaacgc gcgcgtaact
     3121 gtagcgcctg caacttatct tcatcagaaa gtggctgcaa tttgtagatc tgcccccagt
     3181 cgagacgcga cgcgagatcc ggtaatccaa gattcaactg ccgcggtgga cgatcgccgg
     3241 tgattaacaa ccgtgttttg cccgattcca gaattcgatt gtagagatcg aaaatcgcca
     3301 tctcccacaa ctcatcgcct gcaatacact caatgttgtc gatacagacc agcgacaaat
     3361 gctccatacc gtcgagcact tccggaacaa accaggtgcg tttatccagc gggacatagc
     3421 ccaccgcatc gccacgctgc gacaattccg cgcaagccgc gtgcagcaga tggctgcgcc
     3481 ccgcgccttc gcgtgcccag agatagatgt aaccgctatg ttcctgacgc agcacgtttt
     3541 gcagcgcggc cagtaaagag gagttatccc ccggccagaa acttgcaaag gtttcgtcgt
     3601 caggaagata aagtggcaaa gagagctgtg ccggtgtgtt cagagatacc tcaaccagga
     3661 tttcacaaaa tcgcgagaag tttaccacag aatcccatga tgtttgaacc gggcagcaac
     3721 actgcccggt cggtacatta tttgtctgtt atatccgcgt cttctgcgtc cagcaccact
     3781 tcttcagggc gcagcacact aatcattttg aagatcaaac ttaagccgat accgacgatg
     3841 gtcgccagcg ccataccttt cagctccgcc gcgccgatgt ttaccttcgc gccactgaca
     3901 ccgatgatca aaatcacgga agtcaggatc aggttctgtg ctttgttgta atccactttc
     3961 gattcgatca aaacacgaat accggaagca ccgatgacac cataaagcag cagcgaaacg
     4021 ccgcccatca ccggcaatgg gatcatctgg atagcagcag ccagtttacc gacgcaggaa
     4081 agcaggatag cgaaaatcgc cgccccgccg ataacccagg tactgtaaac acgggtgatc
     4141 gccatcacgc caatgttttc cccgtaggtg gtgtttggcg tggagccaaa gaagccggag
     4201 atcacggtcg acaagccgtt agcaaacatc gaacggtgca gacctggatc gcgcagcaga
     4261 tcttttttga cgatattagc cgttaccacc aggtgcccta cgtgttcggc aataaccact
     4321 aacgccgctg gcagaatagt cagaatggca aaccactcga agcgcggcgt atagagggtt
     4381 ggcagcgcaa accagtgagc attaataatc ggcgtggtat cgacaattcc cattgcgaaa
     4441 gagagcgcgt aacccaccag cacgccaatt aaaatcggga taattgccag gaaaccacga
     4501 aacagcacgg aacctaaaac cgtgactgcc agggtggtaa tggagatgat gatggttttg
     4561 gagtctggcg tttgcccttc agccgggagt aaacctgcca taccggcagc tacgcccgcc
     4621 agctccagac cgatgacggc aacgattgcg cccattgccg caggtggaaa cagcacgtcc
     4681 agccagccgg tccccgcttt cttcacgata aaagaaacca ggcagaacag cacgccgcac
     4741 ataataaagc cgcccagcgc gacttcatac cctaacggca acagtaacaa taccggtgaa
     4801 ataaaggcaa agctggaacc aagataagcc gggattttcc ctttacagat gaagagatac
     4861 agcagcgttc ctataccgtt aaataacagt acagtcgccg ggttaatatg aaataagacg
     4921 ggcaccagga cggttgcacc aaacatggcg aacaaatgtt gcaaactaag cgggattgtc
     4981 tgtaaaagtg gcggtctttc actcaccccg atagcacggc gcgtcatagt attatcctct
     5041 gtattatgtg ttataggcgc tttactcaaa aaaaagccga ctcttaaagt cggctttaat
     5101 tatttttatt ctttatttcg taccaaagat tttgtcaccg gcatcgccga ggcccggaat
     5161 aatgtatccg tgctcgttca gtccctggtc aattgatgca gtatacagtt cgacgtccgg
     5221 gtgcgctttt tccagcgctg cgataccttc tggtgcagct accagcacca gaactttgat
     5281 gctgctgcag cccgcttttt tcagcaggtc gatggtcgcg ataacggaac caccggttgc
     5341 cagcattggg tcaacgatca gcgccatacg ctcatcgatg ttagaaacca gtttctggaa
     5401 gtacggtacc ggctccagcg tttcttcatt acggtacata ccgacgacgc tgatgcgcgc
     5461 gctcggaacg ttttccagca caccgtccat catccccaga cccgcacgca gaattggcac
     5521 aacggtaatt ttcttacctt tgatctggtc gatttctacc gggccgttcc agccttcgat
     5581 agttactttt tccgtttcga ggtcggcggt cgcttcgtaa gtcagcaggc tacccacttc
     5641 ggaagcgagt tcgcgaaagc gcttggtgct gatatcttgc tcacgcatca gtcccagctt
     5701 gtgtttgacg agtgggtgtt tgacttccac gatcttcata ctctttctcc tttgaggggc
     5761 agccacaaaa aaaatcgacg gattatacct cctttcttca agccggcaat attattttcg
     5821 ttgactttag tcaaaatgat aacggtttga gataaagtta ttttgtattc aaatggttat
     5881 ggaagaagat tattccatcc gaaaacgatc ctttgccctg gtacaagtat tctttcgccg
     5941 cgcgcctggg gaaaagacgt gcaaaaaggt tgtgtaaagc agtctcgcaa acgtttgctt
     6001 tccctgttag aattgcgccg aattttattt ttctaccgca agtaacgcgt ggggacccaa
     6061 gcagtgaccg ataaaacctc tcttagctac aaagatgccg gtgttgatat tgacgcgggt
     6121 aatgctctgg ttggaagaat caaaggcgta gtgaagaaaa cgcgtcgtcc ggaagtgatg
     6181 ggcggtctgg gcggcttcgg tgcgctgtgt gcattgccgc aaaaatatcg tgaacccgtg
     6241 ctggtttccg gcactgacgg cgtaggtacc aagctgcgtc tggcgatgga cttaaaacgt
     6301 cacgacacca ttggtattga tctggtcgcc atgtgcgtta atgacctggt ggtgcaaggt
     6361 gcggaaccgc tgtttttcct cgactattac gcaaccggaa aactggatgt tgataccgcg
     6421 tcagcggtga tcagcggcat tgcggaaggt tgtctgcaat cgggctgttc actggtgggt
     6481 ggcgaaacgg cagaaatgcc ggggatgtat cacggtgaag attacgatgt cgctggtttc
     6541 tgcgttggcg tggtagaaaa atcagaaatc atcgacggct ctaaagtcag cgatggcgat
     6601 gtgctgattg cactcggctc cagcggtccg cactcgaacg gctattcgct ggtgcgcaaa
     6661 attcttgaag tcagcggttg tgatccgcaa accaccgaac ttgatggtaa gccattagcc
     6721 gatcatctgc tggcaccaac ccgcatttac gtgaagtccg tgctggagtt gattgaaaag
     6781 gtcgatgtgc atgctattgc gcacctgacc ggcggcggct tctgggaaaa cattccgcgc
     6841 gtattgccag ataatactca ggcagtgatt gatgaatcat cctggcagtg gccggaagtg
     6901 ttcaactggc tgcaaacggc aggtaacgtt gagcgccatg aaatgtatcg caccttcaac
     6961 tgcggcgtcg ggatgattat tgccctgcct gctccggaag tggacaaagc cctcgccctg
     7021 ctcaatgcca acggtgaaaa cgcgtggaaa atcggtatca tcaaagcctc tgattccgaa
     7081 caacgcgtgg ttatcgaata atgaatattg tggtgcttat ttccggcaac ggaagtaatt
     7141 tgcaggcaat tattgacgcc tgcaaaacca ataaaattaa aggcaccgta cgggcagttt
     7201 tcagcaataa ggccgacgcg ttcggccttg aacgcgcccg ccaggcgggt attgcaacgc
     7261 atacgctcat cgccagcgcg tttgacagtc gtgaagccta tgaccgggag ttgattcatg
     7321 aaatcgacat gtacgcaccc gatgtggtcg tgctggctgg ttttatgcgc attctcagcc
     7381 cggcgtttgt ctcccactat gccgggcgtt tgctgaacat tcacccttct ctgctgccga
     7441 aatatcccgg attacacacc catcgtcagg cgctggaaaa tggcgatgaa gagcacggta
     7501 catcggtgca tttcgtcacc gatgaactgg acggtggccc ggttatttta caggcgaaag
     7561 tcccggtatt tgctggtgat acggaagatg atatcaccgc ccgcgtgcaa acccaggaac
     7621 acgctattta tccactggtg attagctggt ttgccgatgg tcgtctgaaa atgcacgaaa
     7681 acgccgcgtg gctggatggt caacgtctgc cgccgcaggg ctacgctgcc gacgagtaat
     7741 gcccccgtag ttaaagcgcc agctctgccg ctggcgtttt tcaattcacc tgtaaatcgc
     7801 aagctccagc agtttttttc ccccttttct ggcatagttg gacatctgcc aatattgctc
     7861 gccataatat ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta atgggtcagg
     7921 aaaagctata catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg
     7981 aagcggcgga caaatctaac ccgctgattg aaaggatgcg tttcctgggg atctattcca
     8041 ataaccttga tgagttctat aaagtccgct tcgctgaact gaagcgacgc atcattatta
     8101 gcgaagaaca aggttccaac tctcattccc gccatttact gggcaaaatt cagtcccggg
     8161 tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga gctgttgctg gagatggcgc
     8221 gcaaccagat cttcctgatt aatgaacgcc aactctccgt caatcaacaa aactggctgc
     8281 gtcattattt taagcagtat ctgcgtcagc acattacgcc gattttaatc aatcctgaca
     8341 cagacttagt gcagttcctg aaagatgatt acacctatct tgcggtggaa attatccgtg
     8401 gcgataccat ccgttacgcg ctgctggaga tcccatcaga taaagtgcca cgctttgtga
     8461 atttaccgcc agaagcgccg cgtcgacgca agccgatgat tcttctggat aacattctgc
     8521 gttattgcct tgatgatatt ttcaaaggct tctttgatta tgacgcgctg aatgcctatt
     8581 caatgaagat gacccgcgat gccgaatacg atttagtgca tgagatggaa gccagcctga
     8641 tggagttgat gtcttccagt ctcaagcagc gtttaactgc tgagccggtg cgttttgttt
     8701 atcagcgcga tatgcccaat gcgctggttg aagtgttacg cgaaaaactg actatttccc
     8761 gctacgactc catcgtccct ggtggtcgtt atcataattt taaagacttt attaatttcc
     8821 ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc gcgtttacgc catatttggt
     8881 ttgataaagc ccagttccgc aatggttttg atgccattcg cgaacgcgat gtgttgctct
     8941 attatcctta tcacaccttt gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc
     9001 cgagcgtact ggcgattaaa attaacattt accgcgtggc gaaagattca cgcatcatcg
     9061 actcgatgat ccacgccgca cacaacggta agaaagtcac cgtggtggtt gagttacagg
     9121 cgcgttttga cgaagaagcc aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc
     9181 acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg atttcacgta
     9241 aagaaaacgg tgaagtggtg cgttacgcac acatcgggac cggaaatttt aacgaaaaaa
     9301 ccgcgcgtct ttatactgat tattcgttgc tgaccgctga tgcgcgcatc accaacgaag
     9361 tacggcgggt gttcaacttt attgaaaacc cataccgtcc ggtgacattt gattatttaa
     9421 tggtgtcgcc gcaaaactcc cgccgcctgt tgtatgaaat ggtggaccgc gaaatcgcca
     9481 acgcgcagca agggctgccc agcggtatca ccctgaagct aaataacctt gtcgataaag
     9541 gcctggttga tcgtctgtat gcggcctcca gctccggcgt accggttaat ctgctggttc
     9601 gcggcatgtg ttcgctgatc cccaatctgg aaggcattag cgacaacatt cgtgccatca
     9661 gtattgttga ccgttacctt gaacatgacc gggtttatat ttttgaaaat ggcggcgata
     9721 aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa tattgattat cgtattgaag
     9781 tggcgacacc gctgctcgat ccgcgcctga agcagcgggt gctggacatc atcgacatat
     9841 tgttcagcga tacggtcaaa gcacgttata tcgataaaga actcagtaat cgctacgttc
     9901 cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat ttacgactac atcaaatcac
     9961 tcgaacaacc tgaataaccc tatgccaata cacgataaat cccctcgtcc gcaggagttt
    10021 gctgcggtcg atcttggttc aaacagtttt cacatggtca tagcccgtgt ggtagatggt
    10081 gccatgcaga ttattggccg cctgaaacag cgggtgcatc tagcggacgg cctggggcca
    10141 gataatatgt tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc
    10201 gaacggctac aaggtttttc tcctgccagc gtctgtatag tcggcaccca tacgctgcgt
    10261 caggcgctga acgccactga ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt
    10321 gaaattattt ccggtaatga agaagcccgt ctgattttta tgggcgtgga acatacccaa
    10381 ccggagaaag gtcgcaaact ggttattgat attggcggcg gttctacaga actggtgatt
    10441 ggggaaaatt tcgaaccgat tctcgttgaa agccgccgga tgggttgtgt cagctttgcc
    10501 cagctttact tccctggcgg ggtcatcaat aaagagaatt ttcagcgcgc tcgtatggcg
    10561 gcagcacaaa aactggaaac tttaacctgg caattccgta ttcagggctg gaacgtggca
    10621 atgggcgctt ccggtaccat aaaagccgcc catgaagtgt taatggaaat gggcgagaaa
    10681 gacgggataa ttaccccgga acgtctggaa aaactggtaa aagaagtttt acgtcaccgt
    10741 aatttcgcat cgctgagttt accgggtctt tctgaagagc ggaaaacagt cttcgttcct
    10801 ggactggcga ttttatgcgg tgtgtttgat gctttagcca tccgtgaact gcgcctttct
    10861 gacggggcgt tacgcgaagg cgtactgtat gaaatggaag gacgtttccg tcatcaggat
    10921 gtgcgtagtc gcaccgccag cagcctcgcc aaccagtatc acatcgacag cgaacaggcc
    10981 cgacgggtgc tggataccac tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag
    11041 ctggcgcatc cgcaactgga ggcgctactg cgatgggccg ccatgctgca tgaggtcggg
    11101 ttgaatatca accacagcgg tttgcatcgc cactccgctt atattctgca aaacagcgac
    11161 ttgccgggtt ttaatcagga acagcagttg atgatggcga cgctggtgcg ctatcaccgt
    11221 aaagcgatta agctcgacga tctgccgcgc tttaccttgt ttaagaagaa acagttcctg
    11281 ccactgattc agctattgcg ccttggcgta ttactcaata atcaacgtca ggcaaccacc
    11341 acaccgccaa cattgacgct gattaccgat gacagtcact ggacactgcg ttttccgcat
    11401 gactggttta gtcagaatgc gctggtactg cttgatctgg aaaaggagca agaatactgg
    11461 gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa gtacaccaga aatcgccgct
    11521 taatgctttg caggccgaca agaaatgtcg gcctgcatta ttcaggcact ttcgcgaatg
    11581 ggttcgattt cattcagcgt atcaattaac ggttgcggct taccaataag ataaccctgc
    11641 atataatcga tccccaaaga gagcaccgcc tcgcggatct cttcgttttc aacgtactct
    11701 gccactaccc gcattttctt cattcgcgcc aggtggcaaa tcgacgccac tatctgataa
    11761 tccagactat ttgacacaat attgcggata aaactgccgt caattttaag caaatcggca
    11821 ttcacatttt taagccgcgc atagctggcg tagccggtgc cgaaatcatc aatcgcaatc
    11881 tggcagccta attcctgaag atgctgcaag gtgatttgcg cctgcttaac attggtcaga
    11941 gcattacttt cggtgacttc aaaaataagt tgccacgctt caatttgata tttagccagc
    12001 agctgactga cttcaacggg aaaacgagcc tgacataccg aggttggcga cagattaatc
    12061 gcaaaacggt gagcgggcat cttcgctctg ttttcagcca taaattgcag cgtatgctca
    12121 atgacccaca tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg caagaagctg
    12181 tcggggctga tcagttcatc attctcacct ttcatgcgca gaagaatttc atggtagaca
    12241 tcgccacgca taccggtaat cagctgggcc atcaggaaaa aatggttgtg ttccagcgcc
    12301 tgctgtagcc gattcatcat cgcgacttta tctttcaatt cgcgttgcaa atacattgcc
    12361 ccgcgacgct gcatattttc cggggcgttg gtcacgatgg aaagctcggc gaccgtattt
    12421 agctctccca gcagcaggta gatatgattc actggcgagc gcacatagca gtaactgacg
    12481 ccaatctgcg gttgcatcgg cattccatcc caaaagaaac gaaattgctt gagatggcta
    12541 tccagttcgg taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta
    12601 cccgaaagct gataaacatc ctcacccggt tccagcaagg gtgacagcca gtgagaaagt
    12661 ttttgcttgt attgaatccg cagcatgatg ccatagttct taaccagcat ttccatgcca
    12721 gggatgcgta aataacaaag cgcagaccag ggggcatcac gcaacgcgcg attcagggcg
    12781 cgaacatttg gcagatgaac caccgggtcc acatacgcaa gccgctgaat gcgtctgact
    12841 accgctctct gacgggttgc cagtacagcc atataattga caataaaaga gaataccaga
    12901 taactggagg aggttatggt cagctgcgtg gtatagccag gataaatggg aatgtaattt
    12961 tgatagctgt ggatgctgat catcaacacg accgcccaga gcagcgaaat cagcttataa
    13021 ccatagcgca tcgctcccca catcatcagg ggcagcaata atgacaaggt atagttggtg
    13081 ctaaaaattg tgcttttttc atttaacggc atgcataaca gcagcagtaa agcacctaat
    13141 gccagtagcc agagtgcgaa ctcttttttg gtgactttga catcaacctg ctgttttaat
    13201 tgcgaataat aactacgtaa ataaaacgga tttcgcacta cacgaatgat gaagtagcac
    13261 agcgggacac caatcagatt gcccaccagt aaggcctgat aattgattaa ggtcccgagg
    13321 ttaaaaggca tgacgccgac cagattttct ctgctcgcca gtaatcctac aaacgcagca
    13381 aactggaaaa gtatcagaaa cagcgttgca ggaaacacaa tctgccagaa tatacgctgg
    13441 gaaattaaac gtgtatcacc atgtgaaacg ttgttacgcc ggggagtaaa gaccctgtaa
    13501 ccgccccaac agagaatgat aatgataata aaattagccg ttaatgagag cgtttcgtaa
    13561 aaacccagct ctgcatattt gcggacaaaa atccctaagg cgatccctgg caatgccgcc
    13621 cagctgaaaa acatcatcat gctgatcatc agagccagag gcagataaaa gagaaaaacc
    13681 tcaccggaag aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa gattaaagaa
    13741 ggcaggaaca gcggaagccc ccaccattta tcacgtattt ttatataagt tgcattcagt
    13801 ttcatagatg ctcagcagaa tcccccacat cctgaaggag gtgtattcag acaggcatcc
    13861 cacctgactt cgaatgatga ttattcatca ctatagagag cattgattct aagtgtcata
    13921 tgaaagttcc aattgatata tatcaaa
//
LOCUS       DAYPHN010000131        13888 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000131 DAYPHN010000000
VERSION     DAYPHN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.131"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            89..331
                     /gene="yjbE"
                     /locus_tag="ACG0BW_003561"
     CDS             89..331
                     /gene="yjbE"
                     /locus_tag="ACG0BW_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFK7260485.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            445..1083
                     /locus_tag="ACG0BW_003562"
     CDS             445..1083
                     /locus_tag="ACG0BW_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFK7260486.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     DVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEDVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1080..1817
                     /locus_tag="ACG0BW_003563"
     CDS             1080..1817
                     /locus_tag="ACG0BW_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFK7260487.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1817..3913
                     /locus_tag="ACG0BW_003564"
     CDS             1817..3913
                     /locus_tag="ACG0BW_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFK7260488.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3960..4238)
                     /locus_tag="ACG0BW_003565"
     CDS             complement(3960..4238)
                     /locus_tag="ACG0BW_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260489.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKTDLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4452..4862
                     /gene="psiE"
                     /locus_tag="ACG0BW_003566"
     CDS             4452..4862
                     /gene="psiE"
                     /locus_tag="ACG0BW_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFK7260490.1"
                     /translation="MTSLSRPCVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4956..5846)
                     /gene="malG"
                     /locus_tag="ACG0BW_003567"
     CDS             complement(4956..5846)
                     /gene="malG"
                     /locus_tag="ACG0BW_003567"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFK7260491.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5861..7405)
                     /gene="malF"
                     /locus_tag="ACG0BW_003568"
     CDS             complement(5861..7405)
                     /gene="malF"
                     /locus_tag="ACG0BW_003568"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFK7260492.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7559..8749)
                     /gene="malE"
                     /locus_tag="ACG0BW_003569"
     CDS             complement(7559..8749)
                     /gene="malE"
                     /locus_tag="ACG0BW_003569"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFK7260493.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGHPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9114..10229
                     /gene="malK"
                     /locus_tag="ACG0BW_003570"
     CDS             9114..10229
                     /gene="malK"
                     /locus_tag="ACG0BW_003570"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFK7260494.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10301..11641
                     /gene="lamB"
                     /locus_tag="ACG0BW_003571"
     CDS             10301..11641
                     /gene="lamB"
                     /locus_tag="ACG0BW_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFK7260495.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11791..12711
                     /gene="malM"
                     /locus_tag="ACG0BW_003572"
     CDS             11791..12711
                     /gene="malM"
                     /locus_tag="ACG0BW_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFK7260496.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            12940..>13888
                     /locus_tag="ACG0BW_003573"
     CDS             12940..>13888
                     /locus_tag="ACG0BW_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFK7260497.1"
                     /translation="MLGHISKFDGNNSLIKHDVVQGNNKLDLDLLRNFNGVPGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMFREVSISNDIISVKFYRNEEIECACDFLMDKDAQG
                     YTDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGDCNFTTATVDNVIFKCRR
                     LHNVIFIKASGEYVDFSQSILDTVDFSRSQLTHSNFRECQIRNSKFNNCYLYASHFTR
                     AEFLSDKEISFIKSNLTAVVFDHVRISTGNFKDSVTQRMVLSIDYSDIFGNEYLDGYI
                     NNIIKMIDTLPDDPAILKSVLAVKLVMQLKILNIVNKNFI"
BASE COUNT         3440 a         3486 c         3623 g         3339 t
ORIGIN      
        1 gtaattcgca tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc
       61 tccattcatt caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat
      121 atctgcgctt gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg
      181 gtcggcagca acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag
      241 ctcggcggtg ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac
      301 cacaacaacc acgaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag
      361 tcattgcatc tgtggcttga agtatgaagg gatttaccat aaccacactc cggtgtggtt
      421 attctgcccc tctggagaag agtcgtgaag cgacctgcac tcattcttat ctgcctgcta
      481 ttacaggcct gttcagccac gactaaagag ctgggcaatt cgctgtggga cagtctgttc
      541 ggcacgccag gcgtacagct gacggacgat gatattcaaa atatgcccta cgccagccag
      601 tacatgcagc ttaatggcgg gccgcaattg tttgtggtgc tggcctttgc tgaagacgga
      661 caacaaaaat gggtcactca ggatcaggcc actctcgtca cacaacatgg tcgtctggtg
      721 aagactttgc ttggcggcga caacctgatt gacgtgaata accttgccgc cgacccgctg
      781 attaaacccg cacaaattgt tgatggtgca acatggacgc gcacgatggg ctggaccgag
      841 taccagcagg tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccgatacc
      901 gtcaaagtcg gcagcgatga aactccggtt cgcgtgctgg acgaagacgt ctccaccgac
      961 caggcgcgct ggcataaccg ctattggatc gacagcgaag ggcaaattcg ccagtcggaa
     1021 cagtatctcg gcgcggatta tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa
     1081 tgattaaaca aactattgtc gcattgattt tgagtgtggg ggcgtcatcg gtcttcgcgg
     1141 cgggaaccgt caaggtattc agcaatggca gcagtgaggc caaaacgctg acgggggcag
     1201 agcatttaat cgatctggta ggccaaccgc ggctggcaaa cagttggtgg cccggtgcgg
     1261 tgattagcga agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac
     1321 ggctggcaga acagggcgca gattccagcg ccgacgatgc cgctgcgatt aacgccttac
     1381 gccagcaaat tcaggcgttg aaagtgacgg gcaggcaaaa aatcaatctt gatcccgata
     1441 tcgtccgcgt tgccgaacgc ggtaacccgc cgttgcaggg caactacacg ctgtgggtcg
     1501 gaccaccgcc gtccacggtc acgttgttcg ggcttatcag ccgtcctggc aagcagccat
     1561 tcactccagg tcgcgacgtg gcgagctatc tctccgggca gaacctgctt agcggtgcgg
     1621 atcgcagcta cgcgtgggtg gtttacccgg acggacgcac gcaaaaagcg ccggtggctt
     1681 actggaacaa gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg
     1741 actccgtctg gagtgagacg cctgatgccc ttaacgccga cattcttcag actctgacgc
     1801 agcggatacc tcaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag
     1861 cacagcttgc tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg
     1921 tggcgtagga ttattacaaa cgcccaccgc acgtatggca cgggaagggg agctgagcct
     1981 gaactatcgc gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg
     2041 gctggaaaca acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc
     2101 attctctggc gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga
     2161 agagagttac tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct
     2221 gtttgatgcg gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgctcgg
     2281 cctgggatgg ggatatctgg gcaccagcgg taatgtgaaa aatccgctct gttcagccag
     2341 tgataaatat tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca
     2401 gatgttccac ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc
     2461 gctgcgcctg aaactggagt atgaaggtaa taattatcag caggattttg ccgggaagct
     2521 ggagcaaaaa agtaagttta acgtcggggc gatttatcgc gttaccgatt gggccgacgt
     2581 taaccttagc tatgaacgtg gcaacacctt tatgtttggc gttacgttgc gcaccaactt
     2641 taacgatctg cgcccgtctt acaacgataa cgcccgcccg caatatcaac cgcagccgca
     2701 ggatgccatt ttgcagcatt cggtggtggc gaatcagtta acgctgttga aatacaatgc
     2761 cggacttgcc gatccacaga tccaggcgaa aggcgatacg ctgtatgtca ccggcgagca
     2821 ggtgaaatat cgtgattcgc gtgaagggat catccgtgct aatcggatcg tgatgaacga
     2881 tctgccggat gggatcaaaa cgatccgcat tacggaaaat cgccttaaca tgccgcaggt
     2941 gacgacggaa accgatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca
     3001 cgaaacgacg ctggcgcaaa aacgcgtcga gccagtggtt ccgcagtcca ccgagcaggg
     3061 ctggtatatc gacaaatcac gctttgattt ccatatcgat ccggtgctga accagtcggt
     3121 cggtggcccg gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg
     3181 gctgacggac catctgctga ccactggaag cctttttgca aatcttgcca acaactacga
     3241 caagtttaac tacactaatc caccgcagga ctcgcactta ccgcgcgttc gtacccatgt
     3301 gcgcgagtat gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca
     3361 tctgggtaac ggcttctacg gccaggtcta cggaggttat ctcgaaacca tgtttggcgg
     3421 tgcgggggca gaagtgttgt atcgcccgct ggacagcaac tgggcgtttg gtgtggatgc
     3481 caactacgtt aaacagcgcg actggcgtag tgcaaaagat atgatgaaat tcaccgacta
     3541 cagcgtgaaa accggacatc tgaccgccta ctggacgcca tctttcgctc aggatgtgtt
     3601 agttaaagcc agcgtcgggc agtatctggc tggggataaa ggcggcacgc tggagatcgc
     3661 caaacgcttt gatagcggcg tggtggtggg tggctatgcc acgatcacga atgtttcgaa
     3721 agaggagtac ggcgaagggg acttcaccaa aggcgtgtat gtctctgtac cgctggatct
     3781 cttctcgtct ggcccgacac gcagccgtgc ggcaattggc tggacgccgc tgacgcgtga
     3841 cggtggtcag caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt
     3901 caatttccgc taagtcatgg aaaaggtgcc agttttcgca ctcactggca ccctcattct
     3961 taatggcctg tcgagccatc aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa
     4021 aggcgaaaac attctgtctt cctgtccgtc gatattggtt gcaggtctgg ttgcctgagc
     4081 gactgggcca tcattgggct ggagacattg ctttgcggta ggtcggtttt atcagcagta
     4141 ccgagcggac cagcataagc aggaagagca gagactgata acatcaaagc agcaaaataa
     4201 ggcttcattt ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg
     4261 atttttcgcg cattgctggt ggctgggaat cacctgaatg ggtgattttt tggacagtgt
     4321 gttttgccag atacgatgtt gatgggagat gccacttaat ccaggttgaa caaaacatac
     4381 acaaaaaata tagatctccg tcacattttt gcgttataca ggaagctcgc cactgtgaag
     4441 gaggtactgc tatgacgtca ctctctcgtc cgtgcgtgga gtttatctcc accattttgc
     4501 agaccgtact caatcttggc ctgttgtgcc tcggcctgat tttggttgtc ttcctcggca
     4561 aagaaacggt acatctggct gatgtgctgt tcgcgccaga acaaaccagt aaatatgagc
     4621 tggtagaagg gctggtggtt tactttctct atttcgaatt tatcgcgctg attgtgaaat
     4681 actttcagtc cggttttcac ttcccgctac gttactttgt ctatattggg atcaccgcca
     4741 ttgtgcgctt gatcatcgtc gatcataaat cgccactgga tgtgttgatc tactcggcag
     4801 cgatcctgtt gctggtgatc accctgtggc tgtgtaattc gaaacggctg aagcgggagt
     4861 aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa gttgggataa cgtaagttga
     4921 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
     4981 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taaagcagac
     5041 atcacggcag cggcagcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
     5101 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
     5161 tcagtaatgg cagcgataaa cgacaggatg aacaccaccg ccagaatcgg tactgacagc
     5221 ggcaacagga caaggcgaaa ggcctgccac ggtgtcgcac catccagcgc cgccgcttct
     5281 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
     5341 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
     5401 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
     5461 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
     5521 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc ggcgaccttt
     5581 accgagttcc acagccatag cagtaccgga aacggcggcg gtgtaacgcg accatcagcc
     5641 tgttccacgc taaaacctaa tgccagtttc cagtgatccc aggagatttg ctccgggatc
     5701 aggctgccgg tcgcaaagtt cccctggcgc agtgagatag cgacgaccat cagcagcggg
     5761 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
     5821 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
     5881 tggctttcag gttcactatc gccagcgcac ccaccagcag gaagatcagc gtggcaattg
     5941 ctgccgccag accgaagtcc tgacccccgc cgccttcaaa agcgatgcgg taggtgtagt
     6001 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc ggaccgccgt
     6061 tggttaacag ttgaatcagc acgaagttgt taaagttaaa ggcaaagctg gcgatcatca
     6121 gcggcgtcag cggtttaatc agcagcggta gcgtaatctt aaagaagttc tggaatggac
     6181 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
     6241 tgcagaggat catcatgtac ggataaccca gccaggtgtt gacgataatc agcatcgtgc
     6301 gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgca ctcaacatca
     6361 tgttgatctc accgaagctc tggttaaaca accctttgaa aatcaagatt gaaatgaacg
     6421 atggcaccgc atagggcaga atcagcagga cgcgatagac cgctttgccg cgcaacgctt
     6481 cccactgcac cagacacgcc agaaccatgc cgaccgccac cgttaaaaag acagtgatca
     6541 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
     6601 tgaagacgcg ggtaaagttt ttccagccgg tggtcacggt ataaccgggg cttagctttt
     6661 catcgcccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
     6721 gatatttcac accgctctga ttattcgtca acgtaccgtc accgtcgagt gtgtagagcg
     6781 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tccggcagaa
     6841 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcggaga ttcgcgcgtt
     6901 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
     6961 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
     7021 cgcccgccgg gtaaagacca aaattatagg ttttgcctgc ttgccaggag cgatctaaca
     7081 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
     7141 tggcgatggt gcagaccaaa gggaagagga cgaataatcc cattccagcc attcccgggt
     7201 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
     7261 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
     7321 aacccaccag caggccgagc agacctagca ctgaccattt cagcgcgtcg ctttgccacc
     7381 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
     7441 cacgccttat ccgacaacaa ctgcctgatg cgacactgat gcgtcttatc aggcctacat
     7501 gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc atccggcatt tcacagcatt
     7561 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
     7621 cggcgttgat caccgcagta cgtacggcat accagaaagc ggacatctgc gggatgtttg
     7681 gcatgatttc acctttctgg gcgttttcca tggtggcagc aatgcgtgga tctttcgcca
     7741 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttatcttta ttaaccgctt
     7801 ccagaccttc gtcagtcagc agatagtttt caaggaactc tttcgccagc tctttgttcg
     7861 gactggcggc gttaatacct gcgctcagca cgccaacgaa cggtttggat ggatgaccct
     7921 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
     7981 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
     8041 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
     8101 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
     8161 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
     8221 ggttgaacat cagcgcgctc ttgcctttcg ctttcagttc tttatccagc gccgggatct
     8281 cttcccaggt ttttggcggg ttcggcagca gatctttgtt ataaatcagc gataacgctt
     8341 caacagcgat cgggtaagca atcagcttgc cgttgtaacg tacggcatcc caggtaaacg
     8401 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
     8461 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
     8521 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
     8581 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
     8641 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
     8701 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
     8761 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacagcaac
     8821 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
     8881 attggcggta atgtggagat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
     8941 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
     9001 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
     9061 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
     9121 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
     9181 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
     9241 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
     9301 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
     9361 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
     9421 caggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctacaac
     9481 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgtgtgg
     9541 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
     9601 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
     9661 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
     9721 aaatcgtggt gctggacgct ggtcgcgtgg cgcaggttgg gaaaccgctg gagctgtacc
     9781 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
     9841 cggtaaaagt gaccgccact gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
     9901 gtcagcaagt ctggctgccg gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
     9961 tgggtattcg cccggaacat ctactgccaa gtgatatcgc tgacgtcatc cttgagggtg
    10021 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ccatatccag atcccttcca
    10081 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    10141 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    10201 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    10261 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    10321 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    10381 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    10441 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    10501 tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat aagagcttct atttcgacac
    10561 taacgtggcc tattccgtcg cacaacagaa tgactgggaa gctaccgatc cggccttccg
    10621 tgaagcaaac gtgcagggta aaaacctgat cgaatggctg ccaggctcca ccatctgggc
    10681 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    10741 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    10801 agcaacccgc tcctctgaag caggtggttc ttcctctttc gccagcaaca atatttatga
    10861 ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa
    10921 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    10981 tcgtctggtt gatggcgcat cgaaagacgg ctggttattc actgctgaac atactcagag
    11041 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    11101 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    11161 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggagcgatct ccatgggcga
    11221 caactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    11281 caccaagtgg tggaccgtcg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    11341 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    11401 gtacaaaatt acccttgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    11461 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttatg actacaacgg
    11521 cgatagcaag gttaacccga actacggcaa agccgttcct gctgatttca acggcggcag
    11581 cttcggccgt ggtgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    11641 atagcaaaac ctgggccgga taaggcgttt acgccgcatc cggcataaaa caggttgtca
    11701 ttatctgaaa ggggcgaaag cccctctgat tatcgggttt agcgcgctat tgcctggcta
    11761 ccgctgagct ccagattttg aggtgaaaca atgaaaatga ataaaagtct catcgccctc
    11821 tgtttatcag cagggttgct ggcaagcgcg cctggaatca gccttgccga tgttaactac
    11881 gtaccgcaaa acaccagcga cgcgccagcc attccatctg ccgcgctgca acaactcacc
    11941 tggacaccgg tcgatcaatc taaaacccag accacccaac tggcgactgg cggccaacaa
    12001 ctgaacgttc ccggcatcag tggtccggtt gctgcgtaca gcgtcccggc aaacattggc
    12061 gaactgaccc taacgctgac cagcgaagtg aacaaacaaa ctagcgtatt tgcgccgaac
    12121 gtgctgattc ttgatcagaa tatgacccca tcagccttct tccccagcag ttatttcacc
    12181 taccaggaac caggcgtgat gagtgcagat cgtctggaag gcgttatgcg cctgacaccg
    12241 gcgttggggc agcaaaaact ttatgttctg gtctttacca cggaaaaaga tctccagcag
    12301 acgacccaac tgctcgaccc ggctaaagcc tatgccaaag gcgtcggtaa ctcgatcccg
    12361 gatattcctg acccggttgc tcgtcatacc accgatggct tactgaaact gaaagtcaaa
    12421 acgaactcca gttccagcgt gttggtcggg ccactatttg gttcttccgc tccagctccg
    12481 gttacggtag gcaacacggc ggcaccagct gtggctgcac ccgctccggc accggtgaag
    12541 aaaagcgagc cgatgctcaa tgacacggaa agttatttta ataccgcgat caaaaacgct
    12601 gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg atgaagctga acgcctgggg
    12661 tcaacatctg cccgttccac ctttatcagc agtgtaaaag gcaaggggta attacgcccc
    12721 acagtgctga ttttgcaaca actggtgcgt ctcctggcgc accttttttt atgcttcatt
    12781 ccagggatat gagcgatttt ttatagtact tcacttcttc ttcactaaga atatccatta
    12841 tctcaatgca ttatcagaga ttcttttcct ttcgccggta gtgtctggac gctcaggcta
    12901 ctttttccag ttatttcatt tctgtaatgc agaggtttta tgttaggtca tatctcaaag
    12961 tttgacggca ataactcttt gataaaacat gatgtggtgc aaggaaataa taaattagat
    13021 cttgatttac tacgtaattt taatggggtg ccaggtttaa atagagataa ctttatttat
    13081 attagtgata tatttttaaa tataaaacaa cggaacgaaa aaaatcatgc aataaatatg
    13141 tttcgtgaag tctcaatcag taatgatatt ataagcgtaa aattctatag aaatgaagaa
    13201 atagaatgtg cttgtgattt tctgatggat aaagatgcgc aggggtatac tgacctgtct
    13261 gatttggatt taacaagttg tcattttaaa ggtgacgtta tttcgaaggt gtctttttta
    13321 tcgtcaaatc tacaacatgt aacattcgaa tgtaaagaaa taggggattg caattttact
    13381 actgcaacag ttgataatgt catatttaaa tgtcgacgtt tacacaatgt gatttttatc
    13441 aaagcgagtg gcgaatatgt cgattttagc caaagtattc ttgatacagt tgacttctcg
    13501 cggagccagc ttactcatag taattttcgt gaatgtcaga ttagaaattc aaagttcaat
    13561 aattgttatc tttatgcttc gcacttcacc agagcagaat ttctttctga caaagaaata
    13621 tcatttatta aatcgaattt gacagctgtc gtgtttgatc atgtgcgaat atcgacaggg
    13681 aattttaaag acagcgttac acaacgaatg gtattatcta ttgattactc agatatattt
    13741 ggaaatgaat atctcgatgg ttatatcaat aacattataa aaatgattga tacattgcca
    13801 gatgatccag cgatattgaa atccgttctg gcagtaaaac tggtgatgca attaaaaatt
    13861 cttaatattg ttaataaaaa ctttattg
//
LOCUS       DAYPHN010000132        13813 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000132 DAYPHN010000000
VERSION     DAYPHN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.132"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(57..1595)
                     /gene="cadC"
                     /locus_tag="ACG0BW_003574"
     CDS             complement(57..1595)
                     /gene="cadC"
                     /locus_tag="ACG0BW_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFK7260498.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPDTDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(2212..2287)
                     /locus_tag="ACG0BW_003575"
     tRNA            complement(2212..2287)
                     /locus_tag="ACG0BW_003575"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2252..2254),aa:Phe,seq:gaa)
     gene            complement(2394..2969)
                     /locus_tag="ACG0BW_003576"
     CDS             complement(2394..2969)
                     /locus_tag="ACG0BW_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7260499.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(3006..4703)
                     /gene="dsbD"
                     /locus_tag="ACG0BW_003577"
     CDS             complement(3006..4703)
                     /gene="dsbD"
                     /locus_tag="ACG0BW_003577"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFK7260500.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINKASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVVNNSAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMLLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4679..5017)
                     /gene="cutA"
                     /locus_tag="ACG0BW_003578"
     CDS             complement(4679..5017)
                     /gene="cutA"
                     /locus_tag="ACG0BW_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFK7260501.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5132..6433)
                     /gene="dcuA"
                     /locus_tag="ACG0BW_003579"
     CDS             complement(5132..6433)
                     /gene="dcuA"
                     /locus_tag="ACG0BW_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFK7260502.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6551..7987)
                     /gene="aspA"
                     /locus_tag="ACG0BW_003580"
     CDS             complement(6551..7987)
                     /gene="aspA"
                     /locus_tag="ACG0BW_003580"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFK7260503.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8324..8800
                     /locus_tag="ACG0BW_003581"
     CDS             8324..8800
                     /locus_tag="ACG0BW_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFK7260504.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8816..10072)
                     /gene="yjeH"
                     /locus_tag="ACG0BW_003582"
     CDS             complement(8816..10072)
                     /gene="yjeH"
                     /locus_tag="ACG0BW_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFK7260505.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            10348..10641
                     /locus_tag="ACG0BW_003583"
     CDS             10348..10641
                     /locus_tag="ACG0BW_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFK7260506.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10685..12331
                     /gene="groL"
                     /locus_tag="ACG0BW_003584"
     CDS             10685..12331
                     /gene="groL"
                     /locus_tag="ACG0BW_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFK7260507.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12469..12822
                     /locus_tag="ACG0BW_003585"
     CDS             12469..12822
                     /locus_tag="ACG0BW_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFK7260508.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(12872..13741)
                     /locus_tag="ACG0BW_003586"
     CDS             complement(12872..13741)
                     /locus_tag="ACG0BW_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFK7260509.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         3594 a         3313 c         3473 g         3433 t
ORIGIN      
        1 aacatacaaa aactcaacaa caaatattgc cgagcataaa tcaacccggg agttacttat
       61 tctgaagcga gaaatttgtc gagataaggt acaacataag gaacagaagt ctggaatata
      121 ccattttcaa tccagtaaag ggtgtttgcc cctgggcgta aattaaaggc ggtgagatat
      181 gcatcagctg cttcccggtt catccccttc atttcataaa ccttgccaag caatacataa
      241 tttagccagg acatttcaag atcaatgcca gtatttatcg cctgataaga ctcatctgtt
      301 ttacctttta ccagagcact gaccgctttt atttgatata taatggacag gttattcaat
      361 tccggtagtg taacaatgtt atctatttct gtgttcagtg ctgctaattg tttttcatct
      421 aaaggatgtt gagaatggcg cacgatatca actaatgctt tttctgctct cgcgtaggta
      481 aattctgggg atgattgaac aatctcaccc aataattcac tggcacggtt caatgattta
      541 tcatcgccat gcagtaaata atcatgtgcc tgataaaaat tagttaataa cgcaccacga
      601 tgaggcaaaa tttgctggag catctcctgc attcgttgtg gccacggttg gtttaacgct
      661 tttgataaac tctccagtaa atcattttga atcgccagct gattaccgtt agtgatgaca
      721 taacgtttat ccagcatggt tgaaccatct gcattgtcta ccaattttac cgacataaag
      781 cattgttgag cacggtattg gcgctgatta acaaacgcaa tagataatgt tttaccggaa
      841 ctgctcggtt catcaatgtt gtagttgatt ttgtcatgca ccataaaggt ggagaaggtg
      901 ttaagtgatg tcgccaccaa atcacccacg cctatcgcgt aagagagctg atacggggaa
      961 ctccagctgt tacaactttt atttaccata ttaatgtcaa tatcgcgtgg attgagcaaa
     1021 atacgcgatt tgctcatcgg aagacgtgta tcaagacttg aaaacgctac cagtgctaca
     1081 cagataccta acgacaacag gaaaaaaaac catacccaaa aggtagtgaa tcgtttgctt
     1141 ttaacagggg attgttcagg tggtgttgcg gtgttttgaa tgttaagact gtgggaggga
     1201 gaatctgtgt caggaaccgc ctctggtata gggggaggcg aagatagcat tatttcctct
     1261 ccctcttctt cgctgtacca gataaccggc accattaatt tatagccgcg ctttggtaca
     1321 gtagcgatat agacaggact atcttcatca ttatctttta atgacttacg tagttctgag
     1381 atactctgcg tcacaacgtg attggtgaca atacttctct tccagacatt atcgataagt
     1441 tcatccctgc taagtacttc gccactgtgt tgagcaaaga aaaccagaag atcgattaat
     1501 ctcggctcaa gggtaagttg acgcccattg cggctaattt ggtttatgga cggagtaaca
     1561 agccattcgc caacgcgaac tacaggttgt tgcataatag aaactcattc gaaaagggaa
     1621 tgatgcaatg ataattgcca caacctattt ttaccatcta tagatgggtt tatttacata
     1681 ttattggtga atgcaagacg ttatttttac cagccataaa cttcttgatc acatagtatt
     1741 acgaaaggat tttactgaga accagaagta atattcctta ccatcaaaat tcatcatctt
     1801 tgccaaagaa aaatgttcag aaaataatcc atggaaaatt gcccggagcg cttactattt
     1861 ttatggattg ttagtctttg catgagcaag cggactgata catttctctt tgttctcatt
     1921 cagaaaatct catcagtcgg cgttctgccc gaggttgtgc tttatggatg cgttacgctc
     1981 ctgatgacgt catttgacgt tcaacagcat cacggggccg cgcgacattt cacgtcagtc
     2041 agtgctatcg ctcaggaaca aattttcccg aattgggata tgcccgcaaa ttgctggtga
     2101 tgtggggaga atctggttga gttcggtaga aatgattttt aggcagaacg cttaaattgt
     2161 ggcgtcctga aacgaaaaac ggacctccgt ggaggtccgt ttatatgaat ttggtgcccg
     2221 gactcggaat cgaaccaagg acacggggat tttcaatccc ctgctctacc gactgagcta
     2281 tccgggcaac ggggcgcatt aaaccgtaat ctgcacatct cgtcaaccta atttcagtaa
     2341 aagcgattca actgcttaag attgcggcaa atcgcttctt ttctgtgttt ctgtcaggtc
     2401 agtgcgccac cctggcggca gcgagcgaag cgcaggatat cttctgccag ccgatgtgcg
     2461 gtgtcgacat ccgcctggct acgattcacc agcattcggc ttaaacagcc ttccagcacc
     2521 agttccatct gctttgctac catcgccgga tcgtcaactt ccagcgtggt taacagttcg
     2581 tgggtgaaat cgtaggccgc gcttttttgc tgatcggcca gttgatgaat agggtggcca
     2641 ggatcgggat aaaacgtaca ggcagcgata aacagacagc ccggatagcg gttgttttta
     2701 acgcactccg ataacgcctg ataacgtgcc agcagctttt gttcggcggt ttgcgtttcg
     2761 tccagcatca gctgacgacg ccagacatct atctgttggc taagataacg cagcgcatcg
     2821 tagaggattg cctctttgtc tggccagaaa cggcgcagct cgtccagtgg ataatccaca
     2881 cgttcagcaa ccatctccag cgtggtgttg gcaatccctt gtaattctaa taatttcagg
     2941 gcttctccca gtacatcttc acgttgcacg ctattttcct ccgtctttcc cactgcaagt
     3001 gtcgttcacg gttggcgatc gcgcaaatgt gcgctgaagg tttcagcatc cataaagccc
     3061 gtgacgcgtg cttgtggatg ctcctggcct tgtccgtcaa aaaagagaat tgtcggtagg
     3121 ccaaggacat taagatgctt taacagcgcc acatcttgtg cgtcgttggc agtgacgttg
     3181 gcctgaagta ataccgtgtc tgctaacgct ttttgcacct gcgggtcgct gaaggtgtat
     3241 ttctcaaact ctttacaggc gacgcaccag tcggcataga gatctaacat caccggtttg
     3301 cctttggctt caacgagcgc ctgatttaac tcatctaccg ttttgatttg tgtaaagttg
     3361 agatgcgtct gagtttgcgc ggtatgcgtc gcaccaaatg cccaatcctg aagtgggcgc
     3421 acgctaacta atgccgctgc cagcaggatt atttgcacca cgcgcatcca gccgcgtttg
     3481 gcctgtaggc tggtgataaa ggcccagcca aagaatgcga caccaagcgc cgaccacaag
     3541 cgtaatcccc atatatcacc aatcactcgc tccagcagga agaccggcag tgcgaggatc
     3601 acaaaaccaa acgcggtttt gacttgttcc atccacgggc cgcttttcgg cagcaggcgg
     3661 ttaccaaaga cggttattag catcagcggc aggcccatgc ccaacgcata gagataaagc
     3721 gtgccgcccc ccagcaacat gttcccgctt tgggcgatat acagcagaat cgcgctaagc
     3781 ggtgcggtgg tgcatggtga acagatcagt ccggcaatcg cccccataac aaacacaccg
     3841 ccaggtgagc cgccctgttg gcgattgctc atcagcgtga gacgtgtttg cagcgaagag
     3901 gggagttgca gagtaaacaa gccaaacatt gacatcgcca gcaaggtaaa gacgatagcg
     3961 aggccaatga gcacgtatgg gtgctgtagc gccgcctgga actgtaaccc tgcggcggca
     4021 accaccagac ccagtgccgt gtaagtcagc gccatcccct gcacataaat aaaggtcagc
     4081 aacaatgctc tggcagtgga gagccgctgt ttaccgccca gcacgatacc agaaatcagt
     4141 gggtacattg gcagcacgca tggcgtaaag gcgataccaa taccgatcaa caacgcccag
     4201 agcgcggaaa agggcaattg cgcggtgggc tgctcttgct gcggaacaga cacaggctgt
     4261 ggcgctgagt tgttgacgac cacttcgctt aacggaacgg ttttggtttc tggcggataa
     4321 cagaaaccgg catcagcaca gccctggtag gtgacagtta acgtcgctcc cgcactcgcc
     4381 ttgttgatgg ttacgggaag cgtcagccga tcgcggtaaa tctcgctttt gccgtaaaac
     4441 tcatcttcat gccagacgcc ttgcggcagc tgtacgtcgg cgattttcgc gtgttccggc
     4501 gtaatgcgga tctgtttacg gtagaggtaa taaccgtctt tgatctgcca ggtcagattc
     4561 aggtcatgtt ggttttgctg aaaatcaaaa gcaaaggctt gatccgcggg gacaaattgt
     4621 gaacgtcccg gcgcgtcgaa taatccggca aaaacggaag tgctgcaaag tagcaggatc
     4681 agcgtaaaga tgcgttgagc catgagaggt aatctgtgtc tccgtgtgta acaggtaaaa
     4741 ccagaagttc cggggtttga tatggatgat gagacttcag gcattccagc agtgcctgct
     4801 ggtgagatac ggtagttttt aaaatcatct gcacttcgta ttcttgctcc agcttacctt
     4861 cccagtaata aagagaggtt gcgccgggga tcaaggtcgc gcaggccgcc agtttttccg
     4921 ccagcacttt ggcggctaaa tcctgggctg tcgcttcatc cggtgccgta catagcacca
     4981 cgacagacgc ggtattcgaa cttttttcat caagcataaa cacctcgcaa gaacagatgg
     5041 aaccgcaaga gagaggtcac tatacaacgg acgggggaag gatgttagtc ggcgggaaag
     5101 aaagcggggc ctgaagcccc gcgattgatc attacagcat gaagctaccc agcacgaagc
     5161 cgaagcaaac ggccagggca acacccagag tacccgggat gaagaacgga tggttgaaga
     5221 cgaatttacc gatacgggtt gtacccgtgt catccatctg taccgcagca accagcgtcg
     5281 ggtaggtcgg cagaatgaac agacctgaca ccgcagcgaa agaagcaaca gcggtcagcg
     5341 gagaaacgtt cagtgccaga gccatcggca tcagtgcttt tgcggttgca gcctgagagt
     5401 acagcagagc agaagcaaag aagaagatga cggccagcag ccacggatga ccctgaatca
     5461 cttcaccagc ggtatctttg atccagtcga tgttgttgga aacgaaagta tcgcccagcc
     5521 acgcaacacc caggatacaa atacaggcgc tcatacctgc tttgaaggtg ctggagttga
     5581 ggatgttgtc ggtatcgact ttacagataa cggtggtcag agttgcaacg ctgagcatga
     5641 tgatcaggat tgcgttggtg gtgttcatca gcggtttttc aaccagaccc atgcttgggc
     5701 tgttgatgat tgcatagata accacgccaa ctacgcccag caggaatagc cagacggacg
     5761 tttttgcacc ggatttgatt tcaatctgct tttcaccgcg cagttcaacc aggccttctt
     5821 ccagacgctt gcgataaatc ggatcgtcag agagtttgga gttgaacagc atagtgacca
     5881 ggaaggacat caccagaacc gccagcaggg tggacgggat gaccacggag agcagatgga
     5941 ggtagctgat gccatgacct tccattacgg aagacatgta aaccactgct gctgagattg
     6001 gcgatgcggt gatcgcaatc tgcgcggata ccactgcagt agacagcgga cggcaaggtt
     6061 tgacgccttg ttccttcgca acttcagcga taactggcag tgtcgccaga gagatgttgc
     6121 cagtaccagc aaagattgtc aggaaatagg tcacgatcgg cgcgaggatc gtgatgtatt
     6181 tcgggttacg gcgcagcagc ttttctgtct gatgaaccag atagtccaga ccgccagcaa
     6241 cctgcatggc agaaatagcg gcgataaccg ccatgataat ggaaatgaca tcgaacggga
     6301 tgttaccggg tttaacgcca atagcggcaa gaaccagcac ccccaatccg cctgcaaaac
     6361 caatacctat tccccccaat ctggcgccca agaagatcgc cagcaaaact atgatgagtt
     6421 ctacaactag catattagcc ttccttgttt tttaacaagt tgatattgga ttgttatttt
     6481 taagttactg ctcacaagaa aaaaggcacg tcatctgacg tgccttcttt atttgtacta
     6541 ccctgtacga ttactgttcg ctttcatcag tatagcgttt tgctttgtaa gccgggtgca
     6601 tcagattctg tacggagaaa atatcgtcaa gttccgcttc agtcaacaga ccgcgttcca
     6661 gaacgacttc acgtacactc ttaccggttt cggcacagat tttacccacg atgtcaccgt
     6721 tgtggtgacc gatgaacggg ttcaggtaag taacgatacc gatagagttg taaacgtaac
     6781 cttcgcacac ttctttgtta gcagtgatgc cgttaatgca tttttccagc aggttgtagc
     6841 aagcgttggt cagaatgtga acggattcaa acatggcctg gccaatgacc ggctccataa
     6901 cgttcaactg cagctgacct gcttctgcgg ccatggtaac agtggtgtcg ttaccgatga
     6961 ctttgaagca tacctggtta accacttccg gaacaaccgg gtttacttta gctggcatga
     7021 tggaagagcc cgcctgcagt tccggcaggt tgatctcgtt caggccggca cgtgggccag
     7081 aagagagcaa gcgcaggtcg ttacagattt tggacatctt cacagccagg cgtttcagcg
     7141 cgccgtgaac cataacataa gcgccgcagt cagaggtcgc ttcgatcagg tcttcagccg
     7201 gtacgcatgg gaagccagtg acttcagcca gttttttcac tgccagcgga gagtactctt
     7261 tcggcgtgtt cagaccagta ccgattgctg ttgcgccaag gttaacttcc agcagcagtt
     7321 cagcggtacg ttggatattt ttcacttctt ctttcagcag gatgctgaaa gcgcggaatt
     7381 cctgaccgag ggtcatcggt actgcgtcct gcagctgggt acgacccatt ttcaggatgt
     7441 cctggaattc gacagcttta cgttcaaagc cttcacgcag ttggttaatc gcatctacca
     7501 gcttaatcag ggaagagtaa actgcgatac ggaaaccggt cgggtaggcg tcgttagtgg
     7561 actgacattt gttaacatgg tcgttcgggt tcaggtactg atattcacct ttctggtgac
     7621 ccatcagttc cagaccgata ttggccagca cttcgttggt gttcatgttt acggaagtac
     7681 ctgcgccgcc ctggtagacg tctaccggga actgatccat gcattttccg ttgttcagga
     7741 cttcatcaca tgcggcaatg atggcattcg ctacactttt aggaatggtt tgcagctctt
     7801 tgtttgccat agctgcggct tttttaacca ttaccatacc gcgaacaaat tcaggaatat
     7861 cactgatttt gttgttgctg atatagaagt tttcaatcgc tctcagagtg tgaacaccat
     7921 agtaggcatc agctggaact tccctggtac ccaacagatc ttcttcgata cgaatgttgt
     7981 ttgacatgtg aaccttcttt ttcaagctgc caatgatttg ctttaaacac acagaatata
     8041 tgtggtttcg aatgtttttc gaccgacgat tatcccctgc atcgaccgaa tacccgagat
     8101 catatgctgc ttgaggattt ctaccgtaat ctggatcact ttaagtgtcg gtttttaccc
     8161 cttaattatt aatttgtgaa atagatcacc gctttgggat tactaccaaa aatagttgcg
     8221 caaacatctt gaaattttgc taatgaccac aatataagct gaacgcgatt cgcaacccat
     8281 tcaggtagcc gggtttaacc ggctgctatt acaggagaaa cctttgcgct ggttaccttt
     8341 tattgccatt tttctttatg tctatattga gatttcaatc tttattcagg ttgcccatgt
     8401 attgggggta ttgctgaccc tcgtgctggt tatattcacg tcagttatcg gtatgtcact
     8461 ggtacgcaac cagggcttta agaatttcgt gctgatgcag caaaaaatgg cggcgggtga
     8521 aaacccagcg gcggagatga ttaaaagtgt ttcgctgatc attgctggtt tgctgctttt
     8581 attaccgggc ttttttaccg acttcctcgg tcttctgctt ttattgccgc cggtgcaaaa
     8641 gcatctgaca gtgaagttga tgccgcattt gcgcttttct cgcatgccag gcggcggttt
     8701 tagcgccggg accggtggcg gtaatacttt tgatggtgag taccagcgaa aggatgatga
     8761 ccgcgaccgc cttgatcata aagacgatcg ccaggattaa tgtcgaaacg ccggattatg
     8821 tggttatgcc attttccggc gtttttcgtt ttggcagaaa cagccataac cccgccagca
     8881 tgatcagcgc atagagactt ttccagccga ccattgccag taacagaacg cataacagcc
     8941 cgccaaccac cgccagtagt cgataacgtc cttgcaataa tttacaacct gccagcatgc
     9001 ataacagata aatcataata aagatgccat tggcataaat aataagagcg tccagattga
     9061 tctctaaagc atgaatcacc aacgtgctca ccacgcagca gccgagtacc gcattgaggg
     9121 cattattcgg gatatgacga gaagagaggc gtgccaggta gtggtcagga ttatgttgcg
     9181 cctgcgacca gaccaggcgg gcgaagctct gtatataaat gttgagactg gcaaagcagg
     9241 ccagatagcc aatcacgcag gcaatccata acgctcctac accgaacagt tgcactacaa
     9301 tttttggaag cgatgctgct gccgccattt gttcaccata ggcgtcgaag tgtaagacga
     9361 ctaccgtaca gccccagtag actaatcctg ccagcagcag accaatcatc aaagcacgag
     9421 gaaaatcacg ctctggattt ttaaattccg aggcaagatg ggcaaatgct tccagaccga
     9481 caaagcacca gaacatcact gataacgcag caaataaccc ggtaagttcg atattaccag
     9541 gtgcagggaa ggggatactc gcaggtttga tatcgcccgc ccaccagata gcgacaatca
     9601 gcgcgacgat aagtccggca ataactgttt gtagattagc actggaactg gcaccgcgag
     9661 taccgatata ccacaccagc gccagcgtac cgagttctgc caacaacagt tgccagctat
     9721 gccagccaaa cattgcctgg ccgaacccgg cagcaatttg taacgcggca ggcaaaccca
     9781 cgggaatgac cgataaaaac aaccagccgg tgactcgctc aagccgcgaa ccaaacgcca
     9841 taccgacgaa gtgcgcgaca ccgcctgcgc tgggatagtg gcgacccaga atcgcaaaca
     9901 caatcgcaat cgggaacact aagataatca aaacgggcca cgcccacagg ctgttattgc
     9961 ctgctaccag tgcagctaac gcaggaacgg caaacacgcc ggtgcctaat aatgacgtcg
    10021 atagcaggcc aatgccctgg gccagcccca gttcttgttt gagtccactc atgggttgat
    10081 gtccgattgc gcccaaatta tgggcaactg cgtagatttt cgatggtagc acaatcagat
    10141 tcgcttatga cggtgatgaa gaaattgcga tgaaatgtga ggtgaatcag ggttttcacc
    10201 cgattttgtg ctgatcagaa ttttttttct ttttccccct tgaaggggcg aagcctcatc
    10261 cccatttctc tggtcaccag ccgggaaacc acgtaagctc cggcgtcacc cataacagat
    10321 acggactttc tcaaaggaga gttatcaatg aatattcgtc cattgcatga tcgcgtgatc
    10381 gtcaagcgta aagaagttga aactaaatct gctggcggca tcgttctgac cggctctgca
    10441 gcggctaaat ccacccgcgg cgaagtgctg gctgtcggca atggccgtat ccttgaaaat
    10501 ggcgaagtga agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac
    10561 ggtgtgaaat ctgagaagat cgacaatgaa gaagtgttga tcatgtccga aagcgacatt
    10621 ctggcaattg ttgaagcgta atccgcgcac gacactgaac atacgaattt aaggaataaa
    10681 gataatggca gctaaagacg taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg
    10741 cgtaaacgta ctggcagatg cagtgaaagt taccctcggt ccgaaaggcc gtaacgtagt
    10801 tctggataaa tctttcggtg caccgaccat caccaaagat ggtgtttccg ttgctcgtga
    10861 aatcgaactg gaagacaagt tcgaaaatat gggtgcgcag atggtgaaag aagttgcctc
    10921 taaagcgaac gacgctgcag gcgacggtac caccactgca accgtactgg ctcaggctat
    10981 catcactgaa ggtctgaaag ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg
    11041 tatcgacaaa gctgttaccg ctgcagttga agaactgaaa gcgctgtccg taccgtgctc
    11101 tgactctaaa gcgattgctc aggttggtac catctccgct aactccgacg aaaccgtagg
    11161 taaactgatc gctgaagcga tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga
    11221 cggtaccggt ctgcaggacg aactggacgt ggttgaaggt atgcagttcg accgtggcta
    11281 cctgtctcct tacttcatca acaagccgga aactggcgca gtagaactgg aaagcccgtt
    11341 catcctgctg gctgacaaga aaatctccaa catccgcgaa atgctgccgg ttctggaagc
    11401 tgttgccaaa gcaggcaaac cgctgctgat catcgctgaa gatgtagaag gcgaagcgct
    11461 ggcaactctg gttgttaaca ccatgcgtgg catcgtgaaa gttgctgcgg ttaaagctcc
    11521 gggcttcggc gatcgtcgta aagctatgct gcaggatatc gcaaccctga ctggcggtac
    11581 cgtaatctct gaagagatcg gtatggagct ggaaaaagca accctggaag acctgggtca
    11641 ggctaaacgt gttgtgatca acaaagacac taccaccatc atcgatggcg tgggtgaaga
    11701 agctgcaatc cagggccgtg ttgctcagat ccgtcagcag attgaagaag caacttctga
    11761 ctacgaccgt gaaaaactgc aggagcgtgt agcgaaactg gcaggcggcg ttgcagttat
    11821 caaagtaggt gctgctaccg aagttgaaat gaaagagaaa aaagcacgcg ttgaagacgc
    11881 cctgcacgcg acccgtgctg cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct
    11941 gatccgcgta gcgtctaaac tggctgacct gcgtggtcag aacgaagacc agaacgtggg
    12001 tatcaaagtt gcactgcgtg caatggaagc tccgctgcgt cagatcgtcc tgaactgcgg
    12061 cgaagaaccg tctgttgttg ctaacaccgt taaaggcggc gacggtaact acggttacaa
    12121 cgcagcaacc gaagaatacg gcaacatgat cgacatgggt atcctggatc caaccaaagt
    12181 aacccgttct gctctgcagt acgcggcttc tgtggctggc ctgatgatca ccaccgagtg
    12241 catggttacc gacctgccga aaaacgatgc agctgactta ggcgctgctg gcggtatggg
    12301 cggcatgggt ggcatgggcg gcatgatgta attgccctgc acctcgcaga aataaacaaa
    12361 cccccgggca gaaatgtctg ggggtttttc ttttggtcat ctttctagta taagattcag
    12421 acacggacga cgcgagtggc gtccagctca ttgattatgg ggaataacat gcacgtaaaa
    12481 tacttagcag ggattgtcgg tgccgcgcta ctgatggcgg gttgtagctc cagcaacgaa
    12541 ttgagtgctg ccggtcagag tgtacgcatt gtggacgagc agccaggcgc agagtgccag
    12601 ctgattggta ctgcgacagg taagcaaagc aactggcttt ccgggcaaca cggtgaagag
    12661 ggcggttcta tgcgcggcgc agcaaacgat ctgcgcaacc aggctgctgc aatgggcggt
    12721 aacgtgattt atggcatcag cagcccgtcg caggggatgt tgtccagttt tgtcccgacg
    12781 gatagccaga ttatcggcca ggtatataag tgcccgaact gatgggatcc agaggctgga
    12841 tacttccagc ctcgtagtaa aacagatggg attatttcgc ctgtggtgca gttttgctgg
    12901 tggattgaac acgtaagtga tgtagcgcgc gtaagcattg atcctgggtt agcggttggg
    12961 cgttatttgc cgctacggtg cgaacataga cctgacatgg tacacccgcg agttttttca
    13021 gtcgtgggct gaaatcaagc attcggcgag tgatcgcctc cacttcttta cccggcgttg
    13081 cttcacgcgt tttcaccact gcgccgctaa attgttcttt gccactttca tctttaaagc
    13141 ggtaaagcac cgccagataa tggttgaaca agttatgttt ccattccggt tgggaatagg
    13201 tgtcatattc cagattgcca ttgtcctggc agtcaacatc gtataacggt aggaaatcta
    13261 acagtgaggt aatacgtaat gccagttcac gcatttccgc attattaatt gcatgaatga
    13321 ttgctcgcgc cagcatttca atctgcaatt cattgatgac taattcacat ttgctaccgt
    13381 catgtagcgt taatacgaag gtaagatttt caccctgatt atcggtaagt tccagcgtgt
    13441 taacccgacg attaatatcg gcatttttaa gctcatcaac cagaatttct gggatatttt
    13501 ctgccatttt cttaatgact ttatcgcgag cttgctcata ctgaaggcgt gcagcagcat
    13561 ccagtttatg cttttgatgc aaacggcttt ccagcgcaat cagaaggtcg cgcagttcca
    13621 tgacagacag aaagaacaac gattctttat tgcgaggctc tttaattttc agcgccagtg
    13681 caatgaaatt attgctcttg cgaataacac cggtattaac acccttgatg cttatggcca
    13741 tgtaggttct ccctaaccat ttctcaatta aataattaat tttaatttat aagccagata
    13801 aatgggcttg gta
//
LOCUS       DAYPHN010000133        13715 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000133 DAYPHN010000000
VERSION     DAYPHN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.133"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..101)
                     /locus_tag="ACG0BW_003587"
     CDS             complement(<1..101)
                     /locus_tag="ACG0BW_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7260510.1"
                     /translation="MILWSVRKETDIRRGRHCVFLMHVHQVFVTKYRR"
     gene            109..1305
                     /gene="insQ"
                     /locus_tag="ACG0BW_003588"
     CDS             109..1305
                     /gene="insQ"
                     /locus_tag="ACG0BW_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFK7260511.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTSVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPPEMI"
     gene            1361..1468
                     /locus_tag="ACG0BW_003589"
                     /pseudo
     CDS             1361..1468
                     /locus_tag="ACG0BW_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1452..2090)
                     /gene="ytfB"
                     /locus_tag="ACG0BW_003590"
     CDS             complement(1452..2090)
                     /gene="ytfB"
                     /locus_tag="ACG0BW_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFK7260512.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            2308..2928
                     /gene="fklB"
                     /locus_tag="ACG0BW_003591"
     CDS             2308..2928
                     /gene="fklB"
                     /locus_tag="ACG0BW_003591"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFK7260513.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            3237..4640
                     /gene="cycA"
                     /locus_tag="ACG0BW_003592"
                     /gene_synonym="dagA"
     CDS             3237..4640
                     /gene="cycA"
                     /locus_tag="ACG0BW_003592"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFK7260514.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHDKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            4774..5937
                     /locus_tag="ACG0BW_003593"
     CDS             4774..5937
                     /locus_tag="ACG0BW_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HFK7260515.1"
                     /translation="MVKRSWLLIAALPLSISPSWGADFYYRQHEKGTVYVAEQKGKED
                     EILSELPDVNFSRLWRIANLANKQETRLLSDFNPDKFDCDDEGDCQHTWLTDGRSVLW
                     AGKVLKNPSGEPNVDAASFKAFGAFAADKRSIYFDGQRTDDNSGDKQVDMSTLEETDI
                     WNLLRDKNSLWHKGHWLGSADGFQILRHDSSLQFVVMTNSQVIVNGKPLPADRKTFQI
                     IRWMPGERLVYRDKSGEHDYTLEDIGHSCALFNIGLKNVSRLKQEATPAGSDCVYETL
                     KGVDPEYFTLLTGSVGYYKDQFYKVKTNALGEGELITPKPEDVFELLDRESMVTGYMY
                     ITTDGQLYSHELSGLTTIDGKHYEQTEWLRYNPISAEWSTVKQPPSGLKPLFK"
     gene            5971..7455
                     /locus_tag="ACG0BW_003594"
     CDS             5971..7455
                     /locus_tag="ACG0BW_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260516.1"
                     /translation="MLPIKPVCLALALFSTASIACSGVPYAAFYYQPWGQGTKYIVED
                     SDGYGIDFPDIHLPQVKFKQLRRVPNGTDGEYDSPDDINNDFVRVAPFSGYRSNGGWR
                     FDWLTDGRHILWAGKIVQNPPGTPKVDVATFRAWGRFAADKDSLYFDGERTDDNRDEK
                     QVDMDSLQQVGGSGDSADVLKDRHNIYFQGHWLGSAQGYSILGVKSWDRRIILFSPNS
                     CQSKSNPGPWDTIWRTKSQVFVNGDAINADPDTFHIVRWIAGSLLIYRDKDGEKRYAF
                     GKNCRSTFDLQRDKVTWLTRDASRRANDCRVATIPDVDPEYFRPLTENVAQYKDSLYL
                     VKYVSAVEKTLSVIHLPDPQQELQEGVNIVGDKVYFIASDDVTIFDINGQWQWYKSPD
                     GTPLNYLAHDDRYTYFIDEDTVEHFELKGQWTWFKYANGQLSDTFAHDDHYIYYVGDG
                     LVRDAKRRNETRQLDAAHLDKNGSLLTVEGEYTSYNNELFPLND"
     gene            complement(7500..8162)
                     /gene="ytfE"
                     /locus_tag="ACG0BW_003595"
     CDS             complement(7500..8162)
                     /gene="ytfE"
                     /locus_tag="ACG0BW_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFK7260517.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(8270..9235)
                     /gene="ytfF"
                     /locus_tag="ACG0BW_003596"
     CDS             complement(8270..9235)
                     /gene="ytfF"
                     /locus_tag="ACG0BW_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFK7260518.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLLFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLIEPVSES"
     gene            complement(9344..10204)
                     /gene="qorB"
                     /locus_tag="ACG0BW_003597"
     CDS             complement(9344..10204)
                     /gene="qorB"
                     /locus_tag="ACG0BW_003597"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFK7260519.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            10292..10672
                     /gene="ytfH"
                     /locus_tag="ACG0BW_003598"
     CDS             10292..10672
                     /gene="ytfH"
                     /locus_tag="ACG0BW_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFK7260520.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(10801..12744)
                     /gene="cpdB"
                     /locus_tag="ACG0BW_003599"
     CDS             complement(10801..12744)
                     /gene="cpdB"
                     /locus_tag="ACG0BW_003599"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFK7260521.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKGT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLIFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIAEESKRAGEIHPAADNNWRLAPITGDKKLDIRFETSPSDKAAAFIKDKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            12934..13674
                     /gene="cysQ"
                     /locus_tag="ACG0BW_003600"
     CDS             12934..13674
                     /gene="cysQ"
                     /locus_tag="ACG0BW_003600"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFK7260522.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         3312 a         3383 c         3562 g         3458 t
ORIGIN      
        1 cgtcggtatt tggtgacaaa gacctggtga acatgcatca ggaaaacaca atgccttcca
       61 cgccggatat cggtttcttt tctcacagac caaagtatca tttggcctgt gaaacgacta
      121 caggcattta aattccagtt aagacccggt ggtcaacagg agcgtgaaat gaggcgcttc
      181 gcaggcgcat gtcgtttcgt tttcaatcgt gctctggcac gtcagaatga gaatcatgag
      241 gccggtaata aatacatccc ttacgggaaa atggcttcct ggctggttga gtggaaaaac
      301 gccactgaaa cgcaatggct taaagattcg ccctcacagc cattgcaaca gtcactgaaa
      361 gaccttgagc gggcttacaa aaacttcttc cagaagcggg ctgcttttcc ccgattcaaa
      421 aagcggggac agaatgatgc attccgctac ccgcagggcg ttaagctcga tcaggaaaac
      481 agccgtattt ttctgccgaa acttggctgg atgcgctacc ggaatagccg tcaggtcacg
      541 agtgttgtga aaaatgtcac tgtcagccag tcctgcggta agtggtacat cagtattcag
      601 actgaaaatg aagtatcaac tccggttcac ccttcagcat caatggtcgg gctggatgct
      661 ggcgtggcta aactcgccac gctgtcagat ggcacagtct ttgagcctgt aaacagtttt
      721 cagaaaaacc agaagaagct ggcgagactt cagcgacagt taagccgcaa ggtcaaattc
      781 agcaataact ggcagaagca gaaacgcaaa atacagcgac tgcattcctg tatcgcaaat
      841 atccgcaggg actaccttca caaagtcaca acgaccgtca gcaaaaacca cgcaatgata
      901 gtcattgagg atttgaaggt cagcaacatg tcaaagtcag cagcgggtac ggtcagccag
      961 ccggggcgca atgtccgggc aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg
     1021 tatgaaatgc gccgccagct tgagtacaag cagctctggc gtggcggtca ggtgcttgct
     1081 gttccgccag cgtacacaag ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaat
     1141 cgcctgtcac aaagtcaatt cagatgccag gcatgtggat atacagcgaa cgccgatgta
     1201 aatggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
     1261 cagtcaggcc gctcgttgaa gcaggaaccc cccgaaatga tttaggcgac agcctgaacg
     1321 tagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcacc
     1381 tcaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
     1441 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
     1501 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
     1561 attttcacca tctgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
     1621 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
     1681 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
     1741 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
     1801 gcgaccgggg ccacctgatc aggatcattt tgtggcgtaa ctagctgcgc ccgcagctgt
     1861 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccact
     1921 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
     1981 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
     2041 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
     2101 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     2161 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     2221 ttgtcatcat tcccgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     2281 actttatacc ctaagaaagg aaatacgatg accaccccaa cttttgacac catcgaagcg
     2341 caagcaagct acggcattgg tttgcaggta gggcaacaac tgagtgaatc tggcctggaa
     2401 gggctgctgc cagaagcact ggttgcaggc attgctgatg cgctggaagg caaacatccg
     2461 gctgttccgg ttgatgtggt gcaccgcgcg ctgcgtgaaa tccacgaacg cgccgatgcc
     2521 gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg tgaaatacct ggaagaaaac
     2581 gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc aattccgcgt gatcaaccag
     2641 ggcgaaggcg caattccggc acgtaccgac cgcgttcgtg ttcattacac cggaaaactg
     2701 atcgacggca ccgtgtttga cagctccgtt gctcgcggcg agccagctga attcccggtt
     2761 aacggcgtga tcccaggctg gattgaagcg ctgactctga tgccggtagg ttctaaatgg
     2821 gaactgacta tcccgcagga actggcatat ggtgagcgtg gcgcaggcgc gtccatccct
     2881 ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa tcctctaagc agcgcattct
     2941 gttcccctcg agccagaggg gagcaggcat tcagcaataa acccttcagt ttgccaaacg
     3001 gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt ttgtgagctg tttcgcgtta
     3061 tcaccgtgat atgacactca ctttaaacat aaaattaaca ttagatctaa atcttagtat
     3121 tcatcccgcg tattgttacc taatatcgat gagtcccgat acagattcgt cgtatcatag
     3181 actgactaaa ggccgtagag cctgaacaac acagacaggt acaggaagaa aaaaacatgg
     3241 tagatcaggt aaaagtcgtt gccgatgatc aggctccggc tgaacagtcg ctacggcgca
     3301 atctcacaaa ccgacatatt cagcttattg ccattggcgg tgccattggt acggggttgt
     3361 ttatggggtc cggcaaaacg attagccttg ccgggccgtc gatcattttt gtttatatga
     3421 tcattggttt tatgctcttt ttcgtgatgc gggcaatggg ggaattgctg ctttcgaatc
     3481 tggaatacaa atcttttagt gacttcgctt ccgatttact cgggccgtgg gcaggatatt
     3541 tcaccggctg gacttactgg ttctgctggg ttgtaaccgg tatggcagac gtggtggcga
     3601 tcacggctta tgctcagttc tggttccccg atctctccga ctgggtcgcc tcgctggcgg
     3661 tgatagtgct gctgctgacg ctcaacctcg ccaccgtgaa aatgttcggt gagatggagt
     3721 tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct gatagtcgtc ggcctggtca
     3781 tggtggcgat gcactttcag tcaccgaccg gtgtagaagc gtcattcgcg catttgtgga
     3841 atgacggcgg ctggttcccg aaaggtttaa gtggcttctt tgccggattc cagatagcgg
     3901 ttttcgcttt cgtggggatt gagctggtag gtacaacagc tgcggaaacc aaagatccag
     3961 agaaatcact gccacgcgcg attaactcca ttccgatccg tatcattatg ttctacgtct
     4021 tcgcgttgat tgtgattatg tccgtgacgc cgtggagttc ggtagtcccg gagaaaagcc
     4081 cgttcgttga gctgtttgtg ttggtaggtt tgcctgcggc tgccagcgtg atcaactttg
     4141 tggtgctgac ctctgcggcg tcttccgcta acagcggtgt cttctctacc agccgtatgc
     4201 tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt cgctaaactc tctaagcgcg
     4261 cagtacccgc gaaagggctg accttctctt gtatctgtct gctcggcggc gtggtgatgt
     4321 tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat tacaaccgtt tccgcgattc
     4381 tgtttatgtt cgtctggacg attatccttt gctcgtacct ggtgtaccgc aaacagcgtc
     4441 cacacctgca tgataagtcg atctacaaga tgccgctcgg caagctgatg tgctgggtat
     4501 gtatggcatt ctttgtgttc gtggtcgtgc tgctgacact ggaagatgat acccgccagg
     4561 cgctgctggt taccccgctg tggtttatcg ctctggggct gggctggttg tttattggta
     4621 aaaaacgcat ggcgaagtaa tgctgcgttt atagctctga aaggcacctg cgggtgcctt
     4681 tgtctttata tattcttaaa aatatctacc atcatagaca gccctcaggc gcgagcttac
     4741 cgtgaatctt tgccactcgt tgcagagttc actatggtta agcgcagttg gttattaatc
     4801 gccgcattac ctctctccat ttcaccttcc tggggcgcgg atttttatta ccgccagcat
     4861 gaaaaaggca cggtttacgt tgccgaacag aagggaaaag aagatgagat cctctccgaa
     4921 ttaccagacg ttaatttttc ccgcctctgg cgcattgcca atttagccaa taaacaagaa
     4981 acccggctgc tctccgattt taatcccgat aagttcgact gtgatgacga gggggattgc
     5041 cagcatacct ggctcaccga tggacgttct gtcctttggg caggcaaagt cctgaaaaat
     5101 ccatccggtg aacctaatgt cgatgccgcc agttttaagg cattcggcgc tttcgctgct
     5161 gataaacgca gtatctattt tgacggccag cgtaccgatg ataatagcgg tgataagcag
     5221 gtggatatgt caacgcttga agagacggac atctggaatt tactgcgtga taaaaatagt
     5281 ctttggcata aggggcactg gttgggcagc gctgacgggt ttcaaatcct gcggcatgat
     5341 tcatccctgc aatttgttgt aatgacaaat tcgcaggtga ttgttaatgg caagccgctg
     5401 cccgccgatc gcaaaacctt tcagataatc cgctggatgc ctggcgaacg gttagtttat
     5461 cgcgataaaa gcggcgagca tgactatacg ttggaagata ttggtcacag ttgtgcgtta
     5521 tttaatatcg gtttgaagaa tgtgtccagg ctcaaacaag aagcgacgcc tgcgggcagt
     5581 gattgtgttt atgaaacgct taaaggagtt gatccggaat attttacctt acttaccggg
     5641 tcggtaggct attacaaaga tcaattctat aaggttaaaa ccaatgcatt aggtgaaggt
     5701 gaactgataa cacccaagcc agaggatgtt tttgaattat tagataggga aagcatggta
     5761 actggctaca tgtatatcac tacagacggg caactctaca gtcacgaact tagtggatta
     5821 accacaatcg acggaaaaca ctacgagcaa actgaatggc tgcgttacaa ccccatttca
     5881 gcagaatggt ctacggtgaa gcaacccccg agcggtctga aacccttatt taaataacct
     5941 cttacacgct aacagcttca ggatgatgct atgttgccaa ttaaacccgt ttgtctcgca
     6001 ctggctttat tttcaaccgc ttcgatagcc tgctccggcg tgccttatgc tgcgttttac
     6061 taccagccgt gggggcaagg cacaaaatat atcgttgaag atagtgacgg ttacggtatc
     6121 gattttccgg atattcattt gccgcaggta aagttcaaac aacttcgccg cgtacccaac
     6181 ggcactgatg gtgaatacga ttcacctgat gatatcaata atgattttgt gcgggtggct
     6241 cccttctctg gctatcggtc aaatggcggc tggcgtttcg actggttaac cgatggccgc
     6301 catattttat gggcagggaa aatagtccag aacccgccgg gaacaccgaa agttgatgtt
     6361 gcaacattcc gcgcgtgggg gcgttttgcc gccgataaag atagtctcta ttttgatggt
     6421 gaacgcacgg atgataatcg cgacgaaaag caggtcgata tggattcgtt gcaacaagtc
     6481 ggcggttcag gtgattccgc ggatgtgctg aaagatcgtc acaatattta ctttcagggg
     6541 cactggttgg gcagtgcgca agggtattct attcttggcg ttaaatcctg ggaccgacga
     6601 attattttat tttcccccaa tagctgccaa agcaaaagta atccgggacc ctgggataca
     6661 atctggcgga ctaaatcaca ggtctttgtg aatggagatg ccatcaacgc cgatccggat
     6721 acctttcata ttgtgcgctg gatagccggt tcactgctaa tttatcgcga taaagacggg
     6781 gagaaacgtt atgcgttcgg taaaaattgt cggagtacgt ttgacctgca gcgggataaa
     6841 gtaacatggt tgacgcgtga tgcttccagg cgtgctaatg actgccgggt tgcgactatt
     6901 cctgatgttg atccggaata ttttcgcccg ctgacggaga atgtggcgca atataaagat
     6961 tcgctctatc tggtgaagta tgtttctgcg gtcgaaaaaa cgctgtcggt cattcattta
     7021 ccagacccgc aacaagaatt acaggaaggt gttaatattg taggggataa agtttatttc
     7081 attgcgagcg atgatgtgac tatttttgat attaatgggc aatggcagtg gtataaaagc
     7141 cccgatggca caccattaaa ttatttagca catgacgatc gctatacgta ttttattgat
     7201 gaagacacgg ttgagcattt tgaactgaaa ggccaatgga cgtggtttaa atacgccaat
     7261 ggtcaattat cagatacctt tgctcatgat gaccactata tttattacgt aggcgatgga
     7321 ctggtacgtg atgcgaagcg gcgtaatgag actcgacaac tcgatgcggc gcatcttgat
     7381 aaaaacggca gtttgcttac cgttgaaggt gagtacacca gttacaacaa tgaattattt
     7441 ccactgaatg actgaataat caacgatcct gcccatgtca ggatcgttga tcgaacaaat
     7501 cactcacccg ccagcgcgcg tgggaacagc acattatttt ccaggctgat gtgctccatc
     7561 aggtcatcaa tcagttcatt aatgccgtta tacatcgctt tccaggtggt gcaggcttct
     7621 ggcggcggtg tgacgttatt ggtagtgtgt ttgattactt ccagcagttc acccgcttca
     7681 tcgtgctcgc tttccattac gctgattggc cccattgcct ggctgcccat gccttgtttg
     7741 atcatcggga agaggatctg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc
     7801 atagtcaggt attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgttcg
     7861 actttagtcg cttgcaggat cagctccggc agttgctcgc ggtgacgatc gtggtagcgc
     7921 acgatgatat ggtcgatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt
     7981 tgttcagcaa gctttgccag ctcagcttca atgacctcaa catccagttc tttacgtgcc
     8041 gccgcacgcg ccagcgtttg cttaccgccg cagcagtaat ccatatcata tttacgaaac
     8101 agagccgaag cgcgagggat agagagcgcc agttcaccta aaggttggtc gcgataagcc
     8161 atagctgata cctcattctt aataagataa gatgtatttt aaatgcatct ttaaggtgaa
     8221 tgctataacc cttacgtagc agaggcttat ttaactcact gccagcgtgt caactttcag
     8281 agacaggttc aattaagggt ttttccggtt ttgctctgac cgcaatgacc acgccaacca
     8341 ctaacagcgc gataccgctt agcgtcatta gcggcggcat ttgctggcgg agtaaaaagg
     8401 tgtacagcaa acctgccagc gtttcgaaaa caatcagcgg cccgagaatc acggtcggta
     8461 atcgctggct ggcgacgttc cagcagagtg caccgaccca tgagcaaagc acggctatcg
     8521 caatcatcag actaataaat aaaagcggac gggggccaaa gggtagggag aagtccggcg
     8581 tttgcgtatt cagccagtaa caggcgacga gatagccgat gagagaaacc ggcagcgtga
     8641 ccagcgcctg cgccgttgcc cacatcatcg gatgtttgtc gggattttcc cgcagccagc
     8701 gggcgttgcg cagggcatac catgcccagc agaccacgga aactaacgcc agcacgatgc
     8761 cagaggtata acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca
     8821 cacacgccag gccgatgcca atacaaacca gtgccggggc gagttttccc cacgcgagtt
     8881 tgccgtcgcg ctggctataa agcagattgg caaaaacggg aatcaccacc ggcagggtgc
     8941 cgataatcat cgtggaaaca ggcgcaccag tacgttgaat ggcactggca aggcagaagt
     9001 aatagatgag gttgcccatc atagtgagca tcaaggcggt aagccagtcg cgacgcgcca
     9061 actgacgcag acgcacgcgt cccagccagg caatgggcag cgcaattaac cctaacgcca
     9121 gataacgccc catcgactgc aacatcgccg ggtattccgg tacgatcaac ggcccgacaa
     9181 aaataagccc ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg
     9241 gcatccattg atctgtcaga agaaacgttc aggaagcaga tatgattgct cccggcatcc
     9301 cgtcattatc agagaggatg cgggaggatg ccactttaat taactagtta ttaacattaa
     9361 aaagatggct tacgctttcg gctaacgttg tcgttgggcg gccaatcaat ttgctaagcg
     9421 ttttgctgtt atcaaacaga ccacctttcg atgcgccaac gtcagaatcc gccagcatat
     9481 ccgccagtcc gtcaggcagt ccgacgcttt tcagtgccgc ggcgaaatca gcttcactca
     9541 gattttgata ggtaacctgt ttgccgctct gtttggtcag ctctgccgct aactgtgtca
     9601 acgtccaggc actatcgccc gccagttcgt aaaccttgcc ttcgtgaccg gcttcgctaa
     9661 tcacgcgtgc cgcagctgcc gcataatctg cccgcgttgc tgaggcgatt ttgccatcgc
     9721 ccgccgcacc gataaatacg ccgtgttcca gtgctgccgg ggcgctggcg aggtagtttt
     9781 cgctgtacca gccgttgcgc agcagggtgt aaacgatgcc agaatcagcc aacattttct
     9841 ccgtctcaat gtgctcatcg gcgaggccga gcggagattt atccgcgtga agcagactgg
     9901 tataagcgat aaatttcaca ccagccgcct ttgcggcatt aataacatta cgatgctgcg
     9961 gggcacgttg acccacttcg ctggaagaga tcagcagtag tttttccact ccctgaagtg
    10021 cagatgtcag tgcggcttca tcgccgtagt cagcctgacg cacggtaatg ccttgtgctg
    10081 tcagtgcctg ggcttttgcc ggattacgaa cgatagccac tatttggctg gcaggaaccg
    10141 ttttcatcaa ggattcaata acatagtgac caagttggcc agtggcacca gtaattgcga
    10201 tcataggaag tctccatcgt ttgtcttgtg tttaaatacg ctaacaccta aacttacttt
    10261 tagtaagtac gtacaaaaag gtaagtatga gatgagtcag gttagcctgt cgcaacaact
    10321 gaaagaggga aatctcttcg ctgaacagtg tccgtcgcgc gaggtgttga aacacgtcac
    10381 cagccgttgg ggggtgttga ttctggtggc gctacgcgaa ggtactcatc gctttagcga
    10441 cctgcggcgc aaaatcggtg gggtgagtga aaagatgctt gcgcagtcgt tacaggcgtt
    10501 agaacaggat ggttttctta accgtatcgc gtatccggtg gtgccgccgc atgtggaata
    10561 tagcctcacg ccgctgggcg agcaggtgag cgaaaaggtt gctgcactgg cggactggat
    10621 tgagttgaat ttgcccgagg tgttagcggt gcgggatgaa cgtgcggcat aactgccgga
    10681 tgcgctgagc ttatctggtc tacggtgcat ggcttgtagg gcggataaga tgcgccagca
    10741 tcgcatccgc caataaatgc cggatgcggt tgatgatttg caggccgaaa aagaagtgta
    10801 ttacttactc aaatccacct gataaatcgc aaacccgata tcatcggtcg cgactttatt
    10861 catcggatat tgccctttat ctttaataaa cgctgcggct ttatctgacg gagaggtttc
    10921 gaaacggata tccagtttct tatcgccagt tatcggtgct aaacgccagt tgttatccgc
    10981 cgccgggtga atttcccccg cacgtttcga ctcttcagca atccacgctg ccagcaccga
    11041 gcggttctca tccggtgaag caaaagcgat atggctgtcg cccgtaccgg caaatttgcc
    11101 gccgtaagcg cgatagttat tggtggcaac gaggaacatg gcgttcggat caatcggctt
    11161 gccgttaaag atcaggttct taatcctttc cgcattggca ttaatcatct ggcactcgcc
    11221 gtcataacgg gcgggctggg taacatcaat ctgataattc acaccatcaa tcacatcaaa
    11281 gttataggtg cggaaaccat cccagttgat gagtgactgt ggtttcgtgc tgttaggatc
    11341 aatctggtta aactgtccgg cggagcactc cagccactct ttcacctctt taccgctggc
    11401 tttcaccaca atcagcgtat tgggatagag ataaagatcg gcggcattac ggaaagtcag
    11461 ctgacctttt tccacctcca catagcttgc cgggtcattt ttgcgaccac caactttaaa
    11521 cggtgcggca gctgaaagca ccggcagttt tgccagatcc ggatcgccct gaatgtaatg
    11581 ctcgacatac gctttttgcg cgttgttcac cacctgcacg gtcggatcgt cctgcaccag
    11641 cgccagatag ctatacatat tgtcggcaga tttaccgatt ggcttgctga cgaactggcg
    11701 tgtggcatcg tgatcggctt tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga
    11761 ttttttatta gcgatgtcgt aaatcggtcg tgcttccgct ttcgcctgcg tcacctgcca
    11821 tttaccgctg tcattactga gttgtaagtc gaccacacca agatgatcgc cccacatgcc
    11881 cggcattacc gccggaacac cattcagcgt gcctttggcg atatcagccc cttcgatatc
    11941 agcaaaatct ttacccggga aaacggcgtg agcatgacca aacataatgg cgttaacacc
    12001 cggaatttca ctgaggtaat aaactgagtt ttccgccatc actttatacg gatcggcaga
    12061 cagaccagaa tgcgccagaa cgacaacgac atcggcacct ttctcgcgca tttcaggcac
    12121 gtatttgcgc acggtttcgg taatatcatt caccgtcact ttcccggata aattagcttt
    12181 atcccagccc atgatttgtg gcgggacgac gccaatatag ccaatcttca gcgtctgttt
    12241 ttttccgtct ttatcgacca cttcggtgcc tttaattaaa tacggtgtaa acattggctg
    12301 tttggttctg gcgtcaatga cgttggcatt tacataaggg aatttcgctc ccgccagcgc
    12361 atttttcagg taatccagac cgtagttaaa ctcgtggttg ccaagcgtgc cgacggtata
    12421 gtccagcgta tttaatgcct tatagaccgg gtgaatatca cctgctttta aacctttcgc
    12481 cgacatgtaa tctgcaagcg gactcccctg aatcaaatcg ccgttatcga ccagtacgct
    12541 gtttttcact tcattgcggg catcgttaat caggcttgcg gtacgtacca gtccgaattt
    12601 ttccgtggcg gtgtctttgt aataatcgaa atccatcatg ttgctatgca gatcagtggt
    12661 ttccatgata cgcagatcga ccgtcgctgc attcacactg gcggcaatca gcgtggccag
    12721 gagcgttgcg ctaaacttaa tcatcaggga catcctttta tcatcgggaa tacgaaagaa
    12781 aagggagaat aaacgtctta cttatagaac agtgaagaat gccacaattt tacgctttga
    12841 aaatgatgac actatcacag ttggcgcatt cattaacgat agggtataag taaaacaata
    12901 agttaacacc gctcacagag acgaggtgga gaaatgttag atcaagtatg ccagcttgca
    12961 cggaatgcag gcgatgccat tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc
    13021 agcaaagcgg acaattctcc ggtaacggca gcggatattg ccgcacacac cgttatcatg
    13081 gacggtttac gtacgctgac accggatatt ccggtccttt ctgaagaaga tcctcccggt
    13141 tgggaagtcc gtcagcactg gcagcgttac tggctggtag atccgctgga tggtactaaa
    13201 gagtttatta aacgtaatgg cgaatttacc gttaacattg cgctcatcga ccacggcaaa
    13261 ccgattttag gtgtggtgta tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc
    13321 aaagcgtgga aagaagagtg cggcgtgcgc aagcagattc aggtccgtga tgcgcgcccg
    13381 ccgctggtgg ttatcagccg ttcccatgca gatgcggagc tgaaagagta tctgcaacag
    13441 cttggcgaac atcagaccac gtccatcggc tcttcgctga aattctgcct ggtggcggaa
    13501 gggcaggcgc agctgtaccc gcgcttcgga ccaacgaata tttgggacac cgccgctgga
    13561 catgctgtgg ccgcagctgc cggagcgcac gttcacgact ggcagggtaa accgctggat
    13621 tacactccgc gcgagtcgtt cctgaatccg gggttcagag tgtctattta ctaaattcag
    13681 atggcagaaa cagtgtattt cctgattctg ccatc
//
LOCUS       DAYPHN010000134        13680 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000134 DAYPHN010000000
VERSION     DAYPHN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.134"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..971
                     /locus_tag="ACG0BW_003601"
     CDS             <1..971
                     /locus_tag="ACG0BW_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7260523.1"
                     /translation="RRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHL
                     SLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSNFA
                     QIKMTDGTLNACSAMHVQFVNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVN
                     LFRADLSFSKLDLINFEHADLSRVNLNKATLQNINLIDSKLFFTRLTNTFLEMVICTG
                     SNMANVNFNNANLSNCHFNCSVLTKAWMFNTRLYRVNFEEANVQGMGITILREEENIP
                     INSDTLITLQKFFEEDCTSHTGMSQTEDNIHAVAMKITADIMRDAD"
     gene            complement(1125..2774)
                     /locus_tag="ACG0BW_003602"
     CDS             complement(1125..2774)
                     /locus_tag="ACG0BW_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFK7260524.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2925..4274)
                     /gene="ghxP"
                     /locus_tag="ACG0BW_003603"
                     /gene_synonym="yjcD"
     CDS             complement(2925..4274)
                     /gene="ghxP"
                     /locus_tag="ACG0BW_003603"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFK7260525.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4998..5462)
                     /gene="soxR"
                     /locus_tag="ACG0BW_003604"
     CDS             complement(4998..5462)
                     /gene="soxR"
                     /locus_tag="ACG0BW_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFK7260526.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5548..5871
                     /gene="soxS"
                     /locus_tag="ACG0BW_003605"
     CDS             5548..5871
                     /gene="soxS"
                     /locus_tag="ACG0BW_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFK7260527.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5877..7460)
                     /gene="pdeC"
                     /locus_tag="ACG0BW_003606"
     CDS             complement(5877..7460)
                     /gene="pdeC"
                     /locus_tag="ACG0BW_003606"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFK7260528.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQKNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRG"
     gene            7889..8170
                     /locus_tag="ACG0BW_003607"
     CDS             7889..8170
                     /locus_tag="ACG0BW_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFK7260529.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8269..8805)
                     /gene="ssb1"
                     /locus_tag="ACG0BW_003608"
     CDS             complement(8269..8805)
                     /gene="ssb1"
                     /locus_tag="ACG0BW_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFK7260530.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            9060..11882
                     /gene="uvrA"
                     /locus_tag="ACG0BW_003609"
     CDS             9060..11882
                     /gene="uvrA"
                     /locus_tag="ACG0BW_003609"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFK7260531.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11917..12273)
                     /locus_tag="ACG0BW_003610"
     CDS             complement(11917..12273)
                     /locus_tag="ACG0BW_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFK7260532.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12277..12693)
                     /locus_tag="ACG0BW_003611"
     CDS             complement(12277..12693)
                     /locus_tag="ACG0BW_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFK7260533.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12804..13517)
                     /gene="aphA"
                     /locus_tag="ACG0BW_003612"
     CDS             complement(12804..13517)
                     /gene="aphA"
                     /locus_tag="ACG0BW_003612"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFK7260534.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3599 a         3537 c         3310 g         3234 t
ORIGIN      
        1 cgagaagagt ttaccttaat aaagataagg aagtgtccat taaggtagta tattttataa
       61 acaatgtcac cgtccataat aatactatcg aaatcccaca gacagtaaat ggtggttacg
      121 atttttcaca ccttagcctg aaaggcatcg tgattaaaga tgaagattta tccaattcga
      181 attttgcagg ttgcagacta caaaacgcta ttttccagga ctgtaatatg tataaaacga
      241 attttaattt cgctataatg gaaaaaatac tttttgataa ttgtattctc gatgactcaa
      301 atttcgctca gataaaaatg actgacggaa ctctaaatgc atgctccgct atgcatgttc
      361 aattcgtcaa tgcaacaatg aatagagcca atattaaaaa taccttcctt gattattcaa
      421 atttttatat ggcgtacatg gctgaggtaa atctttataa agtaatagcg ccatatgtta
      481 atttatttag agccgatctt agtttctcta aactcgattt aattaacttt gaacatgctg
      541 atctgtctcg cgtcaatctg aacaaagcaa ccctccagaa tataaactta attgatagca
      601 aactcttttt tacgcggctg acaaatacat tccttgaaat ggttatatgt accggctcta
      661 atatggctaa tgttaatttt aataatgcca atttaagcaa ctgccatttc aactgttctg
      721 ttttaacaaa agcctggatg tttaataccc gtctctatcg ggttaatttc gaagaggcta
      781 acgtgcaggg aatgggcatt accattctcc gtgaggagga aaatatcccc attaatagtg
      841 ataccctgat aacactacag aaattctttg aagaagattg tacctctcat actggcatgt
      901 cacaaactga ggataatatt catgcagtcg ctatgaagat tactgcagat attatgcgag
      961 atgcagattg aggttaagcc gaagaacagc caatgaattg actttaaaag agagataact
     1021 tatttgtttt agaatcatgt atgttagccc gagctaacct gttaaaggtg caaaagattg
     1081 cacctttttc aaattacctc gggcctggtt acgtcgcttt cggcttactg attttcctca
     1141 atcaacaacg cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt ttcgttgctg
     1201 atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa tgccgccaga
     1261 cggaagcgac gctcaaggtt ctcttcctgc acggaacttt caacgtcgtt acgtccatcg
     1321 gcgcgacgac gcaggttacc aatgacgcga gaactgacct ccgtaagcag ctggttatcg
     1381 atattctctt cggtatccgc cgccagacgc tcctccattt tctggatcgc cacaatcgcc
     1441 acttctgccg ttgccgctcg cgcaatacgt tcctctttca attgttgcga atggtccgcg
     1501 acttcaatgt gttgtagcag aataggcaac atcaccacgc cgacaaacag cgagaagaga
     1561 atgacaccag ccgccaggaa taccagctcg tagcgcgccg ggaagacgtt accatccggc
     1621 aagagcagcg ggatagagag cacaccagcc agagtgatcg ccccacgcac cccggcgaaa
     1681 gacgcgatca ggatttctcg tgtggtccac gaaccaaact ccatcggctt cttcttcagg
     1741 aagcggttgc tgaacttttt catcgtccac agccagccga aacggaccag catcagcgcc
     1801 gcataaatca gaatgatatc ggtaaacagc atccagattt cgacgttagg gtcgatttct
     1861 gccgccatca gcgacgtctc cagaataccc ggcagctgca gacctaacag caggaatacc
     1921 atgccgttaa agacaaattc cagcatcgcc caggtactgt ttgcgcgcag gcgcattgcc
     1981 agcggcgcac ggcgcatcac accggagcgg gtgatggtca tcccggcggc aaccgcagcg
     2041 aggatgccgg aaacgccaat atgttcggca atcagataag aagcgaatgg cagcaagaac
     2101 agtagaacaa tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa tcgcagcgag
     2161 cgaccataca gccagctcac tacaaaaccg gcgagaatac cgccaatggc cactttcata
     2221 aattcgaccg tcgcaccgcc gacggtgaag atcatcgtcc ccatcgccac tgctacggca
     2281 aacttcaacg acaccaggcc agaggcgtca ttcatcaacg cttcgccctg caaaatgccc
     2341 atgatttttt tcgggatgcg cccttcgcca acaatcccgg agagcgccac agcatcggtc
     2401 ggagaaagca ccgccgccag cgcaaaggcg gggatcagcg gaatgcccgg caccacccag
     2461 taaataagga agccaatgcc gaccacggtg accaccacca gcgccagcgc gaggccgaaa
     2521 atctctcgac catgttcgag aaattcacgg gtcggcgttt tccagccatc agcgaacagc
     2581 aacggcggga taaacaagac taaaaagagt tcaggatcaa actccacatg caaaccaaac
     2641 gtcggccacg ccagtagcgc accgatggcg atttgcataa gcggaagcgg gatctgaaag
     2701 ggcatgacac gagtgaccac cccggacagc gagaccacga gggtcattat cagtatggtg
     2761 aagaagattt ccatgcgttc cctgtttgcg atgtttttta tgatctgaga aagacgttat
     2821 gcctgaattc tcattttttc agaatatcgc aaaagctcac acaatgtgcg gagaaaataa
     2881 aaacgggcag ccattgccac ccgttttcag aatcatatga aagattagat agcccagcca
     2941 cccgcataga aggtcaccag cgccacagcg ataacgaccg taccgatgtt caacttgcgc
     3001 cattcgccgg aaaccagacg accaatcacc agagtcgcga agccgatcat gatgcctgtt
     3061 acgatgttac aggtcagcac gatgaatacc gccgtaacca gacccgccat cgcatcaaca
     3121 aaatcagcaa agtcgatttt cgccacgtta ctcagcatca gcaggccaac gtacatcagc
     3181 gccggagccg ttgcataccc cggaacgagg taagagagcg gagagaggaa cagaatcagc
     3241 aggaacagca cgccaacggt gatagccgtc aacccggttt taccgcccgc cgccgtaccc
     3301 gccgcagact cgatgtatac cgctgccgga gctgcaccca ccaggccaga gaaaacgctg
     3361 ctcatggagt cggtggtcag tgctttccca ccgtcaatga tctgcccatc tttatccagc
     3421 aggttcgcct gaccggcgac ggcgcggata gttccggtgg catcaaatac tgccgtcatc
     3481 accagcgcca gaacgcttgg caggactaca ggattcagcg cgcccataat gtccaggcta
     3541 ccaatcagtg aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac gccggagaaa
     3601 tggacgttag gatcgaagat caaaccgaca attgagatac cgataatggt cagcagaatg
     3661 ccaccaggga ctttcagttt ttccaggccg atgatcaccg ccagacctac cagtgacata
     3721 atcaccggga aggtcgcgaa atcacccagc gcaacgggca gaccatcaag cgggttttta
     3781 atcaccagac cgacaccatt agcggcaatg agcagcagga acagaccgat accgatcccc
     3841 gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc agctacggat acccgtggca
     3901 gaaattaccg taaacagtac acccatcagg aacacagcac ccagcgcgac aggtacgcta
     3961 atatgttgcc ccagcaccag actgaatgcg gtaaacgccg tcagggagat ggcgcaacca
     4021 atcgccaacg gcagattagc ccacagaccc atcacgatag aaccgagtcc ggcaaccaga
     4081 caggtcgcaa cgaaaactgc cgcaggcggg aagcctgctt tacccaacat gcctggaacg
     4141 acgataaccg agtagaccat cgccagaaat gttgttaacc cggcaaccac ttcctgacgg
     4201 acagtgcttc cacgttgtga aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct
     4261 gatggcgtag acataagaaa acatcccctg agagtttaat tttcgtcaag atgcgtggcg
     4321 gacaatcccc tgccagcgaa acgttatctt cctgctccag gtttgcgaca aattggtgcg
     4381 tcgcaaaaaa gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa aaagcaaacg
     4441 attatccggc gatcatagcg gctttttcaa ccgaagtttg ctgctttttt ctcttttatt
     4501 gtcttgacga tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcgat gcgcaagatt
     4561 gcagcgttat cgagccatat accgttcagg attgcgataa aaaatcgtct gagatgacaa
     4621 aaatattgcg gctaaaaagt gtacttttac tctggcgaag aaacgccggt gacatacaat
     4681 taaagcatca acaccaaccg gaacctccac cacgtgctcg aatgaggtgt gttgacgtcg
     4741 ggggaaaccc tcctgtgtac cagcgggata gagagaaaga caaagaccgg aaaacaaact
     4801 aaagcgccct tgtggcgctt tagttttgtg tttgcgaata attccaaaag agctcgatca
     4861 gtcccctcgc cccttcgggg agagggttag ggtgagggga acaggcagca ccagtgcaaa
     4921 tatttaccct caccctaaag ggcgagggag aaaatcgccc ccatattaac gatgagtcag
     4981 cttgcccttg tggcgcttta gttttgttca tcttccagca agcgtgcgcc tgtgccttct
     5041 tctcctaagc ggtcgcccgg gttacgcaat gggcaatcac tgcgcgaaag gcagccacaa
     5101 ccaatacatc cgtccagttc gtcacgcagc gccactaagg tatgaatgcg ccgatccaac
     5161 tcttctcgcc attgggacga aagctgtttc cactctttcg cacttaatgt atgcccttcg
     5221 ggcaacacgc caaatgcttc acgaatggtc gctagcggaa tgccaatacg ctgagcaatt
     5281 ttgatgattg cgacatatcg caacacatca cgtttatatc gccgctgatt gccactatta
     5341 cggatactgc taatcaaccc tttgctttca tagaaatgca gcgccgatac cgccacaccg
     5401 ctgcgtttcg ccacttcgcc gggggttagc agcgctttaa tgcggggtaa tttcttttcc
     5461 ataaatcgct ttacctcaag ttaacttgag gaattatact ccccaacaga tgaattaacg
     5521 aactgaacac tgaaaagagg cagatttatg tcccatcaga aaattattca ggatcttatc
     5581 gcatggattg acgagcatat tgaccagccg cttaacattg atgtcgtcgc aaaaaaatca
     5641 ggctattcaa agtggtactt acagcggatg ttccgcacgg tgacgcatca gacacttggt
     5701 gattacattc gccaacgccg cctgttactg gccgccgttg agttgcgcac caccgagcgt
     5761 ccgatttttg atatcgcaat ggacctgggt tatgtctcgc agcagacctt ctcccgcgtt
     5821 ttccgtcggc agtttgatcg cactcccagc gattatcgcc accgcctgta gttttattac
     5881 ccgcgcgtta actcccgcgc gggtaattgc tccatccatt gcataaacac ctgcggcggc
     5941 atcgccttcg caaagaacca tccctggcaa tagcgcacgc cgcgtttgcg cagccagtta
     6001 acctgctcct cagtttcgac gccttcagcg atcgttttta atcccaggct gtgcgccagc
     6061 tcgatgatat gttccgcaat caaatgactg gttttatggg tggtcagcgt ctcaacaaac
     6121 gatttatcga ttttcaaaat atcgacattc aatgatttaa ggttatgcaa gttagagtag
     6181 ccaataccaa aatcatcaat tgccacttcg taaccagcct ggcggaaagc cagaataatc
     6241 ggcgtcattt tgtcgacatc aagaaatgca tgttcagtca cttcaaattt aatttgctgc
     6301 ggacgcaccg cgtattgctc tgttttctgg ttggttcgcg ctatcaaccg tgacgtatga
     6361 aaatcggagg ccgacaggtt aatagaaaca tagcgatcgg catgtgttgc caggtacgcg
     6421 cccagatcgc ggaagacatt atcaataaca taatcagtta cctgtccgat catcccctcc
     6481 ttttctgcca gcggaataaa ctctgccgga ttcattactt gcccctgctc accaggccaa
     6541 cgtaacaatg cttcagctcc gatacatttt tctgttttga tatcgattat tggctggtaa
     6601 taaaggcaaa gttgatgttt ttcgagggcg cgttgcaatt tgcgtttggg agacaaatag
     6661 ttttgtcgaa tacgtagcca gagtaataac agaacaagac tccccaaaat accggcgggt
     6721 aacgcaaaaa taagatgatt ataaaaatgc gctataagac gttgatatga agtcgcaaca
     6781 atggctgcaa ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttttttgt
     6841 acggttaaat cattcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctcgttgctt
     6901 aacgagaaaa aggttttcgt taccgtatca tacacacccc attgcaatgt cggatcatca
     6961 gacatcactt cactccagaa gagagggttg atgaccgcca cataatttcc ccgctgcata
     7021 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
     7081 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
     7141 gagcataaaa aatggttatc acgggcatag atcaattcat taatataaag atagccacga
     7201 ataatattca acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca
     7261 gcagcatcgc tcactaaatc tgcctggcga atgaccagct cggatttatc cagcgccagc
     7321 tgagcaaaag tttggagctg actattcact tctgattttg cccaaagaaa ggcaatccat
     7381 agcgacagaa tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg
     7441 tgtcgtgcac gatgactcat tgctatcaat ttcctgcctg tttatacccg gtcattgcaa
     7501 ggtgaaaacc ctggttaccg acgtctttag ataaatctta cggtataaat agcacttttg
     7561 cctgatttga aattgatacc tttttaatgg tcacctgaat aaaaataatt cattgatatt
     7621 caaattaatt ttctacagga atgcagaggc ggcgggaaga tatgccctta atgccactga
     7681 aaaaccacat aatctgtacg gcaataaaag tcacggccta atctggctaa caaacaatca
     7741 attgtgatat agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgtcctgc
     7801 aagccccgcg atagccagat tgtatcacgc ctcccgtgaa caacgccgcg ctgtccgagg
     7861 catcggctct tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct
     7921 tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt
     7981 tcgtcggtct gattatgtcg ggcttgcagt gatcagcagc ggtctgggcg tggtttctgc
     8041 ctgctggttc gcaatggggt tgcttggtat cacaatggcg gatatcaccg ccatctggca
     8101 caacatcgag tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat
     8161 tttgacttga tatatagaag cctccaaaac ggaggcttct tttttacggc tggcagatgc
     8221 tttaatcatc caccttaaaa caatataacc tattgtttta atgacaaatc agaacggaat
     8281 atcgtcatca aagtccatcg gcggctcgtt agacggtgct gccggagcag actgctgcgg
     8341 gcgagactgc gcgccgccgc tgaactgatt gccgccctgc ggctgctgag gctgacccca
     8401 accgccctgc ggctgaccac caccgatatt gccacctgca ggagcgccac caccctgacg
     8461 accacccaac atctgcatgg tgccgccaac gttcaccaca acttctgtgg tgtagcgatc
     8521 ctgaccagat tgatcggtcc atttacgggt acgcagctga ccttcgatat aaacctgaga
     8581 acctttacgc agatattcgc tggccacttc tgccagtttg ccgaacagca caacgcggtg
     8641 ccattcagtc tgctctttca tctcgccggt cgctttatca cgccaggatt cggaagtagc
     8701 cagcgtaatg ttggcaacag cgccaccatt tggcatgtag cgtacttccg ggtcctgacc
     8761 cagattacca acgagaataa ccttgtttac gcctctgctg gccatgttcg tgtctcctga
     8821 aaaaaaaacg ttctgaataa gtgtaaacgc gcgattgtac cattaccaat agcgctttta
     8881 ctatgttgtg acctcggttc cgggaaacaa acctggccag acattgttac acaacactcc
     8941 gggtaatgca ttccaatact gtatattcat tcaggtcaat ttgtgtcata attaaccgtt
     9001 tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg tttaatccgg gaaaggtgaa
     9061 tggataagat cgaagttcgg ggcgcccgca cccataatct caaaaacatc aacctcgtta
     9121 tcccccgcga caagctcatt gtcgtgaccg ggctttcggg ttctggcaaa tcctcgctcg
     9181 ctttcgacac cttatatgcc gaagggcagc gccgttacgt tgaatccctt tccgcctacg
     9241 cgcggcagtt tctgtcactg atggaaaagc cggacgtcga tcatattgag gggctttctc
     9301 ctgccatctc gattgagcag aaatcgacgt ctcataaccc gcgttctacg gtggggacaa
     9361 tcaccgaaat ccacgactat ttgcgtttgt tgttcgcccg cgtcggtgag ccgcgctgcc
     9421 cggaccacga tgtaccgctg gcggcgcaaa ccgtcagcca gatggtggat aacgtgctgt
     9481 cgcagccgga aggtaaacgc ctgatgctgc tcgcgccaat cattaaagag cgcaaaggcg
     9541 aacacaccaa aacgctggaa aatctggcaa gccagggtta catccgtgct cgtattgatg
     9601 gcgaagtctg cgatctttcc gatccgccga aactggaact gcaaaagaaa cacactattg
     9661 aagtggtggt tgatcgcttc aaggtgcgtg acgatctcac ccaacgtctg gcagagtcgt
     9721 ttgaaaccgc gctggagctt tccggtggta ccgcggtagt tgcggatatg gacgacccga
     9781 aagcggaaga gctgctattt tccgccaact tcgcctgccc aatttgcggc tatagtatgc
     9841 gtgaactgga accacgcctg ttttcgttta acaacccggc gggtgcctgc ccgacctgtg
     9901 acggccttgg cgtacagcaa tatttcgatc ctgatcgcgt gatccagaat ccggaactgt
     9961 cgctggctgg cggtgcgatc cgtggctggg atcgccgcaa cttctattac ttccagatgc
    10021 tgaaatcgct ggcagatcac tataagttcg acgtcgaagc gccatggggc agcctgagcg
    10081 cgaacgtgca taaagtggtg ttgtacggtt ctggcaaaga aaacattgaa ttcaaataca
    10141 tgaacgatcg tggcgatacc tccattcgtc gtcatccgtt cgaaggcgtg ctgcacaata
    10201 tggagcgccg ttataaagag acggaatcca gtgcggtacg tgaagaatta gccaagttta
    10261 tcagcaatcg tccgtgcgcc agctgcgaag ggacgcgtct gcgtcgggaa gcgcgccacg
    10321 tttatgtcga gaatacgccg ctacctgcta tctccgacat gagcattggt catgcgatgg
    10381 aattcttcaa caatctcaaa ctcgcaggtc agcgggcgaa gattgcagaa aaaatcctta
    10441 aagagatcgg cgatcgtctg aaattcctcg ttaacgtcgg cctgaattac ctgacacttt
    10501 cccgctcggc agaaacgctt tctggcggtg aagcacagcg tatccgtctg gcgagccaga
    10561 ttggtgcggg cctggttggc gttatgtacg tgctggacga gccgtctatc ggcctgcacc
    10621 agcgcgataa cgagcgcctg ttgggtacgc ttatccatct gcgcgatctc ggtaataccg
    10681 tgattgtggt ggagcacgac gaagacgcga ttcgcgccgc tgaccatgtg atcgacattg
    10741 gcccgggcgc aggtgtacac ggcggtgaag tggtcgcaga aggtccactg gaagcgatta
    10801 tggcagtgcc tgaatcgttg accgggcagt acatgagcgg taaacgcaag attgaagtgc
    10861 cgaagaaacg cgttccggcg aatccggaaa aagtgctgaa gctgacaggc gcacgcggta
    10921 ataacctgaa agacgtgacg ctgacgctgc cagtcggtct gtttacctgc atcacagggg
    10981 tttcaggttc cggtaaatcg acgctgatta acgacacact gttcccgatt gcccaacgcc
    11041 agttgaatgg tgcgaccatc gccgaaccgg caccgtatcg cgatattcag gggctggagc
    11101 atttcgataa agtgatcgat atcgaccaaa gcccaattgg tcgtactccg cgttctaacc
    11161 cggcgaccta taccggcgtg tttacgcctg tgcgcgaact gtttgcgggc gtaccggaat
    11221 cccgtgcgcg cggttatacg ccaggacgtt tcagctttaa cgtccgtggc ggacgctgcg
    11281 aagcctgtca gggcgacggt gtgatcaaag tggagatgca cttcctgccg gatatctacg
    11341 taccgtgcga ccagtgcaaa ggcaaacgct ataaccgtga aacgctggag attaagtaca
    11401 aaggcaaaac catccacgaa gtgctggata tgaccatcga agaggcgcgt gagttctttg
    11461 atgccgtacc tgcactggcg cgtaagctgc aaacgttgat ggatgttggc ctgacgtaca
    11521 ttcgcttggg gcagtccgca accacccttt ccggcggtga agcccagcgc gtgaagctgg
    11581 cgcgtgagct gtcaaaacgc ggcaccgggc agacactgta tattcttgat gagccgacca
    11641 ccggtttgca cttcgccgat attcagcaac tgctcgacgt gctgcataaa ctgcgcgatc
    11701 agggcaacac cattgtggtg attgaacaca atctcgacgt gatcaaaacc gctgactgga
    11761 ttgtcgacct gggaccggaa ggcggcagtg gcggcggcga gatcctcgtc tccggtacgc
    11821 cagaaaccgt cgcggagtgc gaagcttcgc atacggcacg cttcctcaag ccgatgctgt
    11881 aatcgttaag gccgctttct gagcggcctt ttcctttcag agttgcacca gcaatttacg
    11941 tttttcttcc ggcagcaaat tcaccgcctg ctgataagac gcatccacca gataatagat
    12001 ttgcgaatct ggcagcgaac cgtcgagata cacggtgctc cagtgcgctt tattcagatg
    12061 gcggcttgga cgcacatcgc tgtgctgctg acgtagcagc tccgccagct ccgggctggt
    12121 tttcagcgaa accgccgggc gattttctac ttctttcacc atcgcaaaca gtacatcttc
    12181 cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc tgttctgcgc ctggttttgc
    12241 catgcaatat tgtagcaact ccgaaatggt cattttttac tccccttgta gtgtcgcgat
    12301 aatgcgacgc gatccgccgt ggatgcgatg ttcccccagc cagatgcctt gccaggtgcc
    12361 ggtctgaata cgccctttat gcaccggcaa tacaagcgat gttcccagca ttgaggattt
    12421 gatatgagaa ggcatatcgt ctgctccctc atagtcatgc tcataatttc cgttgtcggg
    12481 aacggtgcgg aggaaaaaac gctccatgtc gtggcgtacg gtgggatcgc agttctcatt
    12541 aagtgtcaga gaggcggagg tatgttgcag caacagatgc agtaaaccga tgttaacgcg
    12601 cggcatatca gccagctgat tcagaatttc atccgttacc agatgaaacc cacgagattt
    12661 ggcgctaagc gtgagcgtct tttgatacca catgtgctgc tccttgataa aactctctta
    12721 atcagtttgc agcaagacag agaaaggata aaggtgtgat taaaaaaaca gcattcggga
    12781 gagtgtcacg ctctcccgct ctgtcagtat tctgaattga cgatcacctc ttcaccaaac
    12841 gcacccgctt gtggcaaggg tttgtaggta gagttggagg cgcgcagaat gcggatacca
    12901 cgagcgccga catcgcgtgc ggcggtaata tcgttatcgg aatcgccata aaaaattcgg
    12961 atatttttat cctgcagcca ttgcgatttt gtattttgcc ctggtttatc acccgcaaag
    13021 atcaccggat tcatgttggt ggcaggaata tgaaaattat ccgccagcgt ttttgaaacc
    13081 gtttctgttt tcgtcgggct acgaccagtc acaaagaaga tcgcgtcacc gcggcgtaca
    13141 tgcatatcaa tcagctggcg agcgacctct tttggaatgc tgaattcatc ccagccattg
    13201 ttcatttttt cccagaacac aggatttttc agataatctt cgctttctgg cgagaaggtt
    13261 tttttgccgc gccagaagcc cggactggaa aaaagtaccg tgtcatcgat atcaaacccc
    13321 accgccattg gcggacgccc tgcgaggcta ttttcaattt gtgcgaccga aacccaatga
    13381 attggtgcct gttcagcaag cctggcaacg ttagtaccag ggttaagcgg cgaaggagat
    13441 gaggccaggg caacagcgga actgtttaga gcgaacaata agcaaacggc actgattgcc
    13501 tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat gttgaaactt tttattgtga
    13561 gatttgttgc aaaaaccatc tgaccataac gccagcagca ggggaaagga aggattatct
    13621 gcggtttttg tgatgaaaag caggatgatt atatttaacc cagtaaatac gttcctgtcg
//
LOCUS       DAYPHN010000135        13539 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000135 DAYPHN010000000
VERSION     DAYPHN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.135"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            49..2277
                     /gene="crfC"
                     /locus_tag="ACG0BW_003613"
     CDS             49..2277
                     /gene="crfC"
                     /locus_tag="ACG0BW_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFK7260535.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQFAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQIKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQAVMTHSWIYIDAQLLRDDIQTLFTAE
                     RY"
     gene            2274..3152
                     /gene="rdcB"
                     /locus_tag="ACG0BW_003614"
     CDS             2274..3152
                     /gene="rdcB"
                     /locus_tag="ACG0BW_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFK7260536.1"
                     /translation="MIKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3416..4918
                     /gene="proP"
                     /locus_tag="ACG0BW_003615"
     CDS             3416..4918
                     /gene="proP"
                     /locus_tag="ACG0BW_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFK7260537.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5030..5119
                     /gene="pmrR"
                     /locus_tag="ACG0BW_003616"
     CDS             5030..5119
                     /gene="pmrR"
                     /locus_tag="ACG0BW_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFK7260538.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5095..6195)
                     /gene="pmrB"
                     /locus_tag="ACG0BW_003617"
     CDS             complement(5095..6195)
                     /gene="pmrB"
                     /locus_tag="ACG0BW_003617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFK7260539.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTNTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6196..6864)
                     /gene="basR"
                     /locus_tag="ACG0BW_003618"
     CDS             complement(6196..6864)
                     /gene="basR"
                     /locus_tag="ACG0BW_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFK7260540.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6861..8504)
                     /gene="eptA"
                     /locus_tag="ACG0BW_003619"
     CDS             complement(6861..8504)
                     /gene="eptA"
                     /locus_tag="ACG0BW_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFK7260541.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNETRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8608..9945)
                     /gene="adiC"
                     /locus_tag="ACG0BW_003620"
     CDS             complement(8608..9945)
                     /gene="adiC"
                     /locus_tag="ACG0BW_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFK7260542.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10082..10843)
                     /gene="adiY"
                     /locus_tag="ACG0BW_003621"
     CDS             complement(10082..10843)
                     /gene="adiY"
                     /locus_tag="ACG0BW_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFK7260543.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11179..13446)
                     /gene="adiA"
                     /locus_tag="ACG0BW_003622"
     CDS             complement(11179..13446)
                     /gene="adiA"
                     /locus_tag="ACG0BW_003622"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFK7260544.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3254 a         3316 c         3572 g         3397 t
ORIGIN      
        1 atctggctaa ataaaagtaa caaaatttgc tttaaggaag aattttctat gtacacacag
       61 accctgtatg agttaagtca ggaggctgaa cgcctgttac agctttctcg ccaacagttg
      121 cagttactgg aaaaaatgcc tctctctgtc cccggagacg atgcgccaca actggcttta
      181 ccctggagtc agcctaatat cgccgaacgt cacgcgatgc tgaataatga gttgcgtaaa
      241 atttcccgac tggaaatggt gctggcgatt gtcggtacca tgaaagcagg gaaatcaacc
      301 accattaatg ccattgttgg tacggaggtt ttgcctaatc gtaatcgccc aatgactgcg
      361 ctgccgacgc ttattcgcca tacgcccggg caaaaggaac cggtactgca tttttcacat
      421 gtcgcgccaa tcgattgttt aattcaaaaa ttacaacagc gcctgcgtga ttgcgatatt
      481 aagcatctga ccgatgtgct ggaaatagat aaagatatgc gtgcgcttat gcagcggatc
      541 gaaaatggcg tcgctttcga aaaatattat ctgggtgccc agcctatttt tcattgtctg
      601 aaaagtttga atgatttggt gcgactggcg aaggcgctgg acgtcgattt tcctttttct
      661 gcttacgccg ccattgagca tattcccgtg attgaagtgg agtttgtcca tctggcgggg
      721 ctggagagtt atcccggtca attgacgtta ctggataccc ccgggccaaa tgaagccggg
      781 caaccgcatc tgcaaaaaat gcttaaccag cagctggcac gcgcctcggc ggtactggcg
      841 gtgctggatt atacgcaact gaaatcgatc tccgatgaag aggtccgtga ggcgattttg
      901 gcggtggggc aatcggtgcc gctgtatgtg ctggtcaata agttcgatca acaggatcgt
      961 aacagtgacg acgccgacca ggtgcgggca ctgatttccg ggacgctgat gaaaggctgt
     1021 attacgccac agcagatatt tccggtgtcg tcgatgtggg gctacctggc gaatcgggcg
     1081 cgccatgagt tagccaacaa cggtaagtta ccaccgccag agcaacaacg ctgggtggaa
     1141 gattttgccc atgccgcgct cggcaggcgc tggcgtcatg ccgatctggc tgacctcgaa
     1201 catattcgtc atgctgccga tcagttgtgg gaagattcgc tgttcgccca gccaattcag
     1261 gcgttgcttc atgccgctta cgctaacgcc tcgttgtatg ctctgcgatc tgccgcgcat
     1321 aaactgttga attacgcgca gcaggcgcgg gaatacctgg attttcgtgc gcacgggtta
     1381 aacgtcgctt gtgaacaatt gcggcaaaat atccatcagg tcgaagaaag tttgcagcta
     1441 ttgcaactca atcaggcgca ggtgagcggc gagattaaac atgaaatcga gctggccctg
     1501 acctccgcca accactttct gcgtcaacag caagatgcgc tgaatgccca gtttgccgcc
     1561 ttgtttcagg atgattcgga gccgttaagc gagattcgta cccgctgtga gacactgtta
     1621 cagacggcgc agaacaccat cagtcgcgac tttacgctgc gttttgccga gctggaatcc
     1681 accctttgcc gggtgttaac cgatgttatt cgccccattg agcaacaaat caaaatggaa
     1741 ttgagcgagt cagggtttcg tcctgggttt cattttcctg tttttcacgg cgcagttccc
     1801 cacttcaaca ctcgccagct gttcagtgaa gtcatttcgc gccaggaagc aacggacgag
     1861 cagagcacgc gtttaggcgt tgtgcgtgag actttttcgc gctggttgaa tcagcccgac
     1921 tggggacggg gaaatgagaa atccccgaca gagacggttg attacagtgt gttgcaacga
     1981 gcattaagcg cagaagtcga tctttattgc caacaaatgg ctaaagtttt ggcagagcag
     2041 gtcgatgaat ctgttacggc aggcatgaat acttttttcg ctgagttcgc ttcatgtttg
     2101 acggaattac agacgcgttt acgcgaaagc ctggctctgc gtcaacaaaa tgaatcggtg
     2161 gtcaggctga tgcagcagca attgcagcag gctgtgatga ctcacagctg gatttacatc
     2221 gacgctcagc tgttacgcga tgatattcaa acacttttca cggcagaacg atattgatca
     2281 agacgttact ggacggcccc ggtcgcgtgc tggagtcggt tcatccgcgc tttttagtgg
     2341 atctggcgca gggcgatgat gcccgccatc cacaagccca tcagcagcag tttcgtgaac
     2401 gactgatgca ggaacttctt gcgcgtgtgc agcttcaggc atggacgaac ggcgggatgt
     2461 taaatgcgcc gcttagcctg cgtctgacat tggtggaaaa actggcgtcg atgctggatc
     2521 ccggtcatct ggcgctgacg cagatcgcgc aacatctggc gctgctgcaa aaaatggatc
     2581 accgccagca ctctgctttc ccggagctcc cccagcaaat tgccgccttg tatgagtggt
     2641 tttcagcccg ttgtcgctgg aaggaaaagg cgttaacgca acgaggccta ctggtgcagg
     2701 caggtgatca gagcgagcaa atttttaccc gctggcgtgc tggggcgtat aacgcctggt
     2761 cgttgcctgg gcgctgtttt atcgttctgg aggagttgcg ctggggggca tttggcgatg
     2821 cctgccgtct gggaagcccg caagcggtgg cgttgttgct gggtgatttg cgcgagaaag
     2881 cgacacaaca tctggcagag agtatcaatg cggcaccgac cacgcgtcac tattaccatc
     2941 agtggtttgc ctcttcgacc attccgacgg gcggggatca tgctgatttt ttaagttggc
     3001 tgggaaagtg gaccacggca gataaacaac ccgtttgctg gtcagtgacc caacgctggc
     3061 aaactgtcgc gctggggatg ccacgactct gttcagcgca gcgtctggcg ggggcaatgg
     3121 tcgaggaaat cttctctgta aatttggtgt aaataatcag ttacatcaat gagtcctaaa
     3181 cgaaatccat gtgtgaagtt gatcacaaat ttaaacactg gtagggtaaa aaggtcatta
     3241 actgcccaat tcaggcgtca actggtttga ttgtacattc cttaaccgga gggtgtaagc
     3301 aaacccgcta cgcttgttac agagattgca tcctgcaatt cccgctcccc ttttgcggcc
     3361 gtcgcgctga tttttctggc gtttgcggaa atgggccaac tctgcgagga aagctatgct
     3421 gaaaaggaaa aaagtaaaac caattaccct tcgtgatgtc actattattg atgacggtaa
     3481 actgcgtaaa gccattaccg cagcatcact gggtaatgca atggaatggt tcgattttgg
     3541 tgtttatggt tttgttgctt acgcattagg taaagttttt ttcccggggg ctgaccccag
     3601 cgtgcagatg gttgctgcac ttgccacttt ctccgttccc tttctgattc gaccgcttgg
     3661 tggactcttc tttggtatgt tgggcgataa atatggtcgc cagaagatcc tcgctatcac
     3721 tattgtgatt atgtcgatca gtacgttctg tattggctta ataccgtcct acgacacgat
     3781 tggtatttgg gcaccgattc tgctgttgat ctgtaagatg gcacaaggtt tctcggtcgg
     3841 cggtgaatat accggggcgt cgatatttgt tgcggaatac tcccctgacc gtaaacgtgg
     3901 ctttatgggc agctggctgg acttcggttc tattgccggg tttgtgctgg gtgcgggcgt
     3961 ggtggtgtta atttcgacca ttgtcggcga agcgaacttc ctcgactggg gctggcgtat
     4021 tccgttcttt attgctctgc cgttagggat tatcgggctt tacctgcgcc atgcgctgga
     4081 agagactccg gcgttccagc agcatgttga taaactggag cagggcgacc gcgaaggttt
     4141 gcaggatggc ccgaaagtct cgtttaaaga gattgccact aaatactggc gcagcctgtt
     4201 gacatgtatt ggtctggtaa ttgccaccaa cgtgacttac tacatgttgc tgacctatat
     4261 gccgagttat ttgtcgcata acctgcatta ctccgaagac cacggggtgc tgattattat
     4321 cgccattatg atcggtatgc tgtttgtcca gccggtgatg ggcttgctga gtgaccgttt
     4381 tggccgtcgt ccgtttgtgc tacttggtag tgttgcactg tttgtgttgg cgatcccggc
     4441 gtttattctg attaacagta acgtcatcgg cctgattttt gccgggttac tgatgctggc
     4501 ggtgatcctt aactgcttta cgggcgttat ggcctctacc ttgccagcga tgttcccgac
     4561 gcatatccgt tacagcgcgc tggcggcggc atttaatatt tcggtgctgg ttgccggtct
     4621 gacgccaacg ctggcggcct ggctggtcga aagctcgcag aatttgatga tgcctgccta
     4681 ttacctgatg gtagtggcgg tggttggttt aatcaccggc gtaaccatga aagagacggc
     4741 gaatcgtccg ttgaaaggtg cgacaccggc ggcgtcagat atacaggaag cgaaggaaat
     4801 tctcgtcgag cattacgata atatcgagca gaaaatcgat gatattgacc acgagattgc
     4861 cgatttgcag gcgaaacgta ctcgcctggt gcagcaacat ccgcgaattg atgaataagc
     4921 tgaaacggat ggcctgatgt gacgctgtct tatcaggcca attgaagtct taaggttcac
     4981 ttaatctctg acgcgcatac tctcctccag gttaacggag gagagtgcaa tgaaaaaccg
     5041 tgtttatgaa agtttaacta ccgtgttcag cgtgctggtg gtcagcagct ttctttatat
     5101 ctggtttgcc acgtactgat ctttcttcag ccgtacccag gcccgcgtgc cggaagtctc
     5161 ttgccggttt tgcaggaaaa actgcccgtg atgcaactgt gtaatgcggc tgacaatact
     5221 taaccccaga ccaatcccgc cataacggct gtccatacgt acaaatgctt tactcaactc
     5281 cccgcattta ctctcatcaa tacccggtcc ttcatcttca acagccatga ccgctccgcc
     5341 atcttcttgc agcttaatca taatgttgct gccttgcggg ctgtaacgat gggcgttttc
     5401 taccaggttt cgcaataaca tccgcagcag ggttgcatca ccctgaacgg tgatgtcggc
     5461 ggcgctctct ggcaatagca gggtttgctg tcgctggtcg agcatggtac tgagttcgtc
     5521 atacgagggg agaatgacat cttccagcag ttttacatgt tgataattac cggaagaaaa
     5581 tgactgtccg gcacgcgcca gttgcagcag ctgggagacg ctctccatca tctgatcaag
     5641 ccgtgccact aacggtgcta catcaatgtg atgcgttttc gccagcagtt ccagatgcaa
     5701 acgcaccccc gccagtggcg ttcgcagttc gtgcgcgacg tcagcggtaa acaacctttc
     5761 gttatccagc gtgttggtca ggcgactgac cagatcgttt aacgccgaaa ccaccgcttc
     5821 gatttcgagg gtggcgctgt gaatggcaat gggcgttaag ttgtcggcgg tgcgcgcttc
     5881 cagctctttt tgcagctccg ccagcgggcg ggtgatgcgg cgtaccgcct gatagcagat
     5941 aaatagcgtc aggctgacca taaagacgcc ggggacgatc aggctggcga ccgcctcgcg
     6001 gatctcacgc atgatgtggc gatcgttgtt gcgattgtcg cgtagcgcct gctcaaacag
     6061 ctgaatctgc tcggtacttt catgccatag ccagaagacg ctgatcagct caaacaccaa
     6121 caaaatggcc ccaatggtca atatcagccg ttggcgcagc gatattggtc ggcgcagaaa
     6181 acgcatcaga ttcaattagt tttcctcatt cgcgaccagc atatagccaa atccgcgcac
     6241 ggtgcggata cgggctttgc ccactttgtc gcgcagattg tggatatgca cttccagggt
     6301 gttagtcgag ggttcattgt cccagttata gatgtcgttg tagagaattt cccgatgcac
     6361 cggactgcct gctttgagca ttaaccgtga cagcagagca tattctttgg gcgtcagaat
     6421 caactcttca ccgcccatcc atacctgacg gcgacccata ttcagcgtca gattgccaac
     6481 aatcagctca ctttcgccct gattattatg gcgtcgtagc agggcgcgga tacgggcgtg
     6541 taactcttcc agcgcaaaag gcttcaccag atagtcatcg gcaccgacat ccagcccggc
     6601 gattttgtcg gtcagcgtat cgcgagcggt gaggatcagt accggcaggg tgtatttttt
     6661 ctgccggata cgggcgagaa aatgcagtcc atcttcgtcg ggtaacccta aatccagtac
     6721 caccaggctg taatgaccgg cctcaaggct ttgttccgcc atccgcgcgg ttgtcacgcc
     6781 atcgcacgcg tagccttcgg tttgcgccgc cagaatcaat ccctgcaata acagcgtatc
     6841 gtcttcaaca atcagaattt tcattcactc actctcctgc aagtttgcag aatatcatcc
     6901 gcagcctggt aatacttcgt ctcaacgcca gttaatccca atagcgtgga gaataaatta
     6961 tcttgtgaat agtgttgcgt ttgcgcctgt ttttgcaggc agttctggtc aacctgatac
     7021 cgtttttgat aatcctccga cagccacagc agcattggca cctgtttttg gctatccggg
     7081 gcgatggcat aaggcagacc gtgcagatag atgccatttt cacctaacga ttcaccgtgg
     7141 tcagaaagat aaaccaggct ggtggtaaat ttatcctgat gttctttcag cagattaatc
     7201 gctttatcaa caatatagtc gacgtaaacc agcgtgttgt cgtaagtgtt caccagttgc
     7261 tctttggtac aggtctggat ctcgttagtg tcgcaggttg gggtaaattt cctgaactgc
     7321 ggcggatagc ggttgtaata ggtcggaccg tggctgccga tggtgtgtaa gacaatcacg
     7381 ccatcaccct gtaggttatt gatgtactct tccagcccgt gaaacagcac ttcgtcatag
     7441 cattcgccgt tgatgcactg accaggcaga ttcagcgcgg tgacgttctg gtgaggcacg
     7501 cggtcgcagg cacctttaca gccgccatcg ttgtcattcc acagcacgtt gatgcccgct
     7561 cgctgaatga tatccagcac gccttcctgg tgctgtgcca gctcttcttt gtaatgctca
     7621 cgcggcatat ccgagaacat gcacggtact gaaactgccg ttgccgtgcc gcaagatgcg
     7681 gtattaggga aatagaccac gttatctttc gccagccgcg ggttagtttc acgcgggtag
     7741 ccgttgaggg agaagttctc cgcccgcgag gtttcgccga caatcaggat ggtcaaattt
     7801 ttacgtgtct cgttctgcat taatggattg cggtgcgcgt cttctccaat tcgcaccagc
     7861 ggcagatttg ccagtcgctg atgggagtac catgaccagc tggcaacaat gctattagag
     7921 gggcttaagg acttcaccag ctctttgtta ttgcggaaca atgaggcgta gtctttataa
     7981 aacagtgcgg cgaccagcaa aatcagcagt acagaaacca gaatattggc tccacggaaa
     8041 agaacactgc gcaggcgcga ggtggctggt ttgattttaa tccagcaggc aatcagcgca
     8101 gcaagcacgc cgctgaatcc cagcgttaat aacatttgcg gtgtcatcaa cgcataactt
     8161 tctgccggag tggtatcaat aatattggct atcatcgagc ggtcgatgac gatgccgtaa
     8221 gtcattatga aatattgtgc agctgcgccg accagaataa acaggcaggc cagcggtcga
     8281 ttaagccata agaaagagct tagtgtcagg acaatattaa tcacgctgaa ggcgacgacc
     8341 ggcatcgaca agaaaaccag tacgttatgc agcgagtcca gcggcagcgc ctgcaacacc
     8401 tgtttaaaaa aggcaatatt caggcagata gagatataaa aagcggccaa caatagccag
     8461 gcgagtaaat tcaaagaggg tctttttagt aggcgcttca acatcacggt gtttccatcg
     8521 aacaaagtgc gcatatgctc gcaaagcaaa attaagccaa ccttaagttc ttaaggttgg
     8581 cttttatgtt tgcttgatta aggggaatta atctttgctt attggtgcat ctaagggata
     8641 cgggttttta tgcagccggt tgtaattcag cgcatacatg gcggtgatga ccatcagggt
     8701 gacaaatgac cacataacct ctttcgcccc ggacccgacc acggcccaga tgcagtagag
     8761 gaaggcaatg gtagtaactg ccaggtatgc cgggcgtgct ttaccaaagt gaccgtggcc
     8821 gagcagcagt aatgccgcac aggtgtaaag atatggcacc agtgtaaaga tgaccgagac
     8881 ggaagaaacc agaccgaact ctttggtcgc gtttggcgaa atgctgctga gctggaagat
     8941 ggtcatcaaa ataccgacga taatcagccc cgccactggt gtgcccgctt tattaacacg
     9001 ggcaaaaatt ggtgggaaca gtccgtcatc ggcagcggct ttcgccgttt gacccgccag
     9061 caatgtccag ccgcccagtg aacctaagca acccgcagct gcgcagaagg aaacaatggc
     9121 cccggcggtg tcacccaacg ccatccgtgc ggcatcaccg aatggcgagg cagaaacgcg
     9181 cagtgcggca ttcgggatca tccccataat cgcggtggtg gagagcacat agcaaacggc
     9241 ggcaatcaat accccaccaa tggtggcgat agggacgttg cgtttcgggt ttttcaccac
     9301 acctgcggca acggaggcac tttccacacc gatgaacgac cacagcgtaa cgttaagggt
     9361 actttgaatt gcaccgaagg tgcccaggcc gctgacgttc catgccgcca tataggtttc
     9421 accacggaac cagaaccagc caaatacggc aatcccgacg atggggatca gcgccagcac
     9481 agtggcaacg gcctgcacgc gggtgatcat tttcggaccg acaatattca gcaggacgaa
     9541 gatccacagc accacgacgc aggtgatggt taataccagt ggatctttca gaatcgggaa
     9601 gaagtaactt aaataaccta cgccaatgac caccatggcg atattgccga tccagcaggc
     9661 cagccagtag aggacgttgg tttgataacc gagaaacggc ccaaagcagc ggcgggcgta
     9721 agcgtaagaa ccaccaggac ttgggtcgag gaacgacatt ttggcgtata ccatcgagag
     9781 ccccagcgca ccgataatcg tcaccaacca tccataaatg gcaatcccgc cagtggaggc
     9841 caggtttgca ggtaacagaa aaacacctga ccccataata ttccccgaca ccatcagggt
     9901 aacggggatt aagcccactt tgtgagcatc agcatccgaa gacataatta aactcctgcg
     9961 aaggcgagct tcgtgacaat aaattacgtc atatcatacg cctgcattcg atagttgaat
    10021 tattatcgac cgggttatcg ctgatgccgt tattaatata gctcttcgtt aaggttaaaa
    10081 atcaggcgac agtgcgttct ctgtgctgac taacataatg cagtggcgtc ataccgtaga
    10141 agtctttaaa gacagaaata aagtacgacg tactgttgta gccgcaggac tgtgatacct
    10201 gagagatatt tttaccgtcc atcattaatt catttacggc ataacgcatg cggcaggtgg
    10261 tgattatttg gctataactg gtgttttcgc ttttcagctt tttctttaac agactcgggc
    10321 taagacataa acaactggct accatactaa gattccagtc tttgtggata tcgctttcaa
    10381 taatttgata aacgctgtca cttacacaat tacgtaacat atacatcatt agtgaaagga
    10441 attttttact gtcgagaaaa cgggatagca cagtgaataa taatgcacgt gttcgttcct
    10501 tttctgactc agtttcagcg ggcatcatgc tgtgttgcgc ggcaacgcga aagacgtctg
    10561 gcgtcaggca tggcagagtg aggatgggcg tagcgctacg ttgccatact ggtatttgcg
    10621 agagatcttt attcagaaac cggaggtaat ctttgatgat gtcgtgacta atatgagcaa
    10681 ccaaagtgtt attaagagag gagacgtcga taatattatt ttcacaattt aataacgcca
    10741 tatggttagc tttaaggcta ataggttctt tcccattcac ccttatccag acatcttttt
    10801 cagtcaataa aacaatacaa ggttggtcgc tgcaaatcct catacatgta ctcctgagtg
    10861 cgaataaaag accagttctc gctaaagcga agcgataccg ggaaatggtt atataattgt
    10921 atgatgaata taaacatgca taaaaataac gagttttact aatattacaa atttttatag
    10981 taatttctca tgtacatatt tttttacagg ctaaaaaaag gataaataac aggcaaaaaa
    11041 aagcgcgggg tgggaccgcg caaaaagcat gacatttttt gtttgcagca atttttatga
    11101 aaacgttgcc atacttaaaa ggcctcaagc caggcggcgt aatgttattt aacaattacg
    11161 ccttcagcgg aatagtggtt acgctttcac acacataacg tggtaaatac cgtcaataat
    11221 ttctgtccct tctgtttcgt gttcaaaccc agggaaatga tggtcccagg attgcagcga
    11281 gcgtaaataa cttacttgcg gactgttttt atcgccgaag ttttcaccag acagcagcat
    11341 cgggattcct ggcggatacg ggataactga gtttgccgcg atgcgtcctg gcagattttc
    11401 aatggatacc agttcgacat tgttgtcgac aatcgcgttg tacgcttcac gtggggtgat
    11461 ttccgccacc ggcaggccgg aataggcttc gttcaaccgt gcgccagggt tgttttcttt
    11521 cagccaggca aacatggtgt cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg
    11581 atattgttca acaagttccg gcatcacctg cgccagcggg gtgttggcgt catagtggcg
    11641 tttgaaggag caaagggtgt taaccagagt cccccatttc ccacgggtca cgcccataga
    11701 gaacaggaac ataatctgga agtcagtggt acgggtaggc acaatgccgt ggcgaccaag
    11761 ccaggctgtg accagcgccg ccggaacacc ggtttcttcc agttcaccat cttcacccat
    11821 tcccggagca aggatgctga ctttaatcgg gtcgagcata ctccagttat ccggaatatc
    11881 tttgaagccg tgccagcttt cgcccggatg catgacccag cagtcctgaa cggtggtcag
    11941 cagtttggtt ggtgcgtcag caaagtcata ggttttgccg gtttgtgggt cggtgacgac
    12001 ttctttgttc cacggtttga agaaccagct accgtcagcg gtgaactctt tatacagccg
    12061 cgccatcgcc tgacggaaat caaccgcttc gtcgatcact tcctgcgtca gtgacaggcc
    12121 gctgttgccg tccatcatgg acaccgccac gtcgttggat gcgcagatgg catacagcgg
    12181 ggaggtggtg gcatgcatca tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg
    12241 accttcacgt acatgaatat aagaggcctg tgacagcgca ttcagcagtt tgtgggtgga
    12301 gtgggtggcg aaaacggtag gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg
    12361 atcggcatag atcgggttga aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg
    12421 atcggaggtt ttttccagca gatcctgcgc ttctttagcg ttataacaca cgccgtcata
    12481 ggtgcagttg gtcaccacgc agtaagacgg tttttgcccg gctttgtctt tagtcagcgg
    12541 gctttcactg attttcttct gcaaggtttc aggttgcatt tcctgcggat agattggccc
    12601 gataatgccg tagcggttgc ggcttggcac catgtagacc ggtttcgcgc ctgtcagcat
    12661 caagccttgt tcgatggatt tatggcagtt acggtcaacg accacgacat cgttatcggt
    12721 catgcaagcc tgcatgatgg tgcggttaga gccggaagta ccgacgacta ccgaccagga
    12781 gcgatcggca ccaaatacgc gtgcggcata tttttcgctt tcgccaaatg cgccagtatg
    12841 gtcaagcaaa gaaccgaggg aagtgcgttc gatgcccatg tcggtgcgga acagattttc
    12901 accatagtag tcatggtaga aacgtccggc gggtgtttta gtaaaaccaa cgccgccctg
    12961 gtggccaggt gctgcccagg aatattcatg gatgtcgcta tatttcatca gcgcgctgaa
    13021 cagtggcggc aacagctgct ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat
    13081 aaagtcggcg gtatcttcca gaatccaggc gaattcatcg acaagctcca gcagatcccg
    13141 gtccattgcg gcgagggctt tttcccgatc gcccaacagg aagaccggca cgttttgttg
    13201 gcgctcatga agcttaccga tcaattgtct gacgttttga tgttcgtccg gatgttccat
    13261 ttgatagctg aacatcaggc agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc
    13321 atcaaaggag gtggatttaa tcacggtaac attttgctgg cttaaagcat ctgccagacg
    13381 ctcaacggcg ttaccgaccc aggtgtcttg atggagaaac tcgctttcaa caattaatac
    13441 tttcatcatt gcttacccgg ttatgaagga atcttcgttg cgggcaagta tcttccggcc
    13501 ttttcgggct gtaaagcgcg tgaaaaacaa acatttttg
//
LOCUS       DAYPHN010000136        13286 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000136 DAYPHN010000000
VERSION     DAYPHN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.136"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            190..1284
                     /gene="gcvT"
                     /locus_tag="ACG0BW_003623"
     CDS             190..1284
                     /gene="gcvT"
                     /locus_tag="ACG0BW_003623"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFK7260545.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFSEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1308..1697
                     /gene="gcvH"
                     /locus_tag="ACG0BW_003624"
     CDS             1308..1697
                     /gene="gcvH"
                     /locus_tag="ACG0BW_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFK7260546.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1816..4689
                     /gene="gcvP"
                     /locus_tag="ACG0BW_003625"
     CDS             1816..4689
                     /gene="gcvP"
                     /locus_tag="ACG0BW_003625"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFK7260547.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWSEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4855..6288)
                     /gene="bglA"
                     /locus_tag="ACG0BW_003626"
     CDS             complement(4855..6288)
                     /gene="bglA"
                     /locus_tag="ACG0BW_003626"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFK7260548.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            6333..6644
                     /gene="yqfB"
                     /locus_tag="ACG0BW_003627"
     CDS             6333..6644
                     /gene="yqfB"
                     /locus_tag="ACG0BW_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFK7260549.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6808..7467
                     /locus_tag="ACG0BW_003628"
     CDS             6808..7467
                     /locus_tag="ACG0BW_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFK7260550.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7544..8524)
                     /gene="ygfZ"
                     /locus_tag="ACG0BW_003629"
     CDS             complement(7544..8524)
                     /gene="ygfZ"
                     /locus_tag="ACG0BW_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFK7260551.1"
                     /translation="MAFTPFPPRQPTASARLPLTLITLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8767..9033
                     /gene="sdhE"
                     /locus_tag="ACG0BW_003630"
     CDS             8767..9033
                     /gene="sdhE"
                     /locus_tag="ACG0BW_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFK7260552.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9014..9421
                     /locus_tag="ACG0BW_003631"
     CDS             9014..9421
                     /locus_tag="ACG0BW_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFK7260553.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGKEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9461..9982)
                     /gene="fldB"
                     /locus_tag="ACG0BW_003632"
     CDS             complement(9461..9982)
                     /gene="fldB"
                     /locus_tag="ACG0BW_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFK7260554.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10094..10990
                     /gene="xerD"
                     /locus_tag="ACG0BW_003633"
     CDS             10094..10990
                     /gene="xerD"
                     /locus_tag="ACG0BW_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFK7260555.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     STLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11015..11725
                     /gene="dsbC"
                     /locus_tag="ACG0BW_003634"
     CDS             11015..11725
                     /gene="dsbC"
                     /locus_tag="ACG0BW_003634"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFK7260556.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            11731..>13286
                     /gene="recJ"
                     /locus_tag="ACG0BW_003635"
     CDS             11731..>13286
                     /gene="recJ"
                     /locus_tag="ACG0BW_003635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFK7260557.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKV"
BASE COUNT         3080 a         3368 c         3643 g         3195 t
ORIGIN      
        1 ttggcgtttt ttaaccataa gctaatgtga tgatcaattt taccttatgg ttaacagtct
       61 gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg agtggctccg
      121 atgctgggtt tcgtggtgat aatttcgcca tggaaaagtt gtcagccccg cttattcaat
      181 gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg cggcgctcgc
      241 atggtggatt tccacggctg gatgatgccg ctgcattacg gttcgcaaat cgacgaacat
      301 catgcggtac gtaccgatgc cggaatgttt gatgtgtcac atatgaccat cgtcgatctt
      361 cgcggcagcc gcactcggga gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc
      421 accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg tgtgatagat
      481 gatctcatcg tctactactt ttctgaagat ttcttccgcc tcgttgttaa ctccgccacc
      541 cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat cgaaattacc
      601 gttcgtgatg acctttccat gattgccgtg caagggccga atgcgcaggc aaaagctgcc
      661 acactgttta atgacgctca gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg
      721 caggcgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg ctatgaaatt
      781 gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag
      841 ccatgcggcc tgggcgcgcg tgacacgctg cgtctggaag cgggtatgaa tctttatggt
      901 caggagatgg acgaaaccat ttctccgtta gccgccaaca tgggctggac tatcgcctgg
      961 gaaccggcag atcgtgactt tattggtcgt gaagctctgg aagcgcagcg tgaacatggt
     1021 acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg
     1081 gtacgtttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag cgggactttc
     1141 tccccgacgc tgggttacag cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg
     1201 gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacgaaacc tgtttttgtg
     1261 cgtaacggca aagccgtcgc gtgatttact tttttggaga ttgattgatg agcaacgtac
     1321 cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc gacggcactt
     1381 acaccgttgg catcaccgaa cacgcccagg agctgttagg cgatatggtg tttgttgacc
     1441 tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa
     1501 aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac
     1561 tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa
     1621 tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca tacgaagcat
     1681 tgttagaaga cgagtaacgg ctttattcct cttctgcggg agaggatcag ggtgaggaac
     1741 ttttacgcct caccctcact ctcttcgcaa ggagagaggt tcacaattca ctgcacgttt
     1801 caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg cgcttttatt
     1861 gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca
     1921 caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcagct tgcgaccccg
     1981 ccgcaggttg gcgcaccggc gaccgaatac gccgcgctgg cggaactcaa ggctattgcc
     2041 agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt gcagctaccg
     2101 ccggttattc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta tactccgtat
     2161 caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca ggtaacgctg
     2221 gatttaaccg ggctggatat ggcctctgct tctcttctgg acgaggccac cgctgccgcc
     2281 gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg cttctttgtt
     2341 gcttctgacg ttcatccgca aacgctggat gtggttcgca cccgtgccga aacctttggt
     2401 tttgaagtga ttgtcgacga tgcgcaaaaa gtgctcgatc accaggacgt cttcggcgtg
     2461 ctgttacagc aggtgggtac gacgggtgaa atccacgact acactgcgct tatcagcgaa
     2521 ctgaaatcac gcaaaattgt ggtcagcgtc gccgccgata ttatggcgct ggtgctgtta
     2581 actgcgccgg gtaaacaggg cgcggatatt gtctttggtt cggcgcaacg ctttggcgta
     2641 ccgatgggct acggtggccc acatgcggca ttctttgcgg cgaaagatga atacaaacgc
     2701 tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac cgctctgcgc
     2761 atggcgatgc agactcgcga gcaacatatt cgccgtgaga aagcgaactc caacatttgt
     2821 acttcccagg tactgctggc aaacatcgcc agcctgtatg ccgtttatca cggcccggtt
     2881 ggcctgaaac gtatcgctaa ccgcatccac cgtctgaccg atatcctggc ggcgggcctg
     2941 caacaaaaag gtctgaagct gcgccatgcg cactatttcg atactttgtg tgtggaagtg
     3001 gctgacaaag cgggcgtgct ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat
     3061 attctgaacg cggttgggat cacccttgat gaaaccacca cgcgtgaaaa cgtgatgcag
     3121 cttttcagcg tgctgttggg cgataaccac gggctggaca tcgacacgct ggacaaagac
     3181 gtggctcacg acagtcgctc tattcagcct gcgatgctgc gcgacgacga aatcctcacc
     3241 catccggtgt ttaatcgcta ccacagcgaa accgaaatga tgcgctatat gcactcgctg
     3301 gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg caccatgaaa
     3361 ctgaacgccg ccgccgagat gatccccatc acctggtcgg aatttgccga actgcacccg
     3421 ttctgcccgc cggaacaggc agaaggttat cagcagatga ttgcgcagtt ggctgactgg
     3481 ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc
     3541 gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga agggcatcgc
     3601 gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc acatatggca
     3661 ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct ggctgatctg
     3721 cgcgcgaaag cggaacaagc gggcgataac ctctcctgta tcatggtgac ctatccttcc
     3781 acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc
     3841 ggtcaggttt accttgatgg cgcgaacatg aacgcccagg tcggcatcac ctcaccgggc
     3901 tttattggcg cggacgtctc gcaccttaac ctgcataaaa ctttctgcat tccgcacggt
     3961 ggcggcggcc caggtatggg accgatcggt gtgaaagcgc atttggcacc gtttgtaccg
     4021 ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcggt ttctgcggca
     4081 ccgttcggta gtgcctctat cctgccaatc agctggatgt acatccgcat gatgggcgca
     4141 gaagggctga aaaaagcaag ccaggtggca attcttaacg ccaactatat tgccagccgc
     4201 ctgcaggatg ctttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt
     4261 attcttgata ttcgtccgct gaaagaagaa accggcatca gcgaactgga tattgccaag
     4321 cgtctgatcg actacggttt ccacgcgccg accatgtcat tcccggtggc gggtacgctg
     4381 atggttgaac cgaccgaatc cgaaagcaaa gtggaactgg atcgctttat cgacgcgatg
     4441 ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg tctggccgct ggaagataac
     4501 ccgctggtga acgcgccgca cattcagagc gaactggtcg ccgagtgggc gcatccgtac
     4561 agccgtgaag ttgcggtatt cccggcaggc gtggcagaca aatactggcc gacagtgaaa
     4621 cgtctggatg atgtttacgg cgaccgtaat ctgttctgct cctgcgtacc gattagcgaa
     4681 taccagtaat tcactgattc gactattttc taaaggcgct tcggcgcctt tttagcttgt
     4741 taataaagtg cgctttgtgt gtttcgactg aggcgtaata taaaatattg cgagctcaat
     4801 atattaaaag agtgccaggc ctgatgagcg tagcgcatca ggcacatcgg ctaattacag
     4861 attctcgccg ttgctggcaa tcacctcttt gtaccagtca aagctcttct tacgtgaacg
     4921 cgacatatcg ccagtaccgt cgtcatgttt attcacatag ataaagccgt agcgcttgct
     4981 gtactgcccg gtggtgaacg acacgcagtc gatgcagccc catggtgtgt agcccatcag
     5041 gtcgacacca tcgtaagtca ccgctttttt catctcttca atatgggcgc gcaggtagtc
     5101 aatgcggtag tcgtcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc
     5161 gttttcgaca ataaacagcg gcttctggta acgctcatac agttcgcaaa gtgcgtaacg
     5221 cagacccacc ggatcaatct gccagcccca gtcagatgct ttaacatacg ggtttggtac
     5281 gctgccttca aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ccgcgttggt
     5341 catgtaatag ctgaaaccga gataatcgca ggtgccttcg cgcagcacat cgagatcgcc
     5401 gtcttccatt ttgatgttaa atccgcgacg ctcccactcg tgcaacacat aggacgggta
     5461 atagccacgc agttgcacat cggtaaagac gtagcgttcg cgcatcgact cctgggcgaa
     5521 catcacatcg tccggattac aggagtaagg gtaaagcggc accatcgcca gcatacagcc
     5581 gactttcatt tccgggttaa tgcgacgcgc ggctttcacc gccagggcgc tggcgacaaa
     5641 ctggtgatgc agcacctggt acatcgtctc ttccgggttt tcatgctcgg tatacaccac
     5701 gccggagcag cagtaaccga acagcggtgc acgccagtta cgctggttgt taatttcgtt
     5761 gaaggtcatc cagtatttga ctttatgctt atagcgttca aataccactt ctgcgaaacg
     5821 gacaaagaaa tcaaccactt tacggttggt ccagctaccg tattgctgca ccagatgcag
     5881 cggcatttca aagtgggaga gggtgatcac cggttcgatg ttgtatttca gcagttcatc
     5941 gaacatatca tcgtagaact tcagcccttc ttcgtttggc tgagcttcat cgccttttgg
     6001 aaaaatgcgc gtccaggcga tggatgtacg aaaacatttg aagcccattt cggcaaatag
     6061 cttgatgtct tccttgtaat gaccataaaa atcaacggct tcatggttcg gatagtattt
     6121 tcctggcaac acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac
     6181 gtcacaaatg ctcggccctt tgccgccttt gttccagccg ccttcgacct gatgagcggc
     6241 aactgcgccg ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc
     6301 cagatgagtc aattcttaca ggaaatagta ccatgcagcc aaacgacatc actttttttc
     6361 aacgtttcca ggatgacatt ctggctgggc gtaaaaccat caccatccgc gacgagtctg
     6421 aatcgcactt caaaacgggt gatgtgcttc gtgtcgggcg ttttgaagat gacggttatt
     6481 tttgcacgat tgaagtcacc gcaacctcaa ccgtaacgct ggatacactg acggaaaaac
     6541 atgcagagca ggaaaatatg accctgactg aactcaaaaa ggtcattgcc gacatctatc
     6601 ccggtcagac acagttttat gtgattgaat ttaaatgtct ttaaattgaa ctcacacctg
     6661 ttagttgaaa ttttgcattt aatttcatga tttttcggag attagtccca aatcagaatt
     6721 tattttagct aacaggtgtt cactggaact attctcagtt acgctggaga ggtttacacg
     6781 tgcgtgtacg tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat
     6841 tcgctggcag aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc
     6901 gttgggctgg tgttgctact ggttcaggcg gtggatctca atgccagcgc cacagcgata
     6961 accagttaca gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat
     7021 cacgccattc ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ctgcgctatt
     7081 tatctgttga ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta
     7141 gcgcgcgggt tgatgattgt tatctggagc ctggctctgc tggggattct gttcaaactg
     7201 actatcgcgc accgattcaa aattttatct ctggtgacct atctggcgat gggctggttg
     7261 tcgctggtgg tgatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg
     7321 gctgtaggcg gcgtggtcta ctcgctcggg gtgattttct acgtctgcaa gcgcatccca
     7381 tacaaccatg ccatctggca cggctttgtg ctcggcggca gcgtgtgcca ctttctggcg
     7441 atctatttat atattgggca ggcgtaatgg gtgtgccttt gtaggcctga aaagacacac
     7501 ccgtgtcgca tcaggcgata aatacctgat gcgacacaga gatttactct tcgagcgaat
     7561 acggcagcgg ctcgatatgc agcgtattcg catcgtcacg cacgcggaag atgctgtccg
     7621 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
     7681 cagccagcac cgtaccagtg cgacgccagt tctcgcccat tttcagctct aaatcttcac
     7741 cagcttccgg cagtcggctg gcactacctg tcagcaacca gagcgcgcgt ttgttggcac
     7801 cacggaattt tgctcgcgcc accatctctt gtccggtata gcagcctttt ttaaagctga
     7861 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcgt
     7921 caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
     7981 ccgcttcacc gcgcagttta tcggtcagca tattggcagt agcttcatcg gttacgatca
     8041 ggaaacgttc tgccgggtgt tcgaaccata gcaaagtggt cgcgccttct ttaactacct
     8101 gtttttcttt cgaaggcagt tcactaaaga gatttgccag cgcggcgcgc gcctgaaaac
     8161 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaacaccg
     8221 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccatgcaa
     8281 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
     8341 gggcggcgag caggtgctga tcttctgtca tctggctgac atctgctgtc acctgaccct
     8401 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtta
     8461 tcagcgtcag cggtaaacgg gcagacgccg taggctgacg gggaggaaaa ggtgtaaaag
     8521 ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg
     8581 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa
     8641 atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gattgtgttt actgatattc
     8701 ccgcaaaaga agtcgaaaaa cacgttacaa tagccaagta ctggattttc gtgagacagg
     8761 aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc
     8821 gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac
     8881 gaaaaacgca tctttattcg tctgctggag tgtgacgatc cggacctgtt taactggctg
     8941 atgaatcacg gtaaaccagc cgatgcagaa ctggaaatga tggtccgact catccagaca
     9001 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcgcagtg
     9061 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccgct
     9121 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag
     9181 ccagcggcgc attaatgctc gccaggggga aattcgcctg ttgatggacg ggcgtttgcg
     9241 ttggcaaggg aaggagtgga gcatcgtcaa agcgccgtgg atgattaaga gtggcatgat
     9301 gctgcgttta cgttctgata gcggtaaacg gcaacattta tggctggcag ccgacagcat
     9361 ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata
     9421 aagatgacgg caaagcatat gcgctaagcc gtcagtgaca tcaggcgtaa tgctctgcca
     9481 tttcgttgag gatttgttca caccagctct gaatacgctc gtcgctaagg tcatactggt
     9541 tagtttcgtc cagcgccagc cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
     9601 tggtaaattc atacccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
     9661 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
     9721 caagcccata cagcgcaaca attttacctt cgaggttcag gtcgtcgagc tgatcccaga
     9781 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
     9841 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
     9901 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
     9961 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgtggct
    10021 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    10081 ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg
    10141 gctggaaaaa aatctggctg aaaatacgtt gaacgcatac cgtcgcgatc tgtcaatgat
    10201 ggtggagtgg ttgcatcacc gcgggtcgac gctggcgacg gcgcaaagtg acgatttgca
    10261 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    10321 gagtgcagtg cgtcgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    10381 cagtgcgcat ctcgcttcac cgaaattgcc acagcgtttg ccaaaagatt taagcgaagc
    10441 gcaggtcgaa cgtttattac aggcaccatt aattgatcag ccactggagc tacgcgataa
    10501 agccatgctt gaagtgttgt atgctacagg gctgcgtgtc tctgaactgg tcgggctgac
    10561 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
    10621 gcgcctggta ccgttaggtg aagaggcggt ttactggctg gagacctatc tggaacatgg
    10681 gcgaccgtgg ttgttgaacg gtgtctctat tgatgtgttg ttccccagcc agcgtgcgca
    10741 gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc tggcgggtat
    10801 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa
    10861 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tctccaccac
    10921 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    10981 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    11041 tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt
    11101 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac
    11161 agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    11221 gccaatgtat gacgttagtg gcacagctcc ggtcaatgtc accaataaga tgctgttaaa
    11281 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt
    11341 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    11401 agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    11461 tagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt
    11521 tgatgatgtg atggccggta aaagcgtcgc accagccagt tgcgacgtgg atattgccga
    11581 tcattacgca cttggcgtcc agcttggcgt tagcggtact ccggcaattg tgctgagcaa
    11641 tggcacactt gttccgggtt accagccgcc gaaagagatg aaagaattcc tcgacgaaca
    11701 ccaaaaaatg actagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    11761 gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac
    11821 gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc
    11881 tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa
    11941 ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag cacggctcta
    12001 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt
    12061 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggctcatgc ccgtggcgcg
    12121 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggggttga acacgctcgt
    12181 tcgttgggca ttccggttat tgttaccgat caccatttgc caggcgacac attacccgca
    12241 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    12301 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc
    12361 tggtttgatg agcgcggcat cgcaattcct aatctggcag aactgctgga tctggtcgcg
    12421 ctggggacag tagcggacgt cgtgccgctg gacgctaata atcgcattct gacctggcag
    12481 gggatgagtc gcatccgtgc cggaaagtgc cgtccgggga ttaaagcgct gctggaagtg
    12541 gccaatcgcg atccacaaaa actcgccgcc agcgatttag gttttgcgct ggggccacgt
    12601 ctcaatgctg ccgggcgact ggacgatatg tccgttggcg tggcgctgtt gctgtgcgac
    12661 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    12721 gagatcgaac agggaatgca agttgaagcc ctgaccttgt gcgagaaact ggagcgcagc
    12781 cgcgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc
    12841 ggtattctgg cttcgcgcat caaagagcgt tttcatcgtc cggttatcgc ctttgcgcca
    12901 gcaggtgatg gtacgctgaa aggttccggt cgctccattc aggggctgca tatgcgtgat
    12961 gcgctggagc gattagacac actctacccc ggcatgatgc tcaagtttgg cggtcatgcg
    13021 atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca acgttttggc
    13081 gaactggtta ctgagtggct ggacccttcg ctattgcaag gcgaagtggt gtcggacggt
    13141 ccgttaagtc cggccgaaat gaccatggaa gtggcgcagc tgttgcgcga tgctggcccg
    13201 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcga
    13261 ctggtgggcg aacgtcattt gaaggt
//
LOCUS       DAYPHN010000137        13264 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000137 DAYPHN010000000
VERSION     DAYPHN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.137"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1721
                     /locus_tag="ACG0BW_003636"
     CDS             <1..1721
                     /locus_tag="ACG0BW_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260558.1"
                     /translation="AGFNEQLKENLSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGG
                     DWYPFSEGRRFVSFHHVDELRICASCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQ
                     EIYERPYNSFISDATANGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRL
                     KNARILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQL
                     EVPRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIESV
                     TYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTT
                     QQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTGAGNISALNKVMKGTLVTSLALVAV
                     TTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVV
                     GGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLA
                     RVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAL
                     PPELPA"
     gene            1797..2252
                     /locus_tag="ACG0BW_003637"
     CDS             1797..2252
                     /locus_tag="ACG0BW_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFK7260559.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            2331..2564
                     /locus_tag="ACG0BW_003638"
     CDS             2331..2564
                     /locus_tag="ACG0BW_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFK7260560.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            2664..3482
                     /locus_tag="ACG0BW_003639"
     CDS             2664..3482
                     /locus_tag="ACG0BW_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFK7260561.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            3564..4043
                     /locus_tag="ACG0BW_003640"
     CDS             3564..4043
                     /locus_tag="ACG0BW_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFK7260562.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            4059..4535
                     /gene="yeeS"
                     /locus_tag="ACG0BW_003641"
     CDS             4059..4535
                     /gene="yeeS"
                     /locus_tag="ACG0BW_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFK7260563.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            4598..4819
                     /gene="yeeT"
                     /locus_tag="ACG0BW_003642"
     CDS             4598..4819
                     /gene="yeeT"
                     /locus_tag="ACG0BW_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFK7260564.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4893..5261
                     /gene="cbeA"
                     /locus_tag="ACG0BW_003643"
     CDS             4893..5261
                     /gene="cbeA"
                     /locus_tag="ACG0BW_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFK7260565.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            5350..5724
                     /gene="cbtA"
                     /locus_tag="ACG0BW_003644"
     CDS             5350..5724
                     /gene="cbtA"
                     /locus_tag="ACG0BW_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFK7260566.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            5721..5915
                     /gene="yeeW"
                     /locus_tag="ACG0BW_003645"
     CDS             5721..5915
                     /gene="yeeW"
                     /locus_tag="ACG0BW_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFK7260567.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            5961..6041
                     /locus_tag="ACG0BW_003646"
                     /pseudo
     CDS             5961..6041
                     /locus_tag="ACG0BW_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6330..6458)
                     /locus_tag="ACG0BW_003647"
                     /pseudo
     CDS             complement(6330..6458)
                     /locus_tag="ACG0BW_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6530..6712
                     /locus_tag="ACG0BW_003648"
     CDS             6530..6712
                     /locus_tag="ACG0BW_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFK7260568.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(6813..7142)
                     /gene="tmaR"
                     /locus_tag="ACG0BW_003649"
     CDS             complement(6813..7142)
                     /gene="tmaR"
                     /locus_tag="ACG0BW_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFK7260569.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7314..8372)
                     /gene="yeeA"
                     /locus_tag="ACG0BW_003650"
     CDS             complement(7314..8372)
                     /gene="yeeA"
                     /locus_tag="ACG0BW_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFK7260570.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(8570..9043)
                     /gene="sbmC"
                     /locus_tag="ACG0BW_003651"
     CDS             complement(8570..9043)
                     /gene="sbmC"
                     /locus_tag="ACG0BW_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFK7260571.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(9162..10328)
                     /gene="dacD"
                     /locus_tag="ACG0BW_003652"
     CDS             complement(9162..10328)
                     /gene="dacD"
                     /locus_tag="ACG0BW_003652"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFK7260572.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            10537..11964
                     /gene="sbcB"
                     /locus_tag="ACG0BW_003653"
     CDS             10537..11964
                     /gene="sbcB"
                     /locus_tag="ACG0BW_003653"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFK7260573.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPELLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(12007..12234)
                     /gene="tsuB"
                     /locus_tag="ACG0BW_003654"
     CDS             complement(12007..12234)
                     /gene="tsuB"
                     /locus_tag="ACG0BW_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFK7260574.1"
                     /translation="MAIKKLDVVTRVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(12248..>13264)
                     /gene="tsuA"
                     /locus_tag="ACG0BW_003655"
     CDS             complement(12248..>13264)
                     /gene="tsuA"
                     /locus_tag="ACG0BW_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFK7260575.1"
                     /translation="LGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVFA
                     LIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTY
                     MVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVLLVITLWVVMKELKK
                     PKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSP
                     TANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGV
                     LMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLAT
                     AAAN"
BASE COUNT         3406 a         3461 c         3254 g         3143 t
ORIGIN      
        1 tggcagggtt taacgaacag ttgaaagaga acctgtcatt cacacctgat gaactgaaaa
       61 cgctcttccg gaaccgcctg aatttatcac cggaagcgaa tgacgatttt ctcgatcgtt
      121 gcctggacgg cggtgactgg tatccctttt cagaaggtcg ccgttttgta tcattccatc
      181 acgtggatga gcttcgtatc tgtgcctcct gcgggctgac agaagtacat catgccccgg
      241 aaaatcataa gccggatccg gaatggtact gctcctctct ttgccgcgaa acagaaacac
      301 tgtgtcagga aatttatgaa cgcccttaca acagctttat ttccgatgca acggcgaatg
      361 gtctgattct catgaaactg ccggaaacct ggagtacaaa tgagaaaatg tttgcttccg
      421 gagggcaggg acatgggttt gccgctgaac ggggaaacca tattgtcgac agagtccgtc
      481 tgaaaaacgc acggatcctc ggtgataata atgccagaaa tggagcagac agactggtca
      541 gcggaacaga aatccagacg aaatattgtt caactgcagc ccgtagcgtc ggtgcggcat
      601 tcgacggaca aaacggacag tatcgttaca tgggaaataa tggccccatg caactggaag
      661 tcccccgtga tcagtatgcc ggcgctgttg aaaccatgag gaataagatc cgcgaaggta
      721 aagtacccgg tgtaaccgat cccgcagaag cgtcccggct gattcgtcgg ggacatctga
      781 cttatactca ggcccgtaat atcacccggt tcgggaccat cgaatcggtc acttatgata
      841 ttgccgaggg ttcggttgtc agtctggcgg ccggagggat cagttttgcc ctgacggcat
      901 cggtcttctg gctcagcacc ggcgatcgcg atgctgccct gcagacagct gctgtccagg
      961 caggaaaaac cttcacccgc acactggctg tctacgtcac aacccagcaa ctccaccggc
     1021 tcagtgttgt tcagggtatg ctgaagcaca ttgatttttc gactgccagc ccgactgtcc
     1081 gactggctct tcagaagggg accggtgcag gaaatatcag tgccctgaac aaagtaatga
     1141 agggtacgct ggtgacatct ctggcactgg tagctgtcac aaccggccct gacatgatca
     1201 aaatgttgcg gggacggatc tccggtgcgc agttcatcag gaatcttgcc gtggcatctt
     1261 ccggtgtggc aggtggagct gtcgggtcag tggcgggcgg gatactgttc agtccactgg
     1321 gaccatttgg tgcactgaca gggcgtgtgg ttggcggtgt tctgggggga atgattgcct
     1381 ccgctgtatc aggaaaaatt gccggagcgc tggttgaaga agatcgcgtc aaaattctgg
     1441 caatgattca ggagcaggtg acatggcttg ccggcagttt cctgctgacc ggacatgaga
     1501 ttgaaaatct gaacgagaat ctggcccgtg ttatcgatca gaatgctctg gagatcattt
     1561 tcgccgccgg tatacaacaa cgggccgcga ccaatatgtt aatcaaacca ctggtggtca
     1621 gtatcatcag gcaacgcccc gtcatggaat atgatgcatc ccatctcggc aatatggtta
     1681 accgactgga agaagcatta cccccggagt taccggcata acggctattc tgcgcctttt
     1741 acgctttatc acctgatttg tcagtgtcat cgtcatgact cagagaggta aataccatga
     1801 ttcacctgtt caaaacctgc atgattaccg ccttcattct ggggttaacg tggtctgccc
     1861 cactccgggc acaggatcaa cgttacatca gtatacgcaa tacagatacg atatggctcc
     1921 cgggaaatat ttgtgcttac cagttccggc tggataatgg cggaaacgat gaaggatttg
     1981 gccccctcac catcactctg caactcaaag acaaatatgg tcagacgctg gtgaccagaa
     2041 aaatggaaac ggaagctttt ggtgacagta atgccacgcg aaccacagac gcatttctgg
     2101 aaacggagtg cgtggaaaat gtcgccacaa ccgaaatcat taaagcaact gaagaaagta
     2161 acggccatcg tgtcagtctg ccgttatcgg ttttcaatcc ccaggactac catccactgc
     2221 tgattaccgt ttccggaaaa aacgttaact gatccctgaa gtcagtttca tgcttcagcc
     2281 tctgtcacaa cgcgtggcag aggcttttta tttatcagga gaaaacagat atgccgggtt
     2341 acacagagta tgtcctggct gaaggatctt tctcatacgg gcaggcggtt gctgtcataa
     2401 ccgcttaccg caatgtgttt atccaggatg acccgggaat gcatttccgt cgggttatcc
     2461 gcaacgcgga aggtcagcga cgctggcgct gccggaattc tgaacccgat gccggaaagg
     2521 tgcttaatac gcggctcgcc agtgacggtc ttctcagaca ataaacgtca ttcatttcac
     2581 ccatcaggcc gcgtcttctc cgggagacgt ggctttttca tttattacac cagtaaatct
     2641 taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat gcgaaccaga
     2701 tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgtgccg gggattttcg
     2761 gggaagataa acacacgtcc cgaagccaga actatacgta catccccacc atcaccgtac
     2821 tggaaagcct gcagcgggaa ggctttcagc cattcttcgc ctgccagacc cgtgtgcgcg
     2881 acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa
     2941 acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc
     3001 agatgctgcc ggggtacttc aggttcgtct gccagaacgg ctgcgtctgt ggtcagtctc
     3061 tgggggaagt acgtgttccg caccggggaa atgtggtgga cagagtcatt gaaggggctt
     3121 acgaggtggt gggcgtgttt gaccggatag aggagaagcg tgatgccatg cagtcgctgg
     3181 tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg
     3241 acgaacatca gcccgtcacc accgccgaca ttctgacgcc gcgacgccgg gaggattacg
     3301 gtaaggacct gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt
     3361 ccggtcgcag tgcaaaagga aaacgtatcc atacccgggc cattcacagc attgataccg
     3421 acattaagct caatcgcgca ttgtgggtga tggcagaaac gatgctggag agcctgcgct
     3481 gatgccgttt ccctgagtga gggaatcatt cagctttcgt tcctgaaaaa cacacattta
     3541 tttgttattt aaggagttat ctcatgaaaa ccgtttctca gaataccccc acaatttatt
     3601 cagctacaac accagagaat aatccgcctc agttggttgc cagcctcgtc cctgatgaac
     3661 agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg accctggagc
     3721 cccgtgtctt cggctggatg gaccgtctgt gcgaagacta ctgcggtggt atctggaatc
     3781 tgtacaccct gaacaacggc ggggcattta tggcacccga accggatgac gatgatgacg
     3841 aaacatgggt actgttcagt gccatgaacg gtaaccgcgc agaaatgagc ccggaagccg
     3901 ccggtattgc cgcctgtctg atgacgtaca gtcatcatgc ctgtcgtacg gagaattatg
     3961 ccatgacggt ccattattac cggttgcggg attacgccct gcagcatccg gaatgcagcg
     4021 ccattatgcg catcatcgac tgaatggaga gaagcacaat gcaacaactt tcctttctgc
     4081 ccggagagat gacgcccggc gagcgcagcc tcattctgcg ggctctgaaa accctggacc
     4141 gccatcttca tgaacccggc gtggccttca cctccacccg tgcagcacgg gaatggctga
     4201 ttctgaacat ggcgggactg gagcgtgaag aattccgggt gctgtatctg aacaaccaga
     4261 atcagctgat tgccggtgaa accctcttca ccggcaccat caaccgcacg gaagtccatc
     4321 cccgggaagt gattaaacgc gccctgtacc acaatgccgc tgccgtggta ctggcacaca
     4381 atcacccgtc cggtgaagtc acacccagta aggcagaccg gcttatcacc gaacgtctgg
     4441 tacaggcact gggcctggtg gatatccggg tgccggacca tctgatagtc ggtggcaacc
     4501 aggttttctc ctttgccgaa catggtctgc tttaaccctt cacaaccaca tcacacctgt
     4561 tttcactttt atcttctgtc ttcagaggta tcccattatg aaaattatca cccgtggtga
     4621 agccatgcgt attcaccaac aacatccgac atcccgtctt tttccgttct gtaccggtaa
     4681 ataccgctgg cacggcagtg ctgaagcgta taccggtcgt gaagtgcagg atattcccgg
     4741 tgtgctggcc gtgtttgctg aacgccgtaa ggacagtttt ggtccgtatg tccggctgat
     4801 gagcgtcacc ctgaactgag tgggaattct gatgagcaga attatcgcca ctaccggacc
     4861 attcttagcc gattttctgt aaggatttta tcgtgtcaga cacactcccc gggacaacac
     4921 ttcccgacga caatcacgac cgcccctggt gggggctgcc ctgcaccgtg acgccctgtt
     4981 tcggggcacg tctggtgcag gagggtaacc ggttgcatta ccttgccgac cgcgccggta
     5041 tcagaggcct gttcagcgat gcagatgcgt accacctgga ccaggccttt ccgctgctga
     5101 tgaaacaact ggaactcatg ctcaccagcg gtgaactgaa tccccgccat cagcataccg
     5161 tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcagcagt tgtggttacg
     5221 tttatctggc tgtttatccg acgcccgaaa tgaaaaatta actctccaga atagccttct
     5281 gctacggcct ggtgttttca ccacgccact tttccatttt tatatctgca tatcaggaaa
     5341 atcttcagta tgaaaacatt acctgtatta cccgggcagg cggccagttc tcgcccgtct
     5401 cctgttgaaa tctggcagat actgctgtcc cgactgctgg accagcacta tggcctcaca
     5461 ctgaatgaca caccttttgc cgatgaacgt gtgattgagc agcatattga ggcaggcatt
     5521 tcactgtgtg atgcggtgaa ctttctcgtg gaaaaatacg cgctggtgcg taccgaccag
     5581 ccgggattca gcgcctgtac ccgctctcag ttaataaaca gtattgatat cctccgggca
     5641 cgccgggcaa ccggcctgat gacccgcgac aattacagaa cggtaaataa cattaccctg
     5701 ggtaagtatc cggaggcgaa atgatgacac tggaagccga cagcgttaac gtacaggcgc
     5761 tggatatggg gcacattgtc gttgacattg atggtgttaa tatcactgaa ctgattaata
     5821 aggccgctga aaacggttat tcactccgcg tggtggatga ccgtgactct accgaaacac
     5881 cggcaactta tgccagccct caccagttgc tgtaagacaa tgcagtgata cagggtatag
     5941 tgccattaac cacagaaaca gcactgttgt cctgtattaa gcaggcaaga gaagatgtcc
     6001 ttaccctgcg ccatctgcag cttctgcacc agaaccggtg aatctcactc aacaggcttc
     6061 atttctcctg acgccagcct gaatacagct ggcgttttca tttataaaca gaaaggaaaa
     6121 ccgataataa tggaatggac gccccgacca tgaagcaacg gtaggatatt actgataaac
     6181 cttccagtgg aggtgcgtta tggccgtttt aacgattagc attgacctga caaaaaatga
     6241 attccaaatc cacggtctgg gtaggaacag aaaaatctaa gctcagaaaa cggattaaag
     6301 taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagaactac gttccatggc
     6361 aggagttcgt caacacggtt ggagggccat tccggcagta tgctcagaat atggcgcaga
     6421 tacgcttccg gatcgatacc gttcagacgg caggtgcctg cttttctggc aacaagcagg
     6481 aatctcagcg ctgaagcagg aattagaggt aaaaacgccc taccgggcca tgaatcaccc
     6541 ggtcattgga gtagtaacca aagcagattt agccagcatg gaacaaatat ctttggtgaa
     6601 aagttggtta cgggaagctg gagcgcacaa cgtgttagta accagtgcag ttaataacaa
     6661 tggagttaca gaactttttg ccttgctgca tacagaagaa ggctgttgtt aatttcgatt
     6721 ggttctcgga tgagaatgat gacggtgata cacagataag aatcaatgag caatgagggt
     6781 tgccgggcaa ccctcattga ataaaacggg aattacttcg cttcgccgtt tttcatttcg
     6841 cccatagctt tcagcttttt ggagatatcg cggcgttctt tggagagctc ggcatttttg
     6901 atgatgtaat cgtcaacgcg atcttcatag tcacctttca tgctggcgat gatgccctgg
     6961 attgcttcaa cgctcatccc cggcttgatg taatcgctca ggttgtccag cagcaggacg
     7021 cgcttctggt tgtcacggat ctttttctca acgtcctgaa tttcacgttg cagtttgttc
     7081 ttacgacgga acagacgaac aaattccagt acgtcctgga atgaaggctt ggtagtttcc
     7141 atttttatac ccctgataat gtgagagtcg gattcgttta agcaaccgct attcgttagg
     7201 gccaacatta ctcatagctg ccgcagatga caatgctttt atcccttttc actatcatac
     7261 cctttatcct tgctgaatcg aagcagcagc aagatgattc tgaagttcag gaattatttg
     7321 cgcaaggccc ggcaaatcag attcgatagc aactcaagct gatgcgccgt ttccatgttc
     7381 agccacacat aaccatagat tggtgtttcc acaaccttca ggtcatggtg gttattgagc
     7441 aactgacgca gctcttccac agcatcgttc aatttttccg tattggcaaa aaccggctgc
     7501 ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat catgtgctgg
     7561 gtatcacgaa gtttttgcgc gttcaataac acgaaatggc tggggcgcgt ggcccagtat
     7621 gcattgattt gcaactccag catacaaacc agattgcggt taatggtctg gataccttca
     7681 tatatcgatt ttggaatacg ggtttctttg ctggcgggcg caatcagtcc acgcattttc
     7741 acggcatcgg tcaggagttt ttgtagatgg ctttccagac gtgggcgttc gagtaagttc
     7801 ggtgagaatg cagattgata gacccgatta tactcggtca gacttttcgc cagttgaatg
     7861 cgccagtgga tgaacgcccg ttgtggccag ataccggtaa acaacattgc cagtaaagag
     7921 ccgaggatca catcgccgct tcgccataac gccgtatcaa tttcacctgt cggggaaccc
     7981 acaacaattg ccagcgtcac cccaatcaat aaaccttgat acggtttctt gcccagcgcc
     8041 agccaaccgc aaaggaacat ggccgccgcg caccagacta acatcagcgg taacgagatt
     8101 aactccagtt gcagagcgat aagacctaaa atcgaaccca acaccgtacc gccaatacgc
     8161 tcaaaggcgc gagggacaac gttaccccag aacgagattg gccccataat caccaccatg
     8221 gtgaccagcg gccaggtgct ttccgggata gtaaacaggc ggataatgag aaaagtgagc
     8281 aggaatgcca gcgcgacccg agtaccatgc acaatgcggt aatggcggta tacccggatt
     8341 tcaaacgggc ttaatgactt atcggcacgc acgcagaaac tccgtaatga gattaaaagc
     8401 gctcataact catctgtcgg ccagaaggcg cgcctctgaa ttatccgacg gacaaaccgg
     8461 gaacagtgta atttcccggc aggagcgaaa aaattgaaag gcgcatctta ctcttttcgc
     8521 tttcaaaaaa aagagtggtc atcgcgttaa cacaccgccc tgagatgaat tagtgatgtt
     8581 ttggctgcac cgcaacatac atttcgatat cccagtaccc atcttccgcg ccattgttca
     8641 aataaacctc gaagcagggc tttggtaaca tttcataagc actgtcctgc aagaggctat
     8701 taaagaactg ataccagggt ttagcaaaat catcaccgac tacacgagct accgccaccg
     8761 catactgacc acctgaaatt tctgtcagaa tgacgccctc actgttttcg gggagcgtaa
     8821 agtaacccgg caccgtcacg acggtgtcgc agcgtaattt ttcggcgggt gtttcatctg
     8881 gattgtcgta atagacagca acccattcct tcggcacaat atttttgcta tctacccaca
     8941 tcatcaactg ctcaaaacct ttctttaccg tctgttccca cgggccaacg agatggaaac
     9001 ctgcaacggt acgtttatct tcctgcttaa tctcgtagtt catgacgcct ccattaatac
     9061 tgtttttata tacagtatag ttgcaatatt aaaaccacaa ggaatgagtg ttgattatgc
     9121 gagcagactc gcactcctgc cagtctgctg caaaagaaag gtcaggcctt atggtggaaa
     9181 taatcactaa ggcgagaaaa catgctgcct tccccgacag attccagggt aaccagcggc
     9241 cagtgcgcca cctgtttatc acggtcgtaa agttcaattt cccctacccg ctgatgggcg
     9301 ctaattggcg cggtgagttc tttaccatca agggtatatt tggctttgat atgtggaatt
     9361 tcggctttcg gtagcaccat ccagaactct tgttccgttc ccaggtcgat attttcttta
     9421 tcgccatacc agatgcgttc cgttccgacc tttttcccac ggtgcaaaat ttgcaccgta
     9481 gtaaagtttt gttgccccca acgcagtaat tttcttgcct cttcctcacg accttttgca
     9541 ctgtcagccc ccattatcac tgcaatgaga cgatgctgcc catctacagc cgaagcaatg
     9601 agattaaacc cggcaccaga agtatgaccc gttttcaggc cgtcaacatt catggtttta
     9661 tcccacaata atccgttacg gttttgctgg gtgataccgt tccaggtgag acttttctca
     9721 ctgtacatat gataaaactc gggctcgccg tggatgatag cgcgagaaag cacagctaaa
     9781 tcataagccg agctatgctg gccaggtgca tccagaccat gcactgtttc aaaatgcgta
     9841 tccttgagat gcagcttctc ggcatagttg ttcatcattt caacaaactg ccgttgccca
     9901 ccggcaatat agtcagccag tgcaacacaa gcgtcatttc cggaatccac aattaaacca
     9961 cggcttaaat cacgtaccga tacgcgatcg ccctctttca aaaacatcag tgaagaaccg
    10021 acaaacaccg gattatcttt cgcccacgca tcacgcccca cggtgacaat atcgtctggc
    10081 gtaatgcgat gactatcgat agcgcgatcc acgacatagc ccgtcatcag ctttgtcagg
    10141 ctggcgggat tgcgctgttg atgctcatta cccgcggtga gaatctgacc ggtggtgtaa
    10201 tccatcaata cccaggaccc ggcatgaatc tctggaggtt gaggtgaaaa aggaatgttt
    10261 tccgccgcaa aaccagacga taaattaaaa acgaacaaag aagcagcaat aataagacgg
    10321 cgtttcaaca gcaacccctc aggagtttca aatagctgtt ctttttacgg aaatacttat
    10381 gaactggctg gaataaagtg caagaaaatg tgactaccct ctcattttta tctgacatga
    10441 tctgttgcca ctcgctgcca aattgtcgcg ctaaagctga ttagcacggt gatatttgat
    10501 actctggcag acagcagaaa caacggattt aacctaatga tgaatgacgg taagcaacaa
    10561 tctacctttt tgtttcacga ttacgaaacc tttggcacgc accccgcgtt agatcgccct
    10621 gcacagttcg cagccattcg caccgatagc gaattcaatg tcatcggcga acccgaagtc
    10681 ttttactgca agcccgcgga tgactattta ccccagcctg gagcagtatt aattaccggt
    10741 attaccccgc aggaagctcg ggcgaaagga gaaaacgaag ccgcatttgc cgcccgtatt
    10801 cactcgcttt ttaccgtacc gaagacctgt attctgggct acaacaatgt gcgtttcgac
    10861 gacgaagtca cacgcaacgt tttttatcgt aatttctacg atccttacgc ctggagctgg
    10921 cagcatgata actcgcgctg ggatttactg gatgttatgc gtgcctgtta tgccctacgc
    10981 ccggaaggaa taaactggcc tgaaaatgat gacggtctac cgagctttcg ccttgagcat
    11041 ttaaccaaag cgaatggtat tgaacatagc aacgcccacg atgcgatggc tgatgtgtac
    11101 gccactattg cgatggcgaa actggtaaaa acgcgtcagc cacgcctgtt tgattatctc
    11161 tttacccatc gtaataaaca caaactgatg gcgttgattg atgttccgca gatgaaaccc
    11221 ctggttcacg tttccggaat gtttggggca tggagcggca ataccagctg ggtggcaccg
    11281 ctggcgtggc atcctgaaaa tcgcaatgcc gtaattatgg tggatttggc aggagacatt
    11341 tcgccattac tggagctgga tagcgacaca ttgcgcgagc gtttatatac cgcaaaagca
    11401 gatcttggcg ataacgccgc cgttccggtt aagctggtgc atatcaataa atgtccggta
    11461 ctggcccagg cgaatacgct acgcccggaa gatgccgacc gactgggaat taatcgtcag
    11521 cattgcctcg ataacctaaa aattctgcgt gaaaatccgc aagtgcgtga aaaagtggtg
    11581 gcgatattcg cggaagccga accgtttacg ccttcagata acgtggatgc acagctttat
    11641 aatggctttt tcagtgacgc cgatcgtgca gcaatgaaaa ttgtgctgga aaccgagccg
    11701 cgtaatttac cggcactgga tatcactttt gttgataaac gaattgaaaa gctgttgttc
    11761 aattatcggg cgcgcaactt cccggggacg ctggattatg ccgagcagca acgctggctg
    11821 gagcaccgcc gccaggtctt cacgccagag ttattgcagg gttatgcaga tgaattgcag
    11881 atgctggcac aacaatatgc cgatgacaaa gagaaagtgg cgctgttaaa agcgctttgg
    11941 cagtacgcgg aagagatcgt ctaatttgaa gcctccgccg ctggtacggc ggaggacaat
    12001 gttgagtcag gctttttgaa cggtgatgct ccacgctgca tcgccaatct gctgataatc
    12061 ggtgatggca tgtccttctt ctgccgccca ctgtgggatt gcttcagttg cctgggtgca
    12121 atcaaactca attaccaact catcgccgct ggccatttct gccagtgctg cttttgcttc
    12181 tatgagtgga aacggacaga cccgcgtcac cacatccagc tttttaatcg ccatctttac
    12241 accttactta atttgccgca gcagttgcca gtcgcgcctt acgctgcggt cgaacataca
    12301 caagccagga cgcagtccag actccgagaa tcataaatac aaggccaatc cagccctgcc
    12361 aggtcatcat tgcagtcatg accagtccat taccgataga gcaaccacct gcaatgctgg
    12421 caccgaagcc catcagtaca ccacctaacc cgctacgtaa tgttgtttgt gcatcagcag
    12481 cgcgaacgcg gaactcacgg ctcgctttgg cggcaataaa tgaccccacg aagatcccta
    12541 acaccaggaa aacgccccag ttaatgtatt tcacgtcacc cgcgaccaga aattgcagaa
    12601 tattggccgt tggggaagtg atgcctaacc caaacatacg tccggttgct tcactcaggg
    12661 gccaggctaa aagcgcaatc aaaccgatga gtacagctgt gacaaaggga tgccagcgtt
    12721 tttcaaacag aatatgagcg atcccggttc ggcgcggcgg taaggtcgcg actttgagtt
    12781 ttggcttctt caactctttc atcaccaccc agagcgttat caccagcaaa acggcaacca
    12841 acggccacac agacaaatta aaagtatcag caatagagtt atgttcagta gtgtagtgct
    12901 gcaaggtttg atttaaacca ctggcgtgtg gagaacgcat caccgcactc atcaccatat
    12961 aagtgaaaag cgcgatccaa ctgccgatca atccctcacc cgcgcgatac caggtcccgg
    13021 tggcacatcc acccgccaga acaattccca gcccgaaaag atacccacct ataacagtac
    13081 caagccacgg gaacgccccg gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa
    13141 cccccacgct ttgtaccgaa attgcaatca gcaaggcgta aaacatgcga ttatttttcg
    13201 cgatatacat atcgcgaaaa ccacctgtca ggcaaaaacg cccacgctgc atgacaaatc
    13261 ccag
//
LOCUS       DAYPHN010000138        13208 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000138 DAYPHN010000000
VERSION     DAYPHN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.138"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            46..588
                     /gene="fimA"
                     /locus_tag="ACG0BW_003656"
     CDS             46..588
                     /gene="fimA"
                     /locus_tag="ACG0BW_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFK7260576.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            808..1500
                     /gene="fimC"
                     /locus_tag="ACG0BW_003657"
     CDS             808..1500
                     /gene="fimC"
                     /locus_tag="ACG0BW_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFK7260577.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPYTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1531..4140
                     /gene="sfmD"
                     /locus_tag="ACG0BW_003658"
     CDS             1531..4140
                     /gene="sfmD"
                     /locus_tag="ACG0BW_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFK7260578.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTVNTGDKSGGLMP
                     CFNQALLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4153..5160
                     /gene="fimH"
                     /locus_tag="ACG0BW_003659"
     CDS             4153..5160
                     /gene="fimH"
                     /locus_tag="ACG0BW_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFK7260579.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPNDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5171..5686
                     /gene="sfmF"
                     /locus_tag="ACG0BW_003660"
     CDS             5171..5686
                     /gene="sfmF"
                     /locus_tag="ACG0BW_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFK7260580.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLTAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
     gene            complement(5689..6321)
                     /gene="fimZ"
                     /locus_tag="ACG0BW_003661"
     CDS             complement(5689..6321)
                     /gene="fimZ"
                     /locus_tag="ACG0BW_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFK7260581.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            6564..6640
                     /locus_tag="ACG0BW_003662"
     tRNA            6564..6640
                     /locus_tag="ACG0BW_003662"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6598..6600,aa:Arg,seq:tct)
     gene            complement(6656..7819)
                     /gene="intD"
                     /locus_tag="ACG0BW_003663"
     CDS             complement(6656..7819)
                     /gene="intD"
                     /locus_tag="ACG0BW_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFK7260582.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(7981..8046)
                     /locus_tag="ACG0BW_003664"
                     /pseudo
     CDS             complement(7981..8046)
                     /locus_tag="ACG0BW_003664"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(8046..8351)
                     /gene="yfdT"
                     /locus_tag="ACG0BW_003665"
     CDS             complement(8046..8351)
                     /gene="yfdT"
                     /locus_tag="ACG0BW_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFK7260583.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRVKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMTQQEVK"
     gene            complement(8351..8713)
                     /locus_tag="ACG0BW_003666"
     CDS             complement(8351..8713)
                     /locus_tag="ACG0BW_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260584.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(8704..9240)
                     /gene="yfdR"
                     /locus_tag="ACG0BW_003667"
     CDS             complement(8704..9240)
                     /gene="yfdR"
                     /locus_tag="ACG0BW_003667"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFK7260585.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EEYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            complement(9368..10192)
                     /gene="yfdQ"
                     /locus_tag="ACG0BW_003668"
     CDS             complement(9368..10192)
                     /gene="yfdQ"
                     /locus_tag="ACG0BW_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFK7260586.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQRSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(10258..10620)
                     /gene="yfdP"
                     /locus_tag="ACG0BW_003669"
     CDS             complement(10258..10620)
                     /gene="yfdP"
                     /locus_tag="ACG0BW_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFK7260587.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            11082..11585
                     /locus_tag="ACG0BW_003670"
     CDS             11082..11585
                     /locus_tag="ACG0BW_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260588.1"
                     /translation="MDKFDRSAQRQLLQILYDAHPYEISDDALQSVRDAFGDDNVLIS
                     NLIYLEEHGLIKNALDYYLDGININIPELRITKDGIDFIRDDGGLKAILGIMTIRLHD
                     ETLCELERIINSSTSATTEDKKKLLSQLRTLPADAIKQLTIRLLGQGLDHLPDVFHVI
                     QKALHSL"
     gene            complement(12109..13185)
                     /locus_tag="ACG0BW_003671"
     CDS             complement(12109..13185)
                     /locus_tag="ACG0BW_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFK7260589.1"
                     /translation="MTKSICFFNHKGGVSKTTTTFNLGWALADEGKKVLMVDLDSQCN
                     LTGMVLGYEKIDEGLDSFYSSRDNLTLGPIVEYLINGGQPEAYLEKETGKLHPTLHEN
                     LLLLPGHLDVSDLDSQISVSLKIAAGIPATRNIPGNLPKILQLIAMKNNIDYILYDLS
                     PNVGGLNEVVLMSSDYFIVPVAPDFFCWQAIRSLSKNIPRWHKELALFKENNDGNSSQ
                     SIKNAPQFLGMIQQRYRPRKGAPVKSFEKWMAAIRDAVDSILVPNLEKITCIIPRDKV
                     QHAINKTTVSGDLSAYDLAHISDFNSLIAISQQLATPVFSISDQQIRDSGQFGHALNT
                     MKSSRDAFNEQFKGLAQRVLELTK"
BASE COUNT         3439 a         2903 c         2996 g         3870 t
ORIGIN      
        1 ttatatacgg atgtggagtc gataaatgag attgaaggaa tatatatgaa attaagattt
       61 atttcgtctg cgctggccgc cgcactattc gccgctacgg gtagttatgc tgccgttgta
      121 gatggcggta caattcactt tgaaggcgaa ctggtgaatg ctgcctgttc agtgaatact
      181 gactcggcag accaggttgt cacactcggt caatatcgta ccgatatttt caatgctgtt
      241 ggtaatacct ctgcattaat tccattcact atacagttga acgactgcga tcctgtcgtt
      301 gccgctaatg ctgccgttgc attttctggt caggcggatg caatcaatga taatttattg
      361 gccattgcat ccagtactaa tacaacaaca gcaacgggtg tcggtattga aatacttgat
      421 aatacatccg caattctcaa acctgatggg aatagcttct caaccaacca gaacttgatc
      481 cccgggacca acgttcttca tttttctgca cgttataaag gcaccggtac aagtgcatca
      541 gcagggcaag caaatgctga cgcgactttt attatgagat acgaataatc aaaaccacgt
      601 tgttttgaat tatatatcac gtcttataac aaagtaatgt accggttgtc tgaagcggta
      661 tggtggcaat gtaaatcgaa atcatattca ctttgtatca tgccgcttta ttaaatgaaa
      721 agggaatgat gtgttgtaag aaaccaaagc aatcatttct ttatatttct tatttttgcc
      781 gtcaggaata cacaaggcgt attaactatg atgactaaaa taaagttatt gatgctcatt
      841 atattttatt taatcatttc ggccagcgct catgctgccg gagggatcgc attaggtgcc
      901 acgcgtatta tttatcccgc tgatgctaaa cagactgcgg tatggattag aaatagccat
      961 accaatgagc gctttctggt caattcatgg attgaaaaca gcagcggtgt aaaagaaaag
     1021 tcattcatca ttacaccgcc actgtttgtt agtgaaccca aaagcgaaaa tactttgcgt
     1081 attatttaca ccggtccacc gctggcggca gatcgtgagt ctctgttctg gatgaatgtt
     1141 aagacgatcc cttcggtaga taaaaatgca ttgaacggca ggaatgtttt gcaactggcg
     1201 attttatcgc gcatgaaatt atttctccgt ccaattcaat tacaagaatt acccgcagaa
     1261 gcgccgtaca cactcaagtt ttcgcgatcc ggtaactata tcaatgttca taatccatca
     1321 cctttttatg tcaccctggt taacttgcaa gtgggcagcc aaaaactggg gaatgctatg
     1381 gctgcaccca gagttaattc acaaattccc ttaccctcag gagtgcaggg aaagctgaaa
     1441 tttcagaccg ttaatgatta tggttcagta accccggtca gagaagtgaa cttaaactaa
     1501 ccgaatcatc tgacaatatc agagctaatt atgaaaatac ccactactac ggatattccg
     1561 cagaggtata cctggtgtct ggccggaatt tgttattcat ctcttgccat tttaccctcc
     1621 tttttaagct atgcggaaag ttatttcaac ccggcatttt tattagagaa tggcacatcc
     1681 gttgctgatt tatcgcgctt tgagagaggt aatcatcaac ctgcgggcgt gtatcgggtg
     1741 gatctctggc gtaatgatga gttcattggt tcacaggata tcgtatttga atcgacgaca
     1801 gtaaatacag gtgataaatc aggtgggtta atgccttgtt ttaatcaggc actccttgaa
     1861 cgaattggcc ttaatagcag tgcattcccc gagttagccc agcagcaaaa taataaatgc
     1921 attaatttac tgaaagctgt acctgatgcc acaattaact ttgattttgc agcgatgcgc
     1981 ctgaacatca ctattcctca gatagcgttg ttgagtagcg ctcacggtta cattccgcct
     2041 gaagagtggg atgaaggtat tcctgcttta ctcctgaatt ataatttcac cggtaacaga
     2101 ggtaatggta acgatagcta tttttttagt gagctcagcg ggattaatat tggcccgtgg
     2161 cgtttacgca acaatggttc ctggaactat tttcgcggaa atggatatca ttcagaacag
     2221 tggaataata ttggcacctg ggtacagcgc gccattattc cgctgaaaag tgaactggta
     2281 atgggagacg gcaatacagg aagtgatatt ttcgatggcg ttggatttcg tggtgtacgg
     2341 ctttattctt ccgataatat gtatcctgat agccagcaag ggtttgcccc aacggtacgt
     2401 gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat tatctatcaa
     2461 agctatgttt cccccggcgc ttttgaaatt acagatttgc acccgacatc ttcaaatggc
     2521 gatctggatg tcaccatcga cgagcgcgat ggcaatcagc agaattatac aattccgtat
     2581 tcaacagtgc cgattttaca acgcgaaggg cgtttcaaat ttgacctgac ggcgggcgat
     2641 tttcgtagcg gtaatagtca gcaatcatcg cctttctttt ttcagggtac ggcactcggc
     2701 ggtttaccac aggaatttac tgcctacggc gggacgcaat tatctgcaaa ttacaccgcc
     2761 tttttgttag ggttggggcg caacctcggg aactggggcg cagtgtcgct ggatgtaacg
     2821 catgcgcgca gtcagttagc cgacgacagt cgtcatgagg gggattccat tcgcttcctc
     2881 tatgcgaaat caatgaacac tttcggcacc aattttcagt taatgggtta ccgctattcg
     2941 acacaaggtt tttataccct tgatgatgtt gcgtatcgtc gaatggaggg gtacgaatat
     3001 gattacgatt atgacggtga gcatcgcgat gaaccgataa tcgtgaatta ccacaattta
     3061 cgctttagcc gtaaagaccg tttgcagtta aatatttcac aatcacttaa tgactttggc
     3121 tcgctttata tttctggtac ccatcaaaaa tactggaata cttcggattc agatacgtgg
     3181 tatcaggtgg ggtataccag cagctgggtt ggcatcagtt attcgctctc attttcgtgg
     3241 aatgaatctg tagggattcc cgataacgaa cgtattgtcg gacttaatgt ttcagtgcct
     3301 ttcaatgttt tgaccaaacg tcgctacacc cgggaaaatg cgctcgaccg cgcttatgcc
     3361 tcctttaacg ccaaccgtaa cagcaacggg caaaatagct ggctggcagg tgtaggtggg
     3421 accttactgg aaggccacaa cctgagttat cacgtaagcc agggcgatac ctcgaataat
     3481 gggtatacgg gcagcgctac ggcaaactgg caggccactt acggtacgct gggggtcggg
     3541 tataactacg accgcgatca acatgacgtt aactggcagc tgtctggcgg tgtggtcggg
     3601 catgaaaatg gtataacgct gagccagcct ttaggggata ccaatgtttt gattaaagcg
     3661 cctggcgcag gcggtgtacg cattgaaaat caaactggca ttttaaccga ctggcgcggc
     3721 tatgcggtga tgccgtatgc cacggtttat cggtataacc gtatcgcgct tgataccaat
     3781 acgatgggga attccatcga tgttgaaaaa aatattagca gcgttgtgcc gacgcaaggc
     3841 gcgttggttc gtgccaattt tgatacccgc ataggcgtgc gggcgctcat taccgttacc
     3901 cagggcggaa aaccggtgcc gtttggatca ctggtacggg aaaacagtac cggaataacc
     3961 agtatggtgg gtgatgacgg gcaagtttat ttaagcggtg cgccattgtc tggtgaatta
     4021 ctggttcagt ggggagacgg tgcaaactca cgctgcattg cgcactatgt attgccgaag
     4081 caaagcttac agcaagccgt cactgttatt tcggcagttt gcacacatcc tggctcataa
     4141 aggaaattat caatgaagat aatctgcaga ttattattgg caatggcatg tttgtgtctg
     4201 gcaaacatat cctgggctac tgtttgtgca aatagtactg gcgtagcaga agatgaacac
     4261 tatgatctct caaatgtctt taatagcacc aataaccagc caggccagat tgttgtttta
     4321 ccggaaaaat ccggctgggt aggtgtgtca gcaatttgtc cacccggcac gctggtgaat
     4381 tatacatacc gtagttatgt cactaacttt attgttcagg aaactatcga taattataaa
     4441 tatatgcaat taaatgatta tctattaggt gcgatgagtc tggttgatag cgtgatggat
     4501 attcaattcc ctccgcaaaa ttatattcgt atgggaacag atcctaacgt ttcgcaaaac
     4561 cttccgttcg gggtgatgga ttctcgtttg atatttcgtt taaaggttat tcgtcccttt
     4621 attaacatgg tggagatccc cagacaggtg atgtttaccg tgtatgtgac atcaacgcct
     4681 aacgatccgt tggttacacc tgtttatacc attagttttg gtggccgggt tgaagtaccg
     4741 caaaactgcg aattaaatgc cgggcaaatt gttgaatttg attttggtga tatcggcgca
     4801 tcgttattta gtgcggcagg gccggggaat cgaccagctg gtgtcatgcc gcaaaccaag
     4861 agcattgcga tcaaatgtac gaatgttgct gcgcaggctt atttaacaat gcgtctggaa
     4921 gccagtgccg tttctggtca ggcgatggtg tcggacaatc aggatttagg ttttattgtg
     4981 gccgatcaga acgatacgcc gatcacgcct aacgatctca atagcgttat tcctttccgt
     5041 ctggatgcag ctgcggcagc caatgtcaca cttcgcgcct ggcctatcag tattaccggt
     5101 caaaaaccga ccgaagggcc gtttagcgcg ctggggtatt tacgcgtcga ttatcaatga
     5161 ggtacggaga atgagaagag tgctctttag ctgtttctgc gggctactgt ggagttccag
     5221 tggatgggca gttgaccctt taggaacgat taatatcaat ttgcacggta acgttgttga
     5281 tttctcctgt accgtaaaca cagcggatat tgataagacg gtagatttag gcagatggcc
     5341 tacgacacaa ctactgaccg ctggcgatac cacggcactc gtccctttta gcctgcggct
     5401 ggagggatgt cctccgggtt cagtcgcgat tttatttacg ggaacgccgg catccgatac
     5461 caatctgctg gctctggatg atcccgcaat ggcacaaacc gtcgccatcg aattacgtaa
     5521 tagcgatcgc tcccggctcg cactggggga ggcgagcccg actgaggaag tagatgcaaa
     5581 tggcaatgtc acactaaact tttttgccaa ttatcgagcg ttagccagcg gtgttcagcc
     5641 aggtgtggcg aaagcggatg cgatatttat gatcaattat aattaatatt atattaattc
     5701 atataatttg gcgtagtcga taagctctac aattgaatgc aatcctagct tgccataaat
     5761 attagattta tgcgcactaa ctgttttatt gctaagtaat aacttatcgg caatttcttt
     5821 attagataat ccgctgacca gataacgtaa tatcgtcact tcacgattag atagcacagt
     5881 gatcgttgaa ctattcgtgc tacatttatt gctttttata tagttgagcg tttcgctggg
     5941 aaaaaaagtg tatccggaga ggatcatctg aacggcatga aaaatatcat tctgatcatt
     6001 gcatttactg acaaaaccgt tagcaccagc ttgtatcgct ctgccagcat aaaagcattc
     6061 tgatttcgat gataaaaata atactttcac tgtgctctgg atttgtttga tccttttcag
     6121 gaaggtaaaa ccgtctgttc cgggcaagtc tatatccata atgattaaat caacaggacg
     6181 ggttcggaga taatcgatgg ttatgcgata atcatccgtt ttcaggacaa tttgcaattc
     6241 actgtttttt tgcaacagaa cttcaataga cattctgatg ataggatgag tatccataat
     6301 gatcaccgac gttggtttca tagttaccag tctcatagga tcggacaatt ttccgttagg
     6361 gcgggaaatt gtactttgat acatgaaaat acgggttttc ttgattcaga cgcgcagcgg
     6421 tgtacgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg tcgttcactt
     6481 gttcagcaac cagatcaaaa gccattgact cagcaagggt tgaccgtata attcacgcga
     6541 ttacaccgca ttgcggtatc aacgcgccct tagctcagtt ggatagagca acgaccttct
     6601 aagtcgtggg ccgcaggttc gaatcctgca gggcgcacca ttacaattca atcagttacg
     6661 ccttctttat atcctccata attccacagt gggacatatt tgggacatta tcaccaaaaa
     6721 tgtcgtctat tttccttgca tgctctgtca aatgattagg cgcaaggtga gcatacctac
     6781 gaaccatttc tatggactcc catccgccca tttcctgaag cacagataat gggacgcctg
     6841 actggatcag ccagcttgcc caggtgtgtc tgaggtcatg gaaacggaaa tcttcaattc
     6901 ctgcacgacg acaagctgat agccatgatg tcttgctgtc gatgcgcatc ttcctgaccg
     6961 caggcgttga tgttccatct gctcgcttag ccgccttggt atgtacaaac acccatttgt
     7021 gatgcttgcc tatttgatca cgcaacactt tacaggcggt atcgttcagc gccacaccaa
     7081 tggcgcggtt tgatttgctc tcttctggat tcacccaggc aactcgtcgc tgcatgtcga
     7141 tttgttgcca ttccagattt atgatgttcg actttctcag accagttgcc agcgcaaact
     7201 tgacgacaga tttcagtggt tcggggcact catcaataag gcgttttgct tcctcctttt
     7261 ccagccatct gactcgcttg tttctgaccg ctggtatctt gatgacaggc gctttttcca
     7321 gccacttcca gtcgcgttct gcagcacgga gaatggcctt tatcatggca agatgctttg
     7381 cctttgtctg agttgatact ggctttggtt cataaacagg cggttcttta cctttcctga
     7441 tggcggcctg aactttctgt ttccatattt ctttcgtctt tctgttatgc attctgctta
     7501 cagcagagta aatctttgcc tccgagatat ctttaagcct tataccctca aaatgttcaa
     7561 gccagaactc aatccggctt ttatctgaat cgagagattt tttatcagct ttttcctcaa
     7621 gccatcttag gcaggcctct tcaaaagtga catcaggtaa atcccctagc ttttctactc
     7681 gccagagttc tgcttttcgc ttgtcgtgca actcctgagc ttgccgcttg tcctttgtgc
     7741 caagagattc cttaattcgt ttcccgcccg ggagcgaata cgaggcatac catatttcat
     7801 ttctgcggaa gagtgacatt ttctttcctc tgttatgcca tcacccgcgc tcacttggac
     7861 agtatgcagc ggagactgaa gcgccgcaat gcaagcttgc cgtgttgtga ggtaaggaga
     7921 ttttggcttg gttggatctt tacgtgttgc ctgtaggcgg cctgttcgta tccagttggt
     7981 ggcggttggt ctggatatct taagaaactg acaggcctca tcgagtgtga ggctgtatgg
     8041 ctccattatt tcacctcttg ctgtgtcatt gttgaaaaat ggataccagc tcgttgctgc
     8101 cagacgatcc aaccgagagt catatcccat gccatgtatt cgttatcgcc gttttttact
     8161 ctccgacgat ctactaagtc accgaaacgc ttttccatga ataattcata ggcttcgcgt
     8221 tcatctggct ctacttccag agatacgagt gcgatttcat aagcacggcg ctcaatatcg
     8281 tctcgaacct ctaggctgct gattcgttct ttgatttctt taatcagttc tttattggta
     8341 aatgtggtca ttatgctcca gcctccggtg cttttggcat tactgcccag tgagtgatat
     8401 tgacgttttc aaggtccccg acctgaaatg tccactgcca ttctccggtt tctttttgtc
     8461 cccaggtgta ccagagagaa cgccagccaa tcagccagcc ttctccatta gcatcaaata
     8521 acagaacact ttcatttgct ggtggcagtt cagctgacac tggtattatt ttgttttcca
     8581 gtgccgcaca tttagcttca agcgcgtcga atttacgtaa caggtactca gcatttgttt
     8641 cattcacttt cagatctcgc ggtacacatt tcccgcgaag aaacccttcc atttcgaaaa
     8701 cattcatgcg catttgcgta actccgataa atcgttaaaa cgttccataa acatcccgta
     8761 ggcatgaccc ggtgccagtg gaatcacgtt gaacatctct gttgccggga tgccttccag
     8821 tacaggccag aaagagccat catcaagccc gagatcgcgg cgttcggttg ccagcatgat
     8881 gagatcggca tatttcacgg gtgtactcat aactgggggt aacccgtatt cctcacggat
     8941 tacggcgtct attttttctt ccatccgttt atagtcagga agaaggcgtt tcagtggtgc
     9001 gggaatgtcc tggcaatacg cttctgttgc atcatgcatt aacgcttcaa aagcaaattc
     9061 ctgcggcacc agctggctgc aaagaaccgc atgttgggcg acgctgtaga agtgcgaaag
     9121 atgaccggca aagcgacaga tatttgaaag ggaaaccgcg atatcgttaa tatcgatgtc
     9181 gtctttattt atcttgtcat aataaaaatg cttcccggaa aaagttttaa taaatgacat
     9241 tttgttctcc acgtatatgc actgcaccgc gctgaattct ggtaaaagga agccctcacc
     9301 atccggtgat tattgagtta attacgtttc cataaatgcc cccgcagggg catttgcagt
     9361 aatgaaatca ggcggtgaaa gtaccaataa aggtttctac tttgctgtct ttgaatttct
     9421 caacaagcag atcacgaaat tcgttagcca tttcttcctg caccgcttcc agctgaataa
     9481 tgcgcagaac cagtacagga cgatcgccag tgataatgct gaggcgtaat ttaaacggac
     9541 gttctttcag accttcaaac ggaacgcatt taaattcaaa tgccactggc ataatgtctt
     9601 tggtcttcgc ttcgacagac tccatcaggg agcgtttgcc gctgaagtca ttatcttcaa
     9661 aatcagcggt ctggtttgct tcaatcgtga ttttacggac tgccgcagcc gcttttgttg
     9721 cttgaatggc gtcaccatta gcatcaaagc ccacaaggta gtcggcccag tcttcgatcc
     9781 attctgccag tgacctctgg gagttacgct cgccgttaac agacaacaga gcagagaacg
     9841 gtgctgtctt tttcagtttg agagtggcag tgttatctgc gtgacctggc tcatcaatag
     9901 tacccaggtt aagcacactg acggcacgca tattatcggc atcgataaag cagcgggtgc
     9961 cttcatctgc aagatcttta gaataacggg taaaatcatc gatgctggca gtggaaagtg
    10021 caccacggaa acggaagcga tttaaattaa atttttccag atcatgaatg cggaaattct
    10081 caggcaatgc cacagcatcg gcaccaatct tactgataat ttcattaaca ccctgagcag
    10141 aaataagggc atggatttga ttaattgcgg ttgcgtctaa gttctgagac ataataagtc
    10201 ctcactatat aaagatattc agtgatgaga taaataatca gtttattacg aacgatatta
    10261 acgacctgct gcgcggagtt ttccgtcagg ttcaccggca agagtcagta attgtccctg
    10321 gtcttcctgc agaatagtca ggcgaccacc gcgattgaca tacatcggcg tttcggtggt
    10381 gtcttcttcg gaaattttcc cgcggttagt tgggcgaaca tatgagagtt tgtgtttgat
    10441 tttcacacgg ttctcatcaa acggttcgat ttccagattg agtgagacct tccctttggt
    10501 tttcgtgttc atcacaccgg aagcgacctc actgagaact gcgccgattt tggtttcaaa
    10561 tacgccgccg tccagctccc cgataaatgc ctgcacatca gtactgcgtt cgctagccat
    10621 tttgctgctc ctcatcatat cgaccctgca aggtcggttg gtttctccac aaaacagaga
    10681 agaacacctg cggtggcagc cgcccggatg gattgggtta tgagcccgtc gtccggtgat
    10741 gctcttctct gttttgtaaa aagagcggtg ccagccggaa gcaagtgtac aaactggtac
    10801 cgccaaagca gtggctgttg tggtgccggg tgcctcccgg tgcctggcga aggttgcaca
    10861 ccaggcgggt gggtatccac agaaggtcga ctgtcagcct caaccttaac ccgcgtgcgc
    10921 tgagccgcat tcaccacaac gctaaggatt ctctctggtt gaaaatactt agctgttatg
    10981 tgcctgcttt tagccacatc aggcgaggtg gacctggtta ttccccaaca acaaggattc
    11041 ggttaatctt gataccccca ataacatggt gattatttaa gatggacaaa tttgatagaa
    11101 gtgcacaaag gcagttgtta caaatactgt atgatgctca tccttatgaa atttctgatg
    11161 atgcgttgca gtctgttcgt gacgcatttg gagatgataa tgtcctaatt tcgaacttaa
    11221 tttatctcga agaacatggc ttgattaaga atgctcttga ttattacttg gatggcataa
    11281 atataaatat tcctgaatta agaattacta aagatggaat tgatttcatt cgtgatgatg
    11341 gaggtcttaa agctattctt gggattatga caataagatt gcatgatgag actctctgtg
    11401 aacttgaacg tatcatcaat agttctactt ctgccacgac tgaagataag aagaaattac
    11461 tctctcagct tcgaacgctt cctgccgacg ccataaaaca gctgacgatt cgattacttg
    11521 gtcaggggct tgaccatctg ccggacgtat ttcacgtaat tcaaaaagcc ctccattctc
    11581 tgtaaattct gattctggtc ggattagaca gaatcgtccc catccgacct gcttactaag
    11641 tagaatccag aattcgtctt gttggttggt gctgataaaa aatccattag gatgaacgta
    11701 agcacagaat atgaccatag aaccctcttt tgttacgggg atactaagat tgttaaagag
    11761 ctaagcgtcc tgtagggcgc ttttttgttg ctaacgaatc atccggtcat tcatacgcca
    11821 ccggcggcta cttcgtgggc gtcctgcctg tccgtgatag cttacattat gttgctactt
    11881 aaagtagcaa aaatcaacaa caaaaagtag atatttgttg ttgtctatga tttttctata
    11941 attaattgaa agttaaatgt ttttgctgat ttactatgct gtaaactgaa aatatcgcac
    12001 gaagttggag taggtattga gcatagtgaa ttatgaggag ggagtttttt atgagattaa
    12061 tcagggagaa agaagagtaa agcccggtta tgcggccggg cacatgcatt actttgtaag
    12121 ttctagaact cgttgagcca gccctttaaa ttgttcgtta aaggcgtctc tgcttgattt
    12181 cattgtattt aaggcatgcc caaattgccc tgaatcgcga atttgctgat cactgatgga
    12241 gaaaacaggt gtcgctagtt gttggcttat tgcgattaat gagttgaaat cggaaatatg
    12301 cgcaagatca taggctgata aatctccact gactgttgtt ttgttaattg catgttgcac
    12361 tttatctcgt ggaattatgc aggtaatttt ttctagattt ggcactaaga tcgaatcaac
    12421 agcatcacgg atagctgcca tccatttttc gaaagatttt acaggtgctc ctttacgcgg
    12481 cctgtatctc tgctgaatca tacctagaaa ctgcggtgca tttttaattg attgtgaaga
    12541 gttaccatca ttattttctt taaataatgc taattctttg tgccagcgag gtatattttt
    12601 tgagagagag cgaattgctt gccagcaaaa aaagtctgga gctacaggta cgataaaata
    12661 gtcacttgac atgagaacta cttcatttaa acctccaaca ttagggctga gatcataaag
    12721 aatgtaatca atattattct tcatggcgat tagctgtaat atttttggca ggttccctgg
    12781 tatatttctt gtagcaggaa taccagcagc aatttttaga gaaacactta tctgtgaatc
    12841 taagtccgaa acatctaaat gtccgggaag aagcagaagg ttttcatgca gtgtagggtg
    12901 taattttccc gtttcttttt caaggtatgc ttcaggttgt ccgccattaa tcaaatattc
    12961 tacaataggt ccaagagtta agttatctct gctagaataa aaagaatcta aaccctcatc
    13021 aatcttttca tagccaagaa ccattcctgt tagattacat tgcgaatcaa ggtcaaccat
    13081 caatactttc ttgccttcat ccgcaagtgc ccatccaaga ttgaaggttg ttgtggtttt
    13141 acttacccca cctttatgat taaaaaaaca aattgatttt gtcatatttc tctatccttt
    13201 cgaccccc
//
LOCUS       DAYPHN010000139        13178 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000139 DAYPHN010000000
VERSION     DAYPHN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.139"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            273..608
                     /locus_tag="ACG0BW_003672"
     CDS             273..608
                     /locus_tag="ACG0BW_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFK7260590.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            1068..1511
                     /gene="yjdN"
                     /locus_tag="ACG0BW_003673"
     CDS             1068..1511
                     /gene="yjdN"
                     /locus_tag="ACG0BW_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFK7260591.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELIYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1644..2432
                     /gene="phnC"
                     /locus_tag="ACG0BW_003674"
     CDS             1644..2432
                     /gene="phnC"
                     /locus_tag="ACG0BW_003674"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFK7260592.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2457..3473
                     /gene="phnD"
                     /locus_tag="ACG0BW_003675"
     CDS             2457..3473
                     /gene="phnD"
                     /locus_tag="ACG0BW_003675"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7260593.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3534..4313
                     /gene="phnE"
                     /locus_tag="ACG0BW_003676"
     CDS             3534..4313
                     /gene="phnE"
                     /locus_tag="ACG0BW_003676"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFK7260594.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4334..5059
                     /gene="phnF"
                     /locus_tag="ACG0BW_003677"
     CDS             4334..5059
                     /gene="phnF"
                     /locus_tag="ACG0BW_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFK7260595.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQMGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            5060..5512
                     /gene="phnG"
                     /locus_tag="ACG0BW_003678"
     CDS             5060..5512
                     /gene="phnG"
                     /locus_tag="ACG0BW_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFK7260596.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5509..6093
                     /gene="phnH"
                     /locus_tag="ACG0BW_003679"
     CDS             5509..6093
                     /gene="phnH"
                     /locus_tag="ACG0BW_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFK7260597.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNTPLVSQPEQATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            6093..7157
                     /gene="phnI"
                     /locus_tag="ACG0BW_003680"
     CDS             6093..7157
                     /gene="phnI"
                     /locus_tag="ACG0BW_003680"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFK7260598.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHTTGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            7150..7995
                     /gene="phnJ"
                     /locus_tag="ACG0BW_003681"
     CDS             7150..7995
                     /gene="phnJ"
                     /locus_tag="ACG0BW_003681"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFK7260599.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7992..8750
                     /gene="phnK"
                     /locus_tag="ACG0BW_003682"
     CDS             7992..8750
                     /gene="phnK"
                     /locus_tag="ACG0BW_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFK7260600.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8861..9541
                     /gene="phnL"
                     /locus_tag="ACG0BW_003683"
     CDS             8861..9541
                     /gene="phnL"
                     /locus_tag="ACG0BW_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFK7260601.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELILEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9538..10674
                     /gene="phnM"
                     /locus_tag="ACG0BW_003684"
     CDS             9538..10674
                     /gene="phnM"
                     /locus_tag="ACG0BW_003684"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFK7260602.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAQASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10674..11231
                     /gene="phnN"
                     /locus_tag="ACG0BW_003685"
     CDS             10674..11231
                     /gene="phnN"
                     /locus_tag="ACG0BW_003685"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFK7260603.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGIGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11218..11652
                     /gene="phnO"
                     /locus_tag="ACG0BW_003686"
     CDS             11218..11652
                     /gene="phnO"
                     /locus_tag="ACG0BW_003686"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFK7260604.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11654..12412
                     /gene="phnP"
                     /locus_tag="ACG0BW_003687"
     CDS             11654..12412
                     /gene="phnP"
                     /locus_tag="ACG0BW_003687"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFK7260605.1"
                     /translation="MSLTLTLTGTGGAQGVPTWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDSIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12447..12776)
                     /gene="yjdP"
                     /locus_tag="ACG0BW_003688"
     CDS             complement(12447..12776)
                     /gene="yjdP"
                     /locus_tag="ACG0BW_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFK7260606.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12845..13162)
                     /locus_tag="ACG0BW_003689"
     CDS             complement(12845..13162)
                     /locus_tag="ACG0BW_003689"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFK7260607.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGSHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2916 a         3969 c         3679 g         2614 t
ORIGIN      
        1 aataacgtct tccgatacgt ttaaaacgtc agaaagataa aaatatcatt tgaattaaaa
       61 aaagaacaag tagagcatta accttatctt aaataataaa tggaggcaaa aagattattt
      121 tctttttgcg tttcctttca aatgaaaacg atcgtcgtct aaaatcagca gtacccccga
      181 caaactcagg gattttgtgt ataattgcgg cctttttcgg caatctgccg ttttttggcg
      241 cttttgccct gctgactttt gaggaaatcc acatgtcatt accacactgc ccaaaatgca
      301 actccgaata cacctacgaa gataacggca tgtacatctg cccggaatgt gcctacgaat
      361 ggaacgacgc agaacctgca caggaaagcg acgagctgat cgttaaagat gctaacggca
      421 atctgctggc tgacggcgac agcgttacca tcattaaaga tctgaaggtg aaaggtagct
      481 cttcgatgct gaaaattggc accaaagtga aaaacatccg cctggttgaa ggcgaccata
      541 acatcgattg caaaatcgac ggttttggtc cgatgaaact gaaatctgag tttgtgaaaa
      601 agaactgatt gtattgtgat cggtaagccg gataaggcgc tcgcgccgca tccggcaacg
      661 atgccgactg cctgatgcga cgcttgcgcg tcttatcagg cctacaaatt cccgcaccct
      721 ccgtaggccg gataaggcgt ttacgccgca tccggcaaca gtgccgactg cctgatgcga
      781 cgcttgcgcg tcttatcagg cctacaaatt cccgcaccat ccgtaggccg gataaggcgt
      841 ttacgccgca tccggcaacg gtgccgactg cctgatgcga cgcttgcgcg tcttatcagg
      901 cctacaaatt cccgcaccct ccgtaggccg gataaggcgc tcgcgccgca tccggcaacg
      961 atgccgactg cctgatgcga cgctgaccgc gtcttatcag gcttccctct catctgtata
     1021 aatttcgaac tacacttaac tggcttctct taactgaggt caccatcatg ccgttaagtc
     1081 cctacctctc ttttgccggt aactgtgccg acgcaattgc ttattatcaa cgtacgttgg
     1141 gcgctgaact gatctataaa atcagcttcg gcgaaatgcc aaaatcagcg caggacagcg
     1201 ccgagaactg cccttccgga atgcaatttc ccgataccgc catcgcccac gccaatgtgc
     1261 gcattgccgg aagcgatatc atgatgagcg atgctatacc gtcaggaaaa gccagctact
     1321 ccggctttac gttagtgctc gattcgcaac aggtcgaaga agggaaacgc tggtttgaca
     1381 atcttgccgc caacggaaaa atcgaaatgg cctggcagga aactttctgg gcgcatggct
     1441 tcggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcgtc aaacaacaac
     1501 caacgcaata atccgccggg aggcccgccc tcccgcactg tcatcgaatt cccgttaact
     1561 cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc acgatggagc
     1621 cactttttta gggaggctgc atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa
     1681 ccttcaatca gcatcaggcg ctgcacgcgg ttgatctgaa cattcatcac ggtgaaatgg
     1741 tggctctgct tgggccgtcg ggttccggca aatccaccct tttacgtcac ttaagcggtt
     1801 tgattaccgg cgataaatct gccggtagcc atatcgagct gctgggccgc acagtccagc
     1861 gcgaaggccg cctggcccgc gatatccgca aaagccgcgc caacaccggc tacatcttcc
     1921 aacaattcaa cctggtgaat cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg
     1981 gcagcacgcc attctggcgc acctgtttta gctacttcac ccgcgagcag aaacagcgcg
     2041 cgttacaggc gctgacccgc gttggcatgg tgcattttgc ccatcagcgc gtttccaccc
     2101 tctccggcgg gcaacagcag cgtgtggcga ttgcccgcgc gctgatgcag caggcgaagg
     2161 tgattctggc ggacgaaccc atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg
     2221 acaccctgcg cgacatcaac cagaacgacg gcattaccgt ggtcgtcacg ctgcatcagg
     2281 tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgccagggg cacgtcttct
     2341 acgacggcag cagccaacag tttgataacg aacgctttga tcatctctat cgcagcatca
     2401 accgcgtcga agagaacgcg aaagctgcct gacatcccca tcattgagga aaacgaatga
     2461 acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc accctgttaa
     2521 gcccggcgca cgccgaagag caggaaaagg cgctgaattt cggcattatt tcaacggaat
     2581 cacagcaaaa cctgaaaccg caatggacgc cgttcttaca ggatatggag aagaagctgg
     2641 gcgtgaaggt caacgccttc tttgccccgg actacgcggg cattatccag ggaatgcgct
     2701 tcaacaaagt ggatatcgcc tggtacggca atctgtcggc gatggaagcg gtggatcgcg
     2761 ccaacggtca ggtcttcgcc cagacggtcg cggcggatgg atcgccgggt tactggagcg
     2821 tgttgatcgt caacaaagat agtccgatca acaacctgaa cgatctgctg gcgaagcgga
     2881 aagatctcac cttcggcaat ggcgatccta actccacctc tggcttcctc gtccccggtt
     2941 actacgtctt tgccaaaaac aatatctccg ccagcgactt caaacgcacc gtcaacgccg
     3001 ggcatgaaac caacgcgctg gccgtcgcca acaagcaggt ggatgtggcg accaacaaca
     3061 ccgaaaacct cgacaagctg aaaacctccg cgccagagaa gctgaaagaa ctgaaggtga
     3121 tctggaagtc gccgctgatc ccaggcgatc cgatcgtctg gcgcaaaaat ctgtccgaaa
     3181 ccaccaaaga caagatctac gacttcttta tgaactacgg caaaacgccg gaagagaaag
     3241 cggtgctgga gcgcctgggc tgggccccgt tccgcgcctc cagcgacctg caactggtgc
     3301 cgattcgcca gctcgcactg tttaaagaga tgcagggcgt gaaaagcaat aaagggctga
     3361 atgagcagga caagctggca aaaactagcg ccattcaggc gcagctggat gacctggacc
     3421 gcctgaacaa cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag taatgtgctc
     3481 gatcggtccc ctctcccaca gggagaggga gaaaacatta aacggagcaa tatatgcaaa
     3541 ccatcaccat cgccccaccc aagcgcagct ggttctcgct tctgagctgg gccattgttc
     3601 tcgccgtgct ggtcgtctcg tggcagggcg cggaaatggc cccgctcacg ctgattaaag
     3661 acggcggcaa catggcgacg ttcgctgccg acttcttccc gcccgatttc agccagtggc
     3721 aggattacct caccgaaatg gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg
     3781 cggtggttct ctccatcccc tttggcctga tgagcgccga aaacctggtg ccgtggtggg
     3841 tttaccaacc cgttcgccgc ctgatggacg cctgccgcgc cattaacgag atggtcttcg
     3901 ccatgctgtt cgtggtcgcc gtcggtctcg gtccgttcgc tggcgtactg gcgctgttta
     3961 tccacaccac cggcgtgctc tccaaactgc tttccgaagc ggtagaagca attgaacccg
     4021 gcccggtgga aggtattcgc gccaccggtg ccaacaagct cgaagagatc ctctacggcg
     4081 tgctgccgca ggtgatgccg ctgctgatct cctactccct ctatcgcttc gaatccaacg
     4141 tccgttcggc caccgttgtt ggcatggtcg gcgcgggcgg gatcggcgtc accctgtggg
     4201 aagcgattcg cggtttccag ttccagcaaa cctgcgccct gatggtgctg attatcgtca
     4261 cggtcagcct gctggatttc ctctctcaac ggttgcgtaa gcactttatc tgataagcga
     4321 ggcattgata tctatgcact tgtctacaca tccgaccagc tacccaacac gctatcagga
     4381 gatagccgca aaacttgagc aggagcttcg tcaacactac cgctgcggcg actatcttcc
     4441 cgccgagcag caactggcgg cgcgctttga ggtgaaccgc cacaccctgc gccgcgccat
     4501 cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct
     4561 gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt tttagccaga acctgctgga
     4621 tcagggcagc catcccacca gcgaaaaact gctgtctgtg ctgcgcccgg cctccggtca
     4681 cgtcgctgac gcgctgggga taaccgaggg cgagaacgtt atccacctgc gcaccctgcg
     4741 ccgggtcaac ggcgtcgcgc tctgtttaat cgaccactac tttgcggacc tcaccctctg
     4801 gccgacgctg caacgcttcg acagcggctc gctgcacgat tttctgcgcg agcaaatggg
     4861 gattgcgctg cgccgcagcc agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg
     4921 ccagcgtctt gaaatcccga atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg
     4981 tgacggtgaa agcagcccgg cggagtactc cgtcagcctg acgcgcgccg acatgattga
     5041 attcactatg gagcactgaa tgcacgcaga taccgcgacc cgccagcact ggatgtccgt
     5101 actggcacac agccaaccgg cggaactggc agcccgcctg aaggcgctga acatcactgc
     5161 cgactatgaa gtaatccgca ctgccgaaac tggcctggtg cagattcagg ctcgaatggg
     5221 cggcaccggc gaacgctttt ttgctggcga cgccacgctc acccgcgccg ccgtccgcct
     5281 gactgacggc acgctcggct acagctgggt gctggggcgc gacaaacagc acgccgaacg
     5341 ctgcgcgctg atcgacgcgc tgatgcagca atctcgccac tttcaaaact tatcagaaac
     5401 tcttattgcc ccgctggacg ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt
     5461 aaacgccagc cgggtcgact tctttacgat ggttcgcgga gacaacgcat gaccctggaa
     5521 accgctttta tgcttcccgt gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc
     5581 atgagcgagc cgggcgtgat tgtcgccctg catcagctca aacgcggctg gcagccgctg
     5641 aatatcgcca ccaccagcgt gctgctgaca ctggccgata acgacacgcc ggtgtggctt
     5701 tctgcgccat taagtaacga tatcgtcagc cagagcctgc gttttcatac caacacgccg
     5761 ctggtcagcc agccggaaca ggcgaccttc gcggtaacgg atgaggcgat ttccagcgaa
     5821 cagctcaacg ctctttccag cggcaccgct gttgcgccgg aagcgggcgc gacgctgatt
     5881 ttacaggtcg ccagcctgag cggcgggcgc atgctgcgcc ttactggcgc gggtattgcc
     5941 gaagaacgaa tgatcgcccc gcagctgccg gagtgcattc tgcacgaact taccgagcgc
     6001 ccgcacccgt tcccgctcgg catcgacctg atcctgacct gcggcgagcg gctgctggct
     6061 atcccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaga
     6121 aggcgatcga cgccgcccac gccctgcaag agagccgacg ccggggcaat accgatttgc
     6181 ctgaactgag cgtcgcccag attgaacagc agcttaacct cgcggtagat cgcgtgatga
     6241 ccgaaggcgg cattgccgac cgcgaactgg cggcgctggc gctgaaacag gccagcggcg
     6301 ataacgtcga agcaattttc ctgctgcgcg cctaccgtac cacgctggcg aagcttgcgg
     6361 taagcgagcc gctcgacacc accgggatgc gtctcgaacg gcgtatttca gcggtgtata
     6421 aagatattcc cggcggccag ctgcttggcc caacctacga ctacacccac cgcctgctcg
     6481 atttcaccct gctggcaaac ggcgaagcgc cgacgctgac cacagccgac agcgaacaac
     6541 agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg gctggcgaag tttgaagaag
     6601 atagcggcgc acagccggac gacatcaccc gcacgccgcc ggtttacccc tgctcacgct
     6661 cctcccgcct gcaacaatta atgcgcggcg acgaaggcta tttgctggcg ctggcctact
     6721 ccacccagcg tggttacgga cgcaatcatc cgttcgcagg cgaaatccgc agcggctata
     6781 tcgacgtgtc gattgtgccg gaagagctgg gatttgcggt gaacgtcggc gaactgctga
     6841 tgaccgaatg tgaaatggtc aacggtttta tcgacccgcc ggatgagccg ccgcacttca
     6901 cgcgcggcta cgggctggtg ttcggcatga gcgagcgcaa agcgatggcg atggcgctgg
     6961 ttgaccgtgc attgcaggct ccggagtacg gcgagcacac gacaggcccg gcgcaggatg
     7021 aagagttcgt gctggcgcat gccgacaacg tcgaagccgc aggctttgtc tcgcacctta
     7081 aactcccgca ctacgtcgat ttccaggccg aactggagct actcaaacgt ctgcaacagg
     7141 agcagaacca tggctaatct gagcggctac aactttgcct acctcgacga gcagaccaaa
     7201 cgcatgatcc gccgcgccat cttaaaagcg gtagcgatcc ccggttatca ggtacctttt
     7261 ggcgggcgcg agatgccgat gccgtacggc tggggaactg gtggtatcca gcttaccgcc
     7321 agcgtgattg gcgaaagcga cgtgttgaag gtgattgacc agggtgcgga tgacaccacc
     7381 aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg gggtaaacac cactgaacgt
     7441 acggacgatg cgacgcttat ccagacgcgt caccgcatcc ccgaaacgcc gctgaccgaa
     7501 gatcagatca ttatcttcca ggtgccaatc ccggaaccgc tgcgctttat cgagccgcgc
     7561 gaaacggaaa cccgcactat gcacgcgctg gaggagtacg gcgtgatgca ggtgaaactg
     7621 tatgaagata tcgcccgctt cggtcatatc gccaccacct atgcctatcc ggtgaaggtg
     7681 aacgggcgct acgtgatgga cccgtcgccg atcccgaaat tcgataaccc aaaaatggac
     7741 atgatgcccg ccctgcagct gttcggcgca ggacgcgaga agcgcatcta tgcggtgccg
     7801 ccgtttaccc gcgtggaaag cctcgatttc gacgatcacc cgttcaccgt tcagcagtgg
     7861 gatgagccat gcgccatctg cggatcgacc cacagctatc tcgacgaagt ggtgctggat
     7921 gacgccggaa accgcatgtt tgtctgctcc gataccgatt attgccgcca acagagcgag
     7981 gcaaaaaacc aatgaatcaa ccgttacttt cggtcaataa cctgacccac ctttacgcgc
     8041 cgggcaaagg ctttagcgat gtctcttttg atttatggcc gggggaagtg ctgggcatcg
     8101 tcggggaatc cggctccggg aagaccacgc tgctgaaatc aatttccgcg cgtctgacgc
     8161 cgcagcaagg ggagattcac tatgaaaacc gttccctcta tggcatgagc gaggccgacc
     8221 gccgtcgcct gctgcgcacc gaatggggcg tggtgcatca gcatccactc gacggcctgc
     8281 gccgccaggt gtcggcaggc ggcaatatcg gcgagcggct gatggcgacc ggggcgcgtc
     8341 attacggcga tattcgcgcc accgcgcaga agtggctgga agaggtggag attcccgcta
     8401 accggatcga cgacctgcca accacctttt ccggcggcat gcagcagcgt ttgcagattg
     8461 cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga tgaaccgacc ggcgggctgg
     8521 atgtatcggt acaggcgcgc ctgctcgatc tgctgcgcgg tctggtggtg gagctgaatc
     8581 tggcggtggt gattgtcacc cacgatctgg gcgtcgcccg cctgctggcg gaccgtttgc
     8641 tggtgatgaa acaggggcaa gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc
     8701 cgcaccaccc gtacacccag ttgctggtgt catcggtttt gcagaattga gccagtgctg
     8761 gatacggcgt aaacgcctta tccggccgac aaatgcgctc cccgtaggtc ggataagacg
     8821 cgtcagcgtc gcatccgaca cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt
     8881 cagtaaaacc ttcatcctgc accaacaaaa cggcgtgcgc ctgcccgtct tacaacgtgc
     8941 ctcgctcacc gtcaacgcag gcgaatgcgt ggtgcttcac ggccactccg gcagcggaaa
     9001 atcaaccctg ctgcgctcgc tgtacgccaa ctatctgccc gacgaagggc aaatccagat
     9061 caaacacggt gaggaatggg tagacctggt caccgcgcca gcccgcaaag tggtggagat
     9121 ccgcaaaacc accatcggct gggtgagcca gtttctgcgc gtcatcccgc gtatttcggc
     9181 actggaagtg gtgatgcagc ctctgctcga taccggcgta ccgcgtgaag cctgcgccgc
     9241 aaaagccgcc cgattactga cgcgcctgaa cgtgcctgaa cgcctgtggc accttgcacc
     9301 atcgaccttc tccggcggcg aacagcagcg cgtcaacatc gcccgtggct ttatcgtcga
     9361 ctaccccatt ctgctgctcg acgaacctac cgcctcgctg gacgccaaaa acagcgccgc
     9421 ggtggtggaa ctaattctcg aagctaaagc ccgtggcgca gccatcgtcg gtatcttcca
     9481 tgacgaagcc gtacgcaatg acgtcgccga ccgcctgcac ccgatgggag cctcttcatg
     9541 attatcaata acgtcaaact ggtgctggaa aatgaagtgg tgcacggttc actggaagta
     9601 caggatggcg aaattcgcgc ctttgccgaa agccagagcc gccagccgga agcgatggac
     9661 ggcgaaggtg gctggctact gcccggactg attgagctgc ataccgacaa cctcgataaa
     9721 ttcttcaccc cgcgcccgaa agtcgactgg cccgcccatt cggcgatgag cagccacgac
     9781 gcgctgatgg tggcaagcgg catcaccacc gtactggacg ccgtggcgat tggtgatgtg
     9841 cgcgacggcg gcgatcggct ggagaatctg gagaagatga tcaacgccat cgaagagacg
     9901 cagaaacgcg gcgtcaaccg cgccgagcac cgcctgcacc tgcgctgcga actgccgcac
     9961 cacaccacgt tgccgctgtt tgaaaaactg gtgcagcgcg aaccggtgac gctggtatcg
    10021 ctgatggacc actcgccggg ccagcgccag ttcgccaatc gcgagaagta tcgcgaatat
    10081 tatcagggca aatattccct caccgatgcg cagatgcagc agtacgaaga agagcaattg
    10141 gcgctcgccg cacgctggtc gcagccaaat cgcgaaacca tcgccgcaat gtgccgcgcg
    10201 cgacacattg cgctcgccag ccacgatgac gccacgcacg cccacgttgc cgaatcccac
    10261 cagcttggca gcgtgatcgc cgaatttccc accacgttcg aagcggcaca agcctcgcgc
    10321 aagcatggca tgaacgtgct gatgggcgcg ccgaatatcg tgcgcggagg ttcgcactcc
    10381 ggcaacgtgg cggccagtga actggcgcag cttggcctgc tggatatcct ctcttccgac
    10441 tactaccccg ccagcctgct cgatgcggca tttcgcgtcg ccgatgacga gagcaaccgc
    10501 tttacgctgc cgcaggcggt gaagctggtg actaaaaatc cggcgcaggc gcttaatctt
    10561 caggatcgcg gagtgattgg cgagggcaaa cgcgccgacc tggtgctggc gcatcgcaag
    10621 ggcaaccaca ttcatatcga ccacgtctgg cgtcagggta aaagggtgtt ctgatgacgg
    10681 gaaaactgat ttggttaatg ggggcctctg gctccggaaa agacagtctg ttgacggaac
    10741 tccgccagcg ggaacaaact cagctactgg tagcgcatcg ctacatcacg cgcgccgcca
    10801 gcgccggaag tgaaaaccat atcgccctga gcgagcagga gttttttacc cgcgcggggc
    10861 agaaccttct ggccttaagc tggcacgcca acggcctgta ttatggcatc ggcgtcgaga
    10921 ttgatctctg gctgcacgct ggatttgacg tgctggtcaa cggctcacgc gcgcatttgc
    10981 cgcaggcgcg ggcgcgctat caatcggcgc tgctgcccat ttgcttacag gtttcgccgg
    11041 agatcctgcg ccagcgcctg gaaaaccgtg gccgtgaaaa cgccagtgaa attaacgccc
    11101 ggctggcgcg cgccgcccgc tatactcccc aggattgcca tacgctcaat aatgacggca
    11161 gcctgcgcca gtcggtcgac acgctgctga cgctgattca tcagaaggag aaacaccatg
    11221 cctgcttgtg agcttcgccc ggccacgcag tacgataccg acgcagttta cgcgctgatt
    11281 tgtgagctaa aacaggcgga gtttgaccac catgcgtttc gcgtggggtt taacgccaat
    11341 ctgcgcgatc cgaacatgcg ctaccatctg gcgctgctgg atggcgaagt tgtcggcatg
    11401 atcggcctgc atttgcagtt tcatctgcat catgtcaact ggatcggcga aattcaggaa
    11461 ctggtggtga tgccgcaggc gcgcggtctg aacatcggca gtaaattact ggcatgggca
    11521 gaagaagaag cccgccaggc cggggccgaa atgaccgagc tttccaccaa cgtgaagcgc
    11581 cacgacgcgc accgttttta cctgcgtgaa ggctacgagc agagccactt ccgcttcacc
    11641 aaggcgctgt aatatgagcc tgaccctcac acttactggc accggaggcg cacagggcgt
    11701 tccgacatgg ggctgcgagt gcgtagcctg cgccagagcg cggcgctcac cgcagtatcg
    11761 ccgccagcca tgcagcggcg tcgtgaagtt taacgacgcg atcaccctga tcgacgccgg
    11821 gctgcacgat ctcgccgatc gctggtcgcc cggatcgttc cagcagtttt tgctgacgca
    11881 ctaccatatg gatcacgtgc aggggctgtt tccgctgcgc tggggcgtgg gcgattcgat
    11941 cccgatctac ggcccgccgg atgaacaggg ctgcgacgat ctgtttaaac atccgggcct
    12001 gcttgatttc agccacacga tggaaccgtt tgtggtgttt gatttacagg ggttacaggt
    12061 cacgcccctg ccgctcaacc actcaaaact gaccttcggt tatctgctgg aaacggcaca
    12121 cagccgcgtg gcgtggttgt ctgacaccgc tggcttgccg gaaaaaacgc tgaaattttt
    12181 acgcaataat cagccacagg taatggtgat tgattgcagc cacccaccgc gcgaggatgc
    12241 accgcgtaat cactgtgatt taaacaccgt gctggcgctg aatgaggtta tccgctcgcc
    12301 acgggtgatt ctgacgcata tcagccacca gtttgatgcg tggctgatgg aaaacgcact
    12361 gccgtcgggg tttgaggcgg ggtttgatgg gatggagatt ggggtggcgt gatgggtgag
    12421 gggcgatgcc tgctctaaac tcaacatcaa cgcccatact catcttccat tctccgctct
    12481 tcatcctcca gttgccgacg ctcctgatca agctggcgct ggcgatcgtc cagctgcctg
    12541 cggcgatctt caaactggcg gcggcggtcg tcatattgtc tgcgccgatc gtcgttcact
    12601 tcacgctgcc agccgtcgtc gcgcgaatct tcatagtctc gcccacggtc agggttataa
    12661 gcatcgttaa tcgcctgctg aatattgcca atggtgtcgt cgataatatc tgcctgggcc
    12721 ggaacggtgg acagcgtgag cagggtgaat aaaagaaata gcggaaagcg tttcattagc
    12781 caacctcaaa aagaaactct atccacatta gtcattactc atccatgcaa gtagtggatg
    12841 aatctcaatt tctccgctgc tctattgccg taatcgcctc cacgcgttgt tgatgactac
    12901 cgccttcgta ctgtgcgccc agccacgcat cgacaatcat ttttgccagt tcgaggccaa
    12961 ccactcgtga accaaaagcc agtgcaactt cactggcgta atgcggataa tcagtgcgct
    13021 ctgacgacct ggtttcttta tcaatcccct cactcattga ccatgaataa aaggctggca
    13081 cagcaacgca gaatcctgcg gtgccagcga catgaccgcc cattgctccg gtacgttttt
    13141 taataaaaaa caccggttgc attgattaat tgtgctca
//
LOCUS       DAYPHN010000140        13105 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000140 DAYPHN010000000
VERSION     DAYPHN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.140"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            68..352
                     /locus_tag="ACG0BW_003690"
     CDS             68..352
                     /locus_tag="ACG0BW_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFK7260608.1"
                     /translation="MITYHDAFAKANHYLDDADLAVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLSGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(390..2132)
                     /gene="ggt"
                     /locus_tag="ACG0BW_003691"
     CDS             complement(390..2132)
                     /gene="ggt"
                     /locus_tag="ACG0BW_003691"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFK7260609.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSASAAPPPPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALEK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLKK
                     GDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            2252..2692
                     /locus_tag="ACG0BW_003692"
     CDS             2252..2692
                     /locus_tag="ACG0BW_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFK7260610.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            complement(2679..3422)
                     /gene="ugpQ"
                     /locus_tag="ACG0BW_003693"
     CDS             complement(2679..3422)
                     /gene="ugpQ"
                     /locus_tag="ACG0BW_003693"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFK7260611.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTSPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(3419..4489)
                     /gene="ugpC"
                     /locus_tag="ACG0BW_003694"
     CDS             complement(3419..4489)
                     /gene="ugpC"
                     /locus_tag="ACG0BW_003694"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFK7260612.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPSVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(4491..5336)
                     /gene="ugpE"
                     /locus_tag="ACG0BW_003695"
     CDS             complement(4491..5336)
                     /gene="ugpE"
                     /locus_tag="ACG0BW_003695"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFK7260613.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(5333..6220)
                     /gene="ugpA"
                     /locus_tag="ACG0BW_003696"
     CDS             complement(5333..6220)
                     /gene="ugpA"
                     /locus_tag="ACG0BW_003696"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFK7260614.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRLYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(6318..7634)
                     /gene="ugpB"
                     /locus_tag="ACG0BW_003697"
     CDS             complement(6318..7634)
                     /gene="ugpB"
                     /locus_tag="ACG0BW_003697"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFK7260615.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHITMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(8032..8745)
                     /gene="livF"
                     /locus_tag="ACG0BW_003698"
     CDS             complement(8032..8745)
                     /gene="livF"
                     /locus_tag="ACG0BW_003698"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFK7260616.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(8747..9514)
                     /gene="livG"
                     /locus_tag="ACG0BW_003699"
     CDS             complement(8747..9514)
                     /gene="livG"
                     /locus_tag="ACG0BW_003699"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFK7260617.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(9511..10788)
                     /gene="livM"
                     /locus_tag="ACG0BW_003700"
     CDS             complement(9511..10788)
                     /gene="livM"
                     /locus_tag="ACG0BW_003700"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFK7260618.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(10785..11711)
                     /gene="livH"
                     /locus_tag="ACG0BW_003701"
     CDS             complement(10785..11711)
                     /gene="livH"
                     /locus_tag="ACG0BW_003701"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFK7260619.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(11759..12868)
                     /gene="livK"
                     /locus_tag="ACG0BW_003702"
     CDS             complement(11759..12868)
                     /gene="livK"
                     /locus_tag="ACG0BW_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFK7260620.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         3147 a         3759 c         3303 g         2896 t
ORIGIN      
        1 gcgtcttttc tcaaagtata acgcaagaat aataatataa cgatcaacaa taatatttat
       61 tataattatg attacttatc acgacgcatt cgcgaaagcg aaccattacc ttgatgatgc
      121 agatctcgcc gtcgtcatta ctctacatgg acgctttagc cagggctggt atttctgttt
      181 cgaagcacga gaatttctcg aaactggtga tgaggccgcg cgtttatccg gtaacgcacc
      241 ttttattatt gataaagaca gtggtgaaat tcattccctt ggaacggcaa agccgctgga
      301 agaatatcta caggattatg aaataaaaaa ggctaccttc ggcttgccct gacaatacgg
      361 ccctcttccc acgaagaggg ccgctaacct taataccccg ccgttaaatc atccaccgag
      421 cgcggatcgg atgcaccgta cagctcaccg tccggcccaa ccataatgct ttgtgtactg
      481 cccatcgcct ctttcagcgc cactttctga ccttttgctt ccagcagctt gagcgtatcc
      541 gggctaaacc ctttttcgac acgcagctcg tccggcaacc attgatggtg gaaacgcggt
      601 gcattggtcg cttcagcgac attcatgcca taatcgatgc tattcaccac catttgcagc
      661 acggtggtaa taatccgact cccgccaggg ctaccggtaa ccagccaggt tttaccgtct
      721 ttcaccacaa tggttggtga catcgacgac agcgggcgtt tgttcggccc gaccgcgttg
      781 gcatcaccgc ccaccagccc gtaaacgttc ggtacgcccg gtttggcgga gaaatcatcc
      841 atctggttat taagcagaat accgctctcg cccgcgacaa tgcccgtacc gaaggtggtg
      901 ttcagcgtat aggtcaccgc caccgcgttg ccgtctttat ccaccactga gtaatgggta
      961 gtttgattac tctcataagg cgcaagcttg ccggggcgaa tttcgctgga tggcttcgct
     1021 ttattgatat caatttgatc ggcaatagat ttggcatagg ctttattggt cagcgcctgc
     1081 cacgggactt tcacaaaatc cggatcgcca agatattccg agcggtcggc gtaggcgtat
     1141 ttctccgctt ctgccatgat ttgcatcgca tcggcgctgc caaagccgta tttctgcata
     1201 tcgaagtttt ccaggatatt gaggatctgc acgatatgaa tcccaccgga agatggcggt
     1261 ggcatcgagt aaacctgata cccgcgatat tcgccgctaa tcggtgtacg ttcgaccgct
     1321 ttatacgccg ctaaatcttc tttagtgatc aagccaccgt ttttctgcat ctcctgagcg
     1381 atctgctccg ctattgtgcc tttgtagaat tcgtccgggc cgttttcggc aatcatctcc
     1441 aggctctttg ccaggttcgc ctgcaccagc ttgtcgccct ttttcagcgg cgcgccctct
     1501 ttccagaaga tagccttgct gttttcgtga ttcggcaaca cttcgctacc gtaggttttg
     1561 agatcgtcag ccagcgcgtc attaacgata aaaccatcgc gtgccagttt aaacgccggc
     1621 tgcaccactt tatttaacgg catggtgccg tacttctcca gcgccagcga gaaacctgcg
     1681 accgtacctg gcgtaccgga ggccagatgc gaagtgagtg attttttgct gtccgggttg
     1741 ccctggtcat cgaggaacat atcgcgggtc gctttggcgg gtgccatttc gcggaaatcg
     1801 atagcggtgg tattgccatt tttcgagcgg atcagcataa aaccaccacc gcccagattc
     1861 cctgcctgcg gatgcgttac cgccagcgcg tagcccacag ccacggcggc atcaacggca
     1921 ttcccgccct ccttgaggat atccaccccc acctgagtgg cagtagcatc tacagacgcc
     1981 accatcccct gtttcgcgcg taccggatgg aagacatctt cctccacgcc ataagaaaca
     2041 ggtggcggag gaggcgcggc ggaggcgcta aaacaacttc ctgagagcag agcagcaatg
     2101 gccacccggc gtaaaaacgt cggttttatc atcgttattc tccagagatt aaggggcaac
     2161 cccagctaag cctggtatat aactctgaat taatcatcgt tttgccggga agcgagtaaa
     2221 cttaaaggat atcttcagag gaggatacgc gatgaaacga cttctgattc ttacggcact
     2281 cctgccgttt gtcggctttg cacagcccat taatactctg aacaacccca accagccggg
     2341 atatcagatc cccagccagc agcggatgca aacccaaatg caaacgcagc aaatccagca
     2401 aaaagggatg ctgaatcagc aactgaaaac gcaaacgcag ctgcaacaac agcatttaga
     2461 aaaccagata aacaataatt ctcagcgggt gttgcagtca cagccggggg agcgaaatcc
     2521 cgcccggcag caaatgctgc ccaacaccaa tggcgggatg ttaaacagca accgtaatcc
     2581 ggatagttcg ttgaatcagc agcatatgtt gccggagagg agaaacggcg acatgctgaa
     2641 tcagcccggc acgccgcagc ctgatatacc gttgaaaact attgggccgt aaagttcgga
     2701 ccaatcacat caatcgcatc ggtacagatg caatccacgc cccagcgcag caactctgcc
     2761 gcgcgctggg gtttattgac ggtataaacc agaatccgta atccggcgtc tttcaactgc
     2821 atcactcgcg ctttatcgag caacttatga ttgagatgaa tagagacgca gcccagccgc
     2881 gcggtcagtt ctcgccagtc gtcgcgccac tcatcaagca ataaaccgcg cggcagttct
     2941 ggtactgctt gttgcgcggc ttctaaagca tcaatctcaa acgatgacag cagcggcgac
     3001 gtcataccag cccacagttc gcgtgcagcc agcgccacca ttttgcccgt taatggcccg
     3061 gtgccagtgg tgggcttgat ttcgatattc gccatcatcc cgtgttcacg acagcgttcc
     3121 gccacctgcg aaagcaacgg cagcggctca cctttaaacg ctttgctgta ccaactgccc
     3181 gcatccacgc gcagtaaatc ctgccagttc agttcacccg cgacgcccca gccgttgctg
     3241 gtgcgttcga gattgtcgtc atggagcaag aagatctcgc catccttcga taacttcgcg
     3301 tcaaattcga tcatcttatg accgtatttt gcccccacgt cgattgccgc cagggtgttc
     3361 tccggggcca gtttaccgcc gccacgatgg gcgacgatgc ggggataagg ccagttactc
     3421 atactcgttg tcctgtttca ccatcaaaaa gatgcagctg attttccggc agatgcagcc
     3481 acagcgtgct gcctgccgtc gggcgctcct gatgcgccag tcgcaccacc agcttctgtt
     3541 cgccccagcg tccgtgcgcc aggttatctg cgccgaggat ctccagcgtg tccatcacca
     3601 gcggcacgcc accttcagcc tgcgaacgta gcgtaatatg ttccgggcga atgccgagag
     3661 tcattttacg tccagcatac tgacggtagc caccgtttag cggcagcgcg ataccgccgt
     3721 ccagttcgaa atgcgtgcct tcgttattca cacgccctgc cagcagattc atcgccgggc
     3781 tgccgataaa actcgctaca aacaggctgg cgggcttttc gtacacttca actggcgtgc
     3841 caatctgttc ggcaacgccg ccgttcatca ccattactcg ctgggcgagc gtcatcgcct
     3901 caacctgatc gtgggtaacg tagagtgaag tcgttttcag gcgacggtgc aactgttgca
     3961 gttcaagacg catctgcacg cgcagcttgg catcgaggtt agagagcggc tcatcaaaca
     4021 agaacaccga cggatcgcgc acaatcgcgc ggcccatcgc cacacgctgg cgctgaccgc
     4081 cggaaagctc gcgcgggcga cgtttgagca gaccgtccag ctccagaatg cgcgccgctt
     4141 ctttaacgcg ctcggcaatt tgctgcttgc ccatgccgcg aattttcagc ccccacgcca
     4201 tgttttcttc gacgctcata tgcggataaa gcgcgtagtt ctggaacacc atcgcaatcc
     4261 cgcgatcttt cggctccatt tcggtcaccc gctggtcgtt gatccagata tcgccttccg
     4321 tcacccgctc caacccggca accatgcgca gcagcgtcga tttcccgcag ccagacggcc
     4381 cgaccatcac gataaattcg ccatccgcca catcaagggt cagcggttta atcacctggg
     4441 ttttgccatc ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca
     4501 ctatcgacca ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac tactggtggg
     4561 atcagggtta acaacatcgc cgccatcacc gagttccatt ccgtggtgcc ttcgcctgtg
     4621 gcgatcatcc ctttaatccc tgccacggtg gtgcctagat ccacgtcggt aataatcaac
     4681 aacggccaca aatactggtt ccagccgtag ataaaggtga tcacaaacag cgccgccaga
     4741 ttggttttgg acagcggaaa aacgatgtcg caaaagaaac gcattggtga tgcgccgtcg
     4801 atccgcgcgg cttccaccag ctcatccggc agcgtcataa agaactggcg gaacaggaag
     4861 gtggcggtcg ccgaggccat cagcggcagc gttaagccgg cgtagctgtc gagcatcttc
     4921 agattggcga tgacttccac cgtcgggaaa atacgcactt caaccggcaa catcagagta
     4981 ataaaaatca tccagaagaa gaggttacgc agcggaaaac gaaaccagac aatggcaaat
     5041 gccgagagca tcgagacggt aattttgccg agcgtaatgc taaacgccat cacaaagctg
     5101 ttaagcaaca tccgccagaa cggcgcgcta ttcgtaccta ccccgtttac ccagatgttg
     5161 tggatatttt ccagcagatg cgtgccgggg atgagcgtca tcggcgcggc gtagacggcc
     5221 tgtttatcca gcgtcgccgc gacaaacgcc acgtacagcg ggaagaggat caccgcgatc
     5281 ccgaggatca gcatggtatg gctgaatatt gtcagccacg gacggttctc aatcattggt
     5341 aacgcacctt gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca
     5401 tcaacaccac cgactgcgcg gcagacgaag ccagatccag tccggtaaag ccttcgcggt
     5461 aaatcttata aatcagcgtc gtggtggcct gaaccggccc gccggacgtg gcggcgtcga
     5521 tcaccgggaa ggtgtcgaag aaggcgtata ccaggttcac caccagcagg aagaaactca
     5581 ccggagcgat aagcggcagc gcaatcttaa agaagcggcg aatcgggcct gcaccgtcga
     5641 tggctgcggc ttcgatcaac gaacggggta tggattgcag cgcggcatag aagaacagga
     5701 agttgtagct aatttgcttc cacaccgagg caaacaccac cagaaacatc gcctgaccgc
     5761 tgttttgcgc gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc
     5821 gaccggggtt aaacaggaag atccataata cggcggcaac ggcgggagcc acggcatacg
     5881 gcagcagcat taaggtttga tataaacggc tgccgcgcac gatgtactcc accagcgctg
     5941 cgaagaacag cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg
     6001 tccagaagga gtcgagatag tagctgtcat gaaacaacgt gacgaagtta tccaggccga
     6061 caaactggct ggagaaacca aacggatcga cgctttgtag cgagtaccac aacgcttcgc
     6121 ccgcaggcca gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcaaataag
     6181 gcagccagcg cgagcggaac accggacggg atgatgacat tacggttaat tcctgaactg
     6241 tgccggatgc gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc
     6301 atccgacatt acactgatta agacttcgtc gatttctcaa aacggcgcag caactgattt
     6361 ccacgctcaa cggcagtatc cagcgcctgc tgtggcgtct tcttaccggt ccacacgctc
     6421 tccagctctt catccacaat cacgcggatc tgcggcatat tgcccagacg caaccctttg
     6481 gtgaacggca acggcggctt gttcagcatc tgacgcgtcg cggtatccgc ccctgggttt
     6541 ttctcataaa agccctgctc acgggtcagg tcatacgccg ctttggtgat tggcagataa
     6601 ccagttttct gatgccactc ggcagcgttt tctggcttcg cgaggaagtc gaggaacttc
     6661 gccacacccg tataagtttc tttatcttta ccctgcatta cccacaggct ggctccaccg
     6721 ataatggcat tttgcggtgc atctttcgca tcggcgtcgt aaggcatcat gcctacgccg
     6781 tagttaaatt tggcgtactc gcgaatgttg gcaagagaac cggaagaggc agtggtcatc
     6841 gcgcaatcac cgttatagaa cttctcagtg gattcatcct tacgcccgac gtagctgaaa
     6901 tcgcccttct tgttcatctc ttcgagcata gtgatgtgtt tcacctgctc cggcttgttg
     6961 aactccagca ccgcgtctgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg
     7021 ttccaggcgc taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac
     7081 ttcataccgg aggctttcag tttcgcggca tagtccgcca gatcctgcca ggttttcggc
     7141 ggctgttccg ggtctaaacc ggctttcttg aaggcgtctt tgttgtaata gagaacggga
     7201 gtcgagctgt tgaacggctg ggagagtaag tggccggttt tgctgtcgga gtagtaacct
     7261 gaaaccgtcg gcacaaactg cgactcatcg aactgaatcc ccgcctcttt aaacacgtca
     7321 tatactggtt taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc
     7381 aaaatagcag gcgcgttgcc ggtacgaaat gcggcaatcc ccgcgcttaa attctgttcg
     7441 tagttgcctt tataggtcgg tacaattttg taatccgggt tttcggcgtt aaaacgttgg
     7501 gccagagaat ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc
     7561 gtcactgcct gtgcattccc cattaacgcc agtccgagcg ccagtgctga agctgtataa
     7621 tgtaacggtt tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta
     7681 tgctcgcggg gtaacatgac atgctcgaat tacagaaaaa taactttttt gttacatttg
     7741 taagatagta aggtgtcaga aagatgacaa ggcggtgacg gtacgggtga gggaaaatgg
     7801 gagatggggc acggataagc gggaaaaata gaaggtctga atcaaactct acagattgct
     7861 catcgtttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt
     7921 caatatattg caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttacat
     7981 ttgtaacctg tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc
     8041 taaatacgca cttctcaccg cttcattcgc cagcagcgcg tcgccggtat cggagagcac
     8101 tacatgaccg ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt
     8161 ctgctcgacg aggaagatgg tcatcccctg ctcacgcagc tgctcgatgg tgtcgaaaat
     8221 ttgctggatg ataatcggcg caagaccgag cgatggctca tcaagcaaca ataaacgcgg
     8281 gttgctcatc agcgcgcgac caatcgccag catctgctgc tcaccgccgg acatggtgcc
     8341 cgcccgctga acacggcgtt catggagacg cggaaacagt tcatacaccc actttatgcg
     8401 ttcctggaac tggtcacgtt cagcaaaaaa accgcccatc gccaggttct cttccaccgt
     8461 catccgcgag aaaacacgac gcccttccgg gacaatcgcc accgcttcgc gcatgatttt
     8521 cgccgtctgc cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg
     8581 cgggtcgccg cataatgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaataag
     8641 ggtgacaatc tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc
     8701 gtagtgggcg ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta
     8761 aataggcacg gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg
     8821 gcgtcccctg attgaccacg taaattcggt ccgaaattcc catcaccagc ttcatatcgt
     8881 gttcaattaa caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca
     8941 gctctttcgt ctctttcggg ttaagacctg ccgcaggttc gtcgagcatt aaaatctccg
     9001 gctgcgtcac catgcagcgg gcaatctcca gacgacgctg gtcaccatag gccaggttac
     9061 tcgcctgacg gttggcatgt tccagcaaac caatgcgctc aagccaggta gcggcgcggt
     9121 cgagcgcttc gctctgggcg cgacggaaag aaggcgtttt caaaaggccg gagaacagcc
     9181 cggttttcag ttgctgatgc tgtgccacca gcaggttttc aattaccgtc atttcacgga
     9241 acagacgcac atgctggaag gtacgcacca cgcccatgcg ggcaatttgc tgccccggta
     9301 aaccttccag atgctgatcg cgcagtaaaa tagtgccgcc ggtgggttta tagaatccag
     9361 tcaggcagtt gaagaccgtg gtttttccgg caccgttagg tccgattaac gagacgatct
     9421 cctgcgggta cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca
     9481 tcaggccgtt aacggataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt
     9541 tttcagcttc agttgcgggc gcgtcatggg cagcaagccc tgcggacgcc agatcatcat
     9601 cagcaccatc aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc
     9661 gcgcgacacc accagcaaaa ttgccgccag aattaccgca aattgcgagc ccataccgcc
     9721 aagcaccact atcgccagca caaacgccga ttcggcaaag gtgaaggatt ccgggctgac
     9781 aaagccctga cgcgcagcaa acagcgttcc ggcaaaaccg gcaaacgcag cgctaatggt
     9841 aaaggcagtc agcttgatac gacgcgggct taagcccagc gaacggcagg cgatctcgtc
     9901 ttcacgcaac gcttcccacg cacggcccag cggcatccgc agcaggcggt taatgacaaa
     9961 caggcttaat accaccagca gcaacgccac caggtacagg aagataacgc gatcggaggg
    10021 atcgtatttc aggccaaaga aattactgaa cgtgtcccag ccgccttcgc gggcggtacg
    10081 gctgaactcg aggccgaaga aagtcggttt cgggatctga ctaattccgt tcgggccacc
    10141 agtgatttca gtgttattga gcagcaatat gcgcacaatt tcgccgaaac cgagggtaac
    10201 gatcgccaga tagtcaccgc gcagacgcag caccggaaag ccgagcagga agcccgccgc
    10261 cgccgccatt aacccggcaa tcggcagaca ggtccagaag cccagaccgt aatagtgatt
    10321 gagcagcgca aaagtgtaag cgccgatggc gtaaaagccg ccgtacccca gcaccagcag
    10381 accagaaaga cctaccacca cgttcagccc gaggccaagg ataatgtaga tcatggtcag
    10441 ggtggcaata tccaccgtcc cgcgtgaaac cataaacggc cacgccaccg caagcaccaa
    10501 cagcgccacg aggaacagtt tctgtttcac cgtggagcca tcaatcgcgg gcagaataaa
    10561 cttcggtccg gaaacgcttt tcagcccttt ctggaaagcc ggtcgcaaaa gctggaagaa
    10621 aaagaccacc gccgtgccga taaacaccca ctgccaacgg atgtctgaag ccgtgtcgac
    10681 caccagtttg gtgccatcca gctccagttg cacgcccata aagacgccag cgaggacaaa
    10741 gaacatcgcg gcagagagca acgccattgc aatatgcatc ggtttcatac tttctctacc
    10801 tccgggcgac ccagaatacc ggtcggcatc accagcaaca caagaatcag cagggcgaat
    10861 gagaccacat ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc
    10921 aggatcagcc cgccaatcat cgctcccgga atgctgccaa tcccaccgag caccgcagcg
    10981 gtaaaggctt tcatcccggc cataaagccg atgtaggggt taatgacgcc gtagaactga
    11041 ccgagtagca cacccgccac cgccgccatc gccgcgccaa tcacaaaggt cagcgcaatc
    11101 acccgatcgg tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacgg
    11161 caggcgcgcc ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaaa
    11221 gtaacaatcc atatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg
    11281 cccaccaccc actgaccgtt aaacagactg ggcagcgcca cgtcgcgcga accttcggtc
    11341 agactgacat agttttgcag gaagatggac atgccgattg cggaaatgag cgcaatcagg
    11401 cgcttagagt tacgcaccgg gcgataggcc acccgttcga tactccagcc gtaggcgctg
    11461 gcaatgacga ttgcgccgac gaatcccgcc gccaccagta gccagccggt atcaatgccc
    11521 agcatcatca gtgcggcgat gatcataaag gagacatagc taccaatcat ataaacctcg
    11581 ccgtgggcga agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc
    11641 agcgcgtagg tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac
    11701 tgctcagaca taaggtaacc tttctaaacc cgcccgtttt tcacgggcgg tgggatgatc
    11761 acttggctgc cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct
    11821 taagatcgcc tttttcatcc cagttcagcg gcccaatcac ggtgttcgca ccgttagctt
    11881 ttaaatcttt caccagtgcc agcggctcat cgctgccggt acgctcaagg gcagtcgcca
    11941 gagattgcac cgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg
    12001 ctttcaacgc atcaacaatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag
    12061 tgaccaacat gccttcggca gcatcgccag caatattcga caacgacgca ttacccacac
    12121 cttccggccc cataaactga gttttcaggc caacggaacg ggcctggcgc agcatctgcc
    12181 ccatttccgg gtagtaaccg ccgtagtaaa cgaagtcgat gttttctttt ttcaggcggg
    12241 cgatcagcgc ggagaaatct ttctccccgg cggtaatacc gtcgaagaaa acgacgttgg
    12301 cattggctgc tttcaatccg tcctgcaccg aacgcgccag cccttcacca tactgttgtt
    12361 tgtcgtgaat gatcgcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg
    12421 ttggcccctg ggaagagtcc agcccggcag tgcgcatgat gtgttgataa ccgcgttggg
    12481 tcagctccgg gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat
    12541 ctgaagcagg ctgggtagag gaggaacaca gatggccgat gacgtattta atgccgtcgt
    12601 taacgatttt gttggcgacc gcaacggctt gtttcggatc gcaggcgtcg tcatattcca
    12661 cgcccaccag cttatcgccc ttaattcccc ctttggcatt aatgtcttta atggcctgac
    12721 gcgcgccgtt aaattccata tcgccccact gagcaatcgg accggacatc gcgccgacaa
    12781 cggcaacttt aatatcgtcg gctaaagcgg tgtgtgaaat tgccagtgca atcatccctg
    12841 cgatgatagt tttcgcattc cgtttcatag tcaaaaatcc ccactcgtaa tgttgtgttg
    12901 ctttgttttt atgtgttaac aaatcaggct gttctttttt tatactgcgc tgtttttacc
    12961 tgtctgatta agggtttagc gcagtatttt gtgataatag cgattaaaat ccctattttt
    13021 cagtcgatta agaacagata atattctgaa ttaattgata gataaacaga aaaaagcacc
    13081 gttgtcagca taaaataacg gaaca
//
LOCUS       DAYPHN010000141        12819 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000141 DAYPHN010000000
VERSION     DAYPHN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.141"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(109..1596)
                     /gene="yeeO"
                     /locus_tag="ACG0BW_003703"
     CDS             complement(109..1596)
                     /gene="yeeO"
                     /locus_tag="ACG0BW_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFK7260621.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1757..1832
                     /locus_tag="ACG0BW_003704"
     tRNA            1757..1832
                     /locus_tag="ACG0BW_003704"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1790..1792,aa:Asn,seq:gtt)
     gene            complement(1870..2820)
                     /gene="cbl"
                     /locus_tag="ACG0BW_003705"
     CDS             complement(1870..2820)
                     /gene="cbl"
                     /locus_tag="ACG0BW_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFK7260622.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2922..3839)
                     /gene="nac"
                     /locus_tag="ACG0BW_003706"
     CDS             complement(2922..3839)
                     /gene="nac"
                     /locus_tag="ACG0BW_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFK7260623.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4165..4240
                     /locus_tag="ACG0BW_003707"
     tRNA            4165..4240
                     /locus_tag="ACG0BW_003707"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4198..4200,aa:Asn,seq:gtt)
     gene            complement(4296..5231)
                     /gene="ldtA"
                     /locus_tag="ACG0BW_003708"
     CDS             complement(4296..5231)
                     /gene="ldtA"
                     /locus_tag="ACG0BW_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFK7260624.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5293..6372)
                     /gene="cobT"
                     /locus_tag="ACG0BW_003709"
     CDS             complement(5293..6372)
                     /gene="cobT"
                     /locus_tag="ACG0BW_003709"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFK7260625.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6384..7127)
                     /gene="cobS"
                     /locus_tag="ACG0BW_003710"
     CDS             complement(6384..7127)
                     /gene="cobS"
                     /locus_tag="ACG0BW_003710"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFK7260626.1"
                     /translation="MSKLFWAMLSFITRLPVLRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTSDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7124..7669)
                     /gene="cobU"
                     /locus_tag="ACG0BW_003711"
     CDS             complement(7124..7669)
                     /gene="cobU"
                     /locus_tag="ACG0BW_003711"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFK7260627.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIYADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7803..7994)
                     /locus_tag="ACG0BW_003712"
                     /pseudo
     CDS             complement(7803..7994)
                     /locus_tag="ACG0BW_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7973..8161)
                     /locus_tag="ACG0BW_003713"
                     /pseudo
     CDS             complement(7973..8161)
                     /locus_tag="ACG0BW_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9158..10276)
                     /locus_tag="ACG0BW_003714"
                     /pseudo
     CDS             complement(9158..10276)
                     /locus_tag="ACG0BW_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10342..11193
                     /locus_tag="ACG0BW_003715"
                     /pseudo
     CDS             10342..11193
                     /locus_tag="ACG0BW_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(11233..11525)
                     /locus_tag="ACG0BW_003716"
                     /pseudo
     CDS             complement(11233..11525)
                     /locus_tag="ACG0BW_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(11605..12819)
                     /locus_tag="ACG0BW_003717"
                     /pseudo
     CDS             complement(11605..12819)
                     /locus_tag="ACG0BW_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         3376 a         3440 c         2852 g         3151 t
ORIGIN      
        1 acagaggaat cgtgagaacg aggcgaatat tagcgatgcc cacccacaat gtcaaagcct
       61 gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgttgttt attccgacac
      121 aactggcttt ttttcacact tttgcggttc gggtcgaggg tatttccata gccaacgtcc
      181 agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa acattcccat
      241 ccagacacca accacacccc agccaagcat gatgcccagc acataaccga cgacaacccg
      301 gcaaccccac atgctcaaca tagaaaccca catagcgtaa cgggcgtcac gagccccctt
      361 aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag catttagcca
      421 aatcagaatc acaacgacat gtttaacctg tggatcctgg gtgtaaaacg atgccataac
      481 cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg acagccagaa
      541 gacatgccgc aactgaatct ctgcttgcgc tatctgccct acccctaacc ttcggcctgt
      601 aatgatcgta gaagcagagc cgagcgcact tccgggtaag ttgataagag ccgcaattga
      661 aaacgcgata aaatttccgg caataacact ggtccccatc ccggcaacga acatttgggt
      721 taataaccga ccactggtaa ataacactga ttcgacactc gctggaatac caatccccat
      781 gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg aaatccttag
      841 cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa tataacgaga
      901 aatggttaaa cccaacccag ccccgacaaa tcccagtccc ggccaggaga aaaggccgta
      961 aatcaatatg ccgctaataa taatattaag aatattcagg ctaccgttaa tcagtagcgg
     1021 tattttcgta ttccctgcac cacgaagtgc cccgctacca atcaaagtga tggcagctgc
     1081 tggataactg agtaccgtca gctccagata agtcaacgcc agtgctttaa cttctgtcgt
     1141 ggcatcaccc gcgacgaaat caataatttg ctcgccaaaa tgatgaataa gcgttgccaa
     1201 cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg
     1261 tcgatcccgc ttaccgagac taaatgccac aacgacagta gtaccaagat cgatagcagc
     1321 aaaaaaagcc ataatgacca tattgaagct gtccgccaat cccacgccgg ccatcgcatc
     1381 ttttcccagc cagctgacca gaaaagtgct cagaaccccc atcaacagga cacaggcatt
     1441 ctccatgaag ataggaacag caagcggggt tatctcgcgc cagaacaaca ctttgtagct
     1501 cttgcgttta gcgtgccagc gagtgccgtg aacaacctgg cgtaaagcag aggagatatt
     1561 caaagccgac cttaattgca gaaagtgaaa ccacatttca aataatgagg gagaatcagc
     1621 aaagctgcaa agattttcgc caacaaattg tctacaaatg caacaaactg ttgatagaaa
     1681 cggcaaacag ttggggaatt taaaaatcgg gtttgacaaa agatttttcg ccgttaagat
     1741 gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta
     1801 tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc ttttatagcg
     1861 ggccttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca cctggcgctt
     1921 gatatcctca actgacagtc ctgcattaca aagttccaga aagcgccaga catagttacg
     1981 ctgaagttgt cctcgcttga gtcccaacca gacagtatta gcatcgaaaa gatgccgcgt
     2041 atccaggcgg attaaattct tttcctcttg ttcaccgctg gattgttcgg caactaatcc
     2101 gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgcg cacttaatac
     2161 aatatctgcc agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc gccccgtaat
     2221 cccctgtcgg taagtgatta acggccactt cgctattgat tccagcgtca atggtgtaat
     2281 ttgcgtcaag ggatgatcgt gtggaacaag caaactatgg tgccaacgaa accacgggaa
     2341 ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat cagcttcgcc
     2401 attttgcaac aacgtcgcaa tttcctgtgg cgtaccctgg attagctcca gccgaacctc
     2461 cgggaaaagt tcgcgaaaag ctttaatgac ctcaggcaag ctataacgtg cctgagtatg
     2521 cgttgttgca atagtgagaa cgccagacgt atcgttggta aacaggtctg caagccgacg
     2581 aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt tgcccggttc
     2641 agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc
     2701 cagttcccga atatgacggc tgacgccgga ctgtgaggta aaaagcatat tcgcaacctc
     2761 tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt ggaaattcac
     2821 ggtaaactcc gggcagttca gattttccgt tattgttaaa gtctaatgcc cggcataaca
     2881 aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac caattgccac
     2941 tgcctttttt ccatcactgg agaactcatc actgacatta acaactcttt cactgcctgt
     3001 gcctgtggtg ataagttcgc tctggcgggt aaatttaatg acaaagagag actcatggaa
     3061 ggtgtggtaa tgcgtgacat ccacccgttt actgcgccac ataacgaacg cgcggccgat
     3121 tcgggtaata ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt ggcaatagac
     3181 tcaatttcac caatgacttt tgccgtgagt cgccgtaggg aaaaagcctc atcaacacga
     3241 agtctaacag cactgtaatc actgggaaga aagaggttca tttgcgcaat agcattaaca
     3301 tcaacgcttt gccccggaca atcttgagtc cctaccagga aaagatcttc tttcagtaaa
     3361 gcctgactgg atacaccagc cacaggggaa tgctcataaa tcaccgccat atcgagttgg
     3421 tgatttatca gtttttcgtt aagcactgca ccactatttt catgaagata gataacgacc
     3481 tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg tgatggatga cgcagcggtt
     3541 cctggcgcaa agccaatcga gacttgcccc gataatgact gaccaacgtt atgcaccgcc
     3601 agttgggcct gctcacactg acgtaaaatg gcccgcgcat gggtatagag aatttttccg
     3661 gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa gttgttgatt taactcacct
     3721 tccagtgtgg caacctgctg gctgagcgct ggttgtgcga tatgcaatac ttcagcagcc
     3781 tgagtcaggc taccaatatc tacaattttt acgaagtatt tcaggcgtct gaagttcatg
     3841 ttgcctccgg tttttgagaa tcggcccaag tgccgccatt acttacaacc agattgcaag
     3901 atgcttgcca gttttatttt ggcgttgatg tacaagctaa ccaactgtca aataagagat
     3961 tatgatagat tcgtcattgg ctcttttcac cagcagtcgc gttcccctgc cctataaaag
     4021 gagggtatgc accacgatgg ttcattacca ataagattga aatctcatca ctttgttgaa
     4081 attgacagca aacaaacaaa aaaatgcatt tcaccctttg acatcaccat gcactgccat
     4141 taatatgcgc cccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt
     4201 aatccgtatg tcactggttc gagtccagtc agaggagcca aattcaaaaa agcctgcttt
     4261 ctagcaggct ttttgctttc taattaccaa cgctcttaaa acatctgtct tgaaccagaa
     4321 ctaatttgca caggcattcc cgatcgacgt tgcaacgcag catttgcgcg attcacatca
     4381 acttcttgcc cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg cacttttcgg
     4441 tcagactcat attctgcacg attgcgcgac agcggctcat gaacttccag ccagttcgag
     4501 ccatctggtt cagtggtgta ttttactggc tggtcgataa tttgcacacg cgttccaaca
     4561 ggaacattat caaacagata tttgatatcg tcattgcgca gacgaataca gccctgactt
     4621 acccgaagtc caataccaaa attggcattg gtaccgtgga tggcatacaa cctgccaata
     4681 taaatcgcgt acagccccat gggattatcg ggccccgcag gaacaaatgc gggcaaactc
     4741 tcccctcgtt tcgcatattc gcgccgagtg ttcggcgttg gcgtccaggt tggagcttct
     4801 tgtttacgtt caacggtagt cacccagtta cgcggggttt ctcgcccagc ctggccgata
     4861 ccaataggaa agacttccac agtattactg tctggcgggt agtaataaag acgcatctca
     4921 gcgacgttaa ccacaatccc tttacgaaca gtggcgggca aaatcagttg ctgcggaatg
     4981 gtgagttgcg agccagactt cggcaagaaa acatcagcgc ccggattcgc ttccagcatg
     5041 ttacttaacc cttgcccgta ttgtgcggca aaagtctcca gcggctgggt attgtgatca
     5101 ggaacagtta cagtaagcga ctgccccacc aaacggctac cctctggagg taatggataa
     5161 gttaccgcca ggctagtatg gctggcaaaa agcagagcaa atgagcaaag aatatttaca
     5221 cgacgcatca tgtccctttc ctatgtcgcg aaagctatcc gttaagtata gcttttatca
     5281 gacttttcgt ttttaactgt tcaaatcaga agtcgtattc cccggtagaa caatattact
     5341 ggcagcaagt tcgcccatgt tgttatatat cgcacaggca gcttcgatga tgggcatcgc
     5401 cagagctgcg ccactcccct cacctaaacg catctccata ttgagataag gctccagccc
     5461 caaatgcgac agcgctatac gcgccccttt ttctgccgac aggtgagaag gaatgagata
     5521 cggtttgatt gcaggagaca tctggcaggc tgcgagcgca gcagcataag aaagaaatcc
     5581 atccagcaac acgggtaaac cgcaggaagc agcgcctaac atcacgccag ccattccgac
     5641 caaatcaaat ccccccactt ttgccagaac atcaacgcca tcctgcggat ttggttgatt
     5701 caacgtaatc gcccggcgca caacatcaat tttattagcc agtttatctg tcggcaggtt
     5761 tgcgccaatc ccaaccactt cttcaggatc ccggccagtg attgtgctga ctattgccgc
     5821 tgccggtgtc gtgtttgcca tccccagttc acctacacca aacagcgtga caccgttttt
     5881 tgccagctcc cgcgtataac atatgacgtc caaaagcaac ttttcagcct gacggcgact
     5941 cattgccgga gctgaagcaa tattgccgct acctcgtgcg acacgcatgt tgataagccc
     6001 ggggataggc tcagcactat caataccaac atcaactacg tggacgttag cgcccgcttg
     6061 tgctgccagc acacacacgc cggttgttcc gcgggtcata ttttcagcct gaatggctgt
     6121 cacttctttt ggggaaatag cgaccccttc ctcccagacg ccgtgatcgg cacacataac
     6181 cagtaccgct tttttgccca catgcggtat gccattcaac cccggcattc ccgccagttg
     6241 tatggcaagc gcctccagcc ttcccaggct accaacaggt ttgagtaacc cgtcaatatg
     6301 ccgttgtgca cgcgacatag cggcaggatc gatagcaggg atcgtattca gtaaatcggc
     6361 aagtgtttgc atgtcacgtc tctttatagc agtgccagca ggaagaccag ttcaccaagc
     6421 tctatagcag ctcccagcgt atcgccggtt tgcccgccca gcgtacgttt gagaagctga
     6481 ccgaggatga aaatcgccac catcgtcacc accatagcag ccacaccatg catacccggc
     6541 aataatacgg cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg tcgcccatca
     6601 attttgccaa tgaacacatt gccaagccct tcttcccggg cataacgatg acgatacatc
     6661 aataatgcgg cagtcccgcg gctgaccgca catgctgccg ccagcgatgc aaggatcggt
     6721 tcgccacgca gagccaactc gcttaatacc agaatctttg ccagtacaac aaaaatcaat
     6781 gccagaccgc catgggtgcc taaacggcta tcacgcatga tttccaacat gcgatcgcgg
     6841 ctacgtgcag aaaatacgcc atcactggta tcggcgaggc cgtctaggtg gaatcccccg
     6901 gtcatcagcg ccagcacaag tacgctaaac agtgccgcca gtggtacgcc acaccatgcc
     6961 tgcagcacca tgaagaccag cccgctaatc gcgccaagca ataatccaat taaaggaaaa
     7021 gtaataatac cgcgagaata atgctcgaaa tccagtccct gggaccagcg acgcagtaca
     7081 ggcagacgcg taataaatga gagcatcgcc caaaataatt tactcatttg atttttactc
     7141 caatacccga aaccaccagc catacttcat ttgccgcagc ggccagctgc tgatttaccc
     7201 gcccggcaat atcacgaaaa tgtcgtgcca gacgactctc cggcacaatc cccattccca
     7261 cttcgttagt cactaataca acctttgcgg ggcaacgttg gcaggcagca atcaacgact
     7321 gaatctcagc attaatcgcc tgttccatcg cctgataatc ccattcatca gggtctttat
     7381 cgccgccata atcaaacaac agattagtca ccattgtggt aacgcattcc agcaacacag
     7441 cttcatgcgg gttaatgtct gcataaatta attcatcaag atgttgccaa cgctccaccg
     7501 tgcgccagtg cgccggacgg ccttgccgat gatgttctat ccgtgcagcc atctcgtcat
     7561 caaggatttg cgaggtggcg atatacagaa cctgtgaaga gtccccaata agcgcctctg
     7621 cgtggcgact cttcccgctc cgtgcgcccc ccgtcaccaa aatcatcata ccgactcctg
     7681 attcaattgt atgatggtat tcattgaaca gaacaatgga tcccccacca ttgctgaccg
     7741 caaaacattt acacgatcaa tactgcgcgt aatataatta aaaatccagc attctcaatc
     7801 cattacgaaa gaagatggca ttgtggaata gccagacttc ctcgtgttaa cgtccgacaa
     7861 aatgcgtcat tgacagcggt ttctgtggat tgacacattt cattcagata atgaattaat
     7921 gctactgccg gaccaaccag caaacgggca ttgtgttctg aaatccaggc tattgattca
     7981 acgtcagcga cagatctgcg ctgtaaaaga acttgtaaaa caacgcaata gaattatccg
     8041 gaatggtggc gactatgcac tagaacacca caatttcgct ctctcggtaa ggcactgtca
     8101 cagcattcaa cagaatgtga cttgccagat ttattagcgc caccagatgt ttaaccgggt
     8161 aaccacgacc cagtcgggga tatattccat cttctcgctg acatcatcaa gaataaaaag
     8221 gtttatcaca ctcagagcaa cgttcttcag caggcgggat aattcttcaa catttaaaag
     8281 atgtgttatt aaaggcatac aaactggacg tattgtattt tcttttggtg gttgacctaa
     8341 ggtagcagtt tatcctgatg cgctgagatt tctgtaatat ctacgtcaac attctcctcg
     8401 aatagaaaat gcagcctcgg caagtgattc acatttttta acaaaacgtt gttagcgtgc
     8461 cattttcaga agctctccaa gaaagcgaat tcttttctca aattctgcat tctcagacac
     8521 agctttctac tccatggcaa tggcccaaat acgaagttgc tcaggatcat tagtggtgct
     8581 gagaagttaa ataaccatgc ggagtaccac tcgtcatcac gtatttatat atcatgaagc
     8641 tattgttgtg ttttgtacat cagtagatgt attctgatat actccttttg ctagacataa
     8701 cctttcacct gcttgcaaag cttctgtgtt ctgacattgc caaattgttg caattctgta
     8761 tccagccttc tttcagtcat agcttcgggc cgcgataaga ctcactgatc tgaccctgat
     8821 tcctcttgca gactttatag accaattaaa atgcagtttc tgcaggtcaa cgtctgacca
     8881 tcattgtcat cactctggcc attagagtaa ccttctgcat tcatcctttt gtaaaaagtt
     8941 tatgtcagta tcagcaatta accggacctg atactgatat gagtcttacc gcatatacgg
     9001 tcaatttcag caattaatta cattatccac gccaaagtgt ttgtcatcgc aatgatggta
     9061 ccttctttca gacaccattt tttcaactcc gttttccacg gaccgcactc ttatgtcaag
     9121 agtgcggtga tcctacccac gtaatatgga cacaggccta agcgaggttc tggttttcaa
     9181 attgttccgg actgaggccg ccacaccaac tgtgccgccg ccaccgattg taatcacatt
     9241 cgatataatt aaacaccgtt gcccgcatta tttcccggct gataaagtgt tctccatgga
     9301 tacattccac tttcagcgaa tgaaagaagc tttccacgca gattatgccg cttcagttgc
     9361 gcctgataat ctgctgaaca gtactggcct ccacggtccg tgtgaacgat aacgttccgg
     9421 ggcctcttac gccgccatag cgccatctgc agggcatcgc aggccagttg cgccgtcatg
     9481 cgtggcgaca ttgaccagcc aataacggca cgtgaccaca ggtcaatgac cactgccaga
     9541 tacagccagc cttcatctgt acgtaagtac gtgatgtctc ctgcccactt ctggttcggg
     9601 ccactggcgt aaaaatcctg ctccaacaga ttttctgaca caggcaggcc gtgtgcgcgg
     9661 tagctgaccg ggctgaactt ccgggaggcc tttgccctca gtccctgacg gcgcaggctt
     9721 gccgccacgg tttttacgtt aaaggggtaa ccctgagcac gcagttcatc cgtcaggcgt
     9781 ggggcaccgt aacgctgttt tgaccgggta aaagccgcga ggacaacgct gtcgcagtgt
     9841 tggcggaact gctgacgcgt gcttatcctt gtccgccgct gacaccacgt ataccagccg
     9901 ctgcgggcca cccggagcac gcggcacatt gctttgatgc tgaactcagc ctgatgtttt
     9961 tcaataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc ttttggagga
    10021 tagccagctc ttcatcccgt tctgccagct ggcgtttgag acgtgcaatc tcggtagaca
    10081 tctccagttc acgttcagaa gacgtctgct gattttgctg tttactgcgc cagttgtaga
    10141 gctgtgattc atacaggctg agttcacggg ctgcggcagt aacaccgatg cgttcagcaa
    10201 gcttcagggc ttcactgcga aattcaggcg aatgctgttt acggggtttt ttactggttg
    10261 atactgtttt tgtcatgtga gtcacctctg actgagagtt tactcactta gccgcgtgtc
    10321 cactattgct gggtaagatc accttattgc tgcgctgata atatggaccg caaacctatt
    10381 gaagatgtga tcttcgaaat taataacttt atctcgctcg gtggtcgcac cattgttgat
    10441 gccacagggt ctgagtctat cggacgagat gcgcaggcat tacgagaagt cgcattaaaa
    10501 acaggtctga atattgttgc ttcctccggt ccctacctgg aaaaatttga aagccagaga
    10561 attcataaaa cggttgatga actggcggca acgattgata aagaattgaa tcaagggatt
    10621 ggcgatacgg atattcgtgc cggaatgatc ggtgaaatag gtgtctcacc gacatttacc
    10681 gaagccgagc ataacagctt gcgggctgcc tcgctggcac agattaacaa tcctcatgtg
    10741 gcgatgaata ttcacatgcc gggctggctt cgtcgcggtg atgaagtact cgacattgtg
    10801 ttaggtgaaa tgggcgtctc gccaaataaa gtctctctcg cacactcgga tccgtcagga
    10861 aaagacgtgg cgtatcagcg gaaaatgctt gataagggtg tctggctgga attcgacatg
    10921 attggcctag acattacctt cccgaaagag ggaatagcgc ctggggtgca ggagactgcc
    10981 gatgccgtcg ctcatctcat tgagttggga tacgccgatc agcttgttct cagccacgat
    11041 gtcttcctta aacaaatgtg ggctaaaaat ggcggtaatg gctggggatt tgttccagat
    11101 gtttttctgg cctatctggc ggagcgcggc gtcgataaaa cgatcctcaa aaaactctgt
    11161 atcgataatc ccggacgatt attgaccgca taaaaccatt atcccccggc acactgcgcc
    11221 gggggatgtc aattaatacc ccgccgttaa atcatccacc aagcgcgagt cggatgcgcc
    11281 gtacaactca ccatccgacc caaccataat gctttgcgtg ctgcccatcg cctcattcag
    11341 cgccactttc tgaccttttg catccagcag cttgagcgta tccggactaa acccttttca
    11401 acacgcagct cgtccggcaa ccactgatag tggaaacgag gcgcattggt cgcttcggca
    11461 acattcattc cacaattgat gctgttcacc accatttgca gcacggtagt gatgatccag
    11521 ctaccggtaa caaagtgagt acataaaggc catactgtca cgaacagtgc aaaagtggct
    11581 cttccgcaac ctgggtgaag attttcatgc cagtggactc attaccctcc gcctaagtag
    11641 ttcaagtccg gctcgaccat acatctggcg tatcagcacc ttcaggcgat ttacatgacc
    11701 ttcgacaaca ccagtactcc agcgactgct gattgcctca catatagccg cagcatcagc
    11761 ttccatcccc gcagctacgc accgaagttc tgctgagccg ctttcgtgga cccgagtgaa
    11821 ccagctgctt agctgtgatt tattttaggt tttcagaata cgatagaact cgagtaccag
    11881 ttgctgcgct attttcaact ccggttcttt ttcacacatc agactaataa atcggaaagc
    11941 ataattttct tcacccctga ttattctcca gggcatcaac cagcggctta cccgggacac
    12001 tgaaggaagt cttgcggcag tagtaaccgg tgggatccag cctttacgcc atttggcaac
    12061 agcatccctg acgatggttt cactgcctgt aacccccctg gccaccattt cccgccatat
    12121 ccggccggag ttataattac cacattctcg ttgctctttt aaccactccc tccatgggtc
    12181 cagaagccct cgctttggag gcctagttga catttcaggg aatgttcctg aacccaccca
    12241 gcgactgact gtcactcgcg acaggcctgt aatacgggat atttccctga aactacatcc
    12301 cttgttatgc agggccataa cctctgtcca acgctgatgc cgttttttgc ggatgtgctg
    12361 tttaaggcgt tccagacgac ggagcgatgc tacaggcaca gatatttctg gctcaggtga
    12421 tttcttcagt gataactctc tgacaacaag acgtatcaga ggcatatgtc tgtacatcat
    12481 tcgttcaggc tcatcaccaa tattttttag caggtgccat cgatcggcca cctgtctggc
    12541 ctgaggtgca ccttcacgtg ctgctgtcgc atagactcca ctgcgatcac gcgagacaac
    12601 ctgtatttcc ggatattttc tgaaccaggt cgccagcgta cgctgatcac ggccgggaag
    12661 caggacgagg ggacggtgag tatcaagatt gactattaac ataccgcagc agtggccccg
    12721 atgccacgcc cactcatcaa taccgacatg aggcgcgcct gactgtttcg tctccggggt
    12781 attgataacc ctgcgaagaa gagtatcagc actgcaacg
//
LOCUS       DAYPHN010000142        12442 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000142 DAYPHN010000000
VERSION     DAYPHN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.142"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            41..568
                     /locus_tag="ACG0BW_003718"
     CDS             41..568
                     /locus_tag="ACG0BW_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFK7260628.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            577..2676
                     /locus_tag="ACG0BW_003719"
     CDS             577..2676
                     /locus_tag="ACG0BW_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFK7260629.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVSTPVKKQKWK
                     KTVTDDVNPWLTSGGWL"
     gene            2673..2885
                     /locus_tag="ACG0BW_003720"
     CDS             2673..2885
                     /locus_tag="ACG0BW_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260630.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2813..4360
                     /locus_tag="ACG0BW_003721"
     CDS             2813..4360
                     /locus_tag="ACG0BW_003721"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFK7260631.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYETDGNDSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQ"
     gene            4338..6365
                     /locus_tag="ACG0BW_003722"
     CDS             4338..6365
                     /locus_tag="ACG0BW_003722"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFK7260632.1"
                     /translation="MPQRNDSSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6452..6775
                     /locus_tag="ACG0BW_003723"
     CDS             6452..6775
                     /locus_tag="ACG0BW_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFK7260633.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6768..7043
                     /locus_tag="ACG0BW_003724"
     CDS             6768..7043
                     /locus_tag="ACG0BW_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFK7260634.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDADMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFSGKPMIFIE"
     gene            7055..7633
                     /locus_tag="ACG0BW_003725"
     CDS             7055..7633
                     /locus_tag="ACG0BW_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260635.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKVPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7630..8031
                     /locus_tag="ACG0BW_003726"
     CDS             7630..8031
                     /locus_tag="ACG0BW_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFK7260636.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIREQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8043..8786
                     /locus_tag="ACG0BW_003727"
     CDS             8043..8786
                     /locus_tag="ACG0BW_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFK7260637.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDSDFVAVATVAVSAAG"
     gene            8847..9233
                     /locus_tag="ACG0BW_003728"
     CDS             8847..9233
                     /locus_tag="ACG0BW_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFK7260638.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGVIENAGDLEHEVLTPEKS"
     gene            9242..9571
                     /locus_tag="ACG0BW_003729"
     CDS             9242..9571
                     /locus_tag="ACG0BW_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFK7260639.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9543..>12442
                     /locus_tag="ACG0BW_003730"
     CDS             9543..>12442
                     /locus_tag="ACG0BW_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFK7260640.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQTRNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMA"
BASE COUNT         3046 a         2896 c         3758 g         2742 t
ORIGIN      
        1 ataaataaac ctgattttca cctgccagat ggagttgctt atgtcaaatg tgagcgggat
       61 cggtgatgct tattactgga gtgtttttaa aatcgccgag gcctttgggc ttcaccggga
      121 cacagtaaaa aaacggctcc tcgcggccaa cactcctgtg gctgcgactg tcagggggaa
      181 ccccgtttac gccctgcagc atgtcgggcc cgccctgttt agtgtgaagc atgaggcagc
      241 agactctgtt catgatccat cccgtatgga gccgaaagag agaaaagact ggtaccagtc
      301 tgaaaatgaa aggatcaagc tggaaaagga gcagcggaaa ctcatcccag ttgatgaagt
      361 agtcatcgtc tattcgtcca tgagaaaggc tgtcgtccag gttctggaga caattccgga
      421 tgttcttgaa cgcgattgcg ccctgactcc tcaggccgtc ggcgttgtac agcaggccat
      481 tgatgacctg cgatacactc ttcaggaaaa atcctacgag gcttgtgctg ctgaattaat
      541 tcctgatgag gaaggagaga gtctctagga ggaataatgg gtttttcatc agcccgaaat
      601 ttgggaaggg acatatcggc aggattttcc ccaccacgtc gcatgccgat ttcggaggct
      661 gttaaaaaat tcatgcgtgt tcccaagggg gctggtaact cggtgccatg ggatcctgaa
      721 ctgacaccct acatcattga gcccatgaac tgcctggcat cgcgtgaata cgatgcggtg
      781 atttttgttg gtcctgcgcg aacagggaag accattggtc tgatcgatgg atggattgtc
      841 tataccatcg tttgcgatcc ttcggacatg ctcgttgtgc agatgaccga agataaggcc
      901 cgcgagcatt ctaaaaagcg cctcgacaga acgttcagaa gcagtgcggc ggtaaagaaa
      961 agaatgagtc cacgtcgtaa cgacaataat gtccatgata agacgttcag ggatggctcg
     1021 ttccttaaaa ttggttggcc ctcggtcaac attatgtcgt cgtcggatta ccggtttgtc
     1081 gccttaaccg attacgaccg ttttccggag aatatcgaca gcgagggtga tggtttttcc
     1141 ctggcctcaa aacgtaccac cacatttatg tccgccggga tgactctggt ggagagctcg
     1201 ccgggacgtg acatctgcga cagcaaatgg cgacgtaagt cgcctcatga agcgccaccg
     1261 acgactggta ttctttccct ttacaatcgt ggtgaccgcc gccgctggta ctggccatgt
     1321 ccgcactgcg gtgaatattt tcagccagct atggatgcca tgaccggcta ccgtaatgaa
     1381 ccggatccct ttaaagccag tgaggcggcg tatctacttt gcccgcactg cagcggcatt
     1441 atcactgcgg agaaaaagcg tgagctcaat agtgcaggag tctggttacg tgaaggtcag
     1501 gtcattgatc gtaacggcaa cgtttccggt gaaccgcgcc gctcccgtat cgccagtttc
     1561 tggatggaag ggccagctgc tgcgtatcag acctgggcgc aactggttta caaattactg
     1621 actgcagaac aggagtatga agcgacagga agcgaagaaa cactcagggc ggttatcaac
     1681 accgactggg gattgcctta tcttcctcgt gccagcatgg agcaacgaaa aagtgaactg
     1741 cttgagcagc gggcagagcc agttccttcc cgcagtgttc cggatggcgt taatttcctt
     1801 gtggcgacag tggatgtgca ggcgggacgt catcgccgtt ttgtggttca ggtaacgggc
     1861 tatggcagcc gtggcgaacg ctggattatt gatcgttaca acatcacgca gtcattgcgc
     1921 agtgactgcg acggggagag ccagcgaatt gatccggcca gctatccgga agactgggat
     1981 gtcctgctga cggatgtttt tcataaaagc tggccgctgg cctccgatcc ttctcaacaa
     2041 atgcgactga tggcaatggc ggtggattcc ggcggtgaag acggggtcac tgataatgcc
     2101 tataaattct ggcgtcgttg ccgtcgtgat ggccttggta aacgtattta cctgtttaag
     2161 ggcgacagca tccggcgcgc aaaactgatc agccgtacat tccctgataa caccggacga
     2221 acgggccgcc gggcgcaggc cgcaggtgat gtgccgctct ggcttcttca gacggatgcc
     2281 ctgaaagacc gggtgaataa cgcgttatgg cgtgactcgc caggtccagg ctatgtgcat
     2341 ttccctgact ggctggggag ctggttttac gacgaactga cgtatgaaga gcggagcagt
     2401 gacgggaaat ggagtaagcc gggtcgcggt gccaacgaag cttttgacct gatggtgtat
     2461 gccgaggcgc tggtcattct gcatggatac gaaaagatcc gctggccgga tgcaccggag
     2521 tgggcgagcc gggaaacctg gctggagtgt gtcccggaca gtaccgaacc gtcaccctca
     2581 ccggagccgg tatccacgcc tgttaaaaaa caaaaatgga agaaaacagt aactgacgat
     2641 gttaacccct ggctgacttc cggaggatgg ttatgaatca gaatgatatt gaagccatga
     2701 ttcagcgtta tacggaagct gaaatggcgg tgctggacgg aaaatccgtc acctttaatg
     2761 gtcagcagat gaccatggaa aacttatctg agatccggca gggacggcag gagtgggagc
     2821 gccgccttgc ggctctgatt acacgacgac gggggcatcc cgggtaccgg ctggcgaggt
     2881 tctgatggca attcttgatg atgtgattgg cgttttttca ccaggatgga aagcggcaag
     2941 gctgcgttcc cgtgcggtga tccaggctta tgaggccgta aaaacgacac ggacacacaa
     3001 agcccggcgg gaaaaccgaa ctgccgacca gttaagccag tacggggccg tgtcgttacg
     3061 tgagcaggcc cgttaccttg ataacaacca cgatctggtt attggtgtat ttgacaagct
     3121 ggaagaacgg gtggtgggga aaaacgggat tattgtcgag ccacatccgg tattacgcaa
     3181 tggggccatt gcccgtgatc tggcagcgga gatacgcacc cgatggagtg aatggtctgt
     3241 cagtccggaa gtcaccgggc agtttacccg tccgatgctg gaacgtctga tgctgcgtac
     3301 ctggctgcgc gatggtgagg tgtttgccca gatggtttcc gggcgcataa acagcctgac
     3361 gccttctgcc ggtgttcatt tctggctgga ggcgctcgag ccggacttta ttcccatgac
     3421 cagtgatgag agcaacaggc tgaatcaggg cgtgtttgtt gatgactggg ggcgtcccga
     3481 aaaatatctg gtgtataaaa gccgtcccgt atccggacgg cagatggaaa ccaaagaagt
     3541 ggatgcagag cgaatgctgc atcttaaatt tgttcgccgt ctgcaccaga tgcgcgggac
     3601 gtctttattg tccggtgtgc tgatccgcct cagtgctctg aaagagtatg aagattctga
     3661 gctgactgca gcaaggatcg ccgctgctct ggggatgtac atccggaaag gcgacggaca
     3721 gagctatgaa acggatggta atgacagcaa ggagaatgaa cgcgagctta ccattcagcc
     3781 aggcattatt tacgacgatc tgaaacccgg cgaagaaatc ggaatggtga agtcggatcg
     3841 tcccaatcct aaccttgaaa cttttcgtaa tggtcagttg cgtgccgtgg cggcgggcag
     3901 tcgtctgagt ttttccagta cggcacgcaa ctataacggc acttacagcg cccagcgtca
     3961 ggagctggtt gaatccactg atggctacct gatcctgcag gactggttta ttggtgccgt
     4021 cacccgcccg atgtatcgtg cctggctgaa acaggctgtg gcatccggtg ttatcaggct
     4081 accccgcgat cttgaccgtt cttcactgta taccgcggtg tattccggac cggtgatgcc
     4141 gtggattgac cctgttaagg aggctgaggc ctggaaaatt cagattcgtg gtggagcggc
     4201 gacagaatca gactgggtac gtgcaggtgg tcgtaatccg gatgatgtca aacgtcggcg
     4261 caaggccgaa attgatgaaa accgcaagct ggatctggta tttgataccg atccggccag
     4321 tgataaagga ggcagcagtg ccgcaacgaa acgacagtag ccgcagcaca ccgacgacca
     4381 gtccgaagaa taattcctgg ttcaggatgc aggctggtca ccagagtgac gcggatattt
     4441 atatttatga cgagattggt ttctggggtg ttacagcgaa gcagtttatc agtgatctga
     4501 atgcactggg cgatatcacc cacattaatc tccatatcaa ttcaccgggt ggcgatgtct
     4561 ttgaaggcat cgccattttt aatgcactga aaacacatgg tgcgtccatt accgtttatg
     4621 tcgacggtgt ggcggcgtca atggcgtcgg tcattgcgat ggtgggaaac ccggtcatta
     4681 tgccggaaaa caccttcatg atgattcata aaccatttgg ctttacgggc ggtgatgcgg
     4741 aggacatgcg cacctatgcc gacctgctcg ataaggttga ggcggttctg ttacccgctt
     4801 atgcacagaa aaccgggaaa accaccgatg aaattgctgc catgctggcg gatgagacct
     4861 ggatgtccgg tgccgaatgt ctggcacatg gatttgctga tcaggtgacg ccagccgtta
     4921 aggcaatggc atgtattcag tcaaaacgta cagaggaatt taaaaagatg ccggaatcca
     4981 ttcgaaacat gattactccg ccacgcaaca gtgctccacg cgtacaggat gatgaacctg
     5041 cagcctcccg gacgccagtg caggcagcag cacccgtggt ggatgaaaac agtatccgtg
     5101 cgcaggtact ggcagagcaa aaagcgcgtg taaacggtat taatgatctg tttgccatgt
     5161 ttggcgggcg ttatcagacg ctgcaggctc agtgtcttgc cgatcctgaa tgttcgctgg
     5221 agcaggcccg cgaaaagctg ttgaacgaga tggggcgcga gtccacgcca tccaataaaa
     5281 atacaccggc tcatatttat gccggtaacg gtaattttgt gggggacggg atccgccagg
     5341 cgctgatggc gcgtgccgga tttgaaaaaa ccgaacgtga taatgtctac aacgggatga
     5401 ccctgcgtga atatgcccgt atgtcactga ctgaacgggg tattggggtt tccagttata
     5461 acccgatgca gatggtcggt gcggcgttca cacacagtac gtctgacttc ggtaatattc
     5521 tgctggatgt tgcgaacaaa gccattctgc agggctggga agatgcccct gaaacctatg
     5581 aacagtggac gcggaaaggt cagttgtctg attttaaaat tgcccatcgt gtgggtatgg
     5641 ggggcttcag tgctctgcgt caggtgcgtg aaggggcgga atataaatac gtcaccaccg
     5701 gagataaaca ggccactatt gcactggcga cctatggcga gctgttcagt atcacccgtc
     5761 aggccattat caatgatgat ctgaatatgc tgaccgatgt cccgatgaag ctgggccgtg
     5821 cggcgaaatc cactattgcc gatctggttt atgccattct gacgtctaac ccgaaaatct
     5881 ccacagataa tgtaagtctg ttcgataaag cgaaacatgc aaacgtactg gagagcgctg
     5941 caatggacgt ggcatcgctg gataaagccc gccagttgat gcgcgttcag aaagaggggg
     6001 agcgtcatct gaatattcgt cctgcgttcg tactggtacc gacggcgatg gagtctgttg
     6061 ctaaccaggt cattcgctcc tcaagtgtca agggggctga cattaacgcc ggtattatta
     6121 acccggtgaa agattttgcg accgttattg cagagcctcg tcttgatgat aacagccaga
     6181 ccaccttcta cctggctgcg tcaaaaggct ccgatacgat tgaagtggct tatctcaacg
     6241 gtgtggatac gccatatatt gatcagatgg agggcttcag tgtggatggc gtgacaacga
     6301 aagtgcgtat tgacgccggt gtcgcgccag ttgatcaccg cggtctggtg aaatgtacgg
     6361 cgtaaacgtc gcagacaaca actctgatgg cccgtaaggg ctttttttgt acctgaaatc
     6421 agcccctgaa cggggctgtg cggagacagt tatggcaaag aattttgtag aagaaggaaa
     6481 aacggtggcg attgttgcca gtgcagccat cagcagcgga gatctggtgc aggtgggtga
     6541 tgtttttgcg gtggcgctga ccgatattcc acagggtgaa acaggcgacg gcatgaccga
     6601 aggtgtgttt atgctgccta agctgaaaac ggatgacatg aaaacgggta agaaggttta
     6661 tctgaagtcc ggaaaagttc agctgactaa cagcggctct gatccgctgg tcggggttgt
     6721 ctgggcagat gccggaacca gtgcagaaga agtgccggta aaactcaatg tctgatccct
     6781 tttcccggct ggcagcgcgt atggatgcga tcacggtcag aaagatggga aagacagcct
     6841 cgattaatga tgccgatatg actgtgatcc cgggagaaac actggcagag ctgaatgctc
     6901 tgtccggacc tgcggtatct ctggtggtgt tttcttcggg ataccgccca cggcgcgggg
     6961 atcgcgttgt ttatgacgga caacaatgga cggtcacacg gcatgaacgt tttagcggta
     7021 agccaatgat ctttattgag taaagaggtg tgggatgaag gggcttgaga atgccatccg
     7081 taatctgaac agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg
     7141 tgtggcacag aaagcggtct cggtcgccac ccggcaggtt gccgggaata ccgttgcggg
     7201 agataaccag gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa
     7261 agccagtccg tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc
     7321 tattaagctg gggacagccc gggtcagact ggcccggcgt ggtggaaaac tgcagtaccg
     7381 tggcagtgtg ctgaaggtgg gtaaatatct tttccgggat gcgtttattc agcaactggc
     7441 gaatggtcgc tggcatgtga tgcggcgtat tgatggcaaa aatcgttacc ccattgatgt
     7501 ggtgaaagtc ccgctgtccg gaccgctgac acaggcattt gaagatgccc gcgaccgcat
     7561 tattgctgcg gaaatgccga aacagctggg gtatgcactg aaacaacaac tgaggttatg
     7621 gctgacccga tgaaccgaca tacacaaatc cgccaggccg tactggcacg ccttcgggaa
     7681 cagtgtggag acagcgccac gttttttgac gggcttccgg catttattga tgcgcaggaa
     7741 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgaa
     7801 gatgactggc aggctgttct gcatattgct gtcttcatcc gggaacaggc accggattca
     7861 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatat accggcactt
     7921 tccggactca tcgacaccct gatccctctc ggttttaact atcaacgtga taatgagatg
     7981 gccacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aaggaggtgg
     8041 caatgaccac accaaatcca ctggcaaaaa cgaaaggtgc gggaacgacg ttctggatgt
     8101 acaccggcaa gggcgatgcg tttgcgaacc ctttatcgga cactgactgg ctgcgtcttg
     8161 cgatggtgaa ggatctgcaa cctggcgaaa tgaccgctga tgcagaagat gacacttatc
     8221 tcgatgatga agatgcagac tggaaaacga caacccaggg gcagaaatcc gtcggtgata
     8281 cttcggcgac gctggcctgg cgtccgggtg acagcgggca gaaaaaactg gttcagttgt
     8341 tcgactccgg tgaagtctgc gcgtttcgta tcaaatatcc caacggcact gttgatgttt
     8401 tccgtggctg gctgagctca ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca
     8461 cagtgaaaat cagcggtgtg gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg
     8521 gcgttaccgg gctgacggtg gcaccggcat ctgccagtgt aaaagtggga gcaaccacca
     8581 cgctgacctt tacagtaaaa cctgacggag ccagtgacaa agcgatcagt gtgcattcga
     8641 cagatccaca gactgccacg gtgaccctga acgggcttgt ggccacggtg aaaggcgtga
     8701 agcagggcag tgtcagcatt gtgggcatga cttctgacag cgattttgtg gcagtggcta
     8761 cggtggctgt cagcgccgca ggttaacagg atgatactca tcatttgccc cggttatccg
     8821 gggctttttt gcaggtgtag aacatgatgt ttctgaaaca ggacacgttt aattatgaaa
     8881 aacagtccgt ggtgctcagt gagctgtccg ggctgcagag aattgaatat ctggcgtttg
     8941 ttcagcagcg aacggcaaag tttgatgccg aagagggaga actgccggag gctgaacgac
     9001 agattgcttt tctgcggatg gggatggata tcaatgcctg gctggtttcc cgctcactgt
     9061 ggaatgcgga acagtctcag gatgttgaga cgctttgcgc atccgttatt acaacatggt
     9121 cgtatgatgc cctgggagcg ggggcggaga tggttctgtc gctgagcggt atgggagtca
     9181 ttgagaatgc cggggatttg gagcatgagg tgctgacgcc ggaaaagtcc tgacgcggga
     9241 aatgcagttt gtcatgcggc ttgcccggga gttccggcgg gcagactggc ggcggatgct
     9301 gtcggaaatg tcggccactg agcttggtga gtggggcgat tatttccgga tgcagagctt
     9361 cagtgatgtg tggatggatg cgcagtttgc ctcgctgaag gcattgatcg tgagaatggt
     9421 gtccggcagc agtgatgctg cggtggctga tttcagcctt ttaccggaag agaacgggat
     9481 accggagcga acggacgaag aactgatgca tcttggggaa ggtatttccg gaggtgtgcg
     9541 ttatggacca gatagccaac ctggtcattg atttggggat tgatgcggca gagtttaaaa
     9601 atgaaattcc ccgtatcaaa aaccttctga atggtgcagc cagcgatgca gaacggtctt
     9661 ctgcccgtat gcagcgtttt atggagcgtc agactcaggc cgcccggcag acaacgcagg
     9721 cggcttcttc ggctgcaaca gccgcatccg tccatgcgca gacggtggag aagaacgcac
     9781 aggctcatga acgcatggcc cgcgaggtgg agaaaacccg ccagcgcatg gaggcactga
     9841 gccagaaaat gcgtgaggaa caggcgcagg ccatggctct ggcggaggct caggataaag
     9901 cggctgctgc gttttatcgt cagattgaca gtgtgaaaca ggccagtgcg gggctgcagg
     9961 aattacagcg tattcagcag cagatccgac agaccagaaa cagtggcggg attggtcagc
    10021 aggattatct ggcgctgatt tctgaggtta cggcgaaaac ccgtgttctt acacaggctg
    10081 aggaagaggc tacccgacag aaagtggcgt ttatccgtca gcttaaagag caggcaaccc
    10141 gccagaatct ttcatcttct gagttgcttc gtgccagggc agcccagctg ggggtaagca
    10201 gtgctgcaga agtgtatatc cgcaaaatgg agcaggcagg aaaagccacg cattcgctgg
    10261 gtctgaaaag tgcagcagcc cgccaggaga taggcgttct gataggtgaa ctggcccgtg
    10321 gcaatttagg agcgctgagg ggatccggga taacgctggc taaccgtgcc ggatggatag
    10381 acacactgat gtcaccgaaa ggcatgatgc tgggcggggt tattggcggt attgccgcgg
    10441 ccgtctatgg tctgggtaaa gcctggtatg acggtcagaa ggagggggaa gaatttaacc
    10501 gccagctgtc actgacgggg cattatgccg gagtcactgc cgggcagctg tggacgctca
    10561 gtcgtgctat ttccgggaat ggtatcacgc aacatgctgc agccggtgcg ctggctcagg
    10621 tggtggggag tggtgcattt cgtggaaacg atatcggtat ggtggcgaga gctgccgcac
    10681 agatggagcg atcggttggc cagtcggtca gcgataccat aaatcagttt aagcggctga
    10741 aggatgatcc tgtaaatgcc gcgaaggctc tggacaatga gctgcatttt cttactgcca
    10801 ctcagcttga gcagatacgc gtccttgggg atcaggggcg gtccagtgat gctgcacgga
    10861 tagccatgtc tgcactggca gaggaaaccg gtcggcgtac tgcggatatt gataataacc
    10921 tcaatgcgct gggcagtacg ctgaagtatc tgtctgattt atggagtcgt ttctgggatg
    10981 cggccatgaa tattggtcgt gaagactcgc tggatgaaca gattgccgct ttacaggaga
    11041 aagtgtcgcg ggcgaaaaga ctcccctgga cggcatcatc ttctcaggtt gaatacgatc
    11101 agcagcgtct taacgatctt caggagaaaa aacgccagaa ggatttgcag gatgcaaaag
    11161 agcaggcaga gcggaattat caggagcaac agaaacgccg taatgctgaa aatgctgcac
    11221 tgaaccggat gaatgaaacg gaagctgcac gacatcagcg tgaaattgcg cgtattaatg
    11281 ccatgcagta cgccgatcag gctgtcaggg atgcggcgat acaacgtgaa aatgaacgtt
    11341 acgagaaagc cctggcatcc ggtaagaaaa aaacacgcga aacccgtaat gatgaggcca
    11401 cccggttatt gctgcagtac agtcagcaac aggcacaggt ggaaggacag attgctgctg
    11461 ccagacagtc agcaggcatt gccacggaaa ggatgacaga agcgcataaa cagcttctgg
    11521 ctctgcagca gcgcatcagc gacctggacg ggaaaaaact gacggcagat gaaaagagtg
    11581 tgctggcccg taaagatgaa ctgattcagg cactgacgct gctggatgta aaacagcagg
    11641 agcttcagaa acagacggca ctcaacgagc tgaagaaaaa aacaattcag ctgaccagtc
    11701 aactggctga agaagagcgc gctcagcgtc agcaacatga cctggatatc gccacggtgg
    11761 gtatgggtga tcagcagcga cagcgatatc aggtacaact gagtcttcgc cagaaatacc
    11821 agcaacagct ggagcagttg aggcgggata gtgagcagaa agggacatat aacacggatg
    11881 actacagaaa ggccgagcag gcgctgacgg agagcctgaa ccgacaactg aatgagaatc
    11941 gccgttactg gcaacagctt gaagttgtgc agggtaactg gaaaaacgga gtcctgcgtg
    12001 catttcagga ttttaccgtg gatgcagata atacggcaga aacagcagaa caggtgttct
    12061 cgtcagcctt cagcaacatg ggaaatggcc tggcaacttt tgtcactacc ggcaaactca
    12121 atttcaaatc cttcacctct tctgtgctgt cagatatggc gaaaatcctg gcgcaggcaa
    12181 ccatgatgaa atcgataaaa gggattggca gtgtactggg atttgatctc agcagccttt
    12241 ccctgaatgc caatgggggg atttatcagt ctgctgattt gagtcgttac agtggcacgg
    12301 tggttaaccg tccgacgttt tttgcttttg caaaaggcgc gggtgtgatg ggggaagcgg
    12361 gacctgaagc cattctgcca ctgcgtcgtg gtgctgacgg taagctgggg gttgtggcgg
    12421 atattggggg ttcaggtatg gc
//
LOCUS       DAYPHN010000143        12390 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000143 DAYPHN010000000
VERSION     DAYPHN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.143"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            120..1013
                     /locus_tag="ACG0BW_003731"
     CDS             120..1013
                     /locus_tag="ACG0BW_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7260641.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1044..2033)
                     /locus_tag="ACG0BW_003732"
     CDS             complement(1044..2033)
                     /locus_tag="ACG0BW_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFK7260642.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2060..2911)
                     /locus_tag="ACG0BW_003733"
     CDS             complement(2060..2911)
                     /locus_tag="ACG0BW_003733"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFK7260643.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINIGYRLID
                     TAASYQNETQVGNALKRSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            3477..7730
                     /gene="fdeC"
                     /locus_tag="ACG0BW_003734"
     CDS             3477..7730
                     /gene="fdeC"
                     /locus_tag="ACG0BW_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFK7260644.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVIADNNVEKNFASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAVSAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTLTAIWSTKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKSNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGTLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSDAQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSEVTLTASPANLALDPDGTAKTNEQGQA
                     IFTATTTVAAKYTLTAQVSQTNGQVSTKTAESKFVADDKNAELTASSDVQSLVADGKS
                     TAKLEVTLFSANNPVGGNVWVDIEAPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMK"
     gene            complement(7851..8708)
                     /locus_tag="ACG0BW_003735"
     CDS             complement(7851..8708)
                     /locus_tag="ACG0BW_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7260645.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            8956..9825
                     /locus_tag="ACG0BW_003736"
     CDS             8956..9825
                     /locus_tag="ACG0BW_003736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFK7260646.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAKAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(9985..10578)
                     /gene="rclC"
                     /locus_tag="ACG0BW_003737"
     CDS             complement(9985..10578)
                     /gene="rclC"
                     /locus_tag="ACG0BW_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFK7260647.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(10590..10826)
                     /gene="rclB"
                     /locus_tag="ACG0BW_003738"
     CDS             complement(10590..10826)
                     /gene="rclB"
                     /locus_tag="ACG0BW_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFK7260648.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(10935..12260)
                     /gene="rclA"
                     /locus_tag="ACG0BW_003739"
     CDS             complement(10935..12260)
                     /gene="rclA"
                     /locus_tag="ACG0BW_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFK7260649.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3317 a         2832 c         3046 g         3195 t
ORIGIN      
        1 ttctatacgg ccataatatc aattatcatt atcaccggta atgtggttaa aataatagca
       61 tctatgctcc ccccttcatg agtgtctgtg aagaggatgc ttctacaagg aaatcgccaa
      121 tgctcaaaga aaacttcaac gaactgcaaa tctttcttgt ggtggcaaga gagcgaagtt
      181 ttaccaaagc agcgggcaaa ctcggcgtct ctcagtctgc acttagccat gcaatgaagg
      241 ctctggagga aaggctcaac atccgcctcc tgacacggac tacccgcagt gtggctccca
      301 cagaagcggg ggagagaatc attgcctgcc ttgaaccgcg tattgacgag cttgaacagg
      361 aactggagtc acttattcaa ctgaacggca cgccctccgg gaatattcgt ttatctgccg
      421 gggaacatgc cgcgcgaagt ctggtttggc cgaagctaaa accttttctc agagagtatc
      481 ccgaaatcaa tgtcgaactg gtggtggata acggttttgt cgatattgtt gaaggccgtt
      541 ttgatgccgg gatacgactg ggtgaaaatg ttgataaaga tatggttgca gtcaggattg
      601 gaccagatat gcggatggct gtggtgggag ctccggcgta ttttgctgca aaccctgcgc
      661 ctgaaacgcc tcacgagcta caaaatcatc gctgcatcaa catgcgtttg ccaactgccg
      721 gtgggcttta ccactgggag ttcgagcgag aagggaaacc tcttcgcgtt aaggttgatg
      781 ggcagctgac gtgtagtcca ttgccagaaa gaattgatgc cgcgttatca gggtttggga
      841 tcgcttgtgt ccctgaagac atggttcagg agtatataaa gtcaggcaag cttattcagg
      901 tgttacagga gtggtgtcca acttttcccg gatattatct ttactacccc agccgcaagc
      961 agcatccgcc agcttttgcg ctgttgatcg atgcgcttcg ctacaccgaa taacgggtcc
     1021 tttatcctgg acccgtttgg gtattagcgg cctacgcgtg cctgatgttc aggcgagtaa
     1081 cgttcaccga cgattttaat tgtttccagc gcctcggtta tctgctgcgt atctttctgc
     1141 gaaagaacaa tgtcagcagc ggccaagttt tcttccagcc ggttcagttt agtggtgcca
     1201 gggatgggaa caatccacgg cttttgtgct aatagccatg ccagtgcgat ttgtgcagac
     1261 gttacccctt tctccgcagc cagttcacct aacaaggtga ccagtttttc attggcttca
     1321 atggcttgtg ctgcgaaacg cggtacggtg ctgcggtaat catctttgcc gaaagtggtt
     1381 cccggcttaa tcgcccctgt caggaaacct ttacctagtg ggctgaaggg gacaaaacca
     1441 attcccagtt cctccagtaa cggcagaatt tcctgttcag gctcacgcca ccacatggaa
     1501 tattcgcttt gcagggcggt gacaggttgt accgcatgtg cacgacgaat ggtttgtgct
     1561 cccgcttccg acaggccgaa atgtttgacc ttaccttctg cgatgaggtc tttcaccgtt
     1621 cctgcaacat cctcaatagg aacatccgga tcaacacggt gttgatacag caggtcaata
     1681 acatctgttt tcagacgacg taatgatcct tcaacggctt cacggatatg ttccggacgg
     1741 ctgtttaaaa tctgctgctt attatcatcg ccgaaggtaa aaccgaattt agtggcaatt
     1801 accacgcggt cacgaaacgg ttttaaggct tcgccgacca cttcttcatt cagatacgga
     1861 ccatacactt cggcggtatc gaagaaggtg acgccacgtt caaccgcagc acgaataagt
     1921 tcgatagcct gacgggtatc ggtcgccggg ccgtagccgt ggcttaagcc catgcaaccg
     1981 agcccaaggg cggagacttc gagtccggat ttacccagat aacgtttttg cattgaatga
     2041 atacctcttt tatcgcggct taaacatcaa gtttgcggcc agtcagccat tccaccattg
     2101 cgggatcgcg gtgagagaag aacgcgctgg ttgcggtatc cagggcggta atttgcagca
     2161 tatcttcagg gctgagttca aaatcgagaa tgttgatgtt ctcttccatg cgttcttttc
     2221 gcactgattt tgccagtgaa acaatgcctc gttggtagat ccagcgcaga acaacctggc
     2281 ccacgctttt gccatacttc tcgccaattg ccgtgagaac aggatgctgg aacagaccat
     2341 ttttcccttc cgcaaacggt gcccaggctt ctggctgaat gccacgactt tgcatccagg
     2401 gaacggcctg tagctgctga ttgaaagggt taacttcaac ctggtttacc gccggaacga
     2461 cgttattaaa agcgataagg tctgccagtc ggtcaggatg gaagttactg acgccaatag
     2521 cgcgaatttt accggcctgt tgcagctctt ccatggcacg ccaggctccg tggacatcgc
     2581 cgtatggctg gtgaatcaaa tacaggtcaa cgtaatcaag ttgcagtcga ttcagagagc
     2641 gttcgaactg ggctttggcc ccttcgtaac tggtatcctg caaccagagt tttgtggtga
     2701 caaagagttc gttacgggcg ataccgcttc gtttcagtgc gttcccgacc tgggtttcat
     2761 tttgatagga ggcggcggtg tcaatcagtc gataaccgat gttaatagcg tcaatgaccg
     2821 ctctttcgca ttcggcggca tcggtcatct gaaatacacc aaagcccaac aggggcattt
     2881 caataccgtt gttcagtttt actttttgca tggtgttatc cttccgttat cgttcatgag
     2941 aagcataacg taaagggaag agctcgatta gacggcagga tttgtcaggg gttatgaacg
     3001 aaattcataa atctgcttga gtgttgcgat gagtcgtgca agttcgatat ccctgcaatt
     3061 cacagtctga tgaagaaaaa tgaatatcca taaaaaatat attggtttat cctggtgtat
     3121 atacctactt cgacgtattc ccaatagcga ttaattaaag acaggtcatt attattcact
     3181 cttaataatg aattattcac tgcccgcaga gtaagaaata taacttaagt atctatttaa
     3241 taacttgcac aaaaagctaa attttcacca ataaataaaa atataatccc gcgcccaacc
     3301 acctgatgag tggctacaga cactggatat attaggtggc ggtgtacttt cttacataaa
     3361 ggtatttcct tttctgcgga aaaggaaatc ggggatttcc cggtttttct gacaagcaga
     3421 cgccactatt tgtgtctgcc tatgttcgtt aattcgttca tcaggaaatt atctcaatgt
     3481 cacgttataa aacaggtcat aaacaaccac gatttcgtta ttcagttctg gcccgctgcg
     3541 tggcgtgggc aaatatctct gttcaggttc tttttccact cgctgtcacc tttaccccag
     3601 taatggcggc acgtgcgcag catgcggttc agccacggtt gagcatggaa aataccacgg
     3661 taattgcaga taataatgtg gagaaaaatt tcgcgtcgtt tgccgcaaat gccgggacat
     3721 ttttaagcag tcagccagat agcgatgcga cacgtaactt tattaccgga atggccaccg
     3781 ctaaagctaa ccaggaaata caggagtggc tcgggaaata tggtacagcg cgcgtcaaac
     3841 tgaatgtcga taaagatttc tcgctgaagg attcttcgct ggaaatgctt tatccgattt
     3901 atgatacgcc gacaaatatg ttgttcactc agggggcaat acatcgtaca gacgatcgta
     3961 ctcagtcaaa tattggtttt ggctggcgtc atttttcagg aaatgactgg atggcggggg
     4021 tgaacacctt tatcgaccat gatttatccc gtagtcatac ccgcattggt gttggtgcgg
     4081 aatactggcg tgattatttg aaattgagcg ccaatggtta tatccgggct tctggctgga
     4141 aaaaatcgcc ggatgttgag gattatcagg aacgcccggc gaatggctgg gatattcgtg
     4201 ctgagggcta tttacccgcc tggccgcagc ttggcgcaag cctgatgtat gaacagtatt
     4261 atggtgatga agtcgggctg tttggtaaag ataaacgcca gaaagacccg catgctattt
     4321 ctgccgaggt aacctataca ccagtgcctc ttctgacact gagcgccggg cataagcagg
     4381 gcaagagtgg tgagaatgac actcgctttg gcctggaagt taactaccga attggcgaac
     4441 ctctggaaaa acaactcgat acagacagca ttcgcgagcg tcgaatgctg gcaggcagcc
     4501 gctatgacct ggttgagcgt aataacaaca tcgttcttga gtaccgcaaa tctgaagtaa
     4561 tccgtattgc tctgcctgag cggattgaag gtaagggcgg tcagacactt tccctgggac
     4621 ttgtggtcag caaagcaact cacggactga aaaatgtgca gtgggaagcg ccgtcattac
     4681 tggctgaagg tggcaaaatt accggtcagg gtagtcagtg gcaagtaacg ctcccggctt
     4741 atcgtccagg caaagacaat tattatgcgg tttcagcgat tgcctacgat aacaaaggca
     4801 atgcctcgaa acgtgtgcag acagaagtgg ttattagcgg agctggtatg agcgccgatc
     4861 gtacggcgtt aacgcttgac ggtcagagcc gtattcaaat gcttgctaac ggtaatgagc
     4921 aaaaaccgct ggtgctgtct ctgcgcgacg ccgagggcca gccagtcacg ggcatgaaag
     4981 atcagattaa gactgaacta accttcaaac cggctggaaa tattgtgact cgtaccctga
     5041 aggccactaa atcacaggca aagccaacac tgggtgagtt caccgaaact gaagcagggg
     5101 tgtatcagtc tgtctttact accggaacgc agtcaggtga ggcaacgatt actgttagcg
     5161 ttgatgacat gagcaaaact gtcactgcag aactgcgggc cacgatgatg gatgtggcaa
     5221 actccaccct gagcgctaac gagccgtcag gtgacgtggt tgctgatggt cagcaagcct
     5281 acacgctgac actgacagcg gtggactccg agggtaatcc ggtgacggga gaagccagcc
     5341 gcctgcgact tgttccgcaa gacactaatg gtgtaaccgt tggtgccatt tcggaaataa
     5401 aaccaggggt ttacagcgcc acggtttctt cgacccgtgc cggaaacgtt gttgtgcgcg
     5461 ccttcagcga gcagtatcag ctgggcacat tacaacaaac gctgaagttt gttgccgggc
     5521 cgcttgatgc agcacattcg tccatcaccc tgaatcctga taaaccggtg gtcggcggta
     5581 cgcttacggc aatctggtcg acaaaagatg cctatgacaa ccctgtgacc agcctcacgc
     5641 cggaagcgcc gtcattagcg ggtgccgctg ctgtaggttc tacggcatcg ggctggacaa
     5701 ataatggtga tgggacgtgg actgcgcaga ttactctcgg ctctacggcg ggtgaattag
     5761 aagttatgcc gaagctaaat ggacaggatg cggcagcaaa tgcggcaaaa gtaaccgtgg
     5821 tggctgatgc gttatcttca aaccagtcga aagtctccgt cgcagaagat cacgtaaaag
     5881 ccggcgaaag cacaaccgtg acgcttattg caaaagatgc acatggcaac gctatcagtg
     5941 gtctttcgtt gtcggcaagt ttgacgggga ccgcctctga aggggcgacc gtttccagtt
     6001 ggaccgaaaa aggtgacggt tcctatgttg ctacgttgac tacaggcgga aagacgggcg
     6061 agcttcgcgt catgcctctc ttcaacggcc agccagcagc caccgaagcc gcgcagttga
     6121 cggtcatcgc tggagagatg tcatcagcta actctacgct tgttgcggac aataagactc
     6181 caacggttaa aacgacgacg gaactcacct tcaccgtgaa ggatgcgtac gggaacccgg
     6241 tcaccgggct gaagccagat gcaccagtgt ttagcggtgc cgccagcacg gggagtgagc
     6301 gtccttcagc aggaagctgg acagagaaaa gtaatggggt ctacgtgtcg accttaacgc
     6361 tgggatctgc cgcgggtcag ttgtctgtga tgccgcgagt gaacggccaa aatgccgttg
     6421 ctcagccact ggtgctgaat gttgcaggtg acgcatctaa ggctgagatt cgtgatatga
     6481 cagtgaaggt taataaccaa ctggctaatg gacagtctgc taaccagata accctgaccg
     6541 ttgtggacag ctatggtaac ccgttgcagg ggcaaaatgt gacgctgacc ctgccgaaag
     6601 gtgtgaccag caagacgggg aatacagtaa caaccaatgc ggcaggtaaa gcggatattg
     6661 agttgatatc aaccgttgca ggggaacttg aaatcgcggc cgcggtgaaa aactctcaga
     6721 agacggtcac ggtgaaattc aacgcggatg ccagcaccgg tcaggcaaac ctgcaggttg
     6781 acaccgccgt tcaaaaagtg gcaaacggca aagatgcctt tacgctgacg gcgaccgttg
     6841 aggataaaaa cggtaaccct gttccaggca ctctggtgac ctttaatctg ccccgaggtg
     6901 tcaagccgct tactggcgat aatgtctggg tgaaagccaa cgatgagggg aaagcagagt
     6961 tgcaggtggt ttccgtgact gccggaacct atgagatcac ggcgtcggca ggaaatgacc
     7021 agccttcgga tgcgcagact ataacgtttg tggccgataa ggcgaccgca accgtctccg
     7081 gtattgaggt gattggcaac tatgcactgg cggatggcaa agccaaacag acgtataaag
     7141 ttacggtgac tgatgccaat aacaacctgt tgaaagatag cgaggtgacg ctgactgcca
     7201 gcccggcaaa tttagctctg gatcccgatg ggacggcgaa aactaatgag caagggcagg
     7261 ctattttcac cgccacgacc actgtcgcag cgaaatatac actcacggcg caagtgagtc
     7321 agaccaacgg tcaggtatca acgaaaactg ccgaatctaa attcgtcgcg gatgataaaa
     7381 acgcggagct caccgcatca tctgatgtgc aatcgctggt ggcggatggg aaatcgactg
     7441 cgaagctgga ggttaccctg ttctcggcaa ataaccctgt tggggggaat gtgtgggtcg
     7501 acattgaggc tccggaagga gtgacggaga aggattatca attcctgccg tcgaaaaatg
     7561 accatttcgt gagcggaaaa atcacgcgta catttagtac cagcaagcct ggtgtctata
     7621 cgttcacatt taacgccctg acctatggcg ggtacgaaat gaagccagtg acggtgacca
     7681 taaccgcggt ggatgccgat acggcaaagg gcgaggaggc gatgaagtaa tattaagaat
     7741 atgaaagtaa cattctaata aacaataatg gcgttgacat cttcaacgcc attatttatt
     7801 gaattgcctg acagtagtct cgttataaat tcatgtaaac atcgctacag ctaaaccggg
     7861 cgaacgggga taaaaatttc gcaaacaatg ttattgctat caatttcgac gaagttttca
     7921 ttgtaataaa atcgctcaat atcactgccg ttcactctgg ctaaccccct ggcaggtaat
     7981 tccatcagat agaggtttcg cgcccattca gggtagtgct cccactcacc ttcatagcgg
     8041 aaagaggcat acagtcctcg gttaattgtc cactctttcg acatctctga atcggtttgt
     8101 ttgcataccg taccggcaaa agtttcaact gtgagcaaat cgaccgattt tgttgacggt
     8161 tcgaatgtgg agacgaaata gatatccgtc ttaaaattct tcatacatct ggctaaatga
     8221 tgacgtcgca gtttgacttc ttcatccggt gatttattcg atacttcaat atcatattta
     8281 aatgaattgc caaaaatagg tgtctcagga aagttgacta atctacactc cgtcttttgc
     8341 tgttgacgta ttaaaaaaga agggaagata tttgcgagat cccaataatc acggtggcgg
     8401 tattcacggg gagagcagcc gaataacttt ttaaattcgc ggctgaatga ctgctgtgag
     8461 tcaaaatgta aagagagtgc aatatcaagc atagatttcg cggtgagtcg gacaagaatg
     8521 gcggcacgac aaagccttcg tttgcgaatg tattctccca aaggcacatg catgaaattt
     8581 cggaacagaa gctggatgtt gcgtctgctg tagccagatt tctgtgcgat atcttcgatt
     8641 gaaatagggt gctcaagatt gcactcaatc cactccagga gctgctgtag aatcttctgc
     8701 ctgatcatca cattctccgt taattctttg tcatatcgca cattttcgtt atgtcctggg
     8761 ccttaagtgt atcaaaaaca gcaagtccca aggcaaaagc actgatagcg tctgcttcat
     8821 tgccccaaaa atagagaggt ttcagtccta cattattaat gaattttttg cataagtgat
     8881 atcaaaatcc acatactaat ttgaggttac gttttaacgt agactcattg ttcataccta
     8941 atggagggac tgacagtgga attttccgta ttaagtaaca atctgaaaat gccgatgatg
     9001 ggatttggtg tttttcaggt taccgataaa gacgtgtgca aacagtcagt gttgaatgct
     9061 atccgcacgg gttatcgact catcgacact gcagcggtat acggtaatga agatgccgtc
     9121 ggtgaagccg ttcgtgaagc tatatctgaa ggtttatgta cccgagatga gttatttata
     9181 acgtctaaat tgtgggtgca ggatatgttg aatcaggata cggcaaaagc aggtattgaa
     9241 gcatcattaa aaaaatcggg actagaatac ttcgacctct atttattgca ccaggctatg
     9301 cgcgattatt tcagtgcgtg gcgtgcactc gaagatgcct atgaagaggg caaattaaaa
     9361 gcaattgggg tttccaattt ctatcctcat gttctggcga acttttgtga aacggtaaga
     9421 gttaaaccga tggtcaacca ggtcgagttg catccctatt ttgcccaacc agaggcgctg
     9481 gcaaccatga agtattataa cgtgcagcct gaagcatggg ctccgttagg tggtggacga
     9541 cataaaccct ttgaaaataa tctgcttcag agtattgcag atgcccatca aaaatcgatt
     9601 tctcaagtca ttctgcgttg gaatattcaa cggggagtgg tcgttattcc gaaatcgaca
     9661 catcaacagc gtatcgaaga aaattttgct atctgggatt tctcactgac agagaaagaa
     9721 atggcacaaa ttagttcgct tgatttgggt tatgttgggg agtcggtaaa acattttaat
     9781 cctgaatttg ttcgtggttg tcttgctgta aaaatacatg attgatatta atcatgtatt
     9841 ttacctgaga cgacaagaat cttttaacag gggagtgata ttgatcttca ctctgtcata
     9901 tctccggtaa tatggcgtca ggcttcatca tcagaaaggg ggcctgacgc ctgaaaaagt
     9961 gaacaacaga cagtgttcgg attatcaata ttcagttttt aacgttgaac tggattcatt
    10021 actgcgttgt ttaagaattt cccgcgccga atctgccatt atcatgacag cacctgccag
    10081 catcagagta tctttcaata ccaggcgacc agcaccggat aaataaggga aaccatgatg
    10141 agcgtcaccc aatgcgggta cccatgcctc cggggtggtg attaaaaatg agagtgttac
    10201 caacggtgtg gtaaatgcca tcagccctcc caataaacct aaccagcgat tgacaggatt
    10261 agccaaaacc agcagagcaa taatcacctc cacgacgcca agaccgttgg aaaaaccata
    10321 ggtattattg gccgattgcc atgcccttgc ttctggtttg tattcgcctt cgtgagtcag
    10381 atactgttta tagtcttccg ggtgttcata aaagaacgac attagtggac tgtttgcgac
    10441 gaatggtgta atgctgtctg cctcgtaagg gacaaacttt aataacccaa tccacataaa
    10501 aacaatcgca atactcagac gaatcaatgt caggccaatt ttatctcccc gacttaacag
    10561 gtgaaggtat ttttccattg tgaacatcct tatttataca atactgctgt tgcggttgaa
    10621 ttattatcaa ttcgcatcga tgtgactttc caggatgaag cattatattt tctggccata
    10681 tcagcaatgg catcctccag aactgtcggt gatgctgata ctgacgagac gctgatatgt
    10741 ttcagaatta ttttatcatc tgcattggtg gaaaatgcca taacgccaga taatagtgtt
    10801 gcaaataaaa cagatttttt aaacatataa aataccatca atgaagtgat taagaagtta
    10861 tagagtaaca gagaattaat aagattcttt tctctgagtc gccagaatat ttgttctggc
    10921 gtctgatttt gagtttattt gactaatgaa aatagatcat tgagtgattc gctcatcgac
    10981 ggatgagtaa atatctgatc gcgtaatatg ctataaggca gcccggcatc catcaccatt
    11041 ttcactatat taatcatctc gtgggagtca acacacagca gtgatgcccc taatatacgt
    11101 tgggttttat tatcaacaat cgcttttaat accccacggg tatcgttcat cactctggcg
    11161 cgcggaattg cagctacagg caatgtcacc acctgaatat cagcaccact ctctctggct
    11221 tgttcttctg tcataccaac cctggacagg ggcggtgtca taaatacgga ataaggcaca
    11281 tttttccgat catcagtact acgtttgcct tcacccagta actcatcacg tacaatgcgg
    11341 taatcatcca gtgaaatata agtaaattgc agcccgccgg taacatctcc catcgcccaa
    11401 atattgtctg cggtggtatg taatcgcttg tcaacgacaa ttgccccgcg ctcgtttact
    11461 gcgataccgg cattttctgg atgtaacgaa gcggtagccg gttgacgacc ggaagctatt
    11521 aacagtgcat ccaccgccag ttgggcgtgc tcgctatgca cttgcacttg attttcatgg
    11581 tgactgattc gctccacatg ggcattgagg ataatatcga cgccctgatc gcgtaaaatc
    11641 gtcgcgatat tatcagcaat atcccgatct tcccgaggca aaaacagcga agctgcttct
    11701 aaaatggtta ccttgctgcc aaaattagcg aacatagagg cgaactcaac gccaatatat
    11761 ccgccgccta aaatacctaa atgcccaggc aattctttta gattaagtaa tccggtgctg
    11821 tcatatactc ctggcgtggt ggtaattcca ggaattggcg gaaccacggt ttgtgcaccg
    11881 gtattaataa aaattttctc gccatgaatt tccagatttc cctcaggccg atgaacacgc
    11941 aggctatgat tattgataaa ctccgcctgg ccgtcgatca cgtcgatatt gggcatatcc
    12001 gcaagattat gaaaattctt attacgtaaa aaattaacca cttcattttt acgctgtatg
    12061 gcacggacaa aatctgtgtg ctgctgtgcg tcatgaacca atgttttggt tgggatacag
    12121 ccgatattaa tacaggtccc gccatacatt gcatttgatt gttcgatgag agccacacgc
    12181 caacctgctt ttgccagcgt gacggctaat gtttttccag ccttgccaaa accaataata
    12241 actgcctgat atttattcat gatgatttcc tgtcgaaacg cgttcatttg acatcatgac
    12301 tatagacgca aaagagcaaa taacatttct tcacaacgct tgatattttg tcttatcgtc
    12361 tggtattttc tttttcttaa tcagaatcac
//
LOCUS       DAYPHN010000144        11957 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000144 DAYPHN010000000
VERSION     DAYPHN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.144"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..405)
                     /locus_tag="ACG0BW_003740"
     CDS             complement(<1..405)
                     /locus_tag="ACG0BW_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFK7260650.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLAL"
     gene            complement(479..769)
                     /locus_tag="ACG0BW_003741"
     CDS             complement(479..769)
                     /locus_tag="ACG0BW_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFK7260651.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(766..1467)
                     /locus_tag="ACG0BW_003742"
     CDS             complement(766..1467)
                     /locus_tag="ACG0BW_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFK7260652.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1464..2363)
                     /locus_tag="ACG0BW_003743"
     CDS             complement(1464..2363)
                     /locus_tag="ACG0BW_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFK7260653.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AGVTAGKPKLDLTNTDWIYGVDL"
     gene            complement(2396..2695)
                     /locus_tag="ACG0BW_003744"
     CDS             complement(2396..2695)
                     /locus_tag="ACG0BW_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFK7260654.1"
                     /translation="MEQTNYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITNKKRPVCKTERSEQIQMEF"
     gene            complement(2802..3029)
                     /locus_tag="ACG0BW_003745"
     CDS             complement(2802..3029)
                     /locus_tag="ACG0BW_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7260655.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            3108..3815
                     /locus_tag="ACG0BW_003746"
     CDS             3108..3815
                     /locus_tag="ACG0BW_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFK7260656.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTFSIVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTIEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            3945..4844
                     /locus_tag="ACG0BW_003747"
     CDS             3945..4844
                     /locus_tag="ACG0BW_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260657.1"
                     /translation="MSQELSLTFTENIYYSTKEPVSIKDVITSLQGWEAIAKQSEGVL
                     QELTGANILDISVHVARLEAGSLYEDIVIKLLFGSQEEMDKFLAGAHAKIGNGKMRNA
                     LVGAVVIGLVGYGLVLATKAMAPSNTSHFEANNNTIINIGAGEANISPDRLQAIIEST
                     VTNKKTLAKSSIKTLAPARADEGSTMVIGTGGGTVTIPAETIKKAPTEVVFTPESYTQ
                     DHFDVDVEIRALDLDNPEKGWAAVIPGLIDRRVNMVLGPNVNPSDFAGKFSVRADITI
                     TYQLKSSDKKYQPKEVFIKEIIK"
     gene            complement(5073..5288)
                     /locus_tag="ACG0BW_003748"
     CDS             complement(5073..5288)
                     /locus_tag="ACG0BW_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260658.1"
                     /translation="MMFSSCEMALVVMRQMLIFWLLSLLLQFTSPQTHLVWYLFALCQ
                     RIIEVKEPANLFRLAASVLLMAIIMNQ"
     gene            5287..5595
                     /locus_tag="ACG0BW_003749"
     CDS             5287..5595
                     /locus_tag="ACG0BW_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFK7260659.1"
                     /translation="MTVIVYGKSTFAGNAKTRRHERRRKLAIERDSICNIIDSIFGTD
                     SEEPVQEDPRKRLSLSEKAISLGSLRCKKVDECSGSVCLPNVAIYAAGYRKSKQLTAR
                     "
     gene            complement(5612..6193)
                     /locus_tag="ACG0BW_003750"
     CDS             complement(5612..6193)
                     /locus_tag="ACG0BW_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFK7260660.1"
                     /translation="MNNSWWQELMHFFLQGMTLKQLIHMLIILIILIIVMPVSVKEWI
                     NLHNPEILPHYWMYYILLFCVSYVLNGVVNSAYHAVTVRIEIFAAQKRKSKEEKYVQD
                     LFDSLTLGERAYLAFAVAANNQLKTEKGSPEAISLLEKGLLIRVPSATGYPEIDRFVI
                     PERYRNECYIRFAGKKDSLMDELIAQDKHGKNK"
     gene            6454..6822
                     /locus_tag="ACG0BW_003751"
     CDS             6454..6822
                     /locus_tag="ACG0BW_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFK7260661.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            6895..7059
                     /locus_tag="ACG0BW_003752"
     CDS             6895..7059
                     /locus_tag="ACG0BW_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFK7260662.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            7028..7171
                     /gene="kil"
                     /locus_tag="ACG0BW_003753"
     CDS             7028..7171
                     /gene="kil"
                     /locus_tag="ACG0BW_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFK7260663.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            7246..7542
                     /locus_tag="ACG0BW_003754"
     CDS             7246..7542
                     /locus_tag="ACG0BW_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFK7260664.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            7548..8333
                     /gene="bet"
                     /locus_tag="ACG0BW_003755"
     CDS             7548..8333
                     /gene="bet"
                     /locus_tag="ACG0BW_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFK7260665.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQDEAVKALGFLKQKATEQKVAA"
     gene            8330..9010
                     /locus_tag="ACG0BW_003756"
     CDS             8330..9010
                     /locus_tag="ACG0BW_003756"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFK7260666.1"
                     /translation="MTPDIILQRTGIDVRAIEQGDDAWHKLRLGVITASQVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            9007..9189
                     /locus_tag="ACG0BW_003757"
     CDS             9007..9189
                     /locus_tag="ACG0BW_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFK7260667.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            9162..9353
                     /locus_tag="ACG0BW_003758"
     CDS             9162..9353
                     /locus_tag="ACG0BW_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFK7260668.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPAETDEEFHTLAASL
                     SQKLEMMAAKAEANERELA"
     gene            9430..9645
                     /locus_tag="ACG0BW_003759"
     CDS             9430..9645
                     /locus_tag="ACG0BW_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFK7260669.1"
                     /translation="MTPQQKNALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            9744..9962
                     /locus_tag="ACG0BW_003760"
     CDS             9744..9962
                     /locus_tag="ACG0BW_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFK7260670.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEEIELKNKQWGL"
     gene            10010..10249
                     /locus_tag="ACG0BW_003761"
     CDS             10010..10249
                     /locus_tag="ACG0BW_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFK7260671.1"
                     /translation="MLRIIFPNTWYVDHHGTPCKILRSTYNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            10389..10625
                     /locus_tag="ACG0BW_003762"
     CDS             10389..10625
                     /locus_tag="ACG0BW_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFK7260672.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            10615..11757
                     /locus_tag="ACG0BW_003763"
     CDS             10615..11757
                     /locus_tag="ACG0BW_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFK7260673.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSEITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFESVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWVIVDIKTA"
     gene            complement(11871..>11957)
                     /locus_tag="ACG0BW_003764"
     CDS             complement(11871..>11957)
                     /locus_tag="ACG0BW_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HFK7260674.1"
                     /translation="VTYDFERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         3363 a         2759 c         2736 g         3099 t
ORIGIN      
        1 cagagccagt ctcgcttcgt ctgaccactt aacgccacgc tctgtaccga atgcctgtat
       61 aagctctaat agctccgcaa attcgcctac acgcatcctg ctggttgact ggcctattac
      121 cacaaagcca ttcccggcaa ggttaggaac aacatcctgc tgctttaatg ctgcggtaaa
      181 cacacacttc cagctttctg catccagcca gcgaccatgc cattcaacct gacgagagac
      241 gtcacctaag caggcccata gcttcctgtt ttggtctaag ctgcggttgc gttcctgaat
      301 ggttactacg attggtttgg ttgggtctgg aaggatttgc tgtactgcgt gaatagcgtt
      361 ttgctgatgt gctggagatc gaatttcaaa ggttagtttt ttcatgactt ccctctcccc
      421 caaataaaaa ggcctgcgat taccagcagg cctgttatta gctcagtaat gtagatggtc
      481 atcttttaac tccatatacc gccaataccc gtttcatcgc ggcactctgg cgacactcct
      541 taaaaatcag gttcgtgctc atctttcctt cccgttcttc cctggtagca aaccggtaat
      601 acaccgttcg ccagacctta ccttcgataa ccagaagacc tgcccgtgcc attttagccg
      661 cggcctgatt tatgctggtt actgttgcgc ctgttagcgc ggcaacgtcc ggcgcacaga
      721 agctattatg cgtccccagg taatgaataa ttgcctcttt gcccgtcata cacttgctcc
      781 tttcagtccg aacttagctt tgatttctgc gatcttcgcc agagcctgtg cacgatttag
      841 aggtctaccg cccatgacag gaagttgttt tactggttca gggatcgcct caccacggtt
      901 aattctcgca gtcatatgga caagctcatc tgcggcctta cggcgtaatt ccgcatcagt
      961 aagcgcattg gcccgcatgt tctgatacag gttggtaacc agccagtagt gcgcatttga
     1021 tttccacgga taagactccg catccggata caggcctcgc ttccggcaat actcgtaaac
     1081 catatcaacc agctcgctga cgtttggcag tccggcgata acggatgctt cttcccggca
     1141 ccatgcaaca aactgcccgg gtgatggcag aaatggtcga ttctgccgac gggctacgcg
     1201 cattcctgcg ttaacctgtt ccattgtggt gatcccgttt tcccggaaag ccagaaccca
     1261 ctggcggcgg atttcgttca gttcgttctg gtcacggtta gccaggctcg ccgggaaagt
     1321 tgccagtaac tggctgaaca caccgttgat gatctgcgct acctgctgta cctgtggctt
     1381 ttcgtcgtac tgttccggca tgttgttggc gatccgacgc atctgctcac ggtcaaagtt
     1441 aaccatctgt gcggcgatgt ttttcataga tccaccccgt aaatccagtc agtgtttgtc
     1501 aggtcgagtt ttggttttcc ggctgtcacg ccagcctgtt gcttgttacg gttgatttcg
     1561 agctgggtcc acttgtcgcg gagtttggcc ggactcagca cgttaccgga ccagaagttg
     1621 tcctggcagg cccagcggaa aagcacacac atatcgcggt ggttacgtcc gtcacgttca
     1681 cgcatcaggc ggatatcgtt agcccaccca gcaaaattcg gttttctggc tgatggcgcg
     1741 atggtcttca ccatgtcaaa catccactct gcggcggtca ggtcttctgc tgttccccac
     1801 ttactgccgc tctgaattgc agcatccggt ttaaccacag aaagatcgtt ttctggctgg
     1861 tcagaggatt cgccagaatt ctcggacgaa taatcttttc ttttttcttt tgtaatagtg
     1921 tcttttgtgt ccccctgttt tgagggatag caatccccca atttgaggga tgttttatcc
     1981 ctcgttttag gggattttcc ctcgttttga gggatgcacc attctgagat gtttttgttt
     2041 ggtccaaaca tgccgccttg ctgcttgata atattcattc tgacgagttc taacttggct
     2101 tcattgcacc gtttgacagg taactttgta atctcgctaa gttgagaatc ggtgattctg
     2161 tccattggtt tattccaccc ataggtttta cgcagaatgg caagcagcac tttaaactgt
     2221 cgcttggtca gatctgcgcc cgaataagcc tcaagcagca tatttgatag tctggcgtaa
     2281 ccatcatcga gatctgccac attacgctcc tgtccggcaa agttacctct gccgaagttg
     2341 agtatttttg ctgtatttgt cataatgact cctgttgata gatccagtaa tgacctcaga
     2401 actccatctg gatttgttca gaacgctcgg tcttgcacac cgggcgtttt ttattggtga
     2461 tttcatcaag cgcatactta aaagctctgc taatcggact gatgtctgat gccatgccaa
     2521 aagcacacaa gaccgaagct ataaacctcc agtctgttct gcttatcttc gattcatgac
     2581 agccaatcat ctttgccaga ccgcgctgag taagcgttga caggttgata agtaaatctg
     2641 tttctgcgcg atcaacgtcg cgctgtgata gtttgctgta atttgtttgt tccatttctt
     2701 actatttcca taggtaaata atcactaata cgcatctttc gatgagttct taattagtta
     2761 ccgcgttgtc ggcggtgcag attgataaag agcggtgtta cttatgctgc cagaaggttc
     2821 tttttgctta tttcaagcat ttcgcttgct tgatatttgc caccagaaat ctcttcgatt
     2881 tttgatgcgt atttcgtttt cccaaaaaac tcagtcttag ggaggaagcc gtttttgagc
     2941 cacttataga cagccctttc gctaactcca caagccttcg caacttcagg gatgccgaca
     3001 cctttaatcg gctcatcaag attttgcata ggaatgtcct ttttcgtact ttcagtacgc
     3061 attatggttg aactgaaagt ttttgcaagt gctttagtat cgtactcatg gttcagaatg
     3121 aaaaagtgcg caaagaattc gcccagcggc tagcgcaagc ctgtaaagaa gctggtcttg
     3181 atgaacatgg taggggaatg gccatagccc gtgccctttc tctttcgtcc aaaggcgtta
     3241 gcaaatggtt taatgctgag tctttaccac gccaggaaaa aatgaatgcg cttgcgaaat
     3301 ttctaaacgt tgatgttgtt tggcttcagc acggcacttc gttaaatgga gcgaatgatg
     3361 aagatacttt ttcaattgtt ggcaaattaa aaaaagggtt agtgcgcgtg gttggtgagg
     3421 caattcttgg tgttgatggt gccatcgaga tgaccgaaga gcgcgatggg tggctcaaga
     3481 tttatagcga tgatccagat gcctttggtc ttcgtgtaaa aggagacagc atgtggccca
     3541 gaataaaatc aggagaatat gtactcattg agcctaacac caaagtattc ccaggtgatg
     3601 aggtgtttgt cagaaccatt gaagggcaca acatgatcaa ggttcttggc tatgacagag
     3661 acggagaata ccaatttaca agcatcaacc aagaccacag gccaataacg ttgccttatc
     3721 atcaagtagc aaaggtggag tatgtagctg gtattctgaa gcaatctcgc cacctagatg
     3781 acatcgaggc cagggaatgg ctgaaaagtt catgacttca tcatcacata gctagtaacc
     3841 agtacgtttg gatggtggtg agaaacacac tgcaagcaaa cagaaacatg gatattaaaa
     3901 tattagctta acacagcaaa tcgaaagaaa cagcgagggc tcggatgtcc caagaattat
     3961 ctttaacctt taccgaaaac atatattatt caactaaaga acctgtaagc atcaaagatg
     4021 tgattacctc ccttcaaggg tgggaggcca tcgcaaaaca atcagaaggt gttttgcagg
     4081 aactgacagg ggctaatatc cttgatatat cggtgcatgt tgcgcgatta gaagcaggaa
     4141 gcctttatga agatattgtt attaaactcc tctttggtag ccaagaagaa atggacaagt
     4201 ttcttgctgg cgctcatgca aagatcggaa atgggaaaat gagaaacgct ctcgttggtg
     4261 ctgttgttat tggtctggtt ggatatggct tagttctagc aactaaggct atggctccaa
     4321 gcaacacctc ccactttgaa gcaaataaca atacaatcat caatattggt gccggtgagg
     4381 ctaatatttc acctgaccgt cttcaagcaa tcatagaaag cacggtaacc aataagaaga
     4441 ctcttgccaa aagttccatt aagacgcttg ccccagctag ggcagacgaa ggatctacga
     4501 tggtcatagg tacagggggt ggtacagtga ccattcccgc tgaaaccatt aaaaaagccc
     4561 caactgaagt tgtcttcaca ccagagtcat atactcagga tcattttgat gttgatgttg
     4621 aaatccgtgc attagacctc gacaatccag agaaaggttg ggctgctgtt atccctggat
     4681 taatagatcg aagagtaaat atggttcttg gccccaacgt aaacccatca gattttgcag
     4741 gtaaattttc tgtacgtgct gatataacaa taacgtacca gctcaaatca tcagataaaa
     4801 aataccaacc gaaagaagtc tttataaaag aaataatcaa ataattatac ccggcctcag
     4861 cgccggtttt tcttttcctc acgatcgtct accttattta acatccgcac atgtgctaac
     4921 ccacgaacta acacgccagc aaacaattct ttctctccta ctgaccaatc ttcaatcttt
     4981 acaaaaataa attcctttac atatcaaaaa catatctcat taagttaatg aaccacaaac
     5041 aattcgtact tatagttctt gataatatcg aactattggt tcattataat cgccatcagc
     5101 aggacgctgg cagccaaacg gaacagattg gcaggctctt taacttcgat gatgcgctga
     5161 caaagcgcga acagatacca aacgagatgg gtttggggtg atgtgaattg cagcagtaac
     5221 gacagcaacc agaagatcag catctggcgc atcaccacca aagccatttc acatgaggaa
     5281 aacatcatga cggtaatcgt gtacggaaaa tcaacatttg caggaaatgc caaaactcgc
     5341 cgtcatgagc ggcgcagaaa gctggccatc gagcgtgatt ccatctgcaa catcatcgat
     5401 tcgatcttcg gcacagacag tgaggaacct gttcaggaag acccgagaaa gcggttaagc
     5461 ctttctgaaa aagcaatatc actcggaagc cttcgctgca agaaagtaga tgaatgcagt
     5521 ggaagtgttt gcctgccaaa cgtagctatt tacgcggcag gctaccggaa atcaaaacaa
     5581 ctgacggcga gatgataaat tcatttgcta attacttgtt tttgccatgc ttatcctgag
     5641 cgataagttc atccataaga ctgtctttct tcccagcaaa cctaatgtag cactcatttc
     5701 tatagcgttc cgggataaca aaacggtcga tttcaggata tccagtagca gaaggtaccc
     5761 gaataagaag ccctttttcg agcaatgaga ttgcttcagg gcttcccttt tctgtcttta
     5821 gctggttatt agcggctaca gcgaatgcca aatacgctct ttctccaaga gttaacgaat
     5881 caaacaaatc ttgcacgtat ttctcttctt tagatttgcg cttctgagca gcgaatatct
     5941 caattcttac agtcacagcg tgataagcgg aattaacaac gccgttaagc acatagctaa
     6001 cgcaaaacaa caggatgtaa tacatccagt aatgaggaag gatttctgga ttatgcaggt
     6061 ttatccattc ttttacgctt acaggcataa caataatcaa tatgatcagg atgattagca
     6121 tatgaatcaa ctgtttaagt gtcattcctt gcaggaaaaa atgcattagt tcctgccacc
     6181 atgagttgtt catcggcgtt tctcttttgc tctctgtagg ggtgaataga gtttatccga
     6241 tttctcgctg taggggtaca cgagaaccac cgagcctgat gtggttaaaa gacaggcata
     6301 ctaataaaca ctgcactgtg tattcattcc aacgagtgaa tacactgagc aatgtcgctc
     6361 gtaactaaac aggagccgac ttgttctgat tattggaaat cttctttgcc ctccaatgtg
     6421 agggcgattt tttatctgtg aggatatgaa tagatgtcaa acatcaaaaa atacatcatt
     6481 gattacgact ggaaagcatc aatagaaatt gaaatcgacc atgacgtaat gacagaggaa
     6541 aaacttcacc agattaataa tttctggtca gactctgaat accgactcaa taaacacggc
     6601 tctgtattaa atgctgtatt aatcatgctg gcgcaacatg ctctgcttat agcaatttca
     6661 agcgacttaa atgcatatgg tgttgtgtgt gagttcgact ggaatgatgg aaatggtcag
     6721 gaaggatggc ctccaatgga tggtagcgaa ggaataagaa ttaccgatat cgatacatca
     6781 ggaatatttg attcagatga tatgactatc aaagccgcct gagcgcggcg ttaccgcata
     6841 ccaataacgc ttcactcgag gcgtttttcg ttatgtataa ataaggagca caccatgcaa
     6901 tatgccattg cagggtggcc tgttgctggc tgcccttccg aatctttact tgaacgaatc
     6961 acccgtaaat tacgtgacgg atggaaacgc cttatcgaca tacttaatca gccaggagtc
     7021 ccaaaaaatg gatcaaacac ttatggctat ccagactaaa ttcactatcg ccacttttat
     7081 tggcgatgaa aagatgtttc gtgaggccgt cgacgcttat aaaaaatgga tattaatact
     7141 gaaactgaga tcaagcaaaa gcattcacta accccctttc ctgttttcct aatcagcccg
     7201 gcatttcgcg ggcgatattt tcacagctat ttcaggagtt cagccatgaa cgcttattac
     7261 attcaggatc gtcttgaggc tcagagctgg gcgcgtcact accagcagat cgcccgtgaa
     7321 gagaaagagg cagaactggc agacgacatg gaaaaaggcc tgccccagca cctgtttgaa
     7381 tcgctatgca tcgatcattt gcaacgccac ggggccagca aaaaagccat tacccgtgcg
     7441 tttgatgacg atgttgagtt tcaggagcgc atggcagaac acatccggta catggttgaa
     7501 accattgctc accaccaggt tgatattgat tcagaggtat aaaacggatg agtacagcac
     7561 tcgcaacgct ggcagggaag ctggctgaac gtgtcggcat ggattctgtc gacccacagg
     7621 aactgatcac cactcttcgc cagacggcat ttaaaggtga tgccagcgat gcgcagttca
     7681 tcgcattgtt gatcgtcgcc aaccagtacg gccttaatcc gtggacgaaa gaaatttacg
     7741 ccttccctga taagcagaac ggcatcgttc cggtggtggg cgttgatggc tggtcccgca
     7801 tcatcaatga aaaccagcag tttgatggca tggactttga gcaggacaat gaatcctgta
     7861 catgccggat ttaccgcaag gaccgtaatc atccgatctg cgttaccgaa tggatggatg
     7921 aatgccgccg cgaaccattc aaaactcgcg aaggcagaga aatcacgggg ccgtggcagt
     7981 cgcatcccaa acggatgtta cggcataaag ccatgattca gtgtgcccgt ctcgccttcg
     8041 gatttgctgg tatctatgac aaggatgaag ccgagcgcat tgtcgaaaat accgcataca
     8101 ctgcagaacg tcagccggaa cgcgacatca ctccggttaa cgatgaaacc atgcaggaga
     8161 ttaacactct gctgatcgcc ctggataaaa catgggatga cgacttattg ccgctctgtt
     8221 cccagatatt tcgccgcgac attcgtgcat cgtcagaact gacacaggac gaagcagtga
     8281 aagctcttgg attcctgaaa caaaaagcca ctgaacagaa ggtggcagca tgacaccgga
     8341 cattatcctg cagcgtaccg ggatcgacgt gagagctatc gagcagggag atgatgcgtg
     8401 gcacaaatta cggctcggcg tcatcaccgc ttcacaagtt cacaacgtga tagcaaagcc
     8461 ccgctcagga aagaagtggc ctgacatgaa aatgtcctac ttccacaccc tgctggctga
     8521 ggtttgcacc ggtgtggctc cggaagttaa tgctaaggcg ctggcctggg gaaaacagta
     8581 cgagaacgac gccagaactc tgtttgaatt cacttccggc gtgaatgtta ctgaatcccc
     8641 gatcatctat cgcgacgaaa gtatgcgcac cgcctgctct cccgatggtt tatgcagtga
     8701 cggcaatggc cttgagctga aatgcccgtt tacctcccgg gatttcatga agttccggct
     8761 cggtggtttc gaggccataa agtcggctta catggcccag gtgcagtaca gcatgtgggt
     8821 gacacgaaaa gatgcctggt actttgccaa ctatgacccg cgtatgaagc gtgaaggact
     8881 gcattatgtc gtggttgagc gggatgaaaa gtacatggcg agttttgacg agatggtgcc
     8941 ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt ggttttgtat ttggggagca
     9001 atggcgatga cgcatcctca cgataatatc cgggtaggcg cgatcacttt cgtctactcc
     9061 gttacaaagc gaggctgggt atttcccggc ctttctgtta tccaaaatcc actgaaagcc
     9121 cagcggctgg ctgaggagat aaataataaa cgaggggctg tatgcacaaa gcatctcctg
     9181 ttgagttaag aacgagcatt gagatggcac atagccttgc tcaaattgga gtcaggtttg
     9241 tgccaatacc agcagaaaca gacgaagaat ttcatacgtt agccgcatcc ctttcacaaa
     9301 agctggaaat gatggcggcg aaagcagagg caaacgagag agagcttgca tgacaacaac
     9361 agaatgcatt tttctggcgg cgggcttcat attctgtgtg cttatgcttt ccggcatggg
     9421 gcttgttcag tgacacctca gcagaaaaac gcccttcgca gcattgcccg tcaagctaac
     9481 tctgaaatca aaaaagccag acagcagttt ccggataaaa acgtcgatga catttgccgt
     9541 agcgtactga agaagcaccg cgaaacggta acgctgatgg gattcacacc gactcattta
     9601 agcctggcga tcggcatgtt aaacggcgtc tttaaggagc gatgaacatg aaaagcaaaa
     9661 tcatcaggga gctacaggct ccttttttat tattcgcatt caccctcaag cgtattaacc
     9721 aacaattcag ggattaatgg aagatggcag acatcattga ttcagcatca gaaatcgaag
     9781 aattacagcg caatacagca ataaaaatgc gtcgtctgaa ctaccagact gtatctgcca
     9841 ctcattgttg tgagtgtggc gatccgatag atgagcgaag acgcctggtc gttcagggtt
     9901 gtcggacttg tgcaagttgc caggaggaga tcgaacttaa gaacaaacaa tggggattgt
     9961 gatggcctca aagcagcaaa tttcaacatc gtccaactga ggtgtaaaaa tgctcagaat
    10021 catttttcct aacacctggt acgtcgacca ccacggcact ccctgcaaaa tcctgcgttc
    10081 tacctacaac aaagttcact acatccgaaa aggcagaaca tgtatcgcca gcatgttccg
    10141 ctttaatcat gactttgaac ctgtgaataa agctgatgca gatcggatag cagaagagat
    10201 cgaaacggca gaacacatta agaagttacg tgccatacgc aggaaataga aaaattgata
    10261 aattcaatac tgcatttctc agcattaaat ttatctctat gaccagtcaa gagatgtacc
    10321 tgccatgagc ttaatatcat gtcagatata tcggtcacaa actccctcag cagctaagag
    10381 gaggacaaat gtctcgacta atcactttac aggactgggc taaagaagaa tttggggact
    10441 tagcaccaag tgagcgagtt ctgaaaaaat acgcgcaagg gaaaatgatg gccccacccg
    10501 ctataaaagt tggtcgctac tggatgattg accgaaattc ccgttttgta ggaacgcttg
    10561 cagaaccgca actcccaata aacgcaaacc caaaactcca acggataatc gctgatggct
    10621 gctagacccc gatctcacaa aatctctata cccaatttat attgcaaatt agataagcga
    10681 accggaaagg tatattggca atacaaacat ccactatccg gtcgttttca tagcttagga
    10741 actgatgaga atgaagcaaa acaagttgct actgaagcaa ataccattat tgctgaacaa
    10801 cgtacccgac aaatattaag cgtcaatgag cgtctggaaa gaatgaaagg caggcgctca
    10861 gaaattacgg tgacagaatg gcttgataaa tatatttcta tccaggagga caggctgcaa
    10921 cataatgaac taagacccaa ctcctatcgg caaaaaggca aacccattcg tcttttccgt
    10981 gagcattgtg gaatgcaaca cctcaaggat attaccgcac ttgatattgc cgaaataatt
    11041 gatgctgtaa aggctgaagg tcataacagg atggcgcaag tcgtgagaat ggtgttgatc
    11101 gacgtcttca aagaagcaca acacgcagga catgttccgc caggatttaa cccagcgcag
    11161 gcaacaaaac aaccgcgaaa tcgagtaaac cgccaaagat tgtcactgcc cgaatggcag
    11221 gcaatatttg aaagcgtaag cagacggcag ccctatttaa aatgcggcat gctacttgct
    11281 cttgttactg gacaacgttt aggtgatatc tgcaatttga aattctctga tatctgggac
    11341 gacatgttgc acattactca ggaaaaaacc ggttcaaaac ttgctattcc gcttaacctg
    11401 aaatgcgatg ctctgaatat tacccttcgt gaagttatat ctcagtgcag ggatgctgtt
    11461 gttagtaaat atctggtcca ttaccgtcac actacctctc aagcaaacag aggagaccag
    11521 gtttctgcaa atactctgac aacggctttt aaaaaggcca gggaaaaatg tggcataaaa
    11581 tgggagcaag gaactgcgcc cacatttcat gagcagcgat ctctgtcaga acggttatat
    11641 cgggaacagg gtctggatac gcaaaagttg ttaggccata aatccagaaa aatgaccgac
    11701 cgatacaatg atgatcgtgg taaagactgg gttatcgtag atatcaaaac agcatagaaa
    11761 atagccagtt ttggggaagg gttttgggga aagttttggg gaagatttta catcatcata
    11821 aaacaacggg cgtataacac gcccgtttaa atatttaaca catgtagtga ttacatgttc
    11881 ttgatgatcg cgtcgccaaa ctcgctacat ttcagcaatt tagcgccttc catcagacgt
    11941 tcgaagtcat aggttac
//
LOCUS       DAYPHN010000145        11746 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000145 DAYPHN010000000
VERSION     DAYPHN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.145"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            129..344
                     /gene="bdm"
                     /locus_tag="ACG0BW_003765"
     CDS             129..344
                     /gene="bdm"
                     /locus_tag="ACG0BW_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFK7260675.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            446..583
                     /gene="sra"
                     /locus_tag="ACG0BW_003766"
     CDS             446..583
                     /gene="sra"
                     /locus_tag="ACG0BW_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFK7260676.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            740..2437
                     /gene="maeA"
                     /locus_tag="ACG0BW_003767"
     CDS             740..2437
                     /gene="maeA"
                     /locus_tag="ACG0BW_003767"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFK7260677.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2571..3581
                     /gene="adhP"
                     /locus_tag="ACG0BW_003768"
     CDS             2571..3581
                     /gene="adhP"
                     /locus_tag="ACG0BW_003768"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFK7260678.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3767..4045
                     /locus_tag="ACG0BW_003769"
     CDS             3767..4045
                     /locus_tag="ACG0BW_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFK7260679.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            4045..4329
                     /locus_tag="ACG0BW_003770"
     CDS             4045..4329
                     /locus_tag="ACG0BW_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFK7260680.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4557..5210)
                     /gene="fdnI"
                     /locus_tag="ACG0BW_003771"
     CDS             complement(4557..5210)
                     /gene="fdnI"
                     /locus_tag="ACG0BW_003771"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFK7260681.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5203..6087)
                     /gene="fdnH"
                     /locus_tag="ACG0BW_003772"
     CDS             complement(5203..6087)
                     /gene="fdnH"
                     /locus_tag="ACG0BW_003772"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFK7260682.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCIGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(6100..9147)
                     /gene="fdnG"
                     /locus_tag="ACG0BW_003773"
     CDS             complement(6100..9147)
                     /gene="fdnG"
                     /locus_tag="ACG0BW_003773"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8560..8562),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFK7260683.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWDEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9379..10260
                     /gene="yddG"
                     /locus_tag="ACG0BW_003774"
     CDS             9379..10260
                     /gene="yddG"
                     /locus_tag="ACG0BW_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFK7260684.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGCLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10519..11619
                     /gene="ompC"
                     /locus_tag="ACG0BW_003775"
     CDS             10519..11619
                     /gene="ompC"
                     /locus_tag="ACG0BW_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFK7260685.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNGHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2898 a         3000 c         2910 g         2938 t
ORIGIN      
        1 ataatataaa aattatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
       61 gctgaatgtg ctttatatat ctgcgatccg tagcagacac caaaaataca cagacacgga
      121 gaatcgctat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
      181 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
      241 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
      301 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
      361 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
      421 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
      481 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt
      541 ggtaaataac ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
      601 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata
      661 cttggggccg acgccctggc ggtaaagcaa agacgataaa agcgtgccag ggatggatat
      721 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatattcc
      781 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagcg ccttcagcat
      841 ggaagaacgc cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga
      901 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
      961 catctacctg cgtaacatcc aggacactaa cgaaaccctc ttctaccgtc tggtaaacaa
     1021 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
     1081 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accgccacaa
     1141 tatggacgat attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga
     1201 cggcgaacgt attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
     1261 taaactgtcg ctctataccg cttgtggcgg catcagcccg gcgtataccc ttccggtggt
     1321 gctggatgtc ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg
     1381 taatccgcgt atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt
     1441 gaaacaacgc tggccggacg tgctgttgca gtttgaagac ttcgcacaaa aaaatgcgat
     1501 gccgttactt aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac
     1561 cgcggcggta acggtcggca cactgatcgc agccagccgc gcggcgggtg gtcaactgag
     1621 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
     1681 catcgcccag acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat
     1741 ggtcgatcgc tttggcctgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa
     1801 actggtgcag aaacgcgaaa acctcagtga ctgggatacc gacagcgatg tgctgtcact
     1861 gctggatgtg gtgcgtaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
     1921 gctgtttacg gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat
     1981 gccgctgtct aacccgactt cacgcgtgga agccacaccg caggacatta tcgcctggac
     2041 tgaaggtaac gcgctggtcg ccactggcag tccgtttaac ccagtggtat ggaaagataa
     2101 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccgggcatcg ggctgggcgt
     2161 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct
     2221 tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga
     2281 tattcagaaa gtctcccgcg caattgcctt tgcggttggc aaaatggcgc agcagcaagg
     2341 cgtggcggtg aaaacctccg ccgaagctct gcaacaggcc attgatgata atttctggca
     2401 ggccgaatac cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct
     2461 accgggcaat ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa
     2521 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg
     2581 cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac
     2641 atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcacaccgat cttcatgtta
     2701 agaatggcga ttttggtgac aaaaccggcg taattctggg ccatgaaggc atcggtgtgg
     2761 tggcagaagt tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
     2821 ggttttacga aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc
     2881 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
     2941 tcgccgatta cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta
     3001 cctgtgcggg ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt ccagggcagt
     3061 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg
     3121 tctttaacgc caaagtgatc gccattgatg tcaatgatga gcagttgaaa ctggcaaccg
     3181 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
     3241 agaaaaccgg tggtgcacac gctgcggtgg tgacagcggt agctaaagct gcgtttaact
     3301 ctgcggtcga tgccgttcgt gcaggcgggc gtgttgtggc agtcggtctg ccgccggagt
     3361 ctatgagcct ggatatccca cggctggtgc tggatggtat tgaagtggtc ggttcgctgg
     3421 tcggcacgcg ccaggatctc accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
     3481 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttactgag atggaagaag
     3541 gcaaaatccg tggccgtatg gtgattgatt tccgccgcta agaagccttt gctgcgactg
     3601 ccatgttcgg gtcgcagcct gttttgcgag acgctttccg caatttcact cttcttcttt
     3661 tcctccccca tctttcccct cacaattcgc gaaaacgtag cgaaatttgt tgcgctgcac
     3721 ggaacaatag tgatagtgtt cctttcgagg gaacaaggat gaagagatga taatgaattt
     3781 cagacacaag ggattgcgtg acttgtttct tctcggcaaa acctccggcg ttataccgac
     3841 gcaagtcaaa cgattacgcc atcgacttgc tgtgattgat gcggcaagct gtcttgctga
     3901 tatcgatatg cccggttacc gactacaccc gttaagcggc gatcgcgatg gaatttgggc
     3961 gatatctgtc tcgggcaact ggcgaatcac atttgaattc gtcaatggcg atgcatacat
     4021 actggattac gaggactatc actgatgaaa atggccaatc atccccgccc gggggacatt
     4081 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     4141 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     4201 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     4261 gcctggagtc tggcagaggc tgaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     4321 acgcaataaa aaaatggcac tttataaagc gccatttaga actgatgaca aacgcaacat
     4381 tgcctgatgc gctacgctta tcaggcctac aagttcactg caatctattg gatttgaagg
     4441 gtttgtaggc cggataaggc gtcacgccgc atccggcatg aacaaagcct acgttgtcta
     4501 caatatgaaa atgacgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4561 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgtgga
     4621 tggtgtttct tcgcccagcg acggctcacc tttccttcga tcatcccttt aatcgatcct
     4681 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4741 gccgcgtgga tcagcaggct ataacggaca acctgtatcg ggaagtactg cgcaaagtac
     4801 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4861 aacatcatct tttgcccggc gttgtactta ccgacatccg ccactttgtg ctcattgcct
     4921 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4981 tgcacaaaac gcacaaacat aaacatcagc gcgacgaaaa tcgcaatgcc gaagaacggg
     5041 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     5101 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     5161 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     5221 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     5281 aaaagtggca ataaagccag ccgctgccag cggtttcagc gcgcctttcc acaggcttac
     5341 cgaggtgtcg atcttcggat ctttcggcag accgtgatac agctccggct gatcggcgtg
     5401 atgcagcacg tacataacgt gcgtaccacc gaccccttcc gggttgtaga cgccagcatg
     5461 ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc gccagctcca gcatctcctt
     5521 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5581 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5641 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5701 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5761 caggcagccc ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5821 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5881 ggggttatcg taaaccccga tgcagtgccc cacttcatca cggatgtcgt tccactccga
     5941 acacgccacc tgacaggctt tacaaccgat acaggtggaa acgtcgataa gtttcgcgac
     6001 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     6061 gataatgtcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     6121 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     6181 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     6241 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     6301 cgcgcggata aatccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     6361 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     6421 caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6481 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6541 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcga ttggctcgta
     6601 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6661 cggctgcatg ataaacggtc cggttggtgt acctggcgcg gcattgccga agtccggaat
     6721 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6781 tttaccgttg atatccgccg aagcacggtt gtagagtacg cgacggttga gcggccacgc
     6841 ccaggcccat cccagcgtat tccccagacc ggacgggtcg gagttatcgc ggttagccat
     6901 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6961 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     7021 attggcatca tagagatctt ccagcgcgta gccgttgttc tctttagcta cttcgtcaga
     7081 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     7141 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     7201 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     7261 ggagttagca atagaaccat cttcttcagc aaagcaggtc gaaggcagac ggaatacttc
     7321 agtctgaatg gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     7381 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     7441 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga aataaccggt
     7501 gactttgcct tcatccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7561 cggcagccag tcatagcccc agttgttctc tttctgtgca gcatcaccgt agaaagattt
     7621 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7681 cgctttcggc gtattcgctt ccagatacga ctgcaaatca acctgttttt ctgacggcag
     7741 cgtcagataa cccggcaggc tggttgagag cagacctaag tcggtcaggc cctgaatgtt
     7801 ggagtgacca cgcaatgcgt tcacgccgcc accagccata cccatgttac cgagcagcaa
     7861 ttggatcatc gccatggtgc ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7921 cgcgtacagg aaggtggttg tgcgatccgg cgcgctggtg gaggccagca cttcacacac
     7981 tttcaggaag tctgcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     8041 ggaaacgtgc tctttcagca agttccacac acagcgcgga tgagtcagtg tttcatcgcg
     8101 tttcgcatag ccgttttcat cgaactgata gttccaggac gatttatcgt actggcgttt
     8161 ttcagcgtca taaccgctga acaggccatc ttcgaaagca aaatcatcac gcaccagcag
     8221 gctggcgttg gtgtaatgct taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     8281 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     8341 cgccacagaa gcggtacgcg taaaacgggg atcgacaacg atcaaggttg catcgttgtt
     8401 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     8461 catcaccatc acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8521 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8581 taccgccagc atcccgaggg agcgggcaaa tttttgagtc agcatcccgg tttcgttgct
     8641 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8701 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8761 tgcttcatcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8821 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gcccctttcg ggcacagcgc
     8881 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8941 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     9001 ggtgttacgg atctctttcg cgcgtaataa tttatagttt cgcgcctgag ccagtgcttg
     9061 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     9121 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     9181 gaggaaacta acagcaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgttt
     9241 gccacaaaat aatccgcctg cgcaaattga cctacctcaa tagcggtaga aaaacgcacc
     9301 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     9361 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     9421 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gtgaggggct cggcccggtc
     9481 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9541 ccgcgtattc ggcagatccc gaaaggctat ttactcgccg ggtgtctgtt attcgtcagc
     9601 tatgaaatct gtctggcgct ttccttagga tatgcggcga cccgtcatca ggcgattgaa
     9661 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9721 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt tggcgtctgc
     9781 tgggtgttag gtggtgacaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9841 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctactgcaca
     9901 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9961 gcaagtctgt gggtttacta ttttctcacg ccacaaccag aaatggtatt tagcacgcca
    10021 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaac
    10081 gtcggtatat tacatggcaa cgtcaccatt atggcggtag gttcgtattt tacgcccgtc
    10141 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
    10201 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggctaa
    10261 aataattgtc gggtctacgg cccggcagtt aatcagataa attccgacaa tataaaaaaa
    10321 taaatttcaa ataccagtag ttcaccttat atttcatttc gacttcgatc acatatcgat
    10381 aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta ttttaaaaac
    10441 agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa
    10501 ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta
    10561 gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt
    10621 tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa
    10681 acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt
    10741 ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggtaac
    10801 aagacccgtc tggcctatgc aggtttaaaa attagtgaat ttggctctct ggactatggt
    10861 cgtaactacg gtgtcggcta tgacattggt tcatggaccg atatgttgcc agaatttggt
    10921 ggcgatacct ggagtcagaa agatgtcttc atgacatacc gtactaccgg tgtggcaacc
    10981 tatcgcaact acgatttctt cggcttaatt gaaggtctga actttgccgc gcaatatcaa
    11041 ggcaaaaatg aacgcactga caatggtcat ctttatggtg ctgactacac gcgtgccaat
    11101 ggtgacggtt tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggtg
    11161 tataccaaat ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc
    11221 tccggtaaga atgcagaact gtgggctaca ggtataaaat atgatgccaa caacatctac
    11281 tttgcagcta attacgctga aacattaaac atgaccacct atggcgatgg ttatatctct
    11341 aacaaagcac aaagttttga agtagtggca caatatcaat tcgacttcgg cttgcgccca
    11401 tcactcgctt acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg
    11461 attgagtata tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt
    11521 gattataaaa tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcgcacc
    11581 gataacatcg tcgcaacggg cattacctat cagttctaac atcctctcct gaccagccct
    11641 tctctgggct ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa
    11701 tttttgatat ttctggtcat tcacctcaat taatattatt ccatac
//
LOCUS       DAYPHN010000146        11667 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000146 DAYPHN010000000
VERSION     DAYPHN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.146"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            287..1585
                     /gene="kgtP"
                     /locus_tag="ACG0BW_003776"
     CDS             287..1585
                     /gene="kgtP"
                     /locus_tag="ACG0BW_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFK7260686.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1582..1905)
                     /gene="yfiM"
                     /locus_tag="ACG0BW_003777"
     CDS             complement(1582..1905)
                     /gene="yfiM"
                     /locus_tag="ACG0BW_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFK7260687.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTIW
                     QLAHH"
     gene            complement(1951..3306)
                     /gene="pssA"
                     /locus_tag="ACG0BW_003778"
     CDS             complement(1951..3306)
                     /gene="pssA"
                     /locus_tag="ACG0BW_003778"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFK7260688.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3420..6080)
                     /gene="pat"
                     /locus_tag="ACG0BW_003779"
     CDS             complement(3420..6080)
                     /gene="pat"
                     /locus_tag="ACG0BW_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFK7260689.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNANRNLVLLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEYSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEYDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6112..6810)
                     /gene="tapT"
                     /locus_tag="ACG0BW_003780"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6112..6810)
                     /gene="tapT"
                     /locus_tag="ACG0BW_003780"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFK7260690.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6879..7298)
                     /gene="trxC"
                     /locus_tag="ACG0BW_003781"
     CDS             complement(6879..7298)
                     /gene="trxC"
                     /locus_tag="ACG0BW_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFK7260691.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     FDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7505..8542
                     /gene="yfiF"
                     /locus_tag="ACG0BW_003782"
     CDS             7505..8542
                     /gene="yfiF"
                     /locus_tag="ACG0BW_003782"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFK7260692.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8590..9279)
                     /gene="ung"
                     /locus_tag="ACG0BW_003783"
     CDS             complement(8590..9279)
                     /gene="ung"
                     /locus_tag="ACG0BW_003783"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFK7260693.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            9584..9967
                     /gene="grcA"
                     /locus_tag="ACG0BW_003784"
     CDS             9584..9967
                     /gene="grcA"
                     /locus_tag="ACG0BW_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFK7260694.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10021..10608)
                     /gene="eamB"
                     /locus_tag="ACG0BW_003785"
     CDS             complement(10021..10608)
                     /gene="eamB"
                     /locus_tag="ACG0BW_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFK7260695.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10711..11592
                     /gene="yfiE"
                     /locus_tag="ACG0BW_003786"
                     /pseudo
     CDS             10711..11592
                     /gene="yfiE"
                     /locus_tag="ACG0BW_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         2895 a         2921 c         3014 g         2837 t
ORIGIN      
        1 cgtcttaccc gacctacgaa gagttgcggc gagtgtaaat atccgcatta tccccccaca
       61 taagccagat tcaacagcga atacggcttc cccaaattgc ccacttccat acttcctcct
      121 taccagaaat ctatccttaa gctccttaat aaccattttc ctgctaacta aattcatggt
      181 taaggttgca taatgatatg caacaaatgt ataacatttt ctttgccaaa aaaataaaca
      241 acagcgaccg acaaaagcat cggattacgg caggagacat aatggcatgg ctgaaagtac
      301 tgtaacggca gacagcaaac tgacaagtag tgatactcgt cgccgcattt gggcaattgt
      361 gggggcctct tcaggtaatc tggtcgagtg gttcgatttc tatgtctact cgttctgttc
      421 actctacttc gcccacatct tcttcccttc cgggaacacg acgactcaac tactgcaaac
      481 agcaggtgtt tttgctgcgg gatttctgat gcgcccgata ggcggttggc tatttggccg
      541 catagcagat aaacatggtc gcaaaaaatc gatgctgtta tcggtgtgta tgatgtgttt
      601 cggctcactg gttatcgcct gtttgccagg ctacgaaaca atcggtacct gggctccggc
      661 attattgctt cttgctcgtt tattccaggg attatccgtt ggcggagaat atggcaccag
      721 cgccacctat atgagtgaag ttgccgttga agggcgcaaa ggtttttacg catcatttca
      781 gtatgtgacg ttgatcggcg ggcaactgct agctctactg gttgtcgtgg ttttacaaca
      841 caccatggaa gacgctgcac ttagagagtg gggatggcgt attcctttcg cgttaggagc
      901 tgtgttagct gttgtggcgt tgtggttacg tcgtcagtta gatgaaactt cacaacaaga
      961 aacacgcgct ttaaaagaag ctggatctct gaaaggatta tggcgcaatc gccgtgcatt
     1021 catcatggtt ctcggtttta ccgctgcggg ctccctttgt ttctatacct tcaccactta
     1081 tatgcagaag tatctggtaa atactgcggg aatgcatgcc aacgtggcga gtggcattat
     1141 gactgccgca ttgtttgtat tcatgcttat tcaaccactt attggcgcgc tgtcggataa
     1201 gattggtcgt cgtacctcaa tgttatgttt cggttctctg gcaaccattt ttaccgttcc
     1261 tattctctca gcattgcaga acgtttcttc gccttatgcc gcttttggtc tggtgatgtg
     1321 cgccctgctg atagtgagtt tttatacttc aatcagtgga atactgaagg ctgaaatgtt
     1381 cccggcccag gttcgcgcat taggtgttgg tctgtcgtat gccgtcgcta atgcgatatt
     1441 tggtggctca gcagagtacg tagcgttgtc actgaaatca ataggaatgg aaacagcctt
     1501 cttctggtat gtgaccttga tggccgtggt ggcgtttctg gtttcactaa cattgcaccg
     1561 taaaggaaaa gggatgcgtc tttagtgatg cgccagttgc cagatggtat agccggtagt
     1621 tgcaccggcg acatcccagg taaaatcttt ccagctccag ccgctcccct cggggcggct
     1681 atcccaaagt tcttttgacg cgcccagccc aacagagaac atcagcccga aagtggcact
     1741 gcgatcgcga ctcatcccct gatgctgtgc atattcattt ccggcggcgg aaagcatcgc
     1801 cgaggcgata aagtgttgag ctttatcctg cccgctccag ctatcgttag ccatatggct
     1861 gcatccagaa agtaacaata gcgagcagac aaaaagaata cgcaaaacgt ggcctccaaa
     1921 aaacaaaccc cgtacaggac ggggttgtga ttacaggatg cggctaatta atcggtcgat
     1981 gcggatacgg cgcaaacgac ggatgagttt acgaaccttc accggataat cggcaatact
     2041 ttgcagatcg cgatagtgct taacgatggt ggtatgctcg cggatcagct ccagttcttt
     2101 ctctcgctgt ggcgcaagct ccagttgcgg atcgtggatc aaaatggcgt tttccagatc
     2161 cagacgccag gcgcgcgggt tcaggttatt accggtgatc aacatccact tatcatcaac
     2221 ccacatccct ttcaggtgat aggtgttgtc gtcatctttc cataaccgaa ccactaactg
     2281 gtcagtattg acgtaatact gcaaacggct caggaaacga cgcagattga tctcatagag
     2341 ataaggcaat gcgccaatta tcttgaaagg ttcatcttcc ggaatgtaga agtcattcgc
     2401 ggttttatca ccaacaataa tttcgacctt tttcccttcg cgcagtaact ggatgatatt
     2461 gcgcacaagg attgctggca ggttgaagta tggtgtgcag atggttagtt tctgctcggc
     2521 acaaggcata agatggaaaa tggtcttgtt caacagactc gatttcccca gacctaccaa
     2581 cggcgttacg gaaagctgat cgttgtcggc atcgccctgg aaatgataag cggcatcacg
     2641 cagctcctgg cggaacagac gaatatcgtt cttgatttcc gggctttttg gccgattaac
     2701 atcatccaga cgattaacgc cgcggccatt cataatattc tgtgtaaccc attcaaacat
     2761 aatgtctgac atcttacggt tacggataag atgataacgg tcgtagcgat atttatcgtg
     2821 ctgatgcagg taaacatcgt tcaggctggc accgctataa agtacgctat cgtcgatgat
     2881 aaagccttta aagtgcagaa caccaagggc ttcacgggta ttgattggaa cgccataaac
     2941 cggaacatct acgcccggat tttcctgcgc catgcggcag taccagtcag cgttagtgtt
     3001 agatgccgca gcgccaatgc gtccacgttg tgcacgatgc cagtcgacca gcacccgcac
     3061 atccagttcc ggacgctgcc ttttagcctc atacaacgcg ttcagaatgc ctttgccacc
     3121 gtcatcctgt tcgagataca gggcgacaat gcaaatgcgc tgcttcgcgc tggctatttt
     3181 ttccagcagc gtctcccgga agtcggcggg agcgtaaaag aaatcgacat catcaactga
     3241 ttgagaaatc ttgggtagtt gggcaaggtg ttgttgatgt ttattacgct taaattttga
     3301 caacatcaca gtgcatttct tctctgttca ttgaagggtc ctctgtgcaa tgcagacgac
     3361 ataagcgggc aataataaca ccagtcccga ttaagtggtc aacatttcca gtaccttact
     3421 catgattcct cgcgctgggc aagatttagc gtaagcccaa cgatcccctc ttcgagctgg
     3481 atatcaacgt taaacccgag cttgcgggcc agcgccacca tgccacgatt gtttggcatc
     3541 gtaataccat tcagacgttg cagtccgtga tctcgcgtat aggtaatcaa cttttccatt
     3601 aagcgtcgac ctaagcctaa ccctttgaga tccgagcgaa ccagcacggc aaattcggca
     3661 tcgatgttat cagggtcgga gatcgcacgc gtgacgccga ggatctcttc cgtttgatca
     3721 atacgtcgta ccgctacaaa cgccatttcc cgatcgtagt cgatctgcgt catgttggct
     3781 aaatcttcat gggtaaattc gttgatctcg ctaaagtagc ggtaataaag atcttctttg
     3841 gtgactcgcg aaatgaactg ctgaagttgt ggctcatctt ctggcaaaat cgggcggaac
     3901 aagcagcgtt caccgttttt caattctacc cactcttcca gctgatgtgg ataagggcgc
     3961 actgccagcc ggctctcgtt atcgccttca aacggcgcga tatccagcgt gacgtccagc
     4021 gcggtaaatt cactgccaga agccagcaaa ggatgaatgt ccagacgttg aatttccggg
     4081 caatcgacaa tcaagttgga aacctgcacc agaagctggc tcaagcctgc aacatccaat
     4141 gggcgtagcg cactgcgcgc acgaatcttt ttacttttga tcccctgaat aaccagatag
     4201 cgggccaggt tcatgttcag cggtggtaac gcgacgacgg cttgatcttc aggacgccac
     4261 tccacaccgc cttcgcccag catgatcaac ggcccgaaaa ccggatcgta ctcaaccaca
     4321 acccgcaact cctgagcgcc agcacggtta gccatacttt gcactaacag gccgtggatc
     4381 cgcgcctgcg gccaggccat ttttacgcga tcgaaaatag cgtttgccgc ttgctggact
     4441 tcattagctg tacgcaggta gagcatgacg ccctgaactt ccgatttatg tggaatatcc
     4501 ggcgaacgca atttcagcgc caccggataa ccaatctgtt cggcaatatg caccgcttcg
     4561 gtgctatcgc tggcaatcca ggtggggagc gtgttcatgc catacgcttg caggatgggc
     4621 tgaacctcat gggtatcaag cgacgtagcc ccttcggcaa tcgcctgttg caacagaaga
     4681 tgcgcttctg cggtattgga ggtcagattg ctgggcaacg ccggcgtttc gcgtagttgc
     4741 ttctgattac gccggtactc caccatatgc ataaaagcag tgatggttcc ttccggcgtg
     4801 cgataggtcg gcagcccggc ttcactgaat aatcgtcgtg cctcttgcga ggagtactcg
     4861 ccacaccagt tcgtcagcag agaaacatac ttgctgcggg gatgatgctt taccgcttca
     4921 attaatgctt gcgcactttc tgttgcggga gcagcggcgc tgggcgaatg aataaccatc
     4981 agcgcgtcaa aatcctggct gtggagcaga atatccagcg ttttaatata gtgctcactg
     5041 ctggcgtcat cgcgtagatc gagcgggtta gagattgcca catgttctgg cagtgcatcg
     5101 cgcagtttct ggcaggtttc ttcgcttagc gttgccagct tgccattgcg tgaccataag
     5161 gcatccagcg ccagcgcggc aggcgcagca ccgttgctga taatcatcag ccggtcgcca
     5221 cgcagcgggc gcatatggct gagggtttcc accgccgaaa acagctcgtg agtatcctgt
     5281 acccgcaaca aaccggcacg ctgaatagcc gcatcccacg ccggatccat tcctgccgtg
     5341 gtgttcaaca gacgttgagc cgccgggcta cgtccgcttt taatcaccag aatcggttta
     5401 ttgcgagagg cactacgggc cgccgaaaca aagcgtcgtg cgtcgcttaa ctgttcgaga
     5461 tagagcagga tggcgctggt tttactgtcg cgcgccagat agtcgagcaa ttcatcaacg
     5521 tcgatatcca ggctgtcgcc gagcgcaata aagtaggaaa agcccatctc acgctgttgc
     5581 gcccagtcga ggatggtgtt ggagacagca gccgattgcg aaataaacgc cagcttgccg
     5641 cgtttaatcg gcacaggcga aaagctggca ttcagacctt gccagggagc cagtaagccc
     5701 agactgtttg gtcctagcag gcgcatgtta tggcgcaggg cgcaggcgcg gagatcttca
     5761 tgttgcgatg ccggggcgga aagaataatg caggttttac agcctttctc gccgagctct
     5821 tccagaagaa caagattacg gttggcatta gtacataaaa ccgcaaggtc gggtgtaaag
     5881 ggcaagctgg caatatccgg ccaggccaac acacccaaca ccgctttcca ggctggcgtc
     5941 accgggagta ccggtccgtt aaagcctccc gccagcaggt tacgcatcat caggtaacct
     6001 gcgcgattgg gtttcatcga tgcgccaatt accgcaatcg attttggtcg cagtagtgct
     6061 tccagtcctc gctgactcat accggtctcc cgcagaagtg accgaatgat tttaaacgct
     6121 ttctaactgt tctgctgtga tgctacccag atgttgcgtt tttcctgcca gatagcgtgt
     6181 tttaaagcgg gtaaaatgct cgcctaaccc tgccgccgcc ccggtatcgc cggccatatc
     6241 taacagtgcg atggctactt cggcagtaca atattggcct tcagcctggg cttcacgcag
     6301 gcgataggca gaaagccggg aaagatcgac ggaaatgacg ggaagattat ccagatacgg
     6361 acttttacga aacatcttgc gagcttctgg ccaggtgcca tcgagcatga taaacagcgg
     6421 tggcttaccg gcaggtggtg tgaagatcac ttcccgttgc tcgtcagcat acgaggcggg
     6481 aaagaccacc attggttgat agtccgggtt ttgcaccaga tccagcaaat cttgcgaggg
     6541 ttcggtacgc gaccattgaa acgcaacggt atcaggcaaa atatcagcaa tcagacgccc
     6601 ggtattactg ggcttcattg gctcggtgtc gaacatcagc aaacaaaagc gactttttgc
     6661 ttgtgctggg gtaattgtcg aacagagaca taatttctct ggcaaaagac agcgttggca
     6721 gcgacgaacg cgatttccgc gggcaagaaa aggacgtgtt gcgcgcgcaa tacgctcggc
     6781 gcgtaactgg agaacagcgt tttcggtcat aagagagtgt cgaaaaaacg ccattgtcgc
     6841 agaggagaaa acggggcaca agatgcgccc cggtaagatt aaagagattc gttcagccag
     6901 ctgtcgaacg gcgctttcgg tactgcgcca ttaagcatgt cgacaacctg accgtttttg
     6961 aaaatcatga tcgtcggtat gctacgaatt ccaaagcggc tgcttaattc acgttcagct
     7021 tcggtattca ctttcacaaa gcgcacttta ccgctacgct cttgcgcgac atcttcaaaa
     7081 attggtgcga aattacggca gggaccgcac cacggtgccc agaagtcgat caccacaggt
     7141 agatcatctt tcagcaattt gtcgaacgtt tcaccggtcg cattaataac ctctccgtca
     7201 aacaagtcgt gaccgcagcg tccgcatttt gccgcatctt cgatccgatc gtcgggaatg
     7261 cgattgatgg cctgacaatg ggtacaaacg gtattcataa ctaacctcgg gatgagtaag
     7321 gagacttatc ggcaacgacg ccggtatgtt tctaatatgt tacatattat cggggacatt
     7381 gttccagaca acaatctgtt ttattcaata ggtatgatag tcgcaggctt tggtacgaaa
     7441 tcatggcgaa ccgcgcgcac ttcgggtaat ctgcgcgctt cgcgcagcgc tggtggagaa
     7501 aagcatgaac gatgaaatga aaggtaaaag cggcaaggtc aaagtgatgt atgtccgcag
     7561 tgatgatgat tctgataaac gtacccacaa cccgcgtacc gggaaagggg gcgggcgtcc
     7621 aggaaaatct cgtgctgacg gtggccgtcg ccccgcccgc gatgacaaac aaagtcagcc
     7681 ccgtgaccgc aagtgggaag agtcgccctg gcgtacagtt tcccgcgcgc cgggtgatga
     7741 gacgccggaa aaggccgatc acggtggcat aagtggtaaa agttttattg atccggaagt
     7801 tttgcgtcgt cagcgtgcgg aagaaacccg cgtctacggc gaaaatgcat gtcaggcact
     7861 gttccagagc cgtccggaag caattgttcg cgcctggttt atccagagcg taacgccacg
     7921 ttttaaagaa gccttgcgct ggatggcggc aaaccgcaaa gcgtaccatg tggtggatga
     7981 agctgaattg acaaaagcgt caggcacgga acatcacggc ggcgtttgct tcctgatcaa
     8041 aaagcgtaac ggtacaaccg tgcagcagtg ggtaagccag gcaggcgcgc aggattgcgt
     8101 tctggcactg gaaaacgaat ctaacccgca taacctgggt ggcatgatgc gcagctgcgc
     8161 gcacttcggc gtgaaaggtg tcgtggtgca ggatgcggca ctgctggagt cgggggcggc
     8221 tatccgtacc gcagaaggcg gcgcagagca cgttcagccg attactggcg acaacattgt
     8281 taacgtgctg gatgatttcc gtcaggcagg ttacaccgta gtgaccactt ccagcgagca
     8341 gggtaaaccg ctgttcaaaa ctagtctgcc agcgaaaatg gtactggtgc tgggtcagga
     8401 atatgaaggg ttaccggatg ccgcacgcga tccgaacgat ctgcgcgtga agattgacgg
     8461 tacgggcaac gttgccgggc tgaatatctc tgtcgcaacc ggcgttttgc ttggtgaatg
     8521 gtggcgtcag aataaagcct gataaatcag ccgggtggca actctgccat ccggcatttc
     8581 cccgcaaatt tactcactct ctgccggtaa tactggcatc cagtcaatcg gcgtctcgcc
     8641 atgttgttcc agccactgat ttgccagcac aaaatggttg cagccaaaga acccacgatg
     8701 cgcagaaagc ggtgatggat gcggcgcttt cagtacgtga tggcgttgct tatcaataat
     8761 cgcccctttc ttttgcgcat gtgagcccca caacaaaaac accacgcctt cgcgatgctg
     8821 gttaatcagg ctgatgactt tatcggtgaa ggtctcccag ccgaggctgg cgtgggaatg
     8881 cgcctgacct gcgcgaaccg tcaacacagt attgagtagc agaacgccct gacgcgccca
     8941 gctttcaaga taaccatgat tagggcgggt gaaaccggga atagtatttt ccagctcttt
     9001 atacatattc agcagtgacg gcggtgtcgc aatgccgggg cgaacggaaa atgccagacc
     9061 atgcgcctgt cccggtccgt gataaggatc ctggccgaga atcaccactt taacgtcacc
     9121 caactctgta aagcggaacg cgttaaagac atccttttgt ggcggataga tagtgacgcc
     9181 ggactgccgc tcgctggcga cggtctgtag ggtattaaga aaatagggtt gttgcttctc
     9241 ttcagccagc acgtcatgcc aggttaattc gtttgccatc tcgctctccc gcgaatcttc
     9301 aatccgccta gcttaactgc ttctttctac agaacaaaat catgctcatt ttttagactt
     9361 aaacgagtgc aaaaaaatgt aaattggtaa aatttcacac ctgcataagt tgatgtaaaa
     9421 caataaaatc catttgcgac catacttgat gtgtggtttt tattgattta aatcaaagat
     9481 tcaagggtgt ttgaggagta tatatacact caagcaacaa tggttttacc aattggccgc
     9541 gacaggctga acaaatcaaa taattttgcc ggggaggcat cacatgatta caggtatcca
     9601 gattactaaa gccgctaacg acgatctgct gaactctttc tggctgctgg acagcgaaaa
     9661 aggcgaagcg cgttgcatcg ttgcgaaagc aggttttgca gaagacgaag tggttgcagt
     9721 aagcaaactg ggtgacattg agtaccgtga agttccagta gaagtgaaac cagaagttcg
     9781 cgttgaaggt ggtcaacacc tgaacgtaaa cgttctgcgt cgtgaaactc tggaagatgc
     9841 agttaagcat ccggaaaaat atccgcagct gaccatccgt gtatccggtt atgcagttcg
     9901 ctttaactct ctgactccgg aacagcagcg cgacgttatc gctcgtacct ttactgaaag
     9961 cctgtaaggc ttaagtagca agttactaag cggaagaggg cgacctcttc cgctttttca
    10021 ttaatagaaa atgcgtaccg cgcaatagac cagcagcagc gcgagcacga tatttaactg
    10081 gcgaccatac tggcgaaaca atcgctgaaa caggtgcccc gccagcgccc agcacacatt
    10141 gccaaacgtc ccaatcatcg ccagcaaaac gctgacgcca attatccagc ttagcgcctg
    10201 cgtttgcggc aggacaaacg tcgacagtgc tgtaatgcca tacaaaatga ttttgacgtt
    10261 cacaaactgc aaagcaaagc tggcccaaaa actgattggt tttgtctgaa gcccatcttc
    10321 ctttgttgga ctggtagcga ttttccacgc cagccagaca atatatgccg cccccgccca
    10381 actcaacaga tgcactgctg ccgggtcgat aacagccagt gaaaacgaaa ttcccgcgca
    10441 tagcaacatg acgattaaaa atcccagact catccctgcc agcacgcgag tactctgacg
    10501 aaatccgtgc gttgtggctg agctaagggc gagaatattg ttcggtcctg gcgtcatggc
    10561 ggtaatcagg gtgtaagtcc aaaaagcact taaaagggtc ggtgtcactt gttatctcct
    10621 ctgttttggt agacagtacc cgacacgtaa catttaaaaa aatgaatgtt tttacattct
    10681 aaattcgata ttttcgatag gtttggggtt atggatctgc gccgttttat tacgcttaaa
    10741 accgtggtgg aagagggttc ctttttgcga gcttcgcaaa aattgtgctg cacacaatcg
    10801 acggtgactt ttcatattca gcagcttgag caggagttct cagtccagtt atttgagaaa
    10861 attggttgac gaatgtgcct tacccgcgaa ggaaaaaagc tgctgccgca catctatgaa
    10921 ctcaccagag tgatggatac ccttcgtgag gctgccaaaa aagagtcgga tccggacggg
    10981 gaactacgcg ttgtttcagg cgaaacgttg ctttcttacc gaatgccgca ggtgttgcag
    11041 cgttttcggc aacgcgcacc aaaagtgcgt ttatcgttgc agtctctgaa ctgttacgtg
    11101 atccgtgatg cgctactgaa tgatgaggct gatgtcggcg tcttttaccg cgtagggaat
    11161 gatgatgctc tgaaccgacg agagttgggt gaacaatcac tggtgctggt ggcttcaccg
    11221 caaattaaag atgtcgattt tactgagccg ggaagacata acgcctgtag ctttattatc
    11281 aacgaaccgc aatgtgtctt ccggcagata tttgagagca cgctgcgaca gcggcggatc
    11341 acggtggaaa acaccattga gcttataagt atcgaaagca tcaagcgttg tgtcgcggcg
    11401 aatatcgggg tcagttactt gccccgcttt gcggtggaaa aagagcttga gagcggagaa
    11461 ttaattgaat tgccttttgg cgaacagtcg cagaccatta cggccatgtg cgcacaccat
    11521 gctggaaaag cggttagccc ggcgatgcac acttttattc agtgtgttga agagtgtttt
    11581 ttgccgggat aaaaataatg ccgggcttaa acccggcacc caacttgtcg acgaagtgcg
    11641 ttttgttcat gccggatgcg gcgtgaa
//
LOCUS       DAYPHN010000147        11543 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000147 DAYPHN010000000
VERSION     DAYPHN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.147"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..461)
                     /locus_tag="ACG0BW_003787"
     CDS             complement(<1..461)
                     /locus_tag="ACG0BW_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage resistance protein"
                     /protein_id="HFK7260696.1"
                     /translation="MNIIDTLQNDLERAVALQNILIARATGAQDEANNDYLLLRTYFL
                     QNPPLHQLLPEFVRTNRSLKEFWGFIKKTEKYAPRRDLIYKAFQPMLDYLEGKNKAPA
                     DEDISDVLRKFNEDGVHAVWIKALERRHTDPDGAITSARTLLETVCKHILD"
     gene            623..1411
                     /locus_tag="ACG0BW_003788"
                     /pseudo
     CDS             623..1411
                     /locus_tag="ACG0BW_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein E"
     gene            1453..1851
                     /locus_tag="ACG0BW_003789"
     CDS             1453..1851
                     /locus_tag="ACG0BW_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFK7260697.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELKEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1863..2216
                     /locus_tag="ACG0BW_003790"
     CDS             1863..2216
                     /locus_tag="ACG0BW_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFK7260698.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            2228..2806
                     /locus_tag="ACG0BW_003791"
     CDS             2228..2806
                     /locus_tag="ACG0BW_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260699.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2803..3198
                     /locus_tag="ACG0BW_003792"
     CDS             2803..3198
                     /locus_tag="ACG0BW_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFK7260700.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            3206..3946
                     /locus_tag="ACG0BW_003793"
     CDS             3206..3946
                     /locus_tag="ACG0BW_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260701.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            3962..4384
                     /locus_tag="ACG0BW_003794"
     CDS             3962..4384
                     /locus_tag="ACG0BW_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFK7260702.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            4366..4800
                     /locus_tag="ACG0BW_003795"
     CDS             4366..4800
                     /locus_tag="ACG0BW_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFK7260703.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4793..7354
                     /locus_tag="ACG0BW_003796"
     CDS             4793..7354
                     /locus_tag="ACG0BW_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFK7260704.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISIGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAYKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSSVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            7351..7680
                     /locus_tag="ACG0BW_003797"
     CDS             7351..7680
                     /locus_tag="ACG0BW_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7260705.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            7680..8378
                     /locus_tag="ACG0BW_003798"
     CDS             7680..8378
                     /locus_tag="ACG0BW_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFK7260706.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            8384..9127
                     /locus_tag="ACG0BW_003799"
     CDS             8384..9127
                     /locus_tag="ACG0BW_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFK7260707.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            9124..9696
                     /locus_tag="ACG0BW_003800"
     CDS             9124..9696
                     /locus_tag="ACG0BW_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFK7260708.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRIQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            9757..>11543
                     /locus_tag="ACG0BW_003801"
     CDS             9757..>11543
                     /locus_tag="ACG0BW_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFK7260709.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNDGWVTEKDITIKGKTT
                     SQYLASVVVGSLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEK"
BASE COUNT         2605 a         2875 c         3659 g         2404 t
ORIGIN      
        1 tcatcaagaa tgtgtttgca aactgtttca agaagtgttc ttgcggaggt tatcgcacca
       61 tccggatctg tatggcgccg ttcaagtgct tttatccaaa cggcgtggac tccatcctcg
      121 ttaaactttc ttaatacatc agatatgtct tcatccgctg gagctttatt tttcccttct
      181 agatagtcca gcatcggctg aaatgcctta taaatcaaat ctctccgggg ggcatacttt
      241 tcagtctttt ttataaatcc ccaaaattct tttagtgagc gattagttct cacaaactct
      301 ggtaatagct gatgtaaagg aggattttgt aagaaatatg ttcttagaag caaataatca
      361 ttattagctt catcctgggc tcctgtagct cttgcaatta gtatgttttg aagcgctact
      421 gcgcgctcta ggtcattttg aagggtatct ataatattca taacaaatct ttattcactt
      481 catttgtggg ttgataattg aatggtttgt acattgattt ctgtgtattg cagtgtgtat
      541 tgcaacatag ccattcaggt agagattatg ctctattttt cctcttatat cattcacata
      601 cttacaccat tgactcatgt agccgaagca tgaagtgaat ccgcagatga ccctgcgtcg
      661 cctgccggat gaagaaccgc agaatctggc ggacccggct taccgccgcc gtcgcatcat
      721 catgcagaac atgcgtgacg aagagctggc cattgctcag gtcgaagaga tgcaggcagt
      781 ttctgccgtg cttaagggca aatacaccat gaccggtgaa gccttcgatc cggttgaggt
      841 ggatatgggc cgcagtgagg agaataacat cacgcagtcc ggcggcacgg agtggagcaa
      901 gcgtgacaag tccacgtatg acccgaccga cgatatcgaa gcctacgcgc tgaacgccag
      961 cggtgtggtg aatatcatcg tgttcgatcc gaaaggctgg gcgctgttcc gttccttcaa
     1021 agccgtcaag gagaagctgg atacccgtcg tggctctaat tccgagctgg agacagcggt
     1081 gaaagacctg ggcaaagcgg tgtcctataa ggggatgtat ggcgatgtgg ccatcgtcgt
     1141 gtattccgga cagtacgtgg aaaacggcgt caaaaagaac ttcctgccgg acaacacgat
     1201 ggtgctgggg aacactcagg cacgcggtct gcgcacctat ggctgcattc aggatgcgga
     1261 cgcacagcgc gaaggcatta acgcctctgc ccgttacccg aaaaactggg tgaccaccgg
     1321 cgatccggcg cgtgagttca ccatgattca gtcagcaccg ctgatgctgc tggctgaccc
     1381 tgatgagttc gtgtccgtac aactggcgta atcatggccc ttcggggcca ttgtttctct
     1441 gtggaggagt ccatgacgaa agatgaactg attgcccgtc tccgctcgct gggtgaacaa
     1501 ctgaaccgtg atgtcagcct gacggggacg aaagaagaac tggcgctccg tgtggcagag
     1561 ctgaaagagg agcttgatga cacggatgaa actgccggtc aggacacccc tctcagccgg
     1621 gaaaatgtgc tgaccggaca tgaaaatgag gtgggatcag cgcagccgga taccgtgatt
     1681 ctggatacgt ctgaactggt cacggtcgtg gcactggtga agctgcatac tgatgcactt
     1741 cacgccacgc gggatgaacc tgtggcattt gtgctgccgg gaacggcgtt tcgtgtctct
     1801 gccggtgtgg cagccgaaat gacagagcgc ggcctggcca gaatgcaata acgggaggcg
     1861 ctgtggctga tttcgataac ctgttcgatg ctgccattgc ccgcgccgat gaaacgatac
     1921 gcgggtacat gggaacgtca gccaccatta catccggtga gcagtcaggt gcggtgatac
     1981 gtggtgtttt tgatgaccct gaaaatatca gctatgccgg acagggcgtg cgcgttgaag
     2041 gctccagccc gtccctgttt gtccggactg atgaggtgcg gcagctgcgg cgtggagaca
     2101 cgctgaccat cggtgaggaa aatttctggg tagatcgggt ttcgccggat gatggcggaa
     2161 gttgtcatct ctggcttgga cggggcgtac cgcctgccgt taaccgtcgc cgctgaaagg
     2221 gggatgtatg gccataaaag gtcttgagca ggccgttgaa aacctcagcc gtatcagcaa
     2281 aacggcggtg cctggtgccg ccgcaatggc cattaaccgc gttgcttcat ccgcgatatc
     2341 gcagtcggcg tcacaggttg cccgtgagac aaaggtacgc cggaaactgg taaaggaaag
     2401 ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc agaatcaaag ttaaccgggg
     2461 ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc ctttcgcgcc gcaggcgtcg
     2521 taaaaagggg cagcgttcat ccctgaaagg tggcggcagc gtgcttgtgg tgggtaaccg
     2581 tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc cggtggcatg tcatgcagcg
     2641 tgtggccggg aaaaaccgtt accccattga tgtggtgaaa atcccgatgg cggtgccgct
     2701 gaccacggcg tttaaacaaa atattgagcg gatacggcgt gaacgtcttc cgaaagagct
     2761 gggctatgcg ctgcagcatc aactgaggat ggtaataaag cgatgaaaca tactgaactc
     2821 cgtgcagccg tactggatgc actggagaag catgacaccg gggcgacgtt ttttgatggt
     2881 cgccccgctg tttttgatga ggcggatttt ccggcagttg ccgtttatct caccggcgct
     2941 gaatacacgg gcgaagagct ggacagcgat acctggcagg cggagctgca tatcgaagtt
     3001 ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt ggatggagtc ccggatttat
     3061 ccggtgatga gcgatatccc ggcactgtca gatttgatca ccagtatggt ggccagcggc
     3121 tatgactacc ggcgcgacga tgatgcgggc ttgtggagtt cagccgatct gacttatgtc
     3181 attacctatg aaatgtgagg acgctatgcc tgtaccaaat cctacaatgc cggtgaaagg
     3241 tgccgggacc accctgtggg tttataaggg gagcggtgac ccttatgcga atccgctttc
     3301 agacgttgac tggtcgcgtc tggcaaaagt taaagacctg acgcccggcg aactgaccgc
     3361 tgagtcctat gacgacagct atctcgatga tgaagatgca gactggactg cgaccgggca
     3421 ggggcagaaa tctgccggag ataccagctt cacgctggcg tggatgcccg gagagcaggg
     3481 gcagcaggcg ctgctggcgt ggtttaatga aggcgatacc cgtgcctata aaatccgctt
     3541 cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc agtatcggta aggcggtgac
     3601 ggcgaaggaa gtgatcaccc gcacggtgaa agtcaccaat gtgggacgtc cgtcgatggc
     3661 agaagatcgc agcacggtaa cagcggcaac cggcatgacc gtgacgcctg ccagcacctc
     3721 ggtggtgaaa gggcagagca ccacgctgac cgtggccttc cagccggagg gcgtaaccga
     3781 caagagcttt cgtgcggtgt ctgcggataa aacaaaagcc accgtgtcgg tcagtggtat
     3841 gaccatcacc gtgaacggcg ttgctgcagg caaggtcaac attccggttg tatccggtaa
     3901 tggtgagttt gctgcggttg cagaaattac cgtcaccgcc agttaatccg gagagtcagc
     3961 gatgttcctg aaaaccgaat catttgaaca taacggtgtg accgtcacgc tttctgaact
     4021 gtcagccctg cagcgcattg agcatctcgc cctgatgaaa cggcaggcag aacaggcgga
     4081 gtcagacagc aaccggaagt ttactgtgga agacgccatc agaaccggcg cgtttctggt
     4141 ggcgatgtcc ctgtggcata accatccgca gaagacgcag atgccgtcca tgaatgaagc
     4201 cgttaaacag attgagcagg aagtgcttac cacctggccc acggaggcaa tttctcatgc
     4261 tgaaaacgtg gtgtaccggc tgtctggtat gtatgagttt gtggtgaata atgcccctga
     4321 acagacagag gacgccgggc ccgcagagcc tgtttctgcg ggaaagtgtt cgacggtgag
     4381 ctgagttttg ccctgaaact ggcgcgtgag atggggcgac ccgactggcg tgccatgctt
     4441 gccgggatgt catccacgga gtatgccgac tggcaccgct tttacagtac ccattatttt
     4501 catgatgttc tgctggatat gcacttttcc gggctgacgt acaccgtgct cagcctgttt
     4561 ttcagcgatc cggatatgca tccgctggat ttcagtctgc tgaaccggcg cgaggctgac
     4621 gaagagcctg aagatgatgt gctgatgcag aaagcggcag ggcttgccgg aggtgtccgc
     4681 tttggcccgg acgggaatga agttatcccc gcttccccgg atgtggcgga catgacggag
     4741 gatgacgtaa tgctgatgac agtatcagaa gggatcgcag gaggagtccg gtatggctga
     4801 accggtaggc gatctggtcg ttgatttgag tctggatgcg gccagatttg acgagcagat
     4861 ggccagagtc aggcgtcatt tttccggtac ggaaagtgat gcgaaaaaaa cagcggcagt
     4921 cgttgaacag tcgctgagcc gacaggcgct ggctgcacag aaagcgggga tttccatcgg
     4981 gcagtataaa gccgccatgc gtatgctgcc tgcacagttc accgacgtgg ccacgcagct
     5041 tgcaggcggg caaagtccgt ggctgatcct gctgcaacag ggggggcagg tgaaggactc
     5101 cttcggcggg atgatcccca tgttcagggg gcttgccggt gcgatcaccc tgccgatggt
     5161 gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg tatgcctggt atcagggcaa
     5221 ctcaaccctg tccgatttca acaaaacgct ggtcctttcc ggcaatcagg cgggactgac
     5281 ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg gcagggctga cgtttaacca
     5341 gaccagcgag tcactcagcg cactggttaa ggcgggggta agcggtgagg ctcagattgc
     5401 gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc ggcgtggagg tggacaaggt
     5461 cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg gggctgacgg cgatggctcg
     5521 ccagttccat aacgtgacgg cggagcagat tgcgtatgtt gctcagttgc agcgttccgg
     5581 cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca acgaaagggt ttgatgacca
     5641 gacccgccgc ctgaaagaga acatgggcac gctggagacc tgggcagaca ggactgcgcg
     5701 ggcattcaaa tccatgtggg atgcggtgct ggatattggt cgtcctgata ccgcgcagga
     5761 gatgctgatt aaggcagagg ctgcgtataa gaaagcagac gacatctgga atctgcgcaa
     5821 ggatgattat tttgttaacg atgaagcgcg ggcgcgttac tgggatgatc gtgaaaaggc
     5881 ccgtcttgcg cttgaagccg cccgaaagaa ggctgagcag cagactcaac aggacaaaaa
     5941 tgcgcagcag cagagcgata ccgaagcgtc acggctgaaa tataccgaag aggcgcagaa
     6001 ggcttacgaa cggctgcaga cgccgctgga gaaatatacc gcccgtcagg aagaactgaa
     6061 caaggcactg aaagacggga aaatcctgca ggcggattac aacacgctga tggcggcggc
     6121 gaaaaaggat tatgaagcga cgctgaaaaa gccgaaacag tccagcgtga aggtgtctgc
     6181 gggcgatcgt caggaagaca gtgctcatgc tgccctgctg acgcttcagg cagaactccg
     6241 gacgctggag aagcatgccg gagcaaatga gaaaatcagc cagcagcgcc gggatttgtg
     6301 gaaggcggag agtcagttcg cggtactgga ggaggcggcg caacgtcgcc agctgtctgc
     6361 acaggagaaa tccctgctgg cgcataaaga tgagacgctg gagtacaaac gccagctggc
     6421 tgcacttggc gacaaggtta cgtatcagga gcgcctgaac gcgctggcgc agcaggcgga
     6481 taaattcgca cagcagcaac gggcaaaacg ggccgccatt gatgcgaaaa gccgggggct
     6541 gactgaccgg caggcagaac gggaagccac ggaacagcgc ctgaaggaac agtatggcga
     6601 taatccgctg gcgctgaata acgtcatgtc agagcagaaa aagacctggg cggctgaaga
     6661 ccagcttcgc gggaactgga tggcaggcct gaagtccggc tggagtgagt gggaagagag
     6721 cgccacggac agtatgtcgc aggtaaaaag tgcagccacg cagacctttg atggtattgc
     6781 acagaatatg gcggcgatgc tgaccggcag tgagcagaac tggcgcagct tcacccgttc
     6841 cgtgctgtcc atgatgacag aaattctgct taagcaggca atggtgggga ttgtcgggag
     6901 tatcggcagc gccattggcg gggctgttgg tggcggcgca tccgcgtcag gcggtacagc
     6961 cattcaggcc gctgcggcga aattccattt tgcaaccgga ggatttacgg gaaccggcgg
     7021 caaatatgag ccagcgggga ttgttcaccg tggtgagttt gtcttcacga aggaggcaac
     7081 cagccggatt ggcgtgggga atctttaccg gctgatgcgc ggctatgcca ccggcggtta
     7141 tgtcggtaca ccgggcagca tggcagacag ccggtcgcag gcgtccggga cgtttgagca
     7201 gaataaccat gtggtgatta acaacgacgg cacgaacggg cagataggtc cggctgctct
     7261 gaaggcggtg tatgacatgg cccgcaaggg tgcccgtgat gaaattcaga cacagatgcg
     7321 tgatggtggc ctgttctccg gaggtggacg atgaagacct tccgctggaa agtgaaaccc
     7381 ggtatggatg tggcttcggt cccttctgta agaaaggtgc gctttggtga tggctattct
     7441 cagcgagcgc ctgccgggct gaatgccaac ctgaaaacgt acagcgtgac gctttctgtc
     7501 ccccgtgagg aggccacggt actggagtcg tttctggaag agcacggggg ctggaaatcc
     7561 tttctgtgga cgccgcctta tgagtggcgg cagataaagg tgacctgcgc aaaatggtcg
     7621 tcgcgggtca gtatgctgcg tgttgagttc agcgcagagt ttgaacaggt ggtgaactga
     7681 tgcaggatat ccggcaggaa acactgaatg aatgcacccg tgcggagcag tcggccagcg
     7741 tggtgctctg ggaaatcgac ctgacagagg tcggtggaga acgttatttt ttctgtaatg
     7801 agcagaacga aaaaggtgag ccggtcacct ggcaggggcg acagtatcag ccgtatccca
     7861 ttcaggggag cggttttgaa ctgaatggca aaggcaccag tacgcgcccc acgctgacgg
     7921 tttctaacct gtacggtatg gtcaccggga tggcggaaga tatgcagagt ctggtcggcg
     7981 gaacggtggt ccggcgtaag gtttacgccc gttttctgga tgcggtgaac ttcgtcaacg
     8041 gaaacagtta cgccgatccg gagcaggagg tgatcagccg ctggcgcatt gagcagtgca
     8101 gcgaactgag cgcggtgagt gcctcctttg tactgtccac gccgacggaa acggatggcg
     8161 ctgtttttcc gggacgtatc atgctggcca acacctgcac ctggacctat cgcggtgacg
     8221 agtgcggtta tagcggtccg gctgtcgcgg atgaatatga ccagccaacg tccgatatca
     8281 cgaaggataa atgcagcaaa tgcctgagcg gttgtaagtt ccgcaataac gtcggcaact
     8341 ttggcggctt cctttccatt aacaaacttt cgcagtaaat cccatgacac agacagaatc
     8401 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgcggct tcgtggtaag
     8461 cacgccggag ggggaaagat atttcccctg cgtgaatatc tccggtgagc cggaggcgta
     8521 tttccgtatg tcgccggaag actggctgca ggcagaaatg cagggtgaga ttgtggcgct
     8581 ggtccacagc caccccggtg gtctgccctg gctgagtgag gccgaccggc ggctgcaggt
     8641 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcataagt tccgctgtgt
     8701 gccgcatctc accgggcggc gctttgagca cggtgtgacg gactgttaca cactgttccg
     8761 ggatgcttat catctggcgg ggattgagat gccggacttt catcgtgagg atgactggtg
     8821 gcgtaacggc cagaatctct atctggataa tctggaggcg acggggctgt atcaggtgcc
     8881 gttgtcagcg gcacagccgg gcgatgtgct gctgtgctgt tttggttcat cagtgccgaa
     8941 tcacgccgca atttactgcg gcgacggcga gctgctgcac catattcctg aacaactgag
     9001 caaacgagag aggtacaccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     9061 ggcatggcgc gcatctgcct ttacggggat ttacaacgat ttggtcgccg catcgacctt
     9121 cgtgtgaaaa cgggggctga agccatccgg gcactggcca cacagctccc ggcgtttcgt
     9181 cagaaactga gcgacggctg gtatcaggta cggattgccg ggcgggacgt cagcacgtcc
     9241 gggttaacgg cgcagttaca tgagactctg cctgatggcg ctgtaattca tattgttccc
     9301 agagtcgccg gggccaagtc aggtggcgta ttccagattg tcctgggggc tgccgccatt
     9361 gccggatcat tctttaccgc cggagccacc cttgcagcat ggggggcagc cattggggcc
     9421 ggtggtatga ccggcatcct gttttctctc ggtgccagta tggtgctcgg tggtgtggcg
     9481 cagatgctgg caccgaaagc cagaactccc cgtatacaga caacggataa cggtaagcag
     9541 aacacctatt tctcctcact ggataacatg gttgcccagg gcaatgttct gcctgttctg
     9601 tacggggaaa tgcgcgtggg gtcacgcgtg gtttctcagg agatcagcac ggcagacgaa
     9661 ggggacggtg gtcaggttgt ggtgattggt cgctgatgca aaatgtttta tgtgaaaccg
     9721 cctgcgggcg gttttgtcat ttatggagcg tgaggaatgg gtaaaggaag cagtaagggg
     9781 cataccccgc gcgaagcgaa ggacaacctg aagtccacgc agttgctgag tgtgatcgat
     9841 gccatcagcg aagggccggt tgaaggtccg gtggatggat taaaaagcgt gctgctgaac
     9901 agtacgccgg tgctggacac tgaggggaat accaacatct ccggtgtcac ggtggtgttc
     9961 cgtgccggtg agcaggagca gacaccgccg gaggggtttg aatcctccgg ctccgagacg
    10021 gtgctgggta cggaagtgaa atatgacacg ccgatcaccc gcaccattac gtctgcaaac
    10081 atcgaccgtc tgcgctttac cttcggtgta caggcactgg tggaaaccac ctcaaagggt
    10141 gacaggaatc cgtcggaagt ccgtctgctg gttcagatac aacgtaacga tggctgggtg
    10201 acggaaaaag acatcaccat taagggcaaa accacctcgc agtatctggc ctcggtggtg
    10261 gtgggtagcc tgccgccgcg cccgtttaat atccggatgc gcaggatgac gccggacagc
    10321 accacagacc agctgcagaa caaaacgctc tggtcgtcat acaccgaaat catcgatgtg
    10381 aaacagtgct acccgaacac ggcactggtc ggcgtgcagg tggactcgga acagttcggc
    10441 agccagcagg tgagccgtaa ttatcatctt cgcgggcgca ttctgcaggt gccgtcgaac
    10501 tataacccgc agacgcggca atacagcggt atctgggacg gaacgtttaa gccggcatac
    10561 agcaacaacc cggcctggtg tctgtgggat atgctgaccc acccgcgcta cggcatgggg
    10621 aaacgtcttg gtgcggcgga tgtggataaa tgggcgctgt atgtcatcgg ccagtactgc
    10681 gaccagtcag tgccggacgg ttttggcggc acggagccgc gcatcacctg taatgcctac
    10741 ctgaccacgc agcgtaaggc gtgggatgtg ctcagtgatt tctgctcggc gatgcgctgt
    10801 atgccggtat ggaacgggca gacgctgacg ttcgtgcagg accgaccatc agataaggtg
    10861 tggacctata accgcagtaa tgtggtgatg ccggatgatg gcgcgccgtt ccgctacagc
    10921 ttcagcgccc tgaaggaccg ccataatgcc gttgaggtga actggattga cccgaacaac
    10981 ggctgggaga cggcgacaga gcttgttgaa gatacgcagg ccattgcccg ttacggtcgt
    11041 aatgtcacga agatggatgc ctttggctgt accagccggg ggcaggcaca ccgcgccggg
    11101 ctgtggctga ttaaaacgga actgctggag acgcagaccg tggatttcag cgtgggtgct
    11161 gaagggcttc gccatgtacc gggcgatgtc attgaaatct gcgatgatga ctatgccggt
    11221 atcagcaccg gtggtcgcgt gctggcggtg aacagccaga cccggacgct gacgctcgac
    11281 cgtgaaatca cgctgccatc ctccggtacc acgctgataa gcctggttga cggaagtggt
    11341 aatccggtca gcgtggaggt tcagtccgtc accgacggac ttaaggtgaa agtgaaccgg
    11401 gttcctgacg gcgttgcaga atacagtgtg tgggggctga agttgccgac gttgcgtcag
    11461 cgcctgttcc gctgtgtgag tatccgtgag aacgatgacg gcacgtatgc catcactgcc
    11521 gtgcagcatg taccggaaaa aga
//
LOCUS       DAYPHN010000148        11348 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000148 DAYPHN010000000
VERSION     DAYPHN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.148"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            134..1276
                     /gene="yghO"
                     /locus_tag="ACG0BW_003802"
     CDS             134..1276
                     /gene="yghO"
                     /locus_tag="ACG0BW_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFK7260710.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1283..2047)
                     /gene="glcC"
                     /locus_tag="ACG0BW_003803"
                     /pseudo
     CDS             complement(1283..2047)
                     /gene="glcC"
                     /locus_tag="ACG0BW_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            2298..3797
                     /gene="glcD"
                     /locus_tag="ACG0BW_003804"
     CDS             2298..3797
                     /gene="glcD"
                     /locus_tag="ACG0BW_003804"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFK7260711.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIKVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3797..4849
                     /gene="glcE"
                     /locus_tag="ACG0BW_003805"
     CDS             3797..4849
                     /gene="glcE"
                     /locus_tag="ACG0BW_003805"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFK7260712.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSARLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4860..6083
                     /gene="glcF"
                     /locus_tag="ACG0BW_003806"
     CDS             4860..6083
                     /gene="glcF"
                     /locus_tag="ACG0BW_003806"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFK7260713.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6088..6492
                     /locus_tag="ACG0BW_003807"
     CDS             6088..6492
                     /locus_tag="ACG0BW_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFK7260714.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6514..8685
                     /gene="glcB"
                     /locus_tag="ACG0BW_003808"
     CDS             6514..8685
                     /gene="glcB"
                     /locus_tag="ACG0BW_003808"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFK7260715.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHDILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9041..10723
                     /gene="glcA"
                     /locus_tag="ACG0BW_003809"
     CDS             9041..10723
                     /gene="glcA"
                     /locus_tag="ACG0BW_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFK7260716.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALILIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(11167..>11348)
                     /locus_tag="ACG0BW_003810"
     CDS             complement(11167..>11348)
                     /locus_tag="ACG0BW_003810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260717.1"
                     /translation="SGKDPVPESTGGVSEEDPEPPPSQPANRVALKIAAKSDFLYLNF
                     LFINNASDKTKLKDS"
BASE COUNT         2669 a         2817 c         3135 g         2727 t
ORIGIN      
        1 tctctaacct tatcataatc attaataaat actatttatt ctttgtttgt attcattggg
       61 ttgattatta aggtgtcgtt cgttgctgtt gcaacctgtt tttcaccgtt aattatttgt
      121 tatccacaga gatatggaat gcgatctgct gatgattaaa attgaaaaag taattaataa
      181 aaatgacctt aaggcattta tcgcttttcc gtcatcactt tatcccgacg atccaaactg
      241 gataccccct ttattcatag aacgcaacga gcatttgtct gcgaaaaatc cggggacgga
      301 ccatatcatc tggcaggcgt gggtggcaaa aaaagcgggg cagatagttg ggcgtattac
      361 cgcgcaaata gataccttgc accgtgagcg ttacggtgaa gataccggtc atttcggcat
      421 gattgacgcc attgatgatc cgcaggtttt tgccgcgttg tttggcgcag cggaagcgtg
      481 gttgaagtca caaggtgcaa gtaagatcag cgggcctttc agcctgaata tcaatcagga
      541 aagcggatta ctgattgaag gttttgacac accaccctgt gcgatgatgc cacacggcaa
      601 accgtggtat gccgcgcata ttgaacaact gggttatcac aaaggcattg atttactggc
      661 gtggtggatg cagcgaaccg atctcacttt ctctccggcg ctaaaaaaac tgatggatca
      721 ggtgcgcaaa aaggtgacca ttcgctgcat caatcgtcag cggtttgccg aagagatgca
      781 gatcctgcgt gagattttca actctggctg gcagcacaac tggggatttg tgccgtttac
      841 cgaacatgaa ttcgcgacca tgggcgatca acttaaatat ctggtgccgg acgatatgat
      901 ctacatcgct gagattgatt ctgcaccctg cgcgtttatt gtcggcttgc cgaacatcaa
      961 cgaggcgatt gccgatctga acggatcgct ctttcccttc ggctgggcaa aattgctgtg
     1021 gcgtttgaaa gtcagcggtg tgcgaactgc gcgagtaccg ctgatgggcg tgcgcgacga
     1081 gtatcagttc agccgcatcg gcccggtgat tgcgctgtta ttgattgaag ccttacgcga
     1141 tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca tggatcctcg aaaccaatac
     1201 aggcatgaat aacatgctgg aacgcattgg ggcggagccg tacaagcgtt accgattgta
     1261 tgaaaagcag atttaatacc agctaactca ggttcatctc cagcggcacc gccgagcgaa
     1321 tcaaatggtg gccttccagc tcgatatcgt ggagattctt tttcacggtc cgcacatgat
     1381 cgcgtgctgc gcgctgggcg acgtgcggca gccgctgcaa caccgcgttg tagatcctcg
     1441 catgctggcg atcgatctgc tttttttgct gtggtcaatg gtagagatta tttacgctgg
     1501 caaacactga attaaacatc agatcggtca atgattgcag cgtaaacacc agcacctgat
     1561 tgtgagaagc ctgacaaatg gcgagatgga aagcgtgatc caactgcgca tgttcgatca
     1621 gcgaaatctc tttgttgttc tcactggcgg cgagcatttt ttcataacag cgggttatca
     1681 caacaaaatc agcctgcgtt cccagcgttg ccgccagcct tgccgattcg ccctccagta
     1741 atgcgcgaac gtcgagcaga tcgtacagcg ttcgcggctg cgtactgaac agatggatca
     1801 gcgggctggt gtcctgcacc cgattaagcc gtgcgacacg agaatcgcga ccctgcgccg
     1861 tttcaataat cccgcgcccg cgcagcacgg tcagcccttc acgcagtgcg gagcgtgaga
     1921 agccgagctt ttcacacagt cgacgttccg agggaagtgg ctgaccgacc ttcagtacgc
     1981 cgtcgataat taaccgttcg atactctctg caaccacttc gcaaataggg cgacgttcat
     2041 ctttcattcc cggacctcgt gcacaggtag gaccaatttt tctgttctta actcgcaaaa
     2101 cacgcacatc acgtaagtga ttatgttaca tcaatttaac attgagttaa ccaaggcaag
     2161 gtcacagagc tggaaaaaaa atggtctgac cggtagctaa agagatagac gaaaacgaaa
     2221 agcccgctta ataactgttc acagaagcag cgcgcaaaaa tcagctgcca cacaacacaa
     2281 caaagcgaag cctactcatg agcatcttgt acgaagagcg tcttgatggc gctttacccg
     2341 atgtcgaccg cacatcggta ctgatggcac tgcgtgagca tgtccctgga cttgagatcc
     2401 tgcataccga tgaggagatc attccttacg agtgtgacgg gttgagcgcg tatcgcacgc
     2461 gtccattact ggttgttctg cctaagcaaa tggaacaggt gacagcgatt cttgctgtct
     2521 gccatcgcct gcgtgtaccg gtggtgaccc gtggtgcagg caccgggctt tctggtggcg
     2581 cgctgccgct ggaaaaaggt gtgttgttgg tgatggcgcg ctttaaagag atcctcgaca
     2641 ttaaccccgt tggtcgccgc gcgcgcgtgc agccaggcgt gcgtaacctg gcgatctccc
     2701 aggccgttgc accgcataat ctctactacg caccggaccc ttcctcacaa atcgcctgtt
     2761 ccattggcgg caacgtggca gaaaatgccg gcggcgtcca ctgcctgaaa tatggtctga
     2821 ccgtacataa cctgctgaaa attaaagtgc aaacgctgga cggcgaggcg ctgacgctgg
     2881 gatcggacgc gctggattca cctggttttg acctgctggc gctgttcacc ggatcggaag
     2941 gtatgctcgg cgtgaccacc gaagtgacgg taaaactgct gccgaagccg cccgtggcgc
     3001 gggttctgtt agccagcttt gactcggtag aaaaagccgg acttgcagtt ggtgacatca
     3061 tcgccaatgg cattatcccc ggcgggctgg agatgatgga taacctgtcg atccgcgcgg
     3121 cggaagattt tattcatgcc ggttatcccg tcgacgccga agcaattttg ttatgcgagc
     3181 tggacggcgt ggagtctgac gtacaggaag actgcgagcg ggttaacgac atcttgttga
     3241 aagcgggcgc gactgacgtc cgtctggcac aggacgaagc agagcgcgta cgtttctggg
     3301 ccggtcgcaa aaatgcgttc ccggcggtag gacgtatctc cccggattac tactgcatgg
     3361 atggcaccat cccgcgtcgc gccctgcctg gcgtactgga aggcattgcc cgtttatcgc
     3421 agcaatatga tttacgtgtt gccaacgtct ttcatgccgg agatggcaac atgcacccgt
     3481 taatcctttt cgatgccaac gaacccggtg aatttgcccg cgcggaagag ctgggtggga
     3541 agatcctcga actctgcgtt gaagttggcg gcagcatcag tggggaacat ggcatcgggc
     3601 gagaaaaaat caatcaaatg tgcgcccagt tcaacagcga tgaaatcacg accttccatg
     3661 cggtcaaggc ggcgtttgac cccgatggtt tgctgaaccc tgggaaaaac attcccacgc
     3721 tacaccgctg tgctgaattt ggtgccatgc atgtgcatca cggtcattta cctttccctg
     3781 aactggagcg tttctgatgc tacgcgagtg tgattacagt caggcgctgc tggagcaggt
     3841 gaatcaggca attagcgata aaacgccgct ggtgattcag ggcagcaata gcaaagcctt
     3901 tttaggtcgc cctgtcaccg ggcaaacgct ggatgtgcgt tgtcatcgcg gcattgttaa
     3961 ttacgacccg accgagctgg tgataaccgc gcgtgccgga acgccgctgg tggcgattga
     4021 agcggcgctg gagagcgccg ggcaaatgct tccctgcgag ccgccgcatt atggtgaaga
     4081 agccacctgg ggcgggatgg tcgcctgcgg gctggcgggg ccgcgtcgcc cgtggagcgg
     4141 ttcggtccgc gattttgtcc tcggcacgcg catcattacc ggcgctggaa aacatctgcg
     4201 ttttggtggc gaagtgatga aaaacgttgc cggatacgat ctctcacggt taatggccgg
     4261 aagctacggt tgtcttggcg tgctcactga aatctcgatg aaagtgttac cgcgaccgcg
     4321 cgcctccctg agcctgcgtc gggaaatcag cctgcaagaa gccatgaacg aaatcgccca
     4381 gtggcaactc cagccgctac ctattagtgg cttatgttac ttcgacaatg cgttgtggat
     4441 ccgccttgag ggcggcgaag gatcggtaaa agcggcgcgt gaactgctgg ggggcgaaga
     4501 ggttgcgggt cagttctggc agcaattgcg tgaacaacaa ctgccgttct tctcgttacc
     4561 aggtacctta tggcgcattt cattacccag cgatgcgccg atgatggatt tacccggcga
     4621 gcaactgatc gactggggcg gagcgttacg ctggctgaaa tcgacagccg aggacaatca
     4681 aatccatcgc atcgcccgca acgctggcgg tcatgcgacc cgctttagtg ccggagatgg
     4741 tggctttgcc ccgctatcgg ctcgtttatt ccgctatcac cagcagctta aacagcagct
     4801 cgacccttgc ggcgtgttta accccggtcg catgtacgcg gaactttgag gagcaggcta
     4861 tgcaaaccca attaactgaa gagatgcggc agaacgcgcg cgcgctggaa gccgacagca
     4921 tcctgcgcgc ctgtgttcac tgcggatttt gtaccgcaac ctgcccaacc tatcagcttc
     4981 tgggcgatga actggacggg ccgcgcgggc gcatctatct gattaaacag gtgctggaag
     5041 gcaacgaagt cacgcttaaa acacaggagc atctcgatcg ctgcctcact tgccgcaatt
     5101 gtgaaaccac ctgtccttct ggtgtgcgct atcacaattt gctggatatc gggcgtgata
     5161 ttgtcgagca gaaagtgaaa cgcccactgc cggagcgaat gctgcgcgaa ggattgcgcc
     5221 aggtagtgcc gcgtccggcg gtcttccgtg cgctgacgca ggtagggctg gtgctgcgac
     5281 cgtttttacc ggaacaggtc agagcaaaac tgcctgctga aacggtgaaa gctaaaccgc
     5341 gtccgccgct gcgccataag cgtcgggttt taatgctgga aggctgcgcc cagcctacgc
     5401 tttcgcccaa caccaacgcg gcaactgcgc gagtgctgga tcgtctgggg atcagcgtca
     5461 tgtcagctaa cgaagcaggc tgttgtggcg cggtggacta tcatcttaat gcgcaggaga
     5521 aagggctggc acgggcgcgc aataatattg atgcctggtg gcccgcgatt gaagcaggtg
     5581 ccgaggcaat tttgcaaacc gccagcggtt gcggcgcgtt tgtcaaagag tacgggcaga
     5641 tgctgaaaaa cgatgcgtta tatgccgata aagcgcgtca agtcagtgaa ctggcggtcg
     5701 atttagtcga gcttctgcgc gaggaaccgc tggaaaaact ggcagttcgc ggcgataaaa
     5761 aactggcctt ccactgcccg tgtaccctac aacatgcgca aaagctgaac ggcgaagtgg
     5821 aaaaagtgtt gcttcgtctt ggatttacct taacggacgt tcccgacagc catctgtgct
     5881 gcggttcagc gggaacatat gcgttaacgc atcccgatct ggcacgccag ctgcgggata
     5941 acaaaatgaa tgcgctggaa agcggcaaac cggaaatgat cgtcaccgcc aacattggtt
     6001 gccagacgca tctggcgagc gccggtcgta cttctgtgcg tcactggatt gaaattgtag
     6061 aacaagccct tgaaaaggaa taaaaaaatg aaaactaaag tcattcttag ccagcaaatg
     6121 gcgagtgcaa ttattgccgc aggtcaggaa gaggcgcaga aaaataactg gtctgtttcc
     6181 attgctgttg ccgatgacgg cggtcatctg ctggcgttaa gtcgcatgga cgattgcgcg
     6241 ccgattgcgg cttacatctc ccaggagaaa gcgcgtaccg ccgcgctggg gcgtcgtgaa
     6301 actaagggct atgaagagat ggtgaacaac ggacgtaccg cgttcgtgac tgcgccgtta
     6361 ctgacgtcgc tggaaggtgg cgtaccggtt gttgttgatg ggcaaattat tggtgccgtg
     6421 ggcgtttctg gtttaaccgg agcacaggat gcgcaggtcg cgaaagcggc agcagcggtg
     6481 ttggcgaaat aagcgaaaac gaggagataa acaatgagtc aaaccataac ccagggccgt
     6541 ttacgcattg acgccaattt taaacgtttt gtggatgaag aagttttacc gggaacaggg
     6601 ctggacgctg cggcgttctg gcgcaatttt gatgaaatcg ttcacgatct ggcaccagaa
     6661 aatcgtcagt tgctggcaga acgcgatcgc attcaggcgg cgcttgatga gtggcatcgc
     6721 agcaatccgg ggccggtaaa agataaagcg gcctataaat ctttcctgcg tgaactgggc
     6781 tacctggtgc cgcaaccgga gcgcgtgacg gtggaaacca cgggcattga cagcgaaatc
     6841 accagtcagg cggggccaca gctggtggtt ccggcaatga acgcccgcta cgcgctgaac
     6901 gcggcgaacg ctcgctgggg ctcactgtac gatgcgttat acggcagcga catcatcccg
     6961 caggaagggg cgatggtcag cggctacgat ccgcaacgcg gtgagcaggt tatcgcctgg
     7021 gttcggcgtt tcctcgatga atctctaccg ctggaaaacg gcagctatca ggatgtggtg
     7081 gcgtttaagg tggtcgataa acaattacgc atccagttga aaaatggtaa agaaaccacg
     7141 ttacgtaccc cggcgcagtt tgtcggttac cgtggcgatg ccgctgcgcc gacctgcatt
     7201 ttgctgaaaa ataacggcct gcatattgaa ctgcaaattg atgccaacgg gcggattggc
     7261 aaagacgatc cggcgcacat caacgatgtt atcgtcgaag cggccatcag taccattctc
     7321 gactgcgaag attcggtcgc ggcggttgat gcggaagata aaatcctgct gtaccgcaat
     7381 ctgctgggcc tgatgcaggg gactctgcaa gagaaaatgg agaaaaacgg tcgacaaatc
     7441 gtgcgtaaac tgaatgacga tcgtcattac accgccgccg atggctctga aatttctctg
     7501 cacggacgct cgctgctgtt tatccgcaac gtgggtcatt tgatgaccat tcctgtgatt
     7561 tgggacagcg aaggcaatga aatcccggaa ggcattcttg atggcgtcat gactggcgcg
     7621 attgccctct atgatttaaa agtgcagaaa aactcgcgca ctggcagcgt ctatattgtg
     7681 aaaccgaaaa tgcacggtcc gcaggaagtg gcgttcgcca acaaactgtt tacccgcatt
     7741 gagacaatgc tcggtatggc accgaatacc ctgaaaatgg gcattatgga tgaagaacgt
     7801 cggacctcgc tgaacttgcg tagctgtatc gctcaggcgc gcaaccgcgt ggcgttcatc
     7861 aataccggtt tcctcgaccg taccggcgat gaaatgcatt cggtgatgga agctggcccg
     7921 atgctgcgta aaaaccagat gaaatcgacg ccttggatca aagcctacga acgtaataac
     7981 gtgctttccg gcctgttctg tgggctgcgc ggtaaagcgc aaatcggtaa aggcatgtgg
     8041 gcaatgccgg acctgatggc agacatgtac agccagaagg gcgaccaact gcgtgccggg
     8101 gcaaacacag cctgggttcc gtcgccaact gccgctacgc tccatgcgat gcactaccac
     8161 caaaccaacg tacagagcgt gcaagccaac attgcccaga ccgagttcaa tgctgaattt
     8221 gaaccgctgc tggacgatct gctgactatt ccggtggctg aaaacgctaa ctggtcggtg
     8281 gaagagatcc aacaagagct ggataacaac gtgcagggga ttctggggta cgtggtgcgc
     8341 tgggtggagc aggggattgg ttgttcaaaa gtgccggata ttcacaatgt ggcgctgatg
     8401 gaagaccgcg caacgctgcg tatctccagc cagcatatcg ccaactggtt acgtcacgat
     8461 attctgacca aagaacaggt gcaggcgtcg ctggagaata tggcgaaagt ggttgatcag
     8521 caaaacgctg acgatccggc ttatcgtccg atggcgggga atttcgctaa ctcgtgtgct
     8581 tttaaagctg ccagcgattt aatcttcctc ggcgtgaaac agccaaacgg ctataccgaa
     8641 ccgttattac acgcctggcg tttacgcgaa aaagaaagtc attaatagcg tcaaaaaaag
     8701 ccccggcaag ttttattgtc ggggctatga tagtaactgc ggtagtacat ttatcccgga
     8761 aataaccgga gcgattatca gggtttaaca atattaaata cccgtagcgc attactagcg
     8821 ggcctataac aacaatatgg acatcttaac gatactctgc gcctgcgtgt gcagaacaga
     8881 aacccttacc cgatatactt gttatgccag aggtagcaat tgcgttagcg gcgtactgtt
     8941 gctgtaaagt ataacgccta tattttacag ttcagtcctc ttttgcttat tacaacctta
     9001 agtatatcaa gcatataaag ataataagag actgaacaat atggttacct ggacccaaat
     9061 gtatatgccg atgggaggac tggggctatc cgctctggtc gccctgatcc tgataatatt
     9121 cttcttcgtt gcactcgcgg tattacgtct gaaaggacat gtcgctggag caataaccct
     9181 tatattatct atcctgattg caatattcgc ctttaaaatg ccgattgata tggcttttgc
     9241 tgctgcgggc tatggcttta tttatggatt atggcctatt gcgtggatta ttgtcgcggc
     9301 ggtgttcctc tataaattaa ccgttgccag cggtcagttc gatattatcc gcagctcggt
     9361 tatctccatc accgacgatc agcgtttgca ggtgttactg attggtttct cctttggtgc
     9421 gttgctggaa ggagcggctg gctttggtgc gccggtggcg attaccggtg cgctgctggt
     9481 gggcctgggc ttcaaaccgt tatacgcggc ggggctgtgt ctgattgcca acactgcgcc
     9541 agtggcgttt ggtgcgttgg gcgtgccgat tctggtcgcc ggtcaggtaa cgggaatcga
     9601 tccgttccac attggtgcta tggcaggacg ccagttgccg ttcctgtcgg tactggtgcc
     9661 gttctggctg gtagcaatga tggacggctg gaaaggggtg aaagagacgt ggccagcggc
     9721 gctggttgct gggggaagct tcgctgtcac tcagttcttt acctctaact atattggtcc
     9781 ggaactgccg gatattactt cggcgctggt gagtatcgtc tcactcgctt tattccttaa
     9841 agtctggcgg ccgaaaaata ccgaaacggc aatcagcatg ggacaatccg caggtgcgat
     9901 ggtggtgaat aaaccatctt ctggtggtcc cgtgccttca gaatatcgtc tggggcaaat
     9961 cattcgtgcg tggtcaccgt ttttaatctt aacggtgctg gtcaccatct ggaccatgaa
    10021 gccgtttaaa gcgttatttg ctccgggcgg cgcgttttat tcactggtga ttaatttcca
    10081 gatccctcat ttgcatcaac aagtgttgaa agcggcaccc attgtcgccc aaccaacgcc
    10141 aatggatgcg gtgtttaaat tcgaccctct ctcggctggc ggcaccgcta tttttattgc
    10201 ggcgattatc tctatcttca tcctcggcgt gggcatcaag aaagggattg gcgtctttgc
    10261 cgaaacgcta attagtctga agtggccgat attgtcgatt ggcatggtgc tggcgttcgc
    10321 cttcgtcacc aactattccg gtatgtccac cacgctggcg ctggtactgg caggtacagg
    10381 cgtgatgttc ccgtttttct caccgtttct cggctggctg ggcgtattcc ttaccggctc
    10441 ggacacctcc tctaacgccc tgtttggttc actgcaatcg accacggcgc agcaaatcaa
    10501 cgtctctgac actctgctgg tggcagcaaa taccagcggc ggcgtaactg gcaagatgat
    10561 ctccccgcaa tctatcgccg tggcctgcgc cgcgacgggc atggtgggcc gagaatctga
    10621 actgttccgc tacaccgtga agcacagtct gatttttgcc agcgttatcg gcattatcac
    10681 gctgttgcag gcgtatgtgt ttactgggat gttagtctcg taaatcaata atgccggatg
    10741 atcgttaata tcatccggct tcatttacat tactctgtct tattaatgga aaattatttc
    10801 ccgctttatt atccatgtgg caatgatgtt tcgcgttcag ataacatgct aaataattaa
    10861 gagcgtgaga acatctatta tttatcacgc aaaacaagtc agtattaatt atgttcgctt
    10921 gatgagcaaa caccgttcta tctgttgctt ttgcaatctt aatcgtcaag actaccaaca
    10981 aatttatata gttacgttgt gaaaggttgt taattttttg tttattgaaa tttcgtaaaa
    11041 ataaccagcc agacatcgaa taggggttat tcttataaat ttcacctgtt tttctatcaa
    11101 tgatgccgtt ttcttaagaa tggaggaagt tattaaagaa gataaagcat aaaactattt
    11161 ccccggttac gaatctttta actttgtttt gtcacttgcg ttattaatga ataagaaatt
    11221 taaatataag aaatcgcttt tagcggctat tttgagtgca accctgttag ccggttgtga
    11281 tggcggtggc tccggatctt cctccgatac gccgcctgta gattctggaa cagggtcttt
    11341 gccggaag
//
LOCUS       DAYPHN010000149        11281 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000149 DAYPHN010000000
VERSION     DAYPHN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.149"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..820
                     /locus_tag="ACG0BW_003811"
     CDS             <1..820
                     /locus_tag="ACG0BW_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFK7260718.1"
                     /translation="YMEYLVTCPLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGG
                     DLQALASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAAL
                     PFSRPLVYWDGQVLKSTTEIKNDDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKEG
                     IWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKN
                     MGSNSVTIINNNTRKSIPPGKVFTLRGGALNINIPGRSALLLGKTGEPPNYFYL"
     gene            1156..2133
                     /gene="glaH"
                     /locus_tag="ACG0BW_003812"
     CDS             1156..2133
                     /gene="glaH"
                     /locus_tag="ACG0BW_003812"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFK7260719.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2153..3421
                     /gene="lhgO"
                     /locus_tag="ACG0BW_003813"
     CDS             2153..3421
                     /gene="lhgO"
                     /locus_tag="ACG0BW_003813"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFK7260720.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3444..4892
                     /gene="gabD"
                     /locus_tag="ACG0BW_003814"
     CDS             3444..4892
                     /gene="gabD"
                     /locus_tag="ACG0BW_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFK7260721.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFEDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALERGARVVCGGKADDRGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4906..6186
                     /gene="gabT"
                     /locus_tag="ACG0BW_003815"
     CDS             4906..6186
                     /gene="gabT"
                     /locus_tag="ACG0BW_003815"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFK7260722.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6497..7897
                     /gene="gabP"
                     /locus_tag="ACG0BW_003816"
     CDS             6497..7897
                     /gene="gabP"
                     /locus_tag="ACG0BW_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFK7260723.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLTAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7918..8580
                     /gene="csiR"
                     /locus_tag="ACG0BW_003817"
     CDS             7918..8580
                     /gene="csiR"
                     /locus_tag="ACG0BW_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFK7260724.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8581..9030)
                     /gene="kbp"
                     /locus_tag="ACG0BW_003818"
     CDS             complement(8581..9030)
                     /gene="kbp"
                     /locus_tag="ACG0BW_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFK7260725.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9114..9272)
                     /gene="yqaE"
                     /locus_tag="ACG0BW_003819"
     CDS             complement(9114..9272)
                     /gene="yqaE"
                     /locus_tag="ACG0BW_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFK7260726.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9455..9754
                     /gene="ygaV"
                     /locus_tag="ACG0BW_003820"
     CDS             9455..9754
                     /gene="ygaV"
                     /locus_tag="ACG0BW_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFK7260727.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9764..10288
                     /gene="ygaP"
                     /locus_tag="ACG0BW_003821"
     CDS             9764..10288
                     /gene="ygaP"
                     /locus_tag="ACG0BW_003821"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFK7260728.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(10335..10739)
                     /gene="stpA"
                     /locus_tag="ACG0BW_003822"
     CDS             complement(10335..10739)
                     /gene="stpA"
                     /locus_tag="ACG0BW_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFK7260729.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2734 a         2805 c         2964 g         2778 t
ORIGIN      
        1 ttacatggaa tacctggtaa cttgtccgtt agatgaacaa caaatgtcat cgctgcttta
       61 tgaaagagat gatttaagcg ccattgattt ttcattgctc atgaagacga taaaagcctt
      121 ttcattcggt ggagatctcc aagcccttgc atcaaaaccg ggctccacaa tctcaagcat
      181 cccatcagaa agacggatat tgattaacat caaccacgat ttccctaaca atggtaatct
      241 tttcaatgac tttttattta accatcagca agatgaacaa ttagcaatgg catatatggc
      301 cgctctcccg ttcagcaggc ctttagttta ctgggatggt caggtattaa aatcaacgac
      361 tgagattaaa aatgatgatg ggtccactcg tgtcggcggt gaagcgtggc ttaataaagg
      421 ttgctctacc tatcagcagc tctacaatga attccacgca ttatatatag ataaagaagg
      481 aatatggagc gcatttgagg gtgtatttgc aactaaaaat gttctggcct ttagtcgtgg
      541 ggattctgtg aacattaatc actctcctca tgatggacta gttataataa ataaaggaaa
      601 cgaagaagtt gaaggtacct ggcctaacaa attgcaacca ggaatatata aaaacatggg
      661 gagtaatagc gttaccatta ttaataataa tacacgcaaa agtattcccc ctggtaaagt
      721 atttacgctt agaggtggag ctctaaatat caatattcct gggcgcagcg ctcttctttt
      781 agggaaaaca ggagaaccgc cgaactattt ctatttgtaa tttaatgtta tatctgcccc
      841 gctaaaacgg ggcagataat atgtttagtt tactaacggt cattttgcag tgaagccatt
      901 tactgttttt tatcgaccag ataatctgtt ctctgatggt agctccccct aacctgttgc
      961 tttagttatt catttcctgt ctcactttgc cttaataccc cacattaaat gttactaatt
     1021 tgttgtcttt gatcacaata agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat
     1081 gtagatattt ttagattatg gctacgaaat gagcatcgcc atgtcaccct acatctcata
     1141 agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt cgattcaggc
     1201 caggactata gcggattcac tctcatcccg tcggcgcaat ccccgcgtct gctggaactc
     1261 accttcactg aacagacgac caaacagttt ctcgagcagg ttgccgaatg gcccgtacag
     1321 gcgctggagt acaaatcgtt tctgcgtttt cgggtaggaa aaattcttga cgatctgtgt
     1381 gcgaatcagc tgcaaccgtt gctgttaaag accctgctaa accgcgctga aggtgcgttg
     1441 ttgatcaatg cggtgggtgt cgatgatgtg aagcaagcgg atgagatggt gaagctggcg
     1501 acggcggtgg cgcatctgat tggtcgctcc aatttcgatg cgatgagcgg tcagtattac
     1561 gcgcgttttg tagtgaaaaa tgtcgataac tcagacagct atctgcgcca gccgcaccgc
     1621 gtgatggaac tgcacaacga cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg
     1681 atgaaaatcg atgagcagaa catgcagggt ggaaattcgt tgctgctgca tctcgatgac
     1741 tgggaacatc tggaccacta tttccgccat ccactggcgc gtcgcccgat gcgctttgcc
     1801 gcgccgccga gcaaaaacgt cagcaaagat gttttccatc cagtgttcga cgttgatcaa
     1861 cagggtcgcc cggtgatgcg ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa
     1921 ggcgtgtggt tgagcgagct atccgatgcc attgaaacca gcaaaggcat tctttctgta
     1981 cctgttcccg ttggcaaatt cctgttgata aacaacctgt tctggctgca cggtcgcgac
     2041 cgctttactc cgcacccgga tctgcgccgt gaactgatgc gccagcgtgg ctatttcgct
     2101 tacgccactc accactacca gacgcatcag taagcgcaaa ggaattgagc ggatgtatga
     2161 ttttgtgatt attggcggcg gcatcatcgg catgtcgacc gccatgcaac tgattgatgt
     2221 ttatccggat gcccgcattg cgttgctgga aaaagagtcc ggcccggcct gtcaccagac
     2281 gggccacaac agtggcgtga tccatgccgg ggtctattac acgcccggta gcctgaaggc
     2341 acagttttgc ctggcgggaa accgcgccac caaagccttt tgcgatcaaa acggcattcg
     2401 ctatgacaac tgcggaaaga tgctggtcgc cacctctgaa ctcgaaatgg aacggatgcg
     2461 cgcgttgtgg gaacgcacgg cggctaacgg gatcgagcgc gagtggttaa acgctgagga
     2521 actgcgagag cgcgaaccga atatcaccgg gctcggcggt atttttgtgc cgtccagcgg
     2581 cattgtcagc taccgcgaag tgacagcggc aatggcaaaa atcttccagg ccagaggcgg
     2641 tgagattatc tataacgccg aagtcagcgg ccttagtgag cataaaaacg gtgtggtgat
     2701 acgtacccga caaggcggcg aatatgaagc atcgacgctg attagctgtt ccgggctgat
     2761 ggctgaccgg ctggtgaaaa tgctcggcct cgagccgggc tttattatct gcccgttccg
     2821 cggcgagtat ttccgccttg cgccggagca taaccagatt gttaaccacc tgatttaccc
     2881 cattcccgac cccgcgatgc catttttggg cgtgcatctc acccgcatga tcgacggcag
     2941 cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga
     3001 cttctcattt agcgacacgc tggaaatttt gggctcgtcg gggattcgcc gggtgctgca
     3061 aaaccatcta cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct
     3121 gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc cctggcccgc
     3181 cggtgtgcgg gcgcaggcag tatcgccgga cggcaagctg attgacgatt ttctgtttgt
     3241 caccacccca cgcacgatcc acacctgcaa tgcgccctcc ccggcagcga catcagcaat
     3301 tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc
     3361 cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat ttaatcaata
     3421 acctttgaaa acaggacgta acaatgaaac ttaacgacag taacttattc cgccagcagg
     3481 cgttgattaa cggggaatgg ctggacgcca acaatggcga ggtcatcgac gtcaccaatc
     3541 cggcgaacgg cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg
     3601 ctatcgacgc cgccaaccgc gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg
     3661 ccaacattct gcgcaactgg ttcaatttga tgatggagca tcaggacgat ttagcgcgcc
     3721 tgatgaccct cgaacagggc aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg
     3781 cctcttttat tgagtggttt gccgaagaag gcaaacgtat ttatggcgac accattcccg
     3841 gtcatcaggc cgacaaacgc ctgattgtta tcaaacagcc gattggcgtt accgccgcca
     3901 tcacgccgtg gaacttcccg gcggcgatga ttacccgtaa agccggtccg gcgctggcgg
     3961 caggctgcac gatggtgctg aaacctgcca gtcagacgcc gttctctgcg ctggcgctgg
     4021 cggaactggc gattcgcgca ggcgttccgg ctggcgtatt taacgtggtc accggttcgg
     4081 cgggcgcggt cggtaacgaa ctgaccagca atccgctggt gcgcaagctg tcgtttaccg
     4141 gctcgaccga aattggtcgc cagttaatgg aacagtgcgc gaaagatatt aaaaaagtgt
     4201 cgctggagct gggcggcaac gcgccattta tcgtctttga ggatgccgac ctcgacaaag
     4261 ccgtggaagg cgcgctggcc tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca
     4321 accgtttata cgtgcaggac ggcgtgtatg accgctttgc cgaaaaatta cagcaggcgg
     4381 tgagcaaact gcacatcggc gacgggctgg agaatggcgt caccatcggg ccgctgatcg
     4441 atgaaaaagc ggtagcaaaa gtggaagagc atattgccga tgcgctggag agaggtgcgc
     4501 gtgtggtttg cggcggtaaa gcggatgacc gcggcggcaa cttcttccag ccgaccattc
     4561 tggtggacgt tccggccaac gccaaagtgt cgaaagaaga gacgttcggc ccccttgccc
     4621 cgctattccg ctttaaagat gaagccgatg tgattgcgca ggccaatgac accgagtttg
     4681 gccttgccgc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg ggcgaggcgc
     4741 tggagtacgg catcgtcggc attaatactg gcattatttc caatgaagtg gccccgttcg
     4801 gcggcattaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc gaagattact
     4861 tagaaatcaa atatatgtgc atcggtcttt aactggagaa cgcgaatgag cagcaataaa
     4921 gaattaatgc agcgccgcag tcaggcagtt cctcgtggcg ttgggcaaat tcaccccatt
     4981 ttcgctgatc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga gtatcttgat
     5041 ttcgcgggcg gcattgcagt gctcaatacc gggcacctgc atccgaaagt ggttgcggcg
     5101 gtggaagcgc agttgaaaaa gctgtcgcac acctgcttcc aggtgctggc ctacgagccg
     5161 tatctggagc tgtgcgaaat aatgaatcag aaggtgccgg gcgattttgc caaaaaaacg
     5221 ctgctggtaa cgaccggttc tgaagcggtg gagaacgcgg tgaaaatcgc ccgcgccgcc
     5281 accaaacgca gcgggaccat cgcctttagc ggcgcgtatc acgggcgcac gcattacacg
     5341 ctggcgctga ccggcaaggt gaatccttac tctgcgggca tgggcctgat gccagggcat
     5401 gtttatcgcg cactttatcc gtgtccactg cacggcataa gcgaggatga cgctatcgcc
     5461 agcatccacc ggatcttcaa aaatgacgcc gcgccggaag atatcgccgc catcgtgatt
     5521 gagccggttc agggtgaagg cggtttctac gccgcgacgc cagcctttat gcagcgttta
     5581 cgcgcgttgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca gagcggcgcg
     5641 gggcgtaccg gcacgctgtt tgcgatggag cagatgggcg ttgcgccgga tcttaccacc
     5701 tttgcgaaat cgatcgcagg cggcttcccg ctggcgggcg tcaccgggcg cgcggaagtg
     5761 atggatgccg tcgctccagg cggtctgggc ggcacctatg cgggtaaccc gattgcctgc
     5821 gtggctgcgc tggaagtgtt gaaggtgttt gagcaggaaa atctgctgca aaaagccaac
     5881 gatctggggc agaaactgaa agacggattg ctggcgatag ccgaaaaaca tactgagatc
     5941 ggcgacgtac gcgggctggg agcgatgatt gccatcgagc tgtttgaaga tggcgatcac
     6001 agcaaaccag acgcaaaact caccgccgag atcgtggcgc gtgctcgcga taagggcttg
     6061 attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc gctcaccatt
     6121 gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttgc agaggcgaag
     6181 cagtagtcat gtccgttcgt aagagttaac cgttgctaag ggaatgataa cgatgcagta
     6241 aaaaataata acgatgaggc gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc
     6301 ccctctgggg agagggttag ggtgaggggt aaaaaacgcg actaacccct cccccggccc
     6361 tatccctgag ggagaaggag aaaaccatcc cgctttcgtt tttaagattt tgttgaccgt
     6421 ctcaattaca cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact
     6481 ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa
     6541 gtcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg
     6601 ttccagcgtc gccatcgccg aagcgggccc ggcggtatta ctggcctatc tgttcgccgg
     6661 attactggtg gttatgatta tgcggatgtt ggcggaaatg gcggttgcca cgcccgacac
     6721 gggttcgttt tccacctatg cagataaagc tattggtcgt tgggcgggct ataccatcgg
     6781 ctggttgtac tggtggtttt gggtactggt tatcccgctg gaagccaaca tcgccgccat
     6841 gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct
     6901 cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct
     6961 ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag
     7021 cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt
     7081 tatgcccaac ggcttcggcg cagtattaac cgcgatgttg atcaccatgt tctcgtttat
     7141 gggcgcagaa attgtcacca ttgccgccgc ggaatctgac acgccggaaa aacatattgt
     7201 ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt
     7261 cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc
     7321 ggttctggag ttgctcaata ttccccatgc gaaattaatc atggactgcg tgatattact
     7381 ttccgtaacc agttgcctga actcggcgct gtataccgct tccagaatgc tctactcctt
     7441 aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta
     7501 tgtggcggta ttactctcga ccggtgcggc atttttaacg gtggtggtga attattacgc
     7561 gcctgcgaaa gtgtttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta
     7621 tttagtcatc gccgtttcac aattgcggat gcgcaaaatt ctgcgagcag aaggaagcga
     7681 aattcgcctg cggatgtggc tttatccgtg gctcacctgg ctggtcatcg gctttattac
     7741 ctttgtgttg gtagtgatgc tattccgccc ggcgcaacag ttagaagtga tctccaccgg
     7801 cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt
     7861 attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg
     7921 accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt
     7981 caaccggatg aaaaattacg gatgagtttg cttacatcgc gttatgcact tggcgttggg
     8041 ccgttacggg aagctctttc gcaactggtg gcggaacggc tggtcacggt ggtgaatcaa
     8101 aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc
     8161 gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag
     8221 gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aggcctgtga cgccagtgag
     8281 aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacggcaat tgtggcgggc
     8341 tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat
     8401 cgatttatct ggctgcggcg aacagtgctt tcggtggaga tgctggagga taaacacgat
     8461 cagcaccaga ccctgacagc ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta
     8521 atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa
     8581 ctactcttca ggaatacgca acacttgccc cgggtaaatt ttatccgggc ttttgagcat
     8641 cggtttattc gcttcgaaga ttttattgta cagattggcg ttaccgtaga cctgtttgga
     8701 aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag cggctggtgt
     8761 cgccgttttc acctgatcat cgacactggc aataccggaa atattcccca ccgcaacaag
     8821 gattttctcc ttcgcctcct gactcaggcc atcacccgtg accgtcgctt tgccgtcggc
     8881 gatttgaatg ttcactttat cggcatcagg tataccggtt ttgttcagat gctcctgcac
     8941 cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc
     9001 ggcatctttc acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca
     9061 gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgaggaca ggtttaatcg
     9121 cgggtttgca cccagaaagc gtgaatcaaa ccggggatat atccaagcag tgtcagcaga
     9181 atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgag cggcggcaga
     9241 atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat
     9301 tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct gccagtttca
     9361 gcttacgctt ttactctcat ttacgctgct gatgctggca tatgttatat ttttagttta
     9421 gagtttactt aatttagaaa atgcttaaat aactatgact gaactcgcgc aattacaggc
     9481 cagtgccgaa caggcagcgg ccttattgaa agcaatgagc caccctaaac ggttgctgat
     9541 tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc gcattaccgg
     9601 actgagtgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag ggcttatcga
     9661 cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg taaatgccat
     9721 tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt tgacaaccat
     9781 ttcgccgcat gatgcacaag aattaatcgc acgcggcgca aagttaatcg atattcgtga
     9841 cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat tatccgtgct
     9901 ggaacagtca ggtcttccgg ctaaattacg tcgcgagcaa attatattcc actgccaggc
     9961 aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc ccgcagaaat
    10021 ctttttactc gaagatggga ttgatggctg gaaaaaagca ggattaccag tagcggtaaa
    10081 taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt
    10141 aatcggcgtt atactgggct ataccgtaaa tagcggtttc ttcttattaa gcggctttgt
    10201 tggtgccggg ttactgtttg caggaattag cggtttttgt gggatggcaa ggttgttaga
    10261 taagatgccg tggaaccaac gagcttgaga agcgacgccg gacgcgccat agcggcggca
    10321 tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc cagcgcctga
    10381 gcgattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc gttaacatca
    10441 gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcgcgtgg tgcagcagca
    10501 gagctattac ccaataactc ttccgggtta attccgtcag ctttcatcag ctccagccag
    10561 gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc ttcttcacgt
    10621 ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc aatggagaat
    10681 tcgcgagcca tcgcacggag agtgcgaatg ttatttaaac tttgtaacat tacggacata
    10741 ataaagaaaa ccttttaacg ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc
    10801 acttattata tttccacaaa atttaatcta tttacagtcc tgcacgagat tatttcagct
    10861 gttattttct gaaactctca gaataatttc aagattatat attagccatt ttgatgagta
    10921 atttcggtta ttggtgaaca gagcggcgct ctctgctcgc taattccgca agcctgacaa
    10981 aaccaaagtg aataaaaatg caatttaacc gaataaatga aaacacccct cactggtgct
    11041 ttctgctgtc aatgtacatt ttctggcata aaaaatgctg aaaatgaccc ggtcacgaca
    11101 tacaccgaat tctctacgct tatagcgtta cctcaccccc aaactttcat tagtgtaatt
    11161 aaaggcagga tcatatctaa ttgattcaat tgaaaataaa ccatatcggc atgtaaaaat
    11221 ccacaatgta caaaaaacgc gccgttacgg aatattttat ctacaaaaac agtttaaata
    11281 a
//
LOCUS       DAYPHN010000150        11224 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000150 DAYPHN010000000
VERSION     DAYPHN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.150"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            110..280
                     /locus_tag="ACG0BW_003823"
                     /pseudo
     CDS             110..280
                     /locus_tag="ACG0BW_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            270..776
                     /locus_tag="ACG0BW_003824"
     CDS             270..776
                     /locus_tag="ACG0BW_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFK7260730.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            959..1774
                     /locus_tag="ACG0BW_003825"
     CDS             959..1774
                     /locus_tag="ACG0BW_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFK7260731.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2121..4007
                     /locus_tag="ACG0BW_003826"
     CDS             2121..4007
                     /locus_tag="ACG0BW_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HFK7260732.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4048..4575
                     /locus_tag="ACG0BW_003827"
     CDS             4048..4575
                     /locus_tag="ACG0BW_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HFK7260733.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4679..6058
                     /locus_tag="ACG0BW_003828"
     CDS             4679..6058
                     /locus_tag="ACG0BW_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HFK7260734.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            6061..7344
                     /locus_tag="ACG0BW_003829"
     CDS             6061..7344
                     /locus_tag="ACG0BW_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7260735.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            7334..8464
                     /locus_tag="ACG0BW_003830"
     CDS             7334..8464
                     /locus_tag="ACG0BW_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFK7260736.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALFAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKLVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            8469..9164
                     /locus_tag="ACG0BW_003831"
     CDS             8469..9164
                     /locus_tag="ACG0BW_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFK7260737.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            9151..9636
                     /locus_tag="ACG0BW_003832"
     CDS             9151..9636
                     /locus_tag="ACG0BW_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFK7260738.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAAFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPSELEATFARLAA
                     R"
     gene            9661..10146
                     /locus_tag="ACG0BW_003833"
     CDS             9661..10146
                     /locus_tag="ACG0BW_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260739.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            10268..10901
                     /locus_tag="ACG0BW_003834"
                     /pseudo
     CDS             10268..10901
                     /locus_tag="ACG0BW_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
     gene            complement(10845..11069)
                     /locus_tag="ACG0BW_003835"
     CDS             complement(10845..11069)
                     /locus_tag="ACG0BW_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260740.1"
                     /translation="MGEISVKTGNNTRNGGKNSLNRLIRCVCGTKIGPDPRNFNQRVS
                     LGRHDLLIRTFPKGVFGFGQRTLLFFFAGR"
     gene            complement(11051..>11224)
                     /locus_tag="ACG0BW_003836"
     CDS             complement(11051..>11224)
                     /locus_tag="ACG0BW_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7260741.1"
                     /translation="SIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLF
                     RVDQMIRQWERSQ"
BASE COUNT         2476 a         2795 c         3366 g         2587 t
ORIGIN      
        1 catcgctaac tttgcaacag tgccgtgcag acagaaaccg taaacaccgg cattgacatg
       61 ccggggaagc ccttatagta caataattct ctatatccaa actgaccccg atgagaaaag
      121 catggagacg ttgagcacca atctgcaact cgcccggctg gttggcgttc aggggacgcc
      181 ggcaaccatc attggcgacg agatgatccc cggcgcggta tcgtgggaga cccttgaggc
      241 ggtggtgaaa gaaaaactgg cggtggccca tgctcagtaa actgcgccgc tggctgcgtg
      301 aaggcgcgat cttactggtt gtgctggctg gcgtgataat cctgctggat gtctggcgtt
      361 ctccgcagat gccggccatg tttgacagca cgccgctgca tacgctggac ggggaaaccg
      421 taacgctggc gtcgatcagt gaagaacgcc cggtgctgct ctacttctgg gcgagctggt
      481 gcggtatctg ccgtttcacc acgccggatg tcgcccgttt gcagtcggaa ggggagagcg
      541 tcatgaccat cgcgctgcgt tccggtaatg acggggaggt ttcccgctgg ttatcgcgta
      601 aaagggtgac gttccccgtc gtgaatgata gcggcggtga gatatcacga aactgggaaa
      661 tcagcgtcac gccaacgctg gtggtggtgt cgaaggggca ggtggtaact acgaccagcg
      721 gctggaccag ctactggggc atgaagttac gtctgtggcg ggcagccatg ttttgatagc
      781 gtgccgggga aacccggcat tttttaaccc attgtaagga tgtgtaaaac gagcggagag
      841 cgtgttttct gttatttttg ctgctttttt accgacgcca gttattattt tttaccgctg
      901 cctgacgttg caaacctgaa ttgataatgg catatctgtt ttaattgaat gaaagtctgt
      961 gatacgttca ggcgattttt tcgatttcca ttttgacagg gataacaatt caatgcgcaa
     1021 gggtaaaatt attgttgcat caatgatggt gctttgtgtc cttatttttg ttgcggcggc
     1081 ggcatggttt atgctgttcc agggagaaat gacggagccg acagaggagg aaaaacaggc
     1141 ggcaatttta cacgctgcgg gcctgatgaa atcagagacc caggataaaa aatcagtcga
     1201 aacgctctat catcgttata ttattcaacg ccatgttaat ttagattccg gcgaactggt
     1261 ggctggcagc agcgcggaca ccgcgcggca gaagtgtgaa aaacttgccc ctgaacgcga
     1321 cccagcgcag gttcgtcagc gctgtacggt ggcggatgtg ttctttgtga aagataaaaa
     1381 taatgaaatt cagcaggtta tcattccagt taccggtaag ggtgcgaaat ccatgatgca
     1441 tgcgtttctt gctctggggc tggacggtcg tacggtgaga aatctgtatt attatcagca
     1501 acgggaaacg ccgtttctgg gggctagggt ggaagacgcc aactggcgca aacagtggcc
     1561 agggaaacga ttattagata acagcggaca tcctgcattg aaaatagtac aggataaacc
     1621 tgaacatgcc gatgaatata ccgttgacgg tatttccggt gcgacgttga catcaaccgg
     1681 cgttgagaaa agtattaact actggatggg gccgcaggga tatggtcaat tcttacagcg
     1741 tcttgccagc gatcgaaata accttaatct ttgaatattg ctgtacgcaa taacgcgttt
     1801 tatttctgcc tgccgataat gaataatatc actgtcggca gggggattta ttcctctgtt
     1861 accgcgcctg ccttgccgtt aattccatga gaaaaacagg gagataacgt cccttttatc
     1921 aacgcataat gtgtgtgttc atcacgattt gatgaaataa gtttacataa cttgtcgtct
     1981 gctgacattt ttatgatcca ggtcatgtat aacgcgacaa aaagatatga taataattat
     2041 catttgtatt ctttggcggt gtgcacgtgc gtaacttccg taactctttg ttggttactc
     2101 tctttggctt gtttataagc atgagtgtct gggcgtcgac gaattatgcg cctttgattg
     2161 aagatatcga gcaacgtctt gataaaacag cggaacttta tcagcaacag catgccgacg
     2221 aggcgcgccg cacggtacaa atggcctatt ttgaggtgtt cgagaacctg gaagggccga
     2281 tccgcatcaa catttccgcg cgcaaaagtt acgagatgga gagcgcattc ggcgagatcc
     2341 gtcgaatgat tggcgagaaa aaaccgctcg ctgacgtcca ggcgcgcatt gactggctga
     2401 aagccgcact gcgcgaagtg gaaccggtgc tggatggcgg acatcgtctg gtggccgaag
     2461 agcagcacaa cgcgctgtcc agggacgaca ttgccgttca ctggcaggag agtttcagga
     2521 cgatcgacga tctgctggcg caggcggtca ctgaatatca ggccgggaat tacagcgttg
     2581 ccagtcagca cgttcaacag gcgcactatc agggctttaa aaactctgag atggagatgt
     2641 ccctcagaca aaaccgttct gcaaaagatg ccgcctccat taaccaacaa ttctcatccc
     2701 tgattgcgct gaccgcgcaa cctgaccatc tcaacgatgt ctcctatcag gtcactacgc
     2761 tgctacagga tatcgaggat atcttacccg gcttgccaac cacccgtgac gatcagcagg
     2821 ttgccgcagc gcaaagcgca gacaacattc cggcggcgga cgagggaaat aaccgcaccg
     2881 actgggctga ggtgacgacg ggaattaacc agagcattca ggacgcgata gcgcgctatc
     2941 agggcggcga tgcgaagaat gcgattctgg atatccagga tacctacttc gaccgcttcg
     3001 aagccagcgg catggaaaac aaaatcggct ctcgcgattc ggcatttaaa accacgctgg
     3061 aggcgtattt tacccgcctg gtcagtctga tgaaggccgg tcagccggtt gagcgcctga
     3121 atgcagaggc cagcgcgctg acgcaggatc tgcaaaaagc cgtgacgatg ctgggcgaag
     3181 gcgaagaaac ccagtggagt ctgctgctct atagtctgat gatcatcgtg cgcgaggggc
     3241 tggaagcgct gctgattgtt gcggcaatcg tggcctacat ggtgaaaaac aaccatcagg
     3301 ataagctgcc gcttattcgt cagtcggtaa ttgtggcgct gatcgccagc gtcatcaccg
     3361 ccgctatctt ccagatgctg tttactaact ccggcgccag ccgtgaactg ctggaaggca
     3421 taacgatgct gattgccgtc gtgatgctgt tctttatgag ttactggctg ctgtccaaag
     3481 tggaagcgcg ccactggaaa gcctggctgg aaggcaaact gtctcattcc ctgtcgcgag
     3541 gctccctggt cggactatgg ctgaccagct tcctggcggt gtaccgcgaa ggggcggaaa
     3601 cggtgctgtt ctattacgct ctgattggcg atgcaaacac ggtatccggc catatggcga
     3661 ttggcgccgg ttttgtgatt ggctgcgtgg tgctgctgct cgcctggctg gtgatgcgtt
     3721 actcagtggt gcgcctgccg ctgaaaccct tctttatgtt taccggcagt ttcatgtatc
     3781 tgatggcctt cgtctttgcg ggcaaaggcg ttctggaact ggtggaaggc aaactgttcc
     3841 agcctacgtt gattaacggc ttccctgaaa tcagctggct ggggatttat ccctacgtgg
     3901 aaacgttgtt accgcaggtc gtattgctgc tcgcggcgct ggtggcgctg tgggtgatgc
     3961 ggcgcaaaag cgcggttccc ggggagacga taaaaaataa cccataatcg ttgtatagcc
     4021 gttgttttag acgagaggat acgtttgatg accatgaaga aaaccctgat tgccagcgca
     4081 gtgatggcca gcattttcat tgcgccagcc gcgtttgctt ttaaagagta cccggcaggc
     4141 gagcctgtca ccatgaatga aatggagctg gcggccgttt acctgcaacc gattgatatg
     4201 gaaccgcgcg gcatgggctt accggcagca aaagccgatg ttcaccttga agcggatatc
     4261 cacgctgtag aaggcaataa aaacggcttt ggcgcagggg aatggatccc gtacctgacc
     4321 atcagctaca ccctggtcaa caacgacacg ggcgaaaaac aggaaggcac cttcatgccg
     4381 atggttgcca gcgatggccc gcactatggc gcgaacatca aaatgatggg cgtgggcaac
     4441 tataaagtga cctaccacat tgaaccgccg tcaaaagccg ggatgcatcg ccatactgac
     4501 agcgaaacag gcgtaggccg ctggtggaaa ccgtttgatg tgagctacga gtttaaatat
     4561 gtcggtctga actaaaaccg actaaccaac gcgccaggag gattccccct tctggcgcgt
     4621 tggtcagacc gatcatatgc ccgtcgctta attcaacccg ccgtacagag tcgaagttat
     4681 gagttacttt ttcgtcacga cgctacaggt ctttttctgc attgcgcttc tttccggtgt
     4741 tctctggagt cgaaacgatc ctccctcttt acgtccgctg acctggacgc tgctgaccgg
     4801 gctgattgcc ggcgtgctgg cgggactgtt tattcacggc agccaaccgg tacaactgct
     4861 gctggttggg gcggaggtca tggtatcgct gctgtttgtc ctcagtttct ggtgggcttc
     4921 aacgcgtatc cgctatctgt ggcaggggat tctgattttc ggcgctgccc gccattgggc
     4981 gctggaccct aatctgggcg ggctgaccag tacgcatgtg ctgaatactg acctgctgtt
     5041 aaacctgacg gccgtcgttc tcgcttttgc gatcctctgt ctggcgggcg tactctgcgc
     5101 gatgctgctg cggcgtattc gcggcctgta ctggccgtta acgctgattc tgctggtgat
     5161 gatctggctg ccgctgagcg gcaacctgct gttgttgctg atgaaattac aggttgttcc
     5221 gctgggaaaa tcactgctga gcttcgtggc gaaagtcaca aataacacgg cgctgtataa
     5281 ctgggcggga gccgcgctgc tgctggccct ggcgctgtgc tggcttccgg cgctgttgcg
     5341 ggcttttcga cagacgcggg aaaccgaaga accgattgct caccgtctgg cgctggcgca
     5401 gcgtcgcaac gctttgcggc tgtggttggt cacaatcggc tgcgcggtgg tcgttatcgc
     5461 aggccaactg tggtgggata aagtggcctc gcagccgccg caactctctg aagccgtgcc
     5521 cgtaacgctg gggagcgatg gcatggtgcg cctgccggtt gaacagcttc gtgacggtaa
     5581 actgcatcgt ttcgtctggg tggcggacga tggcaaagcg gtgcgcttct tcgtgattaa
     5641 ccgctatccc gacaagctgc gttttggcgt ggtgttcgac gcctgcctgc tgtgcggcga
     5701 tcagggttat gtcatggagg gcaatcaggt tatctgcgtc gcctgcggag tgcatatttt
     5761 tattccgtct atcggcaagg ccggtggctg taacccggtg ccgattgaaa actggcacaa
     5821 tgatgaaaaa gagctggtga tccccggtaa agaactggcg acaggggtga actacttctc
     5881 gacggtcatg actattaaag tgaccgatcc ggtcgatggt tcaacgttga ccaatacctc
     5941 agccgattac aaatacagct atggcggcaa gacctggttc ttctcgtcag aggctaacta
     6001 tgaacgcttc cgtgaaacgc cggagcagtt tgttcctgcc gacatgaggg aggaataatc
     6061 atgctgtggc ggatgttacg acaatcctgg gggcgcaatt tacgccgcaa agtgctggcg
     6121 ataatcaccg tttttctggc ctccagcctg atctcggcgc tgctggcggt gtccattgat
     6181 atcggcgaca aaatgtccag agagctgaag tcttatggtg cgaacatttt gattgaaccc
     6241 gccggacagg ctgcgctgcc cgcgctgttt agtgagagca gtaatccgct ttccggtcag
     6301 gattttcttg atgaagccga gctgccgaac atcaaagata tcttctggcg aaacaacatt
     6361 gtcgggtttg cgccgatgct cggcggcgaa gccagcgtgg agggagaacc agtgcggatt
     6421 ctgggcactt tcttcagtca gccagtggat attcctgatg aagagggata tgagacgggg
     6481 cagaaaacgg tcagcccata ctggcaggtg accggcgact ggccgcagga acccgcaggc
     6541 gcagagccgc agaccttagt cgggcacgcg ctggcgcggc aaatgggctg gaaacccggt
     6601 gataagctga cattacgtac cgaaggcgaa gcagtgcagg tcaccgtcag cggtatcctc
     6661 tccagcggcg gcgatgaaga taaccaactg gtgatgccgc taagcacggt acagcacctg
     6721 ctggggctcc ccggaaaagt gcaggcgatt cgcgtctcgg cgctgacggt gccggaaaac
     6781 gaactgtccc gccgcgcgcg ggaaaacctg gacgcgctca atgccgaaga gtacgacctc
     6841 tggtattgca cggcctatgt gtcgtcgatc gcgcatcagc tggaagaggc gatttccggt
     6901 gcggaagtgc gcccggtatg gcaggtcgcg gcgtccgaag gggtggtgat cgataaaatt
     6961 cagttgctga tggcggtcgt taccgtcgcg gcgctggtgg cttccgcaat ggggatcgcc
     7021 tcgctgatga ccagcaccat tatggaacgc gccaaagaga tcggtttgat gaaagcgctg
     7081 ggtgcgcgac agtggcaaat tatgctgctg ttctacctgg aagcggcgtc cagcgggctg
     7141 gctggcggtg cgctgggatg tattgccggg tgggggctgg cgaaagcgat tggcgtcatg
     7201 ctcttcgacg cgccgcttaa ttttgcctgg attgtggtgc cgtgcgtact ggtgattgcg
     7261 gtactgattg cgttgatcgg cacctggttc ccggcccggc ggattgcccg gctgtatcct
     7321 gtggaggtgc tgtatggccg ctaaacgtac catgctctgg ctgctggtgt ggcgggcgct
     7381 gcgtctgcgt tttcagcggg tcagcgtggt gtttgccgcg ctgatggtgg gggcgaccat
     7441 cgtgacggcg ctttttgccg tctggttcga cattaacacc aaaatgagcg aagaactgcg
     7501 caccttcggc gcgaattttt acatcgggcc ggggcacggt tccagtatgc cgcagcagga
     7561 gttgcagagc attctcgatc aggcgccgca ggggctggtg catggcgcca gtccgtggtt
     7621 gtacggcatg gcgcgtaccg agctggaaaa agtggtgatg gttggcgtgt ggttcgagtc
     7681 gctgcaaaaa ctggtgccgt actggcaggt caccggtagc tggattggcg ttagttttga
     7741 cgaccgtaat gcgatgatcg gcgtcaaact ggcggaacgc ctcaacgtgc agccgggcga
     7801 cagcatcacg ctggtggatc acaatcagcg taagaatttg cagattaaag gcattgtgga
     7861 gtctggcgat gcgacggaca acatgctgat cgtcagcctc gacgtggcgc aggcctggct
     7921 gcatcagccg ggcaaaatca gccacggctt gctgagcgtc agtaacgatg tcggccaggt
     7981 ggagaactat gccagccggt tgcaggcgca gtatcctgac ctggagattc gtccggtgcg
     8041 taaggtctct gcttccgaag ggcaggtgct ggataaaatt aaaggtctga tgggactggt
     8101 ctcgatcgtg attctggcgc tctcttcgct gtgcgtgaat accacgctga tggcgattgt
     8161 cggcgagcgg gcgcgcgagt ttgcgctgca aaaagcgctt ggttccagca acggcgatat
     8221 cgtgcggcaa attctgctgg aaaccagcat catcgcgctg gcggcggtgg cgtgcggctg
     8281 ggtgctggga tatttgctgg cgcaactgtt gggattaacg gtgtttaatg ccgccatatc
     8341 actgcgtctg ccggtgttgc ccattactct ggtgttgtct ttactggtcg ccattctggc
     8401 ggcgattgtt ccggtgcgac gcgcggtaag cgtcgagcct gctaaagtgc tgaaaggaga
     8461 gtagcgccat gtccgttacg catatccctc aggtggcgat cgaaacccgc cacttataca
     8521 aacggttcgg tgacgttacc gccctggatg acattaatct gcgcattgcg cagggtgaat
     8581 ttgtcgccat tatgggcgct tctggttctg gtaaaaccac gctgatgaat attctgacgt
     8641 gccttgatac ggcgacggaa gggcaggtgt ttcttgatgg caccgatgcc gccgcgctgg
     8701 atgaagaggg gcgccgtcgc tttcgcgccg agaaaatcgg cctggtattt cagcagttcc
     8761 atttgatccc gttcttaact gcgctggaaa acatcatgct cgcccagcat taccacagcg
     8821 tggtggatga agcggcggcg aggaaggtgc tggaacaggt agggcttggg catcgtgtga
     8881 cgcacctgcc gagccagctt tccggtggtg agcagcagcg tgtgtgtatc gcccgggcgc
     8941 tggtcaacga accgccggtt attttcgcgg atgaaccgac gggcaacctg gatgaagaga
     9001 atgagcagcg ggtgctggat ctgctcaccg atctgcatcg tcaggggcgc acgattgtga
     9061 tggtgacgca caaccctgcg cttgggcagt ttgccgaccg tattctgcgg ctgcaacacg
     9121 gtaaatatct gggtgaggag gccaatcaac atgcgctggc ataatgtgct gttactcgcg
     9181 gcggcgatcc tgataagcgg gtgtaaagaa gaaaagctgg cggtcggggc accggccccg
     9241 gcgctggcgg cgtttgatct gcaaggccag gagtcaggcc ttgaacgctg gcaaggaaag
     9301 gcggtgtacc tgaatttttg gtctgcggga tgcggggggt gcctagcgga aatggacacg
     9361 ctggaagcgc tgagcaaaaa atggggcgat aaagtggtgg ttgtggcggt caataccgac
     9421 cctgaaacgg tcaggatcaa cgatctgctg gcaaaacatc agataaccta tccggtggtg
     9481 cgcgatcagc tcaacattac ccaggaacgt tatcaggtga tcggcacgcc gacctccgta
     9541 ctgattgact ccgaaggacg cgttctggag ctacaccagg gtatgcgtaa gccgtctgag
     9601 ttagaggcca cgtttgcgcg cctggcggcg cgatgaacga cgcgtacgga gagataccca
     9661 atggcattta tctggaacga cgaaagcctt gcgattctgc gggaaaacgc gggcatcctc
     9721 acgacagagc agatagcgca actgcttcac actaatatta ccgccgtgcg gaatatggcc
     9781 tatcggctga aactgagcct tcgggtgacg gcgtataatc accgacgcat agcgcaggtt
     9841 caggcgcttt atgcctctga gacgttgagt ctcaaagaga tcgcggcgaa aacggggctg
     9901 acggccagta ccgtgcaata tatcgtgtac gtgaaatcaa aaaataagcc ctacgccacc
     9961 acggaatatg tctcttttga aacggagaac gccgttcatt accgcgtgca aaaagaattt
    10021 gtcgatacgg aacgttccct gctggacaac atctctgata acacgcgatt tcgtgaactg
    10081 tatctgacgg acggcacttt ttattgcgcc cgcaatatta agtatgaggt ttttatctcg
    10141 gagtaaccgg aacacgctga cctgccggtt atcttctgga atctcccgtt ttgcgaaacc
    10201 gttcccggaa aaatgtcata tttctgtcac actctatgag gattgataac aacaagaggt
    10261 tgtagggatg aaaaaattga tactcgccgc cgctgtggca ggggctgttt tattgtcgtc
    10321 cgccgcgcaa gcgcagacaa cgccggaagg ttatcaactg caacaagtgt tgatgatgag
    10381 ccgacacaac ttgcgcgcgc cgttggccaa taatggcagc gtgctggagc agtccacgcc
    10441 gaatcagtgg ccggagtggg atgtgcccgg cgggcagctc accacgaaag gcggtgtgct
    10501 ggaaatttat atgggccact acatgcgaga atggctggcg gagctgggga tggtcacgtc
    10561 aggcgaatgc ccgacgccgg acacggtata tacctatgcc aacagtctgc aacgcaccgt
    10621 tgcgacggca caattcttta tcaccggcgc tttccctggg tgcgacattc cggtacatca
    10681 tcaggaaaaa aatgggcacg atggacccaa cgtttaatcc ggtgatcacc gatgactctg
    10741 cggcgtttag ccagaaagcg gtgcaggcga tggaaaaaga gcgcagccag atgcagcttg
    10801 acgacagcta tcagctgctg gcgcagatga cggactacaa agattcaccg tcctgcaaag
    10861 aaaaacagca gtgttcgctg accgaagcca aagacgcctt tagggaaggt gcgaataagc
    10921 aggtcatgtc ttcccaagct gactcgttga ttaaaatttc gcggatctgg gccgattttt
    10981 gtcccgcaaa cacatcgaat cagcctattt aggctatttt ttccaccatt tctggtgtta
    11041 tttccggttt ttactgagat ctctcccact gacgtatcat ttggtccacc cgaaacaggt
    11101 tggccagggt gaataacatc gccagttggt tatcgttttt cagcagcccc ttgtatctgg
    11161 ctttcacgaa gccgaactgc cgcttgatga tgcgaaacgg gtgctccacc ttggcacgga
    11221 tgct
//
LOCUS       DAYPHN010000151        11208 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000151 DAYPHN010000000
VERSION     DAYPHN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.151"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..904
                     /locus_tag="ACG0BW_003837"
     CDS             <1..904
                     /locus_tag="ACG0BW_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFK7260742.1"
                     /translation="RGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSALE
                     EFLHTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVNP
                     TFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEYL
                     FEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVNN
                     GPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQEE
                     KFDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(938..1540)
                     /gene="cobO"
                     /locus_tag="ACG0BW_003838"
     CDS             complement(938..1540)
                     /gene="cobO"
                     /locus_tag="ACG0BW_003838"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFK7260743.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1550..3202)
                     /locus_tag="ACG0BW_003839"
     CDS             complement(1550..3202)
                     /locus_tag="ACG0BW_003839"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFK7260744.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGNEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDNGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3310..4590)
                     /locus_tag="ACG0BW_003840"
     CDS             complement(3310..4590)
                     /locus_tag="ACG0BW_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFK7260745.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4751..5068)
                     /locus_tag="ACG0BW_003841"
     CDS             complement(4751..5068)
                     /locus_tag="ACG0BW_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFK7260746.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5065..6435)
                     /locus_tag="ACG0BW_003842"
     CDS             complement(5065..6435)
                     /locus_tag="ACG0BW_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFK7260747.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALEFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDSEVYVSGSKHEETPYSLKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIHINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6439..7680)
                     /locus_tag="ACG0BW_003843"
     CDS             complement(6439..7680)
                     /locus_tag="ACG0BW_003843"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFK7260748.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7680..9125)
                     /locus_tag="ACG0BW_003844"
     CDS             complement(7680..9125)
                     /locus_tag="ACG0BW_003844"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFK7260749.1"
                     /translation="MELRNKKLTHNEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9144..10532)
                     /gene="glmL"
                     /locus_tag="ACG0BW_003845"
     CDS             complement(9144..10532)
                     /gene="glmL"
                     /locus_tag="ACG0BW_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFK7260750.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10532..10981)
                     /gene="glmS"
                     /locus_tag="ACG0BW_003846"
     CDS             complement(10532..10981)
                     /gene="glmS"
                     /locus_tag="ACG0BW_003846"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFK7260751.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGNILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFSPSHDLEDVCQLMAHDINQRHNVDTRILEEAI"
BASE COUNT         2680 a         2813 c         2808 g         2907 t
ORIGIN      
        1 gcgaggaaaa atacctaaaa cggagctgct ggtgactttt gaggtcgtcg cacgtcatga
       61 aagttatacc agagcggcag aggagttagc gttgacccaa agtgcagtat tcagacaagt
      121 gagtgcgctg gaggaatttt tacatactcc gttattttca cactctaaaa aacgtatttt
      181 tcttaatgat gcagggaagt attacctggg gatagtaaaa gaaacattaa ataagctgga
      241 acgagatacc aataccatta tgacctggca gcctacggtt caggttattg aacttgccgt
      301 gaatcctacg tttagtaccc actggttaat cccaaattta catgaattca caaagttaca
      361 tcccgatatt attgttaata ttcactcttt agctaataac ggcgattttc ttaatcggga
      421 gtacgatgct gtcattatgc gcgaaaattt ttgcgcgccc tgggcggaag tagaatatct
      481 ctttgaagag gagattttgc cggtatgcag cggcagtttg cttgcaatgt ccgatcaaaa
      541 actgtcagtg gcagaactgc ttacagaatt accacttctg catcaaagta cccgtattac
      601 tggctgggaa gagtggtttg ccttatctgg cgttagcagt ccgctagtca acaacggacc
      661 acgtttcgat ttgctttcaa tgctgattgc cgccgtgcgc tcgaatctcg gcgtggcgct
      721 gttaccacgt ttcgctattc aacatgattt ggatagtggc gatatggtga tcccctgtga
      781 tgtgcctatt cgtaccggta accgttttat tatgacgtgg caggaagaga agtttgactc
      841 accgcacttg cagcaatttc gtgaatggtt gctggcgaaa tcggttgtac cgcaggagat
      901 gtaaaacctg cgcctggcgg gatgccaggc gcagttatta ccagtcgatg cccggctggg
      961 cctgaatgcc gttatcaaaa gcatgtttga cggggcggat ttcgctgacc gtatctgcca
     1021 tttttaatat ctgactgtgg caaccgcgtc cggtgacaat gacactttgt tgtgccgggc
     1081 ggttttgcag agaagcgatc acttcttcgg tatccagata atggtaagcc agcatatagg
     1141 tcagctcatc aagcaccact aagtcgtaac gcttgtcagc cagcatccgt ttgctttccg
     1201 accagacctc tttcgccgcg tcgatatccg cttgccggtt ctgtgtctcc caggtaaaac
     1261 cggttcccat aatatgaaac tcaaccccga gcggctggag aaggttgtat tcgccgttgt
     1321 cccattgtcc cttgatgtac tgcgcaaccc ccaccgtttt gccatgaccc accgcgcgcg
     1381 tcacagtacc aaacgcggca gtggatttcc ctttgccatt gccggtgaaa acaatcaaaa
     1441 tccctttttt ctctatcgcc gccgcgacgc gcgtatcgac ctgttccttc aatttctgct
     1501 ggcgctgcac atgccgttca gttgaaattc gcgcttccat tcattttcct taatgtcccg
     1561 cagggcaact ggcgcagcgg tgttgctgct caaactgact aaagaaatta ttgcctttat
     1621 catctaccag gatgaatgcg ggcagatttt ccacttccat catccacacc gcttccatac
     1681 ccagctccgg gtattccagg caacgcagac ttttgacata ttcttgcgcc agtaacgcag
     1741 ctgcaccgcc aatacttccc agattgaagc cgccatgttt gtgacaggca tcggtgacct
     1801 gctggctgcg gttacctttc gataacatta ccagactgcc gccagcagcc tggaaggtat
     1861 cgacataacc gtccattcga ccgcccgtgg ttggacccag tgaaccgcag gccatgttct
     1921 ccggtgtttt cgccgggcca gcgtaataga caatatgatg cttcaggtaa tccggcatcg
     1981 gttcgccgtt atccagccgc gcttttattt tggcgtgggc gatatcgcgt gccactacga
     2041 tgggaccact taacgacacg cgggtgccta ccggtaatct ggcgagatcc tgcatcacgt
     2101 cacgcaacgg acggtttaga tccagttgta catgttgcgc gtgattttct tcccgcagtg
     2161 aggctggaat atattgcccc ggattgtgtt cgagtttttc cagccagata ccgtgcttgt
     2221 taatttttgc tttgatattg cgatcggcag aacaggagag cgccatagct atcgggcagg
     2281 agccaccgtg acgcggcaga cgaatgacgc gaatatcatg agcgaaatat ttacctccaa
     2341 attgcgcacc aataccaaat tgctgactgg cctcgagcaa tactttttcc agctcaatat
     2401 cacggaatgc ttgtccttgc tcgtttccgc tggtgggcaa attgtcgtaa tatttcgttg
     2461 aggccagctt ggcaactttt aaggtttgat cggctgaaag ccctccgacg acaaaggcga
     2521 tgtggtaagg cgggcaggca gcggtgccca gcgatttcat tttttcgatt aaaaatgcgg
     2581 tcagtttttc tggttgcaat aaggatttgg tttcctgata aagcgcggct ttattggcag
     2641 agccgccccc tttattaaca cagagaaaat ggtactcatt accagcgaca gcgctgatat
     2701 caatctgcgc cgggagattg gtttgcgtat tgacttcggt atacatatcc agcggcgcat
     2761 tttgtgaaaa gcgcagatta ttttcctgga aagtactgta aatgcctttg cttaaggctt
     2821 cagcgtcgtt acctcccgtc catatctgtt gccctttact ggcaacaata gttgcggtgc
     2881 cggtatcctg acagttgggt aatacccctt ttgctgagac ttcagcatta cgtaataact
     2941 gcaacgcaac gtatttatca ttactactgg cctgcggatc gttaagaatg ctggcgacct
     3001 gttgcaaatg agcggaacgt aagaaaaaag aggcttcata aaatgcctgc cgggcaagca
     3061 gcgttaatgc ttcaggagcg atttttatca cttcttcgcc gtctaattcc gttactgtaa
     3121 tatgttgatc gctaattaat gtatattctg taccgtcttt attttgtaga aacggttctt
     3181 gccagataaa tggtttggac atatttcact tttcacctta aacgttagtt ttacttaatt
     3241 gccgtctaac ttcatgatcc ggcttataaa aatgcgtagt cattggcttc tgattgctta
     3301 agatccccct cagggcagag caacgttcga ttcattgggt tttaatgctt taccccgaca
     3361 gccctcgccg aaatgttcgc cttcccaccg actcaccatc gcagcgccca ttgcgttgct
     3421 catgacgttt gtcgctgaac gccccatatc aaggaacgga tcaacgccca tcagcaagat
     3481 cagccccgct tccggaatat tgaactggtt gagcgtggcg gcgataacca ccattgaggc
     3541 gcgtggtacg ccagccattc ctttcgaggt caacatcagg atcaacagca tggtgatttg
     3601 ctcaccgatg gataaatgga tattgcaggc ctgggcgatg aaaactgtgg cgaaggagca
     3661 gtaggccatt gatccaacga gattaaatga gtagccaatg ggtaagacaa agctggcaat
     3721 tttgggggaa acgccaaatt gctccagttt ttcaagcgtt cccggaaaag ccgcttcaga
     3781 actggatgtg gtaaatgcca gcagggcggg ttccgaaagg gcgcgggtca ggcgtctgat
     3841 gcaggggcca acataaacga tggccagacc gataagcagc acccaaagta ataacatggt
     3901 gaaataaaat tcacccataa agatcccggc gctcaccata actgccagtc ctcgttcagc
     3961 aatcaatgct gaaatagcag cgaatacggt caggggagcg aagagcatga cgtagccagt
     4021 gagctttaac atggcatgtg ccagcgaatc taaggcgtga acgatggcgc tgcctttctc
     4081 gccaatcgcc gtcaggctac agccgaggaa aattgagaac accacgattt gcaaaatttc
     4141 attgtgcgcc atggcatcga caatgctggt ggggaaagca tgcgaaataa atacttttag
     4201 cgtaaagggt tctgacgcga ccactccggt ggtttcggct ccgtgtgcaa caaaattaat
     4261 tcctgtgcct ggcatgaaga aatttactgt tatcaagcct aaggcaattg acagcaatga
     4321 ggcgcaaata aataaaaaga gtgttttaga aaaaatacga ccaagggctt tggcatctcc
     4381 cattttagct atacctacca ccagggtaga gaccactaac ggagcgataa tcatttttat
     4441 cagtcgtaag aaaatcgtcg tgaatatcga tatttcttgc gcatagagtt ttgcggtttc
     4501 cggtgaagca gtgttattga gcactacgcc gataatcatt ccaagtacca gcgccaaaag
     4561 aatcatcgtg gttaaactta ttttcttcat tttttcctcc atcaggtgca ccgcgataaa
     4621 tcagaatcag gagaagtaga tgcccgaata gagattcagg catctacctg ctattacaac
     4681 cttacaaata gctgaagtgt tgaactgcaa taagtgacgg gatgtcagtt ggctaaaagt
     4741 gttcggattt ttacaaatcg aaactcatat ttaataaagg tgcatgttgc tgtgcgccat
     4801 agacatcatt gtcacctaca ttcccggaga taatatctcg tggcatgacg atttttactg
     4861 cgttagaagg atcaaaccag acaatacggt gaataaagtc aggttcaaca tgatataagc
     4921 gcgcgatcag ttctggggtt aattgccctg atgatttaac tcgctcatag ttttctttcg
     4981 ttttaaataa aatatctaaa accagttcgt aaggtccggc atttttcgaa cgaatgactt
     5041 gcgccagtga gacaattgac tgtttcatgc ctgaactcct tctggcgtca cctgttcaat
     5101 atggaaatca aaacgcagag cgtcgttggc ttcaatcagg tgatatatgg aaaattcgta
     5161 tactggcccg ccctggatat cagaagggga gaacgggaac gccaggttgc ctgcggtagc
     5221 gatgcggttt tcatagccgt agtgcagcat agtagagcgc accagcgagc aaacgctgtt
     5281 ggcaatctcc tgggtcggtg caactacatc aaggacaatc cccagctcat gcccggcagt
     5341 ttgcatcggt tcatggtcgc ccatcacccc gtttttgccg tacaggtgga aattgatgtg
     5401 aatgctgtca tcgtcgagcg acaggttacg cgaaacgctg gttttgactt catcgatgat
     5461 tttatcgatc ccggcgatca tgatcgggtc gcgcgttccg gcgatggtca gacagcggaa
     5521 gcccacccgt cgcgcacctt ccagtttcag ggaatacggc gtttcttcat gcttagaacc
     5581 gctgacgtac acttcgctgt cattgaccgc tttaaatgtg cagcctttca ggttcaacac
     5641 gccgccaggt ccaggcagga agtagggatc ggatttctca tacagtgtgt gtgcagccgc
     5701 tgacgtttcg gtaaatttac gcttcggatt aaatgtcttc agcgtaaagc cgttgtcatc
     5761 aatgatgccc atcgcacagt cagagcctga gccgggcgtt gcggcgattg ccgcgcattc
     5821 aaggattttc ccacaatgca gcgccagacc ttcatcaaag ccttgcatga tcggaagcgc
     5881 agcaaagcag gccgggtcgt aagcgcgccc gcccagcacc acttgcgcgc cttctttcag
     5941 cgcccgctgg aagggttcga tgcccatttg cgcaacgata tacgtacttt cgtcaatcgc
     6001 gtcgtgggtt aacggcggga caaactccag cgccgtgatt ttgccgttat ccagcgcctg
     6061 gtggacgatg gctttgtcaa cgtctgccgg gatcagcgcc attgagaacg agaggtgctc
     6121 ttcctgggca atctcatgaa ttatttctcg gcaccactcc aggtgtggtg cggcaccaga
     6181 accgccagcc gttccgatca ccaccggtat attattttgc acgcctgcgg tgatcatata
     6241 gcgcagatcg cgcttgaccc ctgcgcgatc ggtaaacggt ttgcctgccc cgaggtagtg
     6301 ggggcctgga tcggaagagc ctgcatcaac ggcgatcaga tcgggcgatt ctgccatcgc
     6361 tttacggaag ctctcttccg ggaatccata cccgaggatt gccgttggcg ataacacttt
     6421 aaaagaacga gccatttgtt aatccttagc ctgcaacagc gcgatagtac gattcatttc
     6481 gttgaagacg atatcgaggc cttcgtcaaa gcccatccct ggctttacca gcatacgcat
     6541 agggcgagcg gcaagggcga cgtggacaca ggtgcgggca ctgacatcag tttcattgca
     6601 ggtgccgccc tggtacgctt ccatgctgtg gctattgcag taaagaaccg catcgacgat
     6661 gttgtgaata ctgcccagat ccggggtttt gatttgcacc atgtgacaac tggcggcatc
     6721 agtaaaatca acaatatcct ggtaggtgtt acaccattca tcggccacaa ttttcacgcc
     6781 ggaaccgagg cgcgtcagct ctttggtaat cgcggtcagc aggcgaattt gatcgggctt
     6841 gttaccggca tcgaccggcc cttcaatgta gagcggcagg ccttgcgctt ctttttccag
     6901 gctggcgatg tattgcgcac agcgaagcgg atccatatcg aagatcagac cgatagtgcc
     6961 gtatacatcg atgtgcaggg tagggtggta gcgtgcgctg gtgcgcttgc tcagaatacg
     7021 atccgacagc cagcggacat attcgcgcag tttttcacct ttaaagccta gtttctcttc
     7081 gacgttatta atcagtgcat ggggcagcac gtcgatgccc ttaaggatca tcttatcgac
     7141 ggcgatatat cgatcgtcac cgctctgacc aaataatgga atggattccg ccacgcgtgg
     7201 caactgccat tcatcgcaaa ctacttcagt tttcagacgg ccggttgcca gcgcggtagc
     7261 atcaagcagc gcctgtgata atccgtagcg cacggcggta tgcagcaagt tgccgtcaat
     7321 acgcaatttg tcgaagaaac gggcattcgg caggaaagca tccacatcgc ggcctaccag
     7381 taatggcttg atatggtcgt tgagaaacgg aataaagtgc tcagcgagga acagtggatc
     7441 gcgaccacct gccccggaat actgtacggc agcacagtca cctacggcga ccgcgccgtt
     7501 ttccagaatc aactgtaccg atacgcactc cccggcctga cgaacggcgt taaacccctg
     7561 tgttaccggc tccccggtat aaaaaaagcc gtcatgaccc gctccgtttt ttatcgcctg
     7621 ctggtcatcg aaatagaatg aggagtagcc agcggtgaac agggcctgtt ttattttcat
     7681 tatggtcttc ctattaattt actgtgggat acagcattga tgtcatctac aaccatctgg
     7741 aatgtcggtt ggcgtccttc aaagcgtgcg cgttcagcaa cgtaatcgtg gtggaggtcg
     7801 agaatatctt ttggcaatgg aacggctcca gcttccagta cgcgtatcgc gccggtgttg
     7861 tcacggaccg gaagaatttt gcccgcgtta cacgcggctg gtgcaaacgg cacatccagt
     7921 acgcccgctt caaatgccag caccgtaccg cgcgcaatat caccattacc cagttcgaac
     7981 actttattca gtactgcgcg aacctcgctc ttaatcagtt caatttccag ttcaaccgca
     8041 gggcacggcg ggaatttctg ctcgttgacc atgttgagca tctggcggga ggctttcagc
     8101 ccctgaatgt tggcagcggc agtcgggata ccccaggctt cgtgcgggct tttggtgatg
     8161 actttggtgg caccagacat ccccgcgacg gctgcgcccc aggagataat agcgaacgct
     8221 ttcgattcgt cttccggaaa gccgcccatc cactggtgaa acacggtact taattcatag
     8281 tcggtatagc catagctctg aaaatattcg tgtgcaagtt cacgcagaga ctggatcgcc
     8341 gcaacatcct gcgtcaggct gccgacctgt ccataaccaa cggtgataga tttcacaccc
     8401 tgttccagcg ccagcaagcc ttcgattatc gcgatagagt gcgagataaa cggcgggatc
     8461 agcgtacccg ttagtgggcc aaacggttcg cggttgatgc ggatcccgtg ttcttcatac
     8521 atccccatta aacggtcgca gtactgccag tcgcggatcg atttttccag cgttacgcgt
     8581 ttggcatacg gaatattgta ggaaatgccg ccgccttcat aactggtaaa gccgctggcc
     8641 atcgagattt cagccagcag acgcgcatct ggcgtgccgt ggcggatctg tagcggtttt
     8701 tgcagagttt cggtgaggcg acggcaagct gcgacgccgt ggttaacgac cggtaaaccg
     8761 ttcagtttag aggttccggc ttcgatggat tttttaatcc ctacagcagc ttcttcgtac
     8821 cgattcagac gggtatatgc atcgatggtg ctcggcagca gatcgcactc ttcttgtagt
     8881 gttttcagta gttcgatgtg ttcgtccatc agcgccacgc ctgcgcgcgg ttggctaaga
     8941 gttttacctt ccttgtctgc tttcagcagg gcaagagaga agcgtttatg ctcaggaatg
     9001 gtttgttggt atttaacgcc atcttcaaag ttttcaacat ccttgccggt ttcccaggtt
     9061 ttcaatacct gttgccgctc ggtcatgaat tcgttatggg ttaatttctt atttcgaagt
     9121 tccattgatt gcagccttaa ttgttatagg gttaagcatt gaatactggt gcgcgcggcg
     9181 gccagcggat cgactctggc gacgtttgcc agcagtggta atagcccccg ggaatcgcgg
     9241 taatattcaa attcggtcgg taagagaatg cgtttaccgt cgtcgtttaa ctcgcggtat
     9301 ttcaaccagt gatgcatatc gaactgactg gcacgcgaga gccatccgcc ggatcccacg
     9361 actttacgca cggttgtcag gtctcgtccc atctgtaaat cgacattgcc gacgcaggtg
     9421 cagacctctt ttttggtgcc cgcatgtctt tcggtggcat aacccacaca cagtccggcc
     9481 aggacggtat cgaaatattt ttcttctgca ttcgcgggta agtagtcggg atgagcgact
     9541 agatggcgga ggtaatgata aaaagcgtcc agctgtgcgg gttgctgagc aaaattcaca
     9601 ttgactaact ctttcgcact ttcaccaacc accattgccg agacgcgcat tcccagatcg
     9661 ccttccaccg tccgttttac gaagggttcc ggtacgccat gtaaaacagt gtcgggcgag
     9721 agcgtgttgg cacaggcgga atagacatcg gtggttgcgc cgcccatgtc gatcagcatg
     9781 aactctttcc agctgtgatc gacgttactg attgccttaa ccagttcaaa gaccgtccat
     9841 ggtgtcggca ttggttcttc gcctgtttta tcaacaatga catccagccc tttcccttta
     9901 acgatgcgtt taaggaatat gtcgcagata gcctggcgtg cagctaaagg gttcggatgg
     9961 tcaagatcgg gcagaacgtt gtcgacgatg gtcaggtctt tatgcccgag gatctcctgc
    10021 acctcgtcct gaatatcccg gtttccggca taaataatgg cgcagtcgag tttggattct
    10081 gccaacacac gggcgttatt cagtccgtag ctttcttctc cgccatcggt gccgccggtg
    10141 aataagagaa tatcgggctg cgtttcttcc agtgcctgga tatcacggcg gttcagcttg
    10201 taggcgtaat attgcgcaat tttagcaccc gcagagtgag cggttacttt cgcggtttcc
    10261 agggtgatag aaggcaccaa tcccattgcg gcaaccgcca gcccaccttt ggcagaggag
    10321 gaatacttca atgcaacctc accactgttt aacaggggga gggcatcatc tacgtttaac
    10381 acctggtcca ggctggagaa aaaccccttc gccagatgat gggtggtggt tggggtcaga
    10441 acgtggttaa ccaacgttaa cgcgtccccc tcccgggtga agagggctgc tttggtccac
    10501 gtcgaaccaa tatcgacaga gacgatttgc atcagattgc ctcttcaaga atgcgagtat
    10561 caacgttatg acgttggttg atatcgtggg ccatcagctg acaaacatct tctaagtcat
    10621 gacttgggga aaagacgcga tcaaatccca tctctttgaa cttggtttcc acgtcagcga
    10681 agtcatgttt tccgaccacc aggttaccgc ccacatagag caggatgtta cccagcccac
    10741 gctcgatgca gcgttcgcgc atgcccagac aatcgatatc gccatgccca tagatagagg
    10801 agacgacaat cgcgtcagca ccagtttcaa tggcagcatc aatatattcg tcctggctga
    10861 ccattacgcc cagattaata actctgaagt catggttgct aaaaacgcgg tccagaactt
    10921 tattgcctac tgcatggcag tccgcgccaa taacgccaat cacaagtgtt gctttcttca
    10981 tgggtaatcc tttgtaagac agagagtaaa ataagttaca cacgtcgtat ttatggccgc
    11041 tcataacggc acaggtttaa tatcacagga gtgtctttat ctgaccgtaa tgttgcaatt
    11101 gatctatttt caaaatgtct ttcgggaaat gattgtttaa agtgtgtttt caaattctca
    11161 ttttaattca gtgtgttatg tcaactaatg tcatgaatgt taaatttt
//
LOCUS       DAYPHN010000152        11186 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000152 DAYPHN010000000
VERSION     DAYPHN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.152"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(239..2230)
                     /gene="tkt"
                     /locus_tag="ACG0BW_003847"
     CDS             complement(239..2230)
                     /gene="tkt"
                     /locus_tag="ACG0BW_003847"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFK7260752.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2508..3266
                     /gene="loiP"
                     /locus_tag="ACG0BW_003848"
                     /gene_synonym="yggG"
     CDS             2508..3266
                     /gene="loiP"
                     /locus_tag="ACG0BW_003848"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFK7260753.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQEAEADDYSYDLLRQHGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3352..4914)
                     /locus_tag="ACG0BW_003849"
     CDS             complement(3352..4914)
                     /locus_tag="ACG0BW_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFK7260754.1"
                     /translation="MERTLPWKPLLCLLFLLSTGCDRAEKQKIAAQLQQDKEAISSVV
                     KKSYRALCGPEFVISTDSHDRLAEMQQVFTPALMEPVLKYWQESPKTCKRRFPSYSDP
                     LATFVEGDKAQIGAFFGHQSDIDIPMELIREGDGKWKIAAIDFASLHEFEKNGRVTDE
                     VQKKIDLLKKRSFKQRRDFPLLTRTEPERVSKSPLTSVEQTVTEPDSSWLDVLLTIGK
                     YALYLGAIILILALVAVLWIGYGLVFAGHRLFKASCKDPSIQKIPAAERWAMAVGAPY
                     AIAGNNHWARRVVNDDSVEAENDCKSEVESLADMWGVFDRDSLLEQLLALFVAGHRSV
                     YEEQIKNDSEMPDAEYRAFAQQLAQNAKSSSEAKERLWQLQMVRKNRRKICDIDFCAW
                     DMVRFVMLCNSGAQVGYITQREMVDFSMLAARRVQQHYRSWRELAGHFLLARWYWKAT
                     DKRHLITHILFKKAITSLLKEQGSPWRTLPWDTPLPEGTEAAFAWACGVSTADVVEVR
                     SDTPDKAIYEAN"
     gene            complement(5064..5984)
                     /gene="speB"
                     /locus_tag="ACG0BW_003850"
     CDS             complement(5064..5984)
                     /gene="speB"
                     /locus_tag="ACG0BW_003850"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFK7260755.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6120..6851)
                     /locus_tag="ACG0BW_003851"
     CDS             complement(6120..6851)
                     /locus_tag="ACG0BW_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260756.1"
                     /translation="MKKGKVRSALVALVVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRF
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPTQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6997..8973)
                     /gene="speA"
                     /locus_tag="ACG0BW_003852"
     CDS             complement(6997..8973)
                     /gene="speA"
                     /locus_tag="ACG0BW_003852"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFK7260757.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8982..9113)
                     /gene="yqgB"
                     /locus_tag="ACG0BW_003853"
     CDS             complement(8982..9113)
                     /gene="yqgB"
                     /locus_tag="ACG0BW_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFK7260758.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(9207..9350)
                     /gene="yqgG"
                     /locus_tag="ACG0BW_003854"
     CDS             complement(9207..9350)
                     /gene="yqgG"
                     /locus_tag="ACG0BW_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFK7260759.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(9461..9712)
                     /gene="yqgD"
                     /locus_tag="ACG0BW_003855"
     CDS             complement(9461..9712)
                     /gene="yqgD"
                     /locus_tag="ACG0BW_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFK7260760.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            9768..10922
                     /gene="metK"
                     /locus_tag="ACG0BW_003856"
     CDS             9768..10922
                     /gene="metK"
                     /locus_tag="ACG0BW_003856"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFK7260761.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2799 a         2978 c         2764 g         2645 t
ORIGIN      
        1 cgcgcacttt gccaataatc tgaagggccg ctttcgcggc ccttgagact gatggcaaag
       61 gcaaaactgc ctgatgcgct acgcttatca ggcctacatg cttcctgcaa tatattgaat
      121 ttgcacgatc ttgtaggtcg gataaggcgt tcacgccgca tccgacatga acaacgcgca
      181 ctttgccaat aatctgaagg gccgctttcg cggcccttct cacaaagcat cttaccgatt
      241 acagcagttc ttttgctttc gcaacaacgt tgtcaacggt gaagccgaac tcttcaaaca
      301 gcagctctgc cggagcagat tcaccaaagg tggtcatgcc gacgatagca ccgttcaggc
      361 caacatactt gtaccagtag tcagcaatgc cagcttccac cgcaacgcgt gcagtaaccg
      421 ctttcggcag tacagactca cggtaagccg catcctgctt gtcgaatgcg tcagtagacg
      481 gcatggaaac cacgcgcgct ttcacgcctt cggcagtcag tttttcgtag gcagcaaccg
      541 ccagttcaac ttcagaaccg gtcgcgatga aaatcagttc cggctgaccg gcacagtctt
      601 tcagcacata accaccgcgc gcgatgtttg ccagctgctc ttcagtacgt tcctgctgcg
      661 ccaggttctg acgggagagg ataagcgcag ttgggccgtc ctgacgctca acgccgtatt
      721 tccacgcgac cgcggattca acctggtcac atggacgcca tgtggacatg ttcggggtca
      781 cgcgcagaga agccacctgc tctaccggct ggtgagtcgg gccatcttcg cccagaccga
      841 tggagtcgtg ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc atacgtacgg
      901 cgttacgtgc gtattccacg aacatcagga aggtggaggt gtacggcagg aaaccaccgt
      961 gcagggagat accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt
     1021 agttacccgc agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg
     1081 gtgccaggtc agcagagccg ccgaggaatt ccggcaacag cggaccgaac gcttcaatcg
     1141 cattctgaga cgctttacgg ctggcgattt tcgccggatt agcctgcagt ttagcgatga
     1201 attctttcgc tttggcgtcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt
     1261 cagcggcttc ctgcggataa gctttggcgt aagcagcgaa tttctcgttc catgcggatt
     1321 ctttcgcctg gcctgcttct ttcgcatccc actgagcata gatttcagac gggatttcga
     1381 acggcgcgta tttccagccc agctgttcgc gggtcagggc gatttcagcg tcgcccagcg
     1441 gcgcaccgtg ggagtcgtgc gtaccggctt tgttcgggga accgaaaccg atgatggttt
     1501 tgcacatcag cagggacggt ttgtcagtca ctgcgcgcgc ttcttctact gcgcgtttga
     1561 tggatgccgc gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtag gcttcgaaac
     1621 gcattgcggt gtcgtcggtg aaccagcctt caacgtgacc atcgatagaa ataccgttgt
     1681 catcgtagaa cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt
     1741 gagagatgcc ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacga
     1801 tgtcgtggcc cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccga
     1861 ctgcgttggc aataccctga cccagcgggc cagtggtggt ttccacacca gcggtgtaac
     1921 ctacttccgg gtggcccgga gttttagagt gcagctgacg gaagtttttc agttcttcca
     1981 tcggcagatc gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt
     2041 tggacagcac gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgcttcagga
     2101 aatcacgcca caggacttcg gcaatgtcag ccatacccat aggggcaccc gggtgaccgg
     2161 atttggcttt ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac
     2221 gtgaggacat tttgactcca gatcggatga tgaagggcac gcccttaacg acttgacgac
     2281 agcgcgtttt gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg
     2341 tacatggaac atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa
     2401 tctgctcgcc cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca
     2461 aacggcgcat ttttagaata atcctgacct tgtgcggaag agaaaacatg aaaattcgcg
     2521 ccttattggt agcaatgagc gtggcaacgg tactgaccgg ttgccagaat atggactcca
     2581 acggactgct ctcatcagga gcggaagctt ttcaggctta cagtttgagt gatgcgcagg
     2641 tgaaaaccct gagcgatcag gcatgtcagg agatggacag caaggcgacg attgcgccag
     2701 ccaatagcga atacgctaaa cgtctgacaa ctattgccaa tgcgctaggc aacaatatca
     2761 acggccagcc ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc tttgcaatgg
     2821 ctaacggctg tatccgcgtc tatagcgggc tgatggatat gatgacagat aacgaagtcg
     2881 aagcggtgat cggtcacgaa atggggcacg tggcgttagg ccatgtgaaa aaagggatgc
     2941 aagtggcgct tggtacaaat gccgtgcgag tagctgcggc ctctgcgggc gggattgtcg
     3001 gcagtttatc gcaatcacag cttggtgatc tgggcgagaa attagtcaat tcgcaattca
     3061 cccagcgcca ggaagcagaa gccgatgatt attcttacga tcttctgcgc caacacggca
     3121 tcagcccggc aggtcttgcc accagctttg aaaaactggc gaaactggaa gaaggccgcc
     3181 aaagctcaat gtttgacgac catccagcat ccgccgaacg cgcccagcat attcgcgatc
     3241 gtatgagcgc ggatggaatt aagtgatttc atatttctcc gacaggaatg tcggagaaat
     3301 tttcattcag ggccggccac agtcgttaaa taaactcaat catttggggc atcaattcgc
     3361 ttcgtagatt gctttatccg gtgtatccga tcgcacctct acgacatcgg cagtgcttac
     3421 gccacaagcc caggcaaatg cagcctctgt cccttcaggt aatggggtat cccagggtaa
     3481 agtacgccac ggactcccct gctcttttaa taaagatgta atggctttct taaaaagaat
     3541 atgggtaatc aggtgccgtt tatccgttgc tttccagtac caacgggcca gcaaaaaatg
     3601 cccggccaat tcgcgccagc ttctgtaatg ttgctgtacg cgtctggctg ccagcatcga
     3661 aaaatcgacc atttcacgct gggtaatgta tcccacttgc gccccgctat tacatagcat
     3721 gacaaaacgc accatatccc aggcacaaaa atctatatcg catattttac gtcgattttt
     3781 tctgaccatc tgtagctgcc acaaacgctc tttcgcttcg ctacttgatt tcgcgttctg
     3841 agctaattgc tgagcgaacg cgcgatattc agcatccggc atttcgctat cgtttttgat
     3901 ctgttcttca taaactgaac gatgccctgc tacaaacaaa gctaagagtt gctcaagtaa
     3961 cgaatcccga tcaaagacgc cccacatgtc agcaagtgac tcaacttcac ttttgcaatc
     4021 attctctgct tccacggaat catcattaac gactcgtcgc gcccagtgat tgttacccgc
     4081 aatggcataa ggcgcgccga ctgccattgc ccaacgttcg gcagcaggaa ttttttgaat
     4141 tgagggatct ttacaggatg ctttgaaaag gcgatgtccg gcgaacacca ggccataacc
     4201 aatccacaaa acagccacca aggcgagaat aagtattatc gctcccagat aaagggcata
     4261 tttgcctatt gtcagtaaga catccagcca ggaagagtct ggctccgtaa ctgtttgctc
     4321 caccgatgtc aaaggggact tactgacccg ctcaggctcg gtcctcgtca acagagggaa
     4381 atccctacgt tgcttgaatg agcgtttttt aagcaaatct atttttttct gcacctcatc
     4441 agtaacccga ccgttttttt caaactcgtg taacgatgca aaatcaatcg ccgcaatctt
     4501 ccatttacca tccccttcac ggattaattc cattggaata tcaatatcag attggtgacc
     4561 aaaaaaagca ccaatctgcg ccttgtcgcc ttcgacgaag gtcgctaaag gatcgctata
     4621 actggggaaa cgtcgtttac acgttttggg tgactcttgc cagtatttca gtacaggctc
     4681 cattaaagca ggcgtaaata cttgctgcat ttccgcaagg cgatcgtgcg aatctgtgct
     4741 gataacaaac tcagggccac ataatgcgcg ataactcttt ttgacgacag aggaaatggc
     4801 ctctttgtct tgttgcagct gtgcggcaat tttctgtttt tctgctctat cgcagccagt
     4861 tgataaaaga aataacaaac aaaggagggg cttccaggga agagtgcgtt ccatctattt
     4921 attccattaa aaacgcgata ataacaatcc attaaaaaac agcagtcagg gtaaagatga
     4981 cttaattaac aggataggtt ttaaatgata agtcccttgt gccagacaag ggactgtgca
     5041 aaaaattcaa tcaggcaggt tacttactcg ccctttttcg ccgcctggat atacagcatc
     5101 tccagcgcca gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc
     5161 acttccacta catccatccc aacgatgttg agatctttca ggccgcgtac cagtttaatg
     5221 gcgcgatcag aggtcaggcc gccaattacc ggcgtaccgg ttcccggcgc gaaagcagga
     5281 tccaggcagt cgatatcaaa ggtcagataa accggcatat cacccacaat ctgtttcacc
     5341 tgggcgataa cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
     5401 ccgttgtctt tatcgaactc agtacgaata ccaatctgca cggaatgatt cgggtcgatc
     5461 agaccttctt tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttcgca
     5521 taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga
     5581 gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttacct
     5641 gcagccagca gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg
     5701 aaggcatata ccagatcgcc gcagtccacg acgttcaggc gctcacgcat gtcgaaattc
     5761 cacgggaagc gattgtgttc ccaggccaga ttcgtcgaaa cctgacggat agcagccgga
     5821 ccgtggcgtc cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc
     5881 cagtcagcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggcg
     5941 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
     6001 caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt
     6061 gcgaaacaca agtttgtgct aattatcgcc ttaaatggac gaggcatcaa gcgaggttgc
     6121 tatttccgac acggtggcgg cgcatagcca ttgttatctg ccggaaaggc ttgtccagag
     6181 cacggcggca gcgcgttttg cgtcggacct tgtaattcaa aggcggcaaa gccgattacc
     6241 ccggtattac gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg
     6301 aactgaaaag ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taatgaatct
     6361 ccggcattca caatatagcc gttgttattc agcgaacctt gcttgccgtt cagcacatcc
     6421 aggccatcaa ccgtagcaac gatctcgtag ttggtgttac gactgtaatt acggacatat
     6481 aattgataat tcattccgac tctggcatgg acaatatagc gtggttcatt attggatgaa
     6541 tcataaaaac gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt
     6601 tcgttaatac gaatgctata aacgcggtca tagccggaag gaacattagt cgaataacta
     6661 ataacgatca tctctgccgg atcgcgccag gctcgctcag cagatacgcc ctgaaccgat
     6721 gagtgtacat ccccacccca ggcggtgccg acattgcctg atgcactgct attatactgc
     6781 gcattactgg aacaaccggc cagcagcacg actaatgcca ccaacgcgct acgtactttc
     6841 ccttttttca taaccgttcc ctctgcattt aattaatgca gcgattgtag ggagattatt
     6901 taataagaaa agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta
     6961 cgtaaataaa ccgacccgcc agcggaataa tcttaattat tcatcttcaa gataggtata
     7021 accgtacaaa cctgcctcga actcttccag gaactgttgt tgcagttcag catcaagatc
     7081 ggttttcttc acttgatcac ggaactgggt taacaacgtt ttcggatcga gctgtacata
     7141 ttgcagcata tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctgccgtc
     7201 agggaagacg aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tgttgccgag
     7261 gatctcctga tatgcgccga ccatgaagaa accgagcatc ggcggattct ctggatcgta
     7321 ctccggcatt ggcattgtcg tggcaatacc atcaccatca atatagtgat caatagcacc
     7381 gtcagagtca caggtaatat ccagcagcac agcgcggcgt tccggcactt gatccaaccc
     7441 ttccagcggc agaaccggga acaactggtc gatcccccac gcatccggca tcgactggaa
     7501 cagtgagaag ttgacgtaca ttttgtctgc catacgttcc tgcagctcgt cgataatcgg
     7561 acgatgagca cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcaggta
     7621 gagttgttct gcccatgctc gttcttgcag gctaaaagtg ccggaagagt agccgatatg
     7681 aatatcgtgc agatccatct ggctatcgtg taaccattca cgcagagaac ggcgagttcc
     7741 cggttcgtgc atctcctgcc aggtttccca catgctttgc agggcgcgcg gtgcgtcttc
     7801 tgcaggcgcg gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tcgacaccag
     7861 cacggtgtga tgcgcagtca ccgcacgacc tgactcggtg attaccgtcg gatgtggcag
     7921 accattttct tcacacgcat caccaatcgc ccagatgatg ttgttggcgt attcattgag
     7981 gccgtagttc accgaacagt cggactgcga acgggtgcct tcatagtcca cgcccagacc
     8041 gccgccgacg tcgaagcact gaatattgac gcccagcttg tgcagctcaa catagaaacg
     8101 cgcggattca cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg
     8161 gaagtgcagc agttgcaggc tgtcgagacg cccggcttca cgcagcgttt caaccagttg
     8221 cagtacctga gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt
     8281 acccgagccc tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg
     8341 ttctgcttca tccagcacaa tggcaatttc tgacatcttc tcaatgacca gatagacctt
     8401 gtggcccatc ttctcgccaa ttaatgccag gcggatatat tcgcggtcct tataaccgtt
     8461 gcagacgatg acgctacggg tcatgccagc atgtgccagc accgccatca actccgcttt
     8521 ggaaccggct tccaggccca gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg
     8581 gtgctggtta actttgatcg gataaacgag gaagtaatcg ccgttatagc cgtaggattc
     8641 ccgcgcacgt ttgaacgcgg cgttaataga acgcaaacga tgctgcagga tctgtgggaa
     8701 acagaacagt gcaggcagac gctggccctg tgcttcacga gttttcacta actgcgcgag
     8761 atcgacgcga gcttccggga cgtccgggtc cgggcacacg ctaatgtggc ccagctcgtt
     8821 aacgtcatag tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc
     8881 ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga
     8941 cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt
     9001 gtaaaacagt taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga
     9061 ttttccagca gtgaatgctg acgctccaac tgcgcaaccg gtttcttttt cataacatta
     9121 ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt
     9181 tttacaagag cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa
     9241 gcgccgagat gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac
     9301 gaatcttcac cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat
     9361 cacctccacg gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc
     9421 tggaagtggc aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt
     9481 tataccccac aatggcagaa aattgcaaaa gataaatacg cagaatgcca gcattgtcag
     9541 gaaaaatttc cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc
     9601 gggtgaagaa ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac
     9661 tcagactgcc agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc
     9721 cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc
     9781 tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatttctg
     9841 atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct
     9901 acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca
     9961 tcgaagagat cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct
    10021 ttgacgctaa ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc
    10081 agggcgttga ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg
    10141 gctacgcaac caatgaaacc gacgtgctga tgccagcgcc tatcacctat gcacaccgtc
    10201 tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg
    10261 cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggtatc gatgctgtcg
    10321 tgctttccac tcagcactct gaagaaatcg accagaaatc gctgcaagaa gcggtaatgg
    10381 aagagatcat caagccgatt ctgccggctg aatggctgac ttctgccacc aaattcttca
    10441 tcaacccgac cggtcgtttt gttatcggtg gcccgatggg tgactgcggt ctgactggtc
    10501 gtaaaattat cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg
    10561 gtaaagatcc atcaaaagtg gaccgttccg cagcgtacgc agcacgttat gtcgcgaaaa
    10621 acatcgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg
    10681 tggctgaacc gacctctatc atggtggaaa ctttcggtac cgagaaagtg ccttctgaac
    10741 aactgactct gctggtacgt gagttctttg acctgcgtcc atacggtctg attcagatgc
    10801 tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgcgaac
    10861 atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt
    10921 aatctttctt cacctgcttt caaaggccag cctcgcgctg gcctttttct tttacctcac
    10981 cgcggtggat aggcgttcac gccgcatctg gcaaaaagct gcccacacaa taacatcatt
    11041 cttcctgaac acgttttacc gcaaattatc atcgctactg aaaccgatta caccaaccac
    11101 accagacaaa gatttgtaat attttcatat tattattcgg ttttcacagt tgttacattt
    11161 cttttcagta aagtcttaat tgcaga
//
LOCUS       DAYPHN010000153        11146 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000153 DAYPHN010000000
VERSION     DAYPHN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.153"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..76
                     /locus_tag="ACG0BW_003857"
     CDS             <1..76
                     /locus_tag="ACG0BW_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260762.1"
                     /translation="SRSRGNRRWYTTGPVGLLTRPFIK"
     gene            133..1125
                     /gene="proX"
                     /locus_tag="ACG0BW_003858"
     CDS             133..1125
                     /gene="proX"
                     /locus_tag="ACG0BW_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFK7260763.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            1578..2762
                     /locus_tag="ACG0BW_003859"
     CDS             1578..2762
                     /locus_tag="ACG0BW_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7260764.1"
                     /translation="MTKTNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            2886..3623
                     /gene="ygaZ"
                     /locus_tag="ACG0BW_003860"
     CDS             2886..3623
                     /gene="ygaZ"
                     /locus_tag="ACG0BW_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFK7260765.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVSALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            3613..3948
                     /gene="ygaH"
                     /locus_tag="ACG0BW_003861"
     CDS             3613..3948
                     /gene="ygaH"
                     /locus_tag="ACG0BW_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFK7260766.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            4039..4569
                     /gene="emrR"
                     /locus_tag="ACG0BW_003862"
     CDS             4039..4569
                     /gene="emrR"
                     /locus_tag="ACG0BW_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFK7260767.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            4696..5868
                     /gene="emrA"
                     /locus_tag="ACG0BW_003863"
     CDS             4696..5868
                     /gene="emrA"
                     /locus_tag="ACG0BW_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFK7260768.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            5885..7423
                     /gene="emrB"
                     /locus_tag="ACG0BW_003864"
     CDS             5885..7423
                     /gene="emrB"
                     /locus_tag="ACG0BW_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFK7260769.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7487..8002)
                     /gene="luxS"
                     /locus_tag="ACG0BW_003865"
     CDS             complement(7487..8002)
                     /gene="luxS"
                     /locus_tag="ACG0BW_003865"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFK7260770.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(8152..9708)
                     /gene="gshA"
                     /locus_tag="ACG0BW_003866"
     CDS             complement(8152..9708)
                     /gene="gshA"
                     /locus_tag="ACG0BW_003866"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFK7260771.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSKELACTRVNWNRVILEGRKPDLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(9781..10209)
                     /gene="yqaA"
                     /locus_tag="ACG0BW_003867"
     CDS             complement(9781..10209)
                     /gene="yqaA"
                     /locus_tag="ACG0BW_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFK7260772.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(10206..10772)
                     /gene="yqaB"
                     /locus_tag="ACG0BW_003868"
     CDS             complement(10206..10772)
                     /gene="yqaB"
                     /locus_tag="ACG0BW_003868"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFK7260773.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(11052..11128)
                     /locus_tag="ACG0BW_003869"
     tRNA            complement(11052..11128)
                     /locus_tag="ACG0BW_003869"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Arg,seq:acg)
BASE COUNT         2627 a         2961 c         2822 g         2736 t
ORIGIN      
        1 ctcacgcagt cgcggcaacc gtcgctggta caccactggc cctgttggtt tgctgacccg
       61 cccattcatt aagtaactct gcacttgccc ggtaacgccg ggcatatcac cctgccaaaa
      121 aaaggaataa caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct
      181 acacaaactt ttgctgccga cctgccgggc aaaggcatta cagttaatcc agttcaaagc
      241 accatcaccg aagaaacctt ccagacactg ctggtcagtc gtgcgctgga gaaattaggt
      301 tataccgtca ataaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc
      361 ggcgatgcaa ccttcaccgc cgtgaactgg acgccactgc atgacaacat gtacgaagct
      421 gccggtggcg ataagaaatt ttatcgtgaa ggagtatttg ttaacggcgc ggcacagggt
      481 tacctgatcg ataagaaaac tgccgaccag tacaaaatca ccaacatcgc gcaacttaaa
      541 gatccgaaga tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttaaccggc
      601 tgtaacccag gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatggactg
      661 accaataccg tgacgcataa tcaggggaac tacgcagcga tgatggctga caccatcagt
      721 cgttacaaag agggtaagcc ggtgttttac tacacctgga cgccgtactg ggtgagtaac
      781 gaactgaaac cggggaaaga tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc
      841 gataaaaatg ccgataccaa actgccgaat ggcgcgaatt acggcttccc ggtcagtacc
      901 atgcatatcg ttgccaacaa agcctgggcc gagaaaaacc cggcagcagc gaaactgttt
      961 gccattatgc agttaccagt ggcagatatt aacgcccaga acgccattat gcatgacggc
     1021 aaagcctcag aaggcgatat tcagggccac gttgatggct ggatcaaagc ccaccagcag
     1081 cagttcgatg gctgggtgaa tgaggcgctg gcagcgcaga agtaaatatt gataaccgcg
     1141 cagataatgc tcatgtgagt aaaacaattg cagcgatggt tttaatcccg ctgaaaatcg
     1201 atgagacgtt ttctgaaggc cggggcgacg cgcagggatg cgcggcgagg gcgcgtctta
     1261 cagggatgtt acctcgcgcc cgtacccgat agccgtaaga aataagtcga atgtaccgcg
     1321 tagcggcgat tttgtgccaa aagcctgggt cgccagggtg gtggcgactg agccaccctg
     1381 gcacgttcac cagcaaaata gtgacaaagt agctataatc tggaagtgaa cgtaacattt
     1441 gccttagtgg catatttcac cttcaaatac cctctcccca ttacccgcac attttaacaa
     1501 tctatctttc gccccacatt cataaacatc cgctattatt gattcccagc ttaatcatca
     1561 cctgatgaac aaaaataatg actaaaacaa accatgagct tagcccggcg ctgatcgtgc
     1621 tgatgtctat cgccaccggt ctggcggtag ccagtaacta ttacgcccag ccattactcg
     1681 acaccatcgc gcgtaacttt tccctttccg ccagttcggc gggctttatt gttaccgccg
     1741 cgcagttggg ctatgccgca ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac
     1801 gtcgccgcct gattgtctcg atgaccttac tggcggcagg cggcatgtta attactgcca
     1861 gcagtcagtc gctggcgatg atgatcctcg gtacggcatt gaccggttta ttctcggtcg
     1921 tggcacaaat tctggttccg ctggcagcga cgctggcttc accagacaaa cgcggcaaag
     1981 tggtcggcac cattatgagc gggctgctgt tggggatctt gctggcacga accgttgccg
     2041 ggttgctggc aaacctcggc ggctggcgca ccgtcttttg ggttgcctcg gtgttaatgg
     2101 cactgatggc gctggcatta tggcgtggtc tgccacaaat gaaatcagaa acccacctca
     2161 actacccaca gttgcttggt tccgttttca gcatgtttat cagcgataaa attctgcgca
     2221 cccgcgcgtt gctgggctgc ctgacctttg ccaacttcag cattctctgg acctcaatgg
     2281 cctttttgct tgccgctcca ccttttaact acagcgatgg tgtaattggt ctgtttggac
     2341 ttgcgggagc tgccggagcg ttgggcgctc gtccggcggg cggttttgcc gacaagggca
     2401 aatcgcacca cacaacaact ttcggtctgc ttctgctgtt actttcatgg ctggcaatct
     2461 ggtttggtca cacttccgta ctggcgctga ttatcggcat cctggtgctg gacctcaccg
     2521 tgcagggcgt gcatatcact aaccagacgg taatttatcg aatacatcct gatgcgcgta
     2581 atcgcctgac cgcaggttac atgaccagct actttattgg cggtgccgcc ggttcgctaa
     2641 tttcagcctc agcctggcaa catggcggtt gggctggcgt ttgtctggct ggcgcgacga
     2701 ttgccctggt taacttactg gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt
     2761 aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga agcccatcag
     2821 tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg ttaagcgtac
     2881 actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc atggaaggat
     2941 gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg ttcggtctga
     3001 atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc atcatttatg
     3061 caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt ttgtgggttg
     3121 ctgcactgac cgtcatggca atggatgttc gccatgtttt gtatggcccg tcactgcgta
     3181 gccgtattat tcagcgtctg caaaaatcga aaaccgccct gtgggcattt ggcctgacgg
     3241 atgaggtttt tgccgccgca accgcaaaac tggtacgcaa taatcgccgc tggagcgaga
     3301 actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt acggtaatag
     3361 gggcattctc cggcagcggc ttgctgcaag gatatcccgc cgttgaagca gcattaggtt
     3421 ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc aaacaatctc
     3481 tttgcgttac cgcagcgtta gttagtgccc ttgcaggcgt aacgctattt tctattcccg
     3541 tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag gcattctggc
     3601 aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg cgcggcgaat
     3661 tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt
     3721 ggcgcgatag gcatattgct cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt
     3781 gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac gctggtcggc
     3841 ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc aacactgctt
     3901 agtgcgctgg cctacgggct cgcctggaaa gtgatggcga ttatataacc cacaagaatc
     3961 atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat cggctgaaat
     4021 taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat gctaaaattt
     4081 cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgacccg tctttgcatg
     4141 cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca ggggattaac
     4201 gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag tattcagcct
     4261 tctgaattga gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa
     4321 ctggaaaaac gcggttggat cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat
     4381 ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc gcagcataac
     4441 tgcctgcatc aactctggtc cgcgctcagt acaacagaaa aagatcagct cgagcaaatc
     4501 acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt tctcgaagcg
     4561 atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa atgactggcc
     4621 agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg agttaagaag
     4681 atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca accggtaaag
     4741 aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat aattattgcc
     4801 gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca
     4861 tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc
     4921 tgggccgata acaccgactt tgtaaaagaa ggcgatgtgc tggtcaccct cgacccgaca
     4981 gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt tcgccaaacc
     5041 caccagctga tgattaacag caaacagttg caggcgaata ttgaggtgca aaaaatcgcc
     5101 ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt
     5161 ggtcgcgaag agctgcaaca cgcccgcgac gccgttacca gtgcccaggc acaactggac
     5221 gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa actggaagat
     5281 cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cctggctggc gctggagcgt
     5341 actcgtattg tcagtccgat gaccggttat gtctcccgcc gcgcggtaca gcctggggca
     5401 caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat gtgggtggat
     5461 gccaacttta aagagacgca gattgccaat atgcgtatcg gtcagccggt cactatcacc
     5521 accgatattt acggcgatga tgtgaaatac accggtaaag tggttggtct ggatatgggc
     5581 acaggtagcg cgttctcact gcttccggcg caaaatgcga ccggtaactg gatcaaagtc
     5641 gttcagcgtc tgcctgttcg tattgaactg gaccagaaac agctcgagca atacccgctg
     5701 cgtatcggtt tatccacgct ggtgagcgtc aataccacta accgtgacgg tcaggtactg
     5761 gcaaataaag tacgttccac accggtagcg gtaagcaccg cgcgtgaaat cagcctggca
     5821 cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc cagaggtgcg
     5881 tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga cgattgcgct
     5941 gtcactggcg acattcatgc aggtgctgga ctccaccatt gctaacgtgg cgatccccac
     6001 tatcgccggg aatctgggct catcgctcag ccagggaacg tgggtaatca cttctttcgg
     6061 ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg tcggggaagt
     6121 gaaactattc ctctggtcaa ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc
     6181 cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg ccggaccgtt
     6241 gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac gctcgatcgc
     6301 gctggcgttg tggtcaatga cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg
     6361 ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc caattggcgt
     6421 ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgc gaaacccgca ccgaacggcg
     6481 gcggattgat gccgtggggc tggcactgct ggttattggt ataggcagcc tgcagattat
     6541 gctcgaccgc ggtaaagagc tggactggtt ctcatcacag gaaattatca tccttaccgt
     6601 ggtggcagtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg ataacccgat
     6661 agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct
     6721 cgcgtatatg ctctacttcg gcgcgattgt tctgctgccg cagttgttgc aggaggtcta
     6781 cggttacacg gcgacctggg caggtttggc ctctgcgccg gtagggatta ttccggtgat
     6841 cctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc tggtaacctt
     6901 cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg aaccaggtat
     6961 ggattttggc gcgtcggcct ggccgcagtt tatccagggt tttgcggtgg cctgcttctt
     7021 tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg cggcggcatc
     7081 gagcctctct aactttacgc gaacgctggc ggggtctatc ggcacatcca tcaccaccac
     7141 catgtggacc aaccgcgagt cgatgcacca cgcacagtta accgagtcgg taaacccgtt
     7201 caacccgaat gcccaggcga tgtacagtca actggaagga ctgggaatga cgcaacagca
     7261 ggcatcaggc tggattgccc agcagatcac caatcagggg ctgattattt ccgccaatga
     7321 gatcttctgg atgtctgccg ggatattcct cgtcttgctg gggctggtgt ggtttgctaa
     7381 accgccattt ggcgcaggtg gcggcggagg tggtgcgcac taagtacaac taagccagtt
     7441 catttgaact ggcttttttc aattaattgt gaagatagtt tactgactag atgtgcagtt
     7501 cctgcaactt ctctttcggc agcgccagtt cttcgttgct gttgatgcgt acgtcacgtt
     7561 ccagaatgct acgcgcaata tcctgtgctt cctgcaacga gtgcatctgg taagtgccac
     7621 actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc acgtcttcca
     7681 ttgccgcttt ccaggcatca gcgacacgct gctcatctgg cgtaccaatc agactcatat
     7741 aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca ttaccgttaa
     7801 gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct ctttctggca
     7861 tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg
     7921 gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta tgatcgactg
     7981 tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa attttctgaa
     8041 ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat catcatccct
     8101 gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttct gtcaggcgtg
     8161 tttttccagc cacaccgcaa acggttcggt atcagcggct tccatttcct gctgacggcg
     8221 ttcagacgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca acggctcttc
     8281 acgcagcaga ttacggtacg cttcagcaaa cgctttgcct gttccgccaa taccagtatc
     8341 aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga agcaggcgac
     8401 cagttcatca cacactttct gatacgcttc gccgccgtta atgctatcca gcgtttgcgc
     8461 gacgcgtttc agatcgcgga acaaatcttt acccacctgc ggtaacggga actgcgcggt
     8521 ttcgcagccg atacccagcg tcaggtccgg tttgcgacct tcgaggatca cccggttcca
     8581 gttaacgcgt gtacaggcaa gttctttact gctcatttcc ggtgcatcag ccagcgcaca
     8641 ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa
     8701 tgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta acagcgcatc
     8761 agaaggtgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcgt acagttcgtt
     8821 ttcaatctgc agcacgttgc tgttgatttg cagcctctta ccatctttct caatgccaat
     8881 cttggcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta cgtactcgta
     8941 aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc caagatcgct
     9001 caagcgaaga gaggtcgcat acggcaggta atacataccg cactcggttt tctcaaacgg
     9061 cagcgatgtt ggttttcctt gcaggaaaga agaacaaatc gccggagagg caccaaacag
     9121 ataagggatg acccaaccga aacgatagta attgcggata acgcggaaat agcccgcaga
     9181 aattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc agaatgccat
     9241 tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt
     9301 ttttagccct tcacggtaca gcgttttaaa gcgaccggtg ttagaagtgc cgtactgtgc
     9361 cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc acatccgctc
     9421 atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca gcatatgttc
     9481 aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat cggtagtaat
     9541 ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc
     9601 atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg
     9661 aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg
     9721 cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc aggcctacaa
     9781 ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat agcgtaacgc
     9841 tttaccaagg cacaaaaaaa agattactgg cccccaggag atgcgcatcc atcccgctaa
     9901 cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga ctgcaccata
     9961 gcgtttcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca atggaaagaa
    10021 acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg ctgttaaaac
    10081 taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga cttcggagtt
    10141 gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa gcgataacgc
    10201 ttcactcaca gcaagcgaac atccacggcg tccatacctg ctgcacgggc cgcctgaata
    10261 ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg
    10321 cacaacaaaa atgtgtctgg cgcaggttta tgatgtttga cgtgatcggc agcgacgacg
    10381 gcgtcaaaat aatggcgtaa ccccaggtgc gccagcaatg cctcagcgat ggcgctttca
    10441 ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac ttcgacaaga
    10501 ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt ttcacgcgct
    10561 aacgcatgcg ggtcgagatc ggcctgattc agctcaataa tcgcctgagc aatacgccag
    10621 gtgggcgagc cattaagcgc aatcatcgcc tgaacatcgt actgaagacc gtagtgccct
    10681 aatacttcgc gccacgcttt acggtgtgta ggctccgtat ccaggattgt gccatccata
    10741 tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga aaaatatgac
    10801 gctactttag cgtaattgct tgattttgtc gctgatggag aaggagaaga aaattcagga
    10861 aatggataaa gtagttattt agaaatgaga tatttttgaa ggaaattttt tggagaatat
    10921 gggcatccgg gaggatgact cactggcgtt cgcccttcag gccgttgctg gcgcaacgtt
    10981 atccacccag gtgctggtgg ttattaccac catctcttcc atactttcat tgctgaaagt
    11041 tacggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    11101 gtactctatc cagctgagct acggatgcat cgggaaaatt tatttt
//
LOCUS       DAYPHN010000154        11132 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000154 DAYPHN010000000
VERSION     DAYPHN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.154"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            146..778
                     /locus_tag="ACG0BW_003870"
     CDS             146..778
                     /locus_tag="ACG0BW_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFK7260774.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASIGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1290..2540
                     /gene="frc"
                     /locus_tag="ACG0BW_003871"
     CDS             1290..2540
                     /gene="frc"
                     /locus_tag="ACG0BW_003871"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFK7260775.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            2594..4288
                     /gene="oxc"
                     /locus_tag="ACG0BW_003872"
     CDS             2594..4288
                     /gene="oxc"
                     /locus_tag="ACG0BW_003872"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFK7260776.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNDIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAATARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            4357..5301
                     /gene="yfdV"
                     /locus_tag="ACG0BW_003873"
     CDS             4357..5301
                     /gene="yfdV"
                     /locus_tag="ACG0BW_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFK7260777.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            5375..6520
                     /gene="yfdE"
                     /locus_tag="ACG0BW_003874"
     CDS             5375..6520
                     /gene="yfdE"
                     /locus_tag="ACG0BW_003874"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFK7260778.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALDLTELVDDPR
                     FISNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(6575..10168)
                     /gene="evgS"
                     /locus_tag="ACG0BW_003875"
     CDS             complement(6575..10168)
                     /gene="evgS"
                     /locus_tag="ACG0BW_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFK7260779.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKDSVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     NIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVATVEAKAEQPMTLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLNVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINLSHQLEITPVSDDSKPEILKLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(10173..10787)
                     /gene="evgA"
                     /locus_tag="ACG0BW_003876"
     CDS             complement(10173..10787)
                     /gene="evgA"
                     /locus_tag="ACG0BW_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFK7260780.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
BASE COUNT         3075 a         2304 c         2509 g         3244 t
ORIGIN      
        1 attgtaagac taaaatactt cttgcgataa taactacaac tgtaagataa ccctttcaaa
       61 atgaccgttg ctctctgatt tctcatttca tactcaccca atatgatggc ggcgttttct
      121 aaaactgtta aagaatgagg taagtatgaa acgtttaatt atggctacga tggtcacagc
      181 aattctggca tcttcaactg tatgggcggc tgataatgca ccggtagcgg cacaacagca
      241 aactcagcaa gttcagcaaa cccagaaaac ggcagcagca gagcgtatct ctgaacaagg
      301 tttatatgcg atgcgtgacg tgcaggtagc gcgcctggct ctgtttcatg gcgatccgga
      361 aaaggcaaaa gaactgacca atgaagcttc agctttgttg tcagatgaca gtaccgaatg
      421 ggcaaagttc gctaagccag gaaaaaaaac caatgtcaat gatgaccaat atattgtcat
      481 taatgcatct atcggaattt ctgagagtta tgtagcgaca cccgaaaaag aagccgcgat
      541 aaaaattgcc aacgaaaaaa tggcgaaagg ggataaaaag ggcgcaatgg aagagcttcg
      601 cctggccggt gttggcgtaa tggaaaacca atatctgatg ccgctcaaac agactcgtaa
      661 tgcacttgct gatgcgcaga aactgcttga taaaaagcaa tactacgagg caaacctggc
      721 gcttaaaggt gcggaagatg gaatcatcgt cgatagcgaa gcactgtttg tgaactaatg
      781 ctgacaggca gtatattgag gtaaaggatt taactccctc cagccgacaa ttttttatga
      841 ccacaatacg acagattgtg gtcataaaat tatttggcgc tcgcggttct ataaactcgc
      901 attttatctt cctcaaagta aaccgcaata atcccgtgtc atcacaaatc atttattatg
      961 ttatgtttta tggtacgctt taaaaagcgt aatcataccc ttacttgtaa atcaccggcg
     1021 cttcttacag ttgtaagaat aacatcacaa atgtcgcggc tctctctgat taccgcgaac
     1081 caatatttat catattcttc ttttcgtcct cactacttac catgcagaaa ggaattggaa
     1141 gtagcgatga aatgattttc agtacttgat acaggaattt aaaaatatcg agtgcagcta
     1201 cgacgatatt ttgcggatta tttatagagg cgttatgtct taaataaacg ctaaattaac
     1261 acatatttaa ttaactaata ggtattgcta tgtcaactcc acttcaagga attaaagttc
     1321 tcgatttcac cggtgtgcaa tctggcccat cttgtactca aatgctggcc tggtttggcg
     1381 ctgacgtcat taaaattgaa cgtcccggcg ttggtgacgt aacgcgtcac cagctgcgag
     1441 atattcctga tatcgatgcg ctttacttca ccatgcttaa cagtaacaaa cgttctattg
     1501 aattaaatac caaaacagcg gaaggcaaag aggtaatgga aaagctgatc cgcgaagctg
     1561 atatcttagt cgagaacttt catcctgggg ctattgatca catgggcttc acctgggagc
     1621 atattcaaga aatcaatcca cgtctgattt ttggttcgat caaagggttt gatgagtgtt
     1681 cgccttatgt gaatgtaaaa gcctatgaaa acgttgctca ggcagcgggt ggcgcggcat
     1741 ccactacggg tttttgggac ggtccgccgc tggtaagcgc tgcagcgttg ggtgacagca
     1801 acaccggaat gcatttactg atcggtttac ttgctgcttt gctgcatcgt gaaaaaacgg
     1861 ggcgtgggca acgagtcacc atgtcaatgc aggatgccgt attgaacctt tgccgcgtga
     1921 aattacgcga ccagcagcgt ctcgataaat tgggttatct ggaagaatac ccgcagtatc
     1981 cgaatggcac atttggtgat gcagttcccc gcggaggtaa tgcgggtggt ggcggtcagc
     2041 ctggctggat cctgaaatgt aaaggctggg aaaccgatcc taacgcctat atttatttca
     2101 ctattcagga gcaaaactgg gaaaacacct gtaaagccat cggcaaacca gattggatta
     2161 ccgatccggc atacagtaca gcccatgccc gacagccaca tattttcgat atttttgctg
     2221 aaatcgaaaa atacactgtc actattgata aacatgaagc ggtggcctat ttgactcagt
     2281 ttgatattcc ttgtgcaccg gttttaagta tgaaagaaat ttcacttgat ccctctttgc
     2341 gccaaagtgg cagtgttgtt gaagtggaac aaccgttgcg tggaaaatat ctgacagttg
     2401 gttgtccaat gaaattctct gcctttacgc cggatattaa agctgcgccg ctattaggtg
     2461 aacataccgc tgctgtattg caggagctgg gttatagcga cgatgaaatt gctgcaatga
     2521 agcaaaacca cgccatctga tattaacggg ggcttctggc tcccaattta taaaaatttc
     2581 gaggttatta atcatgtcag atcaacttca aatgacagat ggtatgcata tcatcgttga
     2641 agcattaaaa cagaatgata ttgacactat ttatggtgtt gtaggtattc ctgtgacgga
     2701 tatggcacgc catgcccagg cggaaggcat tcgttatatt ggttttcgtc atgagcagtc
     2761 ggcaggctat gccgctgcgg caagcggttt tcttacccaa aaaccgggga tctgcctgac
     2821 agtttctgcg ccaggattcc tcaatggttt gaccgcattg gccaacgcaa cggtaaatgg
     2881 ttttccgatg atcatgatta gcggctccag cgaccgcgcg atcgtcgacc tacagcaagg
     2941 tgattatgaa gagctggacc aaatgaatgc ggcaaaaccg tatgccaaag cagcatttcg
     3001 cgttaatcag ccgcaggatc ttggcattgc attggcacgc gctatccggg tctcagtatc
     3061 gggtcgccct ggcggagttt atcttgattt gcctgcaaat gtcctggccg cgacgatgga
     3121 aaaagacgaa gcgttaacca cgattgtaaa agttgaaaat ccgtcgccag cattattgcc
     3181 atgcccgaag tcagtcgcta gcgcaatttc gcttttagca aaagctgaac gaccattagt
     3241 tatccttggc aaaggcgcgg cgtattcaca agcggatgaa cagcttcgtg aatttattga
     3301 aagtgcccag attccattcc tgccaatgtc tatggcgaaa gggatcctcg aagatacaca
     3361 tccactttct gcggcaactg cgcgttcgtt tgccctggca aatgctgacg ttgtcatgct
     3421 tgttggtgca cgactgaatt ggttactggc acacggtaaa aaaggatggg cggcagatac
     3481 acagtttatt caactggata ttgaaccgca ggaaattgac agcaaccgcc ccattgctgt
     3541 gccagtcgtt ggcgatattg catccagtat gcaaggtatg ctggcagaac tgaaacaaaa
     3601 cacatttacg actccactgg tatggcgcga tattttaaat atccacaagc agcaaaatgc
     3661 acaaaaaatg catgaaaaat taagtacaga tactcaacca ttaaattact ttaatgcatt
     3721 aagtgctgtg cgcgatgtat tgcgcgagaa ccaggatatt tatttagtta atgaaggtgc
     3781 aaataccctg gataatgcac gaaatattat tgatatgtat aaaccacgtc gccgtctgga
     3841 ttgtggtacc tggggtgtca tgggcatcgg tatgggctat gccatcggtg ctagcgtgac
     3901 ctctggttct ccggttgtcg ccattgaagg tgatagtgct tttggcttca gtgggatgga
     3961 gattgaaacg atttgtcgat ataacctgcc ggtgacgatc gttattttta ataatggcgg
     4021 catctacaga ggagacggtg ttgatctcag tggcgctggt gcaccatcac caacagatct
     4081 attgcaccat gcaaggtatg acaaattaat ggatgcgttt cgtggcgttg gctataacgt
     4141 cactacgaca gatgaacttc gtcatgcttt aaccaccggt attcagtcgc gcaaaccgac
     4201 cattattaat gtggtcatcg accctgcagc aggaactgaa agtggccata ttaccaaact
     4261 taacccaaaa caagtcgctg gtaattaaat actgacatcg tctcctcttg ccaactcatt
     4321 atcgatggtg aggggaccct tatcggaaat actattatgc taacattttt tattggcgat
     4381 ttattgccta ttatcgtaat catgctgttg ggttatttta gcggcagacg agaaactttt
     4441 tcagaagatc aagcccgggc atttaataaa ctggtattaa actacgctct tcctgcggcc
     4501 ctatttgttt ctattacccg ggcaaacagg gaaatgattt ttgcggacac tcgcctgacc
     4561 cttgtatcac ttgtggttat tgtcggatgt ttctttttct cctggtttgg ttgctacaaa
     4621 ttttttaaac gtactcatgc agaagcagca gtatgtgcat taattgcagg ttcacctacc
     4681 atcggattcc tgggatttgc agttctcgat cctatttatg gcgattccgt atcaacaggt
     4741 ttagtggtag caattatttc tattattgtt aacgcaatta ctattcctat tggtctgtat
     4801 ttgctgaatc cttcctcagg agcggatggt aagaagaata gtaatctgag cgcattaatt
     4861 tctgcggcaa aggagccagt agtatgggca cctgttctgg caacgatcct ggtgttggtt
     4921 ggggtaaaaa ttccggcagc atgggaccca acctttaatc tgattgcgaa ggcaaactca
     4981 ggggtggcgg tattcgctgc ggggttgact ctggctgcac ataaattcga gttcagtgcc
     5041 gaaattgctt ataacacctt cctgaagcta attctgatgc cactggcgct gcttctcgtt
     5101 ggtatggcat gtcatttgaa cagcgaacat ttgcagatga tggtactggc aggcgcctta
     5161 ccgccggcat tctccggaat cattattgcc agccggttta atgtctacac ccgcactggt
     5221 acagcgtcac tggctgtgag cgtactgggc tttgtcgtca cggctccttt gtggatttat
     5281 gtcagtcgac tggtttcata attaatcatg ttattgattt tacataaatg ccgaagtgtc
     5341 ttttcacctt cggctttttt caaggcataa gcaaatgaca aataatgaat gcaaagggcc
     5401 gtttgaaggc ttattagtta tcgatatgac acatgtcctt aatggacctt tcggaactca
     5461 acttctttgt aatatgggcg caagggtaat taaagttgag ccgccgggtc atggtgatga
     5521 tacccgcaca tttggtccct atgtggatgg acagtcactc tattacagtt ttattaatca
     5581 tggcaaagag agtgtggttc ttgatttaaa gaatgatcac gataaaagca tatttataaa
     5641 tatgcttaaa caagcggatg tattagctga gaattttcgc ccaggtacaa tggaaaaact
     5701 ggggttttca tgggaaacgc tacaagaaat caacccgcgt ctcatatatg cttcatcgtc
     5761 aggttttggg cataccggcc cgctaaaaga tgctcctgcc tacgatacca tcattcaggc
     5821 aatgagcggg ataatgatgg aaacgggata ccctgatgct ccgccagtgc gcgtgggtac
     5881 ctctcttgcg gatttatgtg gcggtgtcta tttattcagt ggaatagtga gtgcacttta
     5941 tggccgcgaa aagagccaac gaggggcgca tgtcgatata gcgatgtttg atgccacgct
     6001 gagttttctg gagcacggac tgatggcata tatcgcgact gggaagtcac cacaacgtct
     6061 gggaaatcgt cacccctaca tggcaccttt tgatgttttc gatactcagg ataagccgat
     6121 tacgatttgt tgtggtaatg acaagctttt ttctgcgtta tgtcaggcac tggatctgac
     6181 ggagctggtt gatgatcccc gatttatcag caatatttta cgcgtacaaa accaggctat
     6241 tcttaaacaa tatattgagc ggacgttaaa aacacaggca gctgaagttt ggttagccag
     6301 aatacatgaa gttggtgtac ccgtagcgcc gttattaagt gtggctgagg ccattaattt
     6361 gccacaaact caggcgagaa atatgttgat tgaagccggg ggaataatga tgccgggtaa
     6421 tccgataaaa atcagcggct gcgcggaccc gcatgttatg ccgggagcgg caacgctcga
     6481 ccagcatggg gaacaaattc gccaggaatt ctcatcgtaa attaagatgt gctggtaaat
     6541 agctcccaca tttgaacatt gtgggagccg ctatttagtc attttgttga cagaaaacag
     6601 caatctcctg gtccagctct gcaatgtgtt ctttaacaga gttcagcaac ttaagaattt
     6661 caggcttgct gtcatctgaa acgggcgtta tttctaactg atggctaaga ttaatcaact
     6721 tttgcaaatt caggatgtta gccgcaccgt ggatgcgatg aatacactga tggaaagttc
     6781 tgttatcgcc agcttctaac gcatgaaacg cagcgggtag atctttatgc gtttcatgct
     6841 ggaaagtcat gagaatctcc tgcatcagtt gtagatcgtt cgccgtatta tttttcaggg
     6901 cctcgatatc aaggtggcga tactgaggtg caatatgcgc aacctggtgt aactgactta
     6961 aatgtgtttt cagtacattc agggtcaacg gtttgaacaa acataagttc atgccgcaat
     7021 ttaacccttt ttcacgttcg ttagcctgtg cgttggctgt aagcccccag atgggtaagg
     7081 aagaattttg ctcacggagt ttgcgagtca actcaaaacc atccatattc ggcatattaa
     7141 cgtcagtaat aagcagatca taatgttgca tactgacttt gtgtagcgct tgcacaccat
     7201 cagtggcttc atcaacatca tatcctaata ggtttagctg gcgtttgagt aatagcctgt
     7261 tggtcggatg atcatccgcg attaatatgc tcaacttttc agggagtgtc atgggttgtt
     7321 ctgcttttgc ctcgacagtc gccacttgtt gggtaatttc taccgggatt gtgatcgtaa
     7381 atgtcgttcc tatgcctgga tgactttcta atgacaaatc accctgcatg tttttaatta
     7441 attctttgca gatcattaag cctaaaccag aacctgtttg ctgacgacct gcacttgttt
     7501 ggctatagcg tttaaacagt tgttgttgtt cttcctgtga taatccactt ccagaatcca
     7561 taatcgtcat tttgattaca gcgtggttgt catcaatgtt aaccagggag gtcgtaattt
     7621 tcactgcccc ctcggtggta aatttgagag cattgctcag taaattagat aagacctgct
     7681 taaacgcctg agggtcgatc ttaaccaggt aatgttcagg aaacgtactg ctgcaactta
     7741 atgcgatgga tttgcttgca gcaatcgcac cgaaagagtg acaagtgttc tggactaaag
     7801 tagggatatc gacccattgt ggttgaagtt gatagttacc cgattcaatt ttgtcgacat
     7861 caaggatttc accaattaag ccgaggagtg attgtccggt ggcgtaggca agtgaaatcg
     7921 cctccacccg ttgttccttg ctaagaccag aacccgacag aagttccagg aagcccataa
     7981 tggagcttat tggtgttctt atttcgtgac tcattgttgc cagaaactga ctttttgcga
     8041 cagttgcatt gatcgcttta tttctctcta cttcgagagc atgaattaaa tcacgcgtct
     8101 cggtaatatc ttgccaacca caaatataaa cagcatgatc gctggctggc agattgcata
     8161 atgtatgcca gtgattaatg catcttttct cgatgccatt atcaatttca aatacctgtg
     8221 tgtatattgt tcgattttct ttcgtctctg ctgtgacttc atgcgtatta gaaaaaacat
     8281 ctttaaaggg tgattcactg ttttctaatg gcaacattgc atttttgtag taatcagcag
     8341 tgaaataatg ttcaaatgca ctattgtgac taatgacatt accttgccag tttacgacat
     8401 aagttggatt cggtaaggag tccgagagtg ctttccggaa tgatatttgg ttttctaaat
     8461 caccctgaat gactttacga cgacgaactg agcgtaacag gtagaatccc cataaaaggc
     8521 tactgccaac taataaaacg gataatgtcg taacaatata aaactgctcg ctatagagat
     8581 cccatgtgtc aatggtcaca ttgggcattt taatccattt ttccgtcagg cgcagaactt
     8641 cgcttggggg aattgcattc agtgctttat taataatatc cttaagttcc ggttctccgc
     8701 gaggaaaagc gaacgaaagt gatgcattct gaacgccggg aataagaaaa tgataaagtt
     8761 cattaggata gtaatggtcg atcatgtaac gcgaatttaa ctgtgtcgcg accagagcat
     8821 caagttcacc ttccttgacc ttgtgaaatg cagcgctggc gttatcgact tttatccact
     8881 ctacctccgg atacatctct tttaattgcg aatgaagctc ataataatag ggaatggcaa
     8941 ctttcattcc tttttttaat gtttgttcac tgtcaggcgc tttttgcatg acaaaaacgt
     9001 aaggcgttgt tatgaaggct tcagcaaata aaacattatt ttctctatct tcactataaa
     9061 tagcaccagg taatatatcc catccgccgg gattaagctg tgttccggca tggatattgt
     9121 gtgaaacggt gatcggagaa aaatttaaac ctgtttgcaa ggtaataata ttaagaatat
     9181 cccccataac gccccgaacc gagccatttt catccgtcat agaataggga ggggagtaag
     9241 gattttccag cacctttaaa tcgggatgct gctttatcca ctgtttttca tgttcagtca
     9301 gttctaatgg tttgttcaga aaggccaggt ttcctgtatc aagccaattt tgtgatactt
     9361 catagcgaac ttcatttgtt aaagcatcaa caaaccgatt gagtacttca ttaagaacga
     9421 ctgaatcttt tctggttaag aaaaagttat attgacgcgg cgagttataa tatttcacta
     9481 catttaagga gtgagtgaaa tagcgggaaa tcatactgct ggtaatgatg ttactaccaa
     9541 taaagtaatc attctgtccg gctgagacgg atgctaatgc ctgatataaa tttgtaaaag
     9601 agataattgt tgcttttgga aatgattgat gaattacctc gtcaggggga taatttgcta
     9661 ctcgagcaat atttactggt tttgatgagg taagcggtcg cattgaatcg tgaagggtgg
     9721 tgacgagcgc cggaaaggta attatcagtg gtttggttgc agcaatgtca tcattaagag
     9781 gcggcgaagc aactaaatgt gataacacta tatcgacttc accttcttcc agcgcgtcca
     9841 ttgctttttg atgatcggcg tattcccgga gtgttaattt gatatttaac gctcttttta
     9901 acagatttaa ataatcagca ttaataccac gaacccgttg ctgcgaatcg gtatgcaaca
     9961 acgtagccgt ttgggacttg tgtactgcaa tcacaagatt tttcttgctc gctaaccaac
    10021 gtaattcctt gttgctcaga cgtagtggat caagtgtgac acggttgtta ctactgatgc
    10081 cacgatattc gatgtaatct tcgtctgcga aacttatggt cgaccaaaga ccacaacaga
    10141 gaagaaaaat atagggtaaa aacttcatgt ggttagccga ttttgttacg ttgtgcgaat
    10201 gtgtaaagat ccatcagtga tttacattct aatttttcca tcaggcgact tttataagtg
    10261 ctgacagttt tgttgctgat gaacattttt tcagcaatgt cattattatc cttgccatca
    10321 agaatatatc gcatgacact aatttcttgt ttcgataagg agtcgagttt ttgctggtcg
    10381 gacgttaaac ttccaacaaa ccggttgaga gagaagggga aatagcagta gccatttttt
    10441 gcagcttcaa tagccgcaat gatattgttc atgccttctt ttttactcac gaatccatta
    10501 gcgccagcat cagcacaatg tttcccgtaa aaatggtcat ttttagcgga gacgataata
    10561 ataattccgc tatattgacg cttccttagc gtttctaaca cctggatacc gttaactccg
    10621 gggatatcga catcaatgat gacgatatca ggcttaagtg tttccacccg ctgaacggcg
    10681 cttccgcctt cagtcaactc tgctaagatt tcaatatcgt ttttgatcaa taaattacga
    10741 attgctgcga tagcaagagg atggtcatca ataattattg cgttcataga ttattccctt
    10801 tgcaatgaag catctccctt ctccctgtag taatacagac ataaggcatc atctcatttt
    10861 ctcaataaaa ataagaaaat ttgagcttcc tttaagataa ttcgacaagt ataccatata
    10921 aatagtcggc ataaatgaaa tgtcaacaat aaaatcaggg attgtaccga tgatttatag
    10981 tttcacatta gcactataag tcttctcact aatcctacag gcgtaagaat cgtattgcaa
    11041 aagccacggt ttagtcctct gttgtttctt ttgcacctca tttaaattag gcctccaacg
    11101 ttcctgggat aatgtgcaac acatgcactg tg
//
LOCUS       DAYPHN010000155        11130 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000155 DAYPHN010000000
VERSION     DAYPHN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.155"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(6..740)
                     /locus_tag="ACG0BW_003877"
     CDS             complement(6..740)
                     /locus_tag="ACG0BW_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260781.1"
                     /translation="MKELKSLEIPGTGYKFFMDNAECAPNALEKIQSLINSLTEEAKE
                     LKSEATKDNTAKIQCLNLVSYCLYQVSKTLIKGNIYYDDEDTSTPEEKDSDEELYEDI
                     STSAGHAAFDAMGESSGIRSFVEQNNSTIMIEYCEQTSSKAIHVLHDVAAGVRCEAEA
                     VRKDVFGADSNTVKAFINSALLNSYSNLIVNAALCANSGEEHIVITETKHEHQQSPTI
                     EEVTALAIKQASEISDEILSLLNKTM"
     gene            complement(786..1085)
                     /locus_tag="ACG0BW_003878"
     CDS             complement(786..1085)
                     /locus_tag="ACG0BW_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260782.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIDGGCI
                     VHAGLQDDVWKEDLRRYGISEGAIANIQKVLDVGFDSVHFDCGAPVVEGLECWNW"
     gene            complement(1247..3280)
                     /gene="metG"
                     /locus_tag="ACG0BW_003879"
     CDS             complement(1247..3280)
                     /gene="metG"
                     /locus_tag="ACG0BW_003879"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFK7260783.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLAGELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVVLIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3438..4538
                     /gene="apbC"
                     /locus_tag="ACG0BW_003880"
     CDS             3438..4538
                     /gene="apbC"
                     /locus_tag="ACG0BW_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFK7260784.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(4576..4965)
                     /locus_tag="ACG0BW_003881"
     CDS             complement(4576..4965)
                     /locus_tag="ACG0BW_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFK7260785.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            5138..5722
                     /locus_tag="ACG0BW_003882"
     CDS             5138..5722
                     /locus_tag="ACG0BW_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFK7260786.1"
                     /translation="MPARIPLDPVLPKKFDSTPNEKRSKAQLDAWWDHPYGVTEPDGK
                     IVVYCLNGGAWDRPSVLGLVDNYDEACELAERQQARWIKTRSQPSFMFSIEPPFILAR
                     MPQRPDHQQEIVAEFSSRDEMNLFSLRQEEGDHVEVSPTLDHNRMNLTQLAWYSKELE
                     KSIARLENEKAATQVQHEVVLDRIREMQNDNRGF"
     gene            5723..6079
                     /locus_tag="ACG0BW_003883"
     CDS             5723..6079
                     /locus_tag="ACG0BW_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260787.1"
                     /translation="MAKIEYHRDRGNYLEIYDHESLNDINDALYEYCEKTSITDAPDA
                     FVELTVYLRDIYAIRTPPIPVIHIGYVRLYLEEDAGRYIVRHYTLDRKELPNEWNASN
                     YYNGEYGLKSANNLRS"
     gene            complement(6203..6727)
                     /locus_tag="ACG0BW_003884"
     CDS             complement(6203..6727)
                     /locus_tag="ACG0BW_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFK7260788.1"
                     /translation="MTKFGKLEAHLLTRNYRHDVHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRAHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPHEVSNLIFSHSQ"
     gene            complement(6741..7595)
                     /locus_tag="ACG0BW_003885"
     CDS             complement(6741..7595)
                     /locus_tag="ACG0BW_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260789.1"
                     /translation="MMHKSVAGEFQKEVDNTTVLLDDILSILALLEAGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSDRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWVECTRAAY
                     VRAKDKGELTRVVTNHPNNELKDHQIRELVNELRDIAVQYHGTQQLREKIARAVNNSL
                     KGGQFQPKRIRITHIGENGVEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEK
                     YGDKWDVL"
     gene            complement(7592..7813)
                     /locus_tag="ACG0BW_003886"
     CDS             complement(7592..7813)
                     /locus_tag="ACG0BW_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260790.1"
                     /translation="MNNAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRGYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            complement(7832..8080)
                     /locus_tag="ACG0BW_003887"
                     /pseudo
     CDS             complement(7832..8080)
                     /locus_tag="ACG0BW_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(8245..8430)
                     /locus_tag="ACG0BW_003888"
     CDS             complement(8245..8430)
                     /locus_tag="ACG0BW_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260791.1"
                     /translation="MRTLEVRAEDVIPGDVLITSKGQQCAVKSFWMENDKVTLFGTDG
                     SETDYDYDELLVVERAA"
     gene            complement(8427..8849)
                     /locus_tag="ACG0BW_003889"
     CDS             complement(8427..8849)
                     /locus_tag="ACG0BW_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260792.1"
                     /translation="MREKIKNPVVVLYKRETSDSYAVSITDGSQNMHDGLLMASVSPD
                     DSDYPFATFAMVGYYMAAEIEKLRVELETVKQREKDLFMENVRLKSGIAGLIHLGIRY
                     ADVEVMKIAGDAQLSTPCTDSIINSIAAGIFTKEGAAR"
     gene            complement(8849..9391)
                     /locus_tag="ACG0BW_003890"
     CDS             complement(8849..9391)
                     /locus_tag="ACG0BW_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260793.1"
                     /translation="MKVEKIDVLSFVLTDLERLDPVRVMIENYEPGKGRITIICYGKA
                     WTAAWFAMGGDDVQTFIKRVSNEYLIGYFDSQLRSTVDDDNDANLLFVKSEIIKLRRE
                     REIDAVLAREMWDEAENADDVKESCCCFGVGNKLLNLFGDDPWYADWPTVPNPKYQYL
                     ERIVNAVREGLKELDKLEAS"
     gene            complement(9388..10029)
                     /locus_tag="ACG0BW_003891"
     CDS             complement(9388..10029)
                     /locus_tag="ACG0BW_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFK7260794.1"
                     /translation="MSSISEAHMFAAGAHGGVGQKRKYTGEDYINHPVAVREIVALHD
                     GTVEMQIAALLHDVVEDTHVTIEMVRDHFGERVAEMVQALTNIARPEDGNRIQRFIIN
                     VRELEQNLDMQTRMIKLADLLDNTSSIVSRDPEFSAVYLAEKELMLDVLFHGKEIGAN
                     ADVVEYMERIGIEHPLLLSAMAKVTEGIALLKPVHIKRYEKHKSLIWSAWEAA"
     gene            complement(10149..10529)
                     /locus_tag="ACG0BW_003892"
     CDS             complement(10149..10529)
                     /locus_tag="ACG0BW_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260795.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMPNQPILDGHIQMVINSHEKGWAAIVPCTITYKLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILSGN"
     gene            complement(10529..11128)
                     /locus_tag="ACG0BW_003893"
     CDS             complement(10529..11128)
                     /locus_tag="ACG0BW_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260796.1"
                     /translation="MDKGIVKFEGDFDESAKTFIDFVAKRWSEQWKNLEKRANEFDRF
                     MDAMDTAKEALDAGTPLDLESLFNGEVASAMFATMFAGEFVRSGAKNYLELDYNVPAI
                     GDFVVTIQRKEGKTPGERVAELEAVVDQRNGECDRLINELHALREERICAGSNTRNAA
                     DIYFQLVEECQIPPGGSLVDYVRHLMAEVNSSHKDGEVR"
BASE COUNT         2839 a         2787 c         2474 g         3030 t
ORIGIN      
        1 atgttttaca tagttttgtt tagcagagag agaatttcat cactaatttc actcgcctgt
       61 ttgatagcca atgcagttac ctcttctatg gttggtgact gttgatgctc atgtttggtt
      121 tcagttatga caatgtgttc ttcacctgaa tttgcgcata gtgccgcatt gacgataagg
      181 tttgagtaag aatttagcag agcactgttg atgaacgctt tcacggtatt actgtctgca
      241 ccaaatacat cttttctgac cgcttcagcc tcacagcgaa cccctgcagc aacatcgtga
      301 agaacatgta tggccttgga gcttgtttgt tcacagtact caatcattat tgtcgaattg
      361 ttctgttcaa caaacgatct gatgcctgac gattcaccca tagcatcgaa ggcagcatga
      421 cccgcactgg tagagatatc ttcatagagt tcttcatcgc tatctttttc ttcaggtgtc
      481 gaggtgtctt catcgtcgta atagatattg cctttaatca gtgtcttgga cacttgatat
      541 aaacaataag aaacgagatt taagcattgg attttagctg tattgtcctt tgtcgcctcg
      601 ctcttcagtt ctttcgcttc ttcagtaagg ctgttgataa gactttgtat tttttccaac
      661 gcgttaggcg cacactccgc gttgtccata aagaatttat aaccagtccc agggatttct
      721 aaacttttca actctttcat ttctttgata atcctaaaaa caaactacta atgctttaat
      781 atctgttacc agttccagca ctcaagccct tcaacgaccg gcgcaccaca atcaaaatgt
      841 acagagtcaa agcctacatc aagtactttc tgaatgttag caatcgcgcc ttcggatata
      901 ccatatcgac gcaagtcctc tttccagaca tcatcctgta acccggcgtg aacaatacaa
      961 ccgccatcaa tttgtgaaac ccaatccagc tcattgcttg cgatcatgaa tcggcgagca
     1021 tcttcctctg atgtatgaga tgaactgatc aatgccacct ggtaggcttg ggtaatgttc
     1081 aacatttcaa ctactccaaa acaagttgtt ttcttatgtg gcttattatc tcaatcaaca
     1141 aaaggtagaa aacaaattgt tttagggctt gtgtaaatca ggagggattg tgtctatatg
     1201 aaaatgccca gcgtgttgcc gggcattgtg aaaaatggtt gtttagttac ttaacttggt
     1261 ggcctggttt agcaccagca tccgggctta acaggaagat atcttttccg ccagggccag
     1321 cagccatcac catgccttcg gagataccga agcgcatttt acgcggtgcc aggttagcca
     1381 ccataatggt gtgacgacca atcagtgcct gtggatctgg ataagcggaa cgaatgcctg
     1441 agaagacatt acgtttttct ccgccgagat ccagtgtcag gcgcagcagt ttgtcagaac
     1501 cttcgacaaa ctccgcgttt tcaatcagta ccacgcgcag gtcaactttg gcgaagtcgt
     1561 caaaggtgat ggtttcctga atcggatcgt ctgccagcgg gccagttacc ggtgcggcag
     1621 cggctttgac ttcttcttta gacgcttcca ccagtgcttc aacctgcttc atatcgatac
     1681 ggttatacag agctttgaac ggattcactt tatggcccaa cagcggttgc tggataccat
     1741 cccaggtcag ttccgtattg aggaatgcct ctgcacgttc agtcagtttt ggcagtaccg
     1801 gtttcaggta cgtcatcagc acgcggaaca ggttaatgcc catagagcaa atcgcctgca
     1861 gatcggcatc gcgaccttcc tgcttcgcca ccacccacgg agcctgttca tcgacatagc
     1921 ggttagccag gtcagccagt gccatgattt cacggatagc tttaccaaat tcgcggcttt
     1981 cccatgcttc gccaatcact tcagcggcat cagtaaaggt tttgtacagt tgcgggtcag
     2041 ccagttcgcc tgccagcaca ccgtcaaaac gcttattgat aaagcccgcg ttacgggacg
     2101 ccagattcac cactttgttc acgatatcgg cattcacgcg ctgaacgaaa tcctccaggt
     2161 tgagatcgat atcatcaatg cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt
     2221 cagcgtcaaa atgattcagc caggtgctgg ctttaataaa ggtgccgcga gacttggaca
     2281 tcttcgcgcc gttcaccgtc acatagccgt gaacaaacag gttggtcggc ttgcggaagt
     2341 tgctgccttc cagcatggca ggccagaaca ggctgtggaa gtaaacaata tctttaccga
     2401 tgaagtggta cagctcggcg gtggagtctt tcttccagta ttcatcgaag ctggtggtat
     2461 caccgcgctt gtcgcacaga ttcttgaagg aacccatgta gccaatcggc gcgtccagcc
     2521 agacgtagaa gtatttgccc ggtgcgttcg gaatttcaaa accgaagtaa ggtgcgtcac
     2581 gggagatatc ccactgttgc aggccagatt caaaccactc ctgcattttg tttgccacct
     2641 gctcctgcaa cgcaccgctg cgggtccatg cctgcaacat ttcgctgaaa gaaggcagat
     2701 caaagaagaa gtgttcggaa tcacgcatta ccggcgtagc gccagaaact accgatttcg
     2761 gctcgatcag ttccgtcggg ctgtatgtcg cgccgcagac ttcacagtta tcgccgtatt
     2821 gatctggcgc tttacatttc gggcaggtgc cttttacaaa acgatccggc aggaacatgc
     2881 ctttttccgg gtcgtacaac tgagagatag tgcggttttt aataaaaccg ttctctttca
     2941 ggcgagtata gataagctca gacaactgac ggttctcttc gctgtgcgtc gagtgatagt
     3001 tgtcatagct gatgttaaag cctgcgaaat cagtctggtg ttcctgactc atttcgccaa
     3061 tcatctgctc cggtgtgata ccaagctgct gtgctttcag catgatcggc gtaccgtggg
     3121 catcgtcggc acagatgaaa ttaacctcgt ggccgcgcat tcgctggtaa cgaacccaga
     3181 catcagcctg gatgtgctcc agcatatggc cgagatggat tgagccgtta gcgtacggca
     3241 gcgcgcacgt caccagaatt ttcttcgcga cttgggtcat agtaggcatt acttctttgt
     3301 agtgaaaagg ggcttgatag taacaaaatg gccttatgtc tgccatgtga taacagcatt
     3361 tctcataaat gattaaatgt cgtagctgga gtacactaca aggcgacaat tgcgcaaatt
     3421 aaaataaagg agacgggatg agcgagtcca aatcgccgga tgccctgaga gcaatggtag
     3481 ccggtacgct ggctaatttt cagcacccaa ccctgaaaca caacctgact acgcttaaag
     3541 cgttacacca tgttgcgttg atggatgaca cactgcatgt cgaactaatc atgcctttcg
     3601 tgtggaataa gccttttgaa gacctgaaag agcaatgtag tggtgatctg ctccgtatca
     3661 ctggcgcaaa ggctattgac tggaagctgt cgtacaacat tgccacgctt aagcgcgtca
     3721 aaaaccaacc aggcattaat ggcgttaaga acattatcgc cgtcagctca ggcaagggcg
     3781 gcgtgggtaa atcctccacg gcggtaaacc tggcactggc gttggctgct gaaggtgcga
     3841 aagttggtat tttggatgcc gatatctatg gtccatcaat tccaaccatg ctgggcgcgg
     3901 aaaaccaacg tccaacctca cctgacggta ctcacatggc acctatcatg tctcatggcc
     3961 tggcaaccaa ctctattggt tatctggtca ccgacgacaa tgcaatggtg tggcgtggac
     4021 cgatggccag caaggcgctg atgcagatgt tgcaggaaac tctatggcct gatctggact
     4081 atcttgttct cgatatgccg ccgggcactg gtgatattca gttgacgctg gcgcagaaca
     4141 ttcctgtaac tggtgctgtt gtggtaacta cgccgcaaga catcgcgctg atcgatgcga
     4201 agaaaggcat tgtgatgttc gaaaaagtcg aagtgccggt actgggtatc gtcgaaaaca
     4261 tgagtgtgca tatttgcagt aactgcggtc atcacgagcc aatttttggc accggtggcg
     4321 cacagaaact ggccgagaaa tacaacacgc agttgttagg ccaaatgcca cttcatatct
     4381 ctcttcgtga agatctggat aacggaacac caaccgttat tagtcgtcca gatagcgaat
     4441 ttacggctat ttatcgtgac ctcgcagatc gcgttgctgc ccaaatgtat tggcaaggtg
     4501 aagtaatacc tggcgaaatc gcattccgcg cagtgtgata aaaaggcggt gcattgcacc
     4561 gcctaatcag ttctcctaca tcacgggaca atcatcaaac tcgccacttc ttgcgtcgtt
     4621 gataatgtga gtgatcacac caaacacggc gttgctgccc gtgcatccat cgtcatcctt
     4681 tggcaatgct tccttcttac cggttcttaa atcctccaga tgctgacgtg ggtacttccg
     4741 gtatcttttt acgcgatatt ccccatccag agcgcataca agcagtgaac catcaaccgg
     4801 ggtaagtgag gaatcgacca ccagcaatgc accctgtaat attccctcac gatgatggct
     4861 atcagctgcc ctcatgaagt aggtcgctga aggatgtcta attatctgct gatcaagaga
     4921 aatacggctc tcagcataat ccgccgcagg agaagggaag cccatagcgt tttcacctca
     4981 aaatactgtt tatccataca gtatacatta aagatgcacc tttggtgcaa acgcgttatg
     5041 ggtcttaacc cctctgatga ctttgtgcgc tttgctacta ttcatcacca acggatcagc
     5101 gtaacctcgt tgccaatcag ttaataagga attagctatg cctgcccgca ttcctctcga
     5161 tcctgtattg cccaaaaaat ttgacagcac tcctaacgag aaacgctcca aagcccagtt
     5221 ggacgcctgg tgggatcatc cctatggagt tacagaacct gacgggaaaa ttgttgttta
     5281 ttgtctgaat ggtggcgcgt gggaccgccc atccgtgctt ggtttggtag ataactatga
     5341 tgaagcctgt gaacttgccg aaagacagca ggcaagatgg ataaaaaccc ggtcacagcc
     5401 gtcattcatg ttttcaatag aaccgccatt tatactggca aggatgccgc agcgaccgga
     5461 tcatcaacaa gaaattgttg ctgaattttc ctcaagggat gagatgaatc tcttctcatt
     5521 aaggcaggaa gaaggggatc acgtcgaggt gtcgccaact ctcgatcaca accggatgaa
     5581 ccttacccaa cttgcctggt acagcaaaga attagagaag tctattgccc ggcttgaaaa
     5641 cgaaaaagcc gctacccaag tccaacatga agttgttctg gaccggatta gagaaatgca
     5701 aaacgataat aggggatttt gaatggctaa aatcgagtac catcgcgatc ggggtaatta
     5761 cctggaaata tacgatcatg aatcccttaa cgatatcaac gatgcgttat atgaatactg
     5821 tgaaaaaacg agcatcacag atgcacctga tgcatttgtc gagctaacgg tatatctccg
     5881 cgacatctat gcaatacgaa cgccgcccat accggtaatt cacattggct atgtccgcct
     5941 gtacctcgaa gaagatgcag gtcgttacat cgtgcgccac tatacattgg acagaaaaga
     6001 gcttcccaat gaatggaacg cgagtaatta ctataacggt gaatatggct taaaatccgc
     6061 taataattta cgctcataat ctatacagca aggtataaca acaagcctat gagctgccaa
     6121 caacgtgatt ttctcaaata agaaaacgta gaaaacaaat tgtttaaggc cactagttgt
     6181 ggccttaatg gagaagtgat acttattggc tatggctaaa aatcaaatta gacacttcat
     6241 gtggttgtag ctcgtcaaga tccggtgcca caaaaccttt tccgaaagac ttcacaagtg
     6301 attcgccagc cgcatcattg tcacctatta cacgaaagtc atacggcagc attgccagtt
     6361 gacgacgtaa tcctgctgag aaagtcgaac ctaacagcgc ccacgaattt ccgcccgcgt
     6421 tgtgcactgc tgcactttta aaaatcgact cagttatcca tatcggtcca tcttttaaag
     6481 gaagataggt tccccatagt agctgtctgc cgtgcgctcg tgtgaaatac cgggccatct
     6541 tcggattgct atgctgctta ggagcgcctg gtctgtatgt ctgatacccg gccaacatcc
     6601 catcaaacct gtatagaggg aacgtaacaa ctccttcgct ttcatcgatc cactgtctat
     6661 atggatgcac atcatggcgg tagttacgag ttagcaaatg agcttccagc tttccaaatt
     6721 tggtcataac ttcgcatccc tcataacacg tcccatttat caccatattt ctccatagct
     6781 cttagccagt aaacatggcc actaggcata gtgatgtgag gctcgccaaa ctcttctcca
     6841 tgcacatcaa agatgtcccc aacagagaat tgggggattt ctacgccgtt ttcacctata
     6901 tgcgtaattc gtatacgttt cggttgaaat tgtccacctt tgagcgagtt gttcactgct
     6961 ctggcaatct tctcccgcaa ttgctgcgtt ccgtggtact gaaccgcaat atctctcaac
     7021 tcgttcacca gttctcgaat ctggtgatct ttaagctcat tatttgggtg attggtaaca
     7081 actctggtta attcaccctt gtcttttgct cttacgtatg cagctcttgt gcattcgacc
     7141 catgtattac cgcctacatt aacttcgtag attaggctgg acttatctgt caattcggaa
     7201 caaatacggg ccgtggtctg gcaactagag cattcgcagt tatcgcggaa accgtggtct
     7261 actggcttcg gtttcacatc atcaactttt tctaccctat cggaatttaa atacttcgaa
     7321 gccttgctat gccaatcgct ggttttagga tcttgtccat ccatcaaacg ccaggcttca
     7381 gcgattaact cataactagt gactgtagct tcctgcgcat caccaataag tcgtgtaatc
     7441 tctctttcaa gccgaccgcc taactccgtt ttagtgcaat gttctgacca atcgccagcc
     7501 tcaagaagcg cgagaatgct taaaatatcg tccaatagaa cggtagtatt atcgacttcc
     7561 ttctgaaact caccggctac tgatttatgc atcattcgtc atcctcatcc gagtcatcct
     7621 catcacagga tgagagtagt gggttagtca ttcgccctac ttgagtggcg tagccacgcc
     7681 gacagagatt gcgcagcaca ctgtatattt cgaacatctc ggttcgttca tcgccaacat
     7741 caagctcaga tgctatagcg tggcattcag tcgcgagagc cgatattttc tgaaataact
     7801 ctgcattatt cactgttcga ctcctgcggc gttctggtag tggcatccag tgtgtgacat
     7861 tgctaatcag accatattca ttagtttgag gatggttgcc gttatcgtct ccgtatttaa
     7921 gactctccat aaagccataa tgcctatcac cattaacgct cacaaagccg taataagcag
     7981 gtataacgcc gatctcacac ataaccagta aaggaaaact agttctccaa tttagctcgc
     8041 caattacagg cattcgctca ctacagctta tccaaccatc cttagatgcg gctgtattgg
     8101 cataggcctt acggtgcttc tgtagctctg ctgcaatcgc tctcacgact tcaactggtg
     8161 cctttgcagc aaactcaatg ttggtgatca gctcattaag atatttctcg cttgggtagc
     8221 atttcttatt gttaacggtg gtcattaggc agctctctca acaacaagca attcgtcgta
     8281 gtcataatca gtttcggaac catccgtacc gaacagagtc actttgtcat tttccatcca
     8341 aaaagattta accgcacatt gttgaccttt agatgttatg agcacatcac cagggattac
     8401 gtcttcagcg cgaacctcta gtgttctcat cgtgctgccc cctctttggt gaaaatgcct
     8461 gctgcaatgc tgtttatgat gctgtcagtg catggagtag aaagctgggc atctccagca
     8521 attttcatga cctcaacatc cgcatatcga ataccgaggt gtatcagacc ggctatacct
     8581 gacttaagtc gaacattttc cataaacaga tccttttccc gctgtttaac ggtctccagc
     8641 tcaacacgca acttctcaat ttcggcagcc atgtagtaac caaccatagc gaaagtggcg
     8701 aaagggtagt cagaatcatc aggagatacg gaggccatta gcagaccatc gtgcatgttc
     8761 tggcttccat cagtgatgga taccgcataa gaatcgctgg tttcccgttt atacaatacg
     8821 acgaccgggt tcttgatttt ctctctcatt acgatgcctc taacttatct aactctttca
     8881 gcccttcacg taccgcgttc acaatgcgtt ccagatactg gtatttcggg tttggtaccg
     8941 ttggccagtc ggcataccac ggatcatcac caaagagatt cagcagtttg ttaccgacgc
     9001 cgaaacaaca gcagctttct tttacgtcat cggcgttttc cgcctcgtcc cacatttcgc
     9061 gagccagtac ggcgtctatt tctctctctc ttcgtaactt tatgatttct gacttcacga
     9121 aaagcagatt tgcatcgttg tcatcgtcga ccgtgcttcg cagttgagag tcgaaatagc
     9181 cgattagata ctcgttgctg acccgcttaa tgaacgtctg cacatcatca ccgcccatag
     9241 caaaccaagc cgcagtccac gcttttccgt agcagatgat ggtgattctt cccttaccag
     9301 gttcgtagtt ttcaatcatt actcgaaccg gatcgaggcg ttccagatcc gtcagcacaa
     9361 aggaaagaac atctattttt tcgaccttca tgcagcctcc cacgcgctcc agatcagcga
     9421 tttgtgtttt tcataacgtt tgatgtgtac cggtttcaga agcgcaatac cttcagtgac
     9481 tttcgccatt gcgcttaata gcaacgggtg ttctatacct atcctctcca tgtactcgac
     9541 aacatcagca ttggcaccaa tctctttgcc gtgaaagagt acgtccagca tcagttcttt
     9601 ctctgcgagg tagacagctg aaaactcggg atcgcgactc acgatagaag aggtattgtc
     9661 gagcaggtca gccagcttga tcatgcgcgt ctgcatgtcg agattttgct ccagctcccg
     9721 gacattgatg atgaaacgct gtattcgatt gccgtcttct ggccttgcaa tgttggtcag
     9781 agcctgaacc atttcagcta cgcgctcacc gaagtgatca cgaaccatct ctatggttac
     9841 atgggtgtct tcgaccacat catgcagaag cgccgcgatt tgcatctcca cggttccgtc
     9901 atgcaatgca acaatctctc ggacagcaac cgggtggttg atgtagtctt cgccggtata
     9961 ttttcttttc tgaccaacgc caccatgagc gccagcggcg aacatatgag cttcggaaat
    10021 actagacatt cttaactccg ttgttttcta gttgtggtta ttttcacaaa taagaaaagg
    10081 tagaaaacaa tttatttaag gccacaactt atggccttta tttattcatg ccgaaggatt
    10141 tgtagcgtct aattgccgga taaaatgggg tgaggtgctt gccagtaggc tccaccggtc
    10201 catgtgaacc ccatgcgaat aagagtgttt attgcggctt tggtagactc cgattcgaca
    10261 ctggcttttc gaaactcttg aacctctttc gccaacttgt atgtaattgt gcaggggacg
    10321 attgcagccc agcctttctc atgactgtta atgaccatct ggatgtgacc atccagaata
    10381 ggttgattag gcatgttatt gaaaatgtct gcctgttttt gaatgtgaag aaaaagcctt
    10441 gccagctcca tttgctcact acgagacaag ggattatcaa gagggctttt tgcaaaagcg
    10501 gctatacgct gtgcgtcaat gccaaacatc agcgcacctc accatcttta tggctggagt
    10561 taacttccgc cattaaatgt ctgacgtaat cgacaagaga gccacctggt ggaatctggc
    10621 attcctcaac taactggaag tagatatccg ctgcattacg tgtattgcta cccgcacata
    10681 ttctttcttc ccgaagtgca tgaagctcgt tgatcaaacg gtcacactct ccgttacgct
    10741 gatccacaac ggcctcaagc tctgcgacgc gttcacctgg cgtcttacct tctttgcgtt
    10801 ggatggtaac gacgaaatcg ccaattgcag ggacgttgta atccagctca aggtagtttt
    10861 tggcaccgct gcggacgaac tcacccgcga acatggtggc aaacatcgca gaggccactt
    10921 cgccgttgaa aagtgactcc agatctaacg gagtcccggc atcaagagcc tcttttgctg
    10981 tatccattgc atccatgaac cgatcaaact cattggcgcg cttttccagg tttttccatt
    11041 gctcgctcca acgtttggca acgaagtcta tgaatgtttt cgcggattca tcaaaatccc
    11101 cctcaaactt aacgatgcct ttatcaataa
//
LOCUS       DAYPHN010000156        10885 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000156 DAYPHN010000000
VERSION     DAYPHN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.156"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            193..1008
                     /gene="yahL"
                     /locus_tag="ACG0BW_003894"
     CDS             193..1008
                     /gene="yahL"
                     /locus_tag="ACG0BW_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFK7260797.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1421..1666
                     /gene="yahM"
                     /locus_tag="ACG0BW_003895"
     CDS             1421..1666
                     /gene="yahM"
                     /locus_tag="ACG0BW_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFK7260798.1"
                     /translation="MAVQLFITLLNQIPLLSSLQSGTLPLFGYSGRVRAMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(1683..2354)
                     /locus_tag="ACG0BW_003896"
                     /pseudo
     CDS             complement(1683..2354)
                     /locus_tag="ACG0BW_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
     gene            2501..2776
                     /gene="yahO"
                     /locus_tag="ACG0BW_003897"
     CDS             2501..2776
                     /gene="yahO"
                     /locus_tag="ACG0BW_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFK7260799.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2877..4463)
                     /gene="prpR"
                     /locus_tag="ACG0BW_003898"
     CDS             complement(2877..4463)
                     /gene="prpR"
                     /locus_tag="ACG0BW_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFK7260800.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALAAPFSSALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            4702..5592
                     /gene="prpB"
                     /locus_tag="ACG0BW_003899"
     CDS             4702..5592
                     /gene="prpB"
                     /locus_tag="ACG0BW_003899"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFK7260801.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERARAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQAK"
     gene            complement(5538..6077)
                     /locus_tag="ACG0BW_003900"
     CDS             complement(5538..6077)
                     /locus_tag="ACG0BW_003900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260802.1"
                     /translation="MRRYASYPTYSSVHPAGLISRVSVASGIGCRMRRHASYPTYSSV
                     HPVGLISRVSGASGIGCRMRRHTSYPTYSSARPVGLISRVSGASGIGCRMRRYASYPT
                     YSSVHPVGLISRVSGASGIGCRMRRYASYPTYSSVHPVGLISRVSGASGIGCRIQPTG
                     VFYFAWLRANRSSSFSSYW"
     gene            6125..7294
                     /gene="prpC"
                     /locus_tag="ACG0BW_003901"
     CDS             6125..7294
                     /gene="prpC"
                     /locus_tag="ACG0BW_003901"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFK7260803.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDIIDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            7328..8779
                     /gene="prpD"
                     /locus_tag="ACG0BW_003902"
     CDS             7328..8779
                     /gene="prpD"
                     /locus_tag="ACG0BW_003902"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFK7260804.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8819..10705
                     /gene="prpE"
                     /locus_tag="ACG0BW_003903"
     CDS             8819..10705
                     /gene="prpE"
                     /locus_tag="ACG0BW_003903"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFK7260805.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEECTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2677 a         2776 c         2914 g         2518 t
ORIGIN      
        1 taacttttta gttaaggaga gcgaaataat atcaaagtag cagtaaaacc tataacgtaa
       61 attcaaattg ttaaattaac tccctccagt acacaatact tcacacgtta gttatgagcg
      121 atttctgata gtgcctggtt taatcagggc tttattatct gcgacgttta tttttattta
      181 agagagatcg cgatgatatc attaaaagct ccgcacaata atttgatgcc atatacgcaa
      241 caaagcatac ttaatacagt aaaaaacaat cagttaccag aggatatcaa aagctccctg
      301 gtctcctgtg tcgatatatt caaggttttg attaaacaat attatgatta tccttatgat
      361 tgtcgtgatg atttagtcga tgacgacaaa ctcattcatc tcatggctgc tgtacgagac
      421 tgtgagtggt cagacgataa cgcactcact ataaatgtgc agtttaatga tttccctgga
      481 ttttatgact ggatggatta ccctgatcac ccggttaagt ttgtttttcg catacttgaa
      541 aatcaaaaag ggacagtttg ggtatatgac caggatgatg cgtttcttga tattaaagca
      601 aatgtgcagg ctggacgctt taccgggctt aagaaattag tgcagtttat cgattcagtg
      661 cgaacagatt gcaaatgtat tcttcttgaa caccatatgc ctttgctgag acttttccct
      721 aaagggaaag agtgtatgca tgtggaaaaa tggcttcgcg aaatgtcgtc tattccggag
      781 accgacgcac ccattaagca ggcgcttgcg catggattat tattacactt aaaaaatatc
      841 taccctgttt ttcctgaatc actggtgatg ttattgctct ctgttctgga cgtaaaaaca
      901 tacagagatg atgcgcgatt aaatcaatgg atatctaata gagtgcagga attaggtgac
      961 agatattatc ctgtaaataa gcacgttaaa atccgctaca ctctttagtt cagaaagggt
     1021 tccacctgcg agttgttatt ccaccagaga gtgaatcgca aagcaggtga tcatttcatc
     1081 tgtatatccg tatgccaaat ggcattccac cgcctttttc gccgatattt tgttgtgatc
     1141 ggcgacactt cgcttaaaaa agcatcagca gtggtttcgc agctatacgg tgtataaaaa
     1201 atgatctcat gcagatgttt tgtgaatgtg tgggttgaca ttcatatgaa aaaaatcata
     1261 attccatcat gtttgtgtgg ggtcttttct gtatcttacg catcgcactc aagcctgaca
     1321 gaaaatatgc tgtaaggctc atatcaaaaa ccgccattag ctcatcagga agagcagacg
     1381 acaaccagga ttgttgtatg gtacggggtt cgaggcctcg atggcggtcc aacttttcat
     1441 aactcttctg aatcagatcc ccttattatc atccctgcaa agtggaacat tgccattgtt
     1501 tggctattca ggacgggtca gagcaatgaa aaaagcccac acaggggaga gtgggctgaa
     1561 atgggaagct aaagactcaa gtaaacttat cggaaataag gaccacgcat tacgggggct
     1621 atcatcgccg atggtcggga ttcggcaaat caggttgatt acgtagagag taaattatct
     1681 gctcaccgct gtgtcacccc ttcgtaaatc aggcgtagcg cgaatacacc aataattgca
     1741 ccaataaccc gactggcaac gcgttgcata cgcccataag cacgacgcac agcgggcaaa
     1801 gaaaacgcct gactaagaaa aacgcgccag ataattgatg cgagcacaat ccccgctcag
     1861 gccattaaac gtgcccatgt tggtgtttcg gcatttaatg ttactgagaa aatactgata
     1921 aaaaataaaa cggtttgcgg gttagagaga tcggtaatta atccacggcg aaaaaagaca
     1981 taccaggggg cgttaatcgg ttgttgtagt gtgctcattt gcggtgttga ctggcggcgc
     2041 atgctgcacc acgcaaacca taagagataa gcgccgccga cgattctgat aagcgaaaaa
     2101 atctcctcac actgcgtaat tagcgttgca agaccaaaca aacccaaccc ggaataaaat
     2161 gcatcgccca gcgccacgcc cagcccggtc agcacccctg cgcgtcgacc ggaagccagg
     2221 ctggtttgta ctaccacaaa gagattggct cccggattaa aaaaagtaat cacgaacagt
     2281 cctacggtca ggtaaacggc gtgcaaagga tccatagtga tttcatccat aaataagtga
     2341 actaactgca tcatatttct accaaaaata atcgggtgcg agagagatca caaagtgtct
     2401 tatttccggt tactggcgtt tatgccctga ctgaactaat tattaatcaa cccaataatg
     2461 tgggtgggtg atagtgtgat aacaactctg gagccgtaat atgaaaataa tctctaaaat
     2521 gttagtcggt gcgttagcgt ttgccgttac caatgtctat gccgctgaat tgatgaccaa
     2581 agcggaattt gaaaaagttg aatcgcaata tgaaaaaata ggtgatattt caaccagtaa
     2641 tgaaatgtcg actgcagatg caaaagaaga tttaatcaaa aaagccgatg aaaaaggggc
     2701 tgatgtgttg gtgctgacct ctggtcaaac tgacaataag attcacggca cggcaaatat
     2761 ttataagaag aagtaattct gaaacctatg taaacatccc cgatgcgtaa gtttatcggt
     2821 gatcatcatc tattgaaatt tatgccggat aaagcgttcg cgctgcattc ggcagttcag
     2881 tttttcagcc gccgccagaa cgtcgtccgg ctgataccta aataattcgc cgctgctgtt
     2941 ttatcgcctt taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg agcgggaatt
     3001 ttcgctgatt cgcgcgctag ttccggcagt agcagctgca aaaactgcgg cgttaaatcc
     3061 ggcgtcggtt ccacacttaa aaacagcgcc agtcgctcca tcatattgcg cagttctcga
     3121 atattgcccg gccagtcgta gtgcaccagc acggtttcgc ttgcctgtaa tccctggcgt
     3181 aatgcggaag aaaacggggc agcaagtgca gccagagaca ctttcaaaaa gctttccgcc
     3241 agcggcagaa tatctgccac ccgctcacgc agtggtggca gttgcaggcg taaaatgctc
     3301 agccgataaa acaggtcacg gcgaaactgc ccttgccgca tatcttcttc cagattgcag
     3361 tgagtggcgc taatgactcg cacatccacc ggaacaggct gatgcccgcc gacgcgggtg
     3421 acctcttttt cttccagcac ccgtaacagg cgagtctgca aaggtagcgg catttcgcca
     3481 atctcatcca gaaacagcgt accgccgtgg gcaatttcga acagcccggc gcgacctccg
     3541 cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gctccgcttc cagcagcgat
     3601 tcggcaatcg ctccgcagtt gacggcaaca aacggatgcg actttttgcc ctgtcgcgca
     3661 tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt gcccgtcccc
     3721 gtttcccctt caatcaacac cgctgcactg gagcgggcat acagcaaaat agtctgccgt
     3781 acctgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg agtacgcagg
     3841 gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat atccagcgca
     3901 tcgctgaatg cctggcgcac ggtggcggcg gaatagataa aaattccggt cattccggct
     3961 tcttctgcca gatcggtaat tagccccgcg ccgaccaccg cttcggtgcc gttagctttc
     4021 agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt agctacgttg gtcgaggcgc
     4081 aaattaaagg ttttttgaaa cgccaccagt gccggaatag tttcctgata agtgacaacg
     4141 ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc gtagccgctc
     4201 ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc gttagagcca
     4261 gcggcgatga tggcgtcgca gcgttcgttt gccagtttct tgcggatgta ggtcactgct
     4321 ttttcaaagc caagctgaat aggggtaatg ttcgccagat gatcaaactc gaggctgata
     4381 tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg tttgtcgtca
     4441 ttcagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa tattgggttt
     4501 agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgactgaa acgttaactg
     4561 aaacgcatat ttgcggatta cttcatgact ttatttataa caaattgaaa ttaaacattt
     4621 aattttattc gatcaattgt ggcacacccc ttgctttgtc tttatcaacg caaataacaa
     4681 gttgataaca aaggatgggc tatgtctcta cactctccag gtaaagcgtt tcgcgctgca
     4741 ctgtctaaag aaaatccatt gcagattgtt ggcaccatca acgctaacca tgcgctgctg
     4801 gcgcagcgtg ccggatatca ggcgatttat ctttccggcg gtggcgtggc ggcagggtcg
     4861 ctggggctgc ccgatctcgg tatttctacc cttgatgacg tgctgaccga cattcgccgt
     4921 atcaccgacg tctgctcgct gccgctgctg gtagatgcgg atatcggttt tggttcttct
     4981 gcttttaacg ttgcgcgcac cgtgaaatcg atgattaaag ccggtgcggc gggattgcat
     5041 attgaagatc aggttggtgc gaaacgctgc ggccatcgcc cgaataaagc gatcgtctcg
     5101 aaagaggaga tggtggatcg gatccgcgcg gcggtggatg cgaaaaccga tcctgatttt
     5161 gtgatcatgg cgcgcaccga tgcgctggcg gttgaagggc tggatgcggc gatcgagcgt
     5221 gcgcgggcct atgttgaagc gggtgccgag atgttgttcc cggaggcgat taccgaactc
     5281 gctatgtacc gccagtttgc cgatgcggtg caggtgccaa tcctcgccaa cattaccgaa
     5341 tttggcgcaa caccgctgtt tactaccgat gaattacgca gtgcccatgt tgcaatggcg
     5401 ctgtacccgc tttcagcgtt tcgcgccatg aaccgcgccg ccgaacatgt ctacaacgtc
     5461 ctgcgtcagg aaggcacgca gaaaagcgtc atcgacacca tgcagacccg caacgaactg
     5521 tacgaaagca tcaactatta ccagtacgaa gagaagctcg acgacctgtt tgcccgtagc
     5581 caggcgaaat aaaaaacgcc cgttggttgt attcgacaac cgatgcctga tgcgccgctg
     5641 acgcggctta tcaggcctac gggatgaact gaactgtagg tcggataaga cgcatagcgt
     5701 cgcatccgac aaccgatgcc tgatgcgccg ctgacgcggc ttatcaggcc tacggggtga
     5761 actgaactgt aggtcggata agacgcatag cgtcgcatcc gacaaccgat gcctgatgcg
     5821 ccgctgacgc ggcttatcag gcctacgggg cgtgctgaac tgtaggtcgg ataagacgta
     5881 tggcgtcgca tccgacaacc gatgcctgat gcgccgctga cgcgacttat caggcctacg
     5941 gggtgaactg aactgtaggt cggataagac gcatggcgtc gcatccgaca accgatgcct
     6001 gatgcgacgc tgacgcggct tatcaggcct gcggggtgaa ctgaactgta ggtcggataa
     6061 gacgcatagc gtcgcatccg acaacaatct cgaccctaca aatgataaca atgacgagga
     6121 caatatgagc gacacaacga tcctgcaaaa cagtacccat gtcattaaac cgaaaaaatc
     6181 tgtggcactt tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg gtaaaagtgg
     6241 caatgacttg cattaccgtg gttacgatat tattgatctg gcggaacatt gcgaatttga
     6301 agaagtggcg catctgctga tccacggcaa actgccgacc cgtgacgaac tcgccgctta
     6361 caaaacgaaa ctgaaagccc tgcgtggttt accagctaat gtgcgtaccg tgctggaagc
     6421 cttaccggcg gcgtcgcacc cgatggatgt tatgcgcacc ggcgtttccg cgctcggctg
     6481 cacgctgcca gaaaaagagg ggcataccgt ctctggcgcg cgggatattg ccgacaaact
     6541 gctggcgtca cttagctcga ttcttctcta ctggtatcac tacagccaca acggcgaacg
     6601 catccagccg gaaaccgatg acgactccat cggtggtcac ttcctgcacc tgctgcacgg
     6661 cgaaaagcca tcgcaaagct gggaaaaggc gatgcatatc tcgctggtgc tgtacgccga
     6721 acacgagttt aacgcctcca cctttaccag ccgggtgatt gcgggcactg gctctgatat
     6781 gtattccgcg attattggcg cgattggtgc actgcgcggg ccgaagcacg gcggggcgaa
     6841 tgaagtgtcg ctggagatcc agcaacgcta cgaaacgccg gacgaagccg aagccgatat
     6901 ccgcaagcgg gtggaaaaca aagaagtggt cattggtttt ggtcatccgg tttataccat
     6961 cgccgacccg cgtcatcagg tgatcaaacg tgtggcgaag cagctctcgc aggaaggcgg
     7021 ctcactgaag atgtacaaca tcgccgatcg cctggaaacg gtgatgtggg agagcaaaaa
     7081 gatgttcccc aatctcgact ggttctccgc tgtttcctac aacatgatgg gcgttcccac
     7141 cgagatgttc acaccactgt ttgttatcgc ccgcgtcacc ggctgggcgg cgcacattat
     7201 cgaacaacgt caggacaaca aaattatccg tccttccgcc aattatgttg gaccggaaga
     7261 ccgccagttt gtcgcgctgg ataagcgcca gtaaacctct acgaataaca ataaggaaac
     7321 gtacccaatg tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg aaatcgttga
     7381 tattgtcgat tacgtgatga actacgaaat cagctccaaa gtagcctacg acaccgcaca
     7441 ttactgcctg ctcgacacgc tcggctgcgg tcttgaagct ctcgaatacc cagcctgtaa
     7501 aaaactgctg gggccaattg tccccggcac cgtcgtacct aacggcgtgc gcgtccccgg
     7561 aactcagttc cagctcgacc ccgtccaggc ggcatttaac atcggcgcga tgatccgttg
     7621 gctcgatttc aacgatacct ggctggcggc ggagtggggg catccttccg acaacctcgg
     7681 cggcattctg gcaacggcag actggctttc gcgcaacgcg gtcgccagcg gcaaagcgcc
     7741 gttgaccatg aaacaggtgc tgaccgcaat gatcaaagcc catgaaattc agggctgcat
     7801 cgcgctggaa aactccttta accgcgtcgg cctcgaccac gttctgttag tgaaagtggc
     7861 ttccaccgcc gtggtcgccg aaatgctcgg cctgacccgc gaggaaattc tcaacgccgt
     7921 ttcgctggcg tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc cgaacaccgg
     7981 cacgcgtaaa tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc tggcactgat
     8041 ggcgaaaacg ggcgaaatgg gttacccgtc agccctgact gcgcctgtgt ggggcttcta
     8101 cgacgtctcc ttcaaaggtg agtcgttccg cttccagcgc ccgtacggtt cttacgtgat
     8161 ggaaaatgtg ctgttcaaaa tctccttccc ggcggagttc cactcccaga cggcagttga
     8221 agcagcgatg acgctctatg aacagatgca ggcagcaggc aaaacggcgg cagatatcga
     8281 aaaagtgacc attcgcaccc acgaagcctg tattcgcatc atcgacaaaa aaggaccgct
     8341 caataacccg gcagaccgcg accactgcat tcagtacatg gtggcgatcc cgctgctgtt
     8401 cggacgctta acggcggcag attacgagga caacgttgcg caagataaac gcatcgacgc
     8461 cctgcgcgag aagatcaatt gctttgaaga tccggcattt accgctgact accacgaccc
     8521 ggaaaaacgt gccatcgcca atgccataac ccttgagttc accgacggta cgcgatttga
     8581 agaagtggtg gtggagtacc ccattggtca tgctcgccgc cgtcaggatg gcattccgaa
     8641 gctggtcgat aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc aacagcagcg
     8701 cattctggag gtttctctcg acagagctcg cctggaacag atgccggtca atgagtatct
     8761 cgacctgtac gtcatttaag caaacagcgg taaggcgtaa gttcaacagg agagcattat
     8821 gtcttttagc gaattttatc agcgttcgat taacgaaccg gagcagttct gggccgagca
     8881 ggcccggcgt attgactggc agacgccctt tacgcaaacg ctcgatcaca gcaatccgcc
     8941 gtttgcccgt tggttttgtg aaggccgaac caacttgtgt cacaacgcta tcgaccgctg
     9001 gctggagaaa cagccagagg cgctggcgct gattgccgtc tcttcggaaa cagaagaaga
     9061 gtgcaccttt accttccgcc agttacatga cgaagtgaat gcggtggcct caatgctgcg
     9121 ctcgctgggt gtgcagcgcg gcgatcgggt gctggtgtat atgccgatga ttgccgaagc
     9181 gcatattact ctgctggctt gcgcgcgcat tggcgctatt cactcggtgg tgtttggggg
     9241 atttgcctcg cacagcgtgg cggcgcgaat tgatgatgct aaaccggtgc tgattgtctc
     9301 ggctgatgcc ggagcgcgcg gtggcaaaat cattccctat aaaaaattgc tcgacgatgc
     9361 gataagtcag gcgcagcacc agccacgcca tgttttgctg gtggatcgcg ggctggcgaa
     9421 aatggcacgc gtcagcgggc gggatgtcga tttcgcgtcg ttgcgccatc aacacatcgg
     9481 cgcgcgggta ccggtggcgt ggctggaatc caacgaaacc tcctgcattc tctacacctc
     9541 cggcacgacc ggcaaaccta aaggcgtgca gcgtgacgtt ggcggatatg cggtggcgct
     9601 ggcgacctcg atggacacca tttttggcgg caaagcgggc ggtgtgttct tttgtgcttc
     9661 ggatatcggc tgggtggtgg ggcattcgta tatcgtctac gcgccgctgc tggcggggat
     9721 ggcgactatc gtttacgaag gattaccgac ctggccggac tgcggcgtgt ggtggaaaat
     9781 cgtcgagaaa tatcaggtta gccggatgtt ctcagcgccg accgccattc gcgtgctgaa
     9841 aaaattccct accgctgaaa ttcgcaaaca cgatctctcg tcgctggaag tgctctatct
     9901 ggcgggggag ccgctggacg agccgaccgc cagttgggtg agcaatacgc tggatgtgcc
     9961 ggtcatcgac aactactggc agaccgaatc cggctggccg attatggcga ttgctcgcgg
    10021 tctggacgac aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg gctataacgt
    10081 gcagttgctt aatgaagtca ccggcgaacc gtgtggcgtc aacgagaaag ggatgctggt
    10141 ggtggaaggg ccattgccgc cggggtgtat tcagaccatt tggggcgacg acgaccgctt
    10201 tgtgaagacg tactggtcgc tgttttcccg tccggtgtac gccacttttg actggggtat
    10261 ccgcgatgct gacggttatc actttattct cgggcgcact gacgatgtga ttaacgttgc
    10321 cgggcatcgg ctggggacgc gcgagattga agagagtatc tccagtcatc cgggcgttgc
    10381 cgaagtggcg gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg tggcgtttgt
    10441 cattccgaaa gagagcgaca gtctggaaga ccgagacgtg gcgcactcgc aagagaaggc
    10501 gattatggcg ctggtggaca gccagattgg caactttggt cgcccggcgc acgtctggtt
    10561 tgtctcgcaa ttgccgaaaa cgcgatccgg aaaaatgctg cgccgcacga tccaggcgat
    10621 ttgcgaagga cgcgatcctg gggatctgac gaccattgat gatcctgcgt cgttggatca
    10681 gatccgccag gcgatggaag agtaaggtat tgtcggatgc gtcgtgtggc gcatccgaca
    10741 ccgtgcgccg atgcctgatg cgacgctgac gcgtcttatc atgcctacag gcctgaaccg
    10801 taggtcggat aaggcgctcg cgccgcatcc gacaccgtgc gccgatgcct gatgcgacgc
    10861 tggcgcgtct tatcatgcct acagg
//
LOCUS       DAYPHN010000157        10626 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000157 DAYPHN010000000
VERSION     DAYPHN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.157"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            88..798
                     /locus_tag="ACG0BW_003904"
     CDS             88..798
                     /locus_tag="ACG0BW_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFK7260806.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(848..1822)
                     /gene="ghrB"
                     /locus_tag="ACG0BW_003905"
     CDS             complement(848..1822)
                     /gene="ghrB"
                     /locus_tag="ACG0BW_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFK7260807.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAIFA
                     EAEGLLGSNENVDAALLGKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDSLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1926..2585)
                     /locus_tag="ACG0BW_003906"
     CDS             complement(1926..2585)
                     /locus_tag="ACG0BW_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFK7260808.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            2738..5071
                     /gene="bisC"
                     /locus_tag="ACG0BW_003907"
     CDS             2738..5071
                     /gene="bisC"
                     /locus_tag="ACG0BW_003907"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFK7260809.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVLSSRGALTTGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPEKPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVEFFGDKMEWVAPHMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVNAEKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGQSELQWLETFYNVARQRGASQQVELPPFAEFWKANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(5040..5480)
                     /locus_tag="ACG0BW_003908"
     CDS             complement(5040..5480)
                     /locus_tag="ACG0BW_003908"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFK7260810.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKFLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(5477..6040)
                     /gene="tag"
                     /locus_tag="ACG0BW_003909"
     CDS             complement(5477..6040)
                     /gene="tag"
                     /locus_tag="ACG0BW_003909"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFK7260811.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            6198..6896
                     /locus_tag="ACG0BW_003910"
     CDS             6198..6896
                     /locus_tag="ACG0BW_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFK7260812.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQTENTTN
                     NSDYLYTMGVSARF"
     gene            7125..8333
                     /locus_tag="ACG0BW_003911"
     CDS             7125..8333
                     /locus_tag="ACG0BW_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7260813.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGIVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            8657..10348
                     /gene="eptB"
                     /locus_tag="ACG0BW_003912"
     CDS             8657..10348
                     /gene="eptB"
                     /locus_tag="ACG0BW_003912"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFK7260814.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYSLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            10440..10516
                     /locus_tag="ACG0BW_003913"
     tRNA            10440..10516
                     /locus_tag="ACG0BW_003913"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10474..10476,aa:Pro,seq:cgg)
BASE COUNT         2432 a         2643 c         2826 g         2725 t
ORIGIN      
        1 atcctgcaag cttgtcctgg actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg
       61 ctttttttgg gtgaaaggag taagaaaatg gcgacaggaa agtcctgctc tcgctggttt
      121 gcgcctcttg cggcgttatt aatggtagtt agcctgagtg ggtgttttga taaagaaggc
      181 gatcagcgta aagcgtttat cgacttcctg cagaatacag tgatgcgtag cggtgaacgt
      241 ctacctaccc tgactgccga tcagaaaaaa caatttggtc cttttgtctc tgattacgcg
      301 attctgtatg gttattcaca gcaggtgaat caggcgatgg attccggtct gcgcccggtt
      361 gtagacagcg ttaacgcaat tcgcgtaccg caggattacg ttacgcaaag cggcccactg
      421 cgtgaaatga acggttctct gggtgtactg gcgcaacagc tgcaaaatgc gaagttgcag
      481 gccgatgctg cgcactctgc gctaaaacag agcgatgacc tgaaaccggt cttcgatcag
      541 gcattcacca aagtggtaac gacgccagct gatgcgttgc aaccgttgat tccggcggca
      601 caaactttca cgcaacaact ggtgatggtt ggagactaca ttgctcagca aggtactcag
      661 gtgagctttg tcgcaaatgg catccagttc ccgacttcgc agcaggcaag tgaatataac
      721 aaactgattg cgccattacc ggcacagcat caggcgttta atcaggcctg gactacagca
      781 gttactgcaa cccaataaag agtaaaagcc cgagcgatta tcgggcttta ctccacgcag
      841 tcgcggctta gtccgcgacg tgcggattta cacagttctt ctcaaccttt ccttgtaacg
      901 catcaatcaa gttatccacg gcacaggcag ccatgccata acgcgtctca tgagtggctg
      961 aaccaatatg cggtaccgcg acgacgttgg ccattgagag caacggcgaa tctacagaca
     1021 gtggctcttg ttcgaagaca tccagcccgg cggcgtgaat ttcgcctttc tgcaatgcag
     1081 caatcagtgc attttcatca accaccgggc cacgtccggc attaatgaaa atggcggagg
     1141 atttcatttt gccgaattgt tctgcgccaa atagatgata cgtctcatca gttaacggca
     1201 ggatcaggca aacgaaatct gactcttgta acagtgaatc caaatcgcag tagcgggcgt
     1261 tgaagcgttc ttctgcttct ttatggtggc ggcgcgcgtt atagaggatg ggcatattga
     1321 agccaaagtg cgcacgttgt gccagcgcca tgccgatccg tcccatcccg acaatgccca
     1381 gtgttttatg gtgaacgtca gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc
     1441 ctgcttttac ccgttctgct acctccacaa cccgacgagc ggtagacaac accagcgcca
     1501 tcagcgtatc ggcgacggtt tctgttaata cggttggcgt gtgcatcagc agaatttttc
     1561 gggcggtaag cgcatcgaca tcaaaattgt catagccgac ggagatcgtt gatgtggcac
     1621 gcagtttcgg catttttccc agcaatgcgg catcaacatt ctcgtttgaa cccagtaaac
     1681 cttcagcttc ggcaaaaatt gctgcatttt gttcgatggt ttgtgggctg aggtttgcca
     1741 cctggtgaac ggtgaaatgc tcttgcaggc gttgcagtaa atcatcaggt aaggctttgt
     1801 agaggataac ggacggcttc atgcttctct ccattcactg ataaggcgat caccttagcc
     1861 tgaaatatga tgaaggaaaa ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa
     1921 agggattaca gcgggcttaa ggtaatttct acacggcggt tttgcgcctt accttctgcg
     1981 gtgctgttgc tggcgattgg gttagccggg ccaaggccct gagtacggat gcggctggcg
     2041 tccacgccct gggtgatcaa cgcgctggca acggaatccg cacgctgctg ggagagacgc
     2101 atgttcaggt cgtgaccacc cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc
     2161 ggatactctt tcagcaccat tgccacgccg gtcagggtgt tagcgcctgc cggtttcagg
     2221 gtcgcgctgc tgctgtcgaa ggtcacattg ttcggcatat tgaggataat gttatccccg
     2281 ctgcgggtta cgctaacacc agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca
     2341 tccatgtaat aacccacgcc gccgcccaaa gctgcgcctg ctgctgcgcc aatcagcgcg
     2401 cctttaccgc gatctttctt cgaagaagag agcgcaccaa tacccgcgcc aacgagagag
     2461 cccagacctg cgccgatagc agatttacct gcttcgcgtt cgccagtgta agggttagtt
     2521 gtgcagccag ataccgccag agcgccactc actacggcgg caataagata aacacgtttc
     2581 ttcattgtta atccttaata acctttttat tctttgccac gggttccgtg gcgggagatt
     2641 atgccgcgtg aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta
     2701 actgctcaat acatctaacc tttcaggagc cttcggtttg gccaactcat cctcacgata
     2761 ttccgttctg actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt
     2821 gcttagctcg cgtggcgcgt taaccacagg aatggaaaac tccttgcaga gcgcggttcg
     2881 cgaccaggtt cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttcttgc
     2941 gtcaccggaa aaaccgcaag gcattcgcgg gcaggatgaa tttgttcgtg tgagttggga
     3001 tgaggcgctg gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc
     3061 atcgattttt gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac
     3121 attattacaa cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc
     3181 gaccggtgca gcacaggcga tcatgccgta tgtcgtgggg ggcagcgaag tttatcaaca
     3241 gcaaaccagt tggccgttgg tgctggaaca tagcgatgtc gtggtgctgt ggagtgctaa
     3301 cccactcaat acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt
     3361 ttctgcactg cgtgacagcg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac
     3421 cgttgagttc tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc
     3481 gctgatgctg gggatcgcct atacgctggt ggaaaatggt tggcacgacg aagcgtttct
     3541 ggcgcgttgc accaccggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg
     3601 aatagccaaa accgccgaat gggcggcagg gatttgtggg gttaacgcag agaaaatccg
     3661 cgagctggcg gctcttttcc accaaaacac gaccatgctg atggctggct ggggaatgca
     3721 gcgtcaacag tttggtgagc aaaaacattg gatgatcgtc acgctggcgg caatgttggg
     3781 gcaaatcggc acacccggcg gcggttttgg tctttcttat cattttgcca atggtggtaa
     3841 ccccacgcgg cgttctgcgg tgctctcttc catgcagggc agcttgcctg gcggcaccga
     3901 tgcggtagat aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc
     3961 atatcaacac aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg
     4021 cggtgccaac tttactcatc atcaggatac caatcgcctg atccgtgcct ggcaaaaacc
     4081 ggagctggtg gtgatctctg aatgcttctg gacggctgcg gcgaaacacg cggatatcgt
     4141 tctgcctgcg actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa
     4201 tcagcatctg gtgccgatga aacaagtggt gccgccacgt tatgaagcgc gtaatgattt
     4261 tgatgtcttt gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga
     4321 aggacaaagt gagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc
     4381 aagccagcag gttgaattgc cgccatttgc tgagttctgg aaagccaacc agttaattga
     4441 gatgccggaa aacccggaca gtgagcggtt tattcgcttc gccgattttc gccgcgatcc
     4501 gcaggcgcat ccattaaaaa ccgccagcgg caagattgaa atcttctcgc agcgtattgc
     4561 cgattacgct tatccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca
     4621 gggcaatgcc gaaccggaac agttgcaggt actttctgct catccggcac atcgtctgca
     4681 cagccagctg aattacagtt ctctgcgcga attgtacgcg gtggcaaatc gtgagcctgt
     4741 caccattcat cctgacgatg cccaggcgcg cggcatacaa gatggcgata ttgttcggtt
     4801 gtggaacgca cgcgggcaaa ttcttgccgg agcggtcatt agcgagggaa ttaaacctgg
     4861 cgtgatttgt attcatgaag gggcttggcc ggatctggat ttaaccgctg acggtatttg
     4921 taaaaacggc gcggtgaacg ttctgaccaa agatctcccc agctcgcggc tggggaatgg
     4981 ctgtgcgggt aatacggcgc tggcatggct ggaaaaatac aacggtccgg aactgacact
     5041 tacagcgttt gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc
     5101 catgcgcagt cgacaatgtg aaaaccctgc gcatggtaga aatctatcgc cggttgattt
     5161 ttttgataaa cctccagcat cagatgggga tggcgctgct gcacatactg catcagcgcc
     5221 ttaccaatac cgcgtctgac ggccttcggt gcgacaaaca ttgcagccag aaatcggcct
     5281 tccataatgc tgacaaaacc gagaaactta ccgtcttctt cccagaccca gttttgcgcg
     5341 ttggcaagat aggcatcccg caccagcgga atgcaatcac gccagtaatt cgcttttata
     5401 aagggatgcc cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa
     5461 cgttgcgctt cccgaatcat ggtttatttc ccggatagca acagcagcca accacatgat
     5521 cattcaccag cccacatgcc tgcataaagg agtaacagat tgtggtacca acaaacttaa
     5581 aaccacgctt tttcagggct ttagatagag cgtcggaggc gggcgtagat gtagggattt
     5641 cgctcaacgt tgtggcttgt gtcacctgtg gttgatgatt gacaaacgac cagacaaagt
     5701 cggcaaacgg ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg
     5761 cctgaatttt cccgcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct
     5821 cttcctgcat tgcagccacc ttcaccggat cgaactgatg aaagctggcg cgatagtttt
     5881 cgcgtttttt gaggacggtg atccacgata acccagcctg ctgcccttca aggcagatca
     5941 tttcgaacag ttttttactg tcagtttcag gcacgcccca ttcattatca tggtaggcaa
     6001 tataaagcgg gtcctgactc acccagccgc aacgttccat actttccctc gctatgtacc
     6061 cgatcgtaaa aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata
     6121 cagggctact atgcccgata tccttcatac aatgacaata atattgccga attcggctct
     6181 tccctggagt cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc
     6241 tggcgagtgt ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat
     6301 atatcgttga tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc
     6361 aacctgatta caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac
     6421 tggaagtcaa tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct
     6481 ggaattttcc tttatatgag caggttacaa ccggcccggt cgcggcatta cattacgatg
     6541 gcacaaccac ctcgatgtat aacgagtttg gcgacagtac taccacgctg gccgatccgt
     6601 tatggcatgc cagcgtgagt accttaggct ggcgtgttga ctcccggctt ggcgatctcc
     6661 gaccctgggc gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat
     6721 caggcctgag ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag
     6781 gcgctgatat gttgctcaat caaaatattg ctgcctatgc ggcgctatct cagacagaaa
     6841 ataccactaa caatagcgac tatctgtata cgatgggggt tagtgccaga ttttaacgtt
     6901 atagatacaa ttgaaaccat tggataacaa cagttgtggc gatagtgggt gctaacttac
     6961 taaataataa atttggtgaa taattgtcga gtcattcatt cctgaactaa tgcatttcat
     7021 tccgttctga tggcatttca tgccgttttt ccccaggcat aaagtgcact tcgttatggt
     7081 tgtcagcaga gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt
     7141 atcagcgtac ccgctggttg acactcatcg gtactatcat tacccagttt gcgctggggt
     7201 cggtttatac ctggagcctg tttaacggcg cgctttctgc caagctggat gcgccggtaa
     7261 gccaggtcgc tttctctttc ggcttgttaa gtctggggct ggcaatttca tcttctgttg
     7321 cggggaaatt acaggaacgt tttggcgtta aacgcgtcac tatggcttcc ggcattttgc
     7381 tgggattagg cttcttcctg acagcgcatt ctgacaacct gatgatgctg tggttaagcg
     7441 ccggtgtgct ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg
     7501 tgaagtggtt cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc
     7561 tgggtagcct ggggttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa
     7621 aaacatttgt gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa
     7681 tgaaagacgc accaaaacag gaagtgaaaa ccagcaatgg tatcgtggag aaagattaca
     7741 cgctggcaga gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg
     7801 cctgcatgag cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac
     7861 accttgatgt ggtttccgca gccaatgcag tcactgttat ttccatcgcc aacctttcag
     7921 gtcgtctggt tctggggata ctgtctgaca aaatcgcccg aatccgtgtt atcaccattg
     7981 gtcaggtgat ttcgctggtg ggtatggcgg ccctgctgtt tgcaccattg aatgcagtga
     8041 cgttctttgc agcgattgcc tgcgtggcgt ttaactttgg cggcactatc acggtgttcc
     8101 cgtcactggt cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt
     8161 atctcggttt cggtatcggc agcatttgtg ggtcgattat cgcctcactg tttggcggct
     8221 tctatgtgac cttctacgtg attttcgccc tgctgattct gtcactggcg ctctctacga
     8281 cgattcgtca gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatcatctg
     8341 ccgcgagcta ctgggcttta gccttagtgt gaatcaaacg cttcaacgca atgtataaac
     8401 aatgacgcgt aaaatttggg ccagttaact ggcccttttt actgcttttt gtaacgctaa
     8461 agtctctttt caaacttgca tttttgtaaa tttgtgcttc gtgcacactc tttccccaca
     8521 ctttttccct ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc
     8581 tccgcacttt tcccctgccg ggcctgaaaa gccgttaagc agggtgttat cacctgtttg
     8641 tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt
     8701 gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttaccgcc gcttcggcag
     8761 ctatgcgcac gattttaccg tctggaaagg catttctgct gttgttgaac tggctgccac
     8821 cgtactggta accttctttt tactgcgtct tctttcgctg tttggccgcc gtagctggcg
     8881 tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct
     8941 taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc
     9001 aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagcg cattgcctct
     9061 tatccttatc tggaataacc gctgtcgcta ctccttgctc cgacaactgc gaaccccagg
     9121 gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt gggcaccgat
     9181 tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggtgttg atttgcccag
     9241 ctatggcggc gtcgtagcga actcttatct gccatcaaac tggctttcgg cattggggct
     9301 gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta acccggcgaa
     9361 gaaattcact taccaggcac cgcaaaacgt tgatgacact tatgtcgtgt ttatcattgg
     9421 tgaaaccacg cgttgggacc atatgggcat tttcggctat gagcgtaata ccacgccgaa
     9481 gctggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaactaa
     9541 actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac
     9601 attaaaagaa cagaacattt tcgcggtgct gaagcagtta ggattcagtt ctgatctcta
     9661 cgctatgcaa agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg
     9721 tgagcagatt ggtgcggagc cacgtaaccg tggcaagccg gtagatgata tgttgctggt
     9781 agacgaaatg cagcaatcgc tggggcgcaa cccggatggc aagcatctga tcattttgca
     9841 taccaaaggt tcgcacttta actacaccca gcgttacccg cgcagctttg cgcagtggaa
     9901 gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga
     9961 caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa
    10021 gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct
    10081 gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtgccga tgatggtctg
    10141 gatgtcagat aaatatctgg aaaatccggt caatgcgcag gcatttgcgc agctgaaaaa
    10201 agaagccgac atgaaagtgc cacgccgtca tgtagagctg tacgacacca tcatggggtg
    10261 tcttggctat acttcacctg atggtggaat taacgaaaac aacaactggt gccacatccc
    10321 gcaggcaaga gaggcggcgg ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg
    10381 cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc
    10441 ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat
    10501 cctctatcac cgaccaaatt cgaaatgcct gctcagcgag caggcttttt tgcatttgta
    10561 gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag gcctgataag
    10621 acgcgc
//
LOCUS       DAYPHN010000158        10615 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000158 DAYPHN010000000
VERSION     DAYPHN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.158"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            269..673
                     /gene="sdhC"
                     /locus_tag="ACG0BW_003914"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             269..673
                     /gene="sdhC"
                     /locus_tag="ACG0BW_003914"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFK7260815.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            667..1014
                     /gene="sdhD"
                     /locus_tag="ACG0BW_003915"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             667..1014
                     /gene="sdhD"
                     /locus_tag="ACG0BW_003915"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFK7260816.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1014..2780
                     /gene="sdhA"
                     /locus_tag="ACG0BW_003916"
     CDS             1014..2780
                     /gene="sdhA"
                     /locus_tag="ACG0BW_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFK7260817.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPEAESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2796..3512
                     /gene="sdhB"
                     /locus_tag="ACG0BW_003917"
     CDS             2796..3512
                     /gene="sdhB"
                     /locus_tag="ACG0BW_003917"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFK7260818.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEADEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3862..6663
                     /gene="sucA"
                     /locus_tag="ACG0BW_003918"
     CDS             3862..6663
                     /gene="sucA"
                     /locus_tag="ACG0BW_003918"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFK7260819.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6678..7895
                     /gene="odhB"
                     /locus_tag="ACG0BW_003919"
     CDS             6678..7895
                     /gene="odhB"
                     /locus_tag="ACG0BW_003919"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFK7260820.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALVARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGLVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8170..9336
                     /gene="sucC"
                     /locus_tag="ACG0BW_003920"
     CDS             8170..9336
                     /gene="sucC"
                     /locus_tag="ACG0BW_003920"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFK7260821.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9336..10205
                     /gene="sucD"
                     /locus_tag="ACG0BW_003921"
     CDS             9336..10205
                     /gene="sucD"
                     /locus_tag="ACG0BW_003921"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFK7260822.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            10346..10600
                     /locus_tag="ACG0BW_003922"
     CDS             10346..10600
                     /locus_tag="ACG0BW_003922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260823.1"
                     /translation="MTAWPQPLWIAATDRKPRIRTTANFESLPDALASQRLTVALSKS
                     TKAMFTAYRQVVPFVSQDRVFTPDIAASQKFLEGYAINQK"
BASE COUNT         2509 a         2770 c         2960 g         2376 t
ORIGIN      
        1 aacccgacaa actatatgta ggttaattgt aatgattttg tgaacggcct atactgccgc
       61 caggtctccg gaacaccctg caatcccgag ccacccagcg ttgtaacgtg tcgttttcgc
      121 atctggaagc agtgttttgc atgacgcgca gttatagaaa ggacgctgtc tgacccgcaa
      181 gcagaccgga ggaaggaaat cccgacgtct ccaggtaaca gaaagttaac ctctgtgccc
      241 gtagtcccca gggaataata agaacagcat gtgggcgtta ttcatgataa gaaatgtgaa
      301 aaaacaaaga cctgttaatc tggacctaca gaccatccgg ttccccatca cggcgatagc
      361 gtccattctc catcgcgttt ccggtgtgat cacctttgtt gcagtgggca tcctgctgtg
      421 gcttctgggt accagcctct cttcccctga aggtttcgag caagcttccg cgattatggg
      481 cagcttcttc gtcaaattta tcatgtgggg catccttacc gctctggcgt atcacgtcgt
      541 cgtaggtatt cgccacatga tgatggattt tggctatctg gaagaaacat tcgaagcggg
      601 taaacgctcc gccaaaatct cctttgttat tactgtcgtg ctttcacttc tcgcaggagt
      661 cctcgtatgg taagcaacgc ctccgcatta ggacgcaatg gcgtacatga tttcatcctc
      721 gttcgcgcta ccgctatcgt cctgacgctc tacatcattt atatggtcgg ttttttcgct
      781 accagtggcg agctgacata tgaagtctgg atcggtttct tcgcctctgc gttcaccaaa
      841 gtgttcaccc tgctggcgct gttttctatc ttgattcatg cctggatcgg catgtggcag
      901 gtgttgaccg actacgttaa accgctggcc ttgcgcctga tgctgcaact ggtgattgtc
      961 gttgcactgg tggtttacgt gatttatgga ttcgttgtgg tgtggggtgt gtgatgaaat
     1021 tgccagtcag agaatttgat gcagttgtga ttggtgccgg tggcgcaggt atgcgcgcgg
     1081 cgctgcaaat ttcccagagc ggtcagacct gtgcgctgct ctctaaagtc ttcccgaccc
     1141 gttcccatac cgtttctgcg caaggcggca ttaccgttgc gctgggtaat acccatgaag
     1201 ataactggga atggcatatg tacgataccg taaaagggtc ggactatatc ggtgaccagg
     1261 acgcgattga atatatgtgt aaaaccgggc cggaagcgat tctggaactc gaacacatgg
     1321 gcctgccgtt ctcgcgtctt gatgatggtc gtatctatca acgtccgttt ggcggtcagt
     1381 cgaaaaactt cggcggcgag caggcggcac gcactgcggc ggctgctgac cgtaccggtc
     1441 acgcactgtt gcacaccctt tatcagcaga acctgaaaaa ccacaccacc attttctccg
     1501 agtggtatgc gctggatctg gtgaagaacc aggatggcgc agtggttggt tgtaccgcat
     1561 tgtgcatcga aactggtgaa gtggtttatt tcaaagcccg cgctaccgtg ctggcgactg
     1621 gcggggcagg gcgtatttat cagtccacca ctaacgccca cattaacact ggcgacggcg
     1681 tcggcatggc tatccgtgca ggcgtaccag tgcaggatat ggaaatgtgg cagttccacc
     1741 cgaccggcat tgcaggtgcg ggcgtactgg tcaccgaagg ttgccgtggt gaaggcggtt
     1801 atctgctgaa caaacatggc gaacgcttta tggagcgtta tgcgccgaac gccaaagacc
     1861 tggcgggccg tgatgtggtg gcgcgttcca tcatgattga aatccgtgaa ggtcgcggct
     1921 gtgatggtcc gtgggggcca cacgcgaaac tgaaactcga tcacctgggt aaagaagttc
     1981 tcgaatcccg tctgccgggt atcctggaac tctcccgtac cttcgctcac gttgatccgg
     2041 tgaaagagcc gattccggtt atcccaacct gtcactacat gatgggcggt attccgacca
     2101 aagtgaccgg tcaggcgctg acggtgaacg agaaaggcga agatgtggtt gttccggggc
     2161 tgtttgccgt tggtgaaatc gcttgtgtat cggtacacgg cgctaaccgt cttggcggca
     2221 actcgctgct ggacctggtg gtctttggtc gcgcagcggg tctgcatctg caagagtcta
     2281 ttgctgagca gggcgcactg cgcgatgcca gcgagtctga tgtagaagcg tctctggatc
     2341 gtctgaatcg ctggaacaac aaccgtaacg gtgaagatcc ggtggcgatc cgtaaagcac
     2401 tgcaagaatg tatgcagcat aacttctcgg tcttccgtga aggtgatgcg atggcgaaag
     2461 ggcttgagca gttgaaagtg atccgcgagc gtctgaaaaa tgcccgtctg gatgacactt
     2521 ccagcgagtt caacacccag cgcgttgagt gcctggaact ggataacctg atggaaacgg
     2581 cgtatgcaac agctgtttct gccaacttcc gtaccgaaag ccgtggcgcg catagccgct
     2641 tcgatttccc ggatcgtgat gatgaaaact ggctgtgcca ctccctgtat ctgccagagg
     2701 cggaatccat gacgcgccga agcgtcaaca tggaaccgaa actgcgcccg gcattcccgc
     2761 cgaagattcg tacttactaa tgcggagaca ggaaaatgag actcgagttt tcaatttatc
     2821 gctataaccc ggatgttgat gatgctccgc gtatgcagga ttacaccctg gaagcggatg
     2881 aaggtcgcga catgatgctg ctggatgcgc ttatccagct gaaagagaaa gatcccagcc
     2941 tgtcgttccg ccgctcctgc cgtgaaggtg tgtgcggttc cgacggtctg aacatgaacg
     3001 gcaagaatgg tctggcctgt attaccccga tttcggcact caaccagccg ggcaagaaga
     3061 ttgtgattcg cccgctgcca ggtttaccgg tgatccgcga tttggtggta gacatgggac
     3121 aattctatgc gcaatatgag aaaattaagc cttacctgtt gaataatgga caaaatccgc
     3181 cagctcgcga gcatttacag atgccagagc agcgcgaaaa actcgacggg ttgtatgaat
     3241 gtattctctg cgcatgttgt tcaacctctt gtccgtcttt ctggtggaat cccgataagt
     3301 ttatcggccc ggcaggcttg ttagcggcat atcgtttcct gatcgatagc cgtgataccg
     3361 agactgacag ccgcctcgac ggtttgagcg atgcattcag cgtattccgc tgtcacagca
     3421 tcatgaactg cgtcagtgta tgtccgaagg ggctgaaccc gacgcgcgcc atcggccata
     3481 tcaagtcgat gttgttgcag cgtaatgcgt aaacccaggc ctgataagac gcacaagcgt
     3541 cgcatcaggc aaccagtgcc ggatgcggcg tgaacgcctt atccggccta caaaagaaat
     3601 gcagaaatcg taggcctgat aagacgcgca agcgtcgcat caggcaaaca gcgccggatg
     3661 cggcgtgaat gccttatccg gcctacaaaa gaaatgcagg aaatctttaa aaactgcccc
     3721 tgacactaag acagttttta aaggttcctt cgcgagccac tacgtagaca agagctcgca
     3781 agtgaacccc ggcacgcaca tcactgtgcg tggtagtatc cacggcgaag taagcataaa
     3841 aaagatgctt aagggatcac gatgcagaac agcgctttga aagcctggtt ggactcttct
     3901 tacctctctg gcgcaaacca gagctggata gaacagctct atgaagactt cttaaccgat
     3961 cctgactcgg ttgacgctaa ctggcgttcg acgttccagc agttacctgg tacgggagtc
     4021 aaaccggatc aattccactc tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct
     4081 tcacgttact cttcaacgat ctccgaccct gacaccaatg tgaagcaggt taaagtcctg
     4141 cagctcatta acgcataccg cttccgtggt caccagcatg cgaatctcga tccgctggga
     4201 ctgtggcagc aagataaagt ggccgatctg gatccgtctt tccacgatct gaccgaagca
     4261 gacttccagg agaccttcaa cgtcggttca tttgccagcg gcaaagaaac catgaaactc
     4321 ggcgagctgc tggaagccct caagcaaacc tactgcggcc cgattggtgc cgagtatatg
     4381 catattacca gcaccgaaga aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg
     4441 actttcaata gcgaagagaa aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt
     4501 gaacgttacc tcggcgcaaa attccctggc gcaaaacgct tctcgctgga aggcggtgac
     4561 gcgttaatcc cgatgcttaa agagatgatc cgccacgctg gcaacagcgg cacccgcgaa
     4621 gtggttctcg ggatggcgca ccgtggtcgt ctgaacgtgc tggtgaacgt gctgggtaaa
     4681 aaaccgcaag acttgttcga cgagtttgcc ggtaaacata aagaacacct cggcacgggc
     4741 gacgtgaaat accacatggg cttctcgtct gacttccaga ccgatggcgg cctggtgcac
     4801 ctggcgctgg cgtttaaccc gtctcacctt gagattgtaa gcccggtagt tatcggttct
     4861 gttcgtgccc gtctggacag acttgatgag ccgagcagca acaaagtgct gccaattacc
     4921 atccacggtg acgccgcagt gaccgggcag ggcgtggttc aggaaaccct gaacatgtcg
     4981 aaagcgcgtg gctatgaagt tggcggtacg gtacgtatcg ttatcaacaa ccaggttggc
     5041 ttcaccacct ctaacccgct ggatgcccgt tcgacgccgt actgtactga tatcggtaag
     5101 atggttcagg caccgatttt ccacgttaac gcggatgatc ctgaagccgt tgcctttgtg
     5161 acccgtctgg cgctcgattt ccgtaacacc tttaaacgtg atgtcttcat cgacctggtg
     5221 tgctaccgcc gtcatggcca taacgaagcc gacgagccga gcgcaaccca gccgctgatg
     5281 tatcagaaaa tcaaaaaaca tccgacgccg cgcaaaatct acgctgacaa gctggagcaa
     5341 gaaaaagtcg cgacgctgga agatgccacc gagatggtta acctgtaccg cgatgcgctg
     5401 gatgctggcg attgcgtggt agcagagtgg cgtccgatga acatgcactc tttcacctgg
     5461 tcaccgtacc tcaaccacga atgggacgaa gagtacccga acaaagttga gatgaagcgc
     5521 ctgcaggaac tggctaaacg catcagcacg gtgccggaag cagttgaaat gcagtctcgc
     5581 gttgccaaga tttatggcga tcgccaggcg atggctgccg gtgagaaact gttcgactgg
     5641 ggcggcgcgg aaaacctcgc ttacgccacg ctggttgacg aaggcattcc ggttcgcctg
     5701 tcgggtgaag actccggtcg cggtaccttc ttccaccgcc acgcggtgat ccacaaccag
     5761 tctaacggtt ccacttacac gccgctgcaa cacatccata acggccaggg cgcgttccgt
     5821 gtctgggact ccgtactgtc tgaagaagcc gtgctggcgt ttgaatacgg ttatgccacc
     5881 gcagaaccac gcactctgac tatctgggaa gcacagttcg gtgacttcgc taacggtgct
     5941 caggtggtta tcgaccagtt catctcctct ggcgagcaga aatggggccg gatgtgtggt
     6001 ctggtgatgt tgctgccgca cggttacgaa gggcaggggc cggagcactc ctccgcgcgt
     6061 ctggaacgtt atctgcaact ttgtgctgag caaaacatgc aggtttgcgt accttctacc
     6121 ccggcacagg tttaccacat gctgcgtcgt caggcgctgc gcgggatgcg tcgtccgctg
     6181 gtcgtgatgt cgccgaaatc cctgctgcgt catccgctgg cggtttccag cctcgaagaa
     6241 ctggcgaacg gcaccttcct gccagccatc ggtgaaatcg acgagcttga tccgaagggc
     6301 gttaagcgtg tagtgatgtg ttctggtaag gtttattacg acctgctgga acaacgtcgt
     6361 aagaacaatc aacacgatgt cgccattgtg cgtatcgagc aactctaccc gttcccgcat
     6421 aaagcgatgc aggaagtgtt gcagcagttt gctcacgtca aggattttgt ctggtgccag
     6481 gaagaaccgc tcaaccaggg cgcatggtac tgcagccagc atcatttccg tgaagtgatt
     6541 ccgtttgggg cttctctgcg ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg
     6601 tatatgtccg ttcaccagaa acagcaacaa gatctggtta atgacgcgct gaacgtcgaa
     6661 taaataaagg atacacaatg agtagcgtag atattctggt ccctgacctg cctgaatccg
     6721 tagccgatgc caccgtcgca acctggcata aaaaacccgg cgacgcagtc gtacgtgatg
     6781 aagtgctggt agaaatcgaa actgacaaag tggtactgga agtaccggca tcagcagacg
     6841 gcattctgga tgcggttctg gaagatgaag gtacaacggt aacgtctcgt cagatccttg
     6901 gtcgcctgcg tgaaggcaac agcgccggta aagaaaccag cgccaaatct gaagagaaag
     6961 cgtccactcc ggcgcaacgc cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa
     7021 gcccggcgat ccgtcgcctg ctggctgaac acaacctcga cgccagcgcc attaaaggca
     7081 ccggtgtggg tggtcgtctg acccgtgaag atgtggaaaa acatctggcg aaagccccgg
     7141 cgaaagagtc tgctccggca gcggctgctc cggcggcgca accggctctg gttgcacgta
     7201 gcgaaaaacg tgtcccgatg actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag
     7261 cgaaaaactc caccgccatg ctgaccacgt tcaacgaagt caacatgaag ccgattatgg
     7321 atctgcgtaa gcagtacggt gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta
     7381 tgtccttcta cgtgaaagcg gtggttgaag ccctgaaacg ttacccggaa gtgaatgctt
     7441 ctatcgacgg cgatgacgtg gtttaccaca actatttcga cgtcagcatg gcggtttcta
     7501 cgccgcgcgg cctggtgacg ccggtactgc gtgatgtcga taccctcggc atggcagaca
     7561 tcgagaagaa aatcaaagag ctggcagtca aaggccgtga tggcaagctg acggttgaag
     7621 atctgaccgg tggtaacttc accatcacca acggtggtgt gttcggttcc ctgatgtcta
     7681 cgccgatcat caacccgccg cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc
     7741 cgatggcggt gaatggtctg gttgagatcc tgccgatgat gtacctggcg ctgtcctacg
     7801 atcaccgtct gatcgatggt cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc
     7861 tggaagatcc gacgcgtctg ctgctggacg tgtagtagtt taagcttcac ctgcactgta
     7921 gaccggatga ggcattatcg ccttctccgg caattgaagc ctgatgcgac gctgacgcgt
     7981 cttatcaggc ctacggggac catcaatgta ggtcggataa ggcgctcgcg ccgcatccga
     8041 cacgcgatgc ctgatgcgac gcttgcgcgt cttatcaggc ctacgggtga ccgacaatac
     8101 ccggaagcga tacgaaatat tcggtctacg gtttaaaaga taacgattac tgaaggatgg
     8161 acagaacaca tgaacttaca tgaatatcag gcaaaacaac tttttgcccg ctatggctta
     8221 ccagccccgg tgggttatgc ctgtactact ccgcgcgaag cagaagaagc cgcttcaaaa
     8281 atcggtgccg gtccgtgggt agtgaaatgt caggttcacg ctggtggccg cggtaaagcg
     8341 ggcggtgtga aagttgtaaa cagcaaagaa gacatccgtg cttttgcaga aaactggctg
     8401 ggcaagcgtc tggtaacgta tcaaacagat gccaatggcc aaccggttaa ccagattctg
     8461 gttgaagcag cgaccgatat cgctaaagag ctgtatctcg gtgccgttgt agaccgtagt
     8521 tcccgtcgtg tggtctttat ggcatccacc gaaggcggcg tggaaatcga aaaagtggcg
     8581 gaagaaactc cgcacctgat ccataaagtt gcgcttgatc cgctgactgg tccgatgccg
     8641 tatcagggac gcgagctggc gttcaaactg ggtctggaag gtaaactggt tcagcagttc
     8701 accaaaatct tcatgggcct ggcgaccatt ttcctggagc gcgacctggc gctgatcgaa
     8761 atcaacccgc tggtcattac caaacagggc gatctgattt gcctcgacgg caaactgggc
     8821 gctgatggca acgcactgtt ccgccagcct gatctgcgtg aaatgcgtga ccagtcgcag
     8881 gaagatccac gtgaagcaca ggctgcacag tgggaactga actacgttgc gctggacggt
     8941 aacatcggtt gtatggttaa cggcgcaggt ctggcgatgg gtacgatgga catcgttaaa
     9001 ctgcacggcg gcgaaccagc taacttcctg gacgttggcg gcggcgcaac caaagagcgc
     9061 gtaaccgaag cgttcaaaat catcctctct gacgacaaag tgaaagccgt tctggttaac
     9121 atcttcggcg gtatcgttcg ttgcgacctg atcgctgacg gtatcatcgg cgcggtagcg
     9181 gaagtgggtg ttaacgtacc ggtcgtggta cgtctggaag gtaacaacgc cgaactcggc
     9241 gcgaagaaac tggctgacag cggcctgaat attattgcag caaaaggtct gacggatgca
     9301 gctcagcagg ttgttgccgc agtggagggg aaataatgtc cattttaatt gataaaaaca
     9361 ccaaggttat ctgccagggc tttaccggta gccaggggac tttccactca gaacaggcca
     9421 ttgcatacgg cactaaaatg gttggcggcg taaccccagg taaaggcggc accacccacc
     9481 tcggcctgcc agtgttcaac accgtgcgtg aagctgttgc tgctactggc gctaccgctt
     9541 ctgttatcta cgtaccagca ccgttctgca aagactccat tctggaagct attgatgcag
     9601 gcatcaaact gattatcacc atcactgaag gcatcccgac gctggatatg ctgaccgtga
     9661 aagtgaaact ggatgaagca ggcgttcgta tgatcggccc gaactgcccg ggcgttatca
     9721 ctccgggtga atgcaaaatc ggtatccagc cgggtcacat tcacaaaccg ggtaaagtgg
     9781 gtatcgtttc ccgttccggt acactgacct atgaagcggt taaacagacc acggattacg
     9841 gtttcggtca gtcgacctgt gtcggtatcg gcggtgaccc gatccctggc tctaacttta
     9901 tcgacatcct cgaaatgttc gaaaaagatc cgcagaccga agcgatcgtg atgatcggtg
     9961 agatcggcgg tagcgctgaa gaagaagcgg ctgcgtacat caaagagcac gttaccaagc
    10021 ctgttgtggg ttacatcgct ggtgtgactg cgccgaaagg caaacgtatg ggccacgcgg
    10081 gtgccatcat tgccggtggt aaagggactg cggatgagaa attcgctgct ctggaagccg
    10141 caggcgtgaa aaccgttcgc agcctggcgg atatcggtga agcactgaaa actgttctga
    10201 aataaatatc tgtaataaga aatagccctc gccgcttccc tctacaggaa tggcgaaggg
    10261 ctgtcggttt cgacatggtt ggccatcgta tgatggcctt ttttatgcct taaatgttaa
    10321 ccacggtcga aatctgaccg ctggcttgac tgcatggcca cagccactgt ggattgccgc
    10381 aaccgaccgc aagccacgaa taagaacgac ggcgaatttc gaatcgttac ctgatgcact
    10441 ggcttcccag cgtttgaccg tagcgttaag taaatcaaca aaagcgatgt tcaccgctta
    10501 tcgtcaagtc gtgccatttg tcagccagga tcgggtcttt accccggata ttgcagcatc
    10561 gcaaaaattc ttagagggat atgctataaa tcaaaaataa aatctttgtt gattg
//
LOCUS       DAYPHN010000159        10534 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000159 DAYPHN010000000
VERSION     DAYPHN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.159"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            48..410
                     /locus_tag="ACG0BW_003923"
     CDS             48..410
                     /locus_tag="ACG0BW_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260824.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKNMNKGSDE"
     gene            complement(595..1053)
                     /gene="ybbJ"
                     /locus_tag="ACG0BW_003924"
     CDS             complement(595..1053)
                     /gene="ybbJ"
                     /locus_tag="ACG0BW_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFK7260825.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIV"
     gene            complement(1050..1967)
                     /gene="qmcA"
                     /locus_tag="ACG0BW_003925"
     CDS             complement(1050..1967)
                     /gene="qmcA"
                     /locus_tag="ACG0BW_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFK7260826.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2113..2790
                     /gene="fetA"
                     /locus_tag="ACG0BW_003926"
     CDS             2113..2790
                     /gene="fetA"
                     /locus_tag="ACG0BW_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFK7260827.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2777..3556
                     /gene="fetB"
                     /locus_tag="ACG0BW_003927"
     CDS             2777..3556
                     /gene="fetB"
                     /locus_tag="ACG0BW_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFK7260828.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3619..4473)
                     /gene="cnoX"
                     /locus_tag="ACG0BW_003928"
     CDS             complement(3619..4473)
                     /gene="cnoX"
                     /locus_tag="ACG0BW_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFK7260829.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4534..5343)
                     /gene="ybbO"
                     /locus_tag="ACG0BW_003929"
     CDS             complement(4534..5343)
                     /gene="ybbO"
                     /locus_tag="ACG0BW_003929"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFK7260830.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGVKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5333..5956)
                     /gene="tesA"
                     /locus_tag="ACG0BW_003930"
     CDS             complement(5333..5956)
                     /gene="tesA"
                     /locus_tag="ACG0BW_003930"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFK7260831.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQNKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5927..6613
                     /gene="ybbA"
                     /locus_tag="ACG0BW_003931"
     CDS             5927..6613
                     /gene="ybbA"
                     /locus_tag="ACG0BW_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFK7260832.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6610..9024
                     /gene="ybbP"
                     /locus_tag="ACG0BW_003932"
     CDS             6610..9024
                     /gene="ybbP"
                     /locus_tag="ACG0BW_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFK7260833.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(9242..9319)
                     /gene="dinQ"
                     /locus_tag="ACG0BW_003933"
     CDS             complement(9242..9319)
                     /gene="dinQ"
                     /locus_tag="ACG0BW_003933"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFK7260834.1"
                     /translation="MWREAVLIVLKALIALLELIRQFIE"
     gene            complement(9629..>10534)
                     /gene="mnmH"
                     /locus_tag="ACG0BW_003934"
     CDS             complement(9629..>10534)
                     /gene="mnmH"
                     /locus_tag="ACG0BW_003934"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFK7260835.1"
                     /translation="LALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQRSHIV
                     QRWLHEAGIDYPLVDGGYKALRQTAIQATIELSQKPIVLIGGCTGSGKTLLVQQQPNG
                     VDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQELRLWVLEDESRMIGS
                     NHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEY
                     LHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRY
                     QLEKKAEKVVFRGEWAEVAEWVKAQ"
BASE COUNT         2417 a         2767 c         2834 g         2516 t
ORIGIN      
        1 gataaatctc aatttttaag aggtttacgc caggcaggaa aatttaaatg aatgaatttg
       61 agaagatttt taatgaaatg aatctggacc gggcattgct accgatcctt tttcgttcga
      121 accggtcaac agtgtggaaa tatttaagtg gcgatagtac ggctcctgcc tcagctatgt
      181 cactgattat gctgttgcag ttaatccaga aacgaaaccc cgatctgtta gccgaatggt
      241 taacactttc tgatttcacc attccgccag aagtttacct ggaccagcct gattactgga
      301 aagggtgggt gtatacgcaa cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac
      361 attatccgga tgaagaccaa aaaaacatga ataaaggtag tgacgagtaa tttgccaatg
      421 ccagttaata tagcggaggc ggcctacgat tcgggcgcag acttccaggc atgataagac
      481 gcgcccgcgc cgcatcaggc atcagcgcat aaccgccgga tgcggcgtaa acgccttatc
      541 cggcctacaa ttcgggcata ttttgtaggc atgaaaagac gcgtcagcgt cgcatcagac
      601 gatgaccgca cggatgatca gcgttatccc ttctatcgca atgacttcaa catgcgtacc
      661 tgcaccgaga tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc gcatatgacc
      721 tcgcccgttg accagcggag attccagcac aaaacgtcgg ccaataagct gctgcccgcg
      781 ctggtttaaa tgactgtcgc tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca
      841 ccacagccag gcggcgagca gcgtcaggac ggcaaacatc accccttgcc actcccaacc
      901 cagcggcacc agccagacca ccagaccggt aatcaccgct gccacgccgc tccacaacaa
      961 ataaccattt ccgcccagca tctcggctgc cagcagcaac ccgccgagac tgagccagaa
     1021 aatatgtgga tgaacgacca ttaactccat catggctgag tccgtttgtt ggcgctgtct
     1081 ttcaccagct cggcaatccc ggcaatcgac cccatcaggc tgctggcctc taatggcatc
     1141 atcactactt tgctgttact ggaggaaccg atctgctgta acgcttcggt gtatttctgc
     1201 gctacgaagt agttcaccgc ctgaatatca ccggaggcga tggcttcaga caccattttg
     1261 gtggcgcggg cttctgcttc agcggaacgt tcacgagctt cagcctgtaa aaacgccgac
     1321 tgacgttcgc cttccgcttt caggatttgc gactgttttt caccttcggc tttgaggatt
     1381 tccgcctgac ggatcccttc cgcttcgagg atgtaagcgc gtttggtacg ttccgctttc
     1441 atctgcgcgt tcattgaaga gataagctct gccggtgggc gcacgtcgcg aatttcaata
     1501 cgggtgactt taatccccca cgggttggtg gcttcatcga caatacgcag caggcgtgag
     1561 ttgatgctgt cgcgctgaga gagcatttcg tcaagttcca ttgaacccaa cacggtacgg
     1621 atgttagtca tggtcaggtt gatgatcgcc agctccagat tgctgacttc ataggcagcg
     1681 cgtggcgcgt caatcacctg aataaagcac acggcgtcga tggtaacgtt ggcgttatct
     1741 ttcgagataa cttcctggga agggatatcg agcacttgct ccatcatatt gatcttgcga
     1801 ccaatgcgat ccataaacgg caccaccaga ctgagccccg gctgtaacgt tttggtatag
     1861 cgaccaaaac gttctactgt ccactgatag ccctgcggca cgattttgac gcccgcgccg
     1921 acaatgacca gcgcgacaaa aatgagaatc gggataaaga taagcatcgg aaaaacctcc
     1981 tgttgtaccg tccataatca gcaaaattgc tgcttgatta aacaaattat acctgattac
     2041 tgaaagagag ttccccctta ttcctgcgaa ggataaactg tttttagtaa aaatcagaaa
     2101 aagggaacag cgatgcagga aaatagtcct ttgcttcagc tacaaaacgt aggatatctg
     2161 gcgggtgatg cgaagattct taataacatc aatttttcgc tgcgtgctgg cgaatttaag
     2221 ttaattaccg gtccttctgg ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg
     2281 atcagcccaa ccagcggaac gttactgttt gaaggtgagg atgtcagcac actaaagcca
     2341 gaaatctacc gccagcaagt gtcttactgc gcccagacgc cgacgctgtt tggcgatacg
     2401 gtatacgata atctgatctt tccctggcag atccgtaacc agcagcctga cccggccatt
     2461 tttctcgatt ttcttgaacg tttcgctttg ccggacagca ttttgacgaa gaatatcgcc
     2521 gagctatctg gtggtgaaaa acaacgcatc tcattgattc gtaacctgca atttatgccg
     2581 aaggttttat tgctggatga aatcaccagt gcgctggatg aaagtaataa acataacgtt
     2641 aatgagatga tccatcgcta tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac
     2701 gataaagacg aaattaatca tgcggataaa gtgattacac tgcaaccgca tgccggagaa
     2761 atgcaggaag cacgctatga actcgcataa tattactaac gaatcattgg cactggcatt
     2821 aatgctggtg gtggtggcaa tcttaattag ccataaagaa aaactggcgc tggagaaaga
     2881 tattctctgg agcgtcgggc gcgcgataat tcagctgatt attgtcggct atgtgctgaa
     2941 gtatattttc agcgtggatg atgccagcct gacattattg atggtgttat ttatctgctt
     3001 taatgcggcg tggaacgcgc aaaaacgcag taaatatatt gctaaagctt ttatctcatc
     3061 gtttattgcc attacggtcg gggcgggaat taccctggcg gtgctgattc tctcagggtc
     3121 gattgaattt atcccgatgc aggtgatccc tatcgccggg atgatagccg gtaacgccat
     3181 ggtagcggtg gggttgtgtt acaacaattt agggcaacgg gtgattagcg aacagcaaca
     3241 gattcaggag aaactgagtc ttggcgcgac gccgaaacag gcttcagcga tattgattcg
     3301 cgacagtatt cgcgctgctt taattccgac ggtcgattca gcaaaaacgg taggcttagt
     3361 gagtttacca ggaatgatgt ccgggctgat atttgccggg attgatccgg tgaaggcgat
     3421 taaatatcag attatggtga cctttatgct gctctcaacc gccagcctgt cgaccattat
     3481 tgcctgctat ttaacctatc gtaagtttta caattcgcgc caccagttgg tggtgacgca
     3541 attgaagaag aaatgatgca atgccggatg cgcagcatcg catccggcgt tgtggttcat
     3601 gtgccgatca acggaaactc aatacaacaa cgcatataac tggcggcgat acttcgacgc
     3661 cagcgcatca cccgtaccca gcgcagcgag gatctcctgg aacgttttac gcgtctgacc
     3721 gtctgcggcg gtgagatctt tacgcagatg cccgaacagc aactccagcg cctcttcatt
     3781 gcgcccaacc tgatgcagtt gcagcgccag ttgcgtcgcc agtgcggcat cttctggatt
     3841 ttccgccacc tgctgttgca actgttgaat ttccggcgta tcagccgcct gcttcagcag
     3901 ttcgatttgc gccaccagcc cctggtagcg ggtgtcctga tcctgtaacg gaatggtttt
     3961 cagcacggct tcagcatctt cagaacggtt cagcgcaatc agcgtttctg ccagcagcag
     4021 gccgatctcc ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg gcagggcgtc
     4081 ggtgtaattg ccttcttgca tcagttgcat cgcctgctgt gctttcagtt cttcttcgcg
     4141 cggcagcact ttatccagca gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc
     4201 gtctaccggt tgcccgttct ggaacagata cacggtcgga atcgcgcgca gaccaaactg
     4261 ggcggcaatc atctgctccg cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta
     4321 ctgcgccgcg aggctttcca gaattggggt taactgcaaa cagtgctggc tacgttcaga
     4381 ccaaaaatag aacagcaccg gagtggtcat cgactgttca agaacctgtt gcaggttaga
     4441 ttcgttaatg ttgacaatat tttctacgga catggagtcg ctctctgttg tcgatatttt
     4501 ctttgacatg ggggcttaag cgcgcgcttc aactcacccc tgcaatattt tgtccatcac
     4561 gcgccccggc agcaggcgct taagcaccat tactgcccag gtcaccagcg ttaccggata
     4621 gcgcatcttc ggtttctcgc taataaaagc atggcgtact ttgtcgacca ccgcttccgg
     4681 tcccaacgta aagcgggcgg cgatgccagg attttcgact ggtttatcac tttgcgtctg
     4741 gttgacgttg tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc taactttaac
     4801 tccgctgtgg cgcagctcca tgcgcagcgc atccgaccac gcctccagcg catatttact
     4861 ggctgcgtaa gcgccgcgac ccggcgtgga gattaatccc atcaccgatg aggtcatcac
     4921 aatacgccct tcaccgtgcg gtaacatcgc gggtaacagg cgcatggtga gctggtgagc
     4981 gccgaaaaag ttggcagaaa actgctgttc catctgcgca cggctgatgg tggaaagggg
     5041 gccatacatg ccgaatccgg cattgttaaa gatcccatac agacaattat cggtcagggc
     5101 gatcacttcg tcggctgcgc gatcaacact ttctggtgaa tccagatcga tcaacacgcc
     5161 ggtaaatccc atgctgttca tgcgctcaac atcatccggt ttccggcaac ctgccagcac
     5221 atgaaaaccc tggcgtttta attcgagcgc gctttccagg ccaattccac tggaacatcc
     5281 ggtaattaag accgattttt gcataacttt acctgtcagg atctccgttg ctttatgagt
     5341 catgatttac taaaggctgc aactgcttcg ccatccagtc ggcaataaac ggctgggcgt
     5401 cgcggttggg atgaataccg tcatcctgca tccattgtgg cttgaggtag acctcttcca
     5461 taaaaaaggg cagcagcgga acatcaaact ctttggcgag tttggggtaa atggcgctaa
     5521 aggcttcatt ataacggcga ccatagtttg caggcagacg tatttgcatt aacaatggtt
     5581 cagcgttggc ggctttgaca tcctgcaaaa tctggcgcag cgtctgctcg gtttgttgtg
     5641 gctgaaaacc acgcaaaccg tcattgccgc ccagttctac cagcacccag cgcggctgat
     5701 gctgtttcag cagagccgga aggcgcgcca gcccttgttg cgaggtgtcg ccgctgatgc
     5761 tggcattgac taccgacgtt ttattctgcc acttatcatt caacaaggca ggccaggccg
     5821 cgctggcaga cattcgatac ccggcgctca ggctatcacc cagaatcaat aacgtgtccg
     5881 ctgcggcggc acggaaggtt aacaggacca ggaacaggaa gggcaaatgc cagcggaaaa
     5941 cattgttgaa gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg aactctccat
     6001 cctcaccgga gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg tgggcgagtc
     6061 gggatcgggt aagtcaacct tgctggcgat cctcgccggg ctggatgacg gcagcagtgg
     6121 cgaagtgagt ctggtgggac aaccgctaca taatatggac gaagaagcgc gggcaaagtt
     6181 gcgcgcgaag cacgtcggct ttgtttttca gtcatttatg ttaattccta cccttaacgc
     6241 gctggaaaac gtcgagcttc cggcgctgct gcgcggtgag agtagcgcgg aaagtcgtaa
     6301 cggagcgaaa gcgttgctcg aacagttagg gctgggtaaa cgtctggatc atcttccggc
     6361 acagctttcc ggcggtgaac agcaacgagt ggcgctggca cgagccttta acggtcgacc
     6421 tgatgtgctg tttgccgacg aacccaccgg caaccttgac cgccagaccg gcgataaaat
     6481 tgccgacctg ctgttttccc tcaaccgtga acatggcacc acgttgatta tggtgaccca
     6541 cgacctgcaa ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg ggcagttgca
     6601 ggaggaagca tgattgcacg ttggttctgg cgcgaatggc gttcgccgtc gctattaatt
     6661 gtctggctgg cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat
     6721 cgcatggaga agggcttaag ccaacaaagc cgggagttta tggcgggcga tcgggcgttg
     6781 cgcagttcgc gcgaagtgcc gcaagcatgg ctggaggaag cgcaaaagcg cggcctgaaa
     6841 gtcggcaagc agctgacttt cgccaccatg acatttgcag gcgacacacc gcagctggcg
     6901 aacgttaaag cggtggatga tatctacccg atgtatggcg atctgcaaac caatccccct
     6961 ggcctgaaac cgcaggcggg cagcgtattg ctggccccac gcctgatggc actgcttaac
     7021 ctaaaaacgg gcgacaccat tgacgtgggc gatgccacct tgcggattgc cggagaagtg
     7081 attcaggaac cggattccgg ttttaacccc ttccagatgg ctccgcgtct gatgatgaat
     7141 ctggcggatg tcgataaaac cggagccgtg cagccgggga gtcgggtcac ctggcgctat
     7201 aaattcggcg gcaacgagaa ccagctcgac agctatgaga aatggttgtt acctcagctt
     7261 aaacccgaac aacgctggta cggtctggaa caggacgaag gcgcgctggg gcgctcgatg
     7321 gaacgctcgc aacagttcct gctgctttcg gcgcttctga ccttgctgct ggcagtggca
     7381 gcggtggcgg tggcgatgaa tcattactgt cgcagtcgct acgatcttgt ggcgatcctc
     7441 aaaacgctgg gggcagggcg agcgcaactg cgtaagctaa tcgtcggtca gtggttgatg
     7501 gtgctgacgc tttcagccgt taccggcggg gcgataggcc tgttgttcga aaacgtgttg
     7561 atggtgctgc tcaagcccgt tttgcctgct gcactaccgc cagccagcct ctggccgtgg
     7621 ttgtgggcgc tgggcaccat gacggtcatc tcgctgctgg tggggctacg accgtaccgc
     7681 ttgttgctgg caacccagcc tttacgcgta ttgcgtaatg atgtggtcgc gaacgtctgg
     7741 ccgctcaagt tttatctgcc gattgtcagt gtggtggttg tgctgctgct cgccggatta
     7801 atgggcggca gtatgctgct ctgggcggtg ctggcgggcg cggtagtact ggctttgctg
     7861 tgcggtgtgc tgggctggat gctgctgaat gtacttcgcc gcatgacgct gaaatcgcta
     7921 cctctgcgcc tggcggttag ccgcctgtta cgtcagccgt ggtcaacgtt aagccagctt
     7981 tcggcatttt cgctctcctt tatgctgctg gcgctgttgc tggtattgcg tggcgatctg
     8041 ctcgaccgct ggcaacagca gctaccgcca gaaagcccga actacttttt aatcaacatc
     8101 gccacagaac aggtagaacc gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa
     8161 tcattttatc cggtggtgcg ggcgcgactg acggcgatta acgacaagcc gacagaaggc
     8221 aatgaagatg aggcgcttaa ccgcgaactc aatcttacct ggcaaaatac gcggcccgat
     8281 cataatccga ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc aatggaagag
     8341 gggctggcga aacgcttaaa cgttgccctc ggcgataccg tgacttttat gggcgatacc
     8401 caggagttcc gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag tctgcggcct
     8461 aatttctatt ttattttccc tgaaggggca ttagacggac aaccgcagag ttggcttacc
     8521 agtttccgct gggagaatgg caacggcatg ttgacgcaac tcaaccgcca gttccctacc
     8581 attagcctgt tagacattgg cgcgatttta aaacaggtcg gtcaggtgct ggagcaggta
     8641 agtcgggcgc tggaagtgat ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg
     8701 gcacaggtgc aggtgggaat gcgtcagcgt catcaggagc tggtggtgtg gcgcacactc
     8761 ggtgcgggca aaaaactgct gcgcacgaca ttgtggtgtg agttcgccat gctcgggttt
     8821 gtatccggcc tggtggccgc aattggtgcg gaaacggcac tggcggtact gcaatcgaaa
     8881 gtgtttgatt tcccgtggga gccagactgg cgattgtgga ttgttctgcc gtgcagcggt
     8941 gcgctgctgc tgtcgctttg cggcggctgg ctgggtgcgc gactggttaa gggtaaggcg
     9001 ctattcaggc agtttgtggg atgatgacag gcgttagatt gcctgatgcg ctacgcttat
     9061 caggcctacg aggatcgtgc aatatattga ttttgcaaga ttgtgtaggc cggataaggc
     9121 gtttacgccg catctggcat gaacagtgtg cattttggca ataaatatga aaccaactgt
     9181 catgtcggtt tttttaaagg ccgaaggtgg taaaccttcg gccttagcta cgtttcctca
     9241 gttattcaat aaactgacgg atcagttcca gcagcgcgat tagcgcctta agaacgatga
     9301 gaactgcctc tctccaaatt agagcaccag aatcagcacc acctgtatcc tgaccaggac
     9361 ttaaaagtac ttttccgcac tgcggtaatc ccttgaggca gtatcactac tagcgtcggg
     9421 attgtatcgc caacatagct gtttgaatat gtaggcatcc tgacttataa ttcatttgct
     9481 agatgaggac tgtttcattt ctgcccggaa ccaccaagtc ttgggcggaa aaaataaaaa
     9541 acccgactgt catgtcggtt tttttatatc tgtattctgg tgcaaccatt ctgttctcac
     9601 gacaatcgcc gtttcagaat accgcccatc actgcgcctt aacccattcc gccacttccg
     9661 cccactcacc gcgaaagaca actttttcag cttttttctc aagctgatag cgatacatcg
     9721 ggtcgtaata ttcttcaagt aacggcacca gccaggccag atgaccgtcg gtgctgccgg
     9781 tggtgagttg cgttgtcagc gccgtatcca gctgggcagc gagttcgtta tagcgctgta
     9841 accccagccg acgcttaatc gccgaaagtc cgtgatgcag gtattcgcaa tactcctgcc
     9901 agccctgttc gtcgccgtac gcgtgggtaa aatcatgatg catacgcaag aaatactctt
     9961 cgttcaggcg ttcaagacgg atctcaaacg gatcttctac caccgcaatc gccgcctgag
    10021 tcattcgctc gcgcagacat tccggcaggt gatttgaacc gatcatccgg ctttcatctt
    10081 ccagcaccca caggcgcagc tcctgacggg cgtcggtttt tagcatttcg gcagccagca
    10141 gattttcaaa actcgcctgg ctaagttgcg gttgcaacgt gcgaccaaac gccgaaccgc
    10201 gatggcgcgc cagcccttcc agatcaacac cgttcggctg ttgctgcact aacagcgttt
    10261 taccgctgcc agtacaaccg ccaatcagca ctatcggttt ttgtgacagt tcaatggtcg
    10321 cctgaatcgc ggtctggcgc agtgccttat aaccgccgtc taccagcgga taatcaatcc
    10381 ccgcttcatg caaccagcgt tgcacaatat gtgaacgttg accgccgcgg gcgcagcaga
    10441 gaataccttg cggattttgc aggcacgctg cccgccaggc gtccatgcgc tgctgacgaa
    10501 tttcacccgc caccagttta tgccccagcg ccag
//
LOCUS       DAYPHN010000160        10502 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000160 DAYPHN010000000
VERSION     DAYPHN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.160"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            89..1456
                     /gene="dcuC"
                     /locus_tag="ACG0BW_003935"
     CDS             89..1456
                     /gene="dcuC"
                     /locus_tag="ACG0BW_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFK7260836.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1500..1997)
                     /gene="sspB"
                     /locus_tag="ACG0BW_003936"
     CDS             complement(1500..1997)
                     /gene="sspB"
                     /locus_tag="ACG0BW_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFK7260837.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2003..2641)
                     /gene="sspA"
                     /locus_tag="ACG0BW_003937"
     CDS             complement(2003..2641)
                     /gene="sspA"
                     /locus_tag="ACG0BW_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFK7260838.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3036..3428)
                     /gene="rpsI"
                     /locus_tag="ACG0BW_003938"
     CDS             complement(3036..3428)
                     /gene="rpsI"
                     /locus_tag="ACG0BW_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFK7260839.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3444..3872)
                     /gene="rplM"
                     /locus_tag="ACG0BW_003939"
     CDS             complement(3444..3872)
                     /gene="rplM"
                     /locus_tag="ACG0BW_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFK7260840.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4091..5218)
                     /gene="zapE"
                     /locus_tag="ACG0BW_003940"
     CDS             complement(4091..5218)
                     /gene="zapE"
                     /locus_tag="ACG0BW_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFK7260841.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5412..5810
                     /gene="zapG"
                     /locus_tag="ACG0BW_003941"
     CDS             5412..5810
                     /gene="zapG"
                     /locus_tag="ACG0BW_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFK7260842.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5964..7331
                     /gene="degQ"
                     /locus_tag="ACG0BW_003942"
     CDS             5964..7331
                     /gene="degQ"
                     /locus_tag="ACG0BW_003942"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFK7260843.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7421..8488
                     /gene="degS"
                     /locus_tag="ACG0BW_003943"
     CDS             7421..8488
                     /gene="degS"
                     /locus_tag="ACG0BW_003943"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFK7260844.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8551..9489)
                     /gene="mdh"
                     /locus_tag="ACG0BW_003944"
     CDS             complement(8551..9489)
                     /gene="mdh"
                     /locus_tag="ACG0BW_003944"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFK7260845.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGANVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEKNALEGMLDTLKKDIALGEEFVNK"
     gene            9924..10394
                     /gene="argR"
                     /locus_tag="ACG0BW_003945"
     CDS             9924..10394
                     /gene="argR"
                     /locus_tag="ACG0BW_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFK7260846.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2556 a         2661 c         2641 g         2644 t
ORIGIN      
        1 ataaataacc ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt atgggattga
       61 ccgttttctt ttgacacgga gttcaacaat gttcggcata attatatctg tcatcgtatt
      121 aattacgatg ggctatttga tcctgaaaaa ctacaaacct caggtggtgc tggctgccgc
      181 aggtatcttc ctgatgatgt gcggtgtctg gttagggttc ggtggtgtgc tcgatcccgc
      241 caaaagcagc ggctacttga tcgtcgatat ttataatgaa atcctgcgca tgctgtccaa
      301 ccgcattgct ggattggggc tgtcgattat ggcagtgggc ggttatgccc gctatatgga
      361 gcgcataggg gcaagtcgcg cgatggtgag cttgttaagc cgcccgttaa aactcattcg
      421 ctcgccgtat attattctgt cggcaactta cgtcatcggc caaatcatgg cgcagtttat
      481 taccagcgcc tccggtctgg gtatgttgct gatggtcacc ttatttccga cgctggtgag
      541 tctgggagta agtcgtctct ctgcggtggc ggttatcgca accaccatgt ccattgagtg
      601 ggggattctg gaaacgaact ccatttttgc tgcccaggtc gcaggaatga aaattgccac
      661 ttacttcttc cactaccagc ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc
      721 ccactttttc gtgcaacgcg ctttcgacaa aaaagataaa aatatcaatc acgaacaggc
      781 agagcaaaaa gctctcgata atgtcccgcc gctctattac gccattttac cggtgatgcc
      841 gttaatcctg atgctcggct cgctgttcct tgcccacgtc gggctgatgc agtcagaact
      901 gcatctggtg gtggtgatgt tactgagttt gacggtgacg atgtttgttg agttcttccg
      961 caagcataac ttgcgcgaaa caatggatga tgtgcaggcg ttttttgacg gcatgggtac
     1021 gcagtttgcc aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga aaggcttaac
     1081 gacgattggc actgtcgatg cggttatcag gggtgcggag cattctggtc tgggcggtat
     1141 tggcgtgatg attattatgg cgctggtcat tgccatttgt gccattgtga tgggctcagg
     1201 caatgcgccg tttatgtcat ttgccagtct tattccgaat atcgcagccg gactacatgt
     1261 gccagcggtt gtaatgatta tgccgatgca ttttgccacg acgctagcgc gcgctgtttc
     1321 gccgattact gcggtggtgg tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt
     1381 agtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga ttgccacaat
     1441 cacgctattt tattaagtca ttaaaaagac aaaacaggcc gccagggcct gttttagtat
     1501 tacttcacaa cgcgtaatgc tggtcgacca ccacgtggtg gctgcggtgg ttcatcgtca
     1561 ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat aacggtttcg
     1621 ttgtctgccg atgcctcttc atcattcatg atgctggcat cttcatcgta ggcagcttca
     1681 ggttcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac ggcagccagc
     1741 ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac ctcatcattc
     1801 gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg cccgtcacgc
     1861 gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag gtgcggcgtg
     1921 agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg acgtggtgtt
     1981 agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt tcagcttcag
     2041 ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct ttcagctctt
     2101 tcgcacccgg gccgctgaac tcgataccca gttgcggcag acgccacagc agcggagcaa
     2161 ggtagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga ccgaagaccg
     2221 gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct tcagaagctg
     2281 aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga tgcatgtaca
     2341 gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc gggaaacgct
     2401 catccagata ttccataatg atgcgagatt cccacagggt cagctcacga tccaccaggg
     2461 tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg tccttttcca
     2521 cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc tgatggctat
     2581 agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca gcgacagcca
     2641 tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt ggccagtcag
     2701 aagttgtgtt acccagtaag gaacgactct ctttgttcga aaatcaaaca aaaaatgagc
     2761 aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc gacctttgtg
     2821 gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat agttgactta
     2881 gtcccttatt ggcgatgtgg tttttgtttt gcctgtctgt cagacggcag caaaaagcaa
     2941 ctttccattt tttacgctga ttcagaattt agccataaaa aaacccgccg aagcgggttt
     3001 tttcgcaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa ctgcggacga
     3061 cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc acgagtaacg
     3121 aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag agcgcgggtg
     3181 ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac ggtgatgtac
     3241 aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac catgcgggca
     3301 gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt accgttgccc
     3361 ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta gtattgattt
     3421 tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg tgccgcgtgg
     3481 ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc cagcgggcct
     3541 tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg agcaatcatc
     3601 tcttcaaagg tcgcttgttt gataccaccg atgtggccgg tgtggtgata gtacactttg
     3661 tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat gatgtaatca
     3721 ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg acgagccagt
     3781 tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca gtcgcgtttt
     3841 acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa atagttacac
     3901 gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt ccggcaaacc
     3961 taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa ctttcttgta
     4021 acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt
     4081 gtggcaggtt ttaacccgct aaatgctcgc gcttcagata ctcttcgctt tgcatctctt
     4141 gcagacgtga caggcaacgc tggaactcaa atttcagccg ctcgccctga taaatgtcat
     4201 atagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca
     4261 gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggc acatcaaaca
     4321 acatgaccgt gtgaaagagg cgtgagagcg caatatagtc atgctgactg cgggcgtcga
     4381 cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt gtcgccaatg
     4441 gccgatggtt gatttctaac gtcggtgaat tttctcgttt cgcccccgcc agcgccaacc
     4501 atagtttatc catttgcgcc cgggtttcat cgttgagggg cgaaagccac agatgcgcct
     4561 gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca tcacaatgct
     4621 gtttaatggc atcgattgca ggcaaaaaac gcgcacgttg taggccattt cgataaagtt
     4681 cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac agggctttca
     4741 tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg tcaaaacaga
     4801 gcacgtcagt ttctgcttta aagcgatcgg caataatttc cagcggatcg ctttggcctt
     4861 gtaattcggt cagctcttcg tgcacccgca gcataaaacg gtgaaagtgc agacgctgtt
     4921 tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta
     4981 caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg cgtttacccc
     5041 acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggaggc gtgctattga
     5101 tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt
     5161 gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga
     5221 tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc
     5281 cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa cggttatagt
     5341 ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac aaaagacaac
     5401 gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg
     5461 gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg
     5521 aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg
     5581 cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca
     5641 tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac ccgttccgta
     5701 atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact
     5761 attctgaagg cgcgtccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttc
     5821 gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc
     5881 tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc
     5941 attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt gcattagcgt
     6001 taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt tgcgtcgatt ccaggccagg
     6061 ttgccgatca ggcccccctc cccagtctgg ccccaatgct ggaaaaagtg cttccggcag
     6121 tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca
     6181 aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa ggtttaggct
     6241 ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat gtgattaatc
     6301 aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg
     6361 gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc aaattaacgc
     6421 aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg gcggtcggta
     6481 acccatttgg ccttgggcaa accgccacct ctggcattgt gtcagcatta ggccgcagtg
     6541 ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt aaccgcggta
     6601 actccggcgg tgcactgtta aatcttaacg gtgagttaat tggcatcaac acggcaatcc
     6661 ttgcgcctgg tggcgggagc gtcgggattg gatttgccat ccccagtaat atggcgcgaa
     6721 cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtctgtta ggcatcaaag
     6781 gcacagagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt
     6841 ttgtcagcga agtgttgcca ggttctggct cggcaaaagc gggcgtcaaa gcgggcgata
     6901 ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg
     6961 caaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc aaaccactgg
     7021 aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa atgatcacgc
     7081 cagcgcttga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc aaaggtatta
     7141 agatcgatga ggttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg
     7201 tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg
     7261 cggcaaaacc ggccattatc gccctgcaaa ttgtacgcgg caatgaaagc atctatctgc
     7321 tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg ttaactcgtg
     7381 gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga agctcttacg
     7441 ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg
     7501 cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc ctgccagcta
     7561 taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc gaggtttgaa
     7621 caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa tcatggatca
     7681 acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga tcatcgtcgc
     7741 cttacaggat ggacgtgtat ttgaagcatt gctggtggga tctgactctc taaccgatct
     7801 ggcggtactt aaaattaatg ccactggcgg tttacctact attccaatca atgcacgtcg
     7861 cgtaccgcac attggcgacg tggtactggc gatcggtaac ccgtacaacc tcgggcagac
     7921 cattacccag gggattatta gtgccacggg tcgaattggt ctgaacccaa ccgggcggca
     7981 aaacttcctg caaactgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa
     8041 ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga
     8101 aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa ttatggataa
     8161 actgatccgc gatggtcgcg tgatccgcgg ctacattggt atcggcggac gtgagatcgc
     8221 accactgcac gcgcagggcg gtggtataga tcaactgcaa gggatcgtgg ttaatgaagt
     8281 gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aatgatttga ttatttcggt
     8341 ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcgg aaattcgccc
     8401 tggttcggtg atcccggttg tcgtgatgcg tgatgataag cagttaacgc tgcaggtcac
     8461 cattcaggaa tatccggcaa ccaattaagt cgtgcgctca aaacaaaaaa ccggagtctg
     8521 cgctccggtt ttttattatc cgctaatcaa ttacttatta acgaactctt cgcccagagc
     8581 gatatctttc ttcagcgtat ccagcatacc ttccagcgcg ttcttttcaa atgcgctcag
     8641 agtgccgata gatttacgct cttccacgcc gtttttaccc agcagcagcg gttgagagaa
     8701 gaaacgggcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc
     8761 ctgcagtgcg cgtaccagag acagaccaaa acgtgcagct gcctggccca tagacagggt
     8821 tgcagacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt
     8881 ggtcagatca gccacttcct gctcggtaaa gctaacgcca ggaacctgtg acagcagcgg
     8941 cagaatggta acaccagagt gaccgccaat aaccggcact tcaacttcgc ctggctgttt
     9001 gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa
     9061 cagtttgttt ttgtcataaa caccggcttt tttcagcact tcagcagcaa tcgcaactgt
     9121 ggtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg
     9181 ctgtaccagg tttttcacga tgccggcgtt aacgttaaac aggtcggaac gatccatacc
     9241 cggtttacgc gctacacctg cagagataag aacgacattt gcgccttcca gcgccggagt
     9301 cgcatcttca ccagaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac
     9361 agccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag
     9421 ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc
     9481 gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc
     9541 gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc
     9601 aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg taattaacag
     9661 tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta ccgttcagcc
     9721 aaaacaacat caatttgata acaattaatt tacttttaag cagaatttgc atgccgtgac
     9781 gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc
     9841 agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa taatgttgta
     9901 tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag
     9961 catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt
    10021 tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt
    10081 ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac
    10141 tgggtgtacc aaccacctcc agtccattga aaaatctggt gctggatatc gactacaacg
    10201 atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct cgcctgctgg
    10261 actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta
    10321 ctacccctgc taacggtttc accgtcaaag acctgtacga agcgatttta gagctgttcg
    10381 accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttatccc
    10441 tctcaacccc ctgctttcct ctgcgattaa tttaacgaat agtgcgtttt actgcgacat
    10501 gt
//
LOCUS       DAYPHN010000161        10482 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000161 DAYPHN010000000
VERSION     DAYPHN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.161"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            233..442
                     /gene="yibT"
                     /locus_tag="ACG0BW_003946"
     CDS             233..442
                     /gene="yibT"
                     /locus_tag="ACG0BW_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFK7260847.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(454..1041)
                     /gene="mtlR"
                     /locus_tag="ACG0BW_003947"
     CDS             complement(454..1041)
                     /gene="mtlR"
                     /locus_tag="ACG0BW_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFK7260848.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1041..2189)
                     /gene="mtlD"
                     /locus_tag="ACG0BW_003948"
     CDS             complement(1041..2189)
                     /gene="mtlD"
                     /locus_tag="ACG0BW_003948"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFK7260849.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(2418..4331)
                     /gene="mtlA"
                     /locus_tag="ACG0BW_003949"
     CDS             complement(2418..4331)
                     /gene="mtlA"
                     /locus_tag="ACG0BW_003949"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFK7260850.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4868..5230
                     /locus_tag="ACG0BW_003950"
     CDS             4868..5230
                     /locus_tag="ACG0BW_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFK7260851.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5233..6369
                     /locus_tag="ACG0BW_003951"
     CDS             5233..6369
                     /locus_tag="ACG0BW_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFK7260852.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            6440..7048
                     /locus_tag="ACG0BW_003952"
     CDS             6440..7048
                     /locus_tag="ACG0BW_003952"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFK7260853.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            7146..8537
                     /gene="selA"
                     /locus_tag="ACG0BW_003953"
     CDS             7146..8537
                     /gene="selA"
                     /locus_tag="ACG0BW_003953"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFK7260854.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDIPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATVRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            8534..10378
                     /gene="selB"
                     /locus_tag="ACG0BW_003954"
     CDS             8534..10378
                     /gene="selB"
                     /locus_tag="ACG0BW_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFK7260855.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQIHRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQVIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         2482 a         2674 c         2821 g         2505 t
ORIGIN      
        1 gtgctttgtt cacaactgat gacattgcaa tagttctata acgtaaacat taacatgctt
       61 aaaaactaag caatttattg attaattaag aaatttaaag aaaaaagata gttaatagaa
      121 acggatgatt attgaggatt gagcacgttt tccttaatgt catttatacc gggatctaaa
      181 ctaaaattgg cggccgcgca ttcccggcgc ggggtaatac ggagatatca tcatgggcaa
      241 attaggtgaa aacgttccgc ttcttatcga taaagccgta gatttcatgg catcgagcca
      301 ggcgttccgg gagtatctga aaaaactacc tccccgtaac gcgattccgt ccggaatacc
      361 tgatgaaagc gtgccgttat atctacaacg tctggagtat tatcgtcggc tttatcggcc
      421 gaagcaggta gaggggcagt aagcagacgt tgcttacttt tggaaggctt tttttaagct
      481 gattttggaa atcagctctg tcagtgaaag gaccattgtt gatcgcacag cctgttggta
      541 acgctgaatt tgcattgcat acaaactgga gtctgcaggt tcaaactgtg gcggcggtgg
      601 taacgccgcc acgcaatgca gttcaccaaa gggtccaagg atttcgtcgt cggtaaacgc
      661 gtactcgttg ccgtcgtgat ttagctcttc acgcaatgcc atcagtagct ccgcatcttc
      721 gtattcctgg cggttaatga cgcccaaccc gtaaatgagt tttaaacgca cagaaagatc
      781 gcccagcgga ccgtcgccgt cgagtaacgg ttctacagca tacttcaccg cgtaatcgtc
      841 tttgcggaat acctgaagca ccagaagatt taccgcctcg gtcaaaagct cgacggcggt
      901 gatcagaaag cttcgcacgg ttttgccagc attcagacgc tcaagcacac ggttttcaaa
      961 ggcctgggtt tgttccattg ttgcctgcat atctgacaat ctgttattcg ggcgcagggt
     1021 atcctgcgcc tggtccacca ttattgcatt gctttataag cggttaccgc ctcggataca
     1081 acctcgctgt tggcatcaag accggaaatc tgtgccagcg ccgcctgcgg acctttgtca
     1141 gcgatcagtg ctgccagttc ctgagcctgc ggatcatctt cactgcggaa gtgcattgca
     1201 gcggcaatac cttcaatcag gtttttatgt ggcagaccat attccagcgt tccgagcagt
     1261 ggcttgatca gacggtcgcc agcactcagt ttacgcagcg gctgacggcc tacgcgctct
     1321 acatcatctt tcagatacgg gttctcgaaa cgaccgagga ttttccggat gtacgccgca
     1381 tgtttgtctg cgtcaaagcc gtagcgcttg atcaatactg caccactttc ttccatcgca
     1441 ccttttacca ccgcgcggat tttctcgtcg agaatagcgt cacgaatggt ctgatgaccg
     1501 gccagttttc cgaggtacgc ggttatagca tgacccgtgt tcagggtgaa gagtttacgt
     1561 tcgacaaatg ccatcaggtt gtcagttaac tccatgcctg ggatgttcgg cagcgcgcct
     1621 ttgaactggg ttttatcaac aatccattcg ctgaatgttt ctaccgtcac ttccagcgga
     1681 tcgttggttg cagaagccga aggcggcacg atgcggtcaa cggcggaatc gacaaagcca
     1741 acgtgttctt ctacccacgc tttggcatct tccggcaggg cgttcattac atggcctttc
     1801 agctgcgtgg tgccgcgtac catgttttca caggcgatga tgttcagcgg ggattcatta
     1861 ccttgttctt tacgtttcac cagccctttg gcgattgccg gggcaatacg ttccagcaca
     1921 accggaccaa cggcggtagt gactaaatca acctgagcaa tcagatcaac gacatcatca
     1981 ccaatgctgc tgacggcatt gacgccggaa acggtatcta cctgctcggt ttcaccgacc
     2041 acatgaacct gatagctatg acgggcattc agggcatcaa gtaccacctg attgacatcg
     2101 gcaaacgtca gttggatacc cgcgtctgcc agcagtttac cgataaagcc acgaccgata
     2161 ttacctgcgc caaaatgtaa tgctttcata gtattaacct tcatcaatgt ttttacccga
     2221 gagggctggg gtgaggcttt tccctcaccc aaaccctctc catgtggaga ggggccgtgc
     2281 cggatgcggc gtaaacgcct tatccggcct acaagaacgt gcaaattcaa caaactgcgc
     2341 tttgttttgt aggcctgata agcgtagcgc atcaggcaat aagccccacc ttctccatgt
     2401 ggagaggtgg gattggatta cttacgacct gccagcagtt ccagcacttc atccacgctg
     2461 gtggtgtgtg ccagacgctc gatgacagac tcatcatcca gtgcattggt caggctggtg
     2521 ataacctgaa tgtgctcgtt gttacgggca gcaataccaa tcaccagacg ggcaatgtca
     2581 tcttcttctt caccgaagcg cacgccttcc gggtactggc agaacaccac gcccgttttc
     2641 agtacgcgat cttttgcttc aaccgtaccg tgtggcaccg cgatagactc acccagataa
     2701 gtcggggtca gtttttcacg atccagcatc gcctgaacgt attccggctc aacgtaaccg
     2761 cctttcacca gctgctcgcc agcaaaacga atcgcttctt ctttggttgc cgctttgcga
     2821 ccgaggaaga tgttctccgc gcccagtttg aacaggttag cactggaatc gtcaaagctg
     2881 tctttcagac tgtctttcac tttcacttcg ttttcagtat ggcgctgggc agcaaccaga
     2941 cgttcggtca ggctggtgta caggccgctg tcgaggaagt tggtcagcga aatatgctgt
     3001 gcctgcggaa cctggcgcat agcgcgttcg gtcaggtcac ggtgagtgat gaccaggtcc
     3061 acatctggcg gcaggttgtt gatcgcgctg ttagtgaccg aaatctgcga cagacctgca
     3121 tcctgaattt tcttacgcag cacacctgcg cccatcgcac tggaacccat accggcatca
     3181 caggcaacga tgattttacg tacgtggctc aggtcgttag tcacatcgcc cgcagacagc
     3241 ggagacgcgc ctttagactc agctttcatg tcctgcatac gacgagttgc tgcttcaata
     3301 tcatcttctt ctttcacttt gctggttttc agcagaatgg cagagacaac gaaggagaca
     3361 gccatcgccg cacacacacc cgcgatgtta gcgaagtatg caccttttgg tgtcatcgcc
     3421 agtacagcaa ggatagaacc cggagatgcc ggagaaacca gaccaccgcc caggatagtc
     3481 agcgtgaaca cgccagtcat accaccgagg ataacggcca gaatcagacg cggattcatc
     3541 agcacatacg ggaagtagat ttcgtggata ccgcccagga agtggatgat tgccgcaccg
     3601 cccgcagact gtttagcgct accacgccca aagaacatgt acgccagcag cacgcccata
     3661 cccggacctg ggttagcttc aatcaggaag aagatggatt tacccagttc atgggactgc
     3721 tgaataccca gcggcgagaa gataccgtgg ttaatggcgt tgttgaggaa caggattttc
     3781 gccggttcaa caaagataga cgccagcggc agcatgtcat ggacaaccat gaagttaacg
     3841 cccgcagcca gcattttaga cagagcttca acaatcgggc caatgccgag gaatgccaga
     3901 atagcgagga tcatcccgat gatgcctgcg gagaagttat tcaccagcat ctcaaaaccg
     3961 gatttgatct taccgtctac ccagcggtcg aagtgcttaa tgcaccagcc gcccagcgga
     4021 cctgcaatca tagaaccgag gaacatcggc atatctgcgc cgacgataac gcccatggtg
     4081 gtgatggcac cgactacacc gccacgttcg ccgcctacca gcttaccacc ggtataaccg
     4141 atcagcagcg gcaggagata agtgatcatc ggcccgacca gcttcgccag cgtttcgttc
     4201 ggtaaccacc ctgttggaat aaataacgcg gtgatgatac cccacgcgat aaacgcgccg
     4261 atatttggca tcaccatgtt gctgaggaaa cgaccaaagc tttgcacttt gatcttaata
     4321 tcggatgaca taaaaacacc ccttcttatg tttgctgtcg cgcaggctgg aagcccgagg
     4381 ttttttgtta atgtggcggc agaggtagcc ggaccttgta gatactgcga aatctggcac
     4441 tgaatcggtt aactgtccag tcgacggcct tttgtgtgat atcagtcaca taaatatagg
     4501 gggtagagag gtaattgacg tgatgctcat cacaaaacga ctgtgtaaaa aagcaacaag
     4561 gcggcgattt tcgctaaaaa tggcatttaa atgtgagtaa tgtcacattt ttgttttgtt
     4621 agtttgttga atctttgtga tctgaatcac aagatatttt cttacggcgt tcacctgatg
     4681 tgatctgcag catgttatgc cttcgtcata aaatcgttat gtaactaagc aactcatcac
     4741 ctctaaaaat tattctttgg tgttatggcg agaatcgcga acccgaatta ttgtcaaaat
     4801 atcagcctga ctcccttctg gctgccagcc aaaaaacaac accgataaac gtgaatccga
     4861 ggcagatatg tttctaaact atttcgcgtt gggagtgctg atcttcgtct ttctggtgat
     4921 tttttatgga atcatcgcga tacatgacat cccttatctg attgccaaaa agcgcaacca
     4981 tccccatgcc gacgctattc atacggcggg ctgggtgagc ctgtttactc tacatgttat
     5041 ctggccgttc ctgtggatct gggcgacgct ctatcaaccg gagcgcggct ggggcatgca
     5101 gtcacatgtt gcgtcgcaag agaaagcgac tgacccggaa atcgccgcac tttctgaccg
     5161 aatttcccgg ctggagcacc aactcgccgc cgagaaaaag actgactatt ccacgttccc
     5221 ggagatctaa atatggatct attgattgtt ttaacttacg tggcgctggc gtgggcggtc
     5281 tttaaaatct tccgcattcc ggtgaatcag tggacgctgg cgacggcggc gctgggaggc
     5341 gtgtttctgg tgagtggttt gattttgttg atgaactaca accaccctta cacttttacc
     5401 gcgcaaaagg cagtgatagc gatccccatc acgccacagg tgacgggaat tgttactgaa
     5461 gtcaccgaca aaaataatca gcttattcaa aagggcgagg tgctttttaa actcgacccg
     5521 gttcgttacc aggcgcgagt tgacagactt caggctgacc tgatgacggc gacgcataat
     5581 ataaagacgc tgcgcgcgca gctcactgaa gcgcaggcca acaccaccca ggtttcagcg
     5641 gagcgcgacc gtctgtttaa aaattatcaa cgttatctga aaggcagcca ggcggcggtg
     5701 aatccgttct cggaacgtga catcgacgat gcgcggcaaa atttcctcgc gcaggatgcg
     5761 ctggtgaaag gctcggtggc ggagcaggcg cagatccaga gccagctcga tagtatggtt
     5821 aacggcgagc aatcgcagat tgtgagctta agagcgcaac ttactgaagc aaaatataac
     5881 cttgagcaga ctgtcattcg cgcgccgagc aatggctacg ttactcaggt actgatccgc
     5941 ccaggtacat acgcagctgc cttgccgttg cgtccggtga tggttttcat ccccgagcaa
     6001 aaacggcaaa ttgtcgccca atttcggcaa aactcgctgt tacgtctgaa acccggcgat
     6061 gatgcggaag tggtgtttaa cgcgctacct gggcaggtgt tccacggcaa actgaccagt
     6121 attttacctg tcgtgccagg cggttcttat caggcgcagg gggtattgca atcattaacg
     6181 gtcgtgcccg gcacggacgg tgtgctggga accattgaac tggaccctaa cgatgatatc
     6241 gatgccttac ccgacggcat ctacgcccag gtggcggttt actccgacca tttcagccat
     6301 gtttcggtga tgcggaaagt gctgctaaga atgaccagct ggatgcatta tctttatttg
     6361 gatcattgag acttatatca cctgtgtttt gcgtgagcct gagccatact gatatttttc
     6421 ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa
     6481 actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta
     6541 taacgcggac aacggcgtgg cgcaatttaa cccgttaggg aaagtgccgg tgctggtgac
     6601 cgaagagggc gaatgttggt ttgattcgcc gatcatcgct gaatatattg aattaatgaa
     6661 tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga
     6721 ggcgctggcg gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc
     6781 ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct
     6841 ggatgtgctg gaaggatatc tggtcgatgg cacgctcaaa accgatacgg tcaatctggc
     6901 gactatcgcc attgcctgtg ccgtcggata tctcaatttc cgccgcgttg cgccaggctg
     6961 gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt
     7021 tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt
     7081 tacaatccgt ggttatgtta aacgcccttc tccgtgtgag agggccttga tcagccaggt
     7141 ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat
     7201 tgcgcgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat
     7261 tgctgcgtca gatgcttgac gaagcgcgag aagtgattcg tgacagccag acgctgcccg
     7321 catggtgtga aaactgggcg caagaagtcg atgcccgact gacaaaggag gcgcagagcg
     7381 cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca taccaatctt gggcgagctt
     7441 tacaggcgga agccgcagtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg
     7501 agtacgatct ggacgacgcc ggacgcggac atcgcgatcg ggcgctggcg cagctgctgt
     7561 gccgcattac gggagcggaa gatgcctgta tcgttaataa caatgcggcg gcggtgttat
     7621 tgatgttggc ggccactgct agcgggaaag aggtggtggt ttctcgcggc gaactggtgg
     7681 agattggcgg cgcgtttcgt attcctgatg tcatgcgtca ggcaggctgc accctacacg
     7741 aagtagggac caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata
     7801 ccgcactgtt gatgaaagta cataccagta actacagcat tcagggattc accaaagcga
     7861 tagatgaagc ggaactggtg gcgctcggca aagaactgga tattcccgtg gtgactgatt
     7921 taggcagtgg ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc
     7981 aggagttgat tgcggcgggc gtcagtctgg tgagtttctc cggcgacaag ttgttaggcg
     8041 ggccgcaggc agggattatc gttggtaaaa aagagatgat cgcccgcctg caaagccacc
     8101 cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacggtgc
     8161 gtctttattt acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc
     8221 gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggctcccctt gccgcacatt
     8281 acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc
     8341 tgccggttga tcgcctgccg agcgccgcat taacgtttac accccatgat ggacgcggta
     8401 gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg attggtcgta
     8461 tttatgacgg acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg
     8521 agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt
     8581 attgcaggcg attactggcg taaatgccga ccgtctgccg gaagaaaaaa agcgcggcat
     8641 gaccatcgat ctcggctatg cctactggcc gcagccggat ggccgtgtgc ctggttttat
     8701 cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gctggcgttg gtggtatcga
     8761 tcacgcgcta ttggtggtgg cgtgcgatga cggcgtgatg gcacagaccc gtgagcatct
     8821 ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca aagccgatcg
     8881 cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc tgcgggaata
     8941 cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc
     9001 cctgcgcgag catttgcttc agttaccaga acgtgaacac gccagccaac atagtttccg
     9061 cctcgcgatc gaccgtgcat ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc
     9121 gttaagcggg gaagtgaagg taggcgattc actatggctg actggtgtaa ataaaccgat
     9181 gcgcgtgcgt gcgctgcatg cgcaaaatca gccaacagaa actgcccatg ccggtcagcg
     9241 tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attcaccgtg gtgactggct
     9301 gcttgccgat gcgccgccgg agcctttcac acgggtgatt gtcgagcttc aaacccatac
     9361 accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg tcacgggacg
     9421 cgtttcgctg ctggaagata accttgccga gctggtgttc gacacgccgc tatggctggc
     9481 ggataacgat cgcctggtgc tgcgcgatat ctcggcccgc aacacgctgg cgggggcgcg
     9541 tgtggtgatg cttaacccgc cgcgtcgcgg taagcgtaag ccggaatatc tgcaatggct
     9601 ggcgtctctt gcacgggcgc agagcgatgc cgatgcgtta tctgttcatc tggaacgcgg
     9661 agcggttaac cttgcggatt tcgcctgggc acggcagctc aacggcgaag ggatgcgcga
     9721 actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc
     9781 cgcccgctgg cagcggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga
     9841 tgaacctggt cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc
     9901 gctggtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg
     9961 ttggctgcat ctgccggatc ataaagcggg cttcagcgaa gagcagcagg tcatctggca
    10021 aaaagcagag ccgctgtttg gtgacgaacc gtggtgggtg cgtgacctgg caaaagagac
    10081 gggaaccgac gagcaggcaa tgcgcctgac gctacgccag gcggcgcagc aggggataat
    10141 taccgcgatc gttaaagatc gttattaccg taacgatcgg attgtcgagt ttgccaatat
    10201 gatccgcgat ctcgatcagg aatgtggttc aacctgcgcg gcggatttcc gcgatcgttt
    10261 aggtgttggt cgaaaactgg caattcaaat tctggaatat tttgaccgca ttggttttac
    10321 gcgtcgtcgg ggaaatgatc atttattgcg cgacgcatta ttatttccgg aaaaataagg
    10381 aaatgattaa ttaagtttta aaataaatta atacaaaatt cttatgaatt taaaaaaagc
    10441 acattgttta atgaatacaa tgtgcttttt attagattaa tt
//
LOCUS       DAYPHN010000162        10352 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000162 DAYPHN010000000
VERSION     DAYPHN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.162"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..175)
                     /locus_tag="ACG0BW_003955"
     CDS             complement(<1..175)
                     /locus_tag="ACG0BW_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260856.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQ"
     gene            complement(567..1616)
                     /gene="yhhT"
                     /locus_tag="ACG0BW_003956"
     CDS             complement(567..1616)
                     /gene="yhhT"
                     /locus_tag="ACG0BW_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFK7260857.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1715..2965
                     /gene="yhhS"
                     /locus_tag="ACG0BW_003957"
     CDS             1715..2965
                     /gene="yhhS"
                     /locus_tag="ACG0BW_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7260858.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEVPEAISSS"
     gene            complement(2969..3526)
                     /gene="dcrB"
                     /locus_tag="ACG0BW_003958"
     CDS             complement(2969..3526)
                     /gene="dcrB"
                     /locus_tag="ACG0BW_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFK7260859.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3599..4264)
                     /gene="yhhQ"
                     /locus_tag="ACG0BW_003959"
     CDS             complement(3599..4264)
                     /gene="yhhQ"
                     /locus_tag="ACG0BW_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFK7260860.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            4485..4730
                     /gene="tusA"
                     /locus_tag="ACG0BW_003960"
     CDS             4485..4730
                     /gene="tusA"
                     /locus_tag="ACG0BW_003960"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFK7260861.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKSG"
     gene            4945..5253
                     /locus_tag="ACG0BW_003961"
     CDS             4945..5253
                     /locus_tag="ACG0BW_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260862.1"
                     /translation="MDYLIPVCLALLGLLAIFTPNWLIPVFIVVGGWLGVLAWGWLWL
                     MIFLPLLNGVGLAYALWLMFKRPSIASRLVAACCVVSVILMLALYYAFLTMPPFPPQH
                     "
     gene            complement(5286..7484)
                     /gene="zntA"
                     /locus_tag="ACG0BW_003962"
     CDS             complement(5286..7484)
                     /gene="zntA"
                     /locus_tag="ACG0BW_003962"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFK7260863.1"
                     /translation="MSTPDNHGKKAPHFAAFKPLTTAQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAVSWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPAVAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAATIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(7558..8184)
                     /gene="yhhN"
                     /locus_tag="ACG0BW_003963"
     CDS             complement(7558..8184)
                     /gene="yhhN"
                     /locus_tag="ACG0BW_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFK7260864.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            8325..8684
                     /gene="yhhM"
                     /locus_tag="ACG0BW_003964"
     CDS             8325..8684
                     /gene="yhhM"
                     /locus_tag="ACG0BW_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFK7260865.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTMGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(8687..8956)
                     /gene="yhhL"
                     /locus_tag="ACG0BW_003965"
     CDS             complement(8687..8956)
                     /gene="yhhL"
                     /locus_tag="ACG0BW_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFK7260866.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8946..9542)
                     /gene="rsmD"
                     /locus_tag="ACG0BW_003966"
     CDS             complement(8946..9542)
                     /gene="rsmD"
                     /locus_tag="ACG0BW_003966"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFK7260867.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDVD"
     gene            9692..>10352
                     /locus_tag="ACG0BW_003967"
     CDS             9692..>10352
                     /locus_tag="ACG0BW_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="cell division protein; signal recognition protein
                     receptor; functions in the targeting and insertion of
                     membrane proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFK7260868.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVVIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FR"
BASE COUNT         2503 a         2702 c         2735 g         2412 t
ORIGIN      
        1 cttgtccggc ttcggacata atccatttgc gttcaacgcc agctttcaga atcgcttttt
       61 tcacttgttc ctgggtatga ccagcgctga cggttgagtg aacctgatca attggcgcag
      121 tacgcgcgca acccgctaaa gcacccacaa cagcaatagc cgcaaaccat ttcatgtaat
      181 gtctcattag aaccccatta aagtattgtt tttacgagta aagcaagatt taactgtaat
      241 tgctggcatc actattcttt atttagcgaa aaaattactt gtgagataag tcagtaagtg
      301 cacatcagag taaaaatttc tgaaatgact aaattactgc cagcggggac caactataag
      361 tgagtgagaa aaatgaggca ataaaaataa cacaatgtta tcagtgcatt ctttgagaag
      421 gatttaatcg ctattcggcg tttatatata tttgacttaa ggaatggttt ttataaatta
      481 attgtctgaa taatgtttct tcatttcctc aaatcaaatg gccactgtta gataaacggt
      541 ggccattgcc tgctcaggag gtgctattaa cccggtaatc gacttttcgg tctgcccggt
      601 cccagtaaaa tcgccagttt gctaccgcct ttggtggttt ccatccagat tttacacacg
      661 ctggttaatg gtaccgaaag tagcatccct accgggccga gcagccatcc ccaaattaac
      721 aatgaaagaa ataccaccat ggtggacatc cccaggcgat ggcccatcat ccgtggttct
      781 aaaatattgc cgatgaccat atggaccact aaaaacaatg cgccgaccag aatacattcg
      841 taaacgccat taaacagcag cacctgaatc attggcggta cggcggaaat taccgcgccg
      901 atattgggca cgtagttgag caaaaacgcc agtactgccc acatcagcgc aaactgtacg
      961 cccattagcg ccagccccag ccagacgatt acgcctgtcc ataaacttag cagcgtcttt
     1021 aacgccagat aatgcgaaac accttttaag gcgcggtgta gtccggcgat gtgaatctgt
     1081 ggattattca acgcaaaacg cattttgtaa gggacgtggc gcacttcaaa cagcatgaaa
     1141 actacggtca tcaccagcaa aagcacgctc gccattgccc cggaaagccc ggtcattagc
     1201 gccgtggtga aggtaaccac tttttccgag tccatccgct gcagcattcg ctccggtgac
     1261 atatgcaaat taagaaaagg caacatctcc tgcaatttaa aaagtttgcg cgttagctcc
     1321 ttattaaact tcggcagcat agagataaat tcgttaaacg atgccgccag tacgccgacc
     1381 agcgcagtta gtgcgatcag catcaccacc accacaatcg taatggcaac ggggcgttgt
     1441 actccccgac gaataaacca ggtgacgagc gggttgagga cgatggcaaa aaacagcgcc
     1501 agtaacagct gcacaatgat atctgccgct gcgtgaatgc ccgcgaggat cactaccagc
     1561 gaggccagct tgagcagaat gtgcatgccc gttttatcgg gttgaggggt ttccattggg
     1621 gcttccttgt gactttttgt attaagtgta gcgggagtca cgccagcaat attctgattc
     1681 ttactgctga ttttcacgtc agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt
     1741 gatcctcatg cccgaacccg tagccgaacc cgcgctaaac ggattgcgcc tgaatttgcg
     1801 cattgtctct atagtcatgt ttaacttcgc cagctacctc accatcgggt taccgctcgc
     1861 tgtattaccg ggctatgtcc atgatgtgat gggctttagc gctttctggg caggattggt
     1921 tatcagcctg caatatttcg ccaccttgct gagccgtcct catgccggac gttacgccga
     1981 tttgctgggg cccaaaaaga ttgtcgtctt cggtttgtgc ggctgctttt tgagcggtct
     2041 ggggtatctg acggcaggat taaccgccag tctgcccgtc atcagcctgt tattactttg
     2101 cctggggcgc gtcatccttg ggattgggca aagttttgcc ggaacgggat cgaccctgtg
     2161 gggcgttggc gtggttggct cgctgcatat cggacgggtg atttcgtgga acggcattgt
     2221 cacttacggg gcgatggcga tgggtgcgcc gttaggtgtc gtgttttatc actggggcgg
     2281 cttgcaggcg ttagcgttaa tcattatggg cgtggcgctg gtggccattt tgttggcgat
     2341 cccgcgtccg acggtaaaag ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg
     2401 gcgcgtctgg ctgtacggta tggcgctggc actggcttcc gccggatttg gcgttattgc
     2461 cacctttatc acgctgtttt atgacgctaa aggttgggac ggtgcggctt tcgcgctgac
     2521 gctgtttagc tgtgcgtttg tcggtacgcg tttgttattc cctaacggca ttaaccgtat
     2581 cggcggctta aacgtagcga tgatttgctt tagcgttgag ataatcggcc tgctactggt
     2641 tggcgtggcg actatgccgt ggatggcgaa aatcggcgtc ttgctggcgg gggccgggtt
     2701 ttcactggtg ttcccggcat tgggcgtagt ggcggtaaaa gcggttccgc agcaaaatca
     2761 gggggcggcg ctggcaactt acaccgtatt tatggattta tcgcttggcg tgaccggacc
     2821 actggctggg ctggtgatga gctgggcggg cgtcccggtg atttatctgg cggcggcggg
     2881 actggtcgca atcgcgttat tactgacgtg gcgattaaaa aaacggcctc cggtggaagt
     2941 tcctgaggcc atctcatcat cttaaaactt actgaataac cagcgtatta atgatgtttt
     3001 ctgcggtggt ctgtgctttt tgctgatcgt cagctggcag cgtaatttgc atggtcagca
     3061 gttgattacc cacgttaccc agaataacgg aagagtacgc cgtctggcct ttcgcggaga
     3121 taatgctgtc taactgctgc attttgtgac ctttcagctc aatggcttta ttggttacca
     3181 cttgcagctg cggatcgcgg ctacgttgct gatcttccag gcgcttcgcc agcaccgcca
     3241 gatcttcttt cggatcatcg cccatgatga cgatgactgc tttttgcccg gtggcgtcgg
     3301 accagacatg catgttatta gcctgcgttc ccagcttacc gctctggtcg gtcatatccg
     3361 ctggcagcga gaaacttaac ttgccatcaa gcaggttgac gggattcccg gtagcgttac
     3421 tttccgcgac cgaaccctgc gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc
     3481 ccataaccag caggccaatt ccgacatatt taaccagatt gcgcattgac ttcttccttt
     3541 cgataaacgg ccataacggc tcattcatcc atcttatcac aactctgata acgaaccttt
     3601 aactcgcctg caaagcgttg atttcggatt tatctgccag tcttttcaac agcatattga
     3661 gtaatacgcc atacattggt aggaagaaaa cgatactgat taacactttg aaacagtaat
     3721 cgaccagcgc gatttccatc cagtgttcag ccataaaggc atccgggcta cgccagaagg
     3781 caatgaagaa aaaggccagc gtgtcgctga cgttaccgaa cagtgtggaa gccgtcggtg
     3841 ccagccacca gcggcgactc tgacgcaggc ggttaaaaac gtgcacgtcg aggatttgcc
     3901 ccagcgcgta ggccatgaaa ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg
     3961 cgagcgcgcc gaatccctgc caggaaccca tatagaacag cgacgagatg acgtaggaga
     4021 tcagtaacgc cgggatcatc accgcaaaga tgatacgacg ggccagcggt gcgccaaaaa
     4081 tacgcacggt caggtcggta gcaaggaaaa taaacggaaa gctaaacgcg ccccaggtgg
     4141 tatggaaacc aaaaatggag acgggaagct gaaccagata gttactggag gtgatcacca
     4201 aaagatgaaa taacgataac cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt
     4261 tcatattgta cctttttgat taaccattgg ggtgagggaa cccaatacgt acgacacgtc
     4321 tgttacctta acataataac gtgctgaccc ttttttcgag ccgccgcatg atactgcttt
     4381 acgttgacaa tgcaatggtt aattttcacg caatcgttaa cctggtttgc ttacggactc
     4441 acagggcgta aactagcgcc gtttttttat gtgatgagaa gaaaatgacc gatctctttt
     4501 ccagccctga ccacacactc gacgcgcttg gcctgcgctg cccggaacca gtaatgatgg
     4561 tgcgcaaaac tgtgcgcaat atgcagccag gtgaaacgtt gctgattatt gctgacgatc
     4621 cggcgacgac ccgcgatatt cctgggtttt gtacctttat ggaacatgag ctggttgcta
     4681 aagagacgga tgaactgcct tatcgttatt tgattcgcaa atcggggtaa ggtggatcag
     4741 gctagcggac cgatcgcgct caattttgcg gcttgcaacc ggtttgtagg acggataaaa
     4801 cgttcaagct gcatctggcg atacggtgcg caatgcctga tgcgacgctg tcgcgtttta
     4861 tcatggctac gagttcgatg ccctgtgtag gctggataag gcgtttacgc cgcatccggc
     4921 aagcaagtgt aatggaatgc acaaatggat tatttaattc cagtttgtct ggcattgctg
     4981 ggattactcg cgatcttcac ccccaattgg ctgatccctg tttttatcgt cgtgggggga
     5041 tggctggggg tacttgcctg gggttggctc tggctaatga tttttttacc tttacttaac
     5101 ggtgtcgggc ttgcttatgc cctgtggttg atgttcaaaa ggccatctat agcatcccgc
     5161 ctggttgctg catgctgtgt tgtctctgtc attctgatgt tagcgcttta ttatgctttc
     5221 ctgactatgc cgccgttccc gcctcagcac taagcctgat agccagaggc tatcaggcaa
     5281 cacttttact tcctgcgcaa cagccttaac gcattcgctg tcaccagcac cgtcgcaccc
     5341 gtatctgcca gcaccgccag ccatagtccg gtcatcccta acagcgtggt gacgaggaag
     5401 atccctttca gccccagcgc aatggtgatg ttctggcgga tattggcgtg agtggcgcgt
     5461 gccagttcaa tcatttgcac caggccgcgc aggtggttat gggttaatgc tgcatcggcg
     5521 gtttccagcg ccacgtctgt gccactgccc attgcaatcc caatggtggc ggctttcatc
     5581 gctggcgcgt cgttaatacc gtcaccgacc atcgccagcg gcgcatgttg attcagctcg
     5641 gtcaccgctt tgactttatc ttccggcaac aggcccgctt taaactccag ccccagttcc
     5701 ccggcaattg ccgccgctgc gcgtggatta tcgccggtga ggatcacccc tttgacgccc
     5761 agcgcgttca gttcactgat ggcagttgca gcatcggcgc gcagggtatc ctgcaatgca
     5821 attacgccaa gcacgtcatc gttacgcact accagcacta ccgtttgccc ggcgctttcc
     5881 agttcattaa tcaaaccagc aaatgcaaca gccggatgtt ttccggcagc gcatatcaac
     5941 acgcgctcac cgttaacctg cgcttcaatg ccagacccga ccagcgcccg ctgtgattcg
     6001 gcggcgggaa tggcgagttc agcaacctgt gcttcgcgta cgatggcttg cgccagtgga
     6061 tgcgtcgcgc cttgctcgac cgccgctgcc agtgtcagca gttcagattc actaataccc
     6121 gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg tcagcgtacc ggttttatca
     6181 aacgccacct gggtaacacg acctagttgt tccagcgccg caccgccttt aatcaacgcc
     6241 ccacgacgcg ctgctgctgc cagcccggag gttatcgccg caggcgttga gataactaac
     6301 gcacacgggc aaccaatcag cagcagcgtc agccctttat aaatccactc ttgccagctg
     6361 gccgcaaaca gcagcggcgg caccagcgtt accagcaggg cgacggccat aatcgcgggc
     6421 gtgtagatac ggctgaaacg gtcgataaac cgctcaatgg gtgcgcgacg ctcttcggct
     6481 tcttcgatca gtttcagaat ccggtcaatg gcgctggcac ccggttctga cagcacttcc
     6541 agcgttacca gacggtctac gctggtggct cctgcaggaa ctttatcacc cgtcgcgcgc
     6601 tccaccggaa tggattcgcc ggtcagggcg ctttcatcaa aactggcaaa cggtgagagc
     6661 agtttaccgt cggcaggcaa acgcccgcct gcggcaactt caatcacatc gccagggcgc
     6721 aggctgttaa tcgccacctc ttcacgctca ccgttacgca ggcgcgtggc ggtttctggt
     6781 ttcagtgcca ttaacgcgct aacgccctga cgcgcgcggc tggcggccca gccttccagt
     6841 cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt cagccgttgc gccaataaac
     6901 agcgcaccaa tagcggctac gctcattaag gtttcaatag cgaagtagct gccggatttg
     6961 atcagccgta atgcctgacg agcaatcggg tacagtccaa ccagcgtggt cgcgataaac
     7021 gccagttgcc cgaacggatg attaaactgc tccagtcccc aacttaccgc catcatcacg
     7081 ataagcgtaa tcagcggcaa attctctttc aggcgtgatg cttgcggttc gtcggcggcc
     7141 tgttcatcgc gcagggaata gcccgctttt tgcaccgcag attcaacctg ggcacggatg
     7201 tcattgtcgg catcgaccac cagtttttcg gtggcgaaca acacctgcac ctgattcacg
     7261 cctgcaagct ggcgcacggc attttctacc ttgcgcgcac aggcggcgca gtccatgccg
     7321 ctgactttcc agctatagcg ggtgccggag acgttttcag agagcgttgg cgtgctggaa
     7381 catgcgccgt cgcagcaaca gtcgttggcg ttctgtgccg tggttagcgg tttgaacgca
     7441 gcaaagtgag gggctttctt gccgtgattg tcaggagtcg acatggcatc ctccggttaa
     7501 gttttttctc attaaccgaa ggatacactc tggagtcgac tccagagtca agttttatca
     7561 gagatacagc gagcgaacga tgaggaaatg accggcaaag tagcaggccg cagcaatagc
     7621 gttgtccgca cggaagcgtc ggcgatagtg actacccagc cagacaaagt tactgataaa
     7681 cagcaacgaa gcgccgacaa acgcagagag cgccggagcg gtcggacgga agaaccacag
     7741 ttcacctgcc agccacacca tcaccagcgt catgccgata aacgtgcaga taggccaacg
     7801 atactcttcc agacgcgtcc agataatcgc cagtaacagc gcgcccagaa ccagcaacac
     7861 cagtggcaga ggccagaaga aagagagcgt catctgactg gcgaaataga tggtgtacag
     7921 caggtgcgag aggaaaaacg cgccgatggc gtacatcaga cgttgacgtg gcaacagggt
     7981 tagcgcatcg cccagcagtg aggcgcacag ccccgccagc accagatagc taatggcgtc
     8041 gaacattggc gcttgccagg ccagtaacag caggagaaga agggttaacg gtttaaacac
     8101 ccagcgttgc caggttggcc cacgatacga tgcatccaca gatagccatg cggaaagaca
     8161 gacagcgata aacgaccaaa gcatcttagt tccttgaatt cgttattttt tcgtaagcgg
     8221 aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg acggatgaca acaccataac
     8281 tggcagggta tgcttaattc cgcattttcc gatgagggat aaagatgagc aaaccacctc
     8341 ttttctttat tgttatcatt ggcttaatca tcgtcgccgc atcgtttcgt tttatgcaac
     8401 aacgacggga aaaagctgat aatgatatgg caccgctcca gcaaaagctg gtggtggtga
     8461 gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg ccagcaggaa gtgactccgg
     8521 caggcaccag tatgcgctat gaggcaagct tcaaaccgca aagcggagga atggagcaga
     8581 cgtttcgcct cgacgcccag cagtaccacg ccctgacaat gggcgataaa ggtacgctga
     8641 gctataaagg aacgcgcttt gtcagctttg ttggcgaaca ataaggttat tttttaactt
     8701 taaatttctt ctgccagacc agcagttcaa acacgccgaa aaggaaaatc cgcacctttt
     8761 cggcggtggt catctgcggg ccatctttcg gtaaagtgga tttcaatagc gccagttgca
     8821 taccatgcat cagcacggta aaaatcagcg ccacgttaac gaaaatattc agcggacgcg
     8881 ggaagggatg caccaggttg aggattaaaa atccccaaac gcagagcatt aacaaacgtc
     8941 caatattaat caacatcact ttctccttgt gcttcgcgtt gatacagccg ataagccacc
     9001 tgacccgcca ctttttcccg atgtaatgac cagtttgccg gaacagtggg cagaccgttt
     9061 tcgacttcgc tttcgacata aatcagggct tcgtcagcca gccagccgtt atcttccagt
     9121 aaatttatcg tctcttctaa caagccacgg cggaacggtg gatcgacaaa cacgatatta
     9181 tgcggtgtgc ctttttgcgc caggaatgac atcgcgttgc tgttcaccac gcgtgcattg
     9241 cctgctttta gtgtcgccag attcttaatt aactgctgag aaaccgcgcg atccatttca
     9301 atcaatgtgg cccccgcagc gtagcgcgat aacgcttcca gccccagcgc gccgctcccg
     9361 gcgaagcaat ccagacattg ggcgtcaaca atgaccggag ccagccagtt aaacaacgtt
     9421 tcgcgtacgc ggtctgtggt ggggcgcaga cctgggctat caggaaccgg gagtttacgg
     9481 cctcgccact gcccgccaat aatgcggatc tggccgctgc cggaatgatt cggttttttc
     9541 atgataaatt gctcaatccg ccagataaca catacttgca ggcggtgatg tgaattaagt
     9601 taagtgatag actatttcat cattttttta gctgctatgt acatagcgtt aacgctgtgc
     9661 catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa gaaaaaaaac gtggcttttt
     9721 ttcctggctg ggctttggtc aaaaagagca gaccccggaa aaagagacag aagttcagaa
     9781 tgaacaaccg gttgtagaag aaatcgttcc ggcgcaagag cctgcgaagg cttctgaaca
     9841 agccgttgaa gagcagccgc aggcgcatac tgaagccgag gcggaaactt ttgctgccga
     9901 cgttgtggaa gtcactgaac aggttgctga aagtgaaaaa gcgcagcctg aagcggaagt
     9961 cgttgcacag ccggaaccgg tcgtagaaga aacgccggag ccagtggtta tcgagcgtga
    10021 agagctgccg ttgccggaag acgtcaacgc cgaagaggtt tcgccagaag agtggcaggc
    10081 cgaagcggaa accgtagaga ttgtcgaagc ggcggaagaa gaagcggcta aagaagaaat
    10141 caccgacgaa gagctggaag cacaggcgct ggctgccgaa gcggcagaag aggcggtgat
    10201 ggtggttcct ccggcagaag aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa
    10261 accgaccaaa gaaggtttct tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa
    10321 cctcggttcc ggatttatca gcctgttccg cg
//
LOCUS       DAYPHN010000163        10283 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000163 DAYPHN010000000
VERSION     DAYPHN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.163"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            195..467
                     /gene="ynjH"
                     /locus_tag="ACG0BW_003968"
     CDS             195..467
                     /gene="ynjH"
                     /locus_tag="ACG0BW_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFK7260869.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(433..840)
                     /gene="nudG"
                     /locus_tag="ACG0BW_003969"
     CDS             complement(433..840)
                     /gene="nudG"
                     /locus_tag="ACG0BW_003969"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFK7260870.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEAAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            927..1547
                     /gene="ynjF"
                     /locus_tag="ACG0BW_003970"
     CDS             927..1547
                     /gene="ynjF"
                     /locus_tag="ACG0BW_003970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFK7260871.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1556..2863)
                     /gene="ynjE"
                     /locus_tag="ACG0BW_003971"
     CDS             complement(1556..2863)
                     /gene="ynjE"
                     /locus_tag="ACG0BW_003971"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFK7260872.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKLHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(2933..3583)
                     /gene="ynjD"
                     /locus_tag="ACG0BW_003972"
     CDS             complement(2933..3583)
                     /gene="ynjD"
                     /locus_tag="ACG0BW_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFK7260873.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKCDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAERQIGILFQDALLFDQFSVGQNLLLALP
                     AALKGVARRDAVNDALERAGLGGMFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRKWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKL"
     gene            complement(3583..5118)
                     /gene="ynjC"
                     /locus_tag="ACG0BW_003973"
     CDS             complement(3583..5118)
                     /gene="ynjC"
                     /locus_tag="ACG0BW_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFK7260874.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALMATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGEADQQTKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASLLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSQSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(5091..6257)
                     /gene="ynjB"
                     /locus_tag="ACG0BW_003974"
     CDS             complement(5091..6257)
                     /gene="ynjB"
                     /locus_tag="ACG0BW_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFK7260875.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWVETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(6267..6815)
                     /gene="ynjA"
                     /locus_tag="ACG0BW_003975"
     CDS             complement(6267..6815)
                     /gene="ynjA"
                     /locus_tag="ACG0BW_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFK7260876.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(6815..7522)
                     /gene="ydjZ"
                     /locus_tag="ACG0BW_003976"
     CDS             complement(6815..7522)
                     /gene="ydjZ"
                     /locus_tag="ACG0BW_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFK7260877.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(7537..8214)
                     /gene="ydjY"
                     /locus_tag="ACG0BW_003977"
     CDS             complement(7537..8214)
                     /gene="ydjY"
                     /locus_tag="ACG0BW_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFK7260878.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQQENAGAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSLDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(8219..8929)
                     /gene="ydjX"
                     /locus_tag="ACG0BW_003978"
     CDS             complement(8219..8929)
                     /gene="ydjX"
                     /locus_tag="ACG0BW_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFK7260879.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(9096..9902)
                     /gene="xthA"
                     /locus_tag="ACG0BW_003979"
     CDS             complement(9096..9902)
                     /gene="xthA"
                     /locus_tag="ACG0BW_003979"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFK7260880.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSTLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2532 a         2721 c         2625 g         2405 t
ORIGIN      
        1 tgtttatgtc atgtgctttt gcagttttcg actataccat caagaatgtg acggtaagca
       61 agaaaaaaat gacccaggaa agcaaattaa taacgagagt aatctcataa ccaaatgcga
      121 taactcagtc gctaaagtta tgccgcccgg ctaccgttat tagtagcaga gacaataacg
      181 ttaaggagtt ccctgtgagt cgagcattgt tcgctgtagt attagcattt ccattaattg
      241 cactggctaa cccgcattat cgtccggacg tggaggtgaa cgtaccgcca gaagtgttca
      301 gttcaggcgg gcagtcagcg cagccctgta ctcagtgctg cgtttatcag gatcaaaatt
      361 attctgaagg ggcggtaatt aaagccgaag gaatactgct gcaatgccag cgggatgaca
      421 aaacccttag cactaatccg ctggtctggc ggcgcgtaaa gccataaatg cctctaatag
      481 tggaatgtca gcaggggcca gcggatattg cagcgcctct tcaggtgagc accagaccag
      541 cgcctgatgt tcatgtgcct gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag
      601 atggataatc cgccccgaaa cttctcgctg atggctggca acatattcac ccacagctgc
      661 ttcgatgccc agttcttcgc gtaactcacg caccagcgcc tgccgctggc tttcatccgg
      721 ctcgacttta ccaccggcaa actcccataa tcccgcctga tcgctatggg cggggcgttg
      781 cgcgagtaaa attttgccat cacgttcgat gatggcggca acaacttcaa tcattttcat
      841 aaattacaca cgggcagata agcgaaaaat gccataatat ccgcactgtt gctcaatcag
      901 aaaggtttta tatttaagga gtgcctgtgc tagaccgcca tcttcatccc cggattaaac
      961 cgttgttgca tcagtgcgtg cgggttcttg ataaaccggg cattacgcca gatggtttaa
     1021 cattagtggg atttgccatc ggtgtgctgg cactgccttt tctggcgctg ggctggtatc
     1081 tggcggcgct tgtcgtcatt ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg
     1141 cgcggcgcag agggcttacc gacgcgggcg gctttcttga tatttctctc gattttcttt
     1201 tttacgcgct ggtacctttt gggtttatcc tcgccgcacc gcagcaaaat gcgctggcag
     1261 gcggctggct gttgtttgcg tttatcggta cgggcagcag ttttctcgcc tttgctgcac
     1321 tggcagcaaa acatcagatt gataaccccg gttatgcaca taagtcgttt tactatctgg
     1381 ggggattaac cgaaggcact gaaacgatct tactgtttgt gctgggatgt ttattccctg
     1441 catggtttgc gtggtttgcg tggatcttcg gtgcgttgtg ctggatgacg acctttacgc
     1501 gggtgtggag tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt
     1561 tgctgctgtc cgggccgcgt tcaccggttg ctaccggatt ttttggatcg ctgctccatt
     1621 cgtaccagcc gccgtcgtaa acggagacgt tattccagcc cattgctcgt gcgtacataa
     1681 aggtttcgga cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga
     1741 tattccatgc tttccacata gcggtaatat catcggcgct acgcatggtg ccatccgggt
     1801 tatggaaatc ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta
     1861 tttcaccttt tggtttaata tagctgtaac cgctggtcgt accgataaat tctggccacg
     1921 aacgaatgct caccagcgat gcatcctggc gatgcagcag tccacgcgcc tgttccatat
     1981 caagcatcag ttgcggttgt gccgggatct tcacgccgaa atccggttcc gctttcactt
     2041 tcggtggcgt tccgcgctca acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat
     2101 ccagcaggcg cacatctttc acgccggcat aaagcattat ctgcgccaca cgcgctgcag
     2161 cgtatacgtc acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca
     2221 gcatcgcttt tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt
     2281 tggtatcgat gtagtcagcg ccgggaatat gactgataag gtaaagctta ggagcgcccc
     2341 aggccgcttc aatgactttc cagtcaccgg caggtttcgc cgtaacctct ttaccttgtt
     2401 gcaggtcatg cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgcagac
     2461 gggaaggttc acttagcgcg tcactcagga tggagatatg cgtaaaacct gctttttgca
     2521 gacgcgtttt gacggcatcg acatctttgt cattaccgta cagcgcaacc ggagcatcgg
     2581 ctttcaggtt atgtagcttg atccacgcgt taagctgttc ggtgctcatt ttgtcgagcc
     2641 agctagcaga gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg
     2701 gccagccgtt ataaaacgcg ctggggcgag tatctatcgc tttgccattt tgttgttgaa
     2761 gctggtcaag tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc
     2821 ctaaagccat tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacattg
     2881 tagttttgag tgcgccattc tgaactgacg cattgagaaa aactttgcgt tatcacagtt
     2941 tgttgtaatt ttctgaccac tgcgccatat ccagaacaga actatcagca ggaacatcct
     3001 ggagatcgtg cgttacctga acgacgggga tcgccagggc gcgaacttcg ctaaataccc
     3061 acttgcgaaa attatcgcgc agagctacat caagacggct gaatggctca tccagtagca
     3121 acgcttttgg ctgagcgaga agagcgcgaa gcagagcaac gcgcgctcgc tgaccgccag
     3181 acaaagtggc aggatcctga tggaacatac cgccaagccc cgcacgctcc agcgcatcat
     3241 ttaccgcatc gcgccttgca acacctttga gtgccgctgg cagagccagc agtaaatttt
     3301 gcccgacgct gaactggtca aataacagtg catcctggaa aagaatgcca atctgacgct
     3361 ctgcggtggg tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa
     3421 actgtccggc cagcgcacca atcatccatg aaaacagagt ggattttcca cagccagacg
     3481 gccccattaa cgtgacaatg tcacatttat ccaccgtaaa gttaacgttt gtcagcaagc
     3541 agctttctgg taaacgtagc gaaacatttt tcacgcagag cattagcgga gtccttgtct
     3601 gacataacct acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaatag
     3661 cagttgccat aaagcctggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac
     3721 cgcttcagtg gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg
     3781 tgcaatactg actgaaaatc ccaccgcgaa ggcaatcagc gtagggcgta gcataagcgg
     3841 acatttcacg taaaagaaga ttttggactg cgaccagccc agtgtttgcg caatcaaaat
     3901 taaccgtgaa tcaatgcgct gccaggccgg ttgcaggata aacagcatcc acggcattac
     3961 ccacagcaga tgcccccaga ccaccgccgt ccagcttcca tccaggttta gccatagcgc
     4021 cagcgtgtac tggcccgcca ccagtggcag agcgggtaat aaaatgggca accatagcca
     4081 caactggcga cgctgtggcc cccattccag ccacagcagt aacaggagca aagcgatgaa
     4141 tgtcgccacc agccccattg tcaggctgtt gatgagcgct tcactattga tcgtcgactg
     4201 atccgcgagg atcgccagca gaaccacaca cagcacaccg gttaagggta aaagactcgc
     4261 cagcgtaatg ccaggcaata aaggcgtggc aggcttacga acgccatcta cgcggggaat
     4321 agtacgccgc cagctacgcc atagcagata gcccagcaaa acgtaggcgg cgagtaacag
     4381 catcagcagc aggctggcga gtgcgccttt cgtttgttga tcggcttcgc cctgagttaa
     4441 ccactgccag ctaattactg ccagcgtcgg tggattacct ggcccaagaa taatcgccac
     4501 atccacgacc gacagcgacc aggcaacaat cgccagcatc gccattgcca gcgcaggcgc
     4561 gacggagggc aacagtagcc agttcaggca ttgccagcgg ctgtagccca gcgaatccag
     4621 cacaatgagt tgctgcaaca gccgtttttc gctcaatact gccgctaaga tccacaatag
     4681 aaatgcgctt tctttcaccg ccagcgtgaa gcccagccca atacccaatt tgtccattgg
     4741 cggagtgaaa tatgggaaat agtcataaag cagccctccg tcagcaaaga gcagaagggc
     4801 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4861 ctgccatttc ggcccaggcc acagcgccac aatcaccagc agggcaatca acaacgcccc
     4921 gaccgccgcg atggttgtcg acaccagcgt tgccattaat gcctgcggta actgtggatc
     4981 ggcaaataac gcctgccagt gtgtcaacga caaagcaggc gatatcagcg tgagagctgc
     5041 cgggatcagc ggtgcataaa tcaccgccat catcgcccac agcaaaaaga ttaatgcgta
     5101 ccgtaacggt gtagccattc ttgttccagc gcatttaccc aacctgcgtg cggttcagcc
     5161 agtaccggcg gcagatcctg cggcattctc gattgcaatg tttcgcgctg cccgtcaggc
     5221 agtttttgcg gatcgagaac agaaggatcg ccccagacag cgggatctgc tttacgcagt
     5281 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5341 ttggcaggaa tggtgacaaa atgcacgttg ccaatcatcc cctcgttaaa accaaaactg
     5401 taactgcttg ccggcaaatc gccactggcg attttttgct gcgcatgcgc ggggttaaag
     5461 gtcagcgaca ggcgcaatgt gccagctttc agcagagcat ccatccgcgc gggcgaaggt
     5521 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgccacaag
     5581 ggcgcagtga cgcgggcgaa agtcgcatcg tccggcgctt cttttaatgc tgtgggatct
     5641 ggcgttagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc tggcgggcgc
     5701 ggataggtaa ccgtgccagg attggcttta gcaaactcca gcaacgcttg cggcgtttgt
     5761 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc accccagggc
     5821 gattcagccc cttctgtagg cactgaaaaa tcttcccgca ccggcagctg tgtgtcgaca
     5881 tagcgccagt tgggcagagt ctccacccag cccgtttgta ataaattggc ctcttttaag
     5941 gtgcggaagt tttcgccgtt cacccagagc agatccaccg aaccgcctgt tttgcgtccg
     6001 gcagcagctt cggtctgaat gcgcttcacc gcgtctgcgg catccgcgag acgaacaatc
     6061 ttcaggttta tagcgtaatg ggttttcatc tcgccgctaa cccagtcgag atagcggtta
     6121 attgccgtat cgccgcccca ggcgttaaac cagacggttt gccctttggc gtcgtttttg
     6181 atttcctgcc agtcactggc atgtgttgcc aaagaaaaca gcgataacaa tcccagcaac
     6241 cacacacaat ggcgcatcat ctctccttaa gaacgttttt caggaatacg gcacagcccc
     6301 tgagcgggaa tgtccatggc agaattaaaa cgagccgaca ggttttgcag gccaatcaat
     6361 gccgtcagtt cggtgagcgc ctgagcgtca aaatgcgtaa ccagtcgggc gcgcagggca
     6421 tcatcgaccg ttggcggcgt tacgcttgcg gcttcggcgt actccagcgc cagccgttct
     6481 tcatcgctaa agagcgggct ttgccgccag tcagccactg ccagcagttt atcggtgctg
     6541 ccagtgcgct cggcgacttt catactggtg atatccacgc aaaactcaca caggcacatt
     6601 tgcgcaatgc gcgcgctgac aagcgatcgt actaccggat ccagcggtga gcgtttgcgc
     6661 tccagccagc cgacaaacat cgacaccaga taaaagataa acgggatccg cccccaccag
     6721 cgaatcgggc ttagcacctc gccgtaatgg cgacgatgca gccacgcctg cggacgtaaa
     6781 atgaaaggaa ttttgctaag cggcggtaaa cccatcaggc attcctcttc tggcgctcaa
     6841 gccatatttt cttcgccata aaaatcacca cggtcagggc aaacagaata aacagtccgg
     6901 tgacaaacca gaaagtaccg cctgttaaca tgctgcccgc ccaggaataa acaatagtcg
     6961 ctggtaactg accaagcccg gtggcgataa aaaacgagcg aaaacgtatt gaagtcaaac
     7021 cggcagcata gcttacggga tcaaaaggca caaaaggcaa taatcggcat accagaatgg
     7081 tgtgtttacc atagcgggta aaaaagccgt ccatactatt gagtaccgtt ttacccgtta
     7141 acttttccac cacttcgcgg cccatcactc tggcgataaa aaagcacagc gccgcgccgg
     7201 ccatcgaact ggtccacgac agcaagccgc cccagaacgc gccaaacagc gacgcatttg
     7261 caaaggtgat cagaaacgca ggtagcggtg cagcaatggc ctgcaaaatc atcaacaaga
     7321 acgagacaac tgccgccagt gcgccgtaag actgaataaa gcgttctata ccctgttggt
     7381 ccacggcggc aaacgccgca acgctgcgat tgataaactc atggacgccg ggaagcagcg
     7441 cccatgccag cagcatagcg aacaacagga tgatgagggt tatacggtaa taccagattt
     7501 ttcgcgattg catcttcatc atcctttatt ctggctttat tcggtgattt taaaggtaac
     7561 cgttgccagc gtgttatccg ccgggagaac cgaggcattg cctttgaatt taacttcacc
     7621 tcgtttttca accgcaccat aagtgtatgt tgcattgctg acgatgccga ccgggcagct
     7681 atccagacac accaggcagc ccgttttctt ctcttctgct gccgttaaat taccgccaaa
     7741 gcgcatgtcc agtttcttgc cattactgtc aacaatcact tcatctaagg aatacgcttt
     7801 tgccgcccct tgccagttga ccgaaatatc cagtttgctg cctgtgacat gagtcgtttc
     7861 tttattatcc atcgtcatgt tttcgcccgg tgtaccacct gcctctttca gggcttcata
     7921 aaatgctttc ggggtcgcat aacccataaa cagcgattta tgtccgttgg agccatcttt
     7981 aaacacaata ccgtgacgag tgtcgtcggt gaggaaacga ccattaattt gcaccagcat
     8041 ggttaccgta tggttattgg catcgacacg cagcggttgg ctattcgaaa gtccgtcgta
     8101 ttccactttt gcacccgcat tctcttgttg atcgcagccg ctaagcccgg caacagccag
     8161 taagcagagt gcagccagtc cttttttcca tgacactgaa taatgttgca acatcgttct
     8221 atccttcatt tttggggtga gtaagtgggc tgcggcgcga agcagacaga tccacatgtt
     8281 tgtgtcgggc gtagagtttt gcgagctgga cgagaataaa cagcgccagg cccgccagac
     8341 agagttgtaa aataaagcgc agcgtaatgc cttcattggc gagatcgctt gccatcacgg
     8401 tataaataac aataccgggc agggtcgtga gtgccgaaat aagggtataa ggccagaagg
     8461 cgattgtggt taatccgtaa gcgtaatttt gaatattgta aggaaacaac gggattaagc
     8521 gggtcagaat aagaaaatca ataccattac gcgcaatgcc tttttcaatg gcctggaagg
     8581 tatggctatg gccaacgtat ttcagcagta aatcacgtcc cagccagcgc gccagcagga
     8641 atgagcacga ggaggccagc gtggcggcaa ttaatgagag tagtgtccct aagagcgggc
     8701 caaaaactat tcctccagcg atcaccagta tgcttcctgg taacagaaag agggtggcaa
     8761 tgatgaataa cagaatatag aggctataac cgaacaatcc gctctggcga atgagtgtct
     8821 gtaagtgggg caaatcggtg agcagatcga ataaaccgaa agcgtggata gcgtaaatga
     8881 gcagcgcaaa aataagacag gcaaaaagaa atttacgctc agcgttcatc gtggagtttc
     8941 ctatatcaaa cttgataata gagaagtggt cagacaacta aaaacgcagt gacggtataa
     9001 caaagggcgg caggcaacaa atcattactg acagtttgtt gatttttctc aaggttaatt
     9061 ctcctgaccc agtttgagcc aggagaacag ctaaattagc ggcggaaggt cgcccagacg
     9121 ggggcgtgat cggacggttt ttccatgctg cgaatttcat agtcgatgcc ggtttctacg
     9181 caacattctg ccagcggttg gctggcgaga agcaggtcga tgcgcagacc acggttatcg
     9241 tcaaaacctt ttgagcggta atcaaaccat gagaaacgat ctgctgtttg cggattcgca
     9301 tggcggaagg tatcgaccaa cccccagctc atcagtctct ccatccattc gcgttcctcc
     9361 ggaaggaaag aacattttcc ggtacgcagc cagcgcttac ggttctcttc gccaatgcca
     9421 atatccaggt cgccaggact gatattcata tcgcccataa tcagcaccgg attttcacgc
     9481 ttgagttcgg tttccaggta gttttgcaga ttctgataaa actgcgcctt tgccgggaat
     9541 tttatcggat ggtcgcggct ttcaccctgc gggaagtaac cgttgatcac ggtaacattg
     9601 cccagcgtcg aggggatttc agccataata attcgccgtt gcgcctcttc gtcatcaccc
     9661 ggaaaaccgc gacgcacggc aatcggcgtc tctttggtca gcagcgccac gccataatgg
     9721 cctttctgcc cgtgataaaa cacgttgtag ccgagcttcg ccacctcttc gagtggaaac
     9781 atatcgtcat gaacttttgt ctcctgcagg ccaatcacat ccggttggtg cttttcgacg
     9841 atggcttcaa gctggtgagg tctggcgcgc aggccgttga tattaaaaga gacaaatttc
     9901 atagtcgctg ccatttattc agataaagag tgcgtggatg gtagcagaat ttcgctttgc
     9961 gtaccgcctg ttgttaagtg atttgatcga tggttgttaa tgatgtcaac gatggcgcaa
    10021 aaaatgcccg cttttggtgc gcgttgcgtc agaatggcgc agtaatttcc agtaaatttc
    10081 gtcaatgatc acaattccag aattatattt tacttctgca taacattgcg ttttttattc
    10141 tttttattca cccacatcgc aaacgtattc actttatatg cactttaaat gcatatggtt
    10201 tggttataac tccttgattt ccagttagcc accgcggttt atgcactttt atcactggct
    10261 ggcacgaacc ctgcaatcta cat
//
LOCUS       DAYPHN010000164        10197 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000164 DAYPHN010000000
VERSION     DAYPHN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.164"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(45..662)
                     /locus_tag="ACG0BW_003980"
     CDS             complement(45..662)
                     /locus_tag="ACG0BW_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFK7260881.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISAIAEAVCQLPKLQEVLKRNVII"
     gene            929..2428
                     /gene="ansP"
                     /locus_tag="ACG0BW_003981"
     CDS             929..2428
                     /gene="ansP"
                     /locus_tag="ACG0BW_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFK7260882.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEQQEIVFKPETAS"
     gene            complement(2541..3602)
                     /locus_tag="ACG0BW_003982"
     CDS             complement(2541..3602)
                     /locus_tag="ACG0BW_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFK7260883.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3844..5946
                     /gene="pqqU"
                     /locus_tag="ACG0BW_003983"
     CDS             3844..5946
                     /gene="pqqU"
                     /locus_tag="ACG0BW_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFK7260884.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGAMGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPLTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(5982..6647)
                     /gene="mcbR"
                     /locus_tag="ACG0BW_003984"
     CDS             complement(5982..6647)
                     /gene="mcbR"
                     /locus_tag="ACG0BW_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFK7260885.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6845..7882)
                     /gene="curA"
                     /locus_tag="ACG0BW_003985"
     CDS             complement(6845..7882)
                     /gene="curA"
                     /locus_tag="ACG0BW_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFK7260886.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGIAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     VDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8063..8581
                     /gene="mddA"
                     /locus_tag="ACG0BW_003986"
     CDS             8063..8581
                     /gene="mddA"
                     /locus_tag="ACG0BW_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFK7260887.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8578..9027
                     /locus_tag="ACG0BW_003987"
     CDS             8578..9027
                     /locus_tag="ACG0BW_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFK7260888.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQSSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMEGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(9028..9261)
                     /gene="ydcY"
                     /locus_tag="ACG0BW_003988"
     CDS             complement(9028..9261)
                     /gene="ydcY"
                     /locus_tag="ACG0BW_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFK7260889.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9347..9520)
                     /gene="ortT"
                     /locus_tag="ACG0BW_003989"
     CDS             complement(9347..9520)
                     /gene="ortT"
                     /locus_tag="ACG0BW_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFK7260890.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9715..9810
                     /gene="yncL"
                     /locus_tag="ACG0BW_003990"
     CDS             9715..9810
                     /gene="yncL"
                     /locus_tag="ACG0BW_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFK7260891.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2439 a         2502 c         2505 g         2751 t
ORIGIN      
        1 taattacgcc agtgattcat taacattaac ctccggcatt attgttaaat aatgacattc
       61 ctttttaata cttcttgtaa tttcggtaac tgacatacag cttcagcgat cgcgctgata
      121 ttctgcgcat tatcgtgaaa ccattcatgc cccggccccc atgtgcgcat ggtgcagaga
      181 taacagtcca ccagcgttag ttgctcgcca aaggcataag gttcagcggt caattgtgaa
      241 ttcagccaga tataaagcga tttacgatat tcaataacat tattctttaa ctgttcgggt
      301 gcgtcaggag cccaacgctc agggtaatcg gcgaaagtga atgttggata gacattagca
      361 accagccaga ccaatagccg ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca
      421 aggtccgggc gacgatcgag aaccatcaat gcaatcgccg ctgtctccgt cataatctcg
      481 tcattttcca gcgccagtgt cggaatctga cacaacgggt tcagggtttt caataactcg
      541 cgtgaggttc cctcgtggtc aaaaccactg acatcaacga actgataagg aatatcagcc
      601 agtgttagca tcagctcact gattgtagag ccccatccgg gtacgccata caccttaatc
      661 atatgcccct cacaatcttt taaagattaa gtgtaggaca gtacacaggt taaccagcaa
      721 aaggatttgt cattttttcg tcagttgtat tattcccctt caggattgta aggggatgtt
      781 ttgcatcgtt tatcgataac gggtaggatg ctgcgccgat agttttatac ccataaagaa
      841 taatggtgat aactatcatc gccaggatga ataaacattg ttcatggcaa cttatatgac
      901 tttttcatta aagcaatcag ggagagcaat gagtaaacac gacaccgaca cttcagatca
      961 gcacgccgcg aaacgccgct ggcttaatgc ccacgaagag gggtatcaca aagcgatggg
     1021 caatcgccag gtacagatga tcgccattgg cggcgcgatt ggtacgggtt tgtttttagg
     1081 cgcgggagcc cgactgcaaa tggcggggcc agcactggca ctggtctatt taatctgcgg
     1141 cctgttttca ttttttattc tgcgtgcatt gggtgagctg gtgctacacc gcccttccag
     1201 tggcagtttt gtctcttatg cccgtgagtt tttaggagag aaagccgctt acgttgcagg
     1261 ctggatgtac ttcatcaact gggcgatgac ggggattgtc gatattaccg ccgtcgctct
     1321 gtatatgcat tactggggtg cgtttggcgg cgtgccgcag tgggtctttg cgctcgctgc
     1381 gctgaccatc gttggcacca tgaatatgat cggtgtgaaa tggtttgccg agatggagtt
     1441 ctggtttgcg cttattaaag tgctcgccat tgtgaccttt ttggtcgtgg gtacagtgtt
     1501 cctcggtagt ggtcagccgc tggatggcaa caccactggt tttcatttaa ttaccgataa
     1561 tggcggcttc ttcccccacg gtttgctgcc tgcgctggtg ttgattcagg gcgtagtgtt
     1621 tgcttttgcc tccattgaaa tggtgggtac agctgccgga gaatgtaaag atccgcagac
     1681 catggtgcct aaagcgatta acagtgtgat ttggcgtatt ggcctgtttt acgtcggctc
     1741 cgtggtgttg ctggttatgt tattgccgtg gagcgcatat caggcggggc aaagtccgtt
     1801 cgtgacgttt ttctctaaac tgggtgtgcc atatatcggc agcattatga acattgtggt
     1861 gctgaccgct gccctctcca gcctgaactc aggtctgtac tgcaccggac gtattctgcg
     1921 ctcaatggcg atgggcggtt ccgcaccgag ttttatggcg aaaatgagtc gtcagcatgt
     1981 gccgtatgcc gggattctgg cgacactggt tgtgtatgtc gtcggcgtat tcctcaacta
     2041 tctggtgccg tcgcgcgtat ttgagattgt gttgaacttc gcgtcgctgg gaatcatcgc
     2101 ctcatgggcg tttatcatcg tgtgccagat gcgcctgcgt aaagcgatta aagaaggcaa
     2161 agccgcggat gtgagtttta aactgcctgg cgcgcccttc acttcctggc tgacattact
     2221 gtttttgctg agcgtcctgg tgctgatggc gttcgattac ccgaacggga cttataccat
     2281 cgcggcgctg ccgattatcg gtattctgct ggttataggc tggtttggtg tgcgcaaacg
     2341 cgttgctgaa attcacagca ctgcgccagt cgtcgaggaa gatgaagaac aacaggaaat
     2401 tgtgtttaag cctgaaacgg cgagttaatt gcaaaattgc ccggctacgt gccgggcaaa
     2461 ttcaaattac tgacgaaggt accggatacg gcgtgataaa agtcaggcaa gtctctccgt
     2521 tttcaccctc gccatatcta ttacagcgca atacgaatca catcgtccgg ctgggtagct
     2581 tcctgctgtt tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac
     2641 agcgccaggc tgttcggatg agtcggcgta tcgaacgttt tcaccacttt atagcttttc
     2701 gcgtcaatca cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgcc
     2761 gggttaaaca gcacagccag tgattccggt gccgcaacct tcgccagaat attgccatta
     2821 cgggtatcga ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgcctg
     2881 gcggtatcaa ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta
     2941 cggctgagga ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg
     3001 gtgtaaagac gtttgccttt gctatccagc gccagaccgg tactcatttt accggtgttc
     3061 tggatggcgg ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta
     3121 ccaataccac tgatgtaaac ggtgttcgtg gcatcatcag ctaccagctc acgcggttgc
     3181 agcgggcgca cctcttccgt gcgcttacga tcatccagca ccagacggcc tttcacttcg
     3241 cccgttttgg catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacaacgtc
     3301 tgagtcgtgt tattgatggt ggcaccaaac ggcttgagat cgttatggat cgcctgcgtc
     3361 acttccagag taaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt
     3421 tgcgaagtgg cgagccacag cgcgttctcc tgctggctat aagccatttc gtaggcacct
     3481 ttacctaccg ctttacgcag catttcttct gcagcctgcg tactgaatga tgaagcaaca
     3541 agcaatgaac ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc
     3601 atgacgactc cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta
     3661 atcatcgtta cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag
     3721 catttcttgc cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt
     3781 ttccatatat ttacaatttt attaccatgg cggcgttttc ccattcttaa cctggctttt
     3841 ttcatgaaga ttttttccgt ccgacagacc gttttgcccg cactgcttgt cctttccccc
     3901 gttgtttttg ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca
     3961 gaactggata ccccagcagc cgtgagcgtg gtggatggcg aggagatgcg cctggcaaca
     4021 ccgcgcatta acttgtccga atcactgacc agcgtgcctg gtttgcaggt acaaaaccgg
     4081 cagaactatg cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac
     4141 ggaattcgcg gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacggacaa
     4201 gggcaaacat ccaacatcga tttaagcagt gtacaaaatg tggaagtgct gcgcggcccc
     4261 ttctctgctc tgtatggcaa cgcgtctggc ggtgtaatga atgtcatcac ccagaccgga
     4321 caacagccac caaccattga agccagtagt tactacggca gttttggcag ctggcgctat
     4381 gggctgaaag caacaggcgc aatgggcgac ggcactcagc ctggcgatgt cgattacacc
     4441 gtctcaacca cgcgctttac gacccacggc tatcgtgatc atagtggcgc acagaaaaat
     4501 ttagccaatg ccaaactggg cgtacgcatt gatgacgcca gcaaattaag cctgattttc
     4561 aatagtgtgg atatcaaagc agatgaccca ggtgggctaa ccgaagcaga atggaaggcg
     4621 aatccgcaac aagcgccacg cgctgaacag tacgacacgc gaaaaaccat caagcaaact
     4681 caggctgggt tgcgctatga acgtagcctg agcgcacaag atgatatgag tgtgatgatg
     4741 tatgccgggg agcgagaaac gacccagtac cagtcaatcc cgatggcacc acaacttaac
     4801 ccgtcgcatg cgggcggcgt gattactctg caacgccatt atcaggggat agacagccgc
     4861 tggacacacc gtggcgagct gggcgttccg ctcacgttca ctaccggcct gaactacgaa
     4921 aacatgagtg aaaatcgcaa aggctacaat aacttccgcc tgaatagcgg cgtgccggaa
     4981 tacgggcaaa aaggtgagtt gcgccgtgac gaacgcaatc tgatgtggaa cgtcgatccc
     5041 tatttgcaga cacagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac
     5101 agctccgtat ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc
     5161 ggtgatgcca gttatcacaa atggctacct gccggttcgt taaaatatgc aatgaccgat
     5221 gcctggaata tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg
     5281 tcttatcgtg ctgacggtca aagcggtatg aactttggtt taaaaccatc caccaacgat
     5341 acaattgaga tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttc
     5401 cagaccgaca ccgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac
     5461 aaaaatgccg gaaagacccg tcgtcaaggc gctgagctgg catgggatca acgtttcgcg
     5521 ggagattttc gtgtaaaagc gtcctggacc tggctcgatg cgacctatcg cagcaatgtt
     5581 tgcaatgaac aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt
     5641 gcgtcgatag gttatgtacc ggaggatggc tggtatgcag gtacggaagc gcgttatatg
     5701 ggcgatatta tggcagatga tgaaaatacg gccaaagcgc cgtcttatac tctcgtcggc
     5761 ttattcaccg ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc
     5821 gataatttat tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga
     5881 tattacgaac ctgcgcccgg gcgaaattat ggtgtcggca tgaatattgc atggcgattt
     5941 gagtaattac cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg
     6001 tataaaatag caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg
     6061 tcttttgctt ttaacgcggc gagtaataga cgatagtttt ctatacgctc ccgtaattcc
     6121 gctggattaa tcgcttcata gagataatgt aaaccaggtc ccatcctgac ccacagttgc
     6181 tcaatcatct cacacaggat gggcatattt gaacgatgat aaatcgctaa gcgaaatagc
     6241 ctgttcgcat taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg
     6301 agcaattcct cgagttccgc caggtcttgc ggggtgaggt tttcaacagc cagagtaact
     6361 gccattaatt ccagctcgta gcggatccga ttgatttcgt ctaattgacg tttccccact
     6421 tccggaactg taaatgcttg cgctggtgcg acagaaagcg cattcaccga aaccagacgt
     6481 aataatgctt cacgcacagg tgtaatactc atacctaatt gctccgccag atttttagta
     6541 atcaggcgtg cgccaggttt tagtgcgcca atactaagct gatgtttcag gtcattctca
     6601 acctgcagag tcaaactgac atgtttcatt ttttccgttc caggcatttt aattccttcg
     6661 cgtgttttct gtccaaccct gcctgcatgg cagaaggagt tcacctgggg tattaaagtg
     6721 aataatcata aaccactcac ctttgttgtt attagcagca gaacaatgac gtggttagtt
     6781 aattaaagct gaaaataagc tttttgcctg gatttttatg gcggcttacg ccgccgtcgt
     6841 atctttaatc atcacccgcc acgcggatca ccactttgcc gaagttttta cccttcagca
     6901 ggccgataaa tgtctgtggc gcattctcta aaccgtcagt aatttcttcg cggtagtgga
     6961 ttttatcctc tttcacccat tgccccatct ccttctgaaa ctcatggatg cggtgaccat
     7021 aatcctgagc gataataaat ccttgcaagc gaatacgttt tttcagcacc gtagccatca
     7081 gcagaggtaa acgatccggg ccgggtggta gctctgtagc gttatagctg ctcactaagc
     7141 cgcagacggg aatgcgcgca gatgtattaa gtaacggtag caccgcatca aatactttac
     7201 cgcccacgtt ttcataatag atatcaacgc cttttgggca cgctttcgcc agttgttcgg
     7261 caaaatcatc cgcgtgatgg tcaagacaaa catcaaagcc taacacctcg gtagcatggc
     7321 ggcatttttc cgcgccaccg gctatcccca ccaccctgca acctttaagt ttgccgattt
     7381 gacccaccgt cgcccccact ggtcctgtcg ccgcagctac caccagcgtt tcgccctctt
     7441 tgggctgacc gatatccagc agccccatat aagcggtaaa gcctggcatc cccagcacac
     7501 ccagcgacca cgatggattt tgcggatgat cgccaagttt caccagatca tcaccactgg
     7561 atatgtcata gtcctgccat ccactgtagc ccagcaccca gtcgccaggc tgataatcag
     7621 gatgattcga ctccacgaca cggctcaccg tgccgccgac catcacgccg ccaatatcaa
     7681 ccggcggtga ataagatggc tcatcgctca tacgtccacg catatacggg tccagtgaca
     7741 aataaactgt gcgcagtaac acctgtccct cacccggtgt ggcgatatca tcttcttcaa
     7801 gacggaaatt ctccggaacc ggcgcgccat gcggacgcga ggccagaacc caacgtcgat
     7861 tacgctgttt ttgttgcccc ataatgctct cctgtgcttt tggcatgaat cgatcaatag
     7921 tagcaggctc acgggattgg ttttgtttgc agccgtcaca tcccctctgc aacacgcaat
     7981 aatttcatcg aggaatccgt tgcttcgggc tgttttcgat agtattgata acgtcatcat
     8041 cattatttaa tcgaggccag acatgtccat ccgttttgcc cgcaaagccg actgtgctgc
     8101 cattgcggaa atttataacc acgccgtgtt gtatacggcg gctatctgga atgaccaaac
     8161 ggtggatgca gataaccgca ttgcctggtt tgaagcgcgg accatagcag gttatccggt
     8221 gctggtgagt gaagaagatg gcgtcgttac aggatatgcc tcgtttggcg actggcgtag
     8281 tttcgatggt tttcgccata ccgtggaaca ttcggtttat gtccatcccg atcatcaggg
     8341 caaaggtctg gggcgtaaat tgttaagccg attgattgat gaagcgcggg attgcgggaa
     8401 gcatgtcatg gtcgcgggga tcgaatcgca aaatcaggct tcgctgcatc tccaccagtc
     8461 gctgggattt gtcgtcaccg cgcaaatgcc gcaggtaggc actaaatttg ggcgctggct
     8521 ggatctgaca tttatgcagt tgcaactcga cgagcgcact gaaccggacg cgattggatg
     8581 aatcagtcgc ttacccttgc ttttttgatt gccgccggaa ttggtctggt ggtgcaaaac
     8641 acgctgatgg tgcgcatcac ccagagctcc tctaccattc tcatcgccat gttgctgaac
     8701 tctttggtag gaattgtgct gttcgtttct attttgtggt ttaaacaagg tatggagggg
     8761 tttggtgaac tggtgtccag cgtgcgctgg tggacactta tacctggctt gcttggatcg
     8821 ttttttgtct tcgccagtat cagcggatat cagaatgtgg gagcggcaac aactattgca
     8881 gtactggtcg ccagccagct gattggcggt ctggtgctgg atatcttccg cagccatggc
     8941 gtaccacttc gggcgttgat cggcccaatt tgtggcgcaa ttttacttgt ggttggcgcc
     9001 tggctggtgg ccagacgctc attttaatca gaggatggta ccgcctttgg ttaactgttc
     9061 gtggcgcgct tccatatctt ctttatgctt gcgaccatga tgagcaatag cggtgcgcaa
     9121 acgctgctgc tgggtatagc gatcttcccg acttaactcc gcgtcatcgc tcagggcgat
     9181 taataattcg ttcatatggg caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat
     9241 gacttcatcc agatgcgaca tactttctcc ttagccactt gtgaaattta tcgattcatc
     9301 caaatgatat aagtctgtgt ggtgccgtca agccagtgag taagatttaa aacagtgaaa
     9361 acaacagcgc caccgggaaa ctcatcggcc atgttgcccc caccagaaag gcgcttaaga
     9421 aacggatgcg tttcttatcg tgagaaagaa accaggtgat aagaaatgcg attgctgcca
     9481 taaccgcata aaaaacaagc atgtgttgat agagagacat gtacgggcgt ccagccagat
     9541 gaataaaacg cgcgcaaata tgcgtcattt tttgacgtgc atcaagtttt ttcaccagtt
     9601 tgagaaaaaa tcatcaaaca gcaacttgcc cttatccatc ttcagaatga gtattattgc
     9661 ggattttctt aacccgtact atacacatag gggcaatgat aaaaggtggc aaaaatgaat
     9721 gtttccagta gaactgtagt actgataaat gtctttgctg ctgttggttt gtttactctt
     9781 atctctatga gatttggctg gtttatttga tgtagtgatt ttgcacgcag cacgtactgt
     9841 cagagaaatc agtgctctga cagtacgcaa gatcatctca tactctccag gtttacaaca
     9901 ttatccttaa tatttacttc tttttcctga catctacatt gaaataagta gaaaacatcc
     9961 ccctcctgtc gtctatacag acagcttact gattcaaagg gaaccttgtt ttcacatttt
    10021 tctatcatca atactttaga cgcgcgggag tctataaatg ctttatactt tcagggaata
    10081 aagtaaatat aaaaagatgg cgtaaagtat atgcatggca ggatgattgc aacctccttg
    10141 atgacgctgt cccattgaaa ttgcaaaccc cgaactggtt atcttgccca tttatca
//
LOCUS       DAYPHN010000165        10183 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000165 DAYPHN010000000
VERSION     DAYPHN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.165"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            80..1474
                     /gene="galP"
                     /locus_tag="ACG0BW_003991"
     CDS             80..1474
                     /gene="galP"
                     /locus_tag="ACG0BW_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFK7260892.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1551..2048
                     /locus_tag="ACG0BW_003992"
     CDS             1551..2048
                     /locus_tag="ACG0BW_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFK7260893.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2143..2850
                     /gene="endA"
                     /locus_tag="ACG0BW_003993"
     CDS             2143..2850
                     /gene="endA"
                     /locus_tag="ACG0BW_003993"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFK7260894.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2930..3661
                     /gene="rsmE"
                     /locus_tag="ACG0BW_003994"
     CDS             2930..3661
                     /gene="rsmE"
                     /locus_tag="ACG0BW_003994"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFK7260895.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3674..4624
                     /gene="gshB"
                     /locus_tag="ACG0BW_003995"
     CDS             3674..4624
                     /gene="gshB"
                     /locus_tag="ACG0BW_003995"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFK7260896.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4733..5296
                     /locus_tag="ACG0BW_003996"
     CDS             4733..5296
                     /locus_tag="ACG0BW_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFK7260897.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5296..5712
                     /gene="ruvX"
                     /locus_tag="ACG0BW_003997"
     CDS             5296..5712
                     /gene="ruvX"
                     /locus_tag="ACG0BW_003997"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFK7260898.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDDTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5827..6807)
                     /locus_tag="ACG0BW_003998"
     CDS             complement(5827..6807)
                     /locus_tag="ACG0BW_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFK7260899.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6825..7529
                     /gene="yggS"
                     /locus_tag="ACG0BW_003999"
     CDS             6825..7529
                     /gene="yggS"
                     /locus_tag="ACG0BW_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFK7260900.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7547..8113
                     /gene="yggT"
                     /locus_tag="ACG0BW_004000"
     CDS             7547..8113
                     /gene="yggT"
                     /locus_tag="ACG0BW_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFK7260901.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8110..8400
                     /gene="yggU"
                     /locus_tag="ACG0BW_004001"
     CDS             8110..8400
                     /gene="yggU"
                     /locus_tag="ACG0BW_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFK7260902.1"
                     /translation="MSAVTVNDGGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8408..9001
                     /locus_tag="ACG0BW_004002"
     CDS             8408..9001
                     /locus_tag="ACG0BW_004002"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFK7260903.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            8994..10130
                     /gene="hemW"
                     /locus_tag="ACG0BW_004003"
     CDS             8994..10130
                     /gene="hemW"
                     /locus_tag="ACG0BW_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFK7260904.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2377 a         2695 c         2697 g         2414 t
ORIGIN      
        1 tgtgatttgc ttcacatctt tttacgtcgt actcacctat cttaattcac aataaaaaat
       61 aaccatattg gagggcatca tgcctgacgc taaaaaacag gggcggtcaa acaaggcaat
      121 gacgtttttc gtctgcttcc ttgccgctct ggcgggatta ctctttggcc tggatatcgg
      181 tgtaattgct ggcgcactgc cgtttattgc agatgaattc cagattactt cgcacacgca
      241 agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg gcagcggctg
      301 gctctccttt aaactcgggc gcaaaaagag cctgatgatc ggcgcaatcc tgtttgttgc
      361 cggttcactg ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt cccgcgttct
      421 gctggggctg gcggtgggtg tggcctctta taccgcaccg ctgtacctct ctgaaattgc
      481 gccggaaaaa attcgcggca gtatgatctc gatgtatcag ttgatgatca ctattgggat
      541 cctcggtgct tatctttctg ataccgcctt cagctacacc ggtgcatggc gctggatgct
      601 gggtgtgatt atcatcccgg caattttgct gctgattggt gtcttcttcc tgccagacag
      661 cccacgttgg tttgctgcca aacgccgttt tgttgatgcc gaacgcgtgc tgctgcgcct
      721 gcgtgacacc agcgcggaag cgaaacgtga actggatgaa atccgtgaaa gtttgcaggt
      781 taaacagagt ggctgggcgc tgtttaaaga gaacagcaac ttccgccgcg cggtgttcct
      841 tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg aacgtcatca tgtattacgc
      901 gccgaaaatc ttcgaactgg cgggttatac caacaccacc gagcaaatgt gggggacggt
      961 gattgtcggc ctgaccaacg tacttgccac ctttatcgca atcggccttg ttgaccgctg
     1021 gggacgcaaa ccaacgctaa cgctgggctt cctggtgatg gctgctggca tgggcgtact
     1081 cggtacaatg atgcatatcg gtatccactc tccgtcggcg cagtatttcg ctatcgccat
     1141 gctgctgatg tttattgttg gctttgccat gagtgccggt ccgctgattt gggtactgtg
     1201 ctccgaaatt cagccgctga aaggccgcga ttttggcatc acctgctcca ccgccaccaa
     1261 ctggattgcc aacatgatcg ttggcgcaac gttcctgacc atgctcaaca cgctgggtaa
     1321 cgccaacacc ttctgggtgt atgcggctct gaacgtactg tttatcctgc tgacattgtg
     1381 gctggtaccg gaaaccaaac acgtttcgct ggaacatatt gaacgtaatc tgatgaaagg
     1441 tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct caggcttcct cccgtcgcgg
     1501 aggaagccgc ctcttgcagt catcttttct tcgctctatc ctctgccgct atgaaaacat
     1561 cccgtctccc tatcgccatc caacaggccg ttatgcgtcg cctgcgggaa aaactcgccc
     1621 aggccaacct gaagctaggg cgtaactacc cggagccaaa actctcttac acccagcgcg
     1681 gaacctccgc cggaacagcc tggctggaaa gctatgaaat ccgcctcaat cccgttttgc
     1741 tgttggaaaa cagtgaagcc tttattgaag aagtggtgcc gcacgaactg gcacatttgc
     1801 tggtatggaa acatttcggc cgcgtagcgc cacatggcaa agagtggaag tggatgatgg
     1861 aaaacgtgct gggtgttccc gcccgtcgta cgcatcagtt cgaactgcaa tccgtgcgtc
     1921 gcaacacctt cccctaccgc tgtaagtgcc aggaacatca gcttaccgta cgccgccata
     1981 atcgcgtagt tcgtggcgag gccgtctatc ggtgtgttca ctgcggtgaa cagctggttg
     2041 cgaaataacc acctgaacta tcaggaactt tcctgatctg gctgattgca taccaaaaca
     2101 actttcgcta cgttgctggc tcgttttaac acggagtaag tgatgtaccg ttatttgtct
     2161 attgctgcgg tggtactgag cgcagcattt tccggcccgg cgttggccga aggtatcaat
     2221 agtttttctc aggcgaaagc cgcggcggta aaagtccacg ctgacgcgcc cggtacgttt
     2281 tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg ttgttgatct gcaatcgtgc
     2341 ggctatcagg tgcgcaaaaa tgaaaaccgc gccagccgcg tagagtggga acacgtcgtt
     2401 cccgcctggc aattcggtca ccagcgccag tgctggcagg acggtggacg taaaaactgc
     2461 gccaaagatc cggtctatcg caagatggaa agcgatatgc ataacctgca accgtcagtc
     2521 ggcgaggtga atggcgatcg cggcaacttt atgtacagcc agtggaatgg cggcgaaggc
     2581 cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa aagccgccga accaccagcg
     2641 cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc gtgaccaata caacctgaca
     2701 ctctctcgcc agcaaacgca actgttcaac gcatgggaca agatgtatcc ggttaccgac
     2761 tgggagtgcg agcgcgatga acgcatcgcg aaggtgcagg gcaatcataa cccgtatgtg
     2821 caacgcgctt gccaggcgcg aaagagctaa cctacactag cgggattctt tttgttaacc
     2881 cctaccccac gcgtacaacc gcgtggggag acgacgcgga tttttaacta tgcgtatccc
     2941 ccgcatttat catcctgaac cactgaccag ccattctcac atcgcgcttt gcgaagatgc
     3001 cgccaaccat atcgggcgcg tactgcgtat ggggccggga caggcgttgc aattgtttga
     3061 cggtagcaac caggtctttg acgccgaaat taccagcgcc agcaaaaaaa gcgtggaagt
     3121 gaaggtgctg gaaggccaga tcgacgatcg cgaatctccg ctgcatattc acctcggtca
     3181 ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag aaatcgatcg aactcggtgt
     3241 aagcctcatt acgccacttt tttctgagcg ctgcggcgtt aaactggata gtgaacgtct
     3301 gaacaagaag ctccagcagt ggcagaagat tgcaattgct gcctgtgagc agtgtggtcg
     3361 taaccgggtg ccggaaatcc gtccagcgat ggatctggaa gcctggtgtg cagagcagga
     3421 tgaaggactg aaactgaatc ttcacccgcg cgccagtaac agcatcaata cgttgccgtt
     3481 accggttgaa cgcgtccgcc tgctgattgg cccggaaggc ggtttatcgg cagatgaaat
     3541 tgccatgact gcccgctatc aatttactga tatcctgttg ggacctcgcg ttttgcgtac
     3601 agagacaact gcgctcaccg ccattaccgc gctacaagta cgatttggcg atttgggcta
     3661 acggagaaga ataatgatca agctcggcat cgtgatggac cccatcgcaa acatcaacat
     3721 caagaaagat tccagtttcg ctatgttgct ggaagcacag cgtcgtggtt atgaacttca
     3781 ctatatggag atgggcgatc tgtatctgat caatggcgaa gcccgcgccc atacccgcac
     3841 gctgaacgtg aagcagaact acgaagagtg gttttcattc gtcggtgaac aggatctgcc
     3901 gctggccgat ctcgatgtga tcctgatgcg taaagacccg ccgtttgata ccgagtttat
     3961 ctacgcgacc tatattctgg aacgtgccga agagaaaggg acgctgatcg ttaacaagcc
     4021 gcagagcctg cgcgactgta acgagaaact gtttaccgcc tggttctctg acttaacgcc
     4081 agaaacgctg gttacgcgca ataaagcaca gctgaaagcg ttctgggaga aacacagcga
     4141 catcattctt aagccgcttg acggtatggg cggcgcgtcg attttccgcg tgaaagaagg
     4201 cgatccaaac ctcggcgtga ttgccgaaac cctgactgag catggcactc gctactgcat
     4261 ggcgcaaaat tacctgccag ccattaaaga tggcgacaaa cgcgtgctgg tggtagatgg
     4321 cgagccggta ccgtactgcc tggcgcgtat tccgcagggg ggcgaaaccc gtggcaatct
     4381 ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa agtgactgga aaatcgcccg
     4441 tcagatcggg ccgacgctga aagaaaaagg gctgattttt gttggtctgg atattatcgg
     4501 cgaccgtctg actgaaatta acgtcaccag cccaacctgt attcgtgaga ttgaagcaga
     4561 gtttccggtg tcgatcaccg gaatgttaat ggatgccatc gaagcacgtt tacagcagca
     4621 gtaacccacc ttagcgagaa ggatctcgtt gagactctga gtgacagcgc ccttctttcc
     4681 acgcatactg ggcgctgttg cttttttgaa ccaggaaaca gaacctctga caatgaattt
     4741 acagcatcac tttcttattg ccatgcctgc tctccaggat ccgattttcc gtcgttccgt
     4801 ggtctacatt tgcgaacata ataccaatgg tgcaatgggg atcatcgtca acaagccgct
     4861 ggaaaatctc aaaattgaag ggattctgga aaagctgaag atcacgccgg agccgcgtga
     4921 tgaatcaatc cgtctggata aaccggttat gctcggcggt ccgctggctg aagatcgcgg
     4981 gtttattttg catactccgc cctctaattt tgcttccagc attcgcattt cagacaacac
     5041 ggtgatgacc acttcccgcg atgtgctgga aacgctcggc accgataaac aaccgtctga
     5101 cgtattggtg gctctgggct atgcctcctg ggagaaaggt caactggaac aagaaattct
     5161 cgataacgcg tggctaacgg ccccggcgga tctgaatatt ctgttcaaaa caccgattgc
     5221 cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat attctcacca tgcctggtgt
     5281 ggcagggcac gcctgatgag tggaacctta ctcgctttcg acttcggcac caaaagcatt
     5341 ggtgtagcgg tcggccaacg cattaccggc accgctcgcc cattgcctgc aattaaagca
     5401 caggacggta cgccggactg gaatctcatc gaacgtttac tgaaagagtg gcagccggac
     5461 gaaatcatcg tcggtttgcc gctgaatatg gacgacaccg aacagccgtt aaccgccaga
     5521 gcgcgtaaat ttgccaaccg tattcatggc cgtttcggtg ttgaagtaaa gctccatgac
     5581 gagcgtctta gcactgtgga agcccgttcc ggtctgtttg aacagggcgg ctatcgggcg
     5641 ctcaacaaag gcaaagttga ctctgcctct gcggttatca ttctcgaaag ttatttcgag
     5701 cagggatatt aaggcgattt acgtcatcgc tccttattgc cggatgcggc gtgaatgact
     5761 acatttgtaa gcctgataag cgtagcgcat caggcatatt tacgaagcta atcaatcact
     5821 ttagcctcac aaacgtcctt cccccacccg ctgctgataa ctctgctgaa acgttaacat
     5881 ccccacctgc tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat
     5941 caaattcccc accgcgggtg tgttaaccag caattcaaac agcgccacgc gtccttcctg
     6001 tttatccact tccagctttt gtgacaacac cgcccgtaaa ctacctgcca gttgattacg
     6061 cacgggatct ttttcctgcg ctggaaatga atccaccagt cgctcaactg cctgcgccgc
     6121 accacgtgta tgtaaagttg ccagcaccag gtgtccggtt tctgccgccg tcagcgccag
     6181 acggattgtc tcgctgtcac gtagctctcc tagcaaaatc acatcaggat cttcccgcaa
     6241 cgcagcccgc aatcccgatg cgaacgtcat acagtgcaaa ccaatctccc gctgctggat
     6301 caaacatcgc tggctggcat agagatattc aacaggatct tccagcgtca gaatatgcgc
     6361 atcggcatgt tgattgagat agccaaccat cgccgccagc gtggtagatt tgccgctccc
     6421 cgtcgccccc gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg
     6481 tggcgcacca agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga
     6541 aataccttgc cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac
     6601 agcaaaatcc aactgaccat tctccagcaa tattgtccgc tgatcgtcat ccagccactc
     6661 ccgcagtagc tcttcgacgt ccggcgcatc aaacggcgca gcttccattc gcccgcgaat
     6721 gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact
     6781 aagggccaca atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc
     6841 ataacctggc acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc
     6901 cagaagaaat tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag
     6961 ccattgatgc cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa
     7021 ttcgccactt tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc
     7081 agtctaataa aagccgcctg gtggcagagc atttcgactg gtgtcatacc atcgaccgtt
     7141 tgcgcatcgc tacccgcctg aacgaccagc gcccggcaga acttccccct cttaacgtac
     7201 tgattcaaat taacattagt gatgaaaaca gtaagtccgg gattcaactg gctgaactgg
     7261 acgagctggc agctgcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa
     7321 tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag
     7381 catttgccgg actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg
     7441 acgatatgga agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt
     7501 ttggtgcgcg tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac
     7561 tttcctgctt tcaacggtca ttgagctgta taccatggtg ctgttattac gcatctggat
     7621 gcagtgggct cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtaacgca
     7681 gccaattatc gggccactgc gccgcgttat tccggcaatg gggcccattg acagcgcctc
     7741 actgctggtt gcgtacattc tcagtttcat caaagccatc gtgctgttta aagtggtgac
     7801 cttcctgcca atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcctgct
     7861 gattttctgg gtcctgctgg tgatggcgat tatgagctgg gtaagccagg ggcgtagccc
     7921 gattgaatac gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcctgct
     7981 accggcaatg ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgtcat
     8041 caatatgggt gtcgcagaag tcttgcaggc gaccggaaat atgctgctgc cggggctgtg
     8101 gatggcgtta tgagtgccgt aacagtgaat gatggcggtc tggttttacg gctctatatt
     8161 cagccgaaag ccagccgtga ttctattgtc ggtttacatg gcgacgaggt aaaagtcgcc
     8221 attaccgcgc cgccggttga cggccaggcc aacagccatc tggtgaagtt tctcggtaag
     8281 caattccggg ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa
     8341 caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag
     8401 gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc
     8461 gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa acagacctcg gtgtagattc
     8521 cgctgaagaa actggcctga cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc
     8581 aaaagtgacc ggtttaccgg caattgccga cgactccggt ctggcggtag atgtacttgg
     8641 cggtgcaccg gggatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa
     8701 tctgcaaaaa ctgctggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt
     8761 ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tgtgccacgg
     8821 cagctggccg ggcgtgatta ctcgtgaacc tgcgggtact ggtggctttg gttatgatcc
     8881 aattttcttc gttccttccg aagggaaaac cgctgccgaa ctgacccgcg aagaaaagag
     8941 cgccatttcc caccgtggtc aggcattgaa actgctgctg gacgctttac gtaatggtta
     9001 aattaccgcc gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact
     9061 gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc
     9121 tgctttgcga tctggacaac gacgtggctt acgctcaggg ccgtgaagtg cagacaatct
     9181 ttattggcgg tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctggacg
     9241 gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag
     9301 gcacggtaga agcggatcgc tttgtcgatt atcagcgtgc tggtgtgaac cgcatctcta
     9361 ttggtgtgca gagttttagc gaagaaaagc tgaaacgact cgggcgtatt catggcccgc
     9421 aagaagcgaa acgagcggca aagctggcga gcggtttagg gttacgtagt tttaaccttg
     9481 atttgatgca tgggctgccg gatcaatcac tggaagaggc gctaggcgat ctgcgccagg
     9541 ctattgaact gaatccgcca catctttcct ggtatcaact gaccatcgaa cctaatacgc
     9601 tgtttggctc gcgccctcct gtactgccgg acgatgacgc gctgtgggat attttcgaac
     9661 aggggcatca gttattaacc gcagcgggct atcagcagta tgaaacatcc gcttacgcca
     9721 aacccggtta tcagtgccag cacaatctca actactggcg atttggcgac tacatcggta
     9781 ttggctgcgg cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacca
     9841 aaacgcgtca tccgcgtggt tttatgcagg gaagatatct ggaaagccag cgtgatgtcg
     9901 aagctgcaga taaaccgttt gagttcttta tgaatcgctt ccgtctgctg gaagccgcgc
     9961 cgcgcgtgga gtttagccag tatactggcc tttctgaaga agttattcgc tctcagttag
    10021 acgaggctat cgctcagggt tatctcacag aatgtgcgga ttactggcag ataaccgagc
    10081 atggtaaatt attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc
    10141 gcctgcgggc gcattaatca cttcttttac cccccgttaa tta
//
LOCUS       DAYPHN010000166        10159 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000166 DAYPHN010000000
VERSION     DAYPHN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.166"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..138)
                     /locus_tag="ACG0BW_004004"
     CDS             complement(<1..138)
                     /locus_tag="ACG0BW_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260905.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EF"
     gene            216..1310
                     /gene="ddlA"
                     /locus_tag="ACG0BW_004005"
     CDS             216..1310
                     /gene="ddlA"
                     /locus_tag="ACG0BW_004005"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFK7260906.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDTQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1513..1725)
                     /gene="yaiZ"
                     /locus_tag="ACG0BW_004006"
     CDS             complement(1513..1725)
                     /gene="yaiZ"
                     /locus_tag="ACG0BW_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFK7260907.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1985..2293
                     /gene="yaiY"
                     /locus_tag="ACG0BW_004007"
     CDS             1985..2293
                     /gene="yaiY"
                     /locus_tag="ACG0BW_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFK7260908.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2351..3445)
                     /gene="yaiW"
                     /locus_tag="ACG0BW_004008"
     CDS             complement(2351..3445)
                     /gene="yaiW"
                     /locus_tag="ACG0BW_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFK7260909.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3458..4678)
                     /gene="sbmA"
                     /locus_tag="ACG0BW_004009"
     CDS             complement(3458..4678)
                     /gene="sbmA"
                     /locus_tag="ACG0BW_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFK7260910.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            5030..6187
                     /gene="ampH"
                     /locus_tag="ACG0BW_004010"
     CDS             5030..6187
                     /gene="ampH"
                     /locus_tag="ACG0BW_004010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFK7260911.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADYIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6188..6811)
                     /gene="iprA"
                     /locus_tag="ACG0BW_004011"
     CDS             complement(6188..6811)
                     /gene="iprA"
                     /locus_tag="ACG0BW_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFK7260912.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6897..9803)
                     /locus_tag="ACG0BW_004012"
     CDS             complement(6897..9803)
                     /locus_tag="ACG0BW_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFK7260913.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPVTALD
                     WDHMDKAGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYADSTAN
                     QLDISNSVIHGSITSLKPGGYYDRLDADSDLLVGYDFYTDAFVDTNWRDGDVFTLNIA
                     NTTIDDDYEAFYFTDSYKDGDVTKHTNETFDTSEGVAVNLDVESNINISNNSRIAGIA
                     LSQGNTYNETYTTESHTWDNNISVKDSTVTSGSDIILDSDTYVKTGHFGNSSEPSDYT
                     GPGDVAMSFTDSGSDYAMKNNVYFSNSTLMGDVAFNSTWNSNFDPNGHDSNGDGLKDT
                     NGGWTDDGLNVDELNLTLDNGSKWVGQAIYNVAETSAMYDVATNSLTPNATYENNDWK
                     RVVDDKVFQSGVFNVALNNGSEWDTTGRSIVDTLTVNNASQVNVSESKLTSDTIDLTN
                     GSSLNIGEDGYVDTDHLTINSYSTVALTESTGWGADYNLYANTITVTNGGVLDVNVDQ
                     FDTEAFRTDKLELTSGNIADNNGNVVSGVFDIHSSDYVLNADLVNDRTWDTAQANYGY
                     GIVAMNSDGHLTINGNGDINNGDELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKD
                     KEIIYVNDVNTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSAN
                     TNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVD
                     TKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYS
                     HFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDD
                     YQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNG
                     DSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2683 a         2497 c         2357 g         2622 t
ORIGIN      
        1 aaactcactg catggccata caagccagga ataaattagc ttcccgttca tcatttttgt
       61 gatcttgcgc acatcattga gataacgcac tttcttcacc agtacgttgc acgctttgtc
      121 agcagaagat tgcaacattc tgtcagaagg ccgcgaatcc cccgttttgt cgtggtaatg
      181 tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg
      241 tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg
      301 atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa caagggcaat
      361 ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcat
      421 tgcgcccttc ggcgaccagt cttgcgcagg tgcctggtaa acatgaacat cagcttatcg
      481 acacgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta
      541 cgctgggcga agatggttct ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag
      601 gttctgatgt tctggcttca gcggcctgta tggataaaga tgtcaccaaa cgtctgctgc
      661 gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca
      721 tcagttttgc cgaagtggag tctaaactgg ggttaccgct gtttgtaaaa ccggctaatc
      781 agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca attgccgtcg
      841 atctggcgtt cgagttcgac cataaagtga tcgttgagca agggatcaaa ggtcgtgaga
      901 tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc gagatcgtac
      961 tcaccagcga tttctatgcc tacgacacca agtacattga tgaagatggc gcgaaagtgg
     1021 tagttccggc agccattgcg ccagaaatca acgataagat ccgggcgatt gccgttcagg
     1081 cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga
     1141 acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc agtatgtatc
     1201 cgaagctgtg gcaagccagc ggtctgggtt acaccgacct gatcacccgt ttgattgaac
     1261 tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa tttttaaatg
     1321 cctgataact tttattgggc atttaagact gatggaaaaa gtaagattgc ctgatgcgct
     1381 ttgcttatca ggcctaccag ataattgcaa tatactgatt ttgcatgatt ttgtaggcag
     1441 ggtaaggcgt tcgcgccgca tccggcaaaa acaacgagca ctttgtcagc ccccagaacg
     1501 cccaatcctc tgttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcagccc
     1561 cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt
     1621 tgcttcaaat cccagtaaac ccaccacgcc attaagaatg aatataaggc caatggcgaa
     1681 cgcatagtag tgccagtcac ggcggatttt tacaggcaag ttcatggcag ctccgttaac
     1741 gaaaaacaac atccttgcat attctgcaac aaccgtctgt ggcgtagaca acacttccgt
     1801 aattgttaat aaatttaact taaggttatc attttgtgac tcacgacgaa gattcacgct
     1861 tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct aaatgtcctg
     1921 tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt
     1981 ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc
     2041 ttcaacgcct ggcaatattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc
     2101 gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca
     2161 ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat
     2221 cccgttttta gtaggctgcc tcatttttgc agtagtggcg ctatggctgc actggcgaca
     2281 tcgtcgccag taagatttac ttgccagata gggcctaaaa cgccttatct ggcatgacat
     2341 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2401 aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2461 cgcggggtag tgatttcccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2521 ccgtctcctc aaaagagaag ctgtcgcctt tctctaactg gcggcgaatt tcactgtcgc
     2581 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggctctttac
     2641 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgaccg
     2701 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaaacggt
     2761 atatcggcgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2821 cgccgcgacg actgaagact tcctgacgga ctgtaccgtc cattttccac ggataccctt
     2881 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat
     2941 taagcgaacc aaacaacgtc tgccccattg gcaccatgct gatcagatcg tcaaaaatcg
     3001 cgctcaattg cttttctgta cgcaccgaat ctaaccgctc gctatagctt ttgccgtttg
     3061 gcgatttgat ttttagcgcg gtatgcacca gaaaagcagg aatgtgcatc cgttcagcac
     3121 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3181 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3241 gcccctgact ttcaaacgtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3301 tcgcaggcat cttctgccgc acgacgctcg ccacatctat cgctttttcg ccctttttca
     3361 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3421 aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3481 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     3541 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagctccg cgaacctgac
     3601 caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg
     3661 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt
     3721 tgaaatacat atagtgaaaa tagaggcgga aatagttttt ccgtacggcg ctaaacagct
     3781 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac
     3841 ggtaggcagc ctctacacgc tggtttttaa actccagccc cggcagtttg atccctacca
     3901 ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc aggccatacg
     3961 gaatgtgccc gacaatcggc agctccggca catgcgcgga gagcgttacc agcaccggca
     4021 ggaaggcgat caacgtcatg atagcgttga taaagctgac gcccatattc tccagcgttg
     4081 aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt
     4141 gttgccagtt cgccatgtaa tattcgttca tcgctgtacg ccagcggaac acgtagtgac
     4201 tgacaaagaa gttgttcagc acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     4261 cgcccacttc gcggtaaaat tgttcgatgg tgactttatg cggcgaactt agcgcggttt
     4321 gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact tccaccaaaa
     4381 accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc caacgatgcg
     4441 ggctgtagat aaaccagaac aatgcaaaaa gtcctacaca aacaatgtag taagcgtaaa
     4501 aaatcagaaa atccaacgac cagaaacgcg cggcgctaat cgggatttgc ccggaagctc
     4561 cggtgatacg cgccacccag tcacccccac ctgcttgcca gaagataacg gcaatcaatg
     4621 cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat
     4681 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     4741 caaagagtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     4801 gcctgagcat aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg
     4861 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4921 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4981 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     5041 tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc
     5101 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatt atatttttta
     5161 cggcagcggt gccacgggga tggcgctggt agttatcgac ggtaaccagc gcgtctttcg
     5221 tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcatacg
     5281 tattgcttcc ctcaccaagt taatgaccag tgagatgctg gtgaaattgc tcgatcaggg
     5341 gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtaccgac
     5401 ctacaatggg acgcccatta cgctagtgaa tctggcaacc cataccagcg ccctgccccg
     5461 tgaacagccc ggtggcgcgg cacatcgtcc ggtatttgtc tggccaacgc gcgagcaacg
     5521 ctggaaatac ctttccactg ccaaactaaa agccgcgccg ggcagccagg ccgcgtactc
     5581 caaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac
     5641 ccagttgttt gaagagcaga ttacccgccc attggggatg aaagacacca cctacacccc
     5701 ctcgccggac cagtgccgcc gtttaatggt tgctgagcgt ggtgccagtc cgtgcaataa
     5761 cacgctggcg gcaattggca gcggcggagt ctattccacg cctggcgata tgatgcgctg
     5821 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca
     5881 gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa
     5941 agcggacgcg cttggtttag gctgggtata tatggccccg aaagagggtc gtccagggat
     6001 tattcagaag acaggtggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     6061 gaacattggc gcatttgtgg tggtcactcg ttcgccattg acgcgcttta aaaatatgag
     6121 cgatggcatc aatgacctgg tgaccgaact gagcgggaat aaaccgttgg tcatccccgc
     6181 atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6241 cgatatagcc gcctttacgt aaagcagcga gaacttctgc gacgacagaa cgcgatatgc
     6301 gtgtacgttg atggatgtaa ttcatcacac caatacgtga gcgtaattct tcattccagt
     6361 caatcattga taataacgtt gcgcggattt gttcgtagga attatgccca atgagctgta
     6421 cgtcacgtaa ttccagaatt ctattttgcc aggctaacca ataaaaagcg tctcgccaga
     6481 gttgattttg ttcaataagg gtaatggttt gtttggctgg tagatgatat cccgtacaat
     6541 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagtccca
     6601 taatataagg agcctgggta ataccgataa gaacgttttc ttctctacgc agagagataa
     6661 cgccctctaa aataacgaaa gtatcttcgc tattgacatc acttgaaaat ataacttgct
     6721 tttcattatt aaactcgaag cgcgtgccat atctggacaa acatttatcg agcttaccaa
     6781 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     6841 aatccttgac gggcattatt tagattaaat aacctatttc ttcagagtga agaatattac
     6901 caggtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc accaccgagg
     6961 tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca
     7021 acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg
     7081 ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg
     7141 ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac
     7201 tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg gaacagacca
     7261 gaaacgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc gtaaccggct
     7321 ttcaaaccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt accgttgctc
     7381 atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccatc aacaaagacg
     7441 ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg atccacctga
     7501 ccagaacggt cattcatgtc acctttagcg aagcctgcag ccgcaccgac gatccactta
     7561 gcgttgttac cgtcaatttt ggtatccaca ccgaccatga tgccgttaac atcctgatca
     7621 tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg
     7681 ccgttatcag ccaggccatg acgagagttg gtcagacgag tgccaacggt gtcttgttcc
     7741 aggttccaga tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc
     7801 agctccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata ggtgtatgca
     7861 cccaagtcag ctttgttagc agcagagaag gttgcgttgg tgttgacgtc gtttacgtag
     7921 ataatttctt tatctttgta atcagcgatt gcgccagcgc cagttgcgtt gtcgatacga
     7981 actttgtagt taccggttgc agcaacaacg ttgtctacag aactgttatc cagttcgtcg
     8041 ccgttgttga tgtcgccgtt accattgata gtcaggtgac catcagagtt catagcaaca
     8101 ataccgtagc cgtagttagc ctgagcagtg tcccaggtac ggtcgttcac caaatccgcg
     8161 ttcagaacgt aatcgctgct gtggatatcg aatacaccag acactacgtt gccgttattg
     8221 tcagcaatgt tgccgctggt cagttccagt ttgtcagtac ggaaagcttc agtatcgaac
     8281 tgatcaacgt tcacatccaa tacaccaccg ttagttacgg tgatggtatt ggcgtacagg
     8341 ttgtaatcag caccccaacc tgtagattcg gtcaacgcaa cagtactgta ggagttaata
     8401 gtcagatgat cggtatcaac gtaaccatct tcaccaatgt tcagcgaaga accgttagtt
     8461 aaatcgatag tatctgaagt taatttagat tccgaaacat taacctgaga agcattatta
     8521 actgtcaagg tatcaacgat ggaacgacct gtagtatccc attcagaacc gttgttcaac
     8581 gctacgttaa atacaccgct ctggaagacc ttgtcatcaa caacacgttt ccagtcattg
     8641 ttttcatatg ttgcattagg agtaaggctg tttgtagcaa catcatacat tgctgacgtt
     8701 tcagcaacgt tataaattgc ctgaccaacc cacttgcttc cgttatcaag agtgaggttc
     8761 agttcatcaa cgttgaggcc atcatcagtc caaccaccgt tggtatcttt cagcccgtca
     8821 ccgttggaat catgaccatt cggatcaaaa ttactattcc aagtgctgtt aaatgcaaca
     8881 tcgcccatca gcgttgaatt gctgaagtat acgttgttct tcattgcata gtcggaacct
     8941 gaatcagtga aggacattgc aacatctccc ggaccggtat aatcactcgg ttcactagag
     9001 ttgccaaagt gtcctgtttt gacataagta tcgctatcca gaataatgtc tgaaccagaa
     9061 gtcactgtgg aatcttttac agagatattg ttatcccaag tatgagattc ggtagtataa
     9121 gtttcgttgt aagtattacc ttgagataat gcaatacctg caatgcggga gttatttgaa
     9181 atattgatgt tactttctac atcaagatta acagcaacac cttcactggt atcaaatgtc
     9241 tcatttgtgt gtttggttac atcaccatct ttataagaat cagtgaagta aaacgcttca
     9301 taatcatcat caatggtagt gttagcaatg ttaagagtga aaacatcacc atcacgccag
     9361 tttgtgtcaa caaaagcatc agtgtaaaaa tcatatccaa ccagaagatc actatctgca
     9421 tccaaacgat cataataacc gcctggcttc aaagaagtaa tcgaaccgtg aatcactgaa
     9481 ttactgatat caagctgatt agcagtactg tctgcataat catggttcaa ataataggtt
     9541 gagatcgtgc cgttaacggt atcgccgtta aaggactgat cgaaatcacc attggtgttg
     9601 ttgtaataga caaaaccact atagtttaca tagttgccat ctacagtgcc tgccttatcc
     9661 atatgatccc aatcaagtgc agtaacggga ttgtcgtaat aagtccaggt gtcataagta
     9721 gttgcattag cctgagaggt gatagccaga gtgcaagcca atgctaattg tgatactaca
     9781 agtttctttt tccaggagtg catttgtcat ccctcctcag ggacgtaata agttgatcca
     9841 tcaattatca atgcatagaa aacatcttat gtttcgcgtt gacgattatg cggcgttata
     9901 gaggaatggt tcaattattc tttccccgaa gtccggttac ggacatatat aaagcacgga
     9961 ggaacacaca aagaaaaaat tgataaaggc ttgatgtttc aacaagttaa caggagttcc
    10021 atttttaaag catatgcatt taaaatatca aacacacaaa ccagacaaat aatctatcaa
    10081 taaccaatga actttaaatc attctgtttt tgataacgca atcaaatccg taaaaacaat
    10141 aatccaggga tataaataa
//
LOCUS       DAYPHN010000167        10149 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000167 DAYPHN010000000
VERSION     DAYPHN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.167"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..4377
                     /gene="irp1"
                     /locus_tag="ACG0BW_004013"
     CDS             <1..4377
                     /gene="irp1"
                     /locus_tag="ACG0BW_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104999970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFK7260914.1"
                     /translation="DGLAQQFSTLDAPKTLSVAWGAWGESGRAATPEMLATLASRGMG
                     ALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPLQQALFNISATEKAATPVIPPADD
                     NAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHPNQDLLQLGMDSLLFLELSSDIQH
                     YLGVRINAERAWQDLSPHGLTQLICSKPEATPAASQPEVLRHDADERYAPFPLTPIQH
                     AYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAILEKAWNQLIARHDMLRMVVDADGQ
                     QRILATTPEYHIPRDDLRALSPEEQRIALEKRRHELSYRVLPADQWPLFELVVSEIDD
                     CHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETLAPLAITFRDYVMAEQARRQTSAW
                     HDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTFKSTIGKTEWQAVKQRWQQQGVTP
                     SAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHPQINQLIGDFTSVTLVDFNFSAPV
                     TLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLRGSQRQPLMPVVFTSMLGMTLEGM
                     TIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGELMFSWYCMDNVLEPGAAEAMFND
                     YCAILQAVIAAPESLKTLASGIARHIPRRRWPLNAQADYDLRDIEQATLEYPGIRQAR
                     AEITEQGALTLDIVMADDPSPSAAMPDEHELTQLALPLPEQAQLDELEATWRWLEARA
                     LQGIAATLNRHGLFTTPEIAHRFSAIVQALSSQASHQRLLRQWLQCLTEREWLIREGE
                     SWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCIARHDALFSGQCSPLELLFNEQHR
                     VTDALYRDNPASACLNRYTAQIAALCSAERILEVGAGTAATTAPVLKATRNTRQSYHF
                     TDVSAQFLNDARARFHDESQVSYALFDINQPLDFTAHPEAGYDLIVAVNVLHDASHVV
                     QTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGFIEGLSGYRDFRRRDEKPMLTRSA
                     WQEVLVQAGFANELAWPAQESSPLRQHLLVARSPGVNRPDKKAVSRYLQQRFGTGLPI
                     LQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPALEKQVAELWQSLLSRPVARHHDF
                     FELGGDSLMATRMVAQLNRRGIARANLQDLFSHSTLSDFCAHLQAATSGEDNPIPLCQ
                     GDGEETLFVFHASDGDISAWLPLASALNRRVFGLQAKSPQRLATLDQMIDEYVGCIRR
                     QQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRMVLIDPVCRQDFCCENRAALLRLL
                     AEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMVSQNLTLQAAETWLDNIAHLLRLL
                     TEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGWINNADDAVIEASHWQIMMEAPHV
                     QACAQHITRWLCATSTQPENTL"
     gene            4374..5474
                     /gene="ybtU"
                     /locus_tag="ACG0BW_004014"
     CDS             4374..5474
                     /gene="ybtU"
                     /locus_tag="ACG0BW_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFK7260915.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAVAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            5471..6274
                     /gene="ybtT"
                     /locus_tag="ACG0BW_004015"
     CDS             5471..6274
                     /gene="ybtT"
                     /locus_tag="ACG0BW_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFK7260916.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            6278..7855
                     /gene="ybtE"
                     /locus_tag="ACG0BW_004016"
     CDS             6278..7855
                     /gene="ybtE"
                     /locus_tag="ACG0BW_004016"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFK7260917.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            7986..10007
                     /gene="fyuA"
                     /locus_tag="ACG0BW_004017"
     CDS             7986..10007
                     /gene="fyuA"
                     /locus_tag="ACG0BW_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFK7260918.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         2140 a         3011 c         2905 g         2093 t
ORIGIN      
        1 gacgggctgg cccagcagtt ttccaccctt gatgcgccga aaacgctctc tgtcgcctgg
       61 ggcgcatggg gagaaagcgg tcgggcggcc acgccggaaa tgctggcgac gctcgccagc
      121 cgaggtatgg gcgcgttaag cgatgccgaa ggctgctggc acctggaaca ggcggtgatg
      181 cgcggcgccc cgtggcgact ggcgatgcgc gtttttaccg acaaaatgcc cccgttacaa
      241 caggctctgt ttaacatcag cgccacagaa aaagccgcaa cgccggtcat tcctcctgct
      301 gatgacaacg cctttaacgg cagcctgagc gatgaaacag cggtgatggc atggctgaaa
      361 aagcggattg cggttcagct aaggctgagc gatccggcgt cactgcatcc aaaccaggat
      421 ctgttgcaac tcggcatgga ctcgctgctc ttccttgaac tcagtagcga tattcagcac
      481 tacctgggtg tacgcatcaa tgcggaacgg gcgtggcagg atctgtctcc tcatggactc
      541 acgcagctta tctgttctaa gccagaggcg acgcctgccg cttcgcagcc ggaagtgttg
      601 cggcacgacg ccgacgagcg ttatgcgccc ttccctttga cgcccattca gcacgcctac
      661 tggctggggc gaacccacct cattggctat ggcggcgtcg cctgtcacgt cctgtttgag
      721 tgggataaac gccacgatga gttcgatctc gccatactgg agaaagcatg gaaccagctc
      781 atcgcacgcc acgatatgtt gcgtatggtg gttgatgccg acgggcagca gcgaatcctg
      841 gcgacaacgc cggagtatca catcccgcgt gacgatctgc gcgcgctttc cccggaagaa
      901 cagcgcatcg cgctggaaaa acggcggcat gaactgagct atcgcgtttt gcctgccgac
      961 cagtggcctc tttttgagct ggtggtcagc gaaatcgacg attgccatta ccgtctgcat
     1021 atgaacctcg accttttgca gtttgatgtg cagagtttta aagtcatgat ggacgacctg
     1081 gcgcaggtct ggcgcggtga aacgctggca ccgctcgcta ttaccttccg tgattatgtg
     1141 atggctgaac aggcgcgccg acagacatcg gcatggcacg atgcctggga ttactggcag
     1201 gaaaaactgc cgcaactgcc cttagcgcca gagctgccgg tggttgagac gcccccggaa
     1261 acgccacact tcaccacctt caaatcgacg atcggcaaga cagaatggca ggccgtgaaa
     1321 cagcgctggc agcagcaagg cgtcacaccg tctgccgcgc tgctcacgct gtttgccgcc
     1381 acccttgagc gctggagccg taccacaaca tttacgctga acctgacgtt cttcaatcgc
     1441 cagccgatcc atccgcaaat caaccagttg attggtgatt ttacctccgt cacgctggtt
     1501 gattttaact tctcagcgcc ggtgacgttg caagagcaga tgcaacagac ccaacagcgc
     1561 ctctggcaaa acatggcgca cagtgaaatg aacggtgttg aggtgatccg tgagctgggc
     1621 cgcctgcgcg gatcacaacg tcaaccgctg atgccggtag tgtttaccag tatgctgggg
     1681 atgacgctgg aaggcatgac tatcgatcag gcgatgagcc atctgttcgg cgaaccctgc
     1741 tatgtattca cgcaaacgcc gcaggtctgg ctggatcatc aggtcatgga gagcgacggc
     1801 gagttgatgt ttagctggta ctgcatggac aacgtgctgg aacccggcgc tgccgaggcg
     1861 atgtttaatg actattgcgc catcctgcaa gccgtcatcg ccgcccctga aagcctgaag
     1921 actctcgcca gcggcatcgc caggcacatt ccccgccgac gctggccgct gaacgcgcag
     1981 gcggactacg acctgcggga tattgagcag gcgacgctcg aataccccgg catccggcag
     2041 gccagagcgg aaataaccga acagggcgcg ttgacgctgg atatcgtgat ggccgacgat
     2101 ccttcgccat cagcggcgat gcctgatgag cacgaactta cccaactggc gctgccgttg
     2161 cctgagcagg cgcagcttga tgagctggag gcgacctggc gctggctgga ggcgcgtgcg
     2221 ctacagggga tcgcggctac gctaaatcgt cacggcctgt ttaccacgcc ggagatcgcc
     2281 catcgcttta gcgcaatagt acaggcgctg tcctcgcaag catctcacca gcgtctgctg
     2341 cgccagtggc tacagtgtct gacggaaaga gagtggttaa tccgcgaagg tgaaagctgg
     2401 cgctgccgca ttccgctcag cgagattcct gagcctcagg aagcgtgccc gcaaagccaa
     2461 tggagccagg cgctggcgca gtatctggaa acctgcatcg cccggcacga cgccctcttc
     2521 tccgggcagt gttctccgct ggaattgctg ttcaacgagc agcatcgcgt taccgacgcg
     2581 ctgtatcgcg acaaccccgc cagcgcctgt ctgaatcgct ataccgcgca gattgccgcc
     2641 ttgtgcagcg cagaacggat tctggaggtt ggcgccggaa ccgcagccac taccgcgccg
     2701 gtgctgaagg ccacgcggaa cacgcgacag tcgtaccact tcacggacgt ctccgcgcag
     2761 ttcctcaatg acgccagagc ccgtttccat gatgaatcgc aggtgtctta tgccttgttc
     2821 gacatcaacc agccgctgga tttcaccgcc cacccggagg cgggttacga cctgatcgtt
     2881 gccgtcaatg tgctccacga cgccagccat gtcgtccaga cgttgcgcag attaaaactg
     2941 ttgctgaaag ccggcggacg tttgctgatc gttgaagcga cggagcgaaa cagcgtattc
     3001 cagctggcga gcgtgggctt tattgaggga ttaagcggat accgcgattt ccgccgccgg
     3061 gatgagaaac cgatgctcac ccgctccgca tggcaggagg ttctagttca ggccgggttt
     3121 gcaaacgagc tggcgtggcc cgcgcaggaa tcgtcgccgc tgcgccagca tctgctggta
     3181 gcgcgttcgc ctggcgtaaa tcgcccggat aaaaaagccg tgagccgcta tttacagcag
     3241 cgctttggca ccggtctgcc cattttacag atccggcaaa gagaagcgtt atttacgccg
     3301 ctgcatgccc cgtctgatgc gccgactgag ccagccaaac ccacgccagt tgccgggggg
     3361 aatccggcac tggaaaaaca ggtggctgaa ctctggcaat cgctgctgtc tcgccccgtg
     3421 gcaaggcatc acgacttttt cgaactgggc ggcgacagcc tgatggcgac aaggatggtc
     3481 gcgcagctaa accggagagg gattgccagg gctaaccttc aggatctgtt cagccattcg
     3541 acgctgagcg acttctgcgc ccatctacag gcggctacgt caggagagga caacccgata
     3601 cccctttgcc agggcgacgg tgaggaaacc ctgtttgtct tccacgcttc agacggcgat
     3661 atcagcgcct ggctgccgct cgctagcgcg ttgaacaggc gcgttttcgg cctgcaagca
     3721 aaatcgccgc agcgccttgc cacgctcgac cagatgatcg atgagtatgt cgggtgcatc
     3781 cgtcgtcagc agcctcacgg cccttatgtg ctggcgggtt ggtcgtatgg cgcgtttctt
     3841 gcggcgggcg ccgcacaacg cctgtacgcc aaaggcgagc aggttcggat ggtgttaatc
     3901 gatcccgtgt gccgacagga tttctgttgc gaaaaccggg cggccctgct gcgcctgtta
     3961 gccgaaggac aaacgcctct ggcactgccc gaacatttcg accagcagac gcccgacagc
     4021 cagcttgccg actttatcag cctcgctaaa acggccggta tggtgtcgca aaacctgacg
     4081 ctgcaagcgg cagaaacgtg gctcgacaac atcgcgcatc tgctgcgttt actgactgag
     4141 catacgccgg gcgaaagcgt tccggtcccc tgtctcatgg tgtatgccgc cgggagaccc
     4201 gcgcactgga cgccagcaga aaccgagtgg cagggctgga taaacaacgc cgacgacgct
     4261 gtgattgaag ccagccactg gcaaatcatg atggaagccc cccacgttca ggcttgtgcg
     4321 caacacatta cgcgctggct ttgcgcaacc tcaacgcaac cggagaacac gttatgatgc
     4381 cgtccgcctc cccaaaacaa cgcgtactga ttgtgggcgc caaatttggc gaaatgtacc
     4441 tgaatgcctt tatgcagccc ccggaggggc tggaactggt cggcctgctg gcgcagggaa
     4501 gcgcccgttc aagagagctg gctcatgcgt ttggcattcc gctgtatacc tcgccggaac
     4561 agataaccag gatgccggat atcgcctgta ttgtcgtgcg ttcgaccgtc gccggaggaa
     4621 ccggtacgca gcttgccaga cactttctga cgcgcggcgt acacgtcatt caggagcatc
     4681 ccctccaccc ggatgacatc agttccttac aaacgctggc gcaggaacag ggctgctgct
     4741 attgggtaaa cacgttttat ccgcatacgc gcgcgggacg cacatggctt cgcgatgcgc
     4801 agcagctgcg tcgctgtctg gctaagacgc cgcaggtggt ccacgccacc accagccgac
     4861 agttgctcta ctccactctg gatttgttgt tgctggctct gggcgttgat gccgtcgccg
     4921 tggagtgcga cgtggttggc agctttagcg attttcactg tctgagactc ttctggccgg
     4981 agggagaagc ctgcctgctg cttcagcgct atctcgatcc tgacgatcca gatatgcata
     5041 gccttatcat gcaccgcctg ctgctgggct ggccggaagg tcatctttcg ctggaggcga
     5101 gctacggccc cgtcatctgg tcatccagcc tgtttgtcgc agaccatcag gagaacgccc
     5161 acagcctcta ccgcagaccg gagatcctgc gcgatctgcc gggtctgacg cgcagcgcgg
     5221 cgcccctgag ctggcgcgac tgctgcgaaa ccgtcgggcc ggagggcgtc agctggttgc
     5281 tacaccagtt gcgcagccat ctggcgggcg aacatccacc agcggcctgc caaagcgtac
     5341 atcagatcgc gttatcacgt ttatggcagc agattttacg taagaccggc aatgcggaga
     5401 tcagacgcct gacgccgccg caccacgacc ggttagccgg tttctacaac gatgatgata
     5461 aggaggcgct gtgacgcaat ctgcaatgtg cataccgctg tggcccgccc ggaacggcaa
     5521 tactgcgcat ctggtcatgt gccctttcgc tggcggcagc agtagcgcgt ttcgccactg
     5581 gcaagctgag caactggctg attgcgcgct ttctctggtg acctggccgg ggcgcgatcg
     5641 ccttcgccat ctggaaccgc tcagaagcat tacacaactg gcggcactgc tggcgaacga
     5701 gctggaagca tccgtatcgc ctgacacgcc gcttttactc gccgggcaca gcatgggggc
     5761 gcaggtggcg tttgaaacct gccgacttct ggagcaacgg gggcttgcgc cacaaggact
     5821 gattatttcc gggtgccatg ccccgcatct gcattctgaa cgccagctca gccatcgcga
     5881 tgatgccgac tttatcgctg agctgataga cattggcgga tgttctcctg aactgcggga
     5941 aaaccaggaa ttaatgtcgc tgtttcttcc tcttctgcgc gctgattttt acgccaccga
     6001 gagctatcac tacgactcgc ccgacgtctg tccgccgctg cgcacgcctg cgctgttatt
     6061 gtgcggcagc cacgatcgcg aagcctcctg gcagcaggtc gatgcctggc gtcagtggct
     6121 gagccacgtt acaggcccgg tggtgattga cggcgatcat ttctatccca ttcaacaagc
     6181 ccggtccttt tttacgcaga ttgtccgcca ttttccccac gcattttctg caatgaccgc
     6241 gttgcaaaaa cagcccagta cttcagaaag gtgacgcatg aattcttcct ttgaatctct
     6301 gattgaacag tatcccttac ccattgccga acagttgcgc cactgggcgg cccgttatgc
     6361 ctcgcgaatt gccgtcgttg atgcaaaggg gtcgttaacc tacagcgcgc ttgatgcaca
     6421 agttgacgaa cttgccgcag gtctgtcatc actgggtttg cgttcggggg agcatgtaat
     6481 tgtgcagctt cccaacgaca acgcgtttgt taccctgctg ttcgccttgt taagactggg
     6541 cgttatcccc gtgctggcga tgccctcgca acgggcgctg gatatcgacg cgctgattga
     6601 gctggcgcaa cccgtcgctt acgttattca cggggaaaac cacgcagagc tggcccgaca
     6661 gatggcgcac aaacacgcct gcttgcgtca tgttctggtc gctggagaga ccgtgagcga
     6721 cgattttacg ccgctcttct cccttcacgg tgagcgacag gcatggccgc agcctgatgt
     6781 ttccgccacc gcgttgttgt tgctctcagg cggcacaacc ggcacgccaa aactcatccc
     6841 gcgccgacat gccgactata gctataactt cagcgcttct gctgaactgt gcggcatcag
     6901 ccaacagagc gtgtatctcg ccgtcctccc ggtggcgcat aactttccgc tggcctgccc
     6961 cggtattctg ggaacgcttg cctgcggcgg aaaagtggtg ctgaccgaca gcgccagctg
     7021 tgatgaggtg atgcctttaa tcgcgcagga aagagtgact cacgtcgccc tggttccggc
     7081 gctggcgcaa ttatgggtgc aggccaggga gtgggaagac agcgaccttt cgtcgctgcg
     7141 cgtcattcag gcaggcggcg cccggctcga cccgacgctt gctgagcagg ttatcgccac
     7201 ctttgactgt accctgcaac aggttttcgg tatggcggaa ggcctgctct gttttacccg
     7261 gctggacgat ccgcatgcca ccattctcca cagccagggg cgcccgttgt cccctctgga
     7321 tgaaatccgc atcgttgatc aagacgagaa cgacgtcgcg ccgggcgaaa ccgggcaatt
     7381 gttaacgcgc ggcccttata ccatttcggg ctattaccgc gcccctgccc acaacgcgca
     7441 ggcctttacc gcgcaagggt tttaccgcac aggcgacaat gtcaggctgg atgaggtggg
     7501 gaacctgcac gttgagggac gcataaaaga gcagatcaac cgcgccggag aaaaaatagc
     7561 cgcggctgaa gtggaatcgg cactgctgcg tttagcggaa gtgcaagatt gcgcggtggt
     7621 cgccgcgccg gacacgctgc ttggcgagcg gatttgcgcg tttatcatcg cgcagcaggt
     7681 gccaactgac tatcagcagt tgcgtcaaca actgacccgt atggggctca gcgcgtggaa
     7741 aattcctgac caaatcgagt ttctggacca ctggccgctc accgccgtcg gcaagataga
     7801 caaaaaacgc ctgacggctc tcgccgtcga ccgttatcgc cattctgccc aataagcgca
     7861 aaccgacccg aaacaggttg aaataaaccc gtttcgggta gcactactat tagaaatagt
     7921 tatcattttc aattcaccat tgtcggtatt tttggcgttt cgccgtctta cagggactca
     7981 caacaatgaa aatgacacgg ctttatcctc tggccttggg gggattattg ctccccgcca
     8041 ttgctaatgc ccagacttca cagcaagacg aaagcacgct ggtggttacc gccagtaaac
     8101 aatcttcccg ctcggcatca gccaacaacg tctcgtctac tgttgtcagc gcgccggaat
     8161 taagcgacgc cggcgtcacc gccagcgaca aactccccag agtcttgccc gggctcaata
     8221 ttgaaaatag cggcaacatg cttttttcga cgatctcgct acgcggcgtc tcttcagcgc
     8281 aggacttcta taaccccgcc gtcaccctgt atgtcgatgg cgtccctcag ctttccacca
     8341 acaccatcca ggcgcttacc gatgtgcaaa gcgtggagtt gctgcgaggc ccacagggaa
     8401 cgttatatgg caaaagcgct cagggcggga tcatcaacat cgtcacccag cagccggaca
     8461 gcacgccgcg cggctatatt gaaggcggcg tcagtagccg cgacagttat cgaagtaagt
     8521 tcaacctgag cggccccatt caggatggcc tgctgtacgg cagcgtcacc ctgttacgcc
     8581 aggttgatga cggcgacatg attaaccccg cgacgggaag cgatgactta ggcggcaccc
     8641 gcgccagcat agggaatgtg aaactgcgtc tggcgccgga cgatcagccc tgggaaatgg
     8701 gctttgccgc ctcacgcgaa tgtacccgcg ccacccagga cgcctatgtg ggatggaatg
     8761 atattaaggg ccgtaagctg tcgatcagcg atggttcacc agacccgtac atgcggcgct
     8821 gcactgacag ccagaccctg agtgggaaat acaccaccga tgactgggtt ttcaacctga
     8881 tcagcgcctg gcagcagcag cattattcgc gcaccttccc ttccggttcg ttaatcgtca
     8941 atatgcctca gcgctggaat caggatgtgc aggagctgcg cgccgcaacc ctgggcgatg
     9001 cgcgtaccgt tgatatggtg tttgggctgt accggcagaa cacccgcgag aagttaaatt
     9061 cagcctacga catgccgaca atgccttatt taagcagtac cggctatacc accgctgaaa
     9121 cgctggccgc atacagtgac ctgacctggc atttaaccga tcgttttgat atcggcggcg
     9181 gcgtgcgctt ctcgcatgat aaatccagta cacaatatca cggcagcatg ctcggcaacc
     9241 cgtttggcga ccagggtaag agcaatgacg atcaggtgct cgggcagcta tccgcaggct
     9301 atatgctgac cgatgactgg agagtgtata cccgtgtagc ccagggatat aaaccttccg
     9361 ggtacaacat cgtgcctact gcgggtcttg atgccaaacc gttcgtcgcc gagaaatcca
     9421 tcaactatga acttggcacc cgctacgaaa ccgctgacgt cacgctgcaa gccgcgacgt
     9481 tttataccca caccaaagac atgcagcttt actctggccc ggtcgggatg cagacattaa
     9541 gcaatgcggg taaagccgac gccaccggcg ttgagcttga agcgaagtgg cggtttgcgc
     9601 caggctggtc atgggatatc aatggcaacg tgatccgttc cgaattcacc aatgacagtg
     9661 agttgtatca cggtaaccgg gtgccgttcg taccacgtta tggcgcggga agcagcgtga
     9721 acggcgtgat tgatacgcgc tatggcgcac tgatgccccg actggcggtt aatctggtcg
     9781 ggccgcatta tttcgatggc gacaaccagt tgcggcaagg cacctatgcc accctggaca
     9841 gcagcctggg ctggcaggcg actgaacgga tgaacatttc cgtctatgtc gataacctgt
     9901 tcgaccgtcg ttaccgtacc tatggctaca tgaacggcag cagcgccgtc gcgcaggtca
     9961 atatgggtcg caccgtcggt atcaatacgc gaattgattt cttctgatta ttgtaaaagg
    10021 gataccgaaa aggtatccct tttacaccac tagttaaaac cagtaactca gcagagtcgc
    10081 aaaaaatatt aatccatagt gattatttaa acaatgaaat tgcgattagg ataaatagat
    10141 ttaactttc
//
LOCUS       DAYPHN010000168        10010 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000168 DAYPHN010000000
VERSION     DAYPHN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.168"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..124
                     /locus_tag="ACG0BW_004018"
     CDS             <1..124
                     /locus_tag="ACG0BW_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260919.1"
                     /translation="MTLDEINNTYCNNDYYNEAIREEPIDFLDRSFSSSSWPFQ"
     gene            436..1824
                     /gene="narU"
                     /locus_tag="ACG0BW_004019"
     CDS             436..1824
                     /gene="narU"
                     /locus_tag="ACG0BW_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFK7260920.1"
                     /translation="MALQNEKTSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFIPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1919..5659
                     /gene="narZ"
                     /locus_tag="ACG0BW_004020"
     CDS             1919..5659
                     /gene="narZ"
                     /locus_tag="ACG0BW_004020"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFK7260921.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVNGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKNNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALNSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQACVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5656..7200
                     /gene="narH"
                     /locus_tag="ACG0BW_004021"
     CDS             5656..7200
                     /gene="narH"
                     /locus_tag="ACG0BW_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFK7260922.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7200..7895
                     /gene="narW"
                     /locus_tag="ACG0BW_004022"
     CDS             7200..7895
                     /gene="narW"
                     /locus_tag="ACG0BW_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFK7260923.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDTLLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7892..8572
                     /gene="narI"
                     /locus_tag="ACG0BW_004023"
     CDS             7892..8572
                     /gene="narI"
                     /locus_tag="ACG0BW_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFK7260924.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8651..9544
                     /gene="yddE"
                     /locus_tag="ACG0BW_004024"
     CDS             8651..9544
                     /gene="yddE"
                     /locus_tag="ACG0BW_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFK7260925.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLSGK
                     HRVTIENHNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILSGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLHVKGHQGRALGRDGVIEVTVTIRNNQP
                     EKVTISGTAVILFHAEWAIEF"
     gene            complement(9633..>10010)
                     /locus_tag="ACG0BW_004025"
     CDS             complement(9633..>10010)
                     /locus_tag="ACG0BW_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7260926.1"
                     /translation="ACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSA
                     LADKGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQ
                     KRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT         2335 a         2591 c         2630 g         2454 t
ORIGIN      
        1 tatgacatta gatgaaataa acaatactta ctgtaataac gattattaca atgaggcgat
       61 aagagaggaa ccgatagact ttctggacag atcgttttcc tccagctcat ggccttttca
      121 gtgaataaat catcactaat tctataagtg tctggtcttg ttgaaaatat aaaccctttg
      181 gattcatcat ctgaagggtt ggatcatgac atgcatacat agcttaacat ccgtttaatg
      241 gttagcttgt tagttacatt tagtaacaca tatctgtctg tttaatcccc attctgcccc
      301 cgcggttacg ctttcttatc tattctttca atcagcactt cacctaattc atccttaatt
      361 ctaataactg gctattgcgc cttacctcct gttttgtctt tttttcatta ccaatgtgta
      421 catgtgagga acaatatagc actgcaaaat gagaaaacta gtcgttatct tttgcgcgac
      481 tggaaaccag aaaatccggc cttctgggaa aataaaggaa aacatattgc acgaagaaac
      541 ctctggatat cagtcagttg tctacttctt gccttctgtg tctggatgct atttagcgca
      601 gttactgtta atctcaataa aatcggtttt aatttcacta ccgatcaact ctttttatta
      661 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      721 atatttggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      781 tggctcggaa ttgccgtgca gaatccgaat actccttttg ggatatttat cgttatcgct
      841 ttgctatgcg gttttgcagg tgcgaacttt gcttcgagca tgggcaatat cagtttcttc
      901 ttcccaaaag ccaaacaagg gagcgcactt gggattaatg gcggattagg aaacttaggt
      961 gtaagtgtga tgcagctggt tgcaccgctg gtcattttta tacccgtatt tgcctttctc
     1021 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
     1081 atttgggtgc cattactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
     1141 gccagttcac gcgcgtcaat tgccgaccag ctgccagtgt tacaacgcct gcatctctgg
     1201 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
     1261 gccatgctgg cgaaaactca gttccctgat gtgaatattc tgcgcctggc gttctttggc
     1321 ccatttattg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
     1381 gtgcgggtga cgttgatcaa cttcattttt atggcgattt tcagcgccct gctgttcctt
     1441 accttaccgg gcacaggctc cggtaatttc atcgcatttt acgccgtatt tatggggttg
     1501 tttctgaccg caggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
     1561 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagagagaa
     1621 gccgtcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1681 tttttcattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtaggcgcg
     1741 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1801 cggcggaagt tcagccaaaa ataagagatc gatgccagac acgtcttatc aggctacaac
     1861 cgtgtcgcat ccgacaaggc atcagatccc aattcattcc tggagcagga gttatgtcat
     1921 gagtaaactt ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg
     1981 tcacggacag gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca
     2041 gttcgacaaa atcgtgcgtt ccacccacgg tgttaactgt acaggctcct gtagctggaa
     2101 aatctacgtt aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac
     2161 tcgccctgac ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg
     2221 gtatctctac agcgctaacc gtctgaaata cccgctcatt cgtaaacgac tgattgaact
     2281 gtggcgcgaa gccctcaagc aacacagcga tccggtactg gcgtgggcat cgattatgaa
     2341 cgatccgcaa aagtgcctga gctacaaaca agtacgtgga cgcggcggct ttatccgctc
     2401 taactggcag gagctaaatc agctgattgc tgccgctaac gtctggacca tcaaaactta
     2461 cggcccggat cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc
     2521 cgccggaaca cgttatctgt cgctgttagg tggcacctgt ttaagtttct atgactggta
     2581 ttgcgacctg ccgcctgcct cgccgatgac ctggggcgag caaaccgacg tgccagaatc
     2641 tgccgactgg tataactcca gctacatcat cgcctggggg tctaacgtac ctcagacacg
     2701 tacgccggac gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat
     2761 tacccctgac tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg
     2821 cactgatagc gccctggcga tggcaatggg ccatgtcatt ttaaaagagt ttcatctcga
     2881 taatcccagc gattatttta tcaactactg ccgccgctac agcgacatgc cgatgctggt
     2941 aatgctggaa cctcgcgacg acggcagcta cgttcccggg cgcatggtcc gcgcatctga
     3001 cctggtgaat ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtgg cggttaatac
     3061 cgcaggtgaa ctggtggtgc cgaacggttc gattggtttt cgctggggag aaaaaggcaa
     3121 atggaatctt gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ctctgctcgg
     3181 tcaacatgat gccgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca
     3241 ttttcgcagc gtaaaaaaca atcctgtcct ggtgcgtcaa ttacccgtta aaaacctgac
     3301 attagccgat ggcagcacct gtccggtggt cagcgtttat gatttggtac tggcgaatta
     3361 cggcctcgat cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc
     3421 gtacacccca gcctggggtg agcaaattac cggtgtgccg cgccagtata ttgaaaccat
     3481 cgctcgtgaa tttgccgata ctgcccataa aacgcatgga cgctcgatga ttatcctcgg
     3541 cgcaggtgtt aaccactggt accacatgga catgaactac cggggaatga tcaacatgct
     3601 gatcttctgc ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggtcagga
     3661 aaaactgcgc ccgcaaaccg gctggttgcc gctggccttt gcgctcgatt ggaaccgacc
     3721 accgcgccag atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga
     3781 aaaagtctct gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca
     3841 tctgattgat ttcaacgttc gtgccgaacg tatgggctgg ctaccttctg cgccgcagct
     3901 ggggcgtaac ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga
     3961 atttaccgcc caggcgctga attcgggcga tttacgcatg gcctgcgaac aaccagatag
     4021 cggcagcaat catccgcgta atttgtttgt ctggcgttct aacctgcttg gctcctccgg
     4081 caaaggccac gagtatatgc agaagtatct gctgggaacc gaaagtggga ttcagggtca
     4141 ggaactcggt gccagcgacg ggatcaaacc agaagaagtc gagtggcaaa ctgcggcgat
     4201 tgaaggcaag ctcgacctgc tggtgacgct cgacttccgt atgtccagta cctgcctgtt
     4261 ctccgatatc gttctgccca ccgccacctg gtacgaaaaa gacgatatga acacctcgga
     4321 tatgcatccg tttattcatc ccctatctgc ggcggtcgat ccggcgtggg aatcacgcag
     4381 cgactgggaa atctacaaag gtattgccaa agccttttcg caagcgtgcg tgggccatct
     4441 tggcaaagaa accgacgtgg tattacaacc actgctgcat gactctccgg cagaactctc
     4501 acagccgtgt gaagtgctcg actggcgcaa aggcgaatgc gatctgattc caggtaaaac
     4561 tgcgcccaat attgtggcgg tggaacgcga ctaccctgct acctatgaac gctttacctc
     4621 gctcgggcca ttgatggaca aacttggcaa cggcggtaaa gggatttcgt ggaataccca
     4681 ggatgaaatc gatttcctcg gcaaactcaa ctacaccaag cgtgaaggcc cagcacaggg
     4741 gcgtccgctg attgacaccg ccattgacgc atcagaagtg attctggcgt tggcaccgga
     4801 aaccaacggt catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccggacgcga
     4861 acatacccat ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc
     4921 gcagccacgt aaaattatct ccagccccac atggtctggt ctggaaagcg atcacgtctc
     4981 ctataacgcg ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg
     5041 ccagcagctt tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta
     5101 tcgcccgcct atcgacaccc gtagcgtcag tgaaatgcgc cagataccgc caaacggctt
     5161 cccggaaaaa gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta
     5221 cagtgaaaac ctgctaatgc tgacgctctc tcgcggtgga ccaattgtct ggatcagcga
     5281 aaccgatgcc cgtgaactga ccattgtcga taacgactgg gtggaagtgt ttaacgccaa
     5341 cggcgcgctg actgcccgcg cggtggtcag ccaacgtgta ccgccgggta tgaccatgat
     5401 gtatcacgcc caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg
     5461 cggcattcat aactcggtca cccgcgtttg tccgaaacca acgcatatga ttggcggtta
     5521 cgcgcagctg gcctggggct ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt
     5581 catcatgatc cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt
     5641 acaggaggcg aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa
     5701 tgtatcggct gccatacctg ttcggtgacc tgtaaaaacg tctggaccgg acgcgaaggc
     5761 atggagtacg catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac
     5821 tgggaagatc aggaagagtg gcaaggcggc tgggtgcgtg atgtgaatgg caagatccgc
     5881 ccgcgtctgg gcagcaagat gggcgtaata accaaaatct tcgctaaccc ggtggtgccg
     5941 cagattgatg attactacga acctttcact ttcgactacg aacatttgca tagcgcaccg
     6001 gaaggcaaac atatccctac tgctcgtccg cgttcactga ttgacggcaa acggatggac
     6061 aaagtgatct gggggccaaa ctgggaagaa ttgttgggcg gcgaattcga aaaacgcgcc
     6121 cgtgaccgca acttcgaggc catgcaaaag gagatgtacg ggcagtttga aaacaccttc
     6181 atgatgtacc tgccgcgctt gtgcgaacac tgcctcaatc ccagttgcgt ggcgacctgc
     6241 ccaagcggcg ctatctacaa acgcgaagaa gacggcattg tgctgatcga tcaggataaa
     6301 tgccgtggct ggcgtttgtg cattagcggt tgtccgtaca aaaaaatcta cttcaactgg
     6361 aaaagcggca agtcagaaaa atgtatcttc tgttacccac gaattgagtc aggccaaccc
     6421 accgtgtgct cagaaacctg cgtgggtcgc atccggtatc tgggcgtgct gctttacgac
     6481 gctgaccgca tcgaggaagc ggcgagcacc gagcgtgaag ttgacctcta tgaacgccag
     6541 tgcgaagtat tcctcgatcc acacgatccc tcagtgatcg aggaagccct gaaacaaggt
     6601 attccacaaa acgtgattga agctgcccag cgttcgccag tctacaaaat ggcgatggac
     6661 tggaaactgg cgctaccgct gcaccctgaa taccgcaccc tgccgatggt ctggtacgtt
     6721 cctccgctgt caccgattca gtcctacgca gatgcgggcg gtttgccgaa aagcgaaggc
     6781 gtgctgccag ccatcgagag cctgcgtatt cctgtgcaat atctcgccaa tatgttaagt
     6841 gccggcgata ccggtccggt actgcgggcg ctaaaacgga tgatggcgat gcgccactat
     6901 atgcgttcac aaaccgtgga aggcgttacc gatacccgcg ccattgacga agtaggcctg
     6961 agcgtcgccc aggtcgaaga gatgtatcgc tacctcgcca ttgccaacta tgaagaccgt
     7021 tttgtcattc caaccagtca tcgggaaatg gcgggcgatg ccttcgcaga acgcaatggc
     7081 tgcggtttta cctttggcga cggttgccac ggctcggaca gcaaattcaa cctgttcaac
     7141 agtagccgta tcgatgccat caacatcacc gaagtacgcg acaaagcgga gggcgaataa
     7201 tgcagatcct caaagtgatc ggcctgttga tggagtatcc ggacgagctg ttatgggaat
     7261 gtaaggagga cgcgctggcg ttgatccgcc gcgacgcacc gatgcttacg gattttaccc
     7321 gcaacctgct taacgcgccg ctgctggata aacaggccga atggtgcgaa gtgtttgacc
     7381 gtggacgtac cacatcgctg ctgctgttcg aacatgttca tgccgagtcc cgcgatcgcg
     7441 gccaggcaat ggtggacctg ctggcggagt atgaaaaggt cggcctgcag ttggattgtc
     7501 gagaactgcc cgattatcta ccgctgtatc tggagtactt aagcgtactg ccggacgatc
     7561 aggcgaaaga aggcttgctc aacgttgcgc cgatcctcgc cctgcttggc ggacgcttaa
     7621 aacaacgcga ggcaccgtgg tacgcgttat ttgacaccct gttgcaactg gcaggaagta
     7681 cactttcaag tgacagtgtc acaaaacagg tgaacagtga ggagcgcgat gacacccgcc
     7741 aggcactgga tgcggtgtgg gaagaggaac aggtgaagtt tattgaagat aacgccacgg
     7801 cgtgtgacag ctcgccgctt aatcaatatc agcgacgctt tagccaggat gtcgcgccgc
     7861 agtatgtcga catcagtgcg ggaggtggga aatgattcag tatctgaacg tcttttttta
     7921 tgatatctac ccgtacattt gtgcgacagt gtttttcctc ggcagctggc tgcgctacga
     7981 ctacgggcag tatacctggc gcgcctcctc gagtcagatg ctcgataaac gcgggatggt
     8041 gatatggtcg aatctgttcc atatcggcat tttggggatt ttcttcgggc acctgttcgg
     8101 catgttaacg ccgcactgga tgtacgcgtg gtttctgccg gtggcggtaa aacagcagat
     8161 ggcgatggtg ctcggtggta tttgcggtgt attgacgttg attggcggcg caggactgct
     8221 gtggcgcagg ctgactaatc agcgcgtgcg tgccacttcc actacgccgg atattatcat
     8281 catgagcatt ttgctgattc agtgtctgct ggggctaagt accataccgt tttcagcaca
     8341 gtatcctgat ggtagcgaaa tgatgaagct ggtgggttgg gcgcaaagca ttgtcacttt
     8401 ccgtggtgga tcgtcagaaa tgcttaacgg cgtagcgttc gttttccgcc tgcatctggt
     8461 gttgggaatg accatcttcc tgctcttccc gttcacccga ctggtgcatg tgtggagcgc
     8521 gccgtttgag tactttactc gtcggtatca gattgtgcgc tcgcggcgat aatttcaccc
     8581 tggccggata gcccttcgct ctccggccac ttgcaggcta tgatagttct ccagccacag
     8641 gagaacgtta atgaaaccgc aggtgtacca cgtcgatgct tttacctcac aaccgtttcg
     8701 cggcaattct gccggtgttg ttttccccgc cgataatctc agtgaagcgc aaatgcagct
     8761 tatcgcccgc gaattaggtc attcggaaac tgcttttctg ctgcacagcg acgacagcga
     8821 tgtgcgcatc cgctacttta cgccaacagt tgaagtgccg atttgtggtc acgcgacagt
     8881 agctgcgcac tatgtacgtg cgaaggtgtt aggtttagga aattgcacgg tctggcaaac
     8941 gtcactgtca ggaaaacatc gcgtgactat cgaaaatcac aacgatgatt atcgtatttc
     9001 gctggaacaa ggtacgccgg gctttgagcc accgctggaa ggtgaaacac gtgcagcgat
     9061 tatcaacgcg cttcatctca ctgaggacga tattttgtca ggcttgccaa ttcaggtggc
     9121 aaccacagga cactcaaaag tgatgatccc actgaaacca gaagtggata tcgacgccct
     9181 ttcgcctgat cttgctgcgc tgaccgctat cagcaaacag attggctgca atggtttctt
     9241 cccgttccag atccgcccag gcaaaaatga aaccgatggt cgcatgttct cgcctgcgat
     9301 tggtattgtg gaagatccgg tgactggcaa tgccaatggc ccgatgggtg catggttggt
     9361 acatcacaac gtattgcccc acgatggcaa cgtgttgcat gttaaaggcc atcaggggcg
     9421 cgcattgggg cgtgacggcg tgattgaagt gacggtgaca attcgcaata accaaccgga
     9481 gaaagtcacc atttctggca cagcggtgat tttgtttcat gccgaatggg caattgaatt
     9541 ctgaaaccgt tgcctgatgc gcttcgctta tcaggtctat gtatgccgga taaggcgttt
     9601 tgaactgaac cccaaaacct ggacagtttc agttaaccgg ctttttgata ctcagtccgg
     9661 tattctaccg ggctgagtcc cccaagactg agttttattc ttttttgatt ccagtaagtt
     9721 atgtattcgt taacggcatt ttccagctct tctacacttc tgtagtcccg gcgatagtac
     9781 atttcttctt tcaggtgacc aaagaaattc tccattaccg cattatcaag gcagttgccc
     9841 ttgcgcgaca tgctctgcac taaccctttg tctgccaggg cagactgata actttttatc
     9901 cggtaatgcc acccttgatc actgtggagc agcggttttt ctccttctgc aaggctttcc
     9961 agccccttat ttaacatgcg cttcaccagc tcctctgtag gcctgcaggc
//
LOCUS       DAYPHN010000169         9963 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000169 DAYPHN010000000
VERSION     DAYPHN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.169"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..744)
                     /gene="intI1"
                     /locus_tag="ACG0BW_004026"
     CDS             complement(<1..744)
                     /gene="intI1"
                     /locus_tag="ACG0BW_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFK7260927.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWLWFWVFAQHTHSTDPRSGVVR"
     gene            902..1375
                     /gene="dfrA17"
                     /locus_tag="ACG0BW_004027"
     CDS             902..1375
                     /gene="dfrA17"
                     /locus_tag="ACG0BW_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFK7260928.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1506..2294
                     /locus_tag="ACG0BW_004028"
     CDS             1506..2294
                     /locus_tag="ACG0BW_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFK7260929.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2500..2847
                     /locus_tag="ACG0BW_004029"
     CDS             2500..2847
                     /locus_tag="ACG0BW_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFK7260930.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2841..3680
                     /gene="sul1"
                     /locus_tag="ACG0BW_004030"
     CDS             2841..3680
                     /gene="sul1"
                     /locus_tag="ACG0BW_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFK7260931.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3808..4011
                     /locus_tag="ACG0BW_004031"
     CDS             3808..4011
                     /locus_tag="ACG0BW_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260932.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4167..5372
                     /gene="chrA"
                     /locus_tag="ACG0BW_004032"
     CDS             4167..5372
                     /gene="chrA"
                     /locus_tag="ACG0BW_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFK7260933.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5383..5688
                     /locus_tag="ACG0BW_004033"
     CDS             5383..5688
                     /locus_tag="ACG0BW_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFK7260934.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5915..6679
                     /locus_tag="ACG0BW_004034"
     CDS             5915..6679
                     /locus_tag="ACG0BW_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFK7260935.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(7172..7756)
                     /gene="mphR(A)"
                     /locus_tag="ACG0BW_004035"
     CDS             complement(7172..7756)
                     /gene="mphR(A)"
                     /locus_tag="ACG0BW_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFK7260936.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7756..8994)
                     /gene="mrx(A)"
                     /locus_tag="ACG0BW_004036"
                     /gene_synonym="mrx"
     CDS             complement(7756..8994)
                     /gene="mrx(A)"
                     /locus_tag="ACG0BW_004036"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFK7260937.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(8991..9896)
                     /locus_tag="ACG0BW_004037"
     CDS             complement(8991..9896)
                     /locus_tag="ACG0BW_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFK7260938.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2072 a         2880 c         2936 g         2075 t
ORIGIN      
        1 acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc tgcgcaaaaa cccagaacca
       61 cagccaggaa tgcccggcgc gcggatactt ccgctcaagg gcgtcgggaa gcgcaacgcc
      121 gctgcggccc tcggcctggt ccttcagcca ccatgcccgt gcacgcgaca gctgctcgcg
      181 caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc ccttgccctc
      241 ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt gcaaaccctc
      301 actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct cgccttccag
      361 aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc gccgcgacgg
      421 ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct tgccgtagaa
      481 gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc
      541 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
      601 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaaactgta
      661 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
      721 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc
      781 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      841 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttagccatta agggagttaa
      901 attgaaaata tcattgattt ctgcagtgtc agaaaatggc gtaatcggta gtggtcctga
      961 tatcccgtgg tcagtaaaag gtgagcaact actctttaaa gcgctcacat ataatcaatg
     1021 gctccttgtc ggaagaaaaa catttgactc tatgggtgtt cttccaaatc gcaaatatgc
     1081 agtagtgtca aagaacggaa tttcaagctc aaatgaaaac gtcctagttt ttccttcaat
     1141 agaaaatgct ttgaaagagc tatcaaaagt tacagatcat gtatatgtct ctggcggggg
     1201 tcaaatctat aatagcctta ttgaaaaagc agatataatt catttgtcta ctgttcacgt
     1261 tgaagtcgaa ggtgatatca aattccctat aatgcctgag aatttcaatt tggtttttga
     1321 acagtttttt atgtctaata taaattatac ataccagatt tggaaaaaag gctaacaatg
     1381 cgttgcagca ccagtcgctt cgctccttgg acagctttta agtcgcgtct ttgtggtttt
     1441 gctgcgcaaa agtattccac aaagccgcaa cttaaaagct gccgctgaac ttaacgttag
     1501 gcatcatggg tgaatttttc cctgcacaag ttttcaagca gctgtcccac gctcgcgcgg
     1561 tgatcgagcg ccatctggct gcgacactgg acacaatcca cctgttcgga tctgcgatcg
     1621 atggagggct gaagccggac agcgacatag acttgctcgt gaccgtcagc gccgcaccta
     1681 acgattcgct ccggcaggcg ctaatgctcg atttgctgaa agtctcatca ccgccaggcg
     1741 atggcggaac atggcgaccg ctggagctaa ctgttgtcgc tcgaagcgaa gtagtgcctt
     1801 ggcgctatcc ggcgcggcgt gagcttcagt tcggtgagtg gctccgccac gacatccttt
     1861 ccggaacgtt cgagcctgcc gttctggatc acgatcttgc gattttgctg accaaggcga
     1921 ggcaacacag ccttgcgctt ctaggcccat ccgcagccac gtttttcgag ccggtgccga
     1981 aggagcattt ctccaaggcg cttttcgaca ctattgccca gtggaatgca gagtcggatt
     2041 ggaagggtga cgagcggaac gtcgttcttg ctcttgctcg catttggtac agcgcttcaa
     2101 ctggtctcat tgctcctaag gacgttgctg ccgcatgggt atcggagcgt ttgcctgccg
     2161 agcatcggcc cctcatctgc aaggcacgcg cggcgtacct gggtagcgag gacgacgacc
     2221 tagcaatgcg cgtcgaagag acggccgcgt tcgttcgata tgccaaagca acgattgaga
     2281 gaatcttgcg ttgagcggca tgtgcgaaaa gtgcatcgac ccgcgccgag ggcatctgat
     2341 gcctaactcg gcgttcaagc ggacgggctg cgcccgccgc tcaactatgc gttagatgca
     2401 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     2461 gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt
     2521 gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt
     2581 actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt
     2641 tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc
     2701 gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc
     2761 tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag
     2821 tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact
     2881 ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa
     2941 tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga
     3001 ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg
     3061 atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca
     3121 agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg
     3181 atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg
     3241 ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg
     3301 aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc
     3361 cggggatggg atttttcttg agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc
     3421 ttcaaaagct gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg cggaaatcct
     3481 tcttgggcgc caccgttggc cttcctgtaa aggatctggg tccagcgagc cttgcggcgg
     3541 aacttcacgc gatcggcaat ggcgctgact acgtccgcac ccacgcgcct ggagatctgc
     3601 gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac gccagagacc
     3661 gagggttaga tcatgcctag cattcacctt ccggccgccc gctagcggac cctggtcagg
     3721 ttccgcgaag gtgggcgcag acatgctggg ctcgtcagga tcaaactgca ctatgaggcg
     3781 gcggttcata ccgcgccagg ggagcgaatg gacagcgagg agcctccgaa cgttcgggtc
     3841 gcctgctcgg gtgatatcga cgaggttgtg cggctgatgc acgacgctgc ggcgtggatg
     3901 tccgccaagg gaacgcccgc ctgggacgtc gcgcggatcg accggacatt cgcggagacc
     3961 ttcgtcctga gatccgagct cctagggatc gcctcagaaa acggaaaata aagcacgcta
     4021 agccgtaagt aagcgtgctc ctgtgaaagc cacagctaaa actgcgtagt acacatagag
     4081 gtattttttt ctagatttat atcgaatttc gttatcgaat ttcgcttatt attgatcaac
     4141 tcgttgcaga tcatgaaggc gaaagtatga cgaataaccc taccgatgac agcagaccat
     4201 ggtcggtctt tcttattttt ctgcggcttg gattgacatc ttttggcggc cccattgcgc
     4261 acttgggcta cttccgcgcc gaatttgtca cacggcggcg ctggctctcc gaacggagct
     4321 atgctgactt ggtcgcgctt tgtcagttct tgccagggcc tgcaagcagc caggtcggca
     4381 tagcggtagg actgtctcgg gctggataca gcggggcgct ggctgcttgg gctggcttca
     4441 cgctgccgtc tgccatagcc ttgatccttt ttgcgctcgg catctccagc tatggcgatt
     4501 acgtctcgca gggcgcgttg catggcttaa aagtggtggc tgtggccgtg gtcgctcaag
     4561 cagtatgggg catggcgcgt aacctatgca cggatgggct gcgagtcacc atcatggcaa
     4621 ttgctacctg cgtcgtttta cttgtgccgt ccgcgtgggg acaggttggc gtgattgcta
     4681 tcgcaggcat cgcaggccgg ttattgttca agccagcgaa agttgttgag catgaccccc
     4741 tacctatcac ggtcagtcac cgggccggcg tgctttggct ctcgctgttc tttgtcttgc
     4801 tgattggcct gccggtgttg gccgaactga tgccaagtca aaccatggca atggtggatt
     4861 ccttctatcg tgtcggatca ctggtgttcg gcggtggtca cgttgtgctg ccattactgc
     4921 aagccgaagt ggtgccctcc ggctgggtca acaatgaatc ctttctcgcg gggtacgggg
     4981 cagctcaagc ggtgcccggc cctttgttca cgttcgccgc gtttcttggt gcctcgatga
     5041 acaccgcccc gtcgggctgg atcggcggca ttgtgtgtct gctggctatc ttcgcgccct
     5101 cgttcttgct ggtcgtcgga tcaatgccat tttgggagcg tttgcgccgc aatacaggca
     5161 tccaagctgc gctggccggg atcaatgccg ctgtagtcgg cttgctgctg gccgcgctgt
     5221 atcagcctgt atggactagc gccatctttc agccgcaaga cttcggcttg gcattagttg
     5281 cccttgtcgc acttatgttc tggaagctcc cgccgtggct ggttgtcctc ggtagcggtg
     5341 cagctgggtg gctgttgagc gtcgctctgt gaggcacaaa aaatgactga caaagacctc
     5401 tacggcggtt tgatccgcct gcacatcctt caccatgcag ccgaggaacc tgtctttggg
     5461 ctggggatca tcgaagagct acgccgacac ggctacgaga tgagcgctgg caccgtgtac
     5521 ccgatgctgc acggcctgga aaaaaaaggc tatctgacct cacgccacga acgcaccggg
     5581 cgacgtgaac ggcgtgttta tgacatcact gaacaaggca gaactgcact tgctgatgcg
     5641 aaaacaaagg tcaaggagct gttcggagag ttggtagaag gtggttgagt ttttgcttct
     5701 atgtcaggtt gagctatacc ctaactggat tgtcattttc agaagacgac tgcaccagtt
     5761 gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca gaagtgatct
     5821 gcaccaatct cgactatgct caatactcgt gtgggctctg ttgcaaaaat cgtgaagctt
     5881 gagcatgctt ggcggagatt ggacggacgg aacgatgacg gatttcaagt ggcgccattt
     5941 ccagggtgat gtgatcctgt gggcggtgcg ctggtattgt cgctatccga tcagctatcg
     6001 cgaccttgag gaaatgctgg cggaacgcgg catttcggtc gaccatacga cgatctatcg
     6061 ctgggtccag tgctacgccc cggagatgga gaagcggctg cgctggttct ggcggcgtgg
     6121 ctttgatccg agctggcgcc tggatgaaac ctacgtcaag gtgcggggca agtggaccta
     6181 cctgtaccgg gcagtcgaca agcggggcga cacgatcgat ttctacctgt cgccgacccg
     6241 cagcgccaag gcagcgaagc ggttcctggg caaggccctg cgaggcctga agcactggga
     6301 aaagcctgcc acgctcaata ccgacaaagc gccgagctat ggtgcagcga tcaccgaatt
     6361 gaagcgcgaa ggaaagctgg accgggagac ggcccaccgg caggtgaagt atctcaataa
     6421 cgtgatcgag gccgatcacg gaaagctcaa gatactgatc aagccggtgc gcggtttcaa
     6481 atcgatcccc acggcctatg ccacgatcaa gggattcgaa gtcatgcgag ccctgcgcaa
     6541 aggacaggct cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc gccttgtgga
     6601 gagagctttt ggcattgggc cctcggcgct gacggaggcc atgggcatgc tcaaccacca
     6661 tttcgcagca gccgcctgat cggcgcagag cgacagccta cctctgactg ccgccaatct
     6721 ttgcaacaga gcccgccgtg ctagtctgct cggtgatggt ggagtgaagc caacccgcaa
     6781 tcgggttatg aatctgcatc gcgattcgca atcagctgtc tcttgagcat gtcgaactcc
     6841 tgcgatgtta tctcgccgtg ctcgtgctgc tacaccaact tgtccaactc atccgccaca
     6901 ccgctcccca ccacctgcga tggccaggtt gtttgctggg cgcattggcg gagtatcgtc
     6961 tcgacccggg acacccacac cggcacaacc ttacgggagt gattcactgt caaagaatcg
     7021 gcccggtgct ctgacgcaag tatcgggatg gtcaccattt gtaagccgta gacctgagtg
     7081 gtgatcaaga cttcgatacc accgaccgta ccggtactaa tcgacgacgg tcgtgttcgt
     7141 cgcctgccgc agggactctg cacacctccg tttacgcatg tgcctggagg agttggaaat
     7201 cgtcgtgttc gggaaacatt aaacacagga tggcagcgat ctgagccagc acatgatcag
     7261 ctagctcacc atccggatcg acggcccact gcatcgtcgc gccagcgatg accgagtgca
     7321 ggagcaactc agctgccgca ggagcacctg ggggcagtcg cttgcggatc ccctccacca
     7381 ccgcgcggtt ccgctggatc gcaagcgtgc gtagctccgg cacctggagc tcgtaccagg
     7441 agatgagata gttcaccgag aagtcgttgc gagtgttcat gctccgaacg agcacctgca
     7501 aaaattccca gagcccttgc ggccctgcgc ctatcggtat cgcattcagg taatgccgca
     7561 cctgctcgac gccgcgctcc atcatcctca ccagcagcgt atcgcggttg gtgaagcgct
     7621 ggattaacgc tgcgcgggag agccccacct cctttgctac tccgctgagc gtgaactcta
     7681 tgggaccgca acgcttcagc actacggtgg cggcctcgag tacctcgtca tcggacttga
     7741 gcttggggcg gggcatcagt gttcaccttc tgtatgggtt ggggcggagg ctgtggctgc
     7801 cgccgccatt gtagcaaatt gaagacggag cgagagtaga gccacgagcc ccgcaaacac
     7861 ggccgataca acgaggccag ggagcggacc agcaaggtcg acaaacgcgc cggccgcaag
     7921 cataaccatg ggcgaggctg acagcatcac cgccgagacc gtgccgagta cccggccgag
     7981 aagttctggc ggcgtgcggt tgtagatggc agcgttgaga atgggagaga ctgagccggt
     8041 cagcagtccc acgagcgcgc ccaacaacat cagcaccggc acgcctggca actgtgaaag
     8101 cagaagcgag cccaccgcag agccacaaaa tgccaccgcc agccagttct gcgctgatat
     8161 ccgggcgccg accgacgcat gaatggcaat gccaaggaga ccaccagccc ccatcattga
     8221 ggagaacagc ccgagctctg ctacttggcg tcctgcatct acaaacagcg caggcatgat
     8281 gacgctgccg ttggcgccaa cgatgcccac gaagatcatc actataccaa agagagggcg
     8341 cagcaggggt tcgctccaga gaaaagcgac gccggcgcgc atggagagag tcgccgtcgt
     8401 ggtcatcgtc cgagcggcac gcgcgggaag cacccacgcg ccgagcagac ctgcaaggac
     8461 ggagcagaac gccgtcagcc cgagcgttgg cgcagcgcca agcaggccga ttgcggcccc
     8521 cccaagggcc gggccaccta gaatcgcgac gttcccgatc accgctttca gtgacgagac
     8581 gcgctcaacg gagagcccgg cgacgtggcc gagtttgggc agctcactgt cctgcgcggc
     8641 cataccgggt gcgtcgaacg cggcaccgag caccacgcaa gcgatcagcc cagtgttcga
     8701 gagggcgcca acggcatcga gcagtgggat gctcgccatg gccacgccgc ccaccacacc
     8761 cgagatcaat gcgacgggcg cgcgcccgaa ccgatcgacg aggccaccac caacccacgc
     8821 gccgatgatg gtcgcgatga cgctgctagc ggccgtggcg cccgcccagg ccgcgctctt
     8881 tgtatgagac aggacgaacc atggaagcgc gagggccgcc accgcgttgc cgatccggaa
     8941 gagaaaggtc gccgcgaaca gcgtcgcgag cgggctatat cgacgttcgc tcattccgct
     9001 gcggcgagct gcgccttcgc cgcagcgagg tactcttcgt tacccgagtc gagggcgaag
     9061 agtgcgtagg tgaccgcccc gaacgcaagg cgctccgcga tgtggtgggc gagccgcggc
     9121 cacacccggc caccggccgc ttcatacgtg aggaggagct tcgcgagccc ctcttcacca
     9181 aagaccataa ggtgcgcggc catgtcgatg gcagggtcat caacgcgggc ctcgctccag
     9241 tcgatcatcc cgctgacgcg ctccgtgttg tcgatgagca catggcccac gtagagatcg
     9301 ccatgcacca ccacggagaa atctggccac gacgaatcgt cgtcgagcca gcgctgccac
     9361 cggtggaggc gcttgtcgtt caccacgaac tcgcgtcgga cgcggtcaac gtcgtcggcc
     9421 accttctgac gggcctgcgt cggtgtacgg atgagcatcc ccgcatccac ggcggcggaa
     9481 atggggacgg catgcagggc ggcgagcgcg gtcgcgaagc tctccgcgaa gacctccgag
     9541 tcctgcggca cgacccagtc gggcgtggac gaaccaggct ggatgaccat cgcagtcgag
     9601 tcttcgagca tgggataggc aacgagctcg gcgttggcca cgcgccagtc cggcaccgcg
     9661 aacggcaggc gattcttgag cattgccagc acccgcgcct ctggttcgac cttcgcgctt
     9721 acctcggctc ggcgcgggat gcgcagcacc caccgacgtc catcgtcgac ggtggcgatc
     9781 acgatcctat agtcgagccc aagctcattg acagtcagcg ggccatggag cttgagccca
     9841 tgtcgggctg caagtgcgta cagttgggag gtatcggcgg tcgtgactac ggtcatgatt
     9901 cactcctgag ggcttgacgg gtttagccac ctaaatgtaa cagtcacgtc ggttatattc
     9961 aat
//
LOCUS       DAYPHN010000170         9739 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000170 DAYPHN010000000
VERSION     DAYPHN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.170"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..334
                     /locus_tag="ACG0BW_004038"
     CDS             <1..334
                     /locus_tag="ACG0BW_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFK7260939.1"
                     /translation="AGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGL
                     DDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVD
                     GRLVYERN"
     gene            370..1737
                     /gene="ghxQ"
                     /locus_tag="ACG0BW_004039"
                     /gene_synonym="ygfQ"
     CDS             370..1737
                     /gene="ghxQ"
                     /locus_tag="ACG0BW_004039"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFK7260940.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(1773..2261)
                     /gene="ygfS"
                     /locus_tag="ACG0BW_004040"
     CDS             complement(1773..2261)
                     /gene="ygfS"
                     /locus_tag="ACG0BW_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFK7260941.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(2261..4180)
                     /gene="ygfT"
                     /locus_tag="ACG0BW_004041"
     CDS             complement(2261..4180)
                     /gene="ygfT"
                     /locus_tag="ACG0BW_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFK7260942.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFGDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            4616..6064
                     /gene="uacT"
                     /locus_tag="ACG0BW_004042"
     CDS             4616..6064
                     /gene="uacT"
                     /locus_tag="ACG0BW_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFK7260943.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            6066..6191
                     /gene="yqfG"
                     /locus_tag="ACG0BW_004043"
     CDS             6066..6191
                     /gene="yqfG"
                     /locus_tag="ACG0BW_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFK7260944.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(6188..6259)
                     /locus_tag="ACG0BW_004044"
     CDS             complement(6188..6259)
                     /locus_tag="ACG0BW_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260945.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            6314..6862
                     /gene="idi"
                     /locus_tag="ACG0BW_004045"
     CDS             6314..6862
                     /gene="idi"
                     /locus_tag="ACG0BW_004045"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFK7260946.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQMGESNEDAVIRRCRFELGVEITAPEPVYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(6905..8422)
                     /gene="lysS"
                     /locus_tag="ACG0BW_004046"
     CDS             complement(6905..8422)
                     /gene="lysS"
                     /locus_tag="ACG0BW_004046"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFK7260947.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIYCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8432..9530)
                     /gene="prfB"
                     /locus_tag="ACG0BW_004047"
     CDS             complement(join(8432..9454,9456..9530))
                     /gene="prfB"
                     /locus_tag="ACG0BW_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFK7260948.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(9621..>9739)
                     /locus_tag="ACG0BW_004048"
     CDS             complement(9621..>9739)
                     /locus_tag="ACG0BW_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260949.1"
                     /translation="ALWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI"
BASE COUNT         2193 a         2540 c         2440 g         2566 t
ORIGIN      
        1 tgccggaaca actttcaaca tgctgcaaac gctaaacgaa gcctacaaag tgttgcaact
       61 acaaggctat cgcctctcgg catatgaagc gttttacctg gccacgctcg gcggagcgaa
      121 atctctgggc cttgacgatt tgattggcaa ctttttacct ggcaaagagg ctgatttcgt
      181 ggtgatggaa cccaccgcca ctccgctaca gcagctgcgc tatgacaact ctgtttcttt
      241 agtcgacaaa ttgttcgtga tgatgacgtt gggcgatgac cgttcgatct accgcaccta
      301 cgttgatggt cgtctggtat acgaacgcaa ctaataataa aactttaaca tcctcgtgag
      361 gacatcatta tgtctggaga catcctacaa acaccggacg caccaaagcc acagggcgcg
      421 ctggataatt attttaaaat taccgctcgt ggcagtaccg ttcgtcagga agtactggct
      481 ggcttaacga cctttctggc catggtttat tccgttatcg tcgttccggg aatgctgggc
      541 aaagcaggtt ttcctcccgc agctgtgttt gttgccacct gtctggtcgc gggcttcggc
      601 tcgttgctga tgggattatg ggccaatttg ccaatggcga ttggttgcgc gatttccttg
      661 acggcgttta ccgcattcag tctggttctc gggcaacaaa ttagcgttcc tgtcgcactg
      721 ggcgcggtct ttctgatggg cgtcatcttc accgccattt ccgtaaccgg tgtgcgtacc
      781 tggatcttac gtaatttgcc catggggatc gctcacggta caggtatcgg tatcggcctg
      841 tttctgctgc tgattgctgc taacggtgtg ggaatggtta tcaaaaaccc gattgaaggc
      901 ttgccagtgg cgctcggtgc gtttacctcc ttcccggtga tgatgagctt gctggggctg
      961 gcggtcatct tcggtctgga aaagtgtcgc gtacccggcg ggatcttgtt ggtgattatt
     1021 gcaatttcga tcatcggctt aatctttgac ccagcggtga aataccacgg tctggtggcg
     1081 atgccaagcc tgactggcga agatggtaag tctctgattt tcagcctcga tattatgggc
     1141 gcactccagc caactgtact tccgagtgta ctggcattgg tgatgaccgc agtgttcgac
     1201 gccactggca ccatccgtgc cgtcgccggt caggcgaatt tgttggataa agacaaccag
     1261 atcatcaacg gcggcaaagc cctgaccagt gactcagtaa gttcaatatt ctccggcctg
     1321 gtgggcgcag cgcccgcagc ggtttatatc gaatcagcgg caggaaccgc cgccgggggt
     1381 aaaacagggt taaccgcaac cgtagtgggg gcgttattcc tgctgattct gttcttatca
     1441 ccgctgtcat ttttgatccc tggttacgcc actgcacccg ctctgatgta cgtaggtttg
     1501 ctgatgttaa gtaacgtctc gaagctggat ttcaatgatt ttattgacgc tatggctggc
     1561 ctggtgtgtg ccgtgttcat cgttctgact tgtaatatcg ttaccggtat tatgctgggc
     1621 tttgtgacac tggtcgtagg ccgcgtcttt gcacgcgaat ggcaaaagct gaatattggt
     1681 acggtgatca ttactgccgc actggtcgca ttttacgcgg gtggttgggc aatctaatag
     1741 attctccgcg cctttcagcg cggagatttc tttcacagag gattctcacc gctggcaaca
     1801 atacgctgct ggcggactcg cctgagttct ctttcagtaa gcaactgcag cgcctgtgtt
     1861 gggcaagatt caacgcaggc aggcccctct tcccgctgtt cacagagatc gcatttcacg
     1921 atttgttgcc gggtatcccc cggcaacgac tgaatggtga tcattccaaa cgggcacgca
     1981 ctcacgcaac tctgacagcc aatacaacgg gcagaattgg tctgcaccac ctgctcgccc
     2041 atcgtcagcg cccccacggg gcaagcgcta acacaagggg cgttttcaca ctgatggcac
     2101 atcaccggcg cactgatgct gtccagtcgc tgtaccttca gccggggtaa aaagacatcg
     2161 gcattcaact cctgttctga agggtgagcg accacacagg ctacttcaca agtacggcag
     2221 ccaatacagt cagccggatt aacgataatt aacgatttca tcacgatgcc ttcgtatcaa
     2281 acagagttaa catatcgcgc gccgcctgcc ttcctgcggc cattgcagtg acaaccagat
     2341 ccgcgccatg aacagcatca cccccagcaa agactttttt cagatgcgtc tgggtgggta
     2401 aatacccgac gtcgccggtt tgaatcaggc cccatttatc gagtttaatt ccgctgccct
     2461 gcaaccacgg catggcatgc gcctggaaac caaaggccat aatgagaaca tcggcgggca
     2521 attcaaactc tgaacccgct accggacgag gacgacggcg accatccggc cccggctcac
     2581 ccatggcggt acgaatcagg cccaccgcag ttaaacgccc atcttcgtca caagcgatat
     2641 attgcggctg aacattgaat tgaaactcga caccttcctc gcgcgcattg accacctctt
     2701 tgcgcgagcc cggcatactg acttcatcac gacgatacgc gcaggtcacg ctggcagcat
     2761 tgaggcgaat ggaagtccgc aaacaatcca tggtcgtatc gccaccgccc aataccacga
     2821 ctcgcttacc ttccacatcc gtcagcggat actcttcaga ctccggcaac cccatgagct
     2881 ggcgggtatg ggcagtcaga aacggtagag cctgaatgac accgggcgca tcttcatgcg
     2941 gcagatctgc tcgcatcatc ccgtaagtcc ccacgccgat gaaaactgca tcatattcag
     3001 aagttaaatc gccaaaggta atatcgcggc caatttcaca gttaagatga aagtcgattc
     3061 ccattgcggt gaatatctct cgccgctggc ttaataccgt tttatcgagt ttgaaaggag
     3121 gtatgccaaa agtcagcata ccgccaattt ctggatggcg atcaaagaca tcaacctgaa
     3181 ctcctgcgcg cgccaaaata tcagcacacc ctaaccctgc aggccctgcg ccaatcaccg
     3241 ccactttttc gatacgggga acaactttgc tgacatcggg acgccagccc atcgccagcg
     3301 cggtatcggt gatgtagcgt tccagattcc cgatagagac tgcgccagag tgatctttca
     3361 aagtacatgc gccttcacaa agacggtcct gtggacatac cctgccgcag atttctggta
     3421 aggaactggt ctggtggcaa agttctgccg cttcaataat ctttccttcc tgcaccagac
     3481 ggatgtaatc cggaatagcg ttatgcagcg ggcaatgcca gttgcagtta gctttttcgg
     3541 cgcaataaac acagcggtca ctctcataag tcgcttgttg tggatccagc ccgtaataga
     3601 tttcgccaaa gtgggttttc cgttcactcg ctgaaatttt atctgcgcct ttacgcgagt
     3661 taacggggag caacgctgca ctgcgagatg gctgagcgtc tgatgacgct tttcctgctg
     3721 ccgttttgcg ctggcgggcc acctttatct gctgcaaccc tttatcgtcc atcagccgta
     3781 acgcctgtgt tgggcagact tcaatacagg cttgcgtgcc ggaactgcgc tggttacaaa
     3841 ggtcgcattt ctgcgcaatc gtatcgacca tctcaacgac gccaaagggg caagcgattg
     3901 cgcatctttt acaaccaata catttttgct cgtccagttg tacgctatcg ggctggaaag
     3961 tcagagcatt aaccggacaa gccgtaacgc aaggggcatt gttgcagtga tggcaggcca
     4021 ccggattcgc agcctggcct ttccctacaa cgtggatacg cggtcgaaag tcactgtgac
     4081 tcagcggcca gttttcttga ttatgtgcca tcgcacaggc aatttcacaa gcatgacagc
     4141 ctatacattc cgcagcttca gcagcgataa acttattcat ttgcatccct ttcatttaat
     4201 gagttatgtc ttctcaaatt tcgcgatgca ataaatgggc atatattagg tagcatggcc
     4261 gatttaatta taattttgcg actgagttca aattattgcc cctcaatctg tgccgctttc
     4321 attatttcaa cattgccgac caaaagagat ctaaccagat aaaaacgatt aaatacgaga
     4381 gtgcgacatg ccagaatgat taatgaaata tcaatatgag aaaattacag ataattccat
     4441 tgtcaggttc tcttctcatt ttcgtggttt aaccacgatt atgtgattgc ctccgcaatt
     4501 cctgtctcta actccccttc ctcgcaaaaa ctggcactcc acgagcatgt gtttagacag
     4561 tttcattaac gtaaacggtt gctttttact ctggcgggcg aaaggagaaa cactgatgag
     4621 cgccatagat tcccaacttc cctcatcttc agggcaagac cgcccaactg atgaggttga
     4681 ccgcatatta tcaccaggaa agctgatcat actcggtctg caacacgtcc ttgtcatgta
     4741 cgcaggtgca gtcgctgttc ctcttatgat tggtgaccga ctgggcctct caaaagaagc
     4801 tattgcgatg ctcattagct cggatctctt ttgctgcggg atcgtcacat tattgcaatg
     4861 tatcggtatc ggccgcttta tggggatccg cctgccggtg attatgtcgg tgacctttgc
     4921 tgctgtaaca ccaatgatag ccattgggat gaacccggat atcggcctgc tggggatatt
     4981 tggtgccact atcgccgcgg gttttatcac cacattatta gcgccactta tcggtcgctt
     5041 gatgccttta ttcccgccac tggttaccgg tgtggttatt acttctatcg gacttagcat
     5101 cattcaggtg ggtattgact gggccgcagg aggtaaaggg aatccgcaat atggtaatcc
     5161 cgtttattta ggtatctcct ttgccgtctt aattttcatc ttgctcatta ctcgctatgc
     5221 gaaaggattt atgtccaacg tcgccgtatt actggggatt gtatttggct ttttactttc
     5281 gtggatgatg aatgaagtca atttatccgg gctacatgat gcctcgtggt ttgcgattgt
     5341 tacgccgatg tcgtttggta tgccgatttt tgatcccgtt tccattctga ccatgactgc
     5401 cgtgttaatc atcgtgttta tcgagtcgat ggggatgttc ctggcactgg gtgaaatagt
     5461 cggtcgtaaa ctctcttcac acgatattat tcgcgggctg cgtgtcgatg gcgtagggac
     5521 aatgataggc ggaacgttta acagctttcc ccacacgtca ttttctcaaa acgttggcct
     5581 ggttagcgtg acgcgcgttc atagccgctg ggtgtgtatt tcttcgggaa ttatattaat
     5641 cctgtttggc atggtgccaa aaatggcggt gctggtcgcc tccattccgc aatttgtgct
     5701 gggcggtgct gggctggtga tgttcggcat ggtactggcg acagggattc gaattctgtc
     5761 gcgctgtaac tacaccacca atcgttacaa cctctatatt gtggcgatca gtctcggcgt
     5821 tggcatgact ccgacgctct ctcacgattt cttttctaag ttaccggccg tactgcaacc
     5881 gttgctgcat agcggcatta tgctcgcaac ccttagcgcc gttgtgctga acgtcttctt
     5941 taatggctat cagcatcatg ctgatctggt gaaggaatcc gtctctgata aagatttaaa
     6001 agtcaggaca gtacgtatgt ggcttctgat gcgcaagctg aagaaaaatg agcatggaga
     6061 ataatatgaa tcttttaatg cgcgctatat tcagtctgtt gttgcttttt actctctcta
     6121 ttcctgtcat ttctgactgt gttgcaatgg ccattgaaag tcgcttcaaa tatatgatgc
     6181 tgctttttta aatggttttt acctgtcgac atccgctcaa aacgggcggt tgtcgataaa
     6241 cgctcacttg gttaatcatt tcactcttca attatctata atgatgagtg attagaatta
     6301 catgtgagaa attatgcaaa cggaacacgt cattttattg aatgcacagg gagttcccac
     6361 gggtacgctg gaaaagtatg ccgcacacac ggcagacacc ctcttacatc tcgcgttctc
     6421 cagttggctg tttaatgcca aggggcaatt attagttacc cgccgcgccc ttagcaaaaa
     6481 agcgtggccg ggcgtgtgga ctaactcggt ttgtgggcac ccacaaatgg gcgaaagcaa
     6541 cgaagacgcg gtgatccgcc gttgccgctt tgaactgggt gtggaaataa cagcaccaga
     6601 accggtctat cctgactttc gctaccgcgc caccgatccg aatggcattg tggaaaatga
     6661 agtgtgtccg gtatttgccg cacgcaccac cagtgcgtta cagatcaacg atgatgaagt
     6721 gatggattat caatggtgtg atttagcaga tgtattacgt ggtattgatg ccacgccgtg
     6781 ggcgttcagt ccgtggatgg tgatgcaggc agccaatagt gaagcaagaa aattgttgtc
     6841 tgctttcgcg cagcacaatt aaaaaacccc gacatttgcc ggggttgtga gcataacgta
     6901 atgcttattt caccggacgc atcgccggga acagaataac gtcgcggatg gtatggctgt
     6961 tggtgaacag cattaccata cggtcgatac caattcccag acctgctgtc ggcggtaagc
     7021 catgttccag tgcggtgacg taatcttcgt cgtagaacat cgcttcgtcg tcgcctgcgt
     7081 ctttcgcggc aacctgatcc aggaagcgct gcgcctgatc ttccgcgtca ttcagctcgc
     7141 taaagccgtt accgatttca cgaccaccga taaagaactc aaagcggtca gtgatttccg
     7201 ggttaacgtc gttacgacgc gccagcggag aaacttctgc cggatattca gtaatgaagg
     7261 tcggctgaat cagatgtgct tctgccactt cttcgaagat ctcggtaacg atacggccca
     7321 gaccccagct cttctcaacg tggataccga tagattcagc aatcgctttc gcagagtcga
     7381 agttgtccag atctgccatg tcggtttccg ggcgatattt cttgatcgct tcacgcatgg
     7441 tcagtttttc gaacggctta ccgaagtcca gcgttacgtc gccgtaggtc acttccgtct
     7501 taccgagaat atcctgtgcc agagtacgga acagcgattc ggtcagctcg ataagatctt
     7561 tgtaatctgc gtaagccata tagagttcca tcatggtgaa ctctgggtta tgacgtacgg
     7621 aaataccttc gttacggaag ttacggttga tttcgaatac acgctcgaag ccaccgacca
     7681 ccagacgctt gaggtacagt tccggcgcga tacgcaggta catgtcgaga tccagcgcat
     7741 tatggtgggt gataaacgga cgcgcagcgg caccgcctgg gatcacctgc atcatcggcg
     7801 tttcaacttc cataaagccg cggttcacca tgaactggcg aataccagag aggatctgcg
     7861 agcgcacttt aaaggtgttg cgggattcat cgttagagat gagatccaga taacgctgac
     7921 gatagcgcgc ttcctgatcc tgcaagccgt ggaatttgtc cggcagcgga cgcagtgctt
     7981 tggtcagcag acgcagctcg gtgcagtaga tagacagttc gccggttttg gttttgaaca
     8041 gtttaccttt cgcgccgagg atgtcgccga ggtcccattt tttgaactgc tcgttgtaaa
     8101 cgccttccgg cagatcgtca cgggcaacgt acagctgaat gcggccaccc acatcctgca
     8161 gggtaacgaa agacgcttta cccataatac ggcgggtcat catgcggcca gcaacggcga
     8221 cttcgatgtt cagcgcttcc agttcttcgt tctctttacc gtcgaattct gcgtgcaatt
     8281 ggtcagaggt atgatcgcga cggaaatcgt tcgggaaggc aatcccctgc tcgcgcaagt
     8341 tcgccagctt ctcacgacgc gttttcagtt cattgttaag atcgactacc gcgtcagcgc
     8401 cctgtgcgtg ttgttcagac atgttggttc ctcataaccc tgctttcaaa cttgcttcga
     8461 taaattgatc caggctgccg tccagcacgg cctgcgtgtt acgggtttct accccggtac
     8521 gcagatcttt aatgcgggag tcatcaagga cataagaacg aatctggctg ccccagccga
     8581 tgtcggattt gttatcttcc atcgcctgct tctcggcatt tttcttctgc atctccagtt
     8641 cataaagctt cgctttcatc tgcttcatgg cctggtcttt gttcttgtgc tgggaacggt
     8701 cgttctggca ctgggtcacg atcccagttg ggatgtgagt aatacgcacc gcagattcgg
     8761 tacggttaac gtgctgaccg cccgcgccgg acgcgcgata aacgtcgatg cgcagatccg
     8821 ccggattgat ttcgatatcg atatcgtcat caacttccgg gtaaacaaac gcggagctga
     8881 acgacgtgtg gcgacgaccg ccggagtcaa acgggctctt acgcaccagg cggtgaacgc
     8941 cagtttctgt acgcagccag ccgtaagcgt aatcgccgga gattttgatc gtcacggatt
     9001 taatacccgc cacttcacct tccgactctt cgatgatttc agttttgaaa ccacgcgatt
     9061 ctgcccagcg cagatacata cgctcaagca tgctcgccca gtcctgtgct tccgtaccgc
     9121 cagaccccgc ctgaatatcg aggtagcagt cggcgctgtc atattcgcca gagaacatac
     9181 ggcggaactc aagctgcgcc agtttttctt ccagagcgtc gagttcagca acggcttcgt
     9241 taaaggtttc ttcgtcgtca gcttctacag ccagttccag cagaccagaa acatcttcca
     9301 gcccctgttt catttggtca agggtgtcga caacggcttc gagggaggaa cgctctttac
     9361 ccagcgcctg tgcgcgttca ggttcgttcc agacatccgg ctgttccagc tcggcgttta
     9421 cttcttccag acgctctttc ttggcgtcat agtcaaagat accccctaag aacgtcggag
     9481 cgttccgtga ggtcctgaat gcgattattt accggattaa tttcaaacat ggtctgattt
     9541 cttttattga gctagtcaaa atgcggtgat aagagcggga ttgtacccaa tccacgctct
     9601 tttttataga gaagatgacg ctaaattggc cagatattgt cgatgataat ttgcaggctg
     9661 cggttgccgc gaaactcgtt gatatcgagc ttgtaagcca gttgcacttc gcgcacgccg
     9721 ttatccggcc agagggcgg
//
LOCUS       DAYPHN010000171         9653 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000171 DAYPHN010000000
VERSION     DAYPHN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.171"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            84..533
                     /gene="alaE"
                     /locus_tag="ACG0BW_004049"
     CDS             84..533
                     /gene="alaE"
                     /locus_tag="ACG0BW_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFK7260950.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(570..914)
                     /locus_tag="ACG0BW_004050"
     CDS             complement(570..914)
                     /locus_tag="ACG0BW_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFK7260951.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1066..1395
                     /locus_tag="ACG0BW_004051"
     CDS             1066..1395
                     /locus_tag="ACG0BW_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFK7260952.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1452..2786)
                     /locus_tag="ACG0BW_004052"
     CDS             complement(1452..2786)
                     /locus_tag="ACG0BW_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFK7260953.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEVALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITCGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVLPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            2874..3305
                     /locus_tag="ACG0BW_004053"
     CDS             2874..3305
                     /locus_tag="ACG0BW_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFK7260954.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3514..3759
                     /gene="nrdH"
                     /locus_tag="ACG0BW_004054"
     CDS             3514..3759
                     /gene="nrdH"
                     /locus_tag="ACG0BW_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFK7260955.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDHVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3756..4157
                     /gene="nrdI"
                     /locus_tag="ACG0BW_004055"
     CDS             3756..4157
                     /gene="nrdI"
                     /locus_tag="ACG0BW_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFK7260956.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRVLLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQP"
     gene            4139..6283
                     /gene="nrdE"
                     /locus_tag="ACG0BW_004056"
     CDS             4139..6283
                     /gene="nrdE"
                     /locus_tag="ACG0BW_004056"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFK7260957.1"
                     /translation="MATTTVERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDENVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYAEL
                     VANERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6293..7252
                     /gene="nrdF"
                     /locus_tag="ACG0BW_004057"
     CDS             6293..7252
                     /gene="nrdF"
                     /locus_tag="ACG0BW_004057"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFK7260958.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDNPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKSQEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7609..8811
                     /gene="proV"
                     /locus_tag="ACG0BW_004058"
     CDS             7609..8811
                     /gene="proV"
                     /locus_tag="ACG0BW_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFK7260959.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8804..>9653
                     /gene="proW"
                     /locus_tag="ACG0BW_004059"
     CDS             8804..>9653
                     /gene="proW"
                     /locus_tag="ACG0BW_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFK7260960.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIAS"
BASE COUNT         2352 a         2464 c         2436 g         2401 t
ORIGIN      
        1 tatgcgaaaa tgcagatggc aatgagatcc actgctttca tctccattca catcccatta
       61 cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg
      121 cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc
      181 tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca
      241 tcttaattgc atggccatac ggtatgtacc gcgatctgtt tatgcgcgca gcacgcaaag
      301 ttagcccgtc gggctggata aaaaatctgg cggatatcat ggcttatgtg acgttccagt
      361 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
      421 cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
      481 attattgccg ccgactgttt aaagttagcc gttaccagca ggtaaaagcc tgacccttct
      541 tttcgcgact ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa
      601 taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcatgacgt
      661 tcacccgcta aatagagcgg aaattgttga taatgatcgc aagttttaat atcggaagcg
      721 ggttcagcca gtgttgaact acgttctttt aatgttggat gaatcaccag ggcggtacgt
      781 cccattcgcg cttcgcgatt aacatagaca taattttccc cacggcggta accataagct
      841 ttctgggtta ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct
      901 ggtcgtaaat acatatttga tcctcgttat tatcaaccgc gggcctacct tacctgattg
      961 cgcattcaca acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat
     1021 tatggtctac actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg
     1081 aaccgcaacg acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct
     1141 gacagcctcg agtctgtgtt gaagtcctgg ggcagcgacg ccaaagggga agcagaagcc
     1201 gctcgcagta aagcgcaggc tctactgaaa gaaaccaggg cgcgaatgca tggtcgtact
     1261 cgcgtccagc aagccgcacg cgatgccgtt ggctgcgcgg attcttttgt tcgtgaaaga
     1321 ccctggtgta gcgtgggtac agcagctgcg gtaggtattt ttatcggcgc actgttaagc
     1381 atgcgcaaat cgtagtgcaa aaatgataat aaatacgcgt ctttgacccc gaagcctggc
     1441 ttcggggttt tttagcttaa ggctttacgt aattgttgtg ctacctgtcg cgccatacct
     1501 tcgttaacga tattagtaaa cgagagcagt aatccacctt ccccacttga acggatacgc
     1561 cagtcgctaa gtgcctgcac cgcaaggcct gcagcattgg ctttacgcgc atgggcaata
     1621 tcatcgccta tcattctgat caccatctgg ataccacctt tctgcggcac aacctgaaat
     1681 ccttgctgcg tcaacgcttg ctcaatccat tgccgacgtt gagcataatg ctgacgcatt
     1741 ttcttcagat gccgccagaa atgcccctcg cgaagaaagt ctgccagtgt gttctgccat
     1801 agaacaggta ccgcacatgg agccagtgac gcctggtggc ggaattgtgc aatttgcttc
     1861 accggcaaca ccagccacgc acagcgcaat gcaggaaata gcgctttgct gaatgttccg
     1921 gcataaatta cccgctgcgg tgcgtcgaga ctttttaacg ccggtaacgg cttaccgtga
     1981 tagcgaaact cactgtcata atcatcttca ataatccatg cctgactacg atctgcccat
     2041 tccagtatct gatgcctgcg cgctaaagag agcgccacac ccagcggact ttggtgtgct
     2101 ggtgttatca gggcaaaacg cgcatcagga taattttgta ttccgcatgt gatatccagt
     2161 ccattgtcat caaccggcac aggcaaaatt tccacatcat ggcgagtgac aatcgggcga
     2221 atcagtggaa agccgggatc ttctatccac atcccgtttc ccggtttcgc cagcgcgtgc
     2281 agaattaaag ctattgaggc cgcataaccg tgagtgataa agacctgctc tggctgacaa
     2341 tcaatcccac gtgaaacgcg taagtaatca actattgcct cgcgcaacgc cacctcaccg
     2401 cagacatcgc ctaacgccaa atcaaagcgc gtctgggtac gaaggcgacg ccccatcacc
     2461 cgcgcccaca gctctcgcgg aaagagatcg agtgcgggta atcccatttg aaatggcaac
     2521 ggttgctgac tttcaccggc aaaagctaca acttgtacag tagattgttg cggatatatc
     2581 cgctcactga caaatgtgcc agcctgtccc cgcctgatca gccatccttg cgccaccagc
     2641 tcagcatacg cattttcgac agtggatcga gacaccccca gctcctgcga ccaggtacga
     2701 cttgaaggca gccttgcgcc gggtttcagt tccccttgct caatagccgt ttttaactga
     2761 cgggcgatat cctgatagcg cggcatatgg tctgcttttt taggtaattt atggctctct
     2821 ttagcagtcc attatagtgc tacattgcgg gcgttaataa gaggagaaac tgtatgacta
     2881 cattacgtca gccttactac gaacttagcc cggcagtgta taacgcacta gtgcaggcca
     2941 aaacggcact ggaaaatagc acgctggata ccacgttgat ggagttagtt tatttgcgcg
     3001 tctcgcaaat caacggctgc gcattttgtc tggagatgca cagcaaagca ttgcgcaaat
     3061 ccggcgtgcc acagcacaaa ctggacgccc tggcaggttg gcgcgtaagc catcattttg
     3121 atgaacgcga gcgggcggcg ctggcatggg cggaatcggt aaccgaaatt gccagaaccc
     3181 atgcggaaga cgaggtttat cagcctttgc ttgagcattt cagcgcagca gagatcagcg
     3241 acttaacgtt tgccatcggg ctgatgaatt gttttaaccg tctggccgtt tccatgcgga
     3301 tgtaacttct tgatgccggg tattcacccg gcaaaaaaac acaaaatccc ctaccccgtt
     3361 gagccattca tcggtggcgt aatcaatcta aaattcccat atcttgtatg gttgaatctt
     3421 ttttcaacta catctagtat ctctgtatca acagagagac aacccgacgc gtatcatcgc
     3481 gccgtatctt cattttaaac ggaaatacga atcatgcgca ttactattta cactcgtaac
     3541 gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accggggctt tgattttgaa
     3601 atgattaatg tcgatcacgt tcctgaagcg gcggaagcgt tgcgtgctca gggctttcgt
     3661 cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgccc ggacatgatc
     3721 aatcgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc tacttttcca
     3781 gtagctccga aaatacgcag cgttttatcg aacgtttagg actgcccgcg gtgcgcatcc
     3841 cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc gtgccctctt
     3901 acggcggcgg cggtacggct ggcgcagtgc cacgacaggt cattcgcttt ttaaacgacg
     3961 agcacaaccg ggtgttgctg cgcggcgtta ttgcttcggg taatcgcaac tttggtgagg
     4021 cgtatggccg cgccggagat gtgatagccc ggaaatgcgg cgtgccgtgg ctgtaccgct
     4081 ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta accgaatttt
     4141 ggcaacgaca accgtagaac gcctgacgca ggaaacgatg gattaccacg cgctgaatgc
     4201 gatgcttaac ctttacgata gcgcaggtcg cattcagttc gataaagatc gccaggccgt
     4261 tgacgccttt atggcgacac atgtgcgtcc gaacagtgtg accttcagta gccagcagca
     4321 gcgcctgaac tggctggtca acgaaggtta ctatgatgaa aacgtcctca accgctactc
     4381 tcgcgatttt gtcattacgc tgtttgccca cgcgcacgcc agcggttttc gtttccagac
     4441 attcctcgga gcatggaagt tttacaccag ctatacgttg aagacattcg acggtaagcg
     4501 ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg cacaaggcga
     4561 tgaaactctg gcgacgcaac tgaccgatga gatgctgtca ggacgctttc agccagccac
     4621 gccaacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct gttttttgct
     4681 gcgtattgaa gacaatatgg agtcgattgg tcgggcggta aattccgcac tgcaactgtc
     4741 gaaacgcggc ggcggcgtag catttttgct gtcgaatctg cgagaagcgg gcgcgccaat
     4801 taaacgtatt gaaaatcaat catctggcgt gattccggtg atgaaaatgc tggaagacgc
     4861 attttcctat gccaaccagc tcggcgcgcg tcagggtgct ggcgcagtct atttacatgc
     4921 ccatcatccc gatattctgc gttttctcga tacgaaacgg gaaaatgccg acgagaaaat
     4981 ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc atctggcaaa
     5041 agagaatgcg cagatggcac tgttttcgcc ttatgacgtt gagcgggttt atggcaagcc
     5101 gtttgctgat atcgccatca gtgaacacta tgccgaactg gttgccaatg aacgcatccg
     5161 caaaaaatac ctcaacgccc gtgatttctt ccagcgactg gcagaaatcc agtttgagtc
     5221 cggctatccc tacatcatgt atgaagacac ggttaaccgt gctaacccta tcgccgggcg
     5281 cataaatatg agcaatctct gctcagaaat tttgcaggtt aacagcgcct cagagtatga
     5341 cgagaatctc gactatgccc gcacaggcca tgatatttcc tgcaatttag gttcgttgaa
     5401 tattgcccac accatggatt cccccgactt tgcccgcacg gtagagactg ccgtgcgcgg
     5461 tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg aagccggaaa
     5521 tgccgcctca cacgccatcg gactggggca gatgaattta cacggctatc tggcgcgaga
     5581 aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt tctataccat
     5641 cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg aaaccttcgc
     5701 cgggttcaaa cagtcacgct atgccagtgg tgaatatttt agccaatatc tgcaagggaa
     5761 ctggcagccg aaaacggcga aagttggcga actgtttacc cgtagcggta ttacgttacc
     5821 tacccgtgag atgtgggcgc agctgcgcga cgacgtgatg cgctacggca tttacaacca
     5881 gaatcttcag gcggtgccgc ccaccggttc tatctcttat atcaaccatg ctacgtcgag
     5941 tattcatcca attgtggcga aagtagagat acgcaaagag ggcaaaacag ggcgcgttta
     6001 ctaccctgcc ccgtttatga ctaacgaaaa tctggcgctg tatcaggacg cttacgaaat
     6061 cggcgcagaa aagatcatcg acacctacgc ggaagcgact cgccatgtcg atcaggggct
     6121 gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca aagcgcagat
     6181 ttacgcctgg cgcaagggta tcaaaacgct ctactacatc cgcctgcgtc agatggcgct
     6241 ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat ctatgaaact
     6301 ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc tggaggtgtg
     6361 gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga acgatattcc
     6421 tgcatggcag acattaagtg tcgtagaaca acaactgacg atgcgcgttt ttactggcct
     6481 gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc ccgatgcgct
     6541 cacgcctcat gaagaggcgg tattatcgaa tatcagcttt atggaagcgg ttcatgcccg
     6601 ctcttacagt tcgattttct cgacgctgtg ccagaccaaa gatgtcgatg ccgcctacgc
     6661 ctggagtgaa gaaaacgcgc cgttgcagcg aaaagctcag attattcagc aacattatcg
     6721 cggtgataat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt ttttgttcta
     6781 ttccggtttc tggctaccga tgtatttttc cagccgcgga aagctgacca ataccgcaga
     6841 cctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatcg gctataaata
     6901 tcagaaaagc caggaaaaga tctcccaggc acaacgtgaa gagttgaaga gtttcgcctt
     6961 cgatttgttg ctggaacttt atgacaacga gttgcaatac accgatgagc tgtacgccga
     7021 aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata aggcgttgat
     7081 gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc cggcaatcct
     7141 cgccgcgctt tcgccgaatg ccgatgaaaa tcatgatttc ttttccggtt caggctcttc
     7201 atatgtgatg ggaaaagcgg tcgaaacaga agatgaagac tggaatttct gagggtgtta
     7261 tttttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata gctttatatc
     7321 acgcaaataa tttgtggtga tctacactga tactctgttg cattattcgc ctgaaaccac
     7381 aatattcagg cgtttttttg ctatctttga caaaaaatat caacttttct cgatttgctc
     7441 tcagccctta tatcatggga aattccggcg atttgctcac atcaatattc atgccacatt
     7501 tgccatcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt gactatttcc
     7561 attgggtaat gtatcgacat aaacaaataa caggaatctt tctattgcat ggcaattaaa
     7621 ttagaaatta aaaatcttta taaaatattt ggtgagcatc cacagcgagc tttcaaatat
     7681 atcgaacaag gactttcaaa agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta
     7741 aaagacgcca gtctggccat tgaagaaggc gagatatttg tcatcatggg attatccggt
     7801 tcgggtaaat ccacaatggt acgccttctc aatcgcctga ttgaacccac ccgcgggcaa
     7861 gtgctgattg atggtgtgga tattgccaaa atatccgacg ccgaactccg tgaggtgcgc
     7921 agaaaaaaga ttgcgatggt cttccagtcc tttgccttaa tgccgcatat gaccgtgctg
     7981 gacaatactg cgttcggtat ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa
     8041 gcccttgatg cactgcgtca ggtcgggctg gaaaattatg cccacagcta cccggatgaa
     8101 ctctctggcg ggatgcgtca acgtgtggga ttagcccgcg cgttagcgat taatccggat
     8161 atattattaa tggacgaagc cttctcggcg ctcgatccat taattcgcac cgagatgcag
     8221 gatgagctgg taaaattaca ggcgaaacat cagcgcacca ttgtctttat ttcccacgat
     8281 ctcgatgaag ccatgcgtat tggcgaccga attgccatta tgcaaaatgg tgaagtggtg
     8341 caggttggta caccggatga aattcttaat aatccggcga atgattatgt ccgtaccttc
     8401 ttccgtggcg tcgatattag ccaggtgttc agtgcgaaag atattgcccg tcgtacaccg
     8461 aacggcttaa ttcgtaaaac ccccggcttc ggcccgcgtt cggcactgaa attattgcag
     8521 gatgaagatc gcgaatatgg ctacgtcatc gaacgcggta ataagtttgt cggcgctgtg
     8581 tctatcgatt cgcttaaagc cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt
     8641 gatgcgccgt tagcggtcga tgcacaaacg cctcttagcg agttgctctc tcatgtcgga
     8701 caggccccct gtgcggtgcc cgtggtcgac gaggaccaac agtatgtcgg catcatttcg
     8761 aaaggaatgc tgctgcgcgc tttagatcgt gagggggtaa ataatggctg atcaaaataa
     8821 tccgtgggat accacgccag cggcggacag tgccgcacag tccgcagacg cctggggtac
     8881 accggcgact gcaccgactg acggcggtgg tgctgactgg ctgaccagta cgccagcgcc
     8941 aaacgtcgag cattttaata ttctcgatcc gttccataaa acgctgatcc cgctcgacag
     9001 ttgggtcact gaagggatcg actgggtcgt tacccatttc cgtcccgtct tccagggcgt
     9061 gcgcgttccg gttgattaca tcctcaacgg tttccagcaa ttgctgctgg gtatgcccgc
     9121 tccggtggcg attatcgttt tcgctctcat cgcctggcaa atttccggtg tcggaatggg
     9181 cgtggcgacg ctggtttcgc tgattgccat cggcgcaatc ggtgcctggt cacaggcaat
     9241 ggttactctg gcgctggtgc taaccgccct gctgttctgt atcgtcatcg gtttgccgtt
     9301 ggggatctgg ctggcgagaa gtccacgagc ggcgaaaatt attcgtccac tgcttgatgc
     9361 catgcagacc acgccagcgt ttgtctacct ggtgccaatc gtcatgctgt ttggtatcgg
     9421 taacgtgccg ggcgtggtgg tgacgatcat ctttgcgctg ccgccgatta tccgtctgac
     9481 gattctggga attaaccagg ttccggcgga tctgattgaa gcctcgcgct cattcggtgc
     9541 cagcccgcgc cagatgctgt tcaaagttca gttaccgctg gcgatgccga ccattatggc
     9601 gggcgttaac cagacgctga tgctggccct ttctatggtg gtcatcgcct cga
//
LOCUS       DAYPHN010000172         9515 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000172 DAYPHN010000000
VERSION     DAYPHN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.172"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            112..1203
                     /gene="rutA"
                     /locus_tag="ACG0BW_004060"
     CDS             112..1203
                     /gene="rutA"
                     /locus_tag="ACG0BW_004060"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFK7260961.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            1203..1895
                     /gene="rutB"
                     /locus_tag="ACG0BW_004061"
     CDS             1203..1895
                     /gene="rutB"
                     /locus_tag="ACG0BW_004061"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFK7260962.1"
                     /translation="MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            1907..2293
                     /gene="rutC"
                     /locus_tag="ACG0BW_004062"
     CDS             1907..2293
                     /gene="rutC"
                     /locus_tag="ACG0BW_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFK7260963.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYSEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            2301..3113
                     /gene="rutD"
                     /locus_tag="ACG0BW_004063"
     CDS             2301..3113
                     /gene="rutD"
                     /locus_tag="ACG0BW_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFK7260964.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIKRYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTHPETFNALLLNGLASLLHHREAACKELL"
     gene            3110..3700
                     /gene="rutE"
                     /locus_tag="ACG0BW_004064"
     CDS             3110..3700
                     /gene="rutE"
                     /locus_tag="ACG0BW_004064"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFK7260965.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            3711..4205
                     /gene="rutF"
                     /locus_tag="ACG0BW_004065"
     CDS             3711..4205
                     /gene="rutF"
                     /locus_tag="ACG0BW_004065"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFK7260966.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            4226..5548
                     /gene="rutG"
                     /locus_tag="ACG0BW_004066"
     CDS             4226..5548
                     /gene="rutG"
                     /locus_tag="ACG0BW_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFK7260967.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQ"
     gene            complement(5637..5810)
                     /gene="ymdF"
                     /locus_tag="ACG0BW_004067"
     CDS             complement(5637..5810)
                     /gene="ymdF"
                     /locus_tag="ACG0BW_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFK7260968.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            6183..6779
                     /gene="wrbA"
                     /locus_tag="ACG0BW_004068"
     CDS             6183..6779
                     /gene="wrbA"
                     /locus_tag="ACG0BW_004068"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFK7260969.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6800..7027
                     /gene="yccJ"
                     /locus_tag="ACG0BW_004069"
     CDS             6800..7027
                     /gene="yccJ"
                     /locus_tag="ACG0BW_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFK7260970.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7065..8306)
                     /gene="agp"
                     /locus_tag="ACG0BW_004070"
     CDS             complement(7065..8306)
                     /gene="agp"
                     /locus_tag="ACG0BW_004070"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFK7260971.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(8599..>9515)
                     /locus_tag="ACG0BW_004071"
     CDS             complement(8599..>9515)
                     /locus_tag="ACG0BW_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260972.1"
                     /translation="DIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYC
                     LEDENISVQPEQINTPGISDLDTGIDLSGISFIQSEINKTYELKYAPVDGDGYCLLRA
                     ILVLKQHDYSWALGSHKTQKEVYEEFIKIVDKPTIEALVDAAFCDLREDVKTLFGVDL
                     QSDNKIQGVGSLMSWSFLFFKKEFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSD
                     RIKAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYL
                     PPELFAQAYKLNNKEMNAQLDYLNRYAI"
BASE COUNT         2219 a         2442 c         2463 g         2391 t
ORIGIN      
        1 ttttaaaaac tggcatccgc tttgcaaaca aaccattacg cggctgaaaa ggatatgcag
       61 gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt
      121 ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac
      181 atgccgacct ttgaactgaa taaagccatt gtgcaaaaag cggagcacta ccatttcgat
      241 ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac
      301 aaccttgagt cgttcacctt gatggcgggg ctggcagccg tgacctcgcg cattcagata
      361 tacgcgacag ctgccacctt aacgttacct ccggcaatcg tcgcccgtat ggccgcaacc
      421 attgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcaaaagcct
      481 gagtatgagc agatgggtat ctggcctggc gatgactatt tctcccgtcg ttacgactat
      541 ctcaccgaat atgttcaggt gctgcgcgac ctgtggggca cgggcaaaag cgattttaaa
      601 ggcgattttt tcaccatgaa tgattgtcgc gtcagtccgc aaccgagcgt acccatgaaa
      661 gtgatctgcg ccgggcaaag cgacgctggc atggcgttct ccgctcagta tgccgatttc
      721 aacttctgtt tcggcaaagg cgtaaataca cccacggctt tcgccccgac cgctgcgcgg
      781 atgaaacagg ccgcagagca aaccgggcgc gacgttggct cttatgtgtt gtttatggtg
      841 attgccgatg aaaccgacga tgccgctcgc gccaaatggg aacactacaa agcgggcgcg
      901 gatgaagagg cgttaagctg gctaaccgaa caaagtcaga aagatacccg ctccggtact
      961 gacaccaacg tccgtcagat ggccgatccc acttcggcgg taaacatcaa catggggacg
     1021 ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag cgtgcctggt
     1081 gccgaaggcg tgctgttgac cttcgatgat tttctgtcgg gaatcgaaac cttcggcgag
     1141 cgcattcaac cactgatgca gtgccgcgcc catctccctg cgctgactca ggaggtggca
     1201 tgatgacaat cttaaccgct cgaccggaag ccattacctt cgatccgcag caaagtgcgc
     1261 tgatcgtggt ggatatgcaa aacgcttatg ccacgccagg cggctactta gatctcgccg
     1321 ggtttgatgt ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc gtgaccgcgg
     1381 cacgagcagc agggatgctg atcatctggt ttcaaaatgg ctgggatgaa cagtatgtcg
     1441 aggctggcgg ccccggctca ccgaattttc ataaatcgaa cgccctgaaa accatgcgta
     1501 agcagccgca gctgcagggg aaattgctgg cgaaaggctc ctgggattat caactggtgg
     1561 atgaactggt gccgcagcct ggcgatattg tgctgccgaa gccgcgctac agcggtttct
     1621 tcaatacgcc gctggacagc attttgcgca gccgtggaat acgccatctg gttttcaccg
     1681 gtatcgctac caacgtctgc gtcgaatcga cgctacgcga tggctttttt ctggagtatt
     1741 tcggcgtggt gctggaagac gcaacacacc aggcggggcc ggaatttgca cagaaagccg
     1801 cgttattcaa tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg ttctgcgacg
     1861 cgctttctcc cacgtccttt gctcgtatcg cttaaggagt ttaacgatgc caaaatccgt
     1921 aattattccc gctggcagca gcgcaccgct ggctcccttc gttcccggca cgctggctga
     1981 tggcgtggtg tatgtctccg gtacgctggc ttttgatcaa cataataacg tgctgtttgc
     2041 cgatgaccca aaggcgcaaa cccgccacgt tctggaaacc atccgcaagg tgatcgagac
     2101 ggcgggtggc acgatggcgg acgtgacctt caacagcatc tttattaccg actggaaaaa
     2161 ttacgccgcg attaacgaaa tctactccga gtttttcccg ggtgacaaac cggcgcgatt
     2221 ctgcattcag tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca
     2281 tatcgccaag tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatgcgc
     2341 ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag ttactggtta ccgcaactgg
     2401 cggtactgga gcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc
     2461 ccgacacgct ggcagaagat tacagtatcg cccagatggc agcggaactg catcaggcgc
     2521 tggtagccgc agggattaag cgttacgcgg tggtcggcca tgcgctcggt gcgctggtgg
     2581 gaatgcagct ggcgctggac tatcccgcgt cggtaactat gctggtcagc gttaacggct
     2641 ggctacgaat aaacgcccat acgcgccgct gttttcaggt tcgcgaacag ttactacata
     2701 gcggcggcgc gcaggcatgg gtggaagctc agccattgtt cctctatccc gccgactgga
     2761 tggcggcccg cgcacctcgc ctggaagctg aagacgcgct ggcactggcg cattttcagg
     2821 gcaaaaataa tttactgcgt cgacttaacg ccctcaaacg cgctgacttt agtcaccatg
     2881 cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccat
     2941 cagcatgttc cagtgaactt catgccgccc tgcccgatag ccagaaaatg gtgatgcgct
     3001 atggcggaca cgcctgcaac gtgacccatc ccgaaacgtt taatgctctg ttactcaacg
     3061 ggcttgccag cctgttacat caccgtgaag ccgcctgtaa ggaattgcta tgaacgaagc
     3121 cgttagccct ggtgcgctta gcaccctatt caccgatgcc cgcactcaca acggctggcg
     3181 ggagacaccc gtcagcgatg agacgttacg ggagatttat gccctgatga aatgggggcc
     3241 gacatcagct aactgttctc cggcacggat cgtgtttatc cgcactgcag aaggaaaaga
     3301 acgtctgcgc ccggcgcttt ccagcggcaa tctgcaaaaa accctgaccg cgcccgtcac
     3361 cgcgatcgtc gcctgggaca gtgaatttta tgaacggtta ccacagctgt ttcctcacgg
     3421 cgatgcccgc agttggttta cctccagccc acaacttgcc gaagaaacag cgtttcgcaa
     3481 cagttccatg caggcggcct atctgatcgt cgcctgccgg gcgctggggc tggataccgg
     3541 cccgatgtcc ggctttgacc gtcaacacgt ggacgacgcc ttttttacgg gcagcacgct
     3601 gaagagcaat ctgctgatta atatcggcta tggcgatagc agcaagcttt atgcgcgcct
     3661 gccacgtctg tcctttgaag aagcctgcgg gctgttgtaa ggagcaaact atgaacattg
     3721 tcgatcaaca aacttttcgc gatgcgatgt cctgcatggg cgcggcggtc aatattatca
     3781 ccacggacgg tccagccggg cgcgccgggt tcaccgccag cgccgtctgc agcgtgaccg
     3841 atacgccgcc cacattactg gtatgcctga atcgtggggc gtccgtctgg ccggtattca
     3901 atgaaaatcg aacgctgtgt gtaaatacac ttagcgccgg acaggagccg ctttcaaacc
     3961 tttttggcgg caaaacgccc atggaacacc gctttgccgc cgcccgctgg cagactggtg
     4021 tgaccggatg cccgcaactg gaagaggcac tggtttcgtt tgactgccgt atcagccagg
     4081 tggtgagcgt cggcacccac gacattctgt tttgcgccat cgaagcgatt catcgtcacg
     4141 ccacacccta cgggctggtg tggttcgatc gcagttatca cgcgctgatg cgccctgctt
     4201 gttaacctcc ttaaggagac agctgatggc aatgttcggt cttcctcact ggcagttaaa
     4261 atcgacctct acagagagcg gcgtggtcgc gccggatgaa cgattgccgt ttgctcagac
     4321 ggcaataatg ggcgttcaac acgcggtggc gatgtttggc gcgacggtgt taatgccgat
     4381 tctgatgggg ctggatccca atctttccat tttaatgtca gggatcggca cgttactgtt
     4441 tttctttatt accggcgggc gcgtgcccag ttatctcggc tccagcgcgg cttttgtcgg
     4501 cgtggtgatc gccgcaacgg gctttaacgg tcagggcatt aacccgaata tcagcattgc
     4561 gctgggcggg attatcgcct gtgggctggt ttataccgtc attggcctgg tggtgatgaa
     4621 aatcggtacg cgctggattg aacggctaat gccgccggtg gtgacgggtg cggtggtgat
     4681 ggcgattggc ctgaacttag cgcctatcgc agtgaaaagt gtatcggctt cggcctttga
     4741 tagctggatg gcggtaatga cggtgctgtg tattgggctg gtggcggtgt ttacccgtgg
     4801 catgatccag cgattattga ttctcgtagg attaattgtc gcctgtctgc tgtatggcgt
     4861 gatgaccaac gttttagggc tgggaaaagc cgtcgatttt actctcgtca gccatgctgc
     4921 ctggttcggt ctgccgcatt tttccactcc tgcttttaat ggtcaggcaa tgatgctgat
     4981 tgcgcccgtg gcggtgattc tggtggcgga aaacttaggt catctcaagg cggtcgccgg
     5041 gatgaccgga cgcaatatgg acccgtatat ggggcgagca ttcgtaggcg atggtctggc
     5101 aacgatgctt tccggctctg tcggcggtag cggcgtgacc acctatgctg aaaatatcgg
     5161 tgtgatggcc gtgaccaaag tctactcgac gctggtgttt gttgctgctg ccgtcatcgc
     5221 catgctgttg ggcttttcgc cgaaatttgg cgcgctgatc cataccattc ctgcaccggt
     5281 gattggtggt gcatcaattg ttgtgttcgg gctgattgcc gtcgcaggcg caaggatctg
     5341 ggtacaaaac cgtgtcgatt taagccagaa cggtaatttg attatggtcg cagtaacctt
     5401 agtgctgggc gcgggtgatt ttgctctcac gctgggcggt tttacgttgg gagggatcgg
     5461 tacagcaacc tttggcgcga ttttactcaa tgcgttgtta agcagaaggt tggttgacgt
     5521 tccaccgcca gaagttgttc atcagtagcc ataaatagaa aaccgccggg gtaaccggcg
     5581 gtgatgactt tttgacgagc ttcagacaaa aatgcatgcg tcattgatta gcccggctag
     5641 ttgtcgcttt tgccgtgact gctcttgcca ccttttttac ctgcttcaga tgcgcgctgc
     5701 gggtcatttt tgaaattacc cccgctgtgc tgtccacctt ttttacctgc ttctgatgct
     5761 ctttcgcggt cttctgcaaa attgccggaa ccgcctcgat ggtttgccat tactgacctc
     5821 cgtctgggta tagacatcat attgcataga attaacgcgg cgtatggcgg ctgccatatt
     5881 ttcgctacgt gggactaagc ttagtgaact gacgcgggca gacccgcgtt tagtaatatc
     5941 ctgcaacagc ttcttagcga gaatagatat catctccaat aattatccat aagccgctct
     6001 tatgattaaa aaaacgcgat gagataaaaa tgtgtcattt tgcgacaaaa ttacgtgctt
     6061 gtgaaagttg ttataaatca aataagtggt tgtgaaattt gcactctgaa aaggacgtct
     6121 tatctttaaa taagtggtag cgaatcgcta cggaatagag ataacacgag gagtggttag
     6181 aaatggctaa agttctggtg ctttattatt ccatgtacgg acatatcgaa acgatggcac
     6241 gcgcagtcgc tgagggtgca agcaaagtgg atggcgcaga agttgtcgtt aagcgtgtac
     6301 cggaaaccat gccgccgcaa ttatttgaaa aagcaggtgg taaaacgcaa actgcaccgg
     6361 ttgcaacccc gcaagaactg gccgattacg acgccattat ttttggtaca cctacccgct
     6421 ttggcaacat gtccggtcaa atgcgtacct tcctcgacca gacgggcggc ctgtgggctt
     6481 ccggcgcact atacggaaaa ctggcgagcg tctttagttc caccggtact ggcggcggtc
     6541 aggaacaaac catcacatcc acctggacga cccttgcgca tcacggcatg gtaattgtcc
     6601 ccattggcta cgcagcgcag gaattatttg acgtttcaca ggttcgcggc ggtacgccgt
     6661 acggcgcaac caccatcgca ggcggtgacg gttcacgcca gccaagccag gaagaactgt
     6721 ctattgctcg ttatcaaggg gaatatgtcg caggtctggc agttaaactt aacggctaat
     6781 cttcaacagg aggatacgca tgccaactca agaagcgaaa gctcatcacg tcggtgaatg
     6841 ggcatctctg cgcaatacgt cgccggaaat agccgaggcc attttcgaag tcgccgggta
     6901 cgacgaaaaa atggcggaaa agatttggga agaaggtagc gatgaagtgt tagtcaaagc
     6961 ctttgctaag accgataaag attcgctttt ttggggcgaa cagaccatcg aacgtaaaaa
     7021 cgtttaatac gcgactcccc cgcttcacgc gggggagttt tctgttattt caccgcttca
     7081 ttcaatacgc tatcaaactt atccattggg cagaaaccgt tggcatctat cggacaaccg
     7141 cttaattcca gcgtcacgcg ctgcgcaggt gcctgaaggg ttaacgcgtc agcattacgt
     7201 aactgttctg cactctggta cacatactcg attttcatca ggtcgcgatt ggctttgctg
     7261 tcatgccaac gctggaaaac gattttgccg ccaatcggcg tgcgttcgtt ctggtcatgc
     7321 aactgatacg gtttgaaatc cagcgccgtt aacagagagg caatgttgga gtcgtgccca
     7381 actaacaccg tgattttcgg tgcactggtg cgatcggtga ccagggcttt gtcgatataa
     7441 ctgaccagcg gtttcgcaac attgcgcgcc acttccggtg aggtaaacag gctgtcctgg
     7501 taaccgtttt taagcttcga caacaccttc cactgctggt cagatttgat ttctccccag
     7561 gccacctgat ccatcggaaa accttcgtag tattgcaaag taaacgcatc taccagcgag
     7621 ttgccgactt tcagcggccc ggaaacacct ggttcttgtt gatacttcgc gctaaaggta
     7681 tttttgccat ccaccagcga acattgttgt ttctctttac aggcagggga atctttatag
     7741 ttaacgattt tttccagtag ctggtagctg tcggtaagct ggagtttgct gagctctttc
     7801 tccattgccg ccaccgcctg ttcactgaat gcggcggaat catcggtgat cactgggtta
     7861 aaggttgggt ccatagtgcc cattttttcc tggtgatgca caggaatatc acacccgggg
     7921 aatgcgccgg taataaagaa ctgtgcagtg gcgacggtgc gttgcagact attggcatag
     7981 gcataaacgg tgtccggcgg cgggcattcc cccgatttca ccatcccctg ttctgccagc
     8041 cattcacgca tgtaatggcc catatacact tcgagcacgc cacctttggt ggtgagttgc
     8101 ccaccgggga cgtcccattc tggccattta ttcggcgtcg actgctccag cacactgcca
     8161 ttgttcgcca gcggcgcacg taagttatgg cggctcatca tgagcacttg ctgtagctga
     8221 tagccttccg gtacggtttg tgcctgagca tttgaagcga gtaaaactat ccctgccaca
     8281 gttgcggcga ttagcgtttt gttcattcct ggcacctctt ttgttatcaa tcactaaaga
     8341 gtgtgacaga aatatgactt ttttccggga acaggttcgc aaaatggctt gttgaaaacc
     8401 tgtttttcta tatacatctt caaaaacagg caaggtttga cgcaaactat gcctgattag
     8461 cccctgcctc gcgtacgccg ctgataattg cacccgtggc taaacagaat atctgcggcg
     8521 ttaccaatac agagacaata tttaacaata aaaaagtgtg agactaataa caatggtttt
     8581 tagcatatgc ctttgccatt aaattgcata acgatttaaa tagtcaagtt gcgcattcat
     8641 ttccttattg tttaacttgt aagcctgagc aaaaagttca ggagggaggt aaacgtcata
     8701 atgattgccg gtatttatta aataaccata ctcaagatct tcatctgttg aaaaggattc
     8761 atttcccaag gagattgatg ccacattact attaacaata aataatgagc aaatgctatc
     8821 tgaaagaaca gcaagaaaat tcttcaccgc tttaatccta tccgacgtgt cggtatctaa
     8881 agcaagcaaa ttcttgttat cattaaatat aaactcgggt aaatgcagga tacatttttc
     8941 gttattcaag caactatcaa tgaattcttt tttaaaaaac agaaagctcc atgacataag
     9001 actacccaca ccctgaattt tgttgtcaga ttgcagatca acgccaaata atgtcttaac
     9061 atcttccctg agatcacaaa atgcagcatc aacaagagcc tcgattgttg gtttatcaac
     9121 aattttaata aactcttcgt atacttcctt ttgcgtctta tgactgccca gcgcccatga
     9181 ataatcatgt tgttttaaaa ccagtatggc tcttaacaga caatatccat cgccatctac
     9241 tggagcgtat tttaattcat aagttttatt aatttcactc tgaataaaag atataccaga
     9301 caaatcaata ccagtatcta aatcagatat tcccggcgta tttatctgtt ctggctggac
     9361 acttatattt tcatcttcta aacaatactc agaaaaaaca tcatcacacg cattaatgtt
     9421 ttcaatatat tccataaagc cataccaata ataaccttta aaatctgtga tagactctga
     9481 ccctctgccc ggtaaggaca ctctaacaat atcaa
//
LOCUS       DAYPHN010000173         9509 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000173 DAYPHN010000000
VERSION     DAYPHN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.173"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            301..882
                     /gene="mdaB"
                     /locus_tag="ACG0BW_004072"
     CDS             301..882
                     /gene="mdaB"
                     /locus_tag="ACG0BW_004072"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFK7260973.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            913..1227
                     /locus_tag="ACG0BW_004073"
     CDS             913..1227
                     /locus_tag="ACG0BW_004073"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFK7260974.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1275..3167)
                     /gene="parE"
                     /locus_tag="ACG0BW_004074"
     CDS             complement(1275..3167)
                     /gene="parE"
                     /locus_tag="ACG0BW_004074"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFK7260975.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3196..3777)
                     /gene="yqiA"
                     /locus_tag="ACG0BW_004075"
     CDS             complement(3196..3777)
                     /gene="yqiA"
                     /locus_tag="ACG0BW_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFK7260976.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3777..4604)
                     /gene="cpdA"
                     /locus_tag="ACG0BW_004076"
     CDS             complement(3777..4604)
                     /gene="cpdA"
                     /locus_tag="ACG0BW_004076"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFK7260977.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4629..5051)
                     /locus_tag="ACG0BW_004077"
     CDS             complement(4629..5051)
                     /locus_tag="ACG0BW_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFK7260978.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5052..5681)
                     /gene="nudF"
                     /locus_tag="ACG0BW_004078"
     CDS             complement(5052..5681)
                     /gene="nudF"
                     /locus_tag="ACG0BW_004078"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFK7260979.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5886..7367
                     /gene="tolC"
                     /locus_tag="ACG0BW_004079"
     CDS             5886..7367
                     /gene="tolC"
                     /locus_tag="ACG0BW_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFK7260980.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7515..8186
                     /locus_tag="ACG0BW_004080"
     CDS             7515..8186
                     /locus_tag="ACG0BW_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFK7260981.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8192..9352
                     /locus_tag="ACG0BW_004081"
     CDS             8192..9352
                     /locus_tag="ACG0BW_004081"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFK7260982.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2394 a         2547 c         2328 g         2240 t
ORIGIN      
        1 tataattatc aacatgacac gttgatactt tcatctttta cttattcaaa gccatatttt
       61 caatccccaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta agaattgtaa
      121 gaattatgag gaatggctat cagtattgtc attttcagaa aatatttatc ctgcatcggt
      181 gagtcagagt aagatcagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat
      241 ttcaccgtac cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa
      301 atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg
      361 aacgacaccc tgaccgaagt cgcggatggc acactgcgcg accttgggca tgatgtccgc
      421 atcgttcgcg ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct
      481 gatgtggtga tctggcagat gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa
      541 tacattgatg atgtattcac cgaaggtcac gggacgctgt atgccagcga tggtcgtacc
      601 cgcaaagatc cgtcgaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg
      661 ctttctctga cctggaacgc accaatggaa gccttcaccg aaaaagatca gttcttccac
      721 ggcgttggcg ttgacggtgt gtatctgccg ttccataaag caaaccaatt cctcggtatg
      781 gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat
      841 actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct caggctttag
      901 aaggagttaa ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac
      961 cgtcaggcgg tattggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt
     1021 tgccacggct atgcgccaat ggtggattgc gcagctggcg tgagtttcca gtctatggca
     1081 ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg
     1141 caaaccccgc acatgaaggc gtatagcgaa gccgtaaaag gtgacgtgct ggagatgaat
     1201 atccgtattc tgcagccagg gatttaatcc tgccttgttt gcccggccat cctgaccggg
     1261 caatgttctt tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg caaccagttg
     1321 cggcgatctt ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc agtacgctga
     1381 tcgtcttcat catcgatagt caactgcacc agacggcgag tgttcggatc aagcgtggtt
     1441 tcgcgcaatt gcatcgggtt catttccccc agacctttaa aacgctggac gttcggcttg
     1501 cctttcttgc gttttaattg ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa
     1561 taaacctctt tcccgagatc aatacggtag agcggtggca gtgcgacgta aacgtgaccg
     1621 tgtttcacca acgcgcggaa atgttttacg aacaaagcgc agagcagcgt ggcaatgtgc
     1681 agaccatcag agtccgcatc cgcgaggata cagattttgc cataacgaag ctggctcaga
     1741 tcgtcgctgt caggatcgat accgatcgct accgaaatat cgtgcacttc ctgcgaagcc
     1801 agcacttcgt cggaagagac ttcccaggtg ttaaggatct tacctttcag tggcatgatc
     1861 gcctgatatt cgcgatcgcg cgcctgcttg gcagatccgc ctgcggagtc accttccaca
     1921 aggaacagct cggtacggtt aaggtcctgc gcggtacaat cagccagttt gccaggcaac
     1981 gccgggccgc tggtcagctt tttacgcacc acttttttgg ccgcacgcat acggcgctgg
     2041 gcgctggaaa tcgccatctc cgccagcagt tcagccgcct gaacgttctg gttcagccac
     2101 aggataaagg catctttcac cacgccagaa acgaatgccg cgcattgacg cgaagagaga
     2161 cgctctttcg tctgcccggc aaactgcgga tcctgcattt ttactgacag cacataggcg
     2221 cagcgatccc agatatcttc cgccgacagc tttacaccgc gcggcagaat attgcggtat
     2281 tcacagaact cacgcatcgc gtccaacagg ccctgacgca gaccattaac atgggtaccg
     2341 ccctgcatcg ttgggataag gttgacgtag ctttcggtca gcagttcacc gccttccggc
     2401 agccacagta gcgcccagtc cacagcttca gtatcaccag cgaaattacc gataaacggt
     2461 ttttccggca gcgtcggcag accatttacc gcttccgcca ggtaatcatt cagaccgtcc
     2521 tgatagcacc agcgttgttc ggtattgttg atctcatctt taaaagtgat ctcaacgcca
     2581 gggcacaata ccgctttggc tttcagcaca tgcgtcaggc gtgaaacaga aaatcgcggg
     2641 ctgtcaaaga aggtttcatc cggccagaag tgcacactgg taccagtatt gcgtttaccg
     2701 caagtgccga caacctgtaa atcctgcacc ttttcgccat tttcaaaggc gatgttataa
     2761 acctgaccat cgcggcgtac gttaacttct acgcgcttcg acagggcgtt aactaccgaa
     2821 atccccacgc catgcaggcc gccagagaac tggtagtttt tgttagagaa tttaccgcct
     2881 gcatgcagac ggcaaagaat cagttcaacc gccggtacac cctcttccgg gtgaatatcc
     2941 accggcatcc cgcgcccatc gtcaatcact tctaacgact ggtcagcatg taagattaca
     3001 tccacgcgtt ttgcgtgacc cgccagtgct tcatcgacac tgttatcaat aacttcttgc
     3061 cccaaatggt tagggcgagt ggtatcggta tacatccccg gacggcggcg aaccggctca
     3121 agcccggtga gtacctcaat ggcatcagcg ttataagttt gcgtcatggt ttaagttagc
     3181 aattcgagtt gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg ggttgaaata
     3241 atcttcgaag cccgtgaatg cgtggttgcc gccttctatg acagtctggc ggcaggaagc
     3301 gtagtacgcc accgcctggc ggtaatccag cacttcatct cccgtctgtt gcagcagcca
     3361 gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact ttgagatcgt aaatatggcg
     3421 tgactctagc acatattgct gcccggtgta ggggttctcg ttctgaccga gatagtccgt
     3481 cagcagttca aacgggcgta ccgccgggtt taccaccact gcgggcagca taaaacattg
     3541 cgacaaccag gtggcgtaat atccccccag tgacgaaccg acaataccca gcgaatcgcc
     3601 gccatgttcc aggacaatgg attccagcag ttctgccgca tcggaaggat acggcggcaa
     3661 ctgcggaatg atcatctcta cgtcagggtg atgttccgcc aaccagtttt ttaacaagct
     3721 cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg tagacatcag
     3781 tagccttctg aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg
     3841 gtcagcgtgc catcagcatg taactcgaga gtacgccagc cgggcgcgat ggtgtccagc
     3901 gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg acggcgttgc cagcaggcgg
     3961 cgaccgttcc agtcgagatc cagctcctga tgaatatgac cgcacagcaa gtatttgacg
     4021 tgcggaaact tcgccagcac ggtatccagt tcgcccgcgt tacgcaggct gtgttgatca
     4081 agccaactac aacccgcagg tagcggatga tgatgtagca gcagcaacgt atggcgttct
     4141 ggcgcatcgg ccagtttacg ttccagccac tcaagctgaa actcgctcag ctcaccgtgc
     4201 ggcacgccaa acacctggct atccagcaac aggatttgcc attgctcacc aataaacacc
     4261 cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg
     4321 tggttgcccg gcagccagac gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc
     4381 tgataggccg cagaggattg atcctgcgct aaatcacctg tcgcgacaat caggtcgaat
     4441 tcgtgctggt gtggccgaat cgcctccagc accgcctggt agctttccca ggtgtttacc
     4501 cccaacaggg cttcgtgctt ttgcgcaaac aggtgagtgt cggtaatttg taaaatcctg
     4561 actctggcct caccagccag aggaagggtt aacaggcttt ccaaatggtg tccttaggtt
     4621 tcacgacgct aataaaccgg aatcgccatc gctccatgtg ctaaacagta tcgcaaccag
     4681 tccgctaaaa actgattaat ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga
     4741 taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc agccaccatc
     4801 gcgtcatgat acagacgcac cgtcattgac ggaaggctcc agtaactgat cgcgggcgca
     4861 gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat
     4921 tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa
     4981 cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg
     5041 taacgcttca tttatgccca ctcatttttt aacgcttgat gatgcagctg cagccattgc
     5101 aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt ctacccactg gtatgcctgt
     5161 tcccggctta ccacatgaac gcgaatatct tcgttttcat cagccagacc gtgaataccg
     5221 cttgcggtcg tggcgtccac ttcgcccacc ataattgacg aacgctcact ggtgcccccc
     5281 gggcttgcca ggaaacttaa caccggtttg gtccgtttga ctatcagtcc cgcctcttca
     5341 atcgcttcgc gacgggcaac atcttccaca ctttcaccct cttcaatcat cccggcaacc
     5401 atctccagta gccagggggt ttcgctggtg tcgtacgcgg caatccgaat ctgctcaatc
     5461 agcacaactt catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca
     5521 aaaatttccc gccgtacctc atgactcatt tgcccgttga atagacgatg acgaaatcta
     5581 taaagatcta atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat ttctacatcg
     5641 tttttgccaa atgtaacggg caggttgtct ggcttaagca ttgttaatgt cctggcacta
     5701 atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag tcatgtgtta
     5761 aattgaggca cattaacgcc ctatggcacg taacgccaac cttttgcggt agcggcttct
     5821 gctagaatcc gcaataattt tacagtttga tcgcgctaaa tactgcttca ccacaaggaa
     5881 tgcaaatgaa gaaattgctc cccattctta tcggcctgag cctttctggg ttcagttcgt
     5941 tgagccaggc cgagaacctg atgcaagttt atcagcaagc acgccttagt aacccggaat
     6001 tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc
     6061 cattactgcc acagctaggt ttaggtgcag attacaccta tagcaacggc taccgcgacg
     6121 cgaacggcat caactctaac gcgaccagtg cgtccttgca gttaactcaa tccatttttg
     6181 atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc agctgggatt caggacgtca
     6241 cgtatcagac cgatcaacaa accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt
     6301 tgaatgctat tgacgttctt tcctatacac aggcgcaaaa agaagcgatc taccgtcaat
     6361 tagatcaaac cacccaacgt tttaacgtgg gcctggtagc gatcactgac gtgcagaacg
     6421 cccgcgcaca gtacgatacc gtgctggcga acgaagtgac cgcacgtaat aaccttgata
     6481 acgcggtaga gcagctgcgc cagatcaccg gtaactacta tccggaactg gcggcgctga
     6541 atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa cacgctgctg aaagaagccg
     6601 aaaaacgcaa cctgtcgttg ttacaggcgc gcttgagcca ggacctggca cgcgagcaaa
     6661 ttcgccaggc gcaggatggt cacttaccga ctctggattt aacggcttct accgggattt
     6721 ctgacacctc atatagcggt tcgaaaaccc gaggtgccgc tggtacccag tatgacgata
     6781 gcaatatggg ccagaacaaa gttggcctga gcttctcgct gccgatttat cagggcggaa
     6841 tggttaactc gcaggtgaaa caggcccagt acaactttgt tggtgccagc gagcaactgg
     6901 aaagcgcgca tcgtagcgtc gtacaaaccg tacgttcctc cttcaacaac attaatgctt
     6961 ctatcagtag cattaacgcc tacaaacaag ccgtagtttc cgctcaaagc tcattagacg
     7021 cgatggaggc gggctactcg gtcggtacgc gtaccattgt tgatgtgttg gatgcgacca
     7081 ccacgttgta caacgccaag caagagctgg cgaatgcgcg ttataactac ctgattaatc
     7141 agctgaatat taagtcagct ctgggtacgt tgaacgagca ggatctgctg gcactgaaca
     7201 atgcgctgag caaaccggtt tccactaatc cggaaaacgt tgccccgcaa acgccggaac
     7261 agaatgctat tgctgatggt tatgcgcctg atagcccggc acccgtcgtt cagcaaacat
     7321 ccgcacgcac taccaccagt aacggtcata accctttccg taactgatga cgacgacggg
     7381 gcttcggccc cgtctaaacg taaggcaacg taaagatacg ggttatctgc cgcattcttc
     7441 ccccttctcg cttcaatttc gaccagccat cctctattct gatgggtatt taccactggt
     7501 cccggaagac aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact
     7561 ggagcgcacg ccatctgaca ccagtcgctc tcgcggttgc cactgttttt atgctggctg
     7621 gctgtgaaaa gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac tgttcagctg
     7681 caaacccagg caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg
     7741 aacgtactgc gccgaaatac gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc
     7801 agtgccagca ggcaccagcc caggctggta tggcaccaga aaaccaggcg caggcccagc
     7861 aatccagcgg aagtttctgg atgccgctga tggccggtta catgatgggg cgtctgatgg
     7921 gcggcggcgc gggatttgca cagcagccgc tgttctcctc gaaaaaccca gccagcccgg
     7981 cttacggtaa atataccgac gcgacgggta aaaactatgg cgcagcgcag ccaggccgca
     8041 ccatgaccgt accgaagacg gcaatggcac caaaaccggc gaccaccacc accgttactc
     8101 gtggcggttt tggtgaatct gttgccaaac aaagcactat gcagcgtagc gcaaccggca
     8161 cctcttctcg ttcaatgggt ggctgatacc gatggaaaga gtcagtatta ccgagcgccc
     8221 ggactggcgc gaaaaagccc acgaatacgg tttcaatttt cacaccatgt acggcgaacc
     8281 gtactggtgt gaagatgctt actacaagtt gaccctcgcc caggttgaaa agctggaaga
     8341 agtcaccgcc gaactgcacc agatgtgcct gaaagtggtt gaaaaagtga tcgccagcga
     8401 tgagctgatg accaaattcc gcattccaaa acacacctgg agttttgtgc gccagtcatg
     8461 gctgactcac cagccatcgc tttattcgcg tcttgatctg gcgtgggatg gtactggcga
     8521 acctaaactt ctggaaaata acgccgatac gccaacgtca ctgtatgaag ccgcattctt
     8581 tcagtggatc tggctggaag atcagcttaa cgccggtaac ctgccggaag gcagcgacca
     8641 gtttaacagt ctgcaagaaa agctaatcga tcgctttgtc gagttgcgtg aacagtatgg
     8701 cttccagttg ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg gaaccattca
     8761 gtatttgcag gactgcgcga cagaagctga aatcgctact gaattcctct acatcgacga
     8821 tatcggttta ggcgaaaaag gtcagttcac ggatttacaa gatcaggtga tttctaacct
     8881 gttcaagctg tatccgtggg aatttatgct gcgtgagatg ttctccacca agctggagga
     8941 tgcaggcgta cgctggctgg aaccggcgtg gaagagcatt atctccaaca aagcgcttct
     9001 accgctgttg tgggagatgt tcccgaatca cccgaacctg ctgcccgctt attttgcgga
     9061 agatgatcat ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc
     9121 aaacgtgtcg atcattgaga acggcaaaac cattgaagca gcggaaggtc cgtatggcga
     9181 agaagggatg attgttcagc aattccaccc gttaccgaaa ttcggcgaca gctatatgct
     9241 gattggtagc tggctggtta acgatcaacc tgccggaatt ggcattcgtg aagaccgtgc
     9301 attgatcacc caggatatgt ctcggtttta tccgcatatt tttgttgagt aatactgttc
     9361 aatattgccc cgtttaccgg ggcaatttat tatacttcgc aaatcatcac ccccatctat
     9421 atataatcaa tcacatccat cacttttatt taacattaat tcctaattta atattaaatc
     9481 gtcatatgta agcgatactg taattaaat
//
LOCUS       DAYPHN010000174         9031 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000174 DAYPHN010000000
VERSION     DAYPHN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.174"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..500
                     /locus_tag="ACG0BW_004082"
     CDS             <1..500
                     /locus_tag="ACG0BW_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFK7260983.1"
                     /translation="ECVMLKISLYVIILLFSFQISAAITSKESEVVSPLLMDVNSSLT
                     MENISELSTSSEPSQQGVFPVICTRLHPGSVMKRQLLTGWGPVFIIGDDPFSLRWMSE
                     HLEILKSLNALGLVVNVESVERMEVLQQRADGLLLLPVICDNFVQTLQLNAYPVLITE
                     MEISQ"
     gene            511..720
                     /locus_tag="ACG0BW_004083"
                     /pseudo
     CDS             511..720
                     /locus_tag="ACG0BW_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(772..1542)
                     /gene="yafV"
                     /locus_tag="ACG0BW_004084"
     CDS             complement(772..1542)
                     /gene="yafV"
                     /locus_tag="ACG0BW_004084"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFK7260984.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEDITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAALREYREKFPAWRDADEFRLW"
     gene            1723..2169
                     /gene="ivy"
                     /locus_tag="ACG0BW_004085"
     CDS             1723..2169
                     /gene="ivy"
                     /locus_tag="ACG0BW_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFK7260985.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2212..4656)
                     /gene="fadE"
                     /locus_tag="ACG0BW_004086"
     CDS             complement(2212..4656)
                     /gene="fadE"
                     /locus_tag="ACG0BW_004086"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFK7260986.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4896..5474
                     /gene="lpcA"
                     /locus_tag="ACG0BW_004087"
     CDS             4896..5474
                     /gene="lpcA"
                     /locus_tag="ACG0BW_004087"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFK7260987.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5680..6447
                     /gene="yafJ"
                     /locus_tag="ACG0BW_004088"
     CDS             5680..6447
                     /gene="yafJ"
                     /locus_tag="ACG0BW_004088"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFK7260988.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6418..7158)
                     /gene="dpaA"
                     /locus_tag="ACG0BW_004089"
     CDS             complement(6418..7158)
                     /gene="dpaA"
                     /locus_tag="ACG0BW_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFK7260989.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7459..8208
                     /gene="yafL"
                     /locus_tag="ACG0BW_004090"
     CDS             7459..8208
                     /gene="yafL"
                     /locus_tag="ACG0BW_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFK7260990.1"
                     /translation="MSSFFHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            8385..8882
                     /gene="rayT"
                     /locus_tag="ACG0BW_004091"
     CDS             8385..8882
                     /gene="rayT"
                     /locus_tag="ACG0BW_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFK7260991.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDFSAG
                     ERIIL"
BASE COUNT         2226 a         2342 c         2253 g         2210 t
ORIGIN      
        1 ttgaatgtgt tatgttaaaa ataagtttat acgtaataat attgttattt tcctttcaga
       61 ttagtgcagc cattactagc aaggaaagtg aggttgtttc accattatta atggatgtta
      121 attcttcact aacaatggaa aatatttctg aattatccac atcatcggaa ccatcgcaac
      181 agggggtatt tcctgtaata tgtactcgac ttcatcctgg ctccgtaatg aaacgccagt
      241 tattaaccgg ctgggggcct gtttttataa ttggtgatga tcctttttct ctgcgctgga
      301 tgagtgaaca tctggagatc cttaaatcgc taaatgccct tggtttggtt gttaatgttg
      361 aatcagtaga acgaatggag gtattacagc agcgggctga tgggttattg ttattgccag
      421 ttatctgcga taatttcgtg caaactctgc aattaaatgc ttatccggta ttaatcacag
      481 agatggaaat atcacaatga ttaataacat aattactgca aataatatcg taattttgaa
      541 aatggtaatt ctgttcacgg caccataatt agagttatgt cttgtatccg ttcagtataa
      601 tttcacgaat gctttattaa ctggatgcga gattgccatt catcaaatga taaagacgtc
      661 atcgcaattg atggaaaagc gcttccacac tcctatgata aaagtggtcg caggggagcg
      721 ggctttcgta gtcttagccc gactctcccc ggccctaaac acaacaccca ctcaccacaa
      781 cctaaactca tccgcatccc gccatgccgg aaacttttct ctatattccc gcaatgccgc
      841 catcgacagc tccgcatcaa tgcgcgttgc ctgatgcgcg tcggcggtgg cgataatctc
      901 gccctgtgga ttaatcaccc ggctgtcacc gcgataatgg cagccgttgc catcgctgcc
      961 gacgcgattg catcccgcca catacgcttg attctcaatc gcgcgggccg tcagcaatgc
     1021 ctgccagtgc agagagcgcg gagccggcca gttggcgacg tacagggcga ggtcgtaatc
     1081 gttgagattg cgcgaccaca caggaaaacg taagtcgtag cacaccagcg gcaaaatacg
     1141 ccagccgcgc cattccacaa tcactcgcgc attgcccgct ttataatgca gatgctcatc
     1201 tgccatgcgg aacagatgac gcttatcata aaaatgtacc gtgccgcctg gctcaaccag
     1261 caaaaagcgg ttaaccgaac cagactccgt ttgtaatgca acactgcctg caatcagcgc
     1321 attgcactgc tgcgctttgg ctgtcatcca gttcactacg tcatcttgtg ctagcgacga
     1381 agctgccgct tccatggcaa agccgctggt aaacatctcc ggtagaacga tcacatcgcg
     1441 tccggtaata tcttccagtt gacgatcaaa atgacgcagg ttggcaggac catccatcca
     1501 caccagtggt tgctgcaaaa gcgtaatctt caaaccaggc acggtgtaca actcctttat
     1561 gcgaagggtt ttataacttt aacaccttat caggcagttg ccttagcgca gaataaattg
     1621 ataacaaatg ctgatattgg aaatatctga tttgcaaatt atcgtgttat cgccaggctt
     1681 taggaggtta ataacatggg caggataagc tcgggaggaa tgatgtttaa ggcaataacg
     1741 acagtcgccg ctctggtcat cgccaccagt gcaatggcgc aggatgattt aaccattagc
     1801 agccttgcaa agggcgaaac caccaaagct gcgtttaatc agatggtgca agggcataag
     1861 ctgcctgcat gggtgatgaa aggcggtact tatactcccg cacaaaccgt aacgttggga
     1921 gatgagacgt atcaggtgat gagcgcgtgc aaaccgcatg actgtggctc gcaacgtatc
     1981 gctgtgatgt ggtccgagaa atctaatcag atgacggggc tgttctcgac tattgatgag
     2041 aaaacgtcgc aagagaaact cacctggttg aatgtgaacg atgcgctttc gattgatggt
     2101 aaaacggtgc tgttcgcggc gttgaccggc agcctggaaa accatccgga tggttttaat
     2161 tttaaataat tagcggataa agaaacggag cctttcggct ccgttattca attacgcggc
     2221 ttcaactttt cgcactttct ccggcaactt taccggcttc gtcgcaagct cttccggatc
     2281 aaagtcatcg acattaatac tgcgcagacg gctttcttca gctttcacaa gaatagcggc
     2341 ttcatcttta tcaatcagcc ccttcgccag cgcgttatgc gccagttcat ccagacgggt
     2401 aaacggcagg tttttaccca gctctttaca gatccgctga tgaattgggt cggcggcaat
     2461 cacatccacc agcgcctctt ccagtaaacc aaccgggtta tgctcgctcg gggtcaggta
     2521 ctgaccgcga ccaatacgtg aacgggtggc gttcggcact tgtaaaatct tcgccacctt
     2581 atgatccagc ttgtcagacg gtgccagata atgacgtccg gtcgggaaga tcaccacatt
     2641 cagcagcccg gcaaccacgc ggttcgggaa gttttgcagt aaatcgtcca tcgcctgctc
     2701 tgcctgatac agcgcatcct gtacgcccca gtgcaccagc ggcaggtcgg cttcattacg
     2761 gccttcgtcg tcataacgct tcagaacggc agaggcgagg taaagctggc ttaaaatatc
     2821 ccccagacgt gccgagatac gctcgcgacg tttcaagctg ccgcccagca cagccatcga
     2881 gacatcagaa agcagggcga ggttggcgct caggcggttc aggtgctgat agtagcgttt
     2941 agtggcatcg ccggttggcg tgctgctggt taaaccgcgc gtcaggccca gccagaagct
     3001 gcgaactttg ttgctaccga cgtgaccgat atgtttgaac agcagtttat cgaacgcgtt
     3061 tacgtcattg ttcttcgccg cttccatctc ttccagcacg tacggatggc aacgaatcgc
     3121 cccctgaccg aagatcatca tgctacgggt cagaatgttg gccccttcaa cggtgatggc
     3181 aatcggtgcg ccctggtaag cacgggccag gaagttgctc tgcccgagca taatgccttt
     3241 gccaccagtg atatccatcg catcaataat cgactgctgc ccacggtggg tacagtgata
     3301 cttaacgata gccgacagca cggcaggttt ttcaccgagc ataatgccgt aggtaatcag
     3361 cgatgccgca gcatccatta cataagcgtt tccggcaata cgcgccagcg gctcttcaat
     3421 cccttccatc ttaccaatag agattttgaa ctgacggcgg atgtgcgcat acgcacccgt
     3481 tgccagcgct accgatttca cgccgccggt tgagttggaa ggcagggtga taccgcggcc
     3541 taccgagagg cactccacca gcatccgcca gccttgtccg gccattttcg gcccgccgat
     3601 aatgtaatcg atcggtacga agacatcttt accgcgcgtc ggtccgttct ggaacggcac
     3661 gttcagcggg aagtggcgac gaccaatttc cacgcccggc gtggtggtcg ggatcagcgc
     3721 acaggtaata cctaaatctt ctgcgccacc cagcagtttt tccgggtcgg atagtttaaa
     3781 cgccagacca agcacggtcg caatcggtgc gagcgtaatg taacgtttgt tccaggtcag
     3841 acgcatcccc agcacctgct ggccctgcca ttcccccatg cagacaattc cggtatccgg
     3901 aatcgcgccc gcatcggaac ccgcttccgg gctggtcagt gcaaagcaag ggatttcctg
     3961 accacgcgcc agacgcggca gatagtgatc tttctgctcg tcagtgccgt aatgttgcag
     4021 tagctcaccc gggcctaatg agtttggcac accgacggta atcgccagga tcccgctcac
     4081 gccggagagt ttttgcagca cgcgagactg ggcataagcc gagaactcca gcccgccgta
     4141 ctcttttttg atgatcatcg cgaagaaacg atgctcttta aggtacgccc acaactccgg
     4201 cggcagatcc gccagctcat gagtaatctg gaaatcattc gccatccggc aggcttcttc
     4261 taccgggccg tcgagaaacg cttgctcttc tgcggtcagg cgcggctgcg gatagttatg
     4321 cagctttttc cagtccggct tgccctggaa caagtcgccc tcccaccagg tggtgcccgc
     4381 atcaatcgct tctttctcag tgcgcgacat cggcggcatt accttacgga aaccgcgaaa
     4441 taccggcgcg gaaatcatcg acttacgcat aggcgcaaag ttaaatggca cgaggataat
     4501 ggccagaggc accagtaccc acgccgacca cagaccagca acgccgaggg cggctgtcca
     4561 ggcgagcaaa atcagactgc tgataaataa gctcacgcgg tgatagaaca acgcgccgag
     4621 caggacaacc gtagcgagaa tactcaaaat catcataacg aaaagcccct tacttgtagg
     4681 aggtctgacc acttgtgatg atatggttgt agtggatgta aaaacattta gcaatgtgtt
     4741 tacaatataa ttacaacaaa gctcacattg ttgctgtttt tatccgcact tcaggtcaaa
     4801 aagtcctggt catagcacct gcccgtactt ctcgcttttg gcggtatccg gtacactgca
     4861 ttttgtctat tacatttatg ctgaaggata tcctcatgta ccaggatctt attcgtaacg
     4921 aactgaacga agcggcggaa acgctggcta actttttaaa agatgacgcc aatattcacg
     4981 ccattcagcg cgcggcggtc ctgttagcag acagctttaa agctggtggc aaagtgcttt
     5041 cctgcggcaa cggcggctcc cattgcgacg ctatgcactt tgccgaagag ttgaccggtc
     5101 gctaccgtga aaaccgtccg ggctacccgg cgattgccat ttccgacgtt agtcatattt
     5161 cctgcgtcgg taacgatttt ggtttcaatg atattttctc ccgttacgtt gaagcggtag
     5221 gtcgcgaagg cgatgtactg ctggggatct ccacctcagg taactctgca aacgtgatca
     5281 aagcgatcgc ggcggcgcgt gagaagggga tgaaagtgat caccctgacc ggtaaagacg
     5341 gcggtaaaat ggctggcaca gcggatatcg aaattcgcgt accgcacttt ggttatgccg
     5401 accgcattca ggagatccac attaaagtga tccatatcct gatccagtta attgaaaaag
     5461 agatggttaa gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat ccggcatttg
     5521 tgcgctgatg cctgatgcga cgctgacgcg tcttatcatg cctacaaatc tgtacccgaa
     5581 ccgtaggccg aataatgcgt ttacgccgca tccgacctga aaatacttaa atcaatcttc
     5641 gccgggggcc atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt gctcgggatg
     5701 agcgccaacg tccctaccga tatctgcttt agtttcaccg ggcttgtaca gcgtggtggt
     5761 ggaaccgggc cacataaaga tggctggggc atcacctttt acgaaggtaa aggctgtcgc
     5821 acatttaaag atccacaacc cagctttaat tcccccatcg ccaaacttgt ccaggactac
     5881 ccgataaaat cctgttcggt tgtggctcat attcggcagg ccaatcgggg cgaggtggcg
     5941 ctggaaaata ctcacccgtt tacccgcgag ttatggggac gcaactggac ttatgcccat
     6001 aacgggcaac tgacgggcta caaatcactg gaaaccggca acttccgccc ggtcggtgaa
     6061 accgatagcg aaaaagcctt ttgctggctc ctgcataaat taacgcagcg ttacccgcgc
     6121 acaccgggca acatggcggc agtgtttaaa tatatcgcct cactggcgga tgaactgcgg
     6181 cagaagggcg ttttcaacat gctgctttca gacgggcgct atgtaatggc gtattgctcg
     6241 actaatttac actggatcac ccgccgcgcg ccgtttggcg tggcaacgtt gctggatcag
     6301 gatgtggaaa tcgacttcag ctcgcagacc acacctaatg atgtggtcac ggtgattgcg
     6361 acacagccgc tgacgggcaa tgaaacctgg caaaagatta tgccaggcga atggcgctta
     6421 ttttgcctcg gggagcgtgt agtttgatgc cagctgtggt tgcacaactt cgtggcttaa
     6481 cggcttgctg accacgtaac ggccattgac cacagaaacg gtcggtggct tacgggtttg
     6541 ctcaaagtag tcgtagcccg gtttcagttg ctcccagaag tccttaaagt tggaatactt
     6601 atggcgcttc atattagcgt cggtcatacg gaacgggtaa atactcactt gcacgctcgg
     6661 ctgaccaaac accagcgcac cagtaacgaa ctggaagatc tcatcaatac cctgattggt
     6721 catcgcgtag cagccgatgg aaacacaatc gccgtggatc atcaggtatt tcccttcata
     6781 accatgcgca cggtcatagg cattggggaa tccaatatta atcgctttgt agtaacggct
     6841 gtctggtttt aactgattac gctggacgct ataaaaccct tccgggcttt tgaaatcgcc
     6901 ctgacgctgt tttggtccta agccgccgga atatttacag attttatagc tgtcgagcag
     6961 ttgatattgc tcgcccattt tgacgtagag atcgagcgta cgttcttcct tgaagatctg
     7021 gatgtagaca ggggatccca tcaactgctg cttatactct ttgctcactg gcgtggtcga
     7081 gctactgctg cccagcaaac cggcaaacga aacgcacggg atcaacaaca tcgcaagaat
     7141 taatgcgatt ttacgcatac tgcttattcc ttgataaaac ggttacacac gccaggacgg
     7201 caaaatggat cccaaatcgg aatagtctgg atttggaagg ctcacattat caccaaaaga
     7261 gttttacgca agcctgtcgg cgcagggttt acaaatttag cacaggggca gttttcatta
     7321 gtccgaaaag cgcatgtatc atttatttcc taagattatg aaacctcaat agtaatatgc
     7381 aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc cccttcattc
     7441 agccggttta tgtccttcat gtcttcgttc tttcacggca gatttctgca tccaggcgtg
     7501 ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacatcaca tatctctttc
     7561 agctacgccg cccgccagcg gatgcaaaac cgtgcgcgtt tattaaaaca gtaccaaacc
     7621 catctgaaaa aacaggccag ctatattgtg gaaggcaatg ccgaaagcag aaaggcgctg
     7681 cgccagcaca accgcgaaca gataaaacag catccagaat ggtttcctgc gccgctcaag
     7741 gcgagtgaca gacgctggca ggcgctggcg gagaacaacc atttcttaag cagcgaccat
     7801 ctgcacaaca tcaccgaagt ggcgattcac cgcctggagc agcagcttgg taagccttac
     7861 gtctggggcg gcacgcggcc cgatcaaggc ttcgactgta gcgggttggt tttttatgcc
     7921 tacaacaaga tcctcgaggc gaagctcccg cgcacggcca atgagatgta tcactaccat
     7981 cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctgtt tttccatatt
     8041 cacagccgtg agattgccga tcatatgggc gtgtatctgg gagacggtca gtttatcgag
     8101 tcgccgcgca ccggggaaac cattcgggta agtcggttag ctgaaccttt ctggcaggat
     8161 cattttttag gcgcgcgcag gattttgacg gaagagacga ttttgtagga cggataaggc
     8221 gttcacgccg catccggcaa tagtgcaccg atgcctgatg cgacgctgcc gcgtcttatc
     8281 atgcctacga gtctgcgaat tttttgcgag ctgctttccc gatataacac aacttcattg
     8341 tcgacttttc cttttcttct tatcgttgag aaaaaggagt cgccatgtct gaatatcgtc
     8401 gttattacat caagggggga acatggtttt tcacggtaaa tttacggaat cgtcgaagcc
     8461 aacttttgac cacccagtac cagacactcc gtaacgccat tattaaagtt aagcaggcca
     8521 gaccttttga aattaacgcc tgggtagttt tgccagagca tatgcactgt atctggacat
     8581 tacccgaaag tgatgatgat ttttcctctc gctggcggga gattaaaaag cagttcaccc
     8641 atgcttgcgg attgaaaaat atctggcaac cgcgtttttg ggagcatgcc attcgcaata
     8701 caaaagatta tcgccatcat gttgattata tttatataaa tccagtgaaa catggctggg
     8761 taaagcaggt gagtgattgg ccattctcaa cgttccatcg cgatgtagcg cgagggttat
     8821 atcccattga ttgggcggga gacataacgg attttagtgc cggggagcgt attattttat
     8881 aattgtgcgc ggatgcctga tgcgacgcag gcgcgtctta tcatgcctac aaacttgtgc
     8941 cggatcggtg ggccggataa ggcgttcaca ccgcatccgg caattgtgca ccggtgcctg
     9001 atgcgacgct ggcgcgtctt atcatgccta c
//
LOCUS       DAYPHN010000175         8958 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000175 DAYPHN010000000
VERSION     DAYPHN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.175"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1198
                     /locus_tag="ACG0BW_004092"
     CDS             <1..1198
                     /locus_tag="ACG0BW_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFK7260992.1"
                     /translation="DYLVCKWCGKSFHYFKSHVANGNCEGIPESVKDADPDTVLKMYT
                     TQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVEYKTDLVAAHELLN
                     VTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKDIFMMLELGIPGYL
                     WGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARDGSTYFEPGLLALA
                     MKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRITPHERFAFIGTGNT
                     NGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNAGIVDEYAEKMVKF
                     AGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFINKLPSASAQAAREV
                     VQKIFG"
     gene            1379..4897
                     /gene="dnaE"
                     /locus_tag="ACG0BW_004093"
     CDS             1379..4897
                     /gene="dnaE"
                     /locus_tag="ACG0BW_004093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFK7260993.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESMRCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAINGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDSIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTLKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFIREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKT"
     gene            5023..5454
                     /locus_tag="ACG0BW_004094"
     CDS             5023..5454
                     /locus_tag="ACG0BW_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260994.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            5572..6600
                     /locus_tag="ACG0BW_004095"
     CDS             5572..6600
                     /locus_tag="ACG0BW_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFK7260995.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVATASATEDEGLDD
                     LLRELDSL"
     gene            6661..7605
                     /locus_tag="ACG0BW_004096"
     CDS             6661..7605
                     /locus_tag="ACG0BW_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFK7260996.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVNQ
                     IIAKDAEADDLAGLLVSRMAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDNSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVNKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            7605..7871
                     /locus_tag="ACG0BW_004097"
     CDS             7605..7871
                     /locus_tag="ACG0BW_004097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7260997.1"
                     /translation="MLKSIINGATTTPAQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            7874..8947
                     /locus_tag="ACG0BW_004098"
     CDS             7874..8947
                     /locus_tag="ACG0BW_004098"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFK7260998.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
BASE COUNT         2534 a         2117 c         2206 g         2101 t
ORIGIN      
        1 tgattacctt gtttgcaagt ggtgcggcaa atcatttcac tattttaagt cccatgtagc
       61 caatggtaat tgcgagggca ttcctgagtc agtaaaagat gccgatcctg acaccgtact
      121 gaaaatgtac acaacgcagt ttccagatga accaacgcta tcgaaaaagg cacttgatgc
      181 aattcaagct aaacgtgccg agcaaaaaag cgaaatggcc aaatcttctg gcttgaccag
      241 tagccctggc tacacaggca cagttgagta caagacagat ctggtcgcag ctcacgaact
      301 gctaaacgta acggtggaag aactcggaac aaaacgtggg acgccgctca tggttagcgt
      361 caacgtcaat acgccgtatc cagagttcgt tcccgaagtg aagaaaggct acgtatatgg
      421 cgacttcgaa ctgatcaaag atattttcat gatgcttgaa cttggcatac ctggctattt
      481 gtggggtcat gcaggaacag gcaaatcgtc attgcctaca cagctatgtg ctttgctcaa
      541 tcgtccgttg atccgtgccc aacatacagc atcaatggaa gaggcacatg ttacggggca
      601 aattctggcg cgtgatggct ctacgtattt cgaacctggc ttgcttgcgc tcgcaatgaa
      661 gcatggctgg gtttacctcg cagatgaata cgactttgcg tttccacaaa ttcttggcgt
      721 gtatcagcca gtgctggaag gtgaagcgtt ggtcatcaaa gaggcaactc cagaatggcg
      781 tcgcattact ccgcatgaac ggtttgcttt cattggcact ggcaacacga acggatctgg
      841 tgatgaaacc ggcttgtacc agggtacaaa catccagaac gccgcgaact tttcgcgttt
      901 tggcatcgtt tcgaatgtga aatacatgag caaagacgca gagatcaaca tgttgacaaa
      961 tgcaggcatc gtggatgaat acgctgaaaa gatggttaag tttgccggta tcgttcgcga
     1021 tggatacgaa gaacacctta ttagtcagcc aattggccct cgtgaacttt tgttgtcggc
     1081 caagattgga atgatgcgag gcgactttgt gacaggtatt gagcgttctt tcattaacaa
     1141 actcccttca gcttctgcac aagcggctcg tgaagttgtt caaaaaatat ttggttaatc
     1201 gtgcgtaaag gatgtttcgg ctctcttatc gctgcttctg aaactggtaa ggcttgtctg
     1261 gtgtgtccag acaagcccga ttgtcaccaa tcagcaaaag aagttgcgat ttcgatgcat
     1321 gggaagttcg taggcttccc caatgacaaa atcaaaaaaa ccagaaaggt aaaaacacat
     1381 gaaggcactg atggttcgaa ctgacttctc acttggggag tcggctctaa aagcagaaaa
     1441 cgcggtgaag attgccagag aagctggcta caccgctgta atttcagcag atagcatgaa
     1501 tattgcgagc gttattccac tacaacgtgc cgctggtgac gacatggcgg ttatttgtgg
     1561 tgtgaaacta aacattgttg atgatcccac atacgagcac cgggctaaac ttgctaaaga
     1621 atctatgaga tgtatggaat cattagagcg gggacgtaac tactcgttta ccgctctaat
     1681 taaaaatgag caaggatatc gcgacatctg cgaactaatg acggtggcca acacacgaga
     1741 acagttctac tttgtaccgc gtctctcgct cgaacagttg gtttctacat atgccaaagg
     1801 caacatcatc ctgcttactt ccgacatcgg tagcgtgttc caacgcaacg attttgcaaa
     1861 aatcataagc acactgatta cagcgggtgg aaaagacaac ttctatagtg tggtttatcc
     1921 gcaccctacc ccattctacg accagattaa cgtccgggcg atgaaagtcg ccagcgcact
     1981 gaaaatagag ccagtagcgt tctatcccgc ttattacgaa tcgatcgacg atgcagacat
     2041 taaagacatt gcgcacatgg ttacgaacaa cataaaaatc gaccagccgc atcgtctgcg
     2101 tatcccccac cagcgagata acgccatcaa tggtcgtcgc catctccttg aggcgcttaa
     2161 agccttctcc gttcgcatgg atgtgccggt aacagctgca atggcctcaa caacgcagga
     2221 ctccattatc gatgcctgca catggcgctg gcatgaattg ccaccagcac tgcccaagat
     2281 ggcagacgac gaacctgcaa cgctgatgaa actggctgtt gcagggctgc gtaaacgtct
     2341 taccacaaaa gagtttggat acacaccacc tgcttctgag aacagggttt atgttgagcg
     2401 gctaaagtac gaaatggaca cgctgactcg cctgggattc tgtggttact tcctgatggt
     2461 acgcgatctg atgaatcaca gccgtgaaac tggcattcct gtcgggcctg gtcgtggttc
     2521 ctctgccggt tctctggtgg cgtggtgcat aggcataacc aacgtcgacc caatccgtca
     2581 cggtcttctg tttgagcgtt tcatcaaccc tgagcgtctc gacttgccgg atgcggactt
     2641 ggacttcagc caggcacgtc gccatgaggt gatcgagtat ctgaatgaac gctacggcga
     2701 agattacgtt gcaggcattc cgaacttcac ctacctgggc gcagcctctg cactacgtga
     2761 caccgctcgt atttatggtg tggagtccgc agatatggcg gtatcaaaag aactgaagaa
     2821 cgtcgaggat gatagccttc cattggaaga gctgcgcgaa caactggcaa gtctcgacaa
     2881 atacgcaaca aaatatcctg atgcattcaa tgcagcctgc aagttacaaa gccttatgcg
     2941 tggctttggt agacatgcgg cagggatgat cgtagcaggt gttcctctga cagaacgtac
     3001 accggttgaa cgccgtggtg acgcgcgttg tatcgcattt gacaagcgtt actgcgaggc
     3061 tatgggccta attaagctgg acgtacttgg tctggcaact ctcgatttgc tcgatagtgc
     3121 aaaacgctac ataaaagaga acacaggtga agatatcaat cttgatgcca tttctcttga
     3181 agatcgcaag gtgctggatg gttttgctgc tgggtacact caaggtgttt tccagcttga
     3241 atcaggccca atgcgcaagc tgcttaaaga tttaggtggt ggaattgagc caatgagctt
     3301 taaaacggtt gttgctacga ctgcgctctt ccggccgggg ccaattcagt caggcatgtt
     3361 ggatgactat gtttctgtag ccaaaggctt tatgacgccg gaatcattac accccgttct
     3421 tgatgaactt accgcagaaa caaatggcgt gattctctat caggaacaga cgatgaacgc
     3481 gactcgattg cttgctggct tcacaatggc tgaagctgac ggcgttcgta aagcgatcgg
     3541 caaaaaagac atggaaaaaa tgaagagcat gggcgagaag ttcatcgttc aggctcaagc
     3601 tggctggata gacgttgagc tggaagatgg cactacacag cgcattcacc gtgcggaaca
     3661 ttttaaatgc gaagacggaa ctctgaaaac tgtcgaagag gcacttgagt acggcgcaaa
     3721 actacctata aacgcagtac gcgttacagc gtcacatcca gggctatcag agatgaaagc
     3781 gaaggagatc tggaccgcat tcgaaaagaa tggtgcctac cagttcaaca aatcacactc
     3841 tgttgcttac tccttaatca gttatcaatc tatgtggttg aaaactcatt atcccgcgga
     3901 gttcttcgct gctgctctca ctattcttgg cgaagataaa caccaggggc tggttaaaga
     3961 tgcgctgacc tatggtattc gcgtattgcc accagacgtt aacgtgtcat ctaaccgaat
     4021 cgagatccgc acgcttgaag atggcagcca ggtgctgtat gcgcccttct ctgctgtgaa
     4081 agggtgttct gagaatggct gccaagccat catgagagcg cgagaaaaag ttggcggcaa
     4141 attcgagtca ctggcgcaat tcgaagaagc tgtcgagaaa cgtgcctgta atagtcgagt
     4201 gcgcgaatca ctgcaaaaag taggtgcgtt tgcatccatc gagccaggta gtctgccagc
     4261 aactgatcca gagcgtctcc gcgaccaggc tgaattgatg ggaaaccttg tcatagacgc
     4321 agttaaagcc tcacgtccgt ttgagatgaa ccccaaacgt tcggctgaga ttaacgtgct
     4381 catgacacgg atggcggctg aaatgggatt aggtgatgaa ctaatccgcc ctagcattgg
     4441 cattaagccg aaaatcatga tcattctgga caatgcgaac ggcaatgatg ctcgaaccgg
     4501 ttactttatg gagaacggat acgacgactt taaggccaag ctattgacgg ctggagattt
     4561 acgcatgggc gatctctatg tcacaggcgt ttgcaagaag gttaaagaca aagaaaaaga
     4621 ctacaccaaa gacgagatcg gccagttcac cgactttata cgtgaagaga tcaatctggt
     4681 gcgtccgacc tatgtgctga cgtgtggcag ccgggcaacg gcattgttta acaacaaaaa
     4741 caagccatcc gatttggtcg gcaggaagga gtaccttcca gaccttgacg ttactgtctt
     4801 ttacgggttt aaccccaaca ttctttactt cagacctgaa gagggagaac gactagaagc
     4861 gatattagcc gacgtagcag aaaccattaa gacgtgatcc cataaacatg gcctctatgg
     4921 ccatgttttc ccttatcccc tcatatcccc tatcctcgaa ctccttgcct tatcatcaca
     4981 atgatataat caatataaaa tgataagtaa aaaggaaagc acatgatcac cgatatttac
     5041 gagaaaataa tgtctgatct cgagtttgac cgagacaatc tggaggaagt ctggcgtaga
     5101 caaccccgcc ttttaatgga gtatggctca aaactcgctc atgcagaaag aagtgtcgca
     5161 gaggcaaaac ttaaccttga agctgttgaa gcaaagctat acgacacaga gcgtaagaac
     5221 ttgagtatga acggcattaa gttcaacgag tctgtactgg acgctaaggt taaaacaaac
     5281 ccacagtatc tgtctaaacg gcagaagttg gatgaagcac ggcacatcgc agacatatac
     5341 aaacatgctg tcgccgcctt ttcgcatcgc cgagacatga tcgttcaggc gtcaaagatg
     5401 gccatcgttg aattagagcg attaggctct gaacgcttta ttactccccg ttgatttttg
     5461 atagataata agtaagtact gatctatcat ttaacagctc gaaagagcca cgaatgaacg
     5521 aaagcccaac gcgcatagcg ccatcggcca aatcacaaca aggagaaaca catgtctaag
     5581 acattacttg atttgcttaa caaaactcgt gaagacattg ccgccaaacg tggtaacaac
     5641 gttgatctga ctcgcttaaa agacggcgtc aactatatcc gcatcttccc gaataaagac
     5701 gacccaaacg gtaagttctt ccagactttc ggtatgcact acgttaagta tcagaacgag
     5761 gaaggtaaag aagcaaccaa cgcttatatt tgtgagcaac atactcacgg tcgcgcttgt
     5821 cagctatgcg aaatggtgat ggaaggtcgc gctcgtcaca agggtaacaa agcaatggaa
     5881 gaacgcatcg gtcaaatgcg tgccactcct cgctacctgg tcaacggcat tctttctgct
     5941 cgtgaggatt tcgcagatgc tgagaaatgc cagttaatcg agctgccgtc tactgtattc
     6001 gatgatatct gcaaagcaat caccgaagac atcgctgatg atatcggcaa tccactgagc
     6061 aaagaggaag gctacgcatt cctgattaaa cgtactggct ctggtcgcga taccaaatat
     6121 gacgtctcgc ctaagcgtaa agtctacaaa ggcgatatcg aagataaatt ctggaatacc
     6181 cagcatgatc tgatcgcata cgcaaatcag gctgatgaaa ctcgtcttct gtcgacagtt
     6241 cgcactatgg gtcgtctgat tggcatcgct gcaccaactg ccgcagcatc tgcaccagca
     6301 atttcctcaa ccgcgaaaac atcggctgcg gcactacctg gatttggctc tgtcactggt
     6361 catacagaag gagcgacggc tgtagcaacc gcgcacacac cggcttctga accaaccagt
     6421 ctggttgatg aagaaatcct ccgtgccgtt gaaactgaat ttaaaccaga ggcaagttcc
     6481 gctgccgttg ctgtatcagt caaagagtct gaagcagtcg cagcgacatc tgtagcaacc
     6541 gcatctgcga cggaagatga aggtctggat gacctactga gagagctgga ctctctgtaa
     6601 tcccattacg tgaccagtaa ggcgtctacg gacgccttac tttttggaag gaatgtaccg
     6661 gtgaattatc tcttcgtaga tggcaatagc ctgggttatt accaccaaca atctgacaaa
     6721 ttgcacaacg gcgaaatgga agtacaggct gctttcggct ttgttaagaa cgtccgtcgt
     6781 tatgcctcca tcctccatgc ccgacctatg attctttggg atggatttag tgacaagcgt
     6841 cgcgactttt acccggacta caaagcaaat cgcgacgacg atcctgatat gaaaaagatg
     6901 aaggaaggct ttgctatcca gaagccatac atcctcaaaa tgatgaccgc gcttggtgtt
     6961 aaccaaatca ttgcaaaaga tgcagaagcg gatgatctgg ccgggctgct ggtatcccgc
     7021 atggcaccgc agccaaccgt tgaacacatc tatctgttaa caggcgatag cgactggctt
     7081 cagttagttc gcgaaaacgt aagctgggta agcctgcgtg aagacgccaa aaacaagcag
     7141 gttaattttg agcaatttgc ggagctgaca ggattcgcca cgcctcgcgc atttttggaa
     7201 gcaaaagcat tacaaggcga taactcggac aacattagcg gtgttggtgg cattggtgct
     7261 ggcggtgcga aagagctgct gcatgaatgg ggaagtgtcg caacgatggt acgcggcatc
     7321 aacgacggct caatcgtggt taataaaggg cgtcataaga ccgccttcaa caaactagcg
     7381 aagaatgcct tcaacgagaa aacaggctgt cgaatgctcg aagcgttcaa gagaaacatc
     7441 acgctaatga acctgattga gacaaagttt ccgcctaccg aaatcgaaac aatcaaaggc
     7501 aatcgtgacg tgaaagcatt cgagcaactg tgctacgagc tgaatttccg ttcgttcctt
     7561 gaagaccttg aagtgtttgt tcttccattc gaaaggtatt gctaatgctt aaatcgatta
     7621 tcaatggcgc tacaaccacc cctgcccaac tggcaaaaga gattgtcttt tatcacggtg
     7681 agtacgctgt catcgcactg ccgtcaattc taggcgctgc cggaatgaaa gcgacagatc
     7741 gcgagtttgg attagtcagc gagcaggtcg taaaaatcct cgctcgtgta tccagactcc
     7801 ttaaccacga tgcgattgta ttcgacgaat ccgccgcttt aaaacgaatc aacgaaacaa
     7861 aaggagcctg atcatggcaa aaggaaaatc cgcactggca cttgctctga aaaagaaaat
     7921 cggtagcaac gacgaaattc agaaagtaac tcattggatt gatacaggct ttccaccgtt
     7981 aaacaaagct atttctggtc gttacgatgg cggcttccca tgtggtcgta tcgtcgaagt
     8041 attcggtcct ccaagtgcag ggaaaacgtt ccttgcaaca gcagcgatga tctccgctca
     8101 aaaacaagat ggtctggcgg tattcctcga tcatgaaaac agttttgacg ttggcctggc
     8161 tgtagccaat ggcctgaacg cagatgagga tgacggtcag tgggtataca agcagcctga
     8221 taccttcgaa gactctgtag agttgatcgg cacaattctt aaattggtac gtgatgaaga
     8281 gcttattcct gaatcagcac ctatctgtat cgtggctgac tcacttgcgt ctatggttcc
     8341 gaactccaaa gccgagaagt tcgaaaagat ggctgaaggc actgccaaag acaaagatca
     8401 gctaaacatg aacgacaata cggcactggc tcgtgcgaca agtgcgaact tccctactct
     8461 ggctttgtgg gcacgcaaat acaacgcctg catcatcttc ttgaatcagg ttcgcacaaa
     8521 aatcggtgta atgtttggcg accctactac gtcgccaggt ggagattcac cgaagttcta
     8581 cgcttctgta cgtatccgtc ttggtgcatc ggtgatgaag gatggtaaag agaagatcgg
     8641 ccaggacgta ggcgcagaat gcatcaaaaa caaagttgca ccaccgtatg gcaaatgcac
     8701 ctggaaattc tacttcgatc ctactcgtgg cctcgacgtt atcgaatcgc tcgtcgagta
     8761 catgctggaa gaaggatacc tgccaaagaa cgccagcggg cgagttgaaa ttggtgacaa
     8821 gaaatacacc aaatcgcaga tcgtcgagat gtatcgggag aagccactgg ctgaaatcat
     8881 tgcggctttg caggcaatcg acgaccgaag agcaaaagac aaccccaccg agtcagtaga
     8941 agagtaaaca caaggcgt
//
LOCUS       DAYPHN010000176         8911 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000176 DAYPHN010000000
VERSION     DAYPHN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.176"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            275..490
                     /gene="essD"
                     /locus_tag="ACG0BW_004099"
     CDS             275..490
                     /gene="essD"
                     /locus_tag="ACG0BW_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFK7260999.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            490..987
                     /gene="rrrD"
                     /locus_tag="ACG0BW_004100"
     CDS             490..987
                     /gene="rrrD"
                     /locus_tag="ACG0BW_004100"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFK7261000.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1204..1386
                     /gene="rzoD"
                     /locus_tag="ACG0BW_004101"
     CDS             1204..1386
                     /gene="rzoD"
                     /locus_tag="ACG0BW_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFK7261001.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1477..1770)
                     /gene="iss"
                     /locus_tag="ACG0BW_004102"
     CDS             complement(1477..1770)
                     /gene="iss"
                     /locus_tag="ACG0BW_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFK7261002.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2133..2327)
                     /locus_tag="ACG0BW_004103"
     CDS             complement(2133..2327)
                     /locus_tag="ACG0BW_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFK7261003.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2716..3261
                     /locus_tag="ACG0BW_004104"
     CDS             2716..3261
                     /locus_tag="ACG0BW_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFK7261004.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3236..5161
                     /locus_tag="ACG0BW_004105"
     CDS             3236..5161
                     /locus_tag="ACG0BW_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFK7261005.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5158..5364
                     /locus_tag="ACG0BW_004106"
     CDS             5158..5364
                     /locus_tag="ACG0BW_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFK7261006.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5361..6962
                     /locus_tag="ACG0BW_004107"
     CDS             5361..6962
                     /locus_tag="ACG0BW_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFK7261007.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6943..8262
                     /locus_tag="ACG0BW_004108"
     CDS             6943..8262
                     /locus_tag="ACG0BW_004108"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFK7261008.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTEVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8272..8604
                     /locus_tag="ACG0BW_004109"
     CDS             8272..8604
                     /locus_tag="ACG0BW_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFK7261009.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8660..>8911
                     /locus_tag="ACG0BW_004110"
     CDS             8660..>8911
                     /locus_tag="ACG0BW_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFK7261010.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHLA"
BASE COUNT         2123 a         2212 c         2540 g         2036 t
ORIGIN      
        1 tttgtggtgg tgttttgaac actcggtggc attctcacaa atatcattta gtagtttacg
       61 tacgtaaaaa attggttatg ctgttaagag tggttacttc gtcacacagc ttaaacccgc
      121 cgtcgagcgg gtttttccat tttttgagtc tcgatattag ctgataaccc aatacctgag
      181 ttattcactg actccgaatc tgttacgttt ctgcctttat tgcgatacgt aatatcccct
      241 taatttacac ccgctttgtc tgcgaggtgg ggttatgaaa tccatggata agttaacaac
      301 gggtgtcgcc tatggcacct cagcaggtag tgccggttac tggtttttac agctgctcga
      361 taaagtcacg ccctcacagt gggcagcaat aggtgtgctg ggtagcctgg tatttggcct
      421 gctgacgtac ctgacaaacc tttatttcaa gattaaagaa gataagcgca aggctgcgag
      481 aggtgaataa tgcctccatc attacgaaaa gccgttgctg ctgctattgg tggcggagca
      541 attgctatag catcagtgtt aatcactggc ccaagtggta acgatggtct ggaaggtgtc
      601 agctacatac catacaaaga tattgttggt gtatggactg tatgtcacgg gcatacagga
      661 aaagacatca tgctcggtaa aacgtatacc aaagcagaat gcaaagccct cctgaataaa
      721 gaccttgcca cggtcgccag acaaattaac ccgtacatca aagtcgatat accggaaaca
      781 atgcgcggcg ctctttactc attcgtttac aacgtgggtg ctggcaattt cagaacatcg
      841 acgcttcttc gcaaaataaa ccagggcgat atcaaaggcg catgtgatca gctacgtcgc
      901 tggacatatg ctggcggtaa gcaatggaaa ggtctcatga ctcgtcgtga gattgagcgt
      961 gaaatctgtt tgtggggtca gcaatgaaca gagtaaccgc gattatctcc gctctggtta
     1021 tctgcatcat cgtctgcctg tcatgggctg ttaatcatta ccgtgataac gccattacct
     1081 acaaagccca gcgcgacaaa aatgccagag aactgaagct ggcgaacgcg gcaattactg
     1141 acatgcagat gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag
     1201 ctgatgcgaa agctgaaaat gatgctctgc gtgatgatgt tgccgctggt cgtcgtcggt
     1261 tgcacatcaa agcagtctgt cagtcagtgc gtgaagccac caccgcctcc ggcgtggata
     1321 atgcagcctc cccccgactg gcagacaccg ctgaacggga ttatttcacc ctcagagaga
     1381 ggctgatcac tatgcaaaaa caactggaag gaacccagaa gtatattaat gagcagtgca
     1441 gatagagctg cccatatcga tgggcaactc atgcaattat tgtgagcaat acacacgcgc
     1501 ttccagcgga gtataaatgc ctaaagtaat aaaaccgaga aatccattta cgaatgtttg
     1561 ctgggtttct gttttaacaa cattttctgc gccgccacaa attttggctg catcaacagt
     1621 tttctcctgt ccaattcccg aaacgaagaa atgatgggtg atggtttcct ttggtgttac
     1681 tgctgtcggt ttgtttccaa cagtaaacgt ctgttgagca catcctgtaa taagcattgc
     1741 cagagcggca gaaaacaaca tttttttcat cttattatcc tgcattgtta aaaacggcag
     1801 aatcctatgt gacaacaatt aaacgatagt taaatggatt gatgaaaatt aaaactatat
     1861 aggtggatgc tcagcctatt ggaggagggg gggggcactc agaatcctgt ggaatgaaat
     1921 aaaccgctct atctgtccat taccctttta gctgcgctgt atcgtcgccg tattcccgca
     1981 ttaaccatga ccgtagcccg acggggaatt ccttctgcgt gagtgtgcgg gaataatcaa
     2041 aaacgatgca caccgggttt tactgtgctg acagacgcag ggttaccctc atagtcgctt
     2101 ttccggtgcg atggtggaag aaaccgggat gttcatccat catcactttg gattgatgta
     2161 tatgctctct tttctgacgt tagtctccga cggcaggctt caatgaccca ggctgagaaa
     2221 ttcccagacc ctttttgctc aagagcgatg ttaatttgtt caatcatttg gttaggaaag
     2281 cggatgttgc gggttgttgt tctgcgggtt ctgttcttag ttgacatgag gttgccccgt
     2341 attcagtgtc gctgatttgt attgtctgaa gttgttttta cgttaagttg atgtagatca
     2401 attaatacga tacctgcgtc ataattgatt atttgacgtg gtttgatggc gtagatgcac
     2461 gttgtgacat gtagatgata attattatca ttttgcgggt cctttccggc gatccgacag
     2521 gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg gtttaaggcg
     2581 tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct gaaaagaaag
     2641 gaaacgacag gtgctgaaaa cgggcttttt ggcctctgtc gtttcctttc tctgtttttg
     2701 tccgtggaat gaacaatgga agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt
     2761 atccgtacca ttcagaactg gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag
     2821 ggtaatgagg tgctttatga ctctgccgcc gttataaaat ggtatgccga aagggatgct
     2881 gaaattgaga acgaaaagct gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca
     2941 gatctccagc cagggactat tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac
     3001 gcacaggagc tgaaaaatgc cagagactcc gctgaagtgg tggaaaccgc attctgtact
     3061 ttcgtgctgt cgcggatcgc aggtgaaatt gccagtattc tcgacgggat ccccctgtcg
     3121 gtgcagcggc gttttccgga actggaaaac cgacatgttg atttcctgaa acgggatatc
     3181 atcaaagcca tgaacaaagc agccgcgctg gatgaactga taccggggtt gctgagtgaa
     3241 tatatcgaac agtcaggtta acaggctgcg gcattttgtc cgcgccgggc ttcgctcact
     3301 gttcaggccg gagccacaga ccgccgttga atgggcggat gccaattact atctcccgaa
     3361 agaatccgca taccaggaag ggcgctggga aacactgccc tttcagcggg ccatcatgaa
     3421 tgcgatgggc agcgactaca tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta
     3481 ttccaaaatg ctgttgggtg tttatgccta cttcatagag cataagcagc gcaacaccct
     3541 tatctggttg ccgacggatg gtgatgccga gaactttatg aaaacccacg ttgagccgac
     3601 catccgcgat attccgtcgc tgctggcgct ggccccgtgg tatggcaaaa agcaccggga
     3661 taacacgctc accatgaagc gtttcaccaa tggtcgtggt ttctggtgcc tgggcggtaa
     3721 agcggcaaaa aactaccgtg aaaagtcagt ggatgtggcg ggttatgatg aacttgctgc
     3781 ctttgatgag gatattgaac aggaaggctc tccgacgttc ctgggtgaca agcgtattga
     3841 aggctcggtc tggccaaagt ccatccgtgg ctccacgccc aaagtgagag gcacctgtca
     3901 gattgagcgt gcagccagtg aatccccgca ttttatgcgt tttcatgttg cctgcccgca
     3961 ctgcggggag gagcagtacc ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg
     4021 gacgccggat gatccctcca gcgtgtttta tctctgcgaa cataatgcct gcgtcatccg
     4081 ccagcaggag ctggacttta ctgatgcccg ttatatctgc gaaaagaccg ggatctggac
     4141 ccgtgatggc attctctggt tttcgtcatc cggtgaagag attgagccgc cggacagtgt
     4201 gacctttcac atctggacgg cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga
     4261 ctggatgaag acgaaagggg atacgggaaa acgtaaaacc ttcgtgaaca ccacgctcgg
     4321 tgagacatgg gaagcgaaaa tcggcgaacg tccggatgct gaggtgatgg cggagcggaa
     4381 agagcattat tcagcgcccg ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc
     4441 ccagctggac cgctacgaaa tgcgcgtatg gggatggggg ccgggtgagg aaagctggct
     4501 gattgaccgg cagattatta tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga
     4561 tgaggccatc aataaaacct atacccgccg gaatggtgca gaaatgtcga tatcccgtat
     4621 ctgctgggat actggcggga ttgacccgac cattgtgtat gaacgctcga aaaaacatgg
     4681 gctgttccgg gtgatcccca ttaaaggggc atccgtctac ggaaagccgg tggccagcat
     4741 gccacgtaag cgaaacaaaa acggggttta ccttaccgaa atcggtacgg ataccgcgaa
     4801 agagcagatt tataaccgct tcacactgac gccggaaggg gatgaaccgc ttcccggtgc
     4861 cgttcacttc ccgaataacc cggatatttt tgatctgacc gaagcgcagc agctgactgc
     4921 tgaagagcag gtcgaaaaat gggtggatgg caggaaaaaa atactgtggg acagcaaaaa
     4981 gcgacgcaat gaggcactcg actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat
     5041 ttcccgctgg cagctggatc tcagtgcgct gctggcgagc ctgcaggaag aggatggtgc
     5101 agcaaccaac aagaaaacac tggcagatta cgcccgtgcc ttatccggag aggatgaatg
     5161 acgcgacagg aagaacttgc cgctgcccgt gcggcactgc atgacctgat gacaggtaaa
     5221 cgggtggcaa cagtacagaa agacggacga agggtggagt ttacggccac ttccgtgtct
     5281 gacctgaaaa aatatattgc agagctggaa gtgcagaccg gcatgacaca gcgacgcagg
     5341 ggacctgcag gattttatgt atgaaaacgc ccaccattcc cacccttctg gggccggacg
     5401 gcatgacatc gctgcgcgaa tatgccggtt atcacggcgg tggcagcgga tttggagggc
     5461 agttgcggtc gtggaaccca ccgagtgaaa gtgtggatgc agccctgttg cccaacttta
     5521 cccgtggcaa tgcccgcgca gatgatctgg tacgcaataa cggctatgcc gccaacgcca
     5581 tccagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     5641 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     5701 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     5761 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     5821 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     5881 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     5941 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     6001 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgt gcctcgttca
     6061 ttcacgtttt tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg
     6121 tgatggagca gatgaagatg ctcgacacgc tgcagaacac gcagctgcag agcgccattg
     6181 tgaaggcgat gtatgccgcc accattgaga gtgagctgga tacgcagtca gcgatggatt
     6241 ttattctggg tgcgaacagt caggagcagc gggaaaggct gacgggctgg attggtgaaa
     6301 ttgccgcgta ttacgccgca gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga
     6361 tgccgggtga ctcactgaac ctgcagacgg ctcaggacac ggataacggc tactccgttt
     6421 ttgagcagtc actgctgcgg tatatcgctg ccggactggg tgtctcgtat gagcagcttt
     6481 cccggaatta cgctcagatg agctactcca cggcacgggc cagcgcgaac gagtcgtggg
     6541 cgcactttat ggggcggcga aaattcgtcg catcccgtca ggcgagccag atgtttctgt
     6601 gctggctgga agaggccatc gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca
     6661 gttttcagga agcccgcagt gcctggggga actgtgactg gataggctcc ggtcgtatgg
     6721 ccatcgatgg tctgaaagaa gtacaggaag cggtgatgct gatagaagcc ggactgagca
     6781 cctacgagaa agagtgcgca aaacgcggcg acgactatca ggaaattttt gcccagcagg
     6841 tccgtgaaac gatggagcgc cgtgcagccg gtcttaaacc gcccgcctgg gcggctgcgg
     6901 catttgaatc cgggctgcga caatcaacag aggaggagaa gagtgacagc agagctgcgt
     6961 aatctcccgc atattgccag tatggccttt aatgagccgc tgatgcttga acccgcctat
     7021 gcgcgggttt tcttttgtgc gcttgcaggc cagcttggga tcagccgcct gacggatgcg
     7081 gtatccggcg acagcctgac tgccggagag gcacccgcgg cgctggcgtt atccgttgat
     7141 gatgacggac cacgacaggc ccgcagttat caggtcatga acggcatcgc cgtgctgccg
     7201 gtgtccggca cgctggtcag tcggacgcga gcgctgcagc cgtattcggg gatgaccggt
     7261 tacaacggca ttatcgcccg tctgcaacag gctgccagcg acccgatggt ggacggcatt
     7321 ctgctcgata tggatacgcc aggcgggatg gtggcagggg catttgactg cgctgacatc
     7381 atcgcccgtg tgcgtgacat aaagccggta tgggcgctgg ccaacgacat gaactgcagt
     7441 gcaggtcagt tgcttgccag tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca
     7501 ggctccatcg gcgtcatgat ggctcacagt aattacggtg ctgcgctgga gaaacagggt
     7561 gtggaaatca cgctgattta cagcggcagc cataaggtgg atggcaaccc ctacagccat
     7621 cttccggatg acgtccggga gacactgcag tcccggatgg acgcaacccg ccggatgttt
     7681 gcacagaagg tgtcggcata taccggcctg tccgtgcagg ctgtgctgga taccgaggct
     7741 gcagtgtaca gcggtcagga ggccattgat gccggactgg ctgatgaact tgttaacagt
     7801 accgatgcga tcaccgtcat gcgtgatgca ctggatgcac gtaaatcccg tctctcagga
     7861 gggcgaatga ccaaagagac tcaatcaaca actgtttcag ccactgcttc gcaggctgac
     7921 gttactgagg tggtgcaagc gacggagggc gagaacgcca gcgcggcgca gccggacgtg
     7981 aacgcgcaga tcaccgcagc ggttgcggca gaaaacagcc gcattatggg gatcctcaac
     8041 tgtgaggagg ctcacggacg cgaagaacag gcacgcgtgc tggcagaaac ccccggtatg
     8101 accgtgaaaa cggcccgccg cattctggcc gcagcaccac agagtgcaca ggcgcgcagt
     8161 gacactgcgc tggatcgtct gatgcagggg gcaccggcac cgctggctgc aggtaacccg
     8221 gcatctgatg ccgttaacga tttgctgaac acaccagtgt aagggatgtt tatgacgagc
     8281 aaagaaacct ttacccatta ccagccgcag ggcaacagtg acccggctca taccgcaacc
     8341 gcgcccggcg gattgagtgc gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc
     8401 agccgtaagc tggttgcgtg ggatggcacc accgacggtg ctgccgttgg cattcttgcg
     8461 gttgctgctg accagaccag caccacgctg acgttctaca agtccggcac gttccgttat
     8521 gaggatgtgc tctggccgga ggctgccagc gacgagacga aaaaacggac cgcgtttgcc
     8581 ggaacggcaa tcagcatcgt ttaactttac ccttcatcac taaaggccgc ctgtgcggct
     8641 ttttttacgg gattttttta tgtcgatgta cacaaccgcc caactgctgg cggcaaatga
     8701 gcagaaattt aagtttgatc cgctgtttct gcgtctcttt ttccgtgaga gctatccctt
     8761 caccacggag aaagtctatc tctcacaaat tccgggactg gtaaacatgg cgctgtacgt
     8821 ttcgccgatt gtttccggtg aggttatccg ttcccgtggc ggctccacct ctgaatttac
     8881 gccgggatat gtcaaaccca agcacttagc a
//
LOCUS       DAYPHN010000177         8840 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000177 DAYPHN010000000
VERSION     DAYPHN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.177"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            22..543
                     /gene="fecI"
                     /locus_tag="ACG0BW_004111"
     CDS             22..543
                     /gene="fecI"
                     /locus_tag="ACG0BW_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFK7261011.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            540..1493
                     /gene="fecR"
                     /locus_tag="ACG0BW_004112"
     CDS             540..1493
                     /gene="fecR"
                     /locus_tag="ACG0BW_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFK7261012.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSTSEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1580..3904
                     /gene="fecA"
                     /locus_tag="ACG0BW_004113"
     CDS             1580..3904
                     /gene="fecA"
                     /locus_tag="ACG0BW_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFK7261013.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            3949..4851
                     /gene="fecB"
                     /locus_tag="ACG0BW_004114"
     CDS             3949..4851
                     /gene="fecB"
                     /locus_tag="ACG0BW_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFK7261014.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4848..5846
                     /gene="fecC"
                     /locus_tag="ACG0BW_004115"
     CDS             4848..5846
                     /gene="fecC"
                     /locus_tag="ACG0BW_004115"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFK7261015.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5843..6799
                     /gene="fecD"
                     /locus_tag="ACG0BW_004116"
     CDS             5843..6799
                     /gene="fecD"
                     /locus_tag="ACG0BW_004116"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFK7261016.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHHRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6800..7567
                     /gene="fecE"
                     /locus_tag="ACG0BW_004117"
     CDS             6800..7567
                     /gene="fecE"
                     /locus_tag="ACG0BW_004117"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFK7261017.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8124..8381)
                     /locus_tag="ACG0BW_004118"
     CDS             complement(8124..8381)
                     /locus_tag="ACG0BW_004118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261018.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8464..8840)
                     /locus_tag="ACG0BW_004119"
                     /pseudo
     CDS             complement(8464..8840)
                     /locus_tag="ACG0BW_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615349.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1888 a         2543 c         2470 g         1939 t
ORIGIN      
        1 ttaacttttg atgcactccg catgtctgac cgcgccacta ccacagcttc cttaacgttc
       61 gagtcgcttt atggcacaca tcacggctgg ttgaaaagct ggctgacgcg caaactccag
      121 tctgcttttg atgcagatga cattgcccag gacacttttt tgcgggtaat ggtcagcgaa
      181 acgctctcga cgatccgcga tcctcgctcc ttcctctgca ctatcgccaa acgcgtgatg
      241 gtggacctgt ttcgccgaaa cgcgctggaa aaagcgtatc tggagatgct ggcgcttatg
      301 ccggaggggg gagcgccttc acctgaggaa cgcgaaagcc aactcgagac cctacaactc
      361 ctcgacagca tgctggacgg gctaaacggc aaaacacgtg aagcgtttct gctttcgcaa
      421 ctggatggtc tgacatacag cgagattgcg cacaaactcg gtgtttccat cagctccgtg
      481 aaaaaatacg tggcgaaagc cgtcgagcac tgcctgctgt tccgtctgga gtatgggtta
      541 tgaatccttt gttaaccgat tcccgccgtc aggcgctgcg ttcagcttcc cactggtatg
      601 ccgtgctaag cggcgagcgc gtcagcccac aacaggaagc gcgctggcaa cagtggtatg
      661 aacaggatca ggataaccag tgggcctggc agcaggttga aaacctgcgc aaccagcttg
      721 gcggtgtgcc tggcgacgtt gccagccggg cgttgcacga tacccgcctc acccgccgtc
      781 acgtgatgaa aggattactg ctgttgctcg gcgctggcgg aggctggcag ctctggcagt
      841 cggaaaccgg cgaaggtctg cgggcagatt accgcaccgc caaaggcaca gtcagccgtc
      901 agcaactgga agatggctcc ctgctcacgc tgaataccca aagcgcggcg gatgtgcgtt
      961 ttgatgcgca tcagcgcacc gtccggctct ggtacggtga aatcgccatt actaccgcga
     1021 aagatgccct gcaacgcccc ttccgtgtcc tgacccgtca gggccagctc actgctttag
     1081 ggacagaatt taccgtccgc cagcaggata atttcacgca gcttgacgtt cagcagcacg
     1141 ctgtggaagt gcttctcgcc agtgcccccg cgcaaaaacg catcgtgaac gctggtgaaa
     1201 gcctgcagtt cagcacctct gagtttggcg cagtgaaacc gctggatgac gagagtacaa
     1261 gctggacgaa ggacatcctg agcttcagcg ataaaccact gggtgaggtg atagccacgc
     1321 taacccgtta ccgcaacggc gtgctgcgct gcgatcccgc cgttgccggg ctgcgcctga
     1381 gcgggacgtt cccgctgaaa aataccgatg cgatcctgaa cgttatcgcg caaacgcttc
     1441 ccgttaaaat tcagtctatt acgcggtact ggataaacat ttcaccactg taaggaaaat
     1501 aattcttatt tcgattgtcc tttttaccct tctcgttcga ctcatagctg aacacaacaa
     1561 aaatgatgat ggggaaggta tgacgccgtt acgcgttttt cgtaaaacaa cacctttggt
     1621 taacgccatt cgcctgagcc tgctgccgct ggccggtctc tcgttttccg cttttgctgc
     1681 acaggttaat atcgcaccgg gatcgctcga taaagcgctc aatcagtatg ccgcacacag
     1741 cggatttacc ctctcggttg acgccagcct gacgcgcggc aagcagagca acggcctgca
     1801 cggcgattac gacgtcgaga gcggcctgca acaactgctg gacggcagcg gactgcaggt
     1861 aaaaccgctg ggaaataata gctggacgct ggagcccgcg cccgcaccaa aagaagatgc
     1921 cctgaccgtg gtcggcgact ggctgggtga tgcgcgtgaa aacgacgtat ttgaacatgc
     1981 tggcgcgcgt gacgtgatcc gccgtgagga tttcgccaaa accggcgcaa ccaccatgcg
     2041 tgaggtactt aaccgcatcc ctggcgtcag cgcgccggaa aacaacggca ccggcagcca
     2101 cgacctggcg atgaactttg gcatccgggg cctgaacccg cgcctcacca gccgctcgac
     2161 cgtcctgatg gacggcatcc ccgtcccctt cgccccttac ggtcagccgc agctttcact
     2221 ggctcccgtt tcgctcggca acatggatgc cattgacgtg gtacgcggtg gtggtgcggt
     2281 gcgttacgga ccgcagagcg tgggcggcgt ggtgaacttt gttacccgtg ccattccgca
     2341 ggactttggt atcgaggcgg gcgtggaagg tcagctcagc ccaacctctt cacaaaacaa
     2401 cccgaaagag acgcacaacc tgatggtggg cggcacagcg gacaacggtt ttggcaccgc
     2461 gctgctctac tccggcacgc gcggcagtga ctggcgcgag cacagcgcca cccgcatcga
     2521 cgacctgatg ctgaaaagca aatatgcgcc ggatgaggtg cacaccttca acagcctgct
     2581 gcaatattac gacggtgaag ccgacatgcc cggtggcctg tctcgcgcgg attacgacgc
     2641 cgatcgctgg caatccaccc gcccgtatga ccgcttctgg ggtcgtcgca agctggcgag
     2701 cctgggctac cagttccagc cagacagcca gcataaattc aacattcagg ggttctacac
     2761 ccaaaccctg cgcagcggct acctggagca aggcaaacgc atcaccctct cgccgcgtaa
     2821 ctactgggtg cgcggtattg agccacgcta cagccagatc tttatgatcg gcccttccgc
     2881 gcacgaagtg ggcgtgggct atcgctatgt gaatgaatca acgcatgaaa tgcgttacta
     2941 caccgccacc agcagcgggc agttgccgtc cggctcaagc ccttacgacc gcgatacgcg
     3001 ttccggcacc gaggcgcacg cctggtatct ggatgacaaa atcgacatcg gcaactggac
     3061 catcacgccg ggtatgcgtt tcgaacatat cgagtcatac cagaacaacg ccatcacagg
     3121 cacgcacgaa gaagtgagct ataacgcacc gcttccggcg ttgaacgtgc tctatcacct
     3181 gactgacagc tggaatcttt atgcaaacac tgaaggctcg ttcggcaccg tacagtacag
     3241 ccagattggc aaggctgtgc aaagcggcaa tgtggaaccg gaaaaagcgc gaacctggga
     3301 actcggtacc cgctacgacg acggcgcgct gacggcggaa atggggctgt tcctgattaa
     3361 ctttaacaat cagtacgact ccaaccagac caacgacacc gtcactgcac gtggcaaaac
     3421 gcgccataca gggctggaaa cgcaggcacg ttacgatctg ggtacgctaa cgccaacgct
     3481 tgataacgtt tccatctacg ccagctatgc gtatgtgaac gcggaaatcc gcgagaaagg
     3541 cgacacctac ggcaatctgg taccattctc cccgaaacat aaaggcacgc tgggcgtgga
     3601 ctacaagcca ggaaactgga cgttcaatct gaacagcgat ttccagtcca gccagtttgc
     3661 ggataacgcc aatacggtga aagagagcgc cgacggcagt accggccgca ttcccggctt
     3721 catgctctgg ggcgcacgcg tggcgtatga ctttggcccg cagatggcag atctgaacct
     3781 ggcattcggt gtgaaaaaca tcttcgacca ggactacttc atccgctctt atgacgacaa
     3841 caacaaaggc atctatgcag gccagccgcg cacgctgtat atgcaggggt cgttgaagtt
     3901 ctgatgtgtt aacgcccggc ttgccgggct tttagctgga atgtgattat gttggcattt
     3961 atccgttttg tttttgcagg cctgctgctg gtgatcagcc acgcctttgc cgccacggtt
     4021 caggacgaac acggcacgtt tacactcgaa aaaacgccac aacggattgt ggtgctggaa
     4081 ctctcgttcg ccgatgcgct ggccgccgtg gacgtcagcc cgatcggtat tgccgacgat
     4141 aacgatgcaa aacgcatcct gcccgaagtg cgtgcgcacc tgaaaccgtg gcagtccgtc
     4201 ggaacgcgcg cgcagccgag cctggaagcc attgccgctc tgaaaccaga cctgatcatt
     4261 gccgacagca gtcgccatgc gggggtttac atcgccttgc agcaaatcgc gccggtactg
     4321 ctgcttaagt cccgcaacga aacctacgct gaaaatttgc aatctgcggc tatcatcggc
     4381 gaaatggtgg gtaaaaagcg agagatgcag gcacgcctgg aacaacataa agagaggatg
     4441 gcgcagtggg ccagccagct tcccaaaggg acacgcgtgg cctttggcac atcacgcgaa
     4501 cagcaattca acctgcatac tcaggagacc tggaccggca gcgtgctggc ctctctgggg
     4561 ctgaacgttc ccgctgcgat ggcgggtgcg tccatgccgt ccatcggcct ggagcaactg
     4621 ctggcggtca atcctgcctg gctgctggtt gcccactatc gcgaagagag cattgttaaa
     4681 cgctggcaac aagatccgct ctggcagatg ttaaccgccg cgcagaagca gcaggttgct
     4741 tcggtcgaca gtaacacctg ggcgcggatg cgcggtattt ttgctgcaga gcgtattgcc
     4801 gctgacacgg taaaaatctt ccaccatcag ccgcttaccg ttgtgaaatg accgcgataa
     4861 aacacccggt gctgctgtgg gggcttcccg ttgcagcact tattattatt ttctggctga
     4921 gtctgttttg ctactcggcc attcctgttt ccggagcaga tgcaacccgc gccctgctgc
     4981 ctggacacac gccaacgcta ccagaagcgc tggtgcaaaa ccttcgtttg ccacgaagcc
     5041 tggtcgccgt tctgatcggc gcaagcctgg cgctcgcggg cacgctgctg caaaccctga
     5101 cccacaaccc aatggcctct ccttcactgc tcggcattaa cagcggcgcg gcgctggcta
     5161 tggcgcttac cagcgcgctg agtccgacgc cgattgcagg ctattctctg tcgttcatcg
     5221 cggcatgcgg gggcggcgtg agctggctgc tggtcatgac cgcaggaggc ggatttcgcc
     5281 atacccatga cagaaacaaa ctgatcctcg cgggtatcgc gctgtcggcc ttttgtatgg
     5341 gccttacccg catcaccctg ctgctggccg aagatcatgc ttacggcatc ttttactggc
     5401 tggcaggcgg agtgtcccac gcccgctggc aggatgtctg gcagctcttg ccggtggtgg
     5461 tcactgcagt ccctgtcgtg ttgctgctgg cgaatcaact gaacctgctc aacctcagcg
     5521 acagcaccgc ccatacgctg ggagtgaacc tgacgaggct acgtttggtc atcaatatgt
     5581 tagtgctgct tctggttggt gcgtgcgtca gcgtggcagg tccggtggcg tttatcggtc
     5641 tgctggtgcc acatctggcg cgcttctggg caggcttcga tcagcgcaac gtactgccag
     5701 tgagcatgct gctgggggcc acgctgatgc tgctggcaga tgtactcgca cgcgcactgg
     5761 ccttccccgg agatctgccc gcaggcgcag tgctggcgct gattggcagc ccttgctttg
     5821 tctggcttgt gaggaggcga ggatgaaaat tgcgctggtt attttcatca cccttgccct
     5881 ggcgggctgt gcgctgttat cactccatat gggagtgatc cccgtgccgt ggcgcgcgct
     5941 gctgaccgac tggcaggccg gacgcgagca ttattatgta ttgatggagt accgactgcc
     6001 gcgcttgctg ctggcactgt ttgtcggtgc agccctcgcc gtggcgggcg tgctgataca
     6061 ggggattgtg cgcaaccctc tggcatcacc ggatattctc ggtgttaacc atgccgccag
     6121 cctggcctct gtgggggctc tacttcttat gccgtcactg cccgtgatgg tgctgccgct
     6181 gctggccttt gcgggcggca tggcggggtt gatattgctg aagatgctgg caaagaccca
     6241 ccagccgatg aagctggcgc tcaccggcgt ggcgctttct gcatgctggg ccagcctgac
     6301 ggattatctg atgctctcgc gcccgcagga tgtgaacaac gccctgctgt ggctgaccgg
     6361 cagcttatgg ggccgtgact ggagctttgt gaagattgcc atcccgctga tgattttatt
     6421 tctgccgctg agcctgagtt tttgccgcga tctcgacctc cttgcactcg gcgatgcgcg
     6481 cgccaccacg ctcggtgtgt cggtgcccca tacccgattc tgggctttgt tactagctgt
     6541 cgccatgaca tctaccggcg tggccgcctg cggcccgatt agctttattg gtctcgtggt
     6601 gccgcatatg atgcgtagca tcaccggtgg acgtcaccac agactgctgc ctgtttcggc
     6661 cctgacaggt gcgttgctgt tggtggttgc cgatctgctg gcgagaatta ttcatccccc
     6721 actggagctc ccggttggcg tgctgaccgc cattatcggt gcgccgtggt ttgtctggtt
     6781 gcttgtgaga atgcgataaa tgactttacg aactgaaaat ctgacggtca gttacgggac
     6841 agacaaggta cttaacgacg tttcactctc actgccaacg gggaagatca ccgccctgat
     6901 cggtcctaac ggttgcggga aatcgacgct gttaaactgt ttttcgcggc ttttaatgcc
     6961 gcagtctggc accgtatttc tcggcgataa tcccataaat atgctctcat cgcgccagtt
     7021 ggcccgcagg ctttcgctgc tgcctcagca ccatttaacg ccagagggga tcacagtcca
     7081 ggagctggtt tcgtatggtc gtaatccctg gctgtcactc tgggggcgtc tctccgctga
     7141 agacaatgca cgagttaatg tcgccatgaa ccagacccgg atcaatcatc ttgccgttcg
     7201 tcggttaacc gagctttccg gcggtcagcg ccagcgcgca tttctggcaa tggtcctggc
     7261 ccagaatacg cccgttgtat tacttgatga gccaaccacc tatcttgata tcaatcacca
     7321 ggtggacctg atgcggttga tgggcgaact ccggactcag gggaaaacgg tggtcgctgt
     7381 gctgcacgac cttaatcagg ctagccggta ctgcgatcaa ctggtggtaa tggcaaacgg
     7441 acatgttatg gcgcaaggca caccagaaga ggtgatgacc ccaggattgc tgagaacagt
     7501 attcagcgtg gaagcggaaa tacaccccga gccggtatct ggcaggccga tgtgcctaat
     7561 gaggtagatt gcacaggccg taagaaccaa accacgactg aatgaaactg gactggcgcc
     7621 agcaagcctg ttcagactgg ggctgaactt ttccggactc tgaaagatta ccaatactca
     7681 tcgtccatcc gcttgcttta ggctgacagg ttcataatca acgcaaacca gagctgtaca
     7741 ggcttgggcg cggctttcaa accagtcgtg atcacggcaa tcaattttga actctgctta
     7801 acggacattt ctgtataacc cttacggcaa cgaaaaacgc gaagttaaaa ttttagaaac
     7861 ccaaaaacgt gacatgacta agtttagatt tcagggggga gatcaaaaaa tttcgctctg
     7921 tgccagagcg gacattcacg gagctggttc attaccaatg aggttgggct tttgaggata
     7981 aatcaatgat cagacgccaa cgtaaatcaa aagcaaccct ggaacggaat tgctaatcca
     8041 gtttctgacc atcgattttt ctaaaaagtg tgcgtttgct actacttagg taggtgcagc
     8101 tttcttaatc accggcagcc acgttataca ggccagttga tggatcgatt gttatcaatg
     8161 atatcttcat gagtcggtgt ctcacccagc ttaccgaagc tggcataaag tgaaggcaga
     8221 cgggcccgtc cttctccctt tttcgccaga gggaaagcgc gaagcatggt ggcagcctcc
     8281 cagtcacaac aataggatgg tgtgcacggc tgctgacgcc atgattcagc gatagagccg
     8341 gaaaatacgg ggtcaaagcc ggtatcatta accagagtca tcgtgacctg tactgcggct
     8401 ggatgatctc ctgctactgc aactgccagg cgaccgcggc tcccttcagg tagtctgttg
     8461 tggtcaacta aaactggcct ccgcgttaga gtttttccag tatcggtttt ctgattcgtt
     8521 tggtggtaac ccaccattat attcgtgcgg tcttagtgcg ctgtaatatc caacgatata
     8581 gtccgttatt gcgtgagctg catcgctgaa gcttacatag cccgtcgctg gcacccattc
     8641 gttcttcaga ctcctgaaga agcgctccat tgggctgtta tcccagcagt ttccacgccg
     8701 actcatactc tgcctgatcc ggtatcgcca cagtaactgc cggaactgcc tgctcgtata
     8761 atgactgcct tgatcgcttg gaacatcacc ccgacgggct taccacgggt ttcccatgcc
     8821 atttccagtg ctttcatggt
//
LOCUS       DAYPHN010000178         8760 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000178 DAYPHN010000000
VERSION     DAYPHN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.178"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            404..946
                     /gene="ecpR"
                     /locus_tag="ACG0BW_004120"
                     /gene_synonym="matA"
     CDS             404..946
                     /gene="ecpR"
                     /locus_tag="ACG0BW_004120"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFK7261019.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVP"
     gene            1021..1608
                     /gene="ecpA"
                     /locus_tag="ACG0BW_004121"
                     /gene_synonym="matB"
     CDS             1021..1608
                     /gene="ecpA"
                     /locus_tag="ACG0BW_004121"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFK7261020.1"
                     /translation="MKKKVLAIALVTVFTGSGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1666..2334
                     /gene="ecpB"
                     /locus_tag="ACG0BW_004122"
                     /gene_synonym="yagY"
     CDS             1666..2334
                     /gene="ecpB"
                     /locus_tag="ACG0BW_004122"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFK7261021.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2360..4885
                     /gene="ecpC"
                     /locus_tag="ACG0BW_004123"
                     /gene_synonym="yagX"
     CDS             2360..4885
                     /gene="ecpC"
                     /locus_tag="ACG0BW_004123"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFK7261022.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSIGWQGKNIAASGQTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4875..6518
                     /gene="ecpD"
                     /locus_tag="ACG0BW_004124"
     CDS             4875..6518
                     /gene="ecpD"
                     /locus_tag="ACG0BW_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFK7261023.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6487..7197
                     /gene="ecpE"
                     /locus_tag="ACG0BW_004125"
     CDS             6487..7197
                     /gene="ecpE"
                     /locus_tag="ACG0BW_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFK7261024.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7511..7840
                     /gene="ykgJ"
                     /locus_tag="ACG0BW_004126"
     CDS             7511..7840
                     /gene="ykgJ"
                     /locus_tag="ACG0BW_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFK7261025.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(8089..8703)
                     /gene="yagU"
                     /locus_tag="ACG0BW_004127"
     CDS             complement(8089..8703)
                     /gene="yagU"
                     /locus_tag="ACG0BW_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFK7261026.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2343 a         2223 c         2188 g         2006 t
ORIGIN      
        1 attacacaac acattaagac tattcctaac acctcagggc aaagttcctg gctaatataa
       61 aatgcaagta agaattgaac gttatattgc caataacctt atgaaactga atgtcttttt
      121 cttcttatca aaaaagcaat attttcattt tttgtaaata ttgacttaac catggaattc
      181 cttttctgtt cacatattga cactcatcag gaaaaaaaca taaatttaag cccaatcgaa
      241 aataattaaa acttaatctc gtttaaccta tattgatatg tgctacgtat cttatttact
      301 tccgatttac taaagaaact gaatgtacct gtaaaaatta caggtttgga aagtagtgac
      361 atggcaaaat gattacagca gggactatga ggttaaaaac catatggaat gtcaaaaccg
      421 ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac tatctgaact
      481 gattgtggat atcgacagat taatttatct atcgctggag aaaatcagaa aagatttcgt
      541 gtttatcaat ctcaatacgg attctttaac tgagtttata aaccgtgata atgagtggtt
      601 atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag aagccttagc
      661 aaattattgg tattacaaca gcaatattag gggcgtggta tacgctggac tgagtcgtga
      721 tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag atattaagaa
      781 agataaaatc actgaccggg aaatggaaat tatccgcatg acggctcagg gaatgctgcc
      841 taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc atcggcgtaa
      901 tgctgaggcc aagctgtact caaaaatata taagttggtt ccgtaaactc caggcaagtt
      961 agttttaaaa aatgactcac agggacatca cgtcctcaat tcaactcggg aagaaaagca
     1021 atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggttc aggtgtggcg
     1081 caggctgctg acgtaacagc tcaggctgta gcgacctggt cagcaacagc caaaaaagac
     1141 accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta tgccgaaggc
     1201 attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg ctatcgaggg tgactcaacg
     1261 gctaccgcct ttaaactgac ctcacgtctt atcaccaaca ccttaaccca gttggatacc
     1321 tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga aaaaactggc
     1381 gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag cccactggct
     1441 aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacttt ctccatcatc
     1501 agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc ggaaggcatc
     1561 tggagcggcg acgttagcgt acagttcgac gcgacctgga ccagttaatc tctctgatgt
     1621 accagcaggg gtagcccccc tgctttattc ctggacggaa ttgttatgaa aaagcacctt
     1681 ctgcctctcg ctctgctgtt ttccggaata tctccggccc aggcgctgga tgtcggcgat
     1741 atatcatcgt ttatgaacag tgacagcagc acgctgagca aaacgatcca aaacagtacc
     1801 gacagtggcc gcctcatcaa tatccgtctc gaacggctct cttcaccgct tgacgacggg
     1861 caggttatcg caatggacaa gccggatgag ttgctactca ctcccgccag cttgctgcta
     1921 cccgcccaag ccagcgaagt gatccgcttc ttctataagg gaccagcaga tgaaaaagag
     1981 cgctactacc gcattgtctg gtttgatcag gccctcagtg atgcgcagcg cgataatgcc
     2041 aaccgtagcg ctgtggccac tgcttccgcc cgcatcggca ccattctggt cgtcgccccc
     2101 cgccaggcga actaccactt tcagtacgcc aacggctccc tgacaaatac aggaaatgcg
     2161 acgctgcgga tcctcgccta cggcccctgc ctgaaagccg ccaatggtaa ggagtgtaaa
     2221 gagaattact acctgatgcc gggcaagtcg cgtcgtttta cccgcgtgga cacggcggat
     2281 aacaaaggac gggttgcact ttggcagggt gataagttca ttcccgtgaa atagatagct
     2341 gtgcagatgg ataatgacaa tgcctttacg acggttctcc ccaggactga aagcccagtt
     2401 tgccttcggc atggtctttt tgttcgttca gcccgatgcc agcgctgctg acataagtgc
     2461 gcagcaaata ggtggggtga ttattccgca ggccttcagt caggcgcttc aggacggcat
     2521 gagcgtccca ctttatattc atctcgccgg tagccagggg cgccaggacg atcagcgaat
     2581 cggcagcgct tttatctggc tggatgatgg acagctacgc atccggaaaa tacagctgga
     2641 agagagtgaa gataacgcca gtgtcagcga acaaactcga cagcagctga tggctctggc
     2701 gaacgccccg ttcaatgagg cccttaccat ccccctgact gacaacgcgc agctggatct
     2761 cagcttgcgc caactgctgc tgcagctggt ggtcaagcgc gaagcgctgg gcaccgtact
     2821 acgctcacgt agcgaagaca tcgggcagtc cagtgttaac accctcagca gtaatctgag
     2881 ctataacttc ggcgtctata acaaccagtt gcgtaacggc gggagcaaca catccagcta
     2941 tctgtcgctg aataacgtta ctgcactgcg cgaacatcac gtggtgctcg acggctcgct
     3001 gtacgggatc ggtagcggtc aacaggacag tgaattatat aaagcgatgt atgaacgcga
     3061 ttttgccggt caccgatttg ccggtggaat gctcgatacc tggaacttgc agtccttagg
     3121 gccgatgacc gccatttcag cagggaagat ttacggcctt tcctggggaa accaggccag
     3181 ctccaccatc ttcgacagca gccagtcagc cacgccagtg atcgcctttt taccggcggc
     3241 gggcgaagta catctcaccc gtgatgggcg gctactaagc gttcagaact tcaccatggg
     3301 caatcatgaa gtggataccc ggggtctacc gtacggtatt tacgatgtgg aagttgaggt
     3361 catcgttaac ggtcgcgtga tcagcaaacg cactcaacgg gtcaataagc tgtttagccg
     3421 ggggcgcggc gtcggtgcac cactggcgtg gcagatatgg ggcggtagct ttcatatgga
     3481 tcgctggtcg gaaaacggga aaaagacgcg accagctaaa gagagttggc tggcaggtgc
     3541 ctcgacctcc ggctcactga gtacgcttag ctgggcggca acgggatatg gatacgataa
     3601 tcaggcggtg ggtgaaaccc gtctgacgct gccgcttggg ggggcgatca acgttaacct
     3661 gcaaaacatg ctggccagtg acagctcatg gagcaacatc gccagcatca gcgccactct
     3721 acctggaggc ttcagttcgc tgtgggttaa ccaggaaaaa acccgcattg gcaatcaatt
     3781 gcgacgtagc gatgccgaca accgtgcaat cggcggcaca ctcaacctga actcactgtg
     3841 gtcgaagctg ggtacgttca gcatcagcta caatgatgac cgccgctaca acagccatta
     3901 ttacacggca gattactatc aaagtgtcta cagcggtacc tttggttcgc ttggcctgcg
     3961 ggccggtatt cagcgctata acaacggcga cagcagcgcc aatacaggga aatatatcgc
     4021 tctcgatctc tcgctaccac tgggcaactg gtttagcgca gggatgaccc atcaaaacgg
     4081 ctacaccatg gcaaacctgt cagcacgcaa acagtttgat gaaggaacca ttcgcactgt
     4141 tggtgccaat ctgtcacgag ccatctccgg cgataccggt gatgacaaaa ccctcagcgg
     4201 tggggcgtat gcacagttcg acgctcgtta cgccagcgga acgctgaacg tcaatagcgc
     4261 ggcggacggc tacatcaata ccaatttgac cgccaacggc agcatcggct ggcagggtaa
     4321 aaacatcgct gccagcgggc agaccgatgg caacgctggg gtgatattcg acactggcct
     4381 ggagaacgat ggtcagatca gcgccaaaat caacgggcgg attttcccgc ttaacggcaa
     4441 gcgtaactat ctcccgctct ctccctatgg gagatatgag gtggagttac agaacagcaa
     4501 aaactcactc gacagctacg atatcgtcag cggccgcaaa agtcatctga ctctctatcc
     4561 aggcaatgtc gctgtcattg agccagaagt gaagcagatg gttaccgtct ccggtcgtat
     4621 ccgtgcggaa gacggcacac tgctggctaa cgcacggatt aacaaccata tcggccgaac
     4681 tcgaaccgat gaaaacggcg agtttgtcat ggacgtggat aagaaatacc ccactatcga
     4741 ttttcgctac agtggcaata aaacctgcga agtggcgctg gaactcaacc aggcgcgcgg
     4801 tgccgtctgg gtcggtgatg tggtctgcag cggcctctca tcgtgggcgg cggtgacgca
     4861 gacaggagaa gagaatgaga gttaacctac tgatagcgat gataatcttt gcgctaatct
     4921 ggcctgcaac cgcgctcaga gcggcagtga gcaaaacaac ctgggcggat gctccggcac
     4981 gcgagtttgt gtttgtcgaa aacaactcag acgacaactt tttcgtcacg cctggcgggg
     5041 cgctggatcc gcgcctgacc ggtgccaacc gctggaccgg tttaaaatac aatggttcag
     5101 gaaccatcta tcagcaaagt ctcggctaca ttgataacgg ttacaacacc ggcctttata
     5161 ccaactggaa gtttgatatg tggctggaaa attcaccagt ttcatctcct ttaactggct
     5221 tgcgctgcat caactggtac gcagggtgta atatgaccac cagtcttatc ctgccgcaaa
     5281 ccaccgacgc cagtggattt tatggcgcga cggtgaccag cggcggcgcg aagtggatgc
     5341 acggcatgtt gtcagacgcg ttttaccagt atctgcaaca aatgcccgtc ggcagcagct
     5401 ttacaatgac catcaatgcc tgccagacct ctgtgaacta tgacgccagc agcggcgcac
     5461 gctgtaagga tcaggcctcc ggcaactggt atgttcgcaa cgtcacccat acgaaagcag
     5521 caaatctacg gttgataaat acccactcgc tggcggaggt gtttatcaac agcgacggag
     5581 taccgactct gggcgaaggg aacgccgact gccggacgca aaccatcggc agccgttcag
     5641 gattaagttg taagatggtt aactataccc tgcaaacaaa cggactcagc aacacctcaa
     5701 tccatatctt cccggcgatc gccaactcgt cgttagcctc ggccgtcggg gcgtacgata
     5761 tgcagttcag tctgaatggc agttcatgga aaccggtgag caatactgcc tattactaca
     5821 ccttcaacga gatgaagagc gcagactcga tctatgtttt cttctcgagc aacttcttta
     5881 agcagatggt gaacctgggg atcagcgata tcaacaccaa agatctgttc aactttcgct
     5941 ttcagaacac cacatcaccg gagtctggct ggtatgaatt ttctacctcc aacacgctga
     6001 ttatcaaacc ccgtgatttc agcatcagta ttatctccga tgaatatact cagacaccgt
     6061 cgcgggaggg atatgttggc agcggcgagt cggcactcga tttcggctat atcgtaacca
     6121 ccagcggtaa aacagctgcc gacgaagtgc tgatcaaggt gaccggaccc gcgcaggtga
     6181 ttggcgggcg ctcctattgt gtcttcagct ccgatgacgg taaggcgaaa gtaccgttcc
     6241 cggcgacact ttcctttatt acccgcaacg gagctacaaa aacctacgat gccgggtgcg
     6301 atgatagctg gcgggatatg accgatgcgc tgtggttgac cacaccgtgg actgatatct
     6361 ctggcgaagt ggggcagatg gataagacca cagtcaaatt ttcgattcca atggataacg
     6421 ccatttctct gcgtacggta gatgataacg gctggtttgg cgaagtcagc gcttcaggag
     6481 agattcatgt tcaggcgacg tggcgtaaca ttaactaagg ccctgctgac agcggtctgt
     6541 atgctggcgg cacctttgac acaggcgatt tcggtcggca atctgacatt ttcgctgccg
     6601 tccgagactg actttgtcag caaacgtgta gtgaataaca acaaaagcgc acggatatac
     6661 cgtattgcca tcagtgctat cgatagcccg ggcagcagtg aattgcgcac ccgaccggtg
     6721 gatggtgaac tgcttttcgc cccccgccag ctggcgttgc aggctggtga gagcgagtat
     6781 tttaaatttt actatcatgg tccacgggat aaccgcgagc gctactaccg ggtctcattt
     6841 cgcgaggtcc ccactcgcaa ccagacaaga cgcagcccaa ccggcggcga ggttagcacg
     6901 gagccggtgg tggtgatgga taccattctg gtagtacgac ctcgtcaggt tcagtttaaa
     6961 tggtccttcg acaaggtgac aggaacggta agtaacactg gcaacacatg gtttaagcta
     7021 ctgattaagc caggatgtga ttcgaccgaa gaggaaggcg atgcctggta tctacgtccg
     7081 ggagacgtgg ttcatcagcc tgagttacgt cagccgggga atcattatct ggtctataac
     7141 gacaaattca ttaagattag cgattcctgt ccggctaagc ccccttcggc ggactaagac
     7201 ttatccatcg gtcgagggaa gaattccacc tcagagactc aaataatcgt ttaaatgatg
     7261 tggaagcgat cgtcaatgtc acctgtaatg atgggctttc tcatatgtac accatagtgt
     7321 tcaggagaca cggaaaggtg ccaggcagcc tgctaacgag tggtttattc acgaattagt
     7381 acgtaaaatc ggtaacggct ggaaatcatt caatactcac actatcgaaa gttccccagc
     7441 caaccgcggt acgttcttac atacgatgta ccgctgttct ctttacgatt tatagctgta
     7501 ctggtgaatt atgagcaatc tgaatccatg catgacgtgt ggtgcctgtt gtgcattttt
     7561 ccgcgtctct ttttactggg ccgaagccga cgatgctggc ggtaccattc ccgccaggct
     7621 cactgaacaa atatcccctt ttcaccgatg catgagcggt accaatcaga aaaacccccg
     7681 atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc tgcacgatat ataaaaatcg
     7741 atcgtccaca tgcagagaat tcgccatgtc tggtgagaac ggagtcgtca atgaggcttg
     7801 caatcgtgca agagctaaat acgggctgac accgttataa atatgcaata attaattgca
     7861 ctgccccgcc agctataacc gcggggcttc gtttttcagg gataacaaaa cccgaaactt
     7921 cttccatctg ccagcaacaa tgcctcttcc tctatagtgt tctccggatc tacagcatcc
     7981 tgaagtagcg cgatgtactc actggctatc tgcttcagaa agtaaaaagc aatgttttta
     8041 acctataaaa atggcgctgc atttgcgcca tttttattat tcaatgcatt atctgtttga
     8101 gcctaaaggg atctcagggt ctggctcatg agtaattctg tttcgtaaat ctctgcgaat
     8161 aatttcaatg gaccagaacc agactaaatg tccaaaaatt tcagaaacat tctcatacca
     8221 cgggagatca aacagaggtg gcgtcagtcc catgagaggg aatgaaatca tatgaacaaa
     8281 aagttgggct aaagcacctg ccagtaagcc ctgccagagt ttaatttttg gaaatacttc
     8341 agcaaccaca caataaccga cagcaaacac tatcgaaaag ataatgtgcg taacgccaac
     8401 ccagttaaag acatgcccgg caaaggtata aacagccgca ttgggatctg tcagccctag
     8461 ccagtctcga agaaaaatat acggaggatt gagaaaatta cgggagcaat caatttgccc
     8521 agcagccctg attaatgatt ccgggccaca cgctgcatta aacatatcca ccgggctacg
     8581 tggcggcaat ggaacttcag caccccactt cacaaatgcg gaaacaacgc cagcaatcag
     8641 cccaatgaat gcagcaagac cataacgtct gcggttcggt ggagtttgtt caaatatatt
     8701 catatctacc ctgcttgtac cattatgtta tacacctctt caggagtatt cataaaacaa
//
LOCUS       DAYPHN010000179         8587 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000179 DAYPHN010000000
VERSION     DAYPHN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.179"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(259..522)
                     /gene="rpsT"
                     /locus_tag="ACG0BW_004128"
     CDS             complement(259..522)
                     /gene="rpsT"
                     /locus_tag="ACG0BW_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFK7261027.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            625..843
                     /locus_tag="ACG0BW_004129"
     CDS             625..843
                     /locus_tag="ACG0BW_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFK7261028.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            851..1792
                     /gene="ribF"
                     /locus_tag="ACG0BW_004130"
     CDS             851..1792
                     /gene="ribF"
                     /locus_tag="ACG0BW_004130"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFK7261029.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1835..4651
                     /gene="ileS"
                     /locus_tag="ACG0BW_004131"
     CDS             1835..4651
                     /gene="ileS"
                     /locus_tag="ACG0BW_004131"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFK7261030.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKELLGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLFAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4651..5145
                     /gene="lspA"
                     /locus_tag="ACG0BW_004132"
     CDS             4651..5145
                     /gene="lspA"
                     /locus_tag="ACG0BW_004132"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFK7261031.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5232..5681
                     /gene="fkpB"
                     /locus_tag="ACG0BW_004133"
     CDS             5232..5681
                     /gene="fkpB"
                     /locus_tag="ACG0BW_004133"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFK7261032.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5683..6633
                     /gene="ispH"
                     /locus_tag="ACG0BW_004134"
                     /gene_synonym="lytB"
     CDS             5683..6633
                     /gene="ispH"
                     /locus_tag="ACG0BW_004134"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFK7261033.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6699..7613
                     /gene="rihC"
                     /locus_tag="ACG0BW_004135"
     CDS             6699..7613
                     /gene="rihC"
                     /locus_tag="ACG0BW_004135"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFK7261034.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7780..>8587
                     /gene="dapB"
                     /locus_tag="ACG0BW_004136"
     CDS             7780..>8587
                     /gene="dapB"
                     /locus_tag="ACG0BW_004136"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFK7261035.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLD"
BASE COUNT         1949 a         2162 c         2449 g         2027 t
ORIGIN      
        1 atgtgtatca caatctccac attctgtgta gggggagtct gaagattgag tagatattct
       61 tattcagaat gtatcaaccg atgattctac gatttttaag tcacgaagag ttcagagagt
      121 gtaactgcat gtctgcaaga accagcatct tttgactatc tggcaaccgg cagtgtgttt
      181 tctaacacat cacaaagcag caggtataaa aaaacccgct tgcgcgggct ttttcacaaa
      241 gcttcagcaa attggcgatt aagccagctt gttgatctgt gcagtcaggt tagccttatg
      301 acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca cgatcggttg
      361 catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac
      421 tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac gagccttttc
      481 agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac tcccaaatgt
      541 gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct tttgtcaatg
      601 gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga tggcgcagga
      661 ttctaccagc ttgcggggtg tgaatacagc ttttccgcga taaaaattgc agcaggtggt
      721 cagtttcttc ccgtgatttg cgccatggca atgaaaagcc acttctttct gatttcggta
      781 ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt ttcactgttt
      841 tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc cgcaagaagg
      901 gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca
      961 gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc tttttgaacc
     1021 tcaaccactg gaactgtttg caaccgataa agccccggca cgactgaccc ggctgcggga
     1081 aaaactacgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtac gtttcgacag
     1141 gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat cttctggtga agcatttgcg
     1201 cgtaaaattt cttgctgtag gggatgattt ccgctttggc gctggtcgtg aaggcgattt
     1261 cttgttatta cagaaagctg gcatggaata cggcttcgat atcaccagta cgcaaacttt
     1321 ttgcgaaggt ggcgtgcgta tcagcagcac cgccgtgcgt caggcccttg cggatgacaa
     1381 tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc gtgtagtcca
     1441 cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc tgcgccgtca
     1501 ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg aaaagccgtt
     1561 acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc agcagctgga
     1621 agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag tagtgctgcg
     1681 taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag cgcagattgc
     1741 gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt aagcctgtta
     1801 tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat caaccctgaa
     1861 tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac cgggaatgct
     1921 ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa aaggcaaaaa
     1981 aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata ttggtcactc
     2041 ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg gttatgactc
     2101 gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag tagagcaaga
     2161 atacggtaag ccgggtgaga aattcaccgc cgctgagttc cgcgccaagt gccgcgaata
     2221 cgctgcgacc caggttgacg gtcagcgcaa agactttatc cgtctgggcg tgctgggtga
     2281 ctggtcccac ccgtacctga ccatggattt caaaactgaa gccaatatca tccgcgcgct
     2341 gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg aagccggtac actggtgcgt
     2401 tgactgccgt tctgcgctgg cggaagcgga agttgagtat tacgacaaaa cttctccgtc
     2461 catcgacgtt gctttccagg cggtcgatca ggatgcactg aaagcaaaat ttgccgtaag
     2521 caacgttaac ggcccaatct cgctggtgat ctggaccacc acgccgtgga ctctgcctgc
     2581 gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga tcgacggtca
     2641 ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg gcgtgaccga
     2701 ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag ctgctgcgct ttacccatcc
     2761 gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctgg atgccggtac
     2821 cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg gtcagaaata
     2881 cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg gtacttaccc
     2941 gacgctggat ggcgtgaacg ttttcaaagc gaacgacatc gttgttgcgc tgctgcagga
     3001 aaaaggcgcg ctgttgcacg ttgagaaaat gcagcacagc tatccgtgct gctggcgtca
     3061 caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc gtcagcatgg atcagaaagg
     3121 tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggatcccgg actggggcca
     3181 ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc gtcagcgcac
     3241 ctggggcgta ccgatgtcac tgttcgtgca taaagacacg gaagagctgc atccgcgtac
     3301 cctcgaactg atggaagaag tggctaaacg cgttgaagtt gacggcatcc aggcgtggtg
     3361 ggatctcgat gcgaaagagc tcctcggcga cgaagctgat cagtacgtga aagtgccgga
     3421 tacgctggat gtatggtttg actccggatc tacccactct tccgttgttg acgtgcgtcc
     3481 ggaatttgcc ggtcacgctg cggacatgta tctggaaggt tcagaccagc accgtggctg
     3541 gttcatgtcc tctctgatga tctctaccgc gatgaagggc aaagcaccgt atcgtcaggt
     3601 actgacccac ggctttaccg tggatggtca gggccgcaag atgtctaaat ccatcggcaa
     3661 caccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc gtctgtgggt
     3721 ggcatcaact gactacaccg gtgaaatggc cgtttctgac gagatcctga aacgtgctgc
     3781 cgacagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc tgaacggttt
     3841 tgatccggca aaagatatgg tgaaaccgga agagatggtg gtactggatc gctgggccgt
     3901 aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat acgatttcca
     3961 cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct tctacctcga
     4021 catcatcaaa gaccgtcagt acaccgccaa agcggacagt gtggcgcgtc gtagctgcca
     4081 gacagcgctg tatcacatcg cggaagcgct ggtgcgctgg atggcaccaa tcctctcctt
     4141 caccgctgat gaagtgtggg gctacctgcc gggtgaacgt gaaaaatacg tcttcaccgg
     4201 cgagtggtac gaaggcctgt ttggtctggc agacagtgaa gcgatgaacg atgcgttctg
     4261 ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag cgcgtgccga
     4321 caagaaagtg ggtggctcgc tggaagcggc agtgaccttg ttcgcagaac cggaactggc
     4381 ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct ccggcgctac
     4441 cgttgcagac tataacgacg cacctgctga tgcccagcag agcgaagtac tcaaagggct
     4501 gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc actacaccca
     4561 ggatgtcggc aaggtagcgg aacacgcaga aatctgcggc cgctgtgtca gcaacgtcgc
     4621 cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc aacagggcta
     4681 cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa gtacctgatc
     4741 ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa tctgcattat
     4801 gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg gcagcgttgg
     4861 ttctttgccg gtattgcgat tggtattagc gtgatcctgg cagtgatgat gtatcgctcg
     4921 aaggccacgc agaagctaaa caatatcgct tacgcgctga ttattggcgg cgcgctgggc
     4981 aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt ctacgtcggc
     5041 gactggcact tcgccacctt caaccttgcc gatactgcca tttgtgtcgg tgcggcactg
     5101 atcgtgctgg aagggttttt accttctaaa gcgaaaaaac aataataaac cctgccggat
     5161 gcggcctaca gattgctacg aaatcgtagg ccgcatccgg caaaaatcct caaaaataag
     5221 agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg cacttcacgc
     5281 taaaactcga cgatggcacc accgccgagt ctacccgcaa caacggtaaa ccggcgctat
     5341 tccgcctggg cgatgcttct ctttccgaag ggctggagca acacctgctg gggctgaaag
     5401 tgggcgataa aaccactttc tcgctggagc cagatgcggc gtttggcgtg ccgtcaccgg
     5461 atctgattca gtacttctcc cgccgtgagt ttatggatgc aggcgagcca gaaattggcg
     5521 caatcatgct ttttaccgca atggatggca gtgagatgcc tggcgtgatc cgcgaaatta
     5581 acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc gttcattttg
     5641 atattgaagt gctggaaatc gatccggcac tggaggcgta acatgcagat cctgttggcc
     5701 aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga aaacgcgctg
     5761 gccatttacg gcgcaccgat atatgtccgt cacgaagtgg tgcataatcg ctacgtggtc
     5821 gatagcctgc gcgagcgtgg ggctatcttt attgagcaga ttagcgaagt gccggacggc
     5881 gcgatcctga ttttctccgc acacggtgtt tctcaggcgg tacgtaacga agcgaaaagc
     5941 cgtgatttaa cggtattcga cgccacctgc ccgctggtga ccaaagtgca tatggaagtc
     6001 gcccgcgcca gtcgccgtgg cgaagaatct attctcatcg gccacgccgg gcacccggaa
     6061 gtggaaggta cgatgggcca gtacagtaac ccggaagggg gaatgtatct ggtcgaatcg
     6121 ccagacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt tatgacccaa
     6181 accactctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa acgcttcccg
     6241 aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg tcaggaagcg
     6301 gtacgcgccc tggcagaaca ggcggaagtt gtgctggtgg tcggttcgaa aaactcctcc
     6361 aactccaacc gtctggcgga gctggcccag cggatgggca aacgcgcgtt tttgattgac
     6421 gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg cgtgactgcg
     6481 ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg cacgtttgca gcagctgggt
     6541 ggtggtgaag ccattccgct ggaaggccgt gaagaaaaca ttgttttcga agtgccgaaa
     6601 gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag ttatgcgaaa
     6661 atgccggtct tgttaccggc attttttatg gagaaaacat gcgtttacct atcttcctcg
     6721 atactgaccc cggcattgac gatgccgtcg ccattgctgc cgcgattttt gcccccgaac
     6781 tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa acgacccgca
     6841 acgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccag ggagccgctg
     6901 tgccgctggt acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg ggaatggctg
     6961 gctacgactt tgttgagcac aaccgaaagc cactcgggat accagcgttt ctggcgattc
     7021 gtgatgccct gatgcgtgca ccagagcctg ttaccctggt ggccattggc ccgttaacca
     7081 atattgcgct gttactttca caatgcccgg aatgtaaacc gtatattcgt cgtctggtga
     7141 tcatgggcgg ttctgctggt cgcggcaact gtacaccaaa tgccgagttt aatatcgccg
     7201 ccgatccaga agcggctgcc tgtgtcttcc gtagcggcat cgaaatcgtc atgtgcggtc
     7261 tggatgtcac caatcaggca atattaaccc ctgactatct cgctactttg ccggagttga
     7321 atcgcaccgg aaaaatgctc cacgccctgt ttagccacta ccgtagcggc agtatgcaaa
     7381 gcggtctgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg gacttattca
     7441 ccctcaagcc gtgttttgtc gcggtagaaa ctcaaggcga atttacctca ggcacgacgg
     7501 tggttgatat cgacggttgc ctgggcaaac cagccaatgt acaggtggca ttggatctga
     7561 atgtaaaagg cttccagcag tgggtagctg aggtgctggc tctggtgccg taacctgtca
     7621 catgttatcg gcatgcagtc attcatcgac tcatgccttt cactgatatc cctccctgtt
     7681 tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc gctggttact
     7741 ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc aaacatccgc
     7801 gttgccatcg cgggagccgg gggccgtatg ggccgccagt tgattcaggc ggcgctggca
     7861 ttagagggcg tgcagcttgg cgcggcgctg gagcgcgaag gatcttcttt actgggcagc
     7921 gacgccggtg agctggctgg agcagggaaa acaggcgtta ccgtgcaaag cagcctcgat
     7981 gcggtaaaag atgattttga tgtgtttatc gattttaccc gtccggaagg tacgctgaat
     8041 catctcgctt tttgtcgcca gcatggcaaa gggatggtga tcggcactac ggggtttgac
     8101 gaagccggta aacaggcgat tcgtgacgca gccgccgata ttgcgattgt ctttgcagcc
     8161 aattttagcg ttggcgttaa cgtcatgctt aaactgctgg agaaagcagc caaagtgatg
     8221 ggggactaca ccgatatcga aattattgaa gcacatcata gacataaagt tgatgcgccg
     8281 tcaggcaccg cactggcaat gggagaggcg atcgcccacg ctcttgataa agatctgaaa
     8341 gattgcgcgg tctacagtcg tgaaggccat accggtgaac gtgtgcctgg caccattggt
     8401 tttgccaccg tgcgtgcagg tgacatcgtt ggtgaacata ccgcgatgtt tgccgatatt
     8461 ggcgagcgtc tggagatcac ccataaggca tccagccgca tgacatttgc taacggtgcg
     8521 gtaagatcgg ctttgtggtt gagtggtaag gaaagtggtc tttttgatat gcgagatgtg
     8581 cttgatc
//
LOCUS       DAYPHN010000180         8586 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000180 DAYPHN010000000
VERSION     DAYPHN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.180"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..754
                     /locus_tag="ACG0BW_004137"
     CDS             <1..754
                     /locus_tag="ACG0BW_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050135 - NADP+ nucleosidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFK7261036.1"
                     /translation="SINNNSDLELFNADYRKWSDFNAELLKRSFTNDDFKEEYEGGAF
                     GVISLYETSLGEKISEATRKLQSKIRKLESIMERLELIPISSKVVEENIEVPQSSAVK
                     TKKVFIVHGRDEISKTNLEVFLREIGLEPIVLHRQADVGQTVIEKFEANSDVGFAFIL
                     LTPDEIAYLLPQDSLPDNEREKELRARPNVMFEFGYFVGKLGRSKVCCLYTGDVVIPS
                     DLNGLIYKKFNSSIEEVAYSIIKDLRATGYAV"
     gene            complement(830..1009)
                     /locus_tag="ACG0BW_004138"
     CDS             complement(830..1009)
                     /locus_tag="ACG0BW_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261037.1"
                     /translation="MSTHKNQRRGNPPFQFRLDPELRELMEQAQQVDGDESLAAWIKR
                     IIRKELQQRGYEVKI"
     gene            1140..1505
                     /locus_tag="ACG0BW_004139"
     CDS             1140..1505
                     /locus_tag="ACG0BW_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFK7261038.1"
                     /translation="MAGTQHTQTHPKFIYTFLALHHDRMADGATTVHVAADTLVDARK
                     MVKEMGYTAAFWKGREENTLFIQKCENNFIWRFIALSTAQPRVIHIVATSEQEARQQS
                     PAGCVMVFAARIRQGVCHA"
     gene            1498..1818
                     /locus_tag="ACG0BW_004140"
     CDS             1498..1818
                     /locus_tag="ACG0BW_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261039.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            1825..2124
                     /locus_tag="ACG0BW_004141"
     CDS             1825..2124
                     /locus_tag="ACG0BW_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFK7261040.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            2121..3938
                     /locus_tag="ACG0BW_004142"
     CDS             2121..3938
                     /locus_tag="ACG0BW_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFK7261041.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            4226..4471
                     /locus_tag="ACG0BW_004143"
     CDS             4226..4471
                     /locus_tag="ACG0BW_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261042.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            4468..4890
                     /locus_tag="ACG0BW_004144"
     CDS             4468..4890
                     /locus_tag="ACG0BW_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFK7261043.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     APMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQSGNDDPYGDNIPF"
     gene            5108..6148
                     /locus_tag="ACG0BW_004145"
     CDS             5108..6148
                     /locus_tag="ACG0BW_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFK7261044.1"
                     /translation="MVDLYSPTQLVQVANAEDVQKKLNALFTSLFFTRSVMFESRDII
                     LDTIDDPNIPIAAFCSPMVGSKVSRDEGYESKTIRPGYMKPKSSIDPNKLAVRPAGVS
                     PEQYNAFGARNIKVKQAIVNQAKAIRTRIEWLAVQAITTGKNIIEGDGIERYELDWNI
                     KPQNIITQSGGTEWSGKDKETFDPNDDIESYAEFSEGVTNIIIMGGNVWKKYRSFRAI
                     KEALDTRRGSNSELETALKDLGDSVSFKGYMGDVAIVVYSGRYTDEDGTEKHFLDPDL
                     MVLGNTALQGIVAYGGIQDPELIRMGLTKAELAPKNYIVPGDPAIEYVQTHSAPQPIP
                     ARINRFVTVRIG"
     gene            6159..6500
                     /locus_tag="ACG0BW_004146"
     CDS             6159..6500
                     /locus_tag="ACG0BW_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFK7261045.1"
                     /translation="MATHYTELMAGTEALVTTLGIFSANKGVIPAFTPLMQEDTTGAL
                     VVWDGTSAGKAVYVSAVQLDTAKKTQAQVYKTGVLNVDALNWPESVKELSVKVAAFVG
                     SGISVQPLARV"
     gene            6512..6895
                     /locus_tag="ACG0BW_004147"
     CDS             6512..6895
                     /locus_tag="ACG0BW_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7261046.1"
                     /translation="MQNHYNDLKPIAEMMYPDPAVEELKAIADKMRLSERLVDMNQVM
                     ELTTLSRRTLLNLEARGEFPERVQVTEGRKAWYLSEVIDWINNIPRASEYCRVPVPKK
                     PDAALCLKIDRVRRNARDGRYKLIG"
     gene            7097..7639
                     /locus_tag="ACG0BW_004148"
     CDS             7097..7639
                     /locus_tag="ACG0BW_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFK7261047.1"
                     /translation="MKLHLMNKKNMAKSCRVSATAFDKWGVIPVERKGREAFYDVASV
                     IDNRVNNAISQITNETGDIDDDELLRVRIRLLTAQAEAQELKNERERGDVIDTEFCMY
                     VLSKLASQISSIMDSLPLTMQRRFPQMTPAMLDGLKKEVAKACNASAGVADNLPQILA
                     DYLMESTGNVPDKLQLNKDK"
     gene            7651..7926
                     /locus_tag="ACG0BW_004149"
     CDS             7651..7926
                     /locus_tag="ACG0BW_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261048.1"
                     /translation="MTESELLKVICHAGGVSHQHDEQTTQPGSVTAENYARVVAEVMW
                     RDGIELNGQDCLVIRTKVLAILAARRRQGQRQNVASYQWKKPDRLRR"
     gene            complement(8329..>8586)
                     /locus_tag="ACG0BW_004150"
     CDS             complement(8329..>8586)
                     /locus_tag="ACG0BW_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFK7261049.1"
                     /translation="LYKTLSKELNLSADQHTENIFKQILGGCSAVVNGLGTLRNKLGD
                     AHGTNSSSIKPSPRHAELAVNLSGSMALFLINTWLNLKDGK"
BASE COUNT         2497 a         1709 c         2208 g         2172 t
ORIGIN      
        1 aagcataaat aacaatagtg acttggagct gtttaatgct gattacagga aatggagtga
       61 ttttaatgct gagctactga agcgtagttt cacaaatgat gatttcaaag aagaatatga
      121 aggtggggca tttggtgtta tttcattgta cgagacttcc ttgggagaaa aaatcagtga
      181 ggctacgcgt aaactccaga gtaagataag aaaactagag tcgattatgg agcgactgga
      241 gttaattcct atatcttcca aagttgttga agaaaatatt gaagtacctc agagttcagc
      301 ggtaaaaacc aaaaaggtat tcattgttca tggaagagat gaaatatcaa aaaccaactt
      361 agaagtgttt ttaagagaaa ttggcttaga acctattgtt ctccataggc aagctgatgt
      421 aggtcagaca gtgattgaga aatttgaagc aaatagtgat gttggtttcg cattcattct
      481 tctcacacct gatgagatcg cttatttatt gccgcaagac tctttacctg ataatgaaag
      541 agaaaaggag ttacgtgcac gaccaaatgt aatgtttgaa tttggttatt tcgtagggaa
      601 attaggtcgt tctaaggttt gttgtctata cacaggtgat gtagtgattc caagtgattt
      661 gaatggtctt atttataaaa aattcaattc ttctattgaa gaagtagcat atagcattat
      721 taaagacttg cgagctactg gttacgcagt ttagtaaatt ctttgaacta aaaatctgca
      781 tagatataga gccattcgag ataaatggct ctatatgaaa tattatctgt taaattttga
      841 cttcatatcc gcgttgctgt agttctttgc gaataatcct tttaatccag gctgctaaag
      901 attcgtctcc atctacttgc tgtgcttgct ccatcaactc cctcaactca ggatctaacc
      961 gaaactggaa cggaggatta ccacgtcttt gatttttgtg tgttgacacg tcaattacac
     1021 ccgatgtaat gtgttaatgt gtgatgacac attacatacg tgtttaggaa aatgcaacgc
     1081 ctcgacgtgc gggaacacca tcgaggcgtc taaccaaccc gttaaatgag gtaacgatta
     1141 tggctggaac acagcatacc caaactcacc ctaaatttat atacaccttt ctggcactac
     1201 accacgaccg catggcagat ggagcaacta cggtacatgt agccgctgac acgctggttg
     1261 atgcccgcaa gatggttaag gagatgggct atactgcggc tttctggaaa gggcgtgaag
     1321 aaaacacgct gtttattcag aaatgcgaaa acaatttcat ctggcgtttt atcgccctga
     1381 gcaccgcaca accgcgcgtg attcacatcg tggccaccag cgaacaggaa gcacgccagc
     1441 aatccccggc tggctgcgtg atggtattcg ccgcccgtat tcgtcagggg gtatgccatg
     1501 cctgatatgt caaattacca gtacctgatt aatccgcatt ttaactgtga gcatgatatt
     1561 gctaaaaagg tttattccgc tgcggatggg gctactgaca atatatcaat ggctgttgcg
     1621 tcaattggta gcctgatgtg gcatgcgtca gaaaatgagg actatgacga aaaggctatg
     1681 cgcattgata tgggtaatat cggtttgtta ctggcaatgc ttgggcagtt tgatatttcg
     1741 ttacggtgca ccattgaaaa tgccacagat gcattaaatg ccataaagaa agcgaatact
     1801 gattcaaatc ggggataaat aatcatgaga acatatttat ctggcttgac tgccagcggt
     1861 tatgcacacc ccaaaattat ccccggcgct atttatctgg ataagaacgg taacagagta
     1921 acggtaaaag aactgatgtt tgaccgtgtg tattttattc gtgatggcta ttcatttcat
     1981 agttcgctga acgtggagat ctttattagc agattcaggc gggaaatccc gctttccaga
     2041 aataaccatg tgtcatgtat ggatgttgat aaaaaactac aggaactgaa aaacatgatt
     2101 gccgcgtgga gagagcagaa atgaaaaaag cgccaaattt aaaacaccag ccgcgtgaca
     2161 aaatgacgga agtcatcatt tttgcgggta gtgatgcgtg ggcacatgcg aaacagtggc
     2221 aggaacagga cgggcgactg gctggcgata acgtgccacc tgtctggctt ggagagcaac
     2281 aacttgccga actggacaac ctgcaaatcg taccggacgg acgctatcgc gtgcgtctct
     2341 atcaggcggg gttattgcgt ccggggcttg ttaataccat cgggcagaaa ctggcagtgg
     2401 caggtgtcag ggatgctgat tattaccctg aaggaatgca cagccagaaa cgggagaact
     2461 ggcgcgaata tctggaacgt gaacgggcag agcaggcgga aaagaaaaag gtagttgaac
     2521 tgcctgtaaa gaaaaaagag cgggtaaaag acgataacgc ttcatcactg gcgcttaacc
     2581 agatgggagc aagtcaacgc ggcgaagttc tcctggcaca ttatggcggt gaactggcga
     2641 ttcatgctga ctctgacact gttcaccatt acaacggcgt tgtatgggag ccagtacagg
     2701 ataaagaatt acagcgagct atggcacaga ttttcattga tgcggagatc agctattcgc
     2761 agaacgccat taaatcggcg gtcgatacca tgaagttaag tttgcctgta atggggaata
     2821 cagcccgtaa cctgattgga ttcagtaacg gggtatttga taccagaaca ggtaattttc
     2881 gggagcataa caaaaacgac tggttgttaa ttgccagtga attacctttc agcccaccag
     2941 cagaggggga aacgctggca acacatgcgc cgaatttctg gaagtggtta cgccgttcgg
     3001 tggctgagaa tgaccgcaag gcggatcgcg tactggctgc attattcatg gtgctggcga
     3061 accggtacga ctggcagtta ttcattgagg taacaggtcc agggggaagt ggtaaaagcg
     3121 tgatggcgga gatttgcacc atgctggcgg gtaaggccaa cacagtatcg gcaagcatga
     3181 aggcgctgga agatgcaagg gaacgcgcgt tagtggttgg cttttcgctg attatcatgc
     3241 cggatatgac ccgctacgct ggtgatgggg cagggattaa ggctattaca ggcggtgaca
     3301 aggtggcaat tgacccgaaa cacaaagccc cctactcaac gcgtattccg gcagtagtgc
     3361 tggcggttaa caataacgcc atgtcattca gtgaccgcag cggggggatc tcacgtcgtc
     3421 gggtgatatt caatttttcg gaagttgtac cggagaacga acgcgatcca atgctggcgg
     3481 aaaaaataga aggtgagttg gcggtagtga ttcgccatct gcttacacgg tttgctgacc
     3541 aggacgaagc cagacgcctg ttatatgagc agcagaaatc tgaagaagca ctggcgataa
     3601 agcgagaggg ggattcgctg gtggacttct gcggctatct catggcgtcg gtaatgtgtg
     3661 atggcctgtt agtgggtaat gctgaaattg tgccattcag cccacgcagg tatctctatc
     3721 atgcctatct ggcttatatg agggcacatg ggtttggtaa acctgtaaca ctgacgcgct
     3781 tcggtaaaga tatgccgggg gcaatggcgg aatatggcag ggagtatatg aaacggaaaa
     3841 cgaagcacgg tttgcgttca aacgtgacac tgacggagga atcagaagac tggatgccat
     3901 catgtgtatc ggtcactaat gacgatagca aaaattaaac ttatggaata actgttcacc
     3961 actgttcacc ctgtcataaa tatcttttat atcagtatat tatagggtga acagttattt
     4021 atgaactgtt caccaaacta ttcactgttc acctttttga ttgtttattg agcttcaagg
     4081 gtgaacagtg gtgaacagtt ggtgaatagt ttttgtgaaa ctgttcaccc cttaacatta
     4141 tgaattaaaa gagaaaatat caaaaggtga acaggtgaag ggttaaaacg caaaaatttt
     4201 aatttactgc tgtgagataa agcctatgac agcgaagcac acaaaaaaat cacaatcgca
     4261 cgcccttgat ttgacggaac actggttaag ggtgtcgata aaaatcattg accgcaacgc
     4321 cggggaagga tatgcgaaag cacatcccga actgattagc gcattcatga caacggcagc
     4381 tgcaaacttt gccacgctga cagaacggga gattgccgaa gcggaacagg tgacaaccat
     4441 caacgttaaa accggagagc agacagcatg acagcacaga tagcggctta cggacggctg
     4501 gtggctgacc cgcagttaaa gaccaccagc aagggtacac aaatgacgat ggcgagtatg
     4561 gcggtccccc ttccgtgcag ccaggcagat gacggaacgg cgccgatgtg gttatccgtc
     4621 ctggcgtttg gcagacaggc cgacgcactg gcaaaacacc acaaaggcga actggtgagc
     4681 gtggcgggta acatgcaggt aagccagtgg acaggccaga acggcgaaac gcggcagggc
     4741 tggcaggtta tcgcagacag tgtaatcagt gccagaacgg cgcgaccggg cggcaaaaaa
     4801 ggtcaacagg gccaggctac tgacgcactg aacagggcaa aacaacagtc ggggaatgat
     4861 gatccgtacg gcgataatat accgttttaa attctgcaaa taaaaaaatg ccggaagaaa
     4921 atagattttc cggcatgcta cataaatccc gaccaaagga agtaaataca ttaacacgaa
     4981 ttatcagcac tgaagttgtc acggcatatt ttatacaaca ttgcacttgg ttgcatgtat
     5041 tcgcatagca gacatcggta atagaatata ttcacaatta tttgtaatga atgtaaagag
     5101 gatgagtatg gttgatttat attcgcctac acagcttgtg caggtggcta atgctgaaga
     5161 tgtgcaaaaa aaattaaatg cgttgtttac cagtttgttt ttcactcgct cggtaatgtt
     5221 tgaatcgaga gacattattc ttgatacgat cgacgatcca aatatcccga tcgcggcgtt
     5281 ttgctctcct atggtgggca gtaaagtttc acgagatgag ggatacgaat caaaaacaat
     5341 tcgccctggc tatatgaaac cgaaaagcag cattgatcca aataagttag ctgtgcgccc
     5401 tgctggtgtg tcacctgagc aatacaatgc ttttggggcg cgtaatatta aagttaaaca
     5461 ggcgattgta aatcaggcta aagctattcg tacacgtatt gaatggcttg ctgttcaggc
     5521 aatcacaacg gggaaaaata tcattgaggg cgatggtatt gaacgttatg agctggactg
     5581 gaatataaaa ccacaaaata tcatcactca gtctggcggt actgagtggt caggtaagga
     5641 taaagaaact tttgatccaa atgatgatat tgagagctac gcagaattta gtgagggcgt
     5701 cactaatatc atcattatgg gcggtaatgt atggaagaaa taccgttcat tcagagcgat
     5761 aaaagaggct ctggataccc gtcgtggttc taattccgaa ctggaaacgg cccttaaaga
     5821 ccttggtgat tcggtgagtt ttaaagggta tatgggcgat gttgcgattg ttgtttacag
     5881 cgggcgttat accgacgagg acggaactga aaaacatttc cttgatcctg atttgatggt
     5941 gcttggcaat acggctcttc aggggattgt cgcttatggc ggtattcagg atccggagct
     6001 aatccggatg gggctgacta aagccgaact tgcaccgaaa aactatattg tgcctggtga
     6061 tccggctatt gaatatgtgc agacacattc agcaccacag ccaataccgg cccgtatcaa
     6121 tcgttttgtt accgttcgca ttggctaagg ggggagcaat ggctactcat tacactgaac
     6181 tcatggctgg cactgaagca ctggttacta cgctggggat attttcagct aataaagggg
     6241 taattcctgc atttacgcca ctgatgcagg aagatacaac aggtgcactg gtggtatggg
     6301 atggtacgag tgcaggtaaa gctgtttatg tttccgctgt acaactcgat acagcgaaaa
     6361 aaacacaggc tcaggtctat aagacaggtg tcttaaatgt tgatgctctt aactggcctg
     6421 agtcagtaaa agaactgtca gtaaaggttg cagcgtttgt tggctcaggt atttctgttc
     6481 agccgctggc tcgtgtgtaa agggggatac aatgcagaat cattacaatg accttaagcc
     6541 aattgccgaa atgatgtatc cggatccagc agtagaggaa ttaaaggcta ttgctgacaa
     6601 aatgcgttta agcgagcgtc ttgttgatat gaatcaggtg atggaactta caaccctgag
     6661 tcgtcgtaca ctgctaaacc ttgaagcacg tggagagttc cccgaacgcg tgcaggttac
     6721 ggaagggcgt aaggcctggt atttaagcga agtgatcgac tggataaaca atattccgcg
     6781 cgcttctgaa tattgccgcg tacctgtccc aaaaaagcca gatgcggcgc tatgcctcaa
     6841 gattgatcgt gtacgccgca atgcacggga tggtcgctat aagctgattg gttgatgaaa
     6901 ttagggcccg ttctggctgg cgggtccttt ccggcgatcc ggcaggttac ggggcgtcag
     6961 gcgcgcgggt tttcgctatt tatgagcatt ttaaggggac tggtggtggt tttgttgttc
     7021 gctatatctg tatgaataat aagagaaaaa tacaatcaat acaccaacct gaaacagtaa
     7081 ttaagttgtg gtatcaatga aattacacct gatgaacaaa aaaaacatgg caaaaagttg
     7141 ccgtgtaagc gccactgcgt tcgataagtg gggagtgatt cccgtcgagc gtaagggccg
     7201 tgaggcgttc tatgatgttg ccagtgtaat agataatcgg gttaacaatg caatcagcca
     7261 gattacaaac gaaacgggcg atattgatga cgatgaactt ttacgcgtca ggatcagatt
     7321 actgacagca caggcagagg cgcaggagct taaaaacgag cgcgaacgcg gcgacgttat
     7381 tgatacagag ttttgtatgt atgttctttc caaactggcg agtcagattt catctatcat
     7441 ggacagcctg ccgcttacca tgcaaaggcg cttcccacag atgactccgg ctatgctgga
     7501 tggacttaaa aaggaagtgg ctaaagcctg taatgccagt gccggcgttg ctgacaacct
     7561 cccacagata ctggctgatt atctgatgga aagtacagga aacgtaccgg ataagttgca
     7621 gctgaataag gataagtaac gtagtacgct atgactgaat ccgaactact gaaagtaatc
     7681 tgccatgctg gtggagtcag ccaccagcat gacgaacaga ccacgcagcc gggcagtgtc
     7741 accgctgaaa attacgctcg tgtggttgct gaggttatgt ggcgtgacgg tatagaactg
     7801 aacggacagg attgcttagt catccgcacc aaagtgctgg ctatactggc agctaggcgg
     7861 aggcagggac aacgccagaa cgttgcatcg taccagtgga agaagcctga cagactgcgg
     7921 cgataactct ttgattttct cgacggcccc aaaatgggcc ttaccatagc cagctaataa
     7981 atgagcaagc ctcaaaagtg aggtttgtgg ccgtttggcc tattcatatg ctaactcgtt
     8041 gatattcctg acgacgcaaa tttacgcctt gtggctggta gtcgaattgc aaatttgcaa
     8101 ctcaactatg aaactacagg tagtttgggt agtaaaagca acacactgat tttggggctt
     8161 cttcgcgata cctaatatta tggtatcggt ggaagagata tcgtttctca taggttagca
     8221 ccgagggcgg agttccgccc tcatcgaaaa attgcttacg agtcgtcaaa ataaattaat
     8281 cgactgagta taatgattaa ctaattgctc gtgttacttc attatacgtt atttcccatc
     8341 tttaaggttt aaccaagtgt taattaaaaa aagagccatt gagcctgata aatttactgc
     8401 aagttcggca tgccttgggg atggcttaat tgatgagctg ttcgttccat gtgcatcacc
     8461 gagtttatta cgaagagtac ctaacccatt tacaactgca gaacaaccgc cgagaatttg
     8521 tttgaagata ttttcagtat gttggtcggc agataggtta agttcttttg aaagagtttt
     8581 ataaag
//
LOCUS       DAYPHN010000181         8480 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000181 DAYPHN010000000
VERSION     DAYPHN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.181"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(143..219)
                     /locus_tag="ACG0BW_004151"
     tRNA            complement(143..219)
                     /locus_tag="ACG0BW_004151"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(183..185),aa:Asp,seq:gtc)
     gene            complement(352..1083)
                     /gene="dnaQ"
                     /locus_tag="ACG0BW_004152"
     CDS             complement(352..1083)
                     /gene="dnaQ"
                     /locus_tag="ACG0BW_004152"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFK7261050.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1148..1615
                     /gene="rnhA"
                     /locus_tag="ACG0BW_004153"
     CDS             1148..1615
                     /gene="rnhA"
                     /locus_tag="ACG0BW_004153"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFK7261051.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1612..2334)
                     /locus_tag="ACG0BW_004154"
     CDS             complement(1612..2334)
                     /locus_tag="ACG0BW_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFK7261052.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2368..3123
                     /gene="gloB"
                     /locus_tag="ACG0BW_004155"
     CDS             2368..3123
                     /gene="gloB"
                     /locus_tag="ACG0BW_004155"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFK7261053.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTRVVKDGEIAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3195..4553
                     /gene="mltD"
                     /locus_tag="ACG0BW_004156"
     CDS             3195..4553
                     /gene="mltD"
                     /locus_tag="ACG0BW_004156"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFK7261054.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4601..5371)
                     /locus_tag="ACG0BW_004157"
     CDS             complement(4601..5371)
                     /locus_tag="ACG0BW_004157"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFK7261055.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYTSGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5448..6248)
                     /locus_tag="ACG0BW_004158"
     CDS             complement(5448..6248)
                     /locus_tag="ACG0BW_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFK7261056.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6489..7403
                     /gene="yafC"
                     /locus_tag="ACG0BW_004159"
     CDS             6489..7403
                     /gene="yafC"
                     /locus_tag="ACG0BW_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFK7261057.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETLDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7400..8203)
                     /gene="dkgB"
                     /locus_tag="ACG0BW_004160"
     CDS             complement(7400..8203)
                     /gene="dkgB"
                     /locus_tag="ACG0BW_004160"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFK7261058.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHEFYITTKIWIENLSKDKLIPSLKESLQKLRIDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAKDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8366..8442)
                     /locus_tag="ACG0BW_004161"
     tRNA            complement(8366..8442)
                     /locus_tag="ACG0BW_004161"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8406..8408),aa:Asp,seq:gtc)
BASE COUNT         2262 a         2046 c         2061 g         2111 t
ORIGIN      
        1 ttaataaatg agacaacaag cgagaatcac gcacttacgc cagagtaaaa tcattgaaca
       61 gagaaatagc gggatttaaa tgcaaaaaaa ccgcaaaatt aaaaatctcg cggttctctg
      121 aactcatttt catgagtgaa tttggcggaa cggacgggac tcgaacccgc gaccccctgc
      181 gtgacaggca ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac
      241 gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg
      301 tttgctgcaa aaatcgccca agtcgctatt tttagcgcct tttacaggta tttatgcccg
      361 ccagaggcag cttccgccct ttttctgcac cagatcgaga cgggcttcat gtgccgcaag
      421 ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgcacaa tgcgctgaat
      481 tgttgtttca ccttgttgct gttgtgtctc tccttccatc gcaaaagcca tcgacgtttg
      541 accaccagtc atcgccagat aaacttccgc aaggatctgg gcatcgagta atgccccgtg
      601 caacgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg
      661 cttaccggga aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt
      721 ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa
      781 cgctgcgtta tggatcacca actccgcgcc gcgaatatag tccatgaact catcggctac
      841 ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc
      901 ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg
      961 acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcgcc
     1021 aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct
     1081 catagcggtc atttatgtca gacttatcgt tttacagttc gattcaatta caggaagtct
     1141 accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gtaatccagg
     1201 acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg
     1261 ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc
     1321 gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc gccagggtat
     1381 cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt
     1441 aaaaaatgtc gatctctggc aacgtcttga tgctgcgctg gggcagcatc aaatcaaatg
     1501 ggaatgggtt aaaggccatg ccggtcatcc agaaaacgaa cgttgcgatg aactggctcg
     1561 tgccgcagcg atgaatccca cactggaaga tacgggctat caagtcgaag tttaagcctg
     1621 tggtttacga cattgccggg tggctccaac cgcctggcga attcgtggct tgtttttact
     1681 ctgtttcatc ggatttagcg ttaaaggaat agtccgtttc cgggcgacaa taagttgtaa
     1741 gcaaccaagc gcaggaatat gcgcattcaa tagttttcca ccgtgtttgt tccacgggag
     1801 aacgtggaaa cggctggcgt gtagcacttc aaaattcaac aaagagagcc agtccagctg
     1861 ccgcatcaga gtaaacatcc ggctattata gggcgaggtt ttgcgcaata ccggcacaag
     1921 ttttcgtaat cccattaaac tgatgggatt gaagccacta atcaccagcc agccatcatc
     1981 aatcaatacc cgatcggctt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt
     2041 atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa gtgggtccgc
     2101 ctgcacctgg acgggcagtc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc
     2161 ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt
     2221 agtgaaccac gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa
     2281 atcgccccag caatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac
     2341 cttccgtttc acactgagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac
     2401 aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac
     2461 gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc catatttctc
     2521 acccaccatc accacgatca cgttggcggc gtaaaagaac tggtgaaaaa gtttccgcaa
     2581 attgtggtgt atggtccaca agagacacaa gataagggaa caacacgggt agtcaaagat
     2641 ggcgaaattg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc gggtcacact
     2701 ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct
     2761 ggtgggtgtg gtcggttgtt tgaagggaca ccatcacaaa tgtatcaatc acttaaaaaa
     2821 ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac cttatcaaat
     2881 atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa
     2941 gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa aaatgagcgg
     3001 caaattaatg tttttttaag aaccgaagat attgatttaa ttaatgtaat taatgaagaa
     3061 acattattgc aacaacctga agaacgtttt gcatggttaa ggtcaaagaa agataggttc
     3121 tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa
     3181 ctattgacac acacatgaag gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt
     3241 gccagagtac cggcaacgtt caacagcacg cacagagcct ttctgcagct ggtcaagggg
     3301 aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct atcgcgccag
     3361 atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg gaaaatgacc
     3421 ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac gatgtaactt
     3481 tacgggcaga gccgtatatg tactggatag ccgggcaagt taaaaaacgt aacatgccta
     3541 tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca acgtctggcg
     3601 ccaatgccgc aggcatctgg cagatcattc cgagcacggg gcgcaattat ggtttgaaac
     3661 agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaaca
     3721 tgatgcagcg tctgaacaag atgtttgacg gcgactggct tctgaccgta gcggcttata
     3781 acagcggcga aggtcgagtc atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca
     3841 cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg
     3901 cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca acgaccgatg
     3961 aaagccgtgc tctggcgcgt gtgcacctga gcagcccagt tgaaatggcg aaggttgcag
     4021 atatggcggg gatttccgtc agcaagctga agacattcaa cgctggcgtg aaaggctcca
     4081 cgctgggcgc aagtggtccg cagtacgtga tggtgccaaa gaagcatgca gatcaactgc
     4141 gtgaatctct ggcttcgggc gaaattgctg ctgtacagtc gacgctggtt gccgacaata
     4201 cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca agtatcgctt
     4261 cacgtctcgg cgttagcacc aaagatttgc agcagtggaa caaactgcgc ggatctaagc
     4321 tgaagccagg ccaaagcttg acgattggcg caggtagtag cgcacagcgg ttggcaaaca
     4381 acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc attgctaaac
     4441 gccacggcgt gaacatcaaa gatgtgatgc gctggaacag cgatactgcg aatctgcaac
     4501 caggcgataa gctgacgttg tttgtgaaaa acaacaacat gccagattcc tgacaaacca
     4561 gataataaaa aggcaccgat tcccccggtg ccttttttat ttatgctgct ttatgtgctt
     4621 ctaccatgat gatatcactg gtgaaagagc cgtcattctg caaggcaaaa tacgttctca
     4681 cctctgtcga tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac
     4741 gcattctcgc gacccatgaa gaaaattcca gaggtaactt atctgtaatt aaattatcaa
     4801 ctatcagatt ggcttcattg attaacgtca accactcacc gctggtgtag tttcgtacgt
     4861 gagaggtatc gcgtaatgct tctaccgtct gtaaccagat gtcgcgcact ggatgacccg
     4921 gagacattac gtccatcaca atcagcctac cgccgggttt caatacccga ttcacttcac
     4981 gcagtgctgc accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa
     5041 atgcgttatc ggcaaatggc agactttcgg cataaccctg gcgggtggtg atatttttca
     5101 gttgccgggc ttcggcagct tgtgccacga catccagcat ttgggcagat aagtcatacg
     5161 ccaccaccgc gctcacgttc tgcgcagcga caaagctggc atgccctgct ccgcagccca
     5221 tatcaagtac acttgcatca ggataatcag ccagacgcac cgccaggcgc tgtaaatctc
     5281 gcccggatgc atgtacggta ctggttaaat actcactggc ctgcgagcta aactgctttt
     5341 caacgtggtc atggtgggat tgtgttgtca ttgtgctgtc cttttattgt ttgattaaaa
     5401 agggcagcgc caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc
     5461 ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccagtaca
     5521 gaagcttcgc tgacgttcag accacggtag aaaacaaaat cgagcgggcg gccaaacgcc
     5581 cgacggcgct gatcatcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga
     5641 tataacgcgt tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg
     5701 ccgctgtggt gagctatctg atcgccaata ggaagtaact gcttactata gacatccacg
     5761 cccaaactga aattgacggc gtgtatatta accaccatca acaggcgggt gtcaggcaat
     5821 ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac
     5881 gggcagcaat acactggatg cgccgccgaa agggtcatta caccggaagg atgttgtggc
     5941 agcacgaaag cgggtacctg atcggcggca agatagttag cggtcgcaaa ctgcactaac
     6001 tctggcgttg tctgcgcttc ctgcaataac accagatgtg catctttgcc gtagttcttt
     6061 aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata
     6121 cgctcttcgg tacttaacgg ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac
     6181 cccggcggta agatcctttc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt
     6241 tttcgcactt ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc
     6301 tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc
     6361 aagtgcttac accggtcaga aatttgctca tgattgagaa cattaaatcg gatgacatat
     6421 cacgcatcct gatggtttaa tgacaaataa attcaaaata tctcatctat tttgcaacag
     6481 gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcggtc gtcgaaagcg
     6541 gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg
     6601 tgaaaaagct ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta
     6661 gcctgacgga agaaggcgag cgttattttc gtcgcgtaca gtcaattttg caggagatgg
     6721 cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg ttgcgcatcg
     6781 atgccgcaac tccagtggtg ctacactttc tgatgccgtt gattaagcct ttccgtgaac
     6841 gctatccgga agtcacttta tcactagtct cctccgaaac gattattaat ttgatcgaaa
     6901 gaaaagtgga tgtcgcgata cgcgccggta cgttaacgga ttccagctta cgtgccaggc
     6961 cgttatttaa cagttatcga aaaattatag cctcccccga ttatatttcc cgctatggga
     7021 agcctgaaac gctcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt
     7081 ccctcaatac ctggccgata gcctgtagcg atggacaatt acatgaggtg aagtacggtt
     7141 tgtcatccaa tagtggggaa acactgaaac agctttgcct aagtggcaac gggattgcgt
     7201 gtttgtccga ctacatgatc gacagagaaa tcgctcgcgg agaattggtg gagttaatgg
     7261 cagataaagt attgccagtg gaaatgccat tcagtgcagt ctattacagc gaccgtgcgg
     7321 taagtacgcg catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcctg
     7381 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
     7441 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttt ggcatcaagc tgtaaatttt
     7501 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
     7561 cttcccccat agcccatgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga
     7621 tacgagcaat aacctcatct ttcagggcct taccatacgc cagtgtcata taggaagtaa
     7681 tatggatgcc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
     7741 gttcaatctg gttagtagcg atgttttcag cgccaacagc agcaatcgcc ttttccatca
     7801 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
     7861 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
     7921 gcgtcagatc aacataatcg atacgcaatt tttgcaggct ctctttcaga ctcgggatca
     7981 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagaat tcatgacgtg
     8041 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
     8101 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
     8161 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatac ctcttttaat
     8221 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
     8281 acgcagagga tttttgcgat tctggcaata atagatatcc agacgaaaaa aaacctcgag
     8341 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     8401 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     8461 caccggtttt atgaccggct
//
LOCUS       DAYPHN010000182         8436 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000182 DAYPHN010000000
VERSION     DAYPHN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.182"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            101..1435
                     /gene="adeQ"
                     /locus_tag="ACG0BW_004162"
                     /gene_synonym="yicO"
     CDS             101..1435
                     /gene="adeQ"
                     /locus_tag="ACG0BW_004162"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFK7261059.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGIYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1488..1940
                     /gene="yicN"
                     /locus_tag="ACG0BW_004163"
     CDS             1488..1940
                     /gene="yicN"
                     /locus_tag="ACG0BW_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFK7261060.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2056..2379)
                     /locus_tag="ACG0BW_004164"
     CDS             complement(2056..2379)
                     /locus_tag="ACG0BW_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7261061.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2363..2716)
                     /locus_tag="ACG0BW_004165"
     CDS             complement(2363..2716)
                     /locus_tag="ACG0BW_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFK7261062.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2949..4133
                     /gene="nepI"
                     /locus_tag="ACG0BW_004166"
     CDS             2949..4133
                     /gene="nepI"
                     /locus_tag="ACG0BW_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFK7261063.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMLAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4335..>8436
                     /gene="air"
                     /locus_tag="ACG0BW_004167"
     CDS             4335..>8436
                     /gene="air"
                     /locus_tag="ACG0BW_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HFK7261064.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINPAHSDTAASLILPNVKTIPYTLGALESPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDNRTQTNHGIGWRYFTSSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKVVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLAGSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAETNGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSSVVEVSGTDKNETGNWSEESDGVYTSTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTAYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKINQSSDSKT
                     VNFIADVSTAQVAELVVTQDGSVADGSTANMLRVRVTDVFGNVLAGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGTSAVTASINNSSQSRNVTFIADVSTAQIADLVVTR
                     DNSVADGAMANTLRVRVTDAFGNALNGQTVSVLADNGATVTPTVTTEPDGTVEISITS
                     QTAGVSAVTATINSSTQSQNVTFIADVRTAKIADLVVIKDDSVADGA"
BASE COUNT         1970 a         1996 c         2299 g         2171 t
ORIGIN      
        1 tccattaatg tgcaattgaa atgtgataat tatcactgaa ccgttgttgt catttgtttt
       61 acaaaagcaa gggattaaat ctcctccaat ggacaaaaaa atgaataatg acaataccga
      121 ttacgtgagt aatgaatcag ggacgctttc gcgattattt aaactacctc agcatgggac
      181 caccgtccgc acagaattga ttgcggggat gaccactttt ttaaccatgg tgtacatcgt
      241 ttttgtgaac ccgcaaatcc tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac
      301 cacctgtttg attgccggta tcggcagtat tgcgatgggg atatttgcta acttacccgt
      361 ggcgctggct ccggcaatgg ggctgaacgc cttctttgcg ttcgtggtcg tgggggcgat
      421 gggtatctct tggcagaccg ggatgggcgc gatattctgg ggcgcaattg ggctattttt
      481 gctcacgctg tttcgtatcc gttactggat gatctccaac attccattaa gtttacgtat
      541 tggcatcacc agcggaatcg gattatttat tgccttaatg ggattaaaaa acactggcgt
      601 tattgtcgcc aataaagaca ccctggtgat gattggcgat ttaagttctc acggcgtgtt
      661 gttaggtatt ttagggtttt ttattataac cgtgttgtca tcacgtcatt ttcatgccgc
      721 ggtgctggtt tctattgtgg tgacgtcttg ctgtggatta tttttcggtg atgttcattt
      781 tagcggcatc tattccattc cgcctgatat tagcggcgtc attggtgaag tagatttgag
      841 cggcgcgtta acacttgaac tcgccggtat cattttctcc tttatgctga tcaacctatt
      901 tgattcatca ggaacattaa ttggtgtaac tgataaagct ggcttaatag atagtaacgg
      961 taaattcccc aatatgaata aggcgctata tgttgatagc gtcagttcgg tggcgggcgc
     1021 gtttatcggc acctcgtctg ttaccgccta tattgaaagt acttctggtg tggcagtcgg
     1081 tggtcgcacg gggctgactg cggttgtggt cggcgttatg ttcctgttgg ttatgttctt
     1141 ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc ggagcgttaa tctttgttgg
     1201 cgtgctgatg acttcgagcc tggcgcgcgt taactgggat gattttaccg aatcggtgcc
     1261 cgcatttatt accacggtga tgatgccctt tactttctcg attaccgaag ggattgcact
     1321 tggctttatg tcgtactgca tcatgaaagt gtgcaccggg cgctggcgcg atctgaacct
     1381 gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt attctggtgg attagtaact
     1441 ggcgagggtt tccagatatc atgagttctg attacgcagg agaactcatg atctggataa
     1501 tgctcgccac gctggcggta gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca
     1561 gaaaagcgat tcgccgtctc agcgatcggc tgaatatcga tgtcgtaccc gtggagtcga
     1621 tggtcgatca aatgggaaag tcagccggtg acgaattttt acgttatttg catcgtccgg
     1681 atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat ctggcaaatt gtcattgtcg
     1741 atggtagcga acagaatctg ctgcaatggc atcggatttt acaaaaagcc cgccttgccg
     1801 cgccgattac tgatgctcag gtcaggctgg cgctgggttt tctgcgcgaa accgaacctg
     1861 aaatgcagga tattaatgct tttcagatgc gctataacgc gttctttcag cctgccgagg
     1921 gcgttcactg gctgcattaa aaccgcttcg tcttatcagc ctacagaccg cgcctgacct
     1981 gtagggtgga taaggcgttc acgccgcatc cggtattgga actgacagta ggtgaatgca
     2041 ttatctacgt tctatttaca gcagcacctc cagcccgtgt ttttgcacaa tgctgagcag
     2101 ttttaatgaa atgccattgg ggcgtttagc gccactttcc catttttgta cagttgaaac
     2161 gctggtattg agatagtgag cgaagacggg ctgactcact tttaattttt cccgcagtgc
     2221 ttttatctca acaggttgta agtcttgcac gctggtcagg caggctttgt caaaagtacg
     2281 catcgtttcg gcagaaatag catcgacgct ggacaatcca gccgctgcgc tgtgaatagc
     2341 ctcaaatgca ggacttttaa atttagtttt tgcagtcata gcagatctct gtcagttctt
     2401 tagtgttaat cattgctgtt agttgggctt tcgtcaggaa ggcataatgc tttgcaattt
     2461 cacgaaaccc agccagttcc tgactattga tgttcgacat atcctgtttt gcatataaaa
     2521 acgtgtaaaa ccaattgctt cctcctttcg ccagaataat tgcacgctca cgattttggt
     2581 gaaggcgctt tttaaaaacg ccaccgccaa gtgcatcagc tttaccttgt tccgtttcta
     2641 aaatggcccg gcacaactct tcatctgtta ttgcatgaga cctggcttcc cttgcaaacc
     2701 atttggtttt aaacacgcgc atatcgttga ggtcctgtta ttaaagtata tcaccaagtg
     2761 caatacttta tcccttgttc ccaacacaat cgcgaaaata catatcacct tgccctatga
     2821 tttttatttc ttcgagcagc ctcgaacttt cccacaacac cgtaaaagtt gcaaaaacgt
     2881 taaatgcgtc acacatttca atgttaaagt ggtcggcttt tcccctgaaa catgccacgg
     2941 gtaacaccat gagtgaattt attgccgaaa accgcggcgc ggatgccatc acccgaccga
     3001 actggtcagc cgttttctcg gtggcgtttt gcgtcgcctg tctgattatc gttgagtttt
     3061 tgcctgtcag tttgttgacg ccaatggccc aggatttagg catttcggaa ggggttgccg
     3121 ggcaatcggt gaccgtgacc gcctttgtgg caatgtttgc cagtttgttt attacccaga
     3181 caattcaggc cactgaccgc cgctacgttg ttattttgtt tgccgttttg ctgacgctct
     3241 cctgcttgct ggtttccttt gctaactcat tcagtttgct tttaatcggt cgtgcctgtc
     3301 tggggctggc gctgggcggg ttctgggcga tgtcggcgtc gctgaccatg cgtctggtac
     3361 cgccgcgtac ggtgccgaag gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg
     3421 tgattgctgc gccgttgggc agttttttag gcgagcttat cggttggcgc aatgtcttta
     3481 atgcggcggc ggcgatgggc gtgttgtgta ttttctggat tatcaaatca ttgccttcgc
     3541 tgccaggcga accctcgcat cagaaacaaa atactttccg cttattacaa cgtccgggtg
     3601 tgatggcagg gatgctcgcc atcttcatgt ctttcgccgg gcagtttgct ttcttcacgt
     3661 atattcgccc ggtgtatatg aacctggcgg gattcggcgt ggatggctta acgctggtgc
     3721 tgttgagttt tggtatcgcc agctttattg gtacgtcgct ttcgtcgttc attcttaaac
     3781 gttcggtaaa actggcctta gcaggcgcgc cgttaatact ggctgtgagt gcgttggtgc
     3841 tgacgttgtg gggaagcgat aaaatcgttg ctaccggcgt ggcgattatc tgggggctaa
     3901 cttttgcatt ggttcctgtc ggctggtcaa cgtggatcac ccgctcactg gccgatcagg
     3961 cagaaaaagc cgggtctatt caggtggcgg ttattcagct tgctaatacc tgtggcgcgg
     4021 caatcggcgg ttatgcgctg gataatattg gtctgacttc gccgttgatg ttgtccggca
     4081 cattgatgtt gctgacggcg ttattggtca ccgcaaaggt gaaaatgaag taacgtattt
     4141 acaggagggg ttactgctct ggcgggaaag ctctcctgtt cttcccctga taaccattga
     4201 tcaggcattg ttcttatctt gttgttattt ccattattcc gcctgtattc atcatttgct
     4261 aatcatgaat catcattttc tctcatgtta aagaaaggtc atttaatctg aacgcatagc
     4321 gacagggatg acaaatggca gggaaagcgc atgggaatgg cgatcgtcgg ggcgataaca
     4381 ccatatgtgg attgggggat cgtttacgtc gtctgaccgc cggtatttgc ctgataacac
     4441 aaactatttt ccctgttatg gctgctgccc ctacgcatat taatcctgcg cactcggata
     4501 ctgccgcgtc actgatcctg ccgaacgtta aaactattcc atataccctg ggtgcgttgg
     4561 aatcgccacc aacggtcgcg gcacgctttg gtatcaccgt tgatgaatta cgtcgcctga
     4621 atcagttccg tacctttgcg cggggctttg acaatgtgcg tcagggcgat gaaatagatg
     4681 tgcccctcat taacagtaac agccctgaag cgcgcaacct gaaagcaatg cagatggagc
     4741 gcgacggcaa agatccgcaa atgcaggtcg cggaaatggc gcagcaaagc ggaacacttt
     4801 tagctcgcga tatggacagc gaacaggcgg cgagtatggc gcgtggctgg gtggcttctt
     4861 ctgcatctgc gcaggcaacg gactggctca gccgttgggg aactgcccgg gtatctcttg
     4921 gtgtggatga agatttcagc cttaaaagct cgtcatttga atttctccat ccctggtacg
     4981 aaacgccgga taacctggtc ttcagccagc acacacttca ccgtacggat aatcgcactc
     5041 aaaccaacca cggtatcggc tggcgttatt tcacatcgag ctggatgtct ggcgtcaata
     5101 tgtttatcga tcatgacctg acccgctatc acacccgaac cgggatgggc gttgaatact
     5161 ggcgcgatta cctgaaactg agcggcaacg gttatctgcg cctcagtaac tggcgtagtg
     5221 caccggaact cgacaatgac tatgaagcac gtccggcgaa cggttgggat cttcgcgccg
     5281 aaggctggtt accggcctgg ccgcagctgg gcggcaaggt ggtctatgaa caatattatg
     5341 gcgatgaagt ggcgctgttt ggtaaagacg agcgtcaaaa tgatcctcac gcgattaccg
     5401 caggtttgag ttatacccct gttcctctga tctcctttag tgccgagcaa cggcagggca
     5461 aacagggaga aaacgacacc cgaattggta tggagttgac gttgcaaccc ggccattcac
     5521 tgcaaaaaca gctcgatccg gctgaagttg cagcacgacg tagccttgtg ggcagtcgct
     5581 atgacctggt cgatcgtaat aacaatattg tcctcgaata tcgcaagaaa gagctggtgc
     5641 ggctgacgct gaccgatccg ttgaaaggca agccgggtga ggttaaatcg ctggtttctt
     5701 ccttacaaac caaatatgcg ctgaaaggtt atgacattga agccgcctcc ctgcaatcag
     5761 cgggcggaaa agtagctgtt tccggcaagg atattcaggt cactattcct ccctaccgat
     5821 tcacggcaat gcctgaaacc gacaacactt acccgatagc ggttacagcg gaagattcga
     5881 aaggaaattt ctcccggcgt gaagaaagta tggtggtcgt ggaaaaaccc acattaagcc
     5941 tcgccggttc aacgctttct gtcgatctgc aaatcctgct tgctgatgga aaatcaacct
     6001 caacgctgac ctataccgcg cgagattcca gcggcaagcc aatcccaggt atgacgttaa
     6061 aaacacaggt gaaaggtttg caggatttcg ccttaagcga atggaaagac aacggtaacg
     6121 gaacctatac ccaaatagtt accgccggga agacgtctgg cgcgttatcg ctgatgccgc
     6181 aatttaacgg cgatgatatc gctaagacgc cagcgctgat tgctatcgtg gcgaatacag
     6241 catcccgcgc tgactccacg attgagacag accaggataa ttacgtcgcg ggtaagccga
     6301 tagtcgttaa agtgaccctg agagatgata acggcaacgg ggttaccgga cgaaaagaac
     6361 ttctcaaaca aaccgttaaa gttgacaaca cgaaggctga tgccgttagt gcctggacgg
     6421 aagaaagtga gggaatttac aaagcaagct atacggccca tctcattggc gacaaattaa
     6481 cggcccagct gacgatgccc ggctggcaaa ccaaacattc ggatgcgttc agtattgcgg
     6541 gtgataaaga taccgccaaa atcgctgcga tgcagatcac cgcgaataac gcagttgcca
     6601 gacgcgatca caataccgtc gctgtgacgg tcagggatgt gcatcaaaat ttactgcagg
     6661 gacaaaatgt taccttcacg gtcgtgaatg gtgcggcggt ttttgccgat ccaaatggcg
     6721 gtattgtgac gacagataaa gacgggattg ccagcgttaa ccttgccagt gaccaggcgg
     6781 taaacagtct tatcaaggcc gagaccaatg gcagtagtca gtctgtggaa gtgagcttta
     6841 ttactggcga tatctcgcag ctgacctcca ccatcaagac agacgatgtg tcatacaccg
     6901 cgggtggcaa gataaaagtg tcagttaccc ttatggatga gcagaaaaac ctggtgaaag
     6961 ggatggcttc gctgctggcg ggtagcagtg tggtcgaggt gagcggtaca gataaaaatg
     7021 aaacgggtaa ctggagcgaa gagtctgatg gcgtttacac cagcacccga accgcgaaga
     7081 ttgctggcga tcgccattac gccacgctga aactatcgac atggagtagc gcgcagcaat
     7141 ctgatgcgta tgccatacgc gaaagcggag ctgtgttggc ttattcctcc atcgttaccg
     7201 acaaaacggc ctacaccgca ggcggtgcga taaaagttac ggtaacactc aaggatagct
     7261 atgaaaacct ggtcgggggg caacgagacg ctatcaacca ggctatccag ctgccaaata
     7321 caaaagcgga aagcatcgcc tggaatgaag atcaaaaagg aatttatacc gcaacgtata
     7381 ccgccttact ccctggcacc ggtttaaaag cacagctaca gatgtccggc tgggcgagcg
     7441 ctctgacttc aaatgattac agtattagtg gcgatgccgc cagcgcacaa attgtcgcga
     7501 tgcaagtgac aacgggtaac ccggatgtgc tggcgaatgg cagcgatcga catacggtga
     7561 atgtccgggt ggaagatcag tttggcaatg tgctgccgga gcagaccgtc acctttacgg
     7621 tgacgaaggg ggcggctgta tttgccaatg cagggcagag cgcagatatc aggacggatg
     7681 cgcatggcat ggcagaggtt gatctgagca gtaccgtagc ggatgccagc actgttgagg
     7741 ctaagatcaa tcaaagcagc gatagtaaaa cggtgaactt tattgctgat gttagcacgg
     7801 cgcaggtcgc tgagctggtg gtgacgcagg acggctcagt ggctgatggt tcgacggcga
     7861 acatgctgcg ggtgagggtg accgatgtgt tcgggaatgt acttgccggc cagacggtca
     7921 gcgtgttggc ggacaacggc gcaacggtcg ctccgacggt gattacagag ccggacggca
     7981 cggtggagat ctcagtcacc agccagaccg ccggaaccag tgcggtcacc gccagcatca
     8041 acaacagcag ccagagccgg aacgtgacgt ttattgcgga tgtcagcacg gcgcagatcg
     8101 cggatctggt ggtcacccgg gataactcgg tggctgacgg tgcaatggcg aacacgctgc
     8161 gggtgagggt gaccgatgcg ttcgggaatg cgctgaatgg gcagacggtc agcgtgttgg
     8221 cggataacgg tgcaacggtc actccgacgg tcactacaga gccggacggc acggtggaga
     8281 tcagtatcac cagccagacg gcgggagtca gtgcagtcac tgcaaccatc aacagcagta
     8341 ctcagagtca gaacgtgaca tttatcgccg atgtcaggac ggcgaagatc gcggatctgg
     8401 tggtcattaa ggacgactca gtggcagacg gtgcaa
//
LOCUS       DAYPHN010000183         8410 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000183 DAYPHN010000000
VERSION     DAYPHN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.183"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..271)
                     /gene="cas2e"
                     /locus_tag="ACG0BW_004168"
     CDS             complement(<1..271)
                     /gene="cas2e"
                     /locus_tag="ACG0BW_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFK7261065.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGVRLVS"
     gene            complement(268..1191)
                     /gene="cas1e"
                     /locus_tag="ACG0BW_004169"
                     /pseudo
     CDS             complement(268..1191)
                     /gene="cas1e"
                     /locus_tag="ACG0BW_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(1188..1838)
                     /gene="cas6e"
                     /locus_tag="ACG0BW_004170"
     CDS             complement(1188..1838)
                     /gene="cas6e"
                     /locus_tag="ACG0BW_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFK7261066.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LWRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(1820..2566)
                     /gene="cas5e"
                     /locus_tag="ACG0BW_004171"
     CDS             complement(1820..2566)
                     /gene="cas5e"
                     /locus_tag="ACG0BW_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFK7261067.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNVFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYHQYQEHFHDLKIALPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(2577..3632)
                     /gene="cas7e"
                     /locus_tag="ACG0BW_004172"
     CDS             complement(2577..3632)
                     /gene="cas7e"
                     /locus_tag="ACG0BW_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFK7261068.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3647..4183)
                     /gene="casB"
                     /locus_tag="ACG0BW_004173"
                     /gene_synonym="cse2"
     CDS             complement(3647..4183)
                     /gene="casB"
                     /locus_tag="ACG0BW_004173"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFK7261069.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(4180..5742)
                     /gene="casA"
                     /locus_tag="ACG0BW_004174"
                     /gene_synonym="cse1"
     CDS             complement(4180..5742)
                     /gene="casA"
                     /locus_tag="ACG0BW_004174"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFK7261070.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLESEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRHIE
                     EGQDADELLSKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(5840..>8410)
                     /gene="cas3"
                     /locus_tag="ACG0BW_004175"
     CDS             complement(5840..>8410)
                     /gene="cas3"
                     /locus_tag="ACG0BW_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFK7261071.1"
                     /translation="DVAAMGYLMVKSNCFGLADYFRQLEISDKEQAAQFFAWLLCWHD
                     TGKFARSFQQLYLHPKLRVPEGARKNYEKISHSTLGYWLWHHYLSECQELLPSSSLSP
                     RKLTRVIAMWMPITTGHHGRPPDRIDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAF
                     WDDDEGIELIKHLSWYISATVVLADWTGSSARFFPRVAQAMDIKDCWQKALVQAQNAL
                     TVFPPKAQTAPFTGINTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEA
                     ALILAHRLMAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSART
                     LMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVM
                     PFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQ
                     CDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVHSHRVATRKDVERSVSVGWLH
                     SEQECIARIESAVSQGKCIAWIRNSVDDAIKVYRQLLARGVIPASSLSLFHSRFAFSD
                     RQRIETETLARFGKEDSSQRAGKVLICTQVLESSVDCDMDEMISDLAPVDLLIQRAGR
                     LQRHIRDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRI
                     WLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKF
                     VLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIASVVKPYANGAHAWEMSVVRV
                     RRSWWKKHRDEFSLLEGEAFRQWCIEQRKDPEMANVILVTDDESCGYSAMEGLTGKVG
                     "
BASE COUNT         1996 a         2341 c         2028 g         2045 t
ORIGIN      
        1 aagaaaccaa acgcacccca tccaaatcca ccggaatacg tctattttca ccccaggtct
       61 gaaattcaaa acccgactcg gtattggtcg cccaggccat taccacattt ccgcaacctg
      121 ccagttgaga gatttgctgc cagatcatct cccgaatacg tttggacgta tctccgacat
      181 aaacaccggc acgcacttcc aggagccaga tcgcgagccg tccacgtaag cgcggaggga
      241 cattttctgt aacaacgacg accatactca tccgccgcgt ccccggtgac cgctatcgcc
      301 cagcgtttca ggttcaggga tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc
      361 aatttcacct gcggcaagca cttcctcgat taacggaatt aatttgcccg ttaacttact
      421 gctacggaaa atatcacgac aggccaacct gacttcttta tctggttctg cgggttgcct
      481 cgctgctatt tcaaatgcct ttggcacaac cgaatcaaat ttaatgatat cggctatgtc
      541 ataaacaaat gaaagcggtt tgccactatg gataaatcca atagcgggcg cttatcccgc
      601 ggctaatact gccgcttcag aaataccgta cagacatgat gtggcagcac tgatgcagcg
      661 attcacaaca tcgccttttt cccagtcttt aggatcgtat ttgcgaccat tccatttcac
      721 accatattgt ttcgccagta atgcatacgt ctggcgaacg cgggatccct caattccacg
      781 tagctgctcc actgaacggc gagctggcgg tggctcacga aaacgtaatt catacatttt
      841 gcgcaccacc ttcagacgta gatcttccgt taaagccagc tttgcctggt agagtaactt
      901 atcagcacgc gccccaccgg gttgtccgga agagtagacg cgaacacccg cttcaccgac
      961 ccagaccagt agcgtcccca ccgtggcggc cagatgcacc gccgcgtggg aaactctcgt
     1021 tcccggttcg agcataatgc aggcgaccga tcccaccgga atgtgcgtgc ggatcccggt
     1081 tttgtcgatc agcacgaaag cgccgtccag tacgtcgatt tgaccgtact ggaggaagat
     1141 catagaggtg cgatctttta acgggatcgg actcagtggt acaaacgtca cgcctctgct
     1201 ccgggtttga tcagcatcag accacagccg aacgcgcggc ttttgccata cccctggctc
     1261 agacgttgca gaaataaccc gggatcggtg accgtaagca tccccgtata atccacgcta
     1321 ctgaactgga tcagttgccg ggagttttcc cgccacagtt gctgttgtcg gtaggcatca
     1381 accgaggtat ccagcagtgt aaaaccgctt ctctccccct gcgctgccag ccagtccagc
     1441 gccgcctgtt gttgatgcaa ccagacatca cttccttccg cctgccccct cacctgccgt
     1501 ttcgcctcca tcagcagatc gtggcgcttg cccgctttac agattgttgg attcgcccgc
     1561 aggttgaaac aaagttgttg tccggtacgc agctcgggca caaatgaccg gcattcgatg
     1621 gtgaacgttt cactttctgc cgggcgttcc tgcgacagta caaaaaagcg aaacgcgccc
     1681 tggagttctt cccgacgata aagaaattgc ctttctttgc cgccagggaa gagatcccac
     1741 agccactgat gcatcacata ttccccgcga tccaccagat gcagcaactg cgcaggcgaa
     1801 agctggccgg tatgtaaggt tattcttgag aggtacatgg ttcctccttg ctgagccacg
     1861 gcccctgatt gacggtgcgt tccccaaaca accattgctg acgatttaaa ggaacatccc
     1921 ggcggcgcaa tattttgctc gcgaccaggc cgtcgtgctc cccttcccac cagcattcat
     1981 cctgaagttt cgggagtgcg attttcaggt cgtggaaatg ctcctgatac tggtgatatg
     2041 cgttacgtaa aacatcagac gcgttgcctt caagcagtaa cggcgcaagt ggtaatgcca
     2101 gcggatgact ttttcgcccc agataaagcg gaaaaactgg atggcgtaaa ccgccctgca
     2161 actgttcgag gctgtaaggc gcatcggctg ttgttgccac cgccaccatc caccaggcgt
     2221 cggtgtagta gtcgcgccgg gagataatcg cgctcagaag atcagggtcg ctcaactctt
     2281 cgcgacggct gaaataacgc gctttacgca cctcttttgg catctggatt gtgtgataat
     2341 cccgtgccca gcgcgggtta cggctggcgc aaaccaccag tgaatagtgg cggttaaaca
     2401 cgtttaatcg ttcggtatca tcacgccgaa tccctacccc ggcagccagc agccccagca
     2461 atgctgagcg cgaaggcagt tcatgggtat gacgcacttc gccgggggca tcgacgcccc
     2521 aggatgccat tggcccatga agctgaaaaa tcaaatattg gctcattagc cgccccttac
     2581 gcgcagataa agtccagcac gtccttcatg cttccctgtt tgttcatcac gtcaaagctt
     2641 gcgcattcgg tcttctgttc atagaccgta ttcatatttt cgcgaagcgt tgtaatacgc
     2701 tgcaccgcca catctaactg ccgggtgcca ttaatgggtt catagaaagc cgccgccaga
     2761 gagcgtggtt gttcagtgcc tttttctgcc agcgcccagg aggcgtaggc acggctggca
     2821 aagctgttct gtttgccggt tggggagact ttaagtgcgg cttccgtaaa ggcgcgcaag
     2881 gtctgattag ctaacgcttc gtcaccgccg aggttttcga ccagcagatc tttatcgatg
     2941 cagatatagg tgtagaacag cgcagaaccg aatccggttt caccaagatg cccggcaccg
     3001 gcatcttcag aagcctggcg caaatcatca acggcggtga aaaaatcatc ttcgacaatc
     3061 gtttcactga caccaaatgc atgcgcgacc tggcaggcgg cttcaacatt aaattcgggt
     3121 ttattcgcca gcatacgacc aaacatagcg atatctaccg ccatgcgatc tttacgtaac
     3181 aaagcgagat cttcctcttt tggcgcgcgc ttttcttcgg ccagttgatg ggccagcgct
     3241 tttacggcgt caaattctgc cgggctgata tggactagct gttcagtttc ggcgttagtg
     3301 agcggatctt ttggtttttt gtcgttttta gctttcccaa gataatccgc aatttttgcc
     3361 gcccattcga tggctttttt ctcttcgatg cctttctcaa tcaggatagt tgccgcctca
     3421 cgcgcaatac gcccactgcg aataccaata tggcccgcca gtgcctgttc aaaaagtgca
     3481 gaagtgcgcc acgcacgttt cagactttgt gaggaaacgc gcagacgcgt tgctccaccc
     3541 agtaccacag tttttggcgc accggtgtca tcacggttaa ggttggcagc agggtaagcg
     3601 gttaacaaat gaagctgaat aaatgtcgtc atgagatagt cctttattaa ttttgttcgt
     3661 tatcggcgtc gccagcctgg taatattcca gcgcccagcg gatacggata aattctgttg
     3721 gccgctgctg gcggcggtga tgattgagca ggtcgtcgct ctcctggcac cagcggaaaa
     3781 tatcttctgc caaagagata agattgacgg aaccatttaa taatttgacc gcgcgacgta
     3841 actgacgcag taaatcatca ggcgttttta ctgccgacag gcgagtaaaa cgcccctccg
     3901 acattaccgc cgccagctgc gcggcaaaag gctgccgttc atcaatagct ttaacattgg
     3961 cgctaactgc ggcgaccagc gccagtgcgg taaaacgcca ctcttgtgct tcaattttcc
     4021 acaaagtgtg ggtctgcatc agcagagaac gaaacccttc tgacagacaa acatcattaa
     4081 ccgttgtgct acgccgcaga ctggcacgat tgccgcgttt ttcctgcaac tcttcgtgcc
     4141 atttacgcag cgtggctttg tgctcctctt ttacaatact cattcagcag cctcctgctt
     4201 tttctccctg gcggctttag cattttgctt ctccgccgat gttgtaaaat atttcttgcg
     4261 cgcggtcatg acgcgtttca aatcaacggg ctcatatgga ttggtgaaga cacgctcgtc
     4321 aaaatcctga cgcgcgaata accaaatttc cttttgccat ttgctgagta attcatccgc
     4381 atcctgacct tcttcaatgt ggcgcactaa cctcaggaag cgatgctgag ttttgttcca
     4441 gaagtcgata tccacaaaac tgaaatcacc ccgtgcacct tttggatcgg agaaccacgc
     4501 ttctttcaat gcactccgta acagactcag aatccgtgaa gccgtttgcg cagccagccg
     4561 cagcttcggt atctggcctt cttttttatc gagcagcagc ggaaaatggt gttcgtacca
     4621 acagcgcgct ttcatgttgt caaaatcata accaaatccc cacaggccca cttttgcctg
     4681 cttgagactg ctggcattaa agagtttcac caccagcgcg ggaagttccg tattgttttc
     4741 tgatttaccc gtttcgataa ggcctaacca gtcgcgccag attaaaccgc ctggttgtgg
     4801 tttgaccgag taaaactcgc cgccctcttt aaggggtaca cggtaaggcg ttaagggatg
     4861 ctgccacatg gcataattcg cgccgtaatt tttggtcgtc atcaggctca ggagcgcgtc
     4921 gttctgctcg ccgcaaatat cgcagttgcc gactgtcgtg gtattaaaat caatacgaat
     4981 acgccgcggc attccccagt acgcctggag tttattaacc tgatcatcgg ttaccaccgc
     5041 accggccagt tcgctggtac gcgtcgggcc aagccagggg aaaaccagat cgtcaaattt
     5101 tttgggtagc ggtaagtcgg cttcgtcctg cggcatcaca ttgagccaca gtttgcgcca
     5161 caagggcgtt tgttgattgc cctgatattc ctgtaattca atcagagtgg tcatcggccc
     5221 accgccgcgt aaaccggtgc ggtagccttt accacctgac ggcgcattta actgtaggga
     5281 gaacagagct aacgcagaac aatgtgggca caggtattcc gttacaccac gcttgataaa
     5341 gtggtcttta ttaaacttcg ttgtttgagc gccgggaatc tcaggcagta gcgaagcgac
     5401 ctgaacttta tctcctgtga gcgcctcgaa atcctgcata aatgaagggg aatctggccc
     5461 aaactggaaa gcgtgttcta aggacagcaa tgcttcccgt agcttttcag attccagtcc
     5521 gtcttcccag atatcatccc aacgacgatg atcttttggc gcaaagctgg tttgtagtag
     5581 ccccagcaaa aactgccatg ccgctccctg gagatctgcc cgtggcgcag caatatcgac
     5641 aacattttca tccgccagat cgacaggcgc cagtttgcct gtcgttccgt ctttataacg
     5701 aacgggcaac cacggggttg tcagaagtga aaacgagttc atacgcatcc tctcgtaaaa
     5761 aagaccatcc taggtcggtt gtccatcatt ccatcctttt cggtgtatca actattcaga
     5821 gagccctgtc gcaggcatct caaccaacct tgccagtcaa tccttccata gccgaatacc
     5881 cgcaactttc gtcatcggta actaaaatca cgtttgccat ctccggatct ttccgctgtt
     5941 caatgcacca ctgcctgaac gcttcccctt ccagtaaaga aaactcatcc cgatgttttt
     6001 tccaccagct tcgacgcact ctgacaacgc tcatttccca tgcgtgagca ccgttggcat
     6061 aaggcttcac cacactggca atacaggtag ccagccacag ggaaacagac tcctccgcca
     6121 gacgtgtcga cagcttttcc ggaaggtaat cgttgatatt ggcggcatag ccaggcctga
     6181 agttcagcac aaacttttta gccattgcgc gatcgcagta atatttgccc acttgctcct
     6241 gctcgctgcg ggcaaatcct tccggcatta ccacgtcctc accgtagact gattcaataa
     6301 gaaggcgggc tgcgtgtggc atttgaatag cgccttgctc acgcagtaca cgctgcgtca
     6361 gccagattcg tccatgatcg ggatagacat atgcactgtt acgcatggca ctgccgaacc
     6421 attcgtcacc tggagcgtcg tcccagacgg gggccagaat cagcaattca ggaggggaac
     6481 gctcgtcttt tccgtcacgc tttaactgac cattaatatc gcggatatgc cgctgtaatc
     6541 gccccgctcg ctggatcagc aaatcaacag gggccaggtc ggaaatcatt tcgtccatat
     6601 cgcaatcaac gctcgactct aagacctgag tacaaatgag gacttttccg gcacgctgtg
     6661 agctatcttc tttaccgaag cgtgccagcg tttccgtttc aattcgctgg cgatcgctaa
     6721 aagcaaagcg gctatgaaac agagaaaggc tggaagcagg aatgacgccg cgggcaagca
     6781 actgacgata aaccttaata gcgtcatcga cagaattccg gatccaggcg atgcatttcc
     6841 cctgacttac tgccgattcg atacgcgcaa tacattcttg ttcgctatga agccaaccca
     6901 cgctcacgct acgctcaacg tctttgcgcg tcgctacccg gtgtgaatgc acatcggatt
     6961 tcgtaacgtg cgtcagccag gggtaatcat ccttttccag gaacggagct tcttgctggc
     7021 cctctgtacc acgcgcaaag gcagcaacga gtttgtcgca ctgctgttga gataacgtgg
     7081 cagaaagcaa aatgacgctg tttccgccac gcgcctgccg ctcgatcagc ccttcaagaa
     7141 tgcatgacat gtaagcgtca caggcatgga tctcatcagc cagcaggatt ttgttactca
     7201 gtcccagaag ccgcagatta ttatgtttga acggcatcac agccatcatc gcctgatcca
     7261 gcgtgccgac gccaatttca gccagtagcg ccttcttgtt actgtcggca aaccaggccg
     7321 cacatccctg actgaacgtt tgttcatccg gttcttctga cccgactaaa tcaccggacc
     7381 agagtgattc attaaagcgg tccattaatg ttcgggcact gtgtgccagc accaggctgg
     7441 ggcgggactc tggcgaatag aaagcaagcc aggttttgac cagtcgatcg tacatggcat
     7501 tggccgttgc cattgttggc aggccaaaaa acaaaccctg cgctttaccc gcagccatca
     7561 acctgtgcgc caggataagc gccgcttccg ttttacctgc gccagtcaca tcttccagaa
     7621 taaataactg tggccctggc tggctgatat ccagatccag caccttttgc tgtaacggtg
     7681 tcggattctc aataaaagga aacagcgtat taattccggt gaaaggcgcg gtttgtgctt
     7741 ttggaggaaa gacggttaag gcgttttgag cctgaactaa agccttctgc cagcaatctt
     7801 taatatccat tgcctgtgca acgcgtggaa aaaaccgcgc tgacgatccc gtccagtctg
     7861 cgagtacgac ggttgcagag atataccagg aaaggtgttt tataagttca atgccctcat
     7921 catcatccca gaatgcgggg atctctatga gcggaaacag tggtttgatt tcaagaagaa
     7981 aatctcgcgc ggcagctttg tcttcaggca gaaaattatc cagttcatca atacggtcag
     8041 gcgggcgacc atgatgcccg gtagttatgg gcatccacat cgctattaca cgtgtaagtt
     8101 tgcggggaga gagtgaagag gaaggaagta actcctgaca ttcacttaaa taatgatgcc
     8161 acaaccagta gcccagcgtt gaatgagaga ttttttcgta attttttcta gcaccttccg
     8221 gaaccctgag tttcgggtgc aggtaaagtt gctgaaatga gcgggcaaat ttaccagtat
     8281 cgtgccagca caacaaccag gcgaaaaatt gagccgcctg ttccttgtca gaaatctcta
     8341 attgacgaaa atagtcagcc agtccgaagc aattgctttt aaccattaaa tagcccatcg
     8401 cggctacatc
//
LOCUS       DAYPHN010000184         8273 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000184 DAYPHN010000000
VERSION     DAYPHN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.184"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..609
                     /locus_tag="ACG0BW_004176"
     CDS             <1..609
                     /locus_tag="ACG0BW_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFK7261072.1"
                     /translation="SKYQHLLFKKKLEHKITRNWSNPKYMSFLYAQFIRKDLSSAPAV
                     IVKKPQKRNHPEVNFEEITDNRDLIGKKSEEYALNWEKNRLIGLGYSKLAEEIDDRRN
                     RPTYGYDFLSFNAPGDERYIEVKSIGRDGKEGAFRFFLSGNELTVSNLSNHRKNYYFY
                     LVQYGKDGEPCNLYVKHAQDLYTNSEMTPCAYVVRFDLEEPA"
     gene            complement(681..1316)
                     /gene="hxlA"
                     /locus_tag="ACG0BW_004177"
     CDS             complement(681..1316)
                     /gene="hxlA"
                     /locus_tag="ACG0BW_004177"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HFK7261073.1"
                     /translation="MKLQLALDELTLPEALVFIDKVVDDVDIIEVGTPFLIREGVNAI
                     KVIKEKYPHKEVLVDAKIMDGGHFESQLLFDAGADYVTVLGVTDLLTIQSCICAAKEA
                     GKQVVVDMICVDDLPARVRLLEEAGADMLAVHTGTDQQAAGRKPIDDLITMLKARRKA
                     RIAVAGGISSQTVKDYALLGPDVVIVGSAITHAADPADEARKISQVLLQYH"
     gene            complement(1340..1903)
                     /gene="hxlB"
                     /locus_tag="ACG0BW_004178"
     CDS             complement(1340..1903)
                     /gene="hxlB"
                     /locus_tag="ACG0BW_004178"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFK7261074.1"
                     /translation="METQSVAGVACTDLHQAMSRIDGAALARLEQAIAEANTVFVFGA
                     GRSLLMLKAFAMRLMHIGLKVHVVGDVVTPALKNGDLLLLASASGETASLVNVATKAK
                     QLGGTVALLTIFPESTLGNLAEVVVRIPAYTDKLPDGLDNVKGILPGGSLFEEAVMVL
                     GDAMIVNLAQSTGYRLTKGFALHANLE"
     gene            complement(1903..2745)
                     /locus_tag="ACG0BW_004179"
     CDS             complement(1903..2745)
                     /locus_tag="ACG0BW_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HFK7261075.1"
                     /translation="MKQLHHSGLPLYLDDDGVMVLKPPLNYLGFGRKSAGQMAVVLPE
                     FTEAQRDEPAYDVYRGLSFAEDQERLAADQYQYDITIIMSGTIGRERKKTSGHYHGYN
                     DTRRNTHPELYEVIKGTAAYILQKSPDFAVAPKDLLVDDLIVAVVKEGESIIVPPNYG
                     HCSINIGDGPLVFSNLAYKPCAVHYDTVQFYHGMACYIVEENGQLCVRKNHYYPHIPR
                     IKFATVKGNPHLGITFDTPLYQRYRAAPERFHFLGHVDNYVREIMGMLQYEDDLFPLC
                     QEDA"
     gene            complement(2742..4268)
                     /gene="ptsG"
                     /locus_tag="ACG0BW_004180"
     CDS             complement(2742..4268)
                     /gene="ptsG"
                     /locus_tag="ACG0BW_004180"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFK7261076.1"
                     /translation="MKRFNLLQMLQSIGRSLMIPIAMLPAAGILLAFGVSFQDPNIVA
                     SLPFLGADWLVHVLKLMAEAGSAIFANLPLLFAVGVAVGLSDDQGIAGLSAIAGFLIM
                     NVTIGQFLGITPESVAQVRDYTMVLGIPSLQTGVFGGIIIGIIAAWLYKRYYRIQLPS
                     WLEFFSGKRFVPIVTSFAALFVGLVMTVVWPPVQHLINGLSNTMTVQGPGVSAFLFGF
                     VERLLIPFGLNHVWWPTFWLQFGEYVNKAGQVVHGDQLIFFAQLKDQVPITAGTFMAG
                     LTPIKMFCIPAIAVAIYRCASPENKARVKGIMLSGAITSIVCGITEPIEFSFLFVAPV
                     LYGIHAVLAGLVFLLMEWFSVHIGLSFSGGLIDYLFFGVLPRAPHWYMVFPVGLVMGA
                     VYYALFTFAIRRWNLLTPGCEVEESTVAQENEQNDLVSGIILAYGGLGNMTRIEACMS
                     RLRIDVTDKSLVDKALLKQLGAAGVVEVGNNIQSVFGMKSDRLKEAIRAANAYTQEAK
                     "
     gene            4491..6170
                     /locus_tag="ACG0BW_004181"
     CDS             4491..6170
                     /locus_tag="ACG0BW_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFK7261077.1"
                     /translation="MSIFEAHFRRLHARYGAGQTHELQMQEIAAIFGCSVRNCRIALK
                     KMHQEKWLDWQPQRGRGKRSRLHLLTSPEKLFSQNVNKLLEKQDYGNVLRFIGNDKYL
                     LDRLSLWRFGVQDKSSETRVRIPYYRNLDPLNPLVPLRRTERHLLRQCLSGLTRYDAV
                     QGRIIPDIAHYWTHNDDFTRWEFWLKSTARFADGSELNASAVQRCLLAASQSPQFAPL
                     FSPIKTITADAPWHLVIETYHPVRRLDCLLATQPTMLFDYQHGHIRCTGAFHLQEHSD
                     NFMVLRRNQHWHQARPGLDEITIFTWAPEHISMSFIPLLRGEEAQDDRPLNERSLEQG
                     CCFVLLDGEGAFADEAGRRFINYLLQPVELLSQTQLPDEYARILSVAQGMLPQWNHRP
                     VDFGGITAPFNLRQPVIISTFQQPELVELAGAIRILLERWHIRAEIRIDAFDSFNSQP
                     RPPADIWLSNFMLDTLSVPAFLEWLASTTLFTRLPETQRQNLNALLPTILNSDNEQAF
                     ATIAAFFHEMTHQRYVIPLLHHWMEFATEKSFTWRDLNTLGWPDFSQLWLE"
     gene            complement(6323..6436)
                     /locus_tag="ACG0BW_004182"
                     /pseudo
     CDS             complement(6323..6436)
                     /locus_tag="ACG0BW_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6522..7058)
                     /locus_tag="ACG0BW_004183"
     CDS             complement(6522..7058)
                     /locus_tag="ACG0BW_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFK7261078.1"
                     /translation="MVTTHVFIAVSLDGYIARQDGDIDWLLKRDDPTEDHGYAAFIAD
                     KDWIIMGRGTYEKVLTFDEWPYDRPVLVLSRQLTDTPVPKALKGKVQFSCRTPREALA
                     DLMEKNVHRVYIDGGQLIQSFLREGLVADIVITTVPVLLGSGKPLFGVLPHDIDLTLL
                     SSRSFPSGLVQSHYRLTA"
     gene            7283..>8273
                     /locus_tag="ACG0BW_004184"
     CDS             7283..>8273
                     /locus_tag="ACG0BW_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosaminoglycan attachment site"
                     /protein_id="HFK7261079.1"
                     /translation="MSSLNLFDDVVPLEKQHPIYIMMKKERYEPEREVINQWAKGFLD
                     RDNKFTKEFQTSFEPCLWELYLFAYLKELGLRNDFSYDAPDFIVNEPGFCIEATIALP
                     AQGAPGAHGFSTEDMPRDFNKFNSEASIRLSNSFISKVKKLRSRYSQLPQCKEKPFVI
                     AIASFDRPFAHFAAARPILATLYGLYHDEEATIESGSKSIISYNVEAAVKKENVNIAM
                     GLFCTPDYSDVSAVIYSSLATWGKVRALADNPSALTIYTTFTPRENSLYPKVHQAKKN
                     DYIEHLADGLYILHNPFAKYPLPKETLSHPRVAQGYVESDGYVNFVAPEDFLLL"
BASE COUNT         2122 a         2256 c         2009 g         1886 t
ORIGIN      
        1 agtaaatatc aacatttgtt attcaagaaa aaactagagc ataaaatcac ccgaaactgg
       61 agcaatccaa aatatatgtc cttcctttat gcccagttca tacgtaagga tcttagcagc
      121 gctcctgctg tgatagttaa aaaaccacaa aaaagaaacc atcccgaagt caatttcgaa
      181 gaaataacgg acaatcgtga tttaataggc aaaaaaagcg aagaatatgc attaaactgg
      241 gaaaaaaacc gcctaatcgg tctcggctat tcaaaactag ctgaggaaat agatgatcgc
      301 cgtaatcgtc caacttatgg ttacgacttt ctttctttta atgccccagg tgatgagcga
      361 tacatcgaag taaaatcaat tggccgagat ggaaaagagg gagcattccg ttttttcctc
      421 tcaggaaatg aactcaccgt ttctaattta agtaaccacc gtaaaaacta ttatttttat
      481 cttgtacagt atgggaaaga tggagagcca tgcaatctat atgtaaaaca tgctcaagat
      541 ctttacacta atagtgaaat gaccccttgt gcttacgttg ttaggttcga tctggaagaa
      601 cctgcttaat agtttcgaca acctccgact cataacttta aaagtgtgaa atcaggcgca
      661 gagtcacctg agccttcatc tcagtgatac tgcaacaaca cctgcgaaat cttcctcgct
      721 tcatcagcag gatccgctgc atgcgtgatc gctgatccca caatcaccac atccgggcct
      781 aacagcgcat agtctttcac cgtctggcta ctgatgccgc ctgctacggc aatccgtgct
      841 ttccggcgcg ccttcagcat tgttatcaaa tcatcaatcg gcttgcgccc tgccgcctgc
      901 tggtcggtgc ctgtgtgtac cgccagcata tccgcgcccg cctcttccag gaggcggacc
      961 cgcgcaggga gatcgtcgac gcagatcata tccaccacca cctgctttcc agcctccttc
     1021 gccgcacaga tgcatgactg gatcgtcagc aggtcggtca cacctaacac cgtgacatag
     1081 tctgctccgg cgtcgaaaag cagctgcgat tcaaaatggc cgccatccat aattttcgca
     1141 tccaccagta cttccttatg cgggtatttc tctttaatga ctttaatcgc gttcacgcct
     1201 tcccgaataa gaaaaggtgt acccacttca ataatatcaa cgtcgtcaac caccttatcg
     1261 ataaatacca gcgcttcagg cagggttaac tcgtccaggg caagctgtaa tttcatgttg
     1321 gtttccttgt gtcgaatgac tattcgaggt tggcgtgtag cgcaaaacct ttggttaagc
     1381 gatatcccgt cgactgcgcc agattcacaa tcatggcatc gcccagcacc atgaccgcct
     1441 cttcaaacag gctgccgccg ggcagaatgc ctttcacgtt atcgagtcca tccggcagtt
     1501 tgtcggtata ggccgggatc ctgaccacta cctccgccag attccccagc gtggactcgg
     1561 ggaaaatggt cagcaaggcc acagtgccac ccagttgctt cgctttcgtt gccacattca
     1621 ccagcgaagc cgtttcaccc gatgcgctgg ccagtaacag caggtcgcca tttttcagcg
     1681 caggcgtgac cacatcgccg accacatgca ccttcaggcc gatatgcatc aggcgcatcg
     1741 caaaagcttt cagcattaat agcgaacgcc ctgcgccaaa aacaaacacc gtgttcgctt
     1801 cagcgatggc ctgctccaga cgtgccagcg cagcgccatc aatccgcgac atcgcctgat
     1861 gcaggtcagt acaggcgaca cctgcaacag attgcgtttc catcatgcgt cctcctgaca
     1921 gagcgggaat aaatcatcct catactggag catccccatg atttcccgga catagttatc
     1981 gacatgcccc agaaagtgaa aacgttccgg tgcggcgcga tagcgttgat aaagcggcgt
     2041 atcgaaggtg atgccaagat gcgggttccc tttgacggtg gcgaatttga tgcgcggaat
     2101 atgcgggtag taatgattct tgcgcacgca gagctgcccg ttctcttcca cgatgtagca
     2161 ggccataccg tgataaaact gcaccgtgtc gtagtgaacc gcgcagggtt tataggccag
     2221 attgctgaac accagcggcc cgtcgccaat attgatggag cagtggccgt aattcggcgg
     2281 cacgataatg ctttccccct ccttcaccac cgccacgata agatcgtcca ccagcagatc
     2341 ttttggcgcg actgcaaaat ccggcgactt ttgcaggata tatgccgcag tccccttaat
     2401 cacttcatat aattccggat gggtgttgcg ccgcgtgtcg ttatagccgt gataatgacc
     2461 gctggttttc ttgcgctccc taccaatcgt tcccgacatg atgatggtga tgtcgtactg
     2521 atactggtcg gcagcaagcc gttcctgatc ctccgcaaaa ctgagcccgc gataaacatc
     2581 gtaagccggt tcatcacgct gcgcttcggt gaactccggt aagaccaccg ccatctgccc
     2641 ggcgcttttg cggccaaagc cgagatagtt cagcgggggt ttcagcacca tcacgccgtc
     2701 gtcatcgaga tacagcggta agccgctgtg gtgtagctgt ttcattttgc ctcctgtgta
     2761 tacgcgttag ctgcgcggat cgcctctttc agccgatcgg atttcatgcc aaagacgctt
     2821 tgaatgttgt tcccgacctc gacgacaccc gccgcaccaa gctgtttcag cagcgctttg
     2881 tccaccagcg acttatccgt cacgtcaatg cgcagacgcg acatgcaggc ttcgatgcgg
     2941 gtcatattcc ccagcccgcc ataggccaga atgatgccgc tcaccaggtc gttctgttcg
     3001 ttttcctgcg ccacagtgct ttcttcgact tcacaccccg gcgtcagcaa attccagcgg
     3061 cggatggcaa aggtaaacag ggcgtagtac accgctccca tcaccagccc gaccgggaac
     3121 accatgtacc agtgaggcgc gcgcggcagg acgccaaaga acaaatagtc gataagcccg
     3181 ccggagaaag agagcccgat gtgcacgctg aaccactcca tcagcaggaa caccagcccc
     3241 gccaggacgg cgtgaatgcc atatagcacg ggcgcaacaa acaggaagga gaactcaatc
     3301 ggctcggtga tgccgcagac gatggaggtg atcgcgccag agagcataat gcccttcacc
     3361 cgcgctttgt tttccgggct ggcgcagcgg tagatcgcca ccgcaatcgc cggaatgcag
     3421 aacatcttga tcggcgtcag cccggccata aaggttccgg cggtaatcgg cacctgatct
     3481 ttcaactggg caaagaagat aagctggtcg ccatgcacca cctgcccggc tttgttcacg
     3541 tactcgccaa actgcagcca gaacgtcggc caccagacat ggttaagacc aaacggaatc
     3601 agcagccgct cgacaaaacc gaacaggaag gcggacacgc ccggcccctg caccgtcata
     3661 gtattcgaca ggccattaat cagatgctga accggcggcc agaccaccgt catcaccagc
     3721 ccgacaaaca gcgcggcgaa ggaggtcact atcggcacaa atcgcttgcc ggagaagaac
     3781 tccagccatg aaggaagctg gatgcggtaa tagcgtttat acagccaggc ggcgataatc
     3841 ccgataatga tgccgccaaa cacgccggtc tggagcgaag ggatgcccag caccatcgtg
     3901 tagtcacgta cctgcgccac cgactcgggc gtgatcccca gaaactggcc gatggtgacg
     3961 ttcataatca gaaaaccggc gatcgccgac aacccggcaa tcccctgatc gtcactcagt
     4021 cctaccgcca cgccgaccgc aaacagcagc ggcaggttag cgaaaatcgc actgcccgct
     4081 tcagccatca gcttcagcac gtgaaccagc cagtcagcgc cgagaaacgg caggctggcg
     4141 acaatattgg gatcctgaaa gctgacgccg aaggccagca aaataccggc ggcaggcagc
     4201 atggcaatgg ggatcattaa cgatcgcccg atgctttgta acatttgcag aaggttgaat
     4261 cttttcatct cgatctcctg agcgcgtctt tatgacaggg attcgcacaa cagcaggttc
     4321 tgctccgaac accttcagta tccgaaccgg agatgattaa aaaaacagca accgacaacg
     4381 aaagttattt ccggttttcg cttaaaaccg gaaaatttat gaggacaatc acgccggaaa
     4441 ccgtgagaaa gggataaaag aggaaatcgc gttcggtggg aggccatcat atgagtatct
     4501 tcgaggcgca ttttcgcagg ctgcacgccc gctacggcgc aggtcagacg cacgaattac
     4561 agatgcagga gatcgccgcc atcttcggct gttcagtgcg taattgccgg attgctttga
     4621 aaaagatgca tcaggaaaaa tggctcgact ggcagcccca gcgcgggcgt ggcaagcgct
     4681 cacggctcca cctgttaacc tcgccggaaa agctgttcag ccagaacgtc aataagctac
     4741 tggagaagca ggattacggc aacgtgctgc ggtttatcgg caacgacaag tatctgctgg
     4801 atcgcctgag cctgtggcgc tttggggtac aggataaaag cagtgaaacg cgggtgcgca
     4861 tcccctacta tcgcaatctg gatccgctta acccgctcgt ccccctgcgg cggaccgaac
     4921 gacatctcct gcgacagtgc ctcagcgggc tgacgcgcta tgacgccgtt cagggcagga
     4981 tcatccccga tatcgcccac tactggaccc acaacgacga ctttacccgc tgggagttct
     5041 ggctgaaatc caccgcccgc tttgccgatg gcagcgaact gaatgccagc gccgttcagc
     5101 gctgcctgct cgccgccagc cagagcccgc agttcgcgcc gcttttcagc ccaatcaaaa
     5161 ccatcaccgc tgacgccccc tggcatctgg tgattgaaac gtaccatccg gtcagacggc
     5221 tcgactgcct gctcgccact cagccgacga tgctgttcga ttaccagcac ggacacatcc
     5281 gctgtaccgg cgctttccac ctgcaggaac acagtgacaa ctttatggtt ttgcggcgca
     5341 atcaacactg gcaccaggcg aggcccggac tggatgagat caccattttc acctgggctc
     5401 cggagcatat cagcatgagc tttattcccc tgctgcgggg cgaagaggcg caggatgacc
     5461 gcccgctcaa cgagcgtagt ctggagcagg gctgctgttt tgtgctgctc gacggtgaag
     5521 gtgcctttgc ggatgaggcc ggaagacggt ttatcaatta cctgctgcaa cctgtcgaac
     5581 tcctcagcca gacgcagctt cctgacgaat acgcacgcat cctctccgtt gctcagggca
     5641 tgctgccgca gtggaatcac cgcccggtgg atttcggcgg gatcaccgcg ccgtttaacc
     5701 tgcgtcagcc ggttatcatc agcacctttc agcagccgga actggtggag cttgccggag
     5761 cgattcgcat cctgcttgaa cgctggcata ttcgcgccga gatacggatc gacgcatttg
     5821 acagcttcaa cagccagccg cgccctcctg cggatatctg gctcagcaac tttatgctcg
     5881 ataccctttc ggttccggct tttctggaat ggctggcttc caccacgctg tttacgcgac
     5941 tgcctgaaac ccagcgacaa aacctgaacg ccctgctgcc gaccattctg aacagcgata
     6001 atgaacaggc gttcgcaacc attgccgcct ttttccatga gatgacccac cagcgatatg
     6061 tcattccttt gctacatcac tggatggaat ttgcgaccga gaagtcattt acctggcgcg
     6121 atttaaatac gctgggatgg ccggatttta gccaactttg gctcgaataa tgctacgccc
     6181 gcaaaagctc gctaaagggc ctttaaaggc ctttaagggt aagggaggga agtgcgggat
     6241 ttggggcggg gaacggtaga caaattcagt cctcacgacg gagactgttc aaatgtctct
     6301 gtactgacgc cccaatatgg ctttaccacc gtctccgatt gttgaacacg tcaatgtcat
     6361 cgaagatatc tgcacgatcc agatcctggg ttttgtaggc ccgttttctg atgcactgct
     6421 ttttcagcga actgaagcaa ggccatagaa gtaaaacagt accgctggca actgggcatt
     6481 ttctcgcttt agcggagacc aacagtatgc ttagattcag tttatgccgt cagccgatag
     6541 tgcgactgca ccagacccga agggaagctg cggctggata atagcgtcaa atcgatatca
     6601 tggggtaata ctccgaatag cggttttccc gaaccgagca gaacaggaac agttgtgata
     6661 acgatatcgg caaccagacc ttcgcgcagg aacgactgta ttaactgccc gccatcgata
     6721 taaacccggt gcacgttctt ctccataagg tcagcaagag cctcccttgg cgtgcggcat
     6781 gaaaactgta ctttaccctt cagcgccttg ggcacggggg tatcggttag ctgccgggaa
     6841 agcaccagca cgggccgatc ataaggccat tcgtcaaaag tcagcacctt ctcataggtg
     6901 cctcgtccca taatgatcca atctttgtcc gcgatgaatg cggcatagcc atgatcttct
     6961 gtcgggtcat cgcgctttaa cagccagtcg atatcgccat cctgtcgggc gatataacca
     7021 tcaagactga ccgcgatgaa aacatgtgtg gtgaccatca agtgctccgt tttcaggttg
     7081 catatatctc gttgagttta gcaatgttcg ctccaggcac gaagcgagca agttacctaa
     7141 gttgaaggtc cgcagggagc gaggagcaga agttcgaagc acatcatgaa gtaagtttta
     7201 aaatgggaga taacttgctt ttacatattt tccaatgcaa tatcataccc ttttaaaaat
     7261 aaatcagata tatgaggtga aaatgtctag tcttaattta ttcgatgatg tcgtaccttt
     7321 agaaaaacag catccaatat atataatgat gaaaaaggaa cgctatgaac cggagagaga
     7381 agtaattaac caatgggcaa aaggatttct agacagggat aataagttta caaaagaatt
     7441 tcaaacaagt tttgaacctt gcctatggga gttatatctt tttgcttatc ttaaagaatt
     7501 gggtttaagg aacgactttt catacgatgc acctgacttc atcgtcaatg aacctgggtt
     7561 ttgcattgag gcaaccatag cccttccggc acaaggagcc cctggagcgc atggtttcag
     7621 tacggaagat atgccaagag attttaataa gtttaattct gaggcatcta ttcgacttag
     7681 caatagtttt atatcaaagg taaaaaaatt aagatcgaga tattctcaac ttcctcaatg
     7741 taaagaaaaa ccttttgtta tcgccatagc ttccttcgac cgaccttttg cgcactttgc
     7801 cgcagccaga cccatacttg caacattata tggtttatat catgatgaag aagcaaccat
     7861 tgaatcagga tcaaagagca taatttctta taacgttgag gctgcggtaa aaaaagaaaa
     7921 tgttaatata gccatggggt tattttgcac gcctgattat tcggatgtaa gtgctgtgat
     7981 ttatagtagt ttagctactt ggggaaaagt aagagctttg gcggacaatc cttcagctct
     8041 aacaatttat acaaccttta ccccaagaga aaattcatta tatcctaaag ttcatcaagc
     8101 caaaaaaaat gattatatag agcatctggc agatggattg tatattttac ataacccatt
     8161 tgccaagtat ccgttgccta aagagacgct aagccaccct agagtagcac aaggttatgt
     8221 tgaatctgat ggatacgtca attttgtagc gccagaggat tttcttttat tgc
//
LOCUS       DAYPHN010000185         8175 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000185 DAYPHN010000000
VERSION     DAYPHN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.185"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            20..517
                     /gene="ftnA"
                     /locus_tag="ACG0BW_004185"
     CDS             20..517
                     /gene="ftnA"
                     /locus_tag="ACG0BW_004185"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFK7261080.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(555..794)
                     /gene="yecH"
                     /locus_tag="ACG0BW_004186"
     CDS             complement(555..794)
                     /gene="yecH"
                     /locus_tag="ACG0BW_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFK7261081.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            985..2196
                     /gene="tyrP"
                     /locus_tag="ACG0BW_004187"
     CDS             985..2196
                     /gene="tyrP"
                     /locus_tag="ACG0BW_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFK7261082.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVISVQF
                     LIAAGLLPEVG"
     gene            complement(2258..2923)
                     /gene="yecA"
                     /locus_tag="ACG0BW_004188"
     CDS             complement(2258..2923)
                     /gene="yecA"
                     /locus_tag="ACG0BW_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFK7261083.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3119..3205)
                     /locus_tag="ACG0BW_004189"
     tRNA            complement(3119..3205)
                     /locus_tag="ACG0BW_004189"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3169..3171),aa:Leu,seq:taa)
     gene            complement(3218..3291)
                     /locus_tag="ACG0BW_004190"
     tRNA            complement(3218..3291)
                     /locus_tag="ACG0BW_004190"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Cys,seq:gca)
     gene            complement(3346..3421)
                     /locus_tag="ACG0BW_004191"
     tRNA            complement(3346..3421)
                     /locus_tag="ACG0BW_004191"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3386..3388),aa:Gly,seq:gcc)
     gene            complement(3573..4121)
                     /gene="pgsA"
                     /locus_tag="ACG0BW_004192"
     CDS             complement(3573..4121)
                     /gene="pgsA"
                     /locus_tag="ACG0BW_004192"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFK7261084.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4178..6010)
                     /gene="uvrC"
                     /locus_tag="ACG0BW_004193"
     CDS             complement(4178..6010)
                     /gene="uvrC"
                     /locus_tag="ACG0BW_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFK7261085.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6007..6663)
                     /gene="uvrY"
                     /locus_tag="ACG0BW_004194"
     CDS             complement(6007..6663)
                     /gene="uvrY"
                     /locus_tag="ACG0BW_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFK7261086.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6959..7135
                     /gene="yecU"
                     /locus_tag="ACG0BW_004195"
     CDS             6959..7135
                     /gene="yecU"
                     /locus_tag="ACG0BW_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFK7261087.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7122..7346
                     /gene="yecF"
                     /locus_tag="ACG0BW_004196"
     CDS             7122..7346
                     /gene="yecF"
                     /locus_tag="ACG0BW_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFK7261088.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7414..8136)
                     /gene="sdiA"
                     /locus_tag="ACG0BW_004197"
     CDS             complement(7414..8136)
                     /gene="sdiA"
                     /locus_tag="ACG0BW_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFK7261089.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2027 a         2107 c         1910 g         2131 t
ORIGIN      
        1 atctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat
       61 gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca
      121 taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat
      181 gcagcgtctg tttgattatc tgactgatac cggcaattta ccgcgtatta ataccgttga
      241 atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga
      301 acaattaatc acccagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta
      361 cccaacattt aatttcctgc aatggtatgt ttctgagcag catgaagaag agaaactgtt
      421 caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat
      481 cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctcaggcgg caggttttct
      541 gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accgtcttcc
      601 gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc tgtcatccct
      661 tctgccgagc aagtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc
      721 agactggcat gcgtatattg ctcacctgat tcaatcatca tatttaacac ttcatgaccg
      781 tgaatagagt ccatcgttcc tcctcgaaaa aaggctagcg tagcgattgc cgcttaccaa
      841 gactttgcgc cagcgcagga ctgaacgcat tttattgtac atttatattt acaccatatg
      901 taacgccggt ttgacgaacc agccgttatg ccttaacctg cgccgcagat atcactcata
      961 aagatcgtca ggacagaaga aagcgtgaaa aacagaactc tgggaagtgt ttttatcgtg
     1021 gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc aggtgttggt
     1081 tttagcgtca cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta
     1141 ttactgctgg aggtgtacca gcatgtcccg gcagataccg gccttggcac gctggcaaaa
     1201 cgctatctgg gacgctacgg tcagtggctg acgggcttca gtatgatgtt cttaatgtat
     1261 gctctaactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac
     1321 tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt
     1381 ggcgtggttt gtgtcgggac atcgctggtc gatttgttta accgttttct gttcagcgcc
     1441 aaaattattt ttctggtggt gatgctggta ctgctgcttc cgcatattca caaggtgaat
     1501 cttttaaccc tgccgttgca acaggggctg gcactatctg caatcccggt gatttttaca
     1561 tcgtttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg caacattcgt
     1621 aagctacgct gggtgtttat aatcggtagt gcgatccccc tggtggcata tattttctgg
     1681 caggtggcga cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat
     1741 gccgggttaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag
     1801 ctggcggtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta
     1861 ggtttatttg attatctggc agatttgttt cagcgttcaa ataccgttgg tggacgattg
     1921 caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta tccacgagga
     1981 tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct
     2041 tcgctgttga cctggcaaag cagaaagcac aatcctcaag cgggttaccg ggtcaaaggt
     2101 ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattag cgtgcaattt
     2161 ttgattgcag caggcttgtt accagaagtg gggtgatcag atagcctcaa attccttatt
     2221 gggtgccaga attaacgctg acacccaatt tgatctctta atgcaggcag cactgcttaa
     2281 atttcttacc actaccgcac gggcaaggat cattacgccc cggtttctct tctgctttga
     2341 tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt agatcaagcg
     2401 ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatc ttctctaccc
     2461 gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaacgaat
     2521 cgggtaacgt tgaccagtca gaaagcgcca cgccccgcat atagccaaag caccactcct
     2581 caacaatcgt cagctcgctg ccatcgactt ctcgcaagcc gaataacggc tcaaactgct
     2641 ccgggaattc gttcagacgc tctgcggtat cagccatatg ctgaaaagcc aggttcataa
     2701 agcgcgtcat ctctttctct gacgcccagc gtggcacata gtcagcccca ccccacacgg
     2761 caaccagcca ctgtgccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac
     2821 cgtccagctc cgccacatca agaatggcgt ggtcagtgtt gtatttggtc agaatatcgt
     2881 ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga
     2941 tctcaactcg ccagcaccta tcttacatgt cggtccgtaa cagagatact ttttgagtgg
     3001 ctttgccggt gattaaaaat taaggagggt gtaacgacaa gttgcaggca caaaaaaacc
     3061 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg
     3121 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     3181 tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa
     3241 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     3301 aggcctttca gccagacatc cgcttgacgc cgatgtcttt taaactggag cgggaaacga
     3361 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
     3421 cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc
     3481 gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt
     3541 ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat caagcaaatc tgcacgcgca
     3601 gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca
     3661 ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggccaa cgccaccatc
     3721 tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg tttacccaac
     3781 tccgccatcc attcacgtag cgcagaaata ataatttcac gggcgatcat cgttgctgcc
     3841 ggtaaggtca cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg
     3901 agaactttat ctgccacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg
     3961 cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg
     4021 gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg
     4081 acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg
     4141 ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga
     4201 tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctca acgctggcgt
     4261 tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga
     4321 cgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttc ttacggtgcc
     4381 cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc agcgcgggag
     4441 aatctggcgg caaactaaat ccctcacctt ccggctcaaa gaacagcgtt tccagcccag
     4501 ccttcctatc tgcacctttg gcaacgccaa gtagcagcgg atgattttta tcccatgaga
     4561 catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa
     4621 ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga cgcagcacct
     4681 gattcatcgc cgcataatca tcgcccggcg tgatgccagt aatgttatag cgccgatact
     4741 ccgcacgcag cgggccgttg gcatcaaaca ccacacagga agcgacggtt tgttcaccca
     4801 tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc aacacgctgg
     4861 caagcgcggt cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg
     4921 tcgccgcatt ggtgcgcgcg agtttcagat aacgcgcccg atcgccgcga ggtttggttt
     4981 gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc
     5041 taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat
     5101 agaactgacc tacgaaggtt tctaccacct cgctcagttc cgtaccgccc ggcactttcg
     5161 ggaaatagct gcggctgccg agcactttac cctgacgaat gaacaatacg tggacacaag
     5221 ccatgcccgc atcgaacgcc acaccaataa cgtcaaggtc gtcgccggta ttggacacaa
     5281 actgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt
     5341 caaactccag attctggctg gcagtttcca tacggctaat gagttgcgta agcacctgat
     5401 catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac
     5461 tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctgatattgc agacacggac
     5521 gcgagcgatt gcgataaaca ctattttcgc actggcgaat ggggaatatc ttttgcagta
     5581 gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttgg
     5641 catgcttcgc accacgatgc atcgccagac gcggatgggt atcgccactc aggaagataa
     5701 aaggataaga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt
     5761 agttgtgttc cagcaacagc gcttcggttt ctgtatgagt aaccgttaca tcaatttgct
     5821 ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg aaatagctgg
     5881 aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt
     5941 acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact
     6001 gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg
     6061 agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt
     6121 caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttggt
     6181 gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt
     6241 ttctggttcg atctggctta acgccatttg ttgagcgatg tcagaagcaa tgtaacgctg
     6301 ccctgaatag acagaacgga tcgcactcac tacttcctgc ggagccgcgc ctttgctgag
     6361 gtagcccgca gcaccggcct gcatgacttt cgctggtaaa gggttttctg tatggaccgt
     6421 aagcatgatg attttgacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc
     6481 gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg cccggcacca
     6541 cttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct ttatatcttc
     6601 cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat
     6661 caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag
     6721 cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt
     6781 ttgtaaattc gattgtccac agttatggtc acgcccgtca ccaacggctt ccgggatcgt
     6841 ttcacagtaa gcccggcgat gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa
     6901 atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa aaacattcat
     6961 gataaaaata tttattggtc attatattaa cgtattttat agcactgccg atatcacgct
     7021 caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg ccttaacatt
     7081 tttcaccgca aattttcatt gcaacatgac gaggaaaata aatgagtacg cctgattttt
     7141 ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga
     7201 cgctgatgct gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta aagatggaaa
     7261 aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa acggttaagc
     7321 aaattaaaca agcctaccgt cagtaatata aaaccggctg agagcgtgcc tttcagccgg
     7381 tttttgcatc tggcacgcag aacagaaaag agatcaaatt aagccagtag cggccgcgta
     7441 acaggccacc tgggtcttat ttggtgcatt gatttttttc tgcatgtttt tctgatggaa
     7501 attgaccgta ttctcagaga ttgacaaaat catcgctatc tctgctgatg tcttcccttc
     7561 cgccgtccat ttcaaaattt ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac
     7621 tatttcatca tttaaacgca tcagagccat cagactttcg cgcaccagta actgcatttt
     7681 taattgcagt tcatcactaa gaatgggtat ttcgcgcgcg ctgctacggg aaaaggacaa
     7741 aaagcccagc gcccggtttg gcagcattaa atactgagtg acaccgcggc gtaaaccatg
     7801 tgcgcgcgcg gcttcccata acggctgtgc ttcgctgaat aagtcatcat tccacattaa
     7861 atggccctga ctaaagtttt cagggttcag caccggatca attgcgagaa agttttttgc
     7921 ctgataataa ctaacccacg cctcagggta attggtgtag aaagccactt taggtcgcgt
     7981 gaatggtacc gggtggcgaa cacataacga atagtagtcg tactccagct gctgagcctg
     8041 aagctctatt tcatggtaga cctcttctgc ggcctccatc ctctgaaaac gcaacagcat
     8101 cgtgcgacgc cagctgaaaa aatccgtatc ctgcatagta aaccgcaacg cccctgagag
     8161 tgagtatcat ttata
//
LOCUS       DAYPHN010000186         8137 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000186 DAYPHN010000000
VERSION     DAYPHN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.186"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(473..1234)
                     /gene="envY"
                     /locus_tag="ACG0BW_004198"
     CDS             complement(473..1234)
                     /gene="envY"
                     /locus_tag="ACG0BW_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFK7261090.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1417..2307)
                     /locus_tag="ACG0BW_004199"
     CDS             complement(1417..2307)
                     /locus_tag="ACG0BW_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFK7261091.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVNKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2308..5280)
                     /gene="nfrA"
                     /locus_tag="ACG0BW_004200"
     CDS             complement(2308..5280)
                     /gene="nfrA"
                     /locus_tag="ACG0BW_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFK7261092.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRDEKARLQRYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVLALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYVRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5267..7504)
                     /gene="nrfB"
                     /locus_tag="ACG0BW_004201"
     CDS             complement(5267..7504)
                     /gene="nrfB"
                     /locus_tag="ACG0BW_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFK7261093.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1966 a         2034 c         2089 g         2048 t
ORIGIN      
        1 ttttttatat ctaacttaat gagtcaatta catattgctc cactgtttat attttgttta
       61 gtattgaatg attatcacaa tgcgctatct gtttttggtt taattatctg ttattgtttt
      121 atgttgcggt tttactgtgt ggttttttat gcttttgtgg tgtttttatc tatttaagcg
      181 ccatgccttt agaggcatat aagcgaaaat agcatgaggt ttatcctcaa ttgctatgtt
      241 ttttggtaca aaaaagaggg acaaaactga gacacataag gcctcacaat ggcttgcaag
      301 gctttacatg ttttgaggta gtgggacgtg tgagcgcaga gatggcgcgg taagttgttg
      361 acttaaaatg tcgttctagg aacttctaag tcgtgggccg caggttcgaa tcctgcaggg
      421 cgcgccattt cttcttcatt tatccccgtc ttatctgttt ccgctttgcc cttcaccaca
      481 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      541 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgctacc tgagtgatat
      601 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      661 ggctatagct ggtattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      721 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      781 agacgctgtc gcggacgcta ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      841 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      901 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      961 tatgacaagc gataaccggg gtagccagcc gcgaccaggg ctttacgcag gttaagttct
     1021 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1081 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1141 tcggaagggt aaacgtagca tggttattaa cgcttacctc tacctctttt tcggtcagga
     1201 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1261 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1321 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1381 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1441 gacgggcaaa tagcgcagag agaaatagag cgtgtcttta ccgcaagagg aacgtttcgc
     1501 taacagcgag gcaatttctg cgtccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1561 ggcgacaaac agttcataaa caatgccgct tgctggggcg gaactctggc aatcggcgct
     1621 ggcctgtaga taacgttcac gctgttcagc ggtaagttta ttcacgccgc tgccatcctg
     1681 tacccagaca ttaacgccgg ttgctttaac gtgttccagc agttggcgat agccatcggg
     1741 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1801 caaccgctga gcgttgttta accatgtcag caaaggttga cgggcggctt cgctgcgcca
     1861 gttcaggtcg tcaatttccg cgctgatata ccagccatcc ggcgttacgc caggcgcggc
     1921 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1981 cgccgcggat gactgtttct ggtgcataaa aaattccgga tcggcattca gcccgacaat
     2041 cagcttcagg ccagcctgtt gcgcagctgc tgcacgctta aacaataacg cgcgctgttc
     2101 tggctgggtg aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2161 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatcacggtt
     2221 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2281 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2341 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatatt cgacgccgag
     2401 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2461 cccgacaccg ccaagctgcg cgccctgagt gcctttatca cgataaccgt tgtcctgaac
     2521 gtgagcgtaa ggctcaatag tctgcccgtt cgccaccttc tgatgccagc tgacgcgata
     2581 atctgccgtc cacgcctgaa tatcctgacg gacatattgc gccgcatcga ggtacaggtt
     2641 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2701 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2761 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2821 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2881 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2941 ggcttccagt tgcccatagc tacgatagct tttccctggc gctgcgccgc cgacattatt
     3001 gttagcggta ctcattgcgc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3061 gcggcgaccg acctcctcat gcagacggcg gaaattgaag cgttgctgat tttgctctgg
     3121 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3181 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggataagtgc
     3241 cggatcgtcc ggtagccctt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3301 accgctatcc cacaaggcgt aaccaagcgc tgcctgggtg ttgctattat tcggttccag
     3361 ttccagcgcg gcgcgcaaat cactcaccgc cgccgggaca ttatgacgtt ggcgataaat
     3421 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3481 gttcagtgcg agttccggct gaccaggaat gtaacgttgc gcatgcagcc accagtagag
     3541 ggcattgttt cccagcccac gttgttctgc ctgctgaagc cagcgatcgc gagccgcacc
     3601 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3661 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3721 ataggctacc gcacgatgtt gccaggcgtt cggttgtcgt tgttcggcct gaagccatgc
     3781 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3841 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3901 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3961 aatcgccacc ttcgccggag ttgccaggta aggatgactt tccagcagag acgccagtcg
     4021 cgccattaac gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4081 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4141 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag cttcagcctt
     4201 gttacgggtc gccacgctaa cggtataacg ctcctcaagc atttcattgg cggggagggt
     4261 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4321 gccaacaaca tactggcggt tatcggcaaa ctgtaccgta taattcgcca acgccagaac
     4381 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg catccgtggt
     4441 aaacagcggc ttattttcaa tgagataacg ctggaggcgt gctttttcgt cacgataagc
     4501 cagcgcggtc gcgtaagtaa tatatgactg cggatcggtg aagatcccct gtgattgcag
     4561 cgccaggatc cgatcgtcca gctgcccggc aagaagcaca tcaaaccact ggcggcgttc
     4621 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4681 ttgcttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4741 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaattgtg ccagccgcag
     4801 ggcattctgc ccaacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4861 ctgggcaagc agttcttcaa cagtcgtaac gcttttcact tcaactggaa tagccgccag
     4921 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4981 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag ttagcggaat
     5041 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5101 atttttctgt gccttcagcg ccttgtcgag acggggataa ataacaaaat ggcgataatc
     5161 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5221 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5281 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5341 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5401 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5461 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5521 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5581 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgcgcatacc
     5641 agtggcgtaa ccccgtgaca atttgtcccc gcagaacaat gacgtaacgc actttgcgtc
     5701 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5761 ttaactcatc attttccaga cgcagcggca gcactgcata atgcagcgcc acggaggccg
     5821 gcatttcggc aatcagtgat gaagggatct gccaggcgtc gatcgactcc catgccacgc
     5881 cgttttgctc tgccagcgcc tgtgccagct gttcggcgct aatcagcccc tgcatcagca
     5941 ttgaaccgcc caggcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttctt
     6001 cagtgatgac ctgattttcc agcaggattt gacctaacgg acgcaacgag cgggtatcgc
     6061 cagtcacgct ggggaagtca tgcgttgttt tatcccacgc gacacgacgt ggatcgccgt
     6121 gttgaagtac ctgttttaac gcgcgccagt tggccatgaa gttaatcagg ttgccccaga
     6181 aaagacgcag gacagatagc agcccctgcg tcaggccgta gtagccagta acgaaaatca
     6241 cccgctgcac gatgcggtta atcatcaagc caaagtttag ccacagcaga gtcattaacc
     6301 atgcgctgcc gctgaaaata gaaaggaaat gccaggcatc gggccacaaa ctttcatacg
     6361 ccagcaacag caaaagctgg atcatcacca gcatcgcgag gaagctgaca aagttactga
     6421 ttgccccttt gcggtcgcgc cagagaaagt agttcagcgt cagactggag gtccatttgt
     6481 gggttttaaa gccctggaaa acaatgccga tgatccagcg agatttttgt cgaaccgcag
     6541 tcgaaaaggt atcggggaaa tattcgcgca cgcagatcat gtttgacgtc cgcgcgtgct
     6601 gtaaaaattt acgctgctcg cgttctttgg cttcgtccac caccggaaaa cggacaaaaa
     6661 tttccgtcat acctttttct ttcaggcgga aaccaatgtc gtaatcttca gtaagactct
     6721 gcacgtcgaa agcaatacca tcaccgtcag ctaacagtgc ggtcacggcg cggcggctga
     6781 aacaagtacc gacgcctgcg ctgggcacct gtccggcgag ggcttcacgc accggaacat
     6841 ctttgccatg cagttctgaa aactcatcaa tgtaagtcat gctggtgaag tgcgtccatt
     6901 cgcgttcgaa tgggtatacc gggatctgaa tcagatcttt acgctcgacc agatagttga
     6961 acagacgcaa ttccatcggt gaaattacgt cttcggcgtc atgcagaata aaaccagcaa
     7021 aagcgaaatt ggcgctacgc tcaaactggg tgatggcgtc cagcacgttg ttcaggcagt
     7081 cggctttgct ggtggggcca ggacgcgcgc agactacctt atgcacgttt gggaagcgag
     7141 cgcacacttc gtcaacatca cgctgagtat cggggtcgtt ggggtaggtg ccaacaaaga
     7201 tatgatagtt ttcatagtcg agcgtggtcg ccgccagctc ggccatattg ccgatgacgc
     7261 ccgtttcatt ccacgccgga accataatcg ctaacggttt ttcatctggt ttatacagtt
     7321 cgcggtaact cattcgcggg tagcggcgat aaacactcaa cttgcgttta atgcggcgta
     7381 cccagtagac gacatcaata aaaaaatcgt ccagcccgct gatgaacatg atgaccgcta
     7441 acgttatcgc gattactttt aagccgtaga gccaggtagc aaaaacatca agaagccagt
     7501 ccacacaaaa accttacatt aacgctggtt atgtttaggg tggcgtatat taaggttttt
     7561 tatgaattgt gacagctttt taccattaat aggtatgact attgcggcac gttattttta
     7621 cactggttat aaaagttgcc gtttgcgtag aatgtagaca aaagcgtggt gtcaaggagg
     7681 ggcggtaaac gtcatagtga agaggagtgg tggcaccgat caggtcatca tgtagtgtaa
     7741 gcgtctggag gaggtgagca ggggagatca ttttccccgt gctgaacaaa aagacggcgt
     7801 tgctgtgggt acaggaaggc taacttcatt tctctttaga ttatttaagt ttaatattca
     7861 accaggaaat aaagaacgtt aaaataacaa aggaaaaaac aatcaaagag acattcctga
     7921 aaaatacgtg ctcataaaaa agataaaaaa cgtacaaaat tgggcacgca actttcagga
     7981 cgtttatatg tttagaattc tctttatctt taaagttaaa attacataaa aatataaaga
     8041 aatatgaaag catggctgaa aacatcattg ataaggcgcg atatggcagt cctgaattaa
     8101 aggtttcgac tcctaataat attgtcgata gcgttgc
//
LOCUS       DAYPHN010000187         8126 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000187 DAYPHN010000000
VERSION     DAYPHN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.187"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            234..1217
                     /locus_tag="ACG0BW_004202"
     CDS             234..1217
                     /locus_tag="ACG0BW_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFK7261094.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1289..2437
                     /locus_tag="ACG0BW_004203"
     CDS             1289..2437
                     /locus_tag="ACG0BW_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HFK7261095.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2461..4137
                     /locus_tag="ACG0BW_004204"
     CDS             2461..4137
                     /locus_tag="ACG0BW_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFK7261096.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKIAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRSSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4147..4887
                     /gene="kdsB"
                     /locus_tag="ACG0BW_004205"
     CDS             4147..4887
                     /gene="kdsB"
                     /locus_tag="ACG0BW_004205"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFK7261097.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAAEAAEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4884..6911
                     /locus_tag="ACG0BW_004206"
     CDS             4884..6911
                     /locus_tag="ACG0BW_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFK7261098.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFAALRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAAWGLTVDEHHCPRRERRLTIADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYCRKLIMFCKVRFG"
     gene            6946..8115
                     /locus_tag="ACG0BW_004207"
     CDS             6946..8115
                     /locus_tag="ACG0BW_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFK7261099.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWHEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDIKLFKKFRGYLLVKTQVNAVYY"
BASE COUNT         2084 a         1968 c         2106 g         1968 t
ORIGIN      
        1 taggtgggca aaatttggtt ccctttctcg cctgtaatat tgcagcgtct tcgcacctcc
       61 atgagacatt gcgacttaat aagaaggtga taagtcctgc atataagcat ggactgacca
      121 tggttttata ttcattaaaa attttgttac aacccattga tttagcataa ataaattata
      181 gtgggttcgg gtttgttgtg actgtggcat tatttccgtg caaaggagct gatatgtctg
      241 aaagacattt acctgatgac cagagcagta ctatcgatcc atatctaatt acctctgttc
      301 gccagactct ggcagaacaa agcgcagcat tacaaaactt gtctaaacaa ctggattccg
      361 ggcagtacca gcgtgtcctt aatttgataa tgaactgtaa agggcacgtt attctttcgg
      421 gaatgggtaa atcagggcat gtcggtcgta aaatgtcagc gacgctggcc tctacgggta
      481 cgcctagttt ctttattcat cctgcagaag ctttccatgg cgatctgggc atgattacgc
      541 cttacgatct tctgatcctt atttctgcca gcggtgaaac ggatgaaatc ctcaagctag
      601 ttccttcact gaaaaatttc ggcaaccgaa ttatcgccat taccaataat ggaaattcca
      661 cgctggcgaa aaatgctgat gccgtgctgg aactccacat ggcgaatgaa acctgcccaa
      721 ataatcttgc accaacaacg tctaccacgc tgacgatggc gatcggcgat gcgctggcga
      781 ttgccatgat ccaccaacgc aaatttatgc cgaatgattt tgcgcgctat cacccgggcg
      841 gttcattagg tcgtcgcctg ctgacccgcg ttgctgatgt catgcagcat gatgttcctg
      901 cggtacagct ggatgcgtca tttaaaaccg tgattcaacg tatcaccagc ggatgccagg
      961 gaatggtgat ggtagaagac gcagaaggtg ggctagcggg cattatcacc gacggtgacc
     1021 tgcgtcgctt tatggaaaaa gaggattctc tgacatccgc cacggctgcg cagatgatga
     1081 cacgtgaacc gctgacgcta ccggaagaca ccatgatcat tgaagcggaa gaaaaaatgc
     1141 aaaagcaccg cgtctcaacg ttattggtga ccaacaaggc aaataaagtc actggccttg
     1201 tgcgcatttt cgactaatta agcaacgggg tgcgagaggt tagtctctca ttgagtttgc
     1261 gccccactga aactttgata atcgttacat gttgataaaa gtgaagtctg ccgtatcctg
     1321 gatgcgtgct cgtctgtctg ccatctcact ggcagatatc caaaaacacc tggcgaaaat
     1381 catcattctg gcaccgatgg cggtgctgct gatctatctg gctatcttca gccagcctcg
     1441 ctatatgagc gagtcgaaag tcgccattaa acgctcggat gatttaaaca gcggcagcct
     1501 gaattttggt ctgcttctgg gtgcctctaa ccccagttcc gcagaagatg cgttgtatct
     1561 gaaagagtac atcaactcgc cggatatgct ggcggcgctg gataagcaac taaattttcg
     1621 tgaagcgttt agccacagcg ggctcgattt tcttaatcat cttagcaagg atgaaaccgc
     1681 agaaggcttc ctgaagtact acaaggaccg tatcaacgtc tcgtatgacg ataaaaccgg
     1741 attactgaat attcagacgc agggctttag cccggagttt gcgcttaagt ttaaccaaac
     1801 cgtgctgaaa gagtcagagc gctttatcaa tgagatgtca catcgcatcg cgcgtgacca
     1861 gcttgccttt gcagaaacgg agatggaaaa ggcacgccag cgtctggacg ccagcaaagc
     1921 ggaattgctc tcttatcagg acaacaacaa cgttctggat ccacaggcac aggcacaggc
     1981 ggcgagcacg ttagtgaata cgctgatggg ccagaagatc cagatggaag cggacctgcg
     2041 gaacttgctg acgtatctgc gtgaggacgc cccgcaagtt gtgagtgcgc gtaatgcgat
     2101 tcagtcattg caggcacaaa ttgacgaaga aaaaagcaaa atcactgcgc cacagggtga
     2161 caagctaaac cgtatggcag tggattttga agaaatcaaa tcaaaagtag agttcaacac
     2221 cgagctgtac aaactgaccc tgacctccat tgaaaagacc cgtgtagaag cggctcgtaa
     2281 gctcaaggtg ctgtcagtga tcagttcgcc acagttgccg caggaatcgt cttttccaaa
     2341 tatcccttat ttgatcgcct gctggttact ggtgtgctgc ctgctgttcg gcaccctgaa
     2401 actgttgctg gctgttattg aagatcaccg agactaacgc tgtcgctgaa tgagtttgtg
     2461 atgaaattat ttaaatcaat tttactgatt gccgcctgtc acgcggcgca ggccagcgcg
     2521 accattgata ttaacgctga cccaaacctg acaggagccg cgccgcttac cggtattctg
     2581 aacggacaga agtcggatac gcaaaacatg agcggcttcg acaatacccc gccgcccgca
     2641 ccgccggtgg taatgagccg tatgtttggt gctcaacttt tcaacggcac cagcgcggat
     2701 agcggtgcga cggtaggatt caaccctgac tatattctga atccgggtga tagcattcag
     2761 gttcgcttgt ggggtgcgtt cacctttgat ggtgcgttac aggttgatcc caaaggtaat
     2821 attttcctgc cgaacgttgg tccggtgaaa attgctggcg tcagtaatag tcagctaaat
     2881 gccttggtca catccaaagt gaaggaagta taccagtcca acgtcaacgt ctacgcctcc
     2941 ttattacagg cgcagccagt aaaagtgtac gtgaccggat ttgtgcgtaa tcctggtctg
     3001 tatggcggtg tgacgtctga ttcgttactc aattatctga tcaaggctgg cggcgttgat
     3061 ccagagcgcg gaagttacgt tgatattgtg gtcaagcgcg gtaaccgcgt gcgctccaac
     3121 gtcaacctgt acgacttcct gctgaacggc aaactgggac tttcgcagtt cgccgatggt
     3181 gacaccatca tcgtcgggcc acgtcagcat actttcagcg ttcagggcga tgtctttaac
     3241 agctacgact ttgagttccg cgaaagcagc attcccgtaa cggaagcgtt gagctgggcg
     3301 cgccctaagc ctggcgcgac tcacattacg attatgcgta aacaggggct gcaaaaacgc
     3361 agcgaatact atccgatcag ttctgcgcca ggccgtatgt tgcaaaatgg cgatacctta
     3421 atcgtgagca ctgaccgcta tgccggcacc attcaggtgc gggttgaagg cgcacactcc
     3481 ggtgaacatg ccatggtatt gccttatggt tccactatgc gtgcggttct ggaaaaagtc
     3541 cgcccgaaca gcatgtcgca gatgaacgca gttcagcttt atcgctcatc agtggctcag
     3601 cgtcagaaag agatgctgaa tctctcgctg caaaagctgg aggaagcctc actttctgcc
     3661 cagtcatcca ccaaagaaga agccagcctg cgaatgcagg aagcacaact gatcagccgc
     3721 tttgtggcga aagcacgcac cgtggttccg aaaggtgaag tgatcctcaa cgaatccaat
     3781 attgattctg ttctgcttga agatggcgac gtcatcaata ttccggagaa aacatcgctg
     3841 gttatggttc atggcgaggt gctgttcccg aacgcggtga gctggcagaa agggatgacc
     3901 accgaggatt acatcgagaa atgcggtggc ctgacgcaaa aatcgggtaa cgccagaatt
     3961 atcgtcattc gtcagaacgg tgcggcagtc aacgctgaag atgtagattc actcaagcct
     4021 ggcgatgaga ttatggttct gccgaaatat gaatcgaaaa acattgaagt tacccgtggt
     4081 atttccacca tcctctatca gctggcggtg ggtgcaaaag tgattctgtc tttgtaaggg
     4141 gttgaaatga gcaaagcagt tattgtcatt ccggctcgct atggctcctc gcgcctgccg
     4201 ggtaagccac tgctcgatat tgttggtaaa ccgatgatcc agcatgttta cgagcgtgcg
     4261 ttacaggtgg cgggcgttgc ggaagtttgg gtggcaacag acgatccgcg tgttgaacag
     4321 gccgtacagg cgtttggcgg gaaagccatc atgacgcgca acgatcatga atccggcacc
     4381 gatcggctgg tcgaggtgat gcataaagtc gaggcagata tctacattaa cctgcagggc
     4441 gacgaaccaa tgattcgccc gcgggatgta gaaacgctgc tacaaggaat gcgtgacgat
     4501 cccgcgttgc cggtggcaac gctatgccac gcgatttctg ccgcagaagc ggctgagcca
     4561 agcacggtaa aagtggttgt gaatacccgc caggatgcgc tttatttcag ccgctcaccg
     4621 attccgtatc cgcgtaatgc tgaaaaagcg cgctacctga aacacgtcgg tatttacgct
     4681 tatcgtcgcg atgtgctgca aaactacagc cagttaccgg agtccatgcc ggagcaggcg
     4741 gaatcactgg agcagctaag gttgatgaac gcggggatca acatccgcac atttgaggtt
     4801 gccgcaaccg gtccgggcgt cgacacccca gcatgcctgg aaaaagtgcg cgccctgatg
     4861 gcacaggaac tggctgaaaa cgcatgattg gcatttactc gcctggcatc tggcgtattc
     4921 cgcatctgga gaaatttctg gcgcaaccgt gccagaaact ttctctgctg cgccctgttc
     4981 cacaagaagt tgatgctatc gccgtgtggg gacatcgtcc cagcgcggcg aaaccagtcg
     5041 ccatcgccaa agcagcggga aaacccgtca ttcgtctgga agatggattt gtgcgttcgc
     5101 tggatcttgg cgtcaatggc gagccgccgc tttctctggt ggtggatgat tgttgcattt
     5161 actacgatgc cagcaagcct tcagcactgg agaaactggt acaggataaa gccggaaata
     5221 cagctctgat aagccaggcc agagaagcga tgcacaccat cgtgaccggg gatttgtcga
     5281 aatataacct ggcacctgcg tttgtggctg atgagtcaga acgttcagac atcgttctgg
     5341 ttgtcgatca gacatttaat gatatgtcag tgacgtatgg caatgctggc ccgcatgagt
     5401 ttgctgccat gctggaagcc gcgatggcgg aaaatcctca agccgaaatt tgggtgaagg
     5461 tgcatccgga tgtcctggaa ggaaagaaaa caggttattt cgccgctctg cgcgccacgc
     5521 aacgagtacg tttaattgcc gagaatgtca gcccgcagtc gctgttgcga cacgtttccc
     5581 gggtttacgt cgtgacctcc cagtacggct ttgaagcctt gctggcagga aaaccggtga
     5641 catgcttcgg ccagccctgg tatgcaggct ggggcttaac cgacgatcgc catccgcagt
     5701 ccgctttgtt atctgcccga cgcggttctg ccacgctgga ggaacttttt gccgctgcat
     5761 acctgcgtta ctgtcgctat atcgatccgc aaacgggaga agtaagcaat ctatttaccg
     5821 tgctgcaatg gctgcaatta caacgtcgac atctgcaaca gcgtaatggt tatttatggg
     5881 cgccaggctt aacgctgtgg aagtcagcga tcctgaaacc tttcttgcaa acggcaacaa
     5941 accggctgag tttttcacgt cgctgtactg cggcgagcgc ctgcgtggta tggggtgtaa
     6001 agggagaaca gcaatggcga gccgaagcgc agcgaaaatc actgccgtta tggcgaatgg
     6061 aagatggttt tctacgttca tccggacttg gctctgacct tttgccgccg ctatcgttgg
     6121 tcctggataa acgcgggatc tactatgacg ccacgcgccc cagcgacctg gaagtgctgc
     6181 ttaatcacag ccagttaaca ctggcgcagc agatgcgagc tgaaaaatta cgccagcgac
     6241 tggttgaaag caaactgagc aaatacaacc tgggggccga tttctctctg cctgccgagg
     6301 ccaaagataa aaaaatcatc ctggtgccgg gtcaggtaga agacgatgcc tcaattaaaa
     6361 caggcactgt gtcgattaag agcaaccttg agttattacg cacagtacgc gagcgcaatc
     6421 cgcacgctta cattatttat aaaccgcacc cggatgtact ggtggggaat cgcaagggca
     6481 atattccgac agaactaatt gctgaactcg ctgattatca ggcactggac gcagatatta
     6541 ttcaatgcat tcagcgcgca gatgaagtgc acaccatgac atcattgtcc gggtttgaag
     6601 cgttattaca tggcaagcaa gttcattgtt acggcctgcc cttctatgcc gcttggggtt
     6661 taaccgttga tgaacatcat tgcccgcgcc gcgagcgcag attaacgata gcagacttga
     6721 tctatcaggc gttgattgtt tacccaacat atattcatcc aacacggcta cagcctatta
     6781 cggttgagga agctgcggaa tatttaatcc agacgccgcg taagccgatg tttattaccc
     6841 gaaaaaaagc ggggcgggta atacgctatt gccgcaaatt aattatgttc tgcaaggtca
     6901 gatttggcta aacaatttca tagttgacta ttacgcaaat tatttatgca aggtaatgca
     6961 ctaaccgttt tattatccgg taaaaaatat ctgctattgc aggggccgat gggacctttt
     7021 ttcaatgacg tcgccgaatg gttagagtca ttaggacgta acgctgtgaa tgttgtattc
     7081 aacggtgggg atcgttttta ctgccgccat cgacaatacc tggcctacta ccaaacgccg
     7141 aaagagttcc ccggatggtt acgggatctc caccggcaat atgactttga taccatcctc
     7201 tgctttggtg actgccgccc attgcacaaa gaagcaaaac gttgggcaaa gtcgaaaggg
     7261 atccgctttc tggcatttga ggaaggatat ttacgcccgc aatttattac cgttgaagaa
     7321 ggcggagtaa acgcatattc atcgctaccg cgcgatccgg atttttatcg taagttacca
     7381 gatatgcctg cgccgcacgt tgagaactta aaaccttcaa cgatgaaacg tataggtcat
     7441 gcgatgtggt attacctgat gggctggcat taccgccatg agttccctcg ctaccgccac
     7501 cataaatcgt tttccccctg gcatgaagca cgttgctggg ttcgtgcata ctggcgcaag
     7561 caactttaca aggtaacaca gcgtaaggta ttaccgaggt taatgaatga gctggaccag
     7621 cgttattatc ttgccgtttt gcaggtgtat aacgatagcc agattcgtaa ccacagcaat
     7681 tataacgatg tgcgtgacta tattaatgaa gtcatgtact cattttcacg taaagcaccg
     7741 aaagaaagtt atttggtgat caagcaccat ccaatggatc gtggtcacag actctatcga
     7801 ccattaatta agcggttgag taaggaatat ggcttaggcg agcgcgtcat ttatgtgcac
     7861 gatctcccga tgccggaact attacgccac gcaaaagcgg tggtgacaat taacagtacg
     7921 gcggggatct ctgcgctgat tcataacaaa ccactcaaag tgatgggcaa tgccctgtac
     7981 gacatcaaag gattgacgta tcaagggcat ttgcaccagt tctggcaggc cgattttaaa
     8041 ccagatataa aactgtttaa gaagtttcgt gggtatttat tggtgaagac gcaggttaat
     8101 gcggtttatt attaacatct tatcaa
//
LOCUS       DAYPHN010000188         8080 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000188 DAYPHN010000000
VERSION     DAYPHN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.188"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..113
                     /locus_tag="ACG0BW_004208"
     CDS             <1..113
                     /locus_tag="ACG0BW_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261100.1"
                     /translation="YYNGRVYKKSNFAGDCETAFALAVKFAPTQLLQLLL"
     gene            complement(158..2203)
                     /gene="dcp"
                     /locus_tag="ACG0BW_004209"
     CDS             complement(158..2203)
                     /gene="dcp"
                     /locus_tag="ACG0BW_004209"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFK7261101.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2340..3086
                     /gene="ydfG"
                     /locus_tag="ACG0BW_004210"
     CDS             2340..3086
                     /gene="ydfG"
                     /locus_tag="ACG0BW_004210"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFK7261102.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3175..3861
                     /gene="rspR"
                     /locus_tag="ACG0BW_004211"
                     /gene_synonym="ydfH"
     CDS             3175..3861
                     /gene="rspR"
                     /locus_tag="ACG0BW_004211"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFK7261103.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4038..4241
                     /gene="ydfZ"
                     /locus_tag="ACG0BW_004212"
     CDS             4038..4241
                     /gene="ydfZ"
                     /locus_tag="ACG0BW_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFK7261104.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4277..5737)
                     /gene="ydfI"
                     /locus_tag="ACG0BW_004213"
     CDS             complement(4277..5737)
                     /gene="ydfI"
                     /locus_tag="ACG0BW_004213"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFK7261105.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATKHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5827..7110)
                     /gene="ydfJ"
                     /locus_tag="ACG0BW_004214"
                     /pseudo
     CDS             complement(5827..7110)
                     /gene="ydfJ"
                     /locus_tag="ACG0BW_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            7715..7828
                     /gene="ynfT"
                     /locus_tag="ACG0BW_004215"
     CDS             7715..7828
                     /gene="ynfT"
                     /locus_tag="ACG0BW_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFK7261106.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            7897..>8080
                     /gene="ydfK"
                     /locus_tag="ACG0BW_004216"
     CDS             7897..>8080
                     /gene="ydfK"
                     /locus_tag="ACG0BW_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFK7261107.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQ"
BASE COUNT         2176 a         2037 c         1816 g         2051 t
ORIGIN      
        1 attattataa cggaagggta tacaaaaaaa gtaattttgc tggcgactgc gaaacggcat
       61 tcgcattagc ggtgaagttt gcccccaccc agctgctgca attactgctc taaccaggct
      121 ggcctggcga tatctccagg ccagccattg gtggtgttta tatgttcaag ccacgatgtt
      181 gcagcatcgg cataatctga ggtgccttac cgcgccattg tcgatacagg cgttccagat
      241 cttcgctgtt acctctggaa agaatcgcct cgcgaaaacg ccgcccattt tcacgcgtta
      301 atccgccctg ctcaacaaac cactggtaac catcatcggc caacatttgc gtccacagat
      361 aagcgtaata acctgcggca tatccgccac caaaaatatg ggcgaaataa ctgctgcgat
      421 agcgtggcgg tatagcggga agatccatat tttctgccac cagcgcccgc aattcgaaat
      481 catcgacatc ctgcattgct tcgttttctt ccaggcaatg ccagcgcata tcgagaagtg
      541 cggcgctaag cagttcgctc atctcatacc ctttattgaa caggctggca ttacgcattt
      601 tctgttgcag ttcgtcaggc attgctgccc cgctctgata atgccgggcg tagcgagcga
      661 atacctgcgg atgcgttgcc cagtgttcgt tgatttgcga cggaaattcg acaaaatcac
      721 gcggcgtgtt ggtgccggaa agtgtggcat aacgctggcg ggcaaagagg ccgtgcaacg
      781 tatgaccaaa ttcatggaat aaggttatga catcatccca gagtaacaac gcaggctcac
      841 cggcagcggg tttctgataa ttgcagacgt tataaattac cggatgcgtt tcattaagcg
      901 ttgattgctc aacaaaattg cccatccatg caccgccgct ttttgaatca cgggcgaaga
      961 aatcaccgta aaataacgcc agccccacgc cattatgatc aaaaatttcc catacacgaa
     1021 cgtcaggatg gtagacagga atatcaaaac gttcgacaaa cttaataccg aagagctgat
     1081 tcgcggtcca gaatacacct tcattcaaca ccgtgtttaa ttcaaaatat ggcttgagct
     1141 gcgattcatc gagatcgaat ttctcacgtc ttacctgctc ggcataaaat gcccagtccc
     1201 acggctgcgc gctaaacccg ccctgctgct tatcgataac cgcctgtatg gaggctaatt
     1261 catcgctcgc acgttgacgc gccgctggaa caatttcccg cataaagttg agtgctgctt
     1321 ctggcgtttt tgccatctga tcggcgattt tccatgcggc ataatgaggg aaaccaagca
     1381 gtttcgcctg ctgcgcgcgg atctccacca gacgttgaat gatagcgcgg gtatcattgg
     1441 catcattttt ttccgctcgc gtccagcccg cagtgaacag tttttcacgc gtcgcgcgat
     1501 cgcgcagttc agcaagcgct ggttgctggg tggtattcag caacggaatc agccatttgt
     1561 tatccagacc tttctcgcga gccgcctctg ccgccagcgc aatctcttgc tcactcattc
     1621 ctgccagctg cgcgaaatcg ttcacaacca gaccgccgga tttatttgct gccagtaacc
     1681 gctggttaaa ctggctggtc agggtcgcag cttctgtatt caggactttt aattttgctt
     1741 tatcagcttg tgcaagtttg gctccggcaa ggacaaaacg ttgatgaatg acctccacca
     1801 ggcggatgga ttcactatca agtcccaggg attcacggcg ttgccagaca gcatctaccc
     1861 gcgcgaataa ttcaccgttc agatagatat cattagccag ttccgccagt tcagcggaaa
     1921 actgttcatc aagacgctgt aattcatcat tagtatgcgc cgcagtcatc gcaaaaaaga
     1981 cgctggtaac gcgggtaagt aattctccgc tttgttccag tgccagaata gtattgttga
     2041 aatcaggtgt ttgcgggtta agcgcgatgg cagcaatttc tgcccgcttt tgctgcattc
     2101 cctcatcgaa tgccgggcga tagtgatgat tggcaatttg atcaaaatgg ggagccagat
     2161 acggcagtgt gctttgcaca aggaaaggat tcattgttgt cattttcttc tcctgaacgc
     2221 gaggtgttcc atagcgtagg cttactgata acggagtgca atcttgcaaa ccagtattac
     2281 ccgctcttaa gcatcacgtg ctatgttatt gacacacaaa agcgttgagg aacagtgaga
     2341 tgatcgtttt agtaactgga gcaacggcag gttttggtga atgcattact cgtcgtttta
     2401 ttcaacaagg gcataaagtt atcgccactg gccgtcgcca ggagcggttg caggagttaa
     2461 aagacgaact gggagataat ctttatatcg cccaactgga cgttcgcaac cgcgccgcta
     2521 ttgaagagat gctggcatcg cttcctgccg agtggagcaa tattgatatc ctggtaaata
     2581 atgccggcct ggcgttgggc atggagcctg cgcataaagc cagcgttgaa gactgggaaa
     2641 cgatgattga taccaacaat aaaggcctgg tatatatgac gcgcgccgtc ttaccgggta
     2701 tggttgaacg taatcatggt catattatta acattggctc aacggcaggt agctggccgt
     2761 atgccggtgg taacgtttac ggtgcgacga aagcgtttgt tcgtcagttt agcctgaatc
     2821 tgcgtacgga cctgcatggt acggcggtgc gcgtcaccga catcgaaccg ggtctggtgg
     2881 gtggcaccga gttttccaat gtccgcttta aaggcgatga cggtaaagcg gaaaaaacct
     2941 atcaaaatac cgttgcattg acgccagaag atgtcagcga agccgtctgg tgggtatcaa
     3001 cgctgcctgc tcacgttaat atcaataccc tggaaatgat gccggttacc caaagctatg
     3061 ccggactgaa tgtccaccgt cagtaatttt tatacccggc gtaactgccg ggttattgct
     3121 tgtcacaaaa aagtggtaga ctcatgcagt taactcactc acaagcaaga acgaatgacc
     3181 gtcgaaacgc aacttaatcc cacacagcct gtcaatcagc agatttatcg tattcttcgt
     3241 cgcgacattg tccattgcct gattgcacca ggcacaccgt tgtcggaaaa agaagtttct
     3301 gttcgtttca atgtgtcacg ccagccggtt cgtgaagcct ttattaaact ggcggaaaac
     3361 ggcctgattc aaattcgtcc gcagcgtggc agctacgtca acaaaatttc catggcccag
     3421 gtgcgcaacg gcagttttat ccgtcaggcc attgagtgcg cggtggcgcg tcgggcggcg
     3481 agcatgatta ccgaaagcca gtgctaccaa ctggaacaaa atcttcacca gcaacgcatt
     3541 gccattgagc gcaagcaact ggatgatttt tttgaacttg atgacaactt ccatcaactc
     3601 ctgacgcaga ttgccgactg tcaactggcg tgggatacca ttgagaacct gaaagcgacc
     3661 gttgatcgcg tgcgctatat gagtttcgac cacgtttctc caccagaaat gctgttacgc
     3721 cagcatcttg agattttctc tgccctgcaa aaacgtgatg gcgatgcggt agaacgtgca
     3781 atgacgcaac atttgcagga aatcagcgaa tccgtgcgcc agatccgcca ggaaaacagc
     3841 gactggttta gcgaagagta attcatttcc tctcatccca ttcggggtga gagtctctcc
     3901 ccccgcctta tggctcatgc atgcatcaaa aaagatgtga gcttgatcaa aaacaaaaaa
     3961 tatttcactc gacaggagta tttatattgc gcccgttacg tgggcttcga ctgtaaatca
     4021 gaaaggagaa aacacctatg acgacctacg atcgtaaccg taacgcaatc accactggca
     4081 gccgtgttat ggttagcggc accggtcaca ctggcaagat cctgtcgatt gatactgaag
     4141 gtctgaccgc tgagcaaatc cgccgcggaa aaaccgtagt tgttgaaggt tgtgaagaga
     4201 aactggcacc tctggacctg attcgtctcg gcatgaacta agcgtgtgaa tgccgccgat
     4261 ggcggcattg ctttttttac ttcacggaat atttcgcaac ggtcgctttc gcaccatgcg
     4321 ctaataaaga caagtacgct tccgtcactt ttgcagtaaa caagctattg tctggcaaat
     4381 catcaccaaa gatcgcctta atcgccagca atgactggac gcgcgctttc ccttcggcac
     4441 tactttgtac agccttctga ataacaggta acagtgggtc actgatttct atcggatttc
     4501 cctgttcatc aacaccaccg acataacgca tccaacctgc gacgcccagc gccagcagat
     4561 cgaacttgct gtcatgcgcc agatgccagc gaacagaatc caacatccgc tgtggcaatt
     4621 tctggctgcc atccatcgca atctgccagg ttcgatgacg tagcgccggg ttgctatagc
     4681 gttcaattaa tcggttagcg taatcttgca aatcaacgcc ctgcactttc aacgtcggcg
     4741 cttgttcctg caacatcaag gcatacgctg catgacgata atgttcatct tccatgcagt
     4801 cattaatgtg ctgatatccg gcaagatacc ccagatacgc caggaatgaa tgactgccgt
     4861 tgagcatgcg caacttcatc tcttcataag gcagcacatc actaaccagt tcggctcccg
     4921 ctttttccca ttccggacgt ccggcaacaa agttatcttc tattacccac tggcggaaag
     4981 gttcacaggc aacgccagca ggatcgcgca caccggtaag ttgttcgatt ttcgccagcg
     5041 tatcctctgt cactgcgggc acaatacggt ccaccattgt tgatgggaaa gtcacgttta
     5101 cttcgatcca ttgtgccagt tttacatcaa cagcttgcgc gtaggaagtg acaacgtcac
     5161 gcataacatg accgttttct ggcatgttgt cacatgacat gacggtaaat gcgggaagtc
     5221 ctgccgcttt acggcgagcc agcgcctcaa caatcacccc tgttgcagtt ttcggctggt
     5281 ggggattttg cacgtcggca gcgaccatcg ggtgatcgag cattaactgt ccggtcgccg
     5341 gagagtggaa ataccctttt tcggtgattg tcagagagac aatcgcgatt tgcggttcac
     5401 acatcgcagc caacacggtt tctaagccat ctatctgtac gtgcaaggct tttttaacga
     5461 cgccaacgac gcgagccgtc cacgcatcgg ccgacatttc cgcaacggta taaagattat
     5521 cttgctgttg taaatcggca atttgctgtt cgccgccgat taaattgacc tcataatatc
     5581 cccaatcact gaaatgtttt gtagcaagaa tatcggcata cacgccctga tgcgcacggt
     5641 gaaatgcgcc aaagcctaaa tgaacaattc ttggggccaa gttattacga tcataaacag
     5701 ggagcgtcgc ttttgctgat aacaaattat ttcccataac aattccttaa atttaaatat
     5761 ggcaagctgc atgttttgtt atatgaataa aaaatcccct ctccggtaag agaagggatg
     5821 gaggatttac agacttcttg aaggttgcgc agctgttaca acacgcggtt gatcttccgc
     5881 ggcatcttcc agtgcactta aatcacggtc tctcacttct ggcattttca gcgcagagat
     5941 taaaccaatc actgaatatg ccatgatcat aatggcgatc ggataccagg attccgtcat
     6001 ggtgcagaaa atacccgcca ggataggacc aaaaccggaa gcgataagac caccaatttc
     6061 tttagaaatc gccatccggg taaagcggtt tttacagccg aacatttctg ccatggtaat
     6121 gttttccaga gcaaataatc ccagcaccgc acagttatga atcacaatca gtgcaaccat
     6181 aatggtgctc ggggcatagc ttttatctac gatgatagaa agcattggcc acgccagcac
     6241 aatcgcagag gtattcataa taatatacgg gatccggcga cctattttat cggataacca
     6301 accaaggaac ggaatggtca taaagccgag aatcgaactg atcatcaatg catctgttgg
     6361 aattgctttg ttaaacaata acgtctgcac taaatagcct gcaaggaaag tctgaattaa
     6421 accggagtta ccagcctgac caaaacgcag ccctgttgcc agccagaagg atttgctctg
     6481 gaacatgcta ccagcaggtg caggttttgc tgtcggttgg ttgctgtcgt taaccttctc
     6541 aaagaccggg ctttctttca gattcatacg caaccagata gcaaagacca tcacgacaac
     6601 gctcgccagg aacggtatac gccatcccca cgcaagcagt tcctctttac tgagaatgaa
     6661 gaacataaag gcccagattg ccgttgcgct caaggttccg cagttagtcc ccatagcaac
     6721 aaatgaggag ataattccgc gcttaccttt tggtgcatat tccgccagca tcgtgccggc
     6781 accggaaatt tccgcgcctg cccccagccc ctgaataata cgcagcgtca ccagcaaaat
     6841 tggcgcaaac acaccaattt gtgcataggt cggcagcaca ccaattaagg tagtacagat
     6901 ccccatcata gtgatggtaa taaagagcac ttttttacgc ccgattctgt cgcccatttt
     6961 gccgaaaata aatgctccga caatacgcgc cacataacct gcaccgtagg ttcccattgc
     7021 cagaattaac gccattgccg ttgatgattc aggaaaaaat atttcatgaa acactaacgc
     7081 tgcgccgagc gaatataact ggaaatccat aattcacagg tgttttttcc catcctgtgg
     7141 tttccttggc gttttctagg ttttttcaga tagttgcatt ttttaaaaag catcctaagt
     7201 tcgatctcag tgtctatctg gggcctattt ctgtcccata tatgccccaa aaaaaactcc
     7261 ccaacagata agtagttttt tcatggattt atgcgtaaaa tcaagaacgg ctggaaatca
     7321 ttcaatactc acactatcga aaaatttacc agccaaccgc agcacgttct tgcataaggc
     7381 gtgtctgcgg tttttcaact attcagatac atcactccca tcacattcat tcctccgcat
     7441 caaaggcata taggctatat caccttgata tttttctttt tcagataaaa actgttatct
     7501 atgtatactt ttaaacccaa tccgtgtaga gtctctgcat aagatagttt acagttgccg
     7561 cttcagcttg cgccataaac cgcctgattt ttgctgccac ctgttagcat tcctgtatac
     7621 ctgaaacgac aatgtttatc tacgaacttt aagaacaccc aagataaaaa ttgtcaacta
     7681 tatcatatat aacacattac taattcgagg ctatatgaac agcatactga taatcacatc
     7741 tctccttatc atattcagca tttttagtca tgccctaata aaattaggga ttggcatatc
     7801 caataaccca gacaaaaccg atgtataagt caacatatcc tgaatcagac atacaatatc
     7861 gcaatgaaaa tcaataatat tttaaggaat atcttcatga aatcaaaaga caccctaaag
     7921 tggttccctg cgcagcttcc tgaagtaaga attatcctag gggatgctgt agtggaagta
     7981 gcaaaacagg gaagacctat caataccaga acattgcttg attacattga aggaaacata
     8041 aagaaaaaat catggctgga taacaaagaa ttattacaaa
//
LOCUS       DAYPHN010000189         7971 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000189 DAYPHN010000000
VERSION     DAYPHN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.189"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1215..1931
                     /gene="nanC"
                     /locus_tag="ACG0BW_004217"
     CDS             1215..1931
                     /gene="nanC"
                     /locus_tag="ACG0BW_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFK7261108.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1951..3057
                     /gene="nanM"
                     /locus_tag="ACG0BW_004218"
     CDS             1951..3057
                     /gene="nanM"
                     /locus_tag="ACG0BW_004218"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFK7261109.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     DDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLITVKDNKVTVQN"
     gene            3122..4102
                     /gene="nanS"
                     /locus_tag="ACG0BW_004219"
     CDS             3122..4102
                     /gene="nanS"
                     /locus_tag="ACG0BW_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFK7261110.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGACWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            4212..4417
                     /locus_tag="ACG0BW_004220"
                     /pseudo
     CDS             4212..4417
                     /locus_tag="ACG0BW_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4685..5925)
                     /gene="yjhR"
                     /locus_tag="ACG0BW_004221"
                     /pseudo
     CDS             complement(4685..5925)
                     /gene="yjhR"
                     /locus_tag="ACG0BW_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_176259710.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase YjhR"
     gene            6041..6172
                     /locus_tag="ACG0BW_004222"
     CDS             6041..6172
                     /locus_tag="ACG0BW_004222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261111.1"
                     /translation="MTQVLIGDIYLRQQCDVFWLGYTISPAYARQGYAIEAITATID"
     gene            6663..6920
                     /gene="topAI"
                     /locus_tag="ACG0BW_004223"
     CDS             6663..6920
                     /gene="topAI"
                     /locus_tag="ACG0BW_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HFK7261112.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            6932..7477
                     /gene="yjhQ"
                     /locus_tag="ACG0BW_004224"
     CDS             6932..7477
                     /gene="yjhQ"
                     /locus_tag="ACG0BW_004224"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7261113.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCADPDETGALT"
     gene            7533..>7971
                     /locus_tag="ACG0BW_004225"
     CDS             7533..>7971
                     /locus_tag="ACG0BW_004225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HFK7261114.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQL"
BASE COUNT         2281 a         1822 c         1753 g         2115 t
ORIGIN      
        1 ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt
       61 aacaagtaaa atcaacataa aaaattcagc aaaatttaat attcgttcaa ctagccgtct
      121 cacatatgac attattagtc aactccattt gccacaaaat ggctatttca tggagcgcag
      181 taatttctta gtggctatta tcatgctaat tcttaggtcc ctagcgatta ttcctgctga
      241 ttcgtcagct tacgccatga gattgttaca ttccttttta tcctggagat atgggggaac
      301 gcaggagatg attttttcat ctacattcgt tggtaaatat agctttggtc actacgactt
      361 aaaccttcct caatcacagc cattcgccgg gcgatacggc aaacaaacta agtgaaacat
      421 catcctgaaa acaccaacat caacaagcct ctccagatcg acttcagaag tgaccagtta
      481 caagccacaa accaggaaac atatttcatg atgagaatta tgctcagcct gatggcgggt
      541 aaacactgat tttcaccaag ctcactgagt tagcttgcaa agctcccctt gtttagactc
      601 taaataaatt atttataaaa caaatagtta aactaagatc tttagttttt gaagaccacc
      661 gcaagtgttc gtctggcttc acatggcatc ttcctcttag aaaaagatcg acatattttg
      721 tgacacgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga tctggtataa
      781 caggtataaa ggtgcaccaa gatagtcaat gagacagggc atctcgcaat ctatggcaaa
      841 catcacttca gttctttctc atcgggtgat gaaaacgcac ttcagtctga aaggaatatg
      901 aaaatgagat caacagacat tctattttat gactctgggt aaaatggatt gagtaagtga
      961 tatagcttac gaacattcaa atcaattaaa catcagaaga gattttatac tcaggtattt
     1021 aatctggatc tctgtttatt taaataatgt gaaaagagat ttttcacagg agaccttata
     1081 caaaaaaata taaaatacag ctaccggttg ccaaagacac tataagcctg gcaaaaaaat
     1141 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
     1201 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
     1261 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
     1321 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
     1381 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
     1441 atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
     1501 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
     1561 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
     1621 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
     1681 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
     1741 aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc
     1801 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc
     1861 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
     1921 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc
     1981 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
     2041 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
     2101 gcatggtaca agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc
     2161 cctggcggac caagagatca agcaacctct gcatttattg atggcaatct gtatgtgttt
     2221 ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt acacaaatac
     2281 aaccccaaaa ccaatagttg ggttaaattg atgtcgcacg cgccgatggg catggcgggc
     2341 catgtgactt ttgtacacaa cggcaaggct tatgttactg gtggtgttaa ccagaatatc
     2401 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     2461 atcaatgctc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     2521 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     2581 gctggtgcgg cggttgtgaa taaagatgat aaaacctggc ttattaatgg cgaagccaaa
     2641 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     2701 aataagcttg ctcccgtctc atcaccagat ggcgtagctg gcggttttgc ggggataagc
     2761 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag
     2821 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     2881 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     2941 tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg
     3001 gtgacggatt cagttttgat cactgtgaag gataataaag tcacagtaca aaactaacgc
     3061 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac gcgaaattta
     3121 tatgaacgca ataatatcgc ccgattatta ctatgttctt accgttgctg gtcagtctaa
     3181 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag
     3241 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa
     3301 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     3361 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     3421 acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg
     3481 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     3541 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     3601 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgcatgct ggatgcaagg
     3661 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
     3721 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     3781 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga
     3841 agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     3901 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     3961 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     4021 tcatttcagc actgcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt
     4081 gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc
     4141 tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg
     4201 aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata
     4261 aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga
     4321 ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg
     4381 ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat
     4441 agacgatgat gatatgctta atgatgttca aagaaattag gccattatta tattattgct
     4501 tgcattttct ttcactccta aaagcacccg ccattaatcc caggcgcatt atgcaaagga
     4561 aaatgattag gtaaacaata agatttataa tcaggaaagg cgactcgcaa taaaacggaa
     4621 agtcgcctta acattggtaa aatatgccct ttactactcc tgtatcatga acggctcatc
     4681 acatttaaag ctgacgttga tccagactac catatattgc ctttatttca ttttttacgc
     4741 cttcgccacg gtacaccaaa gacgtatcgt atcgttgata cttatcttcc cgtgtagcac
     4801 tgaaccagtt aaaagatccg acacacagca gttcttcatc ctcaatcaca attttgctgt
     4861 gaacacgatt aaccaatttc gtagcgatac ccatcttatt gagcttttca acagcatcat
     4921 tcagcagatg ttgtttctcc tgacgcttat catcatcgac atgcgctata ttgcagtttt
     4981 tatccgtcac cacggtaata tcaatgcccc gggaacgggc caaagccatt gatgccagaa
     5041 atcccgtttg ctccactttt tgccaggata accaagggga aataatcgtt attttctttt
     5101 gagctccggc aagagtcttg ttcaaaaatt catcatgctg ctccacacca tgcagcgttg
     5161 aaatttgtgt atgtgcggaa attaaatcct gtcgtttctg aaactcaaat tgcaacgcat
     5221 tattatctga agaaaagaga tattttgcca ataaccctcg cggcgaaaat gcgggctgca
     5281 tctcgataag gtccatatcg ccaaatacca gaaaactatc tttagcgcgt gagacagcaa
     5341 cattgaggat agtgctgttg ctatcaagaa acctgccatc ttcatgtttt gagtagacag
     5401 gagaaaagag aacaatcgcc ctttccgccc cctgcaagga gtgcacagtc cccactgtca
     5461 gtaaaccctg ctcgtctttg ccattaattt ccagcttacg caatgacatt ttgatagcat
     5521 taacctgcgc cgaaaatggc gtcacaacac caacaacttt gtacagtggt tccccgtaat
     5581 ggcgctctat atcatcctta tgtgccacca gccatgctgc tattgtttca gcttcaagag
     5641 ggttatgacg gctccctcca tttggcttca tgcctctacc atcaatatgt aaatatccca
     5701 ttgcggggaa aatcgtcctt tttccatccc tcttttaggc tgcaacttac cgtgatagca
     5761 aagtgagtta cagtagtcga tgatgttatt gaaacagcga cgatgctcat acagatacat
     5821 tccgcgctcg aacttgggat catactggta gtgagagttg aactgggcta cctgcattac
     5881 gctgccagat gctgcacttt ttccagaccc atttattatc gaatacggtg accgcctgag
     5941 cggccacctt taatcgtggg cagttacaca gaattattta cagggtcttg ctgggagttc
     6001 ttacacttgc tcgcgatatt caattggcca ttgtcaacaa atgacgcagg ttttgattgg
     6061 tgatatctat ttacgtcagc aatgcgatgt gttttggctc ggctatacca tcagtcccgc
     6121 ttatgctcgc cagggatatg caattgaagc aattaccgcc acgattgact gaataaaaga
     6181 gaacggtttt tcactcatca aagccggtgt gaatccggaa aatacccttt caaaaaaatt
     6241 acttatcagg attggcttca atttttcaag catcgaagat gggtgagcat atttatgttc
     6301 tggatttaac aaaaggagaa taatctcccg aagggaaaag ccgatgaggc catttcaggc
     6361 gtacttcaac atgcattcgt cagtgtcatc tgccttttcc agtaaatggg ttggcattca
     6421 atttccaaag tacatcacac tttttctact gaaacatatc cctcctataa ctacgtgtta
     6481 atattcgctc cgtaaatacc tttcaactca acccgaggct ttgatgctgg agaatgtaat
     6541 catccgataa tcagttcccc gaccttttca ggccggactg attatcaatg cgccgaaatc
     6601 gaatgcggac accgcggtgt gtttgcacgt tttgcacatg atgattaaat aggttcttca
     6661 gtatgaattt atcccgtcag gaacaacata ccttacacgt tctcgctaaa ggtagacgta
     6721 ttgcgcacgt ccgcgattct tcaggccgcg tcacttccgt tgaatgctac agccgcgaag
     6781 ggctgttgct gaccgactgc acgctcgccg tcttcaaaaa actcaaaacc aaaaaactta
     6841 tcaagtccgt caatggccag ccctatcgaa tcaacaccac cgagctgaat aaagttcgcg
     6901 ctcagctcga taatcgctaa ggagagtttc tatgacagta caccacttca cttttcatat
     6961 cacagataaa agtgatgcca gtgatatccg ggaagtcgaa acccgcgcat ttggcttcag
     7021 caaagaagcc gatttagtgg cctctttact tgaggatgaa agcgcgcgcc ccgcgttatc
     7081 gctgctagcc cggtatgaag gcaaagccgt cgggcatatt ttatttaccc gggcaacctt
     7141 caaaggagaa atggattcac cgttgatgca catcctcgct cccctggcgg tcataccgga
     7201 ataccaaggg atgggcgtgg gaggacggtt gatacgcact ggcatcgagc acctgaggtt
     7261 gatgggctgc cagacggttt ttgttctcgg ccacgccacc tattaccccc gacatgggtt
     7321 cgaaccctgt gccggagaca aaggctatcc ggcaccttat cccatcccgg aggagcacaa
     7381 agcctgctgg atgatgcaat cactcaccgc tcagcccatg accctgaccg gccacatccg
     7441 gtgcgcagac cctgatgaaa ccggagcact gacgtgaata acaatcttgt tgtgtccttt
     7501 ttaaggctta acccaataac aggaattaaa taatggatat cccacgtatt tttaccatca
     7561 gcgaaagtga acaccgcatc cataacccgt tcaccgaaga gaagtacgcc acgctgggtc
     7621 gcgtgctgcg catgaagccg ggcacccgca ttcttgacct cggcagcggc tcgggcgaga
     7681 tgctctgcac ctgggccagg gatcatggca ttacggggac tggcatcgac atgagttcgc
     7741 tcttcaccgc gcaggcaaag cgtcgcgcgg aagaactcgg cgtcagcgaa cgcgtacatt
     7801 ttattcataa cgatgcggcc ggttacgtcg caaatgaaaa atgtgacgtg gcggcctgcg
     7861 tcggcgcgac atggattgcc ggtgggtttg ccggggcgga ggagctgcta gcgcaaagcc
     7921 tgaaaccggg cgggatcatg cttatcggcg aaccctactg gcgtcagcta c
//
LOCUS       DAYPHN010000190         7882 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000190 DAYPHN010000000
VERSION     DAYPHN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.190"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1198
                     /locus_tag="ACG0BW_004226"
     CDS             <1..1198
                     /locus_tag="ACG0BW_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFK7261115.1"
                     /translation="ANENKLKVYNVHFDNNQELVADGEPDVVFLKKTVWEDLLVKLKL
                     ENKENAVSEINLSSNKNNVDQFIECAKRNEQTLFGNIRKSDFHVASLQPGRTRSVISE
                     TPPNDCMESHNLYENHTDKVSTVTKNSQQVKGHYGDKLKEMQLFLNQMSNALQQDSSL
                     LESKEHTIDIQEKTNKFVQHFQRVLFDKNGRSSEFLLNFYECCYKFLPRAQPQDKIDS
                     YNSALQAFSIFCSSTLTHNNVGFNFKLFPEVKLSGGELETVFKYKNGNFVWEIARIKI
                     ALPKEEGSLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTPCIFNVPDDNKS
                     YDKLLKSVSENGLNGVLTDRNNKIKLITGVAPFDGISSMDDDFDDSSSEENSPEDSPI
                     ENRPLV"
     gene            complement(1452..1613)
                     /locus_tag="ACG0BW_004227"
     CDS             complement(1452..1613)
                     /locus_tag="ACG0BW_004227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261116.1"
                     /translation="MSPTDNVLSRLFSPALLPRFYVEVVTLSTQFISQAWCTITLLLL
                     GKIKKLSGS"
     gene            1567..1716
                     /locus_tag="ACG0BW_004228"
                     /pseudo
     CDS             1567..1716
                     /locus_tag="ACG0BW_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1948..2067
                     /locus_tag="ACG0BW_004229"
     CDS             1948..2067
                     /locus_tag="ACG0BW_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFK7261117.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            2132..6706
                     /gene="ypjA"
                     /locus_tag="ACG0BW_004230"
     CDS             2132..6706
                     /gene="ypjA"
                     /locus_tag="ACG0BW_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFK7261118.1"
                     /translation="MNRTSPYYFRRSVLSLLISALIYAQPGMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVYKGGTSNDTTIKGGTSRIEGGTANGTIIDGGGQ
                     SVSTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTAIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSSSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVAENVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSEKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMVNGTAEGSIINGGSQVVNEGGLAENSVLNEGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQDAANNILLANGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAVKTTINEGGTL
                     TVNDNGKATDIIQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQALARAEDLLIAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGVVRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVADSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPAPTPTPVPENRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            7220..7549
                     /locus_tag="ACG0BW_004231"
     CDS             7220..7549
                     /locus_tag="ACG0BW_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261119.1"
                     /translation="MPETTPKPIAEYQGDTYCCDLKAVSEAIRLSGRPMMPGQYLFHG
                     GKWPAEYKYGAMFETTRPLSTTLCPNIAMGVPQWHDRAYRNNAIELFVLRVSEPHSKV
                     FSLTVKI"
BASE COUNT         2322 a         1588 c         1965 g         2007 t
ORIGIN      
        1 tgctaatgaa aataaattaa aagtatacaa cgtacacttt gataataatc aagagttagt
       61 tgctgatggt gagcctgacg tagtattttt aaaaaaaacg gtgtgggaag accttctcgt
      121 taaactaaag ctggagaaca aagaaaatgc ggtttctgaa attaacctgt catctaataa
      181 aaataatgtt gatcagttta ttgaatgcgc taagagaaat gaacagaccc tattcggcaa
      241 tataagaaaa agtgattttc atgttgcttc acttcagcca ggtagaacgc gtagtgttat
      301 ttcagaaacg ccgccaaatg actgtatgga atcacataat ttatatgaaa accacacaga
      361 taaggtttca actgtaacta aaaattctca gcaagttaaa ggtcactatg gagataagtt
      421 gaaagaaatg cagttgttcc tcaaccagat gagcaatgca cttcaacagg attcatcttt
      481 gttagagtca aaggaacaca ccatagatat tcaagaaaaa acgaataagt ttgtgcagca
      541 ttttcagcgg gtattatttg ataaaaatgg aaggtcatca gagtttctac ttaattttta
      601 tgagtgttgc tataagtttt taccaagagc gcagcctcaa gataaaatcg atagctataa
      661 ttcagcactg caagcttttt ccatcttttg ttcatctacg ttgacacata ataatgtagg
      721 gtttaatttc aaattatttc cagaagtcaa gttgtctgga ggagagcttg aaacggtatt
      781 caaatacaaa aatggcaatt ttgtctggga gatagccaga attaaaattg ctctcccaaa
      841 agaagagggt agtttatata atttacgtgg attggatttt aagggatgct tcttttctgg
      901 acagaacttc agtaactatg atattcaata tgtgaactgg ggaacgtcat tgtttgatgt
      961 tgatactccg tgtattttta atgtgcctga tgacaataag agttatgata aattattaaa
     1021 atccgtcagc gaaaatggtt taaatggagt cttgactgat cgtaataata aaataaaact
     1081 aatcacgggc gtggcaccat tcgatggtat ttcatctatg gatgatgact ttgatgatag
     1141 ttcctctgag gaaaattccc ctgaggatag tcccattgag aatcgtcccc ttgtataaag
     1201 ttctaaaagc agttttcaat gataaataaa ctgcaggttt tacaaagaga taataacgta
     1261 gattgaaaca atatgtatta ttgattgatt aaacgggatt attttttaca ctacaaccat
     1321 tttgctgcta ctaccaaaga aaaaggggct acgctttcac gtaacccctt gatctgcgct
     1381 gtgatttact gagtttaaat tggtgctgta attggttgaa atatttcatc taatgtctga
     1441 ttcaactttc cttagctgcc tgaaagtttt ttgatcttac ccagcaacaa cagcgttatg
     1501 gtgcaccatg cctgactgat gaactgcgtg ctcagggtta ccacttcaac gtaaaaccgt
     1561 ggcagcaggg ccggactgaa caaacggctc aggacattat cggtaggtga cacactggtt
     1621 gtctgcaaac tgaacctact ggggcgcagt atgcggcatc ttgccgtgct gttggaggag
     1681 ctgtgcgaac gtggtattaa ctttcgtgct ctgactctac caatatttgc ccaacaatga
     1741 ggggacgaaa gctgtaaaag taaaacaatc tgcaatctca atgttattgt gtgattttta
     1801 tgtgagcaga agatattcat cagaaacgat tatgtaaatc attttatttt tccgatggct
     1861 tcaatgtcga aagaaaagaa tacgccgtat ttcctgaaaa gattgacttt tttatccaat
     1921 catccttcag cacaatgtct taaaaaaatg cctcattctt atgcggaata tcatcatttc
     1981 atcatgatgt ctttgatgag cggtgaacac aatacacttg cgctgtctct tcaggatgaa
     2041 tcccctggtc tggtgcctgt gggctgatgt tgcagcaaag ctaaggtcgc ttaaacgcta
     2101 ctcagtattc acttttcaga ggatgaaatt tatgaacagg accagtccct attattttcg
     2161 ccgctcagta ctttccttat tgatatctgc cttaatatat gcccagcccg ggatggcggc
     2221 cttcactact aatgttattg gtgtggtaaa cgatgagact gtagatggca accaacgagt
     2281 agatgaacga ggtacaacaa ataacacaca tattatcaac catggccggc aggaggtcta
     2341 tggtgggata tctaatagta gtattattga aactggtggc gagcaactag tatcaatcca
     2401 tgccgatatt aacgggcaag ctaataatac aaccattaat ggaggcagac agtcaattga
     2461 gtacggagga atttctactg gcacgataat cgaaagtggc aatcaatatg tttataaagg
     2521 tggtaccagc aatgacacga caattaaggg cggtacttca cgcatagagg gggggactgc
     2581 aaatggaaca atcattgatg gtggaggcca gtcagtatca actcaggggc atgttgacgg
     2641 tacgacgata aataagtctg gttatcagga catcacgcaa ggaagtctgg cgacgaacac
     2701 aaccataaat ggtgggcgac agtatgttga acagagcaca gtagaaacaa ccgccattaa
     2761 aaatggcggt gagcaaagag tatatgagag ccgtgcgctg gacacgacga ttgaaggcgg
     2821 aactcagtct ctgaatagta agtcaacggc aaaaaatacg cagatctatt ctggtggtac
     2881 gcaaattgtt gataacacga gctcctcgga tgttattgaa gtttattccg gcggcgtgct
     2941 tgatgttaga ggtggtacgg caactaatat tactcagcac gatggagccg ctttaaaagt
     3001 aacgacttac gatttgacgg tgagcggtac gaatagtgaa ggggcattct ccatccacaa
     3061 taacgtggca gagaatgtgt tgctggaaaa cggtggtcat ttagacataa acgcatatgg
     3121 ttcggcaaat aagacgatta ttaaagataa aggaacaatg tcagttttaa cgaatgctaa
     3181 agctgatgcg acccgaatag ataatggcgg cgttatggat gttgccggaa acgcgacaaa
     3241 taccataatt aatggtggca cacagaatat taataatcat ggtattgcca cgggcaccaa
     3301 tatcaacagt ggaacgcaaa atatcaagag cggcgggaaa gctgacacga caaatatatc
     3361 ctctgggagc cggcaggttg ttgagaaaga tggtacggca actggcagca atattagcgc
     3421 cggaggctcg ctgattgtct ataccggcgg tattgcacat ggggttaacc aggagacggg
     3481 cagtgcttta gttgccaaca cgggcgcagg gactgatatc gaaggataca acaagctctc
     3541 tcacttcact attaccggag gagaggctaa ttatgttgtg ctggaaaata ccggcgaact
     3601 gacggtaatg gctaaaacct cggcgaaaaa tactaccatt gatgctggcg gtaagctgat
     3661 tgtccagaag gaggctaaaa cagatagcac cagacttaat aatggtggcg ttctggaggt
     3721 tcaggacggt ggtgaggcta agcatgttga gcaacaatcc ggcggcgcat taattgcttc
     3781 cacgacctcc ggaacactta tcgaaggaac caacagttat ggtgatgctt tctacatcag
     3841 gaattcagaa gctaaaaatg tagtgctgga aaacgctggc tcattaacag tcgtcactgg
     3901 ttcccgggca gttgacacga ttattaatgc caacggcaaa atggatgttt atggaaaaga
     3961 tgttggtact gtacttaata gtgctggtac ccaaacaata tatgccagtg ccacttctga
     4021 aaaagcaaat atcaaaggtg gcaagcaaac ggtatatggt ttagccactg aggcaaatat
     4081 cgaaagtggt gaacaaattg ttgatggtgg gtcaacagac aaaacgcaca tcaatggcgg
     4141 tacgcaaacc gttcagaatt atggtaaggc gatcaatacc gatatcgtct caggcctaca
     4201 acaaattatg gtaaacggga cagcggaagg ttccattatt aatggcggtt cacaggtagt
     4261 taatgagggc ggtctggctg aaaactcggt gcttaatgaa ggcggcactc tcgatgtgcg
     4321 ggagaaaggc agcgcaacgg ggatacagca aagtagccag ggcgcgttgg ttgcaaccac
     4381 cagagcgacg cgggtcacag ggacacgcgc tgatggcgtc gcgttcagca ttgagcagga
     4441 tgcggcgaac aatatcctgc tggcaaatgg cggcgtgtta accgtggagt cagacaccac
     4501 ttccgccaaa acacaggtca atgcgggcgg gcgggagatc gtcaaaacaa aagccactgc
     4561 gacaggcacg acgctcaccg gcggcgaaca aatcgtcgag ggggtggcga atgagacaac
     4621 gattaacgac ggcggaatac aaacagtttc ggctaacggt gaggcagtaa aaacaacgat
     4681 taatgaaggc ggtacgctga cagtcaacga taatggcaaa gcgacagata tcatccagaa
     4741 cagcggtgcc gctctccaga cgagcacggc taacggtatt gaaatcagcg gtactcacca
     4801 gtacggcacc ttttccattg ccggaaattt agcgaccaat gtgctgctgg aaaatggcgg
     4861 taatttattg gtattagcag gtaccgaagc tcgcgattcc acggttggca acggtggggc
     4921 aatgcaaaac ctgggtcagg acttcgccac aaaggttaat tccggtgggc aatataccct
     4981 cggtcggtca aaagatgagt ttcaggctct ggcccgggca gaagatctac tgatcgctgg
     5041 cgggacggca atcgtctacg caggtacgct ggcggatgca tcggtcagtg gcgcgacagg
     5101 aagcctgtcg ttaatgacgc cacgggataa tgttacgcca gttaaacttg aaggggtggt
     5161 ccggattacc gatagcgcga cattaactat cggaaatggc gtcgatacga cgcttgccga
     5221 cctgacggct gccagtcggg gcagtgtctg gcttaacagc aataattcct gtgcaggcac
     5281 cagcaactgc gaatatagag taaacagttt actcctcaac gacggtgatg tttatttatc
     5341 agcacaaaca gcagcgcctg ccacaactaa cggtatctac aatacgctga ccaccagtga
     5401 actttccggc agcggtaatt tctacctgca taccaacgtt gcagactccc ggggcgatca
     5461 actggtcgtc aacaacaacg ccactggcaa ttttaaaatc tttgttcagg ataccggcgt
     5521 cagccctcag tctgacgacg cgatgacgct ggtgaaaaca gggggagggg atgcttcgtt
     5581 tacgctgggt aataccggcg gtttcgttga tcttgggacc tatgagtatg tcctgaaaag
     5641 cgacggcaac agcaactgga acctgaccaa tgatgtcaaa cccaacccgg accccaaccc
     5701 aaatccgaaa ccggatccaa aaccagaccc aaaaccggat ccgaaaccag acccgactcc
     5761 cgctccaacg ccgacacccg tcccggagaa tcgcatcacg ccttctacag cagccgtact
     5821 caatatggca gcaacattac cgttggtctt tgatgctgag ctaaacagta ttcgcgagcg
     5881 gttgaacata atgaaagcga gtccacacaa caataatgtc tgggggacga cgtataacac
     5941 ccgtaataat gtcaccaccg atgcgggtgc cgggtttgag cagacgctga ccggaatgac
     6001 ggtggggatc gatagccgta atgatattcc tgaggggatt gcgacgctgg gcgcttttat
     6061 gggttattcc cattcacata ttggttttga tcgtggagga catggcagtg tgggcagtta
     6121 ttctctgggg ggctatgcca gctgggaaca tgaaagtggt ttctatctgg acggtatcgt
     6181 gaagctgaac cgctttgaaa gtaacgtagc cggtaaaatg agcagcggtg gagccgccaa
     6241 cggcagttat cgcagcaacg ggctgggcgg tcacattgaa accgggatgc gatttacgga
     6301 tggtaactgg aacctgacgc cgtatgcatc gttaacgggg ttcaccgctg ataaccccga
     6361 atatcattta tccaatggca tggaatcgaa atcagtcgat acccgcagta tatatcgtga
     6421 actgggcgca acgctgagtt acaacatgcg tctggggaac ggtatggaaa ttgagccgtg
     6481 gctgaaggcg gctgtgcgca aagaatttgt cgatgataac cgggtgaagg tgaataatga
     6541 cggtaatttc gtcaatgatt tgtcgggcag acgtggaata taccaggcag gtattaaagc
     6601 ctcattcagc agtacgttaa gcggacatct cggggtgggg tatagccatg gtgccggtgt
     6661 ggaatccccg tggaacgcgg tggctggtgt gaactggtcg ttctgaccat caacgaaaaa
     6721 gcccacatct gtgggctttc atatcaccag gagccgcggc tcctttgcgt atccttttat
     6781 gtctcctcac cgtctggtcg gtgtcttgct gagactgcta acttcctgtt tttgttggtg
     6841 ttgtccttgc accgtccaat catgattggc tggcgtcctt gtaattggtg gggtaggtga
     6901 ttgggtttgt tgaggatttt gattttttgc gcgaagggga aggcgcaaaa agagtggtga
     6961 tgaagttgat atggaaggga ttttaatatc cgattgattt ttaagttgat tgttgggggc
     7021 tgggttttgc gtgttggttg aggctttggt ggacgttata tgcttgtcgt tcgtttccag
     7081 aagtgagcgt ttctgtcgaa taattcactc tctttaaatt tccgggcgga aaaacatgct
     7141 tatctaatac acactaacaa ccgaaggcga taccggtatc gaagacgcta taaagcaaca
     7201 cccttcgtgg ttgtcttaca tgccagaaac tacacctaag ccgattgcag aatatcaagg
     7261 agatacttat tgctgtgatc taaaggcagt atcagaagca attcgcttaa gtggtaggcc
     7321 gatgatgcca ggtcaatatc tatttcatgg cggaaagtgg cccgctgaat ataaatacgg
     7381 cgctatgttt gaaaccactc ggccattgtc aacaacatta tgtcccaata tcgcgatggg
     7441 agttccacag tggcatgata gggcatatcg taataatgca attgaactat ttgtacttcg
     7501 ggttagtgaa ccacattcaa aagttttttc tttaacagtg aagatataga tacaggacac
     7561 gagaaataac tggtttttga gcaaggggta ataataacac tgacaagctt cgaggatatt
     7621 caaaaaattt ctgttgccga atttgctctt agtcgctcag tacaaaacac caaagacgta
     7681 cctgtacgag ttttagctat cgaaatatcc tgatttcaaa atattggtca ggggatgggg
     7741 ctacgaaata cagctcggct ttttttcatt gccccgttct cttcacaatg cttaacagtt
     7801 ttgtttacaa ctgtcataaa aattaaaaat gcaaatataa taattgtatc tttaataaag
     7861 tataattaaa tgcccgaagg at
//
LOCUS       DAYPHN010000191         7860 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000191 DAYPHN010000000
VERSION     DAYPHN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.191"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            32..682
                     /gene="zinT"
                     /locus_tag="ACG0BW_004232"
     CDS             32..682
                     /gene="zinT"
                     /locus_tag="ACG0BW_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFK7261120.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDTQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1352..1441)
                     /locus_tag="ACG0BW_004233"
     tRNA            complement(1352..1441)
                     /locus_tag="ACG0BW_004233"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1405..1407),aa:Ser,seq:cga)
     gene            1535..2332
                     /gene="mtfA"
                     /locus_tag="ACG0BW_004234"
     CDS             1535..2332
                     /gene="mtfA"
                     /locus_tag="ACG0BW_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFK7261121.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2433..2508
                     /locus_tag="ACG0BW_004235"
     tRNA            2433..2508
                     /locus_tag="ACG0BW_004235"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2466..2468,aa:Asn,seq:gtt)
     gene            2670..3932
                     /locus_tag="ACG0BW_004236"
     CDS             2670..3932
                     /locus_tag="ACG0BW_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7261122.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLQKGIEEKGLLEV
                     ASRTRQHLSNIIRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVHYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKTMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4126..5430)
                     /gene="ybtS"
                     /locus_tag="ACG0BW_004237"
     CDS             complement(4126..5430)
                     /gene="ybtS"
                     /locus_tag="ACG0BW_004237"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFK7261123.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5458..6738)
                     /gene="ybtX"
                     /locus_tag="ACG0BW_004238"
     CDS             complement(5458..6738)
                     /gene="ybtX"
                     /locus_tag="ACG0BW_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFK7261124.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIVSDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQACLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6731..>7860)
                     /gene="ybtQ"
                     /locus_tag="ACG0BW_004239"
     CDS             complement(6731..>7860)
                     /gene="ybtQ"
                     /locus_tag="ACG0BW_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFK7261125.1"
                     /translation="DADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVV
                     VLSGIVWVVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAI
                     APLPVAEQSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKT
                     TVTKLLMRYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIAR
                     PQATRQEVEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAP
                     VVILDEPTAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVE
                     QGTHAQLLSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
BASE COUNT         2014 a         2121 c         1895 g         1830 t
ORIGIN      
        1 atcatgtaca ttaactctgg aggaaattgt tttggcgatt cgtcttcata aactggctgt
       61 tgctttaggt gtctttattg ttagcgctcc tgccttttcg catggtcatc attctcatgg
      121 caaaccctta acagaggtcg aacaaaaagc tgctaatggt gtttttgatg ataccaatgt
      181 acaaaaccga acgctcagtg actgggatgg agtctggcaa tccgtttacc ctttactgca
      241 aagtggcaaa cttgatcccg tctttcagaa gaaagcggat gcagataaaa ccaaaacatt
      301 tgctgaaatt aaagattatt atcgcaaagg ttatgcgaca gatatcgaaa tgattggcat
      361 tgaggacggc attgtcgaat tccatagaaa taatgaaaca acatcctgta aatatgatta
      421 cgatggatac aaaatactca cctataaatc aggcaagaaa ggcgttcgtt acttattcga
      481 atgtaaagat cctgaaagca aagcccctaa atatatacaa tttagcgatc atataattgc
      541 accacgaaaa tcatctcatt ttcacatttt tatgggtaat gatacccaac agtctttatt
      601 gaatgaaatg gaaaactggc caacgtatta tccctatcag ttgagtagcg aagaagtggt
      661 cgaggagatg atgtctcatt gatttttttg cgctgacttc agttatttaa cagaacagtg
      721 cctggtagta acaacaataa tttaccaaga caaataagta agacaaactc agacacgctc
      781 atacttgagt cgtgtctttt ttattatgtc tgtagaaaaa aactgcgaaa aaggcgaaca
      841 ataaatacac aactcagcca tacagactag cagcgcaaat gtttccagga gcaagtcatc
      901 tgtcatggta aagctaacca taaatatagg ttaattttga tgtttaaacc ttttttactt
      961 gttagtatga tacaaaggct ttcaaaaaag ctgggaaatt caacaactct tggaaattat
     1021 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttggttc aattattgac
     1081 cccttctcta cgcaacttca gttcccgcca gcaactttgc ggcagtttca taggatcaat
     1141 gtctaaaaga ataatggtga ccgataagaa aacgactgaa taactgtaga ttttcgctcg
     1201 aaaccttcct gtcagaccca tagcgaatca agtgctgaat gtcacagtat cgaacagaaa
     1261 acagtgacga tctaaccctt caagaatatt ctacgattgt tctgtttagg aaaagcaagg
     1321 cgggaagtcg ggagataagt cattgataaa gtggcggaga gagggggatt tgaacccccg
     1381 gtagagttgc ccctactccg gttttcgaga ccggtccgtt cagccgctcc ggcatctctc
     1441 cgttcagatg gttgccatga tgccaggaaa tttggcattt taacagtccc tgtccgtgca
     1501 attttgttca agtgacgagt ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca
     1561 agaatcagca catcaaactg cccttccctg gcaggaagca ctatcgatcc cccttttaac
     1621 gtgtctgaca gaacaggaac aaagcaaatt ggtcgctctt gccgaacgtt ttttacagca
     1681 aaaacggctt gttcctttac agggctttga gctgaattca ttaagaagct gccggatagc
     1741 acttctattt tgcctgcccg ttctggagtt aggactggaa tggctggatg gttttcatga
     1801 agtcttaatt tatcctgcgc catttgtggt cgatgatgaa tgggaagacg atatcggtct
     1861 ggtgcataac caacgtattg ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt
     1921 gaactggttg gatatacaag attcttttga tgcttctggt tttaacctga ttattcatga
     1981 agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc agcggagttc cctttatttc
     2041 gttgcgtgag gttgctggct gggaacacga tcttcatgct gcaatgaaca acattcagga
     2101 agaaatcgaa ttagttggtg agaatgcggc gagcattgat gcttatgctg ccagtgatcc
     2161 tgctgaatgt tttgccgtac tttctgaata tttctttagc gccccagaac tttttgctcc
     2221 tcgtttccct tcattgtggc aacgtttctg tcaattttat caacaagatc ctttgcagag
     2281 actgcatcac gctaatgata cagactcgtt ttcggcgacg aatgttcatt aattaacaac
     2341 tttgcagatt aattaaccaa ttgaaatggc ttatgaaatt tagtgttgac agacaaggta
     2401 ccgctaagta atatgcgccc cgttctcacg attcctctgt agttcagtcg gtagaacggc
     2461 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaat tttctgtttt
     2521 cttgcatcct tgcgaatctt tatctgatgt tgattcaaca ggttagtgtg aaaactcttc
     2581 tcggtgcatt ttgattttac cctacgcatc gggaaaaatt ggtggtcaaa tctggggtca
     2641 ggttagttcg ataatggagt gacccccata tatcccttac cgacgcaaaa atccgcaccc
     2701 tcaagccttc tgataaaccc tttaaagtct ccgattctca cggtctgtat ctgcgggtca
     2761 agccgggtgg ctcccgccac tggtatctca aataccgtat tagcggtaaa gaatcccgca
     2821 ttgcgctggg tgcctatcca gccatctccc tgtctgatgc gcgacagcaa cgtgaaggta
     2881 tccgtaaaat gctggcgctg aatatcaacc cggtacagca gcgggctgct gtacgtggct
     2941 cacgaacacc ggagaaagtt tttaaaaacg tggcgctggc gtggcataaa agtaacagga
     3001 aatggtcgca gaacaccgcc gaccgtctgc ttgccagcct gaacaatcac atctttccgg
     3061 tcatcgggaa cctacctgta tcagaactta aaccccgtca tttcattgac ctgcagaaag
     3121 ggatcgagga aaaaggtctg ctggaggttg cgtcccgcac acggcagcac ctgagtaaca
     3181 taatacgcca tgcggtccat caggagttaa tcgatacgaa ccctgcagca aaccttggcg
     3241 gcgtgaccac acctcctgtc agacggcact atcctgccct gccgctggag cggctgcctg
     3301 aactgcttga acgtattggg gcatatcatc agggccgtga actgacccgg catgccgttc
     3361 tgctgatgct gcatgtgttc attcgctcca gtgaactgcg tttcgcccgc tggtcagaga
     3421 ttgatttcac aaaccgagtc tggacgatac ccgcgacgcg agaacccatt attggcgtgc
     3481 attattccgg ccgcggggca aaaatgcgaa tgccgcatat cgtccccctc tcagaacagt
     3541 ccatcgccat tctgaaacag attaaggata tcaccggtaa taatgaactg atcttccccg
     3601 gcgaccataa cccgtataag acaatgtgtg aaaacacggt caataaggca ctgcgggtga
     3661 tgggttacga cacgaaaaag gatatctgcg gtcacggctt ccgggcaatg gcatgcagtg
     3721 cgctgatgga atcgggttta tgggcaaagg acgcagtaga acgccagatg agtcatcagg
     3781 agcgcaatac cgtgcgcatg gcttatattc ataaggcaga gcacctggaa gcccgcaaag
     3841 cgatgatgca gtggtggtcg gattatctgg aagcatgccg agaatcttat gcaccgcctt
     3901 atacaattgg taaaaataag tttatcccat agtatactaa cgaatcccac actctggcaa
     3961 attaccacca cctccacagg aggtggttct atatagactt ttatcaactt catgacgaaa
     4021 aacacctatt ttatcccaaa agaagacaat agagtcacag aggaatcaaa gagcgactca
     4081 attgtctttg gacagtctgg ttgtgaggtt agaaaacagt tactcctaca ccattaaata
     4141 gggcgcaatg ctcgctaatt tctcccgggt ttctgtcagt tcgcgttccg gcgtagattg
     4201 cgcgatgatt cccgcccccg cctgtatcca gcagcgctgg ctatcctgaa atacggaacg
     4261 cagaactagc gccgcatcaa agcgcgtatc gtccagcagc aggattgcgc cggaataaag
     4321 ctctcgcggc gtcttctcaa tttgcataat cgcgttcaga gcagcatttt tagggatacc
     4381 tgaggcggta atcgacggaa acagcacggt aaacgcatcc caggcatcct tgttttccgc
     4441 aagctgaccg ctcacgccag accccagatg ctgaacgctg ccgcgctggc gaaccgacat
     4501 taaatcttca accaccacgc tgcccggctg gcaaacagcc tccagttcag caatagcttc
     4561 tttgacagaa aggatatgct caagcacctc tttactgtcg tgcagcagtt ctgcctcttt
     4621 cgccttatta tgctccgggt ttcccatgcg atcgcgggtg cccgcaagcg gttcagtgac
     4681 cactttattg cccgtcactg acatcaccag ttccgggcta aagcccagcg cttcgcgtcc
     4741 ttcctgacgg aacataaacg agcgcacagg cgtgtttgcc tgccgcccgt ataacagcgt
     4801 ggcgggcata tcaatccgcg atggcagggg aatggcgcgc gagacaatca ctttgacata
     4861 ctcgccacgg cggatctccg caacggcgcg cgcaacctgt tgtttatatg cctcaccatt
     4921 gagggcggta tccaccgcca gtggtgcgtt ctgcgtcgtt gtaccggcct cttttaccca
     4981 ctcgcacaaa cgtcggcacc cgtcggcgga gtccgcataa acggtgacat ttcccttttc
     5041 aaaaataagt tcttcacggg gaacggttaa cgtcagcagc ggccactccc cggcgttaaa
     5101 ggcaatcccc cgcgcatgcg ccgcaaagtt gaacccaacc tgtccatata cccgacgtcc
     5161 tttcacctga ggatgcgcca taaaacgacg cgcgcagtcg gcaatggaat ccaccgccca
     5221 tttttgctcc ccggcatcat caataaatgt cgcctgcgtt ccgtcggcat taatgtgcag
     5281 ccgtgcctgg caccctttgc ctaaatacca acagggttga cgctcataga cataacactc
     5341 ttcttctgcg aactggcgta aaacgccaga aatcgtcggt agccattgtt cctctggtaa
     5401 tgccagatgt agaaattcac tgattttcat cccttacctc tctgtgttat tcccggatca
     5461 atcaattgcg cttgtcatca gttctcttgc cgaatgcagc cggatataag caacccacgc
     5521 cgccagcaac gtgaatgccc cggcaaacag gaagcaggcc gcatagccaa aatgttgagc
     5581 aacaacgcca ccgataacgc ctgccagaat ggcgattgcc gcgtcagtac attgaaagag
     5641 ggtgaagtcg acaccggcct gcaaaggcga tgaaagcgac atcagcgtgg cgtaaagaca
     5701 gaccagcgcg caggccagcg aaatggactg gacaatgacc agacactgga gaatctgcag
     5761 cagcagatga cccggcgcca tcatgagcgt catcaccacc gccagcagcg cgatcccctg
     5821 gacgccataa gccgtcaaca gcgctctgcc gggtgaggtg tatttcatca gcaatccgcc
     5881 ggccagcgtt cctgctatgc ccgctgcaat attgccgccg ctgaacagcg cgcccaatgc
     5941 ggacatgctc aacccatgat ccaacaacag cggtgccaga agaggcagca caaaccgcat
     6001 gcctgaattc agcatcaata ccagcagtaa gcccaggcac gcctgcttcc tccttagcgc
     6061 ataacctaac gccggaacat gcgggatagt cgctgttcgc gtcggctccg taatgcgcca
     6121 cagcgggagt gacagcgcca taatcagcac cgccatcatc agcatggcga caggccagcc
     6181 ggatgccgcc gacagcatca ggaacacccc gccgccgcac atcattccca gatagctgcc
     6241 gccgacctgc acgctgtttc cccagccgta acccgtgcgg gtcagttgat cgacacaaaa
     6301 gccgtcgctg acgatatcaa tggtggaggc gaccgtgcct gccaccataa acagaacaaa
     6361 taacgcgacg atttgcgtat cggggaatcc cccttctcgc ccaaaccagc caatcgctgc
     6421 ggcaatagtc aggatcgccg ctagcgcgac ctgaccacgc aggattaaca tgcgggaacg
     6481 gcgttcctgg ctaccgggcg gaagccgcca gcgctcgatc cacggcgccc aaataaactt
     6541 cagcgcccag ggcagcataa acagggttgt cgcaccggca agcgccagcg aaccgccagc
     6601 agcgcgcacc agcgcgggta aggactgcat agacagcgcc gataccagac tctgcgtcac
     6661 atagacgccg gcaatagcaa aaatcacccg cccggtcgtc aacgtcagag gtttcacatt
     6721 cgactgaaca tcactcatgc acccactctc cagacgcgga acccccgtcg tcgcgccaca
     6781 cgcgcgcggc catttgcgcc tgccacagcg cctgataacg tccatgatgt gagagcaatt
     6841 gcgcatgagt accctgctca accacctgtc cctcttccat caccagaatg tttccggccc
     6901 cggcgatggt ggataaacgg tgagcgatga taatcaccgt ccggttgtga accaggttat
     6961 cgatcgcttt ttgcaccgcc agctcgcttt caatatccag cgcggcagtc ggttcatcga
     7021 gaatgacgac cggcgcgttt ttcaataacg ctctggcaat ggaaatccgc tggcgctcgc
     7081 cgcccgatag ctggccgccc atctctccca ttggcgtcag ccagccttgc ggaagacggg
     7141 aaataaactc aaggcactgc gccgcgcggg cggcctcttc tacctcctgc cgcgtcgcct
     7201 gtgggcgcgc gatacggata ttcgccagca gcgtgtcatc aaacagccag acatcctgga
     7261 aaacaacaga aatcaggcta ttgagctgtt ccggcgtcag gcggcgaata tcgacgccgc
     7321 caatagaaat ctgcccttgc tgcggatcgg catagcgcat taacagtttg gtgacggtag
     7381 ttttgcctgc gccggaggca cccaccagcg cgctcatact ggctgccggg aacgtcaaag
     7441 aaacatgatt aagcgcgtgg ccgtcgcctt cttcgtagcg atagctgacg ttgtcaaagc
     7501 ggatatcgta acgttccggc atctcgcttt gctctgcgac gggaagcggt gctatcgcca
     7561 taaactgctc aatccgttgc agggcgctgg cgatcagttc cacaaccgag gtgtagctga
     7621 taaacatcgc catcggttct gcgaagcgca taatcatcgc gacggcggca atcaaaaagg
     7681 cgaggttcag agtgcccgtc actacccaga cgatcccgga taacaccacc acctgtaggc
     7741 ccaactccac gacgctggcg atcagcatcg tcgcgccagc gccctgacgg tgagtgcggg
     7801 tctgtaagtt ttccagcgca ttgaaatgcg ccagcagcgc ccggctttta tcggcatcgc
//
LOCUS       DAYPHN010000192         7805 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000192 DAYPHN010000000
VERSION     DAYPHN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.192"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            51..2087
                     /gene="yihQ"
                     /locus_tag="ACG0BW_004240"
     CDS             51..2087
                     /gene="yihQ"
                     /locus_tag="ACG0BW_004240"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFK7261126.1"
                     /translation="MDTPRPQLLDFQFHQNNDSLTLRFQGRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDATVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2133..3518
                     /locus_tag="ACG0BW_004241"
     CDS             2133..3518
                     /locus_tag="ACG0BW_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261127.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQIGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VTFSCLAFFGSAFVNSLNWALISDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTE"
     gene            3559..4962
                     /locus_tag="ACG0BW_004242"
     CDS             3559..4962
                     /locus_tag="ACG0BW_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261128.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5029..5721
                     /gene="ompL"
                     /locus_tag="ACG0BW_004243"
     CDS             5029..5721
                     /gene="ompL"
                     /locus_tag="ACG0BW_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFK7261129.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5813..7078)
                     /locus_tag="ACG0BW_004244"
     CDS             complement(5813..7078)
                     /locus_tag="ACG0BW_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261130.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7180..>7805)
                     /locus_tag="ACG0BW_004245"
     CDS             complement(7180..>7805)
                     /locus_tag="ACG0BW_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFK7261131.1"
                     /translation="EGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHELVS
                     LVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLGHKA
                     CISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIPYRQ
                     MSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
BASE COUNT         1975 a         1906 c         1850 g         2074 t
ORIGIN      
        1 acaataacag gaaggaaaca acctcgttct caactctatg gagattattt atggatacgc
       61 cacgcccaca gttattagat tttcaatttc atcagaataa cgacagtctt accctacgtt
      121 ttcagggccg tcttatttta acccatagta aagataatcc ttgtttatgg attggctcag
      181 gtatagcgga tatcgatatg ttccgcggta atttcagcat taaagataaa ttgcaggaga
      241 agattgcgct caccgacgct accgtcagcc agtcaccgga tggttggtta atccatttca
      301 gccgtggttc tgacattagc gccacgctga atatctctgc cgacgatcag ggccgtttat
      361 tgctggaact acaaaacgac aaccttaacc acaaccgcat ctggctgcgc cttgccgctc
      421 aaccagagga ccatatctac ggctgcggcg aacagttttc ctacttcgat ctgcgcggca
      481 aaccgttccc gctatggacc agtgaacaag gcgttggtcg caacaagcaa acctatgtca
      541 cctggcaggc cgactgcaaa gagaacgcgg gcggcgacta ttactggact ttcttcccac
      601 agcctacgtt tgtcagcacg cagaagtatt actgccatgt tgataacagt tgctacatga
      661 acttcgactt tagcgccccg gaataccatg aactggcgct gtgggaagac aaagcaacgc
      721 tgcgttttga atgtgctgat acatacatct ccctgctgga aaaattaact gccctgctgg
      781 gacgccagcc agaactgccc gactggattt atgacggggt aacgctcggc attcagggcg
      841 ggacggaagt gtgtcagaag aaactggaca ccatgcgtaa cgcgggtgtg aaggttaacg
      901 gcatctgggc gcaggactgg tccgggatcc gtatgacctc ttttggcaaa cgcgtaatgt
      961 ggaactggaa gtggaacagc gaaaactatc cgcaactgga ctcacgtatt aagcagtgga
     1021 acaaagaggg cgtgcagttc ctggcctata tcaacccgta tgtcgccagc gataaagatc
     1081 tctgcgaaga ggcggcaaag cgcggttatc tggcaaaaga tgcctctggc ggcgactatc
     1141 tggtggagtt tggcgagttc tatggcggcg ttgtcgacct caccaatcca gaagcctacg
     1201 cctggttcaa ggaagtgatc aaaaagaaca tgattgaact tggctgcggc ggctggatgg
     1261 ctgacttcgg cgagtatctg cccaccgaca cgtacctgca taacggcgtc agcgcggaga
     1321 ttatgcataa cgcctggcca gcgctgtggg cgaaatgtaa ctacgaagcc cttgaagaaa
     1381 cgggcaagct cggcgagatc cttttcttta tgcgcgccgg ttctaccggt agccagaaat
     1441 actccaccat gatgtgggcg ggtgaccaga acgtcgactg gagtctggac gatggcctgg
     1501 cgtcggttgt cccggcggcg ctgtcgctgg caatgaccgg acatggcctg catcacagcg
     1561 acattggcgg ttacaccacc ctgtttgaga tgaagcgcag caaagagctg ctactgcgct
     1621 ggtgtgattt cagcgccttc acgccgatga tgcgcaccca cgaaggcaac cgtccgggcg
     1681 acaactggca gtttgacggc gacgcggaaa ctatcgccca ttttgcccgt atgaccaccg
     1741 tcttcaccac cctgaaacct tacctgaaag aggccgtcgc gctgaatgcg aagtccggtc
     1801 tgccggtcat gcgcccgctg ttcctgcatt acgaagacga tgcgcacact tacaccctga
     1861 aatatcagta cctgttaggt cgcgacattc tggtcgctcc ggtgcatgaa gaaggccgta
     1921 gcgactggac gctctatctg ccggaggata actgggtgca cgcctggacg ggtgaagcgt
     1981 tccggggcgg ggaagtgacc gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg
     2041 ccgatagcga atgggcggca ctgttcgcgt cgttaaaaag catctaatca cattcgcccg
     2101 tggggaaact catgggcaaa gggagaacat cgatgagtca catcacaacg gaagatccag
     2161 caactttacg cctgcccttt aaagagaaac tctcttacgg tattggcgac ctggcctcta
     2221 acatcctgct ggatattggt acgctttatc ttttgaagtt ttataccgac gttctggggc
     2281 tacctggtac ctatggcggc attatctttt tgatctcgaa attctttacc gcctttaccg
     2341 atatgggaac cggcatcatg ttggattccc gacgcaagat cggtccaaaa ggtaagttcc
     2401 gtccttttat tctgtatgcg tcattcccgg ttaccttact ggcgatcgcc aactttatcg
     2461 gtacgccgtt cgatgtcacc ggtaaaacgg tgatggccac tatcctgttt atgctttatg
     2521 gactgttttt cagcatgatg aactgttcct acggcgcaat ggtgcctgcc atcaccaaaa
     2581 atcccaacga gcgcgcctca ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc
     2641 tgctgtgtac ggtgggattc gtgccggtta tgaatcttat cgaaggtaat cagcaaattg
     2701 gctatatctt cgctgccacg ctgttttcac tgttcggcct gctgtttatg tggatctgct
     2761 actcgggcgt gaaagagcgt tatgtcgaaa cccaacctac caatccggcg caaaagcctg
     2821 gcctgttgca atctttccgc gcaattgccg gtaaccgccc gctgttcatt ctgtgtattg
     2881 ctaacctctg caccttaggg gcgtttaacg tcaagctcgc cattcaggtt tattacaccc
     2941 agtacgtgct taacgatccc atcctgttgt catatatggg atttttcagc atgggctgta
     3001 ttttcatcgg cgtgttcctg atgcctggcg cagtcagacg ttttggtaag aagaaggtct
     3061 atatcggcgg cctgctgatt tgggtgcttg gcgatctgct caactatttc tttggcggcg
     3121 gttcggtcag cttcgtgacg ttctcctgcc tggcattctt cggctcagcg tttgttaaca
     3181 gcctgaactg ggcgctgatt tccgacaccg tggaatatgg cgaatggcgc accggcgtgc
     3241 gttcggaagg gacggtctac accggtttca ccttctttcg caaagtttct caggcgctgg
     3301 ctggtttctt cccaggctgg atgctgacgc aaatcggcta tgtgccaaac gtcgcccagg
     3361 ctgaccatac catcgaaggt ttacgccagc tgatcttcat ctacccaagc gcactggcgg
     3421 tagtcaccat tgttgcgatg ggttgtttct acagtctgaa cgagaaaatg tacgtccgca
     3481 ttgttgaaga aatagaagcc cgtaaacgca cggaataaat aataaacaac gccctgcggg
     3541 gcgttataag gagtgattat gtctgaccat aatccactga cattaaaact gaatctgcgg
     3601 gaaaaaatcg cctatggcat gggcgacgtc ggttcgaatt taatgcttag catcggcact
     3661 ctgtatctcc tcaaatttta taccgatgaa ttagggatgc ctgcttacta tggcgggatt
     3721 atctttctgg tggcgaagtt ttttaccgcg tttaccgata tgctcaccgg atttttactc
     3781 gattcgcgga aaaatattgg gcctaaagga aagttcaggc cgtttatttt atacgcagcg
     3841 gttccggcgg cgttaattgc aacgctccag tttatcgcca ccacttttag tttgccggtt
     3901 aaaacgacga ttgccacagc actgtttatg atgtttgggc tttcatacag tctgatgaac
     3961 tgctcgtatg gcgcgatgat cccggccatt accaaaaacc cgaatgagcg cgcgcagctc
     4021 gcggcttacc gccagggtgg tgcaaccata gggctattga tttgtaccgt ggcgtttatt
     4081 ccgttgcagt cgcttttttc tgactccacc gtcggttatg cctgcgcggc actgatgttc
     4141 tccattggtg gctttatttt tatgatgctg tgctacagag gcgtcaaaga gcattatgtg
     4201 gacacagcgc caaccggaca taaagccagt attctcaaat ctttttgcgc gatatttcgt
     4261 aatccgccat tgctggtttt gtgcattgct aacctgtgta ccctggcggc atttaatatc
     4321 aaactggcga ttcaggtcta ttacacccaa tatgtgctga atgatattaa tttattgtcg
     4381 tggatgggat ttttcagtat gggatgtatc ctcgtcggcg tattactggt gccattaact
     4441 gtaaaatgtt ttggtaaaaa acaggtttat ttagctggca tggtgctatg ggcggttggt
     4501 gatatcctga attatttctg gggaagtaac tctttcactt ttgtcatgtt ctcttgtgtc
     4561 gccttttttg gcacggcctt tgtgaacagc ctgaactggg cgctggtacc agataccgta
     4621 gattacggtg aatggaaaac cggtattcgt gccgaaggtt ctgtttatac cggttatacc
     4681 ttctttcgta aaatttctgc cgcacttgct ggcttcttgc caggcattat gctgacgcaa
     4741 atcggttatg ttcccaacat cgcgcaaagt gatgcgacat tacagggttt gcgtcagctc
     4801 atttttatct ggccttgtgc gctggcaatt attgctgcat taacgatggg attcttttac
     4861 acactcaatg aaaaacggtt tgcattgatt attgaggaaa tcaaccaacg caaaaataaa
     4921 gagatggcga cagaagaaaa aacggcttcc gtaacgttat aaatgtcaat agccgctatt
     4981 tccatcctgg tgggtggcgg cctccctacg tttaaaaatg gacttattat gaaaaagatt
     5041 aatgcaataa ttctattatc ctctctgacc tctgcctcgg tatttgctgg cgcatacgtc
     5101 gaaaacaggg aggcatataa tctcgcctcc gatcagggcg aagttatgct acgcgtgggt
     5161 tataacttcg atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa
     5221 gatgaactca aacatggcta taatgaaatt gaaggttggt atcctttatt taaaccaacc
     5281 gataaattaa ccatccagcc cggtggctta attaatgaca agagtatcgg ttcgggtggt
     5341 gccgtttatc tggatgttaa ctacaaattt actccgtggt ttaacctgac ggtgcgtaat
     5401 cgctataacc ataataatta cagctctaca gatttgaacg gcgaattgga taataacgac
     5461 acctatgaag ttggcaccta ctggaatttt aaaatcaccg agaaattctc ctatacattt
     5521 gaaccacatt acttcatgcg agtaaatgac tttaatagca gcaatggaaa agatcatcac
     5581 tgggaaatca ctaacacctt ccgttatcgc attaatgaac actggctccc ttatttcgaa
     5641 ttacgctggt tagatcgtaa tgtcgaaccg taccaccgcg agcaaaacca gattcgtatc
     5701 gggacgaagt atttcttctg atgtaaaact gccggatgcg gcgaaaacgc cttatccggc
     5761 ctacggttga tacggtttcc gttggccgga ttaagccaca tccggccatt aatcaagctt
     5821 cagccgccag catctctttc ttacgttggc taatacgctt aaccagcatt acggaaacca
     5881 ccgcaccaca gaatgcgaag caggccataa tgccaaacac aattttgtag ccaataatcc
     5941 ccggatttaa atccagaatg tagccataca gactgaagca gaacatcgcc ggggcgtaac
     6001 caataaagct acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt tttgcttcgc
     6061 cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag cccagcgtac
     6121 atgccatccc taaatatacc ggcatacttt cgtgcggcag cataatcaac agtacgagcg
     6181 cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat
     6241 ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc
     6301 cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga
     6361 atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata agccagatag
     6421 ttttgtcttt cagcaccgag gtcatactgg tgtttttcga tgctccgtct tcttttttca
     6481 cctcaacgga cggtgcctct tctttgtcat tcagaacaaa gaaaatgata atgcctacgg
     6541 caatcacaat aagggaatag aaccagatgc ctgctttaaa acccaataag ccactgccaa
     6601 accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg
     6661 tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga cttaccgact
     6721 tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga
     6781 ggatgcccca atatcccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga
     6841 gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata gacagaaaaa
     6901 agcccactgt cgtgacaaat gagtttaccg acatagcatt accaatttga ccattggtca
     6961 aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa
     7021 ttgtaccgcc acacaatgtt aatagactaa ataacgccca tttctttttc gtgagcataa
     7081 catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc gaccattcat
     7141 atcgaatggt tttattaact gcgtatgagt taaaacttat tagtggttca gaagatggtt
     7201 tgcctcttgt ttgatttgtt gtagtacgtt acggacatga tttatctgat taattgcatc
     7261 ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggcaatg gtgtttcact
     7321 catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga acgccggcgc
     7381 atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat catcacccag
     7441 gcatataagg tgcgttaata acgctttgaa cggcatatca ccctgacctg aaatacaggc
     7501 tttatgaccc aggccaccct cttctttaac gatcaaggca tctttgatat ggacctgggt
     7561 aatatgcggt gccatcgttt ttaaagcgtc gatgggatgc tcatttgcat tgatcatgtt
     7621 cgcaaaatca aacagtaagg aaagagattc catctcactt tctttgacca gcgacaccag
     7681 ctcatggctt tttaaatctt catgctgttc gatcgtaaaa gtcaggccgc tgtcctgata
     7741 cgtttcccgt acataggcaa tgtcgttagc gatgatcgat aatacgtcgc gcaaattacc
     7801 ttcat
//
LOCUS       DAYPHN010000193         7784 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000193 DAYPHN010000000
VERSION     DAYPHN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.193"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..2775
                     /locus_tag="ACG0BW_004246"
     CDS             <1..2775
                     /locus_tag="ACG0BW_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFK7261132.1"
                     /translation="TNLTDAVDSLGDDSLLWNATAGAFSAKHGTNGTDSKITNLLAGT
                     VSSDSTDAINGSQLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAA
                     INTSFSTSLGDALLWDATAKGGDGAFSAGRGTDNTASIITNVADGAISSTSSDAINGS
                     QLYDTSKYIADTLGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWD
                     ATANDGNGAFSAAHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGD
                     AEVNADGSITAPTYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKAS
                     IITNVADGNIGEGSTDAINGSQLFNTNMLIQQNSEIINQLAGNTSETYIEDNGAGINY
                     VRTNDNGLAFNDASASGIGATAVGYNAVASGESSVAIGQGSSSSVDTGIALGSSSVSS
                     RVIVKGSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTV
                     RQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNVGIGIGYGAYVD
                     ANALNGIAIGSNASANHANSIAMGSGSQTTRGAQTDYTAYNMDAPQNSVGEFSVGSED
                     GQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGI
                     GDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTIS
                     VGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSVDYSNITLGGGNG
                     GTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIAS
                     AMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEY
                     SAALGAGIQW"
     gene            3186..4841
                     /gene="lldP"
                     /locus_tag="ACG0BW_004247"
     CDS             3186..4841
                     /gene="lldP"
                     /locus_tag="ACG0BW_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFK7261133.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4841..5617
                     /gene="lldR"
                     /locus_tag="ACG0BW_004248"
     CDS             4841..5617
                     /gene="lldR"
                     /locus_tag="ACG0BW_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFK7261134.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5614..6804
                     /gene="lldD"
                     /locus_tag="ACG0BW_004249"
     CDS             5614..6804
                     /gene="lldD"
                     /locus_tag="ACG0BW_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFK7261135.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            6877..7350
                     /gene="trmL"
                     /locus_tag="ACG0BW_004250"
     CDS             6877..7350
                     /gene="trmL"
                     /locus_tag="ACG0BW_004250"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFK7261136.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7407..>7784)
                     /locus_tag="ACG0BW_004251"
     CDS             complement(7407..>7784)
                     /locus_tag="ACG0BW_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7261137.1"
                     /translation="ACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSA
                     LADRGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQ
                     KRIKLSLGGLSPVEYRTEHQKAG"
BASE COUNT         1758 a         2065 c         2074 g         1887 t
ORIGIN      
        1 accaacctga ctgacgctgt tgacagtctc ggtgacgatt ccctgctgtg gaacgctacg
       61 gcgggtgcat tcagtgccaa acacggcacc aacggtactg acagcaaaat caccaactta
      121 ctggcaggca ctgtatcctc tgatagcact gacgctatta atggctcaca actttatggc
      181 ttagctgatt catttacgtc ataccttggc ggtggtgctg atatcagcga tgcgggtgta
      241 ttaaccgggc caacctacac tattggtggt actgattaca ataacgtcgg tgatgctctg
      301 gctgccatta acacgtcatt tagcacatca ctcggtgacg ccctactctg ggatgcgacc
      361 gccaagggcg gtgacggtgc gtttagcgcc ggtcgcggga cagataacac agccagcatc
      421 attactaatg ttgcagacgg tgcaatttcc tctaccagca gcgacgctat taacggctca
      481 cagctctatg acaccagcaa gtacattgcc gatactctgg gtggtgacgc agaagtcaat
      541 gctgacggca caatcaccgc accgacttat gccatcgctg gcggcagtta cagcaacgtc
      601 ggtgacgcgc tggaagcgat cgataccacg ctggatgacg ctctgttgtg ggatgcaaca
      661 gccaatgatg gcaatggtgc atttagcgcc gctcacggaa aagataaaac agccagtgta
      721 atcactaacg tcgctaacgg tgcaatctct gccaccagca gcgatgccat taacggttca
      781 caactgtata ccaccaacaa gtacattgcc gatgccctgg gtggtgacgc agaagttaac
      841 gctgatggtt ctattactgc gccgacttac accattgcaa atgccgagta caacaacgtc
      901 ggtgacgccc tggatgcgct cgacgataac gctctgctgt gggatgcaac agccaatgac
      961 ggcgcaggtg cctacaacgc cagccatgac ggcaaggcca gcatcatcac aaatgttgct
     1021 gatggtaaca ttggcgaagg cagcactgac gccatcaacg gttctcagct gtttaacacc
     1081 aatatgctga tccagcagaa cagcgaaatc atcaatcagc ttgccggtaa caccagcgaa
     1141 acctacatcg aagataacgg tgcgggcatt aactatgtac gtaccaacga caacggctta
     1201 gcgttcaacg atgccagcgc ttcaggtatt ggcgctacgg ctgtgggtta taacgctgtc
     1261 gcctcaggcg aaagcagcgt agccattggt caaggtagca gcagctccgt tgatacgggt
     1321 atcgccctgg gtagcagttc tgtttccagc cgtgtaatag ttaaaggttc tcgtgacacc
     1381 agcgtgtcgg aagaaggtgt tgtgattggt tatgacacca cggatggcga actacttggt
     1441 gcgttgtcta ttggtgatga cggtaaatat cgtcaaatca tcaacgtagc cgatggttcc
     1501 gaagcccatg acgccgttac ggttcgccag ttgcaaaatg ccattggtgc agtcgctacc
     1561 acgccgacca aatacttcca cgctaactca acagctgaag attcactggc agtaggtgaa
     1621 gactcgctag caatgggcgc gaaaactatc gttaatggta atgtgggtat tggtatcggt
     1681 tatggtgcct acgtggacgc gaatgcactt aatggcattg ctattggtag caacgcgagt
     1741 gcaaatcatg caaacagtat tgcgatgggt agtggttccc agaccacccg tggtgcgcaa
     1801 actgattaca ccgcctacaa catggacgca ccgcagaatt ctgtcggtga attctctgtc
     1861 ggcagtgaag acggtcaacg tcagatcacc aacgtcgcgg ctggttcagc ggataccgat
     1921 gcggttaacg taggtcagtt gaaagtaacg gacgcgcagg tttcccagaa tacccagagc
     1981 attactaacc tgaacaatca ggtgacgaat ctggatactc gcgttaccaa tatcgaaaac
     2041 ggcattggcg atatcgtaac caccggtagc accaaatact tcaagaccaa caccgatggc
     2101 gtagatgcca acgcgcaggg taaagatagt gttgcgattg gttctggttc cattgctgcc
     2161 gctgacaaca gcgtcgcgct gggtacaggc tccgtggcca acgaagaaaa taccatctct
     2221 gtgggttctt ctaccaacca gcgtcgtatc accaacgttg ctgcaggtgt taatgccacc
     2281 gatgcggtta acgtttcgca gctgaagtct tctgaggcag gcggcgttcg ttacgacacc
     2341 aaagctgatg gctctgtaga ctacagcaac atcactctcg gtggtggtaa tggcggtacg
     2401 actcgcatca gcaacgtctc cgctggcgtg aacaacaacg acgcagtgaa ctatgcgcag
     2461 ctgaagcaaa gcgtgcagga aacgaagcaa tataccgatc agcggatggt tgagatggat
     2521 aacaaactgt ccaaaaccga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     2581 accggtctgc cgcaggctta cactccaggt gccagcatgg cctctattgg tggcggtact
     2641 tacaatggtg aatcggctgt tgctttaggt gtgtcgatgg tgagcgccaa tggtcgttgg
     2701 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc gctcggtgcc
     2761 ggtattcagt ggtaatcatc cattaacgaa tgggtaaacg ccacattgcc tgatgcgcta
     2821 cgcttatcag gcctacgggg tgattcagca cattcctgta ggttggataa ggcgttcacg
     2881 ccgcatccgg caagataagg cgcaccagtt caacaatctg gggcaattct ctgatgggaa
     2941 ttgccctttt cttcattacc tctctcccaa gattggccct accaattctt cgcttatctg
     3001 acctctggtt cacagatctc caattaaaac tcacatcaat gttgccaata cataacattt
     3061 agttaaccat tcattgtcat tatccctaca caacacaatt ggcagtacca cttttacaca
     3121 acgtgtgaca aggagatgag catcagactc attacacgat gtgcgtggac tgcaggagac
     3181 ctgcaatgaa tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc
     3241 tgatagcatc gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactcaaag
     3301 gttatgtcgc cgcctcatgg acggtggcaa tcgcccttgc cgtggctttg ctgttctata
     3361 aaatgccggt cgctaacgcg ctggcctcgg tggtttatgg cttcttctac ggtttgtggc
     3421 ccatcgcgtg gatcattatt gcggcagtgt tcgtctataa gatctcggtg aaaaccgggc
     3481 agtttgacat catccgctcg tctattcttt cgataacccc tgaccagcgc ctgcaaatgc
     3541 tgatcgtcgg tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgctccgg
     3601 tagcaattac cgccgcattg ctggtcggtc tgggctttaa accactctac gccgccggac
     3661 tgtgcctgat tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccgattctgg
     3721 tcgccgggca ggtaacaggc atcgacagct ttgagattgg tcagatggtg gggcgtcagc
     3781 tgccgtttat gaccattatc gtgctgttct ggatcatggc gattatggac ggttggcgcg
     3841 gaatcaaaga aacgtggcct gcggtcgtgg ttgcaggtgg ttcgtttgcc attgctcaat
     3901 acctcagctc taacttcatt gggccggaac tgccagacat tatctcttcg ctggtatcac
     3961 tgctctgcct gacgctgttc ctcaaacgct ggcaaccagt gcgcgtattc cgcttcggtg
     4021 acttgggggc gtcacaggtt gatatgacgc tggctcacac cggttacacc gcaggtcagg
     4081 tgcttcgcgc ctggacaccg ttcctgttcc tgaccgccac cgtaacgctg tggagtattc
     4141 caccgtttaa agccctgttc gcttcgggtg gtgcgctgta tgaatgggtg atcaatattc
     4201 cggtgccgta cctcgataaa ctggttgccc gtatgccgcc agtggtcagt gaagcgacag
     4261 cgtatgccgc cgtgtttaag ttcgactggt tctctgccac cggtaccgcc attctttttg
     4321 ctgccctgct ctcgattgtc tggctgaaga tgaaaccatc tgacgctatc agcaccttcg
     4381 gcagcacgct gaaagaactg gctctgccta tctactccat cggtatggtg ctggcgttcg
     4441 cctttatctc gaactattcc ggactatcat caacgctggc gctggcactg gcgcacaccg
     4501 gccatgcatt caccttcttc tcgccgttcc tcggctggct tggtgtcttc ctgaccggat
     4561 cggatacctc atctaacgcc ctgttcgccg ccctgcaagc cactgcagca caacaaattg
     4621 gcgtttctga cctgttgttg gttgccgcca acaccaccgg tggtgtcacc ggtaagatga
     4681 tctctccgca atctatcgct atcgcctgtg cggcggtagg cctggtaggc aaagagtcag
     4741 atctgttccg ctttaccgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca
     4801 ccacgcttca ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg
     4861 cctgtcagac gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaggc
     4921 gggcatgaag ttgcccgctg aacgtcaact ggcgatgcaa ctcggcgtat cacgtaattc
     4981 actgcgcgag gcgctggcaa aactggtgag cgaaggcgtg ctgcttagtc gacgcggcgg
     5041 cgggacgttt attcgctggc gtcatgacac atggtcggag caaaacatcg tccaaccgct
     5101 gaaaacgctg atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc
     5161 cattgaagcc agcaccgcgt ggcatgcggc aatgcgcgcc acaccaggcg aaaaagagaa
     5221 aattcagctt tgctttgaag caacgctaag tgaagacccg gatctcgcct cacaagcgga
     5281 cgttcgtttt catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat
     5341 gcgcggtttc ttcgatgtcc tgcaatcctc tgtgaagcat agccgccagc gaatgtatct
     5401 ggtacccccg gttttttcac agctgaccga acaacatcag gcagtcattg acgccatttt
     5461 tgccggtgat gctgacgggg cgcgtaaagc aatgatggcg caccttagct ttgttcacac
     5521 caccatgaaa cgattcgatg aagatcaagc tcgccatgca cgcattaccc gcctgcctgg
     5581 tgaccataat gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat
     5641 cgcgccgcag cacaacgcat tctgccgccg ttcctgttcc actatatgga tgggggtgca
     5701 tattctgaat acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc gctgcgccag
     5761 cgtattctga aaaacatgtc tgacttaagc ctggaaacga cgctgtttaa tgagaaattg
     5821 tcgatgccgg tggcgctggc tccggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa
     5881 gttcaggctg ccaaagcggc ggatgcgcat ggcattccgt ttactctctc gacggtttcc
     5941 gtttgcccga ttgaagaagt cgctccagcc attaaacgtc cgatgtggtt ccagctttat
     6001 gtgctgcgcg atcgcggctt tatgcgtaac gcactggagc gagcaaaagc ggcaggctgc
     6061 tcgacgctgg ttttcaccgt ggatatgccg acaccgggcg cgcgttaccg tgatgcacac
     6121 tcgggtatga gcggcccaaa tgcggcaatg cgccgttact tacaggcggt gacgcatccg
     6181 caatgggcgt gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcggct
     6241 tacctcggca aaccgaccgg actggaagat tacatcggct ggctggggaa taatttcgat
     6301 ccgtccatct catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg
     6361 atcaaaggga tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga
     6421 attgtggttt ctaaccacgg tggccgccag ctggacggtg tgctctcttc tgcccgtgca
     6481 ctgcctgcta ttgcggatgc cgtgaaaggt gatatcgcca ttctggcgga tagcgggatt
     6541 cgtaacgggc ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt
     6601 cgtgctttcc tgtatgcgct ggcaacagcg ggccaggcgg gcgtggcaaa tctgcttaat
     6661 ctaatcgaga aagagatgaa agtggcaatg acgctgactg gcgcgaaatc gatcagtgaa
     6721 attacgcaag attcgctggt gcaagggctg ggtaaagagt tgcctgcggc actggctccg
     6781 atggcgaaag ggaatgcggc atagttgggg gtgaggggaa aaggccgtac caatcaccaa
     6841 ttttctgcta tcctgccccc gcactaaggg ggcagtatgc taaacatcgt actttacgaa
     6901 ccagaaattc cgccaaatac tggcaacatc atccgtcttt gcgctaatac cggctttcgt
     6961 ctgcatatca tcgaaccgat gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg
     7021 ctggactatc acgagtttac cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa
     7081 gcagaaaatc cccagcgcct gtttgccctc accacgaaag gtacgcctgc tcacagcgcc
     7141 gtaagctatc aggatggcga ttatctgatg ttcggcccgg aaacgcgcgg cctgccagca
     7201 agcattctcg atgccctgcc cgctgaacaa aaaattcgca ttccaatggt gccggacagc
     7261 cgcagcatga acctgtccaa tgcggtgtcg gtcgtggtgt atgaagcctg gcggcagctg
     7321 ggatatccgg gagcggtgtt gagagattag ttactgtatg ccggatagat gatttgaact
     7381 gaaccccaaa acctggacag tttcagttaa ccggcttttt gatgctcagt ccggtattct
     7441 accggactga gtcccccaag actgagtttt attctttttt gattccagta agttatgtat
     7501 tcgttaacgg cattttccag ctcttctaca tttctgtagt cccggcgata gtacatttct
     7561 tctttcaggt gaccaaagaa gttctccatt accgcattat caaggcagtt gcccttgcgc
     7621 gacatgctct gcactaaccc tctgtccgcc agggcggact gataactttt tatccggtaa
     7681 tgccatcctt gatcactgtg gagcagcggt ttctcccctt cagcaaggct ttccagcccc
     7741 ttatttaaca tccgcttcac cagctcctct gtaggcctgc aggc
//
LOCUS       DAYPHN010000194         7779 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000194 DAYPHN010000000
VERSION     DAYPHN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.194"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            470..1180
                     /locus_tag="ACG0BW_004252"
     CDS             470..1180
                     /locus_tag="ACG0BW_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HFK7261138.1"
                     /translation="MSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQS
                     PVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIID
                     TPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGS
                     LLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPR
                     KWRFDATNSELCAERHEC"
     gene            1107..3229
                     /locus_tag="ACG0BW_004253"
                     /pseudo
     CDS             1107..3229
                     /locus_tag="ACG0BW_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3399..4451)
                     /locus_tag="ACG0BW_004254"
     CDS             complement(3399..4451)
                     /locus_tag="ACG0BW_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFK7261139.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPVVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4766..6082
                     /gene="shiA"
                     /locus_tag="ACG0BW_004255"
     CDS             4766..6082
                     /gene="shiA"
                     /locus_tag="ACG0BW_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFK7261140.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATTLIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            6184..7638
                     /gene="amn"
                     /locus_tag="ACG0BW_004256"
     CDS             6184..7638
                     /gene="amn"
                     /locus_tag="ACG0BW_004256"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFK7261141.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2122 a         1825 c         1741 g         2091 t
ORIGIN      
        1 ccaacaaaca atcctatata caaggcacat ctccagaata taaaagcaca gacaaacaac
       61 ctaaaaaaaa caaccccata catataaatt attttatagg taaaatagat tatatattct
      121 catagctacc acaaactata ctagcctgaa ctatatttat tctgctgcaa tcaatgcata
      181 cataacacaa atatcactca ggtacactac tcaaaccacg ctgggatttt tcctcaagtt
      241 ataatcatca ccccgaaaat cattcggcat ttactcatta aatagtcacc ccataggcct
      301 gtacatgttt actcagaaat atacatcctt ttctctgtca taaaccctct aattaatcat
      361 aaataaatac ttgtgacacc aatctttttc cttaacggaa cgaattgttg tgtagaagga
      421 gataatatta tgtcaaaaaa aatatcagcc attgcttata actcattata tgtcaacatg
      481 ggtcactata acggaagcag ttgagatcac taccaaagcc ataaaacaaa aaattactcc
      541 tagcgatatt tatcgtcatg ccttgagtgg caacatccta ctatcggtct atttccagtc
      601 tcctgtgata cttaagaaga tacaaacttt taatggaaaa ataaaattca ggcagtttgt
      661 gggagaccta cttgataaac tatgcatgct tgacagggac ggttttattt atggtcaaaa
      721 tttaagactc tgtacagaag ctagatatat ttgtcctgtg caacaaatta tagatacccc
      781 cctgttacgc aaactcaacc agtttcgtac gtttgttcgt aatgtccgcc cgggtgatga
      841 actggatgtc ccggcacaag ttagtgaaaa aaatttaacc ccgccgccgg gtaacagcag
      901 tggcaacctt gagcaacaga tagccagtac ttcacagcta attggatctc tgctcgccga
      961 agatatgaac agcgagcaag cggcaaacat agcacgtgga tgggcctctt ctcaggcttc
     1021 aggggtaatg acagactggt taagccgctt cggtaccgca agaatcacgc tgggcgtgga
     1081 tgaagatttc agcctgaaga actcccgtga cggcaaccct cgaaaatggc gattcgatgc
     1141 aacaaacagt gaactatgtg ccgaacgtca cgaatgctga aatcacgctg gcagcctcga
     1201 aggacccggt gattgccgac aataacgatc tcacgacact aacagcaccg tcgctgatac
     1261 agagggcaat gcaatagcca acactgaggt aacatttact ctaccggaag atgtgaaggc
     1321 gaacttcacg ctgagcgatg gcgacaaagc ggttactgat gccgatggca aagcgaaagt
     1381 cacgctgaaa ggtacaaaag caggcgctca taccgtgaca gcatcgatgg ttggcggtaa
     1441 gagtgagcag ttggtggtga actttactgc ggatacgctc actacgcagg ttaatcttaa
     1501 cgttaccgag gacaatttca tcgccaataa cattggaatg accaggctac aggcaacagt
     1561 gactgatgga aacggcaacc cagtagaagg tataaaagtt aatttccgtg gaacctccgt
     1621 cacgctaagc agcaccagcg ttgaaacgga tgatcaggtt ttcgctgaaa ttcttgtgac
     1681 aagcaccgag gtcggactaa aaacagtttc agcctctctg gcagataaac ctactgaagt
     1741 catctcgcga ttactgaatg ccaaggtaga tgttaattct gcgacgatta ccagtcagga
     1801 gatacctgaa ggtcaggtca tggtcgcaca agacatagca gttaaagctc acgttaacga
     1861 ccagtttggc aacccggtga cgcatcaacc cgcgacattc agtgcagcgc catcctcgca
     1921 aatgatcatc agccagaata cggtctctac taatacgcag ggagtagccg aggtcaccat
     1981 gacgcctgaa agaaacggct cgtatactgt aaaagcatcc ctagcgaatg gagcttcact
     2041 tgagaaacaa ctggaggcta ttgatgaaaa actgacactc acgtccagtc cgcttatcgg
     2101 tgtcaatgct cctaaaggcg ctactctgac ggcaatgcta acctctgcaa atggcactcc
     2161 agtggagggt caggtcatca actttagcgt aacgctagaa ggtgcgacgt taagtggcgg
     2221 aaaagtgaga accaactctt caggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
     2281 tacatatacg gtgactgcat cgttccataa cggcgtcaca atacagacac agacaaccgt
     2341 gaaggttact ggcaacccaa gcaccgcaca tgttgctagc tttatcgctg aaccgtcgac
     2401 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
     2461 taacctgatc gaaggtctca ctgtgtactt cgccttaaaa aacggctcca ccacattaac
     2521 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
     2581 aacggggagc gtcactgtaa gcacagtcac aagcgcaggt ggaatgcaaa cagtagatat
     2641 atcgctagtg gcaggcccag cagacgcctc gcagtccatc cttaagaaca accagtcatc
     2701 actgaaaggg gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
     2761 caatccgatc aaagtttctg aagggatgga atttgtgcaa tcaggtacaa acgtgcccta
     2821 tatgaaaatt agcgcaattg attacactca aaatatcaac ggtgattaca aagctactgt
     2881 tacaggagac ggagagggta tcgcaacgct gatccccgta ttgaatggtg ttcatcaggc
     2941 gggcctgagt accacgatag aattcattag cgcggaaacc agaccaatga cgggtacagt
     3001 gtccgtgaat agtgcaaacc taccgacagc ttcattccct tcgcagggat ttaccggggc
     3061 gtattatcag ttgaataatg acaactttgc accaggaaaa acggcggctg attattcgtt
     3121 ttcaagctcg gcctcctggg taggcgttga tgctactggt aaagtgacat tcaaaaatga
     3181 cggtgatagc aatacagtaa taatcacagc gcccccaaga tccggtggtg ctatttacca
     3241 aacagttcct cctgaatcgc gttcggtgtg actatgatca ttttcggtga gtattgcttt
     3301 gatccaaagg ctggttttct gtgatgattg accagcctca gagtattgat gacaccaccg
     3361 ttcataacag atcggcatta ctatgcataa gtactgcctc aaattctcgc caaaacactt
     3421 tttaatgcct ttgcgaattc atccagactt atattgtcaa ttttttgccc aacgacgact
     3481 gactccgaaa catcggccat tgcgggccat cgcgttttgt aatcagcata aattgcaaca
     3541 acaggggtat tataggcact tgccatatgt aatattgagg tgtcaggagt tattgcaata
     3601 taagcatctt ttatgattgc tgctgaacgt ttaatcgagg gtgataagga taaacggtag
     3661 acattattat aacaatccac taatgccctg gctttatctt cccctttggg tccataaacg
     3721 atcactattg gaatatctgt ttcactttga atttttgcta ttagattttc tgcaatcgat
     3781 aatgaaaatg tacgttcctg cgagctaccg tctaaattga gcgcaatgta agatcctaac
     3841 gatcgcattt cctcgcgtac ctcatcgagt acatcatcgc ttaatggcaa ttcatatatt
     3901 gacctgacta ccggaaaacc cgcctcacgc atcataaatg cccatgtcat gggtacagga
     3961 gtccggagat tctgatccat ccgggacgcg ttcttacaca aaggggaata gcacttcatg
     4021 gttaaaccga cgacctggaa attcgttttt gctttcaact gactgataaa tatcatcgtt
     4081 ttcaggttct ttttacgcat cgcctctata caaagatccg gcgtaccgta ttgcagtgtt
     4141 atattcttcg ctaaatcttt tatttctttt aatgttgcgt gatcctgcat agtcattgtg
     4201 actaatgtta atttagtctg ttcaagtttg agcgcattaa agacttctaa attaattgtc
     4261 gacgtaacaa ttaatagatg cttaatttta tgcaattcaa gcgcccgaat aacaggaaag
     4321 atggccatag catcgccaat ctgatcggga atatgtatga caacaaagtc tgttttttca
     4381 atattaaaat tataagcttt ataatcgtag taactaaatg caatacgtct caacaatgat
     4441 actaaaaaca taactaacct cgcctcccta ctggttataa tgcaatgcag tctatcagaa
     4501 tcatcagggt gcaattttgt gcatatgcgg acttttatgt ttcatatctc gaacctgtgg
     4561 gccatctgct taatccttaa acaacaccag caactcctgc gctttcatct tccatcgaat
     4621 ttttcatgtt gccgctaatc agccataaaa tcatttgcag atgcgctctg tcgaggtagt
     4681 ctcataaggt tcgtttatag atcgacggca atgtgagtta ccttttccat actaattata
     4741 aaaagacagt acaaacagga tcattatgga ctccacgctc atctccactc gtcccgatga
     4801 agggacgctt tcgttaagtc gcgcccgacg agctgcgtta ggcagtttcg ctggtgccgt
     4861 cgtcgactgg tatgattttt tactctatgg cattaccgcc gcactggtgt ttaatcgcga
     4921 gtttttcccg caagtaagcc cggcgatggg aacgctcgcc gcatttgcca cctttggtgt
     4981 cggcttcctt ttccgaccgc tcggcggtgt cattttcggt cactttggtg accggctggg
     5041 acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc atcgcgacaa ccttgattgg
     5101 tattcttcct tcattctcga ccattgggtg gtgggcacct attttgctgg tgacactgcg
     5161 tgccattcag ggatttgccg tcggcggcga atggggaggc gcagcgttgc tttccgttga
     5221 aagtgcgccg aaaaataaaa aagcctttta cagtagcggt gtacaagttg gctacggagt
     5281 aggtttactg ctttcaaccg gactggtttc attgatcagt atgatgacga ctgacgtaca
     5341 gtttttaagc tggggctggc gcattccttt cctgtttagc atcgtactgg tactgggagc
     5401 attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc aacataatca
     5461 agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc ccggtgcttt
     5521 cctgaagatt attgcgctac gactgtgcga gttgctgacg atgtacatcg ttaccgcctt
     5581 tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc ttaatattgg
     5641 tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc ttgccgatcg
     5701 ttttggtcgc cgcagggttt atatcacagg tgcgttaatc ggaatgttga gcgcatttcc
     5761 tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct ccataatgct
     5821 ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt
     5881 tggtgccagt tatcgctata gtggcgctgg agtcggttat caggttgcca gtgtggttgg
     5941 tggtggattt acacctttta ttgccgctgc actcatcact tactttgccg ggaactggca
     6001 tagcgtcgcc atttatttgc tggctggatg cctgatttcc gcaataaccg ctttgttgat
     6061 gaaagacaat caacgcgctt gatagcctgg cgaagatcat ccgatcttcg ccttacactt
     6121 ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca
     6181 cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg
     6241 ctgtatgagc aatctgtagt tgctttacgc aacgccattg gcaactatat tacaagtggc
     6301 gaattacctg atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc actgaccgta
     6361 acttgggatg gtagcacaac caatccccct aaaacgcgcg catttggtcg ttttactcac
     6421 gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta tctcaatgaa
     6481 caacttacgt tgctgtatca ggattatggt gcgcatatct cggtgcaacc ctcgcagcat
     6541 gaaatccctt atccttatgt cattgatggc tctgaattga cacttgatcg ctcaatgagc
     6601 gctgggttaa ctcgctattt cccgacaaca gaactggcgc aaattggcga tgaaactgca
     6661 gacggcattt atcatccaac tgaattctcc ccgctatcgc attttgatgc gcgccgcgtc
     6721 gatttttccc tcgcgcggtt gcgccattat accggtacgc cagttgaaca ttttcagccg
     6781 ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc
     6841 cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg gaactggatc
     6901 accgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag
     6961 atgcccgcgt ggcatttaat taccgccgat ggtcagggta ttactctggt gaatattggc
     7021 gtgggaccgt caaatgctaa aaccatctgc gatcatctgg cagtactacg cccggatgtc
     7081 tggttgatga ttggtcactg tggtggctta cgtgaaagcc aggccattgg cgattatgta
     7141 cttgcacatg cttatttacg cgatgatcac gttcttgatg cggttctgcc gcccgatatt
     7201 cctattccaa gcattgctga agtgcaacgt gcgctttatg acgctaccaa gttagtcagc
     7261 ggcaggcccg gtgaggaagt caaacagcgg ctgcgtacag gtactgtggt aaccacagat
     7321 gacaggaact gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc
     7381 gtggcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca
     7441 tacgggacac tactgtgtgt ttcagataaa ccgttgcatg gcgagattaa acttcccggc
     7501 caggctaacc gtttttatga aggcgctatt tccgaacacc tgcaaattgg cattcgggcg
     7561 atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg aacctttaat
     7621 gagccgccgt tccgataata agttatgaac cttcttatca gaaatagatg tgaaggatta
     7681 agtaagacat ctggcaaata gcctgcaata aacgcgggct gtttgcttcc ttgggcagat
     7741 gcgagtttta ttatcgtctt aatgatttcc acatattaa
//
LOCUS       DAYPHN010000195         7569 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000195 DAYPHN010000000
VERSION     DAYPHN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.195"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACG0BW_004257"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACG0BW_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFK7261142.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACG0BW_004258"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACG0BW_004258"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFK7261143.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACG0BW_004259"
     CDS             complement(941..1408)
                     /locus_tag="ACG0BW_004259"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFK7261144.1"
                     /translation="MDAALPFLILYACLSTLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACG0BW_004260"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACG0BW_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFK7261145.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACG0BW_004261"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACG0BW_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFK7261146.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACG0BW_004262"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACG0BW_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFK7261147.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACG0BW_004263"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACG0BW_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFK7261148.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACG0BW_004264"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACG0BW_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFK7261149.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACG0BW_004265"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACG0BW_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFK7261150.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACG0BW_004266"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACG0BW_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFK7261151.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACG0BW_004267"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACG0BW_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFK7261152.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACG0BW_004268"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACG0BW_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFK7261153.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACG0BW_004269"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACG0BW_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFK7261154.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACG0BW_004270"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACG0BW_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFK7261155.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACG0BW_004271"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACG0BW_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFK7261156.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..>7569
                     /gene="rplX"
                     /locus_tag="ACG0BW_004272"
     CDS             7290..>7569
                     /gene="rplX"
                     /locus_tag="ACG0BW_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFK7261157.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKV"
BASE COUNT         2056 a         1767 c         1983 g         1763 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagtttcgaa ttaaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgcaatttca ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtcttaat gacgtcgaat
      601 accatgaatc tatcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagtt ggatctcatc ggtaaaatcg
      901 gtctacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccagcc tacaacgaaa cccgcagccg ccagaaatgg accaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc ataagcaaac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccatgtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatggcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacttgatag aacaaaagac ctgaccagag taacgggcag gtaaacctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggttgataaa caagcgtaca
     1381 aaatcagaaa gggaagggca gcgtccataa aatatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggta
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgtt gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg atgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcacgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggta
     7561 aaaaagtcc
//
LOCUS       DAYPHN010000196         7456 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000196 DAYPHN010000000
VERSION     DAYPHN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.196"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            93..1244
                     /gene="yiaY"
                     /locus_tag="ACG0BW_004273"
     CDS             93..1244
                     /gene="yiaY"
                     /locus_tag="ACG0BW_004273"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFK7261158.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1445..2983
                     /gene="aldB"
                     /locus_tag="ACG0BW_004274"
     CDS             1445..2983
                     /gene="aldB"
                     /locus_tag="ACG0BW_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFK7261159.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQRLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            3261..3446
                     /locus_tag="ACG0BW_004275"
     CDS             3261..3446
                     /locus_tag="ACG0BW_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFK7261160.1"
                     /translation="MKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKD
                     IKPGTLAQIGRQAGLTF"
     gene            3463..3873
                     /locus_tag="ACG0BW_004276"
     CDS             3463..3873
                     /locus_tag="ACG0BW_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFK7261161.1"
                     /translation="MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALV
                     AHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWLLVDINMNQFEGRAERINITMPK
                     RLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV"
     gene            complement(3945..5909)
                     /locus_tag="ACG0BW_004277"
     CDS             complement(3945..5909)
                     /locus_tag="ACG0BW_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFK7261162.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPLRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLCLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEETSA"
     gene            complement(5920..7320)
                     /locus_tag="ACG0BW_004278"
     CDS             complement(5920..7320)
                     /locus_tag="ACG0BW_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261163.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSHWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
BASE COUNT         1814 a         1897 c         1984 g         1761 t
ORIGIN      
        1 attaattttg ataaacaata atactgatca tattttcgaa tgaaaatcct cagtaagctg
       61 cccgcccttt tttacacttt caggagtgtg ttatggcagc ttcaaccttt tttattcctt
      121 ccgtgaatgt tattggtgct gattcattga atgatgcaat gaatatgatg gcagattatg
      181 gatttacccg taccttaatt gtcactgaca atatgttaac gaaattaggt atggcgggtg
      241 atgtgcaaaa agcactggaa gaacgcaata tttttagcgt tatttatgat ggcacccaac
      301 ctaacccaac cacggaaaac gtcgccgcag gtttgaaatt acttaaagaa aataattgcg
      361 atagcgtgat ctccttaggc ggtggttctc cgcacgactg cgcaaaaggt attgcgctag
      421 tggcagccaa tggcggcgat attcgcgatt acgaaggcgt tgaccgctct gcaaaaccgc
      481 agctgccgat gatcgccatc aataccacgg cgggtacggc ctctgaaatg acccgtttct
      541 gcatcatcac tgacgaagcg cgtcatatca aaatggcgat tgttgataaa catgtcactc
      601 cgttgctttc tgtcaatgac tcctctctga tgatcggtat gccgaagtca ctgaccgccg
      661 caaccggtat ggatgcctta acacacgcta ttgaagcgta tgtttcaatt gctgcaacgc
      721 cgatcactga cgcttgcgca ctgaaagcgg tgaccatgat tgccgaaaat ctgccgttag
      781 ccgttgaaga tggcagcaat gctaaagcgc gtgaagcaat ggcttatgcc cagttcctcg
      841 ctggcatggc attcaacaac gcatctcttg gttatgttca tgcgatggcg caccagctgg
      901 gcggtttcta caacctgcca catggtgtat gtaatgccgt tctgctgcca cacgttcagg
      961 tgttcaacag caaagtcgct gctgcgcgtc tgcgtgactg tgctgctgcc atgggcgtta
     1021 acgtgacagg caaaaatgac gcggaaggcg cagaagcgtg catcaatgcc atccgtgaac
     1081 tggcgaagaa agtgaatatc ccggcaggcc tgcgtgacct gaacgtgaaa gaagaggatt
     1141 tcgcggtact ggccaccaat gccctgaaag atgcgtgtgg gtttactaac ccgattcagg
     1201 caactcacga agagattgtg gcaatttatc gcgctgcgat gtaatcatca ttcccccaat
     1261 ggctggctaa ttgtcagcca ttttttaaac tatctcaaaa tttgcgattt ttgagagagt
     1321 tttagttatc tctgtaaccc ttgcccgtaa attcgtgata gccgtcgtaa agctgttacc
     1381 gactggtgaa gatttcgcca gtcacgtcta cccttgttat acctcacacc gcaaggagac
     1441 gatcatgacc aataatcccc cttcagcaca gattaagccc ggcgagtatg gtttccccct
     1501 caagttaaaa gcccgctacg acaactttat tggcggcgaa tgggtagccc ctgccgacgg
     1561 cgagtattac cagaacctga cgccggtgac cgggcagcgg ctgtgcgaag tggcgtcttc
     1621 gggcaaacga gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc
     1681 gcatacctcg gtgcaggatc gcgcggcgat tctgtttaag attgccgatc gaatggaaca
     1741 aaaccttgaa ctgttagcga cggctgaaac ctgggataac ggcaaaccca ttcgcgaaac
     1801 cagtgctgct gatgtaccgc tggcgattga ccatttccgc tattttgcct cgtgtattcg
     1861 ggcgcaggaa ggcggtatta gtgaagtgga tagcgaaact gtggcttatc acttccacga
     1921 accgttaggc gtggtggggc agattattcc gtggaacttc ccgctgctga tggcgagctg
     1981 gaaaatggct cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac
     2041 cccgctttct gtactgctgc taatggaaat cgtcggtgat ttactgccgc cgggcgtggt
     2101 gaacgtggtc aacggtgcag gtggggaaat tggcgaatat ctggcgacct cgaaacgcat
     2161 cgccaaagtg gcgtttaccg gctcaacgga agtgggtcaa caaattatgc aatacgccac
     2221 gcaaaacatt attccggtga cgctggagct gggcggcaaa tcgccaaata tcttctttgc
     2281 tgacgtgatg gatgaagaag atgccttttt tgataaagcg ctggaaggct ttgcactgtt
     2341 tgcctttaac cagggcgaag tttgcacctg tccaagccgt gctttagtgc aggagtctat
     2401 ctacgaacgc ttcatggaac gcgccatccg ccgtgttgaa agtattcgta gcggtaaccc
     2461 gctcgacagc gtgacgcaaa tgggcgcgca ggtttctcac gggcaactgg aaaccatcct
     2521 caactacatt gatattggta aaaaagaggg cgcagacgtg ctcaccggcg ggcggcgcaa
     2581 gctgctggaa ggagaactga aagacggcta ctacctcgaa ccgacgattc tgtttggtca
     2641 gaataatatg cgcgtgttcc aggaagaaat ttttggtccg gtgctggcgg tgaccacctt
     2701 caaaacgatg gaagaagcgc tggagctggc gaacgacaca caatatggcc tgggcgcggg
     2761 tgtctggagc cgcaacggta atctggccta taagatgggg cgcggcattc aggctggacg
     2821 cgtgtggacc aactgttatc acgcttaccc ggcacatgcg gcgtttggcg gctacaaaca
     2881 gtcgggcatc ggacgcgaaa cccataagat gatgcttgag cattaccagc aaaccaagtg
     2941 cctgctggtg agctactcgg ataaaccgtt ggggctgttc tgatataaga agctggtcgc
     3001 attgggtatt cattgcctga tgcgacgttt gcgcgtttta tcaattcaaa tgtctacgtc
     3061 ttgaatgact gaatattgat tgaacttaat tacgagtctc ctcgcatatt ttctaccaat
     3121 tactgcacac cgttattctt cttccgagtg gcattcctga gtcaacttta tccccctgac
     3181 ggcccatata aatcgcgcta tcgtgattgt tatcgctaat tgtgtgtgta tcgtgtgtat
     3241 aatcatggaa gggcagggat gtgaaaagtg cagacgtgat cgccatcctg aagcagcatg
     3301 gctgggaaca tattcgaacc cgtggcagtc atcatcagtt ccgccaccct gttcatccgg
     3361 gtctggtgac ggttccgcac cctaaaaaag atattaagcc cggtacgctg gcgcaaattg
     3421 ggcgtcaggc tggtttaaca ttttaaaatt tcaggaggca atatgtttta tcccgcctat
     3481 atccacagtg atcctgacgg cagcgccagc ggttttttcc ctgatgttcc aggctgttat
     3541 tttgctggtg atacgctgga caacgctttc caggatgcgc gaagtgcact ggtcgcgcac
     3601 ttcgaaacct tatgcgagat gaatgaagag ttgccgcttc caggctctgt ggaaacacat
     3661 ctggtccagc gtgcgcagga ctttattggc gggcaatggt tgctggtgga tatcaatatg
     3721 aatcagttcg aaggtcgggc tgaacgtatc aatattacga tgccgaaaag gttactgaac
     3781 aagattgata cctacgtgcg caataaccca gactacgcca acagaagtgc gtttctggca
     3841 gaagctgccc ggcgtgtatt gccgggcgtt taatctcgcg tttcgatggt tagcgggcaa
     3901 ccgcaccgat atgttgatac tgataagacg cgttaagcgt cgcatcatgc gctggtttcc
     3961 tcattcaccc agatccgcat ttcgccttca ccccggttag cccagctaaa ccacgggata
     4021 aacgtcagag tttgcggctg gcgtttggct ggcgcactgt cgtaatgcca cagcggttgc
     4081 tgctctggat tgctctgttc ataccggtaa cccggtgcct ggattaagat cttatggctg
     4141 aacaatccct tgccttcaaa ggtcgtaaat ggcgcatcgg cgggcagcca caggttatgc
     4201 agtgactcgc cgttgtcggc ctgttccagg caatacacca gcgggccgcg ctgaatcgcc
     4261 acttttccgg cgacgtgacg caccagaggg ttaccgtaaa cgcggcgtac cggcatcggc
     4321 aaagtcagat tcagcgtatc gccctcctgc cattcgcggg taatgtgcaa atacccttta
     4381 cgaatatcct gctcgacctc ttccccattc aatatgatct gcggctgtgt gcaccagtcc
     4441 ggcagacgta aagccagcgt atgacgcacc ggctggggcg attcaaccgc aattgtcacc
     4501 tgttcctgcc acggatagtt cccgctaacc cgcaggcaca acgtgccatt ttctaccggc
     4561 acttccatgc tgtttcctgc gtagatgttg atatacaacg catcttcacg cggcgtgtag
     4621 agataatgac caatcgaggt cagtacgcgg gcgatatttg gcggacaaca agcgcagcca
     4681 aaccaacgct ggcggagcgg tttaacgtga tcgtagatat ggttgaattt cagcgatttt
     4741 ggatgtactt ccagcggatt cacatagaag aaatgtttgc catccagtgc catgccgccg
     4801 agcacagtgt tgtacagcgc gcgctccatc acatcggcat attgactgtc gccttccatt
     4861 tccagcattc gccgggcaaa catcatcagg ccgatggagg cgcaactttc agcgtatacc
     4921 gtgtcattcg gcagatcgta atcgctgctg aacgcttcac cgctgctttg tgagccaatg
     4981 ccgccggtaa tatataactg acgctgggcc atattgttcc acagccgcag gcaatcctga
     5041 cgcttgcttt catcgtgact taaacgcgcc agatgtgcga cgccagtcat caggtagaca
     5101 aagcgtactg catgtccgat ggcggtttgt tgctgtgcga tgggcaaatg cgcctggctg
     5161 taggctttat ctttcaccat ccacgccggg ccgtaggtgt gccagtgcga ggtctgcccg
     5221 cgcttttcat attcctggtc gtaatagtgc ggttgcgcac cacgctgttc gacaaaatag
     5281 ttcgtcagcg ccaggtagcg cggctcttcg gtcacttcat acaggcgcat tagtgccagt
     5341 tcaatttccg ggtgaccagg ataaccgtgt aacttacttt cacctggacc aaatacgctg
     5401 tcgatatgat cggccagacg gcaaaccact tccagcaagc gccgcttgcc cgtggcctgg
     5461 aagaaggcga ctccggcttc aatcagatga ccggcgcagt aaagttcatg acactccgcc
     5521 agattgctcc agcgttcttc gggcgctttt accgtaaagt aagtattgag atagccatct
     5581 tcgcactggg cggaggcgat cagttcaatc acctcgtcag cagttttttc cagttcggca
     5641 tccggcttct ggcacagcga ccaggctacc gcttccagcc atttggcgac gtcgctgtcc
     5701 tgaaacacca tcccgtaaaa ttcaccgtct tgcagtcctg cggcaatgcg aaagttttca
     5761 atcgcatggc tgggttccgc ttctgggata cgatcgttca aggcgtccca ctgataagga
     5821 atcaccacat cgcggaccag ttgttggtac tgaccgagga acggatcgct gaccgtcagt
     5881 ttatgcagat cgacttccga aatgttcatc ttgtgctcct taaggctgta catgacgctg
     5941 acgcaggtcg ctagcaatgc gtgacatcat agggttattg agtttgcaaa agcggatcgc
     6001 catcgccagc agcaggtgga atagcgcagg cagcaatgtt tccattgctg tgatcccgcc
     6061 aagcgaactg gcggtctggt tttccacgcc tggctggtag gcgacaaaaa taaacagcag
     6121 gctgatgatc ccggcgctgg aggcccaggc cagcttgatg aaaaacagat tgaaggcgaa
     6181 gttcatgccg gaagaacgta cgcgggtttt ccactcgccg tagtcatcgg caaaggccat
     6241 caatgagaag tgcagtggca atgtgaagcc aagaatcacg ccattaccga ggatcaccgc
     6301 cagccacagc gtttgataag ccgggccaga gggcaggaac cacatcaaca ccgccagcgc
     6361 agcgaggagc aggttggtgt agtagtaaat tttgacggta tcgaaacgtc gcgttaacgg
     6421 gctgacaatc accgagccga taatggaggc gaaggtgacc atagtgaaga acagcgacgt
     6481 gtagcccgta ctgccttgta agacgtaggt aatgaagtac atatacccac cgccgcgaat
     6541 attaaagacg ttaatcagca ggaaagacat gaccagcatc agcagcagtt ggtcgttgtt
     6601 tcgcagcccg gcaagatgtt cgcgcagggt aaatttcccc attgtggaga gtggtacccg
     6661 ttcacgaacc cagaagaaac agcacaggaa catcaccaca gcgatggcgc acagcacgcc
     6721 gacgcccagt tgatagccgc gcgcggcatt accctgaccg aagagatcca ccatccacgg
     6781 taagcctacc gaaaccagaa atcccgctac accgcagaga acgaatcgcc aggactggca
     6841 ggagatcact tcattgtggc gggtggtcat ggtgttgatc agcgcgcagt acggcacgtt
     6901 gatggcggta taaccgactg aaaggagcag gtaagtgccg aacgcccagg cgatttttac
     6961 tcccatgctg gcatctggca cggtgaacgt caggatgccg ataatgccga tgggaatggc
     7021 aatccataac tgccacggac gaaagcgccc ccagtgactt tgcgtccggt cggcaatgac
     7081 gcccatcacc gggtcggaga tggcatcaaa cacgcgcagt gcaataaaca gcgtgccgac
     7141 cagcgccgga gttaagccaa aaacgtcggt gtagaaaaag gtgagaaagt tcatgatcag
     7201 gcaagttatt acggtgccgc ctgcgtcacc caggccgtag cctatttttt cccgtacaga
     7261 taacgcatcg tcaattttac ctttagcgat atcagcggta gtaatcggtg tagaggtcat
     7321 agatattcct cgtgaatagg gagcaatttt tagttttatt gcaatgacct gattaattct
     7381 gccgtgaacg ggaatatcaa caaggcgaga aaagagtaaa aaaaggtgac gattccgacc
     7441 tgaatttaaa agtgtg
//
LOCUS       DAYPHN010000197         7281 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000197 DAYPHN010000000
VERSION     DAYPHN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.197"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(157..792)
                     /gene="msrQ"
                     /locus_tag="ACG0BW_004279"
     CDS             complement(157..792)
                     /gene="msrQ"
                     /locus_tag="ACG0BW_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFK7261164.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVVIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            complement(793..1797)
                     /gene="msrP"
                     /locus_tag="ACG0BW_004280"
     CDS             complement(793..1797)
                     /gene="msrP"
                     /locus_tag="ACG0BW_004280"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFK7261165.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRECPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1906..2319)
                     /gene="hiuH"
                     /locus_tag="ACG0BW_004281"
     CDS             complement(1906..2319)
                     /gene="hiuH"
                     /locus_tag="ACG0BW_004281"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFK7261166.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2452..3123
                     /gene="hprR"
                     /locus_tag="ACG0BW_004282"
     CDS             2452..3123
                     /gene="hprR"
                     /locus_tag="ACG0BW_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFK7261167.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3123..4481
                     /gene="hprS"
                     /locus_tag="ACG0BW_004283"
     CDS             3123..4481
                     /gene="hprS"
                     /locus_tag="ACG0BW_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFK7261168.1"
                     /translation="MKRLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRILITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKLNVFRITLPQ
                     RN"
     gene            complement(4589..5440)
                     /gene="hchA"
                     /locus_tag="ACG0BW_004284"
     CDS             complement(4589..5440)
                     /gene="hchA"
                     /locus_tag="ACG0BW_004284"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFK7261169.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6031..7224)
                     /gene="ompC"
                     /locus_tag="ACG0BW_004285"
     CDS             complement(6031..7224)
                     /gene="ompC"
                     /locus_tag="ACG0BW_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFK7261170.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFLNGRANGVATYRNNGFF
                     GQVDGLNFALQYQGNNEGSGGLWDQEGSGNGNNRKLSKENGDGFGMSTSYDFDFGLSL
                     GAAYSNSDRTDNQRATGLGEQRSNKYAGGETAEAWTVGAKYDANNVYLAAMYAETRNM
                     TAYGSGKDNNDIDRGGIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGWAHDGN
                     GDPRYTNKDLVKYVEVGATYYFNKNMSTYVDYKINLLDNDDDFYEANGIATDDIVAVG
                     LVYQF"
BASE COUNT         1985 a         1590 c         1709 g         1997 t
ORIGIN      
        1 ggcgtattga actatcttat tgaaataaaa taaattattc tggtgatgaa aggttgatga
       61 caaacgaaac ataacttgat gcgatctgtg tgggccggat agggcgctcg cgccgcatcc
      121 ggccgggata acgcgaagtt tgtcggcaat atgaagttat agagataatt tgttgtgaac
      181 ctgcttgcgt aaccggttga aaagggaaag caacttctta taccgtaagg ctaaaagcag
      241 taccgccagc ccagcgtaga tgagtggctg cggtgaaata atcttcactg accacagata
      301 atgtattggg gccaggatca cgacaagata gacgaagtta tgcaactgtt gccagtgttt
      361 gccgagtttt cgctgcatcg cctgggttga agtgaacgct aaagcaagca aaataaccca
      421 gctgataatt cctaacgtta aataaggccg ggtaattaac tccttaccta ataacgccag
      481 attattcacg cccagctcca gtaatgcgta actggttaaa tgcagtgtcg cccaggcaaa
      541 gcaccataag cctaacaggc ggcgagtgcg tatcaataac ggctgttttg cgtagcgtgc
      601 cagaggtgtg attaacaagg tcgccagcaa aaatttcagt gcagtgcgac cagtaaaatg
      661 ctgaatatct ttcaccggat cggcacccaa tccaccgtga ttaatcgccc agaccagcca
      721 gagaaacggc aacaatccgg caagatgcag gcaaactttc agccatgtca cctgttttgc
      781 tgtcagacgc attcagaaat tctcccgcaa atccaggcca cgatacagcg atgccacctg
      841 gtcggcgtaa ccattaaaca gtagcgttgg ctggcgctgt acatcgagga tgccgcctga
      901 accaataaat cgttcggtag cctgtgacca gcgcgggtga tcaacatgcg gattaacgtt
      961 ggcgtaaaaa ccgtattcgt caggcgctgc cagattccag gtggttggcg gacactcgcg
     1021 ggtcagctta atactgacta tcgatttaat ccctttaaag ccatatttcc acggcacaat
     1081 cagtcgcacc ggcgcgccat tttgtggcgg taacgccttg ccataaacac ccacggtcat
     1141 cagtgtgagc ggatgcattg cttcgtcgag acgcaatcct tcgacataag gatatttcag
     1201 cccgccgccg ataaagcggt cctgctggcc aggcatctgt tccggtgcat aaattgtttc
     1261 gaaagcgaca tacttcgcat tgctggtggg ttcggcaagc gccagcaatt tgtgcagcgg
     1321 aaaaccaatc cacggcacca ccatcgacca tgcttccacg cagcgcatac gataaatacg
     1381 ctcttccagc gggaagcgac gggttaaatc atcgtgatcg agggtcaatg gttttgccac
     1441 ttcgccgctg attttcaatg tccatggatc ggttttcagg ctaccagcat tagcggcggg
     1501 atcggcttta tccagcccga attcatagaa gttgttataa ccggagactt tatctgctgg
     1561 cgtcagtggc aggtcatttt gccaggcggc aggcttgctg aactccaacg gttttccggc
     1621 gggggcgggt ggacgatcat tccctttaaa ccagctaagc agatcggcat gcgcagcgtg
     1681 aggcaaagaa agtgcagccg cgctgatgcc cagtgctttt aacacctgcc gacgcttcat
     1741 aaagaatacc gactcggccg taacatctga ttcttttaaa aattgattct ttttcataag
     1801 ccatcacact tttttgtcgt tagccagcaa gagccgttta ctgttaatct gtcagggagc
     1861 cacctcccta acagtagcag agatctgctt ttatatattt atgcattaac tgccacgata
     1921 ggttgaatac ccatattggc taagtaataa aggcacatga taatgctcgt tcactttatt
     1981 gatatgaaat tcaacgggga tctcagggaa gaaactttca agattttgtt tcttgaaata
     2041 gtccccggtt ttaaatacga cacggtaatc gcccgtagtt gcagtttgct cgggccacag
     2101 tgccttaatt cgtccatcct tatctgtttt ggcggtatta agttgtaacc agccgttgtc
     2161 cgctttcttt tcaagagtga ctgtcacgtc ggcagcgggt tttcctgttt gctggttcaa
     2221 aatgtgcacg ctaagaatgt tttgttgtgc agcatagacc aaagaaggta atgaaaatgc
     2281 agccgttact acggagagta ctaaataacg ctttaacatg tttatatcct tgtcatgtga
     2341 atgagttttg aggatagcga aaaaacgtcc agggtaacct tacagcagca ttacaatttt
     2401 gtaatgaacc aggctgtttc tataacatat gatttatggc atattatttt catgaagatt
     2461 ttacttattg aagataatca aaggacccag gagtgggtaa cgcaggggct ttccgaagcg
     2521 ggttatgtca ttgatgccgt ttctgatggc agagatgggc tttatcttgc gctgaaggat
     2581 gattatgcat tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta
     2641 caaacgttaa gaacagcaaa gcaaacccct gttatttgcc ttactgcaag ggattctgtc
     2701 gatgacagag tcagagggct ggacagtgga gcaaatgatt atctggtaaa acctttttca
     2761 ttttctgagt tgctggcaag ggttcgggca caattaaggc aacatcatgc tttgaattca
     2821 acattagaaa tcagtggctt aagaatggac tctgttagtc aaagtgtgag cagggacaat
     2881 atcagtatta cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct
     2941 ggcgaaatta tacccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt
     3001 gataccaata cggtggacgt cgccattcgc aggctccgcg caaaagttga tgatcctttt
     3061 cctgaaaagc taatcgccac aatccggggg atgggctatt cattcgtagc ggtaaaaaaa
     3121 taatgaaaag attatctata accgtccgtt taaccttgct ttttatattg gtgctgtctg
     3181 ttgctggcgc tggaattgtc tggactctct ataatggcct ggcaagtgag ttgaaatggc
     3241 gcgatgatac aacactcatt aaccggacag cgcagatcaa gcagttgtta attgatgggg
     3301 taaatccaga tacgttacct gtgtacttta accggatgat ggatgttagt caggatatct
     3361 tgatcattca tggtgatagc atcaacaaaa ttgttaaccg gacgaatgtc agtgatgaca
     3421 tgttaaataa catacctgct actgagacaa tcagcgcagc tggcatttac agaagcatta
     3481 ttaatgatac agagatagat gctttacgaa ttaatattga tgaagtttcg ccatcattaa
     3541 cggttactgt ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatta
     3601 atagcattat aatttgcatt gtcgccatta tactttgctc agtattaagt ccgctgttaa
     3661 tcagaacggg gttacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata
     3721 acgataaccg ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc
     3781 aggcgttgaa taaaatgcac caagccttag tcaaagattt tgaacgccta agtcaatttg
     3841 ctgacgatct cgctcatgaa cttagaacgc caattaatgc attactgggt cagaatcagg
     3901 ttacgctcag tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggaaacattg
     3961 aagagctgga aaatatttcg cggttaacag agaacatact gtttcttgcc agggcagata
     4021 aaaataatgt tttggtgaaa ctggactcgc tttctctcaa taaggaagtc gaaaatttgt
     4081 tggactatct tgaatacctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc
     4141 agcaaatttt tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaacg
     4201 ctattcgata ttcaccagaa aaatcgcgca ttcttataac cagttttctt gataccaacg
     4261 gctctcttaa tattgatatc gccagccctg gaacgaaaat taatgagcct gaaaaactct
     4321 tccgtagatt ttggcgcgga gataattcgc gtcattccgt aggtcaggga cttggtcttt
     4381 ctttagtcaa agcgattgcc gaattgcatg gaggaagtgc tacgtatcac tatctcaata
     4441 agcttaatgt gttccggatt acgttaccgc aaagaaatta acatagggtt tcagtacgcc
     4501 ataaattaac cgtgaaaaaa cgcagccacg tcgtgagtac taacgcagct gcgattgatt
     4561 atgcgcttac attcaaacgt aacagggatt aacccgcgta agctgccagc atttcctgcg
     4621 ccgccagttt acccaacgca ttcgctgcga aaggactgtc gccggtgaga actttacggt
     4681 ccttatgtac tcgcccggtg atgtcgtcat taataatgtt catgcccatt ttcttcagct
     4741 cttcgccgaa gtaccaggtg agatgacccg gcatatagcc tatctctggc gtttgtttgt
     4801 ctgcggcgtc tgggaatgcg caaatggaat aaccattcag tgggttatcg ccgtggcgaa
     4861 gcgccagaaa agccgccggg ccgtggcaaa gggagataac aaaacggtca tttttgattg
     4921 cccactgtaa agcggcagcc acgtcctggc tttcaggtaa accaataagt gcgccatgac
     4981 caccaggaac aaagattgct gcatattcgc tgtctgcgtt gaggctggcg acaacatccg
     5041 cgagtttctt cggattgcgg aacaacgatt tatgctgctc aaagaatggc attacttttt
     5101 catctttgtg cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca
     5161 cttcgaattc gaaacctgca gcgtggagat gatacaacgg cagcaacgtt tcaatcggat
     5221 ggttaccggt cgagaacagt tttccgttat cggtcggcaa ataacgttcg tccgcggcga
     5281 tcaccagaat tttatgttta ccgcgatacg gttttggata gtccacgcca tcaagatcag
     5341 agacaggact ggtatattgg ctaagcgaat attctgaagg gaagaatgca ttatcttcag
     5401 caatatcgac ctgcggattt ttacttgttt gaacagtcat agtgtattcc ttattgttgc
     5461 ttagttaggg tagtcactat atttgcgact gagctggtcg ctatttttaa ccagtctgga
     5521 aaattgagtc aatatatcga aagcggtagt cggcgggtgt aagtatgtaa tgatgatgct
     5581 gcactggtgt tgaatattat tcacgccaaa ttttccttat tgaacaacgt cgtcgcaagt
     5641 ggcctcaatg attttagacc attatcagag gtccggggcg gcagggagag atttagtgct
     5701 gtggaatatt gtggtgctga tgaatattta tatcccggat tggcagagaa aaagcttttt
     5761 tcatgcacta tttttgcaaa tattcctata tggacggcgg ttttgttgcg gattaattca
     5821 taaagaaatg cttttgggtg ttggctggaa gagacgctgg tagattttat tgagccaggg
     5881 gggcggggta aggtgctgta tcgatatgct ttggcaattg cgtgtgccgc agatggagat
     5941 gaaatgccaa taaaaaagcc tgagttcgat gagggacgaa ctcaggctgg tgttaagttt
     6001 tacagtaaca cgccgcggcg cgttggctga ttagaactgg tagactaagc ccacagcaac
     6061 gatatcatcg gtagcgatgc cgttagcttc gtagaagtcg tcgtcgttgt ccagcaggtt
     6121 gattttgtaa tcaacatagg tggacatatt tttgttgaag tagtaggtcg caccgacttc
     6181 aacgtattta accagatctt tattggtgta acgcggatcg ccattaccgt catgagccca
     6241 accgcctaaa tccttccctt tagattgcag gtaagcgatg gacgggcgca ggccgaagtc
     6301 gaactgatac tgagcaacaa cttcaaagtt ttgggttttg ttcgcaatac cacctctgtc
     6361 aatgtcgtta ttgtcttttc cgctgccata tgcagtcata ttacgtgttt ctgcatacat
     6421 tgccgctaaa tagacattat tcgcatcata tttagcaccg actgtccatg cttcagctgt
     6481 ttcaccacca gcatatttat tcgagcgttg ctcacctaaa ccagtagcac gttggttatc
     6541 agtacggtca gagtttgaat atgcagcccc gaggcttaac ccgaagtcaa agtcatagga
     6601 agtggacata ccgaaaccgt caccattttc tttagacagt ttacgattat taccattacc
     6661 agaaccttct tgatcccaaa gcccccccga tccttcatta ttaccctgat attgcagagc
     6721 aaagttcagg ccatcgacct gaccaaagaa gccattgtta cggtaggttg caacaccatt
     6781 agcacgaccg ttcaggaagt tatcggcacc agcataggta tcgccaccaa attctggcag
     6841 catatcggtc catgcttcga tgtcatatac gacaccgtag ttacgaccat agtcgaaagt
     6901 accattctga ccaaagccta aacctgcaaa tgccagacgc gttgcggtat tacctttgtc
     6961 gctttcagga ccgtttgcgc caatgttaaa ttcccactga ccataaccag tgaattggtc
     7021 agcgatctga gtttcacctt tgaaaccgat acgggcatat gacatgtcgc catcgctgcc
     7081 agcatcatca gagaagtagt gcaggcccgc tacttttccg tacaaatcca gtttattacc
     7141 atctttatta tagacttcag ccgcatttgc tgcgccagca actaataacg ccgggaccag
     7201 cattgccaga acttttcttt tcattatgta ttcccttgtg attataatct tcatgaatat
     7261 atcaataagt gccgttatcc a
//
LOCUS       DAYPHN010000198         7267 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000198 DAYPHN010000000
VERSION     DAYPHN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.198"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(51..335)
                     /gene="ppnP"
                     /locus_tag="ACG0BW_004286"
     CDS             complement(51..335)
                     /gene="ppnP"
                     /locus_tag="ACG0BW_004286"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFK7261171.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(407..1084)
                     /locus_tag="ACG0BW_004287"
     CDS             complement(407..1084)
                     /locus_tag="ACG0BW_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFK7261172.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1342..1533)
                     /gene="yaiA"
                     /locus_tag="ACG0BW_004288"
     CDS             complement(1342..1533)
                     /gene="yaiA"
                     /locus_tag="ACG0BW_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFK7261173.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1583..2107)
                     /gene="aroL"
                     /locus_tag="ACG0BW_004289"
     CDS             complement(1583..2107)
                     /gene="aroL"
                     /locus_tag="ACG0BW_004289"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFK7261174.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2290..2748)
                     /locus_tag="ACG0BW_004290"
     CDS             complement(2290..2748)
                     /locus_tag="ACG0BW_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFK7261175.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQLPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2868..3677
                     /gene="proC"
                     /locus_tag="ACG0BW_004291"
     CDS             2868..3677
                     /gene="proC"
                     /locus_tag="ACG0BW_004291"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFK7261176.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3694..4809)
                     /gene="adrA"
                     /locus_tag="ACG0BW_004292"
                     /gene_synonym="dgcC"
     CDS             complement(3694..4809)
                     /gene="adrA"
                     /locus_tag="ACG0BW_004292"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFK7261177.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4911..5231)
                     /gene="psiF"
                     /locus_tag="ACG0BW_004293"
     CDS             complement(4911..5231)
                     /gene="psiF"
                     /locus_tag="ACG0BW_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFK7261178.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5350..6765)
                     /gene="phoA"
                     /locus_tag="ACG0BW_004294"
     CDS             complement(5350..6765)
                     /gene="phoA"
                     /locus_tag="ACG0BW_004294"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFK7261179.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6865..7125)
                     /gene="iraP"
                     /locus_tag="ACG0BW_004295"
     CDS             complement(6865..7125)
                     /gene="iraP"
                     /locus_tag="ACG0BW_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFK7261180.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT         1757 a         1916 c         1733 g         1861 t
ORIGIN      
        1 gagagaacat ttcagaaaaa tgccctcccg ttcaagggga aggcgaggaa ttacagatag
       61 cggcacagat aagaggtggg ttcggcaact tgcagatgaa actcactgtg accgggaaca
      121 ttaaacaccg aaccggcttc atacacttgc cagtcggtcg cgtcaggcag taacacattc
      181 agcgcgccac tgattaccgt catctcttcc ggctcagcgg tgctgaaggt gtattcgcct
      241 tcaaccataa cacccacgct ggcgcgacca gtgctgctgc tggaaaagcc gatcgatttc
      301 actttgccgg aaaagtactc attactttga agcataaact ggccctgtct ggtagttgaa
      361 catctacttc actatagggg ccagaggcgc tggctgtcac gcaaaattac accagtaatt
      421 ccgcagccag ccgtgcaatc aatacgttag agagcaagac aggaacatcg agctgttttt
      481 gcagtaaatc gcgatgacgc tggttaaatc ccaaacaatc cagcatgatg acatctgccc
      541 cttttgccag taattctttc ccggcatcaa tgattttttg ttctgaatca tgaatggggt
      601 tacccaatga aaatactggc ggtttctgca aaatttgcca tttttgcgcc tgaacggtca
      661 gcaactcctc aaccggtacg ataaccccca cctgatgatc ttcaacaata gaggaaacca
      721 gcggtggcaa tatccgcgac ggctcaagaa agatcgtatt acgcgcagtc atactactaa
      781 tgtttgctgt actcattaat aaaatgacgt cataaccctg attatcgagc acttcaacca
      841 caccttgcag gtctcgttcc actttacgac gcgaaacatg ggccagctgg ttgtcattta
      901 ataatgtgag aatggtgtct tcgcctgctt ctggcgcgta ctccgccatc acttcttcac
      961 gacttaactt ccccagcagg ctatgatggg aaatattatc ttcgtcaatg tattctgtca
     1021 ggagcggcaa aacttcctgc ataggtacaa tgccgatggt caggatcgcc aacgacgcac
     1081 tcattgttct tccaccttac ttttatttcg tcgttaatta ccgggcaagc gctaagcacc
     1141 actgctgaca cttacctgtg ctacaaatga caaaaagcgt agcagcagac ctgcacggca
     1201 taatgtaaag attccaaatg attccagtaa tggattgtta ttgtttaata ttctaattat
     1261 ttgaaaaaca tgaattgtga aaaatgtgac gcagatccct tgtggtggca ctcagaatcc
     1321 cttccgagca gtctgatgcg gtcactcttt gtcagggtcc tcatagcgtt ttttcgcatc
     1381 catcgcttcc tgagcggtcg gatgttcact gatcaacgag tctggtttag gatgatcggc
     1441 tctgagttgg taccaggtta ccgtctgtcc tggttttcct ttttcaatcg tcactatata
     1501 tgcttcacga ggataaggcg gtttcgttgg catcgttctt ccttatttca cgggatgaac
     1561 gttaagtata ggcgctcgaa aatcaacaat tgatcgtctg tgccagggca ctgcgaattt
     1621 cagaaatcac ctggctgggt tcgtttgttg cgtcgatgat aatatgcgca acttcgcgat
     1681 atagcgcatc gcgttcttcc agcacttcct gaacttcttc gctcagcggt ttccccgtta
     1741 aggttggccg taaatcttct tccggtgcag cttgcagtcg gttaaccagg actgatactg
     1801 gcgcacacaa ataaaccacg atcccgttat tttgcataaa gtgacgatta aattccgtca
     1861 gaataatgcc gccgcccgta gcgataacgg tggatggcgc agttaccgct tccagcgccg
     1921 ccgtttctct ggcgcggaat cccgcccact cttccctttc gacgatctcc gcgaccgtca
     1981 tattgagctg tgattgcaac cactggtcgg tatcgacaaa tcgacggtta agcgaatcgg
     2041 caagggccat tccgaccgtt gttttaccac agccccgagg cccgatcaga aaaagaggtt
     2101 gtgtcatcgt gggttttccc caataggtcg caatgcggcg aaagccggtg tcatgagaat
     2161 agcgatcata ccatcaaact agtacaattt cgattgtaaa gaaaaaattc cacttaaagt
     2221 gaaaatttta atacatccct tactatacca ataaatattc aagaatgaag tgtaaataat
     2281 aaattacatt cagccacgac tacgttgcac ttccagccac cacttctcca gctccgcggc
     2341 aaaggcctgg cggtcacgtt gtgaaagact atctggtccc ccggtctgga tcccactggc
     2401 gcgtaaggta tccataaaat cgcgcatcgt cagacgctca cgaatggtcg ctggagtgta
     2461 acgttcgccg cgtggattaa gcgccgccgc gcctttctcg atggcttcag cagccaaagg
     2521 aatatctgcg gtgatcacca aatcgcccgc ttcacactgc cggacaattt cgttatcggc
     2581 aacgtcgaaa cctgccgcga cgcgcagcgt acgaataaat cgcgatggcg gcacgcgtaa
     2641 actctggttt gctaccagta ccagcggcaa ctgcatacgt tcagccgcgc gatacaaaat
     2701 ctctttaatt acattgggac acgcgtcggc atccacccaa attgtcataa agtcatcctt
     2761 tgttgggtaa tcctctattg tgtcgcgctt ttgccttccg gcatagttct gtttatgctt
     2821 ctgccagcga ttatcaaaac aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa
     2881 tcggttttat tggctgcggc aatatgggaa aagccattct cggcggtctg attgccagcg
     2941 gtcaggtgct tccagggcaa atctgggtat ataccccctc cccggataaa gtcgccgccc
     3001 tgcatgacca gttcggcatc aacgccgcag aatcggcgca agaagtggcg caaatcgccg
     3061 acatcatttt tgccgccgtt aaacctggca tcatgattaa agtgcttagc gaaatcacct
     3121 ccagcctgaa taaagactct ctggtcgttt ctattgctgc aggtatcaca ctcgaccagc
     3181 ttgcccgcgc gctgggccat gaccggaaaa ttatccgcgc catgccgaac actcccgcgc
     3241 tggttaatgc cgggatgacc tccgtaacgc caaacgcgct ggtaacccca gaagataccg
     3301 ctgatgtgct gaatattttc cgctgctttg gcgaagcgga agtaattgcc gagccgatga
     3361 tccacccggt ggtcggtgtg agcggttctt cgccagccta cgtctttatg tttatcgaag
     3421 cgatggccga cgctgccgtg ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg
     3481 ctcaggcggt aatgggttcc gcaaaaatgg tgctggaaac gggagaacat ccgggggcac
     3541 tgaaagatat ggtctgctca ccgggaggca ccaccattga ggcggtacgc gtactggaag
     3601 agaaaggctt ccgtgccgca gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa
     3661 aactcagtaa atcctgatga cttttgccgg acgtcaggcc gccacttcgg tgcggttacg
     3721 tccggctttc tttgctttgt aaagcgccaa atctgccgat ttcaaccact cacgatagtg
     3781 actcatttgt gggttcagcg gcgcaacccc cacactaatc cgtaaagtta cctgtggcgt
     3841 attcggcaga cgtaatgtat ttagcccttc atgcactcgt aacatggcgg taatggcgct
     3901 ctcagctggc gtaccggaca tgattaccgc aaactcatcg ccgccaaacc gaccaatcac
     3961 atcgctaccg cgcagggtta tttgtaattg tcgggtaagc gccacaatcg cttcatcgcc
     4021 cacatcatgg ccccaggtat cgttgatgct cttgaaatgg tcgatatcga taatcagtaa
     4081 tgtcgcatcg cggttatgcc gccgacagtt atcaaattca ttgcgtaaca tagtttccca
     4141 atgacgtcgg ttatacacgc ccgtcatacc gtcgcgggta ctcatgacct gcaacctgcg
     4201 tttatgttcc gccagtttag tcgccgtctg gtagctgacc cagccaaaca gcagaggata
     4261 aatgacgata atgggaaggg agagccacca ttccagcggc gcactattga acgacacggt
     4321 gatgcccgtc agttcaaggg tgacaagaca ggaaaccacc atcaacacca gacccgcgac
     4381 aaacagacgg gggccgcctg cccccatcaa attcagacac ataatcatca acatcgcggt
     4441 ggaaggcagc acgtttacgc ccattacgcc tacccacatt cccgctaata ctgcatcggt
     4501 ttttaagttg taaatttccc ggctaagcgg atcgacggcc ctgctcgcta tctgccaggc
     4561 taaatgcggc cagacgaacg cccagccgac caacaccagc caccaccagc ccggcggcgg
     4621 gtgcgaaacc agcgttgaag caatcggtaa gaacatacca gccaggccaa ccgcgcgggg
     4681 tagtctgacg cgacgggcga agcgcagccc ggaccgctga tgttcgtttt gaggagttaa
     4741 aggaggttcc tccccatgcg ccgccgcttt tttgaaaaag ttttcatcat tcattatttt
     4801 tgggaacatt cagaaacaat tttcccaaat tatagagacg gggctaaagg cagaagtatg
     4861 aaatttcggg tgagccattt tcgtagctca cccgttagca ctatcaggta tcaggcggct
     4921 ttcttgaggc aggcgctcat aaacttatta cgatcatcac ctttcagaga ttgttgcgtt
     4981 gcttgattat tacattcgcg catcttttgc tgctgtggcg tcaaactttt ttcaccaggc
     5041 gcagacttgc tgttcttcag gcaatcactc atgtaggtct tacgagcatc ccctttcaac
     5101 gcctgcgccg tcgcctgctg attacaggag gtcatacgct gttgttgcgg ggttaaggtt
     5161 ctctcggcag cgccgacggt ggttaaaaaa accagaccga aaagcaaggt aaccagtaat
     5221 gttattttca tagcaccatc cctcttcatg ttttaaccat gagcatatgc gcccgtgatc
     5281 tgccattaag tctggttgct aacagcaaaa aaaccacccg acagcgaaaa ttcactgccg
     5341 ggcgcggttt tatttcagcc ccagggcggc tttcatggtg tagaagagat cggtctggtc
     5401 ggtcagtcca acgacattgg cggcatgtgg gccatacgca gcaatacgca gctgactacc
     5461 ggtatgttct tgtgaatcct cttcagagtt cccgtaactc atcaccatca ctgcgccatc
     5521 tttggtattt agcgcctggg tgaggcccgg cgctttggtg tccggcgcaa caatctggct
     5581 ggcgtgggcg tgatcagcgg tgactatgac cagcgtgttg ccatcctttt tagcgaattc
     5641 cagcgcacgt tgtacggctt catcgagatc gaccgtctcg ccaatttgcc cacaaggatt
     5701 cgcagcgtga tcctgtttat cgattgatgc accttcaact tgcaggaaaa aacctttctc
     5761 atttttactc aacaattcaa tggctttgtc ggtcatctgc gccagggtcg gtacgctgtc
     5821 attacgttgc ggattaggcg tacaggtaac tgcgggcttg tcgatattgc cgtggtacgt
     5881 tgctttcggt cctagccagc gcactggcat attgccgtca gcaaacagtc ctagcagggg
     5941 tttttgctgg ttcgcttccg tcaccgcatt tagcgaggca gcatcactca ccaactgata
     6001 accacgcgcc tgtgcctgtt cacgcagcgt ttttccctgc cattcgccga cggttgccgt
     6061 ttcagcaaag gtttttgcgc cgccgccaag cgtaacatcg gcacgagcgt taagcagctg
     6121 ttcggtaatc gatcctttcc cgcctttttc cagagcgtta cccggacatt tttcactggt
     6181 cgcgctcgga ccgtagcatt tgcgcgaggt cacatgcgcc accagcgcgg cgggcgtggc
     6241 atcctgcaac tctgcggtag aaacgttacc ggtcgccaga cctgcggctt ttgccatttc
     6301 cagaatcgtt gggtgatctt tttcgtgaat atcgacgccc agcgcaccgt tataggtttt
     6361 gacaccggtt gaccaggcgg ttgctgatgc agccgagtcg gtaacgtagt ccggtttgcc
     6421 ggttttttta ttcagcgcat agtgtgtgta ttgtccggta agcggtaagg catcgatacc
     6481 tttaaaaaag ccgcccgcac cttcggcata attgcgtgca gcagtaattt ccgagtcccc
     6541 catcccatcg ccaatcagca aaataatatt ttttgcaggt ttatcgctaa gagaatcacg
     6601 cagagcagcg gtctgatcac ccgttaagcg gcgagcaccg ccgggtgcag taatatcgcc
     6661 ctgagcagcc cggttttcca gaacaggcat ttctggtgtc cgggcttttg tcacagggga
     6721 aaacagtaac ggtaagagtg ccagtgcaat agtgctttgt ttcactttat tttctccatg
     6781 tacaaataca ttaaaaataa aaacaaagcg actataagtc tcggccgtga cagctttatg
     6841 acagctgttg aaaagattaa ctttttactg acgaggatgc ttcaataact tctttacata
     6901 attgcgcagc agctccgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag
     6961 cgccccctct acctgatcaa tcaacctttg ttggtcattt tgcgccatat tgcgaagcat
     7021 tgcagtgacg ataatctcca aagcttctac ctgcgcgcac agttctttcg actcttcttc
     7081 tttttgggca agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta
     7141 tccaaagtat ggagaagtta acattatgtt tcacaacttc atagtgtctg gaagtattat
     7201 ttttatgaca aaggaaatac tttgcgcaaa tattttttgt ttgattacca gcgaaacgtt
     7261 tctctta
//
LOCUS       DAYPHN010000199         7240 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000199 DAYPHN010000000
VERSION     DAYPHN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.199"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            98..439
                     /gene="yiaB"
                     /locus_tag="ACG0BW_004296"
     CDS             98..439
                     /gene="yiaB"
                     /locus_tag="ACG0BW_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFK7261181.1"
                     /translation="MKTSKTVAKLLFVVGALVYLLGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            484..921
                     /gene="yiaA"
                     /locus_tag="ACG0BW_004297"
     CDS             484..921
                     /gene="yiaA"
                     /locus_tag="ACG0BW_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFK7261182.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQITQEEYSE"
     gene            complement(963..1958)
                     /gene="wecH"
                     /locus_tag="ACG0BW_004298"
     CDS             complement(963..1958)
                     /gene="wecH"
                     /locus_tag="ACG0BW_004298"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFK7261183.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICATTLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2133..2441
                     /gene="ysaB"
                     /locus_tag="ACG0BW_004299"
     CDS             2133..2441
                     /gene="ysaB"
                     /locus_tag="ACG0BW_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFK7261184.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     GLCKDQAAKRYNTGAQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            2536..3447
                     /gene="glyQ"
                     /locus_tag="ACG0BW_004300"
     CDS             2536..3447
                     /gene="glyQ"
                     /locus_tag="ACG0BW_004300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFK7261185.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3457..5526
                     /gene="glyS"
                     /locus_tag="ACG0BW_004301"
     CDS             3457..5526
                     /gene="glyS"
                     /locus_tag="ACG0BW_004301"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFK7261186.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5848..6000
                     /gene="hokA"
                     /locus_tag="ACG0BW_004302"
     CDS             5848..6000
                     /gene="hokA"
                     /locus_tag="ACG0BW_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFK7261187.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(5977..6048)
                     /locus_tag="ACG0BW_004303"
     CDS             complement(5977..6048)
                     /locus_tag="ACG0BW_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261188.1"
                     /translation="MRNTVRKAGIISRRRRLLFQFAG"
     gene            complement(6188..6400)
                     /gene="cspA"
                     /locus_tag="ACG0BW_004304"
     CDS             complement(6188..6400)
                     /gene="cspA"
                     /locus_tag="ACG0BW_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFK7261189.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6681..6971)
                     /gene="yiaG"
                     /locus_tag="ACG0BW_004305"
     CDS             complement(6681..6971)
                     /gene="yiaG"
                     /locus_tag="ACG0BW_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFK7261190.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
BASE COUNT         1888 a         1706 c         1787 g         1859 t
ORIGIN      
        1 tatgaattta ttctgaaaat catttgttaa tggcattttt cagttttgtc tttcgttggt
       61 tactcgtaat gtatcgatgg tagatatgga gatcgttatg aaaacctcaa aaactgtggc
      121 aaaactatta tttgttgtcg gggcgctggt ttatctgctt gggctatgga tctcatgccc
      181 attgttaagt ggaaaaggct attttcttgg cgtgttaatg acagcaactt ttggtaacta
      241 tgcatatctt cgcgcagaaa aactcgggca actggataat ttttttaccc acatctgcca
      301 gttagtcgcg ttaatcacta tcggtctctt gtttatcggt gttttaaatg cacctatcaa
      361 tgcttatgaa atggtgatct accccatcgc cttttttgtc tgcttgtttg gtcaaatgcg
      421 tttgtttcgc tcggcatgag caacataaag ctcttacaca ttcaggaatg aaggaatact
      481 gtgatggaca acaaaatatc aacctattca ccggccttta gtattgtgtc atggatagct
      541 ctcgttggtg gtatcgttac ctatctgtta gggctatgga atgcagagat gcagttaaat
      601 gaaaaaggat attattttgc cgtactggta ttaggactgt tttctgctgc gtcttatcaa
      661 aagaccgttc gggacaagta tgaaggcata ccgaccactt ccatttatta tatgacctgc
      721 ctgactgtct ttattatctc tgttgcatta ctgatggtag gtctgtggaa tgcgacatta
      781 ttactcagcg agaaaggttt ttatggactg gctttcttct taagcttgtt tggtgcagta
      841 gcggtgcaga agaatattcg tgatgccgga ataaacccac caaaagaaac acagattacc
      901 caggaagaat acagcgaata attcacataa gcccggtcag tccaatgtga ccgggctttt
      961 atttaactca ctagtctgtt tctgtcgatt cgttgtacca gcatagaaag taacaaactc
     1021 gctgccaacg tcgcgcaaaa gatccaaata atatcgagta ttggccagtt tttaagctca
     1081 atcccccggg tgcgcagtgc atggataatc aaggcgtgga atccgtatat acccaacgaa
     1141 tggcgggaga ttaagccaag tccgcgaaca gtacgcgtat caagcgtgtt tttaaccaga
     1201 gtcaatagcg tggttgcgca gataaaaacc atcggcccac agtaaagata ccaggtatcg
     1261 gcaaaatttc cgcgccactg caattcatat aatgtcccgc gagagataat aaaaaccccc
     1321 gtcgcaaaca gtgcggcgca cacccacgac agtgctttat gctgtgtgtc catcatccct
     1381 atagcgcggc ccaacatgcc atacaggatg tagtaaaaag tatcgccatt gatatataag
     1441 ttaattggca gccattcaaa accgtcaatt ttctgcggca ctgtgtttgg gttagcgata
     1501 atgccaatca ccaccattag caccagcaac atttttccgc cgacgttctt cacctgaatc
     1561 agcggtgaaa ccagataaat caccgcaatc gcgaagaaaa accacaagtg gtaaaacact
     1621 ggcttttgca gcaggttttt cagcgctaac tccatattga tggaggtaaa cagcgcaatg
     1681 tagagcagtg cgattgcgct ataaaaaagc agacataagc cgatacgcaa gaaatggcgc
     1741 ggctgggcgc tgcgttcgcc aaaaaagaga tagccggaaa tcatgaaaaa tagcggcacg
     1801 ctgacacgag aggcagaatt cagaacattg gcgatatccc atgtgacggg gctaacacta
     1861 tgagcattgg tcacatacca ggtagtggtg tgaatcatca ccaccattaa acacgctatc
     1921 cctcgcaggt tatcaatcca gtaaattttg ggctgcatct gtgtctctgt atctggttaa
     1981 aaaaagtctg accgataaat cattcggttg gcgcactgga ataatctgag ttttatcact
     2041 acagcttata gaggcttaag gaaattcgta agatatcagc cactataccg atataaataa
     2101 taagactcac ctgcaaacca gacggtaatt taatgatgat gaactctttc tttccggcaa
     2161 tggcgcttat ggtgcttatg gcgctagtgg gttgttctac accgccaccc gtgcagaaag
     2221 cacaacgggt aaaggttgat cctctgcgtt cgttgaatat ggaagggcta tgcaaagatc
     2281 aggcggcaaa acgctataat acaggtgcgc aaaaaatcga cgtcaccgca tttgaacagt
     2341 tccaggggag ctacgaaatg cgcggttata ccttccgtaa agagcagttt gtctgttctt
     2401 ttgacgcaga tggccatttt ttgcatcttt ccatgcgtta agccctgctt tttcccgttt
     2461 cgtactgtat atcttccatc cagcgggtat actgatccct tcctttaaat ccacacgtat
     2521 ccagcacgaa ataatatgca aaagtttgat accaggacct tccagggctt gatcctgacc
     2581 ttacaggatt actgggctcg ccagggctgc accattgttc aaccattgga catggaagtc
     2641 ggcgcgggaa cctctcaccc aatgacctgt ctgcgcgcgc tggggccaga accgatggcg
     2701 gctgcttatg tccagccttc tcgtcgcccg accgatggtc gctacggcga aaaccccaac
     2761 cgtttacagc actactatca gttccaggtg gtcattaagc catcgccgga caatattcag
     2821 gagctgtacc ttggttctct gaaagagctg ggcatggacc cgaccatcca cgacatccgt
     2881 ttcgtggaag ataactggga aaacccgacg ctgggtgcct gggggctggg ctgggaagtg
     2941 tggctgaacg gcatggaagt gacgcagttc acttacttcc agcaggttgg tggtctggag
     3001 tgtaaaccgg ttaccggcga gatcacctac ggtctggaac gtctggcaat gtacattcag
     3061 ggcgtagaca gcgtttacga cctggtctgg agcgacggcc cgctgggtaa aaccacctac
     3121 ggcgacgtgt tccatcagaa cgaagtggag cagtccactt acaacttcga atacgcggat
     3181 gtggacttcc tgttcacctg ctttgagcag tacgagaaag aagcgcagca gctgctggcg
     3241 ctggaaaatc cgctgccgtt gccagcctac gaacgtattc tgaaagccgc ccacagcttc
     3301 aacctgctgg atgcgcgtaa agccatctcc gtcaccgagc gtcagcgcta tattctgcgc
     3361 attcgcaccc tgaccaaagc agtggcagaa gcatactacg cttcccgtga agccctcggc
     3421 ttcccgatgt gcaacaaaga taagtaagag gcggctatgt ctgagaaaac ttttctggtg
     3481 gaaatcggca ctgaagagct gccaccaaaa gcactgcgca gcctggctga gtcctttgct
     3541 gcgaacttta ctgcggagct ggataacgct ggcctcgcac acggcaccgt tcaatggttt
     3601 gctgctccgc gtcgtctggc gctgaaagta gctaacctgg cggaagcgca accggatcgt
     3661 gaaatcgaaa aacgcggccc ggcgattgcc caggcgttcg acgctgaagg caaaccgagc
     3721 aaagcggcag aaggctgggc gcgtggttgc ggtattaccg ttgaccaggc tgagcgtctg
     3781 actaccgata aaggtgaatg gctgctgtat cgcgcccacg tgaagggcga aagcaccgaa
     3841 gcactgctgc cgaatatggt tgcgacttcg ctggcgaaac tgccgatccc gaaactgatg
     3901 cgctggggtg caagcgacgt gcacttcgtg cgtccggtgc acaccgtgac cctgctgctg
     3961 ggcgacaaag ttattccggc taccattctg ggcattcagt ccgatcgcgt gattcgcggc
     4021 caccgcttta tgggcgagcc ggaattcact atcgacaatg ccgatcaata cccggaaatt
     4081 ctgcgtgagc gcgggaaagt catcgccgat tacgaagaac gtaaggcgaa gattaaagcc
     4141 gatgccgaag aagcggcgcg taagattggc ggtaacgctg acttaagcga aagcctgttg
     4201 gaagaagtcg cttcgctggt ggaatggccg gttgtgctga ccgcaaaatt cgaagagaaa
     4261 ttcctcgcgg tgccgtctga agcgctggtt tacaccatga aaggtgacca gaagtacttc
     4321 ccggtgtatg cgaacgacgg caaactgctg ccgaacttta tcttcgttgc caacatcgaa
     4381 tcgaaagatc cgcagcaaat tatctctggt aacgagaaag tcgttcgtcc acgtctggcg
     4441 gatgccgagt tcttcttcaa caccgaccgt aaaaaacgtc tggaagataa tctgccgcgt
     4501 ctgcaaaccg tgctgttcca gcaacagctt ggcacactgc gtgacaaaac tgaccgcatc
     4561 caggcgctgg ctggctggat tgctgaacag attggcgctg acgttaacca cgcaacccgt
     4621 gcgggcctgc tgtccaagtg cgacctgatg accaacatgg tcttcgagtt caccgacacc
     4681 cagggcgtta tggggatgca ctacgcgcgt cacgatggcg aagcggaaga tgttgcagtg
     4741 gcgctgaacg agcagtatca gccgcgcttt gccggtgatg acctgccgtc taacccggta
     4801 gcctgtgcgc tggcgattgc tgacaagatg gatactctgg cgggtatctt cggtatcggt
     4861 cagcatccga aaggcgacaa agacccgttt gcgctgcgtc gtgccgcact tggcgtgctg
     4921 cgtattatcg ttgagaagaa cctcaatctt gacctgcaaa ccctgaccga agaagcggtg
     4981 cgtttgtatg gcgataagct gacaaatgcc aacgtggttg atgatgttat cgactttatg
     5041 ctcggtcgct tccgcgcctg gtatcaggac gaaggttaca ctgttgacac catccaggcg
     5101 gtactggcgc gtcgtccgac tcgtccggct gatttcgatg cccgaatgaa agcggtatcg
     5161 cacttccgta ccctggatgc agctgcagca ctggcggcgg cgaacaagcg tgtatctaac
     5221 attctggcga aatctgacga agtgctgagc gaccgcgtga atgcctctac tctgaaagag
     5281 ccggaagaaa ttaaactggc gatgcaggtt gtggtgctac gtgacaagct ggagccgtac
     5341 tttgcggaag gtcgttacca ggatgcgctg gtcgaactgg cagagctgcg tgaaccggtt
     5401 gatgccttct tcgataaagt gatggtcatg gttgatgaca aagaattgcg tatcaaccgt
     5461 ttgaccatgc tggagaaact gcgcgaactg ttcctgcgcg ttgcggatat ttcgctgtta
     5521 cagtaataac gccattatta aaaagcctgc catctggcag gcttttttat cgctaaataa
     5581 tagagcaacc tttaataatc ttctgctgaa taaagattgt ctcatatatt aattttatga
     5641 gattttttta ggattatatc aaggagaaga aacgaactta ttaagctaga atagctgcgg
     5701 gtgcttgaga ctgtttgtct caggtattca ccgaaaggca gacagagaaa agccccacct
     5761 gactataaat caaagtgagg cccctctatg catagacacc ataaggttag cctcttactc
     5821 gttggaaatc aacacagggg gctgggaatg ccgcagaaat atagattact ttctttaata
     5881 gtgatttgtt tcacgctttt atttttcacc tggatgataa gagattcact gtgtgaattg
     5941 catattaaac aggagagtta tgagctggcg gcgtttttag cctgcaaatt gaaagagtaa
     6001 gcgtcttcgg cgggaaatta ttcccgcctt tcttacggtg ttgcgcattc tcattgcacc
     6061 caaatttatt cttcacaaaa ataacactag attctattac gcgatcgatt atttatttcc
     6121 tgaaaaacaa ataaaaaaat ccccgccaaa tggcagggat cttagattct gtgcttttaa
     6181 gcagagatta caggctggtt acgttgccag ctgccgggcc tttagcgccg ctttcgatgg
     6241 tgaaggacac tttctgacct tcgtccagag atttgtaacc atcgttctgg atagcagaga
     6301 agtgtacgaa cacgtcttta gagccatcgt caggagtgat gaagccgaag cctttgtcag
     6361 cgttgaacca ttttacgata ccagtcattt taccggacat agtgtattac ctttaataat
     6421 taagtgtgcc tttcggcgat atggcgtgct ttacagattt tgaagcgtta aaggaatgtg
     6481 cactacgagg ggtatcaacg ataactcttg aagggacttg ccttactaca ctgctttaat
     6541 ggtctgtacg tcaaaccgtt gatgtgcatt aagccacgca ttggcgggtg atgcaacaat
     6601 tatttttcat atttatgatt aatcggttgg aaaacggtcc tgtcatcagg accgtaaaca
     6661 gcaataaagt ggataaaagt ctattccatc aactgcttac ttaatgccgg gttggcttga
     6721 atcaaacgca tcaattttag ttcggcactt gaaggcttca cgcgtctgga ttcccattcc
     6781 tttaccatgg cgactgatac gcccaaaacc cgggcgaaat catcgatttt taaccctgtc
     6841 ccttttcgta actgctcaat ttcggtacag gacgttgttc tgtgcgtcag ggtaattttc
     6901 tgcgtttcat ctttaaaaac aatctgttcc aggctgctca acagctcatg cattggatct
     6961 ttatattcca ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc atcgaagctc
     7021 attaagaata gtcgggaaaa acagacacag cggggtgtag tcagggcatg ctctggctat
     7081 gattcgattc agccagtcat ttagcgtgac ttatagccac ctaacgatct gataaatgcc
     7141 gatatatctc ttaccactcc cggtttgtaa agatttcgaa aatctaacgt tctgagaagg
     7201 cgttaatctc tggcaacaaa aaaagcaacg gcactatcat
//
LOCUS       DAYPHN010000200         7147 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000200 DAYPHN010000000
VERSION     DAYPHN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.200"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..457
                     /locus_tag="ACG0BW_004306"
     CDS             <1..457
                     /locus_tag="ACG0BW_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFK7261191.1"
                     /translation="GKLAAKFRASGQPVFLVRVGWSADYAEALKQPVDVPSPAKVLPE
                     NWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTA
                     RNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            454..1272
                     /locus_tag="ACG0BW_004307"
     CDS             454..1272
                     /locus_tag="ACG0BW_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFK7261192.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRDLPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1325..1720
                     /locus_tag="ACG0BW_004308"
     CDS             1325..1720
                     /locus_tag="ACG0BW_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFK7261193.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1761..2504
                     /gene="cmoA"
                     /locus_tag="ACG0BW_004309"
     CDS             1761..2504
                     /gene="cmoA"
                     /locus_tag="ACG0BW_004309"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFK7261194.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRNIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HQAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2501..3472
                     /gene="cmoB"
                     /locus_tag="ACG0BW_004310"
     CDS             2501..3472
                     /gene="cmoB"
                     /locus_tag="ACG0BW_004310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFK7261195.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(3507..5936)
                     /gene="torZ"
                     /locus_tag="ACG0BW_004311"
     CDS             complement(3507..5936)
                     /gene="torZ"
                     /locus_tag="ACG0BW_004311"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFK7261196.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEISGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTSNAPK
                     LTAFDQPAVQA"
     gene            complement(5961..7061)
                     /locus_tag="ACG0BW_004312"
     CDS             complement(5961..7061)
                     /locus_tag="ACG0BW_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFK7261197.1"
                     /translation="MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHTKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASIQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDISEKQ"
BASE COUNT         1613 a         1873 c         1743 g         1918 t
ORIGIN      
        1 cgggaagctg gcggcgaaat ttcgcgcgag cggtcagccc gtgtttctgg tgcgcgttgg
       61 ctggtctgcc gattatgccg aagcattaaa acagccggtt gatgtcccct cccccgctaa
      121 agtgttaccc gaaaactggt ggcaacatcc tgctgcatta ggtgcaaccg acagcgatat
      181 cgaaatcatc aaacgtcaat ggggtgcgtt ttacggtacg gatctggagt tgcaattacg
      241 ccgccggggt atcgatacca ttgttttatg tgggatctcg accaatatcg gtgttgaatc
      301 caccgcccgc aatgcctggg aactcggttt taatctggtg attgccgaag atgcctgtag
      361 cgccgccagc gccgagcagc acaataacag catcaaccat atctacccac gcatcgcccg
      421 tgtgcgcagt gtggaagaga tcctcaacgc attatgattt acatcggtct gccgcaatgg
      481 tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc ccgccacttt
      541 aactgtgtgg agggcaacac cacgctatac gccctgccca aacccgaggt tgtcctgcgc
      601 tggcgtgagc agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg
      661 catcaggcag cattacggca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca
      721 ccgttagcgc cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt tggcccacgg
      781 gatctgcctg cgctttggca ttttctcgat tctcttcccg gcgaatttaa ttatggtgtg
      841 gaagtccgcc atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt
      901 ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcagcacgt
      961 ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct
     1021 gtactgacgg cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac
     1081 cgggaattat ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct
     1141 tatctttttt tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg
     1201 gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct
     1261 tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt
     1321 ttgtatggta agcgcgctgt atgccgtttt aagtgcgttg ttattaatga agttctcttt
     1381 tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga
     1441 actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt
     1501 gctgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat
     1561 cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgttccgctg
     1621 gcgacgttcc ggcatgagcg ccacatggtg tgcgctgttg ctgatggtgc tggcgaatct
     1681 ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct
     1741 ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca
     1801 gactgggtga ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc
     1861 gttccgttcc cggttattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg
     1921 ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg
     1981 tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgacaac tctccggcga
     2041 tgattgaacg ctgccgtcgt catattgacg cctataaagc ccctacgcca gtagacgtta
     2101 ttgaaggtga tattcgcaat atcgccattg aaaacgcatc gatggtggtg ctgaatttta
     2161 ccctgcaatt cctggaacct tccgagcgtc aggcgttact ggataaaatt tatcaagggc
     2221 tgaacccggg cggtgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg
     2281 gtgaattgct gttcaacatg caccacgact ttaaacgtgc taacggttac agcgaactgg
     2341 agatcagcca gaagcgcagc atgctggaaa acgtgatgct aaccgattct gtggaaaccc
     2401 ataaagcacg cctgcatcaa gctggttttg agcatagcga gctgtggttc cagtgcttta
     2461 actttggttc cctggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt
     2521 ttattctctg attgccaaaa atcatctttc acactggctg gaaacgctgc ccgcgcagat
     2581 tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acgcggtgga
     2641 atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag
     2701 cgaagagcca ctgagcgccg ggcaaattaa acgcattgaa acgctgatgc gcaacctgat
     2761 gccgtggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc
     2821 cgactggaaa tgggatcgcg ttctgcccca tctttctgat ttaaccgggc gcaccattct
     2881 tgatgtcggc tgtggcagcg gttatcacat gtggcgcatg attggcgcag gcgcacatct
     2941 ggcggtgggg atcgacccta cgcaactgtt tctgtgccag tttgaagcag tgcgtaaatt
     3001 gttaggtaac gatcaacgtg cgcatctgct gccgttaggc attgaacaac ttccggcact
     3061 gaaagccttt gataccgtct tttcgatggg cgtgctttat catcgtcgtt caccgctgga
     3121 gcatctctgg cagttgaaag atcaactggt caatgaaggt gaactggtgc ttgaaacgct
     3181 ggttattgat ggtgatgaga acacggtgct ggtgccgggc gatcgttacg cgcaaatgcg
     3241 taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt
     3301 tgttgatatt cgcattgcgg atgtgagcgt taccactaca gaagaacagc gacgcaccga
     3361 atggatggtt actgaatcgc tggccgattt cctcgaccca catgatccgg gtaaaacggt
     3421 ggaaggttat cctgcgccta aacgcgcggt gctgattgcg cgtaagccgt aaagattagc
     3481 gcccgtttcc gggcgctttt cacgtcttat gcctgaacag ctggctgatc aaacgccgtt
     3541 aacttcggcg cattgctcgt atatttttcg atatacacca gcgcagagtt accggcacag
     3601 gcatttgcca gctgagagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     3661 cacaagccat tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     3721 atcccgtcag tcaccaccgc gcctgtcaga atctgaccac gtttgttcca gacacgcacc
     3781 agatcgccat tcgcaatacc aaagcgagca gcatcttcgg tgtgaatagt gatcggttca
     3841 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacga
     3901 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     3961 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     4021 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     4081 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     4141 ttattttgct gccagaaggc attaaacatc ggcatagtca cgcgttgcgc acgggcacct
     4201 ttctgagcgg catcatagaa aaatttcagc cacgccatct catctttacc ttcggtatag
     4261 atctcttttc cgccaggttt cagtaattca gcaagatcgg caaacacgtc aaaatcgtta
     4321 cgcgcttcaa attgcgcagc gacagcctgc ttcatcggta caatatgctg gttgctgtaa
     4381 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     4441 gcatgtttag cagctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     4501 catgctttaa tcagacgatt agtatcctgg tgatgggtaa agttcccgcc acccgcccac
     4561 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     4621 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc gccatcatca
     4681 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     4741 cgcgtcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     4801 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     4861 tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc catcagttca
     4921 gcgagtttga ctatctgcgc ttccggtacg ccagaaattt ccgccgccca ggccgcgctc
     4981 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata tccggtagtg
     5041 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     5101 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
     5161 aattcgatgg tttcggagcg gataggatca atggcaatca cgggtttgcc agattttttc
     5221 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
     5281 ttcagcgggt tcatccccca cagcaccaca acctggctgt tttccagaat cagcggccag
     5341 gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgcc
     5401 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgcaaggtt catataacgt
     5461 tgcagtaagg tttgcgcttt atgcaacacg ccagaagatc gccagccgta agatccggca
     5521 aaaatggccg atggtccgtt agctttacga atacgatcgt gttgctcatg aatcagtttt
     5581 aatgcttgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt tgccggttgc
     5641 agtggattat cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
     5701 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
     5761 gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg tcccgcggtg
     5821 aggattttac cgcccttctc ttctgcccat gcgggtagcg gtgcagccga tgtcaccacc
     5881 agcgccccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgtcataact
     5941 tcctccctga tcaacgagga tcactgtttt tcgctaatat ctttggcgtt gtactggaag
     6001 taccgcgtca aaatgtccag ttcattttcg ctcatgctgg ttcgcgcccc cattcctttg
     6061 gcaatggacg gccaggcatt gacggtgtaa tggtcagcgg caataggcgc atggcaacca
     6121 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
     6181 gcgggatcgg taagcacacc ctgtatagac gcctgacgcc attgattgcc gtactcgtca
     6241 gcctgccatt ccccgtttac cgtcagcgcc ttgatacctt cttcacttaa tgtggctagc
     6301 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
     6361 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
     6421 ttcacagtgg ccagttcgcc tatgcgggaa ggtttgaaag gataaatatg tgcgccattg
     6481 gtaactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
     6541 tctggtggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gtccttttgc
     6601 gctttggtat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
     6661 gaatggcaac tacgacacgt tgcagaatca gttgctttta attctttcca tactgtttcg
     6721 gccatttcct gacgatgcgc ttcgaattta tcgtcactgt ctattttgcc gctaacaaat
     6781 tcatgataaa tatctttaga agcttttaat ttagcaaata aataatccat ccctgacttt
     6841 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
     6901 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
     6961 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
     7021 atcagcaaaa acattaaccc aatgcgtttt ttccctcgca tagattcctc taattaattc
     7081 cagaacaccg aaatcattgt ataattaaat atacaacgaa ttgaccgctt gcataattcg
     7141 gcacaac
//
LOCUS       DAYPHN010000201         7139 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000201 DAYPHN010000000
VERSION     DAYPHN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.201"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1339
                     /gene="gdhA"
                     /locus_tag="ACG0BW_004313"
     CDS             <1..1339
                     /gene="gdhA"
                     /locus_tag="ACG0BW_004313"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFK7261198.1"
                     /translation="QTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYR
                     QMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLS
                     ILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADT
                     DVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTE
                     AMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEK
                     LARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLI
                     ANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWK
                     AEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(1416..3371)
                     /gene="topB"
                     /locus_tag="ACG0BW_004314"
     CDS             complement(1416..3371)
                     /gene="topB"
                     /locus_tag="ACG0BW_004314"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFK7261199.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(3376..4419)
                     /gene="selD"
                     /locus_tag="ACG0BW_004315"
     CDS             complement(3376..4419)
                     /gene="selD"
                     /locus_tag="ACG0BW_004315"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFK7261200.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(4536..5087)
                     /locus_tag="ACG0BW_004316"
     CDS             complement(4536..5087)
                     /locus_tag="ACG0BW_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFK7261201.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPYRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            5248..7104
                     /gene="sppA"
                     /locus_tag="ACG0BW_004317"
     CDS             5248..7104
                     /gene="sppA"
                     /locus_tag="ACG0BW_004317"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFK7261202.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RSAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
BASE COUNT         1617 a         1928 c         1881 g         1713 t
ORIGIN      
        1 tcagacatat tctctggagt cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac
       61 cgagttcgcg caagccgttc gtgaagtaat gaccacactt tggccttttc ttgaacaaaa
      121 tccgaaatat cgccagatgt cattactgga gcgtctggtt gaaccggagc gcgtgatcca
      181 gtttcgcgtg gtatgggttg atgatcgcaa ccaggtacag gtcaaccgtg catggcgtgt
      241 gcagttcagc tctgccatcg gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa
      301 cctttccatt ctcaaattcc tcggcttcga acaaaccttc aaaaatgccc tgactactct
      361 gccgatgggc ggtggtaaag gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga
      421 agtgatgcgt ttttgccagg cgctgatgac tgaactgtat cgccacctgg gcgcggatac
      481 cgacgttccg gcaggtgata tcggggttgg tggtcgtgaa gtcggcttta tggcggggat
      541 gatgaaaaag ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg
      601 cggcagtctt attcgcccgg aagctaccgg ctacggtctg gtttatttca cagaagcaat
      661 gctaaaacgc cacggtatgg gttttgaagg aatgcgcgtt tccgtttctg gctccggcaa
      721 cgtcgcccag tacgctatcg aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc
      781 agactccagt ggcactgtag ttgatgaaag cggattcacg aaagagaaac tggcacgtct
      841 tatcgaaatc aaagccagcc gcgatggccg agtggcagat tacgccaaag aatttggtct
      901 ggtctatctc gaaggccaac agccatggtc tgtaccggtt gatatcgccc tgccttgcgc
      961 cacccagaat gaactggatg tagacgccgc gcatcagctt atcgccaatg gcgttaaagc
     1021 cgtcgccgaa ggggcaaata tgccgaccac catcgaagcg actgaactgt tccagcaggc
     1081 aggcgtactg tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa catcgggcct
     1141 ggaaatggcg caaaacgctg cgcgcctggg gtggaaagcc gagaaagttg atgcgcgttt
     1201 gcatcacatc atgctggata tccaccatgc ctgtgttgag catggtggtg aaggtgagca
     1261 aaccaactac gtgcagggtg cgaacattgc cggttttgtg aaggttgcgg atgcgatgtt
     1321 aagccagggc gtgatttaag acaattgtgc cggatacagc gcaaacgcct tatccggcca
     1381 taaaaatctt cagtatcaga tggttgaaag aacctttata ttatcactcc attgcccgcc
     1441 tcttctgatg gcgggctttt tttcggcgct ctcttgcgtg gcgaatcctt tttcttccct
     1501 tctccaccgc cgggagccat tattccccga aactgcctta ccgacgtgcg ctttgcctga
     1561 tcaatcaact ggtacaatgt ccccaccagc ggctgcataa agtcctgata gcgacactgc
     1621 ttttcactga tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg tcgcgtcgcc
     1681 atttctggca gcgaatggaa tagcgctttt ccggcgtcgg tggagtggat atagcgccct
     1741 tttttggtca ggaagcctcg cttgaacagc agttcaataa tcccggcacg ggttgcttct
     1801 gtccccaggc catcggtcgc acgaaggatc tttttcagat ctttgtcctg cacaaagcgc
     1861 gcgattccgg tcatcgccga aagcagtgtt gcatcggtaa aatgacgcgg cggctgggtt
     1921 tgccgctcta ccacttcacc tttttcacac agcaactcat cgcctttcgc caccacaggc
     1981 aatggcgtac cgtcgttttc ttcatcgcgc tctttgctgc ctaacagcgt gcgccagcct
     2041 gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt tggcaatatc cagttcgata
     2101 acacacttgc ggaacaccgc atccgggcag aactgcatca agtactgacg ggcaatcagg
     2161 ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag aactccgtgc ggtcgggatg
     2221 atggcgtggt gtgcatcgac ctttttgtca tcccagcagc ggttgcgtat atctggatcg
     2281 accactggct gcggcaacag atctggtgca tgaacgctga tggcattcat caccgcgtgg
     2341 cgtccggcaa aatgttcttc tggcaaatag cggcaatcgg aacgcggata agtgattagc
     2401 ttgtgcgttt cgtacaactt ctggcaaata tcaagcacgt tctgcgcgct cagaccaaag
     2461 cgttttgccg cttcaatctg caacgccgaa agagaaaacg gtagcggtgc ggattctgat
     2521 tcccgtttat cgttatagct ggtgacaata gccggttggc cgctgatgcg gttgaccaca
     2581 tgctccgcca gcggacgatg taacaagcgc ccttcttcat cctgataagg ttcacacgct
     2641 tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat atgcgctttg
     2701 acttcaaaga aatctttcgc cacgaagttt tcaatctctt cgtcgcggcg cactaccagc
     2761 ccgagcacgg gcgtctgcac gcgtcccacg gaaagtacgc cctgataacc ggcattgcga
     2821 ccgagaatag tatacgcacg ggtcatattg atgccgtaca gccagtcggc tcgggctcgc
     2881 gccagcgcag aaacgcacag cggtacaaac tcactgttgg aacgaagacg gtcgatcgcc
     2941 cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac gctgcacctg ctggcgcttt
     3001 tccggtgcca gttgcagata gtccagcact tcatccacca gcaattgccc ttcacgatcc
     3061 gggtccccgg cgtgaacgat ttcgctggct tcatgcagga agcgtttgat gacgttaagt
     3121 tgtttggtca cggagggtcg gggctgtaat tgccactttt ccgggacaat cggcaaatcc
     3181 gcaagattcc agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg ctcaagcagg
     3241 tgaccgatac accaggtcac tacctgacca ttaccgcact cgataaagcc atcgcctttc
     3301 cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca gactcggttt ttcggcaata
     3361 aacaaccgca ttgaattaac ggatctcaac catggcacga ccgccgcgcg ctggcaccag
     3421 ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct gtagctttaa cctcattttc
     3481 tgcttccggc atgaccgcca gcagcaaacc gccagaagtt tgcggatcgc acagcaaatc
     3541 gcgcacttca cgcggcattt cacccatcag atgaccgtag ctggcaaagt tacgttcggt
     3601 gccgccaggc actgcgccca acttaatgta ctcttcaacc cccggcagtt tcgggatcgc
     3661 ttcatagtcg acgcgcgcct gcacaccagc accctgacac atttcgctta aatgccccag
     3721 cagaccaaag cccgtaacgt cggtcatcgc ttttacgcct tcgatgttgg caaaggacgc
     3781 gcctgcgatg ttcatccggc acatcacttc cgtcgccagt ccctgatgtt ctggtttcag
     3841 caatgatttt ttctcagccg tagtaagaac gccgatcccc agcggtttcg tcaggaacag
     3901 tttacatccg gcttgtgcgg tactgttttt cttcacccgc tcggtcggta cgatccccgt
     3961 taccgccaga ccaaaaatcg gctccggcgc atcgatggag tgaccgccag ccagcgcaat
     4021 acccgcctga cggcatgcat agcgtccacc ttcggttact tcgcgggcaa tttccgggga
     4081 aagtttgtta atcggccagc cgaggatcgc aatcgccata atcggtttgc cccccatcgc
     4141 gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca aaatcgaaag gattatcaac
     4201 gatcggcata aagaagtcgg tggtactgat aacgctggtg ccattgccca gatcgtacac
     4261 cgccgcatcg tcgcgggttt cattacccac aagcaaattc ggatcaacaa acttcgcctg
     4321 ctcactgtgc aggatggttt ccaacacttt tggggaaatt ttacagccgc aaccagctcc
     4381 gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg gacatctcct gtcaatgcaa
     4441 tccgggtatg gtaaccctca ttccatgaag tgataagtga gagtgtctga attcctgcgc
     4501 ctttgctcac aatccagaca gtttcgcgac aattatcaga aataagttac aaacggcgtc
     4561 gggtccggga cgtttatcga cgtagatgct ttcagctgcg gcgtaccaag gtagagaaaa
     4621 ccgacaattt tatcctgctc acggcaaccg aatgcttcac gcactaccgg actttcagtt
     4681 aatgcgccac tgcgccagat gccgccaaac ccctgggcaa ctgctgccat ttgcatcgcc
     4741 atgaccgcgc atcccgcaga catttcctgt tcccagcgcg ggactttatg attttcttcg
     4801 cattttgcca ccaccgtgat gatgagcggt gcgcggtacg gcgcattacg ggctttgtcg
     4861 atggctttgt catcactacc ggcagcaatc gccccctgct ccagaacggc gctgaaacgc
     4921 tcgcgtcctt ccccttcaat cacaaaaaaa tgccacggtt gcatggactt atggtccggc
     4981 gcacgcatac ccgctcgcag tatgttttgc agttgttcac ccgttggcgc aggttcagcc
     5041 aggcgggagg cgctacggcg attgatcaat agttcgagtg catccatttg attaactcct
     5101 gtcatgatat ttattcacaa aattaacacg agagtggatt ttgttacagc acagtccgca
     5161 attcctgctg acaagtgccg gttgggtcat tacgataacc acatctattg cgcctgtgac
     5221 aggtgtgacc ttaagttggg agaatacatg cgaacccttt ggcgatttat tgccggattt
     5281 tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa tggtacttaa cctgttcttt
     5341 atttttctcg tactggttgg tgtggggatc tggatgcagg tcagtggtgg tgattcgaaa
     5401 gaaacggcca gtcgtggcgc gctgctgctg gacatttctg gtgtgatcgt cgataaaccc
     5461 gacagttctc agcggtttag taaattaagc cgccaactgc ttggtgccag ttccgatcgt
     5521 ctgcaggaaa actcactgtt tgatatcgtc aataccattc gccaggcgaa ggatgaccgc
     5581 aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag gcggcgacca accgtctatg
     5641 cagtacatcg gcaaagccct gaaagagttt cgtgacagcg ggaaaccggt ttatgccgtt
     5701 ggcgagaact acagccaggg gcaatattat ctcgccagtt tcgccaataa aatttggctg
     5761 tctccgcaag gcgtggtgga tctgcacggt tttgctacca acggtctgta ctacaaatcg
     5821 ttgctggata agctgaaagt ttccacccat gtgttccgcg tgggtacgta taaatctgcc
     5881 gttgaaccgt ttattcgtga tgatatgtca ccggcagccc gcgaagctga cagccgctgg
     5941 attggtgagc tgtggcaaaa ctatctgaat actgttgccg ctaaccggca gatccctgct
     6001 cagcaggtat tccctggcgc gcaagggttg cttgagggtt taaccaaaac cggtggcgat
     6061 accgcgaaat atgcactgga aaacaagctg gtcgatgcac tggcatcgag tgcggaaatc
     6121 gaaaaagctc tgaccaaaga gttcggctgg agtaagactg ataaaaatta tcgcgccatc
     6181 agttattacg attacgcatt gaaaacgccg gcagataccg gtgacagcat cggtgtcgtc
     6241 ttcgcgaatg gcgcaattat ggatggcgag gaaactcagg gaaatgttgg cggtgatacc
     6301 actgcggcac aaatccgcga cgctcgcctt gacccgaaag tgaaagcgat tgttctgcgt
     6361 gttaatagcc caggtggcag cgttaccgcg tccgaagtga ttcgcgctga actggctgcc
     6421 gccagatcag cgggtaaacc tgtggttgtg tcgatgggcg gtatggcggc atctggtggt
     6481 tactggattt ccacgccagc taattacatt gtggctaacc ccagcaccct gaccggttct
     6541 atcggtatct tcggcgtgat caccaccgta gaaaatagtc tggattcgat tggtgttcat
     6601 actgatggtg tatcaacttc accgttggcg gatgtttcta tcaccagggc actgccgccg
     6661 gaagcgcagc agatgatgca gttaagcatt gagaatggct ataaacgctt tatcacgctg
     6721 gttgctgatg cgcgtcattc gacgccggag cagattgata aaatcgccca gggccacgtc
     6781 tggaccggtc aggatgcaaa agctaacggg ctggtcgata gtctcgggga tttcgatgat
     6841 gcggtcgcca aagcagcaga gctggcaaaa gtgaaacagt ggcatctgga atactacgtt
     6901 gatgaaccga ccttcttcga caaagtgatg gacaacatgt ctggttctgt ccgggcaatg
     6961 ttgccagatg cgttccaggc catgttacct gcaccgctgg cctcggtagc ctctaccgtt
     7021 aaaagtgaaa gcgacaagct ggcagcgttt aacgacccac aaaaccgtta tgcgttttgc
     7081 ctgacctgcg ccaacgtgcg ttaagtcttg tactaagtgg ccgacagatc gtcggccac
//
LOCUS       DAYPHN010000202         7108 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000202 DAYPHN010000000
VERSION     DAYPHN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.202"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(97..1488)
                     /gene="uhpT"
                     /locus_tag="ACG0BW_004318"
     CDS             complement(97..1488)
                     /gene="uhpT"
                     /locus_tag="ACG0BW_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFK7261203.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(1626..2945)
                     /gene="uhpC"
                     /locus_tag="ACG0BW_004319"
     CDS             complement(1626..2945)
                     /gene="uhpC"
                     /locus_tag="ACG0BW_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFK7261204.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLDVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(2955..4457)
                     /gene="uhpB"
                     /locus_tag="ACG0BW_004320"
     CDS             complement(2955..4457)
                     /gene="uhpB"
                     /locus_tag="ACG0BW_004320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFK7261205.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPAELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(4457..5047)
                     /gene="uhpA"
                     /locus_tag="ACG0BW_004321"
     CDS             complement(4457..5047)
                     /gene="uhpA"
                     /locus_tag="ACG0BW_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFK7261206.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELISAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(5209..6678)
                     /locus_tag="ACG0BW_004322"
     CDS             complement(5209..6678)
                     /locus_tag="ACG0BW_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261207.1"
                     /translation="MVSVTSYTYPTETENDNSQFQSQTQSQGLSQSLRDSIEGNIAAF
                     DAMEQSGLLDNCRTLSKDATFREVFRNILLYILDSFHIYRHDISGKLVSYFHVTPESA
                     NANYKTWIATTGMDSYIKIEQTGSDKFKVTDVYRELNGKSLEKKSRDRDFSGRNVKEF
                     IGLLSEHDVLEKYQLSKVTVAANALKLEVVNNNFKNELSSGVTDAEHQLKKALYEQTQ
                     STPDKSIQYELIPDKLIPDDCSYKSDIKKLDVREGEFSALLADASRHVGEASSFIACC
                     GVKINSTIHELLRSKTMALSGHGGVRKYKDFEKLFSEIVFTPDECNIVRNSLQKDGVE
                     KKTRENIISCLSSISGIGNLYYACSQAFPVALDRGDINAIQASNSISAGFNSNPGFCA
                     LMGKTNFLFADFSQHTFTQTHYIVKAEINEAGNDFNIANFIYRTYGEDYIHNDSVDDS
                     LNERSAEDYRFLDDSLLPENDFRNRRRNFSGNIAIATYK"
     gene            complement(6954..>7108)
                     /locus_tag="ACG0BW_004323"
     CDS             complement(6954..>7108)
                     /locus_tag="ACG0BW_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261208.1"
                     /translation="KMYSMAVNNVGNEVKLQCSRYFTTGRVDEQNSKIDKVTFTFFGA
                     ATTADL"
BASE COUNT         1950 a         1916 c         1619 g         1623 t
ORIGIN      
        1 aaagctgaaa gctttccgga acccccagcc tagctggggg ttttctgtgc acaaaaaaag
       61 cccggcgtca tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat
      121 ttttttctcg cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat
      181 acaaccaatc gcggcgatat ccagcgcagc gaaggtgcct gcccagccgg taaggccgaa
      241 tactggcgtc ccatcggcaa tcattcccag acctaacttg gcaaagctgt cgccaatcag
      301 gtaagcaaag gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc
      361 aacagcagcc acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag
      421 agaagccagg tagatatatt cgttgctggc gtgttgatac acaccgagcg tggcgataat
      481 cagcgccagc gcgatacagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca
      541 gccccacagc agcgtaccga ccagcgcgcc agcttcaaac agcgtaaagc cctgaatcgc
      601 caccgcttta gagagtttca gttcctggaa cgcgtacaca gttgaccact ggtcgatacc
      661 aatacgtacc acatagagga aaatgttggc gaagcacagc agccagatca ctttgttttt
      721 cagcacatac tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc
      781 ctcttcgctg atctcctcgc cgaacagttc ttcagctttg ccgaggccat aagattccgg
      841 ggagtcgctg ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa
      901 gataaacatg ccgatgacat ggccatcgaa caggtaattt gcgccgaaca gcgctacacc
      961 tgccgcgcct gcaccgccaa ggttgtgaga aatgttccag aaaccgagga aggtcccgcg
     1021 cttacgacgc ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtgctctg
     1081 gaaaaagccg cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc
     1141 catactggcg ctgaagccca gcatacaaat ggcagacagg atcagcatga acggcaggaa
     1201 ttgtttggtg tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt
     1261 gatggagaag cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgaaat
     1321 catatcgttc tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat
     1381 aaagaccacc aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag
     1441 cggaaggtcc agggtcggct tgcggacctg gtttaagaaa gccagcatgg gttactcctg
     1501 aaatgaatac ctgcgtgagg catgcattgt aaaaatcagc agcaaatcag gcctgagata
     1561 gcgtccaggc aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag
     1621 atgcatcacg cttcgcgcgg tgcctgggcg ttcaaaaagg gcagtaacag cagtgcggaa
     1681 atcccggcgg cgatagagat aaccacaaaa aatccgctcc agtgccaggt atcgagtact
     1741 ttcgccagtg gccaaccagc aagcgacgcc cccagataag caaacaagcc gacaaacccc
     1801 gtcgccgccc ctgccgcctc tttgtgggaa cactctgccg ccgccatgcc gattaacatc
     1861 tgcgggccaa agacaaaaaa accaatagtg aagaagcagg ttgcctgcat cacgtagctg
     1921 gcaaatggca tcagccacag cgagccgact gaaagcaaaa ttccggcggc gaaaatcaaa
     1981 ttcatcggcc cacggttgcc gttaaacaat ttgtccgagc cccaaccggc taccagcgca
     2041 ccgataaatc cgcccagttc aaacatcgtc actgccgtat tcgccgtgac cagatcgacg
     2101 tccagcgtct cggacatata caaattgccc cagtcgttga ttgccgcccg gaccacatag
     2161 accagcacat agcaaaacga aagcagccag atatacggat tcagtaacac atacttggtg
     2221 aggatctctt tacgcgtcag ccctgcccct tcttgttgtt gagcaatctc cagcgcgtcg
     2281 tgccgccatt caccgaccgc cggtaaacct aacgcctgcg ggcgatcgcg cagccgccag
     2341 cagagaaaaa tccccacgac gatcgccata caaccagcaa tcatcatccc ggcacgccag
     2401 ccgtaatgta gtgcagccgc tgccatcaca atgggaatga gtgcgccgcc gacgttatgc
     2461 gccgtgttcc ataatgccca ccaaccgccg cgctcggtac gagaatacca ggcggttaat
     2521 agacgcgcac acaccggtga accccagccc tggaaaaagg cgttcagcac ccagagtacg
     2581 gcaaacgccc atagcgacgt cgagaagcca aacagaatgt tgataatgcc cgtggcgata
     2641 agccctatcc ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac
     2701 ttcgacacgc cgtaagtaat gtaaaacagg gtcgctaaca ggccgatatc gctacggctg
     2761 agcacgccgt tagcaaggat ttctggtacg gcggcgttaa aacttttccg cgtgaagtaa
     2821 aacagcgcgt aacccagcca gatggtcagc agaatatgcc gacgccagta gcgatagcgt
     2881 gcatcaattt catgtttgtc agtcattaat ggcgcatcgg caggcgcttt cagaaacggc
     2941 aacatcatcg aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc
     3001 agacaggaaa tgtgtaatgt gccgcccagc gccgttacgc gctcgcgcat tccggtgagg
     3061 ccaaaacctt gttgcccgga acccggcggc aaaccgctgc catcgtcttc aatcaccagc
     3121 atcaaccgtt catcctgctg ccagccttgc agcgtaaccg cgctggcatc ggcatgtttc
     3181 acaatgttgt tcagcccttc ctggcagacg cgaaacagcg tcacgcgctg gttttcgctt
     3241 aacgctgact catcgatacg ccattcgaga tggctgacaa tgccgcgccc ttccagctcc
     3301 atttcccgca tcagtgagcg gatggcctgc tccagggtga gatcatccag ctggcgcgga
     3361 cgtaaccgcc ctaacaaacg gcgcaccgca tcgtaaacac ccagcgacag ttgttcgatg
     3421 agctgcccgc tctgcttcac gctggcgtta tctgccgcca gtcgctggac aatgcccgcc
     3481 tgagtacgaa tggcagtgat ggtctgacct atatcatcgt gcagctcacg cgccacatca
     3541 cggcgcacgc tctcttcggt ttctaacaaa cgttcagcca gatgctgatt gcgcgccagt
     3601 tctttttgca gcgactggtt aagttcacgc aaccgctgga tgccagcgcc aagcaacaac
     3661 cctgtcagac tttgcaccag cagcgagagc aataagtcca ccggatgatc gcgccaggtt
     3721 tgactggcga tcagcgcgat ggcgttcatc aacgtcgcaa tcagcgcccc ttgccaacca
     3781 tagtgccagg ccagcgcgat aatcggcagc gccagacaga atggcgtaaa gcgcgacagt
     3841 tcggccggca atcccaactg gagccagaga ctgataacaa acagcagcaa gtaccagacc
     3901 agatgtcgcc cgcgccagtt gatcggttga gaaactaacg acgggccgag cggcagccag
     3961 gtgttattgg cgagatagtg ccagaacacc agacatatag gggccagcgt caggccgcca
     4021 gttaaagtca gtagtagcgc attccacgac tctttgccgt gccaaagcca gggcagcgac
     4081 tgcaacaacg ccgccgccgt cagggccgcc ccctgtaaca gcaaggtgcg ccagtcacgt
     4141 tgatggcgat agcgcgagat cagcgcaacg ggtagtaacg tcagtaaact accgatcatc
     4201 aataaggaaa aatgggttaa accgaccgcc tgcgttagcc agtaaatcag cagccactcc
     4261 gcgcccagca gcaccggcca gtatccgcgc gggcattgca gcattagccc cagacgcaga
     4321 ccaaacggaa ataacagcac cgccatatca gggcgctcaa ccaggtgcag gctgatactc
     4381 cacaagcaaa accatgcggc agagaagata aaaaagcagg caataacggt aattaagcgg
     4441 gagaacaacg tcttcatcac cagccatcaa acatgcggcg cgccagttca acgtcgttac
     4501 tgacacccag tttttccatc aggttggcac gatgtacatg cacagttttt ggcgacaaac
     4561 ctagttcggc ggcaatctct ttcaccgcca ttccttgcgc cagtttttcc gccacctggc
     4621 gttcacgttt ggttagcggg tcctggcgac cggatgccag tttaatggca atatccggcg
     4681 tcagataaca gccgcccgtg gcaaccgtat gcaccgcaga aatcagttca tccgggctac
     4741 agcgtttgga gagaaagccg cgcgcccccg cgttaagcgc ctgctcaacc agcgccggac
     4801 tgtcatgaac ggagagcata atcgtcgcca tacctttcgg cagctggctt agcagctcca
     4861 gaccggagat atcgggcatg gagatatcgc aaatacacac ctgcacaccg cgccccggca
     4921 gccccgccag cgcctcgcgc cccgaaccaa actcggcaac tacttgcaaa tcaggttcca
     4981 gccccagcaa ctgcgcaaag ccggagcgga cgataaggtg atcgtctata agggcaacgg
     5041 tgatcatgat tgggtcctgg cggataaaaa acgcgcatac cttaacgata agcgcgatgt
     5101 tgttcaagtt ggatatgggg tatgtattgg cttaatgatg ttggtcttat cgtcaaaacg
     5161 cttacctgaa aggtaagcgt ttggtacgtg ttattgcgaa aaagcgaatt atttgtaggt
     5221 tgcgattgca atattgcctg agaaatttct cctcctgttg cggaaatcgt tttcaggtaa
     5281 caaagagtca tccagaaacc tataatcttc agcggaacgc tcattaagag agtcatccac
     5341 tgagtcgtta tgaatataat cctcaccgta tgttcgataa atgaaattag caatattaaa
     5401 atcgttacca gcttcattta tttcggcttt tacaatataa tgagtttgtg tgaatgtatg
     5461 ctgtgaaaag tcagcaaaaa gaaaatttgt ttttcccatc aaagcacaaa accctgggtt
     5521 actattaaac ccggcagaaa tagaattgct tgcctgtatt gcattaatat cccccctatc
     5581 aagcgcgaca ggaaatgcct gtgaacaagc ataatacagg ttacctattc ctgaaatact
     5641 ggagaggcaa gaaataatat tttctctggt ttttttctca acaccatctt tctgtagaga
     5701 gtttctcaca atattacatt catctggcgt aaatacaatt tcactaaaaa gtttttcgaa
     5761 atctttatat tttctaacac ctccatgacc agacaaagcc atggttttac tcctcagtaa
     5821 ttcatggata gtgctattta ttttaactcc acaacatgca ataaatgagc tagcctctcc
     5881 aacatgtctt gatgcatcag caagaagtgc actaaactct ccctcccgaa catctagttt
     5941 ttttatgtct gatttataac tacaatcatc tggaattaat ttatctggaa tcaattcata
     6001 ttggattgat ttatctggag ttgattgagt ttgttcatat aacgcttttt ttaattggtg
     6061 ctcagcatca gtaactccac tgctgagttc atttttaaaa ttattattaa caacttctaa
     6121 cttaagagca tttgcagcaa ctgttacttt tgagagttga tacttttcca atacatcgtg
     6181 ctctgataat aaccctataa actctttcac atttcgacca ctaaaatccc tgtccctact
     6241 ctttttttcc aaactcttgc catttaactc acgatacaca tcagtgactt taaatttatc
     6301 actgccggtc tgttcaattt ttatatagct atccatgcca gtggtagcga tccatgtttt
     6361 ataattcgca ttcgctgatt caggcgtaac atgaaaataa gaaaccaatt taccactgat
     6421 atcatgccga tagatatgaa aagaatctaa aatgtataat aatatatttc ggaaaacttc
     6481 acgaaaagtg gcatctttag ataatgttct acaattatcg agcaggccgc tttgttccat
     6541 cgcatcaaat gcagcaatat taccctcgat actatctctt agactctggc tcaggccctg
     6601 actctgagtt tgactctgaa actggctatt atcattctct gtttccgttg gatatgtata
     6661 actcgtcaca ctaaccatag taacctcgca atatgacacc ttgcatattt tgacatttta
     6721 gaagcatcaa tggttagcat gataaatatg tcaatgctaa ccgctatatt tcatttattt
     6781 caataataag accatgtgtc gttaattttt aacgattttc ccaatgcaat tgcatcacta
     6841 attctactta ttcactatca aaaatcgctc attttttcaa aaagactgtt tatacacaga
     6901 cggttaacaa tacgcgttca ccttaatgat aaacgcgatt tatcaggcaa aaatcataaa
     6961 tcagcagttg ttgcggcacc gaaaaatgta aaagtgactt tatcgatttt ggagttttgt
     7021 tcgtcgaccc taccggtagt aaaataacga gaacactgaa gcttaacttc atttccaaca
     7081 ttattaaccg ccatcgaata catcttct
//
LOCUS       DAYPHN010000203         7057 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000203 DAYPHN010000000
VERSION     DAYPHN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.203"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(51..1364)
                     /locus_tag="ACG0BW_004324"
                     /pseudo
     CDS             complement(51..1364)
                     /locus_tag="ACG0BW_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            1547..1768
                     /locus_tag="ACG0BW_004325"
     CDS             1547..1768
                     /locus_tag="ACG0BW_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFK7261209.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            1768..2148
                     /locus_tag="ACG0BW_004326"
     CDS             1768..2148
                     /locus_tag="ACG0BW_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFK7261210.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            2153..2332
                     /locus_tag="ACG0BW_004327"
     CDS             2153..2332
                     /locus_tag="ACG0BW_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HFK7261211.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            2360..2638
                     /locus_tag="ACG0BW_004328"
                     /pseudo
     CDS             2360..2638
                     /locus_tag="ACG0BW_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
     gene            2643..3056
                     /locus_tag="ACG0BW_004329"
                     /pseudo
     CDS             2643..3056
                     /locus_tag="ACG0BW_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(3006..3341)
                     /locus_tag="ACG0BW_004330"
     CDS             complement(3006..3341)
                     /locus_tag="ACG0BW_004330"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HFK7261212.1"
                     /translation="MAPDGDMPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKI
                     VATPQTPVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHR
                     VRVFPVSVF"
     gene            complement(3551..4531)
                     /locus_tag="ACG0BW_004331"
     CDS             complement(3551..4531)
                     /locus_tag="ACG0BW_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HFK7261213.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     gene            4775..6178
                     /gene="mmuP"
                     /locus_tag="ACG0BW_004332"
     CDS             4775..6178
                     /gene="mmuP"
                     /locus_tag="ACG0BW_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HFK7261214.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            6165..>7057
                     /gene="mmuM"
                     /locus_tag="ACG0BW_004333"
     CDS             6165..>7057
                     /gene="mmuM"
                     /locus_tag="ACG0BW_004333"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HFK7261215.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRT"
BASE COUNT         1565 a         1689 c         1958 g         1845 t
ORIGIN      
        1 ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat caccatctgt
       61 tcgatatggg catcggtcag gatattgttg ttggtctctt ttttgaatag ttcgctggca
      121 tcaataaact gaactttggt atccgtttta tgtttagaca acaccagaat attgacggca
      181 atggtggtgc caaagaacag gttcggtgcg agtgaaatca cggtttcgac atagttattg
      241 tcaaccagat actgacggat tttctgctcc gcgccgccac ggtaaaaaat gcccgggaag
      301 cagacaatcg cagcacgacc tttggcagaa agatagttca gcgcatgtaa tacaaacgca
      361 aagtcagctt tggatttggg ggccagaacg ccagccgggg caaaacgttc atcgttaatc
      421 agcgtcgggt catcgctgcc aatccatttc accgaatacg gcgggttaga aacgatggca
      481 tcaaacggtt tttcatctct gaagtgcggt tcagtcagcg tattgcccag cttgatatca
      541 aacttgtcgt agttgatgtt gtgcaaaaac atgttcatac gcgccaggtt ataggtcgta
      601 tggttgattt cctgaccaaa aaaaccttct tcgatgatat ggttatcaaa ctgttttttc
      661 gcctgcaaca acagtgagcc ggaacccgct gccgggtcgt agattttgtt aacgctggtc
      721 tgcccgtgca tagccagttg tgcaatcagc ttggagacgt gctgcggtgt aaagaactca
      781 ccgcctgact taccggcatt tgccgcatag ttagaaatca ggaactcata ggcatcaccg
      841 aacaggtcaa tctgatgttc gttgaagtca ccaagtttta acccttcaac ccctttcaga
      901 accgcagcca ggcgggcatt tttatcttta acggtgttac ccaggcggtt actggtggta
      961 tcgaaatcag caaacaaacc tttgatgtca gcttctgaag gataaccgta agcagaactt
     1021 tcgatagcaa cgaagatgct gtttaaatct gcattcaatc tgtcattggt atttgctttc
     1081 gcagctacgt tgcagaaaag ctggcttggg tagatgaagt agcctttggt tttgatggca
     1141 tcgtctttaa tgtcatcagt aattacgctg tcatccagtt tcgcataaca gatactgtca
     1201 tcaccggctt caatataact ggaaaaattt tcgctgataa aacggtagaa aagtgcgccc
     1261 agaacgtatt gcttaaaatc ccatccatcg accgaacccc tgacatcgtt agcaatttgc
     1321 cagatttgac gatgaagctc tgcacgttgt tgaatacttg tcattttcat ccacttattt
     1381 caggcttatg taattggcgg tgattctaca gcaacttgga tgctttagca gttcggacat
     1441 taggctacga atgacctgcc tagaggtttg ttaagccgca aagtgctggt gctttatgcc
     1501 tgtgaagttt ataattgtgt acacataacg agtacacgag gtgtttatgc aatccattaa
     1561 cttccgtacc gcgcgcggca acctttctga agtgctcaac aatgttgagg ccggggaaga
     1621 ggttgaaatc acccgcagag gccgtgagcc agcagtaatt gtcagcaagg ctactttcga
     1681 agcctacaaa aaagcggcgc tggatgctga atttgcatcc ctgtttgaca ccctggactc
     1741 caccaacaag gaactggtta accgataatg aggcatatat caccggaaga acttattgcg
     1801 cttcatgatg cgaatataaa ccgctacggc ggcctgccgg gaatgtctga tccgggcagg
     1861 gcagaggcca ttatcgggag agttcaggcc agagttgcct acgaagagat caccgacctt
     1921 ttcgaagtct ccgccaccta cctggtggct acagcgagag ggcatatatt caatgatgcc
     1981 aataagcgta ccgcgctaaa cagtgcgctg ttatttctac gccgtaacgg ggtgcaggta
     2041 tttgattcac ctgaactggc agaccttacc gtaggggctg cgaccggaga gatatctgta
     2101 tcttctgtcg ccgacacgtt acgtagattg tatggttctg cggagtagat taatggcacg
     2161 caaatacaac aaattgtccc gtgaagcgtt aaagatgctt cttgatggcg tgagtcgccg
     2221 cgaggtaaag caatacctgg ctggtaagca aattggtgcc aggaccgcta ttgctgtgtt
     2281 atgccgtcag gaaatggttg tgcttaaaca gagaatgccg ggcagcagat aaagcccaat
     2341 cagtgatgaa aggtgtgatg tgaaagccgt aattactccc tttgtacaaa aagagcttgg
     2401 cgtcgccaca ttcaaagtgg atcaggaagt cagaaagctg gtggaggctg gccgtaaatt
     2461 tattatggag ccggtaccgc gtgagttaat cgagcacatg gacgacggcc tcgttgtttc
     2521 cgagcaaact atggcaacaa atgaggcgtt gcagccgttt tttaacagcg atgaactgtt
     2581 tcgccgtatt ggtggaattg actcgctggt agcgtggttg cgcaggaaag aggggcaggt
     2641 caggctgggc catgtcgttc tcgccggaca gcaggcttac catgaaagca ctggaaatgg
     2701 catgggaaac ccgtggtaag cccgtcgggg tgatgttcca cagcgatcaa ggcagtcatt
     2761 atacgagcag gcagttccgg cagttactgt ggcgataccg gatcaggcag agtatgagtc
     2821 ggcgtggaaa ctgctgggat aacagcccaa tggagcgctt cttcaggagt ctgaagaacg
     2881 aatgggtgcc agcgacgggc tatgtaagtt tcagcgatgc agctcacgcc ataacggact
     2941 atatcgttgg atattacagc gcactaagac cgcacgaata taatggtggg ttaccaccaa
     3001 acgaatcaga aaaccgatac tggaaaaact ctaacgcggt ggccagtttt agttgaccac
     3061 aacacaaaat caattaatcg aacatgagta tgcttggtca cccattgttc ttgctccatt
     3121 accgagtatt caacaggcac accttcgatt aaatattggt gaaagagttt gttgttttta
     3181 atcagtacag gcgtttgcgg tgtggcaacg attttcgcta cttcgattaa ggtgtcactg
     3241 ggcacatttg ggtttaaggt ttccaaggca gagactaaac gcgcctgtag cgtgacttgc
     3301 tgatagtttt cacgttcagg catatcacca tctggtgcga tgtcatagcc attttggtat
     3361 tggtaaccta gctctttaaa ccaatcgagg cattgttgtt ctagggaagg tgcgaataag
     3421 cggggaaatt cttctcggct gactcagtca tttcatttct tcatgtttga gccgattttt
     3481 tctcccgtaa atgccttgaa tcagcctatt tagaccgttt cttcgccatt taaggcgtta
     3541 tccccagttt ttagtgagat ctctcccact gacgtatcat ttggtccgcc cgaaacaggt
     3601 tggccagcgt gaataacatc gccagttggt tatcgttttt cagcaacccc ttgtatctgg
     3661 ctttcacgaa gccgaactgt cgcttgatga tgcgaaatgg gtgctccacc ttggcccgga
     3721 tgctggcttt catgtattcg atgttgatgg ccgttttgtt cttgcgtgga tgctgtttca
     3781 aggttcttac cttgccgggg cgctcggcga tcagccagtc cacatccacc tcggccagct
     3841 cctcgcgctg tggcgcccct tggtagccgg catcggctga gacaaattgc tcctctccat
     3901 gcagcagatt acccagctga ttgaggtcat gctcgttggc cgcggtggtg accaggctgt
     3961 gggtcaggcc actcttggca tcgacaccaa tgtgggcctt catgccaaag tgccactgat
     4021 tgcctttctt ggtctgatgc atctccggat cgcgttgctg ctctttgttc ttggtcgagc
     4081 tgggtgcctc aatgatggtg gcatcgacca aggtgccttg agtcatcatg acgcctgctt
     4141 cggccagcca gcgactgatg gtcttgaaca attggcgggc cagttgatgc tgctccagca
     4201 ggtggcggaa attcatgatg gtggtgcggt ccggcaaggc gctatccagg gataaccggg
     4261 caaacagacg catggaggcg atttcgtaca gagcatcttc catcgcgcca tcgctcaggt
     4321 tgtaccaatg ctgcatgcag tgaatgcgta gcatggtttc cagcggataa ggtcgccggc
     4381 cattaccagc cttggggtaa aacggctcga tgacttccac catgttttgc catggcagaa
     4441 tctgctccat gcgggacaag aaaatctctt ttctggtctg acggcgctta ctgctgaatt
     4501 cactgtcggc gaaggtaagt tgatgactca tgatgaaccc tgttctatgg ctccagatga
     4561 caaacatgat ctcatatcag ggacttgttc gcaccttccg gaggcgttat gagctggcgg
     4621 ccctttttgt atctgattat taatccccac ccgctattaa gcgcccggcg cgggcatctg
     4681 cgtctggtgc agggttgact ttgcattctg ttaacaaacg cggtataaca aaccttcttt
     4741 ggatgtttag atgtccatac gtttagaagg ttatatgcaa acaacacaac aaaatgcgcc
     4801 actgaagcgc acaatgaaaa cgcgtcacct gattatgctt tccttgggcg gcgtgattgg
     4861 cacaggatta ttcttcaata ccgggtacat catttccacc actggagcgg cgggaacgct
     4921 gctggcctat ctgattggtg cgctggtggt ctggctggtt atgcagtgtc tgggcgagct
     4981 gtcggtcgcg atgccggaga ccggagcgtt tcacgtttat gccgcgcgct atcttggtcc
     5041 ggctaccggg tataccgtgg cctggcttta ctggctgacc tggaccgtgg cgctgggttc
     5101 gagctttacc gccgctggat tctgtatgca gtactggttt ccacaggtgc cggtatgggt
     5161 ctggtgcgtg gtgttctgcg cgattatttt tggtctgaat gttatctcca cgcgcttttt
     5221 tgccgaaggg gagttctggt tctcgctggt caaagtggtc actatcatcg cctttatcat
     5281 cctcggtggg gcggcgattt tcggctttat tccgatgcag gatggctcgc ccgcgccggg
     5341 gctgagtaat atcacggcag aaggctggtt cccgcacggt ggcttaccga ttttgatgac
     5401 tatggtggca gtgaactttg ctttttcggg taccgagctt atcggcattg ccgccggtga
     5461 aacggaaaac ccgcgcaaag ttatcccggt agcgattcgt actaccatcg cgcgactgat
     5521 tattttcttt atcggcaccg tgtttgtgct ggcagcgctg atcccgatgc agcaggcggg
     5581 cgtggagaaa agcccgtttg tgctggtatt tgagaaagta gggatcccgt acgccgctga
     5641 tatttttaac ttcgtgatcc tgacggctat tctttctgca gcgaactccg ggttatatgc
     5701 ctccgggcgc atgctgtggt cgttgtcgaa tgaacgtacg ctaccggcct gttttgcgcg
     5761 agtaacgaaa aacggcgtgc cactgacggc gctgtcggtc agtatgctcg gtggtgtgct
     5821 ggcgctgttt tccagcgtgg tggccccgga cacggtattt gttgcgctgt cggcaatctc
     5881 cgggtttgcg gtggtagcgg tgtggctgag tatctgcgcc tcgcattttg tttttcgtcg
     5941 ccgtcatctg caacaaggta aggcattgag tgaattacat tatcgcgcgc cgtggtatcc
     6001 gctggtgcca gtattaggtt ttgtgctgtg cctggtggcc tgtgttgggc tggcattcga
     6061 tccagcgcag agaattgcgt tgtggtgcgg gttaccgttt gttgcgttgt gctatggtgc
     6121 ttatttcctt actcaacccc gaaacgcaaa acaggagcca gaacatgtcg cagaataatc
     6181 cgttacgcgc tcttcttgat aaacaggata tcctgctgct ggatggcgcg atggcgacgg
     6241 agctggaagc gcgagggtgt aacttagccg acagcctgtg gtcagccaaa gtgctggtag
     6301 aaaacccgga gcttatccgc gaagtgcatc ttgattacta ccgggcgggg gcgcaatgcg
     6361 cgatcactgc cagctatcag gcgacgccgg cgggcttcgc cgcgcgaggt ctggatgaag
     6421 cgcagtcgaa agcgctgatt ggcaaaagcg tggagctggc gcgtaaagcc cgtgaagcgt
     6481 atctggcaga gaacccgcag gcgggaacgc ttctggtggc gggatccgtc gggccttacg
     6541 gcgcgtatct ggcggatggc tctgaatacc gtggcgatta tcattgtagc gttgaggcat
     6601 ttcaggcgtt tcatcgcccg cgcgtggaag ccttgctgga tgccggggcc gatctgctgg
     6661 cctgcgaaac cctgccgaat ttttccgaga ttgaggcgtt ggccgagctg ttgaccgcat
     6721 atccgcgtgc gcgggcgtgg ttctcattta ccctgcgcga cagcgaacac ctgagcgacg
     6781 gtacgccgct gcgtgacgtg gttgcgttgc tggcgggtta tccgcaggtg gtggcgctag
     6841 gcattaactg tattgcgctg gaaaacacca ccgctgcgtt gcagcattta cacggtttaa
     6901 cggtgctgcc gctggtggtg tatccgaact cgggcgagca ttacgatgcc gtgagcaaaa
     6961 cctggcatca tcacggtgaa cattgcgcgc agctggcgga ttatctgccg cagtggcagg
     7021 ccgctggcgc acggttgatt ggcgggtgct gtcgcac
//
LOCUS       DAYPHN010000204         7019 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000204 DAYPHN010000000
VERSION     DAYPHN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.204"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..823
                     /locus_tag="ACG0BW_004334"
     CDS             <1..823
                     /locus_tag="ACG0BW_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFK7261216.1"
                     /translation="RYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPVIISFG
                     TGFVGNTSFEDFSHMMVSMAKKATVPVITHWDHGRSMEIIHNAWTHGMNSLMRDASAF
                     DFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAEFVERT
                     GCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDTDIKTAISLG
                     IAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGKAE"
     gene            828..1775
                     /locus_tag="ACG0BW_004335"
     CDS             828..1775
                     /locus_tag="ACG0BW_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFK7261217.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRVGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSFVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            1785..2765
                     /gene="ydjG"
                     /locus_tag="ACG0BW_004336"
     CDS             1785..2765
                     /gene="ydjG"
                     /locus_tag="ACG0BW_004336"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFK7261218.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDQQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMESELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            2902..3660
                     /locus_tag="ACG0BW_004337"
     CDS             2902..3660
                     /locus_tag="ACG0BW_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFK7261219.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            3778..5136
                     /locus_tag="ACG0BW_004338"
     CDS             3778..5136
                     /locus_tag="ACG0BW_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261220.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSVRLPPLTLNQSNSKANVINGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(5229..5870)
                     /gene="pncA"
                     /locus_tag="ACG0BW_004339"
     CDS             complement(5229..5870)
                     /gene="pncA"
                     /locus_tag="ACG0BW_004339"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFK7261221.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(5881..6897)
                     /gene="ansA"
                     /locus_tag="ACG0BW_004340"
     CDS             complement(5881..6897)
                     /gene="ansA"
                     /locus_tag="ACG0BW_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFK7261222.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
BASE COUNT         1846 a         1485 c         1776 g         1912 t
ORIGIN      
        1 cagatattgg gaaaacgatg ccacaaataa acattatgca attgcccatt ttaacgtatg
       61 gaatgcagaa atgttgatgg gcgttatcga cgcagccgaa gaagcgaaat caccggttat
      121 tatttctttt ggtacaggtt ttgtcgggaa cacctcattt gaagatttct ctcacatgat
      181 ggtatcaatg gcaaaaaaag caacggtgcc ggtaataact cattgggatc atggtcggag
      241 tatggaaatt attcataacg cctggactca tggtatgaat tcattaatgc gtgatgcttc
      301 cgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt tcttccatcc
      361 gctgggtatt ccggtagagg cagaattagg gcatgtcggt aatgaaaccg tttatgaaga
      421 agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac
      481 gggctgtgac tcattggctg tcgccatcgg caaccaacat ggggtttata cgtcagagcc
      541 acaattgaat tttgaggtcg tcaaacgcgt acgcgatgcc gtttctgttc cgctggtttt
      601 gcacggcgca tcggggatca gtgataccga cattaaaact gcaatttcgt tgggtatcgc
      661 gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtagcagtga aagaaaacca
      721 ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt
      781 ggaaaaaatt aaactgttcg gttctgatgg caaagcggaa taatgatatg gataatctcg
      841 acgttatttg tataggtgcc gctattgttg atattccatt gcaaccggtc agtaaaaata
      901 tctttgatgt ggattcttat cctcttgaaa gaatcgcaat gaccaccggg ggtgatgcaa
      961 taaatgaagc aacaattatt tctcgtctgg gccatcgcac agcattaatg agtcgtgttg
     1021 gtaaagatgc cgcaggacaa tttattctcg accattgccg caaagagaat attgatattc
     1081 aaagcctgaa gcaggacgtc agcatagata cctctattaa cgttggactg gtgacggagg
     1141 atggcgagcg gacgtttgtc acaaaccgta acggcagtct gtggaaattg aatattgacg
     1201 atgttgattt tgcgcggttt tctcaggcga aattattatc actggccagt attttcaaca
     1261 gtcctctatt ggatggtaaa gcgctaacag aaatttttac gcaagctaaa gcccggcaga
     1321 tgattatctg tgccgatatg atcaaaccac ggttgaatga aacgctggat gatatttgcg
     1381 aagcattgag ttttgtcgat tatctgtttc ctaattttgc cgaggcaaaa ttactcaccg
     1441 ggaaagagac actggatgaa attgctgact gctttcttgc gtgcggcgta aaaacggtgg
     1501 tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg tgacatgacg atgaaggtgc
     1561 cggcggtcgc aggaataacc gccatcgaca ccattggcgc gggcgataac tttgcttcag
     1621 gttttattgc ggcactgtta gaaggcaaaa atctgcgtga atgcgcacgc tttgccaatg
     1681 caacggcggc tatctcggtt ctaagcgtcg gtgccaccac cggcgtaaaa aacagaaagc
     1741 tggtcgaaca attgctggaa gaatacgaag gataatgaag gcaaatgaaa aagatacctt
     1801 taggcacaac ggatattacg ctttcgcgaa tggggttggg gacatgggcc attggcggcg
     1861 gtcctgcatg gaatggcgat ctcgatcagc aaatatgtat tgatacgatt cttgaagccc
     1921 atcgttgtgg cattaatctg attgatactg cgccaggata taactttggc aatagtgaag
     1981 ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca ggttgtagta gaaaccaaat
     2041 gcggcattgt ctgggaacga aaaggaagtt tattcaacaa agttggcgat cggcagttgt
     2101 ataaaaacct ttccccggaa tctatccgcg aagaggtaga agccagcttg caacgtctgg
     2161 gtattgatta catcgatatc tacatgacgc actggcagtc ggtgccgcca ttttttacgc
     2221 cgatcgctga aactgtcgca gtgctcaatg agttaaaggc cgaagggaaa attcgcgcta
     2281 taggcgctgc taacgtcgat gctgaccata tccgcgagta tctgcaatat ggtgaactgg
     2341 atattattca ggcgaaatac agtatcctcg accgggcaat ggaaagcgaa ctgctgccgc
     2401 tatgtcgtga taatggcatt gtggtacagg tttattcccc gttagagcag ggattgttga
     2461 ccggtaccat cactcgtgat tacgttccgg gcggcgctcg ggcaaataaa gtctggttcc
     2521 agcgtgaaaa catgctgaaa gtgattgata tgcttgaaca gtggcagcca ctctgtgctc
     2581 gttatcagtg cacaattccc actctggcac tggcgtggat attaaaacag agtgatttaa
     2641 tctccattct tagtggggct actgcaccgg aacaggtacg cgaaaatgtc gcggcattga
     2701 atatcaactt atcggatgca gacgcaacat tgatgaggga aatggcagag gccctggagc
     2761 gttaaatact caacacgatt cacctctgtt tctgtgaaaa ctgtgatgaa aatctgtgat
     2821 tcaaacaggc tattttgaaa gtaaacatcg gttatgcgat aatcacgcta atgtgatgta
     2881 aaaagataca ggggtgtaat cgtggcggca aaagacagga ttcaggcaat taagcaaatg
     2941 gttgccaacg ataaaaaggt gacagtctca aatttgagtg ggatttttca ggtaaccgaa
     3001 gaaaccattc gccgcgatct tgagaagctg gaagatgaag gctttttgac cagaacctat
     3061 ggtggtgctg ttttaaatac agcgatgttg acggagaata tccattttta taagcgtgct
     3121 tcatcgtttt atgaagagaa gcagcttatt gcacgcaagg cactaccctt tatcgacaat
     3181 aaaaccacca tggcagccga ttccagtagt accgtaatgg aattgctcaa attgttacag
     3241 gaccgtagtg acctgacgtt gctaaccaac tcggcggaag cgattcatgt tctggctcag
     3301 tcagaaatta aagtcgtttc aacgggtggg gaactaaaca aaaatacact ttccctgcaa
     3361 ggaagaatta ctaaagagat catcagccgc taccatgtcg atatcatggt aatgagttgt
     3421 aaaggtctag atattaacag cggcgcgctg gactctaacg aagcggaagc tgaaatcaaa
     3481 aagacaatga tccgtcaggc gacagaggta gcgttattgg ttgatcactc taagtttgat
     3541 cgcaaagctt ttgtccagtt agctgacttt agtcatatta attacataat aactgataaa
     3601 tcaccgggtg cagagtggat agcattttgc aaagacaata atatccagct ggtgtggtaa
     3661 tttaagcaca cgttgtcaat attatttgtt cctgcgcctg gaaaataaaa tgatgataaa
     3721 gatgcggcaa gtgaatttgc cgcaattgct acctttttag caaatccgag gcacctaatg
     3781 gaacaatatg atcaaattgg cgcaagactg gaccgcttgc ctttggcccg gtttcattat
     3841 cgtatatttg gtattataag ctttagtctg ttattaacgg gttttttgag ttattcaggc
     3901 aatgttgttt tagcaaagct ggtaagcaat ggatggtcaa ataatttcct caatgccgcc
     3961 tttacctcgg cattaatgtt tggttatttc atcggctctc ttaccggtgg gttcatcggt
     4021 gattattttg ggcggcgcag ggcttttcgc ataaatcttc tcatcgtcgg tattgcggca
     4081 acaggggccg cttttgtccc tgatatgtac tggctcatct tctttcgctt cctgatgggc
     4141 acaggaatgg gggcgctgat tatggttggc tatgcttcgt ttacggagtt tatccccgcg
     4201 acggtgcgtg gaaaatggtc cgcgcgactc tcatttgtcg gtaactggtc gcccatgttg
     4261 tctgcggcga taggcgtggt ggttatcgct ttttttagtt ggcggataat gtttctgctg
     4321 ggtggtattg gcatactgtt agcctggttt ctctcaggta aatactttat tgagtcgcct
     4381 cgatggttgg cgggtaaagg acaaatcgca ggtgcagaaa gccaacttcg tgaagtagag
     4441 cagcaaattg aaagagagaa gagtgttcgt ttacccccgc ttactttgaa tcagagcaac
     4501 agcaaggcta acgtaatcaa tggtactttc tggctgctgt ttaaagggga aatgttacga
     4561 cgtacattag tcgcgattac tgttttaatt gcaatgaata tttcacttta taccatcacc
     4621 gtatggatac cgaccatatt tgttaactcc ggcattgatg tcgataaatc aatattaatg
     4681 actgctgtta ttatgattgg cgctccggta ggaatattta ttgcggcatt aattattgat
     4741 catttccctc gtcgattatt tggttccgcc ttgcttatta ttattgccgt gttaggctat
     4801 atctattcaa ttcagactac agagtgggcg attttaatct atggcctggt gatgatcttc
     4861 tttttataca tgtatgtttg cttcgcgtcg gcggtttata tcccggagct ttggccaacg
     4921 catttacgcc tgcgcggttc aggtttcgtt aatgccgtcg gacggatcgt cgcggtcttc
     4981 acgccttatg gcgttgcggc actattaacg cattatgggt caatcacggt gtttatagtg
     5041 cttggcgtga tgttagtgct ctgtgcgctg gttctctcca tttttggcat cgaaacgcgg
     5101 aaggtgtcat tggaagagat ttctgaggtg aattaaatct ttcgttgatg gcgtcaattg
     5161 ccagatgtac cactgacgtg ttttatctgg cctacaatgg cagtacagaa ttgtaggcca
     5221 gcataaaatt actcctgcgt ctcttcccag tctgccagcg tatatagcgt tgccccagct
     5281 gctgacatct ccataaacgc gtgcgcactg tcctgtggct ggatattcac accacgacaa
     5341 ccatcggtaa tcacattaac cttataacct aactgtaacg cgtccagtac ggtaaacttc
     5401 acgcaatagt cagtagccag gcccataatg atcaattcat cgatttcatg atcgcgtaac
     5461 cagtcatcga gcgtggtttt ctgccgacgg ccgttatcaa aaaaggcact gtaactgtca
     5521 actaaaggat tttcgccttt atggaacacc gctgcgatcg ctttttggtt cagtaacgga
     5581 tgtaattgtg cgccttcact gttctgcaca cagtgatctg gccagaaggt ttgtggcaaa
     5641 ccgtcgagtt ggcctggcgt ataaggctct acaccgtgct gactggcaaa actgccgtga
     5701 ttcgccgggt gccagtcctg actggcgata accgcttcac cgcgcgactg gcaccagtca
     5761 atcaggcggt tagcgacatc caccgtactg tcaccttccg gcacggcgag cgcgccacca
     5821 gcacagaaat cattttgtaa atcgaccagt aacagggcgc gagggggcat tgcagtctcc
     5881 ttaatcatcc ggcgtcagtt cgccgcgcag gttttggctc atggccttgc gaatggtttc
     5941 agtatccagt tcctggctaa gcaggtaatg cagtttggtt agtgtggctt ctacagtcat
     6001 atctgcaccg ccaataacgc cggcatgggc gagggcgtta ccggtggcat aaccacccat
     6061 gttcacttta ccggacatac attgtgtcag gttgaccacc acaataccgc gatcgctggc
     6121 ttcttgtaat tcctgcagga aggctttgtt ttgtggcgca ttacccacgc catatgagcg
     6181 cagaatcaat gctttcaccg gttggcgcag aaaattgcgc acgacgtcag cagaaatccc
     6241 cggataaatc gtcactacgc caatgggttg tggggtgatt ggatgaacga tcaattcccc
     6301 ttcaccgtgc ggggcgggtg gcgtattcaa acgacgaata tggatacctg cttccagtaa
     6361 cggaggaagg tttggagagg caaacgcatc aaaaccatcg gcatgggctt tggtggtacg
     6421 gttgccacga taaagtcggt tattaaaaaa gagcgttact tcgttgatcg gataattcgc
     6481 cgcaacgtac aacgcattca gcagattaat ttgtccgtca gagcgtaact cagctagcgg
     6541 gatttgtgac cctgtcacaa tgaccggttt accgagattc tcgagcatga acgacagcgc
     6601 agaggcggta tacgccatcg tgtcggtgcc gtgcagaatg acaaaaccat catagtcgtc
     6661 ataatgcgct ttaatatctt cagcaatatg ctgccagtct tctggcgtca tatctgaaga
     6721 gtccatcagc ggcgtatatt catgaatggt gaaatctggc atctccgggc gatggaattc
     6781 cggcatcagc gccagttggc gctgtagatg acctgacacc ggaatataac cctgctcgga
     6841 acgctgcatc ccgatggtcc cgcccgtgta ggcgacgtaa attgatttct tttgcatgat
     6901 attgatatga atgatatcga aagagcaaaa gtatatacgt gaggcagggg gagcgcacct
     6961 gatactatgc ggataaggaa atatggggcg agacagcggg aagaggattc gtcgaggta
//
LOCUS       DAYPHN010000205         6995 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000205 DAYPHN010000000
VERSION     DAYPHN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.205"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..5352
                     /gene="air"
                     /locus_tag="ACG0BW_004341"
     CDS             <1..5352
                     /gene="air"
                     /locus_tag="ACG0BW_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HFK7261223.1"
                     /translation="ADVSTAKIADLVVIKDGSEADGSTANTLQVKVTDAFGNALAGQT
                     VSVMAGNGATVAPTVITEPDGTVEISVTSQTAGTSTVTASINNSSQSRDVTFIADVRT
                     AQIASLEVTQDNAVADGAMANTLRARVTDAFGNALAGQTVSVMAGNGATTAPTVTTQP
                     DGTVEISVTSQTAGISTVTATINNSTLSQNVTFIADVRTAKIADLVVIKDGSEADGST
                     ANTLRVKVTDAFGNTLAGQTVSVLGGNGATTAPTVITGPDGTVESSVTSQTAGISTVT
                     ATINNSSLSRNVTFIADVRTAQIASLEVTQDNAVADGAMANTLRVKVTDAFGNVLAGQ
                     MVSVTAGNSATVASTVTTHPDGTVEISVTSQTAGTSTVTASINSSSQSQSVKFIADVS
                     TAQIAVLEVTQDNSVADGSTANTLLVRVTDAFGNTLAGQTVSVTAGNGATVAPTVITE
                     PDGTVEISVTSQTAGISAVTASINSSSQSRNVTFIADVRTAQIADLAVIKDGSVADGS
                     TANTLRARVTDAFGNALAGQTVSVLADNGATVSPTVITGPDGTVEISVTSQTAGISAV
                     TVSINNSTLSQNVTFIADVRTAKIAELVVSQDNAVADGATANTLRVRVTDAFGNALAG
                     QTVSVLAGNGATTAPTVTTQPDGTVEISVTSQMAGTSAVTASINSSSQSGDVTFIADA
                     STAQIADLVVIKDGSEADGSTANTLRARVTDAFGNALAGQTVSVTADNGATLSPTVIT
                     GPDGTVEISVTSQTAGASTVTASINSSSQSRNVTFIADVRTAQIASLEVRQDNSVADG
                     AMANTLRVKVTDAFGNALAGQTVSVMAGNGATVAPTVITEPDGTVEISVTSQTAGIST
                     VTATINSSSQSRDVTFIADVRTAQIADLVVIKDGSEADGSTANTLRARVTDAFGNTLA
                     GQTVSVLGGNGATTAPTVITGPDGTVEISVTSQTAGISVVTASINSSSQSRDVTFIAD
                     VRTAQIADLVVIKDGSVADGATANTLQVKVTDANGNALAGQTVSVMAGNGATTAPTVT
                     TQPDGTVEISVTSQTAGTSVVTASINNSSQSQNVTFVPGDASQLTSTVETNKSNYTVG
                     ETITITVTLRDAFDNLVTGAASQLAANGVLTVAGTDPSETGSWVESGGVYTTTRMATI
                     ASTNQHANLQLQSWSDGVTSDRYDIQSGSPAQATSTIATDKNAYTAGETITVAVTLKD
                     AHGNLVEGGESLLSGDNVIVEGAVRSGGWSENAGVYTATWSAQMAGDSHHATLKLSEW
                     GSSKQSESYSIHSGAPVQANSAIRTDKSAYIAGEPLTVTVTLRDEFGNPAFGLTSEVI
                     ESYIDSFAVGGATPDSMQWVEQNNGEYTIVWTAWGAEENLVASLKLKTWAAEIKSSLY
                     GIQPGAAAKTQSTIVADKTIYIAGDSITVTVVLKDAQGNFITDGVVQLNEENVQVRNA
                     DPIQGNNWVYNGNGQYQRQYMAHFAEANLNAQLKMAGWSDANYSNNYTIKPGEVSPLG
                     SQLRIREVLVVEGADLPVSALLVDDFGNPVDNGLDLLDDAVYLQNVEKKEGEKWRYVG
                     DGIYERTYMAYQEGENLTSFMEIKGWRIYGQPSYNILPFVEVESLSVNGVKFRATDGF
                     PETGFDGAKFTLLLTHNMKNTDYNWTAGIYGINVDSNGEVTLSVLIRSEVTITGKPKN
                     GKGNDVVFKFKIKKWFTSLGASSSNTWDIINTSCSYGQMPSSLELAQRPSGGVVPRKV
                     GTLWGEYGNLKTYGNAFSSTDYWTSTQLMGVHEKFNPETGISELGTGKSSGLCVEYY"
     gene            complement(5404..>6995)
                     /gene="eilA"
                     /locus_tag="ACG0BW_004342"
     CDS             complement(5404..>6995)
                     /gene="eilA"
                     /locus_tag="ACG0BW_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFK7261224.1"
                     /translation="PKELGVIILLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYA
                     LRKLLHENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLK
                     LHYELVQGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNW
                     RLFIELIHARTHKLIDHQSIDFNPDNPLSVLLSQVINILIEKIPNINFKSINTQQMPS
                     LDSAVMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGL
                     SEQKQAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNS
                     PDVYYYQSLLCFLNGDLARAFNLIEKSIALEPNKMGISILKLIILYYTSPLDNAISFA
                     LNLNSQNTCNNPIITSILAMFMALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKFLI
                     YGASIKNDIMKLLANISTNKINGVILPLIYTVYGKKEYEKRWQQLIKENDLWSNVLLH
                     DPRLLSVKNEFNTIGVMRTSA"
BASE COUNT         1876 a         1559 c         2001 g         1559 t
ORIGIN      
        1 gccgatgtca gcacggcgaa gatcgctgat ctggtggtca ttaaggatgg ttcagaggca
       61 gacggttcga cggcgaacac gctgcaggtg aaggtgaccg atgcgttcgg gaatgcactt
      121 gccggacaga cggtcagcgt gatggcaggc aacggcgcaa cggttgcccc gacggtgatt
      181 acagagccgg acggcacggt ggagatctct gtcaccagcc agacggcagg aaccagtaca
      241 gtcaccgcca gcatcaacaa cagcagccag agccgggatg tgacgtttat cgccgatgtc
      301 aggacggcgc agatcgcgag tctggaggtg acgcaggata acgccgtggc tgacggcgca
      361 atggcgaaca cgctgcgggc gagggtgacc gatgcgttcg gtaatgcgct tgccggacag
      421 acggtcagcg tgatggcagg caacggtgcc acgactgctc cgacggtcac cacgcagccg
      481 gacggcacgg tggagatctc tgtcaccagc cagacggcgg ggatcagtac agtcactgca
      541 accatcaaca acagcaccct gagtcagaac gtgacattta tcgccgatgt caggacggcg
      601 aagatagcgg atctggtggt tattaaggat ggttcagagg cggacggttc gacggcgaac
      661 acgctgcggg tgaaggtcac cgatgcgttc ggtaatacgc ttgccgggca gacggtgagc
      721 gtgttgggag gcaacggcgc aacgactgcc ccgacggtca tcacagggcc agacgggacg
      781 gtggagagca gtgtcaccag ccagacggcg gggatcagta cagtcactgc aaccatcaac
      841 aacagcagcc tgagcaggaa cgtgacattt atcgccgatg tcaggacggc gcagatcgcg
      901 agtctggagg tgacgcagga taacgccgtg gctgacggcg caatggcgaa cacactgcgg
      961 gtgaaggtca ccgatgcgtt cggtaatgtg ctggccgggc agatggtcag cgtgacggca
     1021 ggcaacagcg caacggttgc ttcgacggtc accacgcatc cggacggcac ggtggagatc
     1081 tctgtcacca gtcagacggc gggtaccagt acagtcaccg ccagcatcaa cagcagcagc
     1141 cagagccaga gcgtgaagtt tattgccgat gtcagcacgg cgcagattgc ggtgctggaa
     1201 gtgacgcagg ataactcagt ggcagacggt tcgacggcga acacgctgct ggtgagggtg
     1261 accgatgcgt tcggtaatac gcttgccggg cagacggtca gcgtgacggc aggcaacggt
     1321 gcaacggttg ccccgacggt gattacagag ccggacggca cggtggagat ctctgtcacc
     1381 agccagaccg ccggaatcag tgcggtcact gccagcatta acagcagcag ccagagccgg
     1441 aacgtcacgt ttatcgccga tgtcaggacg gcgcagatcg cggacctggc ggttattaag
     1501 gacggctcag tggcggacgg ttcgacggcg aacacgctgc gtgcgagggt aaccgatgca
     1561 tttggtaatg cgcttgccgg gcagacggtg agcgtgttgg cggacaacgg cgcaacggtg
     1621 tctccgacgg tcatcacagg gccggacggt acggtggaga tctctgtcac cagccagacg
     1681 gcgggaatca gtgcggtcac cgtcagcatc aacaacagca ccctgagcca gaacgtgaca
     1741 tttatcgctg atgtcaggac ggcgaagata gcggagctgg tcgttagcca ggataacgcg
     1801 gtggcagacg gtgcgacggc gaacacgctg cgggtgaggg tgaccgacgc gttcggtaat
     1861 gcgcttgccg ggcagacggt cagcgtgttg gcaggcaacg gtgccacgac tgctccgacg
     1921 gtcaccacac agccggacgg cacggtggag atctctgtca ccagccagat ggcgggcacc
     1981 agtgcggtca ccgccagcat taacagcagc agtcagagcg gggatgtcac atttatcgcc
     2041 gatgccagca cggcgcagat cgcggacctg gtggtcatta aggatggttc agaggcggac
     2101 ggttcgacgg cgaacacgct gcgggcgagg gtgaccgatg cgttcggtaa tgcgcttgcc
     2161 ggacagacgg tcagcgtgac ggcagacaac ggtgcaacgt tgtctccgac ggtcatcaca
     2221 gggccggacg gcacggtgga gatcagtgtc accagccaga cggcgggagc cagtacagtc
     2281 actgccagca tcaacagcag cagccagagc cggaacgtca cgtttatcgc cgatgtcagg
     2341 acggcgcaga tcgcgagtct ggaggtgagg caggataact cagtggcaga cggcgcaatg
     2401 gcgaacacgc tgcgggtgaa ggtcaccgat gcgttcggca atgcgcttgc cggacagacg
     2461 gtcagcgtga tggcaggcaa cggtgcaacg gttgctccga cggtgattac agagccggac
     2521 ggcacggtgg agatcagtgt caccagccag acggcgggga tcagtacagt caccgcaacc
     2581 atcaacagca gcagtcagag ccgggatgtg acatttatcg ccgatgtcag gacggcgcag
     2641 atcgcggatc tggtggtcat taaggatggt tcagaggcgg acggttcgac ggcgaacacg
     2701 ctgcgggcga gggtgaccga tgcgttcggt aatacgcttg ccgggcagac ggtgagcgtg
     2761 ttgggaggca acggcgcaac gactgccccg acggtcatca cagggccaga cgggacggtg
     2821 gagatcagtg tcaccagcca gacggcagga atcagtgtgg tcaccgccag catcaacagc
     2881 agcagccaga gccgggatgt gacgtttatc gccgatgtca gaacggcgca gatcgcggat
     2941 ctggtggtca ttaaggacgg ctcagtggct gacggcgcga cggcgaatac gctgcaggtg
     3001 aaggtcactg atgctaacgg taatgcgctt gccgggcaga cggtcagcgt gatggcaggc
     3061 aacggcgcaa cgactgcccc gacggtgacc actcagccgg atggcacggt ggaaatctct
     3121 gtcaccagcc agaccgccgg aaccagtgtg gtcaccgcca gcatcaacaa cagcagccag
     3181 agccagaacg tcacctttgt accgggtgat gcatcgcagc tcacttcaac cgttgaaaca
     3241 aataagtcga actatacggt tggagagaca atcaccatca cggtaacgct ccgggatgcg
     3301 ttcgataatc tggtaaccgg tgcagcttca cagttagccg ctaatggtgt gctgacggtg
     3361 gctggcaccg acccgtcaga aacgggaagc tgggttgaat caggtggcgt gtataccaca
     3421 acccgaatgg cgacgattgc cagtaccaat cagcacgcca acctgcaatt gcagtcgtgg
     3481 agtgatggcg taacgtcaga tcgctatgac atacagtccg gttctccggc gcaagccaca
     3541 tcaactatcg ccacggataa aaacgcatac accgctggcg agaccataac cgtcgccgtg
     3601 acgctgaaag acgcgcacgg taacctggtt gaagggggcg agtccttact gtctggtgat
     3661 aacgtaatag tggaaggagc cgtacgttca ggaggatggt ctgaaaacgc cggtgtttat
     3721 actgctacat ggtcggcgca aatggcggga gactctcacc acgcgacgct gaagttgtct
     3781 gagtggggca gcagtaaaca atcagagagt tattccatcc acagcggtgc tccggtgcag
     3841 gcaaattctg ctatcaggac agataaatcg gcatatatcg ccggggagcc attaaccgtc
     3901 acagtaacgt tacgggatga gtttggtaac cctgctttcg ggctgacatc agaagtcatc
     3961 gagtcttata ttgatagttt cgcggtaggc ggcgcaactc ccgattctat gcagtgggtc
     4021 gagcagaata atggtgaata taccattgtc tggacagcat ggggtgccga ggaaaatctg
     4081 gttgccagtc tgaaattaaa aacatgggca gcggagatta agtcctcatt atatggaata
     4141 caaccaggtg cagcggcaaa aactcaatca acgattgtcg cggacaaaac gatatatatc
     4201 gctggggata gcataacggt gaccgttgtt cttaaagatg cccagggtaa ctttattact
     4261 gatggtgttg ttcagcttaa tgaggaaaac gtacaagtca ggaatgcgga tcctatccag
     4321 ggcaataatt gggtttacaa cggcaatggg caatatcaaa gacagtatat ggcacatttt
     4381 gcggaagcga atttgaatgc gcaactgaag atggctggtt ggagcgatgc caattattca
     4441 aacaattata ctatcaaacc aggtgaagtg agtccgcttg gttcacagct tcgtatacga
     4501 gaagttttag tagtggaagg ggcggattta cctgtcagtg ctttattagt tgatgatttt
     4561 ggaaatccgg tggataacgg tctggatttg ctggatgatg cagtgtactt acaaaatgta
     4621 gaaaagaaag aaggggaaaa atggagatat gtgggtgatg gcatatatga acgtacatat
     4681 atggcctacc aagaaggaga aaatttaact tcatttatgg agattaaagg ttggcgtata
     4741 tacgggcaac catcctataa catccttcct tttgtggaag tcgagtcgct gagtgttaat
     4801 ggtgtaaaat tcagggcgac agatggtttt ccagaaacag ggtttgatgg tgcaaaattc
     4861 acgctgttac taacccataa tatgaaaaat acggattata attggacggc tgggatttat
     4921 ggaattaatg ttgatagtaa tggagaagta acattatctg tgctgataag aagtgaagtt
     4981 actattactg ggaaacccaa gaatggtaaa gggaatgatg ttgtatttaa attcaagatt
     5041 aaaaaatggt ttactagttt aggtgctagc agtagtaata cctgggatat cataaatact
     5101 tcatgtagct atggtcaaat gccgtcatct ttagaattag cgcagcggcc aagcggtggg
     5161 gtagtaccgc gaaaagtagg tacgttgtgg ggcgaatatg gtaatttaaa aacgtatggg
     5221 aacgctttta gcagtacgga ttattggaca agtactcagc tcatgggagt acacgagaaa
     5281 tttaatcctg aaactggtat atcagagctt gggacaggaa agtcttctgg cttgtgcgta
     5341 gagtattatt aacgaagtaa tcactagatt gccctgcttc agcagggcaa tttatattcg
     5401 ttattacgca gaagtacgca tcacaccaat agtattaaat tcgtttttca cactgagcaa
     5461 acgggggtca tgaagcaaaa catttgacca taaatcattc tctttaatta attgttgcca
     5521 gcgcttttca tattcttttt taccatacac agtgtaaatt aacggcaaaa taacaccatt
     5581 gattttgttg gtgctaatat ttgccagtaa cttcataatg tcatttttta tagatgcgcc
     5641 atagatcaga aacttcgtat aaagcgagtt aacacaggtg taatcaagac catgttcagg
     5701 ctcaagttta agcaataaac ttttggcttt atcattatgt cctttcagtg ccatgaacat
     5761 agccagaata gaggtaataa ttggattgtt acaggtgttt tgactgttaa gatttaatgc
     5821 aaaagatatc gcgttatcga ggggggatgt gtaataaagt ataattaatt taagaatgct
     5881 tatacccatt ttattgggtt caagagcaat gcttttttct attagattaa atgctcttgc
     5941 aaggtcaccg ttcaaaaaac atagcagaga ctgataataa taaacgtctg gcgaattagg
     6001 tttgagtaaa tgtgcttgct taaatagcac attagacact gaatgttcat cttttagccc
     6061 actgattaaa cccagtaaac ccaatgcctg agaatttgaa gggttaattt caagagcctt
     6121 ttcaatggag gttactgccg tagttattgc ttgtttttgt tcactgagtc caaggagggc
     6181 taatgatata tggcattccg ccaggcaaca gtagggcaag gtattttgcg gttgcgtact
     6241 tacacaatct ctaaatatta ctaatgctcg tttcaaactc tctggtgtat aacggtacat
     6301 ttccattctg ccattcatat acataacagc agaatccaga gatggcattt gttgtgtatt
     6361 aatagattta aaattaatgt tgggtatttt ttcaataaga atattaatta cttgtgataa
     6421 taatacggaa agaggattat cgggattgaa atcaatgctt tgatgatcaa tcaatttatg
     6481 tgttctggca tgaattaact caataaataa acgccagttt ttaccatagt gaacgacctg
     6541 acccataata taatattctg gacctgtttt attgataaac tgatggatag tggagaaatc
     6601 actggcctca tttgttaccg aagcgggtaa aatatctaaa ccacaaaaag catattttga
     6661 aaggccctgg acaagttcgt aatggagttt tagaacattg atcgaacctt cggtacgaaa
     6721 gggaaaaacg gcaagagtgg tggtcgactt taccggttca ttatcggtca caatcacaat
     6781 aggtacagta aaacggtatc cacgaccata gaccgtttcg atatacttac actgtttatt
     6841 ctcatgcaaa agtttgcgca atgcataaat acatcgtgtt agagattcat cactggcaac
     6901 actcgcgctc cagaccttat cgataatctc ttctttgcta acaatttcac cgtcagcatt
     6961 aagtaataga atgataacgc caagttcttt cggtg
//
LOCUS       DAYPHN010000206         6993 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000206 DAYPHN010000000
VERSION     DAYPHN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.206"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(35..2818)
                     /gene="pqqL"
                     /locus_tag="ACG0BW_004343"
     CDS             complement(35..2818)
                     /gene="pqqL"
                     /locus_tag="ACG0BW_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFK7261225.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIIKDKENRV
                     NGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARANNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRKLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLAMRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2875..5247)
                     /gene="yddB"
                     /locus_tag="ACG0BW_004344"
     CDS             complement(2875..5247)
                     /gene="yddB"
                     /locus_tag="ACG0BW_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFK7261226.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5285..6970)
                     /gene="yddA"
                     /locus_tag="ACG0BW_004345"
     CDS             complement(5285..6970)
                     /gene="yddA"
                     /locus_tag="ACG0BW_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFK7261227.1"
                     /translation="MITIPITFRMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKDNWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIAVARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPSSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1802 a         1628 c         1569 g         1994 t
ORIGIN      
        1 atcccccgcc aacgaggtgt tgacggggct gttattattt tggcaataat actccggtat
       61 aggtattcac cggatgagaa agatattgtt taacggcagt gttaacattc tctaccgtca
      121 tttgtttcaa caattgctcc tgctcagtcc atgctgcagg atcgtcatat tgaataagac
      181 tatttacaat ggtgttcgct aattgttgaa cgctacgctg ttggatatcg aggctgcgct
      241 gaacgttttg ctggtattcg ttcagttctt gctcactgat ccctttagcc agacgcttaa
      301 ccatcacttc attcgctaac gttaacagtt catcatgtcg ttccggttga caagtaaaag
      361 ccagcaaatg actgatatct ttggcctgag gatcaaccga gaggcgagaa gaaacgctgt
      421 atgccccaga ggcctgttca cgaatattaa tgcgtagatc ttttgccagt gcgacgttaa
      481 aagcatcgag cgccatacgc atcgccagat taaccggtgt ccgggaatca taacgcttcc
      541 actgtgaaac ctgtgccaca ggttcatttt gttcttttac agtaaccgat gcgttgtccg
      601 tcgcgcgagt taatggttta cctgcggcta atggcgaatc agagtgtttg attgatccta
      661 agtaacgcgt aattaacgcc acgagtttgt cttctgcgac attaccgaca atgacaaacg
      721 tgatatccgc tggagatgaa aacaacttgc gatcggcagc cagcgcatct gcggcagtaa
      781 actgtgcaat ctgattttct tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat
      841 acatctgctg ggcgaatttc tccgctggac gctggtcgag tgttttcaac gcctgaattt
      901 gagcattttg tagcgatgcc caaatattat cgttaatcgt gctgtgggtg attcgctggt
      961 taatcaactg aaaaccaggt tcagggttat tagcccgcgc gctaacggag agcaacgtat
     1021 tcatgccact gactttactg ctcatggtta ccgagttttc cgcactccag cgtttcaggc
     1081 tggaagagga gagttcacca acgccgcttc cgcttactgc tttatttgcc agcgcgataa
     1141 gtgatttttg ctgcgcaggg aaacttaaat cgcctttatt cgatacggca gtaatttgta
     1201 gcttttgctc ttcacccgcg gattttgcca gaataaccct ggcaccattg gaaagtgtta
     1261 atgatgtcag attctcagcc agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt
     1321 ctactgttaa cgataaattt ctgcctggga agatgtaagc cgccagcttt ttgttggcgt
     1381 actctttttc cagcgccagg atagccgcag gagacaatgc ttttttggca gcaacctcat
     1441 tgtttaccat ttgctcccaa aatgcatcct ggttctttct taactgctgc catttttccg
     1501 ccagactttg cacggtaatt tgctgccaca gacgtttcga aagttgatat gtctcttccg
     1561 gcgacaagaa cggcgtatta tttaatgagc tggatgccag gcgactggtc agcatacgta
     1621 aatcacgttc agcttgctga tcaaccgcat ttttcagcca ggtgaggcgg gtagatttga
     1681 catcatcgag ttcttcagca gaaaaaccat gctgatcaat agttgccaac tctgccatta
     1741 atgcattcgc agcatcctgc atattatcgt ctcgtgcatt tacacggaaa aacagcgact
     1801 gataatcggg tgcaatttta acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc
     1861 ccgactgtat gcgttcctgc agacgctgat tgaacagctg aaccaacatg ctccattcag
     1921 cttgttcgat aaagctttgc tcatcgctca cttgcaccat tggcaggcga taatagagtg
     1981 cgatgccgtt cacccggttt tctttatcct taatgatatt aaagcgcagg tggttttcgg
     2041 cttttgtcgg ccagacgcga ttttcagctg ctttgttagc cggaagctta cttaaattgt
     2101 cctttatcag cgccagcgct tctttactgt cgatatcgcc gaccacgata aaggtcatat
     2161 tatttggttg ataccagcgt tgataaaatt ggcgcaattg tgccggtgtg accgtagcga
     2221 cagtatccat caggccgata ggttcacggt ctaaattacg ggtattagcc agcaggaaag
     2281 ggcggcgcgc ctgagaggtg cgccatttcg catcctgatg ggcacgccat tcctcagtaa
     2341 ttacgccacg ttcagcgtct acttcgagtt tttcaaaggt tgcggcatta ctccattcac
     2401 tgaagattgc catcacttgt tgcagatttt gtttctgcgt agtcggcaaa ctcacctgat
     2461 acaccgtttc gtcatagctg gtataggcat taacatcgcg accaaaacgc aggcccattg
     2521 actcaaatgt ttcgatgact ttattacccg gccatgtttt tgtgccgtta aacatcatat
     2581 gttctacaaa atgagccacg ccgcgctcat tgtcctcttc ctgcaatgaa ccggtatgaa
     2641 tttgcagcca taaatttacc tgatcctttg gttgagcatg cggataaatc atatatcgca
     2701 agccattgtc cagttgcccg gtaattaact tttcatcctg cggtaaggcg gcggcaatca
     2761 gtcgcccggg gagcaacaga gttgccacta acgtcagtaa gaaacagagg tttctcataa
     2821 ttatctccat gcgaaaaccg ggcaaattta cccggttaag taaaatccga actattaaaa
     2881 tttcatgctg acatcaagcc agaaagtacg gccactggcg taggtcgcca caccggtatt
     2941 cgttgtatca accgcggttt tgctatccag tacattgaga atatcggcac tgatcgtcag
     3001 gttttgttgt ttcagaaatt gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct
     3061 atccagtttt tcgtcaacat actgcttgta atctgaatat tcgctgatgt attgcgcatt
     3121 cgtcttaccc aggataatgc gagctttacg cgcttcttgc caggtcagcg tattcgccca
     3181 caccaacccg ctcggttgat gcgtgaaatc catgtttaag gagatcttta gtggattatt
     3241 aaaatccgcc actggaacgc tatcgtaaga gaccagatta ccgttataaa ccacccggtt
     3301 atcaccggta ttgctctcct cataaccatt gtttaacgac aagttgccct tgctcttgat
     3361 atagctaaag acaatttgcg ggttaatatc tacctggctg atatgcaggg gttcggccag
     3421 ttcaaaactg aggttgaacg aatgcgtttt ggttttgccg tcgttgttat attcagtaat
     3481 ggtggtttta gtcgcgctgt cggtacgact gcttttactg atttgatcat gcgcttcacg
     3541 ataaacatag tttgcacgcg caataacgtt cttaccgatt ttctgctgca accccattgc
     3601 cagttcatcg ttataaggcg ttttcaaatc ctgataacgc gtcagggttt tattgcctga
     3661 taccgattcc gtccagctat tgcggatatc acgtaatccc atatcaagaa tattcccgcc
     3721 atagtaacgg ttataacctg cggtaatcat tgaggtttga tcagcaaaaa tatcccattc
     3781 cgtcataaat cgcggggaga tattgtggtt tgacagatag ttgtcatagt cataccgcac
     3841 accgggcatt aacgacacat tccgccagct aatgcgatcc gccatataca gcgtgtagtt
     3901 gtcaattccc agactgcctt tacctttatg gtaaatggta tggttagttt tctttcctgc
     3961 agcattaatc acataggatt cagactggtt atggcgttca gtccaagcat cggaatagat
     4021 gtattccgcg ccgaagtagg gttgatgcga aacattaccc acggcgaatt tttgccagtc
     4081 caggcgtgtt ttgaaggtgt aattatctac agcctgggaa atgtgtccta atccgccacg
     4141 ggtgcaacgc cctgaaatat caccatatgt acatgaaagt tcggtgtacc agatgtcatg
     4201 atcgtgacgg gtataatcac taatatgatc ccaaccaacg gtggtacgca gtttggccca
     4261 tgcgagctgg gtatccatat cccatgccag accataggat ttattaccca tttcgcgatc
     4321 agactccggg aaggtgctgg tattataatc acggctggag ccggtatatt ttaaggttaa
     4381 atcgtgggta aagcggtcgc tggcaaacca ggtaaatttg ctcaatgcgg tatcgataac
     4441 atttttatac tgtgcccgac cggcgacaat gccgtcattc gaaacataat ccgcgcgggt
     4501 gatatcagac tggcggcgcg ataaaccggc agtaacgcca aagttatcag ccagttcctg
     4561 attaaacgac aaggtataaa agttcttttt aaaatctgga gagaaataag tacttcctga
     4621 agaaccttga ttaaatgcgc tcttgttatt ctcatcgata tgcgatgtta accagtccga
     4681 acgcgtagtg cggtaaccca gtttcacgct gctatcatca gcgttgaagc gtttgatctt
     4741 tgcatcaatt accccgccat tgaagcgacc aaattcaacc ggcacaaaac tgtcataaag
     4801 cgtcacattg tccagtaagc tgacatcaag ataatacccc tgtgacatcc cgctaatatt
     4861 ggttgcacta ctggcatcgg actcattcgc tgggttcagg ttattagttg cactaatacc
     4921 atcaatcaaa taggcattct ggtagggcga cgcaccgtga atagagattt tttccgggcg
     4981 aatatctccc tggttcaacg aggtactttg cgttgaatcc atgcgcaccg cagggtttgt
     5041 tctcagcaga tcgctgatat tgccattccc ggtcggtaaa cgctcgatgc tttcactggt
     5101 gtaatgcgtg ttgccgttta cgggtacagg gacaggagca tagaccgtca tctcttcaaa
     5161 aacatgcatg tacatgtttt tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat
     5221 gacgccagga ataagaactc gcttcattta tttttatctc cgtacagcag ggcttttatt
     5281 gtttttataa aaccgcgcta atgtcacaaa tatcatcggc caggttccag acaccgggtt
     5341 gatgtgtaac cataatgacg ccgcttgagg gtagtttttc acgcactaaa cgcagtaagc
     5401 ggatagcctc ttgttcctca agatgagagg tagtttcgtc aagaaatatc catttcggac
     5461 gtcgtaaaat taatcgtgcc acggcgatac gttgtttttc gccgctggaa agaatatctc
     5521 cccagcgatc gtggtcatga atacgcgcag ccaatttccc cagaccaacc tgatgcagta
     5581 cttcgctcaa cgatttatcg tctacgggca ggggaagtgc tttacaaatt atctctttca
     5641 gtaagccggt tttgattaac ggtgtttgtg acacatacca actgtcagca ggagacgaaa
     5701 tatcaccttt aaaccacggc cagcagtggg ataatgtttt aagcagtgtg gtttttcccg
     5761 caccagagta acctttcagt aatagccatt tgcctggcga aacatgaaag tttaggttct
     5821 ctaatatgat cttattatca ggagtacgaa tactcgcgtc agccacttgt accgcatgtt
     5881 ggcaattttt aggcttattc gtagggcgct gttcggtgag ttgatggaac tcatacaagc
     5941 gatcgataac cgcagccagt tcagcaagtt catcatattt ataaataaac cagcttaaat
     6001 tgttcgatac cagcataaat gcctggcgcg atttcatcag tccgcccaga ttgatctgtc
     6061 cgctaataaa ctgcggcaat aacagaaagt agggaagaac gctaagcgag cgcgaataaa
     6121 tattctgcca gtaatcaagc caccgctgac gattcattaa acgatgccag ttatctttaa
     6181 tggtatgaaa attgtcgctc aactcctggc gttgtagaga ctcagcattg cttagcgcaa
     6241 tcagttcagc ctgcttatta tgctgcacaa gattagttcg aaatgtcgct tcgctacgtt
     6301 gtttttccac attaagcgga cgaatacgtt tacccacctt atgagtaaat aaagttccac
     6361 cgatcacaat gagcacgacg gtatagacca tatacccctg gatattccat tctgttccac
     6421 caacagtgaa tgagagcgta cccgcgcttt gccaaagaat aacggtaaag gtgatcaaca
     6481 tgctaagtga ctggatgaag ccaaatgaaa gactgagcgt tttgctgatc agtaagagaa
     6541 tgtcttcagc gatacgttgg tcagggttat ctgtattttt atgctcaccg tagatctgcg
     6601 tgaagtagta gtttttatct gcaaaccagc gattaaggta gtaatctgtt aaccattcgc
     6661 gccagcgaat agtcagtagt ttaattaacc atgttttgtt tacggatatt aatacaaaga
     6721 tccccagcag ggcagggaac caaaggacaa gctgccaaag cttatcggtt tctttctggc
     6781 ttagcgcatt gaaaaaatca ttattccagt cgtttaacca gacctgaatt ttaaccacgc
     6841 caaggatcat ggcgaggata ataatgatta acagcactga agttttatta ttcttacgca
     6901 gccaaaaggg cttcagcaga cagagatact ttgctatcaa catacgaaac gtaatgggaa
     6961 tggttatcat tagcgaaaat tgattgtgcc atc
//
LOCUS       DAYPHN010000207         6939 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000207 DAYPHN010000000
VERSION     DAYPHN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.207"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="ACG0BW_004346"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="ACG0BW_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFK7261228.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="ACG0BW_004347"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="ACG0BW_004347"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFK7261229.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="ACG0BW_004348"
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="ACG0BW_004348"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFK7261230.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="ACG0BW_004349"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="ACG0BW_004349"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFK7261231.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="ACG0BW_004350"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="ACG0BW_004350"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFK7261232.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPNEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACG0BW_004351"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACG0BW_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFK7261233.1"
                     /translation="MSYSNILVAVAVTPESLQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDETFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1755 a         1627 c         1806 g         1751 t
ORIGIN      
        1 taatgagcac agtgataatt aatggcgaaa tgaatgacgc gcattttcca catctttgag
       61 ttgtggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc gacaatttgt ctgacaaatt gactttccct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag taaaaaggta
      361 atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggtttcc tggtgaagca
      541 accggaagag ccgtggtttc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 cagcctggct gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg
      721 ctctgccatc gtcgcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac
      841 taaaattggc gaacgtcagg gtcaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggcgattac cgccaacgaa ctggatacgg cacgtcgccg
      961 tacaacgggt tctatggatg cgctgaaagc tgcaggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtacttgg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gtatcggtat
     1201 taacggtgtg gatgcggtga gcgaactgtc taaggcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt
     1321 agcaaaagac gttgaaccgc caaaatttac cgaagttacc gacgtggtat tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgattgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggtgtg gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaagcgc tggcgcgtaa
     1981 cgccaaaatt atcgcctttg atgagccaac cagctccctc tctgcccgtg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcacc gtctttaaag atgggcgtta
     2161 tgtcaaaacc tttactgata tgcagcaggt tgaccacgac gccctggtgc aggcgatggt
     2221 cgggcgcgac atcggcgata tctacggctg gcaaccgcgt agttatggcg aggagcgcct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggagaaatt gtcggtctgt ttggtctggt cggggcaggg cgtagcgaat taatgaaagg
     2401 catgtttggc ggtacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ctattaccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcctg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 caaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatta tgaatctctc
     2701 aggtggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggtgct aagcacgaaa tatataacgt
     2821 gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc gaattgtggt gatgcgggaa ggcgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg ttctggcgca
     3061 cctaagtcgt cattcagctt tgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtccttt ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 acggcggtgg ttatcaacct gactgaaagc ctgtggattg gcgtggcagc gggtttgttg
     3361 ctgggcgttc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacga cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc caactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgctgctg
     3601 aataaaacca cctttggtcg taataccctg gcgattggcg ggaacgaaga ggccgcgcgt
     3661 ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc tggcctggta
     3721 tcagcgatag ccggaattat tctcgcttca cgtatgacca gtgggcagcc aatgacgtcg
     3781 attggttatg agctgattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcgcaatacg tggttcgcgg cttaatcctg
     3961 ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttccacaac aatttaacgt tttttcccac cacagccagc cgccacaacg gttggctgtt
     4081 cttcgttaca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaac acaataagaa aagagaagga ggagaaccgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatccgcga aatatgcctg gttttttgat ctcgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aggtcgtcgt gcctgacaat attctgcaag gacttcagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct
     4381 tgacgcactg gcaaaacctt atcgcttccc attagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacgc atatcgttca tctgccggat gcgattgcac gtgatattag
     4501 cgtgcaactg catacggcta tcgcccagtt tcccggtgcg gagctggaga cgaaagggat
     4561 ggcctttgcg ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg cgaggcacca gtaaaggtga ggcgattgct gcctttatgc aggaagctcc
     4741 ctttatcggg cgaacgccgg tatttctggg cgatgattta accgatgaat ctggcttcgc
     4801 tgtcgttaac cggctgggcg gaatgtcagt aaaaattggc acaggcgcaa ctcaggcatc
     4861 atggcgactg gcgggcgtac cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagat gagcacgccg ccagtgccgg tggccttgcc gttggcattc
     5041 tgggggcact gaaagccgca ggtggactgt ggtttggctg gagtggcgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaagg gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcctgctt
     5221 ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa
     5521 acgatcgtct ggcgttcctg gattgccttt ctaacctgac ccgcgtcacg acgcgtagcg
     5581 caaaaagcca tacggcgtgg ggcaaagcgt ttcgaacgga agtttacccg atcggcattg
     5641 aaccgaatga aatagcgaaa caggctgccg gaccactgcc gccaaaactg gcgcaactta
     5701 aagcggaact gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag
     5761 gtttgccgga gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg
     5821 gtaaaattcg ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagctg gacgaattaa tggtaaatac gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ttggtagcaa
     6061 aagagtatgt tgccgctcag gacccagcca acccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa cccctacgat cgtgacgaag
     6181 ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagagatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta
     6361 cctttccaaa gcttgcgtag gagcggttaa tctcccgtaa gtggaactaa cagcacatcg
     6421 acctcactag aggcaataac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatatttgc gacaaacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc tcgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg tgtttcttcc tgcatcacgc tgcgtaaatc ttcaagcatc
     6661 ggcgcagcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgcagact ttccggtgta
     6781 accgcaacgg cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac tcagtcaaac
     6901 aggtaaatat tagtctgatt attagtaaag acaattaat
//
LOCUS       DAYPHN010000208         6927 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000208 DAYPHN010000000
VERSION     DAYPHN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.208"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..341
                     /locus_tag="ACG0BW_004352"
                     /pseudo
     CDS             1..341
                     /locus_tag="ACG0BW_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            427..1449
                     /gene="istA"
                     /locus_tag="ACG0BW_004353"
     CDS             427..1449
                     /gene="istA"
                     /locus_tag="ACG0BW_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFK7261234.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1446..2228
                     /gene="istB"
                     /locus_tag="ACG0BW_004354"
     CDS             1446..2228
                     /gene="istB"
                     /locus_tag="ACG0BW_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFK7261235.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            2296..2904
                     /locus_tag="ACG0BW_004355"
                     /pseudo
     CDS             2296..2904
                     /locus_tag="ACG0BW_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2967..3758)
                     /locus_tag="ACG0BW_004356"
     CDS             complement(2967..3758)
                     /locus_tag="ACG0BW_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFK7261236.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            complement(3765..5735)
                     /locus_tag="ACG0BW_004357"
     CDS             complement(3765..5735)
                     /locus_tag="ACG0BW_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFK7261237.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            6017..6441
                     /locus_tag="ACG0BW_004358"
                     /pseudo
     CDS             6017..6441
                     /locus_tag="ACG0BW_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1756 a         1706 c         1725 g         1740 t
ORIGIN      
        1 gccagtcaga aatatacggc cggcaaggtg tggagctgag ccgttcactg ctgtcgggct
       61 gggtggatgc atgctgccgg ctgctgtctc cgctggaaga ggcgcttcat ggctatgtgc
      121 tgactgacgg taagctccat gctgatgaca cgcctgtccc ggtgctgttg ccaggcaata
      181 agaaaacgaa gaccgggcgg ttatggacct acgttcgtga cgaccgtaac gccgggtcaa
      241 cgctggcgcc ggcggtgtgg ttcgcttaca gcccggacag aaaaggcatc catccgcaga
      301 cccatcttgc ggggttcagt ggtgtactgc aggcggatgc tgtcacgaac ggtgcaatag
      361 tgctccacac ccaacgcctg aaatcagatc cagggggtaa tctgctctcc tgattcagga
      421 gagtttatgg tcacttttga gacagttatg gaaattaaaa tcctgcacaa gcagggaatg
      481 agtagccggg cgattgccag agaactgggg atctcccgca ataccgttaa acgttatttg
      541 caggcaaaat ctgagccgcc aaaatatacg ccgcgacctg ctgttgcttc actcctggat
      601 gaataccggg attatattcg tcaacgcatc gccgatgctc atccttacaa aatcccggca
      661 acggtaatcg ctcgcgagat cagagaccag ggatatcgtg gcggaatgac cattctcagg
      721 ggattcattc gttctctctc ggttcctcag gagcaggagc ctgccgttcg gttcgaaact
      781 gaacccggac gacagatgca ggttgactgg ggcactatgc gtaatggccg ctcaccgctt
      841 cacgtgttcg ttgctgttct cggatacagc cgaatgttgt acatcgaatt cactgacaat
      901 atgcgttatg acacgctgga aacctgccat cgtaatgcgt tccgcttctt tggtggtgtg
      961 ccgcgcgaag tgttgtatga caatatgaaa actgtggttc tgcaacgtga cgcatatcag
     1021 accggtcagc accggttcca tccttcgctg tggcagttcg gcaaggagat gggcttctct
     1081 ccccgactgt gtcgcccctt cagggcacag actaaaggta aggtggaacg gatggtgcag
     1141 tacacccgta acagttttta catcccacta atgactcgcc tgcgcccgat ggggatcact
     1201 gtcgatgttg aaacagccaa ccgccacggt ctgcgctggc tgcacgatgt cgctaaccaa
     1261 cgaaagcatg aaacaatcca ggcacgtccc tgcgatcgct ggctcgaaga gcagcagtcc
     1321 atgctggcac tgcctccgga gaaaaaagag tatgacgtgc atcttgatga aaatctggtg
     1381 aacttcgaca aacaccccct gcatcatcca ctctccatct acgactcatt ctgcagagga
     1441 gtggcgtgat gatggaactg caacatcaac gactgatggt gctcgccggg cagttgcaac
     1501 tggaaagcct tataagcgca gcgcctgcgc tgtcacaaca ggcagtagac caggaatgga
     1561 gttatatgga cttcctggag catctgcttc atgaagaaaa actggcacgt catcaacgta
     1621 aacaggcgat gtatacccga atggcagcct tcccggcggt gaaaacgttc gaagagtatg
     1681 acttcacatt cgccaccgga gcaccgcaga agcaactcca gtcgttacgc tcactcagct
     1741 tcatagaacg taatgaaaat atcgtattac tggggccatc aggtgtgggg aaaacccatc
     1801 tggcaatagc gatgggctat gaagcagtcc gtgcaggtat caaagttcgc ttcacaacag
     1861 cagcagatct gttacttcag ttatctacgg cacaacgtca gggccgttat aaaacgacgc
     1921 ttcagcgtgg agtaatggcc ccccgcctgc tcatcattga tgaaataggc tatctgccgt
     1981 tcagtcagga agaagcaaag ctgttcttcc aggtcatcgc taaacgttac gaaaagagcg
     2041 caatgatcct gacatccaat ctgccgttcg ggcagtggga tcaaacgttc gccggtgatg
     2101 cagcactgac ctcagcgatg ctggaccgta tcttacacca ctcacatgtc gttcaaatca
     2161 aaggagaaag ctatcgactc agacagaaac gaaaggccgg ggttatagca gaagctaatc
     2221 ctgagtaaaa cggtggatca atattgggcc gttggtggag atataagtgg atcacttttc
     2281 atccgtcgtt gacaggatgc atacgccggg ttcaacgagc tgtaccggga tggccggata
     2341 acggaagccg cctgttgggc tcacgcccgc cgtaaaatcc acgatgtgca cgttcgcacc
     2401 ccgtcagccc tgacggagga agcgctgaaa cggatcggcg aactgtacgc catcgaggca
     2461 gagataaggg gaatgacggc ggagcaacgc cttgccgaac gtcagttgaa aacgaaaccg
     2521 ctgctgaaat ccctggaaag ctggctgcgt gaaaagatga aaaccctgtc gcgacactca
     2581 gaactggcga aagcgttcgc atacgccctg aaccagtggc cggcgctgac gtactatgca
     2641 gatgatggct gggctgaggc ggacaataac atcgctgaaa atgcgttgcg gatggtcagt
     2701 ctgggccgca aaaactacct gttcttcggt tcggatcatg gaggagagcg gggagcgctg
     2761 ctgtacagcc tgatcgggac gtgcaaactg aacggagtgg agccagaaag ctacctccgc
     2821 tatgtccttg acgtcatagc cgactggccg ataaaccggg tcggcgaatt gctcccctgg
     2881 cgcgtagcac tgccgactga ataacacatc cccgtcaata cggttcttgc tgcacgctta
     2941 caatttcagg gcagggaata gtattgttaa tttcgggaga aacttaaagt tgccacatcg
     3001 acagtttcat cacacaggct tttgtcctga aaacttttct tattttggac tttcgccagc
     3061 cactgtccaa tatgaagaga aggtactgtg acgataccat cctcattagt tacaaaggtg
     3121 agttctgctt tatgaacagg atgtttgtgc tcaactgcat gagtatgacg ggatgatttc
     3181 agataactga ccgagtcaag ctccatgata gctccggcga gcggggacgt tttatacagg
     3241 actttcagct tcacatcttc actgaaacga cttatatcgg ataacggaat gatttcaagc
     3301 tcatgccccg ttggagactg atagagttgt gctggcatct gctgttcacc aggaataaaa
     3361 gatttcgcat atttagtgac aaactcacag taagccacat catttctgcc ctcccggctg
     3421 accggtttcc atcctgaaga ggttctcgac cagtatcccg gtttcatata tgttgacaaa
     3481 acgtagccat tatctttgct tgcagactca gttttataga cataatccgt ggacgctggc
     3541 ttcagtgtta ttgtctcttt cccgttataa atccggggat tttcaaagaa attccttctg
     3601 tcaggaattg tgccctttga ggggaaatta tgaccataac caatctctgc ggtaatgttg
     3661 ttctctgcct gtttacctgt aatccagaca tcgtgggcca ggacatgccc cgatgctgtc
     3721 agacaaagag ctaagatata acctgtattt ttcatcatga tttgttacca ctgataacgg
     3781 gtataaagac tgacagttct gcctgaaagc ggcactcctt tgccattcat ggcaagcttg
     3841 ttgaaggcat tatccagagt cactgatgcc tcaaagcctt taacacttac aggcttataa
     3901 gacagtgaaa aggaatgtac ggcatagcca gcctgatggg gatcgccttt tttcacatgc
     3961 gtggctgctg ccgaaagttc agcacgccat ccggcagaaa ctcctccatg accaacaggt
     4021 atacttatgt cactgatgag tgtgtcagga ttgccggagc ccagccattc cctgctgctg
     4081 gcatccatac tttccgtgcg gttataagaa agcatccagt caaaataatc agactggtaa
     4141 tttccctgca tatcccatcc ccagatacgg gcccgggata cattataaga ataagaacgc
     4201 atccgtttca tatccacgcg cgtggcaata tagtttctgg catccgttcc gaaatatccg
     4261 cctttcaact gcagccgatc attgcctgta agcaggccgt ccagttgaat acctgcgcca
     4321 atctcacggg taatgttatt ttctggtttc agattaaggt taggtaccca gtaattgggt
     4381 ttacccttgc ggtaaaaatg tacatcatcc ctgtacattt ctgccattgt gggggcacgg
     4441 aatgcagagg atatggagcc gtacatcatc agccagtctg tcggtgagac tgaaatcgcc
     4501 gcccttgggg acaggcgttc ggctttcagt tctccggcac ggggattgaa gctcttgtac
     4561 cggtcatagc gggaaccgac gataatgtta accgggtagc ttttcattgt catttcatcc
     4621 tggaaataca acgatgtaaa gtcaatgttt ccttccggat atagtgtggc agagccttct
     4681 ggcttctgct gctgacggta atattcagcg ccataactta actgatgggc tcccggaaaa
     4741 atcagagtat gactcctgtt gacaacatta acaccggagg ttctgttgtt tctccattct
     4801 gccgttttgt tgtgaccgtt tgtcttgata gtgatatcac tgagatataa agttgacttt
     4861 acattgatga ggctgttatc cacaggagcg tactggtacc agaactgtac gtcattttgt
     4921 actgtttttc tgtcacgcag gccgctgtca ccatgtgtct gagtgggatt gcccggttca
     4981 gtggtgttat tccggtagag acgcaagctg gcacctgctt tgttactgtc tgttataccg
     5041 acagagcctt tcgcaaacag ggctgcaggt ttttccttgt taggtgcgcg ctcaccatca
     5101 ctttgataga tattacctct tttacgcata atgacagaaa ggagcgcatc tgtttttccg
     5161 gttttaccga accaggtgag ccccgagcct gtgctgtggt caccactggc gatatttccc
     5221 cacagactta aaccgtttgt ctctccgggg ggaaggaaat ctgctgcatc ggcagttctg
     5281 aagtccacaa cacctcccag cccgccactg ccgtacagac tggagtttgg tccgcggaca
     5341 acttcgatac gtttgacgag tgccggatcc agataagtgc cactgctttt tgccatgtca
     5401 ctgagttgac ggatgccgtc aacaagaaca agtaccccgc ttttgtcata gccgcgtaga
     5461 ttaaaggtct gcccattggt ccgtcctgca ccagtctggc tgagaccggc aacacctttc
     5521 agcatgccgg ctgatgtgac cgcattctga ctccatggcg tgtcattcgt gacgacactg
     5581 accattgacg gcgtttcaaa taccgtgttc tgatttcctg tcgcgacaac ggtgattgta
     5641 tctttagcca tagaagaatg actggtctct gcctgtaacc ctatcgggag tatgattaat
     5701 gtacaaatga cgtttctgat tacgttcata tacatgcgaa aatatatgtt aatattgata
     5761 tgttataaca tatcatttta ttaatgcaac cctgatcacg cagcgttcag tgataaccag
     5821 tgaatagcga ctctaatacc cggtcgctct gcaatgtatc agcgcctgcg accgatgcta
     5881 tggatattga tttttctacc gataaagaac cagcaatacg gaatttagac tggccccctg
     5941 aatctccaga caaccaatat cacttaaata agtgatagtc ttaatactag tttttagact
     6001 agtcattgga gaacagatga ttgatgtctt aggaccggag aaacgcagac ggcgtactac
     6061 acaggaaaag atcgctattg ttcagcagag ctttgaaccg ggaatgacgg tctcccttgt
     6121 tgcccggcaa cacggtgtgg cagccagcca gttatttctc tggcgcaagc aataccagga
     6181 gggaagtctt actgctgtgg ccgcaggaga acaggtcgtt cctgcctctg aacttgctgc
     6241 tgccatgaag cagattaaag aactccagcg cctgctcggc aaaaaaacga tggaaaatga
     6301 actccttaaa gaagccgttg aatatgggcg tgcaaaaaag tggatagcgc acgcgccctt
     6361 attgcccggg gatggggagt aagcttagtc agccgttgtc tccgggtgtc gcgtgcgcag
     6421 ttgcacgtca ttctcagacg aaccgatgac tggaaatata ggggcaaatc cacatcctta
     6481 cttttccgta aggattttag cgggtcaaaa cagcgttcca tgccggactg atgatctaca
     6541 tacttagagt cacataccaa agtaaaacta tggatgtatg caagcctgca tcgtcaggag
     6601 gttaaatttc agacctgcca gttaccgcgt aaatggcttg atatatgact aatttatttg
     6661 cacaggattt ttacgacaac gctctgaacc agcagttgac gggatatata gttgagctca
     6721 ttacagataa agcttttctg tatctctggc ggtggtcata gaccagtaat cgttattggt
     6781 tgttctatat tatcccgtat gacggtacaa cgtttttaac aactggcgct gtcacaacat
     6841 aagccacccc aaaatattat atgagtaaca atagtctata taggagcgta aattacctac
     6901 tatcaaatac ttactccaat agcattt
//
LOCUS       DAYPHN010000209         6809 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000209 DAYPHN010000000
VERSION     DAYPHN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.209"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            8..961
                     /gene="ompT"
                     /locus_tag="ACG0BW_004359"
     CDS             8..961
                     /gene="ompT"
                     /locus_tag="ACG0BW_004359"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFK7261238.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHTDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1148..2632
                     /locus_tag="ACG0BW_004360"
     CDS             1148..2632
                     /locus_tag="ACG0BW_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFK7261239.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETRENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSY
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(2816..3028)
                     /locus_tag="ACG0BW_004361"
                     /pseudo
     CDS             complement(2816..3028)
                     /locus_tag="ACG0BW_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            3178..3846
                     /locus_tag="ACG0BW_004362"
     CDS             3178..3846
                     /locus_tag="ACG0BW_004362"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFK7261240.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(3791..3928)
                     /locus_tag="ACG0BW_004363"
     CDS             complement(3791..3928)
                     /locus_tag="ACG0BW_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFK7261241.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(3901..4485)
                     /locus_tag="ACG0BW_004364"
     CDS             complement(3901..4485)
                     /locus_tag="ACG0BW_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFK7261242.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGGELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(4485..>6809)
                     /locus_tag="ACG0BW_004365"
     CDS             complement(4485..>6809)
                     /locus_tag="ACG0BW_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFK7261243.1"
                     /translation="KTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATR
                     QASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALED
                     ASTTKKGVVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGADIPDKGRFLNN
                     INAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELASRVIITTATRT
                     AGDPMNNCEFNGFVMPGGRTDRGRYAYGMFWQYQNNERAIHSIMMSNKGDDLRSVFYV
                     DGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVILDFKSGRGFY
                     ESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGTKTYLLLTNQGDV
                     YGGWNTLRPFAINNATGELIIGTKLSASLNGNALTATKLQTPRLVSGVEFDGSKDITL
                     TAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQM
                     KAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHSHVDGRRFNTSTFKDTYQY
                     GSTAVGSNSYQVQGAVGLGVGTMANTNSAGAHTHSLSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         1882 a         1629 c         1460 g         1838 t
ORIGIN      
        1 aacttttatg cgggcgaaac ttctgggaat agtcctgaca acccctattg cgatcagctc
       61 ttttgcttct accgagacta tatcgtttac tcctgacaac ataaatgcgg acattagtct
      121 tggaactctg agcggaaaaa caaaagagcg cgtttatcta gctgaagaag gaggccgaaa
      181 agtcagtcaa ctcgactgga aattcaataa cgctgcaatt attaaaggtg caattaattg
      241 ggatttgatg ccccagatat ctattggggc tgcgggctgg actactctcg gcagccgagg
      301 tggcaatatg gtcgatcagg actggatgga ttccagtaac cccggaacct ggacagatga
      361 aagtaggcac cctgatacac aactcaatta tgccaacgaa tttgatctga atatcaaagg
      421 ctggctcctc aacgaaccca attaccgcct gggactcatg gccggatatc aggaaagccg
      481 ttatagcttt acagccagag gtggttccta tatctacagt tctgaggagg gattcagaga
      541 tgatatcggc tccttcccga atggagaaag agcaatcggc tacaaacaac gttttaaaat
      601 gccctacatt ggcttgactg gaagttatcg ttatgaagat tttgagctag gtggcacatt
      661 taaatacagc ggctgggtgg aagcatctga taacgatgag cactatgacc caggaaaaag
      721 aatcacttat cgcagtaaag tcaaagacca aaattactat tctgttgcag tcaatgcagg
      781 ttattacgta acacctaacg caaaagttta tgttgaaggc gcatggaatc gggttacgaa
      841 taaaaaaggt aatacttcac tttatgatca cactgataac acttcagact acagcaaaaa
      901 tggtgcaggc atagaaaact ataacttcat cactactgct ggtcttaagt acacctttta
      961 acgaagttaa ccagattttc tccccggtgg tattgtataa ccccggggaa tttgtttaca
     1021 gttacaagta gacaatatcc actaattttg taagcattta acctatgtca taacgtgtaa
     1081 tctcttaccg caacacaaga cctcaatata ttcaaatctt atctttattc cgatattcag
     1141 agacacaatg cgaaaaataa ttactcattt caaagttgtt ttaacgttac ttctaccagt
     1201 aaccgtatct gcccagcaga tacagtggca atcctgtatg gccagtcaat tcaaccactg
     1261 gtttggtgag gaaaaaccgt ctcctgactt actatgtggt tatttgtctg ttccattaaa
     1321 atatacagac acaggcggag atgcttctta tgaaaaaaaa tcacaagtca aactagcgtt
     1381 gacaaaattg ccggcaaaaa gcaagcataa aggaagtatc ctgataataa gtggtggtcc
     1441 cgggttacca ggcataaatc cttatattaa ctttgactgg ccagtcacaa atcttcgtga
     1501 gtcatgggat attattggat ttgatcctcg aggcgtcgga cagtccactc cgacaataaa
     1561 ctgccggcaa tcagatacag agactcggga aaacataacc gaaaagcaac aagtattaaa
     1621 taaaattaat gcctgtatcc ataataccgg agccgaagtc attcgccata tcggctctaa
     1681 cgaggctgta tacgatattg atcgtattag gcaagccttg ggggataaac aactgacagc
     1741 cgtggcgtat tcgtatggaa ctcaaattgc agccttatat gcagaacgtt ttccctacaa
     1801 cgtaagatct atcgttcttg atggagtcgt cgatatcgat gacctggagg acaacttcac
     1861 atggcaactc aaacaggcac agagttatca ggaaacgttt gatcgctttg catcctggtg
     1921 tgcgcgtaca aaaagttgcc cgctttcttc agacagagat aaggcaataa ctcagttcca
     1981 tgagctatta tcaaaattac atcacaaacc tttattagac agtaagggag aaaatatatc
     2041 ttcagatgaa ctcatatcat taacaacaga ccttctgcta tggcgttcat catggccaac
     2101 ccttgcaact gccatacgcc agttctctca ggggattgtc agtaatgaaa ttgaaactgc
     2161 gctcagtgct ccgatagcct cagaagagtc aagcgatgct tcgggggtaa tcctctgtgt
     2221 agatcagggg gatgagcaat taacaccaga agagcgaaaa tcccgaaaag acgctcttgc
     2281 gaatgccttc ccggctatta actttgacaa tggacgttcc tattcacctg atttttgtga
     2341 attatggcca atacatagcg acctgaacaa aactcgcctg aaaaatactg ttctgccctc
     2401 tggtttactg tttgtagcac acaaatacga cccaacaacg ccctggatta atgcccgtaa
     2461 gatggcagag aaattttcca gcccgttact aacaataaat ggtgatgggc atacattagc
     2521 tctcaccgga gttaatttat gtgtagataa agcagttgta catcacctga tcactccaca
     2581 aaaaatagaa aatatatact gcccaggaaa ttctgaagca gaaatacaat aatttcaaaa
     2641 tattccgcta tttgctccca atgcaaaaca tatattgcat tgggagcata ttcatatttt
     2701 tgttattctt gtatgaaatc gtctcatgta cacggtcctt ttttcgccga aaactgatat
     2761 tactttaatc aaaagttgac gtgccagatt cgaagtaaat ttataaaata tcaaatcaat
     2821 atacattttg caccttcgag atagtaatgg ctattactgt aacatgagtt acaaacagct
     2881 tcatacccag agctactgat gtatcaacac gattcaacaa tacccgatat ttattccatg
     2941 cctccagcaa cgatctttct tcctccgttg cgatttccag atctacagca tcctgcagtg
     3001 gcgcaatata ctcactgaat tcctggatgt agaactgtgt ggtgacggtc ttccagccat
     3061 tcggctcctg ctgtatcgaa gcataccagg ctatttcaat atcgctatgc tgcggcagca
     3121 tttaacccct tgtaattcat cgccataatt gatttaattc acaaataaaa ctataacatg
     3181 gtgaaattaa tgaaaaaaaa cacagatgat ggggctaaaa tttacacacc acttacccta
     3241 aagctttatg actggtgggt tttgggagta tcaaatcggc ttgcatgggg atgtcctaca
     3301 aaggaacacc ttcttccaca ctttctggaa catttaggta acaaccatct ggatattggt
     3361 gttggaactg ggttttacct tactcacgta cctgagagta gtctgatatc tttaatggat
     3421 ttgaacgaag ctagcctgaa cgcggcatct acaagggctg gggaatcaaa aattaaacat
     3481 aaaattagcc atgatgtttt tgaaccttat cccgcggcgt tacatggtca atttgattcc
     3541 atttccatgt tttaccttct tcactgcctg cctggaaata tatctacaaa aagctgtgta
     3601 atacgcaatg cggcgcaggc cttaactgac gatggaactc tatacggagc cacaattctt
     3661 ggcgatggag ttgtgcacaa tagcttcggt caaaaactga tgcgcattta caatcagaaa
     3721 ggcatctttt caaacacaaa agattccgaa gaaggcttaa cacatatact ctcagagcat
     3781 ttcgagaatg ttaaaaccaa ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa
     3841 aaatagcatc caaccgcagc acgttcttgc ttaagacgtg ctgcggcata atcccaatga
     3901 ttactccctg acagggttcg taggccactc aatatcaggt gcagttgatg tatcaacacg
     3961 gttcagcaac acccgatact ttttccaggc ttccagcaat gaggtttctt cctccgttgc
     4021 gatttccaga tctacagcat cctgaagtgg cgcaatatgc tcactggcta cctgcatcag
     4081 gctgtttttt atttcttccg cctctcggat ccggaacagt ttttctgctt ctgcatcttt
     4141 cacccaggct gtgccgttcc acttctgaaa ctccccttcc ggcgataacc aggtaacatt
     4201 ttccggtaac ggaccgagtt cagaaataaa taactcgccc cctgacgcta cgtcataaac
     4261 cgttttaccc cgatggtctt caacgagatg ccacgatgcc tcatcactgt tgaaaacagc
     4321 cacaaagcca gcaggaatat ctggcggtgc aatataggtg ctgtttgctg gcagacctgt
     4381 atgaggcgga atatatgcgt caccttcacc aataaattca ttagttccgg ccagcagatt
     4441 ataaattttt atggtccgtg gttgttcact cattctgaat gccattatgc aagcctcaca
     4501 atatagttaa atgcaatgtt tttgacggtg ttttccgcgt taccagcagc gttaacggtg
     4561 atggtgtgtc catgtgaacc aatcgcaacg gagtgcgtgt gtgcaccaat accgacagta
     4621 tgtgcgtgtg cgcctgcgct tgcagcagtg ccggacagtg agtgggtgtg cgcgccagca
     4681 gagttcgtat tcgccatcgt accaacgccc agaccaaccg ccccttgtac ctggtatgag
     4741 ttacttccga cagctgtaga accatactga taggtgtctt taaaagtgct tgtattaaac
     4801 ctgcgcccat caacgtgcga gtgtgctcca gccgagcttg tagagccgct cagactatgc
     4861 gtatgagccc cggtgttatt cgtggattta gtgccgtaat caaacgacga tgtggttttc
     4921 gtacccaaat ccgtactgga tgcgctggcg ctgtgggtgt gcgatttaat gccgtcctgt
     4981 tcctgagaca atacggcacg accactggcg ggcttgccct taatcgtcca gccacgcata
     5041 tcaggaataa cgcctgacgg ataagccact gcaagtttcg ggtatgcaga tttgtcaaaa
     5101 gtctgcccct gcatcagggc atagccagac ggaacggtat ctgatggcca cgggattggt
     5161 gcgccgactg ggtagctttc tggtggaaga tttttcgagg tataaacttc tgcccagtct
     5221 tcctcaaaac catagccatc tcttgaggaa cggtagaaca gacctccatt tctgtaatgc
     5281 gccttcatct gcagggtccg gcaacttccg actccggtat agaagttaac cagaatatag
     5341 ctgtcgccag agcgggtgac attgtaagcg cctgattcgg cattccaggg aacgccacca
     5401 tccgcatcgg catatgtatc cgttgccctt ctggcaaaag cagccacatg cgcggcggtt
     5461 aaagtaatat ctttggaacc atcaaactca acaccagaaa ccagtcttgg cgtttgcagc
     5521 tttgttgctg ttaatgcatt accgttcaga cttgcggaca gtttggttcc aataatcagt
     5581 tcgccggttg cgttattaat agcaaacggt cttaatgtat tccagccacc ataaacatca
     5641 ccttgattgg taagcagcag gtaagtttta gtgccatcat tacgccataa tgcaccatac
     5701 tccccaccta tcattcgaat ctgattacca ccacgcgcta caatttcgtc tgtggcaaaa
     5761 agttttttgc acgacaagtt atcgttaacg attaacgaat gagactcata aaaaccacgc
     5821 ccactcttaa aatcaaggat aacgtccgcc gcgatacatt cagtcgccgg atttgttgcc
     5881 ccaaacttat aggtcgtatc attaacaacc agatcagcac caggtgcgga tattgacagg
     5941 ccatcttcga taaacgcaaa aacagggaaa gcagcgccat caacatagaa cacagagcgc
     6001 aaatcatcgc ccttattact catcattatt gagtggatgg ctcgttcatt gttttgatat
     6061 tgccagaaca ttccataagc ataacgcccc ctgtcagtcc ggccaccagg cataacaaat
     6121 ccgttaaact cgcagttatt catcggatcg cctgcggttc gcgttgccgt ggtgataatg
     6181 acccttgatg ccagttcgct tactgagcca gcagaacgca taacaacaac agggtaatat
     6241 tttccagatg ttgcacctgc aggagcgtta acccgcacat aacgcatacc gcgcttatca
     6301 gcaaagtctg ttttactgac cgcgttaatg ttgttcagga agcgtccctt atcgggtata
     6361 tcagcgccgt tctggtcttt ctgcagacgt ttctctgcat tgtcataggc tgattttact
     6421 gcctttggcg ttgccgccag cgtttcagac gtactgttgg tcgcactgct gagctgtact
     6481 accccctttt tcgtcgtgct cgcatcctca agcgccacgg cggatgcaat atcctctgcc
     6541 cgttttgccg ctgtctcggc gcgcgttgcc gcggattccg ccgtactttt gctctgtgct
     6601 gccgccgtcg cactgccagc tgcctctgtc gccttcgtgg atgctgtcgt ggcgctgccc
     6661 ttcgctgctg acgcctgtct ggtcgcctca tcttttgaag cagacgcaga tgatgccgat
     6721 gacgccgccg aactggcgga tgatgcggca gccgtttttg aggattctgc gctggtttcc
     6781 gacgctttcg cgttcgtctc ggatgtctt
//
LOCUS       DAYPHN010000210         6738 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000210 DAYPHN010000000
VERSION     DAYPHN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.210"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..546
                     /locus_tag="ACG0BW_004366"
     CDS             <1..546
                     /locus_tag="ACG0BW_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261244.1"
                     /translation="AVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCSIVPDDHK
                     LEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMATTFTGNPYSTNADNYSLS
                     NMDNGTEIPNVSLVIGDQHGTGYALGAEIKQPIVKDSSTGKGKPKQTLNFKAWLVGET
                     DAVTPTPAPFETLTTFQITYL"
     gene            630..3269
                     /locus_tag="ACG0BW_004367"
     CDS             630..3269
                     /locus_tag="ACG0BW_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFK7261245.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNALADDTIQFDGRFLDLKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNQPLPDDYDIYWYATENDPNKSYACLSPELV
                     AQFGLKEDIAKNLQWIRDGQCLNTALLAGTEISGDLGQSALLVSVPQAYLEYTDSEWD
                     PPSRWDDGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNLRGYAPDISGVAHTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALLGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDDDRARTGNDKEMY
                     TVTYNQNFTDARVSVYLNYSHHTYWDRQDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSNQVSLYHRIDDASHYTVSAGTS
                     ENHSSVDGYYSHDGTLAKVDLSANYHEGQYTSAGISLQGGATLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATK
                     SVVQATLTEGAIGYRKFAVISGEKAMAVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSSPVIPHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3289..4038
                     /locus_tag="ACG0BW_004368"
     CDS             3289..4038
                     /locus_tag="ACG0BW_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFK7261246.1"
                     /translation="MTKYLSAKTLTTVLSLLAATVSFSTQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKLPYLAQSWIEDEKGVKISSPLAVLPPVQRIEAMMNGQVKIQGLPDIH
                     KLPADRESMFYFNVREIPPKSNKSNTLQIALQTRIKLFWRPKALEKNTTQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPVL
                     TYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4013..4561
                     /locus_tag="ACG0BW_004369"
     CDS             4013..4561
                     /locus_tag="ACG0BW_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261247.1"
                     /translation="MKSRAAKANDGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKIDGVSYNRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHSILDLTSGSWLPFNFSSGIPALEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4558..5028
                     /locus_tag="ACG0BW_004370"
     CDS             4558..5028
                     /locus_tag="ACG0BW_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261248.1"
                     /translation="MKRISLLVLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMTLSWSGTQSDFDDAAVSSNITGLG
                     IQLKQAGQRFRINTPLVVNETDLPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5025..5549
                     /locus_tag="ACG0BW_004371"
     CDS             5025..5549
                     /locus_tag="ACG0BW_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261249.1"
                     /translation="MKKKCALIFINSLLLLNSGTTTAVDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNIGQSERVPLVIQLKDCQSSTSLSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGIPVPVNNTTGVTLPLNQGNNSLNFNAWLQAKSGRNVTS
                     GDFNATVTATFEYL"
     gene            5536..6399
                     /locus_tag="ACG0BW_004372"
     CDS             5536..6399
                     /locus_tag="ACG0BW_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFK7261250.1"
                     /translation="MSIYNMKKWTLFFASLMLLVLNVVGTPRSYAGDKLMSSSFDSVR
                     IYYAMDTITGIASSIVLNVTVVTPGEVAYGKYSFLARKGDTLPRISWTGSGPAPTLVI
                     TDYDTINNSHCPGIDTDIFRCAYMTLKITVASDNYGCPWIASFYSYTNLSGFGSYTSP
                     TVHNSICPTIPVASYDISWSENYVSHNKALQLQSTGSTITTTLSTYLMENGKLCDGSV
                     FDSRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKELHDIVVKVNTSSRQPIDS
                     TCRFQYVLNEL"
     gene            complement(6540..>6738)
                     /locus_tag="ACG0BW_004373"
     CDS             complement(6540..>6738)
                     /locus_tag="ACG0BW_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFK7261251.1"
                     /translation="CRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPKEY
                     GGDAALLVLIEVDEWLPPELP"
BASE COUNT         1782 a         1594 c         1693 g         1669 t
ORIGIN      
        1 gcagttgcca cggcaatgct catgggggcg ctgacttcag catcagtcgt tgccgcaggg
       61 aacaacggta cagtgagatt ttacggcaca attgaagact ccccgtgctc aattgttcct
      121 gacgatcata aactggaagt ggatatgggc agcatcggta cgggttcttt aaccggcggc
      181 aaaacaacca caccaaaaga tttccagatt cgtttacagg actgtaattt caatacagaa
      241 acgacaatgg ctaccacatt caccggtaat ccgtactcca ctaacgcaga taactattct
      301 ctgtctaaca tggataatgg aactgaaatt ccaaacgtta gcctggttat cggtgaccag
      361 cacggtacag gttatgcact gggagcagag attaagcaac ctatcgtgaa ggacagttca
      421 acgggaaaag gcaagcctaa acaaactctg aactttaaag catggttagt gggtgaaacg
      481 gatgctgtaa caccaacacc ggcaccgttt gaaacgctga cgacgttcca gatcacgtat
      541 ctctaatgta tttcccccat agctgcaaag ttatggggtt tataactacc aaataactaa
      601 attataaaaa atcatggatg gatacgccga tgccagaaca ttcttttctc cgtttacggg
      661 ggctttcgtg gtggattgct ctggcaattt ctggaagtcc atttaatgcc ctggcagacg
      721 ataccattca gtttgatggc cgatttcttg atttaaaagg caataccaaa attgatttgg
      781 gccgtttttc gcaaaaaggc tatgtagaac cgggaaaata taacttacgc gttcatgtaa
      841 ataatcagcc gttacctgac gactacgata tctactggta cgcaactgaa aacgatccga
      901 ataaatcata tgcctgctta tcgccagagc tggttgccca gtttggtctg aaggaagata
      961 ttgcgaagaa tttgcaatgg atccgcgatg gtcagtgtct gaatacggca cttttagcag
     1021 gtacagaaat tagcggcgat ttaggccagt ctgcactcct tgtttctgta ccgcaggctt
     1081 atctggaata taccgacagc gagtgggacc cgccttcgcg ctgggatgac ggtatccctg
     1141 ggcttattgc cgattacagt atcaacgcgc aaacccgaca tgaaaatggg ggtgatgaca
     1201 ctaacgacat cagcggtaat ggtacggtcg gcgttaacgt tgggccgtgg cgcttacgag
     1261 ctgactggca gagcgattat cagcataccc gcagtaacga tgacggcgac actgacgata
     1321 gcggtacgca aaaaaactgg gagtggagtc gttactacgc ctggcgcgcc ttgccgtcat
     1381 taaaagccaa actttcgctg ggcgaagatt atctcaactc cgatattttt gatggcttca
     1441 gttacattgg cggcagcatc agtaccgatg atcaaatgtt accgccaaac ctgcgcggct
     1501 acgcccccga tatttcaggt gttgcacaca ctaccgctaa agtgactgta acccaaatgg
     1561 gccgtgtcat ttacgaaacc caggtgcccg cagggccatt ccgcattcag gatattggcg
     1621 attccgtctc tggcacgctg catgtccgca ttgaagaaca aaacggccag gtacaagaat
     1681 acgacgtcag cacggcgtcc atgccgttcc tgactcgccc cggccaggtg cgttataaag
     1741 tgacaatggg gcgtccacaa aactgggacc atcaggttgc aggtagcttc ttctcgggcg
     1801 gcgaggcatc gtgggggatt gccaatggct ggtcgctcta cggcggtgcg ttagccgatg
     1861 aaaactacca gtcggcggcg ctgggcctcg gtcgtgacct ggcgctgcta ggcgcgttag
     1921 cgtttgatgt cactcactct cgtgttcaac tcgacgataa cagcgtgtat ggcaacaaaa
     1981 cgctggacgg caactcttat cgcgtcagct atgccaaaga ttttgatgaa ctcaacagtc
     2041 gggtgacgtt tgctggatac cgcttctctg aaaaaaacta catgaccatg agcgagtatc
     2101 tggatgcgaa cgacgacgac cgggcgcgca ccggtaacga caaagagatg tatacggtca
     2161 cctataacca gaacttcacg gatgcgcgcg tttcggtcta tctcaactat tcccaccata
     2221 cctactggga tcgccaggat cagaccaact acaacatgat gctttctcat tatttcaatt
     2281 taggaagcct tcgcaacttg agcgtttctc tgaccggtta tcgctacgaa tacgataaaa
     2341 gtgctgacaa gggggtttat ctgtccttaa gtttgccgtg gggcgacaac agcaccatca
     2401 gttacaacgg caactatggc agcggtgccg acagcaatca ggtgagcctg taccaccgca
     2461 ttgatgacgc cagccactac acggtaagcg ccggaaccag cgaaaaccac agcagcgtgg
     2521 atggctacta cagccacgat ggtactctgg caaaagttga tctcagtgcc aactaccatg
     2581 aagggcaata cacctcagca gggatctctt tacagggcgg ggcaacgttg actgctcacg
     2641 gcggggcgct gcatcgtaca caaaacatgg gcggtacacg tctgttaatc gacgctgatg
     2701 gcgttgctaa tgtgccggtt gaaggtaatg gttccgccgt ttataccaac atgttcggca
     2761 aagcggtggt ggcagatgtt aatgattact accgcaacca ggcttatatc gatttgaaca
     2821 aactgccgga aaacgccgaa gccacgaaat cagtggttca ggcaacgctg acggaaggcg
     2881 ctattggcta ccgtaaattt gcggtgatca gtggtgagaa ggcgatggca gtgctgcgct
     2941 tgcaggatgg cagccatccg ccatttgggg cggaagtgaa aaacgataat caacaacaag
     3001 ttggattggt tgatgacgaa ggtaatgttt atctggcagg cgttaaaccg ggtgaacata
     3061 tgaccgtttt ctgggagggt gaatctcact gcgatatcag cctgccagac ccgttaccga
     3121 atgacctgtt caatggcttg ttgttgccat gccagcaaaa ggggggaagt tctcccgtca
     3181 taccgcatga tattcaaccc gtcattcagg agcagacaca acaggtgaca ccaatggaac
     3241 cgccgatgtc tgtttcatcg aaccaataac gtgattaagg aatgatccat gactaaatat
     3301 ctctctgcca aaacattgac aacggtactg tcgttactgg cagcaacggt aagtttttct
     3361 acccaggcgt cagtaacgcc cgatcgtact cgcctgattt ttaatgaaag cgataaatct
     3421 atcagcgtga cgctgcgtaa taacgacccc aaactgcctt atctggcgca aagctggatt
     3481 gaagatgaaa agggcgtcaa gatcagctcg ccgctggcgg tattgccgcc tgtacagcgc
     3541 attgaagcaa tgatgaatgg ccaggttaaa attcagggcc tgccggatat tcataaatta
     3601 cctgccgatc gggaatcgat gttctatttc aacgtgcgcg aaattccgcc gaaatcgaac
     3661 aaatccaaca cgctacaaat cgctcttcag acgcgtatca aacttttctg gcgaccgaaa
     3721 gctctggaaa aaaacaccac gcaaaaccca tggcagcata aagtgacctt aacccgcaac
     3781 ggtcaggaat ttactgtcaa taacccgacg ccgtattacg tcattttcag taatgccagc
     3841 gcacagaaaa atggcaatcc ggcagccggt ttctcgccaa tagtgatgtc gccgaaaacc
     3901 tctgcaccgc tgaatgtgaa gatgggtagc gtgccggtac tgacctatgt caacgactat
     3961 ggcgctcgga tgccgctctt tttcacctgc aacggtaaca gttgccaggt tgatgaagag
     4021 cagagccgca aaggctaatg acgggagagc cagtatgaaa ggatcttgtt ctgtctgggc
     4081 ggtaactggg ttactgatat gttgtgttgg tagcggcttt gcagttgcag cgagtaagtc
     4141 agtaagtatg aatttacggg tgcttgttga tgcccctcct ccttgtacgg taaatggtgc
     4201 ggcagttgag tttggtaatg tattcattaa taagatcgat ggggtgagtt ataaccgtcc
     4261 cattgactac tcgcttgttt gcaacaattt ggcaatggat gacttgcgtt tacagatgca
     4321 ggcgacaacg gtcgtgatta acggtgaaac agttatcagc acaggtattc cagggtttgg
     4381 tattcgggtt caaaaaagta gtgatcactc aatactcgac ttaacgtctg gttcatggtt
     4441 gccgtttaat ttttcctcgg gcatccctgc acttgaagcc gtgccggtga aacagagtgg
     4501 tacaacctta gcggcagcgg aatttaacgc cagcgccaca attgtggtgg attatcaatg
     4561 aaacggatct cacttcttgt gctgtggggc ttttgctcga tggcactctc aaatgtctct
     4621 tttcacggct atttagtgca gccgcctaac tgcactatta gcaacgctca aaccattgaa
     4681 atcacctttc aggatgtgtt tgttgatgac attaatggca gtaattatga gcaaacggtg
     4741 ccgtattcca ttacctgtga tacggcggtt cgcgacccat caatggaaat gaccttgagc
     4801 tggagtggca cacaaagcga ttttgatgat gccgcagtaa gcagcaatat cactggatta
     4861 ggtattcagc ttaaacaggc tgggcagcgt tttaggatca atacgccact cgtggtgaat
     4921 gaaaccgatt taccggtgtt aacggctgtg ccagtaaaaa agagtggtgt tgatctgcct
     4981 gaggcggact ttgaagcctg ggccacgtta caggtggatt atcaatgaag aaaaagtgtg
     5041 cgttgatttt tatcaatagc ctgctgttgc tgaacagcgg caccacaaca gcggtagata
     5101 atctgcactt taccggcaat ttaatcagta aatcgtgtac accagtgatc aacggtagcc
     5161 agttggcaga ggtacatttt cctgccattg ccgccagcga tttaatgaac attgggcagt
     5221 cagagcgagt gccgttggtc attcaattga aggattgtca aagttctaca tcgctgagtg
     5281 tcagagtcac gttaacggga acagaagata gtgaactgcc cggatttctg gcttttgacg
     5341 cctcgtcttc tgcgtccggc gctgggattg gaattgaaac agcggcaggt ataccagtgc
     5401 cagtcaacaa tacaacaggc gtcacgctcc cgctgaatca gggaaataac agcctaaatt
     5461 ttaatgcctg gttacaggcg aagagcgggc gaaatgtaac gtccggtgac tttaacgcca
     5521 ctgtgacggc aacctttgag tatttataac atgaaaaaat ggaccctatt tttcgcgagc
     5581 ctgatgctgc ttgtcctgaa tgttgtcggc acacccagga gctatgctgg ggataaactt
     5641 atgagttcaa gtttcgacag tgtgagaatt tattatgcga tggacacgat aacagggata
     5701 gcttcgtcta ttgttctcaa tgttacggtg gtaacaccag gggaggttgc atatggaaaa
     5761 tatagttttc ttgcgcgtaa gggggatacg cttccgcgta tttcctggac ggggtcgggt
     5821 ccggcaccaa ctctggttat aacggattat gatacgatta ataattcaca ttgccccggt
     5881 attgatacgg atattttccg ttgtgcatat atgaccttaa agatcaccgt tgcatctgat
     5941 aattatggtt gcccgtggat cgcgtccttt tattcatata caaacttatc tggatttgga
     6001 agctacacga gcccgactgt gcataactca atatgcccaa cgataccggt cgcctcctat
     6061 gatatctcct ggagtgaaaa ttatgtcagt cataataaag cgctacagct acagtccacg
     6121 gggtcgacaa ttaccactac gctttcgacc tacctgatgg aaaacggcaa actctgtgac
     6181 ggcagcgttt ttgatagtag aggtgcatac tgccgtgcgg tatcagaatt gttaaccttt
     6241 acctcttatg gctgcgacaa atcaacggta acggtgaccc ccacacgtca tccggtaacc
     6301 gataaagagt tacatgatat cgtggtgaag gtgaatacca gcagtcggca accgattgat
     6361 tctacgtgtc gttttcagta tgtattgaat gagttgtagc tcgtcgttgt gcactgatgc
     6421 ctgatgtgac gccgacgcgt cttatcaggc ctacgaaatg aatcacaatt tattgaatta
     6481 tcatattttt atcggccgga taaggcattc acgccgcatc cggcattcga tgccagcatt
     6541 cacggtaatt ccggcggcaa ccactcatcc acttcaatca acaccagcaa tgccgcatca
     6601 ccaccgtact cttttggtgc ctggtgaaat gccatcacat gcggatgttg cgccagccac
     6661 agcggcgttt gctgcttcaa aatatgcttc ccatgaccat gcatcacgca ggcacaaaac
     6721 acatgttcgc ggcgacag
//
LOCUS       DAYPHN010000211         6666 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000211 DAYPHN010000000
VERSION     DAYPHN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.211"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(39..743)
                     /locus_tag="ACG0BW_004374"
     CDS             complement(39..743)
                     /locus_tag="ACG0BW_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFK7261252.1"
                     /translation="MVEEQKYPDFAKRLNELMTNKGISVTQLKSLVGVTYEMARRYTI
                     GAAKPRASVMNKLALALGVSASYLEYGVGEREGCKEMASIPNPTKPDVYRIEVLDLSV
                     SAGPGTYMLSDYVDVLYAIEFTTEHARSLFGNRSQDDIKVMTVNGDSMSPTLVSGDRL
                     FVDISVRNFQTDGVYSFVYGKTFHVKRLQMQGNKLAVLSDNPAYEKWYIDEKSQDQLY
                     VMGKALIHESIKYNRL"
     gene            850..1113
                     /locus_tag="ACG0BW_004375"
     CDS             850..1113
                     /locus_tag="ACG0BW_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7261253.1"
                     /translation="MSSQNYTEKAVKAAGKSLSEVARHFGFKSTQSVANWVINNQVPS
                     ERVLQLCELGNWSVTPHELRPDIYPNPNDGLPECYSKVSGSAA"
     gene            1142..1693
                     /gene="ymfL"
                     /locus_tag="ACG0BW_004376"
     CDS             1142..1693
                     /gene="ymfL"
                     /locus_tag="ACG0BW_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFK7261254.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            1869..2048
                     /locus_tag="ACG0BW_004377"
     CDS             1869..2048
                     /locus_tag="ACG0BW_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFK7261255.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            2038..2979
                     /locus_tag="ACG0BW_004378"
     CDS             2038..2979
                     /locus_tag="ACG0BW_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7261256.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRRARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWIAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            2976..3470
                     /locus_tag="ACG0BW_004379"
     CDS             2976..3470
                     /locus_tag="ACG0BW_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFK7261257.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRERCLRKSSKRAVSGEEWYLSGN
                     YVGA"
     gene            3470..3796
                     /locus_tag="ACG0BW_004380"
     CDS             3470..3796
                     /locus_tag="ACG0BW_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFK7261258.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEVAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            3793..4182
                     /locus_tag="ACG0BW_004381"
     CDS             3793..4182
                     /locus_tag="ACG0BW_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFK7261259.1"
                     /translation="MKLILPFPPSVNTYWRHPNKVAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            4202..4999
                     /locus_tag="ACG0BW_004382"
     CDS             4202..4999
                     /locus_tag="ACG0BW_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFK7261260.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            5007..5996
                     /locus_tag="ACG0BW_004383"
     CDS             5007..5996
                     /locus_tag="ACG0BW_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFK7261261.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPEHYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLDFNDNHAV
                     TMPELCWWLVRNDLADALPESAARKALRLPKSVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEQYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            6014..6397
                     /gene="quuD"
                     /locus_tag="ACG0BW_004384"
     CDS             6014..6397
                     /gene="quuD"
                     /locus_tag="ACG0BW_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFK7261262.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
BASE COUNT         1700 a         1533 c         1806 g         1627 t
ORIGIN      
        1 tattttttca cctttagttc tatagtctaa acgaagaact aaagccggtt gtatttaatt
       61 gattcgtgta tcaatgcttt gcccataaca taaagttgat cttgggactt ctcatcaatg
      121 taccatttct cataggctgg attatccgaa agaacggcga gtttgttgcc ttgcatttgt
      181 agacgtttaa cgtggaacgt cttaccataa acgaaagagt aaacaccatc agtttggaag
      241 ttgcgaacag aaatgtcgac aaacagtcga tctccggaaa ctagagtagg ggacatgctg
      301 tcgccattta cagtcataac tttaatatca tcttgagaac gattaccgaa aagagaacgg
      361 gcatgctctg ttgtgaactc aatggcgtag agcacatcaa catagtctga aagcatatag
      421 gttccaggtc ctgcgctaac gctaagatcc aaaacttcta tcctgtatac atcgggtttt
      481 gttggattgg ggatgcttgc catttcctta catccttctc tctcgccaac accatattct
      541 aaatatgaag ctgatacccc cagagccaat gcaagtttat tcatgacaga ggcacgaggc
      601 ttcgcagcgc cgattgtgta tcgccgcgcc atttcatatg taacgcccac aagacttttg
      661 agttgggtga cagaaattcc cttgtttgtc attagctcgt ttagtctctt ggcgaaatct
      721 ggatacttct gttcttctac cataggtaga aggttactcg catcacatac gctagtcatt
      781 tctattttaa gtagttgcat tctgctattt taagtagcat catccctctg aatttcagag
      841 gagaaaggta tgtcatctca aaactacaca gagaaagcag taaaagctgc gggaaaatct
      901 ttatctgaag tagcccgtca ctttggtttt aagtccactc aatccgtcgc taattgggta
      961 attaacaatc aagtcccgtc agaacgggtt ttacaacttt gtgagttggg aaactggtcc
     1021 gtgacccctc atgaactgcg tcctgatatt taccccaatc caaatgatgg attacctgag
     1081 tgctattcaa aagttagcgg ttcagctgcg taaacgtaac cacagaaacg aggagttaac
     1141 cgtgggtaag catcactgga aaatagaaaa acagcctgag tggtacgtga aagctgtcag
     1201 aaaaactatc gcagcgttgc cggggggtta cgctgaagct gctgactggc tggacgtaac
     1261 agagaacgca ttatttaacc gccttcgtgc cgatggcgat cagattttcc cgctgggatg
     1321 ggcaatggtt ttacagcgtg ctggtggcac tcacttcatt gctgatgctg tggcgcagtc
     1381 tgcaaatggc gtatttgtgt ctcttcctga cgtcgaggat gtggacaacg ccgatattaa
     1441 ccagcgtctg ctggaagtca ttgaacagat cggcagttat tcaaaacaga ttcgttcagc
     1501 aatcgaagac ggtgtagtgg aaccgcatga gaagacagca attaacgacg agctgtatct
     1561 ctcaatttcg aagctgcagg agcatgcagc actggtctac aaaatctttt gcatttcaga
     1621 aagtaatgac gcccgcgagt gtgcagctcc gggcgccgtg gcgtgtcgtg actgtggaga
     1681 aactaacgca tgaacagttt aacaacacac taccgtcgct cgcaactgat tgcgcttcct
     1741 gtaccgggtg gaaaagcgaa ggtggaatat tgctatgcag tgaatgtacc aggtgacagg
     1801 gaaattgtaa cccacagctt tgctgagtgg gctgtgggtg atttcaaccg gcagaaggag
     1861 acagtccttt gcgacaagtt aaccgctggt tcaaagatca ctacggagtg cccgtcagag
     1921 tcattcgttg ggaaccggaa acacaacgtg ttatctacct ccgcgaaggc tatgagcatg
     1981 agtgcttcag cccgctcgaa cagtttcgtc gtaaattcag ggaaatagag gtcggtcatg
     2041 agcactaaat taaccggcta tgtatgggat ggttgcgctg catcaggcat gaaattatcc
     2101 agcgtggcaa ttatggcccg cctggctgat ttcagtaatg acgaaggtgt gtgctggcca
     2161 tcaattgaaa ccattgcccg ccagattggc gcggggatga gtaccgtcag aacggctatc
     2221 gcacggctgg aagcagaagg ctggttaacg cgtagggcgc gtcgccaggg taaccgcaat
     2281 gcgtcgaatg tttatcagct taacgttgcg aagcttcagg cagcggcatt ttctcaactg
     2341 tcagattctg acccgtcaaa atctgacgca tcaaaatctg acccgtcaaa atttgatgcg
     2401 tcgaaatctg gcaaaaaagc gggttttcac ccgtcagaat ctggtgggga tccgtcagta
     2461 aaatcaaaac atgatccgtc agataaaaaa acttctcgtc cggacgcttc gcaaccggac
     2521 acgcagacgg ctgaacagga ttttttaact cgccatcctg atgcggttgt attcagccct
     2581 aaaaagcgcc agtgggggac gcaggatgat ttgacctgcg cacagtggct ctggaaaaaa
     2641 atcatcgccc tgtacgagca tgccgccgaa tgtgacggcg aggtggttcg tcccaaagaa
     2701 ccgaactgga tagcctgggc aaacgaaatt cgcctgatgt gtgtgcagga tggtcgtact
     2761 cataaacaaa tctgcgagat gtacagccgc gtcagtcgcg atccgttctg gtgccgtaac
     2821 gtgctcagcc cgtcgaagct gcgggaaaaa tgggatgagc tttccctgcg cttatcgccg
     2881 tccgtcagca cgtacaccga aaaacgcgaa gacccgtact tcaaagccag ttacgacaac
     2941 gtggactaca gccagatccc ggcaggattc agggggtgat catgagtctt ttgaatgacg
     3001 ttcagaaatt cattgaagcc catccggggt gtacttccgg agacattgcg gatgcttttg
     3061 caggttactc acgacagcgc gttctgcagt cagcaagcaa gttacgtcag agtgggcgtg
     3121 tggctcaccg ttgtgaaggg gatacacgca gacatttccc gcggctgact gagataccgc
     3181 aggagccgga accgcaacca gttcgtgaat ccagacctgt gcgcaatttc tatgtcggca
     3241 ctaacgatcc ccgggtgatt ttgtgcctga cccgccaggc ggaagaactg gagtccaggg
     3301 gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc cactcccagc
     3361 aagagcgaaa caattttctt gcgcgtcgtg agcggtgttt acggaaaagc agcaagcgcg
     3421 ctgtatcggg tgaagagtgg tatctgtcag ggaattacgt gggggcttaa tgacgacgtt
     3481 aactcaatgc cagcagcagg tgctggatat gctgatttct taccagaaag aacgtggctt
     3541 cccgccaacc aatcaggagg tggctaccat gctgggatac cgttcagtga atgcagcggt
     3601 ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg gcgtggcccg
     3661 gggcatcacg cttcataccg cggtgaagga cgacgacagc gaggtggccg ggattatccg
     3721 cgcactgctt gctggtgagg aaaacgccag gctgcgtgca gcccactggt tacatgagag
     3781 ggggctgaaa gtatgaagct gattctgcct tttccaccca gcgtgaacac ctactggcga
     3841 caccccaaca aagtggcatt tgctggtaag agcctgataa gcgcggcggg gcgaaaattc
     3901 cagagtgcgg cgtgcacagc aatagttgag cagttacgtc gtctgccgaa accaacgtcg
     3961 gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg cgatctggac
     4021 aactataaca aggcgctgtt tgacgccctg acccacgcgg gtgtgtggga agacgacagc
     4081 caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cggaagggaa ggtcgagatc
     4141 actatcagta agtacgagaa aacggcgggt gcagccgcct gatcaagagg agaaacgaag
     4201 tatgaataat ctgatggtca ttgatggtat tgaagttcgt cgtgatgctt atgggcgtta
     4261 cagcctgaac gatctgcaca gggctgccgg ttctctggat aagcataagc ctgcattctg
     4321 gctccgcaat gagcaaactg aacgtttaat aagcgagttg cagatttgca actcggtcaa
     4381 tatagagcca gttaacgtta ttcgtggcgg aaataaccag gggacgtatg tctgcaaaga
     4441 actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg tgatccgtac
     4501 tttcgatatg gtaaccagcg catcggaaaa attatccggg caggctgctg acaagatgca
     4561 ggctggcgtg attctgctgg actttatgcg ccgggagtta aacctgtcta actcttcagt
     4621 gcttggtgcc tgtcagaaac tccaggaggc tgttggctta ccgaatctgg caccgcgcta
     4681 tgccattgat gctcctgctg acgcgcctga tggctcaagc cgtcccacgc tgtcactgag
     4741 tgcactgctg aagcagtatg gtatccgcct gacagctaat caggcatatc accagatggt
     4801 gaagctgggg atcgtcgagc agcgcgaacg atacagccgt accgcgatta acaacatcaa
     4861 aaaattctgg tcgctgacag cgaaaggctg catgttcggc aagaacatca ccagtcccgc
     4921 aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc tgttaaagct
     4981 gctcgatacc gttcattgag gtgaccgtga gagcactatt gacccctgaa attgccccgc
     5041 gtatggggat cgtattgttc aggcccggtt cagagctgat gcccctgttt atgcaggggc
     5101 gtgtactgct ggagcctgag ccggagcatt attcatcttt cgccagtggt gccgttcccg
     5161 cggcatcaca accgctggcg gatgatcctg ccgttcgggc cgtgttccgt aatgaggcag
     5221 tgattcgtcg tgctggtggg gtggaatgtc ttgaaagctg gttacttcgt gaaaaaggct
     5281 gccagtggcc tcattccgac tggcacagcg agaacatgac cacaatgcga cacgcgccgg
     5341 gcgcaatccg tctgtgctgg cactgcgata atcagttacg tgatcagttc acggagcggc
     5401 tggaatcaat ggcaacggat aactgtgccc gttgggtgtt gtctgtagtc cgtcgggatc
     5461 tcgattttaa tgataaccat gccgtgacaa tgccggaact gtgctggtgg ctggttcgta
     5521 atgacctggc ggatgcctta ccggaaagcg cagcccgtaa ggcactgaga ttaccgaagt
     5581 ctgttttgcc gtctgtcacc cgggaaagtg accttgtgcc ttcggttcct gccaccagca
     5641 ttatccagga taaagcgaaa aaggtgctgg cgctgaaagt ggatccggag tcgccggagt
     5701 cttttatgtt acgccccaaa cgtcgtcgct gggttaatga acagtacacg cgctgggtta
     5761 agacgcagcc gtgtgcatgt tgtggaaagc cagctgatga tccgcatcat ctgataggcc
     5821 atggtcaggg tggaatgggt acaaaagcgc atgacctttt tgtgctgcct ttgtgcagaa
     5881 aacaccacga cgaactgcat gcggataccg tggcatttga agagaagtat ggctcccagc
     5941 tggagctgat atttcgtttt atcgatcgcg cactggcgat tggtgtgctg gcctgatttt
     6001 gtggagaaag ttaatgcgtg atattcagat ggttcttgaa cgttggggag cgtgggcggc
     6061 taataatcat gaagatgtga cctggtcgtc cattgccgcc ggttttaagg gattaattcc
     6121 ttcaaaagta aaatctcgcc cgcaatgttg tgacgatgac gcgatgatca tttgcgggtg
     6181 catggcccgt ctgaaaaaga acaacagcga tttacacgat ttattagtag attattatgt
     6241 atgtggtatg acattcatgt cactggcaag taagcattgc tgctcggatg gttatatcgg
     6301 gaaaaggtta cagaaggctg agggcataat tgaagggatg ttaatggcat tagatatccg
     6361 gttagatatg gatatcgttg ctaataattc taattgatat gcaattgttt actaaaagtt
     6421 attaaaaatg gggcgtggaa acgcccccaa aataaagggt aatatataac agaaggttta
     6481 tatagtaaga agcaaggtag tgcttctaaa ggaagtggct tgagggctcc acttatatgt
     6541 tgcggaggca aagcctcccg caacatatct ttttcgtaag tcagattaga actgataaac
     6601 cagacctaca gcgacgatgt cgtcggtatc aataccagct gttttggtaa acttactatc
     6661 gtcaat
//
LOCUS       DAYPHN010000212         6598 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000212 DAYPHN010000000
VERSION     DAYPHN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.212"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..250
                     /locus_tag="ACG0BW_004385"
     CDS             <1..250
                     /locus_tag="ACG0BW_004385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261263.1"
                     /translation="ASQTNREGGASEVATMMHAADNIEKVRIADLVITINKTEEEEAK
                     GEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            398..1513
                     /locus_tag="ACG0BW_004386"
     CDS             398..1513
                     /locus_tag="ACG0BW_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFK7261264.1"
                     /translation="MSELKELITELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGDATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            1589..2371
                     /locus_tag="ACG0BW_004387"
     CDS             1589..2371
                     /locus_tag="ACG0BW_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261265.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPISLKVQVGAWMDGKVIYTNT
                     VFCASNDSIIEEAIQESLLTMHSLFLLEDKQNYEEYLRSIKGERTLSLKADFVTPTNL
                     LEVLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAGKMVR
                     IANELPMIEQEAKSYATNHVTELLVPYRLESDQRKMISWGSW"
     gene            2427..2909
                     /locus_tag="ACG0BW_004388"
     CDS             2427..2909
                     /locus_tag="ACG0BW_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261266.1"
                     /translation="MAVIVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTLKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEVKQQ
                     "
     gene            2906..4228
                     /locus_tag="ACG0BW_004389"
     CDS             2906..4228
                     /locus_tag="ACG0BW_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFK7261267.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            4228..4404
                     /locus_tag="ACG0BW_004390"
     CDS             4228..4404
                     /locus_tag="ACG0BW_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261268.1"
                     /translation="MLGWMIAFLAVGFFIGIVVMSSCINDYIKSGVIERRGRIYRIVE
                     ITNTLKEIKDDRIK"
     gene            4388..4603
                     /locus_tag="ACG0BW_004391"
     CDS             4388..4603
                     /locus_tag="ACG0BW_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261269.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGVLK"
     gene            4600..4752
                     /locus_tag="ACG0BW_004392"
     CDS             4600..4752
                     /locus_tag="ACG0BW_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261270.1"
                     /translation="MIPYISLAFMGGFLIGFGICRDLIKQELKTKTLCIGKRVYRVVH
                     ETKVKK"
     gene            4749..5039
                     /locus_tag="ACG0BW_004393"
     CDS             4749..5039
                     /locus_tag="ACG0BW_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFK7261271.1"
                     /translation="MSNLTSWEWWLATYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            5818..5892
                     /locus_tag="ACG0BW_004394"
     tRNA            5818..5892
                     /locus_tag="ACG0BW_004394"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5850..5852,aa:Asn,seq:gtt)
     gene            5898..5984
                     /locus_tag="ACG0BW_004395"
     tRNA            5898..5984
                     /locus_tag="ACG0BW_004395"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5932..5934,aa:Tyr,seq:gta)
     gene            5989..6064
                     /locus_tag="ACG0BW_004396"
     tRNA            5989..6064
                     /locus_tag="ACG0BW_004396"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6022..6024,aa:Thr,seq:tgt)
     gene            6282..6368
                     /locus_tag="ACG0BW_004397"
     tRNA            6282..6368
                     /locus_tag="ACG0BW_004397"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6314..6316,aa:Ser,seq:tga)
BASE COUNT         1944 a         1372 c         1612 g         1670 t
ORIGIN      
        1 ggcatcgcag acaaaccgtg aaggtggcgc gtcagaagtt gccacaatga tgcacgctgc
       61 cgacaacatc gaaaaagtac gtattgctga cctggtaata acgatcaaca aaaccgaaga
      121 agaagaagcg aaaggagagg ctcgtctcta ctttgctggt tcacgtaacc agcagggagg
      181 gatcagcatt cgcgttaaac aaaacctcga acaaatgcgc ttcattgagc gaatcttaga
      241 cgttacctaa aaaataagcg tggagaacac ctccacgctt gattcattgg tgaaacaact
      301 tttcttttgc caaaccacaa aagaaaaaca catgagcctt tatgttatat caacatttag
      361 gttggtcaca atattgcctg ttaaaagtgg aattatcgtg agcgagctga aagagctaat
      421 taccgaatta gattttgaac aatggttgga tactgaaggt atcgtttatc gacgtggagg
      481 cgtgagtact cgcggtcgtg aagtgaatat caaggagtgt ccggtatgcg gcagctccaa
      541 ctggaaggta tatttcaatc tgaccagtgg cgtcggtaaa tgcttcgctg gtgatcatcc
      601 cgaagagatt cagttcaata agctggtctt cctcaagcac tacagcggca aatcacgacg
      661 acagttcgag gaatatgtgc agaacgccct tctttcccag gggtgggcac caaagaaaga
      721 agagctagtg cttgcaagca cagtcgagtt agaggggcca gttgcactcc ctcgtcatta
      781 cgagcttcct atagatggtc gtcttccaga ctatctggtt gaacgaaaca tatcgcctga
      841 aatggcaaag tattttgacc tacgatactg cgtcgaaggc aagcacgcct atgtagatcc
      901 gtatacagat caggttaaag gacagatatt cgatatgcga atactgatac cggtttacga
      961 tctggatggg gtaatgaaga catttcaggg acgagacatt accggtacag cagaacgccg
     1021 ctacctcttt cctatgcagc ttccagcttc aggtaaattt ctctacaacg gccataatgc
     1081 agtcggcaaa cagactgtag ttgtctgtga gggtgcgttc gatgttatgg gggtcaaacg
     1141 agctattttc gacgaagaaa cattacgtga ttacgtggag ccaataggaa cgttcgggat
     1201 gcatctatct ggcaacacca ctcaggatgc agaagatcag ttgggcgcgt tcctgacgct
     1261 caaggcgcgt ggattacgta atgtgatcat gatgtgggat agtgaaaagc aagctatacg
     1321 caacacgatg gccgcagcca ggcgactgac cagtattggt cttaatgtca aagtggcgtg
     1381 tttgggcgag gaaggactcg acccgggcga tgcaacgcca gagcaaatca tcaaagccta
     1441 ttatcgggca aaaccgtata ccaagcagtt ggagttgcaa agcaaggttt tgggcattaa
     1501 ggctctatca taacaatcac cttcaaaata agtagatgat tacttatctt tctgtgagaa
     1561 tactttcatc tgttagctag gagttggtat gaaagacgaa attcagaaat tagcctgcga
     1621 catcattgat aaaactggtt tagaaatcag cgagagcaat cggctagaca tcattgaaaa
     1681 agccgtaaaa acagcaatgg atcatatcgc cactcgtttg gtcgagatcc cgctaccggg
     1741 gctaccttat ctgaaggtta agttacacgt atggggtgaa ccttcttgtg cacggcgttc
     1801 tgcattagtt gtttttatta gcaaagaaaa cccgatcagt cttaaagtgc aggttggggc
     1861 atggatggat ggcaaagtga tctacacaaa taccgttttt tgtgcgtcaa atgacagtat
     1921 tattgaagag gctattcaag aatcacttct aactatgcat agcttatttt tgctggaaga
     1981 caagcaaaac tacgaagagt acttacggtc gataaaaggt gagagaacat tgtcacttaa
     2041 agccgatttc gttaccccga caaacctgtt ggaagtcttg cttaataaag gggctaatga
     2101 tgccgtaaat gtaatcagag agagtgagta tgcgtctctt tgcgacatgt gcaaaagcca
     2161 gttggatctg gtgcatatcg ttattgatgc tgggaaggca tgtgatggcg taatggcgga
     2221 gtttgctggg aagatggtca ggattgctaa cgaattaccg atgatagagc aagaggctaa
     2281 atcatacgcc accaatcatg tcacagagct tcttgtcccc tatcgcttag aaagcgatca
     2341 gcgcaagatg attagctggg gaagttggta agctctcagt gtgtcgtttt ttacgcaaat
     2401 aatgataggt aagcacaaga ttatttttgg cggtaattgt gaaagctgat ttgtcaaaaa
     2461 tcccctctat ttcaggaaat aatggttatt cacttcgttg tgaggaagta aagataaacg
     2521 gtgagtcggc atattgcagc tactccgttt gccagcacac cattcttgcc ttcaaagaaa
     2581 accgtcttcc tcgaacttca ttccagtcgt gcgcaaccgc tatcaaagca ggcaaatgca
     2641 aggcgttaaa aatgatggtt gaagagattc gtaaaggaga atctctgtat ttcgaagata
     2701 tgaccgcgct cattaaggag gttgaagaac ggaataaaca agccagaact ttaaaacgaa
     2761 aacgtgacag tgtaacgatt aatagcatgg ttaagaagag caccacatca caaacagcga
     2821 tcactgacgt gtatgcggcg ttgcttgaag aaacaacaaa agaaacacat gagcaaatcg
     2881 atcaacatat ggaggtaaaa caacaatgaa aaagttgatc gcacttaagc ataagctgga
     2941 cgaaatgaaa gctatgggca ccaatgcaaa aaaagaggca ttggccaaca tggatgactt
     3001 tgaacaaagc atggtttcat tgatgctcaa ccctttcatc cgttttgggg taaagaaata
     3061 caaagtggca gagccgctta gtgagtccat cccaagtgac gaaaaagcca ttgatgtact
     3121 gaataagctg gcctctcgcg agctaacggg gaacgcagca atagcagctg ttgagtctat
     3181 cgtggcgtca atgtgcgccg atgggcagga cgtgttccgt cgtttcctct taaaagaccc
     3241 gaaagctggc gttgggatta gcctatgcaa caaggttttt gaaaatccca ttccgaaatt
     3301 cgaggtgcag ctggcgtcac cgtataaaga aaaaggcgac aaatacccct tcaagccaaa
     3361 tcctaaagca aaatggccga tgattggcag tcttaagctc gatggtttgc gagtaatttg
     3421 cgaggttatt gttgacgagg aagaggttaa cttcctttct cgtactggta atccaatcac
     3481 gtctctcgat cacctaaagc cagccatgct cgaattaggc aaactttcag gccacaaaca
     3541 catcttcttc gatggtgaag gaactgccgg ttcatttaac cagtccgtat ctgcattgcg
     3601 caaaaagaac gtgcaggcaa ttggcgctat ttatcatgtt ttcgacttct tcctaccgga
     3661 atggcgagca caggctaaat ccaaagagta tgcaaagaca ggtatgaagc tgaaagagcg
     3721 cctggctatg ctggtggcgt tgttcaaaaa cgatcgcagt gaaggctaca cacaagacat
     3781 tcacctgcat ccgttctata tcatccatag ccacgaagac ttcatcgaac gcttcatgaa
     3841 acgcctggac gataacgaag aaggggagat gggcaaagat ccgaactctg tttacgagtt
     3901 taaacgtacc cgcagctggt ggaagttaaa agacgaagat tcagaagatg gtgaaattat
     3961 cgactttgag ccgggcgacc cggactctgg ttttgccaac acacttggaa aaattgttat
     4021 tcgtcttgaa aacggcgtca tcgttcgtgc gagcggtatt aagcataaat atctggacga
     4081 gatctggaac aacaaagaga agtaccgtgg tcgtattgtt gaggttcatt gtcacgagaa
     4141 aacaccggat ggcagcttac gccacccccg actgaaatgg ccgcgttgct tacgcgatac
     4201 agaagatcga atcggagata aagaatgatg ctcggctgga tgattgcatt tttagcagtt
     4261 gggtttttca taggtattgt ggtgatgtcc agttgcatca atgactacat taaaagcggt
     4321 gttatagaaa gacgcggtcg tatttatcgc attgtagaaa taaccaacac cctgaaggag
     4381 attaaggatg atcgtattaa gtaaacggga gaaggaaacg cttcatgaaa tcagtaagtg
     4441 gccggagttc cctgagtact ggaagcctaa aacgcgagct aagttagagc gtttagggtt
     4501 ggttgcaaac gtttctgaaa cgtggtgttc ggccaactac cagttaactg ataaagggaa
     4561 agtattgcta cagcaattag tagaatcagg agtgttaaaa tgattccata catctcatta
     4621 gcttttatgg gtggcttcct tatcggcttc ggcatctgtc gtgatttaat taagcaggaa
     4681 cttaaaacca aaacactgtg catcggaaag cgtgtgtatc gggtagttca tgaaacaaag
     4741 gtgaaaaaat gagcaattta acttcttggg aatggtggtt ggccacctat ttcttagcgg
     4801 ccggagtcgc attcgccttt tacgtaggtc agttagtcgt aaaactgttg ctgattaaat
     4861 ttgctagtca taaacgtatc gatgatggtc tgtggcgtct tggcaccctg gtggaaactc
     4921 gctacgggca acttaaggag aacgaaacca ttactatcca agcgaaacga ttcactgcca
     4981 ccattacaag aacacctagt cgtagagtgg ccttgatcaa aaaagtcaca accgaataaa
     5041 aacatattga taagtattta cttacttatc ttttgtgtat aagatgactt tgttttcgtt
     5101 gagacgcgac tgtttgaact taaatacaag tgcaaacgaa gaagtctatc tggcagtagc
     5161 ctaataagcc aaacaccagc gaggtcagtt tccagcctcg ttaccgaaat gggacacact
     5221 gagcgagtgt gattgcagaa cgcaggaggg aacattcatg ttccctccga tgaagtaaca
     5281 gaatgggcgg ttggtatatt ttcaactcca tatgactccc ggattcttag ccactgaccg
     5341 cccattctgt tacgtcattt tgttcaatta tgtcgtttat actgggttaa aaagcggcga
     5401 cgtagcccgg ctggtatggt tagccagcac acaacgttga ggccattaca tttttatcaa
     5461 ttctaaggtt ctattcacag agataccggc gagcgttgat atgtaacatg ttgggcaaac
     5521 attcaatcgg agtagtggcc tcaacgttgt gaagacggga ttgttgtgta ggtttaacca
     5581 ctgttgccat tggtgcctgt tttcacaaca aatgattcca tacatcacat tgtataaatt
     5641 acaaagtagg tgctgtcctc agaaacatca tctacttaaa gattttgcct tctactattg
     5701 agcgaagtcg aaagcgtctg gcactaacga aaagtgcaag tagcggtgcg tttcctggca
     5761 gaaactaaac cgtcgcgatt ggcactgttg agtaataaat actggcagtg ctgaattgat
     5821 ggtgtagctc agcggtagag cagttggctg ttaaccaact ggtcggtggt tcgaatccac
     5881 ccaccatcgc caatttaggg gagttagtcc gtagaggtag cggtgtagac tgtaaatcta
     5941 ctgtcatggc gactcgggtg gttcgactcc atcactcccc accaaattgc cggtttagct
     6001 cagttggtag agcgcctgcc ttgtaagcag gatgtcagcg gttcgagtcc gttaaccggc
     6061 accaacacaa caggaaagag cattgaaaac cgccgagact gccctgtttt aagcgtaagt
     6121 ccgtgcaaac gtcatacagt gctcttttcg ttgtggttga tgcgcagact gatgcgcgga
     6181 aagaaatgcc ccagcgacac acgaggcgag ttcacacact gcaatgcagt cagtagccgg
     6241 ggtcgctaag ccggagatca gtaccggtaa ccacaacaat tggagagtag ggagcatggt
     6301 gctcaagcgg tcttgaaaac cgtcccattg tgcaaacgat gatggttcga ttccattact
     6361 ctccgccaga cacagcgttg agcggtttgg ccttttaatc aactcgatta agactccgct
     6421 aacataaacc agaccgctca acgctgtgat agataattac ggcagacgtt cttaaccata
     6481 gcttgctaac atcctagcaa cacttttttc agcgcaaaat tcaaaggggc ttcggcccct
     6541 tttttgttga acagtgcaat ttactttgca tagtcgccca attttaatat ttcgcgct
//
LOCUS       DAYPHN010000213         6560 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000213 DAYPHN010000000
VERSION     DAYPHN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.213"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            111..1295
                     /locus_tag="ACG0BW_004398"
     CDS             111..1295
                     /locus_tag="ACG0BW_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFK7261272.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMAATPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1406..2329
                     /locus_tag="ACG0BW_004399"
     CDS             1406..2329
                     /locus_tag="ACG0BW_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFK7261273.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2333..3151)
                     /gene="nlpA"
                     /locus_tag="ACG0BW_004400"
     CDS             complement(2333..3151)
                     /gene="nlpA"
                     /locus_tag="ACG0BW_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFK7261274.1"
                     /translation="MKLTTHHLRVGASLLLAGILLAGCDQSNSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3361..3654
                     /gene="yicS"
                     /locus_tag="ACG0BW_004401"
     CDS             3361..3654
                     /gene="yicS"
                     /locus_tag="ACG0BW_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFK7261275.1"
                     /translation="MKPTMLLMITVFLTFSAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            3956..4453
                     /locus_tag="ACG0BW_004402"
     CDS             3956..4453
                     /locus_tag="ACG0BW_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HFK7261276.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNTKMATELFNTVVTYSQNEKIKEMATAYLELLTANTEE
                     EVQTE"
     gene            4463..4644
                     /locus_tag="ACG0BW_004403"
                     /pseudo
     CDS             4463..4644
                     /locus_tag="ACG0BW_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4700..6481
                     /gene="sctE"
                     /locus_tag="ACG0BW_004404"
     CDS             4700..6481
                     /gene="sctE"
                     /locus_tag="ACG0BW_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFK7261277.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPEMQVLDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYSSSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNALNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CESIKNQSEVMKEINHVRENKLNETARKYTKWTKVVKIVNECVTVATIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQDTIELVKSIYASAAASIVMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITSTIKSTIESIIKSVSKNIIKVLNRVCSMIQTSVVVLKLIAK
                     ISNALEKIGLAICETATSIMNYCVAENSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTETLKFASHSIV"
BASE COUNT         1887 a         1434 c         1424 g         1815 t
ORIGIN      
        1 cattgctttc tggttgtatc agcgacactc aaaaattctt aatacaatac tcattcgact
       61 ggtacttatt tgtaactcag ttatattttg tcgcccggtg attcagaaga atgcaaaaaa
      121 cggctaccac tccatcaaaa atacttgatc tcactgccgc ggcattttta cttgtcgcct
      181 ttctgacggg tattgcgggc gctctccaga ctcctaccct aagtatattc ctcgcagatg
      241 aactgaaagc ccgtcctata atggtaggtt ttttcttcac cggtagcgct attatgggaa
      301 ttcttgtcag tcaatttctg gcaagacact ccgataaaca aggcgaccgt aaattactga
      361 tcctgctatg ttgcttattt ggagtgctgg cctgcacgct ttttgcgtgg aatcgcaact
      421 acttcattct cctctctacg ggcgtacttc tgagtagttt tgcttccacc gcaaacccgc
      481 aaatgttcgc cctcgcccgt gaacacgccg acagaacagg ccgtgagacg gtaatgttca
      541 gtacattttt acgtgctcag atctcgcttg cctgggttat cgggccaccg ctcgcttatg
      601 agctggcaat gggatttagt tttaaagtga tgtatctcac cgcagccatc gcatttgttg
      661 tttgcgggct gatagtctgg ttgtttttgc catcaataca aagaaatatt cctgtcgtta
      721 cccaacccgt agaaatttta ccctccaccc ataggaagcg ggatacacgg ctacttttcg
      781 tggtttgttc aatgatgtgg gcggcgaata atctctacat gataaatatg ccgctattta
      841 ttattgatga actgcatcta accgataaac tggctggaga aatgattggt attgctgccg
      901 gtctggaaat tccgatgatg ttaatcgcag gctattacat gaaacgtatt ggcaagcggc
      961 tattaatgct cattgctatc gtgagtggaa tgtgttttta cgccagcgta ctcatggcgg
     1021 cgactccggc agttgagctg gaattgcaaa ttctaaatgc catcttcctt ggtattctct
     1081 gtggtatcgg catgctttat tttcaggacc tgatgcctga aaaaataggc tctgcgacaa
     1141 cgttatatgc aaatacttca cgcgtcggct ggattatcgc cggctctgtt gacggaatta
     1201 tggttgaaat ctggagctac cacgcgttgt tctggctggc gatagggatg ttgggtattg
     1261 cgatgatttg cctgctgttt attaaagata tttagctcac ctgtctgggc taagaaaaat
     1321 cggcgtggcg caaacagagc catcctcgcc atttttgcta tcatgcctgc atacataaac
     1381 gacaaaacag tatgcagagg gaaaaatggg ttccaccaga aaggggatgc tgaacgttct
     1441 gattgccgcc gtgttgtggg gaagttcagg ggtctgcgcg caatacatca tggagcaaag
     1501 ccagatgtcg tcgcagtttt tgactatgac gcgtttgata ttcgccggtt tgattctgct
     1561 gacgctgtca tttgtgcatg gcgataaaat cttttctatt attaacaatc acaaagatgc
     1621 cattagtctg ctgatttttt ccgtggttgg tgcgctaacc gtacagctca cttttttact
     1681 aaccattgaa aaatcgaatg ctgccacggc aacggtgctg caattcctct caccgacgat
     1741 tatcgtcgcc tggttctcac tggtgcgtaa atctcgcccg ggcattctgg ttttctgcgc
     1801 tattttgaca tcgctgatcg ggactttttt attggtgaca cacggtaatc cgacgtcatt
     1861 atcgatctct ccggccgcgt tattctgggg cattgcctcg gcatttgctg ctgcattcta
     1921 taccacctat ccctcaacgc tgattgcccg ctatggcacg ttaccggtcg tcggctggag
     1981 tatgctgatc ggtggtctga tcctgctgcc cttctatgcc gggcaaggaa caaacttcgt
     2041 ggttaatggc agcctgattc tggcgttttt ctatctggtg gtaatcggaa cttcgctaac
     2101 ctttagtcta tatctgaaag gcgcacagct cattggcggg ccaaaagcca gcattctgag
     2161 ctgtgcagaa ccattaagta gcgcgctgct ctctttgctg ttgctgggga tcacgttcac
     2221 attgccggac tggctgggaa cgctgctgat tctgtcatcg gtgattttga tttcaatgga
     2281 ttctcgtcgc cgcgccagaa aaataaatcg tccggcgcgg catgagtgag aattaccagc
     2341 caggcaccgc gccaccgtta aagatggttt ctgccgcttt agcgacttcg ggcgattgat
     2401 aagattgcag aaattccttc acgttttctg cattcttatt atcttcccgt gccaccagaa
     2461 tattcacata cggcgaattc ttatcttcaa taaatacgct gtcgtgcacc ggagaaagcc
     2521 cggtctgctg aatgtaagtg gtgctgataa tcgccacatc aactttggga tcatccagta
     2581 cgcgcgggag ctgtgccccc tcgagttcca taatctgcaa atgacgagga ttctcggtta
     2641 tatccagcgc ggtaggtagt aacccttttc cctctttcag agtaattagc ttctcttttt
     2701 gcaacagtaa aagcgcgcga ccaaggttgg tcgggtcgtt ggggatcgcc actgtcgcgc
     2761 cttcttttat ttgcgccacg gttttgattt ttttggaata acccgccatg gggaagacaa
     2821 acgtatttcc caccgctacc agtttataac catgcgcctg attatcttgt tcaagaaagg
     2881 ggcgatgctg gaagacgttc gcatcaagct cgccatgatt tgtcgcatcg ttcggtagca
     2941 acgaaccgct aaatcctacc agttcaacat cgaggccata cttctctttt gccacctttt
     3001 tagcgacttc cgcgacatct tgttctgcgc cattaataac gcccacttta atgtgctttt
     3061 catcgctgtt gctctggtcg caacctgcca gcagaattcc tgccagcaat aatgaggccc
     3121 ccacccgtag atgatgtgtt gtcagtttca cttttttatc ctttttttaa tcgttaactg
     3181 actataatgg caagatcact acgatttaaa aaacgaaaag ctatcgaata gaagaaaaag
     3241 gtatataaaa ctggcacttc ttactgacca cgcaaacacc tttcgtacag catgatgaga
     3301 gcggcaaaag caaactacac ttatgcttca ttcgatgcac atttgcagaa ggttgcagct
     3361 atgaagccaa cgatgctact catgattacc gtattcctga ctttttcagc catttctcag
     3421 gccgaatcgc cattcagttc tctacagtcg gcaaaagaaa agaccacggt attgcaagat
     3481 ttacgtaaaa tttgcacgcc acaggcgtca ttatcagacg aagcgtggga aaaattaatg
     3541 ttgtctgatg agaataacaa acaacatatc cgcgaagcta ttgtggcgat ggagcgtaat
     3601 aatcagaata actactggga agcgctgggt aaggtggaat gcccggatat gtgaatttcg
     3661 cacacctccg cgctcaggcc agcagccgct gacgtacgct cgcacatcag taaatcacct
     3721 gctttttcaa attccaatca aaattgcacc cgttcatcag gattgtggag gaatttccct
     3781 ctacattgtt ttcatttcat cacatagaaa atcttatctc attgaaacaa tacttgtttt
     3841 taatgaagaa aaggaagccc aacggatggg cataaaaatg agcgaatttt gatatcacca
     3901 ccctctaaaa aatggtgaag tctgcacaaa gaatgttcga aaggagagag tagagatgga
     3961 aagcgcaaat ctggatttag aagaaaataa agaaattgcc agtaagttcg aacgtgcact
     4021 aggtatggga gcaacacttg cagagctgca tggcattact ccggatacac ttgaaggcgt
     4081 ttatgcttac gcttataact tttatgaaaa aggaagactt gacgaagctg aactcttttt
     4141 taaatttctc tgtatttatg atttccaaaa ttataactat ttaaaaggat atgccgccgt
     4201 ctgtcagttg aagaaagatt atcaaaaggc attcgacatg tatcatatct gtttaatgct
     4261 atctcccgat aacgatttct ctttggttta ttacatgggc cagtgccaga tgggcttaaa
     4321 aaacactaaa atggccacgg agttatttaa tacagtcgta acctacagcc aaaatgaaaa
     4381 aattaaagag atggctactg cttatcttga actcttgaca gccaataccg aagaggaagt
     4441 tcaaacagag tgagtcggca aaatgaacat aaacggaaaa aactcttctt ttacgcctga
     4501 agagttaacc aggaaatatc atgagaaagc cccggttgat ttaaaaataa ccatagcgat
     4561 aaaataaatg agaagaataa caataaacac attgcttact ttcccgttga tgccatttta
     4621 gatgggaata aacatctgcc atgagttaaa taaaaaagaa ttaattacac ccgatgatag
     4681 cttaaaaagg aggtgaatta tgtcggcacc gattacggga caaacgatca cgtttgaaca
     4741 aatcagcgaa acattgcgca cacagtatag cgatgcggaa aaaagactgc aggacagcag
     4801 caaaacgcaa gttgatccca tgcggcttaa taaaaacccg aaatcccttg ataatgatat
     4861 tcgcgctcgt cttgaaaaca aaccgatgct tgctccgcct gaaatgcagg ttttagattc
     4921 agacaacgcc actacagcaa agacgaacga tgcccgcctg acgatgattt tgggtaatct
     4981 aacaggcatt gccgatcagg atattacaac acgattgcat aataaccttg acagcaccct
     5041 gcttcgacac gaaatggcac ataataagtt tcgtgaatta agtgacgctt actcctcgtc
     5101 attagattcc gcgcaaaaag ccgacgacat catgcatcag gccaataata attacaatgc
     5161 ggtcgataaa aaggtgcaat cgctggagaa aaaagtcaat gcattgaatc aagagctgtc
     5221 gcaactgcaa cctggcgatc cgcaatataa taaagtgctg acgcaaaaaa atgcggctga
     5281 gaaaacactc acgctatcat tacaaaaaaa atcgttagct gagaaatcgt taaatacagc
     5341 cattatggat gctgatgctg cgatcggcca aagtatggaa atttttgacg aaattcaaca
     5401 gcaagaacag attaataact ttaccaccaa tatttgcctg acccaggaaa accagaaaaa
     5461 cagaaacgcg acagccacat ttatcctttt gattacctca gtaatggaag ttattggtga
     5521 taccaattgc gaatctatta aaaatcagtc tgaggtaatg aaagagatta accatgtcag
     5581 ggaaaataaa ctcaatgaaa cagcgcgtaa atatactaaa tggacaaaag ttgtaaaaat
     5641 cgttaatgag tgtgtaactg tagctaccat tgccatgagc gcaatcttaa ttgttgttgg
     5701 tcttcttgct gcagttccaa gtggtggttc aagtattgcg ggtgcattgg cacttattgg
     5761 aggaatagcg ggcgcagtag tattaggtgt agatatcact tgtcaaatcg ctctgggcac
     5821 cacggctacc ggctggattt tagggaaagt tgtagaaggt ctttcggctg ctattaaaac
     5881 agttgatccc actctgctcg caatcaccgc gcttctggac gttattggtg tcgatcaaga
     5941 tacgattgaa ctggttaaaa gtatttacgc aagtgcagcc gcttctattg tcatggcgac
     6001 agtaatgatt ggtgcagctg taatatgttc tgtagcaata ggggccgttg tttctgcatt
     6061 atcaaaaaca gccgcagaag aagtcaccaa agaaataaca agcactataa aatcaaccat
     6121 tgaatctatt attaaatccg tttcaaaaaa tattataaag gtattaaaca gagtatgcag
     6181 catgatacaa acatcagtcg tagttttgaa gttgattgcc aagataagta atgctctgga
     6241 gaaaataggc ttagctatct gtgaaacagc aacatcgata atgaattatt gtgttgctga
     6301 aaactctgcc gacatggcaa ttttacaaca ggacatgagt aatctatcaa aaacgcgtga
     6361 acaaatgctt tcagtattgc aaagggtgga taaaaccgtc gaacaagagg taagtcagat
     6421 ggtaagagta ttacaacacc gaactgaaac cttaaaattt gcttcccatt ctatcgtata
     6481 agagggttct aacatggcca cctatgaaat taaaaatgcg accagcacac ataatattaa
     6541 caatctcgat aaaacaatct
//
LOCUS       DAYPHN010000214         6418 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000214 DAYPHN010000000
VERSION     DAYPHN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.214"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            3..788
                     /locus_tag="ACG0BW_004405"
     CDS             3..788
                     /locus_tag="ACG0BW_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261278.1"
                     /translation="MQSDYGNLNIKNLAFDSFKERLQSTMTALTFFISTGQCACDEIA
                     ESNFNYMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEIRIRGINEN
                     FVGMDFTCEFRPEKMTNLINIKNRLVIHYLKTIYYEQNYIHPVGSIFAAIQKNESLLK
                     FPSVISILNVNLLFNPLNLPGMGSGVLEEIMSISDSSLRKRLGYEVLSFSLQAHSLSQ
                     ECIDKLAIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(792..2183)
                     /gene="tcyP"
                     /locus_tag="ACG0BW_004406"
     CDS             complement(792..2183)
                     /gene="tcyP"
                     /locus_tag="ACG0BW_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFK7261279.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2316..2906)
                     /locus_tag="ACG0BW_004407"
     CDS             complement(2316..2906)
                     /locus_tag="ACG0BW_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFK7261280.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3069..3737)
                     /gene="hxpB"
                     /locus_tag="ACG0BW_004408"
     CDS             complement(3069..3737)
                     /gene="hxpB"
                     /locus_tag="ACG0BW_004408"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFK7261281.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3884..4420
                     /locus_tag="ACG0BW_004409"
     CDS             3884..4420
                     /locus_tag="ACG0BW_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFK7261282.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4461..5321)
                     /locus_tag="ACG0BW_004410"
     CDS             complement(4461..5321)
                     /locus_tag="ACG0BW_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFK7261283.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5427..5717)
                     /gene="ghoS"
                     /locus_tag="ACG0BW_004411"
     CDS             complement(5427..5717)
                     /gene="ghoS"
                     /locus_tag="ACG0BW_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFK7261284.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5818..>6418)
                     /gene="pfkB"
                     /locus_tag="ACG0BW_004412"
     CDS             complement(5818..>6418)
                     /gene="pfkB"
                     /locus_tag="ACG0BW_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFK7261285.1"
                     /translation="AALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLIS
                     AAQKQGIRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEI
                     VNSGKAKRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENA
                     SLEEMVRFGVAAGSAATLNQGTRLCSQDDTQKIYAYLSR"
BASE COUNT         1707 a         1553 c         1545 g         1613 t
ORIGIN      
        1 taatacagag tgattatggt aatttaaata ttaaaaacct tgccttcgac tcttttaaag
       61 aaagactgca gtctaccatg acagcactca ctttctttat atcaacgggt caatgtgcct
      121 gcgatgaaat agcagaatca aacttcaatt atatgattgc ctatatgtct aacattaact
      181 atgatgcctc aaagccagga gcgccagcac ttagttttga tacatatctg caggacaatg
      241 ttaaatatag agtgataatt aataatcttt atggtagtga aattaggata agaggtatta
      301 atgagaattt tgttgggatg gattttactt gtgaattccg accagaaaaa atgaccaatt
      361 tgatcaatat aaaaaacagg ttggttattc actatttaaa aacgatatac tatgaacaaa
      421 attatataca tcctgttggg agcatttttg ctgcgattca aaaaaatgag agtctcctaa
      481 agtttccttc ggtaatatca atacttaatg tcaatcttct ttttaatcca ctgaatcttc
      541 cgggaatggg aagtggtgtg cttgaagaga ttatgagtat ttcagatagt tcacttagaa
      601 aacgtcttgg gtatgaggtt ttaagctttt cgctacaggc acattcattg agtcaggaat
      661 gtatcgataa gttagccatt tttttcgctg acgatctgtt taaatatgag tcagtatgca
      721 tcgccgcgat ggaacattta aaaagtaagg caacggctcc tatacaaaac ggaccgttgc
      781 cagcataaga attaacggtg tgccagttcg gcgtcgtctt cgctatccag aatggcttta
      841 tcggtttgct tcagccactg gctggtcagc gtgccagcgg tcatcgagcc actaacgttt
      901 agcgccgtac gccccatgtc gataagcggt tcaacggaga ttaacagcgc caccagggta
      961 actggcaggc ccatcgcagg cagcacaatc agtgcggcga aagttgcacc accaccgaca
     1021 ccggcaacgc ctgcggaact aacggtaaca ataccgacca gcgtcgcaat ccacatcggg
     1081 tccatcgggt taatgccaac cgtaggcgca accatcaccg ccagcatcgc cggatacaaa
     1141 ccggcacagc cgttctgacc aatggttgca ccgaaagagg cggcgaaact ggcgatggat
     1201 tcaggaacac ccagacgacg cgtttgtgct tccacattca gtgggataga cgcagcactg
     1261 gaacggctgg taaaggcaaa cgtcagtact ggccatacct tacggaagta cttcagcggg
     1321 ctcacgccat taatacccag cagaaggcca tgcactgcaa acataatcag aagaccgagg
     1381 taggacgcga caacgaaact tcccagtttg atgatgtctt gcaggttaga acctgcaacc
     1441 actttggtca tcagcgccag aacgccgtaa ggggtcaact gcatgaccag gcgaaccagc
     1501 ttcattaccc agctttgcag ggtatcgata gcggttaaga cgcgttcacc tttcggcgca
     1561 tcatccttca gcagtttcag cgcagctaca ccgaggaatg cggcaaaaat taccacgctg
     1621 ataattgacg tcggattggc tccggtcaga tcggcaaacg ggtttttcgg gataaaggac
     1681 aagaccagct gcggaacgct caggtcagag actttaccaa catagttact ttcaatggcg
     1741 ttcagacgtg cagtttctgc accaccctga accagacctt cagccgtcaa accaaacagg
     1801 ttggtgacca gcacaccgac cagcgccgca atcagcgtgg taaacaaaag cgtaccgatg
     1861 gtcagaaaac tgattttgcc taactgagat gcgttatgca gacgggcaac cgcgctcaga
     1921 atagaggcga agactaacgg cataacgatc atttgcagca gttgaacata gccgttacca
     1981 acaatgttaa accactgtac agaatcttta agtacctggc tgtcagaacc ataaatggta
     2041 tgcagggcaa ggccaaaaac cacacccatc accagaccca ccagcacttt tttcgccaga
     2101 ctccactgtt tatggcgggt ctgagccagc gcaaacagca gtacaacgaa caccacgatg
     2161 ttcgcaatta atggaaagtt catccccgtt ctcctgatct tattatttct atcactgcgt
     2221 agcagtcata gtgttggcgc aagattaaca gaactgtgag ctggctctta tattcaaatg
     2281 gaattgctta ttccagaaag gtgatttgtg cctttttagt attcaacctg atgcttaata
     2341 agccgatgaa actggatctg caaagtattg atcatttgcg atgaccaacg aacagcgctg
     2401 ttttcgggta atgaatgggg catccatacc gaccagacaa ataatgccat gcagataaaa
     2461 cgttccagtt ggttgccctt ttggggaacc aggataggca agcggaaacg ccagcggcat
     2521 ggccagagca ggggaacacc ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa
     2581 cccagcacca ttccttgtag cgcatcagcc ggaatgaacc agctttcagg aaccttaagg
     2641 taaaaggttg ccagcagcgc aaataccgcc agcagactgt gggtaaaacc acgatgccca
     2701 aaagcgcggg cgatcggttt tgatatccac tttaatcgct gcccaagaaa cgactttggg
     2761 tgatcgatgt ccggtaacaa acacgtcagg attgcggaag ggacaatatg ccaccagtca
     2821 ccctgtgcca gcacgggcgt cagctcggca tttttggcaa ataccgcaca agcaatagaa
     2881 aagagaaggt gaccttccgc cgtcatgata aaactcacta aactgtcgat acgtacagta
     2941 taggattttt atacagtgcg cggaagtggt gacggtgtaa ctctttctct taaattgacg
     3001 attaatccgt cagttgaatg caaattcaga taaagaacca ggggcagcgt gatcactgcc
     3061 cctggtcatt aaccgagaag gtcttttgcg gtgagttctg tcagtgatga gagtttgacg
     3121 tttgctaata caaaacgtgg atcattttgc gcttctggcg caggaacgac gatggaacgc
     3181 atgcgggctg ctttagaggc gatcatgcca tttactgaat cttccagcgc tacgcaggtc
     3241 agtgggtcaa cgcccagttt tgctgcgcag tcgagatata cttgcggatg cggcttgctg
     3301 taaggcagtt tttcagccga ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc
     3361 aacacttttt ccagcatatg tagtggtgac gcggaggcca gtcccaccaa taaaccttgt
     3421 tctttgcata acgcaacggc ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc
     3481 agtgaaatgg cacgggcaat aacccgttct actacttcct gacggcttgg cccattccac
     3541 ggttgccggg cgtaccaaag atcgaccacc atatcgatac gtaaacctaa ggtgtccggc
     3601 agctcgttac gacgggagat atccaccccc aggcttgcca tcacatccag ttcggctcga
     3661 tcccataaag gttctgagtc gataagtaat ccatccatat caaaaattgc agcaagaatc
     3721 tgacgcgggg ttgacattac aacctctcct ttagagcatt atgtgtaagg tattaagccg
     3781 tgcttatgat taagcgccta tcatatcgcc tttaaagaat gggcgaaaac agatatctac
     3841 aggtaaacta agtgaaacga cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc
     3901 tggacgcatt gctgttttga aaaggatttt gggttgggtg atttttatac ctgcattgat
     3961 ctctacactg atttctttgc tgaaatttat gaatacccgg caggaaaacc aggaaggcat
     4021 taatgcggtc atgctcgact ttactcatgt catgatcgat atgatgcagg cgaatacgcc
     4081 ttttttgaat ctgttctggt ataactcccc gacgcccaat tttaatggtg gcgtgaacgt
     4141 tatgttctgg gtgattttca tcctgatttt tgtcggactg gcgttgcagg attccggtgc
     4201 ccggatgagt cgccaggcgc gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga
     4261 aaaagccaaa ggggaagagg ggctaacgcg tgaacaaatt gagtcacgta ttgttgtgcc
     4321 gcaccatact attttcctgc agtttttctc gctgtatatc ctgccggtta tttgtattgc
     4381 tgcaggttat gtgttctttt ctctgctggg gtttatttaa tcaaaaggcc gcacggagcg
     4441 gccattatcc tcaacccgta tcatgctgct aataatctat ccagtgactg ctgagcaata
     4501 accaaatgct gaccgccaaa taaccttgca cgatttaaca gcgtgtagag ttggtaaacc
     4561 ggttgacgtt caaggaaatc ggcaggtagc ggggatactg actgatagcc gtcatagatt
     4621 tgtggcggct gttcagtatg cagcggtaac atcgccaggt cgcactctcg gtcaccccag
     4681 tagcaggccg ggtcgaaaat gtacgggcca tccggaccca gcgcacagtt gccggaccat
     4741 aaatcgccgt gcaacagaga aggctgcggt tgatgcgagg ccagacgctg ctgaatatgc
     4801 tcgacgatgg catcgatatt gccgaaagcg atcccttttt ctgccgccag ttccagctgc
     4861 cagccaatcc gttgttcagc aaaaaaagtt gaccagcgac gttgccaggt gttgggctgg
     4921 ggagttgtgg agagcgcgtt atcgaaatcg aggccaaatt gcggttggtc gctccattga
     4981 tgtaaacgcg caatttgctg accaagaata aatgcgctat gcgcatccag cgggcgaggt
     5041 gggagatagt ccatcaccag aaaactgtag tcacggtcag cgccaactgc ccaaacctta
     5101 ggcacggtga cggttttact acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa
     5161 ccgggaagca gttccctttc atcacatttg acgaaaaagt cacgtcctgc atagcgcaaa
     5221 tgccatgcgg catggacttc tccgccaggc agttcattac gcagttcgat ttcgccttca
     5281 cctaactgct cgctcaaaag acgactgatt gcctgccaca tgatgtctct cccatgttgt
     5341 ctgccagatc ataaggttag cgcaagaatg cggtgtaaaa acacgagcta acgcacacca
     5401 cagactgatt attgcccaaa aaccatttat tttctgttgc tattccattc ctcccaggtc
     5461 gtgatggtga tttcaggatc tttgccggtt gcactttggg taagccccga aaccagttct
     5521 ctaatttctt cttcactttg ggtactgata agtccaaaag tgttagtccc cagctcatgg
     5581 atatttccat catcatccgt catggtgagc aaaaaaccgt cgcgagtcag gtaattattc
     5641 aactcgttaa tttcagtcaa tgtatcctcg tgcaacatga cggttatgac ataacggaca
     5701 agatcgccac tggccatagt tcaccccttt gttatcacat gacttttgag catagtcgga
     5761 gaaacgcgtt gccgacaggt tggtggtgat tcccccaatg cagggggaat gtttcagtta
     5821 gcgggaaagg taagcgtaaa ttttttgtgt atcgtcttgg gagcacaggc gtgttccctg
     5881 attgagtgtg gctgcactcc ccgcagctac gccaaaacgg accatctctt caagagaggc
     5941 attttctgcc agtttcagtg tcatcgcgcc gaccatgctg tcaccagcgc caacggtgct
     6001 ctggcttttc accggtggtg gcacaacctg aatgcagttt tcactatcaa cgcccagcgc
     6061 cccttgtgga cccagggaaa cgacaactcg tttggccttg ccgctattaa cgatttcctg
     6121 cgcggctttg cggacatcgt ctggctgggt gagttcgcga ttcactagcg cactgagttc
     6181 tttttggtta ggcttaacca actcgatatt accaatcgcc agtgctgcac ttaacgcttc
     6241 gccagaactg tcgacgatgc agcgaatccc ttgtttttgc gcagcggaaa tcagttgggt
     6301 taatttttcc agcttcacac ctggcggcag gcttccgctt atgaccagaa tggccccgga
     6361 ttcaatttcc agaacttgct cttcaagctg gcgaaactca tcttcattta atgccgcg
//
LOCUS       DAYPHN010000215         6362 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000215 DAYPHN010000000
VERSION     DAYPHN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.215"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..170)
                     /locus_tag="ACG0BW_004413"
     CDS             complement(<1..170)
                     /locus_tag="ACG0BW_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261286.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVE"
     gene            complement(180..1070)
                     /locus_tag="ACG0BW_004414"
     CDS             complement(180..1070)
                     /locus_tag="ACG0BW_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261287.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(1067..1732)
                     /locus_tag="ACG0BW_004415"
     CDS             complement(1067..1732)
                     /locus_tag="ACG0BW_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFK7261288.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHS"
     gene            complement(1729..2397)
                     /locus_tag="ACG0BW_004416"
     CDS             complement(1729..2397)
                     /locus_tag="ACG0BW_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFK7261289.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(2397..3077)
                     /locus_tag="ACG0BW_004417"
     CDS             complement(2397..3077)
                     /locus_tag="ACG0BW_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFK7261290.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            3160..4719
                     /locus_tag="ACG0BW_004418"
     CDS             3160..4719
                     /locus_tag="ACG0BW_004418"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFK7261291.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQMVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAENGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            4722..5000
                     /locus_tag="ACG0BW_004419"
     CDS             4722..5000
                     /locus_tag="ACG0BW_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261292.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQVKLKEKNIKMSKAEVINTI
                     LEQLTMADFDKVISSVGASAKTREKIMRIYENSNMTKEDLETLLSRLK"
     gene            5027..5491
                     /locus_tag="ACG0BW_004420"
     CDS             5027..5491
                     /locus_tag="ACG0BW_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261293.1"
                     /translation="MKSPILFTFCSKTIMKYSLLPLLTLPVLMLTACQSRPISIHDAK
                     PAPQAKVFKYQSAAPATLVVMRDTGFVGAGCDASIFINGETVAKLATGEKATFHLNAG
                     ELIVGASLEGAGLCALNHARQERETTLKNGDTKAFRVFTSNSGDIDILPTTL"
     gene            5496..6020
                     /locus_tag="ACG0BW_004421"
     CDS             5496..6020
                     /locus_tag="ACG0BW_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7261294.1"
                     /translation="MTTKDITYGIQAELWPRDYTNVEKLLMFWRREQIPVRVTLEDGQ
                     AFCMYVYGLMPSRNKVDLCPAPFDKENRIRLPLERISTIESGVVDSIAHDFKGRLTVH
                     PDYVDNRPSRRDFFAICNQAYKANKSIRVYMADGREIEGVSAGADACQVTLRVENGRK
                     IVVLFDWVERILPF"
     gene            6081..6332
                     /locus_tag="ACG0BW_004422"
     CDS             6081..6332
                     /locus_tag="ACG0BW_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261295.1"
                     /translation="MDYKPVIQSLMNDVCSTSQNVSVCMYQFSAAVKAGKAIGENVEL
                     CKKVANEERAMLDCESSESSAQFVDALFDTNRKAVESVQ"
BASE COUNT         1707 a         1521 c         1468 g         1666 t
ORIGIN      
        1 ttctccacca gccccttttt tatcagagcg cgaattgaga actggatact ttgcttggtt
       61 gtcttgtacg gcaaaacttc aagcaattcg tccagatcaa gtagatgacc tcgctcataa
      121 ccgagattga gcgttttgat gatgtccttt tgtttgtcgg ttaacgtcat ggcaaatcct
      181 tatgccggta aagcaatttc taacggttta tccaaaggtt gtttgtcgaa tgccagcagt
      241 ggcagcgtgt caggcagtcg acggccaaag tcagggtttc ggtacacacc atacaacgga
      301 gacgtaaagc tcaggttgtg aatgtccttg agcagcttca caatgctggc ctcgtccacc
      361 agactgtcgg caatgtcctg aatcgtcgtg ccacgattcc gcccagcttt tgccagggaa
      421 ctgttcttgt ggtagtccgc caccagatca cgcagtgcac ggcgacgacg agactcgctc
      481 attgcgaaca actctttgac gatcgcctcg ttatcacccg ggtcggaacg gaaatggcgc
      541 tggaatacac gcagtgcact ttcatagctc ttcggtcgct cagggcggat gaacttaaac
      601 cctgctttca tggcgaaggg attgtatttg ctcatcgagg actggatctc aatgattggc
      661 cggtcatgca tcctgctaac caggttaatc attcgataag agacgcctac gccacgatac
      721 tgagtatcca caactgagcg actgatcacc gcaaagttgt tattcacgta ccgaccccag
      781 tactggttag ccacggtggt attagtggtt ggtttcaact taggaaacat gcggtggcga
      841 ggtgccaaca gtagtttcgg gtaagccata accacgacgc ccaccagacg gtcatcaagt
      901 tcgcagcgat agtaagttgg cgcgaaaggt ttgccgtctg ttttgtagtg aagcgactta
      961 agagcgtgcc agtcctctac agttcccttt gtaacagtca ttcgctccag aaagtccaga
     1021 tgacgcggaa actcttcagg gcggtagcgt ttaatgatga tgtccgtcat gaatggacct
     1081 catcatgact accattgtga tattccacct tcacgcgttc tttgtagtgc ttggtgatct
     1141 gcatatccgg gcgcagcgcg ttcttcaggt cttcgtgagt cgtcgccacc attaccgtcg
     1201 caccaacctt tcgcgcggca cgctggaggt tagacgccac aacctgagcg gttacacggt
     1261 cgagaacagc gccgaactcg tcagcagccc acactttagc gcctgactca atcagtttgg
     1321 caatcttgag acgatatttc tggccgtctg acatttcaga aggcttgcga acaaacagat
     1381 atgcatcgtt cagaccagcc atagataaca acccaagcgc atcactggtc gttttgccca
     1441 actgatcgat gacgttaacc tcattatcga aggtaaaatc atcgatggag gctacagaca
     1501 gcccttcatc cttcatctgt cgttgcaact cgcgcagcac aacggatttg ccggaaccgg
     1561 attggccggt gatgtacacc acatcgccct gcttcacttc cagctccaga ttgtcgtaaa
     1621 gcgtccactc tttttcgtcc aggccaagcc cgaacgactc agcgatttcc aacgtgcgcg
     1681 tggttttatt tacgcgtgtc tgaaacgata cgttgatgat gtatttgctc atgcagccat
     1741 ctccccggaa gagatcttct ccgcatatgc cacaaatgcg tctaccccgc tttctcccgt
     1801 catttcttcc atgtgggcaa gcaaatcacc aacaacaatg gcagagccag cagggagcgt
     1861 tttaaagccc aatacgtcga caacacgtac ttcttccgct gcaacttcac gactgatctc
     1921 ggtgtgttca tccttctgtc gtttagtttc ttcgccaaga tcgataacta gcgagtcggt
     1981 gtccatttct tctgtcatac tgccaacgag aacattcaac tcacgctctt caaagccgaa
     2041 aacctcgata tcgtccagaa cgagcgactc aagctctttc tgtagcttaa ttgcatcgta
     2101 atcaatgctg gcaagtcggt tatcttcaag gcgcttcgca cgaacctcgt catcactgag
     2161 atcatcgcga acaataaccg gtacgcgctc aagtccagca aaaattgcag cctcacggcg
     2221 gccgtggcca gtaataatta cgtcgttctt atcgaccgta attggctggt caaatccgcg
     2281 ctttttaatg gctgcggcca gatctcggat ctgctgttca tcatgctttt tggcattcat
     2341 ctcataggga ataagatctg ccgggtcgcg atatacgatt tcaaactttt tggtcattac
     2401 atacgctcct tgtagtagtc gaccagccac accagagcct caccagcgtt ctccatttca
     2461 ttaccggtgt taatagcctg ctctttgatg atgtttttta tggtttctgc aacacgatct
     2521 gacgcatcga aagttacttt gaagcgcatg gtctgatgtt ccgcacccac acgttcggtt
     2581 ttctctcgtt tgtcggtatc gacaggctca tcactaccac gagacaacgc ctccagtgct
     2641 tcaaggtcga ttgccgcctc ttttgctaaa accatcgaga tttcgtcgtc atacggggcg
     2701 atttcagaca gttgatagtc aagttctgac tgaatttctt caatgaagcg ttgcaatgcg
     2761 atttggtcgt cttcaccgta tcgctcgttg tccaccagtg acatctgttt agctacgaca
     2821 tcgctaattt tgcccacaga aagcaccgga accgttgaaa ttccttgctc aatagcagca
     2881 cgccagcgat gttcgccgcc gaggatttca aaaaatccat cttcaagttc acgagccaaa
     2941 attggcttaa aaaagcccaa tttttcgata gaacctttca gtttttcaaa attctgcgca
     3001 ccaaccgaat tggtgttcca ggtattcggg cgaaggttgg caacatcaac ctgcaaaatc
     3061 gtgattttta catccatttt attgctacaa tccactaagt aatcacttac ttattataat
     3121 agccaaataa catacaaaag gcactaagga aagaggttta tgactgttcg gattgtatct
     3181 aacgcagtta atgcgcttat ttctggcgca gatgacaagg taaagcaaat ggtgcaacag
     3241 atgttgagct acgaagtcga gactggcgac tggaagggca caagcacgat gttcaactgg
     3301 agtaaaaact cgttccctgc tggctttgcc aagcctgtag cggcgaactt gaacaaggcg
     3361 ggcatcaaat gtgttcatat ccgcaaagac aaagccccgg cgcttggtaa accaaatcct
     3421 gcggttaacc cattcccata caatcctgat tatgcgtatc aggatcagac tgtggaaaca
     3481 ctggttcgag agggaatgat gattgcgcag atcgctactg gtggcgggaa atctaacgtt
     3541 gcctgcaaag cagctgcacg tatcggtcga atgacattat ttttaacaac ccgctctgtt
     3601 ctgatgtttc aaatggccga aaacttccag agatccatcg actaccgcgc cgaaaatggc
     3661 gaaccgtggt taaaagacca aaaggttgga gtcattggct cgggtgagtt ccaggtatca
     3721 cgacatatca acgtcgctac agttcaaact cttgcaagtt tcctcgaaga accaccacgc
     3781 gatgcaacac cagataagaa aagctaccac ctcaaacgtc gggagttggt gaaacgcttc
     3841 ctttcaagtg tctctcttct tattctggaa gaggcgcatg agtcttcagg ctcaaatttc
     3901 tatgacatcg ccagattatg tgtgaacgca gactatcgtc tggcgcttac agccacgccg
     3961 ttcatgaagg attcgacgga agccaacatg cgcctgatgg cggtggccgg gcgaattgaa
     4021 attaaagtca cagaaaagta cctgattgat cgaggcattc tggcaaagcc gtactttctt
     4081 tatcataaag ttgcctacaa gccagacgag gccagaatca aggccgaact tgccaacaaa
     4141 cacctcaatt ttagagttgg tatgagcacc gcctaccaaa aggcttatca gttggggatc
     4201 gtgtataatt tgggacgtaa cgaggccatt gttcgcgaag cattgctcta taagcaacat
     4261 tctctcaatt gtatgactct ggttcgtctt aaacgccacg ggcaaatcct gatggaaatg
     4321 atgaaagagt ccggccttag agttgacttc atctatgggg aatctaacca ggcgacaagg
     4381 caagcaaagc tgaacagttt agcgtctgga gaaatagatg ttttaatagg ctcgactatt
     4441 ctggatgtcg gtgttgatgt gccaagcgtt ggtgcggtca ttcttgctgg tggtgggaaa
     4501 gcagaagttg aaatgaggca gcgtgtcggt cgtggcttac gagccaaaaa aaatcaggca
     4561 aacgtgtgtt ttatcactga tttcattgac attagcaaca aatacctgtt gtctcactct
     4621 tatgagcgaa aacacatcat cgacaccaca cctggctttg cagaaggtgt attgcctatt
     4681 gatggagcat tcgattttgg agttctgaaa cgagattagt tatgagcgaa aagaaaacaa
     4741 cttattgtca ggtagcattg tctgataagg ccaatgacaa acttggaaag tttcaagtga
     4801 aactaaaaga aaaaaatatc aaaatgtcta aggctgaagt cataaatacc attctggaac
     4861 aattgacaat ggccgacttt gacaaggtta tatcttctgt cggggcttcc gctaagactc
     4921 gtgagaaaat catgcgtatc tatgagaact ctaatatgac aaaggaagat ctcgaaacgc
     4981 tattaagcag attaaaataa tcacgctatc aattagggag acaaagatga agtctcccat
     5041 actgttcact ttctgttcta aaactatcat gaaatactcg cttctccctc tactaacgtt
     5101 accagtgttg atgcttaccg cgtgccaatc acgcccaata tcaatccatg acgccaaacc
     5161 agcaccgcag gccaaagtgt tcaagtatca aagcgcagcg ccagctacgt tggtggtaat
     5221 gagagacaca ggttttgtag gagctggatg tgatgcttcc attttcatca acggcgaaac
     5281 tgtcgcaaaa ctggctacag gcgaaaaagc gactttccat ttaaatgccg gagaattaat
     5341 tgtgggtgca tctcttgaag gtgcaggttt atgtgctcta aaccacgctc gtcaagagcg
     5401 agaaacgact ctgaagaatg gagacacaaa agcgttcagg gtgttcacca gcaattctgg
     5461 tgacatcgac attctgccaa caactctgtg atgacatgac aaccaaagat attacctacg
     5521 gtatccaagc tgaactctgg ccgcgagatt acactaacgt tgaaaaacta ctgatgttct
     5581 ggcgcagaga acaaattcct gtaagggtca ctctcgaaga tggccaggcg ttttgcatgt
     5641 acgtttatgg cctcatgcca tctcgtaaca aagttgacct ttgcccagcc ccttttgaca
     5701 aagaaaatcg tataaggctc ccacttgaac gcattagtac aattgaatca ggtgtggttg
     5761 acagtatcgc tcacgatttc aaaggtcgac taacagttca ccctgattat gtcgataatc
     5821 ggccatcacg ccgcgatttt tttgcaattt gcaaccaagc ctacaaagca aacaaatcta
     5881 taagggtgta catggcggat ggccgtgaaa ttgagggggt gtcagcaggc gcagatgctt
     5941 gtcaggttac actacgtgtc gagaacggta gaaaaatagt tgttttgttc gattgggttg
     6001 aacgaatttt gcctttttga gttatgaaat cgatattgtt accaccatta tttttacttt
     6061 gctcagttgc ggcaaatgca atggactata agcctgtcat tcagtcgctg atgaatgacg
     6121 tgtgctcaac gtctcagaat gtatcagttt gcatgtatca attttcggca gccgtaaaag
     6181 caggaaaagc gataggtgag aatgtggaac tgtgtaagaa agtggcaaat gaagaacggg
     6241 cgatgttgga ttgcgaatct agcgagtcat cggcacagtt cgttgatgcg ctatttgaca
     6301 ccaatcgtaa ggctgtagag tctgttcaat aaatctataa ggttaataac cggctaagtc
     6361 cg
//
LOCUS       DAYPHN010000216         6338 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000216 DAYPHN010000000
VERSION     DAYPHN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.216"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1045
                     /gene="rlmC"
                     /locus_tag="ACG0BW_004423"
     CDS             <1..1045
                     /gene="rlmC"
                     /locus_tag="ACG0BW_004423"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFK7261296.1"
                     /translation="TADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLL
                     GMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTES
                     QSDGGMMLRFVLRSETKLAQLRKALPWLQEQLPQLKVITVNIQPVHMAIMEGETEIYL
                     TEQQALADRFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVNHMWDLFCGVGG
                     FGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELV
                     LVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPH
                     TAHYEVLTLLVKM"
     gene            1326..2669
                     /locus_tag="ACG0BW_004424"
     CDS             1326..2669
                     /locus_tag="ACG0BW_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFK7261297.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGVLTGIFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQMMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLIHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            2691..3011
                     /locus_tag="ACG0BW_004425"
     CDS             2691..3011
                     /locus_tag="ACG0BW_004425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFK7261298.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYEFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKHGQYLIPIENIMDGETIGAQIYEVVQQHFPH
                     LLNK"
     gene            3024..4505
                     /locus_tag="ACG0BW_004426"
     CDS             3024..4505
                     /locus_tag="ACG0BW_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFK7261299.1"
                     /translation="MKAIILLFDSLNKRYLPPYGDLLTKAENFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWQASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQVETFDPHEPFHTTQEYLSLYEDDWS
                     GPHYDWPRGRVLESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSAAMFGVFSGHVNVTDGRYVYMRAALPGREDDI
                     VNYTLMPIKMNSRYPVDEMRTLSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIDDDAIEARMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(4556..5287)
                     /gene="artJ"
                     /locus_tag="ACG0BW_004427"
     CDS             complement(4556..5287)
                     /gene="artJ"
                     /locus_tag="ACG0BW_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFK7261300.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5513..6181)
                     /gene="artM"
                     /locus_tag="ACG0BW_004428"
     CDS             complement(5513..6181)
                     /gene="artM"
                     /locus_tag="ACG0BW_004428"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFK7261301.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLIVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(6181..>6338)
                     /locus_tag="ACG0BW_004429"
     CDS             complement(6181..>6338)
                     /locus_tag="ACG0BW_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226072.1"
                     /note="with ArtPMJI transports arginine across the inner
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFK7261302.1"
                     /translation="MLQTKSIATRTQEPFTWYIVAAAIYLVITLLSQYILKRIDLRAT
                     RFERRPG"
BASE COUNT         1572 a         1490 c         1627 g         1649 t
ORIGIN      
        1 aaccgccgat cttaaaaatc tgctcgccga ctttccggtt gaggaatggt gcgcgccggt
       61 gtcaggcccg gaacaagggt ttcgtaataa agccaaaatg gtggtgagtg gtagcgttga
      121 aaaaccactg ctcggtatgc tgcatcgaga cggcacaccg gaagaccttt gtgactgccc
      181 gctttatcct gcctcatttg cgcccgtttt tgcggcactg aaaccattta ttgctcgcgc
      241 cgggcttacg ccttacaacg tggcgcgcaa acgtggcgaa ctgaaataca ttctgctgac
      301 cgaaagccag agcgatggcg gcatgatgct gcgctttgta ctgcgttctg aaaccaaact
      361 ggcgcaactg cgtaaggcgc tgccgtggtt acaggaacaa ctgccgcagc taaaagttat
      421 taccgtcaat attcagccgg tacatatggc gattatggaa ggggagacgg agatctacct
      481 gaccgaacaa caggcgctgg cggatcgttt taacgatgtg ccgctgtgga tccgtccgca
      541 aagtttcttc cagactaatc cggcggtcgc cagccagctt tacgctaccg cgcgcgactg
      601 ggtgcggcaa ctaccggtaa accatatgtg ggatctgttc tgcggcgtgg ggggctttgg
      661 tttacactgc gcgacgcctg acatgcagtt aaccgggatc gaaattgcac cagaggccat
      721 tgcctgtgcg aagcagtcag ccgctgaact gggcttaacg cgtttgcaat ttcaggcgct
      781 ggactccact cagtttgcta ccgcccaggg ggaagtgccg gagctggtgc tggttaaccc
      841 gccgcgccgc ggcattggta aaccgctatg tgattatctc tcaacgatgg caccgcgttt
      901 tatcatctac tccagctgta acgcccaaac catggcgaaa gatatccgcg agctgccggg
      961 ttatcgcatc gaacgggtac agcttttcga catgttcccc cacaccgcgc attacgaagt
     1021 gctgacactg ctggtaaaaa tgtaaataca tcactcaaat caatatcact ccggcataca
     1081 tccattctaa ataataaccg tgatcgatcg attaatatta accgatcgat catggttata
     1141 tattgcgcta tttttgcaaa tcaaatcaca aataatcaaa taagatcttt aaatgaactg
     1201 ttttgattac taaagtgcgt ttaccgaata cctgcagagg catcgcttag cgaaaaataa
     1261 aaaatatctg aagcattatt aacgtcagca gaaggttcat gcattgacaa cggaaatgta
     1321 cgcttatgga gggcgttccg acgatgtttg ccaaattcat agatgttatt caaacgtttt
     1381 taaccgaacc cgcaatatta attggtttgc tggtgggcat tggttatgcc cttgataaga
     1441 aatcgccaat aaaaattatt accggtatgg tcagcgccat ggtgggatta atgatggtgt
     1501 tatttggtgg gtttcaattc tccgcaacat ttaaaccggt agcagaggct gtaagtaaag
     1561 cctatggcgt tcatggttat ttaatggatt cctacgccat gaaagccgca acgcaaattg
     1621 cgttggggga taattttggt tacgtaggtt atgtttttgt tctggcattt tttaccaacc
     1681 tgttactggt actgtttggt cgctataccg gtgccaaagg gatattcctg accggtaata
     1741 ctggggtgtc acattcgcag gccgtattat ggttaattgt tttttggtta ggatttagtt
     1801 ggaccacgtc gattattatc gccggggtat taacgggtat attctgggct ttttccacca
     1861 cgctcattgt taaacctatc gccaaagtga ctaaagatgc tggttttact attgcccata
     1921 accagatgtt gggattgtgg tttttctcca aattcgccca taaatttggc gacccggaaa
     1981 agcacgatgc tgaaaacctg aaacttcccg gctggctggc gatttttaac cacaacgtga
     2041 cggctatcgc cattgtgatg acgctgtttg tgggcggttt cctgctctcc acgggcattg
     2101 ataacgtcca gatgatggcg aaaggtaaac cctggtacat ctatatcatc aatctgggct
     2161 tacagttctc catgtacatg gtcattctgt tgcagggcgt gcgcatgatg gtcggtgaaa
     2221 ttaacggttc ttttaaaggc tggcaggatc gctttattcc taacgccatt cccgccgtcg
     2281 atgtcgccgc actgttgcct ttttcaccca atgccgcaac gttaggtttt gtcttctgta
     2341 cttttggcac cattttctcg atgggtatct tattgctgat ccacagcccc attatggtat
     2401 tgccaggctt tgtaccttta tttttctcag gcggcccgat tggtgtatta gcaaaccgaa
     2461 tgggcggata ccgctccgta attatctgta ccttcttgct gggtattatt cagaccttcg
     2521 gtacggtgtg ggctattcct ttaaccgggc ttgctgaaaa tggcgtcggc tggacaggaa
     2581 tatttgactg ggcaaccgta tggcctgcta tttgtgaagt tctgaaattt atcgctgcaa
     2641 cattccatct tggtccttac gcgggataat tcactattaa gggtttaaaa atgaaagata
     2701 caattaatat tttgtttgtc tgtggttatg gtgtcggtag tagcgtgatg ttacaaacgg
     2761 ttgttaaaaa agcgctgacg aaatatgagt tcaaagttga aatggaacat accgccgcag
     2821 gcgaggtggg aggctttacc gattgggcgg atatttttgc tatttccaaa aaattactcg
     2881 acgtggtcag cctggaccca aaacacgggc aatatctgat tccgattgaa aatattatgg
     2941 atggtgaaac gattggcgcg cagatttatg aagtcgtcca gcagcatttc cctcatctgc
     3001 taaataaata aaagggatga gcaatgaaag ccattattct cctgttcgat agcctgaaca
     3061 aaagatattt acccccgtat ggcgatttat tgacaaaagc agagaacttc caacgcctgg
     3121 cggcacatgc cgccaccttt gaaaacagct acgtcgggag tatgccctgc atgcccgccc
     3181 ggcgagagct gcataccggg cgatgcaact tcctgcaccg ggagtggggg ccgttagaac
     3241 cgtttgacga ctccatgccg gagctgctta aaaaggcggg gatctatacc cacctgataa
     3301 gcgatcattt gcattactgg gaagatggcg gcgggaatta tcataatcgc tacagttcat
     3361 gggagattgt gcgcggccag gaaggcgatc actggcaggc gagcgttgcg caaccgccta
     3421 ttcccgaggt gctaagggtg ccacaaaagc agaccggcgg cggtgtttct ggcctgtggc
     3481 gtcatgactg ggccaaccgg gaatatattc agcaggaagc ggattttcca caaaccaaag
     3541 tctttgatgc gggttgtgct tttatccaca aaaatcacgc ggaagataac tggttattgc
     3601 aggtcgaaac gtttgatccg cacgagccgt ttcacacaac gcaagagtac ctttcgttgt
     3661 atgaggatga ctggagcggg ccgcattatg actggccgcg tggtcgggtg ctggaaagcg
     3721 acgaggccgt ggagcatatc cgctgccgct atcgctcgct ggtgtcgatg tgcgatcgca
     3781 acctggggcg cattctggac ctgatggatg agcacgatct gtggcgagac accatgctga
     3841 ttgtcggcac cgatcacggt tttttactcg gtgagcatgg ctggtgggcg aaaaaccaga
     3901 tgccttatta caacgaggta gcgaataacc cgctgtttat ctgggatccg cgcagcggcg
     3961 taaaaggcga acgtcgtcag gcgttggtgc agatgatcga ctgggcacct acgctgtatg
     4021 acttttttca gcagccggtg ccgcccgatg tgcaggggca accacttgcg aaaacggtca
     4081 gccacgatga accggtacgc agcgcggcga tgtttggtgt ctttagtggt catgtcaacg
     4141 taaccgatgg gcgttatgtg tatatgcgtg ccgcgctgcc gggtcgtgag gatgatattg
     4201 ttaactacac attaatgccg attaaaatga acagccgcta tccggtggat gagatgcgga
     4261 ctttatcgct ggcccctccg tttcgtttta ccaaagggtt acaggtgtta cgcatcccgg
     4321 cgcaggagaa atataagggg ctgaatcagt ttgggcattt gctgttcgat ctgcaaaacg
     4381 atccgcagca gttgcatcct attgatgatg atgcgatcga ggcccggatg atcgcgttgc
     4441 tgatccagtt aatgaaagat aatgatgcgc cgccagagca gtttcagcgc ctggggttag
     4501 cgtagagagg tgatgttgcc ggagtgggcg aacacccact ccggcaaatg aatacttact
     4561 gcgggaacca ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg
     4621 cattattcag tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg
     4681 tgccgaagta ctgcggatcg gtcactttct cagtcgcaac gcccagttgt ggattggttt
     4741 tcagccattc gtttaccacc gctgtgtcac caaatacccc atcaatacga ccatttttca
     4801 ggtcgataaa ggcattctga taactgtcat aggagacagt tttcacttcc gggtgctgat
     4861 cctgaatata tttctggtgc gtggtgccgt tttccatccc aatacgtttg cctttcagat
     4921 cggcaaacgt tttgtaggta tcttttttgg caatcacgac ggctgagttt tcatagtagg
     4981 gcgtggtaaa cgatacctgt ttgctacgct ccggcgtgat atccataccg gagattacgg
     5041 cgtcatattt tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag
     5101 tacattctgc ctgcatttgt ttgcacaagg cttttgccag gtcgatatca aagccgacaa
     5161 tctcattatt agcacctata gattcaaagg ggggataggt ggctgaaacg ccaaaattga
     5221 ttttctctgc ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact
     5281 ttttcatgat ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg
     5341 gataacaatg ccattaagtg aatttatatg caataaatat gattaaataa tttaaatgaa
     5401 ataaaaaaag acggacaact tagtgagttg tccgtcttca ttataagaat ttatgcacta
     5461 tgcaggtcgg ataagacgtc cacgacgcat ccggcatcag cacgcagcta cattaattcc
     5521 gccgttcgaa tgccagtgct ttgcgctcga tcagacgcat catcagcgtc aacagaccgt
     5581 taacaatcag ataaataatc cctgccgcac cgaacaccat gacatcgtag gtgcgtccgt
     5641 acaacaactg gctgtatccc atcacttcca tcagcgtaat ggtgtaagcc agagaggtac
     5701 ttttgaacac cagcaccact tcgttggaat aagaagagag cgagcgttta aaggcatacg
     5761 gcagcaggat cgctagcgta tcttttttgc tcattcccag ggcgctacag gactgccact
     5821 gaccttccgg gatcgcacga atggcaccgt aaaacagctg cgtggtatac gctgcactgt
     5881 tcagcgacaa cgcaatcagc gcacacagcc acggttctga cagcaaatgc cacagtgccg
     5941 gatactcctg caacgtcggg aactggcccg gcccgtaata aatcaggaag atctgcacca
     6001 gcagcggcgt accggtaaac agcgtgatat aaccccgcac cagccacacc agtaccggcg
     6061 ttttcagcgt caggatgatg gtaaaaatca acgccagaat cagtgccaca atcagcgagg
     6121 caacggttag cgtcaggctg gtgtgcagcc ctttcatcag ttcgggtaaa tactcaaaca
     6181 ttagccaggc ctccgctcaa aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg
     6241 actgagcagg gtgatcacca ggtaaatcgc cgccgccaca atgtaccagg taaacggttc
     6301 ctgggtacga gtagcaatgc ttttggtttg tagcatta
//
LOCUS       DAYPHN010000217         6296 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000217 DAYPHN010000000
VERSION     DAYPHN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.217"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..316)
                     /gene="smrB"
                     /locus_tag="ACG0BW_004430"
     CDS             complement(<1..316)
                     /gene="smrB"
                     /locus_tag="ACG0BW_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFK7261303.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQ"
     gene            482..1414
                     /gene="prmB"
                     /locus_tag="ACG0BW_004431"
     CDS             482..1414
                     /gene="prmB"
                     /locus_tag="ACG0BW_004431"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFK7261304.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            1449..2534
                     /gene="aroC"
                     /locus_tag="ACG0BW_004432"
     CDS             1449..2534
                     /gene="aroC"
                     /locus_tag="ACG0BW_004432"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFK7261305.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            2538..3362
                     /gene="mepA"
                     /locus_tag="ACG0BW_004433"
     CDS             2538..3362
                     /gene="mepA"
                     /locus_tag="ACG0BW_004433"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFK7261306.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLSLGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            3362..4171
                     /locus_tag="ACG0BW_004434"
     CDS             3362..4171
                     /locus_tag="ACG0BW_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFK7261307.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            4171..4719
                     /gene="epmC"
                     /locus_tag="ACG0BW_004435"
     CDS             4171..4719
                     /gene="epmC"
                     /locus_tag="ACG0BW_004435"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFK7261308.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLENGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            4753..5031
                     /locus_tag="ACG0BW_004436"
     CDS             4753..5031
                     /locus_tag="ACG0BW_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFK7261309.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(5152..>6296)
                     /gene="mnmC"
                     /locus_tag="ACG0BW_004437"
     CDS             complement(5152..>6296)
                     /gene="mnmC"
                     /locus_tag="ACG0BW_004437"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFK7261310.1"
                     /translation="QVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTF
                     ARQFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITG
                     VTTNCSGITYSEGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAG
                     DQQATHNVVVLANGHQISGFSQTSTLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLT
                     PQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARC
                     GVRCATRDHLPMVGNVPDYDATLVEYASLAEQKDEAVSAPVYDDLFMVAALGSRGLCS
                     APLCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         1451 a         1690 c         1653 g         1502 t
ORIGIN      
        1 gctgcgtcaa accgtgcaaa tccaaaaaca gctccggtga ataatcgcca cggcgtaact
       61 tcttcgcctc aaaatggctg acatccgggc gaacatattt caccggacct tcggtattca
      121 ataacggctg aaattcatcg gaaaaataat ggctggcatc ggcctgctcc tgaatcaacc
      181 gtttcaccgg cacttcgctg attttcttac gctgcggtcg gtggacaatc gtgtcctgct
      241 taatcttgcg agtccccgcc atcaactgtc gaaacagcgc ctggtcctcc tcgctaagtg
      301 ttgttttctt tttcatttat ctgtctctat ttctaatttt cctacagttt acccgactcg
      361 tagcgcaatc gatcgtttta aacgtatttt tcctcactca ccgcgcgtga atgctgattt
      421 atcgtcatct tcatggcaaa ctagccgccg aaattattgc gatcatgccc tggaggaata
      481 cgtggataaa attttcgttg atgaagcagt aaatgagctg caaaccattc aggacatgtt
      541 gcgctggtcg gtgagccgct tcagcgcggc gaatatctgg tacggccacg gcactgacaa
      601 cccgtgggat gaagccgtgc aactggtgtt gccttcactc tacctgccgc tggatatccc
      661 ggaagatatg cgcaccgcgc gtctgacctc cagcgaaaaa caccgtattg ttgagcgcgt
      721 gatccgccgc gtcaatgaac gcattccggt ggcttacctg actaacaaag cgtggttctg
      781 cggtcatgaa ttttacgtcg atgaacgcgt gctggtgccg cgctcgccga ttggtgaact
      841 gatcaacaat aaatttgccg gacttatcag caagcaaccg cagcatattt tagatatgtg
      901 taccggtagc ggctgcatcg ccattgcctg tgcttatgcc ttcccggatg cggaagttga
      961 cgcggtggat atctctcccg acgcgctggc ggttgctgaa cagaacatcg aagaacacgg
     1021 tctgatccac aacgtcatcc cgattcgctc cgatctgttc cgtgatttgc cgaaagtaca
     1081 gtacgacctg atcgtcacta acccgccgta cgtcgatgcg gaagatatgt ccgacctgcc
     1141 aaatgaatac cgccacgagc cggaactggg cctggcatct ggcaccgacg gcctgaaact
     1201 gacgcgtcgc attctcggta acgcggcaga ttacctcgct gatgatggcg tgctgatttg
     1261 tgaagtcggc aacagcatgg tacatcttat ggaacaatat ccggatgttc cgttcacctg
     1321 gctggagttt gataacggcg gcgatggtgt gtttatgctc accaaagagc agctgctggc
     1381 ggcccgtgaa catttcgcga tttataaaga ttaagtaaac acgcaaacac aacaataacg
     1441 gagccgtgat ggctggaaac acaattggac aactctttcg cgtaaccacc ttcggcgaat
     1501 cgcacgggct ggcgctcggc tgcatcgtcg atggtgttcc gccaggcatt ccgctgacgg
     1561 aagcggacct gcaacatgac cttgatcgtc gtcgccctgg gacatcgcgc tataccaccc
     1621 agcgccgcga gccggatcag gtcaaaatac tctccggtgt ttttgaaggt gttactactg
     1681 gcaccagcat tggcttgttg atcgaaaata ccgaccagcg ttctcaggat tacagcgcca
     1741 ttaaagacgt tttccgtcca ggccatgccg attacaccta cgaacaaaaa tacggtctgc
     1801 gcgattatcg cggcggcggg cgctcttccg cccgcgaaac cgccatgcgc gtggcggcag
     1861 gggcgattgc aaaaaaatat ctcgccgaga aatttggcat tgagattcgc ggctgcctga
     1921 cccagatggg tgacattccg ctggaaatca aagactggtc gcaggtcgag caaaatccgt
     1981 ttttctgccc ggacccggac aaaatcgacg cgttagatga actgatgcgc gcgctgaaaa
     2041 aagagggcga ctccatcggc gcgaaagtca ccgttgttgc cagtggcgtc cccgccggac
     2101 ttggcgagcc ggtctttgac cgcctggatg ccgacatcgc ccatgcgctg atgagcatca
     2161 acgcggtgaa aggcgtagaa attggtgatg gttttgacgt ggtggcgctg cgtggcagcc
     2221 agaaccgcga cgaaatcacc aaagacggtt tccagagcaa ccatgcgggc ggcattcttg
     2281 gcggtatcag cagcgggcag caaatcattg cccatatggc gctgaagcca acctccagca
     2341 ttaccgtgcc agggcgtacc attaaccgct ttggcgaaga agttgagatg atcaccaaag
     2401 gccgtcacga tccttgtgtc gggatccgtg cggtgccgat cgcagaagcg atgctggcga
     2461 tcgttttaat ggatcacctg ttacggcagc gggcgcaaaa tgccgatgtg aagactgata
     2521 ttccacgctg gtaaaaaatg aacaaaaccg cgattgcgct gctggccctg cttgccagta
     2581 gcgccagcct ggcagcgacg ccgtggcaaa aaataaccca acctgtgccg ggtagcgcac
     2641 aatcgatagg cagtttttct aatggctgta tcgtcggcgc tgacacgctg ccgatacagt
     2701 ccgaacatta tcaggtcatg cgtaccgatc agcgtcgcta tttcggacac ccggatctgg
     2761 tgatgtttat ccagcgtctg agtagccagg tgagcaatct gagcttgggc acggtgctga
     2821 ttggcgatat ggggatgcct gctggtggtc gtttcaacgg cggtcacgcc agccaccaga
     2881 ccggactgga tgtggacatc ttcctgcaac tgccaaaaac acgctggacc tccgcgcagc
     2941 tcttgcgccc gcaagcgtta gatttagttt cccgcgacgg taaacacgtt gttccgacgc
     3001 tgtggaagcc agaaattttc agcttgatca aactcgccgc ccaggacaaa gacgtcacgc
     3061 gcatttttgt taatccggcg attaaacaac aactttgcct tgatgcgggc accgatcgcg
     3121 actggttgcg caaagtgcga ccctggttcc agcatcgcgc gcatatgcat gtacgattac
     3181 gttgtcctgc cgatagtctg gagtgtgaag atcaaccttt accgccacca ggcgatggtt
     3241 gcggggcaga actgcaaagc tggtttgaac ctccaaaacc gggaacaaca aagcctgaga
     3301 agaagacacc gcctccgttg ccgccttcct gccaggcgct actggatgag cacgtgatct
     3361 aatggaaacg tttaatagcc tgtttatggt ttccccgctg ttgctgggag ttctcttttt
     3421 tgtcgccatg ctggcgggat ttatcgactc gattgccggt ggcggtgggt tactcaccat
     3481 tccggcattg atggcggcgg ggatgtctcc cgctaacgcg ctggcaacca ataaactgca
     3541 agcctgcggt ggctctattt ccgccactat ctactttatt cgccgcaaag tggttagttt
     3601 aagcgatcag aaactcaata tcgccatgac ctttgtcggc tcaatgagcg gcgcattgct
     3661 ggtgcagtat gttcaggctg atgtcttacg gcagattttg cccattctgg tgatttgtat
     3721 cggcctctat ttcttgctga tgccaaagct gggtgaagaa gatcgccagc gccggatgta
     3781 cggtttaccc ttcgcgttaa ttgccggtgg ctgcgtcggt ttttatgacg gcttctttgg
     3841 tccggcagcg ggatcgtttt acgcgctggc ctttgttacg ctgtgcggat tcaacctcgc
     3901 caaagccacg gctcacgcca aattactcaa cgcaacgtca aatatcggcg gtttactgct
     3961 gtttattctc ggtggcaaag tgatttgggc gacaggattc gtgatgctgg tggggcagtt
     4021 cctcggcgcg cgcatgggtt cacgactggt gttgagcaaa ggacaaaagc tgatccgccc
     4081 gatgattgtt attgtctcag cggtgatgag tgccaaatta ctttacgata gccacggaca
     4141 ggagatcctc cactggttgg ggatgaacta atgaacagta cacaccacta tgagcagttg
     4201 attgaaattt ttaatagctg ctttgccgat gattttaata cccgtctgat taaaggcgac
     4261 gacgaaccga tctatcttcc tgctgatgcg gaagtaccgt ataaccgaat cgtctttgct
     4321 catggctttt atgccagcgc catccacgag atttcgcact ggtgtattgc cgggaaagcg
     4381 cgccgtgagc tggttgattt cggctactgg tattgcccgg acggacgcga tgcccaaacg
     4441 caaagccagt ttgaagatgt tgaagtgaag ccgcaggcgc tggactggtt gttctgtgtg
     4501 gcggcgggat atccgtttaa cgtcagttgc gacaatctgg aaggcgactt cgaaccggat
     4561 cgtgtggtgt ttcaacggcg ggtacatgca caggtgatgg attatctgga aaacggcatt
     4621 cctgaacgcc cggcacgctt cattaaagca ttacaaaatt attatcacac gccggaactt
     4681 acggcggaac agttcccgtg gccggaagcg ctcaactaaa gccagcgcca cgttttacaa
     4741 tgaggaaaga agatgatcgc ggagtttgaa tcacgcattc tggcattaat cgacggtatg
     4801 gttgaccatg ccagtgatga tgagttgttt gccagtgggt atttgcgtgg ccacctgaca
     4861 ttagccatcg cagaactgga aagtggtgat gaccactccg cccaggcggt acatacgacc
     4921 gttagccaga gtctggaaaa agccattggt gccggtgaat tgtcgccgcg tgaccaggcg
     4981 ctggtgaccg atatgtggga aaacctgttt cagcaggcgt cacagcagta agttgcggcg
     5041 tctggagcac catgcctgat gcgacgctta tcgcgtctta tcaggcctac acgtcacacg
     5101 taggtcggat aaggcgttca cgccgcatcc gacaaacact gccaaagctg gttacccagc
     5161 cttaactgct ttacccttca acaatttccg cacccataac ctattcggat ttaacgccgc
     5221 cagcgtactg gcatccatcg gaatcggttc ttcactcatc tgcgccgcca gaatctcggc
     5281 acacagcggg gcagagcaca aaccgcgaga acccaaagca gcaaccataa agagatcgtc
     5341 ataaaccggt gcgcttaccg cctcatcttt ctgctccgcc agcgacgcat attccacgag
     5401 tgtagcgtca taatcgggaa cattacctac cattggcaga tgatcgcgag tggcacaacg
     5461 cacaccgcag cgcgcctctt tatcactgac atcaacctct tttgcccact gcgcctgcgg
     5521 gaaacaatca atcaaccgct ggcgattctg ctgctgatcg tcctcactgt acgccgtatc
     5581 ttcgctgccg cgatgataac tggcaccaat acaatgatgt tgattcgccg gattttgtgg
     5641 cgtgagataa ccgtcatagc acagcacctg cttcagtttt gccagttccg gcgttgtcgg
     5701 aatatggctg acctgtccgg caaccgaata cactggcaga gtcgatgttt ggctgaaacc
     5761 gctgatttga tgcccgttcg ccagtaccac tacattgtgt gttgcttgct gatctcctgc
     5821 aaaattcagc aaccaacagt catccttacg ggataaatcc tgcaactgat attgataatg
     5881 aatctgtaaa ccttgctgtt gcgccagttc cagcacatta cgggttagtt ctgccggaca
     5941 cagccagcca ccttccgaat aagtaatacc gctgcaattt gtcgtaacgc ccgtaatttg
     6001 ttcaaccgca tttgcctcaa cggctacagc cagttcttcg ggtaaatcca ttgacagcat
     6061 ctgtgcgatt ttatgctggc ttttctcatc ccagcctaac tgcgtgacgc cgcaccagtc
     6121 atgatcaaat ttaacgggta attggtcgta aaactgacga gcaaaagtaa acgcattaga
     6181 gaaaaagcgg ttcagcgcct catcgtgttt acttaagagc ggatacagcg ccccctggcg
     6241 attgccggaa gcacccagtg cgggggcctc atccgcgcaa taaagtgtta cctgcc
//
LOCUS       DAYPHN010000218         6225 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000218 DAYPHN010000000
VERSION     DAYPHN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.218"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1341
                     /gene="paoC"
                     /locus_tag="ACG0BW_004438"
     CDS             <1..1341
                     /gene="paoC"
                     /locus_tag="ACG0BW_004438"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFK7261311.1"
                     /translation="QHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGA
                     NRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVD
                     PADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEK
                     SGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSA
                     GSGGQWGANTSTSGVYAACVKLREMIASAVGFDPGQSQFADGKITNGTRSATLHEATA
                     GGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAVRILNPK
                     TARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDT
                     DPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1351..2307
                     /gene="paoD"
                     /locus_tag="ACG0BW_004439"
     CDS             1351..2307
                     /gene="paoD"
                     /locus_tag="ACG0BW_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFK7261312.1"
                     /translation="MSYSLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEVMGSGRDREICYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVSLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQDEDSCLPPSS"
     gene            complement(2488..3615)
                     /locus_tag="ACG0BW_004440"
     CDS             complement(2488..3615)
                     /locus_tag="ACG0BW_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261313.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGAQA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALASSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSSILLIIGVAGFIGTLVVSAFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLAIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3757..4815)
                     /locus_tag="ACG0BW_004441"
     CDS             complement(3757..4815)
                     /locus_tag="ACG0BW_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFK7261314.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWQDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5080..5964
                     /locus_tag="ACG0BW_004442"
     CDS             5080..5964
                     /locus_tag="ACG0BW_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7261315.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
BASE COUNT         1451 a         1724 c         1733 g         1317 t
ORIGIN      
        1 cagcacctgc gtatcggtgc cgaccagagc gggaaaatca ctgctatctc acatgaaagc
       61 tggtccggaa acctgcccgg cggcacgccg gaaacggcgg tacagcaaag cgaattactc
      121 tacgcagggg cgaatcgtca taccggcctg cggctcgcca cgcttgattt gccggaaggg
      181 aacgccatgc gtgcgcccgg cgaagccccc ggtctgatgg cgctcgaaat cgcgatcgat
      241 gaactggcgg aaaaagcagg catcgatccc gtcgagtttc gcatcctgaa tgacactcag
      301 gttgaccccg ccgacccgac gcgccgcttc tctcgccgtc agcttattga gtgcttgcgc
      361 accggagcgg ataaatttgg ctggaagcag cgcaacgcca cacccggaca ggtacgcgac
      421 ggggagtggc tagtcggcca cggtgttgcg gcgggctttc gcaataatct gctggaaaaa
      481 tcgggtgctc gggttcacct agaacaaaac ggcaccgtta ccgtggaaac ggacatgacc
      541 gatattggca ccggcagcta cacaattctg gctcagacgg cagcggaaat gcttggcgta
      601 ccgctggagc aggttgcggt tcacctcggc gattccagtt tcccggtttc tgcgggttct
      661 ggtggacaat ggggcgcgaa tacctccacc tccggcgttt acgccgcctg tgtgaaactt
      721 cgcgaaatga ttgcctcggc agtcgggttt gatcctgggc agtcgcagtt tgccgacggc
      781 aagattacca acggtacccg aagcgccacg ctacatgagg ccaccgcagg cggcaggctg
      841 acagcggaag agagcattga attcggaaca ctgagcaagg agtaccagca atcgaccttt
      901 gccgggcatt ttgtggaggt cggcgtgcat agcgcgacgg gagaagttcg ggtccggcgt
      961 atgctcgctg tgtgtgctgc agtacgcatc ctgaatccga aaaccgcacg cagccaggtc
     1021 attggcgcaa tgactatggg catgggcgcg gcactgatgg aggagctggc ggtggatgac
     1081 cgtttgggct acttcgttaa tcacgatatg gcggggtatg aggtgccggt tcatgcggat
     1141 atcccaaaac aggaggtgat tttcctggat gataccgacc ccatctcctc cccgatgaag
     1201 gccaaaggtg tcggtgagct gggcctgtgc ggcgtgagcg cggctatcgc caacgcggtg
     1261 tataacgcca ccggtattcg ggtacgggat tatcccatca ctctggataa gctgctcgat
     1321 aaactgccgg atgtggttta aggaggaaca atgtcatact cgctttttga caaagacgag
     1381 cactggcata agccagagca ggcgtttctc accgatgacc accggaccat tctgcgcttc
     1441 gccgtagagg cgctaatgtc cggtaaagga gcggtgctgg tgacgctggt ggagatacgc
     1501 ggcggcgcgg cgcgcccgct cggggcgcag atggtggtgc gcgaagatgg ccgttactgc
     1561 ggttttgtct ctggcggctg cgtggaggcc gctgccgctt ttgaagcgct ggaggtgatg
     1621 ggctcaggcc gcgatcgcga aatttgctat ggcgaaggtt cgccgtggtt tgacatcgtt
     1681 ctgccctgcg gcggtgggat cacgctgacg ctccataaac tacgctcggc acagcctctg
     1741 ctcgccgtgc tgaaccgcct ggaacagaga aaaccggcgg gtctgcgcta cgatccgcaa
     1801 gctcaatcgc tggtgtgcct gcccacgcaa acccggacgg gctggaatct caatggcttt
     1861 gaggtggggt tcaggccatg cgtcagtctg atgatttacg gacgttctct tgaggcgcag
     1921 gcaaccgcga gtcttgcagc agccacaggc tatgacagcc atatcttcga tctttttccg
     1981 gcctcagcca gcgctcagat cgataccgat acggcggtca ttttgctgtg ccatgatctc
     2041 aaccgggagc tgccagtgtt gcaggccgcg cgagaagcaa aaccctttta tctcggcgca
     2101 ttgggcagct atcgaaccca cactttacgt ctgcaaaagc tccacgagct gggatggtcc
     2161 agggaggaga cagcgcaaat ccgggcaccc gtcgggatat tccccaaagc ccgggatgcg
     2221 catactctgg cactctccgt gctggcagaa gtcgcctctg tacgtctcca tcaggatgag
     2281 gattcatgcc tgcccccgtc gtcctgatcc ttgcggccgg gcgtggagag cgctttctcg
     2341 cctccggggg aaatacccat aaattaagag aaaaaatctg cagtagccgt tttagaaaat
     2401 cacctaaatc gtttaaaaga gcgcccaacc catagtgggc gctcatcgcc ttgtgtaact
     2461 tattcagcaa cgcgcgccct cgccggacta aagcgcaccg gccttctggc gtgcggtaac
     2521 aaaggtcagt acgccagcgc aaaggagcag cacgctactc atagcaaacg tgctctgcca
     2581 gccaaggcgg tcaaacacaa tgcctcctgc cgttgatcca agggcgatgg cgagctgaat
     2641 caccgcgacc atgagcccgc ctccagcttc ggcgttgtca ggcaacgtac gcgcaatcca
     2701 tgtccaccat ccggttggcg cggcggtggc cagcatgccc cagagtccta acagcagcga
     2761 gaccgtccag acgtgatgcc cggtaagcat cagaccgatg gcaatgacgg ccatcagtcc
     2821 gggtatggcc atcagcgtgg ggtagaattt tctgttgaga aatgccgaaa caaccagcgt
     2881 gccaataaaa ccggccacgc cgataatcag caaaatcgac gacaagccta aggaattaac
     2941 ccgtgtcacc gactccagaa acgggcgcac ataggtgaat agcgcaaact gccccatgaa
     3001 aaacaggcca caagctatca tgccgaccac aaccatgcgg cggctaaata agcggaatac
     3061 cgcgccacat gaagtgtggc ttttattcgc atccatgctt ggcaggctga tgcactgcca
     3121 gataaaggcg acaaccgcga tgggcaccag gcacaagaaa gcgcctcgcc atccgaccgt
     3181 agcccccaga tagcttccca gcggtgcggc cacgaccgtc gccagcgcat taccggcgtt
     3241 gaaaattgcc agcgcacgcg tgacctgatg ctgcggcacc agacgaatcg ccgttgccgc
     3301 tgacatcgac cagaatccgc caatagccac accaatcatc gcacgaccga ccatatacat
     3361 cagataactg gaagccagcg caataataag ccccgatacg gccatcagaa ccgtcatccc
     3421 cagcagcagg aacttacgat tcatctttcc agctagcgtt gaaagcgtca ggctggtcag
     3481 aaccgcctgc gcgccggaga tagcaatccc ctgccctgca agcccctccg tcacgcctaa
     3541 atcgcgggta atcggcgtga gcaggctgac gggcataaat tcagaagcaa tcagcacaaa
     3601 cacgcacagg gtcatggcaa aaataccgcc ccagtatgcg cgctgatgat gaggtgatag
     3661 tgtttggctt agtgtcgaca tagtgaaatt tcacaaagat caggataaaa gccaccagcc
     3721 tatggccggt ggctttgcat caattcagga gaggttttat ttcagtttgg tctggaaaaa
     3781 ttgttcgaac ttcgcgaaag gtatttttcc ggccacgtta tcgtacaggt ccacgtgatt
     3841 tgcccctggt accaccacca actctttgtc tttactgcca accgccttgt aagcatcttc
     3901 agcaaaatag cgtgaatgtg ctttctcacc ggtcacaatc agcgtcggaa tggtgatttc
     3961 actggcgtag ctcaacagcg gcatattcat aaatgacagc ggcatcgttg ccgtccacgc
     4021 cccggtagag ttaacggaac gttcgtggaa gccgcgcggc atgcgatagt agtcaaagaa
     4081 ctctttcagt accggatgag gatttgccgg cagcgtttct ggcagaatgc gctcgcccgc
     4141 actgaccttg ccgttgctat cgacattaat atcatgacca ccgtgagcga acgttccgct
     4201 ctgcgcatcc tgccagcgct gttcattcag atattgcaga acggcacgac ggtcggcggt
     4261 agaataacgg tctttgccat cccccacacc atgacccatc gcccggctca tgtcatacat
     4321 cacgctggta gctaccgctt taacgcgggt atccatcgcc acgtcgttca gcgccatgcc
     4381 gccccagcca caaataccga gcagcccgat acggttgcga tctacctctt tttgcagccc
     4441 taagaaatcc accgccgcgc tgaaatcttc agtgttgata tccggtgagg cgacgtttcg
     4501 cggatagccg ccgctttccc cggtgtaaga aggatcgaac gccagggtaa caaaccctcg
     4561 ttccgccagc gtctgcgcat acaagccgct ggattgctct ttcaccgcgc caaatggccc
     4621 actgaccgca atcgccgcca gcttgcgatc gccacggtct ttaggcaggt aaagatcgcc
     4681 cactaaggtg atgccatatc ggttcgggaa cgagacttta cggtgatcga ctttctggct
     4741 ctcggcgaac gttttatccc atttatctgt catggacaca ggggcattag gattggttga
     4801 atcagcataa ctcattgtcg tgactccact taatgatgcg aaaagcagca tggtcggcat
     4861 cgcggttttt agcgttcggg tgaaggcttt cataaaaatc cccataaaaa acaatagaga
     4921 gatacaggtt tccgacggaa aacgctgcgg tgctttgctt tgatggggtc attataatga
     4981 gcagaattag tgctgattag ggggcgatta atgctaagat taataccata ttgttataaa
     5041 ttaatgcgga gattgccggc atgaaggggg gatgtttcaa tggcgaaaag ggagaactac
     5101 aacgagctgt acctgtttat gcaggtggtg cgggaaggca gctttaccgc agcggctcag
     5161 cggctgggtc ttgctcagtc aggagtcagc cgttctgttc gcgagcttga agaaaggctc
     5221 ggcgtccagc tgctagtgcg caccacgcgt aaactgtcgc tgacacaagc aggcgagcag
     5281 ctctaccaga agacggcatc tggatttgaa atgttagatc ttgggcttgc cacgctggcg
     5341 cactatcgag agacgccctc cggcacggtg cgtatcaatg ccagccagca cgcgattgat
     5401 aaatgcctgc tgccaaagct ggcggtattt aaacagcgat atcctgatat ccggcttgaa
     5461 ctgataaatg agagccggtt cgtcgatatc atcgaggaaa gattcgatgc aggtgtccgc
     5521 ctggggcctg aagtcggcca gggtatggtc gcggtacgca tcacgcccga tatggagatg
     5581 gcaattgtgg ggactccgga acattttcgt cgctatggtt ttccgcaaac cccggcggat
     5641 ttaaaggcgc atccctgtat cgcctatcag tttgccgacg gcagcgtata ccagtgggaa
     5701 ctcaatcagg atgataaaaa aatcactcat cgccctgaag ggcaatgggc cttatccgac
     5761 agctatatgg aagcagaagc agcccggctg ggcctcggat tggcctatgt tcctgttgaa
     5821 ctggtcgcgg atgatttaga acacggtaag cttatcaggg tgttgcagcg ttacagcctg
     5881 cgaatggagg gattgtttct ctattaccct catcgcaacg tctcccccgc cctgcggatg
     5941 gtcattgata cattgaaaat ctgaacgtta ataacgaccg actcttcact gtgcaaaatt
     6001 gtgtcaggta gtccctctgc gttcaattcc agggtctgct ttgagcgaga aagaggggac
     6061 tatccagcaa tggagatcac ccaaaagaaa aaaccacctg gctccctcga caaccttcat
     6121 ccgggcattg agcctcaatc aacattttta tatctgtcag gttacttcat tatgaaaact
     6181 gatcatcatc gttgtttttg ttgcaatagt agaatacgcg tattt
//
LOCUS       DAYPHN010000219         6200 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000219 DAYPHN010000000
VERSION     DAYPHN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.219"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1371)
                     /locus_tag="ACG0BW_004443"
     CDS             complement(<1..1371)
                     /locus_tag="ACG0BW_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261316.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRAWA"
     gene            1548..2339
                     /gene="kdgR"
                     /locus_tag="ACG0BW_004444"
     CDS             1548..2339
                     /gene="kdgR"
                     /locus_tag="ACG0BW_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFK7261317.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(2483..2721)
                     /locus_tag="ACG0BW_004445"
                     /pseudo
     CDS             complement(2483..2721)
                     /locus_tag="ACG0BW_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
     gene            2880..3023
                     /gene="mgrB"
                     /locus_tag="ACG0BW_004446"
     CDS             2880..3023
                     /gene="mgrB"
                     /locus_tag="ACG0BW_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFK7261318.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3098..3385
                     /locus_tag="ACG0BW_004447"
     CDS             3098..3385
                     /locus_tag="ACG0BW_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFK7261319.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            4055..4198
                     /locus_tag="ACG0BW_004448"
     CDS             4055..4198
                     /locus_tag="ACG0BW_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFK7261320.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            4211..4420
                     /gene="cspE"
                     /locus_tag="ACG0BW_004449"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             4211..4420
                     /gene="cspE"
                     /locus_tag="ACG0BW_004449"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFK7261321.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            4586..5395
                     /gene="rlmA"
                     /locus_tag="ACG0BW_004450"
     CDS             4586..5395
                     /gene="rlmA"
                     /locus_tag="ACG0BW_004450"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFK7261322.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(5392..5958)
                     /gene="mntP"
                     /locus_tag="ACG0BW_004451"
     CDS             complement(5392..5958)
                     /gene="mntP"
                     /locus_tag="ACG0BW_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFK7261323.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(6089..6179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT         1712 a         1409 c         1516 g         1563 t
ORIGIN      
        1 tgcccaggct cgtggctgag tgatacgtaa accactgaca caggcagcaa ttactgccag
       61 aatagccgca gccatcagcg agacgtgcgt accattatca ccaaactgat ttagcatcag
      121 cgccaccagc gccgcgccac tactctgacc caatagacga gccgtcccta acatgccact
      181 ggccccaccg ctacgttcgc gcggagcgga ggttataatg gtgtggttat tgggtgactg
      241 gaataagcca aatccagcgc cacataagat catcggccag ataatattga tatccgcagg
      301 tgacgcgggc agcagaacca gggaaaaaag ccccgcagcc atgatgaaca accctaaagc
      361 acccagcaat cctgcatgta cgcgttcaat caaatagcct gccagcggag ccatcaccat
      421 cgttgctaac ggccacggtg tcagaagtaa acctgtttcg acttcactac gcccgagcac
      481 ggtttgcagg taaaagggca gggaaaccat tgccagcatt tgtgcgcaga aagagcaaac
      541 agatgtgcaa atagaaagtg aaaacagcgg gatacgcagt aaatccaccg gtagcagcgg
      601 tacgggaaga gaaagctggc ggcgaataaa gaaaatacca acgacaacca ttaccaccag
      661 ttccgcacca atcaatgtca gcgattgccc ctgagcgaaa ccactcaacg cagtgataag
      721 caggccgaag gttaacgcgt tcatcacggc gctgggcagg tcgaaacggg gtttactggc
      781 gcgagaacca ttgggtggca gaaaacgcat tgccagaagc agggcgataa tacccaacgg
      841 tacgttgatt aaaaataacc atttccagga tgcgatggag aggattgctg cagcaattgt
      901 cggcccggca gcagaagaaa cggcaacaat aaacgagttt atgcccatcc ctctacccag
      961 aaaacgttgt ggatagatca ggcggataag tgcggtatta acgctcatca acgctgcacc
     1021 gccgaaacct tgtatgacac gcgcaagggt gagcatttgc agcgaatcag aaagggcgca
     1081 gaacagtgaa gacaacagaa aaacgaccag accgcattta taaatacgtc gatagccaaa
     1141 catatcgccc agaaacgaaa atgagagcag ggagatgaca atggcgattt gataggcgtt
     1201 cactacccag atggaactgg ctggcgtggc atgaaggtcc gtggcgattg ttggcagtgc
     1261 gacgttggcg attgcgccgt caaggacagc catcgaaata ccaatcacaa tggttaatat
     1321 cgcaccgtat cgctggggca atggcaggcc gtcggcctga acttttggca taggaatttt
     1381 atatctttgg tgaataatca acaggggtat gattctagca ctgatattca cggtggatgt
     1441 cgcagaattg taactaaccg gcaaaagatg gattgcgacc accctggtgc gaaattataa
     1501 taaaaaacag ttctgatttt tataaaacac tcgcaatgag gtgataaatg gctaacgcag
     1561 atctggataa acagcctgat tctgtatctt ccgtgctaaa agtttttggc attttgcagg
     1621 cgctgggtga agagcgcgaa atagggataa ccgagctgtc gcagcgcgtc atgatgtcaa
     1681 aaagcaccgt ttatcgcttt ttacagacca tgaaaaccct gggttatgtg gcgcaggaag
     1741 gggagtcgga gaaatattcc ctgaccctga aattgtttga actgggcgct cgcgcgttac
     1801 aaaacgtcga tttaattcgt agcgcagata tccagatgcg tgagatctcc cgcctgacca
     1861 aagaaactat ccacctcggc gcactggacg aagacagtat tgtttacatt cacaaaattg
     1921 actctatgta caatttgcgc atgtattcac ggattgggcg tcgtaatccg ctgtacagca
     1981 ccgcgattgg taaggtactg ctggcatggc gcgatcgcga tgaagtgaag caaattcttg
     2041 agggcgtgga gtataaacgc agtaccgaac ggaccatcat cagtacagaa gcgttattac
     2101 ccgttctgga ccaggtgcgc gagcaggggt atggcgaaga taacgaagag caggaagaag
     2161 gactgcgttg cattgcggtt ccggtatttg atcgctttgg cgtggtcatt gccggtctta
     2221 gcatctcctt cccgacgttg cgtttctctg aagagcgttt acaggaatat gtcgcaatgt
     2281 tgcataccgc ggcgcgcaaa atttctgccc aaatgggtta tcacgactat ccattctgat
     2341 gagagtaaga acctgtctga atatcaaaca gacaggttct ttatttagca tgagaaaaat
     2401 aaagttgaag gtggcgttat attaaacgca cttgcgataa gaatatttta ctcatgagtg
     2461 agaatctggt tatttattac ccttaaccat tatcgaccac aatattgctt ttacgtaaca
     2521 acgggcaatc tgttaaccca gaaaaccact tttagtgtgc aggtattgta ccgtgctggt
     2581 gcctctggcg gtcagatagg tacattgcaa acctaatccc gcggcattct ctttgcttcc
     2641 aatcaaaacg ccatatccgc tgagtaataa tcctatccat accagtgcta tcagcataac
     2701 tgtgcgaatg atgaatcgca ttacaacctc ttctcttttt atgttcgctt aatcgtagcg
     2761 gcaatatgcg ctgaagcaag cgactcattc tgaaaaagca cgaatatcga catagttagg
     2821 cgctgtttaa ctaacgcatg ctagtttaat gacataaggt aggtgaaacg gagattggag
     2881 tgaaaaagtt tcgatgggtc gttctggttg tcgtggtgtt ggcttgcttg ctgctttggg
     2941 cgcaggtatt caacatgatg tgcgatcagg atgtacaatt tttcagcgga atttgtgcca
     3001 ttaaccagtt tatcccgtgg tgatatcatt ttttcagcac tagattttct tcctctccag
     3061 cagtggtaga atagcgcctc tactcagagg tggtgaaatg aacgaagttg taaattcagg
     3121 cgtgatgaac attgcgtctt tggttgtatc ggtggtggtt cttcttatcg ggctcatctt
     3181 gtggtttttt atcaatcgtg ccagttcacg gactaacgaa cagattgaac tgcttgaggc
     3241 gttgctggat cagcaaaaac gtcaaaatgc tctgttacgt cgtttgtgcg aagcaaacga
     3301 acctgagaaa gcagataaaa agaccgttga gagtcaaaaa tcggttgaag acgaagatat
     3361 tattcgtctg gtcgccgaac gataatccta cctcaatctt gagtaacccc gtcgttgaag
     3421 ggttactcaa ttcatcctga ttatacgcac tgccatggtg caactgcttc aaattaaaac
     3481 tctggtctga cttcaaataa ttatcatatt atttatttgc gtaacgggat atcagaaaaa
     3541 gatatttagc ccattaattt attaggatgt ttacatcgga tttgtgatta agtgtggtat
     3601 tatttattag cgaaacgttt ctctcttgat ttttttgcgc attcatcaat ttttcttatt
     3661 ttaaatttac aatacctttg gcgattgact tctctttggg gtgattgata gtcgttaact
     3721 gactgtttta tgagaaaaag tgatataact ttttattcat tgcatagcag aaaatgtgat
     3781 attgcacgca ctatgtaata acttctccca ttggcctgga acaactgaac ttattgaact
     3841 atgttagaaa atacgtcagt ttaagtatct gcctgaactg gcaaggttaa gcacaatgat
     3901 atatcggcgc gtattccgtt gcataagtgt gcaaaaaaag tggaagacgt atcgagattt
     3961 gtgcgtctga tcgagacatg tttaaaaatg gcttgccata attaacgttg tatgtgataa
     4021 cagatttcgg gttaaacgag gtacagttct gtttatgtgt ggcattttca gtaaagaagt
     4081 cctgagtaaa cacgttgacg ttgaataccg cttctctgcc gagccttata ttggtgcctc
     4141 atgcagtaat gtgtcagttt tatctatgtt atgcctgcgg gcgaagaaaa caatctaagg
     4201 aatttttcaa atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt ctaaaggttt
     4261 tggcttcatt actccggctg atggcagcaa agacgtgttc gtacacttct ccgctatcca
     4321 gggtaatggc ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa ttcaggacgg
     4381 tcagaaaggt ccggcagctg ttaacgtaac agctatctga tcgaatccac tgatctgaag
     4441 tgtgaatacg cttcaatctc gctataaagc ctcgtcgaat gcgaggcttt ttactttgct
     4501 ttatcttcgc tccagtcgtt cggatatttg cccgccgcgt gattcgcgtt acacttgcgg
     4561 cctttagtat cctgccggag ttgtcatgtc gttttcctgt ccactttgcc atcagcctct
     4621 ttcgcgtgaa aaaaacagct atatctgtcc ccagcgacat cagtttgata tggcgaaaga
     4681 agggtatgtc aatctgctgc ctgttcagca taaacggtct cgtgatccgg gcgacagcgc
     4741 ggaaatgatg caagcacgcc gcgcattctt agatgccgga cattatcagc cgctgcgtga
     4801 tgcaattgtc gcccaactgc gggaacggct tgatgaaaag gccacagcgg tgctggatat
     4861 tggctgtggt gaagggtatt acacacacgc atttgccgat gcgctgcccg aaatcaccac
     4921 gtttggcctg gatgtctcga aggtagcgat aaaagcggcg gcgaaacgct atccgcaggt
     4981 cactttttgt gttgcttcca gccaccgttt gccgttttcc gataccagta tggacgccat
     5041 aatacgtatt tacgcgccgt gtaaagcaga agaattagcg cgagtagtga agcctggcgg
     5101 ttgggtcatt actgccacgc cgggaccgcg acatttgatg gagctgaagg ggctgattta
     5161 caatgaagta catcttcatg caccacatgc agaacaactg gaaggtttta cattacagca
     5221 gagtgatgag ttgtgttatc cgatgcgtct tcgcggtgat gaagccgtcg cattattgca
     5281 gatgacgccg tttgcctggc gtgcgaagcc agaagtctgg caaacactgg cagcaaaaga
     5341 agtgttcgac tgccagacgg actttaatat tcacctctgg cagcgttctt attaaccgtg
     5401 gaagtgcgtc cagaggatct ggacgccgat gccgatcagc accagcccgc cgagaatttc
     5461 cgcttttttc ccaataattg agccgataaa gcgaccaacc atcatcccta atgtcgacat
     5521 aatcaaggtt gcacaaccaa tggccaatgc ggtcgcgata atgttgacct gcaggaaagc
     5581 aagaccaaca cccacagcca tggcatccag gctggtggca atcgcggtgg ttaccagtag
     5641 ccagaaaccg tgtcgacggc gcggctcttc atcttcatca tctgcgccac gaaaaccctc
     5701 aataatcatt cgcccgccga ggaatatcag cagcacaaac gcaatccagt ggttccattc
     5761 aaggacaaac cggctggcta acatgcccat tccccagccg atcagcggcg tcagggtttc
     5821 gacagcacca aaaataaggc cggttcgcaa cgcttcagaa aatttcggtt tatggagggt
     5881 ggcaccttta ccgattgatg cagcaaatgc atccatcgac ataccaaacg caagaagaac
     5941 agtagcagtg atattcatga caacgtcctg accggggtat ccataaaaca catcatcgcc
     6001 cccagcaaac atgcatacat gaacgacaaa ccgcgatggt tttcgcgttc atgcaggcat
     6061 agcgattgat gtgcatatgg tctcgcctga ctgaaagtgt tcagtccgta cgtgccacgt
     6121 tttgcaacga gtatgttgac acgtacattt ccggaatctg gaaatcggct actccccaaa
     6181 tgagggcgca accttaacat
//
LOCUS       DAYPHN010000220         5998 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000220 DAYPHN010000000
VERSION     DAYPHN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.220"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(43..1038)
                     /gene="gapA"
                     /locus_tag="ACG0BW_004452"
     CDS             complement(43..1038)
                     /gene="gapA"
                     /locus_tag="ACG0BW_004452"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFK7261324.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1380..1793
                     /gene="msrB"
                     /locus_tag="ACG0BW_004453"
     CDS             1380..1793
                     /gene="msrB"
                     /locus_tag="ACG0BW_004453"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFK7261325.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1835..2107
                     /locus_tag="ACG0BW_004454"
     CDS             1835..2107
                     /locus_tag="ACG0BW_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFK7261326.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            2477..3553
                     /locus_tag="ACG0BW_004455"
     CDS             2477..3553
                     /locus_tag="ACG0BW_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFK7261327.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTDKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLNFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            3580..4959
                     /locus_tag="ACG0BW_004456"
     CDS             3580..4959
                     /locus_tag="ACG0BW_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFK7261328.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVSFGLLVCWSNAVGGL
                     ILAQLKELGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVVNK"
     gene            4976..>5998
                     /locus_tag="ACG0BW_004457"
     CDS             4976..>5998
                     /locus_tag="ACG0BW_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFK7261329.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDMMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATDIAVVDVLDKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEEMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYQDVQQAFEESVNN
                     KRDIIKG"
BASE COUNT         1527 a         1336 c         1540 g         1595 t
ORIGIN      
        1 gcgaccgaag tcgctctttt tagatcacag tgtcatctca acttatttgg agatgtgagc
       61 gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg ataccagttt
      121 cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag tgcaaacttc
      181 gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc ctttcatttc
      241 gccttcagca gcagctttaa cggcagcttt gatctgctcg taagttgcag ctttttccag
      301 acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg ccataccagt
      361 cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac cggtagagga
      421 cgggatgatg ttctgagaag cgccacggcc gccgcgccag tctttgtgag acgggccgtc
      481 aacggttttc tgagtagcgg tggtagcgtg aacggtggtc atcagacctt cgatgatacc
      541 gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc aggaagcgtt
      601 ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga acatcggagt
      661 gttgtcttta gacggaccag tcataaccac ttttttcgca ccagcggtga tgtgtttacg
      721 agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa caccaacttc
      781 gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt taccgttaac
      841 gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag tggagtcata
      901 tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa cgatctcgat
      961 gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa aaccgttgat
     1021 acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa ggttgcctgt
     1081 aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg cgacaaatca
     1141 atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg cttgagacca
     1201 catggggtcg gcgtcccgaa ttttaaaggg caattacgat aaaaatgtga ttttcatcac
     1261 gatttaggtg cagccatttc gtcgagatca agtttaggac aaatttttgc cagttggtgt
     1321 taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga tgtgagcaaa
     1381 tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg cagttctacg
     1441 tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat aacaagcgtg
     1501 acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa accaagtatg
     1561 attccggctg tggctggccc agtttctacg aaccggtgag tgaagaatcc attcgttata
     1621 tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac tgtgatgccc
     1681 atctggggca tgtcttcccc gacgggccgc agccaacggg tgaacgttat tgtgttaact
     1741 cagcctcttt acgctttacc gatggcgaaa acggcgaaga aatcaacggt tgaaaaaacg
     1801 attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca ttatcaacag
     1861 catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaattgggga aatggcctga
     1921 tggcgttgcg ttgaccgagg aacaaaaaga gaactgcctg caactggtga tgctgtggca
     1981 agcccgcaat aataccgaag cacagcatat gacaattgac acaaatggtc agatggtcat
     2041 gaagagcaaa caacagttaa aagaagactt tggtatctca gcaaagccta ttgccatgtt
     2101 taagtaagtg gtttgtcccc tttttgccgc ggttataaag gggatagtca acaataaccc
     2161 cacgactcat ctcaattatc tcgtcacgac caggcaaagc cccagcccgg tctcttgcag
     2221 tcgcgttcac gcctgaatga cttctcctga acttaagtct cagacctatt tggccggtaa
     2281 tccctctcaa atttgctcat tttgcgttcc gccttccatc aacctgtaac agatccagcc
     2341 attttctgcg taattgtgat ctgagttgta aaacgcagat cattatctgt gttttacaga
     2401 tttaatgttg acctcaaaaa tcatgtgggt aagtatgatg accatctaag caaaacaaaa
     2461 cacagaggat gacgcaatga aagcactggc tcggtttggc aaagcctttg gcggctacaa
     2521 gatgatcgat gtcccacaac ccatttgtgg cccggaagat gtagtgattg aaattaaagc
     2581 cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc gatagcggtt ctgatgagtt
     2641 taactctatc cgcggccatg agttcgcagg ttgtattgcg caggttggtg aaaaagtcaa
     2701 agactggaaa gtggggcaac gcgtcgtgtc agataacagc ggtcacgtct gcggcgtttg
     2761 tccggcctgt gaacaaggtg attttctgtg ttgtacagat aaagtaaacc ttggtctgga
     2821 taacaatacc tggggcggtg gtttttccaa atattgtctg gttcctggtg aaattctcaa
     2881 aattcatcgt catgcgttgt gggaaatccc tgatggtgtt gattatgagg acgcagccgt
     2941 acttgaccct atctgtaatg cctacaaatc catcgcgcag caatcgaaat tcctccctgg
     3001 tcaggatgta gtcgtcatcg gtactggccc actcgggctg ttctccgtac aaatggcgcg
     3061 gattatgggg gcggtaaata tcgtcgtcgt tggtctacaa gaagatgtgg cggtccgctt
     3121 cccggttgca aaagaactgg gtgcgacggc agtagtaaat ggttctaccg aagatgtggt
     3181 ggcgcgctgc cagcaaatat gtggcaaaga caatctgggg ctggtgattg aatgctccgg
     3241 tgccaatatc gcattgaaac aagccatcga aatgctccgc ccgaacgggg aagtggtacg
     3301 cgttggaatg ggcttcaaac ctcttaattt ctcgattaat gacattaccg cctggaacaa
     3361 aagcatcatt gggcatatgg cctatgactc cacctcatgg cgtaacgctc tccggctatt
     3421 agccagcggc gctatcaaag tcaaaccgat gatcacgcat cgtatcggcc tgtcgcaatg
     3481 gcgcgaaggg tttgatgcga tggtcgataa aaccgcaatc aaagtgatca tgacttacga
     3541 ctttgatgaa taactactct cctgaaggaa cgttatcgca tggaacagat aacaaaaccg
     3601 cattgtggtg cacggctgga tcgcttaccg gattgccgct ggcattcgtc aatgtttgcc
     3661 atcgtctcgt ttggcttgct tgtctgctgg agtaatgccg ttggtggctt gatcctcgcg
     3721 cagctgaaag agttgggctg gacagataat tccaccactg ccacattctc agcaatcacg
     3781 accgccggaa tgtttctcgg tgcgctggtt ggcggcatca tcggtgacaa aaccggtcgc
     3841 agaaatgcgt tcatcctcta tgaggccatt catattgcct cgatggtggt cggtgctttt
     3901 tcaccgaata tggatttcct tattgcctgc cgttttgtga tgggcgttgg gctgggagct
     3961 ttactggtga cgctgtttgc tggtttcacc gaatatatgc ccggtagaaa tcgcggaacg
     4021 tggtccagtc gggtttcttt tattggcaac tggtcatatc cgctctgttc attgattgcg
     4081 atgggactca cgccgctgat tagtgcagag tggaactggc gagtacaact gcttatccct
     4141 gcaatattgt cgcttatcgc tacggcgctg gcctggcgct actttcctga atccccgcgc
     4201 tggctggaat cgcgcggacg gtatcaggaa gccgagaaag tcatgcggag tatagaagaa
     4261 ggcgtcatac gccagacggg aaaacctttg ccgcccgtgg ttattgctga tgacggtaaa
     4321 gcgccacaag cggtgccgta ttcagcctta ctgacaggag tattactgaa acgcgtgata
     4381 ttaggttctt gtgtgctgat tgccatgaac gttgtgcagt acacactaat taactggttg
     4441 ccaacgatat tcatgaccca ggggattaat ttaaaagact cgattgtttt aaataccatg
     4501 agtatgtttg gcgcgccatt tggtattttt attgccatgc tggtgatgga taaaattccg
     4561 cgtaaaacaa tgggggtggg gctattaatc ctgattgcgg tgctcggata tatctattca
     4621 ctgcaaacca gtatgttgct cataacgctg attggtttct tcctgattac tttcgtctat
     4681 atgtacgttt gctatgcctc ggcagtgtat gtccctgaga tctggccgac agaggccaaa
     4741 ttacgtggct ccggtctggc gaatgcagta gggcgaatca gtggtattgc cgcaccttat
     4801 gccgttgcag tgctgctcag tagttatggc gtaacgggag tctttattct tctgggggcg
     4861 gtttcaatta ttgtcgcaat tgctatcgcc accattggaa ttgaaaccaa aggtgtctcc
     4921 gttgaaagtt taagtattga tgcagtagtc aataaataat actggtggat tcaaaatgaa
     4981 aaattcaaaa gcaatattgc aggtgccggg cacaatgaaa attatttcag cagaaatacc
     5041 tgtgcctaaa gaagatgaag ttttgattaa agtggaatat gtcggtattt gtggttcaga
     5101 tgtacatggt tttgaatcag gcccgtttat tccgcctaaa gacccaaatc aagaaattgg
     5161 cctgggtcat gaatgcgccg ggacggttgt ggctgtggga agccgagtgc gcaaatttaa
     5221 accgggggat cgagtaaata tcgaacctgg cgttccttgc ggtcactgtc gttactgtct
     5281 ggaaggcaaa tataacatct gcccggacgt tgattttatg gcgacacaac ctaactaccg
     5341 cggcgcatta acgcactatc tgtgtcatcc ggagagcttt acttacaaac tgcccgacaa
     5401 tatggacatg atggaagggg cgctggtgga gcctgctgca gtcgggatgc atgccgcgat
     5461 gctggcagat gttaaaccgg gtaagaagat aattattctg ggagcgggtt gtattggttt
     5521 gatgacgttg caagcgtgca aatgcctggg agctacggat attgccgtcg ttgatgtact
     5581 ggataaacgt ctgacaatgg cggaacagct cggcgcgaca gtggttatta acggtgcaaa
     5641 agaagacact attgcacgct gtcagcaatt taccgaagaa atgggcgcag atattgtttt
     5701 cgaaacagcg ggttctgcgg tcaccgttaa acaggcacct tatctggtaa tgcgcggcgg
     5761 taaaattatg attgttggta ctgtaccggg cgattcggca atcaatttcc tcaaaatcaa
     5821 tcgcgaagtc actattcaga cagtattccg ctatgccaat cgttatccgg tcacgattga
     5881 agctatttcc tcagggcgat tcgatgtgaa atcgatggtg acacatattt acgattatca
     5941 ggatgtacaa caggcatttg aagagtcggt taacaacaaa cgcgacatta ttaaaggc
//
LOCUS       DAYPHN010000221         5855 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000221 DAYPHN010000000
VERSION     DAYPHN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.221"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            228..1883
                     /gene="aslA"
                     /locus_tag="ACG0BW_004458"
     CDS             228..1883
                     /gene="aslA"
                     /locus_tag="ACG0BW_004458"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFK7261330.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1985..3220)
                     /locus_tag="ACG0BW_004459"
     CDS             complement(1985..3220)
                     /locus_tag="ACG0BW_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFK7261331.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGAEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3367..3443)
                     /locus_tag="ACG0BW_004460"
     tRNA            complement(3367..3443)
                     /locus_tag="ACG0BW_004460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3407..3409),aa:Pro,seq:tgg)
     gene            complement(3486..3572)
                     /locus_tag="ACG0BW_004461"
     tRNA            complement(3486..3572)
                     /locus_tag="ACG0BW_004461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3536..3538),aa:Leu,seq:cag)
     gene            complement(3593..3668)
                     /locus_tag="ACG0BW_004462"
     tRNA            complement(3593..3668)
                     /locus_tag="ACG0BW_004462"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3633..3635),aa:His,seq:gtg)
     gene            complement(3727..3803)
                     /locus_tag="ACG0BW_004463"
     tRNA            complement(3727..3803)
                     /locus_tag="ACG0BW_004463"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3767..3769),aa:Arg,seq:ccg)
     gene            complement(3906..5291)
                     /locus_tag="ACG0BW_004464"
     CDS             complement(3906..5291)
                     /locus_tag="ACG0BW_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFK7261332.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRNGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKIFGL
                     NRHGKAHKLEE"
     gene            complement(5482..>5855)
                     /locus_tag="ACG0BW_004465"
     CDS             complement(5482..>5855)
                     /locus_tag="ACG0BW_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFK7261333.1"
                     /translation="TEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASGAQIVTVA
                     MGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWLYRLLSQP
                     SRIKRQLRLLRYLRWHYTGNL"
BASE COUNT         1514 a         1601 c         1432 g         1308 t
ORIGIN      
        1 aaacagcatc aataatcaac gcgatataac atcaccgcct tacatatcac ctgcgccaga
       61 ggtaggattg aaaacgctct cctgattttc caattcattt tctggataaa taaataattt
      121 attttcatca ctattattta tgcaatcatc ctgtcaggga gagggatctc aattatcaat
      181 gcttaattac gtcatcattt tgacgacatg aaaccggagc attttgcatg gaattttcgt
      241 tttcacccaa acttcttgtt gtggctgtcg ccgccgctct tccactcatg gccaacgcag
      301 cagatacccc gtcaaccgcc accgcacgca aaggctttgc cggatacgat cacccaaacc
      361 agtatctggt taaaccggcg accactattg ccgacaacat gatgccggtg atgcagcatc
      421 ctgcacaaga caaagaaacc cagcagaagc tggcagaact tgagaaaaaa accggtaaaa
      481 aaccgaacgt ggttgttttc ttgctggatg atgtgggctg gatggatgtt ggctttaacg
      541 gcggcggcgt ggcggtgggt aaccctacac cagatatcga cgccgttgcc agccaggggc
      601 tgattttaac ttcggcgtat tctcaaccga gttcttcccc aacccgcgcc acgattctta
      661 ccgggcaata ctccatccac cacggcattc tgatgccgcc aatgtacggg caaccgggcg
      721 gtctgcaagg gttaaccact ctgccgcagt tgctgcacga tcagggctac gtcactcagg
      781 ccatcgggaa atggcatatg ggggaaaaca aagagtcgca gccgcagaac gttggctttg
      841 atgatttccg cggctttaac tcggtgtccg atatgtatac cgaatggcgt gatgttcatg
      901 tcaacccgga agtcgccctg agcccggatc gttctgaata catccaaaaa ctgccgttca
      961 gcaaagatga cgttcatgcc gtgcgcggcg gtgaacagga agccattgcc gacattacgc
     1021 cgaaatatat ggaagacctg gatcaacgct ggatggaata tggcgttaag ttcctcgaca
     1081 agatggcgaa gagcgataag cctttcttcc tctactacgg cactcgcggc tgtcacttcg
     1141 ataactaccc aaatgcgaaa tatgcgggta gctctccggc gcgcacctct tacggcgact
     1201 gcatggtgga gatgaacgat atcttcgcca atctgtataa agcactggag aaaaacggtc
     1261 agcttgataa cacgctgatt gtgttcacct ctgataacgg tccggaagcc gaagttccgc
     1321 cgcacggtcg cacaccgttc cgcggtgcga aaggttctac atgggaaggc ggcgttcgtg
     1381 tgccaacctt cgtttactgg aaaggcatga tccaaccgcg taaatctgac ggcattgtcg
     1441 atctggcgga tctcttccct acggcgctgg atctggcggg gcatcctggg gcgaaagtag
     1501 cgaatttagt accgaaaacc acctttatcg atggtgtgga ccagacatct ttcttcctgg
     1561 gaacaaatgg tcagtctaac cgtaaggccg agcactactt cctcaacggt aaactttctg
     1621 ctgtgcgtat ggatgagttc aagtatcacg tcctgatcca gcaaccgtac gcgtataccc
     1681 agagcgggta tcagggtgga ttcaccggca cagtaatgca aacggcggga tcgtcggtgt
     1741 ttaacctgta caccgatccg caggaaagcg actctatcgg cgtgcgccat atcccaatgg
     1801 gtgtaccgct acagaccgaa atgcatgcgt atatggagat cctgaaaaaa tatccaccac
     1861 gcgcgcagat taaatctgat taagccggcg ctaacgctac atcgggccac actgaccttg
     1921 ccggatgcgg cgtgaacgcc ttatccggca atcaaccgca ggcggccaca aaatggccgc
     1981 ttttttactt attcaccacc atcaaatgcg cctgcataat gtcgctggcc ggacgaccgt
     2041 gcgccagcaa atcagccatt gctttaagat atggcggtag atggcggaaa taacgctgat
     2101 acccggcaca caaataattc agccccggtt tgccgctggc atcgagcata aagcggtgtt
     2161 tcggacagcc tccccagcac gcttttaaca cgttgcaact gcgacactgc gccggtaact
     2221 gcttaaattt atcttcacca aacacctgct gttgcgggga atctaccatt tctgcaattg
     2281 tctgctgaag catattcccc agtcgatatt gcggataaac atagtgatcg caggcgtaaa
     2341 cgtcgccgtt gtgttcaaca atcaccgagc gcccacaggt tggctgatga tggcaaaccg
     2401 ctcctggcgc accgacaaaa ttggcaaacg cccattcgat attcatcacg aaaatcttgc
     2461 cgacgtcgcg tttgatccag tggtcgaata tcgccaccaa aaattcaccg aactcatcgg
     2521 ggcgtaccga ccattccgtc agttcgccct gaatatcgcc gggagcatgt agcttaagtc
     2581 cagcatgagc agccgtttca tcagccaggc gctcgaccac cggaataaac tggatgaact
     2641 cagcgcctgc atcacacaga aaatcataga cttgcaaagg ttgtaacgca ctggtgcgat
     2701 taacgcagac cagcacgtta tagtcgacat gatgtttttg caggagcgtc agggcacgca
     2761 tcaccagctt atgcgttggt ctgccgcctt tggtcacgcg atattgattg tggatctcag
     2821 ccggaccatc cagcgataac cctacaagaa aatgattttc tgccagaaat gcacaccatt
     2881 cgtcatcaag cagaacgccg ttagtctgga agctgttact tatcttcctg ccagcaccat
     2941 atttcgcctg tagcgccacg gcacggcggt aaaaatccag cccgagtaac gttggttcgc
     3001 cgccctgcca ggtaaaagcc acttcgtttt gggtttcgct ggcagcgata tagtgacgga
     3061 catacgcttc cagcgtgtca tcgtccatat gcgtgactgg cttttcgcgg taaagggatt
     3121 gtttttcgag ataaaaacag tagtcacagt tcagattaca atcggaaccg ctcggtttcg
     3181 ccatcacatg aaaagcacgc gttggaacct gttgcagcat ggtcgctcct tgcctgaagt
     3241 catccttcaa taagttaagg gcgaaatcat accgtggcgc aatgagttat tacgggcgtt
     3301 tattgcgaag ggaaagtgac gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt
     3361 caaaattggt cggcgagaga ggattcgaac ctccgaccca ctggtcccaa accagttgcg
     3421 ctaccaagct gcgctactcg ccgaaatact gctttttgaa tttttagttc aattctttaa
     3481 agtcgtggtg cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa
     3541 gctagcgcgt ctaccaattc cgccaccttc gcattgtcac aacttctaat aatggggtgg
     3601 ctaatgggat tcgaacccac gacaactgga atcacaatcc agggctctac caactgagct
     3661 atagccacca ctacaaatct tgttacgcgg tattactacc accgcagctc aagcgccggg
     3721 actaaatggc gcgcccgaca ggattcgaac ctgagacctc tgcctccgga gggcagcgct
     3781 ctatccagct gagctacggg cgcttagcgc cgttgcgggg gtggataata cggacttccc
     3841 acccctctgt ctagtccctt tttaaataaa ttgcgcgttt ggttacggtt tgtgcgtttt
     3901 gctgcttatt cctccagttt atgcgcttta ccgtggcgat taagcccaaa aattttataa
     3961 atcgccgtca ccgccagcat aaagatgatg ccgacaaaca gcgacatacg cgtatcttca
     4021 ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca ttgttacgta gttggcccac
     4081 gggaacagga ttgagcggaa cggatggctg gcaatcgccg ctttatgcgc acgacgaaaa
     4141 cgcagctggc ttatcaatat cacaaaccat ggcaccatcc ccggaagcac gctggcactg
     4201 tagacgtaga caaacacacg ctgcggattg ggaatgatgt agttcaggca tgagccaatt
     4261 aacaggatag caatagatac tgccacaccc gcaaccggta cgccgttacg ggaaactttc
     4321 gccatcgccg tcggtaactg acggtttttc gccagtgcgt agagcatacg tccgcaactg
     4381 tacatgccgc tgttacagcc agagagcgca gccgtcagca ccacaaagtt gataatgccc
     4441 gccgctgcgg taatgccgat tttggcgaaa gtcagtacga acgggctacc gttgctgcct
     4501 atttcattcc acgggaagat agtaacgata acgaaaatcg cgcctacgta gaaaatcaga
     4561 atcctccaca gcactttgcc tactgcactg cgcagcgtca cctgcggatt cttcgcttca
     4621 ccagcggtaa tgccaatcag ctccacgccc tggtaggacg ccaccacaat acacaatgcg
     4681 gtcaggaacc ctttccagcc ccccgcaaag aaaccgccat gctctgtgag gttgctaaaa
     4741 ccaatcgact gcccgccatt gccaaagcca aagaaaatca cgcccaggcc aatgacaatc
     4801 atcacgataa tcgtggtgac tttgatcatc gcgaaccaga actcgatttc gccgtacaac
     4861 cgcaccgccg ccagattcgc caacgccacc agcgccaccg cgatcaatgc gggtatccac
     4921 tgcgccatct ccgggaacca gaactggaca taaacgccaa tggcggtgat ttctgagatc
     4981 cccaccgcca tccacataaa ccagtaagac caggcggtga gatagccaaa gaacgggctc
     5041 atatagcgat gcgcataaac ggcgaacgaa ccggtaaccg gttcgaggaa taacatttcg
     5101 cccattgaac gcatgatgaa aaagacgaac agcccggcga tgatataggc caacaatacg
     5161 gatggcccgg cccatttcag ggtactggcg gcccccataa acaggccgac gccaatggtg
     5221 ccccccaggg cgatgagttc gatatgtcga gcttccagcc cacgctgtag ctctggtttg
     5281 ttatctgcca taaatcctcg tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt
     5341 atgggtacat cgagtgttgc gaatgttttc gtaattcagg agaaatggca aataaagcat
     5401 taaaaatttg aatgctttgt gtaataaaaa agcagacagg cgacggagtg accactccgt
     5461 cgctttacaa agagaggaaa atcagaggtt gccggtgtag tgccagcgta aataacgcag
     5521 caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc aggcggtaga gccactccag
     5581 ccccagcgtt tgccagattt tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaagt
     5641 cccgccaacg cccatataca gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat
     5701 ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc gctccgctgg catgaatgcg
     5761 ttcaaacagc gcctgacgct gctcgggttt aaaataacca tcctggctgc caacgatatt
     5821 cacattccac tggttgcgca gtttagcttc agttt
//
LOCUS       DAYPHN010000222         5624 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000222 DAYPHN010000000
VERSION     DAYPHN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.222"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            207..746
                     /locus_tag="ACG0BW_004466"
     CDS             207..746
                     /locus_tag="ACG0BW_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFK7261334.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDIDGTTLTLR
                     IEGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISMDQLGFLRIHDIQPVMQ"
     gene            1045..1929
                     /gene="tsx"
                     /locus_tag="ACG0BW_004467"
     CDS             1045..1929
                     /gene="tsx"
                     /locus_tag="ACG0BW_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFK7261335.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(1967..2314)
                     /gene="yajD"
                     /locus_tag="ACG0BW_004468"
     CDS             complement(1967..2314)
                     /gene="yajD"
                     /locus_tag="ACG0BW_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFK7261336.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2443..3414)
                     /gene="secF"
                     /locus_tag="ACG0BW_004469"
     CDS             complement(2443..3414)
                     /gene="secF"
                     /locus_tag="ACG0BW_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFK7261337.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3425..5272)
                     /gene="secD"
                     /locus_tag="ACG0BW_004470"
     CDS             complement(3425..5272)
                     /gene="secD"
                     /locus_tag="ACG0BW_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFK7261338.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5300..>5624)
                     /gene="yajC"
                     /locus_tag="ACG0BW_004471"
     CDS             complement(5300..>5624)
                     /gene="yajC"
                     /locus_tag="ACG0BW_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFK7261339.1"
                     /translation="FISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQKRTK
                     EHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVLPKG
                     TMKAL"
BASE COUNT         1430 a         1473 c         1355 g         1366 t
ORIGIN      
        1 acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca atgcatacgg
       61 cgtcctgacc aggtggttaa cagaaatcta ttttaccctg aactgacgat acaacaaagg
      121 cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct accattaggc
      181 taattgcgac gagaaaggga cgacctatga atactaacgt ttttcgactg ctcctgctgg
      241 gaagcctgtt tagccttagc gcctgtgtgc agcaaagcga agtgcggcag atgaaacaca
      301 gcgtcagcac actgaaccag gagatgacgc aactcaataa agaaacggta aaaatcactc
      361 agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcct ggagcgaaaa
      421 caccagcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg aatattacgc
      481 ctgatataga tggcaccacc ctgacgctac gtattgaggg cgagtcgaat gatcccttgc
      541 ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac aactttcagg
      601 aaatcaatgt gcagaatcaa ttgattaacg cccctgccag cgtcctcgcc cccagcgatg
      661 ttgatattcc gttacagtta aagggtattt ctatggatca gttaggcttt ttacgtatcc
      721 acgacattca acctgttatg cagtaaacgt atcgcggggc gattttgtgc gtcccgcaac
      781 atcttttccc gtcattttgt tactccgctt acatcacctg gattgatagt aaaagtttgc
      841 aacaagggtg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga acgcaatcga
      901 ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga tttttataat
      961 aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca ctcccgcaag
     1021 ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg tgcggtactg
     1081 gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca gtatctttcc
     1141 gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt cggaccgcag
     1201 atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg gttcgacttc
     1261 tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgcaaa aggtatctgg
     1321 aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga caagctgacc
     1381 aatactgacc ttagctttgg tccgttcaaa gagtggtact tcgcgaacaa ctacatttac
     1441 gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct gggtaccgat
     1501 atcgatactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata ccagtggcag
     1561 aactatggcg cagcgaatga aaacgagtgg gacggttacc gtttcaaaat taaatacttt
     1621 gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac caacttcgac
     1681 tggggttccg atttagggga tgacagcggt aatgcaatca acggtattaa gacccgtacc
     1741 aataactcca tcgcttccag ccatattctg gctctgaact acgatcactg gcactactct
     1801 gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga actgaacttc
     1861 ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt agtaggttac
     1921 aacttctaat tgtgagaatg ccggattcca tatccggcat tttttatcac ttcttcttgt
     1981 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
     2041 catcttcccc tgcgataacg gtcgtaccat actgatctgc ttcggtatat tttgaatgct
     2101 cgtgatcgtg gcaataaaga cacaataact cccagttact gccgtcttcc gggttattgg
     2161 tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttggaataa acaaactcgc
     2221 gagaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga tagccgcttt
     2281 ctaaccgcgc atagttttta gggatgatag ccatgaaaag ttacctggat gaaatgtagg
     2341 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
     2401 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattacggca gaattgacgg
     2461 ctgatccgcc ccttcttttt ccactttctg ctgcagcatg tgttcgcgct tcatacccag
     2521 tttcagagcc agcgccgatg ctacgtagat ggaagatgca gtaccgatgg aaacaccgat
     2581 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagaggtaca gcatcaggat
     2641 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
     2701 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcgc gaatacggtc
     2761 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
     2821 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
     2881 cgccagcgca ataacaaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac
     2941 gaggatagat agcagcgccg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc
     3001 cacgctcgga ccgacgaact caatacgctt cactgccgca ttctgattgg tggattcgtt
     3061 aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
     3121 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcatcggct cttcaaaacc
     3181 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
     3241 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
     3301 gatagcggcg attaacagca gaccagagat gccaaaagcc cagtagtccc agcgcataaa
     3361 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
     3421 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
     3481 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
     3541 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
     3601 tgttcgcatc gaagatggaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
     3661 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gataagtacg ttcgcatcga
     3721 ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagc gttgcgcctg
     3781 gcaacagcga cataatgccg acaatcaaga tcaagttggc aatcagagca ctggtcgcaa
     3841 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
     3901 ggcaagcctc cagcccctgc tcaatgttct gcatacccag ggtcgggcca atggtacgtt
     3961 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
     4021 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
     4081 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
     4141 tcttaccgct gtctttgtac tccacaaaca gggtcgccat cggtttgccg atgttgtcct
     4201 tggtgaagtt agacataatg ttaccaccag cgctatcgag cgagatgtta acctgcggct
     4261 ggttgtattc gtcctggctg gaagtggagt cagtgatatg gtcaccggtc agaattacgc
     4321 gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
     4381 cgcggccgga agccgctgcg gcctgatcaa cgttggtgtt taccagacgg aattccagcg
     4441 ttgccgtcgc acccagaatc tctttcgcac gcgcggtgtc ctgaatacct ggcagttcaa
     4501 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacacca agttggttta
     4561 cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcaggc
     4621 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg
     4681 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga
     4741 tgctcaggcc gtagttgttt tctttacgaa cggtggtata cgggatgccc ttttcgcgca
     4801 ggtcgctgcg caggctatcg atgttttgtt cctggagttt gccaagcgcg gtgtccatat
     4861 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
     4921 cgtgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
     4981 tgtcacccat aacgcccatt aatgcttcac gagcgcgcag ttgggtgtca gtggagtcga
     5041 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
     5101 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
     5161 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
     5221 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
     5281 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
     5341 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
     5401 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
     5461 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
     5521 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
     5581 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaa
//
LOCUS       DAYPHN010000223         5438 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000223 DAYPHN010000000
VERSION     DAYPHN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.223"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(112..1395)
                     /gene="aroA"
                     /locus_tag="ACG0BW_004472"
     CDS             complement(112..1395)
                     /gene="aroA"
                     /locus_tag="ACG0BW_004472"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFK7261340.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVEVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTAVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1466..2554)
                     /gene="serC"
                     /locus_tag="ACG0BW_004473"
     CDS             complement(1466..2554)
                     /gene="serC"
                     /locus_tag="ACG0BW_004473"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFK7261341.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMIEFERRHG"
     gene            complement(2753..3445)
                     /locus_tag="ACG0BW_004474"
     CDS             complement(2753..3445)
                     /locus_tag="ACG0BW_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFK7261342.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3575..5335
                     /gene="ycaO"
                     /locus_tag="ACG0BW_004475"
     CDS             3575..5335
                     /gene="ycaO"
                     /locus_tag="ACG0BW_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFK7261343.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1404 a         1386 c         1288 g         1360 t
ORIGIN      
        1 aattaacgct accattaata aaaatacccc acaaggtccg aaaaaaaacg ccggcaatgt
       61 gccgacgtct ttgaaaagaa aggattgtct atgttatcgc ccgttattca ttcagccggg
      121 ttgacttatc cgcgccaatt gctcgaaata gtctggaaaa gttttagcgg tacattttgg
      181 atcaagaatc gtcacagccg tatctgataa tgcgaccagc gagaaacaca tcgccatccg
      241 gtgatcattg tatgttgcga tctcggcaaa gttcagtttt tccggtggag tgatacgaat
      301 gtaatcgtgc ccctcttcta cttccgcacc aactttacgc agttctgttg ccatcgcaaa
      361 caggcgatcg gtctctttaa cacgccagtt atagatattg cgcagcgtgg tggtgccttt
      421 tgcaaataac gccgccgtgg caatggtcat cgccgcatca ggaatatggt tcatatccat
      481 atcaatagcg ttcagttcac cccgcgtgca ggaaatataa tcatcgcccc agcaaatggt
      541 cgcgcccatt ttttccagca catcagcaaa gcgaatatca ccctgcatac tgttgcgtcc
      601 aatgccggtc acttttacag taccgccttt gattgctgct gctgccagga agtaagaagc
      661 ggaagatgca tcgccttcga ccaaataagt acccggagac tgataagact gcccgccttt
      721 aacgacaaat tgttgatagt gctgattttc aatttcaaca ccaaacgtct tcatcagatt
      781 gagtgtgatg tcgatgtaag gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc
      841 ttccggcgca agaggcgcag tcattaacag tgcggtgagg aactggctgg aaacggagcc
      901 atcaacttca acgttgccgc cagtaaagcc gccctgtaaa cgcaatggtg gataattttc
      961 ttgttccagg taagtgatct tcgccccgcc caggcgcagc gcatccacca gatgaccaat
     1021 ggggcgttct ttcatacgcg gttcaccggt cagcacaata tcattgctac ccagacaaag
     1081 agctgccgcc agcggacgca ttgccgttcc ggcgttaccg aggaacaact ccagggcacc
     1141 ttctgcgtgt aatggaccgc cgttaccgat gatttcgcaa cgcgtacgat cagctgaaag
     1201 cgtatagctt acccctaacg ctgctaatgc attcagcata tggcgcacgt catcgctatc
     1261 cagcagattg gttaatactg ttttgccgtg tgctaatgcc gccagcaata aagcgcggtt
     1321 agaaacgctc ttggaaccgg gcagattaat agtgccatcg acacgagcga tgggttgtaa
     1381 cgtcagggat tccatgaact caactctcta caacagaaat aaaaacccca caggctggct
     1441 gtggggatta agcaaaattt cggcattagc cgtgacggcg ttcgaactca atcatgaagt
     1501 ctgtcagcgc tttaacgcct tccagcggca tggcgttata aatagacgcg cgcattccgc
     1561 cgaccacacg gtgacctttc agcgcatgaa ggccagcagc aaaagactct tcaaggaaca
     1621 atttgtcaag cgcactgtcc gccaactgga acggcacgtt catccgcgaa cggttagctt
     1681 tcgccacgtc attgcggtag aaatcgctgt tatcaatcac cccatacagc agttctgctt
     1741 tttgctgatt gattttatcc atttcagcta caccgccgtt cgctttcagc catttaaaga
     1801 ccagaccaga cagataccag gcaaatgtcg gcggcgtgtt aaacatggag ccgttatcgt
     1861 tgaggatgga ataatccaga atcgacggac acgcgatatt cgctttgccc agcaaatctt
     1921 cacgaacgat gacgattgtc aggcctgccg ggccgatatt tttctgcgcg ccagcgtaaa
     1981 tcacgccata acggctgacg tcaatcggac gggaaagaat ggttgaagag aagtcggcgg
     2041 cgaccaccac atctttgccg aagtctggcg tttcgtcgat agcgataccg tcgatggttt
     2101 cattcgggca gtaatgcata taagcagcat tatcagagag ttgccattcg cgcattggct
     2161 taaccgcgcg cagaccatca acagtcactt tggcgtcgaa gacattaggc gtgcaatatt
     2221 ttttcgcttc tttaatggca cttgccgccc agtaaccggc atcaacataa tctgcggtgg
     2281 ttttatcacc gagaatattc agcggaacag cagcaaactg accgcgaccg ccgccatggc
     2341 agaacaatac cttgtagttg gaggggacat taagaagatc gcgaaaatcc ttctcggctt
     2401 cctctgcaac ctgaatgaac tctttgccac ggtgactcac ttccatcacc gacgtaccaa
     2461 gaccgttcca gtcgcgcagt tcctgttgag cctgtttaag cacctctgcc ggtagcattg
     2521 ccggaccaga actaaaattg aagatttgag ccatttcccc tcaccacgtt gcgttgctat
     2581 taacctgcgg tcaatcgatt gcgaccacag gtaatacatt attcctgtgg ctatcggttt
     2641 tatcattcag tgacacacat cgcaatgagt aaaaccgttg agggcaaaaa tgtggcttgc
     2701 atcacacaaa agaatctctc ggattattag caaaaagccg atgtttttac tgtcacgtca
     2761 cccgttttgc ccgacttgcc ttcgtccatt ctggatttgc acagcgcgga cataattgtt
     2821 tttcccccgc gttcatatga atgatttcac tgcaacgaat gcaggcatag tccgccgact
     2881 cgggtaccac tttgtaacgc tgagtacagt cactgggtaa aataagtaag cccggtttca
     2941 ccttgtcatc ttcaaagaaa tagacactga tttgcccgtt ggtttcgaga atcgccagac
     3001 gtacttgccc caactgctct acaccgcgca atcgtagctc cataaagaac tcaaattccg
     3061 tcatgttgga gttattgagt ttcgaccagg ccagctcgcc atcttcaata atgacaaccg
     3121 gcttaccttc cagaagatct tccagttttt cactgtgtgc catcaaccac attaccaggc
     3181 ggtataacaa cgccagagta ataaagacga taagtaccgg gaccatcggc acatcatcat
     3241 aaaacgccac atctcccgcc gctgatccca gcgtcagaat gattaacact tcaaaaagcg
     3301 acatctgccg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc agtacaaagg
     3361 tataaagact acgtagtgcc acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3421 tacggtggag atcaaacgcc ttcattttta tgctcttatc gtggtttatc cactaaaagc
     3481 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3541 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3601 agatgccgca ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3661 tcagattgaa gaggcctcct ggctgaatcc ggtgcctaac gtctggtctg tacatattcg
     3721 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaaa aagcggcgct
     3781 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3841 ctggctgggc gaaaccatcg ccaacggtcc gttcgtgcat taccccaacg aaaaatggtt
     3901 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3961 ttacgatccg gagaatgaac tggccggcag tatgctgatt gacctgcaat ccggtaacga
     4021 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     4081 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4141 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4201 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4261 ggtggttgaa gccatcgaaa cgctggaagc agaaggtttc ccaatctttg cttatgatgg
     4321 ttcgcttggc ggccagtatc cggtgatttg cgtggtactg ttcaatcctg ctaacggcac
     4381 ctgctttgcc tctttcggtg cacatcctga ttttggcgta gcactggaac gtaccgtgac
     4441 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4501 tgatgaagaa gttgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4561 aatctcctgg gatctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4621 cggcactacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4681 agtttatatt gccgattacg agcatctggg cgtttatgca tgcagaatta tcgtacctgg
     4741 catgtccgat atttatccgg ctgaagatct gtggctggcg aataacagta tgggcagcca
     4801 tttacgtgaa acgattcttt cgttaccagg cagcgagtgg gaaaaagaag attacctgaa
     4861 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4921 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4981 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     5041 ggagtttaac tcatcggtat tcagcccgga acgcgccaac tattatcgct gcctgcaaac
     5101 gttgttatta ctggcgcagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5161 tcgcatgtac ggcgcagatg ccgtggaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5221 gttttatggt ttgcaaccag tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5281 gttgaaggcc tacgaaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5341 aatagcctac gcaatgtagg cttaatgatt cgtctgagtt atattacggg gcgttttttt
     5401 aatgccccgc tttacatata tttgcattaa taaaataa
//
LOCUS       DAYPHN010000224         5403 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000224 DAYPHN010000000
VERSION     DAYPHN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.224"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            257..1591
                     /gene="cadB"
                     /locus_tag="ACG0BW_004476"
     CDS             257..1591
                     /gene="cadB"
                     /locus_tag="ACG0BW_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFK7261344.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1671..3818
                     /gene="cadA"
                     /locus_tag="ACG0BW_004477"
     CDS             1671..3818
                     /gene="cadA"
                     /locus_tag="ACG0BW_004477"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFK7261345.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3877..5334
                     /gene="dtpC"
                     /locus_tag="ACG0BW_004478"
     CDS             3877..5334
                     /gene="dtpC"
                     /locus_tag="ACG0BW_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFK7261346.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNTTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLVSPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFAARFLFSTPTNMIQESND"
BASE COUNT         1282 a         1290 c         1308 g         1523 t
ORIGIN      
        1 taaatttaac cagaaaatat cccgcaatcc attgtaaaca ttgaatgttt atcttttcat
       61 gatatcaact tgcgatcctg atgtgttaat aaaaaacctc aagttctcac ttacagaaac
      121 ttttgtgtta tttcacctaa tctttaggat taatcctttt ttcgtgagta ttcttagcgc
      181 cagtttggtc tggtcaggaa atagttatac atcatgaccc ggactccaaa ttcaaaaatg
      241 aaattaggag aagagcatga gttctgccaa gaagatcggg ctatttgcct gtaccggtgt
      301 tgttgccggt aatatgatgg ggagcggtat tgcattatta cctgcgaacc tagcaagtat
      361 cggtggtatt gctatctggg gttggattat ctctattatt ggtgcaatgt cgctggcgta
      421 tgtatatgcc cgactggcaa caaaaaaccc gcaacaaggt ggcccaattg cttatgccgg
      481 agaaatttcc cctgcatttg gttttcagac aggtgttctt tattaccatg ctaactggat
      541 tggtaacctg gcgattggta ttaccgctgt atcttatctt tccaccttct tcccagtatt
      601 aaatgatcct gttccggcgg gtatcgcctg tattgctatc gtctgggtat ttacctttgt
      661 aaatatgctc ggcggtacct gggtaagccg tttaaccact attggtctgg tgctggttct
      721 tattcctgtg gtaatgacag ctattgttgg ctggcattgg tttgatgctg cgacttatgc
      781 tgctaactgg aatactgcgg ataccaccga tggtcatgcg atcattaaaa gtattctgct
      841 ctgcctgtgg gccttcgtgg gtgttgaatc cgcagcagta agtactggta tggttaaaaa
      901 cccgaaacgt accgttccgc tggcaaccat gctgggtact ggtttagcag gtattgttta
      961 catcgctgcg actcaggtgc tttccggtat gtatccgtct tctgtaatgg cagcttccgg
     1021 tgctccgttt gcaatcagtg cttcaactat cctcggtaac tgggctgcgc cgctggtttc
     1081 tgcattcacc gcctttgcgt gcctgacttc tctgggctcc tggatgatgt tggtaggcca
     1141 ggcaggtgta cgtgccgcta acgacggtaa cttcccgaaa gtttatggtg aagtcgacag
     1201 caacggtatt ccgaaaaaag gtctgctgct ggctgcagtg aaaatgactg ccctgatgat
     1261 cctcatcact ctgatgaact ctgccggtgg taaagcatct gacctgttcg gtgaactgac
     1321 cggtatcgca gtactgctga ctatgctgcc gtacttctac tcttgcgttg acctgattcg
     1381 ttttgaaggc gttaacatcc gcaactttgt cagcctgatc tgctctgtac tgggttgcgt
     1441 gttctgcttc atcgcgctga tgggcgcaag ctccttcgag ctggcaggta ccttcatcgt
     1501 cagcctgatt atcctgatgt tctacgctcg caaaatgcac gagcgccaga gccactcaat
     1561 ggataaccac acagcgtcta acgcacatta attaaaagta ttttccgagg ctcctccttt
     1621 cattttgtcc catgtgttgg gaggggcctt ttttacctgg agatatgact atgaacgtta
     1681 ttgcaatatt gaatcacatg ggggtttatt ttaaagaaga acccatccgt gaacttcatc
     1741 gcgcgcttga acgtctgaac ttccagattg tttacccgaa cgaccgtgac gacttattaa
     1801 aactgatcga aaacaatgcg cgtctgtgcg gcgttatttt tgactgggat aaatataatc
     1861 tcgagctgtg cgaagaaatt agcaaaatga acgagaacct gccgttgtac gcgttcgcta
     1921 atacgtattc cactctcgat gtaagcctga atgacctgcg tttacagatt agcttctttg
     1981 aatatgcgct gggtgctgct gaagatattg ctaacaagat caagcagacc actgacgaat
     2041 atatcaacac tattctgcct ccgctgacca aagcactgtt taaatatgtt cgtgaaggta
     2101 aatatacttt ctgtactcct ggtcacatgg gcggtactgc attccagaaa agcccagtag
     2161 gtagcctgtt ctatgatttc tttggtccga acaccatgaa atctgatatt tccatttcag
     2221 tatctgaact gggttctctg ctggatcaca gtggtccaca caaagaagca gaacagtata
     2281 tcgctcgcgt ctttaacgca gaccgcagct acatggtgac caacggtact tccactgcga
     2341 acaaaattgt tggtatgtac tcagctccgg caggcagcac cattctgatt gaccgtaact
     2401 gccacaaatc gctgacccac ctgatgatga tgagcgatgt tacgccaatc tatttccgcc
     2461 cgacccgtaa cgcttacggt attcttggtg gtatcccaca gagtgaattc cagcacgcta
     2521 ccattgctaa gcgcgtgaaa gaaacaccaa acgcaacctg gccggtacat gctgtaatta
     2581 ctaactctac ctatgatggt ctgctgtaca acaccgactt catcaagaaa acactggatg
     2641 tcaaatccat ccactttgac tccgcgtggg tgccttacac caacttctca ccgatttacg
     2701 aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa agtgatttac gaaacccagt
     2761 ccactcacaa actgctggcg gcattctctc aggcttccat gatccacgtt aaaggtgacg
     2821 taaacgaaga aacctttaac gaagcctaca tgatgcacac caccacttct ccgcactacg
     2881 gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa aggtaatgct ggtaagcgtc
     2941 tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa agagatcaaa cgcctgagaa
     3001 cggaatctga tggctggttc tttgatgtat ggcagccgga tcatatcgat acgactgaat
     3061 gctggccgct gcgttctgac agcacctggc acggcttcaa aaatatcgat aacgagcaca
     3121 tgtatcttga cccgatcaaa gtcaccctgc tgactccggg gatggaaaaa gacggcacca
     3181 tgagcgactt tggtattccg gccagcatcg tggcgaaata cctcgacgaa catggcatcg
     3241 ttgttgagaa aaccggtccg tataacctgc tgttcctgtt cagcatcggt atcgataaga
     3301 ccaaagcact gagcctgctg cgtgctctga ctgacttcaa acgtgcgttc gacctgaacc
     3361 tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga tcctgaattc tatgaaaaca
     3421 tgcgtattca ggaactggct cagaatatcc acaaactgat tgttcaccac aatctgccgg
     3481 atctgatgta tcgcgcattt gaagtgctgc cgacgatggt aatgactccg tatgctgcgt
     3541 tccagaaaga gctgcacggt atgaccgaag aagtttacct cgacgaaatg gtaggtcgta
     3601 ttaacgccaa tatgatcctt ccgtatccgc cgggagttcc tctggtaatg ccgggtgaaa
     3661 tgatcaccga agaaagccgt ccggttctgg agttcctgca gatgctgtgt gaaatcggcg
     3721 ctcactatcc gggctttgaa accgatattc acggtgcata ccgtcaggct gatggccgct
     3781 ataccgttaa ggtattgaaa gaagaaagca aaaaataatt agctcgtaca agggaagtgg
     3841 cttgccactt cccttttttg ctcataagga gaacacatga aaacaccctc acagccgcgc
     3901 gcgatatact atatcgtggc gatccaaatc tgggagtact tcagttttta cggcatgcgt
     3961 gccttactca ttctctatct cacccatcag cttggttttg atgataacca tgccatcagc
     4021 ctgttcagcg catatgcttc tctggtttac gttaccccta ttctcggcgg ctggcttgcc
     4081 gaccgcctgc tcggcaaccg cactgcagtg attgccggcg cgctgttaat gacccttggc
     4141 catgtggtgc tgggcattga tacaaataca acctttagcc tgtatctggc gctggcaatc
     4201 attatttgtg gctacggttt attcaaatca aacatcagct gtttgcttgg cgagctctac
     4261 gacgagaacg atcatcgacg tgatggcggt ttttcgctgc tgtatgctgc gggcaatatc
     4321 ggttctatcg ccgcccccat cgcctgcggc ctggctgctc agtggtatgg atggcatgtt
     4381 ggctttgccc ttgcgggtgg cggcatgttt atcggtttgt tgattttctt aagcggtcat
     4441 cgtcatttcc agtccacgcg tagtatggat aaaaaagcgc tcactagcgt taaatttgcc
     4501 ttaccggtat ggagctggtt agtggtgatg ctctgtttag ccccagtatt ttttaccctg
     4561 ctgctggaga acgactggtc cggatatttg ctggcgatcg tttgccttat tgccgcacaa
     4621 atcattgccc gcatgatgat caaattcccc gaacaccgcc gtgctctttg gcaaattgta
     4681 ttgttgatgt ttgtcgggac gttgttctgg gtactggcac aacagggcgg cagtaccatc
     4741 agcttgttta tcgatcgctt tgtgaatcgt caggcattca atattgaagt acctactgca
     4801 ctattccagt cggtgaacgc aattgcggtg atgctcgctg gggttgttct ggcctggctg
     4861 gtgtcgccag aaagtcacgg caactcaaca ttgcgcgtct ggctgaagtt tgcctttggc
     4921 ttactactga tggcttgtgg ctttatgttg ttggcatttg atgcccgaca tgcagcagct
     4981 gacggtcaag cgtcaatggg cgtgatggta tccgggctgg cgctaatggg ctttgccgaa
     5041 ctctttattg acccggtggc gattgcgcaa atcacgcgtc tgaaaatgtc tggcgtatta
     5101 accgggattt atatgctggc aacaggcgcg gtcgccaact ggctggcagg cgtagtggca
     5161 cagcagacga cagagtcgca aattagcggt atggcaattg cagcttacca gcgattcttt
     5221 tctcaaatgg gagagtggac gttggcttgt gtcgcgatca tcgtcgtatt ggcctttgct
     5281 gcccgttttc tgtttagcac gccgacgaac atgatacagg agagcaacga ttaactgtcg
     5341 taaaaacaat tggttatgaa taaattaact ttcagtttat cacactagta aattgagatt
     5401 aat
//
LOCUS       DAYPHN010000225         5232 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000225 DAYPHN010000000
VERSION     DAYPHN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.225"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(66..461)
                     /gene="caiF"
                     /locus_tag="ACG0BW_004479"
     CDS             complement(66..461)
                     /gene="caiF"
                     /locus_tag="ACG0BW_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFK7261347.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(722..3940)
                     /gene="carB"
                     /locus_tag="ACG0BW_004480"
     CDS             complement(722..3940)
                     /gene="carB"
                     /locus_tag="ACG0BW_004480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFK7261348.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECESNPSDREKIMVLGG
                     GPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVE
                     RLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTA
                     VSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAY
                     TLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKAT
                     GVPLAKVAARVMAGKTLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTG
                     EVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDA
                     THGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRR
                     SALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3958..5106)
                     /gene="carA"
                     /locus_tag="ACG0BW_004481"
     CDS             complement(3958..5106)
                     /gene="carA"
                     /locus_tag="ACG0BW_004481"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFK7261349.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAVLALEKARAFPGLNGMDLAK
                     EVTTAETYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1150 a         1465 c         1338 g         1279 t
ORIGIN      
        1 tttggggtgc agttcagcca gcatcgcatc cggcgatcag gtcgcacggt atcaacgaaa
       61 tgacattaac gacgcatact ctttgacaac atcatctgcc acttctgctc gcggttaagt
      121 tccgtgagcg gaacggcagg aatacgcggc gtctttctta cgccgactaa tttttcccga
      181 ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt aaccagtcgc
      241 tgacactggc atccccgccc ttccagctta ttagggatca tcttaacttc gcagcttatt
      301 tctgtgactt ccgacagaat ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg
      361 aaatgaatag agatctctct tgctatcacc caccgatttt cagccatcat ccattcggcg
      421 attaacaagt agagtggttt ttcaacatat ccttcacaca tacgaactcc agtttcacaa
      481 attaaaagtc tggctatgac tcgttttgaa gcaattaaac aaggccatat taaaattgtg
      541 gatatatcga tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc
      601 atcaaaaata tcgattcccc aaaagattta cttaatgggc aaaaaaaacg gtcctcatca
      661 gagaaccgta ttagttattc gatcaaatta gcggatgaaa aatatccgcc atgacacgct
      721 attatttgat ctgcgcgtgc atttcctgca ccgaaattac tttttcagtc gcatcggcat
      781 tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc actttatatt
      841 gcagcgcact gcgacggatc acgcgggagt cttcaatcgc acgacggcct gaggtggtgt
      901 tgatgatgta ggtatattcg ccattcttga tacggtcctg aatgtgcgga cgaccttcat
      961 gcaccttgtt caccagacgc ggattaatgc ctgcttcgcc cagcacaatc gccgtgccgt
     1021 gggtcgcatc cagctcgaag ccctgtttca gcagttttgc cgccaggtcc accacgcgtt
     1081 ctttgtcgcc ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc
     1141 tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg gcccacgccc atgacttccc
     1201 cggtagagcg catttctggc cctaacagcg ggtcaacgcc cgggaattta ttgaacggca
     1261 gcaccacttc tttcaccgag tagtacggag ggataacttc tttggttacg ccctgctcgg
     1321 ccagcgtttt gcctgccatc acgcgcgccg ccactttcgc cagcggtacg ccggtggctt
     1381 tggagacgaa cggaacggta cgcgctgcac gcgggttaac ttcaatcagg tagacttcgt
     1441 tgtttttcac cgcaaactgc acgttcatca ggccgcgtac ctgcaattcg aaggccagtt
     1501 tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtag gctggcagcg
     1561 aacatgcgga gtcaccggag tgcacgcccg cctgctcaat atgctccatg atgccgccaa
     1621 tcagcaccat ttcgccgtcg cagatagcat ccacgtcaac ttctactgcg tcatcaagga
     1681 agtggtccag caacactggc gcatcgttag atacgctgac cgctgtctgg aagtagcgac
     1741 gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc gccgagaacg taagacggac
     1801 gcaccaccag cgggtagcca atctctttcg ctttctcaac cgccatttcg atggtggtaa
     1861 cggtggcgtt cgccggttgt ttcagtttca gacgctcaac cgcatgctgg aagcgttcac
     1921 ggtcttccgc acggtcgatg gcatccgggc tggtaccgat aaccggtacg ccagcagctt
     1981 ccagcgcgcg cgccagtttc agcggggtct gaccgccgta ctggacgata acgcctttcg
     2041 gcttctcaat acgcacaatt tccagcacgt cttccagagt taccggctcg aagtagaggc
     2101 ggtcggaagt gtcgtaatca gtggagacgg tttccgggtt acagttaacc ataatggttt
     2161 cgtaaccgtc ttcgcgcagc gccagtgagg cgtgtacgca acagtagtcg aattcgatac
     2221 cctgaccgat acggttcggg ccgccgccga ggaccatgat tttttcacgg tcagatgggt
     2281 tggattcgca ctcttcttca taagtggagt acatataagc ggtgtcggta gcgaactctg
     2341 ccgcacaggt atccacgcgc ttgtaaaccg ggtgcaggtc atactggtca cgcagcttac
     2401 ggatttctgc ttcacgcacg cccgcgagtt ttgccagacg cgcgtcggca aagcctttgc
     2461 gtttcaactg gcgcaggaag tcagcgttca ggccagtgat gcccacttcc gccactttct
     2521 cttccagacg caccagctct tcaatctgta ccaggaacca gcggtcaatg ttggtcaggt
     2581 tgaagacgcc gtctacggac aggcccgcgc ggaaagcatc ggcgatatac cagatacgct
     2641 ctgcgccagc gtctttcagc tcgcggcgga ttttagtcag cgcttccggg tcgtcgagac
     2701 tcactttcgg atcgaagccg gtcgcgccca cttccaggcc gcgcagcgct ttttgcaggg
     2761 attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc tgagtggtca
     2821 gacggtcgtt cgccccggcg aatttctcga agttgaagcg aggaattttg gtgaccacgt
     2881 agtcgatgga cggctcgaag gaggccggag tacgcccgcc ggtgatgtcg ttcatcagct
     2941 cgtcgagggt gtaacccacc gccagtttcg ccgccacttt ggcaatcggg aaaccagtgg
     3001 ctttcgacgc cagcgccgaa gaacgcgaca cgcgcgggtt catttcgata acaatcaggc
     3061 gaccgttttt cgggttcacc gcaaactgga cgttggaacc accggtttca acgccgattt
     3121 cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtcg gtcagcgttt
     3181 gggctggtgc gacagtgatg gagtcaccgg tgtggatgcc cattgcatcg aagttttcga
     3241 tagagcagac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc tcgtactctt
     3301 tccagccgat cagcgactca tcaatcagca gttctttggt cggagagaga tccagaccgc
     3361 gggcgcaaat ttcttcaaac tcttcgcggt tataagcgat accgccaccg ctaccaccca
     3421 tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaacc gccagcgctt
     3481 cttccatcgt gtgtgcgata ccggaacgcg cggtttccag accaattttc ttcatcgcta
     3541 cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcagtggca ccaatcatgg
     3601 tgacgccgaa ctcttccaac acgccctgac gttccagctc cagcgcacag ttcagcgccg
     3661 tctgaccgcc catcgttggc agcaccgcgt ccgggcgctc tttttcaata atcttgcgta
     3721 ccacttccca gtgaatcggc tcgatgtagg tcgcatcagc catttccggg tcggtcatga
     3781 tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcacgc agggctttac
     3841 acgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc gggcccgcac
     3901 ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta ctcctgatta
     3961 cttagcggtt ttacggtact gctcaattaa ctcgataaag tggtcgaaca acggcgctgc
     4021 gtcgtgtggc ccagggctgg cttcagggtg accctggaag ctgaacgccg gtttatcggt
     4081 gcgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac gcaggtttgc
     4141 aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt
     4201 tttctccaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca ttttgacagt
     4261 cttcgcaccg ctcgccagcg ccagcagctg atgaccgaga cagatgccga agaccggaat
     4321 atcggtttcg aggaatttct ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc
     4381 aggaccgttg gagaggaaga tgccgtctgg attcattttc aacacatctt ccgcagaagt
     4441 ttgcgccgga acgatggtca ggcggcagcc tctatccacc aacatgcgca ggatgttgcg
     4501 cttggcgcca aaatcataag ccacaacatg gaacggcagc tcgtcttctt tcttcgcttc
     4561 tggcaggccg ccggtcagcg tccagctccc ttgtgtccag ctatacgttt ctgcggtggt
     4621 cacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt tttctaatgc
     4681 cagcaccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg
     4741 cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt tatggcgctt
     4801 caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg gcaggtcgcg
     4861 aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat tagtgccgac
     4921 attaccaata tggggataag taagagtaac gatttgacga gaataggaag gatcagtgag
     4981 gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt
     5041 tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata gcgctgactt
     5101 aatcaaaaca ccctccagag aatattcact cactttattt gcatattaat tcacaatgat
     5161 gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg gcattctgga
     5221 gatatgggcg ct
//
LOCUS       DAYPHN010000226         5210 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000226 DAYPHN010000000
VERSION     DAYPHN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.226"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(211..1314)
                     /gene="livJ"
                     /locus_tag="ACG0BW_004482"
     CDS             complement(211..1314)
                     /gene="livJ"
                     /locus_tag="ACG0BW_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFK7261350.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILDKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(1586..2440)
                     /gene="rpoH"
                     /locus_tag="ACG0BW_004483"
     CDS             complement(1586..2440)
                     /gene="rpoH"
                     /locus_tag="ACG0BW_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFK7261351.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(2685..3743)
                     /gene="ftsX"
                     /locus_tag="ACG0BW_004484"
     CDS             complement(2685..3743)
                     /gene="ftsX"
                     /locus_tag="ACG0BW_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFK7261352.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(3736..4404)
                     /gene="ftsE"
                     /locus_tag="ACG0BW_004485"
     CDS             complement(3736..4404)
                     /gene="ftsE"
                     /locus_tag="ACG0BW_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFK7261353.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(4407..>5210)
                     /gene="ftsY"
                     /locus_tag="ACG0BW_004486"
     CDS             complement(4407..>5210)
                     /gene="ftsY"
                     /locus_tag="ACG0BW_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFK7261354.1"
                     /translation="LEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEE
                     MGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTF
                     RAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNK
                     SHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKL
                     DGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
BASE COUNT         1195 a         1491 c         1264 g         1260 t
ORIGIN      
        1 agaagcgtta atcgtcaagt ttgatttcaa aagtgacgac caacgagaaa ttgcctgatg
       61 cgctacgctt atcaggccta caatgtgtgc tgcaatttac tgatttcttt ggatcttgta
      121 ggccggataa ggcgtatacg ccgcatccgg catgaagcaa cgtactcgat attagcaatt
      181 tggcggcaac tccaagttgc cgattaatga ttacttcgca tcggtcgccg tgccgttggc
      241 gtgccagtca aatacgccga actcaaagcc tttcagatcg cctttctcat cccaggtcag
      301 cggccccatc accgtatcca cggagttcgc tttcaggtat ttggcgattt cagccggatc
      361 gtcagactga ttcagacccg cctgcaaaga ttgcagcgcg gcgtaggtgg tccagacgaa
      421 cgcgccgctt gggtcctgtt tcttcgcttt gatcgcgtca acaattggtt tgttcgccgg
      481 aacctgatcg tagttcttcg gcttggtcac cagcagccct tccgctgatt cgcccgcaat
      541 gttagacagc gagacgttag ccacgccttc cggccccata aactgagttt tcagccccgc
      601 cgcgcgtgcc tgacgcagga tttgccccat ttccgggtga taaccgccgt agtaaacgaa
      661 gtcgatattc tcttttttca gacgcgccac cagcgtagag aaatcttttt ccccggcggt
      721 gatgccatca aagaacacca cgttagcatt gcctttcttc aggccgtcct gcaccgcacg
      781 cgccagacct tcgccgtatt gctgtttgtc gtggacgata gcaatacgct gcggtttcac
      841 tttatcaaga atatatttcg ccgccgtcgg cccctggtcg gagtccaggc cggtagtgcg
      901 caggatcagc tgatagccac gagcggtcag ctctggcgcg gttgccgctg gggtgatcat
      961 taaaatgcct tcatcttcat agatgtctga cgctggctgc gttgatgaag aacagaggtg
     1021 accaatcaca tatttaatgc cgtcgttaac gactttgttc gccaccgcaa ccgcctgttt
     1081 cgggtcacag gcatcgtcat atttcacgat ttgcagtttg ttgcctttga taccaccttt
     1141 agcattgata tccgcaaccg cctgctcagc accagtaaac tcctggtcac cgtactgggc
     1201 caccggaccg gacattgcgc ccacgaccgc gactttaata tcttctgcca gagccatatt
     1261 gctgaatgcc agcgcgatac atcctgccag taacgcttta ccctttatat tcatcctgag
     1321 aatccccatt cttctggtta ttacgtgtgt tgtgatgttg ttgttcagca ctttatttcg
     1381 ttttatgcat gactacccgt gctttagcag catactctgc taaaacatac ccgattttta
     1441 tgatattgga atagctattt tgacagttta ttaacaatct gcgtggggat tggcgttttg
     1501 ccggaggggg aaactgatta ttgcagaggc ccaaaaaaca aaaaccccgg attctcatcc
     1561 agggttctct gcttaatagc ggaaattacg cttcaatggc agcacgcaat tttttcatcg
     1621 cgttcttttc cagctggcgc acacgctcag cggaaacacc gtaacggtca gccagttcct
     1681 gcaacgtgga cttgttgtct tcgtccagcc agcgcgcgcg gatgatatcc tggctacgct
     1741 cgtccagacc ttgcatcgcg tcggtcagac ggtttgccgc ctgctcttcc cagttatcat
     1801 cttcaatgcc gtcagcaaag ttagatgatt tatcctgcag atagagcacc ggagccatcg
     1861 gctggctgtc ggaatcgtcg tcggaagaca ggtcaaaggt catgtcctgt gccgccatac
     1921 gcgactccat ctcacgtacg tctttgctgg ttacgcccag ttcacgggcc accatttcaa
     1981 cttcatcctg gttaaaccag cccagacgct gcttggtttt acgcaggttg aagaacagtt
     2041 tgcgctgcgc tttggtggtc gcaactttga cgatacgcca gttacgcaga acgtattcgt
     2101 ggatctctgc tttgatccag tggacggcga aggagaccag gcgcacaccc acttccgggt
     2161 taaaacggcg cactgctttc atcaggccga tgttaccttc ctgaatcaaa tccgcctgtg
     2221 gcaggccata gcccgcataa ttacgagcaa tatgaacaac aaaccgcagg tgagacagga
     2281 tcagcgtttt agctgcttcc agatcgccat ggtaatgcag cttttcagcc agcgcccgct
     2341 cctcgtcagc cgacaacatc ggccacgcgt tagctgcccg gatgtaggaa tccaggttgc
     2401 caactggggc taaagctaaa ctttgcattt tgtcagtcat tcaaatcctc tcaatcgata
     2461 tcttctggcg cttcagtggt agcaacaact gtgccagagc ttaagagcaa cgaggttatc
     2521 attcactgtt ttatcagacc gtgattttat ccacaagttc aatgcaagct tgtgaataaa
     2581 ttacgcacaa aatgtgacat agagatgaaa taccgggaag agacaacggg gtctctttcc
     2641 ctgctacgga acccattgca gggaaagagt ataacacgct tttattattc aggcgtaaag
     2701 tggcgtaaat gttgtaccgt ggcaagccac gctgccaccc agccaatcat cgagcatacc
     2761 agcagcaaca gcaggcattc atcgaacgat aagccattga tatcaaactt cgttccgaaa
     2821 acctgtgcca cttctgcaac cgccgatgac aaacgcagta ccagaatttc tgacaaaatt
     2881 aatgacagca atgcgccaga aaatcccagc aatgcgcctc cgtacaggaa cgggcgcagg
     2941 atgaaaccat ccgtcgcacc aatcagtttt tgtacgttaa tggagtcacg gcgagcaaag
     3001 atgctcagac gcacactgtt gccgatgacg aggaacactg ctgccaccat caacacgccg
     3061 atcatcgcgg aaacgcgccc gaccagcccg gttaacgccg ccagacgggc aaaccagctg
     3121 tcatccatcc gcacttcgtc aatgccgttg atctgggtga tacgatcacg cagcgtattc
     3181 agtgattccg tcccctgaaa atcgagtttc gggatcacca ccgccactgc cggaagcggg
     3241 ttttcttcca gcatatccag cgcaccacca aaaccagacc agttacggaa ctcgcccagc
     3301 gcgtcttcac gagaaagata gttcactttc tccacgccct gctcggcctg tagctgcgcc
     3361 accacgcctg ctgcggcatc atcgtccagc gttttttgga gataaacggt gatttgcggt
     3421 gacggataat actgcgtcgc cgcctggtta acgtttttgt aaaccatata gcagacgctg
     3481 ggcagcgtca gggagatggc gataaccatc accgttaaaa acgtggcgaa cggtttgctt
     3541 ttcagatcct gcaatgcgcc gtggaaggca tagcgcacct gttcgttgaa aacgttggtt
     3601 ttgcgattta ccggttttgg cgaggatttc gcgcgtttcg gcgcgttacg accgccgtcg
     3661 ccagagccac cgaccgattt acggaagcga tccagacgcc cgccaaactg ccgaatatga
     3721 ttgattgcat cgcgcttatt catggcccac gcctccatgc aagtgaccat cgctcagggt
     3781 gagcatgcga taggaacgcc gcgagatcag gttgatgtcg tgcgttgcca tcaatacggt
     3841 taccccaacg cggttaaact cttcaaacag acgtagaatc ccttccgaca acgcgtcgtc
     3901 caggttacca gttggttcgt ccgccagcag taccgcgggc ttgttcacca ccgcgcgggc
     3961 aatgccaaca cgttgttgtt caccgccgga aagctgaata ggaaagttct tcgctttgtc
     4021 cagtagcccg actttatcca gtgccgccga cacccggcga cgaatatcgt caccgctggc
     4081 accggcgata atcagcggga tcgccacgtt atcgtagaca gtacggtcca tcagtagatg
     4141 gtgatcctgg aaaatcatgc caatctggcg gcgcagaaac ggaacttcac ggtttttcag
     4201 acgcgtgatg tcatggccgc taaaccagat tttcccggcg ctgggccgct caatcccaca
     4261 gatcagcttc aggagggtac ttttccctgc gccggaatga ccggtcagaa acgccatctc
     4321 acccggctgc atatggaacg taacgccctg cagcgcctgt ctcccaccga gataagcctt
     4381 gctgacatgt tcaaagcgaa tcattgttaa tcctctcggg caaaaagtgc ctctataaag
     4441 tcgtccgcct taaacggacg caaatcctca atacgttcgc cgacaccaat gtagcggata
     4501 gggataccaa actggtcagc caccgagaaa attaccccgc ctttcgccgt gccgtccagt
     4561 ttcgttagcg tgatgccggt taagccaacg gcttcgtgaa acagtttggc ctggcttacc
     4621 gcgttctgcc cggtgctggc atcaatagtc agcataacct catgcggcgc ttcaacgtcg
     4681 agtttcttca tcacgcggac gattttcttc aactcttcca tcaggtgcga tttgttctgc
     4741 aggcgtccgg cggtatcggc aatcaggacg tcgatattac gcgctttagc ggcctgaatg
     4801 gcgtcgaaga taacagaggc ggaatccgca ccggtatgct gggcaatcac cggaatattg
     4861 ttgcgctgac cccagacctg aagctgttca accgctgccg cacggaaagt atcgcccgcc
     4921 gccagcatca ccgatttacc ctgctgctca aactgacgcg ccagcttacc aatcgtcgtg
     4981 gttttaccca caccgttgac gcctaccatc aggatcacga acggcgtttt accttcaaca
     5041 ttcagcggct catcgacttt cgccagaatc tcgcccatct cttctttcag caggccatag
     5101 agcgcctcgg catcacgaag ctgcttgcgg gatgcgcctt ccgtcagatt ggtgataatt
     5161 ttacgtgtgg tttccacgcc cacatcggcg atcaacagct gctcttccag
//
LOCUS       DAYPHN010000227         5167 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000227 DAYPHN010000000
VERSION     DAYPHN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.227"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1310..1519
                     /locus_tag="ACG0BW_004487"
     CDS             1310..1519
                     /locus_tag="ACG0BW_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261355.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="ACG0BW_004488"
     CDS             complement(1585..2118)
                     /locus_tag="ACG0BW_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261356.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="ACG0BW_004489"
     CDS             complement(2121..3047)
                     /locus_tag="ACG0BW_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFK7261357.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="ACG0BW_004490"
     CDS             3224..3622
                     /locus_tag="ACG0BW_004490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261358.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="ACG0BW_004491"
     CDS             complement(3826..4740)
                     /locus_tag="ACG0BW_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFK7261359.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
     gene            complement(4939..>5167)
                     /locus_tag="ACG0BW_004492"
     CDS             complement(4939..>5167)
                     /locus_tag="ACG0BW_004492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261360.1"
                     /translation="SWRDVKFGNRKPRQSATHQGRKLVVLSRSVFRTHETDRTSLSCI
                     VMQRPENDSDAFFWFGLVFSCLFAFFAPESD"
BASE COUNT         1436 a         1351 c         1104 g         1276 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg taagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DAYPHN010000228         5128 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000228 DAYPHN010000000
VERSION     DAYPHN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.228"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..88
                     /locus_tag="ACG0BW_004493"
                     /pseudo
     CDS             1..88
                     /locus_tag="ACG0BW_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            440..1006
                     /gene="slp"
                     /locus_tag="ACG0BW_004494"
     CDS             440..1006
                     /gene="slp"
                     /locus_tag="ACG0BW_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFK7261361.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1159..1689
                     /gene="dctR"
                     /locus_tag="ACG0BW_004495"
     CDS             1159..1689
                     /gene="dctR"
                     /locus_tag="ACG0BW_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFK7261362.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1750..2778)
                     /gene="chuS"
                     /locus_tag="ACG0BW_004496"
     CDS             complement(1750..2778)
                     /gene="chuS"
                     /locus_tag="ACG0BW_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFK7261363.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MCGDIRDILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRSERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     TPLELKAGDAPVVQMRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAETWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(2827..4809)
                     /gene="chuA"
                     /locus_tag="ACG0BW_004497"
                     /gene_synonym="shuA"
     CDS             complement(2827..4809)
                     /gene="chuA"
                     /locus_tag="ACG0BW_004497"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFK7261364.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGTRLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1411 a         1229 c         1132 g         1356 t
ORIGIN      
        1 acatgggcag aaatgtctga atatcaattc gtagtatttc aatacgagac gcttaaccca
       61 aaaaaaatgt catacgcacc ttatttaaag agatctctac ctgatgaaaa aactataaat
      121 tatggggtgg aaatagaaac accaactggc aaaagaattc gcttatcaaa tcattaccag
      181 aatataatcc cataatatga acattagcac tttataatca ttgaataaaa atagatttta
      241 tgtaccttta aaaaaaggct attttatggg gcgatggata ctcatgtcac gccccaaaat
      301 taacagaatt aaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
      361 gcatatcgcc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
      421 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
      481 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
      541 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
      601 ctttggcggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
      661 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
      721 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
      781 cctcggtacc attcagggtg aacaacctgg ctttatcaat aaagtcccgt ataacttcct
      841 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
      901 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gcgcgcctta
      961 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgagagat
     1021 gcagatgctt tctcagcatc tgcatcatgc attacatcaa attaatacac agccagctaa
     1081 ctattattat aagccctgtc ctgttaatta cctttggcaa actgattata aagttaatgt
     1141 ccgcaccagg agtcggttat gtttcttata attaccaggg atacgatgtt cttcactgcg
     1201 atgaaaaaca ttctgagtaa aggtaatgtc gttcatatac agaacgaaga agagatcgac
     1261 gtaatgttgc atcagcatgc cttcgtcatt attgatacat taatgaataa tgtatttcat
     1321 tctaatttgc tcactcaaat tgaacgatta aaacctgtcc atgtgattat tttctctccc
     1381 tttaatatta aacgctgcct ggggaaagta ccggtgacct ttgttccgcg aactatcacc
     1441 attatcgact ttgtcgcact catcaatggc agttactgct ctgtgcctga agcggatgtg
     1501 tccctttcgc gcaagcagca tcaggttctg agctgcattg cgaatcaaat gacaacggaa
     1561 gatattctgg agaaactgaa aatatcgcta aaaacgttct actgccataa acacaatatc
     1621 atgatgatcc tcaatcttaa gcggatcaat gagttggtac gccatcagca tattgattat
     1681 ctggtgtgat cgattgatta atataaagag gcccagcaat aaatagccag acctcccgtt
     1741 aattatacgt tatgcagtaa cgacttccgg tatcagcgaa gcaatttgct tacgccattg
     1801 cgcttgctcc tgttcgcctt ctgtacgttg accataaagt tgcgctatct gcgtaccatc
     1861 atgggcaaac aattccagac tggtcacata accgtcacta gcaggcttac gggttaccca
     1921 ggtttcagca atgctctctt ctaatagatg aagagtaaac gtcgggttaa aaatattcag
     1981 ccaacctttc attggcacca ctttttctac cacgccagtg aaaatctgta cgcagccacg
     2041 gttgccaaca aacaccatga tttcattgcc atcctgctgt gcagattcaa gaatttgcgc
     2101 caacgcactg ttggatactt tgcaggccaa atcgtctgcc accagattga acgcctgttg
     2161 gcgcgtcagg ttgtggcgct tgagcaacgt aaaaaactga tgaacgtcgg tcatcgcccg
     2221 ccactcttgc tcaaccacac tggcatcggc tcgcatttga acgactggcg catcaccggc
     2281 ttttaactca agcggcgtat tctcatcggc gataaaccgt gccagaagct cactccatgc
     2341 cgccatatcg gtattgtcgg tggcataaac ttttagtaat gcatcgccct gatgatcaaa
     2401 gaactgaata ctctggcgtt cgctacgagt cgtattttct ttaatgtgaa aaacactggc
     2461 ccattgattg agaaacagac gcaaatccag cgcgcgcgga ttgaggatca atccggcatg
     2521 tccgttcaaa tgctggtttg tgaacgcacc aacttgctca tggactgcat attcattacg
     2581 acaaatacat ttggtctcgc caacactttc gagcgccgcc agaatgtcac ggatatcgcc
     2641 gcacatccgc cacgcatcgt gcgtgacgcg tgcaaatgcc agttctgctt cgctaatatt
     2701 cattaaacct gcgatgtcac gcgcgtactt tccgggattt tgttctttta actcaagcca
     2761 gcgtgtgtag tggttcattg tctctcttcc ttccagataa atgccccgaa atatcggggc
     2821 agatgattac cattgataac tcacgaaaat ttttccatta cgaccatcct gtgggatgcc
     2881 ttgtggcgac cagtactctt tatcgaaggc gttgcccaat accagagtag tggtcatgcc
     2941 tttgagcgcc tgctgccctt gataactgac gtagaaatca ttcacaccat agccaggttg
     3001 tttgctgtaa ctgctgctga tatgtgttga gcgatcggca aacgtaccaa cccacccaac
     3061 agagaagcca ctgtgagcga tcggaatatt cagggtgctg gtaacggtat ccgggttaat
     3121 actggagata tattcgccgg tatcggtgtc tttgccgcgg gtacggttat aggccacatc
     3181 aaggctaaac agatcagtgg tatatttcgt catcacatcc cagccccaga ttttggcgtt
     3241 cgggacgtta tacgacatag ttgtcgccgc cgcgaaatcg acggtcgtgg agatgtaatc
     3301 cttcgctttg gtatcaaagt agctggcttt aaattccaga gcatcattgg acaacatcag
     3361 gtcatcaaaa cgcagcccaa aaccgtactc ctgagtttcg ttagtttccg gacgtaagtt
     3421 cgggtttggc acccaatagt tggtatagaa gcgaccaatc gagaagtgct tagaatcgtt
     3481 atacatttcg cccatcgtcg gggcgcggaa tgcctgggca tatgagccaa ataacatcag
     3541 ccagttagtc gggttgatag tcatccccgc gcgagatgac catttgtcgg catcaacatc
     3601 tttgtagccg tcgctgctac cgcgataact gtcatagcgg gttccgccaa gcagggtaat
     3661 cggcagatcg cgtaaggtga tctcatcctg tagccagccg gagctaaaat cgatttttgc
     3721 ttgcgggaag cccgtcgtcg cgccgcccgg atgttgttcc tgacgataat actcaccgcc
     3781 atatgtcagt aagtgagaag cgaaactgtc ggcaaataga gtggaacggt tctccagcct
     3841 ggttcctttt gttatctgtt cacgatactc gccggaactc cccgtgtttt gcgcattaat
     3901 acggacttcc gaccagtaaa tttttgcatc tgcatttaac cagtcgttgc cctgcggggc
     3961 gagtttataa gaaagctgcg catcgcgttg aattgttgaa cgatcaacca tcgggttgct
     4021 gctatcagaa gcctcaacag tctgcggatt ttttggttca cgcgcgtcgt tgttgtagta
     4081 acgcactaaa ccgctcagag actgggctga atcaatttgc caggtccctt tcgccagcat
     4141 gttattaatg gactcgtcat tcggcgcggt ttcaccattg ctctggcgta aatcaccccg
     4201 atcgcgactg gaccaggcca caataccatc cagattttca gttcgcccaa acgcgctcgc
     4261 gcctaatccc aggctatggt cccccgtgcc gccagtacca aagacacgaa aaccactact
     4321 ttgtccttcc tgcaataaat cttttgcatc gaccgtatcg taggagatca ccccacccag
     4381 cgcgccactg ccatacagta atgctgaagg tccgcgaaca atctcaacac gcttgatcag
     4441 cgccggatca agaaaagtac cattcaggtg tccggtatcc gttccctggc gaacaccatc
     4501 gacaagaacc agcacgccgc gatgatcata gccacgcata tttacatcct gaccgttggt
     4561 tcgtccggta ccatcaagag taattccagg aacatgacgc agcagatcgg tggctgaagt
     4621 agccgtttga ttttcaggag cggaagtgtc gatgacgctg accatcatag gcgcttcgaa
     4681 ggaactacgg gcattccccg ttgccgtaac ggtcatggtt tcagtagcaa aagcaaacgt
     4741 tggcaaggtg gcagaaacag ccaaagccaa caaactcaaa cgcaacgagg taaattgcgg
     4801 acgtgacata gcgattctcc atgaggaaaa taaaaaatgc tggctgcctg ggttaatacc
     4861 ggggtggctg gcgaaaaata aatttattta aaattcagag cgataatatt cgccgggtga
     4921 gtttacaggc gtttatatcc catgaaattg cctggtatct cggcaaatac gcaaaagcac
     4981 gccctctttg tcaggcaaaa cgatatgccc catcaggagt gaagagcgct gtgcgttacc
     5041 ccttgtaaat gatatggctt tgttatccat atggccgtat tttgttaaat gcaaacgtga
     5101 taatgagaat tattatcgat caattttt
//
LOCUS       DAYPHN010000229         5124 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000229 DAYPHN010000000
VERSION     DAYPHN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.229"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..343
                     /locus_tag="ACG0BW_004498"
     CDS             <1..343
                     /locus_tag="ACG0BW_004498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261365.1"
                     /translation="RKQLNNRSTKRQRFHPIWSKKLTFSHITSYTRNSTQQTAPQRPI
                     FTTDDPTHYQQKHTLKIAPVALHNHINNLFSGITKQLNNTHAISKNNPPLLPRLPKRP
                     TSSPQATGQPT"
     gene            1420..1749
                     /locus_tag="ACG0BW_004499"
     CDS             1420..1749
                     /locus_tag="ACG0BW_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261366.1"
                     /translation="MFTNIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMISDLGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFE"
     gene            1903..2214
                     /locus_tag="ACG0BW_004500"
     CDS             1903..2214
                     /locus_tag="ACG0BW_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261367.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWVAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            2341..2736
                     /locus_tag="ACG0BW_004501"
     CDS             2341..2736
                     /locus_tag="ACG0BW_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261368.1"
                     /translation="MTAKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQAST
                     IVRKGDASGGEYATQYQSIFDATDPLLLKIENYFRQVDEEVFETMMNMVNTVFASSLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            2854..3228
                     /locus_tag="ACG0BW_004502"
     CDS             2854..3228
                     /locus_tag="ACG0BW_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261369.1"
                     /translation="MGLDIYIETQPKNDLNNEASRKQVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICFLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVDKLKK
                     LVEDLIRNHDFHNNRLTFCAWW"
     gene            3564..3845
                     /locus_tag="ACG0BW_004503"
     CDS             3564..3845
                     /locus_tag="ACG0BW_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFK7261370.1"
                     /translation="MTDFTITPKAQNVFLESWLDLPETEQQEMDHVDYDEQVSTRFFH
                     FEGCVYDIADFMRDDRFPEWHASYPLNAFAMLMIRVDDSGDTIDIGLLH"
     gene            4050..4532
                     /locus_tag="ACG0BW_004504"
     CDS             4050..4532
                     /locus_tag="ACG0BW_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261371.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRHNETVELSEQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYAAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
BASE COUNT         1562 a         1201 c         1123 g         1238 t
ORIGIN      
        1 aagaaaacaa ctaaataaca gatcaacgaa acgtcaacgc tttcacccga tttggtctaa
       61 aaaattgacg ttttcacaca tcacttctta tacccgtaac tctacgcagc agacagcgcc
      121 acagcgtcca attttcacca cagacgaccc aacacactac caacaaaaac acacgctcaa
      181 aatcgctccc gttgcgttac acaaccatat aaacaactta ttttcaggca taacaaaaca
      241 actaaataac acacatgcca tttccaaaaa caacccacct cttctcccca ggctaccgaa
      301 aagacccacc tcttctcccc aggctaccgg acaaccaacc taactttcac aggcaatccg
      361 aaaacattca tacgcgcgat acccacgaca cgccattccc caaacagcga agaaacacca
      421 gaatcactga aaccccaacg aagcctgtaa ccgcaaggat tctctcaatc ccaaaagaca
      481 cgaaacataa caatcacggg aaatgccatt acccaataca cgaataacta caaaagcctt
      541 tcacacactc cagacaaaca actcaaacac aacaacgaaa ggaatcgccc acctgccatt
      601 actccataca cggagaagaa caatcaccaa aacgacgaga acaccccata gaagaaccac
      661 cgcaaaacaa acgaaaccac atacaccgac agagaaaaca acacacccaa ttcaccaaca
      721 gaaatagtcg ccgtatagaa cattctggaa ggagtgagca ttaacgccta tagagaggtt
      781 gagacagata aaacacaaca tgtatagcga agaagccagg tatgtataag tggggaggga
      841 aggaggggtg tcgcctcctt tttcgtattt attcaactcc tgattttatc ccccccgtaa
      901 cacccctacg gtcagccttc ggtccataca gggaaaaggt tgcatcccgc tacagaaacg
      961 gctgggttgc cttcggggaa cggctggagg ttttcaggga aacggtcgag ttgcctgtgt
     1021 ggatttcggg aaaaggctcg atgccggttc aggaacggtg gcgtaccggt atagcgagag
     1081 ggagagttgc ggctacccac ctgtaatgtg cgggaagaag agaggcgctc acgtaggcgg
     1141 acttgcttac cgctggattc cctctgaatg cccagctaca gcgtaattgt gccagacagc
     1201 caacgggtca caatcgattt cagtaggttt ttggggagag gattactctg ccaccagcca
     1261 ctttcggcca ctaaatcaga ataatgtaaa aagtgcactc ggtactgaac ctgaaaactc
     1321 actgccaaaa ccacattcac cggaataaaa tcctttttca cctgtgcgtt attgcgataa
     1381 taacaccatc aagaaaacat gttgtttaag gattgcttta tgtttacaaa tatcgacatc
     1441 aaccaaatca agaaattaac tcaaaaagag tttgatcagt tttatgagtt agaaggttgg
     1501 tcttccactc tgatcaattc aagatgggtg ctggaactga tgactcgtga tgacgcacct
     1561 gctttgatga tcagcgacct gggtgaagat gctgacttta tggatatgag cgaattttgt
     1621 gtggacacat acaaccgcag ccagaagtac tacttcacat gcgatagcga aaatgacgtg
     1681 atttctaagg tctatcttca cctcgtccag cattgggacg ttcaggagtt tcttgaagta
     1741 ttcgagtaac cccaaccaaa gccagcattg ctggcttaat ttctctatag ccgcagggaa
     1801 gaacctgcat atagctcatg cacctgatca atatgctttg tagccgtttt cttactggta
     1861 ttatttacgc cattgagaaa acaagttgtt tacggagttg tgatgaaaaa atcattggtt
     1921 cttggtctgg acaaagacca gaagagaaaa gagaagcctg cgctggttgc tcaattaact
     1981 ttgctggaca tcgttgccaa tgggacctct attcgtctgt tccgtgaaac agcggtgtct
     2041 ttcgataaaa acacctttac tcgttatgta atgaatgttc gtcgccagcg tggaaaaggc
     2101 tgggtggcat ttcaaagaat gtggccggaa catcagctcg aacttgcttt gatggaagtt
     2161 aaccgcgtgg cccagcaaga gattcagaga gcatcagtga tggcaatagc ctgataatgt
     2221 gcaagtggta attagtcgac cgtacgacag ccccgccatc cttacggggc ttttttgtat
     2281 tgtaagtaca tacttacgat catagaatag aaaaataacc aatacactac ggagagtgaa
     2341 atgaccgcta aacgcgaaaa actgacagtt gatgtttact atgcctctga aaccgccgaa
     2401 ggtaagaatg tggcaaaaat caccgttgtt acgtacaaca ccgaaactgg tgccgaagtc
     2461 caggccagta cgatcgtgcg taaaggtgat gcctccggcg gcgagtacgc gactcaatac
     2521 cagtctattt tcgatgcaac tgacccgctg ctgctgaaaa tcgagaacta cttccgccag
     2581 gttgatgaag aggtgtttga aaccatgatg aatatggtta acaccgtatt cgcctccagc
     2641 ctgaatacca acactacctg gattggtcag tacggcctgc gcattacctc cggcattcct
     2701 gctgacacct taatccctga aagcgtattc gcttaatcct ctttgaatgg cgcgtaaacc
     2761 gcgccatttt cttatgcccg ataacaattt gttttctgcc ttatctgatt tgtgaaaatg
     2821 atttcactga agcaacttaa taaggaaacc atcatgggac ttgatatcta tatcgagaca
     2881 cagcctaaaa acgatctgaa taacgaggca tccagaaagc aggttgctta cttccgtaag
     2941 ttcaatgcgc tcgttgggtg gatggagcgc aacgtaggtg aagtcgaaaa ttgtgagctt
     3001 ttagaattaa cgatgaatga catttgtttt ctgaaggctc atttgatgca cataaacgaa
     3061 agcaattgcg aagagtactt gcctactcgg gaaggttttt tcttcggcag tcaggagtac
     3121 gacgaaggtt actggcatga cgtggataag ctgaaaaaac ttgtggaaga cctgatcagg
     3181 aaccacgact ttcacaataa cagactgacc ttctgcgcct ggtggtaaat atgggcgatt
     3241 tcaagaaacg cctgaaggaa agagccgaga tggtcagaaa gcaaaacgcc tcttctgtca
     3301 tcagatacgc aaggcagttt agtcgcaaca acaaatcagt tgaggaaaag atccttagcg
     3361 taatcgggcg ataattatta aggccaccag cattggtggt cttaaatgac ttttaataac
     3421 cgaaggttag tttgaaacct ggctatcaac ttccagaacc tttcttgtgt attcagcctg
     3481 tattaacttc ttttatgcct tatctacact gcgataatta caccaacaag aaaacaacat
     3541 gtttacgcag tgaggatatg ctcatgactg atttcaccat tacccctaaa gcacaaaatg
     3601 tattccttga atcatggctg gacttgccgg aaacagaaca gcaagaaatg gatcatgtgg
     3661 attatgacga gcaggtaagc acgcgattct tccactttga gggctgcgtt tacgacattg
     3721 ccgacttcat gcgagatgac cgcttccctg aatggcacgc aagctatcca ctaaacgcct
     3781 ttgccatgct gatgatccgt gtggatgatt caggggatac catcgacatc ggtctgctcc
     3841 actaaaggct aaggccacca atgctggtgg ccttaaatga ccatcctgtt tccctcaggc
     3901 taataacacc aacctcttac ctccaggcaa ccgacaaacc cacctgttcc cgtccggcta
     3961 ccgtaacttt ccactttgac gccttattcg tacaacgata attaacgcca acaagaaaac
     4021 aatttgttat ttacgataag gaattaatca tgaattttat cgctactgta aacgcacccg
     4081 cacatggcaa tatcgctgta acgttctctg acattgaaaa acgagtactt ggtgcctggc
     4141 gtcacaatga gacggtagaa ctgtcagaac aagaaaaatg cattattgca cgcgacatca
     4201 ttggcaatcg tcgttactcg cgggtatttg agaaagcgta tgtggtaaat tctggattcg
     4261 gaacgttcgt ctttccggtg cgctccgggc gattctgcca gtccaagctg attgagttcg
     4321 ctacgcagat ttctgtctgg attaaaactc aatcgtcgtt caaattttcc gacgatgaag
     4381 cagtatcaca ggggatgcgg atcgccaaca atgcaataaa atgcaaaaac attacgtatg
     4441 ccgctggcgt tgatacatgg aaactgtttt gcgctaactt tatgctgaat gtatacgcaa
     4501 gcaaccgcat ccacatcctt gatggcgtgt aactgagaag agggccagaa acggcccttt
     4561 ctctatagcc accagctgcc gcagggaaat tttcagaaac ggcgaggaac gtattcatga
     4621 gccgacgggg aacggccagg attttttcgg gaaacggctg cattcgcctt tgtgtagaaa
     4681 aaacatcggg aagctggtgg aatccaacca gcggttgtcg gacaggtgag cggggaaaat
     4741 tatgatgact ttcgtcgcct gagacatcca gatttctttc gtagcataat cacatacgtg
     4801 attaagtggt gtgattatgt gaaaaatcac gcgcacataa tacgcgagcg gatacggaac
     4861 aaaacaaaat gccgatccgc gccgacaaat aaacgcggat cataaagcaa gactaaaagc
     4921 caatggttaa ccatactcta tagcgcaata taacgcgttt taagcgcgtt aatgtgttaa
     4981 gtaatgggta tgtactggta aggatataaa agcgcgtcta tggcgttatt ttggcgctta
     5041 tttttatgtt gttggagtga gttaaagaca ataaaaaacg cgccatcatt ggcgcgtttt
     5101 ggtgggagta ttggaaacga aaaa
//
LOCUS       DAYPHN010000230         5096 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000230 DAYPHN010000000
VERSION     DAYPHN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.230"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(78..1148)
                     /locus_tag="ACG0BW_004505"
     CDS             complement(78..1148)
                     /locus_tag="ACG0BW_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261372.1"
                     /translation="MNKYIIQWCLTVFIFSFSGATFAAPKGICTSDNGAFHSTLDFSG
                     YLITADENRVGETFNKTVTNGDSYPAHCHCDTGKVGEFPYIYYTARINEALSYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIVPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVAGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSNVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            complement(1159..3681)
                     /gene="lpfC-O113"
                     /locus_tag="ACG0BW_004506"
     CDS             complement(1159..3681)
                     /gene="lpfC-O113"
                     /locus_tag="ACG0BW_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFK7261373.1"
                     /translation="MMTTRIKVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVIQAQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKDLGALGAISFDVTHARAKFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNNASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFKVVNAKGKPAPFGAIAAVQNTSSADSGIVGDLGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(3706..4437)
                     /gene="lpfB-O113"
                     /locus_tag="ACG0BW_004507"
     CDS             complement(3706..4437)
                     /gene="lpfB-O113"
                     /locus_tag="ACG0BW_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFK7261374.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIVENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMIWRFINDYGAVSDPIKMTL"
     gene            complement(4485..5057)
                     /gene="lpfA-O113"
                     /locus_tag="ACG0BW_004508"
     CDS             complement(4485..5057)
                     /gene="lpfA-O113"
                     /locus_tag="ACG0BW_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFK7261375.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         1372 a         1205 c         1111 g         1408 t
ORIGIN      
        1 ttgtcatgca aagtgaaaac tcgcggacaa aaatgcgtaa gatgacaacg gtgtgaggaa
       61 caccgcggtc agttcgttta gcgtacaatc tcaattgtta tcgttgcagt cgcggtaaat
      121 gttcctggcg cggggcgagc ccccgtgagg cttgcaggag cagcagaaaa agtgacgcta
      181 ccggcttttt caccaaagac gtttgattta cccatatctg tcggtagttc accaccgtta
      241 acgttgattt ccttattatt tttatcataa atttttatcc caacgttcgg gtttgatgtc
      301 acgaccattg agttgtcagt actgggttcg gtagcataaa aagaggtact gatatcatca
      361 gtatttagta ttcctaaatt ttcacactca atggtgacgg ttttggtaat tttatgcgtt
      421 gtcacggtac ttccagctac cgaagaaaaa tctgctgcag gaatttcttt aaaatcaaaa
      481 ttaatgggtc ttaaactgtt aatttcacag ctttgcggtg cagtgatatc gccacgaata
      541 taaacatcgg ccataggttg tgagtaatca accggagtgt cacggcttat agttccgtac
      601 aattttgcca cgagagtttc agggacaatt aactttccag caaaagtctt cttaatataa
      661 aaataaatta ttgcttttgc accactggaa atacttaatg ggtccgtgcg actacattta
      721 tatgaacctc cagtaggcct gttagcatgg tattcaaaag gtgcattaat gtatcctaca
      781 ccgagaaggt ctatcgatat tccaacatcc agattaggat tcaaattaaa gtagtttaca
      841 ttagaacgaa cacccgcata acttaaggcc tcgtttattc tcgctgtata gtaaatataa
      901 ggaaactccc ccactttccc tgtatcacaa tggcagtgag caggataaga gtccccattt
      961 gtcacggttt tattaaaggt ctctcccact ctgttctcgt ctgccgtaat cagatagccg
     1021 gaaaaatcaa gtgtgctatg aaaggcccca ttatctgagg tgcagatacc ttttggggcc
     1081 gcaaatgttg caccactaaa ggagaagata aaaacagtca agcaccactg tataatatac
     1141 ttgttcataa aaatcctttt aatgacatgt cacaccttgt tcaattaagc cgctttcact
     1201 ttctggtatt gaaagtgaat aatcgaaggt gcatgtagtg gcggcatttt ccccccagga
     1261 gagcatcact tgcccctttt caggaaggcc ggagagataa agctccccca ggtcaccgac
     1321 aatccctgaa tcagcagagc ttgtattttg tacggcagca atagcgccaa aaggtgctgg
     1381 cttacctttt gcattcacca ctttgaagag tacgcgatag cccacgtggg taacaaattc
     1441 tgctttaacc accgcgccac gggtgggaac catcgtaacg acggtattat caagttctat
     1501 attcgacttc gagaaatagt tatcgctgat tgctacccgg ttttcatcat aagggtttag
     1561 ctgtgtcttg accgtataac cacgccagtc cgtcgcagca ccacgcatat tgtctatttc
     1621 taacccggca gcccctggtg ctttaacaag agctatggtt tcacccaact cctggcttaa
     1681 cgttacgcct tcgctgtgag ccagaattcc ccctctgata ccataattca gcctctgaga
     1741 attttggctg tagttatagc tgccgttcac cgtgccataa gtgccatcat aggtcaggga
     1801 catattaccg ccaacctcat catcatggct gttataggac tgcattaaat tccaggaaag
     1861 gttatttctt tccagcgccg taccgctgag tccaagttga tgtgagctac cggaattatc
     1921 gttttgcgat acagaatagg tggcgtaact attcccccac cagcccgata aaggaacaga
     1981 taaagagagc atgtagatct gctcactggt gttatcactg gcattgttgt tccccgtccc
     2041 ctgccgggca gtattggaat aactccagga gacgttccac gagagacgct tccagctact
     2101 gctgtagcca aattgcatta atcgttcaac atcatcggta tgccagtact gctggcgact
     2161 taatgtcaga tataagttgc cataatctct gcccagtgat tgcgttaacg ttccttccac
     2221 acggctgcgg cggttaccgg tttcccagaa atcttcagga ttattacgcc gggacgccca
     2281 ttcattgagg gtataataac cttcggtgga gtaacgatag ccaaccaggc gcaagctggt
     2341 atccgtatcg tcaaagcgtt tcgagtacag gaagcgataa gactgaccgg tttccgtatc
     2401 atcatggcta aatttcgcgc gagcatgtgt cacatcgaaa gaaattgcgc ccaatgcgcc
     2461 aagatcttta ccgacaccca aagcggcaga accatacttt tcagcagcct gcatacctcc
     2521 atataaggtg aaaccgtagg gaaaaccatg aaggatctga gcctgaataa cgtcaggggt
     2581 aaagccatct tcatcgcgta attctcccac actgacatca acatcagttt gaccttcacg
     2641 tttgaggatg gctaacgacg cataaggttg agtaaatcgt tgttcgctac cgtcttcttc
     2701 tttgatggtt acgtccagat caccacctgt actggcggtg ttgagatcgg taatttcaaa
     2761 agcaccttgt ggaacggctg actgataaat cacgtagcca ttctggcgaa taatgacagt
     2821 ggcgttagac tttgcaatcc cacgcactac gggggcaaat ccgttttggc tggcaggcaa
     2881 catatcgtta tcggtataca agcgtgcgcc acgaatttgc gtactgtcga agatatcgct
     2941 cgccgtccac gtatcaccta tcataatctg gcttcttaac gcagctatat cacgttgtat
     3001 ccaggtgctt atagagttgt attctgcact gccatcggtc gtatttaagg tgctgtaatt
     3061 gcgtaatcgc cacggcccaa tattcagtcc attgcgcaga ttgagataac tggtatctgt
     3121 ctcttgtgaa ccataatccg aacgtagagt atgtgaacca gaaaaatcgt agttgatggt
     3181 gagcgcgtta atcccctcat cccaacgcga ggggctgatg taatcataag ccatcgcatc
     3241 catatagatt tggggaatgg agatattcag ttgcagttta tcgctgtcga actgccatga
     3301 tgagccttca atgcgtggcc caggatcaat acagactgtt tcatctacat cattgagcga
     3361 tttcagcgca tcaactttta cgccaaaggt ggttagcgtt tttttaatag cgacaaagca
     3421 ggcgtgtaaa tcagcatcgg gattatcctg aacaaaggtc acatcctgac gttcaaacat
     3481 tttaccgttg ataaaaatat caacgctata aacacctggg ggcatcaggt ttgcccgatt
     3541 aaactggcga atatcgactc cggattgatg gtcgatttcc agaagcgccg gattgaatac
     3601 actgacctcg gccatcaggc tttgcgagaa aatcagacac gtccctgccg tgaggccaac
     3661 ctttattctg gtcgtcatca taatacgcct atccttttcc cttgcttaca gcgtcatttt
     3721 tatcgggtca ctgactgcgc cgtaatcatt aataaatcgc cagatcattt ggcctcccgc
     3781 agaattagca ggtaaaggta acgtgagcgt ttcaaaagga cggatcatgt tgaggggcac
     3841 atcatgacca tcaacgatga catccatcac actgatatga atcgggttgg gattctctac
     3901 aatcaggttt cgcccttccc ggtgccactt caccttcggt gcagcatctt cgggaatcag
     3961 gcgaatgctt gccggtcgcc agaacagctt gatacgcgtt ttaactgcga tttccagtga
     4021 gttcgccgct ttgttgctcg cagagggcgg aatattttta atattcagcc agaagactga
     4081 ctcgcgatca tcaggcaatg acacaccttt ggtatgaata atgcgtaata ctttttcttg
     4141 cgcgggttct aatcgtgata ccggaggaat aacggtaaac ggtgctttgc ttttatcctc
     4201 tttattaaac ggatctaccc atgcctggat gagataagga attttttctt ccttgctaaa
     4261 tacagaaata gatttatctg ggttattact taagtaaact acccgtgtac caccaatgac
     4321 aacgcctgca taggttaatg ctggttggac taaaaaaaca ctgagtataa tcgcggtaaa
     4381 taacttacat gaaacggttg ttttaaagag cctgattaaa caactttttc tggacataac
     4441 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
     4501 acagtaaatt gtgaatcggc gttcgccgta cccgccgtta cagttgcatt ggttgccaca
     4561 tagcgtgcaa taaacttcat ttcagccttg ccttcagcgt cgatagcggt ataggcagaa
     4621 tcgttataaa gtttaacctg cgagttatct gactggttaa aaatacgaat accgacaccc
     4681 gttgccgcaa tcgcttgtcc tgtaccggta aaatcgccag ggttgcccgc tgtaagcggc
     4741 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
     4801 gcctgagtgt aggttgccgg gcacttttca agtttaatac tgaactgctg tggtgacgcc
     4861 agactaccta cacctgaaaa agcattttta ctgactttac ccagtggtac ggtttgatcg
     4921 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
     4981 ccatcttctg ccagtgcatg tccggccagc attaaagatg ccagagtgaa tgcaccgcct
     5041 ataatattac gcttcataaa aactccattg atataatata tcctttacca tgaatc
//
LOCUS       DAYPHN010000231         4832 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000231 DAYPHN010000000
VERSION     DAYPHN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.231"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            488..1027
                     /locus_tag="ACG0BW_004509"
     CDS             488..1027
                     /locus_tag="ACG0BW_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261376.1"
                     /translation="MSDYSYLSILKTCAKKLARIQKIKLSKALEIVARDAKFTSYHEL
                     SEVAKRAPLEQRLVFAAIGEDTQEDVIYRDEIFYAIDREVEDMLSGDIAETNAYGFSI
                     ENLEATSINYDPKRGILVVQVIFDYQGEQDSERPFSGSSFEIVAKMELLLRDNVWSLV
                     ENSLELTSVNSNVDNDWYD"
     gene            1236..1628
                     /locus_tag="ACG0BW_004510"
     CDS             1236..1628
                     /locus_tag="ACG0BW_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFK7261377.1"
                     /translation="MLKQLVEKCYVQKPLDMFSMTAGKLTGLDQSGPKLASIICRGIE
                     QAKDVQLGELLFACGIYGVEEEEAWLLAKRFSNLEALYGASIDSLMSYNLLNEAVAVN
                     TYNFFRHPLNVSALNELQTEGGLKVRHG"
     gene            1963..2178
                     /locus_tag="ACG0BW_004511"
     CDS             1963..2178
                     /locus_tag="ACG0BW_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HFK7261378.1"
                     /translation="MKTSADSNDAFPESGNVRMRQVVQFLAMSESSVYRLIKNTDFPR
                     PVHLSSRLVVFDAAEIRQWQQRRAAIR"
     gene            2214..4283
                     /locus_tag="ACG0BW_004512"
     CDS             2214..4283
                     /locus_tag="ACG0BW_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFK7261379.1"
                     /translation="MKRAPFLCKQSPDRTLEVVILAGSLAWETSHVWRKDPDREDDVP
                     PMVLGPDELADLSNLTIIRPDTLYVRVLRTGDISEEELLKIAVKLAHAGVQMARLMTP
                     DGELLENWTGQLERLRQERPSDILPDHFRLDEEALWFDKLTERRDGESDVQPQRICSP
                     LRVTAITCDSHDGSYGRLLEWHTTTGQLRRWAMPMAMLSGNGEELRRILLENGLTYIS
                     TRPALRSLLCEYISRSLPGRRVTCAEKTGWHNGVYVLPDEVIGPDGDNVILQGSHYLT
                     GGFAQSGTLAEWQEQVAALCAGNSRLVFAVCCALAAPLLRLTGTGGGGFHLRGESTDG
                     KTTVMKVAASVCGGTDYWHSWRATGNALEGIASRHNDALLPLDELREVDPREAGMIAY
                     MLANGQGKGRARTDGEVRNRRHWTLLLFSTGELSLAEHTECAGERLYAGMDVRMVQIP
                     SDTGQHGSFEQLHGFASGQQFADTLCDRVARFHGTAFRAWLAFLTSDLDASTTLAREL
                     LRRYQTALMPDNAGNQVQRIVARFALLAVAGEIATLNGITGWQEGSAYGAVQICLHAW
                     LNERGHIANQEDEGVLAQIKRFITAHQYSRFASWDGPDRPLNMAGFRRVEKDPLTGEE
                     HTLFFILPEGWREICRGFSPARAARLCQEAECLLPGSDGKYQSQVRLPEIGKARVYRL
                     TSRILSI"
BASE COUNT         1204 a         1260 c         1255 g         1113 t
ORIGIN      
        1 tttatttcta gctatttttg ttgagaagag atccattatt tacctctata aacactttgt
       61 aaaactaaac atgtgtattt tactgcgctg attgtcgaac atatgcttca aattgtaatg
      121 cggtattcta catcagaaaa acctctttta tattttcctc attaaattta tttacaccga
      181 aaaatagccg catgaatcat gtctgaacag atgaacttct ctaatatagc ctaaatacct
      241 acgactgcgc tataaagagg gaacctcaag cactaatagc acaaagaaaa tatgcgaaca
      301 caaatttgac gcgaaactat gacttagata ggatgaatct aggtggtgat ggtcacctgg
      361 cgcctgtctc gcaaagcaat agcccatcgt tcagaagtat accaaatact tggccgccat
      421 ccattatgac gctgcccggt tagcaggcta tcggtatcca aagcgttact ttattggagg
      481 tacagccatg tctgactatt catacctaag cattcttaaa acgtgcgcta aaaagctggc
      541 tcgcattcag aaaattaagc tttccaaagc actcgaaatc gttgcccgtg acgcgaaatt
      601 caccagctac catgagctgt ccgaagtcgc aaaacgtgcc ccgctagaac aacggctggt
      661 tttcgctgca ataggcgaag atactcaaga ggatgtgatt tatcgcgatg agatttttta
      721 tgccatagac cgcgaggtgg aagatatgct ctccggcgac atagcggaaa caaacgccta
      781 tggttttagc atagagaatc tggaggcaac aagcataaat tacgacccaa agcgaggcat
      841 tctggtcgtc caagtcatat ttgattatca gggtgaacaa gattctgaac gccctttctc
      901 tggttcgtcc tttgaaattg ttgcgaaaat ggagctactt cttcgcgaca atgtatggag
      961 cctcgtagaa aatagtctgg aattgacctc tgttaattca aatgtcgata atgactggta
     1021 cgattaagac atataacagt aggaggcatt ccaaattttt ggcggagtgc ctttttagta
     1081 actatatcca gatgaaatca tcgagttatc acatgagcat ccatcttacg tgtactcaaa
     1141 acacttatgg gcttctaaat tcaggaagtc cctactccgc ttgctggcgc ttctttgagc
     1201 ctcgtggcgt ccatatacac caattaggcc agaccatgct caaacaactt gtcgagaaat
     1261 gctatgtgca aaaaccactg gacatgttct cgatgacagc aggcaaacta actgggcttg
     1321 atcaatctgg gcctaaactg gcttccatca tatgcagagg gatagagcag gccaaagatg
     1381 tacaactcgg agagttactt tttgcctgcg gtatctatgg tgtggaagaa gaagaagcct
     1441 ggttactagc taaaagattc agtaatcttg aggccttgta tggtgccagc atagattctc
     1501 tgatgagtta taaccttctt aatgaggcag tagctgtcaa tacgtataac ttcttccgtc
     1561 atccgctcaa tgtgtcggct cttaacgagt tacaaacaga aggtggattg aaggtgcgcc
     1621 atggatagaa ttaacaccaa ggctgtgccc ggccttttat ctatgcaacg tagccgatga
     1681 cagttaacgg tgagacagca acacctgttg taacatgttt gtactcgtgc attgccatcc
     1741 gtaggcctaa acatatctga ctcccacagg atggggataa aatacaacat ttgcattttc
     1801 aatttgttat cgaaaaatca ttagtcctga tgggaaataa tatcttcccg gactaatgat
     1861 tttctatccg gactccactt ttttatccgg gcctatacct cccctcatcg ttccgcgcta
     1921 taaccgctcc gacactctgt tcccgtcccg tgaggtgcaa tcatgaaaac cagtgcagac
     1981 agtaacgatg cttttcccga aagcggcaac gtgcgtatgc ggcaggtagt ccagtttctg
     2041 gcgatgagtg aatcgtcggt ttaccgcctg attaaaaaca ccgactttcc ccgccccgtc
     2101 cacctctctt cccgactggt ggtcttcgat gctgctgaaa tccgtcagtg gcaacagcgc
     2161 cgcgccgcca tccgttaatc cacgctcagg gatgagccga ctgatacccc gtcatgaaac
     2221 gcgccccttt tctctgtaag cagtctccgg accgcacgct cgaagtggtg atccttgccg
     2281 gaagcctggc atgggagacc tcacacgtgt ggcgaaaaga tcccgaccgg gaagatgatg
     2341 ttcccccgat ggtactcggc ccggatgaac tggccgatct gagcaatctg accattatca
     2401 ggcccgacac gctctacgtc cgggtacttc gcaccggcga tatcagtgaa gaagagctgc
     2461 tgaaaattgc cgtaaaactg gcgcacgcgg gcgtgcagat ggcccggctg atgacccctg
     2521 acggtgagtt actggagaac tggaccggac agcttgaacg cctgcgacag gaaagacctt
     2581 ctgacatcct gccggatcac ttccgcctcg atgaagaagc actgtggttt gataagctca
     2641 ccgaacggcg cgacggtgaa agcgatgtcc agccccagcg catctgttcc cctctgcgcg
     2701 tcactgccat cacctgcgac agccatgacg gcagctatgg ccgcctgctg gaatggcaca
     2761 ccaccaccgg gcaattgcgg cgctgggcca tgccgatggc gatgctcagc ggcaacggcg
     2821 aggagctgag gcgcattctg ctggagaacg gcctgaccta tatctccacc cgccctgccc
     2881 tgcgcagtct gctgtgcgaa tatatctcgc ggtcactccc cggacgccgg gtcacctgcg
     2941 cggagaaaac cggctggcac aacggcgtgt acgtgctgcc ggatgaggtt atcggtcccg
     3001 atggcgacaa cgtcattctc cagggcagcc actacctgac tggcggcttt gcacaatccg
     3061 gtacgctggc agagtggcag gagcaggtcg ccgcgctctg tgcaggcaat tcacggctgg
     3121 tctttgccgt ctgctgtgcg ctggccgccc cgctgctgcg cctgacagga acgggcggcg
     3181 gaggctttca tctgcgcggt gagtccactg acggtaagac cacggtgatg aaagtggccg
     3241 cttccgtctg tggcgggacg gattactggc actcctggcg ggccaccggg aatgcgctgg
     3301 aaggaattgc cagccgccac aacgatgccc tgctgccact tgatgaactg cgcgaagtgg
     3361 acccgcgtga ggccgggatg attgcctata tgctggcgaa cggacagggc aaggggcgcg
     3421 cccgcaccga tggcgaagtg cgtaaccgca ggcactggac gctgctgctg ttctccaccg
     3481 gggagctgtc gctggcggaa cacaccgaat gcgccgggga gcgcctctac gccgggatgg
     3541 acgtgcgcat ggtacaaatc cccagcgaca ccgggcagca tggttcgttt gagcagttgc
     3601 acgggtttgc cagcggccag cagttcgccg atactctctg cgaccgggtg gcccgcttcc
     3661 acggcaccgc ctttcgtgcc tggctggcct tcctcaccag cgacctggac gccagcacca
     3721 cgctggcgag agagttactt cgccgctacc agaccgccct gatgccggac aacgccggaa
     3781 accaggtgca gcgcatcgtg gcccgttttg ccctgctggc ggtggccggg gagatagcga
     3841 cactgaacgg tatcacgggc tggcaggaag gctcggcgta tggggcggtg cagatctgcc
     3901 ttcacgcctg gctgaacgag cgcggccata ttgccaacca ggaagatgaa ggcgtgctgg
     3961 cgcagatcaa gcggttcatt accgcgcacc agtacagccg ctttgcctcg tgggatggcc
     4021 cggaccgccc gctgaatatg gcaggctttc gccgggtgga aaaagatccg ctgacgggcg
     4081 aggaacacac gctgttcttc atcctgcctg agggctggcg ggaaatatgc cggggattca
     4141 gtcctgccag agcggccaga ttatgccagg aagcggaatg cctgctgccc ggcagcgacg
     4201 ggaaatacca gtcgcaggtt cgcctgccgg agatcggcaa agccagagtc taccgcctca
     4261 cctcccgcat cctcagtatc tgagtgccat ctcgcgtgtc ttaaaaaaag gtggtacagg
     4321 gtgggacaag tggtacacgc agagctggcg cgggtttcag cgtcccactt tagataattt
     4381 ggtttaaaaa aagtggtaca gagtgggaca gccgttgctg aatctgtccc acttttctga
     4441 ctggtatttt tcaggtggta cagcgcaaag ccacggctgg cgcggttgta ccacttgtcc
     4501 cacctgtacc accttaaaaa cagaagaaat gaaaatggcg aaaagggact gcaaaagcaa
     4561 aggctgaaag gaagagagaa taaggggaac ggacttactc ttaaatggat aacctatatt
     4621 tttatattcg taccatttac atgatggagt gcaaagttac cattcaacat gatttggatt
     4681 agatagcaaa attctctata gaatgttttc tagatttcag agggatttaa cgatagctta
     4741 tctcacaaat cacaaatcac aaatcggtca aaaaatatac agaaaaccaa gttttgccca
     4801 gaaatccttt taaaattttt gagttaaaaa tc
//
LOCUS       DAYPHN010000232         4829 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000232 DAYPHN010000000
VERSION     DAYPHN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.232"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..446
                     /gene="espX1"
                     /locus_tag="ACG0BW_004513"
     CDS             <1..446
                     /gene="espX1"
                     /locus_tag="ACG0BW_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFK7261380.1"
                     /translation="GCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTRI
                     KNLRCEDIMPGRWNTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLIQTLVRDNV
                     KLNNDVYKELNNFMLDYDKTSSEMRKYLKSINECMFLMKNIVHQD"
     gene            747..1280
                     /locus_tag="ACG0BW_004514"
     CDS             747..1280
                     /locus_tag="ACG0BW_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261381.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLSAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            1330..2013
                     /locus_tag="ACG0BW_004515"
     CDS             1330..2013
                     /locus_tag="ACG0BW_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFK7261382.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            2026..4476
                     /locus_tag="ACG0BW_004516"
     CDS             2026..4476
                     /locus_tag="ACG0BW_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFK7261383.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGEKVSEYRF
                     DNKQPLPGNYELDVYVNHQWRGKQDITIPESPAKPCLPKVLLTTLGVKTDNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTIPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDDDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            4489..>4829
                     /locus_tag="ACG0BW_004517"
     CDS             4489..>4829
                     /locus_tag="ACG0BW_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFK7261384.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCAVTNVGEQRGTYILKPLSMKGNL
                     TAEKIENPGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKLSYNLEEML"
BASE COUNT         1605 a          945 c          922 g         1357 t
ORIGIN      
        1 ttggttgtgt attatccaat atatcatgtt ttgaaagcga cttatcaaac accatctttt
       61 caaatggaga aataaataat ctcttcatta aaaaaagcaa catatttgga acatcgttca
      121 ctaataccag gataaaaaat ttgcgctgtg aagatattat gcctggtcga tggaacactc
      181 aacttgtcaa taagcactta ggttacagat acacaggagt attcaaaacc ctggcaagta
      241 ttgatgacaa accgtcccgt ttcgaaattt tgataccatt gatacaaact ttagttcgcg
      301 acaatgtaaa gctaaataat gatgtgtata aagaacttaa taattttatg ctcgattacg
      361 acaaaacatc ctcagaaatg agaaaatacc ttaaaagcat caacgagtgt atgttcctta
      421 tgaaaaacat agttcatcaa gattaaaaca ctcccaaaag aagtaatttc agaatgaaat
      481 tatctgtgat gattattaat tcactgcaga gaaacaaaaa ataatctgaa gcatgatctt
      541 aaacatccca ctctgtcact ctaatgatgg caccttgctc ttcttataaa gaagggaata
      601 attaagaata ttaaattttc aaagtttcat caacttcaca tcaagaaatt aaaaaattta
      661 aacattaatc taaataataa caacctgatc acataatcgg gtttgacaac gagtgacctt
      721 ccataccagt taattaagga tgatttatga aaaagtatat tattcccttc gttgcgacaa
      781 cgttgatgtt tagcgcaaca accttcgcca aggatgtcga tcaaggaacg ctaaccatta
      841 gaggagaagt tgttgataca acgtgttact ttgttaatgg tgattcgaca acagatatcg
      901 ttgtcgaaga tgttgtaacc agtgcaatga atcaacttat caataatgaa atctatactt
      961 taaaaaccgg ttcaacctct catgacttag aaattcagtg tgacggcaat acagccccaa
     1021 gtatcgaagt gctagcatca gagtttgatg ctaataactt tacgctgaat aaaggaactg
     1081 ctgagaacat tggctttgcc attttcctcg atgatggaac tttgaccaga atgagaccgg
     1141 ggttaaaaac accgctgaaa gataatggct ctggtcaata ttttcttaat ttaagtgcac
     1201 aatatgcccg cacaagtggc aatcctgtta caaaaggctc cgtagagtca accgtaacga
     1261 ttaaaatcag tgcagattaa cgttttttgc cggtagctct gctaccggta ttatttacgg
     1321 agtgattacg tgagaataat aacagcatta ctgatgtcat tttttatttt acctgttcat
     1381 gccggtgtgg tcatctatgg tacgcgcatt atttatccgg aaaaaaacag agaagttctc
     1441 gtacagttaa tgaatcaaag taaaaatgcc tctttgattc aggcatggat cgatgatggg
     1501 aacaccacta tcgcgccaga aaaaattcag gtcccattta ttctcacacc gccagtaagt
     1561 cgtgtagcag gtggttctgg ccaacaactt aaaataagaa aaatgccaaa caatttgcct
     1621 cacaataaag aaagtttatt ttatctaaat gttctggata tccctccaaa caacccacaa
     1681 aatgcaggaa aaaacaaaat taaacttgca ttgcaaaacc gtataaagtt actttggcgt
     1741 ccaagcggaa tagctccggt agataaaaaa agcctttctc aattaaatat caaaaagaaa
     1801 aataacgcca tctcaataaa caacgaaaca gccaactgga taacggttac aactatcaaa
     1861 gcacaaaacg tgaaagttaa taatgaaagt atcttactgc ctccattttc gaataacgac
     1921 attacattaa aaaataacca cgcatcagaa tatgaactaa cggtagttga tgattacggg
     1981 aataacatcc atagtaaaat cgccgcacga taacaggaaa aagtcatgtt aaggacgaca
     2041 cgatgggtcg ctgctattat ttttttgtat tcttttccgg gttatgctga agaaactttt
     2101 gatacacact ttatgatcgg cggcatgaga ggagaaaaag tttccgaata tcgtttcgat
     2161 aataaacaac ctcttccggg aaactacgaa cttgatgttt atgtaaacca ccagtggcgt
     2221 gggaaacaag atatcacgat cccagagtca ccagccaaac catgtctgcc taaggtgcta
     2281 ctaaccacac tgggggtaaa gaccgataat ctcaatacag aagataattg tattttactg
     2341 gatgaagcgg ttcatggtgg acaatatcag tgggatatca gtgaacaccg actaaattta
     2401 acgatcccac aggcgtatat caatgaactt gaaagaggtt acgttccccc ggaaagttgg
     2461 gatcgcggca ttgatgcttt ttatacctcc tataatttaa gtcagtatcg ctcttatgac
     2521 agcaataata acagtaatac agccagttat ggccggttca acagcggatt aaatttattt
     2581 agttggcagt tacactctga tgccagttat agcaaacctg atgatatgaa aggcaaatgg
     2641 caaagtaata ctctttacct ggaacgtggc tggtcgcaaa tattaagcac agttcagata
     2701 ggagaaaatt acacatcctc actcatattc gactctctgc gatttagcgg aatacgctta
     2761 tttcgcgata tgcaaatgct acctgattct atgcaaagct ttacaccttt agtacaaggt
     2821 gttgcgcaaa gtaacgcctt gataacggtt tcgcaaaatg gctataccat ttaccaaaaa
     2881 gaggttcccc caggaccatt tactatcgca gatttgcaac tttcgggcag cggctctgat
     2941 ctcgatgtca gcattaagga agcagatggt agtgttcgtt cctttttggt gccctactct
     3001 tccgtgccca atatgctgca acctggtgtt tcaaatttcg attttatcgc cggacgcagt
     3061 cagatatatg gtgtcaagaa tcaggaggac tttctcgagg caaattatat atatggcctc
     3121 aacaatcttt tgacacttta tggcggtacg attttatccg ataactataa tgctataact
     3181 ctgggaaatg gttggaatac accgctggga gcaatctctt ttgatgctac ccgatcgagt
     3241 agtaagttaa ataatgatac cagacacgaa ggtacgagct atcaggtcgc ctataataaa
     3301 tatctattgc aaacggctac tcactttagc gtggccgcct ggcgttatgc ttcgcaggat
     3361 tacaggacat tcagcgacca tctttacgaa aatgataaaa taaatcatca gagtgatgat
     3421 gacgattttt atgatattgg cagaaaaaat agcctttctg ccaatatcat gcaaccttta
     3481 tccaataatc tgggaaatgt atcattaagt gcactttggc ggaattactg ggggcgaagt
     3541 ggaaatgcta aagattacca attcagttat tccaatagct ggcaacgcat cagttatact
     3601 ttctctgcca gccaatccta tgatgaaaac gacaaagaag aggagcgttt taatctgttt
     3661 atctccattc ctttctactg gggggatgat attgccaaaa cacgtcacca aattaactta
     3721 tcgaattcga cctcattttc caaagatggc tattcctcca acaatactgg aattactggc
     3781 atagccggtg aacatgatca gttaaattat ggtatatatg ttaatcagca acaacaaaat
     3841 aatgatacct cgcttggtac gaatttaagc tggagaactc ccatcgccac aatagatggc
     3901 agctatagcc attctaaaaa cgcctggcaa agtggtggaa gtattagtag tggattagtt
     3961 gtctggcccg gtggtattaa tataactaac cagttatccg atacatttgc aattctggat
     4021 gcgcctgggt tagaaggcgc gcatattaat ggacaaaaat acaaccgaac aaacagcaaa
     4081 ggccaggttg tttacgacct gatgatacct catcgtgaaa accatctggt acttgatata
     4141 gcaaacagtg aaagtgaaac agaattgcag ggcaatcgtc aaattattgc gccttaccgt
     4201 ggagcagttt cttatgtgca gtttacaact gaccaacgta agccctggta tatacaggca
     4261 ctgcgtcccg atggttcgcc attaaccttt ggctatgacg tactggatct ccaggaaaac
     4321 aatattggag tcgttggcca gggtagtcgc ctttttattc gcgtagatga aattcccact
     4381 ggcatcaaag ttgctctcaa tgatgaacag aacttattct gtactattac ttttcaacac
     4441 gttatcgatg aaaacaaaac atatatatgc cagtaaaagg agtttactat gaagtggctt
     4501 ttattaatca ccttgagtct atattctttt attgtgcaaa gtgctccctg tgcagtgacc
     4561 aatgtcggtg aacaacgtgg aacatatata ctcaagccac tgagtatgaa aggtaatcta
     4621 actgccgaga aaattgaaaa ccccgggaat atcattgagt gtgcagactt gcaagagacg
     4681 accaacgcca ttaaagttca ttttttaaca cagaacgctt taaggtttaa agtcggaagt
     4741 cgtcattaca ttattaattt cccctttgaa tctgacggta aaaaaataga gcttccaggg
     4801 aagttatctt ataatttaga agaaatgct
//
LOCUS       DAYPHN010000233         4774 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000233 DAYPHN010000000
VERSION     DAYPHN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.233"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            107..1081
                     /gene="hemB"
                     /locus_tag="ACG0BW_004518"
     CDS             107..1081
                     /gene="hemB"
                     /locus_tag="ACG0BW_004518"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFK7261385.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRGRTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1121..1972)
                     /gene="tauD"
                     /locus_tag="ACG0BW_004519"
     CDS             complement(1121..1972)
                     /gene="tauD"
                     /locus_tag="ACG0BW_004519"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFK7261386.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLREQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(1969..2796)
                     /gene="tauC"
                     /locus_tag="ACG0BW_004520"
     CDS             complement(1969..2796)
                     /gene="tauC"
                     /locus_tag="ACG0BW_004520"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFK7261387.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2793..3560)
                     /gene="tauB"
                     /locus_tag="ACG0BW_004521"
     CDS             complement(2793..3560)
                     /gene="tauB"
                     /locus_tag="ACG0BW_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFK7261388.1"
                     /translation="MLQISHLYADYGGKLALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVAFGLQLAGVEK
                     ALRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3573..4535)
                     /gene="tauA"
                     /locus_tag="ACG0BW_004522"
     CDS             complement(3573..4535)
                     /gene="tauA"
                     /locus_tag="ACG0BW_004522"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFK7261389.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1101 a         1380 c         1215 g         1078 t
ORIGIN      
        1 ctctctttca ttctcttctt gtcaacatcg cgacaacttt cgtaaaacat ccccaccctg
       61 cttcaggtat actatgcccc tcgattccac aaacatcagg cagaccatga cagacttaat
      121 ccaacgccct cgtcgcctgc gcaaatctcc tgcgctgcgc gctatgtttg aagagacaac
      181 acttagcctt aacgacctgg tgttgccgat ctttgttgaa gaagaaattg acgactacaa
      241 agccgttgaa gccatgccag gcgtgatgcg cattcctgag aaacatctgg cacgcgaaat
      301 tgaacgcatc gccaacgccg gtattcgttc cgtgatgact ttcggcatct ctcaccatac
      361 cgatgaaacc ggcagcgatg cctggcggga agatggactg gtggcgcgga tgtcgcgcat
      421 ctgcaagcag accgtgcctg aaatgatcgt gatgtcagac acctgctttt gtgaatacac
      481 ttctcacggt cactgcggtg tgctgtgcga gcatggcgtc gacaacgacg cgactctgga
      541 aaatttaggc aagcaagccg tggttgcagc tgctgcaggt gcggacttca tcgccccttc
      601 cgccgcgatg gatggtcagg tacaggcgat tcgccaggcg ctggacgctg cgggctttaa
      661 agatacggcg attatgtcgt attcgaccaa gtttgcctct tccttttatg gcccgttccg
      721 cgaagcggca ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga acccaatgaa
      781 ccgtcgtgag gcgattcgtg aatcattgct ggatgaagcc cagggcgcag actgcctgat
      841 ggtgaaacca gctggagcgt acctcgacat cgtgcgtgag ctgcgtggac gtactgaatt
      901 gccgattggc gcgtatcagg tgagcggtga gtatgcgatg attaagttcg ccgcgctggc
      961 gggtgctata gatgaagaga aagtcgtgct cgaaagctta ggttcgatta agcgtgcggg
     1021 tgcggatctg attttcagct actttgcgct ggatttggct gagaagaaga ttctgcgtta
     1081 atgatcatta tgccgggtta tccaacccgg catttccccc ttaccccgcc cgataaaacg
     1141 gtttatcccc aaggattgtc gcccgatgca ttatccgccg ctgtggcagg taatccgcat
     1201 tggcatagtg ctgagtcacg cggttatccc aaatcgcaat atcatttggt tgccagcgcc
     1261 agcgcacctg aaactccggt ttggtgatat gggcaaacaa aaaacttaac aaggcttcgc
     1321 tctctttctc gctgacatca acaattcgcg tagtaaagcc ttcattcaca aacagcgcct
     1381 gtttaccgct caccggatgc gttcgcacca ccggatgtag taacggcgga tttttcgcga
     1441 ccgcctcgcg ccagcgttga tgctcctcct ccgttttgcg gtatttgtat tctgggaacg
     1501 atttacggaa atcatgctcc gcacgcagcc cgctcaacag ctggcggaag ggaacagaga
     1561 gtgcctcata cgccgcaata ccgctggtcc atagcgtatc gccgccagtc gagggtaact
     1621 ctttcgccgc cagaatcgcc ccagcaggag gcgtttcaat aaatgtcaca tcggtatgcc
     1681 agttgtcgtt atctggcgga ttatcgttat gagtatccag tacgataatc tcgtcaaccc
     1741 cttcggcatg agggtaaaca ggatgaatat gcagctcgcc aaaacgctgg gccagcgcgc
     1801 gttgctgctg cggcgtaatg gcctgttcac gcagaaacac cacctgatga cgcagtaccg
     1861 catggtaaag ctgttcaaac tggttatcac ttaacgggcg cgtcaggtcg gcacccgtaa
     1921 tttgtgcgcc aatatacggc cccagcgggg taatgctcag acgttcactc attgtacttc
     1981 tccatgccag ggcgtgaggc ggcgctgtaa cgcgcgcaga cccagttcta aaagaaaggc
     2041 gataatcgca atcaccgcga tccccgccag caccacgtca gtcgcgagaa attcacccgc
     2101 tgactggacc ataaaaccca aaccgcgcgt cgcggcaatc agctccgccg ccaccagggt
     2161 agaccagccc acacccagac caatgcgtaa tccggtgaga atttccggca gcgcaccggg
     2221 caaaataaca aaccacagca cctgcgcacg gctggcaccc agcgactggg cggcacgaat
     2281 gcgaacctgc tgcgcgcttt tcacccccgc cagcgccgac atcgccacag gtgcaaaaat
     2341 cgctaaatag atcagtaaga tcttcgaggt ttcaccaata ccaaaccaga tcaccatcag
     2401 cggcaaatac gccagcggcg gcaccgggcg atacaattca attatcggat ccagaatgcc
     2461 gcgcaccgtc gggcttagcc ccatggcgat cccaaccgga atgccgatca acaccgccgc
     2521 aagtagcgcc agcacaatgc gcgtcaggct ggctgccaga tgctgccaca gggtggcgtc
     2581 cataaatccc tgcggtccgg caatggtgag cagttttgcc agtacttgtt gcggcggcgg
     2641 caaaaacagc gggctaatca gttgcagcgc cgccaccgtc caccatacag tgagtaaaac
     2701 cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc atttcagccg
     2761 ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg ttgctcaaat
     2821 acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt gatgctgcgg
     2881 ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc cagcacacgg
     2941 cctgggccgg gtgaaagcag aaccagttcg gttgccataa acaccgcttc ttctatatcg
     3001 tgggtaatca acagcacctg cttacctgtc tcctgccaga gtttcagcag cagagtttgc
     3061 atctgatcgc gggtgaaggc gtccagcgcg ccaaacggtt cgtcgagtaa taacagctgg
     3121 ggattcgccg ccagcgcgcg ggcaatccct acccgctgac gttgaccgcc ggaaagctgc
     3181 cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg gtgcgcgatt
     3241 tccaggcgca gtgctttctc tacacctgcc agttgcaggc cgaacgccac gttgtctacc
     3301 acattacgcc acggcaacag cccttcattc tgaaaaacca cgccccgctc tgctcccggt
     3361 ccctcaatgc acttacccgc cagttgaatg ctgccatgct gataaggcac aaaaccggca
     3421 atcagattca gcaaggtggt tttgccgcag ccagacggcc ccagcaccac cagcagctcg
     3481 ccgctttcca gcgtcaggtt gatatcttcc aacgccagtt tgccgccata atcggcatag
     3541 agatgagaga tttgcagcat ccgcgcctcc ttttattgta cgaagcgcga ggtaacgtac
     3601 tggctgtaat cattcgccac ggcggggact ttgccctgct ctttcaaaaa ctgcgcggtg
     3661 tcgataattg ctttgttcac cggtccggtc agttctgccg tttgttgctg cggcgtcaga
     3721 taggtattcc ccttcaccag ccccggcacg tcaccttccg gcacgccgct taaacgcgcc
     3781 agcttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat gtacggttgc
     3841 tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt ctcggcaaaa
     3901 tctttgcgca ctacccaaac atccagcgtc ggcgcgcccc actgcccgac ctgttcagaa
     3961 tcggtcagca ccttgccgtc tttttccagg gcgttaaccg ccggtgccca gacataagca
     4021 ccatcaatgt ctccccgttg ccatgcggcg ataatcgcgg gtggctgcag gttcactatc
     4081 tccacttgtc cgggttttat accccagtgt ttcagcgccg ccagcaggct gtaatgggtg
     4141 gtggagataa acggtacggc gatgcgtttg ccaatcagat cttccggttt gctgatagtt
     4201 ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagtaagaa gacttcaatc
     4261 ggcacctgtt ggctggctgc caccgctaat gggctggaac cgaggttacc gatttggaca
     4321 tcgcctgaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg ccagtccacc
     4381 gtcgccccgc tctctttagc aaaggtgtta tcagcctgag ccactttcgc cggttcagcg
     4441 gaggtttgat aggccacggt gacgttcacc gcctgtgcct gaaaagcgat gaatgccagt
     4501 gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc tgtcttgtta
     4561 taggagcagt attcaggaat aaaaacattc attaaaagaa ttagtcgtta tcgcacagat
     4621 gattttattc ttagcaaaaa agcggcaaag gaaagattaa atatgcaatt acacccacgc
     4681 tgacaattca gtaagttact gcacttcatt tttattaatg cataaacaag cagaacataa
     4741 tgtcttttat tttattaaca gcaatacata taat
//
LOCUS       DAYPHN010000234         4769 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000234 DAYPHN010000000
VERSION     DAYPHN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.234"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(116..841)
                     /gene="phoU"
                     /locus_tag="ACG0BW_004523"
     CDS             complement(116..841)
                     /gene="phoU"
                     /locus_tag="ACG0BW_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFK7261390.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(856..1629)
                     /gene="pstB"
                     /locus_tag="ACG0BW_004524"
     CDS             complement(856..1629)
                     /gene="pstB"
                     /locus_tag="ACG0BW_004524"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFK7261391.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1720..2610)
                     /gene="pstA"
                     /locus_tag="ACG0BW_004525"
     CDS             complement(1720..2610)
                     /gene="pstA"
                     /locus_tag="ACG0BW_004525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFK7261392.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2610..3569)
                     /gene="pstC"
                     /locus_tag="ACG0BW_004526"
     CDS             complement(2610..3569)
                     /gene="pstC"
                     /locus_tag="ACG0BW_004526"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFK7261393.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3657..4697)
                     /gene="pstS"
                     /locus_tag="ACG0BW_004527"
     CDS             complement(3657..4697)
                     /gene="pstS"
                     /locus_tag="ACG0BW_004527"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFK7261394.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAITKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
BASE COUNT         1136 a         1322 c         1217 g         1094 t
ORIGIN      
        1 gagagtttcg aggtgagttt gagtgcaggg agaaagtgta tcagcccggt aactatgtat
       61 gaccgggctg attgccagat tcggcgtaaa agccttatct gacctacagt tagcgttact
      121 ctttcgggtc ttttccggcc agcagtttat ccagctcatc accgcctaca tgacggaaat
      181 cctgcccctt cacgtagtag aagataaact cacaaatatt ctggcagcgg tcgccgatac
      241 gttcgattga acgcgcacag aatagcgcgg taagtacgct cggaatggtg cgcgaatctt
      301 ccatcatgta ggtcatcagt tgacgaacaa taccttcgta ctcctgatcg acttttttat
      361 cttcacgata aatacgtacc gcttcgtcaa tgtccatccg cgcgaacgcg tccagcacgt
      421 cgtgcagcat ctggatggta tgacggccca gcgactccag acttaccagc aacggctgat
      481 gctgctggga gaatttctcc agcgcagtac ggcagatttt gtccgccacg tcgccaatac
      541 gctccagctc ggcaatggtt ttactgatca ccataaccag acgcaggtcg ctcgccgtcg
      601 gctgacgttt ggcgataatg cgcacgcagg cttcatcgat cgccacttcc atcatgttga
      661 cgttcttgtc gccttcgatg acgcgcttcg ccagatcgct gtcctggtta tgcatcgcgg
      721 tgattgcatc agaaagctgc tgctccacca tgccgcccat ggtcatcacc tgcgtgcgga
      781 tgctttccag ttcggcgttg aactggccgg aaatatgttt attaagattg agactgtcca
      841 taacgcactc ctgaatcaac cgtaacgacc ggtgatgtag tcttctgttt gtttcttcgc
      901 tggcttggtg aacagatcgt ccgtgttgct gaactcaatc aattcgccca ggtacataaa
      961 cgccgtgtgg tcggaacaac gcgcagcctg ctgcatgttg tgggtgacga tcactacggt
     1021 gtaatcctgc ttcagttcgg tgatcagctc ttcaatacgc ccggtagaga tagggtcgag
     1081 cgccgaacac ggttcgtcga gcagcagcac ttccgggcga atggcgatac cacgcgcaat
     1141 gcacagacgc tgttgctgac caccagagag agagtaaccg ctctgatgca atttatcttt
     1201 ggtttcgttc cacaatgcgg ctttggtcaa tgcccactgc acgcgctcgt ccatgtcggc
     1261 acgggagagc ttctcaaaca gacgcacacc aaaggcgatg ttgtcgtaga tggacatcgg
     1321 gaacggcgtc ggtttctgga acaccatgcc cactttcgca cgcagcaggg cgatatcctg
     1381 agagttggtc aggatgttgt cgccatcgag cagaatttca ccttccgcac gctgttccgg
     1441 gtacagttca aacattttat tgaaggtacg cagcagcgtc gatttaccgc agccggacgg
     1501 cccgataaac gccgttacct ggtttttagc gatatccagg ttgatgtttt tcagggcatg
     1561 gaatttgccg tagtagaagt tcaaattacg aacctgaatt ttactcgggg cagtttcaac
     1621 catactcatt gcaatctttt cctcatcgtg cctgatgcac ttcgctaatc aggcttaccg
     1681 taggccggat caggcgttca cgccgcatcc ggcgaagtgt taaccgtgtt tattcttcgc
     1741 aaaaacaacg cgcgccagaa tgttcagcag cagtacgcac agggtaatga tcaacacccc
     1801 ggcccaggcc agttgctgcc attccgcaaa cggactcatc gcaaacttaa agatcgtcac
     1861 cggcaggttg gcgatcggct gcatcatgtc cgtgctccag aactggttgg aaagcgcggt
     1921 aaacagcagc ggcgcggttt caccagcaat acgggcaatc gccagcagga taccggtcat
     1981 aatcccggac accgacgctt tcagcgtgat cgcggagatc atcttccact tcggtgtacc
     2041 cagcgcataa gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt tctcggtggt
     2101 gcggataaca atcggcacct gcaacagcgc cagcgcaatc acgcccgccc agccggagaa
     2161 gtgctccatc tgcgccacca caatggtgta aacaaacaga ccaaccacaa tcgacggtgc
     2221 ggagagcaga atgtcgttaa tgaagcgaat cacttctgcc agccaggatt tacgaccata
     2281 ttcagccaga taaatccccg ccataatgcc caacggcgta ccgaataccg tggcccacaa
     2341 aattaacagc ccgctacccg ccagggcgtt cgccagacca ccaccttccg tattgggcgg
     2401 cggcgtcatt tcagtgaaca gcgccagcga cataccatcg ataccgcggg tgatggtgga
     2461 cattaaaatc cagatcagcc agaacaggcc gaaggccatc gtcgccatcg agagcgtcag
     2521 cgcaatacgg ttcttaaggc ggcgacgcgc ctgcatttta cggcgggatt ccgccagcgc
     2581 cgcagtggtt tgcatttcaa ccatagccat tagcgtgccc cctcattctt agccaggcgc
     2641 ataatcataa acttcgatgc ggcgaggacg atgaaggtaa tcacaaacag gatcaggccc
     2701 agttccatca gtgcggcaac gtgcagaccg gattccgctt cggcaaattc gttcgccagc
     2761 gcagaggtga tactgttgcc cggcatgtac agcgaggcgc tgtcgagctg gtaggtgtta
     2821 ccgatgataa aggtcaccgc catggtttca ccgagcgcac gccccagacc cagcataatg
     2881 ccgccgataa caccattttt ggtgaacgga agaacgatac gccagataac ttcccaggtg
     2941 gtgcagccga taccgtaggc cgactctttc atcatcaccg gggtttgttc gaatacatcg
     3001 cgcattaccg ccgcaatgta cggaataatc atgatggcga ggatcacgcc tgccgcgagg
     3061 ataccgatac caaaggccgg gccggagaac agcgcgccga caatcgggat attcgacatg
     3121 atattgccga ccggctcctg gaagtaaacg gcgaacagcg gcgcaaagat aaacaggccc
     3181 cacatgccgt aaacgatact tggaatggct gccagcagct caatggcgat acccagcggg
     3241 cgcttcagcc agccaggagc aagctcggtc aggaacaggg cgataccgaa actcactgga
     3301 acggcgatca gcagcgcgat aaacgaggtc accagcgtac cgtagatcgg caccagcgcc
     3361 ccgtagatat cgttcggcgc atcccactct ttggtccaca ggaaagccag accaaatttc
     3421 tgaatgctcg gccaggagga gatgatcaga gagacaataa tgccacccaa catcaatagc
     3481 acaatcagcg ccgccagttt taccagcacg ctgaaaatta tgtcgccctt tttacccggt
     3541 gggttaaaag caggcttggt tgcagccata agttactctt cagttaaacg cgtttacaaa
     3601 gtcattttgt aatgccggat gcggcgtaaa aaataccgta cccggcctga gttttattag
     3661 tacagcggct tgccgctact gtctttaata ttggtcttcc acgcagcgcg aacctgttcc
     3721 actacgctat ccggcaggct ggcgtaatcc aggtcgttcg cctgtttagc cccggttttg
     3781 tatgcccagt cgaagaactt cagcacttct gtgccttgtt ctggtttctt ctgatctttg
     3841 tggatcagaa tgaacgtggt agaggtaata ggccacgcat cttcgccttt ctggttggtc
     3901 agatcctgag cgaaggtttt gctccagtct gcgccttttg ctgcattagc gaagttttct
     3961 tcggtcggca aaacaggttt accatcagcg gagatcaatt tggtatacgc caggttgttc
     4021 tgcttagcgt aagcatattc aacgtaacca attgcacctg gcagacgctg aacgaacgcg
     4081 gcgataccgt cgttaccttt accgcccaga ccaatcggcc attttacggt agatcccgta
     4141 ccaacgttgt ttttccactc ttcgttcact ttcgccaggt agctggtgaa gacgaaggac
     4201 gtcccggagc catctgcgcg gcgtactaca gcaatgttct gtgacggcag ttttaagcct
     4261 ggattcagtt tggtgatggc ttcatcatcc cacttcttga ttttgccgag gtagatgtca
     4321 ccaagggttt taccatccag caccagctcg ccagacttca accctggaat gttaaccgcc
     4381 agtaccacgc cgccaatcac ggtcgggaac tggaacaaac cttcctgagc cagtttttcg
     4441 tcagacagcg gcgcgtcaga ggcaccaaaa tcaacggtat tagcgataat ctgttttacg
     4501 ccaccggaag aaccgatacc ctggtagtta actttattac cggtttcttt ctggtaagtg
     4561 tcagcccatt tggcatacac cggcgcaggg aaggttgcac ctgcgcctgt caggcttgct
     4621 tctgcaaaca cagagaaagc actcatcgat aaggtcgcgg cgacaacagt tgcgacggtg
     4681 gtacgcataa ctttcataat gtctcctggg aggattcata aagcattgtt tgttggctac
     4741 gagaagcaaa ataggacaaa caggtgaca
//
LOCUS       DAYPHN010000235         4705 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000235 DAYPHN010000000
VERSION     DAYPHN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.235"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..448
                     /locus_tag="ACG0BW_004528"
     CDS             <1..448
                     /locus_tag="ACG0BW_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HFK7261395.1"
                     /translation="ISEKEQILKQLHIVQEQLEHYFIENQEIKKKLPPVLYGAAEQIK
                     QELGYRLGYIIVSYSKSLKGIITMPFALIRECVFEKKRKKSYGVDVPLYLYADADKAE
                     RVKKHLSYQLGQAIISSANSIFGFITLPFKLIVVVYKYRRAKIKGC"
     gene            complement(545..1579)
                     /locus_tag="ACG0BW_004529"
                     /pseudo
     CDS             complement(545..1579)
                     /locus_tag="ACG0BW_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323773.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            1740..3302
                     /gene="kfiC"
                     /locus_tag="ACG0BW_004530"
     CDS             1740..3302
                     /gene="kfiC"
                     /locus_tag="ACG0BW_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267195202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis
                     glycosyltransferase KfiC"
                     /protein_id="HFK7261396.1"
                     /translation="MNAEYINLVERKKKLGTNIGALDFLLSIHKEKVDLQHKNSPLKG
                     NDNLIHKRINEYDNVLELSKNVSAQNSGNEFSYLLGYADSLRKVGMLDTYIKIVCYLT
                     IQSRYFKNGERVKLFEHISNALRYSRSDFLINLIFERYIEYINHLKLSPKQKDFYFCT
                     KFSKFHDYTKNGYKYLAFDNQADAGYGLTLLLNANDDMQDSYNLLPEQELFICNAVID
                     NMNIYRSQFNKCLRKYDLSEITDIYPNKIILQGIKFDKKKNVYGKDLVSIIMSVFNSE
                     DTIAYSLHSLLNQTYENIEILVCDDCSSDKSLEIIKSIAYSDSRVKVYSSRKNQGPYN
                     IRNELIKKAHGNFITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNGKIQF
                     FYDDKATRMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRLLQPL
                     ILGLWGDSGLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGLLSRY
                     GLFKDVSGIIEQ"
     gene            3344..4522
                     /gene="kfiD"
                     /locus_tag="ACG0BW_004531"
     CDS             3344..4522
                     /gene="kfiD"
                     /locus_tag="ACG0BW_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis UDP-glucose
                     dehydrogenase KfiD"
                     /protein_id="HFK7261397.1"
                     /translation="MFGTLKITVSGAGYVGLSNGILMAQNHEVVAFDTHQKKVDLLND
                     KLSPIEDKEIENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGSNYFDTSSVE
                     AVIRDVTEINPNAIMVVKSTVPVGFTKTIKEHLGINNIIFSPEFLREGRALYDNLHPS
                     RIIIGECSERAERLAVLFQEGAIKQNIPVLFTDSTEAEAIKLFSNTYLAMRVAFFNEL
                     DSYAESFGLNTRQIIDGVCLDPRIGNYYNNPSFGYGGYCLPKDTKQLLANYQSVPNKL
                     ISAIVDANRTRKDFITNVILKHRPQVVGVYRLIMKSGSDNFRDSSILGIIKRIKKKGV
                     KVIIYEPLISGDTFFNSPLERELAIFKGKADIIITNRMSEELNDVVDKVYSRDLFKCD
                     "
BASE COUNT         1498 a          737 c          901 g         1569 t
ORIGIN      
        1 aatatcagaa aaagaacaga ttttaaaaca attgcatata gtgcaagagc agttggaaca
       61 ctattttata gaaaatcagg aaattaaaaa gaaacttcca cctgtgctat atggagcagc
      121 tgagcagata aaacaagagt taggttatcg acttggttat attatagtct cgtattctaa
      181 atccctcaag gggattatta ccatgccatt tgcacttatc cgtgagtgtg tttttgaaaa
      241 aaaacgtaag aagagttatg gcgttgatgt gccactctat ttatatgctg atgctgataa
      301 ggctgaaaga gttaagaaac atttatctta tcaattaggg caggctatta tctccagtgc
      361 taattcgata tttggattca ttacccttcc atttaagtta attgttgttg tttataaata
      421 taggagagct aaaatcaagg gctgttaaaa atgtgaaccc taatgagata tattgcaaat
      481 tttatttttc tctttgtggt gttttgcttt cgttaaaata gttagttatt ttatttatat
      541 aatatcacgc attataatac caatttatac ttttgcaagt gaccgtatag attcgccaca
      601 tattgcaaat tttgttctct cgtaaaatat tttcttctgg tgtcagtaat tcgagcactt
      661 catgctgtct tttactgcag tatagtacta ggttttcagc tagtttctga ttaaatatgt
      721 ttagctcttt aaagagtcta tttatttaaa ttcaatgaac tgttctttcg gcgttcgctt
      781 aaaacgttca gaggtgcgtt ctaggcgtaa aggggtagat ccatagggct tgctagcgga
      841 ttcctgcggt gctttgtcga agtttcctgg gaactctttc ccgttatctg cgatgccctg
      901 gttgatatcg atagggaata gccggtctgc acggcaaaag aagagtttga tgatatcaca
      961 gttaagtgac ggcacggcct gtgccagagc gtagttgctg tgttcgtcga tcatggtaat
     1021 gacataacag cgcagctcgc ccattctgag ctcaatagca tctataccaa cgagctcact
     1081 gctctttacc gggcgatagc gttttgatct gcgggttgga ggattgtatt ttttatgaac
     1141 agtgtttttc cttggggagg cagtcacatc ggtatcagtc gcattttatc gtgtgcggcc
     1201 gtgaacatcc tgccgatggt tgaaatgctc ggacagacca ggcggtgctg ttcactccac
     1261 ggcttcaagc aaacaaaaat ctgttcttct aggttgggaa gctctattct cagtcgacgt
     1321 atttcttaca gaattacgag atgccattgc tttgtgcgat ttactagcgg agctttgcta
     1381 cgcgaaatag gtgcctctgg gccataatac agcgttcgtg tggatacagc aaaaacttcc
     1441 gcaactgtat tgatctcatg tttctcccag aagtatattt tttcatcctt aattttgtaa
     1501 tctcaggtat aacaaagtgt ttcatcacat agatgttggc atggtaatgc ctcaaatatc
     1561 cgccgcagat acgttgcatc aacttagcat ttccctcgct tgtccggaga taattgcaat
     1621 atctctgtga gcttacactg tgacattcgt tgagttttag tgatgttttt aaagatttat
     1681 atttataata tttagtaaat gcagttttat tctcatttta tttatcatta agtgaatgta
     1741 tgaacgcaga atatataaat ttagttgaac gtaaaaagaa attagggaca aatattggtg
     1801 ctcttgattt tttattatca attcataagg agaaagttga tcttcaacat aaaaactcgc
     1861 ctttaaaagg taacgataac cttattcaca aaagaataaa cgaatacgac aatgtacttg
     1921 aactatctaa gaatgtatca gctcagaatt ctggcaatga gttttcttat ttattgggat
     1981 atgcagattc tcttagaaaa gttggtatgt tggatactta tattaaaatt gtttgttatc
     2041 taacaattca atctcgttat tttaaaaatg gcgaacgagt taagcttttt gaacatataa
     2101 gtaacgctct acggtattca aggagtgatt ttctcattaa tcttattttt gaacgatata
     2161 tcgaatatat aaaccatcta aaattgtcgc ccaaacaaaa agatttttat ttttgtacga
     2221 agttttcaaa atttcatgat tatactaaaa atggatataa atatttagca tttgataatc
     2281 aagccgatgc agggtatggc ctgactttat tattaaatgc aaacgatgat atgcaagata
     2341 gttataatct actccctgag caagaacttt ttatttgtaa tgctgtaata gataatatga
     2401 atatttatag gagtcaattt aacaaatgtc tacgaaaata cgatttatca gaaataactg
     2461 atatataccc aaataaaatt atattgcaag gaattaagtt cgataagaaa aaaaatgttt
     2521 atggaaaaga tcttgttagt ataataatgt cagtattcaa ttcagaagat actattgcat
     2581 actcattaca ttcattgttg aatcaaacat atgaaaatat tgaaattctc gtgtgcgatg
     2641 attgttcatc ggacaaaagc cttgaaataa ttaagagcat agcttattct gattcaagag
     2701 tgaaagtata tagctcacga aaaaaccaag gcccttataa tataagaaat gagctaataa
     2761 aaaaagcaca cggtaatttc atcacctttc aagatgcaga tgatctttct catccggaga
     2821 gaatacaaag acaagttgag gttcttcgca ataataaggc tgtaatctgt atggctaact
     2881 ggatccgtgt tgcgtcaaat ggaaaaattc aattcttcta tgatgataaa gccacaagaa
     2941 tgtctgttgt atcgtcaatg ataaaaaaag atatttttgc gacagttggt ggctatagac
     3001 aatctttaat tggtgcagat acggagtttt atgaaacagt aataatgcgt tatgggcgag
     3061 aaagtattgt aagattactg cagccattga tattggggtt atggggagac tccggactta
     3121 ccaggaataa aggaacagaa gctctacctg atggatatat atcacaatct cgaagagaat
     3181 atagtgatat cgcggcaaga caacgagtgt tagggaaaag tatcgtaagt gataaagatg
     3241 tacgtggttt attatctcgc tatggtttgt ttaaagatgt atcaggaata attgaacaat
     3301 agtttgttat tctatatata ttaaattttt ggggctatat aaaatgttcg gaacactaaa
     3361 aataactgtt tcaggcgctg gttacgttgg gctttcaaat ggaattctaa tggctcaaaa
     3421 tcatgaagtg gttgcatttg atacccatca aaaaaaagtt gacttactta atgataaact
     3481 ctctcctata gaggataagg aaattgaaaa ttatctttca actaaaatac ttaattttcg
     3541 cgcaactact aacaaatatg aagcctataa aaatgccaat tacgttatta ttgctacacc
     3601 aacgaattat gacccaggtt caaattactt tgatacatca agcgttgaag ctgtcattcg
     3661 tgacgtaacg gaaatcaacc caaacgcaat tatggtggtt aaatctacgg tcccagtagg
     3721 tttcacaaaa acaattaaag aacatttagg tattaataat attatcttct ctccagaatt
     3781 tttacgagaa ggaagagccc tatacgataa tctccatcca tctcgcatta ttatcggtga
     3841 atgttctgaa cgggcagaac gtttggcagt gttatttcag gaaggagcga ttaaacaaaa
     3901 tatacccgtt ttatttacag attctacgga agcggaagcg attaagttat tttcaaatac
     3961 ttatttggct atgcgagttg cattttttaa tgaattggat agttacgcag aaagttttgg
     4021 tctgaatacg cgtcagatta ttgacggtgt ttgtttggat ccgcgcattg gtaattacta
     4081 caataatcct tcttttggtt atggtggcta ctgtttgcca aaagatacca agcaattatt
     4141 agccaactat cagtctgttc cgaataaact tatatctgca attgttgatg ctaaccgtac
     4201 acgtaaggac tttatcacta atgttatttt gaaacataga ccacaagttg tgggggttta
     4261 tcgtttgatt atgaaaagtg gttcagataa ttttagagat tcttctattc ttggtattat
     4321 aaagcgtatc aagaaaaaag gcgtgaaagt aattatttat gagccgctta tttctggaga
     4381 tacattcttt aactcacctt tggaacggga gctggcgatc tttaaaggga aagctgatat
     4441 tattatcact aaccgaatgt cagaggagtt gaacgatgtg gtcgacaaag tctatagtcg
     4501 cgatttgttt aaatgtgact aatgtattgt tatatactat taactattaa gagaaggaaa
     4561 tgcattattt aatccgttaa aaatatgcct cgttggtatg ttctttatta atcctcgatc
     4621 gtaaaataag atgttggtta aatttcttgc cctgctgttg tgtttatatc gaatttttca
     4681 taactctaat gttaattaga atact
//
LOCUS       DAYPHN010000236         4646 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000236 DAYPHN010000000
VERSION     DAYPHN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.236"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..2014)
                     /locus_tag="ACG0BW_004532"
     CDS             complement(<1..2014)
                     /locus_tag="ACG0BW_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFK7261398.1"
                     /translation="MAAVKISGVLKDGAGKPIQNCTIQLRAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPE
                     ALRRFEAMVEEVARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAAASQ
                     TASANSATAAKKSETNAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQVA
                     AAQSESAAAGSATSAAGSATAAANSQKAAKTSETNAKSSQTAAKTSETNAKASETAAK
                     NSQDAAAESESVAAGSASAAAASATASANSQKAAKTSETNAKTSETAAANSAKASAAS
                     QTAAKASEDAAREYASQAAEPYKQVLQPLPDVWIPFNDSMDMIAGFGPGYKKITVRDD
                     VVTIPSDKIVPFSRASSATYINKSGALTIAEINEPRFEKEGLLIEGQRTNYLKNSNTP
                     ELWYYHSLLAKEYGVDNYGFNYATVTVTDSLVGTTSSYQIHSVTGENRFSLSIGETCT
                     FSARFRGVNSRCRVHISLVIGENVKTSVDVCLEPDTGEVKAYGNKALVTFRTEKQGDW
                     CYFETTYTAAENIDEVVCGFFITNPEDGGFSSDSSLTMTTPQIEKGPCASSFIISGNT
                     PTTRASDMVIFSTRNNISSVPLSCLVEIHKNWDSTPNQAPRILTTIG"
     gene            complement(2079..2678)
                     /locus_tag="ACG0BW_004533"
     CDS             complement(2079..2678)
                     /locus_tag="ACG0BW_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFK7261399.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(2746..>4646)
                     /locus_tag="ACG0BW_004534"
     CDS             complement(2746..>4646)
                     /locus_tag="ACG0BW_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFK7261400.1"
                     /translation="GNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLF
                     RCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVT
                     ADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSELLVSTARTAETTYRFRQLALGN
                     YRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIKLTPGYFQITATPHLAVYDPTVQFE
                     FWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEA
                     VGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQT
                     VNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGM
                     QSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSL
                     TPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPR
                     DSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNG
                     AEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLVVVMKK
                     ATAGISIS"
BASE COUNT          985 a         1310 c         1113 g         1238 t
ORIGIN      
        1 cgccaatagt ggttaaaatt cgtggggcct gatttggcgt agagtcccaa tttttatgta
       61 tttcaacaag acagcttaaa gggacagaac ttatgttatt tctggttgaa aaaataacca
      121 tatcactggc gcgcgtggtt ggtgtgtttc cggaaataat aaacgatgaa gcgcatggcc
      181 ctttttcaat ttgcggcgta gtcattgtta atgacgaatc agaagaaaaa ccaccatctt
      241 caggattggt tataaaaaaa ccgcatacca cttcgtcaat attctctgcg gcggtatatg
      301 ttgtttcaaa gtaacaccag tccccctgtt tttccgttct gaatgtcacc agcgccttat
      361 taccgtacgc cttaacctca cctgtatctg gctccagaca tacatcaacg gatgttttaa
      421 cattttcgcc aataacaaga gatatatgga cgcgacaacg tgaattaact ccacgaaagc
      481 gggcagaaaa agtgcaggtt tcaccgatgg acagagaaaa cctgttctct ccagttacac
      541 tatgaatctg ataactactg gttgtgccta caagactgtc tgtcacggtg acggttgcat
      601 aattaaaacc gtaattatca accccatact ctttcgccag taaactgtgg tagtaccaca
      661 attccggtgt attagaattt tttagatagt tagttctctg cccttcaatt aacagccctt
      721 ctttttcaaa acgtggctca ttaatttctg caatggtaag agcgccggac ttattgatat
      781 aggtcgcgga actcgcgcgg gaaaacggga caattttgtc ggatggtata gtgaccacat
      841 catcgcgcac tgttattttt ttatatcccg ggccaaatcc tgcaatcata tccatcgagt
      901 cattaaacgg tatccacaca tcaggcagtg gctgcaaaac ttgtttatac ggctcagctg
      961 cctggcttgc gtactccctg gctgcatctt cacttgcttt agccgctgtc tggcttgcag
     1021 cggatgcttt cgccgagtta gctgccgcag tctcgcttgt ctttgcgttg gtttcactgg
     1081 tttttgctgc tttttgactg ttagctgatg cagtggcaga agcagccgcc gcgcttgcag
     1141 aaccagctgc aacactctcg ctttcggctg ctgcatcctg gctgtttttc gccgcagttt
     1201 cgctggcttt ggcattcgtt tcgctggtct tcgctgccgt ctggctggac tttgcgttag
     1261 tttcactcgt cttcgcagct ttctggctgt tagccgcagc agttgctgat ccggctgctg
     1321 aagtcgcaga accggctgcc gcgctctcgc tttgggctgc tgcaacctgg ctgttttttg
     1381 ccgcagtttc actggctttg gcattcgttt cgctggtttt cgctgccgtc tggctggact
     1441 ttgcgttggt ttcgctcgtc tttgcggctg tctcgctatt tttcgcgttg gtttctgatt
     1501 ttttggctgc tgtcgcggag tttgccgatg cagtctgtga ggccgctgcc gcctgtgcac
     1561 tattagctgc attcgtttct gaggttttcg ccgcgttctt tgatgatgcc gctgcagttt
     1621 cggatttctt tgccgccgct gcgctctgag aggcggcttc ggcgttgcgt gccacttctt
     1681 ccaccattgc ctcaaaacga cgcaatgcct ccggcatgac atcatcttcc gtcatggcac
     1741 cgagaaaatc attcagcgta cctggtctgg agccttcata gacggtaatg gtcccggcat
     1801 gtgaaggcgg aaaaccttca accagcaggg tgacgctgta ctggccatac tcaacatcca
     1861 tgctgtaacg tccggcttca tccggatttt cagaagccac cgtgttcacc agtaccgtgg
     1921 tgctgttacg ctttgccctc agttgaatag tgcagttctg tattggtttt cccgcaccat
     1981 ctttcagcac acctgagatt tttactgctg ccatatccac tccacaaaaa agcccgcctg
     2041 aaccggcggg ctgtcataac actgtgttac ctggctaatc agaatttata accgacaccc
     2101 acgatgaaac cgtcagtgcg ccagtcacca ctgccggaac cttcataagc aaggtcaatg
     2161 gccacggatt cggtcgggtt aaactgcacg ccagcccccc acgccagaga cgtgttgctg
     2221 tggcgaccgt catcacttcc ggtcagcaca tcatgcgttt tccccttgtt gtcagttacg
     2281 cggagataat ccccggagaa ggtcgacaca cggctgtaag ccatacccgc catcgcataa
     2341 gcactgaacc attcattcac gcgtacagat ggccccgcca tcatgctgaa ccagcggtta
     2401 cgcacggaat cttcatgcca gcgggtatcg ctgtaatgcg ttttttgctc atctttggca
     2461 ttggcataac tgaatgacgt caccagcccc agcgtgtccg taaattcata acggtatttc
     2521 acgttaatgc ccttcaggtc atcactgcct ggcatatcag tatgggtctg aagatacccg
     2581 gcgcttagtg tggactgatg ctctgctgcg ctcgctggcg taccagcggc aaccagccag
     2641 actactgcgg acagaataac agcacataat ttacgcataa ttacctctcg cttttctgca
     2701 ataaaaaagg cgtcatttct gacgcccgta ttggggttat aaaattcagc tgatactgat
     2761 gcctgcggtg gctttcttca tcaccacaac cagcaaatcg ctgatacttg ctgtgggata
     2821 ccagtcattt accagccatg ctgacaccga aaactccagc gtcatgtgac cgtgaccggc
     2881 aggcatatca ataacgccac tgtaaatcag cgtattatcc agcgcggtac ggttataaat
     2941 ttcagcaccg tttttccgca ctatcagacg gcatgaggag taaatatcag tatgctctct
     3001 ctcatgttta gcgccgctga atgccaccgc cggaataaca atctgccggt caaacggctg
     3061 atcgtcataa atcctgacgg taatggtccc tgatggccac cgttccggtg cacgggagtc
     3121 ccgggggaaa gctttgccca ctgttttaac gagatcgcct tcaatctggt tcgcggacag
     3181 ttttcccaga acccggcagt tctcgttaat cgtgacgttg ttgagcgtcc cggagttcgc
     3241 attcacgtta ccgctgatat cggcattttt cgccgtcagc cgcccgtccg gtgtcaggga
     3301 aaatgccgga ggattaccgc cgctggtaat ggtgggagcc gtcagatatt tcaggaacac
     3361 ttcattcata aatatctgat cgccctgacc aacaaacatc ggctttgtgt tgccattcgc
     3421 aggattaatc atcgcaatcc tgtctgccgc cagcagcacc tgactctgca tgccgtcagg
     3481 ggtgttttca ataccggcac caatacccgc gatataaagg cgtccgtcct gcatctgctg
     3541 cagcttcaca gcccacatgc tgttcaggtt attatttgta tcaacctgaa ccttctgtat
     3601 ctgctggatc gctgcactct ggtcttccag tttcttattg acggtctgcg tgatttcatt
     3661 actgacatcc gtgatggacg ttctgatttc cgccaggtca ggcgcaagct gaccgttatc
     3721 aatctgcgtc cacaactcct gagccagatg ggttttccct atctctcctt tgaaaaaatc
     3781 cagatagccg gatgcatcat cactcggctg gccaacagcc tccacgaatg ccgatttgcc
     3841 aacggtgttc acactgcgga tgtaaaaata ataatcatgg cccggcttga tattgatact
     3901 ggcagctatc cagtacagcg ccgtgccaag atagcgggct gtggtttcaa cctgcctgat
     3961 atccgcaatc cgcttttccg agaaccagaa ctcaaactgt accgtcggat cataaaccgc
     4021 aagatgcggc gtggcggtta tctgaaaata gcccggcgtc agcttaatcc tcgacggtgc
     4081 tgccggtgcg gcaatccgga acgataccga cgccggatcg ccctgctgcc cccacgcatt
     4141 taccgcccgg actgtcagcc tgtagttccc cagcgccagt tgcctgaagc ggtatgtggt
     4201 ttccgccgtc cgggccgtgc tgaccagcag ctcactgccg tcatccgctg ccacggtcag
     4261 gcgaagcagg aaactcacgc ccttcaccac cttcggcgtg tcccagcgcg ccagcacctg
     4321 atattccccg ctgtctgcgg tgacttcggc ggtcaggtgc tgcaccgctg gcggcgtgac
     4381 accattcacc gtgccgctct ggtcgccgtc aaagtgcgcc ccgttatcca cgatggcctc
     4441 tttttccggt acatgctgca cggcggtgat ggcatacgtg ccgtcgtcgt tctcacggat
     4501 actcacgcag cggaacaggc gctggcgcag cgtcggcagc ttcagccccc acacgctgta
     4561 ttcagcaacg ccgtcaggaa cacggctcac tttcaccttc acgccgtcgg tgacggactg
     4621 gacctccacg ctgaccggat taccct
//
LOCUS       DAYPHN010000237         4629 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000237 DAYPHN010000000
VERSION     DAYPHN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.237"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(53..910)
                     /gene="sitD"
                     /locus_tag="ACG0BW_004535"
     CDS             complement(53..910)
                     /gene="sitD"
                     /locus_tag="ACG0BW_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFK7261401.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(907..1764)
                     /gene="sitC"
                     /locus_tag="ACG0BW_004536"
     CDS             complement(907..1764)
                     /gene="sitC"
                     /locus_tag="ACG0BW_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFK7261402.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1761..2588)
                     /gene="sitB"
                     /locus_tag="ACG0BW_004537"
     CDS             complement(1761..2588)
                     /gene="sitB"
                     /locus_tag="ACG0BW_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFK7261403.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEDVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(2588..3502)
                     /gene="sitA"
                     /locus_tag="ACG0BW_004538"
     CDS             complement(2588..3502)
                     /gene="sitA"
                     /locus_tag="ACG0BW_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFK7261404.1"
                     /translation="MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            4190..>4629
                     /locus_tag="ACG0BW_004539"
     CDS             4190..>4629
                     /locus_tag="ACG0BW_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261405.1"
                     /translation="MIDTMKTLSGKEFRKLFNRYETGFYLSEFEYLKDFDPLFPECVG
                     KELKLGYKRKVDVWCEEDAALYAEFIATKERLKRGRLILKINQVWSRVYPAYIYAIEE
                     RINVRAKGRESFKYETVQEKQCKLSIMLDGLKDLECEIAMISSE"
BASE COUNT         1280 a         1084 c         1125 g         1140 t
ORIGIN      
        1 gttgctggaa gccaggaaaa agtatcgggt gttgctgtcc tcggatgttg tgctaattgg
       61 gtgataacag atcctgtgta tgggagtttc cctttttgcg tgcgttgatg ctggtaacgg
      121 caaacgtcat gatgaacaaa accgcgaata agacgacaat cgtcggagct ggtgcgctgt
      181 cgataaaaaa ggacaaccag acaccagtca ttgacactat taccgatatg ccagtcgcca
      241 gtaggagcgc aatatgaaag cgttgtgtca ggagtaccgc aatggcgcca ggcgcaatga
      301 gcagagaaat ggagagaatg atacctaccg ctttcagcgt cgccacaatg gtcagagaga
      361 ccatgcacag cagcccataa tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt
      421 gataatcaaa gctgaacagc aaaaaatccc gccattttac gctaataaca agtgtaacca
      481 gcccggcaat aatcatcgtc tggattatat cgccgatggt tatcccgagc atgtcgccga
      541 aaagaatatg gtcaagatgt acgtctggtt tgactgcaat atacaagatc agacctgcgg
      601 caaacatgcc agaaaaaaca atccccatca ctgtatcttg cttgattcgg ctgttgtctt
      661 tcaggtatcc cgtcgccacc gcgcagaata ctccggcgac gaatgcccct gtagccaaag
      721 gtaaccccag aatccaggca agcacgatgc caggaaaaac cgcatgactc atcgcgtcac
      781 ccatcagtgc ccaacctttc aggaccagaa agaccgacag cagcgcacag ggtatggaaa
      841 ctaccagtga aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc tccagtaata
      901 atgccatcat gagcgatcct ccagcgcctt atgcgcacgc cgacgatttg ccaaaagccc
      961 atgggtgggc gcaaagacaa atgctaaaag aaacagcagt gtctgcgcca ccacgatgat
     1021 tccacctgtc gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac tggttacact
     1081 gccaatagtt acagcaatca tgagcaggcg gggaaaacga tcggttaaca accatgctgt
     1141 tgcaccggga gtcaccacca gacagatcac caggaaggca ccaaccgttt gcagcgccgc
     1201 aacggtcgaa accgacagca gggtgaagaa gaggagtttt aatctgcccg gatgcaatcc
     1261 aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa
     1321 aagcacgatg atcgagagaa tgccaatgat agtgagttgc aggatatccg ccggatcgat
     1381 tgccagaata ttgcccagga cgatggtctg aatgttcaca gatgtcgggt tcaatgacac
     1441 cataaacagc cccaggccaa aaaaggacga gaagatcaga ccgatgatgg catcttcttt
     1501 taagcgggtg cgttggttaa gaaacagcat gctgcccgca gccagtccgc cggaaaagaa
     1561 ggctcccaga gaaaacggaa gccccagcat atatgccccc gcaacacccg ggacaataga
     1621 gtgcgacaac gcatcgccaa tcagcgacca gccttttagc atcagatagc atgacaggaa
     1681 agcgcagaga ccgccgacca tcgccgagac ccacatcgca ttgagcatat attcatagct
     1741 gaagggttcc agtagcacgt tcatctttct tccctctgca ccgctgacgg tcgatgagcc
     1801 acaaaagggc gttcatcgtc agtaataatg ctttcttccg agccgttaag tgtgacatgg
     1861 cgcagtacgc cgctaaaagc cagctctagg ttctcggctg taaaagtggt gtctgtcggc
     1921 ccgcttgcca ggacggtgcc tttgaccatg accgtataat cgcaaaatgt cgtgacggat
     1981 ccgagattgt gtgttgagac aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg
     2041 ctgatgattt tggcttcggt tttcacatca acgccagtaa aaggttcatc cagcaggatc
     2101 acatcaccct gctgtgcaat cgctctcgcc agaaagacgc gtttcttttg tccaccggaa
     2161 agctcgccga tttgtcgatg gcgaaaattc accatatcga cacgttccag cgcatcggtg
     2221 acaatttgac gatccctctt tttggcgata cgcaacatac ccatatgccc atagcggccc
     2281 atcatcacca catcttccac cagtacagga aatgaccagt caacatcttc tgactgaggg
     2341 acgtaggcaa ccaggttttt ctgtaacgcc tgtcgcgtgg gaatacccag aacagatatc
     2401 tttccgctgg tcagacgcac aaatcccata atcgctttaa acaacgtcga ttttccggaa
     2461 ccgtttaccc caaccagagc ggcaatagat ccacttggta ctgtgaagga tgcatcgcgc
     2521 agtgctgtgt gcccgttacg ccaggtgaca gtaacatcat taacgacaat gcctgcagat
     2581 tgcatcatta tttctccctt ttcccagctt taattccctg taccagcgta ctggtagtaa
     2641 ccttaagaag gtcgatatac gtcggtaccg ggccgttttc tgtgctcagg gaatcgacat
     2701 agagcacacc gccgtagtgt gcgccggttt cacgcgcaac ctgacgcgct ggtttatcgg
     2761 aaatcgtact ctcgctaaag actgccggga tatgattttt cttaactata tcaacaacct
     2821 tacgtacctg ctgcggtgtt ccttgttgat cggcattaat cggccacaga taaagctctt
     2881 ttagccccaa atcgcgtgcg agataagaaa aagccccttc actggtgacc atccatcgct
     2941 gattctcagg gagttccgta atctgcttac gcaggggggc aagggtttgg gtaatcttgg
     3001 ctttataagt atcggcattg cgttggtagg tttgtgcatt tgccggatcg tattttatca
     3061 acgcatcacg aatattatcg acgtaaatca gagcattatc tggcgacatc catgcatggg
     3121 ggttaggttt gccctcatag ggtccttcgg tgatccctac aggcgtcaca cccgaagaga
     3181 caattacttc tggaacccca ttgagatgct ggtaaaagcg ttggaaccac aattccagat
     3241 tcataccatt ggcgagaatc agttgtgccc cctgcgcacg tttaatatcg ccaggggtag
     3301 gctgatactc atgaatttct gcaccaggct tggttatgga tgagacttct gcagcatctc
     3361 ccgccacgtt tttggccata tccgcgatga tggtaaatgt tgtaatgacc ttgaattttt
     3421 cagttgcact tgcctgaaat gcgatcgagc aggtgagtgc gagccccccc aagagcatgg
     3481 ttactttttt tatcgagtgc ataatttagt acctatagtg agcaaaatta gaacaaacat
     3541 agcaacggct atgttttata gcacagccta tacaaaatga aaatgattat cattagcagc
     3601 tttgaggccc aaaacaggga ttaggttgtt tcctgctggc gacgcgttat tgaggaaaaa
     3661 agtaaggcag ctagtaggct ttgaaataac gttttgcggt tggctgatgc aatttcgaga
     3721 gtgcatgtat tgaatgattt ctagccgtta ctgattttcg tattttttgt atgggaggat
     3781 ttgcacctcc ccccaccgag cctccatgac tttactccac tgtctctagg gctgctgtgt
     3841 gccaggagcg gaagcagggg gggccagaga ctccttaaaa gttgatagct cgttttgcag
     3901 ggagacttac acggtaagcg ttattccgaa gagcttaaaa ttgaagcagt caaacgggtt
     3961 gttgagcgtg tttatgcgct gccatctgat taatcgacgg aagcagttcc gtttggtcta
     4021 tgatgcagtt gtccggcagg cgctctgtga cgcaaaagaa cgataggagt agcattgaag
     4081 tgaacgaggt tatgcttgtc ttatattatc ctctatttta aaaacatcag ctggttgtgt
     4141 aagagttttt attgttttat cttgttgact ttgcttttat gaaaacagag tgattgatac
     4201 aatgaaaacc ttaagtggga aagagtttag gaaattattt aatcgttatg agacaggatt
     4261 ctatctaagt gagtttgaat acttaaagga ctttgatcct ttgtttcctg aatgcgtagg
     4321 gaaagaatta aagttgggtt ataaacgaaa agttgatgta tggtgcgaag aggatgctgc
     4381 gctatatgca gaatttatag ctacaaaaga aaggctgaaa agaggccggt taatcctaaa
     4441 aataaaccaa gtgtggtcgc gtgtatatcc tgcttatatt tatgcaattg aagaaagaat
     4501 aaatgtcagg gcaaagggga gagagtcatt taagtatgaa actgtgcaag aaaaacaatg
     4561 taaactttcc ataatgttag atggtctgaa agaccttgaa tgtgaaatag ctatgatttc
     4621 aagtgagtt
//
LOCUS       DAYPHN010000238         4570 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000238 DAYPHN010000000
VERSION     DAYPHN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.238"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..250
                     /locus_tag="ACG0BW_004540"
     CDS             <1..250
                     /locus_tag="ACG0BW_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFK7261406.1"
                     /translation="TNDNVPGLLSLITAHLKDLPDDGRNEDVFKMLRSSAAILHGINN
                     LRNNYSMAHPTETLLNEADARFAINLVRSIMTYVDELL"
     gene            complement(409..1380)
                     /locus_tag="ACG0BW_004541"
                     /pseudo
     CDS             complement(409..1380)
                     /locus_tag="ACG0BW_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420487.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2136..3113
                     /locus_tag="ACG0BW_004542"
     CDS             2136..3113
                     /locus_tag="ACG0BW_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFK7261407.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3398..4138)
                     /locus_tag="ACG0BW_004543"
     CDS             complement(3398..4138)
                     /locus_tag="ACG0BW_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7261408.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4259..4447)
                     /locus_tag="ACG0BW_004544"
     CDS             complement(4259..4447)
                     /locus_tag="ACG0BW_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFK7261409.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPRHDWL
                     NTGSLLQNIPLRIWFYPQ"
BASE COUNT         1128 a         1171 c         1129 g         1142 t
ORIGIN      
        1 tacaaatgat aatgttccag gcttgctttc cctgataact gcacatctca aagacctgcc
       61 tgatgatgga agaaatgaag atgtgtttaa aatgttgaga tcatctgccg caatacttca
      121 tgggatcaat aacctgcgta acaattacag catggcccat cctactgaaa ctttgctgaa
      181 tgaagccgat gcccgttttg ccataaatct ggttcgttcc atcatgactt atgttgatga
      241 gttgctttag ttttaataaa aatatgagac tcacagcgaa cagatagaac ttcaacgaag
      301 aagtgaagta cggccagaat ttcgagttct ggccctccgg aagatgccgg acagacgcat
      361 catcccgtcg tggccgcttc catttttgtg tgtactcaac ctgccggatc aggatggcgg
      421 caggatttgc acccgatgtt ccagccgcct tgcctcttcc tgatcccggt attccctcgc
      481 ctttttcatc atttctgccg ttggctcctg cagtttgtcg agaaactcct tagatcgttt
      541 agccaccgac tgaagggaga cactgtccgt cctgccggta ttgcgattgg tgtaaaccag
      601 gcgggtaagg gggtctggtt aactccttcc cttccataaa gcgtttgaag atccagagat
      661 atatatcaac cagctccagc cctgcacttt ttgtacccga ctggaaaacc agcgttcagc
      721 cggcatgttc gtcacatcca tgaccggtaa accgggaccg tgtacccacg gcatctcacg
      781 tatctggtaa tagaactccc tcagctcccg ctgggtggtg ttgaactgtg actgctggtc
      841 cacaacgatg tcggctttgc gatttggtgc tcccaggcgg gaacagatac cgcgcaatac
      901 gaactgaaat ccaaccatat tgggcatgat ctgcagcttt tgtttaccgg ttttgcagtt
      961 atatcccagt tcgtcaaagt gggtcatcgc ccaggacaac gcatcggtaa tcaattcaat
     1021 gtaacggcgg tcccccaggg tatgaacgcg cgcaagcagc tcactgcaga cctcactgaa
     1081 cagagatgag gaccttccat catgggcttc gaggcgggca caccacgctt tttcagccag
     1141 ttcattatcg aacaggcttg ccagattcag tagcagcggg taacgaaagg gggtccagta
     1201 ggcaaccctg ggcactgcag gattcatccc ctgatcgaag aacgaaataa tggcgtgatc
     1261 ccgtttaacg acctgccaga catcaaaccg tatccggtgc ttcttctgca ggacaagcag
     1321 cgtatcaacc acctcatcaa ggcggtgcat gccaagctct gcggcgtgca gacgctgtac
     1381 ttgtcaacgc cacgatcttt gaccgtctta tatcgacaga gttatccggc atggtgtcaa
     1441 tccgttcaca atttttccat cggagcagcc taccaccggt gataaccaga ctttgcctgt
     1501 agtttgtata cacaatccct ggcattgaaa cacacatatg aagtgtactt cggatatgac
     1561 cgtacgctca ggaagtacgg cagattccgt caaaaattac ctgtaaagac agacatcaga
     1621 aaaacagtgc tcgccgcaga ccggtgaccg ggcgaagccc gcgaaccggc gaggaagcgc
     1681 aatggatctg tagttacagt gagcaggtac gccctgaagc cttcataatc ggtagtgtat
     1741 gctgtttttc tggaatgatt tatccacata tccggaggcc cgatccagta aacagatcca
     1801 tgaatgatca acaaaggatc cattaaagat ccccatatct ctgcaaacct tgtcactcat
     1861 gggccgggac tacgatcaca taagctgtgg catgttactg ataaactgta acatgctaat
     1921 gataagctgt attcagtaat ccatatactg aagtaagtta atgacataaa ctatggtcag
     1981 tatgccagac tcagttgtta aatacaggct gcaggttttt cttcagtcag ttagcggagt
     2041 tctgacacac gattttctgt ttattctttt actgtccaca ggctggaggc tttctggaaa
     2101 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg
     2161 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
     2221 tctttgtacc gacccttaag tcactgaaga acagtaaaaa aaatacactg tcacgcaccg
     2281 atgccacgga agaactgacg cgtctttctc tggcccgtgc agaaggattc gataaggttg
     2341 agatcaccgg cccccggctg gatatggata acgatttcaa gacctgggtg gggatcattc
     2401 attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg
     2461 caaaactgtg tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc
     2521 cttccctgaa gcgcattgcc ggtaccgtga tctcgttttc ccgtaccgat gagaagcaca
     2581 cccgggaata catcacccat ctggtacagt cagcctacta cgatactgaa cgggatattg
     2641 ttcagttaca ggctgatccc cgcctgtttg aactgtacca gtttgacaga aaagtccttc
     2701 tccagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctctacacct
     2761 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc
     2821 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagt
     2881 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaattcttct
     2941 gtattcacta ccggcgtccc cggttaaaag cgccgaatga tgagagtaag gaaaatccgt
     3001 tgccaccttc acctgcggaa aaagtcagtc cggagatggc ggagaaactt gccctgcttg
     3061 aaaaactggg catcacactg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     3121 ctgtagtcag tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag
     3181 tggtctgctc actgctgttt atattcagtt tcctgcagtg ctgcctgtgg ctgagctgcc
     3241 atctgcctgt cccttacgtg agtcaccccg taacctgatg ctgaggcatt gctcccttca
     3301 taaaacatgg tttactcact acagcttatc tacctgctcc agcttatgtt atgtctgttc
     3361 tgctgaccac agcttatata aggaagcgcg tatgccgtca cttcagggga ggcagtgtac
     3421 gcaggatctc cgcagcatcc cgaccgtcac ctgtgaaagg cactgccagc gtggcagcca
     3481 tatccagtgc aaacactctg gtataaacct ccatcgaacg tggatccctg tgaccagcca
     3541 gtgcctggat gactttccgg ggctggcggt gatagagcat gtgcatgata tagctgtgcc
     3601 ggaaggtgtg tggtgtgacc ggaatcgaaa agtgtacccc gtcagcttcg gcccgtctga
     3661 cagcctgctt cagccagttg cgcatggttt cgtcggtcac ggcccataat ggttcacgac
     3721 gacggggccg ggtggtgatc atccagcttt ccatctgcct gacatagctt atatctgtca
     3781 gcggaaccag gcgcacttca tcttttggcg ggcgtccgcg tcgcgcacgc actttttcgg
     3841 acaggatccg cacaaacggt cttactccat ccaggtcaaa tgattccggc gtcagcatcc
     3901 gggcttcgcc aatacgcatt ccggtattcc agagggtggc gaacagcata tggtgacgct
     3961 gatccggcat atagaagaga agggcactca cttccggggc cagcaggtag gccggtgtgg
     4021 cccccgtgga agtggccatt cttctcaggg agagcgctgt tgcaaaatcc acccccggcg
     4081 caatgggtaa cagggagacg cgttctggtg aattctgcag gggaatgaca ttgttcatga
     4141 gttatttttc acgtagcaca cgcactgttt atggatacag gtattctgac ctgaacgccc
     4201 ggatccttcc acgacaataa atcgcagcgc gcggatcctt ttgtttgctt atcacgcatt
     4261 attgcgggta aaaccaaata cgtaaaggta tgttttgcag cagggatccc gtattaagcc
     4321 agtcgtggcg cgggctgagg gctggtttgc cgtttttggg agaattgggg taatgccagg
     4381 aacaggcaga tacggagaaa aatcctgcag ataaccttct acgacaactg aaggtgtctg
     4441 acgccatatt cttagctact tctcagtttt aaggcggctt agtcacaaca taaggttatg
     4501 cataataagc atattttatt atgttatgtt atggtcttgt tcatgcatga actgttatct
     4561 attcagtcac
//
LOCUS       DAYPHN010000239         4553 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000239 DAYPHN010000000
VERSION     DAYPHN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.239"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..262)
                     /locus_tag="ACG0BW_004545"
     CDS             complement(<1..262)
                     /locus_tag="ACG0BW_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFK7261410.1"
                     /translation="MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERA
                     VKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKRAADRLKS"
     gene            581..1249
                     /locus_tag="ACG0BW_004546"
     CDS             581..1249
                     /locus_tag="ACG0BW_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFK7261411.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            1255..1608
                     /locus_tag="ACG0BW_004547"
     CDS             1255..1608
                     /locus_tag="ACG0BW_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261412.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(1661..2428)
                     /locus_tag="ACG0BW_004548"
     CDS             complement(1661..2428)
                     /locus_tag="ACG0BW_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261413.1"
                     /translation="MDDLSVYARSLDKAKYYVYCLYDTEDKMKRPFYIGKGKSTRCLD
                     HIKYPDDSPKSMRIKELLANKKLGIDILRHGMDETTAKLVEATCIDLMGVGELTNKVR
                     GSGSMMGRISLDAYHHLVLQEETEIAPEHAGLAFLLNSTYKSGMSALALYEATRGVWA
                     KVPRDENLKYAYATYGGLIMEVYQIECWVKAGSQQYFTRDIALGPDTKRYEFVGRIAD
                     EHIRKLYVGKLIKKPPSYGSPFVKVGVVRTEHSVSAA"
     gene            2711..3451
                     /locus_tag="ACG0BW_004549"
     CDS             2711..3451
                     /locus_tag="ACG0BW_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261414.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            3495..>4553
                     /locus_tag="ACG0BW_004550"
     CDS             3495..>4553
                     /locus_tag="ACG0BW_004550"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFK7261415.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGASKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRA"
BASE COUNT         1226 a         1046 c         1048 g         1233 t
ORIGIN      
        1 tgcttttcag acgatcagca gctcgtttca gcttgtcgtt cggtgccgga ggattgctta
       61 tagcgtccat aaccagattc caggattctt cattcagaac tagtctacgg tgttgctcta
      121 taactttcac ggcacgttca gatgcactgc taaccataaa ctgagtaatg ctctggttag
      181 acattgcggc agcttcctcg atgatgcttt tatcgtcatc ggttaatctc aaatcgatgc
      241 gctgcttttt tagtgctgac atgtattcct cctgatggcc gtgatgtagg aaggccatca
      301 atctcctctg ttcaaactga gttttcgact caaaaattac tttaaaaggc tatagcccat
      361 tacgtgtgca ttatcaaatt gtgtacggca tatcaccgta cttgtaatat agaggtcatt
      421 gttacttttt tcaacagaca aatacaagct atgtttgcca attgttaatc acaacctacc
      481 atttaacctt tacaccgcag ccgtaggcat ttaggctata tcacatataa gaaaacaagt
      541 tgtttcagac gataattata tacgcaaagg gaactctcca atgaccaaga tctttgtggt
      601 tggcggcaca aagggcgggc ctggcaaatc caccgttgcc cagcaaattg ccgtttgcct
      661 gaaagtcaaa aagaagaaga aggtttatat taccgatata gatattcagc gcacgacaac
      721 gagctggtgt gaagaccgtc gacagaacga agaccttgag ctgattcctt ttgcatacgt
      781 tcaggatgac atcattaagc acctaaaatc gcttcagggt agagctgagt ttgtagtggt
      841 agatgctggt ggcttcgact ccgaaattca gcgacaagcg atgctgatgg ctgacgttat
      901 cattatcccg ctgcgtccta agcgtcgtga tttgaaatct ttacgtgaca tcgatcctat
      961 tatcgacaat gttcgcaatg taaacgataa agtgaaggtc cgcgcggtca tgaaccagtg
     1021 cccggctttg ccatcacaag tgtctcgcat tctggcggct aaagagattg ttgagacgtt
     1081 tggaatcgag tctgcgccag tcaatctata taaccgcaac gtctatgatg atgcggaaga
     1141 gtctggtcgt tctatctttg aaatgaccgg tagcgagcgc gacaaaaagg cggaagccga
     1201 gtttgaagaa tttgtagatt atctgttgag tctggaggaa gaagaataat gtccatgaaa
     1261 atgggtgacc tagcaaagcg caaagagcct gatgcaccgg ctaagaacac aactcctttg
     1321 cgccaaccag tcagaccaca gggacgcccg actcgtggca aagagaaaat taaaagccgc
     1381 acaatgtcac tggaggacga atacttcgaa ctgctggaga tgatgaagtt catccctcgc
     1441 ttcgagaagt tcactcgttc tgacgtgatt cgagcagcca ttttccatct ggcagagaag
     1501 tcaccgcagg aaatcgagga catcgtgaaa ttgaatgagg cgatcaccgc tgccgatgtc
     1561 acgatgcgta ccgatgaaat caaacgagag ttgatgaaga aaggttaaaa atcatgcatt
     1621 ggcgcgtaca ttgcgccaat gcataactac agtgtcagct ttatgccgcg ctaacactat
     1681 gttcagtcct aaccacccca accttcacaa aagggctacc gtagcttggt ggcttcttga
     1741 ttaatttgcc tacatacagt tttctaatat gctcatcggc tattctgcca acaaattcat
     1801 aacgttttgt gtcggggcca agagctatat cccttgtaaa gtactgctga gaaccggctt
     1861 tgacccagca ttcaatctga taaacttcca ttatcagccc accatatgta gcgtaggcat
     1921 atttgagatt ctcgtctctt ggaacctttg cccatacgcc acgcgtagct tcatataatg
     1981 ccagagcgga cattcctgac ttgtaggtgc tgtttagcag gaaggcaaga ccggcgtgct
     2041 caggagcaat ttcagtttcc tcttgcagca ctaagtgatg gtaggcgtct agtgatattc
     2101 tgcccatcat ggaaccactt cctcgtacct tattcgtaag ctctccgacc cccataaggt
     2161 cgatgcatgt cgcctcaaca agtttggctg tggtttcatc cataccatga cgaagtatat
     2221 ctatgcctaa ttttttatta gccaaaagtt ctttgatccg catggatttg ggagagtcat
     2281 cgggatactt gatgtgatcg agacaacgag tcgatttgcc ttttcctatg taaaacggtc
     2341 ttttcatctt gtcttctgta tcatagagac agtaaacata gtatttagcc ttatccaatg
     2401 atctcgcgta taccgataaa tcgtccatta ttgcatctct ttgctactga ctaatgcgtt
     2461 ctattttaca cataagtttt taatcaattt aacaggctca aagtgtagag cttctgggcg
     2521 tctaagacga caagttatgc ctgcttccgt atatataaat aataagtaac ttattaaata
     2581 tatacggaag caggtctttt aaaagacacc accagaacaa ctcccttccg tttccacttc
     2641 caaaaactgc caccagtcgc tatcatccgc tcattgtgat aagtaagtaa ctacctacca
     2701 ggtgagccac atgagccaaa tctttttcga taccatcgac aacgaccagt acgacttcat
     2761 gacagagtgg aataccgctg ttatggacaa gtgggtcgct gaaaacattg gtttgtcgcg
     2821 ctgtaaagac gaggctgaac tcttcgagac gaagtggttt gattaccgcg acatgcatcc
     2881 tctcatggcc acctgtcttt ttacggaggc atacaaacgt cagtactcaa atatcatgct
     2941 gacgcacggt cgcgaacact ttgaaacagc tccgttcacc accgggttaa aacgtctgcc
     3001 ttatcaggag ttgtcgactg ccaataaaac gtcgctatgg aaagcacgcc agtttgcgga
     3061 tcgctattgc tgctcatacg actactttat ctccaccgtt ctttccgcag ctgcacgacg
     3121 gctgtgggac aagctgccgc gcccacagca tttatggcag ccagaactga ttgagatatt
     3181 tgaagagaaa ttagccagac gcgcaacaac ccgtctggat gactctctgg ttagctttaa
     3241 gcatatggga gacatgcagt tcaacccgat tcaggaaagc tattttgagt ggattctgga
     3301 gcgtttgcgc accatccctc gcagcaagcg catacgcgca attttctccg ctatctggct
     3361 aatggaaatc gttccagagc gccttatttc cgcccacttt ccagaagaac tggaagaagc
     3421 acggcggttt attgatcccc tatctaatta actaatacta gaaaacaatt tgtttaaaaa
     3481 acaaaggaaa gcacatgacc gaactttgcc atacaggacg cgggctgtcc gaagagtttg
     3541 atgaagattt ccagaacaga ctgacggcat atttttgtcg tgatcacgag tttcttactc
     3601 gtgcgggaga tctggttgtg cctagccagt ttgccaatgc ggccaatgcc atattggtta
     3661 atatggtttc gggctattac cgtatgtaca agagcgcgcc ctcttcatct gcaattctgg
     3721 atatgcttaa gcgtgcgaaa cgcgataaga ctatccgtga ggaactattc gccgatgttg
     3781 ttgctgcgtt taagcgcatc cttgcagaaa aattgtccga tacctcgtac atggttgacc
     3841 aggtatcaac cttcgcaaaa agtgtagcgt ttgatgatgc tctgattaag gctgctgaac
     3901 tgaaagagaa aggcgacttt cagggggcga tggcaatcat ggccaaggtt cagcagattg
     3961 gatcgaacga agcgaccgga atctatgact actacacctc cgcaagtgag cgattgaaag
     4021 cgcgtgaata tgaggcttca gaggagtatg taccaaacag tattacaact ggactccctc
     4081 ttctcgatag gttgctgtac caaaaaggct gggcaaagcg cgaaatggtg ctcttcatgg
     4141 ggttcgctaa atccggtaaa tcgaccgcaa tgggtgagtt ttccataaac gcaacacttg
     4201 ctggctacaa tgttctgtat ctctcgctgg aggttcacac caccatttta tccgaccgtt
     4261 ttgatgcaag attgtcggag acggaaatgt ccaagttggt ggaacggcgc gatgaagttc
     4321 atcgtaagtt ggcagagttg ggagcctcta aggggattgg tagtttgtgg gttgttgagc
     4381 gtccgtcagg aagtatgtca ccggcagatc tggaccgtat gcttaacagt atgaaagcca
     4441 atggcatggt gcctgacatg gttgttgtcg actacgcaga tttgatgcgt gccagttatg
     4501 accttcgtga tgatcgcgcc aacattcgta gtatctacac cgatttacgt gct
//
LOCUS       DAYPHN010000240         4451 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000240 DAYPHN010000000
VERSION     DAYPHN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.240"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..441)
                     /locus_tag="ACG0BW_004551"
     CDS             complement(<1..441)
                     /locus_tag="ACG0BW_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFK7261416.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELL"
     gene            complement(556..631)
                     /locus_tag="ACG0BW_004552"
     tRNA            complement(556..631)
                     /locus_tag="ACG0BW_004552"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Thr,seq:ggt)
     gene            complement(638..712)
                     /locus_tag="ACG0BW_004553"
     tRNA            complement(638..712)
                     /locus_tag="ACG0BW_004553"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(677..679),aa:Gly,seq:tcc)
     gene            complement(829..913)
                     /locus_tag="ACG0BW_004554"
     tRNA            complement(829..913)
                     /locus_tag="ACG0BW_004554"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Tyr,seq:gta)
     gene            complement(922..997)
                     /locus_tag="ACG0BW_004555"
     tRNA            complement(922..997)
                     /locus_tag="ACG0BW_004555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Thr,seq:tgt)
     gene            1359..2309
                     /gene="coaA"
                     /locus_tag="ACG0BW_004556"
     CDS             1359..2309
                     /gene="coaA"
                     /locus_tag="ACG0BW_004556"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFK7261417.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2338..3303)
                     /gene="birA"
                     /locus_tag="ACG0BW_004557"
     CDS             complement(2338..3303)
                     /gene="birA"
                     /locus_tag="ACG0BW_004557"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFK7261418.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3300..4328)
                     /gene="murB"
                     /locus_tag="ACG0BW_004558"
     CDS             complement(3300..4328)
                     /gene="murB"
                     /locus_tag="ACG0BW_004558"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFK7261419.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQHVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHYVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1140 a         1115 c         1011 g         1185 t
ORIGIN      
        1 cagcagctct tcgtcatcaa ccatgtcgca tttgttcagg aacacgatga tatacggaac
       61 gcctacctga cgacccagca ggatgtgctc acgagtctgc ggcatcgggc cgtcagtcgc
      121 agcaactacc aggatcgcgc cgtccatctg agcagcaccg gtgatcatgt ttttaacata
      181 gtcggcgtgc cccgggcagt ctacgtgtgc gtagtgacgg gtcggggtgt cgtattcaac
      241 gtgagaagtg ttgatggtga taccacgagc tttttcttcc ggcgcgttat cgatctggtc
      301 gaatgcgcga gcagcaccgc cgtaggtttt agccagtacg gtagtgattg cagcggtcag
      361 cgttgtttta ccatggtcaa cgtggccgat agtaccgacg ttaacgtgcg gttttgtacg
      421 ttcaaacttt tctttagaca tcgattgtcc ctctaagaca cggataaatc ggtgatatca
      481 ccacatcaac caggcaacat gcccgacttg ttgaatgcaa taaacagaag gaaaaaacag
      541 gaaggagaaa agaagtggtg ctgataggca gattcgaact gccgacctca cccttaccaa
      601 gggtgcgctc taccaactga gctatatcag cacatcttgg agcgggcagc gggaatcgaa
      661 cccgcatcat cagcttggaa ggctgaggta atagccatta tacgatgccc gcatcctgga
      721 actcggctac ctgattttca ttctgcactg aatatcgaga gaagctctct ttattcgagc
      781 cggtaagcga acttatcgtc tcgggctacg ccatcgcgtg gccgaaattg gtggtggggg
      841 aaggattcga accttcgaag tctgtgacgg cagatttaca gtctgctccc tttggccgct
      901 cgggaacccc accggacttg atggtgccga ctaccggaat cgaactggtg acctactgat
      961 tacaagtcag ttgctctacc tactgagcta agtcggcatc aagtagcgcg cattctatgg
     1021 agacatgcga gttcatgcaa ctaaaaaatt gcataatttg ttttattggt cacattttat
     1081 gcggcacgat gaagaaacag ccgaacggag ggcagattag cacacttttt caacattatt
     1141 gtgctcaaca atgcgctcct gctaaaccat aattcttttt atcagatgga atatctgtca
     1201 cattgctttt caacgatagc ttcctgggag agattttttc ttattattcc tccccatctg
     1261 gtgttaccct cctgcccatt aacccattca acagaactgt gacgcgccat ggcaaatatc
     1321 gctttgccga tagagctatg accgccagaa acatgcttat gagtataaaa gagcaaacgt
     1381 taatgacgcc ttacctacag tttgaccgca accagtgggc agctctgcgt gattccgtac
     1441 ctatgacgtt atcggaagat gagatcgccc gtctcaaagg tattaatgaa gatctctcgt
     1501 tagaagaagt tgccgagatc tatttacctc tgtcacgttt gctgaacttc tatataagct
     1561 cgaatctgcg ccgtcaggca gttctggaac agtttcttgg taccaacggg caacgcattc
     1621 cttacattat cagtattgct ggtagtgtcg cggtggggaa aagtacaacc gcccgtgtat
     1681 tgcaggcgct attaagccgt tggccggaac atcgtcgtgt tgaactgatc acgactgatg
     1741 gcttccttca ccctaatcag gtactgaaag aacgtggtct gatgaagaag aaaggcttcc
     1801 cggaatcgta tgatatgcat cgcctggtga agtttgtttc cgatctcaaa tccggcgtgc
     1861 caaacgttac agcacctgtt tactcgcatc ttatttatga tgtgatcccg gatggagata
     1921 aaacggttgt tcagcctgat attttaattc ttgaagggtt aaatgtctta cagagcggaa
     1981 tggattatcc acacgatcca catcatgtat ttgtttctga ttttgtcgat ttttcgatat
     2041 atgttgatgc accggaagac ttacttcaga cgtggtatat caaccgtttc ctgaaattcc
     2101 gcgaaggggc ttttacagac ccggattcct attttcataa ctacgcgaaa ttaactaaag
     2161 aagaagcgat taatactgcc atgacgttgt ggaaagagat caactggctg aacttaaagc
     2221 aaaatattct acctactcgc gagcgcgcca gtttaatcct gacgaaaagt gctaatcatg
     2281 cggtagaaga ggtcagacta cgcaaataat tttcagggga gctaatgctc ccctatctta
     2341 tttttccgca ctacgcaggg atatttcacc gcccatccag ggttttatta ttccatcctg
     2401 ctcaagcaat aaagcgccct gtttgtctat tccgcgtgaa atgccaaata tttctttatc
     2461 accaatgata agtttcactg ggcgattaat aaaattatcc agcttttccc agcgcgaaag
     2521 ataaggtgcc aatccttctt gctcgaagag ttccagcgcc gcgcgtaatt cacgtatgag
     2581 catggccgcc aacgtattac gatcgagatt gatccccgct tcctgcagcg tgatccaccc
     2641 ctgattaacg acactctctt caacacgacg cattgccatg ttgatcccgg ctccaatgac
     2701 tatttgcgcc gcatcgccgg ttttaccagt cagctccaca agaatgcctg ccagcttacg
     2761 atcctgcaga tagaggtcat taggccattt aacacgaact ttatctgcac ccagcttgcg
     2821 taatacttcc gccatcacga taccgataac cagacttaaa ccaatcgccg ccgccgggcc
     2881 ttgttccaga cgccagaaca tcgacaaata taagtttgcg ccaaaaggcg aaaaccattt
     2941 ccgaccccgg cgaccacggc cagcctgctg gtattctgca acgcaggcat cgcccgattt
     3001 aagctctccg atacgatcaa gaaggtactg gttcgtggag tcaataacgg gcagcacagt
     3061 tacgctaccg ccatccagct gacccaatat ctgttcagca ttaagtaact ggataggctc
     3121 aggcaggctg tatcctttac ccggaacggt aaagacatca acgccccagt cacgcagtgt
     3181 ctgaatgtgt ttattaatag ccgcccggct cattcctaac gtttcaccca actgctcacc
     3241 agagtgaaat tcgccattcg ctaacagcgc gattaatttc agcggaacgg tattatcctt
     3301 catgaaatcg tctccactgc gctaacttcg cctgatgcac caataaagcg aacttcgggc
     3361 tcaagccaga cattaaattt ttcacccact ttttgtctta cataatgtgc cagttgcacc
     3421 acatcttcgc tttttgcatt gtcttcatta atgagaacta acgcctgttg acggtgcacc
     3481 gcagccccac ccatttgcat cccttttagc tggcactgat cgataagcca acctgctgcc
     3541 agttttactg aaccatctgc ctggggataa tttggtgctg ttggaaattg tgccagtaat
     3601 gctttagccg tttcggcaga tacaacaggg tttttgaaga aactaccggc attgccattc
     3661 acttttggat cagggagttt ggtggtgcgc atatgacaca ccgcattaaa tacttgctgt
     3721 ggcgttactg ttgtaggatc cagacgagtt aagtcaccat acgttagtac aggttgccac
     3781 tcttttggca gacgcagacc tacggctaca atggcgaagc ggtcctggta ttcatgttta
     3841 aaaatactgt cgcgatagcc aaaacggcac tctttggcag ttaagcgcac ctgcttgcct
     3901 gtcgccagtt caacacagtc aacataagcg caaactcgct gtaattctac gccataagca
     3961 ccaatattct ggataggtga tgagccgaca caaccaggaa ttaatgccag attttccaga
     4021 ccaggcatac cttcctgcaa agtgtatttt accaggcgat gccagttttc tccggctcct
     4081 acatgtaaat accacgcatc aggttcatca tgaatttcga tacctttgat ccggttgatg
     4141 atcaccgtgc cgcgatagtc ttccagaaaa agtacattac ttccttcacc cagaataaga
     4201 acgtgttgtc cttctgcggt tgcatgctgc caggcattga gtagttgttg ttcgtcttcg
     4261 gcacatacaa tgtgctgagc attatgatca atgccaaatg tgttccaggg ttttaaggag
     4321 tggttcatag ctgctttcct gatgcaaaaa cgaggctagt ttaccgtatc tgcgggggga
     4381 tggcttgtag atatgatgac aggaagagtt tgtagaaacg caaaaaggcc cagtcttccg
     4441 actgagcctt c
//
LOCUS       DAYPHN010000241         4418 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000241 DAYPHN010000000
VERSION     DAYPHN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.241"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..455
                     /locus_tag="ACG0BW_004559"
     CDS             <1..455
                     /locus_tag="ACG0BW_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFK7261420.1"
                     /translation="AADNLTFKGKLIIPACTVQNAEVNWGDIEIQKLVQSGGNQKDFT
                     VQMTCPYHLGTMKVTITATNTYNNAILVQNTSNTSSDGLLVYLYNSNAGNIGTAITLG
                     TPFTPGKITGNNADRTISLHAKLGYKGNMQSLKAGDFSATATLVASYS"
     gene            530..1033
                     /gene="papF"
                     /locus_tag="ACG0BW_004560"
     CDS             530..1033
                     /gene="papF"
                     /locus_tag="ACG0BW_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial tip protein PapF"
                     /protein_id="HFK7261421.1"
                     /translation="MIRLSLFISLLLTSVAVLADVQINIRGNVYIPPCTINNGQNIVV
                     DFGNINPEHVDNSRGEVTKTISISCPYKSGSLWIKVTGNTMGGGQNNVLATNITHFGI
                     ALYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFTSVPFRNGSGILNGGDFRTTA
                     SMSMIYN"
     gene            1077..1693
                     /locus_tag="ACG0BW_004561"
                     /pseudo
     CDS             1077..1693
                     /locus_tag="ACG0BW_004561"
                     /inference="COORDINATES: protein motif:HMM:NF015583.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PapG carbohydrate binding domain-containing
                     protein"
     gene            1697..2083
                     /locus_tag="ACG0BW_004562"
     CDS             1697..2083
                     /locus_tag="ACG0BW_004562"
                     /inference="COORDINATES: protein motif:HMM:NF015584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PapG chaperone-binding domain-containing
                     protein"
                     /protein_id="HFK7261422.1"
                     /translation="MLSFNNTGSCRPSAQSLEINHGNLSIDSAHGNYASQAVTIYCDV
                     PVTVKISLFSNTQPAYNNQGVAVGLGNGWDSIIYLDGVKRNEETLRWNTAGSRTVTVG
                     SKLYGEAGKITSGALSGSMTMIMHLP"
     gene            2222..3387
                     /locus_tag="ACG0BW_004563"
                     /pseudo
     CDS             2222..3387
                     /locus_tag="ACG0BW_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3554..4105
                     /locus_tag="ACG0BW_004564"
     CDS             3554..4105
                     /locus_tag="ACG0BW_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFK7261423.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGAYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT         1310 a          839 c         1037 g         1232 t
ORIGIN      
        1 atgcagctga taatctgacc tttaaaggaa aactgattat tcctgcctgt actgtacaaa
       61 atgctgaagt taactggggt gatattgaaa ttcagaagct ggtacagagt ggcggaaatc
      121 agaaagactt tactgtccag atgacctgtc cttatcattt gggaacaatg aaggttacga
      181 taacggcaac aaacacttat aacaatgcta ttttagttca gaatacatca aacacatctt
      241 ctgatgggtt acttgtttat ctttataaca gtaatgcagg aaatattggg actgcgataa
      301 ctttagggac tccatttacg cccggaaaaa tcacaggtaa taatgcagat agaactatat
      361 cacttcatgc caaacttgga tataaaggga atatgcagag tctgaaggct ggggattttt
      421 ctgcgacagc aacgctggtt gcatcatatt cgtaatagta tcaactaaaa tacgccgatt
      481 ttatatctca taaaataaaa tgttttctgt gccgctctcc ggagggggga tgattcgttt
      541 atcattattt atatcgttgc ttctgacatc ggtcgctgta ctggctgatg tgcagattaa
      601 catcagggga aatgtttata tccccccatg caccattaat aacgggcaga atattgttgt
      661 tgattttggg aatattaatc ctgagcacgt ggacaactca cgtggtgaag tcacaaaaac
      721 cataagcata tcctgcccgt ataagagtgg ctctctctgg ataaaagtta cgggaaatac
      781 tatgggagga ggtcagaata atgtactggc aacaaatata actcattttg gtatagcgct
      841 ttatcaggga aaagggatgt caacacctct tacattaggt aatggttcag gaaatggtta
      901 cagagtgaca gcaggtctgg acacagcacg ttcaacgttc acctttactt cagtgccctt
      961 tcgtaatggc agcgggatac tgaatggcgg ggatttccgg accacggcca gtatgagcat
     1021 gatttataac tgagtcatac ctaaatgaat aactgtaatt acggaagtga tttctgatga
     1081 aaaaatggtt ccctgctttt ttatttttat ccctgtcagg ctgtaatgat gctttagctg
     1141 gatggcacaa tgtaatgttt tatgctttta atgactattc tggttatgat tctggtaata
     1201 tgacaatttt tgacagggga caattcacta ttccatggaa aacaggagca gcatccgcaa
     1261 tctatagctc atgtcagact ccagaattcg tgagcggtgt ttactttcag gaatatgtag
     1321 catgggtgtt agttccgaga agtgcccaac ccactgatgg atatactgtt tttttgatgt
     1381 ctacagtaaa tatggctgga atcaggaaaa tacaggtgat tatgggtatt attatttact
     1441 caacggatat gaatgggata cttggacaag cgatgggggg cgggtgtgtg ctccggtagg
     1501 aaatacaaaa caattgagta acactttcaa tgaattaagg tttagtttac tgttgcctgc
     1561 agatctaccc aaagggcgct atgaggttcc aattaaatat atcaggggga tacagcatca
     1621 ttattataat ggctggcgtg agcattataa aatgccctac agtcaggtta agcagttacc
     1681 tgctacaaat actctaatgc tgtcatttaa taatactgga agttgtcgcc cttccgctca
     1741 atctcttgaa ataaaccacg gtaatctttc tattgatagt gcccatggta attatgcaag
     1801 ccaagctgtt accatttatt gtgacgtccc ggttactgtt aaaatctcac tattcagcaa
     1861 tacacaacca gcatataata atcagggggt tgcggttggt ttgggcaatg gctgggattc
     1921 aataatatat cttgatggtg tcaagcgaaa tgaggaaaca ttgcgctgga atactgcagg
     1981 atccaggaca gtgacagttg gaagcaagtt gtatggtgaa gcaggcaaga taacttctgg
     2041 tgctttgtct ggttcaatga cgatgatcat gcatcttccc tgaatacaaa accatgttta
     2101 tggtgagaca ggagagaaat ttcacctggc gtttctatgc aacgaatgat gtatagtgga
     2161 tcggtaattc aggacacctc gaaatcttgt taatgttaaa acagtgaaaa tgaggtgatg
     2221 cataatcaaa actcgtcgga ctaaacgtac ctttttcccg gagttcaagc ttgaagctat
     2281 cgaacaggtg gttaaatacc agcgtgatgt cagagaagtc gcgctggcac ttgagctcaa
     2341 tcctgaccat ttgcgtaaat ggatacggtt gtataagcag gaatttcagg gtattgagtc
     2401 cgctggtaat gctattatcc ctgaacaacg cgaaattcag cagcttaaag cgcagataaa
     2461 gcgcgttgag atggaaaaag aaatactaaa gcaggctgcc gtgctgatga gcgaaatccc
     2521 cgggaagctg tcgcgctaat cacacggctg aaaacaaagt ggccagtgtg ggttatttgt
     2581 catttattcg gtattaaccg tagcgtttat tacgcgcagg tgacgcgtcc tgttaatgtg
     2641 caaagaattg acttacgaag tcgggtgagg gctttccatg ctctcagtcg tggcgcagcc
     2701 ggtagccgtg caatcagtca gatgttgcgc cagagtagcg ttgatgcagg ccggtggctg
     2761 gcacgacgac tgatgcggga atgcgggctg acaagtcgac agatggttaa acatcactac
     2821 cgggtcaacg aagacaaaag tccgccattg ccaaatttac tgaaccggca atttcacccc
     2881 gccgcaccag accgcgtctg gtgcggcgac atcagtttta ttcgcctgca tgacaaatgg
     2941 tgttagctcg cgctcgttgt tgatttatat tcacggcgga ttaccggttc agccctctca
     3001 ttaaccgctg atgctgagtt ggtgtgtagg gctttgcgta atgcactgga aacacgatca
     3061 cgtgatggcc gtctgctgtt tcattcagat caaggtgttc agtataaaag caataaatac
     3121 agaaagttac tctggcgtta tggggtaatg caaagtatga gttgcagagg gaatggcctg
     3181 gataattcgc cgatggaaag agtctttcgc agccttaaaa atgaatggct tccgaaaggt
     3241 ggttatggtg attttatcca tgcggtacgt gatataaacc agtggattaa tggttattac
     3301 aacgtttatc gacctcacac gaacaacgat ggtttaccgc cttgcttaca tgaagaaaag
     3361 tggaaacagg ttattccggt gtcctgattt tgtgaaccac tacaataatc ctttttcaca
     3421 gacttgtcag cagccagcat ttatgttctt ttacctgcgg gaatttatcc gtctgcctgt
     3481 cgggactact ctgtcataca gaaatcaggt aggaataaat tgtagtggaa agtcgatgtt
     3541 taccggatga ctgatgcgcg cttgtacaca gacagtgtgt ttcagtaata tggagaataa
     3601 tgaaatgaat aacacagaca cattagaaaa aataatcaga caccaaaaaa acaaagaccc
     3661 cgcatatccc tttcgggaac atttgttgat gcagctctgt attcgcacaa ataaaagaat
     3721 gcaggataat atatctgaat ttctgggggc gtatggaata aatcactcag catatatggt
     3781 tctcaccaca ttattcgcag cggagaacca ttgtctgtca ccttcagaga taagccagaa
     3841 acttcagttt accagaacta atattacccg cattacagat tttttagaaa aagccggata
     3901 tgtaaaaagg acggatagca gggaggatcg ccgtgctaaa aaaatcagtc tgacatctga
     3961 aggtatgttt tttattcaga ggctcactct tgcacaaagc atgtatctga aagaaatctg
     4021 ggattatctg acccatgatg aacaggaact gtttgaagtc attaataaaa aattactggc
     4081 acatttttct gatgccagct cataaagtgc gaaatatctg aggatgctgg atagcttcag
     4141 gcaagagaat aatgattctt gcagatgtgt ttttccggat acaaaaacaa aagtaaaaaa
     4201 ttgcagcgcc aggccacctt tcaaagcagg gaggcctgta ccgcgtcgaa aatttcagcc
     4261 agttaatgta attgtctgaa ccaggcactt cgcccgggca ggagaaggag cgtggttgcg
     4321 gtcatcctgt atactgaagt cgttatgaag gaataataca aataattgcc cgttttctct
     4381 gtacgataga ggcaggtgtg tatgattgca tcactatg
//
LOCUS       DAYPHN010000242         4361 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000242 DAYPHN010000000
VERSION     DAYPHN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.242"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..494
                     /locus_tag="ACG0BW_004565"
     CDS             <1..494
                     /locus_tag="ACG0BW_004565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7261424.1"
                     /translation="GEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEG
                     RPKVLGYYTLSGSCFEKESLPSRSQQKKVPYRNVPSVTLGRLALDKSLQGQGFGSMLV
                     THAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSLFYPTKSIEKL
                     FEE"
     gene            complement(662..913)
                     /locus_tag="ACG0BW_004566"
     CDS             complement(662..913)
                     /locus_tag="ACG0BW_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261425.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVEVVNETSNKIRQKDASAVHRELYDKWVRH"
     gene            complement(915..1607)
                     /locus_tag="ACG0BW_004567"
     CDS             complement(915..1607)
                     /locus_tag="ACG0BW_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261426.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGTSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMV"
     gene            complement(1621..1944)
                     /locus_tag="ACG0BW_004568"
     CDS             complement(1621..1944)
                     /locus_tag="ACG0BW_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261427.1"
                     /translation="MSNEMAGVTTEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KTYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIVSKITSFIITALLLGLAAYGV
                     KSGLQ"
     gene            complement(2147..>4361)
                     /locus_tag="ACG0BW_004569"
     CDS             complement(2147..>4361)
                     /locus_tag="ACG0BW_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261428.1"
                     /translation="SKSDISGNKVYANDEVIVRSQNALRMIAGDYGVIWRNDGANTYL
                     LMTDKGDQYGGWNGLRPFAVNNATGEVTINTPLNSPKGVKGNSDTATKLQTARKISGV
                     PFDGSTDITLTAAHVAAFARRATGSYADADGGVPWNAESGAYNVTRTGDSYILANFYT
                     GVGSCRTLQIKAHYKNGGLFYRSSRDGSGFESGWEQVYTTGFRPQPADINAPTAAAGW
                     LNSGNGTAFTTAQFITWLNNQGAFSNKYWIARCSWTYANNNYIDDTGCGRIDLSGSVI
                     EVFSNKSTSHYTIRVTTTTTSGHGGVNNAEFIYVYNGSDYSPGWRRSYNTRNKPTASD
                     VGALSLSGGALTGGLTAAGEIISKSANGLRIAYGNYGFFIRNDGSSTYFMLTDSGNSL
                     GTYNSLRPLIINNANGAVTIANGLNVTGGVNGSLNGNASTATKLQTARNINGVKFDGS
                     GDININTLVSRGRVTALSGSTQGTAGIQMYEAYNNSYPTMYGNVLHMKGASAAGEGEL
                     LIGWSGTSGAHAPVFIRSRRDNTGAAWSAWAQVYTAKDSIPGVNTTGNQNTTGNAATA
                     TKLQTARKIAGVAFDGSADITLTAANLNAYTKTEVTNLLSSYVKSSALPSMTVRTSSV
                     SGGDMGMSLSAFISHLKSNGAFSKSYWIGFGDAMGFNAGSINNITGFGAVELAESIIE
                     VFNLPNGDYTIRLTTSHKADYGGVTNAILVYHYRSNRSPSGQWLKFAGTVGATSN"
BASE COUNT         1151 a         1081 c          888 g         1241 t
ORIGIN      
        1 ctggagagaa agattatgat ttgaatggtt ttgattgcgg tgaagaatca ctaaatgcct
       61 ttttaaccaa ccacttaaaa aggcagcatg agggaaaaat tcttcgcgct tatgtgcttt
      121 gcacgaaaga aggcagacca aaagtgttag ggtattacac tttgtcaggt agctgctttg
      181 aaaaggagtc cttaccttca agaagccagc aaaagaaggt accttatcgg aatgttccaa
      241 gtgttacttt gggtaggctg gctttggaca agtcccttca aggtcaagga tttggctcaa
      301 tgcttgtaac acatgcaatg cgcgttgtgt acaatgcatc tcttgctgta ggcattcatg
      361 gacttttcgt tgaggcgtta aatgacaaag ccaaggcgtt ttataaaagt ttagacttta
      421 tccagctggt tggtaacaac gagcgttctt tgttctatcc tacaaaatct atcgaaaaat
      481 tgttcgaaga ataatgtact cccctcaagt gaggggaact tcgcatcacc aaccgcaaac
      541 tctctctccc atcgtattgt gaaaaaggat ttggcgttcg gtttcttcgg tcatcacatc
      601 attattgctg atatagatag gcccagctcc atcgcagaac aatacgctgg cggtttgtgg
      661 tttaatgacg cacccactta tcatacaact cacgatgaac ggcagaagca tctttctgac
      721 gtattttatt gctcgtctca ttcaccactt ccactgtgct ttgaagtcgt tttctgtctt
      781 cctgttttgc cttctcttcc attgctcgtc gcgccgcatt cccgcccatc gtgtaagcgc
      841 cgacaagaac gaaaagaacg gcagccagcg taatcaaagc aacttttagc tttgtcatca
      901 ggctgcctag catattagac catccctttc tggtgttttc ttacctgcga ccaggcaatg
      961 aatcctgcca caacaatagt ggcaataccg aagatgatgc gtactgtatc cccgctagag
     1021 atatgacctt gtgctttatc catagcagcg gaaacctgcg gcataacatc ggccagctgc
     1081 gccagaccaa tacctgctgt aacagttgcg cctgcggttt ctttagttac aggaacagcc
     1141 ttcacggttt tcaccggctt aacaacgcca gctcgacgca gaccttcctc aataacttct
     1201 gccgcatacc aggtgttcag cgtttttagc gggcctcggc cattctcgtg gcgaatgatt
     1261 gcctcaacca aaggtcgaag gatgtcgtaa tcatgcagat cgatgatcat gtctgcggtt
     1321 acaccaacgg ctttagacac ctcattaatg taggcgtcag tattgttttc attcggcggt
     1381 gcccaacgtt caataacttc acgaatggta tcgatacttg tgccgtcttt tgcgcgacgt
     1441 ttatcgtggt aggtaattag agtcaccgcc agcgcacgaa tcccccaaac agggtcttta
     1501 aacgtgcaaa agcgcggttc gtctggattc gcaaccagtc cttgccacgg tgatccttta
     1561 tcaagattac cggggttatt attacgaatg cctctcgggg tcttcatcct tgatctcctg
     1621 ttattgcagt ccacttttta cgccatacgc ggctaacccc aaaagcagtg cggtaataat
     1681 gaacgacgtt attttagaaa caatgccgcc aaagaaccca cttgagatgg aatctaaccg
     1741 gttaaggagt ttgtccagat tggagtgttg aatactatgt tgcgccgggg tcatatcacc
     1801 aaagtaggtt ttcagctgat cattgacctc ctggccaatt tcttcacgta gctctttacc
     1861 taatttgcca acaacctccc gagcaacgat cgcggcaata cgctcaactt gctctgttgt
     1921 aacgcccgcc atctcgttcg acatgttttc ctccatgaaa agtcaaatcg ggatggcgga
     1981 tttatatcac acttctcctt tcaattatag gtatatactt acctatctat gcatatgccg
     2041 tttaacggct gttcaaattc gtatctagta actcactaat atatccctat gattgcggct
     2101 gtgagatcgc attttaaacc tcacagccat ataaataatg tctaaatcag ttacttgttg
     2161 ccccaacagt acctgcaaat ttcagccatt gacctgacgg acttctatta ctacggtagt
     2221 gataaacgag gattgcgttt gtaactccac cgtagtcagc tttatgtgac gttgttaaac
     2281 gaattgtata atcaccgtta ggtagattaa aaacttcgat tatagattct gctaattcta
     2341 cagcaccaaa tcccgttata ttgttgatac tgccagcatt gaatcccata gcatcgccaa
     2401 agccaatcca ataacttttg ctaaacgccc catttgattt taggtggcta atgaatgcgg
     2461 acaagctcat gcccatatcc ccacctgaaa cagaggaagt tcgcacagtc atgcttggaa
     2521 gagcactgct cttcacatag gaacttagaa ggtttgttac ctctgttttc gtgtaagcat
     2581 taaggttagc cgcagtcaaa gttatatcgg cagagccatc aaacgccaca ccagcaattt
     2641 tccttgctgt ctgcaatttt gtggcggttg cggcattacc agtagtgttc tgattaccag
     2701 ttgtattcac accagggatt gaatccttag cggtatatac ctgcgcccac gctgaccatg
     2761 ccgcacctgt gttatctctt cgtgagcgaa tgaaaactgg cgcatgtgca ccgctcgtac
     2821 cactccagcc aataagcaac tctccctcac cagcagcact cgcacctttc atgtgcaata
     2881 cgttgccata catggtcggg tagctattgt tgtacgcctc atacatttga atgccagcag
     2941 tgccttgagt agagccgctt aacgccgtaa ctcggccacg agataccaat gtattaatat
     3001 tgatatcgcc tgaaccatca aacttaacac cattgatgtt tctcgctgtt tgcaacttcg
     3061 tagctgttga agcattgcca ttcaagctac catttacgcc accagtaaca ttgagtccat
     3121 tagcgatcgt aacagcacca ttggcattgt taatgataag cggccttaaa ctattatagg
     3181 ttcccaggct gttacccgaa tcggtcaaca tgaagtatgt gcttgaacca tcgtttcgga
     3241 taaagaatcc gtagtttccg taggcaatgc gcagaccatt cgccgatttt gaaataatct
     3301 cgccagcagc agttaatccg ccagttaacg ctcctccaga aagcgataat gctcctacat
     3361 cagaagctgt tggtttgttt ctagtattat aagaacgacg ccagcctggc gagtaatcac
     3421 tgccattgta cacataaata aactccgcat tattaacacc accatggccc gatgttgttg
     3481 tcgttgtaac acgaatagtg tagtggcttg ttgacttatt actaaacacc tcaatcacgg
     3541 aaccagatag atcgatcctg ccacatccag tgtcgtctat ataattattg ttggcgtaag
     3601 tccatgagca ccgtgcaatc caatatttgt ttgaaaatgc accctgatta tttagccacg
     3661 ttataaactg agcagtcgta aatgctgttc cgttaccact atttaaccat ccggcggcag
     3721 cggttggtgc gtttatatca gccggctgag gtctaaatcc agtcgtgtaa acctgctccc
     3781 aaccactttc aaaaccactt ccatcgcgag atgaacgata aaacaaccca ccatttttat
     3841 agtgagcctt aatttgaagc gtacggcaac tacctacacc agtataaaag ttggcaagaa
     3901 tataactgtc gcccgttcta gttacattat aagcaccaga ttcagcattc cacggcacgc
     3961 cgccatcagc atcagcatat gaccctgttg ctcttcttgc gaaagcggca acatgcgcgg
     4021 cggttaaagt gatatcagta gagccatcaa atggaacacc ggatattttt cttgcagtct
     4081 gaagtttcgt agctgtatca gagttcccct taaccccttt tggagaatta agtggtgtat
     4141 tgatcgtcac ttcacctgtt gcgttattta ctgcaaatgg tcgaagaccg ttccagccac
     4201 cgtactggtc gcctttatca gtcatgagca agtaagtatt tgctccatca tttcgccata
     4261 tgacaccgta atcgccagca atcatcctta acgcattctg actacggaca ataacttcat
     4321 cattagcgta aaccttatta ccgctaatgt ctgacttact c
//
LOCUS       DAYPHN010000243         4294 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000243 DAYPHN010000000
VERSION     DAYPHN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.243"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..2252
                     /locus_tag="ACG0BW_004570"
     CDS             <1..2252
                     /locus_tag="ACG0BW_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFK7261429.1"
                     /translation="KLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKT
                     TSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFGR
                     VNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINAL
                     AGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVM
                     AAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTYE
                     NKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNRS
                     DALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPSG
                     QQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDKEGG
                     FNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQAG
                     FNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSLPEVIGNGWEGIS
                     DEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTHFGAG
                     DITELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLPQ
                     IPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGRT
                     WIFGGDIRF"
     gene            2321..3496
                     /gene="senB"
                     /locus_tag="ACG0BW_004571"
     CDS             2321..3496
                     /gene="senB"
                     /locus_tag="ACG0BW_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFK7261430.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4019..>4294)
                     /locus_tag="ACG0BW_004572"
     CDS             complement(4019..>4294)
                     /locus_tag="ACG0BW_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFK7261431.1"
                     /translation="FFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEAVAKVCYAQMV
                     KQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNARDISLLRYMPA"
BASE COUNT         1195 a         1029 c         1016 g         1054 t
ORIGIN      
        1 taaaactggc tatcggctca ggcatattat tgctcagctg cggtgcttac tcacaatcca
       61 tcagtgaaaa aactaattcc gacaaaaaag gagcggcaga attcagtccg ctcagcgttt
      121 ctgtcgggaa gacgaccagt gagcaggaag ctctcgagaa aacaggcgcg accagttccc
      181 ggacaacgga caaaaacctg caatcacttg acgcaacagt gcgtagtatg cctggtactt
      241 atactcaaat agatcctggt cagggagcaa tcagtgtgaa tattcgaggc atgagcggat
      301 ttggtcgtgt aaacactatg gtcgatggta ttacccagag tttttacgga acctctacct
      361 ccggaacaac gacgcatggt tcaactaaca atatggctgg cgtacttata gatcctaact
      421 tactggtagc agttgatgtt acacgcggtg acagcagtgg ctctgaaggg atcaacgccc
      481 ttgccggtag tgcaaatatg cgtactattg gcgttgacga tgtaatattt aacggtaata
      541 catatggcct tcgttcacgt ttctctgtcg gtagtaatgg gctgggacgc agcggaatga
      601 tcgcccttgg tggaaaaagc gacgctttta cggatacggg aagcattggc gttatggctg
      661 ctgtgagcgg cagttctgtg tactctaatt tctcaaatgg ttctggaatt aacagcaaag
      721 agtttggtta tgataaatat atgaagcaga accccaaatc ccaactgtat aaaatggata
      781 tcagaccaga cgaatttaac agcttcgaac tttccgctcg aacctatgaa aataaattta
      841 cacgtcgtga tataaccagt gacgactatt acattaaata tcattacacc cctttttctg
      901 aattaattga ctttaacgta acggccagta ccagtcgcgg taatcaaaag tatcgtgatg
      961 gctcgctgta tactttctac aaaacctcag cgcaaaatcg ttctgacgcg ctggatatca
     1021 acaataccag ccggttcact gtcgcggaca atgacctgga gtttatgctg ggcagcaaac
     1081 tgatgcgtac ccgctatgac cggaccattc actcagcggc gggcgacccg aaagcgaatc
     1141 aggaatcgat cgagaacaat ccgttcgcac cctccggcca gcaggatatt tcagcgctgt
     1201 ataccgggct gaaggttacg cgcggcatct gggaggcaga tttcaatctc aactacacac
     1261 gtaacaggat cacagggtac aagcccgcct gcgattcacg cgttatctgc gtgccacagg
     1321 gtagctacga tattgacgat aaagagggtg gcttcaaccc ttcagttcag ctttctgctc
     1381 aggtaacacc atggcttcag ccgttcattg gctacagcaa atccatgcgc gccccgaaca
     1441 tccaggagat gttcttctct aattcaggag gcgcatccat gaacccattc ctgaagcctg
     1501 aacgtgcaga aacctggcag gcgggtttta acattgatac cagagattta ctggtcgaac
     1561 aggatgccct gcgctttaag gctctggcgt accgcagcag gatccagaac tacatctaca
     1621 gcgagtctta tctggtttgt tctggaggtc gtaaatgcag tctgcctgag gtgattggca
     1681 atggctggga gggcattagc gatgaataca gcgacaatat gtacatctac gttaactcgg
     1741 caagcgacgt tatcgcaaag ggcttcgaac tggagatgga ttatgatgca ggttttgctt
     1801 ttggccgact ctctttcagc cagcagcaaa cagaccagcc aacctccatc gccagcaccc
     1861 actttggcgc aggggatata accgaactgc ccagaaaata catgacgctg gatactggtg
     1921 ttcgcttctt cgataacgcg ttgaccctgg gcactatcat aaaatacaca ggcaaggctc
     1981 gtcgcctgtc gcctgatttt gagcaggacg aacataccgg cgcaataatc aaacaggatt
     2041 tgccgcagat cccaacgatt atcgatctct atggtactta cgagtacaac cgcaacctga
     2101 cactgaaact ttcggtacaa aacctgatga acagagatta ttcggaggcg ctgaataagc
     2161 tcaacatgat gccaggtctt ggtgacgaga cccacccagc caattccgcg cgtggcagaa
     2221 catggatatt tggcggggac attcgtttct gatgacaatt tccgctaggt tcagacaata
     2281 cgttttctct cttatgtcaa ttttattgca ggaacgaaaa atgaatattt tcactttatc
     2341 caaagcaccg ctatacctgt taatttcact atttttaccc acgatggcca tggctatcga
     2401 tccacctgaa cgcgaacttt cgcgatttgc cctgaaaacg aattaccttc agtcccctga
     2461 tgaaggcgtc tatgaactgg cgtttgataa tgccagtaaa aaggtgtttg cagcagtcac
     2521 cgatcgtgta aatcgtgaag ccaataaagg ctatctgtat tcgtttaatt cagattcgct
     2581 gaaagtcgaa aataaataca cgatgccata ccgggcattt tcgctggcga taaatcagga
     2641 taaacatcag ctctatatcg gacacaccca gtcagcgtcc ctgcgtatca gtatgtttga
     2701 caccccaacc ggcaaactgg taagaaccag cgacaggtta agttttaaag cggcaaacgc
     2761 tgcagattcg cgttttgagc attttcgcca tatggtttac agccaggatt ccgataccct
     2821 gtttgtgagt tatagcaata tgctgaaaac ggccgagggc atgaagcctc tgcataagct
     2881 gttaatgctc gacgggacga cgcttgcctt aaaaggcgag gttaaggatg cttacaaagg
     2941 tacagcgtat ggtctgacga tggatgaaaa aacacagaaa atctacgttg gcggaagaga
     3001 ttacatcaac gaaattgatg cgaaaaatca gacgctgctg cgtaccatcc cgttgaaaga
     3061 tccgagacca caaatcacaa gtgtgcagaa tctggcggtg gactccgctt ctgaccgtgc
     3121 ctttgtggtg gtattcgacc atgacgatcg ttccggtaca aaagatggac tctatatttt
     3181 tgacttacgc gacggtaaac agcttggcta tgtgcacaca ggagccggag ctaacgcggt
     3241 gaaatacaat ccgaaatata acgaactgta tgtcaccaac ttcactagcg gcaccatcag
     3301 cgtagtggat gccaccaaat acagcatcac ccgtgaattt aacatgccgg tctacccaaa
     3361 ccagatggtg ttgtcggacg atatggatac cctttacatt ggcatcaaag aaggctttaa
     3421 ccgcgattgg gatcctgatg tgtttgtgga aggagctaaa gaacgtattc tgagcattga
     3481 tttgaaaaag tcgtgatcgg ggggaaaaaa catgggataa aaactccgtt attccactaa
     3541 actggccgga ctaggaaaag caaacatctc tgtcctgccg gtacgcctgt tacgccgctc
     3601 catgaggctg aggaatctcc agaaaacgag acaggcacag cattgtacga tcaactgatt
     3661 tgtgccaata attaaatgaa atcacagcta tgcctgttca tggggttcta ggccggaacc
     3721 ccagattttt ccgattgttt gttttctaac tgattattca gtagttttag agggctgacg
     3781 ctcagtggaa cgaaaactca cgttaagagg ttttgactat gataaaagtc aaagtagggg
     3841 tgagtttgga tatggaaatt attgtactat aaggagagca tgaaaaaccc tggggcagtc
     3901 actgaatgag ggaccagcag cggtaaaata accagcataa tttctgcatg gtcgattttt
     3961 ataccgtgac aggctgtttt ttatacttgc accgattccc gggcacactt agcctgtgtc
     4021 aggccggcat atacctcagc agactgatat cccgcgcgtt tttcttcagc cctgatacat
     4081 tcagccccgc aatggcccgg cggtatacca tccggccgcc gcacagcacg cattcgaacg
     4141 ggtcacgact caggaactgt ttcaccattt gtgcatagca cactttcgcc actgcttccg
     4201 gtttatccat ccccagagca cggtacacct gcggcagctt ctctccacac acccggttgg
     4261 caaggaaccc gaagtacctc accatcttaa aaaa
//
LOCUS       DAYPHN010000244         4198 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000244 DAYPHN010000000
VERSION     DAYPHN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.244"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(216..731)
                     /gene="pgpA"
                     /locus_tag="ACG0BW_004573"
     CDS             complement(216..731)
                     /gene="pgpA"
                     /locus_tag="ACG0BW_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFK7261432.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(709..1686)
                     /gene="thiL"
                     /locus_tag="ACG0BW_004574"
     CDS             complement(709..1686)
                     /gene="thiL"
                     /locus_tag="ACG0BW_004574"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFK7261433.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRAGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADVKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1764..2183)
                     /gene="nusB"
                     /locus_tag="ACG0BW_004575"
     CDS             complement(1764..2183)
                     /gene="nusB"
                     /locus_tag="ACG0BW_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFK7261434.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2203..2673)
                     /gene="ribE"
                     /locus_tag="ACG0BW_004576"
     CDS             complement(2203..2673)
                     /gene="ribE"
                     /locus_tag="ACG0BW_004576"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFK7261435.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2762..3865)
                     /gene="ribD"
                     /locus_tag="ACG0BW_004577"
     CDS             complement(2762..3865)
                     /gene="ribD"
                     /locus_tag="ACG0BW_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFK7261436.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHH
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQHNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     ILPGLEKLADALQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3869..>4198)
                     /gene="nrdR"
                     /locus_tag="ACG0BW_004578"
     CDS             complement(3869..>4198)
                     /gene="nrdR"
                     /locus_tag="ACG0BW_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFK7261437.1"
                     /translation="FEVAELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDV
                     EMAINHIKSQLRATGEREVPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGE
                     EIARLED"
BASE COUNT          993 a         1187 c         1040 g          978 t
ORIGIN      
        1 tcagaatata tgagtaatta ttatgagttc gtcgactgac gatatttcag tgtcattgaa
       61 ttatgagtga caaagttaca ccttgaaata tctccgcata atatattcgg tgatgtcgga
      121 ttttacttta acagagtgcc tcaaaattgg ggcactgttc atttataaat caaaccgggt
      181 ggtctggaat cgcctacacc tcccggtcaa tggcattaaa gaatacccag cggccagtga
      241 tgaccgataa agtacaggat gcctgcggaa attaccccgg cgacaatatc gtcgatcatg
      301 atccccatgc cgccatgtac attgcggtca aaccagcgga tcggccacgg tttccacata
      361 tccaggatac ggaaaatcac aaacccggcg gcgacccact gccagtcatt ggtcggcagc
      421 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
      481 acgcccatat cttttgcggt ctgatgacaa agatagacgc cgatacagat ccccagcatc
      541 accaccagcg agtagagctg ccagggcaaa aaggtcatca gataccagaa cggaatcgct
      601 gccagcgagc ccatcgtccc aggaacgatc gggcttaatc cacttccgaa tccgacagca
      661 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtggc
      721 aaaatggtca tatcctttcc agtctaacgt gacaggttcg ccgtcacgaa taaaacaaag
      781 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccca gatgtccgag
      841 agccacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
      901 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
      961 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
     1021 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
     1081 tgcctgtccc tgtaaaatac gcggagatgg acggagatga cgtttgatca agtagtccgc
     1141 atctttaaca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
     1201 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagcgc gcgttaaggc
     1261 tcgtcccatc ggaacaaagc cgtggatacc cagcgtcatt gataatggcc cacgcgtggt
     1321 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
     1381 ggactcaagc cacgcttcgt cgacgtccgg taaggttaat gccagcgtca gccaggctgg
     1441 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat acgccagatc
     1501 agcaggatcg atatcaggga ggaagtggtt gcccgccacc agcgtatcag tgctgatcgc
     1561 cagggtctgt ttctcgggga tattgagaag tgcgcaatcg tcgccaatgc ccagttcgac
     1621 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
     1681 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcgcg gaacggtctt
     1741 ccgtgaatct accggcctgg atatcacttt ttgttagggc gaatcacagg tgctgcttta
     1801 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
     1861 atcgcttcgt taatggccac tttgtatggc acatcgctac gtttagacag ctcatacagc
     1921 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtat
     1981 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
     2041 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
     2101 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
     2161 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
     2221 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
     2281 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
     2341 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
     2401 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
     2461 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
     2521 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
     2581 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
     2641 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
     2701 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
     2761 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
     2821 taaattggag ggcgtcagct aatttctcaa gccctggtag tatgcataat ccacgggcgt
     2881 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgca
     2941 gcaatgcgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatt tgctgtttac
     3001 ccagttgcat cattagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
     3061 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
     3121 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
     3181 gtaccggctg gcggagattg tgttgcgggt agagcccctg agtttgttca tccagttcag
     3241 accaacgcac cgttaaggcc ggatcatccg ccagcaccgt ggcactgctg gttaaaatgg
     3301 catgactttg tgcgcgcagt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
     3361 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaattgaa
     3421 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
     3481 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacgatat agccctcgcc
     3541 cagcgacctg cgggttagga tcttgcatcg aggcaaccac gcgcgccaca cctgcggcaa
     3601 ttaatgcatc acagcaaggc ggcgtacggc cgtgatggct gcaaggttcg agtgtcacat
     3661 acgcagtcgc acctttggct ttttctccag ccatacgcaa cgcatgtact tcggcgtgtg
     3721 gttcaccagc acggtgatgg taaccttcac cgacaatttc gccatctttg acaatgacgc
     3781 acccgacatt cgggttggga tgcgtggtaa aacgtcctcg ttgcgccagc tttagcgccc
     3841 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
     3901 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
     3961 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggc acttcgcgct
     4021 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcgacg tcatcggaac
     4081 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
     4141 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaa
//
LOCUS       DAYPHN010000245         4159 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000245 DAYPHN010000000
VERSION     DAYPHN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.245"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            64..1220
                     /locus_tag="ACG0BW_004579"
     CDS             join(64..313,313..1220)
                     /locus_tag="ACG0BW_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFK7261438.1"
                     /translation="MSRKTQRYSKKFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNCRFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            1374..3371
                     /locus_tag="ACG0BW_004580"
     CDS             1374..3371
                     /locus_tag="ACG0BW_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFK7261439.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            3434..3772
                     /locus_tag="ACG0BW_004581"
                     /pseudo
     CDS             3434..3772
                     /locus_tag="ACG0BW_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
     gene            complement(3782..>4159)
                     /locus_tag="ACG0BW_004582"
     CDS             complement(3782..>4159)
                     /locus_tag="ACG0BW_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFK7261440.1"
                     /translation="TMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQ
                     SMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYN
                     GGLPPNESENRYWKNSNAVASFC"
BASE COUNT         1013 a         1002 c         1051 g         1093 t
ORIGIN      
        1 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
       61 ataatgagca gaaaaaccca acgttactct aaaaagttca aagccgaagc tgtcagaacg
      121 gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc
      181 actttaggac aatgggttac cgccgccaga aaagggctcg gtactcctgg ttcccgcacg
      241 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
      301 gagcgagata tattaaaaaa agcaactgta gattttgcac aggagtcgct gaaaaatacg
      361 cgttaatcga acaatggcga caacaatttc ccattgaagc gatgtgtcag gtatttggtg
      421 tatccaggag cggttattac aactgggtac agcatgaacc ctcagacaga aaacaaagtg
      481 atgagcggct aaaactggag attaaggtgg cacatatccg cactcgcgaa acatatggaa
      541 cccggcggct ccagacggag ctggcagaga atggcatcat cgttggtcgt gaccgactgg
      601 cacgtcttcg taaggagcta aggctacgct gtaagcagaa acgcaagttc agagcgacta
      661 cgaactcgaa ccacaatctg ccagttgcgc caaatctgct gaaccagacg ttcgctccta
      721 cagcaccaaa tcaggtctgg gtggcggacc tgacgtatgt tgccacacag gagggatggt
      781 tgtacctcgc tggcatcaaa gatgtttata cgtgcgaaat tgtcggctac gccatgggag
      841 agcgcatgac aaaagagctg acaggtaaag ccctgtttat ggcgctcagg agccagcgcc
      901 cacctgccgg gctaatccac cactctgatc gaggttcaca gtactgcgca tacgattacc
      961 gggtcataca ggagcagttt ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg
     1021 acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa tgagagcctg agccactatc
     1081 gttttaataa ccgggatgaa gccatctcag taatacggga atacattgag attttctaca
     1141 atcgtcagcg tcgtcactct cgtctgggga atatctcccc ggcagccttc agggaaaaat
     1201 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
     1261 tcaacattcc accatgcatt ccgattaacg ccgcatagcc agttgaactt tgctactttg
     1321 tgagaggtag taccttctat ccagtgcgaa tttaattaat ggaataaatg attatgagtg
     1381 aaaatgatac aatcccaaag aagtctacaa gtcagattaa caaagcggta ttctttacat
     1441 ctgctttgct aattttcctt cttgtcgcct ttgccgccgt attcccggat gtcgccgaca
     1501 aaaattttaa actacttcag caacaaatct tcacgaatgc cagctggttc tacatccttg
     1561 ctgtggccct gattttactg agtgtcacgt tccttggact ctcacgctac ggtgatatca
     1621 agctgggccc ggaccatgcg cagcctgatt tcagctacca ctcctggttt gcgatgcttt
     1681 tttcggcagg gatggggatc ggcctgatgt tctttggcgt tgccgaacct gtaatgcatt
     1741 atctttcgcc acccgttggc actccagaaa ccgttgcggc agccaaggaa gcaatgcgtc
     1801 tgaccttttt ccactgggga ctgcacgcat gggcaattta tgccattgtg gcgctgattc
     1861 tggcgttctt cagttaccgt cacggtctgc ctttaactct gcgctccgca ctctatccca
     1921 ttattggcga tcgcatatac ggacctgtag gacatgcggt tgatattttc gctgttatag
     1981 gcacggtctt tggcgttgcg acatcactgg gttacggtgt tttgcaggtg aatgccggtt
     2041 tgaaccatct tttcggggtg cccatcaatg aaacggtgca ggtaattctg atcgtggtca
     2101 tcacggggtt agcgacgatt tcagtggtgt ccggtctgga taagggaata cgtatcctgt
     2161 ctgaactcaa tctgggtctg gctttgttgc tcctggcgct ggtcctgtgt ctgggaccaa
     2221 ccgtgcttct gctgaagtca tttgtggaaa atacgggcgg ttatctttcg gaactggtga
     2281 gtaaaacgtt caacctttac gcgtatgagc ccaagtcgag caactggctg gggggctgga
     2341 cattactgta ctggggatgg tggctttcat ggtcgccgtt tgtggggatg ttcatcgcac
     2401 gggtctcccg cgggcgaacc attcgcgagt ttgtcaccgg cgtgctgttt gttcccgcgg
     2461 gttttacgct aatgtggatg acggtgtttg gtaacagcgc gatctatctc attatgaacc
     2521 agggggccac agacctcgcc aatactgttc agcaggatgt gtcgctggcc ctgtttaatt
     2581 tcctggagca tttcccgttc tcttctgtgc tgtcattcat tgcaatggcg atggtcatcg
     2641 tcttctttgt aacgtctgct gattcggggg caatggttgt ggatactctg gcatcaggtg
     2701 gagtggcaaa cacacccgtc tggcagcgaa tattctgggc ctcgctcatg ggcattgttg
     2761 caattgcgct tctccttgcc ggagggctaa gtgcgctgca aacggtgaca atagcgagtg
     2821 cattgccctt ctcagtgatc ttactaatat ccatatacgg acttttaaaa gctttgcgcc
     2881 gggatttgac caagcgtgaa agcctgagca tggcgacaat tgctcctacg gctgcacgta
     2941 acccaattcc ttggcagaga aggttacgca atatcgcgta tctgccgaag cgatctcttg
     3001 tgaaacgttt tatggacgac gttatccagc ccgccatgac gctggttcag gaggaactga
     3061 acaagcaggg gacgataagc cacattagtg atgcagtcga cgatcgtatt cgtcttgaag
     3121 tcgatttggg caacgagctg aattttatat atgaagtgag gcttcgcggg tatatctcac
     3181 cgaccttcgc gctcgccgca atggataatg atgagcagca gactgaacaa catcgatatt
     3241 atcgcgctga ggtttatctc aaagaaggcg gtcaaaatta tgatgtgatg ggctggaacc
     3301 aggaacagct gattaatgac atactggacc agtacgaaaa acacctgcac ttcctgcacc
     3361 tggttcgtta atagcaacat gccgtcctgg gggcggcaat tattatctcg gccgcaatat
     3421 gagggaatgc agaatgattt cacgctggaa atggatgctg aagcagacaa ttaaaaaact
     3481 atggttcagg gcaacgttat tcgcaattgt cgcgataata acggcccttt tatcaattct
     3541 ttttaaatca atgatacctg agtcggtttc cgtgaaggtt ggtgcggaag cagtcgataa
     3601 cattctgaac atactggcat cgagtatgct ggcagtgacc acattttcgc tgagtatcat
     3661 ggtcacagcc tacggttcag ccactactaa tgtgactccc agagctacgc gtttagttgt
     3721 tgaagacgtc accacacaaa atgtactggc caccttcatc ggttcttttc tcttgaagtg
     3781 gtcaacaaaa actggccacc gcgttagagt ttttccagta tcgattttcc gattcgtttg
     3841 ggggtaaccc accgttatat tcgtgcggtc ttagtgcgct gtaatatcca acgatatagt
     3901 ccgttatggc gtgagctgcc tcgctgaagc ttacgtaacc caccaccggc atccattcgt
     3961 tcttcagact cctgaagaag cgttccattg ggctgttatc ccagcagttt ccgcgccggc
     4021 tcatactctg tctgatctgg tatcgccaca ataactgccg gaactgcctg ctcgtataat
     4081 gactgccctg atcgctgtgg aacatcaccc cgccgggctt accacgggtt tcccatgcca
     4141 tttccagcgc tttcatggt
//
LOCUS       DAYPHN010000246         4057 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000246 DAYPHN010000000
VERSION     DAYPHN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.246"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1001
                     /locus_tag="ACG0BW_004583"
     CDS             <1..1001
                     /locus_tag="ACG0BW_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261441.1"
                     /translation="THEYKIDHQQLKNHIRQLEEWALLEKFYDSNAYYSKRTKQLEKK
                     IADAGFIAGKVNADNFNVKEFRADIASMLCEQPPMRTHQISSSGYLSADKVPDLYKTN
                     LKDAQRDLKTTPVDIDGVSLKSKTREKIAKKTEEENAKKTAEIIAKQNAKTIEEKNSE
                     KNKKESNPEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQDLHN
                     VARNGSPRYAGLLRQIASKTAKLQDIDQLLFWRTFSGGVFHRRISVNSEPEKTDVWRF
                     SSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFTYSCYAAPLDANAKK"
     gene            complement(1022..1603)
                     /gene="acpH"
                     /locus_tag="ACG0BW_004584"
     CDS             complement(1022..1603)
                     /gene="acpH"
                     /locus_tag="ACG0BW_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFK7261442.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            1822..2892
                     /gene="queA"
                     /locus_tag="ACG0BW_004585"
     CDS             1822..2892
                     /gene="queA"
                     /locus_tag="ACG0BW_004585"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFK7261443.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            2948..>4057
                     /gene="tgt"
                     /locus_tag="ACG0BW_004586"
     CDS             2948..>4057
                     /gene="tgt"
                     /locus_tag="ACG0BW_004586"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFK7261444.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVP"
BASE COUNT         1077 a          949 c         1039 g          992 t
ORIGIN      
        1 gtacacatga atataaaata gatcatcaac agttgaaaaa tcacataagg caacttgagg
       61 agtgggccct tcttgaaaag ttttatgata gcaacgctta ttattctaaa cgtacaaaac
      121 aattagagaa gaaaattgcc gatgcggggt ttatagcagg gaaggttaat gccgataact
      181 ttaatgtaaa agagtttaga gccgatatag cctcgatgct ttgtgaacag ccccccatgc
      241 gaacacacca gatttcttca tccggatact tatctgccga taaagtgccc gatctctata
      301 agacaaattt gaaagacgcg cagagagatc tgaaaacaac accggttgac atcgatggtg
      361 ttagtctaaa atcaaaaact cgggaaaaaa ttgcgaaaaa aactgaggaa gaaaatgcga
      421 aaaaaacagc ggaaataatt gcgaaacaaa atgcgaagac cattgaggag aagaatagtg
      481 aaaaaaacaa gaaagaatca aatcctgagg ataattttgt cttaaaattc tcaccggaag
      541 aaatcaatga gatgctcctg gattacagcg atgaagaaca aaaaaaatta agaccttttt
      601 tcactagtga tcacatctat tgtttgtttg atgcttttag tcaggatttg cataatgttg
      661 ctcgtaatgg cagtccacgg tatgcaggtt tactacgtca aatagcatcc aagactgcga
      721 aattacaaga tattgatcaa ctattgttct ggcgcacgtt ttcaggaggg gtcttccatc
      781 gacgtatctc tgtgaacagc gagccagaaa agacagatgt atggcgattc agtagccaat
      841 ttgattttcc acatataaat gaaaacggag agttatccag aacggtactc atgttcgagg
      901 gggttgctga aagcaacttt aaagatatcg ccaatcattc cagcgaatgt tttacttaca
      961 gttgttatgc cgccccgctg gatgcgaatg ctaaaaaata gtgacgccgg agtttgaagt
     1021 ttcagagcgc cttgcgtgac gcctgcgcca tcatccgcgg ataaaactgc cagaagcggg
     1081 tttcgagagc atcgtaatgg gcgtctaaat cgtaccagga gtcacgcagg gcatccagac
     1141 gtgggcgacg gcttgccatg ccgtttaaca cgttctggat gaaatccata tcgcgatagc
     1201 gcaccagcca ctgctctgac cataagtagt tgttcagatt gataaagcgt ggcggtgagt
     1261 ccggcaaaat cgtcatcact tgctcgcggg cataacaaac aaattcctgt agtggaaagt
     1321 ccggcgacag ctgcgaccag tggcgggaaa gaaagtgatc ccacatgaca tccagggtaa
     1381 taggcgcaac gcggcgggtt tcactacgaa accactcccg tgcttcgcgg acttccggca
     1441 gattgtcagt caatacgtcg atacgtcgat gcatatgaat gccagccacg acgtcgggcg
     1501 gaaaactttc ttcaggattt ccgcgtacga aatcagccag caaattgccg gaaagcgagc
     1561 tttccgccag gtgagctaga tgcaggtgag ctaaaaaatt catatacact tcatccttca
     1621 aactgcctct tcgttggctg ccttcactca ccccagtcac ttacttaatg taagctcctg
     1681 gggactcatt cagttgccat ctcgatgcat cttgaatgat tttgtgtata gattatgtca
     1741 tccatcaagg agggtaaagg ttgcagggag agcgccccgg cactagacta cctgcctctt
     1801 attttagtct gagtcagtgt catgcgcgtt accgatttct cctttgaatt gcccgaatcc
     1861 ctgattgccc actatcccat gcctgaacgc agtagctgtc gtttactgtc gctggacggg
     1921 ccgacgggcg cgctgacgca cggtactttc accgatttac tcgataagct caaccccggc
     1981 gatcttctgg tttttaataa tacccgcgtg atcccggcgc gcctgtttgg gcgtaaagcc
     2041 agcggcggca agattgaagt gctggttgag cggatgctcg acgacaaacg cattcttgcg
     2101 catattcgcg cctcgaaagc gccaaaacct ggcgcagaac tgctgctggg cgatgacgaa
     2161 agtatcaacg caacgatgac cgcgcgccac ggcgcactgt ttgaagtcga atttaatgat
     2221 gatcgttcgg tactggatat cctcaacagc atcggccata tgccactgcc gccgtatatc
     2281 gaccgtccgg acgaagacgc tgaccgcgaa ctttatcaaa ccgtttatag cgaaaaaccg
     2341 ggcgcggttg cagccccgac cgctggtctg cattttgacg agcctttgct ggaaaaattg
     2401 cgcgccaaag gcgtggagat ggcgtttgtg acgttgcacg ttggtgcggg tactttccag
     2461 ccggtgcgcg tcgacaccat tgaagatcac atcatgcact cggaatacgc tgaagtgccg
     2521 caggatgtgg tagacgcggt actggcggcg aaagcgcgcg gtaaccgggt gattgcggtt
     2581 ggcaccactt ctgtacgttc gttggaaagc gcggctcagg cagcgaaaaa cgatctcatt
     2641 gaaccgttct tcgacgatac ccaaatcttt atctatccgg gcttccagta caaagtggtc
     2701 gatgcgctgg tgacgaactt ccacttgcca gagtcgacgc tgattatgct ggtttcggcc
     2761 tttgccggtt atcaacacac catgaacgcc tataaagcag cggtagaaga gaaatatcgc
     2821 ttttttagtt acggtgatgc gatgtttatc acgtacaatc cgcaggcaat taatgagcgc
     2881 gtcggggagt aattccgcgg cgctggttta aaacgttgga ctgtttttct gacgtagtgg
     2941 agaaaaaatg aaatttgaac tggacaccac cgacggtcgc gcacgccgtg gccgcctggt
     3001 ctttgatcgt ggcgtagtgg aaacgccttg ttttatgcct gttggcacct acggcactgt
     3061 aaaagggatg acgccggaag aagttgaagc cactggcgca caaattattc tcggtaacac
     3121 cttccacctg tggctgcgcc cgggccagga aatcatgaaa ctgcatggcg atttgcacga
     3181 ttttatgcag tggaaaggcc cgattcttac cgactccggc ggcttccagg tcttcagcct
     3241 tggtgatatt cgtaaaatca ccgaacaggg cgtgcacttc cgtaacccga tcaacggcga
     3301 cccgattttc ctcgacccgg aaaagtcgat ggagattcag tacgatcttg gttcggatat
     3361 cgtcatgatc tttgatgagt gtacgccgta tcctgctgac tgggattacg caaaacgctc
     3421 catggagatg tctctgcgtt gggcgaagcg tagccgtgag cgttttgaca gtctcggaaa
     3481 caaaaatgcg ctgtttggta tcattcaggg cagcgtttac gaagatttac gtgatatttc
     3541 tgttaaaggt ctggtagata tcggttttga tggctacgct gtcggcggtc tggctgtggg
     3601 tgagccgaaa gcagatatgc accgcattct ggagcatgta tgcccgcaaa ttccggcaga
     3661 caaaccgcgt tacctgatgg gcgttggtaa accagaagac ctggttgaag gtgtgcgtcg
     3721 cggtatcgat atgtttgact gcgtaatgcc aacccgcaac gcccgaaatg gtcatttgtt
     3781 cgtgaccgat ggcgtggtga aaatccgcaa tgcgaagtat aagagcgata ctggcccact
     3841 cgatcctgag tgtgattgct acacctgtcg caattattca cgcgcttact tgcatcatct
     3901 cgaccgttgc aacgaaatat taggcgcgcg actcaatacc attcataacc ttcgttacta
     3961 ccagcgtttg atggcgggtt tacgcaaggc tattgaagag ggtaaattag agagcttcgt
     4021 aactgatttt taccagcgtc aggggcgaga agtacca
//
LOCUS       DAYPHN010000247         4032 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000247 DAYPHN010000000
VERSION     DAYPHN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.247"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1619
                     /gene="tssI"
                     /locus_tag="ACG0BW_004587"
     CDS             <1..1619
                     /gene="tssI"
                     /locus_tag="ACG0BW_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFK7261445.1"
                     /translation="DIRTISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLA
                     RLWAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGELPFNPDTSAGAETECVSMFRY
                     EAYVRPSSVQSQDYTFKVPDWPGMYEQQGENLNGQLEQYEIFDYPGRFKDEQHGKDFT
                     LYQMESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGDQPQAL
                     HGSQGRGTTLGNQLEVIPADRTWRPRLQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVR
                     VQFHWDRYNPATEASSCWVRVSQAWAGPGFGNLAIPRIGQEVIVDFLNGDPDQPIIMG
                     RTYHEDNRSPGSLPGTKTQMTIRSKTYKGSGFNELRFEDATDKEEIYLHAQKNMQVVV
                     LNSKDKRVNYDRTVSIGHDESLVVANDRKVTVEGKQDHKTTKDHVSLTEGNQGLEVKG
                     DLAQKISGALGISVQGDIVLQSDSKISLRVGGSFVVIHSGGVDIKGAKINLNGGGSPG
                     DVILPMRPVILKAAAGSGTMFVAHCPKEDK"
     gene            1619..2458
                     /locus_tag="ACG0BW_004588"
     CDS             1619..2458
                     /locus_tag="ACG0BW_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFK7261446.1"
                     /translation="METAPVQYAIVDGAVEEGLLDFLNEVNPPHCCLYAEPIQLDLVA
                     LAPYLVEVVPEVEAWLSVKASPWGIYLTSESPMRELQQHFRRYLWVRIPEQEKPVLMR
                     FYDPRNIWVLVEVLTPRQRLSFISPVRQLSTRYGEECREDNFSSVRPAETMNIRAERP
                     SQLTLSYRQYSQLERKARDNYLDILSAFIEENVDKNGWDDSSKAESSRILAEDYFSFC
                     QSLNIADDRSVRTMTLILLKKNIIDLRYIPDDWYEQLSNQSYPGHIRVHELAQQELGF
                     IPQ"
     gene            2480..>4032
                     /locus_tag="ACG0BW_004589"
     CDS             2480..>4032
                     /locus_tag="ACG0BW_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261447.1"
                     /translation="MTSPSSTAGKPCVECGIPDPCIFDVISDFPEAGEKHFWSKEGVA
                     RFKLLDDGSGCDGIITITYQCDKSGTHEAWLEEESENNHKILQPFGIPNKVTLDDNLS
                     EGEIIAMGTFRVPWDYLGAISSPLKNHYEPAHYSVRIDSCYECGNYVEIDVYPTVEIR
                     FTTGFSYEISSSKQTRTVKERRDERIKARDAMENTKPRDKNKLRSGWTNTTAEFEWIN
                     KTSLTVDFGVKIANVEHTSEYEKEIKKLRQTKQLEQIGRIDSLVKNVNKYFAPDPDNN
                     NGTRKYALFSAKIEPVKLGISYAYQYTDIQDGPCHFYGLFGAPFFEASFRFDIISFIC
                     AYCKVESLVNRCREYLRKNGASVECYIEISAEINLDLGAVYAEKDKKWEFNVKESNIK
                     LGLKGVVSATFEANVFVVQLTAEATASIEAKAGFGFDSHDDGLDLALFHDGIKGKFEF
                     KIDVSHGEVDEKGKGKEKSEKPEKKDTVEWQLCDPMEVKDSPLRVNLYGKERTVEKKV
                     TPPKPVTTWT"
BASE COUNT         1157 a          807 c         1143 g          925 t
ORIGIN      
        1 aggatatccg gacgatatcg gccacgttac tgaacgagaa cggcgtcacg gagtggacac
       61 cgctgtttta tgaggaccat ccggcgcggg agttctgtgt ccagtacggg gaaagcgacc
      121 tggcgtttct ggcgcggttg tgggcggagg agggaatttt cttctttgag cggtttgcgg
      181 cggacagtcc ggagcagaag ctgacgctgt gcgacgatgt ggcggggctg tcacaggcgg
      241 gagagcttcc gtttaacccg gacacatcgg caggagcgga gacagagtgt gtcagtatgt
      301 tccgttatga ggcgtatgtc cgcccgtcgt cggtgcagag ccaggattac acgtttaagg
      361 tgccggactg gccggggatg tatgagcagc agggtgagaa cctgaacgga cagctggagc
      421 agtatgagat atttgattat ccgggaaggt tcaaggatga acagcacggc aaggatttca
      481 cgctgtacca gatggagagc ctgcgcagtg atgcggaaaa ggccacaggg cagagtaatt
      541 caccgaagct gtggccggga acgcggttta cgctgacggg gcatccgcag aagatgctga
      601 accgtgaatg gcaggtggta cagagtattc tgtcagggga ccagccgcag gcgctgcacg
      661 gcagccaggg gagaggaacc acgctgggaa atcagttaga ggtgataccg gcggaccgga
      721 cgtggcgtcc acggctgcaa agcaaaccga aggtggacgg gccacagagc gccattgtca
      781 ccggtcctgc gggagaggaa atcttctgcg acgaacacgg acgtgtgcga gtgcagtttc
      841 actgggacag gtataacccg gcaacggagg cgagttcatg ctgggtgagg gtgtcgcagg
      901 cgtgggcggg gccgggattt ggtaacctgg cgataccgcg catcggccag gaagtgattg
      961 tagacttcct gaacggcgac ccggaccagc cgataatcat ggggcggacg taccatgagg
     1021 acaaccgttc gccggggagt ctgccgggga cgaagacgca gatgacgata cggtcgaaga
     1081 cctataaagg aagcggcttt aatgaactgc ggttcgagga cgcgacggac aaggaagaaa
     1141 tttacctgca cgcacagaaa aatatgcaag tcgttgtgct gaacagcaag gataagcggg
     1201 tcaactatga ccggacggtc agcatcgggc atgatgaatc cctggtggtg gcaaatgacc
     1261 ggaaagtgac ggttgaaggt aaacaggacc ataaaaccac caaagaccat gtcagcctga
     1321 cagagggcaa tcaggggctg gaggtgaaag gcgatctggc acagaaaata agcggcgcgc
     1381 tgggtatcag cgtacagggc gatattgttt tacagagcga cagcaaaatc agtctgcggg
     1441 tgggcggttc atttgtggtg atccattccg gcggcgtgga tattaagggc gcgaaaatca
     1501 atcttaacgg cggaggcagt ccgggagacg tgatcctgcc gatgcgcccg gtgatcctga
     1561 aagccgcagc cggaagcggg acaatgtttg tggcgcactg cccgaaggag gataagtgat
     1621 ggaaacggca cccgtgcagt acgccattgt ggatggcgct gtggaggaag gattgctgga
     1681 ttttctgaac gaggtgaatc ccccgcactg ttgtctgtat gcagaaccca tacaactgga
     1741 tctggtcgcg ctagccccct atctcgtgga agtcgttccg gaggttgaag catggctgag
     1801 tgttaaggcg tcgccatggg gaatatatct gacttctgag tcacccatgc gtgagctaca
     1861 gcaacacttc agacgttatc tgtgggttag gatccccgaa caggagaaac ctgttctcat
     1921 gcgattttat gatccccgga atatatgggt attagtggaa gttctgaccc cccgccagcg
     1981 tctttctttt atcagtcccg tcagacagct aagtacccgt tatggtgaag aatgtcgtga
     2041 ggataatttt tcttccgtcc gtcccgcaga aactatgaat ataagggcag aacggccatc
     2101 acaactgacg ctgagttaca gacaatatag ccaactggaa cgaaaggcac gggataatta
     2161 tcttgatata ttatctgcat ttatcgaaga aaatgtagat aaaaacggat gggatgattc
     2221 atcaaaagct gaatcatccc gaattctggc agaagattat ttctcttttt gccagtcatt
     2281 aaatatagca gatgatcgtt ccgtcagaac aatgacgctt atattactga aaaaaaatat
     2341 aatcgatctc agatatattc cggatgactg gtatgaacaa ctcagcaatc agtcataccc
     2401 gggacatatt cgggtacatg aacttgcaca acaggaactg ggatttatcc cacaataaaa
     2461 agacaaacgg atagattata tgacatctcc atcttcgaca gccggaaagc cctgtgtgga
     2521 atgcggcatt cctgatccat gtatttttga tgttattagt gattttccag aggccggcga
     2581 gaaacatttc tggtcaaaag aaggagtggc gcgttttaag ctactggatg atggttccgg
     2641 atgtgatggt ataattacca ttacctacca gtgcgataaa agtggaacac atgaggcatg
     2701 gttagaggaa gaatcagaaa ataatcataa gatactgcaa cctttcggaa tacctaataa
     2761 ggtcactttg gatgataact tgtctgaagg tgaaataatt gctatgggta catttcgagt
     2821 accatgggat tatttggggg caatatcctc tccacttaag aatcattatg aacctgccca
     2881 ttattcagtc agaattgaca gttgttacga atgtggcaat tatgttgaaa ttgatgttta
     2941 tcctacagtg gaaattaggt ttactacagg gttcagctat gagatttcgt catcaaaaca
     3001 gacgagaaca gtaaaagagc ggcgtgacga aagaataaaa gccagggacg caatggagaa
     3061 caccaagccc agggataaaa ataaactccg tagcggctgg acaaatacca ctgctgagtt
     3121 tgagtggata aacaagactt ccctgactgt cgattttggc gtaaaaatag ctaatgttga
     3181 acacacaagt gaatatgaaa aagagataaa aaaacttcga caaacaaagc aactggaaca
     3241 gataggaaga attgactctc tggtcaagaa tgtcaataaa tattttgcgc cagatcctga
     3301 caataataat ggtacgcgca aatatgcatt gttcagtgca aaaatagaac ccgttaaact
     3361 gggtatatcg tatgcctatc aatataccga tatccaggat ggcccctgtc atttttatgg
     3421 tttgtttggt gccccatttt ttgaagcgtc gttcagattc gatattattt catttatatg
     3481 tgcatattgt aaagtcgagt cattggtcaa cagatgtcgt gaatatctcc gtaaaaacgg
     3541 agccagtgtt gaatgctata tcgagatttc agcggagatt aatcttgatc ttggcgcagt
     3601 atacgctgaa aaggataaaa aatgggagtt taatgtaaag gaaagtaaca tcaagctcgg
     3661 tttaaaaggt gtggtgagtg cgacgtttga ggcgaatgtt tttgttgtac aactgaccgc
     3721 cgaagcgact gcctctatag aagcgaaagc tggatttgga tttgattcac atgatgacgg
     3781 actggaccta gcattatttc atgacggcat taaagggaag tttgaattta agattgatgt
     3841 gtctcatggt gaagttgatg aaaaaggaaa ggggaaggaa aagagcgaaa aacctgagaa
     3901 gaaagatacc gttgagtggc agttatgtga tcctatggag gtaaaggatt caccattacg
     3961 ggttaattta tacggtaaag aacgcactgt ggagaaaaaa gtaacaccgc cgaagccagt
     4021 gacaacgtgg ac
//
LOCUS       DAYPHN010000248         3970 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000248 DAYPHN010000000
VERSION     DAYPHN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.248"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..217
                     /locus_tag="ACG0BW_004590"
     CDS             <1..217
                     /locus_tag="ACG0BW_004590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261448.1"
                     /translation="HIGKHMIKIYLLAVATGLSVALIYGLLVPSLISTKSDLAVMFGV
                     IVGFGAPVIGLIAGRKFINSLIKAKGK"
     gene            221..1051
                     /locus_tag="ACG0BW_004591"
     CDS             221..1051
                     /locus_tag="ACG0BW_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFK7261449.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            1147..1971
                     /locus_tag="ACG0BW_004592"
     CDS             1147..1971
                     /locus_tag="ACG0BW_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261450.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            2019..2759
                     /locus_tag="ACG0BW_004593"
     CDS             2019..2759
                     /locus_tag="ACG0BW_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261451.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPTVSITKMESGKELLP"
     gene            2780..3169
                     /locus_tag="ACG0BW_004594"
     CDS             2780..3169
                     /locus_tag="ACG0BW_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261452.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            3231..3656
                     /locus_tag="ACG0BW_004595"
     CDS             3231..3656
                     /locus_tag="ACG0BW_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261453.1"
                     /translation="MAAIKKLYDAANAALDVIDDEVSKGFPEPDWAHQLRNAIAEMTP
                     SDPTPDETNWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            3712..>3970
                     /locus_tag="ACG0BW_004596"
     CDS             3712..>3970
                     /locus_tag="ACG0BW_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261454.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRILVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILS"
BASE COUNT         1282 a          796 c          904 g          988 t
ORIGIN      
        1 ccacatagga aaacacatga tcaaaatcta tctattggca gtagccacag gcctttcagt
       61 ggctctcatc tacggtttac tggttccgtc gctgatttct accaagagtg atttagccgt
      121 catgtttgga gttatcgttg gttttggtgc tcctgtaatc ggtcttattg ctggtcgtaa
      181 gtttatcaac tcattaatca aagcaaaggg gaaataagta atgaagaaag gtttacttgc
      241 agttgctctg gcggctattt gcacaatggg tcttactggc tgtgatcgcg tggagcctgg
      301 atacgttggc atcaaagtaa acaaattagg tgaagacaaa gggatcggtg aagtggttgg
      361 cgttggtcgc caatggacag gtcttaacac cgaactttac gtattcccga ccttcaaaca
      421 aatgaagacc tacgacgaac cgttcacatt ccagatgagt gacggtactg ctattggtca
      481 caaaattggc gttgcgtatc tggttaatcg tgacaaggta acgacggtgt tccagaccta
      541 tcgcaaaggc gtagacgata tcaccgaatc agatctgcgt cagaaaattg ccgactctct
      601 aaaccgtttg gccagccgta tgaccactga ctcatttatc gacggtggta aggcgcaatt
      661 gctggacaac gcactgaaag acattcagaa agagatgtct ccggttggta ttgaggtact
      721 gagcctgtca tgggttggaa agcctgatta cccaaaaacc gtcattgaat ctatcaacgc
      781 caaagtaacg gctaaccagc gtactctgca acgtcagcag gaagttgaac agcgtaaagc
      841 tgaggcgaat atgctacgtg aacaggctaa tggtgaagct gatgctatcc gtgctcgtgc
      901 gcaagcagaa gccgacgcca ttcgtctgcg cggtgaagct ctgcgtcaaa acccgaacgt
      961 tatggagctg gaagccatca ataaatggaa tggccagtta ccgcagtaca tgactcaagg
     1021 ggctaacact cctttcatta cagtgaaata actctcatta agaagatcag gcgtccagtt
     1081 ggacgccgtt ttattctcgt tattatcaca cacaagaaaa caatttgtta aaaggttaag
     1141 ttaattatgc ccaaccacat tacaaacgaa atccgagtca taggtggtac aaataaacaa
     1201 cgcttagatt tcatacgtgc cataactaac aaaaaaggtc taattgactt taataccatt
     1261 tgcagacgtc cgaaaagtat agaaatggaa gaaaacaatc atatcaggcg catggcctct
     1321 gcaatggcgg gcgaaacagt ttacgaccac atgttcggtg aattaaagac tccagaacag
     1381 gtcgaattag ctatgcgtga atcaggaatg acacgcaagg aaattcgcaa aatcaaagag
     1441 caggctctta tgcggcttga gaattatcgt cgctatggct attactcatg gtttgactgg
     1501 tcgcgagcta attggggaac aaaatggaat gcctacaaca tcgaaatgcc cgtaaaacgt
     1561 atccctacac gtattaagta cggccataat ttccgccata cacacgttcg agcatatgca
     1621 aaaagaatct ataaaaaacg tcttgcaaga catgccgcaa ctggtggtga gttggtgatt
     1681 cgctttgaaa cagcctggtc tatgccaaaa ccaatttatc aagcgatggc tagaaagttc
     1741 ccgcaccttg agtttgtaat acgttatgca gacgaagact tcggcaataa ctgcggattc
     1801 attttacttc gcaacggcaa atgggaatat gactacattg caccggcgaa taacaaacaa
     1861 tcgtcagaag aaaaaatcaa atggcgcaag ttcgcctttg agttatgttg tcctggtatc
     1921 actccacaag aatacggtct aaatgaagaa tacgaatacg ccggatatta aaaaaaattg
     1981 tcatttagct ttacgcatga agcatagagg ttaataagat gaaaagaaaa attgaagaac
     2041 caaaaacgac ccttaataaa catggggaaa aaattcaaac acatccagcg ttcggtcttg
     2101 tgaaaacaag ccgtgttagc acaactggca ttcgcctatt tgattcagaa ctggaacata
     2161 gagactatat tgaaataggc gtttacgagg cggaactgtc cttagacaat gaacgaaccc
     2221 gcccacaaag aagttgcaat agacggcctt tagttgaaat tagacttagc caggcacaat
     2281 gggctgctat ggttagcagt tttggagtgg gcgaaggtgt gccatgcact atttcatata
     2341 aaaccattgg agactcaatt agagttcatg atattgccga gcaaaaaagt attcggcaaa
     2401 aattcaacga tcaaataaag gccgctacga atagtgagat tgatcgactt aatcagcaag
     2461 tttcagagct aggtgaactt gtgaaaaaag gaagagctgg aaaacgtgac cttgaagaac
     2521 tatatagaaa actagcatct gctgtagcta accttcctga aaatctgtgc tttgctaccg
     2581 aattaatgca ggaacaaatg gatgaaattg tctcatctgg aaaggcggag attgaggcgt
     2641 atgttgcggg cacggttatg cgtaaagggt tagcagcatt aggcacagaa tcatgcatta
     2701 acgatgatcc tactgtctct atcaccaaaa tggaaagcgg taaagaatta ctgccataaa
     2761 tgcatctgag gtactgacaa tgcttttaat tcaacctggg ttcggtttaa agataaaaaa
     2821 agggcatatg tttggtgagc gagattccaa acgaaaaatt ctgtctattc gattaccatt
     2881 tatcaccatt cattggttaa acagagagtc aaccgattat tggtataaat gcgcaagagc
     2941 agcatttaac gaccctgact ggtttgtaga aaatcaccac gcggttcgtc aggcaaaaag
     3001 aaaggctaat acgacatata tgaaggcata tcaggatgcc tgggaagaac atcgtggccg
     3061 ataccaaaaa aacattgaaa aacttgaatc agaaaatatg gacctaagaa gaaaactcgg
     3121 tgaagcacgt cgagacatcg aagcatataa acgtcttgta tcggagtgaa tatatctttg
     3181 ctttattttt tcaaaggtga ttattttcct atttataagg agaagtaaac atggcagcaa
     3241 ttaagaaact ctacgatgcc gcaaatgcgg ctctggatgt tattgatgat gaagtatcaa
     3301 aaggctttcc tgaacctgat tgggcgcatc agctacgaaa cgctatcgca gaaatgaccc
     3361 catcagatcc aacccccgac gagacaaact ggcagcgatt catccgtatg tacgctcagg
     3421 aaataggtcc aacgccaacg gcagagcagg caatgctgct gaaatacttc aaagaggcgg
     3481 gagaggattt accaattgat gactcagcat attggttcca ctgcgcatgg cgtaagtatg
     3541 acgtgatatt cacacaaggc atgggaagca aagatatggt tgtgtggcat ctactccata
     3601 tagacacagc cgttgacaga gttattgaac agtttttccc taaccaagaa gattgatcgc
     3661 ctattcataa ctaacaaaat aagtaaacac taaccacaaa aggaaaaaca catgagagtt
     3721 ttagttcgaa tcgttaccag cactgtctat gacgtgtttc cgctttttat ggtcaaagtc
     3781 gatggcctta acgatgaaga aactgacgcg ctgatccagc gtattctcgt tgaatataca
     3841 ggtcatgacg ctgattcagt gatggttgat gatgatggtg tttgttggca taacggcaac
     3901 tgttggtacg tagaagagac tcaacaaatc agtgatgaag atgccgcaca tcttgagcgt
     3961 attttaagca
//
LOCUS       DAYPHN010000249         3962 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000249 DAYPHN010000000
VERSION     DAYPHN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.249"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            263..784
                     /locus_tag="ACG0BW_004597"
     CDS             263..784
                     /locus_tag="ACG0BW_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFK7261455.1"
                     /translation="MWTVSLAEAFIHVFQVAGKIFLDMMTGLIPMLICLLLAINFLMK
                     LVGTVRMEKVAALLGRSRILTYGVLPVLGWFFMSSPGALTLGKLLPEKSKPGYEDALG
                     TPAHPLTSLFPHVVPSELFVWLGVAAGVKALGLPVTSLALRYIAAAMLIGLIRGIITE
                     YLFLRLSKRGNAP"
     gene            802..1155
                     /locus_tag="ACG0BW_004598"
     CDS             802..1155
                     /locus_tag="ACG0BW_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFK7261456.1"
                     /translation="MVAIGLFVADGLTDKMLITFDSNGPKDCLDYSLSLEPSFREESL
                     MILPGDRLLLAGHDFLVTAVGKGAQQALFELGHLTLVFNGDLNPCHVGAVHLSGPVPN
                     LRDLHGNLVIEEGRP"
     gene            1152..2132
                     /locus_tag="ACG0BW_004599"
     CDS             1152..2132
                     /locus_tag="ACG0BW_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HFK7261457.1"
                     /translation="MNTVLIPPGPGGYGKGLWLPTGSGKKVLSLTGREIHPVAIEIGA
                     LTESEVVNGFADIPPDNDILCVVINCAGSLRCGLYPQKGIPTINVLPTWRAGPLAHFI
                     SEENYVSGVTIEQLVLVDTAEAPDCQPESVPAPEPVITTAPPPPRGAEKLVRMVEQTG
                     TAVGHVIALLFAASREAVDVSLRNVIPFMAFVSLLIALVQETALGSLIAHALTPLANS
                     LWGLVLLSLIRGIPFLSPVLGPGAAISQVIGVMIGTQIGAGAISPAFALPALFAINVQ
                     VGCDFVPVGLSMQEAKPETIAKGVPAFLLSRQLTGPLAVIVGWLFSLGLF"
     gene            2143..3156
                     /locus_tag="ACG0BW_004600"
     CDS             2143..3156
                     /locus_tag="ACG0BW_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFK7261458.1"
                     /translation="MNKIRAAVVGAGIYGKHHMNAYLHNPDTVLVAICDTDTERCDDL
                     AMTCGVMGYTRLDVLLQEEAIDVVSVATPDPYHTESILTALRHGKHVLAEKPLATSVR
                     ECELIVEMAQQRGLLVGVDFHKRWDPAVMRIKAELEKPETGRILRGHISMDDVITVPT
                     DWLDWAGASSPVWFLGSHCFDLVRHLSGQEVVSVYAAGQKRLMTECGLDTFDSVQSLL
                     TMADGSSWVVENSWVLPEGFPKDNDGRIDILCEATYIRSTSQHRGLEITTPGMTLTPN
                     SYFINYRNGVASGFGIDPINDFVRAVRNRSPYPVTAEDGLAVSRICEAVHRSLESGKP
                     EDV"
     gene            3450..>3962
                     /locus_tag="ACG0BW_004601"
     CDS             3450..>3962
                     /locus_tag="ACG0BW_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexulose-6-phosphate synthase"
                     /protein_id="HFK7261459.1"
                     /translation="MLEFHNVPLKTILRRAIMSLPTNFNDILRLFEKDYDTAKEDNAL
                     SARGQFLQLYPLNRLKKMTLDDYVIGKGTASFCACVEVKTRTWANMQGATALKFGIYY
                     GKSKSDPAVRYRFTQKFGDDDSTNKEVFANVKDALLDLIQSGKELDFRAIDENPLSQM
                     FKAKILSLYFP"
BASE COUNT          842 a         1136 c         1083 g          901 t
ORIGIN      
        1 atctgtacac attgcagaca atcaccctct gcaaacccaa ctgctgagca tcgcatactc
       61 agggcagaat tgccacgcat ccgctcgtcc tgaaaaccgc tcagttcaca aaaacacgcc
      121 acaagcgggg ataacaaaga aagcagtatg agccatagct gtttatcgcc attaccggag
      181 ccccagacgc ttacgcctgt catgtcttaa cctctgtccg ataagtctgt gcgctccggg
      241 ccttttaccc ggaggactcc ttatgtggac agtcagcctc gctgaggcgt ttattcatgt
      301 attccaggtc gcaggcaaaa tcttcctcga catgatgacc gggcttatcc cgatgctcat
      361 ctgcctgctg ctggccatca acttcctgat gaagctggtt ggtaccgtgc ggatggagaa
      421 agttgccgcc ctgctcggcc gctcgcgcat tctcacctac ggcgtgctgc cggtgctcgg
      481 ctggttcttt atgagcagcc ccggcgcgct cactttaggc aaactcctgc ccgaaaagag
      541 caagcccggc tatgaggatg cgctcggcac ccccgcccat cccctcacca gcctgtttcc
      601 ccacgtagtg ccatcagagc tgtttgtctg gctcggcgtg gctgccgggg ttaaggcgct
      661 cggcctgccc gtcacctcgc tggcgctgcg ctatatcgcc gccgccatgc tgattggttt
      721 aatccgcggg attatcaccg agtacctgtt cctgcggctc agcaagcggg ggaatgcgcc
      781 atgatgattt actgagcacg tatcgtcgcc atcgggctgt ttgtcgccga tggtctcacc
      841 gacaaaatgc ttatcacctt cgacagcaac gggccgaagg actgtctcga ttactcgcta
      901 tcgctggagc cgtcgttccg ggaagagagc ctgatgatac tccccggcga ccgcctgctg
      961 ctggcgggcc acgattttct ggtgactgcc gtcggcaaag gggcacagca ggcgctgttt
     1021 gaactcggcc acctgacgct ggtgttcaat ggtgacctga atccctgcca tgtcggcgcg
     1081 gttcatctct ccggcccggt gccgaacctg cgcgacctcc acggcaatct ggtgattgag
     1141 gaggggcggc catgaacacg gtgctcattc cccccggtcc cggcggctat ggtaaggggc
     1201 tttggctacc cacagggagc ggcaaaaagg tactgtcgct gaccgggcgc gagattcatc
     1261 cggtggcgat agaaatcggc gcacttaccg aatcggaagt ggtgaatggc tttgccgata
     1321 tcccgccgga taacgacatc ctgtgcgtgg tgattaactg tgccgggtcc ctgcgctgcg
     1381 gcctgtatcc gcagaagggt atcccgacca tcaacgtgct gcctacctgg cgggccggac
     1441 cgctggcgca ctttattagc gaagagaact acgtctccgg tgtgacgata gagcaactgg
     1501 ttctggtgga taccgccgaa gcgccggact gtcagcctga gtcagttcca gctccggagc
     1561 cagtcattac caccgctcct ccacctccgc gcggtgcgga gaaactggtg cgcatggtcg
     1621 agcagaccgg caccgccgtc ggccacgtta tcgccctgct gttcgccgcc agccgtgagg
     1681 cggtggacgt gtcgctgcgc aacgttatcc cgtttatggc ctttgtctcg ctgctgattg
     1741 ccctggtgca ggagaccgcg ctcggcagcc tgattgccca cgccctgacg ccgctggcga
     1801 actcactgtg gggactggtg ctgctgtcgc tcatccgcgg cattccgttt ctctccccgg
     1861 tgctagggcc gggtgcggct atctcgcagg tgattggcgt gatgattggc acacagattg
     1921 gcgcaggggc gatatctccg gccttcgccc tgcccgccct gtttgcgatc aatgtgcagg
     1981 tcggctgcga cttcgtgcct gttggccttt cgatgcagga ggcaaagccg gagaccatcg
     2041 caaaaggtgt acccgccttt ctgctgtcga gacaactgac cggaccgctt gcggtaatag
     2101 tcggctggct gttttccctg ggcttattct gaggacataa cgatgaacaa aatcagagcc
     2161 gccgtggtcg gcgcaggtat ctacggcaaa caccatatga atgcgtacct ccataacccg
     2221 gacaccgtgc tggtcgccat ctgcgacacc gatacagaac gctgcgacga cctcgccatg
     2281 acctgtggcg tgatgggtta tacccggctg gatgtcctgt tacaggagga agccatcgac
     2341 gtggtctcgg tggcgacacc tgacccgtac cacactgaat ccatcctcac cgccctgcgc
     2401 cacggcaaac atgtgctggc ggagaaaccg ctcgccacct cagtacgcga gtgcgagctt
     2461 atcgtggaga tggcgcaaca gcgcggcctg ctggtcggcg tcgatttcca caaacgctgg
     2521 gacccggcgg tgatgcgcat caaagccgaa ctggagaaac cggagaccgg gcggatcctg
     2581 cgcggccata tcagtatgga cgacgtgatt accgtgccga cggactggct ggactgggcg
     2641 ggcgcaagct cccctgtgtg gtttctcggc tcacactgct ttgacctggt gcgccacctt
     2701 tccggtcagg aagtggtgtc ggtgtatgcc gccgggcaga aacggctgat gaccgaatgc
     2761 ggcctcgaca ccttcgacag cgtgcagagc cttctgacga tggccgacgg cagttcgtgg
     2821 gtggtggaaa actcgtgggt gctgccggag ggcttcccga aggacaacga cgggcgcatt
     2881 gatattctct gtgaggcaac gtatatccgc agcacctcgc agcatcgcgg gctggaaatc
     2941 accacgccag gcatgacgct cacgcccaac agctacttca tcaactaccg caacggtgtc
     3001 gccagcggtt tcggtatcga tccgataaac gactttgttc gcgcggtcag gaaccgttcg
     3061 ccgtacccgg tgacggcaga ggacgggctg gcggtcagcc ggatttgcga ggcggtgcac
     3121 cgcagtctgg agagcgggaa acctgaagat gtttaaacaa acggcgcagg gtagctccct
     3181 gtgccagttc catatgattt tgctaattct actcactggt atattcactc gttaggcttt
     3241 ggcccgcaga tatattaatg ttttccatcc tgccgcaaca aaaccccaca cacgattgta
     3301 tgttttttag tcatcaaggt gaatagaggt attttatggc tatgaccttt ttcacctccc
     3361 tgccaatagt atcatgctaa acctctcatc tatgtttatt gcgccataat agtcaacaag
     3421 tatttcaaaa aagtcccaag caaattataa tgctggagtt tcataatgtc cctttaaaaa
     3481 ccattttaag aagagctatt atgtccctcc ctacaaattt taatgatatt cttcgtcttt
     3541 ttgaaaaaga ttacgataca gccaaagaag ataatgcctt aagtgcacgc ggacaatttc
     3601 tgcaacttta cccccttaat cgcctaaaaa aaatgacgct tgatgactat gtcatcggca
     3661 aaggcacggc ctcattttgt gcttgtgttg aagtaaaaac cagaacatgg gcaaatatgc
     3721 aaggtgcgac ggcgctcaaa tttggtattt attatggaaa atcaaaatca gatccagccg
     3781 tccgctatcg ttttactcag aaatttggcg atgatgatag tactaataaa gaagttttcg
     3841 ctaatgttaa agacgcttta ctagacctaa tacagtcagg gaaagaatta gattttagag
     3901 cgattgacga gaacccccta tcccaaatgt ttaaggctaa aatattgagc ctttactttc
     3961 ca
//
LOCUS       DAYPHN010000250         3897 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000250 DAYPHN010000000
VERSION     DAYPHN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.250"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..552
                     /gene="ykgH"
                     /locus_tag="ACG0BW_004602"
     CDS             <1..552
                     /gene="ykgH"
                     /locus_tag="ACG0BW_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFK7261460.1"
                     /translation="LYINKDNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVI
                     QRITTFFQTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVL
                     DEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFIS
                     IVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(795..1490)
                     /locus_tag="ACG0BW_004603"
     CDS             complement(795..1490)
                     /locus_tag="ACG0BW_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFK7261461.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRIAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1483..2910)
                     /locus_tag="ACG0BW_004604"
     CDS             complement(1483..2910)
                     /locus_tag="ACG0BW_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFK7261462.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2921..3640)
                     /locus_tag="ACG0BW_004605"
     CDS             complement(2921..3640)
                     /locus_tag="ACG0BW_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFK7261463.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1029 a         1034 c          817 g         1017 t
ORIGIN      
        1 ctgtatatca ataaagataa tataaaagtg agttacagcc taaaaataaa ccagacaaca
       61 ccaggcatac ttgttagctg tgatagcaat aataactttg cctgtcagac cacaatgact
      121 gaagatgtta ttcagcgaat tactacattt tttcagacca gcccagatgt caagaacaga
      181 gaaataaagc tggaatggtc aggagataag agagatttac caactgctga agcggaaata
      241 tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aatatcataa tggcaggcaa
      301 gttctcgatg agatacaaac accttcagca attaacagtg agctttatac aaaaatgata
      361 tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac tataagctca
      421 ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt ttttataagc
      481 attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata ccaacaaaac
      541 tctgggcagt aataaacagc attctgacag agagtgcctg tataaaacta tagtgacaat
      601 gaccgggaag ggagcccgtc ttcaggaatg gattcctgtc tttttcactg taaatatcct
      661 ggcattacta atgccaggat atttacagaa gagcagcata accatgacgg aacggggtta
      721 agtcaggttg tccttaacgc tattcacaac ctcgtatgcc gacttcaggt ttatgtccag
      781 acttcatatc actctcagca atcctcaata atcagataca ccgctttcac cggaccatga
      841 accccgacga ctttgataag ctcaatgtcc gccgttgaac tgggaccact gatgatgtta
      901 atacatgacg gcattcgttc accggcctgc gctttctgat gcaatttttc tgcaagttgc
      961 gctatacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg
     1021 ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa
     1081 ccatattcag catacacgac acccacttta gcctgctctg cctgcgagat attctcggca
     1141 cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc gctaatcccc
     1201 aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac
     1261 agacgtattg cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata
     1321 acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag ttgggtaagc
     1381 cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcaacggg
     1441 cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc
     1501 ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc
     1561 gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc cgccattgat
     1621 aaaccagctt gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact
     1681 attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc
     1741 agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac
     1801 gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc
     1861 gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg
     1921 accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg
     1981 cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag
     2041 atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggtcctgtca gccaggtgtt
     2101 gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac
     2161 ctcggcaaac gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag
     2221 cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac cggtctctgc
     2281 caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt cgcggatttt
     2341 ttgccgaatg aataaggtca ttgcttcagg cgtttccggc ccctcatagc ccagacgttc
     2401 gtgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga
     2461 gggcggatct tgatccagct ggagaatgta ttcacccaga tcggtttcaa tcacctgaat
     2521 gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt
     2581 caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atatagcggg tggcgtcttc
     2641 tttggttttt gcaaaataga cgtgaccgcc gttttgtgtc actttttctg agagctggta
     2701 cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc gatcgcgcca
     2761 ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg
     2821 cgcgtttgcc accgctttgc gcatgatagg atcttcaatt tgctgacgga tgcgtgtctt
     2881 aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc atcagcactt
     2941 cagcaatatg cattactttg actttctgcc cttcccgttg taatcgccca ctgatgttca
     3001 gcaggcaact cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcgcaa
     3061 ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa
     3121 atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca
     3181 gcagcgtaag tggttcgtcc ttcaccccca gcttacgggc caggctacaa gatgggtgat
     3241 acaccgctct cccttgcaaa ctggcaccta catcgactac ccctaatgta ttaacaataa
     3301 aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt
     3361 catccgccag atacgtcggg taacttttta cggcataggt gcaagagcca gccggtgaaa
     3421 taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt
     3481 ctttgatata accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat
     3541 ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca
     3601 gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc
     3661 atacccattt atgcccgatg gttcccccct tccgggggat caatcatcaa taaatatggc
     3721 gttctttttt aattggctag taacatacca cccctcattt gtatgacaaa tgataatctt
     3781 tcatctaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat
     3841 agggaaatcg acgatcattt ttagcataag aaatccattt aaaaaggcta catccct
//
LOCUS       DAYPHN010000251         3772 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000251 DAYPHN010000000
VERSION     DAYPHN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.252"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(657..755)
                     /locus_tag="ACG0BW_004606"
                     /pseudo
     CDS             complement(657..755)
                     /locus_tag="ACG0BW_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
     gene            complement(845..1213)
                     /locus_tag="ACG0BW_004607"
     CDS             complement(845..1213)
                     /locus_tag="ACG0BW_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFK7261464.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(1376..1597)
                     /locus_tag="ACG0BW_004608"
     CDS             complement(1376..1597)
                     /locus_tag="ACG0BW_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFK7261465.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(1660..2136)
                     /locus_tag="ACG0BW_004609"
     CDS             complement(1660..2136)
                     /locus_tag="ACG0BW_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFK7261466.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            complement(2152..2625)
                     /locus_tag="ACG0BW_004610"
     CDS             complement(2152..2625)
                     /locus_tag="ACG0BW_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFK7261467.1"
                     /translation="MKTLSQNTTSSACAPETDLQKLVATPVPDERRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(2967..>3772)
                     /locus_tag="ACG0BW_004611"
     CDS             complement(2967..>3772)
                     /locus_tag="ACG0BW_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFK7261468.1"
                     /translation="RFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRYAYIPT
                     ITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIILLNSHD
                     GTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEK
                     HDAMQSLVLSPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGQDLWSAYQTI
                     QENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          899 a          951 c         1038 g          884 t
ORIGIN      
        1 acatgtagat tttttgaact gtataaaatt aaaaatgatg tcgtatggag tcatgatcct
       61 tacaggaatc agcagaggaa aacatctatt attaccatgt aattcatgtt gttatgtcgc
      121 aacaatagta ctgcacgggg aattattacg ggcaaactta ggcgaaagga agtactgaag
      181 tgacagaata aatcacactt tttgtacata tgtacttttc tattgtcttt tgttgtggaa
      241 acaaatggtg tttgtcatgc cgatgctaat agacagatag attcgagggc caacagcccc
      301 atcatcatga tggagcaaca gaggtgagtc taacgtttcc gcacttctta tcgatcggca
      361 ctaacgatca atatcgataa actgatctat caaaacgatt atctttcttg caacctgcct
      421 agttatcctt ctctcacatt gctcgcagaa aagccctttt attacggtta gttatctagc
      481 tgaggcttgc tacgcccatt ttccatgtac cccaaatcac gaggtcacaa aggcgcggaa
      541 agatgaaggg atgtgcacgt tgcaacaaaa aaaagaagcg tccttgaaca gaggctctca
      601 ctcgataaag aattctgcga cgagtgctta ttcgatatcg atgaggaaat cagggaatgc
      661 tggtccagta accgggtgag cagtgtctgc cagatggtga ctggagacgg gcagcacgat
      721 acctcccgta catgcgtgtc gggtaatgtt ttcatactga agattttcct gatatgcaga
      781 gataaaaaat gggacagtgg cgtggtgaaa acaccaggcc ggagcagaag gttattctgg
      841 tggtttactt tttcgtttca ggtgtcggat aaacagccag ataaacgtaa ccacaggagc
      901 cgagggtgtc ggcttcacag gtcagcccct tcgcatacag tgtcacggta tgctgatggc
      961 ggggattcag ttcaccactg gtgagcatta atttcagttg tttcatcagc agcggaaagg
     1021 cctggtccag agggtacgca tccgcatctc tgaaccggcc tctgataccg gcacggtcgg
     1081 caaggtaatg caaccggtta ccctcctgca ccagacgggc gccgaaacag ggcgtcactg
     1141 tgcagggtag tccccaccag ggatggtcgt cattgtcatc gggatgcgtt gtcccggaaa
     1201 gtgtgtctga cacgataaaa tcctcacaga aaatcggtga atgatagttt aacgatgacg
     1261 ggtcagccgg cgcgcagacc ggcttcggta aaggtgtatt cgttgatgag caggacctca
     1321 tccacagcgg cgtctgagct ctgccagtcg tactcgtttt ccgggtgacg gaacatcagt
     1381 tcagggtgac gctcatcagc cggacatacg gaccaaaact gtccttacgg cgttctgcaa
     1441 acacggccag cacaccggga atatcctgca cttcacgacc ggtatacgct tcagcactgc
     1501 cgtgccagcg gtatttgccg gtacagaacg gaaaaagacg ggatgccgga tgctgacggt
     1561 gaatacgcat ggcttcgcca cgggtgatga ttttcatagt gggatacctc tgaagacaga
     1621 agataaaagt gaaaacaggt gtgatgtggt tgtgacgggt taaagcagac catattcggc
     1681 aaaggagaaa acctggctgc caccgactat cagatggtcc ggcacccgga tatccaccag
     1741 gcccagtgcc tgtaccagac gttccgtgat aaggcggtct gccttgctgg gtgtgacttc
     1801 accggacggg tgattgtgtg ccagcaccac agcagcggca ttgtgataca gggcgcgttt
     1861 aatcacttcc cggggatgga cttccgtgcg gttgatggtg ccggtgaaga gggtttcacc
     1921 ggcaatcagc tgattctggt tgttcagata cagcacccgg aactcttcac gctccagtcc
     1981 cgccatgttc agaatcagcc attctcgtgc cgcacgggtg gaggtgaagg ccacgccggg
     2041 ttcatgaaga tggcggtcca gggttttcag ggcccgttga atgaggctgc gctcgccggg
     2101 cgtcatctct ccgggcagaa aggaaagctg ttgcattgta cttctctcca ttcagtcgat
     2161 gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca gccggtaata
     2221 atggaccgtc atggcataac actccgtacg acaggcatga tgactgtacg tcatcagaca
     2281 ggcagcaatg ccggcggctt ccgggctcat ttcagcgcgg ttaccgttca tggcattgaa
     2341 cagtacccat gtttcgtcat catcgtcatc cggttcgggt gccataaatg ccccgccgtt
     2401 gttcagggtg tacagattcc agataccacc gcagtagtct tcacacagac ggtccatcca
     2461 gccgaagata cggggctcca gggtcaccca ctgtggaatg aggccaaagt gctgcggcca
     2521 gaagctgatg cgccgttcat cagggacggg agtggctacc agtttttgca ggtcagtctc
     2581 cggtgcacag gccgaagagg tggtgttctg agacaatgtt ttcatgtatg aaatccttgt
     2641 aatgtgaata aatgagaggg agtaaaggtc gggagctggc aacatcacgt tcatcactga
     2701 ccgtgatgac ccggtaatat cagggatgaa tgatattcgg ctgaaattcc ggacagccgt
     2761 acgcgtaacc gttccggtca cagacggatg gccagatgcc attgcctgta tactcagtct
     2821 gcacagactg atttctccgg gacggtaaag cagacgggta caccaggcgc acaggtcatc
     2881 atcagaaatg gaacgacacc ccggcagcgc ctccggggcg tcgggtgatt ctggtgcctg
     2941 aatcacgctt tcagggacat gctgcatcag cgcaggctct ccagcagcgt ttctgccatt
     3001 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
     3061 atacgttttc cttttgcact gcggccggaa attccgcctt tcagcatatt ctcctggatg
     3121 gtctgatatg cgctccacag gtcctgaccg taatcctccc tgcgtcgtgg tgtcagaatg
     3181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
     3241 agcgcctggc gtgccggtgg cgacaggacc agcgactgca tggcatcatg cttctcctct
     3301 atccggtcaa acacgcccac cacctcgtaa gccccttcaa tgactctgtc caccacattt
     3361 ccccggtgcg gaacacgcac ttcccccaga gactgaccac agacgcatcc gttctgacag
     3421 acgaacctga agtaacccgg cagcatctgg tagctggagg taccgtcatg ggagttgagc
     3481 agaatgattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
     3541 tgttttgtgt atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaagaatggc
     3601 tgaaagcctt cccgctgcag gctttccagt acggtgatgg tgggaatgta cgcatatcgt
     3661 ttactgcggg aagtatgccg gtcttcaccg aaaatactgg gaacatagtg catcagttct
     3721 tcgtgtgtca gcggacggtc acggcgtatc tggttcgcat aaccaaaacg ac
//
LOCUS       DAYPHN010000252         3712 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000252 DAYPHN010000000
VERSION     DAYPHN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.253"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            55..291
                     /gene="ycgZ"
                     /locus_tag="ACG0BW_004612"
     CDS             55..291
                     /gene="ycgZ"
                     /locus_tag="ACG0BW_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFK7261469.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            334..606
                     /locus_tag="ACG0BW_004613"
     CDS             334..606
                     /locus_tag="ACG0BW_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFK7261470.1"
                     /translation="MKTSDNESTKYEITGQAVLHILRMKINFSLQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGEPNRRTAIADNKSMFNGKKINRIH"
     gene            635..901
                     /gene="ariR"
                     /locus_tag="ACG0BW_004614"
                     /gene_synonym="ymgB"
     CDS             635..901
                     /gene="ariR"
                     /locus_tag="ACG0BW_004614"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFK7261471.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            1014..1262
                     /gene="ymgC"
                     /locus_tag="ACG0BW_004615"
     CDS             1014..1262
                     /gene="ymgC"
                     /locus_tag="ACG0BW_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFK7261472.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVLMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            1598..3121
                     /locus_tag="ACG0BW_004616"
     CDS             1598..3121
                     /locus_tag="ACG0BW_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFK7261473.1"
                     /translation="MRNTLIPIFIAICLFITGVTILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADEIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            3253..3471
                     /gene="ymgF"
                     /locus_tag="ACG0BW_004617"
     CDS             3253..3471
                     /gene="ymgF"
                     /locus_tag="ACG0BW_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFK7261474.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQQ"
BASE COUNT         1095 a          711 c          791 g         1115 t
ORIGIN      
        1 tttgctattg ttacctcact taacattgat taacattttt aacagaggcg tagcatgcat
       61 caaaattcag tgactttaga ttctgcggga gcaatcactc gttactttgc aaaagctaac
      121 ttgcctactc agcaggaaac tctcggggaa attgtgactg agattttgaa agatggacgt
      181 aatctgagtc gaaagtcgct ttgtgccaaa cttctatgcc gactggaaca agcgacaggg
      241 gaagaggaac agaaacatta taatgcacta attgggttgc tttttgaata atgcaacagc
      301 ctgattattt aacaggttag ctatgaagtc gttatgaaga catctgataa tgaaagtaca
      361 aaatatgaaa ttactggcca ggcggtgctc catatactgc gcatgaagat aaatttttcg
      421 ttacagactc ttattaagca attactcgta atgaaatcgg ctgaggaaaa tgctttccga
      481 cgagatttaa ttgacagcat aattcgtgat tttagcaaca gtgatacagg agagccaaac
      541 agaagaacag cgatagctga caataaaagt atgttcaatg gtaagaaaat aaacagaata
      601 cattaaaatt tcataagtaa gatgagaggt taccatgctt gaagatacta caattcataa
      661 tgcaataact gataaagcgt tatcaagcta ctttcgtagt tcaggtaatt tgttagaaga
      721 agagtcagct gtgttagggc aggctgtgac caatttaatg ctttcaggcg ataatgttaa
      781 taataaaaat attatcttaa gtctgataca ctctctggaa acaacaagtg atattctcaa
      841 agctgatgtg attagaaaaa cactggaaat cgtgttgcga tacacagctg atgatatgta
      901 acttttaatt attaagtata agtttataga aaactcagtc atcttatttt tgtctgtcgc
      961 tttagactat acacagcata attttattgg gtcaatattc ctacgaggct gacatgaata
     1021 attcaatccc cgagagattt atttttcaat gtgctttgtt taaaaatctc gagagggagg
     1081 tgctcatgac gcacgggtat gttgatagtc atattattga tcaggcttta cgcttgcgtt
     1141 taaaagatga aactagtgtg attctttctg atctctatct gcaaatcttg cagtacattg
     1201 aaatgcataa gactacacta acggatatca ttattaatga cagggaatca gtgctctctt
     1261 agaacttgag taaggttata gaataaacat cagctgtatc atcatgctgg tgcaaagtga
     1321 gcatttcagg cgttatgctt tcttaatata tccgcaatat cagggggcaa acaggaatgg
     1381 agagcgctcg ttttccattc ttgacgcctg atatcccacc taatttgttt aattctgact
     1441 acattgtgct caataattaa taggttagat gaagatggag aaaattttat cttcggcgtg
     1501 gatatttctt atgctctcta ccatgctctc tgtagatgcc agtgcgagcg aatgaatggc
     1561 attttcatgt attaaaccct ttggccggga caagcaaatg cgcaatacac tcatacccat
     1621 cttcattgcg atatgcttgt ttataaccgg tgtcactatt ttgaacatcc agctctggta
     1681 ttccgcaaaa gcagagtacc tggcgggagc aagatatgcc gccaacaata tcaatcatat
     1741 acttgaagaa gcgtcacagg cgactcaaac ggcggttaac attgccggga aggaatgcga
     1801 cctcgaggag caatatcagc ttggcactga agcagctctg aaacctcacc tgcgcacaat
     1861 catcattctc aaacagggaa cggtctggtg tacatcctta ccggggaatc gggtcctact
     1921 gtctcgtatt cctgttttcc cggacagtaa tttactgctg gctccggcaa tcgacaccgt
     1981 taatagatta cctatcctgc tctatcagag ccaatttgca gatacgcgca ttttggttac
     2041 gataagtgat cagcatattc gcggggcgct taatgtaccc ttgaaagggg taaggtatgt
     2101 attacgcgtg gcagatgaaa ttattggacc tacgggtgat gtgatgacgc ttaatggaca
     2161 ctatccctat accgagaagg ttcactccac aaaatatcat ttcactatta tctttaaccc
     2221 gccgccactc tttagcttct acagacttat cgataaaggc tttgggattt tgatatttat
     2281 tctgttaatc gcctgcgccg ctgccttcct gcttgatagg tatttcaata aaagcgcaac
     2341 gcctgaagag atcctgcgac gggctataaa taatggggag atcgtcccat tttaccaacc
     2401 tgtggtaaat ggtcgggaag ggacattgcg gggagttgag gtgttagccc gctggaaaca
     2461 acctcacggt ggatatatat cacccgcggc atttattcca cttgctgaaa aatccggatt
     2521 aatcgttccg cttacgcaaa gcctgatgaa tcaggttgcc agacagatga acgctatcgc
     2581 gagtaaactg ccggaaggtt ttcatattgg gattaatttt agcgcctcgc atattatttc
     2641 gccgacattt gtcgacgagt gcttaaatta ccgtgacagt tttacccgtc gcgatttaaa
     2701 cctcgttctg gaagttaccg agcgtgagcc attaaatgtt gatgaaagtc tggttcagcg
     2761 gttgaacatt ctgcatgaaa atggttttgt catcgcgctg gatgatttcg gtactggcta
     2821 ctcagggctt tcttatctgc atgacctgca tattgattat atcaaaattg atcacagttt
     2881 cgttggccgc gtaaacgcag acccagaatc aactcggatt ctggattgtg tattggatct
     2941 ggcgcgtaaa ctttcgatca gtatcgtcgc tgaaggtgtc gaaacgaaag aacaacttga
     3001 ctatctgaat caaaataata tcacatttca gcagggttat tatttctata aaccggtaac
     3061 atacatcgac ctggtcaaaa ttatcctttc taaaccgaag gtgaaggttg tggttgagtg
     3121 aaaatcgatc agtaaggcca tagtgcggtg taattataga cagctaatta gctcgttgcc
     3181 tcttgttact attgttcatt attttgtttg ctataattgt ttgaaagttt tgacaggatt
     3241 gccattagta gcatgaacaa tagtaataat ctggattatt tcactctcta tatcatcttt
     3301 tccattgcat ttatgctgat cactctcctg gtcatcctta ttgcaaaacc cagtaccggg
     3361 ctgggagaag tgcttgtgac gataaatttg cttaatgccc ttgtttggct ggcgatcaat
     3421 ctgattaatc gattaaggga aagactcgtc agccacaggg atcagcaata atctttcagt
     3481 ttctcactgt cagtatgcgg ctgaatgggg tgctgacagt gaacgcctgg atcattgaag
     3541 gaaaggaatt attgcgcaaa tagttgtcaa ccctggtgtt atcacagttg gctttatata
     3601 tcaccgaaat aattttcatg gcaactatta ataattttga tgtcgaagag ttatttgtta
     3661 aacaaaatcg tcaccccaaa gtgatcaatg tcatgaaaat aaggtgaaaa at
//
LOCUS       DAYPHN010000253         3675 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000253 DAYPHN010000000
VERSION     DAYPHN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.254"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..163
                     /locus_tag="ACG0BW_004618"
     CDS             <1..163
                     /locus_tag="ACG0BW_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261475.1"
                     /translation="SFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIEDEIIA
                     CATYLIPDI"
     gene            165..509
                     /gene="ymgG"
                     /locus_tag="ACG0BW_004619"
     CDS             165..509
                     /gene="ymgG"
                     /locus_tag="ACG0BW_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFK7261476.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            513..848
                     /gene="ymgD"
                     /locus_tag="ACG0BW_004620"
     CDS             513..848
                     /gene="ymgD"
                     /locus_tag="ACG0BW_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFK7261477.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(904..3566)
                     /gene="ycgH"
                     /locus_tag="ACG0BW_004621"
                     /pseudo
     CDS             complement(904..3566)
                     /gene="ycgH"
                     /locus_tag="ACG0BW_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YcgH"
BASE COUNT          995 a          849 c          751 g         1080 t
ORIGIN      
        1 ctcatttgag attattctta ttcccgtaaa aaacataatt cctatcatca cagtcacgct
       61 cattttgaat tatctgaata atagcgagcg ttctttagtt aagcagatct taattgaaga
      121 cgaaattatt gcgtgtgcaa cttacttaat accggacatc taagttgaag aaaaaaatac
      181 ttgccttcgg gcttatctca gcattgttct gctccacgcc agcgatggcg gatatgaacc
      241 gcaccacgaa aggtgcattg ctaggcgcag gcgttggttt acttactggc aatggtgtta
      301 atggcgtact gaaaggtgca gctgttggcg ctggtgttgg tgcagtaaca gaaaaaggcc
      361 gcgacggtaa aaatgcgcgt aaaggtgcaa aagtgggtgc cgccgttggt gctgtcactg
      421 gcgttctgac cggaaatggc ctcgaagggg cgattaaagg tgccgtgatt ggcggtacgg
      481 gtggtgcaat tctgggtaaa atgaaataag gaatgatgat gaaaaaattt gctttactgg
      541 cgggtctttt tgttttcgca ccaatgacct gggcgcagga ctataatatt aaaaacggtt
      601 tgccgtcaga aacctatatt acttgcgcag aagccaacga aatggcaaaa actgacagcg
      661 cacaggttgc agaaattgtt gcggtaatgg gtaatgccag cgttgccagc cgtgatttaa
      721 aaattgagca atctccggaa ctgagcgcca aagtggtaga aaaactgaat caggtttgcg
      781 ctaaagaccc gcaaatgctg ttgatcacgg caattgatga cactatgcgt gctatcggca
      841 aaaaataata tttaatatgt taccgcgtcg atgaattcga cgcggtattt tagctaacgg
      901 ggatcagaac ttatatttca ggcccaccat cattgcggtg tcattgtagc cattatcccc
      961 cagctgcacg cccacattac cccagacgct ggccgccgga ttcagattgc catttacgcc
     1021 aagtttaatt tcccccagat tacgcgcccc gtcttgttca actttcacac cgttcattga
     1081 gacagcaaaa tctttactgt tatggatcca gttcatttca acaaaaggtt caaagtcgtc
     1141 accgtgcgga ccgctgtttt tctcctgagt acgaataaag gtgcggaagc cgagtcgggt
     1201 ctggatgttg ttatttgcgt cattctcaat acgtgagcca ttggcttccg tgtgcgcatt
     1261 atgatcgacg cccatccaga tagcctgtgc ctgtggctgg aagatccacg tatatttcgc
     1321 cgcttccaga ccaaaggatt tattcagggc aaaaccgtaa cccgcttcca gcgagacggt
     1381 tgcacctttc gcgctatagg attcataggc taattcatct cctttcaccg agtttttaaa
     1441 ccagctatat tgcgcccagg cgtcaatgta tgcgcctttt ttactgatgt catcggcaaa
     1501 ccaggtggca tacagccctg cgctatagcc tctgacactc cctttcgaac gataatccga
     1561 cacgctggaa tgagttgagt tgtagtcgcg ggcataacca gccatcactc ccaaacgcca
     1621 actgtcactg tcggtaaaac caccggttaa cagatccgcc cctaactgcg agacgtagcg
     1681 atgcgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg
     1741 acgtagccaa agtcggcttg aacgctcctg ttcagttaca ggatcgatgt agcgcgtttc
     1801 acctgcacga tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac
     1861 gtaacttcca gcttcggggc gcagaacggg ggtaggcgtt tctgttgatt ctgaatttcc
     1921 ctctgagctg ttatcctcct cattcactga ctgataactt gtcaaatacc agtttttgcc
     1981 ttgttgtttt agttgatatt cgaaagctcc agctgcaatc cttgcatcac cttgtctgaa
     2041 ggcatctgct ggagattcat cgccattcac agttatgagt tcgatcccat ttagcgtggc
     2101 cgcgccgctt cctccagcat ttttcacata gacggtagtt gaacccgaag tattaccctc
     2161 aacaataagt ctgtcagtag ccgatttatc atcacctaat acggtgcgga tattaagctg
     2221 accattatta ccgacataat taccttttac ggttaacgtt tttccctggg taaatgtttg
     2281 accatcatca tacgaaagat tgataatact gtctgaatta gtcagatggg taacaacgga
     2341 gtcatctttc atattccaga ttgtgttttc tgaaagcttc agtgtagttg tgccgctaac
     2401 gagtttagtc gctccattta gagtagagtc ggacgaaatt atgtttaagc cacctccagt
     2461 attattaacc agaatattat taccaggcgt gataatactt cgattaagga tgcttacatc
     2521 cgttgtagtt ttgttaacat caatactatc caaagcactt gaaagttcga cacttgatag
     2581 tgctattttg gcttgagaat cgatatttga tgcatcaatt gatattcctt tatcttgttc
     2641 tgataccgcg gtgacattgg aaaaaaataa ctctccacca ttgtatattt ttgcaatggg
     2701 ggctttagtg ccttttgttg taatagaact atttttatat caatgttacc ttgtcgaata
     2761 gcaactgcat aacttccatg ctccccggtt tcaagtgcca cataatctaa attaatatat
     2821 gctttttccc cattagcata agcaccatag ctattagtgc cattagttgt tatggaacca
     2881 ccattcattt ttaaatcagc acggctaata acttcgtaac tgttagaatc actaacttca
     2941 tcatctacag catcgacaca tgttcgagac tctccaggac ttgaacatgc aagaacacct
     3001 actgcatttt ccccataggt aacaatgttc gttttatctg tggtttcaag acgggtgtta
     3061 ttttccacaa tcccagaatc attaacctga cttaaaagtc ccgctgaata tgcccccctc
     3121 gtctcgactg aattatcacc attcataacg atggtgccac caaattcact gactactgcg
     3181 ctggctgtta tccctccatt aacatcttgg ttatttcctt tgtcagatac gcttccagag
     3241 gtcttaatat cgctatcgct tatatataat actttgttat gactatcaac agaaatacca
     3301 taagatccat caccactagt catcacactg gcattattta tatatccagt tacaccgtca
     3361 tccactttca cagcatggct ttcgtcttta gtggttagat actctccatt acttatcgaa
     3421 ttattatctt tgatttcaat cggcgattct tcatcagtta tataggaaag tatactataa
     3481 tcatccaaca attgtgttgc atatgcatta cccaccgcca aagcaataag tcctaaacta
     3541 aacccgggga gttttttcag tttcatagat taaccatcca ttcattggat taacaaacat
     3601 tcctggtcaa ataacctatg agttcagatt accaaaacgg agggtaaaga caaaaacaaa
     3661 agcacaaaaa attta
//
LOCUS       DAYPHN010000254         3550 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000254 DAYPHN010000000
VERSION     DAYPHN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.255"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..666)
                     /locus_tag="ACG0BW_004622"
     CDS             complement(<1..666)
                     /locus_tag="ACG0BW_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFK7261478.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLNDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAIIRFTSLDQAQCFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCY"
     gene            complement(745..1050)
                     /gene="elaB"
                     /locus_tag="ACG0BW_004623"
     CDS             complement(745..1050)
                     /gene="elaB"
                     /locus_tag="ACG0BW_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFK7261479.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(1105..1566)
                     /locus_tag="ACG0BW_004624"
     CDS             complement(1105..1566)
                     /locus_tag="ACG0BW_004624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFK7261480.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMNKTLET
                     CTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            1631..2548
                     /gene="rbn"
                     /locus_tag="ACG0BW_004625"
     CDS             1631..2548
                     /gene="rbn"
                     /locus_tag="ACG0BW_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFK7261481.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTTFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWIDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            2739..>3550
                     /locus_tag="ACG0BW_004626"
     CDS             2739..>3550
                     /locus_tag="ACG0BW_004626"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFK7261482.1"
                     /translation="MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATKH
                     PLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
                     KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPLWPK
                     AVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKR"
BASE COUNT         1003 a          876 c          750 g          921 t
ORIGIN      
        1 atagcagttc agattaagcc gacgactggc agccatcatc gctgccgcat tgaccggact
       61 tgcgaaatgc aggtcagttg cccgagcaag caccactttg tcgagctcac cttcggcgat
      121 ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc
      181 tcgggtggtg gttaaatgta acccaggcaa gggtttaata ctcaccagtg tggcgataaa
      241 ttcttttgcc ttaatcgcat catgctgaag ggagctttcg ctgaatagcg tcagccgcag
      301 cgtggcttta ccaccacagc gtcgccattc aaggcgtggt aaaagtaaat taccctgcga
      361 cgggtcaaac gcattcagcc cccagatgcg taagtcggcg tgttccgggt gctgacgaag
      421 aaaacattgt gcctggtcca acgacgtaaa acggataatc gcgcccagga cagcagcttc
      481 ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac gtttgctgac tggccagcca
      541 gctcaaggca tcaaaagcgt cattgagagg gaaaggaata tcaataaccc gaatgccggg
      601 tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg tagtaagtga
      661 ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacta caaaaaaaat
      721 gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta gtaccagccc
      781 gacggccgcg cccacaccaa ttccttgcca gggtttttcg tggacgtagt catcagcacg
      841 ataaacagcc tgcttcgccc gatagtaata actgtctgaa gcctggctaa cccgtttttt
      901 cacatcatcc agcgcttttt ctgcacgcgc tttcagctca acatatttct gatcggcggg
      961 atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca ggtcgtcatc
     1021 gatacgtgta tcaccaaact gattagacat tctcgttctc catttgcgta aaacctgtca
     1081 ctaactatag acaagggtta ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg
     1141 tgggatacca tcttcttcat agacctccgt cacagggata aagccaaaac tctggtagaa
     1201 gttttgcaga tgcgcctgcg ccccgaggta aacaggttta tcaggccagt gacgcgtaca
     1261 tgtttcgagt gttttattca tcagctgctg ccccactttt tcgccacgca acgcttcact
     1321 aacaatcacc cgaccaataa cgaccggctc aagatcatca tcacttttca gaatcctcgc
     1381 atacgccacc agttcatcat ttttccaacc gagaatatgg cgattatccc ccgtcaaatc
     1441 atcaccatcg atatcctgat aagggcaatt ctgctcaaca acaaacaccg cgcaacgcag
     1501 ttgtaataag gcataaagct gggaaaccga taattcagag tgatgcagat cttgccattc
     1561 aatcataatg ctctcctttt agatgtatga cttatactag ccggaatatt ttttgaaacg
     1621 tgatgaactc atggaattaa tttttttagg tacttcagcc ggtgtgccaa cccgtacgcg
     1681 caatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggac tttggttatt
     1741 tgactgcggt gaaggcaccc agcatcagct actgcatacc acctttaacc ctggaaaact
     1801 ggacaagatt tttatcagtc accttcatgg cgatcatctc tttggattac ccggcttact
     1861 gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc cgcaaggtat
     1921 ccgtgaattt gtggagactg cgctacggat tagcggctca tggatcgatt atccgctgga
     1981 aattgtcgaa attggcgctg gcgaaattct cgatgatggc ctgcgcaaag tgaccgctta
     2041 tccgctggaa cacccactgg aatgttatgg ctatcgtatt gaagaacatg ataaaccggg
     2101 tgcattaaat gcccaggcat taaaagctgc tggcgtaccg cctggcccac tgtttcaggc
     2161 attaaaagcg ggcaaaacaa tcatgctgga agatggaagg cagattaacg gcgcagatta
     2221 cttagccgct ccagtgccag gtaaagcgct cgctattttc ggcgataccg gcccctgcga
     2281 tgccgcactt gacctggcta aaggtgtcga tgtcatggtg cacgaagcga cgctggatat
     2341 aaccatggaa gccaaagcca atagtcgcgg tcatagctct acacgccagg ctgcgacact
     2401 ggcacgtgag gctggagtcg gtaagctaat cattacccac gtcagctcgc gctatgatga
     2461 caaaggttgt cagcacctgt tacgtgaatg caggtcaatt ttcccggcga ctgaactggc
     2521 gaatgatttc accgtgttta acgtttaact ctgcttatta ttaaaaacag ggcgaaactt
     2581 gccctgttat cgcaacccgc gctacatata tccacccaca cgggcatatt gagcgatttt
     2641 tgatatttat tcatctcctt acgcactata ttcagagcaa taagttgcct ctttttatct
     2701 tcattcagca caacctttgc aactaaaagg agcctataat ggttacagtt gtcagcaatt
     2761 attgtcaatt atctcaaacc caactcagcc agacatttgc agaaaaattt actgtgaccg
     2821 atgaattact gcagtcttta aaaaaaacag cgttatccgg agatgaagaa agcattgagt
     2881 tactgcataa tattgcgtta ggttatgatg aatttgggaa aaaagctgaa gatattcttt
     2941 accatattgt tagaaaccca acaaatgaaa ccctatcgat tatccgactt attaaaaatg
     3001 cctgtttaaa attatataat ttggcacata ccgcaaccaa acaccccctc aaatcacatg
     3061 attcagatga tctcctgttc aaaaaactat tctccccttc gaaattaatg acaattatcg
     3121 gtgaggacat tcctcttata tcagaaaaac agtcgctttc aaaggtactt ttaaatgata
     3181 agaataatga actgagcgat gggacaaact tctgggataa aaatcgtcaa ttaaccacag
     3241 atgaaatagc ttgctatctt aaaaagatag ccgctaatgc aaaaaatact caagtcaatt
     3301 atcctactga tttctacctc cccaattcca acagcactta cctggaagtc gctctcaatg
     3361 ataatattaa gagcgatcca ttatggccga aagcagtcca gttattcccc ataaatactg
     3421 gcggacactg gatattagtt tcgctacaaa aaatagttaa tgaaaaaaat aacacacaac
     3481 aaataaaatg catcatattc aattcattac gtgcactagg ccatgaaaaa gaaaattcac
     3541 ttaaacgtat
//
LOCUS       DAYPHN010000255         3496 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000255 DAYPHN010000000
VERSION     DAYPHN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.256"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(330..461)
                     /locus_tag="ACG0BW_004627"
                     /pseudo
     CDS             complement(330..461)
                     /locus_tag="ACG0BW_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            743..2101
                     /locus_tag="ACG0BW_004628"
     CDS             743..2101
                     /locus_tag="ACG0BW_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFK7261483.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2340..>3496)
                     /locus_tag="ACG0BW_004629"
     CDS             complement(2340..>3496)
                     /locus_tag="ACG0BW_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFK7261484.1"
                     /translation="DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCP
                     NCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAG
                     PGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHGY
                     VMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGI
                     HPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEA
                     LEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGER
                     GALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          836 a          935 c          934 g          791 t
ORIGIN      
        1 ataaaagata atctctcaag gagtcttatc tgaaaacgaa atatatagtc cctctcgttt
       61 tgtaatatcg attggctttg ttgaatcggt tacgtacccc ggagccggtc atattttcag
      121 gtaatacaca cgctttcaga catttcggct ttcgtcattt tggtatcatg ggcatagctt
      181 ctgctacctg accatcataa tcacgcagct tcagtgaagc agtgaccacc tgtttaaccc
      241 ggtgtatcgt tatgatctta tccagcaata atggacacgc ggctaagtga accgccccgg
      301 gtttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa
      361 gcataataag ctttttctgc ttctgccggt gggatatggc cctgcctttc cagcaatcgt
      421 cgattgttat accagtccac ccacgtgagt gtggtcagct catcatactg acacgtgcat
      481 tacccctacc gcgataatgc aaccgtacaa tatagggcgt ttcctaaacg cagccctcat
      541 cactccaatc caacaagaca cagcattttt gatcacgaag aaagacaaat aatttccgta
      601 aatgtttccg ttgttacaaa taacaacagt gttttacatt tctttggcct caggcccgcg
      661 catgttgcca ttcgatgtca ttttcctgac tcgtttcttc tttatggtgt agtgtgatct
      721 tcactttatg gatgctaaca atatgaaaag aaaaattatt cccgtgctaa tcggttgtac
      781 gctctctttc tctgccctgg cggcacaacc tacggctgag cgctatgtcg tcagttttcc
      841 agaaggcacg cacgtgaact acgccggcgc atttgccagt gcattcccga acggccttcc
      901 ggttgggata ggttctggcc tgctgttcac cggtaaacag ggcgatgccc tgacattcgc
      961 aaccattacc gatcgcggtc cgaacgcgga ttcaccgaaa gaggggaaaa acgaaactaa
     1021 aattttcgtt acgccagatt tcgcgccgct gctgatgacg attcgtgtac aaaacggcaa
     1081 agcggaggcc atcgatcccc gtcctctgca tgacgataaa ggcgcgatca acggtctgcc
     1141 gctggcaagc gacgtgattg gctccaccaa tgaagtggcc ttcagcgaca cgttacatag
     1201 actgaagggg gataatcgcg ggctggatac ggaagggatc actccggatg gtaaaggagg
     1261 ttactggctg tgcgatgagt atggcccgtt cctgattaac atcgacagta aagggaaaat
     1321 cctggcaatc cacggtccgc aggcggcgga aggggaaaaa gcgattgcgg gcggtttgcc
     1381 aaatatcctc aaatggcgtc aggcgaaccg cggctttgaa ggcctcaccc gtatgccgga
     1441 cgggcgtatt attgtcgccg tgcaaagtac gctggatatc gacgctaaga gcaagaaaaa
     1501 ggcgctgttt acgcgtctgg tgagcttcga tccggcaagc ggaaaaaccg cgatgtacgg
     1561 ttatcctatc gacagcgcgg cctacagcaa aaacagcgac gctaaaatcg gagacatcgt
     1621 ggcgctcgat aaccagcaca tcctgctgat cgaacaggga agagataaaa acaacagaat
     1681 gcgtaacctg atttacgagg tcgatctcaa taaggcgagc gacctgtcag gcttcgacaa
     1741 gccgggtgag tatccggagt ttgatgatga gaaaaccctg tcacagcgcg gcattaccct
     1801 tgcgcagaaa acgcaggttg ttgatctgcg ctccttgggc tggcagcagg agaaggctga
     1861 agggttggcg ctgatcgaca gcaaaacgct ggcggtggcg aacgataacg actttggcgt
     1921 gaaggtggcg atgcaacatc cggtcgaggg caagacattc aaggattatc gggtgaacgc
     1981 ggaaggaaag ctgacgcttg acgacaagca ggttgaaacg accctcagag tgaagccact
     2041 ggagaaaccg gagtctgaca gtgaactgtg gattgtgacg ctgccggaag cgttgaaata
     2101 agtttataag gtacggacag cagtatcgct tagtttttca aattcatgta tgtgaacaat
     2161 attggggttc cggcgtagac ccccaatgaa agcgtcacca cgtgaaaaca tgcctgcacg
     2221 gtttacacgt catctcatgg tggcagccgg ttaactttcc tgctacacca gtggaaaaag
     2281 gccataagca gtccgaacaa aagtaagcgt acagcgaggg ccgtattgac ggggatgtgt
     2341 tattcagctg gcagtgctat gcgccacgga agcagttcgc tgacccggtt gaccggccag
     2401 tctgctatga cgccaggcac atggcgaagg tagctttctg gatccacgtc attcagtttg
     2461 cacgtcccga tcaggctgta cagtagcgct ccccgctcac caccatggcc agagccgaag
     2521 aacagggtct tcatcttttc acgcaaccag ctttccaggg atttcaacag cggtttcgtt
     2581 tttcgctgac gttcagcaag ccgctgctct gccggcattc cccttatatc cgcctctatg
     2641 gcgtacaact gaccgatctg ctctagggct tcttccgtca gtgctgacgg gatgcggacg
     2701 tgcacatcgt ggatctttcg gcgggcatga gcccagcagg cagcttccgt tatcccacca
     2761 ttgcgataca gctcgttgaa cccggcgtac gcatccgctt gcagcacacc gctgaagcaa
     2821 gcaagatgag tctgcggatg gatgcctttt ctgtccgggc tgtaagcgaa ccacactgca
     2881 ggtgccaacg ctgacccggc attgcggtca tcacgaacat acgcccacaa ccgcccggtc
     2941 ttcgtcttct tattacccgg cagcagtacc tggaccgggg tatcatcggc atggagtttg
     3001 ccgtcagtca tgacatagcc atgaagcgcc tcttccagcg gagacagcag ccggcagcat
     3061 gcatccaccc agcccgacag cagtgaacgg ctcagctcca caccttgccg gccgtatatt
     3121 tctgactggc aatacagcgg ggtgtgctct gcatacttcg aggtcagcac gcgggccagc
     3181 agccccggtc cggcgatacc ccgctcgatg ggccgcgaag gtgcaggtgc ctgcacgatg
     3241 gcatcgcact gagtacaggc atgtttttcc cgtaccgtcc ggataacccg gaaggcgcta
     3301 cgcatcaact ccagctgttc ggcggtatcc tcgcccagat agctcagtga accgccgcag
     3361 ttcgggcagc acggcgccgc aggcaacagt cgcttttcgt cacggggtag tgattcaggg
     3421 aacggcttac gggtgcgggt ctgacgcaac ggacgctgta ctgccgggtc atacacccta
     3481 ccagtcagcg tatcgc
//
LOCUS       DAYPHN010000256         3464 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000256 DAYPHN010000000
VERSION     DAYPHN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.258"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(114..758)
                     /locus_tag="ACG0BW_004630"
     CDS             complement(114..758)
                     /locus_tag="ACG0BW_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261485.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            960..2174
                     /locus_tag="ACG0BW_004631"
     CDS             960..2174
                     /locus_tag="ACG0BW_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFK7261486.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            complement(2754..2966)
                     /locus_tag="ACG0BW_004632"
     CDS             complement(2754..2966)
                     /locus_tag="ACG0BW_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261487.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(2966..3301)
                     /locus_tag="ACG0BW_004633"
     CDS             complement(2966..3301)
                     /locus_tag="ACG0BW_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261488.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
BASE COUNT          961 a          774 c          793 g          936 t
ORIGIN      
        1 cgcaactttg cttgtctgta tgactaaaaa taagacagga tcactgtaga acgctatgag
       61 agctattttg aggaggcgta gattctatct acgccaatat acgatctgga gggttacagc
      121 gcgtcagtgg gctttttacg cgctgcgttt atgcttatga cgccagcttt tggacgtggg
      181 tcaacaaggt taagctcttc ttcactgtgg tattcgaggt cgaactcacg ttttacatgt
      241 ctgatgtaga ttgcggtgag caggctatct ttggtcagaa agtgtccgta ggatttgcgg
      301 atcacttctt ctaccttctc gattttctca ccacccatac agagatgatt aaaccggctg
      361 tgtttccgca acatttcgtc tactgggcct gagtaaacct tgtctacttt cccaaaacgg
      421 atgattttcc ctgtgtctgc ggatacgagg caaatcagct tgtctggtga taaacgatct
      481 cgccacgtca cacctgaacg gagtgtgttg aagtaggggg catccaatcc gatgatcggt
      541 ttacgaaatg cgagtaatgg aacgtacctg atgcaactgt ttaaatgaaa attcacacca
      601 gccttatcaa gtttaattct ggtttcgtag atcgggcact tcgccgctat accgcagatg
      661 tcacaaagca acttctgctt attcaggttg gaatttgatt cgatagtgta ggagccgtct
      721 tcaagacgac gaacccagcg cgtgcgtttt agatccatat gtcatttttc tgtgattgtt
      781 agccgaggca acgataaccc accaggtgcg ccgaaggtag taaatgcctg ttttaattat
      841 tcacatatcc acaggataga tcctaataaa gagatccata gatagatcct tatatagatc
      901 aaataagatc cccgatcgct gtaagccgcg ccacgactgg tctgaagtca tgttcatgta
      961 tgctgtcagc ggtaattgat agtctgtcag cggttcaccg tatgctgcca actgtttttg
     1021 gtattctgtc agcggcaaat caagtatgct gtcagcggtt gaatgacaaa ggtgtccaca
     1081 tgtccacaaa aaataaaaaa ggtaaaaata acaaagaagt agaagataac atcgacaact
     1141 ttgaagaagg ttcccttgaa ttgtacacag gggaacttgt ccctaatagc aacaatacag
     1201 tccaaccaat tgcgttgatg cgactggggt tgttcgtccc cacgcttaag ggaacaaaat
     1261 acagtaaacg taacaagcca aatgagatcg atgcttcaaa ggagcttgtc cagctcgaag
     1321 tagcccgttc tgaaggctat tccgatatca agataaccgg tcctcgtctc gacatggatc
     1381 atgatttcaa aacgtgggtg ggcgttgtgc gttcgctggc agaatacggc gaacctaacg
     1441 gtcgtgtcga gttaagcatc acgaaatttg cgaagttttg tggctatcca tcgtcacaga
     1501 tccgcaaaac actccgcgac cggcttacaa acagtctttt gaagatcatg cgtacaactc
     1561 tgtctttcca gagaacgtat gaagaaaaga acgtcgacgg ctctaacaag atctcgcttc
     1621 tgatggtgca cctcatcaat agcgtggact acaacgagca gaaagatacg gtggtgttct
     1681 atgcagaacc gaagctggcc gaactatatc gctttgacca taaggttctt ttgcagttaa
     1741 aggttatcaa taagctacca cgcaaagaaa cagcacaggc tctgtacacc ttcatcgaaa
     1801 gtcttccaac caagcctgcg ccggtatcgc ttgctcgatt gcgtgcgcga ctcaatttga
     1861 gtagcaggaa tgtcagctcg cagaatcaga ctatacgtaa cggcttaaaa gctctccaag
     1921 atttgggcta tctcgaatac agcgagatta agcgtgggcg gtcgatctat atccagattc
     1981 acagccgcaa tccaaaactc aaagtcgcac cgccaaaacc tgaagacatc gaacccaaaa
     2041 aaccagatga gaaagctggg gaaattgatg ccaaacagaa cattatcaac aagataaccg
     2101 aactttcgca gaatttgacg cctgaaaata tcaagatgat tgagatcctt tccaatagtc
     2161 tcaaactgct ttgatatgct gtcagcggtg aaatgtatgc tatcagcggc tatttttctg
     2221 aaaagtatgc tgtgaacggt taaagatatg ctaccagcgg tatagtgaga tgaacgtatg
     2281 ctgtgagcgg taaatcatct atcaccactc aatgctctgt catacctccc cagtggttac
     2341 taacctcgaa gagtgcattc catataggtt tgcgctcact aacattctat tgctattgcg
     2401 ctgattgctt gtagtgaaag gcatttattg cctgctactc cctgatagaa cgtatgctgt
     2461 cagcggtgtg ttattgagga atttttgttt ggatatgcta tcagcggtaa ttaatggtat
     2521 atgtcagaca ttcgtatgct gccagcggta attttgttct ggttataccc ataccgggac
     2581 acattattca tgcaatttct taagtatgct gtcagcggta aaaaatcgag taaagtatgc
     2641 tgtgagcggt gagatagact ctcgatatgc tatcagcggt agttgattgt gtatgctgtc
     2701 agcggtgacg actatgaaat atgactgcgg aacgtgccgc agtcagtttg catttagtgt
     2761 attttcttta acagacccca aagtgttccg gcttttgtag tcagttttcc tgtttctgga
     2821 tcgtacatac gccattcacg ccgctggtgg acgatatacc catcttcacg ttccagacgc
     2881 tcaagaatgc caggctgctt gaaaccctta gcacgccagt agccgcttgt tttctcgatt
     2941 tccagacctg ccagtgttaa tgccattacc caacctcctt gcagtcatcg aaaaggtagc
     3001 tttgctgaga agcgtgaacg ccatagaagc ccttgtattt gttgtatacg aagtggtctt
     3061 ctccgacgcc ggttaatttg ccattacgat ttgtgagata tggggatgac aacactcgat
     3121 cgtcacgcac aacatagaac tggtcgccgc tgtctacgac catcgctcca tatggtgctt
     3181 ttatgacgtc ggtaagagcg ccattcttaa cgtcggctac agacatttca cactgataaa
     3241 cagtggtgtc ggccacaacg atcgaagaaa ggaacaacag cgcgaatggg atggccttca
     3301 tgctgccacc tctttgatta catcaaatgc tgaattaaat acgagcacga tagccaaagc
     3361 gtttatgact acgtcgttca ccggggaaaa gactttacga atgacaccgg tgaaaatgca
     3421 gtcgatgaca aaagctattg aaacgatgag taacaagtat tcaa
//
LOCUS       DAYPHN010000257         3404 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000257 DAYPHN010000000
VERSION     DAYPHN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.259"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..87
                     /locus_tag="ACG0BW_004634"
     CDS             <1..87
                     /locus_tag="ACG0BW_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFK7261489.1"
                     /translation="QGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(119..355)
                     /locus_tag="ACG0BW_004635"
     CDS             complement(119..355)
                     /locus_tag="ACG0BW_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFK7261490.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(409..1245)
                     /locus_tag="ACG0BW_004636"
     CDS             complement(409..1245)
                     /locus_tag="ACG0BW_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFK7261491.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1245..2048)
                     /locus_tag="ACG0BW_004637"
                     /note="aph(3'')-Ib"
     CDS             complement(1245..2048)
                     /locus_tag="ACG0BW_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFK7261492.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2109..2924)
                     /gene="sul2"
                     /locus_tag="ACG0BW_004638"
     CDS             complement(2109..2924)
                     /gene="sul2"
                     /locus_tag="ACG0BW_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFK7261493.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(3232..>3404)
                     /gene="repC"
                     /locus_tag="ACG0BW_004639"
     CDS             complement(3232..>3404)
                     /gene="repC"
                     /locus_tag="ACG0BW_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFK7261494.1"
                     /translation="IDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAA
                     GKYDITRPKAAG"
BASE COUNT          780 a          979 c          966 g          679 t
ORIGIN      
        1 cagggactcg cggtgattgt cgatggattg gcgaaaagga ggctcgttgt caggaacgtt
       61 gaaggaccga gaaagggtga cgattgatca ggaccgctgc cggagcgcaa cccactcact
      121 acagcagagc catgtagaca acatcccctc cccctttcca ccgcgtcaga cgcccgtagc
      181 agcccgctac gggctttttc atgccctgcc ctagcgtcca agcctcacgg ccgcgctcgg
      241 cctctctggc ggccttctgg cgctcctgct gcggcgtccg ctcgtgggcc gtggcgcggg
      301 tcggggtcgt ttgcgggaag gggcggaatc ctacgctaag gctttggcca gcgatattct
      361 ccggtgagat tgatgtgttc ccaggggata ggagaagtcg cttgatatct agtatgacgt
      421 ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg cgttgctcct cttctccatc
      481 cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc tggtcgagca ggcgacgcgg
      541 atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt gcaatgcgtc taggatcgag
      601 acaaaggtcg tctctgtcag ccggatcgta gaacatattg gcggcgccaa agcccacttc
      661 accgaccaga ccgacgggat ctatcaccag ccagccgcga ctggagaaca tgatgttttc
      721 atgatgcaga tcgccatgta gcccacgcag ttccgaggca ttgctcatca tttgatcggc
      781 tataatcgcc gcgtggacgt agtcagtttg acaacctgcg ttttgatcat cgcgcgcccg
      841 ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg gcagaaggca ggggttcctc
      901 agatgcggca tacagcttcg ccattagttc cgctgcaatt tcggtcgcct ggtagtcgcc
      961 gtgctcggca acgatgtgag agagcattcg ctccccggca tattcgagca acatcagatt
     1021 gttctcacga ccgagcaacc ggactgctcc cctcccattg cgccatacca gatagtcggc
     1081 cccgcgcagt tcatcagcaa tgtcttttat aggtttcaat cccttgacga ttgcaggagt
     1141 cccgtctggc aatgaaactt tccaaacgag gctggaaaag gtgtccgcaa tgagaacagg
     1201 ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg aacatcaacc ccaagtcaga
     1261 gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg gggcttcgat ccccaataca
     1321 ttgaatagga cagcgaaggc gcgctctgct tcatctggcg ctgcccagtt ctcttcggcg
     1381 ttagcaatca tgagtgccaa atcggcatag cgatctgctg ttccgagccg cccaaggtcg
     1441 atcagacccg tgcattgaag agttttaggg tccaccatga agttcggcat gcagggatca
     1501 ccatggcaaa caaccatatc ggtgcgctct tggtcgagcc gcaccggtag ctctcgttcg
     1561 acacgagcca aaagatcgag ctgcggcgta ctcttgtcct cgtccggtaa gaagtcggga
     1621 ttgacggcat tgcgggacac cacatcaacg gcgcgtccga acattcgcga cagcctgcgc
     1681 tcaaacggac attgatcaac cgataggctg tgaacagcgc caagttgctg ccccattgac
     1741 ggccacgctt tgagcaaatc cgctccagac agatcagccg ccggtactcc cggaattgcc
     1801 gttatcacca agcatgcacc ctcctgttcc tcctgccagt tgatgacctc ggggcaagcc
     1861 acacctcgac ctttgagcca aatgaggcgg tcacgctctc cagcgagctc accgcggcgg
     1921 gaagcaggtg cgattttcgc gaaggcatgc ccgtcaccac gtcgaaaaac aaaatcacca
     1981 gattctccgc ctctgacagg caaccagtca gaatgcgatt caccaaaaaa aatattagtt
     2041 cgattcaatg gaggttcctt cagttttctg atgaagcgcg aatatagaga aatatcccga
     2101 atgtgcagtt aacgaattct tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg
     2161 cgcaaggggc gcggctcgtg tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc
     2221 tctgcagcga gtgtcgcggc cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga
     2281 aaggatttgc gcgaaacaga cagaagcacc ggcaaatcga agcgcagccg caattcatcg
     2341 aaccgcgcca gcaccgagag cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg
     2401 ggatcaagga caaggcggtt gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg
     2461 tcaaagaacg ccgcaatgtg atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc
     2521 tgcccgtctt gcaccgaatg cataacgacg agtttggcag atgatttcgc caattgcgga
     2581 tagaacgcag cgtctggaaa accgcgaata tcattgagat aggccacacc acgcgacaag
     2641 gcataggctt gcgtcgcggg ttgataactg tcgagcgaga cgggaatgcc atctgccttg
     2701 agcgcgtcca gcaccggcgc gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg
     2761 tcgggattgc tggatgccgg accgaggtcg atcacatctg ccccctcggc catcagctta
     2821 cgcgcctgcg caatggctgc gtctggcgcc agataccggc ctccatcgga gaaactgtcc
     2881 gaggttatgt tgacgatgcc gaaaatgatg agcgatttat tcatgggggc ttctataata
     2941 ataataatcg agcatgagta tcatacggat gctcgggtcg aaagggaatc cccaggcgag
     3001 taacctgttt gcggtgatcc attagctgca ggagcagaag agcatacatc tggaagcaaa
     3061 gccaggaaag cggcctatgg agctgtgcgg cagcgctcag taggcaattt ttcaaaatat
     3121 tgttaagcct tttctgagca tggtattttt catggtatta ccaattagca ggaaaataag
     3181 ccattgaata taaaagataa aaatgtcttg tttacaatag agtggggggg gtcagcctgc
     3241 cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg tccagcccag
     3301 cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc
     3361 actggcctct gacggccaga catagccgca caaggtatct atgg
//
LOCUS       DAYPHN010000258         3383 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000258 DAYPHN010000000
VERSION     DAYPHN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.260"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..546)
                     /locus_tag="ACG0BW_004640"
     CDS             complement(<1..546)
                     /locus_tag="ACG0BW_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HFK7261495.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLP"
     gene            complement(636..1451)
                     /gene="allR"
                     /locus_tag="ACG0BW_004641"
     CDS             complement(636..1451)
                     /gene="allR"
                     /locus_tag="ACG0BW_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFK7261496.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELSYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(1529..2011)
                     /gene="allA"
                     /locus_tag="ACG0BW_004642"
     CDS             complement(1529..2011)
                     /gene="allA"
                     /locus_tag="ACG0BW_004642"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFK7261497.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            2241..3167
                     /gene="allS"
                     /locus_tag="ACG0BW_004643"
     CDS             2241..3167
                     /gene="allS"
                     /locus_tag="ACG0BW_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFK7261498.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YTPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            3236..>3383
                     /locus_tag="ACG0BW_004644"
     CDS             3236..>3383
                     /locus_tag="ACG0BW_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="catalyzes the selenophosphate-dependent transfer of
                     selenium from selenophosphate for conversion of
                     2-thiouridine to 2-selenouridine at the wobble position in
                     tRNA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFK7261499.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERA"
BASE COUNT          873 a          909 c          813 g          788 t
ORIGIN      
        1 cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc gcaacctgta cgtcaaacgg
       61 taaatccacc agtaccggcc ccggacgacc agaacgcatc aggtgaaatg cctgttgcag
      121 cacacgaggc accagcgccg cttcacgaac tgtaaccgcc attttgctga ccggtttagc
      181 aattgcttca atatctacgg cctgaaaatc ttctttatgc agacgggcgc gcggtgcctg
      241 accggtaata cacagaatag gaatggaatc agcagaagcg gaatagagcg cggtgatcat
      301 gtcagtgcct gctggtcccg aggttcccag acatacgccg atattccctg ccgttgcgcg
      361 ggtataacct tccgccatgt gcgaagcacc ttccacatga cgcgccagaa tgtgacgaat
      421 accgccgtgc ttacgcatcg ctgagtagaa cggattgatt gcagctcccg gaacgccgaa
      481 ggcggtggtg ataccttctt tctccagcac atacattgcc gcgtcaacgg ctctcatttt
      541 tgccatttta ttcctacctc tattaattgg aaaaattttc caactttcaa attttgtcaa
      601 aaaaacccgc tccgcagagc gggatgcgac agacactatg gatgtgcttt cagtcccaac
      661 gccgtgctga tatcgcgggc ggtgtctctg accagctcac cctgactgac aaaacgatct
      721 tctgtcagtc ttgatgaagg cccggagatg gagatagcgg caacaacgct acctacatca
      781 tcgtaaattg ctgaagctat acaattcagg ccgacaacat gctcttcttt atctacggta
      841 tagctcagtt cacgcgcttg ttccaggtcc ttcagcaagg tgggcatatc cacaagcgta
      901 gttggcgtaa actgctgcaa accggtttgc agaatgatgc tcatcaactc ctcttccgcc
      961 agcggataaa gcagcgcttt gcccgcaccg gaagcatgca gcggcagacg actgcccagt
     1021 ggcgcgcaca tcctgaccat cgatttacac tctaactgac caattaatac cgcttcatta
     1081 ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg gcgcataaac
     1141 ggcccggcga cggagaggac atcgcggtta tggatgtacg ccgcaccgac gttaaagaca
     1201 cctaatccta tatgccacca gcctaactga ctgtcctgat agacaaaatc cgctgcctgt
     1261 aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag agagatatcg
     1321 ctaaccgacg aacttccccc acttttttcc agatattgca gaatcgcaat tccccgctct
     1381 aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta
     1441 acttccgtca tacctacgtt aactccgacc tacaagtgag cataaacaat gaacaattag
     1501 tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt tcaggaatac
     1561 tttcaacatc acagttgtca ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct
     1621 gccaggcgaa aagtggatga tgccagacgt tacgatggta attcaccccc tgttcgccgt
     1681 tggtaataaa cgcccgcagc gttgacaggt ctggtttgtc gtcacctaac gccacgacca
     1741 ccacaaacac ttcacctttc atcgggataa aggcctgagt acccagcgga tgacgttcga
     1801 gttcgtgaat ggtcagcggc agattcgccg gttgcgcgcg gttaatgctg ataagcgtac
     1861 ggtcttgctc aagaatctca accagcgcca aatcgtggta acgctccacc agtccattgt
     1921 taatatggaa aaaatcccgt ttctgcgttt cgattacgtc gccataagca ctaaaggctt
     1981 cctgacttaa cggtaatacc tgaagtttca tctttttgtc cttataacat aattccgttc
     2041 aaccaccgcc gaccggtcag cttggtttca ataacaggca atctaaaacc ttaagagaac
     2101 aacatcaaca caaaagttgg aatatttttc caaaactaga tttagtccac aaattgaaca
     2161 gggttatcat gtaaaaatac tgataaatca gaggataaga aaatcacgca acttgcggat
     2221 ttaaaagggg atacctgaag atgttcgatc cagaaacctt gcggactttc attgcggttg
     2281 ctgaaacagg aagtttttca aaagcggcag aacgattgtg taaaaccacg gcgacgatca
     2341 gttatcgcat taaacttctg gaagagaata ccggagtggc gctgtttttc cgcacgactc
     2401 gcagcgtgac gttgacagcg gctggcgagc atctactttc ccaggccaga gactggctga
     2461 gctggctgga aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg gaacgccagg
     2521 tgaatattgt catcaacaac ctgctctaca ccccccaggc cgtcgcccag ctgctggcgt
     2581 ggctgaatga acgttacccg tttacccagt ttcacatctc ccgacaaatc tatatgggcg
     2641 tctgggactc gctattgtac gaaggttttt cactggctat cggcgtcacg ggaaccgagg
     2701 cgctggcaaa tacctttagt cttgatccct taggatcggt gcaatggcgc tttgtcatgg
     2761 cggcggatca tccgctggcg aacgttgaag agccgctaac agaagcacag ttgcggcgct
     2821 ttccggcggt caatattgaa gacagcgccc gaacactaac caaacgcgtc gcctggcgat
     2881 tgccggggca aaaagagatt attgttcccg atatggaaac gaaaatcgcc gcccatctgg
     2941 cgggcgttgg cattggtttt ttgccaaaat cactttgcca gtcaatgatc gataatcaac
     3001 aactggtcag ccgggtaatc ccaacgatgc gccctccttc gccattgagt ctggcatggc
     3061 gcaaatttgg cagcggcaaa gcggtagaag atattgtgac cttgtttacc cagcgcaggc
     3121 cggaaatcag cggattttta gaaattttcg gcaacccacg cagttaaaaa tcaagtttcc
     3181 ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt taactatgca
     3241 agagagacac acggaacagg actatcgtgc cctgctgatt gctgatacgc ccattattga
     3301 tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc gccgctatca acctgccgtt
     3361 aatgaataac gatgaacgtg ccg
//
LOCUS       DAYPHN010000259         3381 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000259 DAYPHN010000000
VERSION     DAYPHN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.261"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            315..593
                     /locus_tag="ACG0BW_004645"
     CDS             315..593
                     /locus_tag="ACG0BW_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261500.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSHTFQQRIWR"
     gene            595..1644
                     /locus_tag="ACG0BW_004646"
     CDS             595..1644
                     /locus_tag="ACG0BW_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFK7261501.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQEQHPLQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            1657..2031
                     /locus_tag="ACG0BW_004647"
     CDS             1657..2031
                     /locus_tag="ACG0BW_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFK7261502.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            2028..2849
                     /locus_tag="ACG0BW_004648"
     CDS             2028..2849
                     /locus_tag="ACG0BW_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFK7261503.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            2993..3068
                     /locus_tag="ACG0BW_004649"
     tRNA            2993..3068
                     /locus_tag="ACG0BW_004649"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3026..3028,aa:Ile,seq:cat)
     gene            3169..3245
                     /locus_tag="ACG0BW_004650"
     tRNA            3169..3245
                     /locus_tag="ACG0BW_004650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3203..3205,aa:Arg,seq:tct)
BASE COUNT          858 a          768 c          907 g          848 t
ORIGIN      
        1 gtatcacaga taagaaagga gcgtaatgtg cagatttgtt gttttccata tttactcact
       61 ttaacataat caatattgat atggttgttg tttcggtggt ttcaaacgag atgttatggt
      121 gatctggtaa aattgcataa cattaaaatt taatttatct aatcgctttt aataataagc
      181 gttgtgttta tcccaacaat ctgttgtttg acttttattc cattaatgta ggggctttac
      241 actggaacca gtttatttat actttatacg ccagcctgaa caactggcac ctgctgcgcc
      301 agcagagaca accgatggcg cacgatacca aattacacaa ttctgatgat tctgccgtct
      361 ttgccagcag gtgcggacgg cgttttcatg cattcaaatc ggactggttc cagcatccac
      421 catgcactga agagcaggct gaatggataa ttcagtgtta ccgcaggcgc ggatacgagg
      481 ttaaaaaagc ccttagcctc gactaccgtc actggataat ctcagtcaga ttgccttact
      541 ccgaacgccc accgcgtccg tcccacacat tccagcaacg catctggagg taacgtgcgg
      601 gtattacttc gacctgttct ggtaccggaa ctcgggctgg tgatcgttaa gccgggccgt
      661 gaatccatgc cggtattcca caatacccgg ttactggtgg agccggaacc gaaaagcatg
      721 cgtaatctgc cgtccggggt cgttcctgcc gctcgccagc cgctggtgga agacaaaaca
      781 ttgctgccgt ttttcagtaa cgcacgggtg attcgtgctg ctggtggtgc tggtgcattg
      841 tctgactggc tgttgcgcca tattaaatcc tgccagtggc cacacggcga ttatcatcac
      901 agcgaaaccg tcattcaccg ttatggtacc ggcgcaatgg tgttgtgctg gcactgcgac
      961 aaccaactgc gtgaccagac atccgaatca ctcgagcaac ttgctcatca aaacctgtca
     1021 gcatggatga ttgacgtcat cggtcacgca ataagcggta cgcaggagcg tgaattatct
     1081 ctggctgaat tatcctggtg ggcggtccgc aatcaggtgg cggacgcgct accggaagcg
     1141 gtattacgtc gttcgctggg gttgcgtgcg gaaaaaatcc gctcaatgta ccgtgaaagc
     1201 gacatcgtac cgggagagca gaccgccacc agcatactga aacagcgcac aaaaaatctt
     1261 gcactgttgc ctcacgccca ccaggaacaa cacccactac aggaaaagac ggtggtaagc
     1321 attgccgttg atcctgagtc tccggaatct tttatgaaac gacctaaacg tcgccgctgg
     1381 gttaacgaga aatacacacg ctgggtgaag acacagccgt gtgcgtgttg tggtaagcca
     1441 gccgacgatc cccatcacct gattggtcat ggtcagggcg gaatggggac aaaatctcac
     1501 gatattttca cgctaccgct gtgtcgggag catcacaacg agcttcatgc ggatccgctg
     1561 gcgttcgaag aaaagcatgg ttctcaggtt gatttaatct ttcgttttct tgatcacgcc
     1621 tttgcaactg gcgtgcttgg gtaaaagagg tgactgatgc tcatagattt ggttttacct
     1681 tacccgccga cggtgaacac ttactggcga cgccgtggca gcacatattt tatctcggag
     1741 gagggaaagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
     1801 agcctgtccg gaaggctggc gataaaggtg attgcagagc caccggataa gcgtcgtcgc
     1861 gacctggaca atatcctgaa agcaccgctg gatgcgctga cgcatgcggg agtgttaatg
     1921 gacgatgagc agtttgatga aatcaatatc gttcgtggtc agccagtatc tggtggacgt
     1981 ctgggggtga agatttaccc cataatgcat gaagagcagg tcaaaaaatg aaactggaag
     2041 atttaccgaa atactactcc ccaaaatccc cttgcctgac cgatgcatcg gcctcaacgt
     2101 caaaagatgc gctgagtatc actgatgtga tggccgcgca gggcatgaca cagaatcggg
     2161 ctgagatggg gttttctgcg ttcctgggga aaatgggcat cagtatgaat gacagggcgc
     2221 gggcaacaga attactggca gattatgcac tcagtcggtg cgatcgtgtg gcggcgttga
     2281 gaaaacttcc ggcagaaata aaaccggtag tgatgcgcat tatggcttcg tacgcttttg
     2341 aggattatgc ccgcagcgca gcgagtaaaa agcagtgccc ttgttgctat ggggaaaaat
     2401 ttattgaaag cgtagttttt acaaacaagg tccagtatcc ggatggtaag ccgccggtat
     2461 gggcaaagtg tacgaaaggt gtgtatccgt cttactggga agaatggaaa aaagtcaggg
     2521 aggtggtaaa agttgcctgt ccggagtgtg gcggaaaggg tgaggtttcc accgcctgta
     2581 aggattgccg tgggcgtggt gtcgccattc accgtgaaga gtcggtaaaa cgtggtatgc
     2641 ctgttatcag agactgccag cgttgtggtg gtcgtggcta tgaaagacta ccatcaacgg
     2701 aggcatttaa tgctatatgc gaggtgacaa accagataac acgcgcgtca tgggaaaaaa
     2761 cagttaagaa attctatgat gcgctggtga cccggtttga tattgaagaa gcatgggctg
     2821 agcggcagtt aaaaaaggta actaggtaac aaggttgatt tttccggaat ctgtggtaaa
     2881 ttcgtcataa tgatgggctt tttatgcctg acgttagaag agtttctaca acccgccgcc
     2941 gagcgggttt tttattgcgg aattaattac ggaccgttat tattctgctc acggcccttt
     3001 agctcagtgg tgagagcgag cgactcataa tcgccaggtc gctggttcaa atccagcaag
     3061 ggccaccatc acaaaccgcc attagcttat caggaagagc agacgacgcg ataacagggt
     3121 tgttggtgcg ggggcgggtc cccgatggcg gtccattatc ggtattcagc gttgttagct
     3181 cagccggaca gagcaattgc cttctaagca atcggtcact agttcgaatc cagtacagcg
     3241 cgccatattc attcttccag attccttccg gcagagcctt atactgaaat atacttggct
     3301 caggatcttg ttgaaaatat tatatgtttg tcaaaaataa aagttctgtt aagtattgat
     3361 tgagtgtttg ttataccgtc t
//
LOCUS       DAYPHN010000260         3323 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000260 DAYPHN010000000
VERSION     DAYPHN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.262"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            187..1707
                     /locus_tag="ACG0BW_004651"
     CDS             187..1707
                     /locus_tag="ACG0BW_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFK7261504.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1733..2071)
                     /gene="maoP"
                     /locus_tag="ACG0BW_004652"
     CDS             complement(1733..2071)
                     /gene="maoP"
                     /locus_tag="ACG0BW_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFK7261505.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2190..3029
                     /gene="hdfR"
                     /locus_tag="ACG0BW_004653"
     CDS             2190..3029
                     /gene="hdfR"
                     /locus_tag="ACG0BW_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFK7261506.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYKNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3124..3199)
                     /locus_tag="ACG0BW_004654"
     tRNA            complement(3124..3199)
                     /locus_tag="ACG0BW_004654"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3164..3166),aa:Trp,seq:cca)
     gene            complement(3208..3284)
                     /locus_tag="ACG0BW_004655"
     tRNA            complement(3208..3284)
                     /locus_tag="ACG0BW_004655"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3248..3250),aa:Asp,seq:gtc)
BASE COUNT          877 a          823 c          826 g          797 t
ORIGIN      
        1 gtatgaatgt tgttcccttc ctcgtagttc atcctttgcc cgctgcattt ttgcgagcat
       61 cattccaccg ccagaacaac tcaacttcac ttgcacaaat aaacaggaat cagccactaa
      121 aattcacgaa ttagcgggct acagcagcga tatcttatgc acataatggt gactcaagga
      181 gggcttatgt cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa tgcgccccca
      241 atcactgttg aggtacatat cagtaaaggt ctacccggct taacgatggt gggcttacca
      301 gaaacaacgg taaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa tagcggatat
      361 gaatatccgg cgaaaaaaat caccatcaac cttgctccag ccgatctgcc aaaagaaggg
      421 ggacgatatg atttgcctat cgctattgcg ttgctggcgg cctcagaaca gcttacagcc
      481 aataagttag atgaatatga attagtcgga gaactggcgc ttacaggcgc tctgcgtggc
      541 gttcccggcg caatctccag tgcaactgaa gccattaagt cgggcagaaa aattatcgtc
      601 gcgaaagata acgaggatga agtggggcta attaacggtg aaggatgcct gatagccgat
      661 catctgcagg ctgtctgtgc gtttctggag ggtaagcacg ctctcgaacg cccgaaacca
      721 actgatgcag tatcccgggc gctacaacat gatctcagtg atgttgtcgg tcaggagcaa
      781 ggaaagcgag ggctggaaat taccgccgct ggcgggcaca accttttact gattgggccg
      841 ccgggaacag gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc agatttaagc
      901 aatgaagagg cactggagag cgctgcgata ttaagtctgg taaatgctga atcagtacaa
      961 aaacaatggc ggcagcgccc gttccgctca cctcatcaca gtgcgtcgtt aactgcgatg
     1021 gtgggcggtg gtgcaattcc agggccaggt gaaatttcgc tggcgcataa cggcgtgctt
     1081 tttcttgatg agctacctga atttgaacgg cgtacactgg atgccttgcg agagccgatt
     1141 gaatccgggc agatccatct ttcacgcaca cgagcaaaaa taacctatcc agcccgtttc
     1201 cagcttgtcg cggcgatgaa tcccagccct accggacatt atcagggaaa ccataaccgc
     1261 tgcacaccag aacagacatt acgttatctc aaccggctct cggggccctt tctcgaccgc
     1321 ttcgatctct cactggagat cccattaccg ccccccggca ttttgagtaa aacggtagtg
     1381 ccgggagaaa gcagcaccac cgttaaacaa cgtgtaatgg ccgccagaga gcgccaattt
     1441 aagcggcaga ataagttgaa cgcctggctg gatagtccgg aaatacgcca attctgcaag
     1501 cttgagagcg aagatgcgca gtggctggaa gaaacgctga tccatctggg gttatcgatt
     1561 cgtgcctggc agcggttatt gaaagttgca cgaacaattg ctgatattga tcagtctgac
     1621 attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc
     1681 atccatctgc aaaaactact gacataaaaa aaagggcatt tcgccctttt tattaatcgt
     1741 cagaatcggt gtagtcttca gcaccttcaa cctgcggttt gccgccggaa agggtgtgaa
     1801 aacgttttgg acgcttgata cgcgtcatat acttggacca cacgcgttct gcttctgtca
     1861 ctggctcacg ttcgccacgg catactgcta cgaagagttt ctcttcctcg gtaaccggct
     1921 cgcgtttgcc aagatccaac tcattgaagg cataaccatg acgctcaagc agttgtgcct
     1981 ctttgatggt gaagtcacca tgacgagaga atccacgtgg ataatgttta ttgtcgaaat
     2041 atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct ttgaccgagc
     2101 tagttatggc gcggagtatt agttacgctt gacagagtgt aaaacaaaac atttaaatca
     2161 taacgacaaa taattttgcg gagagcactg tggatacgga attgttaaaa actttcctgg
     2221 aagttagccg aacgcgtcac tttggtcgag cggctgaatc gctctatctg acccagtcag
     2281 cagtgagctt tcgaatcaga caactggaaa atcaactggg tgtgaatctt ttcacccgcc
     2341 acagaaacaa tatccgttta accgctgctg gtgaaaaact actgccttat gcagaaacgc
     2401 tcatgagcac ctggcaggcc gcccgtaagg aggtggcgca tacctcacga cataacgagt
     2461 tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct taatcagtgg ctgggacgct
     2521 tgtataaaaa tcaggatgcc catacaggct tacagttcga agcgcgaatt gcccaacggc
     2581 agtctctggt aaaacagctg catgaacgcc agcttgatct tcttattact actgaagcgc
     2641 ccaaaatgga cgaatttagc agtcagttgc tcgggtattt cactttagcg ctttatacca
     2701 gtgccccttc caaactaaag ggagatctta actatctgcg actggaatgg gggccagatt
     2761 ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt tcccattctg acaaccagtt
     2821 ctgctgaact ggcacagcaa cagattgcga cgcttaatgg ttgcacctgg ctacccgtca
     2881 gttgggcgcg taaaaagggc ggcctgcata ccgttgtcga tagcacaaca ctttcacggc
     2941 cgctttatgc catatggctg caaaatagcg ataaaaatgc gttgattcgc gatctattga
     3001 aaattaacgt gctggatgaa gtgtattaat ctgaatggct ggcaaggatg ccggtagaag
     3061 gatttacttc ggagaggtta tttcagataa aaaaaatcct tagctttcgc taaggatgat
     3121 ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta
     3181 ccaattgaac tacgccccta attagggtgg cggaacggac gggactcgaa cccgcgaccc
     3241 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
     3301 accaccggtt ttatgaccgg cta
//
LOCUS       DAYPHN010000261         3315 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000261 DAYPHN010000000
VERSION     DAYPHN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.263"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            131..1342
                     /gene="bluF"
                     /locus_tag="ACG0BW_004656"
     CDS             131..1342
                     /gene="bluF"
                     /locus_tag="ACG0BW_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFK7261507.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELINN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            1547..2278
                     /gene="bluR"
                     /locus_tag="ACG0BW_004657"
     CDS             1547..2278
                     /gene="bluR"
                     /locus_tag="ACG0BW_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFK7261508.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIEAPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2499..2903
                     /locus_tag="ACG0BW_004658"
     CDS             2499..2903
                     /locus_tag="ACG0BW_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFK7261509.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2956..3066
                     /locus_tag="ACG0BW_004659"
     CDS             2956..3066
                     /locus_tag="ACG0BW_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261510.1"
                     /translation="MEIKVQRLSLWMINTVFLLPLINNHQANTIKLIFEM"
BASE COUNT          986 a          687 c          692 g          950 t
ORIGIN      
        1 actcatcttc aactttttcc attttttatc aatgtaaaga aacagttatg ttttggtatt
       61 aaaagaaaga caccggaaga tatgtctgtt accgtcttac tctcgcctct cccattaccc
      121 tggacagtta atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt
      181 caaaaaaatc gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac
      241 agggatctta ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca
      301 ggttaaaatg atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct
      361 gctgtgcgat tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt
      421 gcgcctgcac gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt
      481 tcagctaact tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga
      541 acaagcaacc tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc
      601 tgataaagaa cttgattcct gtaccctttc accaactata aacgaccact ttgcctttca
      661 tcctattgtc gatcccttat cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa
      721 tgaagacagc ccgtcagcta tagcggttgg gcagcgtaaa gacggggaaa tctacaaagc
      781 ggatctcaaa agtaaggcgc ttgcattcgc gatggcacac gcacttgacc tcggtgataa
      841 aatgatttca atcaatctat tacctatgac cctggttaac gaacctgacg cagtctcttt
      901 tttacttgat gaaataaagg ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac
      961 tgaaagtgaa gtcatatctc ggtttgatga gtttgcagaa gcgattaaat cgcttaaggc
     1021 tgccggtatc agtgtagcaa ttgatcattt tggcgcaggt tttgccggtt tgttactcct
     1081 gtcacgcttc cagcctgaca gaattaaaat cagtcaggaa ttgattaaca atgttcataa
     1141 aagcgggcca cggcaggcaa ttattcaggc gatcataaaa tgctgtacat cacttgaaat
     1201 tcaagtctct gctatgggtg tgacaacacc agaagagtgg atgtggcttg aatctgcagg
     1261 aattgagatg tttcagggag atctgtttgc gaaagctaaa ttgaatggta tcccttcagt
     1321 tgcgtggccg gagaaaaaat aattttcagc acattatttc gtatgatttc tgaaaatcat
     1381 cgtgaaaatg aatacgttat gtttaaattt aatccacatt aaaaataatg tttcattcaa
     1441 tttagctgaa atcttattta tactttgtac aattttagcg tatattgtac aaagtattat
     1501 tgggtcgtgt acaggcgacg gagatttatg accgcaagga ggaagtgtgg cttattacag
     1561 cattggtgat gttgccgaac gttgcgggat taatcctgtc actctccggg cctggcaacg
     1621 ccgctacggt ttgttaaaac cacagcgcag tgaaggcgga caccgactct ttgatgaaga
     1681 agacatacaa cgcatcgaag agatcaagcg ttggataagt aatggcgtcc ctgtaggcaa
     1741 agttaaagca ttactggaaa ccaccagcca ggatacggaa gatgactgga gccgcctgca
     1801 agaagagatg atgtcaattc ttcgtatggc taatcctgcc aaactacgcg cgagaattat
     1861 ttcactgggt cgagagtacc cagtagatca attgattaat catgtttacc ttcctgttcg
     1921 ccagcgtctc gtgcttgatc acaacacctc ccgcattatg agcagtatgt ttgacggcgc
     1981 attaattgaa tacgcagcag cctcgctttt cgaaatgcgc cgtaagcccg gtaaagaagc
     2041 cattctgatg gcgtggaatg ttgaagagag agcacgattg tggctggaag catggcgttt
     2101 atcattgtca ggatggcaca tttctgttct tgctgatccc attgaagcgc cgcgcccgga
     2161 actgttccca acgcaaacat tgattgtctg gacaggcatg gcaccaacga gaaggcaaaa
     2221 cgaactttta caacactggg gtgagcaagg ttataaagtc atctttcatg ccccctaatt
     2281 tcacctttat cccgcccctt agacagaatc ttataatctg aacccacaat agcgtttgta
     2341 ccggagctgg tccgttgcaa aatgctcacc gggagaattc aggtaatccc cctttcacac
     2401 tttgtacata tcacagccaa aggacagacc agattgaagt ggtaaaaccg atcgtgcaca
     2461 ctacgcagtc tcacaactaa caagaggtta ttatcctgat ggatcaggtt gttgtttttc
     2521 aaaagatgtt tgagcaagtg agaaaagagc aaaacttctc ctggttttat tcagaattaa
     2581 aacatcaccg cattgcacat tacatttatt atctggctac ggataatatc agaattatta
     2641 ctcacgatga cacggttttg ttattaagag gaaccaggaa cctgttaaaa gttagtacga
     2701 ccaagaaccc tgctaaaata aaagaggccg cattgcttca tatttgcgga aaatcaacat
     2761 ttcgggaata ctgttcaaca ctggcaggcg cgggcgtttt ccggtgggtt actgatgtta
     2821 atcataacaa acgcagttac tacgccattg ataatacgct tttatatatt gaagatgtag
     2881 aaaacaataa accattaatc tagctaaagt tggaagctta agaaatgctt cataattcag
     2941 taagacatta gcataatgga aataaaagtg cagagactat ccctatggat gataaatact
     3001 gtctttttat tgccactcat aaataatcac caggccaata ctatcaaatt gatatttgaa
     3061 atgtgattac ttgactttct atacgttatt ttataacggt tagcaaattt ataaaaccaa
     3121 cgggcgtgcc atacgcccgt ttcaatactt aacgcacatg tgttttggtt tagtcatcat
     3181 ccggttatat gtattttagc caggaatggg ttaaaccatt cctatataac taaaaaattg
     3241 aaaccttatt ctcatgtcat gcttatattc gtcattatcg ttatataaaa aggccaacca
     3301 taatgtttag caaat
//
LOCUS       DAYPHN010000262         3198 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000262 DAYPHN010000000
VERSION     DAYPHN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.264"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            156..1838
                     /gene="ydeN"
                     /locus_tag="ACG0BW_004660"
     CDS             156..1838
                     /gene="ydeN"
                     /locus_tag="ACG0BW_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFK7261511.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKESFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1890..3047
                     /gene="ydeM"
                     /locus_tag="ACG0BW_004661"
     CDS             1890..3047
                     /gene="ydeM"
                     /locus_tag="ACG0BW_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFK7261512.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3013..3135)
                     /locus_tag="ACG0BW_004662"
     CDS             complement(3013..3135)
                     /locus_tag="ACG0BW_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261513.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          965 a          688 c          649 g          896 t
ORIGIN      
        1 gtagaataga aagtaatgtc aacgcttcat ggactgttat ttcccttttt ttagctaaat
       61 ctgctatctc tttatgtgac taacttcact tacattcact tatttctctt cgtaaaatta
      121 ctttggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
      181 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
      241 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
      301 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actgaccatg gatgatcttg
      361 gttatggaca acttcctttt gataaggaat cttttgaccc aaaaacaatg gaaaatcgtg
      421 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgcg caaaaatcaa
      481 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcgc
      541 acggtgtttc cggcccctcc cgcgccgcaa taatgaccgg tcgagcgccc gcccgctttg
      601 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
      661 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
      721 tcagtaatgt gccagtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
      781 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
      841 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgcgtcc
      901 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
      961 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctgccaa
     1021 atgataatcc tgcaccggag caatatcaga agcaatttaa taccggtagt caaacagcag
     1081 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     1141 tgaagaaaaa cggacagtat gacaatacta ttattctctt tacctccgat aatggtgcgg
     1201 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacttatc
     1261 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa ccaggtaatt
     1321 atgacaagct gatttccgca atggatttct acccgacagc tcttgatgca gccgatatca
     1381 gcattccaaa agaccttaag ctggatggcg tttccttgct gccgtggttg caagataaga
     1441 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgacg
     1501 aggaaaatat tccattctgg gataattacc acaaatttgt ccgccatcag tcagacgatt
     1561 acccgcataa ccccaatacc gaggacttaa gccaattctc ttatacggtg agaaataacg
     1621 attattcgct tgtctataca gtagaaaaca atcagttagg tctatacaaa ctgacggatc
     1681 tacagcaaaa agataacctt gccgccgcca atccacaggt cgttaaagag atgcaaggcg
     1741 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     1801 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     1861 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     1921 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     1981 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gcagcgtctg
     2041 gcaatcaggt ctattttacg tggcaaggcg gtgaacccac tctggctggt ctggattttt
     2101 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     2161 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgagt
     2221 ttctggttgg tatctcgatc gatggacccc aggagctaca tgaccgttac agacgcagta
     2281 attctggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     2341 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     2401 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctcgaaa
     2461 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     2521 tttctgtgcc tcctacggct tatggcaagt ttatgtcgac catttttatg caatgggtta
     2581 aaaacgatgt gggtgaaatt ttcatccgtc agtttgaaag ttttgtcagc cgttttttgg
     2641 ggaatgggca taccagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     2701 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     2761 ttaataaatc tgaacttaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     2821 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgccctaagc
     2881 atcgtattac taaagtaaac aatgagactg tttcctattt ttgcgaaggt tataaaatcc
     2941 ttttttcaac catggtacct tatatgaacg ccatggtgga gttagccaag aacagagtac
     3001 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctccttct
     3061 ttgctccagt cttagcctcc gtcttatgac cttaacaaag cacccgacat aaattcctgt
     3121 tgggtgtttc gtcatcatca tcttcccttg cgaaaattct ggcttacgtg gcatgcgtca
     3181 gggcattagc aataccct
//
LOCUS       DAYPHN010000263         3186 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000263 DAYPHN010000000
VERSION     DAYPHN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.265"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..511)
                     /gene="artQ"
                     /locus_tag="ACG0BW_004663"
     CDS             complement(<1..511)
                     /gene="artQ"
                     /locus_tag="ACG0BW_004663"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFK7261514.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWL"
     gene            complement(518..1249)
                     /gene="artI"
                     /locus_tag="ACG0BW_004664"
     CDS             complement(518..1249)
                     /gene="artI"
                     /locus_tag="ACG0BW_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFK7261515.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(1267..1995)
                     /gene="artP"
                     /locus_tag="ACG0BW_004665"
     CDS             complement(1267..1995)
                     /gene="artP"
                     /locus_tag="ACG0BW_004665"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFK7261516.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(2213..2728)
                     /locus_tag="ACG0BW_004666"
     CDS             complement(2213..2728)
                     /locus_tag="ACG0BW_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFK7261517.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          808 a          783 c          798 g          797 t
ORIGIN      
        1 ccagccactg gttaccgagg ccaggcagcg catgacgcca catctgcggc atcaccagac
       61 ggaaaaagat agccgatttc gacagcccca gcgcctggcc ggattcccac tgacccatcg
      121 gcaccgcttt caacgcgccg cgcagcgttt gcgaggcata ggcggcatac agcagcgaca
      181 gagcgatgac accacaaagg aacgggctaa cgtcgaagtt ctcaatgtcc atctgcaccg
      241 ggatctgcac gaacccaaga ttgatagtga agccatccga aagcgtcagc agcagctgcg
      301 aggagccaaa atagataaac agcaccacca gaatttctgg caggccacgc agaatggtta
      361 ccagcgctga acctgcccac gcgacaggac gccattttgc cgactcccat accgcaaaga
      421 acatcgccag cgccagcccg acaatcaatg cacaaacggc aaggccgacg gtcatcccgg
      481 cggcgcttgc taaaggaaaa aattcattca tcaggaatta cttctggaac catttgttgt
      541 agatggtttc gtaagtgcca tctttcttca ctttttccag cgcagtgttg agtttctgct
      601 gcagttcagt gttgccctga cgtaccgcga tgccgaggcc agtaccgaaa taatctttat
      661 cggtcacttt atcgcccact gccgccagtt tcgggttgtc tttcagccac tcggtgacca
      721 ctgcagtgtc accgaatacg ctgtcgatac gaccgttttg cagatccagt tttgcgttct
      781 ggtagctgtc atacggaacg gtggtgattt ccgggtgctt atccataatg aacttctggt
      841 gtgtcgtccc gttctgaacg ccgacttttt tgcctttcag ctgatcaaca ctggtgtatt
      901 tgccttgctg acccacaaac agggcagagt tgtcatagta cggcgtggta aacagcacct
      961 gcttttcacg ctccggagtg atatccatgc ccgccatcac tgcttctacg cgacggaatt
     1021 tcaggcttgg gatcaggctg tcaaacgcct ggttagagaa agtacaggtt gcatcaatct
     1081 ctttacacag cgcctgtgcc aggtcgacgt caaaaccaac gatctggttg tttgcatcaa
     1141 tcgattcaaa cggaggatag gaggcttcgg tagcaaaacg aatggtttcg gcagctgtgg
     1201 cggaaagact aaaacctgca attaacgcgg caatcagaac ttttttcatt gttgttatcc
     1261 cgaatattag tgagagagat agtttttaaa tgcttcggtt tgcggctcgg taaagcagct
     1321 cgcgtcgcct tgttctacga tatgaccgtt ttccatatac accactcggc tggcggtttt
     1381 acgcgccact tcaacttcgt gggtgacgat cacctgggta atattcgttt ctgccaactc
     1441 acgaatgatg ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg tcggttcatc
     1501 gaacagcagt acctgcggtt ccatcatcaa cgcacgggca atagcaacac gctgctgctg
     1561 accaccagaa agatgcagcg ggtaacgatc gctataaggt ttgagacgca gacgttccag
     1621 cagtttttct gcacgggcca gcgcctgatc tttgctcaac cccagtacac ggcagggcgc
     1681 ttcaatcagg ttttgctgca cggtcagatg cggccacagg ttgtattgct gaaacaccat
     1741 gccaacgtta cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg tgaaatcgaa
     1801 atggttgcct gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat tgagtacacg
     1861 cagcagcgag cttttacccg cgccgctggg gccaagtaac accagcgttt cgccctgtgg
     1921 gcaatccagc gtgatatcga acagcgcctg atgcgcgccg tagaagcaat taatgccgtt
     1981 taattgaata ctcattgaca ctcgtatact ggcagtctga tagctattga ggtcgaagat
     2041 agtacctttg acagaataat tatgcaatat ttctgcttta aaagttaaaa gcaaagcgca
     2101 ttattcaata aacatagcac aaaataacgg gggcggtggt cggcgagcat aaatgtcggc
     2161 attcctcacg aaatgccgga caatttacgg ggtttattgg ttgatcaagg ctttagcgat
     2221 tctcgatgga ctgacggagc gtacccgctg tggcgtgaac gctaccgcct aagtagcgca
     2281 catcgtcgat gacccaacac tggccttcct gaatcattaa cacttcatcc tgccatcctt
     2341 ggtcaccctg tttgagatcc acgcgcaatg gaatgttacg ggcatcacga ttagggatag
     2401 tcgatgcact ggcaacgtgg gcgctgtctg gcaaggtggt tcgactggag aacggatcgc
     2461 tgcttaacaa ttcacgatga ctattatcgc ggctggcatc gctaagtagg gttgccagtt
     2521 tgtcactcaa ataggggcgc agggcggtaa tgtcattgct gcgatgttga atgcggtagt
     2581 catagaattg ttgagccacg ctgtcagggc caccctggac acaggaaccg gtgcgggggc
     2641 cgttatcttt ataagctggc gtgacggtgg tacaggcact cagcagcagt gcacatggaa
     2701 taagtagaga tagtgaacgg tagcgcataa tcatttcctt attagcttgc tgtctgcgtc
     2761 acactacgac caaacgttat cagattattt aatacattgt ttttaataca attctgaaac
     2821 aatctgaaca cctgattggg tttgagtcca ttgctggcta cccactcaag gttgctcgta
     2881 gctgacttac ttaaattacc caaacaatag acagtgtaaa ccatttgtga ggttgagcgt
     2941 gttatatggc tgaaatgatc atttaaggta aatatgatag ttctgaaatg gcgggataaa
     3001 tcctcctgta gaggttaaga gcatctgcaa gaagccagtt tcacatttat aacgcgtgat
     3061 aaatgaacgc ggttattgtc taatcattac agaatttttc acatgcaata agagatcctg
     3121 cgattaagat gattgctaaa attacattgt atttttgaaa atgagcgaaa tttgatagtc
     3181 ccaact
//
LOCUS       DAYPHN010000264         3166 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000264 DAYPHN010000000
VERSION     DAYPHN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.266"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(4..630)
                     /gene="dsbA"
                     /locus_tag="ACG0BW_004667"
     CDS             complement(4..630)
                     /gene="dsbA"
                     /locus_tag="ACG0BW_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFK7261518.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(647..1633)
                     /gene="srkA"
                     /locus_tag="ACG0BW_004668"
     CDS             complement(647..1633)
                     /gene="srkA"
                     /locus_tag="ACG0BW_004668"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFK7261519.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1710..1979)
                     /locus_tag="ACG0BW_004669"
     CDS             complement(1710..1979)
                     /locus_tag="ACG0BW_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFK7261520.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2049..2633
                     /gene="mobA"
                     /locus_tag="ACG0BW_004670"
     CDS             2049..2633
                     /gene="mobA"
                     /locus_tag="ACG0BW_004670"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFK7261521.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQETGEWFLFCPCDT
                     PYIPPDLAARLNHQRKEAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            2615..3142
                     /gene="mobB"
                     /locus_tag="ACG0BW_004671"
     CDS             2615..3142
                     /gene="mobB"
                     /locus_tag="ACG0BW_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFK7261522.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVSLNLDVALLDINDVG
                     GLADFVVEWMQNQDG"
BASE COUNT          829 a          773 c          771 g          793 t
ORIGIN      
        1 gaattatttt ttctcggaca gatatttcac cgtatcagca tactgctgaa caaaaacatc
       61 catattgctg gtatccatac cctgcggatt cagctgatat ttaccgttaa caaacatcgc
      121 cggaacgcca cgcaattgca cgtcagctgc agctttttcc tgctgagcga ccagggattt
      181 caccacgaag ctgttccacg ccgcgtcgta ctcttcacct ttaatacctg cgttgataaa
      241 tacatcgcgg atatcagatg ctgaacgaat ggtctgggtt ttctgtacgc cttcaaacag
      301 cggaacagtg actttgtctt ccacgcccag tgccatcgcc actgcccatg cctgagtcag
      361 atctttgccc aggtcaccac ccatgaagtt gacgtggtat ttagtcatct tcacgccttc
      421 cggcagtttt ttcttcacgt tatcagaaat atgcagaact tcttcaaact gatagcagtg
      481 cgggcagaag aaagagaaaa actccagcac ttgcggcgcg ccagcaactg gtttttccag
      541 ggtagtgtac tgtttaccat cttcatactg cgccgccgat gcgctaaacg ctaaaactaa
      601 accagccagc gccagccaaa tctttttcat gatctactct ctccgattaa tacataggtg
      661 ttaattgcaa agggggttct tgtagaactt ttgcctgttc tataaaagtc gccgtctgtc
      721 gcagccagta atcttccccg gttaaccacg ggaaattttt cgggaacgcg ggatcagccc
      781 aacgccgcat cagccaggca agataataaa ccaaacgcat ggcgcgtaaa ggttcaatca
      841 gtccgatttc agcggtgtcg aactcgctaa attcttcata agcttcaata atagtttcca
      901 gttgcatccg ctgctcggct ttatcgccat tgagcaacat ccacaaatcc tgaacggctg
      961 gaccattacg tgcatcatcc agatcaacaa acattggacc atcgcgccag aggatattac
     1021 cggcgtggca gtctccatgt agccgcagaa cggtgaaatc ttcccgccag tgtgctgtaa
     1081 cggcggcgat cagctcatct gtcgctttca ggaatgccgc tttcaacccg gaaggtatca
     1141 gtgtagcgtc ctcaaacagc ttgcgaggct caatgagata ttcattcaga ccgatggtcg
     1201 ggcgatggat aaaaagctgt ttgcgccccg tctgatgcat acgccctaaa taacgcccaa
     1261 ccgcttccat ctgatcgata ttatcagctt cgaactggcg accaccgacg cttggaaaaa
     1321 cagcgaaata aaatccctga tgattcaata aagtctgacc gttaaaggcc acaggcgctg
     1381 cgaccggaac ttcatcgtta accagctgca acgcaaattg atgttcttcg aggatttgat
     1441 cggctgtcca acgttcaggg cgataaaatt tgacgacaaa acgtcgacga tcttcgtcct
     1501 gaaattgata gacacggttt tcatagctgt taagcggggt aagaccggaa tccacccgga
     1561 tcccttgctc aaatagagca tccatgatgg tatccgggtg tagtgtctgg aaagtaaaag
     1621 cgctgttgtt catcccatca tccggaaaac attacgaatg atgaaggata tcatttcacg
     1681 atgattttgg tggcgccgct tacaagcttt tactctttaa ttacgccgcg agcgcgtaac
     1741 agcgccgttt tgaaatcttc ttcataatct ttctgcaaac ccgggatcac cgcatctttc
     1801 gcggaatcac gcattttcag atgatagatc agaatgtcat ccgtcaaatc cgccagttcg
     1861 ccgtcaaaac ctgactcttt cgccagtttc tgcaaaaatt gcagcaggtt aaggtctggc
     1921 tctttttgcc aggctggctg gaggagttca ataacttcat tcagacgttt acatttcata
     1981 gtaatgctcc ttactcttga gacagacacg ttagcagggt caatcccaca ataaaagagg
     2041 cgatatcggt gaatctgatg acgacgataa caggcgttgt gctggcgggc ggtaaagcca
     2101 gacgaatggg cggcgtagat aaaggattgc ttgaattaaa cggcaaaccg ctgtggcaac
     2161 atgtcgctga cgcgcttatg acgcagctct cgcacgtcgt ggttaatgcc aatcgtcatc
     2221 aggaaatcta tcaggcaagc ggtctgaaag tgattgaaga ttcacttgcg gattacccag
     2281 gccctctggc aggaatgctt tcagtaatgc agcaggaaac gggggagtgg tttttgtttt
     2341 gtccgtgcga tacgccttac attccccctg atttagcagc ccggcttaat catcagcgca
     2401 aagaggcacc tgtcgtgtgg gtccacgacg gtgaacgcga tcacccgact attgctctgg
     2461 taaaccgcgc aattgagcct ttattactgg aatatctgca agcaggagaa cgccgggtaa
     2521 tggcatttat gcgtctggct ggcggtcatg cggttgattt cagcgataat aaagatgcat
     2581 ttattaacgt gaatacgcca gaggagcttg cccgatggca ggaaaaacga tgataccgtt
     2641 actcgccttt gccgcgtgga gcggcactgg aaaaactacg ctgctgaaaa aactgatccc
     2701 ggcattatgc gccagaggga tccgtccagg gctgattaag catacgcacc atgatatgga
     2761 tgttgataaa ccgggcaaag atagctatga gctgcgcaag gctggcgcgg cacaaaccat
     2821 cgttgccagc cagcagcgat gggccttgat gacggaaaca ccagacgaag aagagctgga
     2881 tctacatttt ctcgcaagtc gaatggatac ctcaaagctg gatttgattc tggtcgaagg
     2941 gtttaagcat gaagagatcg caaagattgt gctttttcgc gatggagccg gacatcgacc
     3001 ggaagaatta gtgatagaca gacatgttat tgctgtagcc agtgatgtgt cacttaatct
     3061 tgatgtcgcg ttactggata ttaatgatgt tggggggctg gctgattttg tggtggagtg
     3121 gatgcaaaat caggacggat aagcaagagt aggctggatc agacgc
//
LOCUS       DAYPHN010000265         3134 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000265 DAYPHN010000000
VERSION     DAYPHN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.267"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..827
                     /locus_tag="ACG0BW_004672"
     CDS             <1..827
                     /locus_tag="ACG0BW_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFK7261523.1"
                     /translation="QMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKP
                     ATTQGKRFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEH
                     YNVLEQDLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISS
                     WYTDEYKKLFALPDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNR
                     KKLNALLNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(1149..1751)
                     /locus_tag="ACG0BW_004673"
     CDS             complement(1149..1751)
                     /locus_tag="ACG0BW_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261524.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
     gene            complement(1846..2052)
                     /locus_tag="ACG0BW_004674"
     CDS             complement(1846..2052)
                     /locus_tag="ACG0BW_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7261525.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2795..2962
                     /locus_tag="ACG0BW_004675"
     CDS             2795..2962
                     /locus_tag="ACG0BW_004675"
                     /inference="COORDINATES: protein motif:HMM:NF017162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261526.1"
                     /translation="MIFHTRYRLTPVDLEARNSAVAHRLTELTANFTSACRASVWSPE
                     RSVTEARAVIG"
BASE COUNT          809 a          712 c          842 g          771 t
ORIGIN      
        1 aacagatgat tgaaaatgcc agaatgacgt tatccctcat ggtgcaaccc ggtctttttg
       61 accgatatat ggagcgaaaa ggttctgttg ccagggacag tggttttctt gcccgttgtc
      121 ttatcagtaa acctgcaacc acgcagggga aacgctttat taacggagct gtgattccgg
      181 gaggatcatt aacggcattt catgaacgat taatggaact ggccagaggc agtattgaaa
      241 aatccagtga ggatgaacgg tattgtctgc atttttcgcc tgaagcgcaa aaaatattta
      301 ttgagcacta taatgttctg gagcaggatt tatccccttc cggaccctta agcccgtttc
      361 ggggacatgt ttcaaaaaaa acagaaaaca ttgcaagaat tgcagcgtta tttcagtatt
      421 tcagctatgg tgaagggaaa atctctgccg atatcatgac gtctgctgtc gtaataagtt
      481 cgtggtacac agatgaatat aaaaaattat ttgcgttacc tgatgaatct gaactgcagc
      541 agaaggacgc cgaggagctg ttcgactggc tcattgagga gtgccgcgga gaatgcccgc
      601 cccgtgtcag gaaaaattat attctccagt gtggcccggg ccggttcaga aacaggaaaa
      661 aactgaacgc attgctgaac attcttgaat ctcagttcag gctttctgtc gtgccggaag
      721 gaaaaaccat gtatgttctg cttccccaga tagccagttt aaagttgtct gatgtctctg
      781 gtatttttac ctcgggttat cactacaata agctgagagc gaagtaaccg gtgagcagtt
      841 tttctccatt atgcttcaga cttagatgat tgattattat ggggatttca tcaccgatat
      901 tcagatgtat gaatatcggt gatgaactaa agctcaggag ctgcgtggtg gtgatgaatc
      961 aacaggcata taactgatac ccgccggtca gaagttccag cctgcgactt tcctgtctgc
     1021 gtgcccgcag tccggcgttc aggtgcagcg tgtgacagaa cgcacggaga gcgaaacggc
     1081 aggcagacag gccgttcagt cagccctggc accctgcccg gataccagct tccctgaaac
     1141 aactggcatc aggtgcgctt atcgtcaacc gttgtgcggt atgacaggag cacgtactga
     1201 tccatcagat ccgtcagtgc tgcgctctgc ttctgacggt tgatgatcca ttcctccagc
     1261 tctttttctg atttccgttt cagattctcc gtccagtcgt caaggcagtt tccggtcatt
     1321 aaatcaagcc agcaaagcca gaccagtttg aggcgcaact ccgtgccacc aaactgacgg
     1381 aagaggctga tcagtttatt tgccagagaa cgctgcctca gacgttcaca ttcagacagg
     1441 agaatgcctt ccagcatcgg atgatgaacc agcagatgtt cactggtgca taaattcact
     1501 atcagatcat catccagggg cggtgtggcc cgtttgtcag ggccataaat cagccggtgc
     1561 aggctctggg gtacgatggc aatctccggt ttttcaccat tgaatgacac ccaaccgggc
     1621 gtcagattat ggaagtagta gatattcgga tgacacacgc cccaggatac ggcgtgccgg
     1681 gtgaagccgg tcagccattt ctggtagctt tcgtggagcc attcgtagcg ggtctgatac
     1741 tgttgtttca ttttcatatt cctcacgggg atataacagg cagcagaatg ccgccggcag
     1801 gccggagatt acgtctgctg cgggtgaacg gataacggtc agaaattacg gacgggactg
     1861 tgcaatacgc gcctgcagcc aggcttccac ttcacttttc agccagcggg agctgcgccc
     1921 cattttgatg ggggcaggaa agcccccatc tttgatgagt ttgtaaaacc acttatcggt
     1981 taagccggta agttgagtga taaacgccat gtcgaccatc tggtcatcca tcagcgaaac
     2041 aggggtagcc atgagaatgt tcctccggat gttgttaaac cagccggtgt ggtcatgaaa
     2101 acagcgcgcc accttcactg gcataacaac cggggagaaa cgtaaaaaat aatcgggaat
     2161 gtggctgtcc ggagataaac ggggggattg cgatggcaga gccgtagcgg aatcccgggg
     2221 ggttgttgcg aaaagatgct tccgcacgca cgcgcgataa aggagggagg actttcgtga
     2281 gacaagggga aaactagcgt tctgataatt gcagagttgt tttcggaggt ggttacagtg
     2341 agtcagcccg ttcctggcga ggaggagact gatatcaccg atgggataag gctgtctgat
     2401 tggtattata aatataagaa gtcatcagag agtaacgtaa aggatttgcc agagtaagct
     2461 aagagagtaa atctctccgg taacagagcg ctgtgaagca ttctgtatca gagtgcaaat
     2521 acatgaacgc tgatgcgtaa aatcagcagt gacacgccag ctccccctgt gcccattcct
     2581 gtataagaca tgaactgagc gtattaatgc tcctgctctg agactgagcc ggtgagtgag
     2641 gggctgagaa atccctcttg acacagctct aaaccagaaa tacttccccg gttgttgcgg
     2701 tgcgtccgga tccccgataa cggaacacta aggctgtatt gcgggacacc gggtaagtgg
     2761 cagcctgtaa tgtgttctgt caacgccaga aaaaatgatt tttcacacgc gttatagact
     2821 cactcctgtg gacctggaag cccgtaactc tgcggttgcc caccgactga cggaactgac
     2881 cgcaaacttt acgagtgcgt gcagggcttc agtctggtca cctgagcgtt cggtaacgga
     2941 ggcgcgggcg gtaataggct gattttcagg tattaccggc ttacagcgtg tggttgatat
     3001 tgaggggctg tgagtttcgc gtatttgtca gaaacccaga cgagttttcc tgtatgggac
     3061 gaaataaggc agcggcaata cctggaaaaa tttaatatgg tatgaaggag aacctgtcgg
     3121 ttacgcttaa tata
//
LOCUS       DAYPHN010000266         3065 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000266 DAYPHN010000000
VERSION     DAYPHN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.268"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1275
                     /gene="sctN"
                     /locus_tag="ACG0BW_004676"
     CDS             <1..1275
                     /gene="sctN"
                     /locus_tag="ACG0BW_004676"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFK7261527.1"
                     /translation="LNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANERIARAQ
                     VVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFVP
                     DTPGERGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGK
                     TVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDR
                     CNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDS
                     LPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYP
                     AIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNAENDFAM
                     NARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1265..1723
                     /locus_tag="ACG0BW_004677"
     CDS             1265..1723
                     /locus_tag="ACG0BW_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFK7261528.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLVEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            1701..1931
                     /locus_tag="ACG0BW_004678"
                     /pseudo
     CDS             1701..1931
                     /locus_tag="ACG0BW_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            2229..2576
                     /locus_tag="ACG0BW_004679"
     CDS             2229..2576
                     /locus_tag="ACG0BW_004679"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFK7261529.1"
                     /translation="MFTQHIEKQRNIHTLNQNDINNSVNSANVPENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDTIVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHDEQE
                     NEGKFENDQKDES"
     gene            2536..3065
                     /locus_tag="ACG0BW_004680"
                     /pseudo
     CDS             2536..3065
                     /locus_tag="ACG0BW_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         1043 a          537 c          706 g          779 t
ORIGIN      
        1 ttaaataaat acagctactt acatagcata aacggctcgc tgattgaagc agagcttgac
       61 gacgtttcag tgggcgaagt atgcgaaatt tatgcgagct ggcaagcaaa tgaacgtata
      121 gccagggctc aggtcgttgg ctttcgcaat ggaaagacat tattaaacct tataggcagc
      181 tctgtggggc ttacacgtac tgcagttttg aaaccaacgg gagagcagtt aaccatacag
      241 atcagtgatg cctttcttgg ttcagttttg aatgcttctg gtcaaattat ggaaaggttt
      301 gtcccggaca ctccaggcga aaggggaaat ttacgcctga ttgatgaact gccaccgtcg
      361 tatcaggaac gtcgagttat taatacacca ctagagacac ggataagagt cattgatggc
      421 gtactgacat gtggcattgg tcaacgtgtt gggattttcg catcggctgg ctgtggcaaa
      481 acggttttaa tgcatatgct tgtaaacaac actgaagctg atgtgtttgt tattggcttg
      541 attggcgaac gtggaaggga agttacggaa tgcgcggaat cgctgaaaaa atcagtaaat
      601 gcagcaaaat gtgttttggt ttatgccacc tctgattttt cgtcagtaga tcggtgtaat
      661 gccgccctga tggcgacaac cgtggcagag tattttagag atcgtggaaa gagagttgta
      721 ctttttatcg actcaatgac gcggtatgca cgagcactac gcgatatgaa gctggcagcg
      781 ggtgaaccac ctgccaggcg tggttatcct gcttctgttt ttgacagttt acctcgctta
      841 ctggagaggc cgggaccaac attaaaagga agtattactg cgttttatac cgtattactg
      901 gagggggagg atgaatcaga cccactgggt gatgaaattc gctcaatcct tgatggccat
      961 atctatctaa gtcgcaaact cgctgggcaa ggtcattatc cggcaataga tgtactaaaa
     1021 agtgtgagtc gagtatttgg tcaggtcact gatgaaaaac acagagataa tgcagcacgg
     1081 gtaaggaaga tcctcacaac tttggaagat ctacaggtgt ttattgattt aggggaatat
     1141 cgcgctgggc aaaatgctga aaatgatttt gctatgaatg cccggcctaa actcacaaat
     1201 tggttaaaac agtcagttaa tgaaaaaatg ccaatgagtg aaacgctaaa agagcttgaa
     1261 cgaattgtta agtaaagtta atcgacttat cagaagaaca gcgcagagcc tggctgcatg
     1321 tgaagcatca ttacaaaagt taaatgcaga aaaagaaaag ttagtcgaaa aggaacgcct
     1381 gtatgatatg caacttaaaa acctacagtc actgttagat atgaaggaat tactcggcga
     1441 agttgtattt cgccaggata tattttactc gttacgtaaa gttgcagtca ttcagcagca
     1501 gatcgctgag ataaatcttg aaaagcaaaa aatagcagag cggcgaaaaa tacttaacaa
     1561 ggaaatagtt caacagcaag cacaaagaaa acactggtgg ctaaaaggag aaaagtacga
     1621 tcgactaaag aagcgcatca aaaaacaact tttaaatcag atgctttatc aagacgagct
     1681 tgaacaggag gagaaatata atggacgaag tcaagaaaat tgaacattat aaaaatttaa
     1741 acacagacac atccgagaat tctgctgcgg atttagacag tttcttaaag aaaaacaaaa
     1801 aaaataaaga aatgagtgag attgttgctg gatccattct ccagttatta cgtcaggctc
     1861 aggatagcca attccttacg tttgggaaaa agaatgggca aatgaagtct attgataaca
     1921 aatcgtctct ccaggttgac aggaattctt ctttttcttt gcaaacaaat ggaatagagc
     1981 aactttctac aaagaaagtc ctcaatatga cttcacgaga tcagggtgtt aattctgaac
     2041 tatctgataa agcgatacaa tttaaggggg aaatccataa tggtatccat acagaaaaca
     2101 tcacggttca aaaccacagt aataataaag agcgtgaaaa aaaatatcgc gatggagaca
     2161 aaaaaaatgg agagcaggcc catttattag atattactaa cgaaagaagg tttgctgata
     2221 acaaaaccat gtttactcaa catatcgaaa agcaaaggaa tattcatacg ctcaatcaga
     2281 atgatataaa taattctgtt aatagtgcca atgtacccga aaatgaattg acatatcagt
     2341 ttcagcgttg gggacagaat catacagttc ggattctgga aagtagtgaa gggatacgac
     2401 taaaaccatc agacacgatt gtctccgaca gactccatga agcccaacac aatgatgtta
     2461 cagcacaacg ttgggtattg actgagcaag atgaacgaca agggcaacga catcagcctc
     2521 atgatgagca agaaaatgaa ggtaaattcg aaaacgatca gaaggacgaa agttaatgtt
     2581 cggtctgaga aaagtaaatg gaaacactca ttcctttgaa accacttttc aaaattggaa
     2641 agaaaagggt gaagatgttg cgttattaat gcccgaattt agcgccaaat ggctaccgat
     2701 agcagaagag agcggcagct ggtctggatg ggtgttactt cgagagttat ttcccttaat
     2761 ttctgccgag ctcgctggga tggctttaat gccagaaacg gaaaggttaa taggggaatg
     2821 gctcagttta tctagtaccc cgttaaatct aaagtatcct gaactaaaat ataatcgttt
     2881 gtgtgtcggt aaagtatttg atggagtatt gagtccagct caaccactga taagaatatg
     2941 gacaggggaa ttaaatattt ggttagataa agtcacagtc tgccaatacg aaaacgctcc
     3001 aacgctagac aaaaaatcat tatattggcc cattcatttt gtaattggat ttagtaaaac
     3061 atgtt
//
LOCUS       DAYPHN010000267         3061 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000267 DAYPHN010000000
VERSION     DAYPHN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.269"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..174)
                     /locus_tag="ACG0BW_004681"
     CDS             complement(<1..174)
                     /locus_tag="ACG0BW_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFK7261530.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTF"
     gene            complement(174..422)
                     /locus_tag="ACG0BW_004682"
     CDS             complement(174..422)
                     /locus_tag="ACG0BW_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFK7261531.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(454..1368)
                     /locus_tag="ACG0BW_004683"
     CDS             complement(454..1368)
                     /locus_tag="ACG0BW_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFK7261532.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(1365..3053)
                     /locus_tag="ACG0BW_004684"
                     /pseudo
     CDS             complement(1365..3053)
                     /locus_tag="ACG0BW_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
BASE COUNT          769 a          804 c          789 g          699 t
ORIGIN      
        1 aaaggttaaa ccgagataat tattggtccc ggccagaatc acttttttat tgccgatccg
       61 gccttcggtg gcggaataca cttcatcaat acaggtgcca aacggtgtta acccgttgtc
      121 actaaattgc agtcgctcgc cagcaaggcg cgcatattta tcgtatagcc ccattattta
      181 ttctccagcc atggaagtag agtttccatt aattgcgcag gggttttgac atccagcaga
      241 atattaatag gaatggaaat atcgaatctg tcttccagca tcattaagag atccatcact
      301 ttgatcgact ccaggccaag atcgttgacg agatcgctgt cgggcttaat ttccacaccg
      361 ttttcgacca aatcctgtaa acaggaaagg atgtaatcca ttactatttc acgatttacc
      421 atagaaacgt taacacacct ttaaacaaga caatcaaaat agcccgttgc gtaatgcgtg
      481 ttccaggcta atcccgggaa accaattaat atcttcagaa atacggttat tacttgccga
      541 ccagtcggcg tgggttaatt cacgaatttt cgagcgggtc agcataggtt ctttacctgc
      601 caggcgactc aacgcggtac tgatatccgc aaggcaggtg agtaccggca gaggaatacc
      661 aaccattcgc acggaaccgc aacggacgtt ggcggcaagt tgctgtatgc gttgccagtc
      721 atagccgcca gcgacgccat cgcataattc ataggtttgc gtctgtatag tttcggcgct
      781 taaccactga cccactgctt gcgcgaaatc ggtgacgtgc agaaaagaga gctgtgtatc
      841 tggcgcacca agtcgtggca gcaggccgcg cagcatccag tcaaacagcg gttttaactc
      901 tttatcgccg ggaccataca cggctgtcgg gcgaaaaacg cccagcgtaa tttcgtcagc
      961 cattgcagtc agccgttgtt cggcgatgtg tttggaattt gcgtaccagg agagctcggg
     1021 atggcgcgcc gccagcgaag agataaacag aaaacgttgg caaaagcagc tctcttttgc
     1081 tgcttgcatc aggcgcaggc tgccgtcaac gttacagcgg gtgaaaatct cttctttgtg
     1141 cccgcgcact tgcccggcgc aatggaccac tgcgctggct ccggcaacca gcttgctaag
     1201 tgaatgagta tcttccagcg aaccgcgcac ccaggtgaga ttatcattga cgtgagcgcg
     1261 ggcagtacgc gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat cgataatata
     1321 tttaccgata aacccggtag cgcccgtcac cgcgacagtt tgattcatgc cagggattcc
     1381 tgcacattaa gactggcagc ataagccttc tgataacgtt ttttcgctta cgcacgggca
     1441 ggcttgccgg aggacgttcg gggaatactg tggggcggca acagatcgat agccgcggtt
     1501 acgccaaatt cgctttggat ccgtgccgcc agcgcgtgga taagctgccc gcgacgttct
     1561 tcgtcgctga tccgacactg gatctgcaaa atgatttttt cctggacggt aacaaaagca
     1621 atcgcatcgc cagaatgaat ttccggttct tgttccgcaa tatattcaat atcctgcggc
     1681 cagatattac ggccacgaat aataatcaga tctttaatac gtccggtgac atacagataa
     1741 ccgtccagca gataaccgag gtcgccggtg tctaaccagc ccgtcgcggc aatctcgtct
     1801 tgcgaaacct ggtcgccaaa gtaaccgctc atcaggctgg ggccggagat gcaaatatgg
     1861 cctaccacac gttccgcgac cggcatacct gcttcattgc ggatttcaat gccatgttcc
     1921 ggcaacgctt tgccgcagtt gacgaaagtc gatacggcgc gtgtttcagc acccggcgcg
     1981 acggctttgc cctgatattc taggatgtcg cgatccactt cgttaaccac aaccccagag
     2041 ccttcatcag agaagctgac agccagcgca ttttctgcca gtccgtagca cggcatgaac
     2101 gttttatcgt caaagttaac ctgacggaaa cattcagcga attgatggag ttgttctgcg
     2161 gagatcggct cagcaccaat accagcgacg cgccagcagg aaagatccag ttcagcgaga
     2221 tctttttcat tcacgcggcg ctggcacaat tcatagccaa acggcggcgc aacggaaacg
     2281 gtgccgcgat ttttactgat caatttaagc cattgcagag gacgcatggc aaaatcctga
     2341 gtgcgcaaat aatctactga aagttgcgtg gcgacggggg tcaggagaaa gccgaccagt
     2401 cccatatcat ggtagaaagg cagccaggag acgcagcggt cgccagggcg taatttaatg
     2461 ccatcgtggc ttatagcacg tagattagcc atcacttcgc gatgggtgat aatgacgcca
     2521 cggggaaaac gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt cggaactgga
     2581 cgctggagcg caacatcggc ttccggtaat gccttaaacc aggcgtggct taaaacatgt
     2641 aattcggggt tgttatgcgt cgcggcattg accagtggca accactcatc accagtgata
     2701 atggctgcgg gctggcaact tgccagtaaa ccctggagtt tggcgctcca ggaatcccgc
     2761 tgaccgacgc ccattggaat cgccaacggg acggcgacta agccagcata ctgacaggca
     2821 aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg ttcgcctttt
     2881 ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgtttt taacgtctga
     2941 tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc ggcgctactc
     3001 agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg cataggtagg
     3061 g
//
LOCUS       DAYPHN010000268         3021 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000268 DAYPHN010000000
VERSION     DAYPHN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.270"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            6..1226
                     /gene="astC"
                     /locus_tag="ACG0BW_004685"
     CDS             6..1226
                     /gene="astC"
                     /locus_tag="ACG0BW_004685"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFK7261533.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLHGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTVNHRCGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACDHFVSRGSS"
     gene            1223..2257
                     /gene="astA"
                     /locus_tag="ACG0BW_004686"
     CDS             1223..2257
                     /gene="astA"
                     /locus_tag="ACG0BW_004686"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFK7261534.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2254..>3021
                     /locus_tag="ACG0BW_004687"
     CDS             2254..>3021
                     /locus_tag="ACG0BW_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFK7261535.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVA"
BASE COUNT          639 a          770 c          934 g          678 t
ORIGIN      
        1 tcgctatgtc tcagccaatt acgcgtgaaa actttgatga atggatgata cctgtttacg
       61 ctccggcacc gtttataccg gtacgtggcg aaggttcgcg cttgtgggat cagcagggga
      121 aagagtatat cgacttcgcg ggtggcattg cggtgaacgc gctgggccat gcgcacccgg
      181 aactgcgtga ggcgctgaac gaacaggcga gtaagttctg gcataccggc aacggttaca
      241 ccaacgagcc ggtactgcga ctggcgaaaa aattgatcga cgccacgttt gccgatcgcg
      301 tcttcttttg taactccggt gcggaagcca acgaagcggc gctaaaactg gcgcgtaaat
      361 tcgctcacga ccgctacggc agccataaga gcggtatcgt ggcgttcaaa aatgcgtttc
      421 atggtcgcac gctgtttact gtcagtgcgg gtgggcagcc cgcctattca caggattttg
      481 cgccactgcc gccggatatt cgtcatgctg catataacga tattaactct gccagcgcgc
      541 tgattgacga ctctacctgt gcggtgattg tcgaacccat ccagggggaa ggcggtgtgg
      601 tgccagccag caacgcattt ttacatggtc tgcgtgaatt gtgtgaccgc cacaatgcat
      661 tgctgatttt tgatgaagta caaactggcg tcgggcgcac cggggaactg tatgcctata
      721 tgcactacgg tgtgacgcct gatctgttaa ctaccgcgaa agcgctgggc ggcggtttcc
      781 cggtcggtgc gttgttggca accgaagagt gcgccagcgt gatgaccgtt ggcactcatg
      841 gcaccaccta tggtggtaat ccgctggcct cggcggtagc aggcaaagtg ctggatctca
      901 ttaacacgcc agagatgctt aacggggtta agcagcgtca cgactggttt gttgagcgtc
      961 ttaataccgt taatcaccgc tgcggtttgt tcagtgaagt tcgcggctta ggtttgttga
     1021 ttggctgtgt actgaatgcc gattacgccg ggcaagcgaa acagatctct caggaagcgg
     1081 cgaaagcagg cgtgatggta ctgattgcgg gtggcaacgt ggtgcgtttt gcgcctgcgc
     1141 tcaatgtcag cgaagaagag gtgacgaccg gactggatcg ctttgcagct gcttgcgacc
     1201 actttgttag ccgaggttca tcatgatggt catccgtccc gttgagcgct cagatgtctc
     1261 ggcgctgatg cagcttgcca gcaaaacggg cggcggcctg acgtcgcttc ccgccaatga
     1321 agccacgctt tcggcgcgta tcgaaagggc aatcaaaacc tggcaaggcg aactgcccaa
     1381 aagtgagcag ggctatgtgt tcgtgctgga agatagcgag acaggcacag tggcggggat
     1441 ttgcgccatt gaggtggcag ttgggctgaa cgatccctgg tacaactatc gcgtcggcac
     1501 gctggttcac gcttcgaaag agctgaatgt ctataacgcg ttgccgacgc tgtttctcag
     1561 taacgatcac accggcagca gcgagctgtg cacgctgttt ctcgacccgg agtggcgcaa
     1621 agagggcaac ggctatttgc tgtcgaaatc gcgctttatg tttatggcgg cttttcgcga
     1681 caagtttaat gacaaagtgg ttgccgaaat gcgcggggtg attgacgaac atggctattc
     1741 accgttctgg caaagcctcg gtaagcgctt cttttcgatg gattttagcc gcgccgattt
     1801 tctctgcggc accgggcaaa aggcatttat tgcagaactg atgccgaaac atccgatcta
     1861 tacccacttt ttatctcagg aagcccagga cgtcatcggt caggtacatc cgcaaaccgc
     1921 gcctgcccgt gcggtactgg agaaagaagg ttttcgctac cgtaattata tcgacatctt
     1981 cgacggtggg ccgacgcttg agtgtgacat cgaccgcgtg cgcgccatcc gtaaaagtcg
     2041 gctggtggaa gtggcagaag ggcagcctgc gcagggcgat tttccggcct gcctggtcgc
     2101 caatgaaaat tatcaccatt tccgcgtggt gctggtgcgt accgatccgg caaccgagcg
     2161 tttgatttta accgccgcac aactggatgc cctcaaatgc cacgccgggg atcgcgttcg
     2221 tctggtgcgc ctgtgcgcag aggagaaaac agcatgactt tatggatcaa cggtgactgg
     2281 gtaacgggcc agggcgcatt gcgtgtgaag cgtaatccgg tatcgggaga ggtgttatgg
     2341 cagggcaatg atgccgatgc cgctcaggtc gggcaggctt gtcgggcagc ccgtgcggcg
     2401 tttccgcgct gggcgcgtct ctcctttggc gatcgtcagg taagagttga acgctttgcc
     2461 ggattgctgg aaagcaataa agccgaatta accgcgatta ttgccagaga aacgggtaag
     2521 ccgcgctggg aagcggcaac cgaagtgacg gcgatgatca ataaaatcgc gatatcaatt
     2581 aaggcgtatc acgttcgtac cggcgagcag cgtagcgaaa tgccggacgg cgcggcgagc
     2641 ctgcgacatc gcccgcacgg cgtgctggcg gtgtttgggc cgtataattt ccctggtcat
     2701 ttgccgaacg ggcatatcgt tccggcattg ctggcgggta acaccattat ctttaaaccc
     2761 agcgaactga caccctggag cggcgaagcg gtaatgcgtt tatggcagca ggctggcttg
     2821 ccgccgggcg tgctgaacct ggtgcagggc gggcgtgaaa cgggtcaggc gctgagtgcg
     2881 ctggaggatc tcgacggctt gctgtttacc ggtagcgcca atactggcta ccagttgcat
     2941 cgtcagcttt ccggtcagcc ggagaaaatt ctcgccctcg aaatgggcgg caataacccg
     3001 ctgattatcg atgaggtggc g
//
LOCUS       DAYPHN010000269         3000 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000269 DAYPHN010000000
VERSION     DAYPHN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.271"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1201
                     /locus_tag="ACG0BW_004688"
     CDS             <1..1201
                     /locus_tag="ACG0BW_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261536.1"
                     /translation="IWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQMLDVAWDDD
                     GNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAGGAGRRQAKV
                     ILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLSNLDAIIQDQ
                     TFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPKQAQMIITTI
                     KTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKAERLERAERQ
                     IMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDSVRRHQMEML
                     IEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESGQDQPRGNGD
                     SSTQENE"
     gene            1223..2110
                     /locus_tag="ACG0BW_004689"
     CDS             1223..2110
                     /locus_tag="ACG0BW_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFK7261537.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERNAALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            2136..>3000
                     /locus_tag="ACG0BW_004690"
     CDS             2136..>3000
                     /locus_tag="ACG0BW_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFK7261538.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREKTLSEATFIDVNDTIPEGAATFEECVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLILGDSSSNNKAFDGIPRLMHADQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIIND
                     FIPTDSGKASIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSLA
                     NKHDKAIAALTN"
BASE COUNT          839 a          652 c          791 g          718 t
ORIGIN      
        1 catatgggtt gttgtcgata gcacgatgag tggtgatgtt gagtcagtag ctgacgagaa
       61 aaagaaagat gctcgcgcct acgcctactg gatttcacct cagcaaatgc tggatgtggc
      121 atgggacgac gacgggaata tgttgtgggc gttaattgtg gaagtcgctc gtgatgacgc
      181 agatcctttt acttctacag gtcaggaata ccaacgttat cgtctgtgga cacaaaacga
      241 gtggtatctg ttccgtgagg aagtgaagaa gggcgctggt ggagcaggtc gccgtcaggc
      301 aaaagttatt ttagaggata gcggtgagca taatctcggc gtagttcctg tgtttcctgt
      361 tgattgtatt ggagaaagtg aatcaccgta tttcagccca tcgttgatcg atgatatcgc
      421 ttatcttgat cgtgcggttg caaactatct gtcaaacctt gatgccatta ttcaggatca
      481 gacatttagc cagttggcta taccggtaca gtcgctttta cctggtgatg aaaaccacac
      541 taaagtgctt gaaatgggca caaagcgagt cttcacctac gattctgaag gtggaaacca
      601 gccgttttat ctgtcaccag acccgaaaca agctcagatg atcatcacta cgattaagac
      661 ggtgattaac gaaatctacc attcagttgg tgtagctggt gagcgaacga agcaggacaa
      721 cgcacaggga atcgataatt catctggtgc cgcaaaaatg tatgacttcc agcgtgtaaa
      781 tagcttgctt gtcacaaaag cagagcgtct ggaaagggct gaacgccaaa tcatgctact
      841 ggttgcgaaa tggatggggg tagatctgga cgaagaccac tctttaattg cgtatccaga
      901 aagtttcgat attcgtggcc ttactgatga attttctgtt gccgagaaac tgtcattact
      961 tcaggcaccg gactctgtac gtcgtcacca gatggaaatg cttattgaga agattttccc
     1021 gaacattact gaggcgatga aaaaggaatt tgataaagat ctcttgaatt ttcctccaaa
     1081 aaatgatcta aatacccttg aaaataagtc agtacttact tatgatcgtg gtgcagccca
     1141 agaaagcggg caagatcaac cccgagggaa tggggactca tctactcaag agaacgagtg
     1201 ataagtaaca aaaggaattt ttatgaatct gtggcaaatg cttttggccc gtcgtggtct
     1261 gatggatgtc gctgaagcgc atgagcgtgg aggcgctggc ggtgtagctg ctgataatga
     1321 gcagagtaca caagatcctg acaaacaggg tgaacaaaaa gagcagccga agggcgatga
     1381 cgaatacgct ggcatgactc aggaagagtt actggccgaa cttcgtaaaa ccaagaaagc
     1441 tggtgctgaa ctgctgaagg agaacatgaa gcgcaaagag aaagagcgca cattggccga
     1501 tcagcttgct cagtacggtg atatcgatcc ggctcgtgct cgccagcttt tagaagctga
     1561 acaggccgca gaaaacgcac gtcgtgaggc ggagcaagct gaactggagc gtcgtggtga
     1621 gttcgatgct gttaaaaaac agatgatcga agcacaccag gcagagctgg cacagcgtga
     1681 cgaacgtaat gcagcactgg aaagtgaaaa cgcatcactg aaatctcaat tagtcgagat
     1741 gaccgtgggc gcttccttca gtaactctct cttcctacgt gacaaagttc taatgacccc
     1801 ggcaaaagcc cgcgtgatct acggttctca ttttgaagtg ggtgaagacg gtagcgtagt
     1861 gggttatgac aaaccggcag gtcataaaga acgagctgtt ctggttgacg gtgaaggtaa
     1921 gccgttgccg tttgaatccg cgattgaacg cattgtgcgg gcagatccgg aagctgacgc
     1981 attgatgcgt agcgaagcca agcagggtgt aggctcacat tccaaatcga cctacacaat
     2041 atcccaaccg aagaacaagt cgactatgga taagttggcc tccggtctgg ggaaaattgg
     2101 acttaagtaa catctaaatc aaagggaatt gatagatgcc attactgcgt gaagaagctg
     2161 aaaagctgtc taacaacgaa cttgaacagg gtgtgatcga aactatcatc gatcgcgatg
     2221 acctgtttgc catcctgcct tttatgaaaa ttaattcaaa ggcatatctg tacaaccgtg
     2281 aaaaaacgct gagtgaagct accttcatcg acgttaacga caccatccct gaaggtgcag
     2341 caaccttcga agaatgcgtt gcgaaactgc gtattctggc tggtgacgta gacgttgata
     2401 aattcctggc gaccactatg gccgacacca acaaccagtt ggctattcag gttcgtcaga
     2461 aagttaaagg tctggctcgt gcgttccgtc gcaacctgat tttgggtgac tccagctcca
     2521 acaacaaggc gtttgacggc attccacgcc tgatgcacgc agatcagaag atcgatatcg
     2581 ccggtgcatc tatgactttc tctatgttcg acgaactggt cgatgcggtg aaagatcttg
     2641 gtgctgactg catcatgatg cgttcagagc acctgcgtgc ttaccgtgcg ctgctgcgta
     2701 cagtaaacgt aggtccgtct gaaatcatga tggagaactt cggtcgtccg atgctgtgtc
     2761 ataacggcgt tccgttcatc atcaacgact tcatcccgac cgattctggc aaagcaagca
     2821 tctactgcct gcatctgtca gaagagaacg gcgttactgg tctgtatggc ggcgacaacg
     2881 caggtatcgt tgttgaaaac attggtactg tacagaacaa agacgcagta cgtacccgtg
     2941 tgaagtggta ctgctctctg gcgaataagc acgataaggc tatcgctgca ctgaccaatg
//
LOCUS       DAYPHN010000270         2919 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000270 DAYPHN010000000
VERSION     DAYPHN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.272"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..82
                     /locus_tag="ACG0BW_004691"
     CDS             <1..82
                     /locus_tag="ACG0BW_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFK7261539.1"
                     /translation="KCLSGCKFRNNVGNFGGFLSINKLSQ"
     gene            88..831
                     /locus_tag="ACG0BW_004692"
     CDS             88..831
                     /locus_tag="ACG0BW_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFK7261540.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPYLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            729..1376
                     /locus_tag="ACG0BW_004693"
     CDS             729..1376
                     /locus_tag="ACG0BW_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFK7261541.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1437..>2919
                     /locus_tag="ACG0BW_004694"
     CDS             1437..>2919
                     /locus_tag="ACG0BW_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFK7261542.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDRLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGR"
BASE COUNT          618 a          730 c          960 g          611 t
ORIGIN      
        1 caaatgcctg agcggttgta agttccgcaa taacgtcggc aactttggcg gcttcctttc
       61 cattaacaaa ctttcgcagt aaatcccatg acagaaacag aatcagcgat tctggcgcac
      121 gcccggcgat gtgcgccagc ggagtcgtgc ggcttcgtgg tgagaacgcc ggagggagaa
      181 agatattttc cctgcgtgaa tatctccggt gagccggagg agtatttccg gatgtcgccg
      241 gaggactggc tgcgggcaga aatgcagggt gagattgtgg cgctggtcca cagccacccc
      301 ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag tgatttgccg
      361 tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgta tctcaccggg
      421 cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg
      481 gcggggattg agatgccgga ttttcatcgc gaggatgact ggtggcgtca cggtcagaat
      541 ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag
      601 ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac
      661 tgtggtgacg gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat
      721 accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct
      781 gcctttacgg ggatttacaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg
      841 ctgaagccat ccgggcgctg gccacacagc ttccgtcgtt tcgccagaaa ctgaatgagg
      901 gctggtatca ggtgcgcatt tccgggcgtg atgcaggcga aaatgaatta tctgcccgtc
      961 ttaatgaacc gctggcaaat ggtgccgtga tccacgtcgt tccgcgtctg gcgggtgcca
     1021 aaagtggcgg tgtgtttcag gcggtgctgg gtgcggcgct gattgcggtg gcatggtgga
     1081 accctgttgg ctggctgggg gccgcggctg tatcgggtct gtatgcagca ggggccagta
     1141 tgatcctggg tggtgtggcg cagatgctgg caccgaaagc caggacgccc acggcagcca
     1201 gtacagataa cggcaaacag aacacctatt tctcgtcact ggataacatg gttgcccagg
     1261 gcaatgttct gccggttctg tacggtgaaa tgcgcgtggg gtcacgcgtg gtttctcagg
     1321 agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt cgctgatgca
     1381 aaatgtttta tgtgaaaccg cctccgggcg gttttatcgt ttatggagca tgacgaatgg
     1441 gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg aagtccacgc
     1501 agctgctgag tgtgatcgat gccatcagcg aagggccggt tgaaggtccg gtggatagat
     1561 taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaggggaat accaatatat
     1621 ccggcgtcac ggtggtgttc cgtgccggtg agcaggagca gacaccgccg gaggggtttg
     1681 aatcctccgg ctccgagacg gtgctcggta cagaagtgaa atatgacact ccgatcaccc
     1741 ggaccatcac gtcggcaaac attgaccgtc tgcgttttac tttcggcgtg caggcactgg
     1801 tggaaaccac ctcaaagggg gacaggaatc catcggaagt ccgcctgctg gttcagatac
     1861 aacgtaacgg tggctgggtg acggaaaaag acatcaccat taagggtaaa accacttcac
     1921 agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat atccggatgc
     1981 gcaggatgac gccggacagc accacagatc agctgcagaa caaaacgctc tggtcgtcat
     2041 acaccgaaat tatcgatgtg aaacagtgct acccgaacac ggcactggtc ggcgtgcagg
     2101 tggattcgga gcagttcggc agccagcagg tgagccgtaa ttatcatctt cgcgggcgca
     2161 ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt atctgggacg
     2221 gaacgtttaa accggcatac agcaacaaca tggcctggtg tctgtgggat atgctgaccc
     2281 atccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa tgggcgctgt
     2341 atgtcatcgg ccagaattgc gaccagtcgg tgccggatgg ctttggtggc acggagccgc
     2401 gcatcacctg taatgcctgg ctgaccacac agcgtaaggc gtgggatgtt ctcagtgatt
     2461 tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg ttcgtgcagg
     2521 accgaccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg ccggatgatg
     2581 gcgcgccgtt ccgctacagc ttcagcgccc tgaaagaccg ccataatgcc gttgaggtga
     2641 actggattga cccggataac ggctgggaga cggcaacaga gcttgtggag gacacgcagg
     2701 ccattgcccg ttacggtcgt aacgtcacga agatggatgc ttttggctgt accagccggg
     2761 ggcaggcaca ccgcgccggg ctgtggctga ttaaaacaga actgctggaa acgcagaccg
     2821 tggacttcag cgtgggtgcc gaagggcttc gccatgtgcc gggtgatgtt attgaaatct
     2881 gcgatgatga ctatgcgggg atcagcatcg gcgggcgcg
//
LOCUS       DAYPHN010000271         2910 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000271 DAYPHN010000000
VERSION     DAYPHN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.273"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            49..732
                     /locus_tag="ACG0BW_004695"
     CDS             49..732
                     /locus_tag="ACG0BW_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFK7261543.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTVINSTKTLASVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTMNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            776..>2910
                     /locus_tag="ACG0BW_004696"
     CDS             776..>2910
                     /locus_tag="ACG0BW_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFK7261544.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGVQAQAGRDNGQGVNYGQGTGTGWVAIGEDAKANSFTDTGGGSSTAVGYHSTA
                     DGRWSTALGAKTHSLGEASVALGINTTSAGERSLAIGASATSTGGFSIALGRYANSVG
                     EFSIAQGDHAETGADDAIAFGRESKALGIMSIALGATANASKEYAMALGASSAASAAN
                     AIAVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIA
                     LGSGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALG
                     QGSYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDI
                     STTSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGL
                     DNNTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNT
                     ANITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTDATSKITNVKDGDLTAGSTD
                     AVNGSQLKTTNDNVATNTTNITNLTDSVGDLKDDALLWNGTAFSAAHGTDATSKITNV
                     KDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNA
                     TAGAFSAAHSTDATSKITNVTAGDLTAGST"
BASE COUNT          836 a          703 c          743 g          628 t
ORIGIN      
        1 tgtactgtaa ttatcaacgt atcgggatta tgaaaaggaa ttcatcctat gaacctaaag
       61 aaaacactgt taagcgtgtt aataatagtg cctctctgct tattggcagg gtgtgactat
      121 attgaaaaag cgagtaaggt cgacgacctc gttacacagc aagagttgca aaaaagcaaa
      181 attgaggcgc ttgagaaaca gcaggaactc gataagcgaa agatagaaca ttttgaaaaa
      241 caacaaacta ctgtcataaa tagtaccaag acgctcgcta gcgtggtgaa agcggttaaa
      301 gacaagcagg acgaatttgt ctttacagaa tttaacccag cacaaacaca atatttcatt
      361 ttaaataacg gatcggtcgg tttggcaggc cgggtgctgg ctattgatgc ggttgaaaat
      421 ggcagtgtta ttcgtatttc gctggttaac cttttaagcg ttcccgtatc aaatattggc
      481 tttcatgcga catggggcaa tgaaagaccc accgatgcca aagctctggc aaagtggcaa
      541 caattacttt tcaacaccac catgaattcc actctgcaat taatgcctgg tcaatggcaa
      601 gacattaatc tgacattaaa aggcgtttcg cctaataacc tgaagtatct gaagttgtcc
      661 atcaatatgg ccaatctcca gttcgacact gttcaatctg ctgaaactcg gcagcgaaaa
      721 aacaaaaaat aaatgcagct tctgcgctta tatcaaggaa tagagagcat caataatgaa
      781 caaaattttt aaagttatct ggaatccggc aacaggcagt tacaccgttg ccagtgaaac
      841 ggcaaaaagc cgtggcaaga aatccgggcg cagtaagctg ttaatttctg ccctggttgc
      901 tgggggaatg ttgtcgtcgt ttggggtaca ggctcaggca ggaagggaca acggtcaggg
      961 cgtcaattat ggccagggaa caggaacagg ctgggttgct atcggtgaag atgcaaaagc
     1021 aaatagtttt acagacaccg ggggtggctc cagcaccgca gtaggttatc actcaactgc
     1081 tgacggaagg tggtcaacag ccctcggtgc taagacccat tcgcttggcg aagcatcagt
     1141 ggcgttaggg attaacacta cctcagcagg tgagcgaagc ctcgctatag gtgcatcggc
     1201 aacgtcaacg ggtggatttt caatcgcact tggtcgttat gcgaactcag taggtgaatt
     1261 ttctattgca caaggggatc acgcagagac tggtgctgat gacgccatcg catttggacg
     1321 tgaaagcaag gccttaggaa ttatgagtat cgccctcgga gccacggcta atgcgtcgaa
     1381 agaatatgca atggcgctgg gtgcaagtag cgccgcatct gcagctaacg cgattgcagt
     1441 aggccgcaac agtgctgcag ctggcgtaga cagcctcgca ttcggtagac tatccgccgc
     1501 caacgccgca aacgcgattg ctatgggtgc agagagcaaa gctgctgaaa atgcgacggc
     1561 agttggtact aacgcggagg caaatggact taatagtatc gcgttgggct ccggcagtat
     1621 tgcagatgtc gacaacacga ttgctctggg caatcaaagt caggcagtag cggctggcgc
     1681 aattgccatc ggtcaaggga ataaagctga cggcgcaaat gctatcgcgc tgggtaatgg
     1741 tagtattaca ggtggtgtaa atgctattgc tcttggacaa ggcagttatg ccggtttaga
     1801 aaatggcact gcaattggtg ctcaagccag tgctcagggg aaaaattcag ttgctctggg
     1861 tgctggttct gtagcgactg acgcggatac tgtttctgtg ggtaacacaa cagctcagcg
     1921 acaaattgtc aatatggcag caggtgatat cagcactacc agtactgatg ccatcaatgg
     1981 atcacagctt tatgctatca gtaagtcagt agcggacaat cttggtgggg gggctaccgt
     2041 caatgcgcaa ggcgtcgtta cttccccaaa ttacaggctg aaaagtggta ttttcggcac
     2101 tgttggcgac gccttaacgg gcctggacaa taatacgtta caatgggact cccttaaaaa
     2161 ggcatatagt gcggcacatg gtacagatac taccagtacc atcaccaacg ttaaagacgg
     2221 cgctatttct gataccagta aggatgcggt taacggttct cagctgaaaa ccaccaacga
     2281 taacgtagcg accaatactg ccaatatcac caccaacact aacagtatca ataccctgac
     2341 ggattccgtt ggcgacctta aagacgatgc cctgctgtgg aatggcaccg cgttcagcgc
     2401 cgcgcacggc accgatgcca ccagcaaaat caccaacgtc aaagacggtg acctgacggc
     2461 tggcagcacc gacgcggtta acggctctca gctcaaaacc actaacgata atgtggcaac
     2521 caacaccacc aacatcacca acctgacgga ttccgttggc gaccttaaag acgatgccct
     2581 gctgtggaat ggcaccgcgt tcagcgccgc gcacggtacc gatgccacca gcaaaatcac
     2641 caacgtcaaa gacggtgacc tgacggctgg tagcactgac gcggtaaacg gctctcagct
     2701 gaaaaccact aacgatgccg tggcagccaa caccaccaat atcgccacga ataccaccaa
     2761 catcaccaac ctgactgacg cagttgacag cctcggtgac gattccctgc tgtggaacgc
     2821 tacagcgggg gcattcagcg ccgcacacag tactgatgcc accagcaaaa tcaccaacgt
     2881 caccgctggc gacctgacgg ctggcagcac
//
LOCUS       DAYPHN010000272         2752 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000272 DAYPHN010000000
VERSION     DAYPHN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.274"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            243..875
                     /gene="yqeH"
                     /locus_tag="ACG0BW_004697"
     CDS             243..875
                     /gene="yqeH"
                     /locus_tag="ACG0BW_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7261545.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1209..2018
                     /gene="yqeI"
                     /locus_tag="ACG0BW_004698"
     CDS             1209..2018
                     /gene="yqeI"
                     /locus_tag="ACG0BW_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7261546.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHAINSSTDAHTHNAPPAVKGNAGYKESISGANNFNNKILKHIKSYLIML
                     SIFAIGVYSAHWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            2011..2493
                     /gene="yqeJ"
                     /locus_tag="ACG0BW_004699"
     CDS             2011..2493
                     /gene="yqeJ"
                     /locus_tag="ACG0BW_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFK7261547.1"
                     /translation="MIDYKKNLLFILVFISGFILFIVYSYTAEKMIYNETCTANWVIF
                     NDKGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(2526..2606)
                     /locus_tag="ACG0BW_004700"
     CDS             complement(2526..2606)
                     /locus_tag="ACG0BW_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261548.1"
                     /translation="MKDVDQNFDALYWHILQAYLILLFYD"
     gene            complement(2642..>2752)
                     /gene="yqeK"
                     /locus_tag="ACG0BW_004701"
     CDS             complement(2642..>2752)
                     /gene="yqeK"
                     /locus_tag="ACG0BW_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFK7261549.1"
                     /translation="VLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          962 a          468 c          492 g          830 t
ORIGIN      
        1 gatatagata ttctcatacc atgagatagt tatctgaacc accatcaacc aggtaagatt
       61 aaatcagtga aacataatca tatttaataa cgagaaatgc atttttaaat gctttactca
      121 gaattatagc aaattcagat taatcgcaat ttttcccagc aaatttacaa aatcatggtg
      181 tattgacatc atcaataacc aatgaaatta tgcaattata tacggatagg gaggttctta
      241 acatggggct ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca atgcattgcc
      301 aaataatgcc agaagagtca ccgcgtttgc agtattgcgc aaactcgtgt ttttgtatgt
      361 ggcccgaaga aagttcatat tttaatcgtg gtgtggtgga aggtatttta acaaaaaacc
      421 acaatgcgag attaagcgga tatatttttg tcgatttttc agtaagtttt ttacgtctat
      481 tccttgaaaa agactggatt gactatcttg caagtactga catggggatt gttttagtca
      541 gcgaccgaaa tatgcaatcg ttagctaact actggcgaaa acacaactct gcaatatctg
      601 ctgtcatata taatgatgat ggtcttgatg ttgccaacga aaagatcaga caactattta
      661 ttggtcgtta tttatcattt accagaggga acacattaac tcagatggaa ttcaccataa
      721 tggggtatat ggtttctggt tataatccat atcagattgc tgaagttctg gatatggata
      781 tccgtagcat ctacgcgtac aagcaacgaa tcgaaaagag aatgggtggt aaaataaacg
      841 agttatttat tcgttcacat tcggtccaac attgatactc aataatctgc cagaatcata
      901 ctctgcttat acgggcatct attgaaatgc ccgtatttta caaataagca gtaaccccaa
      961 gagtaaacaa catggtattt aaaaccatca cacgaaaaat tcagaagcat tacgaaatat
     1021 gaatttacaa acaatagtgg cataaatgtt aatcatgtta atttacgtaa agttttaggt
     1081 tgcgacatta aagcctcatt tcaatcatta tgataaatat aaaattaata tatatttatg
     1141 ccgtaaatac caatatactt agcaaactat gtgatttcca tttcgattga tttagtgtct
     1201 attgacgtat gtactggatt attaacgata atatcgagtt ctggcctgag caccgaaaat
     1261 taatatcggt acataacgcc gatcttaacg tcgttctgac aacgccagcc agtcgttgtt
     1321 tatcacttct acttgaagct tttcctgatg tggttgcaca acaagatttt ttcaccagag
     1381 tctgggaaga agaaggtatg cgtgtgccaa ctaacacgtt atatcagaac atatccatta
     1441 tcagacgcgg atttcgcgct gttggtgata ccacccactc gcttattgca accgtgccga
     1501 gaagaggatt caagattcat aatgacatta acatacaaaa tcatgcaata aactcttcaa
     1561 cagacgcaca tacacacaat gccccacctg ccgtaaaagg taatgcgggg tacaaagaga
     1621 gcattagtgg cgcaaacaat ttcaataaca aaatcctcaa acatataaaa agttatctaa
     1681 ttatgttgag catatttgct ataggcgtat attctgcaca ttggctatgg aataataatc
     1741 aaccgaagcc attcttcaag gattacaaga ctgttgcgga aattaatggc tgccatttta
     1801 atgtgacaga agatacaatt gatgggttga aagagttcga taaatataag acacggatac
     1861 tggattcggg gattaactgt aaaaaatatc cgtggttata cttccctctt gcaaaatcct
     1921 caccagggat gattgttatg gcatgcaata aaaactataa ccaacatgaa gtggcaggct
     1981 gtttaaccct gtcttaccgt gaggttaatc gtgattgatt ataaaaaaaa cttattattt
     2041 attttagttt ttatcagtgg gtttattctc ttcattgttt actcttatac tgcagaaaaa
     2101 atgatatata atgaaacatg cactgcaaat tgggtaattt ttaatgacaa ggggcgagca
     2161 aatcttacca tcgactttat gtataacaaa aaaaataaaa caggaactgt tgcgttgagc
     2221 ggaacctggc aacagggtaa cagagaaagt aagtcaatac ggcgaaatat tgaatacaca
     2281 tggattgaaa actatgacac agcccattta acatcaaaga aagttaataa atttgagatc
     2341 atggatcaag tcgatgatga tagacttgca gaacttattc ctgattttta tgtctttcca
     2401 gaaaaaagtg taagctataa tatactaaag caaggtaagc atgcttttat tttgagtatt
     2461 ggtaacagag caataatgca ttgtgcaagg taaacttaaa tagataacaa aaagatggaa
     2521 cctcgttaat cataaaacaa taaaattaaa tatgcttgta gtatatgcca gtataaagca
     2581 tcaaagtttt gatcaacatc tttcatttta gatatcacct tgcaatgtaa aatatcatgt
     2641 atcaacaaca tctggtgcaa atccatgagt cgaactcaac attcatctta gctctattat
     2701 tattccttcg tacagtcgat gtaaacaata cagaaagagg attattaaga ac
//
LOCUS       DAYPHN010000273         2737 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000273 DAYPHN010000000
VERSION     DAYPHN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.276"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..542)
                     /locus_tag="ACG0BW_004702"
     CDS             complement(<1..542)
                     /locus_tag="ACG0BW_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFK7261550.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEHGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGSASTKAT"
     gene            complement(607..1206)
                     /locus_tag="ACG0BW_004703"
     CDS             complement(607..1206)
                     /locus_tag="ACG0BW_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFK7261551.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(1276..>2737)
                     /locus_tag="ACG0BW_004704"
     CDS             complement(1276..>2737)
                     /locus_tag="ACG0BW_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFK7261552.1"
                     /translation="FLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAV
                     NAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRI
                     TDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDA
                     EGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQ
                     TKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMND
                     VFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTINQNCTIK
                     GMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIF
                     NGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGS
                     VTLEFKVFHKGNQWAGNITDCTVIVTKKAASGISIR"
BASE COUNT          592 a          805 c          664 g          676 t
ORIGIN      
        1 gtagcctttg ttgatgccga tcctgcgctg gaagacgctg actgagccga cgacgcggcc
       61 tgtccggctg acgtgctggc tgcgcgtgct gagcctgcag catcagtcgc atgggttgcc
      121 gcctcacggg ctgatgtgct ggcatcgctg gctgacttct tcgcggctgc cgtgttctgt
      181 gccaccgcgg acgcgttacg cgccacctct tccaccatca gctcaaaacg gcgcagtgcc
      241 tccggacggg catcatcctc cgtcatggca ccgagaaaat cattcagcgt accgggtcgg
      301 gaatcttcat acacggtaat ggtcccggca tgtgatggcg ggaatccctc caccaacaga
      361 ataacgctgt actgaccgtg ctcaacgtcc atgctgtaac gcccggcttc atccggattt
      421 tctgaggcca gcgtgttcac caccaccgtg gtgctgttac gttttgcttt cagctggatt
      481 gtgcagttct gtaccggttt tcctgtgccg tctttcagta cacctgaaat ctttactgcc
      541 atattcaccc cacaaaaaat cccgcctgaa acggcgggct gtcataacac tgtgttacct
      601 ggctaatcag aacttataac cgacacccac gatgaaaccg tcagtgcgcc agtcaccact
      661 gccggaacct tcataagcaa tatcaatggc cacggattcg gtcgggttaa actgcacgcc
      721 agccccccac gccagagacg tgttgctgtg gcgaccgtca tcacttccgg tcagcacatc
      781 atgcgttttc cccttgttgt cagttacgcg gagataatcc ccggaaaaag tcgacacacg
      841 gctgtaagcc acgcccgcca tcgcatacgc actgaaccat tcattcacgc gcacagaggg
      901 ccccgccatc acgctgaacc agcggttacg cacggaatct tcatgccagc gggtatcgct
      961 gtaacgggta agctggcgat tcttgtctcc tgcatagctg aatgacgtca ccagccccag
     1021 tgtgtccgta aactcataac ggtatttcac gttaatcccg ttcagatcat cgctgccggg
     1081 aacgttcgtc gaggcatgaa gataccccgc gctcagcgtg gactgatgtt cagacgccca
     1141 tgcaggcgca ccggatacgg ccagacaaat ggctgcggac aaaatggcgg cataaagttt
     1201 acgcataatt acctctcgct tttctgcaat aaaaaaggca ccatttctgg tgcccttata
     1261 tgggttataa caatttcaac gaatactgat gccggaagcg gcttttttgg tcacaatcac
     1321 cgtacagtcg gtgatattac ctgcccactg attgccttta tggaaaacct taaactccag
     1381 agtgacgctt cccctgccac tcggcatatc aataaccgca ctgtagctac cgggaatggc
     1441 ccctttagtt tctctggatg cgattaatac accgtttttg cgaacttcaa aaccataacc
     1501 cgtgtatctt gtacctcccg ggttattacc acttcccgga tcgctatacg ctattccgtt
     1561 aaagataatg ggcggaataa tgatttgacg gtcaaagtta tgatcatcgc tgatggtgac
     1621 tgtaaccgtc ccgtttggtg tttccgtgtt accccacgta ccagcctgtt tcggaaatga
     1681 tttggataca gctttaacga agtcacctct gacctgagtc gcctccagca tgcccttaat
     1741 cgtacagttc tggttaatcg tgacattgtt gagcgttcct gagttcgcat tcacactacc
     1801 actgatatcc gcatttttag cggtcagctt tccgtctgat gtcagggaaa ataccggagg
     1861 actgccaccg ctggtaatgg tgggggctgt caggcgcttc aggaacacgt cgttcatgaa
     1921 tatctggttg ccctgcgcca caaacatcgg cgtttcattc ccgtttgccg ggtcaataaa
     1981 cgcgatacgg ttagcggcaa ccagaaactg gctcagcttg ccttcctccg tgtcctccat
     2041 gctgaggcca atacccgcga cataatgttt gccgtctttg gtctgctcaa ttttgacgcc
     2101 ccacatggca ttccatttat cgttagcgtc cttccactct ttcgaaaact catccagtct
     2161 gctggcgtta tcctccgtca gatcaacttt ttccagcagc tctttaccga gatgggattc
     2221 ggttatcttg cctttgaaaa aatccaggta accttccgca tcatcgctcg cccgaccgac
     2281 ggcctccacg aatgccgatt tgccaacggt gttcacactg cggatataaa agtaataatc
     2341 atggcccggt ttgatattga tactggcggc tatccagtac agcgccgtac caagatagcg
     2401 ggctgtggtt tcaacctgcc tgatatcggt aatccgcttt tccgagaacc agaactcaaa
     2461 ctgtaccgtc gggtcataaa cggcaagatg cggcgtggcg gttatctgaa aatagcccgg
     2521 cgtcagctca atcctcgacg gtgctgccgg tgcggcaatc cggaacgata ccgacgccgg
     2581 atcgccctgc tgcccccacg catttaccgc ccggactgtc agcctgtagt tccccagcgc
     2641 cagttgcgtg aagcggtatg tggtttccgt cgtccgggcc gtgctgacca gccgctcact
     2701 gccgtcatcc gctgttacgg tcagacggag caggaag
//
LOCUS       DAYPHN010000274         2663 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000274 DAYPHN010000000
VERSION     DAYPHN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.277"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..231
                     /locus_tag="ACG0BW_004705"
     CDS             <1..231
                     /locus_tag="ACG0BW_004705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261553.1"
                     /translation="NKNREHYMLYKIFTLNTKIKTTIITICALSYNLTAQASPSYRPR
                     DRVNKTMIELQTNGVERQLLDNSCGISSGVFK"
     gene            complement(249..584)
                     /locus_tag="ACG0BW_004706"
     CDS             complement(249..584)
                     /locus_tag="ACG0BW_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFK7261554.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFTDTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            713..1060
                     /locus_tag="ACG0BW_004707"
     CDS             713..1060
                     /locus_tag="ACG0BW_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFK7261555.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1080..1589)
                     /locus_tag="ACG0BW_004708"
     CDS             complement(1080..1589)
                     /locus_tag="ACG0BW_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261556.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLPAGGTSATDLAV
                     ELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGMEVR
                     VQINESAAP"
     gene            complement(1586..1846)
                     /locus_tag="ACG0BW_004709"
     CDS             complement(1586..1846)
                     /locus_tag="ACG0BW_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261557.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            1948..2663
                     /locus_tag="ACG0BW_004710"
                     /pseudo
     CDS             1948..2663
                     /locus_tag="ACG0BW_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2229..2345
                     /locus_tag="ACG0BW_004710"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          671 a          681 c          667 g          644 t
ORIGIN      
        1 aataaaaata gggaacatta tatgctttat aaaattttca cattaaacac aaagataaaa
       61 acaactatca ttacaatatg tgcattatca tataatctta cagcgcaagc ttccccgtca
      121 tacagacctc gtgatcgagt taataagact atgatagaac tacaaactaa cggagtagaa
      181 cggcaacttt tagataattc ttgtggcata agttctgggg tatttaaata aaatataaga
      241 caggctgtct atcttacaga cagccttttt ataataacag aaagaatata tatcatactt
      301 aacggaagag ataatatgaa aacagtaccc gtataaacag caagcaatcc catttttcca
      361 ggtgtatcag taaaaaaacc gctgttccag aaatcaggcc gggtaaattt cagagctgta
      421 tcttcaatat accattttgc aaccggatac aaaaccattc cgcagagaga aatacaccag
      481 aacagtaatc tgtatttaaa atcataatcc catgacatat acagcatata tccccccgtc
      541 acccatcccc accacatatt attaaaataa tattttctgt tcatctgcca gttttccttt
      601 cttttgcact tttgtcagtg tactgatgca tgacaacata accacagcat gataataata
      661 atcaataaca ataagctgtg tcacgtttac atcatggaac ggaggtgctc ctgtgacttt
      721 tgagcagaaa aaagcccggg ccattgccct gatggacagc aaaaagatgt ggcggagcaa
      781 ttatgcccct cctcttctgc gtattctctg gcggctgggt atccggcttc cgccattacc
      841 ctttatgccg ttctggcagg tcacggtgct gacggggggc ttatggggca tttcctgggg
      901 atgtgcaatg tggtttatct actgggggcc gtcaggaatg gtggcaggtg aggccattat
      961 tataagtatc acgggggggt ttttgtccgg actgctcatg gcctctttcc actggtggcg
     1021 caggaaagtt aaccggctgc caccatggga tgatgtgtaa accgtgcaca ggcgtgttct
     1081 tacggagcgg cactttcatt tatctgtacg cgaacctcca ttcctgcatc gtgacaggcc
     1141 tgcagccact gcgccacttc cagcggatcg ccctcccggc gtaccaccct gccttcttta
     1201 ttccataact gcagacaggt gctgccgtcg agacgcacca caaaatcccc acggcaggcc
     1261 tgatacgtta tgccgttcag ctccaccgcc agatcagtgg cagaagtgcc gcctgcgggc
     1321 agcagaggct gtgccacggc ccggccggcg tccgtcagct ccactgccag ctgcaggttc
     1381 ggagcgcgca gggtacgcag ccagccggca tcctccagac gacggcagga ggcgcgcagg
     1441 tttggcccgt agaccggggc ttccccgctt tgctccagca cccgttctat gtcccgcgtc
     1501 agcacgggtc ctggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc
     1561 agtggcgacg gtcggcggct ctgagtcatt tccagtcatc ctccggccag atgagctttc
     1621 tgaggcgctg atggtcggcg tcaatgatct cctgtctctt tatggcattc attgaggtat
     1681 agccatttat ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc
     1741 agagataagt tacgccgtgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc
     1801 gggtcagttg ttcatagctg agtgaaaacg tcatttcctg atccatcctg tacgttccat
     1861 ctgataatgt tctgacaggt aaaaatcata tcctgaaccg ccccgggaat cctggagact
     1921 aaactccctg agaaagaggt aaacaggatg actaaaaata ctcgtttttc ccccgaagtc
     1981 cgtcagaggg cgattcgtat ggttctggaa agtcagggcg aatatgactc acagtgggcg
     2041 gcaatttgtt ccattgcccc aaagattggc tgtacaccgg agactctgcg tgtctgggta
     2101 cgccagcatg agcgggatac cggaggcggt gatggcgggc tcaccaccgc tgaacgtcag
     2161 cgtctgaaag agctggaacg tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc
     2221 caggcttccg cttattttgc gaaggcggag ttcgaccgcc tctggaaaaa gtgatgccac
     2281 tgctggataa gctgcgtaag ctgtacgggg tcggaccggt atgcagtgaa ctacatattg
     2341 ccccgtcaac gtattaccac tgtcagcaac agcgacatca tcctgataaa cgcagtgccc
     2401 gtgcgcagcg cgatgactgg ctgaagaaag agatactgcg cgtatacgat gggaatcatc
     2461 aggtatacgg tgtgcgtaaa gtctggcgtc agttgttacg ggaaggaatc agggtagcca
     2521 gatgtacagt ggcgcgcctc atggcggtta tgggacttgc cggtgttctc cggggtaaaa
     2581 aggtccgcac taccgtcagc cggaaagccg ttgccgcagg cgaccgcgta aaccgtcagt
     2641 ttgtggcaga acgttctgac cag
//
LOCUS       DAYPHN010000275         2627 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000275 DAYPHN010000000
VERSION     DAYPHN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.279"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            3..509
                     /locus_tag="ACG0BW_004711"
     CDS             3..509
                     /locus_tag="ACG0BW_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFK7261558.1"
                     /translation="MTCISQPKKTTLRDKAMVNYVFDYLSSPGSLPFTTAATELSAIH
                     GHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKP
                     DTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAER
                     LLARLPAH"
     gene            complement(519..1946)
                     /locus_tag="ACG0BW_004712"
     CDS             complement(519..1946)
                     /locus_tag="ACG0BW_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFK7261559.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEDALRIAQSPAKSVWQSEEHEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDANL
                     HRNLLRWYSTSRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLIPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1943..2626)
                     /locus_tag="ACG0BW_004713"
     CDS             complement(1943..2626)
                     /locus_tag="ACG0BW_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFK7261560.1"
                     /translation="MFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSSWGIDDRDD
                     LLRTIHRMIDNGHAARLAGFYHCWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECCG
                     EGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGYTFG
                     RQYWQSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQPLADYPTCP
                     ETLKDMSDL"
BASE COUNT          750 a          631 c          588 g          658 t
ORIGIN      
        1 caattacctg tattagccaa cccaagaaaa cgacgttgcg tgataaagcc atggtgaatt
       61 atgtctttga ttatttaagc tcaccgggca gtcttccatt caccacggca gccacggagc
      121 tttccgcgat tcatggtcac tcaacgtcgc aatatcgcct tggagaattt tatcttcatg
      181 gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag caatcagcgg
      241 aacaggaaaa tccacgcgcg caaagtaaac tggggtggat ctacctcaaa ggtctggggg
      301 ttaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa caagggtatg
      361 ctcatgctca atatacttta ggtttgatct acagaaacgg ctcaggtatt aatgttaacc
      421 attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa
      481 gattacttgc caggcttccc gcacattaac gtcgactgtc acccgaacag atggagtatt
      541 ttccctatca aaccagcaaa gataaaaata tagaaaataa ttttaatgat gcccagacag
      601 cctggtccac catcgtcaac gggcattggc tgcggttgcg gctccggttc gacggtttgc
      661 ttctcggtaa aatttgccgc ctgaattaat aaccgtcgaa taattaatgc ttccagagga
      721 caacgatctt ctgcatcaag gcggctcaca atcgggatta atgattcttc tgtaggttgg
      781 ctggcctgca ctaattgttt ggcaactaga tataatggcc ataagggatg ttctgtatca
      841 cgcgtgagtg agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagagaacgc
      901 cccagcgcag agagtttttc atcactgagt atttcgtata aatgaaaacg cgtacgagcg
      961 ctgtcaccag ggacataaag tagcggtttt ccctgatcgt taaccagatc acgcgcttca
     1021 atcatcatca ccactaatgg gtaataatcg acccgatgct gacggcacca ttgcaacatt
     1081 aaagcatccg cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccagcagg
     1141 ctttcgcctt ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta
     1201 tcaggttcag cttcccgcca cgaccgggcg taatccagaa gttcggggat atccaggcgg
     1261 cttgtgctat accagcggag taaatttctg tgcaaatttg catcatcggg gataatcact
     1321 gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg
     1381 aacatttggt aaaaatcaat ggtgtcattt tgtacggcaa cgggcagatg cagaatggtc
     1441 tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg
     1501 tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg
     1561 cactcgaagg ataaatgggc agtgttcatg gctattgtgc acagcgacca gcgtaattca
     1621 tcaatatctt ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcagagggc
     1681 agaaaacgtt cgctatcaga ggcgagtaac gcacgaaagg cgagcagaat ttcatgttct
     1741 gcaacttcat gttcttctga ttgccaaaca gatttagcag gggactgcgc aatccgtagc
     1801 gcatcctcat acgcctgacg aagttgttta aaaccttgcg gatcggtttc tggatgaaag
     1861 gacggcaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt
     1921 tcgagaatct tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg
     1981 tggggtagtc agccagtggt tgccagggta aggtcgcgta atatgcatcg tcactggcaa
     2041 ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgcc aagaattcac
     2101 gtaataactg aagattatca tcttcgggag actgccaata ttgccgacca aaggtataac
     2161 cggcaaaata ttgccgccag ttactataat aacgtagagc ccgtagatga atgcgcgact
     2221 gtatccacaa gctttcttct tcactcaacc agttattaag cacgcccatt cggcttaaaa
     2281 aacccatccg gacataatcc caggctttaa ttcctccctc accacagcat tcggcggtgc
     2341 tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc
     2401 attcacatgg cgaataacga aaccagcagt gataaaaccc tgccaaccgc gcggcatgac
     2461 cgttatcaat cattcggtga attgtgcgca gtagatcatc acggtcatca attccccagg
     2521 aggaagaaag gtcaaaaaag cgtgtaccta aatacatttt gggggatgaa tgcgaaggta
     2581 aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaat
//
LOCUS       DAYPHN010000276         2533 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000276 DAYPHN010000000
VERSION     DAYPHN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.280"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            238..633
                     /gene="rpsF"
                     /locus_tag="ACG0BW_004714"
     CDS             238..633
                     /gene="rpsF"
                     /locus_tag="ACG0BW_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFK7261561.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETTDDAEAGDSEE"
     gene            640..954
                     /gene="priB"
                     /locus_tag="ACG0BW_004715"
     CDS             640..954
                     /gene="priB"
                     /locus_tag="ACG0BW_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFK7261562.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            959..1186
                     /gene="rpsR"
                     /locus_tag="ACG0BW_004716"
     CDS             959..1186
                     /gene="rpsR"
                     /locus_tag="ACG0BW_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFK7261563.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            1228..1677
                     /gene="rplI"
                     /locus_tag="ACG0BW_004717"
     CDS             1228..1677
                     /gene="rplI"
                     /locus_tag="ACG0BW_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFK7261564.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1748..>2533)
                     /locus_tag="ACG0BW_004718"
     CDS             complement(1748..>2533)
                     /locus_tag="ACG0BW_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFK7261565.1"
                     /translation="PTTIYSFPAYSPRFSSTDKPVKLKFHQYARATLLSNRGRDHNCD
                     GRHTVEIHKLDLSDWQAFNKLAMACNAYDGITMNGDNSFGWNHEATLDNIHAQKYNKA
                     YAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMP
                     NCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSSAAI
                     YSGLGFERVPQDRDIRCDHMHLTPANHPELWTQENGEWIYLRN"
BASE COUNT          666 a          579 c          631 g          657 t
ORIGIN      
        1 accatttctg atgcagttgt tcaaaaaaac accatgatga agtgtgatga acttcaaatc
       61 agcgtgttag tacgatttgg tgaaagatga gatttatttg tcggctggcc ttgagtttag
      121 cggggtatgt cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
      181 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
      241 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
      301 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
      361 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
      421 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
      481 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
      541 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccact
      601 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
      661 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
      721 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gttttcaccg gcaggcgtgg
      781 tgccaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
      841 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
      901 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
      961 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
     1021 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
     1081 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
     1141 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
     1201 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
     1261 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
     1321 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
     1381 gctgaactgg aagctaaact ggctgaagtt ctggcagcag ctaatgctcg cgctgagaaa
     1441 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
     1501 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
     1561 aagagcgaag ttcgcctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
     1621 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
     1681 gttattcaac gagacgtaaa aacgccgacc agtggtcggc gttttgcttt ctatttttcg
     1741 tcaggtatta gtttcgtaag tagatccatt caccattctc ttgtgtccat aattcaggat
     1801 ggtttgctgg cgttagatgc atatgatcgc accttatgtc tcggtcctgc ggcactcgct
     1861 cgaaacccaa acccgagtag attgctgctg aacttgataa ggaacgcaga ccaattttca
     1921 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
     1981 tagaaacgcc tgtaacagtg cgaagttggt cgatataaag atcgattgtt ggtagttcag
     2041 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
     2101 tatagtctgg agtaccataa cctggaccca aaacaacctc gtgtattgtg tatttactgt
     2161 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
     2221 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagcc tcatggttcc
     2281 atccaaaaga attatcacca ttcattgtta ttccatcgta agcattacac gccatcgcta
     2341 gtttattgaa tgcttgccag tctgagagat ctagtttgtg gatctcaaca gtatgtctgc
     2401 catcgcaatt atgatctctt ccacgatttg aaagtaatgt agcgcgtgcg tattgatgaa
     2461 attttaattt tactggtttg tcggtggacg aaaaacgggg tgaatatgca ggaaatgaat
     2521 aaatagtggt tgg
//
LOCUS       DAYPHN010000277         2484 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000277 DAYPHN010000000
VERSION     DAYPHN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.281"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            141..1541
                     /gene="xanQ"
                     /locus_tag="ACG0BW_004719"
     CDS             141..1541
                     /gene="xanQ"
                     /locus_tag="ACG0BW_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFK7261566.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            1559..>2484
                     /gene="guaD"
                     /locus_tag="ACG0BW_004720"
     CDS             1559..>2484
                     /gene="guaD"
                     /locus_tag="ACG0BW_004720"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFK7261567.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAKHLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGL"
BASE COUNT          575 a          588 c          613 g          708 t
ORIGIN      
        1 ctgtttctct gtttgaacga cgaccaaata gtgactgcga aaatgagata agccgttctc
       61 aaggcgtcct gcgccccaaa ttcgtgggaa tttattttac tgattcagat gatcaaattt
      121 actttaaaag gagtgaaggg atgtctgata taaaccatgc aggttctgac cttatatttg
      181 aactggagga tcgccctccc tttcatcagg ctctcgttgg tgccattacc catctgttgg
      241 caattttcgt tccgatggta acccccgcgt taatcgtggg tgcggcctta cagctttccg
      301 ctgaaacaac tgcctatctt gtttcaatgg cgatgatcgc ctctggtatt ggtacctggt
      361 tacaagtaaa ccgctatggc atcgtcggtt ctggcctact ctcaattcag tcagtcaatt
      421 tttcatttgt tacggtcatg attgcgctgg gcagcagcat gaaaagcgac ggttttcacg
      481 aagagttaat catgtcgtcg ctcctcggcg tctccttcgt tggcgcattt ctggttgtcg
      541 gctcttcttt tatcctgccc tatttacgtc gggttattac gcctaccgtc agcggcattg
      601 tggtgctgat gatcggctta agcctgatta aagtcggcat tatcgatttt ggtggaggat
      661 ttgcagccaa aagcagcggt acattcggca attacgaaca tctcggcgtt ggtttattgg
      721 ttttgattgt ggtgatcggc tttaactgct gtcgcagtcc gttgctacgc atgggaggga
      781 tcgccattgg gctatgtgtc ggctatatcg catcgttatg cctgggcatg gtggatttca
      841 gcagtatgcg caatttgccg ttaatcacca tcccacatcc gttcaaatac ggctttagtt
      901 ttagcttcca tcagttcctg gtggttggca cgatttatct gcttagcgtg ctggaagctg
      961 tcggcgatat caccgccacg gcaatggttt cccgccgccc cattcagggg gaagagtatc
     1021 agtcccgact gaaaggcggc gtgctggcag acggtctggt ttctgttatc gcctccgctg
     1081 tcggatcatt acccttaacc acatttgcgc aaaataatgg ggttattcag atgactggcg
     1141 tcgcttcacg ttatgtcggg cgaaccatcg cggtaatgct ggttatcctc ggcttatttc
     1201 cgatgattgg cggcttcttc acgaccattc cctcggctgt tctgggaggc gcaatgacgt
     1261 tgatgttttc catgattgct atcgctggga ttcgcatcat catcaccaac ggtttaaagc
     1321 gccgagaaac acttattgtc gccacttctt taggtttagg gcttggcgtc tcctacgatc
     1381 ccgaaatttt taaaatattg ccagcctcta tttatgtact agttgaaaac cctatttgtg
     1441 ctggcgggtt aactgcgatt ttattaaata ttatcctccc tggtggctac cgacaggaaa
     1501 acgttctgcc tggtattacc tcagcggaag agatggatta acagtaaagg agtcaatgat
     1561 gtcaggagaa cacacgttaa aagcggtacg aggcagtttt attgatgtca cccgtacagt
     1621 cgataacccg gaagagattg cctctgcgct gcggtttatt gaggatggtt tattactcat
     1681 taaacaagga aaagtggaat ggtttggcga atgggaagac ggaaagcatc aaattcctga
     1741 cactattcgc gtgcgcgact atcgcggcaa actgatagta ccgggctttg tcgatacaca
     1801 tatccattat ccgcaaagtg aaatggtggg ggcctatggt gaacaattgc tggagtggtt
     1861 gaataaacac accttcccta ctgaacgtcg ttatgaggat ttagagtacg cccgcgaaat
     1921 gtcggcgttc ttcatcaagc agcttttacg taacggaacc accacggcgc tggtgtttgg
     1981 cactgttcat ccgcaatccg ttgatgcgct gtttgaagcc gccagccata tcaatatgcg
     2041 tatgattgcc ggtaaggtga tgatggaccg caacgcaccg gattatctgc tcgacactgc
     2101 cgaaagcagc tatcaccaaa gcaaagaact gatcgagcgc tggcacaaaa atggtcgtct
     2161 gttgtatgcg attacgccac gcttcgcccc tacctcatct cctgaacaga tggcgatggc
     2221 gaaacacctg aaagaagaat atccggatac gtgggtacat acccatctct gtgaaaacaa
     2281 agatgaaatt gcctgggtga aatcgcttta tcctgaccat gatggttatc tggatgttta
     2341 ccatcagtac ggcctgaccg gtaaaaactg tgtctttgct cactgcgtcc atctcgaaga
     2401 aaaagagtgg gatcgtctta gcgaaaccaa atccagcatt gctttctgtc cgacctccaa
     2461 cctttacctc ggcagcggct tatt
//
LOCUS       DAYPHN010000278         2457 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000278 DAYPHN010000000
VERSION     DAYPHN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.282"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            85..144
                     /gene="ibsA"
                     /locus_tag="ACG0BW_004721"
     CDS             85..144
                     /gene="ibsA"
                     /locus_tag="ACG0BW_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFK7261568.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            365..1024
                     /gene="yegL"
                     /locus_tag="ACG0BW_004722"
     CDS             365..1024
                     /gene="yegL"
                     /locus_tag="ACG0BW_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFK7261569.1"
                     /translation="MSEQIMFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            1021..1782
                     /gene="pphC"
                     /locus_tag="ACG0BW_004723"
     CDS             1021..1782
                     /gene="pphC"
                     /locus_tag="ACG0BW_004723"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFK7261570.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWTE"
     gene            complement(1847..2308)
                     /gene="yegJ"
                     /locus_tag="ACG0BW_004724"
     CDS             complement(1847..2308)
                     /gene="yegJ"
                     /locus_tag="ACG0BW_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFK7261571.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIQHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
BASE COUNT          596 a          572 c          608 g          681 t
ORIGIN      
        1 aaggaggtaa gccgacgatt tcagcgggac gctgaaacgg gaaaccctct cccggagaag
       61 agggctttta ataaggaaag ggttatgatg aagcacgtca tcatactggt gatactctta
      121 gtgattagct tccaggctta ctaagaacac cagggggagg gggaaacctc tccctaaccc
      181 tcactcctga aattatgtca ctccgccctt agtgtcaatc gacggctctc cgttcttgcg
      241 aggcgaatag caaaattttc tgcgccgccg ggcctaattc tgaaagtttc ttcgtttgct
      301 cttatttcat tattgcaatc ggtatgatac cggacctcaa taattctcta agaaaggatt
      361 tcttatgagc gaacaaatca tgtttgccac cagcgatttt gccagtaacc cggaaccacg
      421 ttgcccctgc attttgctgc tggatgtttc tggttctatg aacggtagac ctattaatga
      481 acttaacgcc ggattggtta cctttcgcga tgagctgctt gccgattcgc tggccttaaa
      541 aagagtggaa ctcggcattg tgacgttcgg cccggtgcat gtggaacaac cctttaccag
      601 cgccgctaat tttttcccgc ccatcctgtt tgcccaaggc gatacaccaa tgggggccgc
      661 cattaccaaa gccctggata tggttgagga gcgcaaacgt gagtatcgcg ccaatggtat
      721 ttcctattat cgtccgtgga ttttcctgat cactgatggc gcaccaaccg atgagtggca
      781 ggctgccgcc aacaaagtgt ttcaggggga agaagataag aagtttgcct tcttttccat
      841 cggtgttcag ggggcggata tgaagacttt ggcacaaatc agcgttcgtc agcctttgcc
      901 actgcaggga ttacaattcc gtgaactgtt tagctggtta tccagttcgc tgcgttcggt
      961 ttcccgctcc acgccgggaa cggaagttgt gctggaagcg ccaaaaggct ggacgtcagt
     1021 gtgagctggc gtctggtcta tgcatcaacc gttggaacgt cgcacatcag cgcggacttg
     1081 ccttgccagg atgcctgcca gatgcaggtt gcctggttaa atgaccagca gccattattg
     1141 tcggtatttg tcgctgatgg cgcaggcagt gtctcgcagg gtggcgaagg cgcgatgctc
     1201 gccgtcaatg aagcgatggc ttatatgtcg caaaaagtgc agggcgggga attggggctt
     1261 aatgatgtcc tcgccacgga tattgtgctg accattcgtc agcgactttt tgccgaggcg
     1321 gaagccaaag aattagcggt tcgcgatttt gcctgcacat ttctgggact gatttcatcg
     1381 cctgacggca ccttgattat gcagattggc gatggcggcg tggtggtaga tttggggcac
     1441 ggtctgcaac tgccgctcac gccgatggtg ggtgaatatg ccaacatgac gcacttcatt
     1501 accgatgaag atgcggtttc cagactggaa actttcacca gcactgagcg tgcgcacaaa
     1561 gttgcagcat ttacggatgg tatccagcgg ctggcgttaa atatgctgga taattctccc
     1621 catgtgcctt tctttacccc gtttttcaat gggctggcgt cagcaacgca ggaacaactc
     1681 gatttattac ctgaattgtt aaagcagttt ttgtccggcc cggcggtaaa cgagcgtacc
     1741 gatgatgata aaacgttggc actggcgctg tggacagaat gaacatcgca tggcggttga
     1801 agacaacagt tcaaccgcca gtttaagcaa ttaatataat ggctggttag cgaactaatt
     1861 ttgctcttag ctcgtcggct tcttcttttg gataggtgac caataaagga tcaatggtat
     1921 aaccgccgta aattttatcc ccgcgcgtat acatccagtc tgaaatatct tctttcttga
     1981 ttttccactc ctgaccatat tctacgtttg taacaatacc agggtcattg cttattacgc
     2041 caataaacat gccgttagaa taagtgacat ccgtaagcca gaaatgctct gttcttccgt
     2101 gatcggttat cggggctttg accgcgaaag tatcggcatc ttttttattc ataacctcga
     2161 tgaatttatc agtttctttc cgcgcatgtt gaatggcgtc agacatcgct tgtttatcat
     2221 aacctcccgt cactaacgta tccgaaactt cactaaagcc cgcggtagta aaaaatagca
     2281 gactcaacat aagtaatatc tttttcattt tttctccaga atccctgtgt agtaaaagtt
     2341 agcaaaaaaa ctgtggaagt gaatcattat caggttggaa ttgaattgta tcctgatgaa
     2401 attgcattat catttttatt cgtgagtggg actcttccaa tatgggaata attataa
//
LOCUS       DAYPHN010000279         2457 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000279 DAYPHN010000000
VERSION     DAYPHN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.283"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..1815)
                     /gene="tssI"
                     /locus_tag="ACG0BW_004725"
     CDS             complement(<1..1815)
                     /gene="tssI"
                     /locus_tag="ACG0BW_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFK7261572.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGAVAQRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRQNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLSRL
                     WAEEGLFFFERFAADSPEQKLTLCDDVAGLSQAGEFPFNPDTSAGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGENLNGQLEQYEIFDYPGRYKDEQHGKDFTLY
                     QMESLRSDAEKATGQSNSPKLWPGTRFMLTGHPQKMLNREWQVVQSILSGNQPQALHG
                     SQGKGTTLGNQLEVIPADRTWRPRLQNKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVK
                     FHWDRYHGMTEASSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRT
                     YHEDNRSPGDLPGTKTQMTIRSKTYKGSGFNELRFEDATSNEQVYIHAQKNMDTEVLN
                     DRTTDVKHDHTETIGNDQKITVVKGQTVQVGTRKEGGHDQSITVANDRCITVRNDQTL
                     QVTNDRTVSVSNDDGLYVRNDRKVTVEGKQEHKTTGNHI"
     gene            complement(1834..2118)
                     /locus_tag="ACG0BW_004726"
     CDS             complement(1834..2118)
                     /locus_tag="ACG0BW_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFK7261573.1"
                     /translation="MPLAAKLTDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARSGDAVDCGGVVIGGGTVNIG"
BASE COUNT          508 a          778 c          564 g          607 t
ORIGIN      
        1 gatatggttc cccgtcgtct tatgctcctg cttcccttcc accgtcactt tacggtcatt
       61 tctgacgtac aggccgtcat cattactcac gctcaccgtc cggtcattcg tcacctgcag
      121 cgtctggtca ttacgtaccg tgatgcaccg gtcgttcgcc accgtgatac tctggtcgtg
      181 gcccccttct tttctggtcc cgacctgaac cgtctgcccc ttcaccaccg ttatcttctg
      241 gtcgttcccg atggtttccg tatggtcatg tttcacatcc gttgtccggt cgttgagcac
      301 ctccgtatcc atgttcttct gggcatggat atacacctgc tcattactgg tggcgtcttc
      361 aaaccgcagc tcgttaaatc cgcttccttt ataggttttt gaccggatgg tcatctgcgt
      421 cttcgttccc ggcaggtcgc ccggcgaacg gttgtcctca tggtacgtcc gccccatgat
      481 tatcggctgg tccgggtcgc cgttcaggaa gtccactatc acctcctgac ccacccgtgg
      541 tatcgccagg ttaccgaacc ccggccccgc ccacgcctgc gatacccgca cccagcatga
      601 actcgcctcc gtcatcccgt gataacggtc ccagtggaac ttcacccgta cccgtccatg
      661 ttcatcgcag aagatttcct ctcccgcagg accggtgacg atggcgctct gtggcccgtc
      721 caccttcggt ttgttttgca gccgtggacg ccacgtccgg tccgccggta tcacctctaa
      781 ctgattgccc agcgtggttc cctttccctg gctgccgtgc agcgcctgcg gctggtttcc
      841 tgacagtatg ctctgtacca cctgccattc acggttcagc atcttctgcg gatgccccgt
      901 cagcataaac cgcgttcccg gccacagctt cggcgaatta ctctgccctg tggccttttc
      961 cgcatcactg cgcaggctct ccatctggta cagcgtgaaa tccttgccgt gctgttcatc
     1021 cttgtacctt cccggataat caaatatctc atactgctcc agctgtccgt tcaggttctc
     1081 accctgctgc tcatacatcc ccggccagtc cggcacctta aaggtgtaat cctggctctg
     1141 caccgatgac gggcggacat gcgcctcata acggaacata ctgacacact ccgtctccgc
     1201 tcctgccgat gtgtccgggt taaacggaaa ctcccccgcc tgtgacagcc ccgccacatc
     1261 gtcgcacagc gtcagcttct gctccggact gtccgccgca aaccgctcaa agaagaaaag
     1321 accctcctcc gcccatagcc gggacagaaa cgccaggtcc gactccccgt actggacaca
     1381 gaactcccgc gccggatggt cctcataaaa cagcggcgtc cactccgtga cgccgttctc
     1441 gttcagtaac gtggccgata tcgtccggat atcctgctgc tggaagatac gaaagttctg
     1501 ccgcagcccg caccgccata acggcggctc aatacgcagg tgataacgca tctgccagcc
     1561 gttattctcc tgcatgccaa aacccgccac cacgccggtg acataacgct gcgccactgc
     1621 gccctgccag atggtcagtg tcgccttctt ctccagcagc atctccgccg tctgcataaa
     1681 agagtcgctg gcaacatcca cactcatcac aaacggatat gactggttct ggattaacct
     1741 gaaattcacc actgcaaagg tgtccggctc ctggccgtca acctccagcg taaagcgaag
     1801 accttttaag gacataaata cctctcagac atatcagcct atatttaccg taccgccacc
     1861 aataaccacg ccgccgcagt caacagcatc accgcttctg gcggcgggca ggccgtcaat
     1921 aaagacggtt gaagaacctc tggcgatttt acggggatga gggggatgct tcggtttagc
     1981 atgcggcgtc agcggatctc cctgacgggc tgcgggaaga ccatcgataa agacggtgga
     2041 tgacccggcg gtgatgacgg tctcgtaata accgtcgtgc tgggtgcctt tgtcggtgag
     2101 tttggctgct aaaggcataa ttcctcctgt tcagaatttc tacagcagca ggtttcaaaa
     2161 atcgggagaa atattcaacg aaatattaat cacttcaccg gatgttctga tgcgtacctt
     2221 cactggtcta tttaagtaat acaaattgtt ggcgtgtatt aagttccatt ttgttctcct
     2281 taattatgta aggagtattt gatcatgtat aagcaataaa aacagcttca ggaaataagg
     2341 aatatcgcaa tttttaaaca taaaatgcga atcgttcaat acaaaaaagc agcgttgcgt
     2401 aatctttccc tgattaaata cgctaccgcc ctaccctgat gacatgatct gcactgc
//
LOCUS       DAYPHN010000280         2447 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000280 DAYPHN010000000
VERSION     DAYPHN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.284"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..131
                     /locus_tag="ACG0BW_004727"
     CDS             <1..131
                     /locus_tag="ACG0BW_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HFK7261574.1"
                     /translation="LVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            215..694
                     /locus_tag="ACG0BW_004728"
                     /pseudo
     CDS             215..694
                     /locus_tag="ACG0BW_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            869..2097
                     /locus_tag="ACG0BW_004729"
     CDS             join(869..1184,1184..2097)
                     /locus_tag="ACG0BW_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFK7261575.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
BASE COUNT          639 a          560 c          722 g          526 t
ORIGIN      
        1 ggttggtggg gcaaggcaag aagctgttcc gctggcggct acccgaaggt aatccctatc
       61 tgaggacgga gaccagaaac acgtatacat cgcgctttac agaagtggca atggcgttcg
      121 ccagcattta aatggagagc cggatgcgct gaaaggtgta cgtccggttc ggggaggaga
      181 ggcagggaaa tagtccgact acgccctgcc tcttactcta ctcagcacat ggcagggggt
      241 cgtctatgtg gcgttcatca ttgatgtgtt tgccggatac atcgtggggt ggtgggtctc
      301 atcgtctatg gaaacgacat tcgtgctgga tgcgctggag caggcgttgt gggcccgccg
      361 tccgtccggc accatccatc acagcgataa aggctctcag tatgtgtcac tggcctatat
      421 ggagcgatta aaagaagcaa aactgctggc atcaacaggg agtacaggcg actcgtatga
      481 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      541 ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact ggtataacaa
      601 tcgatgattg ctgggaaggc tgggccatac tcctccggca gaagcagaaa aagcttatta
      661 tgcttccatc ggaaacaatg atctggcagc ctgagttcac agataaaaca ctctccagga
      721 aacccggggc ggttcagtaa tatctgatcg taacggacgg acgctggaat taaccgatag
      781 actggccccc tgaatctcca gacaaccaat atcacttatt taagtgatag tcttaatact
      841 agtttttaga ctagtcattg gagaacagat gattgatgtc ttagggccgg agaaacgcag
      901 acggcgtacc acacaggaaa agatcgcaat tgttcagcag agctttgaac cggggatgac
      961 ggtctccctc gttgcccggc aacatggtgt agcagccagc cagttatttc tctggcgtaa
     1021 gcaataccag gaaggaagtc ttactgctgt cgccgccgga gaacaggttg ttcctgcctc
     1081 tgaacttgct gccgccatga agcagattaa agaactccag cgcctgctcg gcaagaaaac
     1141 gatggaaaat gaactcctca aagaagccgt tgaatatgga cgggcaaaaa agtggatagc
     1201 gcacgcgccc ttattgcccg gggatgggga gtaagcttag tcagccgttg tctccgggtg
     1261 tcgcgtgcgc agttgcacgt cattctcaga cgaaccgatg actggatgga tggccgccgc
     1321 agccgtcaca cggatgatac ggatgtgctt cgccgtatac accatgttat cggagagctg
     1381 cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga acttgatggt
     1441 atgcctgcga tcaatgccaa acgtgtttac cggatcatgc gccagaatgc gctgttgctt
     1501 gagcgaaaac ctgctgtacc gccatcgaaa cgggcacata caggcagagt ggcagtgaaa
     1561 gaaagtaatc agcgatggtg ctctgacggg ttcgagttcc gctgtgataa cggagaaaaa
     1621 ctgcgagtca cgttcgcgct ggactgctgt gaccgtgagg cactgcactg ggcggtcact
     1681 acgggcggct tcaacagtga aacagtacag gacgtcatgc tgggagcggt ggaacgccgc
     1741 ttcggcaacg agcttccggc gtctccagta gagtggctga cggataatgg ttcatgctac
     1801 cgggctaatg aaacacggca gtttgcccgg atgttggggc ttgaaccgaa gagcacggcg
     1861 gtgcggagtc cggagagtaa cggcatagca gagagcttcg tgaaaacgat aaagcgtgac
     1921 tacatcagtg tcatgcccaa accagacggg ttaacggcag caaagaacct tgcagaggcg
     1981 ttcgagcatt ataacgaatg gcatccgcat agtgcactgg gttatcgctc gccacgggaa
     2041 tatctacggc agcaagccag taatgggtta agtgataaca ggtgtctgga aatatagggg
     2101 caaatccaac cgatgatcgc agcacagaga gtaggggatg cggacggtca aagatcgtgg
     2161 agttggattt tataaatctg tctccagtag tagatgaact gaatacaaag ttgccagaga
     2221 gtggtctgtc aggcagggaa tcagaaaccg gatattattg actggtacat tgtgtcgggc
     2281 tgtgtacgtt ctccccggac agacagtctc agttcgtaga atctataaat tactgctact
     2341 gatgctgccg gggaaaggtg taacgaaaaa gcagcctccg ttaccggaca tcaagaaggc
     2401 tgaatgaagt ttatggcgtt tgtattttgt aatgtctggc catgcag
//
LOCUS       DAYPHN010000281         2444 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000281 DAYPHN010000000
VERSION     DAYPHN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.285"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            245..475
                     /locus_tag="ACG0BW_004730"
                     /pseudo
     CDS             245..475
                     /locus_tag="ACG0BW_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(1494..1718)
                     /locus_tag="ACG0BW_004731"
     CDS             complement(1494..1718)
                     /locus_tag="ACG0BW_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261576.1"
                     /translation="MDHPILQKTIVLFILALWVIDPFRTKRRKHTESTIEEADARERH
                     KWRYLRWGVRTIQIAATVYMFAMLIQFLLK"
     gene            complement(1960..2064)
                     /locus_tag="ACG0BW_004732"
                     /pseudo
     CDS             complement(1960..2064)
                     /locus_tag="ACG0BW_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300215.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(2061..2284)
                     /locus_tag="ACG0BW_004733"
                     /pseudo
     CDS             complement(2061..2284)
                     /locus_tag="ACG0BW_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          675 a          553 c          520 g          696 t
ORIGIN      
        1 aatatgtatc cttttcaagc tgttcagcgg cgtaagaaaa agtattaatt atgaattcga
       61 cgatttaacc atatctgtct gattggtaat catccgtgat taatgctggt ctattgatta
      121 cgccagcatt aaccgcttag ggatgtattc ttcctgttca tatagccctc gaaaaagaga
      181 gaaaataaaa ctgtgtccaa tattaattta ccactccaga ccgaagcatc ggtgggatta
      241 gcagaatgat ctgcgtctga tgttgaggct ggctcttgcc ggcgcaggca taacaattgc
      301 cactcaggaa actttcaggc catatattga aagcggtaag cttgtatcgc tgcttgatga
      361 ctttcttcca caatttccgg gcttctatct gtatttccca cagcgtcgca atattgcacc
      421 aaagctccgc gccctgattg accacgtcaa ggaatggcgg cagcaattgg cttaaatgtc
      481 tgcacctgca ctgcctgatg tcagaacagt attttgatga attgccaggg ttacaatggc
      541 acaaatacgg cacaggagga aaatgtggtg taattgaatg tgaggtaact acttgattta
      601 aatggtgccg ataagaggag tcgaacctcc gaccttcgca ttacgaatta taagaatccg
      661 cttctaattc aaagcattac cccatcaaca ctgcgctcac acgtcccacc acatcaaaac
      721 atgtaaagcc ttgcaagtca ttgcgaggcc ttatgtgtct cagttttgtc ccaccttgta
      781 ttacgacttg catagccaat gaagataaat gtgacgacaa acggcgcagc agtcttcttt
      841 tccttcatac tttccccacc cagcatgcat accttctacc ataactgtag ttaatgtctg
      901 ttatgagcga aaagcggaca ttcctgtaca aagcatggcg ttggaataca gtaagatcat
      961 tgattaataa gtgagtcgaa tcagaacagt agccaatgaa acaaaaaatt cgcaaacgta
     1021 accaagactg gatatcaagg caattgaaac gtgcgcaaaa gaggaaatgc cgttaagttt
     1081 ctttattaac tttccctcaa tatgagcaac tgcttgtaat ggtgtgcgcc ttaaacggca
     1141 tggtcgtctc aagccggact ggagtcgcgc attgtttcat tagggttggg gagaagtgcc
     1201 tattgttggg taaaaagtag tgtttactgg tttgagggat ttgataagga gcaattgcct
     1261 gttgaatggg ggcgctgtgg gaagatgcct gaatatattg gttttgattt gatctcacag
     1321 tgcaacacac aatcaactct tacagtctgc tgtgagcaaa aattgcacat ttctaacagc
     1381 cttccgtgtg aatcaacggg gagcaggtca ctggcacaga ggggacgttt aaccccgtcc
     1441 ggcctccgct gaattggctg ccaattccgt ccggtcgtac ctgcctgagc cgtctacttc
     1501 agtaaaaact gaatcagcat agcaaacata tatactgtcg ctgcaatttg tattgttcgt
     1561 actccccatc tgagatatcg ccatttatga cgttctctgg catcggcttc ttcaatggtg
     1621 gattcagtat gctttcttct ctttgtcctg aatggatcaa ttacccatag ggcaagaata
     1681 aagagaacga ttgttttctg aagtatggga tgatccacga agagtgactc cggcctgctc
     1741 gctgatacaa tcagaataga tgaacaatcc cgaaataatc aacactaaat gtatggcaat
     1801 tatttatttt cgtcctggct ggactttgtg acctgctccc aacaaattaa catagcacaa
     1861 tgtaagcaag ttctgctttt ggcacatagc agtcctagag acactggcgt aaagccatgg
     1921 aggatcggtg ggaggaggta aaaatcctct catgcaaaaa atacgcaaaa tcgataacag
     1981 ttggaaatca ttcaatactc gcactatcgg aagttcacca gccagtcgta gcacgttctt
     2041 gcatacgacg tgctacggtt tcatttatct ccgaccggaa acttcttata cagagtcgat
     2101 acgccaacat cataaatgat cgccaccttc tggcgaggaa ctcctgatgc aattagtcgc
     2161 ccggcctgcg cccattgttc atctgttagg tttggcctac ggctacctat ccaacctcca
     2221 gtgagtgctc agctaatccc gcctgcgttc gctcaacaat catttccctt tccattttgt
     2281 ccaattgttc ccagtacatc atggagagcc ccattagtgc tctggtatta gtgatttcta
     2341 atatttggag tttagcagat ggcacaaccc tgattgtgtt cccttcaatt gatctattat
     2401 ttttatcagt tatttagtat gcttacctta tcaattaaag tttc
//
LOCUS       DAYPHN010000282         2422 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000282 DAYPHN010000000
VERSION     DAYPHN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.286"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(52..225)
                     /gene="yciZ"
                     /locus_tag="ACG0BW_004734"
     CDS             complement(52..225)
                     /gene="yciZ"
                     /locus_tag="ACG0BW_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFK7261577.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            262..390
                     /gene="ymiD"
                     /locus_tag="ACG0BW_004735"
     CDS             262..390
                     /gene="ymiD"
                     /locus_tag="ACG0BW_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFK7261578.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            complement(372..2357)
                     /gene="pdeR"
                     /locus_tag="ACG0BW_004736"
     CDS             complement(372..2357)
                     /gene="pdeR"
                     /locus_tag="ACG0BW_004736"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFK7261579.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESKKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT          657 a          601 c          556 g          608 t
ORIGIN      
        1 aatactgacg cgccgggcat cctcaaatag gaaaacctga aggcacgatt gttaaatctc
       61 ccacggtgcc ttcgggatgt ctggcgtaga ttcagcaacc tggcgcaaga gttctgcttt
      121 aagtatctcg agattatgga tcgcagagtg gtaatcgcct gccacaagga tatctaacac
      181 aatatcaata cgttcggcaa cgcgctgggc atcaaattca gacatcaagg acatccttat
      241 agcaaaaggg gaaaatgaat aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca
      301 acggctaatt atcatccggg aatacgatat ataatgacgg aatatagcgc taagtatata
      361 tattcatcta cttatgcgcg cttcagatag cgtttatacc agcgttcaaa ggcgacggcg
      421 ggcatcggtt tggcaaacaa aaatccttgc cgctcattga tcccgttctt ggttaaaaaa
      481 gcatcttcct ttttactctc cactccttca gcgatcacct gaagattcaa tgcctgggcc
      541 acagcgacga tcgcccggac cagcgactgc gatacaggct gtttgtgaat atcccgaaca
      601 aaaacctggt caagtttaat cgcatcgatc ggaaagcgcg ccagttgcga aagtgaagag
      661 tagccggtac caaaatcgtc cagatgcact tgcgcaccta gttggctaaa ttgttgaata
      721 acagaaagtg ccagttcatc attctcaatc aggcagctct ccgtcagttc aacatcaata
      781 gggcagtatt caaaattgag ttcctggaga acctgtttca gggcggtgaa aatggtttga
      841 tcggcgagct gacgtgcaga aatgtttacc gccactcgca ggttaatacc tttatcccgc
      901 cactttgcca cctggcgtac gacatcgaga atcacccaac ggcctaaagg cacaattagc
      961 cctgactctt cggcgtagga aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac
     1021 tgccaacgta ctagtgcttc cagactgcgc acttcgccac gccaggtgat tttcggttga
     1081 tagtgaataa ccaactgatc gttttccagt gctttacgca agttggtatc cagccagaga
     1141 tattcaaata cccgctgatt catttctggg gtaaaaacgc aaaattgtcc tcgtccgcct
     1201 tccttcgctg tgtacattgc tgtgtcggcg tgacgaataa tagccgcgct gtccgaacca
     1261 tgttcgggag agagtgaaat acccactgaa cagctggtat aaacttcaat taaaccaatg
     1321 cggaagggga gtcgtaagcg ggtcaaaatt cgtgatgcca ttgcttccag cgcgctttgt
     1381 gaggtattgg atgccagtac cagaaactca tcgccacctg gacgcgccaa cacctggtca
     1441 tgttcgagac agcttaaaat ggccaatgac acgtcgcgta atagctggtc accaaacaaa
     1501 tgcccatagg cgtcgttgac ctttttaaaa ttatccaaat caagatacac aaccccaact
     1561 ttattgttat ctgcctgatt aatagcgtga tcgattaact cctgcattgc gttacgattc
     1621 ggcagtccgg tgatactgtc ggtatttgcc agaatgcgca gtcgctcctg agcgcggcgc
     1681 tcttcggtaa tgtcggtgcc ggaacagatt aaaaaaatct cgtttttgcc actaccgctg
     1741 tggacaaatt tattgcgaaa cagaaacagc cgctggcctt tacgtgttgg tatccacagt
     1801 tcgacttcat atgcattgcc gcttcgaaaa aatacacggt tattgcgcct ggatgccgca
     1861 gcttcacgac ggctcataaa cagtttaaac acgctttgcc caatgacgtc gtgttctttc
     1921 aaccctgtgt aatcttcaca taaccgattg aagcgctgga tattcccccg actgtcgaga
     1981 atcactattg cggaatgtgc ttcagaaact acctgctcag caaaggaaag cccgtgtgat
     2041 aaatcacgag caaccgccgg tgtgtcatgc catgccgacg cgttgccagc ccattcccgt
     2101 ttattgattt ttcgtcctac cagatgcaca gaaagatcgc tttcatcgac ggtcagactc
     2161 atcatcagac tggaagtgat gactgtcatt tccctgatgc gagcagcctg ttcggcagat
     2221 aactgcaacg tacgttcagg ttctgtggtt tcggtggtag aaaagcgcaa aacatcgctg
     2281 ctttccgtta accgccagta tggattgtgc gatccgagaa agttgtacaa cgttgtggac
     2341 tccctaacgg ttttcatgat gcatatcctc cggttaacag cggagtccgc ggttaaataa
     2401 aaggaacaac atgacttaca tg
//
LOCUS       DAYPHN010000283         2403 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000283 DAYPHN010000000
VERSION     DAYPHN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.287"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..302
                     /gene="pspC"
                     /locus_tag="ACG0BW_004737"
     CDS             <1..302
                     /gene="pspC"
                     /locus_tag="ACG0BW_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFK7261580.1"
                     /translation="GVCAGIANYFDVPVKLVRILVVLSIFFGLALFTLVAYIILSFAL
                     DPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREMERYVTSDTFMLRSRFRQL"
     gene            311..532
                     /gene="pspD"
                     /locus_tag="ACG0BW_004738"
     CDS             311..532
                     /gene="pspD"
                     /locus_tag="ACG0BW_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFK7261581.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            607..921
                     /gene="pspE"
                     /locus_tag="ACG0BW_004739"
     CDS             607..921
                     /gene="pspE"
                     /locus_tag="ACG0BW_004739"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFK7261582.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIASAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            1040..>2403
                     /locus_tag="ACG0BW_004740"
     CDS             1040..>2403
                     /locus_tag="ACG0BW_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFK7261583.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAA"
BASE COUNT          556 a          600 c          664 g          583 t
ORIGIN      
        1 gcggcgtttg cgccgggatt gccaactatt ttgatgtacc ggtaaaactg gtgcgtatcc
       61 tggtggtgct gtcgattttc ttcggtctgg cgctgtttac cctggttgct tacatcattt
      121 tgtcatttgc gcttgatcca atgccggaca acatggcctt tggtgagcag ctaccttcca
      181 gcagtgaatt gctggatgaa gtcgaccgtg aactggcggc aagtgaaacg cgtttacgcg
      241 agatggaacg ttatgtcact tccgatactt tcatgttacg tagccgtttc cgtcaactgt
      301 gaggaaagtt atgaatactc gctggcaaca ggccgggcaa aaggtgaagc ctggtttcaa
      361 attagcaggc aagctggtac ttcttaccgc actgcgctat ggcccggcgg gtgtagctgg
      421 ttgggcgata aaatcagttg ctcgccgacc actgaaaatg ttgctggccg tggcgctgga
      481 accgctgtta agtcgggctg ccaataaact ggcgcagcgt tataaaaggt gaggggagtt
      541 tcgcaaaaat tgttaaagct aaggcatatt atggatggca attttcatcc atagaaggac
      601 gcttacatgt ttaaaaaagg cttacttgct ctgacactgg tgttttcact gcccgttttc
      661 gccgctgaac actggatcga tgttcgtgtt ccagagcagt atcagcaaga gcacgttcag
      721 ggggccatca atattcccct gaaggaagta aaagagcgca ttgcctccgc cgttccggat
      781 aaaaacgaca ccgtgaaagt gtattgcaat gccggacgcc agtcagggca ggcaaaagag
      841 atccttagcg agatgggata tacccacgtt gagaacgccg gtggcctgaa agacatcgca
      901 atgccgaagg tcaaaggtta acaaattact cattgagcgg tgtaaacaac gccgcttatt
      961 catttgataa atcaaaatta acccacatta ttatctccag tatctaatcc tttctacagc
     1021 tttctggcac aggacagcaa tgaagcgact taaaaatgaa cttaatgcgc tggtgaatcg
     1081 gggtgtcgac agacatctgc gcctcgccgt aaccggacta agccgcagcg gcaaaacagc
     1141 gtttatcact gcgatggtca atcagttgct caatattcat gccggagcac gcttgccgct
     1201 gttaagcgcg gtgcgtgaag agcgcctgct gggcgtaaaa cgcattcctc agcgtgactt
     1261 tggcattccg cgcttcacat atgatgaagg actggcgcag ttatatggcg atcctcccgc
     1321 ctggccgacg ccaacgcgcg gcgtcagtga aatccgcctg gcgctacgtt ttaaatcgaa
     1381 cgattcgctg ctacgccact tcaaggacac ctccacgctg tatctggaga ttgtggatta
     1441 tcccggcgaa tggttgctcg acctgccgat gctggcgcag gactatttaa gctggtcacg
     1501 ccagatgacg ggcttactca atggtcagcg cggcgaatgg tcggtcaaat ggcgaatgat
     1561 gtgcgaaggg ctggacccgc tagcacctgc cgacgaaaac cggctggcag acattgccgc
     1621 cgcgtggacc gattatctcc accactgtaa acagcagggg ctgcacttta ttcagccagg
     1681 gcgctttgtc ttgccaggag atatggcagg tgcgcccgcg ctgcaattct tcccgtggcc
     1741 ggatgtcgat acctggggcg agtccaaact ggcgcaggcc gataagcaca ccaatgccgg
     1801 aatgctgcgc gagcggttta attattactg cgagaaggtg gtgaaggggt tctataagaa
     1861 tcattttctg cgctttgacc gccagattgt gctggttgat tgcctgcaac ctctcaacag
     1921 tgggccacag gcatttaatg atatgcgtct ggcactgacg cagctgatgc aaagtttcca
     1981 ctacgggcag cgtaccttgt tccggcgttt gttttcgccg gttatcgata agctattgtt
     2041 tgctgccact aaagcggacc atgtgaccat cgatcagcac gccaatatgg tttcattact
     2101 gcaacaactg attcaggatg cctggcaaaa tgcggcgttt gaagggatta gtatggattg
     2161 cctggggctg gcctcggtgc aggcgaccac cagcggcatt attgacgtca acggcgagaa
     2221 aattccggca ttgcgcggta accggctcag cgatggtgcg ccactcaccg tttatcccgg
     2281 tgaagttcct gcgcgtttgc ccggtcaggc gttctgggac aaacaagggt tccagtttga
     2341 agcgtttcgc ccgcaggtga tggatgtcga caaaccgctg ccgcatattc gtcttgatgc
     2401 cgc
//
LOCUS       DAYPHN010000284         2367 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000284 DAYPHN010000000
VERSION     DAYPHN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.288"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            130..762
                     /locus_tag="ACG0BW_004741"
     CDS             130..762
                     /locus_tag="ACG0BW_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFK7261584.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            762..2018
                     /locus_tag="ACG0BW_004742"
     CDS             762..2018
                     /locus_tag="ACG0BW_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFK7261585.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQITVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMTASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            2045..>2367
                     /locus_tag="ACG0BW_004743"
     CDS             2045..>2367
                     /locus_tag="ACG0BW_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261586.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNAS"
BASE COUNT          667 a          496 c          641 g          563 t
ORIGIN      
        1 atacggaagc aggcttagta agacatgcca gacctaatta aacaccccag aaaacaagtt
       61 ggttagcaga tgcagtaaac aagttgtttt agagcgcatg gacgcgctct gtgtgtttta
      121 gagggatcta tgacggtcga aaaagacgag gtaaaaactc gcctgacacc agcggagtgg
      181 gccgaagctg aagccaaatg gacgtcaggc gaatatacac tctcaaagct ggaggaagag
      241 tacggcattc gtcgtgaaac actctccaga catttcaaaa agcgaggatt agagaaaggc
      301 gcggactctg ttgggaagat ggttcgtgag tctcttaaat ctgacgcaga gcttcgcgct
      361 aaagcccgtg cggaaaagat agaagaacgt cgtacacgtt atgacggctg ggcgtatgcg
      421 ttggggcaga tggtgatggt cgaagtcact acggccaaac gtgagggtaa gcctttaggg
      481 gcgattgagg attctctcaa gagcttacag agagccagta atacccttgc aaaatgcttt
      541 gaagtttcgt ccaaagcatt gggcatggat catgcggaaa atgacgagga agaaattccg
      601 aacctggtat ttggtgagct tacgccttcc caggtggcga aattacgtca ggaagacgac
      661 gagcctgaaa tcatcgatga cgaattgctt gagacgttgg aagaagaagc tctaagcgaa
      721 tttgatgcga cagatgatgg aagtgaaggg gaggacgaat aatggcaatc ccgtcctcgc
      781 tcagtcttgt gcaactgcat tctggacaga tgaaagtctt ccagtctccg catcgattta
      841 aagttgtttg tgctggtcga cgctggggaa aatcccggtt gtcgatctcc actattattc
      901 gtgcggcggc aaaggaaaaa aagcaaaggg tctggtatgt cgctcctact taccagatgg
      961 ctcgccagat tttgtgggac gatctacagg aagttctgcc tcgtaagtgg gttaggaaaa
     1021 agaacgacac cacgatgaca atcgtgttga agaacggttc ggagatcgcc ctcaaaggtg
     1081 ctgataagcc tgacactctg cgcggcgtag cgttgcattt tgtagtgctt gatgaatttc
     1141 aggatatgaa ggctgacacc tggtacaagg tgttacgacc tactctttca tcgacacgcg
     1201 gcggtgcact gatcattggt acgccaaaag gcttctcgga atttcacaaa ctgtggacta
     1261 taggccagaa cgtagagctg caaagaaagg gacagtggaa gagctggcag tttgtaactg
     1321 ccgattctcc gtttgtgcct acggcggaaa ttgaagctgc taagaacgat atggacccga
     1381 aatcgttcgc tcaggagtac ctggcgagct ttgagaacat gtccgggcgc gtttactacc
     1441 cgttcgatcg taacgtgcat gtaaaaccgc ttcagttcaa ccctcggttg cctatatggg
     1501 tagggcagga cttcaacatt gacccgatgt cttcagtaat tttgcaacct cagccaaatg
     1561 gtgagctatg ggcaattgat gaattggtgc tcttttcctc taacacggca gaggtttgtg
     1621 atgagcttga gagacgcttc tggcgctgga aatcacagat aacggtattt ccagatccgg
     1681 caggtgctta tcgccaacat gctcgcgggg agtctgacgt cgacatattc aaagagaagg
     1741 gattcttacg tgtcgattat tcgaaaaagc acccgccaat tgcggatcgt gttaatgctg
     1801 ttaaccgaat gctgatgacc gcatctggag atatccggtt gtatatcgat ccgaagtgca
     1861 agcatttgat tgattcactg gaaaaagtca tctacaagcc tggaacacga gatatggata
     1921 agacaggtgg cattgagcat agtgcagacg cattgggcta tccagtacat cgtaggtatc
     1981 cagtcaaaaa ccgtgttatt cttggtggtt ctcgataggt aagtaattat ctaaggttat
     2041 tcaaatggaa ttgaacgaca aacaaattaa ggatctggtg gcgcgacgcc acccggaata
     2101 cgaaaagaaa aaagaacatt gggacttcct cgccagcact tacgctggcg ggcgtggttg
     2161 gtttacagac aatatctttc gttactttaa agagggagat caggagttta aggagcgagt
     2221 tgagcgtgct tatcgcttca accacactcg tgaggtggta aacctcatca acaaatatct
     2281 ctttaaagaa gacattcata gaaatatcga agaggcacca gagcagatcc gcaatttctg
     2341 gaaacgtgcg actcgccaga atgcctc
//
LOCUS       DAYPHN010000285         2344 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000285 DAYPHN010000000
VERSION     DAYPHN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.289"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            95..325
                     /locus_tag="ACG0BW_004744"
     CDS             95..325
                     /locus_tag="ACG0BW_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261587.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSQ"
     gene            908..1498
                     /gene="rnhA"
                     /locus_tag="ACG0BW_004745"
     CDS             908..1498
                     /gene="rnhA"
                     /locus_tag="ACG0BW_004745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFK7261588.1"
                     /translation="MTTKTPARSQAKTRKKAKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNK"
     gene            1647..2141
                     /locus_tag="ACG0BW_004746"
     CDS             1647..2141
                     /locus_tag="ACG0BW_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261589.1"
                     /translation="MARQTYFTSATKRPRSLRQILAELFSGRVMSRLDELETTVRLLN
                     ERLDNQASVVANVGAIVASGSSREAKSTRPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRPEPFDAGFIHHHTFVDDNYHHSSGASCHSGWDGGGCDTSSSSSYSG
                     SCCD"
     gene            complement(2163..>2344)
                     /locus_tag="ACG0BW_004747"
     CDS             complement(2163..>2344)
                     /locus_tag="ACG0BW_004747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261590.1"
                     /translation="PSEITKYRTNWKNKNPNYTSQGHASLKLEIARANKFYNVRYPTG
                     DHKGKNCQPNFSKVI"
BASE COUNT          635 a          498 c          582 g          629 t
ORIGIN      
        1 gagactccag acctgaacat cactacagaa tccactagaa ccccttccca gacgctttaa
       61 aatcgatttt atgaaccact ttaaggaaac caacatgtca tacccgacta atgtcgttgc
      121 gctcgtagag agcgattttc tggcccaggc tcgtgaaatg atgaaagatc gtgagcaggc
      181 tttcaacttg tacgaatggg caattaagtg cttgcatctt ggcgagcatc gcgaacttgt
      241 tgaacagctt ttaggtgagt tgatcaatga ggtgtttgcc ttgaatgttc aactacatgg
      301 tagagaaaat aatcaatcac aatgatagat aagtacaaac tattcataaa gtgaattgta
      361 agtgctaaga tctgatagtt tccagtcgta gactggagac tcgacctgat gggtgggggt
      421 aagcgtcact ggcgtcaggt ttaaaaaagc tcactaccag cgtagaactg gtgccgttta
      481 ggtgtcgggg aagggggaac caaagtgagc agagacaagg gtcactttat gattgtcgag
      541 tctggggtgt ttcgagaggt tgaatccagt actccccttc ataaagtgtg ggaagatctc
      601 ggttctgggg tgctgtcatc cataacttcc caagtctaag ctggcagtag acttagacca
      661 taacttttca ggttatgaaa cgaccaggtt ggtgagagat ttttatactc acctccctgg
      721 gagagtatta cctgaaaaga caacctctca cttcgttcga ggtgaacttc actcacttcg
      781 ttcgttcagt tcaggtttat aaaaacctgt tctgggaagt aatttgttta ttttaataat
      841 tattaacacg cacgcgtgtg cgcacgcgcg aggaaaaatc ggcgcggcgc ttgattcagg
      901 agtttatatg acgacgaaga caccagcccg atcgcaagca aaaactcgca aaaaagccaa
      961 aaacaaaaat tccccccgca ccaatcccac aacgcctgtc gtagagttca atccccagct
     1021 taaaaccgtg aaaatcttta gtgatggctc ttgccttaaa aatccgggtg gcccgggcgg
     1081 ttacggtatc gttctccagt atcgtggtga ggaacgcgag ttctcagatg gttttcatag
     1141 caccaccaat aaccgcatgg agatgatggg ggcacttatc gggctggagc gtttgaaata
     1201 tccatgcaac gttattttgc actctgatag ccagtatctg aaaaacggca tgacacagtg
     1261 gatgaaatgg tggaaacgca atggatggat gacttctgac aaaaaaccgg taaagaatgt
     1321 tgatctgtgg aagcgtctgg atgaggccgc aagtcgacat aatgttcgct ggaagtgggt
     1381 taaaggccac gccgggcatc gtgagaatga aatatgcgat cgactcgcga agatcgcagc
     1441 tttttcagca gcagatatgc ctcacaagaa agatattggt tttgtttata ataagtagta
     1501 agtaagtgtt tacctatcat tttaaatcat gtatcttatc ggcgtcagga tgacaatgtg
     1561 tcggtaagac acagttccag gatggaacga gaaaggcggc tggcaatcgc cagccgcaac
     1621 tctttctgac actggatggg gtccacatgg cacgtcaaac ctatttcact tctgcaacta
     1681 aacgtcctcg ttctttacgt caaattttgg ccgaattatt tagcggtcgt gttatgtcac
     1741 gtcttgatga actagagact accgttcggt tgctgaatga acgtttagat aatcaagcgt
     1801 cagttgttgc gaacgtgggg gcgattgttg cctctggttc ttcacgcgaa gcgaaaagta
     1861 cacggccttt agtgaaggag aaaaacaaca aggacagttc gaatggaaaa ttttcaaaga
     1921 aagaggctga aaccaatggc ctacgttctc attatagttt cactggcgac ggtagccgtt
     1981 ccagccggcc agagcctttt gatgccggat tcatccatca ccacaccttc gtcgacgaca
     2041 attaccacca ctccagtgga gcgtcctgtc actctggatg ggatggcggt ggatgcgata
     2101 cctcaagttc atccagttac tcaggatcat gctgtgacta aggcggttgt atgaactggt
     2161 tttcaaatca ctttggaaaa atttggctgg caattcttgc ccttatggtc gccggttggg
     2221 tatcgaacat tataaaactt gtttgctctg gcgatctcca gtttcaggct ggcatgacct
     2281 tggctcgtgt agttgggatt tttgtttttc cagttggttc ggtacttggt tatttctgac
     2341 ggct
//
LOCUS       DAYPHN010000286         2315 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000286 DAYPHN010000000
VERSION     DAYPHN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.290"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..551
                     /locus_tag="ACG0BW_004748"
     CDS             <1..551
                     /locus_tag="ACG0BW_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7261591.1"
                     /translation="QISHIWGTRIRLELEGKTRDLALFNMALDSKLRGCDLVKLKVSD
                     VAYGRLVSSRATVLQQKTGSPVQFEITKGTREAIAALIKLDYLHNKDFLFRSRIGTNQ
                     HISTLQYNRIFHGWAGKLGLEDSLYSTRSMRRTKPYFIYKKTKNLRVIQLLLGHKKLE
                     STVRYLGIEVDDALEIFESIEV"
     gene            905..2002
                     /locus_tag="ACG0BW_004749"
     CDS             905..2002
                     /locus_tag="ACG0BW_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFK7261592.1"
                     /translation="MAVENRERLNEILRELKDGMSSNPVSVREFISWFGAERRGYMVA
                     YKIRKALESLGVKTEPDFDSVPLDAKITIYAPPGNDATPHEAGECLHEETESSNAELA
                     TPETVFQPHDELISGAVSEPAFRVSRLEASDVKLVTVKPDATLTEAITLMLRHDYSQL
                     PVMTSERDVKGVISWESIAPILALTQSESAVVRDYMKPHREINASDSIFSALPRIIEY
                     AYVLVRSSDQRISGIITTTDLSSQFRQLSEPFLLLSEIENHIRKLIDGKFTKDELVSI
                     VNPFDSERVIESVADLTFGEYIRLFENPELWKKTELQIDKKIFTKELDKVRIIRNDVM
                     HFDPDGISEENHELLHNFVRFIHTIQSLSRK"
BASE COUNT          716 a          458 c          519 g          622 t
ORIGIN      
        1 ttcagatatc tcatatctgg gggacccgaa tccggcttga actggaaggc aaaacgcgcg
       61 atttagctct gttcaatatg gccctggaca gtaagcttcg aggctgtgat ctggtcaaac
      121 tcaaagtatc tgatgtggca tatggtcgct tggtttcaag cagagcaacg gtgttgcaac
      181 agaaaaccgg tagccctgtg caatttgaga taaccaaagg aacaagagaa gctattgctg
      241 cattgataaa gcttgattat ttgcacaata aagacttctt gttccggtct cgtatcggaa
      301 ctaatcagca catatcaacc ctacaataca accgaatctt tcatgggtgg gcaggaaagc
      361 ttggtctcga agattcgctt tacagcacac gttccatgag aagaacaaaa ccctacttta
      421 tctacaagaa aactaagaat cttcgggtga tccaacttct gttgggccat aagaaactgg
      481 aaagcacagt ccgttatctg ggtattgaag tagatgatgc attagagatc tttgaatcga
      541 ttgaagtcta aggttttcag ggctgcaaca gcagccctgt gccagaaacg gacggtgggc
      601 ctcgagtgga aaaataacga ttaatattgc agcttgttgt ccgagtcttg tatttttatc
      661 aaattctaaa tgaataaggc atctactaaa ctcaacgcgc tggcactagc ggtaagggca
      721 attcgtattc tggcctcctt atactgaaca ttaggaatct gagctatgtg tatgggatgg
      781 tgcaatctga ggtgagctga aaaagtcagt gcagcctcgt tgtccagatt ttaaaaaaag
      841 cgtgagaagc ctgaatcaag attgaaacct aacagtagcc gacaacctac atggagaaaa
      901 ttttatggca gtagaaaatc gagaaagact taacgaaatc cttcgtgaac taaaagacgg
      961 tatgtcctca aacccagtat cagtgcgaga attcataagc tggtttggcg cagagcgtcg
     1021 aggctacatg gttgcgtaca aaattcgcaa agcattagaa agcttaggag ttaaaaccga
     1081 acctgatttt gatagtgtgc cgctggatgc aaaaattact atctatgcac ctccggggaa
     1141 tgacgcaacg ccccatgaag caggtgaatg tttacatgaa gaaaccgaat caagtaacgc
     1201 cgaattggct acacctgaaa cagtatttca gccgcatgat gaactaattt ccggtgcagt
     1261 ttccgagcca gccttccgtg ttagtcgact cgaagcatcg gatgttaaac tagtgaccgt
     1321 gaaacctgac gctacattaa cagaagccat cacattgatg ttgcggcatg actactctca
     1381 actacccgtt atgaccagcg agcgagatgt taaaggtgtt attagttggg aaagcattgc
     1441 tccaatacta gcgttgaccc aatcagaatc tgcggttgtg cgtgattata tgaagccaca
     1501 cagggaaata aacgccagtg actctatttt ttctgcacta cctagaatta ttgagtatgc
     1561 ttacgtatta gtgcgatctt ctgatcaaag aattagtggg attataacta ctaccgatct
     1621 tagttctcag ttcagacagt tgtcagaacc ttttctttta ctatctgaaa tcgaaaatca
     1681 tattcgtaag ctgatagatg gaaaatttac gaaagatgag cttgttagca tcgttaatcc
     1741 atttgacagc gaacgggtta tagagtcggt cgcagattta acctttgggg aatatatacg
     1801 actttttgaa aatccagaat tgtggaaaaa aaccgaactt caaatagata agaaaatatt
     1861 tactaaagag ctcgacaagg tcagaataat tcgtaacgac gtgatgcact ttgacccaga
     1921 tggtatatca gaggaaaatc acgagcttct tcataacttt gtaaggttta ttcatactat
     1981 acaaagccta tcaagaaaat aagagtgacc tgctgcctgt tgattaacac gcagcaatgt
     2041 tagtaatgcg actatttggg tgtgagatca actgtgaact tccgttcctc gctcataacg
     2101 gaccttctgc tcagttagtt cggtcgcttc gtggtcaaag tggactaaat ggctattcgg
     2161 tgacctgttt taatcaacca agtcttttag agggaaacag gaatctcgtc agtcgaacta
     2221 acggttacgg aagcaggccg gacaaccact ctaaatcata ggtagttgag tttaaaggaa
     2281 gtgccatctt tagactttat ctatgtaaag taaat
//
LOCUS       DAYPHN010000287         2255 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000287 DAYPHN010000000
VERSION     DAYPHN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.291"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(639..1208)
                     /locus_tag="ACG0BW_004750"
     CDS             complement(639..1208)
                     /locus_tag="ACG0BW_004750"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFK7261593.1"
                     /translation="MSRFIPVELHHASRLLNHGPTILITTFDEKSQRRNVMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNKLHHLGAGTFVTSGKRVTAG"
     gene            1383..2228
                     /gene="nhoA"
                     /locus_tag="ACG0BW_004751"
     CDS             1383..2228
                     /gene="nhoA"
                     /locus_tag="ACG0BW_004751"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFK7261594.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQITPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELAMVMAAFDTH
                     PEAGK"
BASE COUNT          589 a          503 c          531 g          632 t
ORIGIN      
        1 gtaaaatcat caagagtaca tatagatcat gagaaagata tttttgatgg ccttaatcaa
       61 atcttggctt tttccgataa taataaacat gagtattttt tatatataaa ggcatattgg
      121 ctcagtttat acttttgtga aatttcacag aacgtgagag aaagagataa gttgtcattt
      181 tattataatg aattttctgt ccgatttcct aatgttgtcc tatatccgtc atgctacaaa
      241 ttgtaaggct tggcacctga aattcattgt taattcgaga cgcttgattg tccactatat
      301 caaaacagtc atacctcgga attttctcgc attagtccat acggtattca atattcgcaa
      361 ctgtgccctg aaataaaaaa ctgcaaaact gctttttctt gacctgtccc aatcacgcaa
      421 gactactttt agtagagacc tccagctatg tcgattttag tgccgcccgc gcattttata
      481 caaaacaggg ctttattaat gaagctataa tacgcaatta ctatgatgtt ggtgaggata
      541 aattaatctt cactaaaaat atgcaatcgt gagataaaat atgcttttaa ctcatattga
      601 aacattgctt aagcaacttg gttgctgcat atgattcttt agcccgcggt gactcgcttg
      661 ccgctggtaa caaacgtgcc agcacctaag tgatgtagtt tattgagttt atcatcatca
      721 aactgccagc ggccttcgac aaatacccgt gcgtctgccg ctgctgatac cacctcgcca
      781 aacagcgtgt cgtatttttc ttgcgcggaa gtcgcaggta gcaagcggca ctccatccac
      841 gccagacatt tttcctcgac cagaggcaaa ccaagtaccg gacctttcac tactggaatg
      901 ccgtagcaat taaatttatc ttcttcacga cccgacacac ttcccaccgc ccacgtccag
      961 ttagttgctg caacgcccgg gataacgatg ccaaatttac cgttacgttc aatcaactcc
     1021 ctggtccatg tcgatttatc taccacgatc gcaacacgtg gcggttcaaa ctctaccggc
     1081 atcgaccagg ccgcagccat cacattacgt cgctgtgatt tttcatcaaa agttgttatc
     1141 aaaatagttg ggccatgatt taacagacgg ctggcatggt gtaattcgac agggatgaat
     1201 cggctcacgg tatcgcctcg ctaagaaggg ggttttaaaa gcgtagctcg taacgctata
     1261 agtaacgaaa ttaacgggat tggcgatttg cgaacgtgat gcatgtccgc gatcgcacaa
     1321 aatagccggt gcggcgtcta ttccaggtta taagttgaga aaaccactaa gggaaacgcc
     1381 tgatgacgcc cattctgaat cattattttg cccgtattaa ctggtcggga gctgctgcgg
     1441 tcaatattga tacgctgcgt gcattgcacc tgaaacacaa ttgcaccatt ccattcgaaa
     1501 acctcgacgt tttgctgcca agggaaatac agcttgatga tcaatcgctg gaagagaaac
     1561 tggtgacagc ccgtcgtggt ggttactgtt ttgagcagaa tggcgtgttt gagcgggtgt
     1621 tacgcgagct ggggtttaac gttcgcagct tgttagggcg cgtagtgtta tcaaatccgc
     1681 cagcattacc gccgcgcacc catcgtttgt tgttggtgga actggaaggg gaaaaatgga
     1741 ttgctgatgt cggtttcggt ggacagacgc taaccgcgcc gattcgttta gttcccgatc
     1801 tcgtgcagat cacgccacac ggagagtatc ggttgttaca ggagggtgat gattgggtgt
     1861 tgcagtttaa tcatcatcag cactggcagt cgatgtaccg ttttgatctc tgcgagcagc
     1921 aacaaagcga ttatgtaatg ggcaatttct ggtctgcgca ctggccgcag tcgcattttc
     1981 gccatcattt gctgatgtgc cgccatttgc cggacggcgg caagctgaca ctgaccaatt
     2041 ttcattttac ccattatgaa aatgggcacg cggtggagca gcgaaatcta ccagatgtgg
     2101 cgtcgttata tgccgtgatg caggaacagt ttggtctggg cgtagatgat gcgaaacatg
     2161 gctttactgt ggatgagtta gcgatggtga tggcggcgtt tgatacgcac ccggaggcgg
     2221 gaaaataatt tatgtcaggt tgccggatgc ggcgt
//
LOCUS       DAYPHN010000288         2251 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000288 DAYPHN010000000
VERSION     DAYPHN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.292"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(1..674)
                     /locus_tag="ACG0BW_004752"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="ACG0BW_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(914..1192)
                     /locus_tag="ACG0BW_004753"
     CDS             complement(914..1192)
                     /locus_tag="ACG0BW_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261595.1"
                     /translation="MKARVLAVLRSLEHKVADYPHLMLTSCTGEVGAASGPLMLAYLA
                     SVMSRTDGPGKKGLLHLSADNGQRAAAIVRERAWCAVGTKMQNGTFCM"
     gene            complement(1318..>2251)
                     /locus_tag="ACG0BW_004754"
     CDS             complement(1318..>2251)
                     /locus_tag="ACG0BW_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261596.1"
                     /translation="YIYITTREKRDDIAPVMEEHLARKNFVSFFFDEPLGEQWGYSGY
                     EGMDKIFSATTSIPFSQYVGVTMENDVFAIGSGFEGLEEPIPYEFAISNTRTIGGRLY
                     VVGSARSVVRRIDRNNWEKLNKGIHLPAQDEIKTIREQMLFLDAAGFSCVDGFSENDI
                     YAAGGKGDVWNFDGDEWRQIHFPSNMLIESICCGQDGYVYIGAQSGTVFKGRKDQWKM
                     IHRGELTLPFRQMVWYENCVWATSDYGVWQIIGDDVVQPDLPSEVKMCTGYISCTEKT
                     LVLAGYYGVARLENGHWDILINCVQAEKGDFCSE"
BASE COUNT          642 a          540 c          388 g          681 t
ORIGIN      
        1 attgcgtaac tgttatgctc tggattattt aattctttca gtgggaattt ttcctcgaag
       61 gctttattta gccgcccctg gtttcttttt acagcgaata aataatcacc gccctgtttt
      121 tgtatcttct ctgcaatatc tttctggcaa cccatcgtat cagttgtgat gatttttcct
      181 ttaatatcca gcatgttaag aagttatggg atagctgtaa tctcattaga tttctcatcc
      241 gtcttgatct gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc
      301 gctcccctgc ggcgactctt gtcataatag tgccggagcg tttttccatc aattgcgatg
      361 acgtctttat catctgaagt atggcaatct cgcatccagt taataaagca ctcgtgaaat
      421 tttgcaggac tgatacagga tacaactctg gcaatggtat cgtggacagg cataccattt
      481 tcaaaatcac catattgctt caaaaaatcg atatgtgttt caccaaaatc ctctatatct
      541 tcccaacctt ctgcacccga aataacggca caaatagtca acagtagaat atccgataat
      601 ttatgttcca ctttccaggc ttgtctgtaa tcgggggtaa tagaaatatg ttccatcaat
      661 tttttaagtt ccattttttt ctccttaatt atgtaaggag tatttgatca tgtataagca
      721 ataaaaaaca gcttcaggta atgaggaata tctcaatttt taaacataaa atgcgaatta
      781 tttagtacaa aaaagcagcg ttacgtgatc tttccctgac agcaaactga atacagaaac
      841 atattggttg gaaatgctat ttgacagcag atcccggtgg gcctgccacc atctcttcac
      901 aagcttatgc ttattacatg caaaacgtcc cgttttgcat ctttgtgcct acggcacacc
      961 atgctctttc cctgacaatt gccgcagcac gctgaccatt atcggcactc agatgtagca
     1021 gtcctttttt tcccggacca tcggtgcggg acatcacaga cgccagatat gccagcatca
     1081 gtgggccgga ggcagcccca acctcaccgg tacaggaagt gagcatcaga tgagggtaat
     1141 ccgccacttt atgttccaga gaacgcagga cagccaggac gcgggctttc atgcttttgc
     1201 cctgaaaggc atggtcattc cgcattgcat ttttaaagtt ttttatccat tatttattct
     1261 gattaataat caaaattgaa atcctgtctt tgaaatgtaa tggcagtgca acaatcacta
     1321 ttcagaacag aaatcacctt tctctgcctg aacacaatta atcaatatat cccagtgacc
     1381 gttctctaag cgagctacac cataataccc agccagaacc agggtttttt ctgtacaact
     1441 gatataacca gtgcacattt ttacctcgct gggtaaatct ggctgaacaa catcatcccc
     1501 gattatttgc cacacaccat aatcagacgt tgcccagaca caattttcat accataccat
     1561 ttgcctgaac ggtaaggtta actccccccg atgaatcatc ttccattgat cttttcgacc
     1621 tttaaataca gtaccgcttt gcgcaccgat ataaacatag ccatcctgac cacaacagat
     1681 agattcaatg agcatgttgc tgggaaaatg aatttgcctc cattcatcac catcgaaatt
     1741 ccagacatct cctttaccgc ctgcggcata gatatcattt tcagagaagc catccacaca
     1801 actaaaacca gcagcatcca ggaatagcat ctgttcacgg attgttttaa tttcatcttg
     1861 cgcagggaga tggatacctt tatttagttt ctcccaatta tttctatcta tcctgcgaac
     1921 aacactacgt gcggatccta caacatacaa tcgaccacca atagtccggg tattgctaat
     1981 ggcaaattca tatgggatcg gttcttcaag cccttcaaaa ccactaccta tagcaaaaac
     2041 atcattttcc attgtaacgc ccacgtactg actgaatgga atactggttg tagccgaaaa
     2101 tatcttgtcc attccttcat aaccgctgta tccccattgc tctcctaatg gttcatcaaa
     2161 aaaaaaggaa acaaaatttt ttcttgccag atgttcttcc attacagggg ctatatcatc
     2221 tcgcttttct cgtgtagtta tataaatata a
//
LOCUS       DAYPHN010000289         2246 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000289 DAYPHN010000000
VERSION     DAYPHN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.293"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..355
                     /locus_tag="ACG0BW_004755"
                     /pseudo
     CDS             1..355
                     /locus_tag="ACG0BW_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            920..2170
                     /gene="ltrA"
                     /locus_tag="ACG0BW_004756"
     CDS             920..2170
                     /gene="ltrA"
                     /locus_tag="ACG0BW_004756"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFK7261597.1"
                     /translation="MTKTKAFNIDKSLVVSAYRRVKTSAGAAGIDKQSLADFDKRLVD
                     NLYKIWNRLSSGSYFPPAVKAVAIPKKLGGERILGIPTVSDRIAQTVVKLAFEPQVEP
                     HFLADSYGYRPNKSALDAIGVTRKRCWYYDWVLEFDIKGLFDNIPHELIMKAVDKHNP
                     ARWVKLYIQRWLTAPMVMSDGEVRARTMGTPQGGVISPLLANLFMHYVFDKWLAKYYP
                     KVPWYRYADDGILHCHSEAEATEMREVLRKRFSECGLEMHPEKTRVIYCKDGSRKGDY
                     EHTMFDFLGYTFRRRVVKNVKRNSLFVSFTPAASKSALKAMRREIKATGIRKRVDLSI
                     EQIAKWINPKLNGWINYYGRYTCSELYSVFRYINKALVRWGRKKYKMLSRYKTRASKF
                     LEEMAKRSPQLFAHWRLKMRGGLV"
BASE COUNT          676 a          436 c          646 g          488 t
ORIGIN      
        1 aggaaatcag gggcggctta ataaagcctt tgaggaaaaa ttcccactga aagaattaaa
       61 taatccagag catgacagtt acgcaatgag tgaaaagagt cacggcagag aagaaatccg
      121 tcttcatatt gtttgcgatg tccctgatga acttattgat ttcacgtttg aatggaaagg
      181 actgaagaaa ttatgcgtgg cagtctcctt tcggtccata atagcagaac aaaagaaaga
      241 gccagaaatg acggtcagat attatatcag ttctgctgat ttaaccgcag agaagttcgc
      301 cacagcaatc cgaaaccact ggcacgtgga gaataagctg cactggcgtc tggacggtgt
      361 gccgcaggaa cacaagatag ctgcataagg atacgcagtt agcttttgta taagctcata
      421 aagagatggt ggaaggccca ccgggatcgg ctgtcggaag cagattccaa accacttcat
      481 gctgctgtgg taaagagcca tggtagtgag cgatgaagta aagcgtgagt gaaaactcag
      541 caggtaagat actcatgaga cgagtgaacc cgaacccatg ttgacgcgtc gtaaaccaga
      601 gactgacatc aaaaccgaag gggcgtccag ccttcgggat aagtctgcga gtgaattccg
      661 aatgctgcgc agacggtgtc cggcgtaaag tgggcgtgac ggagtatcag gctcttcctg
      721 ggaactgcgg gaacctgtcg ttgtgatgat aagggagaaa tgcaagtcac tgaattggcg
      781 agcatgaaag taccgatgca cagcacaggg acggagccgt tcgtagtagt gatgaagctt
      841 ctgtaatgga ggtggagcga aggggcggca tcagacagtc tttggacagt gttcaactga
      901 acatgttcag gaggagacaa tgacaaagac aaaagcgttt aacattgata agtcactggt
      961 ggtcagcgcg tacaggcgag tgaagaccag tgcaggcgcg gcgggaattg ataagcaatc
     1021 gctggcggac tttgataaac ggttagttga taatctgtac aaaatctgga accgactttc
     1081 ttcaggaagt tatttcccac cggcagtgaa agcggtggca attccgaaga aattgggtgg
     1141 agagagaatt ctgggtatcc cgacagtcag cgatcgtatc gcgcagacgg tggtaaaact
     1201 ggcctttgag ccacaggttg agccacattt tctggcagac tcttacggct acagaccgaa
     1261 taaatcggca ctggatgcta taggggtaac gcgtaagcgt tgctggtatt acgactgggt
     1321 actggaattt gacatcaaag ggctgttcga taacatcccg cacgaactga taatgaaagc
     1381 cgtggacaaa cacaatccgg cccggtgggt aaaattgtat atacagcggt ggctgacagc
     1441 gccgatggtg atgtcagacg gagaagtcag agcacgaaca atgggaacgc cgcagggagg
     1501 tgtcataagt ccgttgctgg caaacctgtt catgcattat gtgtttgata aatggctggc
     1561 aaaatattat ccgaaagtgc cgtggtatcg atatgctgat gatggcattt tgcattgtca
     1621 cagcgaagca gaagcgactg aaatgcgaga agtattacgc aaaagattta gcgaatgcgg
     1681 actggaaatg catccggaga aaacgcgagt tatctactgc aaagatggaa gccggaaggg
     1741 tgattatgaa cataccatgt tcgactttct ggggtacaca ttcaggcgac gcgtagtaaa
     1801 gaacgttaag cgaaacagtc tgtttgtaag ttttaccccc gcagcgagca aatcagcgct
     1861 aaaggcaatg cgtcgggaaa tcaaagcgac agggataagg aaaagagtgg atttaagcat
     1921 tgagcagata gcgaaatgga taaatccaaa actgaatggc tggataaatt attacgggcg
     1981 atatacctgt tcggaattat acagcgtgtt cagatatatc aataaagcac tggtgaggtg
     2041 ggggcgtaag aaatataaaa tgctcagccg atacaagaca agagcctcaa aatttctgga
     2101 agaaatggcg aagcgaagtc cacagttgtt tgcccactgg cgtctgaaga tgagaggcgg
     2161 acttgtttga tgggagcggt atgagccgag aggttcacgt accgttctgc gagaggctgc
     2221 gggggaggtt cctgcggcct actcac
//
LOCUS       DAYPHN010000290         2244 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000290 DAYPHN010000000
VERSION     DAYPHN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.294"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..491
                     /gene="paoA"
                     /locus_tag="ACG0BW_004757"
     CDS             <1..491
                     /gene="paoA"
                     /locus_tag="ACG0BW_004757"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFK7261598.1"
                     /translation="NGKTEQLEVDTRTTLLDALRENLHLFGTKKGCDHGQCGACTVLV
                     NGRRLNACLTLAVMHQGAEITTIEGLGSPENLHPMQAAFIKHDGFQCGYCTSGQICSS
                     VAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILAAIEDAAGEI
                     KS"
     gene            488..1444
                     /gene="paoB"
                     /locus_tag="ACG0BW_004758"
     CDS             488..1444
                     /gene="paoB"
                     /locus_tag="ACG0BW_004758"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFK7261599.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAANAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            1441..>2244
                     /gene="paoC"
                     /locus_tag="ACG0BW_004759"
     CDS             1441..>2244
                     /gene="paoC"
                     /locus_tag="ACG0BW_004759"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFK7261600.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWYEE
                     APNAAYGYIVGSAIAKGCLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLAG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKV"
BASE COUNT          493 a          662 c          657 g          432 t
ORIGIN      
        1 tgaacggcaa aaccgagcag cttgaggtgg atacccgaac cacgctactg gacgctttgc
       61 gtgaaaatct gcatctgttc ggtaccaaga aaggttgcga tcacggacag tgcggagcct
      121 gtaccgtgct ggtcaatggt cgcaggctta atgcctgcct gacgcttgca gtcatgcatc
      181 agggggccga gatcaccacc attgaaggcc tgggttcacc agagaatctt caccccatgc
      241 aggcggcctt tatcaagcat gatggcttcc agtgcggcta ctgcacctcc gggcaaattt
      301 gctcatcagt agcggtgcta aaagagattc aggacggcat tcccagtcac gtcacggtcg
      361 atttggtttc ccctccagaa agaactgccg atgagatccg tgaacgtatg agcggcaaca
      421 tctgtcgctg tggagcatac gctaacatcc ttgccgccat tgaagacgct gcgggggaga
      481 taaaatcatg aaggcgttta cctatgaacg agtgaacacc ccggccgagg cggcacttag
      541 cgctcagcgc gtacccggcg caaaatttat cgcgggcggg accaatctgc tggacctgat
      601 gaagctggaa attgaaacgc ccacccacct tatcgatgtg aacggcctcg ggctcgataa
      661 gattgaagtg accgacgcgg gtgggctgcg catcggcgca ctggtacgga acaccgacct
      721 ggcggctcac gagcgcgtgc gtcgtgatta cgcggtactc tcccgcgccc tgctcgctgg
      781 cgcgtctggt cagttacgta atcaggcaac caccgcaggt aatctgctcc agcgcacgcg
      841 ctgcccctat ttttacgaca ccaatcagcc ctgcaataag cgcctgcccg ggagcggctg
      901 cgcggcactt gaaggcttta gccgtcagca cgcggtggta ggcgtaagcg aagcctgcat
      961 tgccacccat ccgagcgata tggcggtcgc aatgcggttg ctggatgcgg tggtggaaac
     1021 catcacgccg gagggaaaga ctcgcagtat cacactggct gatttttatc acccaccggg
     1081 gaaaacgccg cacattgaaa ccgccctgct tcccggtgag cttatcgttg cggtgacgtt
     1141 acctccgccg ctcggcggaa aacatattta ccgtaaggtg cgcgatcgcg cctcctacgc
     1201 ctttgccctg gtatcggtcg cggcgattat tcagcctgac ggcagcgggc gcgtcgcgct
     1261 gggcggagta gcacataagc cctggcgcat tgaggctgcg aatgctcagc tatcccaggg
     1321 ggcgcaggcc gtgtatgacg cgctgttcgc cagcgcccat cccaccgctg aaaacacctt
     1381 taaactcctg ttggcgaagc gaacgcttgc ctccgtactg gctgaagcga gggcacaggc
     1441 atgaaatttg ataaacccgc aggggaaaac ccgatcgatc agctgaaggt tgtcggtcgt
     1501 ccccatgacc gcatcgacgg accgctgaaa actaccggca cggcacgcta cgcctacgaa
     1561 tggtatgaag aagctcccaa cgccgcctat ggttatatcg tcggttccgc cattgccaaa
     1621 ggatgcctca ccgcccttga tacggacgcc gcgcaaaaag cgccgggcgt actggctgtc
     1681 attaccgcca gtaacgccgg ggcactcggc aaaggcgaca aaaacaccgc caggctgtta
     1741 gccggcccca ctattgagca ctatcatcag gccattgcgc tggtagtggc cgagaccttc
     1801 gaacaggcac gagcggcggc ctcgctggtg caggcgcact atcgccgtaa taaaggagct
     1861 tactccctgg cggacgagaa acaggccgtc agtcagccgc cggaagacac gcccgataaa
     1921 aatgttggtg actttgacgg ggctttcacc tccgctgcgg tgaagattga tgctacctac
     1981 acgaccccgg accagagcca tatggcgatg gagccgcatg cctcgatggc cgtctgggat
     2041 ggaaataagc ttaccctctg gacctcaaat cagatgattg actggtgccg caccgatctg
     2101 gcaaaaacgc tgaaagtgcc cgtggagaat gtgcgtatta tctccccgta tatcggcgga
     2161 gggtttggcg gcaagctgtt cctgagaagc gatgcgctgc tggcggccct cgccgcccgt
     2221 gcggtgaaac gtccggttaa agtg
//
LOCUS       DAYPHN010000291         2211 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000291 DAYPHN010000000
VERSION     DAYPHN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.296"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            14..1036
                     /locus_tag="ACG0BW_004760"
     CDS             14..1036
                     /locus_tag="ACG0BW_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFK7261601.1"
                     /translation="MPDDIKTLKSDVSKLKKDQGSYATKSYVDNKTTWNGYCNVIYDQ
                     KTLPTTGTIFSGKIHLSNKTGETENAYGEMYTRKNIDGTKDTMTRIVTHNGTKGIFWD
                     FSDLYGGTLIFPGSDGYLKMGNCLMSYGVRGSNALIKFDCTDTLQIKYANHGSTMTIN
                     TQGTAHAGVTASLWGNSSRPVVYEVGADGGAYMFYAQKHTDNTYMLSVNGACHATAFN
                     QHSDRDLKDNIQVIDNATDRIRKMNGYTYTLKENGIPYAGVIAQEALEAIPEVVGSAM
                     KYQDGGSGSKGEEGERYYTVDYSGVTGLLVQVARESDDRITALEEENAELRQRLSAIE
                     VALASK"
     gene            1079..1372
                     /locus_tag="ACG0BW_004761"
     CDS             1079..1372
                     /locus_tag="ACG0BW_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261602.1"
                     /translation="MDITPFLHALCAVAAQVLVGLFTGNWVYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDLRAWDVASLMDFAVPVVACLLVWLLVNRV"
     gene            1392..1502
                     /locus_tag="ACG0BW_004762"
                     /pseudo
     CDS             1392..1502
                     /locus_tag="ACG0BW_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1600..2190)
                     /locus_tag="ACG0BW_004763"
     CDS             complement(1600..2190)
                     /locus_tag="ACG0BW_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFK7261603.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          635 a          441 c          534 g          601 t
ORIGIN      
        1 gaacttaaca gtaattcctg atgacatcaa aacgctaaaa tccgacgtta gcaaattaaa
       61 aaaagatcaa ggaagctacg caacaaaatc atatgtagac aataaaacaa catggaatgg
      121 ttattgcaat gtaatctatg atcaaaagac attgccgacc actggaacta tatttagcgg
      181 taagattcac ttgtcaaata agacaggaga aaccgaaaac gcttatggtg agatgtacac
      241 cagaaaaaat attgacggta caaaagatac aatgacaagg attgtcacac acaatggaac
      301 aaaaggtatc ttttgggatt ttagcgatct ttacggtgga acattaattt ttccaggcag
      361 tgatggttac cttaagatgg ggaactgtct catgtcgtat ggtgtgcggg gaagtaacgc
      421 gcttattaag tttgactgca cagacacatt acaaatcaaa tatgctaatc atgggtcaac
      481 catgacaata aacacacaag gaaccgctca cgctggcgtt actgctagct tgtggggcaa
      541 ctctagccgt ccggttgtat atgaagttgg tgctgatggt ggcgcgtata tgttctatgc
      601 gcagaaacat accgataaca cctatatgtt aagcgttaat ggtgcatgtc atgccaccgc
      661 atttaaccag cattccgacc gggatctgaa agacaacatt caggtgatcg ataatgcaac
      721 cgaccgcatc cgtaaaatga acggctatac atacactctt aaagaaaatg gcatacctta
      781 cgctggtgtt atcgcgcaag aagcactaga agcaatccca gaagttgtag gctctgcaat
      841 gaaatatcag gatggtggaa gcgggtcgaa aggtgaagaa ggtgaacgtt attacacagt
      901 agattattct ggtgttactg gcttgcttgt tcaggtagcc agagagtcag acgacagaat
      961 aacagcactg gaagaagaaa acgcagaatt aagacaaaga ttatctgcaa ttgaggtggc
     1021 gcttgcgtct aaataatatt aaggggccga gcgccccgtt ttattgggtg ggatgaaaat
     1081 ggatataaca cctttccttc atgcactttg tgctgtggct gcgcaggtac tggttggcct
     1141 ttttaccgga aactgggttt acggtgcgat agccggttgt acgttcttca ttgcgcgtga
     1201 acacacccag gcagaatatc gctggattga aatgttcggg catggcaagc gaatgaatat
     1261 gccgtggtgg ggcggttttg atctacgcgc gtgggatgta gcaagcctga tggattttgc
     1321 tgtgccggtg gtggcgtgtc tgctggtctg gctgttggtt aatcgtgtgt gaaaaaaggt
     1381 gagctgtata tgcaacggag gaagaaaccc cgttgctgga agcatggaaa aagtatcggg
     1441 tattgctgaa ccgtgttgat acgtcaactg cacaggatat tgaatggcca gcactgccgt
     1501 agggtaaaac atataaattc tataattaga tgtatctttc catttacggc aaggaagggg
     1561 gcttggaaga cgtaaagcat ctcacaccga gattattttt tatatgtcag gtgtctgaag
     1621 ttttgctttg gctcttaaaa tggtttgccg cgaggttttg aattcccggg caatggcact
     1681 tatacttaca cctgacttaa ttcgttcgaa taccacctgt ttctgttctt catttaacac
     1741 aggtggtcga ccaaaacgtt tccctgcgcc gcgggctctt actatcccgg aatgagtgcg
     1801 ttcaagtaaa aggtctcgtt caaattcagc gactgctgaa attacttgca tcatcatttt
     1861 tcctgttgga ctggtcaggt caatgccacc caatgctaag caatgcactc tgatacctgt
     1921 ttcggtcagt tgttccactg ttttcctgat atccattgca ttacaaccaa ggcgatccag
     1981 ttttgtcaca atcaattgat caccacattt caggcgagca agcaaccggt taaaaccagg
     2041 acgctcactg gttgctgctg agccgctaat gtgttcttcg attatttgct gaggtttgat
     2101 tttaaaacct gcactttcga tttcccggcg ttgattttcg gtggtctgat ccagcgttga
     2161 tatccgacag taagcaaaaa ttcgagacat agtgagactc tatacgaaat t
//
LOCUS       DAYPHN010000292         2202 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000292 DAYPHN010000000
VERSION     DAYPHN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.297"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..365)
                     /locus_tag="ACG0BW_004764"
     CDS             complement(<1..365)
                     /locus_tag="ACG0BW_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFK7261604.1"
                     /translation="MANKTEKTTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAIKFKFDSLNPVNG"
     gene            complement(374..1141)
                     /gene="sctT"
                     /locus_tag="ACG0BW_004765"
     CDS             complement(374..1141)
                     /gene="sctT"
                     /locus_tag="ACG0BW_004765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFK7261605.1"
                     /translation="MGEAMLYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDVALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFQEQLQLYIDK"
     gene            complement(1143..1403)
                     /locus_tag="ACG0BW_004766"
     CDS             complement(1143..1403)
                     /locus_tag="ACG0BW_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFK7261606.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1414..2079)
                     /locus_tag="ACG0BW_004767"
     CDS             complement(1414..2079)
                     /locus_tag="ACG0BW_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFK7261607.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2069..>2202)
                     /locus_tag="ACG0BW_004768"
     CDS             complement(2069..>2202)
                     /locus_tag="ACG0BW_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFK7261608.1"
                     /translation="PEAEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT          764 a          459 c          348 g          631 t
ORIGIN      
        1 aagccattta ctggatttaa tgaatcaaac ttaaacttaa tagcttcagt tgctaattgc
       61 attttacttt gaaaccatga aacaagtatt gttgtaaaca tacaaaccag caaaaaagga
      121 atgatcgttt tcaaccaacc tatccccata gctttaaaat agtcccaaat atccggttta
      181 gcgttatgat caaggatgta ttcaagaatc gacataatgt gatgcacatc aaatacatat
      241 ccgagatata aagtacccac aagcattatt acagagactg ttaagtctct actttttaga
      301 atttgacctt ttttagaagc atcctggagt ttcttttgtg ttgtcttttc agttttattt
      361 gccatgaaac actctattta tcaatgtata attgcaattg ttcttggaaa aaggataacg
      421 gcataactcg ttcagccagt attgggccaa aataaataat taatattaaa aatgccagcg
      481 cacttttgac agtcagtgag atggcaaaag catttagttg tgaagcataa cgtgccagca
      541 atcccaggac agcttcaccg cccagcataa ccgcaatcac ggggcttgcg taaacgatag
      601 tatgagtcat tatattatta atatatgaga ataaaggctc taatttagga aaattaaaat
      661 ttcctgaagg ccataagtta taactttgcc atagagtctc cagaataaag ttcaatccac
      721 cgttagtaag ataaactgct gcagagaaaa ggttaaataa tcttgctaat tccgatgtat
      781 cgacacctgt agccggatcc aaagtactac ttaacgttgc accacgttgg ttatcgataa
      841 agctacctat agcaaggaaa atccaaaatg gggatgctaa taagcaagca ataaacaacc
      901 ctatgataat ttctctggca gcaaacaaag cgacatctat ttcatttagg gtagataatt
      961 catagtggta atgctgaact aatgcacatg ataccactat tattattgga tgacggacta
     1021 ctgttggtat attcccactg gcgaaaaatg gtaacaaata gaaagttggt gccaatcggc
     1081 aaaaaccgag cgctgtagca gccagcagac tatgcaactg atagagcatc gcctccccca
     1141 tgctatcctc tcgcgaatgc taaattcagc atttcgatac caaacgaata taatttttca
     1201 ccataccatc cgctcattaa aaaaaagcaa atacttacgc acaataattt gacgccaaat
     1261 ggcaacgtct gttcttgcaa ctgtgttacc gtttgaaaaa gccctaccaa taaaccaacg
     1321 aatgtcgcaa ctgcaatagg tcctgcagac ataactaaaa tcagatacag cgccctattc
     1381 cccgcaaaaa caatgtcgtc catatttttt tcctcaggga ttaatggata ggtcaaaata
     1441 ttgtaaaatg agtcctttcg ataacattgt ccaaccgtcc atcgcgacaa acagaattaa
     1501 ttttatcggt gtagatatgg ttaccggact catcatcatc atacctaacg ttaacagaac
     1561 gctggatata accagatcaa ctacaacaaa gggtaaataa atataaaaac ctataataaa
     1621 tgcacttttt atctcactca acgcatatgc cgggagtaac gagaaaattg aaatattatc
     1681 atcatcgatt atctcttcat tgtcttcaga actattcacc ttctgaatct tttcaaaaaa
     1741 gctaaccaac tccggttcag aatattttat caaatatgat ttataaccac tcatgcctgt
     1801 ctcaacaaaa ttaacaaccg acgcaacatt attgaatgaa agattctcat tctgactgtt
     1861 attatatatt tccttcccaa ccggcatcat tacgaacatt gacaatagca aagctacccc
     1921 attaagtgtc atatttgatg gcacttgctg aagtcccaaa gcgtttcgta caataacaaa
     1981 aacaatcgag aatttaataa aacatgtccc ggatgctatt ataaacggta atagtgtaaa
     2041 taatgataat atcgctatca gcgaaatgct atttgacatt attattccct gataaccaag
     2101 aatgaatttc tacgcccaat ttatcatcca cttcaacaag ttcgccatag ccagttagcg
     2161 cgccatttac acgtatctct atatttttct ccgcttcagg aa
//
LOCUS       DAYPHN010000293         2178 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000293 DAYPHN010000000
VERSION     DAYPHN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.298"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            305..1177
                     /locus_tag="ACG0BW_004769"
     CDS             305..1177
                     /locus_tag="ACG0BW_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFK7261609.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            1505..>2178
                     /locus_tag="ACG0BW_004770"
     CDS             1505..>2178
                     /locus_tag="ACG0BW_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFK7261610.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIV"
BASE COUNT          519 a          584 c          585 g          490 t
ORIGIN      
        1 tcccactgac atctccccca caatacactg aaaaatactc acagcagtaa acacagacac
       61 acctgataat catgcagccg ataaatattt tcccctttac ggagaaatca gcctgctgtt
      121 taacaataac ggcgtatttt catggaagga gatcacccag taacggataa gtcctgatct
      181 ccttcatgtt aatacgcaac aatcccatcg attaaaaaca atttcagaca gatattatct
      241 ccgtgaatcc cccgccacct ttccggtgcg cggggttttg tcttttttca ccgggaatac
      301 atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct ccctgcctta
      361 ctcgctttcc cgtcacatcc tggaacatct gcgcaaactc acccgtcacg aacccgtgat
      421 tggcattatg ggtaaaagcg gggccggtaa atcctcactc tgtaatgcac tgtttcaggg
      481 ggaggtcacc ccggtcagtg atgttcacgc cggcacccgg gaagtacggc gcttccgtct
      541 gagtggccac ggtcacagca tggttatcac tgacctgccc ggggtgggcg agagccggga
      601 cagggatgca gagtatgagg ccctgtaccg tgacattctg cctgaacttg acctggtact
      661 gtggctgatt aaagccgatg accgtgccct gtctgtggat gagtatttct ggcgacacat
      721 cctgcaccgg ggacatcagc gggtactgtt tgtggtgacg caggccgaca aaacggagcc
      781 ctgccatgaa tgggatatgg ccggtattca gccttctcct gcacaggcac agaatattcg
      841 cgaaaaaacg gaggcggtat tccgtctgtt ccggcccgta catccggttg tggccgtatc
      901 ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg ctcatgacag cgcttcccga
      961 ccatgccgcc agtcccctga tgacccgact gcaggacgag ctgcgcacgg aatctgtccg
     1021 ctctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca cagcggagag
     1081 cgtctgtgtt gcctctgttg tccgtacggc cctgcgcgct gttcgtgaca cggtggtctc
     1141 tgttgcccgt gcggtatgga actggatttt cttctgaacc tgtcgtgact gatgccctcc
     1201 ctgactctga gtctgctcac aaaagcactg ttttcgttac tgtctctctt gtccgtgcaa
     1261 tagctcaata atagaataaa acgatcgata tctattttat cgatcgttta tatcgatcga
     1321 taagctaata ataacctttg tcagtaacat gcacagatac gtacagaaag acattcaggg
     1381 aacaacagaa ccacaattca gaaactccca cagccggacc tccggcactg taacccttta
     1441 cctgccggta tccacgtttg tgggtaccgg cttttttatt caccctcaat ctaaggaaaa
     1501 gctgatgaaa cgacatctga atacctgcta caggctggta tggaatcaca ttacgggcgc
     1561 tttcgtggtt gcctccgaac tggcccgcgc acggggtaaa cgtggcggtg tggcggttgc
     1621 actgtctctt gccgcagtca cgtcactccc ggtgctggct gctgacatcg ttgtgcaccc
     1681 gggagaaacc gtgaacggcg gaacactggc aaatcatgac aaccagattg tcttcggtac
     1741 gaccaacgga atgaccatca gtaccgggct ggagtatggg ccggataacg aggccaatac
     1801 cggcgggcaa tgggtacagg atggcggaac agccaacaaa acgactgtca ccagtggtgg
     1861 tcttcagaga gtgaaccccg gtggaagtgt ctcagacacg gttatcagtg ccggaggcgg
     1921 acagagcctt cagggacggg cagtgaacac cacgctgaat ggtggcgaac agtggatgca
     1981 tgagggggcg atagccacag gaaccgtcat taatgataag ggctggcagg tcgtcaagcc
     2041 cggtacagtg gcaacggata ccgttgttaa taccggggcg gaagggggac cggatgcaga
     2101 aaacggtgat accgggcagt ttgttcgcgg ggatgccgta cgcacaacca tcaataaaaa
     2161 cggtcgccag attgtgag
//
LOCUS       DAYPHN010000294         2168 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000294 DAYPHN010000000
VERSION     DAYPHN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.299"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..558)
                     /locus_tag="ACG0BW_004771"
     CDS             complement(<1..558)
                     /locus_tag="ACG0BW_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261611.1"
                     /translation="MFGMSITQSDGSLWMSPEFTPQNLINKGTMSTSKGSVFQTSIPS
                     NKSCFFFIKSSNKANMMFIHEHSNGYNALRLHQVNGSPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGAMVYHGEMMPLDAKLITISDPQFTIDVGYPCAVMPAMVGVYNYRRTDYDRP
                     VYVTMTGATGNQVYNGQWYSGNVTWD"
     gene            complement(561..842)
                     /locus_tag="ACG0BW_004772"
     CDS             complement(561..842)
                     /locus_tag="ACG0BW_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261612.1"
                     /translation="MAYGTGIYNNKGINVTGFLTPIFFLDRFTASSGSKTYSNPPPGK
                     SLHAVWSLMPLNNDNYINLPVPTVTINGNTVSWSNLYTGLGSYIYTYWG"
     gene            complement(898..1467)
                     /locus_tag="ACG0BW_004773"
     CDS             complement(898..1467)
                     /locus_tag="ACG0BW_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261613.1"
                     /translation="MCKKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKERADKLQATLDMVSETQRTDDTNKSEAI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(1607..1765)
                     /locus_tag="ACG0BW_004774"
     CDS             complement(1607..1765)
                     /locus_tag="ACG0BW_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFK7261614.1"
                     /translation="MLERFRLVIVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKAKC"
     gene            complement(1765..>2168)
                     /locus_tag="ACG0BW_004775"
     CDS             complement(1765..>2168)
                     /locus_tag="ACG0BW_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFK7261615.1"
                     /translation="TRKAVIEVKKMENRDAVEATVWGAYMISYADGTCDAKEIAILEK
                     TIAALPAFSPFAGEIAQMSANIRARYEASPRSANAQAIRELSDIAGTPEAVDVLCLCL
                     DIADQDGIGEQEEQALKKIAQALQLSLDAYL"
BASE COUNT          615 a          497 c          446 g          610 t
ORIGIN      
        1 atcccatgtg acattaccgg aataccattg accgttatat acttggtttc cagttgcacc
       61 ggtcatagtt acatagacag gtctgtcgta atcggttcgt ctatagttat aaaccccaac
      121 catagcaggc attaccgcac acggataccc cacatctatg gtaaattgag ggtccgaaat
      181 agtgataagt ttcgcatcaa ggggcatcat ctcaccgtga tacaccattg cgcctgcgct
      241 gttatacatg gcaatgccat agccagaatg tggtaacacc atatcagaaa aagcataaac
      301 tgttattgtt ccggggctac catttacctg atgcaatctg agagcattgt atccgttact
      361 atgctcatga ataaacatca tgttcgcctt attactgctt tttatgaaga aaaagcatga
      421 tttgtttgat gggattgatg tttgaaaaac agaaccttta gatgtagaca ttgtcccttt
      481 attaatcaga ttttgcggag taaattctgg actcatccac aaacttccat ctgattgagt
      541 aattgacatc ccgaacataa ttatccccag tatgtgtata tgtaagaacc aagccccgta
      601 tataaatttg accaactaac cgtatttcca ttaatagtaa ctgttggaac aggtaaatta
      661 atgtagttgt cgttgttaag aggcatcaat gaccacacgg catgtagcga tttacccggt
      721 ggcggattgg agtacgtctt agagcctgat gacgcagtaa atcgatcaag aaaaaaaata
      781 ggagtaagga agccagtaac gttaattcct ttattgttgt aaatccctgt accgtatgcc
      841 atttttcatc cttgtttttt atcaacacaa tatggccgat catgcggcca tatcagatca
      901 attagagctt tgaaaggctt tgtgtggctg tgttgcgagg ttatgtcgat ggcttaactc
      961 acgcatcatg tcttcaagcc gactctttgt gtcgtcgagt tggtcggcca ttgctcccaa
     1021 aagctgacga acggccatcg gatcatcgct gtttagtgat ggcattttat aacccgcctg
     1081 agaagacata agattgaacg cagacatcaa catcgttaga gaggatttaa gcccagcaat
     1141 ttcacgatct ttgctggcaa taatcgcctc actcttgtta gtgtcatcag ttcgttgagt
     1201 ctcgctcacc atatccaaag tcgcctgcaa cttatcagcg cgctcttttt cagcaagata
     1261 atgaacaccg aaatgatgtg ctaaagctac aacctgagtt ggctcttcaa aggtggactc
     1321 aaaattaagt gctgtaacca ctctctcaaa tatggaaacg tcttctattc ctcgaagaat
     1381 ggctaacatt ttgaccagtt cattagcatc catttcttcg aataaggcgt tttgttcatc
     1441 agctatcgct ttgcatttct tgcacatgtg tttttcctaa tgcttaaaca agttgttttc
     1501 ttattggctt tattgtgatg tggcataaaa ggggagcaag ctaaacgtaa aggtgcaaac
     1561 tctctatttc ggtcaacgaa acacaagggc catttggccc ttgtcattaa cactttgctt
     1621 tacgaactaa cggataagcg aagtagatcg ccaaaccaat gaggacgcca tcagaaatga
     1681 cagacatcat ctttcccgta aaatccacca acaccgccat caccagaaga gcaatgacga
     1741 tcacaagccg gaatctctca agcattagag ataagcatcc agtgacaact gaagcgcctg
     1801 ggcgatcttt ttcagtgcct gctcttcttg ttcaccaatg ccgtcctgat cggcaatatc
     1861 aaggcacaga cacagaacat cgaccgcctc tggagtaccc gcaatatcag acagctcacg
     1921 aatagcctgt gcattagcag aacgagggga cgcttcgtaa cgagcacgga tattggcgct
     1981 catttgggca atttcaccag caaacggaga aaacgcaggc agagctgcaa ttgtcttctc
     2041 aagaatggca atttcttttg cgtcacatgt gccgtcggca taggagatca tgtacgcgcc
     2101 ccacacggtg gcctcaaccg catcacggtt ctccattttt ttgacttcga taacagcttt
     2161 acgagttt
//
LOCUS       DAYPHN010000295         2164 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000295 DAYPHN010000000
VERSION     DAYPHN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.300"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..497)
                     /gene="tetR"
                     /locus_tag="ACG0BW_004776"
     CDS             complement(<1..497)
                     /gene="tetR"
                     /locus_tag="ACG0BW_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR"
                     /protein_id="HFK7261616.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAA"
     gene            603..1802
                     /gene="tet(A)"
                     /locus_tag="ACG0BW_004777"
     CDS             603..1802
                     /gene="tet(A)"
                     /locus_tag="ACG0BW_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFK7261617.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1834..>2164)
                     /locus_tag="ACG0BW_004778"
     CDS             complement(1834..>2164)
                     /locus_tag="ACG0BW_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFK7261618.1"
                     /translation="LLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFRGIS
                     RLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRAR
                     IACRKSP"
BASE COUNT          381 a          674 c          670 g          439 t
ORIGIN      
        1 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccggc ctgctcctca
       61 agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc
      121 gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg
      181 cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg
      241 aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa
      301 tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc
      361 ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc
      421 gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc
      481 ggctgcaact ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact
      541 tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag
      601 acgtgaaacc caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg
      661 gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg
      721 acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg
      781 cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc
      841 tggccggcgc tgctgtcgac tacgccatca tggcgacggc gcctttcctt tgggttctct
      901 atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata
      961 ttgccgatat cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt
     1021 tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc
     1081 acgctccgtt cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc
     1141 ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc
     1201 tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct
     1261 tcatcatgca acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc
     1321 gctttcactg ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt
     1381 cactcgccca ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac
     1441 tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg
     1501 gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc
     1561 tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac
     1621 tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg
     1681 cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc
     1741 tctgcctgcc ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct
     1801 gatcgtggaa acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg
     1861 cgccctagga gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac
     1921 gccgatgatt tgaagcgcac tcagcgtctg atccaagaac aaccatccta gcaacacggc
     1981 ggtccccggg ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc
     2041 ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag
     2101 aacattggtt cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag
     2161 caga
//
LOCUS       DAYPHN010000296         2157 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000296 DAYPHN010000000
VERSION     DAYPHN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.301"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(158..586)
                     /locus_tag="ACG0BW_004779"
     CDS             complement(158..586)
                     /locus_tag="ACG0BW_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261619.1"
                     /translation="MTKTQAKLLIYKANKFMQKLDGIDSFSITLLYDNSSLMRKEFHQ
                     THNIDCLNDRLFLPHPVNINLLISQIVTLSGTAECFRCWLIYEGKVKIVINGNNFTSF
                     AQSIFREQGTYDLTLFFDITRRVIVVCDNEYDIDIYTTTY"
     gene            complement(614..1060)
                     /locus_tag="ACG0BW_004780"
     CDS             complement(614..1060)
                     /locus_tag="ACG0BW_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261620.1"
                     /translation="MNMTKKEALAFLALNQPMPNDYDITQELINKYNNVRLYFSANPA
                     EEAIPLFLQSFGEGDGFGVYQLVEDFLYKCDKNIIASNIANILENPLTIKSVRCWCTL
                     LAMAFPDNTLIKGLNISLQSDDEDTRDMAMLSLKMITEEYKTFEFQ"
     gene            complement(1881..>2157)
                     /locus_tag="ACG0BW_004781"
     CDS             complement(1881..>2157)
                     /locus_tag="ACG0BW_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFK7261621.1"
                     /translation="ADLTAEKFATAIRNRWHVENKLHWRLDVVMNEDDCKIRRGNAAE
                     LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          657 a          383 c          383 g          734 t
ORIGIN      
        1 gatggtaatt attctgtata gcatgcatgc taacaggcca atcagtagtt tgttcgcaaa
       61 tttataaggg gattcgctat tggttgattg catggttatt atgctcttga attgtgtatt
      121 ttattggaat gtttcttggt taactaaaac aatgcattta ataggtggtg gtgtaaatat
      181 caatgtcata ctcattatca cagactacta ttactctacg agttatgtca aaaaataacg
      241 ttaaatcata ggttccctgt tctctgaaaa tactctgcgc aaatgacgtg aaattattcc
      301 catttataac tatttttact ttaccttcat agataagcca gcaacgaaaa cattcagctg
      361 taccagataa tgtcactatc tgactgatta gaagatttat atttaccgga tgaggtaaaa
      421 aaagtctgtc gttgaggcaa tcaatattat gtgtttgatg aaactctttt ctcattaatg
      481 aagagttatc atatagcagg gttatactaa acgagtcaat tccgtcaagc ttttgcataa
      541 atttgtttgc tttataaata agcaactttg cttgtgtttt tgtcatgatg gtgatctttt
      601 atgcaagggg gaattattgg aattcaaatg ttttatattc ctctgtaatc atctttaaac
      661 ttaacatagc catgtctcgg gtatcttcat catcagattg tagagaaata tttaatcctt
      721 taattaaggt gttatcggga aaagccattg ccaataatgt acaccaacat cggacacttt
      781 ttattgtcag aggattttcc agtatgttgg cgatatttga agctataatg tttttatcac
      841 acttgtacaa aaaatcttcg accaactgat atacaccgaa gccatcacct tccccaaacg
      901 attgtaaaaa taacggtatg gcttcttcag caggatttgc agaaaaataa agccttacat
      961 tgttatattt attgataagt tcctgagtga tgtcataatc atttggcata ggttggttta
     1021 gagcaaggaa tgctaatgct tcttttttag tcatattcat aatggttaat attgcttgct
     1081 ggaagaatta aggtaaaaat ggttcataga cagaattaaa taggaagtct tgtttccaga
     1141 taactttatc atgaaaaaga gatttggctt gatgcgtata aagattatga ggataaatag
     1201 tttctccacc ataaatgatg ttctttcccg ttacaccgga gaattcattg gctataatct
     1261 gtcgtttaag ccagtgaatg actttacata agcatattaa tttatcctta gaatcagttg
     1321 tgttaatttc agtaagttgt aaaatctttt ccccattata ctcaactaaa tgccccggct
     1381 ttatttcaat tcaaccacaa taacgatcaa tgctactaaa ctttattctt ctattgttag
     1441 gtttaatgct gggcataaga taaattcgat gtgctacggc agggatcttt tctgttaaga
     1501 catttaattg cttagagtat tcatcttgta gtgtaaacca aattgaatgt aatttgtatt
     1561 ttgaggctac aatcgcgagc caattagaca gttcctcgtt cgtgcaaaaa acatttatcc
     1621 tggcagttgc cattcgtaat cctttttata taagattgtg tgaatgtcac cgaaacaact
     1681 tgatatgaat aaaatagcta aagcgaaatg gatgacgtgt tactaaaaaa ccattctgaa
     1741 aaggaagcct gaataaacgc gatgacgaaa cttccactct ccatgtttca tcagaaaaat
     1801 aactaaattc gatgtggttt tcatataatc tcatttcacg taaggttccg ctaccataag
     1861 ggaccgcaat cagggaaaga ttacgaaagc ccgctcccag caaggactga cgccagatag
     1921 tttctgtcca tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca
     1981 ttcgtcaaaa tattaatagc gatgtgccgt atccctgaaa ataattctgc ggcgtttcct
     2041 cttcttattt tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc
     2101 tccacgtgcc agcggtttcg gattgctgtg gcgaacttct ctgcggttaa atcagca
//
LOCUS       DAYPHN010000297         2150 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000297 DAYPHN010000000
VERSION     DAYPHN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.302"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            48..293
                     /locus_tag="ACG0BW_004782"
     CDS             48..293
                     /locus_tag="ACG0BW_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HFK7261622.1"
                     /translation="MTITIYGRDNCSYCKRAVELAKQLKGHGYGDYEYIDITTAGIDK
                     EKLSEIVGKPVETIPQVLIDGQPIGGYTELAAYVSTL"
     gene            504..1607
                     /locus_tag="ACG0BW_004783"
     CDS             504..1607
                     /locus_tag="ACG0BW_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFK7261623.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLQRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKGSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(1602..1988)
                     /locus_tag="ACG0BW_004784"
     CDS             complement(1602..1988)
                     /locus_tag="ACG0BW_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFK7261624.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIAKTIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
BASE COUNT          615 a          470 c          493 g          572 t
ORIGIN      
        1 ctatcttctt tgtaagtaaa gttgcatatc aattctatga ggtaacaatg acgatcacta
       61 tctacggacg agataactgc tcatactgca aacgtgcggt cgagctggcg aagcaactaa
      121 agggacatgg ctacggtgat tatgagtaca tcgacatcac cactgccggt atcgacaagg
      181 aaaaactaag tgaaattgtt ggtaaaccgg tagagactat cccccaagtg ctgatcgatg
      241 gccaaccgat tggcggatac acagaactgg ctgcatacgt cagcaccctc tgattttaac
      301 ggctcacagg agccgttttt attcccacca aactcactcc cgtttccctt aaaattcaaa
      361 aaacaacgtc aaaatgattc catacctact atgtatggaa tcattactga aaatgagtta
      421 ctttgactct tgatcctata agaatctatg cctaatatac tgtttactta tacagtgaat
      481 cggcgtaact cggtgattgt catatgaaaa atagctttga cagagcacgc gctgcggaga
      541 acacctcaaa agaggcgatt gagtatctcg aaagagcatc tcaaatgcag gccgttatga
      601 tctcgcaggt tagcaatgat atgagattct cggacgcatt catgttattt actcgcttat
      661 ctctgctgat aacaagacgt cggccggaga tcgctgttca ttgtattttg atacatgttt
      721 tgccgcacat tgctgatgta aaagtaagtg acattaatag gttcatggtg aaccaactgg
      781 tcaaccccct aatactggat ggcaaaattg ttatgggccg ccgcgttttc tctctgatga
      841 agcagttcct tagctggtgt gccttccagg ggatgatcga cgtgtcaccg ttaaacgata
      901 tgtcactaaa caaagttgcc ggtggcgcaa agcccacgcc tcgcgagcgg aagctaaccg
      961 acgcagaggt atgggtgttc tggaatatat gggactactt caatgtgtgc gctggtacaa
     1021 aatgggcggc caggctatgt cttgtatccg caagacgacc tgacgaagta ctgcgggcta
     1081 aaaaaagtga gttcaacctc cagcgtgggg tttggaatca aggcaagaga aacaaatcag
     1141 cccgtgagca ttctctgcct ttaagcacat taatgcgcac atgcattgaa gagttattcg
     1201 aatatggtaa aggcagccag tggcttgtgc cttcgaataa aaaaatcggg aaagatcttc
     1261 cgatgtctaa agtggcaata gcccaggcat tacgccgtat cctggaacga ccagaactga
     1321 tggagcttga gccatttaca ccgcgagatt tgcgtcgtac tgcgcgtagt tacttcccag
     1381 cattaggcat aagccaggag gtatcacgta aaatcatgaa ccacagtctt gaggggatag
     1441 atcgggtcta cgaccggcac gattatatgg acgagatgcg agacgcctta gaaagtttct
     1501 cgacgtacat cgcatcaatc gtagagcaac cggatttaga cgaaattgac cacaaattca
     1561 agggagatcg tctatcgaca gagcttattc gtgtaaattt ttcatagaga ctttatggcc
     1621 tcaacaacct tttgtgatgc gcctttctct ttaccgaatc gctcgttata tgcagcaaga
     1681 acctgttttt cgtcctcgtt aagaggagca gtgccttctt tgtataaaaa tgctgcgagt
     1741 tcgggttggc gttcttccag caccatcatc ataagacgac ttggctcaat acccagtgcc
     1801 agcgccagcg gacgaacctt atcgataggc aaaggaattt tgccgctttt aattaaagaa
     1861 aggttgttgg cgtttttata cccaattgtt ttggctatct gggcctggct cataggtgag
     1921 gattcaatca accctgcgat aaaagcagcg tagcgacttt ctataaattc aatcttgttg
     1981 tcagacattg ttacaacctt tgcgcgttca attctctctg gtaagtgctt accgatatta
     2041 catcaaaggt tagggttgta aagctattat catttttttc gataggcact taaaagaccg
     2101 gttaaaggcc attgcacgga gaaaaaatta gcccaaaaca ggtaagaaaa
//
LOCUS       DAYPHN010000298         2142 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000298 DAYPHN010000000
VERSION     DAYPHN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.303"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1227
                     /locus_tag="ACG0BW_004785"
     CDS             <1..1227
                     /locus_tag="ACG0BW_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFK7261625.1"
                     /translation="GRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAF
                     VQGNRLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSH
                     QTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMS
                     VTAGVYGAAGHSSVDVKDDDGSRVGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRH
                     SMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGY
                     VKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIR
                     TFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSG
                     SSAEGYNGQATLNVTF"
     gene            1348..>2142
                     /locus_tag="ACG0BW_004786"
     CDS             1348..>2142
                     /locus_tag="ACG0BW_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261626.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERN"
BASE COUNT          474 a          569 c          619 g          480 t
ORIGIN      
        1 ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag tgcgtcgggg
       61 ctggcgacca gcggtaaggg tattcaggtg gttgaagcca ttaacggtgc caccacggag
      121 gaaggggcct ttgtccaggg gaacaggctg caggccggtg cctttaacta ctccctcaac
      181 cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc agaagtcccc
      241 ctgtatgcct ccatgctgac acaggcaatg gactatgacc ggattgtggc aggctcccgc
      301 agccatcaga ccggtgtaaa tggtgaaaac aacagcgtcc gtctcagcat tcagggcggt
      361 catctcggtc acgataacaa tggcggtatt gcccgtgggg ccacgccgga aagcagcggc
      421 agctatggat tcgtccgtct ggagggtgac ctgatgagaa cagaggttgc cggtatgtct
      481 gtgaccgcgg gggtatatgg tgctgctggc cattcttccg ttgatgttaa ggatgatgac
      541 ggctcccgtg tcggcacggt ccgggatgat gccggcagcc tgggcggata cctgaatctg
      601 atacacaacg cctccggact gtgggctgac attgtggccc tgggaacccg ccacagcatg
      661 aaagcgtcaa cggacaataa cgacttccgc gcccggggtt ggggctggct gggttcactg
      721 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtat
      781 acctggcagg gactttccct ggatgacggt aaggacaacg ccggttatgt gaagttcggg
      841 catggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa cgatatgacc
      901 tttggcgaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca cagtgtgagt
      961 gaattaccgg tgaactggtg ggtacagcct tctgttatcc gcaccttcag ctcccgggga
     1021 gatatgcgtg tggggacttc cactgcaggc agcgggatga cgttctctcc ctcacagaat
     1081 ggcacatcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
     1141 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg gtataacggt
     1201 caggccacac tgaatgtgac cttctgacag aaccatcgcc tctctgtggt cccggtcatc
     1261 atgaccggga cccggaccgg cgcaacggat cttcaacgcc acattcgctg gcattaacaa
     1321 taacatgata ttcatcacgg agtgactatg ttacagatag tcggcgcgct gatcctgctg
     1381 atcgcaggat ttgccattct tcgccttttg ttcagagcat taatcagcac ggcttctgcg
     1441 ctggcagggc tcatattgct gtgtctgttc ggcccggcct tactggctgg ctatatcacc
     1501 gaacgcataa cccggttgtt ccatattcgc tggctggcag gcgtatttct gacgattgcc
     1561 ggaatgatca tcagcttcat gtggggactt gatggtaaac atatcgcgct ggaggctcac
     1621 acctttgact ctgtgaaatt tattctgacc accgctctcg ccggtggtct gctggctgtt
     1681 cccctgcaga tcaaaaacat tcagcagaac gggatcacac cagaagatat cagcaaggaa
     1741 attaacgggt attactgctg tttttatact gcctttttcc ttatggcgtg ttctgcatgc
     1801 gcaccattga tcgcgttaca gtacgatatt tcaccgtcac tgatgtggtg gggcgggttg
     1861 ttgtactggc tggctgcatt agtgacgctg ctatgggcgg ccagccagat ccaggcgctg
     1921 aaaaaactga cctgtgccat cagccagaca ctggaagaac aaccggtgct caacagtaaa
     1981 tcgtggctga ccagtttgca aaacgattac agccttcctg actcactgac ggagcgcatc
     2041 tggctgacgc tcatttctca acggatttcc cggggagagc tgagggaatt tgaactggca
     2101 gacggaaact ggttactgaa caatgcctgg tatgaaagaa ac
//
LOCUS       DAYPHN010000299         2089 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000299 DAYPHN010000000
VERSION     DAYPHN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.304"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            378..1322
                     /locus_tag="ACG0BW_004787"
     CDS             378..1322
                     /locus_tag="ACG0BW_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261627.1"
                     /translation="MSPDNISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1403..1825
                     /locus_tag="ACG0BW_004788"
     CDS             1403..1825
                     /locus_tag="ACG0BW_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261628.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEISSDT
                     VIFSILSSLIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
BASE COUNT          670 a          413 c          345 g          661 t
ORIGIN      
        1 tgcaacacaa ttattacaat gaaaaggtat agataaaaaa ccagtgtttt atttaacaga
       61 accgccttat ccacttttta taataccaat tgattagatt agccaatttg ttgctatttt
      121 acataacata tttttatcaa agttaattga ttgttgcgtt tatgagcacc ggccgtaagc
      181 ccgttatgtt attgtttcat tagtaataat cacttaaatg tatcgatgaa acaggtatga
      241 cttagcggga gtgattgttt ctgagtgaac agagaccact ttcgcctgat aaatgaaaaa
      301 tgagcgattt ttgcaagtta gtttttctat agccgagtac gctcagcgcc tcgcttgatt
      361 acaatgaggt tgctgctatg agtcctgata atatcagttc tgctgtcaat cgcttctcag
      421 gttggccatc taatgatcct ctaaaaacag ataaagaatc aatgacatca cccatgactg
      481 tgcaggtaat aaagatgatt gccgccatcc ttgacccaca tcggatagtc gttggaatcg
      541 atacacttta tacaacgcag cgcgaaacat ttacctcaga gagtcaacag ctcattgaat
      601 cagcattgca aacacttcat gtgatgaatt taaataacct ggaaacaaat ctcgatgagg
      661 aaggtcacta tctggctttt ggatataacg ttgacactcc tgacagaata accgtttctg
      721 cttataatga aaaaaagcct ggctgctatg gccaatgtct tttaatacag gatgacgaaa
      781 cagacttatt taacctgata aaagttaaaa tttttgaagc caggcataat tatgtcgctc
      841 aattgatgag cgatccggaa tttatgttca aatttagtta tagcgctcaa caaaatagat
      901 tagaacctca tcttctggtt cccacttatt tccctctaaa aacaaacgaa ccggttacgc
      961 aagaggatgt actgttactt tatcgtttct ttaaaatgga tggtgatttt aatatattaa
     1021 catcagatga atacatgagt atcatgacac ctctaatgaa gtgtgagcgt agcatccacg
     1081 accacaacag gtatgtgacg ggcaaagata cccttcttct ggactatcct ccttctggta
     1141 accagatata tttccatatt tttcctgacg aatcagcaac actagtcctt tatttttcaa
     1201 acaatgacat tgaatgtttc gtctttgaaa gagatattcc gagccagtat cgctttttca
     1261 aaatgtttac caatctggca ctggttattg atcagttaac cgatgccaat caaatacttt
     1321 aatttttata tattccctgt ggcaaatatt gctaaacaat gcccggggat taatttttat
     1381 tactcacgga aagatttaaa taatgatccc atctcaaatc tctttcaaca cccttcctgt
     1441 caatgcaaca tacgcttcag agacaacagt tgacacacaa aaattctctg acatcctcta
     1501 ctcagcgggg tgttccctaa aggatctggc ttatttgagc agatgtctgg catcaataca
     1561 tccgactatc gccaaaaaca tttatgaaac agaaaatctc agcgatcaaa aattactaca
     1621 tattgattgc cgtagtacaa atgaaatcaa gataaatatc ttttttggtc aacaacgaga
     1681 aggattaatt gaaattagtt ctgacacagt cattttttcg atactgtctt cattgattga
     1741 ctccaccatt tctaaatttc aacaccaaaa ccccgttaac ggttctgtaa atcctgacct
     1801 attgtatgag gattatactg attaaatccc tcctgtcgcg aaaaatggcc tgcgttattg
     1861 ccagacgtaa taaaactttt gattaacgtt tctcgtactg gtagcacctt catgtacttt
     1921 tcttttttac gaaaaatcaa aagagctaac aaaggtcata attcgcatga atcattaatt
     1981 atttgttatt ttcaacatta tttttacatt aagcagagat gaattgcagt gaaattcgac
     2041 ttaactttta tgaaatgcat ttactcatgg ctaaagccac gtcacaaaa
//
LOCUS       DAYPHN010000300         2074 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000300 DAYPHN010000000
VERSION     DAYPHN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.305"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(46..972)
                     /locus_tag="ACG0BW_004789"
     CDS             complement(46..972)
                     /locus_tag="ACG0BW_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7261629.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVAGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1129..2049
                     /locus_tag="ACG0BW_004790"
     CDS             1129..2049
                     /locus_tag="ACG0BW_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFK7261630.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          609 a          473 c          436 g          556 t
ORIGIN      
        1 ctcttaccga tattcgccca gagcaaccat caattacaca tatcatcaat tttcgagttt
       61 tactcttttc tccaccataa aatcaataaa agcacgaact ttagagggca ttctcacccg
      121 tgatgaaaca taaatataaa atccaggaaa tgaaacagcc caatcctcaa gaatcggctg
      181 taacttgccc gcattgatat gttcagcgat tgcaaaatcc agatgttgaa tgatacccgt
      241 tccattcagc gccattcgta tcatgctttc gtcatcatta gaaatatact tacctgaaac
      301 ctctatttcg aactcttcat taccccccgc aacattagaa aactcccatg cagagagtgc
      361 accactactt ttatgccgca accgaaggca attatggtta ctcagttcct gaggcgtttc
      421 aggaataccg tgctctttta aataatctgg cgatgccaca gtaaccagtt taataagcgg
      481 agaaacagga atggcagtca tatgctcatc cagtccttgc tcaagacgta tgcccacatc
      541 acagccgtcc gcaataatgt tagataaacc gtcatccata atgagttcta tctttgtttt
      601 ggggtaacga gtaagaaact cgccaatatg aggctcaaca agcatccgtg ctgcaacacg
      661 ggaggaattt attctcaaca ggcctgtagg ttcaagttgg gtatcaccaa catctttcaa
      721 agcatcatca atttgcccca aagccgacac cagcacttca tacagacgct gtccttcctc
      781 agtaagcgac atattgcggg tcgtacggta tatcagcttg gtgttaagac gttcctcaag
      841 agattttaaa ttctgggaca atgcggcccg cgtaataccc atttcagaag cggctctgga
      901 aaaactgccg gcacgaacaa tcagcgcaaa ccaggcaaga gaaggcaaaa ttgaaggctc
      961 tattttcatc attgtaaacc ttaacttaac actgtgatag atattagacc attgatgata
     1021 accaatacaa gtcatactat tacgccgttc tcgacatgaa cacgaatgcc agttgcggat
     1081 aatagaaatg atatttaaat ctgtttaggc aattccccgg agcttgaaat gaaaactgtt
     1141 tcgataaaac atacttactg ggatatagct gctgatatct atttccctga cgattttaat
     1201 agtgaaaaaa agtatcctgc cattatcagc gcccatccaa ttggaagctg caaagaacaa
     1261 acatcgggta gtgtgtatgg tgccgccctg gcaaaagctg gctttatcgt cattgctttt
     1321 gatgccagtt ttcagggaag cagcggtgga gaaccgcgtt atctggaaga tccaacaatg
     1381 cgagtaaaag acttcagcat tgtcgttgat taccttacca ccttacctta cgttgatgcc
     1441 gggcgtattg gtgtactggg tatttgtggt ggaggtggat atgccattaa tgccgcaatg
     1501 acagaacgtc gaattaaagc tattggtact gtcacgggtg caaactacgg gcgtcttatg
     1561 cgtgagggat ttacagcgtt taatcctatc ggagcactcg aagcaatggc gcaacaaaga
     1621 acagacgaag ctaacggcgc aaaacttcgc gtagatgatt tactcccctc ttctccggat
     1681 gcagctcttg aagcgggatt aacagaaata gacctttttg aagccacaga atattatcgt
     1741 tcgccacgag gctgtgcacc taatggggta aatcgttcgc tattctcaca tcagactgtt
     1801 gccgtgggtt gggatgcatt tcatcttgca gaagtcctgt taactcaacc aataatggtt
     1861 gtggtgggcg atagagtggg cgcatttggt gcctaccgtg acggctgcga aattattggc
     1921 cgcgctgcct caaaacataa agagctggtc gtggttgaag gttattcaca ttacgacctg
     1981 tatgataagc cagaacctgt gaaacaggca ctcgataaat taattccttt ttataaaaca
     2041 catttgtaat aatcttacag ggagattcct tatc
//
LOCUS       DAYPHN010000301         2042 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000301 DAYPHN010000000
VERSION     DAYPHN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.307"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..342
                     /locus_tag="ACG0BW_004791"
     CDS             <1..342
                     /locus_tag="ACG0BW_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFK7261631.1"
                     /translation="TLHYVWAREFGECKGKKHYHLMLLVNRDTWCRAGDYRAPESLAG
                     MIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEYTKAHC
                     TGERNFGCSRS"
     gene            602..1003
                     /locus_tag="ACG0BW_004792"
     CDS             602..1003
                     /locus_tag="ACG0BW_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFK7261632.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAEPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            991..1400
                     /locus_tag="ACG0BW_004793"
                     /pseudo
     CDS             991..1400
                     /locus_tag="ACG0BW_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            1684..>2042
                     /locus_tag="ACG0BW_004794"
     CDS             1684..>2042
                     /locus_tag="ACG0BW_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFK7261633.1"
                     /translation="MGNAMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKTAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGQTLWLVFSVRHWYCRNPACSRKIFAESLAPFAGS
                     HQQSSQALQNLQRQLGL"
BASE COUNT          490 a          507 c          558 g          487 t
ORIGIN      
        1 accctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa acactatcac
       61 ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gtgattaccg cgctccggag
      121 tctctggccg ggatgattaa acaggcgtgg tgcagtgccc tgggagtgga tgtcgggtgc
      181 catgccacgc tggtgcattt tccggcctgg ccggcggtgt ggctggcgcg taatgatgac
      241 accggctttc agcaagtgct ggaacgtgct gactatctgg cgaaggagta taccaaagct
      301 cactgcaccg gtgagcgcaa ttttggctgt agccggagtt gagcacgact ggcgcgccaa
      361 tatccggcaa accaaaagta tgggttgttg tctggcggca acgatgtatc cactcgccca
      421 ctgactgttt tttacgtcag tgttccattg tgtgacaacc acagtgctgt aacagcaacc
      481 agcctgcttt acccaaactc cagtccagat tttaactgaa ccgccattca caccctgacc
      541 acgctgcccg taccgggacg gttgttgcct gcgtgtcgtc tggcggctaa ggaatatgac
      601 tatgtacgca aaatccttta tcgctcttga tggcaacgga cgtctgacgg gcgcccgtac
      661 tgcacaggcc gaaccttatg ctcactacac ctgccacttg tgtggcagtg cactcagata
      721 ccatccgcaa tacgacactg aacttccctg gtttgaacac actgacgaca ggctgacaga
      781 gcacggtcaa cagtgccctt atgtcaggcc ggagcgcaga gaaatacagt tgattaaacg
      841 tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt aaagccagct ggcactgcag
      901 acaatgtcac cacgattatt atggggagca gtactgcaca cactgccaga ccggaggatt
      961 cagtattccc cggacaactc aggaggaaat atgcgaattc tgaactgtta tatggcgaat
     1021 gacagcaaag gccattttgt tacggcgaaa gaagctgcga agcacaaccg acaggacgtt
     1081 ttgtgctgtg tgtcctgtgg atgcccgtta acacttcagc ggggcaatga cggacaacca
     1141 ccgtggtttg aacatgacca gatgactgtc gctgagaaaa tcctgctgcg atgcacctgg
     1201 cttgacccgg cagagaaaga ggcccgtcgt ttgcatctgc agggtatgac ggttccggat
     1261 tatacggtga aggtgagaaa gtggttttgt gtgatgtgtg acgaagatta tgaaggagaa
     1321 aagtgctgcc cgcgttgtgg taccggggta tacagcagtg aggggggctg caggaaggca
     1381 actggaagga taggaactga gcggttcagt cgcggaaatc agttgcagag ctgtaatgat
     1441 atctccggga gtgctgcgtt ggtaggggca tcccgggata gagaaatttt agcactttgc
     1501 cctgaatgat tgtagacgtt ccgagtactc gtaccggtct gagttattac agttagatat
     1561 tcactttttc tcccgcatac ttcacatctg tcaacgacgg atgaaaagtg gttcttccgc
     1621 aacctgggtg gaagaaaatg gatgaaactt tctggcgaac aaggatctaa gctgttttaa
     1681 gttatgggca acgcaatgca ctctcttaag acacttctac agttaccttg cggatggcga
     1741 tgcagtcgac aaattattag ctctgacggt ataaccctcc atctccacgg aaaacgcaaa
     1801 acagcacaat gtcctgaatg ctttaagcgt agcgactctg ttcatagttg tcgtcggcgc
     1861 cggatacagc atctaccctg ctcagggcag acgctatggc ttgtattttc cgtccgccac
     1921 tggtactgcc gtaacccggc ttgttcacgt aaaatttttg ccgagtcgct tgctcccttc
     1981 gccggttcac accagcagtc ttcacaggcg ttacaaaatt tacaacgtca actgggatta
     2041 at
//
LOCUS       DAYPHN010000302         2041 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000302 DAYPHN010000000
VERSION     DAYPHN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.308"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            123..995
                     /locus_tag="ACG0BW_004795"
     CDS             123..995
                     /locus_tag="ACG0BW_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFK7261634.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVKRFRLSGHGHSMVITDLPGVGESRERDA
                     EYEALYRDILPELDLVLWLIKTDDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCIASVACTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1368..>2041
                     /locus_tag="ACG0BW_004796"
     CDS             1368..>2041
                     /locus_tag="ACG0BW_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFK7261635.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVA"
BASE COUNT          477 a          530 c          576 g          458 t
ORIGIN      
        1 ttcatgttaa tacgcaacaa tcccatcgat tcaaaacaat ttcagacaga tattatctcc
       61 gtgaatcccc cgccaccttt ccggtgcgcg gggttttgtc ttttttcacc gggaatacat
      121 gtatgaatcc gtctgatgcc attgaggcaa ttgaaaaacc actttcttcc ctgccttact
      181 cgctttcccg tcacatcctg gaacatctgc gcaaactcac cagtcacgaa cccgtgattg
      241 gcattatggg taaaagcggg gccggtaaat cctcactctg taatgcgctg tttcaggggg
      301 aggtcacacc ggtcagtgat gttcacgccg gcacccggga agtgaagcgt ttccgtctga
      361 gtggacacgg tcacagcatg gttatcactg acctgcccgg ggtgggcgag agccgggagc
      421 gggatgcaga gtatgaagct ctgtaccgtg acattctgcc cgaactggac ctggtactgt
      481 ggctgattaa aaccgatgac cgtgccctgt ctgtggatga gtatttctgg cgacacatcc
      541 tgcaccgggg acatcagcag gtgctgtttg tggtgacgca ggccgataaa acggagccct
      601 gccatgaatg ggatatggcc ggtattcagc cttctcccgc acaggcacag aacattcgcg
      661 aaaaaacgga ggcggtattc cgtctgttcc ggcccgtaca tccggttgtg gccgtatcgg
      721 cccgcaccgg ctgggaactg gatacgctgg tcagtgcgct catgacagcg cttcccgacc
      781 atgccgccag tcccctgatg acccgcctgc aggacgagct gcgcacggaa tctgtccgcg
      841 ctcaggcccg tgaacagttt accggtgcgg tggaccggat atttgacacg gcggagagcg
      901 tctgtattgc ctctgttgca tgcacggttc tgcgtgccgt ccgtgacacg gtggtctctg
      961 ttgcccgcgc tgtatggaac tggattttct tctgaacact ctgccagggg aaatgcctga
     1021 ttacattgcg ttaaccaact gaaaacatga cacaaacacc tcatacccct cctgtttctc
     1081 ttgcgtggcc gccttgttat taatagaata aaacgatcga taaaacaggt attgatcgtt
     1141 actatcgatc gtttatagtg gttaatggtc tgcgaaaagg cagctgaatc tcttcatcat
     1201 gcagaacgga attgcacaca acggactgat acttctctgg cggatgagag aggggaagca
     1261 tttatgtccg ggaaaaacca ctttatctga gcatgctgtt ttgtacctgc cggtatccac
     1321 atttgtgggt accggctttt ttattcaccc tctgtaagga aaagctgatg aaacgacatc
     1381 tgaacaccag ctacaggctg gtatggaatc acattacggg caccctggtg gtggcctccg
     1441 aactggcccg ctcacgggga aaacgcgctg gtgtggcggt tgcgctgtct cttgctgctg
     1501 tcacatcagt cccggcactg gctgctgaca aggttgtaca ggcgggagaa accgtgaacg
     1561 acggaacact gacaaatcat gacaaccaga ttgtcctcgg tacggccaac ggaatgacca
     1621 tcagtaccgg tctggagtat gggccggata acgaggccaa taccggcgga caatggatac
     1681 aaaatggcgg tatcgccaac aacactactg tcaccggtgg tggtcttcag agagtgaatg
     1741 ccggaggaag cgtttcagac acggttatca gtgccggagg cggacagagc cttcaggggc
     1801 aggcagtgaa caccactctg aacggcggtg agcagtgggt acatgaaggc gggattgcaa
     1861 cgggtaccgt cattaatgag aagggctggc aggccatcaa atccggcgca gtggcaaccg
     1921 acacggttgt gaataccggc gcggaagggg gaccggatgc ggaaaatggt gataccgggc
     1981 agaccgtcta cggagatgcc gtacgcacca ccatcaataa aaatggtcgt cagattgtgg
     2041 c
//
LOCUS       DAYPHN010000303         2013 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000303 DAYPHN010000000
VERSION     DAYPHN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.310"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            374..1249
                     /locus_tag="ACG0BW_004797"
     CDS             374..1249
                     /locus_tag="ACG0BW_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFK7261636.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATAQHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQILALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1257..>2013
                     /locus_tag="ACG0BW_004798"
     CDS             1257..>2013
                     /locus_tag="ACG0BW_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFK7261637.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPI"
BASE COUNT          569 a          476 c          460 g          508 t
ORIGIN      
        1 ttaacgtacg ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc
       61 gtattccccc cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
      121 gcaggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
      181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac ccataatatc
      241 ggctggtaaa catcactcgc tagtgcattc tttttgttgg taatgataat catttacatt
      301 cattctttga tgttttatca taattaatgt tttgtcaata aatatatgga aacatattgt
      361 ttcagatggg aatatgagaa agttcatcct tatttctatg ataacgttag ctagtgttag
      421 tctgggagcc tgcagacaga atgtcaccat aaagcaggat gcgccagggc agaaagcttt
      481 cctgactgac gggcagattt acgatttgca cagtggcaaa attatttcta gcagcgagct
      541 tttggcggat cttgctaccg ctcaacatct tattattggt gaaaaacacg ataatgccga
      601 gcatcatcag attgagcttt ggcttattca aaatttgctc atccagcgac cacaggggag
      661 tgtgttgctt gagatgttga cgtcagagca acagccccga gttaaccaag ttaaatgttg
      721 gctgaaagat aatcctgtgg ttcgagatag ccgtgttcag gaactactta actggcaaaa
      781 agggtggtcc tgggagatgt atggcgacat cgtcatgcag ctattacgtg ggccttaccc
      841 gctgctcaat gccaacattg gccgggagca gatattggca ctgtacaaaa aaaacgaatt
      901 tcctaaagga aaaaaatcca ctgcacctgt ggtacaagaa gctctgcgtg aaaccatcat
      961 atctatgcat gaaggcaacc ttgaaagcca gcaactgacg tcaatgctct ctattcagca
     1021 gcagcgggat cgctacatgg cccgccagct tctgagtgcc ccggtaccgt cgttgttgat
     1081 tgcaggtggt tatcacgcgt caaaaagcat gggcgtaccg ttacacatgg aagatcttgc
     1141 caccggcacc catccagtgg tgttgatgct tgcagaaaaa ggaatgaaca tcacagtaga
     1201 tcatgctgac tacgtgtggt ttgtagcgcc agatacaaca aaaaggtaaa ccatggatga
     1261 tgaatattct ccacttctca cagtcggtaa aatggagcag ctggtttatc tgttctctac
     1321 tgcttcatgg actgattttc cttgcgttta tctggcgttt ttccgaagtt cagcccgcta
     1381 tgtctcctgc accggcgatc atgctgcaat gggcagaaat tatagaggct ccttcttctc
     1441 cactttcgct tccggtggga atcgctcaac aggagtccgc agtgacagaa gaaaagcaac
     1501 aaactgagga cagacaacaa cgtcctgtca ctgaagacag tgatgcaacc attgaaataa
     1561 ctaggaagaa aaagtcatcc gatggagaaa agaaaaagac acgtccacct cgtaagataa
     1621 aggctcaaac cagcgacagc aaccccacag ccgtttccag taatgctgca cctcaggctc
     1681 tggtcgaatc gtcccgtatt gctgcgcctt ttaacagtga ctcaacaaag cgagataatt
     1741 ctgaggcatc ttgggaaagc agagtaaaag gccacctaaa ccgttataaa cgttaccctg
     1801 gcgatgccag aaaacgtgca cgaaccggca cagccgtcgt gacttttacc gtaaacacgg
     1861 aaggcactat tgtcagtagt ttcttggaaa tctcttcagg aaccttttct cttgaccgcg
     1921 aagctattgc tgttttagaa cgcgctcaac cacttcccaa accaccgcct gaaatattgg
     1981 agggaggatt atttaaagta aaaatgccga tta
//
LOCUS       DAYPHN010000304         1962 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000304 DAYPHN010000000
VERSION     DAYPHN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.312"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            16..294
                     /locus_tag="ACG0BW_004799"
     CDS             16..294
                     /locus_tag="ACG0BW_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261638.1"
                     /translation="MNIDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLSGRD
                     VIILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
     gene            375..461
                     /locus_tag="ACG0BW_004800"
     CDS             375..461
                     /locus_tag="ACG0BW_004800"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFK7261639.1"
                     /translation="MSVLADGVLVAGVVVIAFPLLKKKTPDC"
     gene            complement(555..686)
                     /locus_tag="ACG0BW_004801"
                     /pseudo
     CDS             complement(555..686)
                     /locus_tag="ACG0BW_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            996..1256
                     /gene="tolA"
                     /locus_tag="ACG0BW_004802"
                     /pseudo
     CDS             996..1256
                     /gene="tolA"
                     /locus_tag="ACG0BW_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
     gene            1428..1802
                     /locus_tag="ACG0BW_004803"
     CDS             1428..1802
                     /locus_tag="ACG0BW_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFK7261640.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
BASE COUNT          548 a          352 c          488 g          574 t
ORIGIN      
        1 tatggtgtgg gtagagtgaa tattgatgag gcgtcggggt gtttcatcct taggcagcgt
       61 attgatatag tcaatgcagc acgagcaaag gccttcagcc gtttgacagt tttgttttgt
      121 actcctgatc gtctttcggg aagagacgtt attattctga atagtgatgc tatacagagg
      181 gtttgcgatg agttcatggt tgctaattca gaattatttg ctcttgttca ggagtacaac
      241 agaatagcca ggacctgtgg tatggatgaa cttcggatta ctcatctggg gtagatacat
      301 atctggatta tcacctgtta cggtaaaaag ttattgctta ctgtttttgt gaatggcatt
      361 gcagcagccg gataatgtca gtgctggctg acggtgtgct ggtggcgggt gtggtggtta
      421 ttgctttccc gttgctgaaa aagaaaacgc cagactgtta gccgggtatc agttagcggg
      481 agaaattttt aaatacttca caattcaggc ggttgactgt tgtctggttt gcggggagtt
      541 tgttaaaaga aactggcatg gtgaatcccc ctgtgcggag gggcaatcag cgagtaggta
      601 tatgggataa tcgcgtattc aggtgctggt actgaattca ccgggaggca cccggcacca
      661 tgcacttcaa tagattctct ccacattatg gatattcttt cagaatatcc cacgcagact
      721 ttgtgtaaat gttaacaaat gtgcgtttta tttgatctga ttcgctgttt gagcgtccaa
      781 aacaacggta tatataatcc tttactatat gacatatgtg aggaaaaatg ggttttcgta
      841 gcgcatcaat tcttacgttg attattagtg ggattattat cgggtgcact gatgctgtat
      901 cgacaaatta tcatgaccgt acatcatatt actccgataa agcaatagag acacagtatg
      961 tgagttcatc tgaacgtact tctgatgtta gtgaggatat ccgtctgtat gcccatcaaa
     1021 tcaagagcgc catcgaaaaa cagttcgggg atgcgagtaa gtattcagga aaagagtgta
     1081 cactgagaat gcatatggcc ccgaatggcc ttctactgga ggttaaaaga gaaagtggag
     1141 acctcgattt atgtcgtgaa gcgatgaatg cgataaagaa tgctgatata cctgcccccc
     1201 cttcgccgga agtatataaa gtatttcaaa atggggtgct ggattttaaa ccctgatatt
     1261 tattgttttg taataaacgg tttcggctta ggtttgttct gacacaacta cggcactgag
     1321 ctaaatttag cggatagtca gctctgagcc agtggcggac gtaacaacta ctattgctga
     1381 gattttaatg gattgaggag caagaagtgg gattaaagaa aatcgttatg ttgacttttt
     1441 gggtcggttt tgttgcggga tgcacacctt tacacccttc agattgccac aaaactactg
     1501 ctacaggtag ttgcagttca ggacgctggg atgatcagga tgaatggggg gcgcaagcgc
     1561 ggggaatcag agctgcaatt aatgccaaac ttgatgagcc gcataactgg aaagggaaaa
     1621 aatgcaggtt gcatatggaa ttctctcagg atggcacggc gttaaaaata tctaccagta
     1681 acggtgataa agcctattgc gaagcgataa agtccgcagc tcataaagcc aaatttccgg
     1741 ccttcaacaa tccggaagtc tacagagatt ttcagaaatc tggctttgac atgcgaggtt
     1801 agctcttcaa ttactatatc tcattcatag caaactgaca gatttgatga tgttctatat
     1861 acgaaacctg tgatgtcaag tctgagctaa tacaaataaa cataatatca gagaaataca
     1921 ttttattagc tcgctacggc gagcttttta tattgcatcg tc
//
LOCUS       DAYPHN010000305         1899 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000305 DAYPHN010000000
VERSION     DAYPHN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.313"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            244..1044
                     /gene="ydcF"
                     /locus_tag="ACG0BW_004804"
     CDS             244..1044
                     /gene="ydcF"
                     /locus_tag="ACG0BW_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFK7261641.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1241..>1899
                     /locus_tag="ACG0BW_004805"
     CDS             1241..>1899
                     /locus_tag="ACG0BW_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFK7261642.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERAGEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NP"
BASE COUNT          488 a          454 c          458 g          499 t
ORIGIN      
        1 attgattgag aaatataaga atgaatattt cataaatatc acaccaactt atctgtcact
       61 taaaaatgat agaacattat gttattgatg ttttatcaac agtttctccg caaatacagt
      121 atgactaaaa ccagtgtctg gttcgcgatg gcgcgataga aaaaggatca tcttcctttc
      181 agagtgctcc gcttctcact attatctcac gcagtattct taaaggaacg ataaggagga
      241 accatgaaca ttaccccgtt tccgacgctt tcgccggcaa ccatagatgc tataaatgtt
      301 atcggacagt ggctggcgca ggatgatttc tccggtgagg tgccgtatca ggccgattgc
      361 gtgatccttg caggcaatgc ggttatgccg actatcgacg cggcatgtaa gattgctcgc
      421 gatcagcaaa ttcctttact gattagtggt ggtatcggtc actcgacaac ttttttgtat
      481 agcgccatcg cacagcatcc gcactacaac actatccgca ccactggcag agcagaagcg
      541 accatcctgg cggatatcgc tcatcagttc tggcatattc cgcatgaaaa aatctggatt
      601 gaagaccagt caacaaactg tggtgaaaac gcacgcttta gcatcgcgct attgaatcag
      661 gccgtagaac gagttcacac agctatcgtt gttcaggacc ccaccatgca gcggcgcacg
      721 atggcgacgt tccgccgtat gaccggggac aatcccgatg caccacgctg gttgagttat
      781 cccggattcg ttcctcagtt aggaaataac gcagacagtg taatctttat taatcagtta
      841 caaggattat ggccggttga gcgttatcta tcactactca ctggcgagct gccgcgttta
      901 cacgatgata gcgatggcta cggtccccgc gggcgagatt ttattgttca cgttgatttt
      961 ccggcagaag tcatccaggc atggcaaacg ctgaaacacg atgcggtgct catcgaggcg
     1021 atggaaagtc gctcgttacg ttaaaaattg cccgtttgtg aaccacttgt ttgcaaacgg
     1081 gcatgactcc tgacgtttat ttctgccttt tattcctttt acacttgttt ttatgaagcc
     1141 cttcacagaa ttgtcctttc acgatgccgt ctctatgatg attgatgtta attaacaatg
     1201 catttaccaa aaacaaacat ataaatcaca ggagtcgccc atgtcagtac ccgttcaaca
     1261 tcctatgtat atcgatggac agtttgttac ctggcgtgga gacgcatgga ttgatgtggt
     1321 aaaccctgct acagaggcgg tcatttcccg catacccgat ggtcaggccg aggatgcccg
     1381 taaggcaatc gatgcagcag aacgtgcaca accagaatgg gaagcgttgc ctgccattga
     1441 acgcgccagt tggttgcgca aaatctccgc cgggatccgc gaacgcgccg gtgaaatcag
     1501 tgcgctgatt gttgaagaag ggggcaagat ccagcagctg gctgaagttg aagtggcttt
     1561 tactgcggac tatatcgatt acatggcgga gtgggcacgg cgttatgaag gtgagattat
     1621 tcaaagcgat cgtccaggag aaaatattct tctgtttaaa cgtgcgcttg gcgtgactac
     1681 cggcattctg ccgtggaact tcccgttctt cctcattgcc cgcaaaatgg ctcccgctct
     1741 tttgaccggt aataccatcg tcattaaacc cagtgaattt acaccaaaca atgcgattgc
     1801 ttttgccaaa attgtcgatg aaataggcct tccacgcggt gtgtttaacc ttgtgctggg
     1861 gcgtggtgaa accgttgggc aagaactggc gggtaaccc
//
LOCUS       DAYPHN010000306         1873 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000306 DAYPHN010000000
VERSION     DAYPHN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.314"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            124..1500
                     /gene="ygeH"
                     /locus_tag="ACG0BW_004806"
     CDS             124..1500
                     /gene="ygeH"
                     /locus_tag="ACG0BW_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFK7261643.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQIYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKRNEDNTSDYSIAIFPFTTSVNTLDPLILNQELVQNISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFKPDYFVTGRINQNNAVKTLYIELIDAKNLFLIASNHLPVDE
                     LNNTSQFIIDNILQTVHNPERSVRLAKSDQGYKNHYLSDELLAGKKELYEFTSESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKYELELAAQKALELLHYVSDIT
                     NVDGKILAIMGLITGLSGQAKVSHILFEQAKIHTTDIASLYYYRALVNFHNERIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKSNMKLYYKETSSESHRVIIDNILKLK
                     QLTRICMR"
     gene            1668..>1873
                     /locus_tag="ACG0BW_004807"
     CDS             1668..>1873
                     /locus_tag="ACG0BW_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261644.1"
                     /translation="MTNPINMNNFSQSLNIATATGDEVVSLDKHITTSTTDTDQIQAF
                     IVSTWMASFQNDMYSKDNPISPYY"
BASE COUNT          680 a          317 c          315 g          561 t
ORIGIN      
        1 acacaaccat agatatgtcg aatgtaaact cctatagcat taatgacaca taaatatgaa
       61 tagccacaat ttctattgct tatgcaggat gcaagaaacc aattttttca tagaggttaa
      121 ctaatggact tagaaaataa attttcatat cattttcttg agggattaac gctcacggaa
      181 gatggaattc ttactcaagg aaatgagcag atttatattc caccgaaaga gttaggagta
      241 ttgatcgtat tacttgaatc cgctggtcat gttgtcctga aagatatgat catcgaatca
      301 gtatggaaaa atattattgt aagtgacgag tccctgacaa gatgtatcta ttctttgcgc
      361 tgcatttttg aaaaaattgg ctatgatcgt tgcatagaaa caatctaccg gaaaggttat
      421 cgtttcagtg gacaggtttt caaaactaaa agaaatgaag ataacacttc agactattcc
      481 atagctatat ttcctttcac cacttcagtg aataccctgg atccattaat acttaatcag
      541 gaattagtgc aaaacatttc gaataaaaaa atcgatggcc tttataccta tccgatggct
      601 gcgacaaatt tttgtaatga tcacatatcg caaaattcat tcttgagaag attcaagcca
      661 gattatttcg ttacaggaag aataaaccag aataacgcgg tgaaaacttt atacattgaa
      721 ctgatcgacg ctaaaaatct tttcctcatc gccagtaatc atcttcctgt tgatgaacta
      781 aataacacat cacaatttat tatagataat atccttcaaa cggtacataa tccagagcga
      841 tctgtaagat tagctaaatc agatcaaggt tacaagaatc attatttatc agatgaactg
      901 ttggctggta agaaagaact ttacgagttt acatctgaaa gcatttacag ggctatgact
      961 atatttgatg gattacaaaa taaaagtgat atacagacgc taaaaacaga atgttattgt
     1021 ctgctagcgg aatgccatat gtctttggca cttcatggaa aatatgaact tgaacttgct
     1081 gctcaaaaag cattagagct tttacattat gtatcagaca taaccaatgt cgatggaaaa
     1141 attttagcta ttatgggact gataactggt ctgtctggac aagcaaaagt atctcatatc
     1201 ttatttgaac aggctaagat acacacaact gatatagcct ctctctacta ctatagagca
     1261 cttgtcaatt ttcataatga aagaattgaa gaggcaagga tttgtataga caaatcacta
     1321 caactcgaac ccagaagacg aaaagcagtt gtgataaaag agtgtgtaga tatgtatgtg
     1381 cctaacccgc tcaaaagcaa catgaaactc tactataaag aaacttcgag tgaaagccat
     1441 cgagttataa ttgacaacat tttgaaacta aagcaactga cgagaatttg tatgcgataa
     1501 atctttattg tcgcaatttt attgtttaga tgcatgcaac tggttatgta aacaacagag
     1561 atgaaaacat ctaagataat taccttattg caatatatat caaatactct agattaaaaa
     1621 gtattgtgta taggctattg gcctggacaa ataaaaaagg gaactttatg actaatccaa
     1681 tcaatatgaa taatttttct caaagtttaa atatagcaac cgcaacgggt gatgaagtcg
     1741 tttcattaga taaacacatc accaccagta ctactgatac agatcaaata caagcattta
     1801 tagtttcaac atggatggca tcttttcaaa atgatatgta ttcgaaagat aatcctatct
     1861 ctccttacta tga
//
LOCUS       DAYPHN010000307         1849 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000307 DAYPHN010000000
VERSION     DAYPHN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.315"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            144..1019
                     /locus_tag="ACG0BW_004808"
     CDS             144..1019
                     /locus_tag="ACG0BW_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-27"
                     /protein_id="HFK7261645.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1054..1416
                     /locus_tag="ACG0BW_004809"
                     /pseudo
     CDS             1054..1416
                     /locus_tag="ACG0BW_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1476..>1849
                     /locus_tag="ACG0BW_004810"
     CDS             1476..>1849
                     /locus_tag="ACG0BW_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFK7261646.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT          433 a          478 c          530 g          408 t
ORIGIN      
        1 aaaggtggtt gtaaataatg ttacaatgtg tgagaagcag tctaaattct tcgtgaaata
       61 gtgatttttg aagctaataa aaaacacacg tggaatttag ggaatactga tgtaacacgg
      121 attgaccgta ttgggagttt gagatggtga caaagagagt gcaacggatg atgttcgcgg
      181 cggcggcgtg cattccgctg ctgctgggca gcgcgccgct ttatgcgcag acgagtgcgg
      241 tgcagcaaaa gctggcggcg ctggagaaaa gcagcggagg gcggctgggc gtcgcgctca
      301 tcgataccgc agataatacg caggtgcttt atcgcggtga tgaacgcttt ccaatgtgca
      361 gtaccagtaa agttatggcg gccgcggcgg tgcttaagca gagtgaaacg caaaagcagc
      421 tgcttaatca gcctgtcgag atcaagcctg ccgatctggt taactacaat ccgattgccg
      481 aaaaacacgt caacggcaca atgacgctgg cagaactgag cgcggccgcg ttgcagtaca
      541 gcgacaatac cgccatgaac aaattgattg cccagctcgg tggcccggga ggcgtgacgg
      601 cttttgcccg cgcgatcggc gatgagacgt ttcgtctgga tcgcactgaa cctacgctga
      661 ataccgccat tcccggcgac ccgagagaca ccaccacgcc gcgggcgatg gcgcagacgt
      721 tgcgtcagct tacgctgggt catgcgctgg gcgaaaccca gcgggcgcag ttggtgacgt
      781 ggctcaaagg caatacgacc ggcgcagcca gcattcgggc cggcttaccg acgtcgtgga
      841 ctgtgggtga taagaccggc agcggcggct acggcaccac caatgatatt gcggtgatct
      901 ggccgcaggg tcgtgcgccg ctggttctgg tgacctattt tacccagccg caacagaacg
      961 cagagagccg ccgcgatgtg ctggcttcag cggcgagaat catcgccgaa gggctgtaac
     1021 tggttttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gtatcttccc
     1081 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gcccacctac
     1141 aataaagccc tcatcaaccg tggctccata actttctggc tggatgatga agctattcag
     1201 gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgcta ttctgacctt
     1261 gccatcacga ctgtgctggt cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag
     1321 ggctttattg attccatttt ttctctgatg aacgttccgc tacgctgccc ggattacagc
     1381 tgtgtcagca ggcgggcaaa gtcggttaat atggcactgt tgcaaatagt cggtggtgat
     1441 aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa ggccggcatt
     1501 ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc atcagttacc
     1561 gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc acgatttacc
     1621 gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac tggcgtaacc
     1681 cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat ggccgctggg
     1741 cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat ctctcctccc
     1801 gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaa
//
LOCUS       DAYPHN010000308         1846 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000308 DAYPHN010000000
VERSION     DAYPHN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.316"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            156..659
                     /gene="ftnB"
                     /locus_tag="ACG0BW_004811"
     CDS             156..659
                     /gene="ftnB"
                     /locus_tag="ACG0BW_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFK7261647.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(739..990)
                     /gene="yecJ"
                     /locus_tag="ACG0BW_004812"
     CDS             complement(739..990)
                     /gene="yecJ"
                     /locus_tag="ACG0BW_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFK7261648.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="ACG0BW_004813"
     CDS             complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="ACG0BW_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFK7261649.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1454..1777
                     /gene="yecR"
                     /locus_tag="ACG0BW_004814"
     CDS             1454..1777
                     /gene="yecR"
                     /locus_tag="ACG0BW_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFK7261650.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          472 a          435 c          414 g          525 t
ORIGIN      
        1 tacccgttca gacgttattc ttatttcaga tcatcgtcag aattgactcc acgatcacat
       61 ttcggaccgg cagaaaggaa ttattctgca aacagtaatt atggtgtttt gatttatctt
      121 gcacctctcc acttctggat ataaggatat taggtatggc aaccgctgga atgcttctca
      181 aactcaactc tcaaatgaac cgcgagtttt acgcatccaa tctctacctt cacctgagta
      241 actggtgttc tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc caggcacaga
      301 gtaatgtgac ccaaatgatg cgcatgttta actttatgaa gagtgtcggc gctaccccca
      361 tcgttaaagc cattgatgtt cccggtgaaa aactgaactc tctggaagaa ctgttccaaa
      421 aaacgatgga agaatacgag caacgttcaa gcacgctggc gcagttagcc gatgaagcga
      481 aagaactgaa tgatgattca accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc
      541 atgatggcct gttgctgcaa accattcttg atgaagtgcg tagtgcgaaa cttgcgggta
      601 tgtgccctgt gcagaccgat caacatgttc tgaatgtcgt gtcgcatcag ctgcattgat
      661 catacatcgg cgctaatgca ttgcgccgat gaagtttttg agaaaccgct gcctcatctt
      721 tttgaagcag cggttttttt aatgggattc gccctgtggg gtaaacttga gttcaatcag
      781 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttagt
      841 aaagtcgata ttcttcagct ggctggacat tttttcacga acttcaacgg gcgcgattac
      901 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag
      961 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     1021 ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa
     1081 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     1141 cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca tcgcggcact
     1201 ccgaccattt gttatacagg tgttattgtc tttgcgctta taatatgaca atcatcacaa
     1261 aaatcaatct ttatgtgatg caaatcacat aaatacccct ttgatgttat aaaaatgata
     1321 atcaaaaagc agctacccca tttctgacac ctacagatgg caagaaatag cgcctgccag
     1381 gcgtcttttc cggccattgt cgcagcgctg taacgcgtaa aatagtgctt tcttttactc
     1441 ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc tggcgggatg
     1501 tacggttact cgccaggccc atgtgagcga agttgacgcg gcaacaggta ttgtacggtt
     1561 ggtttatgac caggcttttt tgcagcatgc ccataccgat cgttatgtca gtcgcgggat
     1621 cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg gtcagccagt
     1681 aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca ctttgtcata
     1741 tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag cgaggggatc
     1801 ttcgctgatt aaagaaatag cggtaatgcg ttttattccc attcgt
//
LOCUS       DAYPHN010000309         1842 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000309 DAYPHN010000000
VERSION     DAYPHN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.317"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            13..120
                     /locus_tag="ACG0BW_004815"
     CDS             13..120
                     /locus_tag="ACG0BW_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261651.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGKQV"
     gene            326..1609
                     /gene="gltA"
                     /locus_tag="ACG0BW_004816"
     CDS             326..1609
                     /gene="gltA"
                     /locus_tag="ACG0BW_004816"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFK7261652.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          489 a          444 c          424 g          485 t
ORIGIN      
        1 gaaaagcata taatgcgtaa aagttatgaa gtcggtattt cacctaagat taacttatgt
       61 aacagtgtgg aagtattgac caattcattc gggacagtta ttagtggtaa acaagtttaa
      121 taattcggat tgctaagtac ttgattcgcc atttattcgt catcaatgga tcctttacct
      181 gcaagcgccc agagctctgt acccaggttt tcccctcttt cacagagcgg cgagccaaat
      241 aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt taagttccgg cagtcttacg
      301 caataaggcg ctaaggagac cttaaatggc tgatacaaaa gcaaaactca ccctcaacgg
      361 ggacacagct gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg ttattgatat
      421 ccgtactctc ggttcaaaag gtgtgttcac ctttgaccct ggcttcactt caaccgcatc
      481 ctgcgaatct aaaattactt ttattgatgg tgatgaaggt attttgctgc accgcggttt
      541 cccgatcgat cagctggcga ccgattctaa ctacctggaa gtttgttaca tcctgctgaa
      601 tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa actacggtga cccgtcatac
      661 catgatccac gagcagatta cccgtctgtt ccatgctttc cgtcgcgact cgcatccaat
      721 ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc tatcacgact cgctggatgt
      781 taacaatcct cgtcatcgtg aaattgccgc gttccgcctg ctgtcgaaaa tgccgactat
      841 ggccgcgatg tgttacaagt attccattgg tcagccattt gtttacccgc gcaacgatct
      901 ctcctatgcc ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac cgtatgaagt
      961 taatccgatt ctggaacgtg ctatggaccg tattttgatc ctgcacgctg accatgaaca
     1021 gaacgcctcc acctccaccg tgcgtaccgc tggctcttcg ggggcgaacc cgtttgcctg
     1081 tatcgcagca ggtattgctt cactgtgggg acctgcgcac ggtggtgcta acgaagcggc
     1141 gctgaaaatg ctggaagaaa tcagctccgt taaacacatt ccggaatttg ttcgtcgtgc
     1201 gaaagacaaa aatgactctt tccgcctgat gggcttcggg caccgcgtgt acaaaaatta
     1261 cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa gtgctgaaag agctgggtac
     1321 caaagatgac ctgctggaag tggctatgga gctggaaaac atcgcgctga acgacccgta
     1381 ctttatcgag aagaaactct acccgaacgt cgatttctac tctggtatca tcctgaaagc
     1441 gatgggtatt ccgtcttcca tgtttaccgt tatcttcgcg atggcacgta ccgttggctg
     1501 gatcgcccac tggagcgaaa tgcacagtga cggtatgaag attgcccgtc cgcgtcaact
     1561 gtatacagga tatgaaaaac gcgactttaa aagcgacatc aagcgttaat ggttgattgc
     1621 taagccgttt actttccgga cccgccttta atagagcggg ttctttttta tatggctgaa
     1681 cataataaat tgtaaaaatt aaaccagatg caaataccca ttaataaggt gaaaaaatgt
     1741 gacaagtcac atactgagca gaatgataag tttattttta tactttcctg ccggtagcat
     1801 tgttaaccca atgaaaatta tgcatatcca tcccacccca tc
//
LOCUS       DAYPHN010000310         1825 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000310 DAYPHN010000000
VERSION     DAYPHN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.318"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            43..330
                     /locus_tag="ACG0BW_004817"
                     /pseudo
     CDS             43..330
                     /locus_tag="ACG0BW_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            773..>1825
                     /locus_tag="ACG0BW_004818"
     CDS             773..>1825
                     /locus_tag="ACG0BW_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFK7261653.1"
                     /translation="MKFPSIFNKINPQSIQQHPEKNELNWMLELNQWKAERILTGEIH
                     RPECRNEAAKRINCAFLSKQNNIDLSGLNLTTQPPGLQNFTSINLDENQLKHFDATTY
                     DRLINLSLNSNALESINFPQGRNVSITHISMNNNSLRNIDIDRLSSITYFSAAHNQLE
                     FVQLECCERLQYLNLSHNQLTDIVAGNKDELLLLDLSHNKLTSLHNTLFPNLNTLLIN
                     NNLLSEIKIFFSNFCNIQTLNAANNQLEKINLHFLTYLSSIKSLRLDNNKITRIDTKN
                     TSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFE
                     IHSEGVDVDNELFHYTI"
BASE COUNT          660 a          364 c          271 g          530 t
ORIGIN      
        1 tcccggcaag aatctctgat tgagttaagt gcccatcaca atatgatcac tgaccttata
       61 ttacacaatc atcccaggat gaaaaaaatc actttaaacg acaaccatat tgcacactta
      121 aacgcgaaaa ccactacaaa actggaatat ttaaacttaa gcaataacaa tttattgcca
      181 acagatgaca ttgatcaatt aatatcatca aaacatcttt ggcatgtatt agttaacggc
      241 atcaacaatg atccacttgc ccaaatgcag tactggactg cagtaagaaa tataattgat
      301 gacactaatg aagtgaccat tgaattatct tataacctgg caatcacaaa tatcgatacc
      361 agcgatgaac atcttgtaga agtaagcgag aattccgaag gaaatcatat aaaagacaat
      421 gactcaatgt ctattcgtta tagatcaaaa tattattcca gagagtacgc tttaatagaa
      481 gaagaaacaa tattttctga cgcagaacta aaagccattc tgcctatgca tcgcatgtac
      541 ggggttggtg actataagtc aaattcctct tctctaccct cacactcggg gctaaaggac
      601 ccaacgggca cacccgtctg ttattatatt cataatgagg ataaaccttc cttaggttat
      661 ggttcaacac ccaataattg gttaagccaa ttcttttcta cagagttata aaacacatcc
      721 tgatttatca agggtgataa atcagaaaag aaagtattac tacggagcag ttatgaaatt
      781 cccttcaata tttaataaaa taaatccaca atccatacag caacatccag aaaaaaatga
      841 acttaactgg atgctcgaat taaatcaatg gaaagcagaa cgcatactta caggtgaaat
      901 ccatcgtccg gaatgccgaa acgaagccgc taaaaggata aattgtgctt ttttgtcgaa
      961 acagaataat attgatttat cagggcttaa tttaactact caaccaccag gactgcaaaa
     1021 cttcacctct atcaatcttg atgagaacca actcaaacat tttgatgcaa ccacctacga
     1081 tagactcatc aaccttagtc tgaatagtaa tgctcttgag tcaataaatt ttcctcaagg
     1141 tagaaatgta agcattacac atatatctat gaataataat tctctcagaa atattgatat
     1201 agataggctt tcatcaatta cttattttag tgcggcacat aatcaactag agtttgtgca
     1261 attagaatgt tgcgaaaggc tgcagtacct gaatctcagc cacaatcaat taactgatat
     1321 tgttgcagga aataaagatg aactcttact tctggatcta tcccataata aactaacaag
     1381 tttacataat accttatttc ccaacttgaa tacgttactt atcaacaaca acttgctttc
     1441 tgaaattaaa atattcttta gcaacttctg caatattcag acattaaacg ctgcgaacaa
     1501 tcagttggaa aaaataaacc ttcatttcct gacttatctt tcatctatca aaagtttaag
     1561 gctggacaat aataaaataa cccgcattga cactaagaat acgtccgata ttggaacttt
     1621 attccccata ataaaacaga gcaaaaactt aaatttttta aatgtttctg ggaagaacaa
     1681 ttgccctact atgcagctca tgttatttaa tttattttcc ccagcactta agcttaatac
     1741 tggcctggca attctttcgc ctggtgcatt tgaaattcac tctgaaggag tagatgtgga
     1801 taacgaattg tttcactata ctatt
//
LOCUS       DAYPHN010000311         1797 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000311 DAYPHN010000000
VERSION     DAYPHN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.319"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..768)
                     /locus_tag="ACG0BW_004819"
     CDS             complement(<1..768)
                     /locus_tag="ACG0BW_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261654.1"
                     /translation="MSPVSSIGNDISSDLARRKMNDLPESPIGNNLEALAPGIEKLKR
                     TSIEMVTLLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGGIVDLSSLDLRN
                     VDLELYDFTDKHMANTILNPFKLDDTDFTNANMFQVNFVSSKQNATISWDYLLKITPV
                     ITSINDMYSEEKIKLVESCLNALGDITEEQLKIMRLAI"
     gene            complement(790..1329)
                     /gene="yhaB"
                     /locus_tag="ACG0BW_004820"
     CDS             complement(790..1329)
                     /gene="yhaB"
                     /locus_tag="ACG0BW_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFK7261655.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPTYFNKTSIP
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1585..>1797)
                     /gene="tdcR"
                     /locus_tag="ACG0BW_004821"
     CDS             complement(1585..>1797)
                     /gene="tdcR"
                     /locus_tag="ACG0BW_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFK7261656.1"
                     /translation="KFSNFIINKSFSAINNAARHIFSRYLLENKHLFYQYVKISNTGI
                     DHLEQLINVNFLPHDRTSFCECNRFP"
BASE COUNT          556 a          312 c          324 g          605 t
ORIGIN      
        1 aattgcaagc ctcataattt ttaattgttc ttcggtaata tctccaagcg catttaaaca
       61 actttcgaca agcttaattt tttcttcaga atacatatcg ttaatgcttg ttataacagg
      121 cgttattttt agcaaataat cccatgagat tgtggcgttt tgttttgagc taacgaaatt
      181 tacctgaaac atgttggcat tagtaaaatc tgtgtcatcc aatttaaaag gatttaaaat
      241 tgtattggcc atatgtttat ctgtaaaatc ataaagttct aaatcgacat tccgtaagtc
      301 cagagaggat agatcaacta tacctccagt cccacaacta aagattacgt tcaatctcct
      361 gatttcattg taaatgtctt tcaatgggca attaacattg atattgcctg tggatatttc
      421 ctggtttttt aacggttcat taattgtaag ttcacaggta ttgtctgatc gttgaataat
      481 tcgggcgtta taaccaaaaa aatccatacg aagagacgaa tcattataaa gaattacatt
      541 ttgtaagtac gctaattctt tttgaaaatc accagtgata acacatttcc cacctggttg
      601 taacgcgtta agtaaagtga ccatttcaat agaggtccgt tttagttttt ctattcctgg
      661 tgctagtgct tcgaggttat ttcctatggg gctttcagga agatcattca tttttcgacg
      721 ggctaaatca ctactaatat cattaccaat tgatgaaaca ggggacatta ttttatcctt
      781 tataaataat caataaaaat cattattaaa atgatcgggt gaaaaaaaga gaatttgcca
      841 acaatctgaa ggaatggagg ttttattaaa gtacgtaggg ggggttaaga tggcttcttt
      901 tcgggtgaat tttgcacaat acttagaaat cgtctcctta atacactcca gctctttttt
      961 attgacggct gagcaagtac attgttcgcc aaaaggtgca aaagtgccta catctaatgt
     1021 ttgtatgata taatcgtcat gttctcctgt agaaatatct atgatgaatg gactcatatt
     1081 ttccggttgg attcgaatca tgacagaagg aacatcgcct tgatagaaat tcaatgcagt
     1141 aattgcagat aatactctcc gcagttcatt acttatactg tatattttat taacgtcatt
     1201 ggtggagaca atttctgcaa actttctttt taccgtccaa taatcaatat ttctgttgtg
     1261 attgttaacc actgcgtgca ttggcgggat tgaagggtga aaaatatgtg caattgggaa
     1321 gcctttcatt gatgcctgcc ttgtcatcat gacaaacaat aatagatgct tattttcctc
     1381 gcagggttct ttcgcactat caaaaaacgc tcatttatcc ggcgttacag taaacgaagc
     1441 aacattctgt ggcctacgcc gatcaaaagc tctgtgaaaa ttccccttgt tgtctttggc
     1501 tgaccttatc gcggcgacca gcaattattc gttttgtgat cctcaacaca aaatgctatt
     1561 cgtcacctat gaggggagaa gaatttatgg aaaacgattg cattcacaaa atgacgtcct
     1621 gtcatgaggg agaaagttga cgttaatcaa ttgttcgaga tgatcaatac ctgtattcga
     1681 aattttaaca tattgataaa ataaatgttt gttctccaac aaatagcgtg aaaaaatgtg
     1741 acgtgccgca ttatttatcg ctgaaaatga tttatttata ataaaatttg agaactt
//
LOCUS       DAYPHN010000312         1797 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000312 DAYPHN010000000
VERSION     DAYPHN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.320"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..242
                     /locus_tag="ACG0BW_004822"
     CDS             <1..242
                     /locus_tag="ACG0BW_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261657.1"
                     /translation="LSQLSNESIRATSRYLESTTHAEKDIVEQAKNVMHSVMSSYLDQ
                     ISRDVQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
     gene            286..1635
                     /locus_tag="ACG0BW_004823"
     CDS             286..1635
                     /locus_tag="ACG0BW_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HFK7261658.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLGNSESEGNF
                     HNSASKDNNIGAMLKKDVEKLSTLVSECSPRNEDWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYLLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANVADNTTG
                     SIAMATTKGTLTHAIASLESSAPEVRSSETSSTLTASSSSEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALDLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNGQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          614 a          361 c          339 g          483 t
ORIGIN      
        1 tactttcaca gcttagcaat gaatcaattc gagcaacatc tagatatcta gaaagcacta
       61 ctcatgcaga aaaagacatt gttgaacagg ctaaaaatgt tatgcattca gttatgagta
      121 gctatctgga tcaaattagt cgtgatgttc aaggaagaga tcaggcactt aatttattac
      181 aacaaataat acaaaccatt attgatactg atagcgctat cgttcaaggc attcgctcat
      241 aaataattgc aataatagtt aattatattt aaggaggaat gactcatgat taataaagat
      301 aataatttaa atatagatat tctacgtcag cagaatgcaa acattcctct caaagaggca
      361 tcctcgcaaa gtgaggagcc tcatcaactg ggaaacagcg aaagcgaggg taattttcat
      421 aacagcgcca gtaaggataa taatattggc gctatgctca agaaagatgt tgagaaacta
      481 tcaactttag tcagtgaatg tagcccgcga aatgaagact ggaaatgcca gcacgacata
      541 caacttaact tcgttaaaaa catgctcaga aattttccaa ctgagaacct gtcagagaaa
      601 ggatccgcgc tcttacacga aagttatttg ctgataaaaa acgttctgca gcttacagaa
      661 aatgttaata ctattgacgg aaagttaaag gatcccgcta aaatattgac cctgcataac
      721 tctgtgagaa aaaccctggc taacgttgct gacaacacaa ccgggtcaat tgcaatggca
      781 acgaccaaag gaaccttaac tcacgctatc gcttctttag agagttctgc acctgaagtg
      841 agaagttcag aaaccagcag tactttaact gccagtagtt caagcgaatt taaaagtgat
      901 aaatatatat gcagtgatat tgctgatttg atgagtgtgc tcggtagcga ctacctcgaa
      961 atctatgcaa atagcgttga gataatgagc gcatactggc aggacttcag tgaacatatc
     1021 caaagcaata tgggaaagtg gactcactct aataaaaaag gcgatgcgat agtttttgat
     1081 gtcaatgcat ttcaaaaggc cttgatgcat ttttattata tcgataaata tcctaatggt
     1141 gatttccact atcattacaa tccagactat gttttatacc ctccggcacc agctgacaaa
     1201 attggtgttc cactggaaga agcagaaaaa tggtgcgccg ctttagatct tccggtaatt
     1261 ccacccgatc ccaaacaccg cacaccttct ccgattgtcg aagttgaacc gcaaggtagc
     1321 ggtctgtatg ttattattcc caaccctcag attattgaca gtatgagtca gtcttcagac
     1381 tcaatggtac acagggacga taaagggaag gaaaaaaata tatctaaaga attcaccggc
     1441 tacgaaataa gcacggctga ataccaggca tggctggcgg gttataacgg ccaggcagaa
     1501 aacatgaaaa ccgacgtgca ggttatcacg actaaataca gtacagccaa ctccacctac
     1561 gatacgatta tcaaattatt atcgtcaacc attactgcat tgtttgattc agcgaaggat
     1621 tatttacgtt tctgattaag taacacccac aattatcatc cactatctgg tcccgaaaaa
     1681 tgggaccagc gctttatatt attaagcatc acctttcatt catttcctca tcacaatctt
     1741 acaaaatgag cgttttttga tagagcaatt ctgccaatac tttattttat attgatc
//
LOCUS       DAYPHN010000313         1781 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000313 DAYPHN010000000
VERSION     DAYPHN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.321"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            112..783
                     /gene="queE"
                     /locus_tag="ACG0BW_004824"
     CDS             112..783
                     /gene="queE"
                     /locus_tag="ACG0BW_004824"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFK7261659.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1123..1700
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1123..1151
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          465 a          420 c          449 g          447 t
ORIGIN      
        1 gatctaatcc actttttgcc ttaaaactgc ctgtaacaga atgttaataa gacatgattg
       61 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      121 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      181 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      241 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      301 agtgataagt ggggggcggc gagcagtgaa gacttgctgg ctgtcattgg tcgccaggga
      361 tacaccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctgcca
      421 ctgactgacc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat
      481 gaggtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      541 ggctatgaag tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtggga
      601 cgcgtacgtg atattgaagc actggatgaa cttttggcga cgctgaccga tgataaaccg
      661 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgatt gtgcattgaa
      721 acctgcattg ctcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      781 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagatttc tactcattta
      841 tattgtttgt cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat
      901 tctctacaaa ttctgcggca ttaatttttc gctggagtga aaattattgc ggtaaagttt
      961 ggtagatttt agtcggtata ggattacttt aaatatttag cttttcaatc aatggattaa
     1021 gtgctcttta acataatgga tgtgttgttt gtgtgatact ataaagttgg tagattgtga
     1081 ctgacttaaa aaatcaataa ttaataatag gttatgttta gtgagttccc cgcgccagcg
     1141 gggataaacc gtctgggccg gattcgatcc cgcgcgtacc ggagagttcc ccgcgccagc
     1201 ggggataaac cgtacaatcc cgcatcgaaa ctgaataccc cgatgagttc cccgcgccag
     1261 cggggataaa ccgttgccca ggcttttgcg aaaatttgtg atttggagtt ccccgcgcca
     1321 gcggggataa accgacacgg ggcagattga gcaggactgc gacctcgagt tccccgcgcc
     1381 agcggggata aaccggcagc agatttacaa acagatcgga gattactgag ttccccgcgc
     1441 cagcggggat aaaccgcgcg gcctgcgctc gcgtaaaatc agttgcagga gttccccgcg
     1501 ccagcgggga taaaccgtcc gcgcaagcct atataaacca gattgatcag agttccccgc
     1561 gccagcgggg ataaaccgcg aaatcgcacg cgcttcccgc atgggggaaa gagttccccg
     1621 cgccagcggg gataaaccgt gtaaaagtct tggttttggc gcatcatttg tgagttcccc
     1681 gcgccagcgg ggataaacca ccatataacc cgttatctct ttctcaagtt tttatattag
     1741 cagtactcgt attaagcaat atatccacgt aacacctcat g
//
LOCUS       DAYPHN010000314         1776 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000314 DAYPHN010000000
VERSION     DAYPHN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.322"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            101..1243
                     /locus_tag="ACG0BW_004825"
     CDS             101..1243
                     /locus_tag="ACG0BW_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFK7261660.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          554 a          379 c          371 g          472 t
ORIGIN      
        1 ggaattataa ataaagccat aaaccttggt tccaaagctg gcaacaacta accagttaaa
       61 acatacgcat caaaatcaga taaacatttg aggtattgtt atgacaaata aacacccatc
      121 attgttttct ccttttatgc tgacagaaaa aataaagcta cgtaatcgta tcgttatggc
      181 tcctatgacg acctggtcag cgaatcctga tggaaccatc tctgagcaag aactcgaatt
      241 ttataaacgt cgctcacaaa atgttggttt agtaataaca ggatgtacct atgtcacgcc
      301 aagcggcatt gggtttactc atgaatttgc cgcgtatgac gaccgtttca ttaacagcct
      361 tgaaaagtta gcagcggcag ccaaaagtgg cggtgctccg gctattttgc agattttcca
      421 cgccggtaat aaagccatcc cggagcttgt accaaataac gatgttatta gtgccagcgc
      481 atcaagcgta aaatctggtg actttatgaa gagagtggta caaagcaggg aaatgactga
      541 aaacgaaatt caggaaacca ttagagcatt cggagatgtc acaaaaaggg cgattaaagc
      601 aggttttgat ggcattgaac ttcatggtgc gcacggattc cttctacaga atttcttctc
      661 ccctctattt aaccagcgta atgaccgatg gggaggagat ctcgaaagac gtatgcgatt
      721 cccgctggcc gtattacagg aagtaaaaaa tgtagtctat gaatatgcga caaagccctt
      781 tgccatcggt tacagaatat ctcctgaaga atctgcaaca ggggggctgc ggatagagga
      841 tacttataaa ctccttgatc ggctcattgc atccggtatt tcgtatatac atacctcgct
      901 ggtcagtatt aacgatagct accctgttga gtcgccaaat ggcccccgaa ctattgaatt
      961 gattttaaat catattgcag gcagagtgcc ggtaatcgct gcaggtaaaa tacgcacacc
     1021 gtctcaggct caagaagcaa tttccactgg cctgccactt gttgcgatcg gtaaagggct
     1081 ggtgataaat ccagaatggg tgactttagc tgaatcaggt agaagccatg aaattcaaac
     1141 aacccttaat ccacaacttg ttcctgaatt aaccattccg gataaactat gggatcaaat
     1201 tcaggcatcg aaaggaactg gctggtttcc gttaatggat taaatatcta ttactctccc
     1261 gagtgtaaaa ctatcaaacc aatgagtaac cgatacagcc tcgcaaattc tttcagataa
     1321 ttaacccgtc actgcggcac gttctctcat aaggcgtgcc tgctgtgtaa tccagtatgt
     1381 atccggcata gttgacggtt taatagaact caattcctct cccaggtcac caacaacgat
     1441 atcttgtata acaccccagg agatacgaca ttatgatgag atgcgatatg ctcctcagcg
     1501 ctaatcaacg ccaggactgg gcctgtcttg agatcagact cattcccgga actgacttca
     1561 ggcgacatat atcaatgacg ttggggcaat aaaagatagt tgatgtaata catctattca
     1621 aatttgtgat acatgtcaaa aaattggttg accaaactcg ttatttatat aagggcactt
     1681 acgaagtgca ctctttttta aagcgaggaa gtaccaatga aagagaataa agtacaacaa
     1741 atcagtcata aactgattaa tatcgttgtt tttgtc
//
LOCUS       DAYPHN010000315         1731 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000315 DAYPHN010000000
VERSION     DAYPHN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.323"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>1731
                     /gene="adeD"
                     /locus_tag="ACG0BW_004826"
     CDS             <1..>1731
                     /gene="adeD"
                     /locus_tag="ACG0BW_004826"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFK7261661.1"
                     /translation="SRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKG
                     RYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTT
                     VICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQML
                     AWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAG
                     IENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNP
                     WEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLL
                     SDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTP
                     GKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGL
                     NEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAG
                     LMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEK
                     FAFTTLEVTE"
BASE COUNT          375 a          470 c          491 g          395 t
ORIGIN      
        1 tagccgggcg gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
       61 tattattgat aatgtctcta ttctcgacct gatcaatggc ggagaaattt ccggcccaat
      121 tgtgattaaa ggacgttaca ttgctggtgt tggcgcagaa tacactgatg ctccggcttt
      181 gcagcggatt gatgcccgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
      241 tattgaatcc agcatgatga cgccggtcac ctttgaaacc gctaccctgc cgcgcggcct
      301 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggcgaag ccggtttcgc
      361 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccaa tacttgcagg tcagctcttg
      421 cgtacccgcc ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
      481 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
      541 aattagcggg cagaatgcgc tgctcgataa actggatgca tttcgccacc tgacgctgga
      601 cggtcactgc ccgggtttgg gtggtaaaga acttaacgcc tatattgctg cgggtattga
      661 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
      721 gttgatgatc cgcgaagggt ccgctgcccg caatctcaac gcactggcac cgttgatcaa
      781 cgaatttaac agcccgcaat gcatgctctg tactgatgac cgtaacccgt gggagatcgc
      841 ccatgaagga cacatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
      901 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
      961 cggcttactg gcacccggta agcaggctga tatcgtcctg ttgagcgatg cgcgtaaggt
     1021 cacggtgcag caggtactgg tgaaaggcga accgattgac gcgcaaacct tacaggcgga
     1081 agagtcggcg agactggcac aatccgctcc gccatatggc aacaccattg cccgccagcc
     1141 agtttccgcc agcgactttg ccctgcaatt tacgcccgga aaacgctatc gggtcattga
     1201 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     1261 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac gtctggctcc
     1321 ggcttgtggt ttgctcggcg gctttggtct gaatgaaggc gcgctggctg cgacggtcag
     1381 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     1441 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggac aggtccaaag
     1501 tcatctaccg ttacccattg ccggcctgat gagcaccgac acggcgcagt cactggcgga
     1561 gcaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttaccgg atgagccgtt
     1621 tattcagatg gcgtttcttt ccctgccagt gatccccgcg ctgaaactaa ccagtcaggg
     1681 gctgtttgat ggcgagaagt ttgccttcac cacgctggaa gtcactgaat a
//
LOCUS       DAYPHN010000316         1649 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000316 DAYPHN010000000
VERSION     DAYPHN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.324"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            126..1361
                     /locus_tag="ACG0BW_004827"
     CDS             126..1361
                     /locus_tag="ACG0BW_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFK7261662.1"
                     /translation="MAGENKLSDKALKGYLGKPREKQITIADGKGLSIRVSTKGAVSF
                     VFFYRLAGGRAAPVWLTLGKYPDMSLKQAREKRDECRGWLADKRDPRIQIKIQAEERL
                     KPVTVEDALNYWYENYCKVRRKTHAVTLGRFRKHIFPYIGHLPVNDTHLYEWLDCFDR
                     IKRNAPVMAAYVFSDTKLALRFCRVRQYATCDALKDLRMSDVGQIAGKRDRVLDEAEL
                     GQLWKAIFVEPDLKLMSEYTRKMFVLCTVFGCRMSEARLSEWSEWDLESWVWTVPKDH
                     SKTGVEIVRPVPEILRQWVTDVHEETKHTGYVLGSLRIRESVSKIGGKIGKRLGHEKQ
                     WSLHDLRRTLSTHLSDLGVEFYVVEQLLGHALPGVAGVYNRSKFMAKKLDALELWTTY
                     LNSIAGADSKVTILKQKAG"
     gene            1363..1590
                     /locus_tag="ACG0BW_004828"
     CDS             1363..1590
                     /locus_tag="ACG0BW_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7261663.1"
                     /translation="MKKMAIVDKKGLEYIPNIDRMIREKECRELTTLANSTRWKLEKE
                     GKFPKRIKIGATAVAYRLSEVQAWIRGEWRT"
BASE COUNT          480 a          295 c          429 g          445 t
ORIGIN      
        1 tttttacact taatcttttg ataataaaga ataagtttat ctggcgcttt cactgaattt
       61 tcctcgttat ctgtgtgttg caatcatctc tgtattgcag cttgtattgc tttttggggc
      121 taaaaatggc tggcgagaac aaactgagcg acaaagcgct taaaggatat ctggggaaac
      181 ccagagaaaa gcagatcacc attgctgatg gaaaggggct ttctattcgt gtgagtacta
      241 aaggggctgt gagctttgtt ttcttctaca ggttagcagg tggccgggct gctccggtct
      301 ggctaacgtt gggtaaatat cctgatatgt cactcaaaca ggcaagggaa aagcgcgacg
      361 agtgccgtgg ttggttggct gacaaacgtg atccgcgtat ccagattaag attcaggctg
      421 aagaacgctt aaagccggtc acagtggagg atgcactaaa ttactggtat gaaaattact
      481 gtaaggtgcg tcgtaaaact catgctgtaa cgcttggcag atttcgaaag catatctttc
      541 cctatatcgg tcatttgccc gtaaatgaca ctcacctata tgaatggctg gactgttttg
      601 accgaattaa acgtaatgca ccagttatgg cggcgtatgt tttttctgac actaaattag
      661 ctcttcgttt ttgtcgggta cgccagtacg cgacgtgtga tgctttaaag gatttgcgca
      721 tgagtgatgt ggggcagatt gcaggtaagc gggatcgggt tctggatgaa gccgaactcg
      781 gccagctctg gaaggcaatt tttgtcgagc ctgatttaaa actaatgtct gaatacacgc
      841 gaaaaatgtt tgtgctttgt acagtatttg gatgtcgaat gagtgaagcc cgattatcag
      901 aatggagcga atgggatctc gaaagttggg tttggactgt accaaaagat cactcaaaaa
      961 ctggtgttga aatcgtcaga ccagtacctg aaattctacg acagtgggta acggatgttc
     1021 acgaagagac aaaacatact ggttatgtgc tgggaagtct gcgaattaga gaaagcgtaa
     1081 gcaagattgg ggggaaaatc ggtaaacgtt tgggccatga aaaacaatgg tcactacacg
     1141 accttagaag aacgctatct actcatctaa gtgatctcgg tgttgaattt tatgtagtag
     1201 aacaactgtt aggccatgcg ctacctggcg tggcaggtgt ttacaatcgg agtaagttta
     1261 tggctaaaaa actggatgct ctggaactct ggactacata tctcaatagc atcgcaggtg
     1321 ctgattcaaa agtgacaatc ctcaaacaaa aggctggtta acatgaaaaa aatggcaatt
     1381 gttgataaaa agggtctgga gtacattcct aacattgatc gtatgatccg tgagaaagaa
     1441 tgtcgggagc taaccactct tgcgaacagc acacgctgga agctggagaa ggaaggaaaa
     1501 tttcctaagc ggatcaagat tggcgctact gcggtagctt accgtctctc cgaagttcag
     1561 gcatggatta ggggggaatg gcggacataa atgttgtagt aaattgttat ataaaagggt
     1621 gtaagcatgg ctactcgaaa aaaaaatag
//
LOCUS       DAYPHN010000317         1630 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000317 DAYPHN010000000
VERSION     DAYPHN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.325"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..587
                     /locus_tag="ACG0BW_004829"
     CDS             <1..587
                     /locus_tag="ACG0BW_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFK7261664.1"
                     /translation="NTNSLDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNF
                     PDLQYFKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNV
                     LESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVR
                     FNVNLKNQDKNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            601..840
                     /locus_tag="ACG0BW_004830"
     CDS             601..840
                     /locus_tag="ACG0BW_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFK7261665.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            860..1192
                     /gene="sctI"
                     /locus_tag="ACG0BW_004831"
     CDS             860..1192
                     /gene="sctI"
                     /locus_tag="ACG0BW_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFK7261666.1"
                     /translation="MSVSNMPPIDRAEQSAAREIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1189..>1630
                     /gene="sctJ"
                     /locus_tag="ACG0BW_004832"
     CDS             1189..>1630
                     /gene="sctJ"
                     /locus_tag="ACG0BW_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFK7261667.1"
                     /translation="MKYISLFLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHIS"
BASE COUNT          555 a          273 c          331 g          471 t
ORIGIN      
        1 tcaataccaa tagtttggat gaagctgtct gggtcaaaca agcactggaa aaaaatcaac
       61 caggaaagcc agtacgggtg ataaatcctg atgatgaatc gatacgaatt ttttcatggc
      121 ttgctgataa tttcccagat ctacaatatt ttaaactaca gctattagat gccagtaatc
      181 caagactgac agtgagtaag caacggaatg ctatcacaca gcaactaatc gataatctta
      241 ttaaaggatt actgcaaact atgccatatg ccagcaatat aagtattgcg gtattagatg
      301 ataatgtatt ggaaagtcag gcaattgaaa cattgtcggc aataggtctt tcttatgaaa
      361 aatataaaac agctaacaat gtatatttca atatcattgg tacgttaagc gacagtgaat
      421 taaataaaat taataactat gtcgacgaat attataaaca atggggtaaa caatatgtaa
      481 gatttaatgt gaatttgaaa aatcaggata aaaataatag ttcatttagc tacggagata
      541 accgattcga gaagtctcaa ggtagcaagt ggacatttca ggaataattg aggaggactt
      601 atggcagatt ggaatggtta tattatggat atcagcaaac aatttgatca gggcgttgat
      661 gatctgaacc aacaagttga aaaggcgttg gaggatttag caaccaatcc ttccgacccg
      721 aaattccttg ccgaatatca gagtgcatta gctgagtata cattatatcg aaatgcgcaa
      781 tctaacgttg taaaagcgta taaagatctt gattcggcaa tcatacaaaa cttccgctaa
      841 actggataag gattttatta tgtcagtaag caatatgcca cctattgata gagctgagca
      901 atcagctgct cgtgaaatac agcaagcgaa agttattgat ttaaatgacc gggttctcaa
      961 tcttgataac ccggatgata aaatgatctc cgcttttgcc aactatgcgg tacaaactga
     1021 aaattggcag caaaatgcgt tacaagcatt aaggtctgat aaaaaggggt taactcctga
     1081 aaaattatta gtcttgcagg atcatgtgct taattataat gtcgaggttt cattggtggg
     1141 aacattagca agaaaaattg tcgctgcagt tgagacgttg actcgctcat gaaatatata
     1201 tcattattct tgtttatcct gctgttgtgc ggttgcaagc agcaggaatt actgaaccat
     1261 cttgatcaac agcaagctaa tgatgtcctt gctgttttac aaagacataa tattaatgct
     1321 gagaagaagg atcaaggcaa aactggattt tcaatctatg tagaaccaac ggattttgct
     1381 tctgcggtgg attggctaaa aatctacaat ctcccgggta agcctgatat tcaaatatcg
     1441 cagatgttcc ctgcagatgc actcgtttcc tctccacgag cggaaaaagc aagattgtat
     1501 tctgctattg aacagcgttt agagcaatct ctcaaaatta tggatggcat tgtttcttca
     1561 agagtacatg ttagttacga tgttgataca ggtgatagtg gcaaaactgc tttaccaatt
     1621 catatttctg
//
LOCUS       DAYPHN010000318         1624 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000318 DAYPHN010000000
VERSION     DAYPHN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.326"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1227
                     /locus_tag="ACG0BW_004833"
     CDS             <1..1227
                     /locus_tag="ACG0BW_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFK7261668.1"
                     /translation="GRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAF
                     IQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYTSMLTQAMDYDRILAGSRSH
                     QTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMS
                     LTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRH
                     SMKASSDNNNFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGY
                     VKFGHGSTQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHRVSELPVNWWVQPSVIR
                     TFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSG
                     SSAEGYNGQATLNVTF"
     gene            1298..1493
                     /locus_tag="ACG0BW_004834"
                     /pseudo
     CDS             1298..1493
                     /locus_tag="ACG0BW_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT          363 a          445 c          471 g          345 t
ORIGIN      
        1 ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag tgcgtcgggg
       61 ctggcgacca gcggtaaggg tattcaggtg gtggaagcca ttaacggtgc caccacggag
      121 gaaggggcct ttatccaggg gaataagctg caggccggtg cctttaacta ctccctcaac
      181 cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc tgaagtcccc
      241 ctgtatacat ccatgctgac acaggcaatg gactatgacc ggattctggc aggctcccgc
      301 agccatcaga ccggtgtaaa cggtgaaaat aacagcgtcc gtctcagcat tcagggcggt
      361 catctcggtc acgataacaa cggcggtatt gcccgtggag ccacgccgga aagcagtggc
      421 agctatggct tcgtccgtct ggagggtgac ctgctgagaa cagaggttgc cggtatgtct
      481 ctgacgacag gggtgtatgg tgccgcaggc cattcttccg ttgatgttaa ggatgatgac
      541 ggttcccgcg ccggcacggt ccgggatgat gccggcagtc tgggcggata cctgaatctg
      601 gtacacacgt cctccggcct gtgggctgac attgtggcac agggaacccg ccacagcatg
      661 aaagcgtcat cggacaataa caacttccgc gcccggggct ggggctggct gggctcactg
      721 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtac
      781 acctggcagg ggctctccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      841 catggcagta cacaacatgt gcgtgccggc ttccgtctgg gcagccacaa cgatatgacc
      901 tttggcgaag gcacctcatc ccgtgacacc ctgcgcgaca gtacaaaaca ccgtgtgagt
      961 gaactgccgg tgaactggtg ggtacagcct tctgttatcc gcactttcag ctcccggggt
     1021 gacatgagca tgggtacagc cgccgccggc agtaacatga cgttctcacc gtcccggaat
     1081 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
     1141 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg ttataacggt
     1201 caggccacgc tgaatgtgac tttttgataa tcaggtcact ctccttcagg ccccggtcat
     1261 catgaccggg gctttttact ttcagggagc aacagatatg ccgggttgta catctcgttt
     1321 attgcccgaa ggccctttct cacgcgaaca ggctgttgcc gtcaaaacag cttaccgcaa
     1381 tgtgtttact gaagatgacc agggaacata ttcccggctg gttatccgta atgctgaagg
     1441 tcagttgcgc tggtgatgcc ggaacacagc ttaatactca gctcgccagt taagggcttc
     1501 tcagacaata aacgtcattc atttcatctg tcaggccgcg tcttctccgg gagacgtggc
     1561 tttttcattt attacaccag taaatcgtaa ccaccgataa ggagcaaatt atgcgactag
     1621 ccag
//
LOCUS       DAYPHN010000319         1609 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000319 DAYPHN010000000
VERSION     DAYPHN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.327"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(118..324)
                     /locus_tag="ACG0BW_004835"
     CDS             complement(118..324)
                     /locus_tag="ACG0BW_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7261669.1"
                     /translation="MLTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1083..1232
                     /locus_tag="ACG0BW_004836"
     CDS             1083..1232
                     /locus_tag="ACG0BW_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFK7261670.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          436 a          322 c          459 g          392 t
ORIGIN      
        1 gctcgtaacg ggtctgatac tgtggtttta ttttcatatt cctcacgggg gataacaggc
       61 agcagaacgc cgccggcagg ccggagactg cgtctgttgc agatgaacgg ttaagaatta
      121 cggacgggac tgtgcaatac gcgcctgcag ccaggcttcc acttcgcttt tcagccatcg
      181 ggagctgcgg cccattttga tgggcgcagg aaagtcacca tccttgatga gtttgtaaaa
      241 ccacttatcg gtcaggccgg tcagttgagt gataaatgcc atgtcgacca tctggtcatc
      301 catcagtgaa actggggtga gcatgagaat gttcctccgg atgttgttaa accagccggt
      361 gtggtcatga aaacaacgca ccaccttcac cggcataaca accggggaga aacgtaaaaa
      421 ataatcggga atgtgaccgc caggagataa acggggaggt tacggtggct gagccgtacc
      481 ggtatgccgg gtggttgtta cgaaacgatg cttccgcacg cgtgcgcgat aaagaaggga
      541 tgattttcgt gaggaagagg ggaaaactgg cgttctgata attgcagagt tatttacggg
      601 gcggttacga tggggcagac cgtgtccggt gggcaggcga caggtatcac cgatgggata
      661 agactgtctg attggtatta taaatatata agagagatca aacacatgag atcaaataca
      721 tgagttcata tatgtgaaat aaacacggtg agtgtgatca gtgggcatga tgagaacgca
      781 gtagcaacag actgcgctat ccctgatagc tggcgtataa cccgcgtata taaaacgtat
      841 ctgcaatgta cgtgaacaga gtgcattaag catccgtctg agcggtaaac atatccgacc
      901 tggtgagtga cagtctgaga gaccggttaa gtggtggctc gggagtaatg agtttggggt
      961 gggtaccaga atgccagtct ggcggtatgc tgacagtatc acgttaattg cagcataaag
     1021 tgatgagagg ctaatggaag ttaaaaccaa agcagttccg tcgttgttca tcccgggaga
     1081 cgctggaaaa agtgatttcc cacacgcgtt ataaacttac ctctgcggaa ctggaagcct
     1141 tcaactctgt ggttgatcac tggctggcag aactgacaat gaacaagctt tacgatcgtg
     1201 ttcccgcttc agtctggaaa tatgtcatct gagcgttcgg taatcacaac tgcatcgcgc
     1261 caggtgttat ccgctttgtg attcttattg gctcttctca ggttattaaa agctgaatat
     1321 gttcctgttg tcgggaacca ggataatacc gggaacgtta agcggtatag cgacaacaaa
     1381 gaattcgaaa tcaaaaggga atggtgccgg aggcgtgggc ggtaatgggc tgattttcag
     1441 gtattaccgg cttacagcgt gtggttgata ttgaggggct gtgagtttca cgtatttgtc
     1501 agaaacccag acgagttttc ctgtatggga cgaaataagg cagcggcaat acatggaaaa
     1561 atttaatatg gtatgaagga gaacctgtcg gttacgctta atataaccg
//
LOCUS       DAYPHN010000320         1608 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000320 DAYPHN010000000
VERSION     DAYPHN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.328"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..606
                     /locus_tag="ACG0BW_004837"
     CDS             <1..606
                     /locus_tag="ACG0BW_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFK7261671.1"
                     /translation="NKDSRINKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYDLIK
                     TLDVCNYDSETKVLTSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKH
                     KQHMDDRLIHLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKSKQKYGHYG
                     LGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQSH"
     gene            868..>1608
                     /locus_tag="ACG0BW_004838"
     CDS             868..>1608
                     /locus_tag="ACG0BW_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFK7261672.1"
                     /translation="MKLRTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSFQDDKISFTIETNHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWYSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGVNFLSQLLNIEDEVLNVLLR"
BASE COUNT          558 a          257 c          296 g          497 t
ORIGIN      
        1 aataaagata gtcgtataaa taaaaatgtt gtcgttctgg ctggaaatca tgaaatcaat
       61 tttaatggta actatagtgc acgactagca aatcataaac tctcttccgg tgatacatat
      121 gacttaatta aaacgctcga tgtttgtaat tatgactcag aaacgaaagt tttaaccagt
      181 catcacggaa taataagaaa tgaagaaaat gaatgttatt gtctaggtgc tttgcaggtt
      241 ccatttaatc agatgaaaga cccaattgat cctgaggaat tagcaaatat atttaataaa
      301 aaacataaac aacacatgga tgatcgtctt atccatctaa ttagaagcaa tgctatagca
      361 tccacacctg tatatgacaa ttattttaat aacactactg ctttcagacc aaaacctgaa
      421 gatatcttca aatgtggaca gacgctcaaa aaaagtaaac agaaatatgg ccattatgga
      481 ttaggggtag atcagcatca aaaaattgat aattatacca tggggctaaa ttcctggaag
      541 atagcaccta acgaacgtgg tgataaaaaa ggtgtcccag ggcttagttg ttttcaatct
      601 cattaaaact ctatgaatac ggtggtatat ctttttaatc ctgccagtat ttcatagttt
      661 ttcttaatcg attgtagagg catatactga tgacgcctct acaatcttta gggactttct
      721 ctactatgag ataagaaatt tcatattatg ctttaaggct gaatgatttg tgcaaaatat
      781 aattattttc ccgtcgaaca tgaggtgtat gtcataagat taaatgataa ctatgtattc
      841 cttgcggtga attatatgag gtaaaagatg aaacttagaa ctgtggcagg tactaacgac
      901 agtataacga ctatcgcaac aaatgatatg gtgcaagagc atgttactaa cttcactaaa
      961 gaactgtttg ggtatattgc aaatggtata ggtgatgata tatcaagtat tgccaggaca
     1021 atgctcggtg aagttgtgga aaaaattgat gattggcaaa tagaacgttt ccaacaatct
     1081 tttcaagatg acaaaatttc atttaccata gaaacgaatc attcagaaaa atatagtatg
     1141 ctatcgggca tgcgtgccca tattctacgt aggaataata gttatcagtt tattgtgaca
     1201 attaatagta aaaattacgg ttgccctctt gatgataccg atattaattg gtacagcatt
     1261 gtttatttac ttaataatat gacagtaaat gataatgcta atgatgttgc agttactgag
     1321 tcgtacaaac ccatctggaa ctggaaaata tcgcaatata atgtatctga tattaaattc
     1381 gaaacaatta taaaacccca gttcgctgac agaatctatt tctccaattg ttcacccgtt
     1441 gatcccacaa gtaccagacc aacctatttt ggtgataccg atggctcagt cggtgcagtt
     1501 ttgtacgcgc ttttcgcaac gggacatttg ggaattatgg ctgaaggagt aaattttctt
     1561 agccagttgc ttaatataga agatgaagtt ctcaacgttt tattaagg
//
LOCUS       DAYPHN010000321         1602 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000321 DAYPHN010000000
VERSION     DAYPHN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.329"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(19..522)
                     /locus_tag="ACG0BW_004839"
     CDS             complement(19..522)
                     /locus_tag="ACG0BW_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261673.1"
                     /translation="MVDIVEADKTDIYFIQESVYGKIGLPAFGNTIGPSAQQVVKKVF
                     AVVKERDKTHAKQRLLLEYNGNKLWMNAIDGSEAILPTEFSKRYELSLFNTTNFGEDP
                     FPDVNLYNNMKSSFFVRFGGTSHPEAWAIYNASTKEVKYIETAREIDKIFSDFNLSGT
                     LPIHIGR"
     gene            complement(642..>1602)
                     /locus_tag="ACG0BW_004840"
     CDS             complement(642..>1602)
                     /locus_tag="ACG0BW_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFK7261674.1"
                     /translation="QYGDFDNGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDK
                     DVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELINM
                     LDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFLLKELNNPE
                     HDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEP
                     EMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          442 a          370 c          269 g          521 t
ORIGIN      
        1 atcacacaat ttaaaaaatc atcgaccaat gtgaattggc aatgtaccac tcaagttaaa
       61 atcactgaat attttatcaa tttctcttgc agtttctata tatttaacct ctttcgtact
      121 tgcattataa atagcccaag cctcgggatg ggatgttcca ccaaacctta caaaaaaact
      181 tgatttcata ttattatata aattaacatc aggaaaagga tcttctccaa aattagtagt
      241 attaaataat gaaagttcgt atcgcttaga aaattctgtt ggtaggatag cttcactgcc
      301 atcaatagca ttcatccaga gtttgttacc attatattcc aacaataatc tctgttttgc
      361 atgtgtctta tctcgttctt taacgacagc gaataccttt ttaacaactt gctgtgcgga
      421 tggtcctatt gtattaccaa aagctggcaa cccgattttg ccataaacac tctcctgaat
      481 gaaataaata tccgttttat cggcctcgac aatatctacc atttatccct ccaaacaaga
      541 cctgactcca ggacaagagc atagtgtgta gtaaacactt catgctcttg ccctgaccat
      601 tgaaataaca actttcgttt gtttactgta ccagggcaag attacgaaag cccgctcccc
      661 gcaaggactg acgccaggta gtttctgtcc atggctgctt ttcgcatctt acgtcttaac
      721 cctgccttga ataccttatc attcgtcaaa atattaatag caatgtgccg tatccctgaa
      781 aataattctg ctgcgtttcc tcttcttatt ttgcagtcgt cttcattcat taccacgtcc
      841 agacgccagt gcagcttatt ctccacgtgc cagtggtttc ggattgctgt ggcgaacttc
      901 tctgcggtta aatcagcaga actgatataa tatctgaccg tcatttcggg ctctttcttt
      961 tgttctgcta ttattgaccg aaaggagact gccacgcata atttcttcag acctttccat
     1021 tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa caatgtgaag acggatttct
     1081 tctctgccgt gactcttttc actaattgcg taactgtcat gctctggatt atttaattct
     1141 ttcagcagaa atttttcctc gaaggcttta tttagccgcc cctggtttcc ttttacagcg
     1201 aataaataat cacctccctg tttttgtatc ttctctgcaa tatctttctg gcaacccatc
     1261 gcatcagttg tgatgatttt tcctttaata tccagcatgt taataagttc agggatagct
     1321 gtaatctcat tagatttctc atccgtcctg atctgtccga tgaccaggct gtgcattgtt
     1381 gagaacgcac taatgacatg aatcgctccc ctgcggcgac tcttgtcata agagtgtcgg
     1441 agcgtttttc catcaattgc aatgacgtct ttatcatctg aagaatggca gtcacgcatc
     1501 cagttaataa agcactcgtg aaattttgca ggactgatac aggatacaac tctggcaatg
     1561 gtatcgtgaa caggaatacc attatcaaaa tcaccatatt gc
//
LOCUS       DAYPHN010000322         1583 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000322 DAYPHN010000000
VERSION     DAYPHN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.330"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            68..283
                     /gene="essD"
                     /locus_tag="ACG0BW_004841"
     CDS             68..283
                     /gene="essD"
                     /locus_tag="ACG0BW_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFK7261675.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            288..599
                     /locus_tag="ACG0BW_004842"
     CDS             288..599
                     /locus_tag="ACG0BW_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFK7261676.1"
                     /translation="MTQKYELIVKGIRNFENKVTVTLALRDKKRFDGEIFGLDVAMDR
                     VEGAALEFYEAAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            596..1129
                     /locus_tag="ACG0BW_004843"
     CDS             596..1129
                     /locus_tag="ACG0BW_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFK7261677.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKNVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1126..>1583
                     /locus_tag="ACG0BW_004844"
     CDS             1126..>1583
                     /locus_tag="ACG0BW_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFK7261678.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRSISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQR"
BASE COUNT          441 a          339 c          423 g          380 t
ORIGIN      
        1 tctgattgat gtgtctggaa gcctacacat aataactatg ccatccgttc cgtgcggagg
       61 tgaggctatg aaatccatgg acaaaatttc aacaggcatt gcctacggca cctccgcagg
      121 cagtgctggc tactggtttt tacagctgct cgataaagtc acgccctcac agtgggcagc
      181 aataggtgtg ctgggtagcc tggtatttgg cctgctgacg tacctgacaa acctttattt
      241 caagattaaa gaagacaagc gtaaggctgc acggggagag taattcaatg actcaaaaat
      301 atgaactgat tgtgaaaggg atccgcaatt ttgagaataa agttacggta actttagcgt
      361 tacgggacaa aaaacgcttt gacggtgaaa tttttggcct ggatgtcgcc atggaccgtg
      421 ttgaaggagc tgcgctggag ttttatgagg cagcagccag aatgagcgtc cggcaagtct
      481 tcctggaagt agcagaaaaa ttgtcagaaa aagttgagtc ttatctgcag catcagtact
      541 cctttaagat tgaaaatcct gccaataagc acgagcgtcc tcatcataaa tatctatgaa
      601 cacaaaaatc agatacggcc tgtcggctgc cgttctggcg ctgattggtg ctggcgcatc
      661 tgctcctcag atacttgacc agtttctgga cgaaaaagaa ggtaaccaca caatggcata
      721 ccgcgatggt tctggcatat ggaccatctg tcggggtgcc acagtggtgg atggaaaaaa
      781 cgtttttccc aatatgaaac tgtcgaagga aaaatgcgac caggtcaacg ccattgagcg
      841 tgataaggcg ctggcatggg tggagcgcaa tataaaagtt ccactgaccg agccacagaa
      901 agccggtatc gcgtcattct gtccctataa cattggccct ggtaagtgtt ttccgtcgac
      961 gttttataag cggcttaatg ccggtgatcg taaaggcgca tgcgaggcga ttcgctggtg
     1021 gataaaggac ggtggacgtg attgccgcat tcgttcaaat aattgttacg gtcaggttat
     1081 tcgtcgtgac caggagagtg cattaacctg ctgggggata gaacagtgaa tcagatattc
     1141 acggtgattt tgctcgtgtt ggtaggattt gtcgtaggta atgtctggag cgacagagga
     1201 tggcaaaaaa aatgggcgga gcgtgatgct gccgaattat ctcaagaggt aaatgtccaa
     1261 tttgctgctc gaataattga acaggggcga agtatatccc gtgatgaggc tgttaaagat
     1321 gcacaacaga aagccgctga aatttctgcc agggctgctg atctgtctga tagtgttaac
     1381 cagctgcgtg ccgaagcaac aaaatatgcc atacgccttg acgcagcgca gcataccgca
     1441 aatcttgccg ctgccgtcag aggcaaaaca accaaagccg ccgaaggaat gctcaccaac
     1501 atgctcggag atattgcagc agaagctcag ctttatgctg aaattgctga caaacgctac
     1561 atcgcaggag tgacttgtca acg
//
LOCUS       DAYPHN010000323         1573 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000323 DAYPHN010000000
VERSION     DAYPHN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.331"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..121
                     /locus_tag="ACG0BW_004845"
     CDS             <1..121
                     /locus_tag="ACG0BW_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HFK7261679.1"
                     /translation="QGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            205..543
                     /locus_tag="ACG0BW_004846"
                     /pseudo
     CDS             205..543
                     /locus_tag="ACG0BW_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            623..1027
                     /locus_tag="ACG0BW_004847"
     CDS             623..1027
                     /locus_tag="ACG0BW_004847"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7261680.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            1024..1371
                     /gene="tnpB"
                     /locus_tag="ACG0BW_004848"
     CDS             1024..1371
                     /gene="tnpB"
                     /locus_tag="ACG0BW_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFK7261681.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1420..>1573
                     /locus_tag="ACG0BW_004849"
     CDS             1420..>1573
                     /locus_tag="ACG0BW_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFK7261682.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMN"
BASE COUNT          398 a          413 c          426 g          336 t
ORIGIN      
        1 gcaaggcaag aagctgttcc gctggcggct acccgaaggt aatccctatc tgaggacgga
       61 gaccagaaac acgtatacat cgcgctttac agaagtggca atggcgttcg ccagcattta
      121 aatggagagc cggatgcgct gaaaggtgca cgtccggttc ggggaggaga ggcagggaaa
      181 tagtccgact acgccctgcc tcttactcta ctcagcacat ggcagggggt cgtctatgtg
      241 gcgttcatca ttgatgtgtt tgccggatac atcgtggggt ggcgggtctc atcgtctatg
      301 gaaacgacat tcgtgctgga tgctctggag caggcgttat gggcccgtcg accgtccggc
      361 acagtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac acagcggctt
      421 aaggaagccg gattactggc atcaacagga agtactggtg actcgtatga caacgcgatg
      481 gcggagagca tcaatggcct ttacaaagcg gaggtaatac accgtaagag ctggaaaaac
      541 cgtaagcgtc gtctcagcac cgtctggcag atcctgaaat tcctgagaga atagtggaca
      601 ccaaatatgg tggacgctat ccatgaaatc attaaccgca gtgcgtaaaa aaagccctaa
      661 ttatcccgtt gagttcaaaa tcaaaatggt tgaactctcg catcgaccag agatctccgt
      721 agcgcaactc gctcgtgagc atgggatcaa cgataatttg ctgttcaagt ggcgccagta
      781 ctggcgcgaa ggaaaactac gtcctccttc aacaacagaa aacaacgtgc ctgagctgct
      841 cccgataact cttgatgccg aagatgttgt ccctacaacc tccccccggt cacaacctgt
      901 agctgctgcg acacctgaat cactcaatat cagctgtgaa gtaacgttcc ggcacggatc
      961 actccgtctg aatggtgcca tcagcgaaaa tatcctgaac ctgctgatac gggagctcaa
     1021 acgttgatcc cattaccatc agggacaaag atctggctgg tcgctggcat caccgatatg
     1081 agaaacggct tcaacggcct ggcggcaaag gtgcagacga cgctgaaaga cgatccgatg
     1141 tcaggtcacg tttttatctt ccgtgggcgt aatggcagtc aggtaaagct cctctggtct
     1201 accggcgatg gactgtgtct gctgaccaaa cggctggagc gcggccgctt cgcctggccg
     1261 tcagcccggg atggcaaagt gttcctcaca ccggcacagc tggcgatgct ccttgaaggt
     1321 atcgactggc ggcagcctaa aagactgctt acgtccctga ctatgttgta agcctcttta
     1381 tcctggtcga cgctgaatga gcctggtaat atacccggta tgagcagctc acttcctgac
     1441 gatatcaatg cactgaaacg tctccttgcc gaacaggagg cgctgaaccg tgccctgctg
     1501 gaaaagctga acgagcgtga acgcgaaata gaccatctgc aggcgcagct ggataaactc
     1561 cgccggatga act
//
LOCUS       DAYPHN010000324         1549 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000324 DAYPHN010000000
VERSION     DAYPHN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.332"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(921..1397)
                     /locus_tag="ACG0BW_004850"
     CDS             complement(921..1397)
                     /locus_tag="ACG0BW_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7261683.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          358 a          407 c          384 g          400 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtctgtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga tcgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcca ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAYPHN010000325         1544 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000325 DAYPHN010000000
VERSION     DAYPHN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.333"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(367..573)
                     /locus_tag="ACG0BW_004851"
     CDS             complement(367..573)
                     /locus_tag="ACG0BW_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261684.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            complement(869..1042)
                     /gene="gnsB"
                     /locus_tag="ACG0BW_004852"
     CDS             complement(869..1042)
                     /gene="gnsB"
                     /locus_tag="ACG0BW_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFK7261685.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDIKIDLI"
     gene            complement(1215..1445)
                     /gene="ynfN"
                     /locus_tag="ACG0BW_004853"
     CDS             complement(1215..1445)
                     /gene="ynfN"
                     /locus_tag="ACG0BW_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFK7261686.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(1477..1515)
                     /locus_tag="ACG0BW_004854"
                     /pseudo
     CDS             complement(1477..1515)
                     /locus_tag="ACG0BW_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          460 a          314 c          293 g          477 t
ORIGIN      
        1 cgtggcaggg acccccactg ggaggacccg acagcctgac aaccgtgacg agcatctgaa
       61 acagagcgtt acataatggc atggggataa ttcagaagga catcacagca tgctcacaca
      121 aattagtgtg agtgtcctgt ttcttactac gcgcaggact gacgagcatg aggggaaaat
      181 acgcgaaccg aaatgcctta tcttcagaaa tgcagccggc atccgaactg gacgatattg
      241 taaattcacc taaatacgag gcattgctgt agttgaattg cagtactgta ttagcattac
      301 agtgacagaa tacgatattg actctgtcac ttgtgaaatg gtcagaatgg ttagcagtta
      361 tggtgatcag tcaaccacca gggaataatc cttcgtattc ttatcgtgct ttactaacgc
      421 ggcctcaatt gccctgaatg cttccagaga tacttgatgt tctatacatg caattacaac
      481 atccgggtaa cccatcgaaa tggtgctatt aagcatagtt tttacacgaa cctgctcctg
      541 ggagggggca tcagtgtatt gatctttatg cattttgtcg ctccattcgt ttgctcttca
      601 cttgcggcta aaaatataac gtcaaatcac tcagcatgaa agagtgaaag tttcagcgcg
      661 tttcccttac ataatctgtc gtttgtattc tcttcaggca taccggaaag actaggtatt
      721 gtcacacaac caataccatc gacaatcgag atttatatct attcggtaat gtttaaatat
      781 aacaaaaccc cgtaaaaacg aggtttatgg ataatttgta ttgttgaata gcatctgatg
      841 agaaattgat gctaatacta taaacatgct agattaaatc aatcttgata tcatagcttt
      901 caagaccagt cattttttcc cgtgcagtaa actggatact ggtaacttct ttcccggtct
      961 ttttcttaag ctcaatgatt ttttttgtta tatattcaga aatatctgct tctgtttttg
     1021 tttttaactc ttcaatgttc atcatttcct cttttagtct gttatgactg tcctggtaca
     1081 cagtagtgtc aattatatgg agcaaacgta taaaagataa gatgaaacat cgcaataatc
     1141 aacatacgac ggtctaaatt tcacacaaac agataaagat gattatcatt tattatcaat
     1201 gcattagaat caaatcaatt caagagtctc attgctgctt ccagaatttc ttctgaagtt
     1261 acatgtcgat ccgctgctac ataaatgact ttatgatctc cggtcagaga tggaaaccct
     1321 gcagccatta cagtgaggtg tgttgtttcg ccatttggat attcacgcat gatggtgtta
     1381 actccggtca tcactggcac taccattgct ggttcagagt taaaaaaaac tattattttt
     1441 ttcataatgt taccgtaata tgtgagtatc catcgactag acactaagca aaaaagctcc
     1501 cgaaggagcc ttcattttca ctttcttaaa tctaacgaca gatg
//
LOCUS       DAYPHN010000326         1507 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000326 DAYPHN010000000
VERSION     DAYPHN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.334"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            75..1115
                     /locus_tag="ACG0BW_004855"
     CDS             75..1115
                     /locus_tag="ACG0BW_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFK7261687.1"
                     /translation="MIISQERWDKTFKNFEVYDCTYGFEHGRVGLLLIEKIDENHIEY
                     GNDYYMPKHKLVSIVIDNPPENRVFGMSFGNLSYPTISAGWSPSQTELVIVDIDCEVI
                     SYKTREYKGRENNLDIPISKSSDMRTAIIKVVRVGTSVYAIGGGLRIYKRINHQVWLD
                     ASQTIPVPDGFLERKAESMSRSLFQDLSGFSESDMYAVGGTGTVYHFNGEQWKKLPFP
                     TNKLLYTVCCAGDGFVYIADFDGAIWKGRNEQWTQITHGGMTMPFLDMGWFDGRLWCA
                     SDYGIWVLEDDKLVLAMHAKHKPVPPEVAVLSKRIDVSPDGTVMMVCGSRGAAIYDGN
                     AWNVLFDSMAFE"
     gene            1169..1483
                     /locus_tag="ACG0BW_004856"
     CDS             1169..1483
                     /locus_tag="ACG0BW_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HFK7261688.1"
                     /translation="METNVYINGREACSRASDGKSAGAFPDPCWSPPPPPTGPVVVPY
                     SNTAYARDLQNGTATVFICKSMVAQKDRSYFSTSEGNVPATPGFQKGHGWKTIPSILL
                     LQ"
BASE COUNT          439 a          288 c          363 g          417 t
ORIGIN      
        1 acacggatag catattatgg aaactaacgt ttatagatag ctatgatgat aattgtgata
       61 actaaggaaa tgaaatgatt attagtcaag aacgttggga taaaaccttt aaaaattttg
      121 aagtatatga ttgtacatat ggctttgaac atggccgcgt tggcttatta ttaattgaaa
      181 aaattgatga aaatcatatc gaatatggca acgactatta catgcctaaa cacaagctgg
      241 tatcaatagt tattgacaac cctcctgaaa acagggtgtt tgggatgtct ttcggtaatt
      301 tgagctatcc aacgatatcg gctggatggt caccttccca aactgaatta gttatagttg
      361 atatcgactg tgaagtcata agctacaaaa ccagagaata caagggacga gaaaataatc
      421 tggatattcc tatttctaaa tcctcagaca tgagaacagc tataattaag gtagtcagag
      481 taggaacaag tgtctatgca attgggggag ggttaagaat atataaacga attaatcatc
      541 aggtttggct ggatgcttca caaactattc ctgttcccga tggattcctg gaacggaagg
      601 ctgagtcgat gtccagaagc ttatttcagg atttatctgg tttttccgag tcagatatgt
      661 atgcagtggg tggaacggga accgtttatc actttaatgg tgagcaatgg aaaaaactcc
      721 cttttccgac aaataaactt ctgtacacag tttgctgtgc cggtgacgga tttgtttata
      781 ttgctgattt tgacggtgct atatggaagg gtcgcaatga gcaatggaca caaattacgc
      841 atggtggaat gaccatgcct ttcctcgaca tgggatggtt tgatggacgt ctctggtgtg
      901 ccagtgatta tggcatctgg gtactggaag atgacaaact ggtgctcgcc atgcatgcaa
      961 aacataaacc cgtccctcct gaagtggccg ttttaagtaa aagaattgat gtttcccccg
     1021 atgggacagt gatgatggtt tgtggctcca gaggagctgc tatttatgat ggtaatgcct
     1081 ggaatgtcct ctttgattca atggcgtttg aatagcctcc cgggccagca gttaatttaa
     1141 aggaaagtgg gtacacggat agcacattat ggaaactaac gtttatatca acgggcggga
     1201 agcctgctcc agagcgtctg acgggaaatc tgctggcgca tttcccgatc cctgctggtc
     1261 ccctcctcct ccgcccacag gcccggtcgt tgtgccttat tccaacacgg cgtatgcccg
     1321 ggatttgcag aacggaacag ctaccgtatt tatctgtaag tcgatggtgg cacagaaaga
     1381 tcgctcgtat ttctctacca gtgaagggaa tgttccggcg acaccgggat tccagaaagg
     1441 gcatgggtgg aaaacaatcc cgtccatact cttactccag taaaaacaaa tgaaaataat
     1501 gtgagat
//
LOCUS       DAYPHN010000327         1481 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000327 DAYPHN010000000
VERSION     DAYPHN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.336"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(16..309)
                     /locus_tag="ACG0BW_004857"
     CDS             complement(16..309)
                     /locus_tag="ACG0BW_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFK7261689.1"
                     /translation="MSLVRYNSKIIITVALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLLISQDRLTH"
     gene            complement(311..811)
                     /locus_tag="ACG0BW_004858"
     CDS             complement(311..811)
                     /locus_tag="ACG0BW_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFK7261690.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNTLLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCIRES"
     gene            1061..>1481
                     /locus_tag="ACG0BW_004859"
     CDS             1061..>1481
                     /locus_tag="ACG0BW_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFK7261691.1"
                     /translation="MVFMENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRVLVIV
                     GNDVSLGRSDSFSELPENTIVIPYGELTGSFEVIITTDPDLVVTIRELTAQESEDRTL
                     TLNQQIEVLGLKFAVKVKNEVWQYSIPGIVENNITSTR"
BASE COUNT          472 a          225 c          277 g          507 t
ORIGIN      
        1 gtgtagattc attatttaat gagttaaacg atcttgcgaa atgagaagca attgatacat
       61 actcatctct ttataagctg gaactgtagt aacctgtgca aattcgcgat atagcttttg
      121 ctccagttct gctgaagata tatattccgg gtgttcgcta aagaactgtc ttgcatctac
      181 tatcgcttgg gattcgccac ttcttttatt ttgattctcc tttaatgaat tttctctggt
      241 gcatgcagag agcaacgata tcagcgcgac agttattatg attttactat tatatcttac
      301 taatgacata tcaactttct cttatgcaag attggagttt aaaataatgt ttatcccatt
      361 cacagagtgc tataaacaac tcaacagtac cttttatgtt aagtttattt agggctctta
      421 ttttgtgata tgatactgtt ttataatcaa ttcctttgat aatggctatt tgttttaacg
      481 aataagaaac acataaccgc aggatatcta tctcatcatg ggtaagtggt tcttgagtgg
      541 aatacacatt ttgtgtatga agattcgaaa ccagaaaatg cgttaaatca gataatgtaa
      601 tattcttatt gatcctgatg aaattgttgc tcaaaagggt gtttacaggt tctgtatttg
      661 gtttgagatt tcgatgttgg tgctcatcaa ttacaacgaa cgaatgtcca agatcaacca
      721 acgcaaccaa atcgctgtaa tattcctttt gggatgctaa taatgcatta gtgcctacag
      781 tgaagtagac atcactggaa aagacttgca tatgccaacc tgcactctaa aatcatttag
      841 ggtaaatatt acgtgtaaaa tttaaaataa tggtaattgt tatatttgca tatcaatgag
      901 ggaatagggt gtatacctta aattgcaaat aaagttatgt atgtaggaat ttaaaatggt
      961 tctcgaaaat tataatcttt ttattgtgtg tcatcataat ttggtcatga aatttccgtc
     1021 ttgttacact actctaaact agagcttatg gaattagcgg gtggtattca tggaaaataa
     1081 tgataaattc ttatcacaag acttattgga atcttatgcc attcgtttat tgagcggacc
     1141 attgaatgga tgcgagtatg aaatacttaa tgggcgtgta ctcgttatcg ttggtaatga
     1201 tgtttcgtta ggaagaagtg attctttttc tgagttacca gagaatacga tagtcattcc
     1261 ttatggagag cttacaggca gttttgaggt aataatcact acagaccctg atctggttgt
     1321 aacaatcaga gaattaacag cacaagaatc tgaagataga acgttaacat tgaatcagca
     1381 aatagaagtt ttaggcctca aatttgctgt aaaagtaaaa aatgaagttt ggcagtattc
     1441 aataccaggt attgttgaaa ataacattac ttcaacaaga c
//
LOCUS       DAYPHN010000328         1480 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000328 DAYPHN010000000
VERSION     DAYPHN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.337"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            11..>1480
                     /locus_tag="ACG0BW_004860"
     CDS             11..>1480
                     /locus_tag="ACG0BW_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFK7261692.1"
                     /translation="MNTALSIIDDANSNTAIDYRQEMNVIHEIVAECEKEIAFMYQVH
                     DFVYGDERHNMINRLLQLNHRPDEERSRLNRAWLDKVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWREQFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYDIVHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKSGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNVGYQFDKIVMNP"
BASE COUNT          399 a          349 c          384 g          348 t
ORIGIN      
        1 tggtgcaatt atgaatacag ctctttccat catcgacgat gccaactcaa acactgctat
       61 cgattatcgt caggaaatga acgtcatcca cgaaattgtg gccgagtgcg agaaggagat
      121 cgccttcatg tatcaggttc acgacttcgt ttatggagac gaacgccaca acatgattaa
      181 tcgcctgctg caactgaacc accgaccaga tgaagaacgc tcacgtttga atcgagcttg
      241 gctggataaa gtcgatctgg aatgggtgaa acagaatatt tgggccgagt actggaggaa
      301 ggtcacggat atgactaacg ttctgctgat catgccagct tcccgtcgcg acgagtggcg
      361 tgagcagttt atcgagggta agcaggaagt catcaaaact gacagaaccg gctatcagat
      421 gaaggtaaaa gagttcgttg gtgtaccgga gttcaaagca gaaacggtca tacccacgat
      481 gcttaatttg ctgaatgaca ggcacaaata tctctctgag cgcgtgtatg gcttgtttaa
      541 ggcgctgagt cctgcgcaca agacaaataa gacaaacggt ttcagcgaac gtctgataat
      601 cgctgactgc atttctgatt tctggcggga cagcgttagt gtgaactatc gcaaagagga
      661 ctatatcgac gatctgcgtg tcttgcttca tttcttcgcg cacaaagaat ttattaccat
      721 caaccgcact gctgaggtgc tatcagctgc gtatcgggca aacgactgcc agaccggtga
      781 ctggatgaac gtcgatggaa atctgatgcg cgtgaagatg ttcaagaacg gcaacgttca
      841 ctttgaaata catcctgacg tggcctggaa gttgaatgag gtgctggctt acagtatgcc
      901 tgctgcaatc cccgcgccat gtcgcaccgc gccaaaaaca cgggcaccaa agcagttcgg
      961 gttaatccag aagacgatct ccgtgccggt tcgcactgcg cttcgtgacg ggcgattgag
     1021 caaagacaaa ggcgtatggt acttctctga ttcagctctc cagaagtcgc aggtggaaga
     1081 gcttgagcgc acactgagct tcattggcgg cgtgcaggag aaaaagcact ggcagttccc
     1141 gtatgacatc gtccatacgc taaatacgat tgtggctacc ggtttaatac cggatacaaa
     1201 atcacaccag ttctacccta ccccacgctt gattgctgag tacgttgcca gagccactga
     1261 attgaagtct ggtgagaagc tgctggagcc tcaagccgga cgtggggatc ttctggccta
     1321 tattaacgcc gatctggaag atgttacctg catagaaatc gcacctctct tcgctgatat
     1381 cctgcgtgga aaagggtata cgaacacgat ttgctgcgac ttcataaagt ggtctgagga
     1441 caacgtaggt tatcagttcg acaaaatcgt tatgaacccg
//
LOCUS       DAYPHN010000329         1478 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000329 DAYPHN010000000
VERSION     DAYPHN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.338"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..249
                     /locus_tag="ACG0BW_004861"
     CDS             <1..249
                     /locus_tag="ACG0BW_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFK7261693.1"
                     /translation="KILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDED
                     YEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            604..984
                     /locus_tag="ACG0BW_004862"
     CDS             604..984
                     /locus_tag="ACG0BW_004862"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7261694.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            981..1328
                     /gene="tnpB"
                     /locus_tag="ACG0BW_004863"
     CDS             981..1328
                     /gene="tnpB"
                     /locus_tag="ACG0BW_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFK7261695.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1378..>1478
                     /locus_tag="ACG0BW_004864"
     CDS             1378..>1478
                     /locus_tag="ACG0BW_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261696.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNR"
BASE COUNT          345 a          377 c          420 g          336 t
ORIGIN      
        1 aaaatcctgc tgcgatgtac ctggcttgac ccggcagaga aagaggcccg tcgtttgcat
       61 ctgcagggca tgacggttcc ggattatacg gtgaaggtga gaaagtggtt ttgtgtgatg
      121 tgtgacgaag attatgaggg ggaaaagtgc tgcccacgct gcggtaccgg ggtatacagc
      181 agggcgtggg ggcggcagga ggtgccgtcg gaagatgcca gggctgataa tccgttacag
      241 aggctgtaat ggttgcctcc ggagcagttt gcggtgatgc ctttcccggg aatggattgc
      301 cagtgcgggg actgtggagc ttaattccgg tgttggtggc ctttccattg attttatgcc
      361 aacagccccc tgtgactgac aggctgcctc gtcattccat tcgtatctcc agtaacaggg
      421 ggttgtattg tattttattg taccggttca ggtgtgcgtt tcccggcgcg tctgcaccgg
      481 cttaaccaaa ttcaacaggg ataaaatagt ggtaagcgta cagcctgaac cgtctggtca
      541 gaatctgacg aattagacaa agtggtgtcc accaaataag tagtgggaac caaagtgtca
      601 gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct gccctaatta tcctcccgaa
      661 tttaaacagc tgctcgttgc tgcctcctgt gaacccggga tatccatctc aaaacttgct
      721 cttgaaaatg gcattaacgc caatctgttg ttcaaatggc gacaacaatg gcgcgaggga
      781 aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct cgatgcagct
      841 gccgaacagc cagaatcgct cgcagaggac ccggaaaccc tcagtatcag ctgtgaggta
      901 acgttccggc acgggacgct ccgcttcaat ggcaatgtca gcgaaaagct cctgactctg
      961 ctgatacagg aactgaagcg atgatcccgt taccttccgg gaccaaaatt tggctggttg
     1021 ccggtatcac cgatatgaga aatggcttca acggcctggc tgcgaaagta caaacggcgc
     1081 tgaaagacga tcccatgtcc ggccatgttt tcattttccg gggccgcagc ggcagtcagg
     1141 ttaaactgct gtggtccacc ggtgacggac tgtgcctcct gaccaaacgg ctggagcgtg
     1201 ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag gcgcagctgg
     1261 cgatgctgct ggaaggtatc gactggcgac agcccaagcg gctgctgacc tccctgacca
     1321 tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata cgggcttatg
     1381 aacgacatct cttctgacga catcttcctg ctaaaacagc gcctggccga acaggaagcg
     1441 ctgatccacg ccctgcagga aaagctgagc aaccggga
//
LOCUS       DAYPHN010000330         1435 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000330 DAYPHN010000000
VERSION     DAYPHN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.339"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            64..363
                     /locus_tag="ACG0BW_004865"
     CDS             64..363
                     /locus_tag="ACG0BW_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold toxin-2
                     domain-containing protein"
                     /protein_id="HFK7261697.1"
                     /translation="MLGGEYTEGKAPTICLEGKDNRTGSHGALHSATGTLIEEKHFGK
                     EMSYKDARCMVIDEVALMYGCDKSCLKAQLDKAYQDAYTCGDLTLQKSMQVMGVL"
     gene            389..1429
                     /locus_tag="ACG0BW_004866"
     CDS             389..1429
                     /locus_tag="ACG0BW_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFK7261698.1"
                     /translation="MIIDIESYQKYLKGFYIYDCTYGYEHGKVGLSLIEVVDEEHAEY
                     ENPFYPPEKRLISITFTLSKKYRIYGRRATGISLMTISSGWPPSQTEYVMVSQDRDVM
                     SYKEDVYKGVEESINILIPGSIDMFSVIIKVVRIENSVYAIGSGFRIYKRVDHQIWRE
                     HSTYLPVPDGLLDRKVEAITRSRFRDLSGFSESDMYAVGGTGTVYHFNGEQWKQLPFP
                     TNKLLYTVCCAGDGFVYIADFDGAIWKGRNEQWTQITHGGMTMPFLDMGWFDGRLWCA
                     SDYGIWVLEDDKLVLAMHAKHKPVPPEVAVLSKRIDVSPDGTVMMVCGSRGAAIYDGN
                     AWNVLFDSMAFE"
BASE COUNT          443 a          246 c          340 g          406 t
ORIGIN      
        1 gatactccat aaggaagaaa aattaatata aagaatgccc cgtagcaaat atccaacaag
       61 aaagtgctgg ggggggaata taccgaaggc aaagcgccta caatttgtct ggaaggaaaa
      121 gacaatcgta ctggttcaca tggtgcatta cactcagcta caggcactct aatagaagaa
      181 aagcattttg gaaaagaaat gagttataaa gatgccagat gtatggtaat tgatgaggtt
      241 gcattaatgt acggttgtga taaatcttgt ttgaaagcgc aactggataa agcttaccaa
      301 gacgcttata cctgtggtga tttaactctg caaaaatcaa tgcaggtgat gggggtgtta
      361 taaacgatga agatatttga ggtgataaat gatcatagat atagaatcgt atcagaaata
      421 tctcaaaggc ttttatatat atgactgcac ctatggttac gagcatggaa aagtaggtct
      481 ttcattgatt gaagtggtag atgaggagca tgccgaatat gaaaaccctt tttacccacc
      541 tgaaaagaga ttaatttcca ttacgtttac cctctccaaa aaatatcgca tatatggtcg
      601 tcgcgcaaca gggatatctc tgatgactat atcttctggt tggcccccat ctcagaccga
      661 atatgttatg gtcagtcagg accgtgatgt tatgagttat aaagaagatg tatacaaagg
      721 agtagaagaa agtataaata tactcatacc aggttctatc gatatgtttt ccgttataat
      781 aaaagtagta cgaattgaaa acagtgttta cgctatcggt agtggtttca gaatatacaa
      841 acgtgttgat caccaaattt ggcgggaaca ttctacatac ctgcctgtcc ccgatggatt
      901 gttagatagg aaggtagaag caataaccag aagtcgattc cgagatttat ctggtttttc
      961 cgaatcggat atgtatgcag tgggcggaac gggaacggtt tatcacttta atggtgagca
     1021 atggaaacaa ctcccttttc cgacaaataa acttctgtac acagtttgct gtgccggtga
     1081 cggatttgtt tatattgctg attttgacgg tgctatatgg aagggtcgca atgaacaatg
     1141 gacacaaatt acgcatggtg gaatgaccat gccattcctc gacatgggat ggtttgatgg
     1201 acgtctctgg tgtgccagtg attatggcat ctgggtgctg gaagatgaca aactggtgct
     1261 tgccatgcat gcaaaacata aacccgtccc tcctgaagtg gccgttttaa gtaaaagaat
     1321 tgatgtttca cccgatggga ccgtgatgat ggtttgtggt tccagaggag ctgctattta
     1381 tgatggtaat gcctggaatg tcctctttga ttcaatggcg tttgaatagc ctccc
//
LOCUS       DAYPHN010000331         1374 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000331 DAYPHN010000000
VERSION     DAYPHN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.340"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            5..>1374
                     /locus_tag="ACG0BW_004867"
     CDS             5..>1374
                     /locus_tag="ACG0BW_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFK7261699.1"
                     /translation="MFSIKPGPRNLPIDNPTLLSWNITDGDLNSKLNTLEYLNCITNI
                     INSCGVYPQDLKDREIISTFHAEKVINDLLKNNYKISLFPDTTYRELNKAAQRSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPTGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRKNISEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIIDPVTKERDQIHPEDIHLTAKDFEASKDNISKDEWEN
                     LHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKN"
BASE COUNT          498 a          268 c          241 g          367 t
ORIGIN      
        1 tattatgttt tctataaaac caggaccaag aaatttacct atcgacaacc ccacattgtt
       61 atcatggaac attactgacg gggatctaaa ttccaaatta aatacattag aatatctaaa
      121 ctgtataaca aatattatta attcttgtgg agtttatcct caggatttaa aagacagaga
      181 aattatatca acttttcacg cagaaaaagt tattaatgat ctgttaaaaa acaattataa
      241 aatttccctt tttccagata caacttaccg agagttgaat aaagcagcac agcgtagcat
      301 tacagcgcca gacaggatag gagaaggaaa aacatgggtt tatcaacgag atacaatggt
      361 tgaaagaggt gataacagcg gtgttcatca gtatggtcca gctgaacatt ttactcacat
      421 tatatctgac aaaccgtccc caaaagataa gtatgttgca tatgctatta acattcctga
      481 ctatgaactg gcagctgatg tatataatat taacgtaacg tcacctaccg gccagcaaga
      541 aacatttaaa atattaatca atccagaaca tctacggcaa acacttgagc gtaaatctct
      601 tactgctgtt cagaaatcac aatgtgaaat catcaccccc aaaaaacctg gcgaagcgat
      661 tcttcatgct tttaatgcca cctatcaaca aatcaggaaa aatatatctg agtttgcacg
      721 ttgtcattat gggtatatac aaatccctcc cgtaaccact ttccgcgccg acggaccaga
      781 aactcccgaa gaagaaaaag gttactggtt tcacgcttat caacccgaag atctttgtac
      841 tatccataat ccaatgggag atttgcagga ttttatcgca ttggttaaag atgccaaaaa
      901 atttggtatc gatatcatcc ctgattatac atttaacttt atgggaatcg ggggtagtgg
      961 taaaaatgat ctggattatc cctctgccga tatacgagcg aagatcagta aagatataga
     1021 aagtggtatc cctggctatt ggcaaggtca ggtcttgatt ccctttatta tagatccagt
     1081 aacaaaagaa cgtgaccaaa tccatccaga agatatacat ctcactgcaa aagacttcga
     1141 agcaagtaaa gataatattt ctaaggatga atgggaaaac ctccatgcat taaaagaaaa
     1201 gcgtttaaat ggaatgccta aaacaacccc caaaagtgac caggttatta tgttgcaaaa
     1261 tcaatacgtt cgtgaaatgc gaaaatatgg cgtacgaggt ttacgttatg atgcagcaaa
     1321 acactcaaaa catgaacaaa tagagaggtc gataacccca ccgcttaaaa atta
//
LOCUS       DAYPHN010000332         1355 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000332 DAYPHN010000000
VERSION     DAYPHN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.341"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            347..610
                     /gene="ykgM"
                     /locus_tag="ACG0BW_004868"
     CDS             347..610
                     /gene="ykgM"
                     /locus_tag="ACG0BW_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFK7261700.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            610..750
                     /gene="ykgO"
                     /locus_tag="ACG0BW_004869"
     CDS             610..750
                     /gene="ykgO"
                     /locus_tag="ACG0BW_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFK7261701.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(785..1010)
                     /gene="ykgL"
                     /locus_tag="ACG0BW_004870"
                     /pseudo
     CDS             complement(785..1010)
                     /gene="ykgL"
                     /locus_tag="ACG0BW_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            1157..>1355
                     /locus_tag="ACG0BW_004871"
     CDS             1157..>1355
                     /locus_tag="ACG0BW_004871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261702.1"
                     /translation="MQCPGKEAIVPVTTGSLRLTSLNLALRGLTGQMTYLHLFNFSSL
                     IGMNTHNHFVRHVNPLKHFTDW"
BASE COUNT          387 a          298 c          283 g          387 t
ORIGIN      
        1 cacttcagtg atatttaaaa tatgcatcct ctcccttttt gtaagtaatt attatatccg
       61 tgggagagga atacacgctg tcaggtaatc aatcatactg cgataaatca tcggccagta
      121 aagtggagat aacctccatt ctcgaaaaat ccatactctc agcgaaacca tcatcaatca
      181 ctcatccagg cgtttatggg agcgtcagcc aatggctgtt aacaatgcca gacttccccg
      241 ttgaggaaat tccacatacc acaaatggtc acagggattg ggtgttgaaa tgatccggat
      301 gagcatgtat ctttacggtt atgttataac ataacaggta aaaatgatga aacccaatat
      361 ccatcctgag tatcgtactg tggtgttcca tgacaccagt gttgatgagt actttaaaat
      421 cggctcgact atcaaaacag accgtgagat tgagctggat ggcgtaacgt atccgtacgt
      481 gacaattgat gtctcttcta aatcgcaccc gttctataca gggaagctga gaacagtggc
      541 atcagaagga aatgttgcac gattcaccca acgttttggt cgttttgtta gcacgaaaaa
      601 gggggcatga tgaaagtcct taactctctg cgtaccgcaa aagaacgcca tccagactgt
      661 cagattgtga agcgaaaagg acggctatat gtgatttgta aatctaatcc acgttttaag
      721 gccgttcagg gtcgtaagaa aaaacgttga ttcaaaattc gacgaattaa cgatatttgt
      781 ctgattaata atcagaccgg attaatgttg gtgtatttat tacaccagca ttaattttcc
      841 tggggatgta tcttcctgtt catttaaggt caactgcctg acgtttctct ccgaatattc
      901 cattatatta atgttgactt gttgaccagg tgcgccctgt atgctggcat caactctctt
      961 ttagactgaa cacgccactc agtttcctcc ctttgcgacg cagcctgcat ttacactcaa
     1021 acggttaaga tgataaatgt ggtaaatctg ttggtactga cataaaaacg tttacgccac
     1081 aggaacggcc tgatccaccg gtaaccccat cgccgacgtt cgagtgccag ttagagtaac
     1141 gcgcacagat gactgaatgc agtgccctgg caaagaggcc atcgttcctg tgacaactgg
     1201 cagtcttcgt ttaacttcac ttaatttggc tcttaggggg cttaccggac agatgacgta
     1261 cttacacctg tttaattttt catcacttat tggaatgaac acccataacc attttgtgcg
     1321 gcatgttaat ccattaaaac actttactga ttggc
//
LOCUS       DAYPHN010000333         1345 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000333 DAYPHN010000000
VERSION     DAYPHN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.342"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            366..1190
                     /gene="gadX"
                     /locus_tag="ACG0BW_004872"
     CDS             366..1190
                     /gene="gadX"
                     /locus_tag="ACG0BW_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFK7261703.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCGEHFILVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAETLRKRALIFALLSVFLEDEQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQHALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLSNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          395 a          255 c          276 g          419 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccaacct ttaaaaaaca gttgggcatt
       61 cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata
      121 tgttatatga atgtttattc aatgtaacgg atatttttca tgtcactaca aggtattatt
      181 tgcttgctat taatttcact gcgttcttaa gcgcgccttt tgtattataa atccagtcat
      241 cctgcccgta gattatcatc ctgttttccc gcttcgttaa aatttattta tcaatcaatt
      301 tgacttaaga gggcggcgtg ctacattaat aaacagtaat atgtttatgt aatattaagc
      361 caactatgca atcactacat gggaattgtc taattgcgta cgcaagacat aaatatattc
      421 tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt tttgcggatg
      481 caagccaaat tcgagtagat aagtgtggtg aacatttcat attagtttca agggatacgc
      541 tttcattatt tctcccgatg ctcaaggagg aggcattaaa acttcatgca cataaaaaag
      601 ttccttcatt actcgttcat cactgtacca gagatattcc tgtttttcag gaagttgcgc
      661 aactatcgca gaataagaat cttcgctatg cagaaacgct acgtaaaaga gcattaatct
      721 ttgcgctgtt atctgttttt ctggaggatg agcagtttat accgctactt ctgaacgttt
      781 tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc gcccatgagt
      841 ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta aagaaaaaat
      901 tgcgcgaaga agagacttca tattcacagt tgcttactga gtgtagaatg caacatgctt
      961 tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc tgtggatatc
     1021 acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg cccacagagt
     1081 atcaggagcg atcggcgcag ggattgtcga accgtgactc ggcggcaagt attgttgcgc
     1141 aagggaattt ttacggcact gactgttctg cggaaggaat aagattatag agttttactc
     1201 agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg tctccagact
     1261 aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca gtaagttaaa
     1321 tataactttt actggaaata agatc
//
LOCUS       DAYPHN010000334         1345 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000334 DAYPHN010000000
VERSION     DAYPHN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.343"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..922
                     /locus_tag="ACG0BW_004873"
     CDS             <1..922
                     /locus_tag="ACG0BW_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFK7261704.1"
                     /translation="TIARVVSCISPAKFHECFINWMRDCHSSDDKDFIAIDGKTLRHS
                     YDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKIITTDAM
                     GCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKNHGR
                     EETRLHIVCDVPDELIGFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADL
                     TAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDK
                     VFKAGLRRKMRKAAMDRNYLASVLAGCGLS"
     gene            934..1345
                     /locus_tag="ACG0BW_004874"
                     /pseudo
     CDS             934..1345
                     /locus_tag="ACG0BW_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          428 a          252 c          317 g          348 t
ORIGIN      
        1 taccattgcc agagttgtat cctgtatcag tcctgcaaaa tttcacgagt gctttattaa
       61 ctggatgcgt gactgccatt cttcagatga taaagacttc atcgcaattg atggaaaaac
      121 actccggcat tcttatgaca agagtcgccg caagggagcg attcatgtca ttagtgcgtt
      181 ctcaacaatg cacagtctgg tcatcggaca gatcaggacg gatgagaaat ccaatgaaat
      241 tacagctatc cctgaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga
      301 tgcgatgggt tgccagaaag atattgcaga gaagatacaa aaacagggcg gtgattattt
      361 attcgctgta aaaggaaatc aggggcggct taataaagcc tttgaggaaa aatttccact
      421 gaaagaatta aataatccag agcatgacag ttacgcaatt agtgaaaaaa atcacggcag
      481 agaagaaacc cgtcttcata ttgtttgcga tgtccctgat gaacttattg gtttcacgtt
      541 tgaatggaaa ggactgaaga aattatgcgt ggcagtctcc tttcggtcca taatagcaga
      601 acaaaagaaa gagccagaaa tgacggtcag atattatatc agttctgctg atttaaccgc
      661 agaaaagttc gccacagcga tccgaaatca ctggcacgtg gagaataagc tgcactggcg
      721 tctggacgtg gtaatgaatg aagacgactg caaaataaga agaggaaatg cagcagaatt
      781 attttcaggg atacggcaca tcgctattaa tattttgacg aatgataagg tattcaaggc
      841 agggttaaga cgtaagatgc gaaaagcagc catggacaga aactatctgg cgtcagtcct
      901 tgcggggtgc gggctttcgt aatctttccc tgacaagcct ggaaagtgga acataaattg
      961 tcggatattc tactgttgac tatttgtgcc gttatttctg gtgcagaagg ttgggaagat
     1021 atagaggatt ttggggaaac acatctcgat tttttaaaac aatacggtga tattgaaaat
     1081 ggtattcctg ttcacgatac catcgccaga gttgtatcct gtatcagtcc tgtatcagtc
     1141 ctgcaaaatt tcacgagtgc tttattaact ggatgcgaga ttgccatact tcagatgata
     1201 aagacgtcat cgcaattgat ggaaaaacac tccggcattc ttatgacaag agtcgccgca
     1261 ggggagcgat tcatgtcatt agtgcgttct caacaatgca cagtctggtc aacggacaga
     1321 tcaggacgga tgagaaatcc aatga
//
LOCUS       DAYPHN010000335         1309 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000335 DAYPHN010000000
VERSION     DAYPHN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.344"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            381..899
                     /locus_tag="ACG0BW_004875"
     CDS             381..899
                     /locus_tag="ACG0BW_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFK7261705.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPLEA"
     gene            1120..>1309
                     /locus_tag="ACG0BW_004876"
     CDS             1120..>1309
                     /locus_tag="ACG0BW_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261706.1"
                     /translation="MSLKGLRFTLEIDGQEPDTFAVVHFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIW"
BASE COUNT          334 a          282 c          349 g          344 t
ORIGIN      
        1 ttaattgctg gcaatacatc ataaggaatt cctttaagga atgtcttaat tgcgtgctgg
       61 aacgatggct gagtaagtgt cgtaataatt tgtttttgac gcggctgtgg atttttatgg
      121 ggggaaggat acgttatgga taattctgct gttgaagttg atatattttt agcttatgtg
      181 accgtgttct gaaattgact aaaaatatat ttaaacacca taaaagagtt tttcagtagg
      241 tgctaattac gtgtgcctgt aactcgaata aagaaaaggg tgtggttcct gatgtgagcc
      301 gcatcacact ctaacccgtc ggggattaac gacgggttgt tcgtaaaaca gcagttgata
      361 atttcacaag gagttcataa atgccaaccc catgttacat ttcaattaca ggtcagaccc
      421 aggggaacat cactgccggt gctttcaccg cagattctgt cggcaatatc tacgtgcagg
      481 gacacgaaga tgaaatgctg gtgcaggagt ttctgcataa cgttactgtt ccaaccgatc
      541 cacagtctgg tcaaccttcc gggcagcgcg cgcacaaacc gttcattttc accgtggcgc
      601 tgaacaaagc cgttccgctg ctgtacaacg cgctggcctc cggtgaaatg ctgccgaaag
      661 tggagctgca ctggtggcgt acttcagttg aaggcaagca ggagcactac ttcaccacac
      721 gtcttactga cgcgacgatt gtggacatga acctgcacat gccgcactgc caggaccctg
      781 cacagcgtga gtttacgcaa ttgctcgcag tatcactggc ataccgtaag gttgagtggg
      841 agcacatcaa atccggtact tctggtgccg atgactggcg tgcaccgctg gaagcataaa
      901 tctgagtcaa caacatcctg ccttcgtgca ggatgttgtt tttgtacgtg taaaaaatga
      961 gtgtcagtaa ggagttacgc tttcatgagg gagatggcgg atgccggaaa cccgggcggt
     1021 agcgtacctg aaaatgtaaa gatgagtgta acgctgcact ggcacccttt gctgcatggt
     1081 aagaatcatt tggtccgtga ataaatcagg gaggtcatta tgtccttaaa aggtctgcgc
     1141 tttacgctgg agattgacgg ccaggagccg gacacctttg cagtggtgca tttcaggtta
     1201 atccagaacc agtcttatcc gtttgtgatg agtgtggatg ttgccagcga ctcttttatg
     1261 cagacggcgg agatgctgct ggagaagaag gcgacgctga ccatctggc
//
LOCUS       DAYPHN010000336         1252 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000336 DAYPHN010000000
VERSION     DAYPHN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.345"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..1175
                     /locus_tag="ACG0BW_004877"
     CDS             <1..1175
                     /locus_tag="ACG0BW_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFK7261707.1"
                     /translation="DASDNAASESLTADHEKRKTTEDGSSDIPTPSKMSLEEALEELD
                     SIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRSYDFIGSIDQAEAHIKRLIKTFS
                     DIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSSTLAKDLERAIASRNRVQFIPGQ
                     RRGRIHGSSIYRLTMNDDRVFRKKEESKAVNACVQQVIDLSGSMSGITIQLALASAYT
                     IADALDRINVPNIITGFTTFGSHMAAGELKAVKYEFSRFESLMLPIIKNWNEKVNSRE
                     VRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMEDRKIMLVLSDGAPWAVGRGFDAH
                     LRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALVTSVDSLGSSVVTELSRIILK"
BASE COUNT          381 a          254 c          287 g          330 t
ORIGIN      
        1 ctgatgcttc tgataacgcg gcgtcagaat cattaaccgc tgaccacgaa aaaagaaaaa
       61 cgacagaaga cggctcttca gatatcccaa ctccgtcaaa aatgagtctg gaagaggctt
      121 tagaggagct ggatagcata gaagatgaag tcggaggcat gacagaagat gctctatccg
      181 aaacgattaa aagcgagtta acagaaagct cgaaaagcga atacaggcca tacaatcgct
      241 catacgactt catcggctcg attgatcagg cagaagccca tatcaaacgg cttattaaaa
      301 cattctccga tattgattta ggtggatatc caatcagccg ttatcgcatc gttcctgaag
      361 gcaaccaact cttcgacaaa tatattgaaa agcacctttc gtcaggtgtt tcgtcgacgc
      421 tggcaaaaga cctggaacgt gcaatagcaa gcagaaacag agttcagttt atacctggcc
      481 agcgtcgggg gcgcattcat ggttcaagta tctacagatt aacaatgaat gatgatcgcg
      541 tgtttcgtaa aaaagaagaa tctaaagccg ttaacgcctg tgttcaacaa gtgattgatt
      601 tatcaggttc aatgagtggt ataacgatac aattggctct tgcaagtgca tataccatcg
      661 ccgatgccct tgatcgaata aatgttccca acattatcac cggcttcact acatttggta
      721 gtcatatggc ggcaggagaa cttaaggctg tcaagtatga gttctctcgc tttgaatctt
      781 taatgctacc tatcatcaaa aattggaatg aaaaggtaaa ttctcgcgaa gttcgctcac
      841 gtatggggtg cgtaggctac acattcccac ttcttaataa cgtggatggt gaaagcatag
      901 ccagccttgc atcgttattt tccggtcgca tggaggacag gaagatcatg cttgttctga
      961 gtgatggcgc gccgtgggct gttgggagag gttttgacgc tcatttgcgt tcagttgcga
     1021 agcaaattga aacgcagact gatattgatt tgatggcaat tggcatcatg actgacgcac
     1081 cggagagatt ttactcaaat catgccctgg taacgagcgt tgatagtctt ggttcatctg
     1141 tagttactga actatctcgt atcattttaa agtgaacaaa acagccttaa tgataagtaa
     1201 ccacttacga tagatgatga tatatttata taagaagttg aacgctcatt ag
//
LOCUS       DAYPHN010000337         1228 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000337 DAYPHN010000000
VERSION     DAYPHN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.346"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            55..558
                     /locus_tag="ACG0BW_004878"
     CDS             55..558
                     /locus_tag="ACG0BW_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFK7261708.1"
                     /translation="MRLPKVKILSIFMFSILITKMSFASSACFNEAGSIFRIEPNLIK
                     AIALVESNLKNDSIGKNRDKKNNIKSVDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMENKKKRLQYAKKIYVVYTRLNEID
                     KRKALAK"
     gene            complement(577..1209)
                     /locus_tag="ACG0BW_004879"
     CDS             complement(577..1209)
                     /locus_tag="ACG0BW_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFK7261709.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELIKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          408 a          224 c          196 g          400 t
ORIGIN      
        1 tactgaatat attagtatct cgttcctttc cttcaatcct acacataaaa atatatgcgc
       61 ttacccaaag ttaaaatctt atccatcttc atgttttcta tattaattac aaaaatgtct
      121 ttcgccagta gcgcttgctt taatgaagca ggatcaattt tcagaatcga accaaatttg
      181 atcaaagcta tcgctcttgt tgaaagtaat ttaaaaaatg atagcatcgg caaaaacagg
      241 gataagaaaa acaatataaa gagtgttgat tatggtttaa tgcagataaa ccagatgcac
      301 attcccatgt taaaaaaacg tggaataata aaagatgaac gagatttgtt agataacccc
      361 tgtctgaaca ttaaaatagg gaccgagatt ttatataatc atttttcacg ttgtggggta
      421 acgtggcaat gtctggggac atataacgcg ggttttgcaa tggaaaacaa aaaaaagagg
      481 ctgcaatatg caaaaaaaat atatgttgta tataccagac ttaatgaaat agacaagcgt
      541 aaggctttag caaaataagc ctaaagcctc ttttttttat atagtgcaaa cacccatacg
      601 taaagctgtt ttgatcaact cgataccact gtgaacatct aatttcttca tgatattgag
      661 tctatgagtt tcgactgttt tcctgcttaa ctgcaagaaa ttagcgattt ctttgtttgt
      721 ttttccatca gctatgagtt gtagtatttc agactcacga tttgtaagtt gattatcaga
      781 ggaatatctt gaagatattt tttcacaatc catgtgtaca ctatcaaaat aagtgtaacc
      841 attactaatc gaatcaatag cgcgatttag ttttgccggg tgactggttt tccatacgca
      901 tccttttgca ccggcttcaa gtaattttat tgaatcaata tacggcattt ttgctacaaa
      961 tgtaataata tgagcatcta ttttatgcga tttaacccat ttaatgagtt cggaccccat
     1021 cattccacct gcaaattctc ctaaaataaa tatttgggct gcagatttgt tacactccgc
     1081 gatagctttc ttcagatctt tatagccccc aacaacctca tataaatcgg gataatgtcc
     1141 gagaaatcca attatcccat caatcataaa cggctgctga tctgaaacta caattttaat
     1201 ctttcccata tattcatcct gagtgtaa
//
LOCUS       DAYPHN010000338         1216 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000338 DAYPHN010000000
VERSION     DAYPHN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.347"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            16..>1216
                     /locus_tag="ACG0BW_004880"
     CDS             join(16..310,310..>1216)
                     /locus_tag="ACG0BW_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFK7261710.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEQNTIFKKG
                     YRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSE
                     IRRTWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVA"
BASE COUNT          326 a          305 c          333 g          252 t
ORIGIN      
        1 gccacctgat taaaggtgat atcctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gtactttcac ccctgaattc aggctggaat gtgcacagct aattgttgat
      121 aagggctact catatcgaca agccagtgaa gcgatgaatg tcggttctac cacgcttgag
      181 agttgggtgc gccagctcag gcgagagcgt caggggattg cgccctctgc cacacctatt
      241 actccagacc agcaacgtat ccgcgaactg gaaaagcagg ttcgccgcct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttgatgtccg actcgctgaa cggttcacga
      361 tagttgccag actgagtgac agccactcag ttgtcagcct ctgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcgcgagtca
      481 ggttgtgcag cgaaatacgc cggacgtgga accaaagtag gggctctgcg ggggcgcgca
      541 cgctggctga aatgctgacc caaaacggcg tcccgatgag ccgttaccgt gccgggcgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtac aaaaatgctc
      661 gtcaggaaca tacctgtctg ccgaatctgc ttgagcgcca gttcgctgtg cccgagccag
      721 atagggtatg gtgcggagat attacgtata tctgggcagg aaatcgctgg tgctatctgg
      781 cggtcgtcat ggatcttttt gcccgcaggg ttatcggctg gagcctgtca gcgaatgccg
      841 atactgccct gataagcagt gccctgcgga tggcgtatga ggtgcgtggt cagccgcggg
      901 acgtcatgtt ccatagcgac cagggaagtc aatatacagg actgaaatat caacaacttc
      961 tctggcgtta caggataaag caaagtgtca gccgacgggg aaactgctgg gataacagtc
     1021 caatggaacg cttcttccgc agtctgaaaa cagaatgggt gccaaccgat ggctacacag
     1081 gcaaagacgt ggcccggcag caaatcagca gttatattct gaattattac aatagcgtca
     1141 ggcctcacca ttataacgga gggctgacgc cggaagagtc agagaacaga taccattttt
     1201 actgtaaaac cgtggc
//
LOCUS       DAYPHN010000339         1202 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000339 DAYPHN010000000
VERSION     DAYPHN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.348"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            191..1099
                     /locus_tag="ACG0BW_004881"
     CDS             191..1099
                     /locus_tag="ACG0BW_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFK7261711.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
BASE COUNT          336 a          277 c          270 g          319 t
ORIGIN      
        1 aaatcaacac tttgtttgtt gttgtatttg tgatgcatat cgccatttat ttattgaatg
       61 ttcgaaaaca tactttttta atgcaatgtt ttttcgctat acccctttat agattctgaa
      121 ttctcacctg tcggctattt tatcctcacc tcatgtgaca tattccccac tttactcaag
      181 gaagattcat atgaaagcac ctgtttcaat aacctcattt cgcccacaaa aatctattgc
      241 tatcgtcatt ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa
      301 tgaaggtgaa cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgagccggg
      361 tctgggtttt aagatccctt ttatggaaag cgtggagaaa atttccacgc gtaatcaggc
      421 tgtggtttat cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt
      481 atcagtcagc ttccacatta aaccttcaga agcaggcgcg gtttatacca cctacaacac
      541 cattgaagca ttaaaagatc gtctgattgt ccgtcagttg ccaactcaac tggagaacgt
      601 tttcggccaa tataccgcca tcagtgcggt acaggatcgt acaaaattgg ttcaggatct
      661 gcaaaatgct atgcgtaaag ccgttgttgg tccggtggtg attgatggtg tccagattga
      721 aaatattgat ttttctgacg cttatgaaaa atcgattgaa gatcgaatga aagcagaagt
      781 cgccattgcc acacgtaagc aaaatctgga aacagagaaa atccaggcgc aaatagcggt
      841 aactcaggcc caggcagaag cagacagtaa actggcggca gccaaagcag aagctgagac
      901 cattcgcgta agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc
      961 agaagcaatc cgcctgcgcg gtgaagctct gcgggataat cccggacttg tagcccttac
     1021 cactgcagag cgttgggacg gtaaactgcc cgataccatg atccccggca gtacagttcc
     1081 gtttatctct actaagtaaa aaaagggccg ctttcgcggc ccttgaaact gatggcaaag
     1141 gcaaaactgc ctgatgcgct acgcttatca ggcctacata cttcctgcaa tatattgaat
     1201 tt
//
LOCUS       DAYPHN010000340         1201 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000340 DAYPHN010000000
VERSION     DAYPHN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.349"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            316..>1201
                     /locus_tag="ACG0BW_004882"
     CDS             316..>1201
                     /locus_tag="ACG0BW_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFK7261712.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETLSVLDNHKSIKSES
                     HSKYKHDNNDSDDSDNSES"
BASE COUNT          368 a          261 c          262 g          310 t
ORIGIN      
        1 ttgaatgaac accactattt ccagcctaat agctcttgag atcggacacg tacaaaaatt
       61 agctgatgag tgtgtagctg acatcctcac cgagctaccg aatgagcaga ttgaggttgg
      121 tgtgaatgac acaactggct ttatattcga acttaacaac aaacgcttca cgcttatcaa
      181 taccggctcc gggtctttag ccgtcagaac ctgttaatcc ctcttctacc tgcgcgaatg
      241 gcttagttcc ctgttcgcgc agtgctacat taaacacact agtaaataat ttgttttcat
      301 aacaaaggat tagccatgtc taaaaaacgt tccatcaaag aggttcagga cttccgtgac
      361 agtgtaaaac gagtagtcgc tctcctttca ggtaaaaaca tccctgttgc agaacgaggg
      421 gacgacgctt atgtacgcta taacgatgat ggagagccaa ttctcgtaaa catcccatca
      481 atcccggata acgcaacacc ggcattgatg aatgctgtgc gcggatttct cgatcatgag
      541 gttgctcaca ttttgtttac cgatattcgt gtgtccaaca aaatgagaga aaaaggacgc
      601 gttccttcct ggtcgctatg gaatgcctta gaagacgtgt tcatcgagcg aaaaatgggt
      661 caggtcttta acggaacaag acgtaatctg atggcaactc agcgccttat aatcgaaaaa
      721 gtctttaaac caaaggcttc agaggctatt gcttattgtg gcaaagatca gcgcgcgctt
      781 tttctaaact tctttctctg tccggttgta agagcctggg atggccaagc accgttcgta
      841 gatttcatgg atgaatattg gcctgtcatt gagaaaccaa tttcattatt aaaagaacat
      901 ggtatcgatg tggccgtgcg taacatgtct tgcaccgagg attgtgtaaa ggtggctgcg
      961 accatagcta agatcctcaa agacactgaa agtgaaagca aaggtaagga gtcagctccg
     1021 ggaaaaactt ccgatccttc agacgctgac cagacggatg cctctggaga aaacaatgaa
     1081 gacaacgaag atcatgagac actctcagtg ttagataatc acaaatctat caaatcagaa
     1141 tcacatagta agtacaaaca tgataataac gacagtgatg attcagataa ttctgaatca
     1201 t
//
LOCUS       DAYPHN010000341         1198 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000341 DAYPHN010000000
VERSION     DAYPHN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.350"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(1..541)
                     /gene="papA"
                     /locus_tag="ACG0BW_004883"
                     /pseudo
     CDS             complement(1..541)
                     /gene="papA"
                     /locus_tag="ACG0BW_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
     gene            complement(749..1062)
                     /locus_tag="ACG0BW_004884"
                     /pseudo
     CDS             complement(749..1062)
                     /locus_tag="ACG0BW_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
BASE COUNT          357 a          302 c          200 g          339 t
ORIGIN      
        1 gttgcgactg cagaaaaagc accctcagtg acttgggcat tattggctga cgacttctta
       61 acaatcgcag tataatgcag cacattatca ccatctttca gggtattagc atcaccttct
      121 gtgccatcga aagaaacgtt tttacctgca gcctgaacta caatcgcagt acctgtcccc
      181 ccgctggtat ctaactcctc attatgacca gaaacccttg gaccactaaa agccagcttc
      241 acagtccctt tttttgctgc cgagactcct cccccccctt aaatgaagtg atatcacaat
      301 taaccaattc aatatctaaa ttcattggtt tggatacgcc tcttgcttga aggaagcttt
      361 ttgaaagctg tccaaaatca atagactgat cagctgattt ctgagaaatg ctgcatggag
      421 catcaacaac agttccgtta aaggttactc taccctgccc ctgtggagta gttagagcag
      481 catttacacc aaaagacacc actgccatag ctaccgcacc ggcaataacc gacttaatca
      541 taaataacaa cctctttttc attactgaac acaccagaga tatctggcaa tttaaaatga
      601 cccgattacc tttaataacc agatcaatac aaataatatt ctcgtatttt cagggcaggt
      661 tatttatcga cagaatactg atataacctg tctgctttct gacaagggct tcttttttta
      721 tcttccagaa actccggaat gccataattt agtcaaatgc cgatgactca tctgtataat
      781 aaggtgcaag ccttgctgca agagcattca gccgtataag tctccccagt gttgtactga
      841 aatacccatt attcatctgg tatttctcgc agacctcctt acgggagtgc ccacctacca
      901 gatagtcctt catagccaga atgaccctgt cactgtgaat agaagaaata cctatcagta
      961 aaaaaaatgc atttcagaca tagagccgag caacagcacg ctctcgcgta tattcagcaa
     1021 aaacgcattt cctgaccgac tgatgacttc atgatgcgcc atgtttcccc cttctgtcgg
     1081 gcccctggat atatgcttcc ggcccggaaa tactcttcac gcaataagtt aaatttagtt
     1141 ttttatgttg taaatattga tttaaacgat cttttaaccc acaaaacaaa attaaact
//
LOCUS       DAYPHN010000342         1194 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000342 DAYPHN010000000
VERSION     DAYPHN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.351"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(151..1113)
                     /locus_tag="ACG0BW_004885"
     CDS             complement(151..1113)
                     /locus_tag="ACG0BW_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261713.1"
                     /translation="MGTGFCSNDNQQKIRSKFNIEESYCAIKTNGVLGFSNRRSAWRA
                     NGDSTGAISSTNAMMLMANGENEISLEIGALGWFSDKPASMEERGRFFPKAGCSLDLV
                     RFIKQEETLLSSIKVTINQQGIPEARPDSVHPVIRKEILAEQAEPGFIDPDYFDETYF
                     PKGMKVYQFTQKVTVTGLPEWAWTRATPYTGSDEQLRKLKAAYTEMASIISSRDRARL
                     KAYNKEALKAWSATTGDSEDDILLSLFSKDNVEGGKARMQPIRWDDYAVRVMNGGRMV
                     QLYNKSKPIYSPLTYRFTDESGEERMGYYAPVFSLIDGQFIPVT"
BASE COUNT          308 a          305 c          236 g          345 t
ORIGIN      
        1 gccggtacct ttgtcgttta atctggcagc taaaggcata ttgttttcct gtctggaata
       61 tcagcgacaa aaaatttaaa aaacggtaag gtgcttaact cagtaataat caccttaccg
      121 gacatcctga tttgtagccc aacaggttaa tcaagtaaca gggataaact gaccatcaat
      181 cagcgaaaat accggagcgt aataccccat acgctcttca ccactttcat ctgtaaaacg
      241 gtaagtcagt ggggaatata ttggtttcga tttattatat agctgcacca tacgtccacc
      301 attcataacc cggacggcat aatcgtccca cctgatgggc tgcattctgg ctttccctcc
      361 ctccacatta tcttttgaga agagtgagag taatatgtca tcctcagagt caccggttgt
      421 ggctgaccat gccttcagcg cctctttgtt ataagctttc agacgtgccc tgtcccggct
      481 actgatgatg cttgccattt cggtataggc ggcttttaac ttccgtaact gctcgtcgct
      541 tccggtatag ggggttgccc gcgtccaggc ccattcagga agccctgtca cggtaacttt
      601 ttgagtgaac tgatacacct tcatcccttt cgggaaatag gtttcatcaa aataatctgg
      661 atcaataaat cctggctccg cctgctcagc caaaatctct ttccggataa cgggatgcac
      721 gctgtctggc cgggcttcag gtatgccctg ctggttgatg gtcactttta ttgaagaaag
      781 cagcgtttcc tcctgtttaa taaaacgaac caaatccagg ctgcatcctg cttttggaaa
      841 aaaacgcccc cgttcttcca tactggcagg cttgtcagaa aaccatccta atgccccaat
      901 ctccagactg atttcatttt ctccattcgc catcagcatc atggcattgg tgctggatat
      961 ggccccggtt gaatcaccat ttgcccgcca ggcacttctt cggttactga acccgaggac
     1021 gccattagtc ttaatggcac aatatgactc ttcaatatta aacttactgc gaattttttg
     1081 ctggttatcg ttagagcaaa atccagttcc cataacaact cctgacagta aaataaccga
     1141 tattaaatgt tttgccttca taattattct ctctcaaaag gggaaaatac tcat
//
LOCUS       DAYPHN010000343         1190 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000343 DAYPHN010000000
VERSION     DAYPHN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.352"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..211
                     /locus_tag="ACG0BW_004886"
     CDS             <1..211
                     /locus_tag="ACG0BW_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFK7261714.1"
                     /translation="MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRK
                     LLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(222..293)
                     /locus_tag="ACG0BW_004887"
                     /pseudo
     CDS             complement(222..293)
                     /locus_tag="ACG0BW_004887"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            353..502
                     /locus_tag="ACG0BW_004888"
                     /pseudo
     CDS             353..502
                     /locus_tag="ACG0BW_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            529..633
                     /locus_tag="ACG0BW_004889"
                     /pseudo
     CDS             529..633
                     /locus_tag="ACG0BW_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            948..>1190
                     /locus_tag="ACG0BW_004890"
     CDS             948..>1190
                     /locus_tag="ACG0BW_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261715.1"
                     /translation="MTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAV
                     NALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSA"
BASE COUNT          394 a          221 c          243 g          332 t
ORIGIN      
        1 gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc acgttatcag
       61 caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac atctggcaag
      121 gctgggacgg aagttactgt cgttctcaaa atcggtggag ttgcatgaca aggtcatcgg
      181 gcattatctg aacataaaac actatcagta agttggagtc attacctcct cccgaactca
      241 aagctatcat acagataatt actggttaac cctatatagg gcattttaaa gtgttgtgat
      301 cttacccagc aatagtggac acgcggctaa gtgagtaaac tctcagtcag aggtgactca
      361 caagacaaaa cagtttcaac cagtaaaaaa acgtaaacac cattcgcatg aatttcgtag
      421 ttaagatctg aagcttgcag aatgcatcgg tgttactgcc ggagtccgtg aacttagtct
      481 gcatgaatca ccacagcttt acatctgtcg cagtaaacag caaaatcagc aaacttactt
      541 aattatatcg agtgcgatta caatcgctgg cgatggtaca gttggtgtgg cggtctcagt
      601 ccggaacaat ttgaaaatca gaatctcact taaaacggca tccattttac aaaagatggc
      661 cgtctgtgta tttataatga ataccttcta catgtttaga gtgcataatc cacgacaagg
      721 cgatatccta aaaattagct agcaggcaac tttgatggca tagataatgt ttttagtaaa
      781 aaagagttga ctgaatataa tacagcctct tcctacataa gaaggaatgt gctcacaaaa
      841 aaaaccatta gtctgcttcc tgttcttaca ataatcagta ttatgtattt tatttatata
      901 gcaaaatatg cttgttacac ctgtcataat taggcatggt aaaattattg actgaaccct
      961 gggggagaaa gatgcacagt gattattata atatggtttt tggtgaaaaa ttggccaata
     1021 ttttatatga agctaactct caattttttt acgagagaaa tgtcatagaa gaggcagtca
     1081 acgctttatt ctgtgagcgt gaaataataa acaacaagaa cataataaag aagctgatgt
     1141 tctttttgtc agatgttaat cacaccaaga aagatgttgt tcagagcgca
//
LOCUS       DAYPHN010000344         1179 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000344 DAYPHN010000000
VERSION     DAYPHN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.353"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..298
                     /locus_tag="ACG0BW_004891"
     CDS             <1..298
                     /locus_tag="ACG0BW_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261716.1"
                     /translation="KNGKYHLTETLDFRSENIKKQMGEAAISVLTITKAYELYNSNID
                     SFVIFAAETTAQEKSAKQQLNLLEKHADNLINAYSKEDMRSYYEKMLNAASMLQ"
     gene            799..1005
                     /locus_tag="ACG0BW_004892"
     CDS             799..1005
                     /locus_tag="ACG0BW_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFK7261717.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1082..>1179
                     /locus_tag="ACG0BW_004893"
     CDS             1082..>1179
                     /locus_tag="ACG0BW_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFK7261718.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELA"
BASE COUNT          360 a          289 c          239 g          291 t
ORIGIN      
        1 aaaaaatggc aaataccatc ttacagaaac attagatttt cgttctgaaa atataaaaaa
       61 acaaatgggt gaagctgcga taagtgtttt aacaattact aaagcctacg aattatacaa
      121 ttcaaatatt gattcatttg taatttttgc cgctgaaacg acagcacaag aaaagtcagc
      181 aaaacagcag ctaaatctac tggaaaaaca cgctgataat cttatcaacg catatagcaa
      241 agaagacatg cgttcatatt acgaaaagat gctgaatgct gcctctatgt tacagtagaa
      301 aaaactgact aatttaatta ataacccggc caccgtgccg ggttttcttt tgtcccctcc
      361 tctcatcaca caccgctcaa aaaaccacca cactactgct tcagttatcg ctatgcgatg
      421 caagtcacaa aattaattct ttttgctatc aaacatttaa tatcaaaaca cattaaccaa
      481 tagcaataag tattgatatc accaatagca atagctatta tcaccatatc gcaacaacac
      541 aacgatacgg caaccacctg attcaccgtt gcgatgaccg cttagatccg cagcttgaat
      601 ttcagcaggc tccggggagt gcgaggggtg aagcggacgc gtgaacgtcg gtgtgaccag
      661 ctgaaatcaa cacaacactt tatacctcag tcgcttcaac gaggcggctt agttatgaca
      721 accggcggcc atccaccgcc tgaatacgcg cagaagtctc tatatgttca gcagcccagc
      781 ttacgggcag gagtttttat ggttcatcaa cattacggaa cgcagaccgt taatcgcggc
      841 gcggtcatgc caggaatgct ggtcaaacac aaagatggta cctggactgc atcagctaat
      901 ttacgcggac ggctttatct gcatcgcggc atcgagcgca cttatacccg tgatttgctc
      961 gtggaagttt ttctcgacgg acgcggtaac ggcctgaatc actaaccccc ctttcctgtt
     1021 ttcctaatca gcctggcatt tcgcgggcgc tattttcaca gccattttca ggagttcagc
     1081 catgaacgct tattacattc aggatcgtct tgaggctcag agctgggcgc gtcactacca
     1141 gcagatcgcc cgtgaagaga aagaggcaga actggcaga
//
LOCUS       DAYPHN010000345         1178 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000345 DAYPHN010000000
VERSION     DAYPHN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.354"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            14..730
                     /gene="yeeN"
                     /locus_tag="ACG0BW_004894"
     CDS             14..730
                     /gene="yeeN"
                     /locus_tag="ACG0BW_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFK7261719.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          385 a          209 c          237 g          347 t
ORIGIN      
        1 ggagatcatt ccagtgggac gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga
       61 cggtgcaacg tctaaaattt atgcaaaatt cggtgtagaa atctatgctg ctgccaaaca
      121 aggtgaaccc gatccagaat taaacacatc tttaaaattc gttattgaac gtgcaaagca
      181 ggcacaagtt ccaaagcacg ttattgataa agcaattgat aaagccaaag gcggtggaga
      241 tgaaacgttc gtacagggac gttatgaagg ctttggtcct aatggctcaa tgattattgc
      301 tgagacattg acttcaaatg ttaaccgtac gattgctaac gttcgcacaa ttttcaataa
      361 aaaaggcggc aatatcggag cggcaggttc tgtcagctat atgtttgaca atacgggtgt
      421 gattgtattt aaaggaacag accctgacca tatttttgaa attttgcttg aagctgaagt
      481 tgatgttcgt gatgtaactg aagaagaagg taatattgtt atttatactg aacctactga
      541 ccttcataaa ggaatcgcgg cgctaaaagc agctggaatc actgagttct caacaacaga
      601 attagaaatg attgctcaat ctgaagttga gctttcccca gaagatttag aaatctttga
      661 agggcttgtt gatgcccttg aagatgacga cgatgtacaa aaagtttatc ataacgtcgc
      721 aaatctctaa ttatatgtta aagaaatctg tctttatggc agatttcttt aatctcatat
      781 aatccttata aaaatataat gtttaactcg tcatattgct tataaaaaat ccccgttccc
      841 agagaaataa tatttattaa aatggcaggt cttctttttc tgattaccga agacaaagtg
      901 gcaattacgt atagtttccc gataaagacg cgacagctac atcccgcata aggcattttt
      961 ctctttatct ttgtacggta cttcatggaa cagaattttt gaccttgtga atcgtgatgt
     1021 ctgttgggga ggagcaattt gctcactgaa gcgtgagact cgattaagcg cacgaaacac
     1081 agaaatcaaa aaacccggcc acttttttac aaactgaccg ggtaaaaata atttttattt
     1141 tttaactgcc ttgagactca tagagatgtc tcaaaact
//
LOCUS       DAYPHN010000346         1169 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000346 DAYPHN010000000
VERSION     DAYPHN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.355"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            243..318
                     /locus_tag="ACG0BW_004895"
     tRNA            243..318
                     /locus_tag="ACG0BW_004895"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:276..278,aa:Ile,seq:cat)
     gene            1075..>1169
                     /locus_tag="ACG0BW_004896"
     CDS             1075..>1169
                     /locus_tag="ACG0BW_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFK7261720.1"
                     /translation="MLVSKSNEINTSTFINSGNCNERKSSKSMELL"
BASE COUNT          388 a          199 c          229 g          353 t
ORIGIN      
        1 tattattaga tacaaaatca ttacaaacta tcattgaatc gatggtaact ataaatagtg
       61 ctaagtatta gcatttgctt gttgactgag agtatcgact ttgtgaatgg aatcggtgtc
      121 gggttttatg tcgcaggtaa tgacgaccca ggatgaatga gaacgtataa aaatctgtta
      181 tttacatcat aagatcatca tgtttattgc gtggtatata agtattgtta ttgcatactc
      241 aaggcccttt agctcagtgg ttagagcaga cgactcataa tcgtccggtc gctggttcaa
      301 gtccagcaag ggccaccaaa tcattattca cgtatcagga agagttaagg ttacaagggg
      361 gactcgatgg cggtcaatat ttcacgacta acgccatttt aaagaagcag tgcggtaggt
      421 agtttcgaat gagggctggt gttttggtac gtggcacgcc actgcaatgt atttatccat
      481 cagtactcat tcaaaagtac ctggaaaaaa tgcccgctat gccaagcggg caaattgctt
      541 acatgtggaa ttaagtaatt aacgataaag ccagagtatt atcatttatc atgcctcttc
      601 atttaatcag gttaattact caaatcactg aactattctc tgttcgctca ctgatgataa
      661 gtgaagtgtc acaaccacga tttgtatgtg gaccaatgct gtagagtaag gatgaaatgt
      721 ctctgccacg ccttaaacat ctactactga ctagaaccgc cgtcgaatgc agaagagtct
      781 tgtgagcatt acaggagtct ggctttggtc attaatcaac gctatttaga tataaatttc
      841 taacactcaa aaaacgattc tcacgaataa attataacgg gtggttttta tcatacagtg
      901 aaataacctt ttgcttatta aatttaacat tcttgtgcta aattaattaa aagtaccatg
      961 tatagcatat gaagctgtta atgccagcaa ttgtctcaaa tgagcgattt tttatagtga
     1021 caaacaataa taacattaaa aatagccatg tagacattaa ctgaggaaaa gacaatgtta
     1081 gttagtaaaa gcaacgaaat taacactagc acatttataa atagtggaaa ttgtaatgaa
     1141 agaaaatctt ctaaatccat ggagttact
//
LOCUS       DAYPHN010000347         1158 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000347 DAYPHN010000000
VERSION     DAYPHN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.356"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..165
                     /locus_tag="ACG0BW_004897"
     CDS             <1..165
                     /locus_tag="ACG0BW_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFK7261721.1"
                     /translation="IIEQAPTYEINEPIFAYDKTMPVSILLALMSIATCWLLWKYRAI
                     ITNLIRLKNK"
     gene            181..762
                     /locus_tag="ACG0BW_004898"
     CDS             181..762
                     /locus_tag="ACG0BW_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFK7261722.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNARISNPVLRSITNEMILLQ
                     YNLSVEHFSLNSSLIYYINNWKLFPLICLLSGCHFYRERFAERGFFYKVPDVLRNYLS
                     AIPVEINEKARYKPGIVNYQNIITCGFSTLLPYLRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKLSCKWCCD"
     gene            704..>1158
                     /locus_tag="ACG0BW_004899"
     CDS             704..>1158
                     /locus_tag="ACG0BW_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261723.1"
                     /translation="MRKKIEMSLINSPANGVVIKRIISDGLKEIVSLKEQILLETTAK
                     IQSIEEKREEKVIQGYYDGYAKGIIDVMDNFIPLISLLSSELEKNRINMINDLKSILL
                     KSSEEVEVFIKIFESWVTKLPSISGPLNLYIPTSFKDKSIEVESYFVDKS"
BASE COUNT          398 a          180 c          203 g          377 t
ORIGIN      
        1 attattgaac aagcaccaac atatgaaatt aacgaaccta tttttgctta tgataagact
       61 atgccggttt ctatcttact tgcattaatg tccattgcga cttgctggct gttatggaag
      121 tacagggcta ttataactaa tttaatcagg ttaaaaaata agtagacata tgagaacgtt
      181 atgaatcttg ccctaagaaa aattatatat gatccaatta gttatattca tccccaacga
      241 gtaagtttga ataatgcacg tattagcaat ccggtactta gatctattac caatgaaatg
      301 atactcctac agtacaatct ttcagttgag catttcagtc tgaatagttc acttatttac
      361 tacataaaca attggaaatt atttccattg atctgtttac tcagtggttg tcacttttat
      421 cgagaaaggt ttgcagaacg cggatttttt tacaaggttc ctgatgtgct aaggaattat
      481 ttatcagcta taccggtgga aataaatgaa aaggcacgat ataaacctgg tatcgttaac
      541 tatcagaata tcatcacatg tggtttttca acgttgttgc cctatctaag gcaacaaccg
      601 ttggcaatgc agcaacgatt taatttatta tttcctgatt ttgttgacca catacaattg
      661 ccgttaccac ttgcgtctac tcttttggag agaattacat tttatgcgaa aaaaaataga
      721 gatgagcttg ataaactctc ctgcaaatgg tgttgtgatt aaaagaataa tttctgatgg
      781 attgaaagaa atagtttctc taaaagaaca gatccttctt gaaaccaccg caaaaattca
      841 gagtattgag gaaaagagag aagaaaaagt tattcaaggt tattatgatg gctatgcgaa
      901 aggcattatt gatgtgatgg ataattttat acccttgata agtttgctga gctcagaatt
      961 agaaaaaaac agaataaata tgataaacga tctgaagagc atattgttaa aatcttcaga
     1021 ggaagttgaa gtttttatca aaatttttga aagttgggta acaaaattac cgtcaatatc
     1081 aggcccttta aatctctata tacctaccag tttcaaagat aagtctattg aagtagagtc
     1141 atattttgtc gacaagtc
//
LOCUS       DAYPHN010000348         1157 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000348 DAYPHN010000000
VERSION     DAYPHN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.357"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            49..567
                     /locus_tag="ACG0BW_004900"
     CDS             49..567
                     /locus_tag="ACG0BW_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFK7261724.1"
                     /translation="MMKFKKCLLPVATLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            583..1122
                     /locus_tag="ACG0BW_004901"
     CDS             583..1122
                     /locus_tag="ACG0BW_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFK7261725.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWIAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
BASE COUNT          366 a          251 c          297 g          243 t
ORIGIN      
        1 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
       61 aaaaaatgtc ttctgcctgt ggcaacgtta gcgtcattca ctctggcagg atgccagtca
      121 aatgctgatg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
      181 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
      241 aataaacgaa acgcacaagc cttcggcgcg cttattggtg cagtcgctgg cggtgttatt
      301 ggccacaacg tcgggtctgg cagcaattcc ggaacgacgg caggtgcagt tggcggcgga
      361 gctgttggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
      421 ttaacgtata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
      481 tttacgacag gtttagccgt tgttattacc acgacgtata acgaaacgcg tattcagcca
      541 aatacgaaat gtcctgaaaa gagctaatga tcaggaggag tcatgaagaa agtttttctt
      601 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
      661 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
      721 tggatcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaacgcccag
      781 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
      841 ctggatgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
      901 agcttagaga ttcagaaaca aaaactggcg ctggcgaaag aagaagcccg cgttaagcgc
      961 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
     1021 tctgaagctg acgcaaacag gaatatgact gagggcggtc gcgatctgat gaaaagcgtg
     1081 ggcaaagcag aagagaacaa atcggacagc tggtttaact aagcgatgtc agtaacttca
     1141 agcctatgat tcgtgag
//
LOCUS       DAYPHN010000349         1151 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000349 DAYPHN010000000
VERSION     DAYPHN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.358"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>1151
                     /locus_tag="ACG0BW_004902"
     CDS             <1..>1151
                     /locus_tag="ACG0BW_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFK7261726.1"
                     /translation="TVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVR"
BASE COUNT          277 a          329 c          345 g          200 t
ORIGIN      
        1 actgtggttt atgccggcgg cgaccagact gtacatggtc acgcactgga taccacgctg
       61 aatggggggt accagtatgt gcacaacggc ggtacggcgt ctggcactgt tgtaaacagt
      121 gacggctggc agattgtcaa aaacgggggt gtggccggga ataccaccgt taatcagaag
      181 ggcagactgc aggtggacgc cggtggtaca gccacgaatg tcaccctgaa gcagggcggc
      241 gcactggtta ccagtacggc tgcaaccgtt accggcataa accgcctggg agcattctct
      301 gttgtggagg gtaaagctga taatgtcgta ctggaaaatg gcggacgcct ggatgtgctg
      361 accggacaca cagccactaa tacccgcgtg gatgatggcg gaacgctgga tgtccgcaac
      421 ggtggcaccg ccaccaccgt atccatggga aatggcggtg tactgctggc cgattccggt
      481 gccgctgtca gtggtacccg gagcgacgga aaggcattca gtatcggagg cggtcaggcg
      541 gatgccctga tgctggaaaa aggcagttca ttcacgctga acgccggtga tacggccacg
      601 gataccacgg taaatggcgg actgttcacc gccaggggcg gcacactggc gggcaccacc
      661 acgctgaata acggcgccat acttaccctt tccgggaaga cggtgaacaa cgataccctg
      721 accatccgtg aaggcgatgc actcctgcag ggaggctctc tcaccggtaa cggcagcgtg
      781 gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcaccca gaaagccgtc
      841 aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga tgtcattgct
      901 cagcgcggta cagccctgaa gctgaccggc agcactgtgc tgaacggtgc cattgacccc
      961 acgaatgtca ctctcgcctc cggtgccacc tggaatatcc ccgataacgc cacggtgcag
     1021 tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccac ccgcacaggg
     1081 aagttcgtac cggcaaccct gaaagtgaaa aacctgaacg gacagaatgg caccatcagc
     1141 ctgcgtgtac g
//
LOCUS       DAYPHN010000350         1132 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000350 DAYPHN010000000
VERSION     DAYPHN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.359"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..504
                     /locus_tag="ACG0BW_004903"
     CDS             <1..504
                     /locus_tag="ACG0BW_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFK7261727.1"
                     /translation="DIVLESSSRISLKVSGSFVVIHSGGVDIVGPKINLNSGGSPGTP
                     VPALQPAVLKTLGDEKSGDGSDSGEENEDSGGNCVTGSGGDDRGDDEDEPEKYTLQFH
                     FTDDDGIPYSETRYIAFFEDGTQTRGETDEEGYTERFFVSSKHEIKVKLLFANDDFLS
                     MEGHYGR"
     gene            494..>1132
                     /locus_tag="ACG0BW_004904"
     CDS             494..>1132
                     /locus_tag="ACG0BW_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M35"
                     /protein_id="HFK7261728.1"
                     /translation="MDDNNQTSGQPKPEPEECVKEQKITDHFKIMIDKARKAQKLVLI
                     KRADDLLRWGAQEEYDFSKIFGVKGNKEVNIRKYGHNTGRRMNARFLMMDGVRRLMII
                     ANDLTMSSFINYTGCNEFAAFVSPSKDMPYIINIGAKFEYRDGKKNPVTGKDSHVATL
                     CHEMSHIQWYYGDNKKGGMWSQDYTTTDKYSTCKEDEVSYDEHIRIATKLISK"
BASE COUNT          375 a          170 c          299 g          288 t
ORIGIN      
        1 gatatcgtgc tggagagcag cagccggata agcctgaagg tgagtggctc gtttgtggtc
       61 atccattcag ggggcgtgga catcgtcggg ccgaaaatca atctcaatag cggcggtagt
      121 ccggggacgc cggtgccggc gctgcaaccg gcggtgctga agacactggg ggatgagaag
      181 agtggtgatg gcagtgattc cggagaagaa aatgaagact ctggagggaa ctgcgtgacc
      241 ggaagtggtg gtgatgatcg aggtgacgat gaggatgaac ctgaaaaata tactctccag
      301 ttccatttta cagacgatga cgggataccg tattcagaaa cccgctacat tgccttcttt
      361 gaggatggca cgcagacccg cggagaaacg gatgaagagg ggtatacaga gcgttttttt
      421 gtatctagca aacatgaaat taaagtgaag ttgttatttg cgaatgatga ttttctctca
      481 atggaaggac actatggacg ataataatca aacttctgga caaccaaagc cagagccaga
      541 agaatgtgtg aaagagcaaa aaataactga tcattttaaa ataatgattg ataaggctcg
      601 taaggcacaa aagttggttt tgataaaaag agcagatgat cttttgagat ggggagcaca
      661 ggaggaatat gatttcagta agatctttgg tgttaaagga aataaggagg ttaatataag
      721 aaaatatgga cataatacag gtaggcgcat gaatgctaga tttcttatga tggatggtgt
      781 gagaagatta atgattatag ctaatgattt aacaatgagc agttttataa attatacagg
      841 ttgtaatgaa tttgcagcat tcgtcagccc aagtaaggat atgccctata taattaacat
      901 aggcgcaaaa tttgagtata gagatggcaa aaaaaatcca gtaacgggaa aggattctca
      961 tgttgctact ttatgtcatg aaatgtctca tattcagtgg tattatgggg ataataaaaa
     1021 aggtggtatg tggtcacagg attatacaac tactgataaa tattcaacgt gcaaagagga
     1081 tgaagtttca tatgacgaac atatccgtat tgcaactaaa ctgatctcta aa
//
LOCUS       DAYPHN010000351         1083 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000351 DAYPHN010000000
VERSION     DAYPHN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.360"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..364
                     /locus_tag="ACG0BW_004905"
     CDS             <1..364
                     /locus_tag="ACG0BW_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261729.1"
                     /translation="HSVIKLSGISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEY
                     YSVQAVSMFTTNQFILLCPKDIIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPIS
                     AISVKRWDMPLQGSKPSN"
     gene            complement(590..765)
                     /locus_tag="ACG0BW_004906"
                     /pseudo
     CDS             complement(590..765)
                     /locus_tag="ACG0BW_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          352 a          210 c          213 g          308 t
ORIGIN      
        1 ccattcagtt attaagctct caggtataag tgacttcaaa atacatagct acattattaa
       61 aacaagtgaa tatccggagg agtttttttc caatgcagta tggggtaaaa aaaatataaa
      121 accgggggaa tattactctg tgcaggctgt ttctatgttt acgacaaacc agtttattct
      181 cctttgtccc aaggatatta taaggtttta tcgggaaagc tggaagttcg acctgcttaa
      241 tgtagacttc gatacaaata ccaggaagaa actccaggaa gaagcagcat attgcgtacc
      301 aatttctgca atatctgtaa aacgatggga tatgcctctt cagggctcga aaccctcaaa
      361 ttagaaggcc gctcgtgcga ctgtattaga tagtaatgcg gtatggaaaa atagtgatgg
      421 caagatgaac ttgacttggc taatcaaata ccggcgtagt gattaggaaa cagggctcgg
      481 ataacattat tgcgcggttc attgaaccta ctgatgacgc cggatatata gtcgtaaacc
      541 gtttcgaacc agtacttata tcaccaggag agtccatata tgaccaccgt ttacagtaaa
      601 aacggtatct gttttctgat ccctccagcg tcaaactacc gttgtaatgg tgaggtctgg
      661 tgctattgta ataattcaga atataatctc tgatttattt ccgaacctcg cccttgcctg
      721 cgtaaccatc cgtcgccccc atcctgtttt cagactgcgg aaaaaggcaa gtttttatcc
      781 ccccacagag attcaatcat atatcgcgtg ttttggaagt gacagttctt attcctgaga
      841 atactgcatg tgcaacacac tcggcatcca tcgataccac ttttatctcc aataggcagg
      901 ataaaaaaca agactgtgaa ttaaagtaat cggcaaatga atgagttaga agatactgaa
      961 aatgaatcaa ggactaaccc cgtagggaat gaaaataata aaagaacatc ttgtttttat
     1021 tttaatgaat gagcatgttc ttttatatta ataacaaaac ctatcattaa acaaaatgat
     1081 gaa
//
LOCUS       DAYPHN010000352         1043 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000352 DAYPHN010000000
VERSION     DAYPHN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.361"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..812)
                     /gene="wzx"
                     /locus_tag="ACG0BW_004907"
     CDS             complement(<1..812)
                     /gene="wzx"
                     /locus_tag="ACG0BW_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O15 family O-antigen flippase"
                     /protein_id="HFK7261730.1"
                     /translation="MSKTKLNVLYLAISQGANYLLPLLIFPYLVRVIGVSNFGDLSFS
                     LITIQVLLMVVEYGFGYSGTREIALNNDKKYHSEFFCGVVLARFILMLIAAIILIILC
                     FFYVFNDVKSLLCVGFLSVIAGVFNPNWFLQGKEMMSVMAVLSLFSRGIAVVAVYLII
                     KPATPMYISALLLSMPYILYSFCGVAYLLIIKEIFLCRPPIKKIQVILKNGFHFFCST
                     LATSAYTMLTPLVLGGVSGKFDVGIFNSANMIKQGLAGLASPLVQAFYPRIN"
     gene            complement(891..>1043)
                     /locus_tag="ACG0BW_004908"
     CDS             complement(891..>1043)
                     /locus_tag="ACG0BW_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFK7261731.1"
                     /translation="AADLFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVL
                     AGSGLS"
BASE COUNT          375 a          212 c          165 g          291 t
ORIGIN      
        1 atgttaattc ttggataaaa agcttggact aatggtgatg caagtccagc caaaccttgt
       61 ttgatcatgt tagctgagtt aaagatgcct acatcaaact ttccagatac gccacccaat
      121 acaagagggg tcaacattgt gtatgcacta gtcgcaagtg ttgaacaaaa aaaatgaaat
      181 ccatttttta aaattacttg aattttcttt atcggtggcc tacataaaaa aatctccttg
      241 ataataagta agtaggcaac gccacagaat gaatacaaaa tatatggcat gctcaataat
      301 aaggcactga tgtacatcgg cgttgcgggt tttataatta gataaactgc aacgactgct
      361 atgcctcgtg aaaatagtga cagcacagcc atcacactca tcatttcctt accttgcaaa
      421 aaccaatttg gattgaaaac acctgcaatt acggacagaa aaccaacaca taacaaagac
      481 ttaacgtcgt taaaaacata aaaaaaacag agtattatga gtattatagc tgcaattagc
      541 attaatataa aacgagcaag caccacaccg caaaaaaatt cagaatggta ttttttatcg
      601 ttatttagtg ctatttctct tgtcccacta tatccaaaac catattcaac aaccattaac
      661 aacacttgta tagttatcaa tgaaaaactc agatcaccaa aattcgatac accaatgact
      721 ctaacaagat aaggaaaaat taataatggc agtaggtaat tggcaccctg acttattgca
      781 aggtaaagaa catttagttt agttttgctc aatgaagtgc cccatataca acagatatca
      841 ttcgagtaac ttattcacat gaatcaataa gttgtttatt cagggcaaga ttacgaaagc
      901 ccgctccccg caagaactga cgccagatag tttctgtcca tggctgcttt tcgcatctta
      961 cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa tattaatagc aatgtgccgt
     1021 atccctgaaa ataaatctgc tgc
//
LOCUS       DAYPHN010000353         1041 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000353 DAYPHN010000000
VERSION     DAYPHN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.362"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>1041
                     /locus_tag="ACG0BW_004909"
     CDS             <1..>1041
                     /locus_tag="ACG0BW_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFK7261732.1"
                     /translation="VQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDC
                     GETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRA
                     ASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVREN
                     WQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVND
                     GFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNR
                     KLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQS
                     KTWVLFGKTNHGK"
BASE COUNT          268 a          248 c          298 g          227 t
ORIGIN      
        1 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
       61 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
      121 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
      181 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      241 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      301 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      361 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      421 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      481 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      541 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
      601 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
      661 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
      721 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
      781 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
      841 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
      901 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
      961 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1021 ggtaaaacca atcacggcaa g
//
LOCUS       DAYPHN010000354         1029 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000354 DAYPHN010000000
VERSION     DAYPHN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.363"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            45..830
                     /locus_tag="ACG0BW_004910"
     CDS             45..830
                     /locus_tag="ACG0BW_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261733.1"
                     /translation="MNSKKVCCICVLFSLLAGCASESPIDEKKKKAQVTQSNINKNTP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLSSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRQKTLTCNKDKNKDESLDIVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWTDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKLIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
BASE COUNT          330 a          177 c          220 g          302 t
ORIGIN      
        1 actaaatatt ctaatcatca tgacaggcga gggagtgtcc gattatgaat tcaaaaaagg
       61 tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tctcctattg
      121 atgaaaaaaa gaaaaaagct caagttacac aaagtaatat taataaaaac actcccccgc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggctgtatcc gaggaggata
      241 ttcaagctgc gcttgaagga gatgagtttc gtgtgcccct cagttctcct gtaatacttg
      301 ttcagtctgg tagtcgcgca ccggaaacca ttatgcagga ggagatgaga aaatattata
      361 cagttgccac attttctggc attcctgaca ggcaaaaaac tttaacctgt aataaagaca
      421 aaaacaaaga tgaaagccta gatattgtag atgccgaaaa tatgaactgg atgcaggcat
      481 tgcgttttgt tgcagcaaaa ggccatcaga aagcaattat tgttttccag gatacgttgc
      541 agacaggaaa atatgactct ggattaaaat caatagtttg gacagattat aaaaatcaaa
      601 aattgactga cacgatgtca ctgcgttatt tggttcgatt cacgctggtg gatgtggcta
      661 caggtgagtg ggctacctgg tcgcctgtga attatgaatc gaaactgatt tttccgcaga
      721 tagggaataa aaattctgac agccttgatg tggcagaaca gcagatttca caacttaagc
      781 agaaaacgta cgcagcagtg gttaaagacc tggtgaatcg atatcagtag tgaggtttta
      841 taccagacgt tttagtggta cttagcagga aagagcattt ttcttttctg ttttacacta
      901 tggaacaagt ctgctaatta attttacata tattttttag gagtagtaaa aattttcctc
      961 aagttatttg ttgtcctctc aacatataag ctgtagttcg agttttcggt tgaactgtca
     1021 aaaatacat
//
LOCUS       DAYPHN010000355         1022 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000355 DAYPHN010000000
VERSION     DAYPHN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.364"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            199..801
                     /gene="fimB"
                     /locus_tag="ACG0BW_004911"
     CDS             199..801
                     /gene="fimB"
                     /locus_tag="ACG0BW_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFK7261734.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          319 a          186 c          203 g          314 t
ORIGIN      
        1 gcaagagcat ggcgtttgta tggcaacgtt attataatta acagttgcta ctccatttaa
       61 gttcactcag aagaactggt ccacttacgt tagttattaa gcaaacgttc gcttttataa
      121 acataatcag gataaaaatg ttggattatt gctaacccag cacagctagt gcgcgtctgt
      181 aattataagg gaaaaacgat gaagaataag gctgataaca aaaaaaggaa cttcctgacc
      241 catagtgaaa tcgaatcact ccttaaagca gcaaataccg ggcctcatgc agcacgtaat
      301 tattgtctga ctttgctttg ttttattcat ggtttccggg cgagtgaaat ttgtcgattg
      361 aggatttcgg atattgatct taaggcaaag tgtatatata tccatcgatt aaaaaaaggc
      421 ttttcaacaa cgcacccgct attgaataaa gaagttcagg ctttaaaaaa ctggttgagt
      481 atccgtactt cgtacccgca tgctgagagc gagtgggtat ttttatcacg taaggggaat
      541 ccgctttctc ggcaacagtt ttaccatatt atctcgactt ccggtggtaa tgccgggttg
      601 tcactggaga ttcatccgca catgttacgc cattcgtgtg gttttgcttt ggcgaatatg
      661 ggaatagata cgcgacttat ccaggattat cttgggcatc gcaatattcg tcatactgtc
      721 tggtataccg ccagcaatgc agggcgtttt tacggcatct gggatagagc cagaggacga
      781 cagcgtcacg ctgttttata gagatagttg agataccagg gatggtgtta aaaactacat
      841 tattcttacg atatatcaaa aatgattaac catttattaa ttgatgttat tgtaagtgtg
      901 attcttaatt atcataataa acattaagtt aaccatatcc atacaaaata caatggttta
      961 tgttcttcaa aataaataaa caaaatcatt cataaattta cacatcactt aaaatctcct
     1021 gt
//
LOCUS       DAYPHN010000356         1005 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000356 DAYPHN010000000
VERSION     DAYPHN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.365"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..553)
                     /locus_tag="ACG0BW_004912"
     CDS             complement(<1..553)
                     /locus_tag="ACG0BW_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFK7261735.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCTWTYRGDECGY"
     gene            complement(563..892)
                     /locus_tag="ACG0BW_004913"
     CDS             complement(563..892)
                     /locus_tag="ACG0BW_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7261736.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
BASE COUNT          213 a          327 c          234 g          231 t
ORIGIN      
        1 gataaccgca ctcatcgccg cgataggtcc aggtgcaggt gttggccagc atgatacgtc
       61 ccggaaaaac agcgccgtcc gtttccgtcg gcgtggccag tacaaaagag gcactgaccg
      121 cgctcagttc gctgcactgc tcgatgcgcc agcggctgat cacctcctgc tccgggtcgg
      181 cgtcactgtt tccgttgacg aagttcaccg catccagaaa acgggcgtaa accttacgcc
      241 ggaccaccgt tccgccgacc agactctgca gatcttccgc catcccggtg accatgccgt
      301 gcaggttaga aaccgtcagt gtcggacggg cagcactgcc cttgccgttc agttcaaatc
      361 ctgtcccctg aatggggtat gcctgatact gccgcccctg ccaggtaacc ggctcacctt
      421 tttcgttctg ctcattacag aaaaaataac gttcaccacc gacctctgtc agatcgattt
      481 cccagagcac cacctgggct gactgagtga ggcgtgtcgt ctcatgatgt gtttcctgtg
      541 gaatatcctg catcagagcc tcctatgcca cgacctgttc aaaatctgcc gttatggtta
      601 cccacagcgc ccccacgctt gccgaccatt tacgacaaac caccctgatc ggtttccagt
      661 cataaggtgg cgtccactga aatgcacgga cgccaccgtg ccgttccaga aaggctttta
      721 aagatgggtg ttcacattta cgaacacgta tcgtcacgct gtaagtcgac aactggttat
      781 tcagtcccgc cgcacgacgc tgttcataac catcgcccag cttcactgtc accactttcg
      841 gctctgatac cacattcata tccgggcgca ctttccagtg aaacgtctcc attaccgata
      901 tgctccactt aaccgaccac catcacgggc ctgctgttgc ataaagtccg ctgccgcttt
      961 tttcccgagg tcataaaccg ccttcagggc agccggacct atctg
//
LOCUS       DAYPHN010000357          998 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000357 DAYPHN010000000
VERSION     DAYPHN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.366"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>998
                     /gene="espY4"
                     /locus_tag="ACG0BW_004914"
     CDS             <1..>998
                     /gene="espY4"
                     /locus_tag="ACG0BW_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HFK7261737.1"
                     /translation="CSFKNVVFSGVINSPNLEGPVFENCYFDDCKFNNIQLYETNDDT
                     VGSGNKKPIKGMFKGCFISKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQD
                     CIIQGGCCPGSSILLSHFYNCNIIGLDAHGMDFLANSFNKSNYDEIRGPDTGTVFYNC
                     NLKHIKINDGLREDGSDRSMFNPKEYFSDYIERRSRNLKLDDNFSKCVESIKKGTDSS
                     FSKKNTENNNVFFKNSNLIGANLGYYYSGDRCKDCAIDPNTNYSKDGLTAQKYMYMDL
                     DGAISKEQIPDFLKKVSAINIDVINFYNSESKTKEKYKNAFLELESFLSTLYVENKS"
BASE COUNT          356 a          124 c          192 g          326 t
ORIGIN      
        1 tgtagtttta aaaatgtagt tttttcagga gtgataaaca gccctaacct tgaaggtccg
       61 gtttttgaaa attgttattt cgatgattgt aaattcaaca acatacagct ttatgagact
      121 aatgatgata ctgtggggtc aggaaataaa aaaccaataa aaggtatgtt taaaggttgt
      181 tttatttcta aatgcaaaat tgaaaattat agatgtgaga cgtctaaaat ctacaccgtt
      241 acacaacctg atagtattca agaaaaatta ggttcttatc tctttatgca gtctttcgtt
      301 caggattgta taatccaggg aggatgttgt cctggctcga gtatacttct gagtcatttt
      361 tataactgta atataatagg gcttgatgct catggaatgg attttttggc aaattcgttc
      421 aacaagtcta attatgatga aatcaggggt ccggatacgg ggactgtgtt ttataattgt
      481 aatttgaagc atattaaaat aaacgatggt cttcgtgaag atggttcaga tagatctatg
      541 tttaacccca aagagtattt ttctgattac attgaaagaa gatcgagaaa tcttaagtta
      601 gatgataatt tttcgaaatg cgtagagagt ataaagaaag ggaccgattc ttcatttagc
      661 aagaaaaata cagagaacaa taatgtcttc tttaaaaata gtaatttgat tggggcaaat
      721 ttaggctatt attacagtgg agataggtgc aaagactgtg ctattgatcc aaatacaaat
      781 tattcaaaag atggtcttac agcgcaaaaa tacatgtata tggatctgga cggagctata
      841 agtaaagaac aaatacctga tttcttaaaa aaggtaagtg ctatcaatat cgatgtaata
      901 aatttttata attccgagtc aaagactaag gaaaaatata aaaacgcatt tttagagtta
      961 gagtcgtttt tatcaacact ttatgttgaa aataaatc
//
LOCUS       DAYPHN010000358          988 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000358 DAYPHN010000000
VERSION     DAYPHN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.367"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(106..960)
                     /gene="rclR"
                     /locus_tag="ACG0BW_004915"
     CDS             complement(106..960)
                     /gene="rclR"
                     /locus_tag="ACG0BW_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFK7261738.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          266 a          257 c          229 g          236 t
ORIGIN      
        1 aaaaaaatat ataaagccca aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac
       61 tcccggaatg cggattatct ttgctccgat tgctggcggt tttattcagg gtgcaagctg
      121 tctgaccctt tcccgatatt ctcccggagt acaaccaaac tcgcggacaa acgccttgtg
      181 aaaagatgat tcactggcat agcctactga ctcagcgatc accacaacag ggagcatttc
      241 ccgggaaaac atctgggccg ctatttgtag acgcaacttt gttaatacag ctagcggcgt
      301 ggttccggaa acatcacgga aaagctgggc aaaacttgcc cgggacatgt gggcgatgct
      361 ggccagcgat tcgacggtcc aggcgtgtcc tggcatttcc agcatttgct gtattaccgc
      421 accaagacgt ggatgcagaa gcaaactgag aatgtttttc tctgtattaa cctgtgcaat
      481 ccactcacgc accgcgaggg taaagaatgt agcgcagatc tggctacaca gagcatccac
      541 tcccggcatt gccgatctgg attcctgttg taaaaacggg atcgcctccc tcagccagtt
      601 atattccacg ctatggttca ctggtgctac aaacagcgtt tccggcaaag acgttaaaaa
      661 ataacgcgcc gaatgttgca gccgaagagt gccacagaca atacaggtcg attcattatc
      721 gacatggctc aggcgatgag ctgaattttg tggtagcagg accacatttc ccgggcgtaa
      781 tgtaaaaatc tctcccgtcg gcatttccag cttcgccgct ccttgcgtta acgcatgcca
      841 acgaataacc gacaattccc cggcaccatg tggaagttgc cagtcacttc ctaacacgca
      901 attcttatcg atcgttcctt gtggagcgtt aagcatcaac aaacggctaa gagcatccat
      961 atcagactcc tgagtagttt tttgatta
//
LOCUS       DAYPHN010000359          984 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000359 DAYPHN010000000
VERSION     DAYPHN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.368"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..856
                     /locus_tag="ACG0BW_004916"
     CDS             <1..856
                     /locus_tag="ACG0BW_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HFK7261739.1"
                     /translation="QPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNEMAELFD
                     IIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRNACLRFL
                     AFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWHQDGLVM
                     DKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFHAEDGSG
                     YQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLENLSGDLY
                     EKITKALA"
BASE COUNT          234 a          246 c          272 g          232 t
ORIGIN      
        1 tcagccgctg tctctgccgg tgcatgtggc tgatgctttc cgcgcggtac tgcttgatga
       61 gaagattgat ccggcactgg cggcagaaat cctgacgctg ccttccgtca atgaaatggc
      121 agaactgttc gatatcatcg acccgattgc tattgctgaa gtacgcgaag cactcactcg
      181 tactctggcg actgaactgg cggatgagct actggctatt tacaacgcga attaccagag
      241 cgagtaccgt gttgagcatg aagatattgc aaaacgcact ctgcgtaatg cctgcctgcg
      301 cttccttgct tttggtgaaa cgcatctggc tgacgtgctg gtgagcaagc aataccatga
      361 agcaaacaat atgaccgatg cgctggcagc gctttctgca gcggttgctg cacagctgcc
      421 ttgccgtgac gcgctgatgc aggagtacga cgacaagtgg catcaggatg gtctggtgat
      481 ggataaatgg tttatcctgc aagccaccag cccggcggcg aatgtgctgg agacggtgcg
      541 tggcctgttg cagcatcgct catttaccat gagcaacccg aaccgtattc gttcgttgat
      601 tggtgcgttt gcgggcagca acccggcagc gttccatgcc gaagatggca gcggttacca
      661 gttcctggtg gaaatgctta ccgacctcaa cagccgtaac ccgcaggtgg cttcacgcct
      721 gattgagccg ctgattcgcc tgaaacgtta cgatgctaaa cgtcaggaga aaatgcgcgc
      781 ggcgctggag cagttgaaag ggctggaaaa tctctctggc gatctgtacg agaagataac
      841 taaagcactg gcttgatact tttgagccaa aatgcctgat gcgacgcttg actgaactgc
      901 accccaaatg ttggacataa tctgacatct gaaggtgcag tttatgaaac attcatttga
      961 agtaaaactt gccgctgtaa atca
//
LOCUS       DAYPHN010000360          963 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000360 DAYPHN010000000
VERSION     DAYPHN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.369"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..242)
                     /locus_tag="ACG0BW_004917"
     CDS             complement(<1..242)
                     /locus_tag="ACG0BW_004917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261740.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..>963
                     /locus_tag="ACG0BW_004918"
     CDS             671..>963
                     /locus_tag="ACG0BW_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261741.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLG"
BASE COUNT          246 a          246 c          287 g          184 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcacta ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tat
//
LOCUS       DAYPHN010000361          956 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000361 DAYPHN010000000
VERSION     DAYPHN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.370"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>956
                     /locus_tag="ACG0BW_004919"
     CDS             <1..>956
                     /locus_tag="ACG0BW_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFK7261742.1"
                     /translation="KKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHY
                     FSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFA
                     GLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNND
                     FFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQV
                     AYGKSKFNASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEAV
                     AQYQFDFGLRPSVAYLQSKGKDLGVHGDRDLVKYVDVGATYY"
BASE COUNT          268 a          205 c          224 g          259 t
ORIGIN      
        1 gaaaaaatta acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc
       61 tcaggcagct gaaatttata ataaagacag taacaagctg gatctatacg ggaaagttaa
      121 tgccaagcac tacttctcct ctaatgatgc agatgatggt gatactactt atgcccgtct
      181 tggcttcaaa ggtgaaaccc aaatcaacga tcaactgact ggtttcggtc agtgggaata
      241 tgaattcaaa ggcaaccgtg ctgaatctca aggttcttcc aaagacaaaa cccgtcttgc
      301 atttgcaggc ctgaaatttg gtgattacgg ctccatcgat tacggccgta actacggtgt
      361 agcatacgac atcggtgcgt ggactgacgt tctgccagaa ttcggtggtg atacctggac
      421 ccaaacagat gtgttcatga ctggtcgcac tactggtgtt gcaacttatc gtaacaacga
      481 cttctttggt ctggtcgatg gcctgaactt tgctgctcag tatcagggta aaaatgaccg
      541 cactgacgta actgaagcca atggtgatgg tttcggtttc tccactactt atgagtatga
      601 aggattcggc gtgggtgcaa cctatgctaa atcagatcgc actgacggtc aggtcgccta
      661 tggtaagagc aaattcaatg cctccggcaa aaatgcggaa gtatgggctg caggcctgaa
      721 atatgatgcg aacaatatct atctggctac cacatattct gaaactcaga atatgaccgt
      781 ttttggtaat aaccatattg caaacaaagc acaaaacttt gaagcagtag cacaatatca
      841 gtttgacttc ggtctgcgcc catctgttgc ttaccttcag tcaaaaggta aagatcttgg
      901 tgttcatggt gaccgagact tagtcaagta tgtcgatgtc ggtgctactt actact
//
LOCUS       DAYPHN010000362          952 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000362 DAYPHN010000000
VERSION     DAYPHN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.371"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..302
                     /locus_tag="ACG0BW_004920"
     CDS             <1..302
                     /locus_tag="ACG0BW_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFK7261743.1"
                     /translation="RRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAA
                     LKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            308..898
                     /gene="caiE"
                     /locus_tag="ACG0BW_004921"
     CDS             308..898
                     /gene="caiE"
                     /locus_tag="ACG0BW_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFK7261744.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVMPKR"
BASE COUNT          210 a          228 c          312 g          202 t
ORIGIN      
        1 gcagacgaat gggcgcagaa gaggcgctgc gttggggggt agtcaaccgc gtggttagcc
       61 aggcggaact gatggataac gcccgcgaac tggcgcagca gctggttaac agcgctccgc
      121 tggcgattgc ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag
      181 cgtatcgcta tattcgcagc ggcgtgttga aacactatcc gtcggttctg cattcggaag
      241 atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt
      301 aacaaccgtg agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt
      361 tgtccatccc agtgcagtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg
      421 cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat
      481 tcaggatggc tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg
      541 ccatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg
      601 gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg ttgccgccat
      661 gagctttgtc aaagcgggct ttcgcggcga gaaacgccag ttgttgatgg gtacgccagc
      721 ccgcgctgta cgcagcgtga gtgacgacga gttacactgg aaacggttga ataccaaaga
      781 gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca
      841 aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgatgccga aacggtgatc
      901 tttccggatg cggcgtaaac gccttatccg acctggctgg aaagcaatcg ta
//
LOCUS       DAYPHN010000363          948 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000363 DAYPHN010000000
VERSION     DAYPHN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.372"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..948
                     /locus_tag="ACG0BW_004922"
                     /pseudo
     CDS             1..948
                     /locus_tag="ACG0BW_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    155..271
                     /locus_tag="ACG0BW_004922"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          223 a          233 c          294 g          198 t
ORIGIN      
        1 cgccggagac tctgcgtgtc tgggttcgcc agcatgagcg ggataccggg ggcggtgatg
       61 gagggctcac caccgctgaa cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac
      121 tgcgccgcag taacgatatc cttcgccagg cttccgctta ttttgcgaag gcggagttcg
      181 accgcctctg gaaaaaatga tgccactgct ggataagctg cgtgagcagt acggggtcgg
      241 accgctatgc agcgaactgc atattgcccc gtcaacgtat taccactgtc agcaacagcg
      301 acatcatccg gataaacgca gtgcccgtgc gcagcgcgat gactggctga agaaagagat
      361 acagcgcgta tacgatgaaa atcacaaggt atacggtgtg cgtaaagtct ggcgtcagtt
      421 gttacgggaa ggtatcagag tggccagatg cactgtggca cgtctcatgg cggttatggg
      481 acttgccggt gttctccggg gtaaaaaggt ccgtacgacc atcagccgga aagccgttgt
      541 cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc
      601 tgattttact tacgtcagca catggcgggg cttcgtctat gtggcgttca tcattgatgt
      661 gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct
      721 ggatgcactg gagcaggcgt tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga
      781 taaaggttct cagtatgtat cgctggccta cacacagcgg cttaaggaag ccggattact
      841 ggcatcaaca ggaagtacag gcgactcgta tgacaacgcg atggcggaga gcatcaatgg
      901 tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccgtgc
//
LOCUS       DAYPHN010000364          948 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000364 DAYPHN010000000
VERSION     DAYPHN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.373"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            411..>948
                     /locus_tag="ACG0BW_004923"
     CDS             411..>948
                     /locus_tag="ACG0BW_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261745.1"
                     /translation="MTIAAAWVRTLRNCKELIVVSDSRLNGGMKMDCGQKIITLPRSD
                     AFICFAGDTSWAYPLMHQVASAIDIYDRCSSRAQDIKELKTHILKIFERLREQIHDAI
                     GDMDIPDAKFIFGGYSWIRKKFMIWHITYQKSTNSFRADPAREIYGSPVVFTGDEEHV
                     IVANAMLREKLKSRGKLDN"
BASE COUNT          296 a          166 c          208 g          278 t
ORIGIN      
        1 ggtctttttt ggccgatgat acggtctttg ttccacggtg acgtgttcat actcaaatct
       61 cctgcaatgt gggagatttg agtatggttg tcccttatga gcgcaggctg tgtgaaaaca
      121 ctcaccaaat actaaaatgt atgaggttat gcaaaatctg gaatcaatca gctgattatc
      181 agagtcgaat tttacgtagg gaagatattt tcagctctgt tttaggccat ttctgcactc
      241 aaaaacgttt tcacacagcc gcggtcagat cgcaatcttg caccacaatc tggtacgagt
      301 caggcaagca tttggggtga aagcctgtcc tgagtgagaa tctaaccaag cattgtgtta
      361 tgtttaacaa ctggagtaga ctacaaaatc aattaattaa ggatcttgag atgactatag
      421 ccgcagcttg ggtaagaact ttaagaaatt gcaaagaatt gattgtagta tcagatagta
      481 gattaaatgg tggtatgaaa atggattgtg ggcagaagat aataactctg cctcgttcag
      541 atgctttcat atgtttcgca ggtgatacta gttgggcata tcctttgatg catcaagtgg
      601 catcagcaat tgatatatat gatcgttgct cctcacgagc ccaagatatt aaagaactca
      661 aaacccatat tcttaaaatt tttgaacgac tgagagagca aattcacgat gccataggtg
      721 acatggatat acccgatgca aaattcatat tcgggggata ttcatggata agaaaaaaat
      781 tcatgatttg gcacatcacc taccaaaaga gtaccaactc ttttcgtgcg gatcctgcac
      841 gtgaaatata tggatcacct gttgtattta ctggtgatga agaacatgta attgtagcta
      901 atgctatgct tcgtgaaaaa ttaaaatcaa gagggaagct ggataatt
//
LOCUS       DAYPHN010000365          918 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000365 DAYPHN010000000
VERSION     DAYPHN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.374"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            51..392
                     /locus_tag="ACG0BW_004924"
                     /pseudo
     CDS             51..392
                     /locus_tag="ACG0BW_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            389..472
                     /locus_tag="ACG0BW_004925"
     CDS             389..472
                     /locus_tag="ACG0BW_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261746.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(550..>918)
                     /gene="rdgC"
                     /locus_tag="ACG0BW_004926"
     CDS             complement(550..>918)
                     /gene="rdgC"
                     /locus_tag="ACG0BW_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFK7261747.1"
                     /translation="SGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEEITNHIEA
                     GKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFDADFILMT
                     GELAALIQNLIEGLGGEAQR"
BASE COUNT          241 a          226 c          220 g          231 t
ORIGIN      
        1 atatattcat aattcatcaa ggttgatata aatatcagcg aagatctcca gatattgttg
       61 cggaactggc gacgataaaa gataaatcag atgatgaatg gtggcgtgca ttgctgcaaa
      121 gtcgcaatga tcatttgcgc caaacggcat tgcgcaacgc acataccccg gcgtcgttgt
      181 taacggcatt gactgagcct caggatcggg cgttggttat taataatcca cagttggctg
      241 ccgatgtgaa aacgatgtgg ttaaaagatg atccatcatt actcttattt gtcgatcaac
      301 ccgctctttc gcagttacgg gatttagtga aaaccggggc aacgcggaaa attcgcagtg
      361 aagcgcgtca ccggcttgag gaaaaacaat gacacaacgc ccatggagca agttacagcg
      421 taaaacgcat aatattgctg cgctaaaaat tattgcccgc cgtagcgaat aattatatgc
      481 ctggtgtggc ttcgtacgcc ggataagacg cggcaggcgt cgcatccggc attaaaggta
      541 aatcagcaat taacgttgtg cttcgccacc taatccttca atcaggtttt gaattaacgc
      601 tgccagttca ccagtcatca ggatgaaatc ggcatcaaaa cgctgggcga aatcttcacg
      661 gtcgatatct tcgttttgat cgcgcagctc gtcgcagaac ttcagacgct tgagcgaacc
      721 atcgtcgcac atcacaaact gaatgcgctg ctgccagtcg agcgccagtt tagtcaccac
      781 ttttccggct tcaatgtgat tggtgatctc ttcgctggtc agatcttgtt tcttcgcgcg
      841 gatcactcca ccatcttcca gcagcgattt cagctcggct tcatcaagca gctggaagcc
      901 ctgtgccgcg ctgccgga
//
LOCUS       DAYPHN010000366          894 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000366 DAYPHN010000000
VERSION     DAYPHN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.375"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..894
                     /locus_tag="ACG0BW_004927"
     CDS             <1..894
                     /locus_tag="ACG0BW_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFK7261748.1"
                     /translation="LYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQR
                     LRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGIEQTINIVNKSELKKNFIIYGP
                     QLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSV
                     ICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFMN
                     RKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLQEIKLERPLPAVKLYISY
                     NKASLNNLVFSRFIDRLNDSF"
BASE COUNT          279 a          182 c          150 g          283 t
ORIGIN      
        1 ctttatgacc taaaaaaatt tgatctgaat ttactggtca tatttgagtg tatttatcaa
       61 catctcagca ttagtaaagc ggcagaatca ttatatataa cgccctctgc tgttagtcag
      121 tcgttacaac gtttacgtgc gcaatttaat gacccgttat tcattcgctc agggaaaggt
      181 atcgcgccga ctacgacagg gttaaatctg catcatcatc ttgagaaaaa tctcagaggc
      241 attgaacaaa cgatcaatat tgttaataag tctgagctaa agaaaaattt tattatctac
      301 ggtcctcagc ttatttcttg tactaataac aacatgctaa tccgctgtct tagacaagat
      361 acttcaatcg atatagaatg ccatgacata ctgctttcag ctgaaaatgc cgaagaactg
      421 ttggttcaac gcaaaactga cctggcaatt acactacaac cagtaattag ccgctctgtc
      481 atctgcatgc cgttacatac tattcgcaat tctcttatat gtagcaacaa acatcctcgt
      541 attaccgata ccagtaacta tgaacaaatt atcgccgaag aattcacctt gttgatatct
      601 aaatcagcag ggattgacga aattcaaatg gacatagatg aacgttttat gaaccgtaaa
      661 gtttcatttc gcagttcttc tttgttgacg ataattaata gtatttctgt tactgactta
      721 ttgggcattg tgcccaccga actgtacgat ttacatcgtg attttttgaa gttacaagag
      781 ataaaactgg aacgaccact tcctgcggtt aaactttata tttcctataa caaggcttcg
      841 ttgaacaacc ttgtcttctc acgattcata gatcgtttaa atgatagctt ctga
//
LOCUS       DAYPHN010000367          889 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000367 DAYPHN010000000
VERSION     DAYPHN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.376"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..577)
                     /locus_tag="ACG0BW_004928"
     CDS             complement(<1..577)
                     /locus_tag="ACG0BW_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFK7261749.1"
                     /translation="MDNLIGTPPNYVVPHKYIDTEQMEYLCHLNRFSKSPDDFLITGQ
                     REDLFTYKYILDGSFSNLQHLFPKGQLQKIQQRLSSLMYKNMFHCFHVFLLKLCSIES
                     IPLPNADYAFFDDEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSADHHHNFS
                     TTIALTNDSFHISSINNHNAQVIFDENIHLHP"
BASE COUNT          299 a          133 c          164 g          293 t
ORIGIN      
        1 agggatgtaa atggatgttt tcatcgaata taacctgtgc gttatggttg tttattgaag
       61 atatatggaa gctatcattt gttaaagcta tggttgtgct aaaattatgg tggtgatcag
      121 cgctcatgaa atcaaatgta acaatgtcgt tgtttccttt cttttctttg tgatatgcgt
      181 ttaaaaaaga aatattttcg atctgttcat cagttaatgt aaatggcatt tcgtcatcaa
      241 agaaagcgta atcggcattt gggagaggaa tactctcaat tgagcatagt tttaataaaa
      301 aaacatgaaa acaatgaaac atgtttttat acataagaga acttaatcgc tgttgtatct
      361 tctgtagttg tcctttggga aataaatgct gtaaatttga aaatgagcca tctaaaatat
      421 atttatatgt aaaaaggtcc tctctctgac cagttatcaa aaaatcgtca ggtgatttag
      481 aaaaacgatt gagatgacat aaatattcca tttgctccgt gtctatatac ttatggggaa
      541 ctacgtagtt tggtggtgtg ccaataagat tatccataaa tcaccttgat aaaagttaat
      601 gaaatctatt tattttaacc tcgacaataa aagcggctat aaaaaaacgc tcattttctg
      661 atgctgcggt taacccgtgg aatgtaccgt taacccccta aaaattgcaa atccagggtg
      721 aacaccctgg gttaaaatgg aaataatgag ctatttttta cagtattcac cggtaatata
      781 agtaacactt gttgctaacc taactagcag tacagttaat taacaacata gaagttattg
      841 tttttcaaac aaaacaactc aatattgcac gccggagggt ttatggttg
//
LOCUS       DAYPHN010000368          889 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000368 DAYPHN010000000
VERSION     DAYPHN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.377"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(543..749)
                     /locus_tag="ACG0BW_004929"
     CDS             complement(543..749)
                     /locus_tag="ACG0BW_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFK7261750.1"
                     /translation="MELYREYPAWLIFLRRTYAVAVGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          259 a          196 c          178 g          256 t
ORIGIN      
        1 ttctatgatt ttattcatgt taagaatacc tgagcggtta attatcggtt attgtgatga
       61 atatatattc gcttttaatt ttcaataact catcacggaa atacatattg ctttgtaggc
      121 aagcgctaat aatactttat gaacctgact tccgccagat attcaaccta aatattcatg
      181 acgcagtaat gttttagaaa gccactaact gcccagatag tgcatcatct actacattgg
      241 cgattatact ctaaataatt caagttgcag gaaggcgaca agcgagtgac aaattcgtca
      301 ggaacgaatt tgcccagtcg caggctgacc ttcggtgaga gacatggatg tctctcatta
      361 atccccagga gcttacataa gtaagtgact ggggtgaacg aacgcagtcg cagcacatgc
      421 aacttgaagt atgacgagta tataaaaaac ccctgattcc agttccttgt gctgcgatcc
      481 taaccaaaaa aaaaccgcca gcgtttccgc tggcggtttt caatgtattc agaactcagc
      541 aatcagtaaa aatcaccggt tgccttttcg gcctgatcca tccaaaccgg tttatcgctg
      601 gttttcgacc agacgcgatg cagatagctg taaaagcggg cgcggtcttt ccagaaaagc
      661 atgaaaggca gcgccagaac gcccactgca accgcataag tacggcgtaa aaagataagc
      721 catgcaggat attctctgta gagttccata cttttctccc ccttttgtat ctacccggtg
      781 aatggcaccg gaaaaatgaa tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat
      841 tttactactc atccgaccac ttatttttgc ttattgatgg tttatttgc
//
LOCUS       DAYPHN010000369          854 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000369 DAYPHN010000000
VERSION     DAYPHN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.378"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            181..852
                     /locus_tag="ACG0BW_004930"
     CDS             181..852
                     /locus_tag="ACG0BW_004930"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFK7261751.1"
                     /translation="MDKVLDSAILSSANKRKGILAIDAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
BASE COUNT          248 a          190 c          174 g          242 t
ORIGIN      
        1 acttaacata tgaactacat aaaagattac gcagtcgtat ccccgtacct gaaagcccct
       61 tatttttcag ctatcagtaa tttttaacac ttaaaaatta gcatctgtct gtgccagaga
      121 ttacgtctct gcgggcatcg ttgtgcttgt agtataataa ataaaatgga gtgtcattaa
      181 gtggataagg ttttagattc agccatcctt tcttcggcaa ataaaagaaa gggtatcctt
      241 gctattgacg cacatcctga cgatatagaa ttgggctgtg gcgcgtcgct tgctcgtctt
      301 gcgcaaaaag gaatttatat cgcagccgtg gtgatgacta ccggcaactc tggcacagat
      361 ggaataatcg atcgccatga agaatcgcgc aacgccctaa agatattagg gtgccaccaa
      421 actattcatc ttaattttgc tgacacccgc gcgcatttgc agctcaatga tatgatttcc
      481 gccctggaag acatcattaa aaatcaaatt ccttctgatg ttgaaatcat gcgggtatat
      541 accatgcatg atgccgaccg ccatcaggat catcttgctg tttatcaagc ttcaatggtt
      601 gcctgccgaa ctattccaca aattctcggc tacgaaaccc caagcacctg gctttcattt
      661 atgcctcagg tttttgaatc cgttaaagaa gaatatttca cagtcaaact tgctgcatta
      721 aaaaaacata aaagccagga acgacgcgat tatatgcgtc atgatcgcct gcgtgcagtt
      781 gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg aaggctttgt aattcataaa
      841 atgattcttt gagc
//
LOCUS       DAYPHN010000370          851 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000370 DAYPHN010000000
VERSION     DAYPHN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.379"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..633
                     /gene="rfaY"
                     /locus_tag="ACG0BW_004931"
     CDS             <1..633
                     /gene="rfaY"
                     /locus_tag="ACG0BW_004931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFK7261752.1"
                     /translation="NDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYAPKHKMI
                     ERFLKSCVKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEG
                     VGLNEYLEISEDLKEQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTA
                     VLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKAIRDVKVKLGLKSK"
     gene            658..>851
                     /locus_tag="ACG0BW_004932"
     CDS             658..>851
                     /locus_tag="ACG0BW_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFK7261753.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIA"
BASE COUNT          315 a          118 c          154 g          264 t
ORIGIN      
        1 aatgactttt taacttgcag ggttaaaaca ttaaaagttt ttcgttctat tgatgatact
       61 aaagtcattc ttattgacac tgctcgtgga cctcttgtac ttaaagttta tgcgccaaag
      121 cataaaatga tcgaacgttt cttaaaatct tgcgtaaaaa aagattatta tgaaaattta
      181 atttatcaaa ccgatcgggt acgtggtgaa ggcattcaat caattaatga ctattttttg
      241 cttgctgaac gtaaaacatt aaatttcgcg cattattata ttatgctcat tgagtatatt
      301 gaaggtgtcg ggttaaatga gtatttggaa atttctgaag atctgaagga acaattatca
      361 gaatcgataa aagagttgca tcagcatgga atggtgtctg gcgatcctca taaaggaaat
      421 tttatagtat ccgaaaaagg tttaagacta attgaccttt caggtaaaaa aacaaccgca
      481 gttttaaaag caaaagatcg cattgatctt gaacggcact ataatataaa aaatgaattg
      541 aaagactttg gctatacata tctcatattc aaaaagaaaa ttaaaaaagc aatcagggat
      601 gtaaaagtaa aactaggatt gaagagcaaa taatgattat gatgaagggt aattaaattg
      661 gattttaaac atcttactca atttaaagat ataattgaac tggacaagcg ccccgttaaa
      721 cttgatgaac gggaaacgtt taatgtctca tggggtattg atgagaacta ccaggttggg
      781 gctgcgattt caattgcttc aattcttgaa aataataaac aaaacaaatt tacctttcac
      841 ataatcgctg a
//
LOCUS       DAYPHN010000371          831 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000371 DAYPHN010000000
VERSION     DAYPHN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.380"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            11..373
                     /gene="yibL"
                     /locus_tag="ACG0BW_004933"
     CDS             11..373
                     /gene="yibL"
                     /locus_tag="ACG0BW_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFK7261754.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          272 a          192 c          160 g          207 t
ORIGIN      
        1 aagtcatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtcttagcg atcgcctgga
       61 cgctatccgc catcagcaag ccgatctgtc actggttgaa gccgcagaca aatatgccga
      121 gctggaaaaa gaaaaagcca ctctggaagc agaaattgct cgcctgcgcg aagttcacag
      181 ccagaaactc agtaaggaag cacagaagct gatgaagatg ccgttccagc gcgcgattac
      241 taaaaaagag caagctgata tgggcaaact gaagaagagc gttcgcggac tggtcgttgt
      301 gcacccaatg accgcgctgg gccgcgaaat gggcctacaa gagatgacag ggttctctaa
      361 gactgcgttt taagttcctt tctcgtcgct gtaattacag ccactcacga tttactcgct
      421 cctgctggca catgcaggag cctgtttcag agatcgaata tttataacgt tcttaacatt
      481 atatattcaa cgccacctac acttctgagc gcccggagca ggctaaattc tgaaaatcag
      541 gtacaaatta aagaatgatg tatcaatatt aacaatgaat tgccgcatat caatgcttga
      601 aacataatta attatttaaa tttttctatc agcatcgcaa aattcgcaat aaaacatcac
      661 tcaactgaat atttatcttt ccctgccccg ggcctttatc cggtgaaaca tttgacattg
      721 attgacaccg atcattaaaa tccgcgcgat caatttattt attggctaca aactaaaatt
      781 gtttgacact taaaaacata ttcgcacaac ccattaatac aaaaattctc t
//
LOCUS       DAYPHN010000372          818 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000372 DAYPHN010000000
VERSION     DAYPHN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.381"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            278..769
                     /locus_tag="ACG0BW_004934"
     CDS             278..769
                     /locus_tag="ACG0BW_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261755.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKYVNPVKITMRSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFPITDDLMSMATNELIKLA
                     KIENNLDLNKFSTICTDVLSPLVTRHNKEKKPDDLLPFAKIPFLIFIEKMEIDNMNQG
                     HAY"
BASE COUNT          330 a          119 c          136 g          233 t
ORIGIN      
        1 gaaaataata aaggaaatct tttgagatgt ttgtcacgaa taaagctatt tatgttacca
       61 atatcatttc gagtgtgcac actatctatc tgttttttca ggacataaaa aacaacatgt
      121 aagttatgta aattgccgat gctactaggt aacattatgg taaaaaaagt aaaaatgagc
      181 gatttttgat agaataaagc atgagaaaag gtgatatttg acattatcag aagctgcgaa
      241 ttcggatttt gctctaatca aatgagagaa atgtaaaatg aagccacgaa atattaataa
      301 tagcctacca ctgcaaccat tagttcctga tcaggagaac aaaaataaga aaaatgaaga
      361 gaaatacgtg aatccagtta aaattacgat gaggtctggt ttaaattata ttgaacaaga
      421 atctcttgga ggaaaatatc taacacatga tttgtcaata aagatagcgg atatttctga
      481 agaaataatt cagcaagcaa tattatcggc tatgagcata tataaatttc cgataacaga
      541 tgatttaatg agtatggcta caaatgaact cataaaactg gccaaaatag agaataattt
      601 agacctgaat aaattctcta ctatatgcac cgacgttcta tcccccctcg tcaccagaca
      661 taataaagaa aaaaaaccag acgatttact acccttcgca aaaatcccct ttttaatttt
      721 catcgaaaaa atggaaattg ataatatgaa tcaaggtcat gcatattaag gcatttatga
      781 aatgtcgtaa ataagatgac taataaggtg aatattag
//
LOCUS       DAYPHN010000373          799 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000373 DAYPHN010000000
VERSION     DAYPHN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.382"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            18..275
                     /gene="pemI"
                     /locus_tag="ACG0BW_004935"
     CDS             18..275
                     /gene="pemI"
                     /locus_tag="ACG0BW_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFK7261756.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            277..609
                     /gene="pemK-mt"
                     /locus_tag="ACG0BW_004936"
     CDS             277..609
                     /gene="pemK-mt"
                     /locus_tag="ACG0BW_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFK7261757.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
BASE COUNT          180 a          184 c          231 g          204 t
ORIGIN      
        1 ttttatggag gtgaagaatg cataccaccc gactgaagag ggttggcggc tcagttatgc
       61 tgaccgtccc accggcactg ctgaatgcgc tgtctctggg cacagataat gaagttggca
      121 tggtcattga taatggccgg ctgattgttg agccgtacag acgcccgcaa tattcactgg
      181 ctgagctact ggcacagtgt gatccgaatg ctgaaatatc agctgaagaa cgagaatggc
      241 tggatgcacc ggcgactggt caggaggaaa tctgacatgg aaagagggga aatctggctt
      301 gtctcgcttg atcctaccgc aggtcatgag cagcagggaa cgcggccggt gctgattgtc
      361 acaccggcgg cctttaatcg cgtgacccgc ctgcctgttg ttgtgcccgt aaccagcgga
      421 ggcaattttg cccgcactgc cggctttgcg gtgtcgttgg atggtgttgg catacgtacc
      481 acaggtgttg tacgttgcga tcaaccccgg acaattgata tgaaagcacg gggcggaaaa
      541 cgactcgaac gggttccgga gactatcatg aacgaagttc ttggccgcct gtccactatt
      601 ctgacttgaa catggggttt gaggggcaac tggatgaaaa cgtacgattt agggcactaa
      661 aaccgctgtt gtcccaccat tctggtgatt cccaaacgtt atttggctaa aaagtagttt
      721 tgatgtggtt tattttccga attccaagcg cagccctact ttcttgtgcg tatttgcgtg
      781 ttttgcgcag ttttgaagt
//
LOCUS       DAYPHN010000374          797 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000374 DAYPHN010000000
VERSION     DAYPHN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.383"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            173..>797
                     /locus_tag="ACG0BW_004937"
     CDS             173..>797
                     /locus_tag="ACG0BW_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261758.1"
                     /translation="MANIIISKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKSGKCVYSIADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGAGSAKAGTQQYRFIKNDFYN"
BASE COUNT          276 a          151 c          140 g          230 t
ORIGIN      
        1 atttatttag ggaacacctt cgacgatctc gcttttattt ctaggacttt catccctgca
       61 aaaaatttaa aaaaaacact tgcaatcttt tttcgcgtat cgataattga actcgtcgaa
      121 agcgaagacg ctaacgacaa taaattttaa atttacataa ggaaaaatta tcatggctaa
      181 cattatcatt tctaaaaaat ccatcattga agctgcatct attgtatccg atgaactgcg
      241 cgaaaaagca gatctggcaa ctcaaacata taacgaacat tataaaaatg gtacgcacac
      301 taaagcagac aaagcaaata tgcaagctgc gaccactaaa cttgcttact tcatcaacaa
      361 cgtcgtaaac gcagtagaag acgaaaaatt atgctctgtt ttctactatg cgattaaagc
      421 aagcaaacaa gcgccagaag tatttttccg tgatgcaatg actaatagtt attctctgga
      481 aaaactggtt tatctggtta aatcaattaa atctggtaaa tgcgtttatt ccatcgctga
      541 tatgtcagga tctcgtgtat tcgctttaat cgatatgatt aacgacgaga tcgacacgtt
      601 caccaatggc gctgttttcg atttaatgaa tgaagctaaa aaagcgtgtg aaattaaatt
      661 ggacgctggc tatactcaag ccaaccagtt gatcaatctt tgcgaacgtc ttggacttgt
      721 tgaaaaagtc aaaggagctg ggagcgcgaa agctggtact cagcaatatc gctttattaa
      781 aaatgatttc tacaatt
//
LOCUS       DAYPHN010000375          792 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000375 DAYPHN010000000
VERSION     DAYPHN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.384"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(98..253)
                     /gene="hokD"
                     /locus_tag="ACG0BW_004938"
     CDS             complement(98..253)
                     /gene="hokD"
                     /locus_tag="ACG0BW_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFK7261759.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          211 a          201 c          173 g          207 t
ORIGIN      
        1 cagataaaat aaacccgccg aagcgggtta agtgcaggtg cattgaggat gcctgacaca
       61 tcagaggtgg cgagggattt ctccctcgcc tggtctctta ctcctcaggt tcgtaagctg
      121 tgaagacagc gacctccgtc tggccggttc ggattcgtac ctcgcagagg tctttcctcg
      181 ttaccagtgc cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct
      241 tttgctgctt catagcctgc ttctccttgc ctttcggcac gtaagaggct aacctagatt
      301 tgccgttcat agattgagcc tcagattaat gttaaacgtc ttgcaggacg cgtaatgtta
      361 actggggctt ttctctatct gcctttggtg ttcatgcctg aggcagatag cctcaagcac
      421 ccgcagcaat tctacttaac tcccgtcacc tcgccaatat aaaatcaatc agaaaggcga
      481 tccgtaagaa caacagcaag acaataaatt gccattacag ccgcaatagc tagcgcacat
      541 ttgagaacca gcacgacaac ctcctgtatt ggacgtacac cagtcctgat aaatatgagg
      601 ctgtctcatc attgattcaa tataactatt gggtatagtt tctgtgattt tgttctgtag
      661 aaatggaaca caacaaccag tcaccaccag cacttcttta aatacgccaa gtccgacgca
      721 agctaacctt ctagtccgct ttgggcgaag accggacgtt cgataggtgt gaactagtaa
      781 ctttaaaaac at
//
LOCUS       DAYPHN010000376          781 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000376 DAYPHN010000000
VERSION     DAYPHN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.385"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..752
                     /locus_tag="ACG0BW_004939"
     CDS             <1..752
                     /locus_tag="ACG0BW_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFK7261760.1"
                     /translation="NTFNEKIRFMLIDSGKKHLDRNRIIEIDKVTFSENHIFSTSIHA
                     KAQLNAKNKEKLLRSYSREDVFNYLETINEIYGLRNTPDSLNQLCIVTYSWLMGNTLN
                     LLISNAIKYSNSVRDPISYRWVKFDKTNPDHINAKIMEAIQCIESEVTFKLETCIAHF
                     YQLCQSIHGDENAGINLSPYLEYGTLDTNIIELQEFGFSRLAAIEIIAKHKECVTFKT
                     NETSLQINTQKLRAKIEKHSVIDRELSWLNL"
BASE COUNT          301 a          119 c          121 g          240 t
ORIGIN      
        1 taaatacctt taatgaaaag attcggttta tgcttattga ttcaggaaaa aaacatcttg
       61 atcgtaatag aattattgaa atagataaag tgaccttttc agaaaatcat atattctcaa
      121 catccataca cgctaaagcc caactcaatg ctaaaaataa agaaaaatta ttgcgttctt
      181 atagcagaga ggatgttttc aattatttgg aaacaataaa tgaaatatat ggcctgcgta
      241 acacccctga ttcattaaac caactgtgca ttgtgactta ttcgtggctc atgggtaata
      301 cgctgaattt attaatatca aacgctatta aatatagcaa ttcagttaga gatcctatta
      361 gctatagatg ggttaaattt gataaaacca atcctgatca tataaacgca aaaataatgg
      421 aggcaataca atgcattgaa tctgaagtta cttttaaact tgaaacatgt attgctcatt
      481 tttatcagct atgtcaatca attcacggcg atgaaaatgc aggcattaat ctttcacctt
      541 atttagaata tggaacatta gatactaaca tcatagaact tcaagagttt ggtttctctc
      601 gtttagccgc aattgaaatt atagcaaagc acaaagagtg tgttacattc aaaacaaatg
      661 agacatcatt gcaaataaac actcagaaat taagagctaa gattgaaaag cacagtgtaa
      721 ttgatagaga actatcatgg ttgaatttat aattcagcca tgataaataa taatttgaaa
      781 g
//
LOCUS       DAYPHN010000377          780 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000377 DAYPHN010000000
VERSION     DAYPHN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.386"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(12..608)
                     /gene="fimE"
                     /locus_tag="ACG0BW_004940"
     CDS             complement(12..608)
                     /gene="fimE"
                     /locus_tag="ACG0BW_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFK7261761.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          244 a          189 c          146 g          201 t
ORIGIN      
        1 aataagttaa atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc
       61 cataatccgg caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt
      121 cgatgcccga gataatcctg aattaaacga gtatctgcac cacgctccgc caattcataa
      181 ccgcaagcat gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg
      241 gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata
      301 aatatagcgt cagtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc
      361 acggcttcac gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg
      421 cgaatattta ttctaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca
      481 ctaatacgca tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt
      541 gccccgtaac aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt
      601 ttactcacaa tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc
      661 tcaatcggtc aaaattagcc caatagataa aaagataacc acacacggag tgatgtggct
      721 atcagtcaat tacattaaat atcatacaaa aataaaatat cactgatgtg acaacatcta
//
LOCUS       DAYPHN010000378          763 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000378 DAYPHN010000000
VERSION     DAYPHN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.387"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..87
                     /locus_tag="ACG0BW_004941"
     CDS             <1..87
                     /locus_tag="ACG0BW_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HFK7261762.1"
                     /translation="VTYDFERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(190..513)
                     /gene="iraM"
                     /locus_tag="ACG0BW_004942"
     CDS             complement(190..513)
                     /gene="iraM"
                     /locus_tag="ACG0BW_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFK7261763.1"
                     /translation="MKWIVIDTVIQPTCGISFSAIWGDMKMIIWYQPTIFLTPGSIFT
                     PVKSGIIFKDKEYPITIYNITPFNKDLWSLLKSSQECPPGESEITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          256 a          113 c          124 g          270 t
ORIGIN      
        1 gtaacctatg acttcgaacg tctgatggaa ggcgctaaat tgctgaaatg tagcgagttt
       61 ggtgatgcga tcatcaagaa catgtaatct ctacatgtgt taaatattga aacgggcgta
      121 taacacgccc gttgttttat ttatgtggat attattaata tcatgtcgag aatatttata
      181 tgaagaccat tacttgagtc catatgggca tatttttata atacaactat tatgtaaaca
      241 tttatttgtt atttcgcttt ctcctggagg acactcttga ctgcttttga gtaaactcca
      301 taaatccttg ttgaatggtg tgatgttata aatagtaata gggtattctt tatccttaaa
      361 gataatacca gacttaaccg gtgtaaatat acttccagga gtgagaaata tagtaggttg
      421 ataccagatg atcattttca tatcacccca tatggctgaa aaagatatac cgcatgtagg
      481 ttgaattacc gtgtcaatta ctatccactt catttgttat gtcttatccc acggtattta
      541 atatggttca ttaggatgtt tatttcttga ctttgcatat gtgtatatta ctccccaaaa
      601 aacaaattaa tcaaaaatta aaaccattgt ttatataaaa caaaatttaa agcaagaaat
      661 ctcaatagat attaaacaaa atgagattta gtgaaaatat tagattattc acttcgtttt
      721 agatttgttt agctaaaatg ttatacattc aaatgactga aca
//
LOCUS       DAYPHN010000379          760 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000379 DAYPHN010000000
VERSION     DAYPHN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.388"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..101)
                     /locus_tag="ACG0BW_004943"
     CDS             complement(<1..101)
                     /locus_tag="ACG0BW_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261764.1"
                     /translation="MILWSMKKETDIRRGRHCVFLMHVHLVFVTKYRR"
     gene            109..>760
                     /locus_tag="ACG0BW_004944"
     CDS             109..>760
                     /locus_tag="ACG0BW_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFK7261765.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQ"
BASE COUNT          210 a          179 c          192 g          179 t
ORIGIN      
        1 cgtcggtatt tggtgacaaa gaccaggtga acatgcatca ggaaaacaca atgccttcca
       61 cgccggatat cggtttcttt tttcatagac caaagtatca tttgacctgt gaaacgatta
      121 caggcattta aattccagtt aagaccaggt ggtcaacagg agtgtgaaat gaggcgcttc
      181 gcaggcgcat gtcgtttcgt tttcaatcgc gctctggcgc ttcagaatga gaatcatgag
      241 gccggtaata aatacatccc ttacgggaaa atggcttcct ggctggttga gtggaaaaac
      301 gccactgaaa cgcaatggct taaagattcg ccctcacagc cattgcaaca gtcactgaaa
      361 gaccttgagc gggcttacaa aaacttcttc cagaatcggg ctgcttttcc ccgattcaaa
      421 aagcggggac agaatgatgc attccgctat ccgcagggcg ttaagctcga tcaggaaaac
      481 agccgtattt ttctgccgaa actgggctgg atgcgctacc ggaacagccg tcaggtcacg
      541 ggtgttgtaa aaaatgtcac tgtcagccag tcctgcggta agtggtacat cagtattcag
      601 acagaaagtg aagtatcaac tccggttcac ccttcagcat caatggtcgg gctggatgct
      661 ggcgtggcta aactcgccac gctgtcagat ggcacagtct ttgagcctgt aaacagtttt
      721 cagaaaaacc agaagaagct ggcgagactt cagcgacagt
//
LOCUS       DAYPHN010000380          753 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000380 DAYPHN010000000
VERSION     DAYPHN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.389"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..179)
                     /locus_tag="ACG0BW_004945"
     CDS             complement(<1..179)
                     /locus_tag="ACG0BW_004945"
                     /inference="COORDINATES: protein motif:HMM:NF037477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HFK7261766.1"
                     /translation="MKNKETLKQIYHLQYDSFAFKSGLINWYNSLLDKTPDELDENDI
                     AKMLRQNILPALAVQ"
     gene            complement(192..476)
                     /locus_tag="ACG0BW_004946"
     CDS             complement(192..476)
                     /locus_tag="ACG0BW_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261767.1"
                     /translation="MEIKMQDVSVILKLIARGLIDIRTAANSGNAKACFILSDFIHVL
                     PHTANCMVNDGRRYEDVVHDLYERAKIKNMDDWLENALNDIELNQKNHSK"
     gene            complement(559..>753)
                     /locus_tag="ACG0BW_004947"
     CDS             complement(559..>753)
                     /locus_tag="ACG0BW_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFK7261768.1"
                     /translation="DLIERNETYDIQRVEPDYFLIGQDGDLGYFIYVGSAKESDTIFS
                     IDLGALGAMEFSVEADDVYF"
BASE COUNT          255 a          148 c           90 g          260 t
ORIGIN      
        1 ttttgtacag caagcgctgg taagatattc tgacgaagca ttttggcaat atcattttca
       61 tccagttcat ccggtgtttt atccaataaa gagttatacc agtttattaa accacttttg
      121 aaagcgaatg aatcatattg caggtgataa atctgtttta gggtttcttt atttttcatt
      181 ttataatgtc tttatttgct atgatttttc tgatttaatt ctatatcatt taaagcattt
      241 tcaagccaat catccatgtt cttaatttta gcccgctcat acaaatcatg cacaacatct
      301 tcataacgcc gtccatcatt gaccatacaa ttagctgtat gaggcagaac atgtataaaa
      361 tcagacaata taaaacaagc ctttgcattt ccagagttag ctgcagttct gatatcaatc
      421 aagcctctgg caattaattt aagaataaca ctaacatctt gcattttaat ttccatatca
      481 tccagttaaa ttttagtcgt aaatcgtcta tctaaaaaag atgaaattaa catttataaa
      541 tttattacaa tataaaaatc aaaaatatac atcatcagct tctactgaaa actccatggc
      601 tcctaaggca cctaaatcaa tactaaatat ggtatcactt tccttagcac tcccaacata
      661 gataaaatag cctaaatccc catcctgacc tatcaaaaaa tagtcaggct ccactcgttg
      721 aatatcatat gtttcatttc tttcaataag atc
//
LOCUS       DAYPHN010000381          743 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000381 DAYPHN010000000
VERSION     DAYPHN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.390"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..429)
                     /gene="mepH"
                     /locus_tag="ACG0BW_004948"
     CDS             complement(<1..429)
                     /gene="mepH"
                     /locus_tag="ACG0BW_004948"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFK7261769.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTIKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQK"
BASE COUNT          188 a          136 c          185 g          234 t
ORIGIN      
        1 tttttgcacc ttcgctttgt gcgcgtcggc aagagttcct gacgcggcat ttttgacttt
       61 cacacaatgc gatttatagc ccttacgttt ggtacacttt tcggtaaagc tggctgaggc
      121 cgttttattg acggcgtgtt tgctgcgagt tttggcgttt ttagaagcgg taatggaaga
      181 ttttttaacg gaagaagcgg ttttgctttt gatcgtactt ttactggcgg cttttttcgc
      241 tgttttctgg ctggctttct gggtcttttt ggtggttgcc gtgcttttct ttttatctgc
      301 ttttttggtg atatgagtag tagcagaact ctccctggct tgctttgaag catgggccat
      361 aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa tacggtttat
      421 ccgcgccact gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc tgatttacaa
      481 aacttgatga ttctaatgca tagaagaaga gaaagttaat agcttttatt gttaaaaaat
      541 acgtttcgtc ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg tcataaatac
      601 gccagtttgc cttgcgtcag ggaagaaacg aatatttgaa atgcaacaaa tagagcaaag
      661 ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact gacccgctac
      721 aatgtcagat tactgccgta aag
//
LOCUS       DAYPHN010000382          739 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000382 DAYPHN010000000
VERSION     DAYPHN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.391"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..69
                     /locus_tag="ACG0BW_004949"
     CDS             <1..69
                     /locus_tag="ACG0BW_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261770.1"
                     /translation="KMRKAAMDRDYLASVLAGSGLS"
     gene            178..738
                     /locus_tag="ACG0BW_004950"
     CDS             178..738
                     /locus_tag="ACG0BW_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261771.1"
                     /translation="MKKSKKIKTMNHQNNSGIQNQPDYHIEIERKNMVRLLDERIDKV
                     YGSLLGPMKKKNIDKIRHFTLDVMVFILDTNPRVCNKLSREIISLAQNTFIEKNVNFS
                     TFNNEQHRIREYMNEGGGIRWFTWDNPEISFVRASFGLFVKMETKGDHEHTLSDTFLQ
                     IVECVNLNIGLIAKDELVKIINKHFM"
BASE COUNT          276 a          114 c          140 g          209 t
ORIGIN      
        1 aagatgcgaa aagcagctat ggacagagac tacctcgcgt cagtccttgc ggggagcggg
       61 ctttcgtaat cttgccctgg cgatcgaata gcgcagatga cggtgctcta aatttgaatc
      121 taataagccg gtgttatttt tcatccacta ttaaaatctt gacacaacga ggcaacaatg
      181 aaaaaaagca aaaaaattaa aacaatgaac catcaaaata atagcgggat acaaaatcaa
      241 ccagactatc acatcgaaat agaaagaaaa aatatggtaa gattgcttga tgaacgtatt
      301 gataaagttt acggttcatt gctaggacca atgaaaaaga aaaatataga taaaatacgt
      361 cattttacac ttgatgtaat ggtgtttatt cttgacacga accctcgtgt ttgtaataaa
      421 ttatccaggg aaataatatc tttagcgcaa aataccttca ttgaaaaaaa cgttaatttt
      481 tcaactttta ataatgaaca gcatagaata agggagtata tgaatgaagg tggtggaata
      541 cgatggttta catgggataa ccccgagata tcttttgttc gtgcatcttt tggccttttt
      601 gtcaaaatgg aaacaaaagg tgatcatgaa catacactaa gtgacacatt tcttcagatt
      661 gttgagtgtg ttaatttaaa tataggactt attgcaaaag atgaactggt aaaaatcatc
      721 aataaacact ttatgtaaa
//
LOCUS       DAYPHN010000383          734 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000383 DAYPHN010000000
VERSION     DAYPHN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.392"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>734
                     /locus_tag="ACG0BW_004951"
     CDS             <1..>734
                     /locus_tag="ACG0BW_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7261772.1"
                     /translation="AGSATAASQSKTAAESAATRAEAAADRAEEIAGAVAMEDASLTT
                     KGVVKLSSAVDSTSESLAATPKAVKTAYDNAEKRLQKDQNGADIPDKGRFLNNINVYS
                     KGEVDQKKGMRYVVVNAPAGVQEGKYYPLVIKRNDSHRASRVVISTPSRSANHRMNNC
                     EFNGFVCAGGWTDRGSYACGMFWAYSSSERAIHSILMSNKGDTVDSVFYIEGGAFPVE
                     VFLEEGLSVTAPASDYIVAETTYKFG"
BASE COUNT          199 a          154 c          198 g          183 t
ORIGIN      
        1 ggcaggcagt gcgacggcag catctcagag caaaactgct gctgaatccg ctgcgacccg
       61 tgcggaagct gctgctgatc gtgctgaaga gattgccggt gcagttgcga tggaagatgc
      121 aagccttacc accaaaggtg ttgtgaaact tagcagtgct gtcgacagca ccagtgaatc
      181 tttagccgcg acaccaaaag cagttaagac cgcatatgat aatgctgaaa aacgactaca
      241 gaaggatcag aatggtgctg atatccccga caagggccgc ttcctgaata acattaatgt
      301 ttacagcaaa ggtgaagtgg atcagaaaaa ggggatgcga tacgttgtgg tcaatgcccc
      361 ggcgggtgtc caggaaggta aatattatcc gttagtgata aaaaggaatg acagccatcg
      421 ggcatcccgc gttgtcattt caacgccaag tcgctcagct aatcacagaa tgaataattg
      481 tgagtttaat ggttttgttt gcgcaggcgg ctggacggac cggggtagct atgcctgtgg
      541 tatgttctgg gcttattcat catcagaacg cgctattcat tccattctga tgagtaataa
      601 aggcgatacc gtagacagcg tattctatat agaaggtggg gcatttcccg tagaggtctt
      661 tttagaagaa ggtctctccg ttactgcacc tgcttctgat tatattgttg ctgaaacgac
      721 ttataaattt ggcg
//
LOCUS       DAYPHN010000384          719 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000384 DAYPHN010000000
VERSION     DAYPHN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.393"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            38..>719
                     /locus_tag="ACG0BW_004952"
     CDS             38..>719
                     /locus_tag="ACG0BW_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFK7261773.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPLSQSVNITPASPSVLVGKTVQLSAGISMGKSASSFTWKSANDQIATVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPDSTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATAMVEVTAPV
                     VAVTGVEID"
BASE COUNT          205 a          166 c          181 g          167 t
ORIGIN      
        1 gtgggctata cgcccaccct ttttgtagga gcgagaaatg ccagaacaaa agatgaagat
       61 tacggaagag gcattttcgg attttacggg gcatatgtgc cgtgccggat ttaccaattc
      121 tatctccgat gaacctttaa ccgagcggca acaaagtcag ctatctgctt gtttgcaggc
      181 ggttcccctc tcccaatccg taaacataac cccggcttca ccgtcagttt tggttgggaa
      241 aactgttcaa cttagtgcag gtattagtat gggtaagagt gccagttcat tcacctggaa
      301 gtcagccaat gaccaaattg caaccgttaa cggcactgga ttagtaactg gcgtagcccc
      361 aggcaaagta aaaattactg caactgatca ggaaacccag ctttccgctt cagtggaagt
      421 caccgtaaat ccggtagccg ttcaatccgt aacggtaacg ccagactcaa cctctgttga
      481 gaaaggaaaa tcagtcagtc tgaaagcgaa tgttcagccg tcaaatgcga cgaataaggc
      541 tgttacctgg tcttccaaaa atgaagataa ggcaacggta gaccagagcg ggaacgtaac
      601 tggtgtagaa gttggcactg cgacgattga gatcgtttcc caagatggta gcaaaaaggc
      661 aactgcaatg gtggaagtaa ctgcgcctgt tgtggctgtt accggcgtcg aaatcgacc
//
LOCUS       DAYPHN010000385          708 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000385 DAYPHN010000000
VERSION     DAYPHN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.394"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..598)
                     /locus_tag="ACG0BW_004953"
     CDS             complement(<1..598)
                     /locus_tag="ACG0BW_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261774.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVM
                     NIKSILVLVCCTTILSLIMIIFIFFITSFFIPIVIPYDIKNLPAYNKIQGNFLSVMML
                     SGLFPMAFIYVLYCAFDFDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKN
                     NRMKLLRRVQIYLIIPLSIALLVHLQVIPLEMVFRNIET"
BASE COUNT          273 a          122 c          105 g          208 t
ORIGIN      
        1 aagtttcaat attcctaaaa accatctcca gtggaattac ctgaagatgc accagtaaag
       61 caatagacaa cggaatgata agatagatct gaacacgtct caacagtttc attctattat
      121 ttttaaacga taagtcatat tcaagatacc tcgtgtttat tatggctgaa ataacaacta
      181 ttagtactcc tatacttaaa atagataacc agccagagtt atctttaaca ctctggtcaa
      241 aatcaaatgc acagtataac acgtatataa aggccatggg gaagaggcca gataacatca
      301 ttacagataa aaaattaccc tgaatcttat tgtaagcagg aagatttttt atatcataag
      361 gaataactat tggtataaaa aaagatgtta taaaaaaaat aaaaatgatc ataatcaatg
      421 atagaatagt tgtacaacaa accaatacca agatgctttt aatattcatc acatcaaaga
      481 aaatatcaag tcgatccaga cgactgaaat atccccagag aactatcaac gcaatccctg
      541 atataatact tgatggtgtt aatactgaaa ctattagcgt cttccacttc tgattcattt
      601 taacttcctg gttttgctat aaagagagca taaaaaagag tgaataagtt attacacttc
      661 attgtttgac tcctcgcagt aacctttcag gaaggttgtc ggatattt
//
LOCUS       DAYPHN010000386          703 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000386 DAYPHN010000000
VERSION     DAYPHN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.395"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>703
                     /locus_tag="ACG0BW_004954"
     CDS             <1..>703
                     /locus_tag="ACG0BW_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFK7261775.1"
                     /translation="NIQALRFIAAAMVVLAHAELGQYGIGNIEILASLGGFGVIIFFI
                     ISGFIIPYVAYGGSQSEGEFKISAGNFFMRRVIRIIPVYALITALCVLSAFIVKHYIS
                     SPTPPIAYWWPETKISLQWYLESITFTHWHRDAILSIGWTLQLEFLFYTSFAFFIAIK
                     LSRLEYIEILFLTISIVANILTYQNNSIIFEILPFMKTLARPEMVVFAMGMFMYRLYL
                     LGAMLKKTIALTILSL"
BASE COUNT          212 a          120 c          123 g          248 t
ORIGIN      
        1 caatatacaa gcactaaggt ttattgctgc agctatggtg gttttggcac atgccgaact
       61 tggacaatat ggcattggga atatcgaaat ccttgcatct ctgggaggat ttggcgttat
      121 tatatttttc ataattagtg ggtttattat accttacgta gcatatggtg gctctcaatc
      181 agaaggagaa ttcaaaatat ctgcagggaa cttttttatg cgaagagtaa taagaataat
      241 cccggtatat gctttaatta ccgccctttg cgttctatca gccttcatag taaaacatta
      301 tatatcttcc ccaactccac ctattgctta ttggtggccc gaaacaaaaa tcagtcttca
      361 atggtatctt gagagtatta cattcactca ctggcatcgc gatgcaattc taagtatagg
      421 ttggacatta caacttgaat ttttgtttta tacttctttt gcatttttta ttgctatcaa
      481 attatcaagg ttagagtata tagagatctt atttttaact atatccatag tagctaatat
      541 tttaacttat caaaataaca gcatcatatt cgaaattctt ccgttcatga aaacactggc
      601 aagaccagaa atggtcgtat ttgctatggg tatgtttatg tatcgattat acttactggg
      661 cgctatgtta aaaaaaacaa ttgcgcttac aatattaagt ttg
//
LOCUS       DAYPHN010000387          696 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000387 DAYPHN010000000
VERSION     DAYPHN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.396"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..105
                     /locus_tag="ACG0BW_004955"
     CDS             <1..105
                     /locus_tag="ACG0BW_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFK7261776.1"
                     /translation="QHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            120..338
                     /locus_tag="ACG0BW_004956"
                     /pseudo
     CDS             120..338
                     /locus_tag="ACG0BW_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(388..>696)
                     /locus_tag="ACG0BW_004957"
     CDS             complement(388..>696)
                     /locus_tag="ACG0BW_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFK7261777.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT          180 a          177 c          158 g          181 t
ORIGIN      
        1 caacatctgg caaggctggg acggaagtca ctgtcgttct caaaatcggt ggagctgcat
       61 gacaaggtca tcgggcatta tctgaacata aaacactatc agtaagttgg agtcattacc
      121 ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgca ttacttgact
      181 gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac
      241 ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg
      301 ctgtataccg ttggcatgct agctttatct gttcgggcac tgttgcaaag ttagcgatga
      361 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
      421 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
      481 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
      541 gttgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
      601 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
      661 accataggcg ggcgctttat ccgtgttgat gaatcg
//
LOCUS       DAYPHN010000388          693 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000388 DAYPHN010000000
VERSION     DAYPHN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.397"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..234
                     /locus_tag="ACG0BW_004958"
     CDS             <1..234
                     /locus_tag="ACG0BW_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261778.1"
                     /translation="VVKGVIQCIGYIDKNNKASCDEFYSGSYECLDDDQETLNYLNES
                     CAEGIDADKDLIRKNELLALSLKELYQLLVSKK"
BASE COUNT          245 a          110 c          147 g          191 t
ORIGIN      
        1 gttgttaaag gcgtgattca atgcattggt tacattgaca aaaataacaa agcaagttgc
       61 gatgaatttt attcaggcag ctatgaatgc ttggatgatg atcaggaaac actgaactat
      121 ttaaatgaat catgcgcaga gggtatagac gctgataaag atctcatcag gaagaatgag
      181 ttattagcct tatccctgaa ggaattgtat caacttctcg tttcaaaaaa ataacaagta
      241 atacggcgag cgatatgacg tgccatggga tatccgcacc cagcagcatg ttgtctccac
      301 gatgtccgca gttcgctcat aatagacatt cactacagtt atagtagaag gtatgcatgc
      361 tgctgagtgg ggaaagtgtg aaagaaaaga agacagctgc tttgtttgtc gtcacgttta
      421 tcttcattgg ctatgcaagt cgtaatacaa ggcgggacaa aactgagaca cataaggcct
      481 cgcaatggct tgcaaggcca cacatgtatt gagatgttaa taaaatgtag acttgtaatt
      541 ttgatataaa tggtagagaa aatctttccc caaaataaaa acgaacgtca atgaaatcaa
      601 acggttgaat aaagttgatt ttggctaata caaagacaag aaaataatat ttatgattaa
      661 atatcagcga gttgaataca taatttttat ata
//
LOCUS       DAYPHN010000389          693 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000389 DAYPHN010000000
VERSION     DAYPHN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.398"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>693)
                     /locus_tag="ACG0BW_004959"
     CDS             complement(<1..>693)
                     /locus_tag="ACG0BW_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFK7261779.1"
                     /translation="YNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYNKHEK
                     ILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNA
                     GFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISGKYNTR
                     YSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWCKEDLL
                     KPVNSNQYRYCA"
BASE COUNT          220 a          133 c          106 g          234 t
ORIGIN      
        1 ttgcacaata ccgatactga ttgctattca caggtttaag taaatcttct ttacaccacg
       61 gagaagctgc tttggcgatc aaaaaactac gtgacaccgg ataatcagcc cactcatgcc
      121 agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact ggattatcaa
      181 ctttgtcttt taattcatag ttaatactat atcgggtatt atatttcccc gaaataaact
      241 tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg attttactta
      301 cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt agattccact
      361 catcaatatt tatcagcaaa aaaccagcat tgaaatatcc agaagctaat tgtggtgtag
      421 ttaataccga ggctcgattc tgccaccatt caacatctct ttcagctaca actgcggcaa
      481 tttcattagt agagaattga taatctaaga gttctttaat actacccttg caagcaatat
      541 ctgcatcaag gtatagtatt ttttcatgtt tattatagaa ataatctgca attataaatc
      601 gaaaatatgt agcgtaagtc cagtttttcg tgcttggtaa tgacttcagc ttatcacaat
      661 tgataacata aatattaatt cttgaattat att
//
LOCUS       DAYPHN010000390          680 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000390 DAYPHN010000000
VERSION     DAYPHN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.399"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..186)
                     /locus_tag="ACG0BW_004960"
     CDS             complement(<1..186)
                     /locus_tag="ACG0BW_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7261780.1"
                     /translation="MTKNTRFSLEVRQRAVRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGD"
BASE COUNT          196 a          155 c          150 g          179 t
ORIGIN      
        1 atcaccgccc ccggtatccc gctcatgctg gcgaacccag acacgcagag tctctggcgt
       61 acagccaatc tttggagcaa tggaacaaat tgccgcccat tgtgagtcat attcgtcctg
      121 actttccaga accatacgaa ctgcccgttg acggacttcg agggaaaaac gtgtattttt
      181 agtcatcctg tttacctctt tctcagggaa tttagtctcc aggattcccg gggcggttca
      241 ggatgatgag tttctggtac gtcacattta cgacaactac tgcattgtca gggaaaaagg
      301 tgtgaatgtt cctgtattga aaaattttgt tcagatatgt attcagctat ggtaaccagt
      361 acccacaatt ttgtagctca ccagttgatg aacttaagaa agggctggat gcactccggg
      421 attatcctgt acacaaattg cgctttcaac gatttgttaa accgatggta tttggtaata
      481 cacagattaa ctgggaagaa gcctattcca gtttcagaca aacagcgcta agtgttctga
      541 ccagctaaca aacgcgttaa gtacgattcc acaattcagc atatcgaacc agaccaaatg
      601 attacagcaa agcacaaaaa agagggatta tagcgtcaaa agaattcgaa ttgcacatgg
      661 gggcctcatt ggtggcctca
//
LOCUS       DAYPHN010000391          677 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000391 DAYPHN010000000
VERSION     DAYPHN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.400"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>677
                     /locus_tag="ACG0BW_004961"
     CDS             <1..>677
                     /locus_tag="ACG0BW_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261781.1"
                     /translation="DREVCPELRVKLLDNLNYSRNTLDNLRGDLNKPTITSSQSSSST
                     EQTTIKISKFNILDMLDNLTMRQQAVMEEFLRVFTSMYKASAMSEAIMSNVFEQLVQY
                     QRKETERATAASSIGVIIGSGVGAVGASASAVANYRSHPKKMVTLTPDQEHTIERTDD
                     NAQRASKREVIEQQASLKQIDEKIKNTTKDTNLNEKEKEEALKSYDKEKEATLEKIRD
                     TNEKIST"
BASE COUNT          268 a          121 c          135 g          153 t
ORIGIN      
        1 agacagagaa gtctgtccgg aactaagagt aaaactattg gataatctta attattcaag
       61 gaatacgcta gataatttga ggggtgacct taacaaacct acaattacat ccagtcaatc
      121 aagcagttct acagagcaga ccactattaa aatatctaaa tttaatatcc ttgatatgct
      181 agataatttg accatgcgac aacaagctgt tatggaggaa tttttaaggg tattcacatc
      241 aatgtataaa gcctcagcta tgtctgaggc tattatgagc aatgtttttg aacaacttgt
      301 ccagtatcag agaaaagaaa ccgaacgcgc cacagcagca agttcgatag gtgttattat
      361 cgggtcagga gttggcgcag tcggggcctc ggcatctgcc gtagcaaatt acagaagcca
      421 tcccaaaaag atggtaaccc ttacacctga ccaagagcat actatcgaaa gaactgatga
      481 taatgcacag cgcgcgtcaa aaagggaagt tatagagcaa caagcttctc tcaaacaaat
      541 agatgaaaaa ataaaaaata caacgaaaga tacgaatctt aatgaaaaag aaaaagaaga
      601 ggcgctgaaa tcatatgata aagaaaaaga agctactcta gaaaagatta gagatacaaa
      661 cgagaaaatt agcacaa
//
LOCUS       DAYPHN010000392          671 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000392 DAYPHN010000000
VERSION     DAYPHN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.401"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..607
                     /locus_tag="ACG0BW_004962"
                     /pseudo
     CDS             1..607
                     /locus_tag="ACG0BW_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT          219 a          127 c          165 g          160 t
ORIGIN      
        1 ctgcaaccta ttgatggcgt ggcggtaagt tatattgatg ctgccgtggc gttgggtaat
       61 accattaacg aaatggataa atattacacc caggaaaatt ataaagacga tgcgtttgcg
      121 aaagggaaaa cgctgcacca gacattctta aaaaatcttg aagcctttga acctgtagca
      181 gaatcttatc atgcggcgat tcaggaaatt aacgataagc gccagcttgc ggaactgaaa
      241 aatattgaac aaagagaagg gaaaacattc cactactact ctctggcagt catgatttca
      301 gcgaaacaaa ttaataacct gatttcgcaa gagaagtttg atgttgatgc ggcaatgaag
      361 aaagtctctg aactggaaac gctggtggcg caggccaaag aagcggataa aggcggtatg
      421 aatttctcgt tcattaattc ggcagaccag tatcaactcg aggctaaaaa atatgttcgc
      481 cgcatcagag ataaagtcct gtactctgac tgggataaag agcaacttca ggacgcaaac
      541 tcaagctgga tggccgaaga ctcatttccc gagaacatta tgcgagtaca acgaaatggt
      601 cgatgaaata tttcagtcaa ttgtaatcgc ggggcgggtt tgtgatgagc gcggatatgt
      661 cacgaagtcg t
//
LOCUS       DAYPHN010000393          663 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000393 DAYPHN010000000
VERSION     DAYPHN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.402"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            89..580
                     /locus_tag="ACG0BW_004963"
     CDS             89..580
                     /locus_tag="ACG0BW_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFK7261782.1"
                     /translation="MNTDTLEIFNNSDEWASQLKHALSQGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDESVKSKAQAYIELLTDNSE
                     DNG"
BASE COUNT          258 a          106 c          104 g          195 t
ORIGIN      
        1 actcataatt caaaaaagat aaatgcaaaa aaataatgag tcaattaata ataatttagc
       61 atcacgaaac acatcacaga ggaatattat gaacacagat acacttgaaa tattcaataa
      121 cagtgatgaa tgggcaagtc aactaaaaca cgcattatca caaggagaaa atctggcatt
      181 actacatgga ttgactcctg atatccttga tagaatatat gcatatgcat tcgactacca
      241 tgaaaaaggt aatataacgg acgcagaaat ttattataaa tttctgtgca tttatgcgtt
      301 cgaaaatcat gaatatctaa aaggttttgc atcagtatgc cagtcaaaaa agaaatatca
      361 acaagcatat gacctttaca aactaagtta caattactcc ccgtatgatg actattcagt
      421 tatttatcgt atgggtcaat gtcaaattgg ggctaaaaat atcgataacg caatgcaatg
      481 tttctatcat attattaaca attgtgagga tgaaagtgtt aagagcaaag cgcaggcata
      541 tattgaactc ttaaccgata attcagaaga taatggctaa gcattacaat catttggtta
      601 aaattgctgg ttatagtgca gttaaccact atgaccatct tctcttatgt tttcttaaat
      661 caa
//
LOCUS       DAYPHN010000394          648 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000394 DAYPHN010000000
VERSION     DAYPHN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.403"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            232..402
                     /locus_tag="ACG0BW_004964"
                     /pseudo
     CDS             232..402
                     /locus_tag="ACG0BW_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          196 a          156 c          132 g          164 t
ORIGIN      
        1 tcattccgat tccgggcacc actaattctt aagaacccgc ccacaaggcg ggtttttgct
       61 tttggatctt tatataaaat aaacttgggt gcataacgcc atcaggtctt aaacactccc
      121 gaatcatcaa taaacatccc ccgctaacaa aacacagttc agttcacata acatccctgt
      181 tatcccacac atatctgctg gccgaacgac gccagtgcgc gtacggaggc aagagcagcc
      241 ctgcacatgg cagcgcttgt ggtaacctgg tttaatccgg tgaaagaggc gttttacatg
      301 catctgccag cagccggaaa aaccaaaaaa gtggcgctgg ttgactgtat gcgtaaattg
      361 ctgaccatcc tgaacgcaat gctcagaaag aacgaagagt gaaatgaatc gtagcatcat
      421 gttgctccat aatttttacg ttcaagacag ttgattatcg gcgcatatga ctcaaaaaga
      481 gcctgatgat tgcctctttt ccataggctc tttctctgaa gtgaaggaag gcccggaaga
      541 aagcactcgc tggcatacgt gactccagct caaccggaga taatcactat tgttattttt
      601 ctatttaata ataatctcct gaaaaataga tatggcaaaa atacaatc
//
LOCUS       DAYPHN010000395          633 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000395 DAYPHN010000000
VERSION     DAYPHN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.404"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>633)
                     /locus_tag="ACG0BW_004965"
     CDS             complement(<1..>633)
                     /locus_tag="ACG0BW_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFK7261783.1"
                     /translation="FTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVD
                     GFSIIPENFFTLLSGNFKADWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPK
                     NPNTAYFGDTDGRVGAVLYALLVSGHIGIRGEGWSLLCQLLKHEDMASFAYENKKLKK
                     LFTLLDTRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYIL"
BASE COUNT          214 a          126 c           97 g          196 t
ORIGIN      
        1 taaggatata tttatcacca aaaatagtac taaatctatc gcctgtatga tccccaagga
       61 agatacaagg ggtaatcgca tcgcctttta aaaaaacatg ttgatgcaac tcattcaaga
      121 tcatgtctct cgtatctaat aaagtgaaga gttttttcaa ttttttattt tcatatgcga
      181 atgacgccat gtcttcgtgt tttaataatt gacataataa actccacccc tctcccctga
      241 tgcctatatg ccctgaaacg agcaatgcat aaagtacagc gcccactctt ccatctgtat
      301 cgccaaaata agccgtgttg ggattttttg ggttaatcac accacactgt ttataaaagt
      361 ctatttttga aaaaacagat tcaacatagc tatcaaactt ttccttagta aacgtctctt
      421 tagagacagc ccaacgccag tcagctttaa aattaccgct aagaagagta aaaaaatttt
      481 caggtatgat agaaaaccca tcaactttat aaattatgtc tgatttatcc ccggagaaat
      541 taataaaata ataaccgtcc ttacgctcaa tcgtaacggt aatgggagac gataatttta
      601 tcacatggtt attgtctggt ataattgtaa aga
//
LOCUS       DAYPHN010000396          632 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000396 DAYPHN010000000
VERSION     DAYPHN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.405"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..278)
                     /locus_tag="ACG0BW_004966"
     CDS             complement(<1..278)
                     /locus_tag="ACG0BW_004966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261784.1"
                     /translation="MKDFTLKFRDREGYKAFLNEINWEDNEVLQDQLLIDEIGFAFDE
                     VGRNADDEPIYQRRDGYFVNIRILDEKLDASPFTGNVVILDAPLREWA"
     gene            complement(275..>632)
                     /locus_tag="ACG0BW_004967"
     CDS             complement(275..>632)
                     /locus_tag="ACG0BW_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFK7261785.1"
                     /translation="ATNDEQLVLAFRTPTSPDVEPLPYVQFGVRSAYLSPSVKAGRKM
                     IAGFVCNSSTEFRCCCNGYLSPVQTSSWKNTGAAHEIRIGGTSAGGERHLFGHVRNLR
                     IWHKELTDAQMGESVE"
BASE COUNT          184 a          160 c          109 g          179 t
ORIGIN      
        1 caagcccatt cacgaagagg agcatcaaga atcactacat ttccggtaaa tggtgatgcg
       61 tccagctttt catctaagat tctaatattt acaaaataac cgtcgcgtcg ttgataaatt
      121 ggttcgtcgt cggcatttct gccgacttca tcgaaagcga atccaatttc atcaatcagg
      181 agttgatctt gcaatacttc attatcttcc caattaattt cattcaagaa tgctttatag
      241 ccttcccgat ctcgaaactt cagcgtaaaa tctttcattc tactgactcc cccatttgcg
      301 catcagttaa ttctttatgc catattctca ggttacgaac gtgaccaaac aagtgacgtt
      361 ctcctccagc agaagttccc ccaatacgta tttcatgtgc agctccggta ttcttccatg
      421 acgaagtctg aacaggtgac aggtaaccat tacaacagca acgaaattca gtcgagctat
      481 tacagacaaa accagcaatc attttgcgcc cggccttcac agatggacta agatatgcag
      541 atctcactcc aaactgaaca taaggtaaag gttctacatc cggacttgta ggcgtcctga
      601 aagccaatac cagttgttca tcattagtcg cc
//
LOCUS       DAYPHN010000397          622 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000397 DAYPHN010000000
VERSION     DAYPHN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.406"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..518)
                     /locus_tag="ACG0BW_004968"
     CDS             complement(<1..518)
                     /locus_tag="ACG0BW_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFK7261786.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQL"
BASE COUNT          171 a          141 c          133 g          177 t
ORIGIN      
        1 agctgattct taattgccgc agtcgtggca tcattaaaaa tgaatttata cctcacaggt
       61 tcttccgcac tgtttttctg aagtacttcc agttgtctga gggtttctga ctcatccgcg
      121 cctttgcgga gttctttatt cagttttgac aacaatgctt ttttctgagc ctttaaaagc
      181 tcttccttgt ttttccagac agccagttca cttttgtatc tggaatataa ctgcatctcc
      241 tgctggtaca ggggcttcat taataatttg tccactgcgg tttttcgttc tccggactcg
      301 gcaagcgtca ggaaaaaaag agagaccggc ccccgcagtg tattcagtct gcagacatca
      361 atcagattct ggcagacaag tgaaacagcg cccagggcag acgctgccac aagcccaagg
      421 ggagcctgcg taatttgctg agcttcaata acggcatcac gaatgagtgg aggcagcgaa
      481 ctcaggggaa actcatcaca aggctgaccg aatgacatag taacctcact atacacagat
      541 ataaaagcac tgcaattctg gctattaata gcaaaaatag cagtcaaatc aatatgaggt
      601 aaaatttttt gttgatttaa tg
//
LOCUS       DAYPHN010000398          617 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000398 DAYPHN010000000
VERSION     DAYPHN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.407"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..555)
                     /gene="espX1"
                     /locus_tag="ACG0BW_004969"
     CDS             complement(<1..555)
                     /gene="espX1"
                     /locus_tag="ACG0BW_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFK7261787.1"
                     /translation="MTDGISTSPHCLYKSNIADDVIINKTRQNELVKVFCEYKTEFLI
                     LFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHFYRCKLIEHTVQFSFFKHKEITLLRLD
                     VFDDRTSECLSEEIKIYQECYEKFVKFLKANFNQEIYPQLYTPEIFYEACRNLQSFYD
                     HQETSQNANYSAIDKKKSYFNKEIK"
BASE COUNT          193 a           85 c          100 g          239 t
ORIGIN      
        1 tttgatttcc ttattaaaat aacttttttt cttgtctatt gcactgtaat tagcgttttg
       61 tgacgtctcc tgatgatcat aaaatgactg gaggtttctg caggcctcat aaaatatttc
      121 aggagtatat agctgtgggt atatttcttg gttgaagttt gcctttaaaa acttaacgaa
      181 tttttcatag cattcttgat atatcttaat ttcttcagaa agacattcgc ttgttctgtc
      241 atcaaaaaca tccaatctaa gtaatgtgat ttctttgtgt ttaaaaaaag aaaattgaac
      301 tgtatgttct atgagtttac atctataaaa atgcgcatct cttagatgag tgtattgaaa
      361 aaaatgatga aggactggag atggtttagg caaatcctgg cttctaaaaa agtcatcaaa
      421 caaaattaag aactctgttt tatactcgca aaacactttt actaactcat tttgtcttgt
      481 tttatttatt attacatcat ctgctatatt tgatttataa agacaatgtg gcgaagttga
      541 gataccatct gtcattatca gtccttgaag ttgatgaatt tttattaatt taatttatga
      601 gtgaatgtga ggactat
//
LOCUS       DAYPHN010000399          614 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000399 DAYPHN010000000
VERSION     DAYPHN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.408"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..614
                     /locus_tag="ACG0BW_004970"
                     /pseudo
     CDS             1..614
                     /locus_tag="ACG0BW_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          174 a          150 c          158 g          132 t
ORIGIN      
        1 caccagcctc tcacactgga ttaatctttt tcttcttcac ggtcctcggg cactggattg
       61 cagacacaag cgtagctatt ctcctgaaga taaactttgc gtggttcttt atgctctcgg
      121 gcattctgag tctctacccc gggtagctgc ccggtttaac attcccagcc acaacacggt
      181 taaaaactgg ataaaaggct accgcaaatc tggtaatgag gcctttatca ggcgcaggaa
      241 ggagaaaagc atgacccgtt ctgatgatac ccatgaaaac gaggcaaaca tgacgccgga
      301 agagatgaag aatgagctgc gttatcttcg cgcagaaaat gcatatctga aagccatgca
      361 ggagcatctc ctggaaaaaa agcgccagga gctggagaaa aaacgaaagt catccggagc
      421 ctgaggtgcg gacactgcca gtctgacctg ttaaaggctg cagggctggc cagaagcacg
      481 ctgtactacc agctgtcact gcagaaggct aaggacaagt atgctgatgt gaaacagctc
      541 atagcctcca tcttccatga acaccgggga tgttatgggt accggcggat ccattgtgag
      601 cttcagaaaa gagg
//
LOCUS       DAYPHN010000400          601 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000400 DAYPHN010000000
VERSION     DAYPHN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.409"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>601)
                     /locus_tag="ACG0BW_004971"
     CDS             complement(<1..>601)
                     /locus_tag="ACG0BW_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HFK7261788.1"
                     /translation="SVNNAPLSLNINNSVDNWIAYNKRLLDFFLENKERCVLINFEAF
                     QGNKKNIIKPLSNVIKIDNLMSAHYKNSILFDVVENNDYTKSNEIALLEKYTDLFSLS
                     ANETEITFNDTKVSEYLVSELIKERTEVLKLYNELQAYANLPYIETSKDNVSAEAALW
                     EVVEERNSIFNIVSHLVQESKKKDADIELTKSIFKKRQFL"
BASE COUNT          195 a          109 c           68 g          229 t
ORIGIN      
        1 ataaaaattg tcttttctta aatatagatt tagtcaactc aatatctgca tccttctttt
       61 ttgactcttg caccaaatga gatacaatat tgaagataga atttctctct tcgactacct
      121 cccataatgc agcctcagcc gaaacgttat ctttcgatgt ttctatataa ggtaggtttg
      181 cataggcctg taactcatta taaagcttca gaacctcggt tctttctttt attaattcag
      241 atactaagta ctcactaacc tttgtatcat taaatgtaat ttcagtctca tttgcactta
      301 aagaaaataa atctgtatat ttttcaagca gggcaatttc atttgatttt gtataatcat
      361 tattctcaac cacatcaaac aatattgaat ttttgtaatg cgcagacatt agattatcta
      421 tttttataac attactcaat ggctttataa tatttttctt attgccttga aacgcctcaa
      481 aattaatcaa cacacatcgt tctttattct ccaaaaaaaa atcaagcaat cttttattat
      541 atgcaatcca gttatctaca ctattattta tatttaaaga gagaggggca ttatttactg
      601 a
//
LOCUS       DAYPHN010000401          597 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000401 DAYPHN010000000
VERSION     DAYPHN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.410"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(4..>597)
                     /locus_tag="ACG0BW_004972"
     CDS             complement(4..>597)
                     /locus_tag="ACG0BW_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFK7261789.1"
                     /translation="WGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKR
                     EYKEQEGSPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIP
                     LISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLL
                     WLEDVENAGQPVPDEQFSSEDKFIEGEDTEIENKDNN"
BASE COUNT          170 a          119 c           94 g          214 t
ORIGIN      
        1 tattcaatta ttatctttat tctcgatttc tgtgtcttca ccctcaatga atttatcttc
       61 tgaagagaac tgttcatctg gaacaggttg tccggcattc tccacatctt caagccacag
      121 cagaagacgt aatatttcat ctatattttc gaagctcaca taatcgtagc gacgatgggt
      181 agcataaatt tttcgtgcta attttttatc tgtaataatt ggtatcccga tttcctttgc
      241 atatttcctt acggctaatg ctacctcatt agtttctctt acagaaatca atgggatagg
      301 tgacagatgt ggcttaaagt atatccctat ggcaatgtga gtagggttgg caatcatcaa
      361 acggctatta ctgacatcag atttcaattg ctcagaaaga atttcttgat ggcgctctct
      421 gcgtttagac ttaatttctg gacttccttc ttgttccttg tattctcttt taacttcttg
      481 tttatccatt ttcatatctt tcataaataa aaaatattca gcgataaaat caaaaattaa
      541 gacaattatc atactgccga gacaatacag tatgagaagg aatagcatct ctcccca
//
LOCUS       DAYPHN010000402          595 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000402 DAYPHN010000000
VERSION     DAYPHN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.411"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            241..534
                     /locus_tag="ACG0BW_004973"
                     /pseudo
     CDS             241..534
                     /locus_tag="ACG0BW_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(517..595)
                     /locus_tag="ACG0BW_004974"
                     /pseudo
     CDS             complement(517..595)
                     /locus_tag="ACG0BW_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          188 a          106 c          130 g          171 t
ORIGIN      
        1 cgtagttaaa tgctcaaaga aaaagaatcg atattaataa aaataagttt gttttctgaa
       61 gtagtgaaaa tagcggatgg tgaaagatat taatatcaaa aatcgctcat cacggatata
      121 atgatgcacc cagggaggga gatgactgac aacgccttac accatacatt cttgttgtgc
      181 ggtatgtaat tattctacgc tgtcggtatc ggtgcgtttg ttaatcagca ggttaaccag
      241 ccacaaaaag ccattgaaaa aatcatcgat tttacctggg attattatat tgctaatccc
      301 tggtttttaa aaattgtgca tagtgaaaac caaagtaaag gtgtgcatta tgcaaaatca
      361 cagcgtctct tagaaattaa tcatgcgcac ttgcagttaa tggaatcctt attggatgaa
      421 gggaggaaac acaatatttt taagccagat atcgacccat tgcaggtgaa tattaatatt
      481 gctgcgcttg ggggatatta tttgatcaac cagcatacgc ttggcctggt ttatccggtg
      541 cgccgtaaaa ccccgtcctt cagggcgggg atataaggcg cggttttcca cctga
//
LOCUS       DAYPHN010000403          592 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000403 DAYPHN010000000
VERSION     DAYPHN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.412"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..116
                     /locus_tag="ACG0BW_004975"
     CDS             <1..116
                     /locus_tag="ACG0BW_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFK7261790.1"
                     /translation="NLRQHLARLGRKSLSFSKSVEQHDKVIGHYLNIKHYQ"
     gene            132..461
                     /locus_tag="ACG0BW_004976"
                     /pseudo
     CDS             132..461
                     /locus_tag="ACG0BW_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
BASE COUNT          133 a          153 c          174 g          132 t
ORIGIN      
        1 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
       61 tggagcagca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
      121 gagtcattac cgccaacctg ttcccggcaa ccttcagcga cggatacgtt gatatctacg
      181 gctatcatct gccgcgcccg ctgcactttg gtacacaggg tgaagggatg gtcggtgtgc
      241 gcccggaagc gatcacgctc agcgatcgcg gcgaagagag ccagcgctgc gtgatccgcc
      301 atgtcgccta tatggggccg cagtatgaag tgacggtgga atggcacggg caggagatat
      361 tattgcaggt caacgctacg cgtctgcaac cggacgtcgg cgagcagtat tatcttgaaa
      421 tccatccgta cggcatgttt gttctggcgg atgcggcata acgttttgtg gttttgtagg
      481 ccggataagg cgcttgtgcc gccatccggc aatgcctgat gcgctgcgct tatcaggcct
      541 acaatgaacg atgtagaccc gataaaccct cagcttcgcg cttttaacgc gg
//
LOCUS       DAYPHN010000404          590 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000404 DAYPHN010000000
VERSION     DAYPHN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.413"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..558
                     /locus_tag="ACG0BW_004977"
     CDS             <1..558
                     /locus_tag="ACG0BW_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFK7261791.1"
                     /translation="KIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAII
                     EVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYS
                     FTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT          159 a          146 c          154 g          131 t
ORIGIN      
        1 aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa
       61 gtacaggctt cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact
      121 caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat
      181 gatgaagatt accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc
      241 gttgccagag ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt
      301 caggaagatc aactgggcgc actggggctg gtcactaacg ccgtcgtgtt atggaacact
      361 atttatatgc aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa
      421 gatatcgcac gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc
      481 ttcacgctgg cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag
      541 gcagaaaacg ttgcttaacg tgagttttcg ttccactgag cgtcagaccc
//
LOCUS       DAYPHN010000405          589 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000405 DAYPHN010000000
VERSION     DAYPHN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.414"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>589
                     /locus_tag="ACG0BW_004978"
     CDS             <1..>589
                     /locus_tag="ACG0BW_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFK7261792.1"
                     /translation="FYMCDFSSSDMTCKRNGIRKDLLDFDPREYFKKDGGKLNLDKHI
                     SNLIYVNLFPNMSGGRYINDKYVADDIDKYLKLSNCNLLKTCLGESINGIKLDDCAID
                     PSTTWLSGNLAANSQIRIPVYMDMKGAIAENGISDFMENLMEVNSWVEEFNNYGKDAI
                     SHYEKELLYLQSLLVKLGSFNLDDEGKKYQLNGEAI"
BASE COUNT          201 a           79 c          115 g          194 t
ORIGIN      
        1 ttttatatgt gtgattttag ctcctctgat atgacatgca aacgaaatgg aattcgcaaa
       61 gatttactgg attttgatcc cagagaatat ttcaaaaagg atggaggtaa acttaatctg
      121 gataaacaca tttcaaacct tatctacgtt aatcttttcc ctaatatgag tggcggtcgt
      181 tatataaatg ataaatacgt tgctgatgat atagataaat atttaaaact tagtaattgc
      241 aatttactta aaacctgttt gggcgagtca atcaatggaa ttaaattgga tgattgtgct
      301 attgatcctt caaccacttg gttaagtggt aatcttgctg cgaatagcca gattagaatt
      361 cctgtttata tggacatgaa aggagctata gctgaaaatg gcatttcaga tttcatggaa
      421 aatttaatgg aagttaattc atgggtagaa gaattcaata attatggcaa ggatgcgata
      481 agtcattacg aaaaggaact attatattta caatctttgt tggtcaagct gggtagcttc
      541 aatcttgatg atgaaggcaa aaaatatcaa cttaatggtg aagctatcc
//
LOCUS       DAYPHN010000406          587 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000406 DAYPHN010000000
VERSION     DAYPHN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.415"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..311
                     /locus_tag="ACG0BW_004979"
     CDS             <1..311
                     /locus_tag="ACG0BW_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFK7261793.1"
                     /translation="GGLIVRHESYSDPNEYDKSDFDPIKAAGWIDSKWITEVYEPLVN
                     KRLCPVGVGFSEHMTYFISDDGALYGGFDNYFCIIGGNINDAMLHLFFEHEKIKTLSS
                     "
     gene            398..>587
                     /locus_tag="ACG0BW_004980"
     CDS             398..>587
                     /locus_tag="ACG0BW_004980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261794.1"
                     /translation="MTDSLGHTTIIRFSEDRLPVCEIDPEGGATFFLYDEFGQTVSIT
                     SQGKIIRSTLFVLNNSHFM"
BASE COUNT          155 a          100 c          137 g          195 t
ORIGIN      
        1 ttggagggtt gatagtacgg catgaatctt attcagatcc taatgagtat gataaatccg
       61 attttgatcc aataaaagct gctggttgga tagactctaa atggataact gaagtctatg
      121 aaccattggt caataagcgt ttatgtcctg ttggtgttgg tttttctgag catatgacat
      181 attttatttc agatgatggt gctctttatg gtggttttga taactatttt tgtattatag
      241 gcggtaatat taatgacgct atgctacatt tatttttcga acacgaaaaa ataaaaacct
      301 tgtcaagtta atttgattcg atgattgatt gctgttgggc gacggcgggt actacgacta
      361 ccactttgta tggaatgatt tctgccatga gctggagatg actgattcgc tggggcatac
      421 caccattatc cgcttcagtg aagaccggct tccggtgtgt gaaattgacc cggagggcgg
      481 cgcgacgttt ttcctctatg acgaattcgg gcagacggtc agtatcacct cccagggaaa
      541 gatcatcaga agtacacttt ttgtactaaa taattcgcat tttatgt
//
LOCUS       DAYPHN010000407          567 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000407 DAYPHN010000000
VERSION     DAYPHN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.416"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(261..335)
                     /locus_tag="ACG0BW_004981"
                     /pseudo
     CDS             complement(261..335)
                     /locus_tag="ACG0BW_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          185 a          102 c          120 g          160 t
ORIGIN      
        1 cacttaatga tgtagttcaa aatatcagga gttggctaaa agaaaatgaa aatatggtga
       61 aagctattaa gaaaattgat actgaataac atggcgaata aataaacgga tcaagtcgtt
      121 cctgtgacaa tgaggttata cgaaaagacg cttactcatc agaaatttgg tatgctttaa
      181 ttaaaccagt gtcgctgatg tgtaagcgtt gacgttggga ggcgattaaa tggttatacc
      241 actcctacgc cttttccaga cggcgtataa gagtgcaaca tgttgagttt tccgagcgaa
      301 tggtgcattt tactaaccga gccatttgaa acaaccaacg caataaaaaa gactggaata
      361 ataagcctgt atttattatt catcgcaatc gcactctacg gttttatcct gcagaaatga
      421 gactacttcc agcggttgtg gattatccct gggcgatagt catagtctgg tgtcaggatt
      481 cacaaatgac ctgttacctg acatggatat aaacacaaca tagagtgcgg tagttaatct
      541 gaatcatcta tttttcggct acacagc
//
LOCUS       DAYPHN010000408          554 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000408 DAYPHN010000000
VERSION     DAYPHN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.417"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..344
                     /locus_tag="ACG0BW_004982"
     CDS             <1..344
                     /locus_tag="ACG0BW_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFK7261795.1"
                     /translation="VHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESNSDS
                     APYTLLKNYADRFKKYSAEEQYEIDFQHRQANRNCYLDKYGDANINDYYRDLEIKHSQ
                     PQNRASGKRVS"
BASE COUNT          184 a          109 c          105 g          156 t
ORIGIN      
        1 aagttcattt aactgaaccc gagataatat ttttacatgc cgatcttcag caatatttaa
       61 gccaaagttg cggtgcattt gtgtgcatgg cagcccagga agtaattgaa caaagggaaa
      121 gcaattctga cagcgccccc tatacgttat taaaaaacta tgctgacaga tttaaaaaat
      181 attcagcaga agagcagtac gaaattgatt ttcaacatcg acaggcaaac agaaattgtt
      241 atttagataa atatggcgat gcaaatatca atgattatta tagagactta gaaataaaac
      301 actcacaacc ccaaaataga gcatcaggca aaagagtaag ttaacatcat tgttaattaa
      361 ttcgaccagt cagcaaatat gctgactggt cgaacatagt ttgctaaaaa gtcatcactg
      421 actgatgtca gtcacaccac ccgccagttc aatctgcggg tatttccagc gtatcgcggc
      481 actcgattgc tggtctgttg atgtagtgaa ttgttgttgc tgatttttct taccctcaga
      541 tttctgcacc ctgt
//
LOCUS       DAYPHN010000409          545 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000409 DAYPHN010000000
VERSION     DAYPHN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.418"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            340..>545
                     /locus_tag="ACG0BW_004983"
     CDS             340..>545
                     /locus_tag="ACG0BW_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFK7261796.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHR"
BASE COUNT          142 a          151 c          127 g          125 t
ORIGIN      
        1 cagtgccagc agtatggtaa cgatacggtc tgtattccgg taatagccga aggggtcgac
       61 agaaccacaa tacgggcaac acacagtatt atgagtaatc gcgtttccac agtcccagca
      121 cttctttttg cgtatcacca taactccctc ctcacagcct tacccgtaac cggtttttta
      181 cattaaaaaa acaacgctga aaattcaacc gtcagtccgg agacgatagt tggggtatca
      241 cagagtgcct gagacagtgt cctgccggag gtcacttcag ggactctgcg cattttttac
      301 gacgtggcta ttccgtatga accatacgga gatttaacca tgacctacaa atacaacccc
      361 ttctggcagc aacgtattcg tgagacggtg cggcacgcac tgaatgttca tccccgcctg
      421 acggcattgc gggttgacct gcgtttcccg gatgtaccgg cagcaacgga cgcagctgtg
      481 atatcccgct tcatcaatgc cctgaaagcc cgaatcgacg cttaccagaa acgtaagcat
      541 cggga
//
LOCUS       DAYPHN010000410          543 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000410 DAYPHN010000000
VERSION     DAYPHN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.419"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>543
                     /locus_tag="ACG0BW_004984"
     rRNA            <1..>543
                     /locus_tag="ACG0BW_004984"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          124 a          121 c          183 g          115 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccg
//
LOCUS       DAYPHN010000411          542 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000411 DAYPHN010000000
VERSION     DAYPHN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.420"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            51..>542
                     /locus_tag="ACG0BW_004985"
     CDS             51..>542
                     /locus_tag="ACG0BW_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFK7261797.1"
                     /translation="MEKMFQILETAWPAVKTTGLTGCVTAPETEDNLLLFEDKYATHI
                     PKDYRQLLSHFGACHFVDPQIYTLKDLDRYYPLFLQQWEDYKKEYPLLNNIDPFPVGS
                     FGDGSVAFLDRNSLKIYILIHDFGIFSDDVGDFPCELCFENFGELVEMQVEIILYLQQ
                     CGVS"
BASE COUNT          164 a           93 c           98 g          187 t
ORIGIN      
        1 aaattgtcct ttttattcat caacataata ctatatgtcg agaaacatac atggaaaaaa
       61 tgtttcaaat tttagaaacc gcctggcctg ccgtaaaaac aacaggactt acaggttgcg
      121 taaccgctcc ggaaactgaa gataaccttc ttttatttga ggataaatac gcaacacata
      181 ttccaaaaga ctatcggcag ttattgtctc attttggcgc atgtcatttt gtcgatcctc
      241 aaatttatac tttgaaggat ctggacagat attaccctct atttttgcaa caatgggaag
      301 attataaaaa agaatatcca ttattgaaca acattgatcc ttttcctgtt ggcagttttg
      361 gcgatgggag tgtagctttt cttgaccgca acagcctaaa aatttatatt ctcattcatg
      421 attttggtat attttccgat gatgtcggtg atttcccttg tgagttatgc tttgaaaatt
      481 ttggtgagtt agtggaaatg caagttgaaa ttattcttta tttacagcaa tgtggagtct
      541 ca
//
LOCUS       DAYPHN010000412          542 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000412 DAYPHN010000000
VERSION     DAYPHN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.421"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            228..461
                     /gene="papI"
                     /locus_tag="ACG0BW_004986"
     CDS             228..461
                     /gene="papI"
                     /locus_tag="ACG0BW_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HFK7261798.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQSCSSTT"
BASE COUNT          199 a           94 c          113 g          136 t
ORIGIN      
        1 atcatggtga attgaattta cagcataaaa gatcgtctaa atgcattttt acggactttc
       61 ttttcacaga aaaataacac acaaaatgtg atacacatca aataaagcca acaaaaagca
      121 aattcactaa attgcacaaa taaagcaaac acagagaata aaaatacctt tccgttgata
      181 acagagagca tgatttttat tattcgaaga aactttgtta cagtgagatg agtgaatata
      241 tgaagaatga aatactggaa tttctgaaca ggcataatgg agggaaaacc gcagaaattg
      301 cggaggcgct ggcggtaacg gattatcagg cacgctatta cctgttatta ctggaaaaag
      361 aaggtgtggt tcagcgctct ccattaaggc gaggcatggc aacatactgg cttctgaaag
      421 gagagaagca ggcggggcag agctgttctt ccacaactta ataactcaat ctgtgggcat
      481 gcttcactga aacagataaa agttacatca gagtgcagat tcaattttgc agtattcatc
      541 ac
//
LOCUS       DAYPHN010000413          521 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000413 DAYPHN010000000
VERSION     DAYPHN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.422"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..521
                     /locus_tag="ACG0BW_004987"
                     /pseudo
     CDS             1..521
                     /locus_tag="ACG0BW_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          116 a          141 c          147 g          117 t
ORIGIN      
        1 aaccagcctc atgcgcagtt ctggctggtt tttgacgttg atcgcgaggg ggcagcgatt
       61 gactggagcg accggaacgc tcccgcgccg aacatcaccg taaaaaatcc tgtgaacggt
      121 catgctcacc tgctctacgc gctcaatatc gccgtgagaa ccgcgccgga cagctcggtt
      181 aaggcgctga aatacgccgc tgcgattgaa cgttcgctgt gtgagaaact ttgtgctgat
      241 gtgaactaca gcggactgat ttgtaaaaat ccgttccacc tggaatggca ggtgatggag
      301 tggcgagagg aagcctatac cctcgatgaa ctggctgatt atctcgattt gagcgcctca
      361 gcgcgccgta gcatcgacga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca
      421 gggcgattcg tcagggctgg cccgaattct cacagtggct tgatgccgtg attcagcgtg
      481 tcgaaatgta caacgcatcg cttcccgttc cgctttcacc g
//
LOCUS       DAYPHN010000414          516 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000414 DAYPHN010000000
VERSION     DAYPHN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.423"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
BASE COUNT          132 a           80 c          123 g          181 t
ORIGIN      
        1 ttttaattcc tgtactgaaa cactgtcgct cgtccggggt taactgaaac tggatgatga
       61 tgtaaaatga aggctgtttt tgtgtttgtc gaagaagtgt tattttaatc cattctcatc
      121 attggctggc gattatacag aagtatttga agaaaaaatg gtgcatttta tctgcgtcag
      181 cagggaggaa gtattatatt gatgtatcag tattaaatgg tttttgttat ttataaatat
      241 gtcatcctgt ccatagaata ttgtactcag cattcagtgc aggaactgta gtcttcttta
      301 ttttattctt cttcaggagg ggggggattc ctgaaggcgt tattcccggt gttctgttta
      361 tgattcatcc cggcaggaaa ccggtatctg caatacccgg gatgatgctt tttgttcgta
      421 cgtggttgtg taaaatttga atagtggatg tgctctgaag ggaaggaggg gacacacaga
      481 ttcattttgt ctttcttcag cagcaaaaca cactgt
//
LOCUS       DAYPHN010000415          514 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000415 DAYPHN010000000
VERSION     DAYPHN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.424"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(89..>514)
                     /gene="tnpA"
                     /locus_tag="ACG0BW_004988"
     CDS             complement(89..>514)
                     /gene="tnpA"
                     /locus_tag="ACG0BW_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFK7261799.1"
                     /translation="WNCKYHIVFAPKYRRQVFYREKRRAIGSILRKLCEWKSVRILEA
                     ECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRNREFWCRGYYVD
                     TVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          121 a          150 c           89 g          154 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccactgtat cgacgtagta ccctctgcac cagaactccc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaac ccggatacgc tcattttagg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcca gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacctgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tcca
//
LOCUS       DAYPHN010000416          513 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000416 DAYPHN010000000
VERSION     DAYPHN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.425"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            314..>513
                     /locus_tag="ACG0BW_004989"
     CDS             314..>513
                     /locus_tag="ACG0BW_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFK7261800.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKV"
BASE COUNT          136 a          134 c          110 g          133 t
ORIGIN      
        1 gatagtttcc taatcgccaa tgaatataag caccatcatt tctccctatt tttatgttag
       61 aagtgacaaa gatttgtagg gtgatgcaga gctgacaatc acaggtttcc ttatcggttt
      121 ttgcatcgca caactaaagc aataaacgag ctcccgtcat cggcgaatat gaattaatta
      181 gccgggctca tagctgcatt cgcgttttaa gaataatcct cctgctctca ccgactatgc
      241 ttaacgttta aaaaagcatc agcactctcg caacgctctc ttattttccc ctttagaata
      301 ccggaggcct ggtatgagca accaaggcga ataccccgaa gataatcggg ttgggaagca
      361 cgagccgcac gattttagtt tgacccgtcg cgatctgatt aaagtcagcg ccgcaacggc
      421 ggcgaccgcc gtggtttatc ctcattctac gcttgcagca agcgttccgg cagctacacc
      481 cgcgccagag ataatgcccc tgacacttaa ggt
//
LOCUS       DAYPHN010000417          507 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000417 DAYPHN010000000
VERSION     DAYPHN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.426"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>507)
                     /gene="wzy"
                     /locus_tag="ACG0BW_004990"
     CDS             complement(<1..>507)
                     /gene="wzy"
                     /locus_tag="ACG0BW_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O15 family O-antigen polymerase"
                     /protein_id="HFK7261801.1"
                     /translation="FGMYISPELKRLLYTFFGMSDSVNLWEQNAKVRGFGLSGEINFM
                     TPFLMIYMSFFMMKRRYALITLICLTQIVNSNMAVIAAIIGIGCSRLNINIKIATVLI
                     LGVLVYSLGAVFFPRFYDEFVSGDGTRTLDILLQQHVFVVGNLDFFNIIFGLQQNISS
                     SIPDIKQS"
BASE COUNT          201 a           95 c           63 g          148 t
ORIGIN      
        1 aactttgttt aatatcgggg attgatgaag atatgttttg ctgtaatcca aatataatat
       61 taaaaaaatc taaattacct acaacaaaca catgttgctg taataagata tccagagttc
      121 ttgtgccatc tccagaaacg aactcatcat aaaatcgagg aaagaacacc gctcctaagc
      181 tataaactaa aactcccaaa atcaatactg ttgcaatttt tatattaata ttaagtctag
      241 agcaaccgat accaataatg gctgcaatca cagccatgtt agaatttacg atttgagtca
      301 gacaaattaa agtaattaaa gcataacgcc ttttcatcat aaaaaatgac atatagatca
      361 tcaaaaatgg tgtcatgaaa tttatttcac ccgacaaccc aaatcctctt actttagcat
      421 tttgttccca aagattaaca gagtcagaca taccaaagaa agtatataac aatcttttta
      481 actctggact tatatacata ccaaaaa
//
LOCUS       DAYPHN010000418          496 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000418 DAYPHN010000000
VERSION     DAYPHN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.427"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>496
                     /gene="kfiA"
                     /locus_tag="ACG0BW_004991"
     CDS             <1..>496
                     /gene="kfiA"
                     /locus_tag="ACG0BW_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis alpha-UDP-GlcNAc
                     glycosyltransferase KfiA"
                     /protein_id="HFK7261802.1"
                     /translation="CAKNDMIVLTDDDIIYPPDYVEKMLNFYNSFAIFNCIVGIHGCI
                     YIDAFDGDQSKRKVFSFTQGLLRPRVVNQLGTGTVFLKADQLPSLKYMDGSQRFVDVR
                     FSRYMLENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKETQAIAGYSKL
                     NLELV"
BASE COUNT          169 a           75 c           94 g          158 t
ORIGIN      
        1 tgcgctaaaa atgatatgat cgtacttaca gatgatgata ttatttaccc tcccgattat
       61 gtagaaaaaa tgctcaattt ttataattcc tttgcaatat tcaattgcat tgttgggatt
      121 catggctgta tatacataga tgcatttgat ggagatcagt ctaaaagaaa agtattttca
      181 tttactcaag ggctattgcg accgagagtt gtaaatcaat taggtacagg gactgttttt
      241 cttaaggcag atcaattacc atctttaaaa tatatggatg gttctcaacg attcgtcgat
      301 gttagatttt ctcgctatat gttagagaat gaaattggta tgatatgtgt tcccagagaa
      361 aaaaactggc taagagaggt ctcatcaggt tcaatggaag gactttggaa cacatttaca
      421 aaaaaatggc ctttagacat cataaaagaa acacaagcaa tcgcaggata ttcaaaactt
      481 aacctcgaat tagtgt
//
LOCUS       DAYPHN010000419          485 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000419 DAYPHN010000000
VERSION     DAYPHN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.428"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>485
                     /locus_tag="ACG0BW_004992"
     rRNA            <1..>485
                     /locus_tag="ACG0BW_004992"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAYPHN010000420          484 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000420 DAYPHN010000000
VERSION     DAYPHN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.429"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            144..>484
                     /gene="panM"
                     /locus_tag="ACG0BW_004993"
     CDS             144..>484
                     /gene="panM"
                     /locus_tag="ACG0BW_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFK7261803.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRTNPGVLGWWMADAGV
                     EDRGVMTAFMQA"
BASE COUNT          118 a          111 c          124 g          131 t
ORIGIN      
        1 atttttcatt ctctaatgtt ttaactttgt aattattgct gaaaaaaaat taatcacctg
       61 ccaaaagaaa taaaaaagag aaagcctccg attaaattat ttcgctacac tggttcctct
      121 tttgtgattt acacgggtta cccatgaagc tgaccatcat tcgattagaa aactttagcg
      181 accaggaccg gattgacctg caaaagatct ggccggagta ttccccttcc tctttacagg
      241 ttgacgataa ccaccgtatc tacgccgcgc gttttaacga gcgcctccta gcggccgtgc
      301 gggtaacctt aagcggcacc gagggggcac tggattctct acgtgtgcgg gaagtcaccc
      361 gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt gcgtaccaat cctggcgttt
      421 taggctggtg gatggcggat gctggcgtgg aagatcgcgg tgtgatgaca gcgtttatgc
      481 aggc
//
LOCUS       DAYPHN010000421          482 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000421 DAYPHN010000000
VERSION     DAYPHN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.430"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            39..107
                     /gene="ykiE"
                     /locus_tag="ACG0BW_004994"
     CDS             39..107
                     /gene="ykiE"
                     /locus_tag="ACG0BW_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFK7261804.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            194..459
                     /locus_tag="ACG0BW_004995"
                     /pseudo
     CDS             194..459
                     /locus_tag="ACG0BW_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
BASE COUNT          112 a          116 c          118 g          136 t
ORIGIN      
        1 acatgttgtt tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct
       61 ggcatgtgtc ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc
      121 ctaaaggatg gggatttcgg taatgctgcc aacttactga tttagtgtat gatggtgatt
      181 ttaaggtgct tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg
      241 cgtggtgcgt aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg
      301 taaaacatgg caactacagt tcacttacac cgcctctcag cccggtacgc accagaaaat
      361 cattgatatg gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg
      421 cctcaacacg gttttacgtc acttaaaaaa ctcaggccgt agtcggtaac ctcgcgcata
      481 ca
//
LOCUS       DAYPHN010000422          481 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000422 DAYPHN010000000
VERSION     DAYPHN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.431"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            111..>481
                     /locus_tag="ACG0BW_004996"
     CDS             111..>481
                     /locus_tag="ACG0BW_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFK7261805.1"
                     /translation="MRDLAQKCDIPYSTIRGYVAGDSLPNGIEQLQKIAHATGVSPAW
                     LLGEETGQSQDSERVKRELGLLMTLAEHLSDEQRGIFIKKMLNALLYQVEGVERTSDE
                     ERKMLSASSIELALKINKLPI"
BASE COUNT          152 a           82 c          108 g          139 t
ORIGIN      
        1 tagttcatct gtttttctta tcattcatat ggttacaaaa tcggagtatg atgattaaga
       61 gtgaatttag tgccagatta gagaaacttc tcgctatgac aggcgattcc atgcgtgatc
      121 tggcacaaaa atgtgatatc ccgtactcta cgatccgtgg atatgttgcc ggagattcgc
      181 ttccgaatgg aattgagcaa cttcagaaga tcgctcatgc tactggagtt agtccggctt
      241 ggttactagg tgaggaaaca ggtcaatctc aagatagcga acgagtaaaa agggaattag
      301 gtctactaat gactttggct gaacatttgt ctgatgaaca gcgaggtata tttataaaaa
      361 agatgctaaa tgctttgctc taccaagttg aaggtgttga acgaacttca gatgaagaaa
      421 gaaagatgtt atcggcatcc tctatcgagt tggccttaaa aatcaataaa ttaccaatca
      481 a
//
LOCUS       DAYPHN010000423          480 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000423 DAYPHN010000000
VERSION     DAYPHN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.432"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..80)
                     /locus_tag="ACG0BW_004997"
     CDS             complement(<1..80)
                     /locus_tag="ACG0BW_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFK7261806.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSD"
     gene            complement(91..303)
                     /gene="cspI"
                     /locus_tag="ACG0BW_004998"
     CDS             complement(91..303)
                     /gene="cspI"
                     /locus_tag="ACG0BW_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFK7261807.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVTL"
BASE COUNT          171 a           98 c           79 g          132 t
ORIGIN      
        1 ctgtcagaaa catcaaaaga tgatgttcta tactgagaac catggcaaca cgggcatcta
       61 aagtgaatat ggtttgtaat attttctacc tcaaagtgtc actacatgaa cagctgcagg
      121 gcctttaggt ccgttctcaa taccaaattc gacttcctga ttctcagtta acgttttgaa
      181 atcgttgctc tgaatagctg agaaatgcac aaacacatct ttgctaccat ctttcggcgt
      241 gatgaaacca aaaccttttt cagggttaaa ccatttcact aaaccagtca ttttgttaga
      301 catcattatt accttttgaa gaagttagcc cttgggcaga atggtccgaa aaaaaatatc
      361 agagagaaaa accaacaagg aaatctcaag aggtacaaat aataaaatta taacaatgac
      421 tgcttcagat aattttgtaa caaaccagaa caccattaac gcatgattaa ccaaccatag
//
LOCUS       DAYPHN010000424          475 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000424 DAYPHN010000000
VERSION     DAYPHN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.433"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..184
                     /locus_tag="ACG0BW_004999"
     CDS             <1..184
                     /locus_tag="ACG0BW_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFK7261808.1"
                     /translation="GTNDFENLVLIENEPYHKVFTNMQSRIAKGVLVGESKITPWAIP
                     SGSIYPPMKNIMEPTK"
     gene            181..>475
                     /gene="yrhA"
                     /locus_tag="ACG0BW_005000"
     CDS             181..>475
                     /gene="yrhA"
                     /locus_tag="ACG0BW_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFK7261809.1"
                     /translation="MMTKTQINKLKKMMNDLDYPFEAPLKESFIESIIQIGFNSNSTN
                     CLEKLCNEVSILFNNQPDYLNFLRAMDGFEVNGLRLFSLSIPEPSVKNLFTVNE"
BASE COUNT          180 a           76 c           69 g          150 t
ORIGIN      
        1 tggtactaat gatttcgaaa atttagtatt aatcgaaaac gaaccatatc ataaagtttt
       61 tactaacatg caatcacgaa tagctaaggg agtattagta ggtgaaagca aaatcactcc
      121 ctgggccatt ccatctggct caatttatcc tcccatgaaa aatattatgg aacccacaaa
      181 atgatgacta aaacccaaat aaataaatta aaaaaaatga tgaatgattt agactatcca
      241 tttgaagcac cgctcaagga atcatttatt gaaagtataa tccaaatagg atttaattct
      301 aattcaacta attgcctgga gaaattatgt aatgaagtta gtattctttt taataatcaa
      361 cctgattatc ttaatttttt aagagcaatg gatggattcg aagttaatgg attacgatta
      421 tttagcctct cgattccaga accttcagtt aaaaaccttt ttaccgtaaa tgaat
//
LOCUS       DAYPHN010000425          472 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000425 DAYPHN010000000
VERSION     DAYPHN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.434"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>472)
                     /locus_tag="ACG0BW_005001"
     rRNA            complement(<1..>472)
                     /locus_tag="ACG0BW_005001"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           90 a          151 c          100 g          131 t
ORIGIN      
        1 agttacggca ccattttgcc tagttccttc acccgagttc tctcaagcgc cttggtattc
       61 tctacctgac cacctgtgtc ggtttggggt acgatttgat gttacctgat gcttagaggc
      121 ttttcctgga agcagggcat ttgttgcttc agcaccgtag tgcctcgtca tcacgcctca
      181 gccttgattt tccggatttg cctggaaaac cagcctacac gcttaaaccg ggacaaccgt
      241 cgcccggcca acatagcctt ctccgtcccc ccttcgcagt aacaccaagt acaggaatat
      301 taacctgttt cccatcgact acgcctttcg gcctcgcctt aggggtcgac tcaccctgcc
      361 ccgattaacg ttggacagga acccttggtc ttccggcgag cgggcttttc acccgcttta
      421 tcgttactta tgtcagcatt cgcacttctg atacctccag catacctcac ag
//
LOCUS       DAYPHN010000426          471 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000426 DAYPHN010000000
VERSION     DAYPHN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.435"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>471
                     /locus_tag="ACG0BW_005002"
     CDS             <1..>471
                     /locus_tag="ACG0BW_005002"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFK7261810.1"
                     /translation="LSAKRNFGASLAHGDYLIFLDDDCIPASGYISSLLNYLKKMNSK
                     SVLCGEVRFENELIETSNYYRYRNSLHPKFSDSPDISMNAWTFVAMNCVLDRKAFSSG
                     IVSYNENFIGYGCEDHEFGWQLEKNDFKIIFADFKILHHEYSGDIEGYTKKIRA"
BASE COUNT          154 a           69 c           91 g          157 t
ORIGIN      
        1 tactctccgc taagcgcaat ttcggagcat ccctagccca tggggattat ttaatatttc
       61 ttgatgatga ttgtataccc gcaagtggat atatatcatc gttgctgaac tatttaaaaa
      121 aaatgaatag taaaagcgtt ttatgtgggg aagttagatt cgaaaatgaa ctcattgaga
      181 ccagcaatta ctatcgctac aggaactctt tacaccctaa gtttagtgat agtcctgata
      241 tctctatgaa tgcctggact tttgtcgcaa tgaattgtgt tcttgataga aaggcatttt
      301 catcaggtat agtttcatat aatgaaaatt ttattggtta tggttgtgaa gatcatgagt
      361 ttgggtggca acttgaaaaa aatgacttca aaattatttt tgctgatttt aaaatattac
      421 atcacgaata cagtggcgat atagaaggat atacaaaaaa aattcgtgct a
//
LOCUS       DAYPHN010000427          467 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000427 DAYPHN010000000
VERSION     DAYPHN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.436"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..260
                     /locus_tag="ACG0BW_005003"
     CDS             <1..260
                     /locus_tag="ACG0BW_005003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261811.1"
                     /translation="PVKRPQSLSGAKNAKRQENTRLNQKNAPESFLDGCITMHESDPR
                     SVLCVLNTERVKMTRFLSLNGAFYTEIFVPLSIILITGFPS"
     gene            complement(225..>467)
                     /locus_tag="ACG0BW_005004"
     CDS             complement(225..>467)
                     /locus_tag="ACG0BW_005004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261812.1"
                     /translation="KNKLIAGAVFLLNFQFVTLIITAIVIQTIIKIELPLIKSYFKIN
                     SPVFVTQSVVYVTIFAKTGRRKVLFSAWKPGNQNNG"
BASE COUNT          153 a          101 c           84 g          129 t
ORIGIN      
        1 accctgtaaa acgccctcaa tcgctttctg gcgcaaaaaa cgcaaaaaga caagaaaaca
       61 ccaggctaaa ccagaaaaac gcgccagaat cgtttttaga tggctgcata actatgcacg
      121 aaagcgaccc gcgatcggtt ttgtgtgttc tgaataccga acgggtcaaa atgacacgtt
      181 ttctctccct caatggagcg ttctatacag agatttttgt tcccctatcc attattctga
      241 ttaccgggtt tccaagctga aaacaggact tttctgcgac cagtttttgc gaatatagtc
      301 acataaacca ccgattgagt gacaaataca ggagaattta ttttgaaata tgattttatc
      361 aatggcagtt ctattttaat tatagtctgg atgactatag ctgtaataat cagagtcaca
      421 aactggaaat tcaacaaaaa aacagcccca gctataagct tattctt
//
LOCUS       DAYPHN010000428          467 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000428 DAYPHN010000000
VERSION     DAYPHN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.437"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(135..210)
                     /locus_tag="ACG0BW_005005"
     tRNA            complement(135..210)
                     /locus_tag="ACG0BW_005005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Gly,seq:gcc)
     gene            complement(246..321)
                     /locus_tag="ACG0BW_005006"
     tRNA            complement(246..321)
                     /locus_tag="ACG0BW_005006"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Gly,seq:gcc)
BASE COUNT          107 a          119 c          112 g          129 t
ORIGIN      
        1 cgtgcgaagt ttctttgaaa ttaagattac ggcctgaggc tgtggataac tctgtttaca
       61 agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt ggatatttta
      121 ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag
      181 gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgctttac agcactttca
      241 aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
      301 taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc cttggctgcc
      361 ttcactcacc ttagtcactt acttttgtaa gctcccaagg attcgttcag ttgccgcctt
      421 gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcac
//
LOCUS       DAYPHN010000429          463 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000429 DAYPHN010000000
VERSION     DAYPHN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.438"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>463
                     /locus_tag="ACG0BW_005007"
     CDS             <1..>463
                     /locus_tag="ACG0BW_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFK7261813.1"
                     /translation="SEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRGA
                     LGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNFKLA
                     LSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNILK"
BASE COUNT          134 a           81 c          103 g          145 t
ORIGIN      
        1 ttcagaggta attcagcgtc tgatccagga gcgaatttca gtgcgcaata tgcgtttagt
       61 tatggaggct ttagcattat ggagcccacg agagaaagat ataattacac tcgtcgagca
      121 tgtacgaggt gcattaggtc gctacatttg ccataagttt tcatattctg gagaaattaa
      181 agcaattgtg atttcgcctg aaatagagga cagaatcaga gatggtgtca ggcctaccgc
      241 tggcggtact tttcttaatc tcgatgcatc cgaagctgag atgattctgg ataattttaa
      301 actggctctt tctggaatca atattcctat taaagacatt attttactgg gatctgtgga
      361 tattcgtcgt tttatcaaaa aacttattga atctagttat cgagacctcg aagtactttc
      421 gtacggggaa ctaacggaaa atgttcccgt taatattctg aaa
//
LOCUS       DAYPHN010000430          457 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000430 DAYPHN010000000
VERSION     DAYPHN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.439"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>457)
                     /locus_tag="ACG0BW_005008"
     CDS             complement(<1..>457)
                     /locus_tag="ACG0BW_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7261814.1"
                     /translation="YDYSRRYAISENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFT
                     EEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYN
                     DICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDATNLVNLKTL"
BASE COUNT          144 a           96 c           70 g          147 t
ORIGIN      
        1 gtagtgtttt aagattgact aaattagttg catcttcacg taccatcgtg gcaactggaa
       61 caggcaacga catgaaaact tcttcattca ccatcaagtg gatcatgcat tgcgttccct
      121 ttgtcatatc cacacttacg ctacaaatat cgttgtaatg cgaagagtgc atttcaggaa
      181 acaaattaaa cacggtatct tgaaaggcac taaatcgtgt taataaatcc tgataatgaa
      241 caggttttgt ttcaaaatta tcatcagcaa atccaagcac tattgcatgc tctacatcta
      301 acgcagtttc aaatgactct tcttctgtaa aatcattatt taaaacctca cctgacgtat
      361 tatcactgtc ataaaaagtg ctcatcaatg cgatattgtt tgttacagcg tcttttgcga
      421 tattctcact aattgcatat cgcctgctat aatcata
//
LOCUS       DAYPHN010000431          430 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000431 DAYPHN010000000
VERSION     DAYPHN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.440"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..93)
                     /locus_tag="ACG0BW_005009"
     CDS             complement(<1..93)
                     /locus_tag="ACG0BW_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261815.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTY"
     gene            111..302
                     /gene="yfgG"
                     /locus_tag="ACG0BW_005010"
     CDS             111..302
                     /gene="yfgG"
                     /locus_tag="ACG0BW_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFK7261816.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          106 a          110 c           82 g          132 t
ORIGIN      
        1 atatgtatcc actgacgctt ttttacataa cgaagaattg accattttgt cctgttgtgc
       61 cttaatgtaa gtaccgtcca cagcgtggga catacttcaa ggaacctttt gtgagtcagg
      121 caaccagtat gcgaaaacga caccgattta acagtcgcat gacccgtatc gtactgctca
      181 tcagctttat cttcttcttt ggccgtttta tctactcgtc cgtcggtgca tggcagcacc
      241 atcagagcaa aaaagaagct cagcaatcca cactctccgt cgaatcaccg gtacaacgtt
      301 agcggttacc ttctccactt tcacagaaca taacggcacc tcgctgtcgg atgcttttgc
      361 tctttgggat tatcaaagcg gcagatattc tttcatctta aattttacgt ctttgtcctg
      421 actgatgttt
//
LOCUS       DAYPHN010000432          427 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000432 DAYPHN010000000
VERSION     DAYPHN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.441"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(190..399)
                     /gene="fumD"
                     /locus_tag="ACG0BW_005011"
     CDS             complement(190..399)
                     /gene="fumD"
                     /locus_tag="ACG0BW_005011"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFK7261817.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          124 a           97 c           67 g          139 t
ORIGIN      
        1 gaaataaatt taacttaagc gacaattatt tgtgacgttc attgcagaaa ataaaaataa
       61 cattatcgtt ataaattaaa atacataccg aaattcgtat aatttcagca gttgttattt
      121 agaatgatta acttgaccgc aactgaagca agaatatcag gatgaagctt accgcctcat
      181 cctgaatggt cattttacca gtgcgttaat gacggtttcc atcgcctgct tttccagctc
      241 actgcgctga atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg
      301 ctttggttcc tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctctgcacat
      361 ttctcggtac agttcatcct cttttgtccg atttcccatt ctttatacct atttatcatg
      421 ccaacta
//
LOCUS       DAYPHN010000433          420 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000433 DAYPHN010000000
VERSION     DAYPHN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.442"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>420
                     /locus_tag="ACG0BW_005012"
     CDS             <1..>420
                     /locus_tag="ACG0BW_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFK7261818.1"
                     /translation="HQIIENQLDTETLTVSYEFILCQHLIANTEFSYLALPENYNCLF
                     LPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNE
                     KTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT          160 a           73 c           74 g          113 t
ORIGIN      
        1 atcatcagat cattgaaaat caactggata cagaaacatt aactgttagt tatgaattta
       61 tcctctgtca acatcttatc gcaaataccg agttcagcta tttggcactc cctgaaaatt
      121 ataactgttt gtttttacca aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa
      181 acagcaaagc tattggcagg ctacttgctg ctggtggcgt atacaatggg aacattgaag
      241 gattcagaga tactgcggaa aaactgggtg gagatgcaat aaaaggctat gatcaaatac
      301 taaatgaaaa aacagcgggc atagcgatag caacagcatc tattctttta acaaagcgtt
      361 ctaatgttga tacatataca gaaataaata gttacttagg caaacttaga ggtcaacaaa
//
LOCUS       DAYPHN010000434          391 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000434 DAYPHN010000000
VERSION     DAYPHN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.443"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            1..391
                     /locus_tag="ACG0BW_005013"
                     /pseudo
     CDS             1..391
                     /locus_tag="ACG0BW_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366955.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    <1..72
                     /locus_tag="ACG0BW_005013"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT           91 a           96 c          125 g           79 t
ORIGIN      
        1 atgccactgc tggataagct gcgtgagcag tacggggtcg gaccggtatg cagtgaacag
       61 catattgccc cgtcaacgta ttaccattgt cagcagcagc ggcatcatcc tgataaacgc
      121 agtgccccgt gcgcagcgcg atgactggct gaagagagag atacagcgcg tatacgatga
      181 aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg aaggaatcag
      241 ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg ggacttgccg gtgttctccg
      301 gggtaaaaag gtccgcacta ccgtcagccg gaaaaccgtt gctgcaggtg accgcgtaaa
      361 ccgtcagttc gtggcggaac gtcctgacca g
//
LOCUS       DAYPHN010000435          390 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000435 DAYPHN010000000
VERSION     DAYPHN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.444"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(1..269)
                     /locus_tag="ACG0BW_005014"
                     /pseudo
     CDS             complement(1..269)
                     /locus_tag="ACG0BW_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          108 a           86 c           70 g          126 t
ORIGIN      
        1 ttcattacca cgtccagacg ccagtgcagc ttattctcca cgtgccagtg atttcgtatc
       61 gctgtggcga acttttttgc ggttaaatca gcagaactga tataatatct gaccgtcatt
      121 tctggctctt tcttttgttc tgctattatt gaccaaaagg agactgccac gcataatttc
      181 ttctgacctt tccattcaaa cgtgaaatca ataagttcat cagggacatc gcaaacaata
      241 tgaagatgga tttcttctct gccgtgacta aaatgcgaat tatttagtac aaaaaagcgg
      301 cgcaccttgt tctttcacag attcctaata tggcaacttt aagttatcgc cgtctatact
      361 tatagaaatt tcatcagggg tgacgtaaat
//
LOCUS       DAYPHN010000436          386 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000436 DAYPHN010000000
VERSION     DAYPHN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.445"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..272)
                     /locus_tag="ACG0BW_005015"
     CDS             complement(<1..272)
                     /locus_tag="ACG0BW_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HFK7261819.1"
                     /translation="MNKLVLVGHPGSKYQIVEHFLKEIGMNSPNYSTSNKISPEYITA
                     SLCQFYQTPEVNDVVDEREFSAVQVSTMWDSMVLELMMNNLNNKLWG"
BASE COUNT          114 a           64 c           61 g          147 t
ORIGIN      
        1 ccccaaagtt tgttatttag attgttcatc attagttcaa gaaccatgct atcccacatg
       61 gttgagactt gaacagctga gaattctctc tcatctacta catcattaac ttctggtgtt
      121 tgataaaatt gacataatga agcggtgata tattctgggg aaattttatt acttgtagaa
      181 taatttggtg agttcatgcc aatttctttc aaaaaatgtt caactatctg atactttgag
      241 ccaggatgtc cgactagcac taatttattc atcatatttc ctctttatta tctacaagat
      301 tactttattt taaacactgt cgtttatcgt aatggtgata ttgcagacat atatttcaaa
      361 tctgtaatgt aatttaggtg gcgtta
//
LOCUS       DAYPHN010000437          385 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000437 DAYPHN010000000
VERSION     DAYPHN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.446"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>385
                     /locus_tag="ACG0BW_005016"
     CDS             <1..>385
                     /locus_tag="ACG0BW_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFK7261820.1"
                     /translation="SATNKTSLSFMIEYGNFCGLFLGDAHEEDIYSYMANHCANRKIS
                     FNVVKLAHHGSEKNTSSRLLKLIGTTEYIICANKTKHNHPNNLTLARILIHEQEPKIH
                     MSSSNQEIINLLNQINLLGFGITAT"
BASE COUNT          148 a           61 c           61 g          115 t
ORIGIN      
        1 catcggcaac taataaaacc agtttatcat ttatgattga gtatgggaat ttttgtggat
       61 tattccttgg tgatgcacac gaagaagaca tctactcata tatggctaat cattgtgcca
      121 atagaaaaat atcatttaac gttgttaagt tagcacatca tggtagtgaa aaaaacacga
      181 gttcacggtt attgaagtta attggtacaa cagaatatat catatgtgcg aataaaacca
      241 agcataatca tccaaataat ttaactcttg caagaatttt aatacatgaa caagaaccta
      301 aaattcatat gagtagcagc aatcaggaga taataaattt actcaatcaa ataaatttat
      361 taggcttcgg cattactgca accca
//
LOCUS       DAYPHN010000438          378 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000438 DAYPHN010000000
VERSION     DAYPHN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.447"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>378
                     /locus_tag="ACG0BW_005017"
     CDS             <1..>378
                     /locus_tag="ACG0BW_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFK7261821.1"
                     /translation="NSKDIENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEF
                     VTKVDNKAEVFDKIKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNE
                     KKQVSSERLTLDNYIDDLHFDNS"
BASE COUNT          135 a           56 c           72 g          115 t
ORIGIN      
        1 taaactctaa ggatattgaa aatattcttg atggcaaagg ggatttattt aaaaaacgta
       61 cgctgtggga gtttgtccgc gatctttttc ccggctctca tattaaagaa gtcaaaggct
      121 taatttacga atttgtcacg aaagttgata acaaagccga ggtgttcgat aagattaaat
      181 ctctggcaaa aaaagaacaa caatggcgat ttagtactaa gacagacttt accacaaatg
      241 aaaataatga agttattgtg agtagatcct ttaatctcta cacgggggct acttcgaatg
      301 ataacgaaaa aaaacaggtt tcttcagaga gattaacgct tgataattat attgatgatt
      361 tacattttga taattctc
//
LOCUS       DAYPHN010000439          374 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000439 DAYPHN010000000
VERSION     DAYPHN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.448"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            121..>374
                     /gene="yqeK"
                     /locus_tag="ACG0BW_005018"
     CDS             121..>374
                     /gene="yqeK"
                     /locus_tag="ACG0BW_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFK7261822.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRYVNVNSVSKVNVKSKVKNSYISRSVNDEFSLTD"
BASE COUNT          136 a           48 c           78 g          112 t
ORIGIN      
        1 gagacgttac gtagaatgac tatatttgtt atcggcacat taggggtatt aaatttagta
       61 ttgatggaag gtgattattg aacagtaaca tcgagtcacg gtaaaaatgt gaggtatata
      121 atggacattg agttttcgca gattcatgaa atggtctata tgcatgatat tgtaaattcg
      181 gactcgaaaa agaaaccaag gattccgtta aaaaaattcc ttaatgctga gaaggttctc
      241 acgcaaacaa catcatgggc actaaattca agatatgtga atgttaattc tgtcagtaaa
      301 gtcaacgtaa agagtaaagt taaaaacagt tatatctcac gatctgtcaa tgatgagttt
      361 agtttaacag atga
//
LOCUS       DAYPHN010000440          371 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000440 DAYPHN010000000
VERSION     DAYPHN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.449"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>371
                     /locus_tag="ACG0BW_005019"
     CDS             <1..>371
                     /locus_tag="ACG0BW_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HFK7261823.1"
                     /translation="LIKKYGKKYADNYGWASYIFPNHNKVGFGAIEKDVQLEHMRPYY
                     KWASQNVHTGSKAMRNRLGLCETGDDILLVGQSDSGMADPAHATAISLMQITVTLLFL
                     KPTIDHAVISKIIQDYSDEIG"
BASE COUNT          121 a           58 c           80 g          112 t
ORIGIN      
        1 attaataaaa aaatatggca aaaaatatgc agataattat ggctgggcat cttatatttt
       61 ccctaaccat aataaagtgg ggtttggagc tattgagaaa gatgttcagt tagaacacat
      121 gcgcccttat tataagtggg cgagtcagaa tgtacacacc ggttccaagg caatgagaaa
      181 taggcttggt ctctgtgaaa ctggtgatga tatattattg gtcgggcaaa gtgattcagg
      241 aatggctgat ccagcccatg ctactgctat tagtttgatg cagattactg tgacactttt
      301 atttttaaaa ccaaccattg atcatgccgt catttcaaaa ataatacagg attattctga
      361 tgagattgga a
//
LOCUS       DAYPHN010000441          368 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000441 DAYPHN010000000
VERSION     DAYPHN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.450"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            57..>368
                     /locus_tag="ACG0BW_005020"
     CDS             57..>368
                     /locus_tag="ACG0BW_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFK7261824.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QY"
BASE COUNT           88 a          103 c           97 g           80 t
ORIGIN      
        1 ttcttcactt tccgctgatt cggtgccaga ctgaaatcag cctataggag gaaatgatgg
       61 tacgtatcta tacgttgaca cttgcgccct ctctcgatag cgcaacaatt accccgcaaa
      121 tttatcccga aggaaagctg cgctgtaccg caccagtatt cgaacccgga ggcggcggca
      181 tcaacgtcgc ccgcgccatt gcccatcttg gaggcagtgc cacagcgatc ttcccggcgg
      241 gtggtgcgac cggcgaacat ctggtttcac tgttggcaga tgaaaatgtc cccgtcgcta
      301 ctgtagaagc caaagactgg acccggcaga atttacacgt acatgtggaa gcaagcggtg
      361 agcagtat
//
LOCUS       DAYPHN010000442          361 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000442 DAYPHN010000000
VERSION     DAYPHN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.451"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>361
                     /locus_tag="ACG0BW_005021"
     CDS             <1..>361
                     /locus_tag="ACG0BW_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFK7261825.1"
                     /translation="ADVRTAKIASLEVTRDNSVADGAMANTLRVKVTDAFGNALNGQT
                     VSVMADNGATVAPTVITEPDGTVEISVTSQTAGVSAVTATINSSSQSQNVTFIADVST
                     AKIADLVVIKDGSEADGS"
BASE COUNT           86 a           89 c          126 g           60 t
ORIGIN      
        1 gccgatgtca ggacggcgaa gatcgcgagt ctggaggtta cccgggataa ctcagtggct
       61 gacggcgcaa tggcgaacac gctgcgggtg aaggtcaccg atgcgttcgg gaatgcgctg
      121 aatggacaga cggtcagcgt gatggcggac aacggcgcaa cggttgcccc gacggtgatc
      181 accgagccgg acggcacggt ggaaatcagt gtcaccagcc agacggcggg agtcagtgca
      241 gtcactgcaa ccatcaacag cagtagtcag agtcagaacg tgacatttat cgccgatgtc
      301 agcacggcga agatcgctga tctggtggtc attaaggatg gttcagaggc ggacggttcg
      361 a
//
LOCUS       DAYPHN010000443          359 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000443 DAYPHN010000000
VERSION     DAYPHN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.452"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            12..284
                     /locus_tag="ACG0BW_005022"
     CDS             12..284
                     /locus_tag="ACG0BW_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFK7261826.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
BASE COUNT          126 a           61 c           55 g          117 t
ORIGIN      
        1 ttagataata tatgactccc tccccctatc caagaaataa ctttaataca gagctaattc
       61 aatcatgtat gaatggagaa cacttttcac ttttaattga aatatcacca atcagaagta
      121 aaaaaaacat catggctctt aaggagtatt tggttgatgg ttattcaaag caagaaagtt
      181 gtgaaaggaa caatgtttcc attagttatt tttgcctatg cctaaaaaaa ctaaactata
      241 cacacagtat tgttagtgct cttaaatatt tctaccgtga gtaaactgta actgaagtct
      301 tgattcttac gcttagacac tgctaagccg ttatggcata gctgataatt tatattgca
//
LOCUS       DAYPHN010000444          355 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000444 DAYPHN010000000
VERSION     DAYPHN010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.453"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>355)
                     /locus_tag="ACG0BW_005023"
     CDS             complement(<1..>355)
                     /locus_tag="ACG0BW_005023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261827.1"
                     /translation="ALSADFPAAVALAEACPALVDAALADDAAAVFESAAAEALCSAA
                     VSEDLAFVSDVFAAFAEFAAAVAEEAALLALEDAFVSDDFAASFETAASRAEEAASDA
                     FVVAVDAEVAADCCDA"
BASE COUNT           95 a          115 c          100 g           45 t
ORIGIN      
        1 gcgtcacaac aatcagccgc cacttctgca tccaccgcga ctacgaaagc gtcagaagca
       61 gcctcctcag cccgggatgc ggcggtttca aaagaggcgg caaaatcatc agaaacgaac
      121 gcatcctcga gcgccagtag cgcagcttcc tcggcaacgg cggcagcaaa ttccgcgaag
      181 gcggcaaaaa cgtccgagac gaacgctaag tcttctgaga cggcagcgga acagagtgcc
      241 tccgcagcag cagactcaaa aacagcggct gcatcatctg ccagcgccgc gtcaacaagt
      301 gccgggcagg cctcagccag tgccaccgct gccggaaagt cggcagaaag tgctg
//
LOCUS       DAYPHN010000445          353 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000445 DAYPHN010000000
VERSION     DAYPHN010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.454"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>353)
                     /locus_tag="ACG0BW_005024"
     CDS             complement(<1..>353)
                     /locus_tag="ACG0BW_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261828.1"
                     /translation="KNRMKVFAGTNFIDFNMTGQDLSGFVLTLSRFYFEDLLNINFTD
                     ANLGDTIFSHKEHPTPKLYKDEQYLDKQIEGLFSTLLTINDNFLRAKAEIASTIIKFL
                     EARITNLSYNDILKY"
BASE COUNT          109 a           49 c           63 g          132 t
ORIGIN      
        1 atattttaat atgtcattat atgaaagatt ggttatcctg gcctctaaga atttaattat
       61 tgtcgaagca atttccgctt ttgctcgtaa aaagttgtca ttaatggtta ataatgttga
      121 aaataaacct tcgatttgtt tgtcaagata ttgttcgtct ttatataatt ttggggtagg
      181 gtgttcttta tgtgaaaaaa tagtgtcacc taaatttgcg tcagtgaaat ttatattaag
      241 tagatcttcg aaataaaacc ttgacaaggt tagaacgaac ccggacagat cctgtcctgt
      301 catattgaaa tcaataaagt tggttccggc aaagactttc atcctgtttt tat
//
LOCUS       DAYPHN010000446          352 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000446 DAYPHN010000000
VERSION     DAYPHN010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.455"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            53..>352
                     /locus_tag="ACG0BW_005025"
     CDS             53..>352
                     /locus_tag="ACG0BW_005025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261829.1"
                     /translation="MNYKTPCSPIINDYFEKLPLHVRNYFEELLKTANDRVTHFDVYI
                     PRLIGCSIVQHYEDVPSEKNRLNDRNDVIAMAEDYIEKRKSNQLDYILEDIYFKSD"
BASE COUNT          120 a           62 c           64 g          106 t
ORIGIN      
        1 cgttaaaagg gactggatgt ttcgacaccc aaagctcgta ccgtcggttc ttatgaatta
       61 taaaactcct tgttctccta taattaatga ttactttgag aagttgcctt tacatgtaag
      121 aaactacttt gaggaactac ttaaaaccgc gaatgataga gttactcatt ttgacgttta
      181 catcccacgc cttattggtt gttcaatcgt gcaacattac gaagatgtgc cttccgaaaa
      241 aaacaggtta aatgacagga atgatgtaat agctatggct gaagattaca tcgagaaaag
      301 aaaatctaat caactagatt acatacttga agatatatac tttaagagtg ac
//
LOCUS       DAYPHN010000447          346 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000447 DAYPHN010000000
VERSION     DAYPHN010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.456"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>346
                     /locus_tag="ACG0BW_005026"
     CDS             <1..>346
                     /locus_tag="ACG0BW_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFK7261830.1"
                     /translation="YSKKKINFIFCTSTLLEGVNLPTKNVFIYPFPKKTVNDEKKCRL
                     DFWNLAGRAGRYRSELTGNIVCLNTADNNWSNADAKISLGNNVIIEDEINSTLLRHQK
                     ILNYFDGKVKSPT"
BASE COUNT          133 a           52 c           59 g          102 t
ORIGIN      
        1 atattccaaa aagaaaataa attttatctt ttgcacatca actttacttg aaggggtaaa
       61 cttacccaca aagaatgttt ttatttatcc attccctaaa aagactgtta acgatgaaaa
      121 aaaatgccga ttagattttt ggaatctggc ggggcgagct ggaagataca gaagtgaatt
      181 gactggtaac atagtatgcc tgaataccgc tgataataac tggagcaatg cagatgctaa
      241 aattagccta ggtaataatg ttattataga ggatgaaata aactcaacat tactacgcca
      301 tcaaaaaata ttaaattatt ttgatggtaa agttaaatct ccaact
//
LOCUS       DAYPHN010000448          343 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000448 DAYPHN010000000
VERSION     DAYPHN010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.457"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            229..297
                     /locus_tag="ACG0BW_005027"
                     /pseudo
     CDS             229..297
                     /locus_tag="ACG0BW_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          133 a           52 c           61 g           97 t
ORIGIN      
        1 ttgttattcc tttcatttat atcgttaccc gcacagaaaa tggaaaagaa aacaaaaaac
       61 cagggtaaaa gccctatatc ggaacataaa aaatgagcga ttttttacag tattcatagg
      121 tggtttagtc ataatcgtag caaaccaaca cccaagtatg ctttcttaag acaaatcggc
      181 aatgtgttgt gctcgggcga ataaatattg tgttcaggag aaatgattat ggctatatca
      241 tcatatagta cagttacaga taaatttgct actctgacta aaatgcaaga tgaaaatctg
      301 aatgaaaaaa atagaacgca aaaatattct ggaaaaattg gtt
//
LOCUS       DAYPHN010000449          329 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000449 DAYPHN010000000
VERSION     DAYPHN010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.458"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..261)
                     /locus_tag="ACG0BW_005028"
     CDS             complement(<1..261)
                     /locus_tag="ACG0BW_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFK7261831.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDK"
BASE COUNT           95 a           64 c           68 g          102 t
ORIGIN      
        1 tttgtcgatc cagtttttgt taatggataa atccatcaac ttatggacat tttcgtcgtc
       61 ccaggtctgg cagaaagtgg ccaggttctg acgagcgttg ccatcaagat ataattcgtc
      121 attgataatc tggaatgcga catcatcgcg catttcgtgc agcggaaatc gttttgattc
      181 tgcgatagtg gaaatagact tcgcaccaaa acgtgaatcg agtagttccg accttaaatc
      241 cgttacttgc ttcttatcca ttttaaactc cttaaaatga ttggatcgca ttaaaaaagt
      301 aggatttatc gataaagtaa gcaagtgca
//
LOCUS       DAYPHN010000450          328 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000450 DAYPHN010000000
VERSION     DAYPHN010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.459"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>328)
                     /locus_tag="ACG0BW_005029"
     CDS             complement(<1..>328)
                     /locus_tag="ACG0BW_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261832.1"
                     /translation="KNNRIFANITFTNNSSTTYFFSKYYLPMKVKDGKGDVYDAICKK
                     PFRINSGNITLDYLGGSCPFEINTNPERWNTIKPGRSFLLSITLNDMYAFIPGDNYYF
                     IKSSGY"
BASE COUNT          108 a           52 c           50 g          118 t
ORIGIN      
        1 ttatagccag atgattttat aaaataataa ttatctcctg gtataaaggc atacatatca
       61 tttaaggtta tactcaacaa aaaactacga cctggtttaa tagtattcca ccgttcagga
      121 ttcgtattaa tttcaaaagg acatgaacca cctaagtagt ccaacgtgat attcccagag
      181 ttgattctga atggtttttt gcatatcgca tcatatacat ctccttttcc atcttttact
      241 ttcataggta ggtaatattt agaaaaaaaa tacgtggtag agctattgtt agtaaaggtt
      301 atgttagcaa aaatcctgtt gttttttt
//
LOCUS       DAYPHN010000451          324 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000451 DAYPHN010000000
VERSION     DAYPHN010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.460"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(173..>324)
                     /locus_tag="ACG0BW_005030"
     CDS             complement(173..>324)
                     /locus_tag="ACG0BW_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFK7261833.1"
                     /translation="AELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLAAVLA
                     GCGLS"
BASE COUNT           94 a           81 c           52 g           97 t
ORIGIN      
        1 acattaattc ctaaatattt gaaaatttcc tgacagtttc tacagttcgg aaaagcactt
       61 ccatcaccag tatgaactgt agccatttcc aaatcatgca acgatgaatt atctttgaga
      121 gctttattca ccgcatctat ttcagcacaa ttattaacag cccaaggcaa gattacgaaa
      181 gcccgcaccc cgcaaggact gccgccaggt agtttctgtc catggctgct tttcgcatct
      241 tacgtcttaa ccctgccttg aataccttat cattcgttaa aatattaata gcgatgtgcc
      301 gtatccctga aaataattct gcgg
//
LOCUS       DAYPHN010000452          322 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000452 DAYPHN010000000
VERSION     DAYPHN010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.461"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..242)
                     /locus_tag="ACG0BW_005031"
     CDS             complement(<1..242)
                     /locus_tag="ACG0BW_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFK7261834.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           92 a           67 c           73 g           90 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tccaggctta at
//
LOCUS       DAYPHN010000453          321 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000453 DAYPHN010000000
VERSION     DAYPHN010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.462"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(74..319)
                     /gene="dinI"
                     /locus_tag="ACG0BW_005032"
     CDS             complement(74..319)
                     /gene="dinI"
                     /locus_tag="ACG0BW_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFK7261835.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT           94 a           75 c           68 g           84 t
ORIGIN      
        1 aattttgtac tcaaaatttg taagtaaaat aaaaaagccg gggcgacccg gcaaaaacaa
       61 aaatcactgc atattattcg ctgacaaacc agtcatcggc gctttcccac gtttcctgga
      121 gaatttcgct aatgcgctgt ttatcttctt ttgttgcgcc aataaccgat aaattattcg
      181 ctgcggcata acgtaccgat acgtggcctt cattatcagg aaacgcatac tgaatacggc
      241 gggaaagttc gccagccagg gcgtcaatag ccccagctgg caatggagaa gttttcgcta
      301 tggtgacttc aattcgcata a
//
LOCUS       DAYPHN010000454          320 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000454 DAYPHN010000000
VERSION     DAYPHN010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.463"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..290)
                     /gene="tssD"
                     /locus_tag="ACG0BW_005033"
     CDS             complement(<1..290)
                     /gene="tssD"
                     /locus_tag="ACG0BW_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFK7261836.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNSITNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMEFDFYRINRFG"
BASE COUNT          101 a           63 c           49 g          107 t
ORIGIN      
        1 ctaccaaatc tatttattcg atagaagtca aattccataa ataattgttc attattgtta
       61 atggaattaa taaataatgg agtgctttta tcaattaatt tacaaaatgt tataccatgg
      121 aactgtgaac caagccccgt attagtaata ctatttaaga gtgagaatgt aaatatttca
      181 tcctcatgcc cgctctgcca gcgattacct gtagactcag aagtcccaca acctgcggag
      241 atgcttcctt gttgttctcc cgttactgtc aggtaaacaa tattactcat ccatgaaaag
      301 tccttttatg gcatgctatt
//
LOCUS       DAYPHN010000455          317 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000455 DAYPHN010000000
VERSION     DAYPHN010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.464"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>317
                     /locus_tag="ACG0BW_005034"
     CDS             <1..>317
                     /locus_tag="ACG0BW_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFK7261837.1"
                     /translation="TNINTACVKNNASYQLNNALPNKETISSNFCERLAQWGNKSLNN
                     GEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLEKNCLTCLDFTDNA
                     SLV"
BASE COUNT          107 a           63 c           56 g           91 t
ORIGIN      
        1 gaccaatatt aatacagctt gtgtaaaaaa taatgccagt tatcaattaa ataacgcatt
       61 acctaacaaa gaaaccatct ccagcaattt ttgtgaacga ctggcacaat ggggtaataa
      121 gtcgcttaat aatggtgaag aaagagctat tgccgtagag cgaattaaag aagcttacaa
      181 ttcgaacatg gcatctcttg atttgtcata tcttgatttg agtgagttac cgcctatccc
      241 ctcgacagtt aacacgttaa atctggaaaa aaattgtctc acttgtcttg actttactga
      301 caatgccagc ctcgtca
//
LOCUS       DAYPHN010000456          304 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000456 DAYPHN010000000
VERSION     DAYPHN010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.465"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>304)
                     /locus_tag="ACG0BW_005035"
     CDS             complement(<1..>304)
                     /locus_tag="ACG0BW_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261838.1"
                     /translation="IVITKVDGVSNYPLRIGKIEKEKYYTYGYKSKCDKPGRILLNEP
                     DLLDGICYFNICSDATPELIEKSEEYYGISGSLVFDSPEKDVLTAHAVITTNETNND"
BASE COUNT           96 a           59 c           36 g          113 t
ORIGIN      
        1 gtcattattg gtttcattag ttgttattac tgcatgtgcg gttaaaacat ctttttctgg
       61 agaatcaaaa actaatgaac ctgaaattcc ataatattct tcacttttct caatcaactc
      121 aggtgttgca tcggaacata tattgaaata acaaatccca tctaataaat ctggctcatt
      181 taataaaatt ctgccaggct tatcacattt actcttgtaa ccataagtat aatacttttc
      241 tttttctatc tttcctatcc ttaaaggata attagatacc ccatccactt tggtgatgac
      301 aata
//
LOCUS       DAYPHN010000457          299 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000457 DAYPHN010000000
VERSION     DAYPHN010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.466"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..242)
                     /locus_tag="ACG0BW_005036"
     CDS             complement(<1..242)
                     /locus_tag="ACG0BW_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261839.1"
                     /translation="MPFLIFIIILLLTVIFWDWVVLNGQTVGTLATAFAFIATAWNAY
                     EARKSAKAAFSALQLTTESLFEMRKSAFKQWFDSLL"
BASE COUNT          107 a           54 c           46 g           92 t
ORIGIN      
        1 tttaataagg aatcaaacca ttgtttaaat gcactctttc gcatttcgaa caatgattca
       61 gttgttaatt gaagggctga aaaagctgct tttgcacttt ttctcgcttc gtaggcattc
      121 catgctgttg ctataaaagc aaatgctgta gccaatgttc caacagtttg accatttaaa
      181 acaacccaat cccaaaaaat gacggtgagc agtagtataa taataaaaat taaaaaaggc
      241 atgtatgtat ttcactctac taactataaa atattaggct aatgttaacc aaacaatgc
//
LOCUS       DAYPHN010000458          297 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000458 DAYPHN010000000
VERSION     DAYPHN010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.467"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>297)
                     /locus_tag="ACG0BW_005037"
     CDS             complement(<1..>297)
                     /locus_tag="ACG0BW_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFK7261840.1"
                     /translation="RLQQLEDACRDIIKINMWYELPDYVFFSISDTIDGTSDEFAINV
                     YKNIDKSLLEILLHPSVLLSVVESLQSNTIAEYIVKLSEIEMNYDDLLISDVKS"
BASE COUNT          103 a           54 c           40 g          100 t
ORIGIN      
        1 atgatttaac atccgatatt agcaagtcgt cataattcat ctctatttca gacaatttca
       61 cgatatactc agctatagta ttactttgta aggactcaac aactgacagt aaaacagatg
      121 gatgcaataa tatttcaaga agacttttat cgatattttt atacacattt attgcgaatt
      181 catcactcgt cccatcgatg gtgtcagata ttgaaaaaaa tacataatcg gggagttcat
      241 accacatgtt aattttaata atatcacgac acgcatcttc taactgttgt aatctat
//
LOCUS       DAYPHN010000459          296 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.468, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000459 DAYPHN010000000
VERSION     DAYPHN010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.468"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>296)
                     /locus_tag="ACG0BW_005038"
     rRNA            complement(<1..>296)
                     /locus_tag="ACG0BW_005038"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           69 a           82 c           70 g           75 t
ORIGIN      
        1 aaacattaaa gggtggtatt tcaaggtcgg ctccatgcag actggcgtcc acacttcaaa
       61 gcctcccacc tatcctacac atcaaggctc aatgttcagt gtcaagctat agtaaaggtt
      121 cacggggtct ttccgtcttg ccgcgggtac actgcatctt cacagcgagt tcaatttcac
      181 tgagtctcgg gtggagacag cctggccatc attacgccat tcgtgcaggt cggaacttac
      241 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt taccgg
//
LOCUS       DAYPHN010000460          296 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000460 DAYPHN010000000
VERSION     DAYPHN010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.469"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
BASE COUNT           70 a           52 c           71 g          103 t
ORIGIN      
        1 gtttttgtta tttataaaaa tgtcatcctg tccataaaat attgtactca gcattcagtg
       61 caggaactgt agtcttcttt attttattct tcttcaggag gggggggtga ttcctgaagg
      121 cgttattccc ggtgttctgt ttatgattct tcccggcagg aaaccggtat ctgcaatacc
      181 cgggatgatg ctttttgttc gtacgtggtt gtgtaaaatt tgaatagtgg atgtgctccg
      241 aagggaagga ggggacacac agattcattt tgtctttctt cagcagcaaa atacac
//
LOCUS       DAYPHN010000461          293 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000461 DAYPHN010000000
VERSION     DAYPHN010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.470"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            89..202
                     /locus_tag="ACG0BW_005039"
                     /pseudo
     CDS             89..202
                     /locus_tag="ACG0BW_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          111 a           51 c           48 g           83 t
ORIGIN      
        1 aaaggtaaca aaaatcattg taaaccatgc catcttttta gagattacca gcagaaaatt
       61 ccttgttcga aacttagcaa tgcaagctat ggaaaaactt acaacggaac tacactcctt
      121 aagtgagatg gaccgaagac atgtaatctc tattttagcc gaaatagcta atggctatga
      181 tgattttagt gacatgttga tttagttaga acttcacccg aatcataaga ttctgatatc
      241 ttaaataaga aaatcccgtt cgacgttctg aaaaagaaat cacaaaatat tag
//
LOCUS       DAYPHN010000462          293 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000462 DAYPHN010000000
VERSION     DAYPHN010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.471"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..192
                     /locus_tag="ACG0BW_005040"
     CDS             <1..192
                     /locus_tag="ACG0BW_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HFK7261841.1"
                     /translation="QKQYFKSIFLGTITCLSIVYPACKYYGATGAIVSLIVAEIFVGM
                     GMLKQFIKVNKTVCRPHKL"
BASE COUNT           97 a           48 c           52 g           96 t
ORIGIN      
        1 caaaaacagt atttcaaatc tattttccta ggaactatta cttgtttaag catagtttat
       61 ccagcatgta aatattatgg agcaacgggt gcgattgtga gtcttattgt agctgaaatt
      121 ttcgttggca tgggaatgct taaacaattc attaaagtaa ataaaaccgt atgtaggcct
      181 cataaattat gaatatctcg gtaataatat ctgtttggaa acgcccagtt caattagaat
      241 tgattctctc tgagctcgat tctcaggcta aagacaatag tctacaccta gaa
//
LOCUS       DAYPHN010000463          285 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.472, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000463 DAYPHN010000000
VERSION     DAYPHN010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.472"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            65..>285
                     /locus_tag="ACG0BW_005041"
     CDS             65..>285
                     /locus_tag="ACG0BW_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFK7261842.1"
                     /translation="MVKIHILDAGHGDCLLVDCDGVKLLIDAGPSTFRYRKKISAKLA
                     ELLNGESVDIAFVTHNDDDHIGGFKYLIE"
BASE COUNT           93 a           44 c           57 g           91 t
ORIGIN      
        1 acaggaggac tcattgtgat ttatataata aaataacctt atacatcttt ctggaaacca
       61 agtaatggtt aaaattcaca ttttagatgc cggacatggt gattgcttgt tagtagactg
      121 cgatggtgtc aaattgttaa ttgacgctgg cccaagtaca tttaggtata gaaaaaaaat
      181 ttcagccaag ttagctgaac ttctgaatgg cgagtctgtt gatatcgctt ttgtaactca
      241 caatgatgat gaccatatcg gtggtttcaa atacctaatt gaaaa
//
LOCUS       DAYPHN010000464          283 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000464 DAYPHN010000000
VERSION     DAYPHN010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.473"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>283
                     /gene="tnpA"
                     /locus_tag="ACG0BW_005042"
     CDS             <1..>283
                     /gene="tnpA"
                     /locus_tag="ACG0BW_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFK7261843.1"
                     /translation="TEKLRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSL
                     VNSLKGVSGRLLRRDRPDIAVRYYYKGVLWSPGYFASSCGGAPISVIRQY"
BASE COUNT           78 a           63 c           66 g           76 t
ORIGIN      
        1 acagaaaaac tacgcactta cttttcaaat gtatgtgctg attttgaagc tgaactggtt
       61 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg
      121 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
      181 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
      241 agtagctgcg gaggtgcgcc aatatccgtc atccgccaat aca
//
LOCUS       DAYPHN010000465          283 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000465 DAYPHN010000000
VERSION     DAYPHN010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.474"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..261
                     /locus_tag="ACG0BW_005043"
     CDS             <1..261
                     /locus_tag="ACG0BW_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C551 peroxidase"
                     /protein_id="HFK7261844.1"
                     /translation="TAADIGRMNVTKEERDKLRQKVPGLRNVALTAPYFHRGDVPTLD
                     GAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
BASE COUNT           76 a           59 c           83 g           65 t
ORIGIN      
        1 acggcggcgg atattggtcg tatgaatgtt actaaagaag agcgcgataa attgcgtcag
       61 aaagtacccg gtttacgtaa cgttgcacta acggcaccgt acttccatcg cggtgatgtg
      121 ccgacgctgg acggggcggt gaaactgatg ttgcgctatc aggtaggcaa agagctgccg
      181 caggaggatg tggatgatat cgtagctttc ctgcacagtc tgaacggggt ttacacgccg
      241 tatatgcagg ataaacaata attaatttaa tcgcccgaac agg
//
LOCUS       DAYPHN010000466          282 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.475, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000466 DAYPHN010000000
VERSION     DAYPHN010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.475"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>282
                     /locus_tag="ACG0BW_005044"
     rRNA            <1..>282
                     /locus_tag="ACG0BW_005044"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           81 a           67 c           85 g           49 t
ORIGIN      
        1 tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga
       61 atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc
      121 agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag
      181 acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg
      241 tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tg
//
LOCUS       DAYPHN010000467          271 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000467 DAYPHN010000000
VERSION     DAYPHN010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.476"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(89..271)
                     /locus_tag="ACG0BW_005045"
                     /pseudo
     CDS             complement(89..271)
                     /locus_tag="ACG0BW_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           74 a           63 c           54 g           80 t
ORIGIN      
        1 acaatagagt ttatataatt atatagagtg tgattacaat ctccggcaat gacactgtta
       61 gtgcaacagt aatcaggcct ccctcttagt attgcttacg ctgaaagaat aactggcagc
      121 ctgccacacc atgttgtctg gcaacgagga agaccgccat tttcggttca aggctctgct
      181 gaaaaatagc gattttcctg tgtggcacgc tgtctgcgtt tctctgcttc taagacacca
      241 gttatctatt ctccaactac tagtctaaaa g
//
LOCUS       DAYPHN010000468          266 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000468 DAYPHN010000000
VERSION     DAYPHN010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.477"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(57..>266)
                     /locus_tag="ACG0BW_005046"
     CDS             complement(57..>266)
                     /locus_tag="ACG0BW_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261845.1"
                     /translation="VFFTDQFIAEFSPQEFVDNCEQYLISNHCFSPDKVNEICEHARH
                     YLVERMCEIDSLAMNNSDLTTPEDL"
BASE COUNT           97 a           47 c           40 g           82 t
ORIGIN      
        1 gcgtttaaaa tattagaatt agattatatt caagtcatta agccggagac agtgatctat
       61 aagtcctctg gggtcgtcaa atcagaatta ttcatagcca aagaatctat ttcacacatt
      121 ctttcaacta aataatgtct ggcatgttca cagatttcat taaccttgtc aggggaaaaa
      181 cagtgattgc taattaaata ttgctcacaa ttatccacaa attcctgcgg actaaattca
      241 gcaataaatt gatctgtaaa aaaaac
//
LOCUS       DAYPHN010000469          263 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000469 DAYPHN010000000
VERSION     DAYPHN010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.478"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>263
                     /locus_tag="ACG0BW_005047"
     CDS             <1..>263
                     /locus_tag="ACG0BW_005047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ERCC4 helicase"
                     /protein_id="HFK7261846.1"
                     /translation="IMNDAINYICNRIKNSDGFSYCYEKLLISISKNLFNPTSSNPIT
                     QREYQQLLKFSDFLSNSEKEEDRNLALKIISAIYDLYKEDHSC"
BASE COUNT          106 a           40 c           35 g           82 t
ORIGIN      
        1 aattatgaat gatgctatta attatatctg caatagaata aaaaatagtg acggatttag
       61 ttactgttat gaaaaactat tgatatctat ttcaaagaat ctattcaatc caactagcag
      121 caaccccatt actcagcgtg aatatcaaca attgctaaaa ttcagcgatt ttttatctaa
      181 ttccgaaaaa gaagaagatc gaaacctagc gttaaaaata atatctgcaa tatatgatct
      241 ttataaagaa gatcatagtt gcc
//
LOCUS       DAYPHN010000470          261 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.479, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000470 DAYPHN010000000
VERSION     DAYPHN010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.479"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
BASE COUNT          112 a           48 c           28 g           73 t
ORIGIN      
        1 atcataaaat tacgcactaa atacataata ccaaaaaatg caggaataaa tgccaaatat
       61 aagaaaggta ttatcagcga caatctaaaa aaaacaacaa aaaataataa aaaagccatc
      121 aatgcattat acttcattgt tttttacctt ccatacaaat gtcttaataa atgaagatag
      181 catcatctat atataccagt tcttaagcaa atcatctgtt tttgaggtgc cacgctctcc
      241 aataccatgt atatatgccg a
//
LOCUS       DAYPHN010000471          258 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000471 DAYPHN010000000
VERSION     DAYPHN010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.480"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>258)
                     /locus_tag="ACG0BW_005048"
     CDS             complement(<1..>258)
                     /locus_tag="ACG0BW_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFK7261847.1"
                     /translation="LSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVID
                     VDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLQQRLTEK"
BASE COUNT           92 a           60 c           28 g           78 t
ORIGIN      
        1 tttctccgtt agtctttgtt gtaaccattc ccgtaaatta atatacatta caccagagtt
       61 aaaatagcta ccgttaaaat cctcattaca taaacgctca gcgcttttac tttgcatagc
      121 atctacatca attaccacag ccccatattc atctttaaac tctaatgcta ttaactcgtt
      181 caatgaaccc ttacagacga tatcagcatc aagatataat aatgaatcca atcgcgcaga
      241 aaaataatca aatgaaag
//
LOCUS       DAYPHN010000472          257 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000472 DAYPHN010000000
VERSION     DAYPHN010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.481"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>257
                     /locus_tag="ACG0BW_005049"
     CDS             <1..>257
                     /locus_tag="ACG0BW_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFK7261848.1"
                     /translation="IPASSTDDNQEITWRNFKTSNAAYSQLCEKLSLFKSVPSRLIEK
                     SAYCSIENMITNKFDVVFSFCGDDIKEFILLLPYNKSLEMY"
BASE COUNT           91 a           45 c           42 g           79 t
ORIGIN      
        1 aatccccgcc tcatcgacag atgataacca ggaaataaca tggcggaatt ttaaaactag
       61 caatgcggca tattcacaac tatgtgaaaa attatcttta tttaaaagcg tgcccagtcg
      121 gttaatagaa aaatcagctt actgctcaat tgaaaacatg atcaccaata aatttgatgt
      181 tgtcttttct ttctgcggag atgatataaa ggaatttata cttttattac cctataataa
      241 atcactggag atgtatg
//
LOCUS       DAYPHN010000473          256 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.482, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000473 DAYPHN010000000
VERSION     DAYPHN010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.482"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>256
                     /locus_tag="ACG0BW_005050"
     rRNA            <1..>256
                     /locus_tag="ACG0BW_005050"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           73 a           48 c           80 g           55 t
ORIGIN      
        1 tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg ggagccgaag
       61 ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac ccggtgatct
      121 agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg actaatgttg
      181 aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg gagatagctg
      241 gttctccccg aaagct
//
LOCUS       DAYPHN010000474          252 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.483, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000474 DAYPHN010000000
VERSION     DAYPHN010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.483"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>252
                     /locus_tag="ACG0BW_005051"
     CDS             <1..>252
                     /locus_tag="ACG0BW_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFK7261849.1"
                     /translation="GVFKECFLYRCEIKNFNIDTSKIYSVNQDPNVGKPVGAYLFMQS
                     FVYECTLLNGVCKNASIIASSLLCCKIAKLNGAEMDFIGT"
BASE COUNT           84 a           30 c           53 g           85 t
ORIGIN      
        1 cggcgtgttt aaagagtgtt ttctgtatcg atgtgaaata aaaaatttta acatagatac
       61 atcaaaaata tactctgtaa atcaagatcc caatgtgggt aagccagttg gtgcatatct
      121 gtttatgcaa tcatttgtat atgaatgtac tcttctgaat ggagtgtgta aaaatgcaag
      181 tattattgca agcagtttgt tatgttgtaa aatagcaaag cttaatggtg ctgaaatgga
      241 tttcataggg ac
//
LOCUS       DAYPHN010000475          248 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.484, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000475 DAYPHN010000000
VERSION     DAYPHN010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.484"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..>248
                     /locus_tag="ACG0BW_005052"
     rRNA            <1..>248
                     /locus_tag="ACG0BW_005052"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a           56 c           75 g           56 t
ORIGIN      
        1 cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg
       61 ctagtaatcg tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc
      121 cgtcacacca tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct
      181 taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg
      241 cggttgga
//
LOCUS       DAYPHN010000476          248 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.denovo.485, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000476 DAYPHN010000000
VERSION     DAYPHN010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.denovo.485"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..>248)
                     /locus_tag="ACG0BW_005053"
     rRNA            complement(<1..>248)
                     /locus_tag="ACG0BW_005053"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           82 c           60 g           58 t
ORIGIN      
        1 ttcctccccg ctgaaagtac tttacaaccc gaaggccttc ttcatacacg cggcatggct
       61 gcatcaggct tgcgcccatt gtgcaatatt ccccactgct gcctcccgta ggagtctgga
      121 ccgtgtctca gttccagtgt ggctggtcat cctctcagac cagctaggga tcgtcgccta
      181 ggtgagccgt taccccacct actagctaat cccatctggg cacatccgat ggcaagaggc
      241 ccgaaggt
//
LOCUS       DAYPHN010000477         3866 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.251, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000477 DAYPHN010000000
VERSION     DAYPHN010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.251"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..237
                     /locus_tag="ACG0BW_005054"
     CDS             <1..237
                     /locus_tag="ACG0BW_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFK7261850.1"
                     /translation="IGYGIAFYFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQ
                     KLDAWGFVGMGLIIAAFLLARSPSWKSLRRPTPW"
     gene            231..1070
                     /gene="sul1"
                     /locus_tag="ACG0BW_005055"
     CDS             231..1070
                     /gene="sul1"
                     /locus_tag="ACG0BW_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFK7261851.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1198..1401
                     /locus_tag="ACG0BW_005056"
     CDS             1198..1401
                     /locus_tag="ACG0BW_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFK7261852.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            1557..2762
                     /gene="chrA"
                     /locus_tag="ACG0BW_005057"
     CDS             1557..2762
                     /gene="chrA"
                     /locus_tag="ACG0BW_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFK7261853.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            2773..3078
                     /locus_tag="ACG0BW_005058"
     CDS             2773..3078
                     /locus_tag="ACG0BW_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFK7261854.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            3305..>3866
                     /locus_tag="ACG0BW_005059"
     CDS             3305..>3866
                     /locus_tag="ACG0BW_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFK7261855.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRG"
BASE COUNT          734 a         1064 c         1176 g          892 t
ORIGIN      
        1 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
       61 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
      121 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
      181 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
      241 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
      301 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
      361 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
      421 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
      481 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
      541 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
      601 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
      661 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
      721 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
      781 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
      841 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
      901 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
      961 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     1021 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     1081 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     1141 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     1201 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     1261 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     1321 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagggatc
     1381 gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc
     1441 cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat atcgaatttc
     1501 gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc gaaagtatga
     1561 cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt ctgcggcttg
     1621 gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc gaatttgtca
     1681 cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt tgtcagttct
     1741 tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg gctggataca
     1801 gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc ttgatccttt
     1861 ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg catggcttaa
     1921 aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt aacctatgca
     1981 cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt
     2041 ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg ttattgttca
     2101 agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac cgggccggcg
     2161 tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg gccgaactga
     2221 tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca ctggtgttcg
     2281 gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc ggctgggtca
     2341 acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc cctttgttca
     2401 cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg atcggcggca
     2461 ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat
     2521 tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg atcaatgccg
     2581 ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc gccatctttc
     2641 agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc tggaagctcc
     2701 cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt
     2761 gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct gcacatcctt
     2821 caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct acgccgacac
     2881 ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc
     2941 tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta tgacatcact
     3001 gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct gttcggagag
     3061 ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc ctaactggat
     3121 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
     3181 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
     3241 gtgggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     3301 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     3361 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     3421 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     3481 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     3541 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     3601 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     3661 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     3721 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     3781 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     3841 gatactgatc aagccggtgc gcggtt
//
LOCUS       DAYPHN010000478         3466 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.257, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000478 DAYPHN010000000
VERSION     DAYPHN010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.257"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..214
                     /gene="repC"
                     /locus_tag="ACG0BW_005060"
     CDS             <1..214
                     /gene="repC"
                     /locus_tag="ACG0BW_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFK7261856.1"
                     /translation="MISLSPPTICNSASIDTLCGYVWPSEASGSTMRKRRQRVREALP
                     ELVALGWTVTEFAAGKYDITRPKAAG"
     gene            522..1337
                     /gene="sul2"
                     /locus_tag="ACG0BW_005061"
     CDS             522..1337
                     /gene="sul2"
                     /locus_tag="ACG0BW_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFK7261857.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1398..2201
                     /locus_tag="ACG0BW_005062"
                     /note="aph(3'')-Ib"
     CDS             1398..2201
                     /locus_tag="ACG0BW_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFK7261858.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2201..3037
                     /locus_tag="ACG0BW_005063"
     CDS             2201..3037
                     /locus_tag="ACG0BW_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFK7261859.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3091..3327
                     /locus_tag="ACG0BW_005064"
     CDS             3091..3327
                     /locus_tag="ACG0BW_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFK7261860.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3359..>3466)
                     /locus_tag="ACG0BW_005065"
     CDS             complement(3359..>3466)
                     /locus_tag="ACG0BW_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFK7261861.1"
                     /translation="GPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT          694 a          985 c          992 g          795 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc tccatagata ccttgtgcgg
       61 ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgcc agcgggtgcg
      121 cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg cggcgggcaa
      181 gtacgacatc acccggccca aggcggcagg ctgacccccc ccactctatt gtaaacaaga
      241 catttttatc ttttatattc aatggcttat tttcctgcta attggtaata ccatgaaaaa
      301 taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag cgctgccgca
      361 cagctccata ggccgctttc ctggctttgc ttccagatgt atgctcttct gctcctgcag
      421 ctaatggatc accgcaaaca ggttactcgc ctggggattc cctttcgacc cgagcatccg
      481 tatgatactc atgctcgatt attattatta tagaagcccc catgaataaa tcgctcatca
      541 ttttcggcat cgtcaacata acctcggaca gtttctccga tggaggccgg tatctggcgc
      601 cagacgcagc cattgcgcag gcgcgtaagc tgatggccga gggggcagat gtgatcgacc
      661 tcggtccggc atccagcaat cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc
      721 gtatcgcgcc ggtgctggac gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt
      781 atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc aatgatattc
      841 gcggttttcc agacgctgcg ttctatccgc aattggcgaa atcatctgcc aaactcgtcg
      901 ttatgcattc ggtgcaagac gggcaggcag atcggcgcga ggcacccgct ggcgacatca
      961 tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt gccggtatca
     1021 aacgcaaccg ccttgtcctt gatcccggca tggggttttt tctgggggct gctcccgaaa
     1081 cctcgctctc ggtgctggcg cggttcgatg aattgcggct gcgcttcgat ttgccggtgc
     1141 ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt ccgggggatg
     1201 tcggggccgc gacactcgct gcagagcttg ccgccgccgc aggtggagct gacttcatcc
     1261 gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa
     1321 ccgcaagaat tcgttaactg cacattcggg atatttctct atattcgcgc ttcatcagaa
     1381 aactgaagga acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact
     1441 ggttgcctgt cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg
     1501 ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc
     1561 tcatttggct caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac
     1621 aggagggtgc atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg
     1681 gagcggattt gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc
     1741 tatcggttga tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg
     1801 atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc
     1861 cgcagctcga tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc
     1921 gcaccgatat ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta
     1981 aaactcttca atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg
     2041 ccgatttggc actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag
     2101 agcgcgcctt cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc
     2161 ttgccttcta tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt
     2221 tcctgctcat tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt
     2281 ttggaaagtt tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataaa
     2341 agacattgct gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc
     2401 agtccggttg ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat
     2461 gctctctcac atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact
     2521 aatggcgaag ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg
     2581 ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac
     2641 tgactacgtc cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg
     2701 tgggctacat ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt
     2761 gatagatccc gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga
     2821 tccggctgac agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc
     2881 attctctcgt gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg
     2941 gtgcctttcc gcagcttgga acgcggatgg agaagaggag caacgcgatc tagctatcgc
     3001 ggccgcgatc aagcaggtgc gacagacgtc atactagata tcaagcgact tctcctatcc
     3061 cctgggaaca catcaatctc accggagaat atcgctggcc aaagccttag cgtaggattc
     3121 cgccccttcc cgcaaacgac cccgacccgc gccacggccc acgagcggac gccgcagcag
     3181 gagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag
     3241 ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg
     3301 gatgttgtct acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc
     3361 aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc
     3421 catcgacaat caccgcgagt ccctgctcga acgctgcgtc cggacc
//
LOCUS       DAYPHN010000479         2751 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.275, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000479 DAYPHN010000000
VERSION     DAYPHN010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.275"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..255
                     /gene="frdD"
                     /locus_tag="ACG0BW_005066"
     CDS             <1..255
                     /gene="frdD"
                     /locus_tag="ACG0BW_005066"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFK7261862.1"
                     /translation="VGILLPLGLFPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCG
                     LHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            318..1451
                     /locus_tag="ACG0BW_005067"
     CDS             318..1451
                     /locus_tag="ACG0BW_005067"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFK7261863.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVCASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            1540..2073
                     /gene="blc"
                     /locus_tag="ACG0BW_005068"
     CDS             1540..2073
                     /gene="blc"
                     /locus_tag="ACG0BW_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFK7261864.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2070..2387)
                     /gene="sugE"
                     /locus_tag="ACG0BW_005069"
     CDS             complement(2070..2387)
                     /gene="sugE"
                     /locus_tag="ACG0BW_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFK7261865.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2562..2708)
                     /gene="ecnB"
                     /locus_tag="ACG0BW_005070"
     CDS             complement(2562..2708)
                     /gene="ecnB"
                     /locus_tag="ACG0BW_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFK7261866.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          679 a          712 c          689 g          671 t
ORIGIN      
        1 gtgggtattc tgctgccgct ggggctgttt ccgggtgatg cactgagcta cgagcgcgtt
       61 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
      121 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtacct
      181 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
      241 gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg
      301 tatggaaacc agaccctatg ttcaaaatga cgctctgcgc cttattaatt accgcctctt
      361 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca ataaccccgc
      421 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
      481 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
      541 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
      601 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta
      661 aacagtggaa tgggatcaca ctattacatc tcgcaaccta cactgctggc ggtctgccat
      721 tgcaggtgcc ggatgacgtt aaatcctcaa gcgacttgct gcgcttctat caaaactggc
      781 aacctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
      841 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
      901 tccagccact caaactcact catacgtgga ttaatgtgcc gtccgcagaa gaaaagaatt
      961 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gcctggggcg ttagatgctg
     1021 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     1081 aaccccttga tatcactgag aaaacgcttc aacaagggat acaactggca caatctcgct
     1141 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa
     1201 atcctgacat catcattaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa
     1261 aagcgattac gcccccaact cctgcagtat gcgcatcatg ggtacataaa acgggggcga
     1321 ccggcggatt tggtagctat gttgcgttta ttccagaaaa agagctgggt atcgtgatgc
     1381 tggctaacaa aaactatccc aatccagcga gagtcactgc cgcctggcag attctcaacg
     1441 ctctacagta aaatttcatc gggtccgaat ttttggacct tttctccgct tttccttgct
     1501 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     1561 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccccgtggc
     1621 gtgacagtag tgaataattt cgatgctaaa cgctatctcg gtacctggta tgagattgcc
     1681 cgttttgatc accgctttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgg
     1741 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagaga aatgtggcag
     1801 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     1861 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     1921 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     1981 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     2041 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     2101 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc gccgagcagc
     2161 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     2221 cctactggta acgatttcat cgcccaggca agtagcgcca tactgacaat catcgccgtc
     2281 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     2341 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     2401 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     2461 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     2521 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttactgctg cgctttcgtt
     2581 gctgcgccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     2641 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     2701 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag c
//
LOCUS       DAYPHN010000480         2650 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.278, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000480 DAYPHN010000000
VERSION     DAYPHN010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.278"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(36..785)
                     /gene="intI1"
                     /locus_tag="ACG0BW_005071"
                     /pseudo
     CDS             complement(36..785)
                     /gene="intI1"
                     /locus_tag="ACG0BW_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            943..1416
                     /gene="dfrA17"
                     /locus_tag="ACG0BW_005072"
     CDS             943..1416
                     /gene="dfrA17"
                     /locus_tag="ACG0BW_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFK7261867.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1547..2335
                     /locus_tag="ACG0BW_005073"
     CDS             1547..2335
                     /locus_tag="ACG0BW_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFK7261868.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2541..>2650
                     /locus_tag="ACG0BW_005074"
     CDS             2541..>2650
                     /locus_tag="ACG0BW_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFK7261869.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVV"
BASE COUNT          637 a          704 c          690 g          619 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gacgcacgac accgctccgt
       61 ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc acagccagga atgcccggcg
      121 cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg
      181 tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc
      241 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
      301 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
      361 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
      421 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
      481 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
      541 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
      601 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
      661 cgaacccagt ggacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc
      721 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
      781 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
      841 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      901 ggcagtcgcc ctaaaacaaa gttagccatt aagggagtta aattgaaaat atcattgatt
      961 tctgcagtgt cagaaaatgg cgtaatcggt agtggtcctg atatcccgtg gtcagtaaaa
     1021 ggtgagcaac tactctttaa agcgctcaca tataatcaat ggctccttgt cggaagaaaa
     1081 acatttgact ctatgggtgt tcttccaaat cgcaaatatg cagtagtgtc aaagaacgga
     1141 atttcaagct caaatgaaaa cgtcctagtt tttccttcaa tagaaaatgc tttgaaagag
     1201 ctatcaaaag ttacagatca tgtatatgtc tctggcgggg gtcaaatcta taatagcctt
     1261 attgaaaaag cagatataat tcatttgtct actgttcacg ttgaagtcga aggtgatatc
     1321 aaattcccta taatgcctga gaatttcaat ttggtttttg aacagttttt tatgtctaat
     1381 ataaattata cataccagat ttggaaaaaa ggctaacaat gcgttgcagc accagtcgct
     1441 tcgctccttg gacagctttt aagtcgcgtc tttgtggttt tgctgcgcaa aagtattcca
     1501 caaagccgca acttaaaagc tgccgctgaa cttaacgtta ggcatcatgg gtgaattttt
     1561 ccctgcacaa gttttcaagc agctgtccca cgctcgcgcg gtgatcgagc gccatctggc
     1621 tgcgacactg gacacaatcc acctgttcgg atctgcgatc gatggagggc tgaagccgga
     1681 cagcgacata gacttgctcg tgaccgtcag cgccgcacct aacgattcgc tccggcaggc
     1741 gctaatgctc gatttgctga aagtctcatc accgccaggc gatggcggaa catggcgacc
     1801 gctggagcta actgttgtcg ctcgaagcga agtagtgcct tggcgctatc cggcgcggcg
     1861 tgagcttcag ttcggtgagt ggctccgcca cgacatcctt tccggaacgt tcgagcctgc
     1921 cgttctggat cacgatcttg cgattttgct gaccaaggcg aggcaacaca gccttgcgct
     1981 tctaggccca tccgcagcca cgtttttcga gccggtgccg aaggagcatt tctccaaggc
     2041 gcttttcgac actattgccc agtggaatgc agagtcggat tggaagggtg acgagcggaa
     2101 cgtcgttctt gctcttgctc gcatttggta cagcgcttca actggtctca ttgctcctaa
     2161 ggacgttgct gccgcatggg tatcggagcg tttgcctgcc gagcatcggc ccctcatctg
     2221 caaggcacgc gcggcgtacc tgggtagcga ggacgacgac ctagcaatgc gcgtcgaaga
     2281 gacggccgcg ttcgttcgat atgccaaagc aacgattgag agaatcttgc gttgagcggc
     2341 atgtgcgaaa agtgcatcga cccgcgccga gggcatctga tgcctaactc ggcgttcaag
     2401 cggacgggct gcgcccgccg ctcaactatg cgttagatgc actaagcaca taattgctca
     2461 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     2521 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg
     2581 aagtaatcgc aacatccgca ttaaaatcta gcgagggctt tactaagctt gccccttccg
     2641 ccgttgtcat
//
LOCUS       DAYPHN010000481         2226 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.295, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000481 DAYPHN010000000
VERSION     DAYPHN010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.295"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..518)
                     /gene="tetR"
                     /locus_tag="ACG0BW_005075"
     CDS             complement(<1..518)
                     /gene="tetR"
                     /locus_tag="ACG0BW_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR"
                     /protein_id="HFK7261870.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEA"
     gene            624..1823
                     /gene="tet(A)"
                     /locus_tag="ACG0BW_005076"
     CDS             624..1823
                     /gene="tet(A)"
                     /locus_tag="ACG0BW_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFK7261871.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1855..2187)
                     /locus_tag="ACG0BW_005077"
                     /pseudo
     CDS             complement(1855..2187)
                     /locus_tag="ACG0BW_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
BASE COUNT          393 a          688 c          689 g          456 t
ORIGIN      
        1 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
       61 gtgccgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
      121 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
      181 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
      241 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
      301 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
      361 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
      421 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
      481 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
      541 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
      601 ggaggctggt ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca
      661 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
      721 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
      781 cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
      841 ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg
      901 cgcctttcct ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg
      961 gggcggtagc cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact
     1021 tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc
     1081 tgatgggcgg tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca
     1141 atttcctgac gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac
     1201 gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg
     1261 ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt
     1321 gggtcatttt cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg
     1381 ccgcatttgg cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc
     1441 ggctcggcga aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc
     1501 tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg
     1561 gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc
     1621 aggggcagct gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc
     1681 tcctcttcac ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg
     1741 caggcgctgc cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg
     1801 cagggcaacg agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg
     1861 cgacttccgg caagctatac gcgccctagg agtgcggttg gaacgttggc ccagccagat
     1921 actcccgatc acgagcagga cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa
     1981 caaccatcct agcaacacgg cggtccccgg gctgagaaag cccagtaagg aaacaactgt
     2041 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
     2101 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa
     2161 cactaaagct actggaacga gcagaagggc actgttgcaa agttagcgat gaggcagcct
     2221 tttgtc
//
LOCUS       DAYPHN010000482         2055 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.306, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000482 DAYPHN010000000
VERSION     DAYPHN010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.306"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            109..1014
                     /locus_tag="ACG0BW_005078"
     CDS             109..1014
                     /locus_tag="ACG0BW_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFK7261872.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..>2055
                     /gene="mrx(A)"
                     /locus_tag="ACG0BW_005079"
                     /gene_synonym="mrx"
     CDS             1011..>2055
                     /gene="mrx(A)"
                     /locus_tag="ACG0BW_005079"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFK7261873.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSA"
BASE COUNT          306 a          659 c          673 g          417 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcgg
//
LOCUS       DAYPHN010000483         2027 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.309, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000483 DAYPHN010000000
VERSION     DAYPHN010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.309"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            <1..727
                     /locus_tag="ACG0BW_005080"
     CDS             <1..727
                     /locus_tag="ACG0BW_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFK7261874.1"
                     /translation="RHLAATLDTIHLFGSAIDGGLKPDSDIDLLVTVSAAPNDSLRQA
                     LMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYPARRELQFGEWLRHDILSGTFE
                     PAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKEHFSKALFDTIAQWNAESDWKG
                     DERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPAEHRPLICKARAAYLGSEDDDL
                     AMRVEETAAFVRYAKATIERILR"
     gene            933..1280
                     /locus_tag="ACG0BW_005081"
     CDS             933..1280
                     /locus_tag="ACG0BW_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFK7261875.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1274..>2027
                     /gene="folP"
                     /locus_tag="ACG0BW_005082"
     CDS             1274..>2027
                     /gene="folP"
                     /locus_tag="ACG0BW_005082"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFK7261876.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPG"
BASE COUNT          387 a          583 c          588 g          469 t
ORIGIN      
        1 gcgccatctg gctgcgacac tggacacaat ccacctgttc ggatctgcga tcgatggagg
       61 gctgaagccg gacagcgaca tagacttgct cgtgaccgtc agcgccgcac ctaacgattc
      121 gctccggcag gcgctaatgc tcgatttgct gaaagtctca tcaccgccag gcgatggcgg
      181 aacatggcga ccgctggagc taactgttgt cgctcgaagc gaagtagtgc cttggcgcta
      241 tccggcgcgg cgtgagcttc agttcggtga gtggctccgc cacgacatcc tttccggaac
      301 gttcgagcct gccgttctgg atcacgatct tgcgattttg ctgaccaagg cgaggcaaca
      361 cagccttgcg cttctaggcc catccgcagc cacgtttttc gagccggtgc cgaaggagca
      421 tttctccaag gcgcttttcg acactattgc ccagtggaat gcagagtcgg attggaaggg
      481 tgacgagcgg aacgtcgttc ttgctcttgc tcgcatttgg tacagcgctt caactggtct
      541 cattgctcct aaggacgttg ctgccgcatg ggtatcggag cgtttgcctg ccgagcatcg
      601 gcccctcatc tgcaaggcac gcgcggcgta cctgggtagc gaggacgacg acctagcaat
      661 gcgcgtcgaa gagacggccg cgttcgttcg atatgccaaa gcaacgattg agagaatctt
      721 gcgttgagcg gcatgtgcga aaagtgcatc gacccgcgcc gagggcatct gatgcctaac
      781 tcggcgttca agcggacggg ctgcgcccgc cgctcaacta tgcgttagat gcactaagca
      841 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
      901 taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg
      961 caatagttgg cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc
     1021 ttgccccttc cgccgttgtc ataatcggtt atggcatcgc attttatttt ctttctctgg
     1081 ttctgaaatc catccctgtc ggtgttgctt atgcagtctg gtcgggactc ggcgtcgtca
     1141 taattacagc cattgcctgg ttgcttcatg ggcaaaagct tgatgcgtgg ggctttgtag
     1201 gtatggggct cataattgct gcctttttgc tcgcccgatc cccatcgtgg aagtcgctgc
     1261 ggaggccgac gccatggtga cggtgttcgg cattctgaat ctcaccgagg actccttctt
     1321 cgatgagagc cggcggctag accccgccgg cgctgtcacc gcggcgatcg aaatgctgcg
     1381 agtcggatca gacgtcgtgg atgtcggacc ggccgccagc catccggacg cgaggcctgt
     1441 atcgccggcc gatgagatca gacgtattgc gccgctctta gacgccctgt ccgatcagat
     1501 gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag cgctatgcgc tcaagcgcgg
     1561 cgtgggctac ctgaacgata tccaaggatt tcctgaccct gcgctctatc ccgatattgc
     1621 tgaggcggac tgcaggctgg tggttatgca ctcagcgcag cgggatggca tcgccacccg
     1681 caccggtcac cttcgacccg aagacgcgct cgacgagatt gtgcggttct tcgaggcgcg
     1741 ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg ctcatcctcg atccggggat
     1801 gggatttttc ttgagccccg caccggaaac atcgctgcac gtgctgtcga accttcaaaa
     1861 gctgaagtcg gcgttggggc ttccgctatt ggtctcggtg tcgcggaaat ccttcttggg
     1921 cgccaccgtt ggccttcctg taaaggatct gggtccagcg agccttgcgg cggaacttca
     1981 cgcgatcggc aatggcgctg actacgtccg cacccacgcg cctggag
//
LOCUS       DAYPHN010000484         1967 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.311, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000484 DAYPHN010000000
VERSION     DAYPHN010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.311"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            complement(<1..879)
                     /gene="pepD"
                     /locus_tag="ACG0BW_005083"
     CDS             complement(<1..879)
                     /gene="pepD"
                     /locus_tag="ACG0BW_005083"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HFK7261877.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAE"
     gene            1140..1598
                     /gene="gpt"
                     /locus_tag="ACG0BW_005084"
     CDS             1140..1598
                     /gene="gpt"
                     /locus_tag="ACG0BW_005084"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFK7261878.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1690..>1967
                     /locus_tag="ACG0BW_005085"
     CDS             1690..>1967
                     /locus_tag="ACG0BW_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFK7261879.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYR"
BASE COUNT          480 a          510 c          486 g          491 t
ORIGIN      
        1 ctctgccagc tcgtttttca ggatttcctg ataggtattc accagagatt tcagggcgtc
       61 gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag
      121 tgtgccaccg ttgaaatcga taaggcgcag gtccagctct tctgcatgac ccgccaggaa
      181 gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc
      241 gccttttaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ctgcttcacg
      301 atctaaatgc aggttggagg tgaagtcgat accccccgcg caacccatgt agatttcacc
      361 ttcctcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc
      421 aaaagcacca tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg
      481 aaccacgttt tcgtcagcca gaaccgccag cgcagacgcc ataccaatgc cgttatccgc
      541 acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc
      601 tttagtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg
      661 caagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt
      721 acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc
      781 agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc
      841 ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca
      901 cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg
      961 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     1021 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     1081 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     1141 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     1201 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg
     1261 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     1321 ccagctatga tcacgacaac cagcgcgagc ttaaagtgct aaaacgcgca gaaggcgatg
     1381 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggcactgcg gttgcgattc
     1441 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     1501 tggttgatga ttatgttgtt gatatcccgc aagatacctg gatcgaacaa ccgtgggata
     1561 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     1621 cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag tattctggag
     1681 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     1741 cctgaaacct cgacgctagt tcgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     1801 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     1861 tggcgcggca ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     1921 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccg
//
LOCUS       DAYPHN010000485         1483 bp    DNA     linear   BCT 17-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201516
            SAMEA115553451-rid22447913.guided.335, whole genome shotgun
            sequence.
ACCESSION   DAYPHN010000485 DAYPHN010000000
VERSION     DAYPHN010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115553451
            Sequence Read Archive: ERR13185714
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JUL-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002269490.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002269490.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/16/2024 19:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 94 of 141
            Pseudo Genes (internal stop)      :: 31 of 141
            Pseudo Genes (multiple problems)  :: 33 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115553451-rid22447913.guided.335"
                     /isolate="2201516"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Netherlands"
                     /collection_date="2018-01-01"
     gene            175..1050
                     /locus_tag="ACG0BW_005086"
     CDS             175..1050
                     /locus_tag="ACG0BW_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-27"
                     /protein_id="HFK7261880.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1085..>1483
                     /locus_tag="ACG0BW_005087"
     CDS             1085..>1483
                     /locus_tag="ACG0BW_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFK7261881.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNMALLQIVGGDKLII"
BASE COUNT          361 a          372 c          427 g          323 t
ORIGIN      
        1 gatgaaatca atgatttatc aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt
       61 gtgagaagca gtctaaattc ttcgtgaaat agtgattttt gaagctaata aaaaacacac
      121 gtggaattta gggaatactg atgtaacacg gattgaccgt attgggagtt tgagatggtg
      181 acaaagagag tgcaacggat gatgttcgcg gcggcggcgt gcattccgct gctgctgggc
      241 agcgcgccgc tttatgcgca gacgagtgcg gtgcagcaaa agctggcggc gctggagaaa
      301 agcagcggag ggcggctggg cgtcgcgctc atcgataccg cagataatac gcaggtgctt
      361 tatcgcggtg atgaacgctt tccaatgtgc agtaccagta aagttatggc ggccgcggcg
      421 gtgcttaagc agagtgaaac gcaaaagcag ctgcttaatc agcctgtcga gatcaagcct
      481 gccgatctgg ttaactacaa tccgattgcc gaaaaacacg tcaacggcac aatgacgctg
      541 gcagaactga gcgcggccgc gttgcagtac agcgacaata ccgccatgaa caaattgatt
      601 gcccagctcg gtggcccggg aggcgtgacg gcttttgccc gcgcgatcgg cgatgagacg
      661 tttcgtctgg atcgcactga acctacgctg aataccgcca ttcccggcga cccgagagac
      721 accaccacgc cgcgggcgat ggcgcagacg ttgcgtcagc ttacgctggg tcatgcgctg
      781 ggcgaaaccc agcgggcgca gttggtgacg tggctcaaag gcaatacgac cggcgcagcc
      841 agcattcggg ccggcttacc gacgtcgtgg actgtgggtg ataagaccgg cagcggcggc
      901 tacggcacca ccaatgatat tgcggtgatc tggccgcagg gtcgtgcgcc gctggttctg
      961 gtgacctatt ttacccagcc gcaacagaac gcagagagcc gccgcgatgt gctggcttca
     1021 gcggcgagaa tcatcgccga agggctgtaa ctggttttgt tgaataaatc gaacttttgc
     1081 tgagttgaag gatcagatca cgtatcttcc cgacaacgca gaccgttccg tggcaaagca
     1141 aaagttcaaa atcaccaact ggcccaccta caataaagcc ctcatcaacc gtggctccat
     1201 aactttctgg ctggatgatg aagctattca ggcctggtat gagtcagcaa caccttcttc
     1261 acgaggcaga cctcagcgct attctgacct tgccatcacg actgtgctgg tcattaaacg
     1321 cgtattcagg ctgaccctgc gcgctgcgca gggctttatt gattccattt tttctctgat
     1381 gaacgttccg ctacgctgcc cggattacag ctgtgtcagc aggcgggcaa agtcggttaa
     1441 tatggcactg ttgcaaatag tcggtggtga taaacttatc atc
//
